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Sample records for cclt rabbit experimental

  1. Radiographic assessment of the femorotibial joint of the CCLT rabbit experimental model of osteoarthritis

    International Nuclear Information System (INIS)

    The purposes of the study were to determine the relevance and validity of in vivo non-invasive radiographic assessment of the CCLT (Cranial Cruciate Ligament Transection) rabbit model of osteoarthritis (OA) and to estimate the pertinence, reliability and reproducibility of a radiographic OA (ROA) grading scale and associated radiographic atlas. In vivo non-invasive extended non weight-bearing radiography of the rabbit femorotibial joint was standardized. Two hundred and fifty radiographs from control and CCLT rabbits up to five months after surgery were reviewed by three readers. They subsequently constructed an original semi-quantitative grading scale as well as an illustrative atlas of individual ROA feature for the medial compartment. To measure agreements, five readers independently scored the same radiographic sample using this atlas and three of them performed a second reading. To evaluate the pertinence of the ROA grading scale, ROA results were compared with gross examination in forty operated and ten control rabbits. Radiographic osteophytes of medial femoral condyles and medial tibial condyles were scored on a four point scale and dichotomously for osteophytes of medial fabella. Medial joint space width was scored as normal, reduced or absent. Each ROA features was well correlated with gross examination (p < 0.001). ICCs of each ROA features demonstrated excellent agreement between readers and within reading. Global ROA score gave the highest ICCs value for between (ICC 0.93; CI 0.90-0.96) and within (ICC ranged from 0.94 to 0.96) observer agreements. Among all individual ROA features, medial joint space width scoring gave the highest overall reliability and reproducibility and was correlated with both meniscal and cartilage macroscopic lesions (rs = 0.68 and rs = 0.58, p < 0.001 respectively). Radiographic osteophytes of the medial femoral condyle gave the lowest agreements while being well correlated with the macroscopic osteophytes (rs = 0.64, p < 0

  2. Angiographic technique in rabbits: an experimental study

    International Nuclear Information System (INIS)

    Objective: The rabbit is the most important experimental animal for the basic interventional research, which has displayed the essential value for the interventional experimental diagnosis and therapy in some tumor and vascular disease, while the angiography is the basic imaging examination for the related study. This research attempts to improve and to optimize the angiographic technique for the experimental rabbits, as well as to modify the hemostasis course in order to increase the success rate of angiographic study in rabbits. At the same time. a preliminary study of CTA and DSA in rabbits has also been made in order to get the relative experience. Methods: Fifteen New Zealand white rabbits were anesthetized, which was followed by CTA with the contrast injection through the ear vein catheterization. The diameters of the major blood vessels were measured and their shape and course were observed. An 18 G needle was used to puncture the blood vessels and a 4 F single bend catheter to perform the DSA examination. The groin area was dissected, the femoral artery and femoral vein were separated, and the catheter was inserted with Seldinger technique. For angiography examination, after the femoral artery was successfully punctured, DSA of the aortic arch, dorsal aorta, abdominal aorta, iliac artery, carotid artery, vertebral artery, celiac artery, anterior mesenteric artery and renal artery was carried out in proper sequence and the appearance of pertaining veins were also observed. For venography examination, after the femoral vein was successfully punctured, angiography of the posterior vena cava and pulmonary artery was performed successively. The femoral vein was pressed for a few minutes to stop bleeding, while the femoral artery bleeding was stanched by forceps, local wet compression with thrombin or surgical reconstruction of femoral artery sheath. Results: According to the diameter and shape of the major vessels, the combination of 18 G vascular puncture

  3. Experimental infection of young rabbits with a rabbit enteric coronavirus.

    OpenAIRE

    Descôteaux, J P; Lussier, G.

    1990-01-01

    The clinical signs and lesions caused by the rabbit enteric coronavirus (RECV) were studied in young rabbits orally inoculated with a suspension containing RECV particles. The inoculated animals were observed daily for evidence of diarrhea. Fecal samples and specimens from the small intestine and from the gut associated lymphoid tissue (GALT) were collected from 2 h to 29 days postinoculation (PI) and processed for immune electron microscopy (IEM) and light microscopy. Coronavirus particles w...

  4. Rabbit as an animal model for experimental research

    Directory of Open Access Journals (Sweden)

    Manjeet Mapara

    2012-01-01

    Full Text Available Animal experimentation is carried out in consultation with the veterinary wing but it is essential that be familiar with experimental protocols of animal model to be able to design an approriate study. This is more so in place where the veterinary facilities are not easily available.Span Rabbits are commonly used as subjects for screening implant material. They have gained favour for their numerous advantages even though they should be ideally used prior to testing in a larger animal model. Though experimentation on rabbits seems to be easy there are many pitfalls. Our endeavor in this article is to integrate all the data about maintaining rabbits as a model and to critically analyze it on the basis of our experimentation.

  5. The rabbit as an experimental model in laryngology

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    Carneiro, Christiano de Giacomo

    2009-06-01

    Full Text Available Introduction: In the research in laryngology we normally use animal models. The animal experimentation may also contribute largely for this evolution, mainly for the easy access compared to human larynxes and for they are more easily controlled. Objective: The objective of this work is to analyze the laryngofissure with vocal cords graft as an experimental surgical technique in male adult rabbits. Method: We studied 46 New Zealand albino rabbits submitted to microsurgery in both vocal cords with autologous unilateral or bilateral graft of fat or fascia. Results: There were 4 losses of 3 animals until the first week of the postoperative period and another after 19 days after surgery. In the subsequent animals there were no infection, hematoma or sutures dehiscence. Conclusion: The study enables the conclusion that the experimental laryngofissure in rabbits is a safe method that may be used for laryngological studies.

  6. Effects of Qingkailing on Experimental Allergic Uveitis in Rabbits

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    Experimental allergic uveitis(EAU)in rabbits was induced by singleintraocular injection of bovine serum albumin(BSA).The average of protein con-centration in aqueous humor of untreated group of rabbits was 14.33±1.21mg/mland the count of tritiated thymidine(~3H-TdR)incorporated into lymphocyte T was3,987±1,156cpm/10~6.The specific antibody responses to BSA in the serum andthe aqueous were 0.508±0.034 and 0.369±0.019(OD)respectively.Meanwhile,the effect of Qingkailing on EAU was observed in comparison wi...

  7. Rabbit as an animal model for experimental research

    OpenAIRE

    Manjeet Mapara; Betsy Sara Thomas; Bhat, K M

    2012-01-01

    Animal experimentation is carried out in consultation with the veterinary wing but it is essential that be familiar with experimental protocols of animal model to be able to design an approriate study. This is more so in place where the veterinary facilities are not easily available.Span Rabbits are commonly used as subjects for screening implant material. They have gained favour for their numerous advantages even though they should be ideally used prior to testing in a larger animal model. T...

  8. Apoptotic clearance in rabbits with experimental pulmonary emphysema

    OpenAIRE

    Žunić-Božinovski Snežana; Žunić Svetlana; Mladenović-Đorđević Aleksandra; Ruždijić Sabera; Kanazir Selma

    2011-01-01

    In order to better understand pathogenesis of pulmonary emphysema, the model of experimentally induced pulmonary emphysema in Chinchilla rabbits was used for the estimation of apoptotic clearance of pulmonary tissue. Bronchoalveolar lavage was performed in three groups of animals: experimental group-E on hypercholesterolemic diet (4% edible oil solution of crystalline cholesterol), control group-C1 on standard diet for that animal species and animals on oil...

  9. EXPERIMENTAL MODEL OF ACUTE GLAUCOMA ON RABBIT. HISTOLOGYCAL STUDY

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    Vasile Sirbu

    2007-04-01

    Full Text Available The study was conducted on an experimental model of acute glaucoma on rabbit and investigates the histologycal modifications determined by the sudden increase of the intraocular pressure at the level of the retina.The histologycal analise shows that under conditions of the experiment there are discrete alterations at the level of the retinal layers, without a general modification of the retinal arhitecture.

  10. EXPERIMENTAL MODEL OF ACUTE GLAUCOMA ON RABBIT. HISTOLOGYCAL STUDY

    Directory of Open Access Journals (Sweden)

    Vasile Sarbu

    2007-08-01

    Full Text Available The study was conducted on an experimental model of acute glaucoma on rabbit and investigates the histologycal modifications determined by the sudden increase of the intraocular pressure at the level of the retina.The histologycal analise shows that under conditions of the experiment there are discrete alterations at the level of the retinal layers, without a general modification of the retinal arhitecture

  11. EXPERIMENTAL MODEL OF ACUTE GLAUCOMA ON RABBIT. HISTOLOGYCAL STUDY

    OpenAIRE

    Vasile Sarbu

    2007-01-01

    The study was conducted on an experimental model of acute glaucoma on rabbit and investigates the histologycal modifications determined by the sudden increase of the intraocular pressure at the level of the retina.The histologycal analise shows that under conditions of the experiment there are discrete alterations at the level of the retinal layers, without a general modification of the retinal arhitecture.

  12. Experimental model of cutaneous radiation injury in rabbits

    Energy Technology Data Exchange (ETDEWEB)

    Meirelles, Rafael Panisi de Campos [Universidade Federal de Sao Paulo (EPM/UNIFESP), SP (Brazil). Escola Paulista de Medicina; Hochman, Bernardo [Universidade Federal de Sao Paulo (EPM/UNIFESP), SP (Brazil). Escola Paulista de Medicina. Dept. de Cirurgia; Helene Junior, Americo; Fraga, Murillo Francisco Pires [Faculdade de Ciencias Medicas da Santa Casa de Sao Paulo (FCMSCSP), SP (Brazil). Dept. de Cirurgia. Divisao de Cirurgia Plastica; Lellis, Rute [Faculdade de Ciencias Medicas da Santa Casa de Sao Paulo (FCMSCSP), SP (Brazil). Divisao de Patologia; Ferreira, Lydia Masako, E-mail: rpcmeirelles@yahoo.com.br, E-mail: lydia.dcir@epm.br [Universidade Federal de Sao Paulo (EPM/UNIFESP), SP (Brazil). Escola Paulista de Mediciana. Divisao de Cirugia Plastica

    2013-07-01

    Purpose: to describe an experimental model of cutaneous radiation injury in rabbits. Methods: on this study eight six-month-old New Zealand male rabbits, with an average weight of 2.5kg were used. They were distributed in four groups (n=2 per group). The control group did not receive radiotherapy and the others received one radiotherapy session of 2000, 3000 and 4500 cGy, respectively. Photographic analysis and histopathological evaluation of the irradiated areas were carried out. Results: after 30 days, the animals from the control group had all their hair grown. In spite of that, the animals from group 2000 cGy had a 60-day alopecia and from group 3000 cGy, a 90-day alopecia. After the 30th day, the 3000cGy group demonstrated 90-day cutaneous radiation injuries, graded 3 and 4. One of the animals from group 4500 cGy died on the 7th day with visceral necrosis. The other from the same group had total skin necrosis. A progressive reduction of glands and blood vessels count and an increase on collagen deposition was observed. Conclusion: The proposed experimental model is reproducible. This study suggests that the dosage 4500cGy is excessive and the 3000 cGy is the most effective for this experimental model of cutaneous radiation injury in rabbits. (author)

  13. Experimental model of cutaneous radiation injury in rabbits

    International Nuclear Information System (INIS)

    Purpose: to describe an experimental model of cutaneous radiation injury in rabbits. Methods: on this study eight six-month-old New Zealand male rabbits, with an average weight of 2.5kg were used. They were distributed in four groups (n=2 per group). The control group did not receive radiotherapy and the others received one radiotherapy session of 2000, 3000 and 4500 cGy, respectively. Photographic analysis and histopathological evaluation of the irradiated areas were carried out. Results: after 30 days, the animals from the control group had all their hair grown. In spite of that, the animals from group 2000 cGy had a 60-day alopecia and from group 3000 cGy, a 90-day alopecia. After the 30th day, the 3000cGy group demonstrated 90-day cutaneous radiation injuries, graded 3 and 4. One of the animals from group 4500 cGy died on the 7th day with visceral necrosis. The other from the same group had total skin necrosis. A progressive reduction of glands and blood vessels count and an increase on collagen deposition was observed. Conclusion: The proposed experimental model is reproducible. This study suggests that the dosage 4500cGy is excessive and the 3000 cGy is the most effective for this experimental model of cutaneous radiation injury in rabbits. (author)

  14. Experimental osteoarthritis in rabbits: lesion progression

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    Wilma N.S. Campos

    2013-03-01

    Full Text Available The aim of this study was to evaluate the progression of lesions in different stages of osteoarthritis (OA experimental by radiography (RX, computed tomography (CT, macroscopic and histopathology, linking these different diagnostic methods, helped to provide information that helps the best time for the therapeutic approach. Four experimental periods were delineated at 3, 6, 9 and 12 weeks after induction of OA, known as PI, PII, PIII and PIV, respectively, each with six animals. We evaluated the five compartments of the femorotibial joint: medial femoral condyle (MFC, lateral femoral condyle (LFC, medial tibial plateau (MTP, lateral tibial plateau (LTP and femoral trochlea (FT. Therefore we established an index by compartment (IC and by adding such an index was estimated joint femorotibial (IFT. It was observed that the CFM was the compartment with the highest IC also differed significantly (p0.05 between the PI and PII, however contrary fact occurred between the PII and PIII (p 0.05 between the PI and PII. In the variation of the average interval between periods, there was a higher value between the PIII PIV and for the other intervals of time periods (PI, PII, and PIII-PII. However, these intervals showed no statistically significant difference (p>0.05. Through the RX, CT, macroscopic and histopathological findings, we found similar patterns among individuals within the same period demonstrating a gradual progression of the disease. These results show that between 3 and 6 weeks progression of the lesion is slower and probably also can be reversed in comparison to other ranges where proved further progression between 9 and 12 weeks after induction of trauma OA. These results may provide a better therapeutic approach aimed at reversing the lesions in early stages of OA. We conclude that the interconnection of the four diagnostic methods individually classified into scores, which were unified in both indices in the evaluation by the femorotibial

  15. An Experimental Rabbit Model of Rhegmatogenous Retinal Detachment

    Institute of Scientific and Technical Information of China (English)

    肖青; 曾水清; 黄渝凯; 王静; 李少华; 张缨

    2004-01-01

    An experimental model of rhegmatogenous retinal detachment (RRD) in rabbits was established to simulate the pathophysiologic condition of human RRD. 24 rabbits were randomly divided into 3 groups and underwent vitrectomy with a vitrector and/or retinotomy with a Charles flute needle, with 12 in group Ⅰ (vitrectomy and retinotomy), 7 in group Ⅱ (retinotomy) and 5 in group Ⅲ (vitrectomy). All animals underwent follow up examinations with direct and indirect ophthalmoscopy and fundus photography 12 h and day 1, 3, 5, 7, 10, 14, 21, and 28 after the procedure(s). Retinal changes were recorded. As a result, 10 RRDs were successfully established in group Ⅰ. Direct and indirect ophthalmoscopy and fundus photography demonstrated typical features of RRD. No RRD developed in group Ⅱ and Ⅲ. It was concluded that the experimental rhegmatogenous retinal detachment produced in a rabbit model after vitrectomy with retinotomy in this study was a convenient and reliable one. This RRD model mimicked the typical pathophysiological changes in humans.

  16. Amitriptyline pharmacokinetics in experimental spinal cord injury in the rabbit

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    Reihanikermani H

    2008-01-01

    Full Text Available Previous studies have demonstrated that pharmacokinetic behavior of several drugs such as paracetamol, theophylline, and aminoglycosides are significantly altered in spinal cord injured patients. No pharmacokinetic study of amitriptyline has been performed in patients and experimental models of spinal cord injury. Pharmacokinetic parameters of amitriptyline in orally treated rabbits subjected to laminectomy and spinal cord injury compared with those underwent laminectomy alone. Among twenty four male rabbits were included in this study, nine of them subjected to spinal cord injury at the 8 th thoracic level by knife severance method and six rabbits underwent laminectomy alone (sham group and nine rabbits treated as control. All received a single oral dose of amitriptyline (20 mg/kg 24 h after injury. Blood sampling were done at predetermined times to 36 h after drug administration. Amitriptyline concentration in serum samples was determined by high-performance liquid chromatography. Pharmacokinetic parameters including maximum concentration (C max , time to reach maximum concentration (T max , half life, and the area under the curve to last detectable concentration time point (AUC 0-t were directly determined from the concentration-time curve. Maximum concentration was observed at 6.5 h after administration in sham group with a concentration of 439.6 ng/ml, whereas in SCI group T max was at 2.7 h with a concentration of 2763.9 ng/ml. In control group it was 3.3 h and 396 ng/ml, respectively. In SCI group, AUC was 9465.6 ng.h/ml and half life was 6 h and for control group it was 2817.4 ng.h/ml and 6.4 h, respectively. Statistical analysis of data showed that SCI didn′t induce significant changes in amitriptyline pharmacokinetic parameters.

  17. Experimental bacterial meningitis in rabbit; evaluation with CT and MRI

    International Nuclear Information System (INIS)

    The purpose of this study was to evaluate the usefulness of computed tomography(CT) and magnetic resonance imaging(MRI) in experimental bacterial meningitis. CT and MR images of experimental bacterial meningitis were obtained after inoculation of 1ml suspension of 10-6/ml Staphylococcus aureus directly into the supratentorial arachnoid space of 18 New Zealand white rabbits. Each animal was studied with both pre-enhanced and post-enhanced CT and MRI at 12, 24, 48 hours and 1 week. Cerebrospinal fluid of all of 18 rabbits were sampled and cultured for bacterial growth. All of 18 rabbits had the clinical symptoms such as neck stiffness and anorexia within 24 hours after the inoculation. Cerebrospinal fluid cultures were positive for Staphylococcus aureus growth. Gd-enhanced MRI exhibited diffuse enhancement along the thickened supratentorial meninges earlier than CT. In Gd-enhanced MRI, the mean contrast enhancement along the thickened supratentorial meninges earlier than CT. In Gd-enhanced MRI, the mean contrast enhancement ratio(CER) at supratentorial meninges increased to 1.93 at 12 hours and 2.99 at 24 hours from 1.06 at 0 hour. Histologic evaluation demonstrated inflammatory cell infiltration into the meninges. MRI also identified the complications of meningitis such as ependymitis and hydrocephalus more effectively than CT. These results indicated that Fd-enhanced MRI detectred earlier the abnormal findingfs of bacterial meningitis and evaluated more effectively the complications of meningitis compared with CT. MRI was more useful than CT in evaluation of the bacterial meningitis

  18. Lack of effectiveness of ofloxacin against experimental syphilis in rabbits.

    Science.gov (United States)

    Une, T; Nakajima, R; Otani, T; Katami, K; Osada, Y; Otani, M

    1987-09-01

    Ofloxacin, a new pyridone-carboxylic acid derivative, was evaluated in experimental syphilis in rabbits in comparison with penicillin G. Experimental syphilis was established by intradermal injection of Treponema pallidum subsp. pallidum Nichols. Ten days after infection, the dermal lesions were characterized by syphilitic papula accompanied with central necrosis. These animals were subsequently treated either with ofloxacin twice a day at an oral dose of 10 mg/kg or with penicillin G once a day at an intramuscular dose of 10,000 U/kg for 21 consecutive days. In penicillin G-treated animals, the dermal lesions became smaller as early as day 3 of treatment and almost disappeared during the therapy. In marked contrast to remarkable efficacy of penicillin G was further development of the lesions in ofloxacin-treated animals, showing no difference in pathological manifestations as compared to untreated animals. The results of nontreponemal serologic test correlated well with the response of animals to treatment. PMID:3325066

  19. Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits

    DEFF Research Database (Denmark)

    Mandal, Nakul; Lewis, Geoffrey P; Fisher, Steven K;

    2015-01-01

    vitreous following experimental retinal detachment using a comparative proteomic based approach. Materials and Methods. Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits. Sham surgery was undertaken in five other rabbits that were used as controls. After seven days......, and α-1-antiproteinase F. Conclusions. Proteomic investigation of the rabbit vitreous has identified a set of proteins that help further our understanding of the pathogenesis of rhegmatogenous retinal detachment and its complications....

  20. Magnetic resonance imaging of the experimental atheroma in rabbits

    International Nuclear Information System (INIS)

    The localized potential of the Mn (III) complex of tetrakis (4-sulfonatophenyl) porphyrin (Mn-TPPS4) for a normal aortic wall and experimental atheroma were investigated in twenty-one male New Zealand White rabbits by means of a 2.0 T magnetic resonance imaging system. Forty-eight hours after intravenous administration of 0.25-0.50 mmol/kg of Mn-TPPS4, the selective contrast enhancing effects in a normal aortic wall and the diet-induced atheromas were observed using an ECG-gated T1 weighted sequence. An intense internal surface was observed in the noninvolved lesions and a mild brownish one in the atheromatous lesions. Histological findings revealed a resemblance between these atheromatous lesions and early atheroma. As a result of accumulation of Mn-TPPS4 by the atheroma, identified on macroscopic examination and by an in vitro MR imaging, experimental atheromatous lesions are rendered apparent on in vivo MR imaging. They are significantly enhanced after injection of Mn-TPPS4. These preliminary results indicate that Mn-TPPS4 might be a suitable MRI contrast agent for the localization of the normal aorta and atheroma. However, the 0.25 mmol/kg of Mn-TPPS4 administered to nine animals, caused fatal arthythmia in three of them. (author)

  1. Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits

    OpenAIRE

    Yuichiro Kikkawa; Satoshi Matsuo; Ryota Kurogi; Akira Nakamizo; Masahiro Mizoguchi; Tomio Sasaki

    2014-01-01

    Background. Although relaxin causes vasodilatation in systemic arteries, little is known about its role in cerebral arteries. We investigated the expression and role of relaxin in basilar arteries after subarachnoid hemorrhage (SAH) in rabbits. Methods. Microarray analysis with rabbit basilar artery RNA was performed. Messenger RNA expression of relaxin-1 and relaxin/insulin-like family peptide receptor 1 (RXFP1) was investigated with quantitative RT-PCR. RXFP1 expression in the basilar arter...

  2. Activity of diclazuril against coccidiosis in growing rabbits: experimental and field experiences

    Directory of Open Access Journals (Sweden)

    Monita Vereecken

    2012-12-01

    Full Text Available The efficacy of diclazuril in growing rabbits was investigated under experimental and field conditions. In a first experimental trial, the susceptibility of recent isolated French Eimeria field strains to in-feed use of diclazuril, salinomycin and robenidine was studied in fattening rabbits. Rabbits were challenged at the age of 31 d with a mixed inoculum of Eimeria magna, E. media and E. perforans. Production data and oocyst excretion were compared with an infected-untreated control group and an uninfected-untreated control group. Infection resulted in significantly lower production data and higher oocyst excretion in the infected-untreated control group. Salinomycin and diclazuril treated rabbits were able to control the infection, demonstrated also by comparable weight gain and final weight to those of the uninfected-untreated control rabbits and significantly higher than those of the infected-untreated control rabbits. Based on the production data and oocyst excretion, robenidine was not able to control the infection adequately. Economic performance (weight gain, feed intake, feed conversion and oocyst excretion were significantly worse than in the uninfected-untreated controls. In a second trial, a 1 yr longitudinal study was carried out in Italy to evaluate the excretion of coccidia in growing rabbits from 8 meat farms applying a 2-phase anticoccidial programme (diclazuril and robenidine. Parasitological parameters (oocyst counts and species identification were measured monthly. Seven of the 11 known coccidial rabbit species were identified. Variable levels of oocysts per gram were detected in the farms, but on all farms lower oocyst per gram and a reduced number of Eimeria spp. in rabbit faeces were recorded in the 8-mo treatment period with diclazuril.

  3. Blastema Tissue Formed at Experimentally-Created Rabbit Ear Hole

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    Mohamadreza Baghaban Eslaminejad

    2013-02-01

    Full Text Available   Objective(s: Throughout evolution, mammalians have increasingly lost their ability to regenerate structures however rabbits are exceptional since they develop a blastema in their ear wound for regeneration purposes. Blastema consists of a group of undifferentiated cells capable of dividing and differentiating into the ear tissue. The objective of the present study is to isolate, culture expand, and characterize blastema progenitor cells in terms of their in vitro differentiation capacity.   Materials and Methods: Five New Zealand white male rabbits were used in the present study. Using a punching apparatus, a 4-mm hole was created in the animal ears. Following 4 days, the blastema ring which was created in the periphery of primary hole in the ears was removed and cultivated. The cells migrated from the blastema were expanded through 3 successive subcultures and characterized in terms of their potential differentiation, growth characteristics, and culture requirements. Results: The primary cultures tended to be morphologically heterogeneous having spindly-shaped fibroblast-like cells as well as flattened cells. Fibroblast-like cells survived and dominated the cultures. These cells tended to have the osteogenic, chondrogenic, and adipogenic differentiation potentials. They were highly colonogenic and maximum proliferation was achieved when the cells were plated at density of 100 cells/cm2 in a medium which contained 10% fetal bovine serum (FBS. Conclusion: Taken together, blastema tissue-derived stem cells from rabbit ear are of mesenchymal stem cell-like population. Studies similar to this will assist scientist better understanding the nature of blastema tissue formed at rabbit ear to regenerate the wound.

  4. Mitomycin against posterior capsular opacification: an experimental study in rabbits.

    OpenAIRE

    Haus, C M; Galand, A L

    1996-01-01

    AIMS/BACKGROUND: Posterior capsular opacification is the most common postoperative complication of extracapsular cataract surgery. The purpose of this study was to attempt to inhibit this secondary cataract formation by using mitomycin (an antimitotic drug). METHODS: A solution containing mitomycin was used to perform hydrodissection (with a 5 minute pause) during extracapsular lens extraction in rabbits. This way of administration was chosen to reduce as much as possible drug diffusion into ...

  5. Transcriptome of Treponema pallidum: Gene Expression Profile during Experimental Rabbit Infection†

    OpenAIRE

    Šmajs, David; McKevitt, Matthew; Howell, Jerrilyn K.; Norris, Steven J; Cai, Wei-Wen; Palzkill, Timothy; Weinstock, George M.

    2005-01-01

    RNA transcript levels in the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from experimentally infected rabbits were determined by the use of DNA microarray technology. This characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels for the survival and pathogenesis of this bacterium.

  6. Experimental and finite element analysis of tibial stress fractures using a rabbit model

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    Melanie Franklyn

    2013-01-01

    Full Text Available AIM: To determine if rabbit models can be used to quantify the mechanical behaviour involved in tibial stress fracture (TSF development. METHODS: Fresh rabbit tibiae were loaded under compression using a specifically-designed test apparatus. Weights were incrementally added up to a load of 30 kg and the mechanical behaviour of the tibia was analysed using tests for buckling, bone strain and hysteresis. Structural mechanics equations were subsequently employed to verify that the results were within the range of values predicted by theory. A finite element (FE model was developed using cross-sectional computer tomography (CT images scanned from one of the rabbit bones, and a static load of 6 kg (1.5 times the rabbit's body weight was applied to represent running. The model was validated using the experimental strain gauge data, then geometric and elemental convergence tests were performed in order to find the minimum number of cross-sectional scans and elements respectively required for convergence. The analysis was then performed using both the model and the experimental results to investigate the mechanical behaviour of the rabbit tibia under compressive load and to examine crack initiation. RESULTS: The experimental tests showed that under a compressive load of up to 12 kg, the rabbit tibia demonstrates linear behaviour with little hysteresis. Up to 30 kg, the bone does not fail by elastic buckling; however, there are low levels of tensile stress which predominately occur at and adjacent to the anterior border of the tibial midshaft: this suggests that fatigue failure occurs in these regions, since bone under cyclic loading initially fails in tension. The FE model predictions were consistent with both mechanics theory and the strain gauge results. The model was highly sensitive to small changes in the position of the applied load due to the high slenderness ratio of the rabbit’s tibia. The modelling technique used in the current study could

  7. Experimental model of severe acute pancreatitis in rabbits Modelo experimental de pancreatite aguda grave em coelhos

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    Alberto Goldenberg

    2007-10-01

    Full Text Available PURPOSE: To develop an experimental model of severe acute pancreatitis in rabbits through a pancreatic ductal injection of sodium taurocholate. METHODS: Twenty-four albino rabbits of the New Zealand lineage were distributed into four groups of six animals (A, B, C and S. The rabbits of three experimental groups (A, B and C were submitted to a laparatomy and received a pancreatic ductal injection of 1ml/kg sodium taurocholate 5%. Also, they were submitted to further laparatomies after 4h, 8h and 12h, respectively. The control group (S was subdivided into two groups of three animals: in subgroup S1 only the pancreatic duct catheterization was performed whereas in subgroup S2 the pancreatic duct catheterization as well as an injection of 1ml/kg physiologic solution 0.9% were carried out. After 12 hours, the rabbits were evaluated. In the re-intervention, blood was collected to determine the amylasemia and a pancreatectomy was carried out to investigate interstitial infiltration, steatonecrosis and necrosis of the organ, using an optical microscope. RESULTS: There was an elevation of amylase in all groups thus proving the existence of acute pancreatitis. The size of the interlobular septum increased progressively with a greater variation between group S1 (0.13 and group C (0. 53 (p=0.035. While all the animals in group A exhibited focal cellular necrosis, it was more intense in the rabbits of group B and culminated with a high proportion of severe pancreatic necrosis in group C animals. The difference in the intensity of cellular necrosis showed statistic significance (p=0.001. CONCLUSION: The proposed experimental model demonstrated its reproducibility and effectiveness in producing severe acute pancreatitis in rabbits.OBJETIVO: Desenvolver modelo experimental de pancreatite aguda grave em coelhos por meio da injeção de taurocolato de sódio no ducto pancreático. MÉTODOS: Vinte e quatro coelhos albinos da linhagem Nova Zelândia foram distribu

  8. Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits

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    Yuichiro Kikkawa

    2014-01-01

    Full Text Available Background. Although relaxin causes vasodilatation in systemic arteries, little is known about its role in cerebral arteries. We investigated the expression and role of relaxin in basilar arteries after subarachnoid hemorrhage (SAH in rabbits. Methods. Microarray analysis with rabbit basilar artery RNA was performed. Messenger RNA expression of relaxin-1 and relaxin/insulin-like family peptide receptor 1 (RXFP1 was investigated with quantitative RT-PCR. RXFP1 expression in the basilar artery was investigated with immunohistochemistry. Relaxin concentrations in cerebrospinal fluid (CSF and serum were investigated with an enzyme-linked immunosorbent assay. Using human brain vascular smooth muscle cells (HBVSMC preincubated with relaxin, myosin light chain phosphorylation (MLC was investigated with immunoblotting after endothelin-1 stimulation. Results. After SAH, RXFP1 mRNA and protein were significantly downregulated on day 3, whereas relaxin-1 mRNA was significantly upregulated on day 7. The relaxin concentration in CSF was significantly elevated on days 5 and 7. Pretreatment with relaxin reduced sustained MLC phosphorylation induced by endothelin-1 in HBVSMC. Conclusion. Upregulation of relaxin and downregulation of RXFP1 after SAH may participate in development of cerebral vasospasm. Downregulation of RXFP1 may induce a functional decrease in relaxin activity during vasospasm. Understanding the role of relaxin may provide further insight into the mechanisms of cerebral vasospasm.

  9. The experimental research of isolated limb perfusion on rabbit model

    International Nuclear Information System (INIS)

    Objective: To set up an isolated limb perfusion (LIP) model of rabbit approximately physiological state. Methods: Measuring and calculating the average speed of blood circulation in rabbit's femoral artery with ultrasound, building up an isolated limb of perfusion system with a mini-membrane-oxygenator and a mini pump. Observing the values of blood analysis (pH, PO2 and PCO2) at prior-, mid- and post-operation with differential exchanging speeds of oxygen, and probabilities of circulation failure with differential blood flow speeds, and the glucose change at several points of time and the difference between the groups with and without compensating glucose, and the dimensions of hind leg prior-and post-operation. Results: Results of ultrasound showed that there was no difference in parameter between bilateral femoral arteries giving no effect on the results of differential groups. Total average flow speed was (12.0 +- 1.8) ml/min. Blood analysis; the perfusion without oxygen exchange showed pH was lower than pre-perfusion and physiological low threshold. PO2 was below the normal standard, PCO2 was higher than pre-perfusion and physiological upper threshold. 100 ml/min of oxygen exchange showed pH, PO2 and PCO2 were nearly normal post-perfusion and 200 ml/min group showed pH and PO2 obviously higher than the normal physiological value. The glucose was lower than the normal at 30 min after perfusion, but could be recovered under the compensation of glucose. There was significant difference between the groups processing with and without glucose. There was no circulation failure when the flow speed was in 10 ml ≤ V ≤ 12 ml. No difference of dimension of hind legs appeared prior-and post-procedure. Conclusions: According to the flow speed offered by ultrasound adhered with adjustment during perfusion. The selection of 100 ml/min oxygen-exchange speed and compensation with glucose could set up an ILP model of rabbit, approximately as in the physiological state

  10. Primary and secondary experimental infestation of rabbits (Oryctolagus cuniculus) with Sarcoptes scabiei from a wild rabbit: factors determining resistance to reinfestation.

    Science.gov (United States)

    Casais, Rosa; Dalton, Kevin P; Millán, Javier; Balseiro, Ana; Oleaga, Alvaro; Solano, Paloma; Goyache, Félix; Prieto, José Miguel; Parra, Francisco

    2014-06-16

    Studies of sarcoptic mange and immunity are hampered by lack of mite sources and natural infestation models. We have investigated the clinical and pathological signs, specific IgG response and acquired immunity in naïve New Zealand White rabbits (Oryctolagus cuniculus) experimentally infested with Sarcoptes scabiei originally isolated from a clinically affected free-living European wild rabbit. Twenty rabbits were infested using two methods, direct contact for a 24 h period with a seeder rabbit simulating the natural process of infestation and application of a dressing holding approximately 1800 live mites on each hind limb (foot area) for a 24h period. Eight weeks post infestation, rabbits were treated with ivermectin and infestation cleared. Eight weeks later seventeen previously infested and four uninfested naïve controls were then re-exposed to the same S. scabiei variety using the same methods and followed for another 8 weeks. The progress of the disease was markedly more virulent in the animals infested by contact, indicating that the effective dose of mites managing to thrive and infest each rabbit by this method was higher. Nevertheless, infestation by contact resulted in partial protection to reexposure, rabbits developed high non-protective antibody titres upon reinfestation and presented severe clinical signs. However, rabbits reinfested by dressing developed lower IgG titres, and presented high levels of resistance to reinfestation, which might be due to induction of a strong local cellular response in the inoculation point that killed the mites and resulted in a lower mite effective dose, with subsequent reduced lesion development. Statistical analysis showed that sex, method of infestation and previous exposure are key factors determining the ability of rabbits to develop immunity to this disease. The rabbit-mange model developed will allow the further study of immunity and resistance to this neglected pathogen using a natural host system. PMID

  11. Comparative penetration of azlocillin and mezlocillin into cerebrospinal fluid of normal rabbits and rabbits with experimentally induced Pseudomonas aeruginosa meningitis.

    OpenAIRE

    Hodges, G R; Worley, S E

    1982-01-01

    The impacts of meningeal infection with Pseudomonas aeruginosa and route of drug administration on the penetration of azlocillin and mezlocillin into the cerebrospinal fluid of rabbits were evaluated. The penetration of both agents was increased to a similar degree in rabbits with meningitis compared with normal rabbits. The increase in penetration was greater after intravenous administration than after intramuscular administration.

  12. Experimental coronary sclerosis induced by immobilization of rabbits: A new model of arteriosclerosis

    Science.gov (United States)

    Tyavokin, V. V.; Tjawokin, W. W.

    1980-01-01

    A new method for producing arteriosclerosis with coronary insufficiency in rabbits by means of immobilization is described and discussed. The experimentally induced atherosclerosis develops due to hypodynamics imposed by the reduced muscular activity without overloading with exogenous cholesterol. The atherosclerosis and coronary insufficiency are associated. With variations in the duration and extent of immobilization, coronary insufficiency alone or with atherosclerosis can be produced.

  13. Protein changes in the retina following experimental retinal detachment in rabbits

    DEFF Research Database (Denmark)

    Mandal, Nakul; Lewis, Geoffrey P; Fisher, Steven K; Heegaard, Steffen; Prause, Jan U; la Cour, Morten; Vorum, Henrik; Honoré, Bent

    2011-01-01

    Retinal detachment leads to the widespread cellular remodeling of the retina. The purpose of this study was to identify protein changes that accompany these cellular alterations by comparing the proteomic profiles of sham and experimentally detached rabbit retina. Elucidation of the proteins most...

  14. Quantification of HTLV-I proviral load in experimentally infected rabbits

    Directory of Open Access Journals (Sweden)

    Kindt Thomas J

    2005-05-01

    Full Text Available Abstract Background Levels of proviral load in HTLV-1 infected patients correlate with clinical outcome and are reasonably prognostic. Adaptation of proviral load measurement techniques is examined here for use in an experimental rabbit model of HTLV-1 infection. Initial efforts sought to correlate proviral load with route and dose of inoculation and with clinical outcome in this model. These methods contribute to our continuing goal of using the model to test treatments that alleviate virus infection. Results A real-time PCR assay was used to measure proviral load in blood and tissue samples from a series of rabbits infected using HTLV-1 inocula prepared as either cell-free virus particles, infected cells or blood, or by naked DNA injection. Proviral loads from asymptomatically infected rabbits showed levels corresponding to those reported for human patients with clinically silent HTLV-1 infections. Proviral load was comparably increased in 50% of experimentally infected rabbits that developed either spontaneous benign or malignant tumors while infected. Similarly elevated provirus was found in organs of rabbits with experimentally induced acute leukemia/lymphoma-like disease. Levels of provirus in organs taken at necropsy varied widely suggesting that reservoirs of infections exist in non-lymphoid organs not traditionally thought to be targets for HTLV-1. Conclusion Proviral load measurement is a valuable enhancement to the rabbit model for HTLV-1 infection providing a metric to monitor clinical status of the infected animals as well as a means for the testing of treatment to combat infection. In some cases proviral load in blood did not reflect organ proviral levels, revealing a limitation of this method for monitoring health status of HTLV-1 infected individuals.

  15. Experimental model of a bone gap by radial ostectomy in rabbits

    OpenAIRE

    Antonio Carlos Cunha Lacreta Junior; Everton Regonato; Lucas Bahdour Cossi; Ciro Alexandre Teixeira Cruvinel; Júlio Carlos Canola

    2010-01-01

    A lot of experimental models have been used to study the process of a fracture’s consolidation, but the problem is that due to anatomic, biologic and technical differences, these models do not always have appropriate parameters for the exact species, for which the experiment was done. The rabbit is an experimental model that is widely used in studies involving bone physiopatology in the face of fractures and their different types of treatment, corresponding to approximately 35% of all the mus...

  16. Efficacy of methanolic extract of Terminalia brownii bark and leaves in treating experimentally infected rabbits

    Directory of Open Access Journals (Sweden)

    O. O. Thoria,

    2011-03-01

    Full Text Available The antimicrobial activity of the methanolic extract of Terminalia brownii bark and leaves was tested in rabbits, experimentally infected with Pasteurella multocida strain B2. Two experiments were performed, in one using the bark and in the other using the leaves of the plant. In each experiment 30 rabbits were used and divided into 5 groups 1a, 2a, 3a, 4a, and 5a and 1b, 2b, 3b, 4b and 5b. Each group was injected subcutaneously with 0.2ml of an over night broth culture of Pasturalla Multocida strain B -2 -3 -6 2 (1x10 , 1x10 and 1x10 dilution. Groups 1a and 1b were kept as control. The methanolic extract of Terminalia brownii bark and leaves was prepared as a suspension in normal saline at a concentration of 50mg/ml and given orally by stomach tube at a dose rate of 100 mg/kg body weight to each infected rabbit two days before infection and then every day after infection for 12 days. Blood samples were taken before dosing and then every 3 days after doing for counting leuckocytes and finding the percentages of neutrophils and lymphocytes. The plant extracts were found to be effective when the rabbits were infected with low doses of Pasteurella multocida strain B2. In group 5a and 5b only half of the rabbits (50% died after survival for a number of days, while the other half recovered at the end of therapy. Rabbits of the other infected groups died within 24 hours after infection.

  17. Studies of ventilation and perfusion in rabbits with experimental pulmonary embolism

    International Nuclear Information System (INIS)

    Ventilation and perfusion studies were performed on 38 experimental rabbits with multiple pulmonary emboli. These embolic materials were human thrombin, lipiodol ultrafluide, gelfoam fragments, ivalon fragments, iron powder and VX-2 cancer. Immediately after the embolization, Xe-133 washout study was begun with the intravenous injection of dissolved xenon. Thereafter, perfusion images were obtained using Tc-99m albumin microspheres (7 to 25μm in diameter). When Xe-133 gas was used, the ventilation study consisting of single-breath, equilibrium after 2.5 minutes and sequential washout images, was carried out in the rabbits. Perfusion scintigrams after embolization showed multiple segmental and subsegmental defects of blood flow to the embolic regions within the both lungs. Xe-133 ventilation images showed homogeneous washout. These thromboemboli had almost disappeared perfusion scintigraphically at 24 hours after embolization. However, in a rabbit after embolization with ivalon fragments mixed with iron powders, a lobar defect in the anterior lobe of the left lung persisted perfusion scintigraphically after 24 hours. Xe- 133 washout from the nonperfused area was considerably prolonged at 20 minutes after embolization. In rabbits after diffuse pulmonary oily microembolization, the washout studies after equilibrium breathing of xenon gas showed remarkable delay in clearing the gas from the whole lung. The perfusion images revealed the fissure sign as a linear area of decreased radioactivity which corresponds to the interlobar fissure. (author)

  18. Establishment of a head injury by club model in rabbits and experimental conditions

    International Nuclear Information System (INIS)

    Objective: To establish an animal model to replicate the injury by club in forensic medicine. Methods: Twenty-four New Zealand white rabbits were divided into control group (n=4), minor injury group (n=10), and severe injury group (n=10). Based on the BIM-Ⅱ Horizontal Bio-impact Machine, a self-designed iron bar was used to produce head injury by club. Six hours after injury, all the rabbits were subjected to a CT examination and dissected to observe the injury morphology and undergo routine pathological examination. Four control, six minor and severe rabbits were given moisture content examination. Results: Varying degrees of positive signs of the nervous system were observed in all the injured rabbits within 6 hours. The mortality rate was 1/10 in the minor injury group and 6/10 in the severe injury group. The morphological changes consisted of different levels of scalp hematoma, skull fracture, epidural hematoma, subdural hematoma, subarachnoid hemorrhage and brain injury. The difference in moisture content between the three groups was of statistical significance. Conclusion: Under the rigidly-controlled experimental condition, this animal model produces good reproducibility and stable results. Meanwhile, it can simulate the morphology of injury by club and be used to study the mechanism of injury by club in forensic medicine. (authors)

  19. Effect of the extracts of pumpkin seeds on the urodynamics of rabbits: an experimental study.

    Science.gov (United States)

    Zhang, X; Ouyang, J Z; Zhang, Y S; Tayalla, B; Zhou, X C; Zhou, S W

    1994-01-01

    Pumpkin seeds were prepared into oil n-butyle alcohol and ether extracts. The effects of the three extracts on the urodynamics of rabbits were observed. It was concluded that the oil preparation could remarkably reduce the bladder pressure, increase the bladder compliance, reduce the urethral pressure. Other two kinds of preparations had no effect in this experimental. The mechanisms of the effect of oil preparation on the urodynamics and the prospect of clinical use was discussed. PMID:7760436

  20. Experimental Study on the Preventive Mechanism of Salviae Miltiorrhizae Against Atherosclerosis in Rabbits Models

    Institute of Scientific and Technical Information of China (English)

    李树生; 万磊

    2004-01-01

    Summary: The preventive mechanism of salviae miltiorrhizae (SM) against experimental atherosclerosis (AS) in rabbits models was investigated. The experimental AS rabbit models were reproduced by feeding the high cholesterol diet. The changes of atherosclerotic plaques in normal group, model group and SM treated group were observed. The levels of serum TG, TC, HDL-C and LDL-C were determined. The immunohistochemistry was used to detect the expression of Bcl-2,Bax and IL-6 proteins in atherosclerotic plaques. The results showed that the level of serum TG in SM treated group was significantly lower than in model group (P<0.01). Immunohistochemistry revealed that the expression of Bcl-2, Bax ano IL-6 in model group was significantly higher than in normal group.In the SM group, the expression of Bcl-2 protein was up-regulated and that of Bax was down-regulated. It was suggested that SM could inhibit formation of AS in experimental rabbits. To decrease the expression of Bax and increase the expression of Bcl-2 protein may be one of the mechanisms of SM against atherosclerosis.

  1. An experimentally induced fat embolism in the rabbit lung; high-resolution CT and pathologic findings

    Energy Technology Data Exchange (ETDEWEB)

    Ha, Dong-Ho; Lee, Ki-Nam; Jeong, Jin Sook [Donga Univ. College of Medicine, Pusan (Korea, Republic of)

    2001-02-01

    To assess the high-resolution CT and pathologic findings of fat embolism experimentally induced in rabbit lung. Twelve rabbits were divided into four groups, namely control, 2-hour, 24-hour, and 72-hour, with three rabbits in each, and closed tibiofibular fractures were induced. After the rabbits were sacrificed, high-resolution CT scanning of the artificially inflated lungs was performed, and the CT findings were analyzed by two radiologists. They determined the presence or absence of ground glass opacity or consolidation, the extent of the lesions (using a 10% grading scale), and their distribution, reaching a consensus. The pathologic findings were analyzed using the specimens prepared by H and E and Oil-red O staining. Although the high-resolution CT findings of pulmonary fat embolism were nonspecific, bilateral patchy ground glass opacity (100%), and focal air-space consolidation surrounding the bronchovascular bundle (89%) were most common. In all groups, the occlusion of vessels by fat globules was confirmed by Oil-red O staining. The microscopic findings included focal pulmonary hemorrhage, edema, alveolar collapse, and extensive infiltration of inflammatory cells in the lung parenchyma. The 24-hour group showed more extensive change in high-resolution CT and pathologic findings than did the others. Fat embolism in rabbit lung may occur after closed tibio-fibular fracture. The extent of the lesion revealed by high-resolution CT correlated closely with the pathologic findings. High-resolution CT may thus be helpful for the detection of pulmonary fat embolism and evaluation of its extent.

  2. An experimentally induced fat embolism in the rabbit lung; high-resolution CT and pathologic findings

    International Nuclear Information System (INIS)

    To assess the high-resolution CT and pathologic findings of fat embolism experimentally induced in rabbit lung. Twelve rabbits were divided into four groups, namely control, 2-hour, 24-hour, and 72-hour, with three rabbits in each, and closed tibiofibular fractures were induced. After the rabbits were sacrificed, high-resolution CT scanning of the artificially inflated lungs was performed, and the CT findings were analyzed by two radiologists. They determined the presence or absence of ground glass opacity or consolidation, the extent of the lesions (using a 10% grading scale), and their distribution, reaching a consensus. The pathologic findings were analyzed using the specimens prepared by H and E and Oil-red O staining. Although the high-resolution CT findings of pulmonary fat embolism were nonspecific, bilateral patchy ground glass opacity (100%), and focal air-space consolidation surrounding the bronchovascular bundle (89%) were most common. In all groups, the occlusion of vessels by fat globules was confirmed by Oil-red O staining. The microscopic findings included focal pulmonary hemorrhage, edema, alveolar collapse, and extensive infiltration of inflammatory cells in the lung parenchyma. The 24-hour group showed more extensive change in high-resolution CT and pathologic findings than did the others. Fat embolism in rabbit lung may occur after closed tibio-fibular fracture. The extent of the lesion revealed by high-resolution CT correlated closely with the pathologic findings. High-resolution CT may thus be helpful for the detection of pulmonary fat embolism and evaluation of its extent

  3. Experimental study on the toxicity of lipiodol injected into proper hepatic artery of rabbits

    International Nuclear Information System (INIS)

    Lipiodol is iodized ethyl esters of the fatty acids of poppy-seed oil, and has recently been used as a mediator in combination with anticancer drugs or tagging with radioiodine to treat hepatocellular carcinoma because of higher concentration in tumor tissue compared to normal liver tissue. Author assessed toxic effects and lethal dose of Lipiodol when injected directly into the proper hepatic artery (PHA) of New Zealand white rabbits. 38 rabbits with normal liver function(SGOT < 50 K.U., total bilirubin < 0.1mg/dl) were divided into one control and three experimental groups according to a dosage of Lipiodol into PHA such as A group (10 rabbits) having as control, B group (10 rabbits) having as 0.15 ml/kg of Lipiodol comparable to the average dose to have used as mediator to treat human hepatocellular carcinoma, C group (12 rabbits) having as 1.58 ml/kg of Lipiodol comparable to the average lethal dose of ethiodol to dogs following intravenous injection and D group (6 rabbits) having as lethal dose to be given Lipiodol injection into PHA to death. And A, B, C groups were also subdivided into two groups such as group 1 with no ligation of PHA and group 2 with ligation of PHA after Lipiodol injection respectively. All rabbits were examined the liver function tests, radiographs and CT, and in D group, serial radiographs were taken to evaluate the spreading of Lipiodol. All rabbits except C2 and D groups were sacrificed 6 hours, 24 hours, 5 days, 15 days and 30 days, and then the liver, lungs and kidneys were removed for histologic examination. The results were as follows: 1. The average lethal dose of Lipiodol administered into PHA of white rabbits was 5.44 ml/kg, and the cause of death was pulmonary Lipiodol embolism. 2. The mean survival time of rabbits administered in a dose of 1.58 ml/kg of Lipiodol injection followed by PHA ligation was 25.3 hours, and the cause of death was hepatic failure due to hepatocellular necrosis. 3. On liver function tests, SGOT and

  4. Temporal evolution of mechanical properties of skeletal tissue regeneration in rabbits. An experimental study

    CERN Document Server

    Mokoko, Didier; Chabrand, Patrick

    2007-01-01

    Various mathematical models represent the effects of local mechanical environment on the regulation of skeletal regeneration. Their relevance relies on an accurate description of the evolving mechanical properties of the regenerating tissue. The object of this study was to develop an experimental model which made it possible to characterize the temporal evolution of the structural and mechanical properties during unloaded enchondral osteogenesis in the New Zealand rabbit, a standard animal model for studies of osteogenesis and chondrogenesis. A 25mm segment of tibial diaphysis was removed sub-periosteally from rabbits. The defect was repaired by the preserved periosteum. An external fixator was applied to prevent mechanical loading during osteogenesis. The regenerated skeletal tissues were studied by CT scan, histology and mechanical tests. The traction tests between 7 to 21 days post-surgery were done on formaldehyde-fixated tissue allowing to obtain force/displacement curves. The viscoelastic properties of ...

  5. Experimental infection of eastern cottontail rabbits Sylvilagus floridanus) with infectious bovine rhinotracheitis virus.

    Science.gov (United States)

    Lupton, H W; Reed, D E

    1979-09-01

    Experimental infection of eastern cottontail rabbits (Sylvilagus floridanus) with infectious bovine rhinotracheitis virus caused acute keratoconjunctivitis and a fatal systemic infection. The clinical syndrome was characterized initially by blepharospasm and ocular discharge. The rabbits were markedly depressed on post-exposure day (PED) 5 and were dead or moribund on PED 6. The virus was readily recovered from liver and adrenal gland tissue on PED 6 and from conjunctival swabs on PED 1 to 6. Histopathologic studies revealed a few necrotic foci in the liver and multiple focal to diffuse necrosis of the adrenal glands. Viral isolation and immunofluorescence tests were used to demonstrate a direct association between infectious bovine rhinotracheitis viral antigens and the lesions. PMID:230773

  6. Endovascular treatment of experimental aneurysms in rabbits using Guglielmi detachable coils - a feasibility study

    International Nuclear Information System (INIS)

    The Guglielmi detachable coil (GDC) has been generally accepted for treatment of intracranial aneurysms. Preclinical testing of new coil developments requires animal models of aneurysms which imitate human aneurysms in size, configuration and neck morphology. We assessed in detail the technical requirements and steps for transfemoral treatment of experimentally induced aneurysms at the top of the brachiocephalic trunk (TBC) in rabbits. We created aneurysms in five rabbits by distal ligation and intraluminal incubation of the right common carotid artery with elastase. All animals were treated successfully 2-3 weeks after induction of the aneurysm, with dense packing of the coils. No complications related to the procedures occurred. The study demonstrates that our animal model can be a suitable method for testing the biocompatibility and occlusion rate of new embolic materials. (orig.)

  7. [Effect of trimethylglycine on lipid metabolism in experimental atherosclerosis in rabbits].

    Science.gov (United States)

    Panteleĭmonova, T N; Zapadniuk, V I

    1983-01-01

    It has been shown in adult rabbits aged 8 months with experimental cholesterol atherosclerosis that administration of trimethylglycinee in a dose of 0.5 g/kg reduces the elevated content of total and ester-bound cholesterol, beta-lipoproteins, total lipids in the blood serum and that of total cholesterol and triglycerides in the liver. Little toxicity and high efficacy of trimethylglycin in experimental atherosclerosis make this compound prospective in the light of its use as an antisclerotic agent. PMID:6617841

  8. Interventional fallopian tube recanalization combined with ozone injection in treating chronic obstructive salpingitis: an experimental study in rabbits

    International Nuclear Information System (INIS)

    Objective: To assess the therapeutic value of interventional fallopian tube recanalization (FTR) combined with ozone injection in treating chronic obstructive salpingitis in experimental rabbits in order to provide references for clinical application. Methods: The animal model of chronic obstructive salpingitis was established through interventional uterotubal catheterization in New Zealand white rabbits. A total of 40 rabbit models with salpingitis were randomly and equally divided into four groups: group A (30 μg/ml ozone), group B (40 μg/ml ozone), group C (conventional interventional treatment) and group D (modeling control group). Another ten healthy rabbits were used as a blank control group (group E). After interventional FTR was successfully finished, 10 ml of 30 μg/ml ozone and 40 μg/ml ozone was injected through the catheter into each fallopian tube in the rabbits of group A and group B respectively, while anti-inflammatory drug was given to the rabbits in group C, and 10 ml of saline was injected in the rabbits of both group D and group E. Four weeks after the treatment, the experimental rabbits were sacrificed, and the fallopian tubes were collected and sent for macroscopic and microscopic examination. Results: The recanalization rate of the fallopian tube in group A, B, C and D was 88.9%, 85.0%, 88.9% and 81.3%, respectively. The severity of salpingitis observed in group A, B and C was significantly improved when compared to that observed in group D (P 0.05). Conclusion: Additional use of ozone injection with the concentration of 30 μg/ml or 40 μg/ ml following interventional fallopian tube recanalization management can surely improve the chronic obstructive salpingitis to a certain degree in experimental rabbits. This technique might be advocated in clinical practice. (authors)

  9. Atypical myxomatosis--virus isolation, experimental infection of rabbits and restriction endonuclease analysis of the isolate.

    Science.gov (United States)

    Psikal, I; Smíd, B; Rodák, L; Valícek, L; Bendová, J

    2003-08-01

    Atypical form of myxomatosis, which caused non-lethal and clinically mild disease in domestic rabbits 1 month after immunization with a commercially available vaccine MXT, is described. The isolated myxoma virus designated as Litovel 2 (Li-2) did not induce systemic disease following subcutaneous and intradermal applications in susceptible experimental rabbits but led to the immune response demonstrated by ELISA. No severe disease was induced in those Li-2 inoculated rabbits by challenge with the virulent strains Lausanne (Lu) or Sanar (SA), while the control animals showed nodular form of myxomatosis with lethal course of the illness. Restriction fragment length polymorphism (RFLP) of genomic DNA with KpnI and BamHI endonucleases was used for genetic characterization of the Li-2 isolate, the vaccine strain MXT and both virulent strains Lu and SA, respectively. In general, RFLP analysis has shown to be informative for inferring genetic relatedness between myxoma viruses. Based on restriction endonuclease DNA fragment size distribution, it was evident that the pathogenic strain SA is genetically related to the reference strain Lu and the isolate Li-2 is more related, but not identical, to the vaccination strain MXT. PMID:14628995

  10. Evaluation of arterial impairment after experimental gelatin sponge embolization in a rabbit renal model

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Jung Suk; Lee, Hae Gi; Chun, Ho Jong; Choi, Byung Gil; Choi, Yeong Jin [Seoul St. Mary' s Hospital, College of Medicine, The Catholic University of Korea, Seoul (Korea, Republic of)

    2015-02-15

    Arterial stenosis is a major obstacle for subsequent interventional procedures. We hypothesized that the stenosis is caused by gelatin sponge embolization and performed an experimental study in a rabbit renal model. A total of 24 rabbits were embolized with porcine gelatin sponge particles injected into the renal arteries. Four rabbits were sacrificed on 1 day, 4 days, 1 week, 2 weeks, 3 weeks, and 4 weeks after embolization. Microscopic evaluations were performed on hematoxylin-eosin and smooth muscle actin immunohistochemical stained sections. Gelatin sponge particles were mainly observed in the segmental and interlobar arteries. Transmural inflammation of the embolized arterial wall and mild thickening of the media were observed 1 week after embolization. Resorption of the gelatin sponge and organization of thrombus accompanied by foreign body reactions, were observed from 2 to 4 weeks after embolization. Microscopic images of the 3 weeks group showed vessel lumens filled mostly with organized thrombi, resulting in severe stenosis. Additionally, vessels showed a thickened intima that contained migrating smooth muscle cells and accompanying interruption of the internal elastic lamina. The migrating smooth muscle cells were distributed around the recanalized arterial lumen. Gelatin sponge embolization may induce arterial stenosis by causing organized thrombus and intimal hyperplasia, which consists of migrating smooth muscle cells and intimal collagen deposits.

  11. Evaluation of arterial impairment after experimental gelatin sponge embolization in a rabbit renal model

    International Nuclear Information System (INIS)

    Arterial stenosis is a major obstacle for subsequent interventional procedures. We hypothesized that the stenosis is caused by gelatin sponge embolization and performed an experimental study in a rabbit renal model. A total of 24 rabbits were embolized with porcine gelatin sponge particles injected into the renal arteries. Four rabbits were sacrificed on 1 day, 4 days, 1 week, 2 weeks, 3 weeks, and 4 weeks after embolization. Microscopic evaluations were performed on hematoxylin-eosin and smooth muscle actin immunohistochemical stained sections. Gelatin sponge particles were mainly observed in the segmental and interlobar arteries. Transmural inflammation of the embolized arterial wall and mild thickening of the media were observed 1 week after embolization. Resorption of the gelatin sponge and organization of thrombus accompanied by foreign body reactions, were observed from 2 to 4 weeks after embolization. Microscopic images of the 3 weeks group showed vessel lumens filled mostly with organized thrombi, resulting in severe stenosis. Additionally, vessels showed a thickened intima that contained migrating smooth muscle cells and accompanying interruption of the internal elastic lamina. The migrating smooth muscle cells were distributed around the recanalized arterial lumen. Gelatin sponge embolization may induce arterial stenosis by causing organized thrombus and intimal hyperplasia, which consists of migrating smooth muscle cells and intimal collagen deposits.

  12. Effect of Camel Milk on Oxidative Stresses in Experimentally Induced Diabetic Rabbits

    Directory of Open Access Journals (Sweden)

    Esraa Tantawy

    2010-06-01

    Full Text Available Camel milk has an importance in the treatment of diabetes. It has been shown that the patients who drink camel milk daily, their need to insulin decrease. Therefore, this study aimed to investigate the effect of camel milk in comparison with insulin treatment in experimentally-induced diabetes. This study was carried out on forty male New Zealand rabbits, divided into four groups with ten rabbits in each. The first group G1 was considered as control non-diabetic group and received only normal saline solution. The other animals were injected intravenously with alloxan for induction of diabetes mellitus and then divided into three groups' ten rabbits each as the follows: G2 considered as control diabetic and left untreated, G3 was considered as diabetic and treated with insulin, and G4 was considered as diabetic and received camel milk. At the end of the experiment (4 weeks, blood (whole blood & serum and tissue samples (liver, kidney and pancreas were collected from all the animals for analysis of: enzymatic SOD and catalase, non-enzymatic GSH antioxidant enzyme activities. Serum malondialdeyde, glucose, insulin and lipid profile also were analyzed. The results showed that the camel milk was effective in the treatment of diabetes in comparison to insulin treatment alone. In addition to its hypoglycemic effect, camel milk improved the diabetes-induced oxidative stress. The histopathological evaluations demonstrated that there was a regeneration in β cells and the islets of Langerhans among the pancreatic acini in rabbits receiving camel milk. Our findings suggested that the camel milk administration in case of insulin dependant diabetes mellitus might be recommended as an oral anti-diabetic remedy.

  13. Vascular morphologic and functional effect of endogenous androgens in an experimental atherosclerotic rabbits model

    International Nuclear Information System (INIS)

    Previous clinical and experimental studies suggest that androgens could have adverse, neutral or beneficial effect on atherosclerosis and its clinical manifestations. Methods: an experimental, randomized controlled study in 40 New Zeland white male rabbits was realized. 20 rabbits underwent orchidectomy and 20 were fed with an atherogenic diet for 20 weeks. These were distributed in four groups: 1. non-castrated under normal diet, 2. Castrated under normal diet, 3. non-castrated under atherogenic diet, and 4. Castrated under atherogenic diet. Total cholesterol and free testosterone were measured. After euthanasia, arterial relaxation independent of endothelium was quantified in aorta, as well as the one depending on endothelium, in vitro, and histomorphometric analysis of thoracic aorta were made in order to quantify the atherosclerotic plaque formation. Results: animals that had a normal diet (n=20) had total cholesterol of 51.1 ± 8.5 mg/dl and those with atherogenic diet of 429.2 ± 262.0 mg/dl (p< 0.001). Testosterone levels in the non- castrated group were 2.1 ± 0.3 ng/ml and in the castrated were 0.8 ± 0.4 ng/ml (p= 0.024). In non-castrated rabbits the effect of hypercholesterolemia (366 ± 226.1 mg/dl) inducing atherosclerotic plaque and functional vascular alteration was mild. On the other hand, atherogenic diet in castrated rabbits induced an increment in total cholesterol from 387.6 ± 292.7 mg/dl (p <0.001) and severe morphological changes such as plaque area 2.6 ± 2.3mm (p <0.001), vessel plaque/area 0.25 ± 0.1 (p <0.001) and area index of plaque/area of the media 0.4 ± 0.3 (p <0.001). Endothelium independent relaxation percentage was 85.5 ± 14.3% (p = NS) and endothelium dependent relaxation was 38.5 ± 201% (p = 0.03). Conclusion: This study realized in rabbits demonstrates that endogenous testosterone might have a preventive effect on atherosclerosis and favor endothelium dependent vascular relaxation in the presence of severe

  14. Temocillin efficacy in experimental Klebsiella pneumoniae meningitis after infusion into rabbit plasma to simulate antibiotic concentrations in human serum.

    OpenAIRE

    Woodnutt, G.; Catherall, E J; Kernutt, I; Mizen, L

    1988-01-01

    An infusion system was developed to simulate in the plasma of rabbits the concentrations of temocillin in human serum measured after administration of a 2-g intravenous bolus dose. The efficacy of therapy with this infusion against experimental Klebsiella pneumoniae meningitis was compared with that of a conventional bolus dose to the animals. The marked difference between the elimination half-life (t1/2) of temocillin in rabbit plasma and human serum (0.3 and 5 h, respectively) was reflected...

  15. Surfactant improves lung function and mitigates bacterial growth in immature ventilated rabbits with experimentally induced neonatal group B streptococcal pneumonia

    OpenAIRE

    Herting, E.; Sun, B.; Jarstrand, C; Curstedt, T; Robertson, B.

    1997-01-01

    Aims—To study the influence of surfactant on lung function and bacterial proliferation in immature newborn rabbits with experimental group B streptococcal (GBS) pneumonia.
METHODS—Preterm rabbit fetuses (gest-ational age 28 days) underwent tracheotomy and were mechanically ventilated in a warmed body plethysmograph that permitted measurement of lung-thorax compliance. Fifteen minutes after the onset of ventilation the animals received either GBS or saline intratracheally; at 30 minutes, a bol...

  16. Experimental pleural empyema model in rabbits: Why, how and what are the next steps

    Directory of Open Access Journals (Sweden)

    Cvijanović Vlado

    2014-01-01

    Full Text Available Bacgraund/Aim. The use of new therapeutic methods to prevent development of fibrothorax as the final complication of the human pleural infections requires research with experimental animals. The aim of this study was to standardize the procedures for the establishment of our own experimental model of empyema in rabbits, since it should be able to offer similar conditions found in human pleural infections. Methods. This experiment included 15 chinchilla rabbits, weighing from 2.3 to 2.8 kg. There were 12 rabbits in the experimental group, while 3 rabbits formed the control group. On the first day, we administered 0.4-0.5 mL of turpentine in the right pleural space of the rabbits from the experimental group in order to provoke sterile exudative pleurisy. After 24 h we injected 1 mL of Staphylococcus aureus and 1 mL of Escherichia coli bacteria in the same concentration of 4.5 x 108 bacteria/mL. Thoracocentesis for the pleural fluid analysis was performed 24, 48, 72, and 96 h after bacteria instillation. In these pleural samples we estimated the number of leucocytes and the values of lactate dehydrogenase (LDH, glucose and pH in pleural fluid, as well as the presence of bacteria. We did not protect the animals with antibiotics, and on the day 7 of the experiment they were sacrificed with the lethal dose of barbiturate (iv. The lung from the empyemic side of all experimental animals and the lung of one control animal were histopathologically examined. Results. A total of 4 animals had a small amount of clear pleural fluids or there was no fluid obtained with thoracocentesis 24 and 48 h after the bacteria instillation. after the bacteria instillation. In the remaining 8 rabbits 24 h after bacteria administration the mean values (± SD of the parameters monitored were as follows: Le 34.75 ± 6.13 x 109/L, LDH 17,000 ± 4,69 U/L, glucose 1.23 ± 0.45 mmol/L, and pH 6.975 ± 0.15. The obtained values met the criteria for the evaluation of effusion as

  17. Effect of interventional treatment with p53 on the invasion and metastasis of VX2 liver tumor in experimental rabbits

    International Nuclear Information System (INIS)

    Objective: To investigate the effect of interventional treatment with p53 on the invasion and metastasis of VX2 liver tumor in experimental rabbits. Methods: VX2 carcinoma cells were surgically implanted into the left hepatic lobe in 48 New Zealand white rabbits, and the rabbit hepatic carcinoma models were thus established. The rabbits were randomly divided into 4 groups with 12 rabbits in each group. After hepatic arterial catheterization was completed physiological saline (control group), Lipiodol (Group A), Ad-p53 (Group B) and Lipiodol+Ad-p53 (Group C) were respectively infused into the rabbits of four groups via common hepatic artery. One week after the procedure the rabbits were sacrificed and the livers were removed for the determination of matrix metalloprotein-2 (MMP-2), proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) of the tumor with immunohistochemistry technique. Results: The tumor growth in study groups (group A, B and C) was markedly suppressed, which was significantly different in comparison with that in control group (P 0.05). The positive rates of MMP-2, PCNA and VEGF in group B and C were significantly lower than those in control group (P < 0.05). The positive rates of MMP-2, PCNA and VEGF of the rabbits with metastasis were markedly higher than those without metastasis(P < 0.05). MMP-2 bore a certain relationship with VEGF and PCNA (P < 0.05). Conclusion: The increase of the positive rates of MMP-2, PCNA and VEGF indicates that the tumor possesses higher possibility for developing metastasis, proliferation and vascular formation. The interventional treatment with Adp53 or Lipiodol+Ad-p53 can inhibit the growth, metastasis and vascular formation of VX2 liver tumor in experimental rabbits. (J Intervent Radiol, 2010, 19 : 800-804) (authors)

  18. Endovascular treatment of experimentally induced aneurysms in rabbits using stents: a feasibility study

    International Nuclear Information System (INIS)

    Although Guglielmi detachable coil (GDC) systems have been generally accepted for treatment of intracranial aneurysms, primary stenting of aneurysms using porous stents or implantation of coils after stent placement remains experimental. Testing of these new methods requires an animal model which imitates human aneurysms in size, configuration and neck morphology. We assessed in detail the technical requirements of and steps for transfemoral stent treatment of experimentally induced aneurysms at the top of the brachiocephalic trunk in rabbits. We created aneurysms in ten rabbits by distal ligation and intraluminal digestion of the right common carotid artery with elastase. We treated five animals with porous stents alone, and five with stents plus coiling via the meshes of the stent, which permitted dense packing of coils. No complications related to the procedures occurred. In all animals, even in those treated solely with porous stents, total occlusion of the aneurysm was achieved. Our animal model can be suitable for testing the biocompatibility and occlusion rate of new methods and devices for the treatment of experimental aneurysms. (orig.)

  19. Experimental model of a bone gap by radial ostectomy in rabbits

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Cunha Lacreta Junior

    2010-09-01

    Full Text Available A lot of experimental models have been used to study the process of a fracture’s consolidation, but the problem is that due to anatomic, biologic and technical differences, these models do not always have appropriate parameters for the exact species, for which the experiment was done. The rabbit is an experimental model that is widely used in studies involving bone physiopatology in the face of fractures and their different types of treatment, corresponding to approximately 35% of all the musculoskeletic system’s scientific studies. Several surgical techniques have been used on rabbit’s bone for experimental studies, and the partial ostectomy of the radius bone is one of them. In this study, 14 rabbits (Oryctolagus cuniculus familiaris, males, adults, of white New Zeland breed, neutered, with weight between three and four kilograms, were used. Clinically, the animals did not present any alterations that compromised the study. There were evaluated through radiographic exam on days zero, 30 and 60 after the surgery, visualizing the quality of the gap and the relevant alteration of bone proliferation. The histologic exam elucidated the neoformed bone architecture and its components. The efficacy of the techinique was proved and it could be reproduced for many purposes in orthopedic surgery.

  20. Cholinesterase as inflammatory markers in a experimental infection by Trypanosoma evansi in rabbits

    Directory of Open Access Journals (Sweden)

    Márcio M. Costa

    2012-12-01

    Full Text Available The aim of this study is to evaluate the role of cholinesterases as an inflammatory marker in acute and chronic infection by Trypanosoma evansi in rabbits experimentally infected. Twelve adult female New Zealand rabbits were used and divided into two groups with 6 animals each: control group (rabbits 1-6 and infected group (rabbits 7-12. Infected group received intraperitoneally 0.5 mL of blood from a rat containing 108 parasites per animal. Blood samples used for cholinesterases evaluation were collected on days 0, 2, 7, 12, 27, 42, 57, 87, 102 and 118 days post-inoculation (PI. Increased activity (P0.05 was observed in the encephalic structures. The increased activities of AChE and BChE probably have a pro-inflammatory purpose, attempting to reduce the concentration of acetylcholine, a neurotransmitter which has an anti-inflammatory property. Therefore, cholinesterase may be inflammatory markers in infection with T. evansi in rabbits.O objetivo do presente estudo é avaliar o papel das colinesterases como marcadores inflamatórios nas fases aguda e crônica da infecção por T. evansi em coelhos infectados experimentalmente. Foram utilizados 12 coelhos adultos, fêmeas, da raça Nova Zelândia, divididos em dois grupos: um grupo controle, com seis animais (coelhos 1-6, e um grupo infectado, com seis animais (coelhos 7-12. Os animais pertencentes ao grupo infectados receberam, pela via intraperitoneal, 0,5 mL de sangue de rato contendo 108 tripanossomas por animal. Amostras do sangue utilizado para avaliação das colinesterases foram coletadas nos dias 0, 2, 7, 12, 27, 42, 57, 87, 102 e 118 pós-inoculação (PI. Aumento (P0,05 foi observada nas estruturas encefálicas. O aumento de atividade da AChE e BChE provavelmente tenha finalidade pró-inflamatória, a fim de reduzir as concentrações de acetilcolina, neurotransmissor que apresenta propriedade anti-inflamatória. Portanto, as colinesterases podem ser marcadores inflamatórios na infec

  1. [Use of Plaferon LB for cardiac preconditioning during experimental ischemia/reperfusion injury in rabbits].

    Science.gov (United States)

    Ubilava, T O; Megreladze, I I; Dzhangavadze, M B; Khodeli, N G; Chkhaidze, Z A

    2007-01-01

    The main goal of research was to study potential of Plaferon LB for cardiac preconditioning during experimental ischemia/reperfusion injury in rabbits. 30 rabbits (2.5-3.0 kg) were used in experiment. They were divided in 3 groups and 6 subgroups (n=5). In I group experimental design of m/i was performed by proximal ligation of left coronary artery (LCA) (2-6 hours). In II group on the 2 and 6 hour ligature was removed - reperfusion during 1 hour. In III group before ligation of LCA animals was administered Plaferon LB (0.2 mg/kg). The animals were under electrocardiographic monitoring. Troponin I was measured in blood. In II group after 1 hour of reperfusion Troponin I concentration was higher than in I group after 2 and 6 hours. In II group electrocardiographic data was worsened (rhythm and heart rate). In III group these changes were less marked. Obtained data confirm enhancement of myocardial injury during the reperfusion. Cardiac preconditioning by Plaferon LB significantly decreased pathologic indices. PMID:17921551

  2. Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

    Directory of Open Access Journals (Sweden)

    Inés Martín-Martín

    Full Text Available Sand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop cellular and humoral responses against sand fly saliva. A positive correlation has been observed between the number of bites and antibody levels indicating that anti-saliva antibody response can be used as marker of exposure to sand flies. Little is known about kinetics of antibodies against Phlebotomus perniciosus salivary gland homogenate (SGH or recombinant salivary proteins (rSP. This work focused on the study of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that were experimentally exposed to the bites of uninfected sand flies.Anti-saliva antibodies were evaluated by ELISA and Western blot. In addition, antibody levels against two P. perniciosus rSP, apyrase rSP01B and D7 related protein rSP04 were determined in mice sera. Anti-saliva antibody levels increased along the immunizations and correlated with the number of sand fly bites. Anti-SGH antibody levels were detected in sera of mice five weeks after exposure, and persisted for at least three months. Anti-apyrase rSP01B antibodies followed similar kinetic responses than anti-SGH antibodies while rSP04 showed a delayed response and exhibited a greater variability among sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of exposure and IgG antibodies persisted at high levels, even 7 months post-exposure.Our results contributed to increase the knowledge on the type of immune response P. perniciosus saliva and individual proteins elicited highlighting the use of rSP01B as an epidemiological marker of exposure. Anti-saliva kinetics in sera of experimentally bitten rabbits were studied for the first time. Results with rabbit model provided useful information for a better understanding of the anti-saliva antibody levels found in wild leporids in the human leishmaniasis focus in the Madrid region, Spain.

  3. Experimentally induced pyogenic arthritis of rabbit knees: comparative study of MR imaging and pathology

    International Nuclear Information System (INIS)

    To compare the MR imaging findings of experimentally induced pyogenic arthritis of rabbit knees with the corresponding histopathologic findings. Infection was induced in 20 rabbit knees by direct intra-articular injection of staphylococcus aureus. The animals were divided into four groups of five rabbits each, and spin-echo sagittal T1- and T2-weighted images were obtained 3 days, 1 week, 2 weeks, and 4 weeks, respectively, after staphylococcal inoculation. MR-pathologic correlation was performed, with emphasis on intra-and extra-articular soft tissue lesion characteristics. Soft tissue lesion signal intensity (SI) was classified as low, iso, or high on the basis of that of muscle, and high SI was further subdivided into three categories. At T2-weighted imaging, all soft tissue lesion showed high SI. Pathologic examination revealed the presence of inflammatory cell infiltration (n=2), abscess (n=1), granulation tissue (n=3), fibrosis (n=11), edema (n=4), congestion (n=9), and joint fluid (n=11). Except for the abscess, these lesions were irregular in shape and had variable SI (grade 1-3) and at T2WI could not, therefore, be differentiated. In nine knees, extra-articular soft-tissue lesions were demonstrated at T2WI and correlated with infectious soft tissue lesions such as inflammatory cell infiltration, abscess, granulation tissues and fibrosis; and non-infectious reactive soft tissue changes such as edema and congestion. In pyogenic arthritis, the MR imaging features of soft tissue lesions varied and were nonspecific, depending on the histopathologic abnormalities observed. Our results indicate that in assessing the extent of pyogenic arthritis with MR imaging, caution is required

  4. Experimentally induced pyogenic arthritis of rabbit knees: comparative study of MR imaging and pathology

    International Nuclear Information System (INIS)

    To compare the MR imaging findings of experimentally induced pyogenic arthritis of rabbit knees with the corresponding histopathologic findings. Infection was induced in 20 rabbit knees by direct intra-articular injection of staphylococcus aureus. The animals were divided into four groups of five rabbits each, and spin-echo sagittal T1-and T2-weighted images were obtained 3 days, 1 week, 2 weeks, and 4 weeks, respectively, after staphylococcal inoculation. MR-pathologic correlation was performed, with emphasis on intra-and extra-articular soft tissue lesion characteristics. Soft tissue lesion signal intensity (SI) was classified as low, iso, or high on the basis of that of muscle, and high SI was further subdivided into three categories. At T2-weighted imaging, all soft tissue lesions showed high SI. Pathologic examination revealed the presence of inflammatory cell infiltration (n=2), abscess (n=1), granulation tissue (n=3), fibrosis (n=11), edema (n=4), congestion (n=9), and joint fluid (n=11). Except for the abscess, these lesions were irregular in shape and had variable SI (grade 1-3) and at T2W1 could not, therefore, be differentiated. In nine knees, extra-articular soft-tissue lesions were demonstrated at T2W1 and correlated with infectious soft tissue lesions such as inflammatory cell infiltration, abscess, granulation tissues and fibrosis; and non-infectious reactive soft tissue changes such as edema and congestion. In pyogenic arthritis, the MR imaging features of soft tissue lesions varied and were nonspecine, depending on the histopathologic abnormalities observed. Our results indicate that in assessing the extent of pyogenic arthritis with MR imaging, caution is required

  5. Experimental abscess in the thigh of rabbit : magnetic resonance imaging and pathologic correlation

    International Nuclear Information System (INIS)

    To understand MR imaging characteristics, of abscesses by correlation with pathologic findings, with emphasis on the hypointense rim of the abscess wall on T2-weighted images. We experimentally induced twenty abscesses on both thighs of ten New Zealand white rabbits by innoculation of E. coli. Spin-echo axial T1-weighted images(T1WI), proton density weighted images(PDWI), T2-weighted images(T2WI) and gadolinium enhanced T1WI of two rabbits were each obtained at 1 and 3 days, and at 1, 2 and 4 weeks following innoculation of pathogens. Rabbits were sacrificed after MR imaging, and freezing, sectioning along MR imaging planes and histopathologic examination were subsequently carried out. MR-pathologic correlation was performed, with emphasis on the MR signal characteristics of the abscess wall. In 19 abscesses, necrotic portions except gas showed hypointensity or hyperintensity on T1WI and hyperintensity on T2WI. Peripheral inflammatory reaction showed hypointensity on T1WI, hyperintensity on PDWI and T2WI, and contrast enhancement. Abscess wall showed slightly increased signal intensity on T1WI. A hypointense rim on PDWI and T2WI appeared from 3 days after pathogen innoculation. The rim was thickest at 1 week, and showed multilayers at 2 weeks and double layers at 4 weeks after pathogen innoculation. Cellular and necrotic debris was accumulated at the inner portion of the abscess wall. Inflammatory cells were mainly polymorphonuclear leukocytes and lymphocytes. Macrophages, which appeared at 3 days, suggested active phagocytosis at 1 week after innoculation. Thereafter, inflammatory reactions decreased and fibrosis progressed. The hypointense rim of the abscess wall on T2WI reflects the accumulation of cellular debris, the paramagnetic effect of free radicals due to active phagocytosing macrophages during the abscess forming stage, and fibrosis during the maturation stage

  6. Serial correlation between the ultrasonographic and pathologic findings of intramuscular hemorrhaging in an experimental rabbit

    International Nuclear Information System (INIS)

    To evaluate the serial ultrasonographic findings of experimental intramuscular hemorrhaging to determine if there is a correlation with the pathologic findings. An ultrasonography (US) was performed before and immediately after an intramuscular blood injection in nine rabbits. In addition, follow-up US images were obtained at 1, 4, 7, 10, 14, 21, and 28 days after the intramuscular blood injections in seven of the rabbits. The pathologic specimens, compared to the US findings on each date, and consisted of samples of left thigh muscle. A US, performed immediately after a blood injection, showed two patterns; 6 cases of hyperechoic muscle thickening and 3 cases of hyperechoic muscle thickening with extravasation between the epimysium and muscle bundle or within muscle bundle. A follow-up US showed a marked decrease in intramuscular hemorrhaging and microcalcifications, which appeared on the 4th, 7th and 10th day after the blood injection. The pathologic findings revealed several short echogenic lines in the muscular bundles which were hemosiderin. Moreover, a 28 day follow-up US revealed that the normal findings are correlated with the normal gross pathologic findings. The US findings of the intramuscular hemorrhaging, for each of the follow-up dates, in comparison to the pathologic findings, revealed a high correlation with the pathologic specimens. Consequently, a US transducer with high resolution can be considered as a helpful technique in the diagnosing and evaluating the follow-up treatment of intramuscular hemorrhaging

  7. Induction of corneal collagen cross-linking in experimental corneal alkali burns in rabbits

    Directory of Open Access Journals (Sweden)

    Marcello Colombo-Barboza

    2014-10-01

    Full Text Available Objective: To evaluate the effect of riboflavin-ultraviolet-A-induced cross-linking (CXL following corneal alkali burns in rabbits. Methods: The right corneas and limbi of ten rabbits were burned using a 1N solution of NaOH and the animals were then divided into two groups: a control group submitted to clinical treatment alone and an experimental group that was treated 1 h after injury with CXL, followed by the same clinical treatment as administered to the controls. Clinical parameters were evaluated post-injury at 1, 7, 15, and 30 days by two independent observers. Following this evaluation, the corneas were excised and examined histologically. Results: There were no statistically significant differences in clinical parameters, such as hyperemia, corneal edema, ciliary injection, limbal ischemia, secretion, corneal neovascularization, symblepharon, or blepharospasm, at any of the time-points evaluated. However, the size of the epithelial defect was significantly smaller in the CXL group (p<0.05 (day 15: p=0.008 and day 30: p=0.008 and the extent of the corneal injury (opacity lesion was also smaller (day 30: p=0.021. Histopathology showed the presence of collagen bridges linking the collagen fibers in only the CXL group. Conclusions: These results suggest that the use of CXL may improve the prognosis of acute corneal alkali burns.

