WorldWideScience

Sample records for cdc americano sistema

  1. Potenciais benefícios do sistema de rastreabilidade animal dos EUA para o setor de carnes americano

    OpenAIRE

    Resende Filho, Moisés de Andrade

    2008-01-01

    Este artigo investigou os potenciais ganhos do setor de carnes americano advindos da implantação do Sistema Nacional de Identificação Animal (NAIS), dos EUA. Foram analisados os potenciais efeitos do NAIS sobre a percepção de risco dos consumidores americanos em relação aos perigos decorrentes do consumo das carnes bovina, suína e de aves e seus derivados. Sistemas de equações de demanda foram estimados, incorporando-se como "proxies" da percepção de risco do consumidor, séries de índices de ...

  2. Potenciais benefícios do sistema de rastreabilidade animal dos EUA para o setor de carnes americano

    Directory of Open Access Journals (Sweden)

    Moisés de Andrade Resende Filho

    2008-12-01

    Full Text Available Este artigo investigou os potenciais ganhos do setor de carnes americano advindos da implantação do Sistema Nacional de Identificação Animal (NAIS, dos EUA. Foram analisados os potenciais efeitos do NAIS sobre a percepção de risco dos consumidores americanos em relação aos perigos decorrentes do consumo das carnes bovina, suína e de aves e seus derivados. Sistemas de equações de demanda foram estimados, incorporando-se como "proxies" da percepção de risco do consumidor, séries de índices de segurança do alimento, separadamente construídas para cada tipo de carne. Tais séries foram concebidas, somando-se o número de referências nos principais jornais americanos a problemas de segurança da carne. Foi utilizado o melhor modelo estimado, escolhido com base em uma série de testes de especificação, para se construir três cenários, simulando-se os casos em que o NAIS não está implementado; que está implementado apenas para o gado bovino; e que está implementado para suínos e bovinos. Foram utilizadas as diferenças entre as receitas estimadas para cada cenário e para cada tipo de carne, como uma medida do potencial ganho advindo da implementação do NAIS. Foi concluído que os setores da carne bovina e suína poderiam arcar com os custos do NAIS. Esse resultado, contudo, depende de o quanto desses potenciais ganhos chegarão efetivamente aos produtores agrícolas.This article investigates the potential gains to the U.S. meat sector with the implantation of the U.S. National Animal Identification System (NAIS. The focus is on the effect that the NAIS could have on consumers' risk perception about eating meat. System of demand equations are estimated using time series of food safety indexes variables used as proxies for consumers' reactions to news on meat safety issues. The series of food safety indexes are built on the basis of the number of food safety news reported in top U.S. newspapers. Using the preferred model

  3. Políticas e Sistemas de Saúde Ibero-americanos - uma análise comparada: Entrevista de Eleonor Minho Conill.

    Directory of Open Access Journals (Sweden)

    Tamires Marinho dos SANTOS

    2016-05-01

    Full Text Available Entrevista - Eleonor Minho Conill é graduada em Medicina pela Universidade Federal do Rio Grande do Sul, Mestre em Saúde Comunitária pela Université de Montréal e Doutora em Desenvolvimento Econômico e Social pela Université Paris I (Pantheon -Sorbonne. Atuou como docente e pesquisadora nas Universidades Federais do Rio Grande do Sul, Rio de Janeiro, Santa Catarina e na Escola Nacional de Saúde Pública da Fundação Oswaldo Cruz. Trabalha na área de políticas, planejamento, organização, avaliação e análise comparada de sistemas de saúde. Foi consultora de pesquisa na Escola de Governo da FIOCRUZ e na Red de Investigaciónen Sistemas y Servicios de SaluddelConoSur. Integra a Comissão de Política Editorial da Revista Ciência e Saúde Coletiva da ABRASCO e, é membro do corpo editorial da Revista Saúde em Debate do CEBES. Colaborou na fundação do Observatório Ibero-Americano de Políticas e Sistemas de Saúde (OIAPSS coordenando o projeto para desenvolvimento da matriz analítica deste Observatório.

  4. Ukola Club. Bar americano

    Directory of Open Access Journals (Sweden)

    Azpiazu, J. R.

    1961-03-01

    Full Text Available En la calle de Serrano, aprovechando un semisótano dedicado a otro negocio anteriormente, se ha instalado un bar americano, de cuyo interior ofrecemos algunos pormenores. Se han cuidado, especialmente, las condiciones acústicas, resueltas por medio de un techo de escayola perforada, con vitrofib en su parte superior, y paredes de madera, que contribuyen a darle un ambiente cálido y acogedor. El soporte de hierro laminado existente en el centro del local, cuya supresión hubiera sido costosa, se ha revestido con lajas de mármol que le convierten en un elemento decorativo.

  5. El escritor americano

    Directory of Open Access Journals (Sweden)

    Cristina Iglesia

    2006-04-01

    Full Text Available Facundo se escribe para los no saben leer América. Este es uno de los puntos de partida de Sarmiento : hay una mala lectura europea de América, sobre todo de la Argentina, del Río de la Plata, de esta parte de América que es, en realidad, la única que a Sarmiento le interesa. “Sus más hábiles políticos no han alcanzado a comprender nada de lo que sus ojos han visto al echar una mirada precipitada sobre el poder americano que desafiaba a la gran nación” escribe en Viajes.Y Europa no sabe leer ...

  6. sistemas

    Directory of Open Access Journals (Sweden)

    Diego Alejandro Muñoz Gaviria

    2006-01-01

    Full Text Available El texto presentado a continuación, plantea en términos generales las principales posturas conceptuales del sociólogo alemán Niklas Luhmann sobre las ideas de Max Weber y su teoría de la acción social, y de Talcott Parsons y sus planteamientos en torno a la teoría de los sistemas sociales. Al final del escrito se hace especial énfasis en el rol protagónico que para Luhmann tienen las ciencias sociales y humanas en la autorreferencia del sistema social.

  7. Argos Americano: incertidumbre e independencia

    Directory of Open Access Journals (Sweden)

    Julián Penagos Carreño

    2015-01-01

    Full Text Available El presente artículo es el resultado del análisis léxico métrico al periódico cartagenero el Argos Americano. Papel político, económico y literario de Cartagena de Indias (Septiembre de 1810 -Marzo de 1812 , que muestra las distintas discusiones que se dieron en torno a temas como nación, gobierno e independencia durante una época turbulenta cuando España había sido invadida por los ejércitos de Napoleón, y el rey Fernando VII fue hecho prisionero, lo cual dejó un vacío de poder que sumió a las provincias del continente americano en la incertidumbre por definir un gobierno. Este trabajo hace parte del proyecto de investigación Historia Cultural de la Prensa en la Nueva Granada ( 1810-1820 , patrocinado por la Dirección de Investigaciones y la Facultad de Comunicación de la Universidad de La Sabana.

  8. Pela democracia dos sistemas de avaliação da produção acadêmica: convergências de estudiosos latino-americanos e europeus

    Directory of Open Access Journals (Sweden)

    Aimée Vega Montiel

    2013-01-01

    Full Text Available El presente texto es la transcripción de una conferencia pronunciada en 2011, en el encuentro anual de la Europeean Comunication Research and Education Association (ECREA, que plantea la discusión sobre los efectos de la supremacía de los investigadores del norte sobre los investigadores del sur en el contexto de la evaluación de la calidad de la producción académica. Aunque se reconozcan las especificidades locales y regionales, así como la necesidad de adopción de diferentes metodologías e instrumentos de investigación para el estudio de problemas distintos, en contextos sociales distintos, la producción académica de los estudiosos latinoamericanos sigue siendo sometida a patrones definidos en otras latitudes del mundo. Se argumenta que la supremacía científica de los investigadores del norte se debe, fundamentalmente, a la supremacía del inglés en el sistema académico internacional, que limita las posibilidades de diseminación del conocimiento en otras lenguas.

  9. ATLETAS DE TAEKWONDO TÊM MELHOR CONTROLE POSTURAL DO QUE ATLETAS DE HANDEBOL E FUTEBOL AMERICANO

    OpenAIRE

    Shirabe, Nelson Akio; Silva, Rubens Alexandre da; Oliveira, Márcio Rogério; Nowotny, Alexandre Henrique; Sturion, Leandro Amaral; Gil, André Wilson de Oliveira; Andraus, Rodrigo Antonio Carvalho; Carvalho, Adriana Paula Fontana

    2017-01-01

    RESUMO Introdução: O controle postural é um pré-requisito importante para o desempenho do atleta no esporte. Além disso, o sistema de controle postural contribui para a prevenção de lesões. Déficits nesse sistema podem levar a instabilidade corporal e sobrecarga das estruturas musculoesqueléticas, gerando disfunção e dor. Objetivos: A proposta deste estudo foi avaliar o controle postural em três diferentes modalidades esportivas: taekwondo, handebol e futebol americano. Métodos: Todos os atle...

  10. Pensamento, Teoria e Estudos Latino-americanos

    Directory of Open Access Journals (Sweden)

    Jacques de Novion

    2014-12-01

    Full Text Available RESUMO O presente trabalho configura como artigo inicial do Dossiê Especial – Pensamento, Teoria e Estudos Latino-americanos, organizado conjuntamente por nós, a pedido da Revista de Estudos e Pesquisas sobre as Américas (CEPPAC, do Instituto de Ciências Sociais (ICS, da Universidade de Brasília (UnB. De forma breve, este artigo apresenta a importância alcançada pelo Pensamento, pela Teoria, e, sobre tudo, pelos Estudos Latino-americanos nas últimas décadas. Em seguida, o artigo apresenta os quatorze trabalhos e uma resenha que compõem este Dossiê, resultado da contribuição de diferentes pesquisadores de distintas localidades da região, organizados em três blocos: Ciências Sociais Latino-americanas, Pensamento e Estudos. PALAVRAS CHAVE: Ciências Sociais Latino-americanas; Pensamento Latino-americanos; Teoria Latino-americana; Estudos Latino-americanos. --- RESUMEN El presente trabajo configura como articulo inicial del Dossier Especial – Pensamiento Teoría y Estudios Latinoamericanos, organizado conjuntamente por nosotros, a pedido de la Revista de Estudos e Pesquisas sobre as Américas (CEPPAC, del Instituto de Ciências Sociais (ICS, de la Universidade de Brasília (UnB. De forma breve, este articulo presenta la importancia alcanzada por el Pensamiento, por la Teoría, y, sobre todo, por los Estudios Latinoamericanos en las últimas décadas. En seguida, el articulo presenta los catorce trabajos e una reseña que componen este Dossier, resultado de la contribución de diferentes investigadores de distintas localidades de la región, organizados en tres bloques: Ciencias Sociales Latinoamericanas, Pensamiento y Estudios. PALABRAS CLAVE: Ciencias Sociales Latinoamericanas; Pensamiento Latinoamericano; Teoría Latinoamericana; Estudios Latinoamericanos. --- ABSTRACT This paper is set up as the initial article of this Special Dossier - Thought, Theory and Latin American Studies, which we organized collectively, at the request

  11. CDC Disease Detective Camp

    Centers for Disease Control (CDC) Podcasts

    2010-08-02

    The CDC Disease Detective Camp gives rising high school juniors and seniors exposure to key aspects of the CDC, including basic epidemiology, infectious and chronic disease tracking, public health law, and outbreak investigations. The camp also helps students explore careers in public health.  Created: 8/2/2010 by Centers for Disease Control and Prevention (CDC).   Date Released: 8/2/2010.

  12. CDC Child Growth Charts

    Data.gov (United States)

    U.S. Department of Health & Human Services — CDC child growth charts consist of a series of percentile curves that illustrate the distribution of selected body measurements in U.S. children. Pediatric growth...

  13. CDC WONDER: Births

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Births (Natality) online databases in CDC WONDER report birth rates, fertility rates and counts of live births occurring within the United States to U.S....

  14. ATLETAS DE TAEKWONDO TÊM MELHOR CONTROLE POSTURAL DO QUE ATLETAS DE HANDEBOL E FUTEBOL AMERICANO

    Directory of Open Access Journals (Sweden)

    Nelson Akio Shirabe

    Full Text Available RESUMO Introdução: O controle postural é um pré-requisito importante para o desempenho do atleta no esporte. Além disso, o sistema de controle postural contribui para a prevenção de lesões. Déficits nesse sistema podem levar a instabilidade corporal e sobrecarga das estruturas musculoesqueléticas, gerando disfunção e dor. Objetivos: A proposta deste estudo foi avaliar o controle postural em três diferentes modalidades esportivas: taekwondo, handebol e futebol americano. Métodos: Todos os atletas executaram o teste de equilíbrio unipodal sobre uma plataforma de força nos membros inferiores direito e esquerdo. Foram realizados três testes de trinta segundos cada, com os olhos abertos. A média dos três testes foi utilizada para mensurar os parâmetros de controle postural, centro de pressão e velocidade dos deslocamentos anteroposteriores e mediolaterais. Resultados: Diferenças significativas foram observadas entre os três grupos em todos os parâmetros analisados (P = <0,04. A análise post hoc revelou que os atletas de taekwondo tiveram melhor controle postural (P = < 0,035 dos membros inferiores em comparação com as outras duas modalidades. Não foram observadas diferenças significativas entre o handebol e o futebol americano. Conclusão: Os atletas do taekwondo têm melhor controle postural durante o teste de equilíbrio unipodal do que os atletas do handebol e do futebol americano.

  15. Dos americanos hablan de España

    Directory of Open Access Journals (Sweden)

    Helcías Martán Góngora

    1965-09-01

    Full Text Available El tema universal de España vuelve a resonar en la voz de los poetas americanos. El motivo noble se renueva en la generación que regresa a la península descubridora, a trueque de las negaciones, en la carabela de sus estrofas.

  16. CDC 7600 Module

    CERN Multimedia

    1970-01-01

    The CDC 7600 has been created by Seymour Cray. It was designed to be compatible with the 6600, which allows for a substantial increase in performance. Furthermore the rise of new technologies has enabled this performance by reducing the minor cycle clock period from 100 ns to 27.5 ns (4 time faster). A very large machine, the 7600 had over 120 miles of hand-wired interconnections. It was the most powerful computer of its time. However, this speed caused a ground-loop problem causing intermittent faults, and eventually requiring all modules to be fitted with sheathed rubber bands. The CDC 7600 was replaced in 1983 by CRAY-1A.

  17. Hispanic Health: CDC Vitalsigns

    Science.gov (United States)

    ... Injury Prevention & Control Gateway to Health Communication & Social Marketing Practice On Other Web Sites MedlinePlus – Hispanic American ... MB] en Español [PDF – 1.61 MB] CDC Digital Press Kit Read the MMWR Science Clips Language: ...

  18. CDC 6600 Cordwood Module

    CERN Multimedia

    1964-01-01

    The CDC 6600 cordwood module containing 64 silicon transistors. The module was mounted between two plates that were cooled conductive by a refrigeration unit via the front panel. The construction of this module uses the cord method, so called because the resistors seem to be stacked like cord between the two circuit boards in order to obtain a high density. The 6600 model contained nearly 6,000 such modules.

  19. CDC Lab Values

    Centers for Disease Control (CDC) Podcasts

    2015-02-02

    More than fifteen hundred scientists fill the lab benches at CDC, logging more than four million hours each year. CDC’s laboratories play a critical role in the agency’s ability to find, stop, and prevent disease outbreaks. This podcast provides a brief overview of what goes on inside CDC’s labs, and why this work makes a difference in American’s health.  Created: 2/2/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 2/2/2015.

  20. CDC Vital Signs: Legionnaires' Disease

    Science.gov (United States)

    ... preventable with more effective water management. Problem Water management problems can lead to Legionnaires’ disease outbreaks. What ... Privacy FOIA No Fear Act OIG 1600 Clifton Road Atlanta , GA 30329-4027 USA 800-CDC-INFO ( ...

  1. CDC WONDER: Mortality - Infant Deaths

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Infant Deaths (from Linked Birth / Infant Death Records) online databases on CDC WONDER provide counts and rates for deaths of children under 1 year...

  2. CDC PRAMStat Data for 2011

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  3. CDC PRAMStat Data for 2010

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  4. CDC PRAMStat Data for 2002

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  5. CDC PRAMStat Data for 2006

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  6. CDC PRAMStat Data for 2004

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  7. CDC PRAMStat Data for 2007

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  8. CDC PRAMStat Data for 2000

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  9. CDC PRAMStat Data for 2005

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  10. CDC PRAMStat Data for 2009

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  11. CDC PRAMStat Data for 2008

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  12. CDC PRAMStat Data for 2003

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  13. CDC PRAMStat Data for 2001

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  14. CDC Vital Signs: Preventing Melanoma

    Science.gov (United States)

    ... not use the device. Include warning statements in marketing materials about the risk of using the device. ... MB] en Español [PDF – 1.16 MB] CDC Digital Press Kit Read the MMWR Science Clips Language: ...

  15. Computer Center CDC Reference Manual.

    Science.gov (United States)

    1984-09-01

    34 Introduction *"- 1. For card decks, the following job card requirements apply at the DTNSRDC site: MFF - CDC CYBER 176 - orange (Carderock) yellow ...stripe (Annapolis) * MFE - CDC CYBER 750 - blue or purple (Carderock) yellow stripe (Annapolis) * No other cards in the deck should be any of the above...semi-private files day -OFF -Turn off dayfile messages for individual MSAUDITs for LO=FP (could generate wallpaper ). Has no effect on other options

  16. Aproximaciones a una estética de lo americano

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    María del Milagro Casalla

    2010-01-01

    Full Text Available El legado filosófico que nos ha dejado Rodolfo Kusch tiene cada vez más presencia y valor en este largo itinerario de descubrirnos como americanos. La pasión de este pensador por la América profunda es una fuente de ins- piración para quienes creemos que vivir en América no es simplemente un episodio geográfico sino un horizonte desde y a partir del cual construimos nuestra identidad. En este trabajo se pretende recrear sus consideracio - nes estéticas recogidas en su ensayo “Anotaciones para una estética de lo americano”, escrito en el año 1955. Aunque se trata de uno de los primeros escritos de Rodolfo Kusch, en el mismo se encuentran presentes numerosos conceptos que luego serán tematizados extensamente a lo largo de toda su obra. Considero que el abordaje a sus consideraciones estéticas no ha sido tan frecuentado como sí otros aspectos de su pensamiento (ser-estar, geocultura, símbolo y religión, etc.. Por ello, creo que este trabajo puede constituir una orientación preeliminar para futuras interpretaciones y, a la vez, ser un humilde homenaje a su filosofía, que, a lo largo de los años, sigue estimulando a antiguas y nuevas generaciones de pensadores dedicados a la filosofía latinoamericana.

  17. Stalking SARS: CDC at Work

    Centers for Disease Control (CDC) Podcasts

    2014-05-22

    In this podcast for kids, the Kidtastics talk about the SARS outbreak and how CDC worked to solve the mystery.  Created: 5/22/2014 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 5/22/2014.

  18. CDC Vital Signs: Hispanic Health

    Science.gov (United States)

    ... Injury Prevention & Control Gateway to Health Communication & Social Marketing Practice On Other Web Sites MedlinePlus – Hispanic American ... MB] en Español [PDF – 1.61 MB] CDC Digital Press Kit Read the MMWR Science Clips Language: ...

  19. Presencia de visón americano (Neovison vison, Schreber 1777) en Chiloé, Chile: ¿inicio de una invasión biológica?

    OpenAIRE

    G. Vergara; J. Valenzuela

    2015-01-01

    El visón americano fue introducido en Chile en la década de 1930 y actualmente presenta una amplia distribución geográfica. Sin embargo, no se han reportado poblaciones establecidas en muchos sistemas insulares. El día 6 de febrero de 2013, fue hallado un individuo juvenil en Chacao, Chiloé, Chile. El Canal de Chacao representa la única barrera biogeográfica a la isla de Chiloé, con una distancia mínima al continente de 2 km. Se presume que el individuo sorteó esta barrera por sus propios med...

  20. Um acontecimento, três jornais: processos de produção no Jornalismo latino-americano

    Directory of Open Access Journals (Sweden)

    Angela Zamin

    Full Text Available Nucleado por um acontecimento, o artigo tem por objetivo analisar os processos de produção que dão conta de sua materialidade jornalística. Trata-se do Angostura, crise colombo-equatoriana resultante do ataque do Exército colombiano a um acampamento das Farc localizado no Equador, em 1º de março de 2008. Com base no arcabouço dos estudos do acontecimento jornalístico, constatam-se fluxos do acontecimento e do Jornalismo (no espaço, entre editorias e sistemas informativos. Considera um conjunto de notícias sobre o acontecimento dos jornais latino-americanos O Estado de S.Paulo, El Tiempo e El Comercio, publicadas em 2008 e 2009. Por meio de uma análise da materialidade discursiva (FOUCAULT, 2005; GUILHAUMOU, 2002, identifica em que medida o acontecimento desestabiliza a ordem dos jornais e como, ao fazer trabalhar o acontecimento, o Jornalismo também se organiza.

  1. A dinâmica do novo regime monetário-financeiro norte-americano: uma hipótese de interpretação

    Directory of Open Access Journals (Sweden)

    Marcos Antonio Macedo Cintra

    2000-08-01

    Full Text Available No artigo é apresentada uma hipótese de interpretação sobre a dinâmica atual do sistema monetário e financeiro norte-americano, suas relações com as empresas, com o Federal Reserve, com o Tesouro e com o resto do mundo. Procurou-se mostrar a emergência de um modo de funcionamento específico do capitalismo predominantemente financeiro e rentista, em que prevalecem os mercados de capitais sofisticados e abrangentes, destinados a uma avaliação diária da massa de ativos (cambial, acionário, de títulos e imobiliário. Ademais, essa forma de gestão da riqueza se impôs ao resto do mundo como paradigma: as finanças passaram a operar num espaço mundial, hierarquizado a partir do sistema financeiro norte-americano e viabilizado pela política monetária do Estado hegemônico, imitada pelos demais países industrializados e em desenvolvimento.The article is an interpretation of the current dynamics of the North American monetary system, and its relations with enterprises, the Federal Reserve system, the Treasury and the rest of the world. The author looked into the emergence of a specific mode of operation of a predominantly financial, unearned income-based type of capitalism, in which sophisticated, encompassing capital markets, forced to perform a daily evaluation of assets (foreign exchange, shares, securities, and property prevail. Moreover, this way of managing resources has become a paradigm for the rest of the world: financial operations started to occur in a global space, stemming hierarchically from the North American financial system, and being made possible by the monetary policies of the hegemonic nation, and imitated by developed and developing countries.

  2. Repertorio Americano 1974-1982. Edición de ABRIL-MAYO-JUNIO-1981

    OpenAIRE

    Instituto de Estudios Latinoamericanos, UNA

    1981-01-01

    SA, Mano a mano con Jorge Luis Borges, 1* SA, Fotografía, 1* SA, Repertorio Americano, 2* SA, Jorge Luis Borges, Roberto Gritta y Jorge Smerling, 3* Carlos Enrique Aguirre Gómez, Poesía y circunstancialidad: una aproximación a Días y territorios de Isaac Felipe Azofeifa, 5* Edwin Salas Zamora, La perspectiva y otros aspectos de Gentes y gentecillas de Carlos Luis Fallas, 11* SA, Repertorio Americano. Colaboraciones, 13* Carlos Cortínez, Neruda y Lorca frente al amanecer, 14* Gilberto Tiviños,...

  3. Análisis, diseño, e implementación de un sistema informático de laboratorio clínico para el Centro de Diagnóstico Clínico C.D.C.

    OpenAIRE

    Paredes Galarza, Luis Vicente; Vargas Jácome, Silvana Lizette

    2011-01-01

    Describen brevemente las herramientas utilizadas en el desarrollo del sistema, haciendo una breve descripción de las ventajas y desventajas del uso de las mismas. Se muestra cómo se desarrolló la construcción del sistema CDCLab, tanto a nivel de la base de datos como de la solución y las iteraciones realizadas en el proyecto. Se realiza una explicación del código fuente más sobresaliente del proyecto, sobre la conexión con la base de datos, el registro del sistema y transacciones. También, se...

  4. CDC20 maintains tumor initiating cells

    Science.gov (United States)

    Xie, Qi; Wu, Qiulian; Mack, Stephen C.; Yang, Kailin; Kim, Leo; Hubert, Christopher G.; Flavahan, William A.; Chu, Chengwei; Bao, Shideng; Rich, Jeremy N.

    2015-01-01

    Glioblastoma is the most prevalent and lethal primary intrinsic brain tumor. Glioblastoma displays hierarchical arrangement with a population of self-renewing and tumorigenic glioma tumor initiating cells (TICs), or cancer stem cells. While non-neoplastic neural stem cells are generally quiescent, glioblastoma TICs are often proliferative with mitotic control offering a potential point of fragility. Here, we interrogate the role of cell-division cycle protein 20 (CDC20), an essential activator of anaphase-promoting complex (APC) E3 ubiquitination ligase, in the maintenance of TICs. By chromatin analysis and immunoblotting, CDC20 was preferentially expressed in TICs relative to matched non-TICs. Targeting CDC20 expression by RNA interference attenuated TIC proliferation, self-renewal and in vivo tumor growth. CDC20 disruption mediated its effects through induction of apoptosis and inhibition of cell cycle progression. CDC20 maintains TICs through degradation of p21CIP1/WAF1, a critical negative regulator of TICs. Inhibiting CDC20 stabilized p21CIP1/WAF1, resulting in repression of several genes critical to tumor growth and survival, including CDC25C, c-Myc and Survivin. Transcriptional control of CDC20 is mediated by FOXM1, a central transcription factor in TICs. These results suggest CDC20 is a critical regulator of TIC proliferation and survival, linking two key TIC nodes – FOXM1 and p21CIP1/WAF1 — elucidating a potential point for therapeutic intervention. PMID:25938542

  5. Engineering and public health at CDC.

    Science.gov (United States)

    Earnest, G Scott; Reed, Laurence D; Conover, D; Estill, C; Gjessing, C; Gressel, M; Hall, R; Hudock, S; Hudson, H; Kardous, C; Sheehy, J; Topmiller, J; Trout, D; Woebkenberg, M; Amendola, A; Hsiao, H; Keane, P; Weissman, D; Finfinger, G; Tadolini, S; Thimons, E; Cullen, E; Jenkins, M; McKibbin, R; Conway, G; Husberg, B; Lincoln, J; Rodenbeck, S; Lantagne, D; Cardarelli, J

    2006-12-22

    Engineering is the application of scientific and technical knowledge to solve human problems. Using imagination, judgment, and reasoning to apply science, technology, mathematics, and practical experience, engineers develop the design, production, and operation of useful objects or processes. During the 1940s, engineers dominated the ranks of CDC scientists. In fact, the first CDC director, Assistant Surgeon General Mark Hollis, was an engineer. CDC engineers were involved in malaria control through the elimination of standing water. Eventually the CDC mission expanded to include prevention and control of dengue, typhus, and other communicable diseases. The development of chlorination, water filtration, and sewage treatment were crucial to preventing waterborne illness. Beginning in the 1950s, CDC engineers began their work to improve public health while developing the fields of environmental health, industrial hygiene, and control of air pollution. Engineering disciplines represented at CDC today include biomedical, civil, chemical, electrical, industrial, mechanical, mining, and safety engineering. Most CDC engineers are located in the National Institute for Occupational Safety and Health (NIOSH) and the Agency for Toxic Substances and Disease Registry (ATSDR). Engineering research at CDC has a broad stakeholder base. With the cooperation of industry, labor, trade associations, and other stakeholders and partners, current work includes studies of air contaminants, mining, safety, physical agents, ergonomics, and environmental hazards. Engineering solutions remain a cornerstone of the traditional "hierarchy of controls" approach to reducing public health hazards.

  6. CDC's Response to Zika: Enjoy Your Vacation

    Science.gov (United States)

    ... your destination has Zika, check the CDC Travelers’ Health site for current travel notices: cdc. gov/ travel Pack to prevent • Insect repellent (Look for these ingredients: DEET, picaridin, IR3535, oil of lemon eucalyptus or para-menthane-diol, or 2-undecanone ) • ...

  7. The chipped eye: notes for a reading of BazarAmericano

    Directory of Open Access Journals (Sweden)

    Diego Poggiese

    2016-03-01

    Full Text Available BazarAmericano is the web magazine that stems from Punto de vista. Ever since its announced appearance in 2001, they both coexisted in their complementary heterogeneity until the last issue of the printed magazine in 2008, and since then it continues its own path. The challenge of this work lies in developing the reading of BazarAmericano, the object of our interest, in such a way that that mentioned complex relationship of complementarity and heterogeneity could be read, but also that the web magazine may be perceived in the connection that is claimed by a present tensed by the precedent of one of the most important magazines of the end of the century in Argentina, by the Argentinian history itself, by a state of the culture whose definition is under discussion and by the creative requirements of a technological foundation whose dynamics is constantly in ongoing process.

  8. Assessment of Cellular Mutagenicity of Americano Coffees from Popular Coffee Chains.

    Science.gov (United States)

    Liu, Zhen-Shu; Chen, Po-Wen; Wang, Jung-Yu; Kuo, Tai-Chen

    2017-09-01

    Coffee is a popular beverage worldwide, but coffee beans can be contaminated with carcinogens. The Ames Salmonella mutagenicity test is often used for analysis of carcinogens for mutagenicity. However, previous studies have provided controversial data about the direct mutagenicity of coffee beans based on Ames test results. This study was conducted to determine the mutagenicity of popular Americano coffee based on results from the Ames test. Coffee samples without additives that were served by five international coffee chain restaurants were subjected to the analysis using Salmonella Typhimurium tester strains TA98, TA100, and TA1535. The levels of bacterial revertants in samples from coffee chains were lower than the twofold criterion of the control sets, and no significant dose-response effect was observed with or without rat liver enzyme activation. These data indicate that Americano coffees from the selected coffee chains possessed no direct mutagenic activity with or without enzyme activation. These findings suggest a low mutagenic risk from Americano coffees served by the selected coffee chains and support the use of other methods to confirm the nonmutagenicity of coffee products. These results are consistent with most recent epidemiological reports.

  9. CDC STATE System Tobacco Legislation - Advertising

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2015. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Advertising. The STATE...

  10. CDC STATE System Tobacco Legislation - Preemption Summary

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Preemption. The STATE...

  11. CDC STATE System Tobacco Legislation - Smokefree Campus

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Campuses. The...

  12. CDC STATE System Tobacco Legislation - Preemption

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Preemption. The STATE...

  13. CDC STATE System Tobacco Legislation - Licensure

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Licensure. The STATE...

  14. CDC Wonder Vaccine Adverse Event Reporting System

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Vaccine Adverse Event Reporting System (VAERS) online database on CDC WONDER provides counts and percentages of adverse event case reports after vaccination,...

  15. CDC STATE System Tobacco Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Youth Access. The STATE...

  16. CDC STATE System Tobacco Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Youth Access. The STATE...

  17. CDC STATE System Tobacco Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Youth Access. The STATE...

  18. CDC STATE System Tobacco Legislation - Fire Safety

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Fire-Safety. The STATE...

  19. CDC STATE System Tobacco Legislation - Fire Safety

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Fire-Safety. The STATE...

  20. CDC WONDER: Mortality - Multiple Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Multiple Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2009. Data are based on...

  1. CDC WONDER: Mortality - Multiple Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Multiple Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2006. These data are...

  2. CDC Study Finds Fecal Contamination in Pools

    Science.gov (United States)

    ... Communication (404) 639-3286 CDC study finds fecal contamination in pools A study of public pools done ... The E. coli is a marker for fecal contamination. Finding a high percentage of E. coli-positive ...

  3. CDC STATE System Tobacco Legislation - Preemption Summary

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Preemption. The STATE...

  4. CDC STATE System Tobacco Legislation - Preemption

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Preemption. The STATE...

  5. CDC STATE System Tobacco Legislation - Smokefree Campus

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Campuses. The...

  6. CDC STATE System Tobacco Legislation - Tax

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation-Tax. The STATE System...

  7. CDC STATE System Tobacco Legislation - Tax

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation-Tax. The STATE System...

  8. CDC WONDER: Sexually Transmitted Disease (STD) morbidity

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sexually Transmitted Disease (STD) Morbidity online databases on CDC WONDER contain case reports reported from the 50 United States and D.C., Puerto Rico, Virgin...

  9. CDC WONDER: Sexually Transmitted Disease (STD) Morbidity

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sexually Transmitted Disease (STD) Morbidity online databases on CDC WONDER contain case reports reported from the 50 United States and D.C., Puerto Rico, Virgin...

  10. CDC WONDER: AIDS Public Use Data

    Data.gov (United States)

    U.S. Department of Health & Human Services — The AIDS Public Information Data Set (APIDS) for years 1981-2002 on CDC WONDER online database contains counts of AIDS (Acquired Immune Deficiency Syndrome) cases...

  11. CDC WONDER: Online Tuberculosis Information System (OTIS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Online Tuberculosis Information System (OTIS) on CDC WONDER contains information on verified tuberculosis (TB) cases reported to the Centers for Disease Control...

  12. CDC WONDER: Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CDC WONDER Mortality - Underlying Cause of Death online database is a county-level national mortality and population database spanning the years since 1979. Data...

  13. CDC WONDER: Daily Fine Particulate Matter

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Daily Fine Particulate Matter data available on CDC WONDER are geographically aggregated daily measures of fine particulate matter in the outdoor air, spanning...

  14. Internacionalização de PME no Continente Americano: Revisão da Literatura

    OpenAIRE

    Cláudia Pires Ribau; António Carrizo Moreira; Mário Raposo

    2018-01-01

    Dado el gran interés de la comunidad académica y el mundo empresarial sobre la internacionalización de las pequeñas y medianas empresas (pymes), esta investigación tiene como objetivo revisar la literatura empírica sobre la internacionalización de las pymes en el continente americano, a partir de las principales bases de datos académicas. Partiendo de un conjunto de 329 artículos, este estudio examina en detalle 39 artículos entre 1999 y 2014, lo que contribuye significativamente al desarroll...

  15. CDC Climat - 2011 Sustainable Development Report

    International Nuclear Information System (INIS)

    2012-08-01

    CDC Climat is the Caisse des Depots (CDC) subsidiary that is dedicated to combating climate change. Its activities aim to support the transition towards a low resource and low greenhouse gas emission (GHG) economy, through services that are cutting-edge, pro table, and in line with CDC's public policy goals. Through its corporate purpose, CDC Climat embodies the CDC's commitments in the sustainable development field. CDC Climat supports the implementation of public GHG emission reduction policies, primarily through emission trading schemes at the European and international level. Since it was founded in 2010, and throughout 2011, its strategic priorities have consisted in: - developing a long-term policy for investing in carbon credits generated by environmental initiatives, as part of the project mechanisms set up by the Kyoto Protocol, and used in the European Emission Trading Scheme; - supporting the development of its investments in carbon finance operators, like BlueNext, the European carbon exchange, for instance; - broadening the scope of its research into climate economics, which is supported by CDC and available to everyone, in order to serve the public and private players concerned. Its teams have supported French and European governments, international organisations and the United Nations, and various NGOs in their work and thinking on the future of tools for combating climate change. They have specifically contributed reports based on their research and operational feedback. When it was founded, CDC Climat was closely linked to public policies aimed at combating climate change via allowance and carbon trading mechanisms. The difficulties encountered by international negotiations, together with the effects of the economic and financial downturn in Europe, have resulted in a very pronounced fall in the price of carbon assets on these markets since the summer of 2011, with no prospect of recovery for several years. This environment is calling some of the

  16. Regulated degradation of the APC coactivator Cdc20

    Directory of Open Access Journals (Sweden)

    Robbins Jonathan A

    2010-09-01

    Full Text Available Abstract Background Cdc20 is a highly conserved activator of the anaphase-promoting complex (APC, promoting cell-cycle-regulated ubiquitination and proteolysis of a number of critical cell-cycle-regulatory targets including securin and mitotic cyclins. APC-Cdc20 activity is tightly regulated, and this regulation is likely important for accurate cell cycle control. One significant component of Cdc20 regulation is thought to be Cdc20 proteolysis. However, published literature suggests different mechanisms and requirements for Cdc20 proteolysis. The degree to which Cdc20 proteolysis is cell-cycle regulated, the dependence of Cdc20 proteolysis on Cdc20 destruction boxes (recognition sequences for APC-mediated ubiqutination, either by Cdc20 or by the related Cdh1 APC activator, and the need for APC itself for Cdc20 proteolysis all have been disputed to varying extents. In animals, Cdc20 proteolysis is thought to be mediated by Cdh1, contributing an intrinsic order of APC activation by Cdc20 and then by Cdh1. One report suggests a Cdh1 requirement for Cdc20 proteolysis in budding yeast; this idea has not been tested further. Results We characterized Cdc20 proteolysis using Cdc20 expressed from its endogenous locus; previous studies generally employed strongly overexpressed Cdc20, which can cause significant artifacts. We analyzed Cdc20 proteolysis with or without mutations in previously identified destruction box sequences, using varying methods of cell cycle synchronization, and in the presence or absence of Cdh1. Cdc20 instability is only partially dependent on destruction boxes. A much stronger dependence on Cdh1 for Cdc20 proteolysis was observed, but Cdh1-independent proteolysis was also clearly observed. Cdc20 proteolysis independent of both destruction boxes and Cdh1 was especially detectable around the G1/S transition; Cdh1-dependent proteolysis was most notable in late mitosis and G1. Conclusions Cdc20 proteolysis is under complex control

  17. Percepciones e interpretaciones sobre humanismo en el Museo del Hombre Americano

    Directory of Open Access Journals (Sweden)

    Isabela Soraia Backx Sanabria

    2015-01-01

    Full Text Available Este artículo presenta un análisis de la exposición permanente del Museo del Hombre Americano (Piauí - Brasil, institución dedicada a la comunicación de los resultados obtenidos en más de cuarenta años de investigaciones arqueológicas llevadas a cabo en el Parque Nacional de la Sierra de Capivara. El parque posee la mayor concentración de sitios arqueológicos de las Américas y fue inscrito por la UNESCO en 1991 en la lista del Patrimonio Cultural de la Humanidad. En su museo, así como en todos los otros, la articulación de la cultura material en las exposiciones construye varios discursos que son transmitidos al público por medio de recursos visuales, sonoros y táctiles. El presente trabajo se centra en el estudio de esas prácticas en el Museo del Hombre Americano, analizando los discursos sobre el Hombre y el humanismo producidos en esa institución.

  18. Retornados e inadaptados: el "Americano" Gallego, entre mito y realidad (1880-1930

    Directory of Open Access Journals (Sweden)

    Núñez Seixas, Xosé M.

    1998-12-01

    Full Text Available During the period of mass migration to America, return migrants, also labelled as "americans" or "indianos", played an important role in Galicia's rural regions as technical innovators and catalysers of the articulation of civil society. This article attemps to have a first look on the topic, by researching into the internal diversity of the whole collectivity of return migrants. It also aims at offering a typology of the social and political agency of return migrants in Galicia in comparative perspective.

    En el período de emigración masiva hacia América, los retornados, también denominados "americanos" o "indianos", jugaron un papel importante como introductores de innovaciones técnicas y como catalizadores de la articulación de la sociedad civil en las zonas rurales de Galicia. Este artículo intenta una aproximación al tema, preguntándose por la diversidad interna del colectivo de retornados y ensayando, en virtud de la comparación con otros ejemplos históricos, una tipología de la agencia social y política de los "americanos" gallegos.

  19. Pruebas de comportamiento caótico en índices bursátiles americanos

    Directory of Open Access Journals (Sweden)

    Franco Parisi

    2007-01-01

    Full Text Available Este artículo valida el comportamiento caótico en las Bolsas de Valores de Argentina, Brasil, Canadá, Chile, Estados Unidos, Perú y México utilizando los índices accionarios Merval, Bovespa, S&P TSX Composite, IPSA, IGPA, S&P 500, Dow Jones Industrials, Nasdaq, IGBVL e IPC, respectivamente. Los resultados de distintas técnicas y métodos como análisis gráfico, análisis de recurrencia, entropía de espacio temporal, coeficiente de Hurst, exponente de Lyapunov y dimensión de correlación, apoyan la hipótesis de que los mercados bursátiles americanos se comportan de forma caótica, en contra de la hipótesis de mercados eficientes y la hipótesis de aleatoriedad. Esta conclusión valida el uso de instrumentos predictivos de rendimientos accionarios en los mercados de renta variable americanos. Destacable es el resultado de la técnica coeficiente de Hurst, que en promedio fue de 0.75 para los índices en estudio, lo que estaría justificando la utilización de modelos tipo Arfima, entre otros, para la predicción de dichas series.

  20. El AFRICOM: Qué lugar ocupa África en el dispositivo militar americano

    Directory of Open Access Journals (Sweden)

    María Ángeles Alaminos

    2008-10-01

    Full Text Available El nuevo interés americano por África y la reciente importancia geoestratégica del continente plantean cuestiones fundamentales desde el punto de vista militar y estratégico para los Estados Unidos. Tras años de largo debate y procesos de discusión en el seno del Departamento de Defensa y en los círculos académicos y militares, se decidió la creación de un nuevo mando, el AFRICOM, cuya área de responsabilidad la constituiría todo el continente africano y aguas circundantes, con la excepción de Egipto, que queda bajo el CENTCOM. El establecimiento del AFRICOM es más que la reorganización administrativa del Unified Command Plan; es la muestra de la creciente importancia geopolítica de África para los intereses americanos. Sin embargo, la resistencia africana al AFRICOM y al establecimiento del Cuartel General del mando en África ha sido grande, mostrando los países su rechazo a la presencia de cientos de militares en su territorio. La práctica totalidad de los Estados africanos considera el establecimiento del AFRICOM como una estrategia encubierta para proseguir con la agenda americana de la Guerra contra el Terror, así como una forma de asegurarse el acceso a los recursos naturales del continente.

  1. Cdc42-mediated tubulogenesis controls cell specification

    DEFF Research Database (Denmark)

    Kesavan, Gokul; Sand, Fredrik Wolfhagen; Greiner, Thomas Uwe

    2009-01-01

    Understanding how cells polarize and coordinate tubulogenesis during organ formation is a central question in biology. Tubulogenesis often coincides with cell-lineage specification during organ development. Hence, an elementary question is whether these two processes are independently controlled......, or whether proper cell specification depends on formation of tubes. To address these fundamental questions, we have studied the functional role of Cdc42 in pancreatic tubulogenesis. We present evidence that Cdc42 is essential for tube formation, specifically for initiating microlumen formation and later...... for maintaining apical cell polarity. Finally, we show that Cdc42 controls cell specification non-cell-autonomously by providing the correct microenvironment for proper control of cell-fate choices of multipotent progenitors. For a video summary of this article, see the PaperFlick file with the Supplemental Data...

  2. CDC Vital Signs: Teen Drinking and Driving

    Science.gov (United States)

    ... every trip, no matter how short. Obey speed limits. Never use a cell phone or text while driving. Parents can Understand that most teens ... teen passengers Never use a cell phone or text while driving Obey speed limits Get your copy of CDC's parent-teen driving ...

  3. CDC Vital Signs: Preventing Teen Pregnancy

    Science.gov (United States)

    ... 0:60 seconds] On Other Web Sites MedlinePlus – Teenage Pregnancy MedlinePlus – Birth Control The Doctor’s Channel: CDC Vital Signs Long Acting Reversible Contraception [VIDEO – 3:30 minutes] Top of Page Get Email Updates To receive ... USA.gov TOP

  4. CDC Vital Signs: Where's the Sodium?

    Science.gov (United States)

    ... Other Web Sites MedlinePlus – Heart Diseases MedlinePlus – Coronary Artery Disease MedlinePlus – High Blood Pressure MedlinePlus – Dietary Sodium ... Privacy FOIA No Fear Act OIG 1600 Clifton Road Atlanta , GA 30329-4027 USA 800-CDC-INFO ( ...

  5. CDC Best Practices for Comprehensive Tobacco Control Programs - 2007

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). Best Practices for Comprehensive Tobacco Control Programs. Funding. CDC's Best Practices for Comprehensive Tobacco...

  6. CDC Best Practices for Comprehensive Tobacco Control Programs - 2014

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). Best Practices for Comprehensive Tobacco Control Programs. Funding. CDC's Best Practices for Comprehensive Tobacco...

  7. cdc-25.4, a Caenorhabditis elegans Ortholog of cdc25, Is Required for Male Mating Behavior

    Directory of Open Access Journals (Sweden)

    Sangmi Oh

    2016-12-01

    Full Text Available Cell division cycle 25 (cdc25 is an evolutionarily conserved phosphatase that promotes cell cycle progression. Among the four cdc25 orthologs in Caenorhabditis elegans, we found that cdc-25.4 mutant males failed to produce outcrossed progeny. This was not caused by defects in sperm development, but by defects in male mating behavior. The cdc-25.4 mutant males showed various defects during male mating, including contact response, backing, turning, and vulva location. Aberrant turning behavior was the most prominent defect in the cdc-25.4 mutant males. We also found that cdc-25.4 is expressed in many neuronal cells throughout development. The turning defect in cdc-25.4 mutant males was recovered by cdc-25.4 transgenic expression in neuronal cells, suggesting that cdc-25.4 functions in neurons for male mating. However, the neuronal morphology of cdc-25.4 mutant males appeared to be normal, as examined with several neuronal markers. Also, RNAi depletion of wee-1.3, a C. elegans ortholog of Wee1/Myt1 kinase, failed to suppress the mating defects of cdc-25.4 mutant males. These findings suggest that, for successful male mating, cdc-25.4 does not target cell cycles that are required for neuronal differentiation and development. Rather, cdc-25.4 likely regulates noncanonical substrates in neuronal cells.

  8. Estado actual de la bartonellosis (fiebre verrucosa. verruga en el continente americano

    Directory of Open Access Journals (Sweden)

    Luis Patiño Camargo

    1940-09-01

    Full Text Available Trabajo Presentado al VIII Congreso Científico Panamericano reunido en Washington D.C. en mayo de 1940 por La grave calamidad que ha constituido para Colombia la epidemia de Bartonellosis o Fiebre Verrucosa en una región septentrional de su territorio; la endemia legendaria de el Perú; el hecho de haberse señalado en los últimos días focos activos en el Ecuador; y la circunstancia verosímil de que las remotas epidemias llamadas "Bubas" en Guatemala hayan sido de Verruga, demuestran que sobre un dilatado territorio, en varias naciones tributarias del Pacífico existen focos de Bartonellosis en el Continente Americano.

  9. Matizes do pensamento político norte-americano na virada conservadora de 1970

    Directory of Open Access Journals (Sweden)

    Maria Ribeiro do Valle

    Full Text Available Este artigo examina de que forma o revigoramento da excepcional experiência histórica americana aparece como solução para alguns dos principais neoconservadores, sendo emblemáticos Nathan Glazer e Irving Kristol, frente à "queda" do império americano, que tem início nos anos 1960 e 1970, depois de sua ascensão no período da Guerra Fria, os chamados 25 anos gloriosos. Constatamos que a argumentação desses autores está enraizada na retomada da retórica reacionária como antídoto à decadência da virtude americana supostamente provocada pelo comunismo, pelo movimento da contracultura e pelo alegado efeito perverso do welfare state.

  10. La CIDIP-VII y la defensa del consumidor americano. Aportes desde la experiencia del MERCOSUR

    Directory of Open Access Journals (Sweden)

    Cristian Ricardo A. Piris

    2014-03-01

    Full Text Available En el presente artículo se aborda la situación del consumidor ante la expansión de los mercados y por ende del consumo, junto con las consecuencias que trae aparejadas esta situación. En ese contexto, se lleva a cabo un análisis de la normativa que podría llegar a ser aplicable en cada ocasión en la que haga falta regular alguna conducta o situación determinada en el continente Americano. Luego, se hace hincapié en la CIDIP VII, siendo que constituye un componente principal de la OEA en el desarrollo del Derecho Internacional Privado en América y cumple un papel muy importante en la codificación y armonización de este derecho en la región.

  11. Repertorio Americano 1974-1982. Edición de OCTUBRE-NOVIEMBRE-DICIEMBRE-1981

    OpenAIRE

    Instituto de Estudios Latinoamericanos, UNA

    1981-01-01

    Manuel Arce, El libro de la patria. Un hito en la evolución poética de Alfonso Chase, 1* Sin autor, Fotografía, 1* Sin autor, La Habana, Cuba, con los poetas Adolfo Martí Fuentes, Luis Suardíaz, Angel Augier y Nicolás Guillén, 3* Sin autor, Repertorio Americano, 4* J. G., Semblanza del maestro Joaquín García Monge, 4* Carlos Enrique Aguirre Gómez, Ars poética y poesía en “la piel de los signos” de Mario Picado, 5* Sin autor, El poeta Mario Picado con el también poeta para Julián Marchena, 5* ...

  12. Las influencias europeas en el movimiento plástico del expresionismo-abstracto americano

    Directory of Open Access Journals (Sweden)

    Amparo Serrano de Haro Soriano

    1991-01-01

    Full Text Available La literatura crítica que gira en torno al Expresionismo-Abstracto norteamericano (que es la tendencia artística predominante en EEUU en los años 40 y 50 de nuestro siglo tiene dos fases principales. En primer lugar una serie de libros que aparecen a partir de los años 50 y 60 de contenido laudatorio, exaltante y reafirmando lo «americano» de este arte, los títulos son por sí solos extremadamente ilustrativos: Rudy Blesh Modem Art USA: Men, Rebellion, Conquest de 1956; Thomas B. Hess Abstract Painting: Background and American Phase de 1951; Irving Sandler The Triumph of American Painting: A History of Abstract Expressionism de 1970; William Seitz Abstract Expressionism Painting in America de 1955.

  13. 13 CFR 120.851 - CDC ethical requirements.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false CDC ethical requirements. 120.851... Company Loan Program (504) Other Cdc Requirements § 120.851 CDC ethical requirements. CDCs and their Associates must act ethically and exhibit good character. They must meet all of the ethical requirements of...

  14. Cdc48: A Swiss Army Knife of Cell Biology

    Directory of Open Access Journals (Sweden)

    Guem Hee Baek

    2013-01-01

    Full Text Available Cdc48 (also called VCP and p97 is an abundant protein that plays essential regulatory functions in a broad array of cellular processes. Working with various cofactors, Cdc48 utilizes its ATPase activity to promote the assembly and disassembly of protein complexes. Here, we review key biological functions and regulation of Cdc48 in ubiquitin-related events. Given the broad employment of Cdc48 in cell biology and its intimate ties to human diseases (e.g., amyotrophic lateral sclerosis, studies of Cdc48 will bring significant insights into the mechanism and function of ubiquitin in health and diseases.

  15. Cdc42 GTPase dynamics control directional growth responses

    Science.gov (United States)

    Brand, Alexandra C.; Morrison, Emma; Milne, Stephen; Gonia, Sara; Gale, Cheryl A.; Gow, Neil A. R.

    2014-01-01

    Polarized cells reorient their direction of growth in response to environmental cues. In the fungus Candida albicans, the Rho-family small GTPase, Cdc42, is essential for polarized hyphal growth and Ca2+ influx is required for the tropic responses of hyphae to environmental cues, but the regulatory link between these systems is unclear. In this study, the interaction between Ca2+ influx and Cdc42 polarity-complex dynamics was investigated using hyphal galvanotropic and thigmotropic responses as reporter systems. During polarity establishment in an applied electric field, cathodal emergence of hyphae was lost when either of the two Cdc42 apical recycling pathways was disrupted by deletion of Rdi1, a guanine nucleotide dissociation inhibitor, or Bnr1, a formin, but was completely restored by extracellular Ca2+. Loss of the Cdc42 GTPase activating proteins, Rga2 and Bem3, also abolished cathodal polarization, but this was not rescued by Ca2+. Expression of GTP-locked Cdc42 reversed the polarity of hypha emergence from cathodal to anodal, an effect augmented by Ca2+. The cathodal directional cue therefore requires Cdc42 GTP hydrolysis. Ca2+ influx amplifies Cdc42-mediated directional growth signals, in part by augmenting Cdc42 apical trafficking. The Ca2+-binding EF-hand motif in Cdc24, the Cdc42 activator, was essential for growth in yeast cells but not in established hyphae. The Cdc24 EF-hand motif is therefore essential for polarity establishment but not for polarity maintenance. PMID:24385582

  16. THE CDC-CDCR DOCUMENTATION PROJECT.

    Science.gov (United States)

    WADDELL, M S

    1964-01-01

    A progress report on an experiment in mechanized methods for the storage and retrieval of communicable diseases information. The project, supported by National Institutes of Health grants and begun in 1958, is being conducted by the Center for Documentation and Communication Research (CDCR), School of Library Science, Western Reserve University, Cleveland, Ohio, in cooperation with the Communicable Disease Center (CDC), United States Public Health Service, Atlanta, Georgia. Initial phases have been completed. Seventy-seven questions submitted by the CDC Documentation Committee have been searched by CDCR on their GE-225 computer. For seventy-three of the seventy-seven questions, the system responded with at least one appropriate answer. The combined pertinent and peripheral responses totaled 94.7 percent. The file of abstracted literature is being increased preparatory to large-scale testing. Further investigation and evaluation are necessary before the project can be fairly appraised.

  17. CDC Vital Signs-Heroin Epidemic

    Centers for Disease Control (CDC) Podcasts

    2015-07-07

    This podcast is based on the July 2015 CDC Vital Signs report. Heroin use and heroin-related overdose deaths are increasing. Most people are using it with other drugs, especially prescription opioid painkillers. Learn what can be done to prevent and treat the problem.  Created: 7/7/2015 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 7/7/2015.

