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Sample records for cdc americano sistema

  1. ¿Es aplicable el sistema de vigilancia epidemiológica de las infecciones intrahospitalarias que recomienda el CDC- americano (sistema NNIS) en un hospital chileno?

    OpenAIRE

    Naldy Febré V; Eduardo S de Medeiros; Sergio B Wey; Maruza Larrondo P; Víctor Silva V

    2001-01-01

    Background: The National Nosocomial Infections Surveillance System (NNIS system) is the method for surveying nosocomial infections used by the Centers for Disease Control (CDC). This strategy allows the comparison of different hospitals, using rate adjustments. In Chile, this system is not used. Aim: To report the application of this system in a tertiary reference hospital in Chile. Materials and methods: We performed a six months prospective cohort study. The active surveillance was carried ...

  2. ¿Es aplicable el sistema de vigilancia epidemiológica de las infecciones intrahospitalarias que recomienda el CDC- americano (sistema NNIS) en un hospital chileno? Application of the National Nosocomial Infections Surveillance System recommended by the CDC, in a Chilean hospital

    OpenAIRE

    Naldy Febré V; Eduardo S de Medeiros; Sergio B Wey; Maruza Larrondo P; Víctor Silva V

    2001-01-01

    Background: The National Nosocomial Infections Surveillance System (NNIS system) is the method for surveying nosocomial infections used by the Centers for Disease Control (CDC). This strategy allows the comparison of different hospitals, using rate adjustments. In Chile, this system is not used. Aim: To report the application of this system in a tertiary reference hospital in Chile. Materials and methods: We performed a six months prospective cohort study. The active surveillance was carried ...

  3. Sistema privado de seguro-saúde: lições do caso americano

    Directory of Open Access Journals (Sweden)

    Mônica Viegas Andrade

    2000-01-01

    Full Text Available O mercado de serviços de atenção à saúde apresenta particularidades não observadas em outros mercados de bens, traduzidas nas relações contratuais estabelecidas entre os agentes deste mercado: relação paciente-provedor e relação seguradora-provedor. Na relação seguradora-provedor existe um problema de risco moral que cria incentivos à sobreutilização de serviços médicos. O objetivo deste trabalho é sistematizar as principais características institucionais do sistema de saúde americano, enfatizando as reformas ocorridas na década de 1970 e os problemas identificados em regulamentar o setor privado de serviços médicos e hospitalares. Estes problemas estão relacionados, em sua grande maioria, à regulamentação dos contratos permitidos entre as seguradoras, os provedores de serviços médicos e hospitalares e os segurados. A principal conclusão deste trabalho é mostrar que a flexibilização dos contratos entre provedores e seguradora tem efeito significativo na redução dos custos do seguro-saúde, resultando sobretudo em maior eficiência dos serviços ofertados. Os novos desenhos de contratos estabelecidos permitem a divisão do risco entre os provedores e seguradora, além de centralizar a decisão de encaminhamentos médicos a serem realizados configurando utilização mais racional destes serviços. A flexibilização dos contratos não significou, no entanto, eliminação da regulação por parte do Estado do setor de seguro-saúde privado, sendo fundamental para garantir a sustentabilidade financeira dos seguros.The aim of this paper is to organize the main institutional characteristics of the American Health System, emphasizing the reforms that occurred during the age of seventy and the difficulties to regulate the private sector of medical and hospital services. The most part of these problems are related to the contract regulation between the providers of medical and hospital service and the patients. The

  4. La justicia constitucional ante el siglo XXI: la progresiva convergencia de los sistemas americano y europeo-kelseniano (extracto del libro: La Constitución Española en el contexto constitucional europeo. Editor: Francisco Fernández Segado

    Directory of Open Access Journals (Sweden)

    Francisco Fernández Segado

    2014-01-01

    Full Text Available 1. Reflexiones preliminares 2. La última «ratio» de la bipolaridad: la concepción del «legislador negativo» y la reafirmación kelseniana del principio de sujeción de los jueces a la ley 3. La obsolescencia de la clásica polaridad «sistema americano» versus «sistema europeo-kelseniano» 4. La centralidad del modelo americano en el proceso de convergencia de los dos tradicionales sistemas de control de constitucionalidad 5. La quiebra frontal de la concepción kelseniana del «legislador negativo» 6. La mixtura e hibridación de los actuales sistemas jurisdiccionales de control de constitucionalidad 7. A la búsqueda de una nueva tipología explicativa de las variables conformadoras del control de la constitucionalidad

  5. Nota técnica sobre el sistema nacional de innovación de Costa Rica: Una contribución al diálogo de políticas públicas entre el Gobierno de La República de Costa Rica y el Banco Inter-Americano de Desarrollo

    OpenAIRE

    Gustavo Crespi; Rodrigo Fuentes; Roberto Alvarez; Jeffrey Orozco

    2010-01-01

    La siguiente nota técnica presenta una revisión del sistema de innovación de Costa Rica con especial énfasis en sus principales fortalezas y debilidades. El objetivo de la misma es contribuir al diálogo de políticas entre el Gobierno de Costa Rica y el Banco Inter- Americano de Desarrollo (BID) de cara al próximo período presidencial. El marco conceptual adoptado para la presente revisión esencialmente parte del concepto de innovación como la introducción de productos, procesos, servicios o m...

  6. El escritor americano

    Directory of Open Access Journals (Sweden)

    Cristina Iglesia

    2006-04-01

    Full Text Available Facundo se escribe para los no saben leer América. Este es uno de los puntos de partida de Sarmiento : hay una mala lectura europea de América, sobre todo de la Argentina, del Río de la Plata, de esta parte de América que es, en realidad, la única que a Sarmiento le interesa. “Sus más hábiles políticos no han alcanzado a comprender nada de lo que sus ojos han visto al echar una mirada precipitada sobre el poder americano que desafiaba a la gran nación” escribe en Viajes.Y Europa no sabe leer ...

  7. Sistema

    OpenAIRE

    Martín Serrano, Manuel

    1982-01-01

    Los términos "Modelo" y "Sistema" son dos temas escritos por Manuel Martín Serrano para la docencia universitaria. De cada uno de ellos, se ha depositado en E-Prints la versión más difundida. Proporcionan conceptos y categorías que hacen posible la aplicación de las metodologías sistémicas en la investigación de las ciencias sociales. El capítulo "Sistema" incluye los siguientes epígrafes: I. El estudio de la organización y el funcionamiento de los sistemas: 1. Concepto de sistema. 2. Análisi...

  8. sistemas

    Directory of Open Access Journals (Sweden)

    Diego Alejandro Muñoz Gaviria

    2006-01-01

    Full Text Available El texto presentado a continuación, plantea en términos generales las principales posturas conceptuales del sociólogo alemán Niklas Luhmann sobre las ideas de Max Weber y su teoría de la acción social, y de Talcott Parsons y sus planteamientos en torno a la teoría de los sistemas sociales. Al final del escrito se hace especial énfasis en el rol protagónico que para Luhmann tienen las ciencias sociales y humanas en la autorreferencia del sistema social.

  9. Pela democracia dos sistemas de avaliação da produção acadêmica: convergências de estudiosos latino-americanos e europeus

    Directory of Open Access Journals (Sweden)

    Aimée Vega Montiel

    2013-01-01

    Full Text Available El presente texto es la transcripción de una conferencia pronunciada en 2011, en el encuentro anual de la Europeean Comunication Research and Education Association (ECREA, que plantea la discusión sobre los efectos de la supremacía de los investigadores del norte sobre los investigadores del sur en el contexto de la evaluación de la calidad de la producción académica. Aunque se reconozcan las especificidades locales y regionales, así como la necesidad de adopción de diferentes metodologías e instrumentos de investigación para el estudio de problemas distintos, en contextos sociales distintos, la producción académica de los estudiosos latinoamericanos sigue siendo sometida a patrones definidos en otras latitudes del mundo. Se argumenta que la supremacía científica de los investigadores del norte se debe, fundamentalmente, a la supremacía del inglés en el sistema académico internacional, que limita las posibilidades de diseminación del conocimiento en otras lenguas.

  10. La Escuela de Estudios Hispano-Americanos

    OpenAIRE

    Bernabéu Albert, Salvador

    2010-01-01

    La Escuela de Estudios Hispano-Americanos es una institución cultural, perteneciente al Consejo Superior de Investigaciones Científicas, dedicada al estudio del Americanismo desde 1942 a la actualidad. Está encuadrada en el sector de Humanidades, una de las ocho áreas científico-técnicas en que se organiza el CSIC, el mayor organismo público de investigación en España. Paralelamente a los profundos cambios que están ocurriendo en la Universidad española con el Plan de ...

  11. CDC Disease Detective Camp

    Centers for Disease Control (CDC) Podcasts

    2010-08-02

    The CDC Disease Detective Camp gives rising high school juniors and seniors exposure to key aspects of the CDC, including basic epidemiology, infectious and chronic disease tracking, public health law, and outbreak investigations. The camp also helps students explore careers in public health.  Created: 8/2/2010 by Centers for Disease Control and Prevention (CDC).   Date Released: 8/2/2010.

  12. CDC Vital Signs: Binge Drinking

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  13. CDC Vital Signs: Child Injury

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  14. CDC Child Growth Charts

    Data.gov (United States)

    U.S. Department of Health & Human Services — CDC child growth charts consist of a series of percentile curves that illustrate the distribution of selected body measurements in U.S. children. Pediatric growth...

  15. CDC WONDER: Births

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Births (Natality) online databases in CDC WONDER report birth rates, fertility rates and counts of live births occurring within the United States to U.S....

  16. Entrecruzamientos hispano-americanos en la Universidad Central

    OpenAIRE

    López-Ocón, Leoncio

    2013-01-01

    Desde los planteamientos metodológicos de l'histoire croisée se analizan las interacciones entre universitarios españoles e hispano-americanos que se produjeron en un singular "punto de intersección" de la Universidad Central de la Segunda República española, como fue su Facultad de Derecho. Se presta una particular atención al viaje del mexicano Daniel Cosio Villegas al Madrid de 1933, y a las relaciones de Silvio Zavala con Rafael Altamira en el marco de un seminario americanista. Y se sost...

  17. Recensão de "Cadernos de estudos latino-americanos nº4"

    OpenAIRE

    Cardoso, João Casqueira

    2010-01-01

    Recensão - TOSCANO, Ana María da Costa, org. - Cadernos de estudos latino-americanos : publicação do CELA, Centro de Estudos Latino-Americanos. Porto: Edições Universidade Fernando Pessoa, 2008. p. 134. ISSN 1646-5164

  18. Announcement: CDC's 70th Anniversary.

    Science.gov (United States)

    2016-01-01

    July 1, 2016, marks the 70th anniversary of the establishment of CDC. Since the agency's launch as the Communicable Disease Center on July 1, 1946, CDC's primary mission has been improving public health in the United States and around the world through prevention and preparedness. In the seven decades since CDC's founding, the agency has grown in size and mission and is recognized as the nation's premiere health promotion, disease prevention, and emergency preparedness agency, and a global leader in public health. CDC's mission has progressed beyond communicable disease control and now encompasses noninfectious diseases, injury prevention, and environmental and occupational health. PMID:27362420

  19. CDC Lab Values

    Centers for Disease Control (CDC) Podcasts

    2015-02-02

    More than fifteen hundred scientists fill the lab benches at CDC, logging more than four million hours each year. CDC’s laboratories play a critical role in the agency’s ability to find, stop, and prevent disease outbreaks. This podcast provides a brief overview of what goes on inside CDC’s labs, and why this work makes a difference in American’s health.  Created: 2/2/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 2/2/2015.

  20. Tragédia Grega e Western Americano

    OpenAIRE

    Simôes Rodrigues, Nuno

    2015-01-01

    A relação que se pode estabelecer entre a cultura grega e o western americano não é uma novidade hermenêutica. Na verdade, já vários autores (e.g. P. L. Cano) chamaram a atenção para a proximidade da estrutura de filmes como High Noon, de Fred Zinnemann (1952), por exemplo, e a tragédia ática, ou The Searchers, de John Ford (1956), e a epopeia homérica. Em alguns casos, ainda que por entreposto meio (teatro de E. O’Neill), verifica-se mesmo uma reutilização do próprio texto trágico e sua adap...

  1. CDC Vital Signs: Prescription Painkiller Overdoses

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  2. CDC Vital Signs: Alcohol and Pregnancy

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  3. CDC Vital Signs: Asthma in the US

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  4. CDC Vital Signs: Making Health Care Safer

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  5. CDC Vital Signs: Preventing Norovirus Outbreaks

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  6. Encontrando os feminismos latino-americanos e caribenhos

    Directory of Open Access Journals (Sweden)

    Alvarez Sonia E

    2003-01-01

    Full Text Available Este artigo examina os Encontros Latino-Americanos e do Caribe como espaços críticos transnacionais onde se re-imagina a política dos feminismos na região. Enfocando o Oitavo desses Encontros, realizado em Juan Dolio, República Dominicana, em 1999, analisamos os principais debates politicos e filosóficos que surgiram durante 20 anos de Encontros: (1 mudanças nas concepções de 'autonomia' do movimento e na relação dos feminismos com o movimento de mulheres mais amplo e com outros atores na sociedade civil e política, o Estado e instituições internacionais; (2 controvérsias geradas pelas recorrentes crises de 'inclusão' e de 'expansão' do movimento; e (3 debates centrados nas diferenças, desigualdades e desequilíbrios de poder entre mulheres em geral e entre as feministas em particular.

  7. CDC PRAMStat Data for 2002

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  8. CDC PRAMStat Data for 2010

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  9. CDC PRAMStat Data for 2011

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  10. CDC PRAMStat Data for 2000

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  11. CDC PRAMStat Data for 2001

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  12. CDC PRAMStat Data for 2006

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  13. CDC PRAMStat Data for 2003

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  14. CDC PRAMStat Data for 2004

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  15. CDC PRAMStat Data for 2007

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  16. CDC PRAMStat Data for 2008

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  17. CDC PRAMStat Data for 2009

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  18. CDC PRAMStat Data for 2005

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  19. CDC WONDER: Mortality - Infant Deaths

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Infant Deaths (from Linked Birth / Infant Death Records) online databases on CDC WONDER provide counts and rates for deaths of children under 1 year...

  20. A dinâmica do novo regime monetário-financeiro norte-americano: uma hipótese de interpretação

    Directory of Open Access Journals (Sweden)

    Marcos Antonio Macedo Cintra

    2000-08-01

    Full Text Available No artigo é apresentada uma hipótese de interpretação sobre a dinâmica atual do sistema monetário e financeiro norte-americano, suas relações com as empresas, com o Federal Reserve, com o Tesouro e com o resto do mundo. Procurou-se mostrar a emergência de um modo de funcionamento específico do capitalismo predominantemente financeiro e rentista, em que prevalecem os mercados de capitais sofisticados e abrangentes, destinados a uma avaliação diária da massa de ativos (cambial, acionário, de títulos e imobiliário. Ademais, essa forma de gestão da riqueza se impôs ao resto do mundo como paradigma: as finanças passaram a operar num espaço mundial, hierarquizado a partir do sistema financeiro norte-americano e viabilizado pela política monetária do Estado hegemônico, imitada pelos demais países industrializados e em desenvolvimento.The article is an interpretation of the current dynamics of the North American monetary system, and its relations with enterprises, the Federal Reserve system, the Treasury and the rest of the world. The author looked into the emergence of a specific mode of operation of a predominantly financial, unearned income-based type of capitalism, in which sophisticated, encompassing capital markets, forced to perform a daily evaluation of assets (foreign exchange, shares, securities, and property prevail. Moreover, this way of managing resources has become a paradigm for the rest of the world: financial operations started to occur in a global space, stemming hierarchically from the North American financial system, and being made possible by the monetary policies of the hegemonic nation, and imitated by developed and developing countries.

  1. CDC Kerala - The Untold Story.

    Science.gov (United States)

    Nair, M K C; Leela, Leena Mundapalliyil; George, Babu; Bhaskaran, Deepa; Pillai, Asokan Nataraja; Sarasamma, Harikumaran Nair Gopinathan Nair

    2016-05-01

    This article is our life time experience in conceptualizing and systematically developing Child Development Centre (CDC) Kerala in the last 25 years, from a research project to a national training centre in child and adolescent development and premarital counseling. CDC Kerala's major contribution was in creating a 'conceptual framework' of a valid link between childhood disability, low birth weight, adolescent girls' nutrition and fetal onset adult lifestyle diseases. It all started with a randomized controlled trial (RCT) proving beyond doubt that early stimulation is effective in improving the neurodevelopmental status of high risk babies at one and two years and the same cohort was followed-up in detail at 5, 13, 16, 19 and 24 completed years. The process of establishing CDC Kerala is being presented under (i) clinical child development, (ii) adolescent care counseling, (iii) young adults and premarital counseling and (iv) institution building. PMID:26988580

  2. FUTEBOL AMERICANO NO PAÍS DO FUTEBOL: O CASO DO CUIABÁ ARSENAL

    OpenAIRE

    Francisco Xavier Freire Rodrigues; Neuza Cristina Gomes Costa; Lenara da Costa Pedroso; Joycy Ambrósio da Silva

    2015-01-01

    O estudo aborda o processo de esportivização do futebol americano em Mato Grosso a partir da trajetória do Cuiabá Arsenal. Trata-se de uma pesquisa qualitativa, exploratória, com delineamento de estudo de caso. Dentre os resultados, verifica-se que a esportivização do Futebol Americano em Cuiabá deu-se por fases: amadorismo, transição, profissional. A equipe inspirou a criação de outras agremiações. Mesmo em época preparativa para Copa do Mundo, com intensa publicidade pelo governo do estado ...

  3. Stalking SARS: CDC at Work

    Centers for Disease Control (CDC) Podcasts

    2014-05-22

    In this podcast for kids, the Kidtastics talk about the SARS outbreak and how CDC worked to solve the mystery.  Created: 5/22/2014 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 5/22/2014.

  4. CDC Vital Signs: Legionnaires' Disease

    Science.gov (United States)

    ... Overview CDC investigated the first outbreak of Legionnaires’ disease, a serious lung infection (pneumonia), in 1976. An increasing number ... they develop symptoms of pneumonia. Test for Legionnaires’ disease in people with serious pneumonia, especially those requiring intensive care or who ...

  5. The polo-like kinase Cdc5 interacts with FEAR network components and Cdc14

    OpenAIRE

    Rahal, Rami; Amon, Angelika

    2008-01-01

    Exit from mitosis in Saccharomyces cerevisiae is triggered by activation of the phosphatase Cdc14. Throughout interphase and early mitosis, Cdc14 is sequestered in the nucleolus by its inhibitor Cfi1/Net1. In anaphase, the Cdc Fourteen Early Anaphase Release (FEAR) network and the Mitotic Exit Network (MEN) coordinately trigger the release of Cdc14 from the nucleolus. Here we show that the FEAR network component Cdc5 physically associates with two other members of the pathway, the Separase Es...

  6. y jurisprudenciales en el ámbito americano

    Directory of Open Access Journals (Sweden)

    Aníbal Quiroga León

    2005-01-01

    Full Text Available El artículo analiza la protección de los derechos fundamentales en el marco de las relaciones entre derecho internacional y derecho interno, teniendo como punto central el sistema interamericano y sus órganos de aplicación, La Comisión y la Corte Interamericana de Derechos Humanos, considerando aspectos procedimentales y la adopción de medidas cautelares por esta última. Finalmente, se analiza la superioridad del derecho internacional de los derechos humanos sobre el derecho interno, teniendo como fundamento los casos “Barrios Altos” (Chumbipoma Aguirre y otros, cuya sentencia es del 14 de marzo del 2001 y el caso“La última tentación de Cristo” (Olmedo Bustos y otros cuya sentencia es del 5 de febrero del 2001.

  7. Functional characterization and cellular dynamics of the CDC-42 - RAC - CDC-24 module in Neurospora crassa.

    Directory of Open Access Journals (Sweden)

    Cynthia L Araujo-Palomares

    Full Text Available Rho-type GTPases are key regulators that control eukaryotic cell polarity, but their role in fungal morphogenesis is only beginning to emerge. In this study, we investigate the role of the CDC-42 - RAC - CDC-24 module in Neurospora crassa. rac and cdc-42 deletion mutants are viable, but generate highly compact colonies with severe morphological defects. Double mutants carrying conditional and loss of function alleles of rac and cdc-42 are lethal, indicating that both GTPases share at least one common essential function. The defects of the GTPase mutants are phenocopied by deletion and conditional alleles of the guanine exchange factor (GEF cdc-24, and in vitro GDP-GTP exchange assays identify CDC-24 as specific GEF for both CDC-42 and RAC. In vivo confocal microscopy shows that this module is organized as membrane-associated cap that covers the hyphal apex. However, the specific localization patterns of the three proteins are distinct, indicating different functions of RAC and CDC-42 within the hyphal tip. CDC-42 localized as confined apical membrane-associated crescent, while RAC labeled a membrane-associated ring excluding the region labeled by CDC42. The GEF CDC-24 occupied a strategic position, localizing as broad apical membrane-associated crescent and in the apical cytosol excluding the Spitzenkörper. RAC and CDC-42 also display distinct localization patterns during branch initiation and germ tube formation, with CDC-42 accumulating at the plasma membrane before RAC. Together with the distinct cellular defects of rac and cdc-42 mutants, these localizations suggest that CDC-42 is more important for polarity establishment, while the primary function of RAC may be maintaining polarity. In summary, this study identifies CDC-24 as essential regulator for RAC and CDC-42 that have common and distinct functions during polarity establishment and maintenance of cell polarity in N. crassa.

  8. FUTEBOL AMERICANO NO PAÍS DO FUTEBOL: O CASO DO CUIABÁ ARSENAL

    Directory of Open Access Journals (Sweden)

    Francisco Xavier Freire Rodrigues

    2015-01-01

    Full Text Available O estudo aborda o processo de esportivização do futebol americano em Mato Grosso a partir da trajetória do Cuiabá Arsenal. Trata-se de uma pesquisa qualitativa, exploratória, com delineamento de estudo de caso. Dentre os resultados, verifica-se que a esportivização do Futebol Americano em Cuiabá deu-se por fases: amadorismo, transição, profissional. A equipe inspirou a criação de outras agremiações. Mesmo em época preparativa para Copa do Mundo, com intensa publicidade pelo governo do estado para divulgar o futebol “brasileiro”, o futebol “americano” é assunto da agenda esportiva midiática e cotidiana. Fato que demonstra como é possível ser esportivo.

  9. Orquestrando vozes de gênero de estudantes estrangeiros/as latino-americanos/as

    Directory of Open Access Journals (Sweden)

    AlencarRodrigues, Roberta de

    2010-01-01

    Full Text Available Esta pesquisa discute efeitos de sentidos produzidos pelos/as estudantes estrangeiros/as latino-americanos/as acerca das concepções de gênero no país de origem e no Brasil. Fundamenta-se nos estudos de gênero e no conceito de aculturação proposto pela Psicologia Intercultural para abordar as mudanças de valores de gênero resultantes do contato com uma nova cultura. Aponta para as questões da linguagem e do discurso, ancorados na análise de discurso (AD, desenvolvida por Michel Pêcheux, utilizando sequências discursivas produzidas por estudantes estrangeiros/as latino-americanos/as. As conclusões indicam que o tema da sexualidade marca o discurso dos/as participantes da pesquisa no que concerne às relações de gênero no Brasil.

  10. PROTAGONISMO DOS ÍNDIOS NORTE-AMERICANOS NOS DESENHOS ANIMADOS DE WALTER LANTZ

    Directory of Open Access Journals (Sweden)

    RAFAEL DUARTE OLIVEIRA VENANCIO

    2011-06-01

    Full Text Available Condemned to a dubious media representation in early cinema through thenovels of the late nineteenth century, the North American Indians never had a high profile infilms of this period. However, an animator, Walter Lantz, has a high degree of characterizationof Native Americans in his work. The aim of this paper is to study the role of Native Americansin these cartoons and analyze the social implications of these choices, using the concepts ofapparatus and formula. Furthermore, the analysis will include a portrayal of the Americanmedia scene before and after Lantz, seeking disruptions and legacies.Condenados a uma duvidosa representação midiática no início do cinema graçasaos romances do final do século XIX, os índios norte-americanos nunca tiveram um grandedestaque nos filmes dessa época. No entanto, um realizador de desenhos animados, WalterLantz, possui um alto grau de caracterização de nativos norte-americanos em seus trabalhos. Oobjetivo do presente artigo é estudar o protagonismo dos índios norte-americanos nessesdesenhos animados, e analisar as implicações sociais dessas escolhas através do uso dosconceitos de dispositivo e fórmula. Além disso, a análise incluirá a retratação da cenamidiática norte-americana antes e depois de Lantz, buscando rupturas e legados.

  11. CDC Vital Signs: More People Walk to Better Health

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  12. CDC Vital Signs: Adult Smoking among People with Mental Illness

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  13. CDC Vital Signs: Colorectal Cancer Tests Save Lives

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  14. CDC Vital Signs: Prescription Painkiller Overdoses in the US

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  15. CDC Vital Signs: Getting Blood Pressure under Control

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  16. CDC Vital Signs: Adult Seat Belt Use in the US

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  17. 13 CFR 120.851 - CDC ethical requirements.

    Science.gov (United States)

    2010-01-01

    ... Company Loan Program (504) Other Cdc Requirements § 120.851 CDC ethical requirements. CDCs and their... § 120.140. In addition, they are subject to the following: (a) Any benefit flowing to a CDC's...

  18. Sistema Solar

    OpenAIRE

    Federación de Asociaciones de Astronomía Cielo de Comellas

    2004-01-01

    Lección sobre el Sistema Solar. Curso de Astronomía Básica, segunda edición, impartido por los miembros de la Federación de Asociaciones de Astronomía Cielo de Comellas. Casa de la Ciencia, sábados, del 24 de septiembre al 22 de octubre de 2011

  19. Traducción de cómic americano, superhéroes y chimichangas

    OpenAIRE

    Gonzalbes Valenzuela, Carla

    2015-01-01

    El objetivo de este trabajo es estudiar el proceso de traducción de un cómic americano. Consiste de una traducción de varios fragmentos de un cómic de Marvel de Masacre (Deadpool, en inglés) y un posterior análisis de dicha traducción, en el que se comentan los problemas que han surgido, se explica la solución al problema y se justifican las decisiones tomadas

  20. Novos táxons em Acanthocinini sul-americanos (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Marcela L. Monné

    2012-09-01

    Full Text Available Novos táxons em Acanthocinini sul-americanos (Coleoptera, Cerambycidae. São descritos e figurados os novos táxons: Trichonyssodrys nessimiani sp. nov. do Brasil (Bahia a Santa Catarina, Amniscites tavakiliani sp. nov. da Guiana Francesa and A. amboroensis sp. nov. da Bolívia (Cochabamba e Santa Cruz; Trichonius inusitatus sp. nov. e Lophopoenopsis itatiaiensis sp. nov. do Brasil (Rio de Janeiro e Luteolepturges gen. nov., espécie tipo L. galbus sp. nov., do Brasil (Amazonas, Rondônia, Peru e Equador.

  1. Primer registro de oso negro americano (Ursus americanus) para el estado de Hidalgo, México

    OpenAIRE

    Alberto Enrique Rojas-Martínez; Luis Antonio Juárez-Casillas

    2013-01-01

    Se documenta el primer registro del oso negro americano (Ursus americanus) para el estado de Hidalgo, México, mismo que representa el punto más sureño de la distribución de esta especie. El ejemplar se encontró muerto cerca del poblado El Aguaje de Cerro Prieto en un lugar conocido como La Joya, municipio de La Misión, en el norte del estado, a una altitud de 1 481 m, probablemente correspondía a un individuo errante proveniente del estado de Querétaro.

  2. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae)

    OpenAIRE

    Dilma Solange Napp; Ubirajara Ribeiro Martins

    2006-01-01

    Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae). Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae). The following new genus and species, all from Bolivia, are describ...

  3. Orquestrando vozes de gênero de estudantes estrangeiros/as latino-americanos/as

    OpenAIRE

    AlencarRodrigues, Roberta de; Strey, Marlene Neves

    2010-01-01

    Esta pesquisa discute efeitos de sentidos produzidos pelos/as estudantes estrangeiros/as latino-americanos/as acerca das concepções de gênero no país de origem e no Brasil. Fundamenta-se nos estudos de gênero e no conceito de aculturação proposto pela Psicologia Intercultural para abordar as mudanças de valores de gênero resultantes do contato com uma nova cultura. Aponta para as questões da linguagem e do discurso, ancorados na análise de discurso (AD), desenvolvida por Michel Pêcheux, utili...

  4. Dialogismo: emergência do pensamento latino-americano em comunicação

    OpenAIRE

    Yuji Gushiken

    2008-01-01

    Neste artigo estuda-se a emergência do pensamento latino-americano em comunicação, tendo como foco a proposta dialógica que, na história das teorias da comunicação, se caracteriza como crítica à mass communication research da sociologia americana. Situa a emergência desse pensamento comunicacional na América Latina em meio a condições de subdesenvolvimento econômico e social, analisando-o também como crítica aos difusionismos culturais e comunicacionais do processo de globalização. Histori...

  5. 13 CFR 120.823 - CDC Board of Directors.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false CDC Board of Directors. 120.823... Company Loan Program (504) Requirements for Cdc Certification and Operation § 120.823 CDC Board of Directors. The CDC must have a Board of Directors chosen from the membership by the members,...

  6. Fast Action Can Prevent Sepsis Death: CDC

    Science.gov (United States)

    ... fullstory_160574.html Fast Action Can Prevent Sepsis Death: CDC Know the signs of extreme response to ... treated long before it causes severe illness or death, U.S. health officials report. Sepsis, or septicemia, occurs ...

  7. CDC WONDER: Daily Fine Particulate Matter

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Daily Fine Particulate Matter data available on CDC WONDER are geographically aggregated daily measures of fine particulate matter in the outdoor air, spanning...

  8. Obesity Rates Rising Among Women: CDC

    Science.gov (United States)

    ... nlm.nih.gov/medlineplus/news/fullstory_159239.html Obesity Rates Rising Among Women: CDC Though a major ... are another group that continues to struggle with obesity. "Obesity remains a public health concern," said Cynthia ...

  9. Obesity Rates Rising Among Women: CDC

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_159239.html Obesity Rates Rising Among Women: CDC Though a major ... are another group that continues to struggle with obesity. "Obesity remains a public health concern," said Cynthia ...

  10. CDC Vital Signs: Prescription Painkiller Overdoses

    Science.gov (United States)

    ... What Can Be Done The US government is Enforcing federal laws to prevent nonmedical use of methadone. ... RSS ABOUT About CDC Jobs Funding LEGAL Policies Privacy FOIA No Fear Act OIG 1600 Clifton Road ...

  11. CDC WONDER: AIDS Public Use Data

    Data.gov (United States)

    U.S. Department of Health & Human Services — The AIDS Public Information Data Set (APIDS) for years 1981-2002 on CDC WONDER online database contains counts of AIDS (Acquired Immune Deficiency Syndrome) cases...

  12. CDC STATE System Tobacco Legislation - Licensure

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Licensure. The STATE...

  13. CDC WONDER: Mortality - Multiple Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Multiple Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2006. These data are...

  14. CDC WONDER: Sexually Transmitted Disease (STD) morbidity

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sexually Transmitted Disease (STD) Morbidity online databases on CDC WONDER contain case reports reported from the 50 United States and D.C., Puerto Rico,...

  15. CDC WONDER: Sexually Transmitted Disease (STD) Morbidity

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sexually Transmitted Disease (STD) Morbidity online databases on CDC WONDER contain case reports reported from the 50 United States and D.C., Puerto Rico,...

  16. CDC STATE System Tobacco Legislation - Preemption

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Preemption. The STATE...

  17. CDC STATE System Tobacco Legislation - Advertising

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2015. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Advertising. The STATE...

  18. CDC STATE System Tobacco Legislation - Smokefree Campus

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Campuses....

  19. CDC STATE System Tobacco Legislation - Preemption Summary

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Preemption. The STATE...

  20. CDC STATE System Tobacco Legislation - Fire Safety

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Fire-Safety. The STATE...

  1. CDC STATE System Tobacco Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Youth Access. The STATE...

  2. CDC Wonder Vaccine Adverse Event Reporting System

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Vaccine Adverse Event Reporting System (VAERS) online database on CDC WONDER provides counts and percentages of adverse event case reports after vaccination,...

  3. CDC WONDER: Mortality - Multiple Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Multiple Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2009. Data are based on...

  4. CDC WONDER: Online Tuberculosis Information System (OTIS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Online Tuberculosis Information System (OTIS) on CDC WONDER contains information on verified tuberculosis (TB) cases reported to the Centers for Disease Control...

  5. Zika Spreading Rapidly Through Puerto Rico: CDC

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_159430.html Zika Spreading Rapidly Through Puerto Rico: CDC Possibly hundreds ... 2016 FRIDAY, June 17, 2016 (HealthDay News) -- The Zika virus is spreading fast through Puerto Rico, placing ...

  6. CDC STATE System Tobacco Legislation - Tax

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation-Tax. The STATE System...

  7. CDC WONDER: Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CDC WONDER Mortality - Underlying Cause of Death online database is a county-level national mortality and population database spanning the years since 1979....

  8. CDC Vital Signs: HIV Care Saves Lives

    Science.gov (United States)

    ... your zip code to KnowIt (566948) on your smart phone to locate an HIV testing site near ... Communications (OADC) Email Recommend Tweet YouTube Instagram Listen Watch RSS ABOUT About CDC Jobs Funding LEGAL Policies ...

  9. Léxico asociado a los recursos naturales americanos en el español de Canarias

    OpenAIRE

    Cáceres-Lorenzo, María Teresa; Salas-Pascual, Marcos

    2010-01-01

    International audience La formación del léxico peculiar de una región está íntimamente ligado a las circunstancias históricas, económicas y sociales del mismo. La relación entre los territorios americanos y las Islas Canarias se inicia desde el mismo momento del descubrimiento hasta nuestros días. Esta relación puede ejemplificarse de manera perfecta en el vocabulario vinculado a los recursos naturales de origen americano que han determinado la agricultura, parte de la industria, e incluso...

  10. [Sistema Educativo

    OpenAIRE

    Silva, Bento Duarte da

    1989-01-01

    A consagração na Lei nº 46/86, mais conhecida por Lei de Bases do Sistema Educativo (LBSE), aprovada em Outubro de 1986, da criação dos departamentos regionais de educação (44º - 2) que podem vir a reunir um amplo leque de competências em todos os sectores educativos , de estruturas a nível local que assegurem a interligação do sistema educativo com a comunidade (43º - 2) atribuindo-se especial responsabilidade aos municipios (63º - 2), só por si justifica e torna premente a realização deste ...

  11. Modernismo Latino-Americano e construção de identidades através da pintura

    Directory of Open Access Journals (Sweden)

    Maria Helena Rolim Capelato

    2005-12-01

    Full Text Available O texto tem como objetivo refletir sobre as representações visuais expressas em algumas obras de artistas plásticos latino-americanos, vinculados aos movimentos modernistas dos anos 1920 que, através da pintura, procuraram traduzir suas preocupações com a busca de identidades nacionais ou regionais.

  12. CDC Climat - 2011 Sustainable Development Report

    International Nuclear Information System (INIS)

    CDC Climat is the Caisse des Depots (CDC) subsidiary that is dedicated to combating climate change. Its activities aim to support the transition towards a low resource and low greenhouse gas emission (GHG) economy, through services that are cutting-edge, pro table, and in line with CDC's public policy goals. Through its corporate purpose, CDC Climat embodies the CDC's commitments in the sustainable development field. CDC Climat supports the implementation of public GHG emission reduction policies, primarily through emission trading schemes at the European and international level. Since it was founded in 2010, and throughout 2011, its strategic priorities have consisted in: - developing a long-term policy for investing in carbon credits generated by environmental initiatives, as part of the project mechanisms set up by the Kyoto Protocol, and used in the European Emission Trading Scheme; - supporting the development of its investments in carbon finance operators, like BlueNext, the European carbon exchange, for instance; - broadening the scope of its research into climate economics, which is supported by CDC and available to everyone, in order to serve the public and private players concerned. Its teams have supported French and European governments, international organisations and the United Nations, and various NGOs in their work and thinking on the future of tools for combating climate change. They have specifically contributed reports based on their research and operational feedback. When it was founded, CDC Climat was closely linked to public policies aimed at combating climate change via allowance and carbon trading mechanisms. The difficulties encountered by international negotiations, together with the effects of the economic and financial downturn in Europe, have resulted in a very pronounced fall in the price of carbon assets on these markets since the summer of 2011, with no prospect of recovery for several years. This environment is calling some of the

  13. Criterios para un sistema de evaluación de la calidad educativa en Guaviare

    OpenAIRE

    Arias Rodríguez, Julio César

    2013-01-01

    Definir unos criterios que nos permitan acercarnos a la construcción de un sistema de evaluación de la calidad de la educación para el departamento del Guaviare, implica reconocer la evaluación como una acción social que ha estado presente en la historia de la humanidad; ella no nació en el campo de la educación sino en el campo de la industria, y a partir de la década de 1990 es asumida en el contexto Latino Americano como una exigencia de las agencias internacionales (Banco Mundial, Fondo M...

  14. Sistemas agroalimentarios localizados. Innovación y debates desde América Latina

    OpenAIRE

    Gerardo Torres Salcido

    2013-01-01

    http://dx.doi.org/10.5007/1807-1384.2013v10n2p68Este artigo propõe uma reflexão sobre os problemas enfrentados pelo planejamento do desenvolvimento local e sobre as críticas centradas na localização espacial das atividades econômicas, explorando as implicações desse argumento para o estudo de sistemas agroalimentares localizados (SIAL). Além disso, o autor avalia de que maneira as pesquisas conduzidas em diversos países latino-americanos vêm contribuindo para a maturação progressiva do enfoqu...

  15. The role of CDC42 in cancer%CDC42与癌症

    Institute of Scientific and Technical Information of China (English)

    徐永茹; 徐平; 徐锋; 李湘萍

    2016-01-01

    Cell division cycle 42 (CDC42)is a member of Rho guanosine triphosphatase (GTPase) family,which plays an important role in cell proliferation , cell migration and cell transformation .The activity of CDC42 can be regulated by guanine nucleotide exchange factors (GEFs),GTPase activating proteins (GAPs) and guanine nucleotide-dissociation inhib-itors (GDIs).Recently,CDC42 has been reported as abnormally expressed in many human cancers ,suggesting that CDC42 performs complex functions in tumorigenesis .Therefore,this paper aims to shed light on the functions of CDC 42 in cancers in terms of the alteration of CDC42 activity,CDC42 regulators,as well as its effectors.%细胞分裂周期蛋白42(cell division cycle 42,CDC42)是Rho蛋白鸟苷三磷酸酶(guanosine triphosphatase, GTPase)家族成员之一,在细胞增殖、迁移、转化等过程发挥重要作用。与大多数GTPase一样,CDC42的活性受到鸟嘌呤核苷酸转换因子( guanine nucleotide exchange factors , GEF )、GTP 酶激活蛋白( GTPase activating proteins , GAP)和鸟苷酸解离抑制因子( guanine nucleotide-dissociation inhibitors ,GDI)的调控。目前研究表明,CDC42在大多数人癌症组织中表达异常,对癌症的发生发展具有复杂而重要的调控作用。该文就CDC42自身活性变化、CDC42调节因子的变化及其下游效应因子的变化在癌症中的作用进行综述,旨在深入理解CDC42在人类癌症中的作用机制。

  16. A censura aos filmes ibero-americanos na governação de Marcello Caetano

    OpenAIRE

    Morais, Ana Bela

    2014-01-01

    Através do estudo dos processos de censura ao filmes hispano-americanos em Portugal durante os anos de governação de Marcello Caetano (finais de 1968-1974), pretendo investigar os critérios da Comissão de Censura em relação ao modo como eram censurados esses filmes. O presente trabalho apoia-se no estudo dos arquivos do Secretariado Nacional da Informação e Turismo. A informação produzida pela Comissão de Exame e Classificação de Espectáculos, durante o Estado Novo, está concentrada neste esp...

  17. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Dilma Solange Napp

    2006-01-01

    Full Text Available Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae. Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae. The following new genus and species, all from Bolivia, are described in Compsocerini: Ecoporanga gen. nov., type species E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., from Santa Cruz, and Ethemon iuba sp. nov. from Cochabamba.

  18. Conflitos sobre o conflito: crise colombo-equatoriana em jornais latino- americanos

    Directory of Open Access Journals (Sweden)

    Angela Zamin

    2013-07-01

    Full Text Available O artigo analisa a produção jornalística do acontecimento ‘Angostura’, no período de março de 2008 a agosto de 2009, a partir dos jornais latino-americanos O Estado de S. Paulo, El Tiempo e El Comercio. No nível discursivo, orienta-se por três modos de aproximação entre Jornalismo e conflito: (1 conflitos que o Jornalismo reconhece; (2 conflitos que provoca, e (3 que o envolvem. Percebe-se, deste modo, o papel do Jornalismo na construção das estruturas do conflito e na circulação dele.

  19. Cdc42-mediated tubulogenesis controls cell specification

    DEFF Research Database (Denmark)

    Kesavan, Gokul; Sand, Fredrik Wolfhagen; Greiner, Thomas Uwe;

    2009-01-01

    Understanding how cells polarize and coordinate tubulogenesis during organ formation is a central question in biology. Tubulogenesis often coincides with cell-lineage specification during organ development. Hence, an elementary question is whether these two processes are independently controlled...... later for maintaining apical cell polarity. Finally, we show that Cdc42 controls cell specification non-cell-autonomously by providing the correct microenvironment for proper control of cell-fate choices of multipotent progenitors. For a video summary of this article, see the PaperFlick file with the......, or whether proper cell specification depends on formation of tubes. To address these fundamental questions, we have studied the functional role of Cdc42 in pancreatic tubulogenesis. We present evidence that Cdc42 is essential for tube formation, specifically for initiating microlumen formation and...

  20. January 21, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-01-21

    The latest numbers from CDC show more than 20,000 people in West Africa have been sick with Ebola, and more than 8,000 have died. As Dr. Tom Frieden explains, some areas may be starting to see a decrease in cases, but that does not mean the fight is over. CDC will continue its work in West Africa until we get to zero new cases.  Created: 1/21/2015 by CDC’s Office of the Associate Director for Communication.   Date Released: 1/21/2015.

  1. CDC Best Practices for Comprehensive Tobacco Control Programs - 2007

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). Best Practices for Comprehensive Tobacco Control Programs. Funding. CDC's Best Practices for Comprehensive Tobacco...

  2. CDC Best Practices for Comprehensive Tobacco Control Programs - 2014

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). Best Practices for Comprehensive Tobacco Control Programs. Funding. CDC's Best Practices for Comprehensive Tobacco...

  3. Characterization and Expression of Mouse Cdc50c during Spermatogenesis

    Institute of Scientific and Technical Information of China (English)

    Peng XU; Xiaoyan DING

    2007-01-01

    Cdc50p is a transmembrane protein required for polarized growth in Saccharomyces cerevisiae.The knowledge on physiological functions of its mammalian homologs, however, is limited. Using database analysis, we identified one mouse testis expressed sequence tag, named Cdc50c, encoding a previously uncharacterized homolog of Cdc50p. Similar to yeast Cdc50p, the putative Cdc50c protein contains three transmembrane spanning regions. Its orthologs are present in many species such as fish, avian and human,suggesting its evolutionary conservation. In multitissue reverse transcription-polymerase chain reaction analyses the mRNA for Cdc50c was predominately detected in testis. The onset of the gene expression coincides with the first appearance of spermatocytes during testicular development. In situ hybridization analyses revealed that Cdc50c mRNA localized in pachytene spermatocytes and round and elongated spermatids. Our data suggest that Cdc50c might play important roles during spermatogenesis.

  4. June 2, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-06-02

    In this podcast, CDC's Dr. Jane Seward discusses the Ebola vaccine trial known as STRIVE, which began in April 2015.  Created: 6/2/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 6/2/2015.

  5. December 17, 2014 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2014-12-17

    This podcast provides an update on the Ebola response, as of December 17, 2014. It briefly describes CDC's "Operation Care Package.".  Created: 12/17/2014 by CDC’s Office of the Associate Director for Communication.   Date Released: 12/17/2014.

  6. Un puente entre los mundos. El Instituto Ibero-Americano de Berlín

    Directory of Open Access Journals (Sweden)

    Diana von Römer

    2005-10-01

    Full Text Available El presente artículo ofrece al lector una visión de conjunto sobre el Instituto Ibero-Americano (IAI en Berlín. En primer lugar, relata la historia del instituto desde que se fundara en 1930, cuando surgió el modelo de tres pilares: información, investigación e intercambio cultural, hasta la actualidad, en la que éste sigue vigente. A continuación se exponen en detalle las tres funciones principales que cumple el instituto: centro de información - el IAI alberga la mayor biblioteca de Europa especializada en las culturas ibero-americanas - con sus diversos fondos y colecciones; centro de investigación, con el apoyo a la investigación a través de becas, publicaciones y coloquios; y finalmente el centro cultural, con actividades de diversa índole en colaboración con otras instituciones y embajadas, favoreciendo así el diálogo entre Alemania e Iberoamérica, el Caribe, España y Portugal.

  7. ECIS - Adaptation to CDC CYBER system

    International Nuclear Information System (INIS)

    The implantation of ECIS computer code, elaborated for BOURROUGHS 6700 computer of USP (Universidade de Sao Paulo), in the CDC CYBER 170/730 computer is presented. The ECIS code calculates cross section by coupled channel method. Some problems were calculated to verify the compactibility of results obtained from BOURROUGHS 6700 version with the version. The problems calculated by new version and the carried out modifications, are described (M.C.K.)

  8. February 20, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-02-20

    A strategy to rapidly identify and respond to cases of Ebola in remote areas in Liberia led to a drastic reduction in Ebola cases and cut the duration of these cluster outbreaks in half. Learn how CDC responders and their partners trekked through the jungle in their efforts to quickly stop the spread of the virus.  Created: 2/20/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 2/20/2015.

  9. CDC Vital Signs-Preventing Melanoma

    Centers for Disease Control (CDC) Podcasts

    2015-06-02

    This podcast is based on the June 2015 CDC Vital Signs report. Skin cancer is the most common form of cancer in the U.S. In 2011, there were more than 65,000 cases of melanoma, the most deadly form of skin cancer. Learn how everyone can help prevent skin cancer.  Created: 6/2/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 6/2/2015.

  10. CDC Vital Signs-Heroin Epidemic

    Centers for Disease Control (CDC) Podcasts

    2015-07-07

    This podcast is based on the July 2015 CDC Vital Signs report. Heroin use and heroin-related overdose deaths are increasing. Most people are using it with other drugs, especially prescription opioid painkillers. Learn what can be done to prevent and treat the problem.  Created: 7/7/2015 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 7/7/2015.

  11. September 24, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-09-24

    In this podcast, CDC medical officer Alyson Goodman describes how CDC’s Children’s Health Team worked with partners to help U.S. hospitals prepare for a potential case of Ebola in a child.  Created: 9/24/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 9/24/2015.

  12. El imaginario flamenco americano: Aura y Kitsch en la escena transnacional

    Directory of Open Access Journals (Sweden)

    Asensio, Susana

    2004-12-01

    Full Text Available The continuous transformations suffered by flamenco have always been related to its interactions with many other traditions. What once was a local experience, although hybrid from its origins, was transformed into a chameleonic musical phenomenon able to produce new development while conserving a characteristic personality. The transnational flamenco progress started around the end of 19th century. It is considered that its trips to America, especially those to the United States, were the most relevant to the phenomenon. However, flamenco artists developed their careers detached from its socio-cultural environment —and even from American cultures— in the new settings. Their isolation was twofold and favored specific ways to perform and experience flamenco. This new expansion gave transnational flamenco a distinctive and contemporary Kitsch touch.

    Las continuas transformaciones sufridas por el flamenco han estado siempre relacionadas con su interacción con innumerables tradiciones. Lo que una vez fue un fenómeno local, aunque híbrido desde su origen, se convierte en un fenómeno musical camaleónico, capaz de producir fenómenos nuevos al tiempo que sigue manteniendo su personalidad distintiva. El movimiento transnacional del flamenco comenzó ya en el siglo XIX, aunque podemos considerar que su viaje a los Estados Unidos es el que más relevancia ha tenido. Allí, sin embargo, los artistas desarrollaron sus carreras no sólo aislados del entorno sociocultural flamenco, sino también de la cultura popular americana. Su aislamiento fue doble y así los artistas americanos desarrollaron maneras específicas de interpretar y experienciar el flamenco representativas del Kitsch más contemporáneo.

