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Sample records for cdc americano sistema

  1. Sistema privado de seguro-saúde: lições do caso americano

    Directory of Open Access Journals (Sweden)

    Mônica Viegas Andrade

    2000-01-01

    Full Text Available O mercado de serviços de atenção à saúde apresenta particularidades não observadas em outros mercados de bens, traduzidas nas relações contratuais estabelecidas entre os agentes deste mercado: relação paciente-provedor e relação seguradora-provedor. Na relação seguradora-provedor existe um problema de risco moral que cria incentivos à sobreutilização de serviços médicos. O objetivo deste trabalho é sistematizar as principais características institucionais do sistema de saúde americano, enfatizando as reformas ocorridas na década de 1970 e os problemas identificados em regulamentar o setor privado de serviços médicos e hospitalares. Estes problemas estão relacionados, em sua grande maioria, à regulamentação dos contratos permitidos entre as seguradoras, os provedores de serviços médicos e hospitalares e os segurados. A principal conclusão deste trabalho é mostrar que a flexibilização dos contratos entre provedores e seguradora tem efeito significativo na redução dos custos do seguro-saúde, resultando sobretudo em maior eficiência dos serviços ofertados. Os novos desenhos de contratos estabelecidos permitem a divisão do risco entre os provedores e seguradora, além de centralizar a decisão de encaminhamentos médicos a serem realizados configurando utilização mais racional destes serviços. A flexibilização dos contratos não significou, no entanto, eliminação da regulação por parte do Estado do setor de seguro-saúde privado, sendo fundamental para garantir a sustentabilidade financeira dos seguros.The aim of this paper is to organize the main institutional characteristics of the American Health System, emphasizing the reforms that occurred during the age of seventy and the difficulties to regulate the private sector of medical and hospital services. The most part of these problems are related to the contract regulation between the providers of medical and hospital service and the patients. The

  2. Potenciais benefícios do sistema de rastreabilidade animal dos EUA para o setor de carnes americano

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    Moisés de Andrade Resende Filho

    2008-12-01

    Full Text Available Este artigo investigou os potenciais ganhos do setor de carnes americano advindos da implantação do Sistema Nacional de Identificação Animal (NAIS, dos EUA. Foram analisados os potenciais efeitos do NAIS sobre a percepção de risco dos consumidores americanos em relação aos perigos decorrentes do consumo das carnes bovina, suína e de aves e seus derivados. Sistemas de equações de demanda foram estimados, incorporando-se como "proxies" da percepção de risco do consumidor, séries de índices de segurança do alimento, separadamente construídas para cada tipo de carne. Tais séries foram concebidas, somando-se o número de referências nos principais jornais americanos a problemas de segurança da carne. Foi utilizado o melhor modelo estimado, escolhido com base em uma série de testes de especificação, para se construir três cenários, simulando-se os casos em que o NAIS não está implementado; que está implementado apenas para o gado bovino; e que está implementado para suínos e bovinos. Foram utilizadas as diferenças entre as receitas estimadas para cada cenário e para cada tipo de carne, como uma medida do potencial ganho advindo da implementação do NAIS. Foi concluído que os setores da carne bovina e suína poderiam arcar com os custos do NAIS. Esse resultado, contudo, depende de o quanto desses potenciais ganhos chegarão efetivamente aos produtores agrícolas.This article investigates the potential gains to the U.S. meat sector with the implantation of the U.S. National Animal Identification System (NAIS. The focus is on the effect that the NAIS could have on consumers' risk perception about eating meat. System of demand equations are estimated using time series of food safety indexes variables used as proxies for consumers' reactions to news on meat safety issues. The series of food safety indexes are built on the basis of the number of food safety news reported in top U.S. newspapers. Using the preferred model

  3. La justicia constitucional ante el siglo XXI: la progresiva convergencia de los sistemas americano y europeo-kelseniano (extracto del libro: La Constitución Española en el contexto constitucional europeo. Editor: Francisco Fernández Segado

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    Francisco Fernández Segado

    2014-01-01

    Full Text Available 1. Reflexiones preliminares 2. La última «ratio» de la bipolaridad: la concepción del «legislador negativo» y la reafirmación kelseniana del principio de sujeción de los jueces a la ley 3. La obsolescencia de la clásica polaridad «sistema americano» versus «sistema europeo-kelseniano» 4. La centralidad del modelo americano en el proceso de convergencia de los dos tradicionales sistemas de control de constitucionalidad 5. La quiebra frontal de la concepción kelseniana del «legislador negativo» 6. La mixtura e hibridación de los actuales sistemas jurisdiccionales de control de constitucionalidad 7. A la búsqueda de una nueva tipología explicativa de las variables conformadoras del control de la constitucionalidad

  4. Investimentos estrangeiros nos sistemas financeiros latino-americanos: os casos da Argentina, do Brasil e do México Foreigner's investments in the Latin-American financial systems: the experiences of Argentina, Brazil and Mexico

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    Maria Cristina Penido de Freitas

    2008-08-01

    Full Text Available Desde o retorno dos fluxos de capitais voluntários para a América Latina nos anos 1990 ocorreu a intensificação dos investimentos estrangeiros nos mercados financeiros locais, os quais assumiram, notadamente, as formas de fluxos de portfólio e de investimento direto mediante a aquisição de participação acionária nas instituições financeiras domésticas. Argentina, Brasil e México foram os três países que mais absorveram esses fluxos destinados à região. O objetivo deste artigo é analisar os efeitos desses investimentos sobre os sistemas financeiros domésticos desses três países latino-americanos, que, longe de serem homogêneos, se diferenciaram em função do grau de abertura financeira e da gestão macroeconômica.In the 90's, the context of the renewal of capital flows to Latin America, the foreigner's investments in the local financial markets intensified not only as portfolio investments but as direct stock ownership of the financial enterprises. But, this movement is so far to be homogeneous since there are wide differences in the financial openness degrees and the macroeconomics policies across the countries. For this, we examine the effects of this process on the three Latin-American financial systems - the Brazilian, the Argentinean and Mexican ones - which have attracted the majority of the capital inflows to the region.

  5. Ukola Club. Bar americano

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    Azpiazu, J. R.

    1961-03-01

    Full Text Available En la calle de Serrano, aprovechando un semisótano dedicado a otro negocio anteriormente, se ha instalado un bar americano, de cuyo interior ofrecemos algunos pormenores. Se han cuidado, especialmente, las condiciones acústicas, resueltas por medio de un techo de escayola perforada, con vitrofib en su parte superior, y paredes de madera, que contribuyen a darle un ambiente cálido y acogedor. El soporte de hierro laminado existente en el centro del local, cuya supresión hubiera sido costosa, se ha revestido con lajas de mármol que le convierten en un elemento decorativo.

  6. Nota técnica sobre el sistema nacional de innovación de Costa Rica: Una contribución al diálogo de políticas públicas entre el Gobierno de La República de Costa Rica y el Banco Inter-Americano de Desarrollo

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    Gustavo Crespi; Rodrigo Fuentes; Roberto Alvarez; Jeffrey Orozco

    2010-01-01

    La siguiente nota técnica presenta una revisión del sistema de innovación de Costa Rica con especial énfasis en sus principales fortalezas y debilidades. El objetivo de la misma es contribuir al diálogo de políticas entre el Gobierno de Costa Rica y el Banco Inter- Americano de Desarrollo (BID) de cara al próximo período presidencial. El marco conceptual adoptado para la presente revisión esencialmente parte del concepto de innovación como la introducción de productos, procesos, servicios o m...

  7. El escritor americano

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    Cristina Iglesia

    2006-04-01

    Full Text Available Facundo se escribe para los no saben leer América. Este es uno de los puntos de partida de Sarmiento : hay una mala lectura europea de América, sobre todo de la Argentina, del Río de la Plata, de esta parte de América que es, en realidad, la única que a Sarmiento le interesa. “Sus más hábiles políticos no han alcanzado a comprender nada de lo que sus ojos han visto al echar una mirada precipitada sobre el poder americano que desafiaba a la gran nación” escribe en Viajes.Y Europa no sabe leer ...

  8. Sistema

    OpenAIRE

    Martín Serrano, Manuel

    1982-01-01

    Los términos "Modelo" y "Sistema" son dos temas escritos por Manuel Martín Serrano para la docencia universitaria. De cada uno de ellos, se ha depositado en E-Prints la versión más difundida. Proporcionan conceptos y categorías que hacen posible la aplicación de las metodologías sistémicas en la investigación de las ciencias sociales. El capítulo "Sistema" incluye los siguientes epígrafes: I. El estudio de la organización y el funcionamiento de los sistemas: 1. Concepto de sistema. 2. Análisi...

  9. sistemas

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    Diego Alejandro Muñoz Gaviria

    2006-01-01

    Full Text Available El texto presentado a continuación, plantea en términos generales las principales posturas conceptuales del sociólogo alemán Niklas Luhmann sobre las ideas de Max Weber y su teoría de la acción social, y de Talcott Parsons y sus planteamientos en torno a la teoría de los sistemas sociales. Al final del escrito se hace especial énfasis en el rol protagónico que para Luhmann tienen las ciencias sociales y humanas en la autorreferencia del sistema social.

  10. Pela democracia dos sistemas de avaliação da produção acadêmica: convergências de estudiosos latino-americanos e europeus

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    Aimée Vega Montiel

    2013-01-01

    Full Text Available El presente texto es la transcripción de una conferencia pronunciada en 2011, en el encuentro anual de la Europeean Comunication Research and Education Association (ECREA, que plantea la discusión sobre los efectos de la supremacía de los investigadores del norte sobre los investigadores del sur en el contexto de la evaluación de la calidad de la producción académica. Aunque se reconozcan las especificidades locales y regionales, así como la necesidad de adopción de diferentes metodologías e instrumentos de investigación para el estudio de problemas distintos, en contextos sociales distintos, la producción académica de los estudiosos latinoamericanos sigue siendo sometida a patrones definidos en otras latitudes del mundo. Se argumenta que la supremacía científica de los investigadores del norte se debe, fundamentalmente, a la supremacía del inglés en el sistema académico internacional, que limita las posibilidades de diseminación del conocimiento en otras lenguas.

  11. La Escuela de Estudios Hispano-Americanos

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    Bernabéu Albert, Salvador

    2010-01-01

    La Escuela de Estudios Hispano-Americanos es una institución cultural, perteneciente al Consejo Superior de Investigaciones Científicas, dedicada al estudio del Americanismo desde 1942 a la actualidad. Está encuadrada en el sector de Humanidades, una de las ocho áreas científico-técnicas en que se organiza el CSIC, el mayor organismo público de investigación en España. Paralelamente a los profundos cambios que están ocurriendo en la Universidad española con el Plan de ...

  12. CDC Disease Detective Camp

    Centers for Disease Control (CDC) Podcasts

    2010-08-02

    The CDC Disease Detective Camp gives rising high school juniors and seniors exposure to key aspects of the CDC, including basic epidemiology, infectious and chronic disease tracking, public health law, and outbreak investigations. The camp also helps students explore careers in public health.  Created: 8/2/2010 by Centers for Disease Control and Prevention (CDC).   Date Released: 8/2/2010.

  13. CDC Child Growth Charts

    Data.gov (United States)

    U.S. Department of Health & Human Services — CDC child growth charts consist of a series of percentile curves that illustrate the distribution of selected body measurements in U.S. children. Pediatric growth...

  14. CDC WONDER: Births

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Births (Natality) online databases in CDC WONDER report birth rates, fertility rates and counts of live births occurring within the United States to U.S....

  15. Recensão de "Cadernos de estudos latino-americanos nº4"

    OpenAIRE

    Cardoso, João Casqueira

    2010-01-01

    Recensão - TOSCANO, Ana María da Costa, org. - Cadernos de estudos latino-americanos : publicação do CELA, Centro de Estudos Latino-Americanos. Porto: Edições Universidade Fernando Pessoa, 2008. p. 134. ISSN 1646-5164

  16. Announcement: CDC's 70th Anniversary.

    Science.gov (United States)

    2016-01-01

    July 1, 2016, marks the 70th anniversary of the establishment of CDC. Since the agency's launch as the Communicable Disease Center on July 1, 1946, CDC's primary mission has been improving public health in the United States and around the world through prevention and preparedness. In the seven decades since CDC's founding, the agency has grown in size and mission and is recognized as the nation's premiere health promotion, disease prevention, and emergency preparedness agency, and a global leader in public health. CDC's mission has progressed beyond communicable disease control and now encompasses noninfectious diseases, injury prevention, and environmental and occupational health. PMID:27362420

  17. CDC Lab Values

    Centers for Disease Control (CDC) Podcasts

    2015-02-02

    More than fifteen hundred scientists fill the lab benches at CDC, logging more than four million hours each year. CDC’s laboratories play a critical role in the agency’s ability to find, stop, and prevent disease outbreaks. This podcast provides a brief overview of what goes on inside CDC’s labs, and why this work makes a difference in American’s health.  Created: 2/2/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 2/2/2015.

  18. Encontrando os feminismos latino-americanos e caribenhos

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    Alvarez Sonia E

    2003-01-01

    Full Text Available Este artigo examina os Encontros Latino-Americanos e do Caribe como espaços críticos transnacionais onde se re-imagina a política dos feminismos na região. Enfocando o Oitavo desses Encontros, realizado em Juan Dolio, República Dominicana, em 1999, analisamos os principais debates politicos e filosóficos que surgiram durante 20 anos de Encontros: (1 mudanças nas concepções de 'autonomia' do movimento e na relação dos feminismos com o movimento de mulheres mais amplo e com outros atores na sociedade civil e política, o Estado e instituições internacionais; (2 controvérsias geradas pelas recorrentes crises de 'inclusão' e de 'expansão' do movimento; e (3 debates centrados nas diferenças, desigualdades e desequilíbrios de poder entre mulheres em geral e entre as feministas em particular.

  19. CDC WONDER: Mortality - Infant Deaths

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Infant Deaths (from Linked Birth / Infant Death Records) online databases on CDC WONDER provide counts and rates for deaths of children under 1 year...

  20. CDC PRAMStat Data for 2000

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  1. CDC PRAMStat Data for 2011

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  2. CDC PRAMStat Data for 2001

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  3. CDC PRAMStat Data for 2010

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  4. CDC PRAMStat Data for 2003

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  5. CDC PRAMStat Data for 2005

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  6. CDC PRAMStat Data for 2008

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  7. CDC PRAMStat Data for 2002

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  8. CDC PRAMStat Data for 2004

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  9. CDC PRAMStat Data for 2007

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  10. CDC PRAMStat Data for 2009

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  11. CDC PRAMStat Data for 2006

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). PRAMS, the Pregnancy Risk Assessment Monitoring System, is a surveillance system collecting state-specific,...

  12. Stalking SARS: CDC at Work

    Centers for Disease Control (CDC) Podcasts

    2014-05-22

    In this podcast for kids, the Kidtastics talk about the SARS outbreak and how CDC worked to solve the mystery.  Created: 5/22/2014 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 5/22/2014.

  13. CDC Vital Signs: Legionnaires' Disease

    Science.gov (United States)

    ... Overview CDC investigated the first outbreak of Legionnaires’ disease, a serious lung infection (pneumonia), in 1976. An increasing number ... they develop symptoms of pneumonia. Test for Legionnaires’ disease in people with serious pneumonia, especially those requiring intensive care or who ...

  14. Phosphorylation of Cdc5 regulates its accumulation

    Directory of Open Access Journals (Sweden)

    Simpson-Lavy Kobi J

    2011-12-01

    Full Text Available Abstract Background Cdc5 (polo kinase/Plk1 is a highly conserved key regulator of the S. cerevisiae cell cycle from S-phase until cytokinesis. However, much of the regulatory mechanisms that govern Cdc5 remain to be determined. Cdc5 is phosphorylated on up to 10 sites during mitosis. In this study, we investigated the function of phosphorylation site T23, the only full consensus Cdk1 (Cdc28 phosphorylation site present. Findings Cdc5T23A introduces a degron that reduces its cellular amount to undetectable levels, which are nevertheless sufficient for normal cell proliferation. The degron acts in cis and is reversed by N-terminal GFP-tagging. Cdk1 kinase activity is required to maintain Cdc5 levels during G2. This, Cdk1 inhibited, Cdc5 degradation is APC/CCdh1 independent and requires new protein synthesis. Cdc5T23E is hyperactive, and reduces the levels of Cdc5 (in trans and drastically reduces Clb2 levels. Conclusions Phosphorylation of Cdc5 by Cdk1 is required to maintain Cdc5 levels during G2. However, phosphorylation of T23 (probably by Cdk1 caps Cdc5 and other CLB2 cluster protein accumulation, preventing potential protein toxicity, which may arise from their overexpression or from APC/CCdh1 inactivation.

  15. FUTEBOL AMERICANO NO PAÍS DO FUTEBOL: O CASO DO CUIABÁ ARSENAL

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    Francisco Xavier Freire Rodrigues

    2015-01-01

    Full Text Available O estudo aborda o processo de esportivização do futebol americano em Mato Grosso a partir da trajetória do Cuiabá Arsenal. Trata-se de uma pesquisa qualitativa, exploratória, com delineamento de estudo de caso. Dentre os resultados, verifica-se que a esportivização do Futebol Americano em Cuiabá deu-se por fases: amadorismo, transição, profissional. A equipe inspirou a criação de outras agremiações. Mesmo em época preparativa para Copa do Mundo, com intensa publicidade pelo governo do estado para divulgar o futebol “brasileiro”, o futebol “americano” é assunto da agenda esportiva midiática e cotidiana. Fato que demonstra como é possível ser esportivo.

  16. Functional characterization and cellular dynamics of the CDC-42 - RAC - CDC-24 module in Neurospora crassa.

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    Cynthia L Araujo-Palomares

    Full Text Available Rho-type GTPases are key regulators that control eukaryotic cell polarity, but their role in fungal morphogenesis is only beginning to emerge. In this study, we investigate the role of the CDC-42 - RAC - CDC-24 module in Neurospora crassa. rac and cdc-42 deletion mutants are viable, but generate highly compact colonies with severe morphological defects. Double mutants carrying conditional and loss of function alleles of rac and cdc-42 are lethal, indicating that both GTPases share at least one common essential function. The defects of the GTPase mutants are phenocopied by deletion and conditional alleles of the guanine exchange factor (GEF cdc-24, and in vitro GDP-GTP exchange assays identify CDC-24 as specific GEF for both CDC-42 and RAC. In vivo confocal microscopy shows that this module is organized as membrane-associated cap that covers the hyphal apex. However, the specific localization patterns of the three proteins are distinct, indicating different functions of RAC and CDC-42 within the hyphal tip. CDC-42 localized as confined apical membrane-associated crescent, while RAC labeled a membrane-associated ring excluding the region labeled by CDC42. The GEF CDC-24 occupied a strategic position, localizing as broad apical membrane-associated crescent and in the apical cytosol excluding the Spitzenkörper. RAC and CDC-42 also display distinct localization patterns during branch initiation and germ tube formation, with CDC-42 accumulating at the plasma membrane before RAC. Together with the distinct cellular defects of rac and cdc-42 mutants, these localizations suggest that CDC-42 is more important for polarity establishment, while the primary function of RAC may be maintaining polarity. In summary, this study identifies CDC-24 as essential regulator for RAC and CDC-42 that have common and distinct functions during polarity establishment and maintenance of cell polarity in N. crassa.

  17. Sistema Solar

    OpenAIRE

    Federación de Asociaciones de Astronomía Cielo de Comellas

    2004-01-01

    Lección sobre el Sistema Solar. Curso de Astronomía Básica, segunda edición, impartido por los miembros de la Federación de Asociaciones de Astronomía Cielo de Comellas. Casa de la Ciencia, sábados, del 24 de septiembre al 22 de octubre de 2011

  18. PROTAGONISMO DOS ÍNDIOS NORTE-AMERICANOS NOS DESENHOS ANIMADOS DE WALTER LANTZ

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    RAFAEL DUARTE OLIVEIRA VENANCIO

    2011-06-01

    Full Text Available Condemned to a dubious media representation in early cinema through thenovels of the late nineteenth century, the North American Indians never had a high profile infilms of this period. However, an animator, Walter Lantz, has a high degree of characterizationof Native Americans in his work. The aim of this paper is to study the role of Native Americansin these cartoons and analyze the social implications of these choices, using the concepts ofapparatus and formula. Furthermore, the analysis will include a portrayal of the Americanmedia scene before and after Lantz, seeking disruptions and legacies.Condenados a uma duvidosa representação midiática no início do cinema graçasaos romances do final do século XIX, os índios norte-americanos nunca tiveram um grandedestaque nos filmes dessa época. No entanto, um realizador de desenhos animados, WalterLantz, possui um alto grau de caracterização de nativos norte-americanos em seus trabalhos. Oobjetivo do presente artigo é estudar o protagonismo dos índios norte-americanos nessesdesenhos animados, e analisar as implicações sociais dessas escolhas através do uso dosconceitos de dispositivo e fórmula. Além disso, a análise incluirá a retratação da cenamidiática norte-americana antes e depois de Lantz, buscando rupturas e legados.

  19. [Sistema Educativo

    OpenAIRE

    Silva, Bento Duarte da

    1989-01-01

    A consagração na Lei nº 46/86, mais conhecida por Lei de Bases do Sistema Educativo (LBSE), aprovada em Outubro de 1986, da criação dos departamentos regionais de educação (44º - 2) que podem vir a reunir um amplo leque de competências em todos os sectores educativos , de estruturas a nível local que assegurem a interligação do sistema educativo com a comunidade (43º - 2) atribuindo-se especial responsabilidade aos municipios (63º - 2), só por si justifica e torna premente a realização deste ...

  20. Primer registro de oso negro americano (Ursus americanus) para el estado de Hidalgo, México

    OpenAIRE

    Alberto Enrique Rojas-Martínez; Luis Antonio Juárez-Casillas

    2013-01-01

    Se documenta el primer registro del oso negro americano (Ursus americanus) para el estado de Hidalgo, México, mismo que representa el punto más sureño de la distribución de esta especie. El ejemplar se encontró muerto cerca del poblado El Aguaje de Cerro Prieto en un lugar conocido como La Joya, municipio de La Misión, en el norte del estado, a una altitud de 1 481 m, probablemente correspondía a un individuo errante proveniente del estado de Querétaro.

  1. Dialogismo: emergência do pensamento latino-americano em comunicação

    OpenAIRE

    Yuji Gushiken

    2008-01-01

    Neste artigo estuda-se a emergência do pensamento latino-americano em comunicação, tendo como foco a proposta dialógica que, na história das teorias da comunicação, se caracteriza como crítica à mass communication research da sociologia americana. Situa a emergência desse pensamento comunicacional na América Latina em meio a condições de subdesenvolvimento econômico e social, analisando-o também como crítica aos difusionismos culturais e comunicacionais do processo de globalização. Histori...

  2. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae)

    OpenAIRE

    Dilma Solange Napp; Ubirajara Ribeiro Martins

    2006-01-01

    Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae). Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae). The following new genus and species, all from Bolivia, are describ...

  3. Cdc25 and Wee1: analogous opposites?

    Directory of Open Access Journals (Sweden)

    Kornbluth Sally

    2007-05-01

    Full Text Available Abstract Movement through the cell cycle is controlled by the temporally and spatially ordered activation of cyclin-dependent kinases paired with their respective cyclin binding partners. Cell cycle events occur in a stepwise fashion and are monitored by molecular surveillance systems to ensure that each cell cycle process is appropriately completed before subsequent events are initiated. Cells prevent entry into mitosis while DNA replication is ongoing, or if DNA is damaged, via checkpoint mechanisms that inhibit the activators and activate the inhibitors of mitosis, Cdc25 and Wee1, respectively. Once DNA replication has been faithfully completed, Cdc2/Cyclin B is swiftly activated for a timely transition from interphase into mitosis. This sharp transition is propagated through both positive and negative feedback loops that impinge upon Cdc25 and Wee1 to ensure that Cdc2/Cyclin B is fully activated. Recent reports from a number of laboratories have revealed a remarkably complex network of kinases and phosphatases that coordinately control Cdc25 and Wee1, thereby precisely regulating the transition into mitosis. Although not all factors that inhibit Cdc25 have been shown to activate Wee1 and vice versa, a number of regulatory modules are clearly shared in common. Thus, studies on either the Cdc25 or Wee1-regulatory arm of the mitotic control pathway should continue to shed light on how both arms are coordinated to smoothly regulate mitotic entry.

  4. Léxico asociado a los recursos naturales americanos en el español de Canarias

    OpenAIRE

    Cáceres-Lorenzo, María Teresa; Salas-Pascual, Marcos

    2010-01-01

    International audience La formación del léxico peculiar de una región está íntimamente ligado a las circunstancias históricas, económicas y sociales del mismo. La relación entre los territorios americanos y las Islas Canarias se inicia desde el mismo momento del descubrimiento hasta nuestros días. Esta relación puede ejemplificarse de manera perfecta en el vocabulario vinculado a los recursos naturales de origen americano que han determinado la agricultura, parte de la industria, e incluso...

  5. Diferencias en magnitud de estado nutricional en escolares chilenos según la referencia CDC y OMS 2005-2008

    Directory of Open Access Journals (Sweden)

    Fabián Vásquez

    2013-02-01

    Full Text Available Introducción: Es necesario realizar nuevas discusiones respecto a la magnitud de los problemas nutricionales diagnosticados, al usar CDC u OMS, frente a la existencia de nuevas definiciones biológicas o estadísticas de obesidad. Objetivo: Comparar la evolución de la prevalencia de estado nutricional en escolares de primero básico, desde el año 2005 al 2008, según CDC y OMS. Métodos: Cohorte retrospectiva, de 140.265 escolares de ambos sexos de primero básico, evaluados entre 2005-2008, cuyos datos antropométricos (peso y talla, se obtuvieron del sistema anual de registro del estado nutricional escolar. Para clasificar el estado nutricional, se utilizaron los patrones CDC y OMS. Resultados: Los promedios de IMC fueron levemente diferentes y menores en la niñas que en los niños, en 2005 y 2006. Durante el 2007 y 2008 el promedio de IMC en las niñas alcanzó la cifra observada en los varones. Hubo mayor prevalencia de bajo peso según OMS (p=0,03, con una tendencia a la disminución en los años posteriores. La prevalencia de normalidad fue mayor según el criterio CDC, con una reducción entre el 2005 y 2007 y un incremento 2008 (p<0,001. Hubo una menor prevalencia de sobrepeso según el criterio CDC (p<0,001, con aumento entre el 2005 y 2007, tanto CDC como OMS. La prevalencia de obesidad fue menor según el criterio OMS, no encontrándose diferencia estadísticamente significativa al comparar con el patrón CDC. Conclusiones: Al comparar ambos patrones, CDC tiende a sobreestimar la normalidad y subestimar el sobrepeso, mientras que en obesidad no se encontraron diferencias significativas.

  6. Retornados e inadaptados: el "Americano" Gallego, entre mito y realidad (1880-1930

    Directory of Open Access Journals (Sweden)

    Núñez Seixas, Xosé M.

    1998-12-01

    Full Text Available During the period of mass migration to America, return migrants, also labelled as "americans" or "indianos", played an important role in Galicia's rural regions as technical innovators and catalysers of the articulation of civil society. This article attemps to have a first look on the topic, by researching into the internal diversity of the whole collectivity of return migrants. It also aims at offering a typology of the social and political agency of return migrants in Galicia in comparative perspective.

    En el período de emigración masiva hacia América, los retornados, también denominados "americanos" o "indianos", jugaron un papel importante como introductores de innovaciones técnicas y como catalizadores de la articulación de la sociedad civil en las zonas rurales de Galicia. Este artículo intenta una aproximación al tema, preguntándose por la diversidad interna del colectivo de retornados y ensayando, en virtud de la comparación con otros ejemplos históricos, una tipología de la agencia social y política de los "americanos" gallegos.

  7. CDC WONDER: AIDS Public Use Data

    Data.gov (United States)

    U.S. Department of Health & Human Services — The AIDS Public Information Data Set (APIDS) for years 1981-2002 on CDC WONDER online database contains counts of AIDS (Acquired Immune Deficiency Syndrome) cases...

  8. CDC STATE System Tobacco Legislation - Smokefree Campus

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Campuses....

  9. CDC STATE System Tobacco Legislation - Preemption

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Preemption. The STATE...

  10. CDC WONDER: Online Tuberculosis Information System (OTIS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Online Tuberculosis Information System (OTIS) on CDC WONDER contains information on verified tuberculosis (TB) cases reported to the Centers for Disease Control...

  11. CDC WONDER: Mortality - Multiple Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Multiple Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2009. Data are based on...

  12. CDC STATE System Tobacco Legislation - Preemption Summary

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Preemption. The STATE...

  13. CDC STATE System Tobacco Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Youth Access. The STATE...

  14. CDC STATE System Tobacco Legislation - Licensure

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation—Licensure. The STATE...

  15. CDC WONDER: Daily Fine Particulate Matter

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Daily Fine Particulate Matter data available on CDC WONDER are geographically aggregated daily measures of fine particulate matter in the outdoor air, spanning...

  16. Zika Spreading Rapidly Through Puerto Rico: CDC

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_159430.html Zika Spreading Rapidly Through Puerto Rico: CDC Possibly hundreds ... 2016 FRIDAY, June 17, 2016 (HealthDay News) -- The Zika virus is spreading fast through Puerto Rico, placing ...

  17. CDC Wonder Vaccine Adverse Event Reporting System

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Vaccine Adverse Event Reporting System (VAERS) online database on CDC WONDER provides counts and percentages of adverse event case reports after vaccination,...

  18. CDC WONDER: Sexually Transmitted Disease (STD) morbidity

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sexually Transmitted Disease (STD) Morbidity online databases on CDC WONDER contain case reports reported from the 50 United States and D.C., Puerto Rico,...

  19. CDC WONDER: Sexually Transmitted Disease (STD) Morbidity

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sexually Transmitted Disease (STD) Morbidity online databases on CDC WONDER contain case reports reported from the 50 United States and D.C., Puerto Rico,...

  20. CDC STATE System Tobacco Legislation - Fire Safety

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Fire-Safety. The STATE...

  1. Obesity Rates Rising Among Women: CDC

    Science.gov (United States)

    ... nlm.nih.gov/medlineplus/news/fullstory_159239.html Obesity Rates Rising Among Women: CDC Though a major ... are another group that continues to struggle with obesity. "Obesity remains a public health concern," said Cynthia ...

  2. Obesity Rates Rising Among Women: CDC

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_159239.html Obesity Rates Rising Among Women: CDC Though a major ... are another group that continues to struggle with obesity. "Obesity remains a public health concern," said Cynthia ...

  3. CDC STATE System Tobacco Legislation - Tax

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation-Tax. The STATE System...

  4. Fast Action Can Prevent Sepsis Death: CDC

    Science.gov (United States)

    ... fullstory_160574.html Fast Action Can Prevent Sepsis Death: CDC Know the signs of extreme response to ... treated long before it causes severe illness or death, U.S. health officials report. Sepsis, or septicemia, occurs ...

  5. CDC WONDER: Mortality - Multiple Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mortality - Multiple Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2006. These data are...

  6. CDC WONDER: Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CDC WONDER Mortality - Underlying Cause of Death online database is a county-level national mortality and population database spanning the years since 1979....

  7. CDC STATE System Tobacco Legislation - Advertising

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2015. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Advertising. The STATE...

  8. CDC Updates Spending Plans to Combat Zika

    Science.gov (United States)

    ... 161541.html CDC Updates Spending Plans to Combat Zika Some goals: prepare states for future outbreaks, and ... dollars to prepare states and cities for future Zika virus outbreaks, and to track the effects of ...

  9. Criterios para un sistema de evaluación de la calidad educativa en Guaviare

    OpenAIRE

    Arias Rodríguez, Julio César

    2013-01-01

    Definir unos criterios que nos permitan acercarnos a la construcción de un sistema de evaluación de la calidad de la educación para el departamento del Guaviare, implica reconocer la evaluación como una acción social que ha estado presente en la historia de la humanidad; ella no nació en el campo de la educación sino en el campo de la industria, y a partir de la década de 1990 es asumida en el contexto Latino Americano como una exigencia de las agencias internacionales (Banco Mundial, Fondo M...

  10. CDC Climat - 2011 Sustainable Development Report

    International Nuclear Information System (INIS)

    CDC Climat is the Caisse des Depots (CDC) subsidiary that is dedicated to combating climate change. Its activities aim to support the transition towards a low resource and low greenhouse gas emission (GHG) economy, through services that are cutting-edge, pro table, and in line with CDC's public policy goals. Through its corporate purpose, CDC Climat embodies the CDC's commitments in the sustainable development field. CDC Climat supports the implementation of public GHG emission reduction policies, primarily through emission trading schemes at the European and international level. Since it was founded in 2010, and throughout 2011, its strategic priorities have consisted in: - developing a long-term policy for investing in carbon credits generated by environmental initiatives, as part of the project mechanisms set up by the Kyoto Protocol, and used in the European Emission Trading Scheme; - supporting the development of its investments in carbon finance operators, like BlueNext, the European carbon exchange, for instance; - broadening the scope of its research into climate economics, which is supported by CDC and available to everyone, in order to serve the public and private players concerned. Its teams have supported French and European governments, international organisations and the United Nations, and various NGOs in their work and thinking on the future of tools for combating climate change. They have specifically contributed reports based on their research and operational feedback. When it was founded, CDC Climat was closely linked to public policies aimed at combating climate change via allowance and carbon trading mechanisms. The difficulties encountered by international negotiations, together with the effects of the economic and financial downturn in Europe, have resulted in a very pronounced fall in the price of carbon assets on these markets since the summer of 2011, with no prospect of recovery for several years. This environment is calling some of the

  11. Regulated degradation of the APC coactivator Cdc20

    Directory of Open Access Journals (Sweden)

    Robbins Jonathan A

    2010-09-01

    Full Text Available Abstract Background Cdc20 is a highly conserved activator of the anaphase-promoting complex (APC, promoting cell-cycle-regulated ubiquitination and proteolysis of a number of critical cell-cycle-regulatory targets including securin and mitotic cyclins. APC-Cdc20 activity is tightly regulated, and this regulation is likely important for accurate cell cycle control. One significant component of Cdc20 regulation is thought to be Cdc20 proteolysis. However, published literature suggests different mechanisms and requirements for Cdc20 proteolysis. The degree to which Cdc20 proteolysis is cell-cycle regulated, the dependence of Cdc20 proteolysis on Cdc20 destruction boxes (recognition sequences for APC-mediated ubiqutination, either by Cdc20 or by the related Cdh1 APC activator, and the need for APC itself for Cdc20 proteolysis all have been disputed to varying extents. In animals, Cdc20 proteolysis is thought to be mediated by Cdh1, contributing an intrinsic order of APC activation by Cdc20 and then by Cdh1. One report suggests a Cdh1 requirement for Cdc20 proteolysis in budding yeast; this idea has not been tested further. Results We characterized Cdc20 proteolysis using Cdc20 expressed from its endogenous locus; previous studies generally employed strongly overexpressed Cdc20, which can cause significant artifacts. We analyzed Cdc20 proteolysis with or without mutations in previously identified destruction box sequences, using varying methods of cell cycle synchronization, and in the presence or absence of Cdh1. Cdc20 instability is only partially dependent on destruction boxes. A much stronger dependence on Cdh1 for Cdc20 proteolysis was observed, but Cdh1-independent proteolysis was also clearly observed. Cdc20 proteolysis independent of both destruction boxes and Cdh1 was especially detectable around the G1/S transition; Cdh1-dependent proteolysis was most notable in late mitosis and G1. Conclusions Cdc20 proteolysis is under complex control

  12. A censura aos filmes ibero-americanos na governação de Marcello Caetano

    OpenAIRE

    Morais, Ana Bela

    2014-01-01

    Através do estudo dos processos de censura ao filmes hispano-americanos em Portugal durante os anos de governação de Marcello Caetano (finais de 1968-1974), pretendo investigar os critérios da Comissão de Censura em relação ao modo como eram censurados esses filmes. O presente trabalho apoia-se no estudo dos arquivos do Secretariado Nacional da Informação e Turismo. A informação produzida pela Comissão de Exame e Classificação de Espectáculos, durante o Estado Novo, está concentrada neste esp...

  13. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Dilma Solange Napp

    2006-01-01

    Full Text Available Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae. Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae. The following new genus and species, all from Bolivia, are described in Compsocerini: Ecoporanga gen. nov., type species E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., from Santa Cruz, and Ethemon iuba sp. nov. from Cochabamba.

  14. The role of CDC42 in cancer%CDC42与癌症

    Institute of Scientific and Technical Information of China (English)

    徐永茹; 徐平; 徐锋; 李湘萍

    2016-01-01

    Cell division cycle 42 (CDC42)is a member of Rho guanosine triphosphatase (GTPase) family,which plays an important role in cell proliferation , cell migration and cell transformation .The activity of CDC42 can be regulated by guanine nucleotide exchange factors (GEFs),GTPase activating proteins (GAPs) and guanine nucleotide-dissociation inhib-itors (GDIs).Recently,CDC42 has been reported as abnormally expressed in many human cancers ,suggesting that CDC42 performs complex functions in tumorigenesis .Therefore,this paper aims to shed light on the functions of CDC 42 in cancers in terms of the alteration of CDC42 activity,CDC42 regulators,as well as its effectors.%细胞分裂周期蛋白42(cell division cycle 42,CDC42)是Rho蛋白鸟苷三磷酸酶(guanosine triphosphatase, GTPase)家族成员之一,在细胞增殖、迁移、转化等过程发挥重要作用。与大多数GTPase一样,CDC42的活性受到鸟嘌呤核苷酸转换因子( guanine nucleotide exchange factors , GEF )、GTP 酶激活蛋白( GTPase activating proteins , GAP)和鸟苷酸解离抑制因子( guanine nucleotide-dissociation inhibitors ,GDI)的调控。目前研究表明,CDC42在大多数人癌症组织中表达异常,对癌症的发生发展具有复杂而重要的调控作用。该文就CDC42自身活性变化、CDC42调节因子的变化及其下游效应因子的变化在癌症中的作用进行综述,旨在深入理解CDC42在人类癌症中的作用机制。

  15. Cdc42-mediated tubulogenesis controls cell specification

    DEFF Research Database (Denmark)

    Kesavan, Gokul; Sand, Fredrik Wolfhagen; Greiner, Thomas Uwe;

    2009-01-01

    Understanding how cells polarize and coordinate tubulogenesis during organ formation is a central question in biology. Tubulogenesis often coincides with cell-lineage specification during organ development. Hence, an elementary question is whether these two processes are independently controlled......, or whether proper cell specification depends on formation of tubes. To address these fundamental questions, we have studied the functional role of Cdc42 in pancreatic tubulogenesis. We present evidence that Cdc42 is essential for tube formation, specifically for initiating microlumen formation and later...... for maintaining apical cell polarity. Finally, we show that Cdc42 controls cell specification non-cell-autonomously by providing the correct microenvironment for proper control of cell-fate choices of multipotent progenitors. For a video summary of this article, see the PaperFlick file with the Supplemental Data...

  16. January 21, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-01-21

    The latest numbers from CDC show more than 20,000 people in West Africa have been sick with Ebola, and more than 8,000 have died. As Dr. Tom Frieden explains, some areas may be starting to see a decrease in cases, but that does not mean the fight is over. CDC will continue its work in West Africa until we get to zero new cases.  Created: 1/21/2015 by CDC’s Office of the Associate Director for Communication.   Date Released: 1/21/2015.

  17. CDC Best Practices for Comprehensive Tobacco Control Programs - 2007

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). Best Practices for Comprehensive Tobacco Control Programs. Funding. CDC's Best Practices for Comprehensive Tobacco...

  18. CDC Best Practices for Comprehensive Tobacco Control Programs - 2014

    Data.gov (United States)

    U.S. Department of Health & Human Services — Centers for Disease Control and Prevention (CDC). Best Practices for Comprehensive Tobacco Control Programs. Funding. CDC's Best Practices for Comprehensive Tobacco...

  19. Characterization and Expression of Mouse Cdc50c during Spermatogenesis

    Institute of Scientific and Technical Information of China (English)

    Peng XU; Xiaoyan DING

    2007-01-01

    Cdc50p is a transmembrane protein required for polarized growth in Saccharomyces cerevisiae.The knowledge on physiological functions of its mammalian homologs, however, is limited. Using database analysis, we identified one mouse testis expressed sequence tag, named Cdc50c, encoding a previously uncharacterized homolog of Cdc50p. Similar to yeast Cdc50p, the putative Cdc50c protein contains three transmembrane spanning regions. Its orthologs are present in many species such as fish, avian and human,suggesting its evolutionary conservation. In multitissue reverse transcription-polymerase chain reaction analyses the mRNA for Cdc50c was predominately detected in testis. The onset of the gene expression coincides with the first appearance of spermatocytes during testicular development. In situ hybridization analyses revealed that Cdc50c mRNA localized in pachytene spermatocytes and round and elongated spermatids. Our data suggest that Cdc50c might play important roles during spermatogenesis.

  20. December 17, 2014 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2014-12-17

    This podcast provides an update on the Ebola response, as of December 17, 2014. It briefly describes CDC's "Operation Care Package.".  Created: 12/17/2014 by CDC’s Office of the Associate Director for Communication.   Date Released: 12/17/2014.

  1. June 2, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-06-02

    In this podcast, CDC's Dr. Jane Seward discusses the Ebola vaccine trial known as STRIVE, which began in April 2015.  Created: 6/2/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 6/2/2015.

  2. 13 CFR 120.851 - CDC ethical requirements.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false CDC ethical requirements. 120.851 Section 120.851 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Development Company Loan Program (504) Other Cdc Requirements § 120.851 CDC ethical requirements. CDCs and...

  3. Cdc48: A Swiss Army Knife of Cell Biology

    Directory of Open Access Journals (Sweden)

    Guem Hee Baek

    2013-01-01

    Full Text Available Cdc48 (also called VCP and p97 is an abundant protein that plays essential regulatory functions in a broad array of cellular processes. Working with various cofactors, Cdc48 utilizes its ATPase activity to promote the assembly and disassembly of protein complexes. Here, we review key biological functions and regulation of Cdc48 in ubiquitin-related events. Given the broad employment of Cdc48 in cell biology and its intimate ties to human diseases (e.g., amyotrophic lateral sclerosis, studies of Cdc48 will bring significant insights into the mechanism and function of ubiquitin in health and diseases.

  4. Un puente entre los mundos. El Instituto Ibero-Americano de Berlín

    Directory of Open Access Journals (Sweden)

    Diana von Römer

    2005-10-01

    Full Text Available El presente artículo ofrece al lector una visión de conjunto sobre el Instituto Ibero-Americano (IAI en Berlín. En primer lugar, relata la historia del instituto desde que se fundara en 1930, cuando surgió el modelo de tres pilares: información, investigación e intercambio cultural, hasta la actualidad, en la que éste sigue vigente. A continuación se exponen en detalle las tres funciones principales que cumple el instituto: centro de información - el IAI alberga la mayor biblioteca de Europa especializada en las culturas ibero-americanas - con sus diversos fondos y colecciones; centro de investigación, con el apoyo a la investigación a través de becas, publicaciones y coloquios; y finalmente el centro cultural, con actividades de diversa índole en colaboración con otras instituciones y embajadas, favoreciendo así el diálogo entre Alemania e Iberoamérica, el Caribe, España y Portugal.

  5. Científicos americanos en las cortes constituyentes. La cuestión ultramarina

    Directory of Open Access Journals (Sweden)

    Maldonado Polo, Jose Luis

    2003-04-01

    Full Text Available This article studies the performance in the Spanish Cortes of three well known American Botanists in the constitutional phase previous to Independence of their countries of origin: Francisco Antonio Zea from New Granada, José María Lequerica from Quito, and Pablo de la Llave from New Spain. Their political motivations and their positioning vis à vis the Parliamentary debates that took place in regard to problems affecting the American colonies are analyzed through two main sources: the Actas de Bayona and the Diario de Sesiones.

    Con este estudio pretendemos contribuir al mayor conocimiento sobre un capítulo poco estudiado de la historia de la ciencia hispanoamericana. Lo haremos a través del seguimiento de las actuaciones como diputados en las Cortes españolas de tres destacados botánicos americanos en las etapas constitucionales anteriores a la emancipación de sus países de origen: el neogranadino Francisco Antonio Zea, el quiteño José Mejía Lequerica y el novohispano Pablo de la Llave. Damos a conocer los impulsos políticos que les motivaron y sus posiciones ante las cuestiones más relevantes debatidas en el parlamento en relación con los problemas que afectaban a las colonias americanas. Las Actas de Bayona y el Diario de Sesiones de las Cortes constituyen las fuentes principales del presente trabajo.

  6. El viaje americano del botánico Carl Friedrich Eduard Otto (1838-1841

    Directory of Open Access Journals (Sweden)

    Rebok, Sandra

    2008-12-01

    Full Text Available The German naturalist Carl Friedrich Eduard Otto travelled during the 1838-1841 period through the West Indies and the American continent looking for botanical specimen and made a study on the social customs of the inhabitants. He went to Cuba, the United States of America and Venezuela. This work contains a further description of the places he visited and the itineraries he followed. The alive plants he harvested, were sent to the Botanical Garden in Berlin while the dry plants were sent to the Royal Herbarium. This study focuses on Otto and Humbolt’s different criteria regarding the slavery and describes Otto’s ideological class-conscious positions.El naturalista alemán Carl Friedrich Eduard Otto viajó entre los años 1838 a 1841 por las Antillas y el Continente americano en busca de especímenes botánicos y para estudiar las costumbres sociales de sus habitantes. Visitó Cuba, Estados Unidos y Venezuela. El trabajo describe los lugares visitados e itinerarios seguidos. Las plantas vivas recolectadas tuvieron como destino el Jardín Botánico de Berlín y las plantas secas el Real Herbario. El estudio señala las diferencias de criterio entre Otto y Humbolt en el tema de la esclavitud y describe las posiciones ideológicas clasistas del primero.

  7. Human Cdc14B promotes progression through mitosis by dephosphorylating Cdc25 and regulating Cdk1/cyclin B activity.

    Directory of Open Access Journals (Sweden)

    Indra Tumurbaatar

    Full Text Available Entry into and progression through mitosis depends on phosphorylation and dephosphorylation of key substrates. In yeast, the nucleolar phosphatase Cdc14 is pivotal for exit from mitosis counteracting Cdk1-dependent phosphorylations. Whether hCdc14B, the human homolog of yeast Cdc14, plays a similar function in mitosis is not yet known. Here we show that hCdc14B serves a critical role in regulating progression through mitosis, which is distinct from hCdc14A. Unscheduled overexpression of hCdc14B delays activation of two master regulators of mitosis, Cdc25 and Cdk1, and slows down entry into mitosis. Depletion of hCdc14B by RNAi prevents timely inactivation of Cdk1/cyclin B and dephosphorylation of Cdc25, leading to severe mitotic defects, such as delay of metaphase/anaphase transition, lagging chromosomes, multipolar spindles and binucleation. The results demonstrate that hCdc14B-dependent modulation of Cdc25 phosphatase and Cdk1/cyclin B activity is tightly linked to correct chromosome segregation and bipolar spindle formation, processes that are required for proper progression through mitosis and maintenance of genomic stability.

  8. ECIS - Adaptation to CDC CYBER system

    International Nuclear Information System (INIS)

    The implantation of ECIS computer code, elaborated for BOURROUGHS 6700 computer of USP (Universidade de Sao Paulo), in the CDC CYBER 170/730 computer is presented. The ECIS code calculates cross section by coupled channel method. Some problems were calculated to verify the compactibility of results obtained from BOURROUGHS 6700 version with the version. The problems calculated by new version and the carried out modifications, are described (M.C.K.)

  9. February 20, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-02-20

    A strategy to rapidly identify and respond to cases of Ebola in remote areas in Liberia led to a drastic reduction in Ebola cases and cut the duration of these cluster outbreaks in half. Learn how CDC responders and their partners trekked through the jungle in their efforts to quickly stop the spread of the virus.  Created: 2/20/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 2/20/2015.

  10. September 24, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-09-24

    In this podcast, CDC medical officer Alyson Goodman describes how CDC’s Children’s Health Team worked with partners to help U.S. hospitals prepare for a potential case of Ebola in a child.  Created: 9/24/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 9/24/2015.

  11. CDC Vital Signs-Heroin Epidemic

    Centers for Disease Control (CDC) Podcasts

    2015-07-07

    This podcast is based on the July 2015 CDC Vital Signs report. Heroin use and heroin-related overdose deaths are increasing. Most people are using it with other drugs, especially prescription opioid painkillers. Learn what can be done to prevent and treat the problem.  Created: 7/7/2015 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 7/7/2015.

  12. CDC Vital Signs-Preventing Melanoma

    Centers for Disease Control (CDC) Podcasts

    2015-06-02

    This podcast is based on the June 2015 CDC Vital Signs report. Skin cancer is the most common form of cancer in the U.S. In 2011, there were more than 65,000 cases of melanoma, the most deadly form of skin cancer. Learn how everyone can help prevent skin cancer.  Created: 6/2/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 6/2/2015.

  13. Desenvolvimento de Sistemas Gastroretentivos

    OpenAIRE

    Bettencourt, Catarina Maria Silveira

    2012-01-01

    Trabalho apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas Os sistemas gastroretentivos são formas farmacêuticas de libertação modificada desenhadas com o intuito de prolongarem o seu tempo de residência no trato gastrointestinal superior. Estes sistemas surgiram para preencher lacunas apresentadas pelos sistemas convencionais de libertação modificada. Os sistemas de libertação controlada convencionais não são des...

  14. Dialogismo: emergência do pensamento latino-americano em comunicação

    Directory of Open Access Journals (Sweden)

    Yuji Gushiken

    2008-09-01

    Full Text Available Neste artigo estuda-se a emergência do pensamento latino-americano em comunicação, tendo como foco a proposta dialógica que, na história das teorias da comunicação, se caracteriza como crítica à mass communication research da sociologia americana. Situa a emergência desse pensamento comunicacional na América Latina em meio a condições de subdesenvolvimento econômico e social, analisando-o também como crítica aos difusionismos culturais e comunicacionais do processo de globalização. Historicamente, o dialogismo atualiza os processos de inovação teórica que reavaliaram a recepção como mediadora e produtora de sentidos nos estudos em comunicação. Palavras-chave: Comunicação; dialogismo; América Latina. ABSTRACT In this article, latin-american thought emergence in communication is studied focusing on the dialogue proposal which, in the history of communication theories, is characterised as a critique of mass communication research in American sociology. Latin-american thought emergence in communication is situated within conditions of economic and socialunderdevelopment being analysed also as a critique of the idea of cultural and communication dispersion of the globalisation process. Historically, dialogism updates the processes of theory innovation which reassesses reception as a mediator and producer of meaning in the studies of communication. Keywords: Communication; dialogism; Latin America.

  15. El imaginario flamenco americano: Aura y Kitsch en la escena transnacional

    Directory of Open Access Journals (Sweden)

    Asensio, Susana

    2004-12-01

    Full Text Available The continuous transformations suffered by flamenco have always been related to its interactions with many other traditions. What once was a local experience, although hybrid from its origins, was transformed into a chameleonic musical phenomenon able to produce new development while conserving a characteristic personality. The transnational flamenco progress started around the end of 19th century. It is considered that its trips to America, especially those to the United States, were the most relevant to the phenomenon. However, flamenco artists developed their careers detached from its socio-cultural environment —and even from American cultures— in the new settings. Their isolation was twofold and favored specific ways to perform and experience flamenco. This new expansion gave transnational flamenco a distinctive and contemporary Kitsch touch.

    Las continuas transformaciones sufridas por el flamenco han estado siempre relacionadas con su interacción con innumerables tradiciones. Lo que una vez fue un fenómeno local, aunque híbrido desde su origen, se convierte en un fenómeno musical camaleónico, capaz de producir fenómenos nuevos al tiempo que sigue manteniendo su personalidad distintiva. El movimiento transnacional del flamenco comenzó ya en el siglo XIX, aunque podemos considerar que su viaje a los Estados Unidos es el que más relevancia ha tenido. Allí, sin embargo, los artistas desarrollaron sus carreras no sólo aislados del entorno sociocultural flamenco, sino también de la cultura popular americana. Su aislamiento fue doble y así los artistas americanos desarrollaron maneras específicas de interpretar y experienciar el flamenco representativas del Kitsch más contemporáneo.

  16. SISTEMA INTERAMERICANO, COOPERAÇÃO E A COMPETIÇÃO INTRAREGIONAL

    Directory of Open Access Journals (Sweden)

    Sabrina Evangelista Medeiros

    2014-12-01

    Full Text Available Este texto tem como propósito a análise do sistema inter-americano e da Organização dos Estados Americanos por meio de sua história e componentes relacionadas às tratativas e organicidade do sistema. Para isso, esta abordagem tem como base a sua evolução institucional, os diferentes usos conceituais e de propósito, as principais fases deste sistema e, em consequência, a reputação associada a ele em que pese os marcos regulatórios e compromissos assumidos no âmbito da OEA e fora dele. A hipótese é de que, a OEA teve constante evolução institucional por conta da transição de objetivos que sofreu entre o fim da Guerra Fria e o século XXI e que, nesta conjuntura e como forma de adaptar-se às outras frentes regionais potencialmente competitivas, volta-se para a questão da segurança individual e direitos humanos criando um guarda-chuva em que cabem, de maneira complementar, as demais iniciativas setorialmente organizadas, como as de finalidade energética, comercial ou de defesa. Por último, que a configuração do setor de defesa nestes mesmos marcos regulatórios criados ao longo destes anos apresenta-se pela fusão de fatores multidimensionais, que vão desde a segurança civil às fronteiras, com poucos aspectos tradicionais de defesa, realocando o seu papel na região

  17. Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication.

