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Sample records for caveolin-1 enhances fusion

  1. Blockade of CD26-mediated T cell costimulation with soluble caveolin-1-Ig fusion protein induces anergy in CD4+T cells

    International Nuclear Information System (INIS)

    CD26 binds to caveolin-1 in antigen-presenting cells (APC), and that ligation of CD26 by caveolin-1 induces T cell proliferation in a TCR/CD3-dependent manner. We report herein the effects of CD26-caveolin-1 costimulatory blockade by fusion protein caveolin-1-Ig (Cav-Ig). Soluble Cav-Ig inhibits T cell proliferation and cytokine production in response to recall antigen, or allogeneic APC. Our data hence suggest that blocking of CD26-associated signaling by soluble Cav-Ig may be an effective approach as immunosuppressive therapy.

  2. Caveolin-1 sensitizes rat pituitary adenoma GH3 cells to bromocriptine induced apoptosis

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    Huang Mu-Chiou

    2007-03-01

    Full Text Available Abstract Background Prolactinoma is the most frequent pituitary tumor in humans. The dopamine D2 receptor agonist bromocriptine has been widely used clinically to treat human breast tumor and prolactinoma through inhibition of hyperprolactinemia and induction of tumor cell apoptosis, respectively, but the molecular mechanism of bromocriptine induction of pituitary tumor apoptosis remains unclear. Caveolin-1 is a membrane-anchored protein enriched on caveolae, inverted flask-shaped invaginations on plasma membranes where signal transduction molecules are concentrated. Currently, caveolin-1 is thought to be a negative regulator of cellular proliferation and an enhancer of apoptosis by blocking signal transduction between cell surface membrane receptors and intracellular signaling protein cascades. Rat pituitary adenoma GH3 cells, which express endogenous caveolin-1, exhibit increased apoptosis and shrinkage after exposure to bromocriptine. Hence, the GH3 cell line is an ideal model for studying the molecular action of bromocriptine on prolactinoma. Results The expression of endogenous caveolin-1 in GH3 cells was elevated after bromocriptine treatment. Transiently expressed mouse recombinant caveolin-1 induced apoptosis in GH3 cells by enhancing the activity of caspase 8. Significantly, caveolin-1 induction of GH3 cell apoptosis was sensitized by the administration of bromocriptine. Phosphorylation of caveolin-1 at tyrosine 14 was enhanced after bromocriptine treatment, suggesting that bromocriptine-induced phosphorylation of caveolin-1 may contribute to sensitization of apoptosis in GH3 cells exposed to bromocriptine. Conclusion Our results reveal that caveolin-1 increases sensitivity for apoptosis induction in pituitary adenoma GH3 cells and may contribute to tumor shrinkage after clinical bromocriptine treatment.

  3. Calcium regulates caveolin-1 expression at the transcriptional level

    International Nuclear Information System (INIS)

    Highlights: ► Caveolin-1 expression is regulated by calcium signaling at the transcriptional level. ► An inhibitor of or siRNA to L-type calcium channel suppressed caveolin-1 expression. ► Cyclosporine A or an NFAT inhibitor markedly reduced caveolin-1 expression. ► Caveolin-1 regulation by calcium signaling is observed in several mouse cell lines. -- Abstract: Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca2+/calcineurin/NFAT.

  4. Combined loss of INK4a and caveolin-1 synergistically enhances cell proliferation and oncogene-induced tumorigenesis: role of INK4a/CAV-1 in mammary epithelial cell hyperplasia.

    Science.gov (United States)

    Williams, Terence M; Lee, Hyangkyu; Cheung, Michelle W-C; Cohen, Alex W; Razani, Babak; Iyengar, Puneeth; Scherer, Philipp E; Pestell, Richard G; Lisanti, Michael P

    2004-06-01

    Tumorigenesis is a multistep process that involves a series of genetic changes or "multiple hits," leading to alterations in signaling, proliferation, immortalization, and transformation. Many of the molecular factors that govern tumor initiation and progression remain unknown. Here, we evaluate the transformation suppressor potential of caveolin-1 (Cav-1) and its ability to cooperate with a well established tumor suppressor, the INK4a locus. To study the effects of loss of caveolin-1 on cellular transformation, we established immortalized primary mouse embryonic fibroblasts (MEFs) expressing and lacking caveolin-1 by interbreeding Cav-1 (+/+) and Cav-1 (-/-) mice with INK4a (-/-) mice. Analysis of these cells reveals that loss of caveolin-1 confers a significant growth advantage, as measured via cellular proliferation and cell cycle analysis. Loss of caveolin-1 in the INK4a (-/-) genetic background results in constitutive hyperactivation of the p42/44 MAP kinase cascade, decreased expression of p21(Cip1), as well as cyclin D1 and PCNA overexpression, consistent with their hyperproliferative phenotype. Importantly, in cells lacking Cav-1 expression, transformation by activated oncogenes (H-Ras(G12V) or v-Src) results in increased tumor growth in vivo (up to >40-fold). Finally, INK4a (-/-)/Cav-1 (-/-) mice demonstrate disturbed mammary epithelial ductal morphology, with hyperplasia, increased side-branching, and fibrosis. Our results provide important new evidence for the transformation suppressor properties of Cav-1 and the first molecular genetic evidence that Cav-1 cooperates with a tumor suppressor, namely the INK4a genetic locus. PMID:15044451

  5. Calcium regulates caveolin-1 expression at the transcriptional level

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    Yang, Xiao-Yan; Huang, Cheng-Cheng; Kan, Qi-Ming [Laboratory of Tumor Biology and Glycobiology, Department of Life Sciences, Shenyang Pharmaceutical University, Shenyang 110016, People' s Republic of China (China); Li, Yan [Experimental Animal Center, Department of Life Sciences, Shenyang Pharmaceutical University, Shenyang 110016, People' s Republic of China (China); Liu, Dan; Zhang, Xue-Cheng [Laboratory of Tumor Biology and Glycobiology, Department of Life Sciences, Shenyang Pharmaceutical University, Shenyang 110016, People' s Republic of China (China); Sato, Toshinori [Department of Biosciences and Informatics, Keio University, Hiyoshi, Yokohama 223-8522 (Japan); Yamagata, Sadako [Laboratory of Tumor Biology and Glycobiology, Department of Life Sciences, Shenyang Pharmaceutical University, Shenyang 110016, People' s Republic of China (China); Yamagata, Tatsuya, E-mail: tcyamagata@gmail.com [Laboratory of Tumor Biology and Glycobiology, Department of Life Sciences, Shenyang Pharmaceutical University, Shenyang 110016, People' s Republic of China (China)

    2012-09-28

    Highlights: Black-Right-Pointing-Pointer Caveolin-1 expression is regulated by calcium signaling at the transcriptional level. Black-Right-Pointing-Pointer An inhibitor of or siRNA to L-type calcium channel suppressed caveolin-1 expression. Black-Right-Pointing-Pointer Cyclosporine A or an NFAT inhibitor markedly reduced caveolin-1 expression. Black-Right-Pointing-Pointer Caveolin-1 regulation by calcium signaling is observed in several mouse cell lines. -- Abstract: Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca{sup 2+}/calcineurin/NFAT.

  6. Increased PEA3/E1AF and decreased Net/Elk-3, both ETS proteins, characterize human NSCLC progression and regulate caveolin-1 transcription in Calu-1 and NCI-H23 NSCLC cell lines

    OpenAIRE

    Sloan, Karin A.; Marquez, Hector A.; Li, Jun; Cao, Yuxia; Hinds, Anne; O'Hara, Carl J.; Kathuria, Satinder; Ramirez, Maria I.; Williams, Mary C.; Kathuria, Hasmeena

    2009-01-01

    Caveolin-1 protein has been called a ‘conditional tumor suppressor’ because it can either suppress or enhance tumor progression depending on cellular context. Caveolin-1 levels are dynamic in non-small-cell lung cancer, with increased levels in metastatic tumor cells. We have shown previously that transactivation of an erythroblastosis virus-transforming sequence (ETS) cis-element enhances caveolin-1 expression in a murine lung epithelial cell line. Based on high sequence homology between the...

  7. Escherichia coli K1 internalization via caveolae requires caveolin-1 and protein kinase Calpha interaction in human brain microvascular endothelial cells.

    Science.gov (United States)

    Sukumaran, Sunil K; Quon, Michael J; Prasadarao, Nemani V

    2002-12-27

    The morbidity and mortality associated with Escherichia coli K1 meningitis during the neonatal period have remained significant over the last decade and are once again on the rise. Transcytosis of brain microvascular endothelial cells (BMEC) by E. coli within an endosome to avoid lysosomal fusion is crucial for dissemination into the central nervous system. Central to E. coli internalization of BMEC is the expression of OmpA (outer membrane protein A), which interacts with its receptor for the actin reorganization that leads to invasion. However, nothing is known about the nature of the signaling events for the formation of endosomes containing E. coli K1. We show here that E. coli K1 infection of human BMEC (HBMEC) results in activation of caveolin-1 for bacterial uptake via caveolae. The interaction of caveolin-1 with phosphorylated protein kinase Calpha (PKCalpha) at the E. coli attachment site is critical for the invasion of HBMEC. Optical sectioning of confocal images of infected HBMEC indicates continuing association of caveolin-1 with E. coli during transcytosis. Overexpression of a dominant-negative form of caveolin-1 containing mutations in the scaffolding domain blocked the interaction of phospho-PKCalpha with caveolin-1 and the E. coli invasion of HBMEC, but not actin cytoskeleton rearrangement or the phosphorylation of PKCalpha. The interaction of caveolin-1 with phospho-PKCalpha was completely abrogated in HBMEC overexpressing dominant-negative forms of either focal adhesion kinase or PKCalpha. Treatment of HBMEC with a cell-permeable peptide that represents the scaffolding domain, which was coupled to an antennapedia motif of a Drosophila transcription factor significantly blocked the interaction of caveolin-1 with phospho-PKCalpha and E. coli invasion. These results show that E. coli K1 internalizes HBMEC via caveolae and that the scaffolding domain of caveolin-1 plays a significant role in the formation of endosomes. PMID:12386163

  8. Caveolar fatty acids and acylation of caveolin-1.

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    Qian Cai

    Full Text Available PURPOSE: Caveolae are cholesterol and sphingolipids rich subcellular domains on plasma membrane. Caveolae contain a variety of signaling proteins which provide platforms for signaling transduction. In addition to enriched with cholesterol and sphingolipids, caveolae also contain a variety of fatty acids. It has been well-established that acylation of protein plays a pivotal role in subcellular location including targeting to caveolae. However, the fatty acid compositions of caveolae and the type of acylation of caveolar proteins remain largely unknown. In this study, we investigated the fatty acids in caveolae and caveolin-1 bound fatty acids. METHODS: Caveolae were isolated from Chinese hamster ovary (CHO cells. The caveolar fatty acids were extracted with Folch reagent, methyl esterificated with BF3, and analyzed by gas chromatograph-mass spectrometer (GC/MS. The caveolin-1 bound fatty acids were immunoprecipitated by anti-caveolin-1 IgG and analyzed with GC/MS. RESULTS: In contrast to the whole CHO cell lysate which contained a variety of fatty acids, caveolae mainly contained three types of fatty acids, 0.48 µg palmitic acid, 0.61 µg stearic acid and 0.83 µg oleic acid/caveolae preparation/5 × 10(7 cells. Unexpectedly, GC/MS analysis indicated that caveolin-1 was not acylated by myristic acid; instead, it was acylated by palmitic acid and stearic acid. CONCLUSION: Caveolae contained a special set of fatty acids, highly enriched with saturated fatty acids, and caveolin-1 was acylated by palmitic acid and stearic acid. The unique fatty acid compositions of caveolae and acylation of caveolin-1 may be important for caveolae formation and for maintaining the function of caveolae.

  9. Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis.

    Science.gov (United States)

    Lee, Rebecca; Perry, Beth; Heywood, Jonathan; Reese, Charles; Bonner, Michael; Hatfield, Corey M; Silver, Richard M; Visconti, Richard P; Hoffman, Stanley; Tourkina, Elena

    2014-01-01

    In fibrotic diseases caveolin-1 underexpression in fibroblasts results in collagen overexpression and in monocytes leads to hypermigration. These profibrotic behaviors are blocked by the caveolin-1 scaffolding domain peptide (CSD) which compensates for caveolin-1 deficiency. Monocytes and fibroblasts are related in that monocytes are the progenitors of fibrocytes (CD45+/Collagen I+ cells) that, in turn, are the progenitors of many fibroblasts in fibrotic tissues. In an additional anti-fibrotic activity, CSD blocks monocyte differentiation into fibrocytes. We studied a mouse fibrosis model (Pump Model) involving systemic bleomycin delivery that closely models scleroderma (SSc) in several ways, the most important of which for this study is that fibrosis is observed in the lungs, skin, and internal organs. We show here that dermal thickness is increased 2-fold in the Pump Model and that this effect is almost completely blocked by CSD (p 80% thinner. This effect is also blocked by CSD (p Fibrocytes and other leukocytes expressing CCR5 and its ligands were present at high levels in the fibrotic dermis of SSc patients and Pump Model mice while CSD blocked their accumulation in mouse dermis. Migration toward CCR5 ligands of SSc monocytes and Pump Model bone marrow cells was 3-fold greater than cells from control subjects. This enhanced migration was almost completely blocked by CSD. These results suggest that low monocyte caveolin-1 promotes fibrosis by enhancing the recruitment of fibrocytes and their progenitors into affected tissue. PMID:24966836

  10. Caveolin-1 Up-regulation during Epithelial to Mesenchymal Transition Is Mediated by Focal Adhesion Kinase*

    OpenAIRE

    Bailey, Kelly M.; Liu, Jun

    2008-01-01

    Emerging evidence has shown that caveolin-1 is up-regulated in a number of metastatic cancers and can influence various aspects of cell migration. However, in general, the role of caveolin-1 in cancer progression is poorly understood. In the present study, we examined alterations in caveolin-1 expression during epithelial-to-mesenchymal transition (EMT) and the ability of caveolin-1 to alter cancer cell adhesion, an aspect of cell motility. We employed two EMT cell models, the human embryonic...

  11. Enhanced image capture through fusion

    Science.gov (United States)

    Burt, Peter J.; Hanna, Keith; Kolczynski, Raymond J.

    1993-01-01

    Image fusion may be used to combine images from different sensors, such as IR and visible cameras, to obtain a single composite with extended information content. Fusion may also be used to combine multiple images from a given sensor to form a composite image in which information of interest is enhanced. We present a general method for performing image fusion and show that this method is effective for diverse fusion applications. We suggest that fusion may provide a powerful tool for enhanced image capture with broad utility in image processing and computer vision.

  12. Caveolin-1 Protects B6129 Mice against Helicobacter pylori Gastritis

    Science.gov (United States)

    Hitkova, Ivana; Yuan, Gang; Anderl, Florian; Gerhard, Markus; Kirchner, Thomas; Reu, Simone; Röcken, Christoph; Schäfer, Claus; Schmid, Roland M.; Vogelmann, Roger; Ebert, Matthias P. A.; Burgermeister, Elke

    2013-01-01

    Caveolin-1 (Cav1) is a scaffold protein and pathogen receptor in the mucosa of the gastrointestinal tract. Chronic infection of gastric epithelial cells by Helicobacter pylori (H. pylori) is a major risk factor for human gastric cancer (GC) where Cav1 is frequently down-regulated. However, the function of Cav1 in H. pylori infection and pathogenesis of GC remained unknown. We show here that Cav1-deficient mice, infected for 11 months with the CagA-delivery deficient H. pylori strain SS1, developed more severe gastritis and tissue damage, including loss of parietal cells and foveolar hyperplasia, and displayed lower colonisation of the gastric mucosa than wild-type B6129 littermates. Cav1-null mice showed enhanced infiltration of macrophages and B-cells and secretion of chemokines (RANTES) but had reduced levels of CD25+ regulatory T-cells. Cav1-deficient human GC cells (AGS), infected with the CagA-delivery proficient H. pylori strain G27, were more sensitive to CagA-related cytoskeletal stress morphologies (“humming bird”) compared to AGS cells stably transfected with Cav1 (AGS/Cav1). Infection of AGS/Cav1 cells triggered the recruitment of p120 RhoGTPase-activating protein/deleted in liver cancer-1 (p120RhoGAP/DLC1) to Cav1 and counteracted CagA-induced cytoskeletal rearrangements. In human GC cell lines (MKN45, N87) and mouse stomach tissue, H. pylori down-regulated endogenous expression of Cav1 independently of CagA. Mechanistically, H. pylori activated sterol-responsive element-binding protein-1 (SREBP1) to repress transcription of the human Cav1 gene from sterol-responsive elements (SREs) in the proximal Cav1 promoter. These data suggested a protective role of Cav1 against H. pylori-induced inflammation and tissue damage. We propose that H. pylori exploits down-regulation of Cav1 to subvert the host's immune response and to promote signalling of its virulence factors in host cells. PMID:23592983

  13. Distinct Roles of Endothelial and Adipocyte Caveolin-1 in Macrophage Infiltration and Adipose Tissue Metabolic Activity

    OpenAIRE

    Briand, N.; Le Lay, S.; Sessa, W. C.; Ferre, P.; Dugail, I.

    2011-01-01

    OBJECTIVE Defective caveolin-1 expression is now recognized as a cause of lipoatrophic diabetes in patients, due to primary caveolin gene mutations or secondary caveolin deficiency caused by PTRF/cavin gene defects. The goal of this study was to establish the relative contribution of endothelial cells and adipocytes, both highly expressing caveolin-1 to the lipoatrophic phenotype of mice with global caveolin-1 gene invalidation (Cav1-KO). RESEARCH DESIGN AND METHODS We compared adipose tissue...

  14. Caveolin-1 expression in benign and malignant lesions of the breast

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    Kiesel Ludwig

    2007-10-01

    Full Text Available Abstract Background Caveolin-1 is thought to have an important impact on both signal transduction and mediation of intracellular processes. Furthermore, it has been suggested that Caveolin-1 may contribute to certain steps of carcinogenesis in various types of cancer. We examined the potential clinical relevance of Caveolin-1 in normal, benign and malignant breast tissue specimens. Methods Using tissue microarray (TMA technology cases of invasive breast cancer, DCIS, benign breast disease (i.e. fibroadenoma, sclerosing adenosis, ductal hyperplasia and radial scar and normal breast tissue were evaluated for Caveolin-1 expression. Immunohistochemical staining with an anti-Caveolin-1-antibody was performed. Staining intensity was quantified semiquantitatively. In invasive lesions staining results were correlated with clinical and pathological data. Results No Caveolin-1 expression was observed in epithelial cells of normal breast tissue (n = 5, benign breast disease (n = 295 and DCIS (n = 108. However, Caveolin-1 expression was found in 32 of 109 cases of invasive breast carcinomas (29.4%. Caveolin-1 expression in invasive breast cancer could neither be correlated with survival parameters such as overall or disease-free survival nor with established clinical and pathological markers. Conclusion In this study we demonstrated expression of Caveolin-1 in one third of invasive breast cancers. A significant increase in Caveolin-1 expression was observed comparing invasive breast cancer to both benign breast tissue and non-invasive breast cancer. Since inhibitors of Caveolin-1 signalling are available, targeting Caveolin-1 in breast cancer may represent a potential option for future breast cancer treatment.

  15. Caveolin-1 expression in benign and malignant lesions of the breast

    OpenAIRE

    Kiesel Ludwig; Bürger Horst; Kersting Christian; Liedtke Cornelia; Wülfing Pia

    2007-01-01

    Abstract Background Caveolin-1 is thought to have an important impact on both signal transduction and mediation of intracellular processes. Furthermore, it has been suggested that Caveolin-1 may contribute to certain steps of carcinogenesis in various types of cancer. We examined the potential clinical relevance of Caveolin-1 in normal, benign and malignant breast tissue specimens. Methods Using tissue microarray (TMA) technology cases of invasive breast cancer, DCIS, benign breast disease (i...

  16. Caveolin-1 Expression in Different Types of Psoriatic Lesions: Analysis of 66 Cases

    OpenAIRE

    Feng Zhang; Heyu Li; Yicheng Zhou; Yunhe Gu; Lifeng Wang

    2014-01-01

    Background: Caveolin-1 is a key structural and functional protein. Caveolin-1 is known to modulate multiple signal-transducing pathways involved in cell differentiation and proliferation. Psoriasis is viewed as a multifactorial pathology characterized by keratinocyte hyperproliferation and abnormal cell maturation. Objectives: To examine the expression of caveolin-1 in skin biopsies from normal subjects, patients, and subjects with the three respective isoforms of psoriasis (psoriasis vulgari...

  17. Caveolin-1 Protects B6129 Mice against Helicobacter pylori Gastritis

    OpenAIRE

    Hitkova, Ivana; Yuan, Gang; Anderl, Florian; Gerhard, Markus; Kirchner, Thomas; Reu, Simone; Röcken, Christoph; Schäfer, Claus; Schmid, Roland M; Vogelmann, Roger; Ebert, Matthias P A; Burgermeister, Elke

    2013-01-01

    Caveolin-1 (Cav1) is a scaffold protein and pathogen receptor in the mucosa of the gastrointestinal tract. Chronic infection of gastric epithelial cells by Helicobacter pylori (H. pylori) is a major risk factor for human gastric cancer (GC) where Cav1 is frequently down-regulated. However, the function of Cav1 in H. pylori infection and pathogenesis of GC remained unknown. We show here that Cav1-deficient mice, infected for 11 months with the CagA-delivery deficient H. pylori strain SS1, deve...

  18. Hepatitis B virus X protein suppresses caveolin-1 expression in hepatocellular carcinoma by regulating DNA methylation

    International Nuclear Information System (INIS)

    To understand the molecular mechanisms of caveolin-1 downregulation by hepatitis B virus X protein (HBx). The DNA methylation status of the caveolin-1 promoter was examined by nested methylation-specific PCR of 33 hepatitis B virus (HBV)-infected hepatocellular carcinoma (HCC) samples. The SMMC-7721 hepatoma cell line was transfected with a recombinant HBx adenoviral vector, and the effects of HBx protein on caveolin-1 expression and promoter methylation were examined and confirmed by sequencing. A reporter gene containing the caveolin-1 promoter region was constructed, and the effects of HBx on the transcriptional activity of the promoter were also studied. Methylation of the caveolin-1 promoter was detected in 84.8% (28/33) of HBV-infected HCC samples. Expression of caveolin-1 was significantly downregulated (P = 0.022), and multiple CpG sites in the promoter region of caveolin-1 were methylated in SMMC-7721 cells after HBx transfection. Transfected HBx significantly suppressed caveolin-1 promoter activity (P = 0.001). HBx protein induces methylation of the caveolin-1 promoter region and suppresses its expression

  19. Caveolin-1 Regulates Chemokine Receptor 5-Mediated Contribution of Bone Marrow-Derived Cells to Dermal Fibrosis

    Directory of Open Access Journals (Sweden)

    ElenaTourkina

    2014-06-01

    Full Text Available In fibrotic diseases caveolin-1 underexpression in fibroblasts results in collagen overexpression and in monocytes leads to hypermigration. These profibrotic behaviors are blocked by the caveolin-1 scaffolding domain peptide (CSD which compensates for caveolin-1 deficiency. Monocytes and fibroblasts are related in that monocytes are the progenitors of fibrocytes (CD45+/Collagen I+ cells that, in turn, are the progenitors of many fibroblasts in fibrotic tissues. In an additional anti-fibrotic activity, CSD blocks monocyte differentiation into fibrocytes. We studied a mouse fibrosis model (Pump Model involving systemic bleomycin delivery that closely models scleroderma (SSc in several ways, the most important of which for this study is that fibrosis is observed in the lungs, skin, and internal organs. We show here that dermal thickness is increased 2-fold in the Pump Model and that this effect is almost completely blocked by CSD (p 80 % thinner. This effect is also blocked by CSD (p < 0.001. Even in mice receiving vehicle instead of bleomycin, CSD increases the thickness of the fat layer. To study the mechanisms of action of bleomycin and CSD, we examined the accumulation of the chemokine receptor CCR5 and its ligands MIP1α and MIP1β in fibrotic tissue and their roles in monocyte migration. Fibrocytes and other leukocytes expressing CCR5 and its ligands were present at high levels in the fibrotic dermis of SSc patients and Pump Model mice while CSD blocked their accumulation in mouse dermis. Migration toward CCR5 ligands of SSc monocytes and Pump Model bone marrow cells was 3-fold greater than cells from control subjects. This enhanced migration was almost completely blocked by CSD. These results suggest that low monocyte caveolin-1 promotes fibrosis by enhancing the recruitment of fibrocytes and their progenitors into affected tissue.

  20. Caveolin-1 overexpression in benign and malignant salivary gland tumors.

    Science.gov (United States)

    Jaafari-Ashkavandi, Zohreh; Ashraf, Mohammad Javad; Nazhvani, Ali Dehghani; Azizi, Zahra

    2016-02-01

    Caveolin-1, a tyrosine-phosphorylated protein, is supposed to have different regulatory roles as promoter or suppressor in many human cancers. However, no published study concerned its expression in benign and malignant salivary gland tumors. The aim of this study was to evaluate and compare the expression of Cav-1 in the most common benign and malignant salivary gland tumors and evaluate its correlation with proliferation activity. In this cross-sectional retrospective study, immunohistochemical expression of caveolin-1 and Ki67 were evaluated in 49 samples, including 11 normal salivary glands, 15 cases of pleomorphic adenoma (PA), 13 adenoid cystic carcinomas (AdCC), and 10 mucoepidermoid carcinomas (MEC). The expression of Cav-1 was seen in 18 % of normal salivary glands and 85 % of tumors. The immunoreaction in the tumors was significantly higher than normal tissues (P = 0.001), but the difference between benign and malignant tumors was not significant (P = 0.07). Expression of Cav-1 was correlated with Ki67 labeling index in PAs, but not in malignant tumors. Cav-1 expression was not in association with tumor size and stage. Overexpression of Cav-1 was found in salivary gland tumors in comparison with normal tissues, but no significant difference was observed between benign and malignant tumors. Cav-1 was inversely correlated with proliferation in PA. Therefore, this marker may participate in tumorigenesis of salivary gland tumors and may be a potential biomarker for cancer treatments. PMID:26323261

  1. Spatiotemporal expression of caveolin-1 and EMMPRIN during mouse tooth development.

    Science.gov (United States)

    Shi, Lu; Li, Lingyun; Wang, Ding; Li, Shu; Chen, Zhi; An, Zhengwen

    2016-06-01

    Caveolin-1 is a scaffolding protein involved in the formation of cholesterol-rich caveolae lipid rafts within the plasma membrane and is capable of collecting signaling molecules into the caveolae and regulating their activity, including extracellular matrix metalloproteinase inducer (EMMPRIN). However, detailed expression patterns of caveolin-1 and EMMPRIN in the developing dental germ are largely unknown. The present study investigated the expression patterns of caveolin-1 and EMMPRIN in the developing mouse tooth germ by immunohistochemistry and real-time polymerase chain reaction. At the bud stage, caveolin-1 expression was initiated in the epithelium bud and mesenchymal cells, while EMMPRIN was weakly expressed at this stage. At the cap stage, caveolin-1 protein was located in the lingual part of the tooth germ; however, EMMPRIN protein was located in the labial part. From the bell stage to 2 days postnatal, caveolin-1 expression was detected in the ameloblasts and cervical loop area; with EMMPRIN expression in the ameloblasts and odontoblasts. Real-time polymerase chain reaction results showed that both caveolin-1 and EMMPRIN mRNA levels increased gradually with progression of developmental stages, and peaked at day two postnatal. The current finding suggests that both caveolin-1 and EMMPRIN take part in mouse tooth development, especially in the differentiation and organization of odontogenic tissues. PMID:27075451

  2. Caveolae and Caveolin-1 Integrate Reverse Cholesterol Transport and Inflammation in Atherosclerosis.

    Science.gov (United States)

    Qin, Li; Zhu, Neng; Ao, Bao-Xue; Liu, Chan; Shi, Ya-Ning; Du, Ke; Chen, Jian-Xiong; Zheng, Xi-Long; Liao, Duan-Fang

    2016-01-01

    Lipid disorder and inflammation play critical roles in the development of atherosclerosis. Reverse cholesterol transport is a key event in lipid metabolism. Caveolae and caveolin-1 are in the center stage of cholesterol transportation and inflammation in macrophages. Here, we propose that reverse cholesterol transport and inflammation in atherosclerosis can be integrated by caveolae and caveolin-1. PMID:27011179

  3. Caveolae and Caveolin-1 Integrate Reverse Cholesterol Transport and Inflammation in Atherosclerosis

    OpenAIRE

    Li Qin; Neng Zhu; Bao-Xue Ao; Chan Liu; Ya-Ning Shi; Ke Du; Jian-Xiong Chen; Xi-Long Zheng; Duan-Fang Liao

    2016-01-01

    Lipid disorder and inflammation play critical roles in the development of atherosclerosis. Reverse cholesterol transport is a key event in lipid metabolism. Caveolae and caveolin-1 are in the center stage of cholesterol transportation and inflammation in macrophages. Here, we propose that reverse cholesterol transport and inflammation in atherosclerosis can be integrated by caveolae and caveolin-1.

  4. Caveolae and Caveolin-1 Integrate Reverse Cholesterol Transport and Inflammation in Atherosclerosis

    Directory of Open Access Journals (Sweden)

    Li Qin

    2016-03-01

    Full Text Available Lipid disorder and inflammation play critical roles in the development of atherosclerosis. Reverse cholesterol transport is a key event in lipid metabolism. Caveolae and caveolin-1 are in the center stage of cholesterol transportation and inflammation in macrophages. Here, we propose that reverse cholesterol transport and inflammation in atherosclerosis can be integrated by caveolae and caveolin-1.

  5. Caveolin-1 Is Necessary for Hepatic Oxidative Lipid Metabolism: Evidence for Crosstalk between Caveolin-1 and Bile Acid Signaling

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    Manuel A. Fernández-Rojo

    2013-07-01

    Full Text Available Caveolae and caveolin-1 (CAV1 have been linked to several cellular functions. However, a model explaining their roles in mammalian tissues in vivo is lacking. Unbiased expression profiling in several tissues and cell types identified lipid metabolism as the main target affected by CAV1 deficiency. CAV1−/− mice exhibited impaired hepatic peroxisome proliferator-activated receptor α (PPARα-dependent oxidative fatty acid metabolism and ketogenesis. Similar results were recapitulated in CAV1-deficient AML12 hepatocytes, suggesting at least a partial cell-autonomous role of hepatocyte CAV1 in metabolic adaptation to fasting. Finally, our experiments suggest that the hepatic phenotypes observed in CAV1−/− mice involve impaired PPARα ligand signaling and attenuated bile acid and FXRα signaling. These results demonstrate the significance of CAV1 in (1 hepatic lipid homeostasis and (2 nuclear hormone receptor (PPARα, FXRα, and SHP and bile acid signaling.

  6. Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability

    International Nuclear Information System (INIS)

    Caveolin-1 is the major component protein of caveolae and associated with a lot of cellular events such as endocytosis, cholesterol homeostasis, signal transduction, and tumorigenesis. The majority of results suggest that caveolin-1 might not only act as a tumor suppressor gene but also a promoting metastasis gene. In this study, the divergent expression and roles of caveolin-1 were investigated in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepa1-6, which have high, low, and no metastatic potential in the lymph nodes, as compared with normal mouse liver cell line IAR-20. The results showed that expression of caveolin-1 mRNA and protein along with the amount of caveolae number in Hca-F cells was higher than that in Hca-P cells, but was not detectable in Hepa1-6 cells. When caveolin-1 expression in Hca-F cells was down-regulated by RNAi approach, Hca-F cells proliferation rate in vitro declined and the expression of lymphangiogenic factor VEGFA in Hca-F decreased as well. Furthermore, in vivo implantation assay indicated that reduction of caveolin-1 expression in Hca-F prevented the lymphatic metastasis tumor burden of Hca-F cells in 615 mice. These results suggest that caveolin-1 facilities the lymphatic metastasis ability of mouse hepatocarcinoma cells via regulation tumor cell growth and VEGFA expression

  7. Downregulation of caveolin-1 contributes to the synaptic plasticity deficit in the hippocampus of aged rats*******

    Institute of Scientific and Technical Information of China (English)

    Yang Liu; Zhanhua Liang; Jing Liu; Wei Zou; Xiaoyan Li; Yachen Wang; Lijia An

    2013-01-01

    Caveolin-1 is involved in the regulation of synaptic plasticity, but the relationship between its pression and cognitive function during aging remains controversial. To explore the relationship be-tween synaptic plasticity in the aging process and changes in learning and memory, we examined caveolin-1 expression in the hippocampus, cortex and cerebel um of rats at different ages. We also examined the relationship between the expression of caveolin-1 and synaptophysin, a marker of synaptic plasticity. Hippocampal caveolin-1 and synaptophysin expression in aged (22-24 month old) rats was significantly lower than that in young (1 month old) and adult (4 months old) rats. pression levels of both proteins were significantly greater in the cortex of aged rats than in that of young or adult rats, and levels were similar between the three age groups in the cerebel um. Linear regression analysis revealed that hippocampal expression of synaptophysin was associated with memory and learning abilities. Moreover, synaptophysin expression correlated positively with caveolin-1 expression in the hippocampus, cortex and cerebel um. These results confirm that caveolin-1 has a regulatory effect on synaptic plasticity, and suggest that the downregulation of hippocampal caveolin-1 expression causes a decrease in synaptic plasticity during physiological aging.

  8. Deletion of Caveolin-1 Protects against Oxidative Lung Injury via Up-Regulation of Heme Oxygenase-1

    Science.gov (United States)

    Jin, Yang; Kim, Hong Pyo; Chi, Minli; Ifedigbo, Emeka; Ryter, Stefan W.; Choi, Augustine M. K.

    2008-01-01

    Acute lung injury (ALI) is a major cause of morbidity and mortality in critically ill patients. Hyperoxia causes lung injury in animals and humans, and is an established model of ALI. Caveolin-1, a major constituent of caveolae, regulates numerous biological processes, including cell death and proliferation. Here we demonstrate that caveolin-1–null mice (cav-1−/−) were resistant to hyperoxia-induced death and lung injury. Cav-1−/− mice sustained reduced lung injury after hyperoxia as determined by protein levels in bronchoalveolar lavage fluid and histologic analysis. Furthermore, cav-1−/− fibroblasts and endothelial cells and cav-1 knockdown epithelial cells resisted hyperoxia-induced cell death in vitro. Basal and inducible expression of the stress protein heme oxygenase-1 (HO-1) were markedly elevated in lung tissue or fibroblasts from cav-1−/− mice. Hyperoxia induced the physical interaction between cav-1 and HO-1 in fibroblasts assessed by co-immunoprecipitation studies, which resulted in attenuation of HO activity. Inhibition of HO activity with tin protoporphyrin-IX abolished the survival benefits of cav-1−/− cells and cav-1−/− mice exposed to hyperoxia. The cav-1−/− mice displayed elevated phospho-p38 mitogen-activated protein kinase (MAPK) and p38β expression in lung tissue/cells under basal conditions and during hyperoxia. Treatment with SB202190, an inhibitor of p38 MAPK, decreased hyperoxia-inducible HO-1 expression in wild-type and cav-1−/− fibroblasts. Taken together, our data demonstrated that cav-1 deletion protects against hyperoxia-induced lung injury, involving in part the modulation of the HO-1–cav-1 interaction, and the enhanced induction of HO-1 through a p38 MAPK–mediated pathway. These studies identify caveolin-1 as a novel component involved in hyperoxia-induced lung injury. PMID:18323531

  9. Correlation of caveolin-1 expression with microlymphatic vessel density in colorectal adenocarcinoma tissues and its correlation with prognosis

    Institute of Scientific and Technical Information of China (English)

    Jun; Xue; Xue-Liang; Wu; Xian-Tao; Huang; Fei; Guo; Hong-Feng; Yu; Peng-Cheng; Zhang; Li-Kun; Wang; Ming; Qu; Li-Ming; Pan

    2015-01-01

    Objective: To study the expression of caveolin-1 in colorectal adenocarcinoma tissues and its correlation with microlymphatic vessel density(LMVD), and to investigate the clinical pathological prognostic significance of caveolin-1 and LMVD in patients with colorectal cancer. Methods: The expression of caveolin-1 and LMVD in 45 specimens of normal colorectal tissues, and 90 specimens of colorectal adenocarcinoma tissues were detected by immunohistochemistry technique. The correlation between their expression and the clinicopathologic features was analyzed. Muhivariable Cox regression was used to analyze the association between the laboratory indices and overall survival time. Results: The positive rates of caveolin-1 in colorectal adenocarcinoma tissues were significantly higher than those in normal colorectal tissues(P<0.01). LMVD in colorectal adenocarcinoma tissues were significantly higher than those in normal colorectal tissues(P<0.01). Mean LMVD in group with caveolin-1 positive was significantly higher than in that with caveolin-1 negative. The median survival time was 26.7 months. Cox regression analysis showed that the caveolin-1 expression, invation depth, lymph nodemetastasis, TNM stage, liver metastasis and LMVD were independent risk factors of overall survival time of patients with colorectal carcinoma. Conclusions: Caveolin-1 may contribute to the lymphangiogenesis in the tumor. During the occurrence and development of colorectal adenocarcinoma, there is a close relationship between the expression of caveolin-1 and lymphatic microvessel of tumor. Caveolin-1 expression and microlymphatic vessel density are significant prognostic value of colorectal carcinoma.

  10. Potential role of caveolin-1 in acetaminophen-induced hepatotoxicity

    International Nuclear Information System (INIS)

    Caveolin-1 (Cav-1) is a membrane scaffolding protein, which functions to regulate intracellular compartmentalization of various signaling molecules. In the present studies, transgenic mice with a targeted disruption of the Cav-1 gene (Cav-1-/-) were used to assess the role of Cav-1 in acetaminophen-induced hepatotoxicity. Treatment of wild-type mice with acetaminophen (300 mg/kg) resulted in centrilobular hepatic necrosis and increases in serum transaminases. This was correlated with decreased expression of Cav-1 in the liver. Acetaminophen-induced hepatotoxicity was significantly attenuated in Cav-1-/- mice, an effect that was independent of acetaminophen metabolism. Acetaminophen administration resulted in increased hepatic expression of the oxidative stress marker, lipocalin 24p3, as well as hemeoxygenase-1, but decreased glutathione and superoxide dismutase-1; no differences were noted between the genotypes suggesting that reduced toxicity in Cav-1-/- mice is not due to alterations in antioxidant defense. In wild-type mice, acetaminophen increased mRNA expression of the pro-inflammatory cytokines, interleukin-1β, and monocyte chemoattractant protein-1 (MCP-1), as well as cyclooxygenase-2, while 15-lipoxygenase (15-LOX), which generates anti-inflammatory lipoxins, decreased. Acetaminophen-induced changes in MCP-1 and 15-LOX expression were greater in Cav-1-/- mice. Although expression of tumor necrosis factor-α, a potent hepatocyte mitogen, was up-regulated in the liver of Cav-1-/- mice after acetaminophen, expression of proliferating cell nuclear antigen and survivin, markers of cellular proliferation, were delayed, which may reflect the reduced need for tissue repair. Taken together, these data demonstrate that Cav-1 plays a role in promoting inflammation and toxicity during the pathogenesis of acetaminophen-induced injury.

  11. Identification of a novel prostate cancer biomarker, caveolin-1: Implications and potential clinical benefit

    International Nuclear Information System (INIS)

    While prostate cancer is a common disease in men, it is uncommonly life-threatening. To better understand this phenomenon, tumor biologists have sought to elucidate the mechanisms that contribute to the development of virulent prostate cancer. The recent discovery that caveolin-1 (Cav-1) functions as an important oncogene involved in prostate cancer progression reflects the success of this effort. Cav-1 is a major structural coat protein of caveolae, specialized plasma membrane invaginations involved in multiple cellular functions, including molecular transport, cell adhesion, and signal transduction. Cav-1 is aberrantly overexpressed in human prostate cancer, with higher levels evident in metastatic versus primary sites. Intracellular Cav-1 promotes cell survival through activation of Akt and enhancement of additional growth factor pro-survival pathways. Cav-1 is also secreted as a biologically active molecule that promotes cell survival and angiogenesis within the tumor microenvironment. Secreted Cav-1 can be reproducibly detected in peripheral blood using a sensitive and specific immunoassay. Cav-1 levels distinguish men with prostate cancer from normal controls, and preoperative Cav-1 levels predict which patients are at highest risk for relapse following radical prostatectomy for localized disease. Thus, secreted Cav-1 is a promising biomarker in identifying clinically significant prostate cancer

  12. The Impact of Simulated Weightlessness on Endothelium-Dependent Angiogenesis and the Role of Caveolae/Caveolin-1

    Directory of Open Access Journals (Sweden)

    Fei Shi

    2016-02-01

    Full Text Available Background/Aims: The potential role of caveolin-1 in modulating angiogenesis in microgravity environment is unexplored. Methods: Using simulated microgravity by clinostat, we measured the expressions and interactions of caveolin-1 and eNOS in human umbilical vein endothelial cells. Results: We found that decreased caveolin-1 expression is associated with increased expression and phosphorylation levels of eNOS in endothelial cells stimulated by microgravity, which causes a dissociation of eNOS from caveolin-1 complexes. As a result, microgravity induces cell migration and tube formation in endothelial cell in vitro that depends on the regulations of caveolin-1. Conclusion: Our study provides insight for the important endothelial functions in altered gravitational environments.

  13. Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.

    Directory of Open Access Journals (Sweden)

    Michelle M Hill

    Full Text Available Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect of PTRF expression on cell migration. Using fluorescence imaging, quantitative proteomics, and cell migration assays we show that PTRF/cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCα caveola recruitment. PTRF/cavin-1 quantitatively reduced cell migration, and induced mesenchymal epithelial reversion. Similar to caveolin-1, the polarization of PTRF/cavin-1 was dependent on the migration mode. By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration.

  14. Effects of Raloxifene on Caveolin-1 mRNA and Protein Expressions in Vascular Smooth Muscle Cells

    Institute of Scientific and Technical Information of China (English)

    Fa-Lin YANG; Hong HE; Xian-Xi LIU; Bing TU; Xian-Wei ZENG; Ji-Xin SU; Xin WANG; Qin HU

    2006-01-01

    Caveolin-1 is regulated by estrogen in vascular smooth muscle cells. Raloxifene, a selectiveestrogen receptor modulator that possibly has cardioprotective properties without an increased risk of c ancer or other side effects of estrogen, may be used in women with risk of coronary artery disease. However, the relationship between raloxifene and caveolin-1 is still unknown. Therefore, this study was designed to see whether raloxifene regulates caveolin- 1 expression and if so, whether such regulation is mediated by estrogen receptor. Rat aortic smooth muscle cells were cultured in the absence or presence of raloxifene (10-8 to 10-6 M) for 12 or 24 h. Both mRNA and protein levels of caveolin-1 were increased significantly after 24 h treatment with raloxifene. These increases were inhibited by estrogen receptor antagonist ICI 182780 (10-5 M). Results of this study suggest that raloxifene stimulates caveolin- 1 transcription and translation through estrogen receptor mediated mechanisms.

  15. Pulmonary hypertension and metabolic syndrome: Possible connection, PPARγ and Caveolin-1

    Institute of Scientific and Technical Information of China (English)

    Rajamma; Mathew

    2014-01-01

    A number of disparate diseases can lead to pulmonary hypertension(PH), a serious disorder with a high morbidity and mortality rate. Recent studies suggest that the associated metabolic dysregulation may be an important factor adversely impacting the prognosis of PH. Furthermore, metabolic syndrome is associated with vascular diseases including PH. Inflammation plays a significant role both in PH and metabolic syndrome. Adipose tissue modulates lipid and glucose metabolism, and also produces pro-and anti-inflammatory adipokines that modulate vascular function and angiogenesis, suggesting a close functional relationship between the adipose tissue and the vasculature. Both caveolin-1, a cell membrane scaffolding protein and peroxisome proliferator-activated receptor(PPAR) γ, a ligandactivated transcription factor are abundantly expressed in the endothelial cells and adipocytes. Both caveolin-1 and PPARγ modulate proliferative and anti-apoptotic pathways, cell migration, inflammation, vascular homeostasis, and participate in lipid transport, triacylglyceride synthesis and glucose metabolism. Caveolin-1 and PPARγ regulate the production of adipokines and in turn are modulated by them. This review article summarizes the roles and inter-relationships of caveolin-1,PPARγ and adipokines in PH and metabolic syndrome.

  16. Oxidative stress induces caveolin 1 degradation and impairs caveolae functions in skeletal muscle cells.

    Directory of Open Access Journals (Sweden)

    Alexis Mougeolle

    Full Text Available Increased level of oxidative stress, a major actor of cellular aging, impairs the regenerative capacity of skeletal muscle and leads to the reduction in the number and size of muscle fibers causing sarcopenia. Caveolin 1 is the major component of caveolae, small membrane invaginations involved in signaling and endocytic trafficking. Their role has recently expanded to mechanosensing and to the regulation of oxidative stress-induced pathways. Here, we increased the amount of reactive oxidative species in myoblasts by addition of hydrogen peroxide (H2O2 at non-toxic concentrations. The expression level of caveolin 1 was significantly decreased as early as 10 min after 500 μM H2O2 treatment. This reduction was not observed in the presence of a proteasome inhibitor, suggesting that caveolin 1 was rapidly degraded by the proteasome. In spite of caveolin 1 decrease, caveolae were still able to assemble at the plasma membrane. Their functions however were significantly perturbed by oxidative stress. Endocytosis of a ceramide analog monitored by flow cytometry was significantly diminished after H2O2 treatment, indicating that oxidative stress impaired its selective internalization via caveolae. The contribution of caveolae to the plasma membrane reservoir has been monitored after osmotic cell swelling. H2O2 treatment increased membrane fragility revealing that treated cells were more sensitive to an acute mechanical stress. Altogether, our results indicate that H2O2 decreased caveolin 1 expression and impaired caveolae functions. These data give new insights on age-related deficiencies in skeletal muscle.

  17. Fusion Enhanced Vehicle Level Diagnostic System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Global Technology Connection, Inc. in conjunction with its partner, Vanderbilt University, is proposing to build a Fusion-enhanced Vehicle Diagnostics System (FVDS)...

  18. Caveolin-1, E-cadherin and β-catenin in Gastric Carcinoma, Precancerous Tissues and Chronic Non-atrophic Gastritis

    Institute of Scientific and Technical Information of China (English)

    Guo-yang Sun; Jun-xia Wu; Jian-sheng Wu; Yu-ting Pan; Rong Jin

    2012-01-01

    Objective:To investigate the expressions of caveolin-1,E-cadherin and β-catenin in gastric carcinoma,precancerous gastric and chronic non-atrophic gastritis tissues,and evaluate the correlation of these expressions with the development of gastric cancer.Methods:The expressions of caveolin-1,E-cadherin and β-catenin were detected by biotin-streptavidinperoxidase (SP) immunohistochemistry on 58 gastric cancer tissues,40 precancerous gastric tissues and 42 chronic non-atrophic gastritis tissues.The correlation between the expressions of caveolin-1,E-cadherin and β-catenin,and the clinicopathologic parameters of gastric cancer was analyzed retrospectively.Results:The positive rates of caveolin-1 and E-cadherin expressions in gastric carcinoma were significantly lower than precancerous gastric and chronic non-atrophic gastritis tissues (P<0.01).An abnormal rate of β-catenin expression in gastric carcinoma was higher than precancerous gastric and chronic non-atrophic gastritis tissues (P<0.01).Moreover,low expressions of caveolin-1,E-cadherin and β-catenin correlated with tumor size,depth of invasion,lymph node metastasis and TNM stage (P<0.05).The positive rates of caveolin-1 and E-cadherin expressions decreased (P<0.01),while an abnormal rate of β-catenin expression increased inversely,with the degree of atypical hyperplasia (P<0.01).Caveolin-1 expression correlated positively with E-cadherin (r=0.41,P<0.05).Caveolin-1 (r=-0.36,P<0.05) and E-cadherin (r=-0.45,P<0.05) expressions negatively correlated with abnormal β-catenin expression.Conclusion:These results suggested that dysregulated expressions of caveolin-1,E-cadherin and β-catenin correlated with the development of gastric cancer and its biological behavior.

  19. Role of caveolin-1 expression in the pathogenesis of pulmonary edema in ventilator-induced lung injury

    OpenAIRE

    Maniatis, Nikolaos A; Kardara, Matina; Hecimovich, Dan; Letsiou, Eleftheria; Castellon, Maricela; Roussos, Charalambos; Shinin, Vasily; Votta-Vellis, E. Gina; Schwartz, David E.; Minshall, Richard D.

    2012-01-01

    Caveolin-1 is a key regulator of pulmonary endothelial barrier function. Here, we tested the hypothesis that caveolin-1 expression is required for ventilator-induced lung injury (VILI). Caveolin-1 gene-disrupted (Cav-1-/-) and age-, sex-, and strain-matched wild-type (WT) control mice were ventilated using two protocols: volume-controlled with protective (8 mL/kg) versus injurious (21 mL/Kg) tidal volume for up to 6 hours; and pressure-controlled with protective (airway pressure = 12 cm H2O) ...

  20. Interphase adhesion geometry is transmitted to an internal regulator for spindle orientation via caveolin-1

    OpenAIRE

    Matsumura, Shigeru; Kojidani, Tomoko; Kamioka, Yuji; Uchida, Seiichi; Haraguchi, Tokuko; Kimura, Akatsuki; Toyoshima, Fumiko

    2016-01-01

    Despite theoretical and physical studies implying that cell-extracellular matrix adhesion geometry governs the orientation of the cell division axis, the molecular mechanisms that translate interphase adhesion geometry to the mitotic spindle orientation remain elusive. Here, we show that the cellular edge retraction during mitotic cell rounding correlates with the spindle axis. At the onset of mitotic cell rounding, caveolin-1 is targeted to the retracting cortical region at the proximal end ...

  1. Decreased caveolin-1 levels contribute to fibrosis and deposition of extracellular IGFBP-5

    OpenAIRE

    Yamaguchi, Yukie; Yasuoka, Hidekata; Stolz, Donna B.; Feghali-Bostwick, Carol A.

    2010-01-01

    Abstract Our previous studies have demonstrated increased expression of insulin-like growth factor binding protein-5 (IGFBP-5) in fibrotic tissues and IGFBP-5 induction of extracellular matrix (ECM) components. The mechanism resulting in increased IGFBP-5 in the extracellular milieu of fibrotic fibroblasts is unknown. Since Caveolin-1 (Cav-1) has been implicated to play a role in membrane trafficking and signal transduction in tissue fibrosis, we examined the effect of Cav-1 on IGFBP-5 intern...

  2. Rosiglitazone ameliorates diffuse axonal injury by reducing loss of tau and up-regulating caveolin-1 expression

    Institute of Scientific and Technical Information of China (English)

    Yong-lin Zhao; Jin-ning Song; Xu-dong Ma; Bin-fei Zhang; Dan-dong Li; Hong-gang Pang

    2016-01-01

    Rosiglitazone up-regulates caveolin-1 levels and has neuroprotective effects in both chronic and acute brain injury. Therefore, we postu-lated that rosiglitazone may ameliorate diffuse axonal injuryvia its ability to up-regulate caveolin-1, inhibit expression of amyloid-beta precursor protein, and reduce the loss and abnormal phosphorylation of tau. In the present study, intraperitoneal injection of rosiglitazone signiifcantly reduced the levels ofamyloid-beta precursor protein and hyperphosphorylated tau (phosphorylated at Ser404 (p-tau (S404)), and it increased the expression of total tau and caveolin-1 in the rat cortex. Our results show that rosiglitazone inhibits the expression of amyloid-beta precursor protein and lowers p-tau (S404) levels, and it reduces the loss of total tau, possibly by up-regulating caveolin-1. These actions of rosiglitazone may underlie its neuroprotective effects in the treatment of diffuse axonal injury.

  3. Rosiglitazone ameliorates diffuse axonal injury by reducing loss of tau and up-regulating caveolin-1 expression

    Directory of Open Access Journals (Sweden)

    Yong-lin Zhao

    2016-01-01

    Full Text Available Rosiglitazone up-regulates caveolin-1 levels and has neuroprotective effects in both chronic and acute brain injury. Therefore, we postulated that rosiglitazone may ameliorate diffuse axonal injury via its ability to up-regulate caveolin-1, inhibit expression of amyloid-beta precursor protein, and reduce the loss and abnormal phosphorylation of tau. In the present study, intraperitoneal injection of rosiglitazone significantly reduced the levels of amyloid-beta precursor protein and hyperphosphorylated tau (phosphorylated at Ser 404 (p-tau (S 404 , and it increased the expression of total tau and caveolin-1 in the rat cortex. Our results show that rosiglitazone inhibits the expression of amyloid-beta precursor protein and lowers p-tau (S 404 levels, and it reduces the loss of total tau, possibly by up-regulating caveolin-1. These actions of rosiglitazone may underlie its neuroprotective effects in the treatment of diffuse axonal injury.

  4. Rosiglitazone ameliorates diffuse axonal injury by reducing loss of tau and up-regulating caveolin-1 expression

    Science.gov (United States)

    Zhao, Yong-lin; Song, Jin-ning; Ma, Xu-dong; Zhang, Bin-fei; Li, Dan-dong; Pang, Hong-gang

    2016-01-01

    Rosiglitazone up-regulates caveolin-1 levels and has neuroprotective effects in both chronic and acute brain injury. Therefore, we postulated that rosiglitazone may ameliorate diffuse axonal injury via its ability to up-regulate caveolin-1, inhibit expression of amyloid-beta precursor protein, and reduce the loss and abnormal phosphorylation of tau. In the present study, intraperitoneal injection of rosiglitazone significantly reduced the levels of amyloid-beta precursor protein and hyperphosphorylated tau (phosphorylated at Ser404(p-tau (S404)), and it increased the expression of total tau and caveolin-1 in the rat cortex. Our results show that rosiglitazone inhibits the expression of amyloid-beta precursor protein and lowers p-tau (S404) levels, and it reduces the loss of total tau, possibly by up-regulating caveolin-1. These actions of rosiglitazone may underlie its neuroprotective effects in the treatment of diffuse axonal injury.

  5. Binding of nuclear caveolin-1 to promoter elements of growth-associated genes in ovarian carcinoma cells

    International Nuclear Information System (INIS)

    Caveolin-1 (cav-1), a member of a protein family associated mainly with cell membrane microdomains in many cell types, acts as a tumor suppressor in ovarian carcinoma cells. Biochemical analyses demonstrated that cav-1 was also localized in the nuclei of ovarian carcinoma cells, endogenously (SKOV3) or ectopically (IGtC3) expressing cav-1. By confocal analyses, the same cell lines as well as IGROV1 and SKOV3 cells transiently transfected with green fluorescent protein-cav-1 fusion protein showed nuclear punctate speckled pattern. Subnuclear distribution analysis revealed cav-1 mainly associated with the nuclear matrix, but also slightly with chromatin. Cav-1 was found in nuclear high-molecular weight complexes and by confocal analysis was found to co-localized with the inner nuclear membrane protein emerin. Cyclin D1 and folate receptor promoters were modulated by cav-1 in SKOV3 cells as demonstrated by transient transfection with or silencing of cav-1. Chromatin immunoprecipitation and supershift assays indicated that nuclear cav-1 can bind in vitro and in vivo to promoter sequences of both cyclin D1 and folate receptor genes. These data suggest that in ovarian carcinoma cells cav-1, localized in transcriptionally inactive chromatin, exerts a functional activity mediated, at least in part, by directly binding to sequences of genes involved in proliferation

  6. Polychlorinated biphenyl-induced VCAM-1 expression is attenuated in aortic endothelial cells isolated from caveolin-1 deficient mice

    International Nuclear Information System (INIS)

    Exposure to environmental contaminants, such as polychlorinated biphenyls (PCBs), is a risk factor for the development of cardiovascular diseases such as atherosclerosis. Vascular cell adhesion molecule-1 (VCAM-1) is a critical mediator for adhesion and uptake of monocytes across the endothelium in the early stages of atherosclerosis development. The upregulation of VCAM-1 by PCBs may be dependent on functional membrane domains called caveolae. Caveolae are particularly abundant in endothelial cell membranes and involved in trafficking and signal transduction. The objective of this study was to investigate the role of caveolae in PCB-induced endothelial cell dysfunction. Primary mouse aortic endothelial cells (MAECs) isolated from caveolin-1-deficient mice and background C57BL/6 mice were treated with coplanar PCBs, such as PCB77 and PCB126. In addition, siRNA gene silencing technique was used to knockdown caveolin-1 in porcine vascular endothelial cells. In MAECs with functional caveolae, VCAM-1 protein levels were increased after exposure to both coplanar PCBs, whereas expression levels of VCAM-1 were not significantly altered in cells deficient of caveolin-1. Furthermore, PCB-induced monocyte adhesion was attenuated in caveolin-1-deficient MAECs. Similarly, siRNA silencing of caveolin-1 in porcine endothelial cells confirmed the caveolin-1-dependent VCAM-1 expression. Treatment of cells with PCB77 and PCB126 resulted in phosphorylation of extracellular signal-regulated kinase-1/2 (ERK1/2), and pharmacological inhibition of ERK1/2 diminished the observed PCB-induced increase in monocyte adhesion. These findings suggest that coplanar PCBs induce adhesion molecule expression, such as VCAM-1, in endothelial cells, and that this response is regulated by caveolin-1 and functional caveolae. Our data demonstrate a critical role of functional caveolae in the activation and dysfunction of endothelial cells by coplanar PCBs.

  7. Enhanced subbarrier fusion for proton halo nuclei

    Science.gov (United States)

    Kumar, Raj; Lay, J. A.; Vitturi, A.

    2014-02-01

    In this Brief Report we use a simple model to describe the dynamical effects of break-up processes in the subbarrier fusion involving weakly bound nuclei. We model two similar cases involving either a neutron or a proton halo nucleus, both schematically coupled to the break-up channels. We find that the decrease of the Coulomb barrier in the proton break-up channel leads, ceteris paribus, to a larger enhancement of the subbarrier fusion probabilities with respect to the neutron halo case.

  8. Enhanced subbarrier fusion for proton halo nuclei

    OpenAIRE

    Kumar, Raj; J.A. Lay; Vitturi, A.

    2014-01-01

    In this short note we use a simple model to describe the dynamical effects of break-up processes in the subbarrier fusion involving weakly bound nuclei. We model two similar cases involving either a neutron or a proton halo nucleus, both schematically coupled to the break-up channels. We find that the decrease of the coulomb barrier in the proton break-up channel leads, ceteris paribus, to a larger enhancement of the subbarier fusion probabilities with respect to the neutron-halo case.

  9. Smooth muscle NOS, colocalized with caveolin-1, modulates contraction in mouse small intestine

    OpenAIRE

    El-Yazbi, Ahmed F.; Cho, Woo Jung; Cena, Jonathan; Schulz, Richard; Daniel, Edwin E

    2008-01-01

    Neuronal nitric oxide synthase (nNOS) in myenteric neurons is activated during peristalsis to produce nitric oxide which relaxes intestinal smooth muscle. A putative nNOS is also found in the membrane of intestinal smooth muscle cells in mouse and dog. In this study we studied the possible functions of this nNOS expressed in mouse small intestinal smooth muscle colocalized with caveolin-1(Cav-1). Cav-1 knockout mice lacked nNOS in smooth muscle and provided control tissues. 60 mM KCl was used...

  10. Enhanced chemical weapon warning via sensor fusion

    Science.gov (United States)

    Flaherty, Michael; Pritchett, Daniel; Cothren, Brian; Schwaiger, James

    2011-05-01

    Torch Technologies Inc., is actively involved in chemical sensor networking and data fusion via multi-year efforts with Dugway Proving Ground (DPG) and the Defense Threat Reduction Agency (DTRA). The objective of these efforts is to develop innovative concepts and advanced algorithms that enhance our national Chemical Warfare (CW) test and warning capabilities via the fusion of traditional and non-traditional CW sensor data. Under Phase I, II, and III Small Business Innovative Research (SBIR) contracts with DPG, Torch developed the Advanced Chemical Release Evaluation System (ACRES) software to support non real-time CW sensor data fusion. Under Phase I and II SBIRs with DTRA in conjunction with the Edgewood Chemical Biological Center (ECBC), Torch is using the DPG ACRES CW sensor data fuser as a framework from which to develop the Cloud state Estimation in a Networked Sensor Environment (CENSE) data fusion system. Torch is currently developing CENSE to implement and test innovative real-time sensor network based data fusion concepts using CW and non-CW ancillary sensor data to improve CW warning and detection in tactical scenarios.

  11. Microparticle-Induced Activation of the Vascular Endothelium Requires Caveolin-1/Caveolae.

    Directory of Open Access Journals (Sweden)

    Allison M Andrews

    Full Text Available Microparticles (MPs are small membrane fragments shed from normal as well as activated, apoptotic or injured cells. Emerging evidence implicates MPs as a causal and/or contributing factor in altering normal vascular cell phenotype through initiation of proinflammatory signal transduction events and paracrine delivery of proteins, mRNA and miRNA. However, little is known regarding the mechanism by which MPs influence these events. Caveolae are important membrane microdomains that function as centers of signal transduction and endocytosis. Here, we tested the concept that the MP-induced pro-inflammatory phenotype shift in endothelial cells (ECs depends on caveolae. Consistent with previous reports, MP challenge activated ECs as evidenced by upregulation of intracellular adhesion molecule-1 (ICAM-1 expression. ICAM-1 upregulation was mediated by activation of NF-κB, Poly [ADP-ribose] polymerase 1 (PARP-1 and the epidermal growth factor receptor (EGFR. This response was absent in ECs lacking caveolin-1/caveolae. To test whether caveolae-mediated endocytosis, a dynamin-2 dependent process, is a feature of the proinflammatory response, EC's were pretreated with the dynamin-2 inhibitor dynasore. Similar to observations in cells lacking caveolin-1, inhibition of endocytosis significantly attenuated MPs effects including, EGFR phosphorylation, activation of NF-κB and upregulation of ICAM-1 expression. Thus, our results indicate that caveolae play a role in mediating the pro-inflammatory signaling pathways which lead to EC activation in response to MPs.

  12. Deletion of Caveolin-1 Protects against Oxidative Lung Injury via Up-Regulation of Heme Oxygenase-1

    OpenAIRE

    Jin, Yang; Kim, Hong Pyo; Chi, Minli; Ifedigbo, Emeka; Stefan W. Ryter; Choi, Augustine M. K.

    2008-01-01

    Acute lung injury (ALI) is a major cause of morbidity and mortality in critically ill patients. Hyperoxia causes lung injury in animals and humans, and is an established model of ALI. Caveolin-1, a major constituent of caveolae, regulates numerous biological processes, including cell death and proliferation. Here we demonstrate that caveolin-1–null mice (cav-1−/−) were resistant to hyperoxia-induced death and lung injury. Cav-1−/− mice sustained reduced lung injury after hyperoxia as determin...

  13. Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO

    OpenAIRE

    Marblestone, Jeffrey G; Edavettal, Suzanne C.; Lim, Yiting; Lim, Peter; Zuo, Xun; Butt, Tauseef R.

    2006-01-01

    Despite the availability of numerous gene fusion systems, recombinant protein expression in Escherichia coli remains difficult. Establishing the best fusion partner for difficult-to-express proteins remains empirical. To determine which fusion tags are best suited for difficult-to-express proteins, a comparative analysis of the newly described SUMO fusion system with a variety of commonly used fusion systems was completed. For this study, three model proteins, enhanced green florescent protei...

  14. Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice

    Science.gov (United States)

    Sala-Vila, Aleix; Navarro-Lérida, Inmaculada; Sánchez-Alvarez, Miguel; Bosch, Marta; Calvo, Carlos; López, Juan Antonio; Calvo, Enrique; Ferguson, Charles; Giacomello, Marta; Serafini, Annalisa; Scorrano, Luca; Enriquez, José Antonio; Balsinde, Jesús; Parton, Robert G.; Vázquez, Jesús; Pol, Albert; Del Pozo, Miguel A.