  13. Dialogismo: emergência do pensamento latino-americano em comunicação

    Directory of Open Access Journals (Sweden)

    Yuji Gushiken

    2008-09-01

    Full Text Available Neste artigo estuda-se a emergência do pensamento latino-americano em comunicação, tendo como foco a proposta dialógica que, na história das teorias da comunicação, se caracteriza como crítica à mass communication research da sociologia americana. Situa a emergência desse pensamento comunicacional na América Latina em meio a condições de subdesenvolvimento econômico e social, analisando-o também como crítica aos difusionismos culturais e comunicacionais do processo de globalização. Historicamente, o dialogismo atualiza os processos de inovação teórica que reavaliaram a recepção como mediadora e produtora de sentidos nos estudos em comunicação. Palavras-chave: Comunicação; dialogismo; América Latina. ABSTRACT In this article, latin-american thought emergence in communication is studied focusing on the dialogue proposal which, in the history of communication theories, is characterised as a critique of mass communication research in American sociology. Latin-american thought emergence in communication is situated within conditions of economic and socialunderdevelopment being analysed also as a critique of the idea of cultural and communication dispersion of the globalisation process. Historically, dialogism updates the processes of theory innovation which reassesses reception as a mediator and producer of meaning in the studies of communication. Keywords: Communication; dialogism; Latin America.

  14. Analysis list: Cdc73 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Cdc73 Muscle + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Cdc73.1.ts...v http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Cdc73.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Cd...c73.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Cdc73.Muscle.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Muscle.gml ...

  15. Presencia de visón americano (Neovison vison, Schreber 1777 en Chiloé, Chile: ¿inicio de una invasión biológica?

    Directory of Open Access Journals (Sweden)

    G. Vergara

    2015-01-01

    Full Text Available El visón americano fue introducido en Chile en la década de 1930 y actualmente presenta una amplia distribución geográfica. Sin embargo, no se han reportado poblaciones establecidas en muchos sistemas insulares. El día 6 de febrero de 2013, fue hallado un individuo juvenil en Chacao, Chiloé, Chile. El Canal de Chacao representa la única barrera biogeográfica a la isla de Chiloé, con una distancia mínima al continente de 2 km. Se presume que el individuo sorteó esta barrera por sus propios medios, ingresó en una embarcación o mediante un tercero. La introducción de un mamífero carnívoro podría generar severas consecuencias para los ecosistemas de la isla. Los planes preventivos con la comunidad e instituciones públicas y privadas representan una herramienta esencial para el manejo de esta especie y evitar tales impactos en Chiloé.

  16. CDC Vital Signs-Hispanic Health

    Centers for Disease Control (CDC) Podcasts

    2015-05-05

    This podcast is based on the May 2015 CDC Vital Signs report. About one in six people living in the U.S. are Hispanic. The two leading causes of death in this group are heart disease and cancer, accounting for two out of five deaths. Unfortunately, many Hispanics face considerable barriers to getting high quality health care, including language and low income. Learn what can be done to reduce the barriers.  Created: 5/5/2015 by Office of Minority Health & Health Equity (OMHHE).   Date Released: 5/5/2015.

  17. April 28, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-04-28

    In any disease outbreak, misinformation, a lack of understanding, and fear can lead to unfortunate side effects, like stigma. Stigma presents a challenge for communities during a time when they need to be strong to fight the disease. In this podcast, Molly Gaines-McCollom, CDC Health Communication Specialist, discusses the impact of stigma in the current Ebola outbreak and why it’s so important to fight it.  Created: 4/28/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 4/28/2015.

  18. CDC Vital Signs-Heart Age

    Centers for Disease Control (CDC) Podcasts

    2015-09-01

    This podcast is based on the September 2015 CDC Vital Signs report. Your heart age is the age of your heart and blood vessels as a result of your risk factors for heart attack and stroke. If you smoke or have high blood pressure, your heart age will be much higher than your actual age. Learn what you can do to lower your heart age and keep it low.  Created: 9/1/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/1/2015.

  19. March 19, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-03-19

    The Ebola outbreak has caused many people to ask whether animals, or specifically pets, are at risk of getting and spreading Ebola in the United States. In this podcast, Drs. Casey Barton Behravesh and Heather Bair-Brake, veterinarians at CDC, discuss how Ebola can affect animals, whether pets in the U.S. are at risk, and what people being monitored for Ebola should do if they have pets at home.  Created: 3/19/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 3/19/2015.

  20. January 8, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-01-08

    CDC scientists, recently returned from Guinea, recount their infection control training course for Guinean healthcare workers who work in health facilities other than Ebola Treatment Units. These workers treat anyone from pregnant women to suspected Ebola cases. It’s critical they be able to recognize and properly treat Ebola patients, not only to protect their own health, but that of their patients.  Created: 1/8/2015 by CDC’s Office of the Associate Director for Communication.   Date Released: 1/8/2015.

  1. Perseguir al SRAG: CDC en acción (Stalking SARS: CDC at Work)

    Centers for Disease Control (CDC) Podcasts

    2013-04-29

    En este podcast los niños de Kidtastics hablan sobre el brote del SRAS y cómo trabajaron los CDC para resolver el misterio.  Created: 4/29/2013 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 8/10/2016.

  2. O DIREITO FUNDAMENTAL AO MEIO AMBIENTE NO NOVO CONSTITUCIONALISMO LATINO-AMERICANO: BOLÍVIA E EQUADOR

    Directory of Open Access Journals (Sweden)

    Ana Cristina Willemann

    2013-11-01

    constitucional que vem se desenvolvendo nos países latinos; e (ii abordar o direito ao meio ambiente na nova visão do constitucionalismo latino-americano; e analisar como o respeito a Pacha Mama, Madre Tierra, impõe o direito fundamental à água e aos outros seres vivos. A metodologia utilizada é a revisão bibliográfica por meio de leitura e análise comparada dos textos dos autores mencionados na bibliografia deste artigo.

  3. Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes

    International Nuclear Information System (INIS)

    CDC25 phosphatases are important regulators of the cell cycle. Their abnormal expression detected in a number of tumors implies that their dysregulation is involved in malignant transformation. However, the role of CDC25s in vulvar cancer is still unknown. To shed light on their roles in the pathogenesis and to clarify their prognostic values, expression of CDC25A, CDC25B and CDC25C in a large series of vulvar squamous cell carcinomas were examined. Expression of CDC25A, CDC25B, CDC25C and phosphorylated (phospho)-CDC25C (Ser216) were examined in 300 vulvar carcinomas using immunohistochemistry. Western blot analysis was utilized to demonstrate CDC25s expression in vulvar cancer cell lines. Kinase and phosphatase assays were performed to exclude cross reactivity among CDC25s isoform antibodies. High nuclear CDC25A and CDC25B expression were observed in 51% and 16% of the vulvar carcinomas, respectively, whereas high cytoplasmic CDC25C expression was seen in 63% of the cases. In cytoplasm, nucleus and cytoplasm/nucleus high phospho-CDC25C (Ser216) expression was identified in 50%, 70% and 77% of the carcinomas, respectively. High expression of CDC25s correlated significantly with malignant features, including poor differentiation and infiltration of vessel for CDC25B, high FIGO stage, presence of lymph node metastases, large tumor diameter, poor differentiation for CDC25C and high FIGO stage, large tumor diameter, deep invasion and poor differentiation for phospho-CDC25C (Ser216). In univariate analysis, high expression of phospho-CDC25C (Ser216) was correlated with poor disease-specific survival (p = 0.04). However, such an association was annulled in multivariate analysis. Our results suggest that CDC25C and phospho-CDC25C (Ser216) play a crucial role and CDC25B a minor role in the pathogenesis and/or progression of vulvar carcinomas. CDC25B, CDC25C and phospho-CDC25C (Ser216) were associated with malignant features and aggressive cancer phenotypes. However, the

  4. SISTEMA INTERAMERICANO, COOPERAÇÃO E A COMPETIÇÃO INTRAREGIONAL

    Directory of Open Access Journals (Sweden)

    Sabrina Evangelista Medeiros

    2014-12-01

    Full Text Available Este texto tem como propósito a análise do sistema inter-americano e da Organização dos Estados Americanos por meio de sua história e componentes relacionadas às tratativas e organicidade do sistema. Para isso, esta abordagem tem como base a sua evolução institucional, os diferentes usos conceituais e de propósito, as principais fases deste sistema e, em consequência, a reputação associada a ele em que pese os marcos regulatórios e compromissos assumidos no âmbito da OEA e fora dele. A hipótese é de que, a OEA teve constante evolução institucional por conta da transição de objetivos que sofreu entre o fim da Guerra Fria e o século XXI e que, nesta conjuntura e como forma de adaptar-se às outras frentes regionais potencialmente competitivas, volta-se para a questão da segurança individual e direitos humanos criando um guarda-chuva em que cabem, de maneira complementar, as demais iniciativas setorialmente organizadas, como as de finalidade energética, comercial ou de defesa. Por último, que a configuração do setor de defesa nestes mesmos marcos regulatórios criados ao longo destes anos apresenta-se pela fusão de fatores multidimensionais, que vão desde a segurança civil às fronteiras, com poucos aspectos tradicionais de defesa, realocando o seu papel na região

  5. CDC Vital Signs: Making Health Care Safer -- Protect Patients from Antibiotic Resistance

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  6. CDC Vital Signs: Heart Age - Is Your Heart Older Than You?

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  7. CDC Vital Signs: Making Health Care Safer -- Stop Infections from Lethal CRE Germs Now

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Vital Signs Note: Javascript is disabled or is not supported ... message, please visit this page: About CDC.gov . Vital Signs Current issue Infographic Topics Covered Alcohol Cancer Cardiovascular ...

  8. Desenvolvimento de Sistemas Gastroretentivos

    OpenAIRE

    Bettencourt, Catarina Maria Silveira

    2012-01-01

    Trabalho apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas Os sistemas gastroretentivos são formas farmacêuticas de libertação modificada desenhadas com o intuito de prolongarem o seu tempo de residência no trato gastrointestinal superior. Estes sistemas surgiram para preencher lacunas apresentadas pelos sistemas convencionais de libertação modificada. Os sistemas de libertação controlada convencionais não são des...

  9. Um exemplo de inversão do domínio ideológico norte-americano no Brasil

    OpenAIRE

    Carla Damião

    2014-01-01

    Propomos discutir a relação de domínio ideológico-cultural norte-americano sobre o Brasil, tendo em vista exemplos documentados. Trata-se da chamada “política de boa vizinhança” [Good Neighbor Policy], com início em 1933 até o final da Segunda Guerra, notadamente durante o governo Getúlio Vargas no Brasil e o governo Franklin Roosevelt nos Estados Unidos. A necessidade de propaganda política passava pela “sedução” cultural da potência estadunidense sobre seus vizinhos latino-americanos. Neste...

  10. A América dos 33: a proposta de criação da Comunidade de Estados Latino-Americanos e Caribenhos (CELAC

    Directory of Open Access Journals (Sweden)

    Pedro Ernesto Fagundes

    2010-03-01

    Full Text Available

    Esse artigo analisa proposta de criação da Comunidade de Estados Latino-Americanos e Caribenhos (CELAC, organização que substituiria a Organização dos Estados Americanos (OEA.

     

  11. Roles of the CDK Phosphorylation Sites of Yeast Cdc6 in Chromatin Binding and Rereplication

    OpenAIRE

    Honey, Sangeet; Futcher, Bruce

    2007-01-01

    The Saccharomyces cerevisiae Cdc6 protein is crucial for DNA replication. In the absence of cyclin-dependent kinase (CDK) activity, Cdc6 binds to replication origins, and loads Mcm proteins. In the presence of CDK activity, Cdc6 does not bind to origins, and this helps prevent rereplication. CDK activity affects Cdc6 function by multiple mechanisms: CDK activity affects transcription of CDC6, degradation of Cdc6, nuclear import of Cdc6, and binding of Cdc6 to Clb2. Here we examine some of the...

  12. El Sistema's Open Secrets

    Science.gov (United States)

    Booth, Eric

    2011-01-01

    In this article, the author talks about Venezuela's national youth orchestra program called El Sistema whose attributes offer a direct challenge to traditional Western music practices. As U.S. classical music--and all "high arts"--struggle to find relevance to more than the small "arts club" percentage of the U.S. populace, El Sistema proposes…

  13. March 11, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-03-11

    CDC’s Eric Dziuban explains key messages CDC developed with UNICEF and the World Health Organization (WHO) on safe school operations in Guinea, Liberia, and Sierra Leone. After being closed for months due to the Ebola outbreak, schools in these countries are reopening – an important step in helping children and their communities return to normal. The key messages offer a tool that each country’s government can use to develop their own approach on safely reopening schools.  Created: 3/11/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 3/11/2015.

  14. Estudos latino-americanos sobre melancolia: um transtorno do humor melhor definido para o CID-11 Melancholia in Latin American studies: a distinct mood disorder for the ICD-11

    Directory of Open Access Journals (Sweden)

    Mario F. Juruena

    2011-05-01

    Full Text Available OBJETIVO: A depressão melancólica é um diagnóstico psiquiátrico de história de vida, geralmente com episódios recorrentes. Melancolia é uma síndrome com longa duração e características específicas de psicopatologia, insuficientemente diferenciada de depressão maior por um especificador no DSM-IV e parcialmente descrito nos critérios da Classificação Internacional de Doenças-10ª Edição. Dentro da classificação atual, é frequentemente vista em pacientes gravemente doentes com depressão e transtorno bipolar. No entanto, a melancolia possui uma homogeneidade psicopatológica e biológica distinta na experiência clínica e nos marcadores de testes laboratoriais, e é diferencialmente sensível às intervenções terapêuticas específicas. O objetivo deste estudo é revisar a literatura de artigos publicados por autores latino-americanos sobre a melancolia. MÉTODO: Realizou-se busca de artigos latino-americanos de informações relevantes para a revisão da Classificação Internacional de Doenças-10ª Edição de transtornos mentais e comportamentais em pacientes com depressão melancólica. Foi avaliada a qualidade do design de todos os estudos e realizada uma revisão abrangente sobre o assunto, com o objetivo de considerar a contribuição latino-americana para inclusão da melancolia como uma entidade distinta na futura Classificação Internacional de Doenças-11ª Edição. RESULTADOS E CONCLUSÃO: Os estudos latino-americanos fundamentam o diagnóstico da melancolia com uma psicopatologia e psiconeuroendocrinologia própria que fundamentam ser reconhecida como um transtorno de humor identificável e merecedor de uma atenção específica nos sistemas de classificação, como um transtorno de humor distinto, identificável e especificamente tratável.OBJECTIVE: Melancholic depression is a lifetime diagnosis, typically with recurrent episodes. Melancholia, a syndrome with a long history and distinctive

  15. Flavors of the States ‘Saborea los estados’. Proyecto web para la gestión y venta de productos alimenticios americanos

    OpenAIRE

    Aguado García, Carlos

    2013-01-01

    Flavors of the States “Saborea los estados” es un proyecto web para la gestión y venta de productos alimenticios americanos. Es un proyecto en plataforma.net y en un lenguaje C#, utilizando SQL Server e incorporando una pasarela de pago PayPal

  16. Sistemas agroalimentarios localizados. Innovación y debates desde América Latina

    Directory of Open Access Journals (Sweden)

    Gerardo Torres Salcido

    2013-12-01

    Full Text Available http://dx.doi.org/10.5007/1807-1384.2013v10n2p68Este artigo propõe uma reflexão sobre os problemas enfrentados pelo planejamento do desenvolvimento local e sobre as críticas centradas na localização espacial das atividades econômicas, explorando as implicações desse argumento para o estudo de sistemas agroalimentares localizados (SIAL. Além disso, o autor avalia de que maneira as pesquisas conduzidas em diversos países latino-americanos vêm contribuindo para a maturação progressiva do enfoque SIAL. Para tanto, adota uma perspectiva ética que resgata a visão humanista sobre a problemática da segurança alimentar no atual contexto de globalização econômico-financeira e cultural.

  17. Optimizacija SCR sistema

    OpenAIRE

    Berić, Sašo

    2013-01-01

    Računalniška dinamika tekočin predstavlja učinkovito orodje pri iskanju izboljšav na področju izpušnih sistemov kot je SCR (angl.: Selectiv Catalytic Reduction, Selektivna katalitična redukcija) sistem. Omogoča analiziranje tokovnih karakteristik znotraj izpušnega sistema, vpliva geometrije sistema na konverzijo dušikovih oksidov. V predstavljeni nalogi je prikazano iterativno optimiranje SCR sistema s pomočjo računalniške dinamike tekočin. Najprej je bila izvedena numerična analiza obstoj...

  18. O marxismo indo-americano de Mariátegui e a polêmica com a III Internacional Comunista.

    Directory of Open Access Journals (Sweden)

    María Gabriela Guillén

    2012-05-01

    Full Text Available Partindo da perspectiva marxista indo-americana de José Carlos Mariátegui, o artigo visa a exposição de fatores teóricos e históricos que culminaram na polêmica com a III Internacional Comunista na América Latina. A problemática evidencia a originalidade do pensamento de Mariátegui, cujo eixo central é a interpretação dos fatos históricos, políticos e sociais latino-americanos sob a perspectiva dialética do desenvolvimento desigual e combinado para explicar a dinâmica particular da realidade peruana.

  19. Poder civil y derechos naturales de los indios americanos según Fray Alonso de la Veracruz

    Directory of Open Access Journals (Sweden)

    Manuel Méndez Alonzo

    2013-01-01

    Full Text Available El presente trabajo investiga las tesis sobre el poder civil de Alonso de la Veracruz que buscan incorporar en la comunidad política española a los habitantes autóctonos del Nuevo Mundo, tesis que suelen relacionarse con F. de Vitoria y el tomismo español, y que últimamente son consideradas parte del republicanismo novohispano elaborado desde la periferia americana. Se busca demostrar que su propósito era aplicar una teoría de derechos naturales, sin que ello implique participación política de los indios americanos. Se analiza la postura del fraile frente a la diversidad cultural y la guerra contra los indios.

  20. 13 CFR 120.857 - Voluntary transfer and surrender of CDC certification.

    Science.gov (United States)

    2010-01-01

    ... of CDC certification. 120.857 Section 120.857 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Other Cdc Requirements § 120.857 Voluntary transfer and surrender of CDC certification. A CDC may not transfer its certification or withdraw from...

  1. The Role of Cdc2 and Other Genes in Meiosis in Schizosaccharomyces Pombe

    OpenAIRE

    Iino, Y.; Hiramine, Y.; Yamamoto, M.

    1995-01-01

    The requirement of the cdc2, cdc13 and cdc25 genes for meiosis in Schizosaccharomyces pombe was investigated using three different conditions to induce meiosis. These genes were known to be required for meiosis II. cdc13 and cdc25 are essential for meiosis I. The cdc2 gene, which is required for the initiation of both mitotic S-phase and M-phase, is essential for premeiotic DNA synthesis and meiosis II. The requirement of cdc2 for meiosis I was unclear. This contrasts with Saccharomyces cerev...

  2. Zika Virus Causes Brain Defects in Babies: CDC

    Science.gov (United States)

    ... nih.gov/medlineplus/news/fullstory_158287.html Zika Virus Causes Brain Defects in Babies: CDC Agency says ... 2016 WEDNESDAY, April 13, 2016 (HealthDay News) -- Zika virus is a definite and direct cause of microcephaly ...

  3. Tuberculosis Decline in U.S. Has Stalled, CDC Reports

    Science.gov (United States)

    ... nlm.nih.gov/medlineplus/news/fullstory_157951.html Tuberculosis Decline in U.S. Has Stalled, CDC Reports Agency ... HealthDay News) -- Two decades of progress toward eliminating tuberculosis in the United States has stalled, with incidence ...

  4. Cdc42 promotes host defenses against fatal infection

    DEFF Research Database (Denmark)

    Lee, Keunwook; Boyd, Kelli L; Parekh, Diptiben V;

    2013-01-01

    The small Rho GTPase, Cdc42, regulates key signaling pathways required for multiple cell functions including maintenance of shape, polarity, proliferation, invasion, migration, differentiation and morphogenesis. As the role of Cdc42-dependent signaling in fibroblasts in vivo is unknown, we...... attempted to specifically delete it in these cells by crossing the Cdc42(fl/fl) mouse with a FSP-1 cre mouse, which is thought to mediate recombination exclusively in fibroblasts. Surprisingly, the FSP-1cre;Cdc42(fl/fl) mice died at 3 weeks of age due to overwhelming suppurative upper airway infections that...... were associated with neutrophilia and lymphopenia. Even though major aberrations in lymphoid tissue development were present in the mice, the principal cause of death was severe migration and killing abnormalities of the neutrophil population resulting in an inability to control infection. We also...

  5. CDC STATE System E-Cigarette Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Youth...

  6. Southern States Lagging in Tough Smoking Bans, CDC Says

    Science.gov (United States)

    ... 159529.html Southern States Lagging in Tough Smoking Bans, CDC Says Only 6 in 10 Americans covered ... federal government report says. This type of law bans smoking in all indoor areas of workplaces, restaurants ...

  7. CDC Warns of Dangers of Plastic Surgery in Dominican Republic

    Science.gov (United States)

    ... a very mutilating infection. They're going for cosmetic surgery, and they will be scarred. It's a terrible ... postoperative infections, particularly ones that are related to cosmetic surgery," Daley said. The CDC report warns about the ...

  8. CDC STATE System E-Cigarette Legislation - Preemption

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Preemption....

  9. CDC STATE System E-Cigarette Legislation - Tax

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Tax. The...

  10. CDC STATE System E-Cigarette Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  11. CDC STATE System Tobacco Legislation - Smokefree Indoor Air Summary

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Indoor Air....

  12. CDC STATE System E-Cigarette Legislation - Smokefree Campus

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  13. CDC STATE System E-Cigarette Legislation - Licensure

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Licensure....

  14. CDC STATE System Tobacco Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Indoor Air....

  15. CDC WONDER: Vaccine Adverse Event Reporting System (VAERS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Vaccine Adverse Event Reporting System (VAERS) online database on CDC WONDER provides counts and percentages of adverse event case reports after vaccination, by...

  16. CDC WONDER: Compressed Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CDC WONDER Mortality - Underlying Cause of Death online database is a county-level national mortality and population database spanning the years since 1979...

  17. Cell cycle sibling rivalry: Cdc2 vs. Cdk2.

    Science.gov (United States)

    Kaldis, Philipp; Aleem, Eiman

    2005-11-01

    It has been long believed that the cyclin-dependent kinase 2 (Cdk2) binds to cyclin E or cyclin A and exclusively promotes the G1/S phase transition and that Cdc2/cyclin B complexes play a major role in mitosis. We now provide evidence that Cdc2 binds to cyclin E (in addition to cyclin A and B) and is able to promote the G1/S transition. This new concept indicates that both Cdk2 and/or Cdc2 can drive cells through G1/S phase in parallel. In this review we discuss the classic cell cycle model and how results from knockout mice provide new evidence that refute this model. We focus on the roles of Cdc2 and p27 in regulating the mammalian cell cycle and propose a new model for cell cycle regulation that accommodates these novel findings. PMID:16258277

  18. CDC WONDER: Daily Air Temperatures and Heat Index

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Daily Air Temperature and Heat Index data available on CDC WONDER are county-level daily average air temperatures and heat index measures spanning the years...

  19. CDC WONDER: Detailed Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Detailed Mortality - Underlying Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2009. Data are...

  20. A pós-graduação e a pesquisa no Serviço Social latino-americano: uma primeira aproximação Postgraduation and research in the Latin American Social Services: a preliminary approach

    OpenAIRE

    Carlos Montaño

    2011-01-01

    O artigo visa apresentar a sistematização dos dados sobre pós-graduação e pesquisa nos países hispano-americanos, a partir de documentos apresentados em dois eventos latino-americanos de pós-graduação. O objetivo é estimular a articulação, os convênios e o intercâmbio entre as unidades de ensino brasileiras e as dos demais países do subcontinente latino-americano.This article presents the systematization of data on postgraduation and research in Spanish American countries Such systematization...

  1. Cdc42 is critical for cartilage development during endochondral ossification.

    Science.gov (United States)

    Suzuki, Wataru; Yamada, Atsushi; Aizawa, Ryo; Suzuki, Dai; Kassai, Hidetoshi; Harada, Takeshi; Nakayama, Mutsuko; Nagahama, Ryo; Maki, Koutaro; Takeda, Shu; Yamamoto, Matsuo; Aiba, Atsu; Baba, Kazuyoshi; Kamijo, Ryutaro

    2015-01-01

    Cdc42 is a widely expressed protein that belongs to the family of Rho GTPases and controls a broad variety of signal transduction pathways in a variety of cell types. To investigate the physiological functions of Cdc42 during cartilage development, we generated chondrocyte-specific inactivated Cdc42 mutant mice (Cdc42(fl/fl); Col2-Cre). The gross morphology of mutant neonates showed shorter limbs and body as compared with the control mice (Cdc42(fl/fl)). Skeletal preparations stained with alcian blue and alizarin red also revealed that the body and the long bone length of the mutants were shorter than those of the control mice. Furthermore, severe defects were found in growth plate chondrocytes in the femur sections of mutant mice, characterized by a reduced proliferating zone height, wider hypertrophic zone, and loss of columnar organization in proliferating chondrocytes. The expression levels of chondrocyte marker genes, such as Col2, Col10, and Mmp13, in mutant mice were decreased as compared with the control mice. Mineralization of trabecular bones in the femur sections was also decreased in the mutants as compared with control mice, whereas osteoid volume was increased. Together these results suggested that chondrocyte proliferation and differentiation in growth plates in the present mutant mice were not normally organized, which contributed to abnormal bone formation. We concluded that Cdc42 is essential for cartilage development during endochondral bone formation. PMID:25343271

  2. Role of AtCDC48 & the AtCDC48 Regulatory Protein Family, PUX, in Plant Cell Morphogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Bednarek, Sebastian, Y.

    2009-11-08

    The long-term objective of this work is to understand the molecular events and mechanisms involved in secretory membrane trafficking and organelle biogenesis, which are crucial for normal plant growth and development. Our studies have suggested a vital role for the cytosolic chaperone Cdc48p/p97 during cytokinesis and cell expansion which are highly dependent upon secretory membrane trafficking. Localization studies have shown that the plant Cdc48p/p97, AtCDC48, and the Arabidopsis ortholog of the ER- and Golgi-associated SNARE, syntaxin 5, (referred to as SYP31) are targeted to the division plane during cytokinesis. In addition, AtCDC48 and SYP31 were shown to interact in vitro and in vivo. To characterize further the function of AtCDC48 and SYP31 we have utilized affinity chromatography and MALDI-MS to identify several plant-specific proteins that interact with SYP31 and/or modulate the activity of AtCDC48 including two UBX (i.e. ubiquitin-like) domain containing proteins, PUX1 and PUX2 (Proteins containing UBX domain). These proteins define a plant protein family consisting of 15 uncharacterized members that we postulate interact with AtCDC48. Biochemical studies have demonstrated that PUX2 is a novel membrane adapter for AtCDC48 that mediates AtCDC48/SYP31 interaction and is likely to control AtCDC48-dependent membrane fusion. In contrast, PUX1 negatively regulates AtCDC48 by inhibiting its ATPase activity and by promoting the disassembly of the active hexamer. These findings provide the first evidence that the assembly and disassembly of the CDC48/p97complex is actually a dynamic process. This new unexpected level of regulation for CDC48/p97 was demonstrated to be critical in vivo as pux1 loss-of-function mutants grow faster than wild-type plants. These studies suggest a role for AtCDC48 in plant cell cycle progression including cytokinesis and/or cell expansion. The proposed studies are designed to: 1) characterize further the localization and function of AtCDC

  3. Políticas e Sistemas de Saúde Ibero-americanos - uma análise comparada: Entrevista de Eleonor Minho Conill.

    OpenAIRE

    Tamires Marinho dos SANTOS; Rackynelly Alves Sarmento SOARES; Ana Valéria Machado MENDONÇA

    2016-01-01

    Entrevista - Eleonor Minho Conill é graduada em Medicina pela Universidade Federal do Rio Grande do Sul, Mestre em Saúde Comunitária pela Université de Montréal e Doutora em Desenvolvimento Econômico e Social pela Université Paris I (Pantheon -Sorbonne). Atuou como docente e pesquisadora nas Universidades Federais do Rio Grande do Sul, Rio de Janeiro, Santa Catarina e na Escola Nacional de Saúde Pública da Fundação Oswaldo Cruz. Trabalha na área de políticas, planejamento, organização, avalia...

  4. Sistema detector d'incendis WSN

    OpenAIRE

    Domínguez López, Emiliano

    2012-01-01

    Sistema detector d'incendis aprofitant una xarxa de sensors sense fils (WSN) mitjançant un sistema encastat. Sistema detector de incendios aprovechando una red de sensores inalámbricos (WSN) mediante un sistema empotrado.

  5. Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea

    Institute of Scientific and Technical Information of China (English)

    ZHENG Wu; CHEN Ji-sheng; ZHENG Shi-qin; LU Guo-dong; WANG Zong-hua

    2006-01-01

    MgCdc42 (Cdc42 in Magnaporthe grisea), with high homology to ScCdc42 (Cdc42 in Saccharomyces cerevisiae), has been demonstrated to involve in the morphogenesis and infection process. To further understand the signaling network,the putative MgCdc42-interacting proteins were analyzed. ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semiquantitative RT-PCR. All retrieved analogs of ScCdc42-interacting proteins from the M.grisea database have conserved domains as those in S. cerevisiae. Expression of their encoding genes increased in MgCdc42CA mutant and decreased in MgCdc42KO mutant. However, MgBem1, Chm1, and MgGic1 in MgCdc42DN mutant had the same expression level as that in the wild type, although MgBem4, MgBoi2, MgCdc24, MgGic2, MgRga1,and Mst20 had decreased expression level, as expected. Overall, it is concluded that there may exist a similar Cdc42 signal pathway in M. grisea as in S. cerevisiae and MgCdc42 plays a key role in the pathway.

  6. As redes de cubanos, norte-americanos e colombianos no tráfico de drogas em miami durante os anos 60

    OpenAIRE

    Sáenz Rovner, Eduardo

    2010-01-01

    Utilizando abundante informação inédita tomada de vários arquivos norte-americanos assim como da imprensa jornalística, especialmente a do sul da Flórida, este artigo estuda o desenvolvimento das redes de traficantes de drogas cubanos, norte-americanos e colombianos em Miami durante os anos 60. O artigo cobre também a entrada massiva de cubanos no sul da Flórida depois da Revolução Cubana, seu desempenho econômico e o clima político na Miami da época. Analisa-se o papel de traficantes cubanos...

  7. Auzolagun: Crowdfunding sistema baten garapena

    OpenAIRE

    Mendiola Larringan, Ander

    2013-01-01

    Memoria dokumentuan Auzolagun izeneko proiektuaren nondik-norakoak azaltzen dira. Sistema Informatikoko Ingeniaritza Teknikoko ikasketa amaierako proiektu hau, Talaios kooperatibarekin elkarlanean sortu da, helburu nagusitzat euskal komunitatearentzako crowdfunding sistema bat sortzea delarik.

  8. Subjetividad y sistemas funcionales

    OpenAIRE

    Lechner, Norbert

    2012-01-01

    En la reseña de mi libro «Las sombras del mañana» (Ed. Lom, 2002), Juan Jiménez plantea un problema que merece discusión: «¿La subjetividad es algo importante, relevante, crucial para las personas o las sociedades?» Jiménez afirma que la subjetividad puede ser importante para las personas, pero -como la sociedad no estaría compuesta por personas, sino por sistemas funcionales-, no tendría importancia para el funcionamiento de la sociedad. Apoyándose en Luhmann, supone que los sistemas obedece...

  9. Desenvolupament sistema ERP

    OpenAIRE

    Duran Rosich, Antoni

    2016-01-01

    Introducció al desenvolupament d'un ERP. El projecte està centrat en el programa més extens de Planificació de Recursos Empresarials o ERP (de l'anglès Enterprise Resource Planning) de Microsoft. Aquests integren (o pretenen integrar) totes les dades i processos d'una organització en un sistema unificat. Aquest sistema utilitza múltiples components de programari i maquinari per aconseguir la integració. Un component clau de la majoria d' ERP és l'ús d'una base de dades única per emmagatzem...

  10. Pruebas de sistemas software

    OpenAIRE

    Fernández Sáez, Aurelio

    2015-01-01

    En el marco de la ingeniería de sistemas, la conferencia versa sobre algunos de los aspectos centrales de la disciplina: la ingeniería de requisitos, la verificación y la validación de sistemas. El eje central es la labor del ingeniero de pruebas y, haciendo uso de un ejemplo, se presentan tanto las ideas clave de esta actividad como la experiencia del conferenciante a lo largo de su trayectoria profesional. Un repaso a las lecciones aprendidas cierra la conferencia

  11. "A wild and wondrous ride": CDC field epidemiologists in the east Pakistan smallpox and cholera epidemics of 1958 "Uma louca e maravilhosa jornada": epidemiologistas de campo do CDC nas epidemias de varíola e cólera do Paquistão Oriental em 1958

    Directory of Open Access Journals (Sweden)

    Paul Greenough

    2011-02-01

    desenvolvimento. Langmuir descreveu o episódio como uma "jornada louca e maravilhosa", porém pareceu mais um pesadelo: o departamento de saúde do Paquistão Oriental entrou em colapso; um movimento popular assumiu a vacinação e desperdiçou suprimentos de vacinas; jornalistas hostis questionaram as motivações ocultas dos americanos; e iniciou-se uma rivalidade profissional entre americanos e o epidemiologista britânico Aidan Cockburn. No final da epidemia, em julho de 1958, tinham sido vacinados trinta milhões de bengalis contra varíola, mas outros vinte mil sucumbiram. Esse episódio foi a primeira intervenção sistemática do CDC no exterior.

  12. Insights into Cdc13 Dependent Telomere Length Regulation

    Energy Technology Data Exchange (ETDEWEB)

    M Mason; E Skordalakes

    2011-12-31

    Cdc13 is a single stranded telomere binding protein that specifically localizes to the telomere ends of budding yeasts and is essential for cell viability. It caps the ends of chromosomes thus preventing chromosome end-to-end fusions and exonucleolytic degradation, events that could lead to genomic instability and senescence, the hallmark of aging. Cdc13 is also involved in telomere length regulation by recruiting or preventing access of telomerase to the telomeric overhang. Recruitment of telomerase to the telomeres for G-strand extension is required for continuous cell division, while preventing its access to the telomeres through capping the chromosome ends prevents mitotic events that could lead to cell immortality, the hall mark of carcinogenesis. Cdc13 and its putative homologues human CTC1 and POT1 are therefore key to many biological processes directly associated with life extension and cancer prevention and can be viewed as an ideal target for cancer and age related therapies.

  13. Cdc42 regulates cofilin during the establishment of neuronal polarity

    DEFF Research Database (Denmark)

    Garvalov, Boyan K; Flynn, Kevin C; Neukirchen, Dorothee;

    2007-01-01

    The establishment of polarity is an essential process in early neuronal development. Although a number of molecules controlling neuronal polarity have been identified, genetic evidence about their physiological roles in this process is mostly lacking. We analyzed the consequences of loss of Cdc42......, a central regulator of polarity in multiple systems, on the polarization of mammalian neurons. Genetic ablation of Cdc42 in the brain led to multiple abnormalities, including striking defects in the formation of axonal tracts. Neurons from the Cdc42 null animals sprouted neurites but had a strongly...... suppressed ability to form axons both in vivo and in culture. This was accompanied by disrupted cytoskeletal organization, enlargement of the growth cones, and inhibition of filopodial dynamics. Axon formation in the knock-out neurons was rescued by manipulation of the actin cytoskeleton, indicating that the...

  14. Uma investigação sobre os co-movimentos na volatilidade dos par bonds latino-americanos

    Directory of Open Access Journals (Sweden)

    Igor A.C. de Morais

    2001-04-01

    Full Text Available Este artigo procura aplicar o método de quase-máxima verossimilhança para estimar a volatilidade estocástica multivariada não-estacionária dos preços de compra dos par bonds de quatro países latino-americanos - México, Brasil, Argentina e Venezuela - no período de 9-8-1994 a 15-9-1999. O objetivo é analisar possíveis movimentos comuns nestas variâncias. Os testes feitos revelam que a volatilidade nos modelos univariados não apresentam inclinação, mas possuem alta persistência. A formulação multivariada relaciona bem os dados, obtendo estimativas consistentes e revelando a existência de um comportamento ao longo do tempo similar entre as volatilidades das quatro séries.This paper uses a quasi-maximum likelihood procedure to estimate the non-stationary stochastic volatility for the par bonds of four Latin American countries: Brazil, Argentina, Mexico and Venezuela. The aim is to investigate the possible presence of co-movements in volatility across countries. The estimation period goes from August 1994 to September 1999, including, therefore, the Asian and Russian crises. The estimated volatility for the univariate model does not show any slope and is highly persistent. The multivariate model gives a good fit to the data and shows that there is common movement.

  15. Las antiguas colecciones arqueológicas de Yucatán en el Museo Americano de Historia Natural

    Directory of Open Access Journals (Sweden)

    Adam T. Sellen

    2009-01-01

    Full Text Available Las investigaciones que hemos desarrollado acerca de la historia de las colecciones yucatecas que forman parte del Museo Americano de Historia Natural de Nueva York nos permiten trazar un perfil general de los intereses intelectuales y de algunas de las primeras exploraciones emprendidas en la región. La información asociada a las piezas arqueológicas que actualmente se resguardan en dicho museo nos remite a los trabajos precursores llevados a cabo por reconocidos viajeros como Charnay, Stephens, Le Plongeon o Maler, y también de otros menos conocidos, como los coleccionistas locales. En conjunto, estos protoarqueólogos enfocaron sus esfuerzos a construir el conocimiento de la antigua civilización maya a través de sus evidencias materiales.Our research on the history of Yucatec collections that are now in the American Museum of Natural History in New York allows us to outline a general profile of the intellectual interests and some of the first explorations in the region. The information associated with the archaeological objects that are today held in this museum speak of the early archaeological work carried out by well known explorers such as Charnay, Stephens, Le Plongeon or Maler, and also by lesser-known players such as the local collectors. Together, these proto-archaeologists focused their efforts on constructing knowledge of the ancient Maya civilization through material evidence.

  16. Informe Signos Vitales de los CDC Obesidad infantil - (Childhood Obesity)

    Centers for Disease Control (CDC) Podcasts

    2013-08-06

    Este podcast se basa en el informe Signos Vitales de los CDC de agosto del 2013. La tasa de obesidad entre los niños en edad prescolar de bajos ingresos ha disminuido, pero todavía uno de cada seis niños hispanos es obeso. Este programa habla brevemente sobre lo que se puede hacer.  Created: 8/6/2013 by Centers for Disease Control and Prevention (CDC).   Date Released: 8/6/2013.

  17. El sistema constructivo

    Directory of Open Access Journals (Sweden)

    Leser S., Heinz

    2003-06-01

    Full Text Available Se describe el sistema constructivo de entramado en madera, “plataforma” (platform frame utilizado preferentemente en las construcciones de varios pisos en el campamento minero de Sewell, Chile./The following is a description of the platform frame used in several stories buildings in the mining settlement Sewell, in Chile.

  18. El sistema constructivo

    OpenAIRE

    Leser S., Heinz; Gómez Lerou, Luis; Salomone R., Vanessa

    2003-01-01

    Se describe el sistema constructivo de entramado en madera, “plataforma” (platform frame) utilizado preferentemente en las construcciones de varios pisos en el campamento minero de Sewell, Chile./The following is a description of the platform frame used in several stories buildings in the mining settlement Sewell, in Chile.

  19. Ambiente Java para sistemas embedded

    OpenAIRE

    Silva, Valter Filipe Miranda Castelão da

    2002-01-01

    Os sistemas integrados, ou genericamente os sistemas com reduzida capacidade de processamento e pouca memória, são actualmente sistemas economicamente acessíveis e muito divulgados. Os seus sistemas operativos estão, no entanto, ainda bastante dependentes do hardware o que faz que cada aplicação seja especialmente desenvolvida para cada tipo de sistema. Neste documento discute-se a aplicabilidade de uma máquina virtual Java em equipamentos com estas restrições. É proposta a ...

  20. Continuous cell injury promotes hepatic tumorigenesis in cdc42-deficient mouse liver

    DEFF Research Database (Denmark)

    van Hengel, Jolanda; D'Hooge, Petra; Hooghe, Bart;

    2008-01-01

    BACKGROUND & AIMS: The Rho small guanosine triphosphatase Cdc42 is critical for diverse cellular functions, including regulation of actin organization, cell polarity, intracellular membrane trafficking, transcription, cell-cycle progression, and cell transformation. This implies that Cdc42 might be...

  1. Cdc42 overexpression induces hyperbranching in the developing mammary gland by enhancing cell migration

    OpenAIRE

    Bray, Kristi; Gillette, Melissa; Young, Jeanette; Loughran, Elizabeth; Hwang, Melissa; Sears, James Cooper; Vargo-Gogola, Tracy

    2013-01-01

    Introduction The Rho GTPase Cdc42 is overexpressed and hyperactivated in breast tumors compared to normal breast tissue. Cdc42 regulates key processes that are critical for mammary gland morphogenesis and become disrupted during the development, progression, and metastasis of breast cancer. However, the contribution of Cdc42 to normal and neoplastic mammary gland development in vivo remains poorly understood. We were therefore interested in investigating the effects of Cdc42 overexpression on...

  2. CDC 24/7: Saving Lives, Protecting People

    Centers for Disease Control (CDC) Podcasts

    2012-06-04

    24/7, CDC provides health information, responds to public health emergencies and natural disasters, and monitors disease.  Created: 6/4/2012 by Office of the Associate Director of Communciation (OADC).   Date Released: 6/4/2012.

  3. CDC Vital Signs: High Blood Pressure and Cholesterol

    Science.gov (United States)

    ... 1.36 MB] Read the MMWR Science Clips High Blood Pressure and Cholesterol Out of Control Recommend on Facebook ... by County http://apps.nccd.cdc.gov/GISCVH2/ High Blood Pressure and High Cholesterol Among US Adults SOURCES: National ...

  4. CDC Reports Link Between Zika Virus and Microcephaly in Brazil

    Science.gov (United States)

    ... news/fullstory_157213.html CDC Reports Link Between Zika Virus and Microcephaly in Brazil And two U.S. women ... Feb. 11, 2016 (HealthDay News) -- Traces of the Zika virus have been identified in the tissue of two ...

  5. Cdc20 control of cell fate during prolonged mitotic arrest

    DEFF Research Database (Denmark)

    Nilsson, Jakob

    2011-01-01

    The fate of cells arrested in mitosis by antimitotic compounds is complex but is influenced by competition between pathways promoting cell death and pathways promoting mitotic exit. As components of both of these pathways are regulated by Cdc20-dependent degradation, I hypothesize that variations...

  6. 13 CFR 120.830 - Reports a CDC must submit.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Reports a CDC must submit. 120.830 Section 120.830 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development... in organizational status; (e) Changes in any condition that affects its eligibility to continue...

  7. Sex Partner with No Zika Symptoms Transmits Virus: CDC

    Science.gov (United States)

    ... news/fullstory_160643.html Sex Partner With No Zika Symptoms Transmits Virus: CDC New report also highlights ties between Zika ... HealthDay News) -- U.S. health officials report that the Zika virus can be spread sexually even when a partner ...

  8. Dangerous Creatures - A Visit to the CDC Insectary

    Centers for Disease Control (CDC) Podcasts

    2012-11-07

    Tour CDC’s insectary with Sofi, a young host, and learn from CDC researchers about mosquitoes and insecticide resistance.  Created: 11/7/2012 by Center for Global Health (CGH).   Date Released: 12/20/2012.

  9. Vaccines for Teens (A Minute of Health with CDC)

    Centers for Disease Control (CDC) Podcasts

    2016-08-25

    CDC recommends children aged 11 to 12 get three vaccines to protect from meningitis; cancers caused by human papillomavirus infections; and tetanus, diphtheria, and pertussis. This podcast discusses vaccination of adolescents.  Created: 8/25/2016 by MMWR.   Date Released: 8/25/2016.

  10. 77 FR 23733 - CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment

    Science.gov (United States)

    2012-04-20

    ... Care Data Systems; (3) External Peer Review of CDC Youth HIV/STI Prevention and Sexual Health..., CDC, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, 1600 Clifton Road, NE... CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment In accordance with section...

  11. 13 CFR 120.810 - Applications for certification as a CDC.

    Science.gov (United States)

    2010-01-01

    ... LOANS Development Company Loan Program (504) Certification Procedures to Become A Cdc § 120.810 Applications for certification as a CDC. (a) An applicant for certification as a CDC must apply to the SBA... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Applications for certification...

  12. Expression of CDC5L is associated with tumor progression in gliomas.

    Science.gov (United States)

    Chen, Wenjuan; Zhang, Li; Wang, Yan; Sun, Jie; Wang, Donglin; Fan, Shaochen; Ban, Na; Zhu, Junya; Ji, Bin; Wang, Yuchan

    2016-03-01

    Cell division cycle 5-like (CDC5L) protein is a cell cycle regulator of the G2/M transition and has been reported to participate in the catalytic step of pre-messenger RNA (mRNA) splicing and DNA damage repair. Recently, it was also found to act as a candidate oncogene in osteosarcoma and cervical tumors. However, the role of CDC5L expression in tumor biology was still unclear. Here, we analyzed the expression and clinical significance of CDC5L in gliomas. The expression of CDC5L in fresh glioma tissues and paraffin-embedded slices was evaluated by western blot and immunohistochemistry, respectively. We found that CDC5L was highly expressed in glioma tissues. The expression of CDC5L was significantly associated with glioma pathology grade and Ki-67 expression. Univariate and multivariate analyses showed that high CDC5L expression was an independent prognostic factor for glioma patients' survival. To determine whether CDC5L could regulate the proliferation of glioma cells, we transfected glioma cells with interfering RNA target CDC5L, then investigated cell proliferation with cell counting kit (CCK)-8, flow cytometry assays and colony formation analyses. Our results indicated that knockdown of CDC5L would inhibit proliferation of glioma cells. Besides, reduced expression of CDC5L could induce the apoptosis of glioma cells. These findings suggested that CDC5L might play an important role in glioma and thus be a promising therapeutic target of glioma. PMID:26490980

  13. LA MIGRACIÓN EN EL SISTEMA INTERAMERICANO DE DERECHOS HUMANOS

    Directory of Open Access Journals (Sweden)

    Verónica Torres-Marenco

    2011-01-01

    Full Text Available El Sistema Interamericano de Derechos Humanos, SIDH, establece unos derechos básicos reconocidos a los migrantes, sin que esto implique un derecho a la migración per se. Estos derechos se encuentran en el marco del principio de no discriminación y la obligación general de respeto de los derechos humanos reconocidos en los instrumentos internacionales. Sin embargo, no ha sido aplicado por los gobiernos nacionales. El desarrollo de sus políticas migratorias se ha dado en violación del derecho a la libertad, la nacionalidad, las garantías judiciales, el debido proceso, etc. Esta situación no parece mejorar en el futuro inmediato, razón por la cual el Sistema Interamericano de Derechos Humanos, SIDH, debe fortalecer su influencia en los estados miembros de la Organización de Estados Americanos, OEA, con el objetivo de discutir no solo el problema del reconocimiento de unos derechos básicos a los inmigrantes indocumentados, sino la pobreza frente al modelo de desarrollo neoliberal en América Latina que constituye una de las causas más frecuentes de la migración.