    Science.gov (United States)

    Chang, FuJung; Riera, Alberto; Evrin, Cecile; Sun, Jingchuan; Li, Huilin; Speck, Christian; Weinreich, Michael

    2015-08-25

    To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that although Cdc6 ATP hydrolysis is essential to initiate DNA replication, it is not essential for MCM loading. Using purified proteins, an ATPase-defective Cdc6 mutant 'Cdc6-E224Q' promoted MCM loading on DNA. Cdc6-E224Q also promoted MCM binding at origins in vivo but cells remained blocked in G1-phase. If after loading MCM, Cdc6-E224Q was degraded, cells entered an apparently normal S-phase and replicated DNA, a phenotype seen with two additional Cdc6 ATPase-defective mutants. Cdc6 ATP hydrolysis is therefore required for Cdc6 disengagement from the pre-RC after helicase loading to advance subsequent steps in helicase activation in vivo.

  18. Presencia de visón americano (Neovison vison, Schreber 1777 en Chiloé, Chile: ¿inicio de una invasión biológica?

    Directory of Open Access Journals (Sweden)

    G. Vergara

    2015-01-01

    Full Text Available El visón americano fue introducido en Chile en la década de 1930 y actualmente presenta una amplia distribución geográfica. Sin embargo, no se han reportado poblaciones establecidas en muchos sistemas insulares. El día 6 de febrero de 2013, fue hallado un individuo juvenil en Chacao, Chiloé, Chile. El Canal de Chacao representa la única barrera biogeográfica a la isla de Chiloé, con una distancia mínima al continente de 2 km. Se presume que el individuo sorteó esta barrera por sus propios medios, ingresó en una embarcación o mediante un tercero. La introducción de un mamífero carnívoro podría generar severas consecuencias para los ecosistemas de la isla. Los planes preventivos con la comunidad e instituciones públicas y privadas representan una herramienta esencial para el manejo de esta especie y evitar tales impactos en Chiloé.

  19. El Sistema's Open Secrets

    Science.gov (United States)

    Booth, Eric

    2011-01-01

    In this article, the author talks about Venezuela's national youth orchestra program called El Sistema whose attributes offer a direct challenge to traditional Western music practices. As U.S. classical music--and all "high arts"--struggle to find relevance to more than the small "arts club" percentage of the U.S. populace, El Sistema proposes…

  20. O DIREITO FUNDAMENTAL AO MEIO AMBIENTE NO NOVO CONSTITUCIONALISMO LATINO-AMERICANO: BOLÍVIA E EQUADOR

    Directory of Open Access Journals (Sweden)

    Ana Cristina Willemann

    2013-11-01

    constitucional que vem se desenvolvendo nos países latinos; e (ii abordar o direito ao meio ambiente na nova visão do constitucionalismo latino-americano; e analisar como o respeito a Pacha Mama, Madre Tierra, impõe o direito fundamental à água e aos outros seres vivos. A metodologia utilizada é a revisão bibliográfica por meio de leitura e análise comparada dos textos dos autores mencionados na bibliografia deste artigo.

  1. Analysis list: Cdc73 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Cdc73 Muscle + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Cdc73.1.ts...v http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Cdc73.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Cd...c73.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Cdc73.Muscle.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Muscle.gml ...

  2. Cdc42 deficiency causes ciliary abnormalities and cystic kidneys.

    Science.gov (United States)

    Choi, Soo Young; Chacon-Heszele, Maria F; Huang, Liwei; McKenna, Sarah; Wilson, F Perry; Zuo, Xiaofeng; Lipschutz, Joshua H

    2013-09-01

    Ciliogenesis and cystogenesis require the exocyst, a conserved eight-protein trafficking complex that traffics ciliary proteins. In culture, the small GTPase Cdc42 co-localizes with the exocyst at primary cilia and interacts with the exocyst component Sec10. The role of Cdc42 in vivo, however, is not well understood. Here, knockdown of cdc42 in zebrafish produced a phenotype similar to sec10 knockdown, including tail curvature, glomerular expansion, and mitogen-activated protein kinase (MAPK) activation, suggesting that cdc42 and sec10 cooperate in ciliogenesis. In addition, cdc42 knockdown led to hydrocephalus and loss of photoreceptor cilia. Furthermore, there was a synergistic genetic interaction between zebrafish cdc42 and sec10, suggesting that cdc42 and sec10 function in the same pathway. Mice lacking Cdc42 specifically in kidney tubular epithelial cells died of renal failure within weeks of birth. Histology revealed cystogenesis in distal tubules and collecting ducts, decreased ciliogenesis in cyst cells, increased tubular cell proliferation, increased apoptosis, increased fibrosis, and led to MAPK activation, all of which are features of polycystic kidney disease, especially nephronophthisis. Taken together, these results suggest that Cdc42 localizes the exocyst to primary cilia, whereupon the exocyst targets and docks vesicles carrying ciliary proteins. Abnormalities in this pathway result in deranged ciliogenesis and polycystic kidney disease.

  3. Cdc20 control of cell fate during prolonged mitotic arrest

    DEFF Research Database (Denmark)

    Nilsson, Jakob

    2011-01-01

    The fate of cells arrested in mitosis by antimitotic compounds is complex but is influenced by competition between pathways promoting cell death and pathways promoting mitotic exit. As components of both of these pathways are regulated by Cdc20-dependent degradation, I hypothesize that variations...... in Cdc20 protein levels, rather than mutations in checkpoint genes, could affect cell fate during prolonged mitotic arrest. This hypothesis is supported by experiments where manipulation of Cdc20 levels affects the response to antimitotic compounds. The observed differences in Cdc20 levels between cell...

  4. Cdc42 deficiency causes ciliary abnormalities and cystic kidneys.

    Science.gov (United States)

    Choi, Soo Young; Chacon-Heszele, Maria F; Huang, Liwei; McKenna, Sarah; Wilson, F Perry; Zuo, Xiaofeng; Lipschutz, Joshua H

    2013-09-01

    Ciliogenesis and cystogenesis require the exocyst, a conserved eight-protein trafficking complex that traffics ciliary proteins. In culture, the small GTPase Cdc42 co-localizes with the exocyst at primary cilia and interacts with the exocyst component Sec10. The role of Cdc42 in vivo, however, is not well understood. Here, knockdown of cdc42 in zebrafish produced a phenotype similar to sec10 knockdown, including tail curvature, glomerular expansion, and mitogen-activated protein kinase (MAPK) activation, suggesting that cdc42 and sec10 cooperate in ciliogenesis. In addition, cdc42 knockdown led to hydrocephalus and loss of photoreceptor cilia. Furthermore, there was a synergistic genetic interaction between zebrafish cdc42 and sec10, suggesting that cdc42 and sec10 function in the same pathway. Mice lacking Cdc42 specifically in kidney tubular epithelial cells died of renal failure within weeks of birth. Histology revealed cystogenesis in distal tubules and collecting ducts, decreased ciliogenesis in cyst cells, increased tubular cell proliferation, increased apoptosis, increased fibrosis, and led to MAPK activation, all of which are features of polycystic kidney disease, especially nephronophthisis. Taken together, these results suggest that Cdc42 localizes the exocyst to primary cilia, whereupon the exocyst targets and docks vesicles carrying ciliary proteins. Abnormalities in this pathway result in deranged ciliogenesis and polycystic kidney disease. PMID:23766535

  5. CDC Vital Signs-Hispanic Health

    Centers for Disease Control (CDC) Podcasts

    2015-05-05

    This podcast is based on the May 2015 CDC Vital Signs report. About one in six people living in the U.S. are Hispanic. The two leading causes of death in this group are heart disease and cancer, accounting for two out of five deaths. Unfortunately, many Hispanics face considerable barriers to getting high quality health care, including language and low income. Learn what can be done to reduce the barriers.  Created: 5/5/2015 by Office of Minority Health & Health Equity (OMHHE).   Date Released: 5/5/2015.

  6. April 28, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-04-28

    In any disease outbreak, misinformation, a lack of understanding, and fear can lead to unfortunate side effects, like stigma. Stigma presents a challenge for communities during a time when they need to be strong to fight the disease. In this podcast, Molly Gaines-McCollom, CDC Health Communication Specialist, discusses the impact of stigma in the current Ebola outbreak and why it’s so important to fight it.  Created: 4/28/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 4/28/2015.

  7. March 19, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-03-19

    The Ebola outbreak has caused many people to ask whether animals, or specifically pets, are at risk of getting and spreading Ebola in the United States. In this podcast, Drs. Casey Barton Behravesh and Heather Bair-Brake, veterinarians at CDC, discuss how Ebola can affect animals, whether pets in the U.S. are at risk, and what people being monitored for Ebola should do if they have pets at home.  Created: 3/19/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 3/19/2015.

  8. January 8, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-01-08

    CDC scientists, recently returned from Guinea, recount their infection control training course for Guinean healthcare workers who work in health facilities other than Ebola Treatment Units. These workers treat anyone from pregnant women to suspected Ebola cases. It’s critical they be able to recognize and properly treat Ebola patients, not only to protect their own health, but that of their patients.  Created: 1/8/2015 by CDC’s Office of the Associate Director for Communication.   Date Released: 1/8/2015.

  9. CDC Vital Signs-Heart Age

    Centers for Disease Control (CDC) Podcasts

    2015-09-01

    This podcast is based on the September 2015 CDC Vital Signs report. Your heart age is the age of your heart and blood vessels as a result of your risk factors for heart attack and stroke. If you smoke or have high blood pressure, your heart age will be much higher than your actual age. Learn what you can do to lower your heart age and keep it low.  Created: 9/1/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/1/2015.

  10. Perseguir al SRAG: CDC en acción (Stalking SARS: CDC at Work)

    Centers for Disease Control (CDC) Podcasts

    2013-04-29

    En este podcast los niños de Kidtastics hablan sobre el brote del SRAS y cómo trabajaron los CDC para resolver el misterio.  Created: 4/29/2013 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 8/10/2016.

  11. Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes

    International Nuclear Information System (INIS)

    CDC25 phosphatases are important regulators of the cell cycle. Their abnormal expression detected in a number of tumors implies that their dysregulation is involved in malignant transformation. However, the role of CDC25s in vulvar cancer is still unknown. To shed light on their roles in the pathogenesis and to clarify their prognostic values, expression of CDC25A, CDC25B and CDC25C in a large series of vulvar squamous cell carcinomas were examined. Expression of CDC25A, CDC25B, CDC25C and phosphorylated (phospho)-CDC25C (Ser216) were examined in 300 vulvar carcinomas using immunohistochemistry. Western blot analysis was utilized to demonstrate CDC25s expression in vulvar cancer cell lines. Kinase and phosphatase assays were performed to exclude cross reactivity among CDC25s isoform antibodies. High nuclear CDC25A and CDC25B expression were observed in 51% and 16% of the vulvar carcinomas, respectively, whereas high cytoplasmic CDC25C expression was seen in 63% of the cases. In cytoplasm, nucleus and cytoplasm/nucleus high phospho-CDC25C (Ser216) expression was identified in 50%, 70% and 77% of the carcinomas, respectively. High expression of CDC25s correlated significantly with malignant features, including poor differentiation and infiltration of vessel for CDC25B, high FIGO stage, presence of lymph node metastases, large tumor diameter, poor differentiation for CDC25C and high FIGO stage, large tumor diameter, deep invasion and poor differentiation for phospho-CDC25C (Ser216). In univariate analysis, high expression of phospho-CDC25C (Ser216) was correlated with poor disease-specific survival (p = 0.04). However, such an association was annulled in multivariate analysis. Our results suggest that CDC25C and phospho-CDC25C (Ser216) play a crucial role and CDC25B a minor role in the pathogenesis and/or progression of vulvar carcinomas. CDC25B, CDC25C and phospho-CDC25C (Ser216) were associated with malignant features and aggressive cancer phenotypes. However, the

  12. Disfagia orofaríngea neurogênica: análise de protocolos de videofluoroscopia brasileiros e norte-americanos

    Directory of Open Access Journals (Sweden)

    Patrícia Dorotéia de Resende

    2015-10-01

    Full Text Available Resumo: Este artigo tem por objetivo analisar e comparar o uso de protocolos brasileiros e norte-americanos para videofluoroscopia da deglutição em pacientes com histórico de acidente vascular encefálico. Trata-se de uma revisão bibliográfica de artigos brasileiros e norte-americanos. Foram selecionados artigos com descrição de procedimentos para videofluoroscopia da deglutição em sujeitos que sofreram acidente vascular encefálico, publicados entre 2003 e 2013. Os procedimentos descritos para realização de videofluoroscopias foram analisados de forma quantitativa e qualitativa. Consideraram-se as variáveis: posicionamento para o exame, consistências e quantidades de oferta de bolo, tipos de utensílios e utilização de protocolos Foram encontrados 16 artigos que atenderam aos critérios supracitados, sendo nove (56,25% norte-americanos e sete (43,75% brasileiros. Observou-se que quatro dos estudos nacionais (57,14% não relatam as quantidades oferecidas, enquanto todos os artigos norte-americanos detalharam as quantidades, que variaram entre 2 e 15 mL; os tipos de alimento e suas consistências foram detalhadas em todos os estudos nacionais e norte-americanos, porém os nacionais o fizeram de forma genérica, sem identificação dos alimentos. Quanto à posição do paciente durante o exame, 57,14% dos artigos nacionais não relataram e todos os artigos norte-americanos o fizeram. Os utensílios para oferta do bolo são citados em 55,55% dos artigos norte-americanos e em apenas 14,28 % dos nacionais. Quanto à utilização de protocolos para avaliação 33% dos estudos norte-americanos não os citam, comparados aos 71,42% dos nacionais. Observou-se a necessidade de padronização dos procedimentos e terminologias utilizadas na videofluoroscopia da deglutição, de modo a facilitar a intercompreensão e reprodutibilidade dos estudos.

  13. Um exemplo de inversão do domínio ideológico norte-americano no Brasil

    OpenAIRE

    Carla Damião

    2014-01-01

    Propomos discutir a relação de domínio ideológico-cultural norte-americano sobre o Brasil, tendo em vista exemplos documentados. Trata-se da chamada “política de boa vizinhança” [Good Neighbor Policy], com início em 1933 até o final da Segunda Guerra, notadamente durante o governo Getúlio Vargas no Brasil e o governo Franklin Roosevelt nos Estados Unidos. A necessidade de propaganda política passava pela “sedução” cultural da potência estadunidense sobre seus vizinhos latino-americanos. Neste...

  14. Éxito de eclosión del cocodrilo americano (Crocodylus acutus) y la tortuga golfina (Lepidochelys olivacea) en Puerto Vallarta, Jalisco, México

    OpenAIRE

    Fabio Germán Cupul-Magaña; Oscar Saúl Aranda-Mena

    2005-01-01

    Se presentan resultados históricos del éxito de eclosión para la tortuga golfina (2001- 2004) y para el cocodrilo americano (2004-2005) en la región costera de Puerto Vallarta, Jalisco, México. Se destaca un aumento en el éxito de eclosión para la tortuga golfina a la largo del período observado, producto posiblemente de las campañas de protección. Por otro lado, el estero Boca Negra es considerado como una zona importante para la reproducción del cocodrilo americano.

  15. 13 CFR 120.823 - CDC Board of Directors.

    Science.gov (United States)

    2010-01-01

    ... the CDC manager must possess commercial lending experience. The Board must meet at least quarterly and shall be responsible for CDC staff decisions and actions. A quorum shall require at least 5 Directors... Committee members must include at least one member with commercial lending experience acceptable to SBA....

  16. Estudos latino-americanos sobre melancolia: um transtorno do humor melhor definido para o CID-11 Melancholia in Latin American studies: a distinct mood disorder for the ICD-11

    Directory of Open Access Journals (Sweden)

    Mario F. Juruena

    2011-05-01

    Full Text Available OBJETIVO: A depressão melancólica é um diagnóstico psiquiátrico de história de vida, geralmente com episódios recorrentes. Melancolia é uma síndrome com longa duração e características específicas de psicopatologia, insuficientemente diferenciada de depressão maior por um especificador no DSM-IV e parcialmente descrito nos critérios da Classificação Internacional de Doenças-10ª Edição. Dentro da classificação atual, é frequentemente vista em pacientes gravemente doentes com depressão e transtorno bipolar. No entanto, a melancolia possui uma homogeneidade psicopatológica e biológica distinta na experiência clínica e nos marcadores de testes laboratoriais, e é diferencialmente sensível às intervenções terapêuticas específicas. O objetivo deste estudo é revisar a literatura de artigos publicados por autores latino-americanos sobre a melancolia. MÉTODO: Realizou-se busca de artigos latino-americanos de informações relevantes para a revisão da Classificação Internacional de Doenças-10ª Edição de transtornos mentais e comportamentais em pacientes com depressão melancólica. Foi avaliada a qualidade do design de todos os estudos e realizada uma revisão abrangente sobre o assunto, com o objetivo de considerar a contribuição latino-americana para inclusão da melancolia como uma entidade distinta na futura Classificação Internacional de Doenças-11ª Edição. RESULTADOS E CONCLUSÃO: Os estudos latino-americanos fundamentam o diagnóstico da melancolia com uma psicopatologia e psiconeuroendocrinologia própria que fundamentam ser reconhecida como um transtorno de humor identificável e merecedor de uma atenção específica nos sistemas de classificação, como um transtorno de humor distinto, identificável e especificamente tratável.OBJECTIVE: Melancholic depression is a lifetime diagnosis, typically with recurrent episodes. Melancholia, a syndrome with a long history and distinctive

  17. March 11, 2015 CDC Ebola Response Update

    Centers for Disease Control (CDC) Podcasts

    2015-03-11

    CDC’s Eric Dziuban explains key messages CDC developed with UNICEF and the World Health Organization (WHO) on safe school operations in Guinea, Liberia, and Sierra Leone. After being closed for months due to the Ebola outbreak, schools in these countries are reopening – an important step in helping children and their communities return to normal. The key messages offer a tool that each country’s government can use to develop their own approach on safely reopening schools.  Created: 3/11/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 3/11/2015.

  18. Flavors of the States ‘Saborea los estados’. Proyecto web para la gestión y venta de productos alimenticios americanos

    OpenAIRE

    Aguado García, Carlos

    2013-01-01

    Flavors of the States “Saborea los estados” es un proyecto web para la gestión y venta de productos alimenticios americanos. Es un proyecto en plataforma.net y en un lenguaje C#, utilizando SQL Server e incorporando una pasarela de pago PayPal

  19. Sistema detector d'incendis WSN

    OpenAIRE

    Domínguez López, Emiliano

    2012-01-01

    Sistema detector d'incendis aprofitant una xarxa de sensors sense fils (WSN) mitjançant un sistema encastat. Sistema detector de incendios aprovechando una red de sensores inalámbricos (WSN) mediante un sistema empotrado.

  20. O marxismo indo-americano de Mariátegui e a polêmica com a III Internacional Comunista.

    Directory of Open Access Journals (Sweden)

    María Gabriela Guillén

    2012-05-01

    Full Text Available Partindo da perspectiva marxista indo-americana de José Carlos Mariátegui, o artigo visa a exposição de fatores teóricos e históricos que culminaram na polêmica com a III Internacional Comunista na América Latina. A problemática evidencia a originalidade do pensamento de Mariátegui, cujo eixo central é a interpretação dos fatos históricos, políticos e sociais latino-americanos sob a perspectiva dialética do desenvolvimento desigual e combinado para explicar a dinâmica particular da realidade peruana.

  1. Poder civil y derechos naturales de los indios americanos según Fray Alonso de la Veracruz

    Directory of Open Access Journals (Sweden)

    Manuel Méndez Alonzo

    2013-01-01

    Full Text Available El presente trabajo investiga las tesis sobre el poder civil de Alonso de la Veracruz que buscan incorporar en la comunidad política española a los habitantes autóctonos del Nuevo Mundo, tesis que suelen relacionarse con F. de Vitoria y el tomismo español, y que últimamente son consideradas parte del republicanismo novohispano elaborado desde la periferia americana. Se busca demostrar que su propósito era aplicar una teoría de derechos naturales, sin que ello implique participación política de los indios americanos. Se analiza la postura del fraile frente a la diversidad cultural y la guerra contra los indios.

  2. Auzolagun: Crowdfunding sistema baten garapena

    OpenAIRE

    Mendiola Larringan, Ander

    2013-01-01

    Memoria dokumentuan Auzolagun izeneko proiektuaren nondik-norakoak azaltzen dira. Sistema Informatikoko Ingeniaritza Teknikoko ikasketa amaierako proiektu hau, Talaios kooperatibarekin elkarlanean sortu da, helburu nagusitzat euskal komunitatearentzako crowdfunding sistema bat sortzea delarik.

  3. Subjetividad y sistemas funcionales

    OpenAIRE

    Lechner, Norbert

    2012-01-01

    En la reseña de mi libro «Las sombras del mañana» (Ed. Lom, 2002), Juan Jiménez plantea un problema que merece discusión: «¿La subjetividad es algo importante, relevante, crucial para las personas o las sociedades?» Jiménez afirma que la subjetividad puede ser importante para las personas, pero -como la sociedad no estaría compuesta por personas, sino por sistemas funcionales-, no tendría importancia para el funcionamiento de la sociedad. Apoyándose en Luhmann, supone que los sistemas obedece...

  4. Desenvolupament sistema ERP

    OpenAIRE

    Duran Rosich, Antoni

    2016-01-01

    Introducció al desenvolupament d'un ERP. El projecte està centrat en el programa més extens de Planificació de Recursos Empresarials o ERP (de l'anglès Enterprise Resource Planning) de Microsoft. Aquests integren (o pretenen integrar) totes les dades i processos d'una organització en un sistema unificat. Aquest sistema utilitza múltiples components de programari i maquinari per aconseguir la integració. Un component clau de la majoria d' ERP és l'ús d'una base de dades única per emmagatzem...

  5. Cat Scratch Can Sometimes Lead to Serious Illness: CDC

    Science.gov (United States)

    ... https://medlineplus.gov/news/fullstory_161086.html Cat Scratch Can Sometimes Lead to Serious Illness: CDC But ... Fluffy the cat gets out of sorts and scratches you, it's possible you could get a bacterial ...

  6. CDC STATE System E-Cigarette Legislation - Youth Access

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Youth...

  7. CDC STATE System E-Cigarette Legislation - Tax

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Tax. The...

  8. Smoking Rates Still High in Some Racial Groups, CDC Reports

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_160256.html Smoking Rates Still High in Some Racial Groups, CDC ... lot of progress in getting Americans to stop smoking, some groups still have high smoking rates, a ...

  9. CDC STATE System Tobacco Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Indoor Air....

  10. CDC STATE System E-Cigarette Legislation - Smokefree Indoor Air

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  11. CDC WONDER: Daily Air Temperatures and Heat Index

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Daily Air Temperature and Heat Index data available on CDC WONDER are county-level daily average air temperatures and heat index measures spanning the years...

  12. Smoking Losing Its Cool with Kids, CDC Says

    Science.gov (United States)

    ... finds. The same can't be said of e-cigarettes. Fewer students reported trying cigarettes or cigars between ... still showing a worrisome level of interest in e-cigarettes. The CDC released a survey in June that ...

  13. Southern States Lagging in Tough Smoking Bans, CDC Says

    Science.gov (United States)

    ... 159529.html Southern States Lagging in Tough Smoking Bans, CDC Says Only 6 in 10 Americans covered ... federal government report says. This type of law bans smoking in all indoor areas of workplaces, restaurants ...

  14. CDC STATE System E-Cigarette Legislation - Licensure

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Licensure....

  15. CDC STATE System E-Cigarette Legislation - Preemption

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Preemption....

  16. CDC Ends Zika Travel Advisory for Miami Neighborhood

    Science.gov (United States)

    ... medlineplus.gov/news/fullstory_161032.html CDC Ends Zika Travel Advisory for Miami Neighborhood Pregnant women still ... News) -- U.S. health officials on Monday lifted the Zika virus travel advisory that urged pregnant women to ...

  17. CDC WONDER: Vaccine Adverse Event Reporting System (VAERS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Vaccine Adverse Event Reporting System (VAERS) online database on CDC WONDER provides counts and percentages of adverse event case reports after vaccination, by...

  18. Cdc42 promotes host defenses against fatal infection

    DEFF Research Database (Denmark)

    Lee, Keunwook; Boyd, Kelli L; Parekh, Diptiben V;

    2013-01-01

    The small Rho GTPase, Cdc42, regulates key signaling pathways required for multiple cell functions including maintenance of shape, polarity, proliferation, invasion, migration, differentiation and morphogenesis. As the role of Cdc42-dependent signaling in fibroblasts in vivo is unknown, we...... attempted to specifically delete it in these cells by crossing the Cdc42(fl/fl) mouse with a FSP-1 cre mouse, which is thought to mediate recombination exclusively in fibroblasts. Surprisingly, the FSP-1cre;Cdc42(fl/fl) mice died at 3 weeks of age due to overwhelming suppurative upper airway infections that...... were associated with neutrophilia and lymphopenia. Even though major aberrations in lymphoid tissue development were present in the mice, the principal cause of death was severe migration and killing abnormalities of the neutrophil population resulting in an inability to control infection. We also...

  19. CDC WONDER: Detailed Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Detailed Mortality - Underlying Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2009. Data are...

  20. CDC Warns of Dangers of Plastic Surgery in Dominican Republic

    Science.gov (United States)

    ... a very mutilating infection. They're going for cosmetic surgery, and they will be scarred. It's a terrible ... postoperative infections, particularly ones that are related to cosmetic surgery," Daley said. The CDC report warns about the ...

  1. Synthetic Fentanyl Fueling Surge in Overdose Deaths: CDC

    Science.gov (United States)

    ... https://medlineplus.gov/news/fullstory_160618.html Synthetic Fentanyl Fueling Surge in Overdose Deaths: CDC U.S. Surgeon ... News) -- Deaths from overdoses of the synthetic narcotic fentanyl have surged in recent years, U.S. health officials ...

  2. CDC WONDER: Compressed Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CDC WONDER Mortality - Underlying Cause of Death online database is a county-level national mortality and population database spanning the years since 1979...

  3. CDC STATE System E-Cigarette Legislation - Smokefree Campus

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. E-Cigarette Legislation—Smokefree...

  4. CDC STATE System Tobacco Legislation - Smokefree Indoor Air Summary

    Data.gov (United States)

    U.S. Department of Health & Human Services — 1995-2016. Centers for Disease Control and Prevention (CDC). State Tobacco Activities Tracking and Evaluation (STATE) System. Legislation – Smokefree Indoor Air....

  5. CDC Awards $16M in Fight Against Zika

    Science.gov (United States)

    ... 160203.html CDC Awards $16M in Fight Against Zika Health experts warn pregnant women to avoid downtown ... where mosquitoes are apparently infecting people with the Zika virus. The number of local transmissions of the ...

  6. Heart Surgery Devices May Have Been Contaminated: CDC

    Science.gov (United States)

    ... 161469.html Heart Surgery Devices May Have Been Contaminated: CDC Patients who experience night sweats, fever, weight ... used during open heart surgery may have been contaminated with bacteria that puts patients at risk for ...

  7. The Role of Cdc2 and Other Genes in Meiosis in Schizosaccharomyces Pombe

    OpenAIRE

    Iino, Y.; Hiramine, Y.; Yamamoto, M.

    1995-01-01

    The requirement of the cdc2, cdc13 and cdc25 genes for meiosis in Schizosaccharomyces pombe was investigated using three different conditions to induce meiosis. These genes were known to be required for meiosis II. cdc13 and cdc25 are essential for meiosis I. The cdc2 gene, which is required for the initiation of both mitotic S-phase and M-phase, is essential for premeiotic DNA synthesis and meiosis II. The requirement of cdc2 for meiosis I was unclear. This contrasts with Saccharomyces cerev...

  8. Cdc42 is critical for cartilage development during endochondral ossification.

    Science.gov (United States)

    Suzuki, Wataru; Yamada, Atsushi; Aizawa, Ryo; Suzuki, Dai; Kassai, Hidetoshi; Harada, Takeshi; Nakayama, Mutsuko; Nagahama, Ryo; Maki, Koutaro; Takeda, Shu; Yamamoto, Matsuo; Aiba, Atsu; Baba, Kazuyoshi; Kamijo, Ryutaro

    2015-01-01

    Cdc42 is a widely expressed protein that belongs to the family of Rho GTPases and controls a broad variety of signal transduction pathways in a variety of cell types. To investigate the physiological functions of Cdc42 during cartilage development, we generated chondrocyte-specific inactivated Cdc42 mutant mice (Cdc42(fl/fl); Col2-Cre). The gross morphology of mutant neonates showed shorter limbs and body as compared with the control mice (Cdc42(fl/fl)). Skeletal preparations stained with alcian blue and alizarin red also revealed that the body and the long bone length of the mutants were shorter than those of the control mice. Furthermore, severe defects were found in growth plate chondrocytes in the femur sections of mutant mice, characterized by a reduced proliferating zone height, wider hypertrophic zone, and loss of columnar organization in proliferating chondrocytes. The expression levels of chondrocyte marker genes, such as Col2, Col10, and Mmp13, in mutant mice were decreased as compared with the control mice. Mineralization of trabecular bones in the femur sections was also decreased in the mutants as compared with control mice, whereas osteoid volume was increased. Together these results suggested that chondrocyte proliferation and differentiation in growth plates in the present mutant mice were not normally organized, which contributed to abnormal bone formation. We concluded that Cdc42 is essential for cartilage development during endochondral bone formation. PMID:25343271

  9. El sistema constructivo

    Directory of Open Access Journals (Sweden)

    Leser S., Heinz

    2003-06-01

    Full Text Available Se describe el sistema constructivo de entramado en madera, “plataforma” (platform frame utilizado preferentemente en las construcciones de varios pisos en el campamento minero de Sewell, Chile./The following is a description of the platform frame used in several stories buildings in the mining settlement Sewell, in Chile.

  10. El sistema constructivo

    OpenAIRE

    Leser S., Heinz; Gómez Lerou, Luis; Salomone R., Vanessa

    2003-01-01

    Se describe el sistema constructivo de entramado en madera, “plataforma” (platform frame) utilizado preferentemente en las construcciones de varios pisos en el campamento minero de Sewell, Chile./The following is a description of the platform frame used in several stories buildings in the mining settlement Sewell, in Chile.

  11. Role of AtCDC48 & the AtCDC48 Regulatory Protein Family, PUX, in Plant Cell Morphogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Bednarek, Sebastian, Y.

    2009-11-08

    The long-term objective of this work is to understand the molecular events and mechanisms involved in secretory membrane trafficking and organelle biogenesis, which are crucial for normal plant growth and development. Our studies have suggested a vital role for the cytosolic chaperone Cdc48p/p97 during cytokinesis and cell expansion which are highly dependent upon secretory membrane trafficking. Localization studies have shown that the plant Cdc48p/p97, AtCDC48, and the Arabidopsis ortholog of the ER- and Golgi-associated SNARE, syntaxin 5, (referred to as SYP31) are targeted to the division plane during cytokinesis. In addition, AtCDC48 and SYP31 were shown to interact in vitro and in vivo. To characterize further the function of AtCDC48 and SYP31 we have utilized affinity chromatography and MALDI-MS to identify several plant-specific proteins that interact with SYP31 and/or modulate the activity of AtCDC48 including two UBX (i.e. ubiquitin-like) domain containing proteins, PUX1 and PUX2 (Proteins containing UBX domain). These proteins define a plant protein family consisting of 15 uncharacterized members that we postulate interact with AtCDC48. Biochemical studies have demonstrated that PUX2 is a novel membrane adapter for AtCDC48 that mediates AtCDC48/SYP31 interaction and is likely to control AtCDC48-dependent membrane fusion. In contrast, PUX1 negatively regulates AtCDC48 by inhibiting its ATPase activity and by promoting the disassembly of the active hexamer. These findings provide the first evidence that the assembly and disassembly of the CDC48/p97complex is actually a dynamic process. This new unexpected level of regulation for CDC48/p97 was demonstrated to be critical in vivo as pux1 loss-of-function mutants grow faster than wild-type plants. These studies suggest a role for AtCDC48 in plant cell cycle progression including cytokinesis and/or cell expansion. The proposed studies are designed to: 1) characterize further the localization and function of AtCDC

  12. CDC25A phosphatase controls meiosis I progression in mouse oocytes.

    Science.gov (United States)

    Solc, Petr; Saskova, Adela; Baran, Vladimir; Kubelka, Michal; Schultz, Richard M; Motlik, Jan

    2008-05-01

    CDK1 is a pivotal regulator of resumption of meiosis and meiotic maturation of oocytes. CDC25A/B/C are dual-specificity phosphatases and activate cyclin-dependent kinases (CDKs). Although CDC25C is not essential for either mitotic or meiotic cell cycle regulation, CDC25B is essential for CDK1 activation during resumption of meiosis. Cdc25a -/- mice are embryonic lethal and therefore a role for CDC25A in meiosis is unknown. We report that activation of CDK1 results in a maturation-associated decrease in the amount of CDC25A protein, but not Cdc25a mRNA, such that little CDC25A is present by metaphase I. In addition, expression of exogenous CDC25A overcomes cAMP-mediated maintenance of meiotic arrest. Microinjection of Gfp-Cdc25a and Gpf-Cdc25b mRNAs constructs reveals that CDC25A is exclusively localized to the nucleus prior to nuclear envelope breakdown (NEBD). In contrast, CDC25B localizes to cytoplasm in GV-intact oocytes and translocates to the nucleus shortly before NEBD. Over-expressing GFP-CDC25A, which compensates for the normal maturation-associated decrease in CDC25A, blocks meiotic maturation at MI. This MI block is characterized by defects in chromosome congression and spindle formation and a transient reduction in both CDK1 and MAPK activities. Lastly, RNAi-mediated reduction of CDC25A results in fewer oocytes resuming meiosis and reaching MII. These data demonstrate that CDC25A behaves differently during female meiosis than during mitosis, and moreover, that CDC25A has a function in resumption of meiosis, MI spindle formation and the MI-MII transition. Thus, both CDC25A and CDC25B are critical for meiotic maturation of oocytes.

  13. Ambiente Java para sistemas embedded

    OpenAIRE

    Silva, Valter Filipe Miranda Castelão da

    2002-01-01

    Os sistemas integrados, ou genericamente os sistemas com reduzida capacidade de processamento e pouca memória, são actualmente sistemas economicamente acessíveis e muito divulgados. Os seus sistemas operativos estão, no entanto, ainda bastante dependentes do hardware o que faz que cada aplicação seja especialmente desenvolvida para cada tipo de sistema. Neste documento discute-se a aplicabilidade de uma máquina virtual Java em equipamentos com estas restrições. É proposta a ...

  14. Decreased uv mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae

    Energy Technology Data Exchange (ETDEWEB)

    Prakash, L.; Hinkle, D.; Prakash, S.

    1978-01-01

    A DNA replication mutant of yeast, cdc8, was found to decrease uv-induced reversion of lys2-1, arg4-17, tryl and ural. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following uv irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since uv-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.

  15. As redes de cubanos, norte-americanos e colombianos no tráfico de drogas em miami durante os anos 60

    OpenAIRE

    Sáenz Rovner, Eduardo

    2010-01-01

    Utilizando abundante informação inédita tomada de vários arquivos norte-americanos assim como da imprensa jornalística, especialmente a do sul da Flórida, este artigo estuda o desenvolvimento das redes de traficantes de drogas cubanos, norte-americanos e colombianos em Miami durante os anos 60. O artigo cobre também a entrada massiva de cubanos no sul da Flórida depois da Revolução Cubana, seu desempenho econômico e o clima político na Miami da época. Analisa-se o papel de traficantes cubanos...

  16. 爪形大分子CDC-SO的研究%Claw Type Macromolecule of CDC-SO

    Institute of Scientific and Technical Information of China (English)

    陈玲玲; 龙小柱; 高锦鹏; 王长松; 郭瓦力; 宗学军

    2011-01-01

    选用一缩二乙二醇、柠檬酸、硬脂酸和十八醇为原料,利用酯化反应设计并合成了新的低温流动改性剂:一缩二乙二醇-柠檬酸-硬脂酸-十八醇(CDC-SO).通过单因素试验和正交试验得到了制备CDC-SO的最适宜工艺条件:n(CDC):,n(硬脂酸)为1:1.00;n(CDC-S):n(十八醇)为1:4.0;催化剂用量(以总原料的酸醇总量计)为1.00%;溶剂用量(以总原料的酸醇总量计)为35%;酯化反应时间8 h.将合成的爪形大分子CDC-SO按2.5 mg/g的加剂量添加到沈阳石蜡化工有限公司生产的0#柴油中,冷滤点可降低5℃.%With the diethylene glycol, citric acid, stearic acid and octadeeyl alcohol as raw materials,using the esterification, a new low-temperature flow improver, citric acid-diethylene glycol-citric acidstearic acid-octadecyl alcohol (CDC-SO), was synthesized. Through the single factor and orthogonal experiments, the suitable conditions for preparing CDC-SO were obtained: The mole ratio of CDC to stearic acid was 1: 1.00, the mole ratio of CDC-S to octadecyl alcohol was 1: 4. 0, the dosage of the catalyst was 1.00% based on the total mass of acid and alcohol of the total raw materials, the dosage of the solvent was 35% based on the total mass of acid and alcohol of the total raw materials, the time of the esterification reaction was 8 h. When CDC-SO was added with the dosage of 2.5 mg/g, in 0# diesel oil which was produced by the Shenyang Paraffin Wax Chemical Corporation, thecold filter plugging point could drop 5℃.

  17. Serological surveillance on South American wild canids for Neospora caninum Sorologia para Neospora caninum em canídeos silvestres sul-americanos

    Directory of Open Access Journals (Sweden)

    C.B. Melo

    2002-08-01

    Full Text Available Esse trabalho objetivou pesquisar anticorpos para Neospora caninum em amostras de soro de canídeos silvestres sul-americanos pela técnica de imunofluorescência indireta (IFI. As amostras foram coletadas de 48 lobos-guará (Chrysocyon brachyurus, provenientes de zoológicos e de vida livre e de dois cachorros-do-mato (Cerdocyon thous. Anticorpos para N. caninum não foram detectados nas amostras testadas.

  18. sistema Web con JSP

    Directory of Open Access Journals (Sweden)

    César Viloria Núñez

    2014-01-01

    Full Text Available Este artículo presenta el desarrollo de un sistema de información que permite la adquisición y la administración de información relacionada con los signos vitales como la presión arterial, la frecuencia cardiaca y respiratoria, y la saturación de oxígeno en la sangre de un paciente. La implementación del sistema se basa en una solución Web, permitiendo así que médicos especialistas puedan monitorear a sus pacientes desde cualquier punto conectado a la red en tiempo real y, al mismo tiempo, dar indicaciones críticas al personal médico que se encuentra en el lugar con el paciente.

  19. The dual specificity phosphatase Cdc14B bundles and stabilizes microtubules

    Energy Technology Data Exchange (ETDEWEB)

    Plumley, Hyekyung [ORNL; Liu, Yie [ORNL; Gomez, Marla V [ORNL; Wang, Yisong [ORNL

    2005-01-01

    The Cdc14 dual-specificity phosphatases regulate key events in the eukaryotic cell cycle. However, little is known about the function of mammalian CDC14B family members. Here, we demonstrate that subcellular localization of CDC14B protein is cell cycle regulated. CDC14B can bind, bundle, and stabilize microtubules in vitro independently of its catalytic activity. Basic amino acid residues within the nucleolar targeting domain are important for both retaining CDC14B in the nucleolus and preventing microtubule bundling. Overexpression of CDC14B resulted in the formation of cytoplasmic CDC14B and microtubule bundles in interphase cells. These microtubule bundles were resistant to microtubule depolymerization reagents and enriched in acetylated -tubulin. Expression of cytoplasmic forms of CDC14B impaired microtubule nucleation from the microtubule organization center. CDC14B is thus a novel microtubule-bundling and -stabilizing protein, whose regulated subcellular localization may help modulate spindle and microtubule dynamics in mitosis.

  20. Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea

    Institute of Scientific and Technical Information of China (English)

    ZHENG Wu; CHEN Ji-sheng; ZHENG Shi-qin; LU Guo-dong; WANG Zong-hua

    2006-01-01

    MgCdc42 (Cdc42 in Magnaporthe grisea), with high homology to ScCdc42 (Cdc42 in Saccharomyces cerevisiae), has been demonstrated to involve in the morphogenesis and infection process. To further understand the signaling network,the putative MgCdc42-interacting proteins were analyzed. ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semiquantitative RT-PCR. All retrieved analogs of ScCdc42-interacting proteins from the M.grisea database have conserved domains as those in S. cerevisiae. Expression of their encoding genes increased in MgCdc42CA mutant and decreased in MgCdc42KO mutant. However, MgBem1, Chm1, and MgGic1 in MgCdc42DN mutant had the same expression level as that in the wild type, although MgBem4, MgBoi2, MgCdc24, MgGic2, MgRga1,and Mst20 had decreased expression level, as expected. Overall, it is concluded that there may exist a similar Cdc42 signal pathway in M. grisea as in S. cerevisiae and MgCdc42 plays a key role in the pathway.

  1. Sistemas Silvipastoris Silvipastoral Systems

    Directory of Open Access Journals (Sweden)

    Fernando Salgado Bernardino

    2010-02-01

    Full Text Available

    Apesar das pesquisas com sistemas silvipastoris terem sido iniciadas no final da década de 1970, as informações geradas até hoje não são em grande número. As vantagens advindas de um sistema silvipastoril são inúmeras e devidamente reconhecidas. Pelo fato de ser uma técnica de uso da terra capaz de recuperar ecossistemas alterados pelo mau manejo, estes sistemas, pela integração de atividades agrícolas, pecuárias e silviculturais, passam a representar uma tecnologia que confere maior sustentabilidade que os sistemas tradicionais, nos quais os monocultivos são predominantes. Nos últimos anos, instituições de ensino, pesquisa e extensão do País têm se voltado para o desenvolvimento de tais sistemas. O governo de Minas Gerais, por meio de sua Secretaria de Estado de Agricultura e de órgãos vinculados, vem promovendo a condução de modelos agrossilvipastoris num processo integrado de ocupação do solo, denominado lavoura-pecuária-silvicultura. Apesar dos grandes avanços no conhecimento de culturas anuais e dos componentes arbóreos, principalmente o eucalipto, e de gramíneas forrageiras tolerantes à diminuição da intensidade luminosa, ainda é necessário se comnhecer melhor o manejo do sub-bosque sob os efeitos do pastejo.

     

    doi: 10.4336/2009.pfb.60.77

    Although researches related to silvopastoral systems have been initiated many years ago in Brazil, there are few informations generated until now. The advantages from a silvopastoral system are inumerous and well recognized. By the fact of being a technique of land using capable of recovering several disturbed ecosystems, the silvopastoral systems by the integration of agricultural, cattle, and silvicultural activities represent a technology that can achieve higher sustainability than the traditional systems as the monocultures. Today, research centers in Brazil dedicate great attention to agrossilvopastoral systems. The government of Minas Gerais

  2. "A wild and wondrous ride": CDC field epidemiologists in the east Pakistan smallpox and cholera epidemics of 1958 "Uma louca e maravilhosa jornada": epidemiologistas de campo do CDC nas epidemias de varíola e cólera do Paquistão Oriental em 1958

    Directory of Open Access Journals (Sweden)

    Paul Greenough

    2011-02-01

    desenvolvimento. Langmuir descreveu o episódio como uma "jornada louca e maravilhosa", porém pareceu mais um pesadelo: o departamento de saúde do Paquistão Oriental entrou em colapso; um movimento popular assumiu a vacinação e desperdiçou suprimentos de vacinas; jornalistas hostis questionaram as motivações ocultas dos americanos; e iniciou-se uma rivalidade profissional entre americanos e o epidemiologista britânico Aidan Cockburn. No final da epidemia, em julho de 1958, tinham sido vacinados trinta milhões de bengalis contra varíola, mas outros vinte mil sucumbiram. Esse episódio foi a primeira intervenção sistemática do CDC no exterior.

  3. Uma investigação sobre os co-movimentos na volatilidade dos par bonds latino-americanos

    Directory of Open Access Journals (Sweden)

    Igor A.C. de Morais

    2001-04-01

    Full Text Available Este artigo procura aplicar o método de quase-máxima verossimilhança para estimar a volatilidade estocástica multivariada não-estacionária dos preços de compra dos par bonds de quatro países latino-americanos - México, Brasil, Argentina e Venezuela - no período de 9-8-1994 a 15-9-1999. O objetivo é analisar possíveis movimentos comuns nestas variâncias. Os testes feitos revelam que a volatilidade nos modelos univariados não apresentam inclinação, mas possuem alta persistência. A formulação multivariada relaciona bem os dados, obtendo estimativas consistentes e revelando a existência de um comportamento ao longo do tempo similar entre as volatilidades das quatro séries.This paper uses a quasi-maximum likelihood procedure to estimate the non-stationary stochastic volatility for the par bonds of four Latin American countries: Brazil, Argentina, Mexico and Venezuela. The aim is to investigate the possible presence of co-movements in volatility across countries. The estimation period goes from August 1994 to September 1999, including, therefore, the Asian and Russian crises. The estimated volatility for the univariate model does not show any slope and is highly persistent. The multivariate model gives a good fit to the data and shows that there is common movement.

  4. Boletos sencillos y pasajes redondos. Indígenas y mestizos americanos que visitaron España

    Directory of Open Access Journals (Sweden)

    de Rojas, José Luis

    2009-08-01

    Full Text Available Starting from the pioneer study of Esteban Mira (2001, this paper looks for the travels of to Spain of American Indians, both in documents and chronicles, since the very moment of the Spanish Conquest, mainly those who travelled spontaneously or upon royal orders. The main aim is to show the necessity of deeply analyze these travels, their reasons and circunstances, and search for the eventual constitution of personal webs between the two shores of the Ocean.

    Partiendo del estudio pionero de Esteban Mira (2001, se rastrean en diversos documentos y crónicas los viajes que indígenas americanos realizaron a España, desde poco tiempo después de la conquista, centrándose en los que lo hicieron voluntariamente o por lo que podríamos llamar «razones de estado». A partir de estas noticias se plantea la necesidad de analizar en profundidad estos viajes, sus motivos y avatares y rastrear la posible constitución de redes personales que abarcaran ambas orillas del océano.

  5. Las antiguas colecciones arqueológicas de Yucatán en el Museo Americano de Historia Natural

    Directory of Open Access Journals (Sweden)

    Adam T. Sellen

    2009-01-01

    Full Text Available Las investigaciones que hemos desarrollado acerca de la historia de las colecciones yucatecas que forman parte del Museo Americano de Historia Natural de Nueva York nos permiten trazar un perfil general de los intereses intelectuales y de algunas de las primeras exploraciones emprendidas en la región. La información asociada a las piezas arqueológicas que actualmente se resguardan en dicho museo nos remite a los trabajos precursores llevados a cabo por reconocidos viajeros como Charnay, Stephens, Le Plongeon o Maler, y también de otros menos conocidos, como los coleccionistas locales. En conjunto, estos protoarqueólogos enfocaron sus esfuerzos a construir el conocimiento de la antigua civilización maya a través de sus evidencias materiales.Our research on the history of Yucatec collections that are now in the American Museum of Natural History in New York allows us to outline a general profile of the intellectual interests and some of the first explorations in the region. The information associated with the archaeological objects that are today held in this museum speak of the early archaeological work carried out by well known explorers such as Charnay, Stephens, Le Plongeon or Maler, and also by lesser-known players such as the local collectors. Together, these proto-archaeologists focused their efforts on constructing knowledge of the ancient Maya civilization through material evidence.

  6. Cdc42 regulates cofilin during the establishment of neuronal polarity

    DEFF Research Database (Denmark)

    Garvalov, Boyan K; Flynn, Kevin C; Neukirchen, Dorothee;

    2007-01-01

    The establishment of polarity is an essential process in early neuronal development. Although a number of molecules controlling neuronal polarity have been identified, genetic evidence about their physiological roles in this process is mostly lacking. We analyzed the consequences of loss of Cdc42......, a central regulator of polarity in multiple systems, on the polarization of mammalian neurons. Genetic ablation of Cdc42 in the brain led to multiple abnormalities, including striking defects in the formation of axonal tracts. Neurons from the Cdc42 null animals sprouted neurites but had a strongly...... suppressed ability to form axons both in vivo and in culture. This was accompanied by disrupted cytoskeletal organization, enlargement of the growth cones, and inhibition of filopodial dynamics. Axon formation in the knock-out neurons was rescued by manipulation of the actin cytoskeleton, indicating that the...

  7. Insights into Cdc13 Dependent Telomere Length Regulation

    Energy Technology Data Exchange (ETDEWEB)

    M Mason; E Skordalakes

    2011-12-31

    Cdc13 is a single stranded telomere binding protein that specifically localizes to the telomere ends of budding yeasts and is essential for cell viability. It caps the ends of chromosomes thus preventing chromosome end-to-end fusions and exonucleolytic degradation, events that could lead to genomic instability and senescence, the hallmark of aging. Cdc13 is also involved in telomere length regulation by recruiting or preventing access of telomerase to the telomeric overhang. Recruitment of telomerase to the telomeres for G-strand extension is required for continuous cell division, while preventing its access to the telomeres through capping the chromosome ends prevents mitotic events that could lead to cell immortality, the hall mark of carcinogenesis. Cdc13 and its putative homologues human CTC1 and POT1 are therefore key to many biological processes directly associated with life extension and cancer prevention and can be viewed as an ideal target for cancer and age related therapies.

  8. Grandes sistemas fluviais tropicais: uma visão geral

    Directory of Open Access Journals (Sweden)

    Rajiv Sinha

    2010-04-01

    Full Text Available Muitos dos grandes rios e dos leques aluviais existentes estão situados em áreas tropicais e constituem os maiores sistemas de estoque e transferência de sedimentos e nutrientes para o oceano. O presente trabalho visa a apresentação e análise geral dos grandes sistemas fluviais e leques aluviais (área da bacia entre 104 e 6 x 106 km de regiões tropicais com precipitação acima de 700 mm anuais. Os grandes sistemas fluviais tropicais foram aqui classificados de acordo com o arcabouço geológico-geomorfológio de suas bacias em: a Cinturões orogenênicos de montanha, b Platô/plataforma basáltico-sedimentar, c Áreas cratônicas, d Planícies em áreas de bacias sedimentares, e e Áreas mistas. De modo geral, os rios analisados apresentam um pico de descarga bastante definido e variável durante a estação chuvosa e um período de baixa vazão na estação seca. Os dados dos rios sul-americanos e asiáticos de áreas continentais e orogênicas agruparam-se quando lançados num diagrama do suprimento de sedimentos versus descarga média anual, e apresentaram valores mais elevados que aqueles asiáticos insulares. Já rios de áreas de plataforma ou cratônicas, tanto em área de savana como nos trópicos úmidos, caracterizaram-se por terem baixo transporte de sedimento. Especialmente nas grandes bacias, os rios apresentam toda a gama de formas de canal, inviabilizando a classificação tradicional de canal meandrante, reto e entrelaçado, sendo nesse caso mais sugestiva a aplicação da terminologia de sistema uni ou multi-canal ou de complexo anastomosado.É também discutido, brevemente, o atual estado do conhecimento dos sistemas fluviais tropicais e seu potencial na aplicação na interpretação de antigas seqüências e na modelagem de fácies sedimentar. Os principais impactos ambientais a que os grandes sistemas fluviais tropicais estão submetidos incluem as mudanças no uso agrosilpastoril das terras, mineração, barragens

  9. Sistemas de software distribuidos : Experiencias

    OpenAIRE

    Pesado, Patricia Mabel; Ramón, Hugo Dionisio; Thomas, Pablo Javier; Boracchia, Marcos; Bertone, Rodolfo Alfredo; Ibañez, Eduardo; Estrebou, César Armando; Marrero, Luciano; Delía, Lisandro; Galdámez, Nicolás; Cáseres, Germán

    2011-01-01

    Se presenta una línea de investigación y desarrollo, que tiene por objeto estudiar temas relacionados con aspectos de Ingeniería de Software orientados al desarrollo e implementación de proyectos concretos de Sistemas Distribuidos, en particular Sistemas Distribuidos con restricciones de Tiempo Real, Sistemas Web y Sistemas Sensibles al Contexto, considerando las extensiones necesarias en las metodologías y herramientas clásicas. En este sentido se trabaja en aspectos de la Ingeniería de Soft...

  10. Informe Signos Vitales de los CDC Obesidad infantil - (Childhood Obesity)

    Centers for Disease Control (CDC) Podcasts

    2013-08-06

    Este podcast se basa en el informe Signos Vitales de los CDC de agosto del 2013. La tasa de obesidad entre los niños en edad prescolar de bajos ingresos ha disminuido, pero todavía uno de cada seis niños hispanos es obeso. Este programa habla brevemente sobre lo que se puede hacer.  Created: 8/6/2013 by Centers for Disease Control and Prevention (CDC).   Date Released: 8/6/2013.

  11. Controlabilidad de Sistemas Bilineales en el Plano

    OpenAIRE

    Laura Guarachi, Leonardo Ramiro

    2012-01-01

    El objeto principal de este trabajo son los sistemas de ecuaciones diferenciales que dependen de un parámetro u, control del sistema, tales sistemas se llaman sistemas de ecuaciones diferenciales controlables, los cuales, en general, describen procesos dinámicos de fenómenos físicos. Estos procesos se describen mediante sistemas de ecuaciones diferenciales ordinarios (EDO), ecuaciones en diferencias o a través de ecuaciones derivadas parciales. Una clase de sistemas de mucha im...

  12. Sistema Brain Computer Interface

    OpenAIRE

    Martín Barraza, Juan Ignacio

    2015-01-01

    En este trabajo de final de grado se realizará una aplicación de un sistema Brain Computer Interface en el cual, a partir del dipositivo Mind Wave de la compañía Neurosky, se pretenderá controlar el prototipo de una mano humana. Esta será controlada a partir de las ondas cerebrales medidas por el sensor que el dispositivo dispone. A continuación, la información captada por nuestro medidor de señales de electroencefalográficas será enviada por radiofrecuencia a un stick USB que viene incorpora...