  18. CDC Vital Signs-Preventing Melanoma

    Centers for Disease Control (CDC) Podcasts

    2015-06-02

    This podcast is based on the June 2015 CDC Vital Signs report. Skin cancer is the most common form of cancer in the U.S. In 2011, there were more than 65,000 cases of melanoma, the most deadly form of skin cancer. Learn how everyone can help prevent skin cancer.  Created: 6/2/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 6/2/2015.

  19. O uso correto de nomes vernaculares dos botos sul-americanos do gênero Sotalia Gray (Mammalia, Cetacea, Delphinidae)

    OpenAIRE

    Geise, Lena

    2006-01-01

    The usage of the vernacular names for South American dolphins genus Sotalia Gray, 1866 is discussed in accordance to geographic characteristics of local people identification.O uso de nomes vernaculares para os botos sul-americanos do gênero Sotalia Gray, 1866 são discutidos de acordo com as características geográficas das identificações feitas pelas populações litorâneas.

  20. Políticas de amizade: Portinari e o mundo cultural ibero-americano

    Directory of Open Access Journals (Sweden)

    Maria de Fátima Fontes Piazza

    Full Text Available O presente artigo visa mostrar uma rede de sociabilidade intelectual formada a partir das relações entre o pintor Cândido Portinari (1903-1962 com o mundo cultural ibero-americano, especialmente com o poeta espanhol Rafael Alberti, com o crítico de arte argentino Jorge Romero Brest e com os uruguaios, Carlos Washington Aliseris, pintor, Cipriano Santiago Vitureira, escritor e crítico de arte, e Enrique Amorim, poeta e escritor, entre outros. Aqui, as correspondências foram analisadas como "objeto cultural", seguindo a trilha dos estudos desenvolvidos sob a perspectiva da história dos intelectuais segundo a tradição francesa. Tais missivas revelaram que para a cidade de Buenos Aires acorreu um significativo número de exilados de várias partes da Ibero-América e da Europa (com ênfase nos espanhóis, o que levou a cosmopolita capital portenha a figurar como um promissor mercado de bens culturais.

  1. Estabilidade monetária e CEPAL: A heterogeneidade do pensamento estruturalista latino-americano

    Directory of Open Access Journals (Sweden)

    PEDRO LUIZ APRIGIO

    2018-03-01

    Full Text Available RESUMO O artigo verifica a hipótese de homogeneidade estrita do pensamento estruturalista latino-americano em matéria de estabilidade monetária em seus anos iniciais. Em meados da década de 1950, o crescente e contínuo fenômeno inflacionário na região era explicado pela CEPAL como resultante da presença de estrangulamentos nas estruturas reais da economia. Entretanto, o canal de transmissão ao nível de preços é explicado pelos autores de maneiras diversas, gerando-se, por conseguinte, diferentes propostas de estabilização. O artigo sublinha a flexibilidade metodológica e o grau de fragmentação teórica da abordagem cepalina, bem como a controvérsia sobre a inflação constituir parte do processo de desenvolvimento. A análise destes elementos revela uma tensão entre os aspectos keynesianos anglo-saxônicos na abordagem de Prebisch e a original teoria latino-americana proposta por Noyola e Furtado. Avalia-se em que medida este descompasso impediu a constituição de um corpo teórico diferenciado em matéria de estabilização monetária.

  2. Internacionalização de PME no Continente Americano: Revisão da Literatura

    Directory of Open Access Journals (Sweden)

    Cláudia Pires Ribau

    2018-01-01

    Full Text Available Dado el gran interés de la comunidad académica y el mundo empresarial sobre la internacionalización de las pequeñas y medianas empresas (pymes, esta investigación tiene como objetivo revisar la literatura empírica sobre la internacionalización de las pymes en el continente americano, a partir de las principales bases de datos académicas. Partiendo de un conjunto de 329 artículos, este estudio examina en detalle 39 artículos entre 1999 y 2014, lo que contribuye significativamente al desarrollo del conocimiento y la comprensión de la investigación realizada hasta la fecha en este ámbito. Con este análisis se han identificado tendencias y patrones, y se concluye que la investigación realizada sobre la internacionalización de pymes se centra en los EE. UU. y Canadá, influenciada principalmente en cuatro temas principales: las teorías de la internacionalización, estrategia, desempeño y perspectiva internacional. Igualmente, se concluye que las investigaciones han seguido un predominio cuantitativo. Las conclusiones e interpretaciones de oportunidades para futuras investigaciones son relevantes tanto para la comunidad académica como para el mundo empresarial.

  3. Compositores Latino-Americanos and New Paradigms of the 20th Century Classical Music

    Directory of Open Access Journals (Sweden)

    Eliana Monteiro da Silva

    2013-12-01

    Full Text Available This article discusses the direction taken by some Latin American composers in light of the new tendencies aimed to challenge paradigms of traditional Western classical music during the 20th century. Latin American composers were influenced by these tendencies to a greater or lesser degree depending on the place and time they lived. They worked with different styles and techniques while attempting to maintain a dialogue with the production of other contemporary artists without losing their identity. Some of these practices are shown in this paper. The examples were taken from a set of 54 pieces for piano recorded by Beatriz Balzi, an Argentinian-Brazilian pianist, contained in her album entitled Compositores Latino-americanos [Latin American Composers]. Exemplifying 13 Latin American countries, this repertoire was analyzed by the authors in order to define the most common practices adopted and/or created by the composers chosen by Balzi. These practices were then catalogued into six topics and organized in chronological order of events and styles to which they relate. To describe the main tendencies mentioned in this paper the authors adopted Stefan Kostka's Materials and Techniques of Twentieth Century Music for his clear, objective writing. This work helped to contextualize Balzi's repertoire, comparing the Latin American compositional process to some of the most respected musicians of the Western world.

  4. Dinâmica, determinações e sistema mundial no desenvolvimento do capitalismo nos termos de Theotonio dos Santos: da teoria da dependência à teoria dos sistemas-mundo

    OpenAIRE

    Baptista Filho, Almir Cézar de Carvalho

    2009-01-01

    A dissertação discute a dinâmica de longo prazo, as determinações e a hierarquização do capitalismo mundial na obra de Theotonio dos Santos. O interesse por esta obra justifica-se duplamente pela importância da mesma no contexto do pensamento social latino-americano, e por seu percurso teórico, transitando entre duas das vertentes da teoria do desenvolvimento: a Teoria Marxista da Dependência ( da qual foi co-fundador; e a Teoria dos Sistemas-mundo (TSM), que se tornou um dos m...

  5. CDC Vital Signs-Hispanic Health

    Centers for Disease Control (CDC) Podcasts

    2015-05-05

    This podcast is based on the May 2015 CDC Vital Signs report. About one in six people living in the U.S. are Hispanic. The two leading causes of death in this group are heart disease and cancer, accounting for two out of five deaths. Unfortunately, many Hispanics face considerable barriers to getting high quality health care, including language and low income. Learn what can be done to reduce the barriers.  Created: 5/5/2015 by Office of Minority Health & Health Equity (OMHHE).   Date Released: 5/5/2015.

  6. CDC Vital Signs-Heart Age

    Centers for Disease Control (CDC) Podcasts

    2015-09-01

    This podcast is based on the September 2015 CDC Vital Signs report. Your heart age is the age of your heart and blood vessels as a result of your risk factors for heart attack and stroke. If you smoke or have high blood pressure, your heart age will be much higher than your actual age. Learn what you can do to lower your heart age and keep it low.  Created: 9/1/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/1/2015.

  7. Perseguir al SRAG: CDC en acción (Stalking SARS: CDC at Work)

    Centers for Disease Control (CDC) Podcasts

    2013-04-29

    En este podcast los niños de Kidtastics hablan sobre el brote del SRAS y cómo trabajaron los CDC para resolver el misterio.  Created: 4/29/2013 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 8/10/2016.

  8. Human CDT1 associates with CDC7 and recruits CDC45 to chromatin during S phase

    DEFF Research Database (Denmark)

    Ballabeni, Andrea; Zamponi, Raffaela; Caprara, Greta

    2009-01-01

    The initiation of DNA replication is a tightly controlled process that involves the formation of distinct complexes at origins of DNA replication at specific periods of the cell cycle. Pre-Replicative Complexes are formed during telophase and early G1. They rearrange at the start of S phase to fo...... to chromatin is regulated by CDC7. We propose a model in which chromatin bound CDT1 is first stabilized and subsequently displaced by CDC7 activity, thereby ensuring the timely execution of DNA replication.......The initiation of DNA replication is a tightly controlled process that involves the formation of distinct complexes at origins of DNA replication at specific periods of the cell cycle. Pre-Replicative Complexes are formed during telophase and early G1. They rearrange at the start of S phase to form...... pre-Initiation Complexes, which are a prerequisite for DNA replication. The CDT1 protein is required for the formation of the pre-Replicative Complexes. Here we show that human CDT1 associates with the CDC7 kinase and recruits CDC45 to chromatin. Moreover, we show that the amount of CDT1 bound...

  9. CDC Health Disparities and Inequalities Report--U.S. 2013

    Science.gov (United States)

    ... of Epidemiology, Analysis, and Library Services (DEALS) Disparities Analytics CDC Disability and Health, Health Care Data & Statistics Healthy People 2020 HHS National Partnership for Action (NPA) AHRQ ...

  10. Cdc42 promotes host defenses against fatal infection

    DEFF Research Database (Denmark)

    Lee, Keunwook; Boyd, Kelli L; Parekh, Diptiben V

    2013-01-01

    attempted to specifically delete it in these cells by crossing the Cdc42(fl/fl) mouse with a FSP-1 cre mouse, which is thought to mediate recombination exclusively in fibroblasts. Surprisingly, the FSP-1cre;Cdc42(fl/fl) mice died at 3 weeks of age due to overwhelming suppurative upper airway infections...... showed that in addition to fibroblasts, the FSP-1 cre deleted Cdc42 very efficiently in all leukocytes. Thus, by using this non-specific cre mouse we inadvertently demonstrated the importance of Cdc42 in host protection from lethal infections and suggest a critical role for this small GTPase in innate...

  11. Isolation of a cdc28 mutation that abrogates the dependence of S ...

    Indian Academy of Sciences (India)

    We have isolated a mutation in the budding yeast Saccharomyces cerevisisae CDC28 gene that allows cdc13 cells, carrying damaged DNA, to continue with the cell division cycle. While cdc13 mutant cells are arrested as large-budded cells at the nonpermissive temperature 37°C, the cdc13 cdc28 double mutant culture ...

  12. Dialogismo: emergência do pensamento latino-americano em comunicação

    Directory of Open Access Journals (Sweden)

    Yuji Gushiken

    2008-09-01

    Full Text Available Neste artigo estuda-se a emergência do pensamento latino-americano em comunicação, tendo como foco a proposta dialógica que, na história das teorias da comunicação, se caracteriza como crítica à mass communication research da sociologia americana. Situa a emergência desse pensamento comunicacional na América Latina em meio a condições de subdesenvolvimento econômico e social, analisando-o também como crítica aos difusionismos culturais e comunicacionais do processo de globalização. Historicamente, o dialogismo atualiza os processos de inovação teórica que reavaliaram a recepção como mediadora e produtora de sentidos nos estudos em comunicação. Palavras-chave: Comunicação; dialogismo; América Latina. ABSTRACT In this article, latin-american thought emergence in communication is studied focusing on the dialogue proposal which, in the history of communication theories, is characterised as a critique of mass communication research in American sociology. Latin-american thought emergence in communication is situated within conditions of economic and socialunderdevelopment being analysed also as a critique of the idea of cultural and communication dispersion of the globalisation process. Historically, dialogism updates the processes of theory innovation which reassesses reception as a mediator and producer of meaning in the studies of communication. Keywords: Communication; dialogism; Latin America.

  13. El imaginario flamenco americano: Aura y Kitsch en la escena transnacional

    Directory of Open Access Journals (Sweden)

    Asensio, Susana

    2004-12-01

    Full Text Available The continuous transformations suffered by flamenco have always been related to its interactions with many other traditions. What once was a local experience, although hybrid from its origins, was transformed into a chameleonic musical phenomenon able to produce new development while conserving a characteristic personality. The transnational flamenco progress started around the end of 19th century. It is considered that its trips to America, especially those to the United States, were the most relevant to the phenomenon. However, flamenco artists developed their careers detached from its socio-cultural environment —and even from American cultures— in the new settings. Their isolation was twofold and favored specific ways to perform and experience flamenco. This new expansion gave transnational flamenco a distinctive and contemporary Kitsch touch.

    Las continuas transformaciones sufridas por el flamenco han estado siempre relacionadas con su interacción con innumerables tradiciones. Lo que una vez fue un fenómeno local, aunque híbrido desde su origen, se convierte en un fenómeno musical camaleónico, capaz de producir fenómenos nuevos al tiempo que sigue manteniendo su personalidad distintiva. El movimiento transnacional del flamenco comenzó ya en el siglo XIX, aunque podemos considerar que su viaje a los Estados Unidos es el que más relevancia ha tenido. Allí, sin embargo, los artistas desarrollaron sus carreras no sólo aislados del entorno sociocultural flamenco, sino también de la cultura popular americana. Su aislamiento fue doble y así los artistas americanos desarrollaron maneras específicas de interpretar y experienciar el flamenco representativas del Kitsch más contemporáneo.

  14. HEDGE FINANCEIRO PARA OPERAÇÕES EM DÓLAR AMERICANO

    Directory of Open Access Journals (Sweden)

    Ângela Maria Carneiro Silva

    2014-01-01

    Full Text Available O processo de globalização dos mercados tem influenciado de maneira bastante ampla o modelo de operação das empresas, tanto por meio da ampliação da oferta de fornecedores de insumos quanto pelo aumento de clientes no exterior. Este novo contexto tem propiciado vários benefícios, dentre eles o aumento de vendas e a redução dos custos dos insumos, como também tem exposto estas empresas a riscos adicionais importantes, como o risco cambial. Ao longo do tempo e por influência do desenvolvimento dos mercados de valores, começaram a ser desenvolvidas ferramentas destinadas à proteção das operações de compra e venda de ativos, como contratos a termo, contratos futuros e opções, o que permitiu o desenvolvimento de várias estratégias de proteção, ou hedging. Com o objetivo de apresentar uma estratégia de hedge por meio de contratos futuros para operações de vendas em dólares americanos, estaremos utilizando a técnica de hedge de variância mínima aplicada sobre um fluxo de recebimento de contratos de uma empresa exportadora. Para esta aplicação foram utilizadas as cotações no mercado à vista e futuros negociados na Bolsa de Mercadoria e Futuros (BM&F.

  15. CDC Vital Signs–Opioid Prescribing

    Centers for Disease Control (CDC) Podcasts

    2017-07-06

    This podcast is based on the July 2017 CDC Vital Signs report. Higher opioid prescribing puts patients at risk for addiction and overdose. Learn what can be done about this serious problem.  Created: 7/6/2017 by Centers for Disease Control and Prevention (CDC).   Date Released: 7/6/2017.

  16. Top syndicated pages from CDC.gov by weekly page views

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CDC Content Syndication site at https://tools.cdc.gov/syndication/ allows you to import content from CDC websites directly into your own website or application....

  17. Prácticas escolares en el Colégio Americano: un enfoque en la cultura material

    Directory of Open Access Journals (Sweden)

    Marlucy do Socorro Aragão de Sousa

    2017-05-01

    Full Text Available Este artículo aborda las prácticas escolares y la cultura material de la escuela en Belém do Pará, a finales del siglo XIX. El objetivo es analizar cómo se configuraron la cultura material y las prácticas escolares en el Colégio Americano, ideado por José Veríssimo entre 1884 y 1890. Se usa como fuente la obra Noticia Geral sobre o Collegio Americano. Se utilizó la metodología histórico-hermenéutica. Al analizar la cultura material de la escuela, se tiene como horizonte la percepción de sus vínculos con el contexto histórico, cultural y social en el que se generó un nuevo modelo de educación que destacaba la civilidad, la nación y la moral. Por consiguiente, dichos artefactos no eran simples objetos, sino que revelaron las relaciones conflictivas o pacíficas de aquel contexto, e incluso las prácticas escolares de la institución. Se observa que el uso y la descripción de los objetos dispuestos en la estructura del Colégio Americano, para su creador, tenían el objetivo de establecer y mantener normas que debían formar hombres disciplinados, que se vincularan directamente con el propósito de la educación física, moral y cívica de los estudiantes.

  18. O DIREITO FUNDAMENTAL AO MEIO AMBIENTE NO NOVO CONSTITUCIONALISMO LATINO-AMERICANO: BOLÍVIA E EQUADOR

    Directory of Open Access Journals (Sweden)

    Ana Cristina Willemann

    2013-11-01

    constitucional que vem se desenvolvendo nos países latinos; e (ii abordar o direito ao meio ambiente na nova visão do constitucionalismo latino-americano; e analisar como o respeito a Pacha Mama, Madre Tierra, impõe o direito fundamental à água e aos outros seres vivos. A metodologia utilizada é a revisão bibliográfica por meio de leitura e análise comparada dos textos dos autores mencionados na bibliografia deste artigo.

  19. El laberinto teatral de espejos digitales : la presentación de mexicano-americanos en Facebook

    OpenAIRE

    Arao Galhardi, Renato de Almeida

    2010-01-01

    Este trabajo explora las formas en las cuales mexicano-americanos universitarios actualmente viviendo en Estados Unidos, se presentan en un sitio de redes sociales, Facebook. Partiendo de los enfoques del interaccionismo simbólico, la fenomenología y la psicología social, investigando cómo éstos incorporan la mexicanidad dentro de sus presentaciones digitales. 1. Definición del marco analítico. -- 2. Internet: la red de redes. -- 3. Una introducción a Facebook. -- 4. Analogías digitales: s...

  20. SISTEMA INTERAMERICANO, COOPERAÇÃO E A COMPETIÇÃO INTRAREGIONAL

    Directory of Open Access Journals (Sweden)

    Sabrina Evangelista Medeiros

    2014-12-01

    Full Text Available Este texto tem como propósito a análise do sistema inter-americano e da Organização dos Estados Americanos por meio de sua história e componentes relacionadas às tratativas e organicidade do sistema. Para isso, esta abordagem tem como base a sua evolução institucional, os diferentes usos conceituais e de propósito, as principais fases deste sistema e, em consequência, a reputação associada a ele em que pese os marcos regulatórios e compromissos assumidos no âmbito da OEA e fora dele. A hipótese é de que, a OEA teve constante evolução institucional por conta da transição de objetivos que sofreu entre o fim da Guerra Fria e o século XXI e que, nesta conjuntura e como forma de adaptar-se às outras frentes regionais potencialmente competitivas, volta-se para a questão da segurança individual e direitos humanos criando um guarda-chuva em que cabem, de maneira complementar, as demais iniciativas setorialmente organizadas, como as de finalidade energética, comercial ou de defesa. Por último, que a configuração do setor de defesa nestes mesmos marcos regulatórios criados ao longo destes anos apresenta-se pela fusão de fatores multidimensionais, que vão desde a segurança civil às fronteiras, com poucos aspectos tradicionais de defesa, realocando o seu papel na região

  1. Cdc7 kinase - a new target for drug development.

    Science.gov (United States)

    Swords, Ronan; Mahalingam, Devalingam; O'Dwyer, Michael; Santocanale, Corrado; Kelly, Kevin; Carew, Jennifer; Giles, Francis

    2010-01-01

    The cell division cycle 7 (Cdc7) is a serine threonine kinase that is of critical importance in the regulation of normal cell cycle progression. Cdc7 kinase is highly conserved during evolution and much has been learned about its biological roles in humans through the study of lower eukaryotes, particularly yeasts. Two important regulator proteins, Dbf4 and Drf1, bind to and modulate the kinase activity of human Cdc7 which phosphorylates several sites on Mcm2 (minichromosome maintenance protein 2), one of the six subunits of the replicative DNA helicase needed for duplication of the genome. Through regulation of both DNA synthesis and DNA damage response, both key functions in the survival of tumour cells, Cdc7 becomes an attractive target for pharmacological inhibition. There are much data available on the pre-clinical anti-cancer effects of Cdc7 depletion and although there are no available Cdc7 inhibitors in clinical trials as yet, several lead compounds are being optimised for this purpose. In this review, we will address the current status of Cdc7 as an important target for new drug development.

  2. en tres sistemas agroforestales

    Directory of Open Access Journals (Sweden)

    Javier Monge Meza

    2006-01-01

    Full Text Available Se estudió el desarrollo inicial (30 meses del duraznillo (Prunus annularis en tres sistemas agroforestales: duraznillo-maíz (Zea mays, duraznillomenta (Satureja viminea y duraznillo-naranjilla (Solanum quitoense, en un sitio en la zona de vida Bosque muy húmedo Montano Bajo, en Costa Rica. Se utilizaron cuatro parcelas de cada sistema agroforestal, con una densidad de 722 árboles/ ha, y un sistema de siembra de árboles en forma de triángulo equilátero, entre los que se intercaló el componente agrícola respectivo de cada sistema agroforestal. La altura media alcanzada por los árboles de duraznillo fue de 226,2, 221,4 y 164,3 cm, para el sistema agroforestal duraznillo-maíz, duraznillo- menta y duraznillo-naranjilla, respectivamente. En cuanto a los volúmenes, éstos variaron entre 0,58 y 1,30 m3/ha. Hubo una diferencia significativa en altura media entre el sistema agroforestal duraznillo-maíz y duraznillo-menta con respecto al de duraznillo-naranjilla. No hubo diferencias con respecto al volumen entre los sistemas de producción

  3. Um exemplo de inversão do domínio ideológico norte-americano no Brasil

    OpenAIRE

    Carla Damião

    2014-01-01

    Propomos discutir a relação de domínio ideológico-cultural norte-americano sobre o Brasil, tendo em vista exemplos documentados. Trata-se da chamada “política de boa vizinhança” [Good Neighbor Policy], com início em 1933 até o final da Segunda Guerra, notadamente durante o governo Getúlio Vargas no Brasil e o governo Franklin Roosevelt nos Estados Unidos. A necessidade de propaganda política passava pela “sedução” cultural da potência estadunidense sobre seus vizinhos latino-americanos. Neste...

  4. Sistema Socioeducativo Potiguar: um debate sobre violações de direitos de crianças e adolescentes em âmbito internacional

    Directory of Open Access Journals (Sweden)

    Ilana Lemos Paiva

    2016-12-01

    Full Text Available O Estatuto da Criança e do Adolescente (ECA, embasado especialmente no processo da Convenção Internacional das Nações Unidas sobre os Direitos da Criança (1989, representou um significativo avanço em relação à proteção dos direitos fundamentais de crianças e adolescentes, e originou mudanças paradigmáticas ao responsabilizar adolescentes em conflito com a lei por meio de medidas socioeducativas. Apesar dos avanços observados, a realidade dos adolescentes que cumprem medidas socioeducativas continua marcada por quadro de violação de direitos. Desse modo, pretende-se analisar as graves e recorrentes violações de direitos humanos a adolescentes em conflito com a lei verificadas no estado do Rio Grande do Norte, como territorialidade específica. Compreende-se que, apesar das limitações, acionar o sistema americano de direitos humanos revela-se uma importante ferramenta. Estatuto da Criança e do Adolescente, Sistema Socioeducativo, sistema americano de direitos humanos

  5. CDC STATE System E-Cigarette Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  6. CDC STATE System E-Cigarette Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  7. CDC STATE System Tobacco Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Indoor Air....

  8. Joint Statement on Insect Repellents by EPA and CDC

    Science.gov (United States)

    The EPA and the CDC are recommending the public to use insect repellents and take other precautions to avoid biting insects that carry serious diseases. This statement discusses diseases of concern, government roles, and repellent selection and use.

  9. CDC STATE System E-Cigarette Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Youth Access....

  10. CDC WONDER: Vaccine Adverse Event Reporting System (VAERS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Vaccine Adverse Event Reporting System (VAERS) online database on CDC WONDER provides counts and percentages of adverse event case reports after vaccination, by...

  11. CDC STATE System Tobacco Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Indoor Air. The...

  12. CDC Vital Signs: Preventing Pregnancies in Younger Teens

    Science.gov (United States)

    ... and nurses could use this opportunity to discuss advantages and disadvantages of different contraceptive methods and the ... Director for Communications (OADC) Email Recommend Tweet YouTube Instagram Listen Watch RSS ABOUT About CDC Jobs Funding ...

  13. CDC STATE System E-Cigarette Legislation - Licensure

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Licensure. The...

  14. CDC STATE System E-Cigarette Legislation - Smokefree Campus

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  15. CDC STATE System E-Cigarette Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Youth Access....

  16. CDC STATE System E-Cigarette Legislation - Smokefree Campus

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  17. CDC STATE System E-Cigarette Legislation - Licensure

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Licensure....

  18. CDC STATE System E-Cigarette Legislation - Tax

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Tax. The...

  19. CDC WONDER: Compressed Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CDC WONDER Mortality - Underlying Cause of Death online database is a county-level national mortality and population database spanning the years since 1979...

  20. CDC WONDER: Detailed Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Detailed Mortality - Underlying Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2009. Data are...

  1. CDC STATE System Tobacco Legislation - Smokefree Indoor Air Summary

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Indoor Air. The...

  2. CDC STATE System Tobacco Legislation - Smokefree Indoor Air Summary

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Indoor Air....

  3. CDC STATE System E-Cigarette Legislation - Preemption

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Preemption. The...

  4. CDC STATE System E-Cigarette Legislation - Tax

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Tax. The STATE...

  5. CDC STATE System E-Cigarette Legislation - Preemption

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2017. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Preemption....

  6. CDC WONDER: Daily Air Temperatures and Heat Index

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Daily Air Temperature and Heat Index data available on CDC WONDER are county-level daily average air temperatures and heat index measures spanning the years...

  7. CDC STATE System E-Cigarette Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2018. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  8. CDC Vital Signs: Making Food Safer to Eat

    Science.gov (United States)

    ... where food is grown to cutting boards in kitchens. What we eat and how we eat have ... Sprouts, leafy greens, roots, fish, grains-beans, shellfish, oil-sugar, and dairy. Source: CDC National Outbreak Reporting ...

  9. CDC Vital Signs: Adult Smoking among People with Mental Illness

    Science.gov (United States)

    ... Evidence shows that there has been direct tobacco marketing to people with mental illness and other vulnerable ... Associate Director for Communications (OADC) Email Recommend Tweet YouTube Instagram Listen Watch RSS ABOUT About CDC Jobs ...

  10. CDC Vital Signs–HIV Testing

    Centers for Disease Control (CDC) Podcasts

    2017-11-28

    This podcast is based on the December 2017 CDC Vital Signs report. In the U.S., about 15 percent of people who have HIV don't know they have it. Learn about the importance of testing, early diagnosis, and treatment.  Created: 11/28/2017 by Centers for Disease Control and Prevention (CDC).   Date Released: 11/28/2017.

  11. CDC Vital Signs–African American Health

    Centers for Disease Control (CDC) Podcasts

    2017-05-02

    This podcast is based on the May 2017 CDC Vital Signs report. The life expectancy of African Americans has improved, but it’s still an average of four years less than whites. Learn what can be done so all Americans can have the opportunity to pursue a healthy lifestyle.  Created: 5/2/2017 by Centers for Disease Control and Prevention (CDC).   Date Released: 5/2/2017.

  12. CDC Vital Signs–Preventing Stroke Deaths

    Centers for Disease Control (CDC) Podcasts

    2017-09-06

    This podcast is based on the September 2017 CDC Vital Signs report. Each year, more than 140,000 people die and many survivors face disability. Eighty percent of strokes are preventable. Learn the signs of stroke and how to prevent them.  Created: 9/6/2017 by Centers for Disease Control and Prevention (CDC).   Date Released: 9/6/2017.

  13. CDC Vital Signs–Cancer and Obesity

    Centers for Disease Control (CDC) Podcasts

    2017-10-04

    This podcast is based on the October 2017 CDC Vital Signs report. Obesity is a leading cancer risk factor. Unfortunately, two out of three U.S. adults weigh more than recommended. Find out what can be done to help people get to and keep a healthy weight.  Created: 10/4/2017 by Centers for Disease Control and Prevention (CDC).   Date Released: 10/4/2017.

  14. CDC's Emergency Management Program activities - worldwide, 2003-2012.

    Science.gov (United States)

    2013-09-06

    In 2003, recognizing the increasing frequency and complexity of disease outbreaks and disasters and a greater risk for terrorism, CDC established the Emergency Operations Center (EOC), bringing together CDC staff members who respond to public health emergencies to enhance communication and coordination. To complement the physical EOC environment, CDC implemented the Incident Management System (IMS), a staffing structure and set of standard operational protocols and services to support and monitor CDC program-led responses to complex public health emergencies. The EOC and IMS are key components of CDC's Emergency Management Program (EMP), which applies emergency management principles to public health practice. To enumerate activities conducted by the EMP during 2003-2012, CDC analyzed data from daily reports and activity logs. The results of this analysis determined that, during 2003-2012, the EMP fully activated the EOC and IMS on 55 occasions to support responses to infectious disease outbreaks, natural disasters, national security events (e.g., conventions, presidential addresses, and international summits), mass gatherings (e.g., large sports and social events), and man-made disasters. On 109 other occasions, the EMP was used to support emergency responses that did not require full EOC activation, and the EMP also conducted 30 exercises and drills. This report provides an overview of those 194 EMP activities.

  15. Estudos latino-americanos sobre melancolia: um transtorno do humor melhor definido para o CID-11 Melancholia in Latin American studies: a distinct mood disorder for the ICD-11

    Directory of Open Access Journals (Sweden)

    Mario F. Juruena

    2011-05-01

    Full Text Available OBJETIVO: A depressão melancólica é um diagnóstico psiquiátrico de história de vida, geralmente com episódios recorrentes. Melancolia é uma síndrome com longa duração e características específicas de psicopatologia, insuficientemente diferenciada de depressão maior por um especificador no DSM-IV e parcialmente descrito nos critérios da Classificação Internacional de Doenças-10ª Edição. Dentro da classificação atual, é frequentemente vista em pacientes gravemente doentes com depressão e transtorno bipolar. No entanto, a melancolia possui uma homogeneidade psicopatológica e biológica distinta na experiência clínica e nos marcadores de testes laboratoriais, e é diferencialmente sensível às intervenções terapêuticas específicas. O objetivo deste estudo é revisar a literatura de artigos publicados por autores latino-americanos sobre a melancolia. MÉTODO: Realizou-se busca de artigos latino-americanos de informações relevantes para a revisão da Classificação Internacional de Doenças-10ª Edição de transtornos mentais e comportamentais em pacientes com depressão melancólica. Foi avaliada a qualidade do design de todos os estudos e realizada uma revisão abrangente sobre o assunto, com o objetivo de considerar a contribuição latino-americana para inclusão da melancolia como uma entidade distinta na futura Classificação Internacional de Doenças-11ª Edição. RESULTADOS E CONCLUSÃO: Os estudos latino-americanos fundamentam o diagnóstico da melancolia com uma psicopatologia e psiconeuroendocrinologia própria que fundamentam ser reconhecida como um transtorno de humor identificável e merecedor de uma atenção específica nos sistemas de classificação, como um transtorno de humor distinto, identificável e especificamente tratável.OBJECTIVE: Melancholic depression is a lifetime diagnosis, typically with recurrent episodes. Melancholia, a syndrome with a long history and distinctive

  16. Depletion of cdc-25.3, a Caenorhabditis elegans orthologue of cdc25, increases physiological germline apoptosis.

    Science.gov (United States)

    Sung, Minhee; Kawasaki, Ichiro; Shim, Yhong-Hee

    2017-07-01

    In Caenorhabditis elegans hermaphrodites, physiological germline apoptosis is higher in cdc-25.3 mutants than in wild-type. The elevated germline apoptosis in cdc-25.3 mutants seems to be induced by accumulation of double-stranded DNA breaks (DSBs). Both DNA damage and synapsis checkpoint genes are required to increase the germline apoptosis. Notably, the number of germ cells that lose P-granule components, PGL-1 and PGL-3, increase in cdc-25.3 mutants, and the increase in germline apoptosis requires the activity of SIR-2.1, a Sirtuin orthologue. These results suggest that elevation of germline apoptosis in cdc-25.3 mutants is induced by accumulation of DSBs, leading to a loss of PGL-1 and PGL-3 in germ cells, which promotes cytoplasmic translocation of SIR-2.1, and finally activates the core apoptotic machinery. © 2017 Federation of European Biochemical Societies.

  17. Role of AtCDC48 & the AtCDC48 Regulatory Protein Family, PUX, in Plant Cell Morphogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Bednarek, Sebastian, Y.

    2009-11-08

    The long-term objective of this work is to understand the molecular events and mechanisms involved in secretory membrane trafficking and organelle biogenesis, which are crucial for normal plant growth and development. Our studies have suggested a vital role for the cytosolic chaperone Cdc48p/p97 during cytokinesis and cell expansion which are highly dependent upon secretory membrane trafficking. Localization studies have shown that the plant Cdc48p/p97, AtCDC48, and the Arabidopsis ortholog of the ER- and Golgi-associated SNARE, syntaxin 5, (referred to as SYP31) are targeted to the division plane during cytokinesis. In addition, AtCDC48 and SYP31 were shown to interact in vitro and in vivo. To characterize further the function of AtCDC48 and SYP31 we have utilized affinity chromatography and MALDI-MS to identify several plant-specific proteins that interact with SYP31 and/or modulate the activity of AtCDC48 including two UBX (i.e. ubiquitin-like) domain containing proteins, PUX1 and PUX2 (Proteins containing UBX domain). These proteins define a plant protein family consisting of 15 uncharacterized members that we postulate interact with AtCDC48. Biochemical studies have demonstrated that PUX2 is a novel membrane adapter for AtCDC48 that mediates AtCDC48/SYP31 interaction and is likely to control AtCDC48-dependent membrane fusion. In contrast, PUX1 negatively regulates AtCDC48 by inhibiting its ATPase activity and by promoting the disassembly of the active hexamer. These findings provide the first evidence that the assembly and disassembly of the CDC48/p97complex is actually a dynamic process. This new unexpected level of regulation for CDC48/p97 was demonstrated to be critical in vivo as pux1 loss-of-function mutants grow faster than wild-type plants. These studies suggest a role for AtCDC48 in plant cell cycle progression including cytokinesis and/or cell expansion. The proposed studies are designed to: 1) characterize further the localization and function of AtCDC

  18. O marxismo indo-americano de Mariátegui e a polêmica com a III Internacional Comunista.

    Directory of Open Access Journals (Sweden)

    María Gabriela Guillén

    2012-05-01

    Full Text Available Partindo da perspectiva marxista indo-americana de José Carlos Mariátegui, o artigo visa a exposição de fatores teóricos e históricos que culminaram na polêmica com a III Internacional Comunista na América Latina. A problemática evidencia a originalidade do pensamento de Mariátegui, cujo eixo central é a interpretação dos fatos históricos, políticos e sociais latino-americanos sob a perspectiva dialética do desenvolvimento desigual e combinado para explicar a dinâmica particular da realidade peruana.

  19. Órgãos genitais femininos do Lobo-marinho-sul-americano (Arctocephalus australis): uma abordagem morfofuncional

    OpenAIRE

    Alex Sander Dias Machado

    2009-01-01

    O Lobo-marinho-sul-americano (A. australis) apresenta particularidades em seu ciclo reprodutivo que revelam sua interação com o ecossistema onde habita. Dentre estas podemos citar o intervalo entre partos de 12 meses, a sincronização dos partos e cópulas no início do verão, umlongo período de diapausa e uma implantação do blastocisto no inicio do inverno, que ocorre 4 a 5 meses após a cópula. A anatomia e fisiologia reprodutivas desta espécie ainda não foram profundamente estudadas. O present...

  20. Poder civil y derechos naturales de los indios americanos según Fray Alonso de la Veracruz

    Directory of Open Access Journals (Sweden)

    Manuel Méndez Alonzo

    2013-01-01

    Full Text Available El presente trabajo investiga las tesis sobre el poder civil de Alonso de la Veracruz que buscan incorporar en la comunidad política española a los habitantes autóctonos del Nuevo Mundo, tesis que suelen relacionarse con F. de Vitoria y el tomismo español, y que últimamente son consideradas parte del republicanismo novohispano elaborado desde la periferia americana. Se busca demostrar que su propósito era aplicar una teoría de derechos naturales, sin que ello implique participación política de los indios americanos. Se analiza la postura del fraile frente a la diversidad cultural y la guerra contra los indios.

  1. O terror e a mídia: o neoconservadorismo norte-americano e o islã radical

    OpenAIRE

    Ortunes, Leandro

    2013-01-01

    Esta dissertação tem como objeto de estudo a construção da imagem do neoconservadorismo norte-americano e do Islã radical na mídia. Através de uma fundamentação teórica sobre os choques culturais e os confrontos armados entre Ocidente e Oriente, mais precisamente entre Estados Unidos e países com grande atuação islâmica na forma de governo; apresentaremos os pontos de divergência e possíveis similaridades destes dois grupos. Com esta fundamentação, observaremos as divergências e as superficia...

  2. Qualidade de vida e reformas de programas sociais: o Brasil no cenário latino-americano

    OpenAIRE

    Draibe,Sônia Miriam

    1993-01-01

    Com base na constatação de que o Brasil ocupa um dos piores lugares na América Latina no tocante à eficácia de suas políticas sociais, mesmo comparado com países que não promoveram a reforma dos seus programas sociais sob regime democrático (como o Chile) a autora busca caraterizar a especificidade do caso brasileiro no cenário latino-americano. O pano de fundo da sua análise é dado pelos problemas da reforma do Estado e dos caminhos da modernização do país.

  3. Decreased uv mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae

    International Nuclear Information System (INIS)

    Prakash, L.; Hinkle, D.; Prakash, S.

    1978-01-01

    A DNA replication mutant of yeast, cdc8, was found to decrease uv-induced reversion of lys2-1, arg4-17, tryl and ural. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following uv irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since uv-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme

  4. Decreased uv mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae

    Energy Technology Data Exchange (ETDEWEB)

    Prakash, L.; Hinkle, D.; Prakash, S.

    1978-01-01

    A DNA replication mutant of yeast, cdc8, was found to decrease uv-induced reversion of lys2-1, arg4-17, tryl and ural. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following uv irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since uv-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.

  5. Inovações financeiras e institucionais do sistema de financiamento residencial americano Institutional and financial innovations in the U.S. housing finance system

    Directory of Open Access Journals (Sweden)

    Rafael Fagundes Cagnin

    2009-09-01

    Full Text Available This article has the gold of summarize the institutional modifications of the US housing finance system between the 60's and the end of the 90's. Those changes narrowed the bonds between this segment of the credit market and the securitized financial markets, encouraging the foundation and diffusion of financial innovations that are in the core of the current financial crises.

  6. OS DIREITOS HUMANOS E O CONSTITUCIONALISMO INTERNACIONAL LATINO-AMERICANO -- HUMAN RIGHTS AND THE LATIN AMERICAN INTERNATIONAL CONSTITUTIONALISM

    Directory of Open Access Journals (Sweden)

    Feliciano de Carvalho

    2016-06-01

    Full Text Available Resumo: Os países da América Latina estão regidos por uma ordem constitucional comum, especificamente a que decorre dos tratados internacionais sobre direitos humanos. Com efeito, conforme a tendência do bloco de constitucionalidade, além das respectivas constituições, vários países latino-americanos conferem aos tratados internacionais a mesma hierarquia das normas constitucionais quando têm por objeto os direitos humanos. A relevância da pesquisa decorre em razão de a supremacia constitucional ir além das prescrições constitucionais para as normas internacionais que promovem os direitos humanos. Aborda-se o conceito material de direitos humanos a partir da carga valorativa dos bens jurídicos mais relevantes para a sociedade, em especial, os valores da dignidade humana, vida, liberdade e igualdade, de modo que os direitos humanos seriam os daí imediatamente decorrentes. Analisa-se a tendência dos Estados latino-americanos de respeitarem os tratados internacionais sobre direitos humanos como se fossem normas constitucionais, com especial atenção a respeito da experiência brasileira. A pesquisa é bibliográfica, descritiva, e tem-se a finalidade de aprimorar o conhecimento sobre o tema. Ao final, conclui-se que surge na América Latina a primeira ideia de uma Constituição Internacional Comunitária, em face da prevalência dos direitos humanos no cenário supranacional. Palavras-chave: Direitos Humanos. Constitucionalismo. Bloco. América Latina.

  7. Sistema detector d'incendis WSN

    OpenAIRE

    Domínguez López, Emiliano

    2012-01-01

    Sistema detector d'incendis aprofitant una xarxa de sensors sense fils (WSN) mitjançant un sistema encastat. Sistema detector de incendios aprovechando una red de sensores inalámbricos (WSN) mediante un sistema empotrado.

  8. SISTEMA DE PRESION NEGATIVA

    OpenAIRE

    Olavarría-Beivide, Encarnación; Herrero-Montes, Manuel; Bravo, Paula Paras

    2014-01-01

    EVENTRACIÓN ABDOMINAL, SISTEMA DE VACÍO Y CALIDAD DE VIDA. INTRODUCCION Varón, 40 años que ingresa en Cirugía para eventroplastia abdominal con prótesis. Tras dehiscencia de herida, presenta exudado profuso. Tratada con cura humeda tradicional, tras ineficacia y mal control del exudado, se decide la aplicación de sistema de presión negativa (SPN) OBJETIVOS Restablecer la integridad cutáneaValorar los beneficios del SPN METODOLOGIA Establecer diagnosticos, objetivos e interve...

  9. Sistema de seguridad activa

    OpenAIRE

    Yaure Machuca, Jhonny Felipe

    2007-01-01

    El trabajo aquí realizado responde a las necesidades que se han dado sobre la falta de información de las cualidades de seguridad activa que prestan los vehículos al momento de circular. Dentro del Capítulo 1, se desarrolló una breve reseña de la historia del nacimiento e implementación de la seguridad activa en los automóviles, la misión que tiene este sistema dentro del vehículo, y como se lo define a este sistema. Dentro del Capítulo 2, se comienza describiendo los componentes que co...

  10. El Sistema inspirerede projekter

    DEFF Research Database (Denmark)

    Holst, Finn; Chemi, Tatiana

    Rapporten er resultat af pilotprojektet ”Forundersøgelse El Sistema DK” gennemført af Ph.D. Finn Holst (DPU, Aarhus Universitet) og Ph.D. Tatiana Chemi (Aalborg Universitet) i 2015. I rapporten indgår desuden bidrag fra Sofie Holm, Sara Fink Søndergaard og Stine Møgelbjerg Johansen. Pilotprojektet...... er gennemført for DMKLs El Sistema-udvalg med støtte fra Statens Kunstfond, Projektstøtteudvalget for Musik....

  11. El sistema constructivo

    Directory of Open Access Journals (Sweden)

    Leser S., Heinz

    2003-06-01

    Full Text Available Se describe el sistema constructivo de entramado en madera, “plataforma” (platform frame utilizado preferentemente en las construcciones de varios pisos en el campamento minero de Sewell, Chile./The following is a description of the platform frame used in several stories buildings in the mining settlement Sewell, in Chile.

  12. El Sistema's Open Secrets

    Science.gov (United States)

    Booth, Eric

    2011-01-01

    In this article, the author talks about Venezuela's national youth orchestra program called El Sistema whose attributes offer a direct challenge to traditional Western music practices. As U.S. classical music--and all "high arts"--struggle to find relevance to more than the small "arts club" percentage of the U.S. populace, El…

  13. Informe Signos Vitales de los CDC Obesidad infantil - (Childhood Obesity)

    Centers for Disease Control (CDC) Podcasts

    2013-08-06

    Este podcast se basa en el informe Signos Vitales de los CDC de agosto del 2013. La tasa de obesidad entre los niños en edad prescolar de bajos ingresos ha disminuido, pero todavía uno de cada seis niños hispanos es obeso. Este programa habla brevemente sobre lo que se puede hacer.  Created: 8/6/2013 by Centers for Disease Control and Prevention (CDC).   Date Released: 8/6/2013.

  14. Rankings acadêmicos na educação superior: mapeamento da sua expansão no espaço ibero-americano

    Directory of Open Access Journals (Sweden)

    Henrique da Silva Lourenço

    2015-05-01

    Full Text Available Neste artigo explora-se o universo dos rankings acadêmicos para mapear a sua expansão no espaço ibero-americano, tendo como foco principal os rankings promovidos pelo setor privado, principalmente pelo mercado editorial. Foi realizada pesquisa exploratória por meio de análises documentais e bibliográficas tomando como referência dados disseminados na Internet. A pesquisa revela a liderança assumida pela Espanha em termos de produção de rankings internacionais, com visível impacto regional, bem como a existência de três países latino-americanos (Chile, Brasil e México que se caracterizam por terem em suas realidades nacionais rankings acadêmicos com trajetória e tradição bem definidas.

  15. Passado, presente e futuro dos princípios contábeis norte-americanos - US GAAP Lições para contabilidade brasileira

    Directory of Open Access Journals (Sweden)

    João Henrique Pederiva

    2009-10-01

    Full Text Available Como se estruturam as normas contábeis adotadas para registrar as atividades econômicas norte-americanas - United States Generally Accepted Accounting Principles - US GAAP (Princípios Contábeis Geralmente Aceitos Norte Americanos, quais os seus principais eixos de desenvolvimento doutrinário e que interesse há nesse conhecimento para a contabilidade brasileira? A relevância de obter respostas a esses questionamentos resulta da importância econômica dos Estados Unidos da America para as demais nações. Afinal, os recentes problemas nos mercados de capitais norte-americanos comprometeram, em dois anos e meio, US$ 7,7 trilhões da poupança destinada a aposentadoria dos trabalhadores daquele país. Atente-se que muitos países, inclusive o Brasil, buscam recursos nesses mercados de capitais.

  16. CDC Vital Signs–Arthritis in America

    Centers for Disease Control (CDC) Podcasts

    2017-03-07

    This podcast is based on the March 2017 CDC Vital Signs report. Many adults in the United States have arthritis. Learn how to reduce the pain of arthritis, as well as manage the condition.  Created: 3/7/2017 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 3/7/2017.

  17. Child Passenger Safety (A Cup of Health with CDC)

    Centers for Disease Control (CDC) Podcasts

    2016-09-29

    Proper installation and use of car seats and booster seats for child passengers can save their lives. CDC recommends drivers ensure children are always buckled up. In this podcast, Bethany West discusses how to keep young passengers as safe as possible.  Created: 9/29/2016 by MMWR.   Date Released: 9/29/2016.

  18. Dangerous Creatures - A Visit to the CDC Insectary

    Centers for Disease Control (CDC) Podcasts

    2012-11-07

    Tour CDC’s insectary with Sofi, a young host, and learn from CDC researchers about mosquitoes and insecticide resistance.  Created: 11/7/2012 by Center for Global Health (CGH).   Date Released: 12/20/2012.

  19. Cdc20 control of cell fate during prolonged mitotic arrest

    DEFF Research Database (Denmark)

    Nilsson, Jakob

    2011-01-01

    The fate of cells arrested in mitosis by antimitotic compounds is complex but is influenced by competition between pathways promoting cell death and pathways promoting mitotic exit. As components of both of these pathways are regulated by Cdc20-dependent degradation, I hypothesize that variations...

  20. CDC 24/7: Saving Lives, Protecting People

    Centers for Disease Control (CDC) Podcasts

    2012-06-04

    24/7, CDC provides health information, responds to public health emergencies and natural disasters, and monitors disease.  Created: 6/4/2012 by Office of the Associate Director of Communciation (OADC).   Date Released: 6/4/2012.

  1. "A wild and wondrous ride": CDC field epidemiologists in the east Pakistan smallpox and cholera epidemics of 1958 "Uma louca e maravilhosa jornada": epidemiologistas de campo do CDC nas epidemias de varíola e cólera do Paquistão Oriental em 1958

    Directory of Open Access Journals (Sweden)

    Paul Greenough

    2011-02-01

    desenvolvimento. Langmuir descreveu o episódio como uma "jornada louca e maravilhosa", porém pareceu mais um pesadelo: o departamento de saúde do Paquistão Oriental entrou em colapso; um movimento popular assumiu a vacinação e desperdiçou suprimentos de vacinas; jornalistas hostis questionaram as motivações ocultas dos americanos; e iniciou-se uma rivalidade profissional entre americanos e o epidemiologista britânico Aidan Cockburn. No final da epidemia, em julho de 1958, tinham sido vacinados trinta milhões de bengalis contra varíola, mas outros vinte mil sucumbiram. Esse episódio foi a primeira intervenção sistemática do CDC no exterior.

  2. experiencias en el sistema

    Directory of Open Access Journals (Sweden)

    B. Fernández Manjón

    2005-01-01

    Full Text Available En este trabajo se presenta la experiencia de aplicación e implementación del modelo de objetos educativos en el sistema . Se describe una visión práctica de la aplicación de las tecnologías de marcado tanto en la estructuración de los cursos como en el desarrollo de contenidos que pueden ser adaptados al usuario. En se han seguido las especificaciones de IMS, desarrollándose un soporte completo del empaquetamiento de cursos (content packaging reflejado en un sistema de autoría de cursos y una herramienta de importación. Además se ha particularizado un perfil específico de cursos y de objetos educativos que permiten su adaptación al usuario.

  3. Sistemas integrados con Arduino

    OpenAIRE

    EL YAKOUTI, MOHAMMED

    2017-01-01

    Design of a robot prototype remotely controllable from Bluetooth using Arduino. Control and testing of sensors and events interacting with Arduino and Bluetooth. Diseño de un prototipo de robot controlable remotamente con Bluetooth utilizando Arduino. Control y verificación de los sensores y eventos que interactúan mediante el Arduino y el Bluetooth. El Yakouti, M. (2017). Sistemas integrados con Arduino. http://hdl.handle.net/10251/89274. TFGM

  4. SISTEMA DE MONITORAMENTO AUTOMATIZADO

    Directory of Open Access Journals (Sweden)

    Raiane viana Souza

    2016-11-01

    Full Text Available Atualmente existe uma grande necessidade de ter sistemas que auxiliem no monitoramento de ambientes, por exemplo: uma residência; permitindo assim que o usuário possa controlar e ter acesso a tudo que acontece quando o mesmo estiver ausente. Este projeto propõe realizar o desenvolvimento de um sistema de monitoramento automatizado, cujo objetivo é identificar a presença de pessoas e capturar temperaturas de um ambiente. O sistema é composto por um microcontrolador Arduíno, no qual estará acoplado: uma Webcam, sensores de presença e temperatura e um módulo wifi, que possibilitará o envio dos dados capturados ao servidor web. No protótipo desenvolvido foram implantados todos os recursos definidos com base nos requisitos do usuário, dentre os quais podem-se destacar: permitir detectar a presença de pessoas em ambiente, capturar imagens, capturar temperatura em um intervalo de tempo específico e enviar todos os dados para o servidor; permitir que o usuário visualize as informações capturadas através de um site; gerar relatórios e gráficos com as informações capturadas e permitir que o usuário exporte as informações em formato de arquivo.

  5. O folclore está no ar: a performance sônica da música folclórica brasileira no projeto pan-americano

    Directory of Open Access Journals (Sweden)

    Rafael Henrique Soares Velloso

    2016-08-01

    Full Text Available Neste artigo busco discutir a relação entre a produção de uma nova paisagem sonora pela radiodifusão comercial associada aos projetos culturais pan-americanos e as atividades de uma rede constituída por produtores musicais, pesquisadores, compositores e políticos, por meio da análise da representação da música brasileira veiculada em programas produzidos por Alan Lomax na rádio norte-americana CBS. Considerando tais programas como forma de discutir o papel desta música no projeto Pan-Americano, intento identificar nessas produções a emergência de uma nova sensibilidade musical sobre o folclore musical sul-americano como resultado do intercâmbio entre especialistas no assunto no Brasil e EUA. Esta proposta de análise visa, igualmente, contribuir para a história da música popular brasileira, propondo a desconstrução da noção de autenticidade brasileira associada ao folclore na radiodifusão comercial como elemento central na construção de sua identidade. 

  6. Dieta del Cernícalo Americano (Falco sparverius Linnaeus, 1758 en dos hábitats interandinos del norte de Ecuador

    Directory of Open Access Journals (Sweden)

    Glenda M. Pozo-Zamora

    2017-07-01

    Full Text Available El cernícalo americano es una especie de ave rapaz ampliamente distribuida desde Alaska hasta Tierra de Fuego en Argentina. Su dieta generalista está basada principalmente en invertebrados. En Ecuador existe escasa información biológica de ésta especie, desconociéndose aspectos de alimentación. En el presente trabajo se da a conocer la dieta del cernícalo americano, por medio del análisis de egagrópilas colectadas en dos hábitats ubicados en la región interandina en el norte de Ecuador. Los ítems más representativos para Sangolquí y Tababela fueron los coleópteros (48.4 y 39.5%, seguidos por orthópteros (31.3 y 30.7 % respectivamente. En términos de biomasa, los mamíferos son los más importantes dentro de la dieta del cernícalo americano con el 40.3% para Sangolquí y el 70.8% en Tababela. El índice de Shannon (H´= 1.894 indica una mediana diversidad de presas, y la amplitud de dieta (0.26 indica que ésta especie presenta una dieta especialista, difiriendo de otros hábitats donde presenta dieta generalista.