  14. Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo

    International Nuclear Information System (INIS)

    Gliomas are the most common and aggressive primary brain tumors for which unfortunately no effective treatment modalities exist despite advances in molecular biology as the knowledge base to unravel the extremely complex molecular mechanisms of tumorigenesis is limited. In this study an attempt has been made to understand the molecular pathological basis of tumorigenesis which led to an identification of an oncogene, CDC2, and an epigenetic strategy has been evaluated to control the tumorigensis by downregulating this oncogene. Tissue microarrays were utilized to investigate the expression of genes in a large number of tumor samples and to identify overexpressed genes which could be potentially causing tumorigenesis. Retroviral vectors expressing short hairpin RNAs (shRNAs) targeted against CDC2 were designed and transducted into human glioma cell line ex vivo in order to downregulate the expression of CDC2. Real-Time PCR was used to determine the level of CDC2 mRNA. Western Blotting was used to determine the level of expression of CDC2 protein as measure to quantify down regulation of CDC2 expression along with use of flow cytometry to investigate effect of shRNAs on cell cycles and detection of apoptosis. Following ex vivo study, viral particles containing small interfering RNA for CDC2 were subsequently injected into xenogeneic graft tumor of nude mice and the weight of human glioma xenografts, survival and resulting phenotypic changes of target gene were investigated. Human glioma tissue microarrays indicated the positive expression rates of CDC2/CyclinB1 with a positive correlation with pathologic grades (r = 0.982, r = 0.959, respectively). Retroviral vectors expressing short hairpin RNAs (shRNAs) against CDC2 caused efficient deletion of CDC2, cellular G2/M arrest concluding in apoptosis and inhibition of proliferation in human glioma cells U251 and SHG-44 cell lines ex vivo. And the viral particles containing small interfering RNA for CDC2 were subsequently

  15. Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo

    Directory of Open Access Journals (Sweden)

    Wang Ai-Dong

    2008-01-01

    Full Text Available Abstract Background Gliomas are the most common and aggressive primary brain tumors for which unfortunately no effective treatment modalities exist despite advances in molecular biology as the knowledge base to unravel the extremely complex molecular mechanisms of tumorigenesis is limited. In this study an attempt has been made to understand the molecular pathological basis of tumorigenesis which led to an identification of an oncogene, CDC2, and an epigenetic strategy has been evaluated to control the tumorigensis by downregulating this oncogene. Methods Tissue microarrays were utilized to investigate the expression of genes in a large number of tumor samples and to identify overexpressed genes which could be potentially causing tumorigenesis. Retroviral vectors expressing short hairpin RNAs (shRNAs targeted against CDC2 were designed and transducted into human glioma cell line ex vivo in order to downregulate the expression of CDC2. Real-Time PCR was used to determine the level of CDC2 mRNA. Western Blotting was used to determine the level of expression of CDC2 protein as measure to quantify down regulation of CDC2 expression along with use of flow cytometry to investigate effect of shRNAs on cell cycles and detection of apoptosis. Following ex vivo study, viral particles containing small interfering RNA for CDC2 were subsequently injected into xenogeneic graft tumor of nude mice and the weight of human glioma xenografts, survival and resulting phenotypic changes of target gene were investigated. Results Human glioma tissue microarrays indicated the positive expression rates of CDC2/CyclinB1 with a positive correlation with pathologic grades (r = 0.982, r = 0.959, respectively. Retroviral vectors expressing short hairpin RNAs (shRNAs against CDC2 caused efficient deletion of CDC2, cellular G2/M arrest concluding in apoptosis and inhibition of proliferation in human glioma cells U251 and SHG-44 cell lines ex vivo. And the viral particles

  16. Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1.

    Science.gov (United States)

    Rodriguez-Rodriguez, Jose-Antonio; Moyano, Yolanda; Játiva, Soraya; Queralt, Ethel

    2016-05-31

    To complete mitosis, Saccharomyces cerevisiae needs to activate the mitotic phosphatase Cdc14. Two pathways contribute to Cdc14 regulation: FEAR (Cdc14 early anaphase release) and MEN (mitotic exit network). Cdc5 polo-like kinase was found to be an important mitotic exit component. However, its specific role in mitotic exit regulation and its involvement in Cdc14 release remain unclear. Here, we provide insight into the mechanism by which Cdc5 contributes to the timely release of Cdc14. Our genetic and biochemical data indicate that Cdc5 acts in parallel with MEN during anaphase. This MEN-independent Cdc5 function requires active separase and activation by Cdk1-dependent phosphorylation. Cdk1 first phosphorylates Cdc5 to activate it in early anaphase, and then, in late anaphase, further phosphorylation of Cdc5 by Cdk1 is needed to promote its MEN-related functions. PMID:27210759

  17. sistema Web con JSP

    Directory of Open Access Journals (Sweden)

    César Viloria Núñez

    2014-01-01

    Full Text Available Este artículo presenta el desarrollo de un sistema de información que permite la adquisición y la administración de información relacionada con los signos vitales como la presión arterial, la frecuencia cardiaca y respiratoria, y la saturación de oxígeno en la sangre de un paciente. La implementación del sistema se basa en una solución Web, permitiendo así que médicos especialistas puedan monitorear a sus pacientes desde cualquier punto conectado a la red en tiempo real y, al mismo tiempo, dar indicaciones críticas al personal médico que se encuentra en el lugar con el paciente.

  18. Grandes sistemas fluviais tropicais: uma visão geral

    Directory of Open Access Journals (Sweden)

    Rajiv Sinha

    2010-04-01

    Full Text Available Muitos dos grandes rios e dos leques aluviais existentes estão situados em áreas tropicais e constituem os maiores sistemas de estoque e transferência de sedimentos e nutrientes para o oceano. O presente trabalho visa a apresentação e análise geral dos grandes sistemas fluviais e leques aluviais (área da bacia entre 104 e 6 x 106 km de regiões tropicais com precipitação acima de 700 mm anuais. Os grandes sistemas fluviais tropicais foram aqui classificados de acordo com o arcabouço geológico-geomorfológio de suas bacias em: a Cinturões orogenênicos de montanha, b Platô/plataforma basáltico-sedimentar, c Áreas cratônicas, d Planícies em áreas de bacias sedimentares, e e Áreas mistas. De modo geral, os rios analisados apresentam um pico de descarga bastante definido e variável durante a estação chuvosa e um período de baixa vazão na estação seca. Os dados dos rios sul-americanos e asiáticos de áreas continentais e orogênicas agruparam-se quando lançados num diagrama do suprimento de sedimentos versus descarga média anual, e apresentaram valores mais elevados que aqueles asiáticos insulares. Já rios de áreas de plataforma ou cratônicas, tanto em área de savana como nos trópicos úmidos, caracterizaram-se por terem baixo transporte de sedimento. Especialmente nas grandes bacias, os rios apresentam toda a gama de formas de canal, inviabilizando a classificação tradicional de canal meandrante, reto e entrelaçado, sendo nesse caso mais sugestiva a aplicação da terminologia de sistema uni ou multi-canal ou de complexo anastomosado.É também discutido, brevemente, o atual estado do conhecimento dos sistemas fluviais tropicais e seu potencial na aplicação na interpretação de antigas seqüências e na modelagem de fácies sedimentar. Os principais impactos ambientais a que os grandes sistemas fluviais tropicais estão submetidos incluem as mudanças no uso agrosilpastoril das terras, mineração, barragens

  19. Ciberreligiones: Aproximación al discurso religioso católico y afro-americano en Internet

    Directory of Open Access Journals (Sweden)

    Edgar A. Galavís Añez

    2003-01-01

    Full Text Available El presente artículo, basado en una investigación de carácter exploratorio cualitativo, refiere su corpus de análisis a dos ámbitos del hecho religioso: lo católico-cristiano y lo afro-americano,en relación a su despliegue icónico significante en la red multimediática Internet. El análisis estudiará la imagen como una función semiótica que se manifiesta en forma de discurso dentro de un contexto cultural telecomunicacional. La muestra se refiere a dos portales religiosos, seleccionados según criterios de densidad y calidad informativa: 1. Portal católico cristiano: EWTN/Global Catholic Network. 2.Portal Afroamericano: AxéAfonjá/Candomblé. La presencia de lo religioso en Internet conlleva al estudio de los elementos constitutivos y significantes de sus mensajes en relación al formato ciberespacial. Lo ciberreligioso sería entonces la unión de un nuevo espacio comunicacional con el discurso religioso. El análisis del corpus se apoyará en las retóricas audiovisuales de R. Barthes, U. Eco, en las indagaciones de G.Durandy M. Elia de sobre los arquetipos y los símbolos religiosos así como en la visión antropológica de Pollak Eltz sobre el Candomblé. La lectura se centrará en la estructura discursiva significante de los mitos y símbolos, sus aspectos iconográficos, hipertextuales y etnoescenológicos, en la dinámica diagramática de sus contenidos y en la conformación de sus redes de enlace con ellos mismos y con otras áreas de información paralela y complementaria.

  20. Draft Genome Assemblies of Enterobacter aerogenes CDC 6003-71, Enterobacter cloacae CDC 442-68, and Pantoea agglomerans UA 0804-01.

    Science.gov (United States)

    Minogue, T D; Daligault, H E; Davenport, K W; Bishop-Lilly, K A; Bruce, D C; Chain, P S; Coyne, S R; Chertkov, O; Freitas, T; Frey, K G; Jaissle, J; Koroleva, G I; Ladner, J T; Palacios, G F; Redden, C L; Xu, Y; Johnson, S L

    2014-01-01

    The Enterobacteriaceae are environmental and enteric microbes. We sequenced the genomes of two Enterobacter reference strains, E. aerogenes CDC 6003-71 and E. cloacae CDC 442-68, as well as one near neighbor used as an exclusionary reference for diagnostics, Pantoea agglomerans CDC UA0804-01. The genome sizes range from 4.72 to 5.55 Mbp and have G+C contents from 54.6 to 55.1%. PMID:25342683

  1. Draft Genome Assemblies of Enterobacter aerogenes CDC 6003-71, Enterobacter cloacae CDC 442-68, and Pantoea agglomerans UA 0804-01

    OpenAIRE

    Minogue, T. D.; Daligault, H. E.; Davenport, K. W.; Bishop-Lilly, K. A.; Bruce, D. C.; Chain, P. S.; Coyne, S. R.; Chertkov, O.; Freitas, T.; Frey, K. G.; Jaissle, J.; Koroleva, G. I.; Ladner, J. T.; Palacios, G. F.; Redden, C. L.

    2014-01-01

    The Enterobacteriaceae are environmental and enteric microbes. We sequenced the genomes of two Enterobacter reference strains, E. aerogenes CDC 6003-71 and E. cloacae CDC 442-68, as well as one near neighbor used as an exclusionary reference for diagnostics, Pantoea agglomerans CDC UA0804-01. The genome sizes range from 4.72 to 5.55 Mbp and have G+C contents from 54.6 to 55.1%.

  2. Cdc18 transcription and proteolysis couple S phase to passage through mitosis.

    OpenAIRE

    Baum, B.; Nishitani, H; Yanow, S; Nurse, P

    1998-01-01

    In fission yeast, cdc18p plays a critical role in bringing about the onset of S phase. We show that cdc18p expression is subject to a complex sequence of cell cycle controls which ensure that cdc18p levels rise dramatically as cells exit mitosis, before the appearance of CDK activity in G1. We find that transcription of cdc18, together with the transcription of other cdc10p/res1p targets, is first initiated as cells enter mitosis and continues even in cells arrested in mitosis with highly con...

  3. Sistemas Silvipastoris Silvipastoral Systems

    Directory of Open Access Journals (Sweden)

    Fernando Salgado Bernardino

    2010-02-01

    Full Text Available

    Apesar das pesquisas com sistemas silvipastoris terem sido iniciadas no final da década de 1970, as informações geradas até hoje não são em grande número. As vantagens advindas de um sistema silvipastoril são inúmeras e devidamente reconhecidas. Pelo fato de ser uma técnica de uso da terra capaz de recuperar ecossistemas alterados pelo mau manejo, estes sistemas, pela integração de atividades agrícolas, pecuárias e silviculturais, passam a representar uma tecnologia que confere maior sustentabilidade que os sistemas tradicionais, nos quais os monocultivos são predominantes. Nos últimos anos, instituições de ensino, pesquisa e extensão do País têm se voltado para o desenvolvimento de tais sistemas. O governo de Minas Gerais, por meio de sua Secretaria de Estado de Agricultura e de órgãos vinculados, vem promovendo a condução de modelos agrossilvipastoris num processo integrado de ocupação do solo, denominado lavoura-pecuária-silvicultura. Apesar dos grandes avanços no conhecimento de culturas anuais e dos componentes arbóreos, principalmente o eucalipto, e de gramíneas forrageiras tolerantes à diminuição da intensidade luminosa, ainda é necessário se comnhecer melhor o manejo do sub-bosque sob os efeitos do pastejo.

     

    doi: 10.4336/2009.pfb.60.77

    Although researches related to silvopastoral systems have been initiated many years ago in Brazil, there are few informations generated until now. The advantages from a silvopastoral system are inumerous and well recognized. By the fact of being a technique of land using capable of recovering several disturbed ecosystems, the silvopastoral systems by the integration of agricultural, cattle, and silvicultural activities represent a technology that can achieve higher sustainability than the traditional systems as the monocultures. Today, research centers in Brazil dedicate great attention to agrossilvopastoral systems. The government of Minas Gerais

  4. Expression of CDc6 after acute spinal cord injury in adult rats.

    Science.gov (United States)

    Chen, Chen; Lu, Jian; Yu, Qin; Xiao, Jian-Ru; Wei, Hai-Feng; Song, Xin-Jian; Ge, Jian-Bing; Tao, Wei-Dong; Qian, Rong; Yu, Xiao-Wei; Zhao, Jian

    2016-04-01

    The cell division cycle 6 (CDc6) protein has been primarily investigated as a component of the pre-replicative complex for the initiation of DNA replication. Some studies have shown that CDc6 played a critical role in the development of human carcinoma. However, the expression and roles of CDc6 in the central nervous system remain unknown. We have performed an acute spinal cord injury (SCI) model in adult rats and investigated the dynamic changes of CDc6 expression in spinal cord. Western blot have found that CDc6 protein levels first significantly increase, reach a peak at day 3, and then gradually return to normal level at day 14 after SCI. Double immunofluorescence staining showed that CDc6 immunoreactivity was found in neurons, astrocytes, and microglia. Additionally, colocalization of CDc6/active caspase-3 has been detected in neurons and colocalization of CDc6/proliferating cell nuclear antigen has been detected in astrocytes and microglial. In vitro, CDc6 depletion by short interfering RNA inhibits astrocyte proliferation and reduces cyclin A and cyclin D1 protein levels. CDc6 knockdown also decreases neuronal apoptosis. We speculate that CDc6 might play crucial roles in CNS pathophysiology after SCI. PMID:26899166

  5. Sistemas de software distribuidos : Experiencias

    OpenAIRE

    Pesado, Patricia Mabel; Ramón, Hugo Dionisio; Thomas, Pablo Javier; Boracchia, Marcos; Bertone, Rodolfo Alfredo; Ibañez, Eduardo; Estrebou, César Armando; Marrero, Luciano; Delía, Lisandro; Galdámez, Nicolás; Cáseres, Germán

    2011-01-01

    Se presenta una línea de investigación y desarrollo, que tiene por objeto estudiar temas relacionados con aspectos de Ingeniería de Software orientados al desarrollo e implementación de proyectos concretos de Sistemas Distribuidos, en particular Sistemas Distribuidos con restricciones de Tiempo Real, Sistemas Web y Sistemas Sensibles al Contexto, considerando las extensiones necesarias en las metodologías y herramientas clásicas. En este sentido se trabaja en aspectos de la Ingeniería de Soft...

  6. A América Latina, uma justaposição de dois sistemas de proteção de direitos humanos?

    Directory of Open Access Journals (Sweden)

    Tenile Mascolo

    2015-08-01

    Full Text Available O MERCOSUL tem evoluído no estabelecimento de um sistema de promoção e proteção dos Direitos Humanos, principalmente graças ao desenvolvimento de uma estrutura institucional e normativa voltada à promoção e à proteção desses direitos. Tendo em vista que a maioria de seus Estados Partes são todos membros da Organização dos Estados Americanos, esses países se veem consequentemente sob os auspícios dos dois sistemas de proteção de Direitos Humanos, o que pode ser motivo para embates ou colaborações. Pergunta-se então de quem é a competência na apreciação de questões de violações referentes aos Direitos Humanos no seio do MERCOSUL: de um controle interno realizado pelos órgãos que compõem o sistema de resolução de conflitos do MERCOSUL ou de um controle externalizado pelos órgãos do sistema interamericano de proteção de Direitos Humanos?

  7. Diversidad genética de la población colombiana de ganado Cebú Brahman Americano Bos Indicus (Bovidae)

    OpenAIRE

    Novoa Bravo Miguel Adriano; Usaquén Martínez William

    2006-01-01

    La raza Cebú Brahman Americano se encuentra en Colombia alrededor de 100 años. Todo ese tiempo, esta raza ha estado bajo un proceso continuo de selección artificial dirigida, reproducción endogámica, efectos de deriva genética causados por eventos fundadores, migraciones de ejemplares entre las fincas del país y animales importados desde otros países. Estos hechos hacen a esta raza interesante y particular desde el punto de vista de la genética de poblaciones. El objetivo de este trabajo es e...

  8. A Amazônia no imaginário norte-americano em tempo de guerra Amazonia in the U.S. wartime imaginary

    OpenAIRE

    Seth Garfield

    2009-01-01

    Este ensaio delineia a emergência da Amazônia no imaginário norte-americano no período da Segunda Guerra Mundial. Embora a importância geopolítica de diversificação dos mercados fornecedores de borracha e o desenvolvimento da borracha sintética tenham sido discutidos nos Estados Unidos antes do ataque a Pearl Harbor, a subsequente interdição japonesa aos mercados tradicionais do sudeste asiático levou burocratas e cientistas a se apressarem na busca de alternativas. Como em outras regiões da ...

  9. Representações políticas do território latino-americano na Revista Seleções

    OpenAIRE

    Mary A. Junqueira

    2001-01-01

    Este artigo tem por objetivo analisar as representações sobre a América Latina construídas pela revista Seleções do Reader's Digest, entre 1942 e 1970, e mostra como essas representações estiveram informadas pela idéia de wilderness, profundamente enraizada no imaginário norte-americano.This article intends to analyse the representations about Latin America produced by Seleções do Reader's Digest magazine, from 1942 to 1970. It demonstrates that these representations were influenced by wilder...

  10. Representações políticas do território latino-americano na Revista Seleções

    Directory of Open Access Journals (Sweden)

    Mary A. Junqueira

    2001-01-01

    Full Text Available Este artigo tem por objetivo analisar as representações sobre a América Latina construídas pela revista Seleções do Reader's Digest, entre 1942 e 1970, e mostra como essas representações estiveram informadas pela idéia de wilderness, profundamente enraizada no imaginário norte-americano.This article intends to analyse the representations about Latin America produced by Seleções do Reader's Digest magazine, from 1942 to 1970. It demonstrates that these representations were influenced by wilderness imaginary, which is deeply rooted in North American culture.

  11. A atuação da Organização dos Estados Americanos (OEA) nas crises políticas contemporâneas

    OpenAIRE

    Pedro Ernesto Fagundes

    2010-01-01

    Este artigo analisa a atuação da Organização dos Estados Americanos (OEA) no período pós Guerra Fria, especificamente, nas crises políticas do Haiti (1991), Venezuela (2001) e Honduras (2009). O trabalho apresenta um panorama da atuação dessa organização multilateral a partir da década de 1950 até as mais recentes disputas ocorridas na América Latina.

  12. A atuação da Organização dos Estados Americanos (OEA nas crises políticas contemporâneas

    Directory of Open Access Journals (Sweden)

    Pedro Ernesto Fagundes

    2010-04-01

    Full Text Available Este artigo analisa a atuação da Organização dos Estados Americanos (OEA no período pós Guerra Fria, especificamente, nas crises políticas do Haiti (1991, Venezuela (2001 e Honduras (2009. O trabalho apresenta um panorama da atuação dessa organização multilateral a partir da década de 1950 até as mais recentes disputas ocorridas na América Latina.

  13. Two Cdc2 Kinase Genes with Distinct Functions in Vegetative and Infectious Hyphae in Fusarium graminearum.

    Directory of Open Access Journals (Sweden)

    Huiquan Liu

    2015-06-01

    Full Text Available Eukaryotic cell cycle involves a number of protein kinases important for the onset and progression through mitosis, most of which are well characterized in the budding and fission yeasts and conserved in other fungi. However, unlike the model yeast and filamentous fungi that have a single Cdc2 essential for cell cycle progression, the wheat scab fungus Fusarium graminearum contains two CDC2 orthologs. The cdc2A and cdc2B mutants had no obvious defects in growth rate and conidiation but deletion of both of them is lethal, indicating that these two CDC2 orthologs have redundant functions during vegetative growth and asexual reproduction. However, whereas the cdc2B mutant was normal, the cdc2A mutant was significantly reduced in virulence and rarely produced ascospores. Although deletion of CDC2A had no obvious effect on the formation of penetration branches or hyphopodia, the cdc2A mutant was limited in the differentiation and growth of infectious growth in wheat tissues. Therefore, CDC2A plays stage-specific roles in cell cycle regulation during infectious growth and sexual reproduction. Both CDC2A and CDC2B are constitutively expressed but only CDC2A was up-regulated during plant infection and ascosporogenesis. Localization of Cdc2A- GFP to the nucleus but not Cdc2B-GFP was observed in vegetative hyphae, ascospores, and infectious hyphae. Complementation assays with chimeric fusion constructs showed that both the N- and C-terminal regions of Cdc2A are important for its functions in pathogenesis and ascosporogenesis but only the N-terminal region is important for its subcellular localization. Among the Sordariomycetes, only three Fusarium species closely related to F. graminearum have two CDC2 genes. Furthermore, F. graminearum uniquely has two Aurora kinase genes and one additional putative cyclin gene, and its orthologs of CAK1 and other four essential mitotic kinases in the budding yeast are dispensable for viability. Overall, our data

  14. Controlabilidad de Sistemas Bilineales en el Plano

    OpenAIRE

    Laura Guarachi, Leonardo Ramiro

    2012-01-01

    El objeto principal de este trabajo son los sistemas de ecuaciones diferenciales que dependen de un parámetro u, control del sistema, tales sistemas se llaman sistemas de ecuaciones diferenciales controlables, los cuales, en general, describen procesos dinámicos de fenómenos físicos. Estos procesos se describen mediante sistemas de ecuaciones diferenciales ordinarios (EDO), ecuaciones en diferencias o a través de ecuaciones derivadas parciales. Una clase de sistemas de mucha im...

  15. Sistema de Reconocimiento de Caracteres Orientado a Sistemas Android

    OpenAIRE

    Segura Morales, Oriol

    2014-01-01

    Este proyecto trata sobre un sistema de reconocimiento de caracteres que se implementa para sistemas Android en forma de aplicación para móvil y Tablet. La aplicación está orientada para que los niños aprendan a escribir de una forma más amena. El sistema utiliza el patrón de diseño Modelo-Vista-Controlador para separar la lógica del sistema de la interfaz visual. En la interfaz, el sistema pide que se escriba un carácter utilizando la pantalla táctil y una vez el usuario lo ha escrito el sis...

  16. CDC Vital Signs-Communication Can Save Lives

    Centers for Disease Control (CDC) Podcasts

    2015-08-04

    This podcast is based on the August 2015 CDC Vital Signs report. Antibiotic-resistant germs cause at least 23,000 deaths each year. Learn how public health authorities and health care facilities can work together to save lives.  Created: 8/4/2015 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 8/4/2015.

  17. CDC Vital Signs-Hospital Actions Affect Breastfeeding

    Centers for Disease Control (CDC) Podcasts

    2015-10-06

    This podcast is based on the October 2015 CDC Vital Signs report. Hospitals can implement the Ten Steps to Successful Breastfeeding to be designated as "Baby-Friendly" and support more moms in a decision to breastfeed.  Created: 10/6/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/6/2015.

  18. CDC Vital Signs-Safer Food Saves Lives

    Centers for Disease Control (CDC) Podcasts

    2015-11-03

    This podcast is based on the November 2015 CDC Vital Signs report. Contaminated food sent to several states can cause multistate outbreaks of foodborne illness and make a lot of people seriously ill. Learn what can be done to prevent and stop outbreaks.  Created: 11/3/2015 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 11/3/2015.

  19. CDC Vital Signs-Protect Patients from Antibiotic Resistance

    Centers for Disease Control (CDC) Podcasts

    2016-03-03

    This podcast is based on the March 2016 CDC Vital Signs report. Patients can get serious healthcare-associated infections, or HAIs, while receiving medical treatment in a healthcare facility. Learn how to prevent healthcare-associated infections.  Created: 3/3/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 3/3/2016.

  20. Health communication takes on new dimensions at CDC.

    OpenAIRE

    Roper, W L

    1993-01-01

    Actions by the Centers for Disease Control and Prevention (CDC) to integrate health communication into overall prevention programs as a means of influencing individual behavior to reduce risks to health are described. These actions include a set of 5-year goals for the Agency; a proposal to establish an Office of Health Communication to provide leadership and support for accomplishing the goals; and establishment of a working group to create the proposed Office of Health Communication and to ...

  1. Cyclin-dependent kinase CDK1/CDC28 and checkpoints

    International Nuclear Information System (INIS)

    The genetic instability induced by defects in the cell cycle progression contributes to different human diseases, particularly neoplastic transformation. The control mechanisms of correct cell cycle progression are the most studied in the yeast Saccharomyces cerevisiae, in which checkpoint was first discovered. Many components of these processes have been identified by now. Here, the role of the central kinase of cell cycle CDK1/CDC28 is considered in checkpoint in different phases.

  2. Sistemas constructivos industrializados

    Directory of Open Access Journals (Sweden)

    del Águila, Alfonso

    1996-12-01

    Full Text Available The present article summarizes the main points discussed in three Innovator Buildings Systems sessions, which are about different subjects of Industrialized Building. The first part is about the topic of concrete panels, applied to building flats basically. It is given a historical summary and the general concepts, ending with "INDAGSA BUILDING SYSTEM", which is running several promotions with in Madrid. In the second part it is developed the subject of building with boxes, beginning with general points which are extended to the concept of box, its classification, its historical aspects and its applications. It is continues with a description of the "SET HOME SYSTEM" of boxes of concrete, which is dedicated to industrialization of social housing services and equipments. This part ends with the French system "TRANSLOKO" of big light boxes. Lately, it has made a building for laboratories in Madrid. The last part is dedicated to a new subject in Interior Building, which is the Dry Floor, being detailedly described the "PLADUR" point of view about this.

    El presente artículo resume lo tratado en tres jornadas de Sistemas Constructivos Innovadores, relativos a diversos temas de Construcción Industrializada. En la primera parte se trata el tema de los paneles de hormigón aplicados a la edificación, principalmente a viviendas. Se da una síntesis histórica y esquemática de conceptos generales, para terminar con una descripción del Sistema Constructivo INDAGSA que están realizando numerosas promociones en Madrid. La segunda parte, desarrolla el tema de la construcción con módulos tridimensionales, comenzando por unas consideraciones generales que abarcan el concepto de módulo tridimensional, su clasificación, aspectos históricos y campos de aplicación. Se sigue con una descripción del Sistema SET HOME, de módulos tridimensionales de hormigón, dedicado a la industrialización de servicios e instalaciones en vivienda social

  3. Certificiranje sistema vodenja kakovosti

    OpenAIRE

    HVALA, JERNEJ

    2016-01-01

    Hiter in spremenljiv tehnološki razvoj je vse pogosteje narekoval vse večje zahteve po kontroliranju in evidentiranju kakovosti. Posledično so se s tem razvijali in oblikovali tudi novi in oprijemljivejši standardi za obvladovanje sistemov kakovosti. Z uvedbo sistema vodenja kakovosti lahko določena organizacija doseže številne prednosti in izboljša svoje poslovanje znotraj družbe. Kot prednosti lahko izpostavimo predvsem: preglednejšo organiziranost poslovanja, dokumentiranost postopkov...

  4. Sistema psiconeuroendocrino y envejecimiento

    OpenAIRE

    Antón Alvarez, X.; Franco, Andrés; Cacabelos, Ramón

    1990-01-01

    Este trabajo expone los efectos del envejecimiento sobre los distintos ejes del sistema neuroendocrino. El envejecimiento provoca cambios dramáticos en la mayor parte de ellos. Algunas de estas alteraciones van asociadas a los cambios conductuales y al rendimiento mental propios del envejecimiento normal y patológico. El mejor conocimiento del envejecimiento neuroendocrino permitirá en su día emplear fármacos y técnicas derivadas de la neurobiología y la genética molecular en ...

  5. Sistema Brain Computer Interface

    OpenAIRE

    Martín Barraza, Juan Ignacio

    2015-01-01

    En este trabajo de final de grado se realizará una aplicación de un sistema Brain Computer Interface en el cual, a partir del dipositivo Mind Wave de la compañía Neurosky, se pretenderá controlar el prototipo de una mano humana. Esta será controlada a partir de las ondas cerebrales medidas por el sensor que el dispositivo dispone. A continuación, la información captada por nuestro medidor de señales de electroencefalográficas será enviada por radiofrecuencia a un stick USB que viene incorpora...

  6. Sistemas de autoconsumo fotovoltaico

    OpenAIRE

    Pereira, Carlos Alberto da Conceição

    2015-01-01

    O presente relatório é desenvolvido no âmbito da unidade curricular de estágio do Mestrado em Engenharia Eletrotécnica com especialização em Controlo e Eletrónica Industrial e apresenta o meu trabalho na empresa na qual sou responsável pela parte de projeto, dimensionamento e supervisão de obra de sistemas fotovoltaicos para produção de energia. Os projetos apresentados neste relatório são da minha autoria. A empresa, Critical Kinetics apresenta-se no mercado como Consultores de energia...

  7. Qualidade da notificação de anomalias congênitas pelo Sistema de Informações sobre Nascidos Vivos (SINASC): estudo comparativo nos anos 2004 e 2007 Quality of birth defect reporting in the Brazilian Information System on Live Births (SINASC): a comparative study of 2004 and 2007

    OpenAIRE

    Daniela Varela Luquetti; Rosalina Jorge Koifman

    2010-01-01

    Este estudo comparou a validade dos diagnósticos de anomalias congênitas do Sistema de Informações sobre Nascidos Vivos (SINASC), em oito hospitais distribuídos em sete municípios do Brasil, totalizando 27.945 nascidos vivos em 2004 e 25.905 em 2007. Além disso, descreveu ações específicas realizadas para o aprimoramento da qualidade dos dados desse campo. Para a análise da validade, foi utilizado o Estudo Colaborativo Latino-Americano de Malformações Congênitas (ECLAMC) como padrão-ouro. Em ...

  8. Plants of the American continent with antimalarial activity Plantas do continente Americano com atividade antimalárica

    Directory of Open Access Journals (Sweden)

    Ingrid R. Mariath

    2009-03-01

    Full Text Available Malaria is a human parasitic disease caused by protozoa species of the Plasmodium genus. This disease has affected populations of the tropical and subtropical regions. About 500 million new cases occur annually on the world and therefore it is considered an emerging disease of important public health problem. In this context, the natural products as vegetables species have their bioactive molecules as targets for pharmacological, toxicological and phytochemical studies towards the development of more effective medicines for the treatment of many diseases. So this work intends to aid the researchers in the study of natural products to the treatment of malaria. In this review, 476 plants of the American continent were related for the antimalarial activity and of these vegetables species 198 were active and 278 inactive for some type of Plasmodium when they were evaluated through of in vitro or in vivo bioassays models.Malária é uma doença parasitária humana causada por protozoários do gênero Plasmodium. Esta doença tem acometido populações que habitam regiões tropicais e subtropicais. Anualmente, cerca de 500 milhões de casos ocorrem no mundo, o que permite ser considerada uma doença emergente de importância para a saúde pública. Neste contexto, os produtos naturais, a exemplo das espécies vegetais, têm suas moléculas bioativas como alvo para estudos farmacológicos, toxicológicos e fitoquímicos destinados à síntese de medicamentos mais eficazes para o tratamento de inúmeras doenças. Portanto, este trabalho fornece subsídio às pesquisas com produtos naturais para o tratamento da malária. Nesta revisão, 476 espécies de plantas do continente Americano foram relatadas para a atividade antimalárica, sendo destas 198 ativas e 278 inativas para algum tipo de Plasmodium, quando avaliados através de modelos in vitro e in vivo.

  9. El oso negro americano en el noroeste de México: recuperación de registros de ocurrencia

    Directory of Open Access Journals (Sweden)

    Christian Alejandro Delfín-Alfonso

    2011-12-01

    Full Text Available El conocimiento sobre la distribución de la mayoría de las especies es incompleto y fragmentado, debido a la falta de registros en colecciones científicas u observaciones fidedignas, como es el caso del oso negro americano (Ursus americanus particularmente al NO de México. El objetivo fue recuperar datos históricos y actuales de ocurrencia de la especie en la ecorregión de la Sierra Madre Occidental y las Islas del Cielo. Se recopilaron registros de diversas colecciones y museos (nacionales y extranjeras, estudios históricos y actuales (artículos científicos, informes, tesis, reportes sin publicar e informantes clave. Se obtuvieron 88 registros (10 ♂, 10 ♀, 68 sexo indeterminado en un periodo de 120 años (1890-2010. De colecciones científicas se obtuvieron 54 registros, 29 fueron obtenidos de la literatura y cinco de informantes confiables. La mayoría de los registros se ubicaron espacialmente dentro de cinco Áreas Naturales Protegidas y seis Regiones Terrestres Prioritarias. Los Estados de Chihuahua y Sonora contaron con más registros, colectados en el período de 2001-2010; para los periodos de 1921-1930 y 1941-1950 no se obtuvieron registros. El bajo número de registros obtenidos es reflejo de la falta de estudios a lo largo de la distribución de la especie en México, y la carencia de reportes formales sobre avistamientos, capturas o muertes de los animales a nivel local. El presente trabajo contribuye sustancialmente a identificar y examinar los vacios geográficos de información respecto a la especie, funcionando como línea base para la toma de decisiones, principalmente para aplicar técnicas y métodos que ayuden a seleccionar sitios prioritarios para su conservación. Es imperante reconocer la necesidad de implementar estrategias de manejo y conservación de la especie, a fin de poder mantenerla a largo plazo en nuestro país.

  10. 13 CFR 120.837 - SBA decision on application for a new CDC or for an existing CDC to expand Area of Operations.

    Science.gov (United States)

    2010-01-01

    ... benchmarks, including pre-approval and annual review by SBA of any management or staff contracts, and the... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA decision on application for a new CDC or for an existing CDC to expand Area of Operations. 120.837 Section 120.837 Business...

  11. Índices del Anuario de Estudios Americanos (tomos XLII-LI, 2, años 1985-1994

    Directory of Open Access Journals (Sweden)

    Sarabia Viejo, M.ª Justina

    1999-06-01

    Full Text Available Los índices que se incluyen a continuación tratan de aportar la información más útil a los investigadores americanistas. Para ello se ha establecido una división en índices alfabético, geográfico y por materias, además de situar al principio la relación de los artículos publicados en cada número del Anuario de Estudios Americanos durante esa década. Desde 1993, a partir del Tomo L (dividido en números 1 y 2 se ha unificado con la otra revista hasta entonces también publicada en la Escuela de Estudios Hispanoamericanos, del CSIC, con los nombres de Historiografía y Bibliografía Americanistas y Suplemento de Anuario de Estudios Americanos. Sección Historiografía y Bibliografía.

  12. El siglo perdido del Derecho Administrativo americano: más allá de un desfase histórico

    Directory of Open Access Journals (Sweden)

    Manuela Canal Silva

    2013-12-01

    Full Text Available Creando la Constitución Administrativa. Los Cien Años Perdidos del Derecho Administrativo americano, elegido por la American Bar Association –ABA– como el mejor trabajo de Derecho Administrativo publicado en el 2012, es considerado una de las más polémicas y exhaustivas exposiciones alrededor de la evolución del gobierno estadinense2. Acorde con las palabras de Robert L. Rabin (s.f., la obra es una contribución sustancial al entendimiento del gobierno administrativo en los Estados Unidos en sus orígenes y actualidad. Este artículo presenta las ideas más importantes del trabajo de Mashaw analizando la evolución de la Administración desde los primeros días de la República americana. Con este fin, explora la configuración del gobierno americano antes y después de la guerra civil. En conclusión, el artículo desarrolla la exposición de Mashaw y explora las implicaciones de su obra en las disciplinas histórica y jurídica americanas.

  13. EUROPA Y SU PERCEPCIÓN DEL NUEVO MUNDO A TRAVÉS DE LAS ESPECIES COMESTIBLES Y LOS ESPACIOS AMERICANOS EN EL SIGLO XVI

    Directory of Open Access Journals (Sweden)

    OLAYA SANFUENTES ECHEVERRÍA

    2006-12-01

    Full Text Available El encuentro europeo con las nuevas especies y espacios americanos en el siglo XVI, complejiza ese universo de percepciones de superioridad occidental frente a mundos que se consideraban más exóticos y menos civilizados. Las descripciones europeas de la naturaleza americana, las primeras imágenes de sus productos alimenticios y especies autóctonas pintadas con los colores del paraíso, el exotismo y la belleza, la fertilidad y la abundancia, ayudarán a conformar una imagen positiva del continente americano. A pesar del original desprecio o indiferencia frente a ciertos alimentos indígenas, con el tiempo estas percepciones cambiarán al convertirse muchos de ellos en salvavidas de las hambrunas mundiales e ingredientes fundamentales de las gastronomías nacionalesThe European encounter with the new American space and it species during the sixteenth century, allows us to look at the European percepction of superiority towards exotic worlds in a more complex way. European description of American nature, the first visual images of the local species described with the colors of paradise, exotism and beauty, fertility and abundance, help in constructing a positive image of America. Despite the original disdain or indifference towards the indigenous food, these percepctions will change over time when many of these products become a solution to world wide hunger and important ingredients for national gastronomies

  14. Los indios americanos en las escenas líricas europeas: de los hermanos Purcell (1695 a Carl Heinrich Graun (1755

    Directory of Open Access Journals (Sweden)

    Núñez Ronchi, Ana

    2014-08-01

    Full Text Available In contrast to a supposedly critically-oriented Golden Age comedia, the first American- themed operas (the Purcells, Vivaldi, Rameau, Graun have been interpreted as uncritical expressions of the so-called Black Legend. Nevertheless, this judgment ought to be tempered: on the one hand, the Spanish comedia is not as censorious as has been asserted; on the other hand, although these operas do praise American Indians, they do not systematically belittle the Spaniards. Finally, the notion itself of “Black Legend” should be questioned.Se ha querido interpretar las primeras óperas de tema americano (Purcell, Vivaldi, Rameau y Graun como una expresión dramático-musical de la llamada Leyenda Negra, contrapuesta a una producción teatral áurea española que, inversamente, sí habría dejado espacio a la dimensión histórico-crítica. Ello nos parece injusto e inexacto: en primer lugar, la comedia áurea no escenifica la dimensión crítica que se ha pretendido atribuirle; en segundo lugar, el teatro lírico europeo, si bien exalta al indio americano, no devalúa sistemáticamente a los conquistadores españoles; finalmente, la noción misma de «Leyenda Negra» debe ser problematizada.

  15. Um exemplo de inversão do domínio ideológico norte-americano no Brasil

    Directory of Open Access Journals (Sweden)

    Carla Damião

    2014-07-01

    Full Text Available Propomos discutir a relação de domínio ideológico-cultural norte-americano sobre o Brasil, tendo em vista exemplos documentados. Trata-se da chamada “política de boa vizinhança” [Good Neighbor Policy], com início em 1933 até o final da Segunda Guerra, notadamente durante o governo Getúlio Vargas no Brasil e o governo Franklin Roosevelt nos Estados Unidos. A necessidade de propaganda política passava pela “sedução” cultural da potência estadunidense sobre seus vizinhos latino-americanos. Neste sentido, o momento político de tentativa de “adoção” ideológica do Brasil pelos Estados Unidos foi marcado pela “encomenda” de produtos culturais como a personagem do malandro Zé Carioca de Disney. O exemplo mais ambíguo que marca essa ambição de domínio ideológico é o filme encomendado a Orson Welles e jamais finalizado, intitulado: It’s All True [É tudo verdade]. O filme não concluído por seu famoso diretor, filmado em 1942, só se tornou conhecido em 1993 quando outros diretores americanos realizaram um documentário remontando partes do filme. O material presente nesse documentário foi também recuperado pela pesquisa realizada pelo cineasta brasileiro Rogério Sganzerla, da qual surgiu uma tetralogia composta pelos filmes: Nem tudo é verdade (1986, A liguagem de orson Welles (1990, Tudo é Brasil (1997 e O signo do caos (2003. O projeto de domínio cultural encomendado a Orson Welles terminou em fracasso, visto que este desviou sua atenção da glamourosa cidade do Rio de Janeiro e do Carnaval, para filmar a saga de jangadeiros que lutavam por direitos trabalhistas. A conotação política do filme determina seu fracasso no domínio ideológico de propaganda política do governo americano. Sganzerla elegeu o episódio para pensar e criticar o domínio ideológico norte-americano sempre presente na cultura brasileira, de uma maneira bastante ambígua. A ambiguidade permeia a atitude dos dois cineastas: o

  16. Oxidative stress-induced DNA damage of mouse zygotes triggers G2/M checkpoint and phosphorylates Cdc25 and Cdc2.

    Science.gov (United States)

    Zhang, Yuting; Qian, Diting; Li, Zhiling; Huang, Yue; Wu, Que; Ru, Gaizhen; Chen, Man; Wang, Bin

    2016-07-01

    In vitro fertilized (IVF) embryos show both cell cycle and developmental arrest. We previously showed oxidative damage activates the ATM → Chk1 → Cdc25B/Cdc25C cascade to mediate G2/M cell cycle arrest for repair of hydrogen peroxide (H2O2)-induced oxidative damage in sperm. However, the mechanisms underlying the developmental delay of zygotes are unknown. To develop a model of oxidative-damaged zygotes, we treated mouse zygotes with different concentrations of H2O2 (0, 0.01, 0.02, 0.03, 0.04, 0.05 mM), and evaluated in vitro zygote development, BrdU incorporation to detect the duration of S phase. We also examined reactive oxygen species level and used immunofluorescence to detect activation of γH2AX, Cdc2, and Cdc25. Oxidatively damaged zygotes showed a delay in G2/M phase and produced a higher level of ROS. At the same time, γH2AX was detected in oxidatively damaged zygotes as well as phospho-Cdc25B (Ser323), phospho-Cdc25C (Ser216), and phospho-Cdc2 (Tyr15). Our study indicates that oxidative stress-induced DNA damage of mouse zygotes triggers the cell cycle checkpoint, which results in G2/M cell cycle arrest, and that phospho-Cdc25B (Ser323), phospho-Cdc25C (Ser216), and phospho-Cdc2 (Tyr15) participate in activating the G2/M checkpoint. PMID:27117522

  17. Identification of a novel protein interaction between Elmo1 and Cdc27.

    Science.gov (United States)

    Lee, Juyeon; Moon, Byeongjin; Lee, Dae-Hee; Lee, Gwangrog; Park, Daeho

    2016-03-18

    Elmo has no intrinsic catalytic activity but coordinate multiple cellular processes via their interactions with other proteins. Studies thus have been focused on identifying Elmo binding partners, but the number of characterized Elmo-interacting proteins remains limited. Here, we report Cdc27 as a novel Elmo1-interacting protein. In yeast and mammalian cells, Cdc27 specifically interacted with the C-terminal region of Elmo1 essential for Dock1 association and function. The interaction of Elmo1 with Dock1 abrogated binding between Elmo1 and Cdc27, but the Dock1-Elmo1 interaction was unaffected by Cdc27. Similarly, cellular phagocytotic functions mediated by the Elmo1-Dock1-Rac module were unaffected by Cdc27 levels. In summary, a novel binding partner, Cdc27, was identified for Elmo1 and they appear to be independent of Elmo-Dock1-Rac-mediated processes. PMID:26882976

  18. Implementation experiences of NASTRAN on CDC CYBER 74 SCOPE 3.4 operating system

    Science.gov (United States)

    Go, J. C.; Hill, R. G.

    1973-01-01

    The implementation of the NASTRAN system on the CDC CYBER 74 SCOPE 3.4 Operating System is described. The flexibility of the NASTRAN system made it possible to accomplish the change with no major problems. Various sizes of benchmark and test problems, ranging from two hours to less than one minute CP time were run on the CDC CYBER SCOPE 3.3, Univac EXEC-8, and CDC CYBER SCOPE 3.4. The NASTRAN installation deck is provided.

  19. Polo kinase Cdc5 is a central regulator of meiosis I

    OpenAIRE

    Attner, Michelle A.; Miller, Matthew P.; Ee, Ly-Sha; Elkin, Sheryl K; Amon, Angelika

    2013-01-01

    During meiosis, two consecutive rounds of chromosome segregation yield four haploid gametes from one diploid cell. The Polo kinase Cdc5 is required for meiotic progression, but how Cdc5 coordinates multiple cell-cycle events during meiosis I is not understood. Here we show that CDC5-dependent phosphorylation of Rec8, a subunit of the cohesin complex that links sister chromatids, is required for efficient cohesin removal from chromosome arms, which is a prerequisite for meiosis I chromosome se...

  20. A national, geographic database of CDC-funded HIV prevention services: development challenges and potential applications

    OpenAIRE

    Fogarty Kieran J; Gilliam Aisha; Gibbs Deborah A; Hanchette Carol L; Bruhn Mark

    2005-01-01

    Abstract Background From 2000–2002, the Centers for Disease Control and Prevention (CDC) funded a study that was designed to improve the information available to program planners about the geographic distribution of CDC-funded HIV prevention services provided by community-based organizations (CBOs). Program managers at CDC recognized the potential of a geographic information system (GIS) to organize and analyze information about HIV prevention services and they made GIS a critical component o...

  1. FMNL2 drives actin-based protrusion and migration downstream of Cdc42

    DEFF Research Database (Denmark)

    Block, Jennifer; Breitsprecher, Dennis; Kühn, Sonja;

    2012-01-01

    -guanosine triphosphatase Cdc42. Abolition of myristoylation or Cdc42 binding interferes with proper FMNL2 activation, constituting an essential prerequisite for subcellular targeting. In vitro, C-terminal FMNL2 drives elongation rather than nucleation of actin filaments in the presence of profilin. In addition, filament...... establish that the FMNL subfamily member FMNL2 is a novel elongation factor of actin filaments that constitutes the first Cdc42 effector promoting cell migration and actin polymerization at the tips of lamellipodia....

  2. Opposing Roles for Actin in Cdc42p PolarizationD⃞

    OpenAIRE

    Irazoqui, Javier E.; Howell, Audrey S.; Theesfeld, Chandra L.; Lew, Daniel J.

    2005-01-01

    In animal and fungal cells, the monomeric GTPase Cdc42p is a key regulator of cell polarity that itself exhibits a polarized distribution in asymmetric cells. Previous work showed that in budding yeast, Cdc42p polarization is unaffected by depolymerization of the actin cytoskeleton (Ayscough et al., J. Cell Biol. 137, 399–416, 1997). Surprisingly, we now report that unlike complete actin depolymerization, partial actin depolymerization leads to the dispersal of Cdc42p from the polarization si...

  3. miR-330 regulates the proliferation of colorectal cancer cells by targeting Cdc42

    International Nuclear Information System (INIS)

    Highlights: ► miR-330 was inversely correlated with Cdc42 in colorectal cancer cells. ► Elevated miR-330 suppressed cell proliferation in vivo and in vitro. ► Elevated miR-330 mimicked the effect of Cdc42 knockdown. ► Restoration of Cdc42 could partially attenuate the effects of miR-330. -- Abstract: MicroRNAs are small non-coding RNA molecules that play important roles in the multistep process of colorectal carcinoma (CRC) development. However, the miRNA–mRNA regulatory network is far from being fully understood. The objective of this study was to investigate the expression and the biological roles of miR-330 in colorectal cancer cells. Cdc42, one of the best characterized members of the Rho GTPase family, was found to be up-regulated in several types of human tumors including CRC and has been implicated in cancer initiation and progression. In the present study, we identified miR-330, as a potential regulator of Cdc42, was found to be inversely correlated with Cdc42 expression in colorectal cancer cell lines. Ectopic expression of miR-330 down-regulated Cdc42 expression at both protein and mRNA level, mimicked the effect of Cdc42 knockdown in inhibiting proliferation, inducing G1 cell cycle arrest and apoptosis of the colorectal cancer cells, whereas restoration of Cdc42 could partially attenuate the effects of miR-330. In addition, elevated expression of miR-330 could suppress the immediate downstream effectors of Cdc42 and inhibit the growth of colorectal cancer cells in vivo. To sum up, our results establish a role of miR-330 in negatively regulating Cdc42 expression and colorectal cancer cell proliferation. They suggest that manipulating the expression level of Cdc42 by miR-330 has the potential to influence colorectal cancer progression

  4. Las virtudes del sistema binominal

    OpenAIRE

    John M Carey

    2006-01-01

    El sistema único de elecciones legislativas chileno, en el que cada distrito elige a dos representantes (M=2), ha sido objeto de controversia y de propuestas de reforma, ya que fue impuesto por el régimen autoritario saliente en 1990. La mayor parte de ellas aboga por la llamada mayor proporcionalidad. Este ensayo sostiene que, a pesar de sus orígenes no democráticos, el sistema M=2 ha funcionado relativamente bien respecto a los sistemas empleados en muchos otros países. En particular, M=2 e...