    2016-01-01

    The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed. PMID:27272971

  15. Effect of FXR on Lipid Metabolism and Caveolin-1 Expression in Hepatocytes%FXR对肝细胞脂质代谢以及Caveolin-1表达的影响

    Institute of Scientific and Technical Information of China (English)

    丁隆; 杨宇; 曲义坤; 夏伟滨; 刘伟新; 张英海; 杨婷; 王凯峰

    2013-01-01

    [目的]探讨法尼醇X受体(FXR)对肝细胞脂质代谢以及小窝蛋白(Caveolin-1)表达的相关性.[方法]培养肝细胞,分别以10 μmol/L的FXR激动剂 GW4064和100 μmol/L的FXR拮抗剂Guggulsterones干预细胞;RT-PCR和Western blot检测小异二聚体伴侣(SHP)、胆固醇7α-羟化酶(CYP7A1)、胆盐输出泵(BSEP)的表达;试剂盒检测肝细胞中甘油三酯以及胆固醇的水平;Western blot检测Caveolin-1的表达.[结果]与对照组相比FXR激动剂组SHP、BSEP、Caveolin-1表达显著升高(P<0.05),CYP7A1表达显著降低(P<0.05),FXR拮抗剂组SHP、BSEP、Caveolin-1表达显著降低(P<0.05),CYP7A1显著升高(P<0.05).FXR激动剂组甘油三酯水平显著降低,胆固醇含量显著升高,而FXR拮抗剂组甘油三酯显著升高,胆固醇含量显著降低,差异具有统计学意义(P<0.05).[结论]FXR在调控肝细胞代谢中发挥重要作用,并且可能通过对胆固醇代谢的调控影响Caveolin-1的表达.%[Objective] To explore the effect of farnesoid X receptor(FXR) on lipid metabolism and caveolin-1 expression in hepatocytes. [Methods] Hepatocytes were cultured by the treatment with 10μmol/L FXR agonist GW4064 or 100μmol/L FXR antagonist guggulsterones, respectively. The expressions of SHP, CYP7A1 and BSEP were detected by RT-PCR and Western blot. The kit was used to determine the levels of triglyceride and cholesterol in hepatocytes. Western blot was used to detect the expression of caveolin-1. [Results]Compared with control group, the expressions of SHP, BSEP and caveolin-1 markedly increased( P <0.05) and the expression of CYP7A1 markedly decreased in FXR agonist group( P<0. 05). In FXR antagonist group, the expressions of SHP, BSEP and caveolin-1 in decreased markedly( P<0.05), while the expression of CYP7A1 increased marked ly ( P <0. 05). Triglyceride level markedly decreased and cholesterol level markedly increased in FXR agonist group, while riglyceride level markedly increased and cholesterol

  16. Altered monocyte and fibrocyte phenotype and function in scleroderma interstitial lung disease: reversal by caveolin-1 scaffolding domain peptide

    Directory of Open Access Journals (Sweden)

    Tourkina Elena

    2011-07-01

    Full Text Available Abstract Interstitial lung disease (ILD is a major cause of morbidity and mortality in scleroderma (systemic sclerosis, or SSc. Fibrocytes are a monocyte-derived cell population implicated in the pathogenesis of fibrosing disorders. Given the recently recognized importance of caveolin-1 in regulating function and signaling in SSc monocytes, in the present study we examined the role of caveolin-1 in the migration and/or trafficking and phenotype of monocytes and fibrocytes in fibrotic lung disease in human patients and an animal model. These studies fill a gap in our understanding of how monocytes and fibrocytes contribute to SSc-ILD pathology. We found that C-X-C chemokine receptor type 4-positive (CXCR4+/collagen I-positive (ColI+, CD34+/ColI+ and CD45+/ColI+ cells are present in SSc-ILD lungs, but not in control lungs, with CXCR4+ cells being most prevalent. Expression of CXCR4 and its ligand, stromal cell-derived factor 1 (CXCL12, are also highly upregulated in SSc-ILD lung tissue. SSc monocytes, which lack caveolin-1 and therefore overexpress CXCR4, exhibit almost sevenfold increased migration toward CXCL12 compared to control monocytes. Restoration of caveolin-1 function by administering the caveolin scaffolding domain (CSD peptide reverses this hypermigration. Similarly, transforming growth factor β-treated normal monocytes lose caveolin-1, overexpress CXCR4 and exhibit 15-fold increased monocyte migration that is CSD peptide-sensitive. SSc monocytes exhibit a different phenotype than normal monocytes, expressing high levels of ColI, CD14 and CD34. Because ColI+/CD14+ cells are prevalent in SSc blood, we looked for such cells in lung tissue and confirmed their presence in SSc-ILD lungs but not in normal lungs. Finally, in the bleomycin model of lung fibrosis, we show that CSD peptide diminishes fibrocyte accumulation in the lungs. Our results suggest that low caveolin-1 in SSc monocytes contributes to ILD via effects on cell migration and

  17. Pilus phase variation switches gonococcal adherence to invasion by caveolin-1-dependent host cell signaling.

    Science.gov (United States)

    Faulstich, Michaela; Böttcher, Jan-Peter; Meyer, Thomas F; Fraunholz, Martin; Rudel, Thomas

    2013-01-01

    Many pathogenic bacteria cause local infections but occasionally invade into the blood stream, often with fatal outcome. Very little is known about the mechanism underlying the switch from local to invasive infection. In the case of Neisseria gonorrhoeae, phase variable type 4 pili (T4P) stabilize local infection by mediating microcolony formation and inducing anti-invasive signals. Outer membrane porin PorB(IA), in contrast, is associated with disseminated infection and facilitates the efficient invasion of gonococci into host cells. Here we demonstrate that loss of pili by natural pilus phase variation is a prerequisite for the transition from local to invasive infection. Unexpectedly, both T4P-mediated inhibition of invasion and PorB(IA)-triggered invasion utilize membrane rafts and signaling pathways that depend on caveolin-1-Y14 phosphorylation (Cav1-pY14). We identified p85 regulatory subunit of PI3 kinase (PI3K) and phospholipase Cγ1 as new, exclusive and essential interaction partners for Cav1-pY14 in the course of PorBIA-induced invasion. Active PI3K induces the uptake of gonococci via a new invasion pathway involving protein kinase D1. Our data describe a novel route of bacterial entry into epithelial cells and offer the first mechanistic insight into the switch from local to invasive gonococcal infection. PMID:23717204

  18. Pilus phase variation switches gonococcal adherence to invasion by caveolin-1-dependent host cell signaling.

    Directory of Open Access Journals (Sweden)

    Michaela Faulstich

    Full Text Available Many pathogenic bacteria cause local infections but occasionally invade into the blood stream, often with fatal outcome. Very little is known about the mechanism underlying the switch from local to invasive infection. In the case of Neisseria gonorrhoeae, phase variable type 4 pili (T4P stabilize local infection by mediating microcolony formation and inducing anti-invasive signals. Outer membrane porin PorB(IA, in contrast, is associated with disseminated infection and facilitates the efficient invasion of gonococci into host cells. Here we demonstrate that loss of pili by natural pilus phase variation is a prerequisite for the transition from local to invasive infection. Unexpectedly, both T4P-mediated inhibition of invasion and PorB(IA-triggered invasion utilize membrane rafts and signaling pathways that depend on caveolin-1-Y14 phosphorylation (Cav1-pY14. We identified p85 regulatory subunit of PI3 kinase (PI3K and phospholipase Cγ1 as new, exclusive and essential interaction partners for Cav1-pY14 in the course of PorBIA-induced invasion. Active PI3K induces the uptake of gonococci via a new invasion pathway involving protein kinase D1. Our data describe a novel route of bacterial entry into epithelial cells and offer the first mechanistic insight into the switch from local to invasive gonococcal infection.

  19. Molecular characterization and expression analysis of caveolin-1 in pig tissues

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Members of the caveolin family played important roles during fundamental cellular processes,such as regulation of cell morphology,migration,and gene expression in muscle cells.In this study,caveolin-1 (Cav-1),one of the caveolins,was identified from longissimus dorsi muscle of Large Yorkshire pig and Chinese indigenous Lantang pig based on the results of mRNA differential display analysis.The deduced amino acids sequence of the porcine Cav-1 contained a caveolin domain,and was very conservative among different species.The Cav-1 mRNA was widely expressed in the eight tissues in this study,including heart,liver,kidney,encephalon,spleen,lung,longissimus dorsi muscle,and back fat, and the highest expression quantity was found in back fat of the two pig breeds.The expression quantity of porcine Cav-1 in back fat and longissimus dorsi muscle of Lantang pig was significantly higher than that of Large Yorkshire(P<0.01,and P<0.05,respectively).These results suggested that the Cav-1 might be a candidate gene for carcass traits,and might provide valuable information for understanding the mechanism of caveolae signaling in fat deposition by using the animal model of pig.

  20. New glimpses of caveolin-1 functions in embryonic development and human diseases

    Institute of Scientific and Technical Information of China (English)

    Saijun MO; Shengli YANG; Zongbin CUI

    2011-01-01

    Caveolin-1 (Cav-1) isoforms,including Cav-1α and Cav-1β,were identified as integral membrane proteins and the major components of caveolae.Cav-1 proteins are highly conserved during evolution from Caenorhabditis elegans to human and are capable of interacting with many signaling molecules through their caveolin scaffolding domains to regulate the activities of multiple signaling pathways.Thus,Cav-1 plays crucial roles in the regulation of cellular proliferation,differentiation and apoptosis in a cell-specific and contextual manner.In addition,Cav-1 is essential for embryonic development of vertebrates owing to its regulation of BMP,Wnt,TGF-β and other key signaling molecules.Moreover,Cav-1 is mainly expressed in terminally differentiated cells and its abnormal expression is often associated with human diseases,such as tumor progression,cardiovascular diseases,fibrosis,lung regeneration,and diseases related to virus.In this review,we will further discuss the potential of Cav-1 as a target for disease therapy and multiple drug resistance.

  1. Serum Caveolin-1 as a Novel Biomarker in Idiopathic Pulmonary Artery Hypertension

    Directory of Open Access Journals (Sweden)

    Kuo-Yang Wang

    2015-01-01

    Full Text Available Pulmonary arterial hypertension (PAH is a rare disease but with significant morbidity and high mortality. There is no specific way to diagnose PAH. Thus, an easy used with good sensitivity and specificity biomarker of PAH is highly desirable to aid in the screening, diagnosis, and follow-up. Caveolin-1 (Cav1 is the structural protein of caveolae and is highly expressed in type I pneumocytes. Lungs tissues from idiopathic PAH (IPAH patients showed decreased expression of Cav1 in vascular endothelial cells. Therefore, we developed a direct sandwich immunoassay for the determination of Cav1 in IAPH patient’s serum. The result disclosed serum Cav1 level was significantly lower in IPAH than control groups. Using serum Cav1, 17.17 pg/mL as a cutoff value, the sensitivity was 0.59 and the specificity was 1.0. There were two major findings in our results. First, serum Cav1 might be a novel biomarker in the diagnosis of IPAH with fare sensitivity and good specificity. Second, Cav1 might be used to make differential diagnosis between COPD-PH and IPAH group.

  2. Expression of Caveolin-1 in tongue squamous cell carcinoma by quantum dots

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    J. Xue

    2010-04-01

    Full Text Available Quantum dots (QDs are a new class of fluorescent probes to detect biomarker expression. The role of caveolin-1 (Cav-1 in tongue squamous cell carcinoma (TSCC is still unknown. This study aimed to investigate the expression profile of Cav-1 in carcinogenesis and development of TSCC by QDs immunofluorescence histochemistry (QDs-IHC and discuss the relationship between the Cav-1 expression and the clinicopathological outcomes. QDs-IHC was used to detect Cav-1 expression in tissue microarrays including normal tongue mucosa (NTM; n=10, hyperplastic tongue mucosa (HTM; n=10, tongue pre-cancer lesions (TPL; n=15 and primary tongue squamous cell carcinoma (PTSCC; n=61. Correlations between the Cav-1 expression and clinicopathologic variables were evaluated statistically. Cells positive for Cav-1 were clearly detected and bright images were obtained in a fine, granular pattern at the cell membrane and cytoplasm using QDs-IHC. The rate of Cav-1 immunoreactivity increased progressively from NTM (0%, HTM (0%, TPL (36% to PTSCC (74%. When compared with each other, there was statistical significance among PTSCC, TPL and NTM as well as among PTSCC, TPL and HTM. Moreover, Cav-1 expression level in PTSCC was correlated positively with clinical stage and histologic grade. QDs-IHC could accurately detect protein location in tongue mucosa. An increased expression of Cav-1 in the stepwise carcinogenesis from NTM, HTM, TPL to PTSCC suggested that Cav-1 might be an oncogene in the development of tongue squamous cell carcinoma.

  3. Molecular characterization and expression analysis of caveolin-1 in pig tissues

    Institute of Scientific and Technical Information of China (English)

    WANG Chong; MEI YingJie; LI Li; MO DeLin; LI JiaQi; ZHANG Hao; TIAN XingGuo; CHEN YaoSheng

    2008-01-01

    Members of the caveolin family played important roles during fundamental cellular processes, such as regulation of cell morphology, migration, and gene expression in muscle cells. In this study, caveolin-1 (Cav-1), one of the caveolins, was identified from Iongissimus dorsi muscle of Large Yorkshire pig and Chinese indigenous Lantang pig based on the results of mRNA differential display analysis. The de-duced amino acids sequence of the porcine Cav-1 contained a caveolin domain, and was very conser-vative among different species. The Cav-1 mRNA was widely expressed in the eight tissues in this study, including heart, liver, kidney, encephalon, spleen, lung, Iongissimus dorsi muscle, and back fat, and the highest expression quantity was found in back fat of the two pig breeds. The expression quan-tity of porcine Car-1 in back fat and Iongissimus dorsi muscle of Lantang pig was significantly higher than that of Large Yorkshire (P<0.01, and P<0.05, respectively). These results suggested that the Cav-1 might be a candidate gene for carcass traits, and might provide valuable information for understanding the mechanism of caveolae signaling in fat deposition by using the animal model of pig.

  4. Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours.

    Science.gov (United States)

    Ebisawa, M; Iwano, H; Nishikawa, M; Tochigi, Y; Komatsu, T; Endou, Y; Hirayama, K; Taniyama, H; Kadosawa, T; Yokota, H

    2015-11-01

    Canine mammary tumours (CMTs) are the most common neoplasms affecting female dogs. There is an urgent need for molecular biomarkers that can detect early stages of the disease in order to improve accuracy of CMT diagnosis. The aim of this study was to examine whether caveolin-1 (Cav-1) and matrix metalloproteinase 14 (MMP14) are associated with CMT histological malignancy and invasion. Sixty-five benign and malignant CMT samples and six normal canine mammary glands were analysed using quantitative reverse transcription-polymerase chain reaction. Cav-1 and MMP14 genes were highly expressed in CMT tissues compared to normal tissues. Cav-1 especially was overexpressed in malignant and invasive CMT tissues. When a CMT cell line was cultured on fluorescent gelatin-coated coverslips, localisation of Cav-1 was observed at invadopodia-mediated degradation sites of the gelatin matrix. These findings suggest that Cav-1 may be involved in CMT invasion and that the markers may be useful for estimating CMT malignancy. PMID:26364240

  5. Caveolin-1 Expression Level in Cancer Associated Fibroblasts Predicts Outcome in Gastric Cancer

    Science.gov (United States)

    Gao, Jun; Fan, Lifang; Li, Zonghuan; Yang, Guifang; Chen, Honglei

    2013-01-01

    Aims Altered expression of epithelial or stromal caveolin-1 (Cav-1) is observed in various types of human cancers. However, the clinical significance of Cav-1 expression in gastric cancer (GC) remains largely unknown. The present study aims to explore the clinicopathological significance and prognostic value of both tumor cells and cancer associated fibroblasts (CAFs) Cav-1 in GC. Methods and Results Quantum dots immunofluorescence histochemistry was performed to examine the expression of Cav-1 in 20 cases of gastritis without intestinal metaplasia (IM), 20 cases of gastritis with IM and 286 cases of GC. Positive rates of epithelial Cav-1 in gastritis without IM, gastritis with IM and GC showed a decreasing trend (P = 0.012). Low expression of Cav-1 in CAFs but not in tumor cells was an independent predictor of poor prognosis in GC patients (P = 0.034 and 0.005 respectively in disease free survival and overall survival). Cav-1 level in tumor cells and CAFs showed no significant correlation with classic clinicopathological features. Conclusions Loss of epithelial Cav-1 may promote malignant progression and low CAFs Cav-1 level herald worse outcome of GC patient, suggesting CAFs Cav-1 may be a candidate therapeutic target and a useful prognostic marker of GC. PMID:23527097

  6. ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1.

    Science.gov (United States)

    Yamaguchi, Tomoya; Lu, Can; Ida, Lisa; Yanagisawa, Kiyoshi; Usukura, Jiro; Cheng, Jinglei; Hotta, Naoe; Shimada, Yukako; Isomura, Hisanori; Suzuki, Motoshi; Fujimoto, Toyoshi; Takahashi, Takashi

    2016-01-01

    The receptor tyrosine kinase-like orphan receptor 1 (ROR1) sustains prosurvival signalling directly downstream of the lineage-survival oncogene NKX2-1/TTF-1 in lung adenocarcinoma. Here we report an unanticipated function of this receptor tyrosine kinase (RTK) as a scaffold of cavin-1 and caveolin-1 (CAV1), two essential structural components of caveolae. This kinase-independent function of ROR1 facilitates the interactions of cavin-1 and CAV1 at the plasma membrane, thereby preventing the lysosomal degradation of CAV1. Caveolae structures and prosurvival signalling towards AKT through multiple RTKs are consequently sustained. These findings provide mechanistic insight into how ROR1 inhibition can overcome EGFR-tyrosine kinase inhibitor (TKI) resistance due to bypass signalling via diverse RTKs such as MET and IGF-IR, which is currently a major clinical obstacle. Considering its onco-embryonic expression, inhibition of the scaffold function of ROR1 in patients with lung adenocarcinoma is an attractive approach for improved treatment of this devastating cancer. PMID:26725982

  7. Regulation of caveolin-1 expression, nitric oxide production and tissue injury by tumor necrosis factor-α following ozone inhalation

    International Nuclear Information System (INIS)

    Alveolar macrophages (AM) and inflammatory mediators including nitric oxide and peroxynitrite contribute to ozone-induced lung injury. The generation of these mediators is regulated, in part, by the transcription factor NF-κB. We previously demonstrated a critical role for NF-κB p50 in ozone-induced injury. In the present studies mechanisms regulating NF-κB activation in the lung after ozone inhalation were analyzed. Treatment of wild type (WT) mice with ozone (0.8 ppm, 3 h) resulted in a rapid increase in NF-κB binding activity in AM, which persisted for at least 12 h. This was not evident in mice lacking TNFα which are protected from ozone-induced injury; there was also no evidence of nitric oxide or peroxynitrite production in lungs from these animals. These data demonstrate that TNFα plays a role in NF-κB activation and toxicity. TNFα signaling involves PI-3-kinase (PI3K)/protein kinase B (PKB), and p44/42 MAP kinase (MAPK) which are important in NF-κB activation. Ozone Inhalation resulted in rapid and transient increases in p44/42 MAPK and PI3K/PKB in AM from WT mice, which was evident immediately after exposure. Caveolin-1, a transmembrane protein that negatively regulates PI3K/PKB and p44/42 MAPK signaling, was downregulated in AM from WT mice after ozone exposure. In contrast, ozone had no effect on caveolin-1, PI3K/PKB or p44/42 MAPK expression in AM from TNFα knockout mice. These data, together with our findings that TNFα suppressed caveolin-1 expression in cultured AM, suggest that TNFα and downstream signaling mediate activation of NF-κB and the regulation of inflammatory genes important in ozone toxicity, and that this process is linked to caveolin-1

  8. Caveolin-1 plays a critical role in host immunity against Klebsiella pneumoniae by regulating STAT5 and Akt activity

    OpenAIRE

    Guo, Qiang; Shen, Nan; Yuan, Kefei; Li, Jiaxin; Wu, Hong; Zeng, Yong; Fox, John; Bansal, Arvind K.; Singh, Brij B; Gao, Hongwei; Wu, Min

    2012-01-01

    Caveolin-1 (Cav1) is a structural protein of caveolae. Although Cav1 is associated with certain bacterial infections, it is unknown whether Cav1 is involved in host immunity against Klebsiella pneumoniae, the third most commonly isolated microorganism from bacterial sepsis patients. Here, we showed that cav1 knockout mice succumbed to K. pneumoniae infection with markedly decreased survival rates, increased bacterial burdens, intensified tissue injury, hyperactive proinflammatory cytokines, a...

  9. Auto-stimulatory action of secreted caveolin-1 on the proliferation of Ewing’s sarcoma cells

    OpenAIRE

    Sengupta, Aniruddha; Mateo-Lozano, Silvia; Tirado, Oscar M.; Notario, Vicente

    2011-01-01

    Caveolin-1 (CAV1) is highly expressed in Ewing’s sarcoma (EWS). We previously showed that increased cellular CAV1 is associated with the regulation of the tumorigenicity, drug resistance and metastatic ability of EWS cells. Because several studies reported that melanoma and prostate cancer cells, which express relatively high CAV1 levels, secrete CAV1, and that secreted CAV1 is associated with tumor progression, our study explored the possibility that EWS cells also secreted CAV1 and that sec...

  10. Neuronal differentiation dictates estrogen-dependent survival and ERK1/2 kinetic by means of caveolin-1.

    Directory of Open Access Journals (Sweden)

    Floriana Volpicelli

    Full Text Available Estrogens promote a plethora of effects in the CNS that profoundly affect both its development and mature functions and are able to influence proliferation, differentiation, survival and neurotransmission. The biological effects of estrogens are cell-context specific and also depend on differentiation and/or proliferation status in a given cell type. Furthermore, estrogens activate ERK1/2 in a variety of cellular types. Here, we investigated whether ERK1/2 activation might be influenced by estrogens stimulation according to the differentiation status and the molecular mechanisms underling this phenomenon. ERK1/2 exert an opposing role on survival and death, as well as on proliferation and differentiation depending on different kinetics of phosphorylation. Hence we report that mesencephalic primary cultures and the immortalized cell line mes-c-myc A1 express estrogen receptor α and activate ERK1/2 upon E2 stimulation. Interestingly, following the arrest of proliferation and the onset of differentiation, we observe a change in the kinetic of ERKs phosphorylation induced by estrogens stimulation. Moreover, caveolin-1, a main constituent of caveolae, endogenously expressed and co-localized with ER-α on plasma membrane, is consistently up-regulated following differentiation and cell growth arrest. In addition, we demonstrate that siRNA-induced caveolin-1 down-regulation or disruption by means of ß-cyclodextrin treatment changes ERK1/2 phosphorylation in response to estrogens stimulation. Finally, caveolin-1 down-regulation abolishes estrogens-dependent survival of neurons. Thus, caveolin-1 appears to be an important player in mediating, at least, some of the non-genomic action of estrogens in neurons, in particular ERK1/2 kinetics of activation and survival.

  11. Caveolin-1 gene silencing promotes the activation of PI3K/AKT dependent on Erα36 and the transformation of MCF10ACE

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    ERα36,a variant of estrogen receptor-α,acts as a dominant-negative factor in both estrogen-dependent and estrogen-independent transactivation signaling pathways,and is a key factor in the promotion,progression and prognosis of breast cancers.Caveolin-1,a 22-to 24-kD integral membrane protein,may function as a tumor suppressor in inhibiting of many growth-promoting signaling pathways.It was shown that downregulation of Caveolin-1 strengthens the interaction of ERα and Caveolin-1.In conclusion,Caveolin-1 gene silencing activated the PI3K/AKT signaling pathway in an ERα36-dependent way.Our finding may provide a promising therapeutic target of breast cancer.

  12. Enhanced Face Recognition using Data Fusion

    Directory of Open Access Journals (Sweden)

    Alaa Eleyan

    2012-12-01

    Full Text Available In this paper we scrutinize the influence of fusion on the face recognition performance. In pattern recognition task, benefiting from different uncorrelated observations and performing fusion at feature and/or decision levels improves the overall performance. In features fusion approach, we fuse (concatenate the feature vectors obtained using different feature extractors for the same image. Classification is then performed using different similarity measures. In decisions fusion approach, the fusion is performed at decisions level, where decisions from different algorithms are fused using majority voting. The proposed method was tested using face images having different facial expressions and conditions obtained from ORL and FRAV2D databases. Simulations results show that the performance of both feature and decision fusion approaches outperforms the single performances of the fused algorithms significantly.

  13. Cellular Prion Protein and Caveolin-1 Interaction in a Neuronal Cell Line Precedes Fyn/Erk 1/2 Signal Transduction

    Directory of Open Access Journals (Sweden)

    Mattia Toni

    2006-01-01

    Full Text Available It has been reported that cellular prion protein (PrPc is enriched in caveolae or caveolae-like domains with caveolin-1 (Cav-1 participating to signal transduction events by Fyn kinase recruitment. By using the Glutathione-S-transferase (GST-fusion proteins assay, we observed that PrPc strongly interacts in vitro with Cav-1. Thus, we ascertained the PrPc caveolar localization in a hypothalamic neuronal cell line (GN11, by confocal microscopy analysis, flotation on density gradient, and coimmunoprecipitation experiments. Following the anti-PrPc antibody-mediated stimulation of live GN11 cells, we observed that PrPc clustered on plasma membrane domains rich in Cav-1 in which Fyn kinase converged to be activated. After these events, a signaling cascade through p42/44 MAP kinase (Erk 1/2 was triggered, suggesting that following translocations from rafts to caveolae or caveolae-like domains PrPc could interact with Cav-1 and induce signal transduction events.

  14. Caveolin-1 protects against hepatic ischemia/reperfusion injury through ameliorating peroxynitrite-mediated cell death.

    Science.gov (United States)

    Gao, Lei; Chen, Xingmiao; Peng, Tao; Yang, Dan; Wang, Qi; Lv, Zhiping; Shen, Jiangang

    2016-06-01

    Nitrative stress is considered as an important pathological process of hepatic ischemia and reperfusion injury but its regulating mechanisms are largely unknown. In this study, we tested the hypothesis that caveolin-1 (Cav-1), a plasma membrane scaffolding protein, could be an important cellular signaling against hepatic I/R injury through inhibiting peroxynitrite (ONOO(-))-induced cellular damage. Male wild-type mice and Cav-1 knockout (Cav-1(-/-)) were subjected to 1h hepatic ischemia following 1, 6 and 12h of reperfusion by clipping and releasing portal vessels respectively. Immortalized human hepatocyte cell line (L02) was subjected to 1h hypoxia and 6h reoxygenation and treated with Cav-1 scaffolding domain peptide. The major discoveries included: (1) the expression of Cav-1 in serum and liver tissues of wild-type mice was time-dependently elevated during hepatic ischemia-reperfusion injury. (2) Cav-1 scaffolding domain peptide treatment inhibited cleaved caspase-3 expression in the hypoxia-reoxygenated L02 cells; (3) Cav-1 knockout (Cav-1(-/-)) mice had significantly higher levels of serum transaminases (ALT&AST) and TNF-α, and higher rates of apoptotic cell death in liver tissues than wild-type mice after subjected to 1h hepatic ischemia and 6hour reperfusion; (4) Cav-1(-/-) mice revealed higher expression levels of iNOS, ONOO(-) and 3-nitrotyrosine (3-NT) in the liver than wild-type mice, and Fe-TMPyP, a representative peroxynitrite decomposition catalyst (PDC), remarkably reduced level of ONOO(-) and 3-NT and ameliorated the serum ALT, AST and TNF-α levels in both wild-type and Cav-1(-/-) mice. Taken together, we conclude that Cav-1 could play a critical role in preventing nitrative stress-induced liver damage during hepatic ischemia-reperfusion injury. PMID:27021966

  15. Caveolin-1 and CDC42 mediated endocytosis of silica-coated iron oxide nanoparticles in HeLa cells

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    Nils Bohmer

    2015-01-01

    Full Text Available Nanomedicine is a rapidly growing field in nanotechnology, which has great potential in the development of new therapies for numerous diseases. For example iron oxide nanoparticles are in clinical use already in the thermotherapy of brain cancer. Although it has been shown, that tumor cells take up these particles in vitro, little is known about the internalization routes. Understanding of the underlying uptake mechanisms would be very useful for faster and precise development of nanoparticles for clinical applications. This study aims at the identification of key proteins, which are crucial for the active uptake of iron oxide nanoparticles by HeLa cells (human cervical cancer as a model cell line. Cells were transfected with specific siRNAs against Caveolin-1, Dynamin 2, Flotillin-1, Clathrin, PIP5Kα and CDC42. Knockdown of Caveolin-1 reduces endocytosis of superparamagnetic iron oxide nanoparticles (SPIONs and silica-coated iron oxide nanoparticles (SCIONs between 23 and 41%, depending on the surface characteristics of the nanoparticles and the experimental design. Knockdown of CDC42 showed a 46% decrease of the internalization of PEGylated SPIONs within 24 h incubation time. Knockdown of Dynamin 2, Flotillin-1, Clathrin and PIP5Kα caused no or only minor effects. Hence endocytosis in HeLa cells of iron oxide nanoparticles, used in this study, is mainly mediated by Caveolin-1 and CDC42. It is shown here for the first time, which proteins of the endocytotic pathway mediate the endocytosis of silica-coated iron oxide nanoparticles in HeLa cells in vitro. In future studies more experiments should be carried out with different cell lines and other well-defined nanoparticle species to elucidate possible general principles.

  16. Caveolin-1 Deficiency Induces Spontaneous Endothelial-to-Mesenchymal Transition in Murine Pulmonary Endothelial Cells in Vitro

    OpenAIRE

    Li, Zhaodong; Wermuth, Peter J.; Benn, Bryan S.; Lisanti, Michael P.; Jimenez, Sergio A.

    2013-01-01

    It was previously demonstrated that transforming growth factor β (TGF-β) induces endothelial-to-mesenchymal transition (EndoMT) in murine lung endothelial cells (ECs) in vitro. Owing to the important role of caveolin-1 (CAV1) in TGF-β receptor internalization and TGF-β signaling, the participation of CAV1 in the induction of EndoMT in murine lung ECs was investigated. Pulmonary ECs were isolated from wild-type and Cav1 knockout mice using immunomagnetic methods with sequential anti-CD31 and a...

  17. Enhancing Myoblast Fusion for Therapy of Muscular Dystrophies

    OpenAIRE

    Wu, Melissa P.

    2013-01-01

    Skeletal muscle is a major organ comprising 30-40% of the human body mass. The coordination of processes resulting in mature muscle requires many genes, and their loss can result in debilitating muscle disorders. Of the strategies being developed to cure muscle diseases, enhancement of the natural process of muscle cell fusion in existing or introduced myogenic cells has great therapeutic potential. In this work, we determined whether a drug that stimulates proliferation and fusion of myoblas...

  18. Fusion yield enhancement in magnetized laser-driven implosions.

    Science.gov (United States)

    Chang, P Y; Fiksel, G; Hohenberger, M; Knauer, J P; Betti, R; Marshall, F J; Meyerhofer, D D; Séguin, F H; Petrasso, R D

    2011-07-15

    Enhancement of the ion temperature and fusion yield has been observed in magnetized laser-driven inertial confinement fusion implosions on the OMEGA Laser Facility. A spherical CH target with a 10 atm D2 gas fill was imploded in a polar-drive configuration. A magnetic field of 80 kG was embedded in the target and was subsequently trapped and compressed by the imploding conductive plasma. As a result of the hot-spot magnetization, the electron radial heat losses were suppressed and the observed ion temperature and neutron yield were enhanced by 15% and 30%, respectively. PMID:21838372

  19. A sensitive HIV-1 envelope induced fusion assay identifies fusion enhancement of thrombin

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, De-Chun; Zhong, Guo-Cai; Su, Ju-Xiang [Department of Microbiology, Harbin Medical University, 194 Xuefu Road, Harbin, Heilongjiang 150081 (China); Liu, Yan-Hong [Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Harbin, Heilongjiang 150081 (China); Li, Yan; Wang, Jia-Ye [Department of Microbiology, Harbin Medical University, 194 Xuefu Road, Harbin, Heilongjiang 150081 (China); Hattori, Toshio [Department of Emerging Infectious Diseases, Division of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai 9808574 (Japan); Ling, Hong, E-mail: lingh@ems.hrbmu.edu.cn [Department of Microbiology, Harbin Medical University, 194 Xuefu Road, Harbin, Heilongjiang 150081 (China); Department of Parasitology, Harbin Medical University, 194 Xuefu Road, Harbin, Heilongjiang 150081 (China); Key Lab of Heilongjiang Province for Infection and Immunity, Key Lab of Heilongjiang Province Education Bureau for Etiology, Harbin, Heilongjiang 150081 (China); Zhang, Feng-Min, E-mail: fengminzhang@yahoo.com.cn [Department of Microbiology, Harbin Medical University, 194 Xuefu Road, Harbin, Heilongjiang 150081 (China); Key Lab of Heilongjiang Province for Infection and Immunity, Key Lab of Heilongjiang Province Education Bureau for Etiology, Harbin, Heilongjiang 150081 (China)

    2010-01-22

    To evaluate the interaction between HIV-1 envelope glycoprotein (Env) and target cell receptors, various cell-cell-fusion assays have been developed. In the present study, we established a novel fusion system. In this system, the expression of the sensitive reporter gene, firefly luciferase (FL) gene, in the target cells was used to evaluate cell fusion event. Simultaneously, constitutively expressed Renilla luciferase (RL) gene was used to monitor effector cell number and viability. FL gave a wider dynamic range than other known reporters and the introduction of RL made the assay accurate and reproducible. This system is especially beneficial for investigation of potential entry-influencing agents, for its power of ruling out the false inhibition or enhancement caused by the artificial cell-number variation. As a case study, we applied this fusion system to observe the effect of a serine protease, thrombin, on HIV Env-mediated cell-cell fusion and have found the fusion enhancement activity of thrombin over two R5-tropic HIV strains.

  20. A sensitive HIV-1 envelope induced fusion assay identifies fusion enhancement of thrombin

    International Nuclear Information System (INIS)

    To evaluate the interaction between HIV-1 envelope glycoprotein (Env) and target cell receptors, various cell-cell-fusion assays have been developed. In the present study, we established a novel fusion system. In this system, the expression of the sensitive reporter gene, firefly luciferase (FL) gene, in the target cells was used to evaluate cell fusion event. Simultaneously, constitutively expressed Renilla luciferase (RL) gene was used to monitor effector cell number and viability. FL gave a wider dynamic range than other known reporters and the introduction of RL made the assay accurate and reproducible. This system is especially beneficial for investigation of potential entry-influencing agents, for its power of ruling out the false inhibition or enhancement caused by the artificial cell-number variation. As a case study, we applied this fusion system to observe the effect of a serine protease, thrombin, on HIV Env-mediated cell-cell fusion and have found the fusion enhancement activity of thrombin over two R5-tropic HIV strains.

  1. Fusion Enhancement for Neutron-Rich Light Nuclei

    CERN Document Server

    Singh, Varinderjit; Steinbach, T K; Hudan, S; deSouza, R T; Baby, L T; Kuvin, S A; Tripathi, V; Wiedenhover, I

    2016-01-01

    Measurement of the fusion cross-section for neutron-rich light nuclei is crucial in ascertaining if fusion of these nuclei occurs in the outer crust of a neutron star. The fusion excitation function at near-barrier energies for the $^{19}$O + $^{12}$C system was measured and the experimental results are compared to the fusion excitation function of $^{18}$O + $^{12}$C and $^{16}$O + $^{12}$C. The experiment was performed by utilizing a beam of $^{19}$O, produced via the $^{18}$O(d,p) reaction, to bombard a $^{12}$C target at energies near the Coulomb barrier. Evaporation residues produced in fusion of $^{18,19}$O ions with $^{12}$C target nuclei were detected with good geometric efficiency and identified by measuring their energy and time-of-flight. A significant enhancement is observed in the fusion probability of $^{19}$O ions with a $^{12}$C target as compared to $^{18}$O ions. The larger cross-sections observed at near barrier energies is related to significant narrowing of the fusion barrier indicating a...

  2. IMAGE ENHANCEMENT USING IMAGE FUSION AND IMAGE PROCESSING TECHNIQUES

    OpenAIRE

    Arjun Nelikanti

    2015-01-01

    Principle objective of Image enhancement is to process an image so that result is more suitable than original image for specific application. Digital image enhancement techniques provide a multitude of choices for improving the visual quality of images. Appropriate choice of such techniques is greatly influenced by the imaging modality, task at hand and viewing conditions. This paper will provide a combination of two concepts, image fusion by DWT and digital image processing techniques. The e...

  3. Roxithromycin suppresses airway remodeling and modulates the expression of caveolin-1 and phospho-p42/p44MAPK in asthmatic rats.

    Science.gov (United States)

    Wu, Li-Qin; Wang, Rui-Li; Dai, Yuan-Rong; Li, Feng-Qin; Wu, Hai-Ya; Yan, Sun-Shun; Wang, Liang-Rong; Jin, Li-da; Xia, Xiao-Dong

    2015-02-01

    Roxithromycin (RXM) expresses anti-asthmatic effects that are separate from its antibiotic activity, but its effects on airway remodeling are still unknown. Here, we evaluated the effects of RXM on airway remodeling and the expression of caveolin-1 and phospho-p42/p44mitogen-activated protein kinase (phospho-p42/p44MAPK) in chronic asthmatic rats. The chronic asthma was induced by ovalbumin/Al(OH)3 sensitization and ovalbumin challenge, RXM (30mg/kg) or dexamethasone (0.5mg/kg) was given before airway challenge initiation. We measured the thickness of bronchial wall and bronchial smooth muscle cell layer to indicate airway remodeling, and caveolin-1 and phospho-p42/p44MAPK expression in lung tissue and airway smooth muscle were detected by immunohistochemistry and western blot analysis, respectively. The results demonstrated that RXM treatment decreased the thickness of bronchial wall and bronchial smooth muscle cell layer, and also downregulated the phospho-p42/p44MAPK expression and upregulated the caveolin-1 expression. The above effects of RXM were similar to dexamethasone. Our results suggested that pretreatment with RXM could suppress airway remodeling and regulate the expression of caveolin-1 and phospho-p42/p44MAPK in chronic asthmatic rats. PMID:25479721

  4. Static pressure accelerates ox-LDL-induced cholesterol accumulation via SREBP-1-mediated caveolin-1 downregulation in cultured vascular smooth muscle cells

    International Nuclear Information System (INIS)

    Research highlights: → Vertical static pressure accelerates ox-LDL-induced cholesterol accumulation in cultured vascular smooth muscle cells. → Static pressure induces SREBP-1 activation. → Static pressure downregulates the expressions of caveolin-1 by activating SREBP-1. → Static pressure also downregulates the transcription of ABCA1 by activating SREBP-1. → Static pressure increases ox-LDL-induced cholesterol accumulation by SREBP-1-mediated caveolin-1 downregulation in vascular smooth muscle cells cultured in vitro. -- Abstract: Objective: To investigate the effect of static pressure on cholesterol accumulation in vascular smooth muscle cells (VSMCs) and its mechanism. Methods: Rat-derived VSMC cell line A10 treated with 50 mg/L ox-LDL and different static pressures (0, 60, 90, 120, 150, 180 mm Hg) in a custom-made pressure incubator for 48 h. Intracellular lipid droplets and lipid levels were assayed by oil red O staining and HPLC; The mRNA levels of caveolin-1 and ABCA1, the protein levels of caveolin-1 SREBP-1 and mature SREBP-1 were respectively detected by RT-PCR or western blot. ALLN, an inhibitor of SREBP metabolism, was used to elevate SREBP-1 protein level in VSMCs treated with static pressure. Results: Static pressures significantly not only increase intracellular lipid droplets in VSMCs, but also elevate cellular lipid content in a pressure-dependent manner. Intracellular free cholesterol (FC), cholesterol ester (CE), total cholesterol (TC) were respectively increased from 60.5 ± 2.8 mg/g, 31.8 ± 0.7 mg/g, 92.3 ± 2.1 mg/g at atmosphere pressure (ATM, 0 mm Hg) to 150.8 ± 9.4 mg/g, 235.9 ± 3.0 mg/g, 386.7 ± 6.4 mg/g at 180 mm Hg. At the same time, static pressures decrease the mRNA and protein levels of caveolin-1, and induce the activation and nuclear translocation of SREBP-1. ALLN increases the protein level of mature SREBP-1 and decreases caveolin-1 expression, so that cellular lipid levels were upregulated. Conclusion: Static

  5. Image Enhancement by Image Fusion for Crime Investigation

    Directory of Open Access Journals (Sweden)

    S. Kanaga Suba Raja

    2016-06-01

    Full Text Available In the criminal investigation field, images are the principal forms for investigation and for probing crime detection. The imaging science applied in criminal investigation is face detection, surveillance camera imaging, and crime scene analysis. Digital imaging succors image manipulation, alteration and enhancement techniques. The traditional methodologies enhance the given image by improving the local or global components of the image. It proves a debacle since it engages noise amplification, block discontinuities, colour mismatch, edge distortion and checkerboard effects thereby limiting image processing tasks. To the same degree of enhancement, spurned artefacts are given rise. Thus to balance the global and local factors of the image and to weed out the tenebrous components; fusion of multiple alike images are performed to produce a meliorated image. The fusion is done by fusing a pyramid constructed image and a wavelet transformed image. The pyramid image and the wavelet transformed image are then fused through to afford a revealing image for better perception by the human visual system. The experimental results show that our proposed fusion scheme is effective and the fusion is applied over a surveillance camera image grab.

  6. Metronomic Ceramide Analogs Inhibit Angiogenesis in Pancreatic Cancer through Up-regulation of Caveolin-1 and Thrombospondin-1 and Down-regulation of Cyclin D1

    Directory of Open Access Journals (Sweden)

    Guido Bocci

    2012-09-01

    Full Text Available AIMS: To evaluate the antitumor and antiangiogenic activity of metronomic ceramide analogs and their relevant molecular mechanisms. METHODS: Human endothelial cells [human dermal microvascular endothelial cells and human umbilical vascular endothelial cell (HUVEC] and pancreatic cancer cells (Capan-1 and MIA PaCa-2 were treated with the ceramide analogs (C2, AL6, C6, and C8, at low concentrations for 144 hours to evaluate any antiproliferative and proapoptotic effects and inhibition of migration and to measure the expression of caveolin-1 (CAV-1 and thrombospondin-1 (TSP-1 mRNAs by real-time reverse transcription-polymerase chain reaction. Assessment of extracellular signal-regulated kinases 1 and 2 (ERK1/2 and Akt phosphorylation and of CAV-1 and cyclin D1 protein expression was performed by ELISA. Maximum tolerated dose (MTD gemcitabine was compared against metronomic doses of the ceramide analogs by evaluating the inhibition of MIA PaCa-2 subcutaneous tumor growth in nude mice. RESULTS: Metronomic ceramide analogs preferentially inhibited cell proliferation and enhanced apoptosis in endothelial cells. Low concentrations of AL6 and C2 caused a significant inhibition of HUVEC migration. ERK1/2 and Akt phosphorylation were significantly decreased after metronomic ceramide analog treatment. Such treatment caused the overexpression of CAV-1 and TSP-1 mRNAs and proteins in endothelial cells, whereas cyclin D1 protein levels were reduced. The antiangiogenic and antitumor impact in vivo of metronomic C2 and AL6 regimens was similar to that caused by MTD gemcitabine. CONCLUSIONS: Metronomic C2 and AL6 analogs have antitumor and antiangiogenic activity, determining the up-regulation of CAV-1 and TSP-1 and the suppression of cyclin D1.

  7. Oral cancer/endothelial cell fusion experiences nuclear fusion and acquisition of enhanced survival potential

    Energy Technology Data Exchange (ETDEWEB)

    Song, Kai [Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Shandong Province (China); The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China); Song, Yong [The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China); Department of Stomatology, Liu Zhou People' s Hospital, Guangxi (China); Zhao, Xiao-Ping; Shen, Hui; Wang, Meng; Yan, Ting-lin [The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China); Liu, Ke, E-mail: liuke.1999@aliyun.com [Department of Oral and Maxillofacial-Head and Neck oncology, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079 (China); The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China); Shang, Zheng-jun, E-mail: shangzhengjun@hotmail.com [Department of Oral and Maxillofacial-Head and Neck oncology, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079 (China); The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University, Wuhan (China)

    2014-10-15

    Most previous studies have linked cancer–macrophage fusion with tumor progression and metastasis. However, the characteristics of hybrid cells derived from oral cancer and endothelial cells and their involvement in cancer remained unknown. Double-immunofluorescent staining and fluorescent in situ hybridization (FISH) were performed to confirm spontaneous cell fusion between eGFP-labeled human umbilical vein endothelial cells (HUVECs) and RFP-labeled SCC9, and to detect the expression of vementin and cytokeratin 18 in the hybrids. The property of chemo-resistance of such hybrids was examined by TUNEL assay. The hybrid cells in xenografted tumor were identified by FISH and GFP/RFP dual-immunofluoresence staining. We showed that SCC9 cells spontaneously fused with cocultured endothelial cells, and the resultant hybrid cells maintained the division and proliferation activity after re-plating and thawing. Such hybrids expressed markers of both parental cells and became more resistant to chemotherapeutic drug cisplatin as compared to the parental SCC9 cells. Our in vivo data indicated that the hybrid cells contributed to tumor composition by using of immunostaining and FISH analysis, even though the hybrid cells and SCC9 cells were mixed with 1:10,000, according to the FACS data. Our study suggested that the fusion events between oral cancer and endothelial cells undergo nuclear fusion and acquire a new property of drug resistance and consequently enhanced survival potential. These experimental findings provide further supportive evidence for the theory that cell fusion is involved in cancer progression. - Highlights: • The fusion events between oral cancer and endothelial cells undergo nuclear fusion. • The resulting hybrid cells acquire a new property of drug resistance. • The resulting hybrid cells express the markers of both parental cells (i.e. vimentin and cytokeratin 18). • The hybrid cells contribute to tumor repopulation in vivo.

  8. Oral cancer/endothelial cell fusion experiences nuclear fusion and acquisition of enhanced survival potential

    International Nuclear Information System (INIS)

    Most previous studies have linked cancer–macrophage fusion with tumor progression and metastasis. However, the characteristics of hybrid cells derived from oral cancer and endothelial cells and their involvement in cancer remained unknown. Double-immunofluorescent staining and fluorescent in situ hybridization (FISH) were performed to confirm spontaneous cell fusion between eGFP-labeled human umbilical vein endothelial cells (HUVECs) and RFP-labeled SCC9, and to detect the expression of vementin and cytokeratin 18 in the hybrids. The property of chemo-resistance of such hybrids was examined by TUNEL assay. The hybrid cells in xenografted tumor were identified by FISH and GFP/RFP dual-immunofluoresence staining. We showed that SCC9 cells spontaneously fused with cocultured endothelial cells, and the resultant hybrid cells maintained the division and proliferation activity after re-plating and thawing. Such hybrids expressed markers of both parental cells and became more resistant to chemotherapeutic drug cisplatin as compared to the parental SCC9 cells. Our in vivo data indicated that the hybrid cells contributed to tumor composition by using of immunostaining and FISH analysis, even though the hybrid cells and SCC9 cells were mixed with 1:10,000, according to the FACS data. Our study suggested that the fusion events between oral cancer and endothelial cells undergo nuclear fusion and acquire a new property of drug resistance and consequently enhanced survival potential. These experimental findings provide further supportive evidence for the theory that cell fusion is involved in cancer progression. - Highlights: • The fusion events between oral cancer and endothelial cells undergo nuclear fusion. • The resulting hybrid cells acquire a new property of drug resistance. • The resulting hybrid cells express the markers of both parental cells (i.e. vimentin and cytokeratin 18). • The hybrid cells contribute to tumor repopulation in vivo

  9. Image fusion for dynamic contrast enhanced magnetic resonance imaging

    Directory of Open Access Journals (Sweden)

    Leach Martin O

    2004-10-01

    Full Text Available Abstract Background Multivariate imaging techniques such as dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI have been shown to provide valuable information for medical diagnosis. Even though these techniques provide new information, integrating and evaluating the much wider range of information is a challenging task for the human observer. This task may be assisted with the use of image fusion algorithms. Methods In this paper, image fusion based on Kernel Principal Component Analysis (KPCA is proposed for the first time. It is demonstrated that a priori knowledge about the data domain can be easily incorporated into the parametrisation of the KPCA, leading to task-oriented visualisations of the multivariate data. The results of the fusion process are compared with those of the well-known and established standard linear Principal Component Analysis (PCA by means of temporal sequences of 3D MRI volumes from six patients who took part in a breast cancer screening study. Results The PCA and KPCA algorithms are able to integrate information from a sequence of MRI volumes into informative gray value or colour images. By incorporating a priori knowledge, the fusion process can be automated and optimised in order to visualise suspicious lesions with high contrast to normal tissue. Conclusion Our machine learning based image fusion approach maps the full signal space of a temporal DCE-MRI sequence to a single meaningful visualisation with good tissue/lesion contrast and thus supports the radiologist during manual image evaluation.

  10. The Inhibition Effect of Caveolin-1 on PANC1Human Pancreatic Tumor Growth In vitro and In vivo%Caveolin-1抑制胰腺癌细胞PANC1的体内外生长和增殖

    Institute of Scientific and Technical Information of China (English)

    王晓辉; 郑亚民; 崔叶青; 刘爽; 孙海晨; 李非

    2011-01-01

    窖蛋白-1(caveolin-1)是胞膜窖(caveolae)中重要的结构和功能蛋白.Caveolin-1参与细胞的多种生命活动并与恶性肿瘤的发生相关.为探讨caveolin-1对胰腺癌细胞PANC1的体外增殖、迁移、侵袭以及裸鼠体内成瘤能力的影响,通过基因转染技术培育caveolin-1过表达细胞株PANC1/cav-1作为实验组,转染空载体细胞株PANC 1/vector作为对照组,采用RT-PCR及Western blot方法检测caveolin-1的表达量,流式细胞术分析细胞周期,软琼脂细胞克隆实验检测细胞增殖能力,侵袭小室实验检测癌细胞迁移和侵袭的能力,建立裸鼠皮下种植瘤模型并检测肿瘤组织的增殖与凋亡.PANC1/cav-1中的caveolin-1表达稳定,表达量明显高于对照组细胞株和亲本细胞株(P<0.01),细胞周期检测显示大量PANC1/cav-1细胞被抑制于G0/Gl期,caveolin-1抑制PANC1的增殖,迁移和侵袭能力.在裸鼠的体内实验中,caveolin-1显著抑制PANC1细胞在裸鼠体内的生长,Ki-67染色和TUNEL染色表明在PANC1细胞中过表达caveolin-1,可以抑制肿瘤增殖并诱导肿瘤凋亡.上述结果表明,caveolin-1可能通过对胰腺癌细胞周期的影响(抑制于G0/G1期),抑制胰腺癌PANCl细胞在体内外的增殖、迁移和侵袭,并导致肿瘤凋亡.%Caveolin-1 is a transmembrane protein and essential structural constituent of the caveolae membrane.Caveolin-1 has been involved in multiple cellular functions and oncogenesis.To investigate the roles of caveolin-l,stable transfectants were established in PANC1 pancreatic adenocarcinoma cells which had up-regulated caveolin-1 expression.The plasmid pCI-neo-cav-1 and its corresponding empty vector (pCI-neo) were transfected into PANC 1 cell lines.The expression of caveolin-1 in these three cell lines was determined by RT-PCR and Western blot.Cell cycle phase distribution was determined by flow cytometry.The colony formation ability of tumor cells was detected by anchorage-independent growth

  11. ULTRASOUND INDUCED AND LASER ENHANCED COLD FUSION CHEMISTRY

    Institute of Scientific and Technical Information of China (English)

    T.V.Prevenslik

    1995-01-01

    The standard model of sonoluminescence suggests that the coulomb barrer to deuterium fusion may be overcome by high bubble gas temperatures caused by compression heating if the bubble diameter remains spherical during bubble collapse.However,in the more likely collapse geometry of a pancake shape,the temperature rise in the bubbles is negligible.But the collapsing pancake bubble is fund to significantly increase the frequency of the infrared energy available in the vibrational state of the water molecules at ambient temperature.For a collapse to liquied density,ultraviolet radiation at about 10eV is fund.Although the ultraviolet radiation is of a low intensity,higher intensities may be possible if the bubble collapse is enhanced by visible and infrared lases.Neither hot nor cold fusion is predicted in bubble collapse but the ultraviolet energy at about 10eV developed in the bubble is sufficient to provide the basis for a new field of chemistry called ultrasound induced and laser enhanced cold fusion chemistry.

  12. Tyrosine-phosphorylated caveolin-1 blocks bacterial uptake by inducing Vav2-RhoA-mediated cytoskeletal rearrangements.

    Directory of Open Access Journals (Sweden)

    Jan Peter Boettcher

    Full Text Available Certain bacterial adhesins appear to promote a pathogen's extracellular lifestyle rather than its entry into host cells. However, little is known about the stimuli elicited upon such pathogen host-cell interactions. Here, we report that type IV pili (Tfp-producing Neisseria gonorrhoeae (P(+GC induces an immediate recruitment of caveolin-1 (Cav1 in the host cell, which subsequently prevents bacterial internalization by triggering cytoskeletal rearrangements via downstream phosphotyrosine signaling. A broad and unbiased analysis of potential interaction partners for tyrosine-phosphorylated Cav1 revealed a direct interaction with the Rho-family guanine nucleotide exchange factor Vav2. Both Vav2 and its substrate, the small GTPase RhoA, were found to play a direct role in the Cav1-mediated prevention of bacterial uptake. Our findings, which have been extended to enteropathogenic Escherichia coli, highlight how Tfp-producing bacteria avoid host cell uptake. Further, our data establish a mechanistic link between Cav1 phosphorylation and pathogen-induced cytoskeleton reorganization and advance our understanding of caveolin function.

  13. The Importance of Caveolin-1 as Key-Regulator of Three-Dimensional Growth in Thyroid Cancer Cells Cultured under Real and Simulated Microgravity Conditions

    OpenAIRE

    Stefan Riwaldt; Johann Bauer; Jessica Pietsch; Markus Braun; Jürgen Segerer; Achim Schwarzwälder; Corydon, Thomas J.; Manfred Infanger; Daniela Grimm

    2015-01-01

    We recently demonstrated that the CAV1 gene was down-regulated, when poorly differentiated thyroid FTC-133 cancer cells formed spheroids under simulated microgravity conditions. Here, we present evidence that the caveolin-1 protein is involved in the inhibition of spheroid formation, when confluent monolayers are exposed to microgravity. The evidence is based on proteins detected in cells and their supernatants of the recent spaceflight experiment: “NanoRacks-CellBox-Thyroid Cancer”. The cult...

  14. Enhanced Charged Higgs Production through W^\\pm-Higgs Fusion

    CERN Document Server

    Arhrib, Abdesslam; Lee, Jae Sik; Lu, Chih-Ting

    2015-01-01

    We study the associated production of a charged Higgs boson with a bottom quark and a light quark at the LHC via p p \\to H^\\pm\\,b\\,j in the Two Higgs Doublet Models (2HDMs). Using the effective W approximation, we show that there is exact cancellation among various Feynman diagrams in high energy limit. This may imply that the production of charged Higgs can be significantly enhanced in the presence of large mass differences among the neutral Higgs bosons via W^\\pm-Higgs fusion in the p p \\to H^\\pm\\,b\\,j process. Particularly, we emphasize the potential enhancement due to a light pseudoscalar boson $A$, which is still allowed by the current data by which we explicitly calculate the allowed regions in (M_A,\\,\\tan\\beta) plane, and show that the production cross section can be as large as 0.1 pb for large $\\tan\\beta$. We also show that the transverse momentum distribution of the b quark can potentially distinguish the W^\\pm-A fusion diagram from the top diagram. Finally, we point out further enhancement when we ...

  15. Roles of Caveolin-1 in Angiotensin II-Induced Hypertrophy and Inward Remodeling of Cerebral Pial Arterioles.

    Science.gov (United States)

    Umesalma, Shaikamjad; Houwen, Frederick Keith; Baumbach, Gary L; Chan, Siu-Lung

    2016-03-01

    Angiotensin II (Ang II) is a major determinant of inward remodeling and hypertrophy in pial arterioles that may have an important role in stroke during chronic hypertension. Previously, we found that epidermal growth factor receptor is critical in Ang II-mediated hypertrophy that may involve caveolin-1 (Cav-1). In this study, we examined the effects of Cav-1 and matrix metalloproteinase-9 (MMP9) on Ang II-mediated structural changes in pial arterioles. Cav-1-deficient (Cav-1(-/-)), MMP9-deficient (MMP9(-/-)), and wild-type mice were infused with either Ang II (1000 ng/kg per minute) or saline via osmotic minipumps for 28 days (n=6-8 per group). Systolic arterial pressure was measured by a tail-cuff method. Pressure and diameter of pial arterioles were measured through an open cranial window in anesthetized mice. Cross-sectional area of the wall was determined histologically in pressurized fixed pial arterioles. Expression of Cav-1, MMP9, phosphorylated epidermal growth factor receptor, and Akt was determined by Western blotting and immunohistochemistry. Deficiency of Cav-1 or MMP9 did not affect Ang II-induced hypertension. Ang II increased the expression of Cav-1, phosphorylated epidermal growth factor receptor, and Akt in wild-type mice, which was attenuated in Cav-1(-/-) mice. Ang II-induced hypertrophy, inward remodeling, and increased MMP9 expression in pial arterioles were prevented in Cav-1(-/-) mice. Ang II-mediated increases in MMP9 expression and inward remodeling, but not hypertrophy, were prevented in MMP9(-/-) mice. In conclusion, Cav-1 is essential in Ang II-mediated inward remodeling and hypertrophy in pial arterioles. Cav-1-induced MMP9 is exclusively involved in inward remodeling, not hypertrophy. Further studies are needed to determine the role of Akt in Ang II-mediated hypertrophy. PMID:26831194

  16. Integrin α1β1 Promotes Caveolin-1 Dephosphorylation by Activating T Cell Protein-tyrosine Phosphatase*

    Science.gov (United States)

    Borza, Corina M.; Chen, Xiwu; Mathew, Sijo; Mont, Stacey; Sanders, Charles R.; Zent, Roy; Pozzi, Ambra

    2010-01-01

    Integrin α1β1 is a collagen receptor that down-regulates collagen and reactive oxygen species (ROS) production, and mice lacking this receptor show increased ROS levels and exacerbated glomerular sclerosis following injury. Caveolin-1 (Cav-1) is a multifunctional protein that is tyrosine-phosphorylated in response to injury and has been implicated in ROS-mediated injury. Cav-1 interacts with integrins, and integrin α1β1 binds/activates T cell protein-tyrosine phosphatase (TCPTP), which is homologous to the tyrosine phosphatase PTP1B known to dephosphorylate Cav-1. In this study, we analyzed whether phosphorylated Cav-1 (pCav-1) is a substrate of TCPTP and if integrin α1β1 is essential for promoting TCPTP-mediated Cav-1 dephosphorylation. We found that Cav-1 phosphorylation is significantly higher in cells lacking integrin α1β1 at base line and following oxidative stress. Overexpression of TCPTP leads to reduced pCav-1 levels only in cells expressing integrin α1β1. Using solid phase binding assays, we demonstrated that 1) purified Cav-1 directly interacts with TCPTP and the integrin α1 subunit, 2) pCav-1 is a substrate of TCPTP, and 3) TCPTP-mediated Cav-1 dephosphorylation is highly increased by the addition of purified integrin α1β1 or an integrin α1 cytoplasmic peptide to which TCPTP has been shown to bind. Thus, our results demonstrate that pCav-1 is a new substrate of TCPTP and that integrin α1β1 acts as a negative regulator of Cav-1 phosphorylation by activating TCPTP. This could explain the protective function of integrin α1β1 in oxidative stress-mediated damage and why integrin α1-null mice are more susceptible to fibrosis following injury. PMID:20940300

  17. Integrin {alpha}1{beta}1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase.

    Science.gov (United States)

    Borza, Corina M; Chen, Xiwu; Mathew, Sijo; Mont, Stacey; Sanders, Charles R; Zent, Roy; Pozzi, Ambra

    2010-12-17

    Integrin α1β1 is a collagen receptor that down-regulates collagen and reactive oxygen species (ROS) production, and mice lacking this receptor show increased ROS levels and exacerbated glomerular sclerosis following injury. Caveolin-1 (Cav-1) is a multifunctional protein that is tyrosine-phosphorylated in response to injury and has been implicated in ROS-mediated injury. Cav-1 interacts with integrins, and integrin α1β1 binds/activates T cell protein-tyrosine phosphatase (TCPTP), which is homologous to the tyrosine phosphatase PTP1B known to dephosphorylate Cav-1. In this study, we analyzed whether phosphorylated Cav-1 (pCav-1) is a substrate of TCPTP and if integrin α1β1 is essential for promoting TCPTP-mediated Cav-1 dephosphorylation. We found that Cav-1 phosphorylation is significantly higher in cells lacking integrin α1β1 at base line and following oxidative stress. Overexpression of TCPTP leads to reduced pCav-1 levels only in cells expressing integrin α1β1. Using solid phase binding assays, we demonstrated that 1) purified Cav-1 directly interacts with TCPTP and the integrin α1 subunit, 2) pCav-1 is a substrate of TCPTP, and 3) TCPTP-mediated Cav-1 dephosphorylation is highly increased by the addition of purified integrin α1β1 or an integrin α1 cytoplasmic peptide to which TCPTP has been shown to bind. Thus, our results demonstrate that pCav-1 is a new substrate of TCPTP and that integrin α1β1 acts as a negative regulator of Cav-1 phosphorylation by activating TCPTP. This could explain the protective function of integrin α1β1 in oxidative stress-mediated damage and why integrin α1-null mice are more susceptible to fibrosis following injury. PMID:20940300

  18. Interaction of caveolin-1, nitric oxide, and nitric oxide synthases in hypoxic human SK-N-MC neuroblastoma cells.

    Science.gov (United States)

    Shen, Jiangang; Lee, Waisin; Li, Yue; Lau, Chi Fai; Ng, Kwong Man; Fung, Man Lung; Liu, Ke Jian

    2008-10-01

    Neuroblastoma cells are capable of hypoxic adaptation, but the mechanisms involved are not fully understood. We hypothesized that caveolin-1 (cav-1), a plasma membrane signal molecule, might play a role in protecting neuroblastoma cells from oxidative injury by modulating nitric oxide (NO) production. We investigated the alterations of cav-1, cav-2, nitric oxide synthases (NOS), and NO levels in human SK-N-MC neuroblastoma cells exposed to hypoxia with 2% [O2]. The major discoveries include: (i) cav-1 but not cav-2 was up-regulated in the cells exposed to 15 h of hypoxia; (ii) NO donor 1-[N, N-di-(2-aminoethyl) amino] diazen-1-ium-1, 2-diolate up-regulated the expression of cav-1, whereas the non-selective NOS inhibitor N(G)-nitro-L-arginine methyl ester and inducible NOS (iNOS) inhibitor 1400W each abolished the increase in cav-1 expression in the hypoxic SK-N-MC cells. These results suggest that iNOS-induced NO production contributes to the up-regulation of cav-1 in the hypoxic SK-N-MC cells. Furthermore, we studied the roles played by cav-1 in regulating NO, NOS, and apoptotic cell death in the SK-N-MC cells subjected to 15 h of hypoxic treatment. Both cav-1 transfection and cav-1 scaffolding domain peptide abolished the induction of iNOS, reduced the production of NO, and reduced the rates of apoptotic cell death in the hypoxic SK-N-MC cells. These results suggest that increased expression of cav-1 in response to hypoxic stimulation could prevent oxidative injury induced by reactive oxygen species. The interactions of cav-1, NO, and NOS could be an important signal pathway in protecting the neuroblastoma cells from oxidative injury, contributing to the hypoxic tolerance of neuroblastoma cells. PMID:18717816

  19. Curcumin attenuates high glucose-induced podocyte apoptosis by regulating functional connections between caveolin-1 phosphorylation and ROS

    Science.gov (United States)

    Sun, Li-na; Liu, Xiang-chun; Chen, Xiang-jun; Guan, Guang-ju; Liu, Gang

    2016-01-01

    Aim: Caveolin-1 (cav-1) is a major multifunctional scaffolding protein of caveolae. Cav-1 is primarily expressed in mesangial cells, renal proximal tubule cells and podocytes in kidneys. Recent evidence shows that the functional connections between cav-1 and ROS play a key role in many diseases. In this study we investigated whether regulating the functional connections between cav-1 and ROS in kidneys contributed to the beneficial effects of curcumin in treating diabetic nephropathy in vitro and in vivo. Methods: Cultured mouse podocytes (mpc5) were incubated in a high glucose (HG, 30 mmol/L) medium for 24, 48 or 72 h. Male rats were injected with STZ (60 mg/kg, ip) to induce diabetes. ROS generation, SOD activity, MDA content and caspase-3 activity in the cultured cells and kidney cortex homogenate were determined. Apoptotic proteins and cav-1 phosphorylation were analyzed using Western blot analyses. Results: Incubation in HG-containing medium time-dependently increased ROS production, oxidative stress, apoptosis, and cav-1 phosphorylation in podocytes. Pretreatment with curcumin (1, 5, and 10 μmol/L) dose-dependently attenuated these abnormalities in HG-treated podocytes. Furthermore, in HG-containing medium, the podocytes transfected with a recombinant plasmid GFP-cav-1 Y14F (mutation at a cav-1 phosphorylation site) exhibited significantly decreased ROS production and apoptosis compared with the cells transfected with empty vector. In diabetic rats, administration of curcumin (100 or 200 mg/kg body weight per day, ig, for 8 weeks) not only significantly improved the renal function, but also suppressed ROS levels, oxidative stress, apoptosis and cav-1 phosphorylation in the kidneys. Conclusion: Curcumin attenuates high glucose-induced podocyte apoptosis in vitro and diabetic nephropathy in vivo partly through regulating the functional connections between cav-1 phosphorylation and ROS. PMID:26838071

  20. Feasibility of Reduced Tritium Circulation in the Heliotron Reactor by Enhancing Fusion Reactivity Using ICRF

    OpenAIRE

    Yanagi, Nagato; SHYSHKIN, Oleg A.; Goto, Takuya; Kasahara, Hiroshi; MIYAZAWA, Junichi; SAGARA, Akio

    2011-01-01

    A scheme for reducing the tritium fraction in DT fusion reactors is investigated by means of enhancing the fusion reactivity using high-power ICRF heating in heliotron reactors. We assume a situation that the density fraction of tritons is less than 10%, and the minority tritons are accelerated by ICRF waves. We then analyze the increase of fusion reactivity by assuming an effective temperature of high-energy tritons and examine the possibility of realizing a fusion reactor with this concept....