  8. Serial correlation between the ultrasonographic and pathologic findings of intramuscular hemorrhaging in an experimental rabbit

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Kyung Ran [National Cancer Center, Seoul (Korea, Republic of); Ryu, Kyung Nam; Park, Ji Seon; Jin, Wook; Sung, Dong Wook; Park, Yong Koo [KyungHee University Medical Center, Seoul (Korea, Republic of)

    2008-05-15

    To evaluate the serial ultrasonographic findings of experimental intramuscular hemorrhaging to determine if there is a correlation with the pathologic findings. An ultrasonography (US) was performed before and immediately after an intramuscular blood injection in nine rabbits. In addition, follow-up US images were obtained at 1, 4, 7, 10, 14, 21, and 28 days after the intramuscular blood injections in seven of the rabbits. The pathologic specimens, compared to the US findings on each date, and consisted of samples of left thigh muscle. A US, performed immediately after a blood injection, showed two patterns; 6 cases of hyperechoic muscle thickening and 3 cases of hyperechoic muscle thickening with extravasation between the epimysium and muscle bundle or within muscle bundle. A follow-up US showed a marked decrease in intramuscular hemorrhaging and microcalcifications, which appeared on the 4th, 7th and 10th day after the blood injection. The pathologic findings revealed several short echogenic lines in the muscular bundles which were hemosiderin. Moreover, a 28 day follow-up US revealed that the normal findings are correlated with the normal gross pathologic findings. The US findings of the intramuscular hemorrhaging, for each of the follow-up dates, in comparison to the pathologic findings, revealed a high correlation with the pathologic specimens. Consequently, a US transducer with high resolution can be considered as a helpful technique in the diagnosing and evaluating the follow-up treatment of intramuscular hemorrhaging.

  9. Gd-DTPA enhanced dynamic renal MR imaging of experimentally induced crescentic glomerulonephritis in rabbits

    International Nuclear Information System (INIS)

    The purpose of this study was to evaluate the utility of Gd-DTPA-enhanced dynamic MRI (DWRI) in early diagnosis and the assessment of disease processing in experimentally-induced crescentic glomerulonephritis (CGN) in rabbits. In six rabbits, CGN was induced by an injection of anti-glomerular basement membrane Ab. A time-signal intensity curve (TSC) was obtained from DMRI on the day before, and at 1, 4, 7, 15, 45 and 113 days after the induction of CGN. Sequential renal biopsies and blood sampling (serum creatinine) were performed on the same days, and the results of DMRI and TSC, were compared. In normal kidneys, sequential DMRI demonstrated the intratubular passage of Gd-DTPA as an inwardly migrating, thin, dark, band pattern. On days 1 and 4, the thin dark band appeared but was poorly defined and TSC revealed a delay in peak time. On days 7 and 15, movement of the band was seen to be weak and slow, and there was no centripetal migration. The maximal signal intensity of TSC was delayed, and the curves declined very slowly. On days 45 and 113, the dark band pattern was very weak and slow, and again there was no contripedal migration. GD-DTPA-enhanced DMRI may be of value in the evaluation of disease processing and the severity of CGN. (author). 26 refs., 4 figs

  10. Experimental study on quantitative analysis of fatty liver by computed tomography in rabbit

    International Nuclear Information System (INIS)

    For the study of correlation of CT number and fatty contact of the liver, total 35 rabbit were used. The rabbits were divided into one control and three experimental groups according to the amount of carbon tetrachloride, administered 1 ml, 2 ml, and 3 ml per kg of body weight respectively. The carbon tetrachloride was administered via intragastric route. CT was performed 72 hours following administration of carbon tetrachloride, and immediately prior to CT scan each rabbit was anesthetized by means of IM injection of ketamine and IV injection of succinylcholine to stop motion and respiration. CT numbers were measured and histological study for the deposition of fat in the liver tissue was done. Also biochemical analysis of lipid in the blood and in the extraction of liver tissue was performed. Comparison of CT number with biochemical and histological data was done. The result was as follows: 1. In each experimental group, the degree of accumulation of fat in the liver was proportional to the amount of carbon tetrachloride. 2. Degree of decreasing CT number was correlated with the increase in the accumulation of fat in the liver. 3. In the control and experimental (1, 2, 3) groups, when the amount of carbon tetrachloride increased, the amount of total lipid, cholesterol and triglyceride on biochemical analysis of the liver increased. Also increase in the amount of plasma lipids on biochemical analysis and great degree of increase in accumulation of fat in the liver on histological examination were seen. But no changes in the amount of phospholipid and protein on biochemical analysis of the liver were noticed. 4. Increase in the amount of total lipid could make a change in the CT number of the liver remarkably (r=-0.63). As the amount of cholesterol and triglyceride increased, the CT number of the liver decreased (r=-0.61 and r=-0.76 respectively). But there was no significant correlation between the amount of protein and phospholipid and the degree of decrease in

  11. Experimental study on the toxicity of povidone-iodine solution in brain tissues of rabbits

    OpenAIRE

    Li, Shu-Hua; Wang, Yu; Gao, Hai-Bin; Zhao, Kun; Hou, Yu-Chen; Sun, Wei

    2015-01-01

    Objective: To determine whether Povidone-iodine was toxic to brain tissues by rinsing the cerebral cortex of New Zealand rabbits with Povidone-iodine Solution of different concentrations. Methods: 12 New Zealand rabbits were randomly divided into 4 groups (Group A, B, C and D, 3 rabbits each group). In each group, the left cerebral cortex of rabbits was rinsed with physiological saline after the craniotomy; in Group A and B, the right cerebral cortex of rabbits was also locally rinsed with Po...

  12. Does longstanding nicotine exposure impair bone healing and osseointegration? An experimental study in rabbits

    DEFF Research Database (Denmark)

    Gotfredsen, Klaus; Lindh, Christian H; Berglundh, Tord

    2009-01-01

    for 32 weeks. The pump delivered 6 microg/kg/min of nicotine for the animals in the test group. Blood samples were collected and plasma cotinine levels were measured monthly. Six months after the commencement of nicotine or saline administration three osteotomy preparations, one in right, femoral...... condyle and two in the right tibia were made. One experimental implant was placed in the femur site and one in the most distal preparation of the tibia. The remaining site in the tibia was left empty. The osteotomy preparation and implant installation procedure was repeated in the left leg of the rabbits...... after 2 weeks. The implants in the tibial site were subjected to removal torque test (RMT) 2 weeks later. Block biopsies of the two other osteotomy sites were prepared for histological analysis. RESULTS: Although no differences in RMT values were found between test and control groups, there was a...

  13. Effect of systemic administration of nicotine on healing in osseous defects. An experimental study in rabbits. Part II

    DEFF Research Database (Denmark)

    Balatsouka, Dimitra; Gotfredsen, Klaus; Sørensen, Lars;

    2006-01-01

    placed mini-osmotic pumps for 8 weeks. The animals underwent three surgical operations during the experimental period, and body weight was registered weekly. Blood samples were collected to determine cotinine and prostaglandin E(2) levels. Bone preparations were made in the right leg of all rabbits after...... 4 weeks and in the left leg after 6 weeks of nicotine/placebo exposure. Thus, 2- and 4-week healing groups were created for the bone defects. After 8 weeks, the animals were killed. Tissue blocks including the bone defects were prepared for histological analysis. RESULTS: The animals in the test......OBJECTIVES: The aim of the present study was to analyze the effect of systemic administration of nicotine on bone healing in osseous defects in the tibia of rabbits. MATERIAL AND METHODS: Sixteen female rabbits received nicotine (n=8; test group) or saline (n=8; control group) via subcutaneously...

  14. Animal model of atherosclerosis using rabbit experimentally induced by combination of X-ray and hypercholesterolemia

    International Nuclear Information System (INIS)

    An attempt was made to prepare an animal model of atherosclerosis similar to human lesions. The experimental animals were male Japanese white rabbits weighting about 2 kg. Hypercholesterolemia was experimentally induced by giving a 1% cholesterol diet. Four weeks later, a single dose of 45 Gy was delivered to the femur to produce vascular changes. Soon after irradiation, immunohistochemical examination revealed the adhesion and invasion of macrophages to endothelial cells, followed by accumulation of foam cells and thickness of the intimal plaques. Three months after irradiation, these thickened plaques became fibrotic, calcified, and necrotic. The tunica media was thinned and the internal elastic lamella was destroyed. Irradiated arteries exhibited not only severe narrowing of the lumen but also aneurysmal dilation and the lesions of the irradiated arteries resembled human atherosclerosis. In conclusion, the atherosclerotic model produced by combining experimental hypercholesterolemia and X-ray irradiaiton may serve as a useful model for studies on atherosclerosis because it can be prepared with no need of complicated or time-consuming procedures. (N.K.)

  15. Experimental West Nile Virus Infection in Rabbits: An Alternative Model for Studying Induction of Disease and Virus Control

    Directory of Open Access Journals (Sweden)

    Willy W. Suen

    2015-07-01

    Full Text Available The economic impact of non-lethal human and equine West Nile virus (WNV disease is substantial, since it is the most common presentation of the infection. Experimental infection with virulent WNV strains in the mouse and hamster models frequently results in severe neural infection and moderate to high mortality, both of which are not representative features of most human and equine infections. We have established a rabbit model for investigating pathogenesis and immune response of non-lethal WNV infection. Two species of rabbits, New Zealand White (Oryctolagus cuniculus and North American cottontail (Sylvilagus sp., were experimentally infected with virulent WNV and Murray Valley encephalitis virus strains. Infected rabbits exhibited a consistently resistant phenotype, with evidence of low viremia, minimal-absent neural infection, mild-moderate neuropathology, and the lack of mortality, even though productive virus replication occurred in the draining lymph node. The kinetics of anti-WNV neutralizing antibody response was comparable to that commonly seen in infected horses and humans. This may be explained by the early IFNα/β and/or γ response evident in the draining popliteal lymph node. Given this similarity to the human and equine disease, immunocompetent rabbits are, therefore, a valuable animal model for investigating various aspects of non-lethal WNV infections.

  16. Preparation of vulnerable plaques in abdominal aorta: an experimental study in rabbits

    International Nuclear Information System (INIS)

    Objective: To establish an animal model of vulnerable plaque in abdominal aorta by pharmacological triggering of atherosclerosis in rabbits. Methods: Thirty New Zealand white rabbits were randomly and equally divided into 3 groups: group A, receiving balloon injury together with high lipid diet;group B, receiving high lipid diet only, and group C, receiving regular diet only. After feeding for 12 weeks, all the rabbits in three groups underwent pharmacological triggering with Chinese Russell's viper venom(CRVV) and histamine to induce the rupture of the plaque and the formation of thrombosis. Results: In 8 survival rabbits of group A, ten lesions with the stenotic degree over 50% were found, which had large lipid core with the thickness of fibrous cap being thinner than 65μm, by which these ten lesions were judged as vulnerable plaques. In 9 rabbits of group B, only 3 vulnerable plaques could be determined,while no single vulnerable plaque could be identified in the rabbits of group C. Conclusion: For establishing an animal model of vulnerable plaque in abdominal aorta in rabbits, balloon injury combined with high lipid diet is a feasible and reliable method. (authors)

  17. Experimental osteoarthritis in rabbits: lesion progression Osteoartrite experimental em coelhos: progressão lesional

    Directory of Open Access Journals (Sweden)

    Wilma N.S. Campos

    2013-03-01

    Full Text Available The aim of this study was to evaluate the progression of lesions in different stages of osteoarthritis (OA experimental by radiography (RX, computed tomography (CT, macroscopic and histopathology, linking these different diagnostic methods, helped to provide information that helps the best time for the therapeutic approach. Four experimental periods were delineated at 3, 6, 9 and 12 weeks after induction of OA, known as PI, PII, PIII and PIV, respectively, each with six animals. We evaluated the five compartments of the femorotibial joint: medial femoral condyle (MFC, lateral femoral condyle (LFC, medial tibial plateau (MTP, lateral tibial plateau (LTP and femoral trochlea (FT. Therefore we established an index by compartment (IC and by adding such an index was estimated joint femorotibial (IFT. It was observed that the CFM was the compartment with the highest IC also differed significantly (p0.05 between the PI and PII, however contrary fact occurred between the PII and PIII (p 0.05 between the PI and PII. In the variation of the average interval between periods, there was a higher value between the PIII PIV and for the other intervals of time periods (PI, PII, and PIII-PII. However, these intervals showed no statistically significant difference (p>0.05. Through the RX, CT, macroscopic and histopathological findings, we found similar patterns among individuals within the same period demonstrating a gradual progression of the disease. These results show that between 3 and 6 weeks progression of the lesion is slower and probably also can be reversed in comparison to other ranges where proved further progression between 9 and 12 weeks after induction of trauma OA. These results may provide a better therapeutic approach aimed at reversing the lesions in early stages of OA. We conclude that the interconnection of the four diagnostic methods individually classified into scores, which were unified in both indices in the evaluation by the femorotibial

  18. Effects of flunixin meglumine on experimental tendon wound healing: A histopathological and mechanical study in rabbits

    Directory of Open Access Journals (Sweden)

    Mehdi Behfar

    2014-12-01

    Full Text Available Tendons are frequently targets of injury in sports and work. Whether nonsteroidal anti-inflammatory drugs (NSAIDs have beneficial effects on tendon healing is still a matter of debate. This study was conducted to evaluate effects of flunixin meglumine (FM on tendon healing after experimentally induced acute trauma. Twenty eight adult male New Zealand White rabbits were subjected to complete transection of deep digital flexor tendons followed by suture placement. Treatment group received intramuscular injection of FM for three days, and controls received placebo. Subsequently, cast immobilization was continued for two weeks. Animals were sacrificed four weeks after surgery and tissue samples were taken. The histological evaluations revealed improved structural characteristics of neotendon formation including fibrillar linearity, fibrillar continuity and neovascularization in treatment group compared to those of controls (p 0.05. Mechanical evaluation revealed significant increase in load-related material properties including ultimate load, yield load, energy absorption and ultimate stress in treatment group compared to those of control group (p 0.05. The present study showed that intramuscular injection of FM resulted in improved structural and mechanical properties of tendon repairs and it could be an effective treatment for acute tendon injuries like severance and laceration.

  19. MR imaging of fracture : MR-pathologic correlation of experimentally produced fractures on rabbit tibias

    International Nuclear Information System (INIS)

    To investigate MR imaging characteristics of the fracture healing process. We performed MR-pathologic correlation of fractures experimentally produced by the three-point beding system in the proximal tibias of 24 New Zealand white rabbits. Axial spin echo T1-weighted image(T1WI), T2-weighted image(T2WI) and T1WI after gadopentetate dimeglumine injection were obtained 1-28 days after fracture MR imaging was followed by freezing, sectioning along the MR imaging planes and histopathologic examinations. Changes in MR signal around the fractures were correlated with histopathologic findings. Hematomas and inflammatory tissue around the fractures, noted one day after fracture, showed slightly increased homogeneous signal intensity on T1WI, heterogineous hyperintensity on T2WI and heterogeneous contrast enhancement. Granulation tissue, cartilage, and immature osteoid, noted 1-4 weeks after fracture, showed iso- or slight hyperintensity on T1WI, hyperintensity on T2WI and contrast enhancement. Hard callus containing ossified osteoid showed hypointensity on both T1WI and T2WI without contrast enhancement. The characteristics of MR signal intensity and contrast enhancing pattern can explain the histopathology of the fracture healing process

  20. Histopathological evaluation of urethroplasty with dorsal buccal mucosa: an experimental study in rabbits

    Directory of Open Access Journals (Sweden)

    Geovanne F. Souza

    2008-06-01

    Full Text Available PURPOSE: Buccal mucosa is a widely accepted tissue for urethroplasty. The exact healing and tissue integration process, mainly the histological characteristics of dorsal buccal mucosa graft urethroplasty when used dorsally to reconstruct the urethral plate has not previously been assessed, and thus we developed an experimental model to address this question. MATERIALS AND METHODS: In 12 New Zealand rabbits (weight 2.5 kg we surgically created a dorsal penile urethral defect. A buccal mucosa graft was sutured to the corpora and tunica albuginea, and the ventral urethra anastomosed to this new urethral plate. The animals were divided in three groups and sacrificed 1, 3 and 6 weeks after surgery (groups 1, 2 and 3. A retrograde urethrogram was obtained at autopsy in the last group and the penis analyzed histologically with hematoxylin-eosin and Masson's staining. RESULTS: The urethrograms showed no evidence of fistula or stricture. In group 1 the histopathological analysis showed submucosal lymph-mononuclear inflammatory edema, numerous eosinophils and squamous epithelium integrated into the adjacent urothelium. In group 2 there was no evidence of an inflammatory response but rather complete subepithelial hyaline healing, which was more marked in group 3. CONCLUSION: Healing of buccal mucosa grafts to reconstruct the urethral plate can be achieved by total integration of the squamous epithelium with the urothelium, maintaining the original histological properties of the graft with no fibrosis or retraction.

  1. Cerebral ischemia in rabbit: a new experimental model with immunohistochemical investigation.

    Science.gov (United States)

    Yamamoto, K; Yoshimine, T; Yanagihara, T

    1985-12-01

    Regional cerebral ischemia was produced in the rabbit by unilateral transorbital occlusion of the middle cerebral artery (procedure I); the middle cerebral and azygos anterior cerebral or anterior communicating artery (procedure II); or the middle cerebral, azygos anterior cerebral or anterior communicating, and internal carotid artery (procedure III). Evolution of ischemic lesions was examined with the immunohistochemical reaction for tubulin. With procedure I, ischemic lesions did not become constantly visible for 6 h in the basal ganglia and for 8 h in the frontoparietal region of the cerebral cortex. With procedure II, it was shortened to 3 h in the basal ganglia and to 6 h in the cerebral cortex. With procedure III, the ischemic lesions were observed in 1 h both in the basal ganglia and in the cerebral cortex as loss of the reaction for tubulin in the neuropil, nerve cell bodies, and dendrites. The evidence of neuronal damage became apparent in the same areas later by staining with hematoxylin-eosin. The experimental model presented here may be suitable for investigation of the mechanism that shifts reversible ischemia to cerebral infarction and for evaluation of the effectiveness of pharmacological intervention. PMID:3932374

  2. Measuring of the airway dimensions with spiral CT images: an experimental study in Japanese white big-ear rabbits

    International Nuclear Information System (INIS)

    Objective: To measure the length, angle and their correlation of the main anatomical dimensions of the trachea and bronchus in experimental Japanese white big-ear rabbits with the help of spiral CT 3D images, in order to lay the foundation of treating the airway disorders with stenting in animal experiment. Methods: Multi-slice CT scanning of cervico-thoracic region was performed in 30 healthy adult Japanese white big-ear rabbits, the longitudinal, transversal dimensions of the trachea, the glottis-carina length, the inner diameter and length of bronchi, and the angle formed by bronchial long axis and sagittal section were measured. Results: No significant difference was found in the inner diameters of various parts of the trachea and upper apical bronchi. The angle formed by bronchial long axis and sagittal section were smaller than that of the left ones. And the inner diameters of the right main bronchus was bigger than the left ones. Conclusion: The complex branching structure of the rabbit airway tree can be well displayed on spiral CT 3D images. Through measuring and statistical analysis of the results the authors have got a regressive equation for estimating the value of the inner diameter, length, angle, etc. concerning the airway tree, which is very helpful for providing the useful anatomical parameters in rabbit experiment. (authors)

  3. Experimental Evaluation Of A Herbal Cream (SK-AC On Acetylated Lanolin Alcohol-Induced ACNE In Rabbit

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    Mitra S K

    1998-01-01

    Full Text Available Experimental studies were conducted in four groups of rabbits using external ear canal for inducing comedogenesis and its correction by a herbal formulation, viz., SK-AC cream. The result brought out that the SK-AC cream restricted the comedogenesis induced by acetylated lanolin alcohol when compared with other defined groups. Microcomedo being the basic pathology of acne, the outcome of this experiment pointed out the usefulness of SK-AC cream for the treatment of acne.

  4. Experimental Evaluation Of A Herbal Cream (SK-AC) On Acetylated Lanolin Alcohol-Induced ACNE In Rabbit

    OpenAIRE

    Mitra S.K; Seshadri J S; Uaupa Venkatesh U; Venkataranganna M V; Gopumadhavan S; Sundaram R

    1998-01-01

    Experimental studies were conducted in four groups of rabbits using external ear canal for inducing comedogenesis and its correction by a herbal formulation, viz., SK-AC cream. The result brought out that the SK-AC cream restricted the comedogenesis induced by acetylated lanolin alcohol when compared with other defined groups. Microcomedo being the basic pathology of acne, the outcome of this experiment pointed out the usefulness of SK-AC cream for the treatment of acne.

  5. Differential effect of Pistacia vera extracts on experimental atherosclerosis in the rabbit animal model: an experimental study

    OpenAIRE

    Halabalaki Maria; Magiatis Prokopios; Chatziioannou Achilles; Papalois Apostolos; Iliopoulos Dimitrios; Karatzas Theodore; Agrogiannis George; Georgopoulou Katerina; Marinou Katerina A; Tsantila Nektaria; Skaltsounis Leandros A; Patsouris Efstratios; Dontas Ismene A

    2010-01-01

    Abstract Background Lipid-enriched diets and oxidative stress are risk factors for the development of atherosclerosis. The effects of the methanolic (ME) and cyclohexane (CHE) extracts of the Pistacia vera nut, often included in the Mediterranean diet, were studied in the rabbit model of atherosclerosis. Methods and results Twenty-four New Zealand White rabbits received atherogenic diet (Control Group), supplemented with ME (Group ME) or CHE (Group CHE) for 3 months. Previously, a GC-MS and a...

  6. Studies on experimental infection of rabbits with irradiated metacercariae of Fasciola giganticas Cobbold, 1885

    International Nuclear Information System (INIS)

    Worm burden, gross pathology and serological response of rabbits infected with gamma irradiated metacercariae of Fasciola gigantica has been studied with a view to prepare a vaccine against the pathogen. Infection with metacercariae irradiated at 2 or 3 kr caused reduced worm burden and gross pathology and produced antibody titres comparable to the titres in rabbits infected with normal cysts. Infection with metacercariae irradiated at 4 kr resulted in total absence of worm burden and caused no rise of antibody titre in the sera of rabbits. In every case after infection, worm burden was progressively eliminated over long duration. The pathogenicity was comparatively severe in rabbits infected with normal cysts. (M.G.B.)

  7. Injectable hyaluronic-acid-doxycycline hydrogel therapy in experimental rabbit osteoarthritis

    OpenAIRE

    Lu, Hsien-Tsung; Sheu, Ming-Thau; Lin, Yung-Feng; Lan, Jai; Chin, Yi-Ping; Hsieh, Ming-Shium; Cheng, Chao-Wen; Chen, Chien-Ho

    2013-01-01

    Background Osteoarthritis (OA) is a common joint disease that causes disabilities in elderly adults. However, few long-lasting pharmacotherapeutic agents with low side effects have been developed to treat OA. We evaluated the therapeutic effects of intra-articular injections of hydrogels containing hyaluronic acid (HA) and doxycycline (DOX) in a rabbit OA model. Results Thirteen week old New Zealand White rabbits undergone a partial meniscectomy and unilateral fibular ligament transection wer...

  8. Preparation of bifocal hepatic VX2 tumor model in experimental rabbits by using CT-guided trochar implantation technique

    International Nuclear Information System (INIS)

    Objective: To discuss the feasibility of CT-guided tumor cell implantation through trochar to establish bifocal hepatic VX2 tumor model in experimental rabbits. Methods: Thirty health New Zealand rabbits were randomly divided into two groups: percutaneous puncture group and laparotomy group. CT-guided tumor cell implantation through trochar was carried out for the rabbits in percutaneous puncture group, while conventional tumor cell implantation was performed for the rabbits in laparotomy group. Tumor cells were implanted into both right and left hepatic lobes to create bifocal hepatic VX2 tumor model in experimental rabbits. MRI was performed two weeks after the procedure. The unifocal and bifocal tumor formation rate, tumor satisfaction rate, tumor volume, operating time, death rate and abdominal implantation metastasis rate were observed, the results were compared between two groups. Results: The unifocal tumor formation rate for percutaneous puncture group and laparotomy group was 80.0% and 83.3% respectively (P>0.05). The bifocal tumor formation rate for percutaneous puncture group and laparotomy group was 73.3% and 75% respectively (P>0.05). The tumor satisfaction rate for percutaneous puncture group and laparotomy group was 80.0% and 33.3% respectively (P>0.05). The operation time was shorter in percutaneous puncture group than in laparotomy group [(14.6±7.3) min vs. (37.3±21.7) min, P3 and (1790±1080) mm3 respectively (P>0.05). No significant difference in death rate existed between two groups (0% vs. 20%, P>0.05). The abdominal implantation metastasis rate for percutaneous puncture group and laparotomy group was 20% and 0% respectively (P>0.05). Conclusion: In preparing bifocal hepatic VX2 tumor model in experimental rabbits, CT-guided tumor cell implantation through trochar can provide higher tumor formation rate as well as tumor satisfaction rate. This technique is time-saving and easily-manipulated. It can be used as an alternative for conventional

  9. A comparative study of MRI findings of experimentally induced rapid progressive glomerulonephritis (RPGN) and acute tubular necrosis (ATN) in rabbits

    International Nuclear Information System (INIS)

    To evaluate the usefulness of MR imaging in the differential diagnosis of the underlying causes of early-stage acute renal failure (ARF) by comparing the MRI findings of experimentally-induced crescentic glomerulonephritis (CGN) and acute tubular necrosis (ATN) in rabbits. Experimental CGN was induced by injecting anti-glomerular basement membrane antibody into six rabbits, and ATN by injecting glycerol solution into six rabbits. A normal control group of three rabbits was also used. Renal MR imaging (T1-and T2-weighted coronal images and dynamic MRI : DMRI) was performed the day before, and one,four, and seven days after the induction of CGN; and immediately before, and four, and eight hours, and one, four, and seven days after the induction of ATN. Sequential renal gunbiopsies and blood sampling (serum creatinine, sCr) were performed. Renal area, corticomedullary differentiation (CMD), and the passage of Gd-DTPA (patter of dark band), as seen on MRI, were analyzed and correlated with serial change of sCr. In normal kidneys, CMD was clearly apparent on both T1-and T2-weighted images. DMRI demonstrated a progressively inwardly migrating dark band in the kidneys. CMD was relatively clearly demonstrated in the ATN group but less clearly identified in the CGN group. Renal size (area) and sCr gradually increased in both the CGN and ATN groups, and dark bands were moderately to poorly defined in both. We conclude that DMRI could be used to differentiate and evaluate disease processing and compromised renal function in cases of CGN and ATN. On T1-and T2-weighted images, CMD was relatively well preserved in the ATN group, but was less clear in the CGN group. These MRI findings may be helpful for differentiation of the underlying causes of early-stage ARF, particularly between CGN and ATN

  10. EFFECT OF FLUORIDE ON KIDNEY OF ALBINO RABBIT-AN EXPERIMENTAL STUDY

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    Santosh K.

    2014-08-01

    Full Text Available BACKGROUND: Fluoride is present in environment in various forms and ingested by man from solid foods, drinks, drinking water and inhaled from the air. Out of these, fluoride is present in large quantities in dissolved state in many sources of drinking water producing toxicity in man. Fluoride, being excreted mainly through the kidneys, seems to damage it causing renal dysfunction. Kidneys are among the most sensitive body organs in their histopathological and functional responses to excessive amounts of fluoride. Trace elements are essential and beneficial to human being in minute concentrations. However, intake in large quantities produces adverse and toxic effects on our body. OBJECTIVE: The present study was designed to investigate the toxic effects (evaluated as histopathological changes of sodium fluoride on the kidney in albino rabbit. MATERIALS AND METHODS: Total 40 albino rabbits were used for this study, among them 8 rabbits were taken in the control group (Group A and16 rabbits each were taken in both group B and group C who were administered low and high dose of fluoride respectively. After 2 weeks interval, up to 16 weeks the histology of the kidney of each group of the rabbit was studied for histological analysis. RESULT: Histological changes in the kidneys of both Group B and Group C rabbits, following continuous daily exposure to sodium fluoride solutions in two different doses (0.5% solution for Group B and 3% solution for Group C for different durations of time were studied in detail and compared with those of the controls (Group A. CONCLUSION: It is concluded that Sodium Fluoride solution in high doses for prolonged period has a definite adverse effect on the renal parenchyma.

  11. Comparative Efficacies of Terbinafine and Fluconazole in Treatment of Experimental Coccidioidal Meningitis in a Rabbit Model

    OpenAIRE

    Sorensen, Kevin N.; Sobel, Raymond A.; Clemons, Karl V.; Calderon, Leilani; Howell, Kimberley J.; Irani, Plomarz R.; Pappagianis, Demosthenes; Williams, Paul L.; Stevens, David A.

    2000-01-01

    A rabbit model of coccidioidal meningitis was used to compare the therapeutic efficacies of terbinafine (TBF) and fluconazole (FCZ). Hydrocortisone acetate-treated New Zealand White male rabbits were infected intracisternally with either 2.2 × 104 or 6.4 × 104 Coccidioides immitis arthroconidia. Oral treatment with polyethylene glycol 200 (PEG) twice daily (n = 8), TBF twice daily (n = 9; 200 mg/kg of body weight/day), or FCZ once daily (n = 8; 80 mg/kg/day) began on day 5 and continued for 2...

  12. Virological and clinico-pathological features of orf virus infection in experimentally infected rabbits and mice.

    Science.gov (United States)

    Cargnelutti, J F; Masuda, E K; Martins, M; Diel, D G; Rock, D L; Weiblen, R; Flores, E F

    2011-01-01

    Many aspects of the biology of orf virus (ORFV) infection remain poorly understood and attempts to establish animal models have yielded conflicting and non-reproducible results. We herein describe the characterization of ORFV infection and disease in rabbits and mice. A protocol of intradermal inoculation was employed to inoculate 10(8.5)TCID₅₀/mL of ORFV strain IA-82 in the skin of ears, of the back and labial commissures. All inoculated rabbits presented a clinical course characterized by erythema, macules, papules/vesicles or pustules that eventually dried originating scabs. Local signs started around days 3 and 4 post-inoculation (pi) and lasted 3-10 days. Virus was recovered from lesions between days 2 and 14pi. Histological examination of lesions revealed focal proliferative dermatitis with ballooning degeneration and eosinophilic intracytoplasmic inclusion bodies in keratinocytes, histological hallmarks of contagious ecthyma in sheep. A similar, albeit milder clinical course occurred in 5/10 inoculated mice; virus was recovered from lesions from three animals. Inoculated lambs - used as controls - developed severe lesions of contagious ecthyma. VN tests performed at day 28pi failed to detect neutralizing antibodies in all inoculated animals. In contrast, convalescent rabbit sera were positive by ELISA at dilutions from 100 to 400. These results show that rabbits are susceptible to ORFV infection and thus may be used to study selected aspects of ORFV biology. PMID:20833245

  13. Experimental Study of Percutaneous Ethanol Injection in the Rabbit Liver : Correlations Between Sonography and Pathology

    International Nuclear Information System (INIS)

    To investigate the sonographic change and histopathologic correlation after percutaneous ethanol injection(PEI) of normal rabbit liver. Seven rabbits with normal livers were injected ethanol percutaneously under the guidance of ultrasound. Ethanol was injected to five rabbit livers at two different sites with one week interval in two week time period. The echogenecity of the lesion was compared with that of the adjacent normal liver parenchyma and classified into hypo-, iso-, and hyper echoic pattern. The resected specimens were analyzed according to the presence of coagulation necrosis, liquefactive necrosis, and granulation tissue. Sonography after PEI showed hyper echoic focus in all initial injection site which became isoechoic to the surrounding liver parenchyma with time, except for one case. However, one of the lesions became hypoechoic in a week. The isoechoic lesions on sonography were coagulation necrosis and granulation tissue on pathologic correlation. There was also more severe, periportal fibrosis within parenchyma adjacent isoechoic lesion, which became abundant with respect to time. The hypoechoic lesion was correlated with liquefactive necrosis. Histopathologic findings of normal rabbit livers after PEI are coagulation necrosis and granulation tissue. Although the histopathologic changes become progressive with time, the sonographic findings remain isoechoic to the surrounding liver parenchyma. Hypoechogenecity due to liquefactive necrosis may be helpful to differentiate the lesion from normal hepatic parenchyma

  14. A new liquid embolic agent (embol) for transcatheter renal artery embolization: an experimental study in rabbit

    International Nuclear Information System (INIS)

    To evaluate the effectiveness and safety of a new liquid embolic agent in renal arterial embolization in the rabbit, and its clinical applicability. A new embolic agent, Embol, was obtained by partial hydrolysis of polyvinyl acetate and dissolved in a mixture of 45% ethanol and 55% non-ionic contrast medium. Its radio opacity was therefore good. An average of 0.8 cc(0.5-0.9cc) of Embol was used to embolize the renal artery of one kidney in 15 rabbits. The immediate effect of this was examined angiographically 5 minutes after the procedure. To permit histologic examination, five rabbits in each group were sacrificed 3 days (I), 2 weeks (II), and 4 weeks (III) after embolization: prior to embolization and prior to sacrifice, one rabbit in each group underwent renal scanning, and prior to sacrifice all underwent follow-up angiography. In three rabbits, blood urea nitrogen (BUN), creatinine, sodium (Na), and potassium (K) levels were measured before and 1,3,5,7 and 14 days after embolization. Embol was easy to use and its radiopacity was good. Five minutes after embolization, angiography showed that total occlusion of the main renal or interlobar artery had been achieved in all rabbits. Serum BUN, creatinine, Na and K levels were within normal limits. Follow-up angiogram obtained in each group showed persistent occlusion of the renal artery in all but one rabbit in group I and one in group III. Renal scans revealed no evidence of radionuclide uptake in embolized kidneys, which were slightly enlarged in group I but became gradually smaller in groups II and III. In all animals, histologic examination showed diffuse coagulation necrosis of the embolized kidneys and in group III the cortex of these was extensively calcified. In group I the renal artery showed an apparently fresh occluding thrombosis, and in groups II and III a completely organized thrombosis was present. In group III this was calcified. Because of its good radio opacity, Embol is easy to contrast, and is

  15. Efficacy of pamiray as a nonionic intravenous contrast material; experimental study suing normal rabbits

    International Nuclear Information System (INIS)

    To evaluate the efficacy of Pamiray as an intravenous nonionic contrast material by comparing it with an established nonionic contrast material (Iopamiro). Forty adult New Zealand white rabbits weighing about 3.0 kg were used in this study. Aortography and urography (scout, 5, 15 and 30 minutes delay after injection) were performed in ten rabbits with Pamiray (370 mgI/mL) and in other ten rabbits with Iopamiro (370 mgI/mL). All twenty rabbits underwent abdominal triple-phase spiral CT before and 5 and 30 seconds after injection of the contrast material. The degree of contrast enhancement seen on aortograms and intravenous urograms was determined using a three-point scale by two radiologists, who reached a consensus. CT attenuation expressed in Hounsfield units (HU) was measured using the regions of interest (ROIs) facility in the liver, bilateral kidneys, aorta, portal vein, and inferior vena cave. The Mann-Whitney U test was used in image evaluation for intergroup comparisons. There was no significant difference between the two groups in the degree of contrast enhancement seen on aortograms. In urography, however, Iopamiro was superior to Pamiray in demonstrating calyces of the right kidney on 15-minute delay images and Pamiray was superior to Iopamiro in demonstrating the right ureter and the renal pelvis on five minute-delay images and the left ureter on 30 minutes delay (p less than 0.05). Peak enhancement of the aorta during the first phase of abdominal CT was higher in the group in which Pamiray was used. When normal rabbits were used for aortography, abdominal CT, and urography, Pamiray provided more effective contrast enhancement than a previously used nonionic contrast material, namely Iopamiro. There were, however, slight differences in the enhancement features of renal calyces and ureters seen on 5- and 15-minute urograms. (author)

  16. Low-Level Laser Therapy: An Experimental Design for Wound Management: A Case-Controlled Study in Rabbit Model

    OpenAIRE

    Hossein Hodjati; Siamak Rakei; Hamed Ghoddusi Johari; Bita Geramizedeh; Babak Sabet; Sam Zeraatian

    2014-01-01

    Background: There is a wide array of articles in medical literature for and against the laser effect on wound healing but without discrete effect determination or conclusion. This experimental study aims to evaluate the efficacy of low-level laser therapy on wound healing. Materials and Methods: Thirty-four rabbits were randomly enrolled in two groups after creating a full thickness of 3 × 3 cm wound. The intervention group received low density laser exposure (4 J/cm 2 ) on days 0, 3 and 6 wi...

  17. Hepatic artery embolization using thermosensitive and slow-release drug as embolic agents: an experimental study in rabbits

    International Nuclear Information System (INIS)

    Objective: To explore the feasibility and effect of hepatic artery embolization by using thermosensitive and slow-release drug as embolic agents in experimental rabbits. Methods: Hepatic artery embolization was carried out in fifteen New Zealand rabbits by using Lutrol® F 127 as embolic material. The rabbits were followed up for 4 weeks. Examinations, including liver function, CT scanning and angiography were regularly conducted. Every three rabbits were sacrificed immediately after the procedure and each time at 3 days, 1, 2 and 4 weeks after the procedure. The specimens were collected and sent for histopathologic examination. Results: After the operation, a transient elevation of ALT and AST level was observed in all rabbits, which reached its peak at the fifth day and turned to its initial level in two weeks. The complete occlusion of segmental artery branches and distal branches was achieved immediately after the embolization and no recanalization was detected on DSA performed 4 weeks after the operation. The liquefaction necrosis of liver parenchyma was demonstrated on CT scanning performed 1 to 2 weeks after the treatment, and punctate necrosis foci were still seen in 4 weeks. The CT value of the high-density lesions located within the embolized region gradually decreased with the time. This changing process of CT value spread from lesion's center to lesion's margin and lasted for 4 weeks. Pathological examination conducted immediately after the embolization showed that the tiny hepatic arteries were completely filled with Lutrol® F 127, and no embolic material could be found in hepatic sinusoids, portal veins or pulmonary arteries. At the 3th day and 1st, 2nd, 4th week, Lutrol® F 127 together with thrombosis was found in pre-sinusoidal arterioles and meanwhile complete disappearance of hepatic lobules with hyperplasia of interlobular connective tissue could also be seen. Conclusion: In the environment of body temperature, the thermosensitive and slow

  18. Experimental observation of lens damage after low doses of γ-ray irradiation to rabbit eyes

    International Nuclear Information System (INIS)

    Objective: To investigate and evaluate low dose γ-ray radiation induced lens damage. Methods: Both eyes of each rabbit were exposed to a single dose of 25 or 50 cGy γ-rays in two groups, respectively. Samples were examined by transmission electron microscopy (TEM) and slit lamp microscopy (SLM)after irradiation. Results: Three days after 25 and 50 cGy irradiation,the epithelial cells of lens equator al region showed marked swelling and many vacuoles formed in intercellular space and cytoplasm,and accompanied by increased multi-lamellar bodies. Five months after irradiation, SLM of both groups showed that the posterior sub-capsule cortex exhibited clusters of vacuoles; 11 months after 50 cGy irradiation,the posterior sub-capsule and deep cortex manifested marked cloudy opacities. Conclusion: Low doses of γ-ray (25 and 50 cGy) irradiation can markedly damage lens of rabbits

  19. Olfactory route for cerebrospinal fluid drainage into the cervical lymphatic system in a rabbit experimental model☆

    OpenAIRE

    Liu, Haisheng; Ni, Zhili; Chen, Yetao; Wang, Dong; Qi, Yan; Zhang, Qiuhang; Wang, Shijie

    2012-01-01

    The present study analyzed the anatomical association between intracranial subarachnoid space and the cervical lymphatic system. X-ray contrast medium and Microfil® (Microfil compounds fill and opacify microvascular and other spaces of non-surviving animals and post-mortem tissue under physiological injection pressure) were injected into the cisterna magna of the rabbit, and perineural routes of cerebrospinal fluid outflow into the lymphatic system were visualized. Under a surgical operating ...

  20. Circular Herpesvirus sylvilagus DNA in spleen cells of experimentally infected cottontail rabbits.

    OpenAIRE

    Medveczky, P; Kramp, W J; Sullivan, J L

    1984-01-01

    Cottontail rabbits (Sylvilagus floridanus) were infected with Herpesvirus sylvilagus, and spleen cells were analyzed for the presence of virus-specific, covalently closed circular, and linear DNA molecules by a simple electrophoretic technique, followed by transfer to nitrocellulose filters and hybridization with cloned viral DNA (Gardella et al., J. Virol. 50:248-254, 1984). Approximately 0.2 copies per cell of circular DNA and 0.2 copies per cell of linear DNA were detected by hybridization...

  1. STRUCTURAL FEATURES OF RABBIT SHIN-BONE AFTER FLUOROSIS EXPERIMENTAL MODEL CREATION

    Directory of Open Access Journals (Sweden)

    V.N. Karmazov

    2009-03-01

    Full Text Available Impact of ecological factors and chronic intoxications on an organism is not accounted in treatment of bone injuries. The more significant osseous tissue modifications arise in case of fluoric intoxication accompanied by structural bone modifications. The main structural changes of more and less variability by mineral density of similar shin-bone zones of rabbit at fluorosis have been revealed in the research.

  2. Experimental observation of human bone marrow mesenchymal stem cell transplantation into rabbit intervertebral discs

    Science.gov (United States)

    Tao, Hao; Lin, Yazhou; Zhang, Guoqing; Gu, Rui; Chen, Bohua

    2016-01-01

    Allogeneic bone marrow mesenchymal stem cell (BMSC) transplantation has been investigated worldwide. However, few reports have addressed the survival status of human BMSCs in the intervertebral discs (IVDs) in vivo following transplantation. The current study aimed to observe the survival status of human BMSCs in rabbit IVDs. The IVDs of 15 New Zealand white rabbits were divided into three groups: Punctured blank control group (L1-2); punctured physiological saline control group (L2-3); and punctured human BMSCs transfected with green fluorescent protein (GFP) group (L3-4, L4-5 and L5-6). One, 2, 4, 6 and 8 weeks after transplantation the IVDs were removed and a fluorescence microscope was used to observe the density of GFP-positive human BMSCs. The results indicated that in the sections of specimens removed at 1, 2, 4, 6 and 8 weeks post-transplantation, no GFP-positive cells were observed in the control groups, whereas GFP-positive cells were apparent in the nucleus pulposus at all periods in the GFP-labeled human BMSCs group, and the cell density at 6 and 8 weeks was significantly less than that at 1, 2 and 4 weeks post-transplantation (P<0.001). Thus, it was identified that human BMSCs were able to survive in the rabbit IVDs for 8 weeks.

  3. The influence of atorvastatin on tendon healing: an experimental study on rabbits.

    Science.gov (United States)

    Esenkaya, Irfan; Sakarya, Bulent; Unay, Koray; Elmali, Nurzat; Aydin, Nasuhi Engin

    2010-06-01

    Hyperlipidemia is a major risk factor for coronary heart disease. The most commonly used antihyperlipidemic drugs are 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase inhibitors (statins), of which atorvastatin is one of the most widely used. Little is known about the relationship between tendinopathy and HMG CoA reductase inhibitors (statins) or the effects of atorvastatin use on tendon healing following surgical repair of tendon rupture. We hypothesized that atorvastatin negatively affects this healing process. The Achilles tendons of 16 New Zealand rabbits were ruptured surgically and repaired with sutures. Eight of the rabbits were given oral atorvastatin. The other 8 served as a surgical control group. Six weeks postoperatively, all the rabbits were sacrificed, and the repaired tendons were removed. After standard histological preparation, fibroblastic activity, re-vascularization, collagenization, collagen construction, and inflammatory-cell infiltration were evaluated. On comparing the atorvastatin and surgical control groups, we observed no difference in fibroblastic activity. Although it did not reach statistical significance in our study, a difference was noted in revascularization, collagenization, and inflammatory cell infiltration; and a statistical difference was observed in collagen construction. Doubt remains about the adverse effect of atorvastatin use during tendon healing. Further investigations in animal and human models are needed on the effects of tendon healing when atorvastatin is administered for a longer time frame prior to the injury. PMID:20806777

  4. The expression of calpain-I in cerebral vasospasm:a preliminary investigation in experimental rabbits

    International Nuclear Information System (INIS)

    Objective: To investigate the expression of calpain-I in rabbit model with cerebral vasospasm and to discuss the clinical significance of calpain-I in cerebral vasospasm. Methods: Twenty-five healthy New Zealand white rabbits were randomly divided into two groups: normal control group (n = 5) and subarachnoid hemorrhage (SAH) group (n = 20). The subarachnoid hemorrhage was created by endovascular punctures. The SAH group, according to the time of sacrifice, was randomly and equally subdivided into 12-hour, 1-day, 3-day, and 7-day subgroups with 5 rabbits in each group. The expressions of calpain-I of the smooth muscles of basilar arteries in both groups were determined by means of immunohistochemistry technique, and the semi-quantitative analysis of calpain-I was evaluated by histochemistry score. Results: The expression of calpain-I was slightly elevated at 12 hours after SAH, it reached to its peak at the third day, and then declined on the seventh day. The semi-quantitative analysis of calpain-I showed that the difference in the expression of calpain-I between two groups was of statistic significance (P < 0.01). Conclusion: After subarachnoid hemorrhage, the expression of calpain-I in the spasmodic smooth muscles of basilar artery is enhanced, and the changes of the expression level is early, gradual and continuous. Most probably, the calpain-I participates in the process of vessel wall structural signal transduction change in cerebral vasospasm. (authors)

  5. STUDY ON THE THERAPEUTIC EFFECT AND MECHANISMS OF AURICULAR ACUPUNCTURE FOR TREATMENT OF EXPERIMENTAL DIABETES MELLITUS IN THE RABBIT

    Institute of Scientific and Technical Information of China (English)

    MA Ruiling; YUAN Qing; WANG Guobin

    2002-01-01

    In the present study,fasting blood glucose (FBG)concentration and serum insulin concentration and chenges of the ultramicrostructure of the pancreatic islet cells are used as the indexes to evaluate the therapeutic effect of aurioular acupuncture in the treatment of experimental diabetes mellitus(DM)and to analyze ist mechanisms in the rabbit .Thirty-two rabbits are randomly divided into control,model,acupuncture and medication groups with 8 cases being in each group.DM model is established by intravenous injection of 4% Alloxan (150mg/kg).Otopoints used are low-resistanoa points of Yidan(MA-SC 6),Neifenmi (MA-IC 3), Jiaogan(MA-AH 7), Fei (MA-IC 1), Wei (MA-IC),Shen (MA-SC),etc..Inmedication group, the animals are fed with glbenzcyclamied, ( 1.25 mg/kg/day). The treatment of both groups is given once daily, 20 sessions altogether. Results display that after 2 courses of treatment (20 sessions), in comparison with pre-treatment and model group, FBG contents in acupuncture and medication groupsd decreased significantly (P<0.01), while insulin contents of acupuncture and medication groups increaesd remarkably (P<0.05). No significant differences between acupuncture and medication groups in FBG and insulin contents. Accordingly, electron microscopic dbservation shows some improvements in partial organells as Iysosome being fewer,reduction of the nucleolar swelling, etc. in both acupucture group and medication group. It shows that restoration of the structure of islet β-cells of the pancreas, increase of the synthesis and release of insulin as well as the activity of insulin mayy contribute to the effect of auricular acupuncture in lowring blood glucose level in DM rabbits.

  6. Assessment of tissue viability after frostbite injury by technetium-99m-sestamibi scintigraphy in an experimental rabbit model

    International Nuclear Information System (INIS)

    Frostbite causes injury to the tissue by direct ice-crystal formation at the cellular level with cellular dehydration and microvascular occlusion. Muscle that initially appears viable on reperfusion may subsequently become necrotic because of microcirculatory collapse. Since muscle is a sensitive tissue in frostbite injury, we used technetium-99m-sestamibi limb scintigraphy to assess tissue viability in an experimental rabbit model. Twelve rabbits were used for this investigation. The right hind limb of the rabbits was immersed to the ankle joint in a container filled with 90% ethanol at -25 C for 10 min. Frostbitten limbs were allowed to thaw in air at room temperature. Imaging and pathological examination of the affected limbs were performed 2 h, 24 h, 48 h and 72 h after freezing. In 2-h images, initial hypoperfusion was seen that corresponded to circulatory collapse. In 24-h images, there was hyperperfusion (so-called period of temporary reperfusion), corresponding to circulatory restoration. In 48-h images, a second hypoperfusion corresponded to viable but ischaemic tissue. In 72-h images, there was non-perfusion of the limb that correlated with the pathologically determined diagnosis of necrosis. All scintigraphic patterns correlated with pathological findings. We suggest that these scintigraphic patterns in soft tissue may be helpful in distinguishing between frank infarction and reversible ischemia and therefore may be useful in selecting early therapeutic or surgical interventions to salvage bone and soft tissue. Further studies are needed to show the usefulness of 99mTc sestamibi scintigraphy in clinical frostbite cases. (orig.)

  7. Assessment of tissue viability after frostbite injury by technetium-99m-sestamibi scintigraphy in an experimental rabbit model

    Energy Technology Data Exchange (ETDEWEB)

    Sarikaya, I. [Dept. of Nuclear Medicine, Kocaeli University Medical Faculty, Kocaeli (Turkey); Cemal Aygit, A. [Department of Plastic and Reconstructive Surgery, Trakya University Medical Faculty, Edirne (Turkey); Candan, L. [Department of Pathology, Trakya University Medical Faculty, Edirne (Turkey); Sarikaya, A.; Berkarda, S. [Dept. of Nuclear Medicine, Trakya University Medical Faculty, Edirne (Turkey); Tuerkyilmaz, M. [Dept. of Chemistry, Trakya University Faculty of Science, Edirne (Turkey)

    2000-01-01

    Frostbite causes injury to the tissue by direct ice-crystal formation at the cellular level with cellular dehydration and microvascular occlusion. Muscle that initially appears viable on reperfusion may subsequently become necrotic because of microcirculatory collapse. Since muscle is a sensitive tissue in frostbite injury, we used technetium-99m-sestamibi limb scintigraphy to assess tissue viability in an experimental rabbit model. Twelve rabbits were used for this investigation. The right hind limb of the rabbits was immersed to the ankle joint in a container filled with 90% ethanol at -25 C for 10 min. Frostbitten limbs were allowed to thaw in air at room temperature. Imaging and pathological examination of the affected limbs were performed 2 h, 24 h, 48 h and 72 h after freezing. In 2-h images, initial hypoperfusion was seen that corresponded to circulatory collapse. In 24-h images, there was hyperperfusion (so-called period of temporary reperfusion), corresponding to circulatory restoration. In 48-h images, a second hypoperfusion corresponded to viable but ischaemic tissue. In 72-h images, there was non-perfusion of the limb that correlated with the pathologically determined diagnosis of necrosis. All scintigraphic patterns correlated with pathological findings. We suggest that these scintigraphic patterns in soft tissue may be helpful in distinguishing between frank infarction and reversible ischemia and therefore may be useful in selecting early therapeutic or surgical interventions to salvage bone and soft tissue. Further studies are needed to show the usefulness of {sup 99m}Tc sestamibi scintigraphy in clinical frostbite cases. (orig.)

  8. Degradable Gelatin Microspheres as an Embolic Agent: an Experimental Study in a Rabbit Renal Model

    Energy Technology Data Exchange (ETDEWEB)

    Ohta, Shinichi; Nitta, Norihisa; Takahashi, Masashi; Murata, Kiyoshi [Shiga University of Medical Science, Shiga (Japan); Tabata, Yasuhiko [2Institute for Frontier Medical Sciences, Kyoto University, Kyoto (Japan)

    2007-10-15

    To investigate the basic characteristics of degradable gelatin microspheres (GMSs), including their embolic behavior and degradation periods when they are used as embolic materials in the renal arteries of rabbit models. Based on the GMS particle size, 24 kidneys were divided into 3 groups of eight kidneys, and each group was embolized with a different GMS particle size (group 1: 35-100 {mu}m, group 2: 100-200 {mu}m, and group 3: 200-300 {mu}m). From each group, two rabbits were sacrificed immediately after embolization (day 0), and a pair of rabbits from each group underwent an angiogram and were sacrificed on days 3, 7, and 14, respectively, after embolization. The level of arterial occlusion, the pathological changes in the renal parenchyma, and the degradation of the GMSs were evaluated angiographically and histologically. A follow-up angiogram on days 0, 3, 7, and 14 revealed the presence of wedge-shaped poorly-enhanced areas in the parenchymal phase as seen in all groups. The size of these areas tended to increase with the particle diameter, and persisted up to day 14. On days 3, 7, and 14, parenchymal infarctions were observed histologically in all cases, and this observation corresponded with the parenchyma being supplied by the embolized arteries. GMSs of group 1 mainly reached the interlobular arteries, while those of group 3 mainly reached the interlobar arteries. In all but two cases, the GMSs were identified histologically even on day 14, and sequential degradation was histologically identified in all GMS groups. GMSs can be used as degradable embolic materials which can control the level of embolization.

  9. Experimental study of interventional infusion thermochemotherapy in rabbit liver VX2 tumor

    International Nuclear Information System (INIS)

    Objective: Effectiveness of two kinds of thermochemotherapy infusion from intraarterial approach were studied in the grafted liver VX2 tumors of rabbit. Methods: VX2 tumor model was established in 30 Newzland rabbit's livers. Percutaneous transfemoral hepatic arterial catheterization with fixation of the catheter tip inside the feeding vessel was carried out under DSA guidance. All 30 rabbits were divided into three groups (n=10 in each group), normal temperature 100 ml saline + Adriamycin (ADM) infusion (group 1), 60 degree C 100 ml saline + ADM continuous perfusion (group 2) and 60 degree C 100 ml saline + ADM intermittent perfusion (group 3). After the perfusion, the lasting time periods of 43-45 degree C for tumor tissue of group 2 and 3 together with the concentrations of ADM within tumor's tissue were measured. Results: Concentrations of ADM were shown as (12.013 ± 2.237) μg/ml, (17.622 ± 1.368) μg/ml, and (11.519 ± 1.225) μg/ml for group 2, group 3 and group 1 respectively. 60 degree C intermittent perfusion vs 60 degree C continuous perfusion showed P0.05. 43-45 degree C period lasting time (min) for 60 degree C continuous perfusion vs 60 degree C intermittent perfusion were (4.1 ± 2.7) min and (11.3 ± 3.3) min respectively, the latter was three times more than the former. There were no differences shown between the temperature, respiration and heart rate of group 2 and group 3. Conclusion: Intermittent intraarterial perfusion thermochemocherapy is a more effective interventional management among all thermochemotherapies. (authors)

  10. Experimental research of prednisolone in new zealand white rabbits with radiation induced lung injury

    International Nuclear Information System (INIS)

    Objective: To observe the preventive and therapeutic effect of Prednisolone in radiation injury of lungs. Methods: 45 male New Zealand white rabbits were randomly divided into three groups with 15 in each; the blank group (a), the irradiation group (b) and the group with irradiation and Prednisolone (c). Group (a) has no other treatment. Group (b) was given injections Sodium Chloride on the first day of irradiation, with 0.1 ml/kg/d for 4 weeks. Group (c) was given intraperitoneal injections of Prednisolone on the first day of irradiation, with 0.4 mg/kg/d for 4 weeks. CT scanning was performs before irradiation and the 1, 3, 5 months after the irradiation. The animals were killed by cutting off the neck after 1, 3, 5 months of radiation. The right lungs were removed to give HE staining and immunohistochemical staining for the histological evaluation. Results: No significant changes were found in group a in CT scanning. The pathological changes in group c is less than serious than those in group b. Group c is less serious than in pathological changes those in group b. Immunohistochemical results; One, three, and five months after irradiation, the number of positive cells were highest in group b, and was significantly higher in group c than in group a. Conclusion: High dose irradiation of the lung of New Zealand rabbit tissue can successfully abtain the established radiation-induced lung injury animal models. Prednisolon can reduce the radiation-induced lung injury in rabbits New Zealand, and has a certain preventive effect. (authors)

  11. Circular Herpesvirus sylvilagus DNA in spleen cells of experimentally infected cottontail rabbits.

    Science.gov (United States)

    Medveczky, P; Kramp, W J; Sullivan, J L

    1984-11-01

    Cottontail rabbits (Sylvilagus floridanus) were infected with Herpesvirus sylvilagus, and spleen cells were analyzed for the presence of virus-specific, covalently closed circular, and linear DNA molecules by a simple electrophoretic technique, followed by transfer to nitrocellulose filters and hybridization with cloned viral DNA (Gardella et al., J. Virol. 50:248-254, 1984). Approximately 0.2 copies per cell of circular DNA and 0.2 copies per cell of linear DNA were detected by hybridization with a cloned viral DNA fragment. The size of the viral DNA was estimated at ca. 158 kilobase pairs. Restriction endonuclease patterns suggested structural similarities to cottontail herpesvirus DNA. PMID:6092696

  12. Influence of experimental influenza infection of the eye on the course of herpetic keratitis in rabbits.

    Science.gov (United States)

    Zaitseva, N S; Vinogradova, V L; Krichevskaya, G I

    1978-11-01

    Influenza virus monoinfection and combined influenza and herpes simplex virus (HSV) infection of the eye was studied in rabbits. Influenza A/Hong Kong/1/68 (H3N2) virus caused a clinically overt disease of the eye only after inoculation into the eye chamber. The combined influenza and HSV infection of the eye induced severe iridocyclitis. The combined infection of the eye with influenza virus and HSV had a more severe course than HSV monoinfection. The occurence of influenza antibody in the lacrimal fluid in the presence of the antigen in eye tissues is of diagnostic importance in determining the aetiology of the disease. PMID:35945

  13. Experimental study of the effect of platelet-rich plasma on osteogenesis in rabbit

    Institute of Scientific and Technical Information of China (English)

    张长青; 袁霆; 曾炳芳

    2004-01-01

    @@ Platelet-rich plasma (PRP) is produced from a patient's own blood by centrifugation, and PRP contains several kinds of growth factors in high concentration such as platelet derived growth factor (PDGF), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), and so on.1 These growth factors have proved to offer an improved quality and speed of healing for both hard and soft tissue.2 In this study, PRP compounded with porous bioceramic was used to repair a bone defect in rabbit radius. The radiographic and histological qualitative and quantitative observations were performed to evaluate osteogenesis.

  14. Activity of aminoacyl-tRNA synthetases in experimental hyperthyroidism in muscle tissue of the rabbit

    International Nuclear Information System (INIS)

    In cardial and femoral muscles of rabbits specific activities of aminoacyl-tRNA synthetases for twenty amino acids were generally similar, namely the activities towards amino acids and their amides, leucine, isoleucine, histidine, tyrosine, proline and serine were considerably lower than towards the remaining amino acids. Specific activities of most aminoacyl-tRNA synthetases were higher in hyperthyroidism than in euthyreosis, and were higher in femoral muscle than in heart. The response to thyroxine treatment of individual aminoacyl-tRNA synthetases in both kinds of muscles varied with respect to most of the amino acids. (author). 22 refs, 1 fig., 1 tab

  15. Study of blood count in the validity experimental appendicitis in rabbits ESTUDO DO HEMOGRAMA NA VIGÊNCIA DE APENDICITE EXPERIMENTAL EM COELHOS

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    Eduardo Chibeni Fernandes Ramos

    1999-04-01

    Full Text Available A group of thirty six rabbits was studied, with weight ranging from 2400 to 3100 grams, in order to evaluate the blood test on the validity of experimental acute appendicitis. In order to do that, the animals were distributed amony three groups of 12 rabbits each, that corresponded to observation periods of 24 hours(Group A, 48 hours(Group B and 72 hours(Group C. The presence of macroscopic appendicitis was verified in all the rabbits of the experiment on the three studied periods. The blood test study showed statistic significance when reading the values of haemoglobin, haematocrit and monocytes in Group C; of total and segmented leucocytes, eosinophiles, limphocytes and monocytes in Group B; when the groups faced each other, however, there was no estatistic significance. So, it was concluded that the values of haemoglobin, red blood, white cells and haematocrit are not suitable markers for experimental appendicitis in rabbits.A comparação de resultados de exames laboratoriais de coelhos hígidos e coelhos com apendicite provocada, tem como objetivo procurar um marcador para o diagnóstico desta patologia. Foram estudados 36 coelhos com peso corporal variando de 2400 a 3100 gramas, distribuído em três grupos de 12 coelhos, que correspondem aos períodos de observação de 24 horas(Grupo A, 48 horas(Grupo B e 72 horas(Grupo C. Verificou-se a presença de apendicite macroscópica em todos os coelhos do experimento, nos três grupos estudados. O estudo dos hemogramas mostrou alterações nas leituras dos valores de leucócitos totais, segmentados, eosinófilos, linfócitos e monócitos no Grupo B; da hemoglobina, hematócrito e monócitos no Grupo C; no entanto, quando se confrontam os resultados entre os grupos não há significância estatística. Conclui-se que os valores da hemoglobina, glóbulos vermelhos, glóbulos brancos e hematócrito não são marcadores adequados para apendicite experimental em coelhos.

  16. Experimental intra-arterial administration of SR-2508 in the rabbit V2 carcinoma model

    International Nuclear Information System (INIS)

    The use of the nitroimidazole radiation sensitizers, misonidazole and SR-2508, has been limited by a variety of side-effects, principally peripheral neuropathies. To increase the effectiveness of radiosensitization and reduce the administered dose of the sensitizers, intra-arterial delivery of SR-2508 was performed and followed with 10Gy of 4 MeV photon beam radiotherapy in the rabbit V2 carcinoma tumour model. Comparison was made with rabbits treated with an identical dose of SR-2508 given intravenously prior to photon beam radiotherapy. Control animals were treated with radiation therapy only, intra-arterial SR-2508 only, or had no treatment. Neither radiation therapy alone nor SR-2508 alone had any effect on tumour growth. In the intra-arterial group, tumour growth was slower and size was significantly smaller than all other groups. Results suggest that the intra-arterial infusion of radiation sensitizer produces greater tumour response than intravenous infusion when each is combined with radiation therapy. (author)

  17. Experimental intra-arterial administration of SR-2508 in the rabbit V2 carcinoma model

    Energy Technology Data Exchange (ETDEWEB)

    Coldwell, D.M.; Huff, J.; Marcellus, H.; Coldwell, S.G.

    1989-03-01

    The use of the nitroimidazole radiation sensitizers, misonidazole and SR-2508, has been limited by a variety of side-effects, principally peripheral neuropathies. To increase the effectiveness of radiosensitization and reduce the administered dose of the sensitizers, intra-arterial delivery of SR-2508 was performed and followed with 10Gy of 4 MeV photon beam radiotherapy in the rabbit V2 carcinoma tumour model. Comparison was made with rabbits treated with an identical dose of SR-2508 given intravenously prior to photon beam radiotherapy. Control animals were treated with radiation therapy only, intra-arterial SR-2508 only, or had no treatment. Neither radiation therapy alone nor SR-2508 alone had any effect on tumour growth. In the intra-arterial group, tumour growth was slower and size was significantly smaller than all other groups. Results suggest that the intra-arterial infusion of radiation sensitizer produces greater tumour response than intravenous infusion when each is combined with radiation therapy.

  18. Experimental study on therapy of ultraviolet blood irradiation and oxygenation in acute soman intoxication in rabbits

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xian-qing; MU Shi-jie; ZHANG Xiao-di; CHEN Rui; XIA Ai-jun; LIANG Xin; HAI Chun-xu

    2005-01-01

    Objective: To study the therapy effect of ultraviolet blood irradiation and oxygenation(UBIO) on blood AChe activity and lung injury due to acute soman intoxication in rabbits. Methods :Forty rabbits were randomly divided into 4 groups: normal control group, intoxication group, routine therapy group and UBIO therapy group. Blood AChe activity and artery blood gas were analyzed 2 h after intoxication. ACP and AKP activities in BALF were determined respectively. Results:Blood AChe activity in intoxication group was lower than that in normal control group (P<0. 05). BALF ACP and AKP activities in intoxication group were higher than that in normal control group. Blood AChe activities in UBIO therapy group increased and were higher than that in intoxication and routine therapy groups. Compared with intoxication group, BALF ACP and AKP activities were decreased (P<0.05) in UBIO therapy group,while artery blood pH, PaO2 and SaO2 increased (P<0.05). Conclusion: UBIO therapy can elevate blood AChe activity and alleviate lung injury induced by soman intoxication. So it may be a new way to treat acute soman intoxication.

  19. Adipose derived stromal vascular fraction improves early tendon healing: an experimental study in rabbits

    Directory of Open Access Journals (Sweden)

    Mehdi Behfar

    2011-11-01

    Full Text Available Tendon never restores the complete biological and mechanical properties after healing. Bone marrow and recently adipose tissue have been used as the sources of mesenchymal stem cells, which have been proven to enhance tendon healing. Stromal vascular fraction (SVF, derived from adipose tissue by an enzymatic digestion, represents an alternative source of multipotent cells, which undergo differentiation into multiple lineages to be used in regenerative medicine. In the present study, we investigated potentials of this source on tendon healing. Twenty rabbits were divided into control and treatment groups. Five rabbits were used as donors of adipose tissue. The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon. Immediately after suture repair, either fresh stromal vascular fraction from enzymatic digestion of adipose tissue or placebo was intratendinously injected into the suture site in treatments and controls, respectively. Cast immobilization was continued for two weeks after surgery. Animals were sacrificed at the third week and tendons underwent histological, immunohistochemical, and mechanical evaluations. By histology, improved fibrillar organization and remodeling of neotendon were observed in treatment group. Immunohistochemistry revealed an insignificant increase in collagen type III and I expression in treatments over controls. Mechanical testing showed significant increase in maximum load and energy absorption in SVF treated tendons. The present study showed that intratendinous injection of uncultured adipose derived stromal vascular fraction improved structural and mechanical properties of repaired tendon and it could be an effective modality for treating tendon laceration.

  20. An experimental study of gallbladder sclerosis with 10% phenol in rabbits

    International Nuclear Information System (INIS)

    To evaluate a new reliable sclerosant of the gallbladder, we attempted gallbladder ablation with 10% phenol, and the results compared with those from using 95% ethanol which had been used previous as gallbladder sclerosing agent in laboratory animals in other reports. After laparotomy, ligation of the cystic ducts with silk and cannulation of gallbladder with 18 gauge angiocatheter were done. Then, transcatheter administration of two different sclerosing agents was performed in 8 rabbits respectively and normal saline in four rabbits as a control. Additionally, preliminary washing with each agent were implement to prevent the dilutional effect of residual bile and bleeding. All animals survived without complication. Eight animals were used for each agent, four each being sacrificed two weeks and six weeks after administration of sclerosing agents respectively. In our results, 10% phenol was more effective than 95% ethanol in denuding the gallbladder epithelium and promoting fibrosis of gallbladder wall. And it was relatively safe in regard to the dilutional effect of residual fluid and bleeding during procedure. Toxic effects on the liver evaluated by examination of histologic specimen were non-specific except for edematous swelling on some case, which had also been observed on others including control group. 10% phenol can be considered to be a promising sclerosant for gallbladder ablation, but further study of its toxicity is needed before its application on human gallbladder

  1. The Healing Effects of Autologous Mucosal Grafts in Experimentally Injured Rabbit Maxillary Sinuses

    Science.gov (United States)

    Topdag, Murat; Kara, Ahmet; Konuk, Esma; Demir, Necdet; Ozturk, Murat; Calıskan, Sebla; Topdag, Deniz Ozlem; Ulubil, Arif; Keskin, Ibrahim Gurkan; Iseri, Mete

    2016-01-01

    Objectives Healing processes of the nose and paranasal sinuses are quite complex, and poorly understood. In this study, we aimed to compare the effect of mucosal autologous grafts on the degenerated rabbit maxillary sinus mucosa with spontaneous wound healing. It is hypothesized that mucosal grafts will enhance ciliogenesis and improve the morphology of regenerated cilia. Methods Ten female New Zealand rabbits were included in the study. They underwent external maxillary sinus surgery through a transcutaneous approach. A total of 20 maxillary sinuses were randomly divided into 2 groups: ‘spontaneous healing group’ and ‘autologous graft group.’ The animals were sacrificed at the 14th day after the surgery. Scanning electron microscope (SEM), and light microscope were used for the evaluation. Results Cellular composition of the graft group is better than the spontaneous healing group. The graft group had larger areas covered with ciliary epithelium than the spontaneous healing group, and the mean length of the cilias were also longer. Additionally, there were wider cilia with abnormal morphology areas in the spontaneous healing group. Conclusion In our opinion, covering of the denuded areas with a graft improves re-epithelization, and may prevent the early complications after sinus surgeries. PMID:26976026

  2. EXPERIMENTAL TRIALS OF LIVE ATTENUATED AND INACTIVATED STAPHYLOCOCCUS AUREUS VACCINES IN RABBITS

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    A. SHAKOOR, M. ATHAR, G. MUHAMMAD, S. U. RAHMAN1, A. A. BUTT2, I. HUSSAIN 2 AND R. AHMAD3

    2006-04-01

    Full Text Available This study was conducted as a preliminary step on the rabbits for comparative efficacy of different vaccines of Staphylococcus aureus. Typical alpha-beta Staph. aureus species from a clinically affected mastitic buffalo was isolated. After proper identification based on cultural and morphological characteristics and API-Staph Trac system, a selected Staph. aureus isolate was used to prepare four different mastitis vaccines (Bacterin, oil-adjuvanted, dextran sulphate adjuvanted and live attenuated after confirmation for pathogenicity and antigenicity, followed by its safety and sterility evaluation. Vaccines were tried in 25 rabbits divided into 5 equal groups. A separate vaccine was administered s/c @ 0.2 ml per animal and boosted at 15 days later. It was found that IHA antibody titers were higher (GMT 32-128 in live attenuated, dextran sulphate adjuvanted (GMT 32-128 and oil-adjuvanted (GMT 16-64 than the bacterin treated (GMT 16-32 group. All the vaccines showed an apparent immune response than the unvaccinated control group.

  3. Apoptotic imaging of experimental hypoxic ischemic brain injury in neonatal rabbits

    International Nuclear Information System (INIS)

    Objective: Hypoxic ischemic brain injury(HIBD) is a severe abnormality of new-born babies causing disability in their future lives. Early detection of HIBD is thereafter of great clinical significance. The current study was to investigate the value of apoptotic imaging with 99Tcm-hydrazinonicotinamide (HYNIC) Annexin V in detecting HIBD in a neonatal rabbit model. Methods: Twenty-one baby rabbits (8-10 d after birth) were modeled after Rice's method and divided into the sham, HIBD 4 h and HIBD 40 h groups. Planar imaging at different time points[60 min (sham, HIBD 4 h); 5, 30, 60, 120 min (HIBD 40 h)] after injection of 99Tcm-Annexin V were carried out and paraffin sections were performed after sacrifice of the animals when imaging completed. The ratio of counts or pixel at injury side to the opposite side (T/NT) was calculated. Routine MRI and diffusion weighted imaging (DWI) were performed in HIBD 4 h group and shame group. Results: The 60 min T/NT ratios from the sham, HIBD 4 h and HIBD 40 h groups were 0.94 ± 0.14, 1.32 ± 0.11 and 1.81 ± 0.07, respectively(F=82.385, P99Tcm-HYNIC-Annexin V, which appears to be of clinical potential in neonatal ischemic-hypoxic brain injury. (authors)

  4. Experimental study on the mechanism of intrapulmonary kinetics of 123I-IMP in balloon occluded bronchus of rabbit

    International Nuclear Information System (INIS)

    To investigate the mechanism of intrapulmonary kinetics of N-isopropyl-p-123I-iodoamphetamine (123I-IMP), we performed lung scanning with 123I-IMP in 13 rabbits in which a unilateral bronchus was occluded by a balloon catheter. In 9 rabbits, on the delayed distribution images, an abnormal accumulation of 123I-IMP was detected in the hypoperfusion area of the peripheral lung field of the occluded bronchus, which was detected on lung perfusion images with 99mTc-MAA. Comparison of early (a summation image during 20 seconds immediately after injection) and delayed (a summation image from 70 to 90 minutes after injection) distribution images confirmed that during this period, 123I-IMP accumulated in the area of reduced distribution of 123I-IMP. Moreover, at the site of abnormal accumulation, washout of 123I-IMP from the lung was delayed compared with that in the normal lung field. We find this experimental study is similar to the results in 63 clinical cases, which we have reported previously, and confirm that pulmonary arterial perfusion, especially hypoperfusion, influences the intrapulmonary kinetics of 123I-IMP. (author)

  5. MR staging of malignant musculoskeletal tumors: An experimental study on MR and pathologic correlation of rabbit VX-2 carcinoma

    International Nuclear Information System (INIS)

    To evaluate the reliability of MR imaging in tissue characterization and depiction of tumor boundaries, we performed MR pathologic correlation using parosteally implanted VX-2 carcinoma in 17 rabbit thighs. T1-weighted, T2-weighted and Gd-DTPA enhanced T1-weighted axial images were obtained 10-30 days after tumor implantation. After the animals were killed, frozen and sectioned along the MR imaging planes, and histopathologic examination were done. For accurate MR pathologic correlation, rabbit were fixed on the cardboard plate to minimize position change during the procedure. Tumor boundaries depicted on MR images were larger than those depicted on the specimen. Small tumors were surrounded by capsule-like loose connective tissue. Loose connective tissue became compact with tumor growth. This connective tissue showed high signal intensity on both T2-weighted and Gd-DTPA enhanced T1-weighted images. Muscle atrophy with fatty tissue accumulation around the tumor also contributed to the high signal intensity on MR images. Peritumoral edema and inflammatory reaction were not remarkable. Six of 8 cases with bone marrow fibrosis were detected on MR images. We concluded that peritumoral loose connective tissue and muscle atrophy exaggerated the size of experimentally induced malignant musculoskeletal tumors on MR images

  6. Differential effect of Pistacia vera extracts on experimental atherosclerosis in the rabbit animal model: an experimental study

    Directory of Open Access Journals (Sweden)

    Halabalaki Maria

    2010-07-01

    Full Text Available Abstract Background Lipid-enriched diets and oxidative stress are risk factors for the development of atherosclerosis. The effects of the methanolic (ME and cyclohexane (CHE extracts of the Pistacia vera nut, often included in the Mediterranean diet, were studied in the rabbit model of atherosclerosis. Methods and results Twenty-four New Zealand White rabbits received atherogenic diet (Control Group, supplemented with ME (Group ME or CHE (Group CHE for 3 months. Previously, a GC-MS and a UHPLC LC-DAD-ESI(--HRMS/MS method were developed to investigate the extracts' chemical profiles. Blood samples at baseline and monthly determined lipid profile, lipid peroxidation and liver function. The aorta, myocardium and liver were examined histologically at 3 months. Groups ME and CHE had significantly higher HDL- and non-significantly lower LDL-cholesterol median % changes from baseline than the Control Group. Triacylglycerol was significantly higher in Group CHE vs. Control. MDA values were significantly lower in Group ME vs. Control and CHE. ALT and AST were significantly higher in Group CHE vs. Control. γ-GT was lower in Group ME vs. Control. Aortic intimal thickness was significantly less in Groups ME and CHE vs. Control; Group ME atherosclerotic lesions were significantly less extensive vs. Groups Control and CHE. Only Group CHE had significant liver fatty infiltration. Conclusions During short-term administration concomitantly with atherogenic diet, both P. vera extracts were beneficial on HDL-, LDL-cholesterol and aortic intimal thickness. The ME additionally presented an antioxidant effect and significant decrease of aortic surface lesions. These results indicate that P. vera dietary inclusion, in particular its ME, is potentially beneficial in atherosclerosis management.

  7. Radiographic changes in cortical bone after intramedullary nailing. An experimental investigation on rabbits

    Energy Technology Data Exchange (ETDEWEB)

    Kaartinen, E.; Paavolainen, P.; Silvennoinen, T.; Slaetis, P.; Karaharju, E.

    Transverse osteotomies of the midshaft of the tibia in 75 rabbits were stabilized with intramedullary nails which prevented rotational instability. In a further 36 animals intramedullary nailing was performed without preceding osteotomy. The animals were able to move freely immediately after the operation without any supporting cast or splint. The structural changes in the bones were analysed radiographically 3 to 24 weeks after the operation. Fracture repair was accompanied by moderate periosteal callus formation and remodelling of the callus was seen from 9 weeks on. A sleeve of new endosteal bone surrounding the nail was found after 12 weeks. Porotic transformation of the diaphyseal cortical bone was seen in both osteotomized and non-osteotomized bones from 12 weeks onwards. It is concluded that intramedullary nailing provides favorable conditions for fracture repair and enables accurate radiographic assessment of the healing. Attention is focused on the causes and features of secondary porotic transformation of the cortical bone after intramedullary nailing. (orig.).

  8. The impact of nicotine on osseointegration. An experimental study in the femur and tibia of rabbits

    DEFF Research Database (Denmark)

    Balatsouka, Dimitra; Gotfredsen, Klaus; Lindh, Christian H;

    2005-01-01

    2 months. The pump delivered 6 mug/kg/min of nicotine for the animals in the test group. Blood was withdrawn and plasma cotinine levels were measured weekly. Thirty-two titanium implants were inserted into the femur and tibia of all rabbits after 4 weeks and after 6 weeks of nicotine....../placebo exposure. Thus, 2- and 4-week healing groups were created. Biomechanical evaluation by (i) resonance frequency analysis test (RFA) on all implants after insertion and before sacrifice and (ii) removal torque test (RMT) on tibial implants before sacrifice was performed. All implants placed in the femur were...... processed to undecalcified ground sections. The percentage of bone-to-implant contact (BIC) and of bone area within the threads (BD-i) were measured. RESULTS: No significant difference in RMT and RFA values was found between the test and the control group. Histomorphometric measurements of the BIC and the...