  5. Sistema de salud de Chile

    OpenAIRE

    Víctor Becerril-Montekio; Juan de Dios Reyes; Annick Manuel

    2011-01-01

    En este trabajo se describe el sistema de salud de Chile, incluyendo su estructura, financiamiento, beneficiarios y recursos físicos, materiales y humanos de los que dispone. Este sistema está compuesto por dos sectores, público y privado. El sector público está formado por todos los organismos que constituyen el Sistema Nacional de Servicios de Salud y cubre aproximadamente a 70% de la población, incluyendo a los pobres del campo y las ciudades, la clase media baja y los jubilados, así como ...

  6. Sistema polític espanyol: sistema electoral

    OpenAIRE

    Fernández Herrero, Myriam

    2012-01-01

    Materials docents programats mitjançant l'ajut del Servei de Política Lingüística de la Universitat de València. Explicacion de las funciones del sistema electoral en abstracto, de los elementos constitutivos y cómo lo definen. Aplicación al sistema electoral estatal, autonómico, local y europeo

  7. Rho GTPase protein Cdc42 is critical for postnatal cartilage development.

    Science.gov (United States)

    Nagahama, Ryo; Yamada, Atsushi; Tanaka, Junichi; Aizawa, Ryo; Suzuki, Dai; Kassai, Hidetoshi; Yamamoto, Matsuo; Mishima, Kenji; Aiba, Atsu; Maki, Koutaro; Kamijo, Ryutaro

    2016-02-19

    Cdc42, a small Rho GTPase family member, has been shown to regulate multiple cellular functions in vitro, including actin cytoskeletal reorganization, cell migration, proliferation, and gene expression. However, its tissue-specific roles in vivo remain largely unknown, especially in postnatal cartilage development, as cartilage-specific Cdc42 inactivated mice die within a few days after birth. In this study, we investigated the physiological functions of Cdc42 during cartilage development after birth using tamoxifen-induced cartilage-specific inactivated Cdc42 conditional knockout (Cdc42 (fl/fl); Col2-CreERT) mice, which were generated by crossing Cdc42 flox mice (Cdc42 (fl/fl)) with tamoxifen-induced type II collagen (Col2) Cre transgenic mice using a Cre/loxP system. The gross morphology of the Cdc42 cKO mice was shorter limbs and body, as well as reduced body weight as compared with the controls. In addition, severe defects were found in growth plate chondrocytes of the long bones, characterized by a shorter proliferating zone (PZ), wider hypertrophic zone (HZ), and loss of columnar organization of proliferating chondrocytes, resulting in delayed endochondral bone formation associated with abnormal bone growth. Our findings demonstrate the importance of Cdc42 for cartilage development during both embryonic and postnatal stages. PMID:26820532

  8. Low cdc27 and high securin expression predict short survival for breast cancer patients.

    Science.gov (United States)

    Talvinen, Kati; Karra, Henna; Pitkänen, Reino; Ahonen, Ilmari; Nykänen, Marjukka; Lintunen, Minnamaija; Söderström, Mirva; Kuopio, Teijo; Kronqvist, Pauliina

    2013-10-01

    Cell cycle regulators cdc27 and securin participate in control of the mitotic checkpoint and survey the mitotic spindle to maintain chromosomal integrity. This is achieved by their functions in metaphase-anaphase transition, DNA damage repair, enhancement of mitotic arrest and apoptosis. We report on the roles of cdc27 and securin in aneuploidy and prognosis of breast cancer. The study comprises 429 breast cancer patients with up to 22 years of follow-up. DNA content was determined by image cytometry, and immunopositivity for cdc27 and securin was based on tissue microarrays. An inverse association between cdc27 and securin expression was observed in both image cytometric and immunohistochemical analyses. Low cdc27 and high securin expression identified patients with significant difference in disease outcome. Cdc27 and securin immunoexpression identified patients at risk of early cancer death within five years from diagnosis. In multivariate analysis, the combination of cdc27 and securin immunohistochemistry was the strongest predictor of cancer death after lymph node status. We demonstrate, for the first time in human breast cancer, the prognostic value of cdc27 and securin immunohistochemistry. Cdc27 and securin appear promising biomarkers for applications in predicting disease progression, prognostication of individual patients and potential in anti-mitotic drug development. PMID:23755904

  9. Cdc20 and securin overexpression predict short-term breast cancer survival

    Science.gov (United States)

    Karra, H; Repo, H; Ahonen, I; Löyttyniemi, E; Pitkänen, R; Lintunen, M; Kuopio, T; Söderström, M; Kronqvist, P

    2014-01-01

    Background: Cdc20 is an essential component of cell division and responsible for anaphase initiation regulated by securin degradation. Cdc20 function is strongly regulated by the spindle assembly checkpoint to ensure the timely separation of sister chromatids and integrity of the genome. We present the first results on Cdc20 in a large clinical breast cancer material. Methods: The study was based on 445 breast cancer patients with up to 20 years of follow-up (mean 10.0 years). DNA content was determined by image cytometry on cell imprints, and Cdc20 and securin immunohistochemistry on tissue microarrays of breast cancer tissue. Results: In our results, high Cdc20 and securin expression was associated with aneuploid DNA content. In prognostic analyses, high Cdc20 immunoexpression alone and in combination with high securin immunoexpression indicated aggressive course of disease and up to 6.8-fold (P<0.001) risk of breast cancer death. Particularly, high Cdc20 and securin immunoexpression identified a patient subgroup with extremely short, on average 2.4 years, breast cancer survival and triple-negative breast cancer (TNBC) subtype. Conclusions: We report for the first time the association of high Cdc20 and securin immunoexpression with extremely poor outcome of breast cancer patients. Our experience indicates that Cdc20 and securin are promising candidates for clinical applications in breast cancer prognostication, especially in the challenging prognostic decisions of TNBC. PMID:24853182

  10. Telomerase Recruitment in Saccharomyces cerevisiae Is Not Dependent on Tel1-Mediated Phosphorylation of Cdc13

    Science.gov (United States)

    Gao, Hua; Toro, Tasha B.; Paschini, Margherita; Braunstein-Ballew, Bari; Cervantes, Rachel B.; Lundblad, Victoria

    2010-01-01

    In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action. A current model proposes that Tel1 binding to telomeres marks them for elongation, as the result of phosphorylation of a proposed S/TQ cluster in the telomerase recruitment domain of Cdc13. However, three observations presented here argue against one key aspect of this model. First, the pattern of Cdc13 phosphatase-sensitive isoforms is not altered by loss of Tel1 function or by mutations introduced into two conserved serines (S249 and S255) in the Cdc13 recruitment domain. Second, an interaction between Cdc13 and Est1, as monitored by a two-hybrid assay, is dependent on S255 but Tel1-independent. Finally, a derivative of Cdc13, cdc13–(S/TQ)11→(S/TA)11, in which every potential consensus phosphorylation site for Tel1 has been eliminated, confers nearly wild-type telomere length. These results are inconsistent with a model in which the Cdc13–Est1 interaction is regulated by Tel1-mediated phosphorylation of the Cdc13 telomerase recruitment domain. We propose an alternative model for the role of Tel1 in telomere homeostasis, which is based on the assumption that Tel1 performs the same molecular task at double-strand breaks (DSBs) and chromosome termini. PMID:20837994

  11. Cdc13 N-Terminal Dimerization DNA Binding and Telomere Length Regulation

    Energy Technology Data Exchange (ETDEWEB)

    M Mitchell; J Smith; M Mason; S Harper; D Speicher; F Johnson; E Skordalakes

    2011-12-31

    The essential yeast protein Cdc13 facilitates chromosome end replication by recruiting telomerase to telomeres, and together with its interacting partners Stn1 and Ten1, it protects chromosome ends from nucleolytic attack, thus contributing to genome integrity. Although Cdc13 has been studied extensively, the precise role of its N-terminal domain (Cdc13N) in telomere length regulation remains unclear. Here we present a structural, biochemical, and functional characterization of Cdc13N. The structure reveals that this domain comprises an oligonucleotide/oligosaccharide binding (OB) fold and is involved in Cdc13 dimerization. Biochemical data show that Cdc13N weakly binds long, single-stranded, telomeric DNA in a fashion that is directly dependent on domain oligomerization. When introduced into full-length Cdc13 in vivo, point mutations that prevented Cdc13N dimerization or DNA binding caused telomere shortening or lengthening, respectively. The multiple DNA binding domains and dimeric nature of Cdc13 offer unique insights into how it coordinates the recruitment and regulation of telomerase access to the telomeres.

  12. Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p

    OpenAIRE

    Gladfelter, Amy S.; Bose, Indrani; Zyla, Trevin R.; Bardes, Elaine S.G.; Lew, Daniel J.

    2002-01-01

    At the beginning of the budding yeast cell cycle, the GTPase Cdc42p promotes the assembly of a ring of septins at the site of future bud emergence. Here, we present an analysis of cdc42 mutants that display specific defects in septin organization, which identifies an important role for GTP hydrolysis by Cdc42p in the assembly of the septin ring. The mutants show defects in basal or stimulated GTP hydrolysis, and the septin misorganization is suppressed by overexpression of a Cdc42p GTPase-act...

  13. Amino acid residues in the CDC25 guanine nucleotide exchange factor critical for interaction with Ras.

    OpenAIRE

    Park, W.; Mosteller, R D; Broek, D.

    1994-01-01

    Previously we found that negatively charged residues at positions 62, 63, and 69 of H-Ras are involved in binding to the CDC25 guanine nucleotide exchange factor (GEF). Using site-directed mutagenesis, we have changed conserved, positively charged residues of CDC25GEF to glutamic acid. We find the nonfunctional CDC25R1374E mutant and the nonfunctional H-RasE63K mutant cooperate in suppression of the loss of CDC25 function in Saccharomyces cerevisiae. Also, peptides corresponding to residues 1...

  14. The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras.

    OpenAIRE

    Jones, S; Vignais, M L; Broach, J R

    1991-01-01

    The product of the CDC25 gene of Saccharomyces cerevisiae, in its capacity as an activator of the RAS/cyclic AMP pathway, is required for initiation of the cell cycle. In this report, we provide an identification of Cdc25p, the product of the CDC25 gene, and evidence that it promotes exchange of guanine nucleotides bound to Ras in vitro. Extracts of strains containing high levels of Cdc25p catalyze both removal of GDP from and the concurrent binding of GTP to Ras. This same activity is also o...

  15. Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13.

    Science.gov (United States)

    Gao, Hua; Toro, Tasha B; Paschini, Margherita; Braunstein-Ballew, Bari; Cervantes, Rachel B; Lundblad, Victoria

    2010-12-01

    In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action. A current model proposes that Tel1 binding to telomeres marks them for elongation, as the result of phosphorylation of a proposed S/TQ cluster in the telomerase recruitment domain of Cdc13. However, three observations presented here argue against one key aspect of this model. First, the pattern of Cdc13 phosphatase-sensitive isoforms is not altered by loss of Tel1 function or by mutations introduced into two conserved serines (S249 and S255) in the Cdc13 recruitment domain. Second, an interaction between Cdc13 and Est1, as monitored by a two-hybrid assay, is dependent on S255 but Tel1-independent. Finally, a derivative of Cdc13, cdc13-(S/TQ)11→(S/TA)11, in which every potential consensus phosphorylation site for Tel1 has been eliminated, confers nearly wild-type telomere length. These results are inconsistent with a model in which the Cdc13-Est1 interaction is regulated by Tel1-mediated phosphorylation of the Cdc13 telomerase recruitment domain. We propose an alternative model for the role of Tel1 in telomere homeostasis, which is based on the assumption that Tel1 performs the same molecular task at double-strand breaks (DSBs) and chromosome termini. PMID:20837994

  16. Diversidad genética de la población colombiana de ganado Cebú Brahman Americano Bos Indicus (Bovidae

    Directory of Open Access Journals (Sweden)

    Novoa Bravo Miguel Adriano

    2006-12-01

    Full Text Available La raza Cebú Brahman Americano se encuentra en Colombia alrededor de 100 años. Todo ese tiempo, esta raza ha estado bajo un proceso continuo de selección artificial dirigida, reproducción endogámica, efectos de deriva genética causados por eventos fundadores, migraciones de ejemplares entre las fincas del país y animales importados desde otros países. Estos hechos hacen a esta raza interesante y particular desde el punto de vista de la genética de poblaciones. El objetivo de este trabajo es estudiar la estructura y diversidad genética de la raza Cebú Brahman americano. Se utilizaron 162 animales registrados en la asociación colombiana de criadores de ganado cebú (ASOCEBU de 20 departamentos de Colombia. La genotipificación de los animales se llevó a cabo con el kit StockMarks® for cattle bovine genotyping de Applied Biosystems®, empleando 10 microsatélites dinucleótidos. Los resultados de los distintos análisis multivariados (Análisis de componentes principales y análisis de correspondencias múltiples, de inferencia bayesiana y distancias genéticas interindividuales, demuestran que no se presenta subestructura en la población, lo cual se explica por una alta tasa de migración de animales entre las diferentes fincas y regiones, que homogeniza las frecuencias en todo el país. Además, esta población posee un alto grado de heterocigocidad y diversidad alélica, comparado con otras razas, lo cual refleja su origen de mezcla multiracial. También se encontraron diferencias genéticas entre sexos, lo cual es causado por un proceso reproductivo diferencial, donde actúan diferentes criterios de selección entre sexos. Finalmente, al realizar un análisis de componentes principales para analizar las relaciones genéticas de Cebú Brahman americano colombiano con las razas cebuinas y taurinas, se determinó que esta raza se diferencia genéticamente de las demás razas cebuinas, debido a un aporte genético de razas taurinas

  17. CDC Vital Signs-Daily Pill Can Prevent HIV

    Centers for Disease Control (CDC) Podcasts

    2015-11-24

    This podcast is based on the November 24, 2015 CDC Vital Signs report. Preexposure prophylaxis, or PrEP, is a daily medicine that can be used to prevent getting HIV. PrEP is for people who don’t have HIV but who are at very high risk for getting it from sex or injection drug use. Unfortunately, many people who can benefit from PrEP aren’t taking it.  Created: 11/24/2015 by National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP).   Date Released: 11/24/2015.

  18. CDC Vital Signs-E-cigarette Ads and Youth

    Centers for Disease Control (CDC) Podcasts

    2016-01-05

    This podcast is based on the January 2016 CDC Vital Signs report. Most electronic cigarettes, or e-cigarettes, contain nicotine, which is highly addictive and may harm brain development. More than 18 million middle and high school students were exposed to e-cigarette ads. Exposure to these ads may be contributing to an increase in e-cigarette use among youth. Learn what can be done to keep our youth safe and healthy.  Created: 1/5/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 1/5/2016.

  19. Uma teoria da preponderância do Poder Executivo: o sistema de comissões no Legislativo brasileiro

    Directory of Open Access Journals (Sweden)

    Pereira Carlos

    2000-01-01

    Full Text Available O objetivo do artigo é analisar o sistema de comissões na Câmara dos Deputados à luz das teorias da escolha racional (distributiva, informacional e partidária desenvolvidas para explicar o Congresso norte-americano. Em que pese a enorme preponderância do Executivo sobre a agenda do Legislativo, este trabalho enfatiza que as comissões continuam funcionando ativamente e desempenham um papel importante para os parlamentares, os partidos e para o próprio Executivo. Inicialmente, tenta-se justificar a existência e funcionamento das comissões no Brasil com base na literatura distributiva. No entanto, como será demonstrado, esta explicação não pareceu ser suficientemente forte para justificar o atual sistema de comissões. Em seguida, através de uma adaptação da teoria informacional de Gilligan e Krehbiel (1997 para as especificidades das instituições do Congresso nacional, é testada uma hipótese usando os dados da legislatura 1995-1998. Os resultados da regressão multivariada (probit na escolha de procedimentos de urgência e não urgência (variável dependente fornecem evidências empíricas de que as comissões na Câmara dos Deputados têm de fato, pelo menos parcialmente, um papel informacional.

  20. Multisite phosphorylation of the guanine nucleotide exchange factor Cdc24 during yeast cell polarization.

    Directory of Open Access Journals (Sweden)

    Stephanie C Wai

    Full Text Available BACKGROUND: Cell polarization is essential for processes such as cell migration and asymmetric cell division. A common regulator of cell polarization in most eukaryotic cells is the conserved Rho GTPase, Cdc42. In budding yeast, Cdc42 is activated by a single guanine nucleotide exchange factor, Cdc24. The mechanistic details of Cdc24 activation at the onset of yeast cell polarization are unclear. Previous studies have suggested an important role for phosphorylation of Cdc24, which may regulate activity or function of the protein, representing a key step in the symmetry breaking process. METHODOLOGY/PRINCIPAL FINDINGS: Here, we directly ask whether multisite phosphorylation of Cdc24 plays a role in its regulation. We identify through mass spectrometry analysis over thirty putative in vivo phosphorylation sites. We first focus on sites matching consensus sequences for cyclin-dependent and p21-activated kinases, two kinase families that have been previously shown to phosphorylate Cdc24. Through site-directed mutagenesis, yeast genetics, and light and fluorescence microscopy, we show that nonphosphorylatable mutations of these consensus sites do not lead to any detectable consequences on growth rate, morphology, kinetics of polarization, or localization of the mutant protein. We do, however, observe a change in the mobility shift of mutant Cdc24 proteins on SDS-PAGE, suggesting that we have indeed perturbed its phosphorylation. Finally, we show that mutation of all identified phosphorylation sites does not cause observable defects in growth rate or morphology. CONCLUSIONS/SIGNIFICANCE: We conclude that lack of phosphorylation on Cdc24 has no overt functional consequences in budding yeast. Yeast cell polarization may be more tightly regulated by inactivation of Cdc42 by GTPase activating proteins or by alternative methods of Cdc24 regulation, such as conformational changes or oligomerization.

  1. CDC Signos Vitales: Enfermedad del legionario (CDC Vital Signs–Legionnaires’ Disease)

    Centers for Disease Control (CDC) Podcasts

    2016-06-07

    Este podcast se basa en la edición de junio del 2016 del informe Signos Vitales de los CDC. Las personas pueden contraer la enfermedad del legionario, un tipo grave de infección pulmonar, al inhalar pequeñas gotitas de agua contaminada con bacterias Legionella. Obtenga más información sobre lo que se puede hacer para ayudar a prevenir brotes de enfermedad del legionario y mantener seguras a las personas.  Created: 6/7/2016 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 6/7/2016.

  2. Modelo de avaliação de uma opção de venda tipo Americano: solução analítica.

    OpenAIRE

    Viegas, Cristina; Pereira, José Azevedo

    2005-01-01

    Este estudo desenvolve uma solução analítica para o valor de uma opção de venda de tipo Americano e para o respectivo preço crítico. Para obter uma solução fechada da equação diferencial proposta por Black e Scholes (1973), utiliza-se um procedimento usualmente conhecido como “método das linhas” - considera-se uma formulação na qual o tempo é discreto, em vez de contínuo. Deste modo, a equação diferencial parcial é transformada numa equação diferencial ordinária não homogénea, a derivad...

  3. Trained to interact: echoes from the Workshop Sul-Americano de Mediação em Museus e Centros de Ciência (Italian original version

    Directory of Open Access Journals (Sweden)

    Luisa Massarani

    2008-12-01

    Full Text Available The initiatives focusing the professional development of explainers are multiplying around the world, building an informal network of researchers, museums managers and directors, explainers, and regional/continental networks, as THE group, the Thematic Human Interface and Explainers group of Ecsite.The Workshop Sul-Americano de Mediação em Museus e Centros de Ciência e Escola de Mediação em Museus e Centros de Ciência, which took place in Rio de Janeiro in September 2008, was a further important step along this path. We believe it is worthwhile to offer to Jcom readers some of the workshop contributions concerning the training of explainers, to which we added an overview of the general problem presented by Lynn Uyen Tran (Lawrence Hall of Science at the University of California, Berkeley.

  4. En portada SISTEMA RESPIRATORIO

    Directory of Open Access Journals (Sweden)

    Andrés J. Flores Alés,

    2005-10-01

    Full Text Available La sección “En portada” de este mes está dedicada a los agentes infecciosos y al diagnóstico de algunas de las enfermedades respiratorias más habituales en los animales de compañía, estacando la relevancia adquirida por el diagnóstico mediante la obtención de de imágenes en este campo. También se hace incapie en la actuación que debe llevar a cabo elínico ante una urgencia respiratoria La obtención de imágenes por radiología o endoscopia son dos de las técnicas diagnósticas que más ayudan a dilucidar el proceso respiratorio que sufre un paciente. Sobre el momento en el que se encuentra el diagnóstico por imagen de las patologías del sistema respiratorio en veterinaria nos habla a continuación Andrés J. Flores.

  5. The human homolog of S. cerevisiae CDC27, CDC27 Hs, is encoded by a highly conserved intronless gene present in multiple copies in the human genome

    Energy Technology Data Exchange (ETDEWEB)

    Devor, E.J.; Dill-Devor, R.M. [Univ. of Iowa College of Medicine, Iowa City (United States)

    1994-09-01

    We have obtained a number of unique sequences via PCR amplification of human genomic DNA using degenerate primers under low stringency (42{degrees}C). One of these, an 853 bp product, has been identified as a partial genomic sequence of the human homolog of the S. cerevisiae CDC27 gene, CDC27Hs (GenBank No. U00001). This gene, reported by Turgendreich et al. is also designated EST00556 from Adams et al. We have undertaken a more detailed examination of our sequence, MCP34N, and have found that: 1. the genomic sequence is nearly identical to CDC27Hs over its entire 853 bp length; 2. an MCP34N-specific PCR assay of several non-human primate species reveals amplification products in chimpanzee and gorilla genomes having greater than 90% sequence identity with CDC27Hs; and 3. an MCP34N-specific PCR assay of the BIOS hybrid cell line panel gives a discordancy pattern suggesting multiple loci. Based upon these data, we present the following initial characterization: 1. the complete MCP34N sequence identity with CDC27Hs indicates that the latter is encoded by an intronless gene; 2. CDC27Hs is highly conserved among higher primates; and 3. CDC27Hs is present in multiple copies in the human genome. These characteristics, taken together with those initially reported for CDC27Hs, suggest that this is an old gene that carries out an important but, as yet, unknown function in the human brain.

  6. The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface

    DEFF Research Database (Denmark)

    Cappello, Silvia; Attardo, Alessio; Wu, Xunwei; Iwasato, Takuji; Itohara, Shigeyoshi; Wilsch-Bräuninger, Michaela; Eilken, Hanna M; Rieger, Michael A; Schroeder, Timm T; Huttner, Wieland B; Brakebusch, Cord; Götz, Magdalena

    2006-01-01

    fundamental difference between these progenitors. Here we show that the conditional deletion of the small Rho-GTPase cdc42 at different stages of neurogenesis in mouse telencephalon results in an immediate increase in basal mitoses. Whereas cdc42-deficient progenitors have normal cell cycle length...

  7. Cdc42 expression in keratinocytes is required for the maintenance of the basement membrane in skin

    DEFF Research Database (Denmark)

    Wu, Xunwei; Quondamatteo, Fabio; Brakebusch, Cord

    2006-01-01

    , structure and number of hemidesomosomes were not significantly changed in the Cdc42 mutant skin compared with the control mice and no blister formation was observed in mutant skin. These data indicate that Cdc42 in keratinocytes is important for maintenance of the basement membrane of skin....

  8. CDC's Response to the 2014-2016 Ebola Epidemic - Guinea, Liberia, and Sierra Leone.

    Science.gov (United States)

    Dahl, Benjamin A; Kinzer, Michael H; Raghunathan, Pratima L; Christie, Athalia; De Cock, Kevin M; Mahoney, Frank; Bennett, Sarah D; Hersey, Sara; Morgan, Oliver W

    2016-01-01

    CDC's response to the 2014-2016 Ebola virus disease (Ebola) epidemic in West Africa was the largest in the agency's history and occurred in a geographic area where CDC had little operational presence. Approximately 1,450 CDC responders were deployed to Guinea, Liberia, and Sierra Leone since the start of the response in July 2014 to the end of the response at the end of March 2016, including 455 persons with repeat deployments. The responses undertaken in each country shared some similarities but also required unique strategies specific to individual country needs. The size and duration of the response challenged CDC in several ways, particularly with regard to staffing. The lessons learned from this epidemic will strengthen CDC's ability to respond to future public health emergencies. These lessons include the importance of ongoing partnerships with ministries of health in resource-limited countries and regions, a cadre of trained CDC staff who are ready to be deployed, and development of ongoing working relationships with U.S. government agencies and other multilateral and nongovernment organizations that deploy for international public health emergencies. CDC's establishment of a Global Rapid Response Team in June 2015 is anticipated to meet some of these challenges. The activities summarized in this report would not have been possible without collaboration with many U.S. and international partners (http://www.cdc.gov/vhf/ebola/outbreaks/2014-west-africa/partners.html). PMID:27388930

  9. 13 CFR 120.820 - CDC non-profit status and good standing.

    Science.gov (United States)

    2010-01-01

    ..., as determined by SBA in its discretion. The CDC's Risk Rating, among other factors, will be... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false CDC non-profit status and good standing. 120.820 Section 120.820 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION...

  10. Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy.

    Directory of Open Access Journals (Sweden)

    Ronan Broderick

    Full Text Available Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our data show that eGFP-Cdc45 mobility is faster in G1/S transition compared to S phase suggesting that Cdc45 is part of larger protein complex formed in S phase. Furthermore, the size of complexes containing Cdc45 was estimated in asynchronous, G1/S and S phase-synchronized cells using gel filtration chromatography; these findings complemented the in vivo FCS data. Analysis of the mobility of eGFP-Cdc45 and the size of complexes containing Cdc45 and eGFP-Cdc45 after UVC-mediated DNA damage revealed no significant changes in diffusion rates and complex sizes using FCS and gel filtration chromatography analyses. This suggests that after UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage.

  11. 77 FR 58847 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2012-09-24

    ... HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) In accordance with section 10(a)(2) of the Federal... enable CDC to fulfill its mission of protecting health through health promotion, prevention,...

  12. Multiple alterations of platelet functions dominated by increased secretion in mice lacking Cdc42 in platelets

    DEFF Research Database (Denmark)

    Pleines, Irina; Eckly, Anita; Elvers, Margitta; Hagedorn, Ina; Eliautou, Sandra; Bender, Markus; Wu, Xunwei; Lanza, Francois; Gachet, Christian; Brakebusch, Cord; Nieswandt, Bernhard

    2010-01-01

    (ADP)/adenosine triphosphate (ATP) content, increased aggregation at low agonist concentrations, and enhanced aggregate formation on collagen under flow. In vivo, lack of Cdc42 resulted in faster occlusion of ferric chloride-injured arterioles. The life span of Cdc42-deficient platelets was markedly...

  13. Cdc42 Controls Vascular Network Assembly Through Protein Kinase C{iota} During Embryonic Vasculogenesis

    DEFF Research Database (Denmark)

    Qi, Yanmei; Liu, Jie; Wu, Xunwei; Brakebusch, Cord; Leitges, Michael; Han, Yaling; Corbett, Siobhan A; Lowry, Stephen F; Graham, Alan M; Li, Shaohua

    2011-01-01

    The goal of this study was to determine the role of Cdc42 in embryonic vasculogenesis and the underlying mechanisms.......The goal of this study was to determine the role of Cdc42 in embryonic vasculogenesis and the underlying mechanisms....

  14. High expression of CDC6 is associated with accelerated cell proliferation and poor prognosis of epithelial ovarian cancer.

    Science.gov (United States)

    Deng, Yan; Jiang, Lifei; Wang, Yingying; Xi, Qinghua; Zhong, Jianxin; Liu, Jian; Yang, Shuyun; Liu, Rong; Wang, Juan; Huang, Menghui; Tang, Chunhui; Su, Min

    2016-04-01

    Cell division cycle 6 (CDC6) is an essential regulator of DNA replication and plays important roles in the activation and maintenance of the checkpoint mechanisms in the cell cycle. CDC6 has been associated with the oncogenic activities in human cancers, but the biological function and clinical significance of CDC6 in EOC remain unclear. The aim of the present study is to examine the effect of CDC6 on epithelial ovarian cancer (EOC) cells proliferation. We found that CDC6 protein level was up-regulated in EOC tissues compared with the normal ovary tissues. CDC6 expression correlated significantly with FIGO stage (plymph node status (pformation. HO8910 cells transfected with sh CDC6#1 underwent G1 phase cell cycle arrest. Collectively, this study provides a novel regulatory signaling pathway of CDC6-regulated EOC growth and a new potential therapeutic target for EOC patients. PMID:26920249

  15. Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization

    DEFF Research Database (Denmark)

    Petersen, B O; Lukas, J; Sørensen, Claus Storgaard;

    1999-01-01

    CDKs. CDC6 interacts specifically with the active Cyclin A/CDK2 complex in vitro and in vivo, but not with Cyclin E or Cyclin B kinase complexes. The cyclin binding domain of CDC6 was mapped to an N-terminal Cy-motif that is similar to the cyclin binding regions in p21(WAF1/SDI1) and E2F-1. The in vivo...... relocalizes to the cytoplasm when Cyclin A/CDK2 is activated. In agreement with CDC6 phosphorylation being specifically mediated by Cyclin A/CDK2, we show that ectopic expression of Cyclin A, but not of Cyclin E, leads to rapid relocalization of CDC6 from the nucleus to the cytoplasm. Based on our data we...... suggest that the phosphorylation of CDC6 by Cyclin A/CDK2 is a negative regulatory event that could be implicated in preventing re-replication during S phase and G2....

  16. Inhibition of CDC25B Phosphatase Through Disruption of Protein-Protein Interaction

    Energy Technology Data Exchange (ETDEWEB)

    Lund, George; Dudkin, Sergii; Borkin, Dmitry; Ni, Wendi; Grembecka, Jolanta; Cierpicki, Tomasz [Michigan

    2015-04-29

    CDC25 phosphatases are key cell cycle regulators and represent very attractive but challenging targets for anticancer drug discovery. Here, we explored whether fragment-based screening represents a valid approach to identify inhibitors of CDC25B. This resulted in identification of 2-fluoro-4-hydroxybenzonitrile, which directly binds to the catalytic domain of CDC25B. Interestingly, NMR data and the crystal structure demonstrate that this compound binds to the pocket distant from the active site and adjacent to the protein–protein interaction interface with CDK2/Cyclin A substrate. Furthermore, we developed a more potent analogue that disrupts CDC25B interaction with CDK2/Cyclin A and inhibits dephosphorylation of CDK2. Based on these studies, we provide a proof of concept that targeting CDC25 phosphatases by inhibiting their protein–protein interactions with CDK2/Cyclin A substrate represents a novel, viable opportunity to target this important class of enzymes.

  17. Blastomicose do sistema nervoso

    Directory of Open Access Journals (Sweden)

    Horácio M. Canelas

    1951-09-01

    Full Text Available Os autores chamam a atenção sôbre os erros de diagnóstico nos casos de neuroblastomicose, seja nas formas meningoencefálicas, seja nas tumorais. São focalizadas a paracoccidioidose e a criptococose. Depois de caracterizarem clìnicamente a paracoccidioidose, acentuam a raridade das formas nervosas (1,2% dos casos dessa micose autopsiados no Departamento de Anatomia Patológica da Faculdade de Medicina da Universidade de São Paulo. Pela revisão da literatura foi verificado haver apenas 12 casos registrados. 0 caso de Casiello e Klass é o único em que o diagnóstico foi feito em vida; os demais, ou constituem achado de necropsia, ou foram inesperadamente revelados pelo exame histopatológico realizado após intervenções cirúrgicas. Os autores registram 2 casos de paracoccidioidose do sistema nervoso. No primeiro, tratava-se de meningomielorra-diculite crônica, sendo o parasito identificado no escarro; o segundo era portador de síndrome de compressão medular, cuja patogenia é discutida pelos autores, tendo sido a etiologia paracoccidióidica comprovada pelo exame da polpa e biópsia ganglionares. Considerações clínicas sôbre a criptococose precedem o relato de um caso de meningoencefalomielite subaguda, no qual fôra feito em vida o diagnóstico de paracoccidioidose, pelo exame micológico do liqüido cefalorraqueano; êste paciente, embora medicado com doses maciças de sulfa e tiossemicarbarsona, veio a falecer, tendo o exame anátomo-patológico revelado tratar-se, na realidade, de lesões nervosas produzidas pelo Crypíococcus neo-formans. Nos três casos era incisivo o caráter neurocirúrgico da sintomatologia. Entretanto, ante o diagnóstico de paracoccidioidose, foi instituído tratamento pelas sulfas, vacina específica e tiossemicarbarsona. Os resultados foram excelentes no caso 2, em que a terapêutica pôde ser instituída mais precocemente; melhoras também foram obtidas no caso 1. No caso 3, foram verificadas

  18. Sistema de monitorización de sistemas distribuidos

    OpenAIRE

    Vilas Virulegio, Alberto

    2014-01-01

    La computación distribuida ha estado presente desde hace unos cuantos años, pero es quizás en la actualidad cuando está contando con una mayor repercusión. En los últimos años el modelo de computación en la nube (Cloud computing) ha ganado mucha popularidad, prueba de ello es la cantidad de productos existentes. Todo sistema informático requiere ser controlado a través de sistemas de monitorización que permiten conocer el estado del mismo, de tal manera que pueda ser gestionado fácilmente....

  19. Sistemas de tiempo real y sistemas distribuidos de tiempo real

    OpenAIRE

    Pesado, Patricia; Ramón, Hugo Dionisio; Boracchia, Marcos; Pasini, Ariel C.; De Vito, María Cecilia; Osella Massa, Germán Leandro; Estrebou, César

    2006-01-01

    El proyecto de investigación y desarrollo en Sistemas de Software Distribuido, abarca aspectos que van desde los fundamentos del desarrollo (Técnicas de Ingeniería de Requerimientos, Metodologías de Gestión y Desarrollo de Proyectos, Técnicas de Planificación, Métricas, Normas de Calidad, Web-Systems) hasta la concepción de aplicaciones específicas orientadas a los procesos industriales (Sistemas de Planeamiento de Producción, Control Industrial en tiempo real), procesos de E-government (Voto...

  20. IZGRADNJA INFORMACIJSKEGA SISTEMA ZA PODPORO SISTEMA RAZVOJA KADROV

    OpenAIRE

    Primožič, Živa

    2010-01-01

    Diplomsko delo obravnava uvedbo kadrovsko informacijskega sistema v podjetju, saj zaradi naraščanja števila zaposlenih vodenje evidence zaposlenih v papirni obliki predstavlja dolgoročno in zamudno delo z večjimi možnostmi za napake. V prvem delu je predstavljen problem ter na kratko podjetje Diss d.o.o. Sledi teoretična opredelitev razvoja kadrov ter njihov pomen. Osrednji del ter zaključek je namenjen rešitvi problema z uvedbo kadrovsko informacijskega sistema za podjetje Diss d.o.o. z ...

  1. The Ubx2 and Ubx3 cofactors direct Cdc48 activity to proteolytic and nonproteolytic ubiquitin-dependent processes

    DEFF Research Database (Denmark)

    Hartmann-Petersen, Rasmus; Wallace, Mairi; Hofmann, Kay;

    2004-01-01

    Valosin-containing protein, VCP/p97 or Cdc48, is a eukaryotic ATPase involved in membrane fusion, protein transport, and protein degradation. We describe two proteins, Ubx2 and Ubx3, which interact with Cdc48 in fission yeast. Ubx3 is the ortholog of p47/Shp1, a previously described Cdc48 cofactor...

  2. The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing

    DEFF Research Database (Denmark)

    Lischetti, Tiziana; Zhang, Gang; Sedgwick, Garry G; Bolanos-Garcia, Victor M; Nilsson, Jakob

    2014-01-01

    in the SAC, we delineate the molecular details of its interaction with kinetochores. We find that BubR1 recruits the bulk of Cdc20 to kinetochores through its internal Cdc20 binding domain (IC20BD). We show that preventing Cdc20 kinetochore localization by removal of the IC20BD has a limited effect...

  3. Exposiciones rábicas en Colombia: evaluación del sistema de vigilancia desde los actores/ Rabies exposures in Colombia: an evaluation by the actors of the surveillance system

    OpenAIRE

    Claudia P. Roncancio-Melgarejo; Daniel A. Buitrago-Medina; Isabel C. Posada-Zapata; Hugo Grisales-Romero

    2015-01-01

    Resumen Objetivo: identificar la percepción que tienen los actores frente a los atributos del sistema de vigilancia de las exposiciones rábicas para generar las recomendaciones que se requieran. Metodología: se utilizó la teoría fundada hasta análisis de categorías interpretativas; once entrevistas semiestructuradas enmarcadas en los atributos considerados por los Centros de Control y Prevención de Enfermedades (cdc) para la evaluación de sistemas de vigilancia se realizaron a personas con...

  4. Finite element dynamic analysis on CDC STAR-100 computer

    Science.gov (United States)

    Noor, A. K.; Lambiotte, J. J., Jr.

    1978-01-01

    Computational algorithms are presented for the finite element dynamic analysis of structures on the CDC STAR-100 computer. The spatial behavior is described using higher-order finite elements. The temporal behavior is approximated by using either the central difference explicit scheme or Newmark's implicit scheme. In each case the analysis is broken up into a number of basic macro-operations. Discussion is focused on the organization of the computation and the mode of storage of different arrays to take advantage of the STAR pipeline capability. The potential of the proposed algorithms is discussed and CPU times are given for performing the different macro-operations for a shell modeled by higher order composite shallow shell elements having 80 degrees of freedom.

  5. Announcing the CDC guideline for prescribing opioids for chronic pain.

    Science.gov (United States)

    Houry, Debra; Baldwin, Grant

    2016-06-01

    This guideline provides recommendations for primary care providers who are prescribing opioids for chronic pain outside of active cancer treatment, palliative care, and end-of-life care. The guideline addresses: (a) when to initiate or continue opioids for chronic pain; (b) opioid selection, dosage, duration, follow-up, and discontinuation; and (c) assessing risk and addressing harms of opioid use. This guideline is intended to improve communication between providers and patients about the risks and benefits of opioid therapy for chronic pain, improve the safety and effectiveness of pain treatment, and reduce the risks associated with long-term opioid therapy, including abuse, dependence, overdose, and death (Dowell D, Haegerich TM, Chou R. CDC Guideline for Prescribing Opioids for Chronic Pain - United States, 2016. MMWR Recomm Rep 2016;65:1-49. DOI: http://dx.doi.org/10.15585/mmwr.rr6501e1.). PMID:27178083

  6. Sorting on STAR. [CDC computer algorithm timing comparison

    Science.gov (United States)

    Stone, H. S.

    1978-01-01

    Timing comparisons are given for three sorting algorithms written for the CDC STAR computer. One algorithm is Hoare's (1962) Quicksort, which is the fastest or nearly the fastest sorting algorithm for most computers. A second algorithm is a vector version of Quicksort that takes advantage of the STAR's vector operations. The third algorithm is an adaptation of Batcher's (1968) sorting algorithm, which makes especially good use of vector operations but has a complexity of N(log N)-squared as compared with a complexity of N log N for the Quicksort algorithms. In spite of its worse complexity, Batcher's sorting algorithm is competitive with the serial version of Quicksort for vectors up to the largest that can be treated by STAR. Vector Quicksort outperforms the other two algorithms and is generally preferred. These results indicate that unusual instruction sets can introduce biases in program execution time that counter results predicted by worst-case asymptotic complexity analysis.

  7. Androgens upregulate Cdc25C protein by inhibiting its proteasomal and lysosomal degradation pathways.

    Directory of Open Access Journals (Sweden)

    Yu-Wei Chou

    Full Text Available Cdc25C is a cell cycle protein of the dual specificity phosphatase family essential for activating the cdk1/Cyclin B1 complex in cells entering into mitosis. Since altered cell cycle is a hallmark of human cancers, we investigated androgen regulation of Cdc25C protein in human prostate cancer (PCa cells, including androgen-sensitive (AS LNCaP C-33 cells and androgen-independent (AI LNCaP C-81 as well as PC-3 cells. In the regular culture condition containing fetal bovine serum (FBS, Cdc25C protein levels were similar in these PCa cells. In a steroid-reduced condition, Cdc25C protein was greatly decreased in AS C-33 cells but not AI C-81 or PC-3 cells. In androgen-treated C-33 cells, the Cdc25C protein level was greatly elevated, following a dose- and a time-dependent manner, correlating with increased cell proliferation. This androgen effect was blocked by Casodex, an androgen receptor blocker. Nevertheless, epidermal growth factor (EGF, a growth stimulator of PCa cells, could only increase Cdc25C protein level by about 1.5-fold. Altered expression of Cdc25C in C-33 cells and PC-3 cells by cDNA and/or shRNA transfection is associated with the corresponding changes of cell growth and Cyclin B1 protein level. Actinomycin D and cycloheximide could only partially block androgen-induced Cdc25C protein level. Treatments with both proteasomal and lysosomal inhibitors resulted in elevated Cdc25C protein levels. Immunoprecipitation revealed that androgens reduced the ubiquitination of Cdc25C proteins. These results show for the first time that Cdc25C protein plays a role in regulating PCa cell growth, and androgen treatments, but not EGF, greatly increase Cdc25C protein levels in AS PCa cells, which is in part by decreasing its degradation. These results can lead to advanced PCa therapy via up-regulating the degradation pathways of Cdc25C protein.

  8. Relaciones entre el derecho internacional y el derecho interno: Nuevas perspectivas doctrinales y jurisprudenciales en el ámbito americano

    Directory of Open Access Journals (Sweden)

    Aníbal Quiroga León

    2005-01-01

    Full Text Available El artículo analiza la protección de los derechos fundamentales en el marco de las relaciones entre derecho internacional y derecho interno, teniendo como punto central el sistema interamericano y sus órganos de aplicación, La Comisión y la Corte Interamericana de Derechos Humanos, considerando aspectos procedimentales y la adopción de medidas cautelares por esta última. Finalmente, se analiza la superioridad del derecho internacional de los derechos humanos sobre el derecho interno, teniendo como fundamento los casos "Barrios Altos" (Chumbipoma Aguirre y otros, cuya sentencia es del 14 de marzo del 2001 y el caso "La última tentación de Cristo" (Olmedo Bustos y otros cuya sentencia es del 05 de febrero del 2001.This article analyzes the protection of fundamental rights within the framework of the relationship between international and internal law. The core of our analysis is the Inter-American system, and its bodies of application: the Inter- American Human Rights Commission and Court. We consider procedural dimensions as well as precautionary measures pertaining to the latter. Finally, we examine the preeminence of the international law of human rights over internal law, on grounds of the "Barrios Altos" case (Chumbipoma Aguirre et al., decided on March, 2001 y the "La última tentación de Cristo" case (Olmedo Bustos et al. decided on February 5, 2001.

  9. Contêiner refrigerado: uma alternativa para a conservação pós-colheita de alface tipo americano Refrigerated container: an alternative for postharvest conservation

    Directory of Open Access Journals (Sweden)

    Bárbara J. Teruel

    2000-07-01

    Full Text Available A refrigeração é um excelente método para o armazenamento prolongado de produtos frescos. O uso de contêineres na agricultura é uma tecnologia simples e de fácil utilização que permite a manutenção da qualidade de produtos frescos, como frutas e hortaliças e a diminuição das perdas pós-colheita. Neste trabalho foram desenvolvidos uma série de experimentos com um contêiner frigorífico submetido a dois regimes de operação: vazio e com produto. Foram avaliadas as seguintes variáveis: temperatura, velocidade e umidade relativa do ar, além da pressão do sistema de refrigeração. Foi determinado o tempo de meio resfriamento de alface tipo americano (Latuca sativa L., submetido a uma temperatura de 1ºC. Nas superfícies que recebem maior radiação solar o desvio da temperatura é de 1,5 ± 1ºC, com respeito às paredes que recebem menor radiação. A velocidade do ar variou de 2,1; 1,7 e 0,3 m/s, nos três planos estudados. O tempo de meio resfriamento da carga de alface variou entre 530 min (» 8 h, e 1.080 min (» 18 h, dependendo da posição das cabeças de alface no interior do contêiner. A umidade relativa do ar foi de 81-86% ± 1% e de 90-91 ± 1%, para o contêiner vazio e com carga, respectivamente.Refrigeration is an excellent method for storing horticultural fresh products such as fruits and vegetables. The use of refrigerated containers is a simple and ready-to-use technology allowing better quality and decreasing postharvest losses. In this work some experiments were conducted with a refrigerated container in two operating conditions: with and without load. The following variables were evaluated: air temperature, air velocity, air relative humidity, and pressure of the refrigeration system. The lettuce (Latuca sativa L. half-cooling time was also determined when this product was stored at 1ºC. It was observed that the container surfaces receiving more radiation presented temperature increases of 1,5 ± 1ºC in

  10. Sistema de incendios inalámbrico

    OpenAIRE

    Menéndez Muñiz, Sergio

    2012-01-01

    Sistema de detección de incendios inalámbrico basado en dispositivos "motes" para la monitorización del estado del sistema, detección y reconocimiento de alarmas. Sistema de detecció d'incendis sense fils basat en dispositius "motes" per a la monitorització de l'estat del sistema, detecció i reconeixement d'alarmes.

  11. Sistemas de software distribuido : Aplicaciones

    OpenAIRE

    De Giusti, Armando Eduardo; Pesado, Patricia; Bertone, Rodolfo Alfredo; Boracchia, Marcos; Thomas, Pablo Javier; Madoz, María Cristina

    2002-01-01

    Realizar investigación y desarrollo en temas relacionados con Sistemas de Software Distribuido, particularmente los relacionados con los aspectos de Ingeniería de Software que se orientan al desarrollo e implementación de proyectos de sistemas distribuidos, incluyendo especialmente los temas de métricas y calidad de software. Estas tareas abarcan aspectos propios de la arquitectura, comunicaciones, estructuración de bases de datos, así como especificación, validación y desarrollo de aplica...

  12. Problemas actuales del sistema penitenciario

    OpenAIRE

    González Martín, Jennifer

    2014-01-01

    He pretendido con este proyecto analizar los cambios que actualmente está sufriendo nuestro sistema penitenciario desde una óptica meramente jurídica. A tal fin, este trabajo se centra en analizar, por una parte, el proyecto de reforma del Código Penal centrándose en los aspectos que afectan al sistema penitenciario (en el caso de que finalmente sea aprobado por las Cortes Generales), y, por otra parte, se estudia la conocida como "Doctrina Parot" analizando en qué consiste, cómo se implantó ...

  13. Un sistema de restricciones anotado

    OpenAIRE

    RAMÍREZ OVALLE, CARLOS ERNESTO

    2010-01-01

    En la programación concurrente con restricciones, el concepto básico es el de sistema de restricciones. Aquí presentamos una innovación sobre los sistemas de restricciones al introducir una lógica anotada como componente sintáctico y semántico junto con un motor de inferencia lógica en hiper-resolución anotada. Esto permite incrementar la capacidad expresiva de un modelo basado en satisfacción de restricciones pues es ahora posible incluir información adicional a las restricciones clásicas de...

  14. Sistema de coberta transitable ventilada

    OpenAIRE

    Pagés Serra, Jorge

    2013-01-01

    Investigació referent a l'aplicació pràctica d'un sistema constructiu per a realitzar una coberta ventilada transitable amb paviment continuu En el nostre clima la màxima insolació es produeix a la coberta. Per altre banda les cobertes a la catalana o cobertes ventilades, tant comuns a l'eixample i d'altres zones de barcelona han pràcticament desaparegut. Aquesta investigació preten desenvolupar un sistema constructiu que permeti la realització de cobertes ventilades amb paviments continus...