  13. Sistemas constructivos industrializados

    Directory of Open Access Journals (Sweden)

    del Águila, Alfonso

    1996-12-01

    Full Text Available The present article summarizes the main points discussed in three Innovator Buildings Systems sessions, which are about different subjects of Industrialized Building. The first part is about the topic of concrete panels, applied to building flats basically. It is given a historical summary and the general concepts, ending with "INDAGSA BUILDING SYSTEM", which is running several promotions with in Madrid. In the second part it is developed the subject of building with boxes, beginning with general points which are extended to the concept of box, its classification, its historical aspects and its applications. It is continues with a description of the "SET HOME SYSTEM" of boxes of concrete, which is dedicated to industrialization of social housing services and equipments. This part ends with the French system "TRANSLOKO" of big light boxes. Lately, it has made a building for laboratories in Madrid. The last part is dedicated to a new subject in Interior Building, which is the Dry Floor, being detailedly described the "PLADUR" point of view about this.

    El presente artículo resume lo tratado en tres jornadas de Sistemas Constructivos Innovadores, relativos a diversos temas de Construcción Industrializada. En la primera parte se trata el tema de los paneles de hormigón aplicados a la edificación, principalmente a viviendas. Se da una síntesis histórica y esquemática de conceptos generales, para terminar con una descripción del Sistema Constructivo INDAGSA que están realizando numerosas promociones en Madrid. La segunda parte, desarrolla el tema de la construcción con módulos tridimensionales, comenzando por unas consideraciones generales que abarcan el concepto de módulo tridimensional, su clasificación, aspectos históricos y campos de aplicación. Se sigue con una descripción del Sistema SET HOME, de módulos tridimensionales de hormigón, dedicado a la industrialización de servicios e instalaciones en vivienda social

  14. Sistemas de autoconsumo fotovoltaico

    OpenAIRE

    Pereira, Carlos Alberto da Conceição

    2015-01-01

    O presente relatório é desenvolvido no âmbito da unidade curricular de estágio do Mestrado em Engenharia Eletrotécnica com especialização em Controlo e Eletrónica Industrial e apresenta o meu trabalho na empresa na qual sou responsável pela parte de projeto, dimensionamento e supervisão de obra de sistemas fotovoltaicos para produção de energia. Os projetos apresentados neste relatório são da minha autoria. A empresa, Critical Kinetics apresenta-se no mercado como Consultores de energia...

  15. 77 FR 23733 - CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment

    Science.gov (United States)

    2012-04-20

    ... CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment In accordance with section 10(a)(2..., CDC, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, 1600 Clifton Road,...

  16. Continuous cell injury promotes hepatic tumorigenesis in cdc42-deficient mouse liver

    DEFF Research Database (Denmark)

    van Hengel, Jolanda; D'Hooge, Petra; Hooghe, Bart;

    2008-01-01

    BACKGROUND & AIMS: The Rho small guanosine triphosphatase Cdc42 is critical for diverse cellular functions, including regulation of actin organization, cell polarity, intracellular membrane trafficking, transcription, cell-cycle progression, and cell transformation. This implies that Cdc42 might be...

  17. A América Latina, uma justaposição de dois sistemas de proteção de direitos humanos?

    Directory of Open Access Journals (Sweden)

    Tenile Mascolo

    2015-08-01

    Full Text Available O MERCOSUL tem evoluído no estabelecimento de um sistema de promoção e proteção dos Direitos Humanos, principalmente graças ao desenvolvimento de uma estrutura institucional e normativa voltada à promoção e à proteção desses direitos. Tendo em vista que a maioria de seus Estados Partes são todos membros da Organização dos Estados Americanos, esses países se veem consequentemente sob os auspícios dos dois sistemas de proteção de Direitos Humanos, o que pode ser motivo para embates ou colaborações. Pergunta-se então de quem é a competência na apreciação de questões de violações referentes aos Direitos Humanos no seio do MERCOSUL: de um controle interno realizado pelos órgãos que compõem o sistema de resolução de conflitos do MERCOSUL ou de um controle externalizado pelos órgãos do sistema interamericano de proteção de Direitos Humanos?

  18. CDC Vital Signs: High Blood Pressure and Cholesterol

    Science.gov (United States)

    ... 1.36 MB] Read the MMWR Science Clips High Blood Pressure and Cholesterol Out of Control Recommend on Facebook ... by County http://apps.nccd.cdc.gov/GISCVH2/ High Blood Pressure and High Cholesterol Among US Adults SOURCES: National ...

  19. CDC 24/7: Saving Lives, Protecting People

    Centers for Disease Control (CDC) Podcasts

    2012-06-04

    24/7, CDC provides health information, responds to public health emergencies and natural disasters, and monitors disease.  Created: 6/4/2012 by Office of the Associate Director of Communciation (OADC).   Date Released: 6/4/2012.

  20. Child Passenger Safety (A Cup of Health with CDC)

    Centers for Disease Control (CDC) Podcasts

    2016-09-29

    Proper installation and use of car seats and booster seats for child passengers can save their lives. CDC recommends drivers ensure children are always buckled up. In this podcast, Bethany West discusses how to keep young passengers as safe as possible.  Created: 9/29/2016 by MMWR.   Date Released: 9/29/2016.

  1. CDC WONDER: a cooperative processing architecture for public health.

    Science.gov (United States)

    Friede, A; Rosen, D H; Reid, J A

    1994-01-01

    CDC WONDER is an information management architecture designed for public health. It provides access to information and communications without the user's needing to know the location of data or communication pathways and mechanisms. CDC WONDER users have access to extractions from some 40 databases; electronic mail (e-mail); and surveillance data processing. System components include the Remote Client, the Communications Server, the Queue Managers, and Data Servers and Process Servers. The Remote Client software resides in the user's machine; other components are at the Centers for Disease Control and Prevention (CDC). The Remote Client, the Communications Server, and the Applications Server provide access to the information and functions in the Data Servers and Process Servers. The system architecture is based on cooperative processing, and components are coupled via pure message passing, using several protocols. This architecture allows flexibility in the choice of hardware and software. One system limitation is that final results from some subsystems are obtained slowly. Although designed for public health, CDC WONDER could be useful for other disciplines that need flexible, integrated information exchange.

  2. Vaccines for Teens (A Minute of Health with CDC)

    Centers for Disease Control (CDC) Podcasts

    2016-08-25

    CDC recommends children aged 11 to 12 get three vaccines to protect from meningitis; cancers caused by human papillomavirus infections; and tetanus, diphtheria, and pertussis. This podcast discusses vaccination of adolescents.  Created: 8/25/2016 by MMWR.   Date Released: 8/25/2016.

  3. Sex Partner with No Zika Symptoms Transmits Virus: CDC

    Science.gov (United States)

    ... news/fullstory_160643.html Sex Partner With No Zika Symptoms Transmits Virus: CDC New report also highlights ties between Zika ... HealthDay News) -- U.S. health officials report that the Zika virus can be spread sexually even when a partner ...

  4. Dangerous Creatures - A Visit to the CDC Insectary

    Centers for Disease Control (CDC) Podcasts

    2012-11-07

    Tour CDC’s insectary with Sofi, a young host, and learn from CDC researchers about mosquitoes and insecticide resistance.  Created: 11/7/2012 by Center for Global Health (CGH).   Date Released: 12/20/2012.

  5. 77 FR 12845 - Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2012-03-02

    ... HUMAN SERVICES Centers for Disease Control and Prevention (CDC) Disease, Disability, and Injury Prevention and Control Special Emphasis Panel (SEP): Initial Review The meeting announced below concerns... Centers for Disease Control and Prevention/Agency for Toxic Substances and Disease Registry...

  6. Ciberreligiones: Aproximación al discurso religioso católico y afro-americano en Internet

    Directory of Open Access Journals (Sweden)

    Edgar A. Galavís Añez

    2003-01-01

    Full Text Available El presente artículo, basado en una investigación de carácter exploratorio cualitativo, refiere su corpus de análisis a dos ámbitos del hecho religioso: lo católico-cristiano y lo afro-americano,en relación a su despliegue icónico significante en la red multimediática Internet. El análisis estudiará la imagen como una función semiótica que se manifiesta en forma de discurso dentro de un contexto cultural telecomunicacional. La muestra se refiere a dos portales religiosos, seleccionados según criterios de densidad y calidad informativa: 1. Portal católico cristiano: EWTN/Global Catholic Network. 2.Portal Afroamericano: AxéAfonjá/Candomblé. La presencia de lo religioso en Internet conlleva al estudio de los elementos constitutivos y significantes de sus mensajes en relación al formato ciberespacial. Lo ciberreligioso sería entonces la unión de un nuevo espacio comunicacional con el discurso religioso. El análisis del corpus se apoyará en las retóricas audiovisuales de R. Barthes, U. Eco, en las indagaciones de G.Durandy M. Elia de sobre los arquetipos y los símbolos religiosos así como en la visión antropológica de Pollak Eltz sobre el Candomblé. La lectura se centrará en la estructura discursiva significante de los mitos y símbolos, sus aspectos iconográficos, hipertextuales y etnoescenológicos, en la dinámica diagramática de sus contenidos y en la conformación de sus redes de enlace con ellos mismos y con otras áreas de información paralela y complementaria.

  7. CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation

    NARCIS (Netherlands)

    Holzenspies, Jurriaan J.; Stoorvogel, Willem; Colenbrander, Ben; Roelen, Bernard A. J.; Gutknecht, Dagmar R.; van Haeften, Theo

    2009-01-01

    Background: Mammalian oocytes acquire competence to be fertilized during meiotic maturation. The protein kinase CDC2 plays a pivotal role in several key maturation events, in part through controlled changes in CDC2 localization. Although CDC2 is involved in initiation of maturation, a detailed analy

  8. Modeling the fission yeast cell cycle: Quantized cycle times in wee1 cdc25 mutant cells

    Science.gov (United States)

    Sveiczer, Akos; Csikasz-Nagy, Attila; Gyorffy, Bela; Tyson, John J.; Novak, Bela

    2000-07-01

    A detailed mathematical model for the fission yeast mitotic cycle is developed based on positive and negative feedback loops by which Cdc13/Cdc2 kinase activates and inactivates itself. Positive feedbacks are created by Cdc13/Cdc2-dependent phosphorylation of specific substrates: inactivating its negative regulators (Rum1, Ste9 and Wee1/Mik1) and activating its positive regulator (Cdc25). A slow negative feedback loop is turned on during mitosis by activation of Slp1/anaphase-promoting complex (APC), which indirectly re-activates the negative regulators, leading to a drop in Cdc13/Cdc2 activity and exit from mitosis. The model explains how fission yeast cells can exit mitosis in the absence of Ste9 (Cdc13 degradation) and Rum1 (an inhibitor of Cdc13/Cdc2). We also show that, if the positive feedback loops accelerating the G2/M transition (through Wee1 and Cdc25) are weak, then cells can reset back to G2 from early stages of mitosis by premature activation of the negative feedback loop. This resetting can happen more than once, resulting in a quantized distribution of cycle times, as observed experimentally in wee1- cdc25 mutant cells. Our quantitative description of these quantized cycles demonstrates the utility of mathematical modeling, because these cycles cannot be understood by intuitive arguments alone.

  9. 13 CFR 120.820 - CDC non-profit status and good standing.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false CDC non-profit status and good... CDC non-profit status and good standing. A CDC must be a non-profit corporation, except that for-profit CDCs certified by SBA prior to January 1, 1987 may retain their certifications. An SBIC may...

  10. Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1

    Directory of Open Access Journals (Sweden)

    Jose-Antonio Rodriguez-Rodriguez

    2016-05-01

    Full Text Available To complete mitosis, Saccharomyces cerevisiae needs to activate the mitotic phosphatase Cdc14. Two pathways contribute to Cdc14 regulation: FEAR (Cdc14 early anaphase release and MEN (mitotic exit network. Cdc5 polo-like kinase was found to be an important mitotic exit component. However, its specific role in mitotic exit regulation and its involvement in Cdc14 release remain unclear. Here, we provide insight into the mechanism by which Cdc5 contributes to the timely release of Cdc14. Our genetic and biochemical data indicate that Cdc5 acts in parallel with MEN during anaphase. This MEN-independent Cdc5 function requires active separase and activation by Cdk1-dependent phosphorylation. Cdk1 first phosphorylates Cdc5 to activate it in early anaphase, and then, in late anaphase, further phosphorylation of Cdc5 by Cdk1 is needed to promote its MEN-related functions.

  11. Sistema Presidencial Mexicano"

    Directory of Open Access Journals (Sweden)

    David Pantoja Morán

    2005-01-01

    Full Text Available En este artículo, el autor aborda el origen doctrinal de las dimensiones institucionales y constitucionales del sistema presidencial-constitucional mexicano para tratar de encontrar ahí los posibles orígenes o explicaciones del éxito o fracaso de este tipo de régimen político. Para ello, se recupera la relevancia y significado de la constitución de 1824 para la vida nacional —desde entonces hasta nuestros días— a fin de comprender muchos de los orígenes de los aciertos y de los errores en el diseño de nuestra constitución vigente así como de la forma presidencial de gobierno, a saber, el principio de legitimidad dual y el de la rigidez o separación rígida de los Poderes.

  12. Representações políticas do território latino-americano na Revista Seleções

    OpenAIRE

    Mary A. Junqueira

    2001-01-01

    Este artigo tem por objetivo analisar as representações sobre a América Latina construídas pela revista Seleções do Reader's Digest, entre 1942 e 1970, e mostra como essas representações estiveram informadas pela idéia de wilderness, profundamente enraizada no imaginário norte-americano.This article intends to analyse the representations about Latin America produced by Seleções do Reader's Digest magazine, from 1942 to 1970. It demonstrates that these representations were influenced by wilder...

  13. Representações políticas do território latino-americano na Revista Seleções

    Directory of Open Access Journals (Sweden)

    Mary A. Junqueira

    2001-01-01

    Full Text Available Este artigo tem por objetivo analisar as representações sobre a América Latina construídas pela revista Seleções do Reader's Digest, entre 1942 e 1970, e mostra como essas representações estiveram informadas pela idéia de wilderness, profundamente enraizada no imaginário norte-americano.This article intends to analyse the representations about Latin America produced by Seleções do Reader's Digest magazine, from 1942 to 1970. It demonstrates that these representations were influenced by wilderness imaginary, which is deeply rooted in North American culture.

  14. Comparação do uso de drogas entre universitários brasileiros, norte-americanos e jovens da população geral brasileira

    Directory of Open Access Journals (Sweden)

    Frederico Eckschmidt

    2013-09-01

    Full Text Available OBJETIVO: Comparar o uso de drogas entre os universitários e a população geral brasileira, assim como com os universitários norte-americanos, identificando possíveis diferenças de uso pela interferência da cultura. MÉTODOS: Para comparar o uso de drogas na vida entre as populações de interesse, foram utilizados os dados públicos de levantamentos estatísticos brasileiros realizados com universitários e a população geral, assim como levantamento estatístico norte-americano, por meio da comparação de intervalos de confiança (IC 95%. RESULTADOS: O uso de drogas na vida é mais frequente entre os estudantes norte-americanos, que relatam usar mais tabaco, tranquilizantes, maconha, ecstasy, alucinógenos, cocaína, crack e heroína que os universitários brasileiros. Em contrapartida, os universitários brasileiros relatam usar quase duas vezes mais inalantes do que os universitários norte-americanos. Esse padrão se repete ao se analisarem as diferenças intragênero. A isso se soma que os universitários brasileiros parecem envolver-se com mais frequência no uso de bebidas alcoólicas, maconha, tranquilizantes, inalantes, alucinógenos e anfetamínicos que seus pares da população geral brasileira. CONCLUSÕES: Conhecer as particularidades do uso de drogas entre os universitários é de suma importância para a detecção precoce desse consumo. Investimentos nesse segmento e diferenças de sexo devem ser contempladas nas políticas preventivas, assim como na elaboração de estratégias para a redução e o controle desse consumo, como programas de triagem e intervenção breve, uma vez que possuem a melhor relação custo-benefício.

  15. A Amazônia no imaginário norte-americano em tempo de guerra Amazonia in the U.S. wartime imaginary

    OpenAIRE

    Seth Garfield

    2009-01-01

    Este ensaio delineia a emergência da Amazônia no imaginário norte-americano no período da Segunda Guerra Mundial. Embora a importância geopolítica de diversificação dos mercados fornecedores de borracha e o desenvolvimento da borracha sintética tenham sido discutidos nos Estados Unidos antes do ataque a Pearl Harbor, a subsequente interdição japonesa aos mercados tradicionais do sudeste asiático levou burocratas e cientistas a se apressarem na busca de alternativas. Como em outras regiões da ...

  16. Diversidad genética de la población colombiana de ganado Cebú Brahman Americano Bos Indicus (Bovidae)

    OpenAIRE

    Novoa Bravo Miguel Adriano; Usaquén Martínez William

    2006-01-01

    La raza Cebú Brahman Americano se encuentra en Colombia alrededor de 100 años. Todo ese tiempo, esta raza ha estado bajo un proceso continuo de selección artificial dirigida, reproducción endogámica, efectos de deriva genética causados por eventos fundadores, migraciones de ejemplares entre las fincas del país y animales importados desde otros países. Estos hechos hacen a esta raza interesante y particular desde el punto de vista de la genética de poblaciones. El objetivo de este trabajo es e...

  17. Cdc18 transcription and proteolysis couple S phase to passage through mitosis.

    OpenAIRE

    Baum, B.; Nishitani, H; Yanow, S; Nurse, P

    1998-01-01

    In fission yeast, cdc18p plays a critical role in bringing about the onset of S phase. We show that cdc18p expression is subject to a complex sequence of cell cycle controls which ensure that cdc18p levels rise dramatically as cells exit mitosis, before the appearance of CDK activity in G1. We find that transcription of cdc18, together with the transcription of other cdc10p/res1p targets, is first initiated as cells enter mitosis and continues even in cells arrested in mitosis with highly con...

  18. Las virtudes del sistema binominal

    OpenAIRE

    John M Carey

    2006-01-01

    El sistema único de elecciones legislativas chileno, en el que cada distrito elige a dos representantes (M=2), ha sido objeto de controversia y de propuestas de reforma, ya que fue impuesto por el régimen autoritario saliente en 1990. La mayor parte de ellas aboga por la llamada mayor proporcionalidad. Este ensayo sostiene que, a pesar de sus orígenes no democráticos, el sistema M=2 ha funcionado relativamente bien respecto a los sistemas empleados en muchos otros países. En particular, M=2 e...

  19. Sistema de salud de Chile

    OpenAIRE

    Víctor Becerril-Montekio; Juan de Dios Reyes; Annick Manuel

    2011-01-01

    En este trabajo se describe el sistema de salud de Chile, incluyendo su estructura, financiamiento, beneficiarios y recursos físicos, materiales y humanos de los que dispone. Este sistema está compuesto por dos sectores, público y privado. El sector público está formado por todos los organismos que constituyen el Sistema Nacional de Servicios de Salud y cubre aproximadamente a 70% de la población, incluyendo a los pobres del campo y las ciudades, la clase media baja y los jubilados, así como ...

  20. A pós-graduação e a pesquisa no Serviço Social latino-americano: uma primeira aproximação Postgraduation and research in the Latin American Social Services: a preliminary approach

    Directory of Open Access Journals (Sweden)

    Carlos Montaño

    2011-12-01

    Full Text Available O artigo visa apresentar a sistematização dos dados sobre pós-graduação e pesquisa nos países hispano-americanos, a partir de documentos apresentados em dois eventos latino-americanos de pós-graduação. O objetivo é estimular a articulação, os convênios e o intercâmbio entre as unidades de ensino brasileiras e as dos demais países do subcontinente latino-americano.This article presents the systematization of data on postgraduation and research in Spanish American countries Such systematization was done from documents submitted in two Latin American postgraduation events. The goal is to stimulate the joint ventures, the agreements and the exchanges among Brazilian and other Latin American colleges.

  1. Quantitative analysis of membrane trafficking in regulation of Cdc42 polarity.

    Science.gov (United States)

    Watson, Leah J; Rossi, Guendalina; Brennwald, Patrick

    2014-12-01

    Vesicle delivery of Cdc42 has been proposed as an important mechanism for generating and maintaining Cdc42 polarity at the plasma membrane. This mechanism requires the density of Cdc42 on secretory vesicles to be equal to or higher than the plasma membrane polarity cap. Using a novel method to estimate Cdc42 levels on post-Golgi secretory vesicles in intact yeast cells, we: (1) determined that endocytosis plays an important role in Cdc42's association with secretory vesicles (2) found that a GFP-tag placed on the N-terminus of Cdc42 negatively impacts this vesicle association and (3) quantified the surface densities of Cdc42 on post-Golgi vesicles which revealed that the vesicle density of Cdc42 is three times more dilute than that at the polarity cap. This work suggests that the immediate consequence of secretory vesicle fusion with the plasma membrane polarity cap is to dilute the local Cdc42 surface density. This provides strong support for the model in which vesicle trafficking acts to negatively regulate Cdc42 polarity on the cell surface while also providing a means to recycle Cdc42 between the cell surface and internal membrane locations.

  2. Sistema polític espanyol: sistema electoral

    OpenAIRE

    Fernández Herrero, Myriam

    2012-01-01

    Materials docents programats mitjançant l'ajut del Servei de Política Lingüística de la Universitat de València. Explicacion de las funciones del sistema electoral en abstracto, de los elementos constitutivos y cómo lo definen. Aplicación al sistema electoral estatal, autonómico, local y europeo

  3. Two Cdc2 Kinase Genes with Distinct Functions in Vegetative and Infectious Hyphae in Fusarium graminearum.

    Directory of Open Access Journals (Sweden)

    Huiquan Liu

    2015-06-01

    Full Text Available Eukaryotic cell cycle involves a number of protein kinases important for the onset and progression through mitosis, most of which are well characterized in the budding and fission yeasts and conserved in other fungi. However, unlike the model yeast and filamentous fungi that have a single Cdc2 essential for cell cycle progression, the wheat scab fungus Fusarium graminearum contains two CDC2 orthologs. The cdc2A and cdc2B mutants had no obvious defects in growth rate and conidiation but deletion of both of them is lethal, indicating that these two CDC2 orthologs have redundant functions during vegetative growth and asexual reproduction. However, whereas the cdc2B mutant was normal, the cdc2A mutant was significantly reduced in virulence and rarely produced ascospores. Although deletion of CDC2A had no obvious effect on the formation of penetration branches or hyphopodia, the cdc2A mutant was limited in the differentiation and growth of infectious growth in wheat tissues. Therefore, CDC2A plays stage-specific roles in cell cycle regulation during infectious growth and sexual reproduction. Both CDC2A and CDC2B are constitutively expressed but only CDC2A was up-regulated during plant infection and ascosporogenesis. Localization of Cdc2A- GFP to the nucleus but not Cdc2B-GFP was observed in vegetative hyphae, ascospores, and infectious hyphae. Complementation assays with chimeric fusion constructs showed that both the N- and C-terminal regions of Cdc2A are important for its functions in pathogenesis and ascosporogenesis but only the N-terminal region is important for its subcellular localization. Among the Sordariomycetes, only three Fusarium species closely related to F. graminearum have two CDC2 genes. Furthermore, F. graminearum uniquely has two Aurora kinase genes and one additional putative cyclin gene, and its orthologs of CAK1 and other four essential mitotic kinases in the budding yeast are dispensable for viability. Overall, our data

  4. Plants of the American continent with antimalarial activity Plantas do continente Americano com atividade antimalárica

    Directory of Open Access Journals (Sweden)

    Ingrid R. Mariath

    2009-03-01

    Full Text Available Malaria is a human parasitic disease caused by protozoa species of the Plasmodium genus. This disease has affected populations of the tropical and subtropical regions. About 500 million new cases occur annually on the world and therefore it is considered an emerging disease of important public health problem. In this context, the natural products as vegetables species have their bioactive molecules as targets for pharmacological, toxicological and phytochemical studies towards the development of more effective medicines for the treatment of many diseases. So this work intends to aid the researchers in the study of natural products to the treatment of malaria. In this review, 476 plants of the American continent were related for the antimalarial activity and of these vegetables species 198 were active and 278 inactive for some type of Plasmodium when they were evaluated through of in vitro or in vivo bioassays models.Malária é uma doença parasitária humana causada por protozoários do gênero Plasmodium. Esta doença tem acometido populações que habitam regiões tropicais e subtropicais. Anualmente, cerca de 500 milhões de casos ocorrem no mundo, o que permite ser considerada uma doença emergente de importância para a saúde pública. Neste contexto, os produtos naturais, a exemplo das espécies vegetais, têm suas moléculas bioativas como alvo para estudos farmacológicos, toxicológicos e fitoquímicos destinados à síntese de medicamentos mais eficazes para o tratamento de inúmeras doenças. Portanto, este trabalho fornece subsídio às pesquisas com produtos naturais para o tratamento da malária. Nesta revisão, 476 espécies de plantas do continente Americano foram relatadas para a atividade antimalárica, sendo destas 198 ativas e 278 inativas para algum tipo de Plasmodium, quando avaliados através de modelos in vitro e in vivo.

  5. CDC Vital Signs-Communication Can Save Lives

    Centers for Disease Control (CDC) Podcasts

    2015-08-04

    This podcast is based on the August 2015 CDC Vital Signs report. Antibiotic-resistant germs cause at least 23,000 deaths each year. Learn how public health authorities and health care facilities can work together to save lives.  Created: 8/4/2015 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 8/4/2015.

  6. CDC Vital Signs-Hospital Actions Affect Breastfeeding

    Centers for Disease Control (CDC) Podcasts

    2015-10-06

    This podcast is based on the October 2015 CDC Vital Signs report. Hospitals can implement the Ten Steps to Successful Breastfeeding to be designated as "Baby-Friendly" and support more moms in a decision to breastfeed.  Created: 10/6/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/6/2015.

  7. CDC Vital Signs-Protect Patients from Antibiotic Resistance

    Centers for Disease Control (CDC) Podcasts

    2016-03-03

    This podcast is based on the March 2016 CDC Vital Signs report. Patients can get serious healthcare-associated infections, or HAIs, while receiving medical treatment in a healthcare facility. Learn how to prevent healthcare-associated infections.  Created: 3/3/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 3/3/2016.

  8. CDC Vital Signs-Safer Food Saves Lives

    Centers for Disease Control (CDC) Podcasts

    2015-11-03

    This podcast is based on the November 2015 CDC Vital Signs report. Contaminated food sent to several states can cause multistate outbreaks of foodborne illness and make a lot of people seriously ill. Learn what can be done to prevent and stop outbreaks.  Created: 11/3/2015 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 11/3/2015.

  9. Índices del Anuario de Estudios Americanos (tomos XLII-LI, 2, años 1985-1994

    Directory of Open Access Journals (Sweden)

    Sarabia Viejo, M.ª Justina

    1999-06-01

    Full Text Available Los índices que se incluyen a continuación tratan de aportar la información más útil a los investigadores americanistas. Para ello se ha establecido una división en índices alfabético, geográfico y por materias, además de situar al principio la relación de los artículos publicados en cada número del Anuario de Estudios Americanos durante esa década. Desde 1993, a partir del Tomo L (dividido en números 1 y 2 se ha unificado con la otra revista hasta entonces también publicada en la Escuela de Estudios Hispanoamericanos, del CSIC, con los nombres de Historiografía y Bibliografía Americanistas y Suplemento de Anuario de Estudios Americanos. Sección Historiografía y Bibliografía.

  10. El siglo perdido del Derecho Administrativo americano: más allá de un desfase histórico

    Directory of Open Access Journals (Sweden)

    Manuela Canal Silva

    2013-12-01

    Full Text Available Creando la Constitución Administrativa. Los Cien Años Perdidos del Derecho Administrativo americano, elegido por la American Bar Association –ABA– como el mejor trabajo de Derecho Administrativo publicado en el 2012, es considerado una de las más polémicas y exhaustivas exposiciones alrededor de la evolución del gobierno estadinense2. Acorde con las palabras de Robert L. Rabin (s.f., la obra es una contribución sustancial al entendimiento del gobierno administrativo en los Estados Unidos en sus orígenes y actualidad. Este artículo presenta las ideas más importantes del trabajo de Mashaw analizando la evolución de la Administración desde los primeros días de la República americana. Con este fin, explora la configuración del gobierno americano antes y después de la guerra civil. En conclusión, el artículo desarrolla la exposición de Mashaw y explora las implicaciones de su obra en las disciplinas histórica y jurídica americanas.

  11. Los indios americanos en las escenas líricas europeas: de los hermanos Purcell (1695 a Carl Heinrich Graun (1755

    Directory of Open Access Journals (Sweden)

    Núñez Ronchi, Ana

    2014-08-01

    Full Text Available In contrast to a supposedly critically-oriented Golden Age comedia, the first American- themed operas (the Purcells, Vivaldi, Rameau, Graun have been interpreted as uncritical expressions of the so-called Black Legend. Nevertheless, this judgment ought to be tempered: on the one hand, the Spanish comedia is not as censorious as has been asserted; on the other hand, although these operas do praise American Indians, they do not systematically belittle the Spaniards. Finally, the notion itself of “Black Legend” should be questioned.Se ha querido interpretar las primeras óperas de tema americano (Purcell, Vivaldi, Rameau y Graun como una expresión dramático-musical de la llamada Leyenda Negra, contrapuesta a una producción teatral áurea española que, inversamente, sí habría dejado espacio a la dimensión histórico-crítica. Ello nos parece injusto e inexacto: en primer lugar, la comedia áurea no escenifica la dimensión crítica que se ha pretendido atribuirle; en segundo lugar, el teatro lírico europeo, si bien exalta al indio americano, no devalúa sistemáticamente a los conquistadores españoles; finalmente, la noción misma de «Leyenda Negra» debe ser problematizada.

  12. Um exemplo de inversão do domínio ideológico norte-americano no Brasil

    Directory of Open Access Journals (Sweden)

    Carla Damião

    2014-07-01

    Full Text Available Propomos discutir a relação de domínio ideológico-cultural norte-americano sobre o Brasil, tendo em vista exemplos documentados. Trata-se da chamada “política de boa vizinhança” [Good Neighbor Policy], com início em 1933 até o final da Segunda Guerra, notadamente durante o governo Getúlio Vargas no Brasil e o governo Franklin Roosevelt nos Estados Unidos. A necessidade de propaganda política passava pela “sedução” cultural da potência estadunidense sobre seus vizinhos latino-americanos. Neste sentido, o momento político de tentativa de “adoção” ideológica do Brasil pelos Estados Unidos foi marcado pela “encomenda” de produtos culturais como a personagem do malandro Zé Carioca de Disney. O exemplo mais ambíguo que marca essa ambição de domínio ideológico é o filme encomendado a Orson Welles e jamais finalizado, intitulado: It’s All True [É tudo verdade]. O filme não concluído por seu famoso diretor, filmado em 1942, só se tornou conhecido em 1993 quando outros diretores americanos realizaram um documentário remontando partes do filme. O material presente nesse documentário foi também recuperado pela pesquisa realizada pelo cineasta brasileiro Rogério Sganzerla, da qual surgiu uma tetralogia composta pelos filmes: Nem tudo é verdade (1986, A liguagem de orson Welles (1990, Tudo é Brasil (1997 e O signo do caos (2003. O projeto de domínio cultural encomendado a Orson Welles terminou em fracasso, visto que este desviou sua atenção da glamourosa cidade do Rio de Janeiro e do Carnaval, para filmar a saga de jangadeiros que lutavam por direitos trabalhistas. A conotação política do filme determina seu fracasso no domínio ideológico de propaganda política do governo americano. Sganzerla elegeu o episódio para pensar e criticar o domínio ideológico norte-americano sempre presente na cultura brasileira, de uma maneira bastante ambígua. A ambiguidade permeia a atitude dos dois cineastas: o

  13. 13 CFR 120.837 - SBA decision on application for a new CDC or for an existing CDC to expand Area of Operations.

    Science.gov (United States)

    2010-01-01

    ... benchmarks, including pre-approval and annual review by SBA of any management or staff contracts, and the... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA decision on application for a new CDC or for an existing CDC to expand Area of Operations. 120.837 Section 120.837 Business...

  14. En portada SISTEMA RESPIRATORIO

    Directory of Open Access Journals (Sweden)

    Andrés J. Flores Alés,

    2005-10-01

    Full Text Available La sección “En portada” de este mes está dedicada a los agentes infecciosos y al diagnóstico de algunas de las enfermedades respiratorias más habituales en los animales de compañía, estacando la relevancia adquirida por el diagnóstico mediante la obtención de de imágenes en este campo. También se hace incapie en la actuación que debe llevar a cabo elínico ante una urgencia respiratoria La obtención de imágenes por radiología o endoscopia son dos de las técnicas diagnósticas que más ayudan a dilucidar el proceso respiratorio que sufre un paciente. Sobre el momento en el que se encuentra el diagnóstico por imagen de las patologías del sistema respiratorio en veterinaria nos habla a continuación Andrés J. Flores.

  15. Uma teoria da preponderância do Poder Executivo: o sistema de comissões no Legislativo brasileiro

    Directory of Open Access Journals (Sweden)

    Pereira Carlos

    2000-01-01

    Full Text Available O objetivo do artigo é analisar o sistema de comissões na Câmara dos Deputados à luz das teorias da escolha racional (distributiva, informacional e partidária desenvolvidas para explicar o Congresso norte-americano. Em que pese a enorme preponderância do Executivo sobre a agenda do Legislativo, este trabalho enfatiza que as comissões continuam funcionando ativamente e desempenham um papel importante para os parlamentares, os partidos e para o próprio Executivo. Inicialmente, tenta-se justificar a existência e funcionamento das comissões no Brasil com base na literatura distributiva. No entanto, como será demonstrado, esta explicação não pareceu ser suficientemente forte para justificar o atual sistema de comissões. Em seguida, através de uma adaptação da teoria informacional de Gilligan e Krehbiel (1997 para as especificidades das instituições do Congresso nacional, é testada uma hipótese usando os dados da legislatura 1995-1998. Os resultados da regressão multivariada (probit na escolha de procedimentos de urgência e não urgência (variável dependente fornecem evidências empíricas de que as comissões na Câmara dos Deputados têm de fato, pelo menos parcialmente, um papel informacional.

  16. Blastomicose do sistema nervoso

    Directory of Open Access Journals (Sweden)

    Horácio M. Canelas

    1951-09-01

    Full Text Available Os autores chamam a atenção sôbre os erros de diagnóstico nos casos de neuroblastomicose, seja nas formas meningoencefálicas, seja nas tumorais. São focalizadas a paracoccidioidose e a criptococose. Depois de caracterizarem clìnicamente a paracoccidioidose, acentuam a raridade das formas nervosas (1,2% dos casos dessa micose autopsiados no Departamento de Anatomia Patológica da Faculdade de Medicina da Universidade de São Paulo. Pela revisão da literatura foi verificado haver apenas 12 casos registrados. 0 caso de Casiello e Klass é o único em que o diagnóstico foi feito em vida; os demais, ou constituem achado de necropsia, ou foram inesperadamente revelados pelo exame histopatológico realizado após intervenções cirúrgicas. Os autores registram 2 casos de paracoccidioidose do sistema nervoso. No primeiro, tratava-se de meningomielorra-diculite crônica, sendo o parasito identificado no escarro; o segundo era portador de síndrome de compressão medular, cuja patogenia é discutida pelos autores, tendo sido a etiologia paracoccidióidica comprovada pelo exame da polpa e biópsia ganglionares. Considerações clínicas sôbre a criptococose precedem o relato de um caso de meningoencefalomielite subaguda, no qual fôra feito em vida o diagnóstico de paracoccidioidose, pelo exame micológico do liqüido cefalorraqueano; êste paciente, embora medicado com doses maciças de sulfa e tiossemicarbarsona, veio a falecer, tendo o exame anátomo-patológico revelado tratar-se, na realidade, de lesões nervosas produzidas pelo Crypíococcus neo-formans. Nos três casos era incisivo o caráter neurocirúrgico da sintomatologia. Entretanto, ante o diagnóstico de paracoccidioidose, foi instituído tratamento pelas sulfas, vacina específica e tiossemicarbarsona. Os resultados foram excelentes no caso 2, em que a terapêutica pôde ser instituída mais precocemente; melhoras também foram obtidas no caso 1. No caso 3, foram verificadas

  17. Sistemas de tiempo real y sistemas distribuidos de tiempo real

    OpenAIRE

    Pesado, Patricia; Ramón, Hugo Dionisio; Boracchia, Marcos; Pasini, Ariel C.; De Vito, María Cecilia; Osella Massa, Germán Leandro; Estrebou, César

    2006-01-01

    El proyecto de investigación y desarrollo en Sistemas de Software Distribuido, abarca aspectos que van desde los fundamentos del desarrollo (Técnicas de Ingeniería de Requerimientos, Metodologías de Gestión y Desarrollo de Proyectos, Técnicas de Planificación, Métricas, Normas de Calidad, Web-Systems) hasta la concepción de aplicaciones específicas orientadas a los procesos industriales (Sistemas de Planeamiento de Producción, Control Industrial en tiempo real), procesos de E-government (Voto...

  18. Sistema de monitorización de sistemas distribuidos

    OpenAIRE

    Vilas Virulegio, Alberto

    2014-01-01

    La computación distribuida ha estado presente desde hace unos cuantos años, pero es quizás en la actualidad cuando está contando con una mayor repercusión. En los últimos años el modelo de computación en la nube (Cloud computing) ha ganado mucha popularidad, prueba de ello es la cantidad de productos existentes. Todo sistema informático requiere ser controlado a través de sistemas de monitorización que permiten conocer el estado del mismo, de tal manera que pueda ser gestionado fácilmente....

  19. Enhanced membrane fluorescence of CDC-labelled paramecium subsequent to removal of surface components.

    Science.gov (United States)

    Wyroba, E; Bottiroli, G; Giordano, P

    1983-01-01

    Cytofluorimetric analysis of cycloheptaamylose-dansyl chloride (CDC) labelled Paramecium indicates that after mild trypsin removal of surface components the localization of CDC on the outer surface of living cells was not modified by the treatment. After such treatment the intensity of fluorescence emission was found about 3-fold higher in treated single cell than in the untreated one. These findings indicate that CDC labelling can be used to follow alteration occurred on the membrane of the living cell prior to labelling.

  20. Opposing Roles for Actin in Cdc42p PolarizationD⃞

    OpenAIRE

    Irazoqui, Javier E.; Howell, Audrey S.; Theesfeld, Chandra L.; Lew, Daniel J.

    2005-01-01

    In animal and fungal cells, the monomeric GTPase Cdc42p is a key regulator of cell polarity that itself exhibits a polarized distribution in asymmetric cells. Previous work showed that in budding yeast, Cdc42p polarization is unaffected by depolymerization of the actin cytoskeleton (Ayscough et al., J. Cell Biol. 137, 399–416, 1997). Surprisingly, we now report that unlike complete actin depolymerization, partial actin depolymerization leads to the dispersal of Cdc42p from the polarization si...

  1. Polo kinase Cdc5 is a central regulator of meiosis I

    OpenAIRE

    Attner, Michelle A.; Miller, Matthew P.; Ee, Ly-Sha; Elkin, Sheryl K; Amon, Angelika

    2013-01-01

    During meiosis, two consecutive rounds of chromosome segregation yield four haploid gametes from one diploid cell. The Polo kinase Cdc5 is required for meiotic progression, but how Cdc5 coordinates multiple cell-cycle events during meiosis I is not understood. Here we show that CDC5-dependent phosphorylation of Rec8, a subunit of the cohesin complex that links sister chromatids, is required for efficient cohesin removal from chromosome arms, which is a prerequisite for meiosis I chromosome se...

  2. Role of Cdc42-Cla4 interaction in the pheromone response of Saccharomyces cerevisiae.

    Science.gov (United States)

    Heinrich, Melanie; Köhler, Tim; Mösch, Hans-Ulrich

    2007-02-01

    In Saccharomyces cerevisiae, the highly conserved Rho-type GTPase Cdc42 is essential for cell division and controls cellular development during mating and invasive growth. The role of Cdc42 in mating has been controversial, but a number of previous studies suggest that the GTPase controls the mitogen-activated protein (MAP) kinase cascade by activating the p21-activated protein kinase (PAK) Ste20. To further explore the role of Cdc42 in pheromone-stimulated signaling, we isolated novel alleles of CDC42 that confer resistance to pheromone. We find that in CDC42(V36A) and CDC42(V36A, I182T) mutant strains, the inability to undergo pheromone-induced cell cycle arrest correlates with reduced phosphorylation of the mating MAP kinases Fus3 and Kss1 and with a decrease in mating efficiency. Furthermore, Cdc42(V36A) and Cdc42(V36A, I182T) proteins show reduced interaction with the PAK Cla4 but not with Ste20. We also show that deletion of CLA4 in a CDC42(V36A, I182T) mutant strain suppresses pheromone resistance and that overexpression of CLA4 interferes with pheromone-induced cell cycle arrest and MAP kinase phosphorylation in CDC42 wild-type strains. Our data indicate that Cla4 has the potential to act as a negative regulator of the mating pathway and that this function of the PAK might be under control of Cdc42. In conclusion, our study suggests that control of pheromone signaling by Cdc42 not only depends on Ste20 but also involves interaction of the GTPase with Cla4. PMID:17189484

  3. Diversidad genética de la población colombiana de ganado Cebú Brahman Americano Bos Indicus (Bovidae

    Directory of Open Access Journals (Sweden)

    Novoa Bravo Miguel Adriano

    2006-12-01

    Full Text Available La raza Cebú Brahman Americano se encuentra en Colombia alrededor de 100 años. Todo ese tiempo, esta raza ha estado bajo un proceso continuo de selección artificial dirigida, reproducción endogámica, efectos de deriva genética causados por eventos fundadores, migraciones de ejemplares entre las fincas del país y animales importados desde otros países. Estos hechos hacen a esta raza interesante y particular desde el punto de vista de la genética de poblaciones. El objetivo de este trabajo es estudiar la estructura y diversidad genética de la raza Cebú Brahman americano. Se utilizaron 162 animales registrados en la asociación colombiana de criadores de ganado cebú (ASOCEBU de 20 departamentos de Colombia. La genotipificación de los animales se llevó a cabo con el kit StockMarks® for cattle bovine genotyping de Applied Biosystems®, empleando 10 microsatélites dinucleótidos. Los resultados de los distintos análisis multivariados (Análisis de componentes principales y análisis de correspondencias múltiples, de inferencia bayesiana y distancias genéticas interindividuales, demuestran que no se presenta subestructura en la población, lo cual se explica por una alta tasa de migración de animales entre las diferentes fincas y regiones, que homogeniza las frecuencias en todo el país. Además, esta población posee un alto grado de heterocigocidad y diversidad alélica, comparado con otras razas, lo cual refleja su origen de mezcla multiracial. También se encontraron diferencias genéticas entre sexos, lo cual es causado por un proceso reproductivo diferencial, donde actúan diferentes criterios de selección entre sexos. Finalmente, al realizar un análisis de componentes principales para analizar las relaciones genéticas de Cebú Brahman americano colombiano con las razas cebuinas y taurinas, se determinó que esta raza se diferencia genéticamente de las demás razas cebuinas, debido a un aporte genético de razas taurinas

  4. Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase

    Institute of Scientific and Technical Information of China (English)

    YANG Hua; ZHENG Shu; MEIJER Laurent; LI Shi-min; LECLERC Sophie; YU Lin-lin; CHENG Jin-quan; ZHANG Su-zhan

    2005-01-01

    Objective: To screen and evaluate the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25phosphatase. Methods: The affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes are used as the cell cycle-specific targets for screening the antimitotic constituents. We tested 9 extracts isolated from the Chinese medicinal herbs and vegetables including the agents currently used in cancer treatment by measuring the inhibition of Cdc25A phosphatase and Cdc2 kinase activity. The antitumor activity of the extracts was also evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry.Results: Cdc25A inhibitory activity and antitumor activity are detected in the extracts isolated from three Chinese medicinal herbs Agrimonapilosa; Herba solani lyrati; Galla chinesis. Conclusion: We found three extracts isolated from Chinese medicinal herbs have potential inhibitory activity of Cdc25 phosphatase using a highly specific mechanism-based screen assay for antimitotic drug discovery.

  5. Rho GTPase protein Cdc42 is critical for postnatal cartilage development.

    Science.gov (United States)

    Nagahama, Ryo; Yamada, Atsushi; Tanaka, Junichi; Aizawa, Ryo; Suzuki, Dai; Kassai, Hidetoshi; Yamamoto, Matsuo; Mishima, Kenji; Aiba, Atsu; Maki, Koutaro; Kamijo, Ryutaro

    2016-02-19

    Cdc42, a small Rho GTPase family member, has been shown to regulate multiple cellular functions in vitro, including actin cytoskeletal reorganization, cell migration, proliferation, and gene expression. However, its tissue-specific roles in vivo remain largely unknown, especially in postnatal cartilage development, as cartilage-specific Cdc42 inactivated mice die within a few days after birth. In this study, we investigated the physiological functions of Cdc42 during cartilage development after birth using tamoxifen-induced cartilage-specific inactivated Cdc42 conditional knockout (Cdc42 (fl/fl); Col2-CreERT) mice, which were generated by crossing Cdc42 flox mice (Cdc42 (fl/fl)) with tamoxifen-induced type II collagen (Col2) Cre transgenic mice using a Cre/loxP system. The gross morphology of the Cdc42 cKO mice was shorter limbs and body, as well as reduced body weight as compared with the controls. In addition, severe defects were found in growth plate chondrocytes of the long bones, characterized by a shorter proliferating zone (PZ), wider hypertrophic zone (HZ), and loss of columnar organization of proliferating chondrocytes, resulting in delayed endochondral bone formation associated with abnormal bone growth. Our findings demonstrate the importance of Cdc42 for cartilage development during both embryonic and postnatal stages. PMID:26820532

  6. Amino acid residues in the CDC25 guanine nucleotide exchange factor critical for interaction with Ras.

    OpenAIRE

    Park, W.; Mosteller, R D; Broek, D.

    1994-01-01

    Previously we found that negatively charged residues at positions 62, 63, and 69 of H-Ras are involved in binding to the CDC25 guanine nucleotide exchange factor (GEF). Using site-directed mutagenesis, we have changed conserved, positively charged residues of CDC25GEF to glutamic acid. We find the nonfunctional CDC25R1374E mutant and the nonfunctional H-RasE63K mutant cooperate in suppression of the loss of CDC25 function in Saccharomyces cerevisiae. Also, peptides corresponding to residues 1...

  7. The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras.

    OpenAIRE

    Jones, S; Vignais, M L; Broach, J R

    1991-01-01

    The product of the CDC25 gene of Saccharomyces cerevisiae, in its capacity as an activator of the RAS/cyclic AMP pathway, is required for initiation of the cell cycle. In this report, we provide an identification of Cdc25p, the product of the CDC25 gene, and evidence that it promotes exchange of guanine nucleotides bound to Ras in vitro. Extracts of strains containing high levels of Cdc25p catalyze both removal of GDP from and the concurrent binding of GTP to Ras. This same activity is also o...

  8. Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p

    OpenAIRE

    Gladfelter, Amy S.; Bose, Indrani; Zyla, Trevin R.; Bardes, Elaine S.G.; Lew, Daniel J.

    2002-01-01

    At the beginning of the budding yeast cell cycle, the GTPase Cdc42p promotes the assembly of a ring of septins at the site of future bud emergence. Here, we present an analysis of cdc42 mutants that display specific defects in septin organization, which identifies an important role for GTP hydrolysis by Cdc42p in the assembly of the septin ring. The mutants show defects in basal or stimulated GTP hydrolysis, and the septin misorganization is suppressed by overexpression of a Cdc42p GTPase-act...

  9. Cdc13 N-Terminal Dimerization DNA Binding and Telomere Length Regulation

    Energy Technology Data Exchange (ETDEWEB)

    M Mitchell; J Smith; M Mason; S Harper; D Speicher; F Johnson; E Skordalakes

    2011-12-31

    The essential yeast protein Cdc13 facilitates chromosome end replication by recruiting telomerase to telomeres, and together with its interacting partners Stn1 and Ten1, it protects chromosome ends from nucleolytic attack, thus contributing to genome integrity. Although Cdc13 has been studied extensively, the precise role of its N-terminal domain (Cdc13N) in telomere length regulation remains unclear. Here we present a structural, biochemical, and functional characterization of Cdc13N. The structure reveals that this domain comprises an oligonucleotide/oligosaccharide binding (OB) fold and is involved in Cdc13 dimerization. Biochemical data show that Cdc13N weakly binds long, single-stranded, telomeric DNA in a fashion that is directly dependent on domain oligomerization. When introduced into full-length Cdc13 in vivo, point mutations that prevented Cdc13N dimerization or DNA binding caused telomere shortening or lengthening, respectively. The multiple DNA binding domains and dimeric nature of Cdc13 offer unique insights into how it coordinates the recruitment and regulation of telomerase access to the telomeres.

  10. CDC Vital Signs-E-cigarette Ads and Youth

    Centers for Disease Control (CDC) Podcasts

    2016-01-05

    This podcast is based on the January 2016 CDC Vital Signs report. Most electronic cigarettes, or e-cigarettes, contain nicotine, which is highly addictive and may harm brain development. More than 18 million middle and high school students were exposed to e-cigarette ads. Exposure to these ads may be contributing to an increase in e-cigarette use among youth. Learn what can be done to keep our youth safe and healthy.  Created: 1/5/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 1/5/2016.

  11. CDC Vital Signs-Daily Pill Can Prevent HIV

    Centers for Disease Control (CDC) Podcasts

    2015-11-24

    This podcast is based on the November 24, 2015 CDC Vital Signs report. Preexposure prophylaxis, or PrEP, is a daily medicine that can be used to prevent getting HIV. PrEP is for people who don’t have HIV but who are at very high risk for getting it from sex or injection drug use. Unfortunately, many people who can benefit from PrEP aren’t taking it.  Created: 11/24/2015 by National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP).   Date Released: 11/24/2015.

  12. Problemas actuales del sistema penitenciario

    OpenAIRE

    González Martín, Jennifer

    2014-01-01

    He pretendido con este proyecto analizar los cambios que actualmente está sufriendo nuestro sistema penitenciario desde una óptica meramente jurídica. A tal fin, este trabajo se centra en analizar, por una parte, el proyecto de reforma del Código Penal centrándose en los aspectos que afectan al sistema penitenciario (en el caso de que finalmente sea aprobado por las Cortes Generales), y, por otra parte, se estudia la conocida como "Doctrina Parot" analizando en qué consiste, cómo se implantó ...

  13. Sistema de coberta transitable ventilada

    OpenAIRE

    Pagés Serra, Jorge

    2013-01-01

    Investigació referent a l'aplicació pràctica d'un sistema constructiu per a realitzar una coberta ventilada transitable amb paviment continuu En el nostre clima la màxima insolació es produeix a la coberta. Per altre banda les cobertes a la catalana o cobertes ventilades, tant comuns a l'eixample i d'altres zones de barcelona han pràcticament desaparegut. Aquesta investigació preten desenvolupar un sistema constructiu que permeti la realització de cobertes ventilades amb paviments continus...

  14. Sistemas de software distribuido : Aplicaciones

    OpenAIRE

    De Giusti, Armando Eduardo; Pesado, Patricia; Bertone, Rodolfo Alfredo; Boracchia, Marcos; Thomas, Pablo Javier; Madoz, María Cristina

    2002-01-01

    Realizar investigación y desarrollo en temas relacionados con Sistemas de Software Distribuido, particularmente los relacionados con los aspectos de Ingeniería de Software que se orientan al desarrollo e implementación de proyectos de sistemas distribuidos, incluyendo especialmente los temas de métricas y calidad de software. Estas tareas abarcan aspectos propios de la arquitectura, comunicaciones, estructuración de bases de datos, así como especificación, validación y desarrollo de aplica...

  15. Un sistema de restricciones anotado

    OpenAIRE

    RAMÍREZ OVALLE, CARLOS ERNESTO

    2010-01-01

    En la programación concurrente con restricciones, el concepto básico es el de sistema de restricciones. Aquí presentamos una innovación sobre los sistemas de restricciones al introducir una lógica anotada como componente sintáctico y semántico junto con un motor de inferencia lógica en hiper-resolución anotada. Esto permite incrementar la capacidad expresiva de un modelo basado en satisfacción de restricciones pues es ahora posible incluir información adicional a las restricciones clásicas de...

  16. El sistema reticulo-endotelial

    OpenAIRE

    Cuartas L., Carlos J.

    2011-01-01

    Definición.-Este nombre fue dado por Aschoff a una categoríacelular, cuyos elementos se encuentran profusamente distribuídos en la trama de sostén de diferentes órganos y tejidos; esos elementos gozan de características funcionales que justifican su individualización en un sistema celular especial. Muchos son los trabajos de investigación que han puesto de manifiesto las propiedades de este sistema celular, a pesar de su conocimiento relativamente reciente. Gracias a ellos, sabemos que esos e...

  17. Sistema de incendios inalámbrico

    OpenAIRE

    Menéndez Muñiz, Sergio

    2012-01-01

    Sistema de detección de incendios inalámbrico basado en dispositivos "motes" para la monitorización del estado del sistema, detección y reconocimiento de alarmas. Sistema de detecció d'incendis sense fils basat en dispositius "motes" per a la monitorització de l'estat del sistema, detecció i reconeixement d'alarmes.