  9. Experimental radiation synovectomy in rabbit knee with holmium-166 ferric hydroxide macroaggregate

    International Nuclear Information System (INIS)

    Holmium-166 ferric hydroxide macroaggregate (Ho-166 FHMA) particles possess two important properties for radiosynovectomy; relatively short half-life of the radioisotope and appropriate carrier size. Both these minimize radioactive leakage from the treated joint. This study was conducted to assess the effects of Ho-166 FHMA on synovium and synovial fluid in rabbit knee joints. Whole-knee autoradiography was utilized to determine distribution of radioactivity after intra-articular Ho-166 FHMA injection. Intra-articular injection of Ho-166 FHMA resulted in focal acute radiation necrosis in synovial lining but no hyperplasia of synoviocytes. Later, subsynovial fibrosis became evident. White blood cell and total protein levels in the joint fluid were elevated because of intra-articular inflammation due to the acute effects of radiation. Whole knee autoradiograms showed uneven distribution of the radionuclide along the synovium and extraarticular leakage on the third day after treatment

  10. Use of human nail for reconstruction of the orbital floor: an experimental study in rabbits.

    Science.gov (United States)

    Görgülü, Tahsin; Akçal, Arzu; Uğurlu, Kemal

    2016-07-01

    The orbital floor is the thinnest part of the orbital wall, and in 20% of all maxillofacial injuries it is fractured. Autografts, allografts, and alloplastic materials are used in reconstruction, but there is no consensus about which material is the most appropriate. Nail is a semirigid material that is easy to reshape and is not antigenic. Alloplastic materials, which are used in reconstructions of the orbital floor, have various complications and are expensive. Autografts have donor-site problems, high rates of resorption, and take a long time to do. We created bilateral 10mm defects in the orbital floors in 18 New Zealand rabbits. We reconstructed the left orbital floors with double-ground human nail while the right orbital floors were left open as controls. The orbital floors were examined macroscopically and microscopically at 4, 8, and 12 weeks postoperatively, and there were no macroscopic signs of infection, inflammation, or extrusion. Forced duction tests showed that it was possible to induce movement of the eyeball for all 18 of the reconstructed sides throughout the observation period, and in 14 of the 18 rabbits on the control sides. Positive forced duction test shows us that orbital muscles are trapped in orbital floor defect and due to this movement of eyeball is restricted. Acute and chronic inflammation, fibrosis, vascularisation, and the presence of foreign body giant cells were evaluated microscopically. Acute inflammation and the presence of foreign body giant cells were recorded as mild, whereas fibrosis, chronic inflammation, and vascularisation were severe, as were epithelialisation on the maxillary sinus side of the nails, calcification, and progression of collagen. We found no signs of resorption of the nails. PMID:27090026

  11. The Efficiency of Vascular Embolization Using Alginate Gel : An Experimental Study in Rabbit

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Woo Baek; Kang, Yeong Han [Dept. of Diagnostic Radiology, Daegu Catholic University Hospital, Daegu (Korea, Republic of); Kim, Jong Ki [Dept. of Biomedical Engineering, Daegu Catholic University, Daegu (Korea, Republic of)

    2009-03-15

    The purpose of this study was to investigate the applicability of poly-L-guluronic alginate (PGA) gel in vascular embolization with angiography simulation. To prepare a gel-forming PGA from no guluronate-rich Laminaria japonica, a new acid hydrolysis method was employed with a lower HCL concentration (0.03 M) and a shorter treatment time (5 min). The obtained PGAs were selected based on gel stability and viscosity. Glass aneurysm model was used to simulate gel embolization in vitro. Then, finally, the PGA was used to embolize the renal vascular system by using a rabbit model and angiography. Glass aneurysm model was made to simulate gel embolization procedure. PGA solution was injected from pump through 2-way catheter. Subsequent injection of CaCl{sub 2} successfully formed gels inside aneurysm model that conforming to its inner contour. In rabbit model, first, renal artery and aorta leading to the right kidney were ligated to block blood flow, then conventional contrast agent was injected through aorta to check the arterial patency to the left kidney. In sequential artery injection method, PGA and CaCl{sub 2} were injected through renal artery sequentially via a single catheter. Re-injection of contrast agent after removing ligated aorta showed blood flow to the right kidney but no flow in the left kidney. This result demonstrated a complete blocking of blood flow due to gel formation in vascular bed of the left kidney. Instillation of calcium alginate into aneurysm model and arterial system in vivo produced an embolization that better fills and conforms to the contour of aneurysms or blocking vascular bed completely. Therefore, PGA was effective endovascular occlusion materials and provide an efficiency of vascular angiography.

  12. The Efficiency of Vascular Embolization Using Alginate Gel : An Experimental Study in Rabbit

    International Nuclear Information System (INIS)

    The purpose of this study was to investigate the applicability of poly-L-guluronic alginate (PGA) gel in vascular embolization with angiography simulation. To prepare a gel-forming PGA from no guluronate-rich Laminaria japonica, a new acid hydrolysis method was employed with a lower HCL concentration (0.03 M) and a shorter treatment time (5 min). The obtained PGAs were selected based on gel stability and viscosity. Glass aneurysm model was used to simulate gel embolization in vitro. Then, finally, the PGA was used to embolize the renal vascular system by using a rabbit model and angiography. Glass aneurysm model was made to simulate gel embolization procedure. PGA solution was injected from pump through 2-way catheter. Subsequent injection of CaCl2 successfully formed gels inside aneurysm model that conforming to its inner contour. In rabbit model, first, renal artery and aorta leading to the right kidney were ligated to block blood flow, then conventional contrast agent was injected through aorta to check the arterial patency to the left kidney. In sequential artery injection method, PGA and CaCl2 were injected through renal artery sequentially via a single catheter. Re-injection of contrast agent after removing ligated aorta showed blood flow to the right kidney but no flow in the left kidney. This result demonstrated a complete blocking of blood flow due to gel formation in vascular bed of the left kidney. Instillation of calcium alginate into aneurysm model and arterial system in vivo produced an embolization that better fills and conforms to the contour of aneurysms or blocking vascular bed completely. Therefore, PGA was effective endovascular occlusion materials and provide an efficiency of vascular angiography.

  13. Transplantation of autologous bone marrow stem cells via hepatic artery for the treatment of acute hepatic injury: an experimental study in rabbits

    International Nuclear Information System (INIS)

    Objective: To evaluate the transplantation of autologous bone marrow stem cells via hepatic artery in treating acute hepatic injury in experimental rabbit models and to clarify the synergistic effect of hepatocyte growth-promoting factor (pHGF) in stem cell transplantation therapy for liver injury. Methods Acute hepatic injury models were established in 15 experimental rabbits by daily subcutaneous injection of CCl4 olive oil solution with the dose of 0.8 ml/kg for 4 days in succession. The experimental rabbits were randomly and equally divided into three groups: study group A (stem cell transplant, n = 5), study group B (stem cell transplant + pFHG, n = 5), and control group (n = 5). Bone marrow of 5 ml was drawn from the tibia in all rabbits of both study groups, from which bone marrow stem cells were isolated by using density gradient centrifugation, and 5 ml cellular suspension was prepared. Under fluoroscopic guidance, catheterization through the femoral artery was performed and the cellular suspension was infused into the liver via the hepatic artery. Only injection of saline was carried out in the rabbits of control group. For the rabbits in group B, pFHG (2.0 mg/kg) was administered intravenously every other day for 20 days. At 2, 4 and 8 weeks after stem cell transplantation, hepatic function was determined. Eight weeks after the transplantation all the rabbits were sacrificed and the liver specimens were collected and sent for pathological examination. Results After stem cell transplantation, the hepatic function was gradually improved.Eight weeks after the transplantation, the activity of AST, ALT and the content of ALB, TBIL were significantly lower than that before the procedure, while the content of GOLB was markedly increased in all rabbits. In addition, the difference in the above parameters between three groups was statistically significant (P < 0.05). Pathologically, the hepatocyte degeneration and the fiberous hyperplasia in the study groups were

  14. An experimental study on angiogenesis in rabbit VX2 brain tumor using perfusion CT

    International Nuclear Information System (INIS)

    Objective: To validate the perfusion CT method for the reflection of angiogenesis in VX2 rabbit brain tumors, and to correlate CT findings with MVD and VEGF. Methods: VX2 rabbit brain tumor model was established by injection of viable tumor cells (107/ml) through a 5 mm-hole to the right of the sagittal suture and 5 mm posterior to the coronal suture bored by dental drill. MRI was performed every 2 days after seven days of implantation to evaluate the growth of the tumor. 20 New Zealand white rabbits with tumor size over 3 mm in diameter were randomly divided into 2 groups according to the tumor growth time with those less than 3 weeks as group 1 and those more than 3 weeks as group 2, and perfusion CT were performed accordingly. CT measurements of BV, BF and PS from tumor, peritumor and contralateral normal tissue regions were obtained. After that the animals were sacrificed and 2% Evans blue (2 ml/kg) was given intravenously in 16 of these animals 1 hour prior to sacrifice to detect breakdown of the blood brain barrier. VEGF and MVD were evaluated in immunohistochemical examination of the specimens. Results: Tumor had significantly higher BV [(13.25±4.58) ml·100 g-1], BF[(166.14±69.62) ml·100 g-1·min-1], and PS(8.01 ml·min-1·100 g-1) than peritumor[(2.38±0.80)ml·100 g-1, (62.49±25.83)ml· 100 g-1·min-1 and 0.03 ml·min-1·100 g-1] and normal tissue region [(2.24±0.75)ml·100 g-1, (55.72±21.24)ml·100 g-1·min-1, and 0.04 ml·min-1·100 g-l] (P=0.000). Tumor BV [(16.41±4.12)ml·100 g-1], BF[(208.77±63.00)ml·100 g-1·min-1], and MVD (61.20± 12.93)/high power field in group 2 were significantly higher than those [(10.09±2.27) ml·100 g-l, (123.51±47.18)ml·100 g-1·min-l, and (41.40±7.34)/high power field] in group 1 (Ps=0.861, P=0.000). Conclusion: Perfusion CT can distinguish tumor from peritumor and normal tissue clearly, reflect tumor angiogenesis accurately, and provide useful information for the evaluation of brain tumor. (authors)

  15. Evaluation of the toxicity of onyx compared with n-butyl 2-cyanoacrylate in the subarachnoid space of a rabbit model: an experimental research

    International Nuclear Information System (INIS)

    The toxic effects of onyx, its solvent dimethyl sulphoxide (DMSO), and n-butyl 2-cyanoacrylate (NBCA) were evaluated after infusion into the subaracnoid space of a rabbit model. Each of the two various concentrations of onyx, pure DMSO, NBCA, and normal saline solution were percutaneously infused into the pontocerebellar cisternae of 39 domestic male albino rabbits, after which, the brain stems and medial cerebellar tissues were harvested for biochemical and histopathological studies. The specimens infused in various concentration of onyx, DMSO, and NBCA showed neural tissue necrosis and edema with inflammatory cell infiltration in the acute stage. Although the mean values of the lipid peroxidase in the control, saline, and NBCA groups were found to be almost similar, they were found to be low in the onyx and DMSO groups. This experimental study suggests that NBCA, and various concentrations of onyx and DMSO have toxic effects on the neural tissues of rabbits when infused into the subarachnoid space. (orig.)

  16. Evaluation of the toxicity of onyx compared with n-butyl 2-cyanoacrylate in the subarachnoid space of a rabbit model: an experimental research

    Energy Technology Data Exchange (ETDEWEB)

    Bakar, Bulent [Kirikkale University, School of Medicine, Department of Neurosurgery, Kirikkale (Turkey); Kirikkale University Faculty of Medicine, Department of Neurosurgery, Kirikkale (Turkey); Oruckaptan, Hakan H.; Hazer, Burcu D. [Hacettepe University, School of Medicine, Department of Neurosurgery, Ankara (Turkey); Saatci, Isil [Hacettepe University, School of Medicine, Department of Radiology, Ankara (Turkey); Atilla, Pergin; Muftuoglu, Sevda F. [Hacettepe University, School of Medicine, Department of Histology and Embriology, Ankara (Turkey); Kilic, Kamer [Hacettepe University, School of Medicine, Department of Biochemistry, Ankara (Turkey)

    2010-02-15

    The toxic effects of onyx, its solvent dimethyl sulphoxide (DMSO), and n-butyl 2-cyanoacrylate (NBCA) were evaluated after infusion into the subaracnoid space of a rabbit model. Each of the two various concentrations of onyx, pure DMSO, NBCA, and normal saline solution were percutaneously infused into the pontocerebellar cisternae of 39 domestic male albino rabbits, after which, the brain stems and medial cerebellar tissues were harvested for biochemical and histopathological studies. The specimens infused in various concentration of onyx, DMSO, and NBCA showed neural tissue necrosis and edema with inflammatory cell infiltration in the acute stage. Although the mean values of the lipid peroxidase in the control, saline, and NBCA groups were found to be almost similar, they were found to be low in the onyx and DMSO groups. This experimental study suggests that NBCA, and various concentrations of onyx and DMSO have toxic effects on the neural tissues of rabbits when infused into the subarachnoid space. (orig.)

  17. Experimental study on intra-arterial infusion of basic fibroblast growth factor in the ischemic limbs of rabbit model

    International Nuclear Information System (INIS)

    Objective: To evaluate the effect of intra-arterial infusion of basic fibroblast growth factor (bFGF) on improving neovascularization, vascular perfusion and the function of partially ischemic limbs of rabbits. Methods: Twenty-seven New Zealand male rabbits were selected. Partial ischemia model was induced by surgical ligation of the primary branches of right femoral artery in each animal, and the left hind limb of each animal was served as a nonischemic control. Then, 27 rabbits were randomly assigned to three groups: intra-arterial (IA) infusion of bFGF (n=9), intravenous (IV) infusion of bFGF and IA infusion of saline (n=9). Infusion was separately performed immediately after vascular ligation, 8th and 15th days post-surgery with 10 μg (4 ml) of bFGF per-time (or the same volume of saline). The differences between three groups and between ischemic and nonischemic limbs of the same group were compared and evaluated by the following indexes: (1) vessel section count (VSC), vessel section surface area (VSS) and vessel section perimeter (VSP) in the field of ischemic muscle tissues taken at 22nd day postoperatively; (2) capillary refilling time of ischemic limbs; and (3) functional and trophic changes of ischemic limbs. Statistical differences were evaluated by one-way ANOVA and T test. Results: VSC, VSS and VSP of the IA-bFGF group were significantly increased than those of the IV-bFGF and IA-saline groups (P<0.01). At 22nd day postoperatively, the capillary refilling time, new hair growth, the appearance and function of all ischemic limbs in IA-bFGF group were approximately normal. However, in IA-saline group, the ischemic changes, capillary refilling time and the function of ischemic limbs were not improved significantly. All the indexes of IV-bFGF group showed no difference statistically from those of IA-saline group. Conclusions: This experimental study identifies that intra-arterial infusion of bFGF may significantly promote neovascularization and vascular

  18. Olfactory route for cerebrospinal fluid drainage into the cervical lymphatic system in a rabbit experimental model

    Institute of Scientific and Technical Information of China (English)

    Haisheng Liu; Zhili Ni; Yetao Chen; Dong Wang; Yan Qi; Qiuhang Zhang; Shijie Wang

    2012-01-01

    The present study analyzed the anatomical association between intracranial subarachnoid space and the cervical lymphatic system. X-ray contrast medium and Microfil(R) (Microfil compounds fill and opacify microvascular and other spaces of non-surviving animals and post-mortem tissue under physiological injection pressure) were injected into the cisterna magna of the rabbit, and perineural routes of cerebrospinal fluid outflow into the lymphatic system were visualized. Under a surgical operating microscope, Microfil was found within the subarachnoid space and along the olfactory nerves. At the nasal mucosa, a lymphatic network was identified near the olfactory nerves, which crossed the nasopharyngeal region and finally emptied into the superficial and deep cervical lymph nodes. Under a light microscope, Microfil was visible around the olfactory nerves and within lymphatic vessels. These results suggested that cerebrospinal fluid drained from the subarachnoid space along the olfactory nerves to nasal lymphatic vessels, which in turn, emptied into the cervical lymph nodes. This anatomical route, therefore, allowed connection between the central nervous system and the lymphatic system.

  19. An experimental study on transcatheter embolization with mixture of ethanolamine oleate and microfibrillar collagen in rabbits

    International Nuclear Information System (INIS)

    To evaluate ethanolamine oleate (EAO)-microfibrillar collagen (MFG) mixture as a new scleroembolic material for the interventions requiring both permanent obliteration of vascular lumen and atrophy of mass, such as for the facial AVM and other hypervascular soft tissue masses. Twenty-nine transcatheter transarterial embolization of renal arteries were performed in six groups of rabbits classified by the EAO concentration and the addition of MFC. Post embolization angiography, gross morphological and microscopic examinations of embolized kidneys were performed immediately, 3 days, 2 weeks and 4 weeks after embolization. Analysing points were the usefulness as a scleroembolic material (endovascular retention, thrombogenic-sclerosing effect, perivascular fibrosis and inflammatory reaction), effects of the EAO concentration and the addition of MFC. EAO-MFC mixture satisfied all ideal conditions of scleroembolic agent; persistent endovascular retention, good thrombogenic-sclerosing effect with a mild inflammatory reaction and significant atrophy of kidney. The effect of increasing concentration of EAO was proximal embolization. The effects of MFC were promotion of proximal embolization, endovascular retention and sclerosing effect. EAO-MFC mixture can be used as a new effective scleroembolic material for the various hemodynamic situations in which embolic level can be controlled by EAO concentration and the addition of MFC

  20. Experimental cloning of embryos through human-rabbit inter-species nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    JI Jingjuan; GUO Tonghang; TONG Xianhong; LUO Lihua; ZHOU Guixiang; FU Yingyun; LIU Yusheng

    2007-01-01

    Therapeutic cloning,which is based on human somatic cell nuclear transfer,is one of our major research objectives.Though inter-species nuclear transfer has been introduced to construct human somatic cell cloned embryos,the effects of type,passage,and preparation method of donor cells on embryo development remain unclear.In our experiment,cloned embryos were reconstructed with different passage and preparation methods of ossocartilaginous cell,skin fibroblast,and cumulus cells.The cumulus cell embryos showed significantly higher development rates than the other two (P<0.05).The development rate of embryos reconstructed with skin fibroblasts of different passage number and somatic cells of different chilling durations showed no significant difference.Also,fluorescence in situ hybridization (FISH)was conducted to detect nuclear derivation of the embryos.The result showed that the nuclei of the inter-species cloned embryo cells came from human.We conclude that (1)cloned embryos can be constructed through human-rabbit interspecies nuclear transfer;(2)different kinds of somatic cells result in different efficiency of nuclear transfer,while in vitro passage of the donor does not influence embryo development;(3)refrigeration is a convenient and efficient donor cell preparation method.Finally,it is feasible to detect DNA gcnotype through FISH.

  1. Anchorage of titanium implants with different surface characteristics: an experimental study in rabbits

    DEFF Research Database (Denmark)

    Gotfredsen, K; Berglundh, T; Lindhe, J

    2000-01-01

    ) TiO2-blasted with particles of grain size 10 to 53 microns; (3) TiO2-blasted, grain size 63 to 90 microns; (4) TiO2-blasted, grain size 90 to 125 microns; (5) titanium plasma-sprayed (TPS). The surface topography was determined by the use of an optical instrument. Twelve rabbits, divided into two...... and Sy values, whereas the highest scores were recorded for the TPS implants. The corresponding Sa and Sy values for the TiO2-blasted implants were higher when a larger size of grain particles had been used for blasting. At all four observation intervals, the TPS implants had the highest and the...... turned implants the lowest RMT scores. The differences between the various TiO2-blasted implants were, in general, small, but the screws with the largest Sa value had higher RMT scores at 6, 9, and 12 weeks than implants with lower Sa values. The histologic analysis of the sections representing 6, 9, and...

  2. The Effect of Irreversible Electroporation on the Femur: Experimental Study in a Rabbit Model.

    Science.gov (United States)

    Song, Yue; Zheng, Jingjing; Yan, Mingwei; Ding, Weidong; Xu, Kui; Fan, Qingyu; Li, Zhao

    2015-01-01

    Irreversible electroporation (IRE) is a novel ablation method that has been tested in humans with lung, prostate, kidney, liver, lymph node and presacral cancers. As a new non-thermal treatment, the use of IRE to ablate tumors in the musculoskeletal system might reduce the incidence of fractures. We aimed to determine the ablation threshold of cortical bone and to evaluate the medium- and long-term healing process and mechanical properties of the femur in a rabbit model post-IRE ablation. The ablation threshold of cortical bone was between 1090 V/cm and 1310 V/cm (120 pulses). IRE-ablated femurs displayed no detectable fracture but did exhibit signs of recovery, including osteoblast regeneration, angiogenesis and bone remodeling. In the ablation area, revascularization appeared at 4 weeks post-IRE. Osteogenic activity peaked 8 weeks post-IRE and remained high at 12 weeks. The mechanical strength decreased briefly 4 weeks post-IRE but returned to normal levels within 8 weeks. Our experiment revealed that IRE ablation preserved the structural integrity of the bone cortex, and the ablated bone was able to regenerate rapidly. IRE may hold unique promise for in situ bone tissue ablation because rapid revascularization and active osteogenesis in the IRE ablation area are possible. PMID:26655843

  3. Dynamic contrast-enhanced MRI for the detection of testicular ischemia in experimental induced testicular torsion of rabbits

    International Nuclear Information System (INIS)

    Objective: To evaluate the dynamic contrast-enhanced MRI (DCE-MRI) in detecting experimentally induced testicular ischemia. Methods: Thirty healthy male New Zealand rabbits were randomly assigned into 6 groups. There were 5 rabbits in each of the following experimental groups: (1) Normal control, (2) Sham-operated, (3) ischemia of 3 h group, (4) ischemia of 6 h group, (5) ischemia of 12 h group, (6) ischemia of 24 h group. In all experiment groups, the right testis served as the internal control while the left testis served as the experimental side. DCE-MRI for each animal lasts about 10 minutes. Signal enhanced ratios (SERs) of ROI for both sides of each group were calculated by a computer, and parameters of SERs of 30 s, 75 s, 120 s and maximal SER were used for statistical analysis. Time intensity curves (TICs) were made for two sides of each group via Excel 2003 software and classified into 4 types. Statistical analysis was performed to compare the differences of SERs between left and right testis by two independent Kolmogorov-Smirnov test. Results: In group 1 and 2, significant enhancement was observed on both testes of 10 rabbits. The enhancement decreased gradually with the elongation of ischemia in torsion groups. Three cases of type Ⅰ and 2 cases of type Ⅱ were observed in group 1, 5 cases of type Ⅰ in group two, 2 cases of type Ⅰ and 3 cases of type Ⅱ b in group three, 2 cases of type Ⅰ and 2 cases of type Ⅱ b in group four, 5 cases of type b in group five and 5 cases of type Ⅲ in group six were noticed in the left testes. And in TICs of right testes, all cases showed TICs of type Ⅰ except 2 cases of type Ⅱ a in group six. In four torsion groups, the values for SER75 of the left side were 0.084%, 0.076%, 0.164% and 0.065%, while the right side were 0.255%, 0.410%, 0.586% and 0.302% (P120 in group three, five and six were 0.221%, 0.158% and 0.059% for the left side, and 0.405%, 0.522% and 0.207% for the right side (P<0.05). The values

  4. Endothelial Dysfunction in Experimental Atherosclerosis in the Rabbit with Extraction of Instantaneous Changes in the Arterial Wall

    Directory of Open Access Journals (Sweden)

    Tavoos Rahmani-Cherati

    2015-10-01

    Full Text Available Background: In this study, we used a new computerized analytical method for the measurement of the endothelial function in sequential ultrasound images and compared it with histological studies, using the abdominal aorta in normal and atherosclerotic rabbits.Methods: Six rabbits received a standard rabbit chow as the normal group and the other 6 rabbits were fed a high cholesterol diet for four weeks as the atherosclerotic group. B-mode images of the abdominal aorta with 46 frames per second were saved over three cardiac cycles at baseline and during acetylcholine or nitroglycerin drug infusion in the normal and atherosclerotic rabbits. In order to evaluate endothelial-dependent relaxation, acetylcholine-mediated dilation (AMD was measured during the infusion of acetylcholine at a rate of 0.5 µg/kg/min and endothelial-independent relaxation was evaluated by measuring nitroglycerine-mediated dilation (NMD during the infusion of nitroglycerin at a rate of 5 µg/kg/min. In addition, the ultrasonic evaluation was confirmed by histopathological evaluation of the abdominal aorta.Results: Significant differences in AMD were detected between the normal and the four-week cholesterol-fed rabbits (p value < 0.05, whereas there were no significant differences in NMD between the two groups (p value > 0.05. No microscopic intimal lesions were seen in the normal rabbits, but intimal thickening was observed in the histological studies in the four- week cholesterol-fed rabbits. Additionally, the total cholesterol, triglycerides, low-density lipoprotein cholesterol, and high- density lipoprotein cholesterol levels were remarkably increased in the sera of the four-week cholesterol-fed rabbits (p value< 0.05.Conclusion: A new automatic method can help accurately evaluate the endothelial function in normal and hypercholesterolemic rabbits.

  5. Experimental study of treatment on rabbit liver VX2 tumor by selective hepatic artery embolization hyperthermia with nano superparamagnetic iodized oil

    International Nuclear Information System (INIS)

    Objective: To observe the therapeutic response of liver tumors by arterial embolization hyperthermia with Nano Superparamagnetic Iodized Oil (NSIO) using rabbit VX2 liver tumor model. Methods: A total 24 rabbits containing experimental hepatic tumors were randomly assigned to one of four groups as follows: NSIO embolization hyperthermia group (group A), Lipidol embolization group (group B), NSIO embolization group (group C), and control group (group D), each groups contain 6 VX2 rabbits. Fourteen days after implantation of the experimental hepatic tumor, VX2 rabbits were treated. In group A group B and group C, the rabbits hepatic proper artery were selectively catheterized by 3 Fr microcatheters via right femoral artery under fluoroscopic guidance. 10% NSIO 0.5 ml (group A and group C) or Lipidol 0.5 ml(group B) infused into proper hepatic artery. Three days after embolization, the rabbits in group A and group B were exposed to gap-type alternating magnetic field for 30 minutes, while rabbits in group C and group D have not been exposed to alternating magnetic field. The liver tumor size were measured by CT scanning before and 14 days after treatment then the animals were sacrificed, the liver, lung, heart spleen and kidney were harvested for histopathology examination, the liver tumor size were detected directly. Results: All subjects experienced uneventful 14 days survivals, on the biochemical examination, there were no changes about the function of liver and renal in each group 14 days after treatment compare to pre- treatment. Fourteen days after treatment, the tumor size decreased by 8.09% in group A, but increased by 9.72% and 13.00% (P<0.05) in group B and group C respectively, in group D, the tumor size increased by 57.50% (P<0.01). In histopathology examination, the tumor necrosis in three treatment groups were manifest, particular in group A. Conclusion: Arterial embolization hyperthermia with NSIO has obvious therapeutic response to experimental hepatic

  6. Protein C activation during the initial phase of experimental acute pancreatitis in the rabbit

    DEFF Research Database (Denmark)

    Ottesen, L H; Bladbjerg, E-M; Osman, M;

    2000-01-01

    BACKGROUND: Disturbances of coagulation and fibrinolysis are well-known systemic effects of acute necrotising pancreatitis (ANP). The purpose of this experimental study was to evaluate the initial events in the haemostatic activation during ANP in an animal model with relevance to the human...

  7. Isolation of Trichophyton mentogrophytes var mentogrophytes from naturally infected laboratory albino rats: experimental infection and treatment in rabbits

    Directory of Open Access Journals (Sweden)

    N. A. Issa

    2009-01-01

    Full Text Available The present study demonstrated for the first time the occurrence of dermatophytosis in naturally infected rats and from asymptomatic and from breeding boxes of white rats kept in animal housing of college of Veterinary Medicine, University of Dohuk, Iraq. The prevalence rate of infection was (28%, clinically infected rats characterized by appearance of scaly ovoid type lesions with crusty edge and patch of hair loss mostly seen on the back, neck and face of the infected rats, itching was reported in some rats. Only one species of the trichophyton, T. mentogrophytes var mentogrophytes was isolated with growth rate (85.71% of samples collected from clinically infected rats, and (28.57% from asymptomatic and from breeding cages, the growth was observed within the 21 days at 25ºC on Sabouraud's Dextrose Agar. Lacto phenol cotton blue staining slides of T. mentogrophytes var mentogrophytes revealed both microconidia and macroconidia. Microconidia found in numerous numbers often in dense cluster which were hyaline, smooth walled and predominantly spherical to sub spherical in shape, varying numbers of chlamydoconidia. Spiral hyphae and smooth, thin walled clavate shaped multicelled macroconidia were also present. The study also dealt with experimental infection in rabbits with T. mentogrophytes var mentogrophytes and treated by two drugs, natural herbal preparation of acidic pomegranate (Punica granatum fruit and synthetic nystatine ointment. The complete recovery of lesions was recorded after 14 days and 21 days of topical application of a pomegranate and nystatine ointment for 5 successive days respectively.

  8. Histologic and histomorphometric evaluation of the effect of lactoferrin combined with anorganic bovine bone on healing of experimentally induced bony defects on rabbit calvaria

    OpenAIRE

    Mojgan Paknejad; Amir Reza Rokn; Ali Akbar Sabur Yaraghi; Flora Elhami; Mohammad Javad Kharazifard; Neda Moslemi

    2012-01-01

    Background: Recent studies have shown that lactoferrin promotes the proliferation and differentiation of osteoblasts and inhibits osteoclast-mediated bone resorption. Anorganic bovine bone (ABB) graft has been extensively used as an osteoconductive material in the bone reconstructive surgeries. The purpose of this study was to examine whether the combination of lactoferrin with Bio-Oss would improve ossification in experimentally induced bone defects in rabbit calvaria. Materials and Meth...

  9. Histocompatibility and Long-Term Results of the Follicular Unit-Like Wigs after Xenogeneic Hair Transplantation: An Experimental Study in Rabbits

    OpenAIRE

    Yu Sun; Feng Lu; Ge Liu; Zhi-Dan Zhang; Zijie Zhang; Zhi-Qi Hu

    2011-01-01

    Objective. This study was designed to observe the histocompatibility and long-term results of wigs after xenogeneic hair transplantation and to explore the possibility of industrial products in clinical application. Methods. The human hair and melted medical polypropylene were preceded into the follicular unit-like wigs according to the natural follicular unit by extrusion molding. 12 New Zealand rabbits were used as experimental animals for wigs transplantation. The histocompatibility of pol...

  10. Keratinized versus non-keratinized preputial flap onlay urethroplasty: does it make any difference in the histological analysis? An experimental study in rabbits

    Directory of Open Access Journals (Sweden)

    Marnio Costa

    2013-12-01

    Full Text Available Purpose To compare the histological characteristics of keratinized versus non-keratinized onlay island flaps in an experimental rabbit model. Materials and Methods Sixteen male rabbits were randomly allocated into two experimental groups: keratinized and non-keratinized onlay island flaps. A defect was created in the ventral aspect of the penile urethra. In the keratinized group, a longitudinal island flap was harvested from the external prepuce and rotated to cover the urethral defect. In the non-keratinized group a transverse island flap was harvested from the inner prepuce. The animals were sacrificed after 2, 4, 8 and 12 weeks. Results The flaps were viable in all animals, and no deaths were associated with the procedure. Two urethrocutaneous fistulas were identified, one in each experimental group. A similar pattern of fibrosis was identified in both groups. The keratinized epithelium of the external prepuce kept its histological aspect and keratin production. Both keratinized and non-keratinized groups presented a slight decrease on the epithelial thickness, however without a statistically significant difference between groups. Conclusions In this short-term rabbit model, we observed that the stratified squamous keratinized epithelium from the external prepuce kept its keratin production. There was no statistical influence of the flap type on the mean epithelial thickness.

  11. Nonalcoholic steatohepatitis as a novel player in metabolic syndrome-induced erectile dysfunction: an experimental study in the rabbit.

    Science.gov (United States)

    Vignozzi, Linda; Filippi, Sandra; Comeglio, Paolo; Cellai, Ilaria; Sarchielli, Erica; Morelli, Annamaria; Rastrelli, Giulia; Maneschi, Elena; Galli, Andrea; Vannelli, Gabriella Barbara; Saad, Farid; Mannucci, Edoardo; Adorini, Luciano; Maggi, Mario

    2014-03-25

    A pathogenic link between erectile dysfunction (ED) and metabolic syndrome (MetS) is now well established. Nonalcoholic steatohepatitis (NASH), the hepatic hallmark of MetS, is regarded as an active player in the pathogenesis of MetS-associated cardiovascular disease (CVD). This study was aimed at evaluating the relationship between MetS-induced NASH and penile dysfunction. We used a non-genomic, high fat diet (HFD)-induced, rabbit model of MetS, and treated HFD rabbits with testosterone (T), with the selective farnesoid X receptor (FXR) agonist obeticholic acid (OCA), or with the anti-TNFα mAb infliximab. Rabbits fed a regular diet were used as controls. Liver histomorphological and gene expression analysis demonstrated NASH in HFD rabbits. Several genes related to inflammation (including TNFα), activation of stellate cells, fibrosis, and lipid metabolism parameters were negatively associated to maximal acetylcholine (Ach)-induced relaxation in penis. When all these putative liver determinants of penile Ach responsiveness were tested as covariates in a multivariate model, only the association between hepatic TNFα expression and Ach response was confirmed. Accordingly, circulating levels of TNFα were increased 15-fold in HFD rabbits. T and OCA dosing in HFD rabbits both reduced TNFα liver expression and plasma levels, with a parallel increase of penile eNOS expression and responsiveness to Ach. Also neutralization of TNFα with infliximab treatment fully normalized HFD-induced hypo-responsiveness to Ach, as well as responsiveness to vardenafil, a phosphodiesterase type 5 inhibitor. Thus, MetS-induced NASH in HFD rabbits plays an active role in the pathogenesis of ED, likely through TNFα, as indicated by treatments reducing liver and circulating TNFα levels (T or OCA), or neutralizing TNFα action (infliximab), which significantly improve penile responsiveness to Ach in HFD rabbits. PMID:24486698

  12. Preventive effects of atorvastatin on atherosclerosis in experimental rabbits are associated with reduction in cholesterol level and VCAM-1 expression

    OpenAIRE

    Yu, Lei; Ling, Qin; Rui, Qu; Jiaying, Zhang

    2011-01-01

    In this study, blood samples of rabbits were collected to measure plasma total cholesterol, total triglyceride, and LDL-cholesterol. After 16 weeks of high cholesterol diet with or without atorvastatin treatment, the rabbits were sacrificed and morphological changes in tissues were examined with hematoxylin and eosin (HE) staining, and the expression of vascular-cell adhesion molecule 1 (VCAM-1) was determined by immunostaining and reverse transcription-polymerase chain reaction (RT-PCR). We ...

  13. Disposable rabbit

    Science.gov (United States)

    Lewis, Leroy C.; Trammell, David R.

    1986-01-01

    A disposable rabbit for transferring radioactive samples in a pneumatic transfer system comprises aerated plastic shaped in such a manner as to hold a radioactive sample and aerated such that dissolution of the rabbit in a solvent followed by evaporation of the solid yields solid waste material having a volume significantly smaller than the original volume of the rabbit.

  14. Plasma rico em plaquetas de coelhos: introdução a um modelo animal experimental Platelet-rich plasma in rabbits: introduction of one experimental animal model

    Directory of Open Access Journals (Sweden)

    Marco Antonio de Oliveira-Filho

    2008-12-01

    Full Text Available RACIONAL: Muitas dúvidas ainda permanecem no que se refere às ações dos fatores de crescimento e do plasma rico em plaquetas sobre o mecanismo de reparação tissular. Há necessidade de serem esclarecidos pontos controversos ainda existentes. OBJETIVO: Obter o plasma rico em plaquetas em coelhos através de um método simplificado e ao mesmo tempo adequado, introduzindo um modelo experimental que possa ser utilizado em estudos posteriores. MÉTODOS: Foram utilizados 25 coelhas da raça Nova Zelândia e sem doenças prévias. Quinze mL de sangue de cada animal foi coletado, sendo 10 mL submetidos à dupla centrifugação. Para comprovar a efetividade do método proposto realizou-se contagem mecânica do sangue, bem como do produto final. RESULTADO: Obteve-se uma concentração média de plaquetas no plasma rico em plaquetas 687% maior que a contagem inicial observada no sangue venoso periférico. Para as variáveis: contagem inicial de plaquetas, contagem de plaquetas no plasma rico em plaquetas e enriquecimento, foram obtidos os limites de 95% de confiança para suas médias, sendo que, no que se refere ao percentual de enriquecimento, existe 95% de chance de que o intervalo de (530-844 contenha a média real de enriquecimento de plaquetas. CONCLUSÃO: O método simplificado utilizado permite a obtenção de plasma rico em plaquetas adequado permitindo seu uso em estudos dos fatores de crescimento nos mecanismos de reparação tecidual.BACKGROUND: Multiple uncertainties still exist about the action of the growth factors and the platelet-rich plasma on the mechanism of repair. AIM: To obtain the platelet-rich plasma in rabbits through a simplified and suitable method, creating an experimental model. METHODS: Twenty-five female New Zealand rabbits without previous diseases were used. Fifteen mL of blood of each rabbit was collected and 10 mL of the collected blood were twice centrifugated. To check the effectiveness of the proposed method

  15. In-vitro effects of PDE5 inhibitor and statin treatment on the contractile responses of experimental MetS rabbit's cavernous smooth muscle

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    Yasin Erden

    2014-03-01

    Full Text Available Objective: Hypercholesterolaemia promotes erectile dysfunction through increased superoxide formation and decreased nitric oxide bioactivity in cavernosal tissue. The role of nitric oxide on erectile function is well known. Statins have lipid lowering properties and can modulate endothelial nitric oxide bioavailability. Sildenafil, enhances smooth muscle relaxation in corpus cavernosum. We invastigated in-vitro effects of sildenafil and rosuvastatin on nonadrenergic, non-cholinergic and nitric oxide mediated cavernosal smooth musle relaxation in metabolic syndrome rabbits, since alterations in this pathway are recognised in diabetic and hypercholesterolemic erectile dysfunction. Methods: Ten male rabbits were fed a standart diet as control group, fourty male rabbits were fed a hypercholesterolemic diet for 12 weeks. Hypercholesterolemic group were divided for without treatment, rosuvastatin treatment, sildenafil teratment, and rosuvastatin + sildenafil treatment (N = 10 per groups. Results: Serum levels of cholesterol and glucose were significantly higher in the experimental group than in the control group (p < 0.05. After theraphy no differences were found among the groups in relaxation responses to sodium nitroprusside. The relaxation responses to carbachol and EFS were significantly reduced in metabolic syndrome group to control group (p < 0.05, but there were no differences between the other groups and control group. There was a significantly lower in-vitro relaxation response in the metabolic syndrome rabbits than in controls and the others (p < 0.05. Conclusion: Both agents improve in-vitro relaxation responses of erectile tissue from metabolic syndrome rabbits to endothelial non-adrenergic, non-cholinergic and nitric oxide. This finding supports to the results of other clinical studies with these drugs.

  16. Experimental studies on gas and dust emissions to the atmosphere in rabbit and broiler buildings.

    OpenAIRE

    Calvet Sanz, Salvador

    2010-01-01

    La contaminación atmosférica originada por la producción animal intensiva afecta al medio ambiente global, a la salud de las personas y al bienestar de los animales de la propia granja. Se trata de una problemática de creciente interés en países en los que, como en el caso de España, se ha investigado poco hasta el momento. Esta tesis doctoral se centra en la medición experimental de concentraciones y emisiones de gases (amoniaco, metano, óxido nitroso y dióxido de carbono) y partículas (PM10...

  17. The Relationship between Oxygen Saturation and Color Alteration of a Compromised Skin Flap: Experimental Study on the Rabbit

    Directory of Open Access Journals (Sweden)

    Theddeus O. H. Prasetyono

    2013-09-01

    Full Text Available Background The aim of this study was to collect important data on the time of oxygensaturation change in relation to skin flap color alteration using non-invasive pulse oximetryto evaluate its ability to provide continuous monitoring of skin flap perfusion.Methods An experimental study on the monitoring of blood perfusion of 20 tube-islandgroin flaps of 10 male New Zealand rabbits was performed using pulse oximetry. The animalswere randomly assigned to one of two groups representing a blockage of either arterial orvenous blood flow. The oxygen saturation change and clinical color alteration were monitoredfrom the beginning of vessel clamping until the saturation became undetectable. The resultwas analyzed by the t-test using SSPS ver. 10.0.Results The mean times from the vessel clamping until the saturation became undetectablewere 20.19±2.13 seconds and 74.91±10.57 seconds for the artery and vein clamping groups,respectively. The mean time of the clinical alteration from the beginning of vein clampingwas 34.5±11.72 minutes, while the alteration in flaps with artery clamping could not bedetected until 2.5 hours after clamping.Conclusions The use of neonate-type reusable flex sensor-pulse oximetry is objective andeffective in early detection of arterial and vein blockage. It provides real-time data on vesselocclusion, which in turn will allow for early salvaging. The detection periods of both arterialocclusion and venous congestion are much earlier than the color alteration one may encounterclinically.

  18. Low-level laser therapy: An experimental design for wound management: A case-controlled study in rabbit model

    Directory of Open Access Journals (Sweden)

    Hossein Hodjati

    2014-01-01

    Full Text Available Background: There is a wide array of articles in medical literature for and against the laser effect on wound healing but without discrete effect determination or conclusion. This experimental study aims to evaluate the efficacy of low-level laser therapy on wound healing. Materials and Methods: Thirty-four rabbits were randomly enrolled in two groups after creating a full thickness of 3 × 3 cm wound. The intervention group received low density laser exposure (4 J/cm 2 on days 0, 3 and 6 with diode helium-neon low-intensity laser device (wl = 808 nm and in control group moist wound dressing applied. Finally, wound-healing process was evaluated by both gross and pathological assessment. Results: Fibrin formation was the same in the two groups (P = 0.4 but epithelialisation was much more in laser group (P = 0.02. Wound inflammation of the laser group was smaller than that of the control groups but statistical significance was not shown (P = 0.09. Although more smooth muscle actin was found in the wounds of the laser group but it was not statistically significant (P = 0.3. Wound diameter showed significant decrease in wound area in laser group (P = 0.003. Conclusion: According to our study, it seems that low-level laser therapy accelerates wound healing at least in some phases of healing process. So, we can conclude that our study also shows some hopes for low level laser therapy effect on wound healing at least in animal model.

  19. Magnetic resonance evaluation of luminal patency after polylactide stent implantation: an experimental study in a rabbit aorta model

    International Nuclear Information System (INIS)

    Intravascular metallic stents cause magnetic field distortions, disturbing luminal detection. Our aim was to evaluate the effect of polylactic acid (PLA) stents on magnetic resonance angiography (MRA). Biodegradable double spiral helical PLA stents (n=12) or stainless steel (SS) (n=6) stents were implanted into the infrarenal aortas of New Zealand White rabbits. All SS- and 6 PLA-stented animals as well as 6 non-operated control rabbits were imaged with gadolinium-enhanced MRA (1.5 T), and infrarenal aortic diameters (proximal, distal, and narrowest), together with the stent artifact, were measured. Six of the PLA-stented rabbits were followed up, and MRA was assessed at 2, 6, 9, and 12 months after the stent implantation. Image artifacts caused by the SS stents were visualized in all cases. The PLA stents caused no magnetic field distortion, allowing imaging of the underlying vessel including the vessel lumen. In the follow-up group of 6 rabbits with a PLA stent, 5 of 6 were patent at the end of the follow-up of 12 months. These stents allowed luminal detection at all time points, with no significant differences in aortic measurements emerging during the whole follow-up period. When immediately postoperatively scanned SS and PLA rabbits were compared with controls, no differences were observable in proximal diameters. Instead, the percentage of distal luminal loss was greater in the PLA-stented rabbits, as compared with SS stents (p<0.01). The PLA stenting in small vessels allows evaluation of luminal patency with MRA both immediately after implantation and during follow-up. (orig.)

  20. Nonalcoholic steatohepatitis as a novel player in metabolic syndrome-induced erectile dysfunction: an experimental study in the rabbit.

    OpenAIRE

    Vignozzi L; Filippi S; Comeglio P; Cellai I; Sarchielli E; Morelli A; Rastrelli G; Maneschi E; Galli A; Vannelli GB; Saad F; Mannucci E; Adorini L; Maggi M.

    2014-01-01

    A pathogenic link between erectile dysfunction (ED) and metabolic syndrome (MetS) is now well established. Nonalcoholic steatohepatitis (NASH), the hepatic hallmark of MetS, is regarded as an active player in the pathogenesis of MetS-associated cardiovascular disease (CVD). This study was aimed at valuating the relationship between MetS-induced NASH and penile dysfunction. We used a non-genomic, high fat diet (HFD)-induced, rabbit model of MetS, and treated HFD rabbits with testosterone (T...

  1. Incorporación de aloinjertos óseos intercalares corticales: Estudio experimental en conejos Incorporation of cortical intercalary bone allografts: Experimental study on rabbits

    Directory of Open Access Journals (Sweden)

    S. Amillo

    2003-12-01

    Full Text Available Se realiza un estudio experimental en 40 conejos para valorar la incorporación de grandes aloinjertos óseos intercalares y la influencia de la congelación en el injerto óseo. En 36 conejos adultos se procedió a resecar 3 centímetros diafisarios de tibia y el defecto se reconstruyó con diferente tipo de injerto óseo: autoinjerto fresco y autoinjerto congelado, aloinjerto fresco y aloinjerto congelado; la osteosíntesis se realizó con dos agujas de Kirschner intramedulares. Se hicieron estudios radiológicos mensuales hasta el sacrificio, a los 2, 4 y 8 meses. Se hizo un estudio densitométrico, histológico y de diafanización con técnica de Spalteholz de las tibias. En 24 animales se administraron 25 mg/kg de oxytetraciclina al 3º y 6º días antes del sacrificio para estudiar la velocidad de aposición ósea en el injerto. Observamos una consolidación radiológica buena y excelente en el 80% de los injertos (99% en los autoinjertos y 66% en los aloinjertos. La densidad ósea mostraba un descenso hasta el 4º mes debido a la reabsorción ósea excepto en el grupo de los aloinjertos frescos. La revascularización de los aloinjertos fue más lenta y pobre que en los autoinjertos, pero con el mismo patrón vascular. La incorporación y neoformación ósea fueron mayores en los autoinjertos sin diferencias significativas con los aloinjertos congelados. La velocidad de aposición ósea no se modificó con los diferentes tipos de injertos óseos utilizados. La congelación facilita y acelera la incorporación y cantidad de neoformación ósea de los aloinjertos.An experimental study was carried out on 40 rabbits in order to evaluate the incorporation of large intercalary bone allografts and the influence of freezing on the bone graft. In 36 rabbits resection of 3 centimetres of diaphysis of the tibia was carried out and the defect was rebuilt with different types of bone graft: fresh autograft and frozen autograft, fresh allograft and

  2. In Vivo Efficacy of Moxifloxacin Compared with Cloxacillin and Vancomycin in a Staphylococcus aureus Rabbit Arthritis Experimental Model▿

    OpenAIRE

    Grossi, Olivier; Caillon, Jocelyne; Arvieux, Cedric; Jacqueline, Cedric; Bugnon, Denis; Potel, Gilles; Hamel, Antoine

    2007-01-01

    We investigated the efficacies of moxifloxacin, cloxacillin, and vancomycin in a rabbit model of Staphylococcus aureus arthritis. No significant difference between therapeutic regimens was observed after a 7-day treatment. Oral moxifloxacin could be a suitable alternative to standard parenteral therapy for S. aureus arthritis.

  3. The experimental model applied to the objective estimation of the degree of the rimary reaction development in rabbits under irradiation

    International Nuclear Information System (INIS)

    The facts, characterizing the dependence of the value of clinical development of the primary radiation reaction on manifestation of distortions of hemato-ophthalmic farrier permeability in rabbits are given. It makes possible to apply the method of estimating permeability of ciliary tract vessels in these animals for objective determination of the degree of severity of primary reaction towards irradiation

  4. Anchorage of TiO2-blasted, HA-coated, and machined implants: an experimental study with rabbits

    DEFF Research Database (Denmark)

    Gotfredsen, K; Wennerberg, A; Johansson, C;

    1995-01-01

    rabbit had a machined, a TiO2-blasted, and a TiO2-blasted, HA-coated implant placed in each tibia. After a healing period of 3 and 12 weeks, respectively, the implants placed in the right tibia were used for removal torque test, and the implants placed in the left tibia were used for histomorphometrical...

  5. Anti-inflammatory and antioxidant effects of Tualang honey in alkali injury on the eyes of rabbits: Experimental animal study

    Directory of Open Access Journals (Sweden)

    Sirajudeen KNS

    2011-10-01

    Full Text Available Abstract Background Alkali injury is one of the most devastating injuries to the eye. It results in permanent unilateral or bilateral visual impairment. Chemical eye injury is accompanied by an increase in the oxidative stress. Anti-inflammatory and antioxidant agents play a major role in the treatment of chemical eye injuries. The purpose of this study is to evaluate the anti-inflammatory (clinical and histopathological and antioxidant effects of Tualang honey versus conventional treatment in alkali injury on the eyes of rabbits. Methods A preliminary study was carried out prior to the actual study to establish the alkali chemical injury on rabbit's cornea and we found that alkali chemical injury with 2 N NaOH showed severe clinical inflammatory features. In actual study, alkali injury with 2 N NaOH was induced in the right eye of 10 New Zealand White rabbits' cornea. The rabbits were divided into two groups, Group A was given conventional treatment and Group B was treated with both topical and oral Tualang honey. Clinical inflammatory features of the right eye were recorded at 12 hours, 24 hours, 72 hours, 5th day and 7th day post induction of alkali burn on the cornea. The histopathological inflammatory features of the right corneas of all rabbits were also evaluated on day-7. The level of total antioxidant status and lipid peroxidation products in the aqueous humour, vitreous humour and serum at day-7 were estimated biochemically. Fisher's Exact, Chi-Square and Mann-Whitney test were used to analyse the data. Results There was no statistically significant difference in clinical inflammatory features (p > 0.05 between honey treated and the conventional treated group at different times of examination. Histopathological examination of the cornea showed the number of polymorphonuclear leucocytes was below 50 for both groups (mild grade. There was also no significant difference in the level of total antioxidant status as well as lipid

  6. Enhanced Ablation of High Intensity Focused Ultrasound with Microbubbles: An Experimental Study on Rabbit Hepatic VX2 Tumors

    International Nuclear Information System (INIS)

    Purpose: This study was designed to assess the enhanced effect of high intensity focused ultrasound (HIFU) ablation with microbubbles on rabbit hepatic VX2 tumors and to compare the detection sensitivity of CEUS and CECT to determine the residual viable tissue after ablation of HIFU. Methods: Forty rabbits with hepatic VX2 tumors were randomly separated into two groups (20 animals per group) before HIFU ablation. A bolus of 0.2 mL of saline or a microbubble-based ultrasound (US) contrast agent was injected intravenously to group I rabbits and group II rabbits, respectively. The HIFU ablation procedure was started 15 s after the injection. Tumors were examined with grayscale contrast-enhanced ultrasound (CEUS) and contrast-enhanced computed tomography (CECT) immediately before and after HIFU ablation. Histopathologic assessment was performed immediately after treatment imaging. Results: Before ablation, intense contrast enhancement during arterial phase was observed at the whole tumors or the periphery of the tumors by CEUS and CECT. Lower HIFU energy was used in group II than in group I (P < 0.001). Histopathologic assessment revealed local residual viable tumor tissues due to incomplete ablation in 47.4% (9/19) of tumors in group I and 10% (2/20) of tumors in group II (P < 0.05). The concordance rate of CEUS (90.9%) with histopathology on residual tumor detection was higher than that of CECT (27.3%, P < 0.05). Conclusions: Introduction of the microbubble agent enhances HIFU therapeutic efficacy. CEUS proves to have high sensitivity in assessment of residual viable rabbit VX2 tumor after HIFU.

  7. The establishment of experimental chronic osteomyelitis induced by staphylococcus aureus in rabbits%兔胫骨慢性骨髓炎动物模型的建立

    Institute of Scientific and Technical Information of China (English)

    卢旻鹏; 蒋电明; 权正学; 黄伟; 曹何; 况尚如; 李广州

    2010-01-01

    Objective To analyze the relationship between the inoculation dose of the bacteria of Staphylococcus aureus ATCC25923 and the severity of experimental Staphylococcus aureus osteomyelitis in rabbits. Methods Thirty-six healthy New Zealand white rabbits were randomized into 4 groups: control group and experimental groups A, B, C. There were 10 rabbits in every experimental group and 6 rabbits in control group. Five percent sodium morrhuate (0. 1 ml) and serial dilutions of the bacteria of Staphylococcus aureus ATCC25923 [3 × 108 to 3 × 109 colony-forming units ( CFU)/ml]suspended in saline or saline alone were inoculated into the proximal metaphysis of the tibia. No antibiotics were used to prevent fatal sepsis. The severity of experimental Staphylococcus aureus osteomyelitis in rabbits was evaluated by clinical, radiologic,bacteriologic and histologic parameters at the 4th week after infection. Results There was clinical progression of the disease observed by draining wounds, a postoperative limp that subsided in all rabbits, and varied periods of anorexia despite an average increase in body weight. There were varying degrees of BMD decrease, decreased bone trabeculae and bone trabecular space widening in experimental groups at the 4th week after infection.Slices indicated that there were varying degrees of neutrophil infiltration, necrosis of marrow cells and interstitial hemorrhage. Gross pathologic and radiographic criteria were used to grade the severity of experimental staphylococcus aureus osteomyelitis in rabbits. The grading of experimental groups A, B, and C was respectively stage 1 to 2 osteomyelitis, stage 2 to 3 osteomyelitis, stage 3 to 4 osteomyelitis. Conclusion The bacteria of Staphylococcus aureus ATCC25923 suspended in saline was inoculated into the proximal metaphysis of the tibia and can induce osteomyelitis. The severity of experimental Staphylococcus aureus osteomyelitis in rabbits was aggravated with the increase of the bacteria amount

  8. Detection of experimental thrombi in rabbits with an 131I-labelled fibrin-specific monoclonal antibody

    International Nuclear Information System (INIS)

    The detection of thrombi in rabbits has been investigated with 131I-labelled DD-3B6/22, a monoclonal antibody (Mab) reactive at high affinity (Kd=2.68x10-10 M) with human D Dimer (DD). DD-3B6/22 bound well to both 'fresh' and 'aged' human clots in an in vitro assay but showed poor binding to rabbit clots. However, reactivity was restored to rabbit blood if it was seeded, before clotting, with human DD covalently coupled to Sepharose beads. Thus, a rabbit model was developed in which blood was allowed to clot around DD-Sepharose beads introduced into the jugular vein. Gamma camera imaging showed that intact 131I-labelled DD-3B6/22 localised to these clots within 24 h. Uptake at this time was 0.202%±0.012% injected dose per gram (%ID/g) compared with 0.086±0.018%ID/g after injection of control antibody. 131I-labelled F(ab')2 fragments of DD-3B6/22 allowed earlier scintigraphic detection of the clot which was evident 4 h after injection. Uptake in the clot at 24 h was 0.154±0.038% ID/g compared with 0.109±0.027% ID/g for a control F(ab')2. As antigen levels in the clot are estimated to be less than 300 μg DD, thus representing a very small human clot, the DD-3B6/22 Mab would appear to have a good potential for the sensitive detection of thrombi in a clinical setting. (orig.)

  9. STUDIES IN EXPERIMENTAL SYPHILIS : VII. REINOCULATION OF TREATED AND UNTREATED SYPHILITIC RABBITS WITH HETEROLOGOUS STRAINS OF TREPONEMA PALLIDUM.

    Science.gov (United States)

    Chesney, A M; Halley, C R; Kemp, J E

    1927-07-31

    Syphilitic rabbits, whether untreated or treated after the 90th day of infection, were found to be more refractory to subsequent inoculation with the homologous strain of Treponema pallidum than to inoculation with heterologous strains of the same organism, when clinical criteria alone were employed in judging the outcome of reinoculation. The incidence of second infection with homologous strains was 5.4 per cent, as against 50 per cent with heterologous strains.(2) The resistance which develops in rabbits during the course of a syphilitic infection appears therefore to be strain-specific rather than species-specific. The protection afforded against homologous strains was found to persist for at least as long as 6 months after treatment was discontinued. A given strain may afford a higher degree of protection against some strains than against others, but whether this is to be explained upon the basis of biologic relationship or of differences in virulence, or possibly as the result of both of these factors was not disclosed by the experiments. Rabbits infected with a strain (Nichols) which had been adapted to this species for over a decade could be infected with strains which had been recovered recently from the human body. The previous existence of a syphilitic lesion in the testis which was used as the site for reinoculation did not seem to exert any influence upon the incidence of successful second infections obtained with heterologous strains of Treponema pallidum. Sometimes the course of the second infection produced by inoculation with heterologous strains was less pronounced than that observed in the controls, but in most instances no significant alteration was observed. In syphilitic rabbits treated late in the course of the disease and reinoculated with heterologous strains of Treponema pallidum no lesion may develop at the site of reinoculation but nevertheless the Wassermann reaction may become positive and remain so for weeks thereafter. It is suggested

  10. Experimental poisoning by Callaeum psilophyllum in rabbits and goatsIntoxicação experimental por Callaeum psilophyllum em coelhos e caprinos

    Directory of Open Access Journals (Sweden)

    Amélia Lizziane Leite Duarte

    2013-10-01

    Full Text Available Callaeum (Mascagnia psilophyllum is found in the same regions than Amorimia (Mascagnia rigida, a sodium monofluoroacetate containing plant that cause sudden death associated with exercise in ruminants. With the objective to determine the toxicity of C. psilophyllum, leaves of the plant were administered to rabbits and goats. In rabbits doses of 6-12 g.kg-1 body weight caused tachycardia, dyspnea, and mydriasis. One rabbit, that ingested 10 g/kg, showed clinical signs similar to those caused by A. rigida in rabbits. Four goats showed diarrhea after the ingestion of green or dry leaves of the plant. Three died and one recovered. The results in rabbits suggest that C. psilophyllum contains fluoroacetate. However, clinical signs in goats suggest that C. psilophyllum contains another substance responsible for the digestive signs. Callaeum (Mascagnia psilophyllum é encontrada nas mesmas regiões que Amorimia (Mascagnia rigida, uma planta que contêm monofluoroacetato de sódio e causa morte súbita associada ao exercício em ruminantes. Com o objetivo de determinar a toxicidade de C. psilophyllum, folhas desta planta foram administradas a coelhos e caprinos. Em coelhos, doses de 6-12 g.kg-1 de peso corporal causaram taquicardia, dispneia e midríase. Um coelho, que ingeriu 10 g.kg-1, apresentou sinais clínicos semelhantes aos causados por A. rigida em coelhos. Em caprinos, quatro animais apresentaram diarreia após a ingestão de folhas verdes ou secas da planta. Três caprinos morreram e um se recuperou. Os resultados em coelhos sugerem que C. psilophyllum contêm fluoroacetato. No entanto, os sinais clínicos em caprinos sugerem que C. psilophyllum contêm outra substância responsável pelos sinais digestivos.

  11. Iodine-125 Seeds Strand for Treatment of Tumor Thrombus in Inferior Vena Cava: An Experimental Study in a Rabbit Model

    International Nuclear Information System (INIS)

    Objective: The purpose of this study was to establish an animal model of implanted inferior vena cava tumor thrombus (IVCTT) and to evaluate the effect of linear iodine-125 seeds strand in treating implanted IVCTT. Methods: Tumor cell line VX2 was inoculated subcutaneously into New Zealand rabbit to develop the parent tumor. The tumor strip was inoculated into inferior vena cava (IVC) to establish the IVCTT model. The IVCTT was confirmed by multidetector computed tomography (MDCT) after 2 weeks. Twelve rabbits with IVCTT were randomly divided into two groups. Treatment group (group T; n = 6) underwent Iodine-125 seeds brachytherapy, and the control group (group C; n = 6) underwent blank seeds strand. The blood laboratory examination (including blood routine examination, hepatic and renal function), body weight, survival time, and IVCTT volume by MDCT were monitored. All rabbits were dissected postmortem, and the therapeutic effects were evaluated on the basis of histopathology. The proliferating cell nuclear antigen index (PI) and apoptosis index (AI) of IVCTT were compared between two groups. T test, Wilcoxon rank test, and Kaplan–Meier survival curve analysis were used. Results: The success rate of establishing IVCTT was 100 %. The body weight loss and cachexia of rabbits in group C appeared earlier than in group T. Body weight in the third week, the mean survival time, PI, AI in groups T and C were 2.23 ± 0.12 kg, 57.83 ± 8.68 days, (16.73 ± 5.18 %), (29.47 ± 7.18 %), and 2.03 ± 0.13 kg, 43.67 ± 5.28 days, (63.01 ± 2.01 %), (6.02 ± 2.93 %), respectively. There were statistically significant differences between group T and group C (P < 0.05). The IVCTT volume of group T was remarkably smaller than that of group C. Conclusions: Injecting and suspensory fixing VX2 tumor strip into IVC is a reliable method to establish IVCTT animal model. The linear Iodine-125 seeds strand brachytherapy was a safe and effective method for treating IVCTT in rabbit model

  12. Ultrasonography and Radiography Evaluation of the Cartilage Graft in Repair of Experimentally Induced Radial Bone Defect in Rabbit

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    Foad Sadi

    2010-01-01

    Full Text Available We would like to thank to the Faculty of Specialized Veterinary Sciences research council. Science and Research Branch of Islamic Azad University, Punak Tehran for approval and financial support to finish this project. Problems statement: The purpose of this research was to determine the biological effect of cartilage graft as a bone defect filler and osteogenetic stimulation to speed up bone healing too. Approach: Sixteen adult male New Zealand white rabbits having body weight ranged from 3.0-3.5 Kg. Under general anesthesia, a segmental full thickness bone defect of 10 mm in length was created in the middle of the right radial shaft in all rabbits. They were divided into two groups of 6 rabbits each. Group I was considered as control and the fractured site was fixed using finger bone plate with 4 screws, whereas the ear cartilage of 1×1 cm graft was used to fill the gap after fracture fixation in Group II. Rabbits in two groups were subdivided into 2 subgroups of 1 and 2 months duration with 4 rabbits in each. Radiography and two dimensional and color Doppler sonography were done before and after creating defects and on 15, 30 and 60 days to evaluate local reaction as far as new blood vessels network and callus formation are concerned. Results: On the radiographs during the whole process, bone repair in Group I was not as perfect as those in Group II samples and trace of internal callus filled the gap incompletely in 60 days in Group I, whereas in Group II internal callus almost was formed on 30 days and in addition intercortical callus was seen supporting to cover and filled the gap completely in this group. Sonographic findings confirmed the protrusion of newly formed blood vascular network in 30 days in Group I and from 15 days in Group II and remarkably increased till end of observation period. Conclusion: Cartilage graft is suitable alternative bone filler and radiography and sonography are reliable techniques to trace local reaction at

  13. Iodine-125 Seeds Strand for Treatment of Tumor Thrombus in Inferior Vena Cava: An Experimental Study in a Rabbit Model

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Wen, E-mail: wenzhangxiao@126.com; Yan, Zhiping, E-mail: Yan.zhiping@zs-hospital.sh.cn; Luo, Jianjun, E-mail: luo.jianjun@zs-hospital.sh.cn; Fang, Zhuting, E-mail: 470389481@qq.com; Wu, Linlin, E-mail: linlinzhifubao@126.com; Liu, QingXin, E-mail: liu.qingxin@zs-hospital.sh.cn; Qu, Xudong, E-mail: qu.xudong@zs-hospital.sh.cn; Liu, Lingxiao, E-mail: liu.lingxiao@zs-hospital.sh.cn; Wang, Jianhua, E-mail: wang.jianhua@zs-hospital.sh.cn [Fudan University, Department of Interventional Radiology, Zhongshan Hospital (China)

    2013-10-15

    Objective: The purpose of this study was to establish an animal model of implanted inferior vena cava tumor thrombus (IVCTT) and to evaluate the effect of linear iodine-125 seeds strand in treating implanted IVCTT. Methods: Tumor cell line VX{sub 2} was inoculated subcutaneously into New Zealand rabbit to develop the parent tumor. The tumor strip was inoculated into inferior vena cava (IVC) to establish the IVCTT model. The IVCTT was confirmed by multidetector computed tomography (MDCT) after 2 weeks. Twelve rabbits with IVCTT were randomly divided into two groups. Treatment group (group T; n = 6) underwent Iodine-125 seeds brachytherapy, and the control group (group C; n = 6) underwent blank seeds strand. The blood laboratory examination (including blood routine examination, hepatic and renal function), body weight, survival time, and IVCTT volume by MDCT were monitored. All rabbits were dissected postmortem, and the therapeutic effects were evaluated on the basis of histopathology. The proliferating cell nuclear antigen index (PI) and apoptosis index (AI) of IVCTT were compared between two groups. T test, Wilcoxon rank test, and Kaplan-Meier survival curve analysis were used. Results: The success rate of establishing IVCTT was 100 %. The body weight loss and cachexia of rabbits in group C appeared earlier than in group T. Body weight in the third week, the mean survival time, PI, AI in groups T and C were 2.23 {+-} 0.12 kg, 57.83 {+-} 8.68 days, (16.73 {+-} 5.18 %), (29.47 {+-} 7.18 %), and 2.03 {+-} 0.13 kg, 43.67 {+-} 5.28 days, (63.01 {+-} 2.01 %), (6.02 {+-} 2.93 %), respectively. There were statistically significant differences between group T and group C (P < 0.05). The IVCTT volume of group T was remarkably smaller than that of group C. Conclusions: Injecting and suspensory fixing VX2 tumor strip into IVC is a reliable method to establish IVCTT animal model. The linear Iodine-125 seeds strand brachytherapy was a safe and effective method for treating IVCTT

  14. Comparison of 1% cyclosporine eye drops in olive oil and in linseed oil to treat experimentally-induced keratoconjunctivitis sicca in rabbits

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    Leticia Rodrigues Parrilha

    2015-10-01

    Full Text Available ABSTRACTPurpose:To evaluate the effectiveness of topical 1% cyclosporine eye drops diluted in either of the two vehicles-olive and linseed oil-and that of the oils themselves in treating experimentally-induced keratoconjunctivitis sicca (KCS in rabbits.Methods:KCS was induced in 25 New Zealand rabbits using 1% atropine sulfate eye drops for 7 days before treatment and throughout the treatment period (12 weeks. The rabbits were divided into five groups: one control (C group without KCS induction and four treatment groups in which KCS was induced and treated topically with olive oil (O, linseed oil (L, cyclosporine in olive oil (CO, and cyclosporine in linseed oil (CL. The animals were evaluated using Schirmer tear test 1 (STT, the fluorescein test (FT, tear-film break-up time (TBUT, the rose bengal test (RBT, and histopathological analysis.Results:Values of STT and TBUT significantly decreased 1 week post-induction (p<0.05 and were similar to initial values after the 4th week of treatment, in all groups. After KCS induction, there was significantly less corneal damage in group L than in group CL, as assessed FT and RBT. Histopathology demonstrated that Groups L and CL presented less edema and corneal congestion. There was no significant difference in the goblet cell density (cells/mm2 between the groups (p=0.147.Conclusion:Cyclosporine diluted in olive oil or linseed oil was effective in the treatment of KCS, although it had better efficacy when diluted in linseed oil. Linseed oil presented better effectiveness, whether associated or not, than olive oil. These results may contribute to the creation of novel topical ophthalmic formulations for KCS treatment in future.

  15. The embolization effect of tanshinone II A-polylactic acid/glycolic acid microspheres on the hepatic artery in experimental rabbits

    International Nuclear Information System (INIS)

    Objective: To observe the embolization effect of tanshinone II A-polylactic acid/glycolic acid microspheres (PLGA) on the hepatic artery in experimental rabbits. Methods: Under DSA guidance, hepatic catheterization and angiography was performed in 24 New Zealand white rabbits. Via the catheter tanshinone II A-PLGA microspheres was injected into the hepatic arteries. Each time at 10 minutes and on the 1 st, 3 rd, 7 th, 14 th, 21 st, 30 th and 42 nd day after the treatment, every three rabbits were randomly selected for the re-examination with angiography to observe the embolization state of the hepatic arteries, then the animals were sacrificed and the liver, heart, spleen, lung kidney and stomach were removed and sent for pathologic exam. Simultaneously, the functional tests of liver and kidney as well as the routine blood tests were made. The results were evaluated. Results: The peripheral micro-vessels of the hepatic artery vanished away at 10 minutes after embolization, and they remained un-visualized till the 30th day after embolization. On the 42nd day after embolization the peripheral micro-vessels of the hepatic artery could be visualized again. Pathologically,signs of inflammation and necrosis appeared in the occluded areas. Blood biochemical examination showed that there was a transient elevation of white blood cells after the procedure, which fell to normal level on the 7th day (P > 0.05). Both AST and ALT reached their peaks on the 3rd day, then they decreased gradually and returned to normal on the 7th day (P > 0.05). Conclusion: Tanshinone II A-PLGA microspheres have better embolization effect on the hepatic arteries, this effect lasts for 30 to 42 days. Therefore, tanshinone II A-PLGA microsphere is an ideal embolization agent for the treatment of neoplasm. (authors)

  16. Recombinant Rabbit Leukemia Inhibitory Factor and Rabbit Embryonic Fibroblasts Support the Derivation and Maintenance of Rabbit Embryonic Stem Cells

    OpenAIRE

    Xue, Fei; Ma, Yinghong; Chen, Y. Eugene; Zhang, Jifeng; Lin, Tzu-An; Chen, Chien-Hong; Lin, Wei-Wen; Roach, Marsha; Ju, Jyh-Cherng; Yang, Lan; Du, Fuliang; Xu, Jie

    2012-01-01

    The rabbit is a classical experimental animal species. A major limitation in using rabbits for biomedical research is the lack of germ-line-competent rabbit embryonic stem cells (rbESCs). We hypothesized that the use of homologous feeder cells and recombinant rabbit leukemia inhibitory factor (rbLIF) might improve the chance in deriving germ-line-competent rbES cells. In the present study, we established rabbit embryonic fibroblast (REF) feeder layers and synthesized recombinant rbLIF. We der...

  17. Transarterial embolization with low concentration of n-butyl cyanoacrylate in VX2 hepatic tumor rabbit: an experimental study

    International Nuclear Information System (INIS)

    Objective: To investigate the feasibility, safety and efficacy of transarterial embolization with low concentration of n-butyl cyanoacrylate (NBCA) in rabbit VX2 liver tumor models. Methods: Twenty-four rabbits were implanted with VX2 hepatic tumors into the left hepatic lobes, and were scanned with CT to measure the volume of the tumor after 14 days. They were randomly divided into three groups with 8 rabbits assigned to each group. Transarterial embolization was conducted with physiological saline in control group A, with pure Lipiodol in group B, with 2.5% NBCA in group C. Hepatic toxicity was evaluated by blood biochemical analysis of the plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST). One week later, the volumes of the tumors were measured by CT again. Tumor growth rate was the ratio of tumor's volume at 7th day after embolization to the tumors' volume before embolization. The survival periods of the rabbits of the three groups after treatment were also recorded. The data of ALT and AST mean values from each group were analyzed with repeated measurement analysis of variance (ANOVA). Tumor growth rates and survival periods were analyzed by using one-way ANOVA. Results: All animal models were successfully established and underwent interventional catheterization. Both ALT and AST mean values of the rabbits in group A, B and C at each time point before and after embolization were significantly different (ALT F=10.508, 16.443, 19.828, respectively; AST F= 23.696, 23.334, 15.594, respectively) (P<0.05). ALT in group A, B, C were (49.4±13.5), (115.2±48.8), (124.7±49.4) U/L, while AST in group A, B, C were (52.3±12.0), (128.3± 50.1), (137.0±66.9) U/L 4 days after embolization. The ALT and AST mean values were significantly elevated 4 days after embolization in group B and group C compared with those before embolization and those of group A 4 days after treatment (P<0.05). However, the ALT and AST mean values showed no statistically

  18. Analysis of efficiency of the double- centrifugation protocol to prepare platelet rich plasma (PRP – an experimental study in rabbits

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    Michel Reis MESSORA

    2009-09-01

    Full Text Available Introduction and objective:The purpose of this study was to evaluatethe concentrations of platelets obtained from platelet rich plasma (PRP prepared according to the double-centrifugation protocol. Material and methods: Eight adult male rabbits (White New Zealand weighing 2.8 to 4 kg were used.After general anesthesia, 10 ml of blood were drawn from each animal via cardiac puncture. Each blood sample was centrifuged according to the protocol of Sonnleitner et al.(2000.The peripheral blood (total from each animal and the PRP samples platelets were counted manually. Data were submitted to statistical analysis.The normality of the data was confirmed and the Student’s t test was applied (p < 0.05.Results: PRP samples presented an average platele count significantly higher than that of peripheral blood.Conclusion Within the limits of this study, it was concluded that the double centrifugation protocol was adequate to prepare PRP .

  19. Bone regeneration and infiltration of an anisotropic composite scaffold: an experimental study of rabbit cranial defect repair.

    Science.gov (United States)

    Li, Jidong; You, Fu; Li, Yubao; Zuo, Yi; Li, Limei; Jiang, Jiaxing; Qu, Yili; Lu, Minpeng; Man, Yi; Zou, Qin

    2016-01-01

    Tissue formation on scaffold outer edges after implantation may restrict cell infiltration and mass transfer to/from the scaffold center due to insufficient interconnectivity, leading to incidence of a necrotic core. Herein, a nano-hydroxyapatite/polyamide66 (n-HA/PA66) anisotropic scaffold with axially aligned channels was prepared with the aim to enhance pore interconnectivity. Bone tissue regeneration and infiltration inside of scaffold were assessed by rabbit cranial defect repair experiments. The amount of newly formed bone inside of anisotropic scaffold was much higher than isotropic scaffold, e.g., after 12 weeks, the new bone volume in the inner pores was greater in the anisotropic scaffolds (>50%) than the isotropic scaffolds (<30%). The results suggested that anisotropic scaffolds could accelerate the inducement of bone ingrowth into the inner pores in the non-load-bearing bone defects compared to isotropic scaffolds. Thus, anisotropic scaffolds hold promise for the application in bone tissue engineering. PMID:26775692

  20. Kinetic analysis of experimental rabbit tumour and inflammation model with 18F-FDG PET/CT

    International Nuclear Information System (INIS)

    Non-specific accumulation of 18F-FDG by both tumour and inflammatory lesions can make diagnostic analysis difficult. Our aim was to explore the difference in 18F-FDG uptake kinetics between tumour and inflammatory cells. To this end, we investigated VX2 tumour lesions and inflammatory lesions in rabbits. Methods: Six rabbits with VX2 tumour cells transplanted into one forelimb muscle and inflammatory lesions induced by turpentine oil in the contralateral forelimb were scanned for 60 minutes post 18F-FDG injection. Imaging data was analyzed with the standard 2-tissue-compartment model. Parameters, VB, Ki, K1, k2, k3, k4, were compared between tumour and inflammatory lesions. SUV and dual time scan methods were also compared in the experiment. Results: Time activity curves of VX2 tumour lesions showed a characteristic pattern of gradually increasing 18F-FDG uptake up to 60 min, whereas, 18F-FDG uptake in inflammatory lesions increased more slowly than in tumours. Parameters estimated from the uptake process showed that forward transport constant, K1, and influx constant, Ki, values in VX2 tumour lesions (0.186 ± 0.053 and 0.048 ± 0.014, respectively) was significantly higher than that in inflammatory lesions (0.129 ± 0.024 and 0.022 ± 0.007, respectively) (p 18F-FDG injection were also significantly higher in the VX2 tumor lesions than in the inflammatory lesions. Retention index (RI) was not significantly different between VX2 tumours and inflammatory lesions (1.134 ± 0.076 vs. 1.060 ± 0.058, p > 0.05). Conclusion: Different kinetic parameters (Ki, K1, k3) exist between inflammatory and tumour lesions. (orig.)

  1. Tumor hepático experimental (VX-2 em coelho: implantação do modelo no Brasil Experimental liver tumor (VX-2 in rabbits: implantation of the model in Brazil

    Directory of Open Access Journals (Sweden)

    Rogério Saad Hossne

    2002-08-01

    Full Text Available Os estudos para a investigação de novas modalidades terapêuticas em biologia tumoral, deveriam passar por estudos experimentais prévios. Neste sentido dispõem-se hoje de uma grande variedade de modelos tumorais experimentais; em determinadas investigações faz-se necessária a adequação do modelo tumoral às necessidades biológicas, patológicas e experimentais dos estudos. Desta forma, em nosso serviço, buscávamos um modelo tumoral hepático para estudos experimentais que se adequasse às seguintes características: fácil manipulação, crescimento controlável, evolução e agressividade semelhantes aos seres humanos. Os dados da literatura nos levaram a busca do tumor hepático VX-2, em coelhos. Neste artigo discutimos as vantagens da utilização deste modelo experimental e a sua introdução em nosso país.Studies for investigation of new therapeutic modalities in tumoral biology should be based on previous experimental studies. Then, there are a great variety of tumoral experimental models today. Some investigations have been done necessary an adaptation of the tumoral model to the needing of the studies biological and pathological. So, in our laboratory, we looked for a tumoral hepatic model for experimental studies with the following characteristics: easy manipulation, control of growing, evolution and aggressiveness like to humans. Data of the literature took us the search of the hepatic tumor VX-2, in rabbits. In this article we discussed the advantages of use this experimental model and its introduction in our country. Experimental hepatic tumor (VX-2 in rabbit. Implantation of the model in Brazil.

