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Sample records for cave molly poecilia

  1. Sex-specific local life-history adaptation in surface- and cave-dwelling Atlantic mollies (Poecilia mexicana).

    Science.gov (United States)

    Riesch, Rüdiger; Reznick, David N; Plath, Martin; Schlupp, Ingo

    2016-03-10

    Cavefishes have long been used as model organisms showcasing adaptive diversification, but does adaptation to caves also facilitate the evolution of reproductive isolation from surface ancestors? We raised offspring of wild-caught surface- and cave-dwelling ecotypes of the neotropical fish Poecilia mexicana to sexual maturity in a 12-month common garden experiment. Fish were raised under one of two food regimes (high vs. low), and this was crossed with differences in lighting conditions (permanent darkness vs. 12:12 h light:dark cycle) in a 2 × 2 factorial design, allowing us to elucidate potential patterns of local adaptation in life histories. Our results reveal a pattern of sex-specific local life-history adaptation: Surface molly females had the highest fitness in the treatment best resembling their habitat of origin (high food and a light:dark cycle), and suffered from almost complete reproductive failure in darkness, while cave molly females were not similarly affected in any treatment. Males of both ecotypes, on the other hand, showed only weak evidence for local adaptation. Nonetheless, local life-history adaptation in females likely contributes to ecological diversification in this system and other cave animals, further supporting the role of local adaptation due to strong divergent selection as a major force in ecological speciation.

  2. Otolith morphology and hearing abilities in cave- and surface-dwelling ecotypes of the Atlantic molly, Poecilia mexicana (Teleostei: Poeciliidae)

    Science.gov (United States)

    Schulz-Mirbach, Tanja; Ladich, Friedrich; Riesch, Rüdiger; Plath, Martin

    2010-01-01

    Cave fish have rarely been investigated with regard to their inner ear morphology, hearing abilities, and acoustic communication. Based on a previous study that revealed morphological differences in the saccular otolith between a cave and two surface populations of Poecilia mexicana, we checked for additional differences in utricular and lagenar otoliths and tested whether different populations have similar hearing sensitivities. We found pronounced differences in the shape of all three otoliths. Otoliths of the saccule and lagena from cave fish differed from those of surface fish in the features of the face oriented towards the sensory epithelium. In addition, otoliths of the utricle and lagena were significantly heavier in cave fish. Auditory sensitivities were measured between 100 and 1500 Hz, utilizing the auditory evoked potential recording technique. We found similar hearing abilities in cave and surface fish, with greatest sensitivity between 200 and 300 Hz. An acoustic survey revealed that neither ecotype produced species-specific sounds. Our data indicate that cave dwelling altered the otolith morphology in Atlantic mollies, probably due to metabolic differences. Different otolith morphology, however, did not affect general auditory sensitivity or acoustic behavior. PMID:20430090

  3. Microsatellites for the gynogenetic Amazon molly, Poecilia formosa

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 85; Issue 1. Microsatellites for the gynogenetic Amazon molly, Poecilia formosa: useful tools for detection of mutation rate, ploidy determination and overall genetic diversity. Kathrin P. Lampert Dunja K. Lamatsch Susanne Schories Armin Hopf Francisco J. García De León ...

  4. Insights into the Social Behavior of Surface and Cave-Dwelling Fish (Poecilia mexicana in Light and Darkness through the Use of a Biomimetic Robot