  1. COMBINING SCENE MODEL AND FUSION FOR NIGHT VIDEO ENHANCEMENT

    Institute of Scientific and Technical Information of China (English)

    Li Jing; Yang Tao; Pan Quan; Cheng Yongmei

    2009-01-01

    This paper presents a video context enhancement method for night surveillance. The basic idea is to extract and fuse the meaningful information of video sequence captured from a fixed camera under different illuminations. A unique characteristic of the algorithm is to separate the image context into two classes and estimate them in different ways. One class contains basic surrounding scene information and scene model, which is obtained via background modeling and object tracking in daytime video sequence. The other class is extracted from nighttime video, including frequently moving region, high illumination region and high gradient region. The scene model and pixel-wise difference method are used to segment the three regions. A shift-invariant discrete wavelet based image fusion technique is used to integral all those context information in the final result. Experiment results demonstrate that the proposed approach can provide much more details and meaningful information for nighttime video.

  2. Caveolin-1 Regulates the P2Y2 Receptor Signaling in Human 1321N1 Astrocytoma Cells.

    Science.gov (United States)

    Martinez, Namyr A; Ayala, Alondra M; Martinez, Magdiel; Martinez-Rivera, Freddyson J; Miranda, Jorge D; Silva, Walter I

    2016-06-01

    Damage to the CNS can cause a differential spatio-temporal release of multiple factors, such as nucleotides, ATP and UTP. The latter interact with neuronal and glial nucleotide receptors. The P2Y2 nucleotide receptor (P2Y2R) has gained prominence as a modulator of gliotic responses after CNS injury. Still, the molecular mechanisms underlying these responses in glia are not fully understood. Membrane-raft microdomains, such as caveolae, and their constituent caveolins, modulate receptor signaling in astrocytes; yet, their role in P2Y2R signaling has not been adequately explored. Hence, this study evaluated the role of caveolin-1 (Cav-1) in modulating P2Y2R subcellular distribution and signaling in human 1321N1 astrocytoma cells. Recombinant hP2Y2R expressed in 1321N1 cells and Cav-1 were found to co-fractionate in light-density membrane-raft fractions, co-localize via confocal microscopy, and co-immunoprecipitate. Raft localization was dependent on ATP stimulation and Cav-1 expression. This hP2Y2R/Cav-1 distribution and interaction was confirmed with various cell model systems differing in the expression of both P2Y2R and Cav-1, and shRNA knockdown of Cav-1 expression. Furthermore, shRNA knockdown of Cav-1 expression decreased nucleotide-induced increases in the intracellular Ca(2+) concentration in 1321N1 and C6 glioma cells without altering TRAP-6 and carbachol Ca(2+) responses. In addition, Cav-1 shRNA knockdown also decreased AKT phosphorylation and altered the kinetics of ERK1/2 activation in 1321N1 cells. Our findings strongly suggest that P2Y2R interaction with Cav-1 in membrane-raft caveolae of 1321N1 cells modulates receptor coupling to its downstream signaling machinery. Thus, P2Y2R/Cav-1 interactions represent a novel target for controlling P2Y2R function after CNS injury. PMID:27129210

  3. Effect of diet on expression of caveolin-1 in arteries of rats%膳食对大鼠动脉窖蛋白-1表达的影响

    Institute of Scientific and Technical Information of China (English)

    许明佳; 杨年红; 应晨江; 王重建; 刘烈刚; 孙秀发

    2006-01-01

    目的研究膳食脂类对大鼠动脉窖蛋白-1(caveolin-1)表达的影响.方法雄性SD大鼠试验组用高脂饲料喂养15周诱导肥胖(DIO)和肥胖抵抗(DIO-R)后,将DIO组一半改用基础饲料喂养(DIO-HF/LF),另一半继续高脂饮食(DIO-HF);对照组(CF)一直用基础饲料喂养.第23周末用RT-PCR、免疫印迹及免疫组化方法检测动脉血管caveolin-1表达水平.结果 DIO-HF/LF组体重低于DIO-HF组而高于对照组(P<0.05).血管caveolin-1水平显示,DIO-HF高于DIO- HF/LF、DIO-R及CF,而其余3组之间差异无统计学意义.结论基础饮食可降低肥胖敏感大鼠体重及血管caveolin-1表达,降低心血管疾病的危险性.肥胖抵抗可能与caveolin-1维持正常表达有关.

  4. Cooperative Role of Mineralocorticoid Receptor and Caveolin-1 in Regulating the Vascular Response to Low Nitric Oxide-High Angiotensin II-Induced Cardiovascular Injury.

    Science.gov (United States)

    Pojoga, Luminita H; Yao, Tham M; Opsasnick, Lauren A; Siddiqui, Waleed T; Reslan, Ossama M; Adler, Gail K; Williams, Gordon H; Khalil, Raouf A

    2015-10-01

    Aldosterone interacts with mineralocorticoid receptor (MR) to stimulate sodium reabsorption in renal tubules and may also affect the vasculature. Caveolin-1 (cav-1), an anchoring protein in plasmalemmal caveolae, binds steroid receptors and also endothelial nitric oxide synthase, thus limiting its translocation and activation. To test for potential MR/cav-1 interaction in the vasculature, we investigated if MR blockade in cav-1-replete or -deficient states would alter vascular function in a mouse model of low nitric oxide (NO)-high angiotensin II (AngII)-induced cardiovascular injury. Wild-type (WT) and cav-1 knockout mice (cav-1(-/-)) consuming a high salt diet (4% NaCl) received Nω-nitro-l-arginine methyl ester (L-NAME) (0.1-0.2 mg/ml in drinking water at days 1-11) plus AngII (0.7-2.8 mg/kg per day via an osmotic minipump at days 8-11) ± MR antagonist eplerenone (EPL) 100 mg/kg per day in food. In both genotypes, blood pressure increased with L-NAME + AngII. EPL minimally changed blood pressure, although its dose was sufficient to block MR and reverse cardiac expression of the injury markers cluster of differentiation 68 and plasminogen activator inhibitor-1 in L-NAME+AngII treated mice. In aortic rings, phenylephrine and KCl contraction was enhanced with EPL in L-NAME+AngII treated WT mice, but not cav-1(-/-) mice. AngII-induced contraction was not different, and angiotensin type 1 receptor expression was reduced in L-NAME + AngII treated WT and cav-1(-/-) mice. In WT mice, acetylcholine-induced relaxation was enhanced with L-NAME + AngII treatment and reversed with EPL. Acetylcholine relaxation in cav-1(-/-) mice was greater than in WT mice, not modified by L-NAME + AngII or EPL, and blocked by ex vivo L-NAME, 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one (ODQ), or endothelium removal, suggesting the role of NO-cGMP. Cardiac endothelial NO synthase was increased in cav-1(-/-) versus WT mice, further increased with L-NAME + AngII, and not affected by EPL

  5. The Importance of Caveolin-1 as Key-Regulator of Three-Dimensional Growth in Thyroid Cancer Cells Cultured under Real and Simulated Microgravity Conditions.

    Science.gov (United States)

    Riwaldt, Stefan; Bauer, Johann; Pietsch, Jessica; Braun, Markus; Segerer, Jürgen; Schwarzwälder, Achim; Corydon, Thomas J; Infanger, Manfred; Grimm, Daniela

    2015-01-01

    We recently demonstrated that the CAV1 gene was down-regulated, when poorly differentiated thyroid FTC-133 cancer cells formed spheroids under simulated microgravity conditions. Here, we present evidence that the caveolin-1 protein is involved in the inhibition of spheroid formation, when confluent monolayers are exposed to microgravity. The evidence is based on proteins detected in cells and their supernatants of the recent spaceflight experiment: "NanoRacks-CellBox-Thyroid Cancer". The culture supernatant had been collected in a special container adjacent to the flight hardware incubation chamber and stored at low temperature until it was analyzed by Multi-Analyte Profiling (MAP) technology, while the cells remaining in the incubation chamber were fixed by RNAlater and examined by mass spectrometry. The soluble proteins identified by MAP were investigated in regard to their mutual interactions and their influence on proteins, which were associated with the cells secreting the soluble proteins and had been identified in a preceding study. A Pathway Studio v.11 analysis of the soluble and cell-associated proteins together with protein kinase C alpha (PRKCA) suggests that caveolin-1 is involved, when plasminogen enriched in the extracellular space is not activated and the vascular cellular adhesion molecule (VCAM-1) mediated cell-cell adhesion is simultaneously strengthened and activated PRKCA is recruited in caveolae, while the thyroid cancer cells do not form spheroids. PMID:26633361

  6. The Importance of Caveolin-1 as Key-Regulator of Three-Dimensional Growth in Thyroid Cancer Cells Cultured under Real and Simulated Microgravity Conditions

    Directory of Open Access Journals (Sweden)

    Stefan Riwaldt

    2015-11-01

    Full Text Available We recently demonstrated that the CAV1 gene was down-regulated, when poorly differentiated thyroid FTC-133 cancer cells formed spheroids under simulated microgravity conditions. Here, we present evidence that the caveolin-1 protein is involved in the inhibition of spheroid formation, when confluent monolayers are exposed to microgravity. The evidence is based on proteins detected in cells and their supernatants of the recent spaceflight experiment: “NanoRacks-CellBox-Thyroid Cancer”. The culture supernatant had been collected in a special container adjacent to the flight hardware incubation chamber and stored at low temperature until it was analyzed by Multi-Analyte Profiling (MAP technology, while the cells remaining in the incubation chamber were fixed by RNAlater and examined by mass spectrometry. The soluble proteins identified by MAP were investigated in regard to their mutual interactions and their influence on proteins, which were associated with the cells secreting the soluble proteins and had been identified in a preceding study. A Pathway Studio v.11 analysis of the soluble and cell-associated proteins together with protein kinase C alpha (PRKCA suggests that caveolin-1 is involved, when plasminogen enriched in the extracellular space is not activated and the vascular cellular adhesion molecule (VCAM-1 mediated cell–cell adhesion is simultaneously strengthened and activated PRKCA is recruited in caveolae, while the thyroid cancer cells do not form spheroids.

  7. Enhanced Pharmacokinetics of Factor VIIa as a Monomeric Fc Fusion.

    Science.gov (United States)

    Salas, Joe; Liu, Tongyao; Lu, Qi; Kulman, John D; Ashworth, Tamera; Kistanova, Elena; Moore, Nancy; Pierce, Glenn F; Jiang, Haiyan; Peters, Robert

    2015-05-01

    Recombinant Factor VIIa (rFVIIa) is utilized for on-demand treatment of bleeding episodes in hemophilia patients with neutralizing antibodies (inhibitors) against Factor VIII or Factor IX, but a short half-life in the circulation (~2.5hrs) limits its use in a prophylactic setting. Recombinant FVIIa variants with improved pharmacokinetic properties may enable improved treatment and prevention of bleeding episodes in the inhibitor population. In this study we describe recombinant FVIIaFc (rFVIIaFc), a recombinant Fc-fusion protein generated to utilize the neonatal Fc receptor (FcRn)-mediated recycling pathway that protects immunoglobulin G from catabolism. On the basis of activity, rFVIIaFc exhibited a 5.5-fold extension in terminal half-life in hemophilia A mice compared to rFVIIa. The potency of rFVIIaFc was comparable to that of rFVIIa in thrombin generation assay and ROTEM. In agreement with these data, rFVIIaFc and rFVIIa showed similar acute efficacy at comparable molar doses in the tail clip bleeding model in hemophilia A mice. Taken together, these studies demonstrate enhanced pharmacokinetics and similar hemostatic properties for rFVIIaFc compared to rFVIIa. PMID:25721936

  8. Enhancement of Data Analysis Through Multisensor Data Fusion Technology

    Science.gov (United States)

    Multi-sensor data fusion is an emerging technology that fuses data from multiple sensors in order to make a more accurate estimation of the environment through measurement and detection. Applications of multi-sensor data fusion cross a wide spectrum in military and civilian areas. With the rapid e...

  9. Fusion

    CERN Document Server

    Mahaffey, James A

    2012-01-01

    As energy problems of the world grow, work toward fusion power continues at a greater pace than ever before. The topic of fusion is one that is often met with the most recognition and interest in the nuclear power arena. Written in clear and jargon-free prose, Fusion explores the big bang of creation to the blackout death of worn-out stars. A brief history of fusion research, beginning with the first tentative theories in the early 20th century, is also discussed, as well as the race for fusion power. This brand-new, full-color resource examines the various programs currently being funded or p

  10. Is the inhibition/enhancement of fusion due to breakup still a puzzle?

    International Nuclear Information System (INIS)

    The effect of breakup in the fusion cross section in terms of suppression versus enhancement, discussed in a conflicting way in the literature, is addressed. Data and theoretical predictions available in the literature are compared. Excitation functions of the sub- and near-barrier fusion cross-sections for a wide variety of light and heavy systems are presented and interpreted. We have measured fusion excitation functions and breakup correlation functions for the medium weight systems 6 Li + 59 Co and 7 Li + 59 Co. These measurements help to establish the influence of the projectile breakup on the fusion process at near-barrier energies and contribute to the determination of how the mass of the target affects the breakup role. The results indicate a light fusion enhancement at sub-barrier energies and a geometry dominated cross section at barrier energies. (author)

  11. A compensatory mutation provides resistance to disparate HIV fusion inhibitor peptides and enhances membrane fusion.

    Directory of Open Access Journals (Sweden)

    Matthew P Wood

    Full Text Available Fusion inhibitors are a class of antiretroviral drugs used to prevent entry of HIV into host cells. Many of the fusion inhibitors being developed, including the drug enfuvirtide, are peptides designed to competitively inhibit the viral fusion protein gp41. With the emergence of drug resistance, there is an increased need for effective and unique alternatives within this class of antivirals. One such alternative is a class of cyclic, cationic, antimicrobial peptides known as θ-defensins, which are produced by many non-human primates and exhibit broad-spectrum antiviral and antibacterial activity. Currently, the θ-defensin analog RC-101 is being developed as a microbicide due to its specific antiviral activity, lack of toxicity to cells and tissues, and safety in animals. Understanding potential RC-101 resistance, and how resistance to other fusion inhibitors affects RC-101 susceptibility, is critical for future development. In previous studies, we identified a mutant, R5-tropic virus that had evolved partial resistance to RC-101 during in vitro selection. Here, we report that a secondary mutation in gp41 was found to restore replicative fitness, membrane fusion, and the rate of viral entry, which were compromised by an initial mutation providing partial RC-101 resistance. Interestingly, we show that RC-101 is effective against two enfuvirtide-resistant mutants, demonstrating the clinical importance of RC-101 as a unique fusion inhibitor. These findings both expand our understanding of HIV drug-resistance to diverse peptide fusion inhibitors and emphasize the significance of compensatory gp41 mutations.

  12. Epithelial membrane protein 2 regulates sphingosylphosphorylcholine-induced keratin 8 phosphorylation and reorganization: Changes of PP2A expression by interaction with alpha4 and caveolin-1 in lung cancer cells.

    Science.gov (United States)

    Lee, Eun Ji; Park, Mi Kyung; Kim, Hyun Ji; Kim, Eun Ji; Kang, Gyeoung-Jin; Byun, Hyun Jung; Lee, Chang Hoon

    2016-06-01

    Sphingosylphosphorylcholine (SPC) is found at increased in the malignant ascites of tumor patients and induces perinuclear reorganization of keratin 8 (K8) filaments that contribute to the viscoelasticity of metastatic cancer cells. However, the detailed mechanism of SPC-induced K8 phosphorylation and reorganization is not clear. We observed that SPC dose-dependently reduced the expression of epithelial membrane protein 2 (EMP2) in lung cancer cells. Then, we examined the role of EMP2 in SPC-induced phosphorylation and reorganization of K8 in lung cancer cells. We found that SPC concentration-dependently reduced EMP2 in A549, H1299, and other lung cancer cells. This was verified at the mRNA level by RT-PCR and real-time PCR (qPCR), and intracellular variation through confocal microscopy. EMP2 gene silencing and stable lung cancer cell lines established using EMP2 lentiviral shRNA induced K8 phosphorylation and reorganization. EMP2 overexpression reduced K8 phosphorylation and reorganization. We also observed that SPC-induced loss of EMP2 induces phosphorylation of JNK and ERK via reduced expression of protein phosphatase 2A (PP2A). Loss of EMP2 induces ubiquitination of protein phosphatase 2A (PP2A). SPC induced caveolin-1 (cav-1) expression and EEA1 endosome marker protein but not cav-2. SPC treatment enhanced the binding of cav-1 and PP2A and lowered binding of PP2A and alpha4. Gene silencing of EMP2 increased and gene silencing of cav-1 reduced migration of A549 lung cancer cells. Overall, these results suggest that SPC induces EMP2 down-regulation which reduces the PP2A via ubiquitination induced by cav-1, which sequestered alpha4, leading to the activation of ERK and JNK. PMID:26876307

  13. Hyper-enhanced production of foreign recombinant protein by fusion with the partial polyhedrin of nucleopolyhedrovirus.

    Directory of Open Access Journals (Sweden)

    Sung Min Bae

    Full Text Available To enhance the production efficiency of foreign protein in baculovirus expression systems, the effects of polyhedrin fragments were investigated by fusion expressing them with the enhanced green fluorescent protein (EGFP. Recombinant viruses were generated to express EGFP fused with polyhedrin fragments based on the previously reported minimal region for self-assembly and the KRKK nuclear localization signal (NLS. Fusion expressions with polyhedrin amino acids 19 to 110 and 32 to 110 lead to localization of recombinant protein into the nucleus and mediate its assembly. The marked increase of EGFP by these fusion expressions was confirmed through protein and fluorescence intensity analyses. The importance of nuclear localization for enhanced production was shown by the mutation of the NLS within the fused polyhedrin fragment. In addition, when the polyhedrin fragment fused with EGFP was not localized in the nucleus, some fragments increased the production of protein. Among these fragments, some degradation of only the fused polyhedrin was observed in the fusion of amino acids 19 to 85 and 32 to 85. The fusion of amino acids 32 to 85 may be more useful for the enhanced and intact production of recombinant protein. The production of E2 protein, which is a major antigen of classical swine fever virus, was dramatically increased by fusion expression with polyhedrin amino acids 19 to 110, and its preliminary immunogenicity was verified using experimental guinea pigs. This study suggests a new option for higher expression of useful foreign recombinant protein by using the partial polyhedrin in baculovirus.

  14. Enhanced cell-free protein expression by fusion with immunoglobulin Cκ domain

    OpenAIRE

    Palmer, Elizabeth; Liu, Hong; Khan, Farid; Taussig, Michael J; He, Mingyue

    2006-01-01

    While cell-free systems are increasingly used for protein expression in structural and functional studies, several proteins are difficult to express or expressed only at low levels in cell-free lysates. Here, we report that fusion of the human immunoglobulin κ light chain constant domain (Cκ) at the C terminus of four representative proteins dramatically improved their production in the Escherichia coli S30 system, suggesting that enhancement of cell-free protein expression by Cκ fusion will ...

  15. Activation of macrophages by lymphokines: enhancement of phagosome-lysosome fusion and killing of Coccidioides immitis.

    OpenAIRE

    Beaman, L; Benjamini, E; Pappagianis, D

    1983-01-01

    Previously, it was shown that arthroconidia of Coccidioides immitis appear to inhibit phagosome-lysosome fusion and survive within normal mouse peritoneal macrophages. However, when these macrophages are exposed to antigen-stimulated T lymphocytes from immune mice, activation occurs, leading to enhanced phagosome-lysosome fusion and killing of C. immitis. Results indicate that the activation of macrophages can be effected after incubation with soluble lymphocyte product(s) (lymphokines). The ...

  16. Enhancement of fusion at near and sub-barrier energies for neutron-rich light nuclei

    CERN Document Server

    Singh, Varinderjit; Steinbach, T K; Wiggins, B B; Hudan, S; Lin, R T deSouza Zidu; Horowitz, C J; Baby, L T; Kuvin, S A; Tripathi, Vandana; Wiedenhover, I

    2016-01-01

    Measurement of the fusion cross-section for neutron-rich light nuclei is crucial in ascertaining if fusion of these nuclei occurs in the outer crust of a neutron star. We have therefore measured the fusion excitation function at near-barrier energies for the 19O + 12C system and compared the experimental results with the fusion excitation function of 18O + 12C and 16O + 12C. In the experiment a beam of 19O, produced via the 18O(d,p) reaction, was incident on a 12C target at energies near the Coulomb barrier. Evaporation residues produced in fusion of 18,19O ions with 12C target nuclei were detected with good geometric efficiency and identified by measuring their energy and time-of-flight. A significant enhancement in the fusion probability of 19O ions with a 12C target as compared to 18O ions is observed. The significantly larger cross-sections observed at near barrier energies are not predicted by a static model of fusion for 19O + 12C indicating that dynamics play an important role in the fusion of neutron-...

  17. Application of Fusion Gyrotrons to Enhanced Geothermal Systems (EGS)

    Science.gov (United States)

    Woskov, P.; Einstein, H.; Oglesby, K.

    2013-10-01

    The potential size of geothermal energy resources is second only to fusion energy. Advances are needed in drilling technology and heat reservoir formation to realize this potential. Millimeter-wave (MMW) gyrotrons and related technologies developed for fusion energy research could contribute to enabling EGS. Directed MMW energy can be used to advance rock penetration capabilities, borehole casing, and fracking. MMWs are ideally suited because they can penetrate through small particulate extraction plumes, can be efficiently guided long distances in borehole dimensions, and continuous megawatt sources are commercially available. Laboratory experiments with a 10 kW, 28 GHz CPI gyrotron have shown that granite rock can be fractured and melted with power intensities of about 1 kW/cm2 and minute exposure times. Observed melted rock MMW emissivity and estimated thermodynamics suggest that penetrating hot, hard crystalline rock formations may be economic with fusion research developed MMW sources. Supported by USDOE, Office of Energy Efficiency and Renewable Energy and Impact Technologies, LLC.

  18. Role of G(i/o)-Src kinase-PI3K/Akt pathway and caveolin-1 in β₂-adrenoceptor coupling to endothelial NO synthase in mouse pulmonary artery.

    Science.gov (United States)

    Banquet, Sébastien; Delannoy, Estelle; Agouni, Abdelali; Dessy, Chantal; Lacomme, Sabrina; Hubert, Fabien; Richard, Vincent; Muller, Bernard; Leblais, Véronique

    2011-07-01

    Activation of the β₂-adrenoceptor (β₂-AR) elicits an endothelial nitric oxide synthase (eNOS)-dependent relaxation in mouse pulmonary artery, which, contrary to the muscarinic receptor-dependent relaxation, is preserved in hypoxic pulmonary arterial hypertension. We therefore characterized the signaling pathways underlying the β₂-AR-mediated eNOS activation, with special focus on G(i/o) proteins, protein kinases and caveolae. Functional studies (for evaluation of vasorelaxant response), Western blotting (for assessment of eNOS and caveolin-1 phosphorylation) and transmission electron microscopy (for visualization of caveolae) were conducted in pulmonary arteries from wild-type or caveolin-1 knockout mice. In wild-type isolated arteries, relaxation to the selective β₂-AR agonist procaterol was reduced by inhibitors of G(i/o) proteins (pertussis toxin, PTX), phosphatidylinositol 3-kinase (PI3K; wortmannin or LY 294002), Akt (Akt inhibitor X) and Src-kinase (PP2) and by cholesterol depletion (using methyl-β-cyclodextrin). Procaterol induced eNOS phosphorylation at Ser(1177), which was prevented by PTX, PP2 or Akt inhibitor. Procaterol also promoted caveolin-1 phosphorylation at Tyr(14), which was decreased by PTX or PP2. Caveolin-1 gene deletion resulted in endothelial caveolae disruption in mouse pulmonary artery and in potentiation of procaterol-induced relaxation. Unlike procaterol, acetylcholine-induced relaxation was unaffected by PTX, methyl-β-cyclodextrin or caveolin-1 gene deletion. To conclude, the mouse pulmonary endothelial β₂-AR is coupled to a G(i/o)-Src kinase-PI3K/Akt pathway to promote eNOS phosphorylation at Ser(1177) leading to a NO-dependent vasorelaxation. Caveolin-1 exerts a negative control on this response that is abrogated by its phosphorylation at Tyr(14), through a G(i/o)-Src kinase pathway. Since pulmonary β₂-AR- and muscarinic receptor-mediated relaxations differentiate in their respective signaling pathways leading to e

  19. Reactivation coefficient of muon-catalyzed deuterium-tritium fusion and its enhancement

    Energy Technology Data Exchange (ETDEWEB)

    Takahaski, H.

    1986-03-01

    The reactivation process of a muon that is stuck to an alpha-particle produced in muon-catalyzed deuterium-tritium (D-T) fusion is studied for the different isotope targets P, D, and T by using the born approximation calculation of charge-transfer cross sections. The isotope dependence is small compared with the large isotope effects observed by Jones et al. The authors calculated density dependence is very similar to that of Bracci and Fiorentini, and it is not as large as that observed by Jones et al. The enhancement of muon reactivation by application of a high-intensity electric field to the target is studied. Even when the very high electrical field of 40 mv/cm is applied to a liquid hydrogen target, the enhancement is small because of the isotropic emission of an alpha particle in unpolarized D-T fusion. Even in polarized D-T fusion, the enhancement is small.

  20. Functional annotation of native enhancers with a Cas9 -histone demethylase fusion

    OpenAIRE

    Kearns, Nicola A; Pham, Hannah; Tabak, Barbara; Genga, Ryan M; Silverstein, Noah J; Garber, Manuel; Maehr, René

    2015-01-01

    Understanding of mammalian enhancer function is limited by the lack of a technology to rapidly and thoroughly test their cell type-specific function. Here, we use a nuclease-deficient (d)Cas9 histone demethylase fusion to functionally characterize previously described and novel enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.

  1. Functional annotation of native enhancers with a Cas9-histone demethylase fusion.

    Science.gov (United States)

    Kearns, Nicola A; Pham, Hannah; Tabak, Barbara; Genga, Ryan M; Silverstein, Noah J; Garber, Manuel; Maehr, René

    2015-05-01

    Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type-specific function. Here, we use a nuclease-deficient Cas9 (dCas9)-histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors. PMID:25775043

  2. ENHANCEMENT OF THE FUSION OF INCOMPATIBLE LISTS OF RESULTS

    Directory of Open Access Journals (Sweden)

    Guezouli Larbi

    2016-04-01

    Full Text Available This work is located in the domain of distributed information retrieval (DIR. A simplified view of the DIR requires a multi-search in a set of collections, which forces the system to analyze results found in these collections, and merge results back before sending them to the user in a single list. Our work is to find a fusion method based on the relevance score of each result received from collections and the relevance of the local search engine of each collection, which is the main issue of our work.

  3. Enhancement display of veins distribution based on binocular vision and image fusion technology

    Science.gov (United States)

    Liu, Peng; Di, Si; Jin, Jian; Bai, Liping

    2014-11-01

    The capture and display of veins distribution is an important issue for some applications, such as medical diagnosis and identification. Therefore, it has become a popular topic in the field of biomedical imaging. Usually, people capture the veins distribution by infrared imaging, but the display result is similar with that of a gray picture and the color and details of skin cannot be remained. To some degree, it is unreal for doctors. In this paper, we develop a binocular vision system to carry out the enhancement display of veins under the condition of keeping actual skin color. The binocular system is consisted of two adjacent cameras. A visible band filter and an infrared band filter are placed in front of the two lenses, respectively. Therefore, the pictures of visible band and infrared band can be captured simultaneously. After that, a new fusion process is applied to the two pictures, which related to histogram mapping, principal component analysis (PCA) and modified bilateral filter fusion. The final results show that both the veins distribution and the actual skin color of the back of the hand can be clearly displayed. Besides, correlation coefficient, average gradient and average distortion are selected as the parameters to evaluate the image quality. By comparing the parameters, it is evident that our novel fusion method is prior to some popular fusion methods such as Gauss filter fusion, Intensity-hue-saturation (HIS) fusion and bilateral filter fusion.

  4. Exercise-Induced Changes in Caveolin-1, Depletion of Mitochondrial Cholesterol, and the Inhibition of Mitochondrial Swelling in Rat Skeletal Muscle but Not in the Liver

    Directory of Open Access Journals (Sweden)

    Damian Jozef Flis

    2016-01-01

    Full Text Available The reduction in cholesterol in mitochondria, observed after exercise, is related to the inhibition of mitochondrial swelling. Caveolin-1 (Cav-1 plays an essential role in the regulation of cellular cholesterol metabolism and is required by various signalling pathways. Therefore, the aim of this study was to investigate the effect of prolonged swimming on the mitochondrial Cav-1 concentration; additionally, we identified the results of these changes as they relate to the induction of changes in the mitochondrial swelling and cholesterol in rat skeletal muscle and liver. Male Wistar rats were divided into a sedentary control group and an exercise group. The exercised rats swam for 3 hours and were burdened with an additional 3% of their body weight. After the cessation of exercise, their quadriceps femoris muscles and livers were immediately removed for experimentation. The exercise protocol caused an increase in the Cav-1 concentration in crude muscle mitochondria; this was related to a reduction in the cholesterol level and an inhibition of mitochondrial swelling. There were no changes in rat livers, with the exception of increased markers of oxidative stress in mitochondria. These data indicate the possible role of Cav-1 in the adaptive change in the rat muscle mitochondria following exercise.

  5. Enhanced Singular Value Decomposition based Fusion for Super Resolution Image Reconstruction

    Directory of Open Access Journals (Sweden)

    K. Joseph Abraham Sundar

    2015-11-01

    Full Text Available The singular value decomposition (SVD plays a very important role in the field of image processing for applications such as feature extraction, image compression, etc. The main objective is to enhance the resolution of the image based on Singular Value Decomposition. The original image and the subsequent sub-pixel shifted image, subjected to image registration is transferred to SVD domain. An enhanced method of choosing the singular values from the SVD domain images to reconstruct a high resolution image using fusion techniques is proposesed. This technique is called as enhanced SVD based fusion. Significant improvement in the performance is observed by applying enhanced SVD method preceding the various interpolation methods which are incorporated. The technique has high advantage and computationally fast which is most needed for satellite imaging, high definition television broadcasting, medical imaging diagnosis, military surveillance, remote sensing etc.

  6. Audio-Visual Based Multi-Sample Fusion to Enhance Correlation Filters Speaker Verification System

    Directory of Open Access Journals (Sweden)

    Dzati Athiar Ramli

    2010-07-01

    Full Text Available In this study, we propose a novel approach for speaker verification system that uses a spectrogram image as features and Unconstrained Minimum Average Correlation Energy (UMACE filters as classifiers. Since speech signal is a behavioral signal, the speech data has a tendency not to consistently reproduce due to the change of speaking rates, health, emotional conditions, temperature and humidity. In order to overcome this problem, a modification of UMACE filters architecture is proposed by executing a multi-sample fusion using speech and lipreading data. So as to evaluate the outstanding fusion scheme, five multisample fusion strategies, i.e. maximum, minimum, median, average and majority vote are first experimented using thespeech signal data. Afterward, the performance of the audiovisualsystem using the enhanced UMACE filters is then tested. Here, lipreading data is combined to the audio samples pool and the outstanding fusion scheme that found in prior experiment is used as multi-sample fusion scheme. The Digit Database had been used for performance evaluation and the performance up to 99.64% is achieved by using the enhanced UMACE filters for the speech only system which is 6.89% improvement compared with the base line approach. Subsequently, the implementation of the audio-visual system is observed to be significant in order to broaden the PSR score interval between the authentic and imposter data as well as to further improve the performance of audio only system that offer toward a robust verification system.

  7. A Framework for Satellite Image Enhancement Using Quantum Genetic and Weighted IHS+Wavelet Fusion Method

    Directory of Open Access Journals (Sweden)

    Amal A. HAMED

    2016-04-01

    Full Text Available this paper examined the applicability of quantum genetic algorithms to solve optimization problems posed by satellite image enhancement techniques, particularly super-resolution, and fusion. We introduce a framework starting from reconstructing the higher-resolution panchromatic image by using the subpixel-shifts between a set of lower-resolution images (registration, then interpolation, restoration, till using the higher-resolution image in pan-sharpening a multispectral image by weighted IHS+Wavelet fusion technique. For successful super-resolution, accurate image registration should be achieved by optimal estimation of subpixel-shifts. Optimal-parameters blind restoration and interpolation should be performed for the optimal quality higher-resolution image. There is a trade-off between spatial and spectral enhancement in image fusion; it is difficult for the existing methods to do the best in both aspects. The objective here is to achieve all combined requirements with optimal fusion weights, and use the parameters constraints to direct the optimization process. QGA is used to estimate the optimal parameters needed for each mathematic model in this framework “Super-resolution and fusion.” The simulation results show that the QGA-based method can be used successfully to estimate automatically the approaching parameters which need the maximal accuracy, and achieve higher quality and efficient convergence rate more than the corresponding conventional GA-based and the classic computational methods.

  8. An Approach to Optimize the Fusion Coefficients for Land Cover Information Enhancement with Multisensor Data

    Science.gov (United States)

    Garg, Akanksha; Brodu, Nicolas; Yahia, Hussein; Singh, Dharmendra

    2016-04-01

    This paper explores a novel data fusion method with the application of Machine Learning approach for optimal weighted fusion of multisensor data. It will help to get the maximum information of any land cover. Considerable amount of research work has been carried out on multisensor data fusion but getting an optimal fusion for enhancement of land cover information using random weights is still ambiguous. Therefore, there is a need of such land cover monitoring system which can provide the maximum information of the land cover, generally which is not possible with the help of single sensor data. There is a necessity to develop such techniques by which information of multisensor data can be utilized optimally. Machine learning is one of the best way to optimize this type of information. So, in this paper, the weights of each sensor data have been critically analyzed which is required for the fusion, and observed that weights are quite sensitive in fusion. Therefore, different combinations of weights have been tested exhaustively in the direction to develop a relationship between weights and classification accuracy of the fused data. This relationship can be optimized through machine learning techniques like SVM (Support Vector Machine). In the present study, this experiment has been carried out for PALSAR (Phased Array L-Band Synthetic Aperture RADAR) and MODIS (Moderate Resolution Imaging Spectroradiometer) data. PALSAR is a fully polarimetric data with HH, HV and VV polarizations at good spatial resolution (25m), and NDVI (Normalized Difference Vegetation Index) is a good indicator of vegetation, utilizing different bands (Red and NIR) of freely available MODIS data at 250m resolution. First of all, resolution of NDVI has been enhanced from 250m to 25m (10 times) using modified DWT (Modified Discrete Wavelet Transform) to bring it on the same scale as that of PALSAR. Now, different polarized PALSAR data (HH, HV, VV) have been fused with resolution enhanced NDVI

  9. Image Fusion with Contrast Enhancement Using DWT & SVD

    Directory of Open Access Journals (Sweden)

    ASHISHGOUD PURUSHOTHAM (M.Tech.

    2015-05-01

    Full Text Available Contrast is one of the important quality factors in image processing. Contrast is created by the difference in luminance reflected from two adjacent surfaces. In visual perception, contrast is determined by the difference in the colour and brightness of an object with other objects. If the contrast of an image is highly concentrated on a specific range, the information may be lost in those areas which are excessively and uniformly concentrated. This paper described a new contrast enhancement approach based on Discrete Wavelet Transform (DWT and Singular Value Decomposition (SVD that results in quality improvement of low contrast images. The input image is decomposed into four sub bands using DWT and obtain singular value matrix of the low-low sub band image and finally reconstructs the enhanced image by applying IDWT.

  10. ICRF enhancement of fusion reactivity in the presence of alpha particles

    International Nuclear Information System (INIS)

    Absorption of ICRF (ion cyclotron range of frequency) waves by alpha particles and fusion reactivity enhancement due to the ICRF induced ion tail are investigated. The rate of linear absorption by alpha particles increases with the cyclotron harmonic number, and decreases with the ratio of the electron plasma frequency to the electron cyclotron frequency. The deformation of the distribution due to ICRF waves is also examined by using a solution to a Fokker-Planck equation combined with a quasi-linear RF (radiofrequency) diffusion term. It is found that second harmonic ICRF heating is comparatively applicable to the enhancement of the fusion power density even in the presence of alpha particles, while the efficiency of the enhancement is deteriorated markedly by wave deposition to alphas for higher harmonic ICRF heating in the high magnetic field. (author)

  11. The Biometric Algorithm based on Fusion of DWT Frequency Components of Enhanced Iris Image

    Directory of Open Access Journals (Sweden)

    Rangaswamy Y

    2016-04-01

    Full Text Available The biometrics are used to authenticate a person effectively compared to conventional methods of identification. In this paper we propose the biometric algorithm based on fusion of Discrete Wavelet Transform(DWT frequency components of enhanced iris image.The iris template is extracted from an eye image by considering horizontal pixels in an iris part.The iris template contrast is enhanced using Adaptive Histogram Equalization (AHE and Histogram Equalization (HE.The DWT is applied on enhanced iris template.The features are formed by straight line fusion of low and high frequency coefficients of DWT.The Euclidian distance is used to compare final test features with database features. It is observed that the performance parameters are better in the case of proposed algorithm compared to existing algorithms.

  12. Helium-3 induced enhancement of tritium production for fusion reactors

    International Nuclear Information System (INIS)

    This report provides the results of an inquiry into the feasibility of enhancing tritium production levels through the activation of helium-3 following its external addition to the moderator system of a CANDU reactor. The physical basis for the scheme lies in the fact that the cross section for the activation of helium-3 to tritium is several orders of magnitude larger than the cross section for deuterium activation. The imminent introduction of a centralized facility for the removal, immobilization, and storage of tritium ensures a supply of helium-3, the product of tritium decay

  13. Enhanced bactericidal potency of nanoliposomes by modification of the fusion activity between liposomes and bacterium

    Directory of Open Access Journals (Sweden)

    Ma YF

    2013-06-01

    amount of negative charges in fluid liposomes reduces fluid liposomes-bacteria fusion when tested without calcium cations due to electric repulsion, but addition of calcium cations brings the fusion level of fluid liposomes to similar or higher levels. Among the negative phospholipids examined, DMPA gave the highest degree of fusion, DMPS and DMPG had intermediate fusion levels, and PI resulted in the lowest degree of fusion. Furthermore, the fluid liposomal encapsulated tobramycin was prepared, and the bactericidal effect occurred more quickly when bacteria were cultured with liposomal encapsulated tobramycin. Conclusion: The bactericidal potency of fluid liposomes is dramatically enhanced with respect to fusion ability when the fusogenic lipid, DOPE, is included. Regardless of changes in liposome composition, fluid liposomes-bacterium fusion is universally enhanced by calcium ions. The information obtained in this study will increase our understanding of fluid liposomal action mechanisms, and help in optimizing the new generation of fluid liposomal formulations for the treatment of pulmonary bacterial infections. Keywords: liposomes, fusion, bacteria, Pseudomonas aeruginosa, lipid composition

  14. Relationship with spatial memory in diabetic rats and protein kinase Cγ,caveolin-1 in the hippocampus and neuroprotective effect of catalpol

    Institute of Scientific and Technical Information of China (English)

    Zhou Haicheng; Liu Jing; Ren Liyuan; Liu Wei; Xing Qian; Men Lili; Song Guirong

    2014-01-01

    Background The mechanisms underlying diabetic encephalopathy are largely unknown,and no effective treatments are available.Catalpol has received much attention due to its numerous biological effects,especially in neuroprotective studies.The aim of this study was to investigate the effects of catalpol on cognitive functions in diabetic rats and the underlying mechanisms.Methods A rat model of diabetes was established by streptozotocin injection,followed by intraperitoneal infusion of catalpol after 10 weeks.Two weeks later,the Morris water maze was used to test the spatial learning performance.Nissl staining was performed to evaluate the morphological changes in the hippocampus.Expression of protein kinase Cy (PKCy) and caveolin-1 (Cav-1) in the hippocampus were assessed by reverse transcription PCR and Western blotting.Activities of anti-oxidative enzymes such as glutathione (GSH),superoxide dismutase (SOD) and catalase (CAT) and levels of malonaldehyde (MDA) were measured using commercial kits.Results Significant hippocampal neuronal injury was observed in rats with streptozotocin-induced diabetes.Moreover,cognitive dysfunction was associated with markedly increased oxidative stress in the brain.Catalpol treatment significantly attenuated cognitive deficits,neuronal damage,and oxidative stress in the brain of diabetic rats.Biochemical analyses showed that catalpol reversed the down-regulation of PKCγ and Cav-1 expression in the diabetic rats.Conclusions Spatial memory in diabetic rats is associated with the expression of PKCy and Cav-1.Catalpol treatment markedly attenuated oxidative stress,reversed the alteration of PKCy,Cav-1 and spatial memory deficits.

  15. Integrin α1β1 Regulates Epidermal Growth Factor Receptor Activation by Controlling Peroxisome Proliferator-Activated Receptor γ-Dependent Caveolin-1 Expression ▿ # ‖

    Science.gov (United States)

    Chen, Xiwu; Whiting, Carrie; Borza, Corina; Hu, Wen; Mont, Stacey; Bulus, Nada; Zhang, Ming-Zhi; Harris, Raymond C.; Zent, Roy; Pozzi, Ambra

    2010-01-01

    Integrin α1β1 negatively regulates the generation of profibrotic reactive oxygen species (ROS) by inhibiting epidermal growth factor receptor (EGFR) activation; however, the mechanism by which it does this is unknown. In this study, we show that caveolin-1 (Cav-1), a scaffolding protein that binds integrins and controls growth factor receptor signaling, participates in integrin α1β1-mediated EGFR activation. Integrin α1-null mesangial cells (MCs) have reduced Cav-1 levels, and reexpression of the integrin α1 subunit increases Cav-1 levels, decreases EGFR activation, and reduces ROS production. Downregulation of Cav-1 in wild-type MCs increases EGFR phosphorylation and ROS synthesis, while overexpression of Cav-1 in the integrin α1-null MCs decreases EGFR-mediated ROS production. We further show that integrin α1-null MCs have increased levels of activated extracellular signal-regulated kinase (ERK), which leads to reduced activation of peroxisome proliferator-activated receptor γ (PPARγ), a transcription factor that positively regulates Cav-1 expression. Moreover, activation of PPARγ or inhibition of ERK increases Cav-1 levels in the integrin α1-null MCs. Finally, we show that glomeruli of integrin α1-null mice have reduced levels of Cav-1 and activated PPARγ but increased levels of phosphorylated EGFR both at baseline and following injury. Thus, integrin α1β1 negatively regulates EGFR activation by positively controlling Cav-1 levels, and the ERK/PPARγ axis plays a key role in regulating integrin α1β1-dependent Cav-1 expression and consequent EGFR-mediated ROS production. PMID:20368353

  16. Integrin alpha1beta1 regulates epidermal growth factor receptor activation by controlling peroxisome proliferator-activated receptor gamma-dependent caveolin-1 expression.

    Science.gov (United States)

    Chen, Xiwu; Whiting, Carrie; Borza, Corina; Hu, Wen; Mont, Stacey; Bulus, Nada; Zhang, Ming-Zhi; Harris, Raymond C; Zent, Roy; Pozzi, Ambra

    2010-06-01

    Integrin alpha1beta1 negatively regulates the generation of profibrotic reactive oxygen species (ROS) by inhibiting epidermal growth factor receptor (EGFR) activation; however, the mechanism by which it does this is unknown. In this study, we show that caveolin-1 (Cav-1), a scaffolding protein that binds integrins and controls growth factor receptor signaling, participates in integrin alpha1beta1-mediated EGFR activation. Integrin alpha1-null mesangial cells (MCs) have reduced Cav-1 levels, and reexpression of the integrin alpha1 subunit increases Cav-1 levels, decreases EGFR activation, and reduces ROS production. Downregulation of Cav-1 in wild-type MCs increases EGFR phosphorylation and ROS synthesis, while overexpression of Cav-1 in the integrin alpha1-null MCs decreases EGFR-mediated ROS production. We further show that integrin alpha1-null MCs have increased levels of activated extracellular signal-regulated kinase (ERK), which leads to reduced activation of peroxisome proliferator-activated receptor gamma (PPARgamma), a transcription factor that positively regulates Cav-1 expression. Moreover, activation of PPARgamma or inhibition of ERK increases Cav-1 levels in the integrin alpha1-null MCs. Finally, we show that glomeruli of integrin alpha1-null mice have reduced levels of Cav-1 and activated PPARgamma but increased levels of phosphorylated EGFR both at baseline and following injury. Thus, integrin alpha1beta1 negatively regulates EGFR activation by positively controlling Cav-1 levels, and the ERK/PPARgamma axis plays a key role in regulating integrin alpha1beta1-dependent Cav-1 expression and consequent EGFR-mediated ROS production. PMID:20368353

  17. Sex-dependent expression of caveolin 1 in response to sex steroid hormones is closely associated with development of obesity in rats.

    Directory of Open Access Journals (Sweden)

    Rajib Mukherjee

    Full Text Available Caveolin-1 (CAV1 is a conserved group of structural membrane proteins that form special cholesterol and sphingolipid-rich compartments, especially in adipocytes. Recently, it has been reported that CAV1 is an important target protein in sex hormone-dependent regulation of various metabolic pathways, particularly in cancer and diabetes. To clarify distinct roles of CAV1 in sex-dependent obesity development, we investigated the effects of high fat diet (HFD and sex steroid hormones on CAV1 expression in adipose tissues of male and female rats. Results of animal experiments revealed that estrogen (17-β-estradiol, E2 and androgen (dihydrotestosterone, DHT had opposite effects on body weight gain as well as on the regulation of CAV1, hormone sensitive lipase (HSL and uncoupling protein 1 (UCP1 in adipose tissues. Furthermore, sex hormone receptors and aromatase were differentially expressed in a sex-dependent manner in response to E2 and DHT treatments. In vivo data were confirmed using 3T3-L1 and HIB1B cell lines, where Cav1 knock down stimulated lipogenesis but suppressed sex hormone receptor signaling proteins. Most importantly, co-immunoprecipitation enabled the identification of previously unrecognized CAV1-interacting mitochondrial or lipid oxidative pathway proteins in adipose tissues. Taken together, current data showed that CAV1 may play important preventive role in the development of obesity, with more prominent effects in females, and proved to be an important target protein for the hormonal regulation of adipose tissue metabolism by manipulating sex hormone receptors and mitochondrial oxidative pathways. Therefore, we can report, for the first time, the molecular mechanism underlying the effects of sex steroid hormones in the sex-dimorphic regulation of CAV1.

  18. Cross-talk between Dopachrome Tautomerase and Caveolin-1 Is Melanoma Cell Phenotype-specific and Potentially Involved in Tumor Progression.

    Science.gov (United States)

    Popa, Ioana L; Milac, Adina L; Sima, Livia E; Alexandru, Petruta R; Pastrama, Florin; Munteanu, Cristian V A; Negroiu, Gabriela

    2016-06-10

    l-Dopachrome tautomerase (l-DCT), also called tyrosinase-related protein-2 (TRP-2), is a melanoma antigen overexpressed in most chemo-/radiotherapeutic stress-resistant tumor clones, and caveolin-1 (CAV1) is a main regulator of numerous signaling processes. A structural and functional relationship between DCT and CAV1 is first presented here in two human amelanotic melanoma cell lines, derived from vertical growth phase (MelJuSo) and metastatic (SKMel28) melanomas. DCT co-localizes at the plasma membrane with CAV1 and Cavin-1, another molecular marker for caveolae in both cell phenotypes. Our novel structural model proposed for the DCT-CAV1 complex, in addition to co-immunoprecipitation and mass spectrometry data, indicates a possible direct interaction between DCT and CAV1. The CAV1 control on DCT gene expression, DCT post-translational processing, and subcellular distribution is cell phenotype-dependent. DCT is a modulator of CAV1 stability and supramolecular assembly in both cell phenotypes. During autocrine stimulation, the expressions of DCT and CAV1 are oppositely regulated; DCT increases while CAV1 decreases. Sub-confluent MelJuSo clones DCT(high)/CAV1(low) are proliferating and acquire fibroblast-like morphology, forming massive, confluent clusters as demonstrated by immunofluorescent staining and TissueFAXS quantitative image cytometry analysis. CAV1 down-regulation directly contributes to the expansion of MelJuSo DCT(high) subtype. CAV1 involved in the perpetuation of cell phenotype-overexpressing anti-stress DCT molecule supports the concept that CAV1 functions as a tumor suppressor in early stages of melanoma. DCT is a regulator of the CAV1-associated structures and is possibly a new molecular player in CAV1-mediated processes in melanoma. PMID:27053106

  19. Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer

    Science.gov (United States)

    He, Yuyu; Zhao, Xianda; Gao, Jun; Fan, Lifang; Yang, Guifang; Cho, William Chi-shing; Chen, Honglei

    2012-01-01

    Caveolin-1 (Cav-1) expression deficiency and autophagy in tumor stromal fibroblasts (hereafter fibroblasts) are involved in tumor proliferation and progression, particularly in breast and prostate cancer. The aim of this study was to detect the expression of fibroblastic Cav-1 and LC3B, markers of autophagy, in gastric cancer (GC) and to analyze their clinical significances. Furthermore, because Epstein-Barr virus (EBV)-associated GC (EBVaGC) is a unique subtype of GC; we compared the differential expression of fibroblastic Cav-1 and LC3B in EBVaGC and non-EBVaGC. Quantum dots (QDs)-based immunofluorescence histochemistry was used to examine the expression of fibroblastic Cav-1 and LC3B in 118 cases of GC with adequate stroma. QDs-based double immunofluorescence labeling was performed to detect the coexpression of Cav-1 and LC3B proteins. EBV-encoded small RNA was detected by QDs-based fluorescence in situ hybridization to identify EBVaGC. Multivariate analysis indicated that low fibroblastic Cav-1 level was an independent prognosticator (p = 0.029) that predicted poorer survival of GC patients. Positive fibroblastic LC3B was correlated with lower invasion (p = 0.032) and was positively associated with Cav-1 expression (r = 0.432, p < 0.001). EBV infection did not affect fibroblastic Cav-1 and LC3B expression. In conclusion, positive fibroblastic LC3B correlates with lower invasion, and low expression of fibroblastic Cav-1 is a novel predictor of poor GC prognosis. PMID:23203033

  20. ITER and IAEA to enhance co-operation on fusion research

    International Nuclear Information System (INIS)

    Full text: The IAEA and the International Thermonuclear Experimental Reactor (ITER) Organization today signed an agreement that will enhance the research of fusion, a form of nuclear energy created by the merging of light atoms. The Co-operation Agreement is aimed at strengthening the working relationship between both organizations 'with a view to facilitating the effective attainment of the objectives set forth in the IAEA Statute and the ITER Agreement.' Yury Sokolov, Deputy Director General of the International Atomic Energy Agency (IAEA), and Director General of the ITER Organization, Kaname Ikeda, signed the agreement on the opening day of the 22nd IAEA Fusion Energy Conference, being held in Geneva, Switzerland, from 13 to 18 October. The IAEA has been closely involved with ITER since its inception, as the previous ITER co-operation phases and the ITER negotiations were held under its auspices. The IAEA Director-General is also the Depository of the ITER Agreement. 'The IAEA has played a crucial role in the evolution of the ITER project,' said Mr. Ikeda. 'We look forward to continued, dynamic collaboration.' Mr. Sokolov added that the IAEA has helped the international fusion community to focus its plasma physics research on the ITER design. This has made fusion research more results-oriented and has encouraged greater global investment in fusion. 'ITER is a clear example of how a large international project can be successfully organized. We are proud of the IAEA's role in ITER's development,' he said. According to the Co-operation Agreement, both organizations will exchange information regarding the study and potential application of fusion energy and will participate in each other's meeting. These will include ITER Council meetings and Annual Conferences of the IAEA, as well as its scientific and technical committees. The IAEA and the ITER Organization will also co-operate on training, publications, organization of scientific conferences, plasma physics and

  1. Enhancing accuracy of magnetic resonance image fusion by defining a volume of interest

    International Nuclear Information System (INIS)

    We compared the registration accuracy for corresponding anatomical landmarks in two MR images after fusing the complete volume (CV) and a defined volume of interest (VOI) of both MRI data sets. We carried out contrast-enhanced T1-weighted gradient-echo and T2-weighted fast spin-echo MRI (matrix 256 x 256) in 39 cases. The CV and a defined VOI data set were each fused using prototype software. We measured and analysed the distance between 25 anatomical landmarks in predefined areas identified at levels L1-L5 corresponding to defined axial sections. Fusion technique, landmark areas and level of fusion were further processed using a feed-forward neural network to calculate the difference which can be expected based on the measurements. We identified 975 landmarks for both T1- and T2-weighted images and found a significant difference in registration accuracy (P1) to caudal (L5), mean deviations were: L1 CV 1.5 mm, VOI 0.5 mm; L2 CV 1.8 mm, VOI 0.4 mm; L3 CV 1.7 mm, VOI 0.4 mm; L4 CV 1.6 mm, VOI 0.6 mm; and L5 CV 1.6 mm, VOI 1.6 mm. Neural network analysis predicted a higher accuracy for VOI (0.05-0.15 mm) than for CV fusion (0.9-1.6 mm). Deviations due to magnetic susceptibility changes between air and tissue seen on gradient-echo images can decrease fusion accuracy. Our VOI fusion technique improves image fusion accuracy to <0.5 mm by excluding areas with marked susceptibility changes. (orig.)

  2. Design and characterization of novel recombinant listeriolysin O-protamine fusion proteins for enhanced gene delivery.

    Science.gov (United States)

    Kim, Na Hyung; Provoda, Chester; Lee, Kyung-Dall

    2015-02-01

    To improve the efficiency of gene delivery for effective gene therapy, it is essential that the vector carries functional components that can promote overcoming barriers in various steps leading to the transport of DNA from extracellular to ultimately nuclear compartment. In this study, we designed genetically engineered fusion proteins as a platform to incorporate multiple functionalities in one chimeric protein. Prototypes of such a chimera tested here contain two domains: one that binds to DNA; the other that can facilitate endosomal escape of DNA. The fusion proteins are composed of listeriolysin O (LLO), the endosomolytic pore-forming protein from Listeria monocytogenes, and a 22 amino acid sequence of the DNA-condensing polypeptide protamine (PN), singly or as a pair: LLO-PN and LLO-PNPN. We demonstrate dramatic enhancement of the gene delivery efficiency of protamine-condensed DNA upon incorporation of a small amount of LLO-PN fusion protein and further improvement with LLO-PNPN in vitro using cultured cells. Additionally, the association of anionic liposomes with cationic LLO-PNPN/protamine/DNA complexes, yielding a net negative surface charge, resulted in better in vitro transfection efficiency in the presence of serum. An initial, small set of data in mice indicated that the observed enhancement in gene expression could also be applicable to in vivo gene delivery. This study suggests that incorporation of a recombinant fusion protein with multiple functional components, such as LLO-protamine fusion protein, in a nonviral vector is a promising strategy for various nonviral gene delivery systems. PMID:25521817

  3. Enhanced SUMOylation of proteins containing a SUMO-interacting motif by SUMO-Ubc9 fusion

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Eui Tae; Kim, Kyeong Kyu [Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 300 Cheoncheondong Jangangu, Suwon, Gyeonggido 440-746 (Korea, Republic of); Matunis, Mike J. [Department of Biochemistry and Molecular Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD (United States); Ahn, Jin-Hyun, E-mail: jahn@med.skku.ac.kr [Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 300 Cheoncheondong Jangangu, Suwon, Gyeonggido 440-746 (Korea, Republic of)

    2009-10-09

    Identifying new targets for SUMO and understanding the function of protein SUMOylation are largely limited by low level of SUMOylation. It was found recently that Ubc9, the SUMO E2 conjugating enzyme, is covalently modified by SUMO at a lysine 14 in the N-terminal alpha helix, and that SUMO-modified Ubc9 has enhanced conjugation activity for certain target proteins containing a SUMO-interacting motif (SIM). Here, we show that, compared to intact Ubc9, the SUMO-Ubc9 fusion protein has higher conjugating activity for SIM-containing targets such as Sp100 and human cytomegalovirus IE2. Assays using an IE2 SIM mutant revealed the requirement of SIM for the enhanced IE2 SUMOylation by SUMO-Ubc9. In pull-down assays with cell extracts, the SUMO-Ubc9 fusion protein bound to more diverse cellular proteins and interacted with some SIM-containing proteins with higher affinities than Ubc9. Therefore, the devised SUMO-Ubc9 fusion will be useful for identifying SIM-containing SUMO targets and producing SUMO-modified proteins.

  4. Volume navigation with contrast enhanced ultrasound and image fusion for percutaneous interventions: first results.

    Directory of Open Access Journals (Sweden)

    Ernst Michael Jung

    Full Text Available OBJECTIVE: Assessing the feasibility and efficiency of interventions using ultrasound (US volume navigation (V Nav with real time needle tracking and image fusion with contrast enhanced (ce CT, MRI or US. METHODS: First an in vitro study on a liver phantom with CT data image fusion was performed, involving the puncture of a 10 mm lesion in a depth of 5 cm performed by 15 examiners with US guided freehand technique vs. V Nav for the purpose of time optimization. Then 23 patients underwent ultrasound-navigated biopsies or interventions using V Nav image fusion of live ultrasound with ceCT, ceMRI or CEUS, which were acquired before the intervention. A CEUS data set was acquired in all patients. Image fusion was established for CEUS and CT or CEUS and MRI using anatomical landmarks in the area of the targeted lesion. The definition of a virtual biopsy line with navigational axes targeting the lesion was achieved by the usage of sterile trocar with a magnetic sensor embedded in its distal tip employing a dedicated navigation software for real time needle tracking. RESULTS: The in vitro study showed significantly less time needed for the simulated interventions in all examiners when V Nav was used (p<0.05. In the study involving patients, in all 10 biopsies of suspect lesions of the liver a histological confirmation was achieved. We also used V Nav for a breast biopsy (intraductal carcinoma, for a biopsy of the abdominal wall (metastasis of ovarial carcinoma and for radiofrequency ablations (4 ablations. In 8 cases of inflammatory abdominal lesions 9 percutaneous drainages were successfully inserted. CONCLUSION: Percutaneous biopsies and drainages, even of small lesions involving complex access pathways, can be accomplished with a high success rate by using 3D real time image fusion together with real time needle tracking.

  5. Packed fluidization, enhancement of heat transfer in pebble bed and thermonuclear fusion technology

    International Nuclear Information System (INIS)

    Packed fluidization is a technique in which small particles (size: 100-800 μm) are allowed to fluidize in the interstices of stationary pebbles (size: >1.0 mm). Packed fluidization enhances the rate of heat transfer in pebble bed at low operative gas velocity as well as at low pressure drop across the bed. Experiments were conducted to study heat transfer in unary packed bed and binary packed fluidized bed using lithium titanate and alumina pebbles (size: 3-10 mm) and lithium titanate and silica particles (size: 231-780 μm). It was found that due to packed fluidization the rate of heat transfer is enhanced and arms of the effective thermal conductivity this enhancement was up to 260%. Low thermal conductivity of pebble bed of solid breeder materials is one of the adverse key issues which must be addressed properly for the successful development of the thermonuclear fusion technology. Packed fluidization enhances the effective thermal conductivity of the pebble bed of solid breeder materials in the Test Blanket Module (TBM) of ITER type fusion reactor. (author)

  6. Fusion of Synthetic and Enhanced Vision for All-Weather Commercial Aviation Operations

    Science.gov (United States)

    Bailey, Randall E.; Kramer, Lynda J.; Prinzel, Lawrence, III

    2007-01-01

    NASA is developing revolutionary crew-vehicle interface technologies that strive to proactively overcome aircraft safety barriers that would otherwise constrain the full realization of the next-generation air transportation system. A piloted simulation experiment was conducted to evaluate the complementary use of Synthetic and Enhanced Vision technologies. Specific focus was placed on new techniques for integration and/or fusion of Enhanced and Synthetic Vision and its impact within a two-crew flight deck during low visibility approach and landing operations. Overall, the experimental data showed that significant improvements in situation awareness, without concomitant increases in workload and display clutter, could be provided by the integration and/or fusion of synthetic and enhanced vision technologies for the pilot-flying and the pilot-not-flying. During non-normal operations, the ability of the crew to handle substantial navigational errors and runway incursions were not adversely impacted by the display concepts although the addition of Enhanced Vision did not, unto itself, provide an improvement in runway incursion detection.

  7. Nuclear Fusion Within Extremely Dense Plasma Enhanced by Quantum Particle Waves

    Science.gov (United States)

    Miao, Feng; Zheng, Xianjun; Deng, Baiquan

    2015-05-01

    Quantum effects play an enhancement role in p-p chain reactions occurring within stars. Such an enhancement is quantified by a wave penetration factor that is proportional to the density of the participating fuel particles. This leads to an innovative theory for dense plasma, and its result shows good agreement with independent data derived from the solar energy output. An analysis of the first Z-pinch machine in mankind's history exhibiting neutron emission leads to a derived deuterium plasma beam density greater than that of water, with plasma velocities exceeding 10000 km/s. Fusion power could be achieved by the intersection of four such pinched plasma beams with powerful head-on collisions in their common focal region due to the beam and target enhanced reaction. supported by the Fund for the Construction of Graduate Degree of China (No. 2014XWD-S0805)

  8. Quantitative Characterization of Inertial Confinement Fusion Capsules Using Phase Contrast Enhanced X-Ray Imaging

    International Nuclear Information System (INIS)

    Current designs for inertial confinement fusion capsules for the National Ignition Facility (NIF) consist of a solid deuterium-tritium (D-T) fuel layer inside of a copper doped beryllium capsule. Phase contrast enhanced x-ray imaging is shown to render the D-T layer visible inside the Be(Cu) capsule. Phase contrast imaging is experimentally demonstrated for several surrogate capsules and validates computational models. Polyimide and low density divinyl benzene foam capsules were imaged at the Advanced Photon Source synchrotron. The surrogates demonstrate that phase contrast enhanced imaging provides a method to characterize surfaces when absorption imaging cannot be used. Our computational models demonstrate that a rough surface can be accurately reproduced in phase contrast enhanced x-ray images

  9. Quantitative characterization of inertial confinement fusion capsules using phase contrast enhanced x-ray imaging

    International Nuclear Information System (INIS)

    Current designs for inertial confinement fusion capsules for the National Ignition Facility consist of a solid deuterium-tritium (D-T) fuel layer inside of a copper doped beryllium, Be(Cu), shell. Phase contrast enhanced x-ray imaging is shown to render the D-T layer visible inside the Be(Cu) shell. Phase contrast imaging is experimentally demonstrated for several surrogate capsules and validates computational models. Polyimide and low density divinyl benzene foam shells were imaged at the Advanced Photon Source synchrotron. The surrogates demonstrate that phase contrast enhanced imaging provides a method to characterize surfaces when absorption imaging cannot be used. Our computational models demonstrate that a rough surface can be accurately characterized using phase contrast enhanced x-ray images

  10. Enhanced charged Higgs production through W -Higgs fusion in W - b scattering

    Science.gov (United States)

    Arhrib, Abdesslam; Cheung, Kingman; Lee, Jae Sik; Lu, Chih-Ting

    2016-05-01

    We study the associated production of a charged Higgs boson with a bottom quark and a light quark at the LHC via pp → H ± b j in the Two Higgs Doublet Models (2HDMs). Using the effective W approximation, we show that there is exact cancellation among various Feynman diagrams in high energy limit. This may imply that the production of charged Higgs can be significantly enhanced in the presence of large mass differences among the neutral Higgs bosons via W ±-Higgs fusion in the pp → H ± b j process. Particularly, we emphasize the potential enhancement due to a light pseudoscalar boson A, which is still allowed by the current data by which we explicitly calculate the allowed regions in ( M A , tan β) plane, and show that the production cross section can be as large as 0.1 pb for large tan β. We also show that the transverse momentum distribution of the b quark can potentially distinguish the W ± - A fusion diagram from the top diagram. Finally, we point out further enhancement when we go beyond the 2HDMs.