  2. An experimental study on the effect of mixture of absolute ethanol and lipiodol injected into normal liver of rabbit : CT features and histopathologic changes

    International Nuclear Information System (INIS)

    To investigate the safety and usefulness of Lipiodol-percutaneous transhepatic ethanol injection(LPEI) and to determine the appropriate concentration of Lipiodol during L-PEI. This was achieved by evalvating CT findings and histopathologic changes according to the concentration of Lipiodol, amount of ethanol, and the time interval after injection into normal rabbit liver. This experimental study involved 18 New Zealand rabbits under US guidance. They were divided into five groups according to injected materials; two rabbits with 0.4cc of normal saline(group I), six with 0.4cc of ethanol in the left hepatic lobe(group II), and 0.4cc of Lipiodol in the right hepatic lobe(group III), five rabbits with 5% Lipiodol-ethanol(5% vol. of Lipiodol+95% vol. of ethanol), 0.2cc in the right hepatic lobe, and 0.4cc in the left(group IV); and five rabbits with 10% Lipiodol-ethanol as per group IV(group V). CT was performed immediately, one week, two weeks, and three-four weeks after injection, and pathologic specimens were obtained on the third day(acute phase) and during the third or fourth week(chronic phase) after injection. On CT, intrahepatic localization of the L-PEI injection site was well demonstrated as a focal high attenuated area which gradually decreased in attenuation on follow up CT. The opacification of the inferior vena cava by Lipiodol, the linear distribution of Lipiodol along portal veins or fissures, and peritoneal leakage were clearly demonstrated in groups III-V, though the effects gradually disappeared during follow-up CT. There was no remarkable difference in gross CT attenuation between group IV and group V. The main pathologic findings during the acute phase of group II were coagulation necrosis surrounded by macrophage, inflammatory reaction, and early periportal and subcapsular fibrosis. The findings in group IV and V were similar to those in group II and additional fat vacuole accumulations in the necrotic area were also seen. During the chronic phase

  3. Combined intra-arterial thrombolysis and neuprotectant agents reduce cerebral infarction in rabbits with experimental acute cerebral ischemia

    Institute of Scientific and Technical Information of China (English)

    Pei Shi

    2006-01-01

    BACKGROUND:The intra-arterial thrombolytic therapy is one of main methods for more patients to obtain bene-fits.The percentage of arterial recanalization treated with intre-arterial therapy is higher than with intra-venous therapy.next,the dose of thrombolytic medicines is lower and the therapeutic time window may be possibly longer.Related researches are focus on intra-artedal thrombolysis combining with neuprotectant agents to treat acute ischemic stroke.The results show that combination of them can further prolong the therapeutic time window.improve the percentage of arterial recanalization and reduce cerebral infarction volume.OBJECTIVE:To observe the effect of single thmmbolitic therapy combined with neuroprotectant agents in the treatment of acute ischemic stroke.DESIGN:Randomized block design.SETTING:Xinhua Hospital of Xixiang City.Henan Province.MATERIALS:Thirty-six adult male white rabbits.weighing 1.5-2.0 kg.dean grade.were provided by Expedmental Animal Center of Xinxiang Medical College.All rabbits were randomly divided into three groups:intra-arterial thrombolysis control group.corenalin control group and combination group with 12 in each group.Urekinase was provided by Beijing Saisheng Pharmaceutical Co.,Ltd.(batch number:020923);corenalin by Sanjing Pharmaceutical Co.,Ltd.of Harbin Pharmacautical Group(batch number:021106):nimodipine by Shandong Xihua Pharmaceutical Co.,Ltd.(batch number:020611):contrast medium IOPAMlR0300 by Bracco s.P.a.Milano italian (batch number:0584);2,3,5-triphenyltetrazolium chloride(TTC)by Beijing Mashi Fine ChemicaL Product Co.,Ltd.(batch number:020926).METHODS: The experiment was camed out in the Department of Intervention. Second People's Hospital of Xinxiang from September 2002 to May 2003.①According to techniques of Benes et al and Zhu et al,animal models with acute ischemia were established.Two hours later.the therapy began.Intra-artedal thrombolysis control group:5 000 U/kg urokinase was dripped in Ieft common

  4. Combined intra-arterial thrombolysis and neuprotectant agents reduce cerebral infarction in rabbits with experimental acute cerebral ischemia

    Institute of Scientific and Technical Information of China (English)

    Pei Shi

    2006-01-01

    BACKGROUND:The intra-arterial thrombolytic therapy is one of main methods for more patients to obtain bene-fits.The percentage of arterial recanalization treated with intre-arterial therapy is higher than with intra-venous therapy.next,the dose of thrombolytic medicines is lower and the therapeutic time window may be possibly longer.Related researches are focus on intra-artedal thrombolysis combining with neuprotectant agents to treat acute ischemic stroke.The results show that combination of them can further prolong the therapeutic time window.improve the percentage of arterial recanalization and reduce cerebral infarction volume.OBJECTIVE:To observe the effect of single thmmbolitic therapy combined with neuroprotectant agents in the treatment of acute ischemic stroke.DESIGN:Randomized block design.SETTING:Xinhua Hospital of Xixiang City.Henan Province.MATERIALS:Thirty-six adult male white rabbits.weighing 1.5-2.0 kg.dean grade.were provided by Expedmental Animal Center of Xinxiang Medical College.All rabbits were randomly divided into three groups:intra-arterial thrombolysis control group.corenalin control group and combination group with 12 in each group.Urekinase was provided by Beijing Saisheng Pharmaceutical Co.,Ltd.(batch number:020923);corenalin by Sanjing Pharmaceutical Co.,Ltd.of Harbin Pharmacautical Group(batch number:021106):nimodipine by Shandong Xihua Pharmaceutical Co.,Ltd.(batch number:020611):contrast medium IOPAMlR0300 by Bracco s.P.a.Milano italian (batch number:0584);2,3,5-triphenyltetrazolium chloride(TTC)by Beijing Mashi Fine ChemicaL Product Co.,Ltd.(batch number:020926).METHODS: The experiment was camed out in the Department of Intervention. Second People's Hospital of Xinxiang from September 2002 to May 2003.①According to techniques of Benes et al and Zhu et al,animal models with acute ischemia were established.Two hours later.the therapy began.Intra-artedal thrombolysis control group:5 000 U/kg urokinase was dripped in Ieft common

  5. Estudo experimental da influência da atividade muscular da face sobre o esqueleto da mesoestrutura facial em coelhos Experimental study on the influence of facial muscle activity on the facial mesostructure bones in rabbits

    Directory of Open Access Journals (Sweden)

    Andre Ricardo Mateus

    2008-10-01

    Full Text Available A partir do conceito da matriz funcional, surgiu a hipótese de que são os tecidos moles atuando sobre determinada peça óssea que determinam o processo de crescimento facial. A possibilidade de modificar a influência muscular, seja na fase de desenvolvimento facial, seja em pós-operatórios de cirurgia corretiva é de grande importância preventiva e deveria ser mais bem investigada, uma vez que poderia subtrair o número e magnitude destes procedimentos. DESENHO DO ESTUDO: Experimental em coelhos. OBJETIVO: Estimar a relevância da atividade muscular sobre o esqueleto facial, em coelhos de experimentação, durante sua fase de desenvolvimento facial. MATERIAL E MÉTODO: Foram estudados 37 coelhos de 2 meses de idade, divididos em grupo de estudo e grupo controle e seguidos por um período de 4 meses. Os animais do grupo de estudo tiveram seus nervos faciais seccionados no seu ramo cervical unilateralmente. O esqueleto da mesoestrutura facial era retirado para estudo morfométrico por programa de computação gráfica em fotografias digitalizadas realizadas nas peças. Os resultados obtidos sofreram análise estatística comparativa. CONCLUSÃO: Ausência de atividade muscular em uma metade da face produz desvio lateral da mesoestrutura facial para o mesmo lado em coelhos em desenvolvimento.Based on the functional matrix concept, scientists developed the hypothesis that soft tissue acting on certain bone pieces determines the process of facial growth. The possibility to modify muscle influence in the phase of facial development, or in postoperative of corrective surgery is of great preventive importance and it should be better investigated, since it could reduce the number and impact of these procedures. STUDY DESIGN: experimental in rabbits. AIM: to estimate the relevance of facial muscle activity on facial bones in lab rabbits. MATERIALS AND METHODS: 37 rabbits of two months of age were studied, divided in a study group and a control

  6. Kinetic analysis of experimental rabbit tumour and inflammation model with {sup 18}F-FDG PET/CT

    Energy Technology Data Exchange (ETDEWEB)

    Liu, P. [Dept. of Nuclear Medicine, Ninth People' s Hospital, Medical School of Jiaotong Univ., SH (China); Huang, G.; Dong, S.; Wan, L. [Dept. of Nuclear Medicine, Renji Hospital, Medical School of Jiaotong Univ., SH (China)

    2009-07-01

    Non-specific accumulation of {sup 18}F-FDG by both tumour and inflammatory lesions can make diagnostic analysis difficult. Our aim was to explore the difference in {sup 18}F-FDG uptake kinetics between tumour and inflammatory cells. To this end, we investigated VX2 tumour lesions and inflammatory lesions in rabbits. Methods: Six rabbits with VX2 tumour cells transplanted into one forelimb muscle and inflammatory lesions induced by turpentine oil in the contralateral forelimb were scanned for 60 minutes post {sup 18}F-FDG injection. Imaging data was analyzed with the standard 2-tissue-compartment model. Parameters, VB, Ki, K1, k2, k3, k4, were compared between tumour and inflammatory lesions. SUV and dual time scan methods were also compared in the experiment. Results: Time activity curves of VX2 tumour lesions showed a characteristic pattern of gradually increasing {sup 18}F-FDG uptake up to 60 min, whereas, {sup 18}F-FDG uptake in inflammatory lesions increased more slowly than in tumours. Parameters estimated from the uptake process showed that forward transport constant, K1, and influx constant, Ki, values in VX2 tumour lesions (0.186 {+-} 0.053 and 0.048 {+-} 0.014, respectively) was significantly higher than that in inflammatory lesions (0.129 {+-} 0.024 and 0.022 {+-} 0.007, respectively) (p < 0.05). In contrast, mean values of VB, k2, k3 and k4 derived from VX2 tumours were not significantly different from that of inflammatory lesions. SUVs at 60 minutes post {sup 18}F-FDG injection were also significantly higher in the VX2 tumor lesions than in the inflammatory lesions. Retention index (RI) was not significantly different between VX2 tumours and inflammatory lesions (1.134 {+-} 0.076 vs. 1.060 {+-} 0.058, p > 0.05). Conclusion: Different kinetic parameters (Ki, K1, k3) exist between inflammatory and tumour lesions. (orig.)

  7. Experimental study on the rim-enhancing lesion of rabbit brain abscess : MR imaging and histopathologic correlation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hee Jung; Suh, Soo Jhi; Kim, Sang Pyo; Joo, Yang Goo; Zeon, Seok Kil; Woo, Seong Ku [Keimyung Univ. School of Medicine, Taegu (Korea, Republic of)

    1996-11-01

    To evaluate on the basis of histopathologic carrelation the MR findings of mature brain abscess in the rabbit, with particular attention to rim-enhancing lesions. The evolution of abscess formation was obtained by the direct inoculation of Staphylococcus aureus into the gray-white matter junctions of the brains of 16 rabbits. The stages of brain abscesses were divided into four : early cerebritis (days 1 to 5 after inoculation of the organism);late cerebritis (days 6 to 14);early capsular (days 16 to 21);and late capsular (days 22 to 28). The available MR images showed 14 cases at the stage of early cerebritis, seven at the late cerebritis stage, three at the early capsular, and one at the late capsular stage. According to the known pathology of brain abscesses and on the basis of both MR imaging and histopathologic findings, the lesions were grouped according to whether they were found in the central necrotic, border, or peripheral zone. We analyzed the patterns of rim-enhancement (completeness of the rim, thickness, and margin) and the signal intensities of the abscess walls on MR images at each stage. Histopathologic correlation was performed in one case of each stage. We evaluated the presence or absence and degree of infiltration by inflammatory granulation tissue, microhemorrhage, reticulin, collagen, and hemosiderin of the abscess walls. Rim-enhancing lesions were present in three of 14 cases at the late cerebritis stage, in all three cases at the early capsular, in one at the late capsular, but in none at the early cerebritis stage. The enhancing pattern of the late cerebritis stage was irregular-margined incomplete rim-enhancement, with irregular thickness of the abscess walls (3/3). The enhancing pattern of the capsular stages was well-defined, complete rim-enhancement with uniform thickness of the abscess walls (3/4). The signal intensities of the abscess walls at the late cerebritis and early capsular stages were variable. The late capsular stage ws

  8. Experimental study on the rim-enhancing lesion of rabbit brain abscess : MR imaging and histopathologic correlation

    International Nuclear Information System (INIS)

    To evaluate on the basis of histopathologic carrelation the MR findings of mature brain abscess in the rabbit, with particular attention to rim-enhancing lesions. The evolution of abscess formation was obtained by the direct inoculation of Staphylococcus aureus into the gray-white matter junctions of the brains of 16 rabbits. The stages of brain abscesses were divided into four : early cerebritis (days 1 to 5 after inoculation of the organism);late cerebritis (days 6 to 14);early capsular (days 16 to 21);and late capsular (days 22 to 28). The available MR images showed 14 cases at the stage of early cerebritis, seven at the late cerebritis stage, three at the early capsular, and one at the late capsular stage. According to the known pathology of brain abscesses and on the basis of both MR imaging and histopathologic findings, the lesions were grouped according to whether they were found in the central necrotic, border, or peripheral zone. We analyzed the patterns of rim-enhancement (completeness of the rim, thickness, and margin) and the signal intensities of the abscess walls on MR images at each stage. Histopathologic correlation was performed in one case of each stage. We evaluated the presence or absence and degree of infiltration by inflammatory granulation tissue, microhemorrhage, reticulin, collagen, and hemosiderin of the abscess walls. Rim-enhancing lesions were present in three of 14 cases at the late cerebritis stage, in all three cases at the early capsular, in one at the late capsular, but in none at the early cerebritis stage. The enhancing pattern of the late cerebritis stage was irregular-margined incomplete rim-enhancement, with irregular thickness of the abscess walls (3/3). The enhancing pattern of the capsular stages was well-defined, complete rim-enhancement with uniform thickness of the abscess walls (3/4). The signal intensities of the abscess walls at the late cerebritis and early capsular stages were variable. The late capsular stage ws

  9. Does Artificial Ascites Induce the Heat-Sink Phenomenon during Percutaneous Radiofrequency Ablation of the Hepatic Subcapsular Area?: an in vivo Experimental Study Using a Rabbit Model

    OpenAIRE

    Kim, Young-sun; Rhim, Hyunchul; Choi, Dongil; Lim, Hyo K.

    2009-01-01

    Objective To evaluate the effect of the heat-sink phenomenon induced by artificial ascites on the size of the ablation zone during percutaneous radiofrequency (RF) ablation of the hepatic subcapsular area in an in vivo rabbit model. Materials and Methods A total of 21 percutaneous rabbit liver RF ablations were performed with and without artificial ascites (5% dextrose aqueous solution). The rabbits were divided into three groups: a) control group (C, n = 7); b) room temperature ascites group...

  10. Brachytherapy with Iodine-125 seeds strand for treatment of main portal vein tumor thrombi: an experimental study in a rabbit model

    OpenAIRE

    Zhang, Wen; Luo, Jianjun; Liu, Qingxin; Ma, Jingqin; Qu, Xudong; Yang, Minjie; Yan, Zhiping; Wang, Jianhua

    2016-01-01

    This study aims to establish an animal model of implanted main portal vein tumor thrombus (MPVTT) and to evaluate safety and efficacy of brachy therapy with Iodine-125 (125I) seeds strand to treat MPVTT of rabbit. VX2 tumor thrombus was implanted in main portal vein (MPV) of 32 New Zealand white rabbits. These rabbits were randomly divided into treatment group (Group T, T1-T16) and control group (Group C, C1-C16). 125I seeds and blank seeds strand were implanted in MPV of rabbits in Group T a...

  11. Invasive and Ultrasound Based Monitoring of the Intracranial Pressure in an Experimental Model of Epidural Hematoma Progressing towards Brain Tamponade on Rabbits

    Directory of Open Access Journals (Sweden)

    Konstantinos Kasapas

    2014-01-01

    Full Text Available Introduction. An experimental epidural hematoma model was used to study the relation of ultrasound indices, namely, transcranial color-coded-Doppler (TCCD derived pulsatility index (PI, optic nerve sheath diameter (ONSD, and pupil constriction velocity (V which was derived from a consensual sonographic pupillary light reflex (PLR test with invasive intracranial pressure (ICP measurements. Material and Methods. Twenty rabbits participated in the study. An intraparenchymal ICP catheter and a 5F Swan-Ganz catheter (SG for the hematoma reproduction were used. We successively introduced 0.1 mL increments of autologous blood into the SG until the Cushing reaction occurred. Synchronous ICP and ultrasound measurements were performed accordingly. Results. A constant increase of PI and ONSD and a decrease of V values were observed with increased ICP values. The relationship between the ultrasound variables and ICP was exponential; thus curved prediction equations of ICP were used. PI, ONSD, and V were significantly correlated with ICP (r2=0.84±0.076, r2=0.62±0.119, and r2=0.78±0.09, resp. (all P<0.001. Conclusion. Although statistically significant prediction models of ICP were derived from ultrasound indices, the exponential relationship between the parameters underpins that results should be interpreted with caution and in the current experimental context.

  12. Transauricular embolization of the rabbit coronary artery for experimental myocardial infarction: comparison of a minimally invasive closed-chest model with open-chest surgery

    Directory of Open Access Journals (Sweden)

    Katsanos Konstantinos

    2012-02-01

    Full Text Available Abstract Introduction To date, most animal studies of myocardial ischemia have used open-chest models with direct surgical coronary artery ligation. We aimed to develop a novel, percutaneous, minimally-invasive, closed-chest model of experimental myocardial infarction (EMI in the New Zealand White rabbit and compare it with the standard open-chest surgical model in order to minimize local and systemic side-effects of major surgery. Methods New Zealand White rabbits were handled in conformity with the "Guide for the Care and Use of Laboratory Animals" and underwent EMI under intravenous anesthesia. Group A underwent EMI with an open-chest method involving surgical tracheostomy, a mini median sternotomy incision and left anterior descending (LAD coronary artery ligation with a plain suture, whereas Group B underwent EMI with a closed-chest method involving fluoroscopy-guided percutaneous transauricular intra-arterial access, superselective LAD catheterization and distal coronary embolization with a micro-coil. Electrocardiography (ECG, cardiac enzymes and transcatheter left ventricular end-diastolic pressure (LVEDP measurements were recorded. Surviving animals were euthanized after 4 weeks and the hearts were harvested for Hematoxylin-eosin and Masson-trichrome staining. Results In total, 38 subjects underwent EMI with a surgical (n = 17 or endovascular (n = 21 approach. ST-segment elevation (1.90 ± 0.71 mm occurred sharply after surgical LAD ligation compared to progressive ST elevation (2.01 ± 0.84 mm;p = 0.68 within 15-20 min after LAD micro-coil embolization. Increase of troponin and other cardiac enzymes, abnormal ischemic Q waves and LVEDP changes were recorded in both groups without any significant differences (p > 0.05. Infarct area was similar in both models (0.86 ± 0.35 cm in the surgical group vs. 0.92 ± 0.54 cm in the percutaneous group;p = 0.68. Conclusion The proposed model of transauricular coronary coil embolization avoids

  13. CLINICAL OBSERVATION ABOUT THE EFFECT OF BLOOD-LETTING OF JING-POINTS ON CEREBRAL BLOOD FLOW IN STROKE PATIENTS AT THE EARLY STAGE AND EXPERIMENTAL STUDY ON ITS MECHANISMS IN THE RABBIT

    Institute of Scientific and Technical Information of China (English)

    WANG Xiuyun; REN Shusheng; GUO Yi; ZHOU Guoping; ZHOU Zhiliang; PAN Rongqing; XU Tangping; LI Qing; WANG Xin; REN Huanzhong

    2002-01-01

    In this paper,the authors sum their research resuits about the effect of blood-letting of Jing(Well)-point on cerebral blood flow both in stroke patients and in experimental cerebral ischemia,cerebral hematoma and hypertension rabbits.In 30cases of stroke (cerebral hemorrhage and cerebral infarction)patients,blood flow state of the anterior cerebral artery(ACA),middle cerebral artery(MCA)and the posterior cerebral artery (PCA), and the blood flow velocity of the bilateral vertebral artery (VA)and the basil artery(BA)are determined before and afterpricking blood of the Twelve Jing-points.In experimental cerebral ischernia (by occlusion of the common carotid ertery) rabbits ,cerebral hematoma model rabbits and intravenous injection of noradrenaline induced hypertension rabbits, rheoencephalogram(REC) is detected before and after blood letting of the twelve"Jing -points.In these 30stroke patients,ultrasound Doppler examination's results show that in 22 cases (73.33%) whose blood flow velocity decreases,after blood-letting of the 12 Jing-points, it ncreases significantly(P< 0.01); in the rest 8 cases (26.67%) whose blood flow velocity speeds up,after treatment,it decreases evidently(P<tly (P< 0.01), showing a good dual-directional regulative effect of blood -letting therapy.In experimental cerebral ischemia rabbits,cerebral hematoma rabbits and hypertension rabbits whose REG lowers in the amplitude apparently ( P < 0.01 ), after blood letting stimulation of the 12 Jing-points, it increases at different degrees.Three patterns of stimulation as blood letting stimulation, pain stimulation and Jing-point stimulation, also the 3factors of blood-letting,may contribute to their effect on improvement of the cerebral blood flow.Somatic affterent nerve,sympathetic nerve of the vasular wall,central cholinergic nerve(M receptors)and adrenergic nerve (α receptors) participate in the effect of blood letting on cerebral blood flow.

  14. Vaccination with a mutated variant of human Vascular Endothelial Growth Factor (VEGF) blocks VEGF-induced retinal neovascularization in a rabbit experimental model.

    Science.gov (United States)

    Morera, Yanelys; González, Rafael; Lamdan, Humberto; Pérez, Lincidio; González, Yorlandis; Agüero, Judith; Castro, Jorge; Romero, Juan C; Etchegoyen, Ana Yansy; Ayala, Marta; Gavilondo, Jorge V

    2014-05-01

    Vascular Endothelial Growth Factor (VEGF) is a key driver of the neovascularization and vascular permeability that leads to the loss of visual acuity of eye diseases like wet age-related macular degeneration, diabetic macular edema, and retinopathy of premature. Among the several anti-VEGF therapies under investigation for the treatment of neovascular eye diseases, our group has developed the vaccine candidate CIGB-247-V that uses a mutated form of human VEGF as antigen. In this work we evaluated if the vaccine could prevent or attenuate VEGF-induced retinal neovascularization in the course of a rabbit eye neovascularization model, based on direct intravitreal injection of human VEGF. Our experimental findings have shown that anti-VEGF IgG antibodies induced by the vaccine were available in the retina blood circulation, and could neutralize in situ the neovascularization effect of VEGF. CIGB-247-V vaccination proved to effectively reduce retinal neovascularization caused by intravitreal VEGF injection. Altogether, these results open the way for human studies of the vaccine in neovascular eye syndromes, and inform on the potential mechanisms involved in its effect. PMID:24675387

  15. Neuropatogênese experimental da infecção pelo herpesvírus bovino tipo 5 em coelhos Experimental neuropathogenesis of bovine herpesvirus 5 infection in rabbits

    Directory of Open Access Journals (Sweden)

    Eduardo Furtado Flores

    2009-01-01

    estudar diversos aspectos da infecção pelo BoHV-5.Several aspects of the biology of bovine herpesvirus 5 (BoHV-5 have been studied in rabbits, which develop acute infection and neurological disease upon experimental inoculation. The acute infection is followed by the establishment of latent infection, which can be naturally or artificially reactivated. The first experiments in rabbits established a protocol for virus inoculation and monitoring the infection, and characterized the main virological, clinical and pathological aspects of the acute infection. The pathogenesis of acute infection, from the initial viral replication at site of inoculation, pathways and kinetics of viral transport to the brain, distribution and virus replication in the central nervous system (CNS, cellular and tissue tropism, clinical signs and CNS pathology have been extensively studied using this animal model. Subsequently, several biological and molecular aspects of latent BoHV-5 infection have also been elucidated upon inoculation of rabbits. Rabbits have also been used to investigate the phenotype (neuroinvasiveness, neurogrowth of field isolates and recombinant vaccine candidates, protection by passive immunity, vaccine protection, the efficacy of anti-viral drugs and support therapies for neurological disease. This animal model was also used to investigate the origin and distribution of electric impulses involved in seizures - a hallmark of BoHV-5 induced neurological infection - and also to test the efficacy of anti-convulsivants. In spite of the possible differences between rabbits and cattle - the natural host of the virus - the observations taken from this experimental model have greatly contributed to the knowledge of the biology of BoHV-5 infection. The present article presents a review of the main published and unpublished results and observations by our group, comprising more than a decade of studies on the pathogenesis of BoHV-5 infection in the rabbit model.

  16. Usefulness of high-resolution sonography in early diagnosis of rabbit clonorchiasis

    International Nuclear Information System (INIS)

    To determine the role of high-resolution sonography in the early diagnosis of experimentally induced clonorchiasis in rabbits. We performed sonographic examination weekly in 22 lightly-infected rabbits (10 rabbits infected with 10 metacercariae, 6 rabbits infected with 20 metacercariae, and 6 rabbits infected with 40 metacercariae), and 10 heavily-infected rabbits (500 metacercariae). The sonographic criterion of diagnosis with dilatation of the intrahepatic ducts. We sacrificed lightly-infected rabbits and counted numbers of adult worms of clonorchis sinensis 9 weeks after infection. Sonographic abnormalities were found 3 weeks after infection in 2 lightly-infected rabbits and 5 heavily-infected rabbits. On sonography at 9 weeks after infection, we observed dilatation of the intrahepatic ducts in 11 (65%) of 17 lightly-infected rabbits and all of 10 heavily-infected rabbits. High-resolution sonography is very useful in early diagnosis of rabbits clonorchiasis.

  17. Influence of inducible cross-resistance to macrolides, lincosamides, and streptogramin B-type antibiotics in Enterococcus faecium on activity of quinupristin-dalfopristin in vitro and in rabbits with experimental endocarditis.

    OpenAIRE

    Fantin, B.; Leclercq, R.; Garry, L; Carbon, C

    1997-01-01

    The influence of inducible cross-resistance to macrolides, lincosamides, and streptogramin B (MLS(B)) type antibiotics (inducible MLS(B) phenotype) on the activity of quinupristin-dalfopristin was investigated against Enterococcus faecium in vitro and in rabbits with experimental endocarditis. In vitro, quinupristin-dalfopristin displayed bacteriostatic and bactericidal activities against a MLS(B)-susceptible strain similar to those against two strains with the inducible MLS(B) phenotype. In ...

  18. Autologous grafting of extraocular muscles: experimental study in rabbits Transplante autólogo de musculatura ocular extrínseca: estudo experimental em coelhos

    Directory of Open Access Journals (Sweden)

    Jorge Meireles-Teixeira

    2005-06-01

    Full Text Available PURPOSE: To evaluate the feasibility of autologous extraocular muscle grafting as a type of muscle expansion surgery. METHODS: The left superior rectus muscle of twenty-nine rabbits was resected and this fragment was attached to the endpoint of the respective right superior rectus (test group. Thereafter, the superior rectus of the left eye was reattached to the sclera (control group. Both groups were examined during different postoperative periods in order to assess their outcomes. RESULTS: The presence of hyperemia was slightly more frequent in the grafted group. Secretion and muscle atrophy were negligible in both groups. Fibrosis was greater in grafted animals. These muscles were weaker than the control muscles, although the force required to split muscular parts was always greater than the physiological one. CONCLUSIONS: This surgical technique was reliable and useful if one intends to achieve muscle expansion without the intrinsic risks of dealing with heterologous/artificial materials.OBJETIVO: Avaliar a viabilidade do uso de segmentos de músculos oculares extrínsecos como expansores de tendões musculares. MÉTODOS: Vinte e nove coelhos tiveram seu músculo reto superior esquerdo ressecado e o fragmento de cada um foi transplantado para o reto superior contralateral (grupo-teste. Então, o reto superior esquerdo foi reinserido na esclera (grupo-controle. Os animais foram então examinados em diversos períodos pós-operatórios, até os seus sacrifícios, para que se avaliasse o desenrolar dessa técnica cirúrgica. RESULTADOS: A hiperemia foi maior entre os testes. A secreção e a atrofia muscular foram mínimas nos dois grupos. Houve maior presença de fibrose no grupo-teste, mas não tão expressiva a ponto de inviabilizar os efeitos da cirurgia. Esses músculos também se romperam mais facilmente do que os do grupo-controle, porém, a força de rompimento foi sempre bem maior do que aquela presente numa contração muscular normal

  19. Effect of arterial perfusion of 3-bromopyruvate on transplanted rectal tumors:an experimental study in rabbits%动脉灌注3-溴丙酮酸对兔移植性直肠肿瘤的作用

    Institute of Scientific and Technical Information of China (English)

    翁文采; 张文俊; 王峰; 梁文波; 高宏波; 李成刚; 艾琦; 宋吉慧

    2015-01-01

    目的:观察动脉灌注3-溴丙酮酸(3-BrPA)对兔移植性直肠肿瘤的作用效果。方法将60只移植有直肠肿瘤的新西兰大白兔随机分为低、中、高剂量治疗组及生理盐水对照组,每组各15只。对低、中、高剂量组实验兔分别经导管于肠系膜后动脉灌注0.5 mmol/L、1.0 mmol/L、2.0 mmol/L浓度的3-BrPA各10 ml;对照组灌注等量生理盐水。4 d后活体解剖取出直肠肿瘤,镜下观察肿瘤细胞坏死程度并计算坏死率,评估各浓度3-BrPA对肿瘤的作用效果。结果60只实验兔完成直肠肿瘤移植、动脉灌注实验,镜下实验兔肿瘤细胞均有不同程度损坏。低剂量组Ⅰ级坏死3只,Ⅱ级坏死11只,Ⅲ级坏死1只,治疗有效率为6.7%;中剂量组Ⅱ级坏死2只,Ⅲ级坏死10只,Ⅳ级坏死3只,治疗有效率为86.6%;高剂量组Ⅲ级坏死2只,Ⅳ级坏死13只,治疗有效率为100%;对照组Ⅰ级坏死15只。中、高剂量组Ⅲ、Ⅳ级肿瘤坏死率、治疗有效率、4级肿瘤坏死水平比较差异有统计学意义(P<0.05),3-BrPA治疗作用明显,而正常肠组织无损伤。结论动脉灌注3-BrPA治疗兔移植性直肠肿瘤有一定疗效,高浓度剂量组肿瘤坏死率和治疗有效率最高,疗效显著。%Objective To investigate the effect of 3-bromopyruvate (3-BrPA) on transplanted rectal tumors in experimental rabbit models. Methods A total of 60 New Zealand white rabbits with transplanted rectal tumor were randomly and equally divided into low-dose (0.5 mmol/L), medium-dose (1.0 mmol/L), high-dose (2.0 mmol/L) treatment groups and saline control group with 15 rabbits in each group. Arterial perfusion of 10 ml 3-BrPA with concentration of 0.5 mmol/L, 1.0 mmol/L and 2.0 mmol/L via caudal mesenteric artery was respectively employed for the rabbits of the corresponding treatment group; the control group was perfused with equal amounts of saline. Four days later, rectal tumors were removed by vivisection. The

  20. Histologic and histomorphometric evaluation of the effect of lactoferrin combined with anorganic bovine bone on healing of experimentally induced bony defects on rabbit calvaria

    Directory of Open Access Journals (Sweden)

    Mojgan Paknejad

    2012-01-01

    Conclusion: Based on results of this study, the combination of lactoferrin and ABB did not show any significant improvement in bone regeneration compared with ABB alone in surgically induced bony defects in rabbit calvaria.

  1. Experimental Plan for EDF Energy Creep Rabbit Graphite Irradiations- Rev. 2 (replaces Rev. 0 ORNL/TM/2013/49).

    Energy Technology Data Exchange (ETDEWEB)

    Burchell, Timothy D [ORNL

    2014-07-01

    The experimental results obtained here will assist in the development and validation of future models of irradiation induced creep of graphite by providing the following data: Inert creep stain data from low to lifetime AGR fluence Inert creep-property data (especially CTE) from low to lifetime AGR fluence Effect of oxidation on creep modulus (by indirect comparison with experiment 1 and direct comparison with experiment 3 NB. Experiment 1 and 3 are not covered here) Data to develop a mechanistic understanding, including oAppropriate creep modulus (including pinning and high dose effects on structure) oInvestigation of CTE-creep strain behavior under inert conditions oInformation on the effect of applied stress/creep strain on crystallite orientation (requires XRD) oEffect of creep strain on micro-porosity (requires tomography & microscopy) This document describes the experimental work planned to meet the requirements of project technical specification [1] and EDF Energy requests for additional Pre-IE work. The PIE work is described in detail in this revision (Section 8 and 9).

  2. 正常兔眼泪囊急速萎缩的实验研究%Experimental study on rapid atrophy of lacrimal sac in normal rabbit eyes

    Institute of Scientific and Technical Information of China (English)

    高惠娟; 贺忠江; 杨文慧; 牛淑玲; 刘永盛; 胡艳滨

    2013-01-01

    目的 探索一种治疗泪囊炎快速微创方法的实验研究.方法 选择新西兰大白兔7只(14眼),随机分成对照组1只(2眼)、平阳组(6只左眼)、磷霉素葡萄糖组(6只右眼).对白兔泪道按照泪道冲洗操作规范注药.对照组:9 g·L-1生理盐水.平阳组:盐酸平阳霉素1 mg·kg-1、地塞米松10 mg、2 g·L-1利多卡因2 mL、2 mL生理盐水充分混匀.磷霉素葡萄糖组:磷霉素钠粉剂2 g与2.78 mol·L-1葡萄糖20 mL充分混匀,用5 mL注射器抽取4 mL备用.在给药后1周、2周两个不同时间段解剖分离出泪囊并观察其组织病理形态学变化.结果 对照组无明显变化,泪道黏膜为柱状上皮,结构基本正常.给药后1周,平阳组泪道冲洗通畅,泪道黏膜上皮细胞水肿,上皮下少量炎细胞浸润;磷霉素葡萄糖组泪道冲洗不通畅,黏膜上皮水肿,上皮下大量炎细胞浸润;给药后2周,平阳组泪道冲洗通畅,泪道黏膜上皮细胞增生结构紊乱,少量上皮细胞内陷入软组织中,上皮下炎细胞浸润;磷霉素葡萄糖组泪囊组织炎性细胞浸润,黏膜上皮细胞大部分缺失,泪囊腔内可见血液和胶原渗出,泪囊腔狭窄,泪囊明显萎缩,与周围组织未见明显粘连.结论 磷霉素钠葡萄糖泪道注射硬化剂疗法对兔泪道有较好的闭塞作用,2周即可导致泪囊萎缩,效果优于平阳霉素.临床上尤其适用于合并全身并发症无法实施或不愿意手术的老年慢性泪囊炎患者,有着重要的临床应用价值.%Objective To explore the experimental study for a rapid,radical and minimally invasive method for dacryocystitis. Methods Seven New Zealand rabbits (14 eyes) were randomly divided into control group (1 rabbit,2 eyes) ,Pingyang group (6 left eyes) and fosfomycin sodium glucose group (6 right eyes). The different hardeners were injected into rabbit lacrimal duct:The control group was injected with normal sodium (9 g·L-1) ;The Pingyang group with bleomycin

  3. Induction of Asherman's Syndrome in Rabbit

    OpenAIRE

    Bazoobandi, Sanaz; Tanideh, Nader; Rahmanifar, Farhad; Tamadon, Amin; Keshtkar, Mohammadreza; Mehrabani, Davood; Kasraeian, Maryam; Koohi-Hosseinabadi, Omid

    2016-01-01

    Background: Uterine synechiae or Asherman's syndrome is a condition that can cause infertility. The present experimental study was designed to establish the rabbit as an animal model for human Asherman's syndrome using the endometrial curettage. Methods: In an experimental study, female adult rabbits (n=18) were randomly divided into intact and ovariectomized groups. One third of caudal part of both uteri was submitted to traumatic endometrial curettage. One group was simultaneously ovariecto...

  4. Brachytherapy with Iodine-125 seeds strand for treatment of main portal vein tumor thrombi: an experimental study in a rabbit model

    Science.gov (United States)

    Zhang, Wen; Luo, Jianjun; Liu, Qingxin; Ma, Jingqin; Qu, Xudong; Yang, Minjie; Yan, Zhiping; Wang, Jianhua

    2016-01-01

    This study aims to establish an animal model of implanted main portal vein tumor thrombus (MPVTT) and to evaluate safety and efficacy of brachy therapy with Iodine-125 (125I) seeds strand to treat MPVTT of rabbit. VX2 tumor thrombus was implanted in main portal vein (MPV) of 32 New Zealand white rabbits. These rabbits were randomly divided into treatment group (Group T, T1-T16) and control group (Group C, C1-C16). 125I seeds and blank seeds strand were implanted in MPV of rabbits in Group T and C, respectively. Changes of general condition, body weight and blood laboratory examination were monitored at every time point after procedure. 2 weeks later, 8 rabbits of each group were sacrificed for pathologic examination. The rest of rabbits were dissected postmortem, and therapeutic effects were evaluated on basis of multi-detector computed tomography and histopathology. Ki-67 labeling index (Ki-67 LI) and apoptosis index (AI) were compared between two groups. Overall survival period was recorded. At every time point after brachytherapy, more serious weight loss were detected in Group C. Results of liver function tests and blood cells counts showed no significant difference between two groups. Mean volume of tumor tissue within MPV were 565.40 ± 220.90 mm3 in Group T and 2269.90 ± 437.00 mm3 in Group C (P < 0.001). (Ki-67 LI) and AI were (4.14 ± 1.84)% and (6.51 ± 1.92)% in Group T, compared with (33.82 ± 6.07)% and (0.91 ± 0.26)% in Group C, respectively (P < 0.001). Media survival time of rabbits were 39.50 ± 2.37 days in Group T and 27.38 ± 1.22 days in Group C, respectively (P = 0.001). In conclusion, injecting and suspensory fixing VX2 tumor strip into MPV is a reliable method to establish MPVTT animal model. Brachytherapy with 125I seeds strand was safe and effective to treat VX2 tumor strand inoculated in the MPV of rabbit. PMID:27152237

  5. Viral infections of rabbits.

    Science.gov (United States)

    Kerr, Peter J; Donnelly, Thomas M

    2013-05-01

    Viral diseases of rabbits have been used historically to study oncogenesis (e.g. rabbit fibroma virus, cottontail rabbit papillomavirus) and biologically to control feral rabbit populations (e.g. myxoma virus). However, clinicians seeing pet rabbits in North America infrequently encounter viral diseases although myxomatosis may be seen occasionally. The situation is different in Europe and Australia, where myxomatosis and rabbit hemorrhagic disease are endemic. Advances in epidemiology and virology have led to detection of other lapine viruses that are now recognized as agents of emerging infectious diseases. Rabbit caliciviruses, related to rabbit hemorrhagic disease, are generally avirulent, but lethal variants are being identified in Europe and North America. Enteric viruses including lapine rotavirus, rabbit enteric coronavirus and rabbit astrovirus are being acknowledged as contributors to the multifactorial enteritis complex of juvenile rabbits. Three avirulent leporid herpesviruses are found in domestic rabbits. A fourth highly pathogenic virus designated leporid herpesvirus 4 has been described in Canada and Alaska. This review considers viruses affecting rabbits by their clinical significance. Viruses of major and minor clinical significance are described, and viruses of laboratory significance are mentioned. PMID:23642871

  6. The Rabbit Stream Cipher

    DEFF Research Database (Denmark)

    Boesgaard, Martin; Vesterager, Mette; Zenner, Erik

    2008-01-01

    The stream cipher Rabbit was first presented at FSE 2003, and no attacks against it have been published until now. With a measured encryption/decryption speed of 3.7 clock cycles per byte on a Pentium III processor, Rabbit does also provide very high performance. This paper gives a concise...... description of the Rabbit design and some of the cryptanalytic results available....

  7. Experimental model of the formation of pelvic adhesions by videolaparoscopic in female rabbits Modelo experimental de formação de aderências pélvicas por videolaparoscopia em coelhas

    Directory of Open Access Journals (Sweden)

    Rosi Pereira Balbinotto

    2010-02-01

    Full Text Available PURPOSE: To verify the frequency of postsurgical pelvic adhesion formation in an experimental animal model using videolaparoscopy. METHODS: Experimental study in a sample of 11 non-pregnant female rabbits, aged 5 to 7 months. After general anesthesia, access to the abdominal cavity was performed by an open puncture technique, with 10mm optics, placing two other 5 mm trochars under direct visualization, in the iliac fossae. Then a fragment of peritoneum was resected, followed by electrocauterization. In 21 days, the videolaparoscopy was repeated, and adhesion formation and score was looked at, with biopsies at the surgical site. RESULTS: 54 % of adhesion formation was observed, and the median score of adhesions was 6 (minimum of 3 and maximum of 10, all of them found in the bladder and the anterior abdominal wall. CONCLUSION: The method used presents a high frequency of intra-abdominal adhesion formation.OBJETIVO: Verificar a freqüência da formação de aderências pélvicas pós-cirúrgicas, em um modelo experimental animal, por videolaparoscopia. MÉTODOS: Estudo experimental, em uma amostra de 11 coelhas, não prenhas, com idade entre cinco e sete meses. Após anestesia geral, o acesso da cavidade abdominal foi efetuado por técnica de punção aberta, com óptica de 10 mm, colocando-se outros dois trocateres de 5 mm, sob visão direta, nas fossas ilíacas. Realizou-se, então, ressecção de fragmento de peritônio, seguida de cauterização com eletrocautério. Em 21 dias, foi repetida a videolaparoscopia, verificando-se a formação e escore de aderências e realizando-se biópsias do local da cirurgia. RESULTADOS: Observou-se 54,5% de formação de aderências, sendo o escore total mediano de aderências seis (mínimo de três e máximo de 10, todas encontradas na bexiga e na parede abdominal anterior. CONCLUSÃO: O procedimento utilizado apresentou alta freqüência de formação de aderências intra-abdominais.

  8. Efficacy of Ampicillin plus Arbekacin in Experimental Rabbit Endocarditis Caused by an Enterococcus faecalis Strain with High-Level Gentamicin Resistance

    OpenAIRE

    Kak, Vivek; Donabedian, Susan M.; Zervos, Marcus J.; Kariyama, Reiko; Kumon,Hiromi; Chow, Joseph W.

    2000-01-01

    Enterococcus faecalis LC40 is an ampicillin-susceptible clinical isolate with high-level gentamicin resistance due to the aac(6′)-Ie-aph(2")-Ia aminoglycoside resistance gene. The combination of ampicillin plus arbekacin reduced mean bacterial vegetation counts significantly more than ampicillin alone or ampicillin plus gentamicin in a rabbit model of aortic-valve endocarditis caused by E. faecalis LC40.

  9. Converging Stereotactic Radiotherapy Using Kilovoltage X-Rays: Experimental Irradiation of Normal Rabbit Lung and Dose-Volume Analysis With Monte Carlo Simulation

    International Nuclear Information System (INIS)

    Purpose: To validate the feasibility of developing a radiotherapy unit with kilovoltage X-rays through actual irradiation of live rabbit lungs, and to explore the practical issues anticipated in future clinical application to humans through Monte Carlo dose simulation. Methods and Materials: A converging stereotactic irradiation unit was developed, consisting of a modified diagnostic computed tomography (CT) scanner. A tiny cylindrical volume in 13 normal rabbit lungs was individually irradiated with single fractional absorbed doses of 15, 30, 45, and 60 Gy. Observational CT scanning of the whole lung was performed every 2 weeks for 30 weeks after irradiation. After 30 weeks, histopathologic specimens of the lungs were examined. Dose distribution was simulated using the Monte Carlo method, and dose-volume histograms were calculated according to the data. A trial estimation of the effect of respiratory movement on dose distribution was made. Results: A localized hypodense change and subsequent reticular opacity around the planning target volume (PTV) were observed in CT images of rabbit lungs. Dose-volume histograms of the PTVs and organs at risk showed a focused dose distribution to the target and sufficient dose lowering in the organs at risk. Our estimate of the dose distribution, taking respiratory movement into account, revealed dose reduction in the PTV. Conclusions: A converging stereotactic irradiation unit using kilovoltage X-rays was able to generate a focused radiobiologic reaction in rabbit lungs. Dose-volume histogram analysis and estimated sagittal dose distribution, considering respiratory movement, clarified the characteristics of the irradiation received from this type of unit.

  10. Usefulness of non-ionic, low osmolar contrast agent (Reyon Iopamidol 300 and Iversion 320) for IVU (intravenous urography), angiography and CT : an experimental study in normal rabbits

    International Nuclear Information System (INIS)

    To compare the diagnostic efficacy of domestically synthesized contrast materials (Reyon Iopamidol 300 and Iversion 320) used for IVU, CT, and abdominal angiography in normal rabbits with that of previously used products (Ultravist 300 and Optiray 320). A total of 60 rabbits were divided into six groups of ten. In the first group, IVU was performed using Reyon Iopamidol 300 and Ultravist 300, while in the second, abdominal angiography involved the use of Iversion 320 and Optiray 320. For three-phase spiral CT, Reyon Iopamidol 300 was used for group 3, Ultravist 300 for group 4, Iversion 320 for group 5, and Optiray 320 for group 6. The degree of contrast enhancement seen on aortography and IVU was evaluated subjectively by two radiologists who reached a consensus while unaware of the names of the contrast materials. On CT, attenuation expressed in Hounsfield Units (HU) was measured using the regions of interest (ROIs) facility in each anatomic region during the early and delayed phases. Adverse events including death occurred during the investigation. There were no significant differences in the degree of contrast enhancement between Reyon Iopamidol 300 and Ultravist 300, as used for IVU, and between Iversion 320 and Optiray 320, as used for angiography. With two exceptions, abdominal CT revealed no significant differences in the degree of contrast enhancement of most anatomic structure. The exceptions were greater enhancement of the portal vein with Ultravist 300 than with Reyon Iopamidol 300 during the delayed phase, and greater enhancement of the aorta with Optiray 320 than with Iversion 320 during the delayed phase. In no rabbit was adverse reaction observed. For IVU, angiography and abdominal CT in normal rabbits, Reyon Iopamidol 300 and Iversion 320 provide contrast enhancement comparable to that provided by Ultravist 300 and Optiray 320. In order to evaluate the diagnostic efficacy of these new agents in diseased tissue and in human subjects, further studies

  11. Paclitaxel-Coated Balloons: Investigation of Drug Transfer in Healthy and Atherosclerotic Arteries – First Experimental Results in Rabbits at Low Inflation Pressure

    OpenAIRE

    Stolzenburg, Nicola; Breinl, Janni; Bienek, Stephanie; Jaguszewski, Milosz; Löchel, Melanie; Taupitz, Matthias; Speck, Ulrich; Wagner, Susanne; Schnorr, Jörg

    2016-01-01

    Purpose Beyond antiproliferative properties, paclitaxel exhibits anti-inflammatory activity, which might be beneficial in the local treatment of nonocclusive coronary artery disease. Paclitaxel release and tissue concentrations after paclitaxel-coated balloon treatment using different pressures have not been investigated so far. The aim of the study was to investigate in an atherosclerotic rabbit model whether drug transfer from paclitaxel-coated balloons into the vessel wall is affected by t...

  12. The selection of window width and levels for measuring the airway dimensions with spiral CT scan: an experimental study in Japanese white big-ear rabbits

    International Nuclear Information System (INIS)

    Objective: To investigate the optimal window width and window level for measuring the airway dimensions with spiral CT scan in Japanese white big-ear rabbits so as to lay the foundation for airway stenting in animal experiments. Methods: Multi-slice spiral CT scanning of cervico-thoracic region was performed in 30 healthy adult Japanese white big-ear rabbits, the anteroposterior and transversal diameter of the thoracic trachea, the anteroposterior diameter of the right and left bronchus were measured with lung window, mediastinum window and special fat window separately. The revealing rate of the tracheal wall and the measuring results in different windows and levels were recorded and compared with the anatomical data. The differences of the relevant data were statistically analyzed. Results: With lung window, the tracheal wall was well demonstrated, but the relevant data were smaller than that with mediastinum window. With mediastinum window, the data were bigger and the tracheal wall border appeared blurred. The results obtained with fat window were close to the actual anatomical data. Conclusion: For accurately measuring the anteroposterior and transversal diameter of the thoracic trachea in Japanese white big-ear rabbits with multi- slice spiral CT scan, fat window should be adopted, which is helpful for the preparation of tracheal and bronchial stents. (authors)

  13. Correlation between neurohypophyseal vasopressin content and signal intensity on T{sub 1}-weighted magnetic resonance images. An experimental study of vasopressin depletion model using dehydrated rabbits

    Energy Technology Data Exchange (ETDEWEB)

    Kurokawa, Hiroaki; Nakano, Yoshihisa; Ikeda, Koshi; Tanaka, Yoshimasa [Kansai Medical Univ., Moriguchi, Osaka (Japan); Fujisawa, Ichiro

    1998-06-01

    We investigated the correlation between the signal intensity on T{sub 1}-weighted MR images and vasopressin (VP) content in the posterior pituitary lobe. Fourteen rabbits were studied. There were 12 water-deprived rabbits (48, 72, 96, 120, 144 and 168 hours: 2 each) and 2 controls. Sagittal T{sub 1}-weighted SE (spin-echo) MR images were obtained before and after dehydration. The signal intensity ratio of the posterior pituitary lobe to the pons was correlated with the VP content in the posterior lobe as measured by radioimmunoassay. Before water deprivation, high signal intensity in the posterior lobe was demonstrated clearly in all 14 rabbits. After water deprivation, the hyperintense signal gradually decreased and became indistinguishable from anterior lobe in four animals. The mean signal intensity ratio before water deprivation was 1.55{+-}0.12 (mean{+-}SD) and after water deprivation, gradually decreased over time and reached to 1.19 after 168 hours of water deprivation. Pituitary VP content and concentration decreased in parallel with the signal intensity ratio of the posterior pituitary. Significantly correlation was observed between the signal intensity ratio and VP concentration of posterior pituitary (r=0.809, p<0.001) . In conclusion, the results indicate that the signal intensity ratio on T{sub 1}-weighted image may reflect a indicator of pituitary VP content and thus may enable evaluation of disorders of water metabolism. (author)

  14. Does Artificial Ascites Induce the Heat-Sink Phenomenon during Percutaneous Radiofrequency Ablation of the Hepatic Subcapsular Area?: an in vivo Experimental Study Using a Rabbit Model

    International Nuclear Information System (INIS)

    To evaluate the effect of the heat-sink phenomenon induced by artificial ascites on the size of the ablation zone during percutaneous radiofrequency (RF) ablation of the hepatic subcapsular area in an in vivo rabbit model. A total of 21 percutaneous rabbit liver RF ablations were performed with and without artificial ascites (5% dextrose aqueous solution). The rabbits were divided into three groups: a) control group (C, n = 7); b) room temperature ascites group (R, n = 7); and c) warmed ascites group (W, n = 7). The tip of a 1 cm, internally cooled electrode was placed on the subcapsular region of the hepatic dome via ultrasound guidance, and ablation was continued for 6 min. Changes in temperature of the ascites were monitored during the ablation. The size of the ablation zones of the excised livers and immediate complications rates were compared statistically between the groups (Mann-Whitney U test, Kruskal-Wallis test, linear-by-linear association, p = 0.05). One rabbit from the 'W' group expired during the procedure. In all groups, the ascites temperatures approached their respective body temperatures as the ablations continued; however, a significant difference in ascites temperature was found between groups 'W' and 'R' throughout the procedures (39.2± 0.4 .deg. C in group W and 33.4±4.3 .deg. C in group R at 6 min, p = 0.003). No significant difference was found between the size of the ablation zones (782.4±237.3 mL in group C, 1,172.0±468.9 mL in group R, and 1,030.6±665.1 mL in group W, p = 0.170) for the excised liver specimens. Diaphragmatic injury was identified in three of seven cases (42.9%) upon visual inspection of group 'C' rabbits (p = 0.030). Artificial ascites are not likely to cause a significant heat-sink phenomenon in the percutaneous RF ablation of the hepatic subcapsular region

  15. Effects of Ascorbic Acid, Alpha-Tocopherol and Allopurinol on Ischemia-Reperfusion Injury in Rabbit Skeletal Muscle: An Experimental Study

    Directory of Open Access Journals (Sweden)

    Bilgehan Erkut

    2007-01-01

    Full Text Available Purpose: Ischemia reperfusion injury to skeletal muscle, following an acute arterial occlusion is important cause of morbidity and mortality. The aim of the present study was to determine and evaluate the effects of ascorbic acide, alpha-tocopherol and allopurinol on ischemia reperfusion injury in rabbit skeletal muscle.Methods: Forty-eight New Zealand white rabbits, all male, weighing between 2.5 to 3.0 (mean 2.8 kg, were used in the study. They were separated into four groups. Group I was the control group without any drugs. The other groups were treatment groups (groups II, III, and IV. Group II rabbits administrated 50 mg/kg ascorbic acide and 100 mg/kg alpha-tocopherol 3 days prior to ischemia, group III rabbits received 50 mg/kg allopurinol 2 days prior to ischemia, and group IV rabbits were administrated both 50 mg/kg ascorbic acide, 100 mg/kg alpha-tocopherol 3 days prior to ischemia and 50 mg/kg allopurinol 2 days prior to ischemia. Two hours ischemia and 2 hours reperfusion were underwent to the treatment groups. At the end of the reperfusion periods, muscle samples were taken from rectus femoris muscle for determination of superoxide dismutase, catalase and glutathione peroxidase activities as antioxidant enzymes, and malondialdehyde as an indicator of lipid peroxidation and xanthine oxidase levels as source hydroxyl radical. Besides, histopathological changes (edema, inflammation, ring formation and splitting formation were evaluated in the muscle specimens. Results: In the treatment groups; superoxide dismutase (U/mgprotein, catalase (U/mgprotein, and glutathione peroxidise (U/mgprotein levels increased, malondialdehyde (nmol/mgprotein and xanthine oksidase (mU/mgprotein levels decreased compared to control I (p < 0.05. Increase of superoxide dismutase, catalase, and glutathione peroxidase levels were the highest and decrease of malondialdehyde and xanthine oxidase levels were the highest in group IV compared to groups II and III

  16. Does Artificial Ascites Induce the Heat-Sink Phenomenon during Percutaneous Radiofrequency Ablation of the Hepatic Subcapsular Area?: an in vivo Experimental Study Using a Rabbit Model

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young Sun; Rhim, Hyun Chul; Choi, Dong Il; Lim, Hyo K. [Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

    2009-02-15

    To evaluate the effect of the heat-sink phenomenon induced by artificial ascites on the size of the ablation zone during percutaneous radiofrequency (RF) ablation of the hepatic subcapsular area in an in vivo rabbit model. A total of 21 percutaneous rabbit liver RF ablations were performed with and without artificial ascites (5% dextrose aqueous solution). The rabbits were divided into three groups: a) control group (C, n = 7); b) room temperature ascites group (R, n = 7); and c) warmed ascites group (W, n = 7). The tip of a 1 cm, internally cooled electrode was placed on the subcapsular region of the hepatic dome via ultrasound guidance, and ablation was continued for 6 min. Changes in temperature of the ascites were monitored during the ablation. The size of the ablation zones of the excised livers and immediate complications rates were compared statistically between the groups (Mann-Whitney U test, Kruskal-Wallis test, linear-by-linear association, p = 0.05). One rabbit from the 'W' group expired during the procedure. In all groups, the ascites temperatures approached their respective body temperatures as the ablations continued; however, a significant difference in ascites temperature was found between groups 'W' and 'R' throughout the procedures (39.2+- 0.4 .deg. C in group W and 33.4+-4.3 .deg. C in group R at 6 min, p = 0.003). No significant difference was found between the size of the ablation zones (782.4+-237.3 mL in group C, 1,172.0+-468.9 mL in group R, and 1,030.6+-665.1 mL in group W, p = 0.170) for the excised liver specimens. Diaphragmatic injury was identified in three of seven cases (42.9%) upon visual inspection of group 'C' rabbits (p = 0.030). Artificial ascites are not likely to cause a significant heat-sink phenomenon in the percutaneous RF ablation of the hepatic subcapsular region

  17. Allergy to Rabbits. 1

    International Nuclear Information System (INIS)

    Investigations have been carried out into the presence of antibody light chains in rabbit allergenic extracts and the interference in RAST and crossed-radioimmunoelectrophoresis (XRIE) caused by antibodies directed against them. A ''non-specific'' uptake of radioactivity in XRIE has been demonstrated to be caused by direct cross-linking of the 125I rabbit anti-human IgE by the sheep antibodies in the immunoprecipitate of rabbit light chains. Preincubation with normal rabbit serum blocked this direct uptake of the labelled antibody and enabled specific IgE uptake on the light chains to be demonstrated for rabbit allergic sera. Verification of the allergenicity of the light chains was obtained from a specific light chain RAST. Elution from a Sephacryl S-200 gel filtration column indicated a MW of approx. 50Kd and confirmation of the components as light chain dimers, not Fab fragments, was obtained by allotyping for loci present on heavy chains and light chains in the Fab region. Light chains were detected in urine from rabbits of all ages and in an extract of dust collected in a rabbit housing area. No background staining was observed in XRIE using rabbit antisera, either with rabbit allergic sera with specific IgE or with a human serum containing specific IgG antibodies to rabbit IgG. This latter serum also showed no evidence of uptake on all immunoprecipitates in systems using rabbit antisera, and did not give false positive RAST results when the labelled rabbit anti-human IgE contained unlabelled rabbit IgG. Those sera with specific IgE to light chains showed no uptake in XRIE using rabbit antisera, indicating that the IgE was possibly specific for epitopes revealed by the dissociation on the whole IgG molecule. (author)

  18. Cyclic pneumatic rabbit with program control

    International Nuclear Information System (INIS)

    The structure of cyclic pneumatic rabbit with automatic program control applied at the IRT 2000 reactor for fission products investigation is described. The pneumatic rabbit is mounted on the base of a standard 25 mm diameter vertical experimental channel of the IRT-2000 reactor. A duralumin pipe with 12.5 mm inner diameter serves as a sample feeding channel. For accurate fixing the time of start and finish of sample irradiation the pneunatic rabbit is provided with a limit switch. Principle electric equipment schemes of the pneumatic rabbit are presented and the construction of the limit switch meant for operation at the neutron flux density of about 1013 neutron/cm2xs is given. The base of electric equipment of the pneumatic rabbit is a programmer mounted at RC-circuits. Step-by-step selectors and polarized relays serve as executive elements. The working body of the pneumatic rabbit is air of pneumatic or compressed pipe-line helium. By means of a described pneumatic rabbit one can irradiate a sample in the time interval from 3 to 1000 s, perform four measurements in the interval from 4 to 1000 s as well as realize a reverse discharge of the irradiated sample into the core. The number of cycles can vary from 1 to 100. The time for sample supply of 25g mass from the reactor core to the detector (distance about 30 m) constitutes 3 s

  19. Perfil del peso corporal en la isquemia mesentérica aguda: Estudio experimental en conejos Nueva Zelanda Body weight profile in acute mesenteric ischemia: Experimental study in New-Zealand rabbits

    Directory of Open Access Journals (Sweden)

    J. F. Guijarro Escribano

    2005-12-01

    final del estudio un peso porcentual significativamente inferior que el alcanzado por la serie I. En los procesos de isquemia reperfusión mesentérica experimental animal, la pérdida de peso porcentual inicial en el postoperatorio, está influenciada no sólo por el tiempo de isquemia, sino por las manipulaciones pre y postoperatorias; en cambio, la pérdida de peso al final del estudio es más acentuada, cuanto mayor es el tiempo de isquemia.Introduction and objectives: There are few investigation studies that relate acute mesenteric ischemia and body weight in animal experimentation. The aim is to studying whether initial weight loss is related to the magnitude of the aggression induced by clamps of the superior mesenteric artery for 30 and 60 minutes, and whether reperfusion ischemia may hinder weight recovery in surviving animals at the end of 11 weeks of experimental work with New Zealand rabbits through a valid experimental model. Materials and method: 80 animals (rabbits were distributed in four series of 20 each one: series I (control, animals were weighed for 11 weeks; series II (simulated surgery; series III (mesenteric ischemia for 30 minutes; series IV (mesenteric ischemia for 60 minutes. We induced ischemia by clamping the superior mesenteric artery. Animals from series II, III, and IV were weighed 24 hours before the surgical procedure and weekly after surgery along their survival, until completing 11 weeks. For weight analysis, an ANOVA test was used by confronting the percentage weight variation according to the series. All animals were necropsied to know the cause of death and histological lesions of the intestinal mucosa. Results: Series I had a linear weight increase until the end of the observation period. Series II, III and IV had a significant initial decrease of the percentage weight during the first post-surgical week, with a recovery towards the end of the study, but significantly lower as compared to the control series. Significant

  20. Circulating antitoxin in rabbits after ingestion of diphtheria toxoid.

    OpenAIRE

    Peri, B A; Rothberg, R M

    1981-01-01

    Immune responses following antigen ingestion vary from stimulation to suppression depending on animal species, antigen, and experimental protocol. Young adult rabbits were given either 0.02% diphtheria toxoid or 0.1% bovine serum albumin in drinking water for 10-day periods, a protocol previously found to immunize human infants fed bovine serum albumin. Specific serum antibody was detected by radioimmunoassay in 10 of 13 rabbits fed diphtheria toxoid for 10 days and 11 of 13 rabbits fed bovin...

  1. Comparative study of bridge plate associated to the intramedullary pin and the dynamic compression plate on the experimental osteotomy fixation of femoral in rabbits (Oryctolagus cuniculus). Clinical, radiographic, histological and scintigraphy analyses

    International Nuclear Information System (INIS)

    The objectives were to benchmark and monitor the fracture healing of femoral osteotomy in rabbits after fixation with dynamic compression plate and bridge plate associated with intramedullary pin. Were used 14 New Zealand rabbits, four months old with mean weight of 3.5 pounds, from the Experimental Farm Professor Helio Barbosa of the Veterinary School of Minas Gerais Federal Univ. (UFMG). The animals were randomly divided into two groups (I and II). All rabbits underwent osteotomy across the middle third of right femur. In the group I was made to fix the osteotomy with the bridge plate (BP) associated with intramedullary pin, introduced the technique of minimal invasion. In group II, we used dynamic compression plate (DCP) via the conventional approach. Both groups were evaluated clinical, radiographic, histologic and scintigraphic findings. Clinical assessments were performed weekly until the 12th postoperative week and radiographic examinations were performed before, immediately after, at 15, 30, 45, 60 and 90 days. The bone scintigraphy were performed before and at 20, 50 and 90 days after surgery to monitor the bone metabolism qualitatively and quantitatively. Observed perfusion and bone healing process. After 90 days of study, there was histopathologic evaluation of the osteotomized area and the insertion of screws. In the region of the osteotomy was observed predominance of trabecular bone in group I and group II, the predominance of bone osteons, compatible with the original bone. On insertion of the screws did not differ between groups and there was cortical discontinuity, little necrosis and local hemorrhage. The two types of fixation have led to consolidation within the scheduled period, maturing in early fixation with DCP fixation compared with BP. Scintigraphy demonstrated by the indices of activity and image characteristics, the process of bone healing was significantly greater in animals undergoing early fixation with DCP. (author)

  2. Comparison of the diagnosis of plain radiography ultrasonography and magnetic resonance imaging in early diagnosis of acute osteomyelitis experimentally formed on rabbits

    International Nuclear Information System (INIS)

    Purpose: We have compared the sensitivities of MRI, US and radiography which are some of the radiologic modalities used in the early diagnosis of acute osteomyelitis. Materials and methods: After 1 week care and research on 20 New Zealand rabbits (average weight: 2.5 kg) in animal laboratory, study was begun. Six rabbits died after anesthesia and surgical procedure. There were 14 rabbits remaining and 10 of them formed the study group. Four of them were used as the control group. Fresh mouse feces was inoculated into the bone marrow of 20 tibia under general anesthesia and during the following 15 days, MRI, US and radiographic researches were made everyday. Results: According to the results that we got at the end of the 15-day period, MRI was found as the most sensitive method in describing early diagnosis of infection in the bone marrow and also in showing the extension of the infection to the soft tissue. Although US showed the findings later than MRI, it was determined that its sensitivity was less than but near MRI's in detecting subperiosteal fluid in short term period. Radiographic research gives acute osteomyelitis diagnosis latest, so it was determined as the modality that has the lowest sensitivity. Conclusion: MRI which is one of the radiologic modalities among other diagnostic radiologic modalities that can be used in early diagnosis of acute osteomyelitis, has the highest sensitivity. Ultrasonography is the second most sensitive modality. Radiographic analysis has the least sensitivity among other modalities. As a result, first MRI then US and after that plain radiography should be used for the early diagnosis of acute osteomyelitis

  3. Morpho-functional study of ionizing radiation effects on the rabbits` femoral vein; Avaliacao morfofuncional do efeito da radiacao ionizante sobre a veia femoral. Estudo experimental em coelhos

    Energy Technology Data Exchange (ETDEWEB)

    Sakiyama, Mauro Yoshimitsu

    1995-12-31

    In this study we evaluate the effects of the ionizing radiation on the rabbits femoral vein. The samples of femoral vein were obtained from 56 New Zealand rabbits, male with ageing from 90 to 120 days, that were divided into 4 groups of 14 animals: one control group non-irradiated and three animal groups sacrificed 2 days, 14 days and 90 days after irradiation. In the three irradiated rabbits groups, each animal received the total dose 4000 cGy (rads) divided in 10 sessions of 400 cGy, a dose equivalent that utilized on clinical therapeutic. A morpho functional study of vein samples was carried out with: light microscopy: stained by hematoxin - eosin, Masson`s tricromic, and Verhoeff. Immunohistochemical: reactions of immunoperoxidase with monoclonal mouse anti-human endothelial cell factor CD-31 and anti-human Von Willebrand factor (factor VIII), to study the vein endothelium. Histomorphometry of elastic fiber system stained by Weigert`s resorcin-fuchsin with and without prior oxidation with oxone; for the study of mature, elaunin or pre-mature and oxytalan or young elastic fibers. Electronic microscopy: transmission and scanning. With the methodology utilized we observe changes in the femoral vein of the animals submitted to irradiation in relation to the control group, thus described: there is formation of vacuoles between the endothelium and the basal membrane, called sub endothelial vacuoles, in focal areas. The factor VIII and CD-31 endothelial antigens are preserved with no changes in their functions. Focal alterations are present in the endothelial surface with disorder in the setting and orientation of the endothelial cells. there is degeneration of the elastic fibers with significant decrease in their quantity in the stage, 2 days and 14 days after irradiation. There is increase in the quantity of elastic fibers in the late stage, 90 days after irradiation, tending to normality. In this present study, the changes described are not accompanied by venous

  4. Rabbit Models for Studying Human Infectious Diseases.

    Science.gov (United States)

    Peng, Xuwen; Knouse, John A; Hernon, Krista M

    2015-12-01

    Using an appropriate animal model is crucial for mimicking human disease conditions, and various facets including genetics, anatomy, and pathophysiology should be considered before selecting a model. Rabbits (Oryctolagus cuniculus) are well known for their wide use in production of antibodies, eye research, atherosclerosis and other cardiovascular diseases. However, a systematic description of the rabbit as primary experimental models for the study of various human infectious diseases is unavailable. This review focuses on the human infectious diseases for which rabbits are considered a classic or highly appropriate model, including AIDS (caused by HIV1), adult T-cell leukemia-lymphoma (human T-lymphotropic virus type 1), papilloma or carcinoma (human papillomavirus) , herpetic stromal keratitis (herpes simplex virus type 1), tuberculosis (Mycobacterium tuberculosis), and syphilis (Treponema pallidum). In addition, particular aspects of the husbandry and care of rabbits used in studies of human infectious diseases are described. PMID:26678367

  5. Experimental Embolization of Rabbit Renal Arteries to Compare the Effects of Poly L-Lactic Acid Microspheres With and Without Epirubicin Release Against Intraarterial Injection of Epirubicin

    International Nuclear Information System (INIS)

    Purpose: We performed a basic investigation using white rabbits of the sustained release and embolizing effects of poly L-lactic acid microspheres (PLA) to determine their usefulness for chemoembolization.Methods: Fifteen male Japanese white rabbits were used. Sustained release of an embolizing material, EPI-PLA was accomplished with 1 mg of PLA containing 0.03 mg of epirubicin hydrochloride (EPI). Embolization with 50 mg of PLA (total dose of EPI 1.5 mg) was performed after the renal artery of the rabbits was selected (Chemo-TAE group). A group in which a bolus of 1.5 mg EPI alone was injected through the renal artery (TAI group) was established as a control group. Furthermore, a group in which embolization was performed with 50 mg of PLA alone (TAE group) was also established. These three groups, each consisting of five rabbits, were compared.Results: Blood EPI levels were serially measured. The blood EPI level in the TAI group rapidly reached a peak more than 30 min after injection, then decreased to almost zero 24 hr after injection. In the Chemo-TAE group, the blood EPI level was transiently increased 30 min after embolization, but remained low thereafter until 24 hr after embolization. EPI levels in kidney tissue isolated 24 hr after embolization were measured. In the Chemo-TAE group, the tissue EPI level was significantly higher than that in the TAI group. When isolated kidneys were macroscopically and histologically examined, atrophy of the entire embolized kidney, as well as infarction and necrosis in the renal cortex, were observed in both the TAE group and the Chemo-TAE group. However, there were no such findings in the TAI group. The area of the infarction in the renal cortex did not significantly differ between the Chemo-TAE group and the TAE group; however, there was vascular injury in the Chemo-TAE group and none in the TAE group.Conclusion: It was demonstrated that EPI-PLA, a chemoembolizing material, maintained high local concentrations of the

  6. Experimental embolization of rabbit renal arteries to compare the effects of poly L-lactic acid microspheres with and without epirubicin release against ntraarterial injection of epirubicin

    International Nuclear Information System (INIS)

    Purpose: We performed a basic investigation using white rabbits of the sustained release and embolizing effects of poly L-lactic acid microspheres (PLA) to determine their usefulness for chemoembolization.Methods: Fifteen male Japanese white rabbits were used. Sustained release of an embolizing material, EPI-PLA was accomplished with l m g of PLA containing 0.03 mg of epirubicin hydrochloride (EPI). Embolization with 50 mg of PLA (total dose of EPI l.5 mg) was performed after the renal artery of the rabbits was selected (Chemo-TAE group). A group in which a bolus of 1.5 mg EPI alone was injected through the renal artery (TAI group) was established as a control group. Furthermore, a group in which embolization was performed with 50 mg of PLA alone (TAE group) was also established. These three groups, each consisting of five rabbits, were compared.Results: Blood EPI levels were serially measured. The blood EPI level in the TAI group rapidly reached a peak more than 30 min after injection, then decreased to almost zero 24 hr after injection. In the Chemo-TAE group, the blood EPI level was transiently increased 30 min after embolization, but remained low thereafter until 24 hr after embolization. EPI levels in kidney tissue isolated 24 hr after embolization were measured. In the Chemo-TAE group, the tissue EPI level was significantly higher than that in the TAI group. When isolated kidneys were macroscopically and histologically examined, atrophy of the entire embolized kidney, as well as infarction and necrosis in the renal cortex, were observed in both the TAE group and the Chemo-TAE group. However, there were no such findings in the TAI group. The area of the infarction in the renal cortex did not significantly differ between the Chemo-TAE group and the TAE group; however, there was vascular injury in the Chemo-TAE group and none in the TAE group.Conclusion: It was demonstrated that EPI-PLA, a chemo-embolizing material, maintained high local concentrations of the

  7. Local Administration of NF-κ B Decoy Oligonucleotides to Prevent Restenosis after Balloon Angioplasty: An Experimental Study in New Zealand White Rabbits

    International Nuclear Information System (INIS)

    Purpose. To evaluate the efficacy of NF-κ B oligonucleotides (ODN) administered by local administration with the channeled balloon catheter to prevent restenosis after balloon angioplasty in restenotic iliac arteries of New Zealand white rabbits. Materials and Methods. In vitro, 8000 rabbit vascular smooth muscle cells (rVSMC) where transfected with a liposomal carrier (TfX50) with 100 ng of decoy and scrambled ODN. Inhibition of proliferation was measured using a MTT assay after 24 hours in comparison to control. In vivo, 22 male New Zealand White rabbits were fed a 1% cholesterol diet and received denudation of both common iliac arteries with a 3 mm balloon catheter to induce an arterial stenosis. Four weeks after stenosis induction, local application of NF-κ B in two different concentrations (1 μg: n = 14; 10 μg: n = 8) was performed randomly on one common iliac artery. Scrambled oligonucleotides without specific binding capacities were injected into the contralateral side. The channeled balloon catheter allows simultaneous balloon dilation (8 atm) of the stenosis and local application of a drug solution (2 atm). Four weeks after local drug delivery the animals were killed and the vessels were excised and computerized morphometric measurements were performed. Results. NF-κ B decoy ODN but not scrambled ODN inhibited proliferation of rVSMC in vitro. Following local ODN application in the animals, no acute vascular complications were seen. NF-κ B ODN resulted in a statistically non significant reduction of neointimal area compared to the control group. The neointimal area was 0.97 mm2 using 1 μg NF-κ B ODN compared to 0.98 mm2 in the control group. The higher dose resulted in a neointimal area of 0.97 mm2 compared to 1.07mm2 at the control side. Conclusions. Local drug delivery of NF-κ B ODN using the 'channeled balloon' catheter could not reduce neointimal hyperplasia in stenostic rabbit iliac arteries. Application modalities have to be improved to

  8. Experimental study on 13N-NH3 and 99MTc-MIBI myocardial perfusion imaging in rabbits with subacute myocardial infarction of ischemic reperfusion

    International Nuclear Information System (INIS)

    Purpose: To explore the relationship between 13N-NH3 and 99mTc-MIBI myocardial perfusion imaging in rabbits with subacute myocardial infarction of ischemic reperfusion. Methods: Eight male New Zealand White rabbits of which left anterior descending (LAD) coronary arteries were completely occluded for 45 min followed by 7-10 d reperfusion. One week later, the rabbits after an overnight fast were anaesthetized with sodium pentobarbital (30 mg/kg), and LAD arteries were religated for 45 min followed by 2 h reperfusion. Then the animals were positioned on the LS-PET/CT (4 row spirals CT, Discovery GE. US) table. Myocardial blood flows were obtained with 148 MBq 13N-NH3 administered via a marginal ear vein over 20 s. According to PET imaging procedure, PET/CT acquisition of dynamic scans began 5 min after injection and was accomplished within 10 min. Two hours after PET imaging the rabbits were injected with 148 MBq 99mTc-MIBI via a marginal ear vein, 30 min later myocardial perfusion imaging was performed under a single-photon emission computed tomography (SPECT). PET imaging ZOOM value was generally amplified 6 times while that of SPECT was 3 times. Tomographic images along the vertical long, horizontal long and short axes were created. Tomographic reconstruction was then performed by dividing the PET and SPECT image of the LV on a polar map into 9 segments for semi- quantitative analysis. The changes of infarct size were determined by triphenyl tetrazolium chloride (TTC) staining. The ultra-structural damage of myocardial cells in infarct and periphery areas were observed under transmission electron microscope. Results: Infarct size (24.2±1.9)% of LV mass by TTC staining, while (23.7±2.3)% vs. (20.5± 2.5)% (P < 0.001) by 99mTc-MIBI Hawkeye-SPECT and 13N-NH3 PET/CT respectively. Serious myocardial cell damages including myocardial cell denaturalization, texture, and karyolysis in infracted area and myocardial cells swelling in the periphery of infracted area was

  9. Frequency-dependent anti arrhythmic effects if Crataegus monogyna on extracellular field potential recording in experimental model of AF atrioventricular Node of rabbit.

    OpenAIRE

    Vahid Khori; Mohammad Azadbakht; Mohsen Nayebpour; Amirhosean Jamshidi; Mona Pourabouk; Alimohammad Alizadeh; Fakhri Badaghabadi; Shima Changizi; Maryam Rajaei

    2011-01-01

    Introduction: Despite extensive studies about effects of Crataegus monogyna on cardiovascular diseases yet, a few study has been undertaken antiarrhythmic property of this plant. Aims of the present study were: 1) To determine the protective role of methanolic extract of C.monogyna on the rate-dependent model and the concealed conduction . 2) To explore the role of Na+-K+ A TPase in the protective role of C.monogyna.   Methods: In all experiments were used of male New Zealand rabbits (1.5-2kg...

  10. The influence of intravenous laser irradiation of blood on some metabolic and functional parameters in intact rabbits and experimental cerebral ischaemia

    Science.gov (United States)

    Nechipurenko, N.; Vasilevskaya, L.; Musienko, J.; Maslova, G.

    2007-07-01

    It has been studied the intravenous laser irradiation of blood (ILIB) influence with helium-neon laser (HNL) of 630 nm wavelength on some of lipid peroxidation (LPO) and antioxidant system (AOS) findings, aside-base status (ABS) and blood oxygen transport (BOT), state of dermal microhaemodynamics (MGD) in the intact rabbits and after modeling of local ischemia of brain (LIB). Depending on conditions of organism functioning (norm or brain ischaemia) ILIB has resulted in stimulating or normalizing effects on the whole metabolic and microhaemocirculation processes which had been studied during our investigation. It is discussed the mechanisms of pathogenetic directivity of ILIB influence in cerebral ischaemia

  11. Delivery of antifibroblast agents as adjuncts to filtration surgery. Part I--Periocular clearance of cobalt-57 bleomycin in experimental drug delivery: pilot study in the rabbit

    International Nuclear Information System (INIS)

    Antitumor and antifibroblast agents show promise as adjuncts after glaucoma filtration surgery in reducing postoperative scarring and failure. We used nuclear imaging in rabbits to investigate periocular clearance of one such agent (57Co-bleomycin). Sub-Tenon injection was compared to other delivery techniques. Our results showed that a collagen sponge and a silastic disc implant with a microhole prolonged drug delivery when compared to sub-Tenon injection alone or injection with a viscosity enhancing agent (0.5% sodium hyaluronate). We theorize that if an antifibroblast agent can be delivered in small and sustained amounts after filtration surgery, this may prolong bleb longevity and avoid unnecessary drug toxicity

  12. Characteristic analysis on susceptibility weighted imaging of intravitreous foreign body of autologous eyelashes in rabbits

    Institute of Scientific and Technical Information of China (English)

    YANG Yun-jun; CHENG Jing-liang; WANG Juan; ZHANG Yong; LI Hua-li

    2010-01-01

    Objective: To explore the characteristics of susceptibility weighted imaging (SWI) of the intravitreous foreign body of autologous eyelashes in rabbits.Methods: A total of 12 New Zealand white rabbits,either sex, weighing 2.5-3.5 kg, and provided by the Experimental Animal Center of Henan Province were employed in this study. For each rabbit, 5 autologous eyelashes (1 cm in length and 0.2-0.3 mm in diameter) were implanted into the right ocular vitreum, while the left control ocular vitreum received sham operation but nothing was implanted. SWI sequential test was made 2 hours postoperatively. Then the rabbits were killed and the specimens of the vitreous bodies of the rabbits were obtained. Hematoxylin and eosin staining and histological examinations were performed. Results: The autologous eyelashes in 8 ocular vitreums of rabbits showed linear low signal intensity on the magnitude images and susceptibility weighted images, but linear high signal intensity on the phase images. Among the 12experimental rabbits, 5 eyelashes in the right vitreum were completely shown in 3 rabbits, partly shown in 5 rabbits (2eyelashes shown in 3 rabbits and 3 eyelashes shown in 2rabbits), and not shown in 4 rabbits. Conclusions: SWI of the foreign body ofintravitreous autologous eyelashes in rabbits has its own characteristics. The combined application of SWI sequential magnitude images, susceptibility weighted images and phase images is helpful to the detection and diagnosis of intravitreous autologous eyelashes in rabbits.

  13. An initial experimental study-the value of gadolinium-enhanced MRI and delay enhanced MRI in detecting articular cartilage degeneration of the rabbit knee

    International Nuclear Information System (INIS)

    Objective: To explore the appearance and value of early and delaye Gadolinium- enhanced MRI in detecting aricular cartilage generation of rabbit knee. Methods: Twenty adult New Zealand rabbits were randomly divided into five groups (A, B, C, D, E). Intra-articular injection of Papain was performed to establish animal models of different stages of cartilage degeneration in the right knees of A, B, C, D groups, and MR scan was conducted 24 hours,one week one month and three months after the last Papain injection. The knees were scanned bilaterally with T1WI and 3D-FS-SPGR in sagittal plane. The signal intensity ratio between articular cartilage and surrounding soft tissue was measured at plain scan and 0, 2, 4 hours after intravenous injection of gadolinium (Gd-DTPA). The articular cartilage was pathologically examined (HE and alcican blue stain). Results: In 3D-FS-SPGR sequence, it showed significant difference in the SIR between processing side of four groups (F=7.961, P1WI sequence in detecting the change of cartilage signal intensity. (2)SIR on early and delayed Gadolinium-enhanced MRI has the ability in evaluating the early stage change of cartilage degeneration. (authors)

  14. Are percutaneous transgastric biopsies using 14-,16- and 18-G Tru-Cut needles safe? An experimental study in the rabbit

    Energy Technology Data Exchange (ETDEWEB)

    Akan, H.; Incesu, L.; Gunes, M. [Ondokuz Mayis University Medical School, Samsun (Turkey). Department of Radiology; Ozen, N.; Gumus, S. [Ondokuz Mayis University Medical School, Samsun (Turkey). Department of Surgery

    1998-05-01

    We evaluated perforation, peritonitis and bleeding after 14-, 16-, 18-G Tru-Cut needles were passed through the stomach in an animal model. Insertions were performed on seven anesthetized rabbits with 18-,16-and 14-G needles simulating the clinical technique. Diluted contrast medium with methylene blue (60mL) was administered through a nasogastric tube after the biopsy. Computed tomography (CT) scanning of the upper abdomen was performed 10 minutes after the insertion for leakage. The abdominal contents were inspected by laparotomy. There was no contrast medium leakage outside the gastrointestinal tract on CT images. Neither methylene blue nor other stomach content leakage was identified by laparotomy, even with manual squeezing. A tiny spot of blood was observed on the serosal surface of the stomach with the 18-G needle passes (five cases), whereas a relatively large haematoma was present with 14-G needle (four cases). Six of the rabbits survived and one died due to an inadvertant aortic injury. The results of the study show that transgastric route with an 1 8-G cutting needle can be used without fear of peritonitis and bleeding. Copyright (1998) Blackwell Science Pty Ltd 19 refs., 1 tab., 1 fig.

  15. Safety and Biocompatibility of a New High-Density Polyethylene-Based Spherical Integrated Porous Orbital Implant: An Experimental Study in Rabbits

    Directory of Open Access Journals (Sweden)

    Ivan Fernandez-Bueno

    2015-01-01

    Full Text Available Purpose. To evaluate clinically and histologically the safety and biocompatibility of a new HDPE-based spherical porous orbital implants in rabbits. Methods. MEDPOR (Porex Surgical, Inc., Fairburn, GA, USA, OCULFIT I, and OCULFIT II (AJL Ophthalmic S.A., Vitoria, Spain implants were implanted in eviscerated rabbis. Animals were randomly divided into 6 groups (n=4 each according to the 3 implant materials tested and 2 follow-up times of 90 or 180 days. Signs of regional pain and presence of eyelid swelling, conjunctival hyperemia, and amount of exudate were semiquantitatively evaluated. After animals sacrifice, the implants and surrounding ocular tissues were processed for histological staining and polarized light evaluation. Statistical study was performed by ANOVA and Kaplan-Meier analysis. Results. No statistically significant differences in regional pain, eyelid swelling, or conjunctival hyperemia were shown between implants and/or time points evaluated. However, amount of exudate differed, with OCULFIT I causing the smallest amount. No remarkable clinical complications were observed. Histological findings were similar in all three types of implants and agree with minor inflammatory response. Conclusions. OCULFIT ophthalmic tolerance and biocompatibility in rabbits were comparable to the clinically used MEDPOR. Clinical studies are needed to determine if OCULFIT is superior to the orbital implants commercially available.

  16. Rabbit haemorrhagic disease: are Australian rabbits (Oryctolagus cuniculus) evolving resistance to infection with Czech CAPM 351 RHDV?