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    David Bierbach

    2018-02-01

    Full Text Available Biomimetic robots (BRs are becoming more common in behavioral research and, if they are accepted as conspecifics, allow for new forms of experimental manipulations of social interactions. Nevertheless, it is often not clear which cues emanating from a BR are actually used as communicative signals and how species or populations with different sensory makeups react to specific types of BRs. We herein present results from experiments using two populations of livebearing fishes that differ in their sensory capabilities. In the South of Mexico, surface-dwelling mollies (Poecilia mexicana successfully invaded caves and adapted to dark conditions. While almost without pigment, these cave mollies possess smaller but still functional eyes. Although previous studies found cave mollies to show reduced shoaling preferences with conspecifics in light compared to surface mollies, it is assumed that they possess specialized adaptations to maintain some kind of sociality also in their dark habitats. By testing surface- and cave-dwelling mollies with RoboFish, a BR made for use in laboratory experiments with guppies and sticklebacks, we asked to what extent visual and non-visual cues play a role in their social behavior. Both cave- and surface-dwelling mollies followed the BR as well as a live companion when tested in light. However, when tested in darkness, only surface-dwelling fish were attracted by a live conspecific, whereas cave-dwelling fish were not. Neither cave- nor surface-dwelling mollies were attracted to RoboFish in darkness. This is the first study to use BRs for the investigation of social behavior in mollies and to compare responses to BRs both in light and darkness. As our RoboFish is accepted as conspecific by both used populations of the Atlantic molly only under light conditions but not in darkness, we argue that our replica is providing mostly visual cues.

  5. Microhabitat use, population densities, and size distributions of sulfur cave-dwelling Poecilia mexicana.

    Science.gov (United States)

    Jourdan, Jonas; Bierbach, David; Riesch, Rüdiger; Schießl, Angela; Wigh, Adriana; Arias-Rodriguez, Lenin; Indy, Jeane Rimber; Klaus, Sebastian; Zimmer, Claudia; Plath, Martin

    2014-01-01

    The Cueva del Azufre in Tabasco, Mexico, is a nutrient-rich cave and its inhabitants need to cope with high levels of dissolved hydrogen sulfide and extreme hypoxia. One of the successful colonizers of this cave is the poeciliid fish Poecilia mexicana, which has received considerable attention as a model organism to examine evolutionary adaptations to extreme environmental conditions. Nonetheless, basic ecological data on the endemic cave molly population are still missing; here we aim to provide data on population densities, size class compositions and use of different microhabitats. We found high overall densities in the cave and highest densities at the middle part of the cave with more than 200 individuals per square meter. These sites have lower H2S concentrations compared to the inner parts where most large sulfide sources are located, but they are annually exposed to a religious harvesting ceremony of local Zoque people called La Pesca. We found a marked shift in size/age compositions towards an overabundance of smaller, juvenile fish at those sites. We discuss these findings in relation to several environmental gradients within the cave (i.e., differences in toxicity and lighting conditions), but we also tentatively argue that the annual fish harvest during a religious ceremony (La Pesca) locally diminishes competition (and possibly, cannibalism by large adults), which is followed by a phase of overcompensation of fish densities.

  6. Hepatic lesions in mollies (Poecilia latipinna) collected from Bayou Trepagnier, Louisiana

    International Nuclear Information System (INIS)

    Thiyagarajah, A.

    1993-01-01

    Mollies, Poecilia latipinna, are small fish species belonging to the Family Poeciliidae. Mollies are surface feeders and are commonly found in Louisiana waters. Bayou Trepagnier is located in the Lake Pontchatrain Basin, in St. Charles Parish of Louisiana, which receives treated wastewater and stormwater from an oil refinery and manufacturing complex. The purpose of this study was to determine the effects of refinery discharges on mollies from Bayou Trepagnier. Fish were caught by beach seine, examined for gross lesions and then fixed in 10% neutral buffered formalin for histopathological analysis. Paraffin-embedded fish were cut at 6 μm and stained with hematoxylin and eosin. Lesions observed in mollies were grouped into (1) neoplasms, (2) preneoplastic lesions, and (3) cytotoxic lesions. Hepatocellular carcinoma was the only neoplasm found in these fish. The preneoplastic lesions include basophilic foci, eosinophilic foci, and clear-cell foci. Cytotoxic lesions observed were fatty change, focal necrosis, hyaline degeneration of hepatocytes, and fatty change in pancreatic acinar cells. These preliminary results suggest the presence of carcinogens in Bayou Trepagnier

  7. Amazon Molly, Poecilia formosa, as a model for studies of the effects of ionizing radiation

    International Nuclear Information System (INIS)

    Woodhead, A.D.; Setlow, R.B.; Hart, R.W.