  15. Sistema de coordenadas para AR

    OpenAIRE

    Aguiar Cabello, Arnau

    2012-01-01

    Este proyecto realiza un estudio sobre las diferentes opciones referentes a la Realidad Aumentada, como primera parte. La Realidad Aumentada es una tecnología que en los últimos años ha experimentado un gran desarrollo. La Realidad Aumentada consiste en sobreponer información virtual con la ya existente realidad física. Como segunda parte, se escoge una de estas opciones, NyARToolkit, y se explica el funcionamiento del sistema de coordenadas basado en marcadores. El sistema basado en marca...

  16. El sistema tributario japonés

    OpenAIRE

    Yoshiyasu Okada

    2002-01-01

    Este documento primeramente trata las tendencias recientes y el estado actual del sistema tributario japonés como antecedentes. Luego resume los impuestos principales y describe el actual sistema. Finalmente trata los incentivos tributarios y los aspectos internacionales del sistema japonés.

  17. An Essential Role for Cdc42 in the Functioning of the Adult Mammary Gland.

    Science.gov (United States)

    Druso, Joseph E; Endo, Makoto; Lin, Miao-Chong Joy; Peng, Xu; Antonyak, Marc A; Meller, Stephanie; Cerione, Richard A

    2016-04-22

    The Rho family small GTPase Cdc42 has been implicated in a wide range of cellular functions including the establishment of cell polarity and the remodeling of the actin cytoskeletal architecture, resulting in the tight regulation of cell growth and survival during developmental processes. The complete knock-out of Cdc42 in the mouse is embryonic-lethal, and its targeted deletion in various tissues has been shown to disrupt tissue homeostasis. Thus far, in most studies, the targeted deletion of Cdc42 occurred during embryogenesis. Here, we have used a conditional gene deletion strategy in mice to probe the specific role of Cdc42 during adult mammary gland function. Cdc42 conditional-knock-out females were unable to adequately nourish their pups, due to a disorganized epithelial compartment within their mammary glands. A closer examination showed that their mammary epithelial cells were not able to maintain functional alveolar lumens, due to an inability to establish normal apical/basal epithelial polarity, as well as proper cell-cell contacts. Loss of these essential epithelial characteristics led to a premature sloughing off of the Cdc42-null epithelial cells. Overall our findings demonstrate that Cdc42 plays essential roles in mammary gland function post pregnancy, where it helps to establish proper epithelial cell polarity and tissue homeostasis during lactation. PMID:26912661

  18. Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes

    Science.gov (United States)

    Lange, Bodo M.H.; Rebollo, Elena; Herold, Andrea; González, Cayetano

    2002-01-01

    Cdc37 has been shown to be required for the activity and stability of protein kinases that regulate different stages of cell cycle progression. However, little is known so far regarding interactions of Cdc37 with kinases that play a role in cell division. Here we show that the loss of function of Cdc37 in Drosophila leads to defects in mitosis and male meiosis, and that these phenotypes closely resemble those brought about by the inactivation of Aurora B. We provide evidence that Aurora B interacts with and requires the Cdc37/Hsp90 complex for its stability. We conclude that the Cdc37/Hsp90 complex modulates the function of Aurora B and that most of the phenotypes brought about by the loss of Cdc37 function can be explained by the inactivation of this kinase. These observations substantiate the role of Cdc37 as an upstream regulatory element of key cell cycle kinases. PMID:12374737

  19. Opposing Roles for Actin in Cdc42p PolarizationD⃞

    Science.gov (United States)

    Irazoqui, Javier E.; Howell, Audrey S.; Theesfeld, Chandra L.; Lew, Daniel J.

    2005-01-01

    In animal and fungal cells, the monomeric GTPase Cdc42p is a key regulator of cell polarity that itself exhibits a polarized distribution in asymmetric cells. Previous work showed that in budding yeast, Cdc42p polarization is unaffected by depolymerization of the actin cytoskeleton (Ayscough et al., J. Cell Biol. 137, 399–416, 1997). Surprisingly, we now report that unlike complete actin depolymerization, partial actin depolymerization leads to the dispersal of Cdc42p from the polarization site in unbudded cells. We provide evidence that dispersal is due to endocytosis associated with cortical actin patches and that actin cables are required to counteract the dispersal and maintain Cdc42p polarity. Thus, although Cdc42p is initially polarized in an actin-independent manner, maintaining that polarity may involve a reinforcing feedback between Cdc42p and polarized actin cables to counteract the dispersing effects of actin-dependent endocytosis. In addition, we report that once a bud has formed, polarized Cdc42p becomes more resistant to dispersal, revealing an unexpected difference between unbudded and budded cells in the organization of the polarization site. PMID:15616194

  20. Cdc6 is a rate-limiting factor for proliferative capacity during HL60 cell differentiation

    International Nuclear Information System (INIS)

    The DNA replication (or origin) licensing pathway represents a critical step in cell proliferation control downstream of growth signalling pathways. Repression of origin licensing through down-regulation of the MCM licensing factors (Mcm2-7) is emerging as a ubiquitous route for lowering proliferative capacity as metazoan cells exit the cell division cycle into quiescent, terminally differentiated and senescent 'out-of-cycle' states. Using the HL60 monocyte/macrophage differentiation model system and a cell-free DNA replication assay, we have undertaken direct biochemical investigations of the coupling of origin licensing to the differentiation process. Our data show that down-regulation of the MCM loading factor Cdc6 acts as a molecular switch that triggers loss of proliferative capacity during early engagement of the somatic differentiation programme. Consequently, addition of recombinant Cdc6 protein to in vitro replication reactions restores DNA replication competence in nuclei prepared from differentiating cells. Differentiating HL60 cells over-expressing either wild-type Cdc6 or a CDK phosphorylation-resistant Cdc6 mutant protein (Cdc6A4) exhibit an extended period of cell proliferation compared to mock-infected cells. Notably, differentiating HL60 cells over-expressing the Cdc6A4 mutant fail to down-regulate Cdc6 protein levels, suggesting that CDK phosphorylation of Cdc6 is linked to its down-regulation during differentiation and the concomitant decrease in cell proliferation. In this experimental model, Cdc6 therefore plays a key role in the sequential molecular events leading to repression of origin licensing and loss of proliferative capacity during execution of the differentiation programme

  1. Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei

    International Nuclear Information System (INIS)

    Highlights: • Cd2+ induces Cdc42 and MAPKs pathway related gene of Litopenaeus vannamei up-regulation. • Reduction of THC, increase of ROS production and apoptotic cell rate were observed when the shrimps exposure to Cd2+. • DsRNA-suppression of LvCdc42 and MAPKs during Cd2+ stress reduces the ROS production and apoptosis. • We conclude that LvCdc42 and MAPKs play key roles in Cd2+ stress responses of shrimps. - Abstract: Cadmium, one of the most toxic heavy metals in aquatic environments, has severe effects on marine invertebrates and fishes. The MAPK signaling pathway plays a vital role in stress responses of animals. The mitogen-activated protein kinase (MAPK) signaling pathway plays a vital role in animals’ stress responses, including mediation of apoptosis induced by the Rho GTPase Cdc42. However, there is limited knowledge about its function in shrimps, although disorders exacerbated by environmental stresses (including heavy metal pollution) have caused serious mortality in commercially cultured shrimps. Thus, we probed roles of Cdc42 in Litopenaeus vannamei shrimps (LvCdc42) during cadmium exposure by inhibiting its expression using dsRNA-mediated RNA interference. The treatment successfully reduced expression levels of MAPKs (including p38, JNK, and ERK). Cadmium exposure induced significant increases in expression levels of LvCdc42 and MAPKs, accompanied by reductions in total hemocyte counts (THC) and increases in apoptotic hemocyte ratios and ROS production. However, all of these responses were much weaker in LvCdc42-suppressed shrimps, in which mortality rates were higher than in controls. Our results suggest that the MAPK pathway plays a vital role in shrimps’ responses to Cd2+. They also indicate that LvCdc42 in shrimps participates in its regulation, and thus plays key roles in ROS production, regulation of apoptosis and associated stress responses

  2. Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Ting; Wang, Wei-Na, E-mail: weina63@aliyun.com; Gu, Mei-Mei; Xie, Chen-Ying; Xiao, Yu-Chao; Liu, Yuan; Wang, Lei

    2015-06-15

    Highlights: • Cd{sup 2+} induces Cdc42 and MAPKs pathway related gene of Litopenaeus vannamei up-regulation. • Reduction of THC, increase of ROS production and apoptotic cell rate were observed when the shrimps exposure to Cd{sup 2+}. • DsRNA-suppression of LvCdc42 and MAPKs during Cd{sup 2+} stress reduces the ROS production and apoptosis. • We conclude that LvCdc42 and MAPKs play key roles in Cd{sup 2+} stress responses of shrimps. - Abstract: Cadmium, one of the most toxic heavy metals in aquatic environments, has severe effects on marine invertebrates and fishes. The MAPK signaling pathway plays a vital role in stress responses of animals. The mitogen-activated protein kinase (MAPK) signaling pathway plays a vital role in animals’ stress responses, including mediation of apoptosis induced by the Rho GTPase Cdc42. However, there is limited knowledge about its function in shrimps, although disorders exacerbated by environmental stresses (including heavy metal pollution) have caused serious mortality in commercially cultured shrimps. Thus, we probed roles of Cdc42 in Litopenaeus vannamei shrimps (LvCdc42) during cadmium exposure by inhibiting its expression using dsRNA-mediated RNA interference. The treatment successfully reduced expression levels of MAPKs (including p38, JNK, and ERK). Cadmium exposure induced significant increases in expression levels of LvCdc42 and MAPKs, accompanied by reductions in total hemocyte counts (THC) and increases in apoptotic hemocyte ratios and ROS production. However, all of these responses were much weaker in LvCdc42-suppressed shrimps, in which mortality rates were higher than in controls. Our results suggest that the MAPK pathway plays a vital role in shrimps’ responses to Cd{sup 2+}. They also indicate that LvCdc42 in shrimps participates in its regulation, and thus plays key roles in ROS production, regulation of apoptosis and associated stress responses.

  3. O Crisol: periódico das alunas do Colégio Americano (Porto Alegre/RS, 1945-1964 - O Crisol: the jornal by students of the Colégio Americano (Porto Alegre/RS, 1945-1964

    Directory of Open Access Journals (Sweden)

    Dóris Bittencourt Almeida, Brasil

    2013-04-01

    Full Text Available Neste trabalho investiga-se o periódico O Crisol, produzido pelas alunas do Colégio Americano, uma escola de Porto Alegre/RS, entre os anos de 1945 e 1964. Esse periódicodiz muito à História da Educação, pois evidencia saberes e práticas que circulavam nacomunidade do Colégio e revela um pouco das representações da cultura juvenil daquele tempo. Aqui interessam os significados dos discursos difundidos pelo periódico nos processos de subjetivação provocados pelos textos e suas possíveis influências na construção das identidades das jovens leitoras e escritoras do jornal. A pesquisa identifica-se com os pressupostos teóricos da História Cultural e se inscreve no campo das práticas de leitura e escrita e de memórias juvenis, tendo como referenciais as concepções da cultura escrita enquanto produção discursiva de um determinado tempo e lugar.Palavras-chave: memórias juvenis, escritas escolares, práticas de escrita. O CRISOL: THE JORNAL BY STUDENTS OF THE COLÉGIO AMERICANO (PORTO ALEGRE/RS, 1945-1964 AbstractThis work investigates the journalO Crisolproduced by students of the Colégio Americano, a school in Porto Alegre/RS, between the years 1945 and 1964.The journal says much to the History of Education, as evidenced knowledges and pratices circulating in the community,showing a bit of representations of youth culture in that time. Herethe interest by meaning’s dissemination of speeches by the journal in the processes of subjectivation caused by texts and their possible influences on the construction of identities of young readers and writers of the newspaper. The research identifies with the theoreticals presuppositions o Cultural History and inscribed in the field of reading and writing practices and memories of youth, taking as reference the concepts of the writing culture as a discursive production of a determinated time and place.Key-words: youth’s memories, written school, writing practices. O CRISOL: EL

  4. Cytoplasmic Localization of Human cdc25C during Interphase Requires an Intact 14-3-3 Binding Site

    OpenAIRE

    Dalal, Sorab N.; Schweitzer, Colleen M.; Gan, Jianmin; DeCaprio, James A.

    1999-01-01

    cdc25C induces mitosis by activating the cdc2-cyclin B complex. The intracellular localization of cyclin B1 is regulated in a cell cycle-specific manner, and its entry into the nucleus may be required for the initiation of mitosis. To determine the cellular localization of cdc25C, monoclonal antibodies specific for cdc25C were developed and used to demonstrate that in human cells, cdc25C is retained in the cytoplasm during interphase. A deletion analysis identified a 58-amino-acid region (ami...

  5. DO PACTO NACIONAL À GLOBALIZAÇÃO: ESTADO E SINDICATO NA REGULAÇÃO DO CAPITALISMO NORTE-AMERICANO

    Directory of Open Access Journals (Sweden)

    Flavio Limoncic

    2007-09-01

    Full Text Available In the 1930s, the State and the unions in the United States begun a pro-cess of deep transformations that would allow them, in the next decades, to play central roles in the regulation of the American capitalism. However, both of them were unable to build permanent institutions of social agreement, like a labor party or tripartite agencies. Therefore, the North-american keynesian fordism resulted less institucionalized than the European social-democrat ones and, in the 1960s, not the unions, but the civil rights movements, would play a central role in the American political arena. With the crisis of the keynesian fordism and the corpora-te and conservative offensive of the 1970s, along with the beginning of the globa-lization process, which weakened nationally circumscribed social agreements, American unions went through a process of defensivness, in which the fight for material gains were substituted by the maintenance of inadequate jobs. Na década de 1930, o sindicalismo e o Estado norte-americanos inicia-ram um processo de profundas transformações que os permitiria acumular recur-sos políticos para, nas décadas seguintes, constituirem-se como atores centrais na regulação do capitalismo dos Estados Unidos. No entanto, ambos revelaram-se incapazes de traduzir esta centralidade em instituições permanentes de pactuação social. Por um lado, apesar de tema recorrente em sua agenda, o movimento sindical jamais conseguiu construir um partido trabalhista, e suas relações com o Partido Democrata nunca foram plenamente orgânicas; de outro, a constituição de agências tripartites para a construção de macro-acordos sociais revelou-se inviável com a reação conservadora do pós-Guerra . O pacto fordista keynesiano norte-americano, portanto, revelou-se menos institucionalizado que seus congê-neres social-democratas europeus. Na década de 1960, não seriam mais os sindi-catos, mas os movimentos pela defesa dos direitos civis, que

  6. NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling

    DEFF Research Database (Denmark)

    Sørensen, Claus Storgaard; Melixetian, Marina; Klein, Ditte Kjaersgaard;

    2010-01-01

    The DNA damage induced G(2)/M checkpoint is an important guardian of the genome that prevents cell division when DNA lesions are present. The checkpoint prevents cells from entering mitosis by degrading CDC25A, a key CDK activator. CDC25A proteolysis is controlled by direct phosphorylation events...... required for beta-TrCP mediated CDC25A polyubiquitylation and degradation. The activity of NEK11 is in turn controlled by CHK1 that activates NEK11 via phosphorylation on serine 273. Since inhibition of NEK11 activity forces checkpoint-arrested cells into mitosis and cell death, NEK11 is, like CHK1, a...

  7. Cdc20 mediates D-box-dependent degradation of Sp100

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Ran; Li, Ke-min; Zhou, Cai-hong; Xue, Jing-lun [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China); Ji, Chao-neng, E-mail: Chnji@fudan.edu.cn [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China); Chen, Jin-zhong, E-mail: kingbellchen@fudan.edu.cn [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China)

    2011-12-02

    Highlights: Black-Right-Pointing-Pointer Cdc20 is a co-activator of APC/C complex. Black-Right-Pointing-Pointer Cdc20 recruits Sp100 and mediates its degradation. Black-Right-Pointing-Pointer The D-box of Sp100 is required for Cdc20-mediated degradation. Black-Right-Pointing-Pointer Sp100 expresses consistently at both the mRNA and protein levels in cell cycle. -- Abstract: Cdc20 is a co-activator of the anaphase-promoting complex/cyclosome (APC/C complex), which recruits substrates at particular phases of the cell cycle and mediates their degradation. Sp100 is a PML-NB scaffold protein, which localizes to nuclear particles during interphase and disperses from them during mitosis, participates in viral resistance, transcriptional regulation, and apoptosis. However, its metabolism during the cell cycle has not yet been fully characterized. We found a putative D-box in Sp100 using the Eukaryotic Linear Motif (ELM) predictor database. The putative D-box of Sp100 was verified by mutational analysis. Overexpression of Cdc20 resulted in decreased levels of both endogenous Sp100 protein and overexpressed Sp100 mRNA in HEK 293 cells. Only an overexpressed D-box deletion mutant of Sp100 accumulated in HEK293 cells that also overexpressed Cdc20. Cdc20 knockdown by cdc20 specific siRNA resulted in increased Sp100 protein levels in cells. Furthermore, we discovered that the Cdc20 mediated degradation of Sp100 is diminished by the proteasome inhibitor MG132, which suggests that the ubiquitination pathway is involved in this process. However, unlike the other Cdc20 substrates, which display oscillating protein levels, the level of Sp100 protein remains constant throughout the cell cycle. Additionally, both overexpression and knockdown of endogenous Sp100 had no effect on the cell cycle. Our results suggested that sp100 is a novel substrate of Cdc20 and it is degraded by the ubiquitination pathway. The intact D-box of Sp100 was necessary for this process. These findings expand

  8. CDC25A governs proliferation and differentiation of FLT3-ITD acute myeloid leukemia

    OpenAIRE

    Bertoli, Sarah; Boutzen, Helena; David, Laure; Larrue, Clément; Vergez, François; Fernandez-Vidal, Anne; Yuan, Lingli; Hospital, Marie-Anne; Tamburini, Jérôme; Demur, Cecile; Delabesse, Eric; Saland, Estelle; Sarry, Jean-Emmanuel; Galcera, Marie-Odile; Mansat-De Mas, Véronique

    2015-01-01

    We investigated cell cycle regulation in acute myeloid leukemia cells expressing the FLT3-ITD mutated tyrosine kinase receptor, an underexplored field in this disease. Upon FLT3 inhibition, CDC25A mRNA and protein were rapidly down-regulated, while levels of other cell cycle proteins remained unchanged. This regulation was dependent on STAT5, arguing for FLT3-ITD-dependent transcriptional regulation of CDC25A. CDC25 inhibitors triggered proliferation arrest and cell death of FLT3-ITD as well ...

  9. Knowledge Exchange with Sistema Scotland

    Science.gov (United States)

    Allan, Julie; Moran, Nikki; Duffy, Celia; Loening, Gica

    2010-01-01

    This paper reports on a knowledge exchange project, funded by the Scottish Funding Council and undertaken by a group of researchers from three higher education institutions in Scotland and the project partner, Sistema Scotland. This newly established charity is attempting to implement a major programme of social change, developed in Venezuela,…

  10. Thoughts on Seeing "El Sistema"

    Science.gov (United States)

    Booth, Eric

    2009-01-01

    The FESNOJIV (the Spanish acronym for the State Foundation for the National System of Youth and Children's Orchestras), more commonly known as "El Sistema," teaches 300,000 of Venezuela's poorest children in the nationwide music learning program. Many begin attending the "nucleo" as early as age two or three, and the vast majority continue well…

  11. Metodologia de sistemas de data warehouse

    OpenAIRE

    Sá, Jorge Vaz de Oliveira e

    2010-01-01

    Tese de doutoramento em Tecnologias e Sistemas de Informação (área de conhecimento em Engenharia e Gestão de Sistemas de Informação) Os sistemas de Data Warehouse possibilitam que as organizações tenham acesso a informação de gestão que é essencial para a vida da organização. No entanto a implementação destes sistemas nas organizações sofre de uma elevada taxa de insucesso. Várias iniciativas de implementação de Sistemas de Data Warehouse arrancaram com grandes expectativas, mas r...

  12. El visón americano Neovison vison (Schreber 1777 en España: posibles causas de su expansión e interacción con otros mamíferos semiacuáticos

    Directory of Open Access Journals (Sweden)

    A. Balmori

    2015-01-01

    Full Text Available El establecimiento de poblaciones de visón americano asilvestradas en España data de la década de los 60 del siglo XX. Los individuos procedentes de escapes de granjas peleteras han establecido poblaciones asilvestradas que se han expandido rápidamente por la cuenca del Duero. Tanto los motivos de su exitosa expansión en la submeseta norte como los efectos en la comunidad de vertebrados ligados a los ríos son poco conocidos. En este trabajo se revisan las posibles causas del éxito colonizador del visón americano en España y el previsible impacto sobre las especies de mamíferos semiacuáticos con las que comparte territorio, con especial mención de aquellos cuyas poblaciones pueden verse amenazadas. Se proponen las actuaciones necesarias para favorecer la recuperación de las poblaciones de visón europeo y frenar la expansión del visón americano en la Cuenca del Duero. La evolución de la superficie de ocupación del visón europeo permite albergar cierto optimismo.

  13. Las virtudes del sistema binominal

    Directory of Open Access Journals (Sweden)

    John M Carey

    2006-01-01

    Full Text Available El sistema único de elecciones legislativas chileno, en el que cada distrito elige a dos representantes (M=2, ha sido objeto de controversia y de propuestas de reforma, ya que fue impuesto por el régimen autoritario saliente en 1990. La mayor parte de ellas aboga por la llamada mayor proporcionalidad. Este ensayo sostiene que, a pesar de sus orígenes no democráticos, el sistema M=2 ha funcionado relativamente bien respecto a los sistemas empleados en muchos otros países. En particular, M=2 en Chile entrega una mezcla adecuada de representatividad, gobernabilidad y responsabilidad individual del legislador. El ensayo considera varias alternativas para la reforma y perfila algunos problemas potenciales que cada una implicaría. Si se reforma el sistema chileno, entonces el autor recomienda que se haga por pasos incrementales, no fundamentales -un aumento moderado en la magnitud de cada distrito-, pero manteniendo las listas abiertas y no haciendo el salto a un sistema mixto de miembros (SMMChile's unique system of legislative elections, in which each district elects two representatives (M=2, has been subject to controversy and a target for reform proposals since it was imposed on the country by the outgoing authoritarian regime in 1990. Most reform advocates call for greater proportionality. This essay argues that, desipte its undemocratic origins, the M=2 system has performed quite well relative to systems used in many other countries. In particular, M=2 in Chile delivers a reasonable mix of representativeness, governability, and individual legislator accountability. The essay goes on to consider various alternatives for reform and outlines some potential problems each would entail. If the Chilean system is to be reformed, the essay recommends incrementalism - a moderate increase in district magnitude, perhaps, but maintaining open lists, and no change to a mixed-member system (MMS

  14. As contribuições do II Congresso Médico Latino-Americano para a enfermagem no Brasil Las Contribuciones del II Congreso Médico Latino-Americano para la enfermería en el Brasil The contributions of II Latin American Medical Congress for the Nursing in Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda Teles Morais

    2009-03-01

    Full Text Available Estudo sobre as recomendações do II Congresso Médico Latino-Americano, em 1904, para a organização da Enfermagem no Brasil. Objetivos: Caracterizar os médicos brasileiros que participaram do II Congresso Médico Latino-Americano e analisar as recomendações deste Congresso para a Enfermagem. Trata-se de um estudo histórico-social, embasado na análise documental. Resultados: A participação de médicos brasileiros neste evento foi grande. Em meio às discussões de ordem sanitária, figurou a questão da criação de escolas de enfermeiras, apresentada pelo médico Moisés Amaral. Diante das evidências da eficácia de se ter uma enfermeira instruída em escolas próprias, e de acordo com os preceitos da medicina moderna, foi aprovada a proposta para a criação de escolas de enfermagem. Apesar da posição estratégica que os representantes brasileiros nesse Congresso ocupavam no país, não encontramos evidências do empenho deles em trabalhar efetivamente para a criação de escolas de enfermeiras como uma estratégia para enfrentar os problemas sanitários.Estudio sobre las recomendaciones del II Congreso Médico Latino-Americano, en 1904, para la organización de la enfermería el Brasil. Objetivos: Caracterizar los médicos brasileños que participaran del II Congreso Médico Latino-Americano; y analizar las recomendaciones de este congreso para la enfermería. Estudio histórico-social basado en la análisis documental. Resultados: la participación brasileña en este acontecimiento foi grande. En medio a las discusiones sanitarias, figuró la cuestión de creación de escuelas de enfermeríapresentada por el médico Moisés Amaral. Frente a las evidencias de la eficacia de tener una enfermera instruida en escuelas apropiadas y de acuerdo con los preceptos de la medicina moderna fue aprobada la propuesta de creación de escuelas de enfermería. A pesar de la estratégica posición de los representantes brasileños en ese Congreso

  15. Characterization of a Dual CDC7/CDK9 Inhibitor in Multiple Myeloma Cellular Models

    International Nuclear Information System (INIS)

    Two key features of myeloma cells are the deregulation of the cell cycle and the dependency on the expression of the BCL2 family of anti-apoptotic proteins. The cell division cycle 7 (CDC7) is an essential S-phase kinase and emerging CDC7 inhibitors are effective in a variety of preclinical cancer models. These compounds also inhibit CDK9 which is relevant for MCL-1 expression. The activity and mechanism of action of the dual CDC7/CDK9 inhibitor PHA-767491 was assessed in a panel of multiple myeloma cell lines, in primary samples from patients, in the presence of stromal cells and in combination with drugs used in current chemotherapeutic regimens. We report that in all conditions myeloma cells undergo cell death upon PHA-767491 treatment and we report an overall additive effect with melphalan, bortezomib and doxorubicin, thus supporting further assessment of targeting CDC7 and CDK9 in multiple myeloma

  16. Businesses Should Be Mindful of Zika Danger to Workers, CDC Says

    Science.gov (United States)

    ... nih.gov/medlineplus/news/fullstory_158458.html Businesses Should Be Mindful of Zika Danger to Workers, CDC ... a news release. As for travel protocols, "employers should consider allowing flexibility in required travel to areas ...

  17. 75 FR 39264 - CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment

    Science.gov (United States)

    2010-07-08

    ... CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment In accordance with section 10(a)(2..., Viral Hepatitis, STD, and TB ] Prevention, 1600 Clifton Road, NE., Mailstop E-07, Atlanta, Georgia...

  18. 76 FR 66721 - CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment

    Science.gov (United States)

    2011-10-27

    ... Prevention and Treatment In accordance with section l0(a)(2) of the Federal Advisory Committee Act (Pub. L...-Cseh, CDC, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, 1600 Clifton Road,...

  19. Characterization of a Dual CDC7/CDK9 Inhibitor in Multiple Myeloma Cellular Models

    Energy Technology Data Exchange (ETDEWEB)

    Natoni, Alessandro [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Coyne, Mark R. E. [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Department of Medicine, National University of Ireland Galway, Galway (Ireland); Department of Haematology, Galway University Hospital, Galway (Ireland); Jacobsen, Alan; Rainey, Michael D.; O’Brien, Gemma; Healy, Sandra [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Montagnoli, Alessia; Moll, Jürgen [Nerviano Medical Sciences S.r.l., Via Pasteur 10, Nerviano 20014 (Italy); O’Dwyer, Michael, E-mail: michael.odwyer@nuigalway.ie [Department of Medicine, National University of Ireland Galway, Galway (Ireland); Department of Haematology, Galway University Hospital, Galway (Ireland); Santocanale, Corrado, E-mail: michael.odwyer@nuigalway.ie [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland)

    2013-07-24

    Two key features of myeloma cells are the deregulation of the cell cycle and the dependency on the expression of the BCL2 family of anti-apoptotic proteins. The cell division cycle 7 (CDC7) is an essential S-phase kinase and emerging CDC7 inhibitors are effective in a variety of preclinical cancer models. These compounds also inhibit CDK9 which is relevant for MCL-1 expression. The activity and mechanism of action of the dual CDC7/CDK9 inhibitor PHA-767491 was assessed in a panel of multiple myeloma cell lines, in primary samples from patients, in the presence of stromal cells and in combination with drugs used in current chemotherapeutic regimens. We report that in all conditions myeloma cells undergo cell death upon PHA-767491 treatment and we report an overall additive effect with melphalan, bortezomib and doxorubicin, thus supporting further assessment of targeting CDC7 and CDK9 in multiple myeloma.

  20. Defective tubulin organization and proplatelet formation in murine megakaryocytes lacking Rac1 and Cdc42

    DEFF Research Database (Denmark)

    Pleines, Irina; Dütting, Sebastian; Cherpokova, Deya; Eckly, Anita; Meyer, Imke; Morowski, Martina; Krohne, Georg; Schulze, Harald; Gachet, Christian; Debili, Najet; Brakebusch, Cord Herbert; Nieswandt, Bernhard

    2013-01-01

    and tubulin cytoskeleton. Rho GTPases, such as RhoA, Rac1, and Cdc42, are important regulators of cytoskeletal rearrangements in platelets; however, the specific roles of these proteins during platelet production have not been established. Using conditional knockout mice, we show here that Rac1 and...... Cdc42 possess redundant functions in platelet production and function. In contrast to a single-deficiency of either protein, a double-deficiency of Rac1 and Cdc42 in MKs resulted in macrothrombocytopenia, abnormal platelet morphology, and impaired platelet function. Double-deficient bone marrow MKs...... matured normally in vivo but displayed highly abnormal morphology and uncontrolled fragmentation. Consistently, a lack of Rac1/Cdc42 virtually abrogated proplatelet formation in vitro. Strikingly, this phenotype was associated with severely defective tubulin organization, whereas actin assembly and...

  1. 13 CFR 120.972 - Third Party Lender participation fee and CDC fee.

    Science.gov (United States)

    2010-01-01

    ... BUSINESS LOANS Development Company Loan Program (504) Fees § 120.972 Third Party Lender participation fee... when the Third Party Lender occupies a senior credit position to SBA in the Project. (b) CDC fee....

  2. Working at a Ferranti-Argus graphics display linked to a CDC 6600

    CERN Multimedia

    1975-01-01

    The photo shows a Ferranti-Argus graphics display linked to a CDC 6600 computer running one of the first interactive graphics packages for physics analysis. The people around are Jean-Claude Marin, Harry Renshall and Emilio Pagiola (right).

  3. Breastfeeding Among U.S. Children Born 2000 - 2009, CDC National Immunization Survey

    Science.gov (United States)

    ... Their Families Communities Health Care Employment Research & Surveillance Public Health Infrastructure National Policies & Positions Healthy People 2020 Frequently Asked Questions (FAQs) Resources & Publications Breastfeeding Progress MMWR Highlights CDC Guide to Strategies to ...

  4. Fewer U.S. Kids Die from Abusive Head Trauma: CDC

    Science.gov (United States)

    ... https://medlineplus.gov/news/fullstory_159052.html Fewer U.S. Kids Die From Abusive Head Trauma: CDC Parent ... two years of the study, according to the U.S. Centers for Disease Control and Prevention. Experts said ...

  5. U.S. Suicide Rate Up 24 Percent Since 1999: CDC

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_158462.html U.S. Suicide Rate Up 24 Percent Since 1999: CDC ... per 100,000 people, said researchers from the U.S. Centers for Disease Control and Prevention's National Center ...

  6. U.S. Autism Rate Unchanged at 1 in 68 Kids: CDC

    Science.gov (United States)

    ... nih.gov/medlineplus/news/fullstory_158064.html U.S. Autism Rate Unchanged at 1 in 68 Kids: CDC ... 2016 THURSDAY, March 31, 2016 (HealthDay News) -- The autism rate among school-aged children in the United ...

  7. SIMWEST: A simulation model for wind and photovoltaic energy storage systems (CDC user's manual), volume 1

    Science.gov (United States)

    Warren, A. W.; Esinger, A. W.

    1979-01-01

    Procedures are given for using the SIMWEST program on CDC 6000 series computers. This expanded software package includes wind and/or photovoltaic systems utilizing any combination of five types of storage (pumped hydro, battery, thermal, flywheel, and pneumatic).

  8. CDC Zika Summit Details Plans to Fight Mosquito-Borne Illness

    Science.gov (United States)

    ... nih.gov/medlineplus/news/fullstory_158094.html CDC Zika Summit Details Plans to Fight Mosquito-Borne Illness ... the continental United States braces itself for the Zika virus, federal, state and local health officials gathered ...

  9. U.S. Suicide Rate Up 24 Percent Since 1999: CDC

    Science.gov (United States)

    ... nih.gov/medlineplus/news/fullstory_158462.html U.S. Suicide Rate Up 24 Percent Since 1999: CDC Rates ... 22, 2016 FRIDAY, April 22, 2016 (HealthDay News) -- Suicide rates in the United States rose 24 percent ...

  10. 78 FR 60876 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2013-10-02

    ... the next day. Number for Technical Support: (404) 639-3737 The deadline for notification of attendance...: Carmen Villar, MSW, Designated Federal Officer, Advisory Committee to the Director, CDC, 1600...

  11. O pensamento conservador ibero-americano na era das independências (1808-1850 Conservative ibero-american thought in the age of independences (1808-1850

    Directory of Open Access Journals (Sweden)

    Christian Edward Cyril Lynch

    2008-01-01

    Full Text Available A intenção do artigo é fornecer um panorama ideológico da América Ibérica da época das independências, especialmente no que diz respeito às ideologias de direita. Busca-se, assim, definir o que seja conservadorismo, distinguindo-o do puro reacionarismo para, em seguida, comparar a orientação de José Bonifácio e de Marquês de Caravelas, no Brasil, com aquela desenvolvida por Bolívar, tendo em vista os dilemas do conservadorismo ibero-americano. A comparação esclarece as diferentes trajetórias da antiga América portuguesa e da antiga América hispânica, no decorrer do século XIX.This article intends to study the right-wing ideologies of Latin America during the first half of the nineteenth century. At first, it tries to define what conservatism is and to point out its differences from the rhetoric of reaction; later, the thought of José Bonifacio and the Marquis of Caravelas is compared to that of Simon Bolivar, underlying the dilemmas of Latin American conservatism. The comparison enlightens the different ways followed by the ancient Portuguese and Hispanic America after political independence.

  12. "The Americano Dream" ou "The American Dream": o debate contemporâneo sobre a imigração de mexicanos para os Estados Unidos

    Directory of Open Access Journals (Sweden)

    Celso Antônio Coelho Vaz

    2014-12-01

    Full Text Available Neste artigo resenho o debate acadêmico suscitado nos Estados Unidos por causa dos argumentos de Huntington sobre a imigração hispânica, sobretudo a de mexicanos, para este país. Em suas obras "The Hispanic challenge" e Who are we?, Huntington argumenta que, por razões demográficas e políticas, os imigrantes mexicanos não se assimilariam ao core culture norte-americano fundado nos valores anglo-protestantes, o que dividiria os EUA em dois povos, duas culturas e duas linguagens. As pesquisas empíricas aqui resenhadas que testaram os argumentos empíricos de Huntington os negaram e os críticos teóricos destes concebem que eles são doutrinários porque expressam uma ideologia settler, bem como manifestam a teoria do conflito do pluralismo cultural e a do colonianismo interno.

  13. Nodulação em cultivares de feijão dos conjuntos gênicos andino e meso-americano

    Directory of Open Access Journals (Sweden)

    Franco Marília Caixeta

    2002-01-01

    Full Text Available O objetivo deste trabalho foi determinar a capacidade de nodulação e a especificidade de feijão (Phaseolus vulgaris L. dos conjuntos gênicos andino e meso-americano submetidos a inóculo de Rhizobium. O experimento foi estabelecido em parcelas subdivididas em blocos ao acaso, com quatorze cultivares de feijão e três estirpes de Rhizobium (R. etli KIM 5, R. etli CIAT 632 e R. tropici CIAT 899. Somente as cultivares andinas WAF 15, WAF 7, Mineiro Precoce, WAF 6 e Antioquia 8 apresentaram especificidade na nodulação. Em relação à massa seca dos nódulos, houve diferenças significativas dos tratamentos de inoculação nas cultivares andinas WAF 15, WAF 7, WAF 6 e Diacol Andino, e na cultivar meso-americana Ouro Negro. Nenhuma das cultivares restringiu a nodulação, embora tenham sido verificadas diferenças de até 53 e 103 vezes no número e massa de nódulos por planta, respectivamente. Considerando todas as cultivares e estirpes de rizóbio, WAF 15 foi a cultivar com melhor desempenho em número e massa nodular. WAF 6 foi a cultivar de pior desempenho, chegando quase ao nível de restrição da nodulação com a estirpe R. etli CIAT 632.

  14. An adolescent case of familial hyperparathyroidism with a germline frameshift mutation of the CDC73 gene

    OpenAIRE

    Takeuchi, Takako; Yoto, Yuko; Tsugawa, Takeshi; Kamasaki, Hotaka; Kondo, Atsushi; OGINO, JIRO; Hasegawa, Tadashi; Yama, Naoya; Anan, Sawa; Uchino, Shinya; Ishikawa, Aki; Sakurai, Akihiro; Tsutsumi, Hiroyuki

    2015-01-01

    Abstract. A 13-yr-old boy who complained of persistent nausea, vomiting and weight loss had hypercalcemia and an elevated intact PTH level. Computed tomography confirmed two tumors in the thyroid gland. The tumors were surgically removed and pathologically confirmed as parathyroid adenoma. Because his maternal aunt and grandmother both had histories of parathyroid tumors, genetic investigation was undertaken for him, and a germline frameshift mutation of the CDC73 gene was identified. CDC73 g...

  15. Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens.

    OpenAIRE

    Funke, G; Lucchini, G M; Pfyffer, G E; Marchiani, M; von Graevenitz, A

    1993-01-01

    Fifteen strains of CDC group 1 coryneform and biochemically similar bacteria were isolated from clinical specimens. Of the 15 strains isolated, 11 were derived from abscesses and purulent lesions, mostly from the upper part of the body, and 3 were grown from blood cultures. Nine strains were associated with mixed anaerobic but no other aerobic flora. Seven strains exhibited the classical biochemical profile of CDC coryneform group 1; however, eight strains were unable to reduce nitrate and we...

  16. Glucose-stimulated Cdc42 Signaling Is Essential for the Second Phase of Insulin Secretion*

    OpenAIRE

    Wang, Zhanxiang; Oh, Eunjin; Thurmond, Debbie C.

    2007-01-01

    The small Rho family GTPases Cdc42 and Rac1 have each been shown to function in insulin exocytosis and are presumed to function in actin remodeling and insulin granule mobilization. However, whether either GTPase is required for the mobilization phase of insulin release (second phase) and are linked in a common signaling pathway has remained unknown. Here we demonstrate that small interfering RNA-mediated depletion of Cdc42 from isolated islets results in the selective loss of second-phase in...

  17. NCAR Johns Hopkins/CDC Climate and Health Summer Institute

    Science.gov (United States)

    Mearns, Linda O.

    2005-01-01

    The interactions between climate and health are rife with complexity and present many conceptual and methodological challenges. Possible effects of climate change on health are considered some of the most sensitive impacts of climate change and are a high priority for policy-makers and the public. As a first step toward improving tlit: quality of research, we developed a Climate and Health Workshop (Institute), geared toward teaching students various aspects of how to conduct integrated climate and health research. At the workshop scientists presented selected case studies of climate and health (e.g., heat mortality, vector-borne diseases), thus demonstrating a subset of key analytical tools and databases most useful to researchers in this field. Key research gaps in this research area were discussed. In this six-day Institute (21-28 July 2004, Boulder, Colorado), health scientists and students benefited from lectures and hands-on tools taught by top NCAR scientists. The attendees learned about health databases and epidemiologic methods from leading health scientists from CDC, Johns Hopkins, and other institutions from around the globe.

  18. CDC Guideline for Prescribing Opioids for Chronic Pain - United States, 2016.

    Science.gov (United States)

    Dowell, Deborah; Haegerich, Tamara M; Chou, Roger

    2016-01-01

    This guideline provides recommendations for primary care clinicians who are prescribing opioids for chronic pain outside of active cancer treatment, palliative care, and end-of-life care. The guideline addresses 1) when to initiate or continue opioids for chronic pain; 2) opioid selection, dosage, duration, follow-up, and discontinuation; and 3) assessing risk and addressing harms of opioid use. CDC developed the guideline using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) framework, and recommendations are made on the basis of a systematic review of the scientific evidence while considering benefits and harms, values and preferences, and resource allocation. CDC obtained input from experts, stakeholders, the public, peer reviewers, and a federally chartered advisory committee. It is important that patients receive appropriate pain treatment with careful consideration of the benefits and risks of treatment options. This guideline is intended to improve communication between clinicians and patients about the risks and benefits of opioid therapy for chronic pain, improve the safety and effectiveness of pain treatment, and reduce the risks associated with long-term opioid therapy, including opioid use disorder, overdose, and death. CDC has provided a checklist for prescribing opioids for chronic pain (http://stacks.cdc.gov/view/cdc/38025) as well as a website (http://www.cdc.gov/drugoverdose/prescribingresources.html) with additional tools to guide clinicians in implementing the recommendations. PMID:26987082

  19. A novel E2 box-GATA element modulates Cdc6 transcription during human cells polyploidization.

    Science.gov (United States)

    Vilaboa, Nuria; Bermejo, Rodrigo; Martinez, Pilar; Bornstein, Rafael; Calés, Carmela

    2004-01-01

    Cdc6 is a key regulator of the strict alternation of S and M phases during the mitotic cell cycle. In mammalian and plant cells that physiologically become polyploid, cdc6 is transcriptionally and post-translationally regulated. We have recently reported that Cdc6 levels are maintained in megakaryoblastic HEL cells, but severely downregulated by ectopic expression of transcriptional repressor Drosophila melanogaster escargot. Here, we show that cdc6 promoter activity is upregulated during megakaryocytic differentiation of HEL endoreplicating cells, and that Escargot interferes with such activation. Transactivation experiments showed that a 1.7 kb region located at 2800 upstream cdc6 transcription initiation site behaved as a potent enhancer in endoreplicating cells only. This activity was mainly dependent on a novel cis-regulatory element composed by an E2 box overlapping a GATA motif. Ectopic Escargot could bind this regulatory element in vitro and endogenous GATA-1 and E2A formed specific complexes in megakaryoblastic cells as well as in primary megakaryocytes. Chromatin Immunoprecipitation analysis revealed that both transcription factors were occupying the E2 box/GATA site in vivo. Altogether, these data suggest that cdc6 expression could be actively maintained during megakaryocytic differentiation through transcriptional mechanisms involving specific cis- and trans-regulatory elements. PMID:15590906

  20. Roles of Aspergillus nidulans Cdc42/Rho GTPase regulators in hyphal morphogenesis and development.

    Science.gov (United States)

    Si, Haoyu; Rittenour, William R; Harris, Steven D

    2016-01-01

    The Rho-related family of GTPases are pivotal regulators of morphogenetic processes in diverse eukaryotic organisms. In the filamentous fungi two related members of this family, Cdc42 and Rac1, perform particularly important roles in the establishment and maintenance of hyphal polarity. The activity of these GTPases is tightly controlled by two sets of regulators: guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Despite the importance of Cdc42 and Rac1 in polarized hyphal growth, the morphogenetic functions of their cognate GEFs and GAPs have not been widely characterized in filamentous fungi outside the Saccharomycotina. Here we present a functional analysis of the Aspergillus nidulans homologs of the yeast GEF Cdc24 and the yeast GAP Rga1. We show that Cdc24 is required for the establishment of hyphal polarity and localizes to hyphal tips. We also show that Rga1 is necessary for the suppression of branching in developing conidiophores. During asexual development Rga1 appears to act primarily via Cdc42 and in doing so serves as a critical determinant of conidiophore architecture. Our results provide new insight into the roles of Cdc42 during development in A nidulans. PMID:26932184

  1. Cellular localization and biochemical analysis of mammalian CDC50A, a glycosylated β-subunit for P4 ATPases.

    Science.gov (United States)

    Folmer, Dineke E; Mok, Kam S; de Wee, Sebastiaan W; Duijst, Suzanne; Hiralall, Johan K; Seppen, Jurgen; Oude Elferink, Ronald P J; Paulusma, Coen C

    2012-03-01

    CDC50 proteins are β-subunits for P4 ATPases, which upon heterodimerization form a functional phospholipid translocation complex. Emerging evidence in mouse models and men links mutations in P4 ATPase genes with human disease. This study analyzed the tissue distribution and cellular localization of CDC50A, the most abundant and ubiquitously expressed CDC50 homologue in the mouse. The authors have raised antibodies that detect mouse and human CDC50A and studied CDC50A localization and glycosylation status in mouse liver cells. CDC50A is a terminal-glycosylated glycoprotein and is expressed in hepatocytes and liver sinusoidal endothelial cells, where it resides in detergent-resistant membranes. In pancreas and stomach, CDC50A localized to secretory vesicles, whereas in the kidney, CDC50A localized to the apical region of proximal convoluted tubules of the cortex. In WIF-B9 cells, CDC50A partially costains with the trans-Golgi network. Data suggest that CDC50A is present as a fully glycosylated protein in vivo, which presumes interaction with distinct P4 ATPases. PMID:22253360

  2. Human TRIB2 Oscillates during the Cell Cycle and Promotes Ubiquitination and Degradation of CDC25C.

    Science.gov (United States)

    Liang, Kai Ling; Paredes, Roberto; Carmody, Ruaidhri; Eyers, Patrick A; Meyer, Stefan; McCarthy, Tommie V; Keeshan, Karen

    2016-01-01

    Tribbles homolog 2 (TRIB2) is a member of the mammalian Tribbles family of serine/threonine pseudokinases (TRIB1-3). Studies of TRIB2 indicate that many of the molecular interactions between the single Drosophila Tribbles (Trbl) protein and interacting partners are evolutionary conserved. In this study, we examined the relationship between TRIB2 and cell division cycle 25 (CDC25) family of dual-specificity protein phosphatases (mammalian homologues of Drosophila String), which are key physiological cell cycle regulators. Using co-immunoprecipitation we demonstrate that TRIB2 interacts with CDC25B and CDC25C selectively. Forced overexpression of TRIB2 caused a marked decrease in total CDC25C protein levels. Following inhibition of the proteasome, CDC25C was stabilized in the nuclear compartment. This implicates TRIB2 as a regulator of nuclear CDC25C turnover. In complementary ubiquitination assays, we show that TRIB2-mediated degradation of CDC25C is associated with lysine-48-linked CDC25C polyubiquitination driven by the TRIB2 kinase-like domain. A cell cycle associated role for TRIB2 is further supported by the cell cycle regulated expression of TRIB2 protein levels. Our findings reveal mitotic CDC25C as a new target of TRIB2 that is degraded via the ubiquitin proteasome system. Inappropriate CDC25C regulation could mechanistically underlie TRIB2 mediated regulation of cellular proliferation in neoplastic cells. PMID:27563873

  3. Cdc6 localizes to S- and G2-phase centrosomes in a cell cycle-dependent manner

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Gwang Su; Kang, Jeeheon; Bang, Sung Woong; Hwang, Deog Su, E-mail: dshwang@snu.ac.kr

    2015-01-16

    Highlights: • Cdc6 protein is a component of the pre-replicative complex required for chromosomal replication initiation. • Cdc6 localized to centrosomes of S and G2 phases in a cell cycle-dependent manner. • The centrosomal localization was governed by centrosomal localization signal sequences of Cdc6. • Deletions or substitution mutations on the centrosomal localization signal interfered with centrosomal localization of the Cdc6 proteins. - Abstract: The Cdc6 protein has been primarily investigated as a component of the pre-replicative complex for the initiation of chromosome replication, which contributes to maintenance of chromosomal integrity. Here, we show that Cdc6 localized to the centrosomes during S and G2 phases of the cell cycle. The centrosomal localization was mediated by Cdc6 amino acid residues 311–366, which are conserved within other Cdc6 homologues and contains a putative nuclear export signal. Deletions or substitutions of the amino acid residues did not allow the proteins to localize to centrosomes. In contrast, DsRed tag fused to the amino acid residues localized to centrosomes. These results indicated that a centrosome localization signal is contained within amino acid residues 311–366. The cell cycle-dependent centrosomal localization of Cdc6 in S and G2 phases suggest a novel function of Cdc6 in centrosomes.