    Science.gov (United States)

    Elsworth, P G; Kovaliski, J; Cooke, B D

    2012-11-01

    Rabbit haemorrhagic disease is a major tool for the management of introduced, wild rabbits in Australia. However, new evidence suggests that rabbits may be developing resistance to the disease. Rabbits sourced from wild populations in central and southeastern Australia, and domestic rabbits for comparison, were experimentally challenged with a low 60 ID50 oral dose of commercially available Czech CAPM 351 virus - the original strain released in Australia. Levels of resistance to infection were generally higher than for unselected domestic rabbits and also differed (0-73% infection rates) between wild populations. Resistance was lower in populations from cooler, wetter regions and also low in arid regions with the highest resistance seen within zones of moderate rainfall. These findings suggest the external influences of non-pathogenic calicivirus in cooler, wetter areas and poor recruitment in arid populations may influence the development rate of resistance in Australia. PMID:22244198

  17. 显微CT对兔骨质疏松模型的实验研究%Experimental study on a rabbit osteoporosis model with micro-CT

    Institute of Scientific and Technical Information of China (English)

    徐铮; 李冠武; 汤光宇; 刘勇; 张晓

    2011-01-01

    目的 探讨去势法联合皮质激素快速诱导兔骨质疏松模型椎体骨小梁Micro-CT动态变化规律.方法 40只8个月龄雌性大白兔随机分为假手术组(A组,n=20)及去势+肌注甲强龙组(B组,n=20).术后第2、4、8、10周行血清骨转化标志物检测及离体腰5椎体Micro-CT扫描.每组在每个时间点动物数5只.结果 两组vBMD在术后第8周开始,差异有统计学意义,而BVF、Tb.N、Conn.D、Tb.Sp、SMI及Tb.Th分别在术后4、8周开始,差异有统计学意义.B组术后TRAP-5b迅速增高,至第4周达峰值,术后第4、8、10周,两组TRAP-5b差异有统计学意义;而BALP缓慢逐渐增高,至第8周才达峰值,术后第8、10周,两组BALP差异有统计学意义.结论 去卵巢+肌注甲强龙可在8周内快速建立兔OP模型,骨微结构衰败早于vBMD变化而与骨转化标志物同步.%Objective To observe the morphological characteristics of osteoporosis ( OP) in a overiectomized rabbit model with micro-computed tomography (micro-CT). Methods Forty 8-month-old, skeletally mature, female New Zealand white rabbits were randomly allocated into two groups; group B (n = 20) underwent bilateral overiectomy ( OVX) combined with intramuscular injection of methylprednisolone hemisuccinate(MPH); group A(n =20) were used as sham operation controls. Five rabbits from both groups were sacrificed at 2, 4, 8 and 10 weeks after OVX, respectively. The serum bone metabolism markers were measured and micro-CT scanning of L5 vertebral body was performed. Results The bone mineral density (BMD) of group B at 8 and 10 weeks after OVX was significantly decreased compared to that of group A(P<0.01). The microstructural parameters BVF, Conn. D, Tb. N, SMI, Tb. Sp and Tb. Th in group B were significantly lower than those in group A at 4 and 8 weeks after OVX. Serum TRAP-5b in group B was significantly increased at 4, 8 and 10weeks after OVX compared to group A(P <0. 01); while serum BALP was significantly increased

  18. A new safety concern for glaucoma treatment demonstrated by mass spectrometry imaging of benzalkonium chloride distribution in the eye, an experimental study in rabbits.

    Directory of Open Access Journals (Sweden)

    Françoise Brignole-Baudouin

    Full Text Available We investigated in a rabbit model, the eye distribution of topically instilled benzalkonium_(BAK chloride a commonly used preservative in eye drops using mass spectrometry imaging. Three groups of three New Zealand rabbits each were used: a control one without instillation, one receiving 0.01%BAK twice a day for 5 months and one with 0.2%BAK one drop a day for 1 month. After sacrifice, eyes were embedded and frozen in tragacanth gum. Serial cryosections were alternately deposited on glass slides for histological (hematoxylin-eosin staining and immunohistological controls (CD45, RLA-DR and vimentin for inflammatory cell infiltration as well as vimentin for Müller glial cell activation and ITO or stainless steel plates for MSI experiments using Matrix-assisted laser desorption ionization time-of-flight. The MSI results were confirmed by a round-robin study on several adjacent sections conducted in two different laboratories using different sample preparation methods, mass spectrometers and data analysis softwares. BAK was shown to penetrate healthy eyes even after a short duration and was not only detected on the ocular surface structures, but also in deeper tissues, especially in sensitive areas involved in glaucoma pathophysiology, such as the trabecular meshwork and the optic nerve areas, as confirmed by images with histological stainings. CD45-, RLA-DR- and vimentin-positive cells increased in treated eyes. Vimentin was found only in the inner layer of retina in normal eyes and increased in all retinal layers in treated eyes, confirming an activation response to a cell stress. This ocular toxicological study confirms the presence of BAK preservative in ocular surface structures as well as in deeper structures involved in glaucoma disease. The inflammatory cell infiltration and Müller glial cell activation confirmed the deleterious effect of BAK. Although these results were obtained in animals, they highlight the importance of the safety

  19. EFEITO DO LEVAMISOL E DO EXTRATO ETANÓLICO DE FOLHAS DE Momordica Charantia SOBRE A DERMATOFITOSE EXPERIMENTAL EM COELHOS EFFECT OF THE LEVAMISOLE AND THE ETHANOLIC EXTRACT OF Momordica charantia LEAVES ON THE EXPERIMENTAL DERMATOPHYTOSIS IN RABBITS

    Directory of Open Access Journals (Sweden)

    Olivardo Facó

    2007-07-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do levamisol e do extrato etanólico das folhas de Momordica charantia na dermatofitose experimental. Para tanto, coe¬lhos jovens, Nova Zelândia, machos, divididos em grupos, receberam, por via oral, durante quinze dias consecutivos Tween 20 (1%, controle; n=5, levamisol (25 mg/Kg/PV; n=4 ou extrato etanólico de M. charantia (EE, 10 mg/Kg/PV, n=6 a partir do 15º dia inoculação por Microsporum canis. Foram realizadas as contagens total e diferencial de leucócitos do sangue periférico, cultivo do raspado de pele e avaliação histopatológica das lesões. O levamisol e o EE reduziram os escores de avaliação histológica das lesões provocadas pelo M. canis e não induziram modificação dos leucócitos circulantes. O tratamento com levamisol provocou alterações na pele infectada em relação ao controle (p<0,01, mas não diferiu do tratamento com EE, o qual não diferiu do controle que recebeu o veículo. Os resultados demonstraram que o levamisol teve melhor desempenho no tratamento da dermatofitose, enfatizando seu potencial imu¬nomodulador, enquanto o EE de M. charantia apresentou um efeito bastante promissor, indicando uma alternativa de tratamento da dermatofitose provocada pelo M. canis. PALAVRAS-CHAVES: Cucurbitaceae, imunomodulação, levamisol, Microsporum canis, Momordica charantia. The aim of this work was to evaluate the effect of levamisole and ethanolic extract of Momordica charantia leaves on experimental dermatophytosis. Young rabbits, New Zealand, males, were divided in groups that received by via oral during 15 days Tween 20 (1%, control; n=5, levamisole (25 mg/kg/LP; n=4 or ethanolic extract of M. charantia leaves (EE, 10 mg/kg/LP; n=6, beginning at 15th day of Microsporum canis inoculation. Total and differential blood circulating leukocyte counts, cultive of skin and histopatological evaluation of the lesions were realized. Levamisole and EE reduced the

  20. Allergy to rabbits. II

    International Nuclear Information System (INIS)

    Quantitative immunoelectrophoretic techniques have been used to study the antigenic components found in extracts of dust collected from rabbit housing areas. To determine the possible source of these antigens, comparisons have been made to rabbit saliva, urine, fur and dander. Specific antisera for the rabbit extracts were raised in guinea pigs, One major component of the dust (Ag Rl) was also found in large amounts in saliva, slightly less in fur and in only minimal amounts in urine and dander. Crossed radioimmunoelectrophoresis (XRIE) of the dust, performed with sera from 14 rabbit allergic individuals who were RAST positive to rabbit saliva, urine and dust identified four IgE-binding constituents. Individual responses varied but all sera reacted with Ag Rl, identifying this as a major rabbit allergen. Dust RAST inhibition studies with rabbit dust, saliva and urine indicated saliva to be closely related to the dust. Ag Rl is a glycoprotein which appears to be very heterogeneous in nature. It produced a broad biphasic precipitin peak on immunoelectrophoresis and eluted from Sephacryl S-200 gel filtration over the molecular weight range 30-50 Kd, although a molecular weight of 17 Kd was indicated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) and gradient gel electrophoresis. The RAST inhibition results and the antigenic similarity of saliva to the dust suggest this to be the most likely sorce of the major rabbit allergen, Ag Rl. (author)

  1. Experimental study on effect of Qingfeiyin granule in rabbit ear acne model%清肺饮颗粒对家兔实验性耳痤疮影响的研究

    Institute of Scientific and Technical Information of China (English)

    郑慕雄; 王建中; 郭义龙; 陈玉兴

    2011-01-01

    目的 建立家兔实验性耳痤疮模型,观察复方清肺饮颗粒治疗痤疮的作用.方法 家兔左耳内侧涂浓煤焦油溶液0.5ml,1次/d,连续2周,建立耳痤疮动物模型.耳痤疮模型建立成功后将家兔随机分为清肺饮颗粒大、中、小剂量组、解毒痤疮丸组、模型实验组、正常对照组,分别灌饲清肺饮颗粒、解毒痤疮丸和蒸馏水2周,在造模前后及药物治疗前后,取家兔左右耳廓相同部位耳片,称重后计算耳片重量差值,光镜下观察耳廓组织病变.结果 外涂煤焦油1周后,兔耳表面粗糙,增厚,可见粉刺、丘疹,组织学改变与人类痤疮相似;给药2周后,清肺饮颗粒不同剂量组和解毒痤疮丸组均可不同程度抑制痤疮模型家兔耳重量的增加,并明显改善耳痤疮炎性病理变化.结论 清肺饮颗粒对家兔实验性耳痤疮有治疗作用,可用于痤疮的防治.%Objective To establish an experimental animal model of acne and investigate the therapeutic effect of Qingfeiyin granule for acne.Methods Experimental rabbit ear acne model was induced by external application of coal tar.Then the rabbits were randomly divided into high dose,medium dose and low dose Qingfeiyin granule group,Jie Du ance-pill group and negative control group,after that medicines above-mentioned were given respectively.The weight of the acne model ear was measured and histopathologic examination was performed by light microscope.Results One week after external application of coal tar,the surface of rabbit ears became rough and thick,comedones and papules could be seen there.The pathological changes of the lesions in rabbit ears were similar to that of human acne.Two weeks after medicine given,the comedones and papules were extinct in Qingfeiyin granule groups and Jie Du acne-pill group,and corresponding pathological changes were detected by light microscope.Conclusion Qingfeiyin granule has significant experimental anti-acne effects.

  2. Reduction of Intimal Hyperplasia with Re-188-labeled Stents in a Rabbit Model at 7 and 26 Weeks: An Experimental Study

    International Nuclear Information System (INIS)

    The aim of this study was to analyze the feasibility of 188Re-labeled stents to reduce neointimal formation in a rabbit atherosclerosis model and to test the long-term effects at 7 and 26 weeks. Fifty-nine male New Zealand White rabbits were fed a 0.5% cholesterol diet for 4 weeks before balloon angioplasty and insertion of Palmaz stents in the infrarenal aorta. The animals were sacrificed 7 and 26 weeks after stent implantation. Control stents were compared with 188Re stents: (dose 1) 11.3 ± 1.8 MBq; (dose 2) 37.3 ± 4.2 MBq, and (dose 3) 80.1 ± 7.8 MBq. Each activity group consisted of a short-term (7 weeks) and a long-term group (26 weeks), resulting in a total of eight study groups. No thrombotic occlusion was observed. The neointimal formation in the control group was 2.11 [95% confidence interval (CI): 0.68-6.52] mm2 at 7 weeks and 2.10 (0.62-7.11) at 26 weeks. In the treatment groups, neointima reduction was detectable at 7 weeks [dose 1: 0.33 (0.09-1.22) mm2; dose 2: 0.17 (0.05-0.57) mm2; dose 3: 0.03 (0.01-0.13) mm2]. After 26 weeks, a catch-up of neointimal formation in the radioactive groups was most obvious in the low-dose group [dose 1: 0.80 (0.28-2.29) mm2; dose 2: 0.18([0.06-0.52) mm2; dose 3: 0.50 (0.17-1.42) mm2]. Compared to the long-term control group, neointimal reduction was still >60%. No induction of neointimal formation was observed at the edges of the stents. Radiation resulted in delayed re-endothelialization. 188Re stents were capable to reduce intimal hyperplasia and did not cause thrombosis. The edge effect, which was the major limitation of 32P stents, was not observed in 188Re stents

  3. Reduction of intimal hyperplasia with Re-188-labeled stents in a rabbit model at 7 and 26 weeks: an experimental study.

    Science.gov (United States)

    Tepe, Gunnar; Dietrich, Tobias; Grafen, Franziska; Brehme, Ute; Muschick, Peter; Dinkelborg, Ludger M; Greschniok, Annette; Claussen, Claus D; Duda, Stephan H

    2005-01-01

    The aim of this study was to analyze the feasibility of (188)Re-labeled stents to reduce neointimal formation in a rabbit atherosclerosis model and to test the long-term effects at 7 and 26 weeks. Fifty-nine male New Zealand White rabbits were fed a 0.5% cholesterol diet for 4 weeks before balloon angioplasty and insertion of Palmaz stents in the infrarenal aorta. The animals were sacrificed 7 and 26 weeks after stent implantation. Control stents were compared with (188)Re stents: (dose 1) 11.3 +/- 1.8 MBq; (dose 2) 37.3 +/- 4.2 MBq, and (dose 3) 80.1 +/- 7.8 MBq. Each activity group consisted of a short-term (7 weeks) and a long-term group (26 weeks), resulting in a total of eight study groups. No thrombotic occlusion was observed. The neointimal formation in the control group was 2.11 [95% confidence interval (CI): 0.68--6.52] mm(2) at 7 weeks and 2.10 (0.62--7.11) at 26 weeks. In the treatment groups, neointima reduction was detectable at 7 weeks [dose 1: 0.33 (0.09--1.22) mm(2); dose 2: 0.17 (0.05--0.57) mm(2); dose 3: 0.03 (0.01--0.13) mm(2)]. After 26 weeks, a catch-up of neointimal formation in the radioactive groups was most obvious in the low-dose group [dose 1: 0.80 (0.28--2.29) mm(2); dose 2: 0.18([0.06--0.52) mm(2); dose 3: 0.50 (0.17--1.42) mm(2)]. Compared to the long-term control group, neointimal reduction was still >60%. No induction of neointimal formation was observed at the edges of the stents. Radiation resulted in delayed re-endothelialization. (188)Re stents were capable to reduce intimal hyperplasia and did not cause thrombosis. The edge effect, which was the major limitation of (32)P stents, was not observed in (188)Re stents. PMID:16059762

  4. Effect of hepatic blood flow alteration on the therapeutic effect of cryoablation in VX2 hepatic tumor rabbit: an experimental study

    International Nuclear Information System (INIS)

    Objective: To investigate the effect of alteration of blood flow in the hepatic artery on the therapeutic effect of cryoablation in VX2 hepatic tumor rabbit model. Methods: Thirty rabbits with VX2 hepatic tumor were divided into three groups according to hepatic artery blood flow: complete occlusion of the hepatic artery(group A), partial occlusion of the hepatic artery (group B), and no occlusion of the hepatic artery (group C). With conventional CT scan and perfusion scan, the values of blood flow (BF) and blood volume(BV) of VX2 tumor were computed and the differences among the three groups were analyzed. After cryoablation, the animals were euthanized and the livers were removed. The hepatic tissue from the cryoablation area and surrounding area underwent both methyl thiazolyl tetrazolium (MTY) diaphorase staining and triphenyl tetrazolium chloride (TTC) staining. The gross pathology and histopathological changes were observed. Results: (1)The BF and BV in the three groups were: (7.23 + 2. 15 ) ml·100 g-1·min-1 and (1.63±0.52) ml/100 g in group A; (32.65±6.12) ml·100 g-1·min-1 and (9.32±2.63) ml/100 g in group B; (61.34±12.15) ml·100 g-1·min-1 and (17.51± 3.14) ml/100 g in group C, respectively. There were significant differences among the three groups in the BF and BV (F value was 452.16 and 421.33 in the BF and BV, respectively, P <0.01); (2) The maximum diameter of cryoablation-induced necrosis was (2.3±0.3)cm in group A, (1.5±0.2) cm in group B, and (0.8±0.1) cm in group C, respectively. The difference was significant among the groups (F value was 315.32,P <0.01). (3) There were well-defined frozen areas, bordering areas and normal surrounding areas in MTT staining. In group C, positive staining around some blood vessels could be seen. Conclusion: Alteration of the blood flow in the hepatic artery can affect the cryoablation efficacy. With the decrease of hepatic artery blood flow, the efficacy of cryoablation on liver tumor increased

  5. Rabbit meat consumption in Kenya

    OpenAIRE

    Mailu, S.K; Muhammad, L; Wanyoike, M.M; Mwanza, R.N.

    2012-01-01

    A survey was undertaken in 7 counties in Kenya covering a total of 300 rabbit farmers. Another 100 non rabbit keeping farmers was similarly interviewed for comparison purposes. Questions on the survey instrument sought to identify consumption patterns of rabbit meat among the sample farmers. Results were subjected to chi square test for association in an attempt to identify characteristics of respondents that might be pointers to rabbit meat consumption. Education, the number of rabbits kept—...

  6. Coccidian and nematode infections influence prevalence of antibody to myxoma and rabbit hemorrhagic disease viruses in European rabbits.

    Science.gov (United States)

    Bertó-Moran, Alejandro; Pacios, Isabel; Serrano, Emmanuel; Moreno, Sacramento; Rouco, Carlos

    2013-01-01

    The interaction among several parasites in European rabbits (Oryctolagus cuniculus) is crucial to host fitness and to the epidemiology of myxomatosis and rabbit hemorrhagic disease. These diseases have caused significant reductions in rabbit populations on the Iberian Peninsula. Most studies have focused on the epidemiology and pathogenesis of these viruses individually, and little is known about interactions between these viruses and other parasites. Taking advantage of an experimental restocking program in Spain, the effects of coccidian and nematode infections on the probability of having detectable antibody to myxoma and rabbit hemorrhagic disease viruses were tested in European wild rabbits. For 14 mo, we monitored rabbit abundance and parasite loads (coccidia and nematodes) in three reintroduced rabbit populations. While coccidian and nematode loads explained seasonal antibody prevalences to myxoma virus, the pattern was less clear for rabbit hemorrhagic disease. Contrary to expectations, prevalence of antibody to myxoma virus was inversely proportional to coccidian load, while nematode load seemed to play a minor role. These results have implications for viral disease epidemiology and for disease management intended to increase rabbit populations in areas where they are important for ecosystem conservation. PMID:23307367

  7. Field and experimental data indicate that the eastern cottontail (Sylvilagus floridanus) is susceptible to infection with European brown hare syndrome (EBHS) virus and not with rabbit haemorrhagic disease (RHD) virus.

    Science.gov (United States)

    Lavazza, Antonio; Cavadini, Patrizia; Barbieri, Ilaria; Tizzani, Paolo; Pinheiro, Ana; Abrantes, Joana; Esteves, Pedro J; Grilli, Guido; Gioia, Emanuela; Zanoni, Mariagrazia; Meneguz, Pier; Guitton, Jean-Sébastien; Marchandeau, Stéphane; Chiari, Mario; Capucci, Lorenzo

    2015-01-01

    The eastern cottontail (Sylvilagus floridanus) is an American lagomorph. In 1966, it was introduced to Italy, where it is currently widespread. Its ecological niche is similar to those of native rabbits and hares and increasing overlap in distribution brings these species into ever closer contact. Therefore, cottontails are at risk of infection with the two lagoviruses endemically present in Italy: Rabbit Haemorrhagic Disease virus (RHDV) and European Brown Hare Syndrome Virus (EBHSV). To verify the susceptibility of Sylvilagus to these viruses, we analyzed 471 sera and 108 individuals from cottontail populations in 9 provinces of north-central Italy from 1999 to 2012. In total, 15-20% of the cottontails tested seropositive for EBHSV; most titres were low, but some were as high as 1/1280. All the cottontails virologically tested for RHDV and EBHSV were negative with the exception of one individual found dead with hares during a natural EBHS outbreak in December 2009. The cottontail and the hares showed typical EBHS lesions, and the EBHSV strain identified was the same in both species (99.9% identity). To experimentally confirm the diagnosis, we performed two trials in which we infected cottontails with both EBHSV and RHDV. One out of four cottontails infected with EBHSV died of an EBHS-like disease, and the three surviving animals developed high EBHSV antibody titres. In contrast, neither mortality nor seroconversion was detected after infection with RHDV. Taken together, these results suggest that Sylvilagus is susceptible to EBHSV infection, which occasionally evolves to EBHS-like disease; the eastern cottontail could therefore be considered a "spill over" or "dead end" host for EBHSV unless further evidence is found to confirm that it plays an active role in the epidemiology of EBHSV. PMID:25828691

  8. RabbitMQ essentials

    CERN Document Server

    Dossot, David

    2014-01-01

    This book is a quick and concise introduction to RabbitMQ. Follow the unique case study of Clever Coney Media as they progressively discover how to fully utilize RabbitMQ, containing clever examples and detailed explanations.Whether you are someone who develops enterprise messaging products professionally or a hobbyist who is already familiar with open source Message Queuing software and you are looking for a new challenge, then this is the book for you. Although you should be familiar with Java, Ruby, and Python to get the most out of the examples, RabbitMQ Essentials will give you the push y

  9. Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands.

    Science.gov (United States)

    Burt, Sara A; Veltman, Jorg; Hakze-van der Honing, Renate; Schmitt, Heike; van der Poel, Wim H M

    2016-09-01

    Rabbits have been suggested as a zoonotic source of Hepatitis E virus. Phylogenetic analysis of HEV isolates from farmed, wild and pet rabbits in the Netherlands (23, 0, and 60 % respectively) showed them to be grouped amongst published rabbit HEV sequences and distinct from most human isolates. Dutch rabbits are unlikely to be a zoonotic source. PMID:27147250

  10. Experimental study of bleeding control on liver biopsy in rabbit: N-butyl 2-cyanoacrylate(NBCA) injection and RF electrocauterization

    International Nuclear Information System (INIS)

    To evaluate the hemostatic effect of N-butyl 2-cyanoacrylate(NBCA) injection and RF electrocauterization of the tract after fine needle biopsy of the liver, and the histopathologic changes of the liver. Three lobes of rabbit liver were selected and separately punctured four times with 21 gauge biopsy needles. According to the hemostatic procedure on fine needle biopsy, three groups (1, 2, 3) were found : group 1, in which there was no maneuver for bleeding control, was the control group; group 2, in which NBCA was injected into the puncture tract while slowly removing the needle; group 3, in which RF electrocauterization of the tract was carried out. After completely removing the needle, each group was evaluated for amount of bleeding and histologic change. The amount of bleeding was 0.407gm ±0.245 in group 1, 0.028gm ± 0.036 in group 2 and 0.035gm ±0.028 in group 3. As compared with the control group(group 1), injecting NBCA into the biopsy tract(P=0.0002) and RF electrocauterization of the tract(P=0.0003) significantly reduced the amount of bleeding after liver biopsy. The amount of bleeding was not statistically different between group 2 and 3, however (P=0.58). In Group 1, the tract was fully filled with blood. Group 2 showed NBCA embolized in the biopsy tract, adhering to hepatocytes and mixed with blood; small vessels adjacent to the puncture tract were filled with NBCA. Group 3 showed tissue degeneration, including necrosis of hepatocytes, vacuolation and neutrophil infiltration. Injection of NBCA and RF electrocauterization of the tract after puncture of the liver for biopsy efficiently controlled bleeding. In particular, the efficiency of NBCA injection was due to its effect of plugging the tract and causing the embolization of adjacent small vessels. With regard to procedural handling, RF electrocauterization of the tract is superior to injection of NBCA

  11. Rabbit production in Bulgaria

    OpenAIRE

    Dimitrova Ivona; Dimitrov Tz.; Teneva A.; Tzvetkova H.

    2008-01-01

    The aim of the study was to present the situation of rabbit production in Bulgaria. As a whole the rabbit production in Bulgaria is in extensive stage. To change it as intensive or semi intensive it need to improve selection and feeding systems, to concentrate the farm, and to build new more modern farms with control systems of microclimatic parameters and which covered veterinarian requirements.

  12. Novel bocaparvoviruses in rabbits.

    Science.gov (United States)

    Lanave, G; Martella, V; Farkas, S L; Marton, S; Fehér, E; Bodnar, L; Lavazza, A; Decaro, N; Buonavoglia, C; Bányai, K

    2015-11-01

    Bocaparvovirus is a newly established genus within the family Parvoviridae and has been identified as a possible cause of enteric, respiratory, reproductive/neonatal and neurological disease in humans and several animal species. In this study, metagenomic analysis was used to identify and characterise a novel bocaparvovirus in the faeces of rabbits with enteric disease. To assess the prevalence of the novel virus, rectal swabs and faecal samples obtained from rabbits with and without diarrhoea were screened with a specific PCR assay. The complete genome sequence of the novel parvovirus was reconstructed. The virus was distantly related to other bocaparvoviruses; the three ORFs shared 53%, 53% and 50% nucleotide identity, respectively, to homologous genes of porcine bocaparvoviruses. The virus was detected in 8/29 (28%) and 16/95 (17%) samples of rabbits with and without diarrhoea, respectively. Sequencing of the capsid protein fragment targeted by the diagnostic PCR identified two distinct bocaparvovirus populations/sub-types, with 91.7-94.5% nucleotide identity to each other. Including these novel parvoviruses in diagnostic algorithms of rabbit diseases might help inform their potential pathogenic role and impact on rabbit production and the virological profiles of laboratory rabbits. PMID:26383859

  13. 光动力疗法对兔正常鼻黏膜组织结构的影响%Experimental study on the impact of photodynamic therapy on the tissue structure of normal nasal mucosa in rabbit

    Institute of Scientific and Technical Information of China (English)

    王珊珊; 黄永望; 阴慧娟; 高晓葳; 欧阳杰; 刘学兵

    2014-01-01

    Objective To study the reactive changes of normal nasal mucosa in rabbit under photodynamic therapy (PDT) and to make a preliminary research for the treatment of allergic rhinitis (AR) with PDT.Methods Twenty-four New Zealand rabbits were divided into 2 groups,an experimental group and a control group with 12 rabbits in each group.PDT was applied to the experimental group,while the control group was given no treatment.The nasal mucosa was sampled separately from the same position of the rabbits from the 2 groups on 1st,3rd,7th,14th,21st,28th day.Histomorphological changes of the sampled nasal mucosa were observed under light microscope and transmission electron microscope (TEM).The damage of three tachykinins:substance P (SP),neurokinin A (NKA) and neurokinin B (NKB) of nerve fibers was observed after immunohistochemical staining.Results Compared with the control group,the nasal mucosa tissues from the experimental group had serious inflammatory reaction with basal layer damaged on the 1st and 3rd day after PDT application,the epithelial cells of nasal mucosa were arranged in disorder,and part of cilium shortened and became abnormal or even disappeared,each organelles damaged obviously; on the 7th,14th,21st day,it could be seen that ciliated cell,columnar cell and goblet cell started regeneration,basal cell and lamina propria glands proliferated,and the glands appeared secretion phenomenon; on the 28th day,ciliated columnar epithelium took back the nasal mucosas with small amount of microvilli,and mucous granules were found in the column cells.Nasal immunohistochemical staining of the experimental group from various stages showed that three kinds of neuropeptides were not expressed.Conclusions Normal rabbit nasal mucosas will be temporarily damaged after PDT application,the damaged nasal mucosa begin to recover in one week,and return to normal in about four weeks.Most structure and functions have recovered at the fourth week except some nerve endings.%目的 探

  14. RESEARCHES ON THE PHENOTYPIC CORRELATION BETWEEN DIFFERENT MEASUREMENTS ON THE CARCASS OF RABBITS

    Directory of Open Access Journals (Sweden)

    DANIELA MARCELA TOBĂ (GOINA

    2013-12-01

    Full Text Available The aim of this experiment was to get more experimental data on newmethodologies for rabbit meat production evaluation. The trial was carried out on30 rabbits. The rabbits were weaned at 35 days, having an average live weight of499.11+25.98 g. Rabbits were all slaughtered on the same day, that was on the 84th day of living (at this time the average live weight was 1701.7+34.07, andsome linear measurements (body length, loin width, chest width, carcass lengthchest dept and hind leg length were performed on cold carcasses. The rabbitshave been kept in wire fattening cages: 6 rabbits / cage.

  15. Viral diseases of the rabbit.

    Science.gov (United States)

    Krogstad, Aric P; Simpson, Janet E; Korte, Scott W

    2005-01-01

    Viral disease in the rabbit is encountered infrequently by the clinical practitioner; however, several viral diseases were reported to occur in this species. Viral diseases that are described in the rabbit primarily may affect the integument, gastrointestinal tract or, central nervous system or maybe multi-systemic in nature. Rabbit viral diseases range from oral papillomatosis, with benign clinical signs, to rabbit hemorrhagic disease and myxomatosis, which may result in significant clinical disease and mortality. The wild rabbit may serve as a reservoir for disease transmission for many of these viral agents. In general, treatment of viral disease in the rabbit is supportive in nature. PMID:15585192

  16. Sarcocystis and related organisms in Australian Wildlife: IV. Studies on Sarcocystis cuniculi in European rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Munday, B L; Smith, D D; Frenkel, J K

    1980-04-01

    The role of the cat (Felis domestica) as a definitive host for Sarcocystis cuniculi of European rabbits (Oryctolagus cuniculus) was confirmed. It was shown that after dosing with sporocysts from cats, rabbits developed sarcocysts and these became infective for cats at not less than 93 days post-infection (p.i.). The earliest infection detected was at 142 days p.i. Infected muscle from an experimental rabbit did not transmit Sarcocystis when fed to other rabbits. Microscopically, sarcocysts in European rabbits (O. cuniculus) were morphologically indistinguishable from those in cottontail rabbits (Sylvilagus floridanus). PMID:6776293

  17. Establishment of a cerebral schistosomiasis experimental model in rabbits%家兔脑型血吸虫病动物实验模型的建立及影响因素

    Institute of Scientific and Technical Information of China (English)

    王鹏; 王丹; 陈世洁; 吴明灿; 成祥林; 李军川; 陈廷煊; 朱占胜

    2011-01-01

    in the sham-operation group received saline injection. Rabbits in the normal group received no treatment. Base on the clinical manifestations, rabbits were sacrificed on days 3, 5, 7, 10, 20, and 30 post injection, and brain samples were sectioned and stained with hematoxylin-eosin. Sections were observed under the microscope. Results The rabbits in the operation group exhibited various neurological symptoms, including anorexy, partial and general seizures, and paralysis. The morphological analysis showed several schistosome eggs in the nervous tissue on day 3 post operation, with very mild inflammation. On days 7-10 post operation, several schistosome eggs were localized in proximity to red blood cells with many neutrophilic granulocytes and eosinophilic granulocytes around them. The schistosome eggs developed into the productive granuloma stage on days 14-20 post operation, On day 30, the schistosome eggs were found to be in the healing-by-fibrosis stage, and the granuloma area was replaced by fibrillary glia through astrocytosis. The sham-operation group and the normal group showed negative results. Conclusion This method might be used to establish the cerebral schistosomiasis experimental model. Several factors need to be considered in establishing this model, such as the antigenic property of eggs, the time of scarification, and the clinical manifestations.

  18. Efecto morfológico y funcional vascular de los andrógenos endógenos en un modelo experimental en conejos ateroscleróticos Vascular morphologic and functional effect of endogenous androgens in an experimental atherosclerotic rabbits' model

    Directory of Open Access Journals (Sweden)

    Darío Echeverri

    2007-12-01

    Full Text Available Resumen: estudios clínicos y experimentales previos, sugieren que los andrógenos podrían tener un efecto adverso, neutral o benéfico, sobre la aterosclerosis y sus manifestaciones clínicas. Métodos: se realizó un estudio experimental aleatorizado y controlado en 40 conejos de raza Nueva Zelanda. 20 animales se sometieron a orquidectomía y 20 se alimentaron con dieta aterogénica durante 20 semanas. Se distribuyeron en cuatro grupos: 1: no castrados sometidos a dieta normal; 2: castrados sometidos a dieta normal; 3: no castrados sometidos a dieta aterogénica y 4: castrados sometidos a dieta aterogénica. Se hicieron mediciones de colesterol total y testosterona libre. Después de la eutanasia, se cuantificó en aorta la relajación arterial independiente de endotelio y dependiente de endotelio in-vitro, y se hicieron análisis histomorfométricos de la aorta torácica para cuantificar la formación de placa aterosclerótica. Resultados: los animales sometidos a dieta normal (n=20 tuvieron colesterol total de 51,1 ± 8,5 mg/dL y los sometidos a dieta aterogénica de 429,2 ± 262,0 mg/dL (p Summary: previous clinical and experimental studies suggest that androgens could have an adverse, neutral or beneficial effect on atherosclerosis and its clinical manifestations. Methods: an experimental, randomized controlled study in 40 New Zealand white male rabbits was realized. 20 rabbits underwent orchiectomy and 20 were fed with an atherogenic diet for 20 weeks. These were distributed in four groups: 1. non-castrated under normal diet, 2. castrated under normal diet, 3. non-castrated under atherogenic diet, and 4. castrated under atherogenic diet. Total cholesterol and free testosterone were measured. After euthanasia, arterial relaxation independent of endothelium was quantified in aorta, as well as the one depending on endothelium, in vitro, and histomorphometric analysis of thoracic aorta were made in order to quantify the atherosclerotic

  19. Avaliação do efeito do ibandronato na consolidação de fratura: estudo experimental em coelhos Evaluation of ibandronate effects in bone healing: experimental study in rabbits

    Directory of Open Access Journals (Sweden)

    Roberto Guarniero

    2007-08-01

    Full Text Available OBJETIVO: Determinar a presença de efeito favorável do ibandronato de sódio na consolidação óssea, contribuindo assim para estudos futuros visando sua utilização terapêutica no tratamento de fraturas e pseudartroses. MÉTODOS: Vinte coelhos da raça Nova Zelândia Albino foram submetidos à osteotomia transversa mediodiafisária da fíbula direita e divididos aleatoriamente em dois grupos com 10 animais cada. Os animais do grupo I receberam 2ml de solução contendo ibandronato de sódio e água destilada; os animais do grupo II receberam somente água destilada (grupo controle. Os coelhos foram sacrificados após 30 dias. Os calos ósseos formados no local das osteotomias foram avaliados por densitometria e histomorfometria. Utilizou-se na avaliação estatística dos resultados o teste t para a inferência sobre a diferença das médias de amostras paramétricas e a prova de Mann-Whitney para as amostras não paramétricas. Adotou-se o nível de significância de 5%. RESULTADOS: Observou-se que a quantidade relativa de osso foi maior e a quantidade relativa de fibrose foi menor nos calos ósseos formados no grupo medicado com ibandronato do que no grupo controle. Não houve diferença na quantidade relativa de cartilagem e na densidade mineral dos calos ósseos comparando-se os dois grupos. CONCLUSÃO: Neste experimento a administração do ibandronato de sódio favoreceu a consolidação de osteotomias da fíbula em coelhos, aumentando a quantidade relativa de osso nos calos ósseos formados e diminuindo a quantidade de fibrose.OBJECTIVE: To determine the presence of a favorable sodium ibandronate effect in bone healing, thus contributing for future studies of its clinical use in the treatment of fractures and pseudoarthroses. METHODS: 20 New Zealand white rabbits were submitted to transverse mid-diaphysis osteotomy of the right fibula and divided at random into two groups of 10 animals each. Animals in group I were given 2 ml of a

  20. Absorbable self-reinforced polylactide (SR-PLLA) rods vs rigid rods (K-wire) in spinal fusion: an experimental study in rabbits

    OpenAIRE

    Bezer, Murat; Yildirim, Yakup; Erol, Bülent; Güven, Osman

    2004-01-01

    Several clinical and experimental reports have evaluated the spinal application of bioabsorbable material for plating the anterior lumbar and cervical spine, and in anterior and posterior lumbar interbody spinal fusion. Nevertheless, the use of these materials in posterolateral interlaminar fusion has yet to be elucidated in the literature. The effects of bioabsorbable self-reinforced polylactide rod (SR-PLLA) implantation, rigid fixation (K-wire) and non-implantation with posterior interlami...

  1. Rabbit Model of Retinoblastoma

    OpenAIRE

    Shin Jeong Kang; Grossniklaus, Hans E.

    2011-01-01

    We created a rabbit model of retinoblastoma and confirmed the tumor clinically and histopathologically. Seventeen New Zealand rabbits were immunosuppressed with cyclosporin A at doses of 10–15 mg/kg. At day 3, the animals received a 30 μl subretinal injection of 1 × 1 0 6 cultured WERI retinoblastoma cells. Digital fundus images were captured before euthanasia, and the eyes were submitted for histopathology. Retinoblastoma cells grew in all the inoculated eyes and established a tumor under th...

  2. Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas.

    OpenAIRE

    Spieker-Polet, H; Sethupathi, P; Yam, P C; Knight, K L

    1995-01-01

    During the last 15 years several laboratories have attempted to generate rabbit monoclonal antibodies, mainly because rabbits recognize antigens and epitopes that are not immunogenic in mice or rats, two species from which monoclonal antibodies are usually generated. Monoclonal antibodies from rabbits could not be generated, however, because a plasmacytoma fusion partner was not available. To obtain a rabbit plasmacytoma cell line that could be used as a fusion partner we generated transgenic...

  3. Field and experimental data indicate that the eastern cottontail (Sylvilagus floridanus) is susceptible to infection with European brown hare syndrome (EBHS) virus and not with rabbit haemorrhagic disease (RHD) virus

    OpenAIRE

    Lavazza, A.; P. Cavadini; I. Barbieri; P. Tizzani; A.C. Pinheiro; Abrantes, J.; Esteves, P.J.; G. Grilli; E. Gioia; Zanoni, M.; P. Meneguz; Guitton, J.; S. Merchandeau; M. Chiari; Capucci, L

    2015-01-01

    International audience The eastern cottontail (Sylvilagus floridanus) is an American lagomorph. In 1966, it was introduced to Italy, where it is currently widespread. Its ecological niche is similar to those of native rabbits and hares and increasing overlap in distribution brings these species into ever closer contact. Therefore, cottontails are at risk of infection with the two lagoviruses endemically present in Italy: Rabbit Haemorrhagic Disease virus (RHDV) and European Brown Hare Synd...

  4. Pathogenesis of Herpesvirus sylvilagus infection in cottontail rabbits.

    OpenAIRE

    Hesselton, R. M.; Yang, W.C.; Medveczky, P; Sullivan, J L

    1988-01-01

    Experimental infection with Herpesvirus sylvilagus produces clinical and histopathologic changes in its natural host, the cottontail rabbit (Sylvilagus floridanus), similar to those observed in humans acutely infected with Epstein-Barr virus (EBV). Twenty-seven seronegative cottontail rabbits were infected with Herpesvirus sylvilagus and all developed antibodies within 10 days. Neutralizing antibody was detected as early as 7 days after infection. Virus was isolated from blood mononuclear cel...

  5. Losartan alleviates hyperuricemia-induced atherosclerosis in a rabbit model

    OpenAIRE

    Zheng, Hongchao; Li, Ning; Ding, Yueyou; Miao, Peizhi

    2015-01-01

    Objective: To investigate the mechanisms underlying the therapeutic effects of losartan on hyperuricemia-induced aortic atherosclerosis, in an experimental rabbit model. Methods: Male rabbits (n = 48) were divided into control, hyperuricemia (HU), hypercholesterolemia + hyperuricemia (HC + HU) and high-purine with 30-mg/kg/d losartan (HU + losartan) groups. Serum uric acid (UA) and plasma renin and angiotensin II activities were determined. Aortic tissue specimens were analyzed for histologic...

  6. Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands

    NARCIS (Netherlands)

    Burt, Sara A.; Veltman, Jorg; Hakze-van der Honing, Renate; Schmitt, Heike; Poel, van der Wim H.M.

    2016-01-01

    Rabbits have been suggested as a zoonotic source of Hepatitis E virus. Phylogenetic analysis of HEV isolates from farmed, wild and pet rabbits in the Netherlands (23, 0, and 60 % respectively) showed them to be grouped amongst published rabbit HEV sequences and distinct from most human isolates.

  7. Copy of Acute Renal Failure in Rabbits and Experimental Treatment%急性肾衰竭动物模型的复制及实验性治疗

    Institute of Scientific and Technical Information of China (English)

    葛贺; 李冰; 黄晓莉; 杨丽娟; 陈立松; 芦丽莉; 刘艳波

    2013-01-01

    Objective To copy the acute renal failure ( ARF) animal model and execute an experimental treatment of it in rabbits, to lay a foundation for the implementation of comprehensive experiment for undergraduate students. Method ARF model was set up through the injection of 50% glycerol (10 mL/kg). All the rabbits were monitored with ECG, Sodium bicarbonate solution, furosemide solution and CaCl2 solution were infused through the external jugular vein to the rabbits in the treatment group. Serum urea nitrogen ( BUN), serum creatinine (Cre)level,plasma pH,urine volume and urine creatinine changes of the rabbits were detected after 24 hours. Results The application of 50% glycerol intramuscular injection could replicate typical animal models with acute renal failure. Serum BUN and Cre levels concentration in the model group were obviously higher than those in the negative control group, the urine Cre concentration was also higher, while the urine volume per minute was less and a metabolic acidosis happened at the same time. In the treatment group, serum BUN and Cre levels were significantly lower than those in the model group, while urine volume per minute was more than that in the control group, and the metabolic acidosis was improved. The blood pressure of animals in the treatment group was a litter higher,but there was no difference compared with that in the negative control group, T wave was high and giant, and the QRS wave was widened in the model group compared with those in the negative control group. After the treatment,ECG of the animals in the model group gradually returned to normal state. Conclusion The method of intramuscular injection of 50% glycerol could be reliable to replicate the animal model with acute renal failure, and can be taken as a comprehensive experimental teaching method and widely used in the teaching of medical functional experiment.%目的 复制家免急性肾衰竭动物模型并进行实验性治疗,为本科学生综合性实

  8. Celiac artery in New Zealand rabbit: anatomical study of its origin and arrangement for experimental research and surgical practice Artéria celíaca em coelhos Nova Zelândia: estudo anatômico de sua origem e arranjo para a pesquisa experimental e a prática cirúrgica

    Directory of Open Access Journals (Sweden)

    Marcelo Abidu-Figueiredo

    2008-05-01

    Full Text Available Rabbits have been used as an experimental model in many diseases and for the study of toxicology, pharmacology and surgery in many universities. However, some aspects of their macro anatomy need a more detailed description, especially the abdominal and pelvic arterial vascular system, which has a huge variability in distribution and trajectory. Thirty cadaveric adult New Zealand rabbits, 13 male and 17 female, with an average weight and rostrum-sacral length of 2.5 kg and 40cm, respectively, were used. The thoracic aorta was cannulated and the vascular system was filled with stained latex S-65. The celiac artery and its proximal branches were dissected and lengthened in order to evidence origin and proximal ramifications. The celiac artery emerged between the 12th and 13th thoracic vertebra in 11 (36.7% rabbits; at the level of the 13th thoracic vertebra in 6 (20% rabbits; between the 13th thoracic vertebra and the 1st lumbar vertebra in 12 (40% rabbits; and at the level of the 1st lumbar vertebra in only one (3.3% rabbit. The mean length of the celiac artery was 0.5cm. The celiac artery first branch was the lienal artery, the second branch was the left gastric artery and the hepatic artery arose from the left gastric artery in all the dissected rabbits. No relation was observed between the celiac artery length and the rostrum-sacral length in rabbits. The number of left gastric and lienal artery branches and the distribution of celiac artery origin are not gender dependent.Os coelhos têm sido usados como modelo experimental em diferentes patologias e para estudos de toxicologia, farmacologia e cirurgia em várias universidades. Entretanto apesar de sua grande utilização, muitos aspectos de sua macroanatomia, em especial os que se referem ao sistema vascular arterial que irrigam as viscerais abdomino-pélvicas ainda carecem de uma descrição mais detalhada, pois os vasos arteriais apresentam grande variabilidade na sua distribuição e

  9. Rabbit Repellent Paint

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Five gallons of rabbit repellent paint were sent to George Wilson to be applied on the trees of the Tewaukon tree plot. Mr. Wilson requires a 3 or 4 in. brush for...

  10. The Cutaneous Rabbit Revisited

    Science.gov (United States)

    Flach, Rudiger; Haggard, Patrick

    2006-01-01

    In the cutaneous rabbit effect (CRE), a tactile event (so-called attractee tap) is mislocalized toward an adjacent attractor tap. The effect depends on the time interval between the taps. The authors delivered sequences of taps to the forearm and asked participants to report the location of one of the taps. The authors replicated the original CRE…

  11. Rabbit manure composting

    OpenAIRE

    Llosera Vall, X.; Voltas Aguilar, Jordi; Pujolà Cunill, Montserrat; Soliva Torrentó, Montserrat

    1992-01-01

    Ten samples of rabbit manure representative of the different techniques of treatment and handling used by livestock farms in Catalonia have been characterized. Two of the materials with a different composition have been composted. The origin of materials influence the composting processes and the compost final characteristics. It seems to be relationated with the balance between humification and decomposition processes.

  12. Irreversible electroporation ablation of the hepatic region close to the gallbladder:pathological observation in experimental rabbits%不可逆电穿孔消融兔胆囊侧肝脏病理学观察

    Institute of Scientific and Technical Information of China (English)

    梁冰; 张波; 李书英; 邓春梅; 杨惠霏; 李家亮; 刘建国; 徐克成; 牛立志; 曾健滢; 周亮; 周序珑; 方刚; 姚飞; 邓春娟; 汪媛

    2014-01-01

    目的:研究靠近胆囊0.5 cm处不可逆电穿孔肝脏消融的安全性和有效性。方法采用新西兰白兔3只作为实验动物,在靠近胆囊0.5 cm处肝脏进行不可逆电穿孔消融,并于7 d后观察消融区域的肝脏病理反应和胆囊损伤情况。结果3只动物均成功完成消融。消融7d后,肝脏面原消融区平均直径3.00 cm黄色坏死灶,肝膈面形成1个平均直径1.67 cm类圆形黄色坏死灶。2只实验兔消融部位和腹壁、1只肝叶和膈肌发生小片段浅度粘连。2只肠系膜则与胆囊消融灶区发生严重粘连。光镜下可见肝大片坏死且消失,仅有部分凝固性坏死肝组织残留,外围有少量斑点状钙化,炎性肉芽组织和小胆管显著增生。肝门静脉血管平滑肌损伤不明显,肌层小范围纤维素样变性、胆囊黏膜上皮大部分坏死,胆囊外结缔组织水肿、胆总管部分黏膜上皮坏死,部分上皮仍存在,肌层完好。结论消融部位肝脏组织完全坏死,消融灶和周围组织分界明显,胆囊出现损伤,但无胆囊漏发生。因此,对于靠近胆囊的肝脏肿瘤而言,不可逆电穿孔可能是一种相对安全有效的选择。%Objective To investigate the safety and efficacy of irreversible electroporation (IRE) ablation of hepatic region close (0.5 cm) to the gallbladder. Methods Three New Zealand white rabbits were used for this study. The IRE probes, which were placed in the hepatic tissue, were 0.5 cm apart from the gallbladder, and IRE ablation was performed. Seven days after the treatment the pathological changes of the hepatic ablated zones and gallbladders were examined. Results Successful IRE ablation was accomplished in all 3 rabbits. Seven days after the treatment the experimental rabbits were sacrificed, and their livers and gallbladders were collected and sent for pathological examination. Macroscopically, the hepatic surface of the ablated area showed a yellow

  13. Experimental study of differentiation between tumor and inflammation using MR imaging; Correlation between VX2 tumors and croton oil induced inflammation in the rabbit's femoral muscle

    Energy Technology Data Exchange (ETDEWEB)

    Saginoya, Toshiyuki; Yamaguchi, Keiichiro; Nakano, Masao (Ryukyus Univ., Nishihara, Okinawa (Japan). School of Medicine)

    1994-06-01

    We evaluated the various magnetic resonance (MR) sequences for differentiation between tumors and inflammation. Experimental models of rabbit's tumors (VX2) and inflammation induced by croton oil in the femoral muscle were used for this evaluation. Both types of lesions presented a high intensity area on T2-weighted and heavy T2-weighted images. An inflammatory lesion was revealed with iso-/low intensity area and tumors were defined as high signal lesions on Sinc saturation fat suppression T1-weighted, standard T1-weighted and fat suppression T1-weighted images by the 3 Point Dixon method. T1, T2 values of tumor and inflammatory lesions were also measured. The T1 value of the tumors was 1186[+-]58 msec (n=5) and that of the inflammatory lesion was 1097[+-]40 msec (n=3). The T2 value of the tumors was 71[+-]13 msec (n=5) and that of the inflammatory lesions was 70[+-]5.5 msec (n=3). There were no significant differences between both the T1 and T2 values. Calculated relative proton density was 0.69[+-]0.14 (n=4) in the tumors, and 0.42[+-]0.12 (n=3) in the inflammatory lesions. A statistical difference (p<0.05) was found in both value. These results suggest that a T2-weighted image is not useful for differentiation between tumors and inflammation. And a T1-weighted image might be useful for such differentiation. This phenomenon is related to the difference of the proton density. (author).

  14. The use of Sodium Carboxymethylcellulose to Prevent Intraabdominal Adhesions in Rabbits

    OpenAIRE

    Koç, Yılmaz; ALKAN, Fahrettin; UYAROĞLU, Ayşen

    2003-01-01

    The purpose of the study was to evaluate the effect of a 1% sodium carboxymethylcellulose (SCMC) solution on the prevention of experimentally induced intraabdominal adhesions in rabbits. Thirty rabbits were used and divided into two equal groups. In all rabbits in both groups, ventral midline laparotomies and serosal abrasions on the caecum were performed. In the treatment group, a solution of SCMC and in the control group a NaCI solution were infused into the abdominal cavity before and afte...

  15. Effects of in vitro cultivated Calculus Bovis compound on pulmonary lesions in rabbits with schistosomiasis

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    AIM:To explore the interventional effects and mechanism of in vitro cultivated Calculus Bovis compound preparation(ICCBco) on pulmonary lesions in portal hypertensive rabbits with schistosomiasis. METHODS:The experimental group included 20 portal hypertensive rabbits with schistosomiasis treated by ICCBco.The control group included 20 portal hypertensive rabbits with schistosomiasis treated by praziquantel. The morphological changes of the pulmonary tissues were observed under light and electron microscopy....

  16. Chondromalacia induced by patellar subluxation in the rabbit

    DEFF Research Database (Denmark)

    Møller, B.N.; Møller-Larsen, F.; Frich, Lars Henrik

    1989-01-01

    A unilateral patellar malalignment was induced in 20 young and 20 mature rabbits by lateral displacement of the tibial tuberosity, the other knee serving as osteotomized in situ control. At 6 weeks, all the knees appeared macroscopically normal, but histologically definite cartilage degeneration...... was found on the experimental side. At 3 months, macroscopic changes occurred in 5 of 10 mature rabbits, and histologic cartilage degeneration was found in all the experimental knees, most pronounced in mature animals, and particularly in joint facets submitted to high pressure. This experimental...

  17. Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings Ceratoplastia lamelar experimental em coelhos usando membrana microfibrilar de celulose: achados clínicos, morfológicos e imunoistoquímicos

    Directory of Open Access Journals (Sweden)

    Luciana Riacciardi Macedo

    2010-02-01

    Full Text Available The clinical, histopathological and immunohistochemical features of the cornea were investigated in adult male New Zealand rabbits submitted to lamellar keratoplasty with microfibrillar cellulose membrane. Thirty animals were divided into five groups (n=6 and evaluated up to 60 days after surgery. Clinical examination revealed moderate manifestations of edema, blepharospasm and photophobia on the second day, which became mild or disappeared after the seventh day. This period was characterized clinically by repair of the corneal defect. Histopathological analysis showed the presence of a thin layer of squamous cells covering the damaged area as early as 7th day, accompanied by a mild infiltrate of polymorphonuclear cells. Blood vessels were observed in the epithelium after the 15th day, which had regressed by day 48. Ki67 antibody labeling showed an increase of proliferating cells in the epithelium by the 15th day and in the stroma by day 30. Remodeling and epithelial adhesion were observed during this period. Microfibrillar cellulose membrane (Bionext® used for lamellar keratoplasty was found to yield good results considering the good integration of the implant.Avaliaram-se aspectos clínicos, histopatógicos e imunoistoquímicos da córnes de coelhos da raça Nova Zelândia adultos e machos em ceratoplastias lamelares com membrana de celulose microfibrilar. Trinta animais distribuídos em cinco grupos (n=6 foram estudados por até 60 dias de pós-operatório. A avaliação clínica revelou manifestações moderadas de edema, blefaroespasmo e fotofobia ao segundo dia, evoluindo para formas discretas ou ausentes a partir do sétimo dia, período em que se observou, clinicamente, reparo do defeito corneal. A histopatologia revelou uma fina camada de células escamosas, recobrindo a área lesada já aos sete dias, com discreto infiltrado de células polimorfonucleares. Observaram-se vasos no epitélio a partir do 15o dia, com regressão ao 48o dia

  18. Rabbit care and husbandry.

    Science.gov (United States)

    Bradley, Teresa

    2004-05-01

    This article provides information for the veterinary staff to be better prepared to care for the special needs of rabbit patients as they are presented in increased frequency for veterinary care. Housing, nutrition,restraint, and recognizing illness are covered in detail. Descriptions of techniques for blood collection, oral medication administration, and injection sites are included. Preventive care recommendations for examinations from first visit to geriatric visits are outlined as well as indications for spaying and neutering. Also provided are lists that will aid the veterinary staff in providing instructions when the appointment is made, recommendations for boarding, surgical, and anesthetic considerations and clinical signs that are associated with pain in rabbits. PMID:15145392

  19. Angioarchitecture of rabbit iris.

    OpenAIRE

    Ojima,Makoto; Matsuo,Nobuhiko

    1985-01-01

    We made a scanning electron-microscopic study of the angioarchitecture of the rabbit iris using vascular resin casts, and compared the vascular structure in miosis to that in mydriasis. There were three vascular layers in the iris: the anterior capillary layer, arteriolo-venular layer and posterior capillary layer. The anterior capillary layer was a network which covered the anterior surface of the iris. The posterior capillary layer was a peculiar network composed of many capillary folds, wh...

  20. A experimental study on isolation,culture and identification of osteoblasts from neonatal New Zealand rabbit%新西兰乳兔成骨细胞分离、培养与鉴定的实验研究

    Institute of Scientific and Technical Information of China (English)

    刘小荣; 张笠; 王勇平; 张玉娟; 邹传瑛

    2014-01-01

    Objective To investigate the experimental methods of isolation ,culture and identification of osteoblasts from neo-natal New Zealand rabbits in vitro .Methods Two-step enzymatic digestion was adopted to isolate osteoblasts from skull tissue of neonatal New Zealand rabbits to conduct primary cultured .Inverted phase contrast microscope was employed to study the cellular morphology ,acridine orange fluorescent staining was used to detect the cell adhesion function ,methyl thiazolyl tetrazolium(MTT) assay was employed to measure their proliferation ,and Alizarin red and tetracycline staining were used to test their mineralization . Results Primary osteoblasts were successfully obtained .Inverted phase contrast microscopy showed non-adherent cells were round ,while adherent cells were irregular fusiform ,triangular or polygonal .Acridine orange staining showed the nuclei of osteo-blasts green fluorescence ,with good adhesion ability .Good mineralization ability was also demonstrated by tetracycline and alizarin red staining .Osteoblasts possessed good proliferation activity .Conclusion Utilization of two-step enzymatic digestion contributes to getting a lot of osteoblasts with typical morphological features and biological activity in a short time .%目的:探讨新西兰乳兔成骨细胞体外分离、培养及鉴定的实验方法。方法采用二次酶消化法从新西兰乳兔颅骨组织块分离成骨细胞并进行原代培养,采用倒置相差显微镜进行形态学观察,吖啶橙荧光染色检测其黏附功能,采用四甲基偶氮唑盐(MTT)法检测其增殖情况,采用茜素红及四环素染色检测其矿化功能。结果成功获得原代成骨细胞;倒置相差显微镜显示未贴壁的细胞呈圆形,贴壁生长的细胞呈不规则梭形、三角形或多角形;吖啶橙染色可见成骨细胞的细胞核呈绿色荧光,具有良好的黏附能力;茜素红染色及四环素染色均显示其有良好的钙化能

  1. The researchers developed luminous rabbit

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>Their efforts produced two rabbits out of a litter of eight that went from being a normal, fluffy(蓬松的) white to glowing green in the dark. The rabbits were born at the University of Istanbul as part of a collaboration between scientists from universities in Turkey and Hawaii. The rabbits glow to show that a genetic manipulation technique can work efficiently,

  2. Development of a new experimental model of intramuscular electrical stimulation of the diaphragm in rabbits Desenvolvimento de um novo modelo experimental de estimulação elétrica intramuscular do diafragma em coelhos

    Directory of Open Access Journals (Sweden)

    Rodrigo Guellner Ghedini

    2010-12-01

    Full Text Available PURPOSE: To develop an animal model of diaphragmatic electrical stimulation able to generate an appropriate ventilatory support through the direct implantation of electrodes in the diaphragm (electroventilation. METHODS: Six New Zealand female rabbits (2-3 kg were placed on mechanical ventilation. Then, a laparotomy was performed in order to identify the motor points in each hemidiaphragm, followed by the implantation of the electrodes for diaphragmatic stimulation. We tested two types of electrodes according to the conduction of electrical stimulation: unipolar and bipolar. The electrodes were placed on different occasions in the same animals and tested with current intensities of 20, 26 and 32 mA. Each current intensity was repeated three times for 10 respiratory cycles with 1 minute interval between each cycle, and 5 minutes for new current intensity. We recorded the relationship between current intensity and inspiratory volume. RESULTS: The electrodes adequately stimulate the diaphragm and obtain inspired volumes using different intensity currents. The bipolar electrode generated inspiratory volumes as high as 4.5 times of baseline while the unipolar electrode reached up to 3.5 times of baseline. CONCLUSION: This model has proved to be effective for studying the performance of the diaphragm under different electrical stimulations using different set of electrodes.OBJETIVO: Desenvolver um modelo animal de estimulação elétrica diafragmática capaz de gerar um suporte ventilatório adequado através da implantação direta de eletrodos no diafragma (eletroventilação. MÉTODOS: Seis coelhas da raça Nova Zelândia (2-3 kg foram colocadas em ventilação mecânica. Através de uma laparotomia foram identificados os pontos motores em ambas as cúpulas diafragmáticas, seguido pela implantação dos eletrodos para estimulação diafragmática. Foram testados dois tipos de eletrodos de acordo com a condução do estímulo elétrico: unipolar e

  3. Cistoplastia experimental em coelhos (Oryctolagus cuniculus com peritônio bovino conservado em glicerol a 98% Experimental cystoplasty in rabbits (Oryctolagus cuniculus with bovine peritoneum conserved in 98% glycerol

    Directory of Open Access Journals (Sweden)

    Tatiana Catelan de Oliveira

    2008-11-01

    Full Text Available Neste estudo foi implantado um retalho de membrana peritoneal bovina em substituição a um fragmento da face ventral da bexiga de coelhos albinos, raça Nova Zelândia, adultos, machos não-castrados (n=12, com o intuito de avaliar o processo de reparação tecidual no que se refere à biocompatibilidade, à capacidade de reparação tecidual e a possíveis complicações. Aos sete, 14, 30 e 60 dias de pós-operatório, os animais foram eutanasiados, três em cada período, mediante o emprego de tiopental sódico (200mg kg-1, para posterior avaliação macroscópica e análise histopatológica da interface do implante com o tecido nativo. Macroscopicamente, foram observadas, em todos os períodos, aderências de estruturas adjacentes ao local do implante, presença de cálculos e ausência de sinais de rejeição. Sob microscopia de luz, aos sete, 14 e 30 dias de observação, o implante ainda estava presente, havia intensa reação inflamatória mista, neovascularização, fibroplasia e escassas fibras musculares, contudo, o epitélio e a lâmina própria não estavam reconstituídos. Aos 60 dias de avaliação, o implante não estava mais presente e todas as camadas vesicais encontravam-se reparadas. O implante foi biocompatível e forneceu arcabouço para orientação e desenvolvimento das camadas teciduais da bexiga, mediante processos de reparação, restabelecendo a estrutura do órgão.Bovine peritoneum was utilized to replace a section of ventral face of the bladder of New Zealand rabbits, adult and entire males (n=12 with the purpose of evaluating the tissue repair process in regard of its biocompatility, scar formation ability and possible complications. The animals were euthanized at 7, 14, 30 and 60 days post operative, three in each period, by the use of sodium thiopental (200mg kg-1, followed by macroscopic evaluation and histopathological analysis of the implant interface with the native tissue. Macroscopically it was observed

  4. Adesivo biológico de fibrina na mioescleropexia posterior em coelhos: estudo experimental Fibrin biological adhesive for posterior mioscleral fixation of superior rectus muscles: an experimental study in rabbits

    Directory of Open Access Journals (Sweden)

    Ana Tereza Ramos Moreira

    2004-04-01

    the superior rectus muscle's action of eyes in which the biological adhesive has been used with those used as a control group. METHODS: The sample consisted of 30 animals, 60 eyes. One of the eyes of each animal was treated with biological adhesive (30 eyes, while the other was used as a control (30 eyes. All animals were sacrificed on the 60th postoperative day. Muscle reduction was analyzed and the sizes of the adherences produced at surgery and after 60 days were measured. RESULTS: The fibrin clot was produced immediately after applying the biological adhesive and at the end of few seconds all muscle fibers were adhered to the sclera. All muscles treated with biological adhesive showed reduction in motor function on the 60th postoperative day when compared with the control group. The sizes of the initially produced adherence compared with those after 60 days was reduced 28.48%. There was an ascending and positive but weak correlation (r=0.367204. There must exist other variables affecting adherence reduction. There was no significant inflammatory reaction or any other complication related to the surgery. CONCLUSIONS: The clinical results of motor function demonstrated that the biological adhesive of fibrin produced muscle-scleral adhesion, reducing the arc of contact of the superior rectus muscle in rabbits.

  5. Rabbit lung injury induced by explosive decompression

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective: To study the mechanism of rabbit lunginjury caused by explosive decompression. Methods: A total of 42 rabbits and 10 rats were served as the experimental animals. A slow recompressiondecompression test and an explosive decompression test were applied to the animals, respectively. And the effects of the given tests on the animals were discussed. Results: The slow recompression-decompression did not cause an obvious lung injury, but the explosive decompression did cause lung injuries in different degrees. The greater the decompression range was, the shorter the decompression duration was, and the heavier the lung injuries were. Conclusions: Explosive decompression can cause a similar lung injury as shock wave does. The primary mechanical causes of the lung injury might be a tensile strain or stress in the alveolar wall and the pulmonary surface's impacts on the inside wall of the chest.

  6. Ultrastructure of Reissner's membrane in the rabbit

    DEFF Research Database (Denmark)

    Qvortrup, K.; Rostgaard, Jørgen; Bretlau, P.

    1994-01-01

    Anatomy, Reissner's membrane, electron microscopy, tubulocisternal endoplasmic reticulum, subsurface cisterns, rabbit......Anatomy, Reissner's membrane, electron microscopy, tubulocisternal endoplasmic reticulum, subsurface cisterns, rabbit...

  7. 微量元素锶对实验性兔动脉粥样硬化斑块形成的影响%Effect of Strontium on the development of experimental atherosclerosis in rabbits

    Institute of Scientific and Technical Information of China (English)

    李牧; 杨佳琳; 杨庭树; 郭园园; 李硕磊; 易军

    2012-01-01

    Objective To study the effect of Strontium on the development of experimental atherosclerosis in rabbits. Methods 71 New Zealand white rabbits were randomly assigned to five groups of A ~ E after one week of adaptive feeding: 15 in the pure water control group, 14 in the Strontium-water group of concentration of 3 mg/L, 16 in the Strontium-water group of concentration of 9 mg/L, 12 in the Strontium-water group of concentration of 18 mg/L and 14 in the Strontium-water group of concentration of 36 mg/L. All the rabbits were given high fat diet of 120 g/day for 12 weeks. Serum lipids four total cholesterol (total cholesterol, TC), triglycerides (and triglyceride, TG), high density lipoprotein cholesterol (high-density lipoprotein cholesterol, HDL-C) and low-density lipoprotein cholesterol (low-density lipoprotein cholesterol, LDL-C) were tested at the beginning and end of experiment and serum inflammatory factors including high-sensitivity c-reactive protein (a high sensitive C of reactive protein, hs-CRP), endothelin-1 (of endothelin-1, ET-1), white blood cells interleukin- 2 (interleukin-2, IL-2) and interleukin-6 (interleukin-6, IL-6) were also detected with Elisa. Atherosclerosis area percentage was calculated with image processing software. Aortic HE-stained slices were observed with ordinary microscope and results were categorized to four grades of mild, moderate, severe and no significant changes according to atherosclerosis four classification criteria. Results ①after 12 weeks (on 13th week) of high-fat diet, there was a decreasing trend in the increase in four lipids in all the five groups, and increases in group D and E were much smaller than in group A; ②Hs-CRP, ET-1, IL-2 and IL-6 increased significantly (P 0.05); ③atherosclerotic plaque area gradually reduced (P < 0.05, P < 0.01) with the increase of Strontium-water concentration in the five groups; ④pathological results showed that all five groups of rabbits had the four classes of

  8. Evaluation of recombinant endostatin in the treatment of atherosclerotic plaques and neovascularization in rabbits*

    OpenAIRE

    Mao, Wei; Kong, Jing; Dai, Jin; Huang, Zhao-quan; Wang, Dong-zhi; Ni, Gui-bao; Chen, Min-Li

    2010-01-01

    Objective: Atherosclerotic plaques and neovascularization play an important role in the course of coronary atherosclerosis. This study evaluated the effect of recombinant endostatin on experimental atherosclerotic plaques and neovascularization in rabbits. Methods: Eighteen healthy male rabbits were divided into three groups: control group, atherosclerotic model group, and recombinant endostatin treated group. The atherosclerotic model was established via a high-cholesterol diet after balloon...

  9. Preparation of vulnerable plaques in abdominal aorta: an experimental study in rabbits%家兔腹主动脉易损斑块模型制作的实验研究

    Institute of Scientific and Technical Information of China (English)

    曹海利; 于熙滢; 白彬

    2009-01-01

    目的 建立家兔腹主动脉易损斑块模犁.方法 选取30只雄性纯种新西兰大白兔,随机分为3组:球囊损伤+高脂喂养组、高脂喂养组及普通饲料喂养组.饲养12周后分别给予中国斑点蜂蛇毒和组胺药物触发,诱发斑块破裂及血栓形成.结果 内膜损伤+高脂喂养组存活的8只兔中有10处病变狭窄程度超过50%,并且脂质核心较大、纤维帽厚度小于65 μm,判定以上10处病变为易损斑块.高脂喂养组中的9只仅3处可判定为易损斑块;普通饲料喂养组中未见易损斑块.结论 高脂喂养后行腹主动脉球囊拉伤制作兔易损斑块模型是切实可行的方法.%Objective To establish an animal model of vulnerable plaque in abdominal aorta by pharmacological triggering of atherosclerosis in rabbits. Methods Thirty New Zealand white rabbits were randomly and equally divided into 3 groups: group A, receiving balloon injury together with high lipid diet; group B, receiving high lipid diet only, and group C, receiving regular diet only. After feeding for 12 weeks, all the rabbits in three groups underwent pharmacological triggering with Chinese Russell's viper venom (CRVV) and histamine to induce the rupture of the plaque and the formation of thrombosis. Results In 8 survival rabbits of group A, ten lesions with the stenotic degree over 50% were found, which had large lipid core with the thickness of fibrous cap being thinner than 65μm, by which these ten lesions were judged as vulnerable plaques. In 9 rabbits of group B, only 3 vulnerable plaques could be determined, while no single vulnerable plaque could be identified in the rabbits of group C. Conclusion For establishing an animal model of vulnerable plaque in abdominal aorta in rabbits, balloon injury combined with high lipid diet is a feasible and reliable method.

  10. Hyperlipidemia-associated gene variations and expression patterns revealed by whole-genome and transcriptome sequencing of rabbit models.

    Science.gov (United States)

    Wang, Zhen; Zhang, Jifeng; Li, Hong; Li, Junyi; Niimi, Manabu; Ding, Guohui; Chen, Haifeng; Xu, Jie; Zhang, Hongjiu; Xu, Ze; Dai, Yulin; Gui, Tuantuan; Li, Shengdi; Liu, Zhi; Wu, Sujuan; Cao, Mushui; Zhou, Lu; Lu, Xingyu; Wang, Junxia; Yang, Jing; Fu, Yunhe; Yang, Dongshan; Song, Jun; Zhu, Tianqing; Li, Shen; Ning, Bo; Wang, Ziyun; Koike, Tomonari; Shiomi, Masashi; Liu, Enqi; Chen, Luonan; Fan, Jianglin; Chen, Y Eugene; Li, Yixue

    2016-01-01

    The rabbit (Oryctolagus cuniculus) is an important experimental animal for studying human diseases, such as hypercholesterolemia and atherosclerosis. Despite this, genetic information and RNA expression profiling of laboratory rabbits are lacking. Here, we characterized the whole-genome variants of three breeds of the most popular experimental rabbits, New Zealand White (NZW), Japanese White (JW) and Watanabe heritable hyperlipidemic (WHHL) rabbits. Although the genetic diversity of WHHL rabbits was relatively low, they accumulated a large proportion of high-frequency deleterious mutations due to the small population size. Some of the deleterious mutations were associated with the pathophysiology of WHHL rabbits in addition to the LDLR deficiency. Furthermore, we conducted transcriptome sequencing of different organs of both WHHL and cholesterol-rich diet (Chol)-fed NZW rabbits. We found that gene expression profiles of the two rabbit models were essentially similar in the aorta, even though they exhibited different types of hypercholesterolemia. In contrast, Chol-fed rabbits, but not WHHL rabbits, exhibited pronounced inflammatory responses and abnormal lipid metabolism in the liver. These results provide valuable insights into identifying therapeutic targets of hypercholesterolemia and atherosclerosis with rabbit models. PMID:27245873

  11. 动态增强MRI评价缺血睾丸活力演变的实验研究%Dynamic contrast-enhanced MRI for the detection of testicular ischemia in experimental induced testicular torsion of rabbits

    Institute of Scientific and Technical Information of China (English)

    任彦; 周珉; 周云; 丁粤粤; 方林; 韩啸; 吴继志; 郭万亮; 盛茂; 倪勇彪

    2011-01-01

    Objective To evaluate the dynamic contrast-enhanced MRI (DCE-MRI)in detecting experimentally induced testicular ischemia. Methods Thirty healthy male New Zealand rabbits were randomly assigned into 6 groups. There were 5 rabbits in each of the following experimental groups: ( 1 ) Normal control, (2) Sham-operated, (3) ischemia of 3 h group, (4) ischemia of 6 h group, (5) ischemia of 12 h group, (6) ischemia of 24 h group. In all experiment groups, the right testis served as the internal control while the left testis served as the experimental side. DCE-MRI for each animal lasts about 10 minutes. Signal enhanced ratios (SERs) of ROI for both sides of each group were calculated by a computer, and parameters of SERs of 30 s, 75 s, 120 s and maximal SER were used for statistical analysis.Time intensity curves (TICs) were made for two sides of each group via Excel 2003 software and classified into 4 types. Statistical analysis was performed to compare the differences of SERs between left and right testis by two independent Kolmogorov-Smirnov test. Results In group I and 2, significant enhancement was observed on both testes of 10 rabbits. The enhancement decreased gradually with the elongation of ischemia in torsion groups. Three cases of type Ⅰ and 2 cases of type Ⅱ were observed in group 1,5 cases of type Ⅰ in group two, 2 cases of type Ⅰ and 3 cases of type Ⅱ b in group three, 2 cases of type Ⅰ and 2 cases of type Ⅱ b in group four, 5 cases of type Ⅱ b in group five and 5 cases of type Ⅲ in group six were noticed in the left testes. And in TICs of right testes, all cases showed TICs of type Ⅰ except 2 cases of type Ⅱ a in group six. In four torsion groups, the values for SER75 of the left side were 0. 084%, 0. 076%, 0.164% and 0.065%, while the right side were 0.255%, 0.410%, 0.586% and 0.302% (P <0.05). The values for SER120 in group three, five and six were 0.221% , 0.158% and 0.059% for the left side, and 0.405%,0.522% and 0.207% for the

  12. Proposição de modelo experimental para estudo morfométrico de vasos e células em esclera de coelhos Proposition of an experimental model for the morphometric study of vessels and cells in rabbit sclera

    Directory of Open Access Journals (Sweden)

    Suzana Matayoshi

    2003-08-01

    Full Text Available OBJETIVO: A inexistência de modelos experimentais para estudo de alterações causadas por terapêuticas variadas na esclera motivou os autores à proposição de modelo animal para estudo quantitativo de vasos e células na esclera. MÉTODOS: Por meio de estudo experimental prospectivo, aleatório e mascarado, foram avaliadas 15 coelhas albinas, divididas em 3 grupos. O grupo 1 (controle foi constituído por animais que não foram submetidos a procedimento cirúrgico ou aplicação de medicação; o grupo 2 foi submetido à cirurgia conjuntival e aplicação de esponja embebida em água destilada; e o grupo 3 foi submetido à cirurgia conjuntival e à aplicação de esponja contendo mitomicina C a 0,4 mg/ml. Foi realizada análise histológica morfométrica (contagem de pontos da esclera. RESULTADOS: O grupo 3 mostrou redução dos parâmetros vasculares e aumento dos parâmetros celulares quando comparado aos outros dois grupos. O grupo 2 mostrou diminuição do número de núcleos de células endoteliais e aumento de células inflamatórias comparativamente ao grupo controle. CONCLUSÕES: O modelo apresentado mostrou-se exeqüível para quantificação vascular e celular em esclera de coelhos. O modelo experimental proposto revelou-se de utilidade para quantificação de vasos e células em esclera, viabilizando a detecção de alterações vasculares e celulares esclerais provocadas pela aplicação tópica de mitomicina C a 0,4 mg/ml.PURPOSE: The lack of experimental models to study diverse therapeutical alterations in sclera led the authors to propose an animal model for a quantitative study of scleral vessels and cells. METHODS: This is a randomized, masked, prospective study using 15 female rabbits divided into 3 groups. The first group of animals (control was not submitted to surgery or drug application; the second group was submitted to conjunctival surgery and application of a sponge containing distilled water. The third group was

  13. The Year of the Rabbit

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Every year of the Chinese lunar calendar corresponds with an animal. The rat,ox, tiger, rabbit, dragon, snake, horse,sheep, monkey, rooster, dog and pig make up the Chinese zodiac, which repeats in a12-year cycle. This year’s Chinese NewYear rings in the Year of the Rabbit.

  14. EFEITO DO LEVAMISOL E DO EXTRATO ETANÓLICO DE FOLHAS DE Momordica Charantia SOBRE A DERMATOFITOSE EXPERIMENTAL EM COELHOS EFFECT OF THE LEVAMISOLE AND THE ETHANOLIC EXTRACT OF Momordica charantia LEAVES ON THE EXPERIMENTAL DERMATOPHYTOSIS IN RABBITS

    OpenAIRE

    Olivardo Facó; Cláudio Afonso Pinho Lopes; Ana Karine Rocha de Melo Leite; Viviane Moura Farias; Francisco Martileudo Sousa Silva; Jacy Aurélia Vieira de Sousa; Diana Célia Sousa Nunes-Pinheiro; Luziana Tavares Braga; Cláudio Cabral Campello; José Hélio Costa; Teresa Neuma Albuquerque Gomes Nogueira

    2007-01-01

    O objetivo deste trabalho foi avaliar o efeito do levamisol e do extrato etanólico das folhas de Momordica charantia na dermatofitose experimental. Para tanto, coe¬lhos jovens, Nova Zelândia, machos, divididos em grupos, receberam, por via oral, durante quinze dias consecutivos Tween 20 (1%, controle; n=5), levamisol (25 mg/Kg/PV; n=4) ou extrato etanólico de M. charantia (EE, 10 mg/Kg/PV, n=6) a partir do 15º dia inoculação por Microsporum canis. Foram realizadas as contagens total e diferen...

  15. The White Rabbit project

    CERN Document Server

    Serrano, J; Gousiou, E; van der Bij, E; Wlostowski, T; Daniluk, G; Lipinski, M

    2013-01-01

    White Rabbit (WR) is a multi-laboratory, multi- company collaboration for the development of a new Ethernet-based technology which ensures sub-nanosecond synchronisation and deterministic data transfer. The project uses an open source paradigm for the development of its hardware, gateware and software components. This article provides an introduction to the technical choices and an explanation of the basic principles underlying WR. It then describes some possible applications and the current status of the project. Finally, it provides insight on current developments and future plans.

  16. 99Tc-MDP对兔实验性葡萄膜炎房水及血清细胞因子的影响%99Tc-MDP on aqueous humor and serum cytokines of experimental uveitis in rabbits

    Institute of Scientific and Technical Information of China (English)

    张开颜; 冼文光; 金陈进; 陈海波; 赵普宁

    2009-01-01

    Objective To investigate the therapeutic effects of 99Tc-MDP on the experimental uveitis in rabbits. Methods Twenty-one experimental rabbits were randomly divided into normal sodium control group, dexamethasone administration group and 99Tc-MDP administration group,7 rabbits in each group. ELISA assay was applied to detect the levels of the interleukin-10 (IL-10) and transforming growth factor-β(TGF-β) in the aqueous humor, the levels of tumor necrosis factor-α(TNF-α) and interferon γ (IFN-γ) in serum before establishing the models, at 5 days after establishing the models,at 10 days after drug administration and at 5 days after stopping administration. Results The levels of IL-10 and TGF-β in aqueous humor of normal sodium control group were (86.2±51.2)ng · L-1 and (91.2±67.9)ng · L-1 at 10 days after drug administration lower than (95.1±44.7)ng · L-1 and (135.6±106.3)ng · L-1 at 5 days after establishing the models, respectively. The levels of TNF-α and IFN-γ in serum of normal sodium control group were (151.9±57.9) ng · L-1 and (4.7±1.2)ng · L-1 at 10 days after drug administration lower than (175.6±96.0)ng · L-1 and (5.8±2. 3)ng · L-1 at 5 days after establishing the models,respectively. The levels of IL-10 and TGF-β in aqueous humor of dexamethasone administration group were (106.9±28.5)ng · L-1 and (168.1±256.6)ng · L-1 at 10 days after drug administration higher than (82.7±41.4)ng ·L-1 and (117.4±45.5)ng · L-1 at 5 days after establishing the models,respectively. The levels of TNF-α and IFN-γ in serum of dexamethasone administration group were (80.3±23.4)ng · L-1 and (3.2±2.3)ng · L-1 at 10 days after drug administration lower than (188.2±104.9)ng · L-1 and (5.7±3.6)ng · L-1 at 5 days after establishing the models,respectively. The levels of IL-10 and TGF-β in aqueous humor of 99Tc-MDP administration group were (136.2±47.2) ng · L-1 and (183.1±79.6) ng · L-1 at 10 days after drug administration higher than (98

  17. Efeito da utilização de um centrifugado de medula óssea no tratamento de lesão muscular: estudo experimental em coelhos Effect of use of bone-marrow centrifugate on muscle injury treatment: experimental study on rabbits

    Directory of Open Access Journals (Sweden)

    Daniel Ferreira Fernandes Vieira

    2011-01-01

    Full Text Available OBJETIVO: O objetivo deste estudo foi de avaliar o efeito de um centrifugado de medula óssea na cicatrização de lesão muscular em coelhos. MÉTODOS: Este estudo experimental envolveu a utilização de 15 coelhos machos, adultos, da raça Nova Zelândia Branco. Em cada animal, realizou-se uma lesão transversal na região média do músculo tibial anterior direito, onde se adicionou uma esponja de colágeno absorvível, embebida em um centrifugado de aspirado de medula óssea do osso ilíaco ipsilateral. O membro posterior esquerdo foi utilizado como controle, sendo submetido à mesma lesão; porém, neste caso, adicionando-se somente a esponja de colágeno absorvível. Após 30 dias, os animais foram sacrificados para o estudo da cicatrização muscular. As áreas de cicatrização muscular foram submetidas à análise histológica com histomorfometria, interessando-se a mensuração do número de células musculares em regeneração por micrômetro quadrado e a proporção de fibrose formada. RESULTADOS: O método de centrifugação utilizado neste estudo resultou em uma concentração média de células nucleadas maior que o número destas células nos aspirados originais, sem provocar destruição celular significativa. A adição do centrifugado de medula óssea não resultou em aumento significante do número de células musculares em regeneração em relação ao grupo controle. Também não houve diferença significante na proporção de fibrose formada em relação ao grupo controle. CONCLUSÃO: A administração de centrifugado de medula óssea utilizada neste estudo não favoreceu a cicatrização de lesão muscular em coelhos.OBJECTIVE: The objective of this study was to evaluate the effect of bone-marrow centrifugate on the healing of muscle injuries in rabbits. METHODS: This experimental study involved use of fifteen adult male New Zealand White rabbits. Each animal received a transverse lesion in the middle of the right

  18. INFLUENCE OF NATURAL IMMUNOMODULATORS ON PROTEIN FRACTIONS AND CORTISOL CONTENT IN RABBIT BLOOD UNDER STRESS

    Directory of Open Access Journals (Sweden)

    Grabovskyi S.