  7. Cdc42-dependent structural development of auditory supporting cells is required for wound healing at adulthood

    DEFF Research Database (Denmark)

    Anttonen, Tommi; Kirjavainen, Anna; Belevich, Ilya

    2012-01-01

    Cdc42 regulates the initial establishment of cytoskeletal and junctional structures, but only little is known about its role at later stages of cellular differentiation. We studied Cdc42's role in vivo in auditory supporting cells, epithelial cells with high structural complexity. Cdc42 inactivat......, a process that is critical to scar formation. Thus, Cdc42 is required for structural differentiation of auditory supporting cells and this proper maturation is necessary for wound healing in adults....

  8. Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus.

    Science.gov (United States)

    De Felice, Mariarita; Esposito, Luca; Rossi, Mosè; Pisani, Francesca M

    2006-02-01

    The biological role of archaeal proteins, homologous to the eukaryal replication initiation factors of cell division control (Cdc6) and origin recognition complex (ORC1), has not yet been clearly established. The hyperthermophilic crenarchaeon Sulfolobus solfataricus (referred to as Sso) possesses three Cdc6/ORC1-like factors, which are named Sso Cdc6-1, Cdc6-2 and Cdc6-3. This study is a report on the biochemical characterization of Sso Cdc6-1 and Cdc6-3. It has been found that either Sso Cdc6-1 or Cdc6-3 behave as monomers in solutions by gel filtration analyses. Both factors are able to bind to various single-stranded and double-stranded DNA ligands, but Sso Cdc6-3 shows a higher DNA-binding affinity. It has also been observed that either Sso Cdc6-1 or Cdc6-3 inhibit the DNA unwinding activity of the S. solfataricus homo-hexameric mini-chromosome maintenance (MCM)-like DNA helicase (Sso MCM); although they strongly stimulate the interaction of the Sso MCM with bubble-containing synthetic oligonucleotides. The study has also showed, with surface plasmon resonance measurements, that Sso Cdc6-2 physically interacts with either Sso Cdc6-1 or Sso Cdc6-3. These findings may provide important clues needed to understand the biological role that is played by each of these three Cdc6 factors during the DNA replication initiation process in the S. solfataricus cells.

  9. Economia e instituições importam? Um estudo do comportamento eleitoral em países latino-americanos

    Directory of Open Access Journals (Sweden)

    Jaqueline da Silva Borges

    2016-12-01

    Full Text Available Os estudos de comportamentos eleitorais contemporâneos, para além de entender a relevância de aspectos econômicos, têm incorporado em suas análises as variáveis de caráter institucional. De acordo com esse aporte teórico, é preciso atentar para o contexto político em que os eleitores estão inseridos. Partindo de tais preceitos, o presente trabalho tem por objetivo analisar a relevância de aspectos econômicos e institucionais no comportamento eleitoral de dezoito países latino-americanos entre 2008 e 2012. Assim, mensura-se em que medida as variáveis institucionais se mostram relevantes no vínculo que o eleitor estabelece entre desempenho de governo na economia e a avaliação dada ao governante. Os principais achados reforçam, em parte, a pertinência de variáveis institucionais no voto econômico.

  10. NIDIFICACIÓN Y EFICIENCIA REPRODUCTIVA DEL COCODRILO AMERICANO EN UNA RESERVA ECOLÓGICA DE MANATÍ

    Directory of Open Access Journals (Sweden)

    Leticia Torres Martínez

    2013-08-01

    Full Text Available En el presente trabajo se caracteriza la variación espacial y temporal de los patrones reproductivos del cocodrilo americano (Crocodylus acutus Cuvier, entre 2008 y 2010, para las localidades de La Isleta y Los Coquitos, en la Reserva Ecológica Bahía de Nuevas Grandes-La Isleta en Manatí, Las Tunas. Se describen las conductas de nidificación, desove y atención al nido. Además, se analiza la dinámica de las puestas, eclosiones y la morfometría de los huevos. Se detectó que las hembras no despliegan la misma conducta para la construcción del nido, pero en todos los casos la nidificación implica la transformación del paisaje. En ambas localidades las curvas de acumulación de puestas y eclosiones se dividen en tres etapas: inicial, aceleración y meseta. En general, existe consistencia de la correlación entre el largo y ancho de los huevos con tendencia a que los huevos largos y pesados incrementen su natalidad y viabilidad. En La Isleta la altura y tipo de suelo aseguran una elevada eficiencia de reproducción, lo que sugiere mayor estabilidad temporal de factores bióticos y abióticos.

  11. Las antiguas colecciones arqueológicas de Yucatán en el Museo Americano de Historia Natural

    Directory of Open Access Journals (Sweden)

    Adam T. Sellen

    2009-01-01

    Full Text Available Las investigaciones que hemos desarrollado acerca de la historia de las colecciones yucatecas que forman parte del Museo Americano de Historia Natural de Nueva York nos permiten trazar un perfil general de los intereses intelectuales y de algunas de las primeras exploraciones emprendidas en la región. La información asociada a las piezas arqueológicas que actualmente se resguardan en dicho museo nos remite a los trabajos precursores llevados a cabo por reconocidos viajeros como Charnay, Stephens, Le Plongeon o Maler, y también de otros menos conocidos, como los coleccionistas locales. En conjunto, estos protoarqueólogos enfocaron sus esfuerzos a construir el conocimiento de la antigua civilización maya a través de sus evidencias materiales.Our research on the history of Yucatec collections that are now in the American Museum of Natural History in New York allows us to outline a general profile of the intellectual interests and some of the first explorations in the region. The information associated with the archaeological objects that are today held in this museum speak of the early archaeological work carried out by well known explorers such as Charnay, Stephens, Le Plongeon or Maler, and also by lesser-known players such as the local collectors. Together, these proto-archaeologists focused their efforts on constructing knowledge of the ancient Maya civilization through material evidence.

  12. Estrangeirização de Terras na Perspectiva das Formas de Colonialidade no Agro Latino-Americano

    Directory of Open Access Journals (Sweden)

    Luís Felipe Perdigão de Castro

    2017-08-01

    “Acaparamiento de Tierras” y Formas de Colonialidad em el “Agro” LatinoAmericano Resumen La demanda global por tierras se ha convertido en un fenómeno global, dando impulso a lo que se denomina convencionalmente “land grabbing”, “acaparamiento de tierras” ou “estrangeirização de terras”. Sobre la base de este tema, este artículo describe cómo el control de los gobiernos y corporaciones internacionales sobre las tierras y territorios de las comunidades tradicionales muestran la extranjerización de la tierra como una forma reciente de la "continuidad de las formas coloniales de dominación" (Grosfoguel, 2008, p.126. El objetivo es poner en contexto la llamada fiebre por tierras a nivel mundial a través de datos de la FAO (2011, ya la luz de los aspectos sociológicos y jurídicos relacionados. El fondo es para discutir el tema de las interfaces con las formas de la continuidad del colonialismo. Palabras clave: Tierra Estrangeirização; América Latina; Tierras y territorios; Identidades sociales

  13. Sistemas Silvipastoris Silvipastoral Systems

    Directory of Open Access Journals (Sweden)

    Fernando Salgado Bernardino

    2010-02-01

    Full Text Available

    Apesar das pesquisas com sistemas silvipastoris terem sido iniciadas no final da década de 1970, as informações geradas até hoje não são em grande número. As vantagens advindas de um sistema silvipastoril são inúmeras e devidamente reconhecidas. Pelo fato de ser uma técnica de uso da terra capaz de recuperar ecossistemas alterados pelo mau manejo, estes sistemas, pela integração de atividades agrícolas, pecuárias e silviculturais, passam a representar uma tecnologia que confere maior sustentabilidade que os sistemas tradicionais, nos quais os monocultivos são predominantes. Nos últimos anos, instituições de ensino, pesquisa e extensão do País têm se voltado para o desenvolvimento de tais sistemas. O governo de Minas Gerais, por meio de sua Secretaria de Estado de Agricultura e de órgãos vinculados, vem promovendo a condução de modelos agrossilvipastoris num processo integrado de ocupação do solo, denominado lavoura-pecuária-silvicultura. Apesar dos grandes avanços no conhecimento de culturas anuais e dos componentes arbóreos, principalmente o eucalipto, e de gramíneas forrageiras tolerantes à diminuição da intensidade luminosa, ainda é necessário se comnhecer melhor o manejo do sub-bosque sob os efeitos do pastejo.

     

    doi: 10.4336/2009.pfb.60.77

    Although researches related to silvopastoral systems have been initiated many years ago in Brazil, there are few informations generated until now. The advantages from a silvopastoral system are inumerous and well recognized. By the fact of being a technique of land using capable of recovering several disturbed ecosystems, the silvopastoral systems by the integration of agricultural, cattle, and silvicultural activities represent a technology that can achieve higher sustainability than the traditional systems as the monocultures. Today, research centers in Brazil dedicate great attention to agrossilvopastoral systems. The government of Minas Gerais

  14. Caffeine stabilizes Cdc25 independently of Rad3 in S chizosaccharomyces pombe contributing to checkpoint override

    Science.gov (United States)

    Alao, John P; Sjölander, Johanna J; Baar, Juliane; Özbaki-Yagan, Nejla; Kakoschky, Bianca; Sunnerhagen, Per

    2014-01-01

    Cdc25 is required for Cdc2 dephosphorylation and is thus essential for cell cycle progression. Checkpoint activation requires dual inhibition of Cdc25 and Cdc2 in a Rad3-dependent manner. Caffeine is believed to override activation of the replication and DNA damage checkpoints by inhibiting Rad3-related proteins in both S chizosaccharomyces pombe and mammalian cells. In this study, we have investigated the impact of caffeine on Cdc25 stability, cell cycle progression and checkpoint override. Caffeine induced Cdc25 accumulation in S . pombe independently of Rad3. Caffeine delayed cell cycle progression under normal conditions but advanced mitosis in cells treated with replication inhibitors and DNA-damaging agents. In the absence of Cdc25, caffeine inhibited cell cycle progression even in the presence of hydroxyurea or phleomycin. Caffeine induces Cdc25 accumulation in S . pombe by suppressing its degradation independently of Rad3. The induction of Cdc25 accumulation was not associated with accelerated progression through mitosis, but rather with delayed progression through cytokinesis. Caffeine-induced Cdc25 accumulation appears to underlie its ability to override cell cycle checkpoints. The impact of Cdc25 accumulation on cell cycle progression is attenuated by Srk1 and Mad2. Together our findings suggest that caffeine overrides checkpoint enforcement by inducing the inappropriate nuclear localization of Cdc25. PMID:24666325

  15. 75 FR 62844 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2010-10-13

    ... HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD... CDC. Matters to be Discussed: The ACD, CDC will receive updates from the Global Workgroup; State... for More Information: Carmen Villar, M.S.W., Designated Federal Officer, ACD, CDC, 1600 Clifton Road...

  16. SCFCyclin F-dependent degradation of CDC6 suppresses DNA re-replication

    DEFF Research Database (Denmark)

    Walter, David; Hoffmann, Saskia; Komseli, Eirini-Stavroula

    2016-01-01

    origin licensing, however, it is poorly understood how CDC6 activity is constrained in higher eukaryotes. Here we report that the SCF(Cyclin F) ubiquitin ligase complex prevents DNA re-replication by targeting CDC6 for proteasomal degradation late in the cell cycle. We show that CDC6 and Cyclin F...... interact through defined sequence motifs that promote CDC6 ubiquitylation and degradation. Absence of Cyclin F or expression of a stable mutant of CDC6 promotes re-replication and genome instability in cells lacking the CDT1 inhibitor Geminin. Together, our work reveals a novel SCF(Cyclin F...

  17. CDC Vital Signs-Hospital Actions Affect Breastfeeding

    Centers for Disease Control (CDC) Podcasts

    2015-10-06

    This podcast is based on the October 2015 CDC Vital Signs report. Hospitals can implement the Ten Steps to Successful Breastfeeding to be designated as "Baby-Friendly" and support more moms in a decision to breastfeed.  Created: 10/6/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/6/2015.

  18. CDC Vital Signs-Safer Food Saves Lives

    Centers for Disease Control (CDC) Podcasts

    2015-11-03

    This podcast is based on the November 2015 CDC Vital Signs report. Contaminated food sent to several states can cause multistate outbreaks of foodborne illness and make a lot of people seriously ill. Learn what can be done to prevent and stop outbreaks.  Created: 11/3/2015 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 11/3/2015.

  19. CDC Vital Signs-Protect Patients from Antibiotic Resistance

    Centers for Disease Control (CDC) Podcasts

    2016-03-03

    This podcast is based on the March 2016 CDC Vital Signs report. Patients can get serious healthcare-associated infections, or HAIs, while receiving medical treatment in a healthcare facility. Learn how to prevent healthcare-associated infections.  Created: 3/3/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 3/3/2016.

  20. CDC Vital Signs-Communication Can Save Lives

    Centers for Disease Control (CDC) Podcasts

    2015-08-04

    This podcast is based on the August 2015 CDC Vital Signs report. Antibiotic-resistant germs cause at least 23,000 deaths each year. Learn how public health authorities and health care facilities can work together to save lives.  Created: 8/4/2015 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 8/4/2015.

  1. Sistema seguidor solar microcontrolado

    Directory of Open Access Journals (Sweden)

    Daiane Maria Mendes Duarte

    2015-12-01

    Full Text Available A demanda de energia elétrica é cada vez maior devido ao grande crescimento da população e do novo estilo de vida adotado pela sociedade moderna, cada vez mais industrializada. Nesse sentido, o consumo de energia vem apresentando um crescimento acelerado. Concomitantemente, a sociedade se movimenta no sentido de se conscientizar das alterações ocasionadas na natureza, e, assim, surge a necessidade de novos meios de geração de energia, menos impactantes ao meio ambiente, denominadas energias renováveis. Este artigo vem apresentar um sistema seguidor solar microcontrolado, que possibilita uma maior captação de energia em placas solares, pois as posiciona sempre com sua face voltada para o sol. O sistema de controle é baseado nas equações matemáticas cujos resultados são as posições do sol num dado dia e em uma dada hora. Estas coordenadas são calculadas e resultam no deslocamento da célula fotovoltaica por meio de um motor.

  2. Science in Emergency Response at CDC: Structure and Functions.

    Science.gov (United States)

    Iskander, John; Rose, Dale A; Ghiya, Neelam D

    2017-09-01

    Recent high-profile activations of the US Centers for Disease Control and Prevention (CDC) Emergency Operations Center (EOC) include responses to the West African Ebola and Zika virus epidemics. Within the EOC, emergency responses are organized according to the Incident Management System, which provides a standardized structure and chain of command, regardless of whether the EOC activation occurs in response to an outbreak, natural disaster, or other type of public health emergency. By embedding key scientific roles, such as the associate director for science, and functions within a Scientific Response Section, the current CDC emergency response structure ensures that both urgent and important science issues receive needed attention. Key functions during emergency responses include internal coordination of scientific work, data management, information dissemination, and scientific publication. We describe a case example involving the ongoing Zika virus response that demonstrates how the scientific response structure can be used to rapidly produce high-quality science needed to answer urgent public health questions and guide policy. Within the context of emergency response, longer-term priorities at CDC include both streamlining administrative requirements and funding mechanisms for scientific research.

  3. Projecte sistema de control FIA

    OpenAIRE

    Simonet Ramon, Guillermo

    2015-01-01

    Disseny d'un sistema de control de les diverses competicions que organitza la FIA (Federació Internacional d'Automobilisme). Diseño de un sistema de control de las diversas competiciones que organiza la FIA (Federación Internacional de Automovilismo). Master thesis for the Computer Science program.

  4. Sistemas de Identidade Visual Corporativa

    OpenAIRE

    Raposo, D.

    2009-01-01

    Este artigo trata de fazer um pequeno percurso histórico sobre como a marca se converteu nos sistemas de identidade visual corporativa. Alguns dos principais projectos de identidade ajudam a entender que motivações originaram a necessidade de organização dos sistemas de identidade.

  5. Signaling Cascades Governing Cdc42-Mediated Chondrogenic Differentiation and Mensenchymal Condensation.

    Science.gov (United States)

    Wang, Jirong R; Wang, Chaojun J; Xu, Chengyun Y; Wu, Xiaokai K; Hong, Dun; Shi, Wei; Gong, Ying; Chen, Haixiao X; Long, Fanxin; Wu, Ximei M

    2016-03-01

    Endochondral ossification consists of successive steps of chondrocyte differentiation, including mesenchymal condensation, differentiation of chondrocytes, and hypertrophy followed by mineralization and ossification. Loss-of-function studies have revealed that abnormal growth plate cartilage of the Cdc42 mutant contributes to the defects in endochondral bone formation. Here, we have investigated the roles of Cdc42 in osteogenesis and signaling cascades governing Cdc42-mediated chondrogenic differentiation. Though deletion of Cdc42 in limb mesenchymal progenitors led to severe defects in endochondral ossification, either ablation of Cdc42 in limb preosteoblasts or knockdown of Cdc42 in vitro had no obvious effects on bone formation and osteoblast differentiation. However, in Cdc42 mutant limb buds, loss of Cdc42 in mesenchymal progenitors led to marked inactivation of p38 and Smad1/5, and in micromass cultures, Cdc42 lay on the upstream of p38 to activate Smad1/5 in bone morphogenetic protein-2-induced mesenchymal condensation. Finally, Cdc42 also lay on the upstream of protein kinase B to transactivate Sox9 and subsequently induced the expression of chondrocyte differential marker in transforming growth factor-β1-induced chondrogenesis. Taken together, by using biochemical and genetic approaches, we have demonstrated that Cdc42 is involved not in osteogenesis but in chondrogenesis in which the BMP2/Cdc42/Pak/p38/Smad signaling module promotes mesenchymal condensation and the TGF-β/Cdc42/Pak/Akt/Sox9 signaling module facilitates chondrogenic differentiation. Copyright © 2016 by the Genetics Society of America.

  6. El Sistema Universitario Ecuatoriano

    Directory of Open Access Journals (Sweden)

    Lilian Molina

    2016-12-01

    Full Text Available El presente trabajo muestra al sistema universitario ecuatoriano, con el fin de ayudar a entender el inicio de sus reformas en relación con la nueva Constitución y Ley Orgánica de Educación Superior, la importancia de la gratuidad en este nuevo escenario, así como también las nuevas tipologías y orden constitucional de creación de nuevas universidades para promover la investigación científica. Abstract  The present work shows the Ecuadorian university system, in order to help understand the beginning of its reforms in relation to the new Constitution and Organic Law of Higher Education, the importance of gratuitousness in this new scenario, as well as the new typologies And constitutional order of creation of new universities to promote scientific research.

  7. Desafios da pesquisa em comunicação e a infância no noticiário latino-americano

    OpenAIRE

    Rosa, Rosane

    2006-01-01

    Este artigo apresenta o relato da pesquisa intitulada Conocer para íntervenir: infancia, violencia y medias, que envolveu universidades em oito países latino-americanos, para a análise da representação social da infância e da adolescência no jornalismo impresso e televisivo e na ficção. A ênfase neste texto, no entanto, é a representação no jornalismo. Inicialmente, são apresentados alguns desafios da pesquisa em comunicação, e posteriormente estão relatados os principais resultados...

  8. EL PELÍCANO BLANCO AMERICANO (PELECANUS ERYTHRORHYNCHUS EN COLOMBIA, CON COMENTARIOS SOBRE LOS EFECTOS DE LOS HURACANES EN EL CARIBE

    Directory of Open Access Journals (Sweden)

    ESTELA FELIPE A.

    2005-12-01

    Full Text Available Presentamos un registro fotográfico del Pelicano Blanco Americano (Pelecanus erythrorhynchus en un manglar de la isla de San Andrés, en el Caribe colombiano, con lo cual se confirma la presencia de esta especie en Colombia. Adicionalmente discutimos el posible efecto de los huracanes del Caribe en la dispersión de esta especie y los efectos que estas catástrofes naturales pueden tener en la conservación de la avifauna del Archipiélago de San Andrés y Providencia.

  9. EL PELÍCANO BLANCO AMERICANO (PELECANUS ERYTHRORHYNCHUS) EN COLOMBIA, CON COMENTARIOS SOBRE LOS EFECTOS DE LOS HURACANES EN EL CARIBE

    OpenAIRE

    ESTELA FELIPE A.; SILVA JOHN DOUGLAS; CASTILLO LUIS FERNANDO

    2005-01-01

    Presentamos un registro fotográfico del Pelicano Blanco Americano (Pelecanus erythrorhynchus) en un manglar de la isla de San Andrés, en el Caribe colombiano, con lo cual se confirma la presencia de esta especie en Colombia. Adicionalmente discutimos el posible efecto de los huracanes del Caribe en la dispersión de esta especie y los efectos que estas catástrofes naturales pueden tener en la conservación de la avifauna del Archipiélago de San Andrés y Providencia.

  10. A atuação da Organização dos Estados Americanos (OEA nas crises políticas contemporâneas

    Directory of Open Access Journals (Sweden)

    Pedro Ernesto Fagundes

    2010-05-01

    Full Text Available Este artigo analisa a atuação da Organização dos Estados Americanos (OEA no período pós Guerra Fria, especificamente, nas crises políticas do Haiti (1991, Venezuela (2001 e Honduras (2009. O trabalho apresenta um panorama da atuação dessa organização multilateral a partir da década de 1950 até as mais recentes disputas ocorridas na América Latina.

  11. A construção do pan-americanismo na revista Em Guarda: o olhar americano pela defesa das Américas (1941-1946)

    OpenAIRE

    Silva, Julio Cesar dos Santos [UNESP

    2009-01-01

    Durante o período de publicação da revista Em Guarda (1941-1946) o panamericanismo apresentou-se como tema privilegiado nas relações diplomáticas entre os Estados Unidos e a América Latina. No decorrer de suas páginas, os norteamericanos apareciam como modelo de sociedade – político, cultural e sociedade – enquanto aos latino-americanos cabia o papel de fornecer matérias-primas para a fabricação de produtos voltados à guerra. Editada pela agência comandada por Nelson Rockefeller, Em Guarda co...

  12. Comparação do uso de drogas entre universitários brasileiros, norte-americanos e jovens da população geral brasileira

    Directory of Open Access Journals (Sweden)

    Frederico Eckschmidt

    2013-09-01

    Full Text Available OBJETIVO: Comparar o uso de drogas entre os universitários e a população geral brasileira, assim como com os universitários norte-americanos, identificando possíveis diferenças de uso pela interferência da cultura. MÉTODOS: Para comparar o uso de drogas na vida entre as populações de interesse, foram utilizados os dados públicos de levantamentos estatísticos brasileiros realizados com universitários e a população geral, assim como levantamento estatístico norte-americano, por meio da comparação de intervalos de confiança (IC 95%. RESULTADOS: O uso de drogas na vida é mais frequente entre os estudantes norte-americanos, que relatam usar mais tabaco, tranquilizantes, maconha, ecstasy, alucinógenos, cocaína, crack e heroína que os universitários brasileiros. Em contrapartida, os universitários brasileiros relatam usar quase duas vezes mais inalantes do que os universitários norte-americanos. Esse padrão se repete ao se analisarem as diferenças intragênero. A isso se soma que os universitários brasileiros parecem envolver-se com mais frequência no uso de bebidas alcoólicas, maconha, tranquilizantes, inalantes, alucinógenos e anfetamínicos que seus pares da população geral brasileira. CONCLUSÕES: Conhecer as particularidades do uso de drogas entre os universitários é de suma importância para a detecção precoce desse consumo. Investimentos nesse segmento e diferenças de sexo devem ser contempladas nas políticas preventivas, assim como na elaboração de estratégias para a redução e o controle desse consumo, como programas de triagem e intervenção breve, uma vez que possuem a melhor relação custo-benefício.

  13. Estudio poblacional del visón americano ("Mustela vison") en Cataluña: ecología y control de una especie invasora

    OpenAIRE

    Melero Cavero, Yolanda

    2007-01-01

    Desde la introducción del visón americano en Europa, esta especie se ha convertido en un problema ecológico que implica la necesidad de su control. Existen varios métodos de control; sin embargo, actualmente, la opción más práctica y factible de control es el trampeo, combinado con la monitorización de las poblaciones para conocer su estado y dinámica poblacional. De ahí, que el objetivo principal de este estudio es proporcionar estrategias eficaces para el control de la población en Cataluña...

  14. Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F

    DEFF Research Database (Denmark)

    Hateboer, G; Wobst, A; Petersen, B O

    1998-01-01

    functions similar to those of E2F proteins in higher eukaryotes, by regulating the timed expression of genes implicated in cell cycle progression and DNA synthesis. The CDC6 gene is a target for MBF and SBF-regulated transcription. S. cerevisiae Cdc6p induces the formation of the prereplication complex......The E2F transcription factors are essential regulators of cell growth in multicellular organisms, controlling the expression of a number of genes whose products are involved in DNA replication and cell proliferation. In Saccharomyces cerevisiae, the MBF and SBF transcription complexes have...... and is essential for initiation of DNA replication. Interestingly, the Cdc6p homolog in Schizosaccharomyces pombe, Cdc18p, is regulated by DSC1, the S. pombe homolog of MBF. By cloning the promoter for the human homolog of Cdc6p and Cdc18p, we demonstrate here that the cell cycle-regulated transcription...

  15. A hard lesson for Europeans: the ASEAN CDC.

    Science.gov (United States)

    Tibayrenc, Michel

    2005-06-01

    Despite the growing threat of major pandemics, the European Union is planning no more than a meager surveillance agency staffed with 70 people on the 2007 horizon: the new European Centre for Disease Control. I argue that an effective structure should be much larger and include a strong research activity. Asian countries, inspired by the US CDC, are now taking this concept in hand and creating an ASEAN Center For Disease Control, with sophisticated laboratory facilities to be included. This is a tough lesson for us Europeans, and our avarice in this domain could have tragic consequences in the future.

  16. CDC Vital Signs-E-cigarette Ads and Youth

    Centers for Disease Control (CDC) Podcasts

    2016-01-05

    This podcast is based on the January 2016 CDC Vital Signs report. Most electronic cigarettes, or e-cigarettes, contain nicotine, which is highly addictive and may harm brain development. More than 18 million middle and high school students were exposed to e-cigarette ads. Exposure to these ads may be contributing to an increase in e-cigarette use among youth. Learn what can be done to keep our youth safe and healthy.  Created: 1/5/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 1/5/2016.

  17. Large scale chromatographic separations using continuous displacement chromatography (CDC)

    International Nuclear Information System (INIS)

    Taniguchi, V.T.; Doty, A.W.; Byers, C.H.

    1988-01-01

    A process for large scale chromatographic separations using a continuous chromatography technique is described. The process combines the advantages of large scale batch fixed column displacement chromatography with conventional analytical or elution continuous annular chromatography (CAC) to enable large scale displacement chromatography to be performed on a continuous basis (CDC). Such large scale, continuous displacement chromatography separations have not been reported in the literature. The process is demonstrated with the ion exchange separation of a binary lanthanide (Nd/Pr) mixture. The process is, however, applicable to any displacement chromatography separation that can be performed using conventional batch, fixed column chromatography

  18. CDC Vital Signs-Daily Pill Can Prevent HIV

    Centers for Disease Control (CDC) Podcasts

    2015-11-24

    This podcast is based on the November 24, 2015 CDC Vital Signs report. Preexposure prophylaxis, or PrEP, is a daily medicine that can be used to prevent getting HIV. PrEP is for people who don’t have HIV but who are at very high risk for getting it from sex or injection drug use. Unfortunately, many people who can benefit from PrEP aren’t taking it.  Created: 11/24/2015 by National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP).   Date Released: 11/24/2015.

  19. Implementation experiences of NASTRAN on CDC CYBER 74 SCOPE 3.4 operating system

    Science.gov (United States)

    Go, J. C.; Hill, R. G.

    1973-01-01

    The implementation of the NASTRAN system on the CDC CYBER 74 SCOPE 3.4 Operating System is described. The flexibility of the NASTRAN system made it possible to accomplish the change with no major problems. Various sizes of benchmark and test problems, ranging from two hours to less than one minute CP time were run on the CDC CYBER SCOPE 3.3, Univac EXEC-8, and CDC CYBER SCOPE 3.4. The NASTRAN installation deck is provided.

  20. Incunables bogotanos y americanos

    Directory of Open Access Journals (Sweden)

    Manuel José Forero

    1962-04-01

    Full Text Available El interés que despiertan en los círculos cultos las primeras manifestaciones del arte de la imprenta se explica y justifica por varios conceptos. En la duración de muchos siglos el pensamiento humano se enfrentó a dificultades numerosas para lograr su conservación o su perdurabilidad.

  1. El primer virreinato americano

    Directory of Open Access Journals (Sweden)

    Cassá, Roberto

    2006-12-01

    Full Text Available This article explores the government of viceroy Christopher Columbus in the American territories. We return to the first Spanish settlement in Santo Domingo and the contradictions inherent to this expansionist proyect. The contradictions were part of the logic of the absolutist state and Columbus’ reaction against the controls imposed by the monarchs. Secondly, we look into the dificulties that the Admiral encountered to develop a mercantilist model. In this context, we examine the rationale behind the first government of the Indies and the features that defined the new West Indian society.

    El artículo trata sobre el gobierno de Cristóbal Colón en tierras americanas. Retomamos el tema del primer emplazamiento español en Santo Domingo y las contradicciones que tuvo aquel proyecto debido a la lógica del estado absolutista, a la ambición desmedida del descubridor y a su reacción ante los controles que desde un principio impusieron los monarcas. En un segundo momento analizamos las dificultades que encontró el Almirante para desarrollar un modelo mercantilista acorde a sus ideas y a los acuerdos a que llegó con la Corona. En ese contexto analizamos la lógica del primer gobierno colombinista en las Indias y los rasgos que definieron la nueva sociedad antillana.

  2. Integracija ERP in MES sistema

    OpenAIRE

    Oman, Simon

    2016-01-01

    Doktorska disertacija obravnava integracijo poslovnega informacijskega sistema (ERP) in sistema za upravljanje proizvodnje (MES). Podatki v ERP nastajajo kot posledica poslovnih dogodkov in so relativno majhni po obsegu in redki v primerjavi z obsegom in frekvenco podatkov v MES. Zadnje je treba zajemati in obdelovati v realnem času, medtem ko obdelava poslovnih podatkov prenese večurne zakasnitve. Raziskava je osredotočena na konkreten informacijski sistem v poslovnem okolju avtomobilske...

  3. Sistemas distribuidos: control de concurrencia

    OpenAIRE

    Cenci, Karina M.; Ardenghi, Jorge Raúl

    2001-01-01

    Los sistemas distribuidos son requeridos para incrementar la potencialidad de los servicios, aumentando la performance (velocidad), la tolerancia y la seguridad. Estos funcionan sobre grandes redes físicas, cuyo comportamiento puede no ser predecible. Este tipo de sistemas está formado por procesos y recursos / servicios que son compartidos por los mismos. Para el correcto funcionamiento de los procesos es necesario contar con mecanismos que sincronicen los procesos cooperantes, o que cont...

  4. Moderate variations in CDC25B protein levels modulate the response to DNA damaging agents

    International Nuclear Information System (INIS)

    Aressy, B.; Bugler, B.; Valette, A.; Ducommun, B.; Biard, D.

    2008-01-01

    CDC25B, one of the three members of the CDC25 dual-specificity phosphatase family, plays a critical role in the control of the cell cycle and in the checkpoint response to DNA damage. CDC25B is responsible for the initial dephosphorylation and activation of the cyclin-dependent kinases, thus initiating the train of events leading to entry into mitosis. The critical role played by CDC25B is illustrated by the fact that it is specifically required for checkpoint recovery and that unscheduled accumulation of CDC25B is responsible for illegitimate entry into mitosis. Here, we report that in p53 colon carcinoma cells, a moderate increase in the CDC25B level is sufficient to impair the DNA damage checkpoint, to increase spontaneous mutagenesis, and to sensitize cells to ionising radiation and genotoxic agents. Using a tumour cell spheroid assay as an alternative to animal studies, we demonstrate that the level of CDC25B expression modulates growth inhibition and apoptotic death. Since CDC25B overexpression has been observed in a significant number of human cancers, including colon carcinoma, and is often associated with high grade tumours and poor prognosis, our work suggests that the expression level of CDC25B might be a potential key parameter of the cellular response to cancer therapy. (authors)

  5. Moderate variations in CDC25B protein levels modulate the response to DNA damaging agents

    Energy Technology Data Exchange (ETDEWEB)

    Aressy, B.; Bugler, B.; Valette, A.; Ducommun, B. [Univ Toulouse, LBCMCP, CNRS, IFR109, Inst Explorat Fonct Genomes, Toulouse (France); CHU Purpan, Toulouse (France); Biard, D. [CEA, DSV, IRCM/SRO/LGR, F-92265 Fontenay aux Roses Cedex (France)

    2008-07-01

    CDC25B, one of the three members of the CDC25 dual-specificity phosphatase family, plays a critical role in the control of the cell cycle and in the checkpoint response to DNA damage. CDC25B is responsible for the initial dephosphorylation and activation of the cyclin-dependent kinases, thus initiating the train of events leading to entry into mitosis. The critical role played by CDC25B is illustrated by the fact that it is specifically required for checkpoint recovery and that unscheduled accumulation of CDC25B is responsible for illegitimate entry into mitosis. Here, we report that in p53 colon carcinoma cells, a moderate increase in the CDC25B level is sufficient to impair the DNA damage checkpoint, to increase spontaneous mutagenesis, and to sensitize cells to ionising radiation and genotoxic agents. Using a tumour cell spheroid assay as an alternative to animal studies, we demonstrate that the level of CDC25B expression modulates growth inhibition and apoptotic death. Since CDC25B overexpression has been observed in a significant number of human cancers, including colon carcinoma, and is often associated with high grade tumours and poor prognosis, our work suggests that the expression level of CDC25B might be a potential key parameter of the cellular response to cancer therapy. (authors)

  6. Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13.

    Science.gov (United States)

    Gao, Hua; Toro, Tasha B; Paschini, Margherita; Braunstein-Ballew, Bari; Cervantes, Rachel B; Lundblad, Victoria

    2010-12-01

    In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action. A current model proposes that Tel1 binding to telomeres marks them for elongation, as the result of phosphorylation of a proposed S/TQ cluster in the telomerase recruitment domain of Cdc13. However, three observations presented here argue against one key aspect of this model. First, the pattern of Cdc13 phosphatase-sensitive isoforms is not altered by loss of Tel1 function or by mutations introduced into two conserved serines (S249 and S255) in the Cdc13 recruitment domain. Second, an interaction between Cdc13 and Est1, as monitored by a two-hybrid assay, is dependent on S255 but Tel1-independent. Finally, a derivative of Cdc13, cdc13-(S/TQ)11→(S/TA)11, in which every potential consensus phosphorylation site for Tel1 has been eliminated, confers nearly wild-type telomere length. These results are inconsistent with a model in which the Cdc13-Est1 interaction is regulated by Tel1-mediated phosphorylation of the Cdc13 telomerase recruitment domain. We propose an alternative model for the role of Tel1 in telomere homeostasis, which is based on the assumption that Tel1 performs the same molecular task at double-strand breaks (DSBs) and chromosome termini.

  7. The cell polarity determinant CDC42 controls division symmetry to block leukemia cell differentiation.

    Science.gov (United States)

    Mizukawa, Benjamin; O'Brien, Eric; Moreira, Daniel C; Wunderlich, Mark; Hochstetler, Cindy L; Duan, Xin; Liu, Wei; Orr, Emily; Grimes, H Leighton; Mulloy, James C; Zheng, Yi

    2017-09-14

    As a central regulator of cell polarity, the activity of CDC42 GTPase is tightly controlled in maintaining normal hematopoietic stem and progenitor cell (HSC/P) functions. We found that transformation of HSC/P to acute myeloid leukemia (AML) is associated with increased CDC42 expression and activity in leukemia cells. In a mouse model of AML, the loss of Cdc42 abrogates MLL-AF9 -induced AML development. Furthermore, genetic ablation of CDC42 in both murine and human MLL-AF9 (MA9) cells decreased survival and induced differentiation of the clonogenic leukemia-initiating cells. We show that MLL-AF9 leukemia cells maintain cell polarity in the context of elevated Cdc42-guanosine triphosphate activity, similar to nonmalignant, young HSC/Ps. The loss of Cdc42 resulted in a shift to depolarized AML cells that is associated with a decrease in the frequency of symmetric and asymmetric cell divisions producing daughter cells capable of self-renewal. Importantly, we demonstrate that inducible CDC42 suppression in primary human AML cells blocks leukemia progression in a xenograft model. Thus, CDC42 loss suppresses AML cell polarity and division asymmetry, and CDC42 constitutes a useful target to alter leukemia-initiating cell fate for differentiation therapy. © 2017 by The American Society of Hematology.

  8. CDC73 intragenic deletion in familial primary hyperparathyroidism associated with parathyroid carcinoma.

    Science.gov (United States)

    Korpi-Hyövälti, Eeva; Cranston, Treena; Ryhänen, Eeva; Arola, Johanna; Aittomäki, Kristiina; Sane, Timo; Thakker, Rajesh V; Schalin-Jäntti, Camilla

    2014-09-01

    CDC73 mutations frequently underlie the hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism (FIHP), and parathyroid carcinoma. It has also been suggested that CDC73 deletion analysis should be performed in those patients without CDC73 mutations. To investigate for CDC73 deletion in a family with FIHP previously reported not to have CDC73 mutations. Eleven members (six affected with primary hyperparathyroidism and five unaffected) were ascertained from the family, and multiplex ligation-dependent probe amplification was performed to detect CDC73 deletion using leukocyte DNA. A previously unreported deletion of CDC73 involving exons 1-10 was detected in five affected members and two unaffected members who were 26 and 39 years of age. Two affected members had parathyroid carcinomas at the ages of 18 and 32 years, and they had Ki-67 proliferation indices of 5 and 14.5% and did not express parafibromin, encoded by CDC73. Primary hyperparathyroidism in the other affected members was due to adenomas and atypical adenomas, and none had jaw tumors. Two affected members had thoracic aortic aneurysms, which in one member occurred with parathyroid carcinoma and renal cysts. A previously unreported intragenic deletion of exons 1 to 10 of CDC73 was detected in a three-generation family with FIHP, due to adenomas, atypical adenomas, and parathyroid carcinomas. In addition, two affected males had thoracic aortic aneurysms, which may represent another associated clinical feature of this disorder.

  9. El siglo perdido del Derecho Administrativo americano: más allá de un desfase histórico

    Directory of Open Access Journals (Sweden)

    Manuela Canal Silva

    2013-12-01

    Full Text Available Creando la Constitución Administrativa. Los Cien Años Perdidos del Derecho Administrativo americano, elegido por la American Bar Association –ABA– como el mejor trabajo de Derecho Administrativo publicado en el 2012, es considerado una de las más polémicas y exhaustivas exposiciones alrededor de la evolución del gobierno estadinense2. Acorde con las palabras de Robert L. Rabin (s.f., la obra es una contribución sustancial al entendimiento del gobierno administrativo en los Estados Unidos en sus orígenes y actualidad. Este artículo presenta las ideas más importantes del trabajo de Mashaw analizando la evolución de la Administración desde los primeros días de la República americana. Con este fin, explora la configuración del gobierno americano antes y después de la guerra civil. En conclusión, el artículo desarrolla la exposición de Mashaw y explora las implicaciones de su obra en las disciplinas histórica y jurídica americanas.

  10. EUROPA Y SU PERCEPCIÓN DEL NUEVO MUNDO A TRAVÉS DE LAS ESPECIES COMESTIBLES Y LOS ESPACIOS AMERICANOS EN EL SIGLO XVI

    Directory of Open Access Journals (Sweden)

    OLAYA SANFUENTES ECHEVERRÍA

    2006-12-01

    Full Text Available El encuentro europeo con las nuevas especies y espacios americanos en el siglo XVI, complejiza ese universo de percepciones de superioridad occidental frente a mundos que se consideraban más exóticos y menos civilizados. Las descripciones europeas de la naturaleza americana, las primeras imágenes de sus productos alimenticios y especies autóctonas pintadas con los colores del paraíso, el exotismo y la belleza, la fertilidad y la abundancia, ayudarán a conformar una imagen positiva del continente americano. A pesar del original desprecio o indiferencia frente a ciertos alimentos indígenas, con el tiempo estas percepciones cambiarán al convertirse muchos de ellos en salvavidas de las hambrunas mundiales e ingredientes fundamentales de las gastronomías nacionalesThe European encounter with the new American space and it species during the sixteenth century, allows us to look at the European percepction of superiority towards exotic worlds in a more complex way. European description of American nature, the first visual images of the local species described with the colors of paradise, exotism and beauty, fertility and abundance, help in constructing a positive image of America. Despite the original disdain or indifference towards the indigenous food, these percepctions will change over time when many of these products become a solution to world wide hunger and important ingredients for national gastronomies

  11. Los indios americanos en las escenas líricas europeas: de los hermanos Purcell (1695 a Carl Heinrich Graun (1755

    Directory of Open Access Journals (Sweden)

    Núñez Ronchi, Ana

    2014-08-01

    Full Text Available In contrast to a supposedly critically-oriented Golden Age comedia, the first American- themed operas (the Purcells, Vivaldi, Rameau, Graun have been interpreted as uncritical expressions of the so-called Black Legend. Nevertheless, this judgment ought to be tempered: on the one hand, the Spanish comedia is not as censorious as has been asserted; on the other hand, although these operas do praise American Indians, they do not systematically belittle the Spaniards. Finally, the notion itself of “Black Legend” should be questioned.Se ha querido interpretar las primeras óperas de tema americano (Purcell, Vivaldi, Rameau y Graun como una expresión dramático-musical de la llamada Leyenda Negra, contrapuesta a una producción teatral áurea española que, inversamente, sí habría dejado espacio a la dimensión histórico-crítica. Ello nos parece injusto e inexacto: en primer lugar, la comedia áurea no escenifica la dimensión crítica que se ha pretendido atribuirle; en segundo lugar, el teatro lírico europeo, si bien exalta al indio americano, no devalúa sistemáticamente a los conquistadores españoles; finalmente, la noción misma de «Leyenda Negra» debe ser problematizada.

  12. Instrumentos marginais de percussão na música contemporânea: uma expansão técnico-interpretativa de instrumentos latino-americanos

    OpenAIRE

    Santos, Bruno Soares

    2015-01-01

    O presente trabalho propõe-se a investigar estruturas interpretativas em obras para instrumentos latino-americanos explorando os conceitos de ‘instrumento marginal’ e ‘literatura menor’. Esta investigação desenvolveu-se através de três estudos de caso. O primeiro estudo de caso concentra-se na obra Temazcal de Javier Alvarez para maracas e tape. O segundo foca-se no repertório para tambores de mão latino-americanos. O terceiro sugere uma estrutura para uma improvisação para ...

  13. Draft Genome Assemblies of Enterobacter aerogenes CDC 6003-71, Enterobacter cloacae CDC 442-68, and Pantoea agglomerans UA 0804-01.

    Science.gov (United States)

    Minogue, T D; Daligault, H E; Davenport, K W; Bishop-Lilly, K A; Bruce, D C; Chain, P S; Coyne, S R; Chertkov, O; Freitas, T; Frey, K G; Jaissle, J; Koroleva, G I; Ladner, J T; Palacios, G F; Redden, C L; Xu, Y; Johnson, S L

    2014-10-23

    The Enterobacteriaceae are environmental and enteric microbes. We sequenced the genomes of two Enterobacter reference strains, E. aerogenes CDC 6003-71 and E. cloacae CDC 442-68, as well as one near neighbor used as an exclusionary reference for diagnostics, Pantoea agglomerans CDC UA0804-01. The genome sizes range from 4.72 to 5.55 Mbp and have G+C contents from 54.6 to 55.1%. Copyright © 2014 Minogue et al.

  14. Gene targeting implicates Cdc42 GTPase in GPVI and non-GPVI mediated platelet filopodia formation, secretion and aggregation.

    Directory of Open Access Journals (Sweden)

    Huzoor Akbar

    Full Text Available Cdc42 and Rac1, members of the Rho family of small GTPases, play critical roles in actin cytoskeleton regulation. We have shown previously that Rac1 is involved in regulation of platelet secretion and aggregation. However, the role of Cdc42 in platelet activation remains controversial. This study was undertaken to better understand the role of Cdc42 in platelet activation.We utilized the Mx-cre;Cdc42(lox/lox inducible mice with transient Cdc42 deletion to investigate the involvement of Cdc42 in platelet function. The Cdc42-deficient mice exhibited a significantly reduced platelet count than the matching Cdc42(+/+ mice. Platelets isolated from Cdc42(-/-, as compared to Cdc42(+/+, mice exhibited (a diminished phosphorylation of PAK1/2, an effector molecule of Cdc42, (b inhibition of filopodia formation on immobilized CRP or fibrinogen, (c inhibition of CRP- or thrombin-induced secretion of ATP and release of P-selectin, (d inhibition of CRP, collagen or thrombin induced platelet aggregation, and (e minimal phosphorylation of Akt upon stimulation with CRP or thrombin. The bleeding times were significantly prolonged in Cdc42(-/- mice compared with Cdc42(+/+ mice.Our data demonstrate that Cdc42 is required for platelet filopodia formation, secretion and aggregation and therefore plays a critical role in platelet mediated hemostasis and thrombosis.

  15. Del sistema al actor

    Directory of Open Access Journals (Sweden)

    Alain Touraine

    2003-01-01

    Full Text Available Si consideramos que las conductas sociales están determinadas por el lugar que los individuos, los grupos o las categorías sociales ocupan en la organización social, entonces será necesario aceptar la idea de que aquello que se denomina la Sociedad posee sus propios intereses y refuerza su integración y funcionamiento. Esta idea ha encontrado dos expresiones concretas: o bien se piensa que el bien y el mal son definidos por aquello que es funcional o disfuncional para la sociedad, es decir que se define por su integración, por las conductas de sus miembros, o bien se prefiere afirmar que la sociedad no es un sistema sino un conjunto de medios dirigidos por una clase o una elite dirigente. Se trata de dos versiones diferentes de lo que se puede denominar sociologismo, es decir, considerar que el interés de la totalidad se impone a sus partes, como el cuerpo se impone a los órganos en la concepción biológica tradicional. Se puede distinguir también la sociología funcionalista sistémica de la sociología funcionalista crítica. En ambos casos se trata de dos de las orientaciones mas importantes del pensamiento sociológico desde sus orígenes.

  16. CDC Signos Vitales: Enfermedad del legionario (CDC Vital Signs–Legionnaires’ Disease)

    Centers for Disease Control (CDC) Podcasts

    2016-06-07

    Este podcast se basa en la edición de junio del 2016 del informe Signos Vitales de los CDC. Las personas pueden contraer la enfermedad del legionario, un tipo grave de infección pulmonar, al inhalar pequeñas gotitas de agua contaminada con bacterias Legionella. Obtenga más información sobre lo que se puede hacer para ayudar a prevenir brotes de enfermedad del legionario y mantener seguras a las personas.  Created: 6/7/2016 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 6/7/2016.

  17. Um exemplo de inversão do domínio ideológico norte-americano no Brasil

    Directory of Open Access Journals (Sweden)

    Carla Damião

    2014-07-01

    Full Text Available Propomos discutir a relação de domínio ideológico-cultural norte-americano sobre o Brasil, tendo em vista exemplos documentados. Trata-se da chamada “política de boa vizinhança” [Good Neighbor Policy], com início em 1933 até o final da Segunda Guerra, notadamente durante o governo Getúlio Vargas no Brasil e o governo Franklin Roosevelt nos Estados Unidos. A necessidade de propaganda política passava pela “sedução” cultural da potência estadunidense sobre seus vizinhos latino-americanos. Neste sentido, o momento político de tentativa de “adoção” ideológica do Brasil pelos Estados Unidos foi marcado pela “encomenda” de produtos culturais como a personagem do malandro Zé Carioca de Disney. O exemplo mais ambíguo que marca essa ambição de domínio ideológico é o filme encomendado a Orson Welles e jamais finalizado, intitulado: It’s All True [É tudo verdade]. O filme não concluído por seu famoso diretor, filmado em 1942, só se tornou conhecido em 1993 quando outros diretores americanos realizaram um documentário remontando partes do filme. O material presente nesse documentário foi também recuperado pela pesquisa realizada pelo cineasta brasileiro Rogério Sganzerla, da qual surgiu uma tetralogia composta pelos filmes: Nem tudo é verdade (1986, A liguagem de orson Welles (1990, Tudo é Brasil (1997 e O signo do caos (2003. O projeto de domínio cultural encomendado a Orson Welles terminou em fracasso, visto que este desviou sua atenção da glamourosa cidade do Rio de Janeiro e do Carnaval, para filmar a saga de jangadeiros que lutavam por direitos trabalhistas. A conotação política do filme determina seu fracasso no domínio ideológico de propaganda política do governo americano. Sganzerla elegeu o episódio para pensar e criticar o domínio ideológico norte-americano sempre presente na cultura brasileira, de uma maneira bastante ambígua. A ambiguidade permeia a atitude dos dois cineastas: o

  18. Rho GTPase protein Cdc42 is critical for postnatal cartilage development

    Energy Technology Data Exchange (ETDEWEB)

    Nagahama, Ryo [Department of Biochemistry, School of Dentistry, Showa University, Tokyo (Japan); Department of Orthodontics, School of Dentistry, Showa University, Tokyo (Japan); Yamada, Atsushi, E-mail: yamadaa@dent.showa-u.ac.jp [Department of Biochemistry, School of Dentistry, Showa University, Tokyo (Japan); Tanaka, Junichi [Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, Tokyo (Japan); Aizawa, Ryo [Department of Periodontology, School of Dentistry, Showa University, Tokyo (Japan); Suzuki, Dai [Department of Biochemistry, School of Dentistry, Showa University, Tokyo (Japan); Kassai, Hidetoshi [Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Tokyo (Japan); Yamamoto, Matsuo [Department of Periodontology, School of Dentistry, Showa University, Tokyo (Japan); Mishima, Kenji [Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, Tokyo (Japan); Aiba, Atsu [Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Tokyo (Japan); Maki, Koutaro [Department of Orthodontics, School of Dentistry, Showa University, Tokyo (Japan); Kamijo, Ryutaro [Department of Biochemistry, School of Dentistry, Showa University, Tokyo (Japan)

    2016-02-19

    Cdc42, a small Rho GTPase family member, has been shown to regulate multiple cellular functions in vitro, including actin cytoskeletal reorganization, cell migration, proliferation, and gene expression. However, its tissue-specific roles in vivo remain largely unknown, especially in postnatal cartilage development, as cartilage-specific Cdc42 inactivated mice die within a few days after birth. In this study, we investigated the physiological functions of Cdc42 during cartilage development after birth using tamoxifen-induced cartilage-specific inactivated Cdc42 conditional knockout (Cdc42 {sup fl/fl}; Col2-CreERT) mice, which were generated by crossing Cdc42 flox mice (Cdc42 {sup fl/fl}) with tamoxifen-induced type II collagen (Col2) Cre transgenic mice using a Cre/loxP system. The gross morphology of the Cdc42 cKO mice was shorter limbs and body, as well as reduced body weight as compared with the controls. In addition, severe defects were found in growth plate chondrocytes of the long bones, characterized by a shorter proliferating zone (PZ), wider hypertrophic zone (HZ), and loss of columnar organization of proliferating chondrocytes, resulting in delayed endochondral bone formation associated with abnormal bone growth. Our findings demonstrate the importance of Cdc42 for cartilage development during both embryonic and postnatal stages. - Highlights: • Tamoxifen-induced cartilage specific inactivated Cdc42 mutant mice were generated. • Cdc42 mutant mice were shorter limbs and body. • Severe defects were found in growth plate chondrocytes.

  19. Guanine nucleotide induced conformational change of Cdc42 revealed by hydrogen/deuterium exchange mass spectrometry.

    Science.gov (United States)

    Yang, Sheng-Wei; Ting, Hsiu-Chi; Lo, Yi-Ting; Wu, Ting-Yuan; Huang, Hung-Wei; Yang, Chia-Jung; Chan, Jui-Fen Riva; Chuang, Min-Chieh; Hsu, Yuan-Hao Howard

    2016-01-01

    Cdc42 regulates pathways related to cell division. Dysregulation of Cdc42 can lead to cancer, cardiovascular diseases and neurodegenerative diseases. GTP induced activation mechanism plays an important role in the activity and biological functions of Cdc42. P-loop, Switch I and Switch II are critical regions modulating the enzymatic activity of Cdc42. We applied amide hydrogen/deuterium exchange coupled with liquid chromatography mass spectrometry (HDXMS) to investigate the dynamic changes of apo-Cdc42 after GDP, GTP and GMP-PCP binding. The natural substrate GTP induced significant decreases of deuteration in P-loop and Switch II, moderate changes of deuteration in Switch I and significant changes of deuteration in the α7 helix, a region far away from the active site. GTP binding induced similar effects on H/D exchange to its non-hydrolysable analog, GMP-PCP. HDXMS results indicate that GTP binding blocked the solvent accessibility in the active site leading to the decrease of H/D exchange rate surrounding the active site, and further triggered a conformational change resulting in the drastic decrease of H/D exchange rate at the remote α7 helix. Comparing the deuteration levels in three activation states of apo-Cdc42, Cdc42-GDP and Cdc42-GMP-PCP, the apo-Cdc42 has the most flexible structure, which can be stabilized by guanine nucleotide binding. The rates of H/D exchange of Cdc42-GDP are between the GMP-PCP-bound and the apo form, but more closely to the GMP-PCP-bound form. Our results show that the activation of Cdc42 is a process of conformational changes involved with P-loop, Switch II and α7 helix for structural stabilization. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. Rho GTPase protein Cdc42 is critical for postnatal cartilage development

    International Nuclear Information System (INIS)

    Nagahama, Ryo; Yamada, Atsushi; Tanaka, Junichi; Aizawa, Ryo; Suzuki, Dai; Kassai, Hidetoshi; Yamamoto, Matsuo; Mishima, Kenji; Aiba, Atsu; Maki, Koutaro; Kamijo, Ryutaro

    2016-01-01

    Cdc42, a small Rho GTPase family member, has been shown to regulate multiple cellular functions in vitro, including actin cytoskeletal reorganization, cell migration, proliferation, and gene expression. However, its tissue-specific roles in vivo remain largely unknown, especially in postnatal cartilage development, as cartilage-specific Cdc42 inactivated mice die within a few days after birth. In this study, we investigated the physiological functions of Cdc42 during cartilage development after birth using tamoxifen-induced cartilage-specific inactivated Cdc42 conditional knockout (Cdc42  fl/fl ; Col2-CreERT) mice, which were generated by crossing Cdc42 flox mice (Cdc42  fl/fl ) with tamoxifen-induced type II collagen (Col2) Cre transgenic mice using a Cre/loxP system. The gross morphology of the Cdc42 cKO mice was shorter limbs and body, as well as reduced body weight as compared with the controls. In addition, severe defects were found in growth plate chondrocytes of the long bones, characterized by a shorter proliferating zone (PZ), wider hypertrophic zone (HZ), and loss of columnar organization of proliferating chondrocytes, resulting in delayed endochondral bone formation associated with abnormal bone growth. Our findings demonstrate the importance of Cdc42 for cartilage development during both embryonic and postnatal stages. - Highlights: • Tamoxifen-induced cartilage specific inactivated Cdc42 mutant mice were generated. • Cdc42 mutant mice were shorter limbs and body. • Severe defects were found in growth plate chondrocytes.