  11. Fast Ignition Thermonuclear Fusion: Enhancement of the Pellet Gain by the Colossal-Magnetic-Field Shells

    Science.gov (United States)

    Stefan, V. Alexander

    2013-10-01

    The fast ignition fusion pellet gain can be enhanced by a laser generated B-field shell. The B-field shell, (similar to Earth's B-field, but with the alternating B-poles), follows the pellet compression in a frozen-in B-field regime. A properly designed laser-pellet coupling can lead to the generation of a B-field shell, (up to 100 MG), which inhibits electron thermal transport and confines the alpha-particles. In principle, a pellet gain of few-100s can be achieved in this manner. Supported in part by Nikola Tesla Labs, Stefan University, 1010 Pearl, La Jolla, CA 92038-1007.

  12. Enhanced immunogenicity of DNA fusion vaccine encoding secreted hepatitis B surface antigen and chemokine RANTES

    International Nuclear Information System (INIS)

    To increase the potency of DNA vaccines, we constructed genetic fusion vaccines encoding antigen, secretion signal, and/or chemokine RANTES. The DNA vaccines encoding secreted hepatitis B surface antigen (HBsAg) were constructed by inserting HBsAg gene into an expression vector with an endoplasmic reticulum (ER)-targeting secretory signal sequence. The plasmid encoding secretory HBsAg (pER/HBs) was fused to cDNA of RANTES, generating pER/HBs/R. For comparison, HBsAg genes were cloned into pVAX1 vector with no signal sequence (pHBs), and further linked to the N-terminus of RANTES (pHBs/R). Immunofluorescence study showed the cytoplasmic localization of HBsAg protein expressed from pHBs and pHBs/R, but not from pER/HBs and pER/HBs/R at 48 h after transfection. In mice, RANTES-fused DNA vaccines more effectively elicited the levels of HBsAg-specific IgG antibodies than pHBs. All the DNA vaccines induced higher levels of IgG2a rather than IgG1 antibodies. Of RANTES-fused vaccines, pER/HBs/R encoding the secreted fusion protein revealed much higher humoral and CD8+ T cell-stimulating responses compared to pHBs/R. These results suggest that the immunogenicity of DNA vaccines could be enhanced by genetic fusion to a secretory signal peptide sequence and RANTES

  13. Changes of caveolin-1 expression in the hippocampus of rats after diffuse axonal injury and its relationship with activation of astroglia%大鼠弥漫性轴索损伤后海马区caveolin-1的表达及其与星形胶质细胞活化的关系

    Institute of Scientific and Technical Information of China (English)

    赵永林; 宋锦宁; 马旭东; 张斌飞; 张明; 李丹东; 庞宏刚; 罗显华

    2014-01-01

    目的 研究大鼠弥漫性轴索损伤(diffuse axonal injury,DAD后小窝蛋白1(caveolin-1)在大鼠脑海马区的表达,探讨caveolin-1的表达变化与星形胶质细胞活化的关系.方法 运用Western blot检测大鼠海马中caveolin-1的表达,运用免疫组化检测海马区胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的表达,并通过免疫荧光共定位分析caveolin-1与GFAP之间的相互作用.结果 Western blot结果提示DAI后海马区caveolin-1的表达从1d开始显著降低,并持续降低至7 d(P<0.05);免疫组化结果提示DAI后星形胶质细胞活化标志物GFAP的表达从1d后逐渐升高,3d后达峰,7d后稍降低,但仍高于正常(P<0.05);免疫荧光共定位提示DAI后caveolin-1与GFAP的共定位细胞数随着时间的延长逐渐增加.结论 大鼠DAI后海马区caveolin-1的表达减少,GFAP的表达增加,星形胶质细胞内caveolin-1的表达变化可能是导致GFAP升高及星形胶质细胞活化的机制之一.

  14. Subbarrier heavy ion fusion enhanced by nucleon transfer and subbarrier fusion of nuclei far from the line of $\\beta$-stability

    CERN Document Server

    Denisov, V Yu

    1998-01-01

    We discuss a model for the description of subbarrier fusion of heavy ions which takes into account the coupling to the low-energy surface vibrational states and to the few-nucleon transfer with arbitrary reaction Q-value. The fusion reactions ^{28,30}Si+^{58,62,64}Ni, ^{40}Ca+^{90,96}Zr, ^{28}S+^{94,100}Mo, ^{16,18,20,22,24}O+^{58}Ni and ^{28}Si+^{124,126,128,130,132}Sn are analyzed in detail. The model describes rather well the experimental fusion cross section and mean angular momentum for reactions between nuclei near the \\beta-stability line. It is shown that these quantities are significantly enhanced by few-nucleon transfer with large positive Q-value. A shape independent parameterization of the heavy ion potential at distances smaller then the touching point is proposed.

  15. Deuterium-Tritium Simulations of the Enhanced Reversed Shear Mode in the Tokamak Fusion Test Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Mikkelsen, D.R.; Manickam, J.; Scott, S.D.; Zarnstorff

    1997-04-01

    The potential performance, in deuterium-tritium plasmas, of a new enhanced con nement regime with reversed magnetic shear (ERS mode) is assessed. The equilibrium conditions for an ERS mode plasma are estimated by solving the plasma transport equations using the thermal and particle dif- fusivities measured in a short duration ERS mode discharge in the Tokamak Fusion Test Reactor [F. M. Levinton, et al., Phys. Rev. Letters, 75, 4417, (1995)]. The plasma performance depends strongly on Zeff and neutral beam penetration to the core. The steady state projections typically have a central electron density of {approx}2:5x10 20 m{sup -3} and nearly equal central electron and ion temperatures of {approx}10 keV. In time dependent simulations the peak fusion power, {approx} 25 MW, is twice the steady state level. Peak performance occurs during the density rise when the central ion temperature is close to the optimal value of {approx} 15 keV. The simulated pressure profiles can be stable to ideal MHD instabilities with toroidal mode number n = 1, 2, 3, 4 and {infinity} for {beta}{sub norm} up to 2.5; the simulations have {beta}{sub norm} {le} 2.1. The enhanced reversed shear mode may thus provide an opportunity to conduct alpha physics experiments in conditions imilar to those proposed for advanced tokamak reactors.

  16. Enhanced HIV-1 neutralization by a CD4-VH3-IgG1 fusion protein

    International Nuclear Information System (INIS)

    HIV-1 gp120 is an alleged B cell superantigen, binding certain VH3+ human antibodies. We reasoned that a CD4-VH3 fusion protein could possess higher affinity for gp120 and improved HIV-1 inhibitory capacity. To test this we produced several human IgG1 immunoligands harboring VH3. Unlike VH3-IgG1 or VH3-CD4-IgG1, CD4-VH3-IgG1 bound gp120 considerably stronger than CD4-IgG1. CD4-VH3-IgG1 exhibited ∼1.5-2.5-fold increase in neutralization of two T-cell laboratory-adapted strains when compared to CD4-IgG1. CD4-VH3-IgG1 improved neutralization of 7/10 clade B primary isolates or pseudoviruses, exceeding 20-fold for JR-FL and 13-fold for Ba-L. It enhanced neutralization of 4/8 clade C viruses, and had negligible effect on 1/4 clade A pseudoviruses. We attribute this improvement to possible pairing of VH3 with CD4 D1 and stabilization of an Ig Fv-like structure, rather than to superantigen interactions. These novel findings support the current notion that CD4 fusion proteins can act as better HIV-1 entry inhibitors with potential clinical implications.

  17. Enhanced Computational Infrastructure for Data Analysis at the DIII-D National Fusion Facility

    International Nuclear Information System (INIS)

    Recently a number of enhancements to the computer hardware infrastructure have been implemented at the DIII-D National Fusion Facility. Utilizing these improvements to the hardware infrastructure, software enhancements are focusing on streamlined analysis, automation, and graphical user interface (GUI) systems to enlarge the user base. The adoption of the load balancing software package LSF Suite by Platform Computing has dramatically increased the availability of CPU cycles and the efficiency of their use. Streamlined analysis has been aided by the adoption of the MDSplus system to provide a unified interface to analyzed DIII-D data. The majority of MDSplus data is made available in between pulses giving the researcher critical information before setting up the next pulse. Work on data viewing and analysis tools focuses on efficient GUI design with object-oriented programming (OOP) for maximum code flexibility. Work to enhance the computational infrastructure at DIII-D has included a significant effort to aid the remote collaborator since the DIII-D National Team consists of scientists from 9 national laboratories, 19 foreign laboratories, 16 universities, and 5 industrial partnerships. As a result of this work, DIII-D data is available on a 24 x 7 basis from a set of viewing and analysis tools that can be run either on the collaborators' or DIII-Ds computer systems. Additionally, a Web based data and code documentation system has been created to aid the novice and expert user alike

  18. Enhanced computational infrastructure for data analysis at the DIII-D National Fusion Facility

    International Nuclear Information System (INIS)

    Recently a number of enhancements to the computer hardware infrastructure have been implemented at the DIII-D National Fusion Facility. Utilizing these improvements to the hardware infrastructure, software enhancements are focusing on streamlined analysis, automation, and graphical user interface (GUI) systems to enlarge the user base. The adoption of the load balancing software package LSF Suite by Platform Computing has dramatically increased the availability of CPU cycles and the efficiency of their use. Streamlined analysis has been aided by the adoption of the MDSplus system to provide a unified interface to analyzed DIII-D data. The majority of MDSplus data is made available in between pulses giving the researcher critical information before setting up the next pulse. Work on data viewing and analysis tools focuses on efficient GUI design with object-oriented programming (OOP) for maximum code flexibility. Work to enhance the computational infrastructure at DIII-D has included a significant effort to aid the remote collaborator since the DIII-D National Team consists of scientists from nine national laboratories, 19 foreign laboratories, 16 universities, and five industrial partnerships. As a result of this work, DIII-D data is available on a 24x7 basis from a set of viewing and analysis tools that can be run on either the collaborators' or DIII-D's computer systems. Additionally, a web based data and code documentation system has been created to aid the novice and expert user alike

  19. Oligomerization of Fusogenic Peptides Promotes Membrane Fusion by Enhancing Membrane Destabilization

    OpenAIRE

    Lau, Wai Leung; Ege, David S.; Lear, James D.; Hammer, Daniel A.; DeGrado, William F.

    2004-01-01

    A key element of membrane fusion reactions in biology is the involvement of specific fusion proteins. In many viruses, the proteins that mediate membrane fusion usually exist as homotrimers. Furthermore, they contain extended triple-helical coiled-coil domains and fusogenic peptides. It has been suggested that the coiled-coil domains present the fusogenic peptide in a conformation or geometry favorable for membrane fusion. To test the hypothesis that trimerization of fusogenic peptide is rela...

  20. Demineralized Bone Matrix, as a Graft Enhancer of Auto-Local Bone in Posterior Lumbar Interbody Fusion

    OpenAIRE

    Ahn, Dong Ki; Moon, Sang Ho; Kim, Tae Woo; Boo, Kyung Hwan; Hong, Sung Won

    2014-01-01

    Study Design A case controlled study with prospective data collection. Purpose To evaluate the early influence and the final consequence of demineralized bone matrix (DBM) on auto-local bone as a graft enhancer in posterior lumbar interbody fusion (PLIF). Overview of Literature DBM is known as an osteoinductive material; however, it has not been clearly recognized to enhance auto-local bone with a small amount. Methods Patients who had a PLIF were allocated into two groups. Group I (70 cases)...

  1. Enhancement of monacolin K production via intergeneric protoplast fusion between Aspergillus terreus and Monascus anka

    Institute of Scientific and Technical Information of China (English)

    Chen Zhi; Lin Wen; Yu Ping; Song Yuan

    2007-01-01

    Intergenric protoplast fusion between Aspergillus terreus CA99 and Monascus anka M-3, the high and low producers of monacolin K respectively, was performed for enhancement of monacolin K production. The 24-hour-old mycelia of A. terreus CA99 and M. anka M-3 were treated with 0.5 % lywallzyme, 0.3 % snailase and 0.3 % cellulase at 34 ℃ for 5 h and at 30 ℃ for 3.5 h, and their protoplasts formation reached 1.76 × 107/mL and 1.68 × 107/mL respectively. Parental protoplasts were irradiated with a 30 W UVlight away from 30 cm for 3 min and then mixed. The mixture was incubated with 30% PEG 6000 for 15 min. The reviving fusants were isolated on the regeneration plates. Of the 363 fusants isolated, over 100 showed enhanced monacolin K production compared with the parental strain M. anka M-3. Ten of them produced monacolin K about 1.6-fold of that M. anka M-3 does and the monacolin K titer of two fusants (F49 and F104) increased by about 1-fold. The monacolin K yields of F49 and F104 were 460 μg/mL and 457 μg/mL respectively. In optimized fermentation medium, the monacolin K titer of F49 reached 1216 μg/mL.

  2. Enhanced efficacy of CTLA-4 fusion anti-caries DNA vaccines in gnotobiotic hamsters

    Institute of Scientific and Technical Information of China (English)

    Feng ZHANG; Yu-hong LI; Ming-wen FAN; Rong JIA; Qing-an XU; Ji-hua GUO; Fei YU; Qi-wei TIAN

    2007-01-01

    Aim:To evaluate the comparative immunogenicity and protective efficacy of the cytotoxic T-lymphocyte.associated antigen 4(CTLA-4)fusion anti-caries DNA vaccines pGJA-P/VAX1,pGJA-P,and non-fusion anti-caries DNA construct pGLUA-P in hamsters.In addition,the ability of CTLA-4 to target pGJA-P/VAX1-encoding antigen to dendritic cells was tested in vitro.Methods:All DNA constructs contain genes encoding the A-P regions of a cell surface protein(PAc) and the glucan binding(GLU) domain of glucosyltransferases(GTFs)of cari-ogenic organism Streptococcus mutans.Human dendritic cells were mixed with the CTLA-4-Ig-GLU-A-P protein expressed by pGJA-P/VAX1-transfected cells and analyzed by flow cytometry.Gnotobiotic hamsters were immunized with anti-caries DNA vaccines by intramuscular injection or intranasal administration.Antibody responses to a representative antigen PAc were assayed by ELlSA,and caries protection was evaluated by Keyes caries scores.Results:A flow cytometric analysis demonstrated that CTLA-4-Ig-GLU-A-P protein was capable of bind-ing to human dendritic cells.pGJA-P/VAX1 and pGJA-P induced significantly higher specific salivary and serum anti-PAc antibody responses than pGLUA-R.Significantly fewet caries lesions were alSO observed in hamsters immunized with pGJA-P/VAX1 and pGJA-p There was no significant difference in the anti-PAC antibody level or caries scores between pGJA-P/VAX1 and pGJA-P-immunized groups.Conclusion:Antigen encoded by CTLA-4 fusion anti-caries DNA vac-cine pGJA-P/VAX1 could specifically bind to human dendritic cells through the interaction of CTLA-4 and B7 molecules.Fusing antigen to CTLA-4 has been proven to greatly enhance the immunogenicity and protective efficacy of anti-caries DNA vaccines.

  3. Enhancement of sub-barrier fusion cross section in the weakly bound neutron system, 11Be + 10Be

    International Nuclear Information System (INIS)

    The sub-barrier fusion cross section for the weakly bound neutron system, 11Be + 10Be is discussed in the framework of the coupled-reaction-channel approach for the valence neutron in 11Be and in connection with the molecular orbital formation. In the calculation we observed a big enhancement of the fusion process, due to very strong multi-step processes in the inelastic and transfer transitions of the active neutron, which lead to the formation of a covalent molecule, 10Be + n + 10Be. (author)

  4. 糖尿病足截肢患者股神经中小窝蛋白1的表达及意义%Expression and significance of caveolin-1 in femoral nerve of diabetic foot amputation patients

    Institute of Scientific and Technical Information of China (English)

    丁敏; 褚月颉; 徐俊; 章鸣放; 赵凤云; 王鹏华

    2010-01-01

    Objective To investigate the expression and significance of caveolin-1 in femoral nerve of diabetic patients with foot amputation. Methods Forty patients with foot amputation were assigned to 3 groups according to their duration of type 2 diabetes: group A ( <6 years=, group B (6-10 years), and group C ( >10 years). Hematoxylin and eosin (HE) stain and Weil's stain were used to examine the femoral nerve. Silver staining was used to observe the axons and to count the nerve fiber density. The expression of caveolin-1 in Schwann cells of femoral nerve was tested by immunohistochemisty. Results There were evident progressive pathological changes in femoral nerve in the 3 groups. The variance of nerve fiber density in the 3 groups reached statistical significance ( P<0. 05 =, the nerve fiber density showed negative correlation with HbA1C( r =-0. 792, P<0. 01 = and duration ( r=-0.592, P<0. 01 =. The expression of caveolin-1 in Schwann cells of femoral nerve was positive in all the 3 groups and the variance with statistical significance (P<0. 01 ), it was negatively correlated with HbA1C (r=-0. 762, P<0. 01 )and duration (r=-0. 532, P<0. 01 ), and it was positively correlated with nerve fiber density (r=0. 721, P<0.01 ), the partial correlation coefficient of caveolin-1 and HbA1Cwas-0. 505 ( P<0. 01 ).Conclusion In patients with diabetic foot amputation, caveolin-1 may play a role in the development of diabetic peripheral neuropathy and diabetic foot.%目的 探讨糖尿病足截肢患者股神经雪旺氏细胞中小窝蛋白1的表达及意义.方法 40例糖尿病足截肢患者根据2型糖尿病病程分为A(<6年),B(6~10年),C(>10年)3组.HE、Weil氏染色观察股神经病理学改变,银染法进行轴突染色并计数股神经纤维密度.应用免疫组化染色检测雪旺氏细胞中小窝蛋白1的表达.结果 3组股神经均存在明显的病理改变,随着病程延长病变加重.3组间股神经纤维密

  5. Enhancement and selective production of avermectin B by recombinants of Streptomyces avermitilis via intraspecific protoplast fusion

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhi; WEN Jia; SONG Yuan; WEN Ying; LI JiLun

    2007-01-01

    Among eight components of avermectin, B1 fractions have the most effective antiparasitic activities and the lowest level of toxic side-effects and are used widely in veterinary and agricultural fields. Intraspecific protoplast fusion between two strains of Streptomyces avermitilis, one an avermectin high producer (strain 76-05) and the other a genetically engineered strain containing the mutations aveD- and olmA- (strain 73-12) was performed for enhancement and selective production of avermectin B in the absence of oligomycin. Two recombinant strains (F23 and F29) were isolated and characterized with regards to the parental merits. F23 and F29 produced only the four avermectin B components with high yield and produced no oligomycin. The avermectin production of F23 and F29 was about 84.20% and 103.45% of the parental strain 76-05, respectively, and increased about 2.66-fold and 3.50-fold, respectively, compared to that of parental strain 73-12. F23 and F29 were genetically stable prototrophic recombinants and F29 was quite tolerant of fermentation conditions compared to avermectin high producer parental strain 76-05. The ability to produce avermectin B with high yield without the production of other avermectin components and oligomycin will make F23 and F29 useful strains for avermectin production. Strain F29's tolerance of fermentation conditions will also make it suitable for industrial applications.

  6. Enhanced neutralization potency of botulinum neurotoxin antibodies using a red blood cell-targeting fusion protein.

    Directory of Open Access Journals (Sweden)

    Sharad P Adekar

    Full Text Available Botulinum neurotoxin (BoNT potently inhibits cholinergic signaling at the neuromuscular junction. The ideal countermeasures for BoNT exposure are monoclonal antibodies or BoNT antisera, which form BoNT-containing immune complexes that are rapidly cleared from the general circulation. Clearance of opsonized toxins may involve complement receptor-mediated immunoadherence to red blood cells (RBC in primates or to platelets in rodents. Methods of enhancing immunoadherence of BoNT-specific antibodies may increase their potency in vivo. We designed a novel fusion protein (FP to link biotinylated molecules to glycophorin A (GPA on the RBC surface. The FP consists of an scFv specific for murine GPA fused to streptavidin. FP:mAb:BoNT complexes bound specifically to the RBC surface in vitro. In a mouse model of BoNT neutralization, the FP increased the potency of single and double antibody combinations in BoNT neutralization. A combination of two antibodies with the FP gave complete neutralization of 5,000 LD50 BoNT in mice. Neutralization in vivo was dependent on biotinylation of both antibodies and correlated with a reduction of plasma BoNT levels. In a post-exposure model of intoxication, FP:mAb complexes gave complete protection from a lethal BoNT/A1 dose when administered within 2 hours of toxin exposure. In a pre-exposure prophylaxis model, mice were fully protected for 72 hours following administration of the FP:mAb complex. These results demonstrate that RBC-targeted immunoadherence through the FP is a potent enhancer of BoNT neutralization by antibodies in vivo.

  7. R and D for tritium removal system using bacteria for safety enhancement of tritium handling in fusion reactor

    International Nuclear Information System (INIS)

    In order to safety enhancement of fusion reactor, Japan Atomic Energy Agency (JAEA) investigates effective tritium removal system in case of incidental tritium release in the building. Recently, we paid attention to the bacteria, which had an ability of hydrogen oxidation at room temperature, and a new effective detritiation system using the bacteria has been studied under the collaboration with Ibaraki University, instead of conventional detritiation system using catalytic oxidation reactor of rare metal. (author)

  8. A new method for fusion, denoising and enhancement of x-ray images retrieved from Talbot-Lau grating interferometry

    Science.gov (United States)

    Scholkmann, Felix; Revol, Vincent; Kaufmann, Rolf; Baronowski, Heidrun; Kottler, Christian

    2014-03-01

    This paper introduces a new image denoising, fusion and enhancement framework for combining and optimal visualization of x-ray attenuation contrast (AC), differential phase contrast (DPC) and dark-field contrast (DFC) images retrieved from x-ray Talbot-Lau grating interferometry. The new image fusion framework comprises three steps: (i) denoising each input image (AC, DPC and DFC) through adaptive Wiener filtering, (ii) performing a two-step image fusion process based on the shift-invariant wavelet transform, i.e. first fusing the AC with the DPC image and then fusing the resulting image with the DFC image, and finally (iii) enhancing the fused image to obtain a final image using adaptive histogram equalization, adaptive sharpening and contrast optimization. Application examples are presented for two biological objects (a human tooth and a cherry) and the proposed method is compared to two recently published AC/DPC/DFC image processing techniques. In conclusion, the new framework for the processing of AC, DPC and DFC allows the most relevant features of all three images to be combined in one image while reducing the noise and enhancing adaptively the relevant image features. The newly developed framework may be used in technical and medical applications.

  9. A new method for fusion, denoising and enhancement of x-ray images retrieved from Talbot–Lau grating interferometry

    International Nuclear Information System (INIS)

    This paper introduces a new image denoising, fusion and enhancement framework for combining and optimal visualization of x-ray attenuation contrast (AC), differential phase contrast (DPC) and dark-field contrast (DFC) images retrieved from x-ray Talbot–Lau grating interferometry. The new image fusion framework comprises three steps: (i) denoising each input image (AC, DPC and DFC) through adaptive Wiener filtering, (ii) performing a two-step image fusion process based on the shift-invariant wavelet transform, i.e. first fusing the AC with the DPC image and then fusing the resulting image with the DFC image, and finally (iii) enhancing the fused image to obtain a final image using adaptive histogram equalization, adaptive sharpening and contrast optimization. Application examples are presented for two biological objects (a human tooth and a cherry) and the proposed method is compared to two recently published AC/DPC/DFC image processing techniques. In conclusion, the new framework for the processing of AC, DPC and DFC allows the most relevant features of all three images to be combined in one image while reducing the noise and enhancing adaptively the relevant image features. The newly developed framework may be used in technical and medical applications. (paper)

  10. Chloroquine Increases Glucose Uptake via Enhancing GLUT4 Translocation and Fusion with the Plasma Membrane in L6 Cells

    Directory of Open Access Journals (Sweden)

    Qi Zhou

    2016-05-01

    Full Text Available Background/Aims: Chloroquine can induce an increase in the cellular uptake of glucose; however, the underlying mechanism is unclear. Methods: In this study, translocation of GLUT4 and intracellular Ca2+ changes were simultaneously observed by confocal microscope in L6 cells stably over-expressing IRAP-mOrange. The GLUT4 fusion with the plasma membrane (PM was traced using HA-GLUT4-GFP. Glucose uptake was measured using a cell-based glucose uptake assay. GLUT4 protein was detected by Western blotting and mRNA level was detected by RT-PCR. Results: We found that chloroquine induced significant increases in glucose uptake, glucose transporter GLUT4 translocation to the plasma membrane (GTPM, GLUT4 fusion with the PM, and intracellular Ca2+ in L6 muscle cells. Chloroquine-induced increases of GTPM and intracellular Ca2+ were inhibited by Gallein (Gβγ inhibitor and U73122 (PLC inhibitor. However, 2-APB (IP3R blocker only blocked the increase in intracellular Ca2+ but did not inhibit GTPM increase. These results indicate that chloroquine, via the Gβγ-PLC-IP3-IP3R pathway, induces elevation of Ca2+, and this Ca2+ increase does not play a role in chloroqui-ne-evoked GTPM increase. However, GLUT4 fusion with the PM and glucose uptake were significantly inhibited with BAPTA-AM. This suggests that Ca2+ enhances GLUT4 fusion with the PM resulting in glucose uptake increase. Conclusion: Our data indicate that chloroquine via Gβγ-PLC-IP3-IP3R induces Ca2+ elevation, which in turn promotes GLUT4 fusion with the PM. Moreover, chloroquine can enhance GLUT4 trafficking to the PM. These mechanisms eventually result in glucose uptake increase in control and insulin-resistant L6 cells. These findings suggest that chloroquine might be a potential drug for improving insulin tolerance in diabetic patients.

  11. Neutron degradation of UV enhanced optical fibers for fusion installations in plasma diagnostics

    International Nuclear Information System (INIS)

    The design of ITER and the future operation of DEMO will require high-temperature and high-neutron flux resistant materials to be used in plasma diagnostics subject to ITER requirements. A solution for remote plasma diagnostics implies the operation of various optical instruments placed apart from the critical temperature-neutron zones with the optical signal transmitted over optical channels for a reduction of radiation effects on the equipment and a higher resistance to electromagnetic disturbances. Generally, data are available on the radiation effects on optical fibers used in communication applications or for transmission in the visible range. A special problem arises when optical signals have to be transmitted in the UV region (200 nm to 450 nm), where attenuation over the distance is quite high (in the range of several meters) and the UV radiation by itself produces an increase of the attenuation after several hours of exposure. Until now, practically no data on the radiation effects on optical fibers for fusion plasma diagnostics operating in UV were published. In the frame of the EU funded Fusion Programme, we focused on the evaluation of radiation induced changes in the optical transmission for different commercially available optical fibers for their possible use in optical light guides. Pure silica optical fibers with an UV enhanced response with various cladding/jacket materials and core diameter of 200 μm and 400 μm were evaluated as they are subject to neutron irradiation. The optical fiber core was of a high hydroxyl content type and the coating was either Polyimide or aluminium. Optical fibers with low (150 deg C) and high (350 deg C) temperature jacket materials were investigated. The optical fiber samples were irradiated at fast neutron facility of the IFIN-HH (Horia Hulubei National Institute for Physics and Nuclear Engineering) U-120 Cyclotron. The neutron flux at 0 angle (i.e. in the beam direction) was found to be equivalent to 2.13 x 108 n

  12. Tunnelling effect enhanced by lattice screening as main cold fusion mechanism: An brief theoretical overview

    International Nuclear Information System (INIS)

    In this paper are illustrated the main features of tunneling traveling between two deuterons within a lattice. Considering the screening effect due lattice electrons we compare the d-d fusion rate evaluated from different authors assuming different screening efficiency and different d-d potentials. Then, we propose a effective potential which describe very well the attractive contribute due to plasmon exchange between two deuterons and by means of it we will compute the d-d fusion rates for different energy values. Finally the good agreement between theoretical and experimental results proves the reality of cold fusion phenomena and the reliability of our model

  13. Fusion Physics

    International Nuclear Information System (INIS)

    Recreating the energy production process of the Sun - nuclear fusion - on Earth in a controlled fashion is one of the greatest challenges of this century. If achieved at affordable costs, energy supply security would be greatly enhanced and environmental degradation from fossil fuels greatly diminished. Fusion Physics describes the last fifty years or so of physics and research in innovative technologies to achieve controlled thermonuclear fusion for energy production. The International Atomic Energy Agency (IAEA) has been involved since its establishment in 1957 in fusion research. It has been the driving force behind the biennial conferences on Plasma Physics and Controlled Thermonuclear Fusion, today known as the Fusion Energy Conference. Hosted by several Member States, this biennial conference provides a global forum for exchange of the latest achievements in fusion research against the backdrop of the requirements for a net energy producing fusion device and, eventually, a fusion power plant. The scientific and technological knowledge compiled during this series of conferences, as well as by the IAEA Nuclear Fusion journal, is immense and will surely continue to grow in the future. It has led to the establishment of the International Thermonuclear Experimental Reactor (ITER), which represents the biggest experiment in energy production ever envisaged by humankind.

  14. Antibody-Dependent Enhancement of Dengue Virus Infection in Primary Human Macrophages; Balancing Higher Fusion against Antiviral Responses.

    Science.gov (United States)

    Flipse, Jacky; Diosa-Toro, Mayra A; Hoornweg, Tabitha E; van de Pol, Denise P I; Urcuqui-Inchima, Silvio; Smit, Jolanda M

    2016-01-01

    The dogma is that the human immune system protects us against pathogens. Yet, several viruses, like dengue virus, antagonize the hosts' antibodies to enhance their viral load and disease severity; a phenomenon called antibody-dependent enhancement of infection. This study offers novel insights in the molecular mechanism of antibody-mediated enhancement (ADE) of dengue virus infection in primary human macrophages. No differences were observed in the number of bound and internalized DENV particles following infection in the absence and presence of enhancing concentrations of antibodies. Yet, we did find an increase in membrane fusion activity during ADE of DENV infection. The higher fusion activity is coupled to a low antiviral response early in infection and subsequently a higher infection efficiency. Apparently, subtle enhancements early in the viral life cycle cascades into strong effects on infection, virus production and immune response. Importantly, and in contrast to other studies, the antibody-opsonized virus particles do not trigger immune suppression and remain sensitive to interferon. Additionally, this study gives insight in how human macrophages interact and respond to viral infections and the tight regulation thereof under various conditions of infection. PMID:27380892

  15. Antibody-Dependent Enhancement of Dengue Virus Infection in Primary Human Macrophages; Balancing Higher Fusion against Antiviral Responses

    Science.gov (United States)

    Flipse, Jacky; Diosa-Toro, Mayra A.; Hoornweg, Tabitha E.; van de Pol, Denise P. I.; Urcuqui-Inchima, Silvio; Smit, Jolanda M.

    2016-01-01

    The dogma is that the human immune system protects us against pathogens. Yet, several viruses, like dengue virus, antagonize the hosts’ antibodies to enhance their viral load and disease severity; a phenomenon called antibody-dependent enhancement of infection. This study offers novel insights in the molecular mechanism of antibody-mediated enhancement (ADE) of dengue virus infection in primary human macrophages. No differences were observed in the number of bound and internalized DENV particles following infection in the absence and presence of enhancing concentrations of antibodies. Yet, we did find an increase in membrane fusion activity during ADE of DENV infection. The higher fusion activity is coupled to a low antiviral response early in infection and subsequently a higher infection efficiency. Apparently, subtle enhancements early in the viral life cycle cascades into strong effects on infection, virus production and immune response. Importantly, and in contrast to other studies, the antibody-opsonized virus particles do not trigger immune suppression and remain sensitive to interferon. Additionally, this study gives insight in how human macrophages interact and respond to viral infections and the tight regulation thereof under various conditions of infection. PMID:27380892

  16. Refraction-Enhanced X-ray Radiography for Inertial Confinement Fusion and Laser-Produced Plasma Applications

    Energy Technology Data Exchange (ETDEWEB)

    Koch, J A; Landen, O L; Kozioziemski, B J; Izumi, N; Dewald, E L; Salmonson, J D; Hammel, B A

    2008-08-26

    We explore various laser-produced plasma and inertial-confinement fusion (ICF) applications of phase-contrast x-ray radiography, and we show how the main features of these enhancements can be considered from a geometrical optics perspective as refraction enhancements. This perspective simplifies the analysis, and often permits simple analytical formulae to be derived that predict the enhancements. We explore a raytrace approach to various material interface applications, and we explore a more general example of refractive bending of x-rays by an implosion plasma. We find that refraction-enhanced x-ray radiography of implosions may provide a means to quantify density differences across shock fronts as well as density variations caused by local heating due to high-Z dopants. We also point out that refractive bending by implosions plasmas can blur fine radiograph features, and can also provide misleading contrast information in area-backlit pinhole imaging experiments unless its effects are taken into consideration.

  17. Neisseria gonorrhoeae phagosomes delay fusion with primary granules to enhance bacterial survival inside human neutrophils.

    Science.gov (United States)

    Johnson, M Brittany; Criss, Alison K

    2013-08-01

    Symptomatic infection with Neisseria gonorrhoeae (Gc) promotes inflammation driven by polymorphonuclear leucocytes (PMNs, neutrophils), yet some Gc survive PMN exposure during infection. Here we report a novel mechanism of gonococcal resistance to PMNs: Gc phagosomes avoid maturation into phagolysosomes by delayed fusion with primary (azurophilic) granules, which contain antimicrobial components including serine proteases. Reduced phagosome-primary granule fusion was observed in gonorrheal exudates and human PMNs infected ex vivo. Delayed phagosome-granule fusion could be overcome by opsonizing Gc with immunoglobulin. Using bacterial viability dyes along with antibodies to primary granules revealed that Gc survival in PMNs correlated with early residence in primary granule-negative phagosomes. However, when Gc was killed prior to PMN exposure, dead bacteria were also found in primary granule-negative phagosomes. These results suggest that Gc surface characteristics, rather than active bacterial processes, influence phagosome maturation and that Gc death inside PMNs occurs after phagosome-granule fusion. Ectopically increasing primary granule-phagosome fusion, by immunoglobulin opsonization or PMN treatment with lysophosphatidylcholine, reduced intracellular Gc viability, which was attributed in part to serine protease activity. We conclude that one method for Gc to avoid PMN clearance in acute gonorrhoea is by delaying primary granule-phagosome fusion, thus preventing formation of a degradative phagolysosome. PMID:23374609

  18. Tumor necrosis factor-α enhanced fusions between oral squamous cell carcinoma cells and endothelial cells via VCAM-1/VLA-4 pathway

    International Nuclear Information System (INIS)

    Fusion between cancer cells and host cells, including endothelial cells, may strongly modulate the biological behavior of tumors. However, no one is sure about the driving factors and underlying mechanism involved in such fusion. We hypothesized in this study that inflammation, one of the main characteristics in tumor microenvironment, serves as a prominent catalyst for fusion events. Our results showed that oral cancer cells can fuse spontaneously with endothelial cells in co-culture and inflammatory cytokine tumor necrosis factor-α (TNF-α) increased fusion of human umbilical vein endothelium cells and oral cancer cells by up to 3-fold in vitro. Additionally, human oral squamous cell carcinoma cell lines and 35 out of 50 (70%) oral squamous carcinoma specimens express VLA-4, an integrin, previously implicated in fusions between human peripheral blood CD34-positive cells and murine cardiomyocytes. Expression of VCAM-1, a ligand for VLA-4, was evident on vascular endothelium of oral squamous cell carcinoma. Moreover, immunocytochemistry and flow cytometry analysis revealed that expression of VCAM-1 increased obviously in TNF-α-stimulated endothelial cells. Anti-VLA-4 or anti-VCAM-1 treatment can decrease significantly cancer–endothelial adhesion and block such fusion. Collectively, our results suggested that TNF-α could enhance cancer–endothelial cell adhesion and fusion through VCAM-1/VLA-4 pathway. This study provides insights into regulatory mechanism of cancer–endothelial cell fusion, and has important implications for the development of novel therapeutic strategies for prevention of metastasis. -- Highlights: ► Spontaneous oral cancer–endothelial cell fusion. ► TNF-α enhanced cell fusions. ► VCAM-1/VLA-4 expressed in oral cancer. ► TNF-α increased expression of VCAM-1 on endothelial cells. ► VCAM-1/VLA-4 mediated TNF-α-enhanced cell fusions.

  19. Lensfree Spectral Light-field Fusion Microscopy for Contrast- and Resolution-enhanced Imaging of Biological Specimens

    CERN Document Server

    Kazemzadeh, Farnoud; Molladavoodi, Sara; Mei, Yu; Emelko, Monica B; Gorbet, Maud B; Wong, Alexander

    2015-01-01

    A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing interferometric light-field encodings at different wavelengths, and Bayesian-based fusion to reconstruct a fused object light-field from the encodings. By fusing unique object detail information captured at different wavelengths, LSLFM can achieve improved resolution, contrast, and signal-to-noise ratio (SNR) over a single-channel lensfree microscopy system. A five-channel LSLFM system was developed and quantitatively evaluated to validate the design. Experimental results demonstrated that the LSLFM system provided SNR improvements of 6.81-16.55 dB, as well as a six-fold improvement in the dispersion index (DI), over that achieved using a single-channel lensfree deconvolution microscopy system at individual wavelengths. Furthermore, the LSLFM system achieved an increase in numeric...

  20. APPLICATION OF IMAGE FUSION FOR ENHANCING THE QUALITY OF AN IMAGE

    Directory of Open Access Journals (Sweden)

    Suman Deb

    2012-07-01

    Full Text Available Advances in technology have brought about extensive research in the field of image fusion. Image fusion is one of the most researched challenges of Face Recognition. Face Recognition (FR is the process by which the brain and mind understand, interpret and identify or verify human faces.. Image fusion is the combination of two or more source images which vary in resolution, instrument modality, or image capture technique into a single composite representation. Thus, the source images are complementary in many ways, with no one input image being an adequate data representation of the scene. Therefore, the goal of an image fusion algorithm is to integrate the redundant and complementary information obtained from the source images in order to form a new image which provides a better description of the scene for human or machine perception. In this paper we have proposed a novel approach of pixel level image fusion using PCA that will remove the image blurredness in two images and reconstruct a new de-blurred fused image. The proposed approach is based on the calculation of Eigen faces with Principal Component Analysis (PCA. Principal Component Analysis (PCA has been most widely used method for dimensionality reduction and feature extraction.

  1. Enhanced loss of fusion products during mode conversion heating in TFTR

    International Nuclear Information System (INIS)

    Ion Bernstein waves (IBWS) have been generated by mode conversion of ion cyclotron range of frequency (ICRF) fast waves in TFTR. The loss rate of fusion products in these discharges can be large, up to 10 times the first orbit loss rate. The losses are observed at the passing/trapped boundary, indicating that passing particles are being moved onto loss orbits either by increase of their v perpendicular due to the wave, by outward transport in minor radius, or both. The lost particles appear to be DD fusion produced tritons heated to ∼1.5 times their birth energy

  2. Directing oncogenic fusion genes into stem cells via an SCL enhancer

    OpenAIRE

    Eguchi, Mariko; Eguchi-Ishimae, Minenori; Green, Anthony; Enver, Tariq; Greaves, Mel

    2005-01-01

    TEL-TRKC is a fusion gene generated by chromosomal translocation and encodes an activated tyrosine kinase. Uniquely, it is found in both solid tumors and leukemia. However, a single exon difference (in TEL) in TEL-TRKC fusions is associated with the two sets of cancer phenotypes. We expressed the two TEL-TRKC variants in vivo by using the 3′ regulatory element of SCL that is selectively active in a subset of mesodermal cell lineages, including endothelial and hematopoietic stem cells and prog...

  3. Enhanced loss of fusion products during mode conversion heating in TFTR

    International Nuclear Information System (INIS)

    Ion Bernstein waves (IBWs) have been generated by mode conversion of ion cyclotron range of frequency (ICRF) fast waves in TFTR. The loss rate of fusion products in these discharges can be large, up to 10 times the first orbit loss rate. The losses are observed at the passing/trapped boundary, indicating that passing particles are being moved onto loss orbits either by increase of their v perpendicular due to the wave, by outward transport in minor radius, or both. The lost particles appear to be DD fusion produced tritons heated to ∼1.5 times their birth energy. copyright 1996 American Institute of Physics

  4. Isotopic dependence of fusion enhancement of various heavy ion systems using energy dependent Woods–Saxon potential

    Energy Technology Data Exchange (ETDEWEB)

    Gautam, Manjeet Singh, E-mail: gautammanjeet@gmail.com

    2015-01-15

    In the present work, the fusion of symmetric and asymmetric projectile–target combinations are deeply analyzed within the framework of energy dependent Woods–Saxon potential model (EDWSP model) in conjunction with one dimensional Wong formula and the coupled channel code CCFULL. The neutron transfer channels and the inelastic surface excitations of collision partners are dominating mode of couplings and the coupling of relative motion of colliding nuclei to such relevant internal degrees of freedom produces a significant fusion enhancement at sub-barrier energies. It is quite interesting that the effects of dominant intrinsic degrees of freedom such as multi-phonon vibrational states, neutron transfer channels and proton transfer channels can be simulated by introducing the energy dependence in the nucleus–nucleus potential (EDWSP model). In the EDWSP model calculations, a wide range of diffuseness parameter ranging from a=0.85 fm to a=0.97 fm, which is much larger than a value (a=0.65 fm) extracted from the elastic scattering data, is needed to reproduce sub-barrier fusion data. However, such diffuseness anomaly, which might be an artifact of some dynamical effects, has been resolved by trajectory fluctuation dissipation (TFD) model wherein the resulting nucleus–nucleus potential possesses normal diffuseness parameter.

  5. Isotopic dependence of fusion enhancement of various heavy ion systems using energy dependent Woods-Saxon potential

    Science.gov (United States)

    Gautam, Manjeet Singh

    2015-01-01

    In the present work, the fusion of symmetric and asymmetric projectile-target combinations are deeply analyzed within the framework of energy dependent Woods-Saxon potential model (EDWSP model) in conjunction with one dimensional Wong formula and the coupled channel code CCFULL. The neutron transfer channels and the inelastic surface excitations of collision partners are dominating mode of couplings and the coupling of relative motion of colliding nuclei to such relevant internal degrees of freedom produces a significant fusion enhancement at sub-barrier energies. It is quite interesting that the effects of dominant intrinsic degrees of freedom such as multi-phonon vibrational states, neutron transfer channels and proton transfer channels can be simulated by introducing the energy dependence in the nucleus-nucleus potential (EDWSP model). In the EDWSP model calculations, a wide range of diffuseness parameter ranging from a = 0.85 fm to a = 0.97 fm, which is much larger than a value (a = 0.65 fm) extracted from the elastic scattering data, is needed to reproduce sub-barrier fusion data. However, such diffuseness anomaly, which might be an artifact of some dynamical effects, has been resolved by trajectory fluctuation dissipation (TFD) model wherein the resulting nucleus-nucleus potential possesses normal diffuseness parameter.

  6. Recording system and data fusion algorithm for enhancing the estimation of the respiratory rate from photoplethysmogram.

    Science.gov (United States)

    Cernat, Roxana A; Ciorecan, Silvia I; Ungureanu, Constantin; Arends, Johan; Strungaru, Rodica; Ungureanu, G Mihaela

    2015-08-01

    The respiratory rate is a vital parameter that can provide valuable information about the health condition of a patient. The extraction of respiratory information from photoplethysmographic signal (PPG) was actually encouraged by the reported results, our main goal being to obtain accurate respiratory rate estimation from the PPG signal. We developed a fusion algorithm that identifies the best derived respiratory signals, from which is possible to extract the respiratory rate; based on these, a global respiratory rate is computed using the proposed fusion algorithm. The algorithm is qualitatively tested on real PPG signals recorded by an acquisition system we implemented, using a reflection pulse oximeter sensor. Its performance is also statistically evaluated using benchmark dataset publically available from CapnoBase.Org. PMID:26737653

  7. Enhanced ocean temperature forecast skills through 3-D super-ensemble multi-model fusion

    OpenAIRE

    F. Lenartz; Mourre, B.; Barth, A.; Beckers, J.-M.; Vandenbulcke, L.; M. Rixen

    2010-01-01

    An innovative multi-model fusion technique is proposed to improve short-term ocean temperature forecasts: the three-dimensional super-ensemble. In this method, a Kalman Filter is used to adjust three-dimensional model weights over a past learning period, allowing to give more importance to recent observations, and take into account spatially varying model skills. The predictive performance is evaluated against SST analyses, CTD casts and gliders tracks collected during the Ligurian Sea Cal/Va...

  8. Enhancing image fusion performance and utility for defence and security applications

    Science.gov (United States)

    Sadler, J.; Smith, M. I.

    2009-04-01

    The real-time fusion of imagery from two or more complementary sensors offers significant operational benefits for both operator-in-the-loop and automated processing systems. This paper reports on a new image fusion framework that can be used to maximise detection, recognition and identification performance within the context of low false-alarm rate operation. The Intelligent Image Fusion (I2F) architecture presented here allows exploitation of data at the information level as well as at the pixel-level, and can do so in an adaptable and intelligent manner. In this paper the architecture is examined in terms of design, applicability to a range of tasks, and performance factors such as adaptability, flexibility and utility. The relationship between algorithm design and hardware implementation, and the consequential impact on system performance, is also reviewed. Particular consideration is given to size, weight and power constraints that exist for some systems and their implications for processing optimisation and implementation on different processing platforms. Results are presented from the outcome of quantitative studies, development programmes and system trials.

  9. Energy enhancement for deuteron beam fast ignition of a precompressed inertial confinement fusion target

    International Nuclear Information System (INIS)

    Fast Ignition (FI) is recognized as a potentially promising approach to achieve the high-energy-gain target performance needed for commercial inertial confinement fusion. Here we consider deuteron beam driven FI which provides not only the 'hot spot' ignition spark, but also extra ''bonus'' fusion energy through reactions in the target. In this study, we estimate the impact of the added deposition energy due to the fusion reactions occurring, based on calculations using a modified energy multiplication factor Fc. The deuteron beam energy deposition range and time are also evaluated in order to estimate the desired deuteron initial energy. It is shown that an average of 30% extra energy can be gained from deuterons with 1 MeV initial energy and 12% from deuterons with 3 MeV initial energy. These results indicate that the energy benefit of this approach could be significant, but a much more comprehensive calculation is needed to realize a full 3D design for realistic experimental studies.

  10. FUSION OF VENTURI AND ULTRASONIC FLOW METER FOR ENHANCED FLOW METER CHARACTERISTICS USING FUZZY LOGIC

    OpenAIRE

    K.V. Santhosh

    2015-01-01

    This paper proposes a technique for measurement of liquid flow using venturi and ultrasonic flow meter(UFM) to have following objectives a) to design a multi-sensor data fusion (MSDF) architecture for using both the sensors, b) improve sensitivity and linearity of venturi and ultrasonic flow meter, and c) detect and diagnosis of faults in sensor if any. Fuzzy logic algorithm is used to fuse outputs of both the sensor and train the fuzzy block to produces output which has an improved character...

  11. Data Analysis Software Tools for Enhanced Collaboration at the DIII-D National Fusion Facility

    International Nuclear Information System (INIS)

    Data analysis at the DIII-D National Fusion Facility is simplified by the use of two software packages in analysis codes. The first is GAP1otObj, an IDL-based object-oriented library used in visualization tools for dynamic plotting. GAPlotObj gives users the ability to manipulate graphs directly through mouse and keyboard-driven commands. The second software package is MDSplus, which is used at DIED as a central repository for analyzed data. GAPlotObj and MDSplus reduce the effort required for a collaborator to become familiar with the DIII-D analysis environment by providing uniform interfaces for data display and retrieval. Two visualization tools at DIII-D that benefit from them are ReviewPlus and EFITviewer. ReviewPlus is capable of displaying interactive 2D and 3D graphs of raw, analyzed, and simulation code data. EFITviewer is used to display results from the EFIT analysis code together with kinetic profiles and machine geometry. Both bring new possibilities for data exploration to the user, and are able to plot data from any fusion research site with an MDSplus data server

  12. Feature level fusion for enhanced geological mapping of ophiolile complex using ASTER and Landsat TM data

    International Nuclear Information System (INIS)

    Chromite ore deposit occurrence is related to ophiolite complexes as a part of the oceanic crust and provides a good opportunity for lithological mapping using remote sensing data. The main contribution of this paper is a novel approaches to discriminate different rock units associated with ophiolite complex using the Feature Level Fusion technique on ASTER and Landsat TM satellite data at regional scale. In addition this study has applied spectral transform approaches, consisting of Spectral Angle Mapper (SAM) to distinguish the concentration of high-potential areas of chromite and also for determining the boundary between different rock units. Results indicated both approaches show superior outputs compared to other methods and can produce a geological map for ophiolite complex rock units in the arid and the semi-arid region. The novel technique including feature level fusion and Spectral Angle Mapper (SAM) discriminated ophiolitic rock units and produced detailed geological maps of the study area. As a case study, Sikhoran ophiolite complex located in SE, Iran has been selected for image processing techniques. In conclusion, a suitable approach for lithological mapping of ophiolite complexes is demonstrated, this technique contributes meaningfully towards economic geology in terms of identifying new prospects

  13. Enhanced arrangement for recuperators in supercritical CO2 Brayton power cycle for energy conversion in fusion reactors

    International Nuclear Information System (INIS)

    Highlights: •We propose an enhanced power conversion system layout for a Model C fusion reactor. •Proposed layout is based on a modified recompression supercritical CO2 Brayton cycle. •New arrangement in recuperators regards to classical cycle is used. •High efficiency is achieved, comparable with the best obtained in complex solutions. -- Abstract: A domestic research program called TECNOFUS was launched in Spain in 2009 to support technological developments related to a dual coolant breeding blanket concept for fusion reactors. This concept of blanket uses Helium (300 °C/400 °C) to cool part of it and a liquid metal (480 °C/700 °C) to cool the rest; it also includes high temperature (700 °C/800 °C) and medium temperature (566 °C/700 °C) Helium cooling circuits for divertor. This paper proposes a new layout of the classical recompression supercritical CO2 Brayton cycle which replaces one of the recuperators (the one with the highest temperature) by another which by-passes the low temperature blanket source. This arrangement allows reaching high turbine inlet temperatures (around 600 °C) with medium pressures (around 225 bar) and achieving high cycle efficiencies (close to 46.5%). So, the proposed cycle reveals as a promising design because it integrates all the available thermal sources in a compact layout achieving high efficiencies with the usual parameters prescribed in classical recompression supercritical CO2 Brayton cycles

  14. Atomic and plasma-material interaction data for fusion. V. 7, part B. Particle induced erosion of Be, C and W in fusion plasmas. Part B: Physical sputtering and radiation-enhanced sublimation

    International Nuclear Information System (INIS)

    The present volume of Atomic and Plasma-Material Interaction Data for Fusion is devoted to a critical review of the physical sputtering and radiation enhanced sublimation (RES) behaviour of fusion plasma-facing materials, in particular carbon, beryllium and tungsten. The present volume is intended to provide fusion reactor designers a detailed survey and parameterization of existing, critically assessed data for the chemical erosion of plasma-facing materials by particle impact. The survey and data compilation is presented for a variety of materials containing the elements C, Be and W (including dopants in carbon materials) and impacting plasma species. The dependencies of physical sputtering and RES yields on the material temperature, incident projectile energy, and incident flux are considered. The main data compilation is presented as separate data sheets indicating the material, impacting plasma species, experimental conditions, and parameterizations in terms of analytic functions

  15. Construction and evaluation of an exopolysaccharide-producing engineered bacterial strain by protoplast fusion for microbial enhanced oil recovery.

    Science.gov (United States)

    Sun, Shanshan; Luo, Yijing; Cao, Siyuan; Li, Wenhong; Zhang, Zhongzhi; Jiang, Lingxi; Dong, Hanping; Yu, Li; Wu, Wei-Min

    2013-09-01

    Enterobacter cloacae strain JD, which produces water-insoluble biopolymers at optimal temperature of 30°C, and a thermophilic Geobacillus strain were used to construct an engineered strain for exopolysaccharide production at high temperatures by protoplast fusion. The obtained fusant strain ZR3 produced exopolysaccharides at up to 45°C with optimal growth temperature at 35°C. The fusant produced exopolysaccharides of approximately 7.5 g/L or more at pH between 7.0 and 9.0. The feasibility of the enhancement of crude oil recovery with the fusant was tested in a sand-packed column at 40°C. The results demonstrated that bioaugmentation of the fusant was promising approach for MEOR. Mass growth of the fusant was confirmed in fermentor tests. PMID:23856587

  16. Anomalous enhancements of low-energy fusion rates in plasmas: the role of ion momentum distributions and inhomogeneous screening

    Science.gov (United States)

    Coraddu, Massimo; Lissia, Marcello; Quarati, Piero

    2009-09-01

    Non-resonant fusion cross-sections significantly higher than corresponding theoretical predictions are observed in low-energy experiments with deuterated matrix target. Models based on thermal effects, electron screening, or quantum-effect dispersion relations have been proposed to explain these anomalous results: none of them appears to satisfactory reproduce the experiments. Velocity distributions are fundamental for the reaction rates and deviations from the Maxwellian limit could play a central role in explaining the enhancement. We examine two effects: an increase of the tail of the target Deuteron momentum distribution due to the Galitskii-Yakimets quantum uncertainty effect, which broadens the energy-momentum relation; and spatial fluctuations of the Debye-Hückel radius leading to an effective increase of electron screening. Either effect leads to larger reaction rates especially large at energies below a few keV, reducing the discrepancy between observations and theoretical expectations.

  17. Anomalous enhancements of low-energy fusion rates in plasmas: the role of ion momentum distributions and inhomogeneous screening

    CERN Document Server

    Coraddu, M; Quarati, P

    2009-01-01

    Non-resonant fusion cross-sections significantly higher than corresponding theoretical predictions are observed in low-energy experiments with deuterated matrix target. Models based on thermal effects, electron screening, or quantum-effect dispersion relations have been proposed to explain these anomalous results: none of them appears to satisfactory reproduce the experiments. Velocity distributions are fundamental for the reaction rates and deviations from the Maxwellian limit could play a central role in explaining the enhancement. We examine two effects: an increase of the tail of the target Deuteron momentum distribution due to the Galitskii-Yakimets quantum uncertainty effect, which broadens the energy-momentum relation; and spatial fluctuations of the Debye-H\\"{u}ckel radius leading to an effective increase of electron screening. Either effect leads to larger reaction rates especially large at energies below a few keV, reducing the discrepancy between observations and theoretical expectations.

  18. FUSION OF VENTURI AND ULTRASONIC FLOW METER FOR ENHANCED FLOW METER CHARACTERISTICS USING FUZZY LOGIC

    Directory of Open Access Journals (Sweden)

    K.V. Santhosh

    2015-04-01

    Full Text Available This paper proposes a technique for measurement of liquid flow using venturi and ultrasonic flow meter(UFM to have following objectives a to design a multi-sensor data fusion (MSDF architecture for using both the sensors, b improve sensitivity and linearity of venturi and ultrasonic flow meter, and c detect and diagnosis of faults in sensor if any. Fuzzy logic algorithm is used to fuse outputs of both the sensor and train the fuzzy block to produces output which has an improved characteristics in terms of both sensitivity and linearity. For identification of sensor faults a comparative test algorithm is designed. Once trained proposed technique is tested in real life, results show successful implementation of proposed objectives.

  19. Enhancement of the influenza A hemagglutinin (HA-mediated cell-cell fusion and virus entry by the viral neuraminidase (NA.

    Directory of Open Access Journals (Sweden)

    Bin Su

    Full Text Available BACKGROUND: The major role of the neuraminidase (NA protein of influenza A virus is related to its sialidase activity, which disrupts the interaction between the envelope hemagglutinin (HA protein and the sialic acid receptors expressed at the surface of infected cells. This enzymatic activity is known to promote the release and spread of progeny viral particles following their production by infected cells, but a potential role of NA in earlier steps of the viral life cycle has never been clearly demonstrated. In this study we have examined the impact of NA expression on influenza HA-mediated viral membrane fusion and virion infectivity. METHODOLOGY/PRINCIPAL FINDINGS: The role of NA in the early stages of influenza virus replication was examined using a cell-cell fusion assay that mimics HA-mediated membrane fusion, and a virion infectivity assay using HIV-based pseudoparticles expressing influenza HA and/or NA proteins. In the cell-cell fusion assay, which bypasses the endocytocytosis step that is characteristic of influenza virus entry, we found that in proper HA maturation conditions, NA clearly enhanced fusion in a dose-dependent manner. Similarly, expression of NA at the surface of pseudoparticles significantly enhanced virion infectivity. Further experiments using exogenous soluble NA revealed that the most likely mechanism for enhancement of fusion and infectivity by NA was related to desialylation of virion-expressed HA. CONCLUSION/SIGNIFICANCE: The NA protein of influenza A virus is not only required for virion release and spread but also plays a critical role in virion infectivity and HA-mediated membrane fusion.

  20. Edge preserved enhancement of medical images using adaptive fusion-based denoising by shearlet transform and total variation algorithm

    Science.gov (United States)

    Gupta, Deep; Anand, Radhey Shyam; Tyagi, Barjeev

    2013-10-01

    Edge preserved enhancement is of great interest in medical images. Noise present in medical images affects the quality, contrast resolution, and most importantly, texture information and can make post-processing difficult also. An enhancement approach using an adaptive fusion algorithm is proposed which utilizes the features of shearlet transform (ST) and total variation (TV) approach. In the proposed method, three different denoised images processed with TV method, shearlet denoising, and edge information recovered from the remnant of the TV method and processed with the ST are fused adaptively. The result of enhanced images processed with the proposed method helps to improve the visibility and detectability of medical images. For the proposed method, different weights are evaluated from the different variance maps of individual denoised image and the edge extracted information from the remnant of the TV approach. The performance of the proposed method is evaluated by conducting various experiments on both the standard images and different medical images such as computed tomography, magnetic resonance, and ultrasound. Experiments show that the proposed method provides an improvement not only in noise reduction but also in the preservation of more edges and image details as compared to the others.

  1. Enhancing chemical identification efficiency by SAW sensor transients through a data enrichment and information fusion strategy—a simulation study

    International Nuclear Information System (INIS)

    The paper proposes a new approach for improving the odor recognition efficiency of a surface acoustic wave (SAW) transient sensor system based on a single polymer coating. The vapor identity information is hidden in transient response shapes through dependences on specific vapor solvation and diffusion parameters in the polymer coating. The variations in the vapor exposure and purge durations and the sensor operating frequency have been used to create diversity in transient shapes via termination of the vapor–polymer equilibration process up to different stages. The transient signals were analyzed by the discrete wavelet transform using Daubechies-4 mother wavelet basis. The wavelet approximation coefficients were then processed by principal component analysis for creating feature space. The set of principal components define the vapor identity information. In an attempt to enhance vapor class separability we analyze two types of information fusion methods. In one, the sensor operation frequency is fixed and the sensing and purge durations are varied, and in the second, the sensing and purge durations are fixed and the sensor operating frequency is varied. The fusion is achieved by concatenation of discrete wavelet coefficients corresponding to various transients prior to the principal component analysis. The simulation experiments with polyisobutylene SAW sensor coating for operation frequencies over [55–160] MHz and sensing durations over [5–60] s were analyzed. The target vapors are seven volatile organics: chloroform, chlorobenzene, o-dichlorobenzene, n-heptane, toluene, n-hexane and n-octane whose concentrations were varied over [10–100] ppm. The simulation data were generated using a SAW sensor transient response model that incorporates the viscoelastic effects due to polymer coating and an additive noise source in the output. The analysis reveals that: (i) in single transient analysis the class separability increases with sensing duration for a given

  2. Uniaxial cyclic strain enhances adipose-derived stem cell fusion with skeletal myocytes

    Energy Technology Data Exchange (ETDEWEB)

    Andersen, Jens Isak; Juhl, Morten; Nielsen, Thøger; Emmersen, Jeppe; Fink, Trine; Zachar, Vladimir; Pennisi, Cristian Pablo, E-mail: cpennisi@hst.aau.dk

    2014-07-25

    Highlights: • Uniaxial cyclic tensile strain (CTS) applied to ASCs alone or in coculture with myogenic precursors. • CTS promoted the formation of a highly ordered array of parallel ASCs. • Without biochemical supplements, CTS did not support advanced myogenic differentiation of ASCs. • Mechanical stimulation of cocultures boosted fusion of ASCs with skeletal myoblasts. - Abstract: Although adult muscle tissue possesses an exceptional capacity for regeneration, in the case of large defects, the restoration to original state is not possible. A well-known source for the de novo regeneration is the adipose-derived stem cells (ASCs), which can be readily isolated and have been shown to have a broad differentiation and regenerative potential. In this work, we employed uniaxial cyclic tensile strain (CTS), to mechanically stimulate human ASCs to participate in the formation skeletal myotubes in an in vitro model of myogenesis. The application of CTS for 48 h resulted in the formation of a highly ordered array of parallel ASCs, but failed to support skeletal muscle terminal differentiation. When the same stimulation paradigm was applied to cocultures with mouse skeletal muscle myoblasts, the percentage of ASCs contributing to the formation of myotubes significantly exceeded the levels reported in the literature hitherto. In perspective, the mechanical strain may be used to increase the efficiency of incorporation of ASCs in the skeletal muscles, which could be found useful in diverse traumatic or pathologic scenarios.

  3. Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture.

    Science.gov (United States)

    Hu, Dongdong; Sun, Yating; Liu, Xuping; Liu, Jintao; Zhang, Xintao; Zhao, Liang; Wang, Haibin; Tan, Wen-Song; Fan, Li

    2015-10-01

    Yeast extract (YE), as a non-animal source additive for mammalian cell culture medium, has been widely used for manufacturing of therapeutic proteins. In the present study, one particular YE was found to have significantly improved the specific productivity (q p) of Fc-fusion protein in recombinant Chinese hamster ovary (rCHO) cell culture. In order to elucidate the intracellular effects of YE on protein productivity, steps of the target protein synthesis process were investigated to unveil their variations caused by YE addition. Stepwise analysis on Fc-fusion protein synthesis process showed that YE enhanced Fc-fusion protein gene transcription with cell cycle arrest at G1 phase; mammalian target of rapamycin (mTOR) signaling pathway was activated to enhance the translation of Fc-fusion protein, and the block in post-translational steps of Fc-fusion protein was alleviated by YE addition as well. Our results revealed the responses of multiple protein production steps to the addition of YE and provided a practical guidance for the separation and application of active compounds from hydrolysates. PMID:26162671

  4. Sub-barrier enhancement of fusion as compared to a microscopic method in 18O+12C

    OpenAIRE

    Steinbach, T. K.; Vadas, J.; Schmidt, J.; Haycraft, C.; Hudan, S.; deSouza, R. T.; Baby, L. T.; Kuvin, S. A.; Wiedenhöver, I.; Umar, A. S.; Oberacker, V. E.

    2014-01-01

    Measurement of the energy dependence of the fusion cross-sec on at sub-barrier energies provides an important test for theoretical models of fusion. To extend the measurement of fusion cross-sections in the sub-barrier domain for the 18O+12C system. Use the new experimental data to confront microscopic calculations of fusion. Evaporation residues produced in fusion of 18O ions with 12C target nuclei were detected with good geometric efficiency and identified by measuring their energy and time...