    1977-01-01

    It is suggested that the viviparous teleost, Poecilia formosa, (Amazon molly) may have wide potential use for aquatic radiation studies. The Amazon molly is a naturally occurring gynogenetic species, in which the eggs are activated after mating with the males of closely related species, without the subsequent genetic contribution from the male. The offspring of a single original female constitute a clone, having identical genotypes. Clones of the genetically homogeneous Amazon molly may prove to be equally as valuable to aquatic radiobiologists as the inbred rodent lines have been to mammalian studies. In many other respects the Amazon molly is a satisfactory laboratory animal. It is robust, easy to rear, and has large broods of young when fully grown. Maintenance costs are low. Details are given of the conditions under which colonies reproduce

  8. Quantifying the threat of extinction from Muller's ratchet in the diploid Amazon molly (Poecilia formosa

    Directory of Open Access Journals (Sweden)

    Loewe Laurence

    2008-03-01

    Full Text Available Abstract Background The Amazon molly (Poecilia formosa is a small unisexual fish that has been suspected of being threatened by extinction from the stochastic accumulation of slightly deleterious mutations that is caused by Muller's ratchet in non-recombining populations. However, no detailed quantification of the extent of this threat is available. Results Here we quantify genomic decay in this fish by using a simple model of Muller's ratchet with the most realistic parameter combinations available employing the evolution@home global computing system. We also describe simple extensions of the standard model of Muller's ratchet that allow us to deal with selfing diploids, triploids and mitotic recombination. We show that Muller's ratchet creates a threat of extinction for the Amazon molly for many biologically realistic parameter combinations. In most cases, extinction is expected to occur within a time frame that is less than previous estimates of the age of the species, leading to a genomic decay paradox. Conclusion How then does the Amazon molly survive? Several biological processes could individually or in combination solve this genomic decay paradox, including paternal leakage of undamaged DNA from sexual sister species, compensatory mutations and many others. More research is needed to quantify the contribution of these potential solutions towards the survival of the Amazon molly and other (ancient asexual species.

  9. The development of hyaline-cell cartilage in the head of the black molly, Poecilia sphenops. Evidence for secondary cartilage in a teleost.

    OpenAIRE

    Benjamin, M

    1989-01-01

    The development of hyaline-cell cartilage attached to membrane (dentary, maxilla, nasal, lacrimal and cleithrum) and cartilage (basioccipital) bones has been studied in the viviparous black molly, Poecilia sphenops. Intramembranous ossification commences before the first appearance of hyaline cells. As hyaline-cell cartilage is densely cellular and as that attached to the dentary, maxilla and cleithrum develops from the periosteum of these membrane bones, it must be regarded as secondary cart...

  10. Allele-specific expression at the androgen receptor alpha gene in a hybrid unisexual fish, the Amazon molly (Poecilia formosa.

    Directory of Open Access Journals (Sweden)

    Fangjun Zhu

    Full Text Available The all-female Amazon molly (Poecilia formosa is the result of a hybridization of the Atlantic molly (P. mexicana and the sailfin molly (P. latipinna approximately 120,000 years ago. As a gynogenetic species, P. formosa needs to copulate with heterospecific males including males from one of its bisexual ancestral species. However, the sperm only triggers embryogenesis of the diploid eggs. The genetic information of the sperm donor typically will not contribute to the next generation of P. formosa. Hence, P. formosa possesses generally one allele from each of its ancestral species at any genetic locus. This raises the question whether both ancestral alleles are equally expressed in P. formosa. Allele-specific expression (ASE has been previously assessed in various organisms, e.g., human and fish, and ASE was found to be important in the context of phenotypic variability and disease. In this study, we utilized Real-Time PCR techniques to estimate ASE of the androgen receptor alpha (arα gene in several distinct tissues of Amazon mollies. We found an allelic bias favoring the maternal ancestor (P. mexicana allele in ovarian tissue. This allelic bias was not observed in the gill or the brain tissue. Sequencing of the promoter regions of both alleles revealed an association between an Indel in a known CpG island and differential expression. Future studies may reveal whether our observed cis-regulatory divergence is caused by an ovary-specific trans-regulatory element, preferentially activating the allele of the maternal ancestor.