  1. Blastomicose do sistema nervoso

    Directory of Open Access Journals (Sweden)

    Horácio M. Canelas

    1951-09-01

    Full Text Available Os autores chamam a atenção sôbre os erros de diagnóstico nos casos de neuroblastomicose, seja nas formas meningoencefálicas, seja nas tumorais. São focalizadas a paracoccidioidose e a criptococose. Depois de caracterizarem clìnicamente a paracoccidioidose, acentuam a raridade das formas nervosas (1,2% dos casos dessa micose autopsiados no Departamento de Anatomia Patológica da Faculdade de Medicina da Universidade de São Paulo. Pela revisão da literatura foi verificado haver apenas 12 casos registrados. 0 caso de Casiello e Klass é o único em que o diagnóstico foi feito em vida; os demais, ou constituem achado de necropsia, ou foram inesperadamente revelados pelo exame histopatológico realizado após intervenções cirúrgicas. Os autores registram 2 casos de paracoccidioidose do sistema nervoso. No primeiro, tratava-se de meningomielorra-diculite crônica, sendo o parasito identificado no escarro; o segundo era portador de síndrome de compressão medular, cuja patogenia é discutida pelos autores, tendo sido a etiologia paracoccidióidica comprovada pelo exame da polpa e biópsia ganglionares. Considerações clínicas sôbre a criptococose precedem o relato de um caso de meningoencefalomielite subaguda, no qual fôra feito em vida o diagnóstico de paracoccidioidose, pelo exame micológico do liqüido cefalorraqueano; êste paciente, embora medicado com doses maciças de sulfa e tiossemicarbarsona, veio a falecer, tendo o exame anátomo-patológico revelado tratar-se, na realidade, de lesões nervosas produzidas pelo Crypíococcus neo-formans. Nos três casos era incisivo o caráter neurocirúrgico da sintomatologia. Entretanto, ante o diagnóstico de paracoccidioidose, foi instituído tratamento pelas sulfas, vacina específica e tiossemicarbarsona. Os resultados foram excelentes no caso 2, em que a terapêutica pôde ser instituída mais precocemente; melhoras também foram obtidas no caso 1. No caso 3, foram verificadas

  2. The human homolog of S. cerevisiae CDC27, CDC27 Hs, is encoded by a highly conserved intronless gene present in multiple copies in the human genome

    Energy Technology Data Exchange (ETDEWEB)

    Devor, E.J.; Dill-Devor, R.M. [Univ. of Iowa College of Medicine, Iowa City (United States)

    1994-09-01

    We have obtained a number of unique sequences via PCR amplification of human genomic DNA using degenerate primers under low stringency (42{degrees}C). One of these, an 853 bp product, has been identified as a partial genomic sequence of the human homolog of the S. cerevisiae CDC27 gene, CDC27Hs (GenBank No. U00001). This gene, reported by Turgendreich et al. is also designated EST00556 from Adams et al. We have undertaken a more detailed examination of our sequence, MCP34N, and have found that: 1. the genomic sequence is nearly identical to CDC27Hs over its entire 853 bp length; 2. an MCP34N-specific PCR assay of several non-human primate species reveals amplification products in chimpanzee and gorilla genomes having greater than 90% sequence identity with CDC27Hs; and 3. an MCP34N-specific PCR assay of the BIOS hybrid cell line panel gives a discordancy pattern suggesting multiple loci. Based upon these data, we present the following initial characterization: 1. the complete MCP34N sequence identity with CDC27Hs indicates that the latter is encoded by an intronless gene; 2. CDC27Hs is highly conserved among higher primates; and 3. CDC27Hs is present in multiple copies in the human genome. These characteristics, taken together with those initially reported for CDC27Hs, suggest that this is an old gene that carries out an important but, as yet, unknown function in the human brain.

  3. Synthesis and Molecular modeling of some new chalcones derived from coumarine as CDC25 phosphatases inhibitors

    Directory of Open Access Journals (Sweden)

    Delel Dridi

    2016-01-01

    Full Text Available New chalcones derived from coumarines were synthesized and tested as CDC25 phosphatase inhibitors. Molecular modeling of these new compounds was also presented in aim to study the mode of compounds orientation within CDC25 A and B. The reversibility of compounds 3, 4 and 5 was confirmed by application of MALDI–TOFMS technique.

  4. Distinct roles of Cdc42 in thymopoiesis and effector and memory T cell differentiation.

    Directory of Open Access Journals (Sweden)

    Fukun Guo

    Full Text Available Cdc42 of the Rho GTPase family has been implicated in cell actin organization, proliferation, survival, and migration but its physiological role is likely cell-type specific. By a T cell-specific deletion of Cdc42 in mouse, we have recently shown that Cdc42 maintains naïve T cell homeostasis through promoting cell survival and suppressing T cell activation. Here we have further investigated the involvement of Cdc42 in multiple stages of T cell differentiation. We found that in Cdc42(-/- thymus, positive selection of CD4(+CD8(+ double-positive thymocytes was defective, CD4(+ and CD8(+ single-positive thymocytes were impaired in migration and showed an increase in cell apoptosis triggered by anti-CD3/-CD28 antibodies, and thymocytes were hyporesponsive to anti-CD3/-CD28-induced cell proliferation and hyperresponsive to anti-CD3/-CD28-stimulated MAP kinase activation. At the periphery, Cdc42-deficient naive T cells displayed an impaired actin polymerization and TCR clustering during the formation of mature immunological synapse, and showed an enhanced differentiation to Th1 and CD8(+ effector and memory cells in vitro and in vivo. Finally, Cdc42(-/- mice exhibited exacerbated liver damage in an induced autoimmune disease model. Collectively, these data establish that Cdc42 is critically involved in thymopoiesis and plays a restrictive role in effector and memory T cell differentiation and autoimmunity.

  5. The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface

    DEFF Research Database (Denmark)

    Cappello, Silvia; Attardo, Alessio; Wu, Xunwei

    2006-01-01

    the fundamental difference between these progenitors. Here we show that the conditional deletion of the small Rho-GTPase cdc42 at different stages of neurogenesis in mouse telencephalon results in an immediate increase in basal mitoses. Whereas cdc42-deficient progenitors have normal cell cycle length...

  6. Podocyte-specific loss of cdc42 leads to congenital nephropathy

    DEFF Research Database (Denmark)

    Scott, Rizaldy P; Hawley, Steve P; Ruston, Julie

    2012-01-01

    in the absence of Cdc42, indicating a disruption of the slit diaphragm. Kidneys from Rac1- and RhoA-mutant mice, however, had normal glomerular morphology and intact foot processes. A nephrin clustering assay suggested that Cdc42 deficiency, but not Rac1 or RhoA deficiency, impairs the polymerization of actin...

  7. CDC Vital Signs–Safe Sleep for Babies

    Centers for Disease Control (CDC) Podcasts

    2018-01-09

    This podcast is based on the January 2018 CDC Vital Signs report. Every year, there are about 3,500 sleep-related deaths among U.S. babies. Learn how to create a safe sleep environment for babies.  Created: 1/9/2018 by Centers for Disease Control and Prevention (CDC).   Date Released: 1/9/2018.

  8. Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.

    Directory of Open Access Journals (Sweden)

    Felipe O Bendezú

    2015-04-01

    Full Text Available The small Rho-family GTPase Cdc42 is critical for cell polarization and polarizes spontaneously in absence of upstream spatial cues. Spontaneous polarization is thought to require dynamic Cdc42 recycling through Guanine nucleotide Dissociation Inhibitor (GDI-mediated membrane extraction and vesicle trafficking. Here, we describe a functional fluorescent Cdc42 allele in fission yeast, which demonstrates Cdc42 dynamics and polarization independent of these pathways. Furthermore, an engineered Cdc42 allele targeted to the membrane independently of these recycling pathways by an amphipathic helix is viable and polarizes spontaneously to multiple sites in fission and budding yeasts. We show that Cdc42 is highly mobile at the membrane and accumulates at sites of activity, where it displays slower mobility. By contrast, a near-immobile transmembrane domain-containing Cdc42 allele supports viability and polarized activity, but does not accumulate at sites of activity. We propose that Cdc42 activation, enhanced by positive feedback, leads to its local accumulation by capture of fast-diffusing inactive molecules.

  9. CDC Vital Signs–Opioid Overdoses Treated in Emergency Departments

    Centers for Disease Control (CDC) Podcasts

    2018-03-06

    This podcast is based on the March 2018 CDC Vital Signs report. Opioid overdoses continue to increase in the United States. Learn what can be done to help prevent opioid overdose and death.  Created: 3/6/2018 by Centers for Disease Control and Prevention (CDC).   Date Released: 3/6/2018.

  10. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo

    DEFF Research Database (Denmark)

    Luckashenak, Nancy; Wähe, Anna; Breit, Katharina

    2013-01-01

    and contributions of this cell type. To target the migratory properties of DCs, we generated mice lacking the Rho-family GTPase Cdc42 specifically in DCs. In these animals, the initial seeding of the epidermis with LCs is functional, resulting in slightly reduced Langerhans cell numbers. However, Cdc42-deficient...

  11. Spontaneous cell polarization: Feedback control of Cdc42 GTPase breaks cellular symmetry.

    Science.gov (United States)

    Martin, Sophie G

    2015-11-01

    Spontaneous polarization without spatial cues, or symmetry breaking, is a fundamental problem of spatial organization in biological systems. This question has been extensively studied using yeast models, which revealed the central role of the small GTPase switch Cdc42. Active Cdc42-GTP forms a coherent patch at the cell cortex, thought to result from amplification of a small initial stochastic inhomogeneity through positive feedback mechanisms, which induces cell polarization. Here, I review and discuss the mechanisms of Cdc42 activity self-amplification and dynamic turnover. A robust Cdc42 patch is formed through the combined effects of Cdc42 activity promoting its own activation and active Cdc42-GTP displaying reduced membrane detachment and lateral diffusion compared to inactive Cdc42-GDP. I argue the role of the actin cytoskeleton in symmetry breaking is not primarily to transport Cdc42 to the active site. Finally, negative feedback and competition mechanisms serve to control the number of polarization sites. © 2015 WILEY Periodicals, Inc.

  12. CDC73-Related Disorders: Clinical Manifestations and Case Detection in Primary Hyperparathyroidism

    NARCIS (Netherlands)

    van der Tuin, Karin; Tops, Carli M. J.; Adank, Muriel A.; Cobben, Jan-Maarten; Hamdy, Neveen A. T.; Jongmans, Marjolijn C.; Menko, Fred H.; van Nesselrooij, Bernadette P. M.; Netea-Maier, Romana T.; Oosterwijk, Jan C.; Valk, Gerlof D.; Wolffenbuttel, Bruce H. R.; Hes, Frederik J.; Morreau, Hans

    2017-01-01

    Context: Heterozygous pathogenic germline variants in CDC73 predispose to the development of primary hyperparathyroidism (pHPT) and, less frequently, ossifying fibroma of the jaw and renal and uterine tumors. Clinical information on CDC73-related disorders has so far been limited to small case

  13. Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy.

    Directory of Open Access Journals (Sweden)

    Ronan Broderick

    Full Text Available Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our data show that eGFP-Cdc45 mobility is faster in G1/S transition compared to S phase suggesting that Cdc45 is part of larger protein complex formed in S phase. Furthermore, the size of complexes containing Cdc45 was estimated in asynchronous, G1/S and S phase-synchronized cells using gel filtration chromatography; these findings complemented the in vivo FCS data. Analysis of the mobility of eGFP-Cdc45 and the size of complexes containing Cdc45 and eGFP-Cdc45 after UVC-mediated DNA damage revealed no significant changes in diffusion rates and complex sizes using FCS and gel filtration chromatography analyses. This suggests that after UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage.

  14. NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling

    DEFF Research Database (Denmark)

    Sørensen, Claus Storgaard; Melixetian, Marina; Klein, Ditte Kjaersgaard

    2010-01-01

    The DNA damage induced G(2)/M checkpoint is an important guardian of the genome that prevents cell division when DNA lesions are present. The checkpoint prevents cells from entering mitosis by degrading CDC25A, a key CDK activator. CDC25A proteolysis is controlled by direct phosphorylation events...

  15. Sistemas de tiempo real y sistemas distribuidos de tiempo real

    OpenAIRE

    Pesado, Patricia Mabel; Ramón, Hugo Dionisio; Boracchia, Marcos; Pasini, Ariel C.; De Vito, María Cecilia; Osella Massa, Germán Leandro; Estrebou, César Armando

    2006-01-01

    El proyecto de investigación y desarrollo en Sistemas de Software Distribuido, abarca aspectos que van desde los fundamentos del desarrollo (Técnicas de Ingeniería de Requerimientos, Metodologías de Gestión y Desarrollo de Proyectos, Técnicas de Planificación, Métricas, Normas de Calidad, Web-Systems) hasta la concepción de aplicaciones específicas orientadas a los procesos industriales (Sistemas de Planeamiento de Producción, Control Industrial en tiempo real), procesos de E-government (Voto...

  16. User's manual for the CDC-6600 version of AMPX

    Energy Technology Data Exchange (ETDEWEB)

    Pickard, P. S.; Vandevender, W. H.

    1977-11-01

    A CDC-6600 version of AMPX-I, the Oak Ridge National Laboratory's modular code system for generating coupled multigroup neutron--gamma libraries from ENDF/B data is described. The AMPX modules can generate multigroup neutron cross sections; generate multigroup gamma cross sections; generate gamma yields for gamma-producing neutron interactions; combine neutron cross sections, gamma cross sections, and gamma yields into ''couples sets;'' perform one-dimensional discrete-ordinates-transport or diffusion-theory calculations for neutrons and gammas and, on option, collapse the cross sections to broad-group structure, with the one-dimensional results used as weighting functions; and output multigroup cross sections in convenient formats for other codes. 7 figures, 4 tables.

  17. Raptor is phosphorylated by cdc2 during mitosis.

    Directory of Open Access Journals (Sweden)

    Dana M Gwinn

    2010-02-01

    Full Text Available The appropriate control of mitotic entry and exit is reliant on a series of interlocking signaling events that coordinately drive the biological processes required for accurate cell division. Overlaid onto these signals that promote orchestrated cell division are checkpoints that ensure appropriate mitotic spindle formation, a lack of DNA damage, kinetochore attachment, and that each daughter cell has the appropriate complement of DNA. We recently discovered that AMP-activated protein kinase (AMPK modulates the G2/M phase of cell cycle progression in part through its suppression of mammalian target of rapamycin (mTOR signaling. AMPK directly phosphorylates the critical mTOR binding partner raptor inhibiting mTORC1 (mTOR-raptor rapamycin sensitive mTOR kinase complex 1. As mTOR has been previously tied to mitotic control, we examined further how raptor may contribute to this process.We have discovered that raptor becomes highly phosphorylated in cells in mitosis. Utilizing tandem mass spectrometry, we identified a number of novel phosphorylation sites in raptor, and using phospho-specific antibodies demonstrated that raptor becomes phosphorylated on phospho-serine/threonine-proline sites in mitosis. A combination of site-directed mutagenesis in a tagged raptor cDNA and analysis with a series of new phospho-specific antibodies generated against different sites in raptor revealed that Serine 696 and Threonine 706 represent two key sites in raptor phosphorylated in mitosis. We demonstrate that the mitotic cyclin-dependent kinase cdc2/CDK1 is the kinase responsible for phosphorylating these sites, and its mitotic partner Cyclin B efficiently coimmunoprecipitates with raptor in mitotic cells.This study demonstrates that the key mTOR binding partner raptor is directly phosphorylated during mitosis by cdc2. This reinforces previous studies suggesting that mTOR activity is highly regulated and important for mitotic progression, and points to a direct

  18. Recurrent Hyperparathyroidism Due to a Novel CDC73 Splice Mutation.

    Science.gov (United States)

    Hattangady, Namita Ganesh; Wilson, Tremika Le-Shan; Miller, Barbra Sue; Lerario, Antonio Marcondes; Giordano, Thomas James; Choksi, Palak; Else, Tobias

    2017-08-01

    The recognition of hereditary causes of primary hyperparathyroidism (pHPT) is important because clinical care and surveillance differ significantly between sporadic and hereditary pHPT. In addition, the increasing number of genetic tests poses a challenge to classify mutations as benign or pathogenic. Functional work-up of variants remains a mainstay to provide evidence for pathogenicity. We describe a 52-year-old male patient with recurrent pHPT since age 35 years. Despite several neck surgeries with complete parathyroidectomy, he experienced persistent pHPT, necessitating repeated surgery for a forearm autotransplant, which finally resulted in unmeasurable parathyroid hormone (PTH) levels. Genetic testing revealed a new CDC73 variant (c.238-8G>A [IVS2-8G>A]), initially classified as a variant of uncertain significance. Parathyroid tissue from the initial surgeries showed loss of heterozygosity. Using an RT-PCR approach, we show that the mutation leads to the use of a cryptic splice site in peripheral mononuclear cells. In addition, a minigene approach confirms the use of the cryptic splice site in a heterologous cell system. The novel c.238-8G>A CDC73 variant activates a cryptic splice site, and the functional data provided justify the classification as a likely pathogenic variant. Our results underscore the importance of functional work-up for variant classification in the absence of other available data, such as presence in disease-specific databases, other syndromic clinical findings, or family history. In addition, the presented case exemplifies the importance to consider a hereditary condition in young patients with pHPT, particularly those with multi-gland involvement. © 2017 American Society for Bone and Mineral Research. © 2017 American Society for Bone and Mineral Research.

  19. CDC Kerala 1: Organization of clinical child development services (1987-2013).

    Science.gov (United States)

    Nair, M K C; George, Babu; Nair, G S Harikumaran; Bhaskaran, Deepa; Leena, M L; Russell, Paul Swamidhas Sudhakar

    2014-12-01

    The main objective of establishing the Child Development Centre (CDC), Kerala for piloting comprehensive child adolescent development program in India, has been to understand the conceptualization, design and scaling up of a pro-active positive child development initiative, easily replicable all over India. The process of establishing the Child Development Centre (CDC) Kerala for research, clinical services, training and community extension services over the last 25 y, has been as follows; Step 1: Conceptualization--The life cycle approach to child development; Step 2: Research basis--CDC model early stimulation is effective; Step 3: Development and validation of seven simple developmental screening tools; Step 4: CDC Diagnostic services--Ultrasonology and genetic, and metabolic laboratory; Step 5: Developing seven intervention packages; Step 6: Training--Post graduate diploma in clinical child development; Step 7: CDC Clinic Services--seven major ones; Step 8: CDC Community Services--Child development referral units; Step 9: Community service delivery models--Childhood disability and for adolescent care counselling projects; Step 10: National capacity building--Four child development related courses. CDC Kerala follow-up and clinic services are offered till 18 y of age and premarital counselling till 24 y of age as shown in "CDC Kerala Clinic Services Flow Chart" and 74,291 children have availed CDC clinic services in the last 10 y. CDC Kerala is the first model for comprehensive child adolescent development services using a lifecycle approach in the Government sector and hence declared as the collaborative centre for Rashtriya Bal Swasthya Karyakram (RBSK), in Kerala.

  20. Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1

    DEFF Research Database (Denmark)

    Petersen, B O; Wagener, C; Marinoni, F

    2000-01-01

    CDC6 is conserved during evolution and is essential and limiting for the initiation of eukaryotic DNA replication. Human CDC6 activity is regulated by periodic transcription and CDK-regulated subcellular localization. Here, we show that, in addition to being absent from nonproliferating cells, CDC6...... is targeted for ubiquitin-mediated proteolysis by the anaphase promoting complex (APC)/cyclosome in G(1). A combination of point mutations in the destruction box and KEN-box motifs in CDC6 stabilizes the protein in G(1) and in quiescent cells. Furthermore, APC, in association with CDH1, ubiquitinates CDC6...... in vitro, and both APC and CDH1 are required and limiting for CDC6 proteolysis in vivo. Although a stable mutant of CDC6 is biologically active, overexpression of this mutant or wild-type CDC6 is not sufficient to induce multiple rounds of DNA replication in the same cell cycle. The APC-CDH1-dependent...

  1. Sistema de coberta transitable ventilada

    OpenAIRE

    Pagés Serra, Jorge

    2013-01-01

    Investigació referent a l'aplicació pràctica d'un sistema constructiu per a realitzar una coberta ventilada transitable amb paviment continuu En el nostre clima la màxima insolació es produeix a la coberta. Per altre banda les cobertes a la catalana o cobertes ventilades, tant comuns a l'eixample i d'altres zones de barcelona han pràcticament desaparegut. Aquesta investigació preten desenvolupar un sistema constructiu que permeti la realització de cobertes ventilades amb paviments continus...

  2. Sistemas de control moderno volumen II: sistemas de tiempo discreto

    OpenAIRE

    Fernández del Busto, Ricardo; Ramírez Mendoza, Ricardo A.

    2014-01-01

    El objetivo del eBook es iniciar al estudiante en los conceptos de los sistemas de control en tiempo discreto de tal manera que el alumno pueda entender los temas y complementarlos con los contenidos de libros de texto clásicos, ya sea para autoestudio o desarrollo de tareas.

  3. Sistemas de control moderno. Volumen I: sistemas de tiempo continuo

    OpenAIRE

    Morales Menéndez, Rubén; Ramírez Mendoza, Ricardo Ambrosio

    2013-01-01

    Este eBook pretende evidenciar la relevancia que tiene el control automático, así como facilitar la adquicisión de habilidades de análisis y síntesis en la identificación y la solución de problemas de los sistemas de control automático.

  4. Legislativo e política comercial: a aprovação do TLC com os Estados Unidos nos legislativos sul-americanos

    OpenAIRE

    Pedro Feliú Ribeiro

    2012-01-01

    O artigo analisa as votações nominais de três poderes legislativos sul-americanos, Chile, Colômbia e Peru, acerca da ratificação do Tratado de Livre Comércio com os Estados Unidos. A pergunta central é: quais são os fatores determinantes do voto do legislador na aprovação do Tratado de Livre Comércio (TLC) com os Estados Unidos? Três fatores centrais emergem da literatura: (i) as relações Estados Unidos x América Latina produzem uma clivagem ideológica (direita-esquerda) entre os partidos pol...

  5. Sistema de incendios inalámbrico

    OpenAIRE

    Menéndez Muñiz, Sergio

    2012-01-01

    Sistema de detección de incendios inalámbrico basado en dispositivos "motes" para la monitorización del estado del sistema, detección y reconocimiento de alarmas. Sistema de detecció d'incendis sense fils basat en dispositius "motes" per a la monitorització de l'estat del sistema, detecció i reconeixement d'alarmes.

  6. Androgens upregulate Cdc25C protein by inhibiting its proteasomal and lysosomal degradation pathways.

    Directory of Open Access Journals (Sweden)

    Yu-Wei Chou

    Full Text Available Cdc25C is a cell cycle protein of the dual specificity phosphatase family essential for activating the cdk1/Cyclin B1 complex in cells entering into mitosis. Since altered cell cycle is a hallmark of human cancers, we investigated androgen regulation of Cdc25C protein in human prostate cancer (PCa cells, including androgen-sensitive (AS LNCaP C-33 cells and androgen-independent (AI LNCaP C-81 as well as PC-3 cells. In the regular culture condition containing fetal bovine serum (FBS, Cdc25C protein levels were similar in these PCa cells. In a steroid-reduced condition, Cdc25C protein was greatly decreased in AS C-33 cells but not AI C-81 or PC-3 cells. In androgen-treated C-33 cells, the Cdc25C protein level was greatly elevated, following a dose- and a time-dependent manner, correlating with increased cell proliferation. This androgen effect was blocked by Casodex, an androgen receptor blocker. Nevertheless, epidermal growth factor (EGF, a growth stimulator of PCa cells, could only increase Cdc25C protein level by about 1.5-fold. Altered expression of Cdc25C in C-33 cells and PC-3 cells by cDNA and/or shRNA transfection is associated with the corresponding changes of cell growth and Cyclin B1 protein level. Actinomycin D and cycloheximide could only partially block androgen-induced Cdc25C protein level. Treatments with both proteasomal and lysosomal inhibitors resulted in elevated Cdc25C protein levels. Immunoprecipitation revealed that androgens reduced the ubiquitination of Cdc25C proteins. These results show for the first time that Cdc25C protein plays a role in regulating PCa cell growth, and androgen treatments, but not EGF, greatly increase Cdc25C protein levels in AS PCa cells, which is in part by decreasing its degradation. These results can lead to advanced PCa therapy via up-regulating the degradation pathways of Cdc25C protein.

  7. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry

    DEFF Research Database (Denmark)

    Hein, Jamin B; Nilsson, Jakob

    2016-01-01

    Proper cell-cycle progression requires tight temporal control of the Anaphase Promoting Complex/Cyclosome (APC/C), a large ubiquitin ligase that is activated by one of two co-activators, Cdh1 or Cdc20. APC/C and Cdc20 are already present during interphase but APC/C-Cdc20 regulation during...

  8. Knowledge Exchange with Sistema Scotland

    Science.gov (United States)

    Allan, Julie; Moran, Nikki; Duffy, Celia; Loening, Gica

    2010-01-01

    This paper reports on a knowledge exchange project, funded by the Scottish Funding Council and undertaken by a group of researchers from three higher education institutions in Scotland and the project partner, Sistema Scotland. This newly established charity is attempting to implement a major programme of social change, developed in Venezuela,…

  9. Thoughts on Seeing "El Sistema"

    Science.gov (United States)

    Booth, Eric

    2009-01-01

    The FESNOJIV (the Spanish acronym for the State Foundation for the National System of Youth and Children's Orchestras), more commonly known as "El Sistema," teaches 300,000 of Venezuela's poorest children in the nationwide music learning program. Many begin attending the "nucleo" as early as age two or three, and the vast…

  10. Cdc25A localisation and shuttling: characterisation of sequences mediating nuclear export and import

    International Nuclear Information System (INIS)

    Kaellstroem, Helena; Lindqvist, Arne; Pospisil, Vitek; Lundgren, Andreas; Karlsson Rosenthal, Christina

    2005-01-01

    The Cdc25 phosphatases play crucial roles in cell cycle progression by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases. Cdc25A is an important regulator of the G1/S transition but functions also in the mitotic phase of the human cell cycle. In this paper, we investigate the sub-cellular localisation of exogenously expressed Cdc25A. We show that YFP-Cdc25A is localised both in the nucleus and the cytoplasm of HeLa cells and untransformed fibroblasts. Cell fusion assays and fluorescence loss in photobleaching (FLIP) assays reveal that the localisation is dynamic and the protein shuttles between the nucleus and the cytoplasm. We demonstrate that nuclear export of Cdc25A is partly mediated by an N-terminal nuclear export sequence (NES), in a manner not sensitive to the Exportin 1-inhibitor leptomycin B. A nuclear localisation signal (NLS) is also characterised, mutation of which leads to cytoplasmic localisation of Cdc25A. Our results imply that the Cdc25A phosphatase may interact with substrates and regulators both in the nucleus and the cytoplasm

  11. Study of the docking-dependent PLK1 phosphorylation of the CDC25B phosphatase

    Energy Technology Data Exchange (ETDEWEB)

    Lobjois, Valerie [Universite de Toulouse, LBCMCP, F-31062 Toulouse (France); CNRS, ITAV-UMS3039, F-31106 Toulouse (France); Froment, Carine [CNRS, IPBS-UMR5089, F-31077 Toulouse (France); Universite de Toulouse, UPS, IPBS, F-31077 Toulouse (France); Braud, Emmanuelle [INSERM U648, F-75270 Paris Cedex 06 (France); Universite Paris Descartes, F-75270 Paris Cedex 06 (France); Grimal, Fanny [INSERM, CPTP-U563, F-31024 Toulouse (France); Burlet-Schiltz, Odile [CNRS, IPBS-UMR5089, F-31077 Toulouse (France); Universite de Toulouse, UPS, IPBS, F-31077 Toulouse (France); Ducommun, Bernard, E-mail: bernard.ducommun@itav-recherche.fr [Universite de Toulouse, LBCMCP, F-31062 Toulouse (France); CNRS, ITAV-UMS3039, F-31106 Toulouse (France); CHU de Toulouse, F-31059 Toulouse (France); Bouche, Jean-Pierre [Universite de Toulouse, LBCMCP, F-31062 Toulouse (France)

    2011-06-24

    Highlights: {yields} Phosphorylation of CDC25B by CDK1 enhances its substrate properties for PLK1 in vitro. {yields} Sequential phosphorylation of CDC25B is analyzed using {sup 16}O and {sup 18}O ATP. {yields} Thirteen sites phosphorylated by PLK1 have been identified. -- Abstract: CDC25 (A, B and C) phosphatases control cell cycle progression through the timely dephosphorylation and activation of cyclin-dependent kinases (CDK). At mitosis the CDC25B phosphatase activity is dependent on its phosphorylation by multiple kinases impinging on its localisation, stability and catalytic activity. Here we report that prior phosphorylation of CDC25B by CDK1 enhances its substrate properties for PLK1 in vitro, and we also show that phosphorylated S50 serves as a docking site for PLK1. Using a sophisticated strategy based on the sequential phosphorylation of CDC25B with {sup 16}O and {sup 18}O ATP prior to nanoLC-MS/MS analysis we identified 13 sites phosphorylated by PLK1. This study illustrates the complexity of the phosphorylation pattern and of the subsequent regulation of CDC25B activity.

  12. Identification of CDC25 as a Common Therapeutic Target for Triple-Negative Breast Cancer

    Directory of Open Access Journals (Sweden)

    Jeff C. Liu

    2018-04-01

    Full Text Available Summary: CDK4/6 inhibitors are effective against cancer cells expressing the tumor suppressor RB1, but not RB1-deficient cells, posing the challenge of how to target RB1 loss. In triple-negative breast cancer (TNBC, RB1 and PTEN are frequently inactivated together with TP53. We performed kinome/phosphatase inhibitor screens on primary mouse Rb/p53-, Pten/p53-, and human RB1/PTEN/TP53-deficient TNBC cell lines and identified CDC25 phosphatase as a common target. Pharmacological or genetic inhibition of CDC25 suppressed growth of RB1-deficient TNBC cells that are resistant to combined CDK4/6 plus CDK2 inhibition. Minimal cooperation was observed in vitro between CDC25 antagonists and CDK1, CDK2, or CDK4/6 inhibitors, but strong synergy with WEE1 inhibition was apparent. In accordance with increased PI3K signaling following long-term CDC25 inhibition, CDC25 and PI3K inhibitors effectively synergized to suppress TNBC growth both in vitro and in xenotransplantation models. These results provide a rationale for the development of CDC25-based therapies for diverse RB1/PTEN/TP53-deficient and -proficient TNBCs. : Liu et al. report that inhibition of the protein phosphatase CDC25 kills diverse triple-negative breast cancer (TNBC cells. Moreover, CDC25 antagonists cooperate with other drugs, such as PI3K inhibitors, to efficiently suppress growth of human TNBC engrafted into mice. Keywords: triple negative breast cancer, basal-like breast cancer, therapy, RB1, PTEN, TP53, CDC25, WEE1, CHK1, checkpoint control

  13. Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei

    International Nuclear Information System (INIS)

    Peng, Ting; Wang, Wei-Na; Gu, Mei-Mei; Xie, Chen-Ying; Xiao, Yu-Chao; Liu, Yuan; Wang, Lei

    2015-01-01

    Highlights: • Cd 2+ induces Cdc42 and MAPKs pathway related gene of Litopenaeus vannamei up-regulation. • Reduction of THC, increase of ROS production and apoptotic cell rate were observed when the shrimps exposure to Cd 2+ . • DsRNA-suppression of LvCdc42 and MAPKs during Cd 2+ stress reduces the ROS production and apoptosis. • We conclude that LvCdc42 and MAPKs play key roles in Cd 2+ stress responses of shrimps. - Abstract: Cadmium, one of the most toxic heavy metals in aquatic environments, has severe effects on marine invertebrates and fishes. The MAPK signaling pathway plays a vital role in stress responses of animals. The mitogen-activated protein kinase (MAPK) signaling pathway plays a vital role in animals’ stress responses, including mediation of apoptosis induced by the Rho GTPase Cdc42. However, there is limited knowledge about its function in shrimps, although disorders exacerbated by environmental stresses (including heavy metal pollution) have caused serious mortality in commercially cultured shrimps. Thus, we probed roles of Cdc42 in Litopenaeus vannamei shrimps (LvCdc42) during cadmium exposure by inhibiting its expression using dsRNA-mediated RNA interference. The treatment successfully reduced expression levels of MAPKs (including p38, JNK, and ERK). Cadmium exposure induced significant increases in expression levels of LvCdc42 and MAPKs, accompanied by reductions in total hemocyte counts (THC) and increases in apoptotic hemocyte ratios and ROS production. However, all of these responses were much weaker in LvCdc42-suppressed shrimps, in which mortality rates were higher than in controls. Our results suggest that the MAPK pathway plays a vital role in shrimps’ responses to Cd 2+ . They also indicate that LvCdc42 in shrimps participates in its regulation, and thus plays key roles in ROS production, regulation of apoptosis and associated stress responses

  14. Differential Regulation of G1 CDK Complexes by the Hsp90-Cdc37 Chaperone System

    Directory of Open Access Journals (Sweden)

    Stephen T. Hallett

    2017-10-01

    Full Text Available Summary: Selective recruitment of protein kinases to the Hsp90 system is mediated by the adaptor co-chaperone Cdc37. We show that assembly of CDK4 and CDK6 into protein complexes is differentially regulated by the Cdc37-Hsp90 system. Like other Hsp90 kinase clients, binding of CDK4/6 to Cdc37 is blocked by ATP-competitive inhibitors. Cdc37-Hsp90 relinquishes CDK6 to D3- and virus-type cyclins and to INK family CDK inhibitors, whereas CDK4 is relinquished to INKs but less readily to cyclins. p21CIP1 and p27KIP1 CDK inhibitors are less potent than the INKs at displacing CDK4 and CDK6 from Cdc37. However, they cooperate with the D-type cyclins to generate CDK4/6-containing ternary complexes that are resistant to cyclin D displacement by Cdc37, suggesting a molecular mechanism to explain the assembly factor activity ascribed to CIP/KIP family members. Overall, our data reveal multiple mechanisms whereby the Hsp90 system may control formation of CDK4- and CDK6-cyclin complexes under different cellular conditions. : Hallett et al. reconstitute CDK4/6 client kinase handover from Cdc37-Hsp90 to CDK regulatory partners and propose a model for the assembly factor activity of CIP/KIP CDK inhibitors. They find that CDK4/6 inhibitors in clinical use can displace G1 CDKs from the Cdc37-Hsp90 chaperone system at submicromolar concentrations. Keywords: Cdc37, CDK, chaperone, CIP/KIP, cyclin D, Hsp90, INK, kinase, palbociclib, ribociclib

  15. Two nuclear export signals of Cdc6 are differentially associated with CDK-mediated phosphorylation residues for cytoplasmic translocation.

    Science.gov (United States)

    Hwang, In Sun; Woo, Sang Uk; Park, Ji-Woong; Lee, Seung Ki; Yim, Hyungshin

    2014-02-01

    Cdc6 is cleaved at residues 442 and 290 by caspase-3 during apoptosis producing p49-tCdc6 and p32-tCdc6, respectively. While p32-tCdc6 is unable to translocate into the cytoplasm, p49-tCdc6 retains cytoplasmic translocation activity, but it has a lower efficiency than wild-type Cdc6. We hypothesized that a novel nuclear export signal (NES) sequence exists between amino acids 290 and 442. Cdc6 contains a novel NES in the region of amino acids 300-315 (NES2) that shares sequence similarity with NES1 at residues 462-476. In mutant versions of Cdc6, we replaced leucine with alanine in NES1 and NES2 and co-expressed the mutant constructs with cyclin A. We observed that the cytoplasmic translocation of these mutants was reduced in comparison to wild-type Cdc6. Moreover, the cytoplasmic translocation of a mutant in which all four leucine residues were mutated to alanine was significantly inhibited in comparison to the translocation of wild-type Cdc6. The Crm1 binding activities of Cdc6 NES mutants were consistent with the efficiency of its cytoplasmic translocation. Further studies have revealed that L468 and L470 of NES1 are required for cytoplasmic translocation of Cdc6 phosphorylated at S74, while L311 and L313 of NES2 accelerate the cytoplasmic translocation of Cdc6 phosphorylated at S54. These results suggest that the two NESs of Cdc6 work cooperatively and distinctly for the cytoplasmic translocation of Cdc6 phosphorylated at S74 and S54 by cyclin A/Cdk2. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Relaciones entre el derecho internacional y el derecho interno: Nuevas perspectivas doctrinales y jurisprudenciales en el ámbito americano

    Directory of Open Access Journals (Sweden)

    Aníbal Quiroga León

    2005-01-01

    Full Text Available El artículo analiza la protección de los derechos fundamentales en el marco de las relaciones entre derecho internacional y derecho interno, teniendo como punto central el sistema interamericano y sus órganos de aplicación, La Comisión y la Corte Interamericana de Derechos Humanos, considerando aspectos procedimentales y la adopción de medidas cautelares por esta última. Finalmente, se analiza la superioridad del derecho internacional de los derechos humanos sobre el derecho interno, teniendo como fundamento los casos "Barrios Altos" (Chumbipoma Aguirre y otros, cuya sentencia es del 14 de marzo del 2001 y el caso "La última tentación de Cristo" (Olmedo Bustos y otros cuya sentencia es del 05 de febrero del 2001.This article analyzes the protection of fundamental rights within the framework of the relationship between international and internal law. The core of our analysis is the Inter-American system, and its bodies of application: the Inter- American Human Rights Commission and Court. We consider procedural dimensions as well as precautionary measures pertaining to the latter. Finally, we examine the preeminence of the international law of human rights over internal law, on grounds of the "Barrios Altos" case (Chumbipoma Aguirre et al., decided on March, 2001 y the "La última tentación de Cristo" case (Olmedo Bustos et al. decided on February 5, 2001.

  17. NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling

    DEFF Research Database (Denmark)

    Sørensen, Claus Storgaard; Melixetian, Marina; Klein, Ditte Kjaersgaard

    2010-01-01

    The DNA damage induced G(2)/M checkpoint is an important guardian of the genome that prevents cell division when DNA lesions are present. The checkpoint prevents cells from entering mitosis by degrading CDC25A, a key CDK activator. CDC25A proteolysis is controlled by direct phosphorylation events...... is required for beta-TrCP mediated CDC25A polyubiquitylation and degradation. The activity of NEK11 is in turn controlled by CHK1 that activates NEK11 via phosphorylation on serine 273. Since inhibition of NEK11 activity forces checkpoint-arrested cells into mitosis and cell death, NEK11 is, like CHK1...

  18. Cdc20 mediates D-box-dependent degradation of Sp100

    International Nuclear Information System (INIS)

    Wang, Ran; Li, Ke-min; Zhou, Cai-hong; Xue, Jing-lun; Ji, Chao-neng; Chen, Jin-zhong

    2011-01-01

    Highlights: ► Cdc20 is a co-activator of APC/C complex. ► Cdc20 recruits Sp100 and mediates its degradation. ► The D-box of Sp100 is required for Cdc20-mediated degradation. ► Sp100 expresses consistently at both the mRNA and protein levels in cell cycle. -- Abstract: Cdc20 is a co-activator of the anaphase-promoting complex/cyclosome (APC/C complex), which recruits substrates at particular phases of the cell cycle and mediates their degradation. Sp100 is a PML-NB scaffold protein, which localizes to nuclear particles during interphase and disperses from them during mitosis, participates in viral resistance, transcriptional regulation, and apoptosis. However, its metabolism during the cell cycle has not yet been fully characterized. We found a putative D-box in Sp100 using the Eukaryotic Linear Motif (ELM) predictor database. The putative D-box of Sp100 was verified by mutational analysis. Overexpression of Cdc20 resulted in decreased levels of both endogenous Sp100 protein and overexpressed Sp100 mRNA in HEK 293 cells. Only an overexpressed D-box deletion mutant of Sp100 accumulated in HEK293 cells that also overexpressed Cdc20. Cdc20 knockdown by cdc20 specific siRNA resulted in increased Sp100 protein levels in cells. Furthermore, we discovered that the Cdc20 mediated degradation of Sp100 is diminished by the proteasome inhibitor MG132, which suggests that the ubiquitination pathway is involved in this process. However, unlike the other Cdc20 substrates, which display oscillating protein levels, the level of Sp100 protein remains constant throughout the cell cycle. Additionally, both overexpression and knockdown of endogenous Sp100 had no effect on the cell cycle. Our results suggested that sp100 is a novel substrate of Cdc20 and it is degraded by the ubiquitination pathway. The intact D-box of Sp100 was necessary for this process. These findings expand our knowledge of both Sp100 and Cdc20 as well as their role in ubiquitination.

  19. Cooperation of Rho family proteins Rac1 and Cdc42 in cartilage development and calcified tissue formation.

    Science.gov (United States)

    Ikehata, Mikiko; Yamada, Atsushi; Fujita, Koji; Yoshida, Yuko; Kato, Tadashi; Sakashita, Akiko; Ogata, Hiroaki; Iijima, Takehiko; Kuroda, Masahiko; Chikazu, Daichi; Kamijo, Ryutaro

    2018-04-20

    Rac1 and Cdc42, Rho family low molecular weight G proteins, are intracellular signaling factors that transmit various information from outside to inside cells. Primarily, they are known to control various biological activities mediated by actin cytoskeleton reorganization, such as cell proliferation, differentiation, and apoptosis. In order to investigate the functions of Rac1 and Cdc42 in bone formation, we prepared cartilage-specific double conditional knockout mice, Rac1 fl/fl ; Cdc42 fl/fl ; Col2-Cre (Rac1: Cdc42 dcKO mice), which died just after birth, similar to Cdc42 fl/fl ; Col2-Cre mice (Cdc42 cKO mice). Our findings showed that the long tubule bone in Rac1: Cdc42 dcKO mice was shorter than that in Rac1 fl/fl ; Col2-Cre mice (Rac1 cKO mice) and Cdc42 cKO mice. Abnormal skeleton formation was also observed and disordered columnar formation in the growth plate of the Rac1: Cdc42 dcKO mice was more severe as compared to the Rac1 cKO and Cdc42 cKO mice. Together, these results suggest that Rac1 and Cdc42 have cooperating roles in regulation of bone development. Copyright © 2018 Elsevier Inc. All rights reserved.

  20. Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells

    DEFF Research Database (Denmark)

    Czuchra, Aleksandra; Wu, Xunwei; Meyer, Hannelore

    2005-01-01

    Cdc42 is a small GTPase involved in the regulation of the cytoskeleton and cell polarity. To test whether Cdc42 has an essential role in the formation of filopodia or directed cell migration, we generated Cdc42-deficient fibroblastoid cells by conditional gene inactivation. We report here that loss...... of Cdc42 did not affect filopodium or lamellipodium formation and had no significant influence on the speed of directed migration nor on mitosis. Cdc42-deficient cells displayed a more elongated cell shape and had a reduced area. Furthermore, directionality during migration and reorientation of the Golgi...... apparatus into the direction of migration was decreased. However, expression of dominant negative Cdc42 in Cdc42-null cells resulted in strongly reduced directed migration, severely reduced single cell directionality, and complete loss of Golgi polarization and of directionality of protrusion formation...

  1. Characterization of a Dual CDC7/CDK9 Inhibitor in Multiple Myeloma Cellular Models

    International Nuclear Information System (INIS)

    Natoni, Alessandro; Coyne, Mark R. E.; Jacobsen, Alan; Rainey, Michael D.; O’Brien, Gemma; Healy, Sandra; Montagnoli, Alessia; Moll, Jürgen; O’Dwyer, Michael; Santocanale, Corrado

    2013-01-01

    Two key features of myeloma cells are the deregulation of the cell cycle and the dependency on the expression of the BCL2 family of anti-apoptotic proteins. The cell division cycle 7 (CDC7) is an essential S-phase kinase and emerging CDC7 inhibitors are effective in a variety of preclinical cancer models. These compounds also inhibit CDK9 which is relevant for MCL-1 expression. The activity and mechanism of action of the dual CDC7/CDK9 inhibitor PHA-767491 was assessed in a panel of multiple myeloma cell lines, in primary samples from patients, in the presence of stromal cells and in combination with drugs used in current chemotherapeutic regimens. We report that in all conditions myeloma cells undergo cell death upon PHA-767491 treatment and we report an overall additive effect with melphalan, bortezomib and doxorubicin, thus supporting further assessment of targeting CDC7 and CDK9 in multiple myeloma

  2. Characterization of a Dual CDC7/CDK9 Inhibitor in Multiple Myeloma Cellular Models

    Energy Technology Data Exchange (ETDEWEB)

    Natoni, Alessandro [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Coyne, Mark R. E. [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Department of Medicine, National University of Ireland Galway, Galway (Ireland); Department of Haematology, Galway University Hospital, Galway (Ireland); Jacobsen, Alan; Rainey, Michael D.; O’Brien, Gemma; Healy, Sandra [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Montagnoli, Alessia; Moll, Jürgen [Nerviano Medical Sciences S.r.l., Via Pasteur 10, Nerviano 20014 (Italy); O’Dwyer, Michael, E-mail: michael.odwyer@nuigalway.ie [Department of Medicine, National University of Ireland Galway, Galway (Ireland); Department of Haematology, Galway University Hospital, Galway (Ireland); Santocanale, Corrado, E-mail: michael.odwyer@nuigalway.ie [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland)

    2013-07-24

    Two key features of myeloma cells are the deregulation of the cell cycle and the dependency on the expression of the BCL2 family of anti-apoptotic proteins. The cell division cycle 7 (CDC7) is an essential S-phase kinase and emerging CDC7 inhibitors are effective in a variety of preclinical cancer models. These compounds also inhibit CDK9 which is relevant for MCL-1 expression. The activity and mechanism of action of the dual CDC7/CDK9 inhibitor PHA-767491 was assessed in a panel of multiple myeloma cell lines, in primary samples from patients, in the presence of stromal cells and in combination with drugs used in current chemotherapeutic regimens. We report that in all conditions myeloma cells undergo cell death upon PHA-767491 treatment and we report an overall additive effect with melphalan, bortezomib and doxorubicin, thus supporting further assessment of targeting CDC7 and CDK9 in multiple myeloma.

  3. Defective tubulin organization and proplatelet formation in murine megakaryocytes lacking Rac1 and Cdc42

    DEFF Research Database (Denmark)

    Pleines, Irina; Dütting, Sebastian; Cherpokova, Deya

    2013-01-01

    and tubulin cytoskeleton. Rho GTPases, such as RhoA, Rac1, and Cdc42, are important regulators of cytoskeletal rearrangements in platelets; however, the specific roles of these proteins during platelet production have not been established. Using conditional knockout mice, we show here that Rac1 and Cdc42...... possess redundant functions in platelet production and function. In contrast to a single-deficiency of either protein, a double-deficiency of Rac1 and Cdc42 in MKs resulted in macrothrombocytopenia, abnormal platelet morphology, and impaired platelet function. Double-deficient bone marrow MKs matured...... normally in vivo but displayed highly abnormal morphology and uncontrolled fragmentation. Consistently, a lack of Rac1/Cdc42 virtually abrogated proplatelet formation in vitro. Strikingly, this phenotype was associated with severely defective tubulin organization, whereas actin assembly and structure were...

  4. CDC Vital Signs: Making Health Care Safer -- Think Sepsis. Time Matters.

    Science.gov (United States)

    ... germs that can cause sepsis are Staphylococcus aureus, Escherichia coli (E. coli) , and some types of Streptococcus . *Among ... RSS VitalSigns RSS Related Links Download this factsheet [PDF – 1.75 MB] CDC Digital Press Kit Read ...

  5. CDC Vital Signs: Making Health Care Safer -- Protect Patients from Antibiotic Resistance

    Science.gov (United States)

    ... About CDC.gov . Vital Signs Topics Covered Alcohol Antibiotic Resistance Cancer Cardiovascular Diseases Diseases & Conditions Food Safety Healthcare- ... Clips Making Health Care Safer Protect patients from antibiotic resistance Language: English (US) Español (Spanish) Recommend on Facebook ...

  6. CDC Vital Signs: Progress on Children Eating More Fruit, Not Vegetables

    Science.gov (United States)

    ... programs such as the National School Lunch and Breakfast Programs. State and local officials can Include nutrition ... MB] CDC Guide to Strategies to Increase the Consumption of Fruits and Vegetables [PDF – 2.1 MB] ...

  7. SIMWEST: A simulation model for wind and photovoltaic energy storage systems (CDC user's manual), volume 1

    Science.gov (United States)

    Warren, A. W.; Esinger, A. W.

    1979-01-01

    Procedures are given for using the SIMWEST program on CDC 6000 series computers. This expanded software package includes wind and/or photovoltaic systems utilizing any combination of five types of storage (pumped hydro, battery, thermal, flywheel, and pneumatic).

  8. Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells

    Directory of Open Access Journals (Sweden)

    Renzheng Huang

    2017-04-01

    Full Text Available Pre-mRNA processing factor 19 (Prp19 is involved in many cellular events including pre-mRNA processing and DNA damage response. Recently, it has been identified as a candidate oncogene in hepatocellular carcinoma (HCC. However, the role of Prp19 in tumor biology is still elusive. Here, we reported that Prp19 arrested cell cycle in HCC cells via regulating G2/M transition. Mechanistic insights revealed that silencing Prp19 inhibited the expression of cell division cycle 5-like (Cdc5L via repressing the translation of Cdc5L mRNA and facilitating lysosome-mediated degradation of Cdc5L in HCC cells. Furthermore, we found that silencing Prp19 induced cell cycle arrest could be partially resumed by overexpressing Cdc5L. This work implied that Prp19 participated in mitotic progression and thus could be a promising therapeutic target of HCC.

  9. Working at a Ferranti-Argus graphics display linked to a CDC 6600

    CERN Multimedia

    CERN PhotoLab

    1975-01-01

    The photo shows a Ferranti-Argus graphics display linked to a CDC 6600 computer running one of the first interactive graphics packages for physics analysis. The people around are Jean-Claude Marin, Harry Renshall and Emilio Pagiola (right).

  10. Frequent alterations of SLIT2–ROBO1–CDC42 signalling pathway ...

    Indian Academy of Sciences (India)

    2016-09-07

    CDC42 signalling pathways in development of breast cancer (BC). Primary BC samples (n = 150), comprising of almost equal proportion of four subtypes were tested for molecu- lar alterations of SLIT2, ROBO1, ROBO2 and ...

  11. Communications and Web services: What do CDC users desire in partner relationship management and does CDC's PHIN Directory meet the need?

    Science.gov (United States)

    Cervone, Maria A; Savel, Thomas G

    2006-01-01

    The National Center on Birth Defects and Developmental Disabilities (NCBDDD) at the Centers for Disease Control and Prevention (CDC) sought to establish a database to proactively manage their partner relationships with external organizations. A user needs analysis was conducted, and CDC's Public Health Information Network Directory (PHINDIR) was evaluated as a possible solution. PHINDIR could sufficiently maintain contact information but did not address customer relationships; however, its flexible architecture allows add-on applications via web services. Thus, NCBDDD's needs could be met via PHINDIR.

  12. Sistema Kaizen en la administracion

    Directory of Open Access Journals (Sweden)

    Gallegos, H.

    2007-01-01

    Full Text Available En este artículo presentaremos la importancia que tiene el área de administración como factor de reducción de costos para la empresa aplicando el sistema kaizen. Analizaremos en forma general la administración de las empresas, enfocándonos en las áreas de oportunidad que pueden existir, así como la posible solución a esto, utilizando el sistema kaizen, obteniendo como resultado una reducción de costos, tiempos o mejoras en los procesos administrativos. También se anexa un caso practico en el cual se muestra como aplicando la metodología kaizen en las actividades administrativas podemos lograr una reducción de tiempos y/o costos todo esto causado por la reingeniería de los procesos.