    2015-08-01

    feed increases the Prealbumin concentration in rabbits blood plasma of two experimental groups twice (P ≤ 0.05 and 2.8 times (P ≤ 0.01 compared to the control. Cortisol level in rabbits (which further added to the feed of spleen extract (I research group blood plasma was reliable lower by 40 % (Р<0.05 compared to control, that may to indicate decreasing of stress before slaughter. On the rabbits feeding final stage is necessary to consider a pre-slaughter stress and to apply of natural origin biologically active substances. The results which obtained can to use in researches of stress hormones, such as cortisol, on farm animals for organism resistance increasing, correction and avoid their pre-slaughter stress and improve product quality.

  19. Intrinsic connectivity of neural networks in the awake rabbit.

    Science.gov (United States)

    Schroeder, Matthew P; Weiss, Craig; Procissi, Daniel; Disterhoft, John F; Wang, Lei

    2016-04-01

    The way in which the brain is functionally connected into different networks has emerged as an important research topic in order to understand normal neural processing and signaling. Since some experimental manipulations are difficult or unethical to perform in humans, animal models are better suited to investigate this topic. Rabbits are a species that can undergo MRI scanning in an awake and conscious state with minimal preparation and habituation. In this study, we characterized the intrinsic functional networks of the resting New Zealand White rabbit brain using BOLD fMRI data. Group independent component analysis revealed seven networks similar to those previously found in humans, non-human primates and/or rodents including the hippocampus, default mode, cerebellum, thalamus, and visual, somatosensory, and parietal cortices. For the first time, the intrinsic functional networks of the resting rabbit brain have been elucidated demonstrating the rabbit's applicability as a translational animal model. Without the confounding effects of anesthetics or sedatives, future experiments may employ rabbits to understand changes in neural connectivity and brain functioning as a result of experimental manipulation (e.g., temporary or permanent network disruption, learning-related changes, and drug administration). PMID:26774609

  20. A second rabbit kappa isotype

    OpenAIRE

    1982-01-01

    Immunoglobulin G (IgG) from the rabbit strain Basilea was previously shown to contain two distinct populations of molecules one with light chain belonging to the known lambda isotype and the others to a new kappa-like L chain type. Alloantisera prepared against the Basilea IgG are directed against the kappa-like light chain (anti-bas antisera). All Basilea rabbits express kappa-like chains recognized by anti-bas sera, but IgG from other domestic rabbits did not react with these antisera. Gene...

  1. Experimental Study of Yishenhuoxue Decoction for Treating Knee Osteoarthritis of the Rabbit%益肾活血方治疗兔膝骨性关节炎的实验研究

    Institute of Scientific and Technical Information of China (English)

    李晓萍; 王玉红; 李学胜

    2011-01-01

    Objective:To study the therapeutic effect and possible mechanisms of Yiqihuoxue decoction on knee osteoarthritis models of rabbit. Methods:Thirty New Zealand rabbits were randomly divided into normal control group, model group, and treatment group. All animals in model group and treatment group were managed by immobilization method. One week later,the rabbits in normal control group and model group were treated with physiological saline, while, the rabbits in treatment group were treated with Yiqihuoxue decoction. The histopathology,level of IL-1,TNF-α,and MMP-3 of synovial fluid were observed six weeks after intragastric administration. Results: In histopathology observation, the cartilage damage was reduced in the drug treatment group after 6 weeks. The level of IL-1 、TNF-α,and MMP-3 of synovial fluid in the treatment group were obviously lower than the model group (P<0.05). Conclusion: Yiqihuoxue decoction can reduce cartilage damage in rabbit model of knee osteoarthritis. And it can inhibit the releasing of inflammatory cytokines (IL-l, TNF-α, etc) and MMP-3 effectively, thereby delaying the degradation of the cartilage.%目的:观察益肾活血方对家兔膝骨性关节炎模型的疗效及作用机制.方法:将30只新西兰大耳兔随机分成3组,用制动法复制膝骨性关节炎模型.造模1周后,正常组和模型组用生理盐水,药物组用益肾活血方灌胃.于灌胃第6周后,对组织病理学,关节液中IL-1、TNF-α和MMP-3含量的影响进行观察.结果:病理组织学观察药物组软骨破坏减轻,与模型组比较,关节液中IL-1、TNF-α和MMP-3含量明显降低(P<0.05).结论:益肾活血方能缓解兔膝骨性关节炎模型关节软骨破坏,并能有效抑制炎症细胞因子(IL-1,TNF-α等)、MMP-3的释放,从而延缓对软骨的降解作用可能是其作用机制.

  2. Amiodarone Induced Morphological Changes in Rabbit Pneumocytes

    Directory of Open Access Journals (Sweden)

    Fereshteh Mehraein

    2009-01-01

    Full Text Available Objective: Amiodarone as an iodinated benzofuran derivative is a potent antiarrhythmicagent currently used for the treatment of ventricular arrhythmias. Pulmonary toxicityis one of the complications of Amiodarone therapy. The aim of this study was todetermine the toxicity of Amiodarone for pneumocytes.Materials and Methods: 14 male white New Zealand rabbits were divided in a controlgroup and an experimental group. The experimental group was subjected to intraperitoneal injection with a single daily dose of 80 mg/kg Amiodarone for two weeks.The control group received only normal saline. At the end of the injection period, thetwo groups were anesthetized and perfused with Karnovsky fixative. The lung tissuewas removed and fixed, then prepared for light and electron microscope studies.Morphometric studies were made on sections to find nucleus profile dimensions.Results: Light microscope observation showed acute changes in the alveolus includingcongestion of alveolar capillaries and infiltration of red blood cells (RBCs intothe lumen of the alveoli. Electron microscope study of lung tissue revealed abnormalinclusion bodies within type ΙΙ & Ι pneumocytes. The micrographs also showedthe presence of vacuoles in 5% of the type ΙΙ pneumocytes. Morphometric studiesshowed that the nucleus of the cells in the experimental group were smaller than inthe control group (p<0.01.Conclusion: These results indicate that Amiodarone administration can cause damageto pnuemocytes and the alveolus of rabbit lung, so the effectiveness of Amiodaronein long term treatment of heart failure patients is limited because of the developmentof lung toxicity.

  3. Effects of experimental infections with larvae of Eustrongylides ignotus Jäegerskiold, 1909 and Contracaecum multipapillatum (Drasche, 1882 Baylis, 1920 in rabbits Efeitos de infecções experimentais em coelhos com larvas de Eustrongylides ignotus Jäegerskiold, 1909 e Contracaecum multipapillatum (Drasche, 1882 Baylis, 1920

    Directory of Open Access Journals (Sweden)

    L.A. Barros

    2004-06-01

    Full Text Available Rabbits were infected per os with 10 Eustrongylides ignotus L4 and with 50 Contracaecum multipapillatum L3 per rabbit, recovered from naturally infected freshwater fishes (Hoplias malabaricus in order to evaluate the patogenicity of these two nematode species in mammalian host. Two rabbits (20% infected with E. ignotus died before the fourth day post-inoculation (one after 51 and the other after 78 hours. Six rabbits (60% were inappetent until the fifth day following experimental inoculation. No clinical signs in rabbits inoculated with C. multipapillatum were observed; nevertheless, eight (80% animals were positive for this nematode species. Rabbits inoculated with E. ignotus, had gastric congestion with hematoma of the gastric wall in 60% of the cases. Peritoneum was congested in 20% of the animals with the presence of peritoneal abscess in 10% of the cases. All inoculated animals showed hyperemia of the gastric mucosa with hemorrhagic gastritis due to infections with E. ignotus. In C. multipapillatum inoculated animals, the hyperemia was followed by disruption of the epithelial mucosa in the sites of parasite attachment. In the gastric mucosa, miscellaneous leukocitary infiltrates, with multifocal necrosis reaching the submucosa in the infections with C. multipapillatum were observed under bright field microscopy. Perforating lesions in several organs, mainly in the gastric wall, pancreas and liver, always in the presence of a mixed inflammatory process, intensely fibrous, with hemorrhage and necrosis were observed in animals infected with E. ignotus.Coelhos foram infectados experimentalmente per os com 10 larvas L4 de Eustrogylides ignotus (n= 10 e 50 L3 de Contracaecum multipapillatum (n= 50 coletados em traíras (Hoplias malabaricus naturalmente parasitadas a fim de se avaliar a patogenicidade induzida por essas espécies de nematóides em mamíferos. Dois coelhos (20% infectados com E. ignotus morreram antes do quarto dia p

  4. Rabbit model of rotavirus infection.

    OpenAIRE

    Conner, M E; Estes, M K; Graham, D Y

    1988-01-01

    A new small animal model was developed to study parameters of rotavirus infections, including the active immune response. Seronegative New Zealand White rabbits (neonatal to 4 months old) were inoculated orally with cultivatable rabbit rotavirus strains Ala, C11, and R2 and with the heterologous simian strain SA11. The course of infection was evaluated by clinical findings, virus isolation (plaque assay and enzyme-linked immunosorbent assay), and serologic response. All four strains of virus ...

  5. Ultrasonographic Characteristics of Rabbit's Pancreas

    OpenAIRE

    DIMITROV, Rosen; RUSSENOV, Anton; STAMATOVA-YOVCHEVA, Kamelia; UZUNOVA, Krassimira; Yordanova, Violeta

    2013-01-01

    Aim of the study was to demonstrate some ultrasonography specifications of the normal pancreas in rabbit and their use as model for visual anatomical imaging study of pancreatic lesions in animals and humans. We used 12 clinically healthy 8 months old of New Zealand White rabbits between 2.8 and 3.2 kilos, who were mature and all anesthetized. Our investigation had been done Diagnostic Ultrasound System and micro convex multi frequency transducer. The trial animals were starved before the exp...

  6. Postural performance in decerebrated rabbit

    OpenAIRE

    Musienko, P. E.; Zelenin, P. V.; Lyalka, V. F.; Orlovsky, G. N.; Deliagina, T. G.

    2008-01-01

    It is known that animals decerebrated at the premammillary level are capable of standing and walking without losing balance, in contrast to postmammillary ones which do not exhibit such behavior. The main goals of the present study were, first, to characterize the postural performance in premammillary rabbits, and, second, to activate the postural system in postmammillary ones by brainstem stimulation. For evaluation of postural capacity of decerebrated rabbits, motor and EMG responses to lat...

  7. Comparative study of bridge plate associated to the intramedullary pin and the dynamic compression plate on the experimental osteotomy fixation of femoral in rabbits (Oryctolagus cuniculus). Clinical, radiographic, histological and scintigraphy analyses; Estudo comparativo da placa em ponte associada ao pino intramedular e da placa de compressao dinamica na fixacao de osteotomia experimental em femur de coelhos (Oryctolagus cuniculus). Analises clinica, radiografica, histologica e cintilografica

    Energy Technology Data Exchange (ETDEWEB)

    Borges, Natalie Ferreira

    2011-07-01

    The objectives were to benchmark and monitor the fracture healing of femoral osteotomy in rabbits after fixation with dynamic compression plate and bridge plate associated with intramedullary pin. Were used 14 New Zealand rabbits, four months old with mean weight of 3.5 pounds, from the Experimental Farm Professor Helio Barbosa of the Veterinary School of Minas Gerais Federal Univ. (UFMG). The animals were randomly divided into two groups (I and II). All rabbits underwent osteotomy across the middle third of right femur. In the group I was made to fix the osteotomy with the bridge plate (BP) associated with intramedullary pin, introduced the technique of minimal invasion. In group II, we used dynamic compression plate (DCP) via the conventional approach. Both groups were evaluated clinical, radiographic, histologic and scintigraphic findings. Clinical assessments were performed weekly until the 12{sup th} postoperative week and radiographic examinations were performed before, immediately after, at 15, 30, 45, 60 and 90 days. The bone scintigraphy were performed before and at 20, 50 and 90 days after surgery to monitor the bone metabolism qualitatively and quantitatively. Observed perfusion and bone healing process. After 90 days of study, there was histopathologic evaluation of the osteotomized area and the insertion of screws. In the region of the osteotomy was observed predominance of trabecular bone in group I and group II, the predominance of bone osteons, compatible with the original bone. On insertion of the screws did not differ between groups and there was cortical discontinuity, little necrosis and local hemorrhage. The two types of fixation have led to consolidation within the scheduled period, maturing in early fixation with DCP fixation compared with BP. Scintigraphy demonstrated by the indices of activity and image characteristics, the process of bone healing was significantly greater in animals undergoing early fixation with DCP. (author)

  8. Induction of Asherman's Syndrome in Rabbit

    Science.gov (United States)

    Bazoobandi, Sanaz; Tanideh, Nader; Rahmanifar, Farhad; Tamadon, Amin; Keshtkar, Mohammadreza; Mehrabani, Davood; Kasraeian, Maryam; Koohi-Hosseinabadi, Omid

    2016-01-01

    Background: Uterine synechiae or Asherman's syndrome is a condition that can cause infertility. The present experimental study was designed to establish the rabbit as an animal model for human Asherman's syndrome using the endometrial curettage. Methods: In an experimental study, female adult rabbits (n=18) were randomly divided into intact and ovariectomized groups. One third of caudal part of both uteri was submitted to traumatic endometrial curettage. One group was simultaneously ovariectomized. The intact rabbits were artificially induced ovulation during 10 days after surgery. One third of cranial part of both uteri was selected as the control. Synechiae occurring, luminal area/total area (LA/TA), endometrial area/total area (EA/TA), myometrial and perimetrial area/total area (MPA/TA), endometrial area/uterine wall area (EA/UWA), and myometrial and perimetrial area/uterine wall area (MPA/UWA) ratios of both uteri in six subdivided groups (n=6) were analysed in curetted and intact control parts. On days 15, 30 and 45 following surgery by two-way ANOVA and LSD test (p<0.05). Results: Histopathologic findings showed significant epithelial damage together with significant inflammatory reaction in the intact curettage group. The LA/TA ratios of the intact curettage group on days 15 and 45 were more than the intact control group on day 15. The EA/TA ratio of the intact curettage group on day 30 was less than the intact control group on day 30. Conclusion: Uterine fibrosis was observed in intact curettage group, and this modified animal model showed a pathogenesis condition similar to intrauterine adhesions observed in human. PMID:26962478

  9. Dissociative anesthetic combination reduces intraocular pressure (IOP in rabbits

    Directory of Open Access Journals (Sweden)

    Ewaldo de Mattos-Junior

    2014-02-01

    Full Text Available The aim of this study was evaluate the effects of three anesthetic combinations, ketamine-midazolam, ketamine-xylazine and tiletamine-zolazepam, on IOP in rabbits. In a experimental, blind, randomized, crossover study, six rabbits were anesthetized with each of 3 treatments in random order. Groups KM (ketamine, 30 mg/kg + midazolam, 1 mg/kg; KX (ketamine, 30 mg/kg + xylazine, 3 mg/kg; and TZ (tiletamine + zolazepam, 20 mg/kg. The drugs were mixed in the same syringe injected intramuscularly (IM into the quadriceps muscle. IOP was measured before drug administration (baseline and at 5-minute intervals for 30 minutes. The data were analyzed by a 2-way repeated measures ANOVA followed by Bonferroni test. All groups had significant decreases in IOP compared to baseline (p 0.05. Administration of either ketamine-midazolam, ketamine-xylazine, or tiletamine-zolazepam similarly decrease IOP in rabbits within 30 minutes of injection.

  10. 睾酮对雄兔膝骨关节炎预防作用的实验研究%Experimental study on preventive effects of testosterone on knee osteoarthritis in male rabbits

    Institute of Scientific and Technical Information of China (English)

    熊华章; 刘毅; 吴术红; 邹刚

    2012-01-01

    Objective:To investigate the preventive effect of testosterone on knee osteoarthritis in male rabbits. Methods: Eighteen male rabbits were randomly divided into castration group, noncastration group and testosterone supply group (n=6). The rabbits in castration group underwent the excision of anterior cruciate ligment and the medial meniscus on the right knee joint while those in non-castration group and testosterone supply group underwent the excision of bilateral testicles along with the treatment in castration group. Testosterone undecanoate(6 mg/kg,once every two weeks,three times in total) was injected intramuscularly to rabbits in testosterone supply group at the third week after the operation. All rabbits were killed by gas embolism at the eighth week. Macroscopic evaluation was performed on the articular cartilage of medial malleolus of femur. Insulin-like growth factors (IGF-1 ) immunohistochemical examination was performed in order to investigate the expression of IGF-1 in articular cartilage and the improved Mankin's scores was applied to test the serum levels of total testosterone at different stages. Results: Expressions of IGF-1 were higher in noncastration group and testosterone supply group than in castration group(P<0.05). Mankin's scores were lower in noncastration group and testosterone supply group than in castration group(P<0.05). The serum level of testosterone was lower in castration group than in noncastration group and testosterone supply group at the fourth and eighth week(P<0.05). Conclusion: Testosterone has preventive effect on knee osteoarthritis in male rabbits.%目的:探讨睾酮对雄兔膝骨关节炎( Osteoarthritis,OA)预防的作用.方法:将18只雄性家兔随机分为去势组、未去势组和睾酮补充组,每组6只,未去势组切除右膝关节前交叉韧带和内侧半月板,去势组和睾酮补充组在切除右膝关节前交叉韧带和内侧半月板同时切除双侧睾丸,睾酮补充组于术后第3

  11. Study of hypoglycemic activity of Tinospora cordifolia in alloxan induced diabetic rabbits

    Directory of Open Access Journals (Sweden)

    Saleem B. Tamboli

    2013-10-01

    Full Text Available Background: This study was designed to investigate the hypoglycemic effect of Tinospora Cordifolia in alloxan-induced diabetic rabbits. Methods: Rabbits were divided into four experimental groups: Non-diabetic rabbits treated with normal saline, Non-diabetic rabbits treated with TCREt, Diabetic rabbits treated with Metformin, Diabetic rabbits treated with TCREt. Treatment with drugs was started on the 8th day of alloxan treatment (i.e. day 1 and was continued for 30 days. Statistical evaluation was done using student’s ‘t’ test & one way ANOVA followed by Tuckey`s post hoc test. ‘p’ value less than 0.05 was considered statistically significant. Results: TCREt showed hypoglycemic action in alloxan induced diabetes rabbits. It did not produce hypoglycemia in non diabetic rabbits. Conclusion: Hypoglycemic action of TCREt is comparable to that of standard oral hypoglycemic drug metformin. [Int J Basic Clin Pharmacol 2013; 2(5.000: 559-561

  12. The White Rabbit Project

    CERN Document Server

    Serrano, J; Cattin, M; Garcia Cota, E; Lewis, J; Moreira, P; Wlostowski, T; Gaderer, G; Loschmidt, P; Dedic, J; Bär, R; Fleck, T; Kreider, M; Prados, C; Rauch, S

    2009-01-01

    Reliable, fast and deterministic transmission of control information in a network is a need formany distributed systems. One example is timing systems, where a reference frequency is used to accurately schedule time-critical messages. TheWhite Rabbit (WR) project is a multi-laboratory and multi-company effort to bring together the best of the data transfer and timing worlds in a completely open design. It takes advantage of the latest developments for improving timing over Ethernet, such as IEEE 1588 (Precision Time Protocol) and Synchronous Ethernet. The presented approach aims for a general purpose, fieldbus-like transmission system, which provides deterministic data and timing (sub-ns accuracy and ps jitter) to around 1000 stations. It automatically compensates for fiber lengths in the order of 10 km. This paper describes the WR design goals and the specification used for the project. It goes on to describe the central component of the WR system structure - the WR switch - with theoretical considerations a...

  13. Adjunctive hyperbaric oxygen therapy in the treatment of atrophic tibial nonunion with Ilizarov external fixator: a radiographic and scintigraphic study in rabbits*

    OpenAIRE

    Kurklu, Mustafa; Yurttas, Yuksel; Kose, Ozkan; Demiralp, Bahtiyar; Yuksel, Halil Yalcin; Komurcu, Mahmut

    2012-01-01

    Objective: The aim of this experimental study was to determine the effects of adjunctive hyperbaric oxygen therapy (HBO) on atrophic tibial nonunion treatment using Ilizarov external fixator. Methods: Twenty New Zealand white rabbits were randomly divided into two equal groups. A circular external fixator was applied to the right tibia of all the rabbits. A 5-mm bone block was resected and a tibial pseudarthrosis was obtained after a 6-month waiting period. The experimental group rabbits ...

  14. Inhibitory effect of syphilitic rabbit serum on DNA synthesis in rabbit cells in vitro.

    OpenAIRE

    Wong, G H; Steiner, B; Strugnell, R; Faine, S.; Graves, S.

    1984-01-01

    A previously described toxic factor associated with Treponema pallidum (Nichols) and found in extracts of syphilitic rabbit testes has now also been detected in syphilitic rabbit serum. The toxic factor, which inhibits DNA synthesis in baby rabbit genital organ (BRGO) cells in vitro, is present in rabbit serum up to 30 days after infection with T pallidum.

  15. Pathogenesis of Herpesvirus sylvilagus infection in cottontail rabbits.

    Science.gov (United States)

    Hesselton, R M; Yang, W C; Medveczky, P; Sullivan, J L

    1988-12-01

    Experimental infection with Herpesvirus sylvilagus produces clinical and histopathologic changes in its natural host, the cottontail rabbit (Sylvilagus floridanus), similar to those observed in humans acutely infected with Epstein-Barr virus (EBV). Twenty-seven seronegative cottontail rabbits were infected with Herpesvirus sylvilagus and all developed antibodies within 10 days. Neutralizing antibody was detected as early as 7 days after infection. Virus was isolated from blood mononuclear cells, spleen, bone marrow, thymus, lymph nodes, kidneys, lung, and liver as early as 3 days after infection. Infected animals showed leucocytosis, monocytosis, and lymphocytosis with the appearance of atypical lymphocytes. Peripheral blood abnormalities peaked at 10-14 days after infection, and returned to normal by 28 days after infection, with the exception of atypical lymphocytosis that persisted in some animals for more than 2 years after experimental infection. More severe histopathologic changes were seen in virus-infected juvenile rabbits than adult rabbits; these changes included viral myocarditis, interstitial pneumonia, and lymphocytic myositis. Reactive hyperplasia and subsequent lymphocytic depletion of spleen and lymph nodes were reminiscent of that seen in virus-associated hemophagocytosis syndrome. Prominent lymphoid hyperplasia of many nonlymphoid organs, most notably the kidney and lungs, was observed. The development of these lymphoproliferative lesions and other lymphoid changes during H. sylvilagus infection suggest that this system may be a model to study similar lesions induced by EBV infection in humans. PMID:2849303

  16. 兔VX2脑瘤血管生成的灌注CT研究%Experimental study on angiogenesis in rabbit VX2 brain tumor using perfusion CT

    Institute of Scientific and Technical Information of China (English)

    康立清; 张云亭; 孙世梅

    2006-01-01

    Objective: To study the perfusion CT features of rabbit VX2 brain tumor with correlation to MVD and VEGF, and to validate perfusion CT for reflection of tumor angiogenesis. Methods: Rabbit VX2 brain tumor model was established by injection of 100 μL viable tumor cells (107/mL) through a 2 mm-hole 5 mm to the right of the sagittal suture and 5 mm posterior to the coronal suture bored by dental drill. MRI was performed every 2 days after seven days of implantation to evaluate the growth of the tumor. Twenty New Zealand White rabbits with tumor size over 3 mm in diameter were randomly divided into 2 groups according to the tumor growth time with those less than 3 weeks as group 1 and those more than 3 weeks as group 2, and perfusion CT were performed accordingly. CT measurements of BV, BF and PS from tumor, peritumor and contralateral normal tissue regions were obtained. After that the animals were sacrificed and 2% Evans blue (2 mL/kg) was given intravenously in 16 of these animals 1 h prior to sacrifice to detect breakdown of the blood brain barrier. VEGF and MVD were evaluated in immunohistochemical examination of the specimens. Results: Tumor had significantly higher BV, BF and PS (P=0.000) than peritumor and normal tissue region. Tumor BV, BF and MVD in group 2 were significantly higher than that in group 1 (P<0.01).Significant linear correlation was found between MVD and BV (t=0.915, P=0.000), MVD and BF (r=0.901, P=0.000), and MVD and PS (r=0.459, P=0.042). We also found a rank correlation between PS and blue stain of tumor (rs=0.861, P=0.000). Conclusion: Perfusion CT can distinguish tumor from peritumor and normal tissue clearly, reflect tumor angiogenesis accurately, and provide useful information for the evaluation of brain tumor.

  17. Differential Fault Analysis of Rabbit

    Science.gov (United States)

    Kircanski, Aleksandar; Youssef, Amr M.

    Rabbit is a high speed scalable stream cipher with 128-bit key and a 64-bit initialization vector. It has passed all three stages of the ECRYPT stream cipher project and is a member of eSTREAM software portfolio. In this paper, we present a practical fault analysis attack on Rabbit. The fault model in which we analyze the cipher is the one in which the attacker is assumed to be able to fault a random bit of the internal state of the cipher but cannot control the exact location of injected faults. Our attack requires around 128 - 256 faults, precomputed table of size 241.6 bytes and recovers the complete internal state of Rabbit in about 238 steps.

  18. A single immunization with a dry powder anthrax vaccine protects rabbits against lethal aerosol challenge

    OpenAIRE

    Klas, S.D.; Petrie, C.R.; Warwood, S.J.; Williams, M S; Olds, C.L.; Stenz, J.P.; Cheff, A.M.; Hinchcliffe, M.; Richardson, C.; Wimer, S.

    2008-01-01

    Here we confirm that intranasal (IN) dry powder anthrax vaccine formulations are able to protect rabbits against aerosol challenge 9 weeks after a single immunization. The optimum dose of rPA in our dry powder anthrax vaccine formulation in rabbits was experimentally determined to be 150 μg and therefore was chosen as the target dose for all subsequent experiments. Rabbits received a single dose of either 150 μg rPA, 150 μg rPA + 150 μg of a conjugated 10-mer peptide representing the B. anthr...

  19. Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review.

    OpenAIRE

    Abrantes Joana; van der Loo Wessel; Le Pendu Jacques; Esteves Pedro J

    2012-01-01

    Abstract Rabbit haemorrhagic disease virus (RHDV) is a calicivirus of the genus Lagovirus that causes rabbit haemorrhagic disease (RHD) in adult European rabbits (Oryctolagus cuniculus). First described in China in 1984, the virus rapidly spread worldwide and is nowadays considered as endemic in several countries. In Australia and New Zealand where rabbits are pests, RHDV was purposely introduced for rabbit biocontrol. Factors that may have precipitated RHD emergence remain unclear, but non-p...

  20. Effect of transcutaneous electric stimulation on the cardiac electrical activity in New Zealand white rabbits

    Directory of Open Access Journals (Sweden)

    Wang ZHANG

    2015-10-01

    Full Text Available Objective To study the effect of transcutaneous electric stimulation on the cardiac electrical activity in New Zealand white rabbits, in order to search a safety threshold for clinical electrical stimulation therapy, as to provide the theoretical basis for the design of in vitro pacemaker. Methods New Zealand white rabbits were randomly assigned into 17 groups (6 each. Rabbits in 16 experimental groups were given 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75 and 80V electrical stimulation, respectively, with the stimulating site designated at epigastric region. BL -420F biological function experimental system was employed to supply the power and acquire the ECG, with the output pulse electrical stimulation frequency set at 270 times/minute, and the stimulating wave as square wave. A control group was set, in which the stimulating voltage was set to 35V, the stimulant anode was located in the anterior chest area, and the cathode was on the skin surface of back corresponding to the site of the heart, and the rest was the same as in experimental groups. Results No stimulation rhythm was observed in rabbits of those experimental groups with voltage ≤35V, but all stimulation rhythm was observed in rabbits of control group. No arrhythmia occurred in rabbits of those experimental groups with voltage ≤30V, while the heart rate was slowed down after stimulation in rabbits of the experimental groups with voltage ≥45V stimulation. In rabbits receiving stimulation with voltage ≤35V there was no dystropy or light dystropy, but with no visible injury to the local tissues. No visible injury was observed in the rabbits undergoing stimulation with voltage ≤40V. Conclusion Pulse electric stimulation with voltage ≤35V in the epigastric region would not affect the cardiac electrical activity in rabbits, while stimulation with 35V will lead to all pacing rhythm of the heart without affecting the cardiac electrical activity in rabbits

  1. The dying rabbit problem revisited

    OpenAIRE

    Oller, Antonio M.

    2007-01-01

    In this paper we study a generalization of the Fibonacci sequence in which rabbits are mortal and take more that two months to become mature. In particular we give a general recurrence relation for these sequences (improving the work in the paper Hoggatt, V. E., Jr.; Lind, D. A. "The dying rabbit problem". Fibonacci Quart. 7 1969 no. 5, 482--487) and we calculate explicitly their general term (extending the work in the paper Miles, E. P., Jr. Generalized Fibonacci numbers and associated matri...

  2. [Review] Mary Toft's Rabbit Tale

    OpenAIRE

    Jones, Emrys

    2013-01-01

    Originally broadcast in April 2011 and aired again by BBC Radio 4 this November, Mary Toft’s Rabbit Tale is a radio drama retelling the story of its titular fraudster’s brief notoriety. With a high-profile cast – including singer Will Young as Toft’s husband and Rupert Graves as man-midwife, John Howard – the play explores a number of issues related to the alleged rabbit births of 1726 and their impact on public discourse of the time.

  3. Histomorphometric evaluation of bone healing in rabbit fibular osteotomy model without fixation

    OpenAIRE

    Paixão Fabio B; Araújo Francisco P; Matos Marcos A

    2008-01-01

    Abstract Background Animal models of fracture consolidation are fundamental for the understanding of the biological process of bone repair in humans, but histological studies are rare and provide only qualitative results. The objective of this article is to present the histomorphometric study of the bone healing process using an experimental model of osteotomy in rabbit fibula without interference of synthesis material. Methods Fifteen rabbits were submitted to fibular osteotomy without any f...

  4. Levan-induced glomerulitis in rabbits: a possible role for direct complement activation in situ.

    OpenAIRE

    Stark, H.; Alkalay, A.; Ben-Bassat, M; Hazaz, B.; Joshua, H

    1985-01-01

    Since high-molecular-weight levan is known to reduce capillary permeability to large molecules, an experiment was designed to investigate whether this agent may attenuate the glomerulonephritis associated with acute serum sickness in rabbits. The study, in fact, demonstrated an enhancing effect of levan, which caused increased glomerular proliferative changes and leucocyte infiltration and, possibly, increased IgG deposition in this experimental model. In addition, rabbits injected only with ...

  5. Evaluation of the Effect of Andrographolide on Atherosclerotic Rabbits Induced by Porphyromonas gingivalis

    OpenAIRE

    Al Batran, Rami; Al-Bayaty, Fouad; Al-Obaidi, Mazen M. Jamil; Hussain, Saba F.; Mulok, Tengku Z.

    2014-01-01

    Epidemiologic evidence has demonstrated significant associations between atherosclerosis and Porphyromonas gingivalis (Pg). We had investigated the effect of andrographolide (AND) on atherosclerosis induced by Pg in rabbits. For experimental purpose, we separated thirty male white New Zealand rabbits into 5 groups. Group 1 received standard food pellets; Groups 2–5 were orally challenged with Pg; Group 3 received atorvastatin (AV, 5 mg/kg), and Groups 4-5 received 10 and 20 mg/kg of AND, resp...

  6. Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits

    OpenAIRE

    Kannan TP; Freeda Woon Faiza Woon Tai Keat; Widowati Witjaksono; Abdul Rani Samsudin

    2006-01-01

    Coral has been used as a bone substitute in many experimental studies. It has been proven to be biocompatible, biodegradable and easy to handle: and it has not been found to cause any inflammatory responses. The present study was undertaken to determine the cytotoxicity in terms of mitotic index as well as the clastogenic effect (chromosome aberration) of sea coral implantation in rabbits. The animals comprised of five male adult healthy New Zealand White (Oryctolagus cuniculus) rabbits. The ...

  7. Digestibility of diets containing an antibiotic, a probiotic and a prebiotic in growing rabbits

    OpenAIRE

    Euclides Braga Malheiros; Otto Mack Junqueira; Aderbal Cavalcante Neto; Jeffrey Frederico Lui; Joseli Alves Ferreira Zanato; Maria Cristina de Oliveira; Claúdio Scapinello

    2008-01-01

    This study aimed to evaluate the effects of a probiotic and/or prebiotic additive on the nutrient digestibility of diets for growing rabbits. Twenty 50-day-old rabbits were used. A completely randomized experimental design, with five treatments and four replicates each, was used. The applied treatments (T) were the following: T1 = negative control diet (no growth promoter); T2 = diet with antibiotic, positive control (0.05% zinc bacitracin); T3 = diet with probiotic (0.15% Bacillus subtillis ...

  8. EFFECT OF QUERCETIN ON HAEMATOLOGICAL PARAMETERS OF RABBITS: AGENDER COMPARISON

    Directory of Open Access Journals (Sweden)

    Peter Petruška

    2013-02-01

    Full Text Available The aim of the present study was to determine the effect of application of quercetin in various doses on selected haematological parameters of rabbit's blood.Adult female rabbits (n = 20 and male rabbits (n = 20 were used in experiment.Animals were divided into four groups (n=5 in each group, one control group (C and three experimental groups (E1, E2 and E3 in both sexes. Experimental groups received quercetin in injectable form (intramuscularly at 10 µg.kg-1 in E1 group, 100 µg.kg-1 in E2 group, and 1000 µg.kg-1 in E3 group for 90 days 3 times a week. Control group received injection water.Higher values in WBC (total white blood cell count, GRA (granulocytes, RBC (red blood cells count and HGB (haemoglobin in experimental groups in comparison with the control group were found,however without significant differences (P>0.05.Increase in HCT in all experimental male groups in comparison with the control group without significant differences (P>0.05 was observed.Other haematological parameters were not influenced by this natural antioxidant.

  9. Comparison of sympathetic nerve activity normalization procedures in conscious rabbits.

    Science.gov (United States)

    Burke, Sandra L; Lim, Kyungjoon; Moretti, John-Luis; Head, Geoffrey A

    2016-05-01

    One of the main constraints associated with recording sympathetic nerve activity (SNA) in both humans and experimental animals is that microvolt values reflect characteristics of the recording conditions and limit comparisons between different experimental groups. The nasopharyngeal response has been validated for normalizing renal SNA (RSNA) in conscious rabbits, and in humans muscle SNA is normalized to the maximum burst in the resting period. We compared these two methods of normalization to determine whether either could detect elevated RSNA in hypertensive rabbits compared with normotensive controls. We also tested whether either method eliminated differences based only on different recording conditions by separating RSNA of control (sham) rabbits into two groups with low or high microvolts. Hypertension was induced by 5 wk of renal clipping (2K1C), 3 wk of high-fat diet (HFD), or 3 mo infusion of a low dose of angiotensin (ANG II). Normalization to the nasopharyngeal response revealed RSNA that was 88, 51, and 34% greater in 2K1C, HFD, and ANG II rabbits, respectively, than shams (P < 0.05), but normalization to the maximum burst showed no differences. The RSNA baroreflex followed a similar pattern whether RSNA was expressed in microvolts or normalized. Both methods abolished the difference between low and high microvolt RSNA. These results suggest that maximum burst amplitude is a useful technique for minimizing differences between recording conditions but is unable to detect real differences between groups. We conclude that the nasopharyngeal reflex is the superior method for normalizing sympathetic recordings in conscious rabbits. PMID:26921439

  10. 无线遥测和刺激技术建立兔房颤模型的探索%Exploratory experimental study on rabbit model of atrial fibrillation by wireless telemetering and stimulation technology

    Institute of Scientific and Technical Information of China (English)

    宋磊; 陈颖敏; 张方亮; 罗章源; 张文赞; 何奔

    2014-01-01

    Objective To explore the establishment of a rabbit model of atrial fibrillation by wireless telemetering and stimulation technology.Method An implantable telemetering stimulator which was independently designed and devel-oped was hypodermically implanted in New Zealand rabbits.The implantable telemetering stimulator was made with the core development and design of MSP single-chip microcomputer of TI Corporation ( Texas Instruments) and RF wireless trans-ceiver chip CC2250 of TI Corporation.The design of the implantation system was optimized to cater to the exploratory ex-periment to establish atrial fibrillation model of New Zealand rabbits.The implanter was implanted into the abdominal sub-cutaneous tissue of the New Zealand rabbits, the collecting electrodes were placed in the oxter subcutaneous tissues of the left and the right upper extremities, and the two stimulating electrodes were sutured at the left auricle and the left atrium. The signals were collected and stimulated by the wireless transceiver.The I-lead ECG electrical signals were continuously monitored on the body surface by a Powerlab physiological recorder.High frequency ( >20 Hz) suprathreshold stimulus ( intensity 2 mA, pulse width 1 ms) was emitted by specialized stimulation software of a computer program by the interval ( stimulating for 2 s and pausing for 2 s) .In case of atrial fibrillation during intervals, the stimulation could be stopped by hand.In case of sinus rhythm, the stimulation could be continued.Results The implantable telemetering stimulator can work stably in vivo ( including collecting stimulated electrocardio signal and emitting stimulations) for 30 days.Atrial fibril-lation can be induced after stimulating in vivo of the New Zealand rabbits for 3 weeks, with a duration of >48 h.Conclu-sions Applying implantable telemetering stimulator can build a New Zealand istead of beagles model of atrial fibrillation which is more consistent with welfare optimization and substitution

  11. Blood brain barrier and brain tissue injury by Gd-DTPA in uremia-induced rabbits

    International Nuclear Information System (INIS)

    An experimental study was carried out to evaluate the morphological changes in the blood brain barrier and neighbouring brain tissue caused by Gd-DTPA in uremia-induced rabbits. Bilateral renal arteries and veins of ten rabbits were ligated. Gd-DTPA(0.2mmol/kg) was intravenously injected into seven rabbits immediately after ligation. After MRI, they were sacrificed 2 or 3 days after ligation in order to observe light and electron microscopic changes in the blood brain barrier and brain tissue. MRI findings were normal, except for enhancement of the superior and inferior sagittal sinuses on T1 weighted images in uremia-induced rabbits injected with Gd-DTPA. On light microscopic examination, these rabbits showed perivascular edema and glial fibrillary acidic protein expression: electron microscopic examination showed separation of tight junctions of endothelial cells, duplication/rarefaction of basal lamina, increased lysosomes of neurons with neuronal death, demyelination of myelin, and extravasation of red blood cells. Uremia-induced rabbits injected with Gd-DTPA showed more severe changes than those without Gd-DTPA injection. Injuries to the blood brain barrier and neighbouring brain tissue were aggravated by Gd-DTPA administration in uremia-induced rabbits. These findings appear to be associated with the neurotoxicity of Gd-DTPA

  12. Performance of growing rabbits fed increasing levels of discarded Salvia hispanica L. (chia) seed.

    Science.gov (United States)

    Rodríguez-Abello, Diana; Ramírez-Avilés, Luis; Navarro-Alberto, Jorge; Zamora-Bustillos, Roberto

    2016-06-01

    This experiment was carried out to evaluate the effect of including five levels of discarded chia seed (DCS) on growth, energy, and economic efficiency in rabbits. Fifty crossbred (New Zealand × Californian) weaning rabbits, weighing 798 ± 170 g, were randomly assigned to the following treatments: 0 % (control), 10 % (Ch10), 20 % (Ch20), 30 % (Ch30), and 40 % (Ch40) of DCS. Ten unsexed rabbits were allocated to each treatment and housed individually in cages; the experimental period was 6 weeks. There were no statistically significant differences between treatments in final body weight (2050 g), body weight gain (30 g/rabbit/day), and carcass weight (1010 g); however, feed intake (91 g/rabbit/day in Ch20 vs. 80 g/rabbit/day in Ch40), feed conversion ratio (2.77 in control and Ch40 vs. 2.95 in Ch10, Ch20, and Ch30), energy conversion ratio (32 MJ/kg in Ch20 vs. 29 MJ/kg on average in control, Ch10, Ch20, Ch30, and Ch40), and economic efficiency (358 % in control vs. 433 % in Ch40) showed differences (P < 0.05) between treatments. Discarded chia seed can be included up to 40 % in growing rabbit diets without any adverse effects on growth and carcass weight, and increased economic efficiency. PMID:26992735

  13. Confiabilidade de um método de análise histológica da degeneração discal experimental em coelhos Confiabilidad de un método para el análisis histológico de la degeneración discal experimental en conejos Reliability of a method for histological analysis of experimental disc degeneration in rabbits

    Directory of Open Access Journals (Sweden)

    Emiliano Neves Vialle

    2011-01-01

    Zealand fueron sometidos a procedimiento de inducción de la degeneración discal a través de punción directa de tres discos intervertebrales (DIV consecutivos con aguja 18G bajo anestesia. Al fin de dos meses, se colectó la columna vertebral completa de cada conejo y se procedió a la preparación de las piezas para análisis histológico de los discos intervertebrales (Experimentales y Controles, siendo coloreadas por el método hematoxilina-eosina. Las láminas histológicas fueron evaluadas cuanto a ocurrencia de degeneración a través del análisis de los siguientes criterios: presencia de vasos sanguíneos; presencia de protrusión del núcleo pulposo (NP a través del anillo fibroso (AF; y rotura de las fibras del AF. RESULTADOS: Al final de los procesos de eutanasia, remoción y preparación histológica de la columna, se obtuvieron 60 DIV viables para evaluación de degeneración. De este total, 25 piezas eran de DIV experimentales y 35 piezas de DIV control. La presencia de vaso sanguíneo fue observada en 18 de los 25 DIV degenerados, con concordancia de Kappa = 0,95 entre los observadores. La presencia de la extrusión del NP fue identificada en 19 de los 25 DIV experimentales, con concordancia de Kappa = 0,78 entre los observadores. Con relación a la rotura de las fibras del AF, se puede identificar la positividad en 24 de los 25 DIV degenerados, con concordancia entre los observadores de Kappa = 0,65. CONCLUSIÓN: Este modelo de evaluación histológica de la degeneración experimental del DIV se mostró viable, con alto grado de concordancia entre los observadores en la identificación de la degeneración discal.OBJECTIVE: To validate a method for histological evaluation of disc degeneration in rabbits based on the reliability of interobserver analysis. METHODS: Thirteen New Zealand white rabbits underwent a procedure to induce disc degeneration through direct puncture of three consecutive intervertebral discs (IVD with an 18G needle under anesthesia

  14. Rabbit Models of Ocular Diseases: New Relevance for Classical Approaches.

    Science.gov (United States)

    Zernii, Evgeni Y; Baksheeva, Viktoriia E; Iomdina, Elena N; Averina, Olga A; Permyakov, Sergei E; Philippov, Pavel P; Zamyatnin, Andrey A; Senin, Ivan I

    2016-01-01

    Over 100 million individuals are affected by irreversible visual impairments and blindness worldwide, while ocular diseases remain a challenging problem despite significant advances in modern ophthalmology. Development of novel drugs and drug delivery mechanisms, as well as advanced ophthalmological techniques requires experimental models including animals, capable of developing ocular diseases with similar etiology and pathology, suitable for future trials of new therapeutic approaches. Although experimental ophthalmology and visual research are traditionally performed on rodent models, these animals are often unsuitable for pre-clinical drug efficacy and safety studies, as well as for testing novel drug delivery approaches, e.g. controlled release of pharmaceuticals using intra-ocular implants. Therefore, rabbit models of ocular diseases are particularly useful in this context, since rabbits can be easily handled, while sharing more common anatomical and biochemical features with humans compared to rodents, including longer life span and larger eye size. This review provides a brief description of clinical, morphological and mechanistic aspects of the most common ocular diseases (dry eye syndrome, glaucoma, age-related macular degeneration, light-induced retinopathies, cataract and uveitis) and summarizes the diversity of current strategies for their experimental modeling in rabbits. Several applications of some of these models in ocular pharmacology and eye care strategies are also discussed. PMID:26553163

  15. Evaluation of the rabbit as a model for Chagas disease - II: histopathologic studies of the heart, digestive tract and skeletal muscle

    OpenAIRE

    Arnaldo Moreira da Silva; Luiz Eduardo Ramirez; Marlene Vargas; Edmundo Chapadeiro; Zigman Brener

    1996-01-01

    In order to investigate the value of the rabbit as an experimental model for Chagas' disease, seventy one animals were inoculated with different Trypanosoma cruzi strains and routes. The rabbits were submitted to necropsy in acute (earlier than three months of infection), recent chronic (three to six months) and late chronic (later than six months) phases. Myocarditis, generally focal and endomysial, occurred in 94.1%, 66.7% and 70.8% of the infected rabbits respectively in the acute, recent ...

  16. Intra-amniotic administration of exogenous pulmonary surfactant for improving in lung maturity of fetal rabbits with intrauterine infection caused by premature rupture of membranes

    OpenAIRE

    Liu, Jing; Wu, Jing; Yang, Na; Feng, Zhichun

    2011-01-01

    This study was to investigate the effect of intra-amniotic administration of pulmonary surfactant (PS) on lung maturation in conditions of experimentally induced premature rupture of membranes (PROM) and intrauterine infection of rabbits. To establish animal (rabbit) models of intrauterine infection caused by PROM, E. coli was intrauterinely injected in 24-and 26-day pregnant animals. Twenty healthy pregnancy adult Japanese white rabbits were divided into three groups: the infection group (8 ...

  17. Innervation of the rabbit cardiac ventricles.

    Science.gov (United States)

    Pauziene, Neringa; Alaburda, Paulius; Rysevaite-Kyguoliene, Kristina; Pauza, Audrys G; Inokaitis, Hermanas; Masaityte, Aiste; Rudokaite, Gabriele; Saburkina, Inga; Plisiene, Jurgita; Pauza, Dainius H

    2016-01-01

    The rabbit is widely used in experimental cardiac physiology, but the neuroanatomy of the rabbit heart remains insufficiently examined. This study aimed to ascertain the architecture of the intrinsic nerve plexus in the walls and septum of rabbit cardiac ventricles. In 51 rabbit hearts, a combined approach involving: (i) histochemical acetylcholinesterase staining of intrinsic neural structures in total cardiac ventricles; (ii) immunofluorescent labelling of intrinsic nerves, nerve fibres (NFs) and neuronal somata (NS); and (iii) transmission electron microscopy of intrinsic ventricular nerves and NFs was used. Mediastinal nerves access the ventral and lateral surfaces of both ventricles at a restricted site between the root of the ascending aorta and the pulmonary trunk. The dorsal surface of both ventricles is supplied by several epicardial nerves extending from the left dorsal ganglionated nerve subplexus on the dorsal left atrium. Ventral accessing nerves are thicker and more numerous than dorsal nerves. Intrinsic ventricular NS are rare on the conus arteriosus and the root of the pulmonary trunk. The number of ventricular NS ranged from 11 to 220 per heart. Four chemical phenotypes of NS within ventricular ganglia were identified, i.e. ganglionic cells positive for choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), and biphenotypic, i.e. positive for both ChAT/nNOS and for ChAT/tyrosine hydroxylase. Clusters of small intensely fluorescent cells are distributed within or close to ganglia on the root of the pulmonary trunk, but not on the conus arteriosus. The largest and most numerous intrinsic nerves proceed within the epicardium. Scarce nerves were found near myocardial blood vessels, but the myocardium contained only a scarce meshwork of NFs. In the endocardium, large numbers of thin nerves and NFs proceed along the bundle of His and both its branches up to the apex of the ventricles. The endocardial meshwork of fine NFs was

  18. 声触诊组织定量技术评估兔慢性肾病肾纤维化的实验研究%Renal Fibrosis Assessment in Rabbits with Chronic Renal Nephropathy Using Virtual Touch Tissue Quantiifcation Technique:Experimental Study

    Institute of Scientific and Technical Information of China (English)

    陆敏; 杜联芳; 王迎春; 徐荣

    2014-01-01

    目的:建立新西兰兔慢性肾病模型,应用声触诊组织定量(VTQ)技术监测兔慢性肾病的发展,为VTQ评估慢性肾病肾纤维化提供实验依据。材料与方法选取38只健康新西兰大白兔,其中32只每天经耳缘静脉注射阳离子牛血清白蛋白(C-BSA)诱导慢性肾病模型,另外6只未注射C-BSA设为对照组。于0周、2周、4周、6周、8周采用VTQ测量兔肾皮质硬度,随后解剖兔,评估肾脏的病理变化,并分析VTQ参数与病理参数的相关性。结果兔肾皮质0周、2周、4周、6周、8周的VTQ值分别为(1.68±0.25)m/s、(1.70±0.31)m/s、(1.87±0.35)m/s、(2.19±0.31)m/s、(2.46±0.46)m/s,6周VTQ值与4周比较差异有统计学意义(P<0.05),8周VTQ值与4周、6周比较差异有统计学意义(P<0.05)。4周、6周、8周肾小球硬化指数分别为0.81±0.40、1.43±0.46、2.15±0.46,肾间质胶原纤维面积分别为(14.29±4.62)%、(26.28±11.06)%、(42.37±10.09)%,均明显高于0周、2周,且纤维化随病程进展逐渐增高,差异有统计学意义(P<0.05)。肾皮质VTQ与肾小球硬化指数、肾间质胶原纤维面积呈正相关(r=0.663、0.652, P<0.05)。结论VTQ可以检测到兔慢性肾病发展过程中肾皮质硬度增高,与肾纤维化呈正相关,提示VTQ可以成为评价肾纤维化的重要方法。%Purpose Chronic nephropathy model was established with New Zealand rabbit, and the development of chronic nephropathy was monitored using virtual touch tissue quantification (VTQ) technique, to provide the experimental basis for chronic renal fibrosis assessment using VTQ technique. Materials and Methods Thirty-eight healthy New Zealand white rabbits were selected, of which 32 were induced into chronic nephropathy model with daily intravenous ear vein injection of cationic bovine serum albumin (C-BSA), with the other 6 rabbits without C-BSA injection as control

  19. Nutritional studies on growing rabbits

    International Nuclear Information System (INIS)

    This work was carried out to study the effect of adding drinking water with either, copper sulfate, ascorbic acid or drinking cooled water on growth performance (live body weight,body weight gain, feed intake, feed conversion and water consumption), digestibility coefficients of nutrients, carcass traits, some physiological parameters and economical efficiency of growing NZW rabbits under Egyptian summer conditions. Ninety six weanling New Zealand White (NZW) male rabbits at five weeks of age and nearly similar average body weight (650.3 ±3.7 g) were randomly divided into eight treatment groups (twelve rabbits in each group), and then each group was subdivided into four replicates, each of three rabbits. The rabbits were assigned to drinking water as follow: the 1 st group was given fresh tap water without any additives as a control. The 2nd, 3rd and 4th groups were given tap fresh water supplemented with copper sulfate at levels of 40, 80 and 120 mg/L drinking water, respectively. The 5th, 6th and 7th groups were given tap fresh water supplemented with ascorbic acid at levels of 250, 500 and 750 mg/L drinking water, respectively. The 8th group was given cooled drinking water (CW) at 10-15 degree C. Results showed that supplementation of 40 or 80 mg copper sulfate/L or 500 mg ascorbic acid/L to heat-stressed rabbits drinking water improved final live body weight, body weight gain, daily water consumption, feed conversion ratio, performance index and economical efficiency. Hot carcass percentage was significantly (P<0.01) decreased with 80 mg/L copper sulfate and increased significantly (P<0.01) due to supplementation the drinking water with 250 mg ascorbic acid/L. Cooled water (10-15 degree C) improved significantly (P<0.01) each of final body weight, body weight gain, feed conversion ratio, performance index, economical efficiency and decreased significantly (P<0.01) each of hot carcass %, dressed weight %, heart %, total giblets %, rectal temperature and

  20. Comparison of bioavailability and pharmacokinetics of diclofenac sodium and diclofenac potassium in normal and dehydrated rabbits

    Institute of Scientific and Technical Information of China (English)

    Mahmood AHMAD; Muhammad IQBAL; Ghulam MURTAZA

    2009-01-01

    Two different salts of diclofenac, diclofenac sodium and dielofenae potassium, in tablet dosage form were tested for their bioavailability and disposition kinetics in a group of eighteen rabbits in normal and experimentally induced dehydrated conditions with a wash out period of 7 days between both stages of study. Biochemical and physiological parameters were also measured in both normal and dehydrated states. Diclofenac levels in plasma were determined using a validated reversed phase HPLC method. Primary kinetic parameters i.e. AUC0-∞, Cmax, Tmax and other disposition kinetics were obtained with non-compartmental procedure. Biochemical parameters i.e. packed cell volume, plasma glucose and total lipid concentration in dehydrated rabbits increased significantly. Plasma concentration of diclofenac sodium and diclofenac potassium decreased significantly in water deprived rabbits. In comparison, diclofenac potassium in normal and dehydrated state of the same group of rabbits showed a significantly increased plasma concentration when compared with diclofenac sodium.

  1. Oral administration of myostatin-specific recombinant Saccharomyces cerevisiae vaccine in rabbit.

    Science.gov (United States)

    Liu, Zhongtian; Zhou, Gang; Ren, Chonghua; Xu, Kun; Yan, Qiang; Li, Xinyi; Zhang, Tingting; Zhang, Zhiying

    2016-04-29

    Yeast is considered as a simple and cost-effective host for protein expression, and our previous studies have proved that Saccharomyces cerevisiae can deliver recombinant protein and DNA into mouse dendritic cells and can further induce immune responses as novel vaccines. In order to know whether similar immune responses can be induced in rabbit by oral administration of such recombinant S. cerevisiae vaccine, we orally fed the rabbits with heat-inactivated myostatin-recombinant S. cerevisiae for 5 weeks, and then myostatin-specific antibody in serum was detected successfully by western blotting and ELISA assay. The rabbits treated with myostatin-recombinant S. cerevisiae vaccine grew faster and their muscles were much heavier than that of the control group. As a common experimental animal and a meat livestock with great economic value, rabbit was proved to be the second animal species that have been successfully orally immunized by recombinant S. cerevisiae vaccine after mice. PMID:27005809

  2. Sentinel Node Mapping of VX2 Carcinoma in Rabbit Thigh with CT Lymphography Using Ethiodized Oil

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Yoon Jin; Kim, Young Hoon; Lee, Kyoung Ho; Park, Ji Hoon [Department of Radiology, Seoul National University Bundang Hospital, Seongnam 463-707 (Korea, Republic of); Lee, Hye Seung [Department of Pathology, Seoul National University Bundang Hospital, Seongnam 463-707 (Korea, Republic of); Jung, Seung Chai [Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul 110-744 (Korea, Republic of); Joo, Seung-Moon [Department of Radiology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 135-720 (Korea, Republic of)

    2014-07-01

    To assess the feasibility of computed tomography (CT) lymphography using ethiodized oil for sentinel node mapping in experimentally induced VX2 carcinoma in the rabbit thigh. This experiment received approval from the institutional animal use and care administrative advisory committee. Twenty-three rabbits with VX2 carcinoma in the thigh underwent CT before and after (1 hour, 2 hour) peritumoral injection of 2 mL ethiodized oil. After the CT examination, sentinel nodes were identified by peritumoral injection of methylene blue and subsequently removed. The retrieved sentinel and non-sentinel lymph nodes were investigated with radiographic and pathologic examinations. Based on the comparison of CT findings with those of radiographic and pathologic examinations, the diagnostic performance of CT for sentinel node identification was assessed. All 23 rabbits showed 53 ethiodized oil retention nodes on post-injection CT and specimen radiography, and 52 methylene blue-stained nodes at the right femoroiliac area. Of the 52 blue-stained sentinel nodes, 50 nodes demonstrated ethiodized oil retention. Thus, the sentinel node detection rate of CT was 96% (50 of 52). On pathologic examination, 28 sentinel nodes in 17 rabbits (nodes/rabbit, mean ± standard deviation, 1.7 ± 0.6) harbored metastasis. Twenty seven of the 28 metastatic sentinel nodes were found to have ethiodized oil retention. Computed tomography lymphography using ethiodized oil may be feasible for sentinel node mapping in experimentally induced VX2 carcinoma in the rabbit thigh.

  3. Sentinel Node Mapping of VX2 Carcinoma in Rabbit Thigh with CT Lymphography Using Ethiodized Oil

    International Nuclear Information System (INIS)

    To assess the feasibility of computed tomography (CT) lymphography using ethiodized oil for sentinel node mapping in experimentally induced VX2 carcinoma in the rabbit thigh. This experiment received approval from the institutional animal use and care administrative advisory committee. Twenty-three rabbits with VX2 carcinoma in the thigh underwent CT before and after (1 hour, 2 hour) peritumoral injection of 2 mL ethiodized oil. After the CT examination, sentinel nodes were identified by peritumoral injection of methylene blue and subsequently removed. The retrieved sentinel and non-sentinel lymph nodes were investigated with radiographic and pathologic examinations. Based on the comparison of CT findings with those of radiographic and pathologic examinations, the diagnostic performance of CT for sentinel node identification was assessed. All 23 rabbits showed 53 ethiodized oil retention nodes on post-injection CT and specimen radiography, and 52 methylene blue-stained nodes at the right femoroiliac area. Of the 52 blue-stained sentinel nodes, 50 nodes demonstrated ethiodized oil retention. Thus, the sentinel node detection rate of CT was 96% (50 of 52). On pathologic examination, 28 sentinel nodes in 17 rabbits (nodes/rabbit, mean ± standard deviation, 1.7 ± 0.6) harbored metastasis. Twenty seven of the 28 metastatic sentinel nodes were found to have ethiodized oil retention. Computed tomography lymphography using ethiodized oil may be feasible for sentinel node mapping in experimentally induced VX2 carcinoma in the rabbit thigh

  4. A clinically relevant BTX-A injection protocol leads to persistent weakness, contractile material loss, and an altered mRNA expression phenotype in rabbit quadriceps muscles.

    Science.gov (United States)

    Fortuna, Rafael; Vaz, Marco A; Sawatsky, Andrew; Hart, David A; Herzog, Walter

    2015-07-16

    Botulinum toxin type-A (BTX-A) injections have become a common treatment modality for patients suffering from muscle spasticity. Despite its benefits, BTX-A treatments have been associated with adverse effects on target muscles. Currently, application of BTX-A is largely based on clinical experience, and research quantifying muscle structure following BTX-A treatment has not been performed systematically. The purpose of this study was to evaluate strength, muscle mass, and contractile material six months following a single or repeated (2 and 3) BTX-A injections into the quadriceps femoris of New Zealand white rabbits. Twenty three skeletally mature rabbits were divided into four groups: experimental group rabbits received 1, 2, or 3 injections at intervals of 3 months (1-BTX-A, 2-BTX-A, 3-BTX-A, respectively) while control group rabbits received volume-matched saline injections. Knee extensor strength, quadriceps muscle mass, and quadriceps contractile material of the experimental group rabbits were expressed as a percentage change relative to the control group rabbits. One-way ANOVA was used to determine group differences in outcome measures (α=0.05). Muscle strength and contractile material were significantly reduced in experimental compared to control group rabbits but did not differ between experimental groups. Muscle mass was the same in experimental BTX-A and control group rabbits. We concluded from these results that muscle strength and contractile material do not fully recover within six months of BTX-A treatment. PMID:26087882

  5. White Rabbit Status and Prospects

    CERN Document Server

    Serrano, J; Cattin, M; van der Bij, E; Wlostowski, T; Daniluk, G; Lipinski, M; Beck, D; Hoffmann, J; Kreider, M; Prados, C; Rauch, S; Terpstra, W W; Zweig, M

    2014-01-01

    The White Rabbit (WR) project started off to provide a sequencing and synchronisation solution for the needs of CERN and GSI. Since then, many other users have adopted it to solve problems in the domain of distributed hard realtime systems. The paper discusses the current performance of WR hardware, along with present and foreseen applications. It also describes current efforts to standardise WR under IEEE 1588 and recent developments on reliability of timely data distribution, finishing with an outline of future plans.

  6. Osteoblastic Osteosarcoma in a Rabbit

    OpenAIRE

    Ishikawa, Megumi; Kondo, Hirotaka; Onuma, Mamoru; Shibuya, Hisashi; Sato, Tsuneo

    2012-01-01

    An osteosarcoma developed in the tarsal joint region involving the distal tibia of a domestic rabbit (Oryctolagus cuniculus). Micrometastases were present in the lungs. Histologically the tumor was composed of ovoid to short-spindle cells with abundant giant cells, producing irregular islands of osteoids. The tumor cells were immunopositive with antiosteocalcin monoclonal antibody, consistent with their derivation from osteoblasts. According to review of 10 published cases, productive osteobl...

  7. Functional domains of rabbit thrombomodulin.

    OpenAIRE

    Bourin, M C; Boffa, M C; Björk, I.; Lindahl, U

    1986-01-01

    Thrombomodulin isolated from rabbit lung was separated by ion-exchange chromatography on DEAE-cellulose into a retarded (acidic) and a nonretarded (nonacidic) fraction. Both fractions contained the cofactor required for the activation of protein C. In addition, the acidic fraction (but not the nonacidic fraction) prevented the clotting of fibrinogen by thrombin ("direct" anticoagulant activity) and accelerated the inhibition of thrombin by antithrombin (effect corresponding to 2-10 internatio...

  8. Welfare aspects in rabbit rearing and transport

    OpenAIRE

    Claudio Cavani; Massimiliano Petracci; Fabio Luzi; Marina Verga

    2010-01-01

    The review starts with the description of the rabbits’ (Oryctolagus cuniculus) main habits and the current situation concerning the rabbit husbandry and management systems, as well as their effects on the welfare of these animals. As far as the intensive rabbit husbandry systems are concerned, the main problems are related to the time since rabbits have been domesticated and their adaptive capacity and coping styles as respects the farming environment and management systems. Both these ...

  9. Assessment of propofol anesthesia in the rabbit

    OpenAIRE

    Campos, Sónia Patrícia Seabra

    2010-01-01

    The growing interests in propofol as an intravenous anesthetic agent and in particular to its use in rabbits were the motivation for this work. The rabbit is the third most common pet, and is also used as a biomedical research model in a wide range of science branches such as pharmacology, toxicology, anesthesiology and surgery. The main goal of this study was to explore the effects of Total Intravenous Anesthesia (TIVA) with propofol in rabbits. Different infusion rates were administrated...

  10. Identification of Rabbit Myostatin Gene Polymorphisms

    OpenAIRE

    T. I. Amalianingsih; B Brahmantiyo; Jakaria

    2015-01-01

    The existence of selection on the rabbits with potential for meat has only been seen from phenotypic aspects including performance and productivity, while the molecular genetic studies are still very rare. One of the candidate genes for meat production traits in rabbit is myostatin. Totally 50 blood samples of male rabbits from Rex, Satin, Reza (crossing from Rex and Satin), Flemish Giant and FZ3 (crossing from Flemish Giant and Reza) breed were used at Indonesian Research Institute for Anima...

  11. Bacteriocin-producing Enterococci from Rabbit Meat

    OpenAIRE

    Szabóová, R.; Lauková, A.; Simonová, M.P,; Strompfová, V.; Chrastinová, L.

    2012-01-01

    Aims: Enterococci are lactic acid bacteria belonging to the division Firmicutes. They occur in different ecosystems, rabbits including. Enterococci can possess probiotic properties and produce antimicrobial substances-bacteriocins. Rabbit meat as nutritionally healthy food offers novel source to study bacteriocin-producing and/or probiotic enterococci. Methodology and results: Enterococci were detected from rabbit meat samples (42). Most of the isolates were allotted to the species Enterococ...

  12. Structural changes in the lengthened rabbit muscle

    OpenAIRE

    Pap, Károly; Berki, Sándor; Shisha, Tamás; Kiss, Sándor; Szőke, György

    2008-01-01

    This study evaluated the histological changes in muscle tissue after limb lengthening in skeletally mature and immature rabbits and assessed the most vulnerable level of striated muscle. Twenty-three male domestic white rabbits, divided into six groups, were operated on and different lengthening protocols were used in the mature and immature rabbits. The histopathological changes were analysed by a semi-quantitative method according to the scoring system of Lee et al. (Acta Orthop Scand 64(6)...

  13. Ultrasound Anatomical Visualization of the rabbit liver

    OpenAIRE

    Kamelia Dimcheva Stamatova-Yovcheva; Rosen Dimitrov; David Yovchev; Krassimira Uzunova; Rumen Binev

    2014-01-01

    The topic was to investigate the anatomical features of the rabbit liver by two- and three-dimensional ultrasonography. Eighteen sexually mature healthy clinically New Zealand rabbits aged eight months were studied. Two-dimensional ultarsonographic anatomical image of the rabbit liver presented it in the cranial abdominal region as a relatively hypoechoic finding. Its contours were regular and in close contact with the hyperechoic diaphragm. Liver parenchyma was heterogeneous. The gall bladde...

  14. Characterization of Rabbit CD5 Isoforms

    OpenAIRE

    Pospisil, Richard; Kabat, Juraj; Mage, Rose G.

    2009-01-01

    Previously described polyclonal or monoclonal antibodies (mAb) to rabbit CD5, raised against expressed recombinant protein or peptides, recognize CD5 on most rabbit B cells. The mAb KEN-5 was originally reported to recognize rabbit CD5. However, KEN-5 binds almost exclusively to T cells and only to a minor population of B cells. We show here that by Enzyme-linked Immunosorbent Assay (ELISA), KEN-5 binds to recombinant rabbit CD5. This interaction is partially inhibited by polyclonal goat anti...

  15. Physiological and biochemical changes after boldenone injection in adult rabbits.

    Science.gov (United States)

    Tousson, Ehab; El-Moghazy, Mostafa; Massoud, Ahmed; El-Atrash, Afaf; Sweef, Osama; Akel, Amani

    2016-01-01

    Boldenone (BOL) is an androgenic steroid that improves the growth and food conversion in food-producing animals. In most countries worldwide, this anabolic steroid is forbidden for human uses and meat production as it was developed for veterinary use. Recently, BOL is used by bodybuilders in both off season and pre-contest, where it is well known for increasing vascularity while preparing for a bodybuilding contest. The present study was designed to investigate the physiological and biochemical changes in rabbits after injection with the growth promoter BOL. A total of 32 adult New Zealand rabbits were divided into four groups, where the control group includes animals that were injected intramuscularly with olive oil and dissected after 3 weeks. The remaining three experimental groups included animals that received one, two and three intramuscular injections of 5 mg/kg body weight BOL, respectively, and were dissected after 3, 6 and 9 weeks, respectively. The animals from practice appeared healthy and did not show clinical signs of disease and none of the rabbits died during the experimental period. Serum total protein, globulin, alanine aminotransferase, asparate aminotransferase, urea, creatinine, testosterone, luteinizing hormone and follicle-stimulating hormone levels were significantly increased while serum direct bilirubin, albumin and albumin/globulin ratio were significantly decreased (p bodybuilders who suffer from infertility, renal and hepatic alterations following injection with some drugs as steroids (BOL) to build muscles. PMID:24081634

  16. Relationship between osteocyte apoptosis and orbital bone development in rabbits

    Institute of Scientific and Technical Information of China (English)

    MA Jian-min; LI Zhi-hui

    2004-01-01

    Objective To investigate whether the osteocyte apoptosis exists in orbital bones and to discuss its effect on the orbital development.Methods Seven young Newzealand white rabbits were selected as experimental animals.At two-month-old ,all rabbits were killed and then zygomas were made into paraffin and electron microscope sections after they were decalcified.Apoptosis of osteocytes was observed by light microscope and transmission electron microscopes and detected by TUNEL staining.Results The classical apoptosis of osteocytes was found under light and transmission electron microscopes.Apoptosis of osteocytes was diffused irregularly in the zygomatic tissue. Conclusion Osteocyte can apoptosis and it may participate in the development of the bony orbit.

  17. 二巯基丁二酸修饰的Fe3O4纳米磁液联合碘油动脉栓塞热疗治疗兔VX2肝癌%Treating the experimental rabbit VX2 hepatic tumor with arterial embolization hyperthermia with DSMA-Fe3O4 nano-sized magnetic fluid and lipiodol

    Institute of Scientific and Technical Information of China (English)

    余超; 冯耀良; 马明; 臧汉杰; 祖庆泉; 顾宁

    2012-01-01

    Objective To evaluate the efficacy of treating the experimental rabbit VX2 hepatic tumor with arterial embolization hyperthermia with DSMA-Fe3O4 nano-sized magnetic fluid and lipiodol. Methods Twenty-five rabbits were implanted with VX2 hepatic tumors into the left hepatic , lobes. Then they were randomly divided into five groups of A(control), B(lipiodol embolization), C(combined use of DSMA-Fe3()4 nano-sized magnetic fluid and lipiodol embolization) .EXDSMA-Fe3()4 nano-sized magnetic fluid embolization hyperthermia), and E( combined use of DSMA-Fe3()4 nano-sized magnetic fluid and lipiodol embolization hyperthermia). The volume of the xenografts was measured by CT scanning on the 14th day. The transarterial embolization was conducted with different emboliaztion agents mentioned above. The rabbits in groups of D and E accepted hyperthermia after embolizatioa The volume of the xenografts was followed-up by CT scanning on the 7* and 14th day after treatment. Then some rabbits of each group were sacrificed and the liver, spleen, kidney and lungs were removed for histopathology examination. Meanwhile, alanine aminotransferase ( ALT) and aspartate aminotransaminase( AST) of all rbbits were measured one day before embolization and on the 1st, 3rd and 7th day after therapy, respectively. Results All rabbit models with VX2 hepatic xenografts were successfully established. The differences in the tumor volumes, ALT and AST levels before treatment and on the 7th day after treatment were not statistically significant among 5 groups. On the 14th day after treatment, the tumor size in group E was decreased by 26. 7% compared to that before treatment, which in groups of B,C and D was increased by 200. 7%, 209. 4% and 422. 5%, respectively. Conclusion Arterial embolization hyperthermia with a combination of DSMA-Fe3O4 nano-sized magnetic fluid and lipiodol is effective, feasible and safe in treating VXz hepatic xenografts in rabbits.%目的 评估二巯基丁二酸修饰的Fe3O4

  18. 多巴胺对兔实验性形觉剥夺性近视形成的影响%Effects of dopamine on experimentally form deprived myopia in rabbits

    Institute of Scientific and Technical Information of China (English)

    高前应; 高如尧; 卢佩勇; 蒙艳春; 朱涛; 李力; 王培杰; 郭延奎

    2001-01-01

    目的 研究多巴胺对实验性近视形成的影响. 方法 7 日龄幼兔52只,分A, B, C, 3组. A组20只,单纯缝合眼睑;B组16只,缝合眼睑+玻璃体内 注射多巴胺;C组16只,缝合 眼睑+玻璃体注射生理盐水. 均以右眼为实验眼,左眼为对照眼. 第60日用A超测量每组双眼 的前房深度、晶体厚度、玻璃体腔长度和眼轴长度,并计算玻璃体腔长度/眼轴长度,同 时进行病理学观察. 结果 A,C两组中实验眼的玻璃体腔长度、眼轴长度 和玻璃体腔长度 /眼轴长度分别为(7.9±0.6) mm, (15.4±0.6) mm, 0.52±0.03; (7.3±0.5) mm, (15. 1±0.7) mm , 0.49±0.02; 对照眼则为(7.2±0.8) mm, (14.5±0.7) mm, 0.48±0.02; (6.8±0.4) m m, (14.6±0.6) mm, 0.46±0.02. 两眼间的差异有高度显著的统计学意义(P< 0.001),前房深度和晶体厚度 无统计学差异,而B组均无统计学差异. 巩膜胶原纤维在A, C两组中明显变细,而在B组中改 变较轻. 结论 形觉剥夺能导致眼球的轴性延长,玻璃体腔延长和VCL/AL 增大是其 形态学原因,巩膜纤维的变细、延长是其病理学原因之一,而多巴胺能部分阻止这些改变.%AIM To study effects of dopamine on form deprived myopia. [ WTHZ〗METHODS Fi fty-two of seven-day-old neonatal rabbits were divided into group A, B and C and monocularly deprived of pattern vision by suturation of right eyelids. I n group A (n=20), no drug was used, while rabbits in group B and C received a series of intravitreal injection of dopamine (n=16) or saline vehicle (n =16) with MD, respectively, the left eye were employed as control groups. Anterior chamb er depth (ACD), lenticular thickness (LT), vitreous chamber length (VCL) and axi al length (AL) of each eye were determined by means of A-scan ultrasonography on day 60, VCL/AL was calculated and pathologic changes were also observed. RESULTS VCL, AL and VCL/AL of deprived eyes in group A and C were

  19. Transgenic Mouse Bioassay: Evidence That Rabbits Are Susceptible to a Variety of Prion Isolates.

    Directory of Open Access Journals (Sweden)

    Enric Vidal

    2015-08-01

    Full Text Available Interspecies transmission of prions is a well-established phenomenon, both experimentally and under field conditions. Upon passage through new hosts, prion strains have proven their capacity to change their properties and this is a source of strain diversity which needs to be considered when assessing the potential risks associated with consumption of prion contaminated protein sources. Rabbits were considered for decades to be a prion resistant species until proven otherwise recently. To determine the extent of rabbit susceptibility to prions and to assess the effects of passage of different prion strains through this species a transgenic mouse model overexpressing rabbit PrPC was developed (TgRab. Intracerebral challenges with prion strains originating from a variety of species including field isolates (ovine SSBP/1 scrapie, Nor98- scrapie; cattle BSE, BSE-L and cervid CWD, experimental murine strains (ME7 and RML and experimentally obtained ruminant (sheepBSE and rabbit (de novo NZW strains were performed. On first passage TgRab were susceptible to the majority of prions (Cattle BSE, SheepBSE, BSE-L, de novo NZW, ME7 and RML tested with the exception of SSBP/1 scrapie, CWD and Nor98 scrapie. Furthermore, TgRab were capable of propagating strain-specific features such as differences in incubation periods, histological brain lesions, abnormal prion (PrPd deposition profiles and proteinase-K (PK resistant western blotting band patterns. Our results confirm previous studies proving that rabbits are not resistant to prion infection and show for the first time that rabbits are susceptible to PrPd originating in a number of other species. This should be taken into account when choosing protein sources to feed rabbits.

  20. Neuronal quantity and ultrastructure in extracorticospinal tract of newborn rabbits with cerebral palsy

    Institute of Scientific and Technical Information of China (English)

    Xiaojie Li; Ying Yang; Zhimei Jiang; Lanrnin Guo; Jing Gao

    2006-01-01

    BACKGROUND: The liver enzyme system and blood brain barrier function of newborn rabbits are incomplete.High level of bilirubin can lead to cerebral palsy (CP) of newborn rabbits. The pathological and ultrastructural changes of brains of immature rabbits may differ from those of mature rabbits.OBJECTIVE: To observe the changes in dopaminergic neuron amount and ultrastructure in the extracorticospinal tract of animal models of CP induced by hyperbilirubinemia.DESIGN: A randomized controlled observation.SETTING: Rehabilitation Medical College of Jiamusi University; Laboratory for Pediatric Neurology Rehabilitation of Jiamusi University.MATERIALS: Thirty newborn 2- to 5- day inbred Japanese rabbits, weighing about 50 g, of either gender, were provided by Experimental Animal Center, Jilin University. Bilirubin and tyrosine hydroxylase (TH) antibody were purchased from Sigma Corporation, USA.METHODS: This experiment was carried out in the laboratory for children neuro-rehabilitation, Jiamusi University between September 2002 and December 2005. ①Thirty rabbits were randomly divided into 2groups: control group (n =10) and model group (n =20). ②Bilirubin of 100 mg/kg was given to the rabbits of model group through intraperitoneal injection, once every other day, 3 times totally; The same amount of normal saline was given to the rabbits of control group. After injection, the newborn rabbits had been fed by maternal milk for 45 days. ③TH-positive neurons in substantia nigra and corpora striatum in each area of0.15 mm ×0.15 mm were counted. Uitrastructures of substantia nigra and corpora striatum were observed under the electron miscroscope. The concentration of bilirubin in brain tissue of CP rabbits of model group was measured with high performance liquid chromatogram.MAIN OUTCOME MEASURES: Quantitative analysis results and ultrastructure of TH-positive neurons in substantia nigra and corpora striatum in two groups.RESULTS: Thirty newborn rabbits were involved, 6

  1. EFFECT OF ORGANIC TURMERIC (Curcuma longa) FEEDING ON TESTICULAR HISTOLOGY OF RABBITS EXPOSED TO ULTRAVIOLET RADIATION

    OpenAIRE

    Togun, V.A.; Amao, O.A.; Adebisi, J.A.; Okwusidi, J.I.; Williams, O. S.

    2014-01-01

    Thirty six male rabbits of 450-600g weight range fed with mostly organic ingredient feed, were weight-balanced into six treatment groups that received rabbit diets either without (D1) or with organic turmeric (D2) at varying periods within a nine- week experimental period. The treatments were T0, T1, T2, T3, T4 and T5 for control, D2 plus exposure to Ultraviolet (UV) radiation, D2 until radiation, D2 after radiation, D1 throughout the experimental period with UV radiation and D2 throughout th...

  2. Characterization of a novel alphaherpesvirus associated with fatal infections of domestic rabbits

    International Nuclear Information System (INIS)

    A virus was found to be associated with a severe disease affecting rabbits on a farm near Anchorage, Alaska. Extracts from the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin, rabbit kidney, and Vero cells. Examination of the infectious agent by electron microscopy revealed an icosahedral nucleocapsid surrounded by an envelope with a diameter of about 120 nm, suggesting that it was a herpesvirus. The viral genome was determined to be composed of double-stranded DNA of 120-130 kbp. PCR using degenerate primers to conserved herpesvirus genes was used to amplify sequences from purified viral DNA. Sequencing of these products allowed the design of specific primers so that complete sequence data for a number of genes could be determined. Analysis of these data indicated that the virus is most closely related to bovine herpesvirus 2. The next most closely related viruses are human herpesviruses 1 and 2, and a number of cercopithecine herpesviruses. Experimental exposure of domestic rabbits to the isolate resulted in severe clinical disease and necrosis in the spleen and lymph node. In addition, viral DNA was identified in a variety of tissues by PCR, consistent with a systemic infection. Taken together, these data suggest that this virus is highly pathogenic for domestic rabbits and belongs to the family Herpesviridae, subfamily Alphaherpesvirinae, genus Simplexvirus

  3. Effect of aguamiel (agave sap on hematic biometry in rabbits and its antioxidant activity determination

    Directory of Open Access Journals (Sweden)

    Héctor Silos-Espino

    2011-04-01

    Full Text Available In this work, we analysed the effect of aguamiel (sap from Agave salmiana on haematic biometry in rabbits as well as its antioxidant activity with the objective of determining its nutritional value. Nine male Landrace 55-day-old rabbits were sorted into three groups of three rabbits each, which had access to fresh aguamiel, boiled aguamiel and water instead of aguamiel. Commercial rabbit food was supplied ad libitum to each experimental unit. Fresh aguamiel promoted weight increases of about 13% by the seventh week. Haemoglobin counts increased by 4.5% and 9% when rabbits were fed with fresh and boiled aguamiel, respectively. Haematocrit counts increased from 2.6% to 5.3%. Mean corpuscular volume did not show a change. Mean corpuscular haemoglobin significantly increased up to 12% above that previously reported on rabbits. Observed Fe (fixed to transferrin, transferrin and ferritin content increased slightly when fresh aguamiel was included in the diet, as compared to the control. In bone, Fe increased 79.3% (boiled aguamiel while P increased 23.6% (fresh aguamiel. In antioxidant activity determination, the aguamiel showed 20% with respect to that found in coffee and/or grape juice (commercial beverages with a high antioxidant capability, 226.2 µmol gallic acid equivalent and 35.1 mg ascorbic acid in 250 mL, respectively. These results showed that aguamiel is an innocuous beverage to feed animals, conferring some beneficial properties, and it could be considered as a nutraceutical product.