  18. Trained to interact: echoes from the Workshop Sul-Americano de Mediação em Museus e Centros de Ciência (Italian original version

    Directory of Open Access Journals (Sweden)

    Luisa Massarani

    2008-12-01

    Full Text Available The initiatives focusing the professional development of explainers are multiplying around the world, building an informal network of researchers, museums managers and directors, explainers, and regional/continental networks, as THE group, the Thematic Human Interface and Explainers group of Ecsite.The Workshop Sul-Americano de Mediação em Museus e Centros de Ciência e Escola de Mediação em Museus e Centros de Ciência, which took place in Rio de Janeiro in September 2008, was a further important step along this path. We believe it is worthwhile to offer to Jcom readers some of the workshop contributions concerning the training of explainers, to which we added an overview of the general problem presented by Lynn Uyen Tran (Lawrence Hall of Science at the University of California, Berkeley.

  19. Modelo de avaliação de uma opção de venda tipo Americano: solução analítica.

    OpenAIRE

    Viegas, Cristina; Pereira, José Azevedo

    2005-01-01

    Este estudo desenvolve uma solução analítica para o valor de uma opção de venda de tipo Americano e para o respectivo preço crítico. Para obter uma solução fechada da equação diferencial proposta por Black e Scholes (1973), utiliza-se um procedimento usualmente conhecido como “método das linhas” - considera-se uma formulação na qual o tempo é discreto, em vez de contínuo. Deste modo, a equação diferencial parcial é transformada numa equação diferencial ordinária não homogénea, a derivad...

  20. Vitalism, Idéologie and Physiology in Buenos Aires. The Debate between Cosme Argerich and Crisóstomo Lafinur in El Americano, 1819

    Directory of Open Access Journals (Sweden)

    Mariano Di Pasquale

    2016-03-01

    Full Text Available Objective: this article aims to analyze the intellectual dispute between the doctor Cosme Mariano Argerich and Juan Crisóstomo Lafinur, philosophy professor. The debate located in the Buenos Aires area and recorded in the press in the journal El Americano originated by the introduction of new teaching linked to the sensualism and the French physiology at the school Unión del Sud in 1819. Content: the article wants to demonstrate the existence of a process of appropriation of knowledge that made these local actors according their interests and positions. Conclusions: the purpose of Argerich and Lafinur was to build consistent arguments in order to convince an incipient public sphere about sensualism and physiology respectively. In their quest to win the dispute, both actors perfected their demonstrations so you can see a different discursive level for the original notions of European authors.

  1. 光滑念珠菌 Cdc42基因生物信息分析%Bioinformatics Analysis of Cdc42 Gene from Candida Glabrata

    Institute of Scientific and Technical Information of China (English)

    赵静; 黄怀球; 袁立燕; 钟毅; 张静; 张晓辉

    2013-01-01

    目的:分析和预测光滑念珠菌Cdc42基因及其编码蛋白的结构和特性。方法:利用NCBI、Ex-PASy和CBS网站中的各种信息分析工具,并结合Vector NTI suite 8.0生物信息学分析软件包,分析预测光滑念珠菌Cdc42基因并预测该基因编码蛋白结构的特征和功能。结果:Cdc42基因全长为576 bp,编码区具有191个氨基酸,在GenBank同源序列中,其与酵母 Cdc42氨基酸序列一致性达到99%,且有Cdc42保守域。 Cdc42蛋白相对分子量预测为21420.83,理论等电点为6.31。预测Cdc42编码蛋白ɑ螺旋(H)、β折叠(E)、无规则卷(L)的比例分别是29.84%、28.70%、41.88%,1个GTP/ATP结合位点。 Cdc42蛋白为疏水蛋白,无跨膜区,无信号肽。结论:成功预测Cdc42基因及编码蛋白生化及结构特征,为下一步对其进行克隆和表达奠定基础。%Objective:To analyze and predict the structure and properties about encoding pro-tein of cell division cycle 42(Cdc42) from Candida glabrata by bioinformatics.Methods:A full-length cDNA sequence encoding Cdc 42 from Candida glabrata was identified by using tools of bioinformatics at webs sites of NCBI , ExPASy, CBS and software Vector NTI suite 8.0.The char-acteristics of the protein were predicted by employing bioinformatics software package supplied by the website of ExPaSy .Results:The full length of Cdc42 is 576 bp, and its ORF encodes 191 ami-no acid.The relationship of phylogenesis between Candida glabrata and other fungus is close .The prediction shows that Cdc 42 had a Cdc42 conserved domain , the molecular weight and theoretical pI of Cg.Cdc42 was 21 420.83 and 6.31 respectively, and the coding protein contains 29.84%ɑ-helix, 28.70%extended strand,41.88% random coil,and one GTP/ATP motif.Cdc42 enco-ding protein is hydrophobic , extra-membrane protein , without signal peptide .Conclusion:The structure and characteristics of the gene and protein of Cg .Cdc42 was

  2. El sistema tributario japonés

    OpenAIRE

    Yoshiyasu Okada

    2002-01-01

    Este documento primeramente trata las tendencias recientes y el estado actual del sistema tributario japonés como antecedentes. Luego resume los impuestos principales y describe el actual sistema. Finalmente trata los incentivos tributarios y los aspectos internacionales del sistema japonés.

  3. CDC Signos Vitales: Enfermedad del legionario (CDC Vital Signs–Legionnaires’ Disease)

    Centers for Disease Control (CDC) Podcasts

    2016-06-07

    Este podcast se basa en la edición de junio del 2016 del informe Signos Vitales de los CDC. Las personas pueden contraer la enfermedad del legionario, un tipo grave de infección pulmonar, al inhalar pequeñas gotitas de agua contaminada con bacterias Legionella. Obtenga más información sobre lo que se puede hacer para ayudar a prevenir brotes de enfermedad del legionario y mantener seguras a las personas.  Created: 6/7/2016 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 6/7/2016.

  4. Multisite phosphorylation of the guanine nucleotide exchange factor Cdc24 during yeast cell polarization.

    Directory of Open Access Journals (Sweden)

    Stephanie C Wai

    Full Text Available BACKGROUND: Cell polarization is essential for processes such as cell migration and asymmetric cell division. A common regulator of cell polarization in most eukaryotic cells is the conserved Rho GTPase, Cdc42. In budding yeast, Cdc42 is activated by a single guanine nucleotide exchange factor, Cdc24. The mechanistic details of Cdc24 activation at the onset of yeast cell polarization are unclear. Previous studies have suggested an important role for phosphorylation of Cdc24, which may regulate activity or function of the protein, representing a key step in the symmetry breaking process. METHODOLOGY/PRINCIPAL FINDINGS: Here, we directly ask whether multisite phosphorylation of Cdc24 plays a role in its regulation. We identify through mass spectrometry analysis over thirty putative in vivo phosphorylation sites. We first focus on sites matching consensus sequences for cyclin-dependent and p21-activated kinases, two kinase families that have been previously shown to phosphorylate Cdc24. Through site-directed mutagenesis, yeast genetics, and light and fluorescence microscopy, we show that nonphosphorylatable mutations of these consensus sites do not lead to any detectable consequences on growth rate, morphology, kinetics of polarization, or localization of the mutant protein. We do, however, observe a change in the mobility shift of mutant Cdc24 proteins on SDS-PAGE, suggesting that we have indeed perturbed its phosphorylation. Finally, we show that mutation of all identified phosphorylation sites does not cause observable defects in growth rate or morphology. CONCLUSIONS/SIGNIFICANCE: We conclude that lack of phosphorylation on Cdc24 has no overt functional consequences in budding yeast. Yeast cell polarization may be more tightly regulated by inactivation of Cdc42 by GTPase activating proteins or by alternative methods of Cdc24 regulation, such as conformational changes or oligomerization.

  5. Thoughts on Seeing "El Sistema"

    Science.gov (United States)

    Booth, Eric

    2009-01-01

    The FESNOJIV (the Spanish acronym for the State Foundation for the National System of Youth and Children's Orchestras), more commonly known as "El Sistema," teaches 300,000 of Venezuela's poorest children in the nationwide music learning program. Many begin attending the "nucleo" as early as age two or three, and the vast majority continue well…

  6. Knowledge Exchange with Sistema Scotland

    Science.gov (United States)

    Allan, Julie; Moran, Nikki; Duffy, Celia; Loening, Gica

    2010-01-01

    This paper reports on a knowledge exchange project, funded by the Scottish Funding Council and undertaken by a group of researchers from three higher education institutions in Scotland and the project partner, Sistema Scotland. This newly established charity is attempting to implement a major programme of social change, developed in Venezuela,…

  7. Exposiciones rábicas en Colombia: evaluación del sistema de vigilancia desde los actores/ Rabies exposures in Colombia: an evaluation by the actors of the surveillance system

    OpenAIRE

    Claudia P. Roncancio-Melgarejo; Daniel A. Buitrago-Medina; Isabel C. Posada-Zapata; Hugo Grisales-Romero

    2015-01-01

    Resumen Objetivo: identificar la percepción que tienen los actores frente a los atributos del sistema de vigilancia de las exposiciones rábicas para generar las recomendaciones que se requieran. Metodología: se utilizó la teoría fundada hasta análisis de categorías interpretativas; once entrevistas semiestructuradas enmarcadas en los atributos considerados por los Centros de Control y Prevención de Enfermedades (cdc) para la evaluación de sistemas de vigilancia se realizaron a personas con...

  8. Metodologia de sistemas de data warehouse

    OpenAIRE

    Sá, Jorge Vaz de Oliveira e

    2010-01-01

    Tese de doutoramento em Tecnologias e Sistemas de Informação (área de conhecimento em Engenharia e Gestão de Sistemas de Informação) Os sistemas de Data Warehouse possibilitam que as organizações tenham acesso a informação de gestão que é essencial para a vida da organização. No entanto a implementação destes sistemas nas organizações sofre de uma elevada taxa de insucesso. Várias iniciativas de implementação de Sistemas de Data Warehouse arrancaram com grandes expectativas, mas r...

  9. Las virtudes del sistema binominal

    Directory of Open Access Journals (Sweden)

    John M Carey

    2006-01-01

    Full Text Available El sistema único de elecciones legislativas chileno, en el que cada distrito elige a dos representantes (M=2, ha sido objeto de controversia y de propuestas de reforma, ya que fue impuesto por el régimen autoritario saliente en 1990. La mayor parte de ellas aboga por la llamada mayor proporcionalidad. Este ensayo sostiene que, a pesar de sus orígenes no democráticos, el sistema M=2 ha funcionado relativamente bien respecto a los sistemas empleados en muchos otros países. En particular, M=2 en Chile entrega una mezcla adecuada de representatividad, gobernabilidad y responsabilidad individual del legislador. El ensayo considera varias alternativas para la reforma y perfila algunos problemas potenciales que cada una implicaría. Si se reforma el sistema chileno, entonces el autor recomienda que se haga por pasos incrementales, no fundamentales -un aumento moderado en la magnitud de cada distrito-, pero manteniendo las listas abiertas y no haciendo el salto a un sistema mixto de miembros (SMMChile's unique system of legislative elections, in which each district elects two representatives (M=2, has been subject to controversy and a target for reform proposals since it was imposed on the country by the outgoing authoritarian regime in 1990. Most reform advocates call for greater proportionality. This essay argues that, desipte its undemocratic origins, the M=2 system has performed quite well relative to systems used in many other countries. In particular, M=2 in Chile delivers a reasonable mix of representativeness, governability, and individual legislator accountability. The essay goes on to consider various alternatives for reform and outlines some potential problems each would entail. If the Chilean system is to be reformed, the essay recommends incrementalism - a moderate increase in district magnitude, perhaps, but maintaining open lists, and no change to a mixed-member system (MMS

  10. Introducción al estudio de los sistemas económicos

    OpenAIRE

    García Nossa, Antonio

    2010-01-01

    Interdependencia de los sistemas; Coexistencia de los sistemas; Sucesión y coexistencia; Sistemas históricos y sistemas geopolíticos; Factores cuantitativos y cualitativos de los sistemas; Principios generales de los sistemas.

  11. The human homolog of S. cerevisiae CDC27, CDC27 Hs, is encoded by a highly conserved intronless gene present in multiple copies in the human genome

    Energy Technology Data Exchange (ETDEWEB)

    Devor, E.J.; Dill-Devor, R.M. [Univ. of Iowa College of Medicine, Iowa City (United States)

    1994-09-01

    We have obtained a number of unique sequences via PCR amplification of human genomic DNA using degenerate primers under low stringency (42{degrees}C). One of these, an 853 bp product, has been identified as a partial genomic sequence of the human homolog of the S. cerevisiae CDC27 gene, CDC27Hs (GenBank No. U00001). This gene, reported by Turgendreich et al. is also designated EST00556 from Adams et al. We have undertaken a more detailed examination of our sequence, MCP34N, and have found that: 1. the genomic sequence is nearly identical to CDC27Hs over its entire 853 bp length; 2. an MCP34N-specific PCR assay of several non-human primate species reveals amplification products in chimpanzee and gorilla genomes having greater than 90% sequence identity with CDC27Hs; and 3. an MCP34N-specific PCR assay of the BIOS hybrid cell line panel gives a discordancy pattern suggesting multiple loci. Based upon these data, we present the following initial characterization: 1. the complete MCP34N sequence identity with CDC27Hs indicates that the latter is encoded by an intronless gene; 2. CDC27Hs is highly conserved among higher primates; and 3. CDC27Hs is present in multiple copies in the human genome. These characteristics, taken together with those initially reported for CDC27Hs, suggest that this is an old gene that carries out an important but, as yet, unknown function in the human brain.

  12. Distinct roles of Cdc42 in thymopoiesis and effector and memory T cell differentiation.

    Directory of Open Access Journals (Sweden)

    Fukun Guo

    Full Text Available Cdc42 of the Rho GTPase family has been implicated in cell actin organization, proliferation, survival, and migration but its physiological role is likely cell-type specific. By a T cell-specific deletion of Cdc42 in mouse, we have recently shown that Cdc42 maintains naïve T cell homeostasis through promoting cell survival and suppressing T cell activation. Here we have further investigated the involvement of Cdc42 in multiple stages of T cell differentiation. We found that in Cdc42(-/- thymus, positive selection of CD4(+CD8(+ double-positive thymocytes was defective, CD4(+ and CD8(+ single-positive thymocytes were impaired in migration and showed an increase in cell apoptosis triggered by anti-CD3/-CD28 antibodies, and thymocytes were hyporesponsive to anti-CD3/-CD28-induced cell proliferation and hyperresponsive to anti-CD3/-CD28-stimulated MAP kinase activation. At the periphery, Cdc42-deficient naive T cells displayed an impaired actin polymerization and TCR clustering during the formation of mature immunological synapse, and showed an enhanced differentiation to Th1 and CD8(+ effector and memory cells in vitro and in vivo. Finally, Cdc42(-/- mice exhibited exacerbated liver damage in an induced autoimmune disease model. Collectively, these data establish that Cdc42 is critically involved in thymopoiesis and plays a restrictive role in effector and memory T cell differentiation and autoimmunity.

  13. 76 FR 66721 - CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment

    Science.gov (United States)

    2011-10-27

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention CDC/HRSA Advisory Committee on HIV and STD...-Cseh, CDC, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, 1600 Clifton Road,...

  14. Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy.

    Directory of Open Access Journals (Sweden)

    Ronan Broderick

    Full Text Available Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our data show that eGFP-Cdc45 mobility is faster in G1/S transition compared to S phase suggesting that Cdc45 is part of larger protein complex formed in S phase. Furthermore, the size of complexes containing Cdc45 was estimated in asynchronous, G1/S and S phase-synchronized cells using gel filtration chromatography; these findings complemented the in vivo FCS data. Analysis of the mobility of eGFP-Cdc45 and the size of complexes containing Cdc45 and eGFP-Cdc45 after UVC-mediated DNA damage revealed no significant changes in diffusion rates and complex sizes using FCS and gel filtration chromatography analyses. This suggests that after UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage.

  15. 77 FR 58847 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2012-09-24

    ... HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) In accordance with section 10(a)(2) of the Federal... enable CDC to fulfill its mission of protecting health through health promotion, prevention,...

  16. CDC's Response to the 2014-2016 Ebola Epidemic - Guinea, Liberia, and Sierra Leone.

    Science.gov (United States)

    Dahl, Benjamin A; Kinzer, Michael H; Raghunathan, Pratima L; Christie, Athalia; De Cock, Kevin M; Mahoney, Frank; Bennett, Sarah D; Hersey, Sara; Morgan, Oliver W

    2016-01-01

    CDC's response to the 2014-2016 Ebola virus disease (Ebola) epidemic in West Africa was the largest in the agency's history and occurred in a geographic area where CDC had little operational presence. Approximately 1,450 CDC responders were deployed to Guinea, Liberia, and Sierra Leone since the start of the response in July 2014 to the end of the response at the end of March 2016, including 455 persons with repeat deployments. The responses undertaken in each country shared some similarities but also required unique strategies specific to individual country needs. The size and duration of the response challenged CDC in several ways, particularly with regard to staffing. The lessons learned from this epidemic will strengthen CDC's ability to respond to future public health emergencies. These lessons include the importance of ongoing partnerships with ministries of health in resource-limited countries and regions, a cadre of trained CDC staff who are ready to be deployed, and development of ongoing working relationships with U.S. government agencies and other multilateral and nongovernment organizations that deploy for international public health emergencies. CDC's establishment of a Global Rapid Response Team in June 2015 is anticipated to meet some of these challenges. The activities summarized in this report would not have been possible without collaboration with many U.S. and international partners (http://www.cdc.gov/vhf/ebola/outbreaks/2014-west-africa/partners.html). PMID:27388930

  17. Testing public health ethics: why the CDC's HIV screening recommendations may violate the least infringement principle.

    Science.gov (United States)

    Pierce, Matthew W; Maman, Suzanne; Groves, Allison K; King, Elizabeth J; Wyckoff, Sarah C

    2011-01-01

    The CDC's HIV screening recommendations for health care settings advocate abandoning two important autonomy protections: (1) pretest counseling and (2) the requirement that providers obtain affirmative agreement from patients prior to testing. The recommendations may violate the least infringement principle because there is insufficient evidence to conclude that abandoning pretest counseling or affirmative agreement requirements will further the CDC's stated public health goals.

  18. "Working Together, Unlimited Things Can Happen": CDC, Tribes, Colleges Strive to Improve Native Health

    Science.gov (United States)

    Selden, Ron

    2004-01-01

    The article focuses on the overall health of American Indians. Native people living on reservations and in urban areas face a broad array of health problems. Centers for Disease Control and Prevention (CDC) in Atlanta is committed to improving the health of Native Americans. CDC is one of the agencies within the U.S. Department of Health and Human…

  19. Do the benefits of male circumcision outweigh the risks? A critique of the proposed CDC guidelines

    Directory of Open Access Journals (Sweden)

    Brian D. Earp

    2015-03-01

    Full Text Available The Centers for Disease Control and Prevention (CDC have announced a set of provisional guidelines concerning male circumcision, in which they suggest that the benefits of the surgery outweigh the risks. In this perspective article, I highlight a few of the key scientific and ethical issues worth considering in interpreting the new CDC recommendations.

  20. Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein.

    Directory of Open Access Journals (Sweden)

    Lia Rosso

    2008-06-01

    Full Text Available Gene duplication was prevalent during hominoid evolution, yet little is known about the functional fate of new ape gene copies. We characterized the CDC14B cell cycle gene and the functional evolution of its hominoid-specific daughter gene, CDC14Bretro. We found that CDC14B encodes four different splice isoforms that show different subcellular localizations (nucleus or microtubule-associated and functional properties. A microtubular CDC14B variant spawned CDC14Bretro through retroposition in the hominoid ancestor 18-25 million years ago (Mya. CDC14Bretro evolved brain-/testis-specific expression after the duplication event and experienced a short period of intense positive selection in the African ape ancestor 7-12 Mya. Using resurrected ancestral protein variants, we demonstrate that by virtue of amino acid substitutions in distinct protein regions during this time, the subcellular localization of CDC14Bretro progressively shifted from the association with microtubules (stabilizing them to an association with the endoplasmic reticulum. CDC14Bretro evolution represents a paradigm example of rapid, selectively driven subcellular relocalization, thus revealing a novel mode for the emergence of new gene function.

  1. Characterization of centralizers on CDC-algebras%CDC-代数上中心化子的刻画

    Institute of Scientific and Technical Information of China (English)

    马飞; 张建华; 尹琳娟

    2015-01-01

    设 A lg L是Hilbert空间 H 上的一个CDC-代数,φ:A lg L ® A lg L是一可加映射。证明了如果存在正整数m'n1,满足对于任意的a Î A lg L'有φ(am+n+1)=amφ(a)an ,那么存在 A lg L的中心中的元素λÎ Z(A lg L),使得对于任意的a Î A lg L'有φ(a)=λa。%Let A lg L be a CDC-algebra on a Hilbert space H,and φ:A lg L ® A lg L be an additive mapping. This paper proves that if for some positive integer numbers m'n1, φ(am+n+1)=amφ(a)an hold for all a Î A lg L, then there exists some λÎ Z(A lg L)' such thatφ(a)=λa' for all a Î A lg L.

  2. Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization

    DEFF Research Database (Denmark)

    Petersen, B O; Lukas, J; Sørensen, Claus Storgaard;

    1999-01-01

    CDKs. CDC6 interacts specifically with the active Cyclin A/CDK2 complex in vitro and in vivo, but not with Cyclin E or Cyclin B kinase complexes. The cyclin binding domain of CDC6 was mapped to an N-terminal Cy-motif that is similar to the cyclin binding regions in p21(WAF1/SDI1) and E2F-1. The in vivo...... relocalizes to the cytoplasm when Cyclin A/CDK2 is activated. In agreement with CDC6 phosphorylation being specifically mediated by Cyclin A/CDK2, we show that ectopic expression of Cyclin A, but not of Cyclin E, leads to rapid relocalization of CDC6 from the nucleus to the cytoplasm. Based on our data we...... suggest that the phosphorylation of CDC6 by Cyclin A/CDK2 is a negative regulatory event that could be implicated in preventing re-replication during S phase and G2....

  3. Inhibition of CDC25B Phosphatase Through Disruption of Protein-Protein Interaction

    Energy Technology Data Exchange (ETDEWEB)

    Lund, George; Dudkin, Sergii; Borkin, Dmitry; Ni, Wendi; Grembecka, Jolanta; Cierpicki, Tomasz [Michigan

    2015-04-29

    CDC25 phosphatases are key cell cycle regulators and represent very attractive but challenging targets for anticancer drug discovery. Here, we explored whether fragment-based screening represents a valid approach to identify inhibitors of CDC25B. This resulted in identification of 2-fluoro-4-hydroxybenzonitrile, which directly binds to the catalytic domain of CDC25B. Interestingly, NMR data and the crystal structure demonstrate that this compound binds to the pocket distant from the active site and adjacent to the protein–protein interaction interface with CDK2/Cyclin A substrate. Furthermore, we developed a more potent analogue that disrupts CDC25B interaction with CDK2/Cyclin A and inhibits dephosphorylation of CDK2. Based on these studies, we provide a proof of concept that targeting CDC25 phosphatases by inhibiting their protein–protein interactions with CDK2/Cyclin A substrate represents a novel, viable opportunity to target this important class of enzymes.

  4. 75 FR 63844 - Health Resources and Services Administration CDC/HRSA Advisory Committee on HIV and STD...

    Science.gov (United States)

    2010-10-18

    ... CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment (CHACHSPT) In accordance with... Person for More Information: Margie Scott-Cseh, CDC, National Center for HIV/AIDS, Viral Hepatitis,...

  5. Relaciones entre el derecho internacional y el derecho interno: Nuevas perspectivas doctrinales y jurisprudenciales en el ámbito americano

    Directory of Open Access Journals (Sweden)

    Aníbal Quiroga León

    2005-01-01

    Full Text Available El artículo analiza la protección de los derechos fundamentales en el marco de las relaciones entre derecho internacional y derecho interno, teniendo como punto central el sistema interamericano y sus órganos de aplicación, La Comisión y la Corte Interamericana de Derechos Humanos, considerando aspectos procedimentales y la adopción de medidas cautelares por esta última. Finalmente, se analiza la superioridad del derecho internacional de los derechos humanos sobre el derecho interno, teniendo como fundamento los casos "Barrios Altos" (Chumbipoma Aguirre y otros, cuya sentencia es del 14 de marzo del 2001 y el caso "La última tentación de Cristo" (Olmedo Bustos y otros cuya sentencia es del 05 de febrero del 2001.This article analyzes the protection of fundamental rights within the framework of the relationship between international and internal law. The core of our analysis is the Inter-American system, and its bodies of application: the Inter- American Human Rights Commission and Court. We consider procedural dimensions as well as precautionary measures pertaining to the latter. Finally, we examine the preeminence of the international law of human rights over internal law, on grounds of the "Barrios Altos" case (Chumbipoma Aguirre et al., decided on March, 2001 y the "La última tentación de Cristo" case (Olmedo Bustos et al. decided on February 5, 2001.

  6. The small GTPase Cdc42 is necessary for primary ciliogenesis in renal tubular epithelial cells.

    Science.gov (United States)

    Zuo, Xiaofeng; Fogelgren, Ben; Lipschutz, Joshua H

    2011-06-24

    Primary cilia are found on many epithelial cell types, including renal tubular epithelial cells, where they participate in flow sensing. Disruption of cilia function has been linked to the pathogenesis of polycystic kidney disease. We demonstrated previously that the exocyst, a highly conserved eight-protein membrane trafficking complex, localizes to primary cilia of renal tubular epithelial cells, is required for ciliogenesis, biochemically and genetically interacts with polycystin-2 (the protein product of the polycystic kidney disease 2 gene), and, when disrupted, results in MAPK pathway activation both in vitro and in vivo. The small GTPase Cdc42 is a candidate for regulation of the exocyst at the primary cilium. Here, we demonstrate that Cdc42 biochemically interacts with Sec10, a crucial component of the exocyst complex, and that Cdc42 colocalizes with Sec10 at the primary cilium. Expression of dominant negative Cdc42 and shRNA-mediated knockdown of both Cdc42 and Tuba, a Cdc42 guanine nucleotide exchange factor, inhibit ciliogenesis in Madin-Darby canine kidney cells. Furthermore, exocyst Sec8 and polycystin-2 no longer localize to primary cilia or the ciliary region following Cdc42 and Tuba knockdown. We also show that Sec10 directly interacts with Par6, a member of the Par complex that itself directly interacts with Cdc42. Finally, we show that Cdc42 knockdown results in activation of the MAPK pathway, something observed in cells with dysfunctional primary cilia. These data support a model in which Cdc42 localizes the exocyst to the primary cilium, whereupon the exocyst then targets and docks vesicles carrying proteins necessary for ciliogenesis.

  7. The Small GTPase Cdc42 Is Necessary for Primary Ciliogenesis in Renal Tubular Epithelial Cells*

    Science.gov (United States)

    Zuo, Xiaofeng; Fogelgren, Ben; Lipschutz, Joshua H.

    2011-01-01

    Primary cilia are found on many epithelial cell types, including renal tubular epithelial cells, where they participate in flow sensing. Disruption of cilia function has been linked to the pathogenesis of polycystic kidney disease. We demonstrated previously that the exocyst, a highly conserved eight-protein membrane trafficking complex, localizes to primary cilia of renal tubular epithelial cells, is required for ciliogenesis, biochemically and genetically interacts with polycystin-2 (the protein product of the polycystic kidney disease 2 gene), and, when disrupted, results in MAPK pathway activation both in vitro and in vivo. The small GTPase Cdc42 is a candidate for regulation of the exocyst at the primary cilium. Here, we demonstrate that Cdc42 biochemically interacts with Sec10, a crucial component of the exocyst complex, and that Cdc42 colocalizes with Sec10 at the primary cilium. Expression of dominant negative Cdc42 and shRNA-mediated knockdown of both Cdc42 and Tuba, a Cdc42 guanine nucleotide exchange factor, inhibit ciliogenesis in Madin-Darby canine kidney cells. Furthermore, exocyst Sec8 and polycystin-2 no longer localize to primary cilia or the ciliary region following Cdc42 and Tuba knockdown. We also show that Sec10 directly interacts with Par6, a member of the Par complex that itself directly interacts with Cdc42. Finally, we show that Cdc42 knockdown results in activation of the MAPK pathway, something observed in cells with dysfunctional primary cilia. These data support a model in which Cdc42 localizes the exocyst to the primary cilium, whereupon the exocyst then targets and docks vesicles carrying proteins necessary for ciliogenesis. PMID:21543338

  8. Technical Review of SRS Dose Reconstrruction Methods Used By CDC

    Energy Technology Data Exchange (ETDEWEB)

    Simpkins, Ali, A

    2005-07-20

    At the request of the Centers for Disease Control and Prevention (CDC), a subcontractor Advanced Technologies and Laboratories International, Inc.(ATL) issued a draft report estimating offsite dose as a result of Savannah River Site operations for the period 1954-1992 in support of Phase III of the SRS Dose Reconstruction Project. The doses reported by ATL differed than those previously estimated by Savannah River Site SRS dose modelers for a variety of reasons, but primarily because (1) ATL used different source terms, (2) ATL considered trespasser/poacher scenarios and (3) ATL did not consistently use site-specific parameters or correct usage parameters. The receptors with the highest dose from atmospheric and liquid pathways were within about a factor of four greater than dose values previously reported by SRS. A complete set of technical comments have also been included.

  9. Finite element dynamic analysis on CDC STAR-100 computer

    Science.gov (United States)

    Noor, A. K.; Lambiotte, J. J., Jr.

    1978-01-01

    Computational algorithms are presented for the finite element dynamic analysis of structures on the CDC STAR-100 computer. The spatial behavior is described using higher-order finite elements. The temporal behavior is approximated by using either the central difference explicit scheme or Newmark's implicit scheme. In each case the analysis is broken up into a number of basic macro-operations. Discussion is focused on the organization of the computation and the mode of storage of different arrays to take advantage of the STAR pipeline capability. The potential of the proposed algorithms is discussed and CPU times are given for performing the different macro-operations for a shell modeled by higher order composite shallow shell elements having 80 degrees of freedom.

  10. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry

    DEFF Research Database (Denmark)

    Hein, Jamin B; Nilsson, Jakob

    2016-01-01

    Proper cell-cycle progression requires tight temporal control of the Anaphase Promoting Complex/Cyclosome (APC/C), a large ubiquitin ligase that is activated by one of two co-activators, Cdh1 or Cdc20. APC/C and Cdc20 are already present during interphase but APC/C-Cdc20 regulation during...

  11. The Ubx2 and Ubx3 cofactors direct Cdc48 activity to proteolytic and nonproteolytic ubiquitin-dependent processes

    DEFF Research Database (Denmark)

    Hartmann-Petersen, Rasmus; Wallace, Mairi; Hofmann, Kay;

    2004-01-01

    Valosin-containing protein, VCP/p97 or Cdc48, is a eukaryotic ATPase involved in membrane fusion, protein transport, and protein degradation. We describe two proteins, Ubx2 and Ubx3, which interact with Cdc48 in fission yeast. Ubx3 is the ortholog of p47/Shp1, a previously described Cdc48 cofactor...

  12. The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse

    DEFF Research Database (Denmark)

    Vasyutina, Elena; Martarelli, Benedetta; Brakebusch, Cord;

    2009-01-01

    Rac1 and Cdc42 are small G-proteins that regulate actin dynamics and affect plasma membrane protrusion and vesicle traffic. We used conditional mutagenesis in mice to demonstrate that Rac1 and Cdc42 are essential for myoblast fusion in vivo and in vitro. The deficit in fusion of Rac1 or Cdc42...

  13. MODELI SISTEMA POSLUŽIVANJA

    OpenAIRE

    Varga, Mirko

    1989-01-01

    U članku se raspravlja o modelima sistema posluživanja (MSP). Razrađuju se osnovni elementi MSP-a, navodi se primjer i najznačajnije karakteristike. Ukazuje se također na složenost postavljanja odgovarajuće koncepcije upravljanja transakcijama te navode karakteristike najčešćih načina posluživanja. U trećem dijelu komentiramo najčešća obilježja sistema koje obično tretiramo modelom. U četvrtom dijelu navode se ograničenja modela te iz toga proizašla potreba za proširenjem definicije MSP-a. Na...

  14. Nociones del Sistema Tributario Mexicano

    Directory of Open Access Journals (Sweden)

    Froylán Balderas Torres

    2012-01-01

    Full Text Available La presente información se encamina a interiorizar al lector en el tema fiscal con palabras sencillas, describiendo en forma sucinta los antecedentes de los tributos, hasta llegar y describir el sistema impositivo actual mexicano, y de ahí estudiar en forma específica el impuesto que tiene más relevancia por la cantidad que se recauda siendo el Impuesto Sobre la Renta.

  15. O Crisol: periódico das alunas do Colégio Americano (Porto Alegre/RS, 1945-1964 - O Crisol: the jornal by students of the Colégio Americano (Porto Alegre/RS, 1945-1964

    Directory of Open Access Journals (Sweden)

    Dóris Bittencourt Almeida, Brasil

    2013-04-01

    Full Text Available Neste trabalho investiga-se o periódico O Crisol, produzido pelas alunas do Colégio Americano, uma escola de Porto Alegre/RS, entre os anos de 1945 e 1964. Esse periódicodiz muito à História da Educação, pois evidencia saberes e práticas que circulavam nacomunidade do Colégio e revela um pouco das representações da cultura juvenil daquele tempo. Aqui interessam os significados dos discursos difundidos pelo periódico nos processos de subjetivação provocados pelos textos e suas possíveis influências na construção das identidades das jovens leitoras e escritoras do jornal. A pesquisa identifica-se com os pressupostos teóricos da História Cultural e se inscreve no campo das práticas de leitura e escrita e de memórias juvenis, tendo como referenciais as concepções da cultura escrita enquanto produção discursiva de um determinado tempo e lugar.Palavras-chave: memórias juvenis, escritas escolares, práticas de escrita. O CRISOL: THE JORNAL BY STUDENTS OF THE COLÉGIO AMERICANO (PORTO ALEGRE/RS, 1945-1964 AbstractThis work investigates the journalO Crisolproduced by students of the Colégio Americano, a school in Porto Alegre/RS, between the years 1945 and 1964.The journal says much to the History of Education, as evidenced knowledges and pratices circulating in the community,showing a bit of representations of youth culture in that time. Herethe interest by meaning’s dissemination of speeches by the journal in the processes of subjectivation caused by texts and their possible influences on the construction of identities of young readers and writers of the newspaper. The research identifies with the theoreticals presuppositions o Cultural History and inscribed in the field of reading and writing practices and memories of youth, taking as reference the concepts of the writing culture as a discursive production of a determinated time and place.Key-words: youth’s memories, written school, writing practices. O CRISOL: EL

  16. ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization

    Science.gov (United States)

    Bruurs, Lucas J.M.; Donker, Lisa; Zwakenberg, Susan; Zwartkruis, Fried J.; Begthel, Harry; Knisely, A.S.; Posthuma, George; van de Graaf, Stan F.J.; Paulusma, Coen C.

    2015-01-01

    During yeast cell polarization localization of the small GTPase, cell division control protein 42 homologue (Cdc42) is clustered to ensure the formation of a single bud. Here we show that the disease-associated flippase ATPase class I type 8b member 1 (ATP8B1) enables Cdc42 clustering during enterocyte polarization. Loss of this regulation results in increased apical membrane size with scattered apical recycling endosomes and permits the formation of more than one apical domain, resembling the singularity defect observed in yeast. Mechanistically, we show that to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. Disturbing this interaction, either by ATP8B1 depletion or by introduction of a Cdc42 mutant defective in lipid binding, increases Cdc42 mobility and results in apical membrane enlargement. Re-establishing Cdc42 clustering, by tethering it to the apical membrane or lowering its diffusion, restores normal apical membrane size in ATP8B1-depleted cells. We therefore conclude that singularity regulation by Cdc42 is conserved between yeast and human and that this regulation is required to maintain healthy tissue architecture. PMID:26416959

  17. CDC27 protein is involved in radiation response in squamous cell cervix carcinoma.

    Science.gov (United States)

    Rajkumar, T; Gopal, G; Selvaluxmi, G; Rajalekshmy, K R

    2005-10-01

    In the present study, an attempt was made to identify genes involved in radiation response in cervix carcinoma. Differential display technique was used to study the expression profiles of tumour biopsy samples obtained from patients, responding and not responding to treatment. The samples were obtained prior to radiotherapy and subsequent to treatment with Tele-radiation at 10 Gray (Gy). One of the differentially expressed cDNAs, when sequenced was identified to be CDC27. Immuno-histochemical analysis of pre- and post-treated tumour samples from fifteen patients showed the downregulation of expression of CDC27 protein in seven patients. Down-regulation was associated with poorer response to radiotherapy. Cervical cancer cell lines SiHa and C33A were irradiated and their nuclei were stained for expression of CDC27 and analyzed using fluorescent-activated cell sorting (FACS). Down-regulation of CDC27 protein in the irradiated SiHa cell line was associated with greater survival fraction, compared to the irradiated C33A cell line, which had only slight fall in the level of CDC27 protein. This is the first study to suggest a role for CDC27 in radiation response. However, a larger cohort is needed to further confirm the value of CDC27 protein as a predictive marker, for radiation response in cervix cancer.

  18. Opposing Roles for Actin in Cdc42p PolarizationD⃞

    Science.gov (United States)

    Irazoqui, Javier E.; Howell, Audrey S.; Theesfeld, Chandra L.; Lew, Daniel J.

    2005-01-01

    In animal and fungal cells, the monomeric GTPase Cdc42p is a key regulator of cell polarity that itself exhibits a polarized distribution in asymmetric cells. Previous work showed that in budding yeast, Cdc42p polarization is unaffected by depolymerization of the actin cytoskeleton (Ayscough et al., J. Cell Biol. 137, 399–416, 1997). Surprisingly, we now report that unlike complete actin depolymerization, partial actin depolymerization leads to the dispersal of Cdc42p from the polarization site in unbudded cells. We provide evidence that dispersal is due to endocytosis associated with cortical actin patches and that actin cables are required to counteract the dispersal and maintain Cdc42p polarity. Thus, although Cdc42p is initially polarized in an actin-independent manner, maintaining that polarity may involve a reinforcing feedback between Cdc42p and polarized actin cables to counteract the dispersing effects of actin-dependent endocytosis. In addition, we report that once a bud has formed, polarized Cdc42p becomes more resistant to dispersal, revealing an unexpected difference between unbudded and budded cells in the organization of the polarization site. PMID:15616194

  19. Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes

    Science.gov (United States)

    Lange, Bodo M.H.; Rebollo, Elena; Herold, Andrea; González, Cayetano

    2002-01-01

    Cdc37 has been shown to be required for the activity and stability of protein kinases that regulate different stages of cell cycle progression. However, little is known so far regarding interactions of Cdc37 with kinases that play a role in cell division. Here we show that the loss of function of Cdc37 in Drosophila leads to defects in mitosis and male meiosis, and that these phenotypes closely resemble those brought about by the inactivation of Aurora B. We provide evidence that Aurora B interacts with and requires the Cdc37/Hsp90 complex for its stability. We conclude that the Cdc37/Hsp90 complex modulates the function of Aurora B and that most of the phenotypes brought about by the loss of Cdc37 function can be explained by the inactivation of this kinase. These observations substantiate the role of Cdc37 as an upstream regulatory element of key cell cycle kinases. PMID:12374737

  20. La reforma del sistema electoral chileno

    Directory of Open Access Journals (Sweden)

    Mariela Rubano Lapasta

    2007-01-01

    Full Text Available La modificación del sistema electoral público chileno se ha transformado nuevamente en tema de debate público, después de la Reforma Constitucional de 2005. El Gobierno ha propuesto reformar el sistema electoral, sustituirlo por otro que permita a las minorías tener representación en el Congreso. Se abordan brevemente los sistemas electorales en el derecho comparado, en especial el caso chileno, evaluando las ventajas y desventajas del sistema electoral nacional. Se propone recurrir al mecanismo de primarias a fin de elegir a los candidatos. Se concluye que no hay ningún sistema ideal, importa más las características del sistema político y no tanto el método electoral con el cual se eligen a los representantes.

  1. Cdc6 is a rate-limiting factor for proliferative capacity during HL60 cell differentiation

    International Nuclear Information System (INIS)

    The DNA replication (or origin) licensing pathway represents a critical step in cell proliferation control downstream of growth signalling pathways. Repression of origin licensing through down-regulation of the MCM licensing factors (Mcm2-7) is emerging as a ubiquitous route for lowering proliferative capacity as metazoan cells exit the cell division cycle into quiescent, terminally differentiated and senescent 'out-of-cycle' states. Using the HL60 monocyte/macrophage differentiation model system and a cell-free DNA replication assay, we have undertaken direct biochemical investigations of the coupling of origin licensing to the differentiation process. Our data show that down-regulation of the MCM loading factor Cdc6 acts as a molecular switch that triggers loss of proliferative capacity during early engagement of the somatic differentiation programme. Consequently, addition of recombinant Cdc6 protein to in vitro replication reactions restores DNA replication competence in nuclei prepared from differentiating cells. Differentiating HL60 cells over-expressing either wild-type Cdc6 or a CDK phosphorylation-resistant Cdc6 mutant protein (Cdc6A4) exhibit an extended period of cell proliferation compared to mock-infected cells. Notably, differentiating HL60 cells over-expressing the Cdc6A4 mutant fail to down-regulate Cdc6 protein levels, suggesting that CDK phosphorylation of Cdc6 is linked to its down-regulation during differentiation and the concomitant decrease in cell proliferation. In this experimental model, Cdc6 therefore plays a key role in the sequential molecular events leading to repression of origin licensing and loss of proliferative capacity during execution of the differentiation programme

  2. Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Ting; Wang, Wei-Na, E-mail: weina63@aliyun.com; Gu, Mei-Mei; Xie, Chen-Ying; Xiao, Yu-Chao; Liu, Yuan; Wang, Lei

    2015-06-15

    Highlights: • Cd{sup 2+} induces Cdc42 and MAPKs pathway related gene of Litopenaeus vannamei up-regulation. • Reduction of THC, increase of ROS production and apoptotic cell rate were observed when the shrimps exposure to Cd{sup 2+}. • DsRNA-suppression of LvCdc42 and MAPKs during Cd{sup 2+} stress reduces the ROS production and apoptosis. • We conclude that LvCdc42 and MAPKs play key roles in Cd{sup 2+} stress responses of shrimps. - Abstract: Cadmium, one of the most toxic heavy metals in aquatic environments, has severe effects on marine invertebrates and fishes. The MAPK signaling pathway plays a vital role in stress responses of animals. The mitogen-activated protein kinase (MAPK) signaling pathway plays a vital role in animals’ stress responses, including mediation of apoptosis induced by the Rho GTPase Cdc42. However, there is limited knowledge about its function in shrimps, although disorders exacerbated by environmental stresses (including heavy metal pollution) have caused serious mortality in commercially cultured shrimps. Thus, we probed roles of Cdc42 in Litopenaeus vannamei shrimps (LvCdc42) during cadmium exposure by inhibiting its expression using dsRNA-mediated RNA interference. The treatment successfully reduced expression levels of MAPKs (including p38, JNK, and ERK). Cadmium exposure induced significant increases in expression levels of LvCdc42 and MAPKs, accompanied by reductions in total hemocyte counts (THC) and increases in apoptotic hemocyte ratios and ROS production. However, all of these responses were much weaker in LvCdc42-suppressed shrimps, in which mortality rates were higher than in controls. Our results suggest that the MAPK pathway plays a vital role in shrimps’ responses to Cd{sup 2+}. They also indicate that LvCdc42 in shrimps participates in its regulation, and thus plays key roles in ROS production, regulation of apoptosis and associated stress responses.

  3. Poslovanje in financiranje zdravstvenega sistema Republike Slovenije

    OpenAIRE

    Gorup, Luka

    2016-01-01

    V nalogi smo podrobneje predstavili zdravstveni sistem Republike Slovenije, ureditev obveznega zdravstvenega zavarovanja in drugih virov, s katerimi financiramo zdravstveni sistem. Preučili smo poslovanje sistema, plačevanje zdravstvenih izvajalcev in zaposlenih ter predstavili partnerje pri upravljanju sistema. Natačneje smo predstavili porazdelitev in ureditev različnih ravni zdravstvenega sistema, razporeditev in organiziranost človeških, finančnih, materialnih in drugih virov. Sis...

  4. Cytoplasmic Localization of Human cdc25C during Interphase Requires an Intact 14-3-3 Binding Site

    OpenAIRE

    Dalal, Sorab N.; Schweitzer, Colleen M.; Gan, Jianmin; DeCaprio, James A.

    1999-01-01

    cdc25C induces mitosis by activating the cdc2-cyclin B complex. The intracellular localization of cyclin B1 is regulated in a cell cycle-specific manner, and its entry into the nucleus may be required for the initiation of mitosis. To determine the cellular localization of cdc25C, monoclonal antibodies specific for cdc25C were developed and used to demonstrate that in human cells, cdc25C is retained in the cytoplasm during interphase. A deletion analysis identified a 58-amino-acid region (ami...

  5. El visón americano Neovison vison (Schreber 1777 en España: posibles causas de su expansión e interacción con otros mamíferos semiacuáticos

    Directory of Open Access Journals (Sweden)

    A. Balmori

    2015-01-01

    Full Text Available El establecimiento de poblaciones de visón americano asilvestradas en España data de la década de los 60 del siglo XX. Los individuos procedentes de escapes de granjas peleteras han establecido poblaciones asilvestradas que se han expandido rápidamente por la cuenca del Duero. Tanto los motivos de su exitosa expansión en la submeseta norte como los efectos en la comunidad de vertebrados ligados a los ríos son poco conocidos. En este trabajo se revisan las posibles causas del éxito colonizador del visón americano en España y el previsible impacto sobre las especies de mamíferos semiacuáticos con las que comparte territorio, con especial mención de aquellos cuyas poblaciones pueden verse amenazadas. Se proponen las actuaciones necesarias para favorecer la recuperación de las poblaciones de visón europeo y frenar la expansión del visón americano en la Cuenca del Duero. La evolución de la superficie de ocupación del visón europeo permite albergar cierto optimismo.

  6. NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling

    DEFF Research Database (Denmark)

    Sørensen, Claus Storgaard; Melixetian, Marina; Klein, Ditte Kjaersgaard;

    2010-01-01

    The DNA damage induced G(2)/M checkpoint is an important guardian of the genome that prevents cell division when DNA lesions are present. The checkpoint prevents cells from entering mitosis by degrading CDC25A, a key CDK activator. CDC25A proteolysis is controlled by direct phosphorylation events...... is required for beta-TrCP mediated CDC25A polyubiquitylation and degradation. The activity of NEK11 is in turn controlled by CHK1 that activates NEK11 via phosphorylation on serine 273. Since inhibition of NEK11 activity forces checkpoint-arrested cells into mitosis and cell death, NEK11 is, like CHK1...

  7. Cdc20 mediates D-box-dependent degradation of Sp100

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Ran; Li, Ke-min; Zhou, Cai-hong; Xue, Jing-lun [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China); Ji, Chao-neng, E-mail: Chnji@fudan.edu.cn [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China); Chen, Jin-zhong, E-mail: kingbellchen@fudan.edu.cn [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China)

    2011-12-02

    Highlights: Black-Right-Pointing-Pointer Cdc20 is a co-activator of APC/C complex. Black-Right-Pointing-Pointer Cdc20 recruits Sp100 and mediates its degradation. Black-Right-Pointing-Pointer The D-box of Sp100 is required for Cdc20-mediated degradation. Black-Right-Pointing-Pointer Sp100 expresses consistently at both the mRNA and protein levels in cell cycle. -- Abstract: Cdc20 is a co-activator of the anaphase-promoting complex/cyclosome (APC/C complex), which recruits substrates at particular phases of the cell cycle and mediates their degradation. Sp100 is a PML-NB scaffold protein, which localizes to nuclear particles during interphase and disperses from them during mitosis, participates in viral resistance, transcriptional regulation, and apoptosis. However, its metabolism during the cell cycle has not yet been fully characterized. We found a putative D-box in Sp100 using the Eukaryotic Linear Motif (ELM) predictor database. The putative D-box of Sp100 was verified by mutational analysis. Overexpression of Cdc20 resulted in decreased levels of both endogenous Sp100 protein and overexpressed Sp100 mRNA in HEK 293 cells. Only an overexpressed D-box deletion mutant of Sp100 accumulated in HEK293 cells that also overexpressed Cdc20. Cdc20 knockdown by cdc20 specific siRNA resulted in increased Sp100 protein levels in cells. Furthermore, we discovered that the Cdc20 mediated degradation of Sp100 is diminished by the proteasome inhibitor MG132, which suggests that the ubiquitination pathway is involved in this process. However, unlike the other Cdc20 substrates, which display oscillating protein levels, the level of Sp100 protein remains constant throughout the cell cycle. Additionally, both overexpression and knockdown of endogenous Sp100 had no effect on the cell cycle. Our results suggested that sp100 is a novel substrate of Cdc20 and it is degraded by the ubiquitination pathway. The intact D-box of Sp100 was necessary for this process. These findings expand

  8. CDC25A governs proliferation and differentiation of FLT3-ITD acute myeloid leukemia

    OpenAIRE

    Bertoli, Sarah; Boutzen, Helena; David, Laure; Larrue, Clément; Vergez, François; Fernandez-Vidal, Anne; Yuan, Lingli; Hospital, Marie-Anne; Tamburini, Jérôme; Demur, Cecile; Delabesse, Eric; Saland, Estelle; Sarry, Jean-Emmanuel; Galcera, Marie-Odile; Mansat-De Mas, Véronique

    2015-01-01

    We investigated cell cycle regulation in acute myeloid leukemia cells expressing the FLT3-ITD mutated tyrosine kinase receptor, an underexplored field in this disease. Upon FLT3 inhibition, CDC25A mRNA and protein were rapidly down-regulated, while levels of other cell cycle proteins remained unchanged. This regulation was dependent on STAT5, arguing for FLT3-ITD-dependent transcriptional regulation of CDC25A. CDC25 inhibitors triggered proliferation arrest and cell death of FLT3-ITD as well ...

  9. La reforma del sistema electoral chileno

    OpenAIRE

    Mariela Rubano Lapasta

    2007-01-01

    La modificación del sistema electoral público chileno se ha transformado nuevamente en tema de debate público, después de la Reforma Constitucional de 2005. El Gobierno ha propuesto reformar el sistema electoral, sustituirlo por otro que permita a las minorías tener representación en el Congreso. Se abordan brevemente los sistemas electorales en el derecho comparado, en especial el caso chileno, evaluando las ventajas y desventajas del sistema electoral nacional. Se propone ...

  10. As contribuições do II Congresso Médico Latino-Americano para a enfermagem no Brasil Las Contribuciones del II Congreso Médico Latino-Americano para la enfermería en el Brasil The contributions of II Latin American Medical Congress for the Nursing in Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda Teles Morais

    2009-03-01

    Full Text Available Estudo sobre as recomendações do II Congresso Médico Latino-Americano, em 1904, para a organização da Enfermagem no Brasil. Objetivos: Caracterizar os médicos brasileiros que participaram do II Congresso Médico Latino-Americano e analisar as recomendações deste Congresso para a Enfermagem. Trata-se de um estudo histórico-social, embasado na análise documental. Resultados: A participação de médicos brasileiros neste evento foi grande. Em meio às discussões de ordem sanitária, figurou a questão da criação de escolas de enfermeiras, apresentada pelo médico Moisés Amaral. Diante das evidências da eficácia de se ter uma enfermeira instruída em escolas próprias, e de acordo com os preceitos da medicina moderna, foi aprovada a proposta para a criação de escolas de enfermagem. Apesar da posição estratégica que os representantes brasileiros nesse Congresso ocupavam no país, não encontramos evidências do empenho deles em trabalhar efetivamente para a criação de escolas de enfermeiras como uma estratégia para enfrentar os problemas sanitários.Estudio sobre las recomendaciones del II Congreso Médico Latino-Americano, en 1904, para la organización de la enfermería el Brasil. Objetivos: Caracterizar los médicos brasileños que participaran del II Congreso Médico Latino-Americano; y analizar las recomendaciones de este congreso para la enfermería. Estudio histórico-social basado en la análisis documental. Resultados: la participación brasileña en este acontecimiento foi grande. En medio a las discusiones sanitarias, figuró la cuestión de creación de escuelas de enfermeríapresentada por el médico Moisés Amaral. Frente a las evidencias de la eficacia de tener una enfermera instruida en escuelas apropiadas y de acuerdo con los preceptos de la medicina moderna fue aprobada la propuesta de creación de escuelas de enfermería. A pesar de la estratégica posición de los representantes brasileños en ese Congreso

  11. O pensamento conservador ibero-americano na era das independências (1808-1850 Conservative ibero-american thought in the age of independences (1808-1850

    Directory of Open Access Journals (Sweden)

    Christian Edward Cyril Lynch

    2008-01-01

    Full Text Available A intenção do artigo é fornecer um panorama ideológico da América Ibérica da época das independências, especialmente no que diz respeito às ideologias de direita. Busca-se, assim, definir o que seja conservadorismo, distinguindo-o do puro reacionarismo para, em seguida, comparar a orientação de José Bonifácio e de Marquês de Caravelas, no Brasil, com aquela desenvolvida por Bolívar, tendo em vista os dilemas do conservadorismo ibero-americano. A comparação esclarece as diferentes trajetórias da antiga América portuguesa e da antiga América hispânica, no decorrer do século XIX.This article intends to study the right-wing ideologies of Latin America during the first half of the nineteenth century. At first, it tries to define what conservatism is and to point out its differences from the rhetoric of reaction; later, the thought of José Bonifacio and the Marquis of Caravelas is compared to that of Simon Bolivar, underlying the dilemmas of Latin American conservatism. The comparison enlightens the different ways followed by the ancient Portuguese and Hispanic America after political independence.