  13. Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization

    DEFF Research Database (Denmark)

    Petersen, B O; Lukas, J; Sørensen, Claus Storgaard

    1999-01-01

    by CDKs. CDC6 interacts specifically with the active Cyclin A/CDK2 complex in vitro and in vivo, but not with Cyclin E or Cyclin B kinase complexes. The cyclin binding domain of CDC6 was mapped to an N-terminal Cy-motif that is similar to the cyclin binding regions in p21(WAF1/SDI1) and E2F-1. The in vivo...

  14. CDC Vital Signs–Legionnaires’ Disease

    Centers for Disease Control (CDC) Podcasts

    2017-06-06

    This podcast is based on the June 2017 CDC Vital Signs report. Legionnaires’ disease is a serious, often deadly lung infection. People most commonly get it by breathing in water droplets containing Legionella germs. Learn how to prevent infections from Legionella.  Created: 6/6/2017 by Centers for Disease Control and Prevention (CDC).   Date Released: 6/6/2017.

  15. Centers for Disease Control and Prevention (CDC) Radiation Hazard Scale Data Product Review Feedback Report

    Energy Technology Data Exchange (ETDEWEB)

    Askin, A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Buddemeier, B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Alai, M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Yu, K. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2017-09-20

    In support of the Department of Energy (DOE) National nuclear Security Administration (NNSA) and the Centers for Disease Control and Prevention (CDC), Lawrence Livermore National Laboratory (LLNL) assisted in the development of new data templates for disseminating and communicating FRMAC1 data products using the CDC Radiation Hazard Scale communication tool. To ensure these data products will be useful to stakeholders during a radiological emergency, LLNL facilitated opportunities for product socialization and review.

  16. Sistemas de control en estructuras

    Directory of Open Access Journals (Sweden)

    José Luis Ahumada Villafañe

    2010-01-01

    Full Text Available En el diseño estructural, la capacidad de disipación de energía en elementos que conforman los sistemas resistentes a fuerzas dinámicas, como las impartidas por la actividad sísmica y eólica, es suministrada por un minucioso detallado del acero de refuerzo y dimensiones en las secciones transversales de los elementos. Esta práctica es realizada bajo muchas suposiciones que en algunas ocasiones no corresponden a la realidad, y como es de esperarse, producen resultados inexactos y un desconocimiento del desempeño de la estructura. Por lo anterior es riesgoso confiar el 100% de la capacidad estructural a los elementos sobre todo cuando conforman sistemas estructurales diseñados en zonas de amenaza sísmica alta. En este artículo se presentan los sistemas de control, los cuales son una serie de dispositivos adaptados a las estructuras que absorben gran parte de la energía sísmica y liberan los elementos estructurales en gran porcentaje de la acción sísmica, logrando disminuir en ellas las solicitaciones (fuerzas internas y las respuestas (derivas con el objetivo de conseguir un buen desempeño, limitar el daño y abaratar los costos de reparación de fallas localizadas. Los sistemas de control que se analizarán en el presente artículo se dividen en control pasivo, control activo, control semiactivo y control híbrido.

  17. O Crisol: periódico das alunas do Colégio Americano (Porto Alegre/RS, 1945-1964 - O Crisol: the jornal by students of the Colégio Americano (Porto Alegre/RS, 1945-1964

    Directory of Open Access Journals (Sweden)

    Dóris Bittencourt Almeida, Brasil

    2013-04-01

    Full Text Available Neste trabalho investiga-se o periódico O Crisol, produzido pelas alunas do Colégio Americano, uma escola de Porto Alegre/RS, entre os anos de 1945 e 1964. Esse periódicodiz muito à História da Educação, pois evidencia saberes e práticas que circulavam nacomunidade do Colégio e revela um pouco das representações da cultura juvenil daquele tempo. Aqui interessam os significados dos discursos difundidos pelo periódico nos processos de subjetivação provocados pelos textos e suas possíveis influências na construção das identidades das jovens leitoras e escritoras do jornal. A pesquisa identifica-se com os pressupostos teóricos da História Cultural e se inscreve no campo das práticas de leitura e escrita e de memórias juvenis, tendo como referenciais as concepções da cultura escrita enquanto produção discursiva de um determinado tempo e lugar.Palavras-chave: memórias juvenis, escritas escolares, práticas de escrita. O CRISOL: THE JORNAL BY STUDENTS OF THE COLÉGIO AMERICANO (PORTO ALEGRE/RS, 1945-1964 AbstractThis work investigates the journalO Crisolproduced by students of the Colégio Americano, a school in Porto Alegre/RS, between the years 1945 and 1964.The journal says much to the History of Education, as evidenced knowledges and pratices circulating in the community,showing a bit of representations of youth culture in that time. Herethe interest by meaning’s dissemination of speeches by the journal in the processes of subjectivation caused by texts and their possible influences on the construction of identities of young readers and writers of the newspaper. The research identifies with the theoreticals presuppositions o Cultural History and inscribed in the field of reading and writing practices and memories of youth, taking as reference the concepts of the writing culture as a discursive production of a determinated time and place.Key-words: youth’s memories, written school, writing practices. O CRISOL: EL

  18. ATX-3, CDC-48 and UBXN-5: a new trimolecular complex in Caenorhabditis elegans.

    Science.gov (United States)

    Rodrigues, Ana-João; Neves-Carvalho, Andreia; Ferro, Anabela; Rokka, Anne; Corthals, Garry; Logarinho, Elsa; Maciel, Patrícia

    2009-09-04

    Ataxin-3 is the protein involved in Machado-Joseph disease, a neurodegenerative disorder caused by a polyglutamine expansion. Ataxin-3 binds ubiquitylated proteins and acts as a deubiquitylating enzyme in vitro. It was previously proposed that ataxin-3, along with the VCP/p97 protein, escorts ubiquitylated substrates for proteasomal degradation, although other players of this escort complex were not identified yet. In this work, we show that the Caenorhabditis elegans ataxin-3 protein (ATX-3) interacts with both VCP/p97 worm homologs, CDC-48.1 and CDC-48.2 and we map the interaction domains. We describe a motility defect in both ATX-3 and CDC-48.1 mutants and, in addition, we identify a new protein interactor, UBXN-5, potentially an adaptor of the CDC-48-ATX-3 escort complex. CDC-48 binds to both ATX-3 and UBXN-5 in a non-competitive manner, suggesting the formation of a trimolecular complex. Both CDC-48 and ATX-3, but not UBXN-5, were able to bind K-48 polyubiquitin chains, the standard signal for proteasomal degradation. Additionally, we describe several common interactors of ATX-3 and UBXN-5, some of which can be in vivo targets of this complex.

  19. Molecular Mechanism of Substrate Processing by the Cdc48 ATPase Complex.

    Science.gov (United States)

    Bodnar, Nicholas O; Rapoport, Tom A

    2017-05-04

    The Cdc48 ATPase and its cofactors Ufd1/Npl4 (UN) extract polyubiquitinated proteins from membranes or macromolecular complexes, but how they perform these functions is unclear. Cdc48 consists of an N-terminal domain that binds UN and two stacked hexameric ATPase rings (D1 and D2) surrounding a central pore. Here, we use purified components to elucidate how the Cdc48 complex processes substrates. After interaction of the polyubiquitin chain with UN, ATP hydrolysis by the D2 ring moves the polypeptide completely through the double ring, generating a pulling force on the substrate and causing its unfolding. ATP hydrolysis by the D1 ring is important for subsequent substrate release from the Cdc48 complex. This release requires cooperation of Cdc48 with a deubiquitinase, which trims polyubiquitin to an oligoubiquitin chain that is then also translocated through the pore. Together, these results lead to a new paradigm for the function of Cdc48 and its mammalian ortholog p97/VCP. Copyright © 2017 Elsevier Inc. All rights reserved.

  20. [Prokaryotic expression and histological localization of the Taenia solium CDC37 gene].

    Science.gov (United States)

    Huang, Jiang; Li, Bo; Dai, Jia-Lin; Zhang, Ai-Hua

    2013-02-01

    To express Taenia solium gene encoding cell division cycle 37 protein (TsCDC37) and investigate its antigenicity and localization in adults of Taenia solium. The complete coding sequence of TsCDC37 was amplified by PCR based on the recombinant plasmid clone from the cDNA library of adult Taenia solium. The PCR product was cloned into a prokaryotic expression vector pET-28a (+). The recombinant expression plasmid was identified by PCR, double endonuclease digestion and sequencing. The recombinant plasmid was transformed into E. coli BL21/DE3 and followed by expression of the protein induced by IPTG. The mice were immunized subcutaneously with purified recombinant TsCDC37 formulated in Freund's adjuvant. The antigenicity of the recombinant protein was examined by Western blotting. The localization of TsCDC37 in adult worms was demonstrated by immunofluorescent technique. The recombinant expression vector was constructed successfully. The recombinant protein was about M(r) 52 000, it was then purified and specifically recognized by immuno sera of SD rats and sera from patients infected with Taenia solium, Taenia saginata or Taenia asiatica. The immunofluorescence assay revealed that TsCDC37 located at the tegument of T. solium adult and the eggs. TsCDC37 gene has been expressed with immunoreactivity. The recombinant protein is mainly expressed in tegument and egg, and is a common antigen of the three human taenia cestodes.

  1. Functional interaction of human Cdc37 with the androgen receptor but not with the glucocorticoid receptor.

    Science.gov (United States)

    Rao, J; Lee, P; Benzeno, S; Cardozo, C; Albertus, J; Robins, D M; Caplan, A J

    2001-02-23

    Cdc37 is a molecular chaperone closely associated with the folding of protein kinases. Results from studies using a yeast model system showed that it was also important for activation of the human androgen receptor (AR). Based on results from the yeast model system (Fliss, A. E., Fang, Y., Boschelli, F., and Caplan, A. J. (1997) Mol. Biol. Cell 8, 2501-2509), we initiated studies to address whether AR and Cdc37 interact with each other in animal cell systems. Our results show that Cdc37 binds to AR but not to glucocorticoid receptors (GR) synthesized in rabbit reticulocyte lysates. This binding occurs via the ligand-binding domain of the AR in a manner that is partially dependent on Hsp90 and the presence of hormone. Further studies using the yeast system showed that Cdc37 is not interchangeable with Hsp90, suggesting that it functions at a distinct step in the activation pathway. Expression of a dominant negative form of Cdc37 in animal cells down-regulates full-length AR but has very little effect on an AR truncation lacking the ligand-binding domain or full-length GR. These results reveal differences in the mechanisms by which AR and GR become active transcription factors and strengthen the notion that Cdc37 has a wider range of polypeptide clients than was realized previously.

  2. Macroscopia do aparelho digestório do cágado sul-americano Mesoclemmys vanderhaegei (Bour, 1973

    Directory of Open Access Journals (Sweden)

    Juliana Normando Pinheiro

    2010-12-01

    Full Text Available Ainda há poucas descrições anatômicas a cerca do trato digestório de representantes da ordem Testudines, especialmente sobre o cágado-de-Vanderhaegei, Mesoclemmys vanderhaegei. A ocorrência desta espécie abrange as bacias dos rios Paraguai, Paraná e Amazônica. Estudos sobre a sua ecologia e morfologia ainda são pouco explorados, dessa forma, o aparelho digestório foi caracterizado macroscopicamente pela dissecção desse sistema em dez espécimes. A boca possui comprimento orocaudal ligeiramente maior que o laterolateral. Os lábios são formados por placas córneas. A língua é macia e está totalmente fixada ao assoalho da cavidade oral. O esôfago possui luz ampla e paredes delgadas na região cervical, tornando-se gradativamente mais estreito e de paredes espessas caudalmente. O estômago apresenta forma semelhante à letra "U" e podem ser distinguidas as regiões do cárdia, do corpo e do piloro, com mucosa totalmente pregueada. Na transição deste para o intestino delgado o esfíncter pilórico é conspícuo. O intestino apresenta-se disposto entre o delgado e grosso, sendo o primeiro constituído pelo duodeno e jejuno, não sendo possível identificar macroscopicamente o íleo. Da mesma forma, não se observa o ceco como primeiro segmento do intestino grosso, sendo este formado pelo cólon, que se abre em um pequeno compartimento pigmentado da cloaca. O fígado apresenta variação no padrão de lobação, no entanto, a presença dos lobos hepáticos direito e esquerdo é constante nos espécimes analisados. O pâncreas é facilmente observado em sintopia com o duodeno. Nesta análise, o trato digestório de Mesoclemmys vanderhaegei apresenta padrão semelhante ao de outras espécies de quelônios pesquisados da família Chelidae.

  3. A QUESTÃO DO ATRASO ECONÔMICO LATINO-AMERICANO: UMA ABORDAGEM TEÓRICA PARA A SUA DISCUSSÃO NO ÂMBITO DA ECONOMIA POLÍTICA INTERNACIONAL

    Directory of Open Access Journals (Sweden)

    Rodrigo Pereira de Castro

    2010-07-01

    Full Text Available Através deste estudo, procuramos apresentar o período entre as independências dos países americanos (final do século XVIII e início do XIX e a grande depressão mundial de 1929. Com base nos textos e discussões de diferentes autores, defensores de teses distintas, buscamos abordar as causas do atraso relativo das economias dos países latino-americanos e caribenhos em comparação com os Estados Unidos e o Canadá, exemplos de ex-colônias de metrópoles européias que sofreram processos de desenvolvimento diferentes das demais.

  4. Articles Published and Downloaded by Public Health Scientists: Analysis of Data From the CDC Public Health Library, 2011-2013.

    Science.gov (United States)

    Iskander, John; Bang, Gail; Stupp, Emma; Connick, Kathy; Gomez, Onnalee; Gidudu, Jane

    2016-01-01

    To describe scientific information usage and publication patterns of the Centers for Disease Control and Prevention (CDC) Public Health Library and Information Center patrons. Administratively collected patron usage data and aggregate data on CDC-authored publications from the CDC Library for 3 consecutive years were analyzed. The CDC Public Health Library and Information Center, which serves CDC employees nationally and internationally. Internal patrons and external users of the CDC Library. Three-year trends in full-text article publication and downloads including most common journals used for each purpose, systematic literature searches requested and completed, and subscriptions to a weekly public health current literature awareness service. From 2011 to 2013, CDC scientists published a total of 7718 articles in the peer-reviewed literature. During the same period, article downloads from the CDC Library increased 25% to more than 1.1 million, completed requests for reviews of the scientific literature increased by 34%, and electronic subscriptions to literature compilation services increased by 23%. CDC's scientific output and information use via the CDC Library are both increasing. Researchers and field staff are making greater use of literature review services and other customized information content delivery. Virtual public health library access is an increasingly important resource for the scientific practice of public health.

  5. The small GTPase Cdc42 modulates the number of exocytosis-competent dense-core vesicles in PC12 cells

    International Nuclear Information System (INIS)

    Sato, Mai; Kitaguchi, Tetsuya; Numano, Rika; Ikematsu, Kazuya; Kakeyama, Masaki; Murata, Masayuki; Sato, Ken; Tsuboi, Takashi

    2012-01-01

    Highlights: ► Regulation of exocytosis by Rho GTPase Cdc42. ► Cdc42 increases the number of fusion events from newly recruited vesicles. ► Cdc42 increases the number of exocytosis-competent dense-core vesicles. -- Abstract: Although the small GTPase Rho family Cdc42 has been shown to facilitate exocytosis through increasing the amount of hormones released, the precise mechanisms regulating the quantity of hormones released on exocytosis are not well understood. Here we show by live cell imaging analysis under TIRF microscope and immunocytochemical analysis under confocal microscope that Cdc42 modulated the number of fusion events and the number of dense-core vesicles produced in the cells. Overexpression of a wild-type or constitutively-active form of Cdc42 strongly facilitated high-KCl-induced exocytosis from the newly recruited plasma membrane vesicles in PC12 cells. By contrast, a dominant-negative form of Cdc42 inhibited exocytosis from both the newly recruited and previously docked plasma membrane vesicles. The number of intracellular dense-core vesicles was increased by the overexpression of both a wild-type and constitutively-active form of Cdc42. Consistently, activation of Cdc42 by overexpression of Tuba, a Golgi-associated guanine nucleotide exchange factor for Cdc42 increased the number of intracellular dense-core vesicles, whereas inhibition of Cdc42 by overexpression of the Cdc42/Rac interactive binding domain of neuronal Wiskott–Aldrich syndrome protein decreased the number of them. These findings suggest that Cdc42 facilitates exocytosis by modulating both the number of exocytosis-competent dense-core vesicles and the production of dense-core vesicles in PC12 cells.

  6. The small GTPase Cdc42 modulates the number of exocytosis-competent dense-core vesicles in PC12 cells

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Mai [Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902 (Japan); Kitaguchi, Tetsuya [Cell Signaling Group, Waseda Bioscience Research Institute in Singapore (WABOIS), Waseda University, 11 Biopolis Way, 05-01/02 Helios, Singapore 138667 (Singapore); Numano, Rika [The Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka, Tennpaku-cho, Toyohashi, Aichi 441-8580 (Japan); Ikematsu, Kazuya [Forensic Pathology and Science, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523 (Japan); Kakeyama, Masaki [Laboratory of Environmental Health Sciences, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan); Murata, Masayuki; Sato, Ken [Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902 (Japan); Tsuboi, Takashi, E-mail: takatsuboi@bio.c.u-tokyo.ac.jp [Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902 (Japan)

    2012-04-06

    Highlights: Black-Right-Pointing-Pointer Regulation of exocytosis by Rho GTPase Cdc42. Black-Right-Pointing-Pointer Cdc42 increases the number of fusion events from newly recruited vesicles. Black-Right-Pointing-Pointer Cdc42 increases the number of exocytosis-competent dense-core vesicles. -- Abstract: Although the small GTPase Rho family Cdc42 has been shown to facilitate exocytosis through increasing the amount of hormones released, the precise mechanisms regulating the quantity of hormones released on exocytosis are not well understood. Here we show by live cell imaging analysis under TIRF microscope and immunocytochemical analysis under confocal microscope that Cdc42 modulated the number of fusion events and the number of dense-core vesicles produced in the cells. Overexpression of a wild-type or constitutively-active form of Cdc42 strongly facilitated high-KCl-induced exocytosis from the newly recruited plasma membrane vesicles in PC12 cells. By contrast, a dominant-negative form of Cdc42 inhibited exocytosis from both the newly recruited and previously docked plasma membrane vesicles. The number of intracellular dense-core vesicles was increased by the overexpression of both a wild-type and constitutively-active form of Cdc42. Consistently, activation of Cdc42 by overexpression of Tuba, a Golgi-associated guanine nucleotide exchange factor for Cdc42 increased the number of intracellular dense-core vesicles, whereas inhibition of Cdc42 by overexpression of the Cdc42/Rac interactive binding domain of neuronal Wiskott-Aldrich syndrome protein decreased the number of them. These findings suggest that Cdc42 facilitates exocytosis by modulating both the number of exocytosis-competent dense-core vesicles and the production of dense-core vesicles in PC12 cells.

  7. The correct use of the vernacular names for South American dolphins genus Sotalia Gray (Mammalia, Cetacea, Delphinidae O uso correto de nomes vernaculares dos botos sul-americanos do gênero Sotalia Gray (Mammalia, Cetacea, Delphinidae

    Directory of Open Access Journals (Sweden)

    Lena Geise

    2006-12-01

    Full Text Available The usage of the vernacular names for South American dolphins genus Sotalia Gray, 1866 is discussed in accordance to geographic characteristics of local people identification.O uso de nomes vernaculares para os botos sul-americanos do gênero Sotalia Gray, 1866 são discutidos de acordo com as características geográficas das identificações feitas pelas populações litorâneas.

  8. Gerencia de sistemas conductuales y cambio organizacional

    OpenAIRE

    Guillermo E. Yáber; María E. Malott; Elizabeth Valarino

    1998-01-01

    La gerencia de sistemas conductuales es una tecnología basada en un enfoque científico para el análisis del comportamiento en sistemas humanos. Esta tecnología ayuda a las organizaciones a definir y lograr sus metas. Consiste en el análisis, especificación, diseño, implementación, evaluación y reciclaje de sistemas de motivación humana que optimizan el desempeño y desarrollo a nivel del sistema total, departamental e individual de la organización. Utiliza tres modelos: El di...

  9. Control de sistemas caóticos

    OpenAIRE

    RODRÍGUEZ LIÑÁN, JUAN ÁNGEL; DE LEÓN MORALES, JESÚS

    2008-01-01

    Se presenta una estrategia para control de caos (supresión y sincronización) en una clase de sistemas. Los sistemas estudiados, conocidos como clase P, son los más simples que exhiben caos. Se diseña un observador adaptable para una clase de sistemas afines en el estado, el cual se sincroniza con los sistemas clase P al estimar sus estados y parámetros desconocidos. Además, se considera una ley de control, dependiente de las estimaciones del observador, que permite...

  10. As contribuições do II Congresso Médico Latino-Americano para a enfermagem no Brasil Las Contribuciones del II Congreso Médico Latino-Americano para la enfermería en el Brasil The contributions of II Latin American Medical Congress for the Nursing in Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda Teles Morais

    2009-03-01

    Full Text Available Estudo sobre as recomendações do II Congresso Médico Latino-Americano, em 1904, para a organização da Enfermagem no Brasil. Objetivos: Caracterizar os médicos brasileiros que participaram do II Congresso Médico Latino-Americano e analisar as recomendações deste Congresso para a Enfermagem. Trata-se de um estudo histórico-social, embasado na análise documental. Resultados: A participação de médicos brasileiros neste evento foi grande. Em meio às discussões de ordem sanitária, figurou a questão da criação de escolas de enfermeiras, apresentada pelo médico Moisés Amaral. Diante das evidências da eficácia de se ter uma enfermeira instruída em escolas próprias, e de acordo com os preceitos da medicina moderna, foi aprovada a proposta para a criação de escolas de enfermagem. Apesar da posição estratégica que os representantes brasileiros nesse Congresso ocupavam no país, não encontramos evidências do empenho deles em trabalhar efetivamente para a criação de escolas de enfermeiras como uma estratégia para enfrentar os problemas sanitários.Estudio sobre las recomendaciones del II Congreso Médico Latino-Americano, en 1904, para la organización de la enfermería el Brasil. Objetivos: Caracterizar los médicos brasileños que participaran del II Congreso Médico Latino-Americano; y analizar las recomendaciones de este congreso para la enfermería. Estudio histórico-social basado en la análisis documental. Resultados: la participación brasileña en este acontecimiento foi grande. En medio a las discusiones sanitarias, figuró la cuestión de creación de escuelas de enfermeríapresentada por el médico Moisés Amaral. Frente a las evidencias de la eficacia de tener una enfermera instruida en escuelas apropiadas y de acuerdo con los preceptos de la medicina moderna fue aprobada la propuesta de creación de escuelas de enfermería. A pesar de la estratégica posición de los representantes brasileños en ese Congreso

  11. La frontera y la línea fronteriza: puntos de vista mexicanos y americanos de la frontera méxico–americana

    Directory of Open Access Journals (Sweden)

    Richard Griswold del Castillo

    1984-01-01

    Full Text Available A lo largo de los siglos, los mexicanos y estadounidenses han desarrollado conceptos diferentes con relación a su región fronteriza común. Muchas de las diferencias en perspectivas nacionales, son el resultado de sus culturas históricas y definiciones de interés propio. El propósito de este escrito es delinear en forma general el origen de perspectivas mexicanas y estadounidenses de la región geográfica que ha sido llamada la frontera México- Estados Unidos. Finalmente, el valor real de la historia consiste en que nos permite evaluar ideas y culturas en sus patrones que tienen raíces profundas en nuestra experiencia colectiva pasada. Históricamente, para mexicanos y americanos, la frontera ha sido un lugar de conflicto y mezcla cultural. No es ni ha sido nunca una zona homogénea, ni regional, ni internacionalmente. Es necesario que los que planean comprendan los puntos de convergencia y divergencia en actitudes mexicanas y anglo-mexicanas hacia la frontera común.

  12. Two new non-spiny Solanum (Solanaceae from the Gran Chaco Americano and a key for the herbaceous glandular-pubescent solanums from the region

    Directory of Open Access Journals (Sweden)

    Tiina Sarkinen

    2016-11-01

    Full Text Available The Gran Chaco Americano is a major savanna woodland system in South America that harbours great plant and animal diversity. Two new herbaceous species of the Morelloid clade of Solanum (largely corresponding to the traditional Solanum section Solanum are described here from the Bolivian Chaco. Both species are morphologically similar to a group of related species with glandular pubescence and enlarged, foliaceous calyces that includes S. atriplicifolium Gillies ex Nees, S. nitidibaccatum Bitter, S. physalifolium Rusby, S. sarrachoides Sendtn. and S. tweedianum Hook. Solanum woodii Särkinen & S.Knapp, sp. nov. is unusual in the Morelloid clade in having tapering anthers on short filaments, and is superficially similar to the unrelated S. anomalostemon S.Knapp & M.Nee from dry forests in Peru. Solanum michaelis Särkinen & S.Knapp, sp. nov. is distinct in its enlarged calyx with a short tube and long lobes that apparently reflex at fruit maturity. Both new taxa are illustrated, their conservation status assessed, and their distributions mapped. We also provide a key to the glandular-pubescent herbaceous Solanum species of the Chaco vegetation to aid in identification of these taxa.

  13. Comportamento eleitoral e tipologia da ação social weberiana: considerações sobre a tese do apartidarismo norte-americano

    Directory of Open Access Journals (Sweden)

    Éder Rodrigo Gimenes

    2014-07-01

    Full Text Available http://dx.doi.org/10.5007/1806-5023.2014v11n1p109   Entre as pesquisas que têm se dedicado à discussão sobre o partidarismo, muitas apontam o crescente distanciamento dos eleitores em relação aos partidos políticos. Nesse sentido, Dalton (2013 levanta a tese de que os Estados Unidos e as demais democracias avançadas estariam presenciando a expansão de um novo tipo de eleitor, apartidário. Diante dessas considerações, o presente artigo tem por objetivo evidenciar a influência do conceito de ação social de Weber sobre o modelo de tipificação dos perfis do eleitorado norte-americano. Em nosso entendimento, a proposta analítica de Dalton adota por princípio os modos de orientação das ações sociais de Weber.

  14. Técnica de Extração de Palinomorfos em Cálculos Dentários de Gonfotérios Sul-Americanos

    Directory of Open Access Journals (Sweden)

    Shana Yuri Misumi

    2016-01-01

    Full Text Available Foi desenvolvida uma técnica de laboratório para a extração de palinomorfos de cálculos dentários de indivíduos do gonfotério sul-americano Notiomastodon platensis. As amostras foram retiradas de espécimes molares provenientes de localidades do Nordeste e do Sul do Brasil. O procedimento constitui-se em etapas de maceração, adição de esporos exóticos de Lycopodium clavatum, reação com ácido clorídrico concentrado, lavagem com água destilada e tamisação por ultrassom. A preparação das lâminas com os palinomorfos obtidos fez uso de gelatina glicerinada. Foram recuperados grãos de pólen, esporos de pteridófitas e fragmentos de tecidos vegetais. Esta metodologia permite a extração e observação de outros microfósseis, como fitólitos, que também podem estar presentes em cálculos dentários de mamíferos herbívoros.

  15. Pasivos, exóticos, vencidos, víctimas. El indígena americano en la cultura oficial española del siglo XIX

    Directory of Open Access Journals (Sweden)

    Reyero, Carlos

    2004-12-01

    Full Text Available This article analizes the role that the american indian has represented in visual culture in Spain during the nineteenth century. The author takes into consideration the history painting and the monuments erected in this period. It is possible to recognise four main issues: 1 The passive, with a double slope political and religious, that conduce to exhibit a natural superiority of spanish people; 2 The resigned and exotic personality, connected to a picturesque vision far from any conflict; 3 The powerful enemy, defeated in spite of his courage; and 4 The considerable nobility, connected to the character as much as the rising of liberal spirit.

    Este artículo analiza el papel que ha representado el indio americano en la cultura visual española durante el siglo XIX. El autor tiene en cuenta la pintura de historia y los monumentos erigidos en este periodo. Es posible reconocer cuatro grandes orientaciones: 1 El pasivo, con una doble vertiente política y religiosa, que conduce a mostrar la natural superioridad del español; 2 El exotismo sumiso, relacionado con una visión pintoresca alejada de cualquier conflicto; 3 El enemigo poderoso, al que sin embargo se derrota; y 4 La caballerosidad respetuosa, relacionada tanto con el carácter como con el afianzamiento del espíritu liberal.

  16. "The Americano Dream" ou "The American Dream": o debate contemporâneo sobre a imigração de mexicanos para os Estados Unidos

    Directory of Open Access Journals (Sweden)

    Celso Antônio Coelho Vaz

    2014-12-01

    Full Text Available Neste artigo resenho o debate acadêmico suscitado nos Estados Unidos por causa dos argumentos de Huntington sobre a imigração hispânica, sobretudo a de mexicanos, para este país. Em suas obras "The Hispanic challenge" e Who are we?, Huntington argumenta que, por razões demográficas e políticas, os imigrantes mexicanos não se assimilariam ao core culture norte-americano fundado nos valores anglo-protestantes, o que dividiria os EUA em dois povos, duas culturas e duas linguagens. As pesquisas empíricas aqui resenhadas que testaram os argumentos empíricos de Huntington os negaram e os críticos teóricos destes concebem que eles são doutrinários porque expressam uma ideologia settler, bem como manifestam a teoria do conflito do pluralismo cultural e a do colonianismo interno.

  17. Nodulação em cultivares de feijão dos conjuntos gênicos andino e meso-americano

    Directory of Open Access Journals (Sweden)

    Franco Marília Caixeta

    2002-01-01

    Full Text Available O objetivo deste trabalho foi determinar a capacidade de nodulação e a especificidade de feijão (Phaseolus vulgaris L. dos conjuntos gênicos andino e meso-americano submetidos a inóculo de Rhizobium. O experimento foi estabelecido em parcelas subdivididas em blocos ao acaso, com quatorze cultivares de feijão e três estirpes de Rhizobium (R. etli KIM 5, R. etli CIAT 632 e R. tropici CIAT 899. Somente as cultivares andinas WAF 15, WAF 7, Mineiro Precoce, WAF 6 e Antioquia 8 apresentaram especificidade na nodulação. Em relação à massa seca dos nódulos, houve diferenças significativas dos tratamentos de inoculação nas cultivares andinas WAF 15, WAF 7, WAF 6 e Diacol Andino, e na cultivar meso-americana Ouro Negro. Nenhuma das cultivares restringiu a nodulação, embora tenham sido verificadas diferenças de até 53 e 103 vezes no número e massa de nódulos por planta, respectivamente. Considerando todas as cultivares e estirpes de rizóbio, WAF 15 foi a cultivar com melhor desempenho em número e massa nodular. WAF 6 foi a cultivar de pior desempenho, chegando quase ao nível de restrição da nodulação com a estirpe R. etli CIAT 632.

  18. O pensamento conservador ibero-americano na era das independências (1808-1850 Conservative ibero-american thought in the age of independences (1808-1850

    Directory of Open Access Journals (Sweden)

    Christian Edward Cyril Lynch

    2008-01-01

    Full Text Available A intenção do artigo é fornecer um panorama ideológico da América Ibérica da época das independências, especialmente no que diz respeito às ideologias de direita. Busca-se, assim, definir o que seja conservadorismo, distinguindo-o do puro reacionarismo para, em seguida, comparar a orientação de José Bonifácio e de Marquês de Caravelas, no Brasil, com aquela desenvolvida por Bolívar, tendo em vista os dilemas do conservadorismo ibero-americano. A comparação esclarece as diferentes trajetórias da antiga América portuguesa e da antiga América hispânica, no decorrer do século XIX.This article intends to study the right-wing ideologies of Latin America during the first half of the nineteenth century. At first, it tries to define what conservatism is and to point out its differences from the rhetoric of reaction; later, the thought of José Bonifacio and the Marquis of Caravelas is compared to that of Simon Bolivar, underlying the dilemmas of Latin American conservatism. The comparison enlightens the different ways followed by the ancient Portuguese and Hispanic America after political independence.

  19. A switch I mutant of Cdc42 exhibits decreased conformational freedom

    Science.gov (United States)

    Chandrashekar, Reena; Salem, Omar; Krizova, Hana; McFeeters, Robert; Adams, Paul D.

    2011-01-01

    Cdc42 is a Ras-related small G-protein, and functions as a molecular switch in signal transduction pathways linked with cell growth and differentiation. It is controlled by cycling between GTP-bound (active) and GDP-bound (inactive) forms. Nucleotide binding and hydrolysis are modulated by interactions with effectors and/or regulatory proteins. These interactions are centralized in two relatively flexible “Switch” regions as characterized by internal dynamics on multiple timescales (Loh et al., (2001) Biochemistry 40, 4590–4600), and this flexibility may be essential for protein interactions. In the Switch I region, Thr35 seems critical for function, as it is completely invariant in Ras-related proteins. To investigate the importance of conformational flexibility in Switch I of Cdc42, we mutated threonine to alanine, determined the solution structure and characterized the backbone dynamics of the single-point mutant protein, Cdc42(T35A). Backbone dynamics data suggests that the mutation changes the timescale of the internal motions of several residues, with several resonances appearing not discernable in Cdc42 wild type (Adams and Oswald (2007) Biomolecular NMR Assignments 1, 225–227). The mutation does not appear to affect the thermal stability of Cdc42, and chymotrypsin digestion data further suggests that changes in conformational flexibility in Switch I slow proteolytic cleavage relative to wild type. In-vitro binding assays show reduced binding of Cdc42(T35A), relative to wild type, to a GTPase binding protein that inhibits GTP hydrolysis in Cdc42. These results suggest that the mutation of T35 leads to the loss of conformational freedom in Switch I that could affect effector/regulatory protein interactions. PMID:21667996

  20. Reflexiones sobre los sistemas silvopastoriles

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    R. O Russo

    Full Text Available El objetivo de este análisis es reflexionar acerca de modelos integrados de producción ganadera familiar y extensiva, más estables y sustentables, en los que se integre el componente leñoso, que contribuye a la reducción de gases con efecto invernadero y favorece la mitigación del cambio climático. Para ello se hizo una revisión de conceptos referentes a la integración de la actividad forestal en la ganadería, como alternativa viable de sistema de producción. Se parte del criterio de que los sistemas silvopastoriles (SSP, dentro de los agroforestales, son agroecosistemas en los que se asocia un componente arbóreo con uno herbáceo (pasturas naturales o mejoradas y otro pecuario (ganado en un mismo sitio, donde existen interacciones biológicas entre estos y se maximiza el uso de la tierra. También se describe cómo se agrupan los SSP; sus oportunidades desde los puntos de vista económico, productivo, social y ambiental; y los efectos de la interacción entre sus componentes. Este análisis permite plantear que los SSP son producto de la relación entre la biología, la sociedad y la cultura, y en ellos existe una enorme diversidad; asimismo, permiten la reconversión de la ganadería extensiva de muy baja productividad en sistemas más productivos y sostenibles en el tiempo, así como la rehabilitación de las áreas degradadas por ese tipo de ganadería, por la deforestación y por el agotamiento de los suelos.

  1. Sistema prisional e direitos humanos

    OpenAIRE

    Mendes, André Pacheco Teixeira

    2015-01-01

    O volume 4 da Série Clínicas dos Cadernos FGV DIREITO RIO traz quatro trabalhos originais relacionados ao tema do Sistema Prisional e Direitos Humanos. A obra contempla pareceres jurídicos sobre violações aos direitos dos presos nas unidades prisionais do estado do Rio de Janeiro, tendo por objeto: (i) emprego ilegal de laxante em custodiados e visitantes suspeitos de carregarem drogas; (ii) falta de água própria para o consumo humano; (iii) restrição ao banho de sol; e (iv) condições inadequ...

  2. Neurocitoma no sistema nervoso central

    Directory of Open Access Journals (Sweden)

    José Torquato Severo

    1973-03-01

    Full Text Available É relatado o caso de uma paciente com 6 anos de idade, hospitalizada com síndrome de hipertensão intracraniana. Após o exame neuro-radiológico que evidenciou processo expansivo frontal direito, a paciente foi submetida à cirurgia, durante a qual ocorreu o óbito. O exame histo-patológico do material retirado durante o ato operatório, permitiu o diagnóstico de neurocitoma, tumor raro no sistema nervoso central.

  3. Sistemas de control en estructuras

    OpenAIRE

    José Luis Ahumada Villafañe

    2010-01-01

    En el diseño estructural, la capacidad de disipación de energía en elementos que conforman los sistemas resistentes a fuerzas dinámicas, como las impartidas por la actividad sísmica y eólica, es suministrada por un minucioso detallado del acero de refuerzo y dimensiones en las secciones transversales de los elementos. Esta práctica es realizada bajo muchas suposiciones que en algunas ocasiones no corresponden a la realidad, y como es de esperarse, producen resultados inexactos y un desconocim...

  4. Mechanisms of Cdc42-mediated rat MSC differentiation on micro/nano-textured topography.

    Science.gov (United States)

    Li, Guangwen; Song, Yanyan; Shi, Mengqi; Du, Yuanhong; Wang, Wei; Zhang, Yumei

    2017-02-01

    Micro/nano-textured titanium surface topography promotes osteoblast differentiation and the Wnt/β-catenin signaling pathway. However, the response of rat bone mesenchymal stem cells (MSCs) to micro/nano-textured topography, and the underlying mechanisms of its effects, are not well understood. We hypothesized that cell division cycle 42 protein (Cdc42), a key member of the Rho GTPases family, may regulate rat MSCs morphology and osteogenic differentiation by micro/nano-textured topography, and that crosstalk between Cdc42 and Wnt/β-catenin is the underlying mechanism. To confirm the hypothesis, we first tested rat MSCs' morphology, cytoskeleton, and osteogenic differentiation on micro/nano-textured topography. We then examined the cells' Wnt pathway and Cdc42 signaling activity. The results show that micro/nano-textured topography enhances MSCs' osteogenic differentiation. In addition, the cells' morphology and cytoskeletal reorganization were dramatically different on smooth surfaces and micropitted/nanotubular topography. Ligands of the canonical Wnt pathway, as well as accumulation of β-catenin in the nucleus, were up-regulated by micro/nano-textured topography. Cdc42 protein expression was markedly increased under these conditions; conversely, Cdc42 silencing significantly depressed the enhancement of MSCs osteogenic differentiation by micro/nano-textured topography. Moreover, Cdc42si attenuated p-GSK3β activation and resulted in β-catenin cytoplasmic degradation on the micro/nano-textured topography. Our results indicate that Cdc42 is a key modulator of rat MSCs morphology and cytoskeletal reorganization, and that crosstalk between Cdc42 and Wnt/β-catenin signaling though GSK3β regulates MSCs osteogenic differentiation by implant topographical cues. Topographical modification at micro- and nanoscale is widely applied to enhance the tissue integration properties of biomaterials. However, the response of bone mesenchymal stem cells (MSCs) to the micro

  5. CDC-reported assisted reproductive technology live-birth rates may mislead the public.

    Science.gov (United States)

    Kushnir, Vitaly A; Choi, Jennifer; Darmon, Sarah K; Albertini, David F; Barad, David H; Gleicher, Norbert

    2017-08-01

    The Centre for Disease Control and Prevention (CDC) publicly reports assisted reproductive technology live-birth rates (LBR) for each US fertility clinic under legal mandate. The 2014 CDC report excluded 35,406 of 184,527 (19.2%) autologous assisted reproductive technology cycles that involved embryo or oocyte banking from LBR calculations. This study calculated 2014 total clinic LBR for all patients utilizing autologous oocytes two ways: including all initiated assisted reproductive technology cycles or excluding banking cycles, as done by the CDC. The main limitation of this analysis is the CDC report did not differentiate between cycles involving long-term banking of embryos or oocytes for fertility preservation from cycles involving short-term embryo banking. Twenty-seven of 458 (6%) clinics reported over 40% of autologous cycles involved banking, collectively performing 12% of all US assisted reproductive technology cycles. LBR in these outlier clinics calculated by the CDC method, was higher than the other 94% of clinics (33.1% versus 31.1%). However, recalculated LBR including banking cycles in the outlier clinics was lower than the other 94% of clinics (15.5% versus 26.6%). LBR calculated by the two methods increasingly diverged based on proportion of banking cycles performed by each clinic reaching 4.5-fold, thereby, potentially misleading the public. Copyright © 2017 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

  6. Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1

    DEFF Research Database (Denmark)

    Du, Dan; Pedersen, Esben; Wang, Zhipeng

    2008-01-01

    Cell-cell contacts are crucial for the integrity of all tissues. Contrasting reports have been published about the role of Cdc42 in epithelial cell-cell contacts in vitro. In keratinocytes, it was suggested that Rac1 and not Cdc42 is crucial for the formation of mature epithelial junctions, based....... Inhibition of aPKCzeta by the inhibitor Gö6983 reproduced the phenotype, suggesting that decreased activation of aPKCzeta was sufficient to explain the defective junctional maturation. In the absence of Cdc42, Rac1 activation was strongly decreased, indicating that Cdc42 is upstream of Rac1 activation...

  7. The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse

    DEFF Research Database (Denmark)

    Vasyutina, Elena; Martarelli, Benedetta; Brakebusch, Cord

    2009-01-01

    Rac1 and Cdc42 are small G-proteins that regulate actin dynamics and affect plasma membrane protrusion and vesicle traffic. We used conditional mutagenesis in mice to demonstrate that Rac1 and Cdc42 are essential for myoblast fusion in vivo and in vitro. The deficit in fusion of Rac1 or Cdc42...... mutant myoblasts correlates with a deficit in the recruitment of actin fibers and vinculin to myoblast contact sites. Comparison of the changes observed in mutant myogenic cells indicates that Rac1 and Cdc42 function in a nonredundant and not completely overlapping manner during the fusion process. Our...

  8. Institucionalização do Sistema Interamericano de Combate às Drogas: iniciativas e progressos

    Directory of Open Access Journals (Sweden)

    Matheus Bernardes Rachadel

    2014-11-01

    Full Text Available http://dx.doi.org/10.5007/1806-5023.2013v10n2p25 O presente artigo analisa o tratamento do fenômeno das drogas na América Latina, discutindo os avanços na criação de um sistema continental de combate às drogas, representado pela Comissão Interamericana para o Controle do Abuso de Drogas (CICAD. A primeira parte do trabalho traça uma retrospectiva da criação da CICAD como iniciativa no surgimento de um arcabouço institucional e legal de tratamento do tema das drogas nas Américas. A segunda parte aborda o processo de consolidação da CICAD como instituição central nesse regime, focada em ultrapassar os métodos tradicionais de enfrentamento do fenômeno. Conclui-se que, ao longo da segunda metade do século XX, os países latino-americanos reagiram diante do fenômeno das drogas e que a criação de um regime interamericano para o tema foi fundamental para o fomento da cooperação regional nessa problemática.

  9. Fortalecimiento del sistema judicial del Uruguay por la capacitación del sector humano; de la organización e integración de las escuelas judiciales americanos

    Directory of Open Access Journals (Sweden)

    Mariella Leles Da Silva Orcoyen

    2015-10-01

    Full Text Available Contenido: Introducción. Poder judicial. Ministerio Público y Fiscal. Estatuto de los jueces. Reforma procesal.  El fortalecimiento de la justicia por la reforma procesal. Centro de Estudios Judiciales del Uruguay. Capacitación.Capacitación para el ingreso a la magistratura. Resultados obtenidos. Estadísticas. Perspectivas futuras. Rol del Centro de Estudios Judiciales del Uruguay en la carrera judicial. Autoridades del Centro de Estudios Judiciales del Uruguay. Federación Americana de Escuelas de Formación y Perfeccionamiento de Magistrados y Centros de Estudios Judiciales

  10. Analysis of the influence of the expansion of the South American electric system in emissions of greenhouse gases; Analise da influencia da expansao do sistema eletrico Sul-Americano nas emissoes de gases de efeito estufa

    Energy Technology Data Exchange (ETDEWEB)

    Castagna, Annemarlen Gehrke [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Blesl, Markus [Institute of Economics and the Rational Use of Energie (IER), Stuttgart (Germany)

    2010-07-01

    South America combines economic and population growth with a consequent rapid increase in electricity demand. This can only be covered by building new power plants, use of the remaining renewable potential and expansion of transmission lines. The expansion of supply in all regions, with reliable generation and transmission systems is the greatest challenge for the continent in order to reduce social differences and not to curb economic development. To support the energy planning the application of system models represents useful method. This paper intends to analyze the expansion effect of power plant parks in regard of greenhouse gases emissions using a regionalized model system 'TIMES (The Integrated Markal - EFOM System)'. The model includes 10 South American countries (Argentina, Brazil, Bolivia, Chile, Colombia, Ecuador, Paraguay, Peru, Uruguay and Venezuela) with their respective power parks and transmission lines, demand divided in sectors, potential use of renewable energy sources, gas pipelines and possibilities of new interconnections within and between countries. As results are obtained the future installed capacity and generation according the energy use, greenhouse gases emissions, as well as the investments needed to expand the electric system in different scenarios. (author)

  11. Distrito federal electoral No.34 del estado de California: un estudio de caso sobre la inclusión y representación de ciudadanos americanos de origen mexicano en el sistema político estadounidense

    OpenAIRE

    Cebreros Félix, Erika Eloisa

    2004-01-01

    A partir del censo poblacional de Estados Unidos de América de 2000, se determinó que los hispanos conformarían el grupo minoritario más grande del país, por lo que se les empezó a visualizar en términos políticos y específicamente electorales. Sin embargo, la historia y evolución de la inclusión y representación de los diferentes subgrupos que conforman dicha minoría ha sido poco desarrollada. El concepto de representación es uno de los aspectos fundamentales en el estud...

  12. Structure and function of the AAA+ ATPase p97/Cdc48p.

    Science.gov (United States)

    Xia, Di; Tang, Wai Kwan; Ye, Yihong

    2016-05-25

    p97 (also known as valosin-containing protein (VCP) in mammals or Cdc48p in Saccharomyces cerevisiae) is an evolutionarily conserved ATPase present in all eukaryotes and archaebacteria. In conjunction with a collection of cofactors and adaptors, p97/Cdc48p performs an array of biological functions mostly through modulating the stability of 'client' proteins. Using energy from ATP hydrolysis, p97/Cdc48p segregates these molecules from immobile cellular structures such as protein assemblies, membrane organelles, and chromatin. Consequently, the released polypeptides can be efficiently degraded by the ubiquitin proteasome system or recycled. This review summarizes our current understanding of the structure and function of this essential cellular chaperoning system. Published by Elsevier B.V.

  13. MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process.

    Science.gov (United States)

    Dong, Ying; Jin, Xintian; Sun, Zhiqiang; Zhao, Yueming; Song, Xianjing

    2017-03-01

    In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3'UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment.

  14. CDC Activities for Improving Implementation of Human Papillomavirus Vaccination, Cervical Cancer Screening, and Surveillance Worldwide.

    Science.gov (United States)

    Senkomago, Virginia; Duran, Denise; Loharikar, Anagha; Hyde, Terri B; Markowitz, Lauri E; Unger, Elizabeth R; Saraiya, Mona

    2017-12-01

    Cervical cancer incidence and mortality rates are high, particularly in developing countries. Most cervical cancers can be prevented by human papillomavirus (HPV) vaccination, screening, and timely treatment. The US Centers for Disease Control and Prevention (CDC) provides global technical assistance for implementation and evaluation of HPV vaccination pilot projects and programs and laboratory-related HPV activities to assess HPV vaccines. CDC collaborates with global partners to develop global cervical cancer screening recommendations and manuals, implement screening, create standardized evaluation tools, and provide expertise to monitor outcomes. CDC also trains epidemiologists in cancer prevention through its Field Epidemiology Training Program and is working to improve cancer surveillance by supporting efforts of the World Health Organization in developing cancer registry hubs and assisting countries in estimating costs for developing population-based cancer registries. These activities contribute to the Global Health Security Agenda action packages to improve immunization, surveillance, and the public health workforce globally.

  15. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1.

    Science.gov (United States)

    Lee, Seung Baek; Kim, Jung Jin; Nam, Hyun-Ja; Gao, Bowen; Yin, Ping; Qin, Bo; Yi, Sang-Yeop; Ham, Hyoungjun; Evans, Debra; Kim, Sun-Hyun; Zhang, Jun; Deng, Min; Liu, Tongzheng; Zhang, Haoxing; Billadeau, Daniel D; Wang, Liewei; Giaime, Emilie; Shen, Jie; Pang, Yuan-Ping; Jen, Jin; van Deursen, Jan M; Lou, Zhenkun

    2015-10-01

    Mutations in the E3 ubiquitin ligase Parkin have been linked to familial Parkinson's disease. Parkin has also been implicated in mitosis through mechanisms that are unclear. Here we show that Parkin interacts with anaphase promoting complex/cyclosome (APC/C) coactivators Cdc20 and Cdh1 to mediate the degradation of several key mitotic regulators independent of APC/C. We demonstrate that ordered progression through mitosis is orchestrated by two distinct E3 ligases through the shared use of Cdc20 and Cdh1. Furthermore, Parkin is phosphorylated and activated by polo-like kinase 1 (Plk1) during mitosis. Parkin deficiency results in overexpression of its substrates, mitotic defects, genomic instability, and tumorigenesis. These results suggest that the Parkin-Cdc20/Cdh1 complex is an important regulator of mitosis. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. Should all patients with hyperparathyroidism be screened for a CDC73 mutation?

    Directory of Open Access Journals (Sweden)

    Caroline Bachmeier

    2018-03-01

    Full Text Available Primary hyperparathyroidism (PH is a common endocrine abnormality and may occur as part of a genetic syndrome. Inactivating mutations of the tumour suppressor gene CDC73 have been identified as accounting for a large percentage of hyperparathyroidism-jaw tumour syndrome (HPT-JT cases and to a lesser degree account for familial isolated hyperparathyroidism (FIHP cases. Reports of CDC73 whole gene deletions are exceedingly rare. We report the case of a 39 year-old woman with PH secondary to a parathyroid adenoma associated with a large chromosomal deletion (2.5 Mb encompassing the entire CDC73 gene detected years after parathyroidectomy. This case highlights the necessity to screen young patients with hyperparathyroidism for an underlying genetic aetiology. It also demonstrates that molecular testing for this disorder should contain techniques that can detect large deletions.

  17. [CDC73 mutations in young patients with primary hyperparathyroidism: A description of two clinical cases].

    Science.gov (United States)

    Mamedova, E O; Mokrysheva, N G; Pigarova, E A; Przhiyalkovskaya, E G; Voronkova, I A; Vasilyev, E V; Petrov, V M; Gorbunova, V A; Rozhinskaya, L Ya; Belaya, Zh E; Tyulpakov, A N

    The article describes two clinical cases of severe primary hyperparathyroidism (PHPT) caused by parathyroid carcinoma in young female patients who underwent molecular genetic testing to rule out the hereditary forms of PHPT. In both patients, heterozygous germline nonsense mutations of tumor suppressor gene CDC73 encoding parafibromin (p.R91X and p.Q166X) were identified using next-generation sequencing with Ion Torrent Personal Genome Machine (Thermo Fisher Scientific - Life Technologies, USA). It is the first description of CDC73 mutations in Russia, one of the mutations is described for the first time in the world. Identification of germline mutations in the CDC73 gene in patients with PHPT necessitates regular lifelong screening for other manifestations of hyperparathyroidism-jaw tumor syndrome (HPT-JT), PHPT recurrence due to parathyroid carcinoma as well, and identification of mutation carriers among first-degree relatives.

  18. Cdc2/cyclin B1 regulates centrosomal Nlp proteolysis and subcellular localization.

    Science.gov (United States)

    Zhao, Xuelian; Jin, Shunqian; Song, Yongmei; Zhan, Qimin

    2010-11-01

    The formation of proper mitotic spindles is required for appropriate chromosome segregation during cell division. Aberrant spindle formation often causes aneuploidy and results in tumorigenesis. However, the underlying mechanism of regulating spindle formation and chromosome separation remains to be further defined. Centrosomal Nlp (ninein-like protein) is a recently characterized BRCA1-regulated centrosomal protein and plays an important role in centrosome maturation and spindle formation. In this study, we show that Nlp can be phosphorylated by cell cycle protein kinase Cdc2/cyclin B1. The phosphorylation sites of Nlp are mapped at Ser185 and Ser589. Interestingly, the Cdc2/cyclin B1 phosphorylation site Ser185 of Nlp is required for its recognition by PLK1, which enable Nlp depart from centrosomes to allow the establishment of a mitotic scaffold at the onset of mitosis . PLK1 fails to dissociate the Nlp mutant lacking Ser185 from centrosome, suggesting that Cdc2/cyclin B1 might serve as a primary kinase of PLK1 in regulating Nlp subcellular localization. However, the phosphorylation at the site Ser589 by Cdc2/cyclin B1 plays an important role in Nlp protein stability probably due to its effect on protein degradation. Furthermore, we show that deregulated expression or subcellular localization of Nlp lead to multinuclei in cells, indicating that scheduled levels of Nlp and proper subcellular localization of Nlp are critical for successful completion of normal cell mitosis, These findings demonstrate that Cdc2/cyclin B1 is a key regulator in maintaining appropriate degradation and subcellular localization of Nlp, providing novel insights into understanding on the role of Cdc2/cyclin B1 in mitotic progression.