  4. Subacute oral toxicity of endosulfan in male new zealand white rabbits.

    Science.gov (United States)

    Hatipoglu, F S; Gulay, M S; Balic, A; Yildiz-Gulay, O; Volkan, S

    2008-01-01

    ABSTRACT The present study was conducted using 6 to 8 month old New Zealand white male rabbits (nine rabbits per treatment group). Daily gavages of 3, 1.5, 0.75, or 0 mg endosulfan/kg BW in corn oil resulted in the death of five (55%), three (33%), zero (0%), and zero (0%) rabbits, respectively, in 30 days. All rabbits were monitored for any observable toxic symptoms throughout the experimental period (30 d) and they also were weighed weekly to monitor body weight gain. All deaths occurred within the first 3 weeks and nervous symptoms were observed only for a few minutes before death. Alterations recorded in hematological parameters within the groups (hemoglobin, packed cell volume, and total erythrocyte count) were due to endosulfan exposure. Serum alkaline phosphatase (ALP) and aspartate aminotransferase (AST) levels were significantly elevated in the 3 mg/kg dose group. Gross post-mortem and histopathological changes in various organs (lung, liver, kidney, and testes) of rabbits treated with endosulfan were observed with typical organochlorine dose-dependent signs of toxicity. Although some animals appeared to adjust to relatively high daily doses of endosulfan for 30 days, biochemical and histological evidence indicated varied liver and kidney damage relative to dosage administered to these animals. The current subacute (30 day) study suggested a NOAEL of 0.75 mg endosulfan/kg in New Zealand white rabbits. PMID:20020928

  5. Effects of ethyl-cyanoacrylate and octyl-cyanoacrylate on experimental corneal lesions in rabbits Efeitos do etil-cianoacrilato ou do octil-cianoacrilato sobre lesões corneais experimentais em coelhos

    Directory of Open Access Journals (Sweden)

    V.T. Barbosa

    2009-10-01

    Full Text Available The use of ethyl-cyanoacrylate and octyl-cyanoacrylate were clinically and histopathologically compared on the corneas of 36 rabbits after lamellar keratectomy (standardized diameter and depth. The animals were distributed into two groups, one for each type of adhesive. From each group, six subgroups were histopathologically evaluated on the 3rd, 7th, 14th, 21st, 30th, and 60th day post-operative. General (daily and ophthalmic (days 0, 1, 3, 5, 7, 14, 21, 30, 44, and 60 evaluations clinically indicated that there were significant differences for the variables water intake, attitude, blepharitis, corneal edema, and fluorescein test. The adhesive permanence time for octyl-cyanoacrylate (17.22 days was greater than that for ethyl-cyanoacrylate (7.66 days. With respect to the histopathological evaluation, corneal epithelization and collagen organization occurred without severe complications. However, treatment with ethyl-cyanoacrylate led to a moderate inflammatory reaction in the initial phases. With octyl-cyanoacrylate, re-epithelization and collagen organization proceeded more slowly with a discrete inflammatory reaction in the initial phases. From clinical and histopathologic points of view, octyl-cyanoacrylate showed advantages over ethyl-cyanoacrylate, whereas wound healing was achieved in both groups without major complications.Comparou-se o uso do etil-cianoacrilato e do octil-cianoacrilato em córneas de 36 coelhos após ceratectomia lamelar (diâmetro e profundidade padronizados. Os animais foram distribuídos em dois grupos, segundo o tipo de adesivo, e redistribuídos em seis subgrupos com três animais cada, para as avaliações histológicas aos 3, 7, 14, 21, 30 e 60 dias de pós-operatório. As avaliações clínicas gerais (diárias e as oftálmicas (dias 0, 1, 3, 5, 7, 14, 21, 30, 44 e 60, indicaram diferença entre os dois grupos, quanto ao consumo de água, atitude, blefarite, edema da córnea e teste da fluoresceína. O Tempo de

  6. 兔舌黏膜细胞的体外培养及生物学特性研究%Experimental study on biological character of rabbit lingual keratinocyees

    Institute of Scientific and Technical Information of China (English)

    顾正宇; 邹俊; 崔磊; 尹烁

    2009-01-01

    Objective To explore the cultural method of lingual keratinocytes and make preparations for further investigation in using lingual keratinocytes as a new choice of seed cells for the reconstruction of tissue engineered corneal epithelium.Methods Keratinocytes were enzymatically dissociated from lingual tissue specimens using 0.25%disepase and 0.05%trypsin.The biological characteristics of the cells were observed through phase microscope.Meanwhile.1ingual kemtinoeytes were immtmohistochemically labeled with broad spectrum keratin antibody (AE1/AF3).Results The cells revealed the form of "road metal" and could be passed for 3 or 4 generations.Immunohistochemistry indicated that lingual keratinocytes were positive for AE1/AE3 staining.Condusion Lingual kemtinocytes of rabbits can be cultured with KSFM in vitm and magnitude quantity can be attained,laying a favorable foundation for lingual keratinocytes as a new choice of seed cells for corneal epithelium reconstruction with tissue engineering.%目的 探讨舌黏膜细胞的培养方法,为进一步以舌黏膜细胞作为种子细胞构建组织工程化角膜上皮提供实验依据.方法 体外切取舌侧面和底面黏膜组织,经0.25%Dispase分离上皮层后,再经0.05%的胰酶分离为单个细胞,接种在无血清角质细胞培养液(KSFM)中,进行体外培养,并对其形态学以及生长曲线、传代特点、各代生长特点进行观察.同时,对体外培养获得的舌黏膜细胞进行广谱角蛋白免疫荧光鉴定.结果 舌黏膜细胞能够在无血清角质细胞培养液中稳定生长,呈现典型的"铺路石"状,细胞形态单一,可传至3~4代.细胞免疫荧光显示,体外培养获得的舌黏膜细胞AE1/AE3免疫荧光染色阳性.结论 应用无血清角质细胞培养液,可在短期内获得大量具有增殖能力的舌黏膜细胞,为进一步将其作为种子细胞构建组织工程化角膜上皮奠定了良好的基础.

  7. Viral skin diseases of the rabbit.

    Science.gov (United States)

    Meredith, Anna L

    2013-09-01

    This article describes the viral skin diseases affecting the domestic rabbit, the most important being myxomatosis. Transmission and pathogenesis, clinical signs, diagnosis, treatment, and control are described and the article will be of interest to veterinary practitioners who treat rabbits. Shope fibroma virus, Shope papilloma virus, and rabbitpox are also discussed. PMID:24018033

  8. Fate of transgenic deoxyribonucleic acid fragments in digesta and tissues of rabbits fed genetically modified soybean meal.

    Science.gov (United States)

    Morera, P; Basiricò, L; Ronchi, B; Bernabucci, U

    2016-03-01

    Numerous animal feeding studies have investigated the presence of DNA from transgenic plants in tissues from different animal species, but the data reported are sometimes controversial. The aim of this study was to investigate the presence of transgenic DNA (tDNA) in the digesta and tissues of a meat rabbit breed fed genetically modified (GM) soybean meal. Fifteen male New Zealand White rabbits were used for the experimental trial. Ten rabbits (treated group [TG]) were fed a mixed feed containing 10% GM soybean meal and 5 rabbits (control group [CG]) received a mixed feed containing conventional soybean meal, both from weaning (28 d of age) to slaughter (80 ± 3 d). Samples of blood, liver, kidney, heart, stomach, intestine (jejunum), lateral quadricep muscle, longissimus muscle, and perirenal adipose tissue were collected to assess the possible DNA transfer from GM feed to animal tissues. Samples of stomach contents and feces were also taken to study the degradability of ingested tDNA from feed in the digestive tract of rabbit. Moreover, samples of hair were collected to determine the possible environmental contamination from feed powders present on the farm. The DNA extraction was performed using specific genomic DNA kits. All samples were monitored, by using real-time PCR, for oligonucleotide primers and probes specific for the transgenic Roundup Ready soybean 40-3-2 and for the endogenous () gene. As an internal control of rabbit tissues, the presence of the () gene was used. In this study, no fragments of tDNA were detectable in tissue DNA samples of rabbits except in the extracted DNA from stomach digesta, feces, and hair of rabbits fed with GM soybean. Similar results were found for the reference gene, whereas the presence of the gene was detected in all rabbit tissues. The lack of tDNA of soybean in rabbit tissues represents an important result, which demonstrates that meat from rabbits fed a diet containing GM feed is as that derived from rabbits fed

  9. Rabbit Heating by Microwave Exposure at Various Ambient Temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Kolganova, Olga I.; Zhavoronkov, Leonid P.; Petin, Vladislav G. [Medical Radiological Research Center, Kaluga Region (Russian Federation); Kim, Jin Kyu [Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute, Jeongeup (Korea, Republic of)

    2010-09-15

    The potential ability of environmental temperature to enhance the effect of microwave radiation (7 GHz) was experimentally studied for rabbit heating after simultaneous application of both agents. The tested ambient temperatures (30 and 38 .deg. C) didn't exert a considerable influence upon rabbit heat homeostasis after the used duration of exposure (3 hours and 15 minutes, correspondingly). The synergistic interaction of microwave irradiation and ambient temperature was demonstrated for rabbit heating. Power flux density of microwave irradiation was shown to be a determinant of the synergistic interaction effectiveness. For the fixed ambient temperature (30 .deg. C), the synergism was shown to be observed only within a definite power flux density (0.100 mW·cm{sup -2}), inside of which there was an optimal intensity (20 mW·cm{sup -2}), which maximized the synergistic effect. Any deviation of the power flux density from the optimal value resulted in a reduction of the synergy. It is concluded that any assessment of the health or environmental risks should take into account the synergistic interaction between ambient temperature and microwave radiation.

  10. Rabbit Heating by Microwave Exposure at Various Ambient Temperatures

    International Nuclear Information System (INIS)

    The potential ability of environmental temperature to enhance the effect of microwave radiation (7 GHz) was experimentally studied for rabbit heating after simultaneous application of both agents. The tested ambient temperatures (30 and 38 .deg. C) didn't exert a considerable influence upon rabbit heat homeostasis after the used duration of exposure (3 hours and 15 minutes, correspondingly). The synergistic interaction of microwave irradiation and ambient temperature was demonstrated for rabbit heating. Power flux density of microwave irradiation was shown to be a determinant of the synergistic interaction effectiveness. For the fixed ambient temperature (30 .deg. C), the synergism was shown to be observed only within a definite power flux density (0.100 mW·cm-2), inside of which there was an optimal intensity (20 mW·cm-2), which maximized the synergistic effect. Any deviation of the power flux density from the optimal value resulted in a reduction of the synergy. It is concluded that any assessment of the health or environmental risks should take into account the synergistic interaction between ambient temperature and microwave radiation

  11. EFFECT OF HUMIC SUBSTANCES AND PROBIOTICS ON GROWTH PERFORMANCE AND MEAT QUALITY OF RABBITS

    Directory of Open Access Journals (Sweden)

    Vladimír Parkányi

    2012-04-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4  The aim of this work was determined effect of supplemental humic substances and probiotics on growth performance and meat quality of rabbits.  The growth performances were observed on two hundred and twelve growing rabbits assigned randomly by weight to two treatments. The treatments included: 1 Control group: in this group were rabbits fed with basal diet during all experiment (35th – 77th day, 2 experimental group: the animals were fed with basal diet+3kg/t humic substances – Humac Nature during whole experiment.  In this group was during fattening period (35th – 49th and 63rd – 70th days of age added to feed the probiotic preparation – Propoul (Lactobacillus fermentum CCM 7158 1x108 CFU 2 g per ten pieces. Body weight and feed intake were measured weekly in order to determine the average daily gain, average daily feed intake and gain/feed. The characteristics of meat quality were determined on twelve rabbit males at the age of 77 days, when the rabbits achieved average slaughter weight 2500g. Results of the whole experimental period showed that addition humic substances and probiotic preparation to the diet had positive effect (not significantly on intensity of growth live weight in the last phase of fattening period. The results of this study suggest that humic substances with probiotics might be utilized as a feed additive in the rabbit diet. It could not significantly improve growth performance and meat quality of rabbits.doi:10.5219/192 

  12. Erythrocyte Osmotic Fragility and Excitability Score in Rabbit fed Hibiscus Sabdariffa in Graded Level.

    Science.gov (United States)

    Adenkola, A Y; Oluremi, O I A

    2014-01-01

    This study was conducted for 10 weeks with the aim of investigating the erythrocyte membrane integrity as measured by erythrocyte osmotic fragility and excitability scores of rabbits fed graded level of Hibiscus sabdariffa calyx (HSC). Twenty weaners' rabbit of both sexes were used for the study and were placed on four experimental diets which contain the following percentages of HSC 0 %, 25 %, 50 %, 75 %, as feed additive and were added at 0 g, 62.5 g, 125 g, 187.5 g designated as T1, T2, T3 and T4 experimental diets. Excitability scores were measured weekly as described by Voisnet et al. (1997). At the end of the experiment, the rabbits were slaughtered by severing the jugular vein. A Blood sample (2 ml) was collected from each rabbit into sampled bottles, containing the Na EDTA as anticoagulant for hematological analysis. Packed cell volume (PCV) Haemoglobin concentration (Hb), Total red blood cell (RBC) count, Total leukocyte count as well as differential leukocyte was determined using standard method. The percentage haemolysis recorded at 0.3 % to 0.8 % was significantly (P < 0.05) higher in rabbits in T1 compared to the remaining 3 diets. The result of excitability score shows that rabbit on diet 1 and 2 had a lower value which was significantly (P < 0.05) lower than rabbits on diets 3 and 4 with a value of 65.5 ± 5.0 and 70.00 ± 5.50 % respectively. In conclusion this study demonstrated for the first time that chronic administration of HSC improves haematological parameters, brain mood and function as well as maintaining erythrocyte membrane integrity. PMID:26196576

  13. Rabbit model of intervertebral disc degeneration by external compression device characterized by X-ray, MRI, histology, and cell viability

    OpenAIRE

    Ismail Ismail; Hee H. Tak; James C. Goh; Wang S. Chang; Wong H. Kit

    2006-01-01

    Appropriate experimental animal models, which mimic the degenerative process occurring in human intervertebral disc (IVD) breakdown and can be used for new treatment studies such as tissue engineering or disc distraction are lacking. We studied the external compression device that used by Kroeber et al to create intervertebral disc degeneration in rabbit model characterized by X-ray, MRI, Histology, and Cell Viability. Ten NZW rabbit were randomly assigned to one of five groups. Intervertebra...

  14. Oxidative Damage and Mitochondrial Injuries Are Induced by Various Irrigation Pressures in Rabbit Models of Mild and Severe Hydronephrosis.

    Directory of Open Access Journals (Sweden)

    Zhixiu Cao

    Full Text Available We aimed to study whether tolerance to irrigation pressure could be modified by evaluating the oxidative damage of obstructed kidneys based on rabbit models experiencing different degrees of hydronephrosis.A total of 66 rabbits were randomly divided into two experimental groups and a control group. In the experimental groups, the rabbits underwent a surgical procedure inducing mild (group M, n=24 or severe (group S, n=24 hydronephrosis. In each experimental group, the rabbits were then randomly divided into 4 subgroups (M0-M3 and S0-S3 consisting of 6 rabbits each. Group 0 received no perfusion. Groups 1 through 3 were perfused with 20, 60 and 100 mmHg fluid, respectively. For the control group, after a sham operation was performed, the rabbits were divided into 4 subgroups and were perfused with fluid at 0, 20, 60 or 100 mmHg of pressure. Kidney injuries was evaluated by neutrophil gelatinase associated lipocalin (NGAL. Oxidative damage was assessed by analyzing superoxide dismutase (Mn-SOD activity, malondialdehyde (MDA levels, glutathione reductase (GR, catalase (CAT and peroxide (H2O2 levels, mitochondrial injuries was assessed by mitochondrial membrane potential (MMP, the mitochondrial ultrastructure and tubular cell apoptosis.In the experimental groups, all results were similar for groups 0 and 1. In group 2, abnormalities were observed in the S group only, and the kidneys of rabbits in group 3 suffered oxidative damage and mitochondrial injuries with increased NGAL, decreased Mn-SOD, GR and CAT,increased MDA and H2O2, lower levels of MMP, mitochondrial vacuolization and an increased apoptotic index.In rabbits, severely obstructed kidneys were more susceptible to oxidative damage and mitochondrial injury than mildly obstructed kidneys when subjected to higher degrees of kidney perfusion pressure.

  15. MR imaging of the denervated skeletal muscles in rabbits

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Hyung Guhn; Juhng, Seon Kwan; Lee, Sung Ah; Lee, Kang Mo; Kim, Seon Gu; Park, Dong Sik; Choi, See Sung; Roh, Byung Suk; Kim, Chang Guhn; Won, Jong Jin [Wonkwang University School of Medicine, Iksan (Korea, Republic of)

    1997-01-01

    To determine the time of magnetic resonance(MR) signal intensity changes in denervated skeletal muscle and to compare MR imaging with electromyography(EMG) in the evaluation of peripheral nerve injury. We evaluated MR imagings of denervated muscles after experimental transection of the sciatic nerve in five rabbits using 1.0T MR unit. MR imaging and EMG were performed 3 days and 1, 2 and 3 weeks after denervation T1-weighted images(T1-WI), T2-WI and Short Tau Inversion Recovery (STIR) images were obtained. The signal intensity (SI) of muscles in the denervated and normal sides were visually and quantitatively compared. After measuring the SI of the normal and abnormal areas, the time of SI change was determined when there was significant difference (P< 0.05) of SI between the normal and denervated sides. On STIR images, two of the five rabbits showed significant SI changes at the third day (P< 0.05) and all showed significant changes (P< 0.05) at the first week. On T2-WI, one rabbit showed significant SI changes at the third day, and all showed significant SI changes at the first week. On T1-WI, significant SI changes were seen in one rabbit at the second week and in one at the third. One week after denervation, all showed denervation potential on EMG. This study suggests that MR imaging using STIR images is a useful method in the evaluation of denervated muscle, and that MR signal changes of denervated muscle may precede EMG changes after denervation. To localize and to determine the severity of the peripheral nerve injury, future analysis of the distribution of abnormal MR SI in denervated muscles would be helpful.

  16. MR imaging of the denervated skeletal muscles in rabbits

    International Nuclear Information System (INIS)

    To determine the time of magnetic resonance(MR) signal intensity changes in denervated skeletal muscle and to compare MR imaging with electromyography(EMG) in the evaluation of peripheral nerve injury. We evaluated MR imagings of denervated muscles after experimental transection of the sciatic nerve in five rabbits using 1.0T MR unit. MR imaging and EMG were performed 3 days and 1, 2 and 3 weeks after denervation T1-weighted images(T1-WI), T2-WI and Short Tau Inversion Recovery (STIR) images were obtained. The signal intensity (SI) of muscles in the denervated and normal sides were visually and quantitatively compared. After measuring the SI of the normal and abnormal areas, the time of SI change was determined when there was significant difference (P< 0.05) of SI between the normal and denervated sides. On STIR images, two of the five rabbits showed significant SI changes at the third day (P< 0.05) and all showed significant changes (P< 0.05) at the first week. On T2-WI, one rabbit showed significant SI changes at the third day, and all showed significant SI changes at the first week. On T1-WI, significant SI changes were seen in one rabbit at the second week and in one at the third. One week after denervation, all showed denervation potential on EMG. This study suggests that MR imaging using STIR images is a useful method in the evaluation of denervated muscle, and that MR signal changes of denervated muscle may precede EMG changes after denervation. To localize and to determine the severity of the peripheral nerve injury, future analysis of the distribution of abnormal MR SI in denervated muscles would be helpful

  17. 9 CFR 354.124 - Quarantine of diseased rabbits.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Quarantine of diseased rabbits. 354... INSPECTION AND CERTIFICATION VOLUNTARY INSPECTION OF RABBITS AND EDIBLE PRODUCTS THEREOF Inspection Procedures; Ante-Mortem Inspections § 354.124 Quarantine of diseased rabbits. If live rabbits, which...

  18. Welfare aspects in rabbit rearing and transport

    Directory of Open Access Journals (Sweden)

    Claudio Cavani

    2010-01-01

    Full Text Available The review starts with the description of the rabbits’ (Oryctolagus cuniculus main habits and the current situation concerning the rabbit husbandry and management systems, as well as their effects on the welfare of these animals. As far as the intensive rabbit husbandry systems are concerned, the main problems are related to the time since rabbits have been domesticated and their adaptive capacity and coping styles as respects the farming environment and management systems. Both these aspects have implications in the present and future of rabbit rearing for different purposes. Examples are given on the effects of different housing and management systems on rabbit welfare, as well as examples of the ethological, physiological and productive indicators used to evaluate these effects. Transportation and, more generally, preslaughter phases including catching, fasting and lairage at the abattoir are considered major stressors for farmed rabbits and might have deleterious effects on health, well-being, performance, and finally, product quality. A general statement of the recent scientific studies considering the effects of pre-slaughter factors on physiological and productive measurements are reported. Finally, some indications in order to improve rabbit welfare, already present at the European level, are also outlined, together with the European Food Safety Authority opinions.

  19. Parasitic infections of wild rabbits and hares

    Directory of Open Access Journals (Sweden)

    Ilić Tamara

    2014-01-01

    Full Text Available The paper presents the most important parasitic infections of wild rabbits and hares, which harmful effect in this animal population is manifested as a gradual weakening of the immune system, reduction in fertility, weight loss and constant exhaustion. Order of Lagomorpha (hares or lagomorphs belongs to superorder of higher mammals which includes the family of rabbits (Leporidae which are represented in Europe as well as the family of whistleblowers (Ochotonidae which live only in North America and Northern regions of Asia. The most important representatives of Leporidae family are European hare (Lepus europeus and wild rabbit (Oryctolagus cuniculus. The most important endoparasitosis of hares and wild rabbits are: coccidiosis, encephalitozoonosis (nosemosis, toxoplasmosis, sarcocystosis, giardiasis, cryptosporidiosis, protostrongylosis, trichostrngylodosis, passalurosis, anoplocephalidosis, cysticercosis and fasciolosis. The most frequent ectoparasites of rabbits and wild hares are fleas, lice and ticks. Reduction in hare population, which is noticed in whole Europe including Serbia, is caused by changed living conditions, quantitatively and qualitatively insufficient nutrition, increased use of herbicides as well as various infectious diseases and the diseases of parasitic etiology. Since wild rabbits and hares pose a threat to health of domestic rabbits and people, knowledge of parasitic fauna of these wild animals is of extreme epizootiological and epidemiological importance.

  20. Relationship between expression of MMP-9 and presyrinx state of experimental syringomyelia of rabbits%基质金属蛋白酶-9的表达变化与实验性家兔脊髓空洞前状态的关系

    Institute of Scientific and Technical Information of China (English)

    申庆丰; 张庆俊; 田融; 夏英鹏; 徐天同; 夏刚

    2010-01-01

    目的 探讨家兔脊髓空洞症模型脊髓基质金属蛋白酶(MMP)-9的表达变化与脊髓空洞前状态中脊髓水肿形成的关系,评价强力霉素对脊髓空洞前状态水肿形成的抑制作用.方法 中国白兔96只,随机分为4组:Kaolin组、强力霉素组、生理盐水组、假手术组各24只.家兔在麻醉后,行经皮枕大池穿刺术,Kaolin组和强力霉素组动物注入37 ℃ 25%Kaolin悬浊液0.6 ml;依同样方法生理盐水组动物仅注入37℃生理盐水0.6 ml;强力霉素组动物给予强力霉素25 mg·kg-1·d-1胃管内注入,持续14 d;假手术组只做枕大池假穿刺术,既不注射Kaolin也不注入生理盐水.在第1、3、7、14天,各时间点随机抽取Kaolin组、强力霉素组、生理盐水组、假手术组动物各6只处死后,取出颈髓,应用干湿法测定脊髓空洞前状态中脊髓含水量,应用免疫印迹和免疫组织化学方法测定脊髓中MMP-9表达含量.结果 各时间点Kaolin组动物颈髓组织中MMP-9表达和脊髓含水量与生理盐水组和假手术组相比明显增高(P<0.01);强力霉素治疗组与Kaolin组相比第1天差异无统计学意义(P>0.05);余各时间点动物脊髓含水量及颈髓组织中MMP-9表达与Kaolin组比较有明显降低(P<0.01).结论 在实验性家兔脊髓空洞症模型中,脊髓MMP-9高表达在脊髓空洞前状态水肿形成中起重要作用,强力霉素具有临床应用治疗脊髓空洞前状态的可能性.%Objective To investigate the relationship between the expression of MMP-9 and the formation of spinal cord edema in presyrinx state of experimental syringomyelia in rabbits and evaluate the inhibitory function of doxycline in the formation of spinal cord edema in presyrinx state. Methods A total of 96 Chinese white rabbits were divided randomly into four groups:kaolin group (n =24), doxycline treatment group (n = 24 ), physiological saline group ( n = 24 ) and control group ( n = 24 ) . Under ketamine

  1. The Iconographical and Pathological Effects of Ozone in Two Concentration on Femoral Artery and Vein in Experimental Rabbits%两种浓度臭氧对实验兔股动脉和股静脉影像学及病理学的影响

    Institute of Scientific and Technical Information of China (English)

    李韶辉; 余革; 陈国勤; 温晓晖

    2014-01-01

    目的:观察两种浓度的臭氧(O3)对实验兔股动脉和股静脉影像及病理学的变化,以评估臭氧对股动脉和股静脉的氧化程度。方法20只实验兔随机分为A、B两组。给予氯胺酮30 mg/kg肌内注射麻醉后,随机选择一侧股动脉和股静脉腹股沟处,在神经刺激器引导下,注射臭氧-氧气混合气体A组浓度50μg/mL 5 mL,B组浓度30μg/mL 5 mL。注射后2 h在实验兔耳缘静脉建立通道,给予氯胺酮10 mg/kg静脉注射麻醉后,再静脉注射碘佛醇2 mL/kg,立即行CT扫描(TOSHIBA AQUILION MULTI)。随后处死实验兔,取注射点腹股沟处股动脉和股静脉2 cm作病理学光镜检查。对侧相应部位作为空白对照。结果与空白对照比较,A、B两组的CT扫描结果显示,注射点腹股沟处股静脉显影正常,未见狭窄或扩张;A、B两组的股静脉直径差异无统计学意义(P>0.05)。50倍和100倍光镜结果显示,注射点腹股沟处股动脉、股静脉结构正常,血管壁完整。结论在股动脉和股静脉旁注射臭氧进行镇痛治疗是安全的。%ObjectiveTo observe the iconographical and pathological changes of femoral artery and vein in experimental rabbits after injection of ozone(O3)in two concentrations.MethodsTwenty experimental rabbits were randomized into either group A (n=10)or Group B (n=10). All rabbits were given intramuscular ketamine 30mg/kg, then mixed O3-O2 5 mL was injected in one randomized side of groin nearby femoral artery and vein under neurostimulator guidance. The concentrations of O3 in Group A and B were 50 μg/mL and 30 μg/mL respectively. Venous access was eatablished through auricular vein 2 h after O3 injection and ketamine 10 mg/kg followed by ioversol 2 mg/kg were given, then CT scan(TOSHIBA AQUILION MULTI) was performed. The rabbits were executed and femoral artery and vein 2 cm in injection site were taken for pathological analysis under light microscope, and

  2. Newer antipsychotics and the rabbit syndrome

    Directory of Open Access Journals (Sweden)

    Masalehdan Azadeh

    2007-06-01

    Full Text Available Abstract Background Rabbit syndrome is a movement disorder that is associated with long-term exposure to neuroleptic medications. Of particular interest and importance is the risk of rabbit syndrome with exposure to the newer atypical antipsychotics. Our recent experience with such a case brought to light the importance of exploring this risk. Methods MEDLINE and PubMed (1972–2006 databases were searched for English language articles using the keywords rabbit syndrome, tardive dyskinesia, antipsychotic, extrapyramidal symptoms and side effects. A recent case study is used to expand upon the literature available on newer antipsychotics and rabbit syndrome. Results We reviewed papers that addressed the following aspects of rabbit syndrome 1 the clinical manifestations 2 prevalence and risk factors, 3 etiopathogenesis 4 older antipsychotics and rabbit syndrome 5 newer antipsychotics, 6 treatment options. Moreover, we report a case of RS in a 50 year old white female, diagnosed with bipolar I disorder, that, after the discontinuation of risperidone, developed involuntary movements of the mouth that were fine, rhythmic and rapid, along the vertical axis, and without involvement of the tongue. After the re-introduction of risperidone, the symptoms decreased in a few hours and disappeared after 3 days. Conclusion Eleven cases of rabbit syndrome have been documented since the implementation of newer antipsychotics. Future research is needed to better understand the etiopathogenesis of rabbit syndrome in psychiatric populations treated with the atypical antipsychotics. Understanding the differences and similarities of rabbit syndrome and tardive dyskinesia is crucial to the creation of a successful treatment paradigm.

  3. Effect of pro biotic Nult-Ox on the growth performance, blood parameters and nutrients digestibility of growing rabbits

    International Nuclear Information System (INIS)

    Fourty growing New Zealand rabbits were used to study the effect of adding commercial Nult-Ox preparation to rabbits drinking water at levels of 0.5 ml/L water once per week (T1), 0.5 ml/L water twice per week (T2), and 0.5 ml/L water three times per week (T3) on growth performance, carcass traits, blood constituents and digestibility coefficients of the nutrients growing New Zealand rabbits. Results revealed that, animals of T3 significantly showed (P<0.05) increases in body weight, weight gain, significant (P<0.05) decreases in feed consumption and feed conversion ratio. Carcass percentage was significantly high in rabbits of T3 followed by rabbits of T2. The blood analysis of the experimental animals showed that, rabbits received Nult-Ox three times per week (T3) showed significant increases in serum total protein, albumin and globulin. Urea, Creatinine, ALT and AST enzymes were significantly lower in T2 and T3, but all the values were within the normal ranges. Digestibility coefficients of DM, OM, CP, EE, CF and NFE of T3 were significantly higher than the other treatments. Data of digestibility coefficiency demonstrated that the addition of Nult-Ox mixture three times per week was effective to upgrade rabbit's performance especially for DM, OM, CP and NFE digestibility more than the other concentrations

  4. Hematologic comparisons of shot and live trapped cottontail rabbits.

    Science.gov (United States)

    Jacobson, H A; Kirkpatrick, R L; Burkhart, H E; Davis, J W

    1978-01-01

    Comparisons were made between hematologic measurements of shot and box-trapped cottontail rabbits (Sylvilagus floridanus). Trapped rabbits had significantly (P less than 0.001) higher serum corticoid levels and segmented neutrophil percentages and significantly (P less than 0.001) lower lymphocyte percentages than did shot rabbits. Trapped rabbits also had significantly (P less than 0.05) higher packed cell volumes and blood urea nitrogen values than did shot rabbits. PMID:633520

  5. Consumer Perceptions of, and Attitudes Toward, Rabbit Meat

    OpenAIRE

    McLean-Meyinsse, Patricia E.; Hui, Jianguo; Meyinsse, Joseph

    1994-01-01

    Results from a consumer-oriented study of households in Louisiana and Texas suggest that the nutritional properties of rabbit meat play a minor role in its consumption. In general, users regard rabbit meat as inferior to chicken, beef or pork and non-users are reluctant to try rabbit meat. Based on these results, the market for rabbit meat is likely to remain small, and rabbit production may not be a viable enterprise for farmers in this region of the country.

  6. A Review of Rabbit Diseases in Egypt

    Directory of Open Access Journals (Sweden)

    Hamed A Mohammed

    2016-02-01

    Full Text Available Promising approaches of the Egyptian governmental as well as non-governmental society to rabbit industry to overcome the unemployment of youth in the society required more efforts from scientific institutes to help in development of such industry. Epidemiological studies are of outmost importance to highlight disease nature and to help in meantime implement of successful preventive and control measures. The aim of this paper is to review the situation of rabbit diseases of economic impact in Egypt (1952 to 2013. The review will highlight the viral infection of rabbit hemorrhagic disease, bacterial disease of colibacillosis, clostridiosis, salmonellosis, pasteurellosis, staphylococcosis and listeriosis and parasitic infection of coccidiosis and mange.

  7. Bobcat attack on a cottontail rabbit

    Science.gov (United States)

    Biggins, D.E.; Biggins, D.M.

    2006-01-01

    We observed an attack by a bobcat (Lynx rufus) on a cottontail rabbit (Sylvilagus) that involved stealthy approach by the cat for >1 h, followed by a 12.3-s chase covering 116.0 m for the cat and 128.4 m for the rabbit. During the chase, the route of the cat from starting point to kill site was more direct than the semi-circular route of the rabbit. Stride lengths for the cat and total distance covered by the chase were longer than those previously reported for bobcats.

  8. Carcass traits of four rabbit genotypes

    OpenAIRE

    Ajda Kermauner; Silvester Zgur

    2010-01-01

    Seventy-three rabbits of four genotypes (A - SIKA maternal line; C - SIKA sire line; AxC - hybrids between line A and C; AxCal - crossbreds between line A and the Californian breed) were used to evaluate the effect of genotype on carcass traits. Rabbits were weaned at 35 days and slaughtered at 93 days of age. Rabbits were fed standard feed mixture ad libitum. The highest live weight at slaughter and dressing percentage was achieved by line C, and the lowest in line A. Hybrids between line A ...

  9. Protective effect of policosanol on atherosclerotic lesions in rabbits with exogenous hypercholesterolemia

    Directory of Open Access Journals (Sweden)

    Arruzazabala M.L.

    2000-01-01

    Full Text Available Policosanol is a mixture of higher aliphatic alcohols purified from sugar cane wax, with cholesterol-lowering effects demonstrable in experimental models and in patients with type II hypercholesterolemia. The protective effects of policosanol on atherosclerotic lesions experimentally induced by lipofundin in rabbits and rats and spontaneously developed in stumptail monkeys have been described. The present study was conducted to determine whether policosanol administered orally to rabbits with exogenous hypercholesterolemia also protects against the development of atherosclerotic lesions. Male New Zealand rabbits weighing 1.5 to 2 kg were randomly divided into three experimental groups which received 25 or 200 mg/kg policosanol (N = 7 orally for 60 days with acacia gum as vehicle or acacia gum alone (control group, N = 9. All animals received a cholesterol-rich diet (0.5% during the entire period. Control animals developed marked hypercholesterolemia, macroscopic lesions and arterial intimal thickening. Intima thickness was significantly less (32.5 ± 7 and 25.4 ± 4 µm in hypercholesterolemic rabbits treated with policosanol than in controls (57.6 ± 9 µm. In most policosanol-treated animals, atherosclerotic lesions were not present, and in others, thickness of fatty streaks had less foam cell layers than in controls. We conclude that policosanol has a protective effect on the atherosclerotic lesions occurring in this experimental model.

  10. Ultrastructural pathological study on skeletal muscle injury in rabbit after a high-dose radiation

    International Nuclear Information System (INIS)

    Objective: To establish a rabbit model of radiation-induced skeletal muscle injury in order to study the ultrastructural pathological changes and underlying mechanism. Methods: 28 New Zealand rabbits were randomly divided into 2 groups with 16 rabbits in experimental group and 12 rabbits in control group. The experimental rabbits were irradiated on hip with a single dose of 80 Gy of 9 MeV electrons from a linear accelerator. 1 month and 6 months after irradiation the pathological changes were respectively observed under light microscope and electron microscope. Results: One month after irradiation, the morphologic changes including degeneration, necrosis of muscle cells, and hemorrhage between the muscle cells were observed under light microscope and the swelling of myofibrillae, blurring of light and shade band, vacuolar degeneration of mitochondria and amorphous areas of necrosis were observed under electron microscope. Six months after irradiation, the morphologic changes of nucleolus chips, fibrous connective tissue, thickening of vascular wall and vascular congestion between the muscle cells and the amorphous areas of necrosis in the experimental group were much more serious than those of 1 month after irradiation. In addition, the myofilaments were lost in degeneration areas and the sarcomere became shorten. Observation with electron microscope showed that the mitochondrial size and its morphological changes were varied and the amounts of collagen between myofibrillaes were increased 6 months after irradiation. Conclusions: A rabbit model of high-dose irradiated skeleton muscle injury was successfully established with a single dose of 80 Gy of 9 MeV electrons from a linear accelerator. The degeneration and necrosis of muscle cells may be promoted by mitochondrial and vascular injury, degeneration of vessel and nerve fiber. (authors)

  11. Detection of acute cerebral hemorrhage in rabbits by magnetic induction

    Energy Technology Data Exchange (ETDEWEB)

    Sun, J.; Jin, G.; Qin, M.X. [College of Biomedical Engineering and Medical Imaging, Third Military Medical University, Chongqing, China, College of Biomedical Engineering and Medical Imaging, Third Military Medical University, Chongqing (China); Wan, Z.B. [Experimental Animal Center, Third Military Medical University, Chongqing, China, Experimental Animal Center, Third Military Medical University, Chongqing (China); Wang, J.B.; Wang, C.; Guo, W.Y. [College of Electronic Engineering, Xidian University, Xi' an, China, College of Electronic Engineering, Xidian University, Xi' an (China); Xu, L.; Ning, X.; Xu, J.; Pu, X.J.; Chen, M.S. [College of Biomedical Engineering and Medical Imaging, Third Military Medical University, Chongqing, China, College of Biomedical Engineering and Medical Imaging, Third Military Medical University, Chongqing (China); Zhao, H.M. [Experimental Animal Center, Third Military Medical University, Chongqing, China, Experimental Animal Center, Third Military Medical University, Chongqing (China)

    2014-02-17

    Acute cerebral hemorrhage (ACH) is an important clinical problem that is often monitored and studied with expensive devices such as computed tomography, magnetic resonance imaging, and positron emission tomography. These devices are not readily available in economically underdeveloped regions of the world, emergency departments, and emergency zones. We have developed a less expensive tool for non-contact monitoring of ACH. The system measures the magnetic induction phase shift (MIPS) between the electromagnetic signals on two coils. ACH was induced in 6 experimental rabbits and edema was induced in 4 control rabbits by stereotactic methods, and their intracranial pressure and heart rate were monitored for 1 h. Signals were continuously monitored for up to 1 h at an exciting frequency of 10.7 MHz. Autologous blood was administered to the experimental group, and saline to the control group (1 to 3 mL) by injection of 1-mL every 5 min. The results showed a significant increase in MIPS as a function of the injection volume, but the heart rate was stable. In the experimental (ACH) group, there was a statistically significant positive correlation of the intracranial pressure and MIPS. The change of MIPS was greater in the ACH group than in the control group. This high-sensitivity system could detect a 1-mL change in blood volume. The MIPS was significantly related to the intracranial pressure. This observation suggests that the method could be valuable for detecting early warning signs in emergency medicine and critical care units.

  12. Detection of acute cerebral hemorrhage in rabbits by magnetic induction

    International Nuclear Information System (INIS)

    Acute cerebral hemorrhage (ACH) is an important clinical problem that is often monitored and studied with expensive devices such as computed tomography, magnetic resonance imaging, and positron emission tomography. These devices are not readily available in economically underdeveloped regions of the world, emergency departments, and emergency zones. We have developed a less expensive tool for non-contact monitoring of ACH. The system measures the magnetic induction phase shift (MIPS) between the electromagnetic signals on two coils. ACH was induced in 6 experimental rabbits and edema was induced in 4 control rabbits by stereotactic methods, and their intracranial pressure and heart rate were monitored for 1 h. Signals were continuously monitored for up to 1 h at an exciting frequency of 10.7 MHz. Autologous blood was administered to the experimental group, and saline to the control group (1 to 3 mL) by injection of 1-mL every 5 min. The results showed a significant increase in MIPS as a function of the injection volume, but the heart rate was stable. In the experimental (ACH) group, there was a statistically significant positive correlation of the intracranial pressure and MIPS. The change of MIPS was greater in the ACH group than in the control group. This high-sensitivity system could detect a 1-mL change in blood volume. The MIPS was significantly related to the intracranial pressure. This observation suggests that the method could be valuable for detecting early warning signs in emergency medicine and critical care units

  13. Axial forces and bending moments in the loaded rabbit tibia in vivo

    Directory of Open Access Journals (Sweden)

    Reifenrath Janin

    2012-03-01

    Full Text Available Abstract Background Different animal models are used as fracture models in orthopaedic research prior to implant use in humans, although biomechanical forces can differ to a great extend between species due to variable anatomic conditions, particularly with regard to the gait. The rabbit is an often used fracture model, but biomechanical data are very rare. The objective of the present study was to measure axial forces, bending moments, and bending axis directly in the rabbit tibia in vivo. The following hypothesis was tested: Axial forces and bending moments in the mid-diaphysis of rabbit tibia differ from other experimental animals or indirectly calculated data. Methods A minifixateur system with 4 force sensors was developed and attached to rabbit tibia (n = 4, which were subsequently ostectomised. Axial forces, bending moments and bending angles were calculated telemetrically during weight bearing in motion between 6 and 42 days post operation. Results Highest single values were 201% body weight [% bw] for axial forces and 409% bw cm for bending moments. Whereas there was a continous decrease in axial forces over time after day 10 (P = 0.03 on day 15, a decrease in bending moments was inconsistent (P = 0.03 on day 27. High values for bending moments were frequently, but not consistently, associated with high values for axial forces. Conclusion Axial forces in rabbit tibia exceeded axial forces in sheep, and differed from indirectly calculated data. The rabbit is an appropriate fracture model because axial loads and bending moments in rabbit tibia were more closely to human conditions than in sheep tibia as an animal model.

  14. Quercetin-induced changes in femoral bone microstructure of adult male rabbits

    Directory of Open Access Journals (Sweden)

    Ramona Babosová

    2016-06-01

    Full Text Available Flavonoids are a group of plant metabolites with antioxidant effects. One of the most abundant flavonoids in the human diet is quercetin. It is found widely in fruits, vegetables and has a lot of beneficial effects on human health. Quercetin has a positive pharmacological effect on bone metabolism and it prevents the organism against bone loss. However, its impact on the size of basic structural units of the compact bone is still unknown. Therefore, the aim of present study was to investigate the impact of the quercetin on femoral bone microstructure in 5-month-old male rabbits. Five rabbits of Californian broiler line were randomly divided into two groups. In the experimental group (E group; n=3, animals were intramuscularly injected with quercetin at dose 1000 μg.kg-1 body weight (bw for 90 days, 3 times per week. Two rabbits without quercetin administration served as a control group (C group. According to our results, intramuscular application of quercetin had an insignificant effect on cortical bone thickness in male rabbits. In these rabbits, changes in qualitative histological characteristics were present in the middle part of the compacta, where primary vascular longitudinal bone tissue was present and expanded there from the periosteum. Also, a lower number of secondary osteons was found in these animals. From the histomorphometrical point of view, significantly decreased sizes of primary osteons' vascular canals and secondary osteons (p <0.05 were found in rabbits administered by quercetin. Our findings indicate that subchronic administration of quercetin at the dose used in our study had considerable impact on both qualitative and quantitative histological characteristics of the compact bone in adult male rabbits.

  15. Determination of tropical forage preferences using two offering methods in rabbits.

    Science.gov (United States)

    Safwat, A M; Sarmiento-Franco, L; Santos-Ricalde, R H; Nieves, D

    2014-04-01

    Two methods of feed preference trials were compared to evaluate the acceptability of 5 fresh foliages: Leucaena leucocephala, Moringa oleifera, Portulaca oleracea, Guazuma ulmifolia, and Brosimum alicastrum that was included as control. The evaluation included chemical analyses and forage intake by rabbits. The first method was a cafeteria trial; 12 California growing rabbits aged 8 wk, allocated in individual cages, were offered the five forage plants at the same time inside the cage, while in the second trial 60 California growing rabbits aged 8 wk, allocated individually, were randomly distributed into 5 experimental groups (n = 12/group); for each group just one forage species was offered at a time. The testing period for each method lasted for 7 d, preceded by one week of adaptation. The results showed that B. alicastrum and L. lecocephala were the most preferred forages while on the contrary G. ulmifolia was the least preferred one by rabbits. The results also revealed that the CV% value for the 2nd method (16.32%), which the tested forages were presented separately to rabbits, was lower and methodologically more acceptable than such value for the 1(st) method (34.28%), which all forages were presented together at the same time. It can be concluded that a range of tropical forages were consumed in acceptable quantities by rabbits, suggesting that diets based on such forages with a concentrate supplement could be used successfully for rabbit production. However, growth performance studies are still needed before recommendations could be made on appropriate ration formulations for commercial use. PMID:25049983

  16. Lymph node mapping in rabbit liver cancer with nanocarbon and methylene blue injecta

    Institute of Scientific and Technical Information of China (English)

    Zhong Li

    2013-01-01

    Objective: To discuss the value of lymph node mapping in rabbit liver cancer with nanocarbon and methylene blue injecta. Methods: Rabbit liver cancer model was established by transplanting VX2 cells with laparotomy in celiac planting method. Twenty Japan white rabbits were divided into two groups randomly. Each group had 10 rabbits. Lymph node mapping in two groups rabbit liver cancer were observed. Two groups rabbit liver cancer and local lymph nodes were removed. The number and location of local lymph nodes were recorded, and then the samples were obtained from both groups. Results: The lymph nodes dyed time was (100.50±29.92) s in nanocarbon group, and (11.20±4.18) s in methylene blue group with statistical significance between two groups (P=0.000). In the comparison of lymph node fading time, nanocarbon group was (2.22±0.74) h, methylene blue group was (1.63±0.54) h, nanocarbon group was longer than the methylene blue group, but without statistical significance (P=0.058). The accuracy was 87.5%(35/40) in methylene blue group, while, the nanocarbon group was 87.2% (34/39) , with statistical significance (P=1.000). Conclusions: Experimental results show that application of nanocarbon injection and methylene blue injection during resection of liver cancer and local lymph nodes in rabbit liver cancer model has obvious tracer function in liver cancer and lymphatic drainage. It can reduce the complexity and risk of the operation, and avoid the blindness in the process of traditional lymph node dissection surgery. Besides, they can effectively reduce the number of residual lymph nodes after operation. It can achieve the lymph node dissection more thoroughly, promptly, easily and safely.

  17. Building of transplanted model of Vx-2 carcinoma in rabbit liver and its DSA features

    International Nuclear Information System (INIS)

    Objective: To establish a stable transplanted model of Vx-2 carcinoma in rabbit from three different transplanted methods, and analyze the DSA (digital subtraction angiography) features of the implanted tumors. Methods: 60 white rabbits of New Zealand were divided into 3 groups at random, 20 for each group. In the first group, Vx-2 carcinoma cells (about 5 x 107) were infused via hepatic artery into the rabbit liver. For the second group, Vx-2 carcinoma cells (about 5 x 107) were implanted into the left lobe of the liver via laparotomic route and in the 3rd group with the same route as the 2nd group, a piece of tumor tissue (contained about 106 - 108 carcinoma cells) were implanted into the left lobe of the liver. And then a comparative observation was performed in 3 groups included: 1. the successful rate of implanted tumors; 2. The volume change and the growth rate of implanted tumors; 3. the biological features of implanted tumors in histopathology and electronic microscope photographs and DSA features of implanted tumors. Results: The successful rates of transplanted tumors in the three groups were 7/20, 10/20 and 19/20 respectively. The successful rate of the 3rd group was the highest of the 3 (P < 0.05) with growth rate as index number. Histopathology and electronic microscope photographs showed that the implanted tumors had infiltrated into the rabbit liver tissue, similar as the biological features of squamous cell carcinoma being transplanted in other sites of rabbits. DSA of the implanted hepatic tumors displayed a hyper-vascular nature. Conclusions: It is successful to establish a rabbit model of Vx-2 liver carcinoma cells transplanted into the rabbit liver with a piece of tumor tissue. The successful rate is obviously higher than the other two methods. The results make possible to gain a reliably mature larger tumor animal model for experimental study on interventional therapy of hepatic carcinoma

  18. Dried chicory root (Cichorium intybus L.) as a natural fructan source in rabbit diet: effects on growth performance, digestion and caecal and carcass traits.

    OpenAIRE

    VOLEK, ZDENEK; Marounek, Milan

    2011-01-01

    Three experimental diets were formulated including (per kg) 100 g oats (control diet), 50 g chicory root (Chicory 5 diet) or 100 g chicory root (Chicory 10 diet) at the expense of oats. The diets differed with respect to starch and fructan contents. In the performance trial, 120 Hyplus rabbits were randomly allocated to three groups (two rabbits per cage) and fed one of the three experimental diets between 31 and 73 days of age. At the end of the trial period, 20 rabbits per treatment were...

  19. Análise comparativa entre esclera tratada com glicerina e esclera tratada com irradiação gama, álcali e glicerina em reconstrução de cavidade anoftálmica: estudo experimental em coelhos Comparative analysis between sclera treated with glycerin and sclera treated with gamma irradiation, alkali and glycerin in the reconstruction of anophthalmic socket: experimental study in rabbits

    Directory of Open Access Journals (Sweden)

    José Byron Vicente Dias Fernandes

    2007-08-01

    reconstruction performed with a polymethylmethacrylate sphere involved in sclera treated with gamma irradiation, alkali and glycerin. The two groups were observed at four moments, at 7, 21, 42 and 84 days after surgery. Preset times elapsed, the rabbits were euthanized, sockets were exenterated, the material was processed for embedment in paraffin, cut into 5 µm sections thick and stained with hematoxylin-eosin and Masson's trichrome techniques. The scleras were submitted to morphometric and semiquantitative analysis. Results were compared by Mann-Whitney's non-parametric test. RESULTS: Comparative analysis of vascularization of the surgical interface pointed out a significative difference in the evaluation done 21 days after the implant (P<0.001, with group II showing greater neovascularization. Comparison of the fibrous capsule thickness displayed a significative difference at moments corresponding to 21 days (P<0.001 and 42 days (P<0.001. At both moments, group II presented a thicker fibrous capsule than group I. Likewise, there was variation in sclera thickness, being thicker in group II, at the 21-day post-implant (P=0.003 moment, and at the 42-day moment (P=0.024, as well. Along the experimental period, when groups were analyzed we could observe statistically significative diminution of vascularization in the surgical interface, in group I, as well as in group II, at the moment of 7, 21, 42 and 84 days. CONCLUSION: When used as coating on the polymethylmethacrylate implant for the reconstruction of rabbit anophthalmic socket, the sclera treated with gamma irradiation, alkali and glycerin reacted similarly to the traditionally used glycerin-treated sclera. The two treatments induced a similar inflammatory response. The formation of neovessels, thickness of fibrous capsule and of sclera developed differently in the two groups. Nevertheless, they presented similar results at the end of the 84-day observation period. There was no significant difference between the two groups

  20. Effect of alpha-tocopherol on bone formation during distraction osteogenesis: a rabbit model

    OpenAIRE

    Kurklu, Mustafa; Yildiz, Cemil; Kose, Ozkan; Yurttas, Yuksel; Karacalioglu, Ozgur; Serdar, Muhittin; Deveci, Salih

    2011-01-01

    Purpose The purpose of this study was to evaluate the effects of alpha-tocopherol on distraction osteogenesis. Materials and methods Right tibias of 30 New Zealand white rabbits were distracted at a rate of 0.5 mm/day for 20 days with a circular external fixator. Experimental group rabbits (n = 15) were administered i.m. 20 mg/kg/day alpha-tocopherol for 30 days. Radiographic examinations were performed at the 20th, 30th and 40th days. Bone scintigraphy was performed at the 5th and 20th days....

  1. Effect of high dose irradiation on the red cell span in rabbits

    International Nuclear Information System (INIS)

    As a part of studies on acute effects of high dose irradiation in vivo, the present report was carried out to evaluate the changes of the red cell life span in the white rabbits by a single whole body exposure to gamma rays from 60Co teletherapy unit. The exposure was done in dose levels of 100, 600 and 900 rads to each experimental group of 10 rabbits. The life span apparent half survival time of red cells, and that the red cell volume in the circulting blood were measured by ICSH Reference method using 51Cr. (Author)

  2. Evaluation of the Association Metformin: Plantago ovata Husk in Diabetic Rabbits

    OpenAIRE

    Raquel Díez-Láiz; García-Vieitez, Juan J.; M. José Diez-Liébana; Matilde Sierra-Vega; Sahagún-Prieto, Ana M.; Calle-Pardo, Ángela P.; Nélida Fernández-Martínez

    2015-01-01

    In this experimental study we have investigated whether the inclusion of the dietary fiber Plantago ovata husk could be recommended as coadjuvant in treatments with oral hypoglycemic drugs. We evaluated the use of Plantago ovata husk-metformin association in diabetic rabbits by determining its effects on glucose and insulin concentrations. Six groups of 6 rabbits were used. Groups 1 to 3 were fed with standard chow and groups 4 to 6 with chow supplemented with Plantago ovata husk (3.5 mg/kg/d...

  3. Development of of macrophage migration inhibition in rabbits infected with virulent Treponema pallidum.

    Science.gov (United States)

    Pavia, C S; Folds, J D; Baseman, J B

    1977-09-01

    Peritoneal exudate cells from rabbits infected with Treponema pallidum Nichols were used as indicators of macrophage migration inhibitory factor activity. Between 3 and 15 weeks after infection, the migration of peritoneal exudate cells was inhibited in the presence of 3 to 25 microgram of T. phagedenis biovar Reiter protein per ml. Before this period, the migration patterns of peritoneal exudate cells from infected animals were uninhibited and similar to those from noninfected control rabbits. These observations were correlated with the development of active cell-mediated immunity during experimental T. pallidum infection. PMID:332632

  4. Estudo experimental comparativo entre o butil-2-cianoacrilato, a mistura gelatina-resorcina-formaldeído e sutura na estabilização de enxertos de cartilagem em coelhos Comparison of butyl-2-cyanocrylate, gelatin-resorcin-formaldehyde (GRF compound and suture in stabilization of cartilage grafts in rabbits

    Directory of Open Access Journals (Sweden)

    Heloisa Juliana Zabeu Rossi Costa

    2006-02-01

    Full Text Available Os enxertos de cartilagem constituem-se em boa opção técnica para aprimoramento das rinoplastias. Procura-se um material para sua fixação que seja de simples manuseio, e que provoque mínima reação tecidual. OBJETIVO: Comparar o uso do butil-2-cianoacrilato, mistura gelatina-resorcina-formaldeído (GRF e sutura na estabilização de enxertos de cartilagem em coelhos. FORMA DE ESTUDO: Experimental. MATERIAL E MÉTODO: Utilizaram-se 15 coelhos adultos da raça Nova Zelândia machos, de cujas orelhas foram ressecados 6 enxertos de cartilagem, fixados no periósteo da calvária e unidos dois a dois com sutura, GRF e cianoacrilato. Após 2, 6 e 12 semanas, grupos de 5 coelhos foram sacrificados e realizou-se avaliação da distância de deslocamento do enxerto e análise histológica do processo inflamatório tecidual e da adesão/deformidade das cartilagens. RESULTADOS: Houve deslocamento menor nas fixadas com GRF do que com cianoacrilato e sutura. O processo inflamatório foi maior nos animais de 2 semanas, decaindo até restar pequeno grau de fibrose em 12 semanas, de modo semelhante nas 3 fixações. Não houve descolamento nem deformidade em nenhuma dupla de cartilagens fixada com sutura e o maior número de cartilagens descoladas e deformadas se deu com o cianoacrilato. O número de cartilagens deformadas foi diretamente proporcional ao número de descoladas. Os dados foram significativos estatisticamente (pCartilage grafting is an interesting option for rinoplasties refinements. AIM: to compare butyl-2-cyanocrylate, compound gelatin-resorcin-formaldeyide (GRF and suture control to determine the efficacy of these tissue glue preparations in securing grafted cartilage. STUDY DESIGN: Experimental. METHODS: Fifteen male adult New Zealand rabbits were submitted to a surgical procedure to harvest 6 auricular cartilage grafts from each animal. 2 of these grafts in each animal were glued together with butyl-2-cyanocrylate, 2 with compound

  5. Carcass traits of four rabbit genotypes

    Directory of Open Access Journals (Sweden)

    Ajda Kermauner

    2010-01-01

    Full Text Available Seventy-three rabbits of four genotypes (A - SIKA maternal line; C - SIKA sire line; AxC - hybrids between line A and C; AxCal - crossbreds between line A and the Californian breed were used to evaluate the effect of genotype on carcass traits. Rabbits were weaned at 35 days and slaughtered at 93 days of age. Rabbits were fed standard feed mixture ad libitum. The highest live weight at slaughter and dressing percentage was achieved by line C, and the lowest in line A. Hybrids between line A and C exhibited slightly worse carcass traits than rabbits in line C, but the differences were not statistically significant. The Californian breed gave worse results than crossbreeding with line C, though in most cases the differences between AxC and AxCal were not significant. The differences between genotypes in hind leg tissue composition, pH and meat colour were not statistically significant.

  6. Analysis of immune responses against H pylori in rabbits

    Institute of Scientific and Technical Information of China (English)

    Khademul Islam; Ibrahim Khalil; Chowdhury Rafiqul Ahsan; Mahmuda Yasmin; Jamalun Nessa

    2007-01-01

    AIM: To investigate the immunogenicity of H pylori proteins, to evaluate the production rate of anti H pylori IgG antibodies in relation to time and to demonstrate the fidelity of newly optimized in-house enzymelinked immunosorbent assay (ELISA) technique as an alternative for H pylori infection assay.METHODS: In the present study, 100 μg of formalinfixed H pylori whole cell antigens was injected into an experimental animal (New Zealand white female rabbit) intramuscularly on d 0, 16, 27 and 36. The first two doses were injected with adjuvants. On d 0,a serum sample was collected from the rabbit before immunization and this pre-immunized serum was used as a negative control for the whole study. To evaluate the immunogenic responses of the injected antigen,serum samples were collected from the rabbit at regular intervals up to d 42. The sera were analyzed using inhouse ELISA and Western blot techniques.RESULTS: The production of anti H pylori IgG antibodies in the rabbit in response to the injected antigen increased almost exponentially up to d 14 and after that it was maintained at the same level until the last day (d 42). By analyzing the immune profiles of immunized sera, 11 proteins were identified to be immunogenic,among them 2 (approximately 100 kDa and 85 kDa)were most prominent.CONCLUSION: Analysis of the immune responses against pathogenic microorganisms like H pylori is necessary for the development of various diagnostic and preventive approaches. The results of this experiment reveal that the formalin-fixed H pylori whole cell antigens injected into the rabbit are highly immunogenic. These prominent proteins (approximately 100 kDa and 85 kDa)might have higher immunogenic effects among humans infected with H pylori and some of these immunogenic proteins can be included in diagnostic approaches based on serology and also for vaccine formulation. The inhouse ELISA is a promising alternative compared to invasive techniques.

  7. Phototoxic effects of 8-methoxypsoralen on rabbit corneal endothelium

    International Nuclear Information System (INIS)

    The phototoxic effects of 8-methoxypsoralen (8-MOP) were investigated using the rabbit corneal endothelium in organ culture. The corneas were divided into four groups: (a) irradiated with a mercury vapor lamp (emitting UVA and visible radiation) in the presence of 8-MOP (experimental), (b) irradiated without 8-MOP (control A), (c) incubated with 8-MOP (control B) and (d) incubated without 8-MOP (control C). Specular and light microscopic examination showed that the experimental corneas had greater cellular damage compared to the control corneas. The effects of 8-MOP were restricted to certain localized areas of the cornea. However there was no significant difference in the amounts of 51Cr released from the labelled experimental and control corneas. These results show phototoxic damage of the corneal endothelial cells

  8. Phototoxic effects of 8-methoxypsoralen on rabbit corneal endothelium

    Energy Technology Data Exchange (ETDEWEB)

    Menon, I.A.; Basu, P.K.; Hasany, S.M.; Persad, S.D. (Univ. of Toronto, Ontario (Canada))

    1989-01-01

    The phototoxic effects of 8-methoxypsoralen (8-MOP) were investigated using the rabbit corneal endothelium in organ culture. The corneas were divided into four groups: (a) irradiated with a mercury vapor lamp (emitting UVA and visible radiation) in the presence of 8-MOP (experimental), (b) irradiated without 8-MOP (control A), (c) incubated with 8-MOP (control B) and (d) incubated without 8-MOP (control C). Specular and light microscopic examination showed that the experimental corneas had greater cellular damage compared to the control corneas. The effects of 8-MOP were restricted to certain localized areas of the cornea. However there was no significant difference in the amounts of 51Cr released from the labelled experimental and control corneas. These results show phototoxic damage of the corneal endothelial cells.

  9. Parasitic infections of wild rabbits and hares

    OpenAIRE

    Ilić Tamara; Petrović Tamaš; Dimitrijević Sanda

    2014-01-01

    The paper presents the most important parasitic infections of wild rabbits and hares, which harmful effect in this animal population is manifested as a gradual weakening of the immune system, reduction in fertility, weight loss and constant exhaustion. Order of Lagomorpha (hares or lagomorphs) belongs to superorder of higher mammals which includes the family of rabbits (Leporidae) which are represented in Europe as well as the family of whistleblowers (Ocho...

  10. Learning about the rabbit in kindergarten

    OpenAIRE

    Roblek, Tadeja

    2012-01-01

    The present diploma thesis presents the biology of hares and rabbits and key differences between them. It describes the importance of living creatures for the child's development, the concept of learning by experience and the importance of children's books for the child's development. The purpose of the diploma thesis is to analyse the accuracy of information in children's books that present hares and rabbits. The thesis aims to explore the importance of learning by experience in pre-scho...

  11. Effect of Drugs on Rabbit Retina

    OpenAIRE

    Cardiakidis Myers, Anna

    2013-01-01

    The adverse effects of drugs in the eye are not easily detected, and the underlying pathogenesis not fully understood. The effects of four different kinds of drugs have been investigated in the rabbit eye, using electroretinography, ocular examination and histochemical methods. Rifabutin is a broad spectrum antibiotic with known ocular adverse effects (corneal opacities, uveitis and vascular retinopathy) in the human eye. In the rabbit eye rifabutin causes lens discoloration and it si...

  12. Ultrastructural study of developing rabbit diaphragm.

    OpenAIRE

    Yamashiro, S; Harris, W. H.; Stopps, T.P.

    1984-01-01

    Diaphragms obtained from forty rabbit fetuses during the last third of gestation and from five rabbits one week old were studied ultrastructurally. Four gestational ages were chosen for the investigation. The diaphragm at 20 days gestation revealed early myogenesis which was characterised by many myogenic cells in the process of mitosis and by fusion of myoblasts to form myotubes. A large number of lipid droplets and a moderate quantity of glycogen were observed in the myotubes. These myotube...

  13. Rabbit cationic protein enhances leukocyte adhesiveness.

    OpenAIRE

    Oseas, R S; Allen, J; Yang, H. H.; Baehner, R. L.; Boxer, L A

    1981-01-01

    Cationic protein purified from rabbit peritoneal polymorphonuclear leukocytes (PMN) was demonstrated to incite autoaggregation of the rabbit PMN and promote adhesiveness of human PMN to endothelial cells. PMN aggregation induced by supernatants derived from secretory PMN was blocked by a specific anticationic protein antibody. These studies reveal that a positively charged protein derived from the PMN can alter surface properties of the PMN itself and imply a role for this protein in PMN immo...

  14. A Review of Rabbit Diseases in Egypt

    OpenAIRE

    Hamed A Mohammed; Eid AA M; El-Bakrey R MM

    2016-01-01

    Promising approaches of the Egyptian governmental as well as non-governmental society to rabbit industry to overcome the unemployment of youth in the society required more efforts from scientific institutes to help in development of such industry. Epidemiological studies are of outmost importance to highlight disease nature and to help in meantime implement of successful preventive and control measures. The aim of this paper is to review the situation of rabbit diseases of economic impact in ...

  15. Organotypic Culture of Adult Rabbit Retina

    OpenAIRE

    Lye, Ming H.; Jakobs, Tatjana C.; Masland, Richard H.; Koizumi, Amane

    2007-01-01

    Organotypic culture systems of functional neural tissues are important tools in neurobiological research. Ideally, such a system should be compatible with imaging techniques, genetic manipulation, and electrophysiological recording. Here we present a simple interphase tissue culture system for adult rabbit retina that requires no specialized equipment and very little maintenance. We demonstrate the dissection and incubation of rabbit retina and particle-mediated gene transfer of plasmids enco...

  16. Training in rabbit husbandry in Mozambique

    OpenAIRE

    Facchin, E.; Gallazzi, D.; Gaspari, D.; PARIGI-BINI, R.

    1989-01-01

    The possibility of the meat rabbit production in developing countries with reference to Mozambique situation is treated. The rabbit husbandry in Mozambique is feasible but the cultural passage from the passive rearing habits to a modem intensive technological rearing it will be neither easy nor next to corne. Three kinds of husbandry could be possible in Mozambique : a) intensive b) complementary and c) family husbandry ; a) can be made only in well organized farm and the limits for intensive...

  17. Reproductive activity and welfare of rabbit does

    OpenAIRE

    Castellini, C.

    2010-01-01

    This paper reviews the relationships between reproductive performance and welfare of the rabbit does. In the last 10 years the profitability of rabbit farms has increased mainly due to improvements in management and genetic selection but several problems mainly related to animal welfare have also occurred. The mortality and rates of female replacement per year are very high and the replaced females often show poor body condition and low performance. The effect of kindling order, litter size, ...

  18. Establishment of a new model for culturing rabbit osteoblasts in vitro

    International Nuclear Information System (INIS)

    To establish an experimental model for culturing rabbit osteoblasts in vitro, the osteoblasts were isolated from the calvarial bone of a 15-day old rabbit using a method of culturing the bone pieces in a medium after they had been digested by an enzyme for 15 min. The acquired cells were assayed by cell morphology, alkaline phosphatase activity and production of a mineralized matrix. The results showed that the cells had the morphologic characteristics and some biological behaviours of osteoblasts. Based on the primary isolation of osteoblasts from bone and combining digestion with explants, a novel model for culturing rabbit osteoblasts in vitro was established, which is easy, efficient and effective. This model can be used in many studies of osteogenesis mechanisms and bone replacement materials. (communication)

  19. Levan-induced glomerulitis in rabbits: a possible role for direct complement activation in situ.

    Science.gov (United States)

    Stark, H.; Alkalay, A.; Ben-Bassat, M.; Hazaz, B.; Joshua, H.

    1985-01-01

    Since high-molecular-weight levan is known to reduce capillary permeability to large molecules, an experiment was designed to investigate whether this agent may attenuate the glomerulonephritis associated with acute serum sickness in rabbits. The study, in fact, demonstrated an enhancing effect of levan, which caused increased glomerular proliferative changes and leucocyte infiltration and, possibly, increased IgG deposition in this experimental model. In addition, rabbits injected only with levan also demonstrated mild glomerulitis and C3 deposition. In one of four rabbits examined, this was accompanied by a marked fall in the serum level of total haemolysing complement. Levan was demonstrated to cause activation of complement when incubated with normal rabbit serum in vitro. We believe that these findings are best explained on the basis of complement activation in situ by levan in the glomeruli, probably via the alternative pathway, with the resulting inflammatory response. In the case of BSA-injected rabbits, this response is believed to be additive to that of the classical immune complex-mediated complement activation. Images Fig. 1 PMID:3986130

  20. Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits

    Directory of Open Access Journals (Sweden)

    CN Zanuzzi

    2009-08-01

    Full Text Available The intestinal epithelium has a critical roll in host defence. One specialised cell type involved in this function is the Paneth cell, which secretes many substances with antimicrobial properties in response to different stimuli. Under pathological conditions, changes in the Paneth cell number, morphology and location as well as in granule number, morphology and composition have been reported. In the normal animal, 1,25-dihydroxyvitamin D3 participates in the maintenance of mineral homeostasis, immunomodulation and cell proliferation and differentiation. Solanum glaucophyllum, a calcinogenic plant containing high levels of 1,25-dihydroxyvitamin D3, is responsible for a condition known as enzootic calcinosis in ruminants, characterised by loss of body condition and mineralization of soft tissues. Using and established rabbit model, this study analyses the changes that rabbit Paneth cells undergo during intoxication with S. glaucophyllum. Male New Zealand white rabbits were experimentally intoxicated with S. glaucophyllum for 15 or 30 days. Lectin, immunohistochemical and morphometric studies were carried out on Paneth cells from samples of jejunum. SBA, DBA and WGA lectins bound to Paneth cellsgranules in both normal and intoxicated rabbits, with more heterogenity in the labelling of granules from intoxicated rabbits. Paneth cells in both groups were immunonegative for lysosyme. A time and dose-dependent increase in the size and number of Paneth cells was found in both intoxicated groups.We suggest that the changes described in these cells may be directly or indirectly induced by S. glaucophyllum intoxication.

  1. Marsh rabbit mortalities tie pythons to the precipitous decline of mammals in the Everglades

    Science.gov (United States)

    McCleery, Robert A.; Sovie, Adia; Reed, Robert N.; Cunningham, Mark W.; Hunter, Margaret E.; Hart, Kristen M.

    2015-01-01

    To address the ongoing debate over the impact of invasive species on native terrestrial wildlife, we conducted a large-scale experiment to test the hypothesis that invasive Burmese pythons (Python molurus bivittatus) were a cause of the precipitous decline of mammals in Everglades National Park (ENP). Evidence linking pythons to mammal declines has been indirect and there are reasons to question whether pythons, or any predator, could have caused the precipitous declines seen across a range of mammalian functional groups. Experimentally manipulating marsh rabbits, we found that pythons accounted for 77% of rabbit mortalities within 11 months of their translocation to ENP and that python predation appeared to preclude the persistence of rabbit populations in ENP. On control sites, outside of the park, no rabbits were killed by pythons and 71% of attributable marsh rabbit mortalities were classified as mammal predations. Burmese pythons pose a serious threat to the faunal communities and ecological functioning of the Greater Everglades Ecosystem, which will probably spread as python populations expand their range.

  2. Marsh rabbit mortalities tie pythons to the precipitous decline of mammals in the Everglades.

    Science.gov (United States)

    McCleery, Robert A; Sovie, Adia; Reed, Robert N; Cunningham, Mark W; Hunter, Margaret E; Hart, Kristen M

    2015-04-22

    To address the ongoing debate over the impact of invasive species on native terrestrial wildlife, we conducted a large-scale experiment to test the hypothesis that invasive Burmese pythons (Python molurus bivittatus) were a cause of the precipitous decline of mammals in Everglades National Park (ENP). Evidence linking pythons to mammal declines has been indirect and there are reasons to question whether pythons, or any predator, could have caused the precipitous declines seen across a range of mammalian functional groups. Experimentally manipulating marsh rabbits, we found that pythons accounted for 77% of rabbit mortalities within 11 months of their translocation to ENP and that python predation appeared to preclude the persistence of rabbit populations in ENP. On control sites, outside of the park, no rabbits were killed by pythons and 71% of attributable marsh rabbit mortalities were classified as mammal predations. Burmese pythons pose a serious threat to the faunal communities and ecological functioning of the Greater Everglades Ecosystem, which will probably spread as python populations expand their range. PMID:25788598

  3. Computer tomographic imaging of rabbit bulbourethral glands

    International Nuclear Information System (INIS)

    The aim of the study was to utilize the obtained data for differentiation of normal and pathologically altered bulbourethral glands in rabbits with regard to using this animal species as a model for studying diseases in this organ in humans. MATERIAL AND METHODS: Ten sexually mature healthy male white New Zealand rabbits, 12 months old, weighed 2.8−3.2 kg were investigated. The animals were anesthetized. Scans were done at 2 mm intervals and the image reconstruction was three-dimensional. RESULTS: Rabbit bulbourethral glands were observed as a transversely oval homogeneous, relatively hyperdense structure against the surrounding soft tissues. They are visualized in the transverse cut of the pelvic outlet in the plane through the cranial part of cg2, the body of ischium, cranially to tuber ischiadicum and dorsally to the caudal part of symphysis pubis –sciatic arch. The glandular margins are adequately distinguished from the adjacent soft tissue structures. The density of the rabbit bulbourethral glands was similar to this of the soft tissues. CONCLUSION: The data obtained by the computed tomographic imaging of the rabbit bulbourethral glands could be used as an anatomical reference in the diagnosis and interpretation of imaging findings of various pathological states of the gland in this species, as well as in utilization of the rabbit as an animal model for studying diseases of this organ in humans, particularly diverticula, stenosis, lithiasis and valves

  4. Genomic Analysis of Companion Rabbit Staphylococcus aureus

    Science.gov (United States)

    Holmes, Mark A.; Harrison, Ewan M.; Fisher, Elizabeth A.; Graham, Elizabeth M.; Parkhill, Julian; Foster, Geoffrey; Paterson, Gavin K.

    2016-01-01

    In addition to being an important human pathogen, Staphylococcus aureus is able to cause a variety of infections in numerous other host species. While the S. aureus strains causing infection in several of these hosts have been well characterised, this is not the case for companion rabbits (Oryctolagus cuniculus), where little data are available on S. aureus strains from this host. To address this deficiency we have performed antimicrobial susceptibility testing and genome sequencing on a collection of S. aureus isolates from companion rabbits. The findings show a diverse S. aureus population is able to cause infection in this host, and while antimicrobial resistance was uncommon, the isolates possess a range of known and putative virulence factors consistent with a diverse clinical presentation in companion rabbits including severe abscesses. We additionally show that companion rabbit isolates carry polymorphisms within dltB as described as underlying host-adaption of S. aureus to farmed rabbits. The availability of S. aureus genome sequences from companion rabbits provides an important aid to understanding the pathogenesis of disease in this host and in the clinical management and surveillance of these infections. PMID:26963381

  5. Light colour preference of growing rabbits

    Directory of Open Access Journals (Sweden)

    Zsolt Szendrő

    2010-01-01

    Full Text Available The objective of the experiment was to evaluate the light colour preference of growing rabbits placed in a free-choice cage. The experiment was carried out on 128 Pannon White growing rabbits weaned at the age of 5 weeks and placed into blocks (2m2 of four cages. The rabbits could move freely among the four cages (0.5m2 each through swing doors. The cages differed only in the colour of the light applied (white, yellow, green or blue. The lighting schedule was 16L: 8D. From 6 until 10 weeks of age, infrared video recording was performed once a week (24 hours. The number of rabbits in each cage was counted every 15 minutes. Feed consumption was measured weekly. Between 6 and 10 weeks of age the rabbits significantly preferred white light (28.0%. The preference order was the following: yellow (26.3%, blue (23.4% and green (22.3% (P<0.001. No significant differences were recorded in the feed consumption among the cages. In conclusion, the cage preference of the rabbits was slightly affected by the light colour.

  6. The possibility of using irradiated untraditional feed-stuffs in rabbits nutrition

    International Nuclear Information System (INIS)

    The present work was designed and carried out at food irradiation research department , National Center for Radiation Research and Technology (NCRRT), Nasr City, Cairo , Egypt , in co-operation with animal production department, faculty of agriculture, Moshtohor, Benha University. The main target of the present study was to evaluate, improve and upgrade the utilization of olive cake and the possibility of replacing part of (clover hay) in rabbits diets by either non-or irradiated processed olive cake with gamma irradiation at dose levels of 20 and 60 kGy . The performance of growing rabbits digestibility, nutritive values of experimental diets, alongside some blood haematological and biochemical parameters, violability rate and economical efficiency were also studied. The growing rabbits were fed on diets without olive cake by - product (control diet) , or with 15% raw or processed olive cake at the above mentioned radiation dose (20 and 60 kGy) from cobalt-60 radioisotopes. Accordingly, a total of 4 experimental diets were used. The experimental diets were formulated to be iso-nitrogenous ( ∼ 17% CP), iso - caloric (∼2500 kcal DE/kg diet) and to , satisfy the nutrients requirements of growing rabbits according to agriculture ministry decree (1996)

  7. Refining Operational Practice for Controlling Introduced European Rabbits on Agricultural Lands in New Zealand

    Science.gov (United States)

    Latham, A. David M.; Latham, M. Cecilia; Nugent, Graham; Smith, James; Warburton, Bruce

    2016-01-01

    European rabbits (Oryctolagus cuniculus) pose a major threat to agricultural production and conservation values in several countries. In New Zealand, population control via poisoning is a frontline method for limiting rabbit damage, with large areas commonly treated using the metabolic toxin sodium fluoroacetate (‘1080’) delivered in bait via aerial dispersal. However, this method is expensive and the high application rates of the active ingredient cause public antipathy towards it. To guide reductions in cost and toxin usage, we evaluated the economics and efficacy of rabbit control using an experimental approach of sowing 1080-bait in strips instead of the commonly-used broadcast sowing method (i.e. complete coverage). Over a 4-year period we studied aerial delivery of 0.02% 1080 on diced carrot bait over ~3500 ha of rabbit-prone land in the North and South islands. In each case, experimental sowing via strip patterns using 10–15 kg of bait per hectare was compared with the current best practice of aerial broadcast sowing at 30–35 kg/ha. Operational kill rates exceeded 87% in all but one case and averaged 93–94% across a total of 19 treatment replicates under comparable conditions; there was no statistical difference in overall efficacy observed between the two sowing methods. We project that strip-sowing could reduce by two thirds the amount of active 1080 applied per hectare in aerial control operations against rabbits, both reducing the non-target poisoning risk and promoting cost savings to farming operations. These results indicate that, similarly to the recently-highlighted benefits of adopting strip-sowing for poison control of introduced brushtail possums (Trichosurus vulpecula) in New Zealand, aerial strip-sowing of toxic bait could also be considered a best practice method for rabbit control in pest control policy. PMID:27341209

  8. Adjunctive Phosphodiesterase-4 Inhibitor Therapy Improves Antibiotic Response to Pulmonary Tuberculosis in a Rabbit Model

    Science.gov (United States)

    Subbian, Selvakumar; Tsenova, Liana; Holloway, Jennifer; Peixoto, Blas; O'Brien, Paul; Dartois, Véronique; Khetani, Vikram; Zeldis, Jerome B.; Kaplan, Gilla

    2016-01-01

    Objectives Adjunctive host-directed therapy is emerging as a new potential approach to improve the outcome of conventional antimicrobial treatment for tuberculosis (TB). We tested the ability of a phosphodiesterase-4 inhibitor (PDE4i) CC-11050, co-administered with the first-line anti-TB drug isoniazid (INH), to accelerate bacillary killing and reduce chronic inflammation in the lungs of rabbits with experimental Mycobacterium tuberculosis (Mtb) infection. Methods A rabbit model of pulmonary TB that recapitulates the pathologic manifestations seen in humans was used. Rabbits were infected with virulent Mtb by aerosol exposure and treated for eight weeks with INH with or without CC-11050, starting at four weeks post infection. The effect of CC-11050 treatment on disease severity, pathology, bacillary load, T cell proliferation and global lung transcriptome profiles were analyzed. Results Significant improvement in bacillary clearance and reduced lung pathology and fibrosis were noted in the rabbits treated for eight weeks with INH + CC-11050, compared to those treated with INH or CC-11050 only. In addition, expression of host genes associated with tissue remodeling, tumor necrosis factor alpha (TNF-α) regulation, macrophage activation and lung inflammation networks was dampened in CC-11050-treated, compared to the untreated rabbits. Conclusions Adjunctive CC-11050 therapy significantly improves the response of rabbits with experimental pulmonary TB to INH treatment. We propose that CC-11050 may be a promising candidate for host directed therapy of patients with pulmonary TB, reducing the duration and improving clinical outcome of antibiotic treatment. PMID:26981575

  9. Evaluation of clinical and paraclinical effects of intraosseous vs intravenous administration of propofol on general anesthesia in rabbits

    Directory of Open Access Journals (Sweden)

    Ramin Mazaheri-Khameneh

    2012-06-01

    Full Text Available This prospective study aimed to compare the intraosseous (IO and intravenous (IV effects of propofol on selected blood parameters and physiological variables during general anesthesia in rabbits. Thirty New Zealand White rabbits were studied. Six rabbits received IV propofol (group 1 and another 6 rabbits, were injected propofol intraosseously (Group 2 for 30 minutes (experimental groups. Rabbits of the third and fourth groups received IV and IO normal saline at the same volume given to the experimental groups, respectively. In the fifth group IO cannulation was performed but neither propofol nor normal saline were administered. Blood profiles were assayed before induction and after recovery of anesthesia. Heart and respiratory rates, rectal temperature, saturation of peripheral oxygen and mean arterial blood pressure were recorded. Heart rate increased significantly 1 to 5 minutes after induction of anesthesia in experimental groups (P < 0.05. Although mean arterial blood pressure decreased significantly from baseline, values remained above 60 mm Hg (P < 0.05. Respiratory rate decreased significantly in experimental groups, but remained higher in group 2 (P < 0.05. The lymphocyte count decreased significantly in group 1 (P < 0.05. The concentration of alkaline phosphatase in all rabbits, aspartate aminotransferase and gamma- glutamyl transferase in the first group and gamma-glutamyl transferase in the third group increased significantly (P < 0.05. Total bilirubin decreased significantly in group 2 (P < 0.05. All measured values remained within normal limits. Based on the least significant physiological, hematological and biochemical effects, the IO injection of propofol appears to be safe and suitable method of anesthesia in rabbits with limited vascular access.