  12. La Organización de Estados Americanos y su incidencia democrática post Trujillo en República Dominicana (1961-1965

    Directory of Open Access Journals (Sweden)

    Mario J. Gallego Cosme

    2014-07-01

    Full Text Available Este artículo es una reflexión acerca del convulso pe- ríodo comprendido entre el final del régimen del dictador Trujillo y el final de la Guerra Civil en República Domi- nicana. En este recuento de la historia de este pequeño país caribeño, se revisarán las relaciones de su política exterior con sus principales acontecimientos domésticos. En este sentido se prestará especial atención al importan- te papel de la Organización de Estados Americanos y de los Estados Unidos, ambos actores preponderantes con incidencia en el devenir de las siguientes décadas para los dominicanos. AbstractThis paper focuses on a reflection on the turbulent period between the end of Trujillo’s dictatorial regime and the end of the Civil War in Dominican Republic. Through a historical approach of this period of time in this little Caribbean country, issues regarding the Do- minican foreign policies will be reviewed to link them with major events taking place at domestic level. In this respect, special attention will be provided to the import- ant role of the Organization of American States and the United States, both actors with preponderant influence on setting the course for political actions in the following decades in this country’s history. 

  13. Nodulação em cultivares de feijão dos conjuntos gênicos andino e meso-americano

    Directory of Open Access Journals (Sweden)

    Franco Marília Caixeta

    2002-01-01

    Full Text Available O objetivo deste trabalho foi determinar a capacidade de nodulação e a especificidade de feijão (Phaseolus vulgaris L. dos conjuntos gênicos andino e meso-americano submetidos a inóculo de Rhizobium. O experimento foi estabelecido em parcelas subdivididas em blocos ao acaso, com quatorze cultivares de feijão e três estirpes de Rhizobium (R. etli KIM 5, R. etli CIAT 632 e R. tropici CIAT 899. Somente as cultivares andinas WAF 15, WAF 7, Mineiro Precoce, WAF 6 e Antioquia 8 apresentaram especificidade na nodulação. Em relação à massa seca dos nódulos, houve diferenças significativas dos tratamentos de inoculação nas cultivares andinas WAF 15, WAF 7, WAF 6 e Diacol Andino, e na cultivar meso-americana Ouro Negro. Nenhuma das cultivares restringiu a nodulação, embora tenham sido verificadas diferenças de até 53 e 103 vezes no número e massa de nódulos por planta, respectivamente. Considerando todas as cultivares e estirpes de rizóbio, WAF 15 foi a cultivar com melhor desempenho em número e massa nodular. WAF 6 foi a cultivar de pior desempenho, chegando quase ao nível de restrição da nodulação com a estirpe R. etli CIAT 632.

  14. Institucionalização do Sistema Interamericano de Combate às Drogas: Iniciativas e Progressos

    Directory of Open Access Journals (Sweden)

    Matheus Bernardes Rachadel

    2013-11-01

    Full Text Available http://dx.doi.org/10.5007/1806-5023.2013v10n2p25 O presente artigo analisa o tratamento do fenômeno das drogas na América Latina, discutindo os avanços na criação de um sistema continental de combate às drogas, representado pela Comissão Interamericana para o Controle do Abuso de Drogas (CICAD. A primeira parte do trabalho traça uma retrospectiva da criação da CICAD como iniciativa no surgimento de um arcabouço institucional e legal de tratamento do tema das drogas nas Américas. A segunda parte aborda o processo de consolidação da CICAD como instituição central nesse regime, focada em ultrapassar os métodos tradicionais de enfrentamento do fenômeno. Conclui-se que, ao longo da segunda metade do século XX, os países latino-americanos reagiram diante do fenômeno das drogas e que a criação de um regime interamericano para o tema foi fundamental para o fomento da cooperação regional nessa problemática.

  15. Sistemas distribuidos de tiempo real

    OpenAIRE

    Ramón, Hugo Dionisio; Romero, Fernando; Pesado, Patricia Mabel; Bertone, Rodolfo Alfredo; Boracchia, Marcos; Thomas, Pablo Javier; Esponda, Silvia

    2004-01-01

    El objetivo de esta línea de investigación es el estudio y desarrollo de sistemas de software que poseen restricciones temporales. Resulta de interés el estudio de diferentes metodologías para la especificación y desarrollo de aplicaciones en tiempo real, así como las plataformas donde dichas aplicaciones pueden ejecutarse. Se trabaja experimentalmente con distintos tipos de hardware y software entre los que pueden mencionarse placas A/D, mini-robots, máquinas multimicroprocesadores y dife...

  16. Sistemas distribuidos de tiempo real

    OpenAIRE

    Ramón, Hugo Dionisio; Romero, Fernando; González, Alejandro Héctor; Novarini, Pablo; Estrebou, César Armando

    2005-01-01

    El objetivo de esta línea de investigación es el estudio y desarrollo de sistemas de software que poseen restricciones temporales. Resulta de interés el estudio de diferentes metodologías para la especificación y desarrollo de aplicaciones en tiempo real, así como las plataformas donde dichas aplicaciones pueden ejecutarse. Se trabaja experimentalmente con distintos tipos de hardware y software entre los que pueden mencionarse placas A/D, mini-robots, plataformas de hardware (PC, PIC, etc....

  17. Mobiliario urbano: sistema de bancos

    OpenAIRE

    Vallati, Emiliano

    2012-01-01

    Para desarrollar el presente proyecto se partió de considerar al mobiliario urbano como todos los elementos que forman parte del paisaje de la ciudad y que tienen la finalidad de ser útiles para el ciudadano. En este caso, se diseñó un sistema de bancos que interactúan entre sí y que permite incorporar más piezas según el uso, las funciones o las necesidades que se quieran satisfacer. El objetivo fue resolver el problema de sentarse. Para ello, se partió de la acción de sentarse, y se la p...

  18. Argentina en el sistema internacional

    OpenAIRE

    Tini, María Natalia

    2002-01-01

    El presente trabajo apunta a analizar cuál es el lugar "real" que ocupa la Argentina en el Sistema Internacional, en contraste, con el lugar "ideal" en que lo ha ido posicionando nuestra dirigencia en los últimos 10 años. Para ello, es esencial realizar un breve repaso de la política exterior llevada a cabo por las administraciones Menem y De La Rúa, como sustento empírico de dicho análisis. La hipótesis plantea que para proyectar una adecuada política exterior es necesario formular un ...

  19. Characterization of a Dual CDC7/CDK9 Inhibitor in Multiple Myeloma Cellular Models

    International Nuclear Information System (INIS)

    Two key features of myeloma cells are the deregulation of the cell cycle and the dependency on the expression of the BCL2 family of anti-apoptotic proteins. The cell division cycle 7 (CDC7) is an essential S-phase kinase and emerging CDC7 inhibitors are effective in a variety of preclinical cancer models. These compounds also inhibit CDK9 which is relevant for MCL-1 expression. The activity and mechanism of action of the dual CDC7/CDK9 inhibitor PHA-767491 was assessed in a panel of multiple myeloma cell lines, in primary samples from patients, in the presence of stromal cells and in combination with drugs used in current chemotherapeutic regimens. We report that in all conditions myeloma cells undergo cell death upon PHA-767491 treatment and we report an overall additive effect with melphalan, bortezomib and doxorubicin, thus supporting further assessment of targeting CDC7 and CDK9 in multiple myeloma

  20. Characterization of a Dual CDC7/CDK9 Inhibitor in Multiple Myeloma Cellular Models

    Energy Technology Data Exchange (ETDEWEB)

    Natoni, Alessandro [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Coyne, Mark R. E. [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Department of Medicine, National University of Ireland Galway, Galway (Ireland); Department of Haematology, Galway University Hospital, Galway (Ireland); Jacobsen, Alan; Rainey, Michael D.; O’Brien, Gemma; Healy, Sandra [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland); Montagnoli, Alessia; Moll, Jürgen [Nerviano Medical Sciences S.r.l., Via Pasteur 10, Nerviano 20014 (Italy); O’Dwyer, Michael, E-mail: michael.odwyer@nuigalway.ie [Department of Medicine, National University of Ireland Galway, Galway (Ireland); Department of Haematology, Galway University Hospital, Galway (Ireland); Santocanale, Corrado, E-mail: michael.odwyer@nuigalway.ie [Centre for Chromosome Biology, School of Natural Sciences National University of Ireland Galway, Galway (Ireland)

    2013-07-24

    Two key features of myeloma cells are the deregulation of the cell cycle and the dependency on the expression of the BCL2 family of anti-apoptotic proteins. The cell division cycle 7 (CDC7) is an essential S-phase kinase and emerging CDC7 inhibitors are effective in a variety of preclinical cancer models. These compounds also inhibit CDK9 which is relevant for MCL-1 expression. The activity and mechanism of action of the dual CDC7/CDK9 inhibitor PHA-767491 was assessed in a panel of multiple myeloma cell lines, in primary samples from patients, in the presence of stromal cells and in combination with drugs used in current chemotherapeutic regimens. We report that in all conditions myeloma cells undergo cell death upon PHA-767491 treatment and we report an overall additive effect with melphalan, bortezomib and doxorubicin, thus supporting further assessment of targeting CDC7 and CDK9 in multiple myeloma.

  1. Working at a Ferranti-Argus graphics display linked to a CDC 6600

    CERN Multimedia

    1975-01-01

    The photo shows a Ferranti-Argus graphics display linked to a CDC 6600 computer running one of the first interactive graphics packages for physics analysis. The people around are Jean-Claude Marin, Harry Renshall and Emilio Pagiola (right).

  2. Kids 14 and Younger Only Need 2 HPV Vaccine Shots: CDC

    Science.gov (United States)

    ... html Kids 14 and Younger Only Need 2 HPV Vaccine Shots: CDC But agency's revised guidelines still recommend ... and younger require only two doses of the HPV vaccine rather than the previously recommended three shots, U.S. ...

  3. 78 FR 60876 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2013-10-02

    ... the next day. Number for Technical Support: (404) 639-3737 The deadline for notification of attendance...: Carmen Villar, MSW, Designated Federal Officer, Advisory Committee to the Director, CDC, 1600...

  4. Fewer U.S. Kids Die from Abusive Head Trauma: CDC

    Science.gov (United States)

    ... https://medlineplus.gov/news/fullstory_159052.html Fewer U.S. Kids Die From Abusive Head Trauma: CDC Parent ... two years of the study, according to the U.S. Centers for Disease Control and Prevention. Experts said ...

  5. U.S. Suicide Rate Up 24 Percent Since 1999: CDC

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_158462.html U.S. Suicide Rate Up 24 Percent Since 1999: CDC ... per 100,000 people, said researchers from the U.S. Centers for Disease Control and Prevention's National Center ...

  6. SIMWEST: A simulation model for wind and photovoltaic energy storage systems (CDC user's manual), volume 1

    Science.gov (United States)

    Warren, A. W.; Esinger, A. W.

    1979-01-01

    Procedures are given for using the SIMWEST program on CDC 6000 series computers. This expanded software package includes wind and/or photovoltaic systems utilizing any combination of five types of storage (pumped hydro, battery, thermal, flywheel, and pneumatic).

  7. Sistema de apoyo al aprendizaje diagnóstico: de endodiag a un sistema multiagente

    OpenAIRE

    Casali, Ana; Corti, Rosa Marina; D'Agostino, Estela; Biga, Adrián; Siragusa, Martha; Aciar, Silvana

    2005-01-01

    En este trabajo se describe el desarrollo y evolución, a través de distintos prototipos, de un sistema de apoyo a la enseñanza diagnóstica en Endodoncia. Este sistema tiene como objetivo reforzar el conocimiento que adquieren los estudiantes a través de la práctica clínica. Los dos primeros prototipos de este sistema se han desarrollado para ser ejecutados por los alumnos en una sola Terminal. El primero de estos sistemas es EndoDiag, un sistema experto que acompaña al alumno en el diagnóstic...

  8. CDC25B overexpression stabilises centrin 2 and promotes the formation of excess centriolar foci.

    Directory of Open Access Journals (Sweden)

    Rose Boutros

    Full Text Available CDK-cyclin complexes regulate centriole duplication and microtubule nucleation at specific cell cycle stages, although their exact roles in these processes remain unclear. As the activities of CDK-cyclins are themselves positively regulated by CDC25 phosphatases, we investigated the role of centrosomal CDC25B during interphase. We report that overexpression of CDC25B, as is commonly found in human cancer, results in a significant increase in centrin 2 at the centrosomes of interphase cells. Conversely, CDC25B depletion causes a loss of centrin 2 from the centrosome, which can be rescued by treatment with the proteasome inhibitor MG132. CDC25B overexpression also promotes the formation of excess centrin 2 "foci". These foci can accumulate other centrosome proteins, including γ-tubulin and PCM-1, and can function as microtubule organising centres, indicating that these represent functional centrosomes. Formation of centrin 2 foci can be blocked by specific inhibition of CDK2 but not CDK1. CDK2-mediated phosphorylation of Monopolar spindle 1 (Mps1 at the G1/S transition is essential for the initiation of centrosome duplication, and Mps1 is reported to phosphorylate centrin 2. Overexpression of wild-type or non-degradable Mps1 exacerbated the formation of excess centrin 2 foci induced by CDC25B overexpression, while kinase-dead Mps1 has a protective effect. Together, our data suggest that CDC25B, through activation of a centrosomal pool of CDK2, stabilises the local pool of Mps1 which in turn regulates the level of centrin 2 at the centrosome. Overexpression of CDC25B may therefore contribute to tumourigenesis by perturbing the natural turnover of centrosome proteins such as Mps1 and centrin 2, thus resulting in the de novo assembly of extra-numerary centrosomes and potentiating chromosome instability.

  9. An adolescent case of familial hyperparathyroidism with a germline frameshift mutation of the CDC73 gene

    OpenAIRE

    Takeuchi, Takako; Yoto, Yuko; Tsugawa, Takeshi; Kamasaki, Hotaka; Kondo, Atsushi; OGINO, JIRO; Hasegawa, Tadashi; Yama, Naoya; Anan, Sawa; Uchino, Shinya; Ishikawa, Aki; Sakurai, Akihiro; Tsutsumi, Hiroyuki

    2015-01-01

    Abstract. A 13-yr-old boy who complained of persistent nausea, vomiting and weight loss had hypercalcemia and an elevated intact PTH level. Computed tomography confirmed two tumors in the thyroid gland. The tumors were surgically removed and pathologically confirmed as parathyroid adenoma. Because his maternal aunt and grandmother both had histories of parathyroid tumors, genetic investigation was undertaken for him, and a germline frameshift mutation of the CDC73 gene was identified. CDC73 g...

  10. Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens.

    OpenAIRE

    Funke, G; Lucchini, G M; Pfyffer, G E; Marchiani, M; von Graevenitz, A

    1993-01-01

    Fifteen strains of CDC group 1 coryneform and biochemically similar bacteria were isolated from clinical specimens. Of the 15 strains isolated, 11 were derived from abscesses and purulent lesions, mostly from the upper part of the body, and 3 were grown from blood cultures. Nine strains were associated with mixed anaerobic but no other aerobic flora. Seven strains exhibited the classical biochemical profile of CDC coryneform group 1; however, eight strains were unable to reduce nitrate and we...

  11. Human Cdc14A regulates Wee1 stability by counteracting CDK-mediated phosphorylation

    OpenAIRE

    Ovejero, Sara; Ayala, Patricia; Bueno, Avelino; Sacristán, María P.

    2012-01-01

    The activity of Cdk1–cyclin B1 mitotic complexes is regulated by the balance between the counteracting activities of Wee1/Myt1 kinases and Cdc25 phosphatases. These kinases and phosphatases must be strictly regulated to ensure proper mitotic timing. One masterpiece of this regulatory network is Cdk1, which promotes Cdc25 activity and suppresses inhibitory Wee1/Myt1 kinases through direct phosphorylation. The Cdk1-dependent phosphorylation of Wee1 primes phosphorylation by additional kinases s...

  12. Glucose-stimulated Cdc42 Signaling Is Essential for the Second Phase of Insulin Secretion*

    OpenAIRE

    Wang, Zhanxiang; Oh, Eunjin; Thurmond, Debbie C.

    2007-01-01

    The small Rho family GTPases Cdc42 and Rac1 have each been shown to function in insulin exocytosis and are presumed to function in actin remodeling and insulin granule mobilization. However, whether either GTPase is required for the mobilization phase of insulin release (second phase) and are linked in a common signaling pathway has remained unknown. Here we demonstrate that small interfering RNA-mediated depletion of Cdc42 from isolated islets results in the selective loss of second-phase in...

  13. Agricultura Apoiada pela Comunidade: poderia a experiência dos agricultores americanos ser útil para os agricultores urbanos brasileiros? Community Supported Agriculture: could the experience of American farmers be useful to Brazilian urban farmers?

    Directory of Open Access Journals (Sweden)

    M Castelo Branco

    2011-03-01

    Full Text Available O projeto "Agricultura Apoiada pela Comunidade" (AAC é uma alternativa de mercado utilizada na Europa e nos EUA. Neste mercado alternativo, os produtores oferecem a sua produção aos consumidores, sob a forma de cestas de alimentos, alguns meses antes de iniciarem a produção de suas culturas. Algum tempo depois, durante alguns meses, os consumidores irão receber os produtos. Atualmente, diversos países têm uma parcela significativa da sua população vivendo na pobreza. Por isso, diversas políticas públicas foram desenhadas nesses países com o objetivo de reduzi-la. O estímulo à agricultura urbana é uma dessas políticas. No entanto, frequentemente, os produtores urbanos pobres têm dificuldades para adquirir os insumos necessários para manter a sua produção. O apoio contínuo do governo local associado ao apoio contínuo da comunidade local poderia ajudar esses produtores a manter o seu sistema de produção. E, para esses agricultores urbanos pobres, o projeto AAC poderia ser um meio alternativo de organização. O objetivo desse trabalho foi obter algumas informações sobre o projeto AAC nos EUA e avaliar se consumidores e produtores urbanos pobres de uma cidade brasileira de baixa renda aceitariam se engajar neste tipo de projeto. Os produtores americanos envolvidos no projeto AAC em geral cultivavam uma área inferior a 2 ha, ofereciam diferentes tipos de hortaliças aos consumidores por pelo menos quarto meses e geralmente usavam práticas de produção orgânica. No entanto, havia dificuldades para a manutenção do projeto, sendo o planejamento da produção e a dificuldade de sua manutenção ao longo do tempo os maiores problemas. Alguns consumidores da cidade brasileira pesquisada se mostraram dispostos a participar deste tipo de projeto principalmente pela possibilidade de receberem alimentos frescos e produzidos organicamente. No entanto, o mesmo não ocorreu com os produtores urbanos pobres da cidade. E a raz

  14. Sistema de videovigilancia utilizando recursos de LAN

    OpenAIRE

    Fernández García, David

    2007-01-01

    El objetivo principal de este proyecto es realizar un sistema de información que sea capaz de efectuar video vigilancia. Este sistema de información está formado por un servidor y un número, en principio, limitado de clientes

  15. A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation

    Directory of Open Access Journals (Sweden)

    Bernadette Connors

    2014-01-01

    Full Text Available Regulation of DNA repair can be achieved through ubiquitin-mediated degradation of transiently induced proteins. In Saccharomyces cerevisiae, Rad4 is involved in damage recognition during nucleotide excision repair (NER and, in conjunction with Rad23, recruits other proteins to the site of damage. We identified a synthetic interaction upon UV exposure between Rad4 and Cdc20, a protein that modulates the activity of the anaphase promoting complex (APC/C, a multisubunit E3 ubiquitin ligase complex. The moderately UV sensitive Δrad4 strain became highly sensitive when cdc20-1 was present, and was rescued by overexpression of CDC20. The double mutant is also deficient in elicting RNR3-lacZ transcription upon exposure to UV irradiation or 4-NQO compared with the Δrad4 single mutant. We demonstrate that the Δrad4/cdc20-1 double mutant is defective in double strand break repair by way of a plasmid end-joining assay, indicating that Rad4 acts to ensure that damaged DNA is repaired via a Cdc20-mediated mechanism. This study is the first to present evidence that Cdc20 may play a role in the degradation of proteins involved in nucleotide excision repair.

  16. Cdc7p-Dbf4p regulates mitotic exit by inhibiting Polo kinase.

    Directory of Open Access Journals (Sweden)

    Charles T Miller

    2009-05-01

    Full Text Available Cdc7p-Dbf4p is a conserved protein kinase required for the initiation of DNA replication. The Dbf4p regulatory subunit binds Cdc7p and is essential for Cdc7p kinase activation, however, the N-terminal third of Dbf4p is dispensable for its essential replication activities. Here, we define a short N-terminal Dbf4p region that targets Cdc7p-Dbf4p kinase to Cdc5p, the single Polo kinase in budding yeast that regulates mitotic progression and cytokinesis. Dbf4p mediates an interaction with the Polo substrate-binding domain to inhibit its essential role during mitosis. Although Dbf4p does not inhibit Polo kinase activity, it nonetheless inhibits Polo-mediated activation of the mitotic exit network (MEN, presumably by altering Polo substrate targeting. In addition, although dbf4 mutants defective for interaction with Polo transit S-phase normally, they aberrantly segregate chromosomes following nuclear misorientation. Therefore, Cdc7p-Dbf4p prevents inappropriate exit from mitosis by inhibiting Polo kinase and functions in the spindle position checkpoint.

  17. Roles of Aspergillus nidulans Cdc42/Rho GTPase regulators in hyphal morphogenesis and development.

    Science.gov (United States)

    Si, Haoyu; Rittenour, William R; Harris, Steven D

    2016-01-01

    The Rho-related family of GTPases are pivotal regulators of morphogenetic processes in diverse eukaryotic organisms. In the filamentous fungi two related members of this family, Cdc42 and Rac1, perform particularly important roles in the establishment and maintenance of hyphal polarity. The activity of these GTPases is tightly controlled by two sets of regulators: guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Despite the importance of Cdc42 and Rac1 in polarized hyphal growth, the morphogenetic functions of their cognate GEFs and GAPs have not been widely characterized in filamentous fungi outside the Saccharomycotina. Here we present a functional analysis of the Aspergillus nidulans homologs of the yeast GEF Cdc24 and the yeast GAP Rga1. We show that Cdc24 is required for the establishment of hyphal polarity and localizes to hyphal tips. We also show that Rga1 is necessary for the suppression of branching in developing conidiophores. During asexual development Rga1 appears to act primarily via Cdc42 and in doing so serves as a critical determinant of conidiophore architecture. Our results provide new insight into the roles of Cdc42 during development in A nidulans. PMID:26932184

  18. CDC Guideline for Prescribing Opioids for Chronic Pain - United States, 2016.

    Science.gov (United States)

    Dowell, Deborah; Haegerich, Tamara M; Chou, Roger

    2016-01-01

    This guideline provides recommendations for primary care clinicians who are prescribing opioids for chronic pain outside of active cancer treatment, palliative care, and end-of-life care. The guideline addresses 1) when to initiate or continue opioids for chronic pain; 2) opioid selection, dosage, duration, follow-up, and discontinuation; and 3) assessing risk and addressing harms of opioid use. CDC developed the guideline using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) framework, and recommendations are made on the basis of a systematic review of the scientific evidence while considering benefits and harms, values and preferences, and resource allocation. CDC obtained input from experts, stakeholders, the public, peer reviewers, and a federally chartered advisory committee. It is important that patients receive appropriate pain treatment with careful consideration of the benefits and risks of treatment options. This guideline is intended to improve communication between clinicians and patients about the risks and benefits of opioid therapy for chronic pain, improve the safety and effectiveness of pain treatment, and reduce the risks associated with long-term opioid therapy, including opioid use disorder, overdose, and death. CDC has provided a checklist for prescribing opioids for chronic pain (http://stacks.cdc.gov/view/cdc/38025) as well as a website (http://www.cdc.gov/drugoverdose/prescribingresources.html) with additional tools to guide clinicians in implementing the recommendations. PMID:26987082

  19. Functional roles of PC-PLC and Cdc20 in the cell cycle, proliferation, and apoptosis.

    Science.gov (United States)

    Chen, Zhiwei; Yu, Yongfeng; Fu, Da; Li, Ziming; Niu, Xiaoming; Liao, Meilin; Lu, Shun

    2010-06-01

    Phosphatidylcholine-specific phospholipase C (PC-PLC) is the major enzyme in the Phosphatidylcholine (PC) cycle and is involved in many long-term cellular responses such as activation, proliferation, and differentiation events. Cell division cycle 20 homolog (Cdc20) is an essential cell-cycle regulator required for the completion of mitosis. Our previous studies identified the interaction between PC-PLC and Cdc20. Through the interaction, Cdc20 could mediate the degradation of PC-PLC by Cdc20-mediated ubiquitin proteasome pathway (UPP). In this study, we found that PC-PLC might not be involved in cancer metastasis. Inhibition of PC-PLC by D609 could cause cell proliferation inhibition and apoptosis inhibition in CBRH-7919 cells. Inhibition of PC-PLC could also influence the cell cycle by arresting the cells in G1 phase, and Cdc20 might be involved in these processes. Taken together, in this report, we provided new evidence for the functional roles of PC-PLC and Cdc20 in the cell cycle, proliferation, and apoptosis in CBRH-7919 cells.

  20. Human TRIB2 Oscillates during the Cell Cycle and Promotes Ubiquitination and Degradation of CDC25C

    Science.gov (United States)

    Liang, Kai Ling; Paredes, Roberto; Carmody, Ruaidhri; Eyers, Patrick A.; Meyer, Stefan; McCarthy, Tommie V.; Keeshan, Karen

    2016-01-01

    Tribbles homolog 2 (TRIB2) is a member of the mammalian Tribbles family of serine/threonine pseudokinases (TRIB1-3). Studies of TRIB2 indicate that many of the molecular interactions between the single Drosophila Tribbles (Trbl) protein and interacting partners are evolutionary conserved. In this study, we examined the relationship between TRIB2 and cell division cycle 25 (CDC25) family of dual-specificity protein phosphatases (mammalian homologues of Drosophila String), which are key physiological cell cycle regulators. Using co-immunoprecipitation we demonstrate that TRIB2 interacts with CDC25B and CDC25C selectively. Forced overexpression of TRIB2 caused a marked decrease in total CDC25C protein levels. Following inhibition of the proteasome, CDC25C was stabilized in the nuclear compartment. This implicates TRIB2 as a regulator of nuclear CDC25C turnover. In complementary ubiquitination assays, we show that TRIB2-mediated degradation of CDC25C is associated with lysine-48-linked CDC25C polyubiquitination driven by the TRIB2 kinase-like domain. A cell cycle associated role for TRIB2 is further supported by the cell cycle regulated expression of TRIB2 protein levels. Our findings reveal mitotic CDC25C as a new target of TRIB2 that is degraded via the ubiquitin proteasome system. Inappropriate CDC25C regulation could mechanistically underlie TRIB2 mediated regulation of cellular proliferation in neoplastic cells. PMID:27563873

  1. Cellular localization and biochemical analysis of mammalian CDC50A, a glycosylated β-subunit for P4 ATPases.

    Science.gov (United States)

    Folmer, Dineke E; Mok, Kam S; de Wee, Sebastiaan W; Duijst, Suzanne; Hiralall, Johan K; Seppen, Jurgen; Oude Elferink, Ronald P J; Paulusma, Coen C

    2012-03-01

    CDC50 proteins are β-subunits for P4 ATPases, which upon heterodimerization form a functional phospholipid translocation complex. Emerging evidence in mouse models and men links mutations in P4 ATPase genes with human disease. This study analyzed the tissue distribution and cellular localization of CDC50A, the most abundant and ubiquitously expressed CDC50 homologue in the mouse. The authors have raised antibodies that detect mouse and human CDC50A and studied CDC50A localization and glycosylation status in mouse liver cells. CDC50A is a terminal-glycosylated glycoprotein and is expressed in hepatocytes and liver sinusoidal endothelial cells, where it resides in detergent-resistant membranes. In pancreas and stomach, CDC50A localized to secretory vesicles, whereas in the kidney, CDC50A localized to the apical region of proximal convoluted tubules of the cortex. In WIF-B9 cells, CDC50A partially costains with the trans-Golgi network. Data suggest that CDC50A is present as a fully glycosylated protein in vivo, which presumes interaction with distinct P4 ATPases. PMID:22253360

  2. Human TRIB2 Oscillates during the Cell Cycle and Promotes Ubiquitination and Degradation of CDC25C.

    Science.gov (United States)

    Liang, Kai Ling; Paredes, Roberto; Carmody, Ruaidhri; Eyers, Patrick A; Meyer, Stefan; McCarthy, Tommie V; Keeshan, Karen

    2016-01-01

    Tribbles homolog 2 (TRIB2) is a member of the mammalian Tribbles family of serine/threonine pseudokinases (TRIB1-3). Studies of TRIB2 indicate that many of the molecular interactions between the single Drosophila Tribbles (Trbl) protein and interacting partners are evolutionary conserved. In this study, we examined the relationship between TRIB2 and cell division cycle 25 (CDC25) family of dual-specificity protein phosphatases (mammalian homologues of Drosophila String), which are key physiological cell cycle regulators. Using co-immunoprecipitation we demonstrate that TRIB2 interacts with CDC25B and CDC25C selectively. Forced overexpression of TRIB2 caused a marked decrease in total CDC25C protein levels. Following inhibition of the proteasome, CDC25C was stabilized in the nuclear compartment. This implicates TRIB2 as a regulator of nuclear CDC25C turnover. In complementary ubiquitination assays, we show that TRIB2-mediated degradation of CDC25C is associated with lysine-48-linked CDC25C polyubiquitination driven by the TRIB2 kinase-like domain. A cell cycle associated role for TRIB2 is further supported by the cell cycle regulated expression of TRIB2 protein levels. Our findings reveal mitotic CDC25C as a new target of TRIB2 that is degraded via the ubiquitin proteasome system. Inappropriate CDC25C regulation could mechanistically underlie TRIB2 mediated regulation of cellular proliferation in neoplastic cells. PMID:27563873

  3. Cdc6 localizes to S- and G2-phase centrosomes in a cell cycle-dependent manner

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Gwang Su; Kang, Jeeheon; Bang, Sung Woong; Hwang, Deog Su, E-mail: dshwang@snu.ac.kr

    2015-01-16

    Highlights: • Cdc6 protein is a component of the pre-replicative complex required for chromosomal replication initiation. • Cdc6 localized to centrosomes of S and G2 phases in a cell cycle-dependent manner. • The centrosomal localization was governed by centrosomal localization signal sequences of Cdc6. • Deletions or substitution mutations on the centrosomal localization signal interfered with centrosomal localization of the Cdc6 proteins. - Abstract: The Cdc6 protein has been primarily investigated as a component of the pre-replicative complex for the initiation of chromosome replication, which contributes to maintenance of chromosomal integrity. Here, we show that Cdc6 localized to the centrosomes during S and G2 phases of the cell cycle. The centrosomal localization was mediated by Cdc6 amino acid residues 311–366, which are conserved within other Cdc6 homologues and contains a putative nuclear export signal. Deletions or substitutions of the amino acid residues did not allow the proteins to localize to centrosomes. In contrast, DsRed tag fused to the amino acid residues localized to centrosomes. These results indicated that a centrosome localization signal is contained within amino acid residues 311–366. The cell cycle-dependent centrosomal localization of Cdc6 in S and G2 phases suggest a novel function of Cdc6 in centrosomes.

  4. The small GTPase Cdc42 modulates the number of exocytosis-competent dense-core vesicles in PC12 cells

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Mai [Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902 (Japan); Kitaguchi, Tetsuya [Cell Signaling Group, Waseda Bioscience Research Institute in Singapore (WABOIS), Waseda University, 11 Biopolis Way, 05-01/02 Helios, Singapore 138667 (Singapore); Numano, Rika [The Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka, Tennpaku-cho, Toyohashi, Aichi 441-8580 (Japan); Ikematsu, Kazuya [Forensic Pathology and Science, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523 (Japan); Kakeyama, Masaki [Laboratory of Environmental Health Sciences, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan); Murata, Masayuki; Sato, Ken [Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902 (Japan); Tsuboi, Takashi, E-mail: takatsuboi@bio.c.u-tokyo.ac.jp [Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902 (Japan)

    2012-04-06

    Highlights: Black-Right-Pointing-Pointer Regulation of exocytosis by Rho GTPase Cdc42. Black-Right-Pointing-Pointer Cdc42 increases the number of fusion events from newly recruited vesicles. Black-Right-Pointing-Pointer Cdc42 increases the number of exocytosis-competent dense-core vesicles. -- Abstract: Although the small GTPase Rho family Cdc42 has been shown to facilitate exocytosis through increasing the amount of hormones released, the precise mechanisms regulating the quantity of hormones released on exocytosis are not well understood. Here we show by live cell imaging analysis under TIRF microscope and immunocytochemical analysis under confocal microscope that Cdc42 modulated the number of fusion events and the number of dense-core vesicles produced in the cells. Overexpression of a wild-type or constitutively-active form of Cdc42 strongly facilitated high-KCl-induced exocytosis from the newly recruited plasma membrane vesicles in PC12 cells. By contrast, a dominant-negative form of Cdc42 inhibited exocytosis from both the newly recruited and previously docked plasma membrane vesicles. The number of intracellular dense-core vesicles was increased by the overexpression of both a wild-type and constitutively-active form of Cdc42. Consistently, activation of Cdc42 by overexpression of Tuba, a Golgi-associated guanine nucleotide exchange factor for Cdc42 increased the number of intracellular dense-core vesicles, whereas inhibition of Cdc42 by overexpression of the Cdc42/Rac interactive binding domain of neuronal Wiskott-Aldrich syndrome protein decreased the number of them. These findings suggest that Cdc42 facilitates exocytosis by modulating both the number of exocytosis-competent dense-core vesicles and the production of dense-core vesicles in PC12 cells.

  5. Distrito federal electoral No.34 del estado de California: un estudio de caso sobre la inclusión y representación de ciudadanos americanos de origen mexicano en el sistema político estadounidense

    OpenAIRE

    Cebreros Félix, Erika Eloisa

    2004-01-01

    A partir del censo poblacional de Estados Unidos de América de 2000, se determinó que los hispanos conformarían el grupo minoritario más grande del país, por lo que se les empezó a visualizar en términos políticos y específicamente electorales. Sin embargo, la historia y evolución de la inclusión y representación de los diferentes subgrupos que conforman dicha minoría ha sido poco desarrollada. El concepto de representación es uno de los aspectos fundamentales en el estud...

  6. Analysis of the influence of the expansion of the South American electric system in emissions of greenhouse gases; Analise da influencia da expansao do sistema eletrico Sul-Americano nas emissoes de gases de efeito estufa

    Energy Technology Data Exchange (ETDEWEB)

    Castagna, Annemarlen Gehrke [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Blesl, Markus [Institute of Economics and the Rational Use of Energie (IER), Stuttgart (Germany)

    2010-07-01

    South America combines economic and population growth with a consequent rapid increase in electricity demand. This can only be covered by building new power plants, use of the remaining renewable potential and expansion of transmission lines. The expansion of supply in all regions, with reliable generation and transmission systems is the greatest challenge for the continent in order to reduce social differences and not to curb economic development. To support the energy planning the application of system models represents useful method. This paper intends to analyze the expansion effect of power plant parks in regard of greenhouse gases emissions using a regionalized model system 'TIMES (The Integrated Markal - EFOM System)'. The model includes 10 South American countries (Argentina, Brazil, Bolivia, Chile, Colombia, Ecuador, Paraguay, Peru, Uruguay and Venezuela) with their respective power parks and transmission lines, demand divided in sectors, potential use of renewable energy sources, gas pipelines and possibilities of new interconnections within and between countries. As results are obtained the future installed capacity and generation according the energy use, greenhouse gases emissions, as well as the investments needed to expand the electric system in different scenarios. (author)

  7. Fortalecimiento del sistema judicial del Uruguay por la capacitación del sector humano; de la organización e integración de las escuelas judiciales americanos

    Directory of Open Access Journals (Sweden)

    Mariella Leles Da Silva Orcoyen

    2015-10-01

    Full Text Available Contenido: Introducción. Poder judicial. Ministerio Público y Fiscal. Estatuto de los jueces. Reforma procesal.  El fortalecimiento de la justicia por la reforma procesal. Centro de Estudios Judiciales del Uruguay. Capacitación.Capacitación para el ingreso a la magistratura. Resultados obtenidos. Estadísticas. Perspectivas futuras. Rol del Centro de Estudios Judiciales del Uruguay en la carrera judicial. Autoridades del Centro de Estudios Judiciales del Uruguay. Federación Americana de Escuelas de Formación y Perfeccionamiento de Magistrados y Centros de Estudios Judiciales

  8. Therapeutic Targeting the Cell Division Cycle 25 (CDC25 Phosphatases in Human Acute Myeloid Leukemia — The Possibility to Target Several Kinases through Inhibition of the Various CDC25 Isoforms

    Directory of Open Access Journals (Sweden)

    Annette K. Brenner

    2014-11-01

    Full Text Available The cell division cycle 25 (CDC25 phosphatases include CDC25A, CDC25B and CDC25C. These three molecules are important regulators of several steps in the cell cycle, including the activation of various cyclin-dependent kinases (CDKs. CDC25s seem to have a role in the development of several human malignancies, including acute myeloid leukemia (AML; and CDC25 inhibition is therefore considered as a possible anticancer strategy. Firstly, upregulation of CDC25A can enhance cell proliferation and the expression seems to be controlled through PI3K-Akt-mTOR signaling, a pathway possibly mediating chemoresistance in human AML. Loss of CDC25A is also important for the cell cycle arrest caused by differentiation induction of malignant hematopoietic cells. Secondly, high CDC25B expression is associated with resistance against the antiproliferative effect of PI3K-Akt-mTOR inhibitors in primary human AML cells, and inhibition of this isoform seems to reduce AML cell line proliferation through effects on NFκB and p300. Finally, CDC25C seems important for the phenotype of AML cells at least for a subset of patients. Many of the identified CDC25 inhibitors show cross-reactivity among the three CDC25 isoforms. Thus, by using such cross-reactive inhibitors it may become possible to inhibit several molecular events in the regulation of cell cycle progression and even cytoplasmic signaling, including activation of several CDKs, through the use of a single drug. Such combined strategies will probably be an advantage in human cancer treatment.

  9. El envío de indios americanos a la Península Ibérica: aspectos legales (1492-1542

    Directory of Open Access Journals (Sweden)

    Esteban MIRA CABALLOS

    2009-12-01

    Full Text Available RESUMEN: En este presente artículo ofrecemos por primera vez una sistematización sobre la legislación existente en torno a la trata de indios americanos con destino a los mercados esclavistas de la Península Ibérica. Se trata sin duda de un trabajo sumamente novedoso que aporta nueva luz a un aspecto de la Historia Moderna de España que ha pasado casi desapercibido hasta la fecha para la historiografía más reciente. La conclusión principal que se puede extraer de este trabajo es la negativa de la Corona -salvo en los dubitativos momentos iniciales- a que se trajesen indios esclavos a la Península. No obstante, esta legislación prohibitiva sólo sirvió para que los envíos se ralentizaran sin erradicar totalmente una trata que siempre encontró las formas de continuar de una manera más o menos fraudulenta.ABSTRACT: For the first time the present article offers us a systematization about the existing laws concerning the American indians trade with the Iberian Peninsula slaves markets. Undoubtledly, it consists of an extremely original work which casts light on one aspect of the Spanish early modern history that has gone unnoticed to the recent historiography. The main conclusion to be drawn from this work is the Crown's denial for the Indian slaves to be brought, except for the doubts arisen at the beginning. This prohibitive laws only served to slow down the slave shipment without eradicate this practice that always founds to continue new forms, more or less fraudulent.

  10. Atividade anti-helmíntica de plantas nativas do continente americano: uma revisão Anthelmintic activity of native American plants: a review

    Directory of Open Access Journals (Sweden)

    R.G. Sousa

    2013-01-01

    Full Text Available O presente trabalho faz uma revisão de plantas nativas do continente americano com atividade anti-helmíntica com a finalidade de subsidiar pesquisas e o desenvolvimento de novos medicamentos na área de produtos naturais. Na literatura, foram citadas 22 famílias de plantas, tais como: Moraceae, Asteraceae e Cucurbitaceae, e estudadas cerca de 35 espécies, como a Ficus sp, Cucurbita máxima, e Ruta graveolens, usadas por sua alegada atividade anti-helmíntica. Destas espécies, 26 foram ativas contra alguns helmintos: Strongyloides stercolaris, Ancylostomidae, Trichostrongylus sp, Capillaria sp, Ascaris lumbricoides, Taenia saginata, Haemonchus contortus, e Hymenolepsis diminuta. Foi verificado maior número de estudos com parasitas de animais, possivelmente devido ao desenvolvimento de resistência dos helmintos aos tratamentos convencionais disponíveis.This paper is a review of native American plants showing anthelmintic activity in order to give support to research and development of new drugs in the field of natural products. In the literature, 22 plant families were cited, such as: Moraceae, Asteraceae and Cucurbitaceae, and around 35 species were studied, including Ficus sp, Cucurbita maxima and Ruta graveolens, used for their putative anthelmintic activity. From these species, 26 were active against some helminthes: Strongyloides stercolaris, Ancylostomidae, Trichostrongylus sp, Capillaria sp, Ascaris lumbricoides, Taenia saginata, Haemonchus contortus and Hymenolepsis diminuta. Data indicated a larger number of studies with animal parasites, probably due to the resistance of these helminthes to the conventional treatments available.

  11. Depois das reformas: os regimes de proteção social latino-americanos na década de 2000

    Directory of Open Access Journals (Sweden)

    Rodrigo Cantu

    2015-12-01

    Full Text Available Resumo O artigo caracteriza os regimes de política social na América Latina durante a década de 2000, um contexto que vem sendo designado por uma penetração menos acentuada de políticas neoliberais. Usamos o método de Análise de Componentes Principais (ACP para analisar dados sobre o papel do Estado, do mercado e das famílias na proteção contra riscos sociais, recolhidos em fontes como CEPAL, FMI, Banco Mundial, OMS, entre outras. Comparando os dados para as décadas de 1990 e de 2000, identificamos certa estabilização das reformas neoliberais. Comparando ainda os resultados da ACP com a literatura, sobretudo com a literatura que se ocupa das décadas de 1980 e 1990, argumentamos que, mesmo atenuadas, as reformas deixaram o cenário da política social mais complexo na América Latina na década de 2000. Uma primeira dimensão, já amplamente discutida na literatura, foi encontrada na ACP, definida pelo grau em que o Estado inclui a população em suas políticas sociais. Ademais, uma segunda dimensão relevante foi revelada pela análise, captando em que profundidade o mercado se instalou como princípio da política social. Discutimos a hipótese segundo a qual essa dimensão se tornou expressiva após as reformas das décadas de 1980 e 1990. Junto com o argumento de que a literatura precisa ser atualizada com base nesse quadro mais complexo, destacamos a diversidade dos regimes de política social latino-americanos tanto internamente quanto em comparação com outras regiões do mundo.

  12. Las maestras y el ensayo como escritura cívica femenina en Repertorio Americano / Women Teachers, Essays and Civic Writing in “Repertorio Americano”

    Directory of Open Access Journals (Sweden)

    Marybel Soto Ramírez

    2011-11-01

    Full Text Available Recibido 17 de mayo de 2011 • Aceptado 26 de agosto de 2011 • Corregido 9 de octubre de 2011 Resumen. La escritura femenina, como todas las formas de producción simbólica desde las mujeres, ha existido y se ha desarrollado a pesar del mandato patriarcal de obedecer y callar que pesa sobre el género femenino. No es objetivo en esta reflexión, ahondar en las acciones y las obras de las mujeres transgresoras quienes, a lo largo de la historia y a pesar de, han ejercido esa forma de hacerse presente y afirmarse ante los otros que es la escritura. Mi propósito es identificar a las mujeres, quienes desde el oficio de maestras ejercitaron su escritura, combinaron su rol docente con el de escritoras y en la elaboración de sus discursos cívicos cuestionaron, precisamente, las concepciones de patriotismo y sentimiento cívico dentro del mismo proceso de construcción de una ciudadanía de la cual ellas no disfrutaban. Abstract. Women writing, as any symbolic form of production, has existed and developed despite of the patriarchal imperative commanding women to obey and to remain silent. It is not the objective of this essay to deepen on the deeds and doings of those women who, throughout history and despite patriarchal mandates, have exerted their writing as a transgression as a way to show and to affirm themselves before others. Moreover, my purpose is to identify women in Repertorio Americano (1919-1958 who as teachers and writers elaborated civic discourses and questioned the very meaning of patriotism and civic feelings in the constructions of a citizenship they were not fully and formally entitled to.

  13. Sistema Distribuido de Gran Escala

    Directory of Open Access Journals (Sweden)

    Fco. Javier Luna Rosas

    2006-01-01

    Full Text Available Este artículo propone una nueva forma de balancear la carga dinámicamente combinando un modelo Fuzzy-Logic en conjunto con el algoritmo distribuido Request for Bids (RB, el trabajo es centrado en el modelo Fuzzy-Logic con el propósito de mejorar el balanceo dinámico de carga en un sistema distribuido a gran escala. El modelo Fuzzy-Logic y RB fueron implementados en una arquitectura para balancear la carga bajo el estándar de CORBA y fueron evaluados contra otras estrategias que son bien conocidas en la literatura usando diferentes métricas de carga y mostramos como bajo ciertas condiciones nuestra estrategia obtiene mejores resultados.

  14. Hepatocyte-specific deletion of Cdc42 results in delayed liver regeneration after partial hepatectomy in mice

    DEFF Research Database (Denmark)

    Yuan, Haixin; Zhang, Hong; Wu, Xunwei;

    2009-01-01

    Cdc42, a member of the Rho guanosine triphosphatase (GTPase) family, plays important roles in the regulation of the cytoskeleton, cell proliferation, cell polarity, and cellular transport, but little is known about its specific function in mammalian liver. We investigated the function of Cdc42 in...... regeneration, which may result from impaired cytoskeletal organization and intracellular trafficking in hepatocytes. Conclusion: Our results revealed important roles of Cdc42 in the regulation of proliferative signaling during liver regeneration....

  15. Sistemas de comunicaciones para vuelos no tripulados

    OpenAIRE

    Correa Vila, Alejandro

    2008-01-01

    La iniciativa ATLANTIDA engloba numerosos estudios de ingeniería con el objetivo de reformar el actual sistema de gestión del tráfico aéreo. Dentro de estos estudios se encuentran diversas investigaciones en el campo de las comunicaciones móviles. La gran variabilidad del entorno aeronáutico promueve el estudio de sistemas de comunicaciones móviles capaces de funcionar en este tipo de entorno. Este proyecto propone un sistema de comunicaciones WiMAX en un entorno aeronáutico con aviones no tr...

  16. Sistemas Avanzados de Asistencia al Conductor

    OpenAIRE

    Jimenez Ruiz, Francisco; Gómez Casado, Oscar; Naranjo Hernández, José Eugenio; Serradilla García, Francisco; López, José María; Martín, Ángel

    2011-01-01

    El control inteligente de vehículos autónomos es uno de los retos actuales más importantes de los Sistemas Inteligentes de Transporte. La aplicación de técnicas de inteligencia artificial para la gestión automática de los actuadores del vehículo permite a los diferentes sistemas avanzados de asistencia al conductor (ADAS) y a los sistemas de conducción autónoma, realizar una gestión de nivel bajo de una manera muy similar a la de los conductores humanos, mejorando la seguridad y el confor...

  17. Sistemas basados en conocimiento usando Prolog

    OpenAIRE

    Zenaida García Valdivia; Isis Bonet Cruz; Maikel León Espinosa; Pedro Y. Piñero Pérez

    2011-01-01

    Se exponen algunos de los resultados alcanzados en la Universidad Central "Marta Abreu" de Las Villas en cuanto al uso de un lenguaje de Programación Lógica (Prolog) en la elaboración de Sistemas Basados en Conocimiento para diferentes áreas del saber como son la medicina, soldadura, educación, sistemas eléctricos de potencia, entre otros. Todos los sistemas elaborados utilizan el paradigma de la programación lógica para la representación del conocimiento de los expertos en los temas en cuest...

  18. Sistemas agroforestales ¿pasado o futuro?

    OpenAIRE

    Arribas Lumbreras, Teresa; Blas Morente, Alejandro; Barreiro Elorza, Pilar; Hernández Díaz-Ambrona, Carlos Gregorio

    2011-01-01

    Los sistemas agroforestales han sufrido un decrecimiento continuo durante las últimas décadas, en especial en países de la UE debido a la PAC. La estimación para el año 2050 según la FAO indica la necesidad de aumentar considerablemente la cantidad de alimentos, y por tanto dichos sistemas mixtos árbol-cultivo pueden convertirse en técnicas efectivas y rentables de cultivo. Mediante la exposición de dos casos prácticos, en Colombia y España, se describirá cómo los sistemas agroforestales pued...

  19. Soporte de sistemas para procesos colaborativos

    OpenAIRE

    Grosclaude, Eduardo

    2005-01-01

    Las experiencias de enseñanza-aprendizaje basadas en plataformas colaborativas se ven afectadas por problemas de disponibilidad provocados por diversos modos de falla de los sistemas que los soportan. El concepto de disponibilidad sugiere incorporar a la gestión de los sistemas la prevención y resolución de esos eventos de falla. La identi cación y eliminación de los puntos únicos de falla mediante el patrón básico de la redundancia permite aproximar el comportamiento de los sistemas resilien...

  20. Share!: Sistema para compartir contenidos multimedia

    OpenAIRE

    Cimas Bravo, Eduardo

    2015-01-01

    Este trabajo consiste en un sistema informático destinado a la compartición de contenidos multimedia entre grupos restringidos de usuarios. Los contenidos pueden ser imágenes, vídeos y audios. Este sistema se compone de dos aplicaciones, una aplicación web y una aplicación móvil para dispositivos Android. La esencia del sistema es proporcionar un mecanismo para compartir rápidamente contenidos multimedia entre usuarios. Los contenidos pueden estar ya en sus dispositivos móviles o ser capturad...

  1. The F-box protein Cdc4/Fbxw7 is a novel regulator of neural crest development in Xenopus laevis

    Directory of Open Access Journals (Sweden)

    Hartley Rebecca S

    2010-01-01

    Full Text Available Abstract Background The neural crest is a unique population of cells that arise in the vertebrate ectoderm at the neural plate border after which they migrate extensively throughout the embryo, giving rise to a wide range of derivatives. A number of proteins involved in neural crest development have dynamic expression patterns, and it is becoming clear that ubiquitin-mediated protein degradation is partly responsible for this. Results Here we demonstrate a novel role for the F-box protein Cdc4/Fbxw7 in neural crest development. Two isoforms of Xenopus laevis Cdc4 were identified, and designated xCdc4α and xCdc4β. These are highly conserved with vertebrate Cdc4 orthologs, and the Xenopus proteins are functionally equivalent in terms of their ability to degrade Cyclin E, an established vertebrate Cdc4 target. Blocking xCdc4 function specifically inhibited neural crest development at an early stage, prior to expression of c-Myc, Snail2 and Snail. Conclusions We demonstrate that Cdc4, an ubiquitin E3 ligase subunit previously identified as targeting primarily cell cycle regulators for proteolysis, has additional roles in control of formation of the neural crest. Hence, we identify Cdc4 as a protein with separable but complementary functions in control of cell proliferation and differentiation.

  2. Redundant and nonredundant roles for Cdc42 and Rac1 in lymphomas developed in NPM-ALK transgenic mice.

    Science.gov (United States)

    Choudhari, Ramesh; Minero, Valerio Giacomo; Menotti, Matteo; Pulito, Roberta; Brakebusch, Cord; Compagno, Mara; Voena, Claudia; Ambrogio, Chiara; Chiarle, Roberto

    2016-03-10

    Increasing evidence suggests that Rho family GTPases could have a critical role in the biology of T-cell lymphoma. In ALK-rearranged anaplastic large cell lymphoma (ALCL), a specific subtype of T-cell lymphoma, the Rho family GTPases Cdc42 and Rac1 are activated by the ALK oncogenic activity. In vitro studies have shown that Cdc42 and Rac1 control rather similar phenotypes of ALCL biology such as the proliferation, survival, and migration of lymphoma cells. However, their role and possible redundancy in ALK-driven lymphoma development in vivo are still undetermined. We genetically deleted Cdc42 or Rac1 in a mouse model of ALK-rearranged ALCL to show that either Cdc42 or Rac1 deletion impaired lymphoma development, modified lymphoma morphology, actin filament distribution, and migration properties of lymphoma cells. Cdc42 or Rac1 deletion primarily affected survival rather than proliferation of lymphoma cells. Apoptosis of lymphoma cells was equally induced following Cdc42 or Rac1 deletion, was associated with upregulation of the proapoptotic molecule Bid, and was blocked by Bcl2 overexpression. Remarkably, Cdc42/Rac1 double deletion, but not Cdc42 or Rac1 single deletions, completely prevented NPM-ALK lymphoma dissemination in vivo. Thus, Cdc42 and Rac1 have nonredundant roles in controlling ALK-rearranged lymphoma survival and morphology but are redundant for lymphoma dissemination, suggesting that targeting both GTPases could represent a preferable therapeutic option for ALCL treatment.

  3. Anti-CDC25B autoantibody predicts poor prognosis in patients with advanced esophageal squamous cell carcinoma

    OpenAIRE

    Dong Jun; Zeng Bo-hang; Xu Li-hua; Wang Jun-ye; Li Man-Zhi; Zeng Mu-sheng; Liu Wan-li

    2010-01-01

    Abstract Background The oncogene CDC25B phosphatase plays an important role in cancer cell growth. We have recently reported that patients with esophageal squamous cell carcinoma (ESCC) have significantly higher serum levels of CDC25B autoantibodies (CDC25B-Abs) than both healthy individuals and patients with other types of cancer; however, the potential diagnostic or prognostic significance of CDC25B-Abs is not clear. The aim of this study is to evaluate the clinical significance of serum CD...

  4. Staphylococcus aureus recruits Cdc42GAP through recycling endosomes and the exocyst to invade human endothelial cells.