  19. Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Zoltán Kevei

    Full Text Available The CDC20 and Cdh1/CCS52 proteins are substrate determinants and activators of the Anaphase Promoting Complex/Cyclosome (APC/C E3 ubiquitin ligase and as such they control the mitotic cell cycle by targeting the degradation of various cell cycle regulators. In yeasts and animals the main CDC20 function is the destruction of securin and mitotic cyclins. Plants have multiple CDC20 gene copies whose functions have not been explored yet. In Arabidopsis thaliana there are five CDC20 isoforms and here we aimed at defining their contribution to cell cycle regulation, substrate selectivity and plant development.Studying the gene structure and phylogeny of plant CDC20s, the expression of the five AtCDC20 gene copies and their interactions with the APC/C subunit APC10, the CCS52 proteins, components of the mitotic checkpoint complex (MCC and mitotic cyclin substrates, conserved CDC20 functions could be assigned for AtCDC20.1 and AtCDC20.2. The other three intron-less genes were silent and specific for Arabidopsis. We show that AtCDC20.1 and AtCDC20.2 are components of the MCC and interact with mitotic cyclins with unexpected specificity. AtCDC20.1 and AtCDC20.2 are expressed in meristems, organ primordia and AtCDC20.1 also in pollen grains and developing seeds. Knocking down both genes simultaneously by RNAi resulted in severe delay in plant development and male sterility. In these lines, the meristem size was reduced while the cell size and ploidy levels were unaffected indicating that the lower cell number and likely slowdown of the cell cycle are the cause of reduced plant growth.The intron-containing CDC20 gene copies provide conserved and redundant functions for cell cycle progression in plants and are required for meristem maintenance, plant growth and male gametophyte formation. The Arabidopsis-specific intron-less genes are possibly "retrogenes" and have hitherto undefined functions or are pseudogenes.

  20. Depois das reformas: os regimes de proteção social latino-americanos na década de 2000

    Directory of Open Access Journals (Sweden)

    Rodrigo Cantu

    2015-12-01

    Full Text Available Resumo O artigo caracteriza os regimes de política social na América Latina durante a década de 2000, um contexto que vem sendo designado por uma penetração menos acentuada de políticas neoliberais. Usamos o método de Análise de Componentes Principais (ACP para analisar dados sobre o papel do Estado, do mercado e das famílias na proteção contra riscos sociais, recolhidos em fontes como CEPAL, FMI, Banco Mundial, OMS, entre outras. Comparando os dados para as décadas de 1990 e de 2000, identificamos certa estabilização das reformas neoliberais. Comparando ainda os resultados da ACP com a literatura, sobretudo com a literatura que se ocupa das décadas de 1980 e 1990, argumentamos que, mesmo atenuadas, as reformas deixaram o cenário da política social mais complexo na América Latina na década de 2000. Uma primeira dimensão, já amplamente discutida na literatura, foi encontrada na ACP, definida pelo grau em que o Estado inclui a população em suas políticas sociais. Ademais, uma segunda dimensão relevante foi revelada pela análise, captando em que profundidade o mercado se instalou como princípio da política social. Discutimos a hipótese segundo a qual essa dimensão se tornou expressiva após as reformas das décadas de 1980 e 1990. Junto com o argumento de que a literatura precisa ser atualizada com base nesse quadro mais complexo, destacamos a diversidade dos regimes de política social latino-americanos tanto internamente quanto em comparação com outras regiões do mundo.

  1. Las maestras y el ensayo como escritura cívica femenina en Repertorio Americano / Women Teachers, Essays and Civic Writing in “Repertorio Americano”

    Directory of Open Access Journals (Sweden)

    Marybel Soto Ramírez

    2011-11-01

    Full Text Available Recibido 17 de mayo de 2011 • Aceptado 26 de agosto de 2011 • Corregido 9 de octubre de 2011 Resumen. La escritura femenina, como todas las formas de producción simbólica desde las mujeres, ha existido y se ha desarrollado a pesar del mandato patriarcal de obedecer y callar que pesa sobre el género femenino. No es objetivo en esta reflexión, ahondar en las acciones y las obras de las mujeres transgresoras quienes, a lo largo de la historia y a pesar de, han ejercido esa forma de hacerse presente y afirmarse ante los otros que es la escritura. Mi propósito es identificar a las mujeres, quienes desde el oficio de maestras ejercitaron su escritura, combinaron su rol docente con el de escritoras y en la elaboración de sus discursos cívicos cuestionaron, precisamente, las concepciones de patriotismo y sentimiento cívico dentro del mismo proceso de construcción de una ciudadanía de la cual ellas no disfrutaban. Abstract. Women writing, as any symbolic form of production, has existed and developed despite of the patriarchal imperative commanding women to obey and to remain silent. It is not the objective of this essay to deepen on the deeds and doings of those women who, throughout history and despite patriarchal mandates, have exerted their writing as a transgression as a way to show and to affirm themselves before others. Moreover, my purpose is to identify women in Repertorio Americano (1919-1958 who as teachers and writers elaborated civic discourses and questioned the very meaning of patriotism and civic feelings in the constructions of a citizenship they were not fully and formally entitled to.

  2. Atividade anti-helmíntica de plantas nativas do continente americano: uma revisão Anthelmintic activity of native American plants: a review

    Directory of Open Access Journals (Sweden)

    R.G. Sousa

    2013-01-01

    Full Text Available O presente trabalho faz uma revisão de plantas nativas do continente americano com atividade anti-helmíntica com a finalidade de subsidiar pesquisas e o desenvolvimento de novos medicamentos na área de produtos naturais. Na literatura, foram citadas 22 famílias de plantas, tais como: Moraceae, Asteraceae e Cucurbitaceae, e estudadas cerca de 35 espécies, como a Ficus sp, Cucurbita máxima, e Ruta graveolens, usadas por sua alegada atividade anti-helmíntica. Destas espécies, 26 foram ativas contra alguns helmintos: Strongyloides stercolaris, Ancylostomidae, Trichostrongylus sp, Capillaria sp, Ascaris lumbricoides, Taenia saginata, Haemonchus contortus, e Hymenolepsis diminuta. Foi verificado maior número de estudos com parasitas de animais, possivelmente devido ao desenvolvimento de resistência dos helmintos aos tratamentos convencionais disponíveis.This paper is a review of native American plants showing anthelmintic activity in order to give support to research and development of new drugs in the field of natural products. In the literature, 22 plant families were cited, such as: Moraceae, Asteraceae and Cucurbitaceae, and around 35 species were studied, including Ficus sp, Cucurbita maxima and Ruta graveolens, used for their putative anthelmintic activity. From these species, 26 were active against some helminthes: Strongyloides stercolaris, Ancylostomidae, Trichostrongylus sp, Capillaria sp, Ascaris lumbricoides, Taenia saginata, Haemonchus contortus and Hymenolepsis diminuta. Data indicated a larger number of studies with animal parasites, probably due to the resistance of these helminthes to the conventional treatments available.

  3. Experience in programming Assembly language of CDC CYBER 170/750 computer

    International Nuclear Information System (INIS)

    Caldeira, A.D.

    1987-10-01

    Aiming to optimize processing time of BCG computer code in the CDC CYBER 170/750 computer, the FORTRAN-V language of INTERP subroutine was converted to Assembly language. The BCG code was developed for solving neutron transport equation by iterative method, and the INTERP subroutine is innermost loop of the code carrying out 5 interpolation types. The central processor unit Assembly language of the CDC CYBER 170/750 computer and its application in implementing the interpolation subroutine of BCG code are described. (M.C.K.)

  4. Cdc42 controls progenitor cell differentiation and beta-catenin turnover in skin

    DEFF Research Database (Denmark)

    Wu, Xunwei; Quondamatteo, Fabio; Lefever, Tine

    2006-01-01

    Differentiation of skin stem cells into hair follicles (HFs) requires the inhibition of beta-catenin degradation, which is controlled by a complex containing axin and the protein kinase GSK3beta. Using conditional gene targeting in mice, we show now that the small GTPase Cdc42 is crucial...... for differentiation of skin progenitor cells into HF lineage and that it regulates the turnover of beta-catenin. In the absence of Cdc42, degradation of beta-catenin was increased corresponding to a decreased phosphorylation of GSK3beta at Ser 9 and an increased phosphorylation of axin, which is known to be required...... progenitor cells in vivo....

  5. El sistema reticulo-endotelial

    Directory of Open Access Journals (Sweden)

    Carlos M. Pava

    1936-05-01

    Full Text Available El lector aficionado a los estudios histológicos que hace unos veinte años hubiese hojeado algún tratado nuevo sobre la materia, habría encontrado el término de "Sistema retículo-endotelial", sin duda extraño y desconocido para él, porque solamente entonces empezó a figurar no sólo como tal, sino principalmente como la expresión de una nueva agrupación de tejidos cuya morfología era semejante en líneas generales, y cuyo funcionamiento, a pesar de estar diseminado por todo el organismo, es de una precisión e igualdad admirables.

  6. Immanuel Wallerstein e o moderno sistema mundial

    Directory of Open Access Journals (Sweden)

    José Jobson de Andrade Arruda

    1983-12-01

    Full Text Available (primeiro parágrafo do texto Immanuel Wallerstein é diretor do Centro Fernand Braudel para o Estudo de Economias, Sistemas Históricos e Civilizacionais (Binghamton, N.Y. e editor da revista trimestral Review. Seu projeto mais amplo é a busca dos elementos determinantes do sistema mundial moderno. Para tanto, dividiu o seu trabalho em quatro partes principais, às quais con-sidera as quatro épocas fundamentais apresentadas pelo dito sistema até hoje. O primeiro volume, objeto desta resenha crítica, cobre o período de 1450 a 1640, correspondendo ao estudo das origens e condições primitivas do sistema mundial até então exclusivamente um sistema europeu. O segundo volume, já publicado, ocupar-se-ia da consolidação do sis-tema entre 1640 e 1815. O terceiro volume, referente ao período de 1815 a 1917, estará centrado na recriação do sistema decorrente das vertiginosas transformações resultantes do dinamismo tecnológico e do avanço do industrialismo no século XIX. O quarto volume cuidará da consolidação da economia — mundo capitalista, a partir de 1917 até a atualidade, e das tensões "revolucionárias" particulares que esta conso lidação tem provocado.

  7. 13 CFR 120.852 - Restrictions regarding CDC participation in the Small Business Investment Company (SBIC) program...

    Science.gov (United States)

    2010-01-01

    .... A CDC must not invest in or be an Affiliate of a Lender participating in the 7(a) loan program... of November 6, 2003 may remain Affiliates. (b) SBIC program. A CDC must not directly or indirectly... participation in the Small Business Investment Company (SBIC) program and the 7(a) loan program. 120.852 Section...

  8. 77 FR 76046 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Health...

    Science.gov (United States)

    2012-12-26

    ... HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD... subcommittee will provide advice to the CDC Director through the ACD on strategic and other broad issues facing... More Information: Leandris Liburd, Ph.D., M.P.H., M.A., Designated Federal Officer, HDS, ACD, CDC, 1600...

  9. 75 FR 61505 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Health...

    Science.gov (United States)

    2010-10-05

    ... HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD... to the ACD on strategic and other broad issues facing CDC. Matters To Be Discussed: Policy brief on..., ACD, CDC, 1600 Clifton Road, NE., M/S E-67, Atlanta, Georgia 30333. Telephone (404) 498-2310, E- mail...

  10. Make a Difference at Your School! CDC Resources Can Help You Implement Strategies to Prevent Obesity Among Children and Adolescents

    Science.gov (United States)

    Centers for Disease Control and Prevention, 2008

    2008-01-01

    The Centers for Disease Control and Prevention (CDC) reviews scientific evidence to determine which school-based policies and practices are most likely to improve key health behaviors among young people, including physical activity and healthy eating. In this document, the CDC identifies ten strategies to help schools prevent obesity by promoting…

  11. Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1

    DEFF Research Database (Denmark)

    Petersen, B O; Wagener, C; Marinoni, F

    2000-01-01

    CDC6 is conserved during evolution and is essential and limiting for the initiation of eukaryotic DNA replication. Human CDC6 activity is regulated by periodic transcription and CDK-regulated subcellular localization. Here, we show that, in addition to being absent from nonproliferating cells, CD...

  12. Hepatocyte-specific deletion of Cdc42 results in delayed liver regeneration after partial hepatectomy in mice

    DEFF Research Database (Denmark)

    Yuan, Haixin; Zhang, Hong; Wu, Xunwei

    2009-01-01

    Cdc42, a member of the Rho guanosine triphosphatase (GTPase) family, plays important roles in the regulation of the cytoskeleton, cell proliferation, cell polarity, and cellular transport, but little is known about its specific function in mammalian liver. We investigated the function of Cdc42 in...

  13. 77 FR 34046 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2012-06-08

    ... Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES... ACD, CDC, regarding a broad range of public health ethics questions and issues arising from programs... ethics standards to the accreditation process for public health departments; ethical considerations...

  14. 75 FR 72831 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2010-11-26

    ... Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES... counsel to the ACD, CDC, regarding a broad range of public health ethics questions and issues arising from... strategy for addressing its charge to provide a preliminary overview to the ACD on ethical issues related...

  15. 77 FR 2549 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2012-01-18

    ... Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES... will provide counsel to the ACD, CDC, regarding a broad range of public health ethics questions and... territorial health departments in their efforts to address public health ethics challenges, approaches for...

  16. 76 FR 29755 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2011-05-23

    ... Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES... of public health ethics questions and issues arising from programs, scientists and practitioners... April 28, 2011, ACD, CDC meeting; discussion of next steps on addressing potential public health ethical...

  17. 75 FR 4830 - Advisory Committee to the Director (ACD), Centers for Disease Control (CDC) and Prevention-Ethics...

    Science.gov (United States)

    2010-01-29

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control (CDC) and Prevention--Ethics Subcommittee (ES..., CDC, regarding a broad range of public health ethics questions and issues arising from programs...

  18. 75 FR 57044 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2010-09-17

    ... Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES... will provide counsel to the ACD, CDC, regarding a broad range of public health ethics questions and...; efforts to support state, tribal, local and territorial health departments address ethical issues in the...

  19. 75 FR 7483 - Advisory Committee to the Director (ACD), Centers for Disease Control (CDC) and Prevention-Ethics...

    Science.gov (United States)

    2010-02-19

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control (CDC) and Prevention--Ethics Subcommittee (ES); Correction AGENCY: Centers for Disease Control and Prevention (CDC), HHS. ACTION: Notice of meeting; meeting...

  20. 76 FR 55678 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2011-09-08

    ... Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES... provide counsel to the ACD, CDC, regarding a broad range of public health ethics questions and issues... in their efforts to address public health ethics challenges. The agenda is subject to change as...

  1. El sistema acusatorio oral en Costa Rica

    OpenAIRE

    Hernández Barrantes, Jessica María; Rodríguez Montoya, Carmen María; Tenorio Jara

    2014-01-01

    El 1.° de enero de 1998 entra en vigencia el nuevo Código Procesal Penal, con lo cual se experimenta una de las reformas más importantes en materia de Administración de Justicia Penal, que implica el paso de un Sistema Procesal Inquisitivo a un Sistema Acusatorio que procura la adversarialidad entre partes. El antiguo Sistema Penal Inquisitivo se caracterizaba en que las funciones de investigar y juzgar se ejercían por el Juez de instrucción, el proceso era escrito y las dil...

  2. Proyecto seguridad en redes y sistemas

    OpenAIRE

    Valencia Zurera, Miguel

    2016-01-01

    Hoy en día la mayoría de servicios Web son vulnerables a ataques tanto externos como internos y el uso de sistemas de detección de intrusos se hace aconsejable, sino imprescindible, para la prevención de estos ataques. Un IDS es una herramienta de seguridad que intenta detectar o monitorizar los eventos ocurridos en un determinado sistema informático en busca de intentos de comprometer la seguridad de dicho sistema. En este trabajo se realizará una instalación de un IDS basado en Snort para p...

  3. Redundant and nonredundant roles for Cdc42 and Rac1 in lymphomas developed in NPM-ALK transgenic mice

    DEFF Research Database (Denmark)

    Choudhari, Ramesh; Minero, Valerio Giacomo; Menotti, Matteo

    2016-01-01

    Increasing evidence suggests that Rho family GTPases could have a critical role in the biology of T-cell lymphoma. In ALK-rearranged anaplastic large cell lymphoma (ALCL), a specific subtype of T-cell lymphoma, the Rho family GTPases Cdc42 and Rac1 are activated by the ALK oncogenic activity....... In vitro studies have shown that Cdc42 and Rac1 control rather similar phenotypes of ALCL biology such as the proliferation, survival, and migration of lymphoma cells. However, their role and possible redundancy in ALK-driven lymphoma development in vivo are still undetermined. We genetically deleted Cdc42...... or Rac1 in a mouse model of ALK-rearranged ALCL to show that either Cdc42 or Rac1 deletion impaired lymphoma development, modified lymphoma morphology, actin filament distribution, and migration properties of lymphoma cells. Cdc42 or Rac1 deletion primarily affected survival rather than proliferation...

  4. Divergent functions of the Rho GTPases Rac1 and Cdc42 in podocyte injury

    DEFF Research Database (Denmark)

    Blattner, Simone M; Hodgin, Jeffrey B; Nishio, Masashi

    2013-01-01

    effacement, and glomerulosclerosis beginning as early as 10 days of age. In addition, slit diaphragm proteins nephrin and podocin were redistributed, and cofilin was dephosphorylated. Cdc42 is necessary for the maintenance of podocyte structure and function, but Rac1 is entirely dispensable in physiological...

  5. Cdc42/N-WASP signaling links actin dynamics to pancreatic beta cell delamination and differentiation

    DEFF Research Database (Denmark)

    Kesavan, Gokul; Lieven, Oliver; Mamidi, Anant

    2014-01-01

    Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination and epithelial cell movement within the plane of an epithelium are associated with a change in cellular phenotype. However, how this positional change is linked to differentiat......Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination and epithelial cell movement within the plane of an epithelium are associated with a change in cellular phenotype. However, how this positional change is linked...... to differentiation remains unknown. Using the developing mouse pancreas as a model system, we show that β cell delamination and differentiation are two independent events, which are controlled by Cdc42/N-WASP signaling. Specifically, we show that expression of constitutively active Cdc42 in β cells inhibits β cell...... constitutively active Cdc42 partially restores both delamination and β cell differentiation. These findings elucidate how junctional actin dynamics via Cdc42/N-WASP signaling cell-autonomously control not only epithelial delamination but also cell differentiation during mammalian organogenesis....

  6. CDC Vital Signs–Zika Virus: Protecting Pregnant Women and Babies

    Centers for Disease Control (CDC) Podcasts

    2017-04-04

    This podcast is based on the April 2017 CDC Vital Signs report. Zika virus infection during pregnancy can cause serious birth defects. Learn how to protect babies from Zika-related health conditions.  Created: 4/4/2017 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 4/4/2017.

  7. 77 FR 58847 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2012-09-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD... Person for More Information: Carmen Villar, MSW, Designated Federal Officer, ACD, CDC, 1600 Clifton Road...

  8. 77 FR 19018 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2012-03-29

    ... [Federal Register Volume 77, Number 61 (Thursday, March 29, 2012)] [Notices] [Page 19018] [FR Doc No: 2012-7551] DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) In accordance...

  9. 78 FR 60876 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2013-10-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) In accordance with section 10(a)(2) of the Federal...

  10. 76 FR 20354 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2011-04-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD.... Contact Person for More Information: Carmen Villar, MSW, Designated Federal Officer, ACD, CDC, 1600...

  11. 78 FR 64503 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2013-10-29

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) Cancellation: This notice was published in the Federal...

  12. 78 FR 18602 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2013-03-27

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) In accordance with section 10(a)(2) of the Federal...

  13. 76 FR 62071 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2011-10-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) In accordance with section 10(a)(2) of the Federal...

  14. 75 FR 7606 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC...

    Science.gov (United States)

    2010-02-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD... Information: Anne C. Haddix, PhD, Designated Federal Officer, ACD, CDC, 1600 Clifton Road, NE., M/S D14...

  15. Continuous cell injury promotes hepatic tumorigenesis in cdc42-deficient mouse liver

    DEFF Research Database (Denmark)

    van Hengel, Jolanda; D'Hooge, Petra; Hooghe, Bart

    2008-01-01

    : Mice lacking Cdc42 in their hepatocytes were born at Mendelian ratios. They did not show increased mortality but showed chronic jaundice. They developed hepatomegaly soon after birth, and signs of liver transformation, such as formation of nodules and tumors, became visible macroscopically at age 6...

  16. 77 FR 72868 - The Centers for Disease Control (CDC)/Health Resources and Services Administration (HRSA...

    Science.gov (United States)

    2012-12-06

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention The Centers for Disease Control (CDC)/Health Resources and Services Administration (HRSA) Advisory Committee on HIV, Viral... announcements of meetings and other committee management activities, for both the Centers for Disease Control...

  17. CDC Vital Signs–Think Sepsis. Time Matters.

    Centers for Disease Control (CDC) Podcasts

    2016-08-23

    This podcast is based on the August 2016 CDC Vital Signs report. Sepsis is a medical emergency and can happen quickly. Learn the signs of sepsis and how to prevent it.  Created: 8/23/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 8/23/2016.

  18. Differential impact of diverse anticancer chemotherapeutics on the Cdc25A-degradation checkpoint pathway

    International Nuclear Information System (INIS)

    Agner, Jeppe; Falck, Jacob; Lukas, Jiri; Bartek, Jiri

    2005-01-01

    When exposed to DNA-damaging insults such as ionizing radiation (IR) or ultraviolet light (UV), mammalian cells activate checkpoint pathways to halt cell cycle progression or induce cell death. Here we examined the ability of five commonly used anticancer drugs with different mechanisms of action to activate the Chk1/Chk2-Cdc25A-CDK2/cyclin E cell cycle checkpoint pathway, previously shown to be induced by IR or UV. Whereas exposure of human cells to topoisomerase inhibitors camptothecin, etoposide, or adriamycin resulted in rapid (within 1 h) activation of the pathway including degradation of the Cdc25A phosphatase and inhibition of cyclin E/CDK2 kinase activity, taxol failed to activate this checkpoint even after a prolonged treatment. Unexpectedly, although the alkylating agent cisplatin also induced degradation of Cdc25A (albeit delayed, after 8-12 h), cyclin E/CDK2 activity was elevated and DNA synthesis continued, a phenomena that correlated with increased E2F1 protein levels and consequently enhanced expression of cyclin E. These results reveal a differential impact of various classes of anticancer chemotherapeutics on the Cdc25A-degradation pathway, and indicate that the kinetics of checkpoint induction, and the relative balance of key components within the DNA damage response network may dictate whether the treated cells arrest their cell cycle progression

  19. Multiple alterations of platelet functions dominated by increased secretion in mice lacking Cdc42 in platelets

    DEFF Research Database (Denmark)

    Pleines, Irina; Eckly, Anita; Elvers, Margitta

    2010-01-01

    Platelet activation at sites of vascular injury is crucial for hemostasis, but it may also cause myocardial infarction or stroke. Cytoskeletal reorganization is essential for platelet activation and secretion. The small GTPase Cdc42 has been implicated as an important mediator of filopodia...

  20. Poverty and health among CDC plantation labourers in Cameroon: Perceptions, challenges and coping strategies

    NARCIS (Netherlands)

    Makoge, Valerie; Vaandrager, Lenneke; Maat, Harro; Koelen, Maria

    2017-01-01

    Creating better access to good quality healthcare for the poor is a major challenge to development. In this study, we examined inter-linkages between poverty and disease, referred to as poverty-related diseases (PRDs), by investigating how Cameroon Development Corporation (CDC) camp dwellers respond

  1. Cdc42 regulates epithelial cell polarity and cytoskeletal function during kidney tubule development

    DEFF Research Database (Denmark)

    Elias, Bertha C; Das, Amrita; Parekh, Diptiben V

    2015-01-01

    and maintenance, its exact molecular function in kidney development is not well understood. In this study, we define the specific role of Cdc42 during murine kidney epithelial tubulogenesis by deleting it selectively at the initiation of ureteric bud or metanephric mesenchyme development. Deletion in either...

  2. Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin A

    NARCIS (Netherlands)

    Wolthuis, Rob; Clay-Farrace, Lori; van Zon, Wouter; Yekezare, Mona; Koop, Lars; Ogink, Janneke; Medema, Rene; Pines, Jonathon

    2008-01-01

    Successful mitosis requires the right protein be degraded at the right time. Central to this is the spindle checkpoint that prevents the destruction of securin and cyclin 131 when there are improperly attached chromosomes. The principal target of the checkpoint is Cdc20, which activates the

  3. Abortion surveillance at CDC: creating public health light out of political heat.

    Science.gov (United States)

    Cates, W; Grimes, D A; Schulz, K F

    2000-07-01

    In the late 1960s, states began to liberalize their abortion laws, and a new era in women's health began. Under the leadership of Jack Smith, the Centers for Disease Control and Prevention (CDC) established a voluntary abortion surveillance system that provided the first nationwide information on the numbers and characteristics of women having abortions. Studies of abortion morbidity done by the CDC revealed that suction curettage was safer than sharp curettage, local anesthesia was safer than general anesthesia, free-standing clinics were safer than hospitals, and dilation and evacuation (D&E) was safer than the alternative of labor induction for early second-trimester abortions. This evidence, which contradicted traditional medical tenets, rapidly changed the practice of abortion in the United States. CDC also established a surveillance system for abortion deaths. This demonstrated a rapid improvement in the safety of abortion in the early 1970s. Lessons learned from mortality investigations helped to change practice as well.Today, more is known about the epidemiology of abortion than any other operation in the history of medicine. In the midst of strident debate over the abortion issue, CDC abortion surveillance data have helped to guide judicial rulings, legislative actions, and Surgeon General's reports, which have supported safer choices for women of reproductive age. When medical historians of the future look back on this century, the increasing availability of safe, legal abortion will stand out as a public health triumph.

  4. Cdc42/N-WASP signaling links actin dynamics to pancreatic β cell delamination and differentiation

    Science.gov (United States)

    Kesavan, Gokul; Lieven, Oliver; Mamidi, Anant; Öhlin, Zarah Löf; Johansson, Jenny Kristina; Li, Wan-Chun; Lommel, Silvia; Greiner, Thomas Uwe; Semb, Henrik

    2014-01-01

    Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination and epithelial cell movement within the plane of an epithelium are associated with a change in cellular phenotype. However, how this positional change is linked to differentiation remains unknown. Using the developing mouse pancreas as a model system, we show that β cell delamination and differentiation are two independent events, which are controlled by Cdc42/N-WASP signaling. Specifically, we show that expression of constitutively active Cdc42 in β cells inhibits β cell delamination and differentiation. These processes are normally associated with junctional actin and cell-cell junction disassembly and the expression of fate-determining transcription factors, such as Isl1 and MafA. Mechanistically, we demonstrate that genetic ablation of N-WASP in β cells expressing constitutively active Cdc42 partially restores both delamination and β cell differentiation. These findings elucidate how junctional actin dynamics via Cdc42/N-WASP signaling cell-autonomously control not only epithelial delamination but also cell differentiation during mammalian organogenesis. PMID:24449844

  5. Using Evidence-Based Parenting Programs to Advance CDC Efforts in Child Maltreatment Prevention. Research Brief

    Science.gov (United States)

    Valle, Linda Anne; Whitaker, Daniel J.; Lutzker, John R.; Filene, Jill H.; Wyatt, Jennifer M.; Cephas, Kendell C.; Hoover, D. Michele

    2004-01-01

    The Centers for Disease Control and Prevention (CDC) recognize child maltreatment as a serious public health problem with extensive short- and long-term health effects. In addition to the immediate physical and emotional effects of maltreatment, children who have experienced abuse and neglect are at increased risk of adverse health effects and…

  6. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1

    NARCIS (Netherlands)

    Lee, S.B.; Kim, J.J.; Nam, H.J.; Gao, B.; Yin, P.; Qin, B.; Yi, S.Y.; Ham, H.; Evans, D.; Kim, S.H.; Zhang, J.; Deng, M.; Liu, T.; Zhang, H.; Billadeau, D.D.; Wang, L.; Giaime, E.; Shen, J.; Pang, Y.P.; Jen, J.; Deursen, J.M.A. van; Lou, Z.

    2015-01-01

    Mutations in the E3 ubiquitin ligase Parkin have been linked to familial Parkinson's disease. Parkin has also been implicated in mitosis through mechanisms that are unclear. Here we show that Parkin interacts with anaphase promoting complex/cyclosome (APC/C) coactivators Cdc20 and Cdh1 to mediate

  7. Diabetes: What’s Your Type? (A Cup of Health with CDC)

    Centers for Disease Control (CDC) Podcasts

    2018-03-29

    Diabetes is a common chronic disease and the seventh leading cause of death in the U.S. The CDC estimates that about 30 million people in the United States have diabetes. In this podcast, Dr. Stephen Benoit discusses the importance of managing diabetes.  Created: 3/29/2018 by MMWR.   Date Released: 3/29/2018.

  8. Diabetes: What’s Your Type? (A Minute of Health with CDC)

    Centers for Disease Control (CDC) Podcasts

    2018-03-29

    Diabetes is a common chronic disease and the seventh leading cause of death in the U.S. The CDC estimates that about 30 million people in the United States have diabetes. In this podcast, Dr. Stephen Benoit discusses the importance of managing diabetes.  Created: 3/29/2018 by MMWR.   Date Released: 3/29/2018.

  9. CDC Vital Signs–Cancer and Tobacco Use

    Centers for Disease Control (CDC) Podcasts

    2016-11-10

    This podcast is based on the November 2016 CDC Vital Signs report. There is a long list of cancers linked to tobacco use, the leading preventable cause of cancer and cancer deaths. Learn more here.  Created: 11/10/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 11/10/2016.

  10. Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin

    DEFF Research Database (Denmark)

    Woodham, Emma F; Paul, Nikki R; Tyrrell, Benjamin

    2017-01-01

    The individual molecular pathways downstream of Cdc42, Rac, and Rho GTPases are well documented, but we know surprisingly little about how these pathways are coordinated when cells move in a complex environment in vivo. In the developing embryo, melanoblasts originating from the neural crest must...... null cells lacked the ability to polarize their Golgi and coordinate motility systems for efficient movement. Loss of Cdc42 de-coupled three main systems: actin assembly via the formin FMNL2 and Arp2/3, active myosin-II localization, and integrin-based adhesion dynamics.......The individual molecular pathways downstream of Cdc42, Rac, and Rho GTPases are well documented, but we know surprisingly little about how these pathways are coordinated when cells move in a complex environment in vivo. In the developing embryo, melanoblasts originating from the neural crest must...... traverse the dermis to reach the epidermis of the skin and hair follicles. We previously established that Rac1 signals via Scar/WAVE and Arp2/3 to effect pseudopod extension and migration of melanoblasts in skin. Here we show that RhoA is redundant in the melanocyte lineage but that Cdc42 coordinates...

  11. 77 FR 23733 - CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment

    Science.gov (United States)

    2012-04-20

    ... Resources and Services Administration CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment In... and control of HIV/AIDS and other STDs, the support of health care services to persons living with HIV/AIDS, and education of health professionals and the public about HIV/AIDS and other STDs. Matters To Be...

  12. 75 FR 39264 - CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment

    Science.gov (United States)

    2010-07-08

    ... Resources and Services Administration CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment In... and control of HIV/AIDS and other STDs, the support of health care services to persons living with HIV/AIDS, and education of health professionals and the public about HIV/AIDS and other STDs. Matters To Be...

  13. FMNL2 and -3 regulate Golgi architecture and anterograde transport downstream of Cdc42

    DEFF Research Database (Denmark)

    Kage, Frieda; Steffen, Anika; Ellinger, Adolf

    2017-01-01

    with Cdc42. Moreover, Golgi association of FMNL2 or -3 induced a phalloidin-detectable actin meshwork around the Golgi. Importantly, functional interference with FMNL2/3 formins by RNAi or CRISPR/Cas9-mediated gene deletion invariably induced Golgi fragmentation in different cell lines. Furthermore...

  14. Comparing U.S. Injury Death Estimates from GBD 2015 and CDC WONDER

    Directory of Open Access Journals (Sweden)

    Yue Wu

    2018-01-01

    Full Text Available Objective: The purpose of the present study was to examine consistency in injury death statistics from the United States CDC Wide-ranging Online Data for Epidemiologic Research (CDC WONDER with those from GBD 2015 estimates. Methods: Differences in deaths and the percent difference in deaths between GBD 2015 and CDC WONDER were assessed, as were changes in deaths between 2000 and 2015 for the two datasets. Results: From 2000 to 2015, GBD 2015 estimates for the U.S. injury deaths were somewhat higher than CDC WONDER estimates in most categories, with the exception of deaths from falls and from forces of nature, war, and legal intervention in 2015. Encouragingly, the difference in total injury deaths between the two data sources narrowed from 44,897 (percent difference in deaths = 41% in 2000 to 34,877 (percent difference in deaths = 25% in 2015. Differences in deaths and percent difference in deaths between the two data sources varied greatly across injury cause and over the assessment years. The two data sources present consistent changes in direction from 2000 to 2015 for all injury causes except for forces of nature, war, and legal intervention, and adverse effects of medical treatment. Conclusions: We conclude that further studies are warranted to interpret the inconsistencies in data and develop estimation approaches that increase the consistency of the two datasets.

  15. Isolation of a cdc28 mutation that abrogates the dependence of S ...

    Indian Academy of Sciences (India)

    Unknown

    mented or no DNA (Enoch and Nurse 1990). In S. cere- visiae also, a mutation in this gene abrogates the cell- cycle arrest caused by DNA damage (Li and Cai 1997). In the presence of this mutation, cdc13 cells undergo nu- clear division and complete a round of cell cycle at the nonpermissive temperature. Dahmann et al.

  16. A novel functional polymorphism in the Cdc6 promoter is associated with the risk for hepatocellular carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Xiong Xingdong; Fang Jianhong; Qiu Fuen; Zhao Jing; Cheng Jiasen [Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou (China); Yuan Yunfei; Li Shengping [State Key Laboratory of Oncology in Southern China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Zhuang Shimei [Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou (China); State Key Laboratory of Oncology in Southern China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)], E-mail: LSSZSM@mail.sysu.edu.cn

    2008-08-25

    Cdc6 is essential for DNA replication and its deregulation is involved in carcinogenesis. To date, the biological significance of the polymorphism in Cdc6 promoter is still unknown. In this study, we aimed to evaluate the influence of the Cdc6 -515A>G polymorphism (rs4134994) on the individual's susceptibility to cancer and on the function of Cdc6. The Cdc6 -515A>G polymorphism was genotyped in 387 hepatocellular carcinoma (HCC) and 389 age- and sex-matched healthy subjects. The association between the genotypes and the risk for HCC was then estimated by unconditional logistic regression analysis with adjustment for age, sex and HBV status. Compared with the AA homozygotes, the homozygous GG genotype (adjusted OR = 0.36, 95% confidence interval (CI) = 0.18-0.72, P = 0.004) or the combined AG/GG genotypes (adjusted OR = 0.56, 95% CI = 0.36-0.86, P = 0.008) were statistically significantly associated with the reduced risk for HCC. Moreover, the analysis using luciferase reporter system showed that the G-allelic Cdc6 promoter displayed a decreased transcriptional activity compared with the A-allelic one. These results indicate that the individuals with G allele may have reduced Cdc6 expression and are therefore in reduced risk for HCC. Further investigation using electrophoretic mobility shift assay (EMSA) revealed that the G allele had a stronger binding strength to nuclear protein(s) which might function as negative regulator(s) for Cdc6 transcription. Our findings suggest that the -515A>G polymorphism may affect the Cdc6 promoter binding affinity with nuclear protein(s) and in turn the Cdc6 expression, which consequently modulates the individual's susceptibility to HCC.

  17. A novel functional polymorphism in the Cdc6 promoter is associated with the risk for hepatocellular carcinoma

    International Nuclear Information System (INIS)

    Xiong Xingdong; Fang Jianhong; Qiu Fuen; Zhao Jing; Cheng Jiasen; Yuan Yunfei; Li Shengping; Zhuang Shimei

    2008-01-01

    Cdc6 is essential for DNA replication and its deregulation is involved in carcinogenesis. To date, the biological significance of the polymorphism in Cdc6 promoter is still unknown. In this study, we aimed to evaluate the influence of the Cdc6 -515A>G polymorphism (rs4134994) on the individual's susceptibility to cancer and on the function of Cdc6. The Cdc6 -515A>G polymorphism was genotyped in 387 hepatocellular carcinoma (HCC) and 389 age- and sex-matched healthy subjects. The association between the genotypes and the risk for HCC was then estimated by unconditional logistic regression analysis with adjustment for age, sex and HBV status. Compared with the AA homozygotes, the homozygous GG genotype (adjusted OR = 0.36, 95% confidence interval (CI) = 0.18-0.72, P = 0.004) or the combined AG/GG genotypes (adjusted OR = 0.56, 95% CI = 0.36-0.86, P = 0.008) were statistically significantly associated with the reduced risk for HCC. Moreover, the analysis using luciferase reporter system showed that the G-allelic Cdc6 promoter displayed a decreased transcriptional activity compared with the A-allelic one. These results indicate that the individuals with G allele may have reduced Cdc6 expression and are therefore in reduced risk for HCC. Further investigation using electrophoretic mobility shift assay (EMSA) revealed that the G allele had a stronger binding strength to nuclear protein(s) which might function as negative regulator(s) for Cdc6 transcription. Our findings suggest that the -515A>G polymorphism may affect the Cdc6 promoter binding affinity with nuclear protein(s) and in turn the Cdc6 expression, which consequently modulates the individual's susceptibility to HCC

  18. Comparação entre os modelos norte-americano (ACSI) e europeu (ECSI) de satisfação do cliente: um estudo no setor de serviços

    OpenAIRE

    Lopes, Humberto Elias Garcia; Pereira, Camila Cristina de Paula; Vieira, Ana Flávia Santos

    2009-01-01

    O objetivo central deste trabalho é comparar os modelos norte-americano (ACSI) e europeu (ECSI) de satisfação do cliente, preservando as relações estruturais entre as variáveis latentes tal como originalmente estabelecidas. Para tanto, optou-se por uma abordagem quantitativa, centrada na modelagem de equações estruturais por meio da análise fatorial confirmatória. Foram pesquisados 2.145 clientes de empresas do setor de serviços, atuantes no Estado de Minas Gerais. Os principais resultados in...

  19. A produção científica em Segurança Alimentar e Nutricional nos periódicos Latino-Americanos da base de dados SciELO

    OpenAIRE

    Gomes, Rogério

    2015-01-01

    Trabalho de Conclusão de Curso apresentado ao Instituto Latino-Americano de Economia, Sociedade e Política da Universidade Federal da Integração Latino-Americana, como requisito parcial à obtenção do título de Bacharel em Desenvolvimento Rural e Segurança Alimentar. Orientadora: Profa. Dra. Érika Marafon Rodrigues Ciacchi. Co-orientadora: Profa. Dra. Silvia Aparecida Zimmermman. O objetivo da pesquisa foi analisar trabalhos publicados nos países da América Latina que compõe o conjunto ...

  20. JUAN M. LOPE BLANCH. El habla de Diego de Ordaz. Contribución a la historia del español americano. México, UNAM. 1985. 233 p.

    Directory of Open Access Journals (Sweden)

    J. L. R.

    1988-06-01

    Full Text Available Este libro reúne los trabajos publicados por Lope Blanch en revistas especializadas y homenajes académicos acerca del idiolecto de este importante americano nacido en tierras leonesas hacia 1480, colonizador de las Antillas, expedicionario a Tierra Firme y a Cuba, conquistador de México y explorador del Orinoco. La base documental está constituida por siete cartas autógrafasque L.B. publica al final del libro en una cuidadosa transcripción que corrige defectos de una edición anterior.

  1. Heart transplant outcomes in recipients of Centers for Disease Control (CDC) high risk donors.

    Science.gov (United States)

    Tsiouris, Athanasios; Wilson, Lynn; Sekar, Rajesh B; Mangi, Abeel A; Yun, James J

    2016-12-01

    A lack of donor hearts remains a major limitation of heart transplantation. Hearts from Centers for Disease Control (CDC) high-risk donors can be utilized with specific recipient consent. However, outcomes of heart transplantation with CDC high-risk donors are not well known. We sought to define outcomes, including posttransplant hepatitis and human immunodeficiency virus (HIV) status, in recipients of CDC high-risk donor hearts at our institution. All heart transplant recipients from August 2010 to December 2014 (n = 74) were reviewed. Comparison of 1) CDC high-risk donor (HRD) versus 2) standard-risk donor (SRD) groups were performed using chi-squared tests for nominal data and Wilcoxon two-sample tests for continuous variables. Survival was estimated with Kaplan-Meier curves. Of 74 heart transplant recipients reviewed, 66 (89%) received a SRD heart and eight (11%) received a CDC HRD heart. We found no significant differences in recipient age, sex, waiting list 1A status, pretransplant left ventricular assist device (LVAD) support, cytomegalovirus (CMV) status, and graft ischemia times (p = NS) between the HRD and SRD groups. All of the eight HRD were seronegative at the time of transplant. Postoperatively, there was no significant difference in rejection rates at six and 12 months posttransplant. Importantly, no HRD recipients acquired hepatitis or HIV. Survival in HRD versus SRD recipients was not significantly different by Kaplan-Meier analysis (log rank p = 0.644) at five years posttransplant. Heart transplants that were seronegative at the time of transplant had similar posttransplant graft function, rejection rates, and five-year posttransplant survival versus recipients of SRD hearts. At our institution, no cases of hepatitis or HIV occurred in HRD recipients in early follow-up. © 2016 Wiley Periodicals, Inc.

  2. Cdc20 is critical for meiosis I and fertility of female mice.

    Directory of Open Access Journals (Sweden)

    Fang Jin

    2010-09-01

    Full Text Available Chromosome missegregation in germ cells is an important cause of unexplained infertility, miscarriages, and congenital birth defects in humans. However, the molecular defects that lead to production of aneuploid gametes are largely unknown. Cdc20, the activating subunit of the anaphase-promoting complex/cyclosome (APC/C, initiates sister-chromatid separation by ordering the destruction of two key anaphase inhibitors, cyclin B1 and securin, at the transition from metaphase to anaphase. The physiological significance and full repertoire of functions of mammalian Cdc20 are unclear at present, mainly because of the essential nature of this protein in cell cycle progression. To bypass this problem we generated hypomorphic mice that express low amounts of Cdc20. These mice are healthy and have a normal lifespan, but females produce either no or very few offspring, despite normal folliculogenesis and fertilization rates. When mated with wild-type males, hypomorphic females yield nearly normal numbers of fertilized eggs, but as these embryos develop, they become malformed and rarely reach the blastocyst stage. In exploring the underlying mechanism, we uncover that the vast majority of these embryos have abnormal chromosome numbers, primarily due to chromosome lagging and chromosome misalignment during meiosis I in the oocyte. Furthermore, cyclin B1, cyclin A2, and securin are inefficiently degraded in metaphase I; and anaphase I onset is markedly delayed. These results demonstrate that the physiologically effective threshold level of Cdc20 is high for female meiosis I and identify Cdc20 hypomorphism as a mechanism for chromosome missegregation and formation of aneuploid gametes.

  3. Cdc15 Phosphorylates the C-terminal Domain of RNA Polymerase II for Transcription during Mitosis.

    Science.gov (United States)

    Singh, Amit Kumar; Rastogi, Shivangi; Shukla, Harish; Asalam, Mohd; Rath, Srikanta Kumar; Akhtar, Md Sohail

    2017-03-31

    In eukaryotes, the basal transcription in interphase is orchestrated through the regulation by kinases (Kin28, Bur1, and Ctk1) and phosphatases (Ssu72, Rtr1, and Fcp1), which act through the post-translational modification of the C-terminal domain (CTD) of the largest subunit of RNA polymerase II. The CTD comprises the repeated Tyr-Ser-Pro-Thr-Ser-Pro-Ser motif with potential epigenetic modification sites. Despite the observation of transcription and periodic expression of genes during mitosis with entailing CTD phosphorylation and dephosphorylation, the associated CTD specific kinase(s) and its role in transcription remains unknown. Here we have identified Cdc15 as a potential kinase phosphorylating Ser-2 and Ser-5 of CTD for transcription during mitosis in the budding yeast. The phosphorylation of CTD by Cdc15 is independent of any prior Ser phosphorylation(s). The inactivation of Cdc15 causes reduction of global CTD phosphorylation during mitosis and affects the expression of genes whose transcript levels peak during mitosis. Cdc15 also influences the complete transcription of clb2 gene and phosphorylates Ser-5 at the promoter and Ser-2 toward the 3' end of the gene. The observation that Cdc15 could phosphorylate Ser-5, as well as Ser-2, during transcription in mitosis is in contrast to the phosphorylation marks put by the kinases in interphase (G 1 , S, and G 2 ), where Cdck7/Kin28 phosphorylates Ser-5 at promoter and Bur1/Ctk1 phosphorylates Ser-2 at the 3' end of the genes. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  4. Cdc15 Phosphorylates the C-terminal Domain of RNA Polymerase II for Transcription during Mitosis*

    Science.gov (United States)

    Singh, Amit Kumar; Rastogi, Shivangi; Shukla, Harish; Asalam, Mohd.; Rath, Srikanta Kumar; Akhtar, Md. Sohail

    2017-01-01

    In eukaryotes, the basal transcription in interphase is orchestrated through the regulation by kinases (Kin28, Bur1, and Ctk1) and phosphatases (Ssu72, Rtr1, and Fcp1), which act through the post-translational modification of the C-terminal domain (CTD) of the largest subunit of RNA polymerase II. The CTD comprises the repeated Tyr-Ser-Pro-Thr-Ser-Pro-Ser motif with potential epigenetic modification sites. Despite the observation of transcription and periodic expression of genes during mitosis with entailing CTD phosphorylation and dephosphorylation, the associated CTD specific kinase(s) and its role in transcription remains unknown. Here we have identified Cdc15 as a potential kinase phosphorylating Ser-2 and Ser-5 of CTD for transcription during mitosis in the budding yeast. The phosphorylation of CTD by Cdc15 is independent of any prior Ser phosphorylation(s). The inactivation of Cdc15 causes reduction of global CTD phosphorylation during mitosis and affects the expression of genes whose transcript levels peak during mitosis. Cdc15 also influences the complete transcription of clb2 gene and phosphorylates Ser-5 at the promoter and Ser-2 toward the 3′ end of the gene. The observation that Cdc15 could phosphorylate Ser-5, as well as Ser-2, during transcription in mitosis is in contrast to the phosphorylation marks put by the kinases in interphase (G1, S, and G2), where Cdck7/Kin28 phosphorylates Ser-5 at promoter and Bur1/Ctk1 phosphorylates Ser-2 at the 3′ end of the genes. PMID:28202544

  5. Cdc42 and Tks5: a minimal and universal molecular signature for functional invadosomes.

    Science.gov (United States)

    Di Martino, Julie; Paysan, Lisa; Gest, Caroline; Lagrée, Valérie; Juin, Amélie; Saltel, Frédéric; Moreau, Violaine

    2014-01-01

    Invadosomes are actin-based structures involved in extracellular-matrix degradation. Invadosomes, either known as podosomes or invadopodia, are found in an increasing number of cell types. Moreover, their overall organization and molecular composition may vary from one cell type to the other. Some are constitutive such as podosomes in hematopoietic cells whereas others are inducible. However, they share the same feature, their ability to interact and to degrade the extracellular matrix. Based on the literature and our own experiments, the aim of this study was to establish a minimal molecular definition of active invadosomes. We first highlighted that Cdc42 is the key RhoGTPase involved in invadosome formation in all described models. Using different cellular models, such as NIH-3T3, HeLa, and endothelial cells, we demonstrated that overexpression of an active form of Cdc42 is sufficient to form invadosome actin cores. Therefore, active Cdc42 must be considered not only as an inducer of filopodia, but also as an inducer of invadosomes. Depending on the expression level of Tks5, these Cdc42-dependent actin cores were endowed or not with a proteolytic activity. In fact, Tks5 overexpression rescued this activity in Tks5 low expressing cells. We thus described the adaptor protein Tks5 as a major actor of the invadosome degradation function. Surprisingly, we found that Src kinases are not always required for invadosome formation and function. These data suggest that even if Src family members are the principal kinases involved in the majority of invadosomes, it cannot be considered as a common element for all invadosome structures. We thus define a minimal and universal molecular signature of invadosome that includes Cdc42 activity and Tks5 presence in order to drive the actin machinery and the proteolytic activity of these invasive structures.

  6. Diversity and evolution of multiple orc/cdc6-adjacent replication origins in haloarchaea

    Directory of Open Access Journals (Sweden)

    Wu Zhenfang

    2012-09-01

    Full Text Available Abstract Background While multiple replication origins have been observed in archaea, considerably less is known about their evolutionary processes. Here, we performed a comparative analysis of the predicted (proved in part orc/cdc6-associated replication origins in 15 completely sequenced haloarchaeal genomes to investigate the diversity and evolution of replication origins in halophilic Archaea. Results Multiple orc/cdc6-associated replication origins were predicted in all of the analyzed haloarchaeal genomes following the identification of putative ORBs (origin recognition boxes that are associated with orc/cdc6 genes. Five of these predicted replication origins in Haloarcula hispanica were experimentally confirmed via autonomous replication activities. Strikingly, several predicted replication origins in H. hispanica and Haloarcula marismortui are located in the distinct regions of their highly homologous chromosomes, suggesting that these replication origins might have been introduced as parts of new genomic content. A comparison of the origin-associated Orc/Cdc6 homologs and the corresponding predicted ORB elements revealed that the replication origins in a given haloarchaeon are quite diverse, while different haloarchaea can share a few conserved origins. Phylogenetic and genomic context analyses suggested that there is an original replication origin (oriC1 that was inherited from the ancestor of archaea, and several other origins were likely evolved and/or translocated within the haloarchaeal species. Conclusion This study provides detailed information about the diversity of multiple orc/cdc6-associated replication origins in haloarchaeal genomes, and provides novel insight into the evolution of multiple replication origins in Archaea.

  7. A TOCA/CDC-42/PAR/WAVE functional module required for retrograde endocytic recycling

    Science.gov (United States)

    Bai, Zhiyong; Grant, Barth D.

    2015-01-01

    Endosome-to-Golgi transport is required for the function of many key membrane proteins and lipids, including signaling receptors, small-molecule transporters, and adhesion proteins. The retromer complex is well-known for its role in cargo sorting and vesicle budding from early endosomes, in most cases leading to cargo fusion with the trans-Golgi network (TGN). Transport from recycling endosomes to the TGN has also been reported, but much less is understood about the molecules that mediate this transport step. Here we provide evidence that the F-BAR domain proteins TOCA-1 and TOCA-2 (Transducer of Cdc42 dependent actin assembly), the small GTPase CDC-42 (Cell division control protein 42), associated polarity proteins PAR-6 (Partitioning defective 6) and PKC-3/atypical protein kinase C, and the WAVE actin nucleation complex mediate the transport of MIG-14/Wls and TGN-38/TGN38 cargo proteins from the recycling endosome to the TGN in Caenorhabditis elegans. Our results indicate that CDC-42, the TOCA proteins, and the WAVE component WVE-1 are enriched on RME-1–positive recycling endosomes in the intestine, unlike retromer components that act on early endosomes. Furthermore, we find that retrograde cargo TGN-38 is trapped in early endosomes after depletion of SNX-3 (a retromer component) but is mainly trapped in recycling endosomes after depletion of CDC-42, indicating that the CDC-42–associated complex functions after retromer in a distinct organelle. Thus, we identify a group of interacting proteins that mediate retrograde recycling, and link these proteins to a poorly understood trafficking step, recycling endosome-to-Golgi transport. We also provide evidence for the physiological importance of this pathway in WNT signaling. PMID:25775511

  8. Legislativo e política comercial: a aprovação do TLC com os Estados Unidos nos legislativos sul-americanos

    Directory of Open Access Journals (Sweden)

    Pedro Feliú Ribeiro

    2012-11-01

    Full Text Available O artigo analisa as votações nominais de três poderes legislativos sul-americanos, Chile, Colômbia e Peru, acerca da ratificação do Tratado de Livre Comércio com os Estados Unidos. A pergunta central é: quais são os fatores determinantes do voto do legislador na aprovação do Tratado de Livre Comércio (TLC com os Estados Unidos? Três fatores centrais emergem da literatura: (i as relações Estados Unidos x América Latina produzem uma clivagem ideológica (direita-esquerda entre os partidos políticos do continente; (ii o pertencimento do legislador à coalizão de governo no poder Legislativo e (iii os fatores sócio-econômicos dos distritos eleitorais dos legisladores. Por meio da utilização do modelo de regressão logística, apontamos que a ideologia dos legisladores peruanos explica os votos dos mesmos no TLC com os Estados Unidos, indicando que quanto mais à esquerda estiver o legislador, menor a probabilidade do mesmo aprovar o referido tratado. No caso chileno, a taxa de desemprego do distrito eleitoral do deputado possui maior capacidade explicativa, revelando que quanto maior a taxa de desemprego, menor a probabilidade de aprovação do TLC com os Estados Unidos. Já no caso do Senado colombiano, há uma forte associação entre o pertencimento do Senador à coalizão de governo e a aprovação do TLC, revelando a influência dessa variável institucional no voto do Senado colombiano. Além do pertencimento ou não à coalizão de governo, a ideologia do partido político do legislador colombiano também se apresenta como fator explicativo relevante do voto do mesmo.