  4. The small GTPase Cdc42 modulates the number of exocytosis-competent dense-core vesicles in PC12 cells

    International Nuclear Information System (INIS)

    Highlights: ► Regulation of exocytosis by Rho GTPase Cdc42. ► Cdc42 increases the number of fusion events from newly recruited vesicles. ► Cdc42 increases the number of exocytosis-competent dense-core vesicles. -- Abstract: Although the small GTPase Rho family Cdc42 has been shown to facilitate exocytosis through increasing the amount of hormones released, the precise mechanisms regulating the quantity of hormones released on exocytosis are not well understood. Here we show by live cell imaging analysis under TIRF microscope and immunocytochemical analysis under confocal microscope that Cdc42 modulated the number of fusion events and the number of dense-core vesicles produced in the cells. Overexpression of a wild-type or constitutively-active form of Cdc42 strongly facilitated high-KCl-induced exocytosis from the newly recruited plasma membrane vesicles in PC12 cells. By contrast, a dominant-negative form of Cdc42 inhibited exocytosis from both the newly recruited and previously docked plasma membrane vesicles. The number of intracellular dense-core vesicles was increased by the overexpression of both a wild-type and constitutively-active form of Cdc42. Consistently, activation of Cdc42 by overexpression of Tuba, a Golgi-associated guanine nucleotide exchange factor for Cdc42 increased the number of intracellular dense-core vesicles, whereas inhibition of Cdc42 by overexpression of the Cdc42/Rac interactive binding domain of neuronal Wiskott–Aldrich syndrome protein decreased the number of them. These findings suggest that Cdc42 facilitates exocytosis by modulating both the number of exocytosis-competent dense-core vesicles and the production of dense-core vesicles in PC12 cells.

  5. MODELI SISTEMA POSLUŽIVANJA

    OpenAIRE

    Varga, Mirko

    1989-01-01

    U članku se raspravlja o modelima sistema posluživanja (MSP). Razrađuju se osnovni elementi MSP-a, navodi se primjer i najznačajnije karakteristike. Ukazuje se također na složenost postavljanja odgovarajuće koncepcije upravljanja transakcijama te navode karakteristike najčešćih načina posluživanja. U trećem dijelu komentiramo najčešća obilježja sistema koje obično tretiramo modelom. U četvrtom dijelu navode se ograničenja modela te iz toga proizašla potreba za proširenjem definicije MSP-a. Na...

  6. Agricultura Apoiada pela Comunidade: poderia a experiência dos agricultores americanos ser útil para os agricultores urbanos brasileiros? Community Supported Agriculture: could the experience of American farmers be useful to Brazilian urban farmers?

    Directory of Open Access Journals (Sweden)

    M Castelo Branco

    2011-03-01

    Full Text Available O projeto "Agricultura Apoiada pela Comunidade" (AAC é uma alternativa de mercado utilizada na Europa e nos EUA. Neste mercado alternativo, os produtores oferecem a sua produção aos consumidores, sob a forma de cestas de alimentos, alguns meses antes de iniciarem a produção de suas culturas. Algum tempo depois, durante alguns meses, os consumidores irão receber os produtos. Atualmente, diversos países têm uma parcela significativa da sua população vivendo na pobreza. Por isso, diversas políticas públicas foram desenhadas nesses países com o objetivo de reduzi-la. O estímulo à agricultura urbana é uma dessas políticas. No entanto, frequentemente, os produtores urbanos pobres têm dificuldades para adquirir os insumos necessários para manter a sua produção. O apoio contínuo do governo local associado ao apoio contínuo da comunidade local poderia ajudar esses produtores a manter o seu sistema de produção. E, para esses agricultores urbanos pobres, o projeto AAC poderia ser um meio alternativo de organização. O objetivo desse trabalho foi obter algumas informações sobre o projeto AAC nos EUA e avaliar se consumidores e produtores urbanos pobres de uma cidade brasileira de baixa renda aceitariam se engajar neste tipo de projeto. Os produtores americanos envolvidos no projeto AAC em geral cultivavam uma área inferior a 2 ha, ofereciam diferentes tipos de hortaliças aos consumidores por pelo menos quarto meses e geralmente usavam práticas de produção orgânica. No entanto, havia dificuldades para a manutenção do projeto, sendo o planejamento da produção e a dificuldade de sua manutenção ao longo do tempo os maiores problemas. Alguns consumidores da cidade brasileira pesquisada se mostraram dispostos a participar deste tipo de projeto principalmente pela possibilidade de receberem alimentos frescos e produzidos organicamente. No entanto, o mesmo não ocorreu com os produtores urbanos pobres da cidade. E a raz

  7. Therapeutic Targeting the Cell Division Cycle 25 (CDC25 Phosphatases in Human Acute Myeloid Leukemia — The Possibility to Target Several Kinases through Inhibition of the Various CDC25 Isoforms

    Directory of Open Access Journals (Sweden)

    Annette K. Brenner

    2014-11-01

    Full Text Available The cell division cycle 25 (CDC25 phosphatases include CDC25A, CDC25B and CDC25C. These three molecules are important regulators of several steps in the cell cycle, including the activation of various cyclin-dependent kinases (CDKs. CDC25s seem to have a role in the development of several human malignancies, including acute myeloid leukemia (AML; and CDC25 inhibition is therefore considered as a possible anticancer strategy. Firstly, upregulation of CDC25A can enhance cell proliferation and the expression seems to be controlled through PI3K-Akt-mTOR signaling, a pathway possibly mediating chemoresistance in human AML. Loss of CDC25A is also important for the cell cycle arrest caused by differentiation induction of malignant hematopoietic cells. Secondly, high CDC25B expression is associated with resistance against the antiproliferative effect of PI3K-Akt-mTOR inhibitors in primary human AML cells, and inhibition of this isoform seems to reduce AML cell line proliferation through effects on NFκB and p300. Finally, CDC25C seems important for the phenotype of AML cells at least for a subset of patients. Many of the identified CDC25 inhibitors show cross-reactivity among the three CDC25 isoforms. Thus, by using such cross-reactive inhibitors it may become possible to inhibit several molecular events in the regulation of cell cycle progression and even cytoplasmic signaling, including activation of several CDKs, through the use of a single drug. Such combined strategies will probably be an advantage in human cancer treatment.

  8. Poslovanje in financiranje zdravstvenega sistema Republike Slovenije

    OpenAIRE

    Gorup, Luka

    2016-01-01

    V nalogi smo podrobneje predstavili zdravstveni sistem Republike Slovenije, ureditev obveznega zdravstvenega zavarovanja in drugih virov, s katerimi financiramo zdravstveni sistem. Preučili smo poslovanje sistema, plačevanje zdravstvenih izvajalcev in zaposlenih ter predstavili partnerje pri upravljanju sistema. Natačneje smo predstavili porazdelitev in ureditev različnih ravni zdravstvenega sistema, razporeditev in organiziranost človeških, finančnih, materialnih in drugih virov. Sis...

  9. Thyroid bud morphogenesis requires CDC42- and SHROOM3-dependent apical constriction

    Science.gov (United States)

    Loebel, David A. F.; Plageman, Timothy F.; Tang, Theresa L.; Jones, Vanessa J.; Muccioli, Maria; Tam, Patrick P. L.

    2016-01-01

    ABSTRACT Early development of the gut endoderm and its subsequent remodeling for the formation of organ buds are accompanied by changes to epithelial cell shape and polarity. Members of the Rho-related family of small GTPases and their interacting proteins play multiple roles in regulating epithelial morphogenesis. In this study we examined the role of Cdc42 in foregut development and organ bud formation. Ablation of Cdc42 in post-gastrulation mouse embryos resulted in a loss of apical-basal cell polarity and columnar epithelial morphology in the ventral pharyngeal endoderm, in conjunction with a loss of apical localization of the known CDC42 effector protein PARD6B. Cell viability but not proliferation in the foregut endoderm was impaired. Outgrowth of the liver, lung and thyroid buds was severely curtailed in Cdc42-deficient embryos. In particular, the thyroid bud epithelium did not display the apical constriction that normally occurs concurrently with the outgrowth of the bud into the underlying mesenchyme. SHROOM3, a protein that interacts with Rho GTPases and promotes apical constriction, was strongly expressed in the thyroid bud and its sub-cellular localization was disrupted in Cdc42-deficient embryos. In Shroom3 gene trap mutant embryos, the thyroid bud epithelium showed no apical constriction, while the bud continued to grow and protruded into the foregut lumen. Our findings indicate that Cdc42 is required for epithelial polarity and organization in the endoderm and for apical constriction in the thyroid bud. It is possible that the function of CDC42 is partly mediated by SHROOM3. PMID:26772200

  10. Sistema de transparencia en México

    OpenAIRE

    Alejandro D. Pérez Corzo

    2013-01-01

    Actualmente, hablar del «sistema de transparencia» en México, es referirse a un término que no ha sido generalmente delimitido o reconocido. Se habla de sistema constitucional, sistema electoral como conceptos cuyos elementos y funcionamiento están aceptablemente bien definidos. Es así que se vuelve imprescindible, por todos los cambios, reformas e iniciativas de ley, en el ámbito energético, en el sistema educativo nacional, en el poder judicial, económico asir el concepto. En los dos último...

  11. La reforma del sistema electoral chileno

    OpenAIRE

    Mariela Rubano Lapasta

    2007-01-01

    La modificación del sistema electoral público chileno se ha transformado nuevamente en tema de debate público, después de la Reforma Constitucional de 2005. El Gobierno ha propuesto reformar el sistema electoral, sustituirlo por otro que permita a las minorías tener representación en el Congreso. Se abordan brevemente los sistemas electorales en el derecho comparado, en especial el caso chileno, evaluando las ventajas y desventajas del sistema electoral nacional. Se propone ...

  12. Gerencia de sistemas conductuales y cambio organizacional

    OpenAIRE

    Guillermo E. Yáber; María E. Malott; Elizabeth Valarino

    1998-01-01

    La gerencia de sistemas conductuales es una tecnología basada en un enfoque científico para el análisis del comportamiento en sistemas humanos. Esta tecnología ayuda a las organizaciones a definir y lograr sus metas. Consiste en el análisis, especificación, diseño, implementación, evaluación y reciclaje de sistemas de motivación humana que optimizan el desempeño y desarrollo a nivel del sistema total, departamental e individual de la organización. Utiliza tres modelos: El di...

  13. Hepatocyte-specific deletion of Cdc42 results in delayed liver regeneration after partial hepatectomy in mice

    DEFF Research Database (Denmark)

    Yuan, Haixin; Zhang, Hong; Wu, Xunwei;

    2009-01-01

    Cdc42, a member of the Rho guanosine triphosphatase (GTPase) family, plays important roles in the regulation of the cytoskeleton, cell proliferation, cell polarity, and cellular transport, but little is known about its specific function in mammalian liver. We investigated the function of Cdc42 in...... regeneration, which may result from impaired cytoskeletal organization and intracellular trafficking in hepatocytes. Conclusion: Our results revealed important roles of Cdc42 in the regulation of proliferative signaling during liver regeneration....

  14. El envío de indios americanos a la Península Ibérica: aspectos legales (1492-1542

    Directory of Open Access Journals (Sweden)

    Esteban MIRA CABALLOS

    2009-12-01

    Full Text Available RESUMEN: En este presente artículo ofrecemos por primera vez una sistematización sobre la legislación existente en torno a la trata de indios americanos con destino a los mercados esclavistas de la Península Ibérica. Se trata sin duda de un trabajo sumamente novedoso que aporta nueva luz a un aspecto de la Historia Moderna de España que ha pasado casi desapercibido hasta la fecha para la historiografía más reciente. La conclusión principal que se puede extraer de este trabajo es la negativa de la Corona -salvo en los dubitativos momentos iniciales- a que se trajesen indios esclavos a la Península. No obstante, esta legislación prohibitiva sólo sirvió para que los envíos se ralentizaran sin erradicar totalmente una trata que siempre encontró las formas de continuar de una manera más o menos fraudulenta.ABSTRACT: For the first time the present article offers us a systematization about the existing laws concerning the American indians trade with the Iberian Peninsula slaves markets. Undoubtledly, it consists of an extremely original work which casts light on one aspect of the Spanish early modern history that has gone unnoticed to the recent historiography. The main conclusion to be drawn from this work is the Crown's denial for the Indian slaves to be brought, except for the doubts arisen at the beginning. This prohibitive laws only served to slow down the slave shipment without eradicate this practice that always founds to continue new forms, more or less fraudulent.

  15. Depois das reformas: os regimes de proteção social latino-americanos na década de 2000

    Directory of Open Access Journals (Sweden)

    Rodrigo Cantu

    2015-12-01

    Full Text Available Resumo O artigo caracteriza os regimes de política social na América Latina durante a década de 2000, um contexto que vem sendo designado por uma penetração menos acentuada de políticas neoliberais. Usamos o método de Análise de Componentes Principais (ACP para analisar dados sobre o papel do Estado, do mercado e das famílias na proteção contra riscos sociais, recolhidos em fontes como CEPAL, FMI, Banco Mundial, OMS, entre outras. Comparando os dados para as décadas de 1990 e de 2000, identificamos certa estabilização das reformas neoliberais. Comparando ainda os resultados da ACP com a literatura, sobretudo com a literatura que se ocupa das décadas de 1980 e 1990, argumentamos que, mesmo atenuadas, as reformas deixaram o cenário da política social mais complexo na América Latina na década de 2000. Uma primeira dimensão, já amplamente discutida na literatura, foi encontrada na ACP, definida pelo grau em que o Estado inclui a população em suas políticas sociais. Ademais, uma segunda dimensão relevante foi revelada pela análise, captando em que profundidade o mercado se instalou como princípio da política social. Discutimos a hipótese segundo a qual essa dimensão se tornou expressiva após as reformas das décadas de 1980 e 1990. Junto com o argumento de que a literatura precisa ser atualizada com base nesse quadro mais complexo, destacamos a diversidade dos regimes de política social latino-americanos tanto internamente quanto em comparação com outras regiões do mundo.

  16. Las maestras y el ensayo como escritura cívica femenina en Repertorio Americano / Women Teachers, Essays and Civic Writing in “Repertorio Americano”

    Directory of Open Access Journals (Sweden)

    Marybel Soto Ramírez

    2011-11-01

    Full Text Available Recibido 17 de mayo de 2011 • Aceptado 26 de agosto de 2011 • Corregido 9 de octubre de 2011 Resumen. La escritura femenina, como todas las formas de producción simbólica desde las mujeres, ha existido y se ha desarrollado a pesar del mandato patriarcal de obedecer y callar que pesa sobre el género femenino. No es objetivo en esta reflexión, ahondar en las acciones y las obras de las mujeres transgresoras quienes, a lo largo de la historia y a pesar de, han ejercido esa forma de hacerse presente y afirmarse ante los otros que es la escritura. Mi propósito es identificar a las mujeres, quienes desde el oficio de maestras ejercitaron su escritura, combinaron su rol docente con el de escritoras y en la elaboración de sus discursos cívicos cuestionaron, precisamente, las concepciones de patriotismo y sentimiento cívico dentro del mismo proceso de construcción de una ciudadanía de la cual ellas no disfrutaban. Abstract. Women writing, as any symbolic form of production, has existed and developed despite of the patriarchal imperative commanding women to obey and to remain silent. It is not the objective of this essay to deepen on the deeds and doings of those women who, throughout history and despite patriarchal mandates, have exerted their writing as a transgression as a way to show and to affirm themselves before others. Moreover, my purpose is to identify women in Repertorio Americano (1919-1958 who as teachers and writers elaborated civic discourses and questioned the very meaning of patriotism and civic feelings in the constructions of a citizenship they were not fully and formally entitled to.

  17. The F-box protein Cdc4/Fbxw7 is a novel regulator of neural crest development in Xenopus laevis

    Directory of Open Access Journals (Sweden)

    Hartley Rebecca S

    2010-01-01

    Full Text Available Abstract Background The neural crest is a unique population of cells that arise in the vertebrate ectoderm at the neural plate border after which they migrate extensively throughout the embryo, giving rise to a wide range of derivatives. A number of proteins involved in neural crest development have dynamic expression patterns, and it is becoming clear that ubiquitin-mediated protein degradation is partly responsible for this. Results Here we demonstrate a novel role for the F-box protein Cdc4/Fbxw7 in neural crest development. Two isoforms of Xenopus laevis Cdc4 were identified, and designated xCdc4α and xCdc4β. These are highly conserved with vertebrate Cdc4 orthologs, and the Xenopus proteins are functionally equivalent in terms of their ability to degrade Cyclin E, an established vertebrate Cdc4 target. Blocking xCdc4 function specifically inhibited neural crest development at an early stage, prior to expression of c-Myc, Snail2 and Snail. Conclusions We demonstrate that Cdc4, an ubiquitin E3 ligase subunit previously identified as targeting primarily cell cycle regulators for proteolysis, has additional roles in control of formation of the neural crest. Hence, we identify Cdc4 as a protein with separable but complementary functions in control of cell proliferation and differentiation.

  18. Anti-CDC25B autoantibody predicts poor prognosis in patients with advanced esophageal squamous cell carcinoma

    OpenAIRE

    Dong Jun; Zeng Bo-hang; Xu Li-hua; Wang Jun-ye; Li Man-Zhi; Zeng Mu-sheng; Liu Wan-li

    2010-01-01

    Abstract Background The oncogene CDC25B phosphatase plays an important role in cancer cell growth. We have recently reported that patients with esophageal squamous cell carcinoma (ESCC) have significantly higher serum levels of CDC25B autoantibodies (CDC25B-Abs) than both healthy individuals and patients with other types of cancer; however, the potential diagnostic or prognostic significance of CDC25B-Abs is not clear. The aim of this study is to evaluate the clinical significance of serum CD...

  19. The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse

    DEFF Research Database (Denmark)

    Vasyutina, Elena; Martarelli, Benedetta; Brakebusch, Cord; Wende, Hagen; Birchmeier, Carmen

    2009-01-01

    Rac1 and Cdc42 are small G-proteins that regulate actin dynamics and affect plasma membrane protrusion and vesicle traffic. We used conditional mutagenesis in mice to demonstrate that Rac1 and Cdc42 are essential for myoblast fusion in vivo and in vitro. The deficit in fusion of Rac1 or Cdc42...... genetic analysis demonstrates thus that the function of Rac in myoblast fusion is evolutionarily conserved from insects to mammals and that Cdc42, a molecule hitherto not implicated in myoblast fusion, is essential for the fusion of murine myoblasts....

  20. Drug design with Cdc7 kinase: a potential novel cancer therapy target

    Directory of Open Access Journals (Sweden)

    Masaaki Sawa

    2008-11-01

    Full Text Available Masaaki Sawa1, Hisao Masai21Carna Biosciences, Inc., Kobe, Japan; 2Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanAbstract: Identification of novel molecular targets is critical in development of new and efficient cancer therapies. Kinases are one of the most common drug targets with a potential for cancer therapy. Cell cycle progression is regulated by a number of kinases, some of which are being developed to treat cancer. Cdc7 is a serine-threonine kinase originally discovered in budding yeast, which has been shown to be necessary to initiate the S phase. Inhibition of Cdc7 in cancer cells retards the progression of the S phase, accumulates DNA damage, and induces p53-independent cell death, but the same treatment in normal cells does not significantly affect viability. Low-molecular-weight compounds that inhibit Cdc7 kinase with an IC50 of less than 10 nM have been identified, and shown to be effective in the inhibition of tumor growth in animal models. Thus Cdc7 kinase can be recognized as a novel molecular target for cancer therapy.Keywords: Cdc7 kinase, cell cycle, replication fork, genome stability, DNA damages, ATP-binding pocket, kinase inhibitor

  1. Temperature dependent expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    p34cdc2 and Cyclin Bi are key components of cell cycle controlling machine and are believed to play a fundamental role in gametogenesis. It is also well known that, in scrotal mammals, spermatogenesis depends greatly on the maintenance of comparatively low temperature in the scrotum. To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that the abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes, but it blocked the translation of them. Due to the deficiency of p34cdc2 and Cyclin B1, the spermatogonia and pachytene/diplotene primary spermatocytes were unable to form MPF, hence, they couldn't undergo karyokinesis. The development of primary spermato cytes was arrested at the G2 to M phase transition. We also found that testosterone could regulate the Cyclin B1 expression in spermatogenic cells. Muscular injection of testosterone could recover spermatogenesis in the unilateral scrotal testis which was influenced by the contralateral cryptorchid testis, but it could not salvage the spermatogenesis block in the cryptorchid testis.

  2. Sistema de apoyo al aprendizaje diagnóstico: de endodiag a un sistema multiagente

    OpenAIRE

    Casali, Ana; Corti, Rosa Marina; D'Agostino, Estela; Biga, Adrián; Siragusa, Martha; Aciar, Silvana

    2005-01-01

    En este trabajo se describe el desarrollo y evolución, a través de distintos prototipos, de un sistema de apoyo a la enseñanza diagnóstica en Endodoncia. Este sistema tiene como objetivo reforzar el conocimiento que adquieren los estudiantes a través de la práctica clínica. Los dos primeros prototipos de este sistema se han desarrollado para ser ejecutados por los alumnos en una sola Terminal. El primero de estos sistemas es EndoDiag, un sistema experto que acompaña al alumno en el diagnóstic...

  3. Distrito federal electoral No.34 del estado de California: un estudio de caso sobre la inclusión y representación de ciudadanos americanos de origen mexicano en el sistema político estadounidense

    OpenAIRE

    Cebreros Félix, Erika Eloisa

    2004-01-01

    A partir del censo poblacional de Estados Unidos de América de 2000, se determinó que los hispanos conformarían el grupo minoritario más grande del país, por lo que se les empezó a visualizar en términos políticos y específicamente electorales. Sin embargo, la historia y evolución de la inclusión y representación de los diferentes subgrupos que conforman dicha minoría ha sido poco desarrollada. El concepto de representación es uno de los aspectos fundamentales en el estud...

  4. Analysis of the influence of the expansion of the South American electric system in emissions of greenhouse gases; Analise da influencia da expansao do sistema eletrico Sul-Americano nas emissoes de gases de efeito estufa

    Energy Technology Data Exchange (ETDEWEB)

    Castagna, Annemarlen Gehrke [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Blesl, Markus [Institute of Economics and the Rational Use of Energie (IER), Stuttgart (Germany)

    2010-07-01

    South America combines economic and population growth with a consequent rapid increase in electricity demand. This can only be covered by building new power plants, use of the remaining renewable potential and expansion of transmission lines. The expansion of supply in all regions, with reliable generation and transmission systems is the greatest challenge for the continent in order to reduce social differences and not to curb economic development. To support the energy planning the application of system models represents useful method. This paper intends to analyze the expansion effect of power plant parks in regard of greenhouse gases emissions using a regionalized model system 'TIMES (The Integrated Markal - EFOM System)'. The model includes 10 South American countries (Argentina, Brazil, Bolivia, Chile, Colombia, Ecuador, Paraguay, Peru, Uruguay and Venezuela) with their respective power parks and transmission lines, demand divided in sectors, potential use of renewable energy sources, gas pipelines and possibilities of new interconnections within and between countries. As results are obtained the future installed capacity and generation according the energy use, greenhouse gases emissions, as well as the investments needed to expand the electric system in different scenarios. (author)

  5. Argentina en el sistema internacional

    OpenAIRE

    Tini, María Natalia

    2002-01-01

    El presente trabajo apunta a analizar cuál es el lugar "real" que ocupa la Argentina en el Sistema Internacional, en contraste, con el lugar "ideal" en que lo ha ido posicionando nuestra dirigencia en los últimos 10 años. Para ello, es esencial realizar un breve repaso de la política exterior llevada a cabo por las administraciones Menem y De La Rúa, como sustento empírico de dicho análisis. La hipótesis plantea que para proyectar una adecuada política exterior es necesario formular un ...

  6. Lituanistikos informacinė sistema

    OpenAIRE

    Kučiukas, Tomas

    2010-01-01

    Šio darbo tyrimo sritis – programinės įrangos projektavimo metodai, leidžiantys išplėsti egzistuojančias sistemas, ir jų taikymas bibliotekų informacinei sistemai. Tyrimo objektas – mokslo publikacijų, atitinkančių lituanistikos sampratą, bibliografinės duomenų bazės projektavimas ir kūrimas. Darbo tikslas – pagerinti lituanistikos sampratą atitinkančių mokslo publikacijų vertinimo bei sklaidos galimybes, automatizuoti šių publikacijų pateikimo, ekspertavimo bei sklaidos procesus....

  7. Mobiliario urbano: sistema de bancos

    OpenAIRE

    Vallati, Emiliano

    2012-01-01

    Para desarrollar el presente proyecto se partió de considerar al mobiliario urbano como todos los elementos que forman parte del paisaje de la ciudad y que tienen la finalidad de ser útiles para el ciudadano. En este caso, se diseñó un sistema de bancos que interactúan entre sí y que permite incorporar más piezas según el uso, las funciones o las necesidades que se quieran satisfacer. El objetivo fue resolver el problema de sentarse. Para ello, se partió de la acción de sentarse, y se la p...

  8. Neurocitoma no sistema nervoso central

    Directory of Open Access Journals (Sweden)

    José Torquato Severo

    1973-03-01

    Full Text Available É relatado o caso de uma paciente com 6 anos de idade, hospitalizada com síndrome de hipertensão intracraniana. Após o exame neuro-radiológico que evidenciou processo expansivo frontal direito, a paciente foi submetida à cirurgia, durante a qual ocorreu o óbito. O exame histo-patológico do material retirado durante o ato operatório, permitiu o diagnóstico de neurocitoma, tumor raro no sistema nervoso central.

  9. Sistemas distribuidos de tiempo real

    OpenAIRE

    Ramón, Hugo Dionisio; Romero, Fernando; Pesado, Patricia Mabel; Bertone, Rodolfo Alfredo; Boracchia, Marcos; Thomas, Pablo Javier; Esponda, Silvia

    2004-01-01

    El objetivo de esta línea de investigación es el estudio y desarrollo de sistemas de software que poseen restricciones temporales. Resulta de interés el estudio de diferentes metodologías para la especificación y desarrollo de aplicaciones en tiempo real, así como las plataformas donde dichas aplicaciones pueden ejecutarse. Se trabaja experimentalmente con distintos tipos de hardware y software entre los que pueden mencionarse placas A/D, mini-robots, máquinas multimicroprocesadores y dife...

  10. Sistemas distribuidos de tiempo real

    OpenAIRE

    Ramón, Hugo Dionisio; Romero, Fernando; González, Alejandro Héctor; Novarini, Pablo; Estrebou, César Armando

    2005-01-01

    El objetivo de esta línea de investigación es el estudio y desarrollo de sistemas de software que poseen restricciones temporales. Resulta de interés el estudio de diferentes metodologías para la especificación y desarrollo de aplicaciones en tiempo real, así como las plataformas donde dichas aplicaciones pueden ejecutarse. Se trabaja experimentalmente con distintos tipos de hardware y software entre los que pueden mencionarse placas A/D, mini-robots, plataformas de hardware (PC, PIC, etc....

  11. Isolation and Characterization of New Alleles of the Cyclin-Dependent Kinase Gene CDC28 with Cyclin-Specific Functional and Biochemical Defects

    OpenAIRE

    Levine, Kristi; Oehlen, L. J. W. M.; Cross, Frederick R.

    1998-01-01

    The G1 cyclin Cln2 negatively regulates the mating-factor pathway. In a genetic screen to identify factors required for this regulation, we identified an allele of CDC28 (cdc28-csr1) that blocked this function of Cln2. Cln2 immunoprecipitated from cdc28-csr1 cells was completely defective in histone H1 kinase activity, due to defects in Cdc28 binding and activation by Cln2. In contrast, Clb2-associated H1 kinase and Cdc28 binding was normal in immunoprecipitates from these cells. cdc28-csr1 w...

  12. Sistema de videovigilancia utilizando recursos de LAN

    OpenAIRE

    Fernández García, David

    2007-01-01

    El objetivo principal de este proyecto es realizar un sistema de información que sea capaz de efectuar video vigilancia. Este sistema de información está formado por un servidor y un número, en principio, limitado de clientes

  13. 超表达Cdc20基因不影响牛卵母细胞第一极体排出%Over-expression of Cdc20 Gene Has No Effect on Bovine Oocytes First Polar Body Extrusion

    Institute of Scientific and Technical Information of China (English)

    杨文琳; 安鹏; 李伟; 赵贵民; 史芸安; 雷安民

    2012-01-01

    As one of the co-activator of anaphase-promoting complex ( APC) , cell division cycle 20 (CDC20) protein also functions as the target of the spindle assembly checkpoint ( SAC), which is essential for the cell cycle regulation. To investigate the function of Cdc20 during the first polar body extrusion ( PBE I) , Cdc20 CDS was cloned and eukaryotic expression vector pCdc20-Venus was constructed. Using the linear pCdc20-Venus as template, the capped Cdc20-Venus mRNA was synthesized via T7 Mmessage Mmachine Kit ( Ambion). Cdc20 over-expression was performed by microinjection of Cdc20-Venus mRNA into the cytoplasm of bovine oocytes. The results showed that Venus tagged Cdc20 dispersed around the nucleus in HeLa cells. In bovine oocytes, the fluorescence appeared in the whole cytoplasm. However, the PBE I rate in over-expressed group (48. 9% ) is not significant, compared to Venus mRNA injection group (50.9%) and non-injection group (51.1%). Our study demonstrated that the over-expression of Cdc20 in bovine oocytes does not affect the PBE I rate ( P > 0.05).%CDC20(cell division cycle 20)是后期促进复合物(anaphase-promoting complex,APC)的共激活剂之一,也是纺锤体组装检查点(spindle assembly checkpoint,SAC)的靶点,在细胞周期调控中扮演重要角色.为探讨Cdc20在第一极体排出(first polar body extrusion,PBE I)中的作用,Cdc20基因被成功克隆并构建了真核表达载体pCdc20-Venus,随后用T7 Mmessage Mmachine Kit(Ambion)以线性化pCdc20-Venus为模板体外转录(in vitro transcription)获得带帽的Cdc20-Venus mRNA,将Ccdc20-Venus mRNA显微注射到体外培养的牛卵母细胞胞质中进行超量表达.结果表明,真核表达载体pCdc20-Venus转染HeLa细胞后能够正常表达,绿色荧光在细胞核周围呈弥散状分布;将Cdc20-Venus mRNA注射到牛卵母细胞胞质后,胞质内有绿色荧光出现.Cdc20-Venus mRNA注射组卵母细胞的PBE I率(48.9%)与Venus mRNA注射组卵母细胞的PBE I率(50

  14. Representações da Guerra Fria no discurso jornalístico norte-americano de Drew Pearson (1950-1960) =Representations of the Cold War in the north american journalistic discourse of Drew Pearson (1950-1960)

    OpenAIRE

    Portela, Leonice

    2014-01-01

    O presente artigo pretende realizar uma análise nas páginas da revista O Cruzeiro no período de 1950 a 1960, na coluna Carrossel do Mundo escrita pelo jornalista norte-americano Drew Person. Nesta pesquisa evidenciaremos o cruzamento da história com a imprensa e as representações que esta fazia do contexto dos anos de 1950 a 1960. Além de utilizarmos a revistas O Cruzeiro como fonte de pesquisa, está também será nosso objeto de estudo na medida em que se pretende analisar a revista nos seus i...

  15. Una actividad de educación ambiental basada en observaciones del cangrejo de río americano (Procambarus clarkii) en el Barranco del Cercado (Tenerife, Islas Canarias)

    OpenAIRE

    Mingorance Rodríguez, María del Carmen; Gómez Gómez, Juan Imeldo

    2002-01-01

    El Procambarus clarkii (cangrejo de río americano o cangrejo rojo de las marismas) es un ejemplo de la introducción de una especie en un ecosistema. En este trabajo se utiliza una población de esta especie en la isla de Tenerife para un conjunto de actividades educativas en un Instituto de Formación Profesional. Estas actividades incluyen muestreos y capturas de los cangrejos en el campo, la toma de sus medidas iniciales y su mantenimiento en el Instituto.

  16. JUAN M. LOPE BLANCH. El habla de Diego de Ordaz. Contribución a la historia del español americano. México, UNAM. 1985. 233 p.

    Directory of Open Access Journals (Sweden)

    J. L. R.

    1988-06-01

    Full Text Available Este libro reúne los trabajos publicados por Lope Blanch en revistas especializadas y homenajes académicos acerca del idiolecto de este importante americano nacido en tierras leonesas hacia 1480, colonizador de las Antillas, expedicionario a Tierra Firme y a Cuba, conquistador de México y explorador del Orinoco. La base documental está constituida por siete cartas autógrafasque L.B. publica al final del libro en una cuidadosa transcripción que corrige defectos de una edición anterior.

  17. Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Zoltán Kevei

    Full Text Available BACKGROUND: The CDC20 and Cdh1/CCS52 proteins are substrate determinants and activators of the Anaphase Promoting Complex/Cyclosome (APC/C E3 ubiquitin ligase and as such they control the mitotic cell cycle by targeting the degradation of various cell cycle regulators. In yeasts and animals the main CDC20 function is the destruction of securin and mitotic cyclins. Plants have multiple CDC20 gene copies whose functions have not been explored yet. In Arabidopsis thaliana there are five CDC20 isoforms and here we aimed at defining their contribution to cell cycle regulation, substrate selectivity and plant development. METHODOLOGY/PRINCIPAL FINDINGS: Studying the gene structure and phylogeny of plant CDC20s, the expression of the five AtCDC20 gene copies and their interactions with the APC/C subunit APC10, the CCS52 proteins, components of the mitotic checkpoint complex (MCC and mitotic cyclin substrates, conserved CDC20 functions could be assigned for AtCDC20.1 and AtCDC20.2. The other three intron-less genes were silent and specific for Arabidopsis. We show that AtCDC20.1 and AtCDC20.2 are components of the MCC and interact with mitotic cyclins with unexpected specificity. AtCDC20.1 and AtCDC20.2 are expressed in meristems, organ primordia and AtCDC20.1 also in pollen grains and developing seeds. Knocking down both genes simultaneously by RNAi resulted in severe delay in plant development and male sterility. In these lines, the meristem size was reduced while the cell size and ploidy levels were unaffected indicating that the lower cell number and likely slowdown of the cell cycle are the cause of reduced plant growth. CONCLUSIONS/SIGNIFICANCE: The intron-containing CDC20 gene copies provide conserved and redundant functions for cell cycle progression in plants and are required for meristem maintenance, plant growth and male gametophyte formation. The Arabidopsis-specific intron-less genes are possibly "retrogenes" and have hitherto undefined

  18. Dynamics of Cdc42 network embodies a Turing-type mechanism of yeast cell polarity.

    Science.gov (United States)

    Goryachev, Andrew B; Pokhilko, Alexandra V

    2008-04-30

    Complex biochemical networks can be understood by identifying their principal regulatory motifs and mode of action. We model the early phase of budding yeast cellular polarization and show that the biochemical processes in the presumptive bud site comprise a Turing-type mechanism. The roles of the prototypical activator and substrate are played by GTPase Cdc42 in its active and inactive states, respectively. We demonstrate that the nucleotide cycling of Cdc42 converts cellular energy into a stable cluster of activated Cdc42. This energy drives a continuous membrane-cytoplasmic exchange of the cluster components to counteract diffusive spread of the cluster. This exchange explains why only one bud forms per cell cycle, because the winner-takes-all competition of candidate sites inevitably selects a single site. PMID:18381072

  19. Podocyte-specific loss of cdc42 leads to congenital nephropathy

    DEFF Research Database (Denmark)

    Scott, Rizaldy P; Hawley, Steve P; Ruston, Julie; Du, Jianmei; Brakebusch, Cord; Jones, Nina; Pawson, Tony

    2012-01-01

    Rho family GTPases are molecular switches best known for their pivotal role in dynamic regulation of the actin cytoskeleton. The prototypic members of this family are Cdc42, Rac1, and RhoA; these GTPases contribute to the breakdown of glomerular filtration and the resultant proteinuria, but their...... functions in normal podocyte physiology remain poorly understood. Here, mice lacking Cdc42 in podocytes developed congenital nephropathy and died as a result of renal failure within 2 weeks after birth. In contrast, mice lacking Rac1 or RhoA in podocytes were overtly normal and lived to adulthood. Kidneys...... from Cdc42-mutant mice exhibited protein-filled microcysts with hallmarks of collapsing glomerulopathy, as well as extensive effacement of podocyte foot processes with abnormal junctional complexes. Furthermore, we observed aberrant expression of several podocyte markers and cell polarity proteins in...

  20. Riesgos para el sistema mundial

    Directory of Open Access Journals (Sweden)

    José María Tortosa

    2012-02-01

    Full Text Available Partiendo de varios diagnósticos producidos recientemente sobre los riesgos del sistema mundial, se hace una propia lista de los que parecen ser los diez más importantes, a saber, la cuestión del petróleo, el auge de la China, el desplome de los Estados Unidos, la desconexión de América Latina, la crisis de Naciones Unidas, los nuevos terrorismos, la amenaza nuclear, pandemias, cambio climático y polarización social. Como existe una conexión y retroalimentación entre muchos de ellos, se concluye indicando la posibilidad deque estemos entrando en una etapa particularmente caótica en el sistema mundial.Starting from some recent diagnosis about the risks of the world system, a new list is made with the possible more important ones, namely, tipping point in oil, rise of China, breakdown of the United States, new terrorisms, nuclear threat, pandemics, climatic change and social polarization. As far as a connexion and feedback between these elements can be found, the conclusion points to the possibility of a new era especially chaotic in the world system.

  1. Experience in programming Assembly language of CDC CYBER 170/750 computer

    International Nuclear Information System (INIS)

    Aiming to optimize processing time of BCG computer code in the CDC CYBER 170/750 computer, the FORTRAN-V language of INTERP subroutine was converted to Assembly language. The BCG code was developed for solving neutron transport equation by iterative method, and the INTERP subroutine is innermost loop of the code carrying out 5 interpolation types. The central processor unit Assembly language of the CDC CYBER 170/750 computer and its application in implementing the interpolation subroutine of BCG code are described. (M.C.K.)

  2. An adolescent case of familial hyperparathyroidism with a germline frameshift mutation of the CDC73 gene.

    Science.gov (United States)

    Takeuchi, Takako; Yoto, Yuko; Tsugawa, Takeshi; Kamasaki, Hotaka; Kondo, Atsushi; Ogino, Jiro; Hasegawa, Tadashi; Yama, Naoya; Anan, Sawa; Uchino, Shinya; Ishikawa, Aki; Sakurai, Akihiro; Tsutsumi, Hiroyuki

    2015-10-01

    A 13-yr-old boy who complained of persistent nausea, vomiting and weight loss had hypercalcemia and an elevated intact PTH level. Computed tomography confirmed two tumors in the thyroid gland. The tumors were surgically removed and pathologically confirmed as parathyroid adenoma. Because his maternal aunt and grandmother both had histories of parathyroid tumors, genetic investigation was undertaken for him, and a germline frameshift mutation of the CDC73 gene was identified. CDC73 gene analysis should be done on individuals who are at risk of familial hyperparathyroidism, including those who are asymptomatic, and they should be followed for potential primary hyperparathyroidism and associated disorders including resultant parathyroid carcinoma. PMID:26568659

  3. High selectivity of TiC-CDC for CO2/N2 separation

    OpenAIRE

    Silvestre Albero, Ana; Rico Francés, Soledad; Rodríguez Reinoso, Francisco; Kern, Andreas M.; Klumpp, Michael; Etzold, Bastian J. M.; Silvestre Albero, Joaquín

    2013-01-01

    A series of carbide-derived carbons (CDC) have been prepared starting from TiC and using different chlorine treatment temperatures (500–1200 °C). Contrary to N2 adsorption measurements at −196 °C, CO2 adsorption measurements at room temperature and high pressure (up to 1 MPa) together with immersion calorimetry measurements into dichloromethane suggest that the synthesized CDC exhibit a similar porous structure, in terms of narrow pore volume, independently of the temperature of the reactive ...

  4. Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells

    DEFF Research Database (Denmark)

    Czuchra, Aleksandra; Wu, Xunwei; Meyer, Hannelore;

    2005-01-01

    of Cdc42 did not affect filopodium or lamellipodium formation and had no significant influence on the speed of directed migration nor on mitosis. Cdc42-deficient cells displayed a more elongated cell shape and had a reduced area. Furthermore, directionality during migration and reorientation of the...

  5. 77 FR 66469 - CDC/HRSA Advisory Committee on HIV, Viral Hepatitis and STD Prevention and Treatment

    Science.gov (United States)

    2012-11-05

    ... Client Level Data Update; (3) Viral Hepatitis Action Plan and Implementation Update; (4) Update on... Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, CDC, 1600 Clifton Road NE., Mailstop E-07... HUMAN SERVICES Centers for Disease Control and Prevention CDC/HRSA Advisory Committee on HIV,...

  6. 76 FR 29755 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2011-05-23

    ...), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES) In accordance with section 10(a.... Purpose: The ES will provide counsel to the ACD, CDC, regarding a broad range of public health ethics... meeting; discussion of next steps on addressing potential public health ethical issues associated...

  7. 75 FR 57044 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2010-09-17

    ...), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES) In accordance with section 10(a..., CDC, regarding a broad range of public health ethics questions and issues arising from programs..., local and territorial health departments address ethical issues in the practice of public health;...

  8. 77 FR 2549 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2012-01-18

    ...), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES) In accordance with section 10(a... provide counsel to the ACD, CDC, regarding a broad range of public health ethics questions and issues... health departments in their efforts to address public health ethics challenges, approaches for...

  9. Cold-sensitive mutants of p34cdc2 that suppress a mitotic catastrophe phenotype in fission yeast.

    Science.gov (United States)

    Ayscough, K; Hayles, J; MacNeill, S A; Nurse, P

    1992-04-01

    The p34cdc2 protein kinase plays a central role in the regulation of the eukaryotic cell cycle, being required both in late G1 for the commitment to S-phase and in late G2 for the initiation of mitosis. p34cdc2 also determines the precise timing of entry into mitosis in fission yeast, where a number of gene products that regulate p34cdc2 activity have been identified and characterised. To investigate further the mitotic role of p34cdc2 in this organism we have isolated new cold-sensitive p34cdc2 mutants. These are defective only in their G2 function and are extragenic suppressors of the lethal premature entry into mitosis brought about by mutating the mitotic inhibitor p107wee1 and overproducing the mitotic activator p80cdc25. One of the mutant proteins p34cdc2-E8 is only functional in the absence of p107wee1, and all the mutant strains have reduced histone H1 kinase activity in vitro. Each mutant allele has been cloned and sequenced, and the lesions responsible for the cold-sensitive phenotypes identified. All the mutations were found to map to regions that are conserved between the fission yeast p34cdc2 and functional homologues from higher eukaryotes. PMID:1316996

  10. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry

    DEFF Research Database (Denmark)

    Hein, Jamin B; Nilsson, Jakob

    2016-01-01

    window of the cell cycle, if any, is unknown. Here we show that cyclin A2-Cdk2 binds and phosphorylates Cdc20 in interphase and this inhibits APC/C-Cdc20 activity. Preventing Cdc20 phosphorylation results in pre-mature activation of the APC/C-Cdc20 and several substrates, including cyclin B1 and A2, are...... destabilized which lengthens G2 and slows mitotic entry. Expressing non-degradable cyclin A2 but not cyclin B1 restores mitotic entry in these cells. We have thus uncovered a novel positive feedback loop centred on cyclin A2-Cdk2 inhibition of interphase APC/C-Cdc20 to allow further cyclin A2 accumulation and...

  11. Sistema Distribuido de Gran Escala

    Directory of Open Access Journals (Sweden)

    Fco. Javier Luna Rosas

    2006-01-01

    Full Text Available Este artículo propone una nueva forma de balancear la carga dinámicamente combinando un modelo Fuzzy-Logic en conjunto con el algoritmo distribuido Request for Bids (RB, el trabajo es centrado en el modelo Fuzzy-Logic con el propósito de mejorar el balanceo dinámico de carga en un sistema distribuido a gran escala. El modelo Fuzzy-Logic y RB fueron implementados en una arquitectura para balancear la carga bajo el estándar de CORBA y fueron evaluados contra otras estrategias que son bien conocidas en la literatura usando diferentes métricas de carga y mostramos como bajo ciertas condiciones nuestra estrategia obtiene mejores resultados.

  12. A novel functional polymorphism in the Cdc6 promoter is associated with the risk for hepatocellular carcinoma

    International Nuclear Information System (INIS)

    Cdc6 is essential for DNA replication and its deregulation is involved in carcinogenesis. To date, the biological significance of the polymorphism in Cdc6 promoter is still unknown. In this study, we aimed to evaluate the influence of the Cdc6 -515A>G polymorphism (rs4134994) on the individual's susceptibility to cancer and on the function of Cdc6. The Cdc6 -515A>G polymorphism was genotyped in 387 hepatocellular carcinoma (HCC) and 389 age- and sex-matched healthy subjects. The association between the genotypes and the risk for HCC was then estimated by unconditional logistic regression analysis with adjustment for age, sex and HBV status. Compared with the AA homozygotes, the homozygous GG genotype (adjusted OR = 0.36, 95% confidence interval (CI) = 0.18-0.72, P = 0.004) or the combined AG/GG genotypes (adjusted OR = 0.56, 95% CI = 0.36-0.86, P = 0.008) were statistically significantly associated with the reduced risk for HCC. Moreover, the analysis using luciferase reporter system showed that the G-allelic Cdc6 promoter displayed a decreased transcriptional activity compared with the A-allelic one. These results indicate that the individuals with G allele may have reduced Cdc6 expression and are therefore in reduced risk for HCC. Further investigation using electrophoretic mobility shift assay (EMSA) revealed that the G allele had a stronger binding strength to nuclear protein(s) which might function as negative regulator(s) for Cdc6 transcription. Our findings suggest that the -515A>G polymorphism may affect the Cdc6 promoter binding affinity with nuclear protein(s) and in turn the Cdc6 expression, which consequently modulates the individual's susceptibility to HCC

  13. CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor I with chromatin.

    Science.gov (United States)

    Jeffery, Daniel C B; Kakusho, Naoko; You, Zhiying; Gharib, Marlene; Wyse, Brandon; Drury, Erin; Weinreich, Michael; Thibault, Pierre; Verreault, Alain; Masai, Hisao; Yankulov, Krassimir

    2015-01-01

    Chromatin Assembly Factor I (CAF-I) plays a key role in the replication-coupled assembly of nucleosomes. It is expected that its function is linked to the regulation of the cell cycle, but little detail is available. Current models suggest that CAF-I is recruited to replication forks and to chromatin via an interaction between its Cac1p subunit and the replication sliding clamp, PCNA, and that this interaction is stimulated by the kinase CDC7. Here we show that another kinase, CDC28, phosphorylates Cac1p on serines 94 and 515 in early S phase and regulates its association with chromatin, but not its association with PCNA. Mutations in the Cac1p-phosphorylation sites of CDC28 but not of CDC7 substantially reduce the in vivo phosphorylation of Cac1p. However, mutations in the putative CDC7 target sites on Cac1p reduce its stability. The association of CAF-I with chromatin is impaired in a cdc28-1 mutant and to a lesser extent in a cdc7-1 mutant. In addition, mutations in the Cac1p-phosphorylation sites by both CDC28 and CDC7 reduce gene silencing at the telomeres. We propose that this phosphorylation represents a regulatory step in the recruitment of CAF-I to chromatin in early S phase that is distinct from the association of CAF-I with PCNA. Hence, we implicate CDC28 in the regulation of chromatin reassembly during DNA replication. These findings provide novel mechanistic insights on the links between cell-cycle regulation, DNA replication and chromatin reassembly. PMID:25602519

  14. Sistemas de comunicaciones para vuelos no tripulados

    OpenAIRE

    Correa Vila, Alejandro

    2008-01-01

    La iniciativa ATLANTIDA engloba numerosos estudios de ingeniería con el objetivo de reformar el actual sistema de gestión del tráfico aéreo. Dentro de estos estudios se encuentran diversas investigaciones en el campo de las comunicaciones móviles. La gran variabilidad del entorno aeronáutico promueve el estudio de sistemas de comunicaciones móviles capaces de funcionar en este tipo de entorno. Este proyecto propone un sistema de comunicaciones WiMAX en un entorno aeronáutico con aviones no tr...

  15. Sistemas Avanzados de Asistencia al Conductor

    OpenAIRE

    Jimenez Ruiz, Francisco; Gómez Casado, Oscar; Naranjo Hernández, José Eugenio; Serradilla García, Francisco; López, José María; Martín, Ángel

    2011-01-01

    El control inteligente de vehículos autónomos es uno de los retos actuales más importantes de los Sistemas Inteligentes de Transporte. La aplicación de técnicas de inteligencia artificial para la gestión automática de los actuadores del vehículo permite a los diferentes sistemas avanzados de asistencia al conductor (ADAS) y a los sistemas de conducción autónoma, realizar una gestión de nivel bajo de una manera muy similar a la de los conductores humanos, mejorando la seguridad y el confor...