  5. Development and Application of Non-Linear Image Enhancement and Multi-Sensor Fusion Techniques for Hazy and Dark Imaging

    Science.gov (United States)

    Rahman, Zia-ur

    2005-01-01

    The purpose of this research was to develop enhancement and multi-sensor fusion algorithms and techniques to make it safer for the pilot to fly in what would normally be considered Instrument Flight Rules (IFR) conditions, where pilot visibility is severely restricted due to fog, haze or other weather phenomenon. We proposed to use the non-linear Multiscale Retinex (MSR) as the basic driver for developing an integrated enhancement and fusion engine. When we started this research, the MSR was being applied primarily to grayscale imagery such as medical images, or to three-band color imagery, such as that produced in consumer photography: it was not, however, being applied to other imagery such as that produced by infrared image sources. However, we felt that it was possible by using the MSR algorithm in conjunction with multiple imaging modalities such as long-wave infrared (LWIR), short-wave infrared (SWIR), and visible spectrum (VIS), we could substantially improve over the then state-of-the-art enhancement algorithms, especially in poor visibility conditions. We proposed the following tasks: 1) Investigate the effects of applying the MSR to LWIR and SWIR images. This consisted of optimizing the algorithm in terms of surround scales, and weights for these spectral bands; 2) Fusing the LWIR and SWIR images with the VIS images using the MSR framework to determine the best possible representation of the desired features; 3) Evaluating different mixes of LWIR, SWIR and VIS bands for maximum fog and haze reduction, and low light level compensation; 4) Modifying the existing algorithms to work with video sequences. Over the course of the 3 year research period, we were able to accomplish these tasks and report on them at various internal presentations at NASA Langley Research Center, and in presentations and publications elsewhere. A description of the work performed under the tasks is provided in Section 2. The complete list of relevant publications during the research

  6. Support vector data description for fusion of multiple health indicators for enhancing gearbox fault diagnosis and prognosis

    International Nuclear Information System (INIS)

    A novel method for enhancing gearbox fault diagnosis and prognosis is developed by fusion of multiple health indicators through support vector data description. First, the Comblet transform is used to identify gear residual error signals from the raw signal. Second, based on the observation of gear residual error signals, a total of 11 gear health indicators are identified, and are categorized into two types of indicators. The first and second types of indicators are for fault diagnosis and prognosis, respectively. The first type has six indicators, which are sensitive to impulsive signals triggered by anomalous impacts. The second type has five indicators, which are suitable for tracking degradation of faults. Third, through the support vector data description, the first six health indicators are fused into type one indicators for fault diagnosis. The remaining five indicators are fused into type two indicators for fault prognosis. Finally, a Gaussian kernel is designed to enhance the performance of type one and two indicators by optimal range of width size. The effectiveness of the proposed method is validated through experiments. The new method has been proven to be superior to methods that use unfused indicators individually

  7. Recent enhancements to analyzed data acquisition and remote participation at the DIII-D National Fusion Facility

    International Nuclear Information System (INIS)

    A long-term plan is being implemented to enhance the computational infrastructure of the DIII-D National Fusion Facility. One of the goals of this plan is more efficient utilization of DIII-D experimental run-time by decreasing the time to analyze, store and distribute analyzed data during tokamak operations. A multi-processor Linux cluster is reducing data analysis time and a Unix based MDSplus data management system is providing rapid access to analyzed data. A second goal of the long-term plan is to reduce the time required for more detailed physics analysis after experimental operations. This goal is being accomplished with the underlying philosophy of uniformity, both in the look and feel of our own GUI-based tools, in terms of access methods to analyzed datasets, and access to existing computer power via a load balanced UNIX cluster. Additionally, we have enhanced our remote meeting capability resulting in improved communication within the geographically diverse DIII-D Research Team

  8. Experimental setup for producing tungsten coated graphite tiles using plasma enhanced chemical vapor deposition technique for fusion plasma applications

    International Nuclear Information System (INIS)

    Plasma wall interaction (PWI) in fusion grade machines puts stringent demands on the choice of materials in terms of high heat load handling capabilities and low sputtering yields. Choice of suitable material still remains a challenge and open topic of research for the PWI community. Carbon fibre composites (CFC), Beryllium (Be), and Tungsten (W) are now being considered as first runners for the first wall components of future fusion machines. Tungsten is considered to be one of the suitable materials for the job because of its superior properties than carbon like low physical sputtering yield and high sputter energy threshold, high melting point, fairly high re-crystallization temperature, low fuel retention capabilities, low chemical sputtering with hydrogen and its isotopes and most importantly the reparability with various plasma techniques both ex-situ and in-situ. Plasma assisted chemical vapour deposition is considered among various techniques as the most preferable technique for fabricating tungsten coated graphite tiles to be used as tokamak first wall and target components. These coated tiles are more favourable compared to pure tungsten due to their light weight and easier machining. A system has been designed, fabricated and installed at SVITS, Indore for producing tungsten coated graphite tiles using Plasma Enhanced Chemical Vapor Deposition (PE-CVD) technique for Fusion plasma applications. The system contains a vacuum chamber, a turbo-molecular pump, two electrodes, vacuum gauges, mass analyzer, mass flow controllers and a RF power supply for producing the plasma using hydrogen gas. The graphite tiles will be put on one of the electrodes and WF6 gas will be inserted in a controlled manner in the hydrogen plasma to achieve the tungsten-coating with WF6 dissociation. The system is integrated at SVITS, Indore and a vacuum of the order of 3*10-6 is achieved and glow discharge plasma has been created to test all the sub-systems. The system design with all

  9. Myxoma virus expressing a fusion protein of interleukin-15 (IL15 and IL15 receptor alpha has enhanced antitumor activity.

    Directory of Open Access Journals (Sweden)

    Vesna Tosic

    Full Text Available Myxoma virus, a rabbit poxvirus, can efficiently infect various types of mouse and human cancer cells. It is a strict rabbit-specific pathogen, and is thought to be safe as a therapeutic agent in all non-rabbit hosts tested including mice and humans. Interleukin-15 (IL15 is an immuno-modulatory cytokine with significant potential for stimulating anti-tumor T lymphocytes and NK cells. Co-expression of IL15 with the α subunit of IL15 receptor (IL15Rα greatly enhances IL15 stability and bioavailability. Therefore, we engineered a new recombinant myxoma virus (vMyx-IL15Rα-tdTr, which expresses an IL15Rα-IL15 fusion protein plus tdTomato red fluorescent reporter protein. Permissive rabbit kidney epithelial (RK-13 cells infected with vMyx-IL15Rα-tdTr expressed and secreted the IL15Rα-IL15 fusion protein. Functional activity was confirmed by demonstrating that the secreted fusion protein stimulated proliferation of cytokine-dependent CTLL-2 cells. Multi-step growth curves showed that murine melanoma (B16-F10 and B16.SIY cell lines were permissive to vMyx-IL15Rα-tdTr infection. In vivo experiments in RAG1-/- mice showed that subcutaneous B16-F10 tumors treated with vMyx-IL15Rα-tdTr exhibited attenuated tumor growth and a significant survival benefit for the treated group compared to the PBS control and the control viruses (vMyx-IL15-tdTr and vMyx-tdTr. Immunohistological analysis of the subcutaneous tumors showed dramatically increased infiltration of NK cells in vMyx-IL15Rα-tdTr treated tumors compared to the controls. In vivo experiments with immunocompetent C57BL/6 mice revealed a strong infiltrate of both NK cells and CD8+ T cells in response to vMyx-IL15Rα-tdTr, and prolonged survival. We conclude that delivery of IL15Rα-IL15 in a myxoma virus vector stimulates both innate and adaptive components of the immune system.

  10. Complex-I Alteration and Enhanced Mitochondrial Fusion Are Associated With Prostate Cancer Progression.

    Science.gov (United States)

    Philley, Julie V; Kannan, Anbarasu; Qin, Wenyi; Sauter, Edward R; Ikebe, Mitsuo; Hertweck, Kate L; Troyer, Dean A; Semmes, Oliver J; Dasgupta, Santanu

    2016-06-01

    Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa and assessed the impact of mtDNA depletion on mt integrity. We also examined whether the circulating exosomes from PCa patients are transported to mt and carry mtDNA or mt proteins. We have employed next generation sequencing of the whole mt genome in human and Hi-myc PCa. The impact of mtDNA depletion on mt integrity, presence of mtDNA, and protein in sera exosomes was determined. A co-culture of human PCa cells and the circulating exosomes followed by confocal imaging determined co-localization of exosomes and mt. We observed frequent RCI mutations in human and Hi-myc PCa which disrupted corresponding complex protein expression. Depletion of mtDNA in PCa cells influenced mt integrity, increased expression of MFN1, MFN2, PINK1, and decreased expression of MT-TFA. Increased mt fusion and expression of PINK1 and DNM1L were also evident in the Hi-myc tumors. RCI-mtDNA, MFN2, and IMMT proteins were detected in the circulating exosomes of men with benign prostate hyperplasia (BPH) and progressive PCa. Circulating exosomes and mt co-localized in PCa cells. Our study identified new pathogenic RCI mutations in PCa and defined the impact of mtDNA loss on mt integrity. Presence of mtDNA and mt proteins in the circulating exosomes implicated their usefulness for biomarker development. PMID:26530043

  11. Inertial confinement fusion driver enhancements: Final focusing systems and compact heavy-ion driver designs

    International Nuclear Information System (INIS)

    Required elements of an inertial confinement fusion power plant are modeled and discussed. A detailed analysis of two critical elements of candidate drivers is done, and new component designs are proposed to increase the credibility and feasibility of each driver system. An analysis of neutron damage to the final elements of a laser focusing system is presented, and multilayer -- dielectric mirrors are shown to have damage lifetimes which axe too short to be useful in a commercial power plant. A new final-focusing system using grazing incidence metal mirrors to protect sensitive laser optics is designed and shown to be effective in extending the lifetime of the final focusing system. The reflectivities and damage limits of grazing incidence metal mirrors are examined in detail, and the required mirror sizes are shown to be compatible with the beam sizes and illumination geometries currently envisioned for laser drivers. A detailed design and analysis is also done for compact arrays of superconducting magnetic quadrupoles, which are needed in a multi-beam heavy-ion driver. The new array model is developed in more detail than some previous conceptual designs and models arrays which are more compact than arrays scaled from existing single -- quadrupole designs. The improved integrated model for compact arrays is used to compare the effects of various quadrupole array design choices on the size and cost of a heavy-ion driver. Array design choices which significantly affect the cost of a heavy-ion driver include the choice of superconducting material and the thickness of the collar used to support the winding stresses. The effect of these array design choices on driver size and cost is examined and the array model is used to estimate driver cost savings and performance improvements attainable with aggressive quadrupole array designs with high-performance superconductors

  12. Enhancement of plaque formation and cell fusion of an enteropathogenic coronavirus by trypsin treatment.

    OpenAIRE

    Storz, J.; Rott, R; Kaluza, G.

    1981-01-01

    Plaque formation, replication, and related cytopathic functions of the enteropathogenic bovine coronavirus strain L9 in bovine fetal thyroid (BFTy) and bovine fetal brain (BFB) cells were investigated in the presence and absence of trypsin. Plaque formation was enhanced in both cell types. Plaques reached a size with an average diameter of 5 mm within 4 days with trypsin in the overlay, whereas their diameter remained less than 1 mm at this time after plating without trypsin in the overlay. F...

  13. APC targeting enhances immunogenicity of a novel multistage Fc-fusion tuberculosis vaccine in mice.

    Science.gov (United States)

    Soleimanpour, Saman; Farsiani, Hadi; Mosavat, Arman; Ghazvini, Kiarash; Eydgahi, Mohammad Reza Akbari; Sankian, Mojtaba; Sadeghian, Hamid; Meshkat, Zahra; Rezaee, Seyed Abdolrahim

    2015-12-01

    Numerous studies have demonstrated that targeting immunogens to FcγR on antigen-presenting cells (APCs) can selectively uptake and increase cellular immunity in vitro and in vivo. Therefore, the present study was conducted to evaluate immunogenicity of a novel multistage tuberculosis vaccine, a combination of an early and a dormant immunogenic protein, ESAT6 and HspX, fused to Fcγ2a fragment of mouse IgG2a to target all forms of tuberculosis. Codon-optimized genes consisting of ESAT6, a linker, and HspX fused either to mouse Fcγ2a (ESAT6:HspX:mFcγ2a) or 6× His-tag (ESAT6:HspX:His) were synthesized. The resulting proteins were then produced in Pichia pastoris. The fusion proteins were separately emulsified in dimethyldioctadecylammonium bromide(DDA)-trehalose-6,6-dibehenate(TDB) adjuvant, and their immunogenicity with and without bacille Calmette-Guérin (BCG) was assessed in C57BL/6 mice. Th1, Th2, Th17, and T-reg cytokine patterns were evaluated using the ELISA method. Both multistage vaccines induced very strong IL-12 and IFN-γ secretion from splenic cells; the Fc-tagged subunit vaccine induced a more effective Th1 immune response (IFN-γ, 910 pg/mL, and IL-12, 854 pg/mL) with a very low increase in IL-17 (∼0.1 pg/mL) and IL-4 (37 pg/mL) and a mild increase in TGF-β (543 pg/mL) compared to the BCG or ESAT6:HspX:His primed and boosted groups. The production of IFN-γ to ESAT6:HspX:Fcγ2a was very consistent and showed an increasing trend for IL-12 compared to the BCG or ESAT6:HspX:His primed and boosted groups. Fcγ2a used as a delivery vehicle supported the idea of selective uptake, inducing cross-presentation and forming a proper anti-tuberculosis response in context of Th1/Th2 and Th17/T-reg balances, which is important for protection and prevention of damage. PMID:26373723

  14. Robust electromagnetically guided endoscopic procedure using enhanced particle swarm optimization for multimodal information fusion

    International Nuclear Information System (INIS)

    Purpose: Electromagnetically guided endoscopic procedure, which aims at accurately and robustly localizing the endoscope, involves multimodal sensory information during interventions. However, it still remains challenging in how to integrate these information for precise and stable endoscopic guidance. To tackle such a challenge, this paper proposes a new framework on the basis of an enhanced particle swarm optimization method to effectively fuse these information for accurate and continuous endoscope localization. Methods: The authors use the particle swarm optimization method, which is one of stochastic evolutionary computation algorithms, to effectively fuse the multimodal information including preoperative information (i.e., computed tomography images) as a frame of reference, endoscopic camera videos, and positional sensor measurements (i.e., electromagnetic sensor outputs). Since the evolutionary computation method usually limits its possible premature convergence and evolutionary factors, the authors introduce the current (endoscopic camera and electromagnetic sensor’s) observation to boost the particle swarm optimization and also adaptively update evolutionary parameters in accordance with spatial constraints and the current observation, resulting in advantageous performance in the enhanced algorithm. Results: The experimental results demonstrate that the authors’ proposed method provides a more accurate and robust endoscopic guidance framework than state-of-the-art methods. The average guidance accuracy of the authors’ framework was about 3.0 mm and 5.6° while the previous methods show at least 3.9 mm and 7.0°. The average position and orientation smoothness of their method was 1.0 mm and 1.6°, which is significantly better than the other methods at least with (2.0 mm and 2.6°). Additionally, the average visual quality of the endoscopic guidance was improved to 0.29. Conclusions: A robust electromagnetically guided endoscopy framework was

  15. Robust electromagnetically guided endoscopic procedure using enhanced particle swarm optimization for multimodal information fusion

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Xiongbiao, E-mail: xluo@robarts.ca, E-mail: Ying.Wan@student.uts.edu.au [Robarts Research Institute, Western University, London, Ontario N6A 5K8 (Canada); Wan, Ying, E-mail: xluo@robarts.ca, E-mail: Ying.Wan@student.uts.edu.au; He, Xiangjian [School of Computing and Communications, University of Technology, Sydney, New South Wales 2007 (Australia)

    2015-04-15

    Purpose: Electromagnetically guided endoscopic procedure, which aims at accurately and robustly localizing the endoscope, involves multimodal sensory information during interventions. However, it still remains challenging in how to integrate these information for precise and stable endoscopic guidance. To tackle such a challenge, this paper proposes a new framework on the basis of an enhanced particle swarm optimization method to effectively fuse these information for accurate and continuous endoscope localization. Methods: The authors use the particle swarm optimization method, which is one of stochastic evolutionary computation algorithms, to effectively fuse the multimodal information including preoperative information (i.e., computed tomography images) as a frame of reference, endoscopic camera videos, and positional sensor measurements (i.e., electromagnetic sensor outputs). Since the evolutionary computation method usually limits its possible premature convergence and evolutionary factors, the authors introduce the current (endoscopic camera and electromagnetic sensor’s) observation to boost the particle swarm optimization and also adaptively update evolutionary parameters in accordance with spatial constraints and the current observation, resulting in advantageous performance in the enhanced algorithm. Results: The experimental results demonstrate that the authors’ proposed method provides a more accurate and robust endoscopic guidance framework than state-of-the-art methods. The average guidance accuracy of the authors’ framework was about 3.0 mm and 5.6° while the previous methods show at least 3.9 mm and 7.0°. The average position and orientation smoothness of their method was 1.0 mm and 1.6°, which is significantly better than the other methods at least with (2.0 mm and 2.6°). Additionally, the average visual quality of the endoscopic guidance was improved to 0.29. Conclusions: A robust electromagnetically guided endoscopy framework was

  16. Data management, code deployment, and scientific visualization to enhance scientific discovery in fusion research through advanced computing

    International Nuclear Information System (INIS)

    The long-term vision of the Fusion Collaboratory described in this paper is to transform fusion research and accelerate scientific understanding and innovation so as to revolutionize the design of a fusion energy source. The Collaboratory will create and deploy collaborative software tools that will enable more efficient utilization of existing experimental facilities and more effective integration of experiment, theory, and modeling. The computer science research necessary to create the Collaboratory is centered on three activities: security, remote and distributed computing, and scientific visualization. It is anticipated that the presently envisioned Fusion Collaboratory software tools will require 3 years to complete

  17. Neutron yield enhancement in laser-induced deuterium-deuterium fusion using a novel shaped target

    International Nuclear Information System (INIS)

    Neutron yields have direct correlation with the energy of incident deuterons in experiments of laser deuterated target interaction [Roth et al., Phys. Rev. Lett. 110, 044802 (2013) and Higginson et al., Phys. Plasmas 18, 100703 (2011)], while deuterated plasma density is also an important parameter. Experiments at the Shenguang II laser facility have produced neutrons with energy of 2.45 MeV using d (d, n) He reaction. Deuterated foil target and K-shaped target were employed to study the influence of plasma density on neutron yields. Neutron yield generated by K-shaped target (nearly 106) was two times higher than by foil target because the K-shaped target results in higher density plasma. Interferometry and multi hydro-dynamics simulation confirmed the importance of plasma density for enhancement of neutron yields

  18. Human VE-Cadherin Fusion Protein as an Artificial Extracellular Matrix Enhancing the Proliferation and Differentiation Functions of Endothelial Cell.

    Science.gov (United States)

    Xu, Ke; Shuai, Qizhi; Li, Xiaoning; Zhang, Yan; Gao, Chao; Cao, Lei; Hu, Feifei; Akaike, Toshihiro; Wang, Jian-Xi; Gu, Zhongwei; Yang, Jun

    2016-03-14

    In an attempt to enhance endothelial cell capture and promote the vascularization of engineered tissue, we biosynthesized and characterized the recombinant fusion protein consisting of human vascular endothelial-cadherin extracellular domain and immunoglobulin IgG Fc region (hVE-cad-Fc) to serve as a bioartificial extracellular matrix. The hVE-cad-Fc protein naturally formed homodimers and was used to construct hVE-cad-Fc matrix by stably adsorbing on polystyrene plates. Atomic force microscop assay showed uniform hVE-cad-Fc distribution with nanorod topography. The hVE-cad-Fc matrix markedly promoted human umbilical vein endothelial cells (HUVECs) adhesion and proliferation with fibroblastoid morphology. Additionally, the hVE-cad-Fc matrix improved HUVECs migration, vWF expression, and NO release, which are closely related to vascularization. Furthermore, the hVE-cad-Fc matrix activated endogenous VE-cadherin/β-catenin proteins and effectively triggered the intracellular signals such as F-actin stress fiber, p-FAK, AKT, and Bcl-2. Taken together, hVE-cad-Fc could be a promising bioartificial matrix to promote vascularization in tissue engineering. PMID:26859785

  19. Enhanced vaccine-induced CD8+ T cell responses to malaria antigen ME-TRAP by fusion to MHC class ii invariant chain.

    Directory of Open Access Journals (Sweden)

    Alexandra J Spencer

    Full Text Available The orthodox role of the invariant chain (CD74; Ii is in antigen presentation to CD4+ T cells, but enhanced CD8+ T cells responses have been reported after vaccination with vectored viral vaccines encoding a fusion of Ii to the antigen of interest. In this study we assessed whether fusion of the malarial antigen, ME-TRAP, to Ii could increase the vaccine-induced CD8+ T cell response. Following single or heterologous prime-boost vaccination of mice with a recombinant chimpanzee adenovirus vector, ChAd63, or recombinant modified vaccinia virus Ankara (MVA, higher frequencies of antigen-specific CD4+ and CD8+ T cells were observed, with the largest increases observed following a ChAd63-MVA heterologous prime-boost regimen. Studies in non-human primates confirmed the ability of Ii-fusion to augment the T cell response, where a 4-fold increase was maintained up to 11 weeks after the MVA boost. Of the numerous different approaches explored to increase vectored vaccine induced immunogenicity over the years, fusion to the invariant chain showed a consistent enhancement in CD8+ T cell responses across different animal species and may therefore find application in the development of vaccines against human malaria and other diseases where high levels of cell-mediated immunity are required.

  20. Multi-Sensor Fusion for Enhanced Contextual Awareness of Everyday Activities with Ubiquitous Devices

    Directory of Open Access Journals (Sweden)

    John J. Guiry

    2014-03-01

    Full Text Available In this paper, the authors investigate the role that smart devices, including smartphones and smartwatches, can play in identifying activities of daily living. A feasibility study involving N = 10 participants was carried out to evaluate the devices’ ability to differentiate between nine everyday activities. The activities examined include walking, running, cycling, standing, sitting, elevator ascents, elevator descents, stair ascents and stair descents. The authors also evaluated the ability of these devices to differentiate indoors from outdoors, with the aim of enhancing contextual awareness. Data from this study was used to train and test five well known machine learning algorithms: C4.5, CART, Naïve Bayes, Multi-Layer Perceptrons and finally Support Vector Machines. Both single and multi-sensor approaches were examined to better understand the role each sensor in the device can play in unobtrusive activity recognition. The authors found overall results to be promising, with some models correctly classifying up to 100% of all instances.

  1. Genetic interaction between Tmprss2-ERG gene fusion and Nkx3.1-loss does not enhance prostate tumorigenesis in mouse models.

    Directory of Open Access Journals (Sweden)

    Douglas E Linn

    Full Text Available Gene fusions involving ETS family transcription factors (mainly TMPRSS2-ERG and TMPRSS2-ETV1 fusions have been found in ~50% of human prostate cancer cases. Although expression of TMPRSS2-ERG or TMPRSS2-ETV1 fusion alone is insufficient to initiate prostate tumorigenesis, they appear to sensitize prostate epithelial cells for cooperation with additional oncogenic mutations to drive frank prostate adenocarcinoma. To search for such ETS-cooperating oncogenic events, we focused on a well-studied prostate tumor suppressor NKX3.1, as loss of NKX3.1 is another common genetic alteration in human prostate cancer. Previous studies have shown that deletions at 8p21 (harboring NKX3.1 and 21q22 (resulting in TMPRSS2-ERG fusion were both present in a subtype of prostate cancer cases, and that ERG can lead to epigenetic silencing of NKX3.1 in prostate cancer cells, whereas NKX3.1 can in turn negatively regulate TMPRSS2-ERG fusion expression via suppression of the TMPRSS2 promoter activity. We recently generated knockin mouse models for TMPRSS2-ERG and TMPRSS2-ETV1 fusions, utilizing the endogenous Tmprss2 promoter. We crossed these knockin models to an Nkx3.1 knockout mouse model. In Tmprss2-ERG;Nkx3.1+/- (or -/- male mice, although we observed a slight but significant upregulation of Tmprss2-ERG fusion expression upon Nkx3.1 loss, we did not detect any significant cooperation between these two genetic events to enhance prostate tumorigenesis in vivo. Furthermore, retrospective analysis of a previously published human prostate cancer dataset revealed that within ERG-overexpressing prostate cancer cases, NKX3.1 loss or deletion did not predict biochemical relapse after radical prostatectomy. Collectively, these data suggest that although TMPRSS2-ERG fusion and loss of NKX3.1 are among the most common mutational events found in prostate cancer, and although each of them can sensitize prostate epithelial cells for cooperating with other oncogenic events, these

  2. Evaluation of cell binding peptide (p15) with silk fibre enhanced hydroxyappatite bone substitute for posterolateral spinal fusion in sheep

    DEFF Research Database (Denmark)

    Axelsen, M.; Jespersen, Stig; Overgaard, Søren;

    2015-01-01

    Background: Spinal fusion is indicated in the surgical management of various spinal disorders. To ensure stabile fusion, bone graft materials are essential. Traditionally allo- or autograft has been used, but both are associated with limitations. Synthetic bone graft materials that reassemble tod...

  3. Going Below Minimums: The Efficacy of Display Enhanced/Synthetic Vision Fusion for Go-Around Decisions during Non-Normal Operations

    Science.gov (United States)

    Prinzel, Lawrence J., III; Kramer, Lynda J.; Bailey, Randall E.

    2007-01-01

    The use of enhanced vision systems in civil aircraft is projected to increase rapidly as the Federal Aviation Administration recently changed the aircraft operating rules under Part 91, revising the flight visibility requirements for conducting approach and landing operations. Operators conducting straight-in instrument approach procedures may now operate below the published approach minimums when using an approved enhanced flight vision system that shows the required visual references on the pilot's Head-Up Display. An experiment was conducted to evaluate the complementary use of synthetic vision systems and enhanced vision system technologies, focusing on new techniques for integration and/or fusion of synthetic and enhanced vision technologies and crew resource management while operating under these newly adopted rules. Experimental results specific to flight crew response to non-normal events using the fused synthetic/enhanced vision system are presented.

  4. Hot fusion, cold fusion

    International Nuclear Information System (INIS)

    The publication of observations of nuclear fusion reactions in electrolysis experiments has led to hope that an easy way to domesticate this major source of energy had been found. In this article are recalled the classical solutions which are studied for hot fusion, the state of the art the difficulties and the perspectives, followed by the present situation concerning the experiments related to what has been called, perhaps a little too quickly, cold fusion

  5. The self-consistent analysis for sub-barrier fusion enhancement effect in Ca + Ca and Ni + Ni

    CERN Document Server

    Ma, N R; Lin, C J; Yang, L; Xu, X X; Sun, L J; Yang, F; Wu, Z D; Zhang, H Q; Liu, Z H; Wang, D X

    2016-01-01

    The fusion dynamic mechanism of heavy-ions at energies near the Coulomb barrier is complicated and still not very clear up to now. Accordingly, a self-consistent method based on the CCFULL calculations has been developed and applied for an ingoing study of the effect of the positive Q-value neutron transfer (PQNT) channels in this work. The typical experimental fusion data of Ca + Ca and Ni + Ni is analyzed within the unified calculation scheme. The PQNT effect in near-barrier fusion is further confirmed based on the self-consistent analysis and extracted quantitatively.

  6. Enhancement of Tropical Land Cover Mapping with Wavelet-Based Fusion and Unsupervised Clustering of SAR and Landsat Image Data

    Science.gov (United States)

    LeMoigne, Jacqueline; Laporte, Nadine; Netanyahuy, Nathan S.; Zukor, Dorothy (Technical Monitor)

    2001-01-01

    The characterization and the mapping of land cover/land use of forest areas, such as the Central African rainforest, is a very complex task. This complexity is mainly due to the extent of such areas and, as a consequence, to the lack of full and continuous cloud-free coverage of those large regions by one single remote sensing instrument, In order to provide improved vegetation maps of Central Africa and to develop forest monitoring techniques for applications at the local and regional scales, we propose to utilize multi-sensor remote sensing observations coupled with in-situ data. Fusion and clustering of multi-sensor data are the first steps towards the development of such a forest monitoring system. In this paper, we will describe some preliminary experiments involving the fusion of SAR and Landsat image data of the Lope Reserve in Gabon. Similarly to previous fusion studies, our fusion method is wavelet-based. The fusion provides a new image data set which contains more detailed texture features and preserves the large homogeneous regions that are observed by the Thematic Mapper sensor. The fusion step is followed by unsupervised clustering and provides a vegetation map of the area.

  7. Bone Marrow Mesenchymal Stem Cells Expressing Baculovirus-Engineered Bone Morphogenetic Protein-7 Enhance Rabbit Posterolateral Fusion.

    Science.gov (United States)

    Liao, Jen-Chung

    2016-01-01

    Previous studies have suggested that bone marrow-derived mesenchymal stem cells (BMDMSCs) genetically modified with baculoviral bone morphogenetic protein-2 (Bac-BMP-2) vectors could achieve successful fusion in a femur defect model or in a spinal fusion model. In this study, BMDMSCs expressing BMP-7 (Bac-BMP-7-BMDMSCs) were generated. We hypothesized that Bac-BMP-7-BMDMSCs could secrete more BMP-7 than untransduced BMDMSCs in vitro and achieve spinal posterolateral fusion in a rabbit model. Eighteen rabbits underwent posterolateral fusion at L4-5. Group I (n = 6) was implanted with collagen-β-tricalcium phosphate (TCP)-hydroxyapatite (HA), Group II (n = 6) was implanted with collagen-β-TCP-HA plus BMDMSCs, and Group III (n = 6) was implanted with collagen-β-TCP-HA plus Bac-BMP-7-BMDMSCs. In vitro production of BMP-7 was quantified with an enzyme-linked immunosorbent assay (ELISA). Spinal fusion was examined using computed tomography (CT), manual palpation, and histological analysis. ELISA demonstrated that Bac-BMP-7-BMDMSCs produced four-fold to five-fold more BMP-7 than did BMDMSCs. In the CT results, 6 fused segments were observed in Group I (50%, 6/12), 8 in Group II (67%, 8/12), and 12 in Group III (100%, 12/12). The fusion rate, determined by manual palpation, was 0% (0/6) in Group I, 0% (0/6) in Group II, and 83% (5/6) in Group III. Histology showed that Group III had more new bone and matured marrow formation. In conclusion, BMDMSCs genetically transduced with the Bac-BMP-7 vector could express more BMP-7 than untransduced BMDMSCs. These Bac-BMP-7-BMDMSCs on collagen-β-TCP-HA scaffolds were able to induce successful spinal fusion in rabbits. PMID:27399674

  8. Bone Marrow Mesenchymal Stem Cells Expressing Baculovirus-Engineered Bone Morphogenetic Protein-7 Enhance Rabbit Posterolateral Fusion

    Directory of Open Access Journals (Sweden)

    Jen-Chung Liao

    2016-07-01

    Full Text Available Previous studies have suggested that bone marrow-derived mesenchymal stem cells (BMDMSCs genetically modified with baculoviral bone morphogenetic protein-2 (Bac-BMP-2 vectors could achieve successful fusion in a femur defect model or in a spinal fusion model. In this study, BMDMSCs expressing BMP-7 (Bac-BMP-7-BMDMSCs were generated. We hypothesized that Bac-BMP-7-BMDMSCs could secrete more BMP-7 than untransduced BMDMSCs in vitro and achieve spinal posterolateral fusion in a rabbit model. Eighteen rabbits underwent posterolateral fusion at L4-5. Group I (n = 6 was implanted with collagen-β-tricalcium phosphate (TCP-hydroxyapatite (HA, Group II (n = 6 was implanted with collagen-β-TCP-HA plus BMDMSCs, and Group III (n = 6 was implanted with collagen-β-TCP-HA plus Bac-BMP-7-BMDMSCs. In vitro production of BMP-7 was quantified with an enzyme-linked immunosorbent assay (ELISA. Spinal fusion was examined using computed tomography (CT, manual palpation, and histological analysis. ELISA demonstrated that Bac-BMP-7-BMDMSCs produced four-fold to five-fold more BMP-7 than did BMDMSCs. In the CT results, 6 fused segments were observed in Group I (50%, 6/12, 8 in Group II (67%, 8/12, and 12 in Group III (100%, 12/12. The fusion rate, determined by manual palpation, was 0% (0/6 in Group I, 0% (0/6 in Group II, and 83% (5/6 in Group III. Histology showed that Group III had more new bone and matured marrow formation. In conclusion, BMDMSCs genetically transduced with the Bac-BMP-7 vector could express more BMP-7 than untransduced BMDMSCs. These Bac-BMP-7-BMDMSCs on collagen-β-TCP-HA scaffolds were able to induce successful spinal fusion in rabbits.

  9. Fusion proteins of HIV-1 envelope glycoprotein gp120 with CD4-induced antibodies showed enhanced binding to CD4 and CD4 binding site antibodies

    International Nuclear Information System (INIS)

    Highlights: ► Some recombinant HIV-1 gp120s do not preserve their conformations on gp140s. ► We hypothesize that CD4i antibodies could induce conformational changes in gp120. ► CD4i antibodies enhance binding of CD4 and CD4bs antibodies to gp120. ► CD4i antibody-gp120 fusion proteins could have potential as vaccine immunogens. -- Abstract: Development of successful AIDS vaccine immunogens continues to be a major challenge. One of the mechanisms by which HIV-1 evades antibody-mediated neutralizing responses is the remarkable conformational flexibility of its envelope glycoprotein (Env) gp120. Some recombinant gp120s do not preserve their conformations on gp140s and functional viral spikes, and exhibit decreased recognition by CD4 and neutralizing antibodies. CD4 binding induces conformational changes in gp120 leading to exposure of the coreceptor-binding site (CoRbs). In this study, we test our hypothesis that CD4-induced (CD4i) antibodies, which target the CoRbs, could also induce conformational changes in gp120 leading to better exposed conserved neutralizing antibody epitopes including the CD4-binding site (CD4bs). We found that a mixture of CD4i antibodies with gp120 only weakly enhanced CD4 binding. However, such interactions in single-chain fusion proteins resulted in gp120 conformations which bound to CD4 and CD4bs antibodies better than the original or mutagenically stabilized gp120s. Moreover, the two molecules in the fusion proteins synergized with each other in neutralizing HIV-1. Therefore, fusion proteins of gp120 with CD4i antibodies could have potential as components of HIV-1 vaccines and inhibitors of HIV-1 entry, and could be used as reagents to explore the conformational flexibility of gp120 and mechanisms of entry and immune evasion.

  10. Fusion of a Short HA2-Derived Peptide Sequence to Cell-Penetrating Peptides Improves Cytosolic Uptake, but Enhances Cytotoxic Activity

    Directory of Open Access Journals (Sweden)

    Igor Kitanovic

    2009-09-01

    Full Text Available Cell-penetrating peptides (CPP have become a widely used tool for efficient cargo delivery into cells. However, one limiting fact is their uptake by endocytosis causing the enclosure of the CPP-cargo construct within endosomes. One often used method to enhance the outflow into the cytosol is the fusion of endosome-disruptive peptide or protein sequences to CPP. But, until now, no studies exist investigating the effects of the fusion peptide to the cellular distribution, structural arrangements and cytotoxic behaviour of the CPP. In this study, we attached a short modified sequence of hemagglutinin subunit HA2 to different CPP and analysed the biologic activity of the new designed peptides. Interestingly, we observed an increased cytosolic distribution but also highly toxic activities in the micromolar range against several cell lines. Structural analysis revealed that attachment of the fusion peptide had profound implications on the whole conformation of the peptide, which might be responsible for membrane interaction and endosome disruption.

  11. Enhancing the production of Fc fusion protein in fed-batch fermentation of Pichia pastoris by design of experiments.

    Science.gov (United States)

    Lin, Henry; Kim, Tina; Xiong, Fei; Yang, Xiaoming

    2007-01-01

    This study focuses on the feasibility of producing a therapeutic Fc fusion protein in Pichia pastoris (P. pastoris) and presents an optimization design of experiment (DOE) strategy in a well-defined experimental space. The parameters examined in this study include pH, temperature, salt supplementation, and batch glycerol concentration. The effects of these process conditions were captured by statistical analysis focusing on growth rate and titer responses. Batch medium and fermentation conditions were also investigated prior to the DOE study in order to provide a favorable condition to enable the production of this Fc fusion protein. The results showed that approximately 373 mg/L of the Fc fusion protein could be produced. The pH was found to be particularly critical for the production of this Fc fusion protein. It was significantly higher than the conventional, recommended pH for P. pastoris fermentation. The development of this process shows that protein production in P. pastoris is protein specific, and there is not a set of pre-defined conditions that can work well for all types of proteins. Thorough process development would need to be performed for every type of protein in order for large-scale production in P. pastoris to be feasible. PMID:17461547

  12. Liposome-enhanced transformation of Streptococcus lactis and plasmid transfer by intergeneric protoplast fusion of Streptococcus lactis and Bacillus subtilis

    NARCIS (Netherlands)

    Vossen, Jos M.B.M. van der; Kok, Jan; Lelie, Daniel van der; Venema, Gerhardus

    1988-01-01

    An efficient protoplast transformation system and a procedure of plasmid transfer by means of protoplast fusion is described for Streptococcus lactis. Protoplasts of S. lactis IL1403 and S. lactis MG1363 were transformed by pGK12 [2.9 MDa erythromycin resistance (Emr)] with an efficiency of 3 × 10^5

  13. Fusion of Hsp70 to Mage-a1 enhances the potency of vaccine-specific immune responses

    OpenAIRE

    Jiang, Juhong; Xie, Dan; Zhang, WenMin; Xiao, Gang; Wen, Jianming

    2013-01-01

    Background Heat shock proteins (HSPs) are capable of promoting antigen presentation of chaperoned peptides through interactions with receptors on antigen presenting cells. This property of HSPs suggests a potential function as an adjuvant-free carrier to stimulate immune responses against a covalently linked fusion partner. MAGE-A1 is a likely candidate for tumor immunotherapy due to its abundant immunogenic epitopes and strict tumor specificity. To analyze the influence of HSP70 conjugation ...

  14. EMP Fusion

    OpenAIRE

    KUNTAY, Isık

    2010-01-01

    This paper introduces a novel fusion scheme, called EMP Fusion, which has the promise of achieving breakeven and realizing commercial fusion power. The method is based on harnessing the power of an electromagnetic pulse generated by the now well-developed flux compression technology. The electromagnetic pulse acts as a means of both heating up the plasma and confining the plasma, eliminating intermediate steps. The EMP Fusion device is simpler compared to other fusion devices and this reduces...

  15. A Novel Long-Acting Human Growth Hormone Fusion Protein (VRS-317): Enhanced In Vivo Potency and Half-Life

    OpenAIRE

    Cleland, Jeffrey L; Geething, Nathan C; Moore, Jerome A; Rogers, Brian C; Spink, Benjamin J; Wang, Chai-Wei; Alters, Susan E.; Stemmer, WIllem P.C.; Schellenberger, Volker

    2012-01-01

    A novel recombinant human growth hormone (rhGH) fusion protein (VRS-317) was designed to minimize receptor-mediated clearance through a reduction in receptor binding without mutations to rhGH by genetically fusing with XTEN amino acid sequences to the N-terminus and the C-terminus of the native hGH sequence. Although in vitro potency of VRS-317 was reduced approximately 12-fold compared with rhGH, in vivo potency was increased because of the greatly prolonged exposure to the target tissues an...

  16. DNA fusion gene vaccines

    DEFF Research Database (Denmark)

    Holst, Peter Johannes; Bassi, Maria Rosaria; Thomsen, Allan Randrup;

    2010-01-01

    DNA vaccines are versatile and safe, but limited immunogenicity has prevented their use in the clinical setting. Experimentally, immunogenicity may be enhanced by the use of new delivery technologies, by coadministration of cytokines and pathogen-associated molecular patterns, or by fusion...... with these modifications, it is likely that the primary use of DNA vaccines may be as primers for viral-vectored vaccines, rather than as single agents. This review discusses the approaches used to enhance DNA vaccine immunogenicity, with a primary focus on fusion strategies that enhance antigen presentation....

  17. Stau-catalyzed Nuclear Fusion

    OpenAIRE

    Hamaguchi, K.; Hatsuda, T.(Theoretical Research Division, Nishina Center, RIKEN, Saitama, 351-0198, Japan); Yanagida, T. T.

    2006-01-01

    We point out that the stau may play a role of a catalyst for nuclear fusions if the stau is a long-lived particle as in the scenario of gravitino dark matter. In this letter, we consider d d fusion under the influence of stau where the fusion is enhanced because of a short distance between the two deuterons. We find that one chain of the d d fusion may release an energy of O(10) GeV per stau. We discuss problems of making the stau-catalyzed nuclear fusion of practical use with the present tec...

  18. Thermal Resonance Fusion

    CERN Document Server

    Dong, Bao-Guo

    2015-01-01

    We first show a possible mechanism to create a new type of nuclear fusion, thermal resonance fusion, i.e. low energy nuclear fusion with thermal resonance of light nuclei or atoms, such as deuterium or tritium. The fusion of two light nuclei has to overcome the Coulomb barrier between these two nuclei to reach up to the interacting region of nuclear force. We found nuclear fusion could be realized with thermal vibrations of crystal lattice atoms coupling with light atoms at low energy by resonance to overcome this Coulomb barrier. Thermal resonances combining with tunnel effects can greatly enhance the probability of the deuterium fusion to the detectable level. Our low energy nuclear fusion mechanism research - thermal resonance fusion mechanism results demonstrate how these light nuclei or atoms, such as deuterium, can be fused in the crystal of metal, such as Ni or alloy, with synthetic thermal vibrations and resonances at different modes and energies experimentally. The probability of tunnel effect at dif...

  19. Fusion of the mouse IgG1 Fc domain to the VHH fragment (ARP1) enhances protection in a mouse model of rotavirus.

    Science.gov (United States)

    Günaydın, Gökçe; Yu, Shengze; Gräslund, Torbjörn; Hammarström, Lennart; Marcotte, Harold

    2016-01-01

    A variable fragment of a heavy chain antibody (VHH) directed against rotavirus, also referred to as anti-rotavirus protein 1 (ARP1), was shown to confer protection against rotavirus induced diarrhea in infant mouse model of rotavirus induced diarrhea. In this study, we have fused the mouse IgG1 Fc to ARP1 to improve the protective capacity of ARP1 by inducing an Fc-mediated effector function. We have shown that the Fc-ARP1 fusion protein confers significantly increased protection against rotavirus in a neonatal mouse model of rotavirus-induced diarrhea by reducing the prevalence, duration and severity of diarrhea and the viral load in the small intestines, suggesting that the Fc part of immunoglobulins may be engaged in Fc-mediated neutralization of rotavirus. Engineered conventional-like antibodies, by fusion of the Fc part of immunoglobulins to antigen-specific heavy-chain only VHH fragments, might be applied to novel antibody-based therapeutic approaches to enhance elimination of pathogens by activation of distinct effector signaling pathways. PMID:27439689

  20. Fusion of the Mycobacterium tuberculosis antigen 85A to an oligomerization domain enhances its immunogenicity in both mice and non-human primates.

    Directory of Open Access Journals (Sweden)

    Alexandra J Spencer

    Full Text Available To prevent important infectious diseases such as tuberculosis, malaria and HIV, vaccines inducing greater T cell responses are required. In this study, we investigated whether fusion of the M. tuberculosis antigen 85A to recently described adjuvant IMX313, a hybrid avian C4bp oligomerization domain, could increase T cell responses in pre-clinical vaccine model species. In mice, the fused antigen 85A showed consistent increases in CD4(+ and CD8(+ T cell responses after DNA and MVA vaccination. In rhesus macaques, higher IFN-γ responses were observed in animals vaccinated with MVA-Ag85A IMX313 after both primary and secondary immunizations. In both animal models, fusion to IMX313 induced a quantitative enhancement in the response without altering its quality: multifunctional cytokines were uniformly increased and differentiation into effector and memory T cell subsets was augmented rather than skewed. An extensive in vivo characterization suggests that IMX313 improves the initiation of immune responses as an increase in antigen 85A specific cells was observed as early as day 3 after vaccination. This report demonstrates that antigen multimerization using IMX313 is a simple and effective cross-species method to improve vaccine immunogenicity with potentially broad applicability.

  1. Boron-proton nuclear-fusion enhancement induced in boron-doped silicon targets by low-contrast pulsed laser

    Czech Academy of Sciences Publication Activity Database

    Picciotto, A.; Margarone, Daniele; Velyhan, Andriy; Bellutti, P.; Krása, Josef; Szydlowsky, A.; Bertuccio, G.; Shi, Y.; Mangione, A.; Prokůpek, Jan; Malinowska, A.; Krouský, Eduard; Ullschmied, Jiří; Láska, Leoš; Kucharik, M.; Korn, Georg

    2014-01-01

    Roč. 4, č. 3 (2014), "031030-1"-"031030-8". ISSN 2160-3308 R&D Projects: GA MŠk ED1.1.00/02.0061; GA MŠk(CZ) LD14089; GA MŠk LM2010014 Grant ostatní: ELI Beamlines(XE) CZ.1.05/1.1.00/02.0061; AVČR(CZ) M100101210; FP7 Laserlab Europe(XE) 284464 Institutional support: RVO:68378271 ; RVO:61389021 Keywords : thermonuclear fusion * fast ions * plasmas * energy * acceleration * hydrogen * detector Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders; BL - Plasma and Gas Discharge Physics (UFP-V) Impact factor: 9.043, year: 2014

  2. Comparative transcriptomic analysis reveals the oncogenic fusion protein PAX3-FOXO1 globally alters mRNA and miRNA to enhance myoblast invasion.

    Science.gov (United States)

    Loupe, J M; Miller, P J; Bonner, B P; Maggi, E C; Vijayaraghavan, J; Crabtree, J S; Taylor, C M; Zabaleta, J; Hollenbach, A D

    2016-01-01

    Rhabdomyosarcoma, one of the most common childhood sarcomas, is comprised of two main subtypes, embryonal and alveolar (ARMS). ARMS, the more aggressive subtype, is primarily characterized by the t(2;13)(p35;p14) chromosomal translocation, which fuses two transcription factors, PAX3 and FOXO1 to generate the oncogenic fusion protein PAX3-FOXO1. Patients with PAX3-FOXO1-postitive tumors have a poor prognosis, in part due to the enhanced local invasive capacity of these cells, which leads to the increased metastatic potential for this tumor. Despite this knowledge, little is known about the role that the oncogenic fusion protein has in this increased invasive potential. In this report we use large-scale comparative transcriptomic analyses in physiologically relevant primary myoblasts to demonstrate that the presence of PAX3-FOXO1 is sufficient to alter the expression of 70 mRNA and 27 miRNA in a manner predicted to promote cellular invasion. In contrast the expression of PAX3 alters 60 mRNA and 23 miRNA in a manner predicted to inhibit invasion. We demonstrate that these alterations in mRNA and miRNA translate into changes in the invasive potential of primary myoblasts with PAX3-FOXO1 increasing invasion nearly 2-fold while PAX3 decreases invasion nearly 4-fold. Taken together, these results allow us to build off of previous reports and develop a more expansive molecular model by which the presence of PAX3-FOXO1 alters global gene regulatory networks to enhance the local invasiveness of cells. Further, the global nature of our observed changes highlights the fact that instead of focusing on a single-gene target, we must develop multi-faceted treatment regimens targeting multiple genes of a single oncogenic phenotype or multiple genes that target different oncogenic phenotypes for tumor progression. PMID:27454080

  3. Fusion rings and fusion ideals

    DEFF Research Database (Denmark)

    Andersen, Troels Bak

    This dissertation investigates fusion rings, which are Grothendieck groups of rigid, monoidal, semisimple, abelian categories. Special interest is in rational fusion rings, i.e., fusion rings which admit a finite basis, for as commutative rings they may be presented as quotients of polynomial rings...... by the so-called fusion ideals. The fusion rings of Wess-Zumino-Witten models have been widely studied and are well understood in terms of precise combinatorial descriptions and explicit generating sets of the fusion ideals. They also appear in another, more general, setting via tilting modules for quantum...

  4. Fusion Canada

    International Nuclear Information System (INIS)

    This first issue of a quarterly newsletter announces the startup of the Tokamak de Varennes, describes Canada's national fusion program, and outlines the Canadian Fusion Fuels Technology Program. A map gives the location of the eleven principal fusion centres in Canada. (L.L.)

  5. Membrane fusion

    DEFF Research Database (Denmark)

    Bendix, Pól Martin

    2015-01-01

    At Stanford University, Boxer lab, I worked on membrane fusion of small unilamellar lipid vesicles to flat membranes tethered to glass surfaces. This geometry closely resembles biological systems in which liposomes fuse to plasma membranes. The fusion mechanism was studied using DNA zippering...... between complementary strands linked to the two apposing membranes closely mimicking the zippering mechanism of SNARE fusion complexes....

  6. A novel long-acting human growth hormone fusion protein (VRS-317): enhanced in vivo potency and half-life.

    Science.gov (United States)

    Cleland, Jeffrey L; Geething, Nathan C; Moore, Jerome A; Rogers, Brian C; Spink, Benjamin J; Wang, Chai-Wei; Alters, Susan E; Stemmer, Willem P C; Schellenberger, Volker

    2012-08-01

    A novel recombinant human growth hormone (rhGH) fusion protein (VRS-317) was designed to minimize receptor-mediated clearance through a reduction in receptor binding without mutations to rhGH by genetically fusing with XTEN amino acid sequences to the N-terminus and the C-terminus of the native hGH sequence. Although in vitro potency of VRS-317 was reduced approximately 12-fold compared with rhGH, in vivo potency was increased because of the greatly prolonged exposure to the target tissues and organs. VRS-317 was threefold more potent than daily rhGH in hypophysectomized rats and fivefold more potent than daily rhGH in juvenile monkeys. In juvenile monkeys, a monthly dose of 1.4 mg/kg VRS-317 (equivalent to 0.26 mg/kg rhGH) caused a sustained pharmacodynamic response for 1 month equivalent to 0.05 mg/kg/day rhGH (1.4 mg/kg rhGH total over 28 days). In monkeys, VRS-317, having a terminal elimination half-life of approximately 110 h, was rapidly and near-completely absorbed, and was well tolerated with no observed adverse effects after every alternate week subcutaneous dosing for 14 weeks. VRS-317 also did not cause lipoatrophy in pig and monkey studies. VRS-317 is currently being studied in GH-deficient patients to confirm the observations in these animal studies. PMID:22678811

  7. Enhancement of the power system efficiency using the hybrid-type harmonic filters for a KSTAR nuclear fusion experimental system

    International Nuclear Information System (INIS)

    Highlights: → The low power factor and power quality problems are occurred by the operation of the PF facility in KSTAR system. We model the power system of KSTAR system including the PF facility. We show a method of the filter insertion to improve the problem and conduct the simulations to verify our method. - Abstract: The KSTAR system, which includes a large amount of nonlinear load, is a relatively high reactive power consumption load which injects harmonic currents into the power system which could result in the possibility for a power system perturbation to occur in the transmission lines, affecting nearby customers. In order to maintain the power quality and power factor in the inner system of the KSTAR system and the adjacent distribution lines, the assessment of the effect of the DC power supply in the KSTAR system is required for appropriate countermeasures to be put in place. In this paper, on the basis of a preliminary inspection of the power system near a KSTAR system, the simulation of a compensating device is performed for the prevention of abnormal voltage variations caused by a large amount of reactive and nonlinear load. In addition, through the comparison of the pre- and post-application of compensation devices in the actual power system, it is verified that a stable operation of the KSTAR nuclear fusion experimental system can be achieved.

  8. Over-expression of BvMTSH, a fusion gene for maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase, enhances drought tolerance in transgenic rice.

    Science.gov (United States)

    Joo, Joungsu; Choi, Hae Jong; Lee, Youn Hab; Lee, Sarah; Lee, Choong Hwan; Kim, Chung Ho; Cheong, Jong-Joo; Choi, Yang Do; Song, Sang Ik

    2014-01-01

    Plant abiotic stress tolerance has been modulated by engineering the trehalose synthesis pathway. However, many stress-tolerant plants that have been genetically engineered for the trehalose synthesis pathway also show abnormal development. The metabolic intermediate trehalose 6-phosphate has the potential to cause aberrations in growth. To avoid growth inhibition by trehalose 6-phosphate, we used a gene that encodes a bifunctional in-frame fusion (BvMTSH) of maltooligosyltrehalose synthase (BvMTS) and maltooligosyltrehalose trehalohydrolase (BvMTH) from the nonpathogenic bacterium Brevibacterium helvolum. BvMTS converts maltooligosaccharides into maltooligosyltrehalose and BvMTH releases trehalose. Transgenic rice plants that over-express BvMTSH under the control of the constitutive rice cytochrome c promoter (101MTSH) or the ABA-inducible Ai promoter (105MTSH) show enhanced drought tolerance without growth inhibition. Moreover, 101MTSH and 105MTSH showed an ABA-hyposensitive phenotype in the roots. Our results suggest that over-expression of BvMTSH enhances drought-stress tolerance without any abnormal growth and showes ABA hyposensitive phenotype in the roots. PMID:24209631

  9. FN-DFE: Fuzzy-Neural Data Fusion Engine for Enhanced State-Awareness of Resilient Hybrid Energy System

    Energy Technology Data Exchange (ETDEWEB)

    Ondrej Linda; Dumidu Wijayasekara; Milos Manic; Craig Rieger

    2014-11-01

    Resiliency and improved state-awareness of modern critical infrastructures, such as energy production and industrial systems, is becoming increasingly important. As control systems become increasingly complex, the number of inputs and outputs increase. Therefore, in order to maintain sufficient levels of state-awareness, a robust system state monitoring must be implemented that correctly identifies system behavior even when one or more sensors are faulty. Furthermore, as intelligent cyber adversaries become more capable, incorrect values may be fed to the operators. To address these needs, this paper proposes a Fuzzy-Neural Data Fusion Engine (FN-DFE) for resilient state-awareness of control systems. The designed FN-DFE is composed of a three-layered system consisting of: 1) traditional threshold based alarms, 2) anomalous behavior detector using self-organizing fuzzy logic system, and 3) artificial neural network based system modeling and prediction. The improved control system state-awareness is achieved via fusing input data from multiple sources and combining them into robust anomaly indicators. In addition, the neural network based signal predictions are used to augment the resiliency of the system and provide coherent state-awareness despite temporary unavailability of sensory data. The proposed system was integrated and tested with a model of the Idaho National Laboratory’s (INL) hybrid energy system facility know as HYTEST. Experimental results demonstrate that the proposed FN-DFE provides timely plant performance monitoring and anomaly detection capabilities. It was shown that the system is capable of identifying intrusive behavior significantly earlier than conventional threshold based alarm systems.

  10. Fusion breeder

    International Nuclear Information System (INIS)

    The fusion breeder is a fusion reactor designed with special blankets to maximize the transmutation by 14 MeV neutrons of uranium-238 to plutonium or thorium to uranium-233 for use as a fuel for fission reactors. Breeding fissile fuels has not been a goal of the US fusion energy program. This paper suggests it is time for a policy change to make the fusion breeder a goal of the US fusion program and the US nuclear energy program. The purpose of this paper is to suggest this policy change be made and tell why it should be made, and to outline specific research and development goals so that the fusion breeder will be developed in time to meet fissile fuel needs

  11. Controlled fusion

    International Nuclear Information System (INIS)

    During the last fifty years the researches on controlled thermonuclear fusion reached great performance in the magnetic confinement (tokamaks) as in the inertial confinement (lasers). But the state of the art is not in favor of the apparition of the fusion in the energy market before the second half of the 21 century. To explain this opinion the author presents the fusion reactions of light nuclei and the problems bound to the magnetic confinement. (A.L.B.)

  12. Fusion Machinery

    DEFF Research Database (Denmark)

    Sørensen, Jakob Balslev; Milosevic, Ira

    2015-01-01

    SNARE proteins constitute the minimal machinery needed for membrane fusion. SNAREs operate by forming a complex, which pulls the lipid bilayers into close contact and provides the mechanical force needed for lipid bilayer fusion. At the chemical synapse, SNARE-complex formation between the vesicu......SNARE proteins constitute the minimal machinery needed for membrane fusion. SNAREs operate by forming a complex, which pulls the lipid bilayers into close contact and provides the mechanical force needed for lipid bilayer fusion. At the chemical synapse, SNARE-complex formation between...

  13. Fusion Implementation

    International Nuclear Information System (INIS)

    If a fusion DEMO reactor can be brought into operation during the first half of this century, fusion power production can have a significant impact on carbon dioxide production during the latter half of the century. An assessment of fusion implementation scenarios shows that the resource demands and waste production associated with these scenarios are manageable factors. If fusion is implemented during the latter half of this century it will be one element of a portfolio of (hopefully) carbon dioxide limiting sources of electrical power. It is time to assess the regional implications of fusion power implementation. An important attribute of fusion power is the wide range of possible regions of the country, or countries in the world, where power plants can be located. Unlike most renewable energy options, fusion energy will function within a local distribution system and not require costly, and difficult, long distance transmission systems. For example, the East Coast of the United States is a prime candidate for fusion power deployment by virtue of its distance from renewable energy sources. As fossil fuels become less and less available as an energy option, the transmission of energy across bodies of water will become very expensive. On a global scale, fusion power will be particularly attractive for regions separated from sources of renewable energy by oceans

  14. Enhanced immunogenicity of pneumococcal surface adhesin A (PsaA in mice via fusion to recombinant human B lymphocyte stimulator (BLyS

    Directory of Open Access Journals (Sweden)

    Mambula Salamatu S

    2011-02-01

    Full Text Available Abstract Background B lymphocyte stimulator (BLyS is a member of the tumor necrosis factor superfamily of ligands that mediates its action through three known receptors. BLyS has been shown to enhance the production of antibodies against heterologous antigens when present at elevated concentrations, supporting an immunostimulatory role for BLyS in vivo. Methods We constructed a fusion protein consisting of human BLyS and Pneumococcal Surface Adhesin A (PsaA and used this molecule to immunize mice. The immunostimulatory attributes mediated by BLyS in vivo were evaluated by characterizing immune responses directed against PsaA. Results The PsaA-BLyS fusion protein was able to act as a co-stimulant for murine spleen cell proliferation induced with F(ab'2 fragments of anti-IgM in vitro in a fashion similar to recombinant BLyS, and immunization of mice with the PsaA-BLyS fusion protein resulted in dramatically elevated serum antibodies specific for PsaA. Mice immunized with PsaA admixed with recombinant BLyS exhibited only modest elevations in PsaA-specific responses following two immunizations, while mice immunized twice with PsaA alone exhibited undetectable PsaA-specific serum antibody responses. Sera obtained from PsaA-BLyS immunized mice exhibited high titers of IgG1, IgG2a, IgG2b, and IgG3, but no IgA, while mice immunized with PsaA admixed with BLyS exhibited only elevated titers of IgG1 following two immunizations. Splenocytes from PsaA-BLyS immunized mice exhibited elevated levels of secretion of IL-2, IL-4 and IL-5, and a very modest but consistent elevation of IFN-γ following in vitro stimulation with PsaA. In contrast, mice immunized with either PsaA admixed with BLyS or PsaA alone exhibited modestly elevated to absent PsaA-specific recall responses for the same cytokines. Mice deficient for one of the three receptors for BLyS designated Transmembrane activator, calcium modulator, and cyclophilin ligand [CAML] interactor (TACI exhibited

  15. Combined virus-like particle and fusion protein-encoding DNA vaccination of cotton rats induces protection against respiratory syncytial virus without causing vaccine-enhanced disease.

    Science.gov (United States)

    Hwang, Hye Suk; Lee, Young-Tae; Kim, Ki-Hye; Park, Soojin; Kwon, Young-Man; Lee, Youri; Ko, Eun-Ju; Jung, Yu-Jin; Lee, Jong Seok; Kim, Yu-Jin; Lee, Yu-Na; Kim, Min-Chul; Cho, Minkyoung; Kang, Sang-Moo

    2016-07-01

    A safe and effective vaccine against respiratory syncytial virus (RSV) should confer protection without causing vaccine-enhanced disease. Here, using a cotton rat model, we investigated the protective efficacy and safety of an RSV combination vaccine composed of F-encoding plasmid DNA and virus-like particles containing RSV fusion (F) and attachment (G) glycoproteins (FFG-VLP). Cotton rats with FFG-VLP vaccination controlled lung viral replication below the detection limit, and effectively induced neutralizing activity and antibody-secreting cell responses. In comparison with formalin inactivated RSV (FI-RSV) causing severe RSV disease after challenge, FFG-VLP vaccination did not cause weight loss, airway hyper-responsiveness, IL-4 cytokines, histopathology, and infiltrates of proinflammatory cells such as eosinophils. FFG-VLP was even more effective in preventing RSV-induced pulmonary inflammation than live RSV infections. This study provides evidence that FFG-VLP can be developed into a safe and effective RSV vaccine candidate. PMID:27123586

  16. Image fusion

    Science.gov (United States)

    Pavel, M.

    1993-01-01

    The topics covered include the following: a system overview of the basic components of a system designed to improve the ability of a pilot to fly through low-visibility conditions such as fog; the role of visual sciences; fusion issues; sensor characterization; sources of information; image processing; and image fusion.

  17. Sex-biased transcription enhancement by a 5' tethered Gal4-MOF histone acetyltransferase fusion protein in Drosophila

    Directory of Open Access Journals (Sweden)

    Belikoff Esther J

    2010-11-01

    Full Text Available Abstract Background In male Drosophila melanogaster, the male specific lethal (MSL complex is somehow responsible for a two-fold increase in transcription of most X-linked genes, which are enriched for histone H4 acetylated at lysine 16 (H4K16ac. This acetylation requires MOF, a histone acetyltransferase that is a component of the MSL complex. MOF also associates with the non-specific lethal or NSL complex. The MSL complex is bound within active genes on the male X chromosome with a 3' bias. In contrast, the NSL complex is enriched at promoter regions of many autosomal and X-linked genes in both sexes. In this study we have investigated the role of MOF as a transcriptional activator. Results MOF was fused to the DNA binding domain of Gal4 and targeted to the promoter region of UAS-reporter genes in Drosophila. We found that expression of a UAS-red fluorescent protein (DsRed reporter gene was strongly induced by Gal4-MOF. However, DsRed RNA levels were about seven times higher in female than male larvae. Immunostaining of polytene chromosomes showed that Gal4-MOF co-localized with MSL1 to many sites on the X chromosome in male but not female nuclei. However, in female nuclei that express MSL2, Gal4-MOF co-localized with MSL1 to many sites on polytene chromosomes but DsRed expression was reduced. Mutation of conserved active site residues in MOF (Glu714 and Cys680 reduced HAT activity in vitro and UAS-DsRed activation in Drosophila. In the presence of Gal4-MOF, H4K16ac levels were enriched over UAS-lacZ and UAS-arm-lacZ reporter genes. The latter utilizes the constitutive promoter from the arm gene to drive lacZ expression. In contrast to the strong induction of UAS-DsRed expression, UAS-arm-lacZ expression increased by about 2-fold in both sexes. Conclusions Targeting MOF to reporter genes led to transcription enhancement and acetylation of histone H4 at lysine 16. Histone acetyltransferase activity was required for the full transcriptional

  18. Enhancing tumor-specific intracellular delivering efficiency of cell-penetrating peptide by fusion with a peptide targeting to EGFR.