  10. Serologic evidence for rabbit syncytium virus in eastern cottontail rabbits (Sylvilagus floridanus) in Ohio.

    Science.gov (United States)

    Theil, K W; McCloskey, C M; Scott, D P

    1993-07-01

    Thirteen of 20 eastern cottontail rabbit (Sylvilagus floridanus) sera collected near Delaware, Ohio (USA) in 1991 were positive by indirect immunofluorescent antibody test (IFAT) for antibody to rabbit syncytium virus (RSV), a Kemerovo serogroup orbivirus. In addition, two of 10 domestic bovine sera and three of 30 sheep sera collected in southeastern Ohio gave weak positive IFAT reactions to RSV. PMID:8394944

  11. NF-KB p65及环氧化酶-2在实验性变态反应性脑脊髓炎中的表达及其作用机制%Expression of nuclear factor-kB and COX-2 and their role in experimental allergic encephalomyelitis in rabbits

    Institute of Scientific and Technical Information of China (English)

    高聪; 谢富华; 殷建瑞; 杨洁; 冯欣; 马鹏程

    2008-01-01

    Objective To study the expression of nuclear factor-kB (NF-KB) and COX-2 and their relationship in experimental allergic encephalomyelitis (EAE), and investigate their role in the pathogenesis of EAE. Methods All rabbits were classified three groups in according to making models by different immunogens: group A is immuned without immunogen, group B is immuned with R-SCH, group C is immunod with C-SCH. Immunofluorescence and immunohistochemistry were performed for morphological observations of the brain tissues in all animals, and then the activity of NF-KBp65 was detected using enzyme-linked immunosorbent assay. Results The expressions of NF-KBp65 and COX-2 were observed in brain issues with EAE. Significant differences were found in the number of cells positive for NF-KBp65 and COX-2 between EAE groups and the control group. NF-KBp65 and COX-2 expressions were associated with the symptoms of EAE. In addition, significantly increased activity ofNF-kBp65 was noted in EAE, which is immuned with R-SCH. Conclusion NF-KB and COX-2 are expressed in rabbits with EAE and play important roles in the pathogenesis of EAE.%目的 研究核转录因子NF-kB p65及环氧化酶-2(COX-2)在实验性变态反应性脑脊髓炎(EAE)中的表达,探讨其参与EAE的作用机制. 方法 健康新西兰兔30只根据采用不同免疫原制作模型的方式分为三组:A组,正常对照组(未注射免疫原)10只;B组,新西兰兔脊髓匀浆(R-SCH)EAE模型组,10只;C组,牛脑匀浆(C-SCH)EAE模型组,10只.比较每组动物临床病理特点;分别采用免疫荧光、免疫组化对其进行形态观察,应用ELISA法对NF-KBp65进行活性测定.结果 B、C组均可见NF-kB p65及COX-2明显增加,阳性细胞百分率与A组比较.差异有统计学意义(P<0.05);其中B组NF-kB p65活性明显增高.结论 在EAE模型中存在NF-kBp65及COX-2表达,它们均在EAE发病过程中发挥了重要作用.

  12. Phosphatidylinositol kinase from rabbit reticulocytes

    International Nuclear Information System (INIS)

    Phosphatidylinositol (PI) kinase was isolated from the postribosomal supernatant of rabbit reticulocytes. This activity was identified by the formation of a product that comigrated with phosphatidylinositol-4-phosphate (PIP) when purified PI was phosphorylated in the presence of [32P]ATP and Mg2+. Three major peaks of PI kinase activity were resolved by chromatography on DEAE-cellulose. The first peak eluted at 50-100 mM NaCl together with several serine protein kinases, casein kinase (CK) I and protease activated kinase (PAK) I and II. The PI kinase was subsequently separated from the protein kinases by chromatography on phosphocellulose. The second peak eluted at 125-160 mM NaCl and contained another lipid kinase activity that produced a product which comigrated with phosphatidic acid on thin layer chromatography. The third peak, which eluted at 165-200 mM NaCl, partly comigrated with casein kinase (CK) II and an active protein kinase(s) which phosphorylated mixed histone and histone I. CK II and the histone kinase activities were also separated by chromatography on phosphocelluslose. The different forms of PI kinase were characterized and compared with respect to substrate and salt requirements

  13. CURRENT STATUS, CHALLENGES AND OPPORTUNITIES OF RABBIT PRODUCTION IN BOTSWANA

    Directory of Open Access Journals (Sweden)

    J.C. MOREKI

    2012-03-01

    Full Text Available This review highlights the current status of rabbit production, challenges facing the industry and opportunities available. Rabbit farming in Botswana is in its infancy and the rabbit population is estimated to be less than 1000. However, this value is a gross underestimate due to poor monitoring by government extension services. In Botswana, rabbits are mainly kept in the backyards, indicating that intensive systems have not yet been developed. Rabbits have small body size, short gestation period, high reproductive potential, rapid growth rate and ability to utilize forages. Compared to beef, chicken, mutton, chevon and chicken, rabbit meat has low cholesterol, high protein and low fat contents. Rabbit production can be integrated into small farming systems, with the rabbits being fed on crop residues, weeds, poultry droppings, and kitchen and garden wastes. The manure can be used to fertilize soils. The major challenges in rabbit production are inadequacy of breeding stock, inadequate rabbit feeds, poor management (feeding, housing and health care, lack of research support, lack of technical support from extension services, lack of access to credit and inadequate supply of equipment. The major opportunity available to the rearers is that the market is vast due to the small rabbit population in the country. The attributes of rabbits suggest that rabbit farming is likely to play an important role in nutrition, poverty alleviation and food security, especially in countries with higher unemployment levels and HIV/AIDS prevalence rates such as Botswana.

  14. 高脂饲料加空气干燥术建立兔颈动脉粥样硬化模型%A Novel Experimental Carotid Atherosclerotic Rabbit Model Induced by High-Chole sterol-Diet Plus Air-Drying

    Institute of Scientific and Technical Information of China (English)

    张磊; 陈国荣; 郑荣远; 王群姬; 谢淑萍; 王拥军

    2001-01-01

    为建立一种新的比较适合于研究颈动脉粥样硬化的动物模型,将54 只日本大耳白兔分为3组,分别给予高脂饲料喂养加空气干燥术(n=24)、单纯高脂饲料喂养(n=24)和正常饲料喂养(n=6)。在实施空气干燥术后的第3天、第1周、第2周和第4周时分别处死动物,观察颈动脉病变的形态特征,计量其内膜增厚程度。结果发现,在高脂饲料加空气干燥术组出现较典型的动脉粥样硬化病变,包括内皮细胞再生、内膜增厚、平滑肌细胞移行增殖、脂质沉积、弹力纤维和胶原基质的生成等。第2周和第4周时内膜增生明显,内膜与中膜面积比值分别达到0.47±0.07和0.94±0.07,而在单纯高脂饲料组的颈动脉和高脂饲料加空气干燥术组的对侧颈动脉未发现明显的动脉粥样硬化病变。以上结果提示,高脂饲料加空气干燥术可以使家兔形成典型的颈动脉粥样硬化病变,其病理改变较适合于研究颈动脉粥样硬化。%Aim To establish a new animal model which is more suitable for the study on carotid atherosclerosis and to investigate its mechanisms. Methods 54 male Japanese White rabbits were randomly divided into 3 groups. The first group was fed by normal diet as blank control (n =6), the second group was fed by high-cholesterol diet as choleste r ol-fed control groups (n=24), the third group was fed by high-cholesterol diet and treated by air-drying as experimental groups ([ W TBX n=24). Animals were sacrificed at various times (from 3 day s to 4 weeks) after surgery in the experimental groups and at the corresponding times in the control groups. The carotid arteries were prepared for examination by light microsocopy and by transmission electron microsocopy. The intimal thickening were evaluated by intimal area/medial area ratio(I/M ra tio). Results The typical carotid atherosclerosis was observ ed in the experimental groups

  15. Delineation of Experimental ECG Records

    OpenAIRE

    Bucsuházy, K.

    2015-01-01

    This work deals with QRS complex detection and ECG delineation. The proposed method is based on redundant dyadic discrete wavelet transform. It is designed for experimental electrocardiograms of isolated rabbit hearts and it is evaluated through manually determined references. The obtained results showed good accuracy and detection sensitivity and are comparable with another methods.

  16. Detection of thrombosis and plaque rupture in atherosclerotic rabbit model by using 3.0 T MR imaging

    International Nuclear Information System (INIS)

    Objective: To explore the imaging of the thrombosis after pharmacological triggering of plaque rupture in atherosclerotic rabbit model by using 3.0 T high-resolution magnetic resonance imaging. Methods: Twenty male New Zealand white rabbits were divided into an experimental group (n=16) and a control group (n=4). The aortic wall injuries were induced by an intravascular balloon in experimental group rabbits after high cholesterol diet. The pharmacological triggering with Russell's viper venom and histamine was performed after 3 months of establishment of model. All of the animals underwent pre-trigger and post-trigger MR examinations including 3D time of fight (3D TOF), T1WI, T2WI and post contrast T1WI. Euthanasia was performed in all rabbits and gross anatomy and histological specimen of aorta were obtained. Comparing the location and length of the thrombus between MRI images and histopathology was used Pearson test. Comparing the calculated indexes of abdominal aorta between rabbits with and without thrombosis was used AVONA test and LSD-t test. Results: After triggering, 8 in 14 survived rabbits developed thrombosis in experimental group, meanwhile, no thrombus was found in control group. The accuracy of multi-sequences MRI for detecting of thrombus was 87.1% (27/31). MRI data correlated with the histopathology regarding thrombus length (r=0.85, P2 vs. (8.93±5.36) mm2, P<0.01]. Conclusion: MRI is useful tool to determine the thrombosis and plaque rupture in atherosclerotic rabbit model. (authors)

  17. Combination of poly L-lactic acid nanofiber scaffold with omentum graft for bone healing in experimental defect in tibia of rabbits Combinação de poli L-ácido láctico nanofibras com enxerto de omento para reparo ósseo em defeito experimental em tíbia de coelhos

    Directory of Open Access Journals (Sweden)

    Amir Sotoudeh

    2012-10-01

    Full Text Available PURPOSE: To investigate the osteoconductive properties and biological performance of Poly L-lactic acid (PLLA with omentum in bone defects. METHODS: PLLA nanofiber scaffolds were prepared via electrospinning technique. Forty four New Zealand white female rabbits randomly divided into three groups of 18 rabbits each. Created defects in right tibias were filled in group I with omentum, in group II with PLLA nanofiber scaffold and in group III with combination of the omentum and PLLA. The same defects were created in left tibia of all groups but did not receive any treatment (control group. Histological and histomorphometric evaluations were performed at two, four and six weeks after the implantation. RESULTS: Histological changes on all groups along with the time course were scored and statistical analysis showed that the average scores in group III were significantly higher than the other groups. CONCLUSION: Histomorphometric analysis of bone healing was shown to be significantly improved by the combined PLLA with omentum compared with the other groups, suggesting this biomaterial promote the healing of cortical bone, presumably by acting as an osteoconductive scaffold.OBJETIVO: Investigar as propriedades de osteocondução e desempenho biológico do ácido L láctico-Poly (PLLA com omento em defeitos ósseos. MÉTODOS: Andaimes PLLA nanofibras foram preparados via eletrofiação técnica. Cinquenta e quatro coelhos fêmeas Nova Zelândia brancos foram distribuídos aleatoriamente em três grupos de 18 coelhos cada. Defeitos criados em tíbias direitas foram preenchidos no grupo I com omento, no grupo II com PLLA nanofibras e no grupo III com a combinação do omento e PLLA. Os mesmos defeitos foram criados na tíbia esquerda de todos os grupos, mas não receberam qualquer tratamento (grupo controle. As avaliações histológicas e histomorfométricas foram realizadas em duas, quatro e seis semanas após a implantação. RESULTADOS: As altera

  18. Resveratrol Protects Rabbits Against Cholesterol Diet-Induced Hyperlipidaemia.

    Science.gov (United States)

    Tanko, Y; Jimoh, A; Ahmed, A; Mohammed, A; Ayo, J O

    2016-01-01

    The excessive consumption of high cholesterol diet has been associated with an increased incidence oflipidaemia. Lipidaemia is enhanced by formation of oxidative stress, lipid peroxidation and hyperglycaemia. The aim ofthese experiments was to investigate the protective effect of resveratrol co-administered with cholesterol diet inducedhyperlipidaemia in rabbits. Thirty rabbits divided into six groups of five animal (group= 5) each: group 1 = normal control,group 2 = cholesterol diet/high fat diet group only (HFD), group 3 = resveratrol 200 mg/kg (R200), group 4 = resveratrol400 mg/kg (R400), group 5 = HFD + R200 and group 6 = HFD + R400. The normal group was fed with standard animalfeeds only; while the HFD groups were fed with standard animal feeds + cholesterol diet (10% Groundnut oil, 20%Groundnut mill and 2% cholesterol). Resveratrol-treated rabbits received resveratrol suspended in 10 g/Lcarboxymethylcellulose (CMC) and the control group received the vehicle only, CMC. The preparations were administeredfor 8 weeks of experimental protocol. At the end of the study period, the animals were sacrificed. Blood and plasma sampleswere collected. Serum evaluation of lipid profile such as total cholesterol (TC), triacylglycerol (Tg), low density lipoproteincholesterol (LDP-c) and high density lipoprotein cholesterol (HDL-c) were also assessed. The results obtained showsignificant (P < 0.05) decrease in total cholesterol (TC), Low density lipoprotein cholesterol (LDP-c), total triacylglyceroland an increase in high density lipoprotein cholesterol (HDL-c) in resveratrol treated groups compared to HFD group only.In conclusion, the findings indicated that Resveratrol may contain polar products able to lower plasma lipid concentrationsand might be beneficial in treatment of hyperlipidemia and atherosclerosis. PMID:27574767

  19. Experimental study of Gynostemma pentaphyllum on inhibiting lipid deposition in rabbit liver with nonalcohol fatty liver disease%绞股蓝抑制非酒精性脂肪性肝病兔肝脏脂质沉积的实验研究

    Institute of Scientific and Technical Information of China (English)

    刘其政; 孙希杰; 谭华炳; 吴凡; 胡波

    2011-01-01

    Objective; To study the effect of gynostemma pentaphyllum inhibiting lipid deposition in rabbit liver with nonal-cohol fatty liver disease, and discuss the mechanism. Methods; Forty Japanese adult male white rabbits with NAFLD were randomly divided into intervention group of gynostemma pentaphyllum (Group A) , intervention group of simvastatin (Group B), pattern group (Group C) , the control group (Group E) , respectively every group was given high fat and gynostemma pentaphyllum diet 5g/kg, high fat and simvastatin diet 5g/kg, high fat diet, standard diet, the experimental cycle was 9 weeks. And synchronously tested triglyceride (TG) , cholesterol (TCh) before and after experiment. Made liver tissue homogenate, detected the weight of TG in liver tissue homogenate. Pathological changes of liver tissue were observed in order to realize the degree of NNAFLD . Results; ①TCh and TG level: Before and after the experiment group A, group B and group C there was a significant difference (P < 0. 01) . After the feeding, TG and TCh of group A and group B were lower than group C, there was a significant difference ( P < 0.01) ; the TG of group A were lower than group B, there was a significant difference ( P < 0.01);the TCh of group B were lower than group A, there was a significant difference (P < 0.01) . ②The TG level of liver tissue; The TG of group A and group B were lower than group C, there was a significant difference ( P < 0.01) . The TG of group A were lower than group B, there was a significant difference (P < 0. 01) . ③Liver pathology: The liver of group D was ruddy soft, the livers of group A, B, C were diffuse enlargement, edge blunt and thick, tawny. The colour and lustre of group A was ruddyer than group B and group C; the degree of enlargement was lighter. With the Trypan-blue, structure and cells form of group D was normal, 2/3 above cell of group A changed with adipose kind, one hundred percent nearly cell of group B and group C changed withadipose

  20. Genotoxic effects of copper sulfate in rabbits

    Directory of Open Access Journals (Sweden)

    Georgieva S.

    2013-01-01

    Full Text Available This study was carried out to determine the genotoxic effects of oral application of CuSO4 in rabbits by the chromosome aberration (CA and sister chromatid exchange (SCE tests. Ten male New Zealand rabbits (5 months old, weighing 3.5-4.0 kg were allocated into two groups. The first group received CuSO4 (5H2O in drinking water for 6 consecutive days. The second group was used as a control. On the 7th day, blood samples were taken from the ear marginal vein and the SCE and CA tests in peripheral lymphocytes were used as genotoxicity and mutagenicity endpoints, respectively. Results showed a significant increase in the frequencies of the aberrant cells (7.4±0.24, P<0.001 and CA (chromatid fragments 3.2±0.37, chromosome fragments 4.2±0.37, P<0.001, and total aberrations (7.4±0.24, P<0.001 after the treatment with CuSO4 when compared with the control group. The level of SCE per cell in the CuSO4-treated rabbits (9.66±0.062 was significantly higher than in rabbits from the control group. These findings show that copper exhibits a genotoxic and mutagenic potential in rabbits.

  1. Accumulation of 125I-factor XI in atheroma of rabbit with hereditary hyperlipidemia (WHHL-rabbit)

    International Nuclear Information System (INIS)

    We have studied the turnover and accumulation of rabbit factor XI (F.XI) in atherosclerotic lesion in Watanabe-hereditable hyperlipidemic rabbit (WHHL rabbit) to reveal the participation of blood coagulation in atherosclerotic lesion. Rabbit F.XI was iodinated and administered intravenously to WHHL rabbits and Japanese white rabbits. The turnover of 125I-rabbit F.XI was significantly faster in WHHL rabbits (T1/2 = 2.84 +/- 0.44 days) than in normal rabbits (T1/2 = 4.44 +/- 0.42 days). The thoracic aorta of WHHL rabbit was strongly labelled with 125I-rabbit F.XI, in sections obtained after 5 days by en-face autoradiography, whereas no radioactivity was detected in normal aorta. By an immunohistochemical study of WHHL rabbit aorta, we confirmed that many F.XI- and fibrin-related compounds existed in the atheroma, whereas albumin did not in these area. These results suggest that the activation of F.XI proceeds on the atherosclerotic lesions of WHHL rabbits

  2. Effect of Humulus scandens for Traditional Fiber Sources on Digestion,Diarrhea,and Performance of Growing Rabbits

    Institute of Scientific and Technical Information of China (English)

    GUO Wan-hua; GU Zi-lin; WEI Kun-peng; LIU Ya-juan; WANG Zeng-li

    2009-01-01

    This work was to determine which concentration of a mixture of fresh Humulus scandens(HS)and fresh alfalfa could be included in diets for rabbits.The fresh forage of three experimental diets is composed of 100% alfalfa,50% alfalfa+50% HS,100% HS,respectively.30 rabbits[(1.42±0.056)kg]were used to determine nutrient digestibility.A feeding trial was carried out using 90 weaning rabbits[35 d of age;(512±6.5)g].Increasing content of HS in the diet did not affect digestibility of DM(dry matter),CF(crude fiber),CP(crude protein),GE(gross energy),and EE(ether extract).But diarrhea decreased markedly(P<0.01)compared with the diets that had no HS.ADFI(average daily feed intake)increased by 4.3%(P<0.05)and 3.6%(P<0.05)when rabbits fed the diet with 100% HS of fresh forage compared with the diets containing 0 and 50% HS,respectively.Feed efficiency improved significantly(P<0.05)by 2.07 and 5.30% when rabbits fed 0 and 50% HS of fresh forage,respectively,compared with 100% HS.There was no effect on ADG(average daily weight gain).In conclusion,the mixture(50∶50)of HS and alfalfa constitutes an alternative source of fiber for fattening rabbits and prevents diarrhea effectively.

  3. Effect of calcium citrate on bone integration in a rabbit femur defect model

    Institute of Scientific and Technical Information of China (English)

    Wei Zhang; Peng-Fei Nie; Xiu-Cui Li; Ferdinand An Rompis; Hang Huang; Hua Zhang; Zhong-Lin Mu; Lei Peng; Wei Wang; Qing-Yu Chen; Zhong-Qin Lin; Shao-Wen Cheng; Dong-Quan Kou; Xiao-Zhou Ying; Yue Shen; Xiao-Jie Cheng

    2012-01-01

    ABSTRACT Objective:To explore effect of calcium citrate on bone integration in a rabbit femur defect model, and to compare the bone formation with different sizes by radiological and histological study. Methods:Twenty-four male Japanese white rabbits were randomly divided into three groups (GroupA, B, C) in this study. Under anesthesia, defects of four sizes (1.2, 1.5, 2.0 and2.5 mm) were created in each of the rabbits. Commercially pure calcium citrate powder was placed inside the medullary compartment of the femur (Experimental), while in the contralateral femur (Control) nothing was implanted. The defects were analyzed using radiography and histological analysis by using Imagepro-Plus6.0 software after animal was sacrificed at 4th(GroupA), 6th(GroupB) and8th(GroupC) weeks postoperatively. Four samples were analyzed for each size of defect and each healing period.Results:The histological and the radiologic evaluation were performed after sacrification of all rabbits on postoperative4th and6th weeks, It showed significant difference between the experimental group and the control group when these defects were less than or equal to2.0 mm. No statistical difference was observed when these defects were larger than2.0 mm at all healing periods except at the4th week.Conclusions: Calcium citrate affects the early periods of bone defects healing mechanism in Japanese white rabbits positively, especially when the defect is not too large. We suggest further studies on calcium citrate to determine the effects of various dosages, administration ways and the experimental time on the bone defects.

  4. Diagnosis of dental problems in pet rabbits (Oryctolagus cuniculus)

    OpenAIRE

    Van Caelenberg, Annemie; De Rycke, Lieve; Hermans, Katleen; Verhaert, Maria-Magdalena; van Bree, Henri; Gielen, Ingrid

    2008-01-01

    Dental problems are very common in pet rabbits. To establish a correct diagnosis of rabbit dental pathology, a general knowledge of normal dental anatomy and physiology is necessary. The specific anatomy and the most common pathologies of rabbit dentition are reviewed. Techniques for diagnosing dental abnormalities - such as clinical examination, radiography and computed tomography (CT) - are summarized. Finally two clinical cases of rabbits with dental pathologies are described.

  5. Efficient immunoglobulin gene disruption and targeted replacement in rabbit using zinc finger nucleases.

    Directory of Open Access Journals (Sweden)

    Tatiana Flisikowska

    Full Text Available Rabbits are widely used in biomedical research, yet techniques for their precise genetic modification are lacking. We demonstrate that zinc finger nucleases (ZFNs introduced into fertilized oocytes can inactivate a chosen gene by mutagenesis and also mediate precise homologous recombination with a DNA gene-targeting vector to achieve the first gene knockout and targeted sequence replacement in rabbits. Two ZFN pairs were designed that target the rabbit immunoglobulin M (IgM locus within exons 1 and 2. ZFN mRNAs were microinjected into pronuclear stage fertilized oocytes. Founder animals carrying distinct mutated IgM alleles were identified and bred to produce offspring. Functional knockout of the immunoglobulin heavy chain locus was confirmed by serum IgM and IgG deficiency and lack of IgM(+ and IgG(+ B lymphocytes. We then tested whether ZFN expression would enable efficient targeted sequence replacement in rabbit oocytes. ZFN mRNA was co-injected with a linear DNA vector designed to replace exon 1 of the IgM locus with ∼1.9 kb of novel sequence. Double strand break induced targeted replacement occurred in up to 17% of embryos and in 18% of fetuses analyzed. Two major goals have been achieved. First, inactivation of the endogenous IgM locus, which is an essential step for the production of therapeutic human polyclonal antibodies in the rabbit. Second, establishing efficient targeted gene manipulation and homologous recombination in a refractory animal species. ZFN mediated genetic engineering in the rabbit and other mammals opens new avenues of experimentation in immunology and many other research fields.

  6. Effect of 0.25 T static magnetic field on microcirculation in rabbits.

    Science.gov (United States)

    Gmitrov, Juraj; Ohkubo, Chiyoji; Okano, Hideyuki

    2002-04-01

    We showed previously in rabbits that 0.2 and 0.35 T static magnetic field (SMF) modulated systemic hemodynamics by arterial baroreceptors. We now have measured the effect of 0.25 T SMF on microcirculation within cutaneous tissue of the rabbit ear lobe by the rabbit ear chamber (REC) method. Forty experimental runs (20 controls and 20 SMF) were carried out in eight different rabbits with an equal number of control and SMF experiments on each individual. Rabbits were sedated by pentobarbital sodium (5 mg/kg/h, i.v.) during the entire 80 min experiment. SMF was generated by four neodium-iron-boron alloy (Nd2-Fe14-B) magnets (15 x 25 x 30 mm, Neomax, PIP - Tokyo Co., Ltd., Tokyo, Japan), positioned around the REC on the observing stage of an optical microscope. The direct intravital microscopic observation of the rabbit's ear microvascular net, along with simultaneous blood flow measurement by microphotoelectric plethysmography (MPPG), were performed PRE (20 min, baseline), DURING (40 min), and POST (20 min) magnetic field exposure. The control experiments were performed under the same conditions and according to the same time course, but without magnetic field. Data were analyzed comparing MPPG values and percent change from baseline in the same series, and between corresponding sections of control and SMF runs. In contrast to control series (100+/-0.0%-90.0+/-5.4%-87.7+/-7.1%, PRE-EXPOSURE-POST), after magnetic field exposure we observed increased blood flow (100+/-0.0%-117.8+/-9.6%*-113.8+/-14.0%, *P<0.05) which gradually decreased after exposure cessation. We propose that long exposure of a high level nonuniform SMF probably modifies microcirculatory homeostasis through modulation of the local release of endothelial neurohumoral and paracrine factors that act directly on the smooth muscle of the vascular wall, presumably by affecting ion channels or second messenger systems. PMID:11891752

  7. Culture and characterization of juvenile rabbit tenocytes.

    Science.gov (United States)

    Bernard-Beaubois, K; Hecquet, C; Houcine, O; Hayem, G; Adolphe, M

    1997-02-01

    The culture of rabbit tenocytes could be a useful model in the study of the physiopathology and pharmacotoxicology of tendons. This work was undertaken to examine the in vitro behavior of tenocytes form juvenile rabbit Achilles tendons. We report observations of the morphological and biological characteristics of primary culture and subsequent passages of rabbit tendon cells cultured in monolayer. Data obtained by electron microscopy and growth curves were complementary. After 36 passages, the generation time of tenocytes did not change and no sign of senescence could be seen. Primary culture and the first passages retained the expression of tenocyte differentiated functions, synthesis of type I collagen and decorin. Cell growth behavior was not modified upon passaging. However, when subcultured, tenocytes displayed a modulated phenotype. PMID:9049100

  8. White Rabbit in space related application

    Science.gov (United States)

    JamroŻy, M.; Gumiński, M.; Kasprowicz, G.; Romaniuk, R.; Poźniak, K.

    2015-09-01

    This paper describes study results regarding potential use of White Rabbit technology in Space Related Applications. During the study Technology Readiness Level and Compliance with Space Related Applications was evaluated. After considering possible deployment and development scenarios, main focus has been put on European Space Agency's tracking station system. This outcome derived from specific requirements of tracking system which are coherent with White Rabbit technology scope of application and further development plans. Current state of Time and Frequency Distribution technology implemented into tracking stations is based on multiple different technologies coexisting in parallel creating a complex system. It requires specific, custom made hardware to combine all the technologies which makes it expensive and difficult to maintain. White Rabbit could be use to reduce Time and Frequency Distribution to a single Ethernet based network with link redundancy, payload data transfer and sub-nanosecond accuracy.

  9. EFFECT OF DONOR STRAIN AND MATURATION STAGE OF RABBIT OOCYTES ON RESULTS OF PENETRATION TEST OF RABBIT SEMEN

    OpenAIRE

    Mocé, E.; Vicente, J.S.; R. Lavara

    2002-01-01

    Abstract not available. Mocé, E.; Vicente, J.; Lavara, R. (2002). EFFECT OF DONOR STRAIN AND MATURATION STAGE OF RABBIT OOCYTES ON RESULTS OF PENETRATION TEST OF RABBIT SEMEN. http://hdl.handle.net/10251/10003.

  10. Synergistic activity of rabbit granulocyte peptides against Candida albicans.

    OpenAIRE

    Lehrer, R I; Szklarek, D; Ganz, T; Selsted, M E

    1986-01-01

    Rabbit granulocytes contain six antimicrobial peptides that are structurally homologous to the human neutrophil "defensins." NP-5, a rabbit defensin, lacks significant activity against Candida albicans. Nevertheless, its addition to submicromolar concentrations of rabbit NP-1, NP-2, or NP-3a potentiates their candidacidal effect. Thus, granulocyte defensins can act synergistically against potential pathogens.

  11. Prevalence of Rabbit Hemorrhagic Disease (RHD) in wild rabbits (Oryctolagus cuniculus) in Flanders, Belgium, 1999-2002

    OpenAIRE

    Vandekerchove, D; Roels, S; Butaye, Patrick; Van den Berge, K.; Peeters, J.

    2003-01-01

    During the period of July 1999 through June 2002, carcasses of wild rabbits that had been shot or found dead and livers originating from wild rabbits that had been shot for consumption were collected in Flanders. One hundred and twelve carcasses were suitable for necropsy and histological and bacteriological analysis; histological analysis was possible in 41 livers. Considering the 112 rabbit carcasses only, Rabbit Hemorrhagic Disease (RHD) was found to be present in 33.9% of the cases. RHD w...

  12. Iron supply for erythropoiesis in the rabbit.

    OpenAIRE

    Celada, A; Stray, S; Sivarajan, M; Finch, C

    1984-01-01

    Marrow radioiron uptake and marrow blood flow were measured in order to evaluate iron supply for erythropoiesis. Normal, phenylhydrazine-treated and bled animals were studied. The plasma iron turnover of seven normal rabbits was 1.49 +/- 0.22 mg/dl whole blood per d, of 11 rabbits treated 4 d before with phenylhydrazine was 5.16 +/- 1.81, and of four bled animals the plasma iron turnover was 3.75 +/- 1.61. The cardiac output and the percentage of blood flow to the marrow was increased in phen...

  13. A novel functional rabbit IL- 7 isoform

    OpenAIRE

    Siewe, Basile T.; Kalis, Susan L.; Esteves, Pedro J; Zhou, Tong; Knight, Katherine L.

    2010-01-01

    IL-7 is required for B cell development in mouse and is a key regulator of T cell development and peripheral T cell homeostasis in mouse and human. Recently, we found that IL-7 is expressed in rabbit bone marrow and in vitro, is required for differentiation of lymphoid progenitors to B and T lineage cells. Herein, we report the identification of a novel rabbit IL-7 isoform, IL-7II. Recombinant IL-7II (rIL-7II) binds lymphocytes via the IL-7R and induces phosphorylation of STAT5. Further, rIL-...

  14. Processing of rabbit hemorrhagic disease virus polyprotein.

    OpenAIRE

    Martín Alonso, J M; Casais, R.; Boga, J A; Parra, F

    1996-01-01

    Expression of rabbit hemorrhagic disease virus (RHDV) cDNAs in vitro with rabbit reticulocyte lysates and in Escherichia coli have been used to study the proteolytic processing of RHDV polyprotein encoded by ORF1. An epitope tag was used for monitoring the gene products by a specific antibody. We have identified four gene products with molecular masses of 80, 43, 73, and 60 kDa, from the amino to the carboxy terminus of the polyprotein. The amino-terminal sequences of the 43- and 73-kDa produ...

  15. Isolation and distribution of rabbit keratocyte precursors

    OpenAIRE

    Mimura, Tatsuya; Amano, Shiro; Yokoo, Seiichi; Uchida, Saiko; Usui, Tomohiko; Yamagami, Satoru

    2008-01-01

    Purpose To isolate multipotent precursors from the rabbit corneal stroma and to compare the distribution and proliferative capacity of keratocyte precursors obtained from the central and peripheral regions of the corneal stroma. Methods The rabbit corneal stroma was divided into a peripheral region (6.0–10.0 mm in diameter) and a central region (6.0 mm in diameter). A sphere-forming assay was then performed to isolate precursors from the stroma of each region. To promote differentiation, isol...

  16. DETECTION OF MSTN POLYMORPHISM IN RABBIT

    OpenAIRE

    ALICA RAFAYOVÁ; ZUZANA LIESKOVSKÁ; ANNA TRAKOVICKÁ; A. KOVÁČIK

    2013-01-01

    Myostatin is negative regulation factor, which determine maximal amount of body mass typical for every species. If there is a mutation in myostatine, its negative regulation function is disrupt. MSTN is important candidate gene for meat production. In rabbit it is known sequention of exon 1, 2, 3 and intron 1, 2. No mutation is observed, only one SNP in intron 2, C-T transition in position 34. We analysed 127 broiler rabbits linea M91 and P91 using PCR-RFLP. We noted slight majority of genoty...

  17. Cardiac muscle’s mitochondrial dysfunction in hypercholesterolemic rabbits

    Directory of Open Access Journals (Sweden)

    Kojić Zvezdana

    2006-01-01

    Full Text Available Cardiovascular diseases are often associated with energy deficit and in many cases this is also accompanied by lipid disorders such as hyperlipidemias and obesity. The aim of the study was to check mitochondrial oxidative capacity in the course of twelve weeks atherogenic hypercholesterolic diet. Thirty five Chinchilla rabbits, male, were randomized to one of two groups a control group (A, n=17 received (per os physiological saline experimental group (B, n=18 received atherogenic 2% hypercholesterolemic diet. Isolation of the mitochondrial fraction of the heart was done by the method of Tyler. The oxygen consumption rate was studied in different respiration phases: as basal, unstimulated (V4 and as ADP-stimulated (V3 and expressed as indices: respiratory control ratio (RCR and ADP/O. Hypercholesterolemic atherogenic diet induced profound perturbations in mitochondrial energy metabolism and oxidative capacity. Basal oxygen consumption rate without ADP (V4 and the maximal ADP-stimulated respiration rate (V3 showed a marked reduction (quantitative changes; sensibility of mitochondria to ADP (ADP/O was also reduced (qualitative change in rabbits treated by atherogenic diet (group B compared to controls (group A. Respiratory control ratio was not significantly different among the groups. These results indicate that hypercholesterolemic atherogenic diet impairs mitochondrial oxidative capacity without affecting coupling of oxidative and phosphorilative processes.

  18. Effect of sugarcane biopolymer gel injected in rabbit vocal fold

    Directory of Open Access Journals (Sweden)

    Rodrigo Augusto de Souza Leão

    2014-06-01

    Full Text Available INTRODUCTION: Alterations in the vocal folds that involve volume reduction and glottal closure failure result in exaggerated air escape during speech. For such situations, the use of implants or grafts of different materials has been proposed. OBJECTIVE: To define the effect of sugarcane biopolymer gel when implanted in the vocal folds of rabbits. METHODS: This was an experimental study. The vocal folds of rabbits injected with sugarcane biopolymer and saline solution were histologically evaluated after 21 and 90 days. RESULTS: Mild to moderate inflammation and increased volume were observed in all vocal folds injected with biopolymer, when compared to controls. There were no cases of necrosis or calcification. DISCUSSION: This study showed higher inflammatory reaction in cases than in controls and biopolymer biointegration to the vocal fold. This fibrogenic response with absence of epithelial repercussions suggests that the biopolymer in its gel form can be bioactive and preserve the normal vibratory function of the epithelium. CONCLUSION: We show that in spite of producing an inflammatory reaction in vocal fold tissues, the material remained in vocal fold throughout the study period.

  19. Cloning, Characteristics, and Functional Analysis of Rabbit NADPH Oxidase 5

    Science.gov (United States)

    Chen, Feng; Yin, Caiyong; Dimitropoulou, Christiana; Fulton, David J. R.

    2016-01-01

    Background: Nox5 was the last member of the Nox enzyme family to be identified. Functionally distinct from the other Nox isoforms, our understanding of its physiological significance has been hampered by the absence of Nox5 in mouse and rat genomes. Nox5 is present in the genomes of other species such as the rabbit that have broad utility as models of cardiovascular disease. However, the mRNA sequence, characteristics, and functional analysis of rabbit Nox5 has not been fully defined and were the goals of the current study. Methods: Rabbit Nox5 was amplified from rabbit tissue, cloned, and sequenced. COS-7 cells were employed for expression and functional analysis via Western blotting and measurements of superoxide. We designed and synthesized miRNAs selectively targeting rabbit Nox5. The nucleotide and amino acid sequences of rabbit Nox5 were aligned with those of putative rabbit isoforms (X1, X2, X3, and X4). A phylogenetic tree was generated based on the mRNA sequence for Nox5 from rabbit and other species. Results: Sequence alignment revealed that the identified rabbit Nox5 was highly conserved with the predicted sequence of rabbit Nox5. Cell based experiments reveal that rabbit Nox5 was robustly expressed and produced superoxide at rest and in a calcium and PMA-dependent manner that was susceptible to superoxide dismutase and the flavoprotein inhibitor, DPI. miRNA-1 was shown to be most effective in down-regulating the expression of rabbit Nox5. Phylogenetic analysis revealed a close relationship between rabbit and armadillo Nox5. Rabbit Nox5 was relatively closely related to human Nox5, but lies in a distinct cluster. Conclusion: Our study establishes the suitability of the rabbit as a model organism to further our understanding of the role of Nox5 in cardiovascular and other diseases and provides new information on the genetic relationship of Nox5 genes in different species. PMID:27486403

  20. Consummation in making rabbit model bearing VX2 li8ver tumor and multiple imaging evaluation

    International Nuclear Information System (INIS)

    Objective: To consummate the creation of rabbit model bearing VX2 liver tumor ulteriorly, compare the imaging performance of CT, MRI and DSA in rabbit bearing VX2 liver tumor and evaluate the superiority of each imaging for demonstrating the tumors. Methods: Thirty-six New Zealand white rabbits were enrolled including 12 designated in the fore stage experiment and 24 in the late stage experiment. The grafted VX2 carcinoma was maintained through serial transplantation into the hind limb of the rabbit by subcutaneous injection. In the fore stage experiment, the rabbits were embeded with VX2 tumor while in the late stage experiment, the rabbits were injected with VX2 tumor in the left lobe of the liver. Liver and kidney functions were checked 1 day before the operation and 1 d, 3 d, 1 w and 2 w after the operation in the late stage experiment. CT and Mil imaging were performed in all of the rabbits 2 weeks later, together with the transcatheter arterial super-selection DSA and nanometer magnetic fluid interventional therapy. Results: The successful rates of implantation were 66.7% in the fore stage experiment and 100% in the late stage experiment. Temporarily elevation of the serum ALT, AST levels was observed but reversed within a week with no statistical significance. Bun and Cr also showed no significant changes. The tumors demonstrated as low density on plain CT scan, inconspicuous enhancement on arterial phase image but well discernible fi:om the surrounding liver tissue. On MR imaging the tumors appeared as low signal intenrts on T1WI, slightly high on T2WI (EPI) and markedly high on DWI. DSA revealed the isolated tumor with abundant tumor vessels supplied by enlarged feeding arteries in the left lobe of the liver and high density in the area of tumor after super-selective injection of the nanometer magnetic fluid. Conclusion: The rabbit model with VX2 liver tumor is suitable for interventional experimental study. The injection method is superior to the embedded

  1. Changes in the brain cortex of rabbits on a cholesterol-rich diet following supplementation with a herbal extract of Tribulus terrestris

    OpenAIRE

    Berkman, Zafer; Tanriover, Gamze; Acar, Goksemin; Sati, Leyla; Altug, Tuncay; Demir, Ramazan

    2009-01-01

    Extracts of the medicinal herb Tribulus terrestris (TT) are used for treating various diseases. The saponins, a component of TT, play a role in regulating blood pressure and in treatment of hyperlipidemia. The aim of the study was to investigate the immunohistochemical and ultrastructural alterations in the cerebral cortex of experimental rabbits on a cholesterol rich diet treated with TT. The rabbits were divided into three groups and followed for 12 weeks as control ...

  2. Possible interaction between myxomatosis and calicivirosis related to rabbit haemorrhagic disease affecting the European rabbit.

    Science.gov (United States)

    Marchandeau, S; Bertagnoli, S; Peralta, B; Boucraut-Baralon, C; Letty, J; Reitz, F

    2004-11-01

    Serological data on myxoma virus, rabbit haemorrhagic disease (RHD) virus and RHD-like viruses in juvenile rabbits (Oryctolagus cuniculus) trapped in 1995, 1996 and 1997 in two areas of France were analysed. For each disease, the effects of bodyweight, year, month and seropositivity for the other disease were modelled by using logistic regressions. In one area, a model including RHD seropositivity was selected to explain the myxoma virus seropositivity. Models including myxoma virus seropositivity were selected to explain the RHD seropositivity in both areas, and the odds of a rabbit being seropositive to both viruses were 5.1 and 8.4 times higher than the odds of a rabbit being seronegative to myxoma virus and seropositive to RHD. The year and bodyweight had significant effects for myxomatosis in one area and for RHD in both areas. PMID:15573951

  3. Hematological Parameters and Carcass Characteristics of Weanling Rabbits Fed Sesame Seed Meal (Sesamum indicum in a Semi-Arid Region

    Directory of Open Access Journals (Sweden)

    A. A. Njidda* and C. E. Isidahomen1

    2011-01-01

    Full Text Available The effect of replacing groundnut cake with Sesame seed meal (Sesamum indicum on hematology, blood chemistry and carcass characteristics was evaluated in rabbits. Sixteen (New Zealand white rabbits were randomly assigned to four dietary treatments in a completely randomized design with four rabbits per treatment. The rabbits were fed diets containing 0, 4, 8 and 12% of sesame seed meal (SSM and designated as T1, T2, T3 and T4, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were selected for hematological analysis and carcass evaluation. There were significant differences (P0.05 by the levels of SSM in the diets. There were significant differences (P0.05 on serum albumin and total proteins among treatments. The carcass characteristics also showed significant differences (P0.05 on the hematological parameters, serum biochemical indices and carcass characteristics.

  4. 兔慢性上颌窦炎窦口鼻道复合体不同处理方式对窦黏膜纤毛形态与功能转归影响的观察%Effects of different treating ways to the ostiomeatal complex on changes in ciliary morphology and function of sinuous mucosa in rabbits with experimental chronic sinusitis

    Institute of Scientific and Technical Information of China (English)

    林彬; 王挥戈

    2011-01-01

    目的 观察功能性鼻内镜术后鼻窦黏膜纤毛形态与功能的转归状况,为术中及术后处理策略提供实验依据.方法 新西兰大白兔36只,建立慢性上颌窦炎模型.然后,按术中及术后处理方式的不同分成4组,即窦口开放组12只,窦口扩大组12只,手术对照组6只,空白对照组6只.术后12周处死动物,纤毛传输速率检测,并在相差显微镜下观测纤毛活性,获取标本进行细菌培养及窦腔黏膜纤毛形态学观察.结果 上颌窦分泌物细菌培养结果,窦口开放组阳性率100%(12/12),显著高于窦口扩大组的42%(5/12);窦口开放组纤毛传输速率平均2.2mm/min(0~6.2mm/min),窦口扩大组为5.2mm/min(2.1~10.1mm/min).结论 功能性鼻内镜术后,上颌窦腔黏膜纤毛活性和清除功能转归与黏膜组织结构基本同步.手术的关键应是解决窦口的持续通畅引流,有限度的窦口扩大术式明显优于单纯的窦口开放.%Objective To investigate the effects of different treating ways during the period of functional endoscopic sinuous surgery (FESS) to ostiomeatal complex (OMC) on the recovery of ciliary morphology and function in sinuous mucosa in rabbits with experimental maxillary sinusitis in order to offer experimental evidences for the treatment of this type of sinuous lesion during and after the procedure of FESS. Method Thirty-six New Zealand white rabbits were taken to prepare the model of chronic maxillary sinusitis at first. Then, they were divided into 4 groups on the basis of treating way to COM, i.e. 12 animals with their ostium opened (OO), 12 with their ostium enlarged (OE),6 as sham-operation control group (SCG), and 6 as blank control group (BCG). By the 12th week following the surgery, all these animals were put into death to assay their mucociliary transporting rate (MTR) and ciliary beat rate observed under contrast phase microscope and to obtain samples for bacteria culture and cilia morphological observation

  5. Towards a unique and transmissible vaccine against myxomatosis and rabbit haemorrhagic disease for rabbit populations

    OpenAIRE

    Angulo, Elena; Bárcena, Juan

    2007-01-01

    Currently available vaccines against myxomatosis and rabbit hemorrhagic disease virus (RHDV) are not suited to immunise wild rabbit populations, as vaccines need to be delivered individually by conventional veterinary practices. As an alternative approach, research in Spain has focused on the development of a transmissible vaccine. A recombinant virus has been constructed based on a naturally attenuated myxoma virus (MV) field strain, expressing the RHDV capsid protein (VP60). Following inocu...

  6. Técnica de separação da membrana de Descemet para transplante de células endoteliais da córnea: estudo experimental em coelhos Technique for separating Descemet membrane for corneal endothelial cells transplantation: experimental study in rabbits

    Directory of Open Access Journals (Sweden)

    Daniel Wasilewski

    2010-02-01

    Full Text Available OBJETIVO: Avaliar a porcentagem de dano endotelial induzido por uma técnica cirúrgica para a separação da membrana de Descemet contendo endotélio sadio, analisar a viabilidade e eficácia desta técnica, e avaliar a porcentagem de dano endotelial causado pela inversão da córnea em câmara anterior artificial. MÉTODOS: As córneas de três grupos de 12 coelhos da linhagem Nova Zelândia foram avaliadas. O grupo 1 foi usado como controle; portanto, as córneas foram analisadas após coletadas e trepanadas. O grupo 2 foi analisado após a inversão da córnea (endotélio para cima na posição convexa, montada em câmara anterior artificial, para o cálculo da porcentagem do dano endotelial induzido por esta inversão. O grupo 3 foi avaliado após a separação entre a membrana de Descemet e o estroma com o uso de substância viscoelástica em córneas invertidas e montadas em câmara anterior artificial. O dano endotelial foi avaliado por meio de fotografias digitais tiradas no microscópio após impregnar o endotélio com vermelho de alizarina. Amostras do grupo 3 foram processadas para avaliação histopatológica. RESULTADOS: O grupo 3 (separação viscoelástica apresentou um índice de lesão celular endotelial de 10,06%, o grupo 2 apresentou um índice de 3,58% e o grupo controle um índice de 0,18% de lesão celular endotelial (pPURPOSE: To evaluate the percentage of endothelial cell damage induced during a surgical technique of Descemet's membrane separation containing healthy endothelium, analyze the viability and efficacy of this technique, and evaluate the percentage of endothelial cell damage caused by inversion of the cornea on an artificial anterior chamber. METHODS: The corneas from three groups of 12 New Zealand rabbits were evaluated. The Group one was used as the control, so the corneas were analyzed after collected and trephinated. The Group two was analyzed after inversion of the cornea (endothelial side up at a convex

  7. Becoming and Being: "The Velveteen Rabbit."

    Science.gov (United States)

    Jordan, Anne Devereaux

    1997-01-01

    Defends "Velveteen Rabbit," a beloved classic, on its 75th anniversary. Finds that the story of a toy which becomes real has stood the test of time, even though critics call it overly sentimental. States that it deals with a universal theme and prepares its young readers for life's vicissitudes. Lists the book's classic elements. Gives questions…

  8. Lewis Thomas and droopy rabbit ears

    OpenAIRE

    Bashyam, Hema

    2007-01-01

    In 1956, Lewis Thomas discovered that injecting rabbits with a plant protease called papain caused their ears to collapse. This experiment led to the identification of the endogenous proteases that cause the tissue destruction seen in diseases such as rheumatoid arthritis.

  9. Dystocia in a rabbit (Oryctolagus cuniculus)

    OpenAIRE

    Dickie, Erica

    2011-01-01

    A 4-year-old female dwarf lop rabbit was presented with dystocia after mis-mating. Abdominal palpation, vaginal examination, and radiography confirmed that the doe was carrying 3 kits. Treatment for the dystocia consisted of gentle manual extraction of the fetuses and fetal membranes, and administration of oxytocin and calcium borogluconate.

  10. Rabbit production and rabbit market in Romania : the most frequent breeds, half-breeds and their characterization

    OpenAIRE

    Blaga, Bianca-Claudia; Burny, Philippe

    2014-01-01

    This study has in view to investigate grossly the Romanian rabbit market as starting point for a future deeper and larger economic approach of the Romanian rabbit market as part of the European market. In Romania, the rabbit meat is expensive (4.48-9.01 Euro perkg), and this is due to its lownumber of consumers. The local rabbit production in Romania is supported only by small farms, and these are not competitive. Rabbit meat imported from Spain is con siderably cheaper than the one local pro...

  11. Effect of Chinese herbal compound on liver fibrosis in rabbits with schistosomiasis by B-ultrasound

    Institute of Scientific and Technical Information of China (English)

    Xiao-Lu Liang; Jia-Ying Yuan

    2013-01-01

    Objective:To explore the value of B-ultrasound on the evaluation of the effects of traditional Chinese medicine compound of Radix astragali, Salvia miltiorrhiza and Angelica sinensis, and TCM +praziquantel on liver fibrosis in rabbits with schistosomiasis. Methods: The hepatic fibrosis model in rabbits with schistosomiasis was established. The experimental animals (24 rabbits) were randomly divided into four groups (group A, B, C and D, n=6). Group A (control group) was only treated by praziquantel; Group B was treated by mixture of Radix astragali and Salvia miltiorrhiza +praziquantel; Group C was treated by mixture of Radix astragali and Angelica sinensis +praziquantel; Group D was treated by mixture of Radix astragali, Salvia miltiorrhiza and Angelica sinensis +praziquantel. Then B-ultrasonogram was used to evaluate the effects. Results: Each group showed certain curative effect on liver fibrosis in rabbits with schistosomiasis. The efficacy of group B, C and D was better than group A, and that of group D was the best. The differences in long diameter, thickness diameter, transverse diameter and portal vein inner diameter of liver before and after treatment were statistically significant (P<0.05). The liver function indexes and liver fibrosis indexes were significantly improved after treatment (P<0.05). Conclusions:The mixture of Radix astragali, Salvia miltiorrhiza and Angelica sinensis combined with Western medicine treatment can obviously improve the efficacy on liver fibrosis of schistosomiasis.

  12. Influence of habitat modification on the intestinal helminth community ecology of cottontail rabbit populations.

    Science.gov (United States)

    Boggs, J F; McMurry, S T; Leslie, D M; Engle, D M; Lochmiller, R L

    1990-04-01

    The influence of five brush management treatments using the herbicides tebuthiuron and triclopyr, with or without prescribed burning, on the intestinal helminth community of cottontail rabbits (Sylvilagus floridanus) was studied in 1987 on the Cross Timbers Experimental Range in Payne County, Oklahoma (USA). Six helminth species were found (Dermatoxys veligera, Trichostrongylus calcaratus, Passalurus nonanulatus, Wellcomia longejector, Taenia pisiformis cystercercus, and Mosgovoyia pectinata americana) in 102 rabbits (88 adult and 14 juveniles) collected over two seasons (winter and summer). Prevalence of M. pectinata americana in cottontail rabbits was significantly greater in untreated control pastures than herbicide treated pastures in winter, while prevalence of T. pisiformis was significantly greater in burned than unburned pastures. Abundances of helminth species in the intestinal tract of cottontail rabbits were unaffected by brush treatments. Mosgovoyia pectinata americana abundance demonstrated a highly significant increase from winter to summer; conversely, abundance of all oxyurid pinworms combined (D. veligera, P. nonanulatus, W. longejector) was significantly higher in winter than summer. Helminth community dynamics were significantly influenced by season, but were unaffected by brush treatments. Habitat modification could have influenced cestode transmission by altering the ecology of invertebrate and vertebrate hosts. PMID:2338720

  13. Immune response to dehydrated human dura mater : evaluation in a rabbit model.

    Directory of Open Access Journals (Sweden)

    Kadioglu H

    2002-07-01

    Full Text Available Ninety white hybrid rabbits, each weighing 2.5 to 3.5 kg, were used for this experimental model. Thirty rabbits were used for control, and sixty other rabbits were investigated for the response of host to the dural graft. In all animals, a dural defect, 1 x 1 cm in size, was created on the left parietal area following craniotomy. In the control group the excised free dural piece was then sutured again to the area from which it had been excised before. The dural defect was closed with dehydrated human dura mater (DHD in the half of the rabbits in the group of study, and with autogenous fascia lata (AFL in the other half. After operation, animals in each group were then subjected to one of five different groups comprising of 3,14,30,60 and 90 days follow-up periods. At the end of follow-up periods, histological, parameters such as cellular inflammatory response, development of fibrous tissue, capsulation, and calcification were examined in specimens obtained from the animals. There was no significant difference between AFL and DHD grafts. In conclusion, it seems that DHD is suitable as an ideal dural graft, because the immune response of host to DHD was almost similar to AFL.

  14. EFFECT OF CONSUMPTION OF HEAVY METALS CONTAMINATED FISH (TILAPIA OREOCHROMIS ON METABOLIC PARAMETERS IN RABBITS.

    Directory of Open Access Journals (Sweden)

    Bolawa O.E

    2013-08-01

    Full Text Available This research paper presents the biochemical effect of the consumption of heavy metals contaminated fish on metabolic parameters in rabbit. Total glucose, cholesterol, protein and levels of alkaline phosphate, alkaline aminotransferase (ALT together with aspartate aminotransferase (AST in the serum were measured. Compared with the control a significant decrease of total protein and total cholesterol (p was ascertained in the serum of the experimental groups. Total glucose level was level was significantly increased in the serum of the experimental (p . The values of alkaline phosphate, ALT and AST significantly increased in the serum of the groups. The above results on the biochemical consumption profile indicate the toxic effect of the consumption of these contaminated fishes in rabbits.

  15. A simple method of infecting rabbits with Bovine herpesvirus 1 and 5.

    Science.gov (United States)

    Valera, A R; Pidone, C L; Massone, A R; Quiroga, M A; Riganti, J G; Corva, S G; Galosi, C M

    2008-06-01

    This report describes an alternative technique to inoculate rabbits and to reproduce infection by Bovine herpesvirus 1 and 5. First, the nostrils are anaesthetized by aspersion with local anaesthetic. A few seconds later, and after proving the insensitivity of the zone, the rabbits are put on their back legs with their nostrils upwards and the inoculum is introduced slowly into each nostril by using disposable droppers. Clinical signs, viral isolation from nasal swabs, histological lesions found, positive polymerase chain reaction and antibodies production confirm the infection. This very simple and bloodless technique, where the animals are exposed to minor distress, may be useful for evaluating the virulence of BoHV-1 and BoHV-5 strains, to study the establishment of latent virus infection and to test the potential of experimental vaccines or properties of antiviral drugs. It may be also suitable for experimental infection with other respiratory viruses in this animal model. PMID:18423632

  16. Effect of dietary inclusion of sorghum milling waste on growth response, nutrient utilisation, gut characteristics and cecal microflora of weaner rabbits.

    Science.gov (United States)

    Oso, Oladele A; Sobayo, Richard; Jegede, Vincent; Fafiolu, Adeboye; Iyasere, Oluwaseun Serah; Dele, Peter; Bamgbose, Adeyemi; Cecilia, Adesida

    2011-06-01

    Growth response, nutrient digestibility and cecal microflora of 80 male, mixed breed weaner rabbits fed with varying dietary inclusions of sorghum milling waste (SMW) was investigated. Four experimental diets were formulated such that SMW was included at 0 (control), 100, 200 and 300 g/kg, respectively. Each dietary treatment was performed on 20 rabbits. Feed intake increased (P < 0.05) while final live weight and feed conversion ratio of rabbits decreased (P < 0.05) following increased dietary inclusion of SMW. Rabbits fed with 100 and 200 g/kg SMW had similar feed conversion ratios, weight gain, crude fiber, dry matter and crude protein digestibility values. Rabbits fed with 300 g/kg SMW recorded the lowest (P < 0.05) hot carcass weight, dressing percentage and rack weight. Similar dressing percentage and rack weight were recorded for rabbits fed with control diet, 100 and 200 g/kg SMW. The weight of cecal content increased (P < 0.05) with increased dietary inclusion levels of SMW. Rabbits fed with 300 g/kg SMW recorded the lowest (P < 0.05) coliform and lactobaccillus counts. Dietary inclusion of up to 200 g/kg SMW supported improved growth response and carcass yield without imposing any detrimental effect on cecal microflora. PMID:21615842

  17. Análisis de la situación epidemiológica en una colonia de producción intensiva de conejos para la experimentación entre 1993 e 2007 - Analysis of the epidemiological situation in a colony of intensive production of rabbits for experimentation during 1993 and 2007

    Directory of Open Access Journals (Sweden)

    Pérez Martínez, Ileana

    2010-02-01

    Full Text Available ResumenEl conejo es una especie cotizada por el alto valor nutritivo de su carne, su fácil manejo y alta prolificidad, además puede ser utilizado en la investigación biomédica.SummaryThe rabbit is a species listed by the high nutritional value of their meat, easy handling and high prolificacy, and can be used in biomedical research.

  18. Nuclear imaging of hepatic impact injury on rabbits

    International Nuclear Information System (INIS)

    Objective: To investigate the effect and clinic application value of nuclear imaging on hepatic impact experiment. Methods: Experimental rabbits were impact injured on liver with BIM-IV bio-impact machine. Liver imaging was performed with sodium phytate labeled by 99mTc. Liver blood pool imaging was performed with labeled red blood cells. The results of imaging were similar with the results of anatomy. Results: There were significant difference between normal liver and injured liver. Radio diminution and defect were showed on injured liver areas in labeled hepatic cells. Many types of abnormal radioactivity distribution were observed in liver pool imaging. The results of liver imaging and liver blood pool imaging were corresponded to the results of anatomy. Conclusion: Changes of hepatic cell structures and function after injury could be showed by nuclear imaging. Nuclear imaging was valuable in determining injured liver position or injured degree

  19. Retinal lesions induced on rabbits by single and low energy laser irradiations: histological and ultrastructural study

    International Nuclear Information System (INIS)

    The aim of this histopathological study is triple to specify the retinal layers damaged by each radiant exposure studied, to follow the healing reactions with observations carried out at different time intervals, to determine the minimal radiant exposure to produce a cytological damage visible with the electron microscope. The experimental animals are adult Fauve de Bourgogne rabbits. Two different laser sources were used : a pulsed dye laser and a frequency-doubled YAG laser

  20. 166Ho-chitosan as a radiation synovectomy agent - antigen-induced arthritis in rabbits

    International Nuclear Information System (INIS)

    Radiation synovectomy is a noninvasive therapy that has been investigated as an alternative to surgical synovectomy. It has been successfully employed in the treatment of synovitis in rheumatoid arthritis and other inflammatory arthropathies. In this study, we developed experimental animal model for radiation synovectomy. A model system in which a single injection of ovalbumin into the knee joints of previously sensitized rabbits consistently produced a chronic arthritis which was histologically similiar to human rheumatoid arthritis. (author). 8 refs., 8 figs

  1. ALTERATIONS IN TOTAL PROTEIN CONCENTRATION, SERUM PROTEIN FRACTIONS AND ALBUMIN/GLOBULIN RATIO IN HEALTHY RABBITS

    OpenAIRE

    Nuzhat Sultana; Rahila Najam

    2013-01-01

    This study assessed the effect of oral administration of Aloe vera and was to evaluate total serum protein, albumin and globulin concentrations as well as albumin / globulin (A / G) ratio. Twenty rabbits weighing 1000 – 1800 g were divided into 2 groups. Each group consisted of ten animals. One served as control and other group served as experimental group. Results show that animals after 07, 15 and 30 days dosing of Aloe vera showed highly significant decrease in total protein and globulin a...

  2. Crossbreeding effects on rabbit reproduction from four maternal lines of rabbits.

    Science.gov (United States)

    Ragab, M; Sánchez, J P; Mínguez, C; Baselga, M

    2016-07-01

    Litter size is essential for an efficient production of rabbit meat. A diallel cross between four maternal lines was carried out and the analysis of the components of litter size has been already done. This paper presents the analysis of litter size traits themselves (total born (TB), number born alive (NBA), number weaned (NW)) and kindling interval (KI), that complete the analysis of the reproductive performance. The 16 genetic groups were distributed in four Spanish farms. The V line was present in all farms in order to be used as reference group. A total of 34 546 parities from 7111 does, were analysed. The crossbreeding parameters were estimated according to Dickerson model. The differences between lines performance were of low magnitude and not significant for litter size traits. The LP line showed the shortest KI followed by H respect to lines A and V. These differences reflected the differences between direct and maternal genetic effects. The differences between the average of all crosses and line V were found to be significant and seemed to be important, being 0.46 for TB, 0.56 for NBA, 0.75 for NW and -2.21 days for KI. The differences between reciprocal crosses for litter size were of low magnitude and non-significant, which indicate that the maternal effects are not important between these lines. In general, the lines did not show significant differences in direct and maternal genetic effects for TB, NBA and NW but there were some significant differences for KI, which ranged from 1.54 to 6.85 days in direct effects and from 0.63 to 3.38 days for maternal effects. A positive and, in some cases, relevant heterosis was found. The largest heterosis was for TB in the HV cross (1.05 rabbits), followed by the AH (0.74 rabbits), AV (0.57 rabbits) and LH (0.55 rabbits) crosses. For NBA, significant heterosis was found in HV (1.11 rabbits) and AV (0.49 rabbits) and for NW in AV (0.90 rabbits), LH (0.70 rabbits) and LV (0.58 rabbits). Favourable and significant

  3. Comparison of diagnostic value of duplex and color Doppler ultrasound in experimental acute renal failure in rabbits%二维超声、频谱及彩色多普勒超声在评价实验性兔急性肾衰竭中的价值比较

    Institute of Scientific and Technical Information of China (English)

    黄晓玲; 高云华

    2000-01-01

    Objective To compare the diagnostic value of two-dimentional ultrasound, spectrum and color Doppler ultrasound in experimental acute renal failure(ARF)in rabbits.Methods 50%glycerin(12~15 ml/kg)were injected into the thigh muscle of the back leg of the rabbit to induce an animal model of ARF. Ultrasonic examination was performed on the day before(T0)and days 1,3…13 after the injection(T1,T3… T13).Renal size was evaluated by two-dimensional ultrasound.The hemodynamic changes of the kidney were quantitatively analysed by spectrum Doppler, while color Doppler flow imaging(CDFI)and color Doppler power imaging(CDPI)were used to qualitatively study the blood perfusion of the kidney during ARF. The renal imaging in CDFI and CDPI were classified three grades.Grade I, the blood perfusion of the kidney was normal and the flow singles could be seen at the site near to the renal capsule.Grade II, the blood perfusion of the kidney was slightly abnormal and the flow singles could be seen in the renal cortex but not near to the capsule. Grade Ⅲ, the blood perfusion was heavily abnormal and the flow singles could not be seen in the renal cortex.Results The changes of renal hemodynamics and renal size began at T3,peaked at T5 and T7,and returned to normal at T9. The renal imaging of all rabbits were grade I in CDPI and CDFI at T0.In CDFI, all were gradeⅢat T1~T9, at T11 and T13 most of them only returned to grade Ⅱ, no one returned to grade I.In CDPI, however, all were grade Ⅱ at T1 and T3,while only a few were grade Ⅲ at T5 and T7,and most have returned to grade Ⅰ at T9. Conclusions During the period of ARF, two-dimensional ultrasound is useful in showing the echo and measuring the depth of renal cortex.Spectrum Doppler is helpful in quantitatively analysing the renal hemodynamic hanges. CDPI is more sensitive than CDFI in showing the blood perfusion of the kidney%目的 比较二维超声、频谱及彩色多普勒超声在诊断

  4. 淫羊藿配合髓芯减压术治疗兔激素性股骨头坏死的实验研究%The experimental study of therapeutic effect of epimedium combined with core decompression on hormonal femoral ;head necrosis in rabbits

    Institute of Scientific and Technical Information of China (English)

    刘志国; 马文海; 李杰

    2014-01-01

    Obj ectiev To investigate the therapeutic effect of epimedium combined with core decompression on hormonal femoral head necrosis in rabbits ,and to explore its action mechanism .Methods Sixty New Zealand white rabbits were randomly divided into control group ( n =12 ) and experimental group ( n =48 ) .The animal models with hormonal femoral head necrosis were established by the modified method of horse serum plus methylprednisolone , then according to different therapeutic methods the model animals were divided into model group , operation group ( core decompression ) , traditional Chinese medicine ( TCM ) group ( epimedium ) , integration group ( core decompression +epimedium ) .After 10-week treatment,the bone mineral density,biomechanics of femoral head were examined ,and the expression levels of IGF1, BMP2 mRNA and protein were detected by RT-PCR and Western Blot .Results As compared with those in control group ,the bone mineral density and biomechanical properties of femoral head were significantly decreased in model group , which were obviously improved in TCM group,operation group and integration group ( P 0.05).The expression levels of IGF1,BMP-2 mRNA and protein in TCM group and integration group were significantly higher than those in model group and operation group ( P <0.05).The expression of IGF1 in femoral head was positively correlated to that of BMP-2 mRNA and protein ( P <0.01).Conclusion Epimedium plays an important role in reparation of hormonal femoral head necrosis through upregulating the expressions of IGF 1 and BMP-2.Moreover epimedium combined with core decompression can effectively increase bone mineral density ,enhance bone strength ,which would be an effective therapy for hormonal femoral head necrosis .%目的:观察淫羊藿配合随芯减压术对兔激素性股骨头坏死的治疗效果并探讨其作用机制。方法60只新西兰大白兔随机分为对照组( n =12)和实验组( n =48)。用改进的马血清加

  5. Effects of Clenbuterol,Genotype,and Feeding Method on Bodyweight Gain of Meat Rabbits

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Four-factor and three-level orthogonal experimental design(L9(34))was used in the experiment.The effects of three genotypes:Germany SAB three-bred-cross rabbits(S2),New Zealand purebred(N),and crossbred rabbits(ON);three levels of Clenbuterol treatment: 1 ng.g-1,2 ng.g-1and 3 ng.g-1,and three feeding methods:5-day,7-day and 10-day withdrawal period after two weeks of feeding Clenbuterol(CL)on bodyweight gain were evaluated.CL apparently improved average daily gain(ADG)of rabbits.70-day ADG of 5-day and 10-day withdrawal were higher than that of 7-day withdrawal,70-day ADG of S2 genotype was significantly higher than that of the control and N genotype was significant.Genotype,additive dose and feeding method had significant effects on overall-stage ADG.ON genotype,2 ng.g-1 dose and 5-day withdrawal feeding method was the best.Responses of different genotype to Clenbuterol appeared different at experimental prophase or whole experimental period.

  6. Digestibility of diets containing an antibiotic, a probiotic and a prebiotic in growing rabbits

    Directory of Open Access Journals (Sweden)

    Euclides Braga Malheiros

    2008-12-01

    Full Text Available This study aimed to evaluate the effects of a probiotic and/or prebiotic additive on the nutrient digestibility of diets for growing rabbits. Twenty 50-day-old rabbits were used. A completely randomized experimental design, with five treatments and four replicates each, was used. The applied treatments (T were the following: T1 = negative control diet (no growth promoter; T2 = diet with antibiotic, positive control (0.05% zinc bacitracin; T3 = diet with probiotic (0.15% Bacillus subtillis at a concentration of 109 UFC/g; T4 = diet with prebiotic (0.15% phosphorylated mananoligossaccharide at 30%; and T5 = diet with symbiotic (probiotic + prebiotic. The experimental period lasted 12 days, seven for adaptation to the experimental diets and to the cages, and five days of feces collection. The apparent digestibility coefficients of dry matter, organic matter, crude protein, neutral detergent fiber, acid detergent fiber, mineral matter, and ether extract were calculated. The addition of a probiotic and of a symbiotic to the rabbit diet resulted in digestibility similar to that of animals fed diets containing antibiotics or no additive at all. The best digestibility results were obtained with the prebiotic.

  7. Effect of topical 0.5% povidone-iodine compared to 5% natamycin in fungal keratitis caused by Fusarium solani in a rabbit model: a pilot study Efeito tópico de iodo-povidona 0,5% comparado com natamicina 5% em ceratites fúngicas causadas por Fusarium solani em um modelo animal de coelhos: estudo piloto

    OpenAIRE

    Lauro Augusto de Oliveira; Thales Takeo Takata; Alvio Isao Shiguematsu; Luiz Alberto Soares Melo Júnior; Olga Fischman Gompertz; Luciene Barbosa de Sousa; Mannis, Mark J.

    2008-01-01

    PURPOSE: To evaluate the efficacy of topical administration of 0.5% povidone-iodine in experimental Fusarium solani keratitis in rabbits. METHODS: Fungal keratitis caused by Fusarium solani was induced in the right eye of 24 New Zealand rabbits. The rabbits were randomly divided into 3 different treatment groups: Group I (povidone-iodine) - treated with topical 0.5% povidone-iodine; Group II (natamycin) - treated with topical 5% natamycin; and Group III (control) - treated with topical saline...

  8. Detection of vulnerable plaque by using TWRI in response to pulsed laser irradiation of a MION-laden rabbit artery

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jihoon [Northwestern University, Evanston, IL (United States); Oh, Junghwan [Pukyong National University, Busan (Korea, Republic of)

    2010-01-15

    Thermal wave radiometric imaging (TWRI) of monocrystalline iron oxide nanoparticles (MION)-injected Watanabe heritable hyperlipidemic (WHHL) rabbit arteries was performed, and a phase difference of 0.1 {+-} 0.08 [deg] over background values on TWRI demonstrated the presence of MION. In addition, an analytical solution was derived to describe the experimental TWRI results. In conclusion, TWRI of MION-injected WHHL rabbit arteries in response to pulsed laser irradiation resulted in the temperature increase and thermal wave generation due to the laser energy absorbed by the MION in the artery. TWRI can be utilized for biomedical applications such as vulnerable plaque detection.

  9. Characterization of rabbit CD5 isoforms.

    Science.gov (United States)

    Pospisil, Richard; Kabat, Juraj; Mage, Rose G

    2009-08-01

    Previously described polyclonal or monoclonal antibodies (mAb) to rabbit CD5, raised against expressed recombinant protein or peptides, recognize CD5 on most rabbit B cells. The mAb KEN-5 was originally reported to recognize rabbit CD5. However, KEN-5 binds almost exclusively to T cells and only to a minor population of B cells. We show here that by Enzyme-linked Immunosorbent Assay (ELISA), KEN-5 binds to recombinant rabbit CD5. This interaction is partially inhibited by polyclonal goat anti-CD5 antibody. In addition, immunoprecipitations from lysates of surface biotinylated rabbit lymphocytes with KEN-5 or our anti-CD5 mAb isolate molecules that migrate identically on gels with the same approximate relative molecular mass of 67,000 M(r). By flow cytometric analyses of individual cells from spleen, thymus and appendix, KEN-5 recognizes CD5-like molecules mainly on T cells and on 3-6% of IgM(+) B cells. Immunohistochemical staining of splenic and appendix tissues and confocal immunofluorescent imaging confirm and extend results from flow cytometric analyses. Quantitation of fluorescent colocalization indicates that staining by KEN-5 colocalizes with staining by anti-CD5 on small percentage lymphocytes in splenic tissue sections. As CD5 has both N- and O-linked glycosylation, we hypothesised that differential binding of KEN-5 to T cells and B-cells may be explained by different glycan structures on the CD5 present on T compared to B cells. This hypothesis is supported by ELISA data that show that deglycosylation diminishes the binding of KEN-5 to recombinant rabbit CD5. Screening KEN-5 on an array with 406 glycans was inconclusive. Although we did not identify a strongly binding glycan structure, the data are suggestive that the epitope recognized by KEN-5 may be influenced by glycan structures. The epitope this mAb recognizes may either be the glycan itself, or more likely, is influenced by neighboring glycan structure. Our findings suggest that development

  10. Anti diabetic effect of Momordica charantia (bitter melone on alloxan induced diabetic rabbits.

    Directory of Open Access Journals (Sweden)

    Yakaiah Vangoori, Mishra SS, Ambudas B, Ramesh P, Meghavani G, Deepika K, Prathibha A

    2013-02-01

    Full Text Available Objective: to investigate the anti diabetic effect of the bitter melon on Alloxan induced diabetes in experimental animals (rabbits. Materials and Methods: the alcohol extract of whole fruit was tested for its efficacy in Alloxan (150mg/kg induced diabetic rabbit. The diabetic rabbits were divided into 5groups. Group I (control received 2% gumacasia, groupie (positive control received standard drug Metformin (62.5mg+2%GA, group III, IV, V (T1 T2 T3 were treated orally with a daily dose of 0.5(gm 1gm, 1.5gm respectively for 35 days, for all diabetic rabbits after giving TEST,NC,PC preparations, the blood samples were collected and determined the blood glucose level 0,1,3,24hrs intervals. 0hr reading is before drug giving and remaining 3 readings after drugs giving. 24th her reading is considered as 0hr reading for the next day. Results: administration of alcohol of an extract of bitter melon produced a dose dependent decrease in blood glucose levels in Alloxan induced rabbits. There was a significant fall in blood sugar level in High dose (1.5GM/kg in comparison to low dose (0.5gm/kg and median dose (1gm/kg shown by LSD test. This is comparable to the effect of Metformin. Conclusion: the results of this study show that chronic oral administration of an extract of Momordica charantia fruit at an appropriate dosage may be good alternative anti diabetic agent.

  11. Evaluation of millet residue meal based diets as feed for the domestic rabbit (Oryctolagus cuniculus

    Directory of Open Access Journals (Sweden)

    P. K. Karikari,

    2011-03-01

    Full Text Available This research evaluated the nutritive value of millet residue meal with fish meal, soybean meal or fish-soybean meal combination (1:2 as protein source. The treatments were labelled according to protein source as fish meal diet, soybean meal diet and fish-soybean meal diet. Thirty nulliparous mixed-breed rabbits of an initial mean (±SD body weight of 1852.6±122.7 g were used in a completely randomised design with 10 rabbits per treatment. The rabbits were allowed four weeks to adjust to the experimental diets before breeding was initiated. The feed intake during gestation and the reproductive data of the rabbits were assessed over two reproductive cycles. Does on the fish meal diet had poorer (P<0.05 DMI, daily growth rate and FCR than those on the soybean meal diet during the pre-breeding period. Does on the fish meal diet delivered less (P<0.05 number of kits at birth and weaned less kits compared to those on the soybean meal diet. Those on the soybean meal diet were heaviest (P<0.05 at mating and at kindling. They out-performed does on the fish meal diet in most of the parameters measured. Parity did not affect reproductive performance, but growth rate of the does had a positive linear relationship with DMI (r= 0.77; P<0.01 and a negative linear relationship with FCR (r= -0.83; P<0.01 during the pre-breeding period. It was concluded that millet residue meal may be a better feed source for rabbit does if soybean meal rather than fish meal or fish-soybean meal combination (1:2 is used to improve the protein content.

  12. Establishment of atherosclerotic model and USPIO enhanced MRI techniques study in rabbits

    International Nuclear Information System (INIS)

    Objective: To explore the methods of establishment of atherosclerotic model and USPIO enhanced MRI techniques in rabbits. Methods: Thirty New Zealand male rabbits were divided randomly into two groups: 20 animals in the experiment group, 10 animals in the control group. Animal model of atherosclerosis was induced with aortic balloon endothelial injury and high-fat diet feeding. There was no intervention with the rabbits in control group. MRI examination included plan scan, USPIO enhanced black-blood sequences and white-blood sequence. The features of the plaques was analyzed in the experimental group and the effection on the image quality of different coils, sequences and parameters and a statistical study was also analyzed. Results: Animal model of atherosclerosis was successfully made in 12 rabbits and most plaques located in the abdomen aorta. There were 86 plaques within the scanning scope among which 67 plaques were positive to the Prussian blue staining. The image quality of knee joint coil was better than that of other coils. Although there was no difference in the detection of numbers of AS plaques between USPIO enhanced black-blood sequences and white-blood sequence (P > 0.05), blackblood sequences was superior to white-blood sequence in the demonstration of the components of plaque. Conclusion: The method of aortic balloon endothelial injury and high-fat diet feeding can easily establish the AS model in rabbits with a shorter period and it may be used for controlling the location of the plaques. USPIO enhanced MRI sequences has high sensitivity in the detection of the AS plauqes and can reveal the component of AS plaques. The optimization of MRI techniques is very important in the improvement of the image quality and the detection of the plaques. (authors)

  13. Effects of Loud Noise on Oxidation and Lipid peroxidation Variations of Liver Tissue of Rabbit

    Directory of Open Access Journals (Sweden)

    Mirzaei Ramazan

    2009-06-01

    Full Text Available Background: In today's world, noise is one of the major physical pollutants. The exact mechanism leading to tissue damage in loud noise is not clear. There are increasing evidences that show damage to cochlear tissue by noise is linked to cell injury induced by free radical species. The aim of this study was to investigate the relationship between change in liver tissue glutathione (anti- oxidant and malondialdehyde (one metabolite of lipid oxidation levels that occur in rabbits which were exposed to continuous loud noise.Materials and Methods: This experimental study was performed on 12 white Newzeland male rabbits in Tarbiat Modarres University in 2004. The rabbits were assigned to the following two groups: control, and exposed to continuous loud noise for 96 hours (8 h/day for 12 days, SPL=110dBA and 250Hz to 20 KHz. The concentration of malondialdehyde (MDA and glutathione (GSH in liver tissue samples were measured in rabbits after exposure to noise. Thiobarbituric acid reacting substance, Ellman's reagent and spectrophotometry techniques were used for this measurement. The data were statically analyzed by SPSS software and 2 groups were compared by t-test. Differences at the level of P<0.05 were considered statistically significant.Results: Comparison of the biochemical parameters of GSH and MDA measured in treated group with control indicated that antioxidant and lipid peroxidants parameters were suppressed in treated group compared to control group (p<0.05.Conclusion: Possible similarities between rabbit and human biological system indicate the possible role of noise in causation of oxidative stress in context with liver tissue impairm

  14. ANTIOXIDANT EFFECT OF ALOE VERA IN EXPERIMENTALLY INDUCED DIABETES MELLITUS

    Directory of Open Access Journals (Sweden)

    Jyoti Sethi et al

    2012-08-01

    Full Text Available The aim of the present study was to evaluate antidiabetic and antioxidant potential of Aloe vera (AV in alloxan induced diabetes in albino rabbits. Experimental Diabetes was induced in rabbits with alloxan(80mg/Kg body weightand animals showing fasting blood glucose levels more than 250mg/dl were considered as diabetics and divided into four groups of six each (n=6. Group I: Normal control rabbits, Group II: Alloxan induced diabetic rabbits, Group III: Diabetic rabbits received AV gel extract (300 mg/Kg in aqueous solution for 21 days, Group IV: diabetic rabbits given glibenclamide (600ug/kg in aqueous solution. All the drugs were administered orally (using an intra gastric tube in a single dose in the morning for 21 days. Blood samples were collected from the marginal vein of pinna of overnight fasted rabbits (Blood sugar, glycosylated hemoglobin (HbA1c, Malondialdehyde (MDA, reduced glutathione (GSH, total thiols (PSH and Superoxide dismutase (SOD. Oral administration of AV showed potent antihyperglycemic and anti-lipidperoxidative effect in diabetic animals. Simultaneously, the levels of protective antioxidant enzymes (SOD, GSH and PSH were significantly increased with AV supplementation. The results suggest potent antidiabetic and antioxidant potential of AV in experimental diabetes, and thus Aloe vera can be used as an alternative remedy for treatment of diabetes mellitus and its complications.

  15. Dual energy CT ventilation imaging after aerosol inhalation of iodinated contrast medium in rabbits

    International Nuclear Information System (INIS)

    Purpose: To assess the feasibility of dual energy CT (DECT) after aerosol inhalation of iodinated contrast medium for the evaluation of ventilation function in rabbits with airway obstruction. Materials and methods: The study was approved by our institutional animal experimental committee and performed according to animal care guidelines. Airway obstruction was created by injecting gelatin sponge into the right bronchus of 6 New Zealand rabbits. One additional rabbit served as control without airway obstruction. All 7 rabbits then underwent inhalation of aerosol iodinated contrast medium for 5 min, followed by DECT of the lungs from which ventilation CT images were created. CT number and overlay value (calculated iodine enhancement on the ventilation images in hounsfield unit) of the obstructed and non-obstructed lung lobes were measured at 80-kVp, 140-kVp, and weighted average 120-kVp. Immediately after DECT scan, the rabbits were sacrificed, the lungs were removed and detailed pathological examination of the locations and parenchymal changes of the obstructed lung lobes were performed and correlated with DECT ventilation imaging findings. Results: Data from one rabbit with airway obstruction were excluded because of post-procedure pneumatothorax. Seventeen normal lung lobes without airway obstruction proven by histopathology had nearly homogeneous ventilation, while 13 abnormal lung lobes had ventilation defects on DECT v