  16. Share!: Sistema para compartir contenidos multimedia

    OpenAIRE

    Cimas Bravo, Eduardo

    2015-01-01

    Este trabajo consiste en un sistema informático destinado a la compartición de contenidos multimedia entre grupos restringidos de usuarios. Los contenidos pueden ser imágenes, vídeos y audios. Este sistema se compone de dos aplicaciones, una aplicación web y una aplicación móvil para dispositivos Android. La esencia del sistema es proporcionar un mecanismo para compartir rápidamente contenidos multimedia entre usuarios. Los contenidos pueden estar ya en sus dispositivos móviles o ser capturad...

  17. Ensayo sobre el sistema educativo argentino actual

    OpenAIRE

    Melillo, Oscar Roberto; Lenoier, Daniela; Varela, Claudia

    2009-01-01

    En este trabajo de reflexión, intentaremos homologar al Sistema Educativo con la noción de Aparato Psíquico, considerando que ambos sistemas, por separados, se encuentran desgastados, pero contando con otros recursos. Desde este perspectiva diremos en primera instancia, que el Sistema Educativo Argentino, en los últimos años, ha sufrido una serie de cambios, ya sean estos, a nivel de la política educativa, de los cambios socio-económicos, de los dirigentes políticos, así como por la impronta ...

  18. Sistemas agroforestales ¿pasado o futuro?

    OpenAIRE

    Arribas Lumbreras, Teresa; Blas Morente, Alejandro; Barreiro Elorza, Pilar; Hernández Díaz-Ambrona, Carlos Gregorio

    2011-01-01

    Los sistemas agroforestales han sufrido un decrecimiento continuo durante las últimas décadas, en especial en países de la UE debido a la PAC. La estimación para el año 2050 según la FAO indica la necesidad de aumentar considerablemente la cantidad de alimentos, y por tanto dichos sistemas mixtos árbol-cultivo pueden convertirse en técnicas efectivas y rentables de cultivo. Mediante la exposición de dos casos prácticos, en Colombia y España, se describirá cómo los sistemas agroforestales pued...

  19. Sistema de agentes software para vigilancia

    OpenAIRE

    José M. Molina

    2007-01-01

    El Grupo de Investigación de Inteligencia Artificial Aplicada de la UC3M ha desarrollado un sistema de vigilancia basado en la teoría de agentes y multiagentes del área de inteligencia artificial distribuida. El sistema permite que cada fuente de adquisición de datos pueda operar de manera independiente, pero coordinándose con un sistema central. La tecnología mejora el proceso de vigilancia reduciendo la atención humana e introduciendo alarmas automáticas. Se buscan acuerdos de cooperación t...

  20. Sistemas basados en conocimiento usando Prolog

    OpenAIRE

    Zenaida García Valdivia; Isis Bonet Cruz; Maikel León Espinosa; Pedro Y. Piñero Pérez

    2011-01-01

    Se exponen algunos de los resultados alcanzados en la Universidad Central "Marta Abreu" de Las Villas en cuanto al uso de un lenguaje de Programación Lógica (Prolog) en la elaboración de Sistemas Basados en Conocimiento para diferentes áreas del saber como son la medicina, soldadura, educación, sistemas eléctricos de potencia, entre otros. Todos los sistemas elaborados utilizan el paradigma de la programación lógica para la representación del conocimiento de los expertos en los temas en cuest...

  1. Soporte de sistemas para procesos colaborativos

    OpenAIRE

    Grosclaude, Eduardo

    2005-01-01

    Las experiencias de enseñanza-aprendizaje basadas en plataformas colaborativas se ven afectadas por problemas de disponibilidad provocados por diversos modos de falla de los sistemas que los soportan. El concepto de disponibilidad sugiere incorporar a la gestión de los sistemas la prevención y resolución de esos eventos de falla. La identi cación y eliminación de los puntos únicos de falla mediante el patrón básico de la redundancia permite aproximar el comportamiento de los sistemas resilien...

  2. Fewer U.S. Kids Die from Abusive Head Trauma: CDC

    Science.gov (United States)

    ... U.S. Kids Die From Abusive Head Trauma: CDC Parent training helps prevent shaken baby syndrome, other forms of ... agencies that can help." Briggs said that adding behavioral health ... including parent-child interventions that build on strengths and resilience, ...

  3. Potential utility of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism: a case report

    Science.gov (United States)

    Sato, Takeshi; Muroya, Koji; Hanakawa, Junko; Yamashita, Sumimasa; Nozawa, Kumiko; Masudo, Katsuhiko; Yamakawa, Tadashi; Asakura, Yumi; Hasegawa, Tomonobu; Adachi, Masanori

    2016-01-01

    Abstract. We report a Japanese pedigree with familial primary hyperparathyroidism due to a CDC73 mutation. To our knowledge, this is the first report of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism. The proband had severe psychomotor retardation and received laryngotracheal separation surgery. At 19 yr of age, he developed acute pancreatitis. Hypercalcemia (12.2–13.8 mg/dL), elevated levels of intact PTH (86–160 pg/mL), and a tumor detected upon neck ultrasonography led to the diagnosis of primary hyperparathyroidism. Family history and biochemical examinations revealed that three family members (the proband’s mother, elder brother, and maternal grandfather) had primary hyperparathyroidism. We identified a novel heterozygous mutation, c.240delT, p.Glu81Lysfs*28, in the CDC73 gene in three affected family members, excluding the proband’s elder brother who refused genetic testing. Parathyroidectomy for the proband was considered as high-risk, because the tumor was located close to the tracheostomy orifice. After receiving approval from the institutional review board and obtaining the consent, we initiated cinacalcet treatment. At 22 yr of age, treatment with 100 mg of cinacalcet maintained serum calcium levels below 11.0 mg/dL with no apparent side effects. Our report presents the potential efficacy of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism, in particularly inoperative cases. PMID:27507909

  4. Differential impact of diverse anticancer chemotherapeutics on the Cdc25A-degradation checkpoint pathway

    International Nuclear Information System (INIS)

    When exposed to DNA-damaging insults such as ionizing radiation (IR) or ultraviolet light (UV), mammalian cells activate checkpoint pathways to halt cell cycle progression or induce cell death. Here we examined the ability of five commonly used anticancer drugs with different mechanisms of action to activate the Chk1/Chk2-Cdc25A-CDK2/cyclin E cell cycle checkpoint pathway, previously shown to be induced by IR or UV. Whereas exposure of human cells to topoisomerase inhibitors camptothecin, etoposide, or adriamycin resulted in rapid (within 1 h) activation of the pathway including degradation of the Cdc25A phosphatase and inhibition of cyclin E/CDK2 kinase activity, taxol failed to activate this checkpoint even after a prolonged treatment. Unexpectedly, although the alkylating agent cisplatin also induced degradation of Cdc25A (albeit delayed, after 8-12 h), cyclin E/CDK2 activity was elevated and DNA synthesis continued, a phenomena that correlated with increased E2F1 protein levels and consequently enhanced expression of cyclin E. These results reveal a differential impact of various classes of anticancer chemotherapeutics on the Cdc25A-degradation pathway, and indicate that the kinetics of checkpoint induction, and the relative balance of key components within the DNA damage response network may dictate whether the treated cells arrest their cell cycle progression

  5. Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin A

    NARCIS (Netherlands)

    Wolthuis, Rob; Clay-Farrace, Lori; van Zon, Wouter; Yekezare, Mona; Koop, Lars; Ogink, Janneke; Medema, Rene; Pines, Jonathon

    2008-01-01

    Successful mitosis requires the right protein be degraded at the right time. Central to this is the spindle checkpoint that prevents the destruction of securin and cyclin 131 when there are improperly attached chromosomes. The principal target of the checkpoint is Cdc20, which activates the anaphase

  6. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1

    NARCIS (Netherlands)

    Lee, S.B.; Kim, J.J.; Nam, H.J.; Gao, B.; Yin, P.; Qin, B.; Yi, S.Y.; Ham, H.; Evans, D.; Kim, S.H.; Zhang, Jun; Deng, M.; Liu, T.; Zhang, H.; Billadeau, D.D.; Wang, L.; Giaime, E.; Shen, J.; Pang, Y.P.; Jen, J.; Deursen, J.M.A. van; Lou, Z.

    2015-01-01

    Mutations in the E3 ubiquitin ligase Parkin have been linked to familial Parkinson's disease. Parkin has also been implicated in mitosis through mechanisms that are unclear. Here we show that Parkin interacts with anaphase promoting complex/cyclosome (APC/C) coactivators Cdc20 and Cdh1 to mediate th

  7. Cdc42-dependent structural development of auditory supporting cells is required for wound healing at adulthood

    DEFF Research Database (Denmark)

    Anttonen, Tommi; Kirjavainen, Anna; Belevich, Ilya;

    2012-01-01

    of a basolateral membrane protein in the apical domain were observed. These defects and changes in aPKCλ/ι expression suggested that apical polarization is impaired. Following a lesion at adulthood, supporting cells with Cdc42 loss-induced maturational defects collapsed and failed to remodel F...

  8. Cdc42 controls progenitor cell differentiation and beta-catenin turnover in skin

    DEFF Research Database (Denmark)

    Wu, Xunwei; Quondamatteo, Fabio; Lefever, Tine;

    2006-01-01

    Differentiation of skin stem cells into hair follicles (HFs) requires the inhibition of beta-catenin degradation, which is controlled by a complex containing axin and the protein kinase GSK3beta. Using conditional gene targeting in mice, we show now that the small GTPase Cdc42 is crucial for...

  9. 1 in 8 U.S. Workers Has Some Hearing Loss: CDC

    Science.gov (United States)

    ... gov/news/fullstory_158434.html 1 in 8 U.S. Workers Has Some Hearing Loss: CDC Survey finds ... 21, 2016 (HealthDay News) -- Nearly 13 percent of U.S. workers suffer from at least some hearing loss, ...

  10. Abortion surveillance at CDC: creating public health light out of political heat.

    Science.gov (United States)

    Cates, W; Grimes, D A; Schulz, K F

    2000-07-01

    In the late 1960s, states began to liberalize their abortion laws, and a new era in women's health began. Under the leadership of Jack Smith, the Centers for Disease Control and Prevention (CDC) established a voluntary abortion surveillance system that provided the first nationwide information on the numbers and characteristics of women having abortions. Studies of abortion morbidity done by the CDC revealed that suction curettage was safer than sharp curettage, local anesthesia was safer than general anesthesia, free-standing clinics were safer than hospitals, and dilation and evacuation (D&E) was safer than the alternative of labor induction for early second-trimester abortions. This evidence, which contradicted traditional medical tenets, rapidly changed the practice of abortion in the United States. CDC also established a surveillance system for abortion deaths. This demonstrated a rapid improvement in the safety of abortion in the early 1970s. Lessons learned from mortality investigations helped to change practice as well.Today, more is known about the epidemiology of abortion than any other operation in the history of medicine. In the midst of strident debate over the abortion issue, CDC abortion surveillance data have helped to guide judicial rulings, legislative actions, and Surgeon General's reports, which have supported safer choices for women of reproductive age. When medical historians of the future look back on this century, the increasing availability of safe, legal abortion will stand out as a public health triumph. PMID:10863125

  11. Potential utility of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism: a case report.

    Science.gov (United States)

    Sato, Takeshi; Muroya, Koji; Hanakawa, Junko; Yamashita, Sumimasa; Nozawa, Kumiko; Masudo, Katsuhiko; Yamakawa, Tadashi; Asakura, Yumi; Hasegawa, Tomonobu; Adachi, Masanori

    2016-07-01

    We report a Japanese pedigree with familial primary hyperparathyroidism due to a CDC73 mutation. To our knowledge, this is the first report of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism. The proband had severe psychomotor retardation and received laryngotracheal separation surgery. At 19 yr of age, he developed acute pancreatitis. Hypercalcemia (12.2-13.8 mg/dL), elevated levels of intact PTH (86-160 pg/mL), and a tumor detected upon neck ultrasonography led to the diagnosis of primary hyperparathyroidism. Family history and biochemical examinations revealed that three family members (the proband's mother, elder brother, and maternal grandfather) had primary hyperparathyroidism. We identified a novel heterozygous mutation, c.240delT, p.Glu81Lysfs*28, in the CDC73 gene in three affected family members, excluding the proband's elder brother who refused genetic testing. Parathyroidectomy for the proband was considered as high-risk, because the tumor was located close to the tracheostomy orifice. After receiving approval from the institutional review board and obtaining the consent, we initiated cinacalcet treatment. At 22 yr of age, treatment with 100 mg of cinacalcet maintained serum calcium levels below 11.0 mg/dL with no apparent side effects. Our report presents the potential efficacy of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism, in particularly inoperative cases. PMID:27507909

  12. Using Evidence-Based Parenting Programs to Advance CDC Efforts in Child Maltreatment Prevention. Research Brief

    Science.gov (United States)

    Valle, Linda Anne; Whitaker, Daniel J.; Lutzker, John R.; Filene, Jill H.; Wyatt, Jennifer M.; Cephas, Kendell C.; Hoover, D. Michele

    2004-01-01

    The Centers for Disease Control and Prevention (CDC) recognize child maltreatment as a serious public health problem with extensive short- and long-term health effects. In addition to the immediate physical and emotional effects of maltreatment, children who have experienced abuse and neglect are at increased risk of adverse health effects and…

  13. CDC Vital Signs–Think Sepsis. Time Matters.

    Centers for Disease Control (CDC) Podcasts

    2016-08-23

    This podcast is based on the August 2016 CDC Vital Signs report. Sepsis is a medical emergency and can happen quickly. Learn the signs of sepsis and how to prevent it.  Created: 8/23/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 8/23/2016.

  14. 13 CFR 120.826 - Basic requirements for operating a CDC.

    Science.gov (United States)

    2010-01-01

    ... Board of the American Institute of Certified Public Accountants (AICPA). The auditor must be independent, as defined by the AICPA, of the CDC. Annually, the auditor must issue an opinion as to the fairness... to SBA must be reviewed by an independent CPA in accordance with GAAP. (d) Auditor...

  15. Sequencing analysis of mutant allele cdc28-srm of protein kinase CDC28 and molecular dynamics study of glycine-rich loop in wild-type and mutant allele G16S of CDK2 as model

    International Nuclear Information System (INIS)

    The central role that cyclin-dependent kinases play in the timing of cell division and the high incidence of genetic alteration of CDKs or deregulation of CDK inhibitors in a number of cancers make CDC28 of the yeast Saccharomyces cerevisiae a very attractive model for studies of mechanisms of CDK regulation. Earlier it was found that certain gene mutations including cdc28-srm affect cell cycle progression, maintenance of different genetic structures and increase cell sensitivity to ionizing radiation. A cdc28-srm mutation is not a temperature-sensitive mutation and differs from the known cdc28-ts mutations because it has the evident phenotypic manifestations at 30 deg C. Sequencing analysis of cdc28-srm revealed a single nucleotide substitution G20S. This is a third glycine in a conserved sequence GxGxxG in the G-rich loop positioned opposite the activation T-loop. Despite its demonstrated importance, the role of the G-loop has remained unclear. The crystal structure of the human CDK2 has served as a model for the catalytic core of other CDKs, including CDC28. Nanoseconds long molecular dynamics (MD) trajectories of the CDK2/ATP complex were analyzed. The MD simulations of CDK2-G16S (CDC28-G20S) substitution show conformational changes of CDK2 structure resulting in the moving of the G-loop away from ATP and a new rearrangement of amino acids in the T-loop

  16. Cdc20 is critical for meiosis I and fertility of female mice.

    Directory of Open Access Journals (Sweden)

    Fang Jin

    2010-09-01

    Full Text Available Chromosome missegregation in germ cells is an important cause of unexplained infertility, miscarriages, and congenital birth defects in humans. However, the molecular defects that lead to production of aneuploid gametes are largely unknown. Cdc20, the activating subunit of the anaphase-promoting complex/cyclosome (APC/C, initiates sister-chromatid separation by ordering the destruction of two key anaphase inhibitors, cyclin B1 and securin, at the transition from metaphase to anaphase. The physiological significance and full repertoire of functions of mammalian Cdc20 are unclear at present, mainly because of the essential nature of this protein in cell cycle progression. To bypass this problem we generated hypomorphic mice that express low amounts of Cdc20. These mice are healthy and have a normal lifespan, but females produce either no or very few offspring, despite normal folliculogenesis and fertilization rates. When mated with wild-type males, hypomorphic females yield nearly normal numbers of fertilized eggs, but as these embryos develop, they become malformed and rarely reach the blastocyst stage. In exploring the underlying mechanism, we uncover that the vast majority of these embryos have abnormal chromosome numbers, primarily due to chromosome lagging and chromosome misalignment during meiosis I in the oocyte. Furthermore, cyclin B1, cyclin A2, and securin are inefficiently degraded in metaphase I; and anaphase I onset is markedly delayed. These results demonstrate that the physiologically effective threshold level of Cdc20 is high for female meiosis I and identify Cdc20 hypomorphism as a mechanism for chromosome missegregation and formation of aneuploid gametes.

  17. Sistemas basados en conocimiento usando Prolog

    Directory of Open Access Journals (Sweden)

    Zenaida García Valdivia

    2011-10-01

    Full Text Available Se exponen algunos de los resultados alcanzados en la Universidad Central "Marta Abreu" de Las Villas en cuanto al uso de un lenguaje de Programación Lógica (Prolog en la elaboración de Sistemas Basados en Conocimiento para diferentes áreas del saber como son la medicina, soldadura, educación, sistemas eléctricos de potencia, entre otros. Todos los sistemas elaborados utilizan el paradigma de la programación lógica para la representación del conocimiento de los expertos en los temas en cuestión y además hacen uso de entornos agradables a los usuarios a través interfaces visuales elaboradas en ambientes de programación para súper lenguajes ó utilizan un conjunto de herramientas para la elaboración de sistemas sobre la Web

  18. El Sistema Nacional de Cualificaciones Profesionales

    OpenAIRE

    Brezmes Arceredillo, Ricardo

    2014-01-01

    El trabajo aborda el Sistema Nacional de Cualificaciones y Formación Profesional desgranando anteriormente conceptos básicos para su entendimiento como son las competencias laborales y profesionales, las cualificaciones profesionales y el Catálogo Nacional de Cualificaciones Profesionales entre otros. Se trata de analizar estos conceptos y reflexionar sobre ellos en las conclusiones de trabajo para así extraer una idea de lo que el Sistema Nacional de Cualificaciones y Formació...

  19. Functional mapping of the fission yeast DNA polymerase δ B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis

    Directory of Open Access Journals (Sweden)

    Gray Fiona C

    2009-08-01

    Full Text Available Abstract Background DNA polymerase δ plays an essential role in chromosomal DNA replication in eukaryotic cells, being responsible for synthesising the bulk of the lagging strand. In fission yeast, Pol δ is a heterotetrameric enzyme comprising four evolutionarily well-conserved proteins: the catalytic subunit Pol3 and three smaller subunits Cdc1, Cdc27 and Cdm1. Pol3 binds directly to the B-subunit, Cdc1, which in turn binds the C-subunit, Cdc27. Human Pol δ comprises the same four subunits, and the crystal structure was recently reported of a complex of human p50 and the N-terminal domain of p66, the human orthologues of Cdc1 and Cdc27, respectively. Results To gain insights into the structure and function of Cdc1, random and directed mutagenesis techniques were used to create a collection of thirty alleles encoding mutant Cdc1 proteins. Each allele was tested for function in fission yeast and for binding of the altered protein to Pol3 and Cdc27 using the two-hybrid system. Additionally, the locations of the amino acid changes in each protein were mapped onto the three-dimensional structure of human p50. The results obtained from these studies identify amino acid residues and regions within the Cdc1 protein that are essential for interaction with Pol3 and Cdc27 and for in vivo function. Mutations specifically defective in Pol3-Cdc1 interactions allow the identification of a possible Pol3 binding surface on Cdc1. Conclusion In the absence of a three-dimensional structure of the entire Pol δ complex, the results of this study highlight regions in Cdc1 that are vital for protein function in vivo and provide valuable clues to possible protein-protein interaction surfaces on the Cdc1 protein that will be important targets for further study.

  20. The use of the processor communication of the CDC-1604A/1 and CDC-1604A/2 computers for observation of scanning process on the HPD device by means of display

    International Nuclear Information System (INIS)

    On a particular example of film data processing from the Dubna Magnetic Spectrometer the possibility is shown for using the communication between CDC-1604A/1 and CDC-1604A/2 computers for the joint working of two logically communicated programs. Combination of HPD and graphic display software by means of the processors communication channel provides an opportunity to have a visual display of the film during its scanning, without restrictions on the time of processing. Thus, using the communication between two computers one can gain conditions for handling new tasks unrealizable with one CDC-1604A computer

  1. FLIM FRET Visualization of Cdc42 Activation by Netrin-1 in Embryonic Spinal Commissural Neuron Growth Cones

    Science.gov (United States)

    Rappaz, Benjamin; Lai Wing Sun, Karen; Correia, James P.; Wiseman, Paul W.; Kennedy, Timothy E.

    2016-01-01

    Netrin-1 is an essential extracellular chemoattractant that signals through its receptor DCC to guide commissural axon extension in the embryonic spinal cord. DCC directs the organization of F-actin in growth cones by activating an intracellular protein complex that includes the Rho GTPase Cdc42, a critical regulator of cell polarity and directional migration. To address the spatial distribution of signaling events downstream of netrin-1, we expressed the FRET biosensor Raichu-Cdc42 in cultured embryonic rat spinal commissural neurons. Using FLIM-FRET imaging we detected rapid activation of Cdc42 in neuronal growth cones following application of netrin-1. Investigating the signaling mechanisms that control Cdc42 activation by netrin-1, we demonstrate that netrin-1 rapidly enriches DCC at the leading edge of commissural neuron growth cones and that netrin-1 induced activation of Cdc42 in the growth cone is blocked by inhibiting src family kinase signaling. These findings reveal the activation of Cdc42 in embryonic spinal commissural axon growth cones and support the conclusion that src family kinase activation downstream of DCC is required for Cdc42 activation by netrin-1. PMID:27482713

  2. An ALS disease mutation in Cdc48/p97 impairs 20S proteasome binding and proteolytic communication.

    Science.gov (United States)

    Barthelme, Dominik; Jauregui, Ruben; Sauer, Robert T

    2015-09-01

    Cdc48 (also known as p97 or VCP) is an essential and highly abundant, double-ring AAA+ ATPase, which is ubiquitous in archaea and eukaryotes. In archaea, Cdc48 ring hexamers play a direct role in quality control by unfolding and translocating protein substrates into the degradation chamber of the 20S proteasome. Whether Cdc48 and 20S cooperate directly in protein degradation in eukaryotic cells is unclear. Two regions of Cdc48 are important for 20S binding, the pore-2 loop at the bottom of the D2 AAA+ ring and a C-terminal tripeptide. Here, we identify an aspartic acid in the pore-2 loop as an important element in 20S recognition. Importantly, mutation of this aspartate in human Cdc48 has been linked to familial amyotrophic lateral sclerosis (ALS). In archaeal or human Cdc48 variants, we find that mutation of this pore-2 residue impairs 20S binding and proteolytic communication but does not affect the stability of the hexamer or rates of ATP hydrolysis and protein unfolding. These results suggest that human Cdc48 interacts functionally with the 20S proteasome. PMID:26134898

  3. Gerencia de sistemas conductuales y cambio organizacional

    Directory of Open Access Journals (Sweden)

    Guillermo E. Yáber

    1998-01-01

    Full Text Available La gerencia de sistemas conductuales es una tecnología basada en un enfoque científico para el análisis del comportamiento en sistemas humanos. Esta tecnología ayuda a las organizaciones a definir y lograr sus metas. Consiste en el análisis, especificación, diseño, implementación, evaluación y reciclaje de sistemas de motivación humana que optimizan el desempeño y desarrollo a nivel del sistema total, departamental e individual de la organización. Utiliza tres modelos: El diseño de sistemas dirigidos hacia la meta, el análisis de «Tres Contingencias» y la gerencia del desempeño para el cambio cultural. Este artículo incluye la descripción del marco teórico y metodológico de la gerencia de sistemas conductuales y su aplicación en las áreas de servicios y producción.

  4. Aplicação do Sistema de Inferência Nebuloso na Determinação de Medicina Preventiva no Sistema de Saúde Complementar

    Directory of Open Access Journals (Sweden)

    Igor Barone de Medeiros

    2006-12-01

    Full Text Available A aplicação do Sistema de Inferência Nebuloso foi realizada na tentativa de direcionar os custos médicos de operadoras de planos de saúde mediante o estudo de variáveis, previamente, selecionadas por grupos médicos de atuação no mercado. Esta medida irá influenciar as decisões pós entrevista qualificada através do mapeamento do risco dos novos clientes. Este estudo tangibiliza a atual situação do mercado de saúde privada no Brasil, tendo como premissa os escassos recursos da Saúde Pública. Em conseqüência há a tentativa do governo em atender a todos de maneira igualitária, contudo os benefícios a serem oferecidos são, infelizmente, segmentados por não haver recursos para toda a população. O PIB no Brasil equivale a 60% do investimento norte americano em saúde, fonte do relatório do Banco Mundial de 1993, o que representa uma longa jornada de trabalho de nossa economia para o aumento da viabilidade de sobrevivência neste mercado que esta cada vez mais acirrada devido a competição que se encontra o setor e saúde complementar. O objetivo central é restaurar a capacidade comercial deste mercado engessado. A organização de pensamentos dos administradores de saúde (muitos são médicos ainda está ao redor dos procedimentos médicos, pois são eles que alimentam economicamente os Hospitais. Seguindo este foco, são apresentadas vertentes que caracterizam a prevenção como a maior fomentadora de recursos para ambos, operadoras e hospitais, tendo em vista a distribuição dos custos médicos e conseqüentemente o aumento da capacidade dos hospitais em absorver novas técnicas de tratamento, garantindo maior qualidade de vida para os clientes, além de abrir portas para novas parcerias.

  5. Distribuição da altura uterina ao longo da gestação em uma coorte brasileira: comparação com a curva de referência do Centro Latino-Americano de Perinatologia Distribution of uterine height during pregnancy in a Brazilian cohort: comparison with the reference curve of the Centro Latino-Americano de Perinatologia

    Directory of Open Access Journals (Sweden)

    Maria Lúcia Rocha Oppermann

    2006-09-01

    Full Text Available OBJETIVOS: descrever, em gestantes do Estudo Brasileiro do Diabetes Gestacional (EBDG, a distribuição da altura uterina de acordo com a idade gestacional e validar a curva do Centro Latino-Americano de Perinatologia (CLAP, curva de referência para a predição de anormalidades do crescimento fetal. MÉTODOS: o EBDG é uma coorte de 5564 gestantes, com mais de 19 anos, seguidas até e após o parto. Entrevistas e medidas antropométricas padronizadas foram feitas no arrolamento entre a 20ª e a 28ª semana. Os prontuários foram revisados segundo protocolo padronizado, abrangendo os períodos de pré-natal e parto. As análises referem-se a 3539 gestantes com datação da gravidez confirmada por ultra-sonografia. Determinamos as propriedades diagnósticas dos percentis 10 e 90 de altura uterina de ambas as curvas (EBDG e CLAP como indicadores de anormalidade no peso neonatal. RESULTADOS: as medidas de altura uterina no EBDG foram maiores que as do CLAP em todas as semanas de gestação (1 a 4 cm e 2 a 6 cm, respectivamente, nos percentis 10 e 90. O percentil 10 do CLAP identificou como pequenas as medidas uterinas de 0,3 a 1,7% das gestantes brasileiras, ao passo que o percentil 90 classificou como grandes as medidas uterinas de 42 a 57% das brasileiras. A sensibilidade do percentil 10 do CLAP para predizer recém-nascidos pequenos para a idade gestacional variou de 0,8 a 6% e a especificidade do percentil 90 para predizer grandes para a idade gestacional, de 46 a 61%. CONCLUSÕES: a curva de referência do CLAP não reflete o padrão de crescimento uterino das gestantes brasileiras, limitando sua capacidade de identificar anormalidades de crescimento fetal, especialmente a restrição de crescimento.PURPOSE: to describe, in participants of the Brazilian Study of Gestational Diabetes (EBDG, the percentile distribution of uterine height by gestational age and to validate the use of percentiles of the chart derived by the "Centro Latino-Americano

  6. The Rho GTPase Cdc42 regulates hair cell planar polarity and cellular patterning in the developing cochlea

    Directory of Open Access Journals (Sweden)

    Anna Kirjavainen

    2015-03-01

    Full Text Available Hair cells of the organ of Corti (OC of the cochlea exhibit distinct planar polarity, both at the tissue and cellular level. Planar polarity at tissue level is manifested as uniform orientation of the hair cell stereociliary bundles. Hair cell intrinsic polarity is defined as structural hair bundle asymmetry; positioning of the kinocilium/basal body complex at the vertex of the V-shaped bundle. Consistent with strong apical polarity, the hair cell apex displays prominent actin and microtubule cytoskeletons. The Rho GTPase Cdc42 regulates cytoskeletal dynamics and polarization of various cell types, and, thus, serves as a candidate regulator of hair cell polarity. We have here induced Cdc42 inactivation in the late-embryonic OC. We show the role of Cdc42 in the establishment of planar polarity of hair cells and in cellular patterning. Abnormal planar polarity was displayed as disturbances in hair bundle orientation and morphology and in kinocilium/basal body positioning. These defects were accompanied by a disorganized cell-surface microtubule network. Atypical protein kinase C (aPKC, a putative Cdc42 effector, colocalized with Cdc42 at the hair cell apex, and aPKC expression was altered upon Cdc42 depletion. Our data suggest that Cdc42 together with aPKC is part of the machinery establishing hair cell planar polarity and that Cdc42 acts on polarity through the cell-surface microtubule network. The data also suggest that defects in apical polarization are influenced by disturbed cellular patterning in the OC. In addition, our data demonstrates that Cdc42 is required for stereociliogenesis in the immature cochlea.

  7. Genetic deletion of cdc42 reveals a crucial role for astrocyte recruitment to the injury site in vitro and in vivo

    DEFF Research Database (Denmark)

    Robel, Stefanie; Bardehle, Sophia; Lepier, Alexandra; Brakebusch, Cord; Götz, Magdalena

    2011-01-01

    signals, the small RhoGTPase Cdc42, selectively in mouse astrocytes in vitro and in vivo. We used an in vitro scratch assay as a minimal wounding model and found that astrocytes lacking Cdc42 (Cdc42Δ) were still able to form protrusions, although in a nonoriented way. Consequently, they failed to migrate...... in a directed manner toward the scratch. When animals were injured in vivo through a stab wound, Cdc42Δ astrocytes developed protrusions properly oriented toward the lesion, but the number of astrocytes recruited to the lesion site was significantly reduced. Surprisingly, however, lesions in Cdc42Δ...

  8. Cdc25B Dual-Specificity Phosphatase Inhibitors Identified in a High-Throughput Screen of the NIH Compound Library

    OpenAIRE

    Johnston, Paul A.; Foster, Caleb A.; Tierno, Marni Brisson; Shun, Tong Ying; Shinde, Sunita N.; Paquette, William D.; Brummond, Kay M.; Wipf, Peter; Lazo, John S.

    2009-01-01

    The University of Pittsburgh Molecular Library Screening Center (Pittsburgh, PA) conducted a screen with the National Institutes of Health compound library for inhibitors of in vitro cell division cycle 25 protein (Cdc25) B activity during the pilot phase of the Molecular Library Screening Center Network. Seventy-nine (0.12%) of the 65,239 compounds screened at 10 μM met the active criterion of ≥50% inhibition of Cdc25B activity, and 25 (31.6%) of these were confirmed as Cdc25B inhibitors wit...

  9. The Tea4-PP1 landmark promotes local growth by dual Cdc42 GEF recruitment and GAP exclusion.

    OpenAIRE

    Kokkoris K.; Gallo Castro D.; Martin S.G.

    2014-01-01

    Cell polarization relies on small GTPases, such as Cdc42, which can break symmetry through self-organizing principles, and landmarks that define the axis of polarity. In fission yeast, microtubules deliver the Tea1-Tea4 complex to mark cell poles for growth, but how this complex activates Cdc42 is unknown. Here, we show that ectopic targeting of Tea4 to cell sides promotes the local activation of Cdc42 and cell growth. This activity requires that Tea4 binds the type I phosphatase (PP1) cataly...

  10. Cogeneration Systems; Sistemas de Cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez M, Manuel F; Huante P, Liborio; Romo M, Cesar A [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2006-07-01

    The present article deals on relevant aspects on the subject of cogeneration within the Mexican territorial limits. In the first place it is presented the role of Mexico in terms of its cogeneration potential, the type of service that has obtained from this predominant modality of cogeneration for self-supplying, the most propitious sectors to develop it, its legislations on the matter, the projects made for the implementation of cogeneration plants, as well as the existing cogeneration schemes for its respective optimization proposals. Without leaving out the analysis on the different types of evaluation on the efficiency of cogeneration systems and the aspects to consider for the election of a generation cycle. [Spanish] El presente articulo trata sobre aspectos relevantes en materia de cogeneracion dentro de los limites territoriales de la nacion mexicana. Se muestra en primer lugar el papel de Mexico en terminos de su potencial de cogeneracion, el tipo de servicio que ha obtenido de esta predominantemente (modalidad de cogeneracion para autoabastecimiento), los sectores mas propicios para desarrollarla, sus legislaciones al respecto, los proyectos realizados para la implementacion de plantas de cogeneracion, asi como los esquemas de cogeneracion existentes con sus respectivas propuestas de optimizacion. Sin dejar de lado el analisis sobre los distintos tipos de evaluacion de la eficiencia de sistemas de cogeneracion y los aspectos a considerar para la eleccion de un ciclo de generacion.

  11. PP2A(Cdc55)'s role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function.

    Science.gov (United States)

    Kerr, Gary W; Wong, Jin Huei; Arumugam, Prakash

    2016-01-01

    PP2A(Cdc55) is a highly conserved serine-threonine protein phosphatase that is involved in diverse cellular processes. In budding yeast, meiotic cells lacking PP2A(Cdc55) activity undergo a premature exit from meiosis I which results in a failure to form bipolar spindles and divide nuclei. This defect is largely due to its role in negatively regulating the Cdc Fourteen Early Anaphase Release (FEAR) pathway. PP2A(Cdc55) prevents nucleolar release of the Cdk (Cyclin-dependent kinase)-antagonising phosphatase Cdc14 by counteracting phosphorylation of the nucleolar protein Net1 by Cdk. CDC55 was identified in a genetic screen for monopolins performed by isolating suppressors of spo11Δ spo12Δ lethality suggesting that Cdc55 might have a role in meiotic chromosome segregation. We investigated this possibility by isolating cdc55 alleles that suppress spo11Δ spo12Δ lethality and show that this suppression is independent of PP2A(Cdc55)'s FEAR function. Although the suppressor mutations in cdc55 affect reductional chromosome segregation in the absence of recombination, they have no effect on chromosome segregation during wild type meiosis. We suggest that Cdc55 is required for reductional chromosome segregation during achiasmate meiosis and this is independent of its FEAR function. PMID:27455870

  12. Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.

    Science.gov (United States)

    Labbe, J C; Picard, A; Peaucellier, G; Cavadore, J C; Nurse, P; Doree, M

    1989-04-21

    MPF extracted from starfish oocytes copurifies with an M phase-specific H1 histone kinase encoded by a homolog of the fission yeast cell cycle control gene cdc2+. The most purified preparations contain p34cdc2 as the only major protein. Activation of the p34cdc2 kinase is correlated with appearance of the MPF activity both in vivo and in vitro. The increase in protein kinase activity is associated with p34cdc2 dephosphorylation and the decrease in protein kinase activity on leaving M phase with rephosphorylation. Microinjection of a peptide perfectly conserved in p34cdc2 from yeast to humans induces meiotic maturation, suggesting that an inhibitory component in G2 arrested oocytes interacts with this region of the p34cdc2 kinase. We propose that initiation of M phase is brought about by the dephosphorylation of p34cdc2, leading to increase in its protein kinase activity. PMID:2649251

  13. Prominin-2 expression increases protrusions, decreases caveolae and inhibits Cdc42 dependent fluid phase endocytosis

    International Nuclear Information System (INIS)

    Highlights: •Prominin-2 expression induced protrusions that co-localized with lipid raft markers. •Prominin-2 expression decreased caveolae, caveolar endocytosis and increased pCav1. •Prominin-2 expression inhibited fluid phase endocytosis by inactivation of Cdc42. •These endocytic effects can be reversed by adding exogenous cholesterol. •Caveolin1 knockdown restored fluid phase endocytosis in Prominin2 expressing cells. -- Abstract: Background: Membrane protrusions play important roles in biological processes such as cell adhesion, wound healing, migration, and sensing of the external environment. Cell protrusions are a subtype of membrane microdomains composed of cholesterol and sphingolipids, and can be disrupted by cholesterol depletion. Prominins are pentaspan membrane proteins that bind cholesterol and localize to plasma membrane (PM) protrusions. Prominin-1 is of great interest as a marker for stem and cancer cells, while Prominin-2 (Prom2) is reportedly restricted to epithelial cells. Aim: To characterize the effects of Prom-2 expression on PM microdomain organization. Methods: Prom2-fluorescent protein was transfected in human skin fibroblasts (HSF) and Chinese hamster ovary (CHO) cells for PM raft and endocytic studies. Caveolae at PM were visualized using transmission electron microscopy. Cdc42 activation was measured and caveolin-1 knockdown was performed using siRNAs. Results: Prom2 expression in HSF and CHO cells caused extensive Prom2-positive protrusions that co-localized with lipid raft markers. Prom2 expression significantly decreased caveolae at the PM, reduced caveolar endocytosis and increased caveolin-1 phosphorylation. Prom2 expression also inhibited Cdc42-dependent fluid phase endocytosis via decreased Cdc42 activation. Effects on endocytosis were reversed by addition of cholesterol. Knockdown of caveolin-1 by siRNA restored Cdc42 dependent fluid phase endocytosis in Prom2-expressing cells. Conclusions: Prom2 protrusions primarily

  14. Prominin-2 expression increases protrusions, decreases caveolae and inhibits Cdc42 dependent fluid phase endocytosis

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Raman Deep, E-mail: Takhter.Ramandeep@mayo.edu; Schroeder, Andreas S.; Scheffer, Luana; Holicky, Eileen L.; Wheatley, Christine L.; Marks, David L., E-mail: Marks.david@mayo.edu; Pagano, Richard E.

    2013-05-10

    Highlights: •Prominin-2 expression induced protrusions that co-localized with lipid raft markers. •Prominin-2 expression decreased caveolae, caveolar endocytosis and increased pCav1. •Prominin-2 expression inhibited fluid phase endocytosis by inactivation of Cdc42. •These endocytic effects can be reversed by adding exogenous cholesterol. •Caveolin1 knockdown restored fluid phase endocytosis in Prominin2 expressing cells. -- Abstract: Background: Membrane protrusions play important roles in biological processes such as cell adhesion, wound healing, migration, and sensing of the external environment. Cell protrusions are a subtype of membrane microdomains composed of cholesterol and sphingolipids, and can be disrupted by cholesterol depletion. Prominins are pentaspan membrane proteins that bind cholesterol and localize to plasma membrane (PM) protrusions. Prominin-1 is of great interest as a marker for stem and cancer cells, while Prominin-2 (Prom2) is reportedly restricted to epithelial cells. Aim: To characterize the effects of Prom-2 expression on PM microdomain organization. Methods: Prom2-fluorescent protein was transfected in human skin fibroblasts (HSF) and Chinese hamster ovary (CHO) cells for PM raft and endocytic studies. Caveolae at PM were visualized using transmission electron microscopy. Cdc42 activation was measured and caveolin-1 knockdown was performed using siRNAs. Results: Prom2 expression in HSF and CHO cells caused extensive Prom2-positive protrusions that co-localized with lipid raft markers. Prom2 expression significantly decreased caveolae at the PM, reduced caveolar endocytosis and increased caveolin-1 phosphorylation. Prom2 expression also inhibited Cdc42-dependent fluid phase endocytosis via decreased Cdc42 activation. Effects on endocytosis were reversed by addition of cholesterol. Knockdown of caveolin-1 by siRNA restored Cdc42 dependent fluid phase endocytosis in Prom2-expressing cells. Conclusions: Prom2 protrusions primarily

  15. MRG15 activates the cdc2 promoter via histone acetylation in human cells

    International Nuclear Information System (INIS)

    Chromatin remodeling is required for transcriptional activation and repression. MRG15 (MORF4L1), a chromatin modulator, is a highly conserved protein and is present in complexes containing histone acetyltransferases (HATs) as well as histone deacetylases (HDACs). Loss of expression of MRG15 in mice and Drosophila results in embryonic lethality and fibroblast and neural stem/progenitor cells cultured from Mrg15 null mouse embryos exhibit marked proliferative defects when compared with wild type cells. To determine the role of MRG15 in cell cycle progression we performed chromatin immunoprecipitation with an antibody to MRG15 on normal human fibroblasts as they entered the cell cycle from a quiescent state, and analyzed various cell cycle gene promoters. The results demonstrated a 3-fold increase in MRG15 occupancy at the cdc2 promoter during S phase of the cell cycle and a concomitant increase in acetylated histone H4. H4 lysine 12 was acetylated at 24 h post-serum stimulation while there was no change in acetylation of lysine 16. HDAC1 and 2 were decreased at this promoter during cell cycle progression. Over-expression of MRG15 in HeLa cells activated a cdc2 promoter-reporter construct in a dose-dependent manner, whereas knockdown of MRG15 resulted in decreased promoter activity. In order to implicate HAT activity, we treated cells with the HAT inhibitor anacardic acid and determined that HAT inhibition results in loss of expression of cdc2 mRNA. Further, chromatin immunoprecipitation with Tip60 localizes the protein to the same 110 bp stretch of the cdc2 promoter pulled down by MRG15. Additionally, we determined that cotransfection of MRG15 with the known associated HAT Tip60 had a cooperative effect in activating the cdc2 promoter. These results suggest that MRG15 is acting in a HAT complex involving Tip60 to modify chromatin via acetylation of histone H4 at the cdc2 promoter to activate transcription.

  16. Regulación neuroendocrina del sistema inmune

    OpenAIRE

    Caballero Hernández, Diana; TAMEZ GUERRA, REYES S.; RODRÍGUEZ PADILLA, CRISTINA; Tamez Guerra, Patricia; Richard J. Weber; Gómez Flores, Ricardo

    2001-01-01

    El sistema inmune recibe señales del sistema nervioso central (cerebro) vía el sistema nervioso autónomo y el sistema endocrino. El sistema inmune, a su vez, envía información al cerebro vía citocinas. Este sistema de retroalimentación es vital para el funcionamiento adecuado del organismo en situaciones normales, y en aquellas en las que la homeostasis se ve perturbada, como en casos de estrés, consumo de drogas (terapéuticas o de abuso), enfermedades infecciosas y...

  17. Isolation and characterization of new alleles of the cyclin-dependent kinase gene CDC28 with cyclin-specific functional and biochemical defects.

    Science.gov (United States)

    Levine, K; Oehlen, L J; Cross, F R

    1998-01-01

    The G1 cyclin Cln2 negatively regulates the mating-factor pathway. In a genetic screen to identify factors required for this regulation, we identified an allele of CDC28 (cdc28-csr1) that blocked this function of Cln2. Cln2 immunoprecipitated from cdc28-csr1 cells was completely defective in histone H1 kinase activity, due to defects in Cdc28 binding and activation by Cln2. In contrast, Clb2-associated H1 kinase and Cdc28 binding was normal in immunoprecipitates from these cells. cdc28-csr1 was significantly deficient in other aspects of genetic interaction with Cln2. The cdc28-csr1 mutation was determined to be Q188P, in the T loop distal to most of the probable Cdk-cyclin interaction regions. We performed random mutagenesis of CDC28 to identify additional alleles incapable of causing CLN2-dependent mating-factor resistance but capable of complementing cdc28 temperature-sensitive and null alleles. Two such mutants had highly defective Cln2-associated kinase, but, surprisingly, two other mutants had levels of Cln2-associated kinase near to wild-type levels. We performed a complementary screen for CDC28 mutants that could cause efficient Cln2-dependent mating-factor resistance but not complement a cdc28 null allele. Most such mutants were found to alter residues essential for kinase activity; the proteins had little or no associated kinase activity in bulk or in association with Cln2. Several of these mutants also functioned in another assay for CLN2-dependent function not involving the mating-factor pathway, complementing the temperature sensitivity of a cln1 cln3 cdc28-csr1 strain. These results could indicate that Cln2-Cdc28 kinase activity is not directly relevant to some CLN2-mediated functions. Mutants of this sort should be useful in differentiating the function of Cdc28 complexed with different cyclin regulatory subunits. PMID:9418876

  18. Diagnóstico del sistema financiero, la banca comercial y el sistema de portafolios basado en el modelo de sistemas viables

    OpenAIRE

    Beatriz Trueba-Ríos; Isaías Badillo-Piña; Oswaldo Morales-Matamoros

    2012-01-01

    Se presenta en este artículo el desarrollo del diagnóstico para el sistema financiero, la banca comercial y el sistema de portafolios para lo cual se hace uso del modelo de sistemas viables de Beer [1]. El sistema financiero ha sido impactado por movimientos adversos del mercado, lo que lo hace dinámico. Haciendo una revisión minuciosa de las etapas de crisis por las que ha pasado el sistema financiero en forma global, se ha observado que la intervención del factor humano en la toma de decisi...

  19. Manuel J. Peláez (dir., Diccionario de Canonistas y Eclesiasticistas Europeos y Americanos (Tomo I, AV Akademikerverlag GmbH & Co. KG, EAE, Saarbrücken, 2012, 547 págs.

    Directory of Open Access Journals (Sweden)

    Purificación Alcaide-Pulido

    2013-03-01

    Full Text Available ¿Por qué se otorga escasa atención a los canonistas y eclesiasticistas dentro del Derecho canónico y del Derecho eclesiástico? Esta es la cuestión que surge y el motivo de la aparición de la obra Diccionario de canonistas y eclesiasticistas europeos y americanos. Dicha obra aparece como una necesidad científica emanada de la escasa atención que en el ámbito citado se presta a canonistas y a eclesiasticistas, a través de una recopilación de los pensadores más influyentes en las diversas áreas que comprenden el Derecho Canónico y Eclesiástico, a lo largo del último milenio y, por ende, también de interés para iushistoricistas.

  20. Los TLC de Estados Unidos con países latinoamericanos: un modelo de integración económica “superficial” para el continente americano

    Directory of Open Access Journals (Sweden)

    José Julián Tole

    2013-08-01

    Full Text Available El modelo TLC de Estados Unidos se convierte en un instrumento de integración “ideal” para mejorar sus intereses geopolíticos y geoeconómicos en el continente americano, pues facilita la construcción de peldaños o pequeños bloques que mejorarán su peso en la escena internacional y en algunos sectores del comercio internacional. En tal contexto, el objetivo de este trabajo es analizar el modelo “hecho a la medida” por Estados Unidos para regular y profundizar la liberalización comercial, sin considerar de ninguna manera un mayor grado de integración, con los países latinoamericanos.

  1. Los TLC de Estados Unidos con países latinoamericanos: un modelo de integración económica “superficial” para el continente americano

    Directory of Open Access Journals (Sweden)

    José Julián Tole

    2013-07-01

    Full Text Available El modelo TLC de Estados Unidos se convierte en un instrumento de integración “ideal” para mejorar sus intereses geopolíticos y geoeconómicos en el continente americano, pues facilita la construcción de peldaños o pequeños bloques que mejorarán su peso en la escena internacional y en algunos sectores del comercio internacional. En tal contexto, el objetivo de este trabajo es analizar el modelo “hecho a la medida” por Estados Unidos para regular y profundizar la liberalización comercial, sin considerar de ninguna manera un mayor grado de integración, con los países latinoamericanos.

  2. Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts.