  11. Microsatellites for the gynogenetic Amazon molly, Poecilia formosa ...

    Indian Academy of Sciences (India)

    of high value for studies on the clonal variability and ori- gin of triploid .... Microsatellites in Poecilia species. Table 1. (contd). Amplification in. Diagnostic for. Locus. P. formosa. P. mexicana. P. latipinna. Species. Ploidy levels. MSD25 m m m n n .... be used to easily determine the species origin of individu- als and ploidy ...

  12. Local adaptation and pronounced genetic differentiation in an extremophile fish, Poecilia mexicana, inhabiting a Mexican cave with toxic hydrogen sulphide.

    Science.gov (United States)

    Plath, M; Hauswaldt, J S; Moll, K; Tobler, M; García De León, F J; Schlupp, I; Tiedemann, R

    2007-03-01

    We investigated genetic differentiation and migration patterns in a small livebearing fish, Poecilia mexicana, inhabiting a sulfidic Mexican limestone cave (Cueva del Azufre). We examined fish from three different cave chambers, the sulfidic surface creek draining the cave (El Azufre) and a nearby surface creek without the toxic hydrogen sulphide (Arroyo Cristal). Using microsatellite analysis of 10 unlinked loci, we found pronounced genetic differentiation among the three major habitats: Arroyo Cristal, El Azufre and the cave. Genetic differentiation was also found within the cave between different pools. An estimation of first-generation migrants suggests that (i) migration is unidirectional, out of the cave, and (ii) migration among different cave chambers occurs to some extent. We investigated if the pattern of genetic differentiation is also reflected in a morphological trait, eye size. Relatively large eyes were found in surface habitats, small eyes in the anterior cave chambers, and the smallest eyes were detected in the innermost cave chamber (XIII). This pattern shows some congruence with a previously proposed morphocline in eye size. However, our data do not support the proposed mechanism for this morphocline, namely that it would be maintained by migration from both directions into the middle cave chambers. This would have led to an increased variance in eye size in the middle cave chambers, which we did not find. Restricted gene flow between the cave and the surface can be explained by local adaptations to extreme environmental conditions, namely H2S and absence of light. Within the cave system, habitat properties are patchy, and genetic differentiation between cave chambers despite migration could indicate local adaptation at an even smaller scale.

  13. Frozen F1's amidst a masterpiece of nature: new insights into the rare hybrid origin of gynogenesis in the Amazon molly (Poecilia formosa).

    Science.gov (United States)

    Rogers, S M; Vamosi, S M

    2010-12-01

    All-female 'species' of fish have been shown to be great models in ecological and evolutionary studies because of the insights they can provide into the origin and evolution of asexuality, the ecology of hybrids, associations between genotype and environment, and the maintenance of sex. Gynogenetic organisms that evolved from sexual ancestors, and combine the disadvantageous traits from sexuality and asexuality, have long baffled evolutionary biologists trying to understand their origin and persistence with their sympatric sexual counterparts. In this issue, a new study using an integrated molecular phylogenetic and classical genetic approach has uncovered compelling evidence regarding the obscure asexual origin of the Amazon molly, Poecilia formosa. By performing an extensive phylogeographic analysis, Stöck et al. (2010) provide evidence that the Amazon molly arose only once within its history, with monophyly being strongly supported by mitochondrial DNA and microsatellite analyses. This result, combined with an elaborate failed attempt to resynthesize the lineage, suggests that vertebrate gynogens such as the Amazon molly are not rare because they are at a disadvantage to their sexual counterparts, but because the genomic conditions under which they arise are rare. Organisms that apparently combine the disadvantages of both sexuality and asexuality remain difficult to understand from both an ecological and an evolutionary perspective, and Stöck et al. (2010) highlight several outstanding important questions. Nonetheless, given that we now have a better knowledge of the origin and history of this unique 'species', this should allow researchers to better understand how these frozen F1's can persist amidst the masterpiece of nature. © 2010 Blackwell Publishing Ltd.