    Science.gov (United States)

    Rauch, Liane; Hennings, Kirsten; Trasak, Claudia; Röder, Anja; Schröder, Barbara; Koch-Nolte, Friedrich; Rivera-Molina, Felix; Toomre, Derek; Aepfelbacher, Martin

    2016-08-01

    Activation and invasion of the vascular endothelium by Staphylococcus aureus is a major cause of sepsis and endocarditis. For endothelial cell invasion, S. aureus triggers actin polymerization through Cdc42, N-WASp (also known as WASL) and the Arp2/3 complex to assemble a phagocytic cup-like structure. Here, we show that after stimulating actin polymerization staphylococci recruit Cdc42GAP (also known as ARHGAP1) which deactivates Cdc42 and terminates actin polymerization in the phagocytic cups. Cdc42GAP is delivered to the invading bacteria on recycling endocytic vesicles in concert with the exocyst complex. When Cdc42GAP recruitment by staphylococci was prevented by blocking recycling endocytic vesicles or the exocyst complex, or when Cdc42 was constitutively activated, phagocytic cup closure was impaired and endothelial cell invasion was inhibited. Thus, to complete invasion of the endothelium, staphylococci reorient recycling endocytic vesicles to recruit Cdc42GAP, which terminates Cdc42-induced actin polymerization in phagocytic cups. Analogous mechanisms might govern other Cdc42-dependent cell functions.

  5. Drug design with Cdc7 kinase: a potential novel cancer therapy target

    Directory of Open Access Journals (Sweden)

    Masaaki Sawa

    2008-11-01

    Full Text Available Masaaki Sawa1, Hisao Masai21Carna Biosciences, Inc., Kobe, Japan; 2Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanAbstract: Identification of novel molecular targets is critical in development of new and efficient cancer therapies. Kinases are one of the most common drug targets with a potential for cancer therapy. Cell cycle progression is regulated by a number of kinases, some of which are being developed to treat cancer. Cdc7 is a serine-threonine kinase originally discovered in budding yeast, which has been shown to be necessary to initiate the S phase. Inhibition of Cdc7 in cancer cells retards the progression of the S phase, accumulates DNA damage, and induces p53-independent cell death, but the same treatment in normal cells does not significantly affect viability. Low-molecular-weight compounds that inhibit Cdc7 kinase with an IC50 of less than 10 nM have been identified, and shown to be effective in the inhibition of tumor growth in animal models. Thus Cdc7 kinase can be recognized as a novel molecular target for cancer therapy.Keywords: Cdc7 kinase, cell cycle, replication fork, genome stability, DNA damages, ATP-binding pocket, kinase inhibitor

  6. Temperature dependent expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    p34cdc2 and Cyclin Bi are key components of cell cycle controlling machine and are believed to play a fundamental role in gametogenesis. It is also well known that, in scrotal mammals, spermatogenesis depends greatly on the maintenance of comparatively low temperature in the scrotum. To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that the abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes, but it blocked the translation of them. Due to the deficiency of p34cdc2 and Cyclin B1, the spermatogonia and pachytene/diplotene primary spermatocytes were unable to form MPF, hence, they couldn't undergo karyokinesis. The development of primary spermato cytes was arrested at the G2 to M phase transition. We also found that testosterone could regulate the Cyclin B1 expression in spermatogenic cells. Muscular injection of testosterone could recover spermatogenesis in the unilateral scrotal testis which was influenced by the contralateral cryptorchid testis, but it could not salvage the spermatogenesis block in the cryptorchid testis.

  7. JUAN M. LOPE BLANCH. El habla de Diego de Ordaz. Contribución a la historia del español americano. México, UNAM. 1985. 233 p.

    Directory of Open Access Journals (Sweden)

    J. L. R.

    1988-06-01

    Full Text Available Este libro reúne los trabajos publicados por Lope Blanch en revistas especializadas y homenajes académicos acerca del idiolecto de este importante americano nacido en tierras leonesas hacia 1480, colonizador de las Antillas, expedicionario a Tierra Firme y a Cuba, conquistador de México y explorador del Orinoco. La base documental está constituida por siete cartas autógrafasque L.B. publica al final del libro en una cuidadosa transcripción que corrige defectos de una edición anterior.

  8. Una actividad de educación ambiental basada en observaciones del cangrejo de río americano (Procambarus clarkii) en el Barranco del Cercado (Tenerife, Islas Canarias)

    OpenAIRE

    Mingorance Rodríguez, María del Carmen; Gómez Gómez, Juan Imeldo

    2002-01-01

    El Procambarus clarkii (cangrejo de río americano o cangrejo rojo de las marismas) es un ejemplo de la introducción de una especie en un ecosistema. En este trabajo se utiliza una población de esta especie en la isla de Tenerife para un conjunto de actividades educativas en un Instituto de Formación Profesional. Estas actividades incluyen muestreos y capturas de los cangrejos en el campo, la toma de sus medidas iniciales y su mantenimiento en el Instituto.

  9. Sistemas basados en conocimiento usando Prolog

    Directory of Open Access Journals (Sweden)

    Zenaida García Valdivia

    2011-10-01

    Full Text Available Se exponen algunos de los resultados alcanzados en la Universidad Central "Marta Abreu" de Las Villas en cuanto al uso de un lenguaje de Programación Lógica (Prolog en la elaboración de Sistemas Basados en Conocimiento para diferentes áreas del saber como son la medicina, soldadura, educación, sistemas eléctricos de potencia, entre otros. Todos los sistemas elaborados utilizan el paradigma de la programación lógica para la representación del conocimiento de los expertos en los temas en cuestión y además hacen uso de entornos agradables a los usuarios a través interfaces visuales elaboradas en ambientes de programación para súper lenguajes ó utilizan un conjunto de herramientas para la elaboración de sistemas sobre la Web

  10. El Sistema Nacional de Cualificaciones Profesionales

    OpenAIRE

    Brezmes Arceredillo, Ricardo

    2014-01-01

    El trabajo aborda el Sistema Nacional de Cualificaciones y Formación Profesional desgranando anteriormente conceptos básicos para su entendimiento como son las competencias laborales y profesionales, las cualificaciones profesionales y el Catálogo Nacional de Cualificaciones Profesionales entre otros. Se trata de analizar estos conceptos y reflexionar sobre ellos en las conclusiones de trabajo para así extraer una idea de lo que el Sistema Nacional de Cualificaciones y Formació...

  11. Isolation and Characterization of New Alleles of the Cyclin-Dependent Kinase Gene CDC28 with Cyclin-Specific Functional and Biochemical Defects

    OpenAIRE

    Levine, Kristi; Oehlen, L. J. W. M.; Cross, Frederick R.

    1998-01-01

    The G1 cyclin Cln2 negatively regulates the mating-factor pathway. In a genetic screen to identify factors required for this regulation, we identified an allele of CDC28 (cdc28-csr1) that blocked this function of Cln2. Cln2 immunoprecipitated from cdc28-csr1 cells was completely defective in histone H1 kinase activity, due to defects in Cdc28 binding and activation by Cln2. In contrast, Clb2-associated H1 kinase and Cdc28 binding was normal in immunoprecipitates from these cells. cdc28-csr1 w...

  12. Gerencia de sistemas conductuales y cambio organizacional

    Directory of Open Access Journals (Sweden)

    Guillermo E. Yáber

    1998-01-01

    Full Text Available La gerencia de sistemas conductuales es una tecnología basada en un enfoque científico para el análisis del comportamiento en sistemas humanos. Esta tecnología ayuda a las organizaciones a definir y lograr sus metas. Consiste en el análisis, especificación, diseño, implementación, evaluación y reciclaje de sistemas de motivación humana que optimizan el desempeño y desarrollo a nivel del sistema total, departamental e individual de la organización. Utiliza tres modelos: El diseño de sistemas dirigidos hacia la meta, el análisis de «Tres Contingencias» y la gerencia del desempeño para el cambio cultural. Este artículo incluye la descripción del marco teórico y metodológico de la gerencia de sistemas conductuales y su aplicación en las áreas de servicios y producción.

  13. 超表达Cdc20基因不影响牛卵母细胞第一极体排出%Over-expression of Cdc20 Gene Has No Effect on Bovine Oocytes First Polar Body Extrusion

    Institute of Scientific and Technical Information of China (English)

    杨文琳; 安鹏; 李伟; 赵贵民; 史芸安; 雷安民

    2012-01-01

    As one of the co-activator of anaphase-promoting complex ( APC) , cell division cycle 20 (CDC20) protein also functions as the target of the spindle assembly checkpoint ( SAC), which is essential for the cell cycle regulation. To investigate the function of Cdc20 during the first polar body extrusion ( PBE I) , Cdc20 CDS was cloned and eukaryotic expression vector pCdc20-Venus was constructed. Using the linear pCdc20-Venus as template, the capped Cdc20-Venus mRNA was synthesized via T7 Mmessage Mmachine Kit ( Ambion). Cdc20 over-expression was performed by microinjection of Cdc20-Venus mRNA into the cytoplasm of bovine oocytes. The results showed that Venus tagged Cdc20 dispersed around the nucleus in HeLa cells. In bovine oocytes, the fluorescence appeared in the whole cytoplasm. However, the PBE I rate in over-expressed group (48. 9% ) is not significant, compared to Venus mRNA injection group (50.9%) and non-injection group (51.1%). Our study demonstrated that the over-expression of Cdc20 in bovine oocytes does not affect the PBE I rate ( P > 0.05).%CDC20(cell division cycle 20)是后期促进复合物(anaphase-promoting complex,APC)的共激活剂之一,也是纺锤体组装检查点(spindle assembly checkpoint,SAC)的靶点,在细胞周期调控中扮演重要角色.为探讨Cdc20在第一极体排出(first polar body extrusion,PBE I)中的作用,Cdc20基因被成功克隆并构建了真核表达载体pCdc20-Venus,随后用T7 Mmessage Mmachine Kit(Ambion)以线性化pCdc20-Venus为模板体外转录(in vitro transcription)获得带帽的Cdc20-Venus mRNA,将Ccdc20-Venus mRNA显微注射到体外培养的牛卵母细胞胞质中进行超量表达.结果表明,真核表达载体pCdc20-Venus转染HeLa细胞后能够正常表达,绿色荧光在细胞核周围呈弥散状分布;将Cdc20-Venus mRNA注射到牛卵母细胞胞质后,胞质内有绿色荧光出现.Cdc20-Venus mRNA注射组卵母细胞的PBE I率(48.9%)与Venus mRNA注射组卵母细胞的PBE I率(50

  14. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1.

    Science.gov (United States)

    Lee, Seung Baek; Kim, Jung Jin; Nam, Hyun-Ja; Gao, Bowen; Yin, Ping; Qin, Bo; Yi, Sang-Yeop; Ham, Hyoungjun; Evans, Debra; Kim, Sun-Hyun; Zhang, Jun; Deng, Min; Liu, Tongzheng; Zhang, Haoxing; Billadeau, Daniel D; Wang, Liewei; Giaime, Emilie; Shen, Jie; Pang, Yuan-Ping; Jen, Jin; van Deursen, Jan M; Lou, Zhenkun

    2015-10-01

    Mutations in the E3 ubiquitin ligase Parkin have been linked to familial Parkinson's disease. Parkin has also been implicated in mitosis through mechanisms that are unclear. Here we show that Parkin interacts with anaphase promoting complex/cyclosome (APC/C) coactivators Cdc20 and Cdh1 to mediate the degradation of several key mitotic regulators independent of APC/C. We demonstrate that ordered progression through mitosis is orchestrated by two distinct E3 ligases through the shared use of Cdc20 and Cdh1. Furthermore, Parkin is phosphorylated and activated by polo-like kinase 1 (Plk1) during mitosis. Parkin deficiency results in overexpression of its substrates, mitotic defects, genomic instability, and tumorigenesis. These results suggest that the Parkin-Cdc20/Cdh1 complex is an important regulator of mitosis.

  15. Dynamics of Cdc42 network embodies a Turing-type mechanism of yeast cell polarity.

    Science.gov (United States)

    Goryachev, Andrew B; Pokhilko, Alexandra V

    2008-04-30

    Complex biochemical networks can be understood by identifying their principal regulatory motifs and mode of action. We model the early phase of budding yeast cellular polarization and show that the biochemical processes in the presumptive bud site comprise a Turing-type mechanism. The roles of the prototypical activator and substrate are played by GTPase Cdc42 in its active and inactive states, respectively. We demonstrate that the nucleotide cycling of Cdc42 converts cellular energy into a stable cluster of activated Cdc42. This energy drives a continuous membrane-cytoplasmic exchange of the cluster components to counteract diffusive spread of the cluster. This exchange explains why only one bud forms per cell cycle, because the winner-takes-all competition of candidate sites inevitably selects a single site. PMID:18381072

  16. Anti-CDC25B autoantibody predicts poor prognosis in patients with advanced esophageal squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Dong Jun

    2010-09-01

    Full Text Available Abstract Background The oncogene CDC25B phosphatase plays an important role in cancer cell growth. We have recently reported that patients with esophageal squamous cell carcinoma (ESCC have significantly higher serum levels of CDC25B autoantibodies (CDC25B-Abs than both healthy individuals and patients with other types of cancer; however, the potential diagnostic or prognostic significance of CDC25B-Abs is not clear. The aim of this study is to evaluate the clinical significance of serum CDC25B-Abs in patients with ESCC. Methods CDC25B autoantibodies were measured in sera from both 134 patients with primary ESCC and 134 healthy controls using a reverse capture enzyme-linked immunosorbent assay (ELISA in which anti-CDC25B antibodies bound CDC25B antigen purified from Eca-109 ESCC tumor cells. The clinicopathologic significance of CDC25B serum autoantibodies was compared to that of the tumor markers carcinoembryonic antigen (CEA, squamous cell carcinoma antigen (SCC-Ag and cytokeratin 19 fragment antigen 21-1(CYFRA21-1. Results Higher levels of CDC25B autoantibodies were present in sera from patients with ESCC (A450 = 0.917, SD = 0.473 than in sera from healthy control subjects (A450 = 0.378, SD = 0.262, P 450 greater than the cut-off value of 0.725. Relatively few patients tested positive for the tumor markers CEA, SCC-Ag and CYFRA21-1 (13.4%, 17.2%, and 32.1%, respectively. A significantly higher number of patients with ESCC tested positive for a combination of CEA, SCC, CYFRA21-1 and CDC25B-Abs (64.2% than for a combination of CEA, SCC-Ag and CYFRA21-1 (41.0%, P P P = 0.001, log-rank. In the N1 subgroup, the cumulative five-year survival rate of CDC25B-seropositive patients was 13.6%, while that of CDC25B-seronegative patients was 54.5% (P = 0.040, log-rank. Conclusions Detection of serum CDC25B-Abs is superior to detection of the tumor markers CEA, SCC-Ag and CYFRA21-1 for diagnosis of ESCC, and CDC25B-Abs are a potential prognostic

  17. Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Zoltán Kevei

    Full Text Available BACKGROUND: The CDC20 and Cdh1/CCS52 proteins are substrate determinants and activators of the Anaphase Promoting Complex/Cyclosome (APC/C E3 ubiquitin ligase and as such they control the mitotic cell cycle by targeting the degradation of various cell cycle regulators. In yeasts and animals the main CDC20 function is the destruction of securin and mitotic cyclins. Plants have multiple CDC20 gene copies whose functions have not been explored yet. In Arabidopsis thaliana there are five CDC20 isoforms and here we aimed at defining their contribution to cell cycle regulation, substrate selectivity and plant development. METHODOLOGY/PRINCIPAL FINDINGS: Studying the gene structure and phylogeny of plant CDC20s, the expression of the five AtCDC20 gene copies and their interactions with the APC/C subunit APC10, the CCS52 proteins, components of the mitotic checkpoint complex (MCC and mitotic cyclin substrates, conserved CDC20 functions could be assigned for AtCDC20.1 and AtCDC20.2. The other three intron-less genes were silent and specific for Arabidopsis. We show that AtCDC20.1 and AtCDC20.2 are components of the MCC and interact with mitotic cyclins with unexpected specificity. AtCDC20.1 and AtCDC20.2 are expressed in meristems, organ primordia and AtCDC20.1 also in pollen grains and developing seeds. Knocking down both genes simultaneously by RNAi resulted in severe delay in plant development and male sterility. In these lines, the meristem size was reduced while the cell size and ploidy levels were unaffected indicating that the lower cell number and likely slowdown of the cell cycle are the cause of reduced plant growth. CONCLUSIONS/SIGNIFICANCE: The intron-containing CDC20 gene copies provide conserved and redundant functions for cell cycle progression in plants and are required for meristem maintenance, plant growth and male gametophyte formation. The Arabidopsis-specific intron-less genes are possibly "retrogenes" and have hitherto undefined

  18. Cogeneration Systems; Sistemas de Cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez M, Manuel F; Huante P, Liborio; Romo M, Cesar A [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2006-07-01

    The present article deals on relevant aspects on the subject of cogeneration within the Mexican territorial limits. In the first place it is presented the role of Mexico in terms of its cogeneration potential, the type of service that has obtained from this predominant modality of cogeneration for self-supplying, the most propitious sectors to develop it, its legislations on the matter, the projects made for the implementation of cogeneration plants, as well as the existing cogeneration schemes for its respective optimization proposals. Without leaving out the analysis on the different types of evaluation on the efficiency of cogeneration systems and the aspects to consider for the election of a generation cycle. [Spanish] El presente articulo trata sobre aspectos relevantes en materia de cogeneracion dentro de los limites territoriales de la nacion mexicana. Se muestra en primer lugar el papel de Mexico en terminos de su potencial de cogeneracion, el tipo de servicio que ha obtenido de esta predominantemente (modalidad de cogeneracion para autoabastecimiento), los sectores mas propicios para desarrollarla, sus legislaciones al respecto, los proyectos realizados para la implementacion de plantas de cogeneracion, asi como los esquemas de cogeneracion existentes con sus respectivas propuestas de optimizacion. Sin dejar de lado el analisis sobre los distintos tipos de evaluacion de la eficiencia de sistemas de cogeneracion y los aspectos a considerar para la eleccion de un ciclo de generacion.

  19. Legislativo e política comercial: a aprovação do TLC com os Estados Unidos nos legislativos sul-americanos

    Directory of Open Access Journals (Sweden)

    Pedro Feliú Ribeiro

    2012-11-01

    Full Text Available O artigo analisa as votações nominais de três poderes legislativos sul-americanos, Chile, Colômbia e Peru, acerca da ratificação do Tratado de Livre Comércio com os Estados Unidos. A pergunta central é: quais são os fatores determinantes do voto do legislador na aprovação do Tratado de Livre Comércio (TLC com os Estados Unidos? Três fatores centrais emergem da literatura: (i as relações Estados Unidos x América Latina produzem uma clivagem ideológica (direita-esquerda entre os partidos políticos do continente; (ii o pertencimento do legislador à coalizão de governo no poder Legislativo e (iii os fatores sócio-econômicos dos distritos eleitorais dos legisladores. Por meio da utilização do modelo de regressão logística, apontamos que a ideologia dos legisladores peruanos explica os votos dos mesmos no TLC com os Estados Unidos, indicando que quanto mais à esquerda estiver o legislador, menor a probabilidade do mesmo aprovar o referido tratado. No caso chileno, a taxa de desemprego do distrito eleitoral do deputado possui maior capacidade explicativa, revelando que quanto maior a taxa de desemprego, menor a probabilidade de aprovação do TLC com os Estados Unidos. Já no caso do Senado colombiano, há uma forte associação entre o pertencimento do Senador à coalizão de governo e a aprovação do TLC, revelando a influência dessa variável institucional no voto do Senado colombiano. Além do pertencimento ou não à coalizão de governo, a ideologia do partido político do legislador colombiano também se apresenta como fator explicativo relevante do voto do mesmo.

  20. Experience in programming Assembly language of CDC CYBER 170/750 computer

    International Nuclear Information System (INIS)

    Aiming to optimize processing time of BCG computer code in the CDC CYBER 170/750 computer, the FORTRAN-V language of INTERP subroutine was converted to Assembly language. The BCG code was developed for solving neutron transport equation by iterative method, and the INTERP subroutine is innermost loop of the code carrying out 5 interpolation types. The central processor unit Assembly language of the CDC CYBER 170/750 computer and its application in implementing the interpolation subroutine of BCG code are described. (M.C.K.)

  1. An adolescent case of familial hyperparathyroidism with a germline frameshift mutation of the CDC73 gene.

    Science.gov (United States)

    Takeuchi, Takako; Yoto, Yuko; Tsugawa, Takeshi; Kamasaki, Hotaka; Kondo, Atsushi; Ogino, Jiro; Hasegawa, Tadashi; Yama, Naoya; Anan, Sawa; Uchino, Shinya; Ishikawa, Aki; Sakurai, Akihiro; Tsutsumi, Hiroyuki

    2015-10-01

    A 13-yr-old boy who complained of persistent nausea, vomiting and weight loss had hypercalcemia and an elevated intact PTH level. Computed tomography confirmed two tumors in the thyroid gland. The tumors were surgically removed and pathologically confirmed as parathyroid adenoma. Because his maternal aunt and grandmother both had histories of parathyroid tumors, genetic investigation was undertaken for him, and a germline frameshift mutation of the CDC73 gene was identified. CDC73 gene analysis should be done on individuals who are at risk of familial hyperparathyroidism, including those who are asymptomatic, and they should be followed for potential primary hyperparathyroidism and associated disorders including resultant parathyroid carcinoma. PMID:26568659

  2. Aplicação do Sistema de Inferência Nebuloso na Determinação de Medicina Preventiva no Sistema de Saúde Complementar

    Directory of Open Access Journals (Sweden)

    Igor Barone de Medeiros

    2006-12-01

    Full Text Available A aplicação do Sistema de Inferência Nebuloso foi realizada na tentativa de direcionar os custos médicos de operadoras de planos de saúde mediante o estudo de variáveis, previamente, selecionadas por grupos médicos de atuação no mercado. Esta medida irá influenciar as decisões pós entrevista qualificada através do mapeamento do risco dos novos clientes. Este estudo tangibiliza a atual situação do mercado de saúde privada no Brasil, tendo como premissa os escassos recursos da Saúde Pública. Em conseqüência há a tentativa do governo em atender a todos de maneira igualitária, contudo os benefícios a serem oferecidos são, infelizmente, segmentados por não haver recursos para toda a população. O PIB no Brasil equivale a 60% do investimento norte americano em saúde, fonte do relatório do Banco Mundial de 1993, o que representa uma longa jornada de trabalho de nossa economia para o aumento da viabilidade de sobrevivência neste mercado que esta cada vez mais acirrada devido a competição que se encontra o setor e saúde complementar. O objetivo central é restaurar a capacidade comercial deste mercado engessado. A organização de pensamentos dos administradores de saúde (muitos são médicos ainda está ao redor dos procedimentos médicos, pois são eles que alimentam economicamente os Hospitais. Seguindo este foco, são apresentadas vertentes que caracterizam a prevenção como a maior fomentadora de recursos para ambos, operadoras e hospitais, tendo em vista a distribuição dos custos médicos e conseqüentemente o aumento da capacidade dos hospitais em absorver novas técnicas de tratamento, garantindo maior qualidade de vida para os clientes, além de abrir portas para novas parcerias.

  3. 76 FR 29755 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2011-05-23

    ...), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES) In accordance with section 10(a.... Purpose: The ES will provide counsel to the ACD, CDC, regarding a broad range of public health ethics... meeting; discussion of next steps on addressing potential public health ethical issues associated...

  4. 75 FR 57044 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2010-09-17

    ...), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES) In accordance with section 10(a..., CDC, regarding a broad range of public health ethics questions and issues arising from programs..., local and territorial health departments address ethical issues in the practice of public health;...

  5. 77 FR 2549 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)-Ethics...

    Science.gov (United States)

    2012-01-18

    ...), Centers for Disease Control and Prevention (CDC)--Ethics Subcommittee (ES) In accordance with section 10(a... provide counsel to the ACD, CDC, regarding a broad range of public health ethics questions and issues... health departments in their efforts to address public health ethics challenges, approaches for...

  6. Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells

    DEFF Research Database (Denmark)

    Czuchra, Aleksandra; Wu, Xunwei; Meyer, Hannelore;

    2005-01-01

    of Cdc42 did not affect filopodium or lamellipodium formation and had no significant influence on the speed of directed migration nor on mitosis. Cdc42-deficient cells displayed a more elongated cell shape and had a reduced area. Furthermore, directionality during migration and reorientation of the Golgi...

  7. Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1

    DEFF Research Database (Denmark)

    Petersen, B O; Wagener, C; Marinoni, F;

    2000-01-01

    CDC6 is conserved during evolution and is essential and limiting for the initiation of eukaryotic DNA replication. Human CDC6 activity is regulated by periodic transcription and CDK-regulated subcellular localization. Here, we show that, in addition to being absent from nonproliferating cells, CD...... proteolysis of CDC6 in early G(1) and in quiescent cells suggests that this process is part of a mechanism that ensures the timely licensing of replication origins during G(1).......CDC6 is conserved during evolution and is essential and limiting for the initiation of eukaryotic DNA replication. Human CDC6 activity is regulated by periodic transcription and CDK-regulated subcellular localization. Here, we show that, in addition to being absent from nonproliferating cells, CDC6...... is targeted for ubiquitin-mediated proteolysis by the anaphase promoting complex (APC)/cyclosome in G(1). A combination of point mutations in the destruction box and KEN-box motifs in CDC6 stabilizes the protein in G(1) and in quiescent cells. Furthermore, APC, in association with CDH1, ubiquitinates CDC6...

  8. Cdc7-Dbf4 Kinase Overexpression in Multiple Cancers and Tumor Cell Lines Is Correlated with p53 Inactivation

    Directory of Open Access Journals (Sweden)

    Dorine Bonte

    2008-09-01

    Full Text Available Cdc7 is a conserved serine/threonine kinase essential for the initiation of DNA replication, likely by activating the MCM DNA helicase at the G1- to S-phase transition. Cdc7 kinase activity requires association with its regulatory subunit Dbf4/activator of S-phase kinase. Cdc7-Dbf4 is also downstream of the conserved Ataxia telangectasia and RAD3-related kinase that responds to stalled replication forks or DNA damage. In this study, we found that Cdc7 protein was very low or undetectable in normal tissues and cell lines but had increased expression in ∼50% of the 62 human tumor cell lines we examined. Most cell lines with increased Cdc7 protein levels also had increased Dbf4 abundance, and some tumor cell lines had extra copies of the DBF4 gene. A high expression of Cdc7 protein was also detected in primary breast, colon, and lung tumors but not in the matched normal tissues. We also found a high correlation between p53 loss and increased CDC7 and DBF4 expression in primary breast cancers (P = 3.6 × 10−9 and 1.8 × 10−10, respectively and in the cancer cell lines we studied. Therefore, increased Cdc7-Dbf4 abundance may be a common occurrence in human malignancies.

  9. Make a Difference at Your School! CDC Resources Can Help You Implement Strategies to Prevent Obesity Among Children and Adolescents

    Science.gov (United States)

    Centers for Disease Control and Prevention, 2008

    2008-01-01

    The Centers for Disease Control and Prevention (CDC) reviews scientific evidence to determine which school-based policies and practices are most likely to improve key health behaviors among young people, including physical activity and healthy eating. In this document, the CDC identifies ten strategies to help schools prevent obesity by promoting…

  10. Impacto econômico no tratamento do glaucoma: volume de gotas de colírios antiglaucomatosos brasileiros e norte-americanos Economic impact of glaucoma treatment: Brazilian and North-american antiglaucomatous eyedrop volumes

    Directory of Open Access Journals (Sweden)

    Roberto Roizenblatt

    2001-04-01

    Full Text Available Objetivo: Comparar o exame de gotas de colírios antiglaucomatosos norte-americanos e brasileiros, e a repercussão no custo do tratamento. Métodos: Estudo volumétrico da gota e conseqüente número de gotas por mililitro, com estabelecimento da duração média de cada frasco de colírio para a posologia e custo anual do tratamento. Resultados: A gota de Alphagan® brasileiro foi em média 18% maior que a norte-americana, com duração média de 30,8 dias e custo anual de R$ 440,70; o AlphaganTM norte-americano teve duração média de 36,3 dias e custo anual de R$374,10, ou seja, 17,8% de diferença no custo anual. A gota de Betoptic S® brasileiro foi 38,4% maior que a norte-americana, com duração de 31,3 dias e custo anual de R$ 192,60; o Betoptic S TM norte-americano teve duração de 43,3 dias e custo anual de R$ 139,40, ou seja, 38,1% de diferença no custo anual. A gota de Iopidine® brasileiro foi 46,3% maior que a norte-americana, com duração de 35,7 dias e custo anual de R$ 365,00; o IopidineTM norte-americano teve duração de 52,6 dias e custo anual de R$ 248,40, ou seja, 46,9% de diferença no custo anual. A gota de Timoptol® foi 14,7% maior que a norte-americana, com duração de 45,4 dias e custo anual de R$ 58,40; o TimopticTM norte-americano teve duração de 52,1 dias e custo anual de R$ 51,00, ou seja, 14,5% de diferença no custo anual. Conclusões: Em todos os colírios o volume da gota brasileira foi estatisticamente maior, acarretando menor duração do frasco, maior custo anual do tratamento com desperdício significativo e prejuízo para o consumidor.Purpose: To assess the volume of each drop of Brazilian commercially available antiglaucomatous eyedrops and to compare the results with North-american equivalents in order to evaluate the economic implications. Methods: Volumetric study of drops and related impact in the cost of treatment. Results: Brazilian Alphagan® drop volume was 18% larger and the bottle had a

  11. Regulación neuroendocrina del sistema inmune

    OpenAIRE

    Caballero Hernández, Diana; TAMEZ GUERRA, REYES S.; RODRÍGUEZ PADILLA, CRISTINA; Tamez Guerra, Patricia; Richard J. Weber; Gómez Flores, Ricardo

    2001-01-01

    El sistema inmune recibe señales del sistema nervioso central (cerebro) vía el sistema nervioso autónomo y el sistema endocrino. El sistema inmune, a su vez, envía información al cerebro vía citocinas. Este sistema de retroalimentación es vital para el funcionamiento adecuado del organismo en situaciones normales, y en aquellas en las que la homeostasis se ve perturbada, como en casos de estrés, consumo de drogas (terapéuticas o de abuso), enfermedades infecciosas y...

  12. CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor I with chromatin.

    Science.gov (United States)

    Jeffery, Daniel C B; Kakusho, Naoko; You, Zhiying; Gharib, Marlene; Wyse, Brandon; Drury, Erin; Weinreich, Michael; Thibault, Pierre; Verreault, Alain; Masai, Hisao; Yankulov, Krassimir

    2015-01-01

    Chromatin Assembly Factor I (CAF-I) plays a key role in the replication-coupled assembly of nucleosomes. It is expected that its function is linked to the regulation of the cell cycle, but little detail is available. Current models suggest that CAF-I is recruited to replication forks and to chromatin via an interaction between its Cac1p subunit and the replication sliding clamp, PCNA, and that this interaction is stimulated by the kinase CDC7. Here we show that another kinase, CDC28, phosphorylates Cac1p on serines 94 and 515 in early S phase and regulates its association with chromatin, but not its association with PCNA. Mutations in the Cac1p-phosphorylation sites of CDC28 but not of CDC7 substantially reduce the in vivo phosphorylation of Cac1p. However, mutations in the putative CDC7 target sites on Cac1p reduce its stability. The association of CAF-I with chromatin is impaired in a cdc28-1 mutant and to a lesser extent in a cdc7-1 mutant. In addition, mutations in the Cac1p-phosphorylation sites by both CDC28 and CDC7 reduce gene silencing at the telomeres. We propose that this phosphorylation represents a regulatory step in the recruitment of CAF-I to chromatin in early S phase that is distinct from the association of CAF-I with PCNA. Hence, we implicate CDC28 in the regulation of chromatin reassembly during DNA replication. These findings provide novel mechanistic insights on the links between cell-cycle regulation, DNA replication and chromatin reassembly.

  13. Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction.

    Science.gov (United States)

    Chen, Ying-Chou; Weinreich, Michael

    2010-12-31

    Cdc7-Dbf4 is a conserved, two-subunit kinase required for initiating eukaryotic DNA replication. Recent studies have shown that Cdc7-Dbf4 also regulates the mitotic exit network (MEN) and monopolar homolog orientation in meiosis I (Matos, J., Lipp, J. J., Bogdanova, A., Guillot, S., Okaz, E., Junqueira, M., Shevchenko, A., and Zachariae, W. (2008) Cell 135, 662-678 and Miller, C. T., Gabrielse, C., Chen, Y. C., and Weinreich, M. (2009) PLoS Genet. 5, e1000498). Both activities likely involve a Cdc7-Dbf4 interaction with Cdc5, the single Polo-like kinase in budding yeast. We previously showed that Dbf4 binds the Cdc5 polo-box domain (PBD) via an ∼40-residue N-terminal sequence, which lacks a PBD consensus binding site (S(pS/pT)(P/X)), and that Dbf4 inhibits Cdc5 function during mitosis. Here we identify a non-consensus PBD binding site within Dbf4 and demonstrate that the PBD-Dbf4 interaction occurs via a distinct PBD surface from that used to bind phosphoproteins. Genetic and biochemical analysis of multiple dbf4 mutants indicate that Dbf4 inhibits Cdc5 function through direct binding. Surprisingly, mutation of invariant Cdc5 residues required for binding phosphorylated substrates has little effect on yeast viability or growth rate. Instead, cdc5 mutants defective for binding phosphoproteins exhibit enhanced resistance to microtubule disruption and an increased rate of spindle elongation. This study, therefore, details the molecular nature of a new type of PBD binding and reveals that Cdc5 targeting to phosphorylated substrates likely regulates spindle dynamics.

  14. CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor i with chromatin

    Science.gov (United States)

    Jeffery, Daniel CB; Kakusho, Naoko; You, Zhiying; Gharib, Marlene; Wyse, Brandon; Drury, Erin; Weinreich, Michael; Thibault, Pierre; Verreault, Alain; Masai, Hisao; Yankulov, Krassimir

    2015-01-01

    Chromatin Assembly Factor I (CAF-I) plays a key role in the replication-coupled assembly of nucleosomes. It is expected that its function is linked to the regulation of the cell cycle, but little detail is available. Current models suggest that CAF-I is recruited to replication forks and to chromatin via an interaction between its Cac1p subunit and the replication sliding clamp, PCNA, and that this interaction is stimulated by the kinase CDC7. Here we show that another kinase, CDC28, phosphorylates Cac1p on serines 94 and 515 in early S phase and regulates its association with chromatin, but not its association with PCNA. Mutations in the Cac1p-phosphorylation sites of CDC28 but not of CDC7 substantially reduce the in vivo phosphorylation of Cac1p. However, mutations in the putative CDC7 target sites on Cac1p reduce its stability. The association of CAF-I with chromatin is impaired in a cdc28–1 mutant and to a lesser extent in a cdc7–1 mutant. In addition, mutations in the Cac1p-phosphorylation sites by both CDC28 and CDC7 reduce gene silencing at the telomeres. We propose that this phosphorylation represents a regulatory step in the recruitment of CAF-I to chromatin in early S phase that is distinct from the association of CAF-I with PCNA. Hence, we implicate CDC28 in the regulation of chromatin reassembly during DNA replication. These findings provide novel mechanistic insights on the links between cell-cycle regulation, DNA replication and chromatin reassembly. PMID:25602519

  15. CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor I with chromatin.

    Science.gov (United States)

    Jeffery, Daniel C B; Kakusho, Naoko; You, Zhiying; Gharib, Marlene; Wyse, Brandon; Drury, Erin; Weinreich, Michael; Thibault, Pierre; Verreault, Alain; Masai, Hisao; Yankulov, Krassimir

    2015-01-01

    Chromatin Assembly Factor I (CAF-I) plays a key role in the replication-coupled assembly of nucleosomes. It is expected that its function is linked to the regulation of the cell cycle, but little detail is available. Current models suggest that CAF-I is recruited to replication forks and to chromatin via an interaction between its Cac1p subunit and the replication sliding clamp, PCNA, and that this interaction is stimulated by the kinase CDC7. Here we show that another kinase, CDC28, phosphorylates Cac1p on serines 94 and 515 in early S phase and regulates its association with chromatin, but not its association with PCNA. Mutations in the Cac1p-phosphorylation sites of CDC28 but not of CDC7 substantially reduce the in vivo phosphorylation of Cac1p. However, mutations in the putative CDC7 target sites on Cac1p reduce its stability. The association of CAF-I with chromatin is impaired in a cdc28-1 mutant and to a lesser extent in a cdc7-1 mutant. In addition, mutations in the Cac1p-phosphorylation sites by both CDC28 and CDC7 reduce gene silencing at the telomeres. We propose that this phosphorylation represents a regulatory step in the recruitment of CAF-I to chromatin in early S phase that is distinct from the association of CAF-I with PCNA. Hence, we implicate CDC28 in the regulation of chromatin reassembly during DNA replication. These findings provide novel mechanistic insights on the links between cell-cycle regulation, DNA replication and chromatin reassembly. PMID:25602519

  16. A novel functional polymorphism in the Cdc6 promoter is associated with the risk for hepatocellular carcinoma

    International Nuclear Information System (INIS)

    Cdc6 is essential for DNA replication and its deregulation is involved in carcinogenesis. To date, the biological significance of the polymorphism in Cdc6 promoter is still unknown. In this study, we aimed to evaluate the influence of the Cdc6 -515A>G polymorphism (rs4134994) on the individual's susceptibility to cancer and on the function of Cdc6. The Cdc6 -515A>G polymorphism was genotyped in 387 hepatocellular carcinoma (HCC) and 389 age- and sex-matched healthy subjects. The association between the genotypes and the risk for HCC was then estimated by unconditional logistic regression analysis with adjustment for age, sex and HBV status. Compared with the AA homozygotes, the homozygous GG genotype (adjusted OR = 0.36, 95% confidence interval (CI) = 0.18-0.72, P = 0.004) or the combined AG/GG genotypes (adjusted OR = 0.56, 95% CI = 0.36-0.86, P = 0.008) were statistically significantly associated with the reduced risk for HCC. Moreover, the analysis using luciferase reporter system showed that the G-allelic Cdc6 promoter displayed a decreased transcriptional activity compared with the A-allelic one. These results indicate that the individuals with G allele may have reduced Cdc6 expression and are therefore in reduced risk for HCC. Further investigation using electrophoretic mobility shift assay (EMSA) revealed that the G allele had a stronger binding strength to nuclear protein(s) which might function as negative regulator(s) for Cdc6 transcription. Our findings suggest that the -515A>G polymorphism may affect the Cdc6 promoter binding affinity with nuclear protein(s) and in turn the Cdc6 expression, which consequently modulates the individual's susceptibility to HCC

  17. Diagnóstico del sistema financiero, la banca comercial y el sistema de portafolios basado en el modelo de sistemas viables

    OpenAIRE

    Beatriz Trueba-Ríos; Isaías Badillo-Piña; Oswaldo Morales-Matamoros

    2012-01-01

    Se presenta en este artículo el desarrollo del diagnóstico para el sistema financiero, la banca comercial y el sistema de portafolios para lo cual se hace uso del modelo de sistemas viables de Beer [1]. El sistema financiero ha sido impactado por movimientos adversos del mercado, lo que lo hace dinámico. Haciendo una revisión minuciosa de las etapas de crisis por las que ha pasado el sistema financiero en forma global, se ha observado que la intervención del factor humano en la toma de decisi...

  18. Divergent functions of the Rho GTPases Rac1 and Cdc42 in podocyte injury

    DEFF Research Database (Denmark)

    Blattner, Simone M; Hodgin, Jeffrey B; Nishio, Masashi;

    2013-01-01

    Podocytes are highly specialized epithelial cells with complex actin cytoskeletal architecture crucial for maintenance of the glomerular filtration barrier. The mammalian Rho GTPases Rac1 and Cdc42 are molecular switches that control many cellular processes, but are best known for their roles in ...

  19. Potential utility of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism: a case report

    Science.gov (United States)

    Sato, Takeshi; Muroya, Koji; Hanakawa, Junko; Yamashita, Sumimasa; Nozawa, Kumiko; Masudo, Katsuhiko; Yamakawa, Tadashi; Asakura, Yumi; Hasegawa, Tomonobu; Adachi, Masanori

    2016-01-01

    Abstract. We report a Japanese pedigree with familial primary hyperparathyroidism due to a CDC73 mutation. To our knowledge, this is the first report of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism. The proband had severe psychomotor retardation and received laryngotracheal separation surgery. At 19 yr of age, he developed acute pancreatitis. Hypercalcemia (12.2–13.8 mg/dL), elevated levels of intact PTH (86–160 pg/mL), and a tumor detected upon neck ultrasonography led to the diagnosis of primary hyperparathyroidism. Family history and biochemical examinations revealed that three family members (the proband’s mother, elder brother, and maternal grandfather) had primary hyperparathyroidism. We identified a novel heterozygous mutation, c.240delT, p.Glu81Lysfs*28, in the CDC73 gene in three affected family members, excluding the proband’s elder brother who refused genetic testing. Parathyroidectomy for the proband was considered as high-risk, because the tumor was located close to the tracheostomy orifice. After receiving approval from the institutional review board and obtaining the consent, we initiated cinacalcet treatment. At 22 yr of age, treatment with 100 mg of cinacalcet maintained serum calcium levels below 11.0 mg/dL with no apparent side effects. Our report presents the potential efficacy of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism, in particularly inoperative cases. PMID:27507909

  20. Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin A

    NARCIS (Netherlands)

    Wolthuis, Rob; Clay-Farrace, Lori; van Zon, Wouter; Yekezare, Mona; Koop, Lars; Ogink, Janneke; Medema, Rene; Pines, Jonathon

    2008-01-01

    Successful mitosis requires the right protein be degraded at the right time. Central to this is the spindle checkpoint that prevents the destruction of securin and cyclin 131 when there are improperly attached chromosomes. The principal target of the checkpoint is Cdc20, which activates the anaphase

  1. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1

    NARCIS (Netherlands)

    Lee, S.B.; Kim, J.J.; Nam, H.J.; Gao, B.; Yin, P.; Qin, B.; Yi, S.Y.; Ham, H.; Evans, D.; Kim, S.H.; Zhang, Jun; Deng, M.; Liu, T.; Zhang, H.; Billadeau, D.D.; Wang, L.; Giaime, E.; Shen, J.; Pang, Y.P.; Jen, J.; Deursen, J.M.A. van; Lou, Z.

    2015-01-01

    Mutations in the E3 ubiquitin ligase Parkin have been linked to familial Parkinson's disease. Parkin has also been implicated in mitosis through mechanisms that are unclear. Here we show that Parkin interacts with anaphase promoting complex/cyclosome (APC/C) coactivators Cdc20 and Cdh1 to mediate th

  2. 75 FR 39264 - CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment

    Science.gov (United States)

    2010-07-08

    ... CDC/HRSA Advisory Committee on HIV and STD Prevention and Treatment In accordance with section 10(a)(2... health and STD disparities among racial and ethnic minorities. The objectives of the workgroup are: (1..., Viral Hepatitis, STD, and TB ] Prevention, 1600 Clifton Road, NE., Mailstop E-07, Atlanta, Georgia...

  3. CDC Vital Signs–Think Sepsis. Time Matters.

    Centers for Disease Control (CDC) Podcasts

    2016-08-23

    This podcast is based on the August 2016 CDC Vital Signs report. Sepsis is a medical emergency and can happen quickly. Learn the signs of sepsis and how to prevent it.  Created: 8/23/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 8/23/2016.

  4. Defective tubulin organization and proplatelet formation in murine megakaryocytes lacking Rac1 and Cdc42

    DEFF Research Database (Denmark)

    Pleines, Irina; Dütting, Sebastian; Cherpokova, Deya;

    2013-01-01

    normally in vivo but displayed highly abnormal morphology and uncontrolled fragmentation. Consistently, a lack of Rac1/Cdc42 virtually abrogated proplatelet formation in vitro. Strikingly, this phenotype was associated with severely defective tubulin organization, whereas actin assembly and structure were...

  5. Cdc42 controls progenitor cell differentiation and beta-catenin turnover in skin

    DEFF Research Database (Denmark)

    Wu, Xunwei; Quondamatteo, Fabio; Lefever, Tine;

    2006-01-01

    Differentiation of skin stem cells into hair follicles (HFs) requires the inhibition of beta-catenin degradation, which is controlled by a complex containing axin and the protein kinase GSK3beta. Using conditional gene targeting in mice, we show now that the small GTPase Cdc42 is crucial...

  6. Cdc42-dependent structural development of auditory supporting cells is required for wound healing at adulthood

    DEFF Research Database (Denmark)

    Anttonen, Tommi; Kirjavainen, Anna; Belevich, Ilya;

    2012-01-01

    of a basolateral membrane protein in the apical domain were observed. These defects and changes in aPKCλ/ι expression suggested that apical polarization is impaired. Following a lesion at adulthood, supporting cells with Cdc42 loss-induced maturational defects collapsed and failed to remodel F-actin belts...

  7. On-Line Help: Design Issues for Authoring Systems. CDC Technical Report No. 18.

    Science.gov (United States)

    Duffy, Thomas M.; Langston, M. Diane

    Part of the Communications Design Center's (CDC) on-going research on communication and document design, this report emphasizes the importance of a multi-level computer help system that goes beyond simple command descriptions to include assistance with goals and strategies within the application domain--in this case, automated authoring. The help…

  8. Potential utility of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism: a case report.

    Science.gov (United States)

    Sato, Takeshi; Muroya, Koji; Hanakawa, Junko; Yamashita, Sumimasa; Nozawa, Kumiko; Masudo, Katsuhiko; Yamakawa, Tadashi; Asakura, Yumi; Hasegawa, Tomonobu; Adachi, Masanori

    2016-07-01

    We report a Japanese pedigree with familial primary hyperparathyroidism due to a CDC73 mutation. To our knowledge, this is the first report of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism. The proband had severe psychomotor retardation and received laryngotracheal separation surgery. At 19 yr of age, he developed acute pancreatitis. Hypercalcemia (12.2-13.8 mg/dL), elevated levels of intact PTH (86-160 pg/mL), and a tumor detected upon neck ultrasonography led to the diagnosis of primary hyperparathyroidism. Family history and biochemical examinations revealed that three family members (the proband's mother, elder brother, and maternal grandfather) had primary hyperparathyroidism. We identified a novel heterozygous mutation, c.240delT, p.Glu81Lysfs*28, in the CDC73 gene in three affected family members, excluding the proband's elder brother who refused genetic testing. Parathyroidectomy for the proband was considered as high-risk, because the tumor was located close to the tracheostomy orifice. After receiving approval from the institutional review board and obtaining the consent, we initiated cinacalcet treatment. At 22 yr of age, treatment with 100 mg of cinacalcet maintained serum calcium levels below 11.0 mg/dL with no apparent side effects. Our report presents the potential efficacy of cinacalcet as a treatment for CDC73-related primary hyperparathyroidism, in particularly inoperative cases. PMID:27507909

  9. Opioid Epidemic Costs U.S. $78.5 Billion Annually: CDC

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_161089.html Opioid Epidemic Costs U.S. $78.5 Billion Annually: CDC ... HealthDay News) -- Abuse of powerful prescription painkillers called opioids costs the U.S. economy $78.5 billion a ...

  10. Using Evidence-Based Parenting Programs to Advance CDC Efforts in Child Maltreatment Prevention. Research Brief

    Science.gov (United States)

    Valle, Linda Anne; Whitaker, Daniel J.; Lutzker, John R.; Filene, Jill H.; Wyatt, Jennifer M.; Cephas, Kendell C.; Hoover, D. Michele

    2004-01-01

    The Centers for Disease Control and Prevention (CDC) recognize child maltreatment as a serious public health problem with extensive short- and long-term health effects. In addition to the immediate physical and emotional effects of maltreatment, children who have experienced abuse and neglect are at increased risk of adverse health effects and…

  11. 76 FR 62071 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2011-10-06

    ... HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) In accordance with section 10(a)(2) of the Federal..., for both the Centers for Disease Control and Prevention and the Agency for Toxic Substances...

  12. 77 FR 19018 - Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC)

    Science.gov (United States)

    2012-03-29

    ... HUMAN SERVICES Centers for Disease Control and Prevention Advisory Committee to the Director (ACD), Centers for Disease Control and Prevention (CDC) In accordance with section 10(a)(2) of the Federal... and other committee management activities, for both the Centers for Disease Control and Prevention...

  13. Modulation of yeast telomerase activity by Cdc13 and Est1 in vitro

    Science.gov (United States)

    Chen, Yu-Fan; Lu, Chia-Ying; Lin, Yi-Chien; Yu, Tai-Yuan; Chang, Chun-Ping; Li, Jing-Ru; Li, Hung-Wen; Lin, Jing-Jer

    2016-01-01

    Telomerase is the enzyme involved in extending telomeric DNA. Control of telomerase activity by modulating its access to chromosome ends is one of the most important fundamental mechanisms. This study established an in vitro yeast telomerase reconstitution system that resembles telomere replication in vivo. In this system, a tailed-duplex DNA formed by telomeric DNA was employed to mimic the structure of telomeres. The core catalytic components of telomerase Est2/Tlc1 RNA were used as the telomeric DNA extension machinery. Using the reconstituted systems, this study found that binding of Cdc13 to telomeric DNA inhibited the access of telomerase to its substrate. The result was further confirmed by a single-molecule approach using the tethered-particle motion (TPM)-based telomerase assay. The findings also showed that the inhibitory effect can be relieved by telomerase-associated protein Est1, consistent with the role of Cdc13 and Est1 in regulating telomere extension in vivo. Significantly, this study found that the DNA binding property of Cdc13 was altered by Est1, providing the first mechanistic evidence of Est1 regulating the access of telomerase to its substrate. Thus, the roles of Cdc13 and Est1 in modulating telomerase activity were clearly defined using the in vitro reconstituted system. PMID:27659693

  14. 1 in 8 U.S. Workers Has Some Hearing Loss: CDC

    Science.gov (United States)

    ... gov/news/fullstory_158434.html 1 in 8 U.S. Workers Has Some Hearing Loss: CDC Survey finds ... 21, 2016 (HealthDay News) -- Nearly 13 percent of U.S. workers suffer from at least some hearing loss, ...

  15. Frequent alterations of SLIT2-ROBO1-CDC42 signalling pathway in breast cancer: clinicopathological correlation.

    Science.gov (United States)

    Bhattacharya, Rittwika; Mukherjee, Nupur; Dasgupta, Hemantika; Islam, Md Saimul; Alam, Neyaz; Roy, Anup; Das, Priyobrata; Roychoudhury, Susanta; Panda, Chinmay Kumar

    2016-09-01

    The aim of the study was to understand the role of SLIT2-ROBO1/2-CDC42 signalling pathways in development of breast cancer (BC). Primary BC samples (n = 150), comprising of almost equal proportion of four subtypes were tested for molecular alterations of SLIT2, ROBO1, ROBO2 and CDC42, the key regulator genes of this pathway. Deletion and methylation frequencies of the candidate genes were seen in the following order: deletion, SLIT2 (38.6%) > ROBO1 (30%) > ROBO2 (7.3%); methylation, SLIT2 (63.3%) > ROBO1 (26.6%) >ROBO2 (9.3%). Majority (80%, 120/150) of the tumours showed alterations (deletion/methylation) in at least one of the candidate genes. Overall, alterations of the candidate genes were as follows: SLIT2, 75.3% (101/150); ROBO1, 45.3% (68/150); ROBO2, 15.3% (23/150). Significantly, higher alteration of SLIT2 locus was observed in triple negative breast cancer (TNBC) over HER2 subtype (P = 0.0014). Similar trend is also seen in overall alterations of SLIT2 and/or ROBO1, in TNBC than HER2 subtype (P = 0.0012); of SLIT2 and/or ROBO2 in TNBC than luminal A (P = 0.014) and HER2 subtype (P = 0.048). Immunohistochemical analysis of SLIT2, ROBO1/2 showed reduced expression, concordant with their molecular alterations. Also, high expression of total CDC42 (49/52; 94.2%) and reduced expression of phospho Serine-71 CDC42 (41/52; 78.8%) was observed. Coalterations of SLIT2 and/or ROBO1, SLIT2 and/or ROBO2 had significant association with reduced expression of phospho Serine-71 CDC42 (P = 0.0012-0.0038). Alterations of SLIT2 and/or ROBO1, reduced expression of phospho Serine-71 CDC42 predicted poor survival of BC patients. Results indicate the importance of SLIT2-ROBO1-CDC42 signalling pathway in predicting tumour progression.

  16. Molecular and structural insight into lysine selection on substrate and ubiquitin lysine 48 by the ubiquitin-conjugating enzyme Cdc34

    DEFF Research Database (Denmark)

    Suryadinata, Randy; Holien, Jessica K; Yang, George;

    2013-01-01

    the native salt-bridge interactions in Ub and Cdc34, resulting in misplacement of Sic1 lysine 50 in the Cdc34 catalytic cleft. During polyubiquitination, Cdc34 showed a strong preference for Ub lysine 48 (K48), with lower activity towards lysine 11 (K11) and lysine 63 (K63). Mutating the -2, -1, +1 and +2...

  17. Contexto General de los Sistemas Agrarios

    Directory of Open Access Journals (Sweden)

    Vélez Escobar Norberto

    1989-12-01

    Full Text Available Esta ponencia introdujo y delimitó el campo de trabajo para el panel: Formas o Sistemas de Producción Agraria. En ella se presentó y discutió la definición del concepto de FORMA DE PRODUCCION e igualmente planteó la taxonomía más general o "agrupamientos" de los sistemas de producción agraria en Colombia. Se identificaron cinco grupos de formas, en proceso de estructuración, empresariales, campesinas, de economía natural y experimentales; y a continuación se caracterizó y explicó cada uno de los cinco agrupamientos. La ponencia terminó sustentando la validez del trabajo académico basado en el enfoque de sistemas y presentó la evolución reciente de esta forma de trabajo en la Universidad Nacional, Seccional Medellín.