    Science.gov (United States)

    Nguyen, Long The; Yang, Xu-Zhong; Du, Xuan; Wang, Jia-Wei; Zhang, Rui; Zhao, Jian; Wang, Fu-Jun; Dong, Yang; Li, Peng-Fei

    2015-05-01

    Cell-penetrating peptides (CPPs) are well known as intracellular delivery vectors. However, unsatisfactory delivery efficiency and poor specificity are challenging barriers to CPP applications at the clinical trial stage. Here, we showed that S3, an EGFR-binding domain derived from vaccinia virus growth factor, when fused to a CPP such as HBD or TAT can substantially enhance its internalization efficiency and tumor selectivity. The uptake of S3-HBD (S3H) recombinant molecule by tumor cells was nearly 80 folds increased compared to HBD alone. By contrast, the uptake of S3H by non-neoplastic cells still remained at a low level. The specific recognition between S3 and its receptor, EGFR, as well as between HBD and heparan sulfate proteoglycans on the cell surface was essential for these improvements, suggesting a syngeneic effect between the two functional domains in conjugation. This syngeneic effect is likely similar to that of the heparin-binding epidermal growth factor, which is highly abundant particularly in metastatic tumors. The process that S3H entered cells was dependent on time, dosage, and energy, via macropinocytosis pathway. With excellent cell-penetrating efficacy and a novel tumor-targeting ability, S3H appears as a promising candidate vector for targeted anti-cancer drug delivery. PMID:25655386

  19. The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain

    Directory of Open Access Journals (Sweden)

    Lucile Tran

    2012-01-01

    Full Text Available Aim. To investigate the ability of recombinant modified vaccinia virus Ankara (rMVA vector to induce an immune response against a well-tolerated self-antigen. Methods. rMVA vectors expressing different form of α-fetoprotein (AFP were produced and characterized. Naïve mice were vaccinated with MVA vectors expressing the AFP antigen in either a secreted, or a membrane-bound, or an intracellular form. The immune response was monitored by an IFNΓ ELISpot assay and antibody detection. Results. Vaccination with the membrane-associated form of AFP induced a stronger CD8+ T-cell response compared to the ones obtained with the MVA encoding the secreted or the intracellular forms of AFP. Moreover, the vaccination with the membrane-bound AFP elicited the production of AFP-specific antibodies. Conclusions. The AFP transmembrane form is more immunogenic. Expressing a membrane-bound form in the context of an MVA vaccination could enhance the immunogenicity of a self-antigen.

  20. Magnetically Catalyzed Fusion

    OpenAIRE

    Heyl, Jeremy S.; Hernquist, Lars

    1996-01-01

    We calculate the reaction cross-sections for the fusion of hydrogen and deuterium in strong magnetic fields as are believed to exist in the atmospheres of neutron stars. We find that in the presence of a strong magnetic field ($B \\gsim 10^{12}$G), the reaction rates are many orders of magnitude higher than in the unmagnetized case. The fusion of both protons and deuterons are important over a neutron star's lifetime for ultrastrong magnetic fields ($B \\sim 10^{16}$G). The enhancement may have...

  1. Sonic Hedgehog Ligand Partners with Caveolin-1 for Intracellular Transportation

    OpenAIRE

    Mao, Hua; Diehl, Anna Mae; Li, Yin-Xiong

    2009-01-01

    Prenatal alcohol exposure is the most common environmental factor leading to congenital birth defects in the United States. Although significant progress has been made in this field, the detailed molecular pathology of Fetal Alcohol Syndrome (FAS) remains to be determined. Previously, we have shown that alcohol exposure perturbs Hedgehog signal transduction in zebrafish embryos by inhibiting the post-translational cholesterol modification of Sonic hedgehog (Shh), leading to decreased levels o...

  2. Peaceful fusion

    International Nuclear Information System (INIS)

    Like other intense neutron sources fusion reactors have in principle a potential to be used for military purposes. Although the use of fissile material is usually not considered when thinking of fusion reactors (except in fusion-fission hybrid concepts) quantitative estimates about the possible production potential of future commercial fusion reactor concepts show that significant amounts of weapon grade fissile materials could be produced even with very limited amounts of source materials. In this talk detailed burnup calculations with VESTA and MCMATH using an MCNP model of the PPCS-A will be presented. We compare different irradiation positions and the isotopic vectors of the plutonium bred in different blankets of the reactor wall with the liquid lead-lithium alloy replaced by uranium. The technical, regulatory and policy challenges to manage the proliferation risks of fusion power will be addressed as well. Some of these challenges would benefit if addressed at an early stage of the research and development process. Hence, research on fusion reactor safeguards should start as early as possible and accompany the current research on experimental fusion reactors.

  3. Magneized target fusion: An overview of the concept

    International Nuclear Information System (INIS)

    Magnetized target fusion (MTF) seeks to take advantage of the reduction of thermal conductivity through the application of a strong magneticfield and thereby ease the requirements for reaching fusion conditions in a thermonuclear (TN) fusion fuel. A potentially important benefit of the strong field in the partial trapping of energetic charged particles to enhance energy deposition by the TN fusion reaction products. The essential physics is described. MTF appears to lead to fusion targets that require orders of magnitude less power and intensity for fusion ignition than currently proposed (unmagnetized) inertial confinement fusion (ICF) targets do, making some very energetic pulsed power drivers attractive for realizing controlled fusion

  4. Cold fusion

    International Nuclear Information System (INIS)

    So called 'cold fusion phenomena' are not confirmed yet. Excess heat generation is very delicate one. Neutron generation is most reliable results, however, the records are erratic and the same results could not be repeated. So there is no reason to exclude the malfunction of testing instruments. The same arguments arise in recording 4He, 3He, 3H, which are not rich in quantity basically. An experiment where plenty of 4He were recorded is attached in appendix. The problem is that we are trying to search cold fusion which is permitted by nature or not. The famous tunneling effect in quantum mechanics will answer it, however, the most fusion rate is known to be negligible. The focus of this project is on the theme that how to increase that negligible fusion rate. 6 figs, 4 tabs, 1512 refs. (Author)

  5. Spinal fusion

    Science.gov (United States)

    ... the wound or vertebral bones Damage to a spinal nerve, causing weakness, pain, loss of sensation, problems with your bowels or bladder The vertebrae above and below the fusion are more likely to wear away, leading to more problems later

  6. Cold fusion

    Energy Technology Data Exchange (ETDEWEB)

    Suh, Suk Yong; Sung, Ki Woong; Kang, Joo Sang; Lee, Jong Jik [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1995-02-01

    So called `cold fusion phenomena` are not confirmed yet. Excess heat generation is very delicate one. Neutron generation is most reliable results, however, the records are erratic and the same results could not be repeated. So there is no reason to exclude the malfunction of testing instruments. The same arguments arise in recording {sup 4}He, {sup 3}He, {sup 3}H, which are not rich in quantity basically. An experiment where plenty of {sup 4}He were recorded is attached in appendix. The problem is that we are trying to search cold fusion which is permitted by nature or not. The famous tunneling effect in quantum mechanics will answer it, however, the most fusion rate is known to be negligible. The focus of this project is on the theme that how to increase that negligible fusion rate. 6 figs, 4 tabs, 1512 refs. (Author).

  7. Fusion energy

    International Nuclear Information System (INIS)

    The efforts of the Chemical Technology Division in fusion energy include the areas of fuel handling, processing, and containment. Current studies are concerned largely with the development of vacuum pumps for fusion reactors and experiments and with development and evaluation of techniques for recovering tritium from solid or liquid breeding blankets. In addition, a small effort is devoted to support of the ORNL design of a major Tokamak experiment, The Next Step (TNS)

  8. Sulfated polysaccharide, curdlan sulfate, efficiently prevents entry/fusion and restricts antibody-dependent enhancement of dengue virus infection in vitro: a possible candidate for clinical application.

    Directory of Open Access Journals (Sweden)

    Koji Ichiyama

    Full Text Available Curdlan sulfate (CRDS, a sulfated 1→3-β-D glucan, previously shown to be a potent HIV entry inhibitor, is characterized in this study as a potent inhibitor of the Dengue virus (DENV. CRDS was identified by in silico blind docking studies to exhibit binding potential to the envelope (E protein of the DENV. CRDS was shown to inhibit the DENV replication very efficiently in different cells in vitro. Minimal effective concentration of CRDS was as low as 0.1 µg/mL in LLC-MK2 cells, and toxicity was observed only at concentrations over 10 mg/mL. CRDS can also inhibit DENV-1, 3, and 4 efficiently. CRDS did not inhibit the replication of DENV subgenomic replicon. Time of addition experiments demonstrated that the compound not only inhibited viral infection at the host cell binding step, but also at an early post-attachment step of entry (membrane fusion. The direct binding of CRDS to DENV was suggested by an evident reduction in the viral titers after interaction of the virus with CRDS following an ultrafiltration device separation, as well as after virus adsorption to an alkyl CRDS-coated membrane filter. The electron microscopic features also showed that CRDS interacted directly with the viral envelope, and caused changes to the viral surface. CRDS also potently inhibited DENV infection in DC-SIGN expressing cells as well as the antibody-dependent enhancement of DENV-2 infection. Based on these data, a probable binding model of CRDS to DENV E protein was constructed by a flexible receptor and ligand docking study. The binding site of CRDS was predicted to be at the interface between domains II and III of E protein dimer, which is unique to this compound, and is apparently different from the β-OG binding site. Since CRDS has already been tested in humans without serious side effects, its clinical application can be considered.

  9. Structural materials for fusion magnets

    International Nuclear Information System (INIS)

    Of major technical and cost impact to Magnetic Fusion Energy development are the materials for the magnet structure. Likened to gas pressure, the magnetic field lines try to expand the structure with equivalent pressures up to 1000 atm. Not only are large tensile forces produced, but significant bending forces may also be present. To withstand these forces in the restricted spaces available, materials of exceptional strength and toughness are required. In this regard, the low-temperature environment of superconducting magnets can be an advantage because many materials exhibit enhanced properties at reduced temperatures. Those materials and fabrication techniques that are attractive to fusion magnets are discussed and relative comparisons made. Considerations such as strength, toughness, and joining techniques are balanced agains recommended design criteria to reach an optimum design. Several examples of material selection are cited for large fusion magnets such as Baseball II, the Mirror Fusion Test Facility, the Toroidal Fusion Test Facility, and the Large Coil Project. (orig.)

  10. Excitation of muonic molecules ddμ and dtμ by super-intense attosecond soft X-ray laser pulses: shaped post-laser-pulse muonic oscillations and enhancement of nuclear fusion

    International Nuclear Information System (INIS)

    The quantum dynamics of muonic molecular ions ddμ and dtμ excited by linearly polarized along the molecular (z)-axis super-intense laser pulses is studied beyond the Born–Oppenheimer approximation by the numerical solution of the time-dependent Schroedinger equation within a three-dimensional model, including the internuclear distance R and muon coordinates z and ρ. The peak-intensity of the super-intense laser pulses used in our simulations is I0 = 3.51 × 1022 W/cm2 and the wavelength is λl = 5 nm. In both ddμ and dtμ, expectation values〈z〉 and 〈ρ〉 of muon demonstrate “post-laser-pulse” oscillations after the ends of the laser pulses. In ddμ post-laser-pulse z-oscillations appear as shaped nonoverlapping “echo-pulses”. In dtμ post-laser-pulse muonic z-oscillations appear as comparatively slow large-amplitude oscillations modulated with small-amplitude pulsations. The post-laser-pulse ρ-oscillations in both ddμ and dtμ appear, for the most part, as overlapping “echo-pulses”. The post-laser-pulse oscillations do not occur if the Born–Oppenheimer approximation is employed. Power spectra generated due to muonic motion along both optically active z and optically passive ρ degrees of freedom are calculated. The fusion probability in dtμ can be increased by more than 11 times by making use of three sequential super-intense laser pulses. The energy released from the dt fusion in dtμ can by more than 20 GeV exceed the energy required to produce a usable muon and the energy of the laser pulses used to enhance the fusion. The possibility of power production from the laser-enhanced muon-catalyzed fusion is discussed. (author)

  11. Excitation of muonic molecules ddμ and dtμ by super-intense attosecond soft X-ray laser pulses: Shaped post-laser-pulse muonic oscillations and enhancement of nuclear fusion

    Science.gov (United States)

    Bandrauk, André D.; Paramonov, Guennaddi K.

    2014-07-01

    The quantum dynamics of muonic molecular ions ddμ and dtμ excited by linearly polarized along the molecular (z)-axis super-intense laser pulses is studied beyond the Born-Oppenheimer approximation by the numerical solution of the time-dependent Schrödinger equation within a three-dimensional model, including the internuclear distance R and muon coordinates z and ρ. The peak-intensity of the super-intense laser pulses used in our simulations is I0 = 3.51 × 1022 W/cm2 and the wavelength is λl = 5 nm. In both ddμ and dtμ, expectation values and of muon demonstrate "post-laser-pulse" oscillations after the ends of the laser pulses. In ddμ post-laser-pulse z-oscillations appear as shaped nonoverlapping "echo-pulses". In dtμ post-laser-pulse muonic z-oscillations appear as comparatively slow large-amplitude oscillations modulated with small-amplitude pulsations. The post-laser-pulse ρ-oscillations in both ddμ and dtμ appear, for the most part, as overlapping "echo-pulses". The post-laser-pulse oscillations do not occur if the Born-Oppenheimer approximation is employed. Power spectra generated due to muonic motion along both optically active z and optically passive ρ degrees of freedom are calculated. The fusion probability in dtμ can be increased by more than 11 times by making use of three sequential super-intense laser pulses. The energy released from the dt fusion in dtμ can by more than 20 GeV exceed the energy required to produce a usable muon and the energy of the laser pulses used to enhance the fusion. The possibility of power production from the laser-enhanced muon-catalyzed fusion is discussed.

  12. Down-regulation of Caveolin-l, GFAP, BDNF expression in hippocampus of neonatal rats induced by maternal sepa-ration%新生期母婴分离导致大鼠海马区Caveolin-1、GFAP、BDNF表达下调

    Institute of Scientific and Technical Information of China (English)

    乔力媛; 孙鸿燕; 董文斌; 张建彬; 李清平; 雷小平; 康兰

    2013-01-01

      目的探讨母婴分离导致大鼠成年后行为异常的早期存在机制。方法新生大鼠随机分为分离组和对照组,分离组于出生后第2至21天与母鼠每天分离3 h,对照组不做任何处理。在大鼠出生后第7、14、21天处死,取脑组织,以免疫组化检测大鼠海马组织小窝蛋白-1(Caveolin-1)、胶质纤维酸性蛋白(GFAP)、脑源性神经生长因子(BDNF)。以Image-Pro Plus图像分析系统对免疫组化图像进行半定量分析。结果与对照组相比,分离组大鼠出生后第7天Caveolin-l表达的差异无统计学意义,而GFAP和BDNF表达增加,差异有统计学意义(P<0.05);出生后第14和21天,分离组大鼠Caveolin-l、GFAP、BDNF表达下降,差异均有统计学意义(P<0.05)。结论新生期母婴分离可致大鼠海马Caveolin-l、BDNF、GFAP的表达出现不同程度的下降,这可能与母婴分离致成年后行为异常有关。%Objective To explore the potential mechanism of abnormal behavior resulted from maternal separation in neo-natal period in rat. Methods Neonatal rats were equally and randomly divided into maternal separation group and control group. The rats in maternal separation group were separated from the dam for 3h per day on postnatal days (PND) 2 to 21, nothing was done to the rats in the control group. The brain tissues were taken out after being killed on PND 7, 14, and 21. The expressions of Caveolin-l, BDNF and GFAP in hippocampal formation were detected by immunohistochemistry. Semiquantitative assessment of immunohistochemical images was performed by Image-Pro Plus software. Results Compared with control groups, the expres-sion of Caveolin-l on PND 7 had no signiifcant change, while BDNF and GFAP were signiifcantly increased in maternal separa-tion group (P<0.05). On PND 14 and 21, the expressions of Caveolin-l, BDNF and GFAP were signiifcantly decreased in maternal separation group (P<0.05). Conclusions

  13. Tetanus Toxin Fragment C Expressed in Live Salmonella Vaccines Enhances Antibody Responses to Its Fusion Partner Schistosoma haematobium Glutathione S-Transferase

    OpenAIRE

    Lee, Jeong Jin; Sinha, Katharine A.; Harrison, Julia A.; de Hormaeche, Raquel Demarco; Riveau, Gilles; Pierce, Raymond J.; Capron, Andre; Wilson, R. Alan; Khan, C.M. Anjam

    2000-01-01

    Tetanus toxoid has been used widely as an adjuvant. The atoxic fragment C from tetanus toxin (TetC) is potently immunogenic when expressed in Salmonella vaccine strains and has been used as a fusion partner for antigens (Ag). However, there has been no formal comparison of the immunomodulatory impact of TetC on its fusion partners. In this study, we have addressed this important issue. The protective 28-kDa glutathione S-transferase (GST) from Schistosoma haematobium (Sh28GST) was expressed e...

  14. Note: Light output enhanced fast response and low afterglow 6Li glass scintillator as potential down-scattered neutron diagnostics for inertial confinement fusion

    International Nuclear Information System (INIS)

    The characteristics of an APLF80+3Ce scintillator are presented. Its sufficiently fast decay profile, low afterglow, and an improved light output compared to the recently developed APLF80+3Pr, were experimentally demonstrated. This scintillator material holds promise for applications in neutron imaging diagnostics at the energy regions of 0.27 MeV of DD fusion down-scattered neutron peak at the world's largest inertial confinement fusion facilities such as the National Ignition Facility and the Laser Megajoule.

  15. Short fusion

    CERN Multimedia

    2002-01-01

    French and UK researchers are perfecting a particle accelerator technique that could aid the quest for fusion energy or make X-rays that are safer and produce higher-resolution images. Led by Dr Victor Malka from the Ecole Nationale Superieure des Techniques Avancees in Paris, the team has developed a better way of accelerating electrons over short distances (1 page).

  16. Magnetic fusion

    International Nuclear Information System (INIS)

    This document is a detailed lecture on thermonuclear fusion. The basic physics principles are recalled and the technological choices that have led to tokamaks or stellarators are exposed. Different aspects concerning thermonuclear reactors such as safety, economy and feasibility are discussed. Tore-supra is described in details as well as the ITER project

  17. Color Facial Image Enhancement Based on Gray Redundancy and the Fusion of Wavelet Transform%基于灰度冗余和小波变换融合的彩色人脸图像增强

    Institute of Scientific and Technical Information of China (English)

    薛丹丹; 王鉴; 韩焱

    2012-01-01

    According to the condition that due to the different light ,the face image has too dark or bright to see and to recognize, this article presents a color facial image enhancement algorithm which is based on gray redundancy and the fusion of wavelet transform. First, in order to enhance the image contrastit remove the gray redundancy through the linear mapping of gray level; and then with the special fusion rule, it carries on the image fusion based on the wavelet transformation. Finally, it realizes the color image enhancement, overcoms the source image iu brightness flaws, avoid the information loss and improve the definition of the image-%针对不同光照下的人脸图像存在过暗或过亮,视觉效果不好、不易识别的情况,提出了一种基于灰度冗余和小波变换融合的彩色图像增强算法.该方法通过灰度级的线性映射去除灰度冗余,增强图像的对比度,再通过基于小波变换的图像融合方法,对低频和高频采用不同的融合准则,实现了彩色图像的增强,克服了原图像在亮度和细节上的缺陷,又避免了信息损失,提高了图像的清晰度.

  18. Angular momentum distributions in subbarrier fusion reactions

    International Nuclear Information System (INIS)

    Interest in subbarrier heavy-ion fusion was stimulated by the realization that subbarrier fusion cross sections were enhanced by many orders of magnitude over what would be expected from quantum mechanical one-dimensional barrier penetration. This review focuses on the angular momentum (spin) distributions in heavy-ion fusion reactions. Experimental probes, theoretical considerations, and a comparison of experimental results with model calculations are given. 86 refs., 10 figs

  19. Magnetic fusion; La fusion magnetique

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    This document is a detailed lecture on thermonuclear fusion. The basic physics principles are recalled and the technological choices that have led to tokamaks or stellarators are exposed. Different aspects concerning thermonuclear reactors such as safety, economy and feasibility are discussed. Tore-supra is described in details as well as the ITER project.

  20. The influence of transfer reactions on the sub-barrier fusion enhancement in the systems {sup 58.64}Ni +, {sup 92,100}Mo

    Energy Technology Data Exchange (ETDEWEB)

    Rehm, K.E.; Jiang, C.L.; Esbensen, H. [and others

    1995-08-01

    High resolution experiments performed during the past few years demonstrated that the various reaction modes occurring in heavy ion collisions can strongly influence each other. This interrelation of the different reaction modes brings a nuclear structure dependence to the fusion and deep-inelastic channels that were previously described in the framework of pure statistical models. In order to fully understand the interrelation between these reaction channels, a complete set of measurements including elastic and inelastic scattering, few-nucleon transfer and fusion is required. In continuation of our earlier measurements of the fusion cross sections in the system {sup 58,64}Ni + {sup 92,100}Mo we finished the studies of the quasielastic process in these systems. The experiments were done in inverse reaction kinematics using the split-pole spectrograph with its hybrid focal-plane detector for particle identification. The experiments with {sup 100}Mo beams were performed previously. First test runs with {sup 92}Mo showed the possible interference with {sup 98}Mo ions which could be eliminated by using the 13{sup +} charge state from the ECR source. The data from these experiments were completely analyzed. The smallest transfer cross sections are observed for the systems {sup 64}Ni + {sup 100}Mo and {sup 58}Ni + {sup 92}Mo, i.e., the most neutron-rich and neutron-deficient systems, respectively. For the other systems, {sup 64}Ni + {sup 92}Mo and {sup 58}Ni + {sup 100}Mo, the transfer cross sections at energies close to the barrier are about of equal magnitude. This observation does not correlate with the deviation of the experimental fusion cross sections from the coupled-channels predictions. While for {sup 58}Ni + {sup 100}Mo discrepancies between the experimental and theoretical fusion cross sections are observed, the system {sup 64}Ni + {sup 92}Mo which shows about the same transfer yields, is quite well described by the coupled-channels calculations.

  1. Distributed multi-sensor fusion

    Science.gov (United States)

    Scheffel, Peter; Fish, Robert; Knobler, Ron; Plummer, Thomas

    2008-03-01

    McQ has developed a broad based capability to fuse information in a geographic area from multiple sensors to build a better understanding of the situation. The paper will discuss the fusion architecture implemented by McQ to use many sensors and share their information. This multi sensor fusion architecture includes data sharing and analysis at the individual sensor, at communications nodes that connect many sensors together, at the system server/user interface, and across multi source information available through networked services. McQ will present a data fusion architecture that integrates a "Feature Information Base" (FIB) with McQ's well known Common Data Interchange Format (CDIF) data structure. The distributed multi sensor fusion provides enhanced situation awareness for the user.

  2. Fusion rules of equivariantizations of fusion categories

    OpenAIRE

    Burciu, Sebastian; Natale, Sonia

    2012-01-01

    We determine the fusion rules of the equivariantization of a fusion category $\\mathcal{C}$ under the action of a finite group $G$ in terms of the fusion rules of $\\mathcal{C}$ and group-theoretical data associated to the group action. As an application we obtain a formula for the fusion rules in an equivariantization of a pointed fusion category in terms of group-theoretical data. This entails a description of the fusion rules in any braided group-theoretical fusion category.

  3. Carpal Fusion

    OpenAIRE

    Jalal Jalalshokouhi; Mohammad Hossein Herischi; Shahyar Pashaei; Ali Akbar Ameri

    2012-01-01

    Carpal fusion may be seen in hereditary and nonhereditary conditions such as acrocallosal syndrome,acromegaly, Apert syndrome, arthrogryposis, Carpenter syndrome, chromosomal abnormalities, ectrodactyly-ectodermal dysplasia-cleft (EEC) syndrome, the F form of acropectorovertebral dysgenesis or the F syndrome, fetal alcohol syndrome, Holt-Oram syndrome, Leopard syndrome, multiple synostosis syndrome, oligosyndactyly syndrome, Pfeiffer-like syndrome, scleroderma, split hand and foot malformatio...

  4. Cold Fusion

    OpenAIRE

    Zhang, Chu; Yue, Manyu; Yu, Huanzhang; Chen, Cheng

    2006-01-01

    Science can often result in technologies which can solve energy problems in societies. On March 23, 1989, two scientists Stanley Pons and Martin Fleischmann claimed at a press conference that they had been able to perform nuclear fusion at room temperature. Their claim was quickly investigated and checked by many scientists around the world. Their discovery generated a heated debate in the scientific literature and magazines in the next few years, and their work was criticized for being unsci...

  5. Fusion fundamentals

    International Nuclear Information System (INIS)

    The United States and other countries are in the process of formulating energy policies to meet an anticipated world-wide shortage of fuel supplies. Immediate attention will necessarily focus on fuel conservation and on stretching-out conventional energy technologies (oil, gas, coal and light water reactors). The underlying concern of all policy, however, must be to guard against the emergence of a time gap between the exhaustion of conventional fuels and the availability of appropriate inexhaustible energy sources in the next century. The only known candidates for providing the bulk of the world's long-term energy needs are fusion, solar and fission breeder reactors. These are all still in the development stage and, consequently, it is too early to precisely identify their relative advantages and disadvantages. To assure that fusion will be available as one of the long-term option, the US is continuing a dedicated research and development program to take fusion from its present state to a point where its commercial viability can be ascertained. Similar programs are being carried out in the USSR, Europe, and Japan. 2 tables

  6. Laser fusion

    International Nuclear Information System (INIS)

    In this paper, the physics of laser fusion is described on an elementary level. The irradiated matter consists of a dense inner core surrounded by a less dense plasma corona. The laser radiation is mainly absorbed in the outer periphery of the plasma. The absorbed energy is transported inward to the ablation surface where plasma flow is created. Due to this plasma flow, a sequence of inward going shock waves and heat waves are created, resulting in the compression and heating of the core to high density and temperature. The interaction physics between laser and matter leading to thermonuclear burn is summarized by the following sequence of events: Laser absorption → Energy transport → Compression → Nuclear Fusion. This scenario is shown in particular for a Nd:laser with a wavelength of 1 μm. The wavelength scaling of the physical processes is also discussed. In addition to the laser-plasma physics, the Nd high power pulsed laser is described. We give a very brief description of the oscillator, the amplifiers, the spatial filters, the isolators and the diagnostics involved. Last, but not least, the concept of reactors for laser fusion and the necessary laser system are discussed. (author)

  7. Vibrational excitation of simple molecular ions in resonant and under-resonant strong laser fields: Dissociation and ionization of ppe and pde; laser-enhanced nuclear fusion in ddμ and dtμ

    International Nuclear Information System (INIS)

    The dynamics of vibrational excitation of ppe, pde, ddμ and dtμ in very intense laser fields (I0 ∼ 1014 W/cm2 for ppe and pde, and I0 ∼ 1020 and 1022 W/cm2 for ddμ and dtμ) is studied by the numerical solution of the time-dependent Schroedinger equations with the explicit treatment of the nuclear motion and the electron or muon motion beyond the Born-Oppenheimer approximation. The laser frequencies used were chosen (i) on resonance with the corresponding |v = 0> →|v = 1> transitions: ω = ω10, and (ii) two times smaller: ω = ω10/2. It has been found that the strong-field vibrational excitation with the latter, under-resonant frequencies (ω = ω10/2), is much more efficient than that with the resonant ones. In particular, on the timescale of 120 femtoseconds (fs) the dissociation yield of pde is about 25% at ω = ω10/2, but only about 3.8% at ω = ω10. It has been also shown that, similarly to an electron in ppe and pde, a muon in ddμ and dtμ also follows the fast oscillations of the resonant X-ray laser fields, despite its very large mass and extremely high laser frequencies, ω ∼ 10 a.u. The X-ray enhancement of the nuclear fusion in ddμ and dtμ is studied on the attosecond (as) timescale. It is found, in particular, that the energy released from the dt fusion in dtμ can by 8.02 GeV exceed the energy required to produce a usable μ- and the energy of the 300 as X-ray laser pulse used to enhance the fusion

  8. A phycocyanin·phellandrene synthase fusion enhances recombinant protein expression and β-phellandrene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria).

    Science.gov (United States)

    Formighieri, Cinzia; Melis, Anastasios

    2015-11-01

    Cyanobacteria can be exploited as photosynthetic platforms for heterologous generation of terpene hydrocarbons with industrial applications. Transformation of Synechocystis and heterologous expression of the β-phellandrene synthase (PHLS) gene alone is necessary and sufficient to confer to Synechocystis the ability to divert intermediate terpenoid metabolites and to generate the monoterpene β-phellandrene during photosynthesis. However, terpene synthases, including the PHLS, have a slow Kcat (low Vmax) necessitating high levels of enzyme concentration to enable meaningful rates and yield of product formation. Here, a novel approach was applied to increase the PHLS protein expression alleviating limitations in the rate and yield of β-phellandrene product generation. Different PHLS fusion constructs were generated with the Synechocystis endogenous cpcB sequence, encoding for the abundant in cyanobacteria phycocyanin β-subunit, expressed under the native cpc operon promoter. In one of these constructs, the CpcB·PHLS fusion protein accumulated to levels approaching 20% of the total cellular protein, i.e., substantially higher than expressing the PHLS protein alone under the same endogenous cpc promoter. The CpcB·PHLS fusion protein retained the activity of the PHLS enzyme and catalyzed β-phellandrene synthesis, yielding an average of 3.2 mg product g(-1) dry cell weight (dcw) versus the 0.03 mg g(-1)dcw measured with low-expressing constructs, i.e., a 100-fold yield improvement. In conclusion, the terpene synthase fusion-protein approach is promising, as, in this case, it substantially increased the amount of the PHLS in cyanobacteria, and commensurately improved rates and yield of β-phellandrene hydrocarbons production in these photosynthetic microorganisms. PMID:26410450

  9. Fusion materials irradiations at MaRIE'S fission fusion facility

    International Nuclear Information System (INIS)

    Los Alamos National Laboratory's proposed signature facility, MaRIE, will provide scientists and engineers with new capabilities for modeling, synthesizing, examining, and testing materials of the future that will enhance the USA's energy security and national security. In the area of fusion power, the development of new structural alloys with better tolerance to the harsh radiation environments expected in fusion reactors will lead to improved safety and lower operating costs. The Fission and Fusion Materials Facility (F3), one of three pillars of the proposed MaRIE facility, will offer researchers unprecedented access to a neutron radiation environment so that the effects of radiation damage on materials can be measured in situ, during irradiation. The calculated radiation damage conditions within the F3 match, in many respects, that of a fusion reactor first wall, making it well suited for testing fusion materials. Here we report in particular on two important characteristics of the radiation environment with relevancy to radiation damage: the primary knock-on atom spectrum and the impact of the pulse structure of the proton beam on temporal characteristics of the atomic displacement rate. With respect to both of these, analyses show that F3 has conditions that are consistent with those of a steady-state fusion reactor first wall.

  10. Fusion materials irradiations at MaRIE's fission fusion facility

    International Nuclear Information System (INIS)

    Los Alamos National Laboratory's proposed signature facility, MaRIE, will provide scientists and engineers with new capabilities for modeling, synthesizing, examining, and testing materials of the future that will enhance the USA's energy security and national security. In the area of fusion power, the development of new structural alloys with better tolerance to the harsh radiation environments expected in fusion reactors will lead to improved safety and lower operating costs. The Fission and Fusion Materials Facility (F3), one of three pillars of the proposed MaRIE facility, will offer researchers unprecedented access to a neutron radiation environment so that the effects of radiation damage on materials can be measured in-situ, during irradiation. The calculated radiation damage conditions within the F3 match, in many respects, that of a fusion reactor first wall, making it well suited for testing fusion materials. Here we report in particular on two important characteristics of the radiation environment with relevancy to radiation damage: the primary knock-on atom spectrum and the impact of the pulse structure of the proton beam on temporal characteristics of the atomic displacement rate. With respect to both of these, analyses show the F3 has conditions that are consistent with those of a steady-state fusion reactor first wall.

  11. Expression and its significance of caveolin-1 in liver and gallbladder of gallstone mice model%微囊蛋白-1在胆囊结石模型小鼠肝脏胆囊中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    刘姗; 陈洪潭; 陈李华; 徐根云; 许国强

    2015-01-01

    目的 探讨微囊蛋白-1在致石饲料诱导的小鼠胆囊胆固醇结石症形成中的作用及其可能的机制.方法 以结石易感C57BL/6小鼠为研究对象,分别给予对照组共6只和实验组共6只小鼠普通饲料和致石饲料饲养4周,检测小鼠胆囊胆固醇结石形成状况、血脂、胆汁脂质含量变化.采用实时定量PCR法和Western蛋白印迹法检测小鼠肝脏及胆囊组织中微囊蛋白-1、清道夫受体B族Ⅰ型(SR-BI)的mRNA和蛋白表达水平和变化.采用t检验比较两组均数.结果 致石饲料饲养后4周,实验组小鼠胆囊成石率为100%,血脂水平明显升高;胆汁中胆固醇成分明显增加,胆盐减少.与对照组相比,微囊蛋白1在实验组小鼠肝脏和胆囊组织中mRNA和蛋白水平表达均显著下调(肝脏mRNA相对定量值为0.53±0.13比1.00±0.32,t=3.330;蛋白相对定量值为0.39±0.07比0.92±0.06,t=10.280;胆囊mRNA相对定量值为0.40±0.22比1.00±0.22,t=3.823;蛋白相对定量值为1.04±0.07比1.34±0.04,t=6.367,P均<0.01).实验组小鼠肝胆组织的SR-BⅠ蛋白和mRNA相对定量与对照组相比差异均无统计学意义(P均>0.05).结论 肝胆组织中微囊蛋白-1的mRNA和蛋白表达水平变化可能影响实验小鼠肝胆组织中的脂质代谢和胆固醇转运,在胆囊胆固醇结石的形成中发挥着作用.%Objective To investigate the role and possible mechanism of caveolin-1 (CAV1) in the forming of cholesterol gallstone in mice fed with lithogenic diet.Methods Cholesterol gallstone susceptible C57BL/6 mice were study objects.The mice of control group (n=6) and experiment group (n=6) were fed with normal diet and lithogenic diet for four weeks respectively.The condition of cholesterol gallstone forming,changes of serum lipid and bile composition were measured,and the expressions of CAV1 and scavenger receptor classB member Ⅰ (SR-BⅠ) at mRNA and protein level in the liver and gallbladder were detected by realtime

  12. Atomic physics in Inertial Confinement Fusion (ICF)

    International Nuclear Information System (INIS)

    The US Inertial Confinement Fusion Program plans to start advanced pulsed power experiments at the end of 2001. The enhancement of plasma diagnostics technique and target design demand a profound understanding of atomic processes. Advances are reviewed

  13. COLOR CONTRAST ENHANCENT METHOD TO IMPRROVE TARGET DETECTABILITY IN NIGHT VISION FUSION%提高夜视融合目标可探测性的颜色对比度增强方法

    Institute of Scientific and Technical Information of China (English)

    殷松峰; 曹良才; 杨华; 谭峭峰; 何庆声; 凌永顺; 金国藩

    2009-01-01

    Requirements on color image fusion for situation awareness and target detection tasks were analyzed based on the characteristics of human visual system.Then a night vision fusion method was presented to improve target detectability by enhancing color contrast between the target and the background.The proposed fusion method relates hot and cold targets to the colors of intense red and cyan, respectively.According to the characteristics of infrared images, a color contrast enhancement ratio related to the divergence of the intensity of each pixel from the mean intensity of the infrared image was utilized to enhance color contrast between the target and the background.The enhancing method offsets the disadvantage of color transfer method in color contrast and improves target detectability.Experimental results demonstrate that the method can not only pop out both hot and cold targets, but also keep details of the background well.The situation awareness and target detectability can be improved by the method.%根据人眼视觉系统特性,分析了场景理解和目标探测对彩色融合的要求,并据此提出了一种通过颜色对比度增强来提高目标可探测性的夜视融合方法.该方法使热目标呈红色,冷目标呈蓝绿色;根据红外图像特征,引入了一种和红外图像各像素亮度与图像平均亮度的偏离相关的颜色对比度增强因子,利用该因子可增强目标与背景的颜色对比度,弥补颜色传递彩色融合方法在颜色对比度上的不足,能有效提高目标可探测性.实验表明该方法既能突出红外目标,又能保持丰富的背景细节,在增强场景理解的同时提高了目标可探测性.

  14. Nuclear Fusion Fuel Cycle Research Perspectives

    International Nuclear Information System (INIS)

    As a part of the International Thermonuclear Experimental Reactor (ITER) Project, we at the Korea Atomic Energy Research Institute (KAERI) and our National Fusion Research Institute (NFRI) colleagues are investigating nuclear fusion fuel cycle hardware including a nuclear fusion fuel Storage and Delivery System (SDS). To have a better knowledge of the nuclear fusion fuel cycle, we present our research efforts not only on SDS but also on the Fuel Supply System (FS), Tokamak Exhaust Processing System (TEP), Isotope Separation System (ISS), and Detritiation System (DS). To have better knowledge of the nuclear fusion fuel cycle, we presented our research efforts not only on SDS but also on the Fuel Supply System (FS), Tokamak Exhaust Processing System (TEP), Isotope Separation System (ISS), and Detritiation System (DS). Our efforts to enhance the tritium confinement will be continued for the development of cleaner nuclear fusion power plants

  15. Nuclear Fusion Fuel Cycle Research Perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Hongsuk; Koo, Daeseo; Park, Jongcheol; Kim, Yeanjin [KAERI, Daejeon (Korea, Republic of); Yun, Sei-Hun [National Fusion Research Institute, Daejeon (Korea, Republic of)

    2015-05-15

    As a part of the International Thermonuclear Experimental Reactor (ITER) Project, we at the Korea Atomic Energy Research Institute (KAERI) and our National Fusion Research Institute (NFRI) colleagues are investigating nuclear fusion fuel cycle hardware including a nuclear fusion fuel Storage and Delivery System (SDS). To have a better knowledge of the nuclear fusion fuel cycle, we present our research efforts not only on SDS but also on the Fuel Supply System (FS), Tokamak Exhaust Processing System (TEP), Isotope Separation System (ISS), and Detritiation System (DS). To have better knowledge of the nuclear fusion fuel cycle, we presented our research efforts not only on SDS but also on the Fuel Supply System (FS), Tokamak Exhaust Processing System (TEP), Isotope Separation System (ISS), and Detritiation System (DS). Our efforts to enhance the tritium confinement will be continued for the development of cleaner nuclear fusion power plants.

  16. Prospects for improved fusion reactors

    International Nuclear Information System (INIS)

    Ideally, a new energy source must be capable of displacing old energy sources while providing both economic opportunities and enhanced environmental benefits. The attraction of an essentially unlimited fuel supply has generated a strong impetus to develop advanced fission breeders and, even more strongly, the exploitation of nuclear fusion. Both fission and fusion systems trade a reduced fuel charge for a more capital-intensive plant needed to utilize a cheaper and more abundant fuel. Results from early conceptual designs of fusion power plants, however, indicated a capital intensiveness that could override cost savings promised by an inexpensive fuel cycle. Early warnings of these problems appeared, and generalized routes to more economically attractive systems have been suggested; specific examples have also recently been given. Although a direct reduction in the cost (and mass) of the fusion power core (FPC, i.e., plasma chamber, first wall, blanket, shield, coils, and primary structure) most directly reduces the overall cost of fusion power, with the mass power density (MPD, ratio of net electric power to FPC mass, kWe/tonne) being suggested as a figure-of-merit in this respect, other technical, safety/environmental, and institutional issues also enter into the definition of and direction for improved fusion concepts. These latter issues and related tradeoffs are discussed

  17. Carpal Fusion

    Directory of Open Access Journals (Sweden)

    Jalal Jalalshokouhi*

    2012-05-01

    Full Text Available Carpal fusion may be seen in hereditary and nonhereditary conditions such as acrocallosal syndrome,acromegaly, Apert syndrome, arthrogryposis, Carpenter syndrome, chromosomal abnormalities, ectrodactyly-ectodermal dysplasia-cleft (EEC syndrome, the F form of acropectorovertebral dysgenesis or the F syndrome, fetal alcohol syndrome, Holt-Oram syndrome, Leopard syndrome, multiple synostosis syndrome, oligosyndactyly syndrome, Pfeiffer-like syndrome, scleroderma, split hand and foot malformation, Stickler syndrome, thalidomide embryopathy, Turner syndrome and many other conditions as mentioned in Rubinstein-Taybi's book. Sometimes there is no known causative disease.Diagnosis is usually made by plain X-ray during studying a syndrome or congenital disease or could be an incidental finding like our patients. Hand bone anomalies are more common in syndromes or other congenital or non-hereditary conditions, but polydactyly, syndactyly or oligodactyly and carpal fusions are interesting. X-ray is the modality of choice, but MRI and X-ray CT with multiplanar reconstructions may be used for diagnosis.

  18. Reaction dynamics for fusion of weakly-bound nuclei

    OpenAIRE

    Hagino, K.; Vitturi, A.

    2004-01-01

    We discuss several open problems of fusion reactions induced by weakly bound nuclei. For this purpose, we solve a one dimensional three-body Hamiltonian with the coupled-channels formalism. We show that the continuum-continuum couplings substantially reduce the total fusion probability at energies above the barrier compared with the no-breakup case, although the fusion probability remains enhanced at subbarrier energies. We then discuss a role of transfer process in fusion of weakly bound nuc...

  19. Characterisation of Novel Cytochrome P450-fusion enzymes

    OpenAIRE

    Luciakova, Dominika

    2015-01-01

    Characterisation of Novel Cytochrome P450-fusion enzymesSeptember 2014This study focuses on the characterisation of three novel cytochrome P450-partner (P450-fusion) enzymes of unknown structure and function. Despite several well-established P450 functions, new structures of P450s are published frequently, with the P450-redox partner fusion systems being among the most discussed, due to their enhanced activity and biotechnological potential. Other, more intriguing, P450-fusions involve partne...

  20. Enhanced expression of full-length human cytomegalovirus fusion protein in non-swelling baculovirus-infected cells with a minimal fed-batch strategy.

    Directory of Open Access Journals (Sweden)

    Marco Patrone

    Full Text Available Human cytomegalovirus congenital infection represents an unmet medical issue and attempts are ongoing to develop an effective vaccine. The virion fusion players of this enveloped virus are the natural targets to achieve this goal and to develop novel anti-viral therapies. The secreted ectodomain of the viral fusion factor glycoprotein B (gB has been exploited so far as an alternative to the cumbersome expression of the wild type trans-membrane protein. In the soluble form, gB showed encouraging but limited potential as antigen candidate calling for further efforts. Here, the exhaustive evaluation of the Baculovirus/insect cell expression system has been coupled to an orthogonal screening for expression additives to produce full-length gB. In detail, rapamycin was found to prolong gB intracellular accumulation while inhibiting the infection-induced cell swelling. Not obvious to predict, this inhibition did not affect Baculovirus growth, revealing that the virus-induced cell size increase is a dispensable side phenotype. In parallel, a feeding strategy for the limiting nutrient cysteine has been set up which improved gB stability. This multi-modal scheme allowed the production of full-length, mutation-free gB in the milligram scale. The recombinant full-length gB obtained was embedded into a stable mono-dispersed particle substantially larger than the protein trimer itself, according to the reported association of this protein with detergent-resistant lipid domains.

  1. Revitalizing Fusion via Fission Fusion

    Science.gov (United States)

    Manheimer, Wallace

    2001-10-01

    Existing tokamaks could generate significant nuclear fuel. TFTR, operating steady state with DT might generate enough fuel for a 300 MW nuclear reactor. The immediate goals of the magnetic fusion program would necessarily shift from a study of advanced plasma regimes in larger sized devices, to mostly known plasmas regimes, but at steady state or high duty cycle operation in DT plasmas. The science and engineering of breeding blankets would be equally important. Follow on projects could possibly produce nuclear fuel in large quantity at low price. Although today there is strong opposition to nuclear power in the United States, in a 21st century world of 10 billion people, all of whom will demand a middle class life style, nuclear energy will be important. Concern over greenhouse gases will also drive the world toward nuclear power. There are studies indicating that the world will need 10 TW of carbon free energy by 2050. It is difficult to see how this can be achieved without the breeding of nuclear fuel. By using the thorium cycle, proliferation risks are minimized. [1], [2]. 1 W. Manheimer, Fusion Technology, 36, 1, 1999, 2.W. Manheimer, Physics and Society, v 29, #3, p5, July, 2000

  2. Recent developments concerning the fusion; Developpements recents sur la fusion

    Energy Technology Data Exchange (ETDEWEB)

    Jacquinot, J. [CEA/Cadarache, Dept. de Recherches sur la Fusion Controlee, DRFC, 13 - Saint Paul lez Durance (France); Andre, M. [CEA/DAM Ile de France, 91 - Bruyeres Le Chatel (France); Aymar, R. [ITER Joint Central Team Garching, Muenchen (Germany)] [and others

    2000-09-04

    Organized the 9 march 2000 by the SFEN, this meeting on the european program concerning the fusion, showed the utility of the exploitation and the enhancement of the actual technology (JET, Tore Supra, ASDEX) and the importance of the Europe engagement in the ITER program. The physical stakes for the magnetic fusion have been developed with a presentation of the progresses in the knowledge of the stability limits. A paper on the inertial fusion was based on the LMJ (Laser MegaJoule) project. The two blanket concepts chosen in the scope of the european program on the tritium blankets, have been discussed. These concepts will be validated by irradiation tests in the ITER-FEAT and adapted for a future reactor. (A.L.B.)

  3. Catalysed fusion

    CERN Document Server

    Farley, Francis

    2012-01-01

    A sizzling romance and a romp with subatomic particles at CERN. Love, discovery and adventure in the city where nations meet and beams collide. Life in a large laboratory. As always, the challenges are the same. Who leads? Who follows? Who succeeds? Who gets the credit? Who gets the women or the men? Young Jeremy arrives in CERN and joins the quest for green energy. Coping with baffling jargon and manifold dangers, he is distracted by radioactive rats, lovely ladies and an unscrupulous rival. Full of doubts and hesitations, he falls for a dazzling Danish girl, who leads him astray. His brilliant idea leads to a discovery and a new route to cold fusion. But his personal life is scrambled. Does it bring fame or failure? Tragedy or triumph?

  4. Recent developments concerning the fusion

    International Nuclear Information System (INIS)

    Organized the 9 march 2000 by the SFEN, this meeting on the european program concerning the fusion, showed the utility of the exploitation and the enhancement of the actual technology (JET, Tore Supra, ASDEX) and the importance of the Europe engagement in the ITER program. The physical stakes for the magnetic fusion have been developed with a presentation of the progresses in the knowledge of the stability limits. A paper on the inertial fusion was based on the LMJ (Laser MegaJoule) project. The two blanket concepts chosen in the scope of the european program on the tritium blankets, have been discussed. These concepts will be validated by irradiation tests in the ITER-FEAT and adapted for a future reactor. (A.L.B.)

  5. Fracto-fusion

    International Nuclear Information System (INIS)

    As a cold fusion mechanism we investigated a fracto-fusion by which reacting particles are accelerated by the electric field generated between the crack surfaces in a crystal and the beam fusion occurs. By assuming the possible magnitude of the potential difference we calculated the fusion rate and energy multiplication factor. These results are consistent with cold fusion experiments. On the basis of a simple model it is conjectured that necessary electric potential difference to accelerate particles can be generated even in a metal crystal with rather low resistivity, and we conclude that the fracto-fusion mechanism can explain the cold fusion phenomena successfully. (author)

  6. Materials research for fusion

    Science.gov (United States)

    Knaster, J.; Moeslang, A.; Muroga, T.

    2016-05-01

    Fusion materials research started in the early 1970s following the observation of the degradation of irradiated materials used in the first commercial fission reactors. The technological challenges of fusion energy are intimately linked with the availability of suitable materials capable of reliably withstanding the extremely severe operational conditions of fusion reactors. Although fission and fusion materials exhibit common features, fusion materials research is broader. The harder mono-energetic spectrum associated with the deuterium-tritium fusion neutrons (14.1 MeV compared to fusion-relevant neutron source for materials testing is an essential pending step in fusion roadmaps. Structural materials development, together with research on functional materials capable of sustaining unprecedented power densities during plasma operation in a fusion reactor, have been the subject of decades of worldwide research efforts underpinning the present maturity of the fusion materials research programme.

  7. Fusion energy

    International Nuclear Information System (INIS)

    The main purpose of the International Thermonuclear Experimental Reactor (ITER) is to develop an experimental fusion reactor through the united efforts of many technologically advanced countries. The ITER terms of reference, issued jointly by the European Community, Japan, the USSR, and the United States, call for an integrated international design activity and constitute the basis of current activities. Joint work on ITER is carried out under the auspices of the International Atomic Energy Agency (IAEA), according to the terms of quadripartite agreement reached between the European Community, Japan, the USSR, and the United States. The site for joint technical work sessions is at the MaxPlanck Institute of Plasma Physics. Garching, Federal Republic of Germany. The ITER activities have two phases: a definition phase performed in 1988 and the present design phase (1989--1990). During the definition phase, a set of ITER technical characteristics and supporting research and development (R ampersand D) activities were developed and reported. The present conceptual design phase of ITER lasts until the end of 1990. The objectives of this phase are to develop the design of ITER, perform a safety and environmental analysis, develop site requirements, define future R ampersand D needs, and estimate cost, manpower, and schedule for construction and operation. A final report will be submitted at the end of 1990. This paper summarizes progress in the ITER program during the 1989 design phase

  8. Fusion Energy from the electric utilities perspective: Fusion Innovation Industry Forum

    International Nuclear Information System (INIS)

    The paper presents the different future energy scenarios envisaged and the so called Power Generation Fleet Transition in which Fusion Energy could play an important role. A review of the R and D and Innovation main drivers in the electric sector is outline, with a detail description of the main issues and strategic challenges in the medium and short term. The worldwide historical involvement of electric utilities in Fusion is presented and revised under the new USA Utilities technical assessment carried out by the Electric Power Research Institute EPRI. The paper also presents the work done in the last few years by the European Fusion Industry Innovation Forum FIIF-MB in order to to evaluate a wide range of fusion concepts from the utility standpoint, to enhance utilities perspective on fusion, to provide guidance to Government Bodies and national Energy strategies for fusion-utilities and finally to establish a basis for communication and cooperation in fusion for utilities standpoint. Finally the paper comments the utilities challenges pointed out by the Fusion electricity: a road map to the realization of fusion energy report issued this year by the European Fusion Development Agreement EFDA.

  9. Sonluminescence and cold fusion

    Institute of Scientific and Technical Information of China (English)

    TVPrevenslik

    1996-01-01

    A theory of sonoluminescence(SL) proposed asserts bubbles collapse in a pancake shape almost isothermally at constant volume.SL induced hot fusion at -107℃is most likely impossible.By the proposed theory,the bubbles are treated as miniature IRasers with the dimension between pancake faces equal to the half wavelength of the standing wave in resonance with the IRaser cavity dimensions.Before collapse,the IRasers are filled with 5-10eV of IR radiation from the Plank energy in the ambient surroundings.During collapse,the IUR radiation is trapped within the IRaser by the high IR reflectivity of the bubble wall water molecules.As the IRaser cavity dimension dercreases,the cavity resonant frequency increases.The bubble wall molecules undergo a continuous population inversion by always being pumped to higher energy states that favor stimulated emission instead of absorption.IRaser resonant intensification produces 5-10MeV,i.e.,-106UV photons with 5-10eV.On average,SL induced cold fuison does not occur although a few X-ray photons are produced.If the IR thermal energy before collapse is enhanced to a total energy of 10keV.say by exciting the vibration modes of the water molecules at 2.73um with a HF laser,collapse produces an average X-ray energy of about 10keV.unlike hot fusion ,Sl induced cold fusion is not impossible.

  10. Enhanced research in ground-penetrating radar and multisensor fusion with application to the detection and visualization of buried waste. Final report

    International Nuclear Information System (INIS)

    Recognizing the difficulty and importance of the landfill remediation problems faced by DOE, and the fact that no one sensor alone can provide complete environmental site characterization, a multidisciplinary team approach was chosen for this project. The authors have developed a multisensor fusion approach that is suitable for the wide variety of sensors available to DOE, that allows separate detection algorithms to be developed and custom-tailored to each sensor. This approach is currently being applied to the Geonics EM-61 and Coleman step-frequency radar data. High-resolution array processing techniques were developed for detecting and localizing buried waste containers. A soil characterization laboratory facility was developed using a HP-8510 network analyzer and near-field coaxial probe. Both internal and external calibration procedures were developed for de-embedding the frequency-dependent soil electrical parameters from the measurements. Dispersive soil propagation modeling algorithms were also developed for simulating wave propagation in dispersive soil media. A study was performed on the application of infrared sensors to the landfill remediation problem, particularly for providing information on volatile organic compounds (VOC's) in the atmosphere. A dust-emission lidar system is proposed for landfill remediation monitoring. Design specifications are outlined for a system which could be used to monitor dust emissions in a landfill remediation effort. The detailed results of the investigations are contained herein

  11. Galactose supplementation enhance sialylation of recombinant Fc-fusion protein in CHO cell: an insight into the role of galactosylation in sialylation.

    Science.gov (United States)

    Liu, Jintao; Wang, Jie; Fan, Li; Chen, Xinning; Hu, Dongdong; Deng, Xiancun; Poon, H Fai; Wang, Haibin; Liu, Xuping; Tan, Wen-Song

    2015-07-01

    Sialic acid levels of therapeutic glycoprotein play an important role in plasma half-life. An undesirable decrease of sialic acid content was observed when we increased Fc-fusion protein productivity fourfold in a GS-CHO cell line by bioprocess optimization. We investigated the potential mechanism for the sialic acid content reduction. We found that limited nucleotide sugar precursor and the extracellular sialidase were not responsible for the reduction of the sialic acid content after titer improvement. Oligosaccharide analysis revealed that the lack of protein galactosylation was the potential cause for the reduction of sialic acid content. Thus we validated this notion by evaluated galactose supplementation in 2 L bioreactors. Cell culture performance was not impacted by addition of up to 40 mM galactose except for the glucose consumption rate. Addition of 20 mM galactose to the bioreactor resulted in the increase of 44 % for total sialic acid content and 20.3 % for sialylated glycans. These data were further validated when the process was run on 200 L scaled bioreactor. These data together show that the galactosylation plays an apparent role in sialylation in our current system. PMID:25931375

  12. Enhanced research in ground-penetrating radar and multisensor fusion with application to the detection and visualization of buried waste. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Devney, A.J.; DiMarzio, C.; Kokar, M.; Miller, E.L.; Rappaport, C.M.; Weedon, W.H.

    1996-05-14

    Recognizing the difficulty and importance of the landfill remediation problems faced by DOE, and the fact that no one sensor alone can provide complete environmental site characterization, a multidisciplinary team approach was chosen for this project. The authors have developed a multisensor fusion approach that is suitable for the wide variety of sensors available to DOE, that allows separate detection algorithms to be developed and custom-tailored to each sensor. This approach is currently being applied to the Geonics EM-61 and Coleman step-frequency radar data. High-resolution array processing techniques were developed for detecting and localizing buried waste containers. A soil characterization laboratory facility was developed using a HP-8510 network analyzer and near-field coaxial probe. Both internal and external calibration procedures were developed for de-embedding the frequency-dependent soil electrical parameters from the measurements. Dispersive soil propagation modeling algorithms were also developed for simulating wave propagation in dispersive soil media. A study was performed on the application of infrared sensors to the landfill remediation problem, particularly for providing information on volatile organic compounds (VOC`s) in the atmosphere. A dust-emission lidar system is proposed for landfill remediation monitoring. Design specifications are outlined for a system which could be used to monitor dust emissions in a landfill remediation effort. The detailed results of the investigations are contained herein.

  13. Effects of entrance channel on fusion probability in hot fusion reactions

    CERN Document Server

    Zhu, Long; Huang, Ching-Yuan; Zhang, Feng-Shou

    2016-01-01

    Within the framework of the dinuclear system (DNS) model, the fusion reactions leading to the compound nuclei 274Hs and 286Cn are investigated. The fusion probability as a function of DNS excitation energy is studied. The calculated results are in good agreement with the available experimental data. The obtained results show that the fusion probabilities are obviously enhanced for the reactions located at high place in potential energy surface, although these reactions may have small values of mass asymmetry. It is found that the enhancement is due to the large potential energy of the initial DNS.

  14. Radiolytic production of chemical fuels in fusion reactor systems

    International Nuclear Information System (INIS)

    Miley's energy flow diagram for fusion reactor systems is extended to include radiolytic production of chemical fuel. Systematic study of the economics and the overall efficiencies of fusion reactor systems leads to a criterion for evaluating the potential of radiolytic production of chemical fuel as a means of enhancing the performance of a fusion reactor system. The ecumenicity of the schema is demonstrated by application to (1) tokamaks, (2) mirror machines, (3) theta-pinch reactors, (4) laser-heated solenoids, and (5) inertially confined, laser-pellet devices. Pure fusion reactors as well as fusion-fission hybrids are considered

  15. Bemerkungen zur "kalten Fusion"

    CERN Document Server

    Kuehne, R W

    2006-01-01

    Steven Jones et al. reported to have observed nuclear fusion at room temperature. They observed this "cold fusion" by electrolyzing heavy water. Later experiments confirmed these observations. These experiments confirmed the generation of strong electric fields within the deuterided metals. These electric fields accelerate the deuterons to keV energies and allow the observed nuclear fusion. Roman Sioda and I suggested a theoretical description of this nuclear fusion. Our "extended micro hot fusion" scenario explains how nuclear fusion can be generated over a long time within deuterided metals. Moreover we predicted the explosion of large pieces of deuterided metals. This article reviews the "cold fusion" work of Steven Jones et al. and discusses the fracto-fusion scenario. I show that the extended micro hot fusion scenario can explain the observed neutron emissions, neutron bursts, and heat bursts.

  16. Image fusion in dual energy computed tomography for detection of various anatomic structures - Effect on contrast enhancement, contrast-to-noise ratio, signal-to-noise ratio and image quality

    Energy Technology Data Exchange (ETDEWEB)

    Paul, Jijo, E-mail: jijopaul1980@gmail.com [Department of Diagnostic Radiology, Goethe University Hospital, Theodor-Stern-Kai 7, 60590 Frankfurt am Main (Germany); Department of Biophysics, Goethe University, Max von Laue-Str.1, 60438 Frankfurt am Main (Germany); Bauer, Ralf W. [Department of Diagnostic Radiology, Goethe University Hospital, Theodor-Stern-Kai 7, 60590 Frankfurt am Main (Germany); Maentele, Werner [Department of Biophysics, Goethe University, Max von Laue-Str.1, 60438 Frankfurt am Main (Germany); Vogl, Thomas J. [Department of Diagnostic Radiology, Goethe University Hospital, Theodor-Stern-Kai 7, 60590 Frankfurt am Main (Germany)

    2011-11-15

    Objective: The purpose of this study was to evaluate image fusion in dual energy computed tomography for detecting various anatomic structures based on the effect on contrast enhancement, contrast-to-noise ratio, signal-to-noise ratio and image quality. Material and methods: Forty patients underwent a CT neck with dual energy mode (DECT under a Somatom Definition flash Dual Source CT scanner (Siemens, Forchheim, Germany)). Tube voltage: 80-kV and Sn140-kV; tube current: 110 and 290 mA s; collimation-2 x 32 x 0.6 mm. Raw data were reconstructed using a soft convolution kernel (D30f). Fused images were calculated using a spectrum of weighting factors (0.0, 0.3, 0.6 0.8 and 1.0) generating different ratios between the 80- and Sn140-kV images (e.g. factor 0.6 corresponds to 60% of their information from the 80-kV image, and 40% from the Sn140-kV image). CT values and SNRs measured in the ascending aorta, thyroid gland, fat, muscle, CSF, spinal cord, bone marrow and brain. In addition, CNR values calculated for aorta, thyroid, muscle and brain. Subjective image quality evaluated using a 5-point grading scale. Results compared using paired t-tests and nonparametric-paired Wilcoxon-Wilcox-test. Results: Statistically significant increases in mean CT values noted in anatomic structures when increasing weighting factors used (all P {<=} 0.001). For example, mean CT values derived from the contrast enhanced aorta were 149.2 {+-} 12.8 Hounsfield Units (HU), 204.8 {+-} 14.4 HU, 267.5 {+-} 18.6 HU, 311.9 {+-} 22.3 HU, 347.3 {+-} 24.7 HU, when the weighting factors 0.0, 0.3, 0.6, 0.8 and 1.0 were used. The highest SNR and CNR values were found in materials when the weighting factor 0.6 used. The difference CNR between the weighting factors 0.6 and 0.3 was statistically significant in the contrast enhanced aorta and thyroid gland (P = 0.012 and P = 0.016, respectively). Visual image assessment for image quality showed the highest score for the data reconstructed using the

  17. Image fusion in dual energy computed tomography for detection of various anatomic structures - Effect on contrast enhancement, contrast-to-noise ratio, signal-to-noise ratio and image quality

    International Nuclear Information System (INIS)

    Objective: The purpose of this study was to evaluate image fusion in dual energy computed tomography for detecting various anatomic structures based on the effect on contrast enhancement, contrast-to-noise ratio, signal-to-noise ratio and image quality. Material and methods: Forty patients underwent a CT neck with dual energy mode (DECT under a Somatom Definition flash Dual Source CT scanner (Siemens, Forchheim, Germany)). Tube voltage: 80-kV and Sn140-kV; tube current: 110 and 290 mA s; collimation-2 x 32 x 0.6 mm. Raw data were reconstructed using a soft convolution kernel (D30f). Fused images were calculated using a spectrum of weighting factors (0.0, 0.3, 0.6 0.8 and 1.0) generating different ratios between the 80- and Sn140-kV images (e.g. factor 0.6 corresponds to 60% of their information from the 80-kV image, and 40% from the Sn140-kV image). CT values and SNRs measured in the ascending aorta, thyroid gland, fat, muscle, CSF, spinal cord, bone marrow and brain. In addition, CNR values calculated for aorta, thyroid, muscle and brain. Subjective image quality evaluated using a 5-point grading scale. Results compared using paired t-tests and nonparametric-paired Wilcoxon-Wilcox-test. Results: Statistically significant increases in mean CT values noted in anatomic structures when increasing weighting factors used (all P ≤ 0.001). For example, mean CT values derived from the contrast enhanced aorta were 149.2 ± 12.8 Hounsfield Units (HU), 204.8 ± 14.4 HU, 267.5 ± 18.6 HU, 311.9 ± 22.3 HU, 347.3 ± 24.7 HU, when the weighting factors 0.0, 0.3, 0.6, 0.8 and 1.0 were used. The highest SNR and CNR values were found in materials when the weighting factor 0.6 used. The difference CNR between the weighting factors 0.6 and 0.3 was statistically significant in the contrast enhanced aorta and thyroid gland (P = 0.012 and P = 0.016, respectively). Visual image assessment for image quality showed the highest score for the data reconstructed using the weighting factor 0

  18. Pseudorabies Virus Glycoprotein M Inhibits Membrane Fusion

    OpenAIRE

    Klupp, Barbara G.; Nixdorf, Ralf; Mettenleiter, Thomas C.

    2000-01-01

    A transient transfection-fusion assay was established to investigate membrane fusion mediated by pseudorabies virus (PrV) glycoproteins. Plasmids expressing PrV glycoproteins under control of the immediate-early 1 promoter-enhancer of human cytomegalovirus were transfected into rabbit kidney cells, and the extent of cell fusion was quantitated 27 to 42 h after transfection. Cotransfection of plasmids encoding PrV glycoproteins B (gB), gD, gH, and gL resulted in formation of polykaryocytes, as...

  19. Data fusion using geographic managed objects

    DEFF Research Database (Denmark)

    Kjems, Erik

    2014-01-01

    a frustrating one. So what have we gained from CAD. Certainly a lot, but mostly the possibility to combine and fuse projects. Simulating future use and behaviour, revealing design issues and failures before actually built. Still data fusion is a relatively new challenge albeit quite obvious trying to assemble...... models coming from different systems and vendors representing different professional domains. This paper discusses data exchange and data fusion in general and presents a new development, which gives the possibility to enhance data as intelligent objects opening a whole new paradigm for both data...... exchange and data fusion....

  20. Value of retrospective image fusion of {sup 18}F-FDG PET and MRI for preoperative staging of head and neck cancer: Comparison with PET/CT and contrast-enhanced neck MRI

    Energy Technology Data Exchange (ETDEWEB)

    Kanda, Tomonori [Department of Radiology, Kobe University Graduate School of Medicine, Kobe (Japan); Department of Radiology, Hyogo Cancer Center, Hyogo (Japan); Kitajima, Kazuhiro, E-mail: kitajima@med.kobe-u.ac.jp [Department of Radiology, Kobe University Graduate School of Medicine, Kobe (Japan); Suenaga, Yuko; Konishi, Jyunya [Department of Radiology, Kobe University Graduate School of Medicine, Kobe (Japan); Sasaki, Ryohei [Division of Radiation Oncology, Department of Radiology, Kobe University Graduate School of Medicine, Kobe (Japan); Morimoto, Koichi; Saito, Miki; Otsuki, Naoki; Nibu, Ken-ichi [Department of Otolaryngology-Head and Neck Surgery, Kobe University Graduate School of Medicine, Kobe (Japan); Sugimura, Kazuro [Department of Radiology, Kobe University Graduate School of Medicine, Kobe (Japan)

    2013-11-01

    Purpose: To assess the clinical value of retrospective image fusion of neck MRI and {sup 18}F-fluorodeoxyglucose ({sup 18}F-FDG) PET for locoregional extension and nodal staging of neck cancer. Materials and methods: Thirty patients with carcinoma of the oral cavity or hypopharynx underwent PET/CT and contrast-enhanced neck MRI for initial staging before surgery including primary tumor resection and neck dissection. Diagnostic performance of PET/CT, MRI, and retrospective image fusion of PET and MRI (fused PET/MRI) for assessment of the extent of the primary tumor (T stage) and metastasis to regional lymph nodes (N stage) was evaluated. Results: Accuracy for T status was 87% for fused PET/MRI and 90% for MRI, thus proving significantly superior to PET/CT, which had an accuracy of 67% (p = 0.041 and p = 0.023, respectively). Accuracy for N status was 77% for both fused PET/MRI and PET/CT, being superior to MRI, which had an accuracy of 63%, although the difference was not significant (p = 0.13). On a per-level basis, the sensitivity, specificity and accuracy for detection of nodal metastasis were 77%, 96% and 93% for both fused PET/MRI and PET/CT, compared with 49%, 99% and 91% for MRI, respectively. The differences for sensitivity (p = 0.0026) and accuracy (p = 0.041) were significant. Conclusion: Fused PET/MRI combining the individual advantages of MRI and PET is a valuable technique for assessment of staging neck cancer.