  9. Cogeneration Systems; Sistemas de Cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez M, Manuel F; Huante P, Liborio; Romo M, Cesar A [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2006-07-01

    The present article deals on relevant aspects on the subject of cogeneration within the Mexican territorial limits. In the first place it is presented the role of Mexico in terms of its cogeneration potential, the type of service that has obtained from this predominant modality of cogeneration for self-supplying, the most propitious sectors to develop it, its legislations on the matter, the projects made for the implementation of cogeneration plants, as well as the existing cogeneration schemes for its respective optimization proposals. Without leaving out the analysis on the different types of evaluation on the efficiency of cogeneration systems and the aspects to consider for the election of a generation cycle. [Spanish] El presente articulo trata sobre aspectos relevantes en materia de cogeneracion dentro de los limites territoriales de la nacion mexicana. Se muestra en primer lugar el papel de Mexico en terminos de su potencial de cogeneracion, el tipo de servicio que ha obtenido de esta predominantemente (modalidad de cogeneracion para autoabastecimiento), los sectores mas propicios para desarrollarla, sus legislaciones al respecto, los proyectos realizados para la implementacion de plantas de cogeneracion, asi como los esquemas de cogeneracion existentes con sus respectivas propuestas de optimizacion. Sin dejar de lado el analisis sobre los distintos tipos de evaluacion de la eficiencia de sistemas de cogeneracion y los aspectos a considerar para la eleccion de un ciclo de generacion.

  10. Cdc42p and Rho1p are sequentially activated and mechanistically linked to vacuole membrane fusion

    International Nuclear Information System (INIS)

    Logan, Michael R.; Jones, Lynden; Eitzen, Gary

    2010-01-01

    Small monomeric GTPases act as molecular switches, regulating many biological functions via activation of membrane localized signaling cascades. Activation of their switch function is controlled by GTP binding and hydrolysis. Two Rho GTPases, Cdc42p and Rho1p, are localized to the yeast vacuole where they regulate membrane fusion. Here, we define a method to directly examine vacuole membrane Cdc42p and Rho1p activation based on their affinity to probes derived from effectors. Cdc42p and Rho1p showed unique temporal activation which aligned with distinct subreactions of in vitro vacuole fusion. Cdc42p was rapidly activated in an ATP-independent manner while Rho1p activation was kinetically slower and required ATP. Inhibitors that are known to block vacuole membrane fusion were examined for their effect on Cdc42p and Rho1p activation. Rdi1p, which inhibits the dissociation of GDP from Rho proteins, blocked both Cdc42p and Rho1p activation. Ligands of PI(4,5)P 2 specifically inhibited Rho1p activation while pre-incubation with U73122, which targets Plc1p function, increased Rho1p activation. These results define unique activation mechanisms for Cdc42p and Rho1p, which may be linked to the vacuole membrane fusion mechanism.

  11. FLIM FRET Visualization of Cdc42 Activation by Netrin-1 in Embryonic Spinal Commissural Neuron Growth Cones.

    Directory of Open Access Journals (Sweden)

    Benjamin Rappaz

    Full Text Available Netrin-1 is an essential extracellular chemoattractant that signals through its receptor DCC to guide commissural axon extension in the embryonic spinal cord. DCC directs the organization of F-actin in growth cones by activating an intracellular protein complex that includes the Rho GTPase Cdc42, a critical regulator of cell polarity and directional migration. To address the spatial distribution of signaling events downstream of netrin-1, we expressed the FRET biosensor Raichu-Cdc42 in cultured embryonic rat spinal commissural neurons. Using FLIM-FRET imaging we detected rapid activation of Cdc42 in neuronal growth cones following application of netrin-1. Investigating the signaling mechanisms that control Cdc42 activation by netrin-1, we demonstrate that netrin-1 rapidly enriches DCC at the leading edge of commissural neuron growth cones and that netrin-1 induced activation of Cdc42 in the growth cone is blocked by inhibiting src family kinase signaling. These findings reveal the activation of Cdc42 in embryonic spinal commissural axon growth cones and support the conclusion that src family kinase activation downstream of DCC is required for Cdc42 activation by netrin-1.

  12. Regulating Cdc42 and Its Signaling Pathways in Cancer: Small Molecules and MicroRNA as New Treatment Candidates.

    Science.gov (United States)

    Xiao, Xing-Hua; Lv, Lin-Chen; Duan, Jing; Wu, Ye-Meng; He, Shu-Jin; Hu, Zhen-Zhen; Xiong, Li-Xia

    2018-03-29

    Despite great improvements in the diagnosis and treatment of neoplasms, metastatic disease is still the leading cause of death in cancer patients, with mortality rates still rising. Given this background, new ways to treat cancer will be important for development of improved cancer control strategies. Cdc42 is a member of the Rho GTPase family and plays an important role in cell-to-cell adhesion, formation of cytoskeletal structures, and cell cycle regulation. It thus influences cellular proliferation, transformation, and homeostasis, as well as the cellular migration and invasion processes underlying tumor formation. Cdc42 acts as a collection point for signal transduction and regulates multiple signaling pathways. Moreover, recent studies show that in most human cancers Cdc42 is abnormally expressed and promoting neoplastic growth and metastasis. Regarding possible new treatments for cancer, miRNA and small molecules targeting Cdc42 and related pathways have been recently found to be effective on cancer. In this review, we analyze the newly recognized regulation mechanisms for Cdc42 and Cdc42-related signal pathways, and particularly new treatments using small molecules and miRNAs to inhibit the abnormal overexpression of Cdc42 that may slow down the metastasis process, improve cancer therapy and lead to novel strategies for development of antineoplastic drugs.

  13. Cdc42 and RhoA reveal different spatio-temporal dynamics upon local stimulation with Semaphorin-3A

    Directory of Open Access Journals (Sweden)

    Federico eIseppon

    2015-08-01

    Full Text Available Small RhoGTPases, such as Cdc42 and RhoA, are key players in integrating external cues and intracellular signaling pathways that regulate growth cone (GC motility. Indeed, Cdc42 is involved in actin polymerization and filopodia formation, whereas RhoA induces GC collapse and neurite retraction through actomyosin contraction. In this study we employed Förster Resonance Energy Transfer (FRET microscopy to study the spatio-temporal dynamics of Cdc42 and RhoA in GCs in response to local Semaphorin-3A stimulation obtained with lipid vesicles filled with Semaphorin-3A and positioned near the selected GC using optical tweezers. We found that Cdc42 and RhoA were activated at the leading edge of NG108-15 neuroblastoma cells during spontaneous cycles of protrusion and retraction, respectively. The release of Semaphorin-3A brought to a progressive activation of RhoA within 30 seconds from the stimulus in the central region of the GC that collapsed and retracted. In contrast, the same stimulation evoked waves of Cdc42 activation propagating away from the stimulated region. A more localized stimulation obtained with Sema3A coated beads placed on the GC, led to Cdc42 active waves that propagated in a retrograde manner with a mean period of 70 seconds, and followed by GC retraction. Therefore, Semaphorin-3A activates both Cdc42 and RhoA with a complex and different spatial-temporal dynamics.

  14. Inclusión del coeficiente de consanguinidad en los modelos de evaluación genética de bovinos Jersey y Suizo Americano en México

    Directory of Open Access Journals (Sweden)

    Agustín Ruíz-Flores

    2011-01-01

    lactancia de leche (PL, grasa (PG y proteína (PP, y los porcentajes de grasa (%G y proteína (%P en leche; mientras que en Suizo Americano sólo se analizó la PL. Los pedigríes incluyeron 21,026 animales Jersey y 101,861 Suizo Americano. El cambio más notorio correspondió a los valores genéticos predichos (VGP para %P en Jersey, los VGP para las demás características fueron similares en ambas razas. Los porcentajes de coincidencia entre los 100 sementales superiores evaluados con ambos modelos variaron de 90 a 98 %. Los coeficientes de regresión de los VGP y de exactitudes obtenidos con el modelo sin el efecto de la consanguinidad sobre los del modelo con el efecto de consanguinidad fluctuaron alrededor de 1.0 en ambas razas. Los estimados de las varianzas genéticas y de h2 obtenidos con ambos modelos fueron similares para todas las características. No se considera necesaria la inclusión de F en los modelos para evaluar genéticamente las poblaciones estudiadas, sin embargo, se recomienda realizar nuevamente este estudio cuando el promedio de F sea mayor que el actual.

  15. Prominin-2 expression increases protrusions, decreases caveolae and inhibits Cdc42 dependent fluid phase endocytosis

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Raman Deep, E-mail: Takhter.Ramandeep@mayo.edu; Schroeder, Andreas S.; Scheffer, Luana; Holicky, Eileen L.; Wheatley, Christine L.; Marks, David L., E-mail: Marks.david@mayo.edu; Pagano, Richard E.

    2013-05-10

    Highlights: •Prominin-2 expression induced protrusions that co-localized with lipid raft markers. •Prominin-2 expression decreased caveolae, caveolar endocytosis and increased pCav1. •Prominin-2 expression inhibited fluid phase endocytosis by inactivation of Cdc42. •These endocytic effects can be reversed by adding exogenous cholesterol. •Caveolin1 knockdown restored fluid phase endocytosis in Prominin2 expressing cells. -- Abstract: Background: Membrane protrusions play important roles in biological processes such as cell adhesion, wound healing, migration, and sensing of the external environment. Cell protrusions are a subtype of membrane microdomains composed of cholesterol and sphingolipids, and can be disrupted by cholesterol depletion. Prominins are pentaspan membrane proteins that bind cholesterol and localize to plasma membrane (PM) protrusions. Prominin-1 is of great interest as a marker for stem and cancer cells, while Prominin-2 (Prom2) is reportedly restricted to epithelial cells. Aim: To characterize the effects of Prom-2 expression on PM microdomain organization. Methods: Prom2-fluorescent protein was transfected in human skin fibroblasts (HSF) and Chinese hamster ovary (CHO) cells for PM raft and endocytic studies. Caveolae at PM were visualized using transmission electron microscopy. Cdc42 activation was measured and caveolin-1 knockdown was performed using siRNAs. Results: Prom2 expression in HSF and CHO cells caused extensive Prom2-positive protrusions that co-localized with lipid raft markers. Prom2 expression significantly decreased caveolae at the PM, reduced caveolar endocytosis and increased caveolin-1 phosphorylation. Prom2 expression also inhibited Cdc42-dependent fluid phase endocytosis via decreased Cdc42 activation. Effects on endocytosis were reversed by addition of cholesterol. Knockdown of caveolin-1 by siRNA restored Cdc42 dependent fluid phase endocytosis in Prom2-expressing cells. Conclusions: Prom2 protrusions primarily

  16. Rac1 and Cdc42 are regulators of HRasV12-transformation and angiogenic factors in human fibroblasts

    International Nuclear Information System (INIS)

    Appledorn, Daniel M; Dao, Kim-Hien T; O'Reilly, Sandra; Maher, Veronica M; McCormick, J Justin

    2010-01-01

    The activities of Rac1 and Cdc42 are essential for HRas-induced transformation of rodent fibroblasts. What is more, expression of constitutively activated mutants of Rac1 and/or Cdc42 is sufficient for their malignant transformation. The role for these two Rho GTPases in HRas-mediated transformation of human fibroblasts has not been studied. Here we evaluated the contribution of Rac1 and Cdc42 to maintaining HRas-induced transformation of human fibroblasts, and determined the ability of constitutively activated mutants of Rac1 or Cdc42 to induce malignant transformation of a human fibroblast cell strain. Under the control of a tetracycline regulatable promoter, dominant negative mutants of Rac1 and Cdc42 were expressed in a human HRas-transformed, tumor derived fibroblast cell line. These cells were used to determine the roles of Rac1 and/or Cdc42 proteins in maintaining HRas-induced transformed phenotypes. Similarly, constitutively active mutants were expressed in a non-transformed human fibroblast cell strain to evaluate their potential to induce malignant transformation. Affymetrix GeneChip arrays were used for transcriptome analyses, and observed expression differences were subsequently validated using protein assays. Expression of dominant negative Rac1 and/or Cdc42 significantly altered transformed phenotypes of HRas malignantly transformed human fibroblasts. In contrast, expression of constitutively active mutants of Rac1 or Cdc42 was not sufficient to induce malignant transformation. Microarray analysis revealed that the expression of 29 genes was dependent on Rac1 and Cdc42, many of which are known to play a role in cancer. The dependence of two such genes, uPA and VEGF was further validated in both normoxic and hypoxic conditions. The results presented here indicate that expression of both Rac1 and Cdc42 is necessary for maintaining several transformed phenotypes in oncogenic HRas transformed human cells, including their ability to form tumors in athymic

  17. Rac1 and Cdc42 are regulators of HRasV12-transformation and angiogenic factors in human fibroblasts

    Directory of Open Access Journals (Sweden)

    Dao Kim-Hien T

    2010-01-01

    Full Text Available Abstract Background The activities of Rac1 and Cdc42 are essential for HRas-induced transformation of rodent fibroblasts. What is more, expression of constitutively activated mutants of Rac1 and/or Cdc42 is sufficient for their malignant transformation. The role for these two Rho GTPases in HRas-mediated transformation of human fibroblasts has not been studied. Here we evaluated the contribution of Rac1 and Cdc42 to maintaining HRas-induced transformation of human fibroblasts, and determined the ability of constitutively activated mutants of Rac1 or Cdc42 to induce malignant transformation of a human fibroblast cell strain. Methods Under the control of a tetracycline regulatable promoter, dominant negative mutants of Rac1 and Cdc42 were expressed in a human HRas-transformed, tumor derived fibroblast cell line. These cells were used to determine the roles of Rac1 and/or Cdc42 proteins in maintaining HRas-induced transformed phenotypes. Similarly, constitutively active mutants were expressed in a non-transformed human fibroblast cell strain to evaluate their potential to induce malignant transformation. Affymetrix GeneChip arrays were used for transcriptome analyses, and observed expression differences were subsequently validated using protein assays. Results Expression of dominant negative Rac1 and/or Cdc42 significantly altered transformed phenotypes of HRas malignantly transformed human fibroblasts. In contrast, expression of constitutively active mutants of Rac1 or Cdc42 was not sufficient to induce malignant transformation. Microarray analysis revealed that the expression of 29 genes was dependent on Rac1 and Cdc42, many of which are known to play a role in cancer. The dependence of two such genes, uPA and VEGF was further validated in both normoxic and hypoxic conditions. Conclusion(s The results presented here indicate that expression of both Rac1 and Cdc42 is necessary for maintaining several transformed phenotypes in oncogenic HRas

  18. CDC42EP4, a perisynaptic scaffold protein in Bergmann glia, is required for glutamatergic tripartite synapse configuration.

    Science.gov (United States)

    Ageta-Ishihara, Natsumi; Konno, Kohtarou; Yamazaki, Maya; Abe, Manabu; Sakimura, Kenji; Watanabe, Masahiko; Kinoshita, Makoto

    2018-01-09

    Configuration of tripartite synapses, comprising the pre-, post-, and peri-synaptic components (axon terminal or bouton, dendritic spine, and astroglial terminal process), is a critical determinant of neurotransmitter kinetics and hence synaptic transmission. However, little is known about molecular basis for the regulation of tripartite synapse morphology. Previous studies showed that CDC42EP4, an effector protein of a cell morphogenesis regulator CDC42, is expressed exclusively in Bergmann glia in the cerebellar cortex, that it forms tight complex with the septin heterooligomer, and that it interacts indirectly with the glutamate transporter GLAST and MYH10/nonmuscle myosin ΙΙB. Scrutiny of Cdc42ep4 -/- mice had revealed that the CDC42EP4-septins-GLAST interaction facilitates glutamate clearance, while the role for CDC42EP4-septins-MYH10 interaction has remained unsolved. Here, we find anomalous configuration of the tripartite synapses comprising the parallel fiber boutons, dendritic spines of Purkinje cells, and Bergmann glial processes in Cdc42ep4 -/- mice. The complex anomalies include 1) recession of Bergmann glial membranes from the nearest active zones, and 2) extension of nonactive synaptic contact around active zone. In line with the recession of Bergmann glial membranes by the loss of CDC42EP4, overexpression of CDC42EP4 in heterologous cells promotes cell spreading and partitioning of MYH10 to insoluble (i.e., active) fraction. Paradoxically, however, Cdc42ep4 -/- cerebellum contained significantly more MYH10 and N-cadherin, which is attributed to secondary neuronal response mainly in Purkinje cells. Given cooperative actions of N-cadherin and MYH10 for adhesion between neurons, we speculate that their augmentation may reflect the extension of nonactive synaptic contacts in Cdc42ep4 -/- cerebellum. Transcellular mechanism that links the absence of CDC42EP4 in Bergmann glia to the augmentation of N-cadherin and MYH10 in neurons is currently unknown

  19. Impacto econômico no tratamento do glaucoma: volume de gotas de colírios antiglaucomatosos brasileiros e norte-americanos Economic impact of glaucoma treatment: Brazilian and North-american antiglaucomatous eyedrop volumes

    Directory of Open Access Journals (Sweden)

    Roberto Roizenblatt

    2001-04-01

    Full Text Available Objetivo: Comparar o exame de gotas de colírios antiglaucomatosos norte-americanos e brasileiros, e a repercussão no custo do tratamento. Métodos: Estudo volumétrico da gota e conseqüente número de gotas por mililitro, com estabelecimento da duração média de cada frasco de colírio para a posologia e custo anual do tratamento. Resultados: A gota de Alphagan® brasileiro foi em média 18% maior que a norte-americana, com duração média de 30,8 dias e custo anual de R$ 440,70; o AlphaganTM norte-americano teve duração média de 36,3 dias e custo anual de R$374,10, ou seja, 17,8% de diferença no custo anual. A gota de Betoptic S® brasileiro foi 38,4% maior que a norte-americana, com duração de 31,3 dias e custo anual de R$ 192,60; o Betoptic S TM norte-americano teve duração de 43,3 dias e custo anual de R$ 139,40, ou seja, 38,1% de diferença no custo anual. A gota de Iopidine® brasileiro foi 46,3% maior que a norte-americana, com duração de 35,7 dias e custo anual de R$ 365,00; o IopidineTM norte-americano teve duração de 52,6 dias e custo anual de R$ 248,40, ou seja, 46,9% de diferença no custo anual. A gota de Timoptol® foi 14,7% maior que a norte-americana, com duração de 45,4 dias e custo anual de R$ 58,40; o TimopticTM norte-americano teve duração de 52,1 dias e custo anual de R$ 51,00, ou seja, 14,5% de diferença no custo anual. Conclusões: Em todos os colírios o volume da gota brasileira foi estatisticamente maior, acarretando menor duração do frasco, maior custo anual do tratamento com desperdício significativo e prejuízo para o consumidor.Purpose: To assess the volume of each drop of Brazilian commercially available antiglaucomatous eyedrops and to compare the results with North-american equivalents in order to evaluate the economic implications. Methods: Volumetric study of drops and related impact in the cost of treatment. Results: Brazilian Alphagan® drop volume was 18% larger and the bottle had a

  20. Genetic deletion of cdc42 reveals a crucial role for astrocyte recruitment to the injury site in vitro and in vivo

    DEFF Research Database (Denmark)

    Robel, Stefanie; Bardehle, Sophia; Lepier, Alexandra

    2011-01-01

    signals, the small RhoGTPase Cdc42, selectively in mouse astrocytes in vitro and in vivo. We used an in vitro scratch assay as a minimal wounding model and found that astrocytes lacking Cdc42 (Cdc42Δ) were still able to form protrusions, although in a nonoriented way. Consequently, they failed to migrate...... in a directed manner toward the scratch. When animals were injured in vivo through a stab wound, Cdc42Δ astrocytes developed protrusions properly oriented toward the lesion, but the number of astrocytes recruited to the lesion site was significantly reduced. Surprisingly, however, lesions in Cdc42Δ animals...

  1. The establishment of injury surveillance systems in Colombia, El Salvador, and Nicaragua (2000-2006 Establecimiento de sistemas de vigilancia de lesiones en Colombia, El Salvador y Nicaragua (2000-2006

    Directory of Open Access Journals (Sweden)

    Benjamin A. Sklaver

    2008-12-01

    Full Text Available La Organización Panamericana de la Salud (OPS, en colaboración con los Centros para el Control y la Prevención de Enfermedades (CDC de los Estados Unidos de América, estableció en 2000 sistemas para la vigilancia de lesiones en Colombia, El Salvador y Nicaragua. Estos sistemas, basados en los servicios hospitalarios de emergencia, formaron parte de un proyecto piloto fundamentado en las guías para la vigilancia de lesiones, desarrolladas por la Organización Mundial de la Salud (OMS y los CDC. Los objetivos de este proyecto eran evaluar la utilidad de las guías de la OMS/CDC, crear mecanismos apropiados para la vigilancia de lesiones en los tres países, promover intervenciones preventivas adecuadas e integrar la prevención y el control de lesiones en proyectos nacionales de salud pública. En este artículo se describe el proyecto piloto de la OPS/CDC y se resaltan los principios más importantes y las lecciones aprendidas durante sus seis años de funcionamiento (2000-2006. Entre las principales recomendaciones se encuentran la integración de la vigilancia de las lesiones en las unidades de epidemiología e información existentes en los ministerios de salud, la recogida de los datos importantes exclusivamente, la garantía de monitoreos y evaluaciones adecuados y la adopción de las prácticas que han demostrado ser más efectivas para el entrenamiento de personal y la diseminación de información.

  2. Sistemas territoriales de salud frente al sistema general de seguridad social en salud de Colombia

    Directory of Open Access Journals (Sweden)

    Jahir Alexander Gutiérrez Ossa

    2014-06-01

    Full Text Available Introducción: El artículo expone los elementos y fundamentos que podrían avalar la generación de sistemas de seguridad social en salud territorial, en complementación con el sistema general preexistente. Materiales y Métodos: La eclosión generalizada en los sistemas de salud, implica la revisión de los modelos propuestos de carácter central y territorial. El análisis económico institucional, permite analizar las condiciones que tienen los sistemas generales para impactar en los esquemas territoriales. Resultados: Es el momento preciso, para que el sistema general en Colombia responda efectivamente en la materia, y que tanto ello, podría dar cuenta de la capacidad sistémica del mismo en el escenario territorial. Discusión: Es importante, que las gobernaciones y municipios planteen el alcance de dicha iniciativa. El Sistema General de Seguridad Social en Salud en Colombia, gira en torno a modelos y no a la concepción de sistema de salud efectivamente. Conclusiones: La definición de sistema de salud está opacando la atención para definir lo que implica efectivamente la salud, independiente de cualquier sistema al que se pretenda apuntar al final. Palabras clave: Cobertura de Servicios Públicos de Salud, Evaluación en Salud, Sistemas Multiinstitucionales, Sistemas Nacionales de Salud, Políticas Públicas de Salud. (Fuente: DeCS BIREME. Cómo citar este artículo: Gutiérrez Ossa JA, Restrepo Avendaño RD. Sistemas territoriales de salud frente al sistema general de seguridad social en salud de Colombia. Rev Cuid. 2014; 5(1: 623-32.

  3. Cdc42-dependent leading edge coordination is essential for interstitial dendritic cell migration

    DEFF Research Database (Denmark)

    Lammermann, Tim; Renkawitz, Jorg; Wu, Xunwei

    2009-01-01

    proteolysis. Instead, the protrusive flow of the actin cytoskeleton directly drives a basal mode of locomotion that is occasionally supported by actomyosin contractions at the trailing edge to propel the cell's rigid nucleus. We here delete the small GTPase Cdc42 in DCs and find that actin flow and actomyosin......Mature dendritic cells (DCs) moving from the skin to the lymph node are a prototypic example of rapidly migrating amoeboid leukocytes. Interstitial DC migration is directionally guided by chemokines, but independent of specific adhesive interactions with the tissue as well as pericellular...... contraction are still initiated in response to chemotactic cues. Accordingly, the cells are able to polarize and form protrusions. However, in the absence of Cdc42 the protrusions are temporally and spatially dysregulated which leads to impaired leading edge coordination. While this defect still allows...

  4. Computer program CDCID: an automated quality control program using CDC update

    International Nuclear Information System (INIS)

    Singer, G.L.; Aguilar, F.

    1984-04-01

    A computer program, CDCID, has been developed in coordination with a quality control program to provide a highly automated method of documenting changes to computer codes at EG and G Idaho, Inc. The method uses the standard CDC UPDATE program in such a manner that updates and their associated documentation are easily made and retrieved in various formats. The method allows each card image of a source program to point to the document which describes it, who created the card, and when it was created. The method described is applicable to the quality control of computer programs in general. The computer program described is executable only on CDC computing systems, but the program could be modified and applied to any computing system with an adequate updating program

  5. LTSS compendium: an introduction to the CDC 7600 and the Livermore Timesharing System

    International Nuclear Information System (INIS)

    Fong, K.W.

    1977-01-01

    This report is an introduction to the CDC 7600 computer and to the Livermore Timesharing System (LTSS) used by the National Magnetic Fusion Energy Computer Center (NMFECC) and the Lawrence Livermore Laboratory Computer Center (LLLCC or Octopus network) on their 7600's. This report is based on a document originally written specifically about the system as it is implemented at NMFECC but has been broadened to point out differences in implementation at LLLCC. It also contains information about LLLCC not relevant to NMFECC. This report is written for computational physicists who want to prepare large production codes to run under LTSS on the 7600's. The generalized discussion of the operating system focuses on creating and executing controllees. This document and its companion, UCID-17557, CDC 7600 LTSS Programming Stratagems, provide a basis for understanding more specialized documents about individual parts of the system

  6. Educação bilíngüe e multiculturalismo: o exemplo americano = Bilingual education and multiculturalism: the american example

    Directory of Open Access Journals (Sweden)

    Naiditch, Fernando

    2007-01-01

    Full Text Available Este artigo apresenta uma visão histórica de educação bilingüe nos Estados Unidos e mostra que a forma como ela foi compreendida mudou através dos anos. O artigo focaliza as diferentes políticas educacionais e a legislação que regulamentam o uso de outras linguas que não o inglês no sistema educacional público e privado nos EUA. Políticas públicas que tratam do ensino de inglês como segunda língua também são discutidas. A perspectiva histórica apresentada no artigo percorre diferentes momentos da história americana e mostra como eles afetaram as políticas educacionais que guiam o uso do inglês e de linguas estrangeiras nas escolas (ex: imigração, o Sputnik, o movimento de direitos civis, e mais recentemente o Nenhuma Criança Deixada para Trás. O artigo mostra as vantagens de se viver em uma sociedade multicultural, e também como o sistema de educação pública pode se beneficiar deste fato, e como administradores e educadores devem abordar diversidade lingüística e cultural tanto dentro como fora da sala de aula

  7. CDC Vital Signs–Native Americans With Diabetes

    Centers for Disease Control (CDC) Podcasts

    2017-01-10

    This podcast is based on the January 2017 CDC Vital Signs report. Diabetes is the leading cause of kidney failure and Native Americans have a greater chance of having diabetes than any other racial group in the U.S. Learn how to manage your diabetes to delay or prevent kidney failure.  Created: 1/10/2017 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 1/10/2017.

  8. CDC Vital Signs–HIV and Injection Drug Use

    Centers for Disease Control (CDC) Podcasts

    2016-11-29

    This podcast is based on the December 2016 CDC Vital Signs report. Sharing needles, syringes, and other injection equipment puts you at risk for getting HIV and other infections, including hepatitis. Learn how to reduce your HIV risk.  Created: 11/29/2016 by National Center for HIV/AIDS, Viral Hepatitis, Sexual Transmitted Diseases and Tuberculosis Prevention (NCHHSTP).   Date Released: 11/29/2016.

  9. CDC Vital Signs–Dental Sealants Prevent Cavities

    Centers for Disease Control (CDC) Podcasts

    2016-10-18

    This podcast is based on the October 2016 CDC Vital Signs report. Dental sealants, applied soon after a child's permanent molars come in, can protect against cavities for up to nine years. Applying sealants in schools for low-income children could save millions in dental treatment costs.  Created: 10/18/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/18/2016.

  10. CDC Signos Vitales: Piense en la septicemia. El tiempo es crucial. (Think Sepsis. Time Matters.)

    Centers for Disease Control (CDC) Podcasts

    2016-08-23

    Este podcast se basa en la edición de agosto del 2016 del informe Signos Vitales de los CDC. La septicemia es una emergencia médica y puede ocurrir rápidamente. Conozca los signos de la septicemia y la forma de prevenirla.  Created: 8/23/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 8/23/2016.

  11. CDC Vital Signs–Motor Vehicle Crash Deaths

    Centers for Disease Control (CDC) Podcasts

    2016-07-06

    This podcast is based on the July 2016 CDC Vital Signs report. In the U.S., about 90 people die in motor vehicle crashes each day and thousands more are injured, resulting in hundreds of millions of dollars in direct medical costs each year. Learn what you can do to stay safe.  Created: 7/6/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 7/6/2016.

  12. NEK11 regulates CDC25A degradation and the IR-induced G2/M checkpoint

    DEFF Research Database (Denmark)

    Melixetian, M.; Helin, K.; Klein, D.K.

    2009-01-01

    new genes involved in the G2/M checkpoint we performed a large-scale short hairpin RNA (shRNA) library screen. We show that NIMA (never in mitosis gene A)-related kinase 11 (NEK11) is required for DNA damage-induced G2/M arrest. Depletion of NEK11 prevents proteasome-dependent degradation of CDC25A...... that genetic mutations in NEK11 may contribute to the development of human cancer....

  13. CDC Vital Signs-Zika and Pregnancy: What You Should Know

    Centers for Disease Control (CDC) Podcasts

    2016-04-01

    This podcast is based on the April 2016 CDC Vital Signs report. A pregnant woman who is infected with Zika virus can pass it to her fetus which is linked to microcephaly, a serious birth defect. This podcast discusses how to protect yourself from Zika virus.  Created: 4/1/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 4/1/2016.

  14. CDC28, NETI, and HFII are required for checkpoints in Saccharomyces cerevisiae

    International Nuclear Information System (INIS)

    Koltovaya, N.A.; Kadyshevskaya, E.Yu.; Roshina, M.P.; Devin, A.B.

    2009-01-01

    The involvement of SRM genes selected as genes affecting genetic stability and radiosensitivity in a cell cycle arrest under the action of damaging agents was studied. It was shown that the srm5/cdc28-srm, srm8/netI-srm, and srmI2/hfiI-srm mutations prevent checkpoint activation by DNA damage, particularly the G 0 /S (srm5, srm8), G 1 /S (srm5, srm8, srm12), S (srm8, srm12) and S/G 2 (srm5) checkpoints. It seems that in budding yeast the CDC28, HFII/ADAI, and NETI genes mediate cellular response induced by DNA damage with checkpoint control. The well-known checkpoint-genes RAD9, RAD17, RAD24, and RAD53, and the genes CDC28, and NETI have been found to belong to one epistasis group named RAD9-group as regards cell sensitivity to γ radiation. An analysis of the radiosensitivity of double mutants has revealed that the mutation cdc-28-srm is hypostatic to each of mutations rad9Δ, and rad24Δ, and additive to rad17Δ. The mutation netI-srm is hypostatic to the mutations rad9Δ but additive to rad17Δ, rad24Δ, and rad53. The mutation hfiI-srm has an additive effect in compound with the mutations rad24Δ and rad9Δ. So, investigations of epistatic interactions have demonstrated a branched RAD9-dependent pathway. The analyzed genes can also participate in a minor mechanism involved in determining cell radiation sensitivity independently of the mentioned RAD9-dependent pathway

  15. CDC Vital Signs–Too Loud for Too Long!

    Centers for Disease Control (CDC) Podcasts

    2017-02-07

    This podcast is based on the February 2017 CDC Vital Signs report. Being around too much loud noise—like a leaf blower or rock concert—can cause permanent hearing loss. Learn how to prevent hearing loss.  Created: 2/7/2017 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 2/7/2017.

  16. Prevention and control of tuberculosis in correctional and detention facilities: recommendations from the CDC

    CSIR Research Space (South Africa)

    Parsons, S

    2006-07-01

    Full Text Available roles of shared responsibility. The recommendations in this report can assist officials of federal, state, and local correctional facilities in preventing trans- mission of TB and controlling TB among inmates and facility employees. The target...-care professionals. The report is intended to assist policymakers in reaching informed decisions regarding the pre- vention and control of TB in correctional facilities. Methods To update the existing guidelines, with assistance from ACET, CDC organized...

  17. Semantic representation of CDC-PHIN vocabulary using Simple Knowledge Organization System.

    Science.gov (United States)

    Zhu, Min; Mirhaji, Parsa

    2008-11-06

    PHIN Vocabulary Access and Distribution System (VADS) promotes the use of standards based vocabulary within CDC information systems. However, the current PHIN vocabulary representation hinders its wide adoption. Simple Knowledge Organization System (SKOS) is a W3C draft specification to support the formal representation of Knowledge Organization Systems (KOS) within the framework of the Semantic Web. We present a method of adopting SKOS to represent PHIN vocabulary in order to enable automated information sharing and integration.

  18. CDC Vital Signs-ADHD in Young Children: What You Should Know

    Centers for Disease Control (CDC) Podcasts

    2016-05-03

    This podcast is based on the May 2016 CDC Vital Signs report. For children ages two to five who have ADHD, behavior therapy is recommended before prescribing medicine. This therapy teaches parents ways to improve their child’s behavior and can work as well as medicine, without the risk of side effects.  Created: 5/3/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 5/3/2016.

  19. Distribuição da altura uterina ao longo da gestação em uma coorte brasileira: comparação com a curva de referência do Centro Latino-Americano de Perinatologia Distribution of uterine height during pregnancy in a Brazilian cohort: comparison with the reference curve of the Centro Latino-Americano de Perinatologia

    Directory of Open Access Journals (Sweden)

    Maria Lúcia Rocha Oppermann

    2006-09-01

    Full Text Available OBJETIVOS: descrever, em gestantes do Estudo Brasileiro do Diabetes Gestacional (EBDG, a distribuição da altura uterina de acordo com a idade gestacional e validar a curva do Centro Latino-Americano de Perinatologia (CLAP, curva de referência para a predição de anormalidades do crescimento fetal. MÉTODOS: o EBDG é uma coorte de 5564 gestantes, com mais de 19 anos, seguidas até e após o parto. Entrevistas e medidas antropométricas padronizadas foram feitas no arrolamento entre a 20ª e a 28ª semana. Os prontuários foram revisados segundo protocolo padronizado, abrangendo os períodos de pré-natal e parto. As análises referem-se a 3539 gestantes com datação da gravidez confirmada por ultra-sonografia. Determinamos as propriedades diagnósticas dos percentis 10 e 90 de altura uterina de ambas as curvas (EBDG e CLAP como indicadores de anormalidade no peso neonatal. RESULTADOS: as medidas de altura uterina no EBDG foram maiores que as do CLAP em todas as semanas de gestação (1 a 4 cm e 2 a 6 cm, respectivamente, nos percentis 10 e 90. O percentil 10 do CLAP identificou como pequenas as medidas uterinas de 0,3 a 1,7% das gestantes brasileiras, ao passo que o percentil 90 classificou como grandes as medidas uterinas de 42 a 57% das brasileiras. A sensibilidade do percentil 10 do CLAP para predizer recém-nascidos pequenos para a idade gestacional variou de 0,8 a 6% e a especificidade do percentil 90 para predizer grandes para a idade gestacional, de 46 a 61%. CONCLUSÕES: a curva de referência do CLAP não reflete o padrão de crescimento uterino das gestantes brasileiras, limitando sua capacidade de identificar anormalidades de crescimento fetal, especialmente a restrição de crescimento.PURPOSE: to describe, in participants of the Brazilian Study of Gestational Diabetes (EBDG, the percentile distribution of uterine height by gestational age and to validate the use of percentiles of the chart derived by the "Centro Latino-Americano

  20. Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.

    Science.gov (United States)

    Li, Ran; Yuan, Fengjie; Fu, Wan; Zhang, Luyao; Zhang, Nan; Wang, Yanan; Ma, Ke; Li, Xue; Wang, Lina; Zhu, Wei-Guo; Zhao, Ying

    2017-02-17

    The serine/threonine kinase Unc-51-like kinase-1 (Ulk1) is thought to be essential for induction of autophagy, an intracellular bulk degradation process that is activated by various stresses. Although several proteins have been suggested as Ulk1 substrates during autophagic process, it still remains largely unknown about Ulk1's physiological substrates. Here, by performing in vitro and in vivo phosphorylation assay, we report that the co-chaperone cell division cycle protein 37 (Cdc37) is a Ulk1 substrate. Ulk1-mediated phosphorylation of Ser-339 in Cdc37 compromised the recruitment of client kinases to a complex comprising Cdc37 and heat shock protein 90 (Hsp90) but only modestly affected Cdc37 binding to Hsp90. Because the recruitment of protein kinase clients to the Hsp90 complex is essential for their stability and functions, Ser-339 phosphorylation of Cdc37 disrupts its ability as a co-chaperone to coordinate Hsp90. Hsp90 inhibitors are cancer chemotherapeutic agents by inducing depletion of clients, many of which are oncogenes. Upon treatment with an Hsp90 inhibitor in cancer cells, Ulk1 promoted the degradation of Hsp90-Cdc37 client kinases, resulting in increased cellular sensitivity to Hsp90 inhibitors. Thus, our study provides evidence for an anti-proliferative role of Ulk1 in response to Hsp90 inhibition in cancer cells. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  1. PUBG; purex solvent extraction process model. [IBM3033; CDC CYBER175; FORTRAN IV

    Energy Technology Data Exchange (ETDEWEB)

    Geldard, J.F.; Beyerlein, A.L.

    PUBG is a chemical model of the Purex solvent extraction system, by which plutonium and uranium are recovered from spent nuclear fuel rods. The system comprises a number of mixer-settler banks. This discrete stage structure is the basis of the algorithms used in PUBG. The stages are connected to provide for countercurrent flow of the aqueous and organic phases. PUBG uses the common convention that has the aqueous phase enter at the lowest numbered stage and exit at the highest one; the organic phase flows oppositely. The volumes of the mixers are smaller than those of the settlers. The mixers generate a fine dispersion of one phase in the other. The high interfacial area is intended to provide for rapid mass transfer of the plutonium and uranium from one phase to the other. The separation of this dispersion back into the two phases occurs in the settlers. The species considered by PUBG are Hydrogen (1+), Plutonium (4+), Uranyl Oxide (2+), Plutonium (3+), Nitrate Anion, and reductant in the aqueous phase and Hydrogen (1+), Uranyl Oxide (2+), Plutonium (4+), and TBP (tri-n-butylphosphate) in the organic phase. The reductant used in the Purex process is either Uranium (4+) or HAN (hydroxylamine nitrate).IBM3033;CDC CYBER175; FORTRAN IV; OS/MVS or OS/MVT (IBM3033), NOS 1.3 (CDC CYBER175); The IBM3033 version requires 150K bytes of memory for execution; 62,000 (octal) words are required by the CDC CYBER175 version..

  2. Inhibition of Cdc42 and Rac1 activities in pheochromocytoma, the adrenal medulla tumor.

    Science.gov (United States)

    Croisé, Pauline; Brunaud, Laurent; Tóth, Petra; Gasman, Stéphane; Ory, Stéphane

    2017-04-03

    Altered Rho GTPase signaling has been linked to many types of cancer. As many small G proteins, Rho GTPases cycle between an active and inactive state thanks to specific regulators that catalyze exchange of GDP into GTP (Rho-GEF) or hydrolysis of GTP into GDP (Rho-GAP). Recent studies have shown that alteration takes place either at the level of Rho proteins themselves (expression levels, point mutations) or at the level of their regulators, mostly RhoGEFs and RhoGAPs. Most reports describe Rho GTPases gain of function that may participate to the tumorigenesis processes. In contrast, we have recently reported that decreased activities of Cdc42 and Rac1 as well as decreased expression of 2 Rho-GEFs, FARP1 and ARHGEF1, correlate with pheochromocytomas, a tumor developing in the medulla of the adrenal gland (Croisé et al., Endocrine Related Cancer, 2016). Here we highlight the major evidence and further study the correlation between Rho GTPases activities and expression levels of ARHGEF1 and FARP1. Finally we also discuss how the decrease of Cdc42 and Rac1 activities may help human pheochromocytomas to develop and comment the possible relationship between FARP1, ARHGEF1 and the 2 Rho GTPases Cdc42 and Rac1 in tumorigenesis.

  3. Vectorization of transport and diffusion computations on the CDC Cyber 205

    International Nuclear Information System (INIS)

    Abu-Shumays, I.K.

    1985-01-01

    This work focuses on development and testing of alternative numerical methods and computational algorithms specifically designed for the vectorization of transport and diffusion computations on a Control Data Corporation (CDC) Cyber 205 vector computer. The first part of the report summarizes and compares two solution methods for the discrete ordinates approximation to the transport equation. Factors of 4 to 7 reduction in run times for certain large transport problems were achieved on a Cyber 205 as compared with run times on a CDC-7600. The second part of the paper deals with the solution of tridiagonal systems of linear equations which is a central part for several efficient numerical methods for multidimensional diffusion computations and which is also essential for fluid flow and other physics and engineering problems. Among the methods tested, a combined odd-even cyclic reduction and modified Cholesky factorization algorithm for solving linear symmetric positive definite tridiagonal systems is found to be the most effective for these systems on a Cyber 205. For large tridiagonal systems, computation with this algorithm is an order of magnitude faster on a Cyber 205 than computation with the best algorithm for tridiagonal systems on a CDC-7600

  4. Contexto General de los Sistemas Agrarios

    Directory of Open Access Journals (Sweden)

    Vélez Escobar Norberto

    1989-12-01

    Full Text Available Esta ponencia introdujo y delimitó el campo de trabajo para el panel: Formas o Sistemas de Producción Agraria. En ella se presentó y discutió la definición del concepto de FORMA DE PRODUCCION e igualmente planteó la taxonomía más general o "agrupamientos" de los sistemas de producción agraria en Colombia. Se identificaron cinco grupos de formas, en proceso de estructuración, empresariales, campesinas, de economía natural y experimentales; y a continuación se caracterizó y explicó cada uno de los cinco agrupamientos. La ponencia terminó sustentando la validez del trabajo académico basado en el enfoque de sistemas y presentó la evolución reciente de esta forma de trabajo en la Universidad Nacional, Seccional Medellín.

  5. SCODA para el Desarrollo de Sistemas Multiagente

    Directory of Open Access Journals (Sweden)

    Jesús A. Román

    2011-12-01

    Full Text Available El planteamiento de implementar sistemas multiagente de forma que puedan reutilizarse con distintos objetivos, dota a los desarrolladores de una metodología más eficaz en sus fines. Este artículo presenta SCODA, una arquitectura basada en cinco principios: estandaridad, especialización, facilidad de implementación, reutilización y computación distribuida. SCODA pretende facilitar el desarrollo de sistemas multiagente, basándose en el concepto de pequeños grupos de agentes denominados Comunidades Inteligentes Especializadas (CIE. Las CIE cuentan con funcionalidades específicas que permiten llevar a cabo la implementación de sistemas multiagente de forma escalable, dentro del marco de SCODA, pudiendo ser reutilizadas en diversos desarrollos.

  6. Co-expression of CDC2 and CDK4 in human cancer cells is disrupted following γ-irradiation

    International Nuclear Information System (INIS)

    Warenius, Hilmar; Seabra, Laurence

    1995-01-01

    Purpose/Objective: Radiation delays progress through both the G1/S and G2/M checkpoints and also causes proliferation-related death in eukaryotic cells. The transition from G2 to mitosis is known to be controlled by the p 34 cdc2/cyclin B complex. Progress from G1 to S-phase is less well understood but is believed to involve, in part, the formation of a quaternary complex of PCNA, p21, cyclin D and cdk4. Here we examine putative relationships between the cdc2 and cdc4 cyclin-dependent kinases and exposure to γ-irradiation. Materials and Methods: Expression of the cdc2, cdk4, cyclin B and cyclin D genes was measured by Western blotting in 20 human in vitro cancer cell lines covering a wide range of histology and intrinsic cellular radiosensitivity. In order to examine whether the relationship between cdc2 and cdk4 expression was influenced by radiation we chose seven (HX142, 1407, 2780, MGHU, RT112, OAW42 and HRT18) of the human in vitro cell lines which we have previously shown to cover a wide range of intrinsic cellular radiosensitivities and which also showed a correlation between cdc2 and cdk4 expression of similar strength to the full twenty cell lines. The CDK protein levels were then followed by Western blots at 1, 24 and 48 hours after 2Gy of γ-irradiation from a 137 Cs unit. Results: Whilst no obvious relationship was apparent between the expression of cyclin B and cyclin D in the 20 cell lines, cdc2 and cdk4 appeared to be expressed to similar degrees in each cell line. Quantitation of cdc2 and cdk4 proteins by photodensitometry confirmed that there was a strong correlation (r=0.87, p=0.000006) between the two CDKs though only a weak relationship (r=0.491), between the respective cyclins with which cdc2 and cdk4 are known to complex in normal cells. We also compared protein levels of 10 proliferation-related gene produces including cdc2, cdk4 and cyclins B and D. Only cdc2 and cdk4 expression was completely disrupted at 1 hour. Sham irradiated cell lines

  7. Mesenchymal-epithelial transition during somitic segmentation is regulated by differential roles of Cdc42 and Rac1.

    Science.gov (United States)

    Nakaya, Yukiko; Kuroda, Shinya; Katagiri, Yuji T; Kaibuchi, Kozo; Takahashi, Yoshiko

    2004-09-01

    Mesenchymal-epithelial transitions (MET) are crucial for vertebrate organogenesis. The roles of Rho family GTPases in such processes during actual development remain largely unknown. By electroporating genes into chick presomitic mesenchymal cells, we demonstrate that Cdc42 and Rac1 play important and different roles in the MET that generates the vertebrate somites. Presomitic mesenchymal cells, which normally contribute to both the epithelial and mesenchymal populations of the somite, were hyperepithelialized when Cdc42 signaling was blocked. Conversely, cells taking up genes that elevate Cdc42 levels remained mesenchymal. Thus, Cdc42 activity levels appear critical for the binary decision that defines the epithelial and mesenchymal somitic compartments. Proper levels of Rac1 are necessary for somitic epithelialization, since cells with activated or inhibited Rac1 failed to undergo correct epithelialization. Furthermore, Rac1 appears to be required for Paraxis to act as an epithelialization-promoting transcription factor during somitogenesis.

  8. Psiconeuroinmunología: Conexiones entre sistema nervioso y sistema inmune

    Directory of Open Access Journals (Sweden)

    García López, Luis Joaquín

    2008-09-01

    Full Text Available La psiconeuroinmunología es una nueva parcela de la ciencia que apenas lleva tres décadas, pero que en los últimos años ha sido objeto de una enorme atención por diversos grupos de investigación. El articulo pretende exponer brevemente cómo están organizados los sistemas inmune y neuroendocrino, para después presentar los hallazgos que hasta el momento intentan dar explicación de las relaciones entre ambos con el sistema nervioso, así como las influencias de unos sobre otros, más concretamente expresar la relación entre los tres sistemas responsables de la defensa del organismo y, más ampliamente, de su mantenimiento homeostático. Cuando se trata de amenazas, el sistema nervioso central tiene que decidir qué sistema se pone en marcha, el sistema de estrés como energía para el ataque o la huida (un animal que nos quiere atacar, el sistema inmune para neutralizar el antígeno (un virus que nos quiere atacar o ambos (la energía es necesaria también para el sistema inmune. Como parece ser que en muchas ocasiones la depresión aparece tras los esfuerzos infructuosos de puesta en marcha de estrategias de afrontamiento, también hemos repasado sucintamente algunas investigaciones que relacionan al sistema inmune con la depresión, así como con los trastornos del sueño. Se finaliza con las aportaciones que la psicología puede ofrecer a nivel terapéutico.

  9. Saccharomyces cerevisiae Cdc42p GTPase Is Involved in Preventing the Recurrence of Bud Emergence during the Cell Cycle

    Science.gov (United States)

    Richman, Tamara J.; Johnson, Douglas I.

    2000-01-01

    The Saccharomyces cerevisiae Cdc42p GTPase interacts with multiple regulators and downstream effectors through an ∼25-amino-acid effector domain. Four effector domain mutations, Y32K, F37A, D38E, and Y40C, were introduced into Cdc42p and characterized for their effects on these interactions. Each mutant protein showed differential interactions with a number of downstream effectors and regulators and various levels of functionality. Specifically, Cdc42D38Ep showed reduced interactions with the Cla4p p21-activated protein kinase and the Bem3p GTPase-activating protein and cdc42D38E was the only mutant allele able to complement the Δcdc42 null mutant. However, the mutant protein was only partially functional, as indicated by a temperature-dependent multibudded phenotype seen in conjunction with defects in both septin ring localization and activation of the Swe1p-dependent morphogenetic checkpoint. Further analysis of this mutant suggested that the multiple buds emerged consecutively with a premature termination of bud enlargement preceding the appearance of the next bud. Cortical actin, the septin ring, Cla4p-green fluorescent protein (GFP), and GFP-Cdc24p all predominantly localized to one bud at a time per multibudded cell. These data suggest that Cdc42D38Ep triggers a morphogenetic defect post-bud emergence, leading to cessation of bud growth and reorganization of the budding machinery to another random budding site, indicating that Cdc42p is involved in prevention of the initiation of supernumerary buds during the cell cycle. PMID:11046150

  10. The PP2AB56 phosphatase promotes the association of Cdc20 with APC/C in mitosis.

    Science.gov (United States)

    Lee, Sun Joo; Rodriguez-Bravo, Veronica; Kim, Hyunjung; Datta, Sutirtha; Foley, Emily A

    2017-05-15

    PP2A comprising B56 regulatory subunit isoforms (PP2A B56 ) is a serine/threonine phosphatase essential for mitosis. At the kinetochore, PP2A B56 both stabilizes microtubule binding and promotes silencing of the spindle assembly checkpoint (SAC) through its association with the SAC protein BubR1. Cells depleted of the B56 regulatory subunits of PP2A are delayed in activation of Cdc20-containing APC/C (APC/C Cdc20 ), which is an essential step for mitotic exit. It has been hypothesized that this delay arises from increased production of the mitotic checkpoint complex (MCC), an APC/C Cdc20 inhibitor formed at unattached kinetochores through SAC signaling. In contrast to this prediction, we show that depletion of B56 subunits does not increase the amount or stability of the MCC. Rather, delays in APC/C Cdc20 activation in B56-depleted cells correlate with impaired Cdc20 binding to APC/C. Stimulation of APC/C Cdc20 assembly does not require binding between PP2A B56 and BubR1, and thus this contribution of PP2A B56 towards mitotic exit is distinct from its functions at kinetochores. PP2A B56 associates with APC/C constitutively in a BubR1-independent manner. A mitotic phosphorylation site on Cdc20, known to be a substrate of PP2A B56 , modulates APC/C Cdc20 assembly. These results elucidate the contributions of PP2A B56 towards completion of mitosis. © 2017. Published by The Company of Biologists Ltd.

  11. Sistema de realidad aumentada para aplicaciones Android

    OpenAIRE

    Fernández Sánchez, Natalia Mercedes

    2012-01-01

    El objetivo principal de este proyecto es la creación de un sistema de realidad aumentada para el sistema operativo Android con idea de que sea utilizado para la creación de un videojuego, pero que a su vez sea lo suficientemente versátil como para poder utilizarse en otro tipo de aplicaciones. De este objetivo principal podemos obtener los objetivos secundarios: - Conocer las características que ofrecen los teléfonos móviles y como éstas pueden ser aprovechadas para la crea...

  12. CATEGORIZANDO A LOS USUARIOS DE SISTEMAS DIGITALES.

    OpenAIRE

    Denise Hernández y Hernández; Alberto Ramírez-Martinell; Daniel Cassany

    2014-01-01

    Repasamos las categorías más utilizadas para aludir a las características de las personas que utilizan algún tipo de sistema digital, ya sea por su grado de participación en la comu- nicación global y en los sistemas web -1.0 o 2.0-; por la actitud que toman ante el contenido digital -consumidores o productores-; por su intermitencia en el mundo digital -residentes o visitantes-; o por el momento histórico en el que nacieron -nativos digitales o inmigrantes digitales-, con el propósito de ref...

  13. Sistema de comunicaciones para una planta fotovoltaica

    OpenAIRE

    Martín Aragón, José Manuel

    2012-01-01

    Las plantas solares fotovoltaicas, que son cada vez más habituales en nuestra sociedad, necesitan contar con un sistema de comunicaciones que permita la monitorización continua del funcionamiento de los diferentes equipos así como el control remoto de los mismos y la regulación de la producción. En este Proyecto se ha estudiado la estructura eléctrica y constructiva de una planta fotovoltaica genérica, prestando especial atención a los requerimientos que debe reunir el sistema de comunicac...

  14. El Derecho Administrativo del Sistema Comunitario Andino

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Santos Rodríguez

    2013-12-01

    Full Text Available Una de las tendencias contemporáneas del Derecho Administrativo es el reconocimiento de su existencia más allá de las fronteras de un Estado. Bajo esa premisa, se busca demostrar que en la Comunidad Andina de Naciones se configuran los elementos suficientes para afirmar que existe un Derecho Administrativo Comunitario Andino, en la medida en que de las normas andinas se desprende la existencia de una función administrativa andina, una organización administrativa al seno del Sistema Andino de Integración, un sistema de fuentes administrativas andina y un contencioso administrativo comunitario.