  18. SCODA para el Desarrollo de Sistemas Multiagente

    Directory of Open Access Journals (Sweden)

    Jesús A. Román

    2011-12-01

    Full Text Available El planteamiento de implementar sistemas multiagente de forma que puedan reutilizarse con distintos objetivos, dota a los desarrolladores de una metodología más eficaz en sus fines. Este artículo presenta SCODA, una arquitectura basada en cinco principios: estandaridad, especialización, facilidad de implementación, reutilización y computación distribuida. SCODA pretende facilitar el desarrollo de sistemas multiagente, basándose en el concepto de pequeños grupos de agentes denominados Comunidades Inteligentes Especializadas (CIE. Las CIE cuentan con funcionalidades específicas que permiten llevar a cabo la implementación de sistemas multiagente de forma escalable, dentro del marco de SCODA, pudiendo ser reutilizadas en diversos desarrollos.

  19. Cdc20 is critical for meiosis I and fertility of female mice.

    Directory of Open Access Journals (Sweden)

    Fang Jin

    2010-09-01

    Full Text Available Chromosome missegregation in germ cells is an important cause of unexplained infertility, miscarriages, and congenital birth defects in humans. However, the molecular defects that lead to production of aneuploid gametes are largely unknown. Cdc20, the activating subunit of the anaphase-promoting complex/cyclosome (APC/C, initiates sister-chromatid separation by ordering the destruction of two key anaphase inhibitors, cyclin B1 and securin, at the transition from metaphase to anaphase. The physiological significance and full repertoire of functions of mammalian Cdc20 are unclear at present, mainly because of the essential nature of this protein in cell cycle progression. To bypass this problem we generated hypomorphic mice that express low amounts of Cdc20. These mice are healthy and have a normal lifespan, but females produce either no or very few offspring, despite normal folliculogenesis and fertilization rates. When mated with wild-type males, hypomorphic females yield nearly normal numbers of fertilized eggs, but as these embryos develop, they become malformed and rarely reach the blastocyst stage. In exploring the underlying mechanism, we uncover that the vast majority of these embryos have abnormal chromosome numbers, primarily due to chromosome lagging and chromosome misalignment during meiosis I in the oocyte. Furthermore, cyclin B1, cyclin A2, and securin are inefficiently degraded in metaphase I; and anaphase I onset is markedly delayed. These results demonstrate that the physiologically effective threshold level of Cdc20 is high for female meiosis I and identify Cdc20 hypomorphism as a mechanism for chromosome missegregation and formation of aneuploid gametes.

  20. Distribuição da altura uterina ao longo da gestação em uma coorte brasileira: comparação com a curva de referência do Centro Latino-Americano de Perinatologia Distribution of uterine height during pregnancy in a Brazilian cohort: comparison with the reference curve of the Centro Latino-Americano de Perinatologia

    Directory of Open Access Journals (Sweden)

    Maria Lúcia Rocha Oppermann

    2006-09-01

    Full Text Available OBJETIVOS: descrever, em gestantes do Estudo Brasileiro do Diabetes Gestacional (EBDG, a distribuição da altura uterina de acordo com a idade gestacional e validar a curva do Centro Latino-Americano de Perinatologia (CLAP, curva de referência para a predição de anormalidades do crescimento fetal. MÉTODOS: o EBDG é uma coorte de 5564 gestantes, com mais de 19 anos, seguidas até e após o parto. Entrevistas e medidas antropométricas padronizadas foram feitas no arrolamento entre a 20ª e a 28ª semana. Os prontuários foram revisados segundo protocolo padronizado, abrangendo os períodos de pré-natal e parto. As análises referem-se a 3539 gestantes com datação da gravidez confirmada por ultra-sonografia. Determinamos as propriedades diagnósticas dos percentis 10 e 90 de altura uterina de ambas as curvas (EBDG e CLAP como indicadores de anormalidade no peso neonatal. RESULTADOS: as medidas de altura uterina no EBDG foram maiores que as do CLAP em todas as semanas de gestação (1 a 4 cm e 2 a 6 cm, respectivamente, nos percentis 10 e 90. O percentil 10 do CLAP identificou como pequenas as medidas uterinas de 0,3 a 1,7% das gestantes brasileiras, ao passo que o percentil 90 classificou como grandes as medidas uterinas de 42 a 57% das brasileiras. A sensibilidade do percentil 10 do CLAP para predizer recém-nascidos pequenos para a idade gestacional variou de 0,8 a 6% e a especificidade do percentil 90 para predizer grandes para a idade gestacional, de 46 a 61%. CONCLUSÕES: a curva de referência do CLAP não reflete o padrão de crescimento uterino das gestantes brasileiras, limitando sua capacidade de identificar anormalidades de crescimento fetal, especialmente a restrição de crescimento.PURPOSE: to describe, in participants of the Brazilian Study of Gestational Diabetes (EBDG, the percentile distribution of uterine height by gestational age and to validate the use of percentiles of the chart derived by the "Centro Latino-Americano

  1. Educação bilíngüe e multiculturalismo: o exemplo americano = Bilingual education and multiculturalism: the american example

    Directory of Open Access Journals (Sweden)

    Naiditch, Fernando

    2007-01-01

    Full Text Available Este artigo apresenta uma visão histórica de educação bilingüe nos Estados Unidos e mostra que a forma como ela foi compreendida mudou através dos anos. O artigo focaliza as diferentes políticas educacionais e a legislação que regulamentam o uso de outras linguas que não o inglês no sistema educacional público e privado nos EUA. Políticas públicas que tratam do ensino de inglês como segunda língua também são discutidas. A perspectiva histórica apresentada no artigo percorre diferentes momentos da história americana e mostra como eles afetaram as políticas educacionais que guiam o uso do inglês e de linguas estrangeiras nas escolas (ex: imigração, o Sputnik, o movimento de direitos civis, e mais recentemente o Nenhuma Criança Deixada para Trás. O artigo mostra as vantagens de se viver em uma sociedade multicultural, e também como o sistema de educação pública pode se beneficiar deste fato, e como administradores e educadores devem abordar diversidade lingüística e cultural tanto dentro como fora da sala de aula

  2. Sistema educativo y mercado de trabajo 

    OpenAIRE

    López Peñate, Celedonio

    1999-01-01

    [ES] Estudio de las condiciones que propiciaron el salto cualitativo producido en el desarrollo del Sistema Educativo canario a finales de los setenta, y aproximación a una evaluación de dicho sistema.

  3. El Derecho Administrativo del Sistema Comunitario Andino

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Santos Rodríguez

    2013-12-01

    Full Text Available Una de las tendencias contemporáneas del Derecho Administrativo es el reconocimiento de su existencia más allá de las fronteras de un Estado. Bajo esa premisa, se busca demostrar que en la Comunidad Andina de Naciones se configuran los elementos suficientes para afirmar que existe un Derecho Administrativo Comunitario Andino, en la medida en que de las normas andinas se desprende la existencia de una función administrativa andina, una organización administrativa al seno del Sistema Andino de Integración, un sistema de fuentes administrativas andina y un contencioso administrativo comunitario.

  4. El Sistema Político Latinoamericano

    OpenAIRE

    Luis Dallanegra Pedraza

    2003-01-01

    Se ha escrito mucho sobre la problemática latinoamericana, y sobre sus aspectos históricos, especialmente en relación con Estados Unidos, y sobre las políticas que este país ha ido aplicando históricamente en sus sucesivos períodos de gobierno, hacia América Latina. Sin embargo, no se ha hecho un estudio profundo sobre el sistema político latinoamericano. Al menos sobre la existencia de un sistema político específico en América Latina que la caracterice desde ese punto de vista...

  5. Sistemas de Gestión Distribuidos

    OpenAIRE

    Pesado, Patricia; Bertone, Rodolfo Alfredo; Boracchia, Marcos; Thomas, Pablo Javier; Pasini, Ariel C.; Ricci, Guillermo; Labataglia, J.; De Giusti, Armando Eduardo

    2003-01-01

    Esta línea de I/D se refiere a realizar investigación y desarrollo en temas de Sistemas de Software Distribuido, particularmente los relacionados con los aspectos de Ingeniería de Software que se orientan al desarrollo e implementación de proyectos de sistemas distribuidos. Estas tareas abarcan aspectos propios de la arquitectura, comunicaciones, estructuración de bases de datos, así como especificación, validación y desarrollo de aplicaciones de software en ambientes distribuidos. Asimism...

  6. CATEGORIZANDO A LOS USUARIOS DE SISTEMAS DIGITALES.

    OpenAIRE

    Denise Hernández y Hernández; Alberto Ramírez-Martinell; Daniel Cassany

    2014-01-01

    Repasamos las categorías más utilizadas para aludir a las características de las personas que utilizan algún tipo de sistema digital, ya sea por su grado de participación en la comu- nicación global y en los sistemas web -1.0 o 2.0-; por la actitud que toman ante el contenido digital -consumidores o productores-; por su intermitencia en el mundo digital -residentes o visitantes-; o por el momento histórico en el que nacieron -nativos digitales o inmigrantes digitales-, con el propósito de ref...

  7. Estudo comparativo de sistemas em aquecimento

    OpenAIRE

    Castro, Inês Marques Costa Borges de

    2013-01-01

    O presente estudo pretende avaliar o desempenho energético e rentabilidade do uso de equipamento de climatização para o aquecimento ambiente de uma casa sénior, através de sistemas tradicionais de produção de água aquecida, como a caldeira e a tradicional bomba de calor, e um sistema apoiado num equipamento de produção solar térmico. Perante uma determinada arquitectura, foram calculados os caudais de ar novo, de forma a garantir a qualidade do ar interior, cumprindo os requisitos da actua...

  8. Sistema de gestión documental

    OpenAIRE

    Fernández Gómez, Javier

    2015-01-01

    Esta memoria expone la planificación, el análisis, el diseño y el desarrollo de un sistema de gestión documental, en adelante SGD, configurable, es decir, adaptable a un ámbito determinado. El SGD consta de dos grupos de interfaces o funcionalidades claramente diferenciadas: administración y gestión documental. Aquesta memòria exposa la planificació, l'anàlisi, el disseny i el desenvolupament d'un sistema de gestió documental, d'ara endavant SGA, configurable, és a dir, adaptable a un àmbi...

  9. Sistemas de Indicadores de Desempenho em Projetos

    OpenAIRE

    Julia Garaldi Borges; Marly Monteiro de Carvalho

    2011-01-01

    O presente trabalho visa propor um sistema de indicadores de desempenho de projetos, a partir da análise da importância dos critérios de sucesso em projetos. A abordagem metodológica foi a de pesquisa-ação desenvolvida em uma empresa de engenharia, com atuação em diversos setores. Foram utilizadas várias fontes de evidência, tais como análise do atual sistema de indicadores da empresa, análise dos projetos nas bases de dados e entrevistas com representantes do escritório de projetos e com ger...

  10. El sistema linfático: el gran olvidado del sistema circulatorio.

    OpenAIRE

    Olmos Martínez, Susana; Gavidia Catalán, Valentín

    2014-01-01

    En el presente artículo se analizan los modelos mentales y las concepciones alternativas que tienen los estudiantes de distintos niveles educativos sobre el sistema linfático como componente del sistema circulatorio. Se establece una relación con las dificultades que se salvaron a lo largo de la historia en la adquisición de dichos conocimientos. Los resultados muestran una deficiente relación entre los sistemas linfáticos y sanguíneos, una falta de conocimientos tanto anatómicos como fisioló...

  11. Aplicação de sistemas operativos de tempo real no sistema Galileo

    OpenAIRE

    Guimarães, Ivo Daniel Oliveira

    2008-01-01

    Os sistemas operativos de tempo real têm sido imensamente utilizados no desenvolvimento de aplicações embebidas e/ou críticas. No entanto, todos apresentam problemas de fiabilidade e segurança na sua implementação ao disponibilizarem serviços e funcionalidades que ameaçam a integridade da aplicação e por vezes do próprio sistema operativo. Ao longo do documento serão analisados vários aspectos dos sistemas operativos de tempo real, passando por importantes especificações com...

  12. La morera: una alternativa viable para los sistemas de alimentación animal en el trópico

    Directory of Open Access Journals (Sweden)

    D. García

    2006-01-01

    Full Text Available Se presenta una revisión sobre las principales características de las especies de morera y sus potencialidades como plantas forrajeras en las condiciones tropicales; se hace énfasis en los aspectos relacionados con su taxonomía, distribución geográfica, principales usos, manejo agronómico, composición química, valor nutritivo y principales metabolitos secundarios presentes en su biomasa. Las especies de morera, oriundas del continente euroasiático, pertenecen a la familia Moraceae. En ese sentido, Morus alba y Morus nigra son las mayormente estudiadas por su gran distribución mundial. Desde tiempos ancestrales la morera se ha utilizado en Asia para la alimentación del gusano que produce la seda y los estudios agronómicos realizados en la sericultura han proporcionado numerosas informaciones. Sin embargo, la influencia de los principales factores en la producción de forraje solamente ha sido estudiada en forma intensiva después de su introducción al Continente Americano a partir de 1980. La morera se caracteriza por presentar una sobresaliente producción de biomasa (2-30 tMS/ha/año, contenidos elevados de proteína cruda (>15% MS, adecuada fracción fibrosa (20-40% y digestibilidad entre 70 y 90%. La biomasa contiene agentes pronutricionales (flavonoides, cumarinas, esteroles, saponinas, lectinas y fitoestrógenos. No obstante, los contenidos de estos metabolitos no sobrepasan los niveles críticos establecidos para los rumiantes y solamente los tres últimos grupos presentan potencial antinutricional en monogástricos. En virtud de sus singulares características, las especies de morera constituyen excelentes alternativas alimentarias en los sistemas tropicales de bajos insumos.

  13. Migração internacional recente no amazonas: o caso dos Hispano-americanos Latest international migration in the amazon: the case of Hispanic-Americans

    Directory of Open Access Journals (Sweden)

    Sidney Antônio da Silva

    2011-06-01

    Full Text Available A imigração para o Amazonas continua sendo um campo aberto à pesquisa antropológica, seja aquela de cunho histórico, ainda pouco pesquisada, seja a mais recente, sobretudo a oriunda de países fronteiriços da Região Amazônica. Entretanto, o grande desafio que se coloca aos estudiosos dessa temática é superar a referência teórica que norteou as análises que tiveram como marco explicativo os ciclos econômicos, entre eles o da borracha. No caso da imigração recente para o Amazonas, a tentação é relacioná-la também com o ciclo de desenvolvimento econômico propiciado pela criação da Zona Franca de Manaus em 1967, fator que poderia por si só explicar a presença de novos imigrantes, entre eles os hispano-americanos. Compreender a lógica e as especificidades dessa presença é o que pretendemos discutir neste artigo, que se insere em uma preocupação de pensar a imigração no Amazonas não apenas como um fenômeno demográfico e econômico, mas sobretudo como um processo social de mão dupla, ou seja, que afeta tanto a sociedade de origem quanto a de acolhida, em suas dimensões sociais e culturais, nas relações de gênero e nos processos identitários.Immigration to Amazonas state remains an open field for anthropological research, either that of a historical nature, still poorly researched, or that with a current focus, especially from neighboring countries of the Amazon region. However, the great challenge posed to scholars of the subject is to overcome the theoretical framework that guided analysis based on business cycles, such as rubber. In the case of recent immigration to the Amazonas state, the challenge is also to relate it to the cycle of economic development made possible by the creation of the Manaus Free Trade Zone, in 1967. This factor alone could explain the presence of new immigrants, among them, Hispanics. This article, which intends to discuss the rationale and the specifics of this presence, is immerse

  14. The use of the processor communication of the CDC-1604A/1 and CDC-1604A/2 computers for observation of scanning process on the HPD device by means of display

    International Nuclear Information System (INIS)

    On a particular example of film data processing from the Dubna Magnetic Spectrometer the possibility is shown for using the communication between CDC-1604A/1 and CDC-1604A/2 computers for the joint working of two logically communicated programs. Combination of HPD and graphic display software by means of the processors communication channel provides an opportunity to have a visual display of the film during its scanning, without restrictions on the time of processing. Thus, using the communication between two computers one can gain conditions for handling new tasks unrealizable with one CDC-1604A computer

  15. The tenacious recognition of yeast telomere sequence by Cdc13 is fully exerted by a single OB-fold domain.

    Science.gov (United States)

    Lewis, Karen A; Pfaff, Danielle A; Earley, Jennifer N; Altschuler, Sarah E; Wuttke, Deborah S

    2014-01-01

    Cdc13, the telomere end-binding protein from Saccharomyces cerevisiae, is a multidomain protein that specifically binds telomeric single-stranded DNA (ssDNA) with exquisitely high affinity to coordinate telomere maintenance. Recent structural and genetic data have led to the proposal that Cdc13 is the paralog of RPA70 within a telomere-specific RPA complex. Our understanding of Cdc13 structure and biochemistry has been largely restricted to studies of individual domains, precluding analysis of how each domain influences the activity of the others. To better facilitate a comparison to RPA70, we evaluated the ssDNA binding of full-length S. cerevisiae Cdc13 to its minimal substrate, Tel11. We found that, unlike RPA70 and the other known telomere end-binding proteins, the core Cdc13 ssDNA-binding activity is wholly contained within a single tight-binding oligosaccharide/oligonucleotide/oligopeptide binding (OB)-fold. Because two OB-folds are implicated in dimerization, we also evaluated the relationship between dimerization and ssDNA-binding activity and found that the two activities are independent. We also find that Cdc13 binding exhibits positive cooperativity that is independent of dimerization. This study reveals that, while Cdc13 and RPA70 share similar domain topologies, the corresponding domains have evolved different and specialized functions.

  16. Cdc42-dependent Modulation of Tight Junctions and Membrane Protein Traffic in Polarized Madin-Darby Canine Kidney Cells

    Science.gov (United States)

    Rojas, Raul; Ruiz, Wily G.; Leung, Som-Ming; Jou, Tzuu-Shuh; Apodaca, Gerard

    2001-01-01

    Polarized epithelial cells maintain the asymmetric composition of their apical and basolateral membrane domains by at least two different processes. These include the regulated trafficking of macromolecules from the biosynthetic and endocytic pathway to the appropriate membrane domain and the ability of the tight junction to prevent free mixing of membrane domain-specific proteins and lipids. Cdc42, a Rho family GTPase, is known to govern cellular polarity and membrane traffic in several cell types. We examined whether this protein regulated tight junction function in Madin-Darby canine kidney cells and pathways that direct proteins to the apical and basolateral surface of these cells. We used Madin-Darby canine kidney cells that expressed dominant-active or dominant-negative mutants of Cdc42 under the control of a tetracycline-repressible system. Here we report that expression of dominant-active Cdc42V12 or dominant-negative Cdc42N17 altered tight junction function. Expression of Cdc42V12 slowed endocytic and biosynthetic traffic, and expression of Cdc42N17 slowed apical endocytosis and basolateral to apical transcytosis but stimulated biosynthetic traffic. These results indicate that Cdc42 may modulate multiple cellular pathways required for the maintenance of epithelial cell polarity. PMID:11514615

  17. FLIM FRET Visualization of Cdc42 Activation by Netrin-1 in Embryonic Spinal Commissural Neuron Growth Cones

    Science.gov (United States)

    Rappaz, Benjamin; Lai Wing Sun, Karen; Correia, James P.; Wiseman, Paul W.; Kennedy, Timothy E.

    2016-01-01

    Netrin-1 is an essential extracellular chemoattractant that signals through its receptor DCC to guide commissural axon extension in the embryonic spinal cord. DCC directs the organization of F-actin in growth cones by activating an intracellular protein complex that includes the Rho GTPase Cdc42, a critical regulator of cell polarity and directional migration. To address the spatial distribution of signaling events downstream of netrin-1, we expressed the FRET biosensor Raichu-Cdc42 in cultured embryonic rat spinal commissural neurons. Using FLIM-FRET imaging we detected rapid activation of Cdc42 in neuronal growth cones following application of netrin-1. Investigating the signaling mechanisms that control Cdc42 activation by netrin-1, we demonstrate that netrin-1 rapidly enriches DCC at the leading edge of commissural neuron growth cones and that netrin-1 induced activation of Cdc42 in the growth cone is blocked by inhibiting src family kinase signaling. These findings reveal the activation of Cdc42 in embryonic spinal commissural axon growth cones and support the conclusion that src family kinase activation downstream of DCC is required for Cdc42 activation by netrin-1. PMID:27482713

  18. An Armadillo Motif in Ufd3 Interacts with Cdc48 and is Involved in Ubiquitin Homeostasis and Protein Degradation

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, G.; Li, G; Schindelin, H; Lennarz, W

    2009-01-01

    The yeast AAA-ATPase Cdc48 and the ubiquitin fusion degradation (UFD) proteins play important, evolutionarily conserved roles in ubiquitin dependent protein degradation. The N-terminal domain of Cdc48 interacts with substrate-recruiting cofactors, whereas the C terminus of Cdc48 binds to proteins such as Ufd3 that process substrates. Ufd3 is essential for efficient protein degradation and for maintaining cellular ubiquitin levels. This protein contains an N-terminal WD40 domain, a central ubiquitin-binding domain, and a C-terminal Cdc48-binding PUL domain. The crystal structure of the PUL domain reveals an Armadillo repeat with high structural similarity to importin-a, and the Cdc48-binding site could be mapped to the concave surface of the PUL domain by biochemical studies. Alterations of the Cdc48 binding site of Ufd3 by site-directed mutagenesis resulted in a depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Therefore, our data provide direct evidence that the functions of Ufd3 in ubiquitin homeostasis and protein degradation depend on its interaction with the C terminus of Cdc48.

  19. Functional mapping of the fission yeast DNA polymerase δ B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis

    Directory of Open Access Journals (Sweden)

    Gray Fiona C

    2009-08-01

    Full Text Available Abstract Background DNA polymerase δ plays an essential role in chromosomal DNA replication in eukaryotic cells, being responsible for synthesising the bulk of the lagging strand. In fission yeast, Pol δ is a heterotetrameric enzyme comprising four evolutionarily well-conserved proteins: the catalytic subunit Pol3 and three smaller subunits Cdc1, Cdc27 and Cdm1. Pol3 binds directly to the B-subunit, Cdc1, which in turn binds the C-subunit, Cdc27. Human Pol δ comprises the same four subunits, and the crystal structure was recently reported of a complex of human p50 and the N-terminal domain of p66, the human orthologues of Cdc1 and Cdc27, respectively. Results To gain insights into the structure and function of Cdc1, random and directed mutagenesis techniques were used to create a collection of thirty alleles encoding mutant Cdc1 proteins. Each allele was tested for function in fission yeast and for binding of the altered protein to Pol3 and Cdc27 using the two-hybrid system. Additionally, the locations of the amino acid changes in each protein were mapped onto the three-dimensional structure of human p50. The results obtained from these studies identify amino acid residues and regions within the Cdc1 protein that are essential for interaction with Pol3 and Cdc27 and for in vivo function. Mutations specifically defective in Pol3-Cdc1 interactions allow the identification of a possible Pol3 binding surface on Cdc1. Conclusion In the absence of a three-dimensional structure of the entire Pol δ complex, the results of this study highlight regions in Cdc1 that are vital for protein function in vivo and provide valuable clues to possible protein-protein interaction surfaces on the Cdc1 protein that will be important targets for further study.

  20. Estabilización robusta de sistemas lineales

    Directory of Open Access Journals (Sweden)

    Horacio Leyva Castellanos

    2013-12-01

    Full Text Available En este trabajo se muestra un conjunto de resultados que permiten abordar el problema de la estabilización robusta de una familia de sistemas lineales positivos. Se describen dos nuevas aplicaciones en la teoría de control positivo para sistemas compartimentales y se muestra la aplicación de un teorema de robustez para tales sistemas

  1. The Rho GTPase Cdc42 regulates hair cell planar polarity and cellular patterning in the developing cochlea

    Directory of Open Access Journals (Sweden)

    Anna Kirjavainen

    2015-03-01

    Full Text Available Hair cells of the organ of Corti (OC of the cochlea exhibit distinct planar polarity, both at the tissue and cellular level. Planar polarity at tissue level is manifested as uniform orientation of the hair cell stereociliary bundles. Hair cell intrinsic polarity is defined as structural hair bundle asymmetry; positioning of the kinocilium/basal body complex at the vertex of the V-shaped bundle. Consistent with strong apical polarity, the hair cell apex displays prominent actin and microtubule cytoskeletons. The Rho GTPase Cdc42 regulates cytoskeletal dynamics and polarization of various cell types, and, thus, serves as a candidate regulator of hair cell polarity. We have here induced Cdc42 inactivation in the late-embryonic OC. We show the role of Cdc42 in the establishment of planar polarity of hair cells and in cellular patterning. Abnormal planar polarity was displayed as disturbances in hair bundle orientation and morphology and in kinocilium/basal body positioning. These defects were accompanied by a disorganized cell-surface microtubule network. Atypical protein kinase C (aPKC, a putative Cdc42 effector, colocalized with Cdc42 at the hair cell apex, and aPKC expression was altered upon Cdc42 depletion. Our data suggest that Cdc42 together with aPKC is part of the machinery establishing hair cell planar polarity and that Cdc42 acts on polarity through the cell-surface microtubule network. The data also suggest that defects in apical polarization are influenced by disturbed cellular patterning in the OC. In addition, our data demonstrates that Cdc42 is required for stereociliogenesis in the immature cochlea.

  2. The Tea4-PP1 landmark promotes local growth by dual Cdc42 GEF recruitment and GAP exclusion.

    OpenAIRE

    Kokkoris K.; Gallo Castro D.; Martin S.G.

    2014-01-01

    Cell polarization relies on small GTPases, such as Cdc42, which can break symmetry through self-organizing principles, and landmarks that define the axis of polarity. In fission yeast, microtubules deliver the Tea1-Tea4 complex to mark cell poles for growth, but how this complex activates Cdc42 is unknown. Here, we show that ectopic targeting of Tea4 to cell sides promotes the local activation of Cdc42 and cell growth. This activity requires that Tea4 binds the type I phosphatase (PP1) cataly...

  3. The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing

    DEFF Research Database (Denmark)

    Lischetti, Tiziana; Zhang, Gang; Sedgwick, Garry G;

    2014-01-01

    Improperly attached kinetochores activate the spindle assembly checkpoint (SAC) and by an unknown mechanism catalyse the binding of two checkpoint proteins, Mad2 and BubR1, to Cdc20 forming the mitotic checkpoint complex (MCC). Here, to address the functional role of Cdc20 kinetochore localization...... on the SAC because the IC20BD is also required for efficient SAC silencing. Indeed, the IC20BD can disrupt the MCC providing a mechanism for its role in SAC silencing. We thus uncover an unexpected dual function of the second Cdc20 binding site in BubR1 in promoting both efficient SAC signalling and SAC...

  4. The cell cycle rallies the transcription cycle: Cdc28/Cdk1 is a cell cycle-regulated transcriptional CDK.

    Science.gov (United States)

    Chymkowitch, Pierre; Enserink, Jorrit M

    2013-01-01

    In the budding yeast Saccharomyces cerevisiae, the cyclin-dependent kinases (CDKs) Kin28, Bur1 and Ctk1 regulate basal transcription by phosphorylating the carboxyl-terminal domain (CTD) of RNA polymerase II. However, very little is known about the involvement of the cell cycle CDK Cdc28 in the transcription process. We have recently shown that, upon cell cycle entry, Cdc28 kinase activity boosts transcription of a subset of genes by directly stimulating the basal transcription machinery. Here, we discuss the biological significance of this finding and give our view of the kinase-dependent role of Cdc28 in regulation of RNA polymerase II.

  5. Sistemas territoriales de salud frente al sistema general de seguridad social en salud de Colombia

    OpenAIRE

    Jahir Alexander Gutiérrez Ossa; Rubén Darío Restrepo Avendaño

    2014-01-01

    Introducción: El artículo expone los elementos y fundamentos que podrían avalar la generación de sistemas de seguridad social en salud territorial, en complementación con el sistema general preexistente. Materiales y Métodos: La eclosión generalizada en los sistemas de salud, implica la revisión de los modelos propuestos de carácter central y territorial. El análisis económico institucional, permite analizar las condiciones que tienen los sistemas generales para impactar en los esquemas terri...

  6. Prominin-2 expression increases protrusions, decreases caveolae and inhibits Cdc42 dependent fluid phase endocytosis

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Raman Deep, E-mail: Takhter.Ramandeep@mayo.edu; Schroeder, Andreas S.; Scheffer, Luana; Holicky, Eileen L.; Wheatley, Christine L.; Marks, David L., E-mail: Marks.david@mayo.edu; Pagano, Richard E.

    2013-05-10

    Highlights: •Prominin-2 expression induced protrusions that co-localized with lipid raft markers. •Prominin-2 expression decreased caveolae, caveolar endocytosis and increased pCav1. •Prominin-2 expression inhibited fluid phase endocytosis by inactivation of Cdc42. •These endocytic effects can be reversed by adding exogenous cholesterol. •Caveolin1 knockdown restored fluid phase endocytosis in Prominin2 expressing cells. -- Abstract: Background: Membrane protrusions play important roles in biological processes such as cell adhesion, wound healing, migration, and sensing of the external environment. Cell protrusions are a subtype of membrane microdomains composed of cholesterol and sphingolipids, and can be disrupted by cholesterol depletion. Prominins are pentaspan membrane proteins that bind cholesterol and localize to plasma membrane (PM) protrusions. Prominin-1 is of great interest as a marker for stem and cancer cells, while Prominin-2 (Prom2) is reportedly restricted to epithelial cells. Aim: To characterize the effects of Prom-2 expression on PM microdomain organization. Methods: Prom2-fluorescent protein was transfected in human skin fibroblasts (HSF) and Chinese hamster ovary (CHO) cells for PM raft and endocytic studies. Caveolae at PM were visualized using transmission electron microscopy. Cdc42 activation was measured and caveolin-1 knockdown was performed using siRNAs. Results: Prom2 expression in HSF and CHO cells caused extensive Prom2-positive protrusions that co-localized with lipid raft markers. Prom2 expression significantly decreased caveolae at the PM, reduced caveolar endocytosis and increased caveolin-1 phosphorylation. Prom2 expression also inhibited Cdc42-dependent fluid phase endocytosis via decreased Cdc42 activation. Effects on endocytosis were reversed by addition of cholesterol. Knockdown of caveolin-1 by siRNA restored Cdc42 dependent fluid phase endocytosis in Prom2-expressing cells. Conclusions: Prom2 protrusions primarily

  7. A national, geographic database of CDC-funded HIV prevention services: development challenges and potential applications

    Directory of Open Access Journals (Sweden)

    Fogarty Kieran J

    2005-11-01

    Full Text Available Abstract Background From 2000–2002, the Centers for Disease Control and Prevention (CDC funded a study that was designed to improve the information available to program planners about the geographic distribution of CDC-funded HIV prevention services provided by community-based organizations (CBOs. Program managers at CDC recognized the potential of a geographic information system (GIS to organize and analyze information about HIV prevention services and they made GIS a critical component of the study design. The primary objective of this study was to construct a national, geographically-referenced database of HIV prevention services provided by CDC-funded CBOs. We designed a survey instrument to collect information about the geographic service areas where CBOs provided HIV prevention services, then collected data from CBOs that received CDC funding for these services during fiscal year 2000. We developed a GIS database to link questionnaire responses with GIS map layers in a manner that would incorporate overlapping geographies, risk populations and prevention services. We collected geographic service area data in two formats: 1 geopolitical boundaries and 2 geographic distance. Results The survey response rate was 70.3%, i.e. 1,020 of 1,450 community-based organizations responded. The number of HIV prevention programs administered by each CBO ranged from 1 to 23. The survey provided information about 3,028 prevention programs, including descriptions of intervention types, risk populations, race and ethnicity, CBO location and geographic service area. We incorporated this information into a large GIS database, the HIV Prevention Services Database. The use of geopolitical boundaries provided more accurate results than geographic distance. The use of a reference map with the questionnaire improved completeness, accuracy and precision of service area data. Conclusion The survey instrument design and database development procedures that we used

  8. MRG15 activates the cdc2 promoter via histone acetylation in human cells

    Energy Technology Data Exchange (ETDEWEB)

    Pena, AndreAna N., E-mail: andreana.pena@gmail.com [Sam and Ann Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, TX (United States); Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX (United States); Tominaga, Kaoru; Pereira-Smith, Olivia M. [Sam and Ann Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, TX (United States); Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX (United States)

    2011-07-01

    Chromatin remodeling is required for transcriptional activation and repression. MRG15 (MORF4L1), a chromatin modulator, is a highly conserved protein and is present in complexes containing histone acetyltransferases (HATs) as well as histone deacetylases (HDACs). Loss of expression of MRG15 in mice and Drosophila results in embryonic lethality and fibroblast and neural stem/progenitor cells cultured from Mrg15 null mouse embryos exhibit marked proliferative defects when compared with wild type cells. To determine the role of MRG15 in cell cycle progression we performed chromatin immunoprecipitation with an antibody to MRG15 on normal human fibroblasts as they entered the cell cycle from a quiescent state, and analyzed various cell cycle gene promoters. The results demonstrated a 3-fold increase in MRG15 occupancy at the cdc2 promoter during S phase of the cell cycle and a concomitant increase in acetylated histone H4. H4 lysine 12 was acetylated at 24 h post-serum stimulation while there was no change in acetylation of lysine 16. HDAC1 and 2 were decreased at this promoter during cell cycle progression. Over-expression of MRG15 in HeLa cells activated a cdc2 promoter-reporter construct in a dose-dependent manner, whereas knockdown of MRG15 resulted in decreased promoter activity. In order to implicate HAT activity, we treated cells with the HAT inhibitor anacardic acid and determined that HAT inhibition results in loss of expression of cdc2 mRNA. Further, chromatin immunoprecipitation with Tip60 localizes the protein to the same 110 bp stretch of the cdc2 promoter pulled down by MRG15. Additionally, we determined that cotransfection of MRG15 with the known associated HAT Tip60 had a cooperative effect in activating the cdc2 promoter. These results suggest that MRG15 is acting in a HAT complex involving Tip60 to modify chromatin via acetylation of histone H4 at the cdc2 promoter to activate transcription.

  9. Elementos del sistema electoral del Congreso Mexicano

    Directory of Open Access Journals (Sweden)

    Aldo Medina García

    2013-07-01

    Full Text Available El presente artículo es un estudio de los componentes del sistema electoral, diseñado por el legislador mexicano para integrar al parlamento federal. Estudio desarrollado a sabiendas de la importancia que reviste el sistema electoral como un medio que permite a los ciudadanos convertir su voto expresado en las urnas en escaños a favor de determinado candidato o partido. En dicho análisis tomamos en cuenta la evolución constitucional del sistema electoral mexicano, la participación de las fuerzas minoritarias en la integración del Senado y de la Cámara de Diputados, la división que se hace del territorio en circunscripciones electorales, la barrera electoral que se fija a los partidos políticos para acceder a la representación en el Parlamento, la fórmula de asignación que se aplica a la votación obtenida, para finalmente concluir que el sistema electoral mexicano es esencialmente mayoritario aun cuando se vista de proporcional.

  10. El Sistema and American Music Education

    Science.gov (United States)

    Lesniak, Melissa

    2012-01-01

    The brainchild of Jose Antonio Abreu, El Sistema, a music education program for aspiring orchestra musicians launched in Venezuela for students of limited means and now spreading to other parts of the world, has become a subject of interest to music teachers and teacher educators in North America. This article examines a bit of the program's…

  11. Sistema de Reinscripciones Vía Internet

    Directory of Open Access Journals (Sweden)

    Bertha Alicia Góngora Serna

    2005-01-01

    Full Text Available Este Proyecto se enfoca al diseño de una página Web para optimizar el sistema de reinscripciones del Instituto Tecnológico de Aguascalientes (ITA, la cual permite que los alumnos utilicen menos tiempo en el proceso de inscripción, además de facilitar que dicha actividad se pueda realizar desde su hogar. Para la elaboración de este proyecto se requirió de Tecnologías web como HTML, JavaScript y JSP. La información necesaria fue reutilizada de los datos que actualmente genera el sistema de reinscripciones. La investigación que se llevó a cabo para sustentar este proyecto indaga desde la historia del Internet, una explicación breve de las tecnologías Web requeridas, algunos sistemas de reinscripciones de diferentes escuelas de nivel superior del país, así como el funcionamiento básico del sistema actual de reinscripciones del ITA

  12. Elementos del sistema electoral del Congreso Mexicano

    Directory of Open Access Journals (Sweden)

    Aldo Medina García

    2013-08-01

    Full Text Available El presente artículo es un estudio de los componentes del sistema electoral, diseñado por el legislador mexicano para integrar al parlamento federal. Estudio desarrollado a sabiendas de la importancia que reviste el sistema electoral como un medio que permite a los ciudadanos convertir su voto expresado en las urnas en escaños a favor de determinado candidato o partido. En dicho análisis tomamos en cuenta la evolución constitucional del sistema electoral mexicano, la participación de las fuerzas minoritarias en la integración del Senado y de la Cámara de Diputados, la división que se hace del territorio en circunscripciones electorales, la barrera electoral que se fija a los partidos políticos para acceder a la representación en el Parlamento, la fórmula de asignación que se aplica a la votación obtenida, para finalmente concluir que el sistema electoral mexicano es esencialmente mayoritario aun cuando se vista de proporcional.

  13. ENEA presenta sistema ADS per trattamento scorie

    CERN Multimedia

    2002-01-01

    "Arriva il sistema Ads (Accelerator driven system) per il trattamento dei rifiuti radioattivi. Lo studio di fattibilita' per l' innovativa tecnologia, realizzato da un gruppo di lavoro congiunto Enea, Cea, Cern e Ansaldo, e' stato presentato oggi nel corso di un workshop presieduto dal premio Nobel per la fisica, Carlo Rubbia" (1/2 page).

  14. Sistemas ultrasónicos para la irrigación del sistema de conductos radiculares

    Directory of Open Access Journals (Sweden)

    A. García Delgado

    2014-04-01

    Full Text Available La eliminación de los restos de tejido pulpar vital y necrótico y de los microorganismos del sistema de conductos radiculares, es esencial para el éxito en endodoncia. La desinfección del canal radicular mediante la irrigación e instrumentación es el factor más importante en la prevención y tratamiento de la periodontitis apical. Al ser imposible con la instrumentación llegar a todas las áreas del sistema de conductos, la irrigación cobra especial importancia. Por este motivo, en la última década se han desarrollado una serie de sistemas de dispensación y agitación de irrigantes, tales como los ultrasonidos. En concreto, en este artículo de revisión se evalúa la información disponible de los diez últimos años sobre la efectividad de los sistemas ultrasónicos para eliminar bacterias, tejido pulpar, restos de dentina y barrillo dentinario, la capacidad de estos dispositivos para hacer que el irrigante penetre en el sistema de conductos radiculares y la seguridad en su uso. Los resultados de la revisión muestran que los sistemas ultrasónicos son más eficaces en el desbridamiento químico, biológico y físico del sistema de conductos radiculares que los sistemas de irrigación convencional, a la vez que son seguros.

  15. PP2A(Cdc55)'s role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function.

    Science.gov (United States)

    Kerr, Gary W; Wong, Jin Huei; Arumugam, Prakash

    2016-01-01

    PP2A(Cdc55) is a highly conserved serine-threonine protein phosphatase that is involved in diverse cellular processes. In budding yeast, meiotic cells lacking PP2A(Cdc55) activity undergo a premature exit from meiosis I which results in a failure to form bipolar spindles and divide nuclei. This defect is largely due to its role in negatively regulating the Cdc Fourteen Early Anaphase Release (FEAR) pathway. PP2A(Cdc55) prevents nucleolar release of the Cdk (Cyclin-dependent kinase)-antagonising phosphatase Cdc14 by counteracting phosphorylation of the nucleolar protein Net1 by Cdk. CDC55 was identified in a genetic screen for monopolins performed by isolating suppressors of spo11Δ spo12Δ lethality suggesting that Cdc55 might have a role in meiotic chromosome segregation. We investigated this possibility by isolating cdc55 alleles that suppress spo11Δ spo12Δ lethality and show that this suppression is independent of PP2A(Cdc55)'s FEAR function. Although the suppressor mutations in cdc55 affect reductional chromosome segregation in the absence of recombination, they have no effect on chromosome segregation during wild type meiosis. We suggest that Cdc55 is required for reductional chromosome segregation during achiasmate meiosis and this is independent of its FEAR function. PMID:27455870

  16. Structural bases of dimerization of yeast telomere protein Cdc13 and its interaction with the catalytic subunit of DNA polymerase α

    Institute of Scientific and Technical Information of China (English)

    Jia Sun; Neal F Lue; Ming Lei; Yuting Yang; Ke Wan; Ninghui Mao; Tai-Yuan Yu; Yi-Chien Lin; Diane C DeZwaan; Brian C Freeman; Jing-Jer Lin

    2011-01-01

    Budding yeast Cdc13-Stnl-Tenl (CST) complex plays an essential role in telomere protection and maintenance, and has been proposed to be a telomere-specific replication protein A (RPA)-like complex. Previous genetic and structural studies revealed a close resemblance between Stn1-Ten1 and RPA32-RPA14. However, the relationship between Cdc13 and RPA70, the largest subunit of RPA, has remained unclear. Here, we report the crystal structure of the N-terminal OB (oligonucleotide/oligosaccharide binding) fold of Cdc13. Although Cdc13 has an RPA70-like domain organization, the structures of Cdc13 OB folds are significantly different from their counterparts in RPA70, suggesting that they have distinct evolutionary origins. Furthermore, our structural and biochemical analyses revealed unexpected dimerization by the N-terminal OB fold and showed that homodimerization is probably a conserved feature of all Cdc13 proteins. We also uncovered the structural basis of the interaction between the Cdc13 N-terminal OB ' fold and the catalytic subunit of DNA polymerase a (Pol1), and demonstrated a role for Cdc13 dimerization in Pol1 binding. Analysis of the phenotypes of mutants defective in Cdc13 dimerization and Cdc13-Pol1 interaction revealed multiple mechanisms by which dimerization regulates telomere lengths in vivo. Collectively, our findings provide novel insights into the mechanisms and evolution of Cdc13.

  17. Manuel J. Peláez (dir., Diccionario de Canonistas y Eclesiasticistas Europeos y Americanos (Tomo I, AV Akademikerverlag GmbH & Co. KG, EAE, Saarbrücken, 2012, 547 págs.

    Directory of Open Access Journals (Sweden)

    Purificación Alcaide-Pulido

    2013-03-01

    Full Text Available ¿Por qué se otorga escasa atención a los canonistas y eclesiasticistas dentro del Derecho canónico y del Derecho eclesiástico? Esta es la cuestión que surge y el motivo de la aparición de la obra Diccionario de canonistas y eclesiasticistas europeos y americanos. Dicha obra aparece como una necesidad científica emanada de la escasa atención que en el ámbito citado se presta a canonistas y a eclesiasticistas, a través de una recopilación de los pensadores más influyentes en las diversas áreas que comprenden el Derecho Canónico y Eclesiástico, a lo largo del último milenio y, por ende, también de interés para iushistoricistas.

  18. Los TLC de Estados Unidos con países latinoamericanos: un modelo de integración económica “superficial” para el continente americano

    Directory of Open Access Journals (Sweden)

    José Julián Tole

    2013-08-01

    Full Text Available El modelo TLC de Estados Unidos se convierte en un instrumento de integración “ideal” para mejorar sus intereses geopolíticos y geoeconómicos en el continente americano, pues facilita la construcción de peldaños o pequeños bloques que mejorarán su peso en la escena internacional y en algunos sectores del comercio internacional. En tal contexto, el objetivo de este trabajo es analizar el modelo “hecho a la medida” por Estados Unidos para regular y profundizar la liberalización comercial, sin considerar de ninguna manera un mayor grado de integración, con los países latinoamericanos.

  19. Los TLC de Estados Unidos con países latinoamericanos: un modelo de integración económica “superficial” para el continente americano

    Directory of Open Access Journals (Sweden)

    José Julián Tole

    2013-07-01

    Full Text Available El modelo TLC de Estados Unidos se convierte en un instrumento de integración “ideal” para mejorar sus intereses geopolíticos y geoeconómicos en el continente americano, pues facilita la construcción de peldaños o pequeños bloques que mejorarán su peso en la escena internacional y en algunos sectores del comercio internacional. En tal contexto, el objetivo de este trabajo es analizar el modelo “hecho a la medida” por Estados Unidos para regular y profundizar la liberalización comercial, sin considerar de ninguna manera un mayor grado de integración, con los países latinoamericanos.

  20. Sistema de radiació solar via USB

    OpenAIRE

    Villanueva López, José Vicente

    2008-01-01

    L’objectiu d’aquest projecte és implementar un sistema de mesura de la irradiació solar a la Terra basat en una placa solar fotovoltaica. S’ha dissenyat i construït un sistema de mesura de la radiació solar que pot ser útil per aquells que instal·len sistemes fotovoltaics, que pot permetre l’estudi pràctic de les variables energètiques i econòmiques que ens poden indicar quin sistema és mes rentable construir, si un sistema de pannell solar fix, un sistema de pannell solar o...

  1. Sistemas distribuidos: tecnología y casos de estudio

    OpenAIRE

    Pesado, Patricia Mabel; Thomas, Pablo Javier; Bertone, Rodolfo Alfredo; Ramón, Hugo Dionisio; Boracchia, Marcos; Ibañez, Eduardo; Estrebou, César Armando; Marrero, Luciano; Delía, Lisandro; Galdámez, Nicolás; Cáseres, Germán; Albanesi, María Bernarda

    2012-01-01

    Se presenta una línea de investigación y desarrollo, que tiene por objeto estudiar temas relacionados con aspectos de Ingeniería de Software orientados al desarrollo e implementación de proyectos concretos de Sistemas Distribuidos, en particular Sistemas Distribuidos con restricciones de Tiempo Real, Sistemas Web, Sistemas Sensibles al Contexto y Sistemas para Dispositivos Móviles, considerando las extensiones necesarias en las metodologías y herramientas clásicas. Se ha logrado transferir lo...

  2. Lista actualizada y clave ilustrada para los géneros de misidáceos (Crustacea, Peracarida del Mar Intra-Americano Updated checklist and illustrated key to mysid (Crustacea, Peracarida genera from the Intra-American Sea

    Directory of Open Access Journals (Sweden)

    Manuel Ortiz

    2012-12-01

    Full Text Available Se presenta una lista actualizada y una clave ilustrada para la identificación de los 38 géneros marinos, estuarinos y estigobios de los misidáceos que habitan en el Mar Intra-Americano. Estos crustáceos incluyen 6 familias, 5 subfamilias y 5 tribus dentro de los órdenes Lophogastrida y Mysida. El primero contiene 5 géneros y el segundo 33; de éstos, 35 habitan el ambiente marino y el salobre, 2 el cárstico y 1, Antromysis, vive en cualquiera de los 3 ambientes. Los géneros Anchialina, Siriella, Mysidopsis, Mysidium y Heteromysis se distribuyen ampliamente en todo el Mar Intra-Americano. Contrariamente a Chalaraspidum, Euchaetomera, Eucopia, Gironomysis, Metamblyops, Mysidella, Palaumysis, Platymysis, Pleurerythrops, Pseudomma y Synerythrops con una distribución geográfica restringida. El Mar Caribe y el Golfo de México representan las 2 regiones biogeográficas con mayor número de géneros de misidáceos.An updated checklist and an illustrated key for the identification of the 38 genera of marine, estuarine and stygobitic opossum shrimps recorded from the Intra-American Sea, are presented. These crustaceans were included in 6 families, 5 subfamilies, and 5 tribes, into the suborders Lophogastrida and Mysida. First order grouped 5 genera and the second 33. Of these genera, 35 live in marine and estuarine environments, 2 are stygobitic and another, Antromysis, is distributed in each one. The genera Anchialina, Siriella, Mysidopsis, Mysidium and Heteromysis have a wide distribution in overall Intra-American Sea, instead of Chalaraspidum, Euchaetomera, Eucopia, Gironomysis, Metamblyops, Mysidella, Palaumysis, Platymysis, Pleurerythrops, Pseudomma and Synerythrops with a restricted geographical distribution. The Caribbean Sea and the Gulf of Mexico constitute the regions with the biggest number of mysids genera.

  3. Isolation and characterization of new alleles of the cyclin-dependent kinase gene CDC28 with cyclin-specific functional and biochemical defects.

    Science.gov (United States)

    Levine, K; Oehlen, L J; Cross, F R

    1998-01-01

    The G1 cyclin Cln2 negatively regulates the mating-factor pathway. In a genetic screen to identify factors required for this regulation, we identified an allele of CDC28 (cdc28-csr1) that blocked this function of Cln2. Cln2 immunoprecipitated from cdc28-csr1 cells was completely defective in histone H1 kinase activity, due to defects in Cdc28 binding and activation by Cln2. In contrast, Clb2-associated H1 kinase and Cdc28 binding was normal in immunoprecipitates from these cells. cdc28-csr1 was significantly deficient in other aspects of genetic interaction with Cln2. The cdc28-csr1 mutation was determined to be Q188P, in the T loop distal to most of the probable Cdk-cyclin interaction regions. We performed random mutagenesis of CDC28 to identify additional alleles incapable of causing CLN2-dependent mating-factor resistance but capable of complementing cdc28 temperature-sensitive and null alleles. Two such mutants had highly defective Cln2-associated kinase, but, surprisingly, two other mutants had levels of Cln2-associated kinase near to wild-type levels. We performed a complementary screen for CDC28 mutants that could cause efficient Cln2-dependent mating-factor resistance but not complement a cdc28 null allele. Most such mutants were found to alter residues essential for kinase activity; the proteins had little or no associated kinase activity in bulk or in association with Cln2. Several of these mutants also functioned in another assay for CLN2-dependent function not involving the mating-factor pathway, complementing the temperature sensitivity of a cln1 cln3 cdc28-csr1 strain. These results could indicate that Cln2-Cdc28 kinase activity is not directly relevant to some CLN2-mediated functions. Mutants of this sort should be useful in differentiating the function of Cdc28 complexed with different cyclin regulatory subunits. PMID:9418876

  4. Vectorizing the Monte Carlo algorithm for lattice gauge theory calculations on the CDC cyber 205

    Science.gov (United States)

    Barkai, D.; Moriarty, K. J. M.

    1982-06-01

    Lattice gauge theory is a technique for studying quantum field theory free of divergences. All the Monte Carlo computer calculations up to now have been performed on scalar machines. A technique has been developed for effectively vectorizing this class of Monte Carlo problems. The key for vectorizing is in finding groups in finding groups of points on the space-time lattice which are independent of each other. This requires a particular ordering of points along diagonals. A technique for matrix multiply is used which enables one to get the whole of the result matrix in one pass. The CDC CYBER 205 is most suitable for this class of problems using random "index-lists" (arising from the ordering algorithm and the use of random numbers) due to the hardware implementation of "GATHER" and "SCATTER" operations performing at a streaming-rate. A preliminary implementation of this method has executed 5 times faster than on the CDC 7600 system.

  5. Cdc42/N-WASP signaling links actin dynamics to pancreatic beta cell delamination and differentiation

    DEFF Research Database (Denmark)

    Kesavan, Gokul; Lieven, Oliver; Mamidi, Anant;

    2014-01-01

    Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination and epithelial cell movement within the plane of an epithelium are associated with a change in cellular phenotype. However, how this positional change is linked to differentiat......Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination and epithelial cell movement within the plane of an epithelium are associated with a change in cellular phenotype. However, how this positional change is linked...... to differentiation remains unknown. Using the developing mouse pancreas as a model system, we show that β cell delamination and differentiation are two independent events, which are controlled by Cdc42/N-WASP signaling. Specifically, we show that expression of constitutively active Cdc42 in β cells inhibits β cell...

  6. A Review of the CDC Recommendations for Prevention of HAIs in Outpatient Settings.

    Science.gov (United States)

    Garrett, J Hudson

    2015-05-01

    According to the Centers for Disease Control and Prevention (CDC), most health care-associated infections (HAIs) are caused by contamination from the hands of health care providers or patients, contamination from the environment, and contamination from the patient's own skin. To mitigate common sources of infection transmission, frontline health care providers must be compliant with basic infection-prevention interventions, including hand hygiene, environmental cleaning and disinfection, safe injection practices, and designation of a trained health care professional to be responsible for the infection prevention and control program. Integration of CDC recommendations should incorporate a bundled approach to these interventions and should be part of a comprehensive approach to infection prevention and control. Effective infection-prevention practices in outpatient settings are critical for reducing the risk of infection transmission, improving patient safety and patient outcomes, and reducing costs associated with health care delivery.

  7. A Decision Processing Algorithm for CDC Location Under Minimum Cost SCM Network

    Science.gov (United States)

    Park, N. K.; Kim, J. Y.; Choi, W. Y.; Tian, Z. M.; Kim, D. J.

    Location of CDC in the matter of network on Supply Chain is becoming on the high concern these days. Present status of methods on CDC has been mainly based on the calculation manually by the spread sheet to achieve the goal of minimum logistics cost. This study is focused on the development of new processing algorithm to overcome the limit of present methods, and examination of the propriety of this algorithm by case study. The algorithm suggested by this study is based on the principle of optimization on the directive GRAPH of SCM model and suggest the algorithm utilizing the traditionally introduced MST, shortest paths finding methods, etc. By the aftermath of this study, it helps to assess suitability of the present on-going SCM network and could be the criterion on the decision-making process for the optimal SCM network building-up for the demand prospect in the future.

  8. 75 FR 4830 - Advisory Committee to the Director (ACD), Centers for Disease Control (CDC) and Prevention-Ethics...