    Science.gov (United States)

    Lebensohn, Andres M; Ma, Le; Ho, Hsin-Yi Henry; Kirschner, Marc W

    2006-01-01

    Xenopus egg cytoplasmic extracts have been used to study a variety of complex cellular processes. Given their amenability to biochemical manipulation and physiological balance of regulatory proteins, these extracts are an ideal system to dissect signal transduction pathways leading to actin assembly. We have developed methods to study Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts. In this chapter, we describe detailed procedures to prepare Xenopus egg extracts, Cdc42, and PI(4,5)P2 for use in actin assembly experiments. We also describe a fluorometric pyrene actin assay for quantitative kinetic analysis of actin polymerization and a microscopic rhodamine actin assay for quick measurement of actin rearrangements in extracts. Finally we provide a protocol for immunodepletion of proteins and discuss the use of immunodepletion and rescue experiments for functional analysis of components in the extracts. PMID:16472657

  3. Cdc42/N-WASP signaling links actin dynamics to pancreatic beta cell delamination and differentiation

    DEFF Research Database (Denmark)

    Kesavan, Gokul; Lieven, Oliver; Mamidi, Anant;

    2014-01-01

    Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination and epithelial cell movement within the plane of an epithelium are associated with a change in cellular phenotype. However, how this positional change is linked to differentiat......Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination and epithelial cell movement within the plane of an epithelium are associated with a change in cellular phenotype. However, how this positional change is linked to...... differentiation remains unknown. Using the developing mouse pancreas as a model system, we show that β cell delamination and differentiation are two independent events, which are controlled by Cdc42/N-WASP signaling. Specifically, we show that expression of constitutively active Cdc42 in β cells inhibits β cell...

  4. Ebola in West Africa--CDC's Role in Epidemic Detection, Control, and Prevention.

    Science.gov (United States)

    Frieden, Thomas R; Damon, Inger K

    2015-11-01

    Since Ebola virus disease was identified in West Africa on March 23, 2014, the Centers for Disease Control and Prevention (CDC) has undertaken the most intensive response in the agency's history; >3,000 staff have been involved, including >1,200 deployed to West Africa for >50,000 person workdays. Efforts have included supporting incident management systems in affected countries; mobilizing partners; and strengthening laboratory, epidemiology, contact investigation, health care infection control, communication, and border screening in West Africa, Nigeria, Mali, Senegal, and the United States. All efforts were undertaken as part of national and global response activities with many partner organizations. CDC was able to support community, national, and international health and public health staff to prevent an even worse event. The Ebola virus disease epidemic highlights the need to strengthen national and international systems to detect, respond to, and prevent the spread of future health threats. PMID:26484940

  5. A Decision Processing Algorithm for CDC Location Under Minimum Cost SCM Network

    Science.gov (United States)

    Park, N. K.; Kim, J. Y.; Choi, W. Y.; Tian, Z. M.; Kim, D. J.

    Location of CDC in the matter of network on Supply Chain is becoming on the high concern these days. Present status of methods on CDC has been mainly based on the calculation manually by the spread sheet to achieve the goal of minimum logistics cost. This study is focused on the development of new processing algorithm to overcome the limit of present methods, and examination of the propriety of this algorithm by case study. The algorithm suggested by this study is based on the principle of optimization on the directive GRAPH of SCM model and suggest the algorithm utilizing the traditionally introduced MST, shortest paths finding methods, etc. By the aftermath of this study, it helps to assess suitability of the present on-going SCM network and could be the criterion on the decision-making process for the optimal SCM network building-up for the demand prospect in the future.

  6. 75 FR 4830 - Advisory Committee to the Director (ACD), Centers for Disease Control (CDC) and Prevention-Ethics...

    Science.gov (United States)

    2010-01-29

    ...), Centers for Disease Control (CDC) and Prevention--Ethics Subcommittee (ES) In accordance with section 10(a... health ethics questions and issues arising from programs, scientists, and practitioners. Matter to...

  7. 75 FR 7483 - Advisory Committee to the Director (ACD), Centers for Disease Control (CDC) and Prevention-Ethics...

    Science.gov (United States)

    2010-02-19

    ... February 18, 2010, meeting published at 75 FR 4830, January 29, 2010, is 1 p.m.-5 p.m. FOR FURTHER...), Centers for Disease Control (CDC) and Prevention--Ethics Subcommittee (ES); Correction AGENCY: Centers...

  8. No Drop in Teen Use of Tobacco Products, CDC Says, and E-Cigs May Be Why

    Science.gov (United States)

    ... nih.gov/medlineplus/news/fullstory_158315.html No Drop in Teen Use of Tobacco Products, CDC Says, ... to the study. While there was a significant drop in cigarette smoking between 2011 and 2015, there ...

  9. La morera: una alternativa viable para los sistemas de alimentación animal en el trópico

    Directory of Open Access Journals (Sweden)

    D. García

    2006-01-01

    Full Text Available Se presenta una revisión sobre las principales características de las especies de morera y sus potencialidades como plantas forrajeras en las condiciones tropicales; se hace énfasis en los aspectos relacionados con su taxonomía, distribución geográfica, principales usos, manejo agronómico, composición química, valor nutritivo y principales metabolitos secundarios presentes en su biomasa. Las especies de morera, oriundas del continente euroasiático, pertenecen a la familia Moraceae. En ese sentido, Morus alba y Morus nigra son las mayormente estudiadas por su gran distribución mundial. Desde tiempos ancestrales la morera se ha utilizado en Asia para la alimentación del gusano que produce la seda y los estudios agronómicos realizados en la sericultura han proporcionado numerosas informaciones. Sin embargo, la influencia de los principales factores en la producción de forraje solamente ha sido estudiada en forma intensiva después de su introducción al Continente Americano a partir de 1980. La morera se caracteriza por presentar una sobresaliente producción de biomasa (2-30 tMS/ha/año, contenidos elevados de proteína cruda (>15% MS, adecuada fracción fibrosa (20-40% y digestibilidad entre 70 y 90%. La biomasa contiene agentes pronutricionales (flavonoides, cumarinas, esteroles, saponinas, lectinas y fitoestrógenos. No obstante, los contenidos de estos metabolitos no sobrepasan los niveles críticos establecidos para los rumiantes y solamente los tres últimos grupos presentan potencial antinutricional en monogástricos. En virtud de sus singulares características, las especies de morera constituyen excelentes alternativas alimentarias en los sistemas tropicales de bajos insumos.

  10. Single site suppressors of a fission yeast temperature-sensitive mutant in cdc48 identified by whole genome sequencing.

    Directory of Open Access Journals (Sweden)

    Irina N Marinova

    Full Text Available The protein called p97 in mammals and Cdc48 in budding and fission yeast is a homo-hexameric, ring-shaped, ubiquitin-dependent ATPase complex involved in a range of cellular functions, including protein degradation, vesicle fusion, DNA repair, and cell division. The cdc48+ gene is essential for viability in fission yeast, and point mutations in the human orthologue have been linked to disease. To analyze the function of p97/Cdc48 further, we performed a screen for cold-sensitive suppressors of the temperature-sensitive cdc48-353 fission yeast strain. In total, 29 independent pseudo revertants that had lost the temperature-sensitive growth defect of the cdc48-353 strain were isolated. Of these, 28 had instead acquired a cold-sensitive phenotype. Since the suppressors were all spontaneous mutants, and not the result of mutagenesis induced by chemicals or UV irradiation, we reasoned that the genome sequences of the 29 independent cdc48-353 suppressors were most likely identical with the exception of the acquired suppressor mutations. This prompted us to test if a whole genome sequencing approach would allow us to map the mutations. Indeed genome sequencing unambiguously revealed that the cold-sensitive suppressors were all second site intragenic cdc48 mutants. Projecting these onto the Cdc48 structure revealed that while the original temperature-sensitive G338D mutation is positioned near the central pore in the hexameric ring, the suppressor mutations locate to subunit-subunit and inter-domain boundaries. This suggests that Cdc48-353 is structurally compromized at the restrictive temperature, but re-established in the suppressor mutants. The last suppressor was an extragenic frame shift mutation in the ufd1 gene, which encodes a known Cdc48 co-factor. In conclusion, we show, using a novel whole genome sequencing approach, that Cdc48-353 is structurally compromized at the restrictive temperature, but stabilized in the suppressors.

  11. Activated Cdc42-associated kinase Ack1 promotes prostate cancer progression via androgen receptor tyrosine phosphorylation

    OpenAIRE

    Mahajan, Nupam P.; Liu, Yuanbo; Majumder, Samarpan; Warren, Maria R.; Parker, Carol E.; Mohler, James L.; Earp, H. Shelton; Whang, Young E.

    2007-01-01

    Activation of the androgen receptor (AR) may play a role in androgen-independent progression of prostate cancer. Multiple mechanisms of AR activation, including stimulation by tyrosine kinases, have been postulated. We and others have recently shown involvement of activated Cdc42-associated tyrosine kinase Ack1 in advanced human prostate cancer. Here we provide the molecular basis for interplay between Ack1 and AR in prostate cancer cells. Activated Ack1 promoted androgen-independent growth o...

  12. Cdc42p and Fus2p act together late in yeast cell fusion

    OpenAIRE

    Ydenberg, Casey A.; Stein, Richard A; Rose, Mark D.

    2012-01-01

    Cell fusion is the key event of fertilization that gives rise to the diploid zygote and is a nearly universal aspect of eukaryotic biology. In the yeast Saccharomyces cerevisiae, several mutants have been identified that are defective for cell fusion, and yet the molecular mechanism of this process remains obscure. One obstacle has been that genetic screens have mainly focused on mating-specific factors, whereas the process likely involves housekeeping proteins as well. Here we implicate Cdc4...

  13. Cdc7-Dbf4 Is a Gene-Specific Regulator of Meiotic Transcription in Yeast

    OpenAIRE

    Lo, Hsiao-Chi; Kunz, Ryan C.; Chen, Xiangyu; Marullo, Allison; Steven P Gygi; Hollingsworth, Nancy M.

    2012-01-01

    Meiosis divides the chromosome number of the cell in half by having two rounds of chromosome segregation follow a single round of chromosome duplication. The first meiotic division is unique in that homologous pairs of sister chromatids segregate to opposite poles. Recent work in budding and fission yeast has shown that the cell cycle kinase, Cdc7-Dbf4, is required for many meiosis-specific chromosomal functions necessary for proper disjunction at meiosis I. This work reveals another role for...

  14. CDC28, NETI, and HFII are required for checkpoints in Saccharomyces cerevisiae

    International Nuclear Information System (INIS)

    The involvement of SRM genes selected as genes affecting genetic stability and radiosensitivity in a cell cycle arrest under the action of damaging agents was studied. It was shown that the srm5/cdc28-srm, srm8/netI-srm, and srmI2/hfiI-srm mutations prevent checkpoint activation by DNA damage, particularly the G0/S (srm5, srm8), G1/S (srm5, srm8, srm12), S (srm8, srm12) and S/G2 (srm5) checkpoints. It seems that in budding yeast the CDC28, HFII/ADAI, and NETI genes mediate cellular response induced by DNA damage with checkpoint control. The well-known checkpoint-genes RAD9, RAD17, RAD24, and RAD53, and the genes CDC28, and NETI have been found to belong to one epistasis group named RAD9-group as regards cell sensitivity to γ radiation. An analysis of the radiosensitivity of double mutants has revealed that the mutation cdc-28-srm is hypostatic to each of mutations rad9Δ, and rad24Δ, and additive to rad17Δ. The mutation netI-srm is hypostatic to the mutations rad9Δ but additive to rad17Δ, rad24Δ, and rad53. The mutation hfiI-srm has an additive effect in compound with the mutations rad24Δ and rad9Δ. So, investigations of epistatic interactions have demonstrated a branched RAD9-dependent pathway. The analyzed genes can also participate in a minor mechanism involved in determining cell radiation sensitivity independently of the mentioned RAD9-dependent pathway

  15. Microbiological characteristics of Weeksella virosa (formerly CDC group IIf) isolated from the human genitourinary tract.

    OpenAIRE

    Reina, J; Gil, J.; Salva, F; J. Gomez; Alomar, P

    1990-01-01

    Weeksella virosa (formerly CDC group IIf) is a nonsaccharolytic, oxidase- and catalase-positive, gram-negative rod which is unable to grow on MacConkey agar. At 48 h of incubation on blood or chocolate agar, the colonies present a characteristic appearance: intensely mucoid, adherent, and cream colored as a result of the production of a nondiffusible yellow pigment. This microorganism has been isolated predominantly from the female genitourinary tract, which indicates the opportunity for sexu...

  16. Cdc42 and noncanonical Wnt signal transduction pathways cooperate to promote cell polarity

    OpenAIRE

    Schlessinger, Karni; McManus, Edward J.; Hall, Alan

    2007-01-01

    Scratch-induced disruption of cultured monolayers induces polarity in front row cells that can be visualized by spatially localized polymerization of actin at the front of the cell and reorientation of the centrosome/Golgi to face the leading edge. We previously reported that centrosomal reorientation and microtubule polarization depend on a Cdc42-regulated signal transduction pathway involving activation of the Par6/aPKC complex followed by inhibition of GSK-3β and accumulation of the adenom...

  17. Congenital syphilis after treatment of maternal syphilis with a penicillin regimen exceeding CDC guidelines.

    OpenAIRE

    Conover, C S; Rend, C A; Miller, G B; Schmid, G P

    1998-01-01

    BACKGROUND: Although congenital syphilis usually occurs as a result of a failure to detect and treat syphilis in pregnant women, failures of the currently recommended regimen to prevent congenital syphilis have been reported. CASE: This report describes an infant with congenital syphilis despite maternal treatment with a regimen exceeding current CDC guidelines. CONCLUSION: Regardless of the regimen used to treat syphilis during pregnancy, clinicians should recognize the possibility of occasi...

  18. Addressing Poverty as Risk for Disease: Recommendations from CDC's Consultation on Microenterprise as HIV Prevention

    OpenAIRE

    Stratford, Dale; Mizuno, Yuko; Williams, Kim; Courtenay-Quirk, Cari; O'Leary, Ann

    2008-01-01

    In March 2006, the Centers for Disease Control and Prevention (CDC) convened a consultation meeting to explore microenterprise as a potential human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) prevention intervention. The impulse to link microenterprise with HIV/AIDS prevention was driven by the fact that poverty is a significant factor contributing to the risk for infection. Because increasingly high rates of HIV infection are occurring among women, particularly amo...

  19. Human infections caused by Brevibacterium casei, formerly CDC groups B-1 and B-3.

    OpenAIRE

    Gruner, E; Steigerwalt, A G; Hollis, D G; Weyant, R S; Weaver, R E; Moss, C W; M Daneshvar; J. M. Brown; Brenner, D J

    1994-01-01

    Forty-one clinical strains of CDC coryneform groups B-1 and B-3 were compared biochemically, by analysis of cell wall sugars, amino acids, and cellular fatty acids, and by DNA relatedness to the type strains of Brevibacterium casei, Brevibacterium epidermidis, and Brevibacterium linens. Twenty-two strains were shown to be B. casei, while five other strains formed a phenotypically inseparable genomospecies in the same genus. The remaining isolates were genetically heterogeneous, and most are p...

  20. CDC Signos Vitales: Piense en la septicemia. El tiempo es crucial. (Think Sepsis. Time Matters.)

    Centers for Disease Control (CDC) Podcasts

    2016-08-23

    Este podcast se basa en la edición de agosto del 2016 del informe Signos Vitales de los CDC. La septicemia es una emergencia médica y puede ocurrir rápidamente. Conozca los signos de la septicemia y la forma de prevenirla.  Created: 8/23/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 8/23/2016.

  1. CDC Vital Signs–Motor Vehicle Crash Deaths

    Centers for Disease Control (CDC) Podcasts

    2016-07-06

    This podcast is based on the July 2016 CDC Vital Signs report. In the U.S., about 90 people die in motor vehicle crashes each day and thousands more are injured, resulting in hundreds of millions of dollars in direct medical costs each year. Learn what you can do to stay safe.  Created: 7/6/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 7/6/2016.

  2. CDC Vital Signs-ADHD in Young Children: What You Should Know

    Centers for Disease Control (CDC) Podcasts

    2016-05-03

    This podcast is based on the May 2016 CDC Vital Signs report. For children ages two to five who have ADHD, behavior therapy is recommended before prescribing medicine. This therapy teaches parents ways to improve their child’s behavior and can work as well as medicine, without the risk of side effects.  Created: 5/3/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 5/3/2016.

  3. Remote operation of DOE-1 on the Lawrence Berkeley Laboratory CDC 7600, 6600, and 6400 computers

    Energy Technology Data Exchange (ETDEWEB)

    1978-03-01

    How to run the DOE-1 building energy analysis program on the Lawrence Berkeley Laboratory CDC computers is described. An overview of the LBL operating system and how to run a job on the 7600 are presented. The DOE-1 program, a control card sequence for running the program, and how to store input, output, and intermediate files are discussed. A detailed description is given of the DOE-1 Weather Package.

  4. CDC Vital Signs-Zika and Pregnancy: What You Should Know

    Centers for Disease Control (CDC) Podcasts

    2016-04-01

    This podcast is based on the April 2016 CDC Vital Signs report. A pregnant woman who is infected with Zika virus can pass it to her fetus which is linked to microcephaly, a serious birth defect. This podcast discusses how to protect yourself from Zika virus.  Created: 4/1/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 4/1/2016.

  5. GIT1 promotes lung cancer cell metastasis through modulating Rac1/Cdc42 activity and is associated with poor prognosis

    OpenAIRE

    Chang, Jeng-Shou; Su, Chia-Yi; Yu, Wen-Hsuan; Lee, Wei-Jiunn; Liu, Yu-Peng; Lai, Tsung-Ching; Jan, Yi-Hua; Yang, Yi-Fang; Shen, Chia-Ning; Shew, Jin-Yuh; Lu, Jean; Yang, Chih-Jen; Huang, Ming-Shyan; Lu, Pei-Jung; Lin, Yuan-Feng

    2015-01-01

    G-protein-coupled receptor kinase interacting protein 1 (GIT1) is participated in cell movement activation, which is a fundamental process during tissue development and cancer progression. GIT1/PIX forming a functional protein complex that contributes to Rac1/Cdc42 activation, resulting in increasing cell mobility. Although the importance of Rac1/Cdc42 activation is well documented in cancer aggressiveness, the clinical importance of GIT1 remains largely unknown. Here, we investigated the cli...

  6. The role of nucleotide binding and hydrolysis in the function of the fission yeast cdc18(+) gene product.

    OpenAIRE

    DeRyckere, D; Smith, C.L.; Martin, G S

    1999-01-01

    The fission yeast cdc18(+) gene is required for both initiation of DNA replication and the mitotic checkpoint that normally inhibits mitosis in the absence of DNA replication. The cdc18(+) gene product contains conserved Walker A and B box motifs. Studies of other ATPases have shown that these motifs are required for nucleotide binding and hydrolysis, respectively. We have observed that mutant strains in which either of these motifs is disrupted are inviable. The effects of these mutations we...

  7. Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics*

    OpenAIRE

    Gnad, Florian; Young, Amy; Zhou, Wei; Lyle, Karen; Ong, Christy C.; Matthew P. Stokes; Silva, Jeffrey C.; Belvin, Marcia; Friedman, Lori S.; Koeppen, Hartmut; Minden, Audrey; Hoeflich, Klaus P.

    2013-01-01

    Although K-Ras, Cdc42, and PAK4 signaling are commonly deregulated in cancer, only a few studies have sought to comprehensively examine the spectrum of phosphorylation-mediated signaling downstream of each of these key signaling nodes. In this study, we completed a label-free quantitative analysis of oncogenic K-Ras, activated Cdc42, and PAK4-mediated phosphorylation signaling, and report relative quantitation of 2152 phosphorylated peptides on 1062 proteins. We define the overlap in phosphop...

  8. P-cadherin promotes collective cell migration via a Cdc42-mediated increase in mechanical forces

    Science.gov (United States)

    Plutoni, Cédric; Bazellieres, Elsa; Le Borgne-Rochet, Maïlys; Comunale, Franck; Brugues, Agusti; Séveno, Martial; Planchon, Damien; Thuault, Sylvie; Morin, Nathalie; Bodin, Stéphane; Trepat, Xavier

    2016-01-01

    Collective cell migration (CCM) is essential for organism development, wound healing, and metastatic transition, the primary cause of cancer-related death, and it involves cell–cell adhesion molecules of the cadherin family. Increased P-cadherin expression levels are correlated with tumor aggressiveness in carcinoma and aggressive sarcoma; however, how P-cadherin promotes tumor malignancy remains unknown. Here, using integrated cell biology and biophysical approaches, we determined that P-cadherin specifically induces polarization and CCM through an increase in the strength and anisotropy of mechanical forces. We show that this mechanical regulation is mediated by the P-cadherin/β-PIX/Cdc42 axis; P-cadherin specifically activates Cdc42 through β-PIX, which is specifically recruited at cell–cell contacts upon CCM. This mechanism of cell polarization and migration is absent in cells expressing E- or R-cadherin. Thus, we identify a specific role of P-cadherin through β-PIX–mediated Cdc42 activation in the regulation of cell polarity and force anisotropy that drives CCM. PMID:26783302

  9. NRP1 Regulates CDC42 Activation to Promote Filopodia Formation in Endothelial Tip Cells

    Directory of Open Access Journals (Sweden)

    Alessandro Fantin

    2015-06-01

    Full Text Available Sprouting blood vessels are led by filopodia-studded endothelial tip cells that respond to angiogenic signals. Mosaic lineage tracing previously revealed that NRP1 is essential for tip cell function, although its mechanistic role in tip cells remains poorly defined. Here, we show that NRP1 is dispensable for genetic tip cell identity. Instead, we find that NRP1 is essential to form the filopodial bursts that distinguish tip cells morphologically from neighboring stalk cells, because it enables the extracellular matrix (ECM-induced activation of CDC42, a key regulator of filopodia formation. Accordingly, NRP1 knockdown and pharmacological CDC42 inhibition similarly impaired filopodia formation in vitro and in developing zebrafish in vivo. During mouse retinal angiogenesis, CDC42 inhibition impaired tip cell and vascular network formation, causing defects that resembled those due to loss of ECM-induced, but not VEGF-induced, NRP1 signaling. We conclude that NRP1 enables ECM-induced filopodia formation for tip cell function during sprouting angiogenesis.

  10. Waist Circumferences of Chilean Students: Comparison of the CDC-2012 Standard and Proposed Percentile Curves

    Directory of Open Access Journals (Sweden)

    Rossana Gómez-Campos

    2015-07-01

    Full Text Available The measurement of waist circumference (WC is considered to be an important means to control overweight and obesity in children and adolescents. The objectives of the study were to (a compare the WC measurements of Chilean students with the international CDC-2012 standard and other international standards, and (b propose a specific measurement value for the WC of Chilean students based on age and sex. A total of 3892 students (6 to 18 years old were assessed. Weight, height, body mass index (BMI, and WC were measured. WC was compared with the CDC-2012 international standard. Percentiles were constructed based on the LMS method. Chilean males had a greater WC during infancy. Subsequently, in late adolescence, males showed values lower than those of the international standards. Chilean females demonstrated values similar to the standards until the age of 12. Subsequently, females showed lower values. The 85th and 95th percentiles were adopted as cutoff points for evaluating overweight and obesity based on age and sex. The WC of Chilean students differs from the CDC-2012 curves. The regional norms proposed are a means to identify children and adolescents with a high risk of suffering from overweight and obesity disorders.

  11. CDC-42 and RAC-1 regulate opposite chemotropisms in Neurospora crassa.

    Science.gov (United States)

    Lichius, Alexander; Goryachev, Andrew B; Fricker, Mark D; Obara, Boguslaw; Castro-Longoria, Ernestina; Read, Nick D

    2014-05-01

    Cell polarization and fusion are crucial developmental processes that occur in response to intracellular and extracellular signals. Asexual spores (conidia) of the mold Neurospora crassa differentiate two types of polarized cell protrusions, germ tubes and conidial anastomosis tubes (CATs), which exhibit negative and positive chemotropism, respectively. We provide the first evidence that shared and separate functions of the Rho-type GTPases CDC-42 and RAC-1 regulate these opposite chemotropisms. We demonstrate that RAC-1 is essential for CAT formation and cell fusion, whereas CDC-42 is necessary and sufficient for normal germ tube development. Cdc42-Rac-interactive-binding (CRIB) reporters were constructed to exclusively label locally activated GTP-bound GTPases. Time course analyses showed that repositioning of these activated GTPase clusters within germ tube and CAT tip apices controls directional growth in the absence of a tip-localized vesicle supply center (Spitzenkörper). We propose a model in which the local assembly of a plasma-membrane-associated GTPase-PAK-MAPK signaling platform regulates chemoattractant perception and secretion in order to synchronize oscillatory cell-cell communication and directional CAT tip growth. PMID:24790223

  12. Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): evidence for its role in regulating oogenesis.

    Science.gov (United States)

    Wang, Lan-Mei; Zuo, Di; Lv, Wei-Wei; Wang, Dan-Li; Liu, A-Jing; Zhao, Yunlong

    2013-02-25

    Cdc2 kinase is a catalytic subunit of the maturation-promoting factor (MPF), a central factor for inducing the meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, a complete cDNA sequence of Cdc2 kinase was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. The Cdc2 cDNA (1,769 bp) encodes for a 299 amino acid protein with a calculated molecular weight of 34.7 kDa. Quantitative real-time PCR demonstrated that Cdc2 mRNA was expressed mainly in the ovary tissue and the expression decreased as the ovaries developed. Immunohistochemistry analysis revealed that the Cdc2 protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cdc2 kinase may play an important role in the gametogenesis and gonad development in C. quadricarinatus. PMID:23266620

  13. Simpósio Ibero-Americano de História da Enfermagem: novas perspectivas da produção intelectual em história da enfermagem Coloquio Ibero-Americano de Historia de la Enfermería: nuevas perspectivas de la producción intelectual en historia de la enfermería Ibero-American Symposium on History of Nursing: new perspectives of intellectual production in the history of nursing

    OpenAIRE

    Almerinda Moreira; Fernando Porto; Genival Fernandes de Freitas; Paulo Fernando de Souza Campos

    2009-01-01

    A produção intelectual apresentada no I Simpósio Ibero-Americano de História da Enfermagem foi organizada a partir de três eixos temáticos: antropologia do cuidado, história das instituições de ensino, de assistência e agremiações de enfermagem e história de vida, totalizando 198 estudos. Mediante os resultados apresentados a produção mostrada no simpósio apontou para a tendência dos estudos em história da enfermagem, nos eixos temáticos, pela ordem de interesse dos pesquisadores em: história...

  14. Sistema educativo y mercado de trabajo 

    OpenAIRE

    López Peñate, Celedonio

    1999-01-01

    [ES] Estudio de las condiciones que propiciaron el salto cualitativo producido en el desarrollo del Sistema Educativo canario a finales de los setenta, y aproximación a una evaluación de dicho sistema.

  15. Contexto General de los Sistemas Agrarios

    Directory of Open Access Journals (Sweden)

    Vélez Escobar Norberto

    1989-12-01

    Full Text Available Esta ponencia introdujo y delimitó el campo de trabajo para el panel: Formas o Sistemas de Producción Agraria. En ella se presentó y discutió la definición del concepto de FORMA DE PRODUCCION e igualmente planteó la taxonomía más general o "agrupamientos" de los sistemas de producción agraria en Colombia. Se identificaron cinco grupos de formas, en proceso de estructuración, empresariales, campesinas, de economía natural y experimentales; y a continuación se caracterizó y explicó cada uno de los cinco agrupamientos. La ponencia terminó sustentando la validez del trabajo académico basado en el enfoque de sistemas y presentó la evolución reciente de esta forma de trabajo en la Universidad Nacional, Seccional Medellín.

  16. SCODA para el Desarrollo de Sistemas Multiagente

    Directory of Open Access Journals (Sweden)

    Jesús A. Román

    2011-12-01

    Full Text Available El planteamiento de implementar sistemas multiagente de forma que puedan reutilizarse con distintos objetivos, dota a los desarrolladores de una metodología más eficaz en sus fines. Este artículo presenta SCODA, una arquitectura basada en cinco principios: estandaridad, especialización, facilidad de implementación, reutilización y computación distribuida. SCODA pretende facilitar el desarrollo de sistemas multiagente, basándose en el concepto de pequeños grupos de agentes denominados Comunidades Inteligentes Especializadas (CIE. Las CIE cuentan con funcionalidades específicas que permiten llevar a cabo la implementación de sistemas multiagente de forma escalable, dentro del marco de SCODA, pudiendo ser reutilizadas en diversos desarrollos.

  17. Psiconeuroinmunología: Conexiones entre sistema nervioso y sistema inmune

    Directory of Open Access Journals (Sweden)

    García López, Luis Joaquín

    2008-09-01

    Full Text Available La psiconeuroinmunología es una nueva parcela de la ciencia que apenas lleva tres décadas, pero que en los últimos años ha sido objeto de una enorme atención por diversos grupos de investigación. El articulo pretende exponer brevemente cómo están organizados los sistemas inmune y neuroendocrino, para después presentar los hallazgos que hasta el momento intentan dar explicación de las relaciones entre ambos con el sistema nervioso, así como las influencias de unos sobre otros, más concretamente expresar la relación entre los tres sistemas responsables de la defensa del organismo y, más ampliamente, de su mantenimiento homeostático. Cuando se trata de amenazas, el sistema nervioso central tiene que decidir qué sistema se pone en marcha, el sistema de estrés como energía para el ataque o la huida (un animal que nos quiere atacar, el sistema inmune para neutralizar el antígeno (un virus que nos quiere atacar o ambos (la energía es necesaria también para el sistema inmune. Como parece ser que en muchas ocasiones la depresión aparece tras los esfuerzos infructuosos de puesta en marcha de estrategias de afrontamiento, también hemos repasado sucintamente algunas investigaciones que relacionan al sistema inmune con la depresión, así como con los trastornos del sueño. Se finaliza con las aportaciones que la psicología puede ofrecer a nivel terapéutico.

  18. Nuclear distribution and chromatin association of DNA polymerase α-primase is affected by TEV protease cleavage of Cdc23 (Mcm10 in fission yeast

    Directory of Open Access Journals (Sweden)

    Gregan Juraj

    2005-06-01

    Full Text Available Abstract Background Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease. Results Insertion of a TEV protease cleavage site into Cdc23 allows in vivo removal of the C-terminal 170 aa of the protein by TEV protease induction, resulting in an S phase arrest. This C-terminal fragment of Cdc23 is not retained in the nucleus after cleavage, showing that it lacks a nuclear localization signal and ability to bind to chromatin. Using an in situ chromatin binding procedure we have determined how the S phase chromatin association of DNA polymerase α-primase and the GINS (Sld5-Psf1-Psf2-Psf3 complex is affected by Cdc23 inactivation. The chromatin binding and sub-nuclear distribution of DNA primase catalytic subunit (Spp1 is affected by Cdc23 cleavage and also by inactivation of Cdc23 using a degron allele, implying that DNA polymerase α-primase function is dependent on Cdc23. In contrast to the effect on Spp1, the chromatin association of the Psf2 subunit of the GINS complex is not affected by Cdc23 inactivation. Conclusion An important function of Cdc23 in the elongation step of DNA replication may be to assist in the docking of DNA polymerase α-primase to chromatin.

  19. CDC25A Protein Stability Represents a Previously Unrecognized Target of HER2 Signaling in Human Breast Cancer: Implication for a Potential Clinical Relevance in Trastuzumab Treatment

    Directory of Open Access Journals (Sweden)

    Emanuela Brunetto

    2013-06-01

    Full Text Available The CDC25A-CDK2 pathway has been proposed as critical for the oncogenic action of human epidermal growth factor receptor 2 (HER2 in mammary epithelial cells. In particular, transgenic expression of CDC25A cooperates with HER2 in promoting mammary tumors, whereas CDC25A hemizygous loss attenuates the HER2-induced tumorigenesis penetrance. On the basis of this evidence of a synergism between HER2 and the cell cycle regulator CDC25A in a mouse model of mammary tumorigenesis, we investigated the role of CDC25A in human HER2-positive breast cancer and its possible implications in therapeutic response. HER2 status and CDC25A expression were assessed in 313 breast cancer patients and we found statistically significant correlation between HER2 and CDC25A (P = .007. Moreover, an HER2-positive breast cancer subgroup with high levels of CDC25A and very aggressive phenotype was identified (P = .005. Importantly, our in vitro studies on breast cancer cell lines showed that the HER2 inhibitor efficacy on cell growth and viability relied also on CDC25A expression and that such inhibition induces CDC25A down-regulation through phosphatidylinositol 3-kinase/protein kinase B pathway and DNA damage response activation. In line with this observation, we found a statistical significant association between CDC25A overexpression and trastuzumab-combined therapy response rate in two different HER2-positive cohorts of trastuzumab-treated patients in either metastatic or neoadjuvant setting (P = .018 for the metastatic cohort and P = .021 for the neoadjuvant cohort. Our findings highlight a link between HER2 and CDC25A that positively modulates HER2- targeted therapy response, suggesting that, in HER2-positive breast cancer patients, CDC25A overexpression affects trastuzumab sensitivity.

  20. El sistema linfático: el gran olvidado del sistema circulatorio.

    OpenAIRE

    Olmos Martínez, Susana; Gavidia Catalán, Valentín

    2014-01-01

    En el presente artículo se analizan los modelos mentales y las concepciones alternativas que tienen los estudiantes de distintos niveles educativos sobre el sistema linfático como componente del sistema circulatorio. Se establece una relación con las dificultades que se salvaron a lo largo de la historia en la adquisición de dichos conocimientos. Los resultados muestran una deficiente relación entre los sistemas linfáticos y sanguíneos, una falta de conocimientos tanto anatómicos como fisioló...

  1. Aplicação de sistemas operativos de tempo real no sistema Galileo

    OpenAIRE

    Guimarães, Ivo Daniel Oliveira

    2008-01-01

    Os sistemas operativos de tempo real têm sido imensamente utilizados no desenvolvimento de aplicações embebidas e/ou críticas. No entanto, todos apresentam problemas de fiabilidade e segurança na sua implementação ao disponibilizarem serviços e funcionalidades que ameaçam a integridade da aplicação e por vezes do próprio sistema operativo. Ao longo do documento serão analisados vários aspectos dos sistemas operativos de tempo real, passando por importantes especificações com...

  2. El Sistema Político Latinoamericano

    OpenAIRE

    Luis Dallanegra Pedraza

    2003-01-01

    Se ha escrito mucho sobre la problemática latinoamericana, y sobre sus aspectos históricos, especialmente en relación con Estados Unidos, y sobre las políticas que este país ha ido aplicando históricamente en sus sucesivos períodos de gobierno, hacia América Latina. Sin embargo, no se ha hecho un estudio profundo sobre el sistema político latinoamericano. Al menos sobre la existencia de un sistema político específico en América Latina que la caracterice desde ese punto de vista...

  3. Sistemas de Gestión Distribuidos

    OpenAIRE

    Pesado, Patricia; Bertone, Rodolfo Alfredo; Boracchia, Marcos; Thomas, Pablo Javier; Pasini, Ariel C.; Ricci, Guillermo; Labataglia, J.; De Giusti, Armando Eduardo

    2003-01-01

    Esta línea de I/D se refiere a realizar investigación y desarrollo en temas de Sistemas de Software Distribuido, particularmente los relacionados con los aspectos de Ingeniería de Software que se orientan al desarrollo e implementación de proyectos de sistemas distribuidos. Estas tareas abarcan aspectos propios de la arquitectura, comunicaciones, estructuración de bases de datos, así como especificación, validación y desarrollo de aplicaciones de software en ambientes distribuidos. Asimism...

  4. Sistema Expertos Probabilísticos

    OpenAIRE

    Gámez Martín, José Antonio; Puerta Callejón, José Miguel

    1998-01-01

    Un Sistema Experto es una herramienta informática que es capaz de simular el comportamiento de un experto humano en una materia especializada. Un problema clave en el desarrollo de sistemas expertos es encontrar la forma de representar y usar el conocimiento que los expertos humanos en esa materia poseen y utilizan. Este problema se hace más difícil por el hecho de que. En muchos campos, el conocimiento de los expertos es a menudo impreciso o incierto y. sin embargo, los expertos son capaces ...

  5. UVEDBA SISTEMA SPINETIX ZA ORGANIZIRANJE DOGODKOV

    OpenAIRE

    Šobot, Dragica

    2013-01-01

    V diplomskem delu je predstavljeno digitalno oglaševanje v kulturno-kongresni organizaciji Cankarjev dom z uporabo sistema SpinetiX. Cilj diplomskega dela je uporabiti ta sistem za hitro in učinkovito posredovanje sporočil ciljni publiki ter pokazati tehnične zmogljivosti sistema. V diplomskem delu so najprej podane osnove oglaševanja s poudarkom na digitalnem oglaševanju. Opisane so značilnosti konkretnega organizatorja kulturnih in kongresnih dogodkov. Sledi predstavitev glavnih komponent s...

  6. Ortogonalidad y capacidad en sistemas MIMO masivo

    OpenAIRE

    Romero Ibáñez, Irene

    2014-01-01

    La tecnología MIMO constituye un antes y un después en los sistemas de comunicaciones inalámbricas. Esta tecnología ofrece multitud de beneficios que nos acercan a cumplir con los desafíos impuestos por las limitaciones en un canal inalámbrico así como por las restricciones de nuestros recursos o el espacio físico ocupado por las antenas, entre otros. Además de explotar tanto la dimensión temporal como la frecuencial en sistemas inalámbricos convencionales con una única antena, las posibilida...

  7. El Derecho Administrativo del Sistema Comunitario Andino

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Santos Rodríguez

    2013-12-01

    Full Text Available Una de las tendencias contemporáneas del Derecho Administrativo es el reconocimiento de su existencia más allá de las fronteras de un Estado. Bajo esa premisa, se busca demostrar que en la Comunidad Andina de Naciones se configuran los elementos suficientes para afirmar que existe un Derecho Administrativo Comunitario Andino, en la medida en que de las normas andinas se desprende la existencia de una función administrativa andina, una organización administrativa al seno del Sistema Andino de Integración, un sistema de fuentes administrativas andina y un contencioso administrativo comunitario.

  8. CATEGORIZANDO A LOS USUARIOS DE SISTEMAS DIGITALES.

    OpenAIRE

    Denise Hernández y Hernández; Alberto Ramírez-Martinell; Daniel Cassany

    2014-01-01

    Repasamos las categorías más utilizadas para aludir a las características de las personas que utilizan algún tipo de sistema digital, ya sea por su grado de participación en la comu- nicación global y en los sistemas web -1.0 o 2.0-; por la actitud que toman ante el contenido digital -consumidores o productores-; por su intermitencia en el mundo digital -residentes o visitantes-; o por el momento histórico en el que nacieron -nativos digitales o inmigrantes digitales-, con el propósito de ref...

  9. Sistema de gestión documental

    OpenAIRE

    Fernández Gómez, Javier

    2015-01-01

    Esta memoria expone la planificación, el análisis, el diseño y el desarrollo de un sistema de gestión documental, en adelante SGD, configurable, es decir, adaptable a un ámbito determinado. El SGD consta de dos grupos de interfaces o funcionalidades claramente diferenciadas: administración y gestión documental. Aquesta memòria exposa la planificació, l'anàlisi, el disseny i el desenvolupament d'un sistema de gestió documental, d'ara endavant SGA, configurable, és a dir, adaptable a un àmbi...

  10. Sistemas de Indicadores de Desempenho em Projetos

    OpenAIRE

    Julia Garaldi Borges; Marly Monteiro de Carvalho

    2011-01-01

    O presente trabalho visa propor um sistema de indicadores de desempenho de projetos, a partir da análise da importância dos critérios de sucesso em projetos. A abordagem metodológica foi a de pesquisa-ação desenvolvida em uma empresa de engenharia, com atuação em diversos setores. Foram utilizadas várias fontes de evidência, tais como análise do atual sistema de indicadores da empresa, análise dos projetos nas bases de dados e entrevistas com representantes do escritório de projetos e com ger...

  11. Sistema de comunicaciones para una planta fotovoltaica

    OpenAIRE

    Martín Aragón, José Manuel

    2012-01-01

    Las plantas solares fotovoltaicas, que son cada vez más habituales en nuestra sociedad, necesitan contar con un sistema de comunicaciones que permita la monitorización continua del funcionamiento de los diferentes equipos así como el control remoto de los mismos y la regulación de la producción. En este Proyecto se ha estudiado la estructura eléctrica y constructiva de una planta fotovoltaica genérica, prestando especial atención a los requerimientos que debe reunir el sistema de comunicac...

  12. Sistema de realidad aumentada para aplicaciones Android

    OpenAIRE

    Fernández Sánchez, Natalia Mercedes

    2012-01-01

    El objetivo principal de este proyecto es la creación de un sistema de realidad aumentada para el sistema operativo Android con idea de que sea utilizado para la creación de un videojuego, pero que a su vez sea lo suficientemente versátil como para poder utilizarse en otro tipo de aplicaciones. De este objetivo principal podemos obtener los objetivos secundarios: - Conocer las características que ofrecen los teléfonos móviles y como éstas pueden ser aprovechadas para la crea...

  13. Estudo comparativo de sistemas em aquecimento

    OpenAIRE

    Castro, Inês Marques Costa Borges de

    2013-01-01

    O presente estudo pretende avaliar o desempenho energético e rentabilidade do uso de equipamento de climatização para o aquecimento ambiente de uma casa sénior, através de sistemas tradicionais de produção de água aquecida, como a caldeira e a tradicional bomba de calor, e um sistema apoiado num equipamento de produção solar térmico. Perante uma determinada arquitectura, foram calculados os caudais de ar novo, de forma a garantir a qualidade do ar interior, cumprindo os requisitos da actua...

  14. Estabilización robusta de sistemas lineales

    Directory of Open Access Journals (Sweden)

    Horacio Leyva Castellanos

    2013-12-01

    Full Text Available En este trabajo se muestra un conjunto de resultados que permiten abordar el problema de la estabilización robusta de una familia de sistemas lineales positivos. Se describen dos nuevas aplicaciones en la teoría de control positivo para sistemas compartimentales y se muestra la aplicación de un teorema de robustez para tales sistemas

  15. LA MIGRACIÓN EN EL SISTEMA INTERAMERICANO DE DERECHOS HUMANOS MIGRATION IN THE INTER-AMERICAN HUMAN RIGHTS SYSTEM

    Directory of Open Access Journals (Sweden)

    Verónica Torres-Marenco

    2011-01-01

    Full Text Available El Sistema Interamericano de Derechos Humanos, SIDH, establece unos derechos básicos reconocidos a los migrantes, sin que esto implique un derecho a la migración per se. Estos derechos se encuentran en el marco del principio de no discriminación y la obligación general de respeto de los derechos humanos reconocidos en los instrumentos internacionales. Sin embargo, no ha sido aplicado por los gobiernos nacionales. El desarrollo de sus políticas migratorias se ha dado en violación del derecho a la libertad, la nacionalidad, las garantías judiciales, el debido proceso, etc. Esta situación no parece mejorar en el futuro inmediato, razón por la cual el Sistema Interamericano de Derechos Humanos, SIDH, debe fortalecer su influencia en los estados miembros de la Organización de Estados Americanos, OEA, con el objetivo de discutir no solo el problema del reconocimiento de unos derechos básicos a los inmigrantes indocumentados, sino la pobreza frente al modelo de desarrollo neoliberal en América Latina que constituye una de las causas más frecuentes de la migración.The Inter-American Human Rights System has established a minimum of rights that should be recognized to every migrant. These rights are supported by the principle of non discrimination and the general obligation to respect every human right established in the international instruments. This does not require an assumption of the existence of a right to migration. However, those guarantees have not been yet applied by the governments. The current migration policies are in violation of the right to liberty, nationality, judicial guarantees, due process of law, etc. This situation does not seem to improve in the short-term. This is the reason why the InterAmerican Human Rights System, IHRS, should strengthen the influence on the inside of the members of the Organization of American States, OAS, with the purpose not only to discuss the recognition of the basic rights for undocumented

  16. Sistemas territoriales de salud frente al sistema general de seguridad social en salud de Colombia

    OpenAIRE

    Jahir Alexander Gutiérrez Ossa; Rubén Darío Restrepo Avendaño

    2014-01-01

    Introducción: El artículo expone los elementos y fundamentos que podrían avalar la generación de sistemas de seguridad social en salud territorial, en complementación con el sistema general preexistente. Materiales y Métodos: La eclosión generalizada en los sistemas de salud, implica la revisión de los modelos propuestos de carácter central y territorial. El análisis económico institucional, permite analizar las condiciones que tienen los sistemas generales para impactar en los esquemas terri...

  17. Isolation of a cdc28 mutation that abrogates the dependence of S phase on completion of M phase of the budding yeast cell cycle

    Indian Academy of Sciences (India)

    Santanu Kumar Ghosh; Pratima Sinha

    2000-01-01

    We have isolated a mutation in the budding yeast Saccharomyces cerevisisae CDC28 gene that allows cdc13 cells, carrying damaged DNA, to continue with the cell division cycle. While cdc13 mutant cells are arrested as large-budded cells at the nonpermissive temperature 37°C, the cdc13 cdc28 double mutant culture showed cells with one or more buds, most of which showed apical growth. The additional buds emerged without the intervening steps of nuclear division and cell separation. We suggest that the cdc28 mutation abrogates a checkpoint function and allows cells with damaged or incompletely replicated DNA an entry to another round of cell cycle and bypasses the mitotic phase of the cell cycle.

  18. Sistemas ultrasónicos para la irrigación del sistema de conductos radiculares

    Directory of Open Access Journals (Sweden)

    A. García Delgado

    2014-04-01

    Full Text Available La eliminación de los restos de tejido pulpar vital y necrótico y de los microorganismos del sistema de conductos radiculares, es esencial para el éxito en endodoncia. La desinfección del canal radicular mediante la irrigación e instrumentación es el factor más importante en la prevención y tratamiento de la periodontitis apical. Al ser imposible con la instrumentación llegar a todas las áreas del sistema de conductos, la irrigación cobra especial importancia. Por este motivo, en la última década se han desarrollado una serie de sistemas de dispensación y agitación de irrigantes, tales como los ultrasonidos. En concreto, en este artículo de revisión se evalúa la información disponible de los diez últimos años sobre la efectividad de los sistemas ultrasónicos para eliminar bacterias, tejido pulpar, restos de dentina y barrillo dentinario, la capacidad de estos dispositivos para hacer que el irrigante penetre en el sistema de conductos radiculares y la seguridad en su uso. Los resultados de la revisión muestran que los sistemas ultrasónicos son más eficaces en el desbridamiento químico, biológico y físico del sistema de conductos radiculares que los sistemas de irrigación convencional, a la vez que son seguros.

  19. Elementos del sistema electoral del Congreso Mexicano

    Directory of Open Access Journals (Sweden)

    Aldo Medina García

    2013-08-01

    Full Text Available El presente artículo es un estudio de los componentes del sistema electoral, diseñado por el legislador mexicano para integrar al parlamento federal. Estudio desarrollado a sabiendas de la importancia que reviste el sistema electoral como un medio que permite a los ciudadanos convertir su voto expresado en las urnas en escaños a favor de determinado candidato o partido. En dicho análisis tomamos en cuenta la evolución constitucional del sistema electoral mexicano, la participación de las fuerzas minoritarias en la integración del Senado y de la Cámara de Diputados, la división que se hace del territorio en circunscripciones electorales, la barrera electoral que se fija a los partidos políticos para acceder a la representación en el Parlamento, la fórmula de asignación que se aplica a la votación obtenida, para finalmente concluir que el sistema electoral mexicano es esencialmente mayoritario aun cuando se vista de proporcional.

  20. Sistema de Reinscripciones Vía Internet

    Directory of Open Access Journals (Sweden)

    Bertha Alicia Góngora Serna

    2005-01-01

    Full Text Available Este Proyecto se enfoca al diseño de una página Web para optimizar el sistema de reinscripciones del Instituto Tecnológico de Aguascalientes (ITA, la cual permite que los alumnos utilicen menos tiempo en el proceso de inscripción, además de facilitar que dicha actividad se pueda realizar desde su hogar. Para la elaboración de este proyecto se requirió de Tecnologías web como HTML, JavaScript y JSP. La información necesaria fue reutilizada de los datos que actualmente genera el sistema de reinscripciones. La investigación que se llevó a cabo para sustentar este proyecto indaga desde la historia del Internet, una explicación breve de las tecnologías Web requeridas, algunos sistemas de reinscripciones de diferentes escuelas de nivel superior del país, así como el funcionamiento básico del sistema actual de reinscripciones del ITA