  1. Review of fusion synfuels

    International Nuclear Information System (INIS)

    Thermonuclear fusion offers an inexhaustible source of energy for the production of hydrogen from water. Depending on design, electric generation efficiencies of approx. 40 to 60% and hydrogen production efficiencies by high-temperature electrolysis of approx. 50 to 65% are projected for fusion reactors using high-temperatures blankets. Fusion/coal symbiotic systems appear economically promising for the first generation of commercial fusion synfuels plants. Coal production requirements and the environmental effects of large-scale coal usage would be greatly reduced by a fusion/coal system. In the long term, there could be a gradual transition to an inexhaustible energy system based solely on fusion

  2. Monocrotaline pyrrole-induced megalocytosis of lung and breast epithelial cells: Disruption of plasma membrane and Golgi dynamics and an enhanced unfolded protein response

    International Nuclear Information System (INIS)

    The pyrrolizidine alkaloid monocrotaline (MCT) initiates pulmonary hypertension by inducing a 'megalocytosis' phenotype in target pulmonary arterial endothelial, smooth muscle and Type II alveolar epithelial cells. In cultured endothelial cells, a single exposure to the pyrrolic derivative of monocrotaline (MCTP) results in large cells with enlarged endoplasmic reticulum (ER) and Golgi and increased vacuoles. However, these cells fail to enter mitosis. Largely based upon data from endothelial cells, we proposed earlier that a disruption of the trafficking and mitosis-sensor functions of the Golgi (the 'Golgi blockade' hypothesis) may represent the subcellular mechanism leading to MCTP-induced megalocytosis. In the present study, we investigated the applicability of the Golgi blockade hypothesis to epithelial cells. MCTP induced marked megalocytosis in cultures of lung A549 and breast MCF-7 cells. This was associated with a change in the distribution of the cis-Golgi scaffolding protein GM130 from a discrete juxtanuclear localization to a circumnuclear distribution consistent with an anterograde block of GM130 trafficking to/through the Golgi. There was also a loss of plasma membrane caveolin-1 and E-cadherin, cortical actin together with a circumnuclear accumulation of clathrin heavy chain (CHC) and α-tubulin. Flotation analyses revealed losses/alterations in the association of caveolin-1, E-cadherin and CHC with raft microdomains. Moreover, megalocytosis was accompanied by an enhanced unfolded protein response (UPR) as evidenced by nuclear translocation of Ire1α and glucose regulated protein 58 (GRP58/ER-60/ERp57) and a circumnuclear accumulation of PERK kinase and protein disulfide isomerase (PDI). These data further support the hypothesis that an MCTP-induced Golgi blockade and enhanced UPR may represent the subcellular mechanism leading to enlargement of ER and Golgi and subsequent megalocytosis

  3. Nuclear plus coulomb interference scattering between energetic fusion products and fuel particles

    International Nuclear Information System (INIS)

    Some effects such as hadronic elastic scattering,nuclear plus Coulomb interference scattering and propagation of fusion products become pronounced in D-3He fusion plasma with high temperature while Coulomb interactions of energetic ions produced by fusion reaction and plasma electrons get weaker. A fraction of background fuel ions due to the knock of fusion products becomes superheat tail ions in the Maxwellian distribution, therefore D-3He fusion reaction is enhanced

  4. Fusion Canada issue 23

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program highlighting in this issue TdeV tokamak updates, fusion research in Korea, CCFM program review, TdeV divertor plasma, and CFFTP program review. 4 figs

  5. Fusion Canada issue 27

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program highlighting in this issue ITER reactor siting, a major upgrade for TdeV tokamak, Ceramic Breeders: new tritium mapping technique and Joint Fusion Symposium. 2 figs

  6. Fusion Canada issue 20

    International Nuclear Information System (INIS)

    Fusion Canada's publication of the National Fusion Program. Included in this issue is the CFFTP Industrial Impact Study, CCFM/TdeV Update:helium pumping, research funds, and deuterium in beryllium - high temperature behaviour. 3 figs

  7. Fusion Canada issue 6

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program. Included in this issue is a funding report for CFFTP, a technical update for Tokamak de Varennes and a network for university research by the National Fusion Program. 4 figs

  8. Fusion hindrance and roles of shell effects in superheavy mass region

    CERN Document Server

    Aritomo, Y

    2006-01-01

    We present the first attempt of systematically investigating the effects of shell correction energy for a dynamical process, which includes fusion, fusion-fission and quasi-fission processes. In the superheavy mass region, for the fusion process, shell correction energy plays a very important role and enhances the fusion probability when the colliding partner has a strong shell structure. By analyzing the trajectory in three-dimensional coordinate space with the Langevin equation, we reveal the mechanism of the enhancement of the fusion probability caused by `cold fusion valleys'. The temperature dependence of shell correction energy is considered.

  9. Bemerkungen zur "kalten Fusion"

    OpenAIRE

    Kuehne, Rainer W.

    2006-01-01

    Steven Jones et al. reported to have observed nuclear fusion at room temperature. They observed this "cold fusion" by electrolyzing heavy water. Later experiments confirmed these observations. These experiments confirmed the generation of strong electric fields within the deuterided metals. These electric fields accelerate the deuterons to keV energies and allow the observed nuclear fusion. Roman Sioda and I suggested a theoretical description of this nuclear fusion. Our "extended micro hot f...

  10. Fusion to the Lysosome Targeting Signal of the Invariant Chain Alters the Processing and Enhances the Immunogenicity of HIV-1 Reverse Transcriptase

    Science.gov (United States)

    Starodubova, E. S.; Isaguliants, M. G.; Kuzmenko, Y. V.; Latanova, A. A.; Krotova, O. A.; Karpov, V. L.

    2014-01-01

    Intracellular processing of the antigen encoded by a DNA vaccine is one of the key steps in generating an immune response. Immunization with DNA constructs targeted to the endosomal-lysosomal compartments and to the MHC class II pathway can elicit a strong immune response. Herein, the weakly immunogenic reverse transcriptase of HIV-1 was fused to the minimal lysosomal targeting motif of the human MHC class II invariant chain. The motif fused to the N-terminus shifted the enzyme intracellular localization and accelerated its degradation. Degradation of the chimeric protein occurred predominantly in the lysosomal compartment. BALB/c mice immunized with the plasmid encoding the chimeric protein demonstrated an enhanced immune response, in the form of an increased antigen-specific production of Th1 cytokines, INF-γ and IL-2, by mouse splenocytes. Moreover, the majority of the splenocytes secreted both cytokines; i.e., were polyfunctional. These findings suggest that retargeting of the antigen to the lysosomes enhances the immune response to DNA vaccine candidates with low intrinsic immunogenicity. PMID:24772328

  11. Fusion technology program

    International Nuclear Information System (INIS)

    The report summarizes work performed in the following areas: system and safety studies for fusion reactors; nuclear data for fusion reactors; neutronics calculations for fusion reactors; radiation damage of vanadium alloys and stainless steel 316; facility for in-pile crack growth measurement; niobium tin magnet for Sultan - stage II; development of NET conductor; and development of ceramic tritium breeding materials

  12. Cold fusion research

    International Nuclear Information System (INIS)

    I am pleased to forward to you the Final Report of the Cold Fusion Panel. This report reviews the current status of cold fusion and includes major chapters on Calorimetry and Excess Heat, Fusion Products and Materials Characterization. In addition, the report makes a number of conclusions and recommendations, as requested by the Secretary of Energy

  13. Towards cognitive image fusion

    NARCIS (Netherlands)

    Toet, A.; Hogervorst, M.A.; Nikolov, S.G.; Lewis, J.J.; Dixon, T.D.; Bull, D.R.; Canagarajah, C.N.

    2010-01-01

    The increasing availability and deployment of imaging sensors operating in multiple spectral bands has led to a large research effort in image fusion, resulting in a plethora of pixel-level image fusion algorithms. However, the cognitive aspects of multisensor image fusion have not received much att

  14. Towards cognitive image fusion

    NARCIS (Netherlands)

    Toet, A.; Hogervorst, M.A.; Nikolov, S.G.; Lewis, J.; Dixon, T.; Bull, D.; Canagarajah, N.

    2007-01-01

    The increasing availability and deployment of imaging sensors operating in multiple spectral bands has led to a large research effort in image fusion, resulting in a plethora of pixel-level image fusion algorithms. However, the cognitive aspects of multisensor image fusion have not received much att

  15. Fusion Canada issue 18

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program. Included in this issue is a report on the ITER agreement signed with the EDA, the robotic maintenance for NET, the CFFTP Fusion Pilot Study, the new IEA joint programs on environment, safety and economic aspects of fusion power, and a review by the CCFM advisory committee. 3 figs

  16. Fusion Canada issue 17

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program. Included in this issue is a report on increased funding for the Canadian Fusion Program, news of the compact Toroid fuelling gun, an update on Tokamak de Varennes, the Canada - U.S. fusion meeting, measurements of plasma flow velocity, and replaceable Tokamak divertors. 4 figs

  17. Magneto-Inertial Fusion

    International Nuclear Information System (INIS)

    In this community white paper, we describe an approach to achieving fusion which employs a hybrid of elements from the traditional magnetic and inertial fusion concepts, called magneto-inertial fusion (MIF). The status of MIF research in North America at multiple institutions is summarized including recent progress, research opportunities, and future plans

  18. Conditions Favorable for the Somatic Embryogenesis in Carrot Cell Culture Enhance Expression of the roIC Promoter-GUS Fusion Gene 1

    Science.gov (United States)

    Fujii, Nobuharu; Uchimiya, Hirofumi

    1991-01-01

    We obtained carrot (Daucus carota) cells possessing the 5′-noncoding sequence of the ORF12 gene (roIC) of TL-DNA of the Ri plasmid and a structural gene of bacterial β-glucuronidase by Agrobacterium-mediated transformation. When such cells were cultured in medium containing 2,4-dichlorophenoxyacetic acid, substantial reduction in β-glucuronidase activity was observed. Upon transferring the cells from a 2,4-D-containing medium to one devoid of 2,4-dichlorophenoxyacetic acid, enhanced expression of β-glucuronidase in somatic embryo development was recorded. Activation by gibberillic acid and suppression by abscisic acid of β-glucuronidase activities, in concord with embryogenesis, were also noted. Images Figure 2 PMID:16667958

  19. Conditions Favorable for the Somatic Embryogenesis in Carrot Cell Culture Enhance Expression of the roIC Promoter-GUS Fusion Gene.

    Science.gov (United States)

    Fujii, N; Uchimiya, H

    1991-01-01

    We obtained carrot (Daucus carota) cells possessing the 5'-noncoding sequence of the ORF12 gene (roIC) of TL-DNA of the Ri plasmid and a structural gene of bacterial beta-glucuronidase by Agrobacterium-mediated transformation. When such cells were cultured in medium containing 2,4-dichlorophenoxyacetic acid, substantial reduction in beta-glucuronidase activity was observed. Upon transferring the cells from a 2,4-D-containing medium to one devoid of 2,4-dichlorophenoxyacetic acid, enhanced expression of beta-glucuronidase in somatic embryo development was recorded. Activation by gibberillic acid and suppression by abscisic acid of beta-glucuronidase activities, in concord with embryogenesis, were also noted. PMID:16667958

  20. Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G.

    Science.gov (United States)

    Anastasov, Nataša; Höfig, Ines; Mall, Sabine; Krackhardt, Angela M; Thirion, Christian

    2016-01-01

    Lentiviral vectors (LV) are widely used to successfully transduce cells for research and clinical applications. This optimized LV infection protocol includes a nontoxic poloxamer-based adjuvant combined with antibody-retargeted lentiviral particles. The novel poloxamer P338 demonstrates superior characteristics for enhancing lentiviral transduction over the best-in-class polybrene-assisted transduction. Poloxamer P338 exhibited dual benefits of low toxicity and high efficiency of lentiviral gene delivery into a range of different primary cell cultures. One of the major advantages of P338 is its availability in pharma grade and applicability as cell culture medium additive in clinical protocols. Lentiviral vectors pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G) can be produced to high titers and mediate high transduction efficiencies in vitro. For clinical applications the need for optimized transduction protocols, especially for transduction of primary T and stem cells, is high. The successful use of retronectin, the second lentivirus enhancer available as GMP material, requires the application of specific coating protocols not applicable in all processes, and results in the need of a relatively high multiplicity of infection (MOI) to achieve effective transduction efficiencies for hematopoietic cells (e.g., CD34+ hematopoietic stem cells). Cell specificity of lentiviral vectors was successfully increased by displaying different ratios of scFv-fused VSV-G glycoproteins on the viral envelope. The system has been validated with human CD30+ lymphoma cells, resulting in preferential gene delivery to CD30+ cells, which was increased fourfold in mixed cell cultures, by presenting scFv antibody fragments binding to respective surface markers. A combination of spinoculation and poloxamer-based chemical adjuvant increases the transduction of primary T-cells by greater than twofold. The combination of poloxamer-based and scFv-retargeted LVs increased

  1. Thermal Resonance Fusion

    OpenAIRE

    Dong, Bao-Guo

    2015-01-01

    We first show a possible mechanism to create a new type of nuclear fusion, thermal resonance fusion, i.e. low energy nuclear fusion with thermal resonance of light nuclei or atoms, such as deuterium or tritium. The fusion of two light nuclei has to overcome the Coulomb barrier between these two nuclei to reach up to the interacting region of nuclear force. We found nuclear fusion could be realized with thermal vibrations of crystal lattice atoms coupling with light atoms at low energy by reso...

  2. Fusion applications study: FAME

    Energy Technology Data Exchange (ETDEWEB)

    Schultz, K.R.

    1986-01-01

    Fusion has a wide spectrum of applications that appear technically possible and may become economically feasible. Near-term (approx. 2000) application for production of nuclear fuels and useful radioisotopes is an economically attractive possibility as soon as fusion is ready. Electricity production will remain a prime, large-scale application of fusion. In the longer term, as fossil fuels dwindle, production of hydrogen could become a major application. Additional applications some of which have not even been conceived of yet, will add to this potential richness and diversity of fusion. It is the purpose of the fusion applications study - FMAE - to innovate, investigate, and evaluate these potential applications.

  3. Magnetized target fusion and fusion propulsion.

    Energy Technology Data Exchange (ETDEWEB)

    Kirkpatrick, R. C. (Ronald C.)

    2001-01-01

    Magnetized target fusion (MTF) is a thermonuclear fusion concept that is intermediate between the two mainline approaches, magnetic confinement and inertial confinement fusion (MCF and ICF). MTF incorporates some aspects of each and offers advantages over each of the mainline approaches. First, it provides a means of reducing the driver power requirements, thereby admitting a wider range of drivers than ICF. Second, the magnetic field is only used for insulation, not confinement, and the plasma is wall confined, so that plasma instabilities are traded in for hydrodynamic instabilities. However, the degree of compression required to reach fusion conditions is lower than for ICF, so that hydrodynamic instabilities are much less threatening. The standoff driver innovation proposes to dynamically form the target plasma and a gaseous shell that compresses and confines the target plasma. Therefore, fusion target fabrication is traded in for a multiplicity of plasma guns, which must work in synchrony. The standoff driver embodiment of MTF leads to a fusion propulsion system concept that is potentially compact and lightweight. We will discuss the underlying physics of MTF and some of the details of the fusion propulsion concept using the standoff driver approach. We discuss here the optimization of an MTF target design for space propulsion.

  4. Fusion using radioactive ion beams

    Indian Academy of Sciences (India)

    A M Vinodkumar

    2010-07-01

    The capture-fission cross-section is measured for the collision of the massive nucleus 132Sn with 96Zr at near-barrier energies and compared with the collision of 124Sn with 96Zr. This study gives insight into fusion enhancement and hindrance in systems involving neutron-rich nuclei. The dinuclear system model (DNS) calculations describe the excitation function reasonably well and if we use the barrier heights predicted by this model we can conclude that fusion hindrance (represented by extra push energy) is greater for the more neutron-rich systems. The fusion excitation function for 9Li+70Zn and 9Li+208Pb systems are measured for near-barrier energies using ISAC1 and ISAC2 Facilities at TRIUMF. The -emitting evaporation residues (211−214At) are stopped in the 208Pb target and their decay is measured. The measured excitation function shows evidence for large enhancements in the sub-barrier energies, which is not accounted by current theoretical models. Suppression of the above-barrier cross-section with respect to these theoretical models are also seen.

  5. Influence of in-complete fusion on complete fusion: observation of large fusion suppression at ≅ 5-7 MeV/nucleon

    International Nuclear Information System (INIS)

    Various studies showed that the complete fusion is quantitatively enhanced up to the critical angular momentum. However, even at small increase in input-angular momentum and beam energy may influence the contribution of complete fusion giving rise to an increase in incomplete fusion fraction. Such reaction products attribute additional production cross-sections than predicted by theoretical model code PACE2 based on Monte-Carlo simulation. In the present contribution, energy and mass asymmetry dependent complete fusion suppression factor has been reported for 16O + 159Tb systems at ≅ 5-7 MeV/nucleon

  6. Viral membrane fusion

    Energy Technology Data Exchange (ETDEWEB)

    Harrison, Stephen C., E-mail: harrison@crystal.harvard.edu

    2015-05-15

    Membrane fusion is an essential step when enveloped viruses enter cells. Lipid bilayer fusion requires catalysis to overcome a high kinetic barrier; viral fusion proteins are the agents that fulfill this catalytic function. Despite a variety of molecular architectures, these proteins facilitate fusion by essentially the same generic mechanism. Stimulated by a signal associated with arrival at the cell to be infected (e.g., receptor or co-receptor binding, proton binding in an endosome), they undergo a series of conformational changes. A hydrophobic segment (a “fusion loop” or “fusion peptide”) engages the target-cell membrane and collapse of the bridging intermediate thus formed draws the two membranes (virus and cell) together. We know of three structural classes for viral fusion proteins. Structures for both pre- and postfusion conformations of illustrate the beginning and end points of a process that can be probed by single-virion measurements of fusion kinetics. - Highlights: • Viral fusion proteins overcome the high energy barrier to lipid bilayer merger. • Different molecular structures but the same catalytic mechanism. • Review describes properties of three known fusion-protein structural classes. • Single-virion fusion experiments elucidate mechanism.

  7. Viral membrane fusion

    International Nuclear Information System (INIS)

    Membrane fusion is an essential step when enveloped viruses enter cells. Lipid bilayer fusion requires catalysis to overcome a high kinetic barrier; viral fusion proteins are the agents that fulfill this catalytic function. Despite a variety of molecular architectures, these proteins facilitate fusion by essentially the same generic mechanism. Stimulated by a signal associated with arrival at the cell to be infected (e.g., receptor or co-receptor binding, proton binding in an endosome), they undergo a series of conformational changes. A hydrophobic segment (a “fusion loop” or “fusion peptide”) engages the target-cell membrane and collapse of the bridging intermediate thus formed draws the two membranes (virus and cell) together. We know of three structural classes for viral fusion proteins. Structures for both pre- and postfusion conformations of illustrate the beginning and end points of a process that can be probed by single-virion measurements of fusion kinetics. - Highlights: • Viral fusion proteins overcome the high energy barrier to lipid bilayer merger. • Different molecular structures but the same catalytic mechanism. • Review describes properties of three known fusion-protein structural classes. • Single-virion fusion experiments elucidate mechanism

  8. Fusion technology 1992

    International Nuclear Information System (INIS)

    The aim of the biennial series of symposia on the title subject, organized by the European Fusion Laboratories, is the exchange of information on the design, construction and operation of fusion experiments and on the technology being developed for the next step devices and fusion reactors. The coverage of the volume includes the technological aspects of fusion reactors in relation to new developments, this forming a guideline for the definition of future work. These proceedings comprise three volumes and contain both the invited lectures and contributed papers presented at the symposium which was attended by 569 participants from around the globe. The 343 papers, including 12 invited papers, characterize the increasing interest of industry in the fusion programme, giving a broad and current overview on the progress and trends fusion technology is experiencing now, as well as indicating the future for fusion devices

  9. The fusion breeder

    International Nuclear Information System (INIS)

    The fusion breeder is a fusion reactor designed with special blankets to maximize the transmutation by 14 MeV neutrons of uranium-238 to plutonium or thorium to uranium-233 for use as a fuel for fission reactors. Breeding fissile fuels has not been a goal of the U.S. fusion energy program. This paper suggests it is time for a policy change to make the fusion breeder a goal of the U.S. fusion program and the U.S. nuclear energy program. There is wide agreement that many approaches will work and will produce fuel for five equal-sized LWRs, and some approach as many as 20 LWRs at electricity costs within 20% of those at today's price of uranium ($30/lb of U3O8). The blankets designed to suppress fissioning, called symbiotes, fusion fuel factories, or just fusion breeders, will have safety characteristics more like pure fusion reactors and will support as many as 15 equal power LWRs. The blankets designed to maximize fast fission of fertile material will have safety characteristics more like fission reactors and will support 5 LWRs. This author strongly recommends development of the fission suppressed blanket type, a point of view not agreed upon by everyone. There is, however, wide agreement that, to meet the market price for uranium which would result in LWR electricity within 20% of today's cost with either blanket type, fusion components can cost severalfold more than would be allowed for pure fusion to meet the goal of making electricity alone at 20% over today's fission costs. Also widely agreed is that the critical-pathitem for the fusion breeder is fusion development itself; however, development of fusion breeder specific items (blankets, fuel cycle) should be started now in order to have the fusion breeder by the time the rise in uranium prices forces other more costly choices

  10. Parameters critical to muon-catalyzed fusion

    International Nuclear Information System (INIS)

    We have demonstrated that muon catalysis cycling rates increase rapidly with increasing deuterium-tritium gas temperatures and densities. Furthermore, muon-capture losses are significantly smaller than predicted before the experiments. There remains a significant gap between observation and theoretical expectation for the muon-alpha sticking probability in dense d-t mixtures. We have been able to achieve muon-catalyzed yields of 150 fusion/muon (average). While the fusion energy thereby released significantly exceeds expectations, enhancements by nearly a factor of twenty would be needed to realize energy applications for a pure (non-hybrid) muon-catalyzed fusion reactor. The process could be useful in tritium-breeding schemes. We have also explored a new form of cold nuclear fusion which occurs when hydrogen isotopes are loaded into metals. 22 refs., 10 figs

  11. Modelling of carbon transport in fusion devices: evidence of enhanced re-erosion of in-situ re-deposited carbon

    International Nuclear Information System (INIS)

    The paper presents new Monte-Carlo transport simulations of methane 13CH4 injected through a hole in a testlimiter and exposed to the edge plasma of TEXTOR. The results show that the spatial distribution of 13C re-deposited locally on the testlimiter surface can be modelled if the parameter S for the sticking of returning hydrocarbons 13CHy is set to zero or almost zero. This is interpreted as a negligible effective sticking of the returning hydrocarbon radicals due to the instantaneous re-erosion caused by the hydrogen carried with the CHy radicals ('self re-erosion'). However, the calculated local deposition efficiency of 13C, remains too high compared with the observed value. Therefore, in addition an enhanced yield for chemical erosion caused by the background hydrogen for the fresh re-deposits has to be assumed. Similar assumptions can reproduce also the high amount of carbon deposition found on the inner louvers in the MkIIa divertor configuration of JET and on the plasma-shadowed areas of the MkIIGB divertor

  12. Sub-barrier Fusion and Breakup of Light Halo Nuclei

    Science.gov (United States)

    Martel, I.; Wolski, R.; Standyło, L.; Acosta, L.; Aguado, J. L.; Angulo, C.; Berjillos, R.; Bolivar, J. P.; Dueñas, J. A.; Golovkov, M. S.; Keutgen, T.; Mazzocco, M.; Sánchez-Benítez, A. M.; Signorini, C.; Romoli, M.; Rusek, K.

    2011-10-01

    In this work we present new data for the sub-barrier fusion of the system 6He+206Pb obtained in the Centre de Recherches du Cyclotron (UCL), in Louvain-la-Neuve, Belgium. The preliminary results suggest the absence of fusion enhancement at sub-barrier energies.

  13. Materials research for fusion

    Science.gov (United States)

    Knaster, J.; Moeslang, A.; Muroga, T.

    2016-05-01

    Fusion materials research started in the early 1970s following the observation of the degradation of irradiated materials used in the first commercial fission reactors. The technological challenges of fusion energy are intimately linked with the availability of suitable materials capable of reliably withstanding the extremely severe operational conditions of fusion reactors. Although fission and fusion materials exhibit common features, fusion materials research is broader. The harder mono-energetic spectrum associated with the deuterium-tritium fusion neutrons (14.1 MeV compared to average for fission neutrons) releases significant amounts of hydrogen and helium as transmutation products that might lead to a (at present undetermined) degradation of structural materials after a few years of operation. Overcoming the historical lack of a fusion-relevant neutron source for materials testing is an essential pending step in fusion roadmaps. Structural materials development, together with research on functional materials capable of sustaining unprecedented power densities during plasma operation in a fusion reactor, have been the subject of decades of worldwide research efforts underpinning the present maturity of the fusion materials research programme.

  14. Economics of fusion research

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    1977-10-15

    This report provides the results of a study of methods of economic analysis applied to the evaluation of fusion research. The study recognizes that a hierarchy of economic analyses of research programs exists: standard benefit-cost analysis, expected value of R and D information, and expected utility analysis. It is shown that standard benefit-cost analysis, as commonly applied to research programs, is inadequate for the evaluation of a high technology research effort such as fusion research. A methodology for performing an expected value analysis is developed and demonstrated and an overview of an approach to perform an expected utility analysis of fusion research is presented. In addition, a potential benefit of fusion research, not previously identified, is discussed and rough estimates of its magnitude are presented. This benefit deals with the effect of a fusion research program on optimal fossil fuel consumption patterns. The results of this study indicate that it is both appropriate and possible to perform an expected value analysis of fusion research in order to assess the economics of a fusion research program. The results indicate further that the major area of benefits of fusion research is likely due to the impact of a fusion research program on optimal fossil fuel consumption patterns and it is recommended that this benefit be included in future assessments of fusion research economics.

  15. Economics of fusion research

    International Nuclear Information System (INIS)

    This report provides the results of a study of methods of economic analysis applied to the evaluation of fusion research. The study recognizes that a hierarchy of economic analyses of research programs exists: standard benefit-cost analysis, expected value of R and D information, and expected utility analysis. It is shown that standard benefit-cost analysis, as commonly applied to research programs, is inadequate for the evaluation of a high technology research effort such as fusion research. A methodology for performing an expected value analysis is developed and demonstrated and an overview of an approach to perform an expected utility analysis of fusion research is presented. In addition, a potential benefit of fusion research, not previously identified, is discussed and rough estimates of its magnitude are presented. This benefit deals with the effect of a fusion research program on optimal fossil fuel consumption patterns. The results of this study indicate that it is both appropriate and possible to perform an expected value analysis of fusion research in order to assess the economics of a fusion research program. The results indicate further that the major area of benefits of fusion research is likely due to the impact of a fusion research program on optimal fossil fuel consumption patterns and it is recommended that this benefit be included in future assessments of fusion research economics

  16. Benchmarking of data fusion algorithms in support of earth observation based Antarctic wildlife monitoring

    Science.gov (United States)

    Witharana, Chandi; LaRue, Michelle A.; Lynch, Heather J.

    2016-03-01

    Remote sensing is a rapidly developing tool for mapping the abundance and distribution of Antarctic wildlife. While both panchromatic and multispectral imagery have been used in this context, image fusion techniques have received little attention. We tasked seven widely-used fusion algorithms: Ehlers fusion, hyperspherical color space fusion, high-pass fusion, principal component analysis (PCA) fusion, University of New Brunswick fusion, and wavelet-PCA fusion to resolution enhance a series of single-date QuickBird-2 and Worldview-2 image scenes comprising penguin guano, seals, and vegetation. Fused images were assessed for spectral and spatial fidelity using a variety of quantitative quality indicators and visual inspection methods. Our visual evaluation elected the high-pass fusion algorithm and the University of New Brunswick fusion algorithm as best for manual wildlife detection while the quantitative assessment suggested the Gram-Schmidt fusion algorithm and the University of New Brunswick fusion algorithm as best for automated classification. The hyperspherical color space fusion algorithm exhibited mediocre results in terms of spectral and spatial fidelities. The PCA fusion algorithm showed spatial superiority at the expense of spectral inconsistencies. The Ehlers fusion algorithm and the wavelet-PCA algorithm showed the weakest performances. As remote sensing becomes a more routine method of surveying Antarctic wildlife, these benchmarks will provide guidance for image fusion and pave the way for more standardized products for specific types of wildlife surveys.

  17. Recycling fusion materials

    International Nuclear Information System (INIS)

    The inherent safety and environmental advantages of fusion power in comparison with other energy sources play an important role in the public acceptance. No waste burden for future generations is therefore one of the main arguments to decide for fusion power. The waste issue has thus been studied in several documents and the final conclusion of which it is stated that there is no permanent disposal waste needed if recycling is applied. But recycling of fusion reactor materials is far to be obvious regarding mostly the very high specific activity of the materials to be handled, the types of materials and the presence of tritium. The main objective of research performed by SCK-CEN is to study the possible ways of recycling fusion materials and analyse the challenges of the materials management from fusion reactors, based on current practices used in fission reactors and the requirements for the manufacture of fusion equipment

  18. Muon Catalyzed Fusion

    Science.gov (United States)

    Armour, Edward A.G.

    2007-01-01

    Muon catalyzed fusion is a process in which a negatively charged muon combines with two nuclei of isotopes of hydrogen, e.g, a proton and a deuteron or a deuteron and a triton, to form a muonic molecular ion in which the binding is so tight that nuclear fusion occurs. The muon is normally released after fusion has taken place and so can catalyze further fusions. As the muon has a mean lifetime of 2.2 microseconds, this is the maximum period over which a muon can participate in this process. This article gives an outline of the history of muon catalyzed fusion from 1947, when it was first realised that such a process might occur, to the present day. It includes a description of the contribution that Drachrnan has made to the theory of muon catalyzed fusion and the influence this has had on the author's research.

  19. Fusion of Nonionic Vesicles

    DEFF Research Database (Denmark)

    Bulut, Sanja; Oskolkova, M. Z.; Schweins, R.;

    2010-01-01

    We present an experimental study of vesicle fusion using light and neutron scattering to monitor fusion events. Vesicles are reproducibly formed with an extrusion procedure using an single amphiphile triethylene glycol mono-n-decyl ether in water. They show long-term stability for temperatures...... around 20 C, but at temperatures above 26 C we observe an increase in the scattered intensity due to fusion. The system is unusually well suited for the study of basic mechanisms of vesicle fusion. The vesicles are flexible with a bending rigidity of only a few k(H)T. The monolayer spontaneous curvature......, Ho, depends strongly on temperature in a known way and is thus tunable. For temperatures where H-0 > 0 vesicles tyre long-term stable, while in the range H-0 fusion rate increases the more negative the Spontaneous curvature Through a quantitative;analysis of the fusion rate we arrive tit...

  20. Magnetized Target Fusion (MTF): Principles, Status, and International Collaboration

    Energy Technology Data Exchange (ETDEWEB)

    Kirkpatrick, R.C.

    1998-11-16

    Magnetized target fusion (MTF) is an approach to thermonuclear fusion that is intermediate between the two extremes of inertial and magnetic confinement. Target plasma preparation is followed by compression to fusion conditions. The use of a magnetic field to reduce electron thermal conduction and potentially enhance DT alpha energy deposition allows the compression rate to be drastically reduced relative to that for inertial confinement fusion. This leads to compact systems with target driver power and intensity requirements that are orders of magnitude lower than for ICF. A liner on plasma experiment has been proposed to provide a firm proof of principle for MTF.

  1. Multivariate Chemical Image Fusion of Vibrational Spectroscopic Imaging Modalities.

    Science.gov (United States)

    Gowen, Aoife A; Dorrepaal, Ronan M

    2016-01-01

    Chemical image fusion refers to the combination of chemical images from different modalities for improved characterisation of a sample. Challenges associated with existing approaches include: difficulties with imaging the same sample area or having identical pixels across microscopic modalities, lack of prior knowledge of sample composition and lack of knowledge regarding correlation between modalities for a given sample. In addition, the multivariate structure of chemical images is often overlooked when fusion is carried out. We address these challenges by proposing a framework for multivariate chemical image fusion of vibrational spectroscopic imaging modalities, demonstrating the approach for image registration, fusion and resolution enhancement of chemical images obtained with IR and Raman microscopy. PMID:27384549

  2. Status of fusion maintenance

    International Nuclear Information System (INIS)

    Effective maintenance will be an essential ingredient in determining fusion system productivity. This level of productivity will result only after close attention is paid to the entire system as an entity and appropriate integration of the elements is made. The status of fusion maintenance is reviewed in the context of the entire system. While there are many challenging developmental tasks ahead in fusion maintenance, the required technologies are available in several high-technology industries, including nuclear fission

  3. Filter Bank Fusion frames

    OpenAIRE

    Chebira, Amina; Fickus, Matthew; Mixon, Dustin G.

    2011-01-01

    In this paper we characterize and construct novel oversampled filter banks implementing fusion frames. A fusion frame is a sequence of orthogonal projection operators whose sum can be inverted in a numerically stable way. When properly designed, fusion frames can provide redundant encodings of signals which are optimally robust against certain types of noise and erasures. However, up to this point, few implementable constructions of such frames were known; we show how to construct them using ...

  4. Fusion reactor research

    International Nuclear Information System (INIS)

    This work covers four separate areas: (1) development of technology for processing liquid lithium from blankets, (2) investigation of hydrogen isotope permeation in candidate structural metals and alloys for near-term fusion reactors, (3) analytical studies encompassing fusion reactor thermal hydraulics, tritium facility design, and fusion reactor safety, and (4) studies involving dosimetry and damage analysis. Recent accomplishments in each of these areas are summarized

  5. Fusion11 Conference Summary

    International Nuclear Information System (INIS)

    This document is a summary account of the conference Fusion11, held in Saint Malo, France, May 2-6, 2011. There were 77 talks by experts in the field. The author comments on a few of the physics topics discussed during the presentations. His comments concern: new facilities, fusion cross-sections (particularly the barrier penetration model, optical potentials, the channel coupling, the barrier distribution, fusion hindrance and transfer channels), rare isotopes, clusters and superheavy elements

  6. Magnetic fusion technology

    CERN Document Server

    Dolan, Thomas J

    2014-01-01

    Magnetic Fusion Technology describes the technologies that are required for successful development of nuclear fusion power plants using strong magnetic fields. These technologies include: ? magnet systems, ? plasma heating systems, ? control systems, ? energy conversion systems, ? advanced materials development, ? vacuum systems, ? cryogenic systems, ? plasma diagnostics, ? safety systems, and ? power plant design studies. Magnetic Fusion Technology will be useful to students and to specialists working in energy research.

  7. Fusion research principles

    CERN Document Server

    Dolan, Thomas James

    2013-01-01

    Fusion Research, Volume I: Principles provides a general description of the methods and problems of fusion research. The book contains three main parts: Principles, Experiments, and Technology. The Principles part describes the conditions necessary for a fusion reaction, as well as the fundamentals of plasma confinement, heating, and diagnostics. The Experiments part details about forty plasma confinement schemes and experiments. The last part explores various engineering problems associated with reactor design, vacuum and magnet systems, materials, plasma purity, fueling, blankets, neutronics

  8. Fusion facility siting considerations

    International Nuclear Information System (INIS)

    Inherent in the fusion program's transition from hydrogen devices to commercial power machines is a general increase in the size and scope of succeeding projects. This growth will lead to increased emphasis on safety, environmental impact, and the external effects of fusion in general, and of each new device in particular. A critically important consideration in this regard is site selection. The purpose of this paper is to examine major siting issues that may affect the economics, safety, and environmental impact of fusion

  9. Magnetic fusion reactor economics

    International Nuclear Information System (INIS)

    An almost primordial trend in the conversion and use of energy is an increased complexity and cost of conversion systems designed to utilize cheaper and more-abundant fuels; this trend is exemplified by the progression fossil fission → fusion. The present projections of the latter indicate that capital costs of the fusion ''burner'' far exceed any commensurate savings associated with the cheapest and most-abundant of fuels. These projections suggest competitive fusion power only if internal costs associate with the use of fossil or fission fuels emerge to make them either uneconomic, unacceptable, or both with respect to expensive fusion systems. This ''implementation-by-default'' plan for fusion is re-examined by identifying in general terms fusion power-plant embodiments that might compete favorably under conditions where internal costs (both economic and environmental) of fossil and/or fission are not as great as is needed to justify the contemporary vision for fusion power. Competitive fusion power in this context will require a significant broadening of an overly focused program to explore the physics and simbiotic technologies leading to more compact, simplified, and efficient plasma-confinement configurations that reside at the heart of an attractive fusion power plant

  10. Frontiers in fusion research

    CERN Document Server

    Kikuchi, Mitsuru

    2011-01-01

    Frontiers in Fusion Research provides a systematic overview of the latest physical principles of fusion and plasma confinement. It is primarily devoted to the principle of magnetic plasma confinement, that has been systematized through 50 years of fusion research. Frontiers in Fusion Research begins with an introduction to the study of plasma, discussing the astronomical birth of hydrogen energy and the beginnings of human attempts to harness the Sun's energy for use on Earth. It moves on to chapters that cover a variety of topics such as: * charged particle motion, * plasma kinetic theory, *

  11. Fusion reactor safety

    International Nuclear Information System (INIS)

    Nuclear fusion could soon become a viable energy source. Work in plasma physics, fusion technology and fusion safety is progressing rapidly in a number of Member States and international collaboration continues on work aiming at the demonstration of fusion power generation. Safety of fusion reactors and technological and radiological aspects of waste management are important aspects in the development and design of fusion machines. In order to provide an international forum to review and discuss the status and the progress made since 1983 in programmes related to operational safety aspects of fusion reactors, their waste management and decommissioning concepts, the IAEA had organized the Technical Committee on ''Fusion Reactor Safety'' in Culham, 3-7 November 1986. All presentations of this meeting were divided into four sessions: 1. Statements on National-International Fusion Safety Programmes (5 papers); 2. Operation and System Safety (15 papers); 3. Waste Management and Decommissioning (5 papers); 4. Environmental Impacts (6 papers). A separate abstract was prepared for each of these 31 papers. Refs, figs, tabs

  12. Laser fusion program overview

    International Nuclear Information System (INIS)

    This program is structured to proceed through a series of well defined fusion milestones to proof of the scientific feasibility, of laser fusion with the Shiva Nova system. Concurrently, those key technical areas, such as advanced lasers, which are required to progress beyond proof of feasibility, are being studied. We have identified and quantified the opportunities and key technical issues in military applications, such as weapons effects simulations, and in civilian applications, such as central-station electric power production. We summarize the current status and future plans for the laser fusion program at LLL, emphasizing the civilian applications of laser fusion

  13. Magnetic-confinement fusion

    Science.gov (United States)

    Ongena, J.; Koch, R.; Wolf, R.; Zohm, H.

    2016-05-01

    Our modern society requires environmentally friendly solutions for energy production. Energy can be released not only from the fission of heavy nuclei but also from the fusion of light nuclei. Nuclear fusion is an important option for a clean and safe solution for our long-term energy needs. The extremely high temperatures required for the fusion reaction are routinely realized in several magnetic-fusion machines. Since the early 1990s, up to 16 MW of fusion power has been released in pulses of a few seconds, corresponding to a power multiplication close to break-even. Our understanding of the very complex behaviour of a magnetized plasma at temperatures between 150 and 200 million °C surrounded by cold walls has also advanced substantially. This steady progress has resulted in the construction of ITER, a fusion device with a planned fusion power output of 500 MW in pulses of 400 s. ITER should provide answers to remaining important questions on the integration of physics and technology, through a full-size demonstration of a tenfold power multiplication, and on nuclear safety aspects. Here we review the basic physics underlying magnetic fusion: past achievements, present efforts and the prospects for future production of electrical energy. We also discuss questions related to the safety, waste management and decommissioning of a future fusion power plant.

  14. On fusion for multispectral iris recognition

    OpenAIRE

    Wild, Peter; Radu, Petru; Ferryman, James

    2015-01-01

    Multispectral iris recognition uses information from multiple bands of the electromagnetic spectrum to better represent certain physiological characteristics of the iris texture and enhance obtained recognition accuracy. This paper addresses the questions of single versus cross spectral performance and compares score-level fusion accuracy for different feature types, combining different wavelengths to overcome limitations in less constrained recording environments. Further it is investigated ...

  15. Membrane fusion machines of paramyxoviruses: capture of intermediates of fusion

    OpenAIRE

    Charles J Russell; Theodore S Jardetzky; Lamb, Robert A.

    2001-01-01

    Peptides derived from heptad repeat regions adjacent to the fusion peptide and transmembrane domains of many viral fusion proteins form stable helical bundles and inhibit fusion specifically. Paramyxovirus SV5 fusion (F) protein-mediated fusion and its inhibition by the peptides N-1 and C-1 were analyzed. The temperature dependence of fusion by F suggests that thermal energy, destabilizing proline residues and receptor binding by the hemagglutinin–neuraminidase (HN) protein collectively contr...

  16. Cognitive fusion analysis based on context

    Science.gov (United States)

    Blasch, Erik P.; Plano, Susan

    2004-04-01

    The standard fusion model includes active and passive user interaction in level 5 - "User Refinement". User refinement is more than just details of passive automation partitioning - it is the active management of information. While a fusion system can explore many operational conditions over myopic changes, the user has the ability to reason about the hyperopic "big picture." Blasch and Plano developed cognitive-fusion models that address user constraints including: intent, attention, trust, workload, and throughput to facilitate hyperopic analysis. To enhance user-fusion performance modeling (i.e. confidence, timeliness, and accuracy); we seek to explore the nature of context. Context, the interrelated conditions of which something exists, can be modeled in many ways including geographic, sensor, object, and environmental conditioning. This paper highlights user refinement actions based on context to constrain the fusion analysis for accurately representing the trade space in the real world. As an example, we explore a target identification task in which contextual information from the user"s cognitive model is imparted to a fusion belief filter.

  17. Nuclear fusion inside condense matters

    Institute of Scientific and Technical Information of China (English)

    HE Jing-tang

    2007-01-01

    This article describes in detail the nuclear fusion inside condense matters--the Fleischmann-Pons effect, the reproducibility of cold fusions, self-consistentcy of cold fusions and the possible applications.

  18. Relationship Between the Private Sector and Fusion Centers: Potential Causes for Concern and Realities

    OpenAIRE

    O'Neil, Siobhan

    2008-01-01

    This article appeared in Homeland Security Affairs (April 2008), Supplement no.2 The emergence of fusion centers has been met with much anticipation, as well as feelings ranging from relief to fear. Much of the latter has focused on the potential for fusion centers to disregard civil liberties, a position promoted by the American Civil Liberties Union (ACLU). In a 2007 report entitled, What's Wrong With Fusion Centers, the ACLU argued enhancing private sector integration with fusion center...

  19. Fusion hindrance and roles of shell effects in superheavy mass region

    OpenAIRE

    Aritomo, Y.

    2006-01-01

    We present the first attempt of systematically investigating the effects of shell correction energy for a dynamical process, which includes fusion, fusion-fission and quasi-fission processes. In the superheavy mass region, for the fusion process, shell correction energy plays a very important role and enhances the fusion probability when the colliding partner has a strong shell structure. By analyzing the trajectory in three-dimensional coordinate space with the Langevin equation, we reveal t...

  20. The Duals of Fusion Frames for Experimental Data Transmission Coding of High Energy Physics

    OpenAIRE

    Jinsong Leng; Qixun Guo; Tingzhu Huang

    2013-01-01

    The experimental data transmission is an important part of high energy physics experiment. In this paper, we connect fusion frames with the experimental data transmission implement of high energy physics. And we research the utilization of fusion frames for data transmission coding which can enhance the transmission efficiency, robust against erasures, and so forth. For this application, we first characterize a class of alternate fusion frames which are duals of a given fusion frame in a Hilb...

  1. Fusion Hindrance and the Role of Shell Effects in the Superheavy Mass Region

    OpenAIRE

    Aritomo, Y.

    2006-01-01

    We present the first attempt of the systematical investigation about the effects of shell correction energy for dynamical processes, which include fusion, fusion-fission and quasi-fission processes. In the superheavy mass region, for the fusion process, the shell correction energy plays a very important role and enhances the fusion probability, when the colliding partner has strong shell structure. By analyzing the trajectory in the three-dimensional coordinate space with a Langevin equation,...

  2. Tight p-fusion frames

    OpenAIRE

    Bachoc, Christine; Ehler, Martin

    2012-01-01

    Fusion frames enable signal decompositions into weighted linear subspace components. For positive integers p, we introduce p-fusion frames, a sharpening of the notion of fusion frames. Tight p-fusion frames are closely related to the classical notions of designs and cubature formulas in Grassmann spaces and are analyzed with methods from harmonic analysis in the Grassmannians. We define the p-fusion frame potential, derive bounds for its value, and discuss the connections to tight p-fusion fr...

  3. Fusion of biological membranes

    Indian Academy of Sciences (India)

    K Katsov; M Müller; M Schick

    2005-06-01

    The process of membrane fusion has been examined by Monte Carlo simulation, and is found to be very different than the conventional picture. The differences in mechanism lead to several predictions, in particular that fusion is accompanied by transient leakage. This prediction has recently been verified. Self-consistent field theory is applied to examine the free energy barriers in the different scenarios.

  4. Two Horizons of Fusion

    Science.gov (United States)

    Lo, Mun Ling; Chik, Pakey Pui Man

    2016-01-01

    In this paper, we aim to differentiate the internal and external horizons of "fusion." "Fusion" in the internal horizon relates to the structure and meaning of the object of learning as experienced by the learner. It clarifies the interrelationships among an object's critical features and aspects. It also illuminates the…

  5. The fusion reactor

    International Nuclear Information System (INIS)

    Basic principles of the fusion reactor are outlined. Plasma heating and confinement schemes are described. These confinement systems include the linear Z pinch, magnetic mirrors and Tokamaks. A fusion reactor is described and a discussion is given of its environmental impact and its fuel situation. (R.L.)

  6. Fusion Canada issue 4

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program. Included in this issue is a technical update on Tokamak de Varennes, a report on the Beatrix II Breeding Materials Test Program, the Tritium glovebox system for UPM, Saudi Arabia, a broad update of the Canadian Fusion Fuels Technology Project is also included. 1 fig

  7. Fusion Canada issue 14

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program. Included in this issue is a report on a fusion cooperation agreement between Japan and Canada, an update at Tokamak de Varennes on plasma biasing experiments and boronization tests and a collaboration between Canada and the U.S. on a compact toroid fuelling gun. 4 figs

  8. Nuclear fusion in Jupiter

    International Nuclear Information System (INIS)

    We study nuclear fusion occurring according to conventional wisdom in the planet Jupiter. In particular, we consider if in a standard evolutionary model of Jupiter a significant part of Jupiter's luminosity has been due to nuclear fusion at any time during its evolution. Nuclear rate equations in dense matter allowing for screening and pressure effects have been integrated in time

  9. FUSION03, Concluding Remarks

    OpenAIRE

    Balantekin, A. B.

    2004-01-01

    Fusion reactions below the Coulomb barrier provide new insights into multidimensional quantum tunneling, nuclear reaction dynamics and nuclear structure. These reactions are also of considerable interest to nuclear astrophysics. In this summary recent developments in the field are reviewed and open questions related to subbarrier fusion are presented.

  10. Controlled thermonuclear fusion

    CERN Document Server

    Bobin, Jean Louis

    2014-01-01

    The book is a presentation of the basic principles and main achievements in the field of nuclear fusion. It encompasses both magnetic and inertial confinements plus a few exotic mechanisms for nuclear fusion. The state-of-the-art regarding thermonuclear reactions, hot plasmas, tokamaks, laser-driven compression and future reactors is given.

  11. Fusion reactor materials

    International Nuclear Information System (INIS)

    At the Belgian Nuclear Research Centre SCK-CEN, activities related to fusion focus on environmental tolerance of opto-electronic components. The objective of this program is to contribute to the knowledge on the behaviour, during and after neutron irradiation, of fusion-reactor materials and components. The main scientific activities for 1997 are summarized

  12. Fusion Canada issue 15

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program. Included in this issue is a report on the 1996 IAEA Fusion Conference site, operations at the Tokamak de Varennes including divertor pumping of impurities and pumping of carbon monoxide and methane, a discussion of the CFFTP and it's role. 1 fig

  13. Fusion product spectra

    International Nuclear Information System (INIS)

    Accurate fusion product data is required for most fusion plasma simulations. The energy broadening of reaction products is demonstrated to be more complicated than the usual Gaussian broadening. The accurate integrals are performed to obtain , , and for all binary reactions in the four- and five-nucleon systems. Reaction cross sections were developed using R-Matrix models that include most recent measurements

  14. Magnetic fusion theory effort

    International Nuclear Information System (INIS)

    The present publication is a comprehensive listing of the magnetic fusion theory effort. It updates the last publication, ERDA 77-18, and gives data on the FY 1977 and FY 1978 budgets. There is a section devoted to the National Magnetic Fusion Computer Center

  15. Fusion Canada issue 22

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program highlighting in this issue a bi-lateral meeting between Canada and Japan, water and hydrogen detritiation, in-situ tokamak surface analysis, an update of CCFM/TdeV and tritium accounting Industry guidance in Fusion, fast probe for plasma-surface interaction. 4 figs

  16. Magnetic Fusion Program Plan

    International Nuclear Information System (INIS)

    This Plan reflects the present conditions of the energy situation and is consistent with national priorities for the support of basic and applied research. It is realistic in taking advantage of the technical position that the United States has already established in fusion research to make cost-effective progress toward the development of fusion power as a future energy option

  17. Fusion reactor studies

    International Nuclear Information System (INIS)

    A review is given of fusion reactor systems studies, the objectives of these studies are outlined and some recent conceptual reactor designs are described. The need for further studies in greater depth is indicated so that progress towards a commercial fusion reactor may be consolidated. (U.K.)

  18. Magnetic Fusion Energy

    International Nuclear Information System (INIS)

    The industrialized countries most active in nuclear fusion (China, Korea, Japan, India, Russia, the United States and the European Union) are participating in the development of the ITER, an experimental reactor representing a fundamental step toward nuclear fusion energy. Europe, and Italy in particular, are deeply involved in this initiative with an important related project too.

  19. Cold fusion stays elusive

    International Nuclear Information System (INIS)

    A short overview of papers presented at the 6. international conference on cold fusion which has taken place 13-18 Oct 1996 in Hokkaido (Japan) is given. The typical electrolysis cell used for cold fusion experiments is recalled and some new results are described, like a correlation of helium 4 production and heat excess. (C.B.)

  20. Fusion Canada issue 19

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program. Included in this issue is a report on the IAEA Plasma Biasing Meeting, the new IEA program -Nuclear Technology of Fusion reactors, TFTR tritium purification system, an update by CCFM on machine additions and modifications, and news of a new compact Toroid injector at the University of Saskatchewan. 1 fig

  1. Fusion Power Deployment

    International Nuclear Information System (INIS)

    Fusion power plants could be part of a future portfolio of non-carbon dioxide producing energy supplies such as wind, solar, biomass, advanced fission power, and fossil energy with carbon dioxide sequestration. In this paper, we discuss key issues that could impact fusion energy deployment during the last half of this century. These include geographic issues such as resource availability, scale issues, energy storage requirements, and waste issues. The resource needs and waste production associated with fusion deployment in the U.S. should not pose serious problems. One important feature of fusion power is the fact that a fusion power plant should be locatable within most local or regional electrical distribution systems. For this reason, fusion power plants should not increase the burden of long distance power transmission to our distribution system. In contrast to fusion power, regional factors could play an important role in the deployment of renewable resources such as wind, solar and biomass or fossil energy with CO2 sequestration. We examine the role of these regional factors and their implications for fusion power deployment

  2. Controlled Nuclear Fusion.

    Science.gov (United States)

    Glasstone, Samuel

    This publication is one of a series of information booklets for the general public published by The United States Atomic Energy Commission. Among the topics discussed are: Importance of Fusion Energy; Conditions for Nuclear Fusion; Thermonuclear Reactions in Plasmas; Plasma Confinement by Magnetic Fields; Experiments With Plasmas; High-Temperature…

  3. Filter Bank Fusion Frames

    CERN Document Server

    Chebira, Amina; Mixon, Dustin G

    2010-01-01

    In this paper we characterize and construct novel oversampled filter banks implementing fusion frames. A fusion frame is a sequence of orthogonal projection operators whose sum can be inverted in a numerically stable way. When properly designed, fusion frames can provide redundant encodings of signals which are optimally robust against certain types of noise and erasures. However, up to this point, few implementable constructions of such frames were known; we show how to construct them using oversampled filter banks. In this work, we first provide polyphase domain characterizations of filter bank fusion frames. We then use these characterizations to construct filter bank fusion frame versions of discrete wavelet and Gabor transforms, emphasizing those specific finite impulse response filters whose frequency responses are well-behaved.

  4. Compact fusion reactors

    CERN Document Server

    CERN. Geneva

    2015-01-01

    Fusion research is currently to a large extent focused on tokamak (ITER) and inertial confinement (NIF) research. In addition to these large international or national efforts there are private companies performing fusion research using much smaller devices than ITER or NIF. The attempt to achieve fusion energy production through relatively small and compact devices compared to tokamaks decreases the costs and building time of the reactors and this has allowed some private companies to enter the field, like EMC2, General Fusion, Helion Energy, Lawrenceville Plasma Physics and Lockheed Martin. Some of these companies are trying to demonstrate net energy production within the next few years. If they are successful their next step is to attempt to commercialize their technology. In this presentation an overview of compact fusion reactor concepts is given.

  5. FUSION OF MEDICAL IMAGES

    Directory of Open Access Journals (Sweden)

    ALINE APARECIDA DE OLIVEIRA

    2013-08-01

    Full Text Available The use of image multiple modalities to achieve medical diagnosis has been commom practice lately. Nowadays the most used practice is medical image fusion, that is integrating information from several different methods within the same image. This paper aims at showing aplication and functionality of medical image fusion process such as Computed Tomography, Magnetic Resonance Imaging, Positron Emission Tomography and Doppler U.S. Image fusion process can be perfomed by pixel to pixel, region to region as well as based on decision taking. Free softwares can be found in the internet and images can be obtained either in separated or conneceted equipments. The choice of processes depends on several factors and the purpose of fusion as well as characteristics and conditions of each method should be taken into consideration. Currently equipment manufacturers are investing at improving the quality and detection capacity of images aiming at upgrading the fusion process which makes image interpretation more evident and trustworthy.

  6. Some fusion perspectives

    International Nuclear Information System (INIS)

    Some of the concepts of nuclear fusion reactions, advanced fusion fuels, environmental impacts, etc., are explored using the following general outline: I. Principles of Fusion (Nuclear Fuels and Reactions, Lawson Condition, n tau vs T, Nuclear Burn Characteristics); II. Magnetic Mirror Possibilities (the Ion Layer and Electron Layer, Exponential Build-up at MeV energies, Lorentz trapping at GeV energies); III. Pellet Fuel Fusion Prospects (Advanced Pellet Fuel Fusion Prospects, Burn Characteristics and Applications, Excitation-heating Prospects for Runaway Ion Temperatures). Inasmuch as the outline is very skeletal, a significant research and development effort may be in order to evaluate these prospects in more detail and hopefully ''harness the H-bomb'' for peaceful applications, the author concludes. 28 references

  7. Decomposition of incomplete fusion

    International Nuclear Information System (INIS)

    The velocity distribution of fusion-like products formed in the reaction 701 MeV 28Si+100Mo is decomposed into 26 incomplete fusion channels. The momentum deficit of the residue per nonevaporative mass unit is approximately equal to the beam momentum per nucleon. The yields of the incomplete fusion channels correlate with the Q-value for projectile fragmentation rather than that for incomplete fusion. The backward angle multiplicities of light particles and heavy ions increase with momentum transfer, however, the heavy ion multiplicities also depend on the extent of the fragmentation of the incomplete fusion channel. These data indicate that at fixed linear momentum transfer, increased fragmentation of the unfused component is related to a reduced transferred angular momentum. 22 refs., 6 figs., 1 tab

  8. Towards fusion power

    International Nuclear Information System (INIS)

    An attempt has been made to present general but broad review of the recent developments in the field of plasma physics and its application to fusion power. The first chapter describes the fusion reactions and fusion power systems. The second chapter deals in detail with production and behaviour of plasma, screening, oscillations, instability, energy losses, temperature effects, etc. Magnetic confinements, including pinch systems, toroidal systems such as Tokamac and stellarator, minor machine, etc. are discussed in detail in chapter III. Laser produced plasma, laser implosion and problems associated with it and future prospects are explained in chapter IV. Chapter V is devoted entirely to the various aspects of hybrid systems. The last chapter throws light on problems of fusion technology, such as plasma heating, vacuum requirements, radiation damage, choice of materials, blanket problems, hazards of fusion reactions, etc. (K.B.)

  9. Industry's role in inertial fusion

    International Nuclear Information System (INIS)

    This paper is an address to the Tenth Symposium on Fusion Engineering. The speaker first addressed the subject of industry's role in inertial fusion three years earlier in 1980, outlining programs that included participation in the Shiva construction project, and the industrial participants' program set up in the laser fusion program to bring industrial scientists and engineers into the laboratory to work on laser fusion. The speaker is now the president of KMS Fusion, Inc., the primary industrial participant in the inertial fusion program. The outlook for fusion energy and the attitude of the federal government toward the fusion program is discussed

  10. RS Image Fusion Technique for Information Preservation

    Institute of Scientific and Technical Information of China (English)

    WU Lianxi; SU Xiaoxia; LI Dajun

    2004-01-01

    A simple spectral preserving image fusion technique, Edge Enhancement Color Normalized (EECN), was proposed to merge two kinds of image data. In addition, a mathematical model was also proposed to evaluate spectral property of the fused production of EECN. The results were clearly demonstrated by an image fusion experiment using Landsat-5 TM and IRS-1C Panchromatic images of Beijing, China. The visual evaluation and mathematical analysis compared with Brovey transform confirmed that the fused image of EECN is quite similar in color to the lower resolution multi-spectral images, and its space resolution is the same as the higher solution panchromatic image.

  11. Fusion Frames: Existence and Construction

    CERN Document Server

    Calderbank, Robert; Heinecke, Andreas; Kutyniok, Gitta; Pezeshki, Ali

    2009-01-01

    Fusion frame theory is an emerging mathematical theory that provides a natural framework for performing hierarchical data processing. A fusion frame is a frame-like collection of subspaces in a Hilbert space, thereby generalizing the concept of a frame for signal representation. In this paper, we study the existence and construction of fusion frames. We first present a complete characterization of a special class of fusion frames, called Parseval fusion frames. The value of Parseval fusion frames is that the inverse fusion frame operator is equal to the identity and therefore signal reconstruction can be performed with minimal complexity. We then introduce two general methods -- the spatial complement and the Naimark complement -- for constructing a new fusion frame from a given fusion frame. We then establish existence conditions for fusion frames with desired properties. In particular, we address the following question: Given $M, N, m \\in \\NN$ and $\\{\\lambda_j\\}_{j=1}^M$, does there exist a fusion frame in ...

  12. Scenarios for fusion energy

    International Nuclear Information System (INIS)

    Are there scenarios for the near future (5-10 years) in which the public interest in, and political support for, fusion energy are likely to increase significantly? If such a scenario should occur, what should the status of heavy-ion fusion be at that time so that it is looked upon as a favored approach? If such a status can be identified, what should the research strategy be so that this status can be achieved in a timely way? This paper will look for answers to the first two of the above questions. If the goal of a desired status can be identified, the research strategy may become obvious. Of the four long-term energy options - coal, solar, fission breeders and fusion - the present position of fusion is in fourth place, never having generated any power. However, coal has environmental and safety problems. Solar has limitations of cost, availability and space requirements. Fission in general, and breeder reactors in particular, have problems of cost, environment and public acceptance. To be accepted and elevated to a position of equality with the others, heavy-ion fusion must simultaneously avoid their real and perceived major flaws. To succeed, heavy-ion fusion must strive to be markedly the best fusion option. (orig.)

  13. Fusion Studies in Japan

    Science.gov (United States)

    Ogawa, Yuichi

    2016-05-01

    A new strategic energy plan decided by the Japanese Cabinet in 2014 strongly supports the steady promotion of nuclear fusion development activities, including the ITER project and the Broader Approach activities from the long-term viewpoint. Atomic Energy Commission (AEC) in Japan formulated the Third Phase Basic Program so as to promote an experimental fusion reactor project. In 2005 AEC has reviewed this Program, and discussed on selection and concentration among many projects of fusion reactor development. In addition to the promotion of ITER project, advanced tokamak research by JT-60SA, helical plasma experiment by LHD, FIREX project in laser fusion research and fusion engineering by IFMIF were highly prioritized. Although the basic concept is quite different between tokamak, helical and laser fusion researches, there exist a lot of common features such as plasma physics on 3-D magnetic geometry, high power heat load on plasma facing component and so on. Therefore, a synergetic scenario on fusion reactor development among various plasma confinement concepts would be important.

  14. Fusion research in Hungary

    International Nuclear Information System (INIS)

    Hungarian fusion research started in the 1970s, when the idea of installing a small tokamak experiment emerged. In return to computer equipment a soviet tokamak was indeed sent to Hungary and started to operate as MT-1 at the Central Research Institute for Physics (KFKI) in 1979. Major research topics included diagnostic development, edge plasma studies and investigation of disruptions. Following a major upgrade in 1992 (new vacuum vessel, active position control and PC network based data acquisition system) the MT-1M tokamak was used for the study of transport processes with trace impurity injection, micropellet ablation studies, X-ray tomography and laser blow-off diagnostic development. Although funding ceased in the middle of the 90's the group was held alive by collaborations with EU fusion labs: FZ -Juelich, IPP-Garching and CRPP-EPFL Lausanne. In 1998 the machine was dismantled due to reorganization of the Hungarian Academy of Sciences. New horizons opened to fusion research from 1999, when Hungary joined EURATOM and a fusion Association was formed. Since then fusion physics studies are done in collaboration with major EU fusion laboratories, Hungarian researchers also play an active role in JET diagnostics upgrade and ITER design. Major topics are pellet ablation studies, plasma turbulence diagnosis using Beam Emission Spectroscopy and other techniques, tomography and plasma diagnostics using various neutral beams. In fusion relevant technology R and D Hungary has less records. Before joining EURATOM some materials irradiation studies were done at the Budapest Research Reactor at KFKI-AEKI. The present day fusion technology programme focuses still on irradiation studies, nuclear material database and electromagnetic testing techniques. Increasing the fusion technology research activities is a difficult task, as the competition in Hungarian industry is very strong and the interest of organizations in long-term investments into R and D is rather weak and

  15. RSV Fusion: Time for a New Model

    Directory of Open Access Journals (Sweden)

    Richard G. Hegele

    2013-03-01

    Full Text Available In this review we propose a partially hypothetical model of respiratory syncytial virus (RSV binding and entry to the cell that includes the recently discovered RSV receptor nucleolin, in an attempt to stimulate further inquiry in this research area. RSV binding and entry is likely to be a two-step process, the first involving the attachment of the virus to the cell membrane, which may be enhanced by electrostatic interactions with cellular glycoproteins/heparin and the viral G protein, and the second involving fusion to the cell membrane mediated by the viral F protein and a specific cellular fusion receptor. With our recent discovery of nucleolin as a functional fusion receptor for RSV, comes the possibility of a number of new approaches to the development of novel strategies for RSV prophylaxis and therapy, as well as raising some new questions concerning the pathobiology of RSV infection and tropism.