    Science.gov (United States)

    2010-01-29

    ...), Centers for Disease Control (CDC) and Prevention--Ethics Subcommittee (ES) In accordance with section 10(a... health ethics questions and issues arising from programs, scientists, and practitioners. Matter to...

  9. 75 FR 7483 - Advisory Committee to the Director (ACD), Centers for Disease Control (CDC) and Prevention-Ethics...

    Science.gov (United States)

    2010-02-19

    ... February 18, 2010, meeting published at 75 FR 4830, January 29, 2010, is 1 p.m.-5 p.m. FOR FURTHER...), Centers for Disease Control (CDC) and Prevention--Ethics Subcommittee (ES); Correction AGENCY: Centers...

  10. 78 FR 64221 - CDC/HRSA Advisory Committee on HIV, Viral Hepatitis and STD Prevention and Treatment; Notice of...

    Science.gov (United States)

    2013-10-28

    ... Hepatitis and STD Prevention and Treatment; Notice of Meeting In accordance with section l0(a)(2) of the... meeting. Name: CDC/HRSA Advisory Committee on HIV, Viral Hepatitis and STD Prevention and Treatment...

  11. 77 FR 66469 - CDC/HRSA Advisory Committee on HIV, Viral Hepatitis and STD Prevention and Treatment

    Science.gov (United States)

    2012-11-05

    ... Hepatitis and STD Prevention and Treatment In accordance with section 10(a)(2) of the Federal Advisory... Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, CDC, 1600 Clifton Road NE., Mailstop...

  12. Simpósio Ibero-Americano de História da Enfermagem: novas perspectivas da produção intelectual em história da enfermagem Coloquio Ibero-Americano de Historia de la Enfermería: nuevas perspectivas de la producción intelectual en historia de la enfermería Ibero-American Symposium on History of Nursing: new perspectives of intellectual production in the history of nursing

    OpenAIRE

    Almerinda Moreira; Fernando Porto; Genival Fernandes de Freitas; Paulo Fernando de Souza Campos

    2009-01-01

    A produção intelectual apresentada no I Simpósio Ibero-Americano de História da Enfermagem foi organizada a partir de três eixos temáticos: antropologia do cuidado, história das instituições de ensino, de assistência e agremiações de enfermagem e história de vida, totalizando 198 estudos. Mediante os resultados apresentados a produção mostrada no simpósio apontou para a tendência dos estudos em história da enfermagem, nos eixos temáticos, pela ordem de interesse dos pesquisadores em: história...

  13. Activated Cdc42-associated kinase Ack1 promotes prostate cancer progression via androgen receptor tyrosine phosphorylation

    OpenAIRE

    Mahajan, Nupam P.; Liu, Yuanbo; Majumder, Samarpan; Warren, Maria R.; Parker, Carol E.; Mohler, James L.; Earp, H. Shelton; Whang, Young E.

    2007-01-01

    Activation of the androgen receptor (AR) may play a role in androgen-independent progression of prostate cancer. Multiple mechanisms of AR activation, including stimulation by tyrosine kinases, have been postulated. We and others have recently shown involvement of activated Cdc42-associated tyrosine kinase Ack1 in advanced human prostate cancer. Here we provide the molecular basis for interplay between Ack1 and AR in prostate cancer cells. Activated Ack1 promoted androgen-independent growth o...

  14. Cdc42p and Fus2p act together late in yeast cell fusion

    OpenAIRE

    Ydenberg, Casey A.; Stein, Richard A; Rose, Mark D.

    2012-01-01

    Cell fusion is the key event of fertilization that gives rise to the diploid zygote and is a nearly universal aspect of eukaryotic biology. In the yeast Saccharomyces cerevisiae, several mutants have been identified that are defective for cell fusion, and yet the molecular mechanism of this process remains obscure. One obstacle has been that genetic screens have mainly focused on mating-specific factors, whereas the process likely involves housekeeping proteins as well. Here we implicate Cdc4...

  15. CDC Vital Signs–Dental Sealants Prevent Cavities

    Centers for Disease Control (CDC) Podcasts

    2016-10-18

    This podcast is based on the October 2016 CDC Vital Signs report. Dental sealants, applied soon after a child's permanent molars come in, can protect against cavities for up to nine years. Applying sealants in schools for low-income children could save millions in dental treatment costs.  Created: 10/18/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/18/2016.

  16. CDC Vital Signs–Motor Vehicle Crash Deaths

    Centers for Disease Control (CDC) Podcasts

    2016-07-06

    This podcast is based on the July 2016 CDC Vital Signs report. In the U.S., about 90 people die in motor vehicle crashes each day and thousands more are injured, resulting in hundreds of millions of dollars in direct medical costs each year. Learn what you can do to stay safe.  Created: 7/6/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 7/6/2016.

  17. CDC Signos Vitales: Piense en la septicemia. El tiempo es crucial. (Think Sepsis. Time Matters.)

    Centers for Disease Control (CDC) Podcasts

    2016-08-23

    Este podcast se basa en la edición de agosto del 2016 del informe Signos Vitales de los CDC. La septicemia es una emergencia médica y puede ocurrir rápidamente. Conozca los signos de la septicemia y la forma de prevenirla.  Created: 8/23/2016 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 8/23/2016.

  18. CDC Vital Signs-Zika and Pregnancy: What You Should Know

    Centers for Disease Control (CDC) Podcasts

    2016-04-01

    This podcast is based on the April 2016 CDC Vital Signs report. A pregnant woman who is infected with Zika virus can pass it to her fetus which is linked to microcephaly, a serious birth defect. This podcast discusses how to protect yourself from Zika virus.  Created: 4/1/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 4/1/2016.

  19. CDC Vital Signs-ADHD in Young Children: What You Should Know

    Centers for Disease Control (CDC) Podcasts

    2016-05-03

    This podcast is based on the May 2016 CDC Vital Signs report. For children ages two to five who have ADHD, behavior therapy is recommended before prescribing medicine. This therapy teaches parents ways to improve their child’s behavior and can work as well as medicine, without the risk of side effects.  Created: 5/3/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 5/3/2016.

  20. Human infections caused by Brevibacterium casei, formerly CDC groups B-1 and B-3.

    OpenAIRE

    Gruner, E; Steigerwalt, A G; Hollis, D G; Weyant, R S; Weaver, R E; Moss, C W; M Daneshvar; J. M. Brown; Brenner, D J

    1994-01-01

    Forty-one clinical strains of CDC coryneform groups B-1 and B-3 were compared biochemically, by analysis of cell wall sugars, amino acids, and cellular fatty acids, and by DNA relatedness to the type strains of Brevibacterium casei, Brevibacterium epidermidis, and Brevibacterium linens. Twenty-two strains were shown to be B. casei, while five other strains formed a phenotypically inseparable genomospecies in the same genus. The remaining isolates were genetically heterogeneous, and most are p...

  1. Microbiological characteristics of Weeksella virosa (formerly CDC group IIf) isolated from the human genitourinary tract.

    OpenAIRE

    Reina, J; Gil, J.; Salva, F; J. Gomez; Alomar, P

    1990-01-01

    Weeksella virosa (formerly CDC group IIf) is a nonsaccharolytic, oxidase- and catalase-positive, gram-negative rod which is unable to grow on MacConkey agar. At 48 h of incubation on blood or chocolate agar, the colonies present a characteristic appearance: intensely mucoid, adherent, and cream colored as a result of the production of a nondiffusible yellow pigment. This microorganism has been isolated predominantly from the female genitourinary tract, which indicates the opportunity for sexu...

  2. Cdc7-Dbf4 Is a Gene-Specific Regulator of Meiotic Transcription in Yeast

    OpenAIRE

    Lo, Hsiao-Chi; Kunz, Ryan C.; Chen, Xiangyu; Marullo, Allison; Steven P Gygi; Hollingsworth, Nancy M.

    2012-01-01

    Meiosis divides the chromosome number of the cell in half by having two rounds of chromosome segregation follow a single round of chromosome duplication. The first meiotic division is unique in that homologous pairs of sister chromatids segregate to opposite poles. Recent work in budding and fission yeast has shown that the cell cycle kinase, Cdc7-Dbf4, is required for many meiosis-specific chromosomal functions necessary for proper disjunction at meiosis I. This work reveals another role for...

  3. Congenital syphilis after treatment of maternal syphilis with a penicillin regimen exceeding CDC guidelines.

    OpenAIRE

    Conover, C S; Rend, C A; Miller, G B; Schmid, G P

    1998-01-01

    BACKGROUND: Although congenital syphilis usually occurs as a result of a failure to detect and treat syphilis in pregnant women, failures of the currently recommended regimen to prevent congenital syphilis have been reported. CASE: This report describes an infant with congenital syphilis despite maternal treatment with a regimen exceeding current CDC guidelines. CONCLUSION: Regardless of the regimen used to treat syphilis during pregnancy, clinicians should recognize the possibility of occasi...

  4. Single site suppressors of a fission yeast temperature-sensitive mutant in cdc48 identified by whole genome sequencing.

    Directory of Open Access Journals (Sweden)

    Irina N Marinova

    Full Text Available The protein called p97 in mammals and Cdc48 in budding and fission yeast is a homo-hexameric, ring-shaped, ubiquitin-dependent ATPase complex involved in a range of cellular functions, including protein degradation, vesicle fusion, DNA repair, and cell division. The cdc48+ gene is essential for viability in fission yeast, and point mutations in the human orthologue have been linked to disease. To analyze the function of p97/Cdc48 further, we performed a screen for cold-sensitive suppressors of the temperature-sensitive cdc48-353 fission yeast strain. In total, 29 independent pseudo revertants that had lost the temperature-sensitive growth defect of the cdc48-353 strain were isolated. Of these, 28 had instead acquired a cold-sensitive phenotype. Since the suppressors were all spontaneous mutants, and not the result of mutagenesis induced by chemicals or UV irradiation, we reasoned that the genome sequences of the 29 independent cdc48-353 suppressors were most likely identical with the exception of the acquired suppressor mutations. This prompted us to test if a whole genome sequencing approach would allow us to map the mutations. Indeed genome sequencing unambiguously revealed that the cold-sensitive suppressors were all second site intragenic cdc48 mutants. Projecting these onto the Cdc48 structure revealed that while the original temperature-sensitive G338D mutation is positioned near the central pore in the hexameric ring, the suppressor mutations locate to subunit-subunit and inter-domain boundaries. This suggests that Cdc48-353 is structurally compromized at the restrictive temperature, but re-established in the suppressor mutants. The last suppressor was an extragenic frame shift mutation in the ufd1 gene, which encodes a known Cdc48 co-factor. In conclusion, we show, using a novel whole genome sequencing approach, that Cdc48-353 is structurally compromized at the restrictive temperature, but stabilized in the suppressors.

  5. Waist Circumferences of Chilean Students: Comparison of the CDC-2012 Standard and Proposed Percentile Curves

    Directory of Open Access Journals (Sweden)

    Rossana Gómez-Campos

    2015-07-01

    Full Text Available The measurement of waist circumference (WC is considered to be an important means to control overweight and obesity in children and adolescents. The objectives of the study were to (a compare the WC measurements of Chilean students with the international CDC-2012 standard and other international standards, and (b propose a specific measurement value for the WC of Chilean students based on age and sex. A total of 3892 students (6 to 18 years old were assessed. Weight, height, body mass index (BMI, and WC were measured. WC was compared with the CDC-2012 international standard. Percentiles were constructed based on the LMS method. Chilean males had a greater WC during infancy. Subsequently, in late adolescence, males showed values lower than those of the international standards. Chilean females demonstrated values similar to the standards until the age of 12. Subsequently, females showed lower values. The 85th and 95th percentiles were adopted as cutoff points for evaluating overweight and obesity based on age and sex. The WC of Chilean students differs from the CDC-2012 curves. The regional norms proposed are a means to identify children and adolescents with a high risk of suffering from overweight and obesity disorders.

  6. P-cadherin promotes collective cell migration via a Cdc42-mediated increase in mechanical forces

    Science.gov (United States)

    Plutoni, Cédric; Bazellieres, Elsa; Le Borgne-Rochet, Maïlys; Comunale, Franck; Brugues, Agusti; Séveno, Martial; Planchon, Damien; Thuault, Sylvie; Morin, Nathalie; Bodin, Stéphane; Trepat, Xavier

    2016-01-01

    Collective cell migration (CCM) is essential for organism development, wound healing, and metastatic transition, the primary cause of cancer-related death, and it involves cell–cell adhesion molecules of the cadherin family. Increased P-cadherin expression levels are correlated with tumor aggressiveness in carcinoma and aggressive sarcoma; however, how P-cadherin promotes tumor malignancy remains unknown. Here, using integrated cell biology and biophysical approaches, we determined that P-cadherin specifically induces polarization and CCM through an increase in the strength and anisotropy of mechanical forces. We show that this mechanical regulation is mediated by the P-cadherin/β-PIX/Cdc42 axis; P-cadherin specifically activates Cdc42 through β-PIX, which is specifically recruited at cell–cell contacts upon CCM. This mechanism of cell polarization and migration is absent in cells expressing E- or R-cadherin. Thus, we identify a specific role of P-cadherin through β-PIX–mediated Cdc42 activation in the regulation of cell polarity and force anisotropy that drives CCM. PMID:26783302

  7. NRP1 Regulates CDC42 Activation to Promote Filopodia Formation in Endothelial Tip Cells

    Directory of Open Access Journals (Sweden)

    Alessandro Fantin

    2015-06-01

    Full Text Available Sprouting blood vessels are led by filopodia-studded endothelial tip cells that respond to angiogenic signals. Mosaic lineage tracing previously revealed that NRP1 is essential for tip cell function, although its mechanistic role in tip cells remains poorly defined. Here, we show that NRP1 is dispensable for genetic tip cell identity. Instead, we find that NRP1 is essential to form the filopodial bursts that distinguish tip cells morphologically from neighboring stalk cells, because it enables the extracellular matrix (ECM-induced activation of CDC42, a key regulator of filopodia formation. Accordingly, NRP1 knockdown and pharmacological CDC42 inhibition similarly impaired filopodia formation in vitro and in developing zebrafish in vivo. During mouse retinal angiogenesis, CDC42 inhibition impaired tip cell and vascular network formation, causing defects that resembled those due to loss of ECM-induced, but not VEGF-induced, NRP1 signaling. We conclude that NRP1 enables ECM-induced filopodia formation for tip cell function during sprouting angiogenesis.

  8. GIT1 promotes lung cancer cell metastasis through modulating Rac1/Cdc42 activity and is associated with poor prognosis

    OpenAIRE

    Chang, Jeng-Shou; Su, Chia-Yi; Yu, Wen-Hsuan; Lee, Wei-Jiunn; Liu, Yu-Peng; Lai, Tsung-Ching; Jan, Yi-Hua; Yang, Yi-Fang; Shen, Chia-Ning; Shew, Jin-Yuh; Lu, Jean; Yang, Chih-Jen; Huang, Ming-Shyan; Lu, Pei-Jung; Lin, Yuan-Feng

    2015-01-01

    G-protein-coupled receptor kinase interacting protein 1 (GIT1) is participated in cell movement activation, which is a fundamental process during tissue development and cancer progression. GIT1/PIX forming a functional protein complex that contributes to Rac1/Cdc42 activation, resulting in increasing cell mobility. Although the importance of Rac1/Cdc42 activation is well documented in cancer aggressiveness, the clinical importance of GIT1 remains largely unknown. Here, we investigated the cli...

  9. Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics*

    OpenAIRE

    Gnad, Florian; Young, Amy; Zhou, Wei; Lyle, Karen; Ong, Christy C.; Matthew P. Stokes; Silva, Jeffrey C.; Belvin, Marcia; Friedman, Lori S.; Koeppen, Hartmut; Minden, Audrey; Hoeflich, Klaus P.

    2013-01-01

    Although K-Ras, Cdc42, and PAK4 signaling are commonly deregulated in cancer, only a few studies have sought to comprehensively examine the spectrum of phosphorylation-mediated signaling downstream of each of these key signaling nodes. In this study, we completed a label-free quantitative analysis of oncogenic K-Ras, activated Cdc42, and PAK4-mediated phosphorylation signaling, and report relative quantitation of 2152 phosphorylated peptides on 1062 proteins. We define the overlap in phosphop...

  10. Correlation between δ-catenin and Cdc42 expression in non-small cell lung cancer%δ-catenin与Cdc42在非小细胞肺癌中表达的相关性

    Institute of Scientific and Technical Information of China (English)

    张迪; 王恩华

    2016-01-01

    背景与目的:δ-catenin是p120 catenin亚家族中的成员,可与细胞膜上的E-cadherin直接结合,形成E-cadherin/catenin复合体。δ-catenin还可以通过调节Cdc42(Small GTP酶)活性以影响细胞骨架装配。该研究检测非小细胞肺癌(non-small cell lung cancer,NSCLC)中δ-catenin及Cdc42的表达情况并探讨了二者表达的相关性。方法:采用免疫组织化学方法检测122例NSCLC标本中δ-catenin与Cdc42的表达。采用蛋白[质]印迹法(Western blot)及逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)法检测肺癌组织中δ-catenin及Cdc42的蛋白及mRNA表达情况。在肺癌细胞系中,分别上调或干扰δ-catenin的表达后,利用G-LISA及Transwell小室法检测Cdc42活性以及肺癌细胞侵袭能力的改变。结果:δ-catenin和Cdc42在肺癌组织中其蛋白及mRNA表达明显高于正常肺组织。而在122例NSCLC病例中,δ-catenin阳性表达率为65.57%(80/122),Cdc42过表达率为68.03%(83/122)。δ-catenin阳性表达和Cdc42的过表达具有较好的相关性(P<0.001)。δ-catenin和Cdc42的协同表达与肺癌的高临床分期、低分化、病理类型为腺癌和淋巴结转移相关(P<0.05),并且与肺癌患者的不良预后明显相关。在肺癌细胞系中通过调节δ-catenin表达,改变Cdc42的表达及活性,影响肺癌细胞的侵袭能力。结论:在肺癌组织中δ-catenin和Cdc42的表达具有相关性,而二者协同表达与患者不良预后相关。%Background and purpose:δ-catenin is a member of the p120 catenin subfamily, which can directly bind to E-cadherin on the cell membrane, forming E-cadherin/catenin complex. δ-catenin can also affect the cytoskeleton assembly by regulating the activity of Cdc42 (Small GTPase). Therefore, this study detected the expression of δ-catenin and Cdc42 in non-small cell lung cancer (NSCLC) and investigated the relationship between them

  11. CDC25A Protein Stability Represents a Previously Unrecognized Target of HER2 Signaling in Human Breast Cancer: Implication for a Potential Clinical Relevance in Trastuzumab Treatment

    Directory of Open Access Journals (Sweden)

    Emanuela Brunetto

    2013-06-01

    Full Text Available The CDC25A-CDK2 pathway has been proposed as critical for the oncogenic action of human epidermal growth factor receptor 2 (HER2 in mammary epithelial cells. In particular, transgenic expression of CDC25A cooperates with HER2 in promoting mammary tumors, whereas CDC25A hemizygous loss attenuates the HER2-induced tumorigenesis penetrance. On the basis of this evidence of a synergism between HER2 and the cell cycle regulator CDC25A in a mouse model of mammary tumorigenesis, we investigated the role of CDC25A in human HER2-positive breast cancer and its possible implications in therapeutic response. HER2 status and CDC25A expression were assessed in 313 breast cancer patients and we found statistically significant correlation between HER2 and CDC25A (P = .007. Moreover, an HER2-positive breast cancer subgroup with high levels of CDC25A and very aggressive phenotype was identified (P = .005. Importantly, our in vitro studies on breast cancer cell lines showed that the HER2 inhibitor efficacy on cell growth and viability relied also on CDC25A expression and that such inhibition induces CDC25A down-regulation through phosphatidylinositol 3-kinase/protein kinase B pathway and DNA damage response activation. In line with this observation, we found a statistical significant association between CDC25A overexpression and trastuzumab-combined therapy response rate in two different HER2-positive cohorts of trastuzumab-treated patients in either metastatic or neoadjuvant setting (P = .018 for the metastatic cohort and P = .021 for the neoadjuvant cohort. Our findings highlight a link between HER2 and CDC25A that positively modulates HER2- targeted therapy response, suggesting that, in HER2-positive breast cancer patients, CDC25A overexpression affects trastuzumab sensitivity.

  12. La decadencia del Sistema Nacional de Salud

    Directory of Open Access Journals (Sweden)

    Costas Lombardía, Enrique

    2005-02-01

    Full Text Available El Sistema Nacional de Salud no es, como oficialmente se enseña, un todo ramificado o descentralizado, sino un mosaico compuesto por 17 «todos », los 17 servicios sanitarios autonómicos, cada uno de ellos con poder estatutario para configurarse a sí mismo como entidad propia, sin interferencias de la administración central. Servicios casi soberanos, diferentes en criterios de asistencia y pautas administrativas, refractarios a su coordinación y cohesión, y hasta desconocidos entre si. Cada uno responde sólo ante los ciudadanos votantes de su comunidad, y está ensimismado en sus ambiciones (su política, sus posibles votos en busca del provecho partidista propio, y desinteresado del Sistema Nacional…

  13. Sistemas electorales y justicia electoral a debate

    Directory of Open Access Journals (Sweden)

    Dieter Nohlen

    2015-07-01

    Full Text Available Despliega conceptos y planteamientos en torno a los sistemas electorales y la justicia electoral. El autor inicia con una descripción estricta de los sistemas electorales para orientar, en una segunda parte, un debate sobre la justicia electoral, que marca dos tendencias: la judicialización de la política y la politización de la administración y la jurisdicción electoral. Concluye que el desarrollo de la justicia electoral en América Latina, en sus múltiples dimensiones, es comprensible sólo con referencia al contexto sociocultural y político de la región

  14. Energia no Sistema Climático

    OpenAIRE

    Andrade, José

    2011-01-01

    AS FORMAS DE ENERGIA DO SISTEMA CLIMÁTICO. MODOS DE TRANSFERÊNCIA DE ENERGIA.A RADIAÇÃO SOLAR, TERRESTRE E ATMOSFÉRICA (características da radiação,Radiação Solar, Radiação terrestre e radiação atmosférica…. BALANÇO ENERGÉTICO DO SISTEMA GLOBO-ATMOSFERA.BALANÇO DE RADIAÇÃO DE UMA SUPERFÍCIE (Componentes do balanço de radiação,Medição e estimativa dos componentes do balanço de radiação).BALANÇO ENERGÉTICO À SUPERFÍCIE TERRESTRE.CONSEQUÊNCIAS NO MOVIMENTO DO AR ATMOSFÉRICO.EXERCÍCIOS PROPOSTOS....

  15. El vulcanismo en el Sistema Solar

    OpenAIRE

    Anguita, Francisco

    1999-01-01

    El vulcanismo es el proceso geológico más común en el Sistema Solar. En la Tierra y en Marte, los magmas más frecuentes son el basáltico y el andesítico; en general, los basaltos parecen ser la roca volcánica predominante en todo el interior del Sistema Solar, mientras que en el exterior abundan los magmas de volátiles, sobre todo agua. Tras una rápida revisión del vulcanismo en los principales cuerpos planetarios, se analiza su marco dinámico: mientras que en la Tierra hay magmas en borde...

  16. CATEGORIZANDO A LOS USUARIOS DE SISTEMAS DIGITALES.

    Directory of Open Access Journals (Sweden)

    Denise Hernández y Hernández

    2014-01-01

    Full Text Available Repasamos las categorías más utilizadas para aludir a las características de las personas que utilizan algún tipo de sistema digital, ya sea por su grado de participación en la comu- nicación global y en los sistemas web -1.0 o 2.0-; por la actitud que toman ante el contenido digital -consumidores o productores-; por su intermitencia en el mundo digital -residentes o visitantes-; o por el momento histórico en el que nacieron -nativos digitales o inmigrantes digitales-, con el propósito de reflexionar sobre los cambios y las consecuencias que han impulsado los recursos y las herramientas digitales.

  17. GPS: El sistema de posicionamiento global

    Directory of Open Access Journals (Sweden)

    Juan Gilberto Serpas

    2016-03-01

    Full Text Available El Sistema de Posicionamiento Global (GPS, por sus siglas en inglés se ha convertido, en la actualidad, en una herramienta invaluable para el posicionamiento de puntos sobre la superficie terrestre. Este artículo pretende dar al lector una descripción del GPS, así como la introducción al cálculo de coordenadas para ser usadas tanto en navegación como en labores de topografía y geodesia. Las características principales del sistema son descritas y se introducen los principios básicos para la determinación de coordenadas tanto en modo absoluto como en modo relativo.

  18. El Sistema Político Latinoamericano

    Directory of Open Access Journals (Sweden)

    Luis Dallanegra Pedraza

    2003-01-01

    Full Text Available Se ha escrito mucho sobre la problemática latinoamericana, y sobre sus aspectos históricos, especialmente en relación con Estados Unidos, y sobre las políticas que este país ha ido aplicando históricamente en sus sucesivos períodos de gobierno, hacia América Latina. Sin embargo, no se ha hecho un estudio profundo sobre el sistema político latinoamericano. Al menos sobre la existencia de un sistema político específico en América Latina que la caracterice desde ese punto de vista. Siempre se han utilizado las tipologías políticas europeas para caracterizar al continente suramericano, siendo que se trata de sociedades y culturas políticas diferentes. Es cierto que existe una “geografía” llamada América Latina; lo que no necesariamente coincide con la existencia de un sistema político que abarque esa región. Lo más peculiar es que, lo que era considerado como la “verdad” en la América Latina de los cuarentas, cincuentas, sesentas, e incluso hasta los ochentas; luego de la caída del Muro de Berlín en 1989 y la desintegración de la URSS en 1991, paso a ser lo errado. Se procura mostrar el hilo conductor del comportamiento de los sistemas políticos latinoamericanos.

  19. Vpliv vetrnih elektrarn na stabilnost elektroenergetskega sistema

    OpenAIRE

    GYOEFI, TOMI

    2014-01-01

    V diplomskem delu so, na podlagi literature, analizirane dinamične lastnosti različnih tipov vetrnih elektrarn v elektroenergetskem sistemu. Namen dela je ponuditi pregled generacije vetrne energije, pri katerem je poudarek na vplivu vetrnih elektrarn na stabilnost elektroenergetskega sistema. Podani so osnovni mehanizmi delovanja asinhronskega generatorja, dvojno napajanega asinhronskega generatorja in sinhronskega generatorja s pretvornikom, njihovo tranzientno obratovanje in zmožnost...

  20. usando dinámica de sistemas

    Directory of Open Access Journals (Sweden)

    Carlos Mario Parra Mesa

    2006-01-01

    Full Text Available Este artículo pretende motivar a los estamentos académicos y a las pequeñas y medianas organizaciones a desarrollar en el área de la simulación dinámica de sistemas trabajos conjuntos que conlleven a un direccionamiento empresarial más claro y confiable, y de paso contribuyan a la formación de profesionales mejor capacitados para satisfacer las necesidades del medio. Para ello se presenta la aplicación de la simulación dinámica de sistemas en una pequeña empresa, mediante la modelación y simulación computacional del proceso de plastificado de barra metálica para carpetas de FRAMECO S.A (Bello-Antioquia, con interés particular en la identificación de las limitaciones del sistema, la capacidad de producción, el tiempo tipo del proceso, el tiempo ocioso de la mano de obra y el tiempo de consumo innecesario del gas empleado como insumo en uno de los equipos; igualmente, se ilustra de manera general el análisis de sensibilidad del proceso. Como resultado se obtiene un modelo de simulación que representa de manera razonable el comportamiento dinámico del proceso y le facilita al directivo, académico o analista, la comprensión, el análisis y el mejoramiento del sistema productivo.

  1. Variabilidade espacial da produtividade do feijoeiro correlacionada com atributos químicos de um Latossolo Vermelho Distroférrico sob sistema de semeadura direta

    Directory of Open Access Journals (Sweden)

    Flávio Carlos Dalchiavon

    2011-01-01

    Full Text Available O feijão, importante fonte proteica, é um alimento muito comum na dieta da população dos países latino-americanos, sendo o Brasil maior produtor mundial deste legume. Nacionalmente, seu cultivo no sistema de semeadura direta tem crescido ano após ano. Em 2002, foi avaliada a variabilidade espacial do feijoeiro, em função dos atributos químicos de um Latossolo Vermelho Distroférrico no sistema de semeadura direta, em Selvíria (MS no Cerrado Brasileiro. O objetivo do presente estudo foi selecionar, entre os atributos do solo pesquisado, aquele com a melhor correlação, linear e espacial, para explicar a variabilidade da produtividade do feijoeiro. Foi instalada uma rede amostral, para a coleta de dados do solo e das plantas, com 135 pontos amostrais, em uma área de 7500 m². A produtividade de grãos de feijão (PG representou o atributo da planta, enquanto os do solo foram: P, MO, pH, K, Ca, Mg, H+Al, S, T e o V%. Estabeleceram-se correlações lineares, simples e múltiplas, entre a PG e os atributos do solo. Foram modelados semivariogramas para todos os atributos, obtendo-se as respectivas krigagens e validações cruzadas. Também foram estabelecidas as co-krigagens entre a PG e os atributos do solo. Em relação à produtividade de grãos de feijão, 22% da sua variação foram atribuídos à variação nos atributos químicos do solo. Tanto linear quanto espacialmente, o pH do solo foi bom indicador da produtividade de grãos de feijão quando cultivado sob sistema de semeadura direta.

  2. Sistemas grandes de números o sistemas de números grandes

    OpenAIRE

    Oostra, Arnold

    2008-01-01

    Esta es una reflexión sobre la pregunta: ¿Existen conjuntos de números con carnalidad mayor que la de los reales? Los sistemas numéricos más empleados en la matemática se agrupan en tres clases cuando se los observa desde el punto de vista cardinal: los finitos, los que tienen el cardinal de los naturales y los que tienen el de los reales. ¿Existen sistemas numéricos más grandes? Por supuesto, primero debería precisarse qué puede entenderse por “sistema numérico”, pero aquí no se ahondará en ...

  3. Control integral systems; Sistemas integrales de control

    Energy Technology Data Exchange (ETDEWEB)

    Burgos, Estrella [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1998-12-31

    Almost two third of the electric power generation in Mexico are obtained from hydrocarbons, for that reasons Comision Federal de Electricidad (CFE) dedicated special commitment in modernizing the operation of fossil fuel central stations. In attaining this objective the control systems play a fundamental roll, from them depend a good share of the reliability and the efficiency of the electric power generation process, as well as the extension of the equipment useful life. Since 1984 the Instituto de Investigaciones Electricas (IIE) has been working, upon the request of CFE, on the development of digital control systems. To date it has designed and implemented a logic control system for gas burners, which controls 32 burners of the Unit 4 boiler of the Generation Central of Valle de Mexico and two systems for distributed control for two combined cycle central stations, which are: Dos Bocas, Veracruz Combined cycle central, and Gomez Palacio, Durango combined cycle central. With these two developments the IIE enters the World tendency of implementing distributed control systems for the fossil fuel power central update [Espanol] Casi las dos terceras partes de la generacion electrica en Mexico se obtienen a partir de hidrocarburos, es por eso que la Comision Federal de Electricidad (CFE) puso especial empeno en modernizar la operacion de las centrales termoelectricas de combustibles fosiles. En el logro de este objetivo los sistemas de control desempenan un papel fundamental, de ellos depende una buena parte la confiabilidad y la eficiencia en el proceso de generacion de energia electrica, asi como la prolongacion de la vida util de los equipos. Desde 1984 el Instituto de Investigaciones Electricas (IIE) ha trabajado, a solicitud de la CFE, en el desarrollo de sistemas digitales de control. A la fecha se han disenado e implantado un sistema de control logico de quemadores de gas, el cual controla 32 quemadores de la caldera de la unidad 4 de la central de generacion

  4. Isolation of a cdc28 mutation that abrogates the dependence of S phase on completion of M phase of the budding yeast cell cycle

    Indian Academy of Sciences (India)

    Santanu Kumar Ghosh; Pratima Sinha

    2000-01-01

    We have isolated a mutation in the budding yeast Saccharomyces cerevisisae CDC28 gene that allows cdc13 cells, carrying damaged DNA, to continue with the cell division cycle. While cdc13 mutant cells are arrested as large-budded cells at the nonpermissive temperature 37°C, the cdc13 cdc28 double mutant culture showed cells with one or more buds, most of which showed apical growth. The additional buds emerged without the intervening steps of nuclear division and cell separation. We suggest that the cdc28 mutation abrogates a checkpoint function and allows cells with damaged or incompletely replicated DNA an entry to another round of cell cycle and bypasses the mitotic phase of the cell cycle.

  5. Sistemas ultrasónicos para la irrigación del sistema de conductos radiculares

    OpenAIRE

    A. García Delgado; J. Martín-González; L. Castellanos-Cosano; Martín Jiménez, M.; J.J. Segura-Egea

    2014-01-01

    La eliminación de los restos de tejido pulpar vital y necrótico y de los microorganismos del sistema de conductos radiculares, es esencial para el éxito en endodoncia. La desinfección del canal radicular mediante la irrigación e instrumentación es el factor más importante en la prevención y tratamiento de la periodontitis apical. Al ser imposible con la instrumentación llegar a todas las áreas del sistema de conductos, la irrigación cobra especial importancia. Por este motivo, en la última dé...

  6. 猪带绦虫CDC37基因的克隆、表达与组织定位研究%Prokaryotic Expression and Histolocalization of the Taenia solium CDC37 Gene

    Institute of Scientific and Technical Information of China (English)

    黄江; 李波; 戴佳琳; 张爱华

    2013-01-01

    Objective To express Taenia solium gene encoding cell division cycle 37 protein (TsCDC37) and investigate its antigenicity and localization in adults of Taenia solium. Methods The complete coding sequence of TsCDC37 was amplified by PCR based on the recombinant plasmid clone from the cDNA library of adult Taenia solium. The PCR product was cloned into a prokaryotic expression vector pET-28a(+). The recombinant expression plasmid was identified by PCR, double endonuclease digestion and sequencing. The recombinant plasmid was transformed into E. coli BL21/DE3 and followed by expression of the protein induced by IPTG. The mice were immunized subcutaneously with purified reeombinant TsCDC37 formulated in Freund's adjuvant. The antigenicity of the reeombinant protein was examined by Western blotting. The localization of TsCDC37 in adult worms was demonstrated by immunofluorescent technique. Results The reeombinant expression vector was constructed successfully. The reeombinant protein was about M, 52 000, it was then purified and specifically recognized by immunosera of SD rats and sera from patients infected with Taenia solium, Taenia saginata or Taenia asiatica. The immunofluorescence assay revealed that TsCDC37 located at the tegument of T. solium adult and the eggs. Conclusion TsCDC37 gene has been expressed with immunoreactivity. The reeombinant protein is mainly expressed in tegument and egg, and is a common antigen of the three human taenia cestodes.%目的 原核表达猪带绦虫(Taenia solium)细胞分裂周期蛋白37(cell division cycle 37,TsCDC37),并对其进行组织定位和免疫原性研究.方法 通过Blastx分析从猪带绦虫成虫cDNA质粒文库中筛选出 TsCDC37基因,PCR扩增CDC37基因,以pET-28a(+)为载体构建目的基因原核表达体系,在大肠埃希菌(E.coli)BL-21/DE3 中诱导表达,纯化的重组蛋白免疫SD大鼠制备免疫血清,Western blotting分析重组蛋白的免疫原性,免疫组织化学方法观察TsCDC37在

  7. A marca de origem: comparando colleges norte-americanos e faculdades brasileiras The mark of origin: a comparison between colleges in the United States and faculdades in Brazil

    Directory of Open Access Journals (Sweden)

    Arabela Campos Oliven

    2005-05-01

    Full Text Available Colleges nos Estados Unidos e faculdades no Brasil existiam antes da criação de universidades. Instituições radicalmente diferentes, têm marcado profundamente o desenvolvimento da educação superior nos dois países. O college nasceu privado, mais ligado ao seu Board of Trustees (Conselho de Curadores do que à Coroa Inglesa, e com profunda orientação religiosa. As primeiras faculdades no Brasil, criadas por Dom João VI, seguiam o modelo das Grandes Escolas Francesas: eram instituições seculares, de formação de profissionais. Seus professores costumavam ser médicos de renome que se dedicavam tangencialmente às atividades docentes. Nos Estados Unidos, os professores moravam nos colleges com os estudantes e assumiam a responsabilidade na formação do caráter de seus alunos, estando no lugar dos pais (in loco parentis. Este trabalho traça um paralelo entre a educação superior nos Estados Unidos e no Brasil, dando ênfase à influência do modelo de origem no desenvolvimento dos dois sistemas. Procura mostrar como o exemplo das instituições fundantes se constitui em marca indelével, presente nos períodos tanto de continuidade como nos de mudança dos sistemas.Colleges in the United States and faculdades in Brazil were the first higher education institutions in those countries which existed before universities. These two old and traditional institutions, radically different from each other, have marked deeply the development of higher education in both countries. Most colleges were private from the beginning, they used to be more dependent on their Board of Trustees than on the English Crown, having also a profound religious orientation. The first faculdades in Brazil were founded by Dom João VI, Prince Regent, in the beginning of the XIX century, almost two hundred years latter than Harvard. They followed the French Great Schools model: were lay institutions, with a strong professional orientation. Their professors used to

  8. Sequencing Analysis of Mutant Allele $cdc$28-$srm$ of Protein Kinase CDC28 and Molecular Dynamics Study of Glycine-Rich Loop in Wild-Type and Mutant Allele G16S of CDK2 as Model

    CERN Document Server

    Koltovaya, N A; Kholmurodov, Kh T; Kretov, D A

    2005-01-01

    The central role that cyclin-dependent kinases play in the timing of cell division and the high incidence of genetic alteration of CDKs or deregulation of CDK inhibitors in a number of cancers make CDC28 of the yeast \\textit{Saccharomyces cerevisiae }very attractive model for studies of mechanisms of CDK regulation. Earlier it was found that certain gene mutations including \\textit{cdc28-srm} affect cell cycle progression, maintenance of different genetic structures and increase cell sensitivity to ionizing radiation. A~\\textit{cdc28-srm} mutation is not temperature-sensitive mutation and differs from the known \\textit{cdc28-ts }mutations because it has the evident phenotypic manifestations at 30 $^{\\circ}$C. Sequencing analysis of \\textit{cdc28-srm} revealed a single nucleotide substitution G20S. This is a third glycine in a conserved sequence GxGxxG in the G-rich loop positioned opposite the activation T-loop. Despite its demonstrated importance, the role of the G-loop has remained unclear. The crystal stru...

  9. Caos en sistemas clásico-cuánticos

    Science.gov (United States)

    Dirani, L. D.; Núñez, J. A.

    Una de las formas de estudiar la existencia de caos en el comportamiento de un sistema clásico-cuántico, es truncando el espacio de Hilbert del sistema cuántico. El objetivo de este trabajo es analizar como afecta dicho truncamiento en la dinámica del sistema. Para tal fin se propone un Hamiltoniano integrable, cuyos valores de expectación asociados a coordenadas y momentos cuánticos responden, por el teorema de Ehrenfest, a un sistema de dos osciladores clásicos con acoplamiento lineal.

  10. Sistema mundo: la configuración del poder

    OpenAIRE

    Tello, Ángel Pablo

    2006-01-01

    Partiendo de una comparación del sistema internacional de la guerra fría con el modelo actual, se puede remarcar que el sistema mundo actual se caracteriza por la incertidumbre política, la cual se refleja en la incertidumbre estratégica. El actual sistema mundo se puede estudiar tomando en consideración dos cuestiones esenciales: el juego del poder y las fuerzas por un lado, y los escenarios y actores, por otro. La globalización ha hecho que el sistema mundo sea UNO, pero a su vez se descomp...

  11. La formación de un Sistema Solar

    OpenAIRE

    Aparici Benages, Alberto

    2014-01-01

    En este capítulo de Bocados de Ciencia contaremos la historia que lleva desde una nube de gas y polvo hasta una estrella rodeada de un sistema planetario. Hasta hace bien poco todo lo que sabíamos de este proceso lo habíamos de deducir de nuestro propio Sistema Solar, ya que no conocíamos ningún otro sistema en que se hubieran formado planetas. Pero en los últimos veinte años la situación ha cambiado a gran velocidad: hoy descubrimos sistemas planetarios nuevos cada día, y hemos pasado de pen...

  12. Biochemical analysis of the interactions of IQGAP1 C-terminal domain with CDC42

    Institute of Scientific and Technical Information of China (English)

    Sarah; F; Elliott; George; Allen; David; J; Timson

    2012-01-01

    AIM:To understand the interaction of human IQGAP1 and CDC42,especially the effects of phosphorylation and a cancer-associated mutation. METHODS:Recombinant CDC42 and a novel C-termi- nal fragment of IQGAP1 were expressed in,and puri- fied from,Escherichia coli.Site directed mutagenesis was used to create coding sequences for three phos- phomimicking variants(S1441E,S1443D and S1441E/ S1443D)and to recapitulate a cancer-associated mu- tation(M1231I).These variant proteins were also ex- pressed and purified.Protein-protein crosslinking using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide was used to investigate interactions between the C-terminal fragment and CDC42.These interactions were quanti- fied using surface plasmon resonance measurements.Molecular modelling was employed to make predictions about changes to the structure and flexibility of the protein which occur in the cancer-associated variant. RESULTS:The novel,C-terminal region of human IQGAP1 (residues 877-1558)is soluble following expression and purification.It is also capable of binding to CDC42,as judged by crosslinking experiments.Interaction appears to be strongest in the presence of added GTP.The three phosphomimicking mutants had different affini- ties for CDC42.S1441E had an approximately 200-fold reduction in affinity compared to wild type.This was caused largely by a dramatic reduction in the associa- tion rate constant.In contrast,both S1443D and the double variant S1441E/S1443D had similar affinities to the wild type.The cancer-associated variant,M1231I, also had a similar affinity to wild type.However,in the case of this variant,both the association and dis- sociation rate constants were reduced approximately 10-fold.Molecular modelling of the M1231I variant, based on the published crystal structure of part of the C-terminal region,revealed no gross structural changes compared to wild type(root mean square deviation of 0.564over 5556 equivalent atoms).However,pre- dictions of the

  13. Éxito de eclosión del cocodrilo americano (Crocodylus acutus) y la tortuga golfina (Lepidochelys olivacea) en Puerto Vallarta, Jalisco, México. (Hatching success of American crocodile (Crocodylus acutus) and Olive ridley turtle (Lepidochelys olivacea) in Puerto Vallarta, Jalisco, Mexico)

    OpenAIRE

    Cupul-Magaña, Fabio Germán; Aranda-Mena, Oscar Saúl:

    2005-01-01

    Se presentan resultados históricos del éxito de eclosión para la tortuga golfina (2001-2004) y para el cocodrilo americano (2004-2005) en la región costera de Puerto Vallarta, Jalisco, México. Se destaca un aumento en el éxito de eclosión para la tortuga golfina a la largo del período observado, producto posiblemente de las campañas de protección. Por otro lado, el estero “Boca Negra” es considerado como una zona importante para la reproducción del cocodrilo americano.AbstractWe present the h...

  14. Improving effector functions of antibodies for cancer treatment: Enhancing ADCC and CDC

    Directory of Open Access Journals (Sweden)

    Akito Natsume

    2008-12-01

    Full Text Available Akito Natsume, Rinpei Niwa, Mitsuo SatohAntibody Research Laboratories, Research Division, Kyowa Hakko Kirin Co., Ltd.,/Machida-shi, Tokyo, JapanAbstract: As platforms for therapeutic agents, monoclonal antibodies (MAbs have already been approved, and several MAbs have demonstrated clinical effectiveness in a variety of malignancies. However, several issues have also been emerging in antibody therapy, such as high cost and insufficient drug action. Recently, to improve MAb activity in humans, effector functions have been subjects of focus, especially antibody-dependent cell-mediated cytotoxicity (ADCC and complement-dependent cytotoxicity (CDC. Extensive efforts have been made to enhance these effector functions of MAbs, and successful approaches have been reported by us and others, wherein the binding activity of MAbs to FcγRIIIa or C1q is increased by introducing amino acid mutations into heavy chain constant regions or through glyco-modification of Fc-linked oligosaccharides. In addition, one of the next approaches to optimizing therapeutic antibodies would be to combine multiple enhancing modifications into a single antibody platform to overcome the diverse mechanisms of clinical resistance of tumor cells. For this aim, we have recently developed a successful combination composed of ADCC-enhancing modification by the fucose depletion from Fc-linked oligosaccharides and CDC-enhancing modification by IgG1 and IgG3 isotype shuffling in heavy chains, which could be of great value for the development of third-generation antibody therapeutics.Keywords: ADCC, CDC, effector functions, Fc oligosaccharides, IgG isotypes, nonfucosylated IgG

  15. Slit2 inhibits glioma cell invasion in the brain by suppression of Cdc42 activity.

    Science.gov (United States)

    Yiin, Jia-Jean; Hu, Bo; Jarzynka, Michael J; Feng, Haizhong; Liu, Kui-Wei; Wu, Jane Y; Ma, Hsin-I; Cheng, Shi-Yuan

    2009-12-01

    Acquisition of insidious invasiveness by malignant glioma cells involves multiple genetic alterations in signaling pathways. Slit2, a chemorepulsive factor, controls cell migration of neuronal and glial cells during development and inhibits chemotaxic migration of various types of cells in vitro. However, the role of Slit2 in vitro remains controversial, and the biological significance of Slit2 expression in cancer cell invasion in vivo has not yet been determined. In the present study, we characterized the effects of Slit2 expression on the migration and invasion of invasive glioma cells in vitro and in vivo. By reverse transcriptase polymerase chain reaction (PCR) analyses, Slit2 was found to be expressed at lower levels in primary glioma specimens and invasive glioma cells compared with normal human brain cells and astrocytes. Ectopic expression of Slit2 or treatment with recombinant Slit2 on glioma cells attenuates cell migration and invasion through inhibition of Cdc42 activity in vitro. Cellular depletion of Robo1, a cognate receptor for Slit2, prevented Slit2 inhibition of Cdc42 activity and glioma cell migration. In vivo, expression of Slit2 by invasive SNB19 glioma cells markedly inhibited glioma cell infiltration into the brain of mice. Moreover, impediment of glioma cell invasion by Slit2 did not affect the expression of N-cadherin and beta-catenin in glioma cells. These results provide the first evidence demonstrating that Slit2-Robo1 inhibits glioma invasion through attenuating Cdc42 activity in vitro and in the brain. Understanding the mechanisms of Slit2-Robo1 inhibition of glioma cell invasion will foster new treatments for malignant gliomas.

  16. Ten1p promotes the telomeric DNA-binding activity of Cdc13p: implication for its function in telomere length regulation

    Institute of Scientific and Technical Information of China (English)

    Wei Qian; Jianyong Wang; Na-Na Jin; Xiao-Hong Fu; Yi-Chien Lin; Jing-Jer Lin; Jin-Qiu Zhou

    2009-01-01

    In Saccharomyces cerevisiae, the essential gene CDC13 encodes a telomeric single-stranded DNA-binding protein that interacts with Stnlp and Tenlp genetically and physically, and is required for telomere end protection and te-Iomere length control. The molecular mechanism by which Ten1 participates in telomere length regulation and chro-mosome end protection remains elusive. In this work, we observed a weak interaction of Cdc13p and Tenlp in a gel-filtration analysis using purified recombinant Cdc13p and Ten lp. Ten 1p itself exhibits a weak DNA-binding activity, but enhances the telomeric TG1-3 DNA-binding ability of Cdc13p. Cdc13p is co-immunoprecipitated with Ten1p. In the mutant ten1-55 or ten1-66 cells, the impaired interaction between Ten1p and Cdc13p results in much longer telomeres, as well as a decreased association of Cdc13p with telomeric DNA. Consistently, the Ten1-55 and Ten1-66 mutant proteins fail to stimulate the telomeric DNA-binding activity of Cdc13p in vitro. These results suggest that Ten1p enhances the telomeric DNA-binding activity of Cdc13p to negatively regulate telomere length.

  17. Cdc42 and phosphoinositide 3-kinase drive Rac-mediated actin polymerization downstream of c-Met in distinct and common pathways

    DEFF Research Database (Denmark)

    Bosse, Tanja; Ehinger, Julia; Czuchra, Aleksandra;

    2007-01-01

    Activation of c-Met, the hepatocyte growth factor (HGF)/scatter factor receptor induces reorganization of the actin cytoskeleton, which drives epithelial cell scattering and motility and is exploited by pathogenic Listeria monocytogenes to invade nonepithelial cells. However, the precise...... required the simultaneous inactivation of both Cdc42 and PI3-kinase signaling. Moreover, Cdc42 activation was fully independent of PI3-kinase activity, whereas the latter partly depended on Cdc42. Finally, Cdc42 function did not require its interaction with the actin nucleation-promoting factor N...

  18. Cdc14 Early Anaphase Release, FEAR, Is Limited to the Nucleus and Dispensable for Efficient Mitotic Exit.

    Directory of Open Access Journals (Sweden)

    Christopher M Yellman

    Full Text Available Cdc14 phosphatase is a key regulator of exit from mitosis, acting primarily through antagonism of cyclin-dependent kinase, and is also thought to be important for meiosis. Cdc14 is released from its sequestration site in the nucleolus in two stages, first by the non-essential Cdc Fourteen Early Anaphase Release (FEAR pathway and later by the essential Mitotic Exit Network (MEN, which drives efficient export of Cdc14 to the cytoplasm. We find that Cdc14 is confined to the nucleus during early mitotic anaphase release, and during its meiosis I release. Proteins whose degradation is directed by Cdc14 as a requirement for mitotic exit (e.g. the B-type cyclin, Clb2, remain stable during mitotic FEAR, a result consistent with Cdc14 being restricted to the nucleus and not participating directly in mitotic exit. Cdc14 released by the FEAR pathway has been proposed to have a wide variety of activities, all of which are thought to promote passage through anaphase. Proposed functions of FEAR include stabilization of anaphase spindles, resolution of the rDNA to allow its segregation, and priming of the MEN so that mitotic exit can occur promptly and efficiently. We tested the model for FEAR functions using the FEAR-deficient mutation net1-6cdk. Our cytological observations indicate that, contrary to the current model, FEAR is fully dispensable for timely progression through a series of anaphase landmarks and mitotic exit, although it is required for timely rDNA segregation. The net1-6cdk mutation suppresses temperature-sensitive mutations in MEN genes, suggesting that rather than activating mitotic exit, FEAR either inhibits the MEN or has no direct effect upon it. One interpretation of this result is that FEAR delays MEN activation to ensure that rDNA segregation occurs before mitotic exit. Our findings clarify the distinction between FEAR and MEN-dependent Cdc14 activities and will help guide emerging quantitative models of this cell cycle transition.

  19. CDC25B蛋白S149/S321位点突变对小鼠1-细胞期受精卵发育的影响%Mutation of S149 and S321 Sites of CDC25B Protein on Development of 1-Cell Stage of Fertilized Mouse Eggs

    Institute of Scientific and Technical Information of China (English)

    刘超; 肖建英; 孙小涵; 徐晓燕; 于秉治

    2012-01-01

    Objective: To investigate the role of Serl49 of CDC25B in 1-cell stage of fertilized mouse eggs and the relationship between Serl49 and Ser 321 sites. Methods: pBSK-CDC25B-S149A and pBSK-CDC25B-S149A/S321A mutants were constructed and transcribed into mRNAs in vitro. CDC25B-WT-mRNA, CDC25B-S149A-mRNA and CDC25B-S149A/S321A-mRNA were respectively microinjected into the mouse fertilized eggs in Gi phase after superovulation. Furthermore, the cleavage rate, MPF activity and phosphorylation status of CDC2-pTyrl5 in fertilized eggs were observed. Results: Cleavage rate of fertilized eggs in CDC25B-S149A-mRNA injected group was significantly higher than that of CDC25B-WT-mRNA group, and the peak of MPF activity appeared in the CDC25B-S149A-mRNA group, about 1 h early compared with the CDC25B-WT-mRNA group. While the cleavage rate in CDC25B-S149A/S321 A-mRNA group was significantly higher than that in CDC25B-S149A-mRNA group. Conclusion: PKA regulates the early development of mouse embryos by phosphorylation of S149 of CDC25B, and the residue Serl49 of CDC25B cooperates with Ser321, which plays an important role in the regulation of G2/M transition in the mitotic cell cycle of fertilized mouse eggs.%目的:研究小鼠CDC25B蛋白S149位点对小鼠1-细胞期受精卵发育的影响及S149位点与S321位点的关系.方法:构建pBSK-CDC25B-S 149A和pBSK-CDC25B-S149A/S321A突变体,体外转录成mRNA;采用小鼠超排卵技术取G1期受精卵,显微注射CDC25B-WT-mRNA和突变CDC25B-S149A-mRNA、CDC25B-S 149A/S321A-mRNA,观察其对受精卵发育、M期促进因子(MPF)活性及CDC2-pTyr 15磷酸化状态的影响.结果:CDC25B-S149A-mRNA注射组受精卵卵裂率明显高于CDC25B-WT-mRNA注射组,MPF活性提前1h达到高峰;而联合突变体CDC25B-S149A/S321A-mRNA注射组卵裂率又显著高于CDC25B-S149A-mRNA注射组.结论:在小鼠1-细胞期受精卵有丝分裂过程中,蛋白激酶A(PKA)对小鼠CDC25B蛋白S149位点的磷酸化

  20. CDC Vital Signs–Legionnaires’ Disease

    Centers for Disease Control (CDC) Podcasts

    2016-06-07

    This podcast is based on the June 2016 CDC Vital Signs report. People can get Legionnaires’ disease, a serious type of lung infection, from breathing in small water droplets of water contaminated with Legionella germs. Learn what can be done to help prevent Legionnaires’ disease outbreaks and keep people safe.  Created: 6/7/2016 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 6/7/2016.