  15. Desarrollo de un sistema de reconocimiento facial

    OpenAIRE

    Vivas Imparato, Abdón Alejandro

    2014-01-01

    El objetivo principal alrededor del cual se desenvuelve este proyecto es el desarrollo de un sistema de reconocimiento facial. Entre sus objetivos específicos se encuentran: realizar una primera aproximación sobre las técnicas de reconocimiento facial existentes en la actualidad, elegir una aplicación donde pueda ser útil el reconocimiento facial, diseñar y desarrollar un programa en MATLAB que lleve a cabo la función de reconocimiento facial, y evaluar el funcionamiento del sistema desarroll...

  16. Sistema de control remot per a hivernacles

    OpenAIRE

    Clar Ayarte, Tomàs

    2014-01-01

    En aquest projecte es configura un sistema encastat que interactua amb un conjunt de perifèrics per tal de proporcionar informació de l'entorn d'un hivernacle i connectivitat amb l'usuari a través d'un portal web. En este proyecto se configura un sistema embebido que interactúa con un conjunto de periféricos para proporcionar información del entorno de un invernadero y conectividad con el usuario a través de un portal web. Bachelor thesis for the Telecommunication Technologies program o...

  17. Sistema local de salud de Manizales

    OpenAIRE

    Gallego Mejía, Jesús Bernardo; Médico Especialista en Gerencia Hospitalaria U. de Antioquia. Docente Universidad de Manizales - Salud Pública Facultad de Medicina

    2006-01-01

    La salud es un resultado de las acciones tomadas en diferentes sectores; el sistema de servicios de salud deben conservar (con actividades de promoción y prevención) y recuperar la salud (con el tratamiento y nrehabilitación). A estos efectos, el sistema debe desarrollar estrategias eficaces para una individual, así como para grupos de personas de todos los niveles. Para una persona existe el PHO (Plan Obligatorio de Salud), y para los grupos hay PAB (Plan de Atención Básica), y hay son tambi...

  18. Sistema de gestión documental

    OpenAIRE

    Fernández Gómez, Javier

    2015-01-01

    Esta memoria expone la planificación, el análisis, el diseño y el desarrollo de un sistema de gestión documental, en adelante SGD, configurable, es decir, adaptable a un ámbito determinado. El SGD consta de dos grupos de interfaces o funcionalidades claramente diferenciadas: administración y gestión documental. Aquesta memòria exposa la planificació, l'anàlisi, el disseny i el desenvolupament d'un sistema de gestió documental, d'ara endavant SGA, configurable, és a dir, adaptable a un àmbi...

  19. Sistema de Control i Seguiment de Projectes (SCSP)

    OpenAIRE

    Virgili Llop, Joaquim

    2012-01-01

    Sistema de control i seguiment dels horaris dels consultors i les despeses associades a projectes de consultoria. Sistema de control y seguimiento de los horarios de los consultores y los gastos asociados a proyectos de consultoría.

  20. SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.

    Science.gov (United States)

    Singh, N Sadananda; Shao, Nan; McLean, Janel R; Sevugan, Mayalagu; Ren, Liping; Chew, Ting Gang; Bimbo, Andrea; Sharma, Reetu; Tang, Xie; Gould, Kathleen L; Balasubramanian, Mohan K

    2011-12-06

    Cytokinesis in many eukaryotes involves the function of an actomyosin-based contractile ring. In fission yeast, actomyosin ring maturation and stability require a conserved signaling pathway termed the SIN (septation initiation network). The SIN consists of a GTPase (Spg1p) and three protein kinases, all of which localize to the mitotic spindle pole bodies (SPBs). Two of the SIN kinases, Cdc7p and Sid1p, localize asymmetrically to the newly duplicated SPB in late anaphase. How this asymmetry is achieved is not understood, although it is known that their symmetric localization impairs cytokinesis. Here we characterize a new Forkhead-domain-associated protein, Csc1p, and identify SIN-inhibitory PP2A complex (SIP), which is crucial for the establishment of SIN asymmetry. Csc1p localizes to both SPBs early in mitosis, is lost from the SPB that accumulates Cdc7p, and instead accumulates at the SPB lacking Cdc7p. Csc1p is required for the dephosphorylation of the SIN scaffolding protein Cdc11p and is thereby required for the recruitment of Byr4p, a component of the GTPase-activating subunit for Spg1p, to the SPB. Because Cdc7p does not bind to GDP-Spg1p, we propose that the SIP-mediated Cdc11p dephosphorylation and the resulting recruitment of Byr4p are among the earliest steps in the establishment of SIN asymmetry. Copyright © 2011 Elsevier Ltd. All rights reserved.

  1. SIN-Inhibitory Phosphatase Complex (SIP) Promotes Cdc11p Dephosphorylation and Propagates SIN Asymmetry in Fission Yeast

    Science.gov (United States)

    Singh, N. Sadananda; Shao, Nan; McLean, Janel R.; Sevugan, Mayalagu; Ren, Liping; Chew, Ting Gang; Bimbo, Andrea; Sharma, Reetu; Tang, Xie; Gould, Kathleen L.; Balasubramanian, Mohan K.

    2014-01-01

    Background Cytokinesis in many eukaryotes involves the function of an actomyosin-based contractile ring. In fission yeast, actomyosin ring maturation and stability require a conserved signaling pathway termed the SIN (septation initiation network). The SIN consists of a GTPase (Spg1p) and three protein kinases, all of which localize to the mitotic spindle pole bodies (SPBs). Two of the SIN kinases, Cdc7p and Sid1p, localize asymmetrically to the newly duplicated SPB in late anaphase. How this asymmetry is achieved is not understood, although it is known that their symmetric localization impairs cytokinesis. Results Here we characterize a new Forkhead-domain-associated protein, Csc1p, and identify SIN-Inhibitory PP2A-complex (SIP), which is crucial for the establishment of SIN asymmetry. Csc1p localizes to both SPBs early in mitosis, is lost from the SPB that accumulates Cdc7p, and instead accumulates at the SPB lacking Cdc7p. Csc1p is required for the dephosphorylation of the SIN scaffolding protein Cdc11p and is thereby required for the recruitment of Byr4p, a component of the GTPase-activating subunit for Spg1p, to the SPB. Conclusions Since Cdc7p does not bind to GDP-Spg1p, we propose that the SIP-mediated Cdc11p-dephosphorylation and the resulting recruitment of Byr4p are amongst the earliest steps in the establishment of SIN asymmetry. PMID:22119525

  2. Microgravity simulation activates Cdc42 via Rap1GDS1 to promote vascular branch morphogenesis during vasculogenesis

    Directory of Open Access Journals (Sweden)

    Shouli Wang

    2017-12-01

    Full Text Available Gravity plays an important role in normal tissue maintenance. The ability of stem cells to repair tissue loss in space through regeneration and differentiation remains largely unknown. To investigate the impact of microgravity on blood vessel formation from pluripotent stem cells, we employed the embryoid body (EB model for vasculogenesis and simulated microgravity by clinorotation. We first differentiated mouse embryonic stem cells into cystic EBs containing two germ layers and then analyzed vessel formation under clinorotation. We observed that endothelial cell differentiation was slightly reduced under clinorotation, whereas vascular branch morphogenesis was markedly enhanced. EB-derived endothelial cells migrated faster, displayed multiple cellular processes, and had higher Cdc42 and Rac1 activity when subjected to clinorotation. Genetic analysis and rescue experiments demonstrated that Cdc42 but not Rac1 is required for microgravity-induced vascular branch morphogenesis. Furthermore, affinity pull-down assay and mass spectrometry identified Rap1GDS1 to be a Cdc42 guanine nucleotide exchange factor, which was upregulated by clinorotation. shRNA-mediated knockdown of Rap1GDS1 selectively suppressed Cdc42 activation and inhibited both baseline and microgravity-induced vasculogenesis. This was rescued by ectopic expression of constitutively active Cdc42. Taken together, these results support the notion that simulated microgravity activates Cdc42 via Rap1GDS1 to promote vascular branch morphogenesis.

  3. The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing

    DEFF Research Database (Denmark)

    Lischetti, Tiziana; Zhang, Gang; Sedgwick, Garry G

    2014-01-01

    Improperly attached kinetochores activate the spindle assembly checkpoint (SAC) and by an unknown mechanism catalyse the binding of two checkpoint proteins, Mad2 and BubR1, to Cdc20 forming the mitotic checkpoint complex (MCC). Here, to address the functional role of Cdc20 kinetochore localization...... in the SAC, we delineate the molecular details of its interaction with kinetochores. We find that BubR1 recruits the bulk of Cdc20 to kinetochores through its internal Cdc20 binding domain (IC20BD). We show that preventing Cdc20 kinetochore localization by removal of the IC20BD has a limited effect...... on the SAC because the IC20BD is also required for efficient SAC silencing. Indeed, the IC20BD can disrupt the MCC providing a mechanism for its role in SAC silencing. We thus uncover an unexpected dual function of the second Cdc20 binding site in BubR1 in promoting both efficient SAC signalling and SAC...

  4. A New Genetically Encoded Single-Chain Biosensor for Cdc42 Based on FRET, Useful for Live-Cell Imaging

    Science.gov (United States)

    Cox, Dianne; Hodgson, Louis

    2014-01-01

    Cdc42 is critical in a myriad of cellular morphogenic processes, requiring precisely regulated activation dynamics to affect specific cellular events. To facilitate direct observations of Cdc42 activation in live cells, we developed and validated a new biosensor of Cdc42 activation. The biosensor is genetically encoded, of single-chain design and capable of correctly localizing to membrane compartments as well as interacting with its upstream regulators including the guanine nucleotide dissociation inhibitor. We characterized this new biosensor in motile mouse embryonic fibroblasts and observed robust activation dynamics at leading edge protrusions, similar to those previously observed for endogenous Cdc42 using the organic dye-based biosensor system. We then extended our validations and observations of Cdc42 activity to macrophages, and show that this new biosensor is able to detect differential activation patterns during phagocytosis and cytokine stimulation. Furthermore, we observe for the first time, a highly transient and localized activation of Cdc42 during podosome formation in macrophages, which was previously hypothesized but never directly visualized. PMID:24798463

  5. A CDC45 Homolog in Arabidopsis Is Essential for Meiosis, as Shown by RNA Interference–Induced Gene Silencing

    Science.gov (United States)

    Stevens, Rebecca; Grelon, Mathilde; Vezon, Daniel; Oh, Jaesung; Meyer, Peter; Perennes, Claudette; Domenichini, Severine; Bergounioux, Catherine

    2004-01-01

    CDC45 is required for the initiation of DNA replication in yeast and cell proliferation in mammals and functions as a DNA polymerase α loading factor in Xenopus. We have cloned a CDC45 homolog from Arabidopsis whose expression is upregulated at the G1/S transition and in young meiotic flower buds. One-third of Arabidopsis 35S:CDC45 T1 RNA interference lines are partially to completely sterile, and the proportion of sterile plants is increased by using a dmc1 promoter. T1 plants have decreased levels of the CDC45 transcript and contain 21- to 23-bp RNA fragments specific to the CDC45 gene. T2 transgenic lines, in which small RNA fragments are still present, were used to analyze S-phase entry by 5-bromodeoxyuridine incorporation, which was not altered compared with that in the wild type. However, microarray data show that other cell cycle genes are upregulated or downregulated. T2 plants also have highly reduced fertility. The severity of the phenotype is correlated with the levels of the CDC45 transcript and small RNA fragments. Severe chromosome fragmentation arising during meiosis, which is not the result of a defect in the repair of SPO11-induced double strand breaks, leads to abnormal chromosome segregation and defective pollen and ovule development. PMID:14660803

  6. Estabilización robusta de sistemas lineales

    Directory of Open Access Journals (Sweden)

    Horacio Leyva Castellanos

    2013-12-01

    Full Text Available En este trabajo se muestra un conjunto de resultados que permiten abordar el problema de la estabilización robusta de una familia de sistemas lineales positivos. Se describen dos nuevas aplicaciones en la teoría de control positivo para sistemas compartimentales y se muestra la aplicación de un teorema de robustez para tales sistemas

  7. Sistemas territoriales de salud frente al sistema general de seguridad social en salud de Colombia

    OpenAIRE

    Jahir Alexander Gutiérrez Ossa; Rubén Darío Restrepo Avendaño

    2014-01-01

    Introducción: El artículo expone los elementos y fundamentos que podrían avalar la generación de sistemas de seguridad social en salud territorial, en complementación con el sistema general preexistente. Materiales y Métodos: La eclosión generalizada en los sistemas de salud, implica la revisión de los modelos propuestos de carácter central y territorial. El análisis económico institucional, permite analizar las condiciones que tienen los sistemas generales para impactar en los esquemas terri...

  8. La morera: una alternativa viable para los sistemas de alimentación animal en el trópico

    Directory of Open Access Journals (Sweden)

    D. García

    2006-01-01

    Full Text Available Se presenta una revisión sobre las principales características de las especies de morera y sus potencialidades como plantas forrajeras en las condiciones tropicales; se hace énfasis en los aspectos relacionados con su taxonomía, distribución geográfica, principales usos, manejo agronómico, composición química, valor nutritivo y principales metabolitos secundarios presentes en su biomasa. Las especies de morera, oriundas del continente euroasiático, pertenecen a la familia Moraceae. En ese sentido, Morus alba y Morus nigra son las mayormente estudiadas por su gran distribución mundial. Desde tiempos ancestrales la morera se ha utilizado en Asia para la alimentación del gusano que produce la seda y los estudios agronómicos realizados en la sericultura han proporcionado numerosas informaciones. Sin embargo, la influencia de los principales factores en la producción de forraje solamente ha sido estudiada en forma intensiva después de su introducción al Continente Americano a partir de 1980. La morera se caracteriza por presentar una sobresaliente producción de biomasa (2-30 tMS/ha/año, contenidos elevados de proteína cruda (>15% MS, adecuada fracción fibrosa (20-40% y digestibilidad entre 70 y 90%. La biomasa contiene agentes pronutricionales (flavonoides, cumarinas, esteroles, saponinas, lectinas y fitoestrógenos. No obstante, los contenidos de estos metabolitos no sobrepasan los niveles críticos establecidos para los rumiantes y solamente los tres últimos grupos presentan potencial antinutricional en monogástricos. En virtud de sus singulares características, las especies de morera constituyen excelentes alternativas alimentarias en los sistemas tropicales de bajos insumos.

  9. CDC25A Protein Stability Represents a Previously Unrecognized Target of HER2 Signaling in Human Breast Cancer: Implication for a Potential Clinical Relevance in Trastuzumab Treatment

    Directory of Open Access Journals (Sweden)

    Emanuela Brunetto

    2013-06-01

    Full Text Available The CDC25A-CDK2 pathway has been proposed as critical for the oncogenic action of human epidermal growth factor receptor 2 (HER2 in mammary epithelial cells. In particular, transgenic expression of CDC25A cooperates with HER2 in promoting mammary tumors, whereas CDC25A hemizygous loss attenuates the HER2-induced tumorigenesis penetrance. On the basis of this evidence of a synergism between HER2 and the cell cycle regulator CDC25A in a mouse model of mammary tumorigenesis, we investigated the role of CDC25A in human HER2-positive breast cancer and its possible implications in therapeutic response. HER2 status and CDC25A expression were assessed in 313 breast cancer patients and we found statistically significant correlation between HER2 and CDC25A (P = .007. Moreover, an HER2-positive breast cancer subgroup with high levels of CDC25A and very aggressive phenotype was identified (P = .005. Importantly, our in vitro studies on breast cancer cell lines showed that the HER2 inhibitor efficacy on cell growth and viability relied also on CDC25A expression and that such inhibition induces CDC25A down-regulation through phosphatidylinositol 3-kinase/protein kinase B pathway and DNA damage response activation. In line with this observation, we found a statistical significant association between CDC25A overexpression and trastuzumab-combined therapy response rate in two different HER2-positive cohorts of trastuzumab-treated patients in either metastatic or neoadjuvant setting (P = .018 for the metastatic cohort and P = .021 for the neoadjuvant cohort. Our findings highlight a link between HER2 and CDC25A that positively modulates HER2- targeted therapy response, suggesting that, in HER2-positive breast cancer patients, CDC25A overexpression affects trastuzumab sensitivity.

  10. Sombras no Sistema Diédrico

    OpenAIRE

    Tereno, Maria do Céu

    2011-01-01

    Complilação de material didático sobre o Estudo das sombras no sistema diédrico. Estudo dos métodos de representação. Estudo da intersecção de sólidos. Superfícies de cobertura resultantes da intersecção de sólidos.

  11. ENEA presenta sistema ADS per trattamento scorie

    CERN Multimedia

    2002-01-01

    "Arriva il sistema Ads (Accelerator driven system) per il trattamento dei rifiuti radioattivi. Lo studio di fattibilita' per l' innovativa tecnologia, realizzato da un gruppo di lavoro congiunto Enea, Cea, Cern e Ansaldo, e' stato presentato oggi nel corso di un workshop presieduto dal premio Nobel per la fisica, Carlo Rubbia" (1/2 page).

  12. Elementos del sistema electoral del Congreso Mexicano

    Directory of Open Access Journals (Sweden)

    Aldo Medina García

    2013-08-01

    Full Text Available El presente artículo es un estudio de los componentes del sistema electoral, diseñado por el legislador mexicano para integrar al parlamento federal. Estudio desarrollado a sabiendas de la importancia que reviste el sistema electoral como un medio que permite a los ciudadanos convertir su voto expresado en las urnas en escaños a favor de determinado candidato o partido. En dicho análisis tomamos en cuenta la evolución constitucional del sistema electoral mexicano, la participación de las fuerzas minoritarias en la integración del Senado y de la Cámara de Diputados, la división que se hace del territorio en circunscripciones electorales, la barrera electoral que se fija a los partidos políticos para acceder a la representación en el Parlamento, la fórmula de asignación que se aplica a la votación obtenida, para finalmente concluir que el sistema electoral mexicano es esencialmente mayoritario aun cuando se vista de proporcional.

  13. El Sistema and American Music Education

    Science.gov (United States)

    Lesniak, Melissa

    2012-01-01

    The brainchild of Jose Antonio Abreu, El Sistema, a music education program for aspiring orchestra musicians launched in Venezuela for students of limited means and now spreading to other parts of the world, has become a subject of interest to music teachers and teacher educators in North America. This article examines a bit of the program's…

  14. Los TLC de Estados Unidos con países latinoamericanos: un modelo de integración económica “superficial” para el continente americano

    Directory of Open Access Journals (Sweden)

    José Julián Tole

    2013-08-01

    Full Text Available El modelo TLC de Estados Unidos se convierte en un instrumento de integración “ideal” para mejorar sus intereses geopolíticos y geoeconómicos en el continente americano, pues facilita la construcción de peldaños o pequeños bloques que mejorarán su peso en la escena internacional y en algunos sectores del comercio internacional. En tal contexto, el objetivo de este trabajo es analizar el modelo “hecho a la medida” por Estados Unidos para regular y profundizar la liberalización comercial, sin considerar de ninguna manera un mayor grado de integración, con los países latinoamericanos.

  15. Manuel J. Peláez (dir., Diccionario de Canonistas y Eclesiasticistas Europeos y Americanos (Tomo I, AV Akademikerverlag GmbH & Co. KG, EAE, Saarbrücken, 2012, 547 págs.

    Directory of Open Access Journals (Sweden)

    Purificación Alcaide-Pulido

    2013-03-01

    Full Text Available ¿Por qué se otorga escasa atención a los canonistas y eclesiasticistas dentro del Derecho canónico y del Derecho eclesiástico? Esta es la cuestión que surge y el motivo de la aparición de la obra Diccionario de canonistas y eclesiasticistas europeos y americanos. Dicha obra aparece como una necesidad científica emanada de la escasa atención que en el ámbito citado se presta a canonistas y a eclesiasticistas, a través de una recopilación de los pensadores más influyentes en las diversas áreas que comprenden el Derecho Canónico y Eclesiástico, a lo largo del último milenio y, por ende, también de interés para iushistoricistas.

  16. Observadores Distribuidos Garantistas para Sistemas en Red

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    Ramón A. García

    2017-07-01

    Full Text Available Resumen: En este artículo se propone un observador distribuido garantista para sistemas en red, considerando de forma explícita el problema de los retardos variables en las comunicaciones. Se asume que la información intercambiada entre agentes llega siempre a su destino, si bien las comunicaciones están sujetas a retardos variables, cuyo valor máximo se supone conocido. Cada observador trabaja con información parcial, y necesita comunicarse con observadores vecinos para llevar a cabo una estimación del estado completo del sistema. Para representar a los conjuntos garantistas, cuya función es acotar en tiempo real la región en la que se encuentra el estado del sistema, se ha optado por la utilización de zonotopos. Esto permite integrar de forma sencilla la información recibida por cada agente. Finalmente se presentan resultados de simulación para validar el algoritmo propuesto. Abstract: This paper proposes a guaranteed distributed observer for networked systems, taking into account the problem of the variable delays in communications. We assume that the information exchanged among the agents always arrives to its destination, although the communication are subject to variable delays, whose maximum value is known. Each observer works with partial information, and needs to communicate with neighbouring observers to carry out an estimation of the complete state of the system. The guaranteed sets, whose function is to delimit in real time the region in which the state of the system belong to, are represented by zonotopes. This kind of sets allows a simple integration of the information received by each agent. Finally some results obtained with the proposed algorithm are shown in simulations. Palabras clave: Estimación Distribuida, Observadores de Estado, Sistemas con Retardos, Sistemas en red, Zonotopos, Keywords: Distributed Estimation, State Observers, Delays Systems, Networked systems, Zonotopes

  17. Lista actualizada y clave ilustrada para los géneros de misidáceos (Crustacea, Peracarida del Mar Intra-Americano Updated checklist and illustrated key to mysid (Crustacea, Peracarida genera from the Intra-American Sea

    Directory of Open Access Journals (Sweden)

    Manuel Ortiz

    2012-12-01

    Full Text Available Se presenta una lista actualizada y una clave ilustrada para la identificación de los 38 géneros marinos, estuarinos y estigobios de los misidáceos que habitan en el Mar Intra-Americano. Estos crustáceos incluyen 6 familias, 5 subfamilias y 5 tribus dentro de los órdenes Lophogastrida y Mysida. El primero contiene 5 géneros y el segundo 33; de éstos, 35 habitan el ambiente marino y el salobre, 2 el cárstico y 1, Antromysis, vive en cualquiera de los 3 ambientes. Los géneros Anchialina, Siriella, Mysidopsis, Mysidium y Heteromysis se distribuyen ampliamente en todo el Mar Intra-Americano. Contrariamente a Chalaraspidum, Euchaetomera, Eucopia, Gironomysis, Metamblyops, Mysidella, Palaumysis, Platymysis, Pleurerythrops, Pseudomma y Synerythrops con una distribución geográfica restringida. El Mar Caribe y el Golfo de México representan las 2 regiones biogeográficas con mayor número de géneros de misidáceos.An updated checklist and an illustrated key for the identification of the 38 genera of marine, estuarine and stygobitic opossum shrimps recorded from the Intra-American Sea, are presented. These crustaceans were included in 6 families, 5 subfamilies, and 5 tribes, into the suborders Lophogastrida and Mysida. First order grouped 5 genera and the second 33. Of these genera, 35 live in marine and estuarine environments, 2 are stygobitic and another, Antromysis, is distributed in each one. The genera Anchialina, Siriella, Mysidopsis, Mysidium and Heteromysis have a wide distribution in overall Intra-American Sea, instead of Chalaraspidum, Euchaetomera, Eucopia, Gironomysis, Metamblyops, Mysidella, Palaumysis, Platymysis, Pleurerythrops, Pseudomma and Synerythrops with a restricted geographical distribution. The Caribbean Sea and the Gulf of Mexico constitute the regions with the biggest number of mysids genera.

  18. Sistemas de Cultivo de la Papa en Antioquia

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    Arango U. Ernesto

    1948-04-01

    Full Text Available Existen tres sistemas de cultivo entre los que se encuentran el sistema de tapado, sistema de destapado y el sistema de cepillado. Es necesario aclarar que la mayor utilidad que se obtiene con el sistema de cepillado sobre el de destapado, no compensa las pérdidas del suelo, puesto que este queda inutilizado por mucho tiempo, siendo necesario abandonarlo por varios años, antes de volver a lograr una cosecha remuneradora. Además se obtienen cosechas de papa demasiado bajas lo que merma considerablemente los rendimientos de la zona.

  19. Cdc14 phosphatase directs centrosome re-duplication at the meiosis I to meiosis II transition in budding yeast.

    Science.gov (United States)

    Fox, Colette; Zou, Juan; Rappsilber, Juri; Marston, Adele L

    2017-01-05

    Background Gametes are generated through a specialized cell division called meiosis, in which ploidy is reduced by half because two consecutive rounds of chromosome segregation, meiosis I and meiosis II, occur without intervening DNA replication. This contrasts with the mitotic cell cycle where DNA replication and chromosome segregation alternate to maintain the same ploidy. At the end of mitosis, CDKs are inactivated. This low CDK state in late mitosis/G1 allows for critical preparatory events for DNA replication and centrosome/spindle pole body (SPB) duplication. However, their execution is inhibited until S phase, where further preparatory events are also prevented. This "licensing" ensures that both the chromosomes and the centrosomes/SPBs replicate exactly once per cell cycle, thereby maintaining constant ploidy. Crucially, between meiosis I and meiosis II, centrosomes/SPBs must be re-licensed, but DNA re-replication must be avoided. In budding yeast, the Cdc14 protein phosphatase triggers CDK down regulation to promote exit from mitosis. Cdc14 also regulates the meiosis I to meiosis II transition, though its mode of action has remained unclear. Methods Fluorescence and electron microscopy was combined with proteomics to probe SPB duplication in cells with inactive or hyperactive Cdc14. Results We demonstrate that Cdc14 ensures two successive nuclear divisions by re-licensing SPBs at the meiosis I to meiosis II transition. We show that Cdc14 is asymmetrically enriched on a single SPB during anaphase I and provide evidence that this enrichment promotes SPB re-duplication. Cells with impaired Cdc14 activity fail to promote extension of the SPB half-bridge, the initial step in morphogenesis of a new SPB. Conversely, cells with hyper-active Cdc14 duplicate SPBs, but fail to induce their separation. Conclusion Our findings implicate reversal of key CDK-dependent phosphorylations in the differential licensing of cyclical events at the meiosis I to meiosis I

  20. Investigation of Colorless Distributed Combustion (CDC) with Swirl for Gas Turbine Application

    Science.gov (United States)

    Khalil Hasan, Ahmed Essam ElDin

    Colorless Distributed Combustion (CDC) with swirl is investigated for gas turbine engine applications due to its benefits for ultra-low pollutants emission, improved pattern factor and thermal field uniformity, low noise emission, and stable combustion with the alleviation of combustion instabilities. Adequate and fast mixing between the injected air and internally recirculated hot reactive gases to form hot and diluted oxidant is critical for CDC, followed by rapid mixing with the fuel. This results in distributed reaction zone instead of a concentrated thin flame front as observed in conventional diffusion flames, leading to avoidance of hot spot regions and providing reduced NOx and CO emissions. The focus of this dissertation is to develop and demonstrate CDC in a cylindrical combustor for application to stationary gas turbine combustors. The dissertation examines the sequential development of ultra-low emission colorless distributed combustor operating at a nominal thermal intensity of 36MW/m3-atm. Initially, the role of swirl is evaluated through comparing the performance of swirling and non-swirling configurations with focus on pollutants emission, stability, and isothermal flowfield through particle image velocimetry. Different fuel injection locations have also been examined, and based on performance a swirling configuration have been down selected for further investigations demonstrating emissions as low as 1 PPM of NO with a 40% reduction compared to non-swirling configuration. Further investigations were performed to outline the impact of inlet air temperature and combustor pressure on reaction distribution and combustor performance. Next, Fuel flexibility has been examined with view to develop CDC combustors that can handle different gaseous and liquid fuels, both traditional and renewable. These fuels included diluted methane, hydrogen enriched methane, propane, ethanol, kerosene, JP-8, Hydrogenated Renewable Jet fuel, and novel biofuel. Swirling CDC

  1. Sequencing Analysis of Mutant Allele $cdc$28-$srm$ of Protein Kinase CDC28 and Molecular Dynamics Study of Glycine-Rich Loop in Wild-Type and Mutant Allele G16S of CDK2 as Model

    CERN Document Server

    Koltovaya, N A; Kholmurodov, Kh T; Kretov, D A

    2005-01-01

    The central role that cyclin-dependent kinases play in the timing of cell division and the high incidence of genetic alteration of CDKs or deregulation of CDK inhibitors in a number of cancers make CDC28 of the yeast \\textit{Saccharomyces cerevisiae }very attractive model for studies of mechanisms of CDK regulation. Earlier it was found that certain gene mutations including \\textit{cdc28-srm} affect cell cycle progression, maintenance of different genetic structures and increase cell sensitivity to ionizing radiation. A~\\textit{cdc28-srm} mutation is not temperature-sensitive mutation and differs from the known \\textit{cdc28-ts }mutations because it has the evident phenotypic manifestations at 30 $^{\\circ}$C. Sequencing analysis of \\textit{cdc28-srm} revealed a single nucleotide substitution G20S. This is a third glycine in a conserved sequence GxGxxG in the G-rich loop positioned opposite the activation T-loop. Despite its demonstrated importance, the role of the G-loop has remained unclear. The crystal stru...

  2. Winter hibernation and UCHL1-p34cdc2 association in toad oocyte maturation competence.

    Directory of Open Access Journals (Sweden)

    Zhichao Kuang

    Full Text Available Currently, it is believed that toad oocyte maturation is dependent on the physiological conditions of winter hibernation. Previous antibody-blocking experiments have demonstrated that toad ubiquitin carboxyl-terminal hydrolase L1 (tUCHL1 is necessary for germinal vesicle breakdown during toad oocyte maturation. In this paper, we first supply evidence that tUCHL1 is highly evolutionarily conserved. Then, we exclude protein availability and ubiquitin carboxyl-terminal hydrolase enzyme activity as factors in the response of oocytes to winter hibernation. In the context of MPF (maturation promoting factor controlling oocyte maturation and to further understand the role of UCHL1 in oocyte maturation, we performed adsorption and co-immunoprecipitation experiments using toad oocyte protein extracts and determined that tUCHL1 is associated with MPF in toad oocytes. Recombinant tUCHL1 absorbed p34(cdc2, a component of MPF, in obviously larger quantities from mature oocytes than from immature oocytes, and p13(suc1 was isolated from tUCHL1 with a dependence on the ATP regeneration system, suggesting that still other functions may be involved in their association that require phosphorylation. In oocytes from hibernation-interrupted toads, the p34(cdc2 protein level was significantly lower than in oocytes from toads in artificial hibernation, providing an explanation for the different quantities isolated by recombinant tUCHL1 pull-down and, more importantly, identifying a mechanism involved in the toad oocyte's dependence on a low environmental temperature during winter hibernation. Therefore, in toads, tUCHL1 binds p34(cdc2 and plays a role in oocyte maturation. However, neither tUCHL1 nor cyclin B1 respond to low temperatures to facilitate oocyte maturation competence during winter hibernation.

  3. Winter hibernation and UCHL1-p34cdc2 association in toad oocyte maturation competence.

    Science.gov (United States)

    Kuang, Zhichao; Yao, Yuwei; Shi, Yan; Gu, Zheng; Sun, Zhaogui; Tso, Jiake

    2013-01-01

    Currently, it is believed that toad oocyte maturation is dependent on the physiological conditions of winter hibernation. Previous antibody-blocking experiments have demonstrated that toad ubiquitin carboxyl-terminal hydrolase L1 (tUCHL1) is necessary for germinal vesicle breakdown during toad oocyte maturation. In this paper, we first supply evidence that tUCHL1 is highly evolutionarily conserved. Then, we exclude protein availability and ubiquitin carboxyl-terminal hydrolase enzyme activity as factors in the response of oocytes to winter hibernation. In the context of MPF (maturation promoting factor) controlling oocyte maturation and to further understand the role of UCHL1 in oocyte maturation, we performed adsorption and co-immunoprecipitation experiments using toad oocyte protein extracts and determined that tUCHL1 is associated with MPF in toad oocytes. Recombinant tUCHL1 absorbed p34(cdc2), a component of MPF, in obviously larger quantities from mature oocytes than from immature oocytes, and p13(suc1) was isolated from tUCHL1 with a dependence on the ATP regeneration system, suggesting that still other functions may be involved in their association that require phosphorylation. In oocytes from hibernation-interrupted toads, the p34(cdc2) protein level was significantly lower than in oocytes from toads in artificial hibernation, providing an explanation for the different quantities isolated by recombinant tUCHL1 pull-down and, more importantly, identifying a mechanism involved in the toad oocyte's dependence on a low environmental temperature during winter hibernation. Therefore, in toads, tUCHL1 binds p34(cdc2) and plays a role in oocyte maturation. However, neither tUCHL1 nor cyclin B1 respond to low temperatures to facilitate oocyte maturation competence during winter hibernation.

  4. HRPT2- (CDC73) RELATED HEREDITARY HYPERPARATHYROIDISM: A CASE SERIES FROM WESTERN INDIA.

    Science.gov (United States)

    Khadilkar, Kranti S; Budyal, Sweta R; Kasliwal, Rajiv; Lila, Anurag R; Bandgar, Tushar; Shah, Nalini S

    2015-09-01

    To describe a case series of HRPT2- (CDC73) related hereditary primary hyperparathyroidism (PHPT) from western India. We present a case series of 4 families (7 patients) with PHPT caused by CDC73 gene mutations. The mean age of presentation of the 4 index cases was 27.25 ± 9.8 years. Two family members were identified through biochemical screening (Cases 1b and 2b), while 1 mutation-positive family member did not manifest any features of PHPT or hyperparathyroidism jaw tumor syndrome (HPT-JT) syndrome (Case 2c). Biochemistry showed increased serum calcium (mean: 13.21 ± 1.24 mg/dL), low serum phosphorus (mean: 1.78 ± 0.44 mg/dL), and high parathyroid hormone (PTH, mean: 936 ± 586.9 pg/mL). All patients had a uniglandular presentation and underwent single adenoma excision initially except Cases 2a and 2b, who underwent subtotal parathyroidectomy at baseline. Two cases experienced PHPT recurrence (Cases 3 and 4), while 1 remained uncured due to parathyroid carcinoma (Case 1a). Other associated syndromic features like ossifying jaw fibromas were present in 2 patients, renal cysts in 3 patients, and uterine involvement in 2 patients. Two families had novel germline CDC73 mutations (Families 1 and 3), while the other 2 had reported mutations. Family 2 had familial isolated PHPT without any other features of HPT-JT syndrome. Our findings reaffirm the need for genetic analysis of patients with PHPT, especially those with younger age of disease onset; recurrent disease; and associated features like polycystic kidneys, endometrial involvement, ossifying jaw tumors, or parathyroid carcinoma.

  5. CDC Signos Vitales-Las medidas hospitalarias afectan la lactancia materna (Hospital Actions Affect Breastfeeding)

    Centers for Disease Control (CDC) Podcasts

    2015-10-06

    Este podcast se basa en la edición de octubre del 2015 del informe Signos Vitales de los CDC. Los hospitales pueden implementar los "Diez Pasos hacia una Feliz Lactancia Natural" para obtener la designación de "Amigo del Niño" y así apoyar a más mamás en su decisión de amamantar.  Created: 10/6/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/6/2015.

  6. CDC Signos Vitales-Seguridad de los camioneros (Vital Signs-Trucker Safety)

    Centers for Disease Control (CDC) Podcasts

    2015-03-03

    Este podcast se basa en la edición de marzo del 2015 del informe Signos Vitales de los CDC. En el 2012 en los Estados Unidos, ocurrieron cerca de 317 000 choques asociados a camiones pesados. Veintiséis mil camioneros y sus pasajeros sufrieron lesiones en esos choques, y cerca de 700 murieron. Infórmese sobre lo que se puede hacer para que los camioneros estén seguros.  Created: 3/3/2015 by National Institute for Occupational Safety and Health (NIOSH).   Date Released: 3/3/2015.

  7. CDC Signos Vitales: Mortalidad por choques automovilísticos (Motor Vehicle Crash Deaths)

    Centers for Disease Control (CDC) Podcasts

    2016-07-06

    Este podcast se basa en la edición de julio de 2016 del informe Signos Vitales de los CDC. En los Estados Unidos, aproximadamente 90 personas mueren en choques automovilísticos cada día y miles sufren lesiones, lo cual resulta en cientos de millones de dólares en costos médicos directos cada año. Sepa qué puede hacer para mantenerse seguro.  Created: 7/6/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 7/6/2016.

  8. CDC Vital Signs-Alcohol and Pregnancy: Why Take the Risk?

    Centers for Disease Control (CDC) Podcasts

    2016-02-02

    This podcast is based on the February 2016 CDC Vital Signs report. More than three million women in the U.S. are at risk for exposing their developing baby to alcohol. Drinking alcohol during pregnancy can cause physical, behavioral, and intellectual disabilities that can affect a child’s whole life. Learn what can be done to keep developing babies healthy.  Created: 2/2/2016 by National Center on Birth Defects and Developmental Disabilities (NCBDDD).   Date Released: 2/2/2016.

  9. CDC Vital Signs–Legionnaires’ Disease

    Centers for Disease Control (CDC) Podcasts

    2016-06-07

    This podcast is based on the June 2016 CDC Vital Signs report. People can get Legionnaires’ disease, a serious type of lung infection, from breathing in small water droplets of water contaminated with Legionella germs. Learn what can be done to help prevent Legionnaires’ disease outbreaks and keep people safe.  Created: 6/7/2016 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 6/7/2016.

  10. CDC: Tips from Former Smokers – Tiffany PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    2013-03-28

    When Tiffany was 16, her mother—a cigarette smoker—died of lung cancer. Tiffany quit smoking at 34 because she wanted to be around for her own daughter, who had just turned 16. In this 60 second PSA from CDC's Tips From Former Smokers campaign, Tiffany offers tips on how to quit.  Created: 3/28/2013 by Office on Smoking and Health, National Center for Chronic Disease Prevention and Health Promotion.   Date Released: 8/8/2013.

  11. Signos Vitales de los CDC-El humo de segunda mano (Secondhand Smoke)

    Centers for Disease Control (CDC) Podcasts

    2015-02-03

    Este podcast se basa en la edición de febrero del 2015 del informe de Signos Vitales de los CDC. El humo de segunda mano mata a más de 400 bebés y 41 000 adultos no fumadores al año. Sepa qué se puede hacer para prevenir la exposición al humo de segunda mano.  Created: 2/3/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 2/3/2015.

  12. Unraveling the molecular mechanism of interactions of the Rho GTPases Cdc42 and Rac1 with the scaffolding protein IQGAP2.

    Science.gov (United States)

    Ozdemir, E Sila; Jang, Hyunbum; Gursoy, Attila; Keskin, Ozlem; Li, Zhigang; Sacks, David B; Nussinov, Ruth

    2018-03-09

    IQ motif-containing GTPase-activating proteins (IQGAPs) are scaffolding proteins playing central roles in cell-cell adhesion, polarity, and motility. The Rho GTPases Cdc42 and Rac1, in their GTP-bound active forms, interact with all three human IQGAPs. The IQGAP-Cdc42 interaction promotes metastasis by enhancing actin polymerization. However, despite their high sequence identity, Cdc42 and Rac1 differ in their interactions with IQGAP. Two Cdc42 molecules can bind to the Ex-domain and the RasGAP site of the GTPase-activating protein (GAP)-related domain (GRD) of IQGAP and promote IQGAP dimerization. Only one Rac1 molecule might bind to the RasGAP site of GRD and may not facilitate the dimerization, and the exact mechanism of Cdc42 and Rac1 binding to IQGAP is unclear. Using all-atom molecular dynamics simulations, site-directed mutagenesis, and Western blotting, we unraveled the detailed mechanisms of Cdc42 and Rac1 interactions with IQGAP2. We observed that Cdc42 binding to the Ex-domain of GRD of IQGAP2 (GRD2) releases the Ex-domain at the C-terminal region of GRD2, facilitating IQGAP2 dimerization. Cdc42 binding to the Ex-domain promoted allosteric changes in the RasGAP site, providing a binding site for the second Cdc42 in the RasGAP site. Of note, the Cdc42 "insert loop" was important for the interaction of the first Cdc42 with the Ex-domain. By contrast, differences in Rac1 insert-loop sequence and structure precluded its interaction with the Ex-domain. Rac1 could bind only to the RasGAP site of apo-GRD2 and could not facilitate IQGAP2 dimerization. Our detailed mechanistic insights help decipher how Cdc42 can stimulate actin polymerization in metastasis.

  13. Intrinsic GTP hydrolysis is observed for a switch 1 variant of Cdc42 in the presence of a specific GTPase inhibitor

    Science.gov (United States)

    Morris, Kyla M.; Henderson, Rory; Suresh Kumar, Thallapuranam Krishnaswamy; Heyes, Colin D.; Adams, Paul D.

    2016-01-01

    ABSTRACT The Ras-related protein Cell division cycle 42 (Cdc42) is important in cell-signaling processes. Protein interactions involving Cdc42 occur primarily in flexible “Switch” regions that help regulate effector binding. We studied the kinetics of intrinsic GTP hydrolysis reaction in the absence and presence of a biologically active peptide derivative of a p21-activated kinase effector (PBD46) for wt Cdc42 and compared it to the Switch 1 variant Cdc42(T35A). While the binding of PBD46 to wt Cdc42 results in complete inhibition of GTP hydrolysis, this interaction in Cdc42(T35A) does not. Comparison of the crystal structure of wt Cdc42 in the absence of effector (1AN0.pdb), as well as the NMR structure of wt Cdc42 bound to an effector in the Switch 1 region (1CF4.pdb) (www.rcsb.org) suggests that the orientation of T35 with bound Mg2+ changes in the presence of effector, resulting in movement of GTP away from the catalytic box leading to the inhibition of GTP hydrolysis. For Cdc42(T35A), molecular dynamics simulations and structural analyses suggest that the nucleotide does not undergo the conformational shift observed for the wt Cdc42-effector interaction. Our data suggest that change in dynamics in the Switch 1 region of Cdc42 caused by the T35A mutation (Chandrashekar, et al. 2011, Biochemistry, 50, p. 6196) fosters a conformation for this Cdc42 variant that allows hydrolysis of GTP in the presence of PBD46, and that alteration of the conformational dynamics could potentially modulate Ras-related over-activity. PMID:26828437

  14. Improving integration and coordination of funding, technical assistance, and reporting/data collection: recommendations from CDC and USAPI stakeholders.

    Science.gov (United States)

    Ka'opua, Lana Sue I; White, Susan F; Rochester, Phyllis F; Holden, Debra J

    2011-03-01

    Current US Federal funding mechanisms may foster program silos that disable sharing of resources and information across programs within a larger system of public health services. Such silos present challenges to USAPI communities where human resources, health infrastructure, and health financing are limited. Integrative and coordinated approaches have been recommended. The CDC Pacific Islands Integration and Coordination project was initiated by the CDC Division of Cancer Prevention and Control (DCPC). The project aim was to identify ways for the CDC to collaborate with the USAPI in improving CDC activities and processes related to chronic disease. This article focuses on recommendations for improving coordination and integration in three core areas of health services programming: funding, program reporting/data collection and analysis, and technical assistance. Preliminary information on challenges and issues relevant to the core areas was gathered through site visits, focus groups, key informant interviews, and other sources. This information was used by stakeholder groups from the CDC and the USAPI to develop recommendations in the core programming areas. Recommendations generated at the CDC and USAPI stakeholder meetings were prepared into a single set of recommendations and stakeholders reviewed the document for accuracy prior to its dissemination to CDC's National Center for Chronic Disease Prevention and Health Promotion programs management and staff. Key recommendations, include: (1) consideration of resources and other challenges unique to the USAPI when reviewing funding applications, (2) consideration of ways to increase flexibility in USAPI use of program funds, (3) dedication of funding and human resources for technical assistance, (4) provision of opportunities for capacity-building across programs and jurisdictions, (5) consideration of ways to more directly link program reporting with technical assistance. This project provided a unique opportunity

  15. MiR-27a Promotes Hemin-Induced Erythroid Differentiation of K562 Cells by Targeting CDC25B

    Directory of Open Access Journals (Sweden)

    Dongsheng Wang

    2018-03-01

    Full Text Available Background/Aims: MicroRNAs (miRNAs play a crucial role in erythropoiesis. MiR-23a∼27a∼24-2 clusters have been proven to take part in erythropoiesis via some proteins. CDC25B (cell division control Cdc2 phosphostase B is also the target of mir-27a; whether it regulates erythropoiesis and its mechanism are unknown. Methods: To evaluate the potential role of miR-27a during erythroid differentiation, we performed miR-27a gain- and loss-of-function experiments on hemin-induced K562 cells. We detected miR-27a expression after hemin stimulation at different time points. At the same time, the γ-globin gene also was measured via real-time PCR. According to the results of the chips, we screened the target protein of miR-27a through a dual-luciferase reporter assay and identified it via Western blot analyses. To evaluate the function of CDC25B, benzidine staining and flow cytometry were employed to detect the cell differentiation and cell cycle. Results: We found that miR-27a promotes hemin-induced erythroid differentiation of human K562 cells by targeting cell division cycle 25 B (CDC25B. Overexpression of miR-27a promotes the differentiation of hemin-induced K562 cells, as demonstrated by γ-globin overexpression. The inhibition of miR-27a expression suppresses erythroid differentiation, thus leading to a reduction in the γ-globin gene. CDC25B was identified as a new target of miR-27a during erythroid differentiation. Overexpression of miR-27a led to decreased CDC25B expression after hemin treatment, and CDC25B was up-regulated when miR-27a expression was inhibited. Moreover, the inhibition of CDC25B affected erythroid differentiation, as assessed by γ-globin expression. Conclusion: This study is the first report of the interaction between miR-27a and CDC25B, and it improves the understanding of miRNA functions during erythroid differentiation.

  16. Cdc14 Early Anaphase Release, FEAR, Is Limited to the Nucleus and Dispensable for Efficient Mitotic Exit.

    Directory of Open Access Journals (Sweden)

    Christopher M Yellman

    Full Text Available Cdc14 phosphatase is a key regulator of exit from mitosis, acting primarily through antagonism of cyclin-dependent kinase, and is also thought to be important for meiosis. Cdc14 is released from its sequestration site in the nucleolus in two stages, first by the non-essential Cdc Fourteen Early Anaphase Release (FEAR pathway and later by the essential Mitotic Exit Network (MEN, which drives efficient export of Cdc14 to the cytoplasm. We find that Cdc14 is confined to the nucleus during early mitotic anaphase release, and during its meiosis I release. Proteins whose degradation is directed by Cdc14 as a requirement for mitotic exit (e.g. the B-type cyclin, Clb2, remain stable during mitotic FEAR, a result consistent with Cdc14 being restricted to the nucleus and not participating directly in mitotic exit. Cdc14 released by the FEAR pathway has been proposed to have a wide variety of activities, all of which are thought to promote passage through anaphase. Proposed functions of FEAR include stabilization of anaphase spindles, resolution of the rDNA to allow its segregation, and priming of the MEN so that mitotic exit can occur promptly and efficiently. We tested the model for FEAR functions using the FEAR-deficient mutation net1-6cdk. Our cytological observations indicate that, contrary to the current model, FEAR is fully dispensable for timely progression through a series of anaphase landmarks and mitotic exit, although it is required for timely rDNA segregation. The net1-6cdk mutation suppresses temperature-sensitive mutations in MEN genes, suggesting that rather than activating mitotic exit, FEAR either inhibits the MEN or has no direct effect upon it. One interpretation of this result is that FEAR delays MEN activation to ensure that rDNA segregation occurs before mitotic exit. Our findings clarify the distinction between FEAR and MEN-dependent Cdc14 activities and will help guide emerging quantitative models of this cell cycle transition.

  17. Identification of the quinolinedione inhibitor binding site in Cdc25 phosphatase B through docking and molecular dynamics simulations.

    Science.gov (United States)

    Ge, Yushu; van der Kamp, Marc; Malaisree, Maturos; Liu, Dan; Liu, Yi; Mulholland, Adrian J

    2017-11-01

    Cdc25 phosphatase B, a potential target for cancer therapy, is inhibited by a series of quinones. The binding site and mode of quinone inhibitors to Cdc25B remains unclear, whereas this information is important for structure-based drug design. We investigated the potential binding site of NSC663284 [DA3003-1 or 6-chloro-7-(2-morpholin-4-yl-ethylamino)-quinoline-5, 8-dione] through docking and molecular dynamics simulations. Of the two main binding sites suggested by docking, the molecular dynamics simulations only support one site for stable binding of the inhibitor. Binding sites in and near the Cdc25B catalytic site that have been suggested previously do not lead to stable binding in 50 ns molecular dynamics (MD) simulations. In contrast, a shallow pocket between the C-terminal helix and the catalytic site provides a favourable binding site that shows high stability. Two similar binding modes featuring protein-inhibitor interactions involving Tyr428, Arg482, Thr547 and Ser549 are identified by clustering analysis of all stable MD trajectories. The relatively flexible C-terminal region of Cdc25B contributes to inhibitor binding. The binding mode of NSC663284, identified through MD simulation, likely prevents the binding of protein substrates to Cdc25B. The present results provide useful information for the design of quinone inhibitors and their mechanism of inhibition.

  18. Field evaluation of CDC and Mosquito Magnet X traps baited with dry ice, CO2 sachet, and octenol against mosquitoes.

    Science.gov (United States)

    Xue, Rui-De; Doyle, Melissa A; Kline, Daniel L

    2008-06-01

    The Centers for Disease Control and Prevention (CDC) light traps and Mosquito Magnet X (MMX) traps baited with dry ice, octenol, and a new formulation (granular) of carbon dioxide (CO2) were evaluated against adult mosquitoes in the field. The results showed that the MMX traps (68.6%) baited with dry ice collected more mosquitoes compared to the CDC light traps (32.4%) only. The CDC traps baited with dry ice (64%) collected significantly more mosquitoes than traps baited with CO2 sachets (11%) or octenol (23%). The MMX traps baited with dry ice (85.5%) collected significantly more mosquitoes than traps baited with CO2 sachets (6.5%) or octenol (9%). The CDC traps baited with the formulations of normal and slow release CO2 sachets collected more mosquitoes than the formulation of fast release sachets. The CDC traps baited with fresh sachets and 24-h-exposed sachets collected significantly more mosquitoes than the traps baited with 48-h- and 72-h-exposed sachets.

  19. Sistemas electorales y justicia electoral a debate

    Directory of Open Access Journals (Sweden)

    Dieter Nohlen

    2015-07-01

    Full Text Available Despliega conceptos y planteamientos en torno a los sistemas electorales y la justicia electoral. El autor inicia con una descripción estricta de los sistemas electorales para orientar, en una segunda parte, un debate sobre la justicia electoral, que marca dos tendencias: la judicialización de la política y la politización de la administración y la jurisdicción electoral. Concluye que el desarrollo de la justicia electoral en América Latina, en sus múltiples dimensiones, es comprensible sólo con referencia al contexto sociocultural y político de la región

  20. Jaridonin-induced G2/M phase arrest in human esophageal cancer cells is caused by reactive oxygen species-dependent Cdc2-tyr15 phosphorylation via ATM–Chk1/2–Cdc25C pathway

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Yong-Cheng [Clinical Pharmacology Laboratory, Henan Province People' s Hospital, No. 7, Wei Wu Road, Zhengzhou, Henan (China); Su, Nan [Department of Quality Detection and Management, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan (China); Shi, Xiao-Jing; Zhao, Wen; Ke, Yu [School of Pharmaceutical Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, Henan (China); Zi, Xiaolin [Department of Urology, University of California, Irvine, Orange, CA (United States); Department of Pharmacology, University of California, Irvine, Orange, CA (United States); Department of Pharmaceutical Sciences, University of California, Irvine, Orange, CA (United States); Zhao, Ning-Min; Qin, Yu-Hua; Zhao, Hong-Wei [Clinical Pharmacology Laboratory, Henan Province People' s Hospital, No. 7, Wei Wu Road, Zhengzhou, Henan (China); Liu, Hong-Min, E-mail: liuhm@zzu.edu.cn [School of Pharmaceutical Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, Henan (China)

    2015-01-15

    Jaridonin, a novel diterpenoid from Isodon rubescens, has been shown previously to inhibit proliferation of esophageal squamous cancer cells (ESCC) through G2/M phase cell cycle arrest. However, the involved mechanism is not fully understood. In this study, we found that the cell cycle arrest by Jaridonin was associated with the increased expression of phosphorylation of ATM at Ser1981 and Cdc2 at Tyr15. Jaridonin also resulted in enhanced phosphorylation of Cdc25C via the activation of checkpoint kinases Chk1 and Chk2, as well as in increased phospho-H2A.X (Ser139), which is known to be phosphorylated by ATM in response to DNA damage. Furthermore, Jaridonin-mediated alterations in cell cycle arrest were significantly attenuated in the presence of NAC, implicating the involvement of ROS in Jaridonin's effects. On the other hand, addition of ATM inhibitors reversed Jaridonin-related activation of ATM and Chk1/2 as well as phosphorylation of Cdc25C, Cdc2 and H2A.X and G2/M phase arrest. In conclusion, these findings identified that Jaridonin-induced cell cycle arrest in human esophageal cancer cells is associated with ROS-mediated activation of ATM–Chk1/2–Cdc25C pathway. - Highlights: • Jaridonin induced G2/M phase arrest through induction of redox imbalance. • Jaridonin increased the level of ROS through depleting glutathione in cell. • ATM–Chk1/2–Cdc25C were involved in Jaridonin-induced cell cycle arrest. • Jaridonin selectively inhibited cancer cell viability and cell cycle progression.