  16. Whither fusion research?

    International Nuclear Information System (INIS)

    The author maintains that the magnetic fusion program is ready for a dramatic change of course. The author's criteria for a desirable fusion power reactor are: it must produce power at a competitive price; it must work with high reliability and maintenance must be easy, fast and low cost, and that the fusion reactor must be safe and not damage the environment. The author's feelings are based on years of observation of the utility industry in general and commercial nuclear power development in particular. The author's views on the undesirability of tokamaks are discussed. Two concepts discussed as alternatives are the Reversed Field Pinch (RFP) and the spheromak

  17. Remote sensing image fusion

    CERN Document Server

    Alparone, Luciano; Baronti, Stefano; Garzelli, Andrea

    2015-01-01

    A synthesis of more than ten years of experience, Remote Sensing Image Fusion covers methods specifically designed for remote sensing imagery. The authors supply a comprehensive classification system and rigorous mathematical description of advanced and state-of-the-art methods for pansharpening of multispectral images, fusion of hyperspectral and panchromatic images, and fusion of data from heterogeneous sensors such as optical and synthetic aperture radar (SAR) images and integration of thermal and visible/near-infrared images. They also explore new trends of signal/image processing, such as

  18. Mirror fusion reactors

    International Nuclear Information System (INIS)

    Conceptual design studies were made of fusion reactors based on the three current mirror-confinement concepts: the standard mirror, the tandem mirror, and the field-reversed mirror. Recent studies of the standard mirror have emphasized its potential as a fusion-fission hybrid reactor, designed to produce fuel for fission reactors. We have designed a large commercial hybrid and a small pilot-plant hybrid based on standard mirror confinement. Tandem mirror designs include a commercial 1000-MWe fusion power plant and a nearer term tandem mirror hybrid. Field-reversed mirror designs include a multicell commercial reactor producing 75 MWe and a single-cell pilot plant

  19. Fusion Reactor Materials

    International Nuclear Information System (INIS)

    The objective of SCK-CEN's programme on fusion reactor materials is to contribute to the knowledge on the behaviour of fusion reactor materials and components during and after irradiation. Ongoing projects include: the study of the mechanical behaviour of structural materials under neutron irradiation; the investigation of the characteristics of irradiated first wall material such as beryllium; the detection of abrupt electrical degradation of insulating ceramics under high temperature and neutron irradiation; and the study of dismantling and waste disposal strategy for fusion reactors. Progress and achievements in these areas in 2000 are discussed

  20. Fusion Reactor Materials

    International Nuclear Information System (INIS)

    The objective of SCK-CEN's programme on fusion reactor materials is to contribute to the knowledge on the radiation-induced behaviour of fusion reactor materials and components as well as to help the international community in building the scientific and technical basis needed for the construction of the future reactor. Ongoing projects include: the study of the mechanical and chemical (corrosion) behaviour of structural materials under neutron irradiation and water coolant environment; the investigation of the characteristics of irradiated first wall material such as beryllium; investigations on the management of materials resulting from the dismantling of fusion reactors including waste disposal. Progress and achievements in these areas in 2001 are discussed

  1. Observation of incomplete fusion reactions at l < l {sub crit}

    Energy Technology Data Exchange (ETDEWEB)

    Yadav, Abhishek, E-mail: abhishekyadav117@gmail.com; Sharma, Vijay R., E-mail: abhishekyadav117@gmail.com; Singh, Devendra P., E-mail: abhishekyadav117@gmail.com; Unnati,; Singh, B. P.; Prasad, R. [Department of Physics, Aligarh Muslim University, Aligarh (UP) - 202 002 (India); Singh, Pushpendra P. [GSI-Helmholtz Centre for Heavy Ion Research GmbH, D-64291 Darmstadt (Germany); Bala, Indu; Kumar, R.; Muralithar, S.; Singh, R. P. [NP-Group: Inter-University Accelerator Center, Aruna Asaf Ali Marg, New Delhi - 110 067 (India); Sharma, M. K. [Department of Physics, S. V. College, Aligarh- 202 001 (India)

    2014-08-14

    In order to understand the presence of incomplete fusion at low energies i.e. 4-7MeV/nucleon and also to study its dependence on various entrance-channel parameters, the two type of measurements (i) excitation function for {sup 12}C+{sup 159}Tb, and (ii) forward recoil ranges for {sup 12}C+{sup 159}Tb systems have been performed. The experimentally measured excitation functions have been analyzed within the framework of compound nucleus decay using statistical model code PACE4. Analysis of data suggests the production of xn/px)n-channels via complete fusion, as these are found to be well reproduced by PACE4 predictions, while, a significant enhancement in the excitation functions of α-emitting channels has been observed over the theoretical ones, which has been attributed due to the incomplete fusion processes. Further, the incomplete fusion events observed in case of forward recoil range measurements have been explained on the basis of the breakup fusion model, where these events may be attributed to the fusion of {sup 8}Be and/or {sup 4}He from {sup 12}C projectile to the target nucleus. In the present work, the SUMRULE model calculations are found to highly underestimate the observed incomplete fusion cross-sections which indicate that the l-values lower than l {sub crit} (limit of complete fusion) significantly contribute to the incomplete fusion reactions.

  2. Inertial thermonuclear fusion by laser

    International Nuclear Information System (INIS)

    The principles of deuterium tritium (DT) magnetic or inertial thermonuclear fusion are given. Even if results would be better with heavy ions beams, most of the results on fusion are obtained with laser beams. Technical and theoretical aspects of the laser fusion are presented with an extrapolation to the future fusion reactor. (A.B.). 34 refs., 17 figs

  3. Fusion Systems for Profinite Groups

    OpenAIRE

    Stancu, Radu; Symonds, Peter

    2012-01-01

    We introduce the notion of a pro-fusion system on a pro-p group, which generalizes the notion of a fusion system on a finite p-group. We also prove a version of Alperin's Fusion Theorem for pro-fusion systems.

  4. Estimation of Total Fusion Reactivity and Contribution from Suprathermal Tail using 3-parameter Dagum Ion Speed Distribution

    CERN Document Server

    Majumdar, Rudrodip

    2016-01-01

    Thermonuclear fusion reactivity is a pivotal quantity in the studies pertaining to fusion energy production, fusion ignition and energy break-even analysis in both inertially and magnetically confined systems. Although nuclear fusion reactivity and thereafter the power density of a magnetic confinement fusion reactor and the fulfillment of the ignition criterion are quantitatively determined by assuming the ion speed distribution to be Maxwellian, a significant population of suprathermal ions,with energy greater than the quasi-Maxwellian background plasma temperature, is generated by the fusion reactions and auxiliary heating in the fusion devices. In the current work 3-parameter Dagum speed distribution has been introduced to include the effect of suprathermal ion population in the calculation of total fusion reactivity. The extent of enhancement in the fusion reactivity, at different back-ground temperatures of the fusion fuel plasma, due to the suprathermal ion population has also been discussed.

  5. Reaction mechanisms in heavy ion fusion

    Directory of Open Access Journals (Sweden)

    Lubian J.

    2011-10-01

    Full Text Available We discuss the reaction mechanisms involved in heavy ion fusion. We begin with collisions of tightly bound systems, considering three energy regimes: energies above the Coulomb barrier, energies just below the barrier and deep sub-barrier energies. We show that channel coupling effects may influence the fusion process at above-barrier energies, increasing or reducing the cross section predicted by single barrier penetration model. Below the Coulomb barrier, it enhances the cross section, and this effect increases with the system’s size. It is argued that this behavior can be traced back to the increasing importance of Coulomb coupling with the charge of the collision partners. The sharp drop of the fusion cross section observed at deep sub-barrier energies is addressed and the theoretical approaches to this phenomenon are discussed. We then consider the reaction mechanisms involved in fusion reactions of weakly bound systems, paying particular attention to the calculations of complete and incomplete fusion available in the literature.

  6. Improved magnetic resonance myelography using image fusion

    International Nuclear Information System (INIS)

    To demonstrate that the disadvantage of missing anatomical information in heavily T2-weighted MR myelography images can be eliminated by image fusion and phase encoding in the coronal direction of the source images, resulting in MR myelography images comparable to the gold standard, i. e., post-myelography CT. This study included 110 patients suffering from extradural pathologies of the cervical and lumbar spine. All patients were investigated using 3D MR myelography and post-myelography CT. The MRI data were post-processed using image fusion and reconstruction algorithms and were compared to the corresponding images of post-myelography CT. Our approach for visualization (3D MR myelography) was able to depict intradural structures in high spatial resolution and without artifacts. The results of our visualization approach were comparable to the gold standard - post-myelography CT. Anatomical correlation was reached by image fusion of different MR data sets. The required post-processing steps were performed quickly and were available on a commercial workstation. Image fusion of different MR data sets allows for visualization of 3D data sets with enhanced quality. The results for the visualization of MR myelography in particular are comparable to conventional myelography and post-myelography CT. The missing anatomical information in heavily T2-weighted MR myelography images can be compensated by image fusion with conventional MRI. (orig.)

  7. Slantlet Transform for Multispectral Image Fusion

    Directory of Open Access Journals (Sweden)

    Adnan H.M. Al-Helali

    2009-01-01

    Full Text Available Problem statement: Image fusion is a process by which multispectral and panchromatic images, or some of their features, are combined together to form a high spatial/high spectral resolutions image. The successful fusion of images acquired from different modalities or instruments is a great importance issue in remote sensing applications. Approach: A new method of image fusion was introduced. It was based on a hybrid transform, which is an extension of Ridgelet transform. It used the slantlet transform instead of wavelet transform in the final steps of Ridgelet transform. The slantlet transform was an orthogonal discrete wavelet transform with two zero moments and with improved time localization. Results: Since edges and noise played fundamental role in image understanding, this hybrid transform was proved to be good way to enhance the edges and reduce the noise. Conclusion: The proposed method of fusion presented richer information in spatial and spectral domains simultaneously as well as it had reached an optimum fusion result.

  8. Fusion reactor materials

    International Nuclear Information System (INIS)

    This is the fifteenth in a series of semiannual technical progress reports on fusion reactor materials. This report combines research and development activities which were previously reported separately in the following progress reports: Alloy Development for Irradiation Performance; Damage Analysis and Fundamental Studies; Special purpose Materials. These activities are concerned principally with the effects of the neutronic and chemical environment on the properties and performance of reactor materials; together they form one element of the overall materials programs being conducted in support of the Magnetic Fusion Energy Program of the U.S. Department of Energy. The Fusion Reactor Materials Program is a national effort involving several national laboratories, universities, and industries. The purpose of this series of reports is to provide a working technical record for the use of the program participants, and to provide a means of communicating the efforts of materials scientists to the rest of the fusion community, both nationally and worldwide

  9. Fusion technology programme

    International Nuclear Information System (INIS)

    KfK participates to the Fusion Technology Programme of the European Community. Most of the work in progress addresses the Next European Torus (NET) and the long term technology aspects as defined in the 82/86 programme. A minor part serves to preparation of future contributions and to design studies on fusion concepts in a wider perspective. The Fusion Technology Programme of Euratom covers mainly aspects of nuclear engineering. Plasma engineering, heating, refueling and vacuum technology are at present part of the Physics Programme. In view of NET, integration of the different areas of work will be mandatory. KfK is therefore prepared to address technical aspects beyond the actual scope of the physics experiments. The technology tasks are reported project wise under title and code of the Euratom programme. Most of the projects described here are shared with other European fusion laboratories as indicated in the table annexed to this report. (orig./GG)

  10. Fusion-breeder program

    International Nuclear Information System (INIS)

    The various approaches to a combined fusion-fission reactor for the purpose of breeding 239Pu and 233U are described. Design aspects and cost estimates for fuel production and electricity generation are discussed

  11. Label Fusion Strategy Selection

    Directory of Open Access Journals (Sweden)

    Nicolas Robitaille

    2012-01-01

    Full Text Available Label fusion is used in medical image segmentation to combine several different labels of the same entity into a single discrete label, potentially more accurate, with respect to the exact, sought segmentation, than the best input element. Using simulated data, we compared three existing label fusion techniques—STAPLE, Voting, and Shape-Based Averaging (SBA—and observed that none could be considered superior depending on the dissimilarity between the input elements. We thus developed an empirical, hybrid technique called SVS, which selects the most appropriate technique to apply based on this dissimilarity. We evaluated the label fusion strategies on two- and three-dimensional simulated data and showed that SVS is superior to any of the three existing methods examined. On real data, we used SVS to perform fusions of 10 segmentations of the hippocampus and amygdala in 78 subjects from the ICBM dataset. SVS selected SBA in almost all cases, which was the most appropriate method overall.

  12. Optical Fiber Fusion Splicing

    CERN Document Server

    Yablon, Andrew D

    2005-01-01

    This book is an up-to-date treatment of optical fiber fusion splicing incorporating all the recent innovations in the field. It provides a toolbox of general strategies and specific techniques that the reader can apply when optimizing fusion splices between novel fibers. It specifically addresses considerations important for fusion splicing of contemporary specialty fibers including dispersion compensating fiber, erbium-doped gain fiber, polarization maintaining fiber, and microstructured fiber. Finally, it discusses the future of optical fiber fusion splicing including silica and non-silica based optical fibers as well as the trend toward increasing automation. Whilst serving as a self-contained reference work, abundant citations from the technical literature will enable readers to readily locate primary sources.

  13. Middle interhemispheric fusion

    International Nuclear Information System (INIS)

    We report a case of a 19-year-old woman with seizures and mental deficiency attributed to perinatal anoxia. Brain MR showed a congenital malformation, a rare form of semilobar holoprosencephaly termed middle interhemispheric fusion. (orig.)

  14. Fusion plasma physics

    CERN Document Server

    Stacey, Weston M

    2012-01-01

    This revised and enlarged second edition of the popular textbook and reference contains comprehensive treatments of both the established foundations of magnetic fusion plasma physics and of the newly developing areas of active research. It concludes with a look ahead to fusion power reactors of the future. The well-established topics of fusion plasma physics -- basic plasma phenomena, Coulomb scattering, drifts of charged particles in magnetic and electric fields, plasma confinement by magnetic fields, kinetic and fluid collective plasma theories, plasma equilibria and flux surface geometry, plasma waves and instabilities, classical and neoclassical transport, plasma-materials interactions, radiation, etc. -- are fully developed from first principles through to the computational models employed in modern plasma physics. The new and emerging topics of fusion plasma physics research -- fluctuation-driven plasma transport and gyrokinetic/gyrofluid computational methodology, the physics of the divertor, neutral ...

  15. Incomplete fusion reactions

    International Nuclear Information System (INIS)

    Various aspects of the mechanism of heavy-ion induced reactions in the range of bombarding energies from a few to about 20 MeV/A are reviewed with special emphasis on the reactions for very asymmetric systems. Results of the experimental studies of binary reactions and particularly of the incomplete fusion reactions (selected by means of various coincidence techniques)are discussed. A model of generalized critical angular momentum is formulated. The model explains essential features of the incomplete fusion reactions and predicts that particular reaction channels are localized in well defined regions of angular momenta. An extension of this model (the sum-rule model) is also proposed in attempt to consistently describe the complete fusion reactions, incomplete fusion reactions and multibody reactions in the framework of statistical competition constrained by the angular momentum limitations. (author)

  16. Fusion technology (FT)

    International Nuclear Information System (INIS)

    The annual report of tha fusion technology (FT) working group discusses the projects carried out by the participating institutes in the fields of 1) fuel injection and plasma heating, 2) magnetic field technology, and 3) systems investigations. (HK)

  17. International fusion og spaltning

    DEFF Research Database (Denmark)

    Hansen, Lone L.

    Bogen analyserer de nye muligheder fra 2007 i europæisk ret med hensyn til fusion eller spaltning mellem aktieselskaber og anpartsselskaber med hjemsted i forskellige europæiske lande. Bogen gennemgår de nye muligheder for strukturændringer, der herved er opstået mulighed for, og den sætter fokus...... på det spørgsmål, der i en grænseoverskridende fusion eller spaltning vil blive helt dominerende: Hvilken form for medbestemmelse (medarbejderrepræsentation i bestyrelse mv.) der eventuelt skal være i det fortsættende selskab efter fusionen. Bogen behandler både fusion med et dansk selskab som det...... fortsættende og fusion med et dansk selskab som det ophørende....

  18. Fusion technology development

    International Nuclear Information System (INIS)

    This report includes information on the following chapters: (1) conceptual design studies, (2) magnetics, (3) plasma heating, fueling, and exhaust, (4) materials for fusion reactors, (5) alternate applications, and (6) environment and safety

  19. Cell fusions in mammals

    DEFF Research Database (Denmark)

    Larsson, Lars-Inge; Bjerregaard, Bolette; Talts, Jan Fredrik

    2008-01-01

    Cell fusions are important to fertilization, placentation, development of skeletal muscle and bone, calcium homeostasis and the immune defense system. Additionally, cell fusions participate in tissue repair and may be important to cancer development and progression. A large number of factors appear...... to regulate cell fusions, including receptors and ligands, membrane domain organizing proteins, proteases, signaling molecules and fusogenic proteins forming alpha-helical bundles that bring membranes close together. The syncytin family of proteins represent true fusogens and the founding member......, syncytin-1, has been documented to be involved in fusions between placental trophoblasts, between cancer cells and between cancer cells and host ells. We review the literature with emphasis on the syncytin family and propose that syncytins may represent universal fusogens in primates and rodents, which...

  20. Cold nuclear fusion

    Directory of Open Access Journals (Sweden)

    Huang Zhenqiang Huang Yuxiang

    2013-10-01

    Full Text Available In normal temperature condition, the nuclear force constraint inertial guidance method, realize the combination of deuterium and tritium, helium and lithium... And with a magnetic moment of light nuclei controlled cold nuclear collide fusion, belongs to the nuclear energy research and development in the field of applied technology "cold nuclear collide fusion". According to the similarity of the nuclear force constraint inertial guidance system, the different velocity and energy of the ion beam mixing control, developed ion speed dc transformer, it is cold nuclear fusion collide, issue of motivation and the nuclear power plant start-up fusion and power transfer system of the important equipment, so the merger to apply for a patent

  1. Fusion Revisits CERN

    CERN Multimedia

    2001-01-01

    It's going to be a hot summer at CERN. At least in the Main Building, where from 13 July to 20 August an exhibition is being hosted on nuclear fusion, the energy of the Stars. Nuclear fusion is the engine driving the stars but also a potential source of energy for mankind. The exhibition shows the different nuclear fusion techniques and research carried out on the subject in Europe. Inaugurated at CERN in 1993, following collaboration between Lausanne's CRPP-EPFL and CERN, with input from Alessandro Pascolini of Italy's INFN, this exhibition has travelled round Europe before being revamped and returning to CERN. 'Fusion, Energy of the Stars', from 13 July onwards, Main Building

  2. Fusion research in India

    International Nuclear Information System (INIS)

    The economic growth of our country demands a rapid increase in the energy output. Fusion is one such alternate clean source of energy to contribute in the energy mix towards the second half of the century, with a virtually inexhaustible fuel supply. The environmental impact of fusion would be acceptable and relatively safe. These advantages have driven the world fusion research programme since its inception. Till a pure fusion energy source is available, it is worthwhile to develop it for the benefit of conventional fission fuel preparation and other various usages. Indian National Fusion Programme was initiated by indigenously developing the first Indian Tokamak, ADITYA, successfully commissioned in 1989 and has been generating interesting scientific results on various topics. The next major program at Institute for Plasma Research (IPR) has been to construct a Steady State Superconducting Tokamak (SST-1) by mix of import and indigenous development. After successful engineering validation of the subsystems in integrated operations, successful machine operation has been continued. Since then, the machine has been upgraded with a graphite first wall. As a strategy towards leapfrogging to save time, IPR and Department of Atomic Energy (DAE) decided on India’s participation in the International Thermonuclear Experimental Reactor (ITER) as a full partner, unique features of which will be its ability to operate for long durations and at power levels ∼500 MW sufficient to demonstrate the physics of burning plasma in a power plant like environment. It will also serve as a test-bed for additional fusion power plant technologies. To accelerate the domestic fusion research programme with integration of knowledge gained from ITER, we would embark upon design of a smaller fusion machine which will use already available technologies to produce controlled fusion reactions and use it as an energetic neutron source for test of materials developed for future fusion reactors

  3. Fusion Canada issue 13

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program. Included in this issue is a report on Canada's plans to participate in the Engineering Design Activities (EDA), bilateral meetings with Canada and the U.S., committee meeting with Canada-Europe, an update at Tokamak de Varennes on Plasma Biasing experiments and boronized graphite tests, fusion materials research at the University of Toronto using a dual beam accelerator and a review of the CFFTP and the CCFM. 2 figs

  4. Economically competitive fusion

    Directory of Open Access Journals (Sweden)

    David J. Ward

    2008-12-01

    Full Text Available Not since the oil crisis of the 1970s has the perception that energy is a crucial and precious resource been as strong as it is today. The need for a new approach to world energy supply, driven by concerns over resources, pollution, and security, is leading to a reappraisal of fusion. Fusion has enormous potential and major safety and environmental advantages, and hence could make a large difference to energy supplies.

  5. Cold nuclear fusion

    OpenAIRE

    Huang Zhenqiang Huang Yuxiang

    2013-01-01

    In normal temperature condition, the nuclear force constraint inertial guidance method, realize the combination of deuterium and tritium, helium and lithium... And with a magnetic moment of light nuclei controlled cold nuclear collide fusion, belongs to the nuclear energy research and development in the field of applied technology "cold nuclear collide fusion". According to the similarity of the nuclear force constraint inertial guidance system, the different velocity and energy of the ion be...

  6. Sonoluminescence and bubble fusion

    OpenAIRE

    Arakeri, Vijay H

    2003-01-01

    Sonoluminescence (SL), the phenomenon of light emission from nonlinear motion of a gas bubble, involves an extreme degree of energy focusing. The conditions within the bubble during the last stages of the nearly catastrophic implosion are thought to parallel the efforts aimed at developing inertial confinement fusion. A limited review on the topic of SL and its possible connection to bubble nuclear fusion is presented here. The emphasis is on looking for a link between the various forms o...

  7. Grassmannian Fusion Frames

    OpenAIRE

    King, Emily J.

    2010-01-01

    Transmitted data may be corrupted by both noise and data loss. Grassmannian frames are in some sense optimal representations of data transmitted over a noisy channel that may lose some of the transmitted coefficients. Fusion frame (or frame of subspaces) theory is a new area that has potential to be applied to problems in such fields as distributed sensing and parallel processing. Grassmannian fusion frames combine elements from both theories. A simple, novel construction of Grassmannian fusi...

  8. Fusion Simulation Program

    International Nuclear Information System (INIS)

    Many others in the fusion energy and advanced scientific computing communities participated in the development of this plan. The core planning team is grateful for their important contributions. This summary is meant as a quick overview the Fusion Simulation Program's (FSP's) purpose and intentions. There are several additional documents referenced within this one and all are supplemental or flow down from this Program Plan. The overall science goal of the DOE Office of Fusion Energy Sciences (FES) Fusion Simulation Program (FSP) is to develop predictive simulation capability for magnetically confined fusion plasmas at an unprecedented level of integration and fidelity. This will directly support and enable effective U.S. participation in International Thermonuclear Experimental Reactor (ITER) research and the overall mission of delivering practical fusion energy. The FSP will address a rich set of scientific issues together with experimental programs, producing validated integrated physics results. This is very well aligned with the mission of the ITER Organization to coordinate with its members the integrated modeling and control of fusion plasmas, including benchmarking and validation activities. (1). Initial FSP research will focus on two critical Integrated Science Application (ISA) areas: ISA1, the plasma edge; and ISA2, whole device modeling (WDM) including disruption avoidance. The first of these problems involves the narrow plasma boundary layer and its complex interactions with the plasma core and the surrounding material wall. The second requires development of a computationally tractable, but comprehensive model that describes all equilibrium and dynamic processes at a sufficient level of detail to provide useful prediction of the temporal evolution of fusion plasma experiments. The initial driver for the whole device model will be prediction and avoidance of discharge-terminating disruptions, especially at high performance, which are a critical

  9. Economically competitive fusion

    OpenAIRE

    Ward, David J.; Dudarev, Sergei L.

    2008-01-01

    Not since the oil crisis of the 1970s has the perception that energy is a crucial and precious resource been as strong as it is today. The need for a new approach to world energy supply, driven by concerns over resources, pollution, and security, is leading to a reappraisal of fusion. Fusion has enormous potential and major safety and environmental advantages, and hence could make a large difference to energy supplies.

  10. Conference on Norwegian fusion research

    International Nuclear Information System (INIS)

    The question of instituting a systematic research programme in Norway on aspects of thermonuclear and plasma physics has been raised. The conference here reported was intended to provide basic information on the status of fusion research internationally and to discuss a possible Norwegian programme. The main contributions covered the present status of fusion research, international cooperation, fusion research in small countries and minor laboratories, fusion research in Denmark and Sweden, and a proposed fusion experiment in Bergen. (JIW)

  11. Fusion, cold fusion, and space policy

    International Nuclear Information System (INIS)

    This paper critiques Americal science policy through a consideration of two examples-cold fusion and asteroid mining. It points out that the failure of central planning in science and technology policy is just as marked as in more mundane activities. It highlights the current low level of debate and points out some technical issues that need to be addressed. It concludes with evidence that the alliance of flawed policy options is further lowering the level of debate. (author)

  12. Perspectives of fusion power

    International Nuclear Information System (INIS)

    New and practically inexhaustible sources of energy must be developed for the period when oil, coal and uranium will become scarce and expensive. Nuclear fusion holds great promise as one of these practically inexhaustible energy sources. Based on the deuteriumtritium reaction with tritium obtained from naturally occuring lithium, which is also widely available in Europe, the accessible energy resources in the world are 3.1012 to 3.1016 toe; based on the deuterium-deuterium reaction, the deuterium content of the oceans corresponds to 1020 toe. It is presently envisaged that in order to establish fusion as a large-scale energy source, three major thresholds must be reached: - Scientific feasibility, - Technical feasibility, i.e. the proof that the basic technical problems of the fusion reactor can be solved. - Commercial feasibility, i.e. proof that fusion power reactors can be built on an industrial scale, can be operated reliably and produce usable energy at prices competitive with other energy sources. From the above it is clear that the route to commercial fusion will be long and costly and involve the solution of extremely difficult technical problems. In view of the many steps which have to be taken, it appears unlikely that commercial fusion power will be in general use within the next 50 years and by that time world-wide expenditure on research, development and demonstration may well have exceeded 100 Bio ECU. (author)

  13. Energy from inertial fusion

    International Nuclear Information System (INIS)

    This book contains 22 articles on inertial fusion energy (IFE) research and development written in the framework of an international collaboration of authors under the guidance of an advisory group on inertial fusion energy set up in 1991 to advise the IAEA. It describes the actual scientific, engineering and technological developments in the field of inertial confinement fusion (ICF). It also identifies ways in which international co-operation in ICF could be stimulated. The book is intended for a large audience and provides an introduction to inertial fusion energy and an overview of the various technologies needed for IFE power plants to be developed. It contains chapters on (i) the fundamentals of IFE; (ii) inertial confinement target physics; (iii) IFE power plant design principles (requirements for power plant drivers, solid state laser drivers, gas laser drivers, heavy ion drivers, and light ion drivers, target fabrication and positioning, reaction chamber systems, power generation and conditioning and radiation control, materials management and target materials recovery), (iv) special design issues (radiation damage in structural materials, induced radioactivity, laser driver- reaction chamber interfaces, ion beam driver-reaction chamber interfaces), (v) inertial fusion energy development strategy, (vi) safety and environmental impact, (vii) economics and other figures of merit; (viii) other uses of inertial fusion (both those involving and not involving implosions); and (ix) international activities. Refs, figs and tabs

  14. Fusion materials irradiations at MaRIE's fission fusion facility

    Energy Technology Data Exchange (ETDEWEB)

    Pitcher, Eric J [Los Alamos National Laboratory

    2010-10-06

    Los Alamos National Laboratory's proposed signature facility, MaRIE, will provide scientists and engineers with new capabilities for modeling, synthesizing, examining, and testing materials of the future that will enhance the USA's energy security and national security. In the area of fusion power, the development of new structural alloys with better tolerance to the harsh radiation environments expected in fusion reactors will lead to improved safety and lower operating costs. The Fission and Fusion Materials Facility (F{sup 3}), one of three pillars of the proposed MaRIE facility, will offer researchers unprecedented access to a neutron radiation environment so that the effects of radiation damage on materials can be measured in-situ, during irradiation. The calculated radiation damage conditions within the F{sup 3} match, in many respects, that of a fusion reactor first wall, making it well suited for testing fusion materials. Here we report in particular on two important characteristics of the radiation environment with relevancy to radiation damage: the primary knock-on atom spectrum and the impact of the pulse structure of the proton beam on temporal characteristics of the atomic displacement rate. With respect to both of these, analyses show the F{sup 3} has conditions that are consistent with those of a steady-state fusion reactor first wall.

  15. Fusion Energy Postdoctoral Research Program, Professional Development Program: FY 1987 annual report

    International Nuclear Information System (INIS)

    In FY 1986, Oak Ridge Associated Universities (ORAU) initiated two programs for the US Department of Energy (DOE), Office of Fusion Energy (OFE): the Fusion Energy Postdoctoral Research Program and the Fusion Energy Professional Development Program. These programs provide opportunities to conduct collaborative research in magnetic fusion energy research and development programs at DOE laboratories and contractor sites. Participants become trained in advanced fusion energy research, interact with outstanding professionals, and become familiar with energy-related national issues while making personal contributions to the search for solutions to scientific problems. Both programs enhance the national fusion energy research and development effort by providing channels for the exchange of scientists and engineers, the diffusion of ideas and knowledge, and the transfer of relevant technologies. These programs, along with the Magnetic Fusion Energy Science and Technology Fellowship Programs, compose the fusion energy manpower development programs administered by ORAU for DOE/OFE

  16. Osteoclast Fusion is Based on Heterogeneity Between Fusion Partners

    DEFF Research Database (Denmark)

    Hobolt-Pedersen, Anne-Sofie; Delaissé, Jean-Marie; Søe, Kent

    2014-01-01

    Bone-resorbing osteoclasts are formed through fusion of mononucleated precursors. Their choice of partners during the fusion process remains unclear. We hypothesized that osteoclasts are selective in their choice of fusion partner and that this selectivity is based on heterogeneity among the cells...... with respect to their maturation stage and their expression and cellular organization of fusion factors. Support for this hypothesis was found from immunofluorescence staining of the osteoclast fusion factors CD47, dendritic cell-specific transmembrane protein (DC-STAMP), and syncytin-1. These stainings...... fusion steps was also suggested from experiments with a CD47 blocking antibody, which resulted in an inhibition of the fusion of small osteoclasts. Conversely, blocking of connexin 43 affected the fusion of larger osteoclasts with four or more nuclei. The suggestion that different fusion factors function...

  17. Fusion program in Japan and prospect of inertial fusion

    International Nuclear Information System (INIS)

    Development of nuclear fusion as a practical energy source could provide great benefits for all mankind. This fact has been widely recognized and this task could be good example to pursue the goal with worldwide collaboration and friendship. Fusion energy development is one of the big scientific programs in Japan. Both Magnetic Fusion Energy (MFE) and Inertial Fusion Energy (IFE) are proceeded aiming to achieve clean, abundant and safe fusion energy. An overview of the fusion energy research and development in Japan is given

  18. Fusion Canada issue 29

    International Nuclear Information System (INIS)

    A short bulletin from the National Fusion Program highlighting in this issue Canada-Europe Accords: 5 year R and D collaboration for the International Thermonuclear Experimental Reactor (ITER) AECL is designated to arrange and implement the Memorandum of Understanding (MOU) and the ITER Engineering Design Activities (EDA) while EUROTAM is responsible for operating Europe's Fusion R and D programs plus MOU and EDA. The MOU includes tokamaks, plasma physics, fusion technology, fusion fuels and other approaches to fusion energy (as alternatives to tokamaks). STOR-M Tokamak was restarted at the University of Saskatchewan following upgrades to the plasma chamber to accommodate the Compact Toroid (CT) injector. The CT injector has a flexible attachment thus allowing for injection angle adjustments. Real-time video images of a single plasma discharge on TdeV showing that as the plasma density increases, in a linear ramp divertor, the plasma contact with the horizontal plate decreases while contact increases with the oblique plate. Damage-resistant diffractive optical elements (DOE) have been developed for Inertial Confinement Fusion (ICF) research by Gentac Inc. and the National Optics Institute, laser beam homogeniser and laser harmonic separator DOE can also be made using the same technology. Studies using TdeV indicate that a divertor will be able to pump helium from the tokamak with a detached-plasma divertor but helium extraction performance must first be improved, presently the deuterium:helium retention radio-indicates that in order to pump enough helium through a fusion reactor, too much deuterium-tritium fuel would be pumped out. 2 fig

  19. Advanced fusion reactor

    Energy Technology Data Exchange (ETDEWEB)

    Tomita, Yukihiro [National Inst. for Fusion Science, Toki, Gifu (Japan)

    2003-04-01

    The main subjects on fusion research are now on D-T fueled fusion, mainly due to its high fusion reaction rate. However, many issues are still remained on the wall loading by the 14 MeV neutrons. In the case of D-D fueled fusion, the neutron wall loading is still remained, though the technology related to tritium breeding is not needed. The p-{sup 6}Li and p-{sup 11}B fueled fusions are not estimated to be the next generation candidate until the innovated plasma confinement technologies come in useful to achieve the high performance plasma parameters. The fusion reactor of D-{sup 3}He fuels has merits on the smaller neutron wall loading and tritium handling. However, there are difficulties on achieving the high temperature plasma more than 100 keV. Furthermore the high beta plasma is needed to decrease synchrotron radiation loss. In addition, the efficiency of the direct energy conversion from protons coming out from fusion reaction is one of the key parameters in keeping overall power balance. Therefore, open magnetic filed lines should surround the plasma column. In this paper, we outlined the design of the commercial base reactor (ARTEMIS) of 1 GW electric output power configured by D-{sup 3}He fueled FRC (Field Reversed Configuration). The ARTEMIS needs 64 kg of {sup 3}He per a year. On the other hand, 1 million tons of {sup 3}He is estimated to be in the moon. The {sup 3}He of about 10{sup 23} kg are to exist in gaseous planets such as Jupiter and Saturn. (Y. Tanaka)

  20. Application of the JDL data fusion process model for cyber security

    Science.gov (United States)

    Giacobe, Nicklaus A.

    2010-04-01

    A number of cyber security technologies have proposed the use of data fusion to enhance the defensive capabilities of the network and aid in the development of situational awareness for the security analyst. While there have been advances in fusion technologies and the application of fusion in intrusion detection systems (IDSs), in particular, additional progress can be made by gaining a better understanding of a variety of data fusion processes and applying them to the cyber security application domain. This research explores the underlying processes identified in the Joint Directors of Laboratories (JDL) data fusion process model and further describes them in a cyber security context.

  1. Fusion of multiple sensor types in computer vision systems

    OpenAIRE

    Mayo, Donald R.

    2007-01-01

    This research provides analysis of several approaches to the fusion of multiple dissimilar sensors to supplement simple color vision detection and recognition. Non-visible sensor systems can enhance computer vision systems. Our research investigates using thermal infrared (IR) sensors in combination with color data for object detection and recognition. We analyze several types of high-level and low-level sensor fusion to compare error rates with raw color and raw IR error rates in detecti...

  2. Measurement of fusion cross section with neutron halo nuclei

    International Nuclear Information System (INIS)

    Fusion cross sections of 11Be, 10Be and 9Be have been measured on 209Bi target at 30-70MeV. Due to the neutron halo effect of 11Be, a large enhancement or suppression of the fusion cross section around the Coulomb barrier was theoretically predicted. Comparing the excitation function of 11Be with 10Be at near the Coulomb barrier region, no significant difference has been observed. ((orig.))

  3. Design of a Framework to Facilitate Decisions Using Information Fusion

    OpenAIRE

    Tamer M. Abo Neama; Ismail A. Ismail; Tarek S. Sobh; Zaki, M.

    2013-01-01

    Information fusion is an advanced research area which can assist decision makers in enhancing their decisions. This paper aims at designing a new multi-layer framework that can support the process of performing decisions from the obtained beliefs using information fusion. Since it is not an easy task to cross the gap between computed beliefs of certain hypothesis and decisions, the proposed framework consists of the following layers in order to provide a suitable architecture (ordered bottom ...

  4. Near-barrier Fusion of 32S+94Zr

    Institute of Scientific and Technical Information of China (English)

    JIA; Hui-ming; LIN; Cheng-jian; ZHANG; Huan-qiao; LIU; Zu-hua; YANG; Feng; XU; Xin-xing; YANG; Lei; BAO; Peng-fei; SUN; Li-jie; MA; Nan-ru

    2013-01-01

    Coupled-channels(CC)effect is a general phenomenon in nature and plays a crucial role in near-barrier heavy-ion nuclear reactions.The first CC effect,due to static deformation,was discovered in16O+ASm by Stokstad et al.by a direct comparison of the experimental fusion excitation functions.Later,a correlation between the overall transfer strength and the fusion enhancement was noticed by Heinning et

  5. Lateral Lumbar Interbody Fusion.

    Science.gov (United States)

    Pawar, Abhijit; Hughes, Alexander; Girardi, Federico; Sama, Andrew; Lebl, Darren; Cammisa, Frank

    2015-12-01

    The lateral lumbar interbody fusion (LLIF) is a relatively new technique that allows the surgeon to access the intervertebral space from a direct lateral approach either anterior to or through the psoas muscle. This approach provides an alternative to anterior lumbar interbody fusion with instrumentation, posterior lumbar interbody fusion, and transforaminal lumbar interbody fusion for anterior column support. LLIF is minimally invasive, safe, better structural support from the apophyseal ring, potential for coronal plane deformity correction, and indirect decompression, which have has made this technique popular. LLIF is currently being utilized for a variety of pathologies including but not limited to adult de novo lumbar scoliosis, central and foraminal stenosis, spondylolisthesis, and adjacent segment degeneration. Although early clinical outcomes have been good, the potential for significant neurological and vascular vertebral endplate complications exists. Nevertheless, LLIF is a promising technique with the potential to more effectively treat complex adult de novo scoliosis and achieve predictable fusion while avoiding the complications of traditional anterior surgery and posterior interbody techniques. PMID:26713134

  6. Fusion technology programme

    International Nuclear Information System (INIS)

    In 1982, KfK joined the fusion programme of EURATOM as a further association introducing its experience in nuclear technology. KfK closely cooperates with IPP Garching, the two institutions forming a research unit aiming at planning and realization of future development steps of fusion. KfK has combined its forces in the Nuclear Fusion Project (PKF) with participation of several KfK departments to the project tasks. Previous work of KfK in magnetic fusion has addressed mainly superconducting magnets, plasma heating by cluster ions and studies on structural materials. At present, emphasis of our work has concentrated increasingly on the nuclear part, i.e. the first wall and blanket structures and the elements of the tritium extraction and purification system. Associated to this component development are studies of remote maintenance and safety. Most of the actual work addresses NET, the next step to a demonstration of fusion feasibility. NET is supposed to follow JET, the operating plasma physics experiment of Euratom, on the 1990's. Detailed progress of the work in the past half year is described in this report. (orig./GG)

  7. HIV-1 virion fusion assay: uncoating not required and no effect of Nef on fusion

    International Nuclear Information System (INIS)

    We recently described a sensitive and specific assay that detects the fusion of HIV-1 virions to a broad range of target cells, including primary CD4 cells. This assay involves the use of virions containing β-lactamase-Vpr (BlaM-Vpr) and the loading of target cells with CCF2, a fluorogenic substrate of β-lactamase. Since Vpr strongly associates with the viral core, uncoating of the viral particle might be required for effective cleavage of CCF2 by BlaM-Vpr. Here, we show that BlaM-Vpr within mature viral cores effectively cleaves CCF2, indicating that this assay measures virion fusion independently of uncoating. We also show that wildtype and Nef-deficient HIV-1 virions fuse with equivalent efficiency to HeLa-CD4 cells, SupT1 T cells, and primary CD4 T cells. Since Nef enhances cytoplasmic delivery of viral cores and increases viral infectivity, these findings indicate that Nef enhances an early post-fusion event in the multistep process of viral entry. Possible sites of Nef action include enlargement of the fusion pore, enhanced uncoating of viral particles, and more efficient passage of viral cores through the dense cortical actin network located immediately beneath the plasma membrane

  8. Coatings for fusion reactor environments

    International Nuclear Information System (INIS)

    The internal surfaces of a tokamak fusion reactor control the impurity injection and gas recycling into the fusion plasma. Coating of internal surfaces may provide a desirable and possibly necessary design flexibility for achieving the temperatures, ion densities and containment times necessary for net energy production from fusion reactions to take place. In this paper the reactor environments seen by various componentare reviewed along with possible materials responses. Characteristics of coating-substrate systems, important to fusion applications, are delineated and the present status of coating development for fusion applications is reviewed. Coating development for fusion applications is just beginning and poses a unique and important challenge for materials development

  9. Fusion: Energy for the future

    International Nuclear Information System (INIS)

    Fusion, which occurs in the sun and the stars, is a process of transforming matter into energy. If we can harness the fusion process on Earth, it opens the way to assuring that future generations will not want for heat and electric power. The purpose of this booklet is to introduce the concept of fusion energy as a viable, environmentally sustainable energy source for the twenty-first century. The booklet presents the basic principles of fusion, the global research and development effort in fusion, and Canada's programs for fusion research and development

  10. Physics of magnetic confinement fusion

    OpenAIRE

    Wagner F

    2013-01-01

    Fusion is the energy source of the universe. The local conditions in the core of the Sun allow the transfer of mass into energy, which is finally released in the form of radiation. Technical fusion melts deuterons and tritons to helium releasing large amounts of energy per fusion process. Because of the conditions for fusion, which will be deduced, the fusion fuel is in the plasma state. Here we report on the confinement of fusion plasmas by magnetic fields. Different confinement concepts — t...

  11. High Levels of Exosomes Expressing CD63 and Caveolin-1 in Plasma of Melanoma Patients

    OpenAIRE

    Logozzi, Mariantonia; De Milito, Angelo; Lugini, Luana; Borghi, Martina; Calabrò, Luana; Spada, Massimo; Perdicchio, Maurizio; MARINO, MARIA LUCIA; Federici, Cristina; Iessi, Elisabetta; Brambilla, Daria; Venturi, Giulietta; Lozupone, Francesco; Santinami, Mario; Huber, Veronica

    2009-01-01

    Background Metastatic melanoma is an untreatable cancer lacking reliable and non-invasive markers of disease progression. Exosomes are small vesicles secreted by normal as well as tumor cells. Human tumor-derived exosomes are involved in malignant progression and we evaluated the presence of exosomes in plasma of melanoma patients as a potential tool for cancer screening and follow-up. Methodology/Principal Findings We designed an in-house sandwich ELISA (Exotest) to capture and quantify exos...

  12. Temporal evolution in caveolin 1 methylation levels during human esophageal carcinogenesis

    International Nuclear Information System (INIS)

    Esophageal cancer ranks eighth among frequent cancers worldwide. Our aim was to investigate whether and at which neoplastic stage promoter hypermethylation of CAV1 is involved in human esophageal carcinogenesis. Using real-time quantitative methylation-specific PCR (qMSP), we examined CAV1 promoter hypermethylation in 260 human esophageal tissue specimens. Real-time RT-PCR and qMSP were also performed on OE33 esophageal cancer cells before and after treatment with the demethylating agent, 5-aza-2’-deoxycytidine (5-Aza-dC). CAV1 hypermethylation showed highly discriminative ROC curve profiles, clearly distinguishing esophageal adenocarcinomas (EAC) and esophageal squamous cell carcinomas (ESCC) from normal esophagus (NE) (EAC vs. NE, AUROC = 0.839 and p < 0.0001; ESCC vs. NE, AUROC = 0.920 and p < 0.0001). Both CAV1 methylation frequency and normalized methylation value (NMV) were significantly higher in Barrett’s metaplasia (BE), low-grade and high-grade dysplasia occurring in BE (D), EAC, and ESCC than in NE (all p < 0.01, respectively). Meanwhile, among 41 cases with matched NE and EAC or ESCC, CAV1 NMVs in EAC and ESCC (mean = 0.273) were significantly higher than in corresponding NE (mean = 0.146; p < 0.01, Student’s paired t-test). Treatment of OE33 EAC cells with 5-Aza-dC reduced CAV1 methylation and increased CAV1 mRNA expression. CAV1 promoter hypermethylation is a frequent event in human esophageal carcinomas and is associated with early neoplastic progression in Barrett’s esophagus

  13. Fibronectin Matrix Turnover Occurs through a Caveolin-1–dependent Process

    OpenAIRE

    Sottile, Jane; Chandler, Jennifer

    2005-01-01

    Extracellular matrix remodeling occurs during development, tissue repair, and in a number of pathologies, including fibrotic disorders, hypertension, and atherosclerosis. Extracellular matrix remodeling involves the complex interplay between extracellular matrix synthesis, deposition, and degradation. Factors that control these processes are likely to play key roles in regulating physiological and pathological extracellular matrix remodeling. Our data show that fibronectin polymerization into...

  14. The Role of Fatty Acids and Caveolin-1 in TNF-α-Induced Endothelial Cell Activation

    OpenAIRE

    Wang, Lei; Lim, Eun-Jin; Toborek, Michal; Hennig, Bernhard

    2008-01-01

    Hypertriglyceridemia and associated high circulating free fatty acids are important risk factors of atherosclerosis. In contrast to omega-3 fatty acids, linoleic acid, the major omega-6 unsaturated fatty acid in the American diet, may be atherogenic by amplifying an endothelial inflammatory response. We hypothesize that omega-6 and omega-3 fatty acids can differentially modulate TNF-α-induced endothelial cell activation and that functional plasma membrane microdomains called caveolae are requ...

  15. Canadian fusion program

    International Nuclear Information System (INIS)

    The National Research Council of Canada is establishing a coordinated national program of fusion research and development that is planned to grow to a total annual operating level of about $20 million in 1985. The long-term objective of the program is to put Canadian industry in a position to manufacture sub-systems and components of fusion power reactors. In the near term the program is designed to establish a minimum base of scientific and technical expertise sufficient to make recognized contributions and thereby gain access to the international effort. The Canadian program must be narrowly focussed on a few specializations where Canada has special indigenous skills or technologies. The programs being funded are the Tokamak de Varennes, the Fusion Fuels Technology Project centered on tritium management, and high-power gas laser technology and associated diagnostic instrumentation

  16. Inverse fusion PCR cloning.

    Directory of Open Access Journals (Sweden)

    Markus Spiliotis

    Full Text Available Inverse fusion PCR cloning (IFPC is an easy, PCR based three-step cloning method that allows the seamless and directional insertion of PCR products into virtually all plasmids, this with a free choice of the insertion site. The PCR-derived inserts contain a vector-complementary 5'-end that allows a fusion with the vector by an overlap extension PCR, and the resulting amplified insert-vector fusions are then circularized by ligation prior transformation. A minimal amount of starting material is needed and experimental steps are reduced. Untreated circular plasmid, or alternatively bacteria containing the plasmid, can be used as templates for the insertion, and clean-up of the insert fragment is not urgently required. The whole cloning procedure can be performed within a minimal hands-on time and results in the generation of hundreds to ten-thousands of positive colonies, with a minimal background.

  17. Fusion research at ORNL

    International Nuclear Information System (INIS)

    The ORNL Fusion Program includes the experimental and theoretical study of two different classes of magnetic confinement schemes - systems with helical magnetic fields, such as the tokamak and stellarator, and the ELMO Bumpy Torus (EBT) class of toroidally linked mirror systems; the development of technologies, including superconducting magnets, neutral atomic beam and radio frequency (rf) heating systems, fueling systems, materials, and diagnostics; the development of databases for atomic physics and radiation effects; the assessment of the environmental impact of magnetic fusion; and the design of advanced demonstration fusion devices. The program involves wide collaboration, both within ORNL and with other institutions. The elements of this program are shown. This document illustrates the program's scope; and aims by reviewing recent progress

  18. Colliding Beam Fusion Reactors

    Science.gov (United States)

    Rostoker, Norman; Qerushi, Artan; Binderbauer, Michl

    2003-06-01

    The recirculating power for virtually all types of fusion reactors has previously been calculated [1] with the Fokker-Planck equation. The reactors involve non-Maxwellian plasmas. The calculations are generic in that they do not relate to specific confinement devices. In all cases except for a Tokamak with D-T fuel the recirculating power was found to exceed the fusion power by a large factor. In this paper we criticize the generality claimed for this calculation. The ratio of circulating power to fusion power is calculated for the Colliding Beam Reactor with fuels D-T, D-He3 and p-B11. The results are respectively, 0.070, 0.141 and 0.493.

  19. Fusion Reactor Materials

    Energy Technology Data Exchange (ETDEWEB)

    Decreton, M

    2002-04-01

    The objective of SCK-CEN's programme on fusion reactor materials is to contribute to the knowledge on the radiation-induced behaviour of fusion reactor materials and components as well as to help the international community in building the scientific and technical basis needed for the construction of the future reactor. Ongoing projects include: the study of the mechanical and chemical (corrosion) behaviour of structural materials under neutron irradiation and water coolant environment; the investigation of the characteristics of irradiated first wall material such as beryllium; investigations on the management of materials resulting from the dismantling of fusion reactors including waste disposal. Progress and achievements in these areas in 2001 are discussed.

  20. Ceramics for fusion devices

    International Nuclear Information System (INIS)

    Ceramics are required for a number of applications in fusion devices, among the most critical of which are magnetic coil insulators, windows for RF heating systems, and structural uses. Radiation effects dominate consideration of candidate materials, although good pre-irradiation properties are a requisite. Materials and components can be optimized by careful control of chemical and microstructural content, and application of brittle material design and testing techniques. Future directions for research and development should include further extension of the data base in the areas of electrical, structural, and thermal properties; establishment of a fission neutron/fusion neutron correlation including transmutation gas effects; and development of new materials tailored to meet the specific needs of fusion reactors

  1. Sensor Data Fusion

    DEFF Research Database (Denmark)

    Plascencia, Alfredo; Stepán, Petr

    2006-01-01

    The main contribution of this paper is to present a sensor fusion approach to scene environment mapping as part of a Sensor Data Fusion (SDF) architecture. This approach involves combined sonar array with stereo vision readings.  Sonar readings are interpreted using probability density functions...... to the occupied and empty regions. Scale Invariant Feature Transform (SIFT) feature descriptors are interpreted using gaussian probabilistic error models. The use of occupancy grids is proposed for representing the sensor readings. The Bayesian estimation approach is applied to update the sonar array......  and the SIFT descriptors' uncertainty grids. The sensor fusion yields a significant reduction in the uncertainty of the occupancy grid compared to the individual sensor readings....

  2. Alternate laser fusion drivers

    International Nuclear Information System (INIS)

    One objective of research on inertial confinement fusion is the development of a power generating system based on this concept. Realization of this goal will depend on the availability of a suitable laser or other system to drive the power plant. The primary laser systems used for laser fusion research, Nd3+: Glass and CO2, have characteristics which may preclude their use for this application. Glass lasers are presently perceived to be incapable of sufficiently high average power operation and the CO2 laser may be limited by and issues associated with target coupling. These general perceptions have encouraged a search for alternatives to the present systems. The search for new lasers has been directed generally towards shorter wavelengths; most of the new lasers discovered in the past few years have been in the visible and ultraviolet region of the spectrum. Virtually all of them have been advocated as the most promising candidate for a fusion driver at one time or another

  3. Laser inertial fusion

    International Nuclear Information System (INIS)

    Although the energy of the radiation pulses generated by the world's largest laser facilities approach the MJ limit, it is still lower than that calculated on means of the Lawson criterion for thermonuclear fusion. The severe energy requirements can be weakened by efficient pre-compression of laser targets and by non-linear conversion of laser radiation to shorter wavelengths. The homogeneity of the laser target irradiation can be improved by using multi-beam or indirect-drive laser schemes. The neodymium laser facilities such as Nova Upgrade in Livermore or Gekko XII in Osaca are still the most promising fusion drivers, but the chances of powerful iodine lasers such as ISKRA 5 in Arzamas and Asterix in Munich are also high. The prospects of these and other laser fusion drivers are critically assessed and the role of smaller devices as the Prague iodine laser Perun is discussed. (J.U.) 6 figs., 7 refs

  4. Spherical torus fusion reactor

    Science.gov (United States)

    Peng, Yueng-Kay M.

    1989-01-01

    A fusion reactor is provided having a near spherical-shaped plasma with a modest central opening through which straight segments of toroidal field coils extend that carry electrical current for generating a toroidal magnet plasma confinement fields. By retaining only the indispensable components inboard of the plasma torus, principally the cooled toroidal field conductors and in some cases a vacuum containment vessel wall, the fusion reactor features an exceptionally small aspect ratio (typically about 1.5), a naturally elongated plasma cross section without extensive field shaping, requires low strength magnetic containment fields, small size and high beta. These features combine to produce a spherical torus plasma in a unique physics regime which permits compact fusion at low field and modest cost.

  5. Fusion technology development plan

    International Nuclear Information System (INIS)

    This Fusion Technology Development Plan (FTDP) has been prepared to show how the technology development program conducted by the Division of Development and Technology of the Office of Fusion Energy supports the overall magnetic fusion energy program as delineated in the March 17, 1983, DOE testimony before the Energy Research and Production Subcommittee of the House Committee on Science and Technology. A first draft of this plan distributed for comment in November 1981. since that draft was prepared, changes in expectations for funding in the program have led us to develop a set of priorities based on critical technology issues. These critical issues and the priority ranking of technology development efforts was accomplished with help from each of the major program participants

  6. Mirror Fusion Test Facility

    International Nuclear Information System (INIS)

    On October 1, 1977 work began at LLL on the Mirror Fusion Test Facility (MFTF), an advanced experimental fusion device. Scheduled for operation in late 1981, MFTF is designed as an intermediate step between present mirror machines, such as 2XIIB, and an experimental fusion reactor. This design incorporates improved technology and a better theoretical understanding of how neutral beam injection, plasma guns, and gas injection into the plasma region compensate for cooling and particle losses. With the new facility, we expect to achieve a confinement factor (n tau) of 1012 particles . sm/cm3--a tenfold increase over 2XIIB n tau values--and to increase plasma temperature to over 500 million K. The following article describes this new facility and reports on progress in some of the R and D projects that are providing the technological base for its construction

  7. Cold fusion: an outlaw

    International Nuclear Information System (INIS)

    This article presents a status report on cold fusion. The experiment conducted by S.Pons and M.Fleischmann suggests that nuclear reactions can be triggered by particular chemical conditions. This experiment was difficult to reproduce because of the great number of variables that are to be mastered. The Stanford Research Institute realised an extensive study of this experiment. Its results show that an energy excess occurs in certain conditions and is very dependent on the quality of involved materials: some batches of palladium are more active than others. The author draws a parallel between cold fusion and the latest works of Japanese and Russian scientists which show that nuclear reactions can be induced by biological processes. A review of theoretical models explaining the fusion process is made and an extensive bibliography is given. (A.C.)

  8. Medical Image Fusion

    Directory of Open Access Journals (Sweden)

    Mitra Rafizadeh

    2007-08-01

    Full Text Available Technological advances in medical imaging in the past two decades have enable radiologists to create images of the human body with unprecedented resolution. MRI, PET,... imaging devices can quickly acquire 3D images. Image fusion establishes an anatomical correlation between corresponding images derived from different examination. This fusion is applied either to combine images of different modalities (CT, MRI or single modality (PET-PET."nImage fusion is performed in two steps:"n1 Registration: spatial modification (eg. translation of model image relative to reference image in order to arrive at an ideal matching of both images. Registration methods are feature-based and intensity-based approaches."n2 Visualization: the goal of it is to depict the spatial relationship between the model image and refer-ence image. We can point out its clinical application in nuclear medicine (PET/CT.

  9. Insulators for fusion applications

    International Nuclear Information System (INIS)

    Design studies for fusion devices and reactors have become more detailed in recent years and with this has come a better understanding of requirements and operating conditions for insulators in these machines. Ceramic and organic insulators are widely used for many components of fusion devices and reactors namely: radio frequency (RF) energy injection systems (BeO, Al2O3, Mg Al2O4, Si3N4); electrical insulation for the torus structure (SiC, Al2O3, MgO, Mg Al2O4, Si4Al2O2N6, Si3N4, Y2O3); lightly-shielded magnetic coils (MgO, MgAl2O4); the toroidal field coil (epoxies, polyimides), neutron shield (B4C, TiH2); high efficiency electrical generation; as well as the generation of very high temperatures for high efficiency hydrogen production processes (ZrO2 and Al2O3 - mat, graphite and carbon - felt). Timely development of insulators for fusion applications is clearly necessary. Those materials to be used in fusion machines should show high resistance to radiation damage and maintain their structural integrity. Now the need is urgent for a variety of radiation resistant materials, but much effort in these areas is required for insulators to be considered seriously by the design community. This document contains 14 papers from an IAEA meeting. It was the objective of this meeting to identify existing problems in analysing various situations of applications and requirements of electrical insulators and ceramics in fusion and to recommend strategies and different stages of implementation. This meeting was endorsed by the International Fusion Research Council

  10. Neutrons and fusion

    International Nuclear Information System (INIS)

    The production of energy from fusion reactions does not require neutrons in the fundamental sense that they are required in a fission reactor. Nevertheless, the dominant fusion reaction, that between deuterium and tritium, yields a 14 MeV neutron. To contrast a fusion reactor based on this reaction with the fission case, 3 x 1020 such neutrons produced per gigawatt of power. This is four times as many neutrons as in an equivalent fission reactor and they carry seven times the energy of the fission neutrons. Thus, they dominate the energy recovery problem and create technological problems comparable to the original plasma confinement problem as far as a practical power producing device is concerned. Further contrasts of the fusion and fission cases are presented to establish the general role of neutrons in fusion devices. Details of the energy deposition processes are discussed and those reactions necessary for producing additional tritium are outlined. The relatively high energy flux with its large intensity will activate almost any materials of which the reactor may be composed. This activation is examined from the point of view of decay heat, radiological safety, and long-term storage. In addition, a discussion of the deleterious effects of neutron interactions on materials is given in some detail; this includes the helium and hydrogen producing reactions and displacement rate of the lattice atoms. The various materials that have been proposed for structural purposes, for breeding, reflecting, and moderating neutrons, and for radiation shielding are reviewed from the nuclear standpoint. The specific reactions of interest are taken up for various materials and finally a report is given on the status and prospects of data for fusion studies

  11. Implication des peptides de fusion des glycoprotéines de fusion virales de classe I dans la fusion membranaire

    OpenAIRE

    Brasseur R.; Charloteaux B.; Lins L.; Lorin A.

    2007-01-01

    The implication of fusion peptides of class I viral fusion glycoproteins in the membrane fusion. Viral infection involves fusion between the viral envelope and the target cell plasmic membrane. The fusion is induced by a glycoprotein anchored in the viral envelope. After activation, the glycoprotein undergoes a conformational change inducing the exposure of a region named « fusion peptide » essential for the fusion process. Studies on glycoproteins and on isolated fusion peptides have allowed...

  12. Atomic data for fusion

    International Nuclear Information System (INIS)

    This report provides a handbook of recommended cross-section and rate-coefficient data for inelastic collisions between hydrogen, helium and lithium atoms, molecules and ions, and encompasses more than 400 different reactions of primary interest in fusion research. Published experimental and theoretical data have been collected and evaluated, and the recommended data are presented in tabular, graphical and parametrized form. Processes include excitation and spectral line emission, charge exchange, ionization, stripping, dissociation and particle interchange reactions. The range of collision energies is appropriate to applications in fusion-energy research

  13. Alternate fusion concepts

    International Nuclear Information System (INIS)

    This review summarizes the status of alternate fusion concepts and plans for their future. The concepts selected for review are those employing electromagnetic confinement for which there have been reasonable predictions of net energy gain from pure fusion and which have shown significant recent development or are the subjects of ongoing international activity. They include advanced tokamaks, stellarators, the spherical torus, reversed-field pinch and dense z-field pinch devices, field reversed configuration, and spheromaks. In addition, an overall view of the status of each concept with respect to achieving ignition and to reactor designs is presented

  14. Fusiones transfronterizas intracomunitarias

    OpenAIRE

    Garcia Wintzer, Laura

    2015-01-01

    A lo largo de este trabajo llevará a cabo un estudio sobre las fusiones transfronterizas intracomunitarias. Dado que es una figura relativamente nueva en el ordenamiento jurídico, tanto a nivel español como europeo, se ha considerado oportuno estudiar dicha operación desde diferentes perspectivas. La elección de las fusiones transfronterizas intracomunitarias como objeto de es-tudio viene a partir de la valoración de las diferentes modificaciones estructurales existentes en el ámbito mercanti...

  15. Cold fusion in perspective

    International Nuclear Information System (INIS)

    Since early April a great deal of excitement has been created over the Fleischmann/Pons cold fusion experiment, which if it performs as advertised, could turn out to be mankind's best hope of heading off the energy crisis scheduled for early in the next century. Dozens of groups around the world are now attempting to duplicate the experiment to see if Fleischmann and Pons' discovery is an experimental mistake, an unknown electrochemical effect or a new kind of fusion reaction. This article puts the experiment into the perspective of today and looks at how it might affect the energy scene tomorrow if it should turn out to be commercially exploitable. (author)

  16. Advanced fusion concepts program

    International Nuclear Information System (INIS)

    While the prospects for the eventual development of a tokamak-based fusion reactor appear promising at the present time, the Department of Energy maintains a vigorous program in alternate magnetic fusion concepts. Several of the concepts presently supported include the toroidal reversed field pinch, Tormac, Elmo Bumpy Torus, and various linear options. Recent technical accomplishments and program evaluations indicate that the possibility now exists for undertaking the next development stage, a proof-of-principle experiment, for a few of the most promising alternate concepts

  17. Fusion technology programme

    International Nuclear Information System (INIS)

    In the current Fusion Technology Programme of the European Community the KfK association is working at present on 16 R and D contracts. Most of the work is strongly oriented towards the Next European Torus. Direct support to NET is given by three KfK delegates being member of the NET study group. In addition to the R and D contracts the association is working on 11 NET study contracts. Though KfK contributes to all areas defined in fusion technology, the main emphasis is put on superconducting magnet and breeding blanket development. Other important fields are tritium technology, materials research, and remote handling. (orig./GG)

  18. The European Fusion Programme

    International Nuclear Information System (INIS)

    The European Fusion Programme is coordinated by Euratom and represents a long term cooperative project of Member States of the European Communities in the field of fusion, designed to lead to the joint construction of prototypes. The main lines of the programme proposed for 1982 to 1986 are: (1) the continuation of a strong effort on tokamaks with emphasis on JET construction, operation and upgrading, (2) conceptual design of NET and development of the related technology, and (3) further work on two alternative magnetic confinement systems. The current status and future plans for this programme are discussed in the paper. (author)

  19. Fusion's $372-Million Mothball

    International Nuclear Information System (INIS)

    Lawrence Livermore National Laboratory's Mirror Fusion Test Facility (MFTF-B) was built to prove that a design using magnetic mirrors could compete in an international race to produce a commercial fusion reactor. The MFTF-B was to be used to propel the magnetic mirror program into a real contest against Livermore's arch rival: the Princeton Plasma Physics Laboratory and its tokamak machine. On 21 February 1986, after 9 years of construction and $372 million, MTFT-B was officially dedicated. However, due to budget restriction DOE demanded the facility be put on standby the very next day

  20. Atomic data for fusion

    Energy Technology Data Exchange (ETDEWEB)

    Hunter, H.T.; Kirkpatrick, M.I.; Alvarez, I.; Cisneros, C.; Phaneuf, R.A. (eds.); Barnett, C.F.

    1990-07-01

    This report provides a handbook of recommended cross-section and rate-coefficient data for inelastic collisions between hydrogen, helium and lithium atoms, molecules and ions, and encompasses more than 400 different reactions of primary interest in fusion research. Published experimental and theoretical data have been collected and evaluated, and the recommended data are presented in tabular, graphical and parametrized form. Processes include excitation and spectral line emission, charge exchange, ionization, stripping, dissociation and particle interchange reactions. The range of collision energies is appropriate to applications in fusion-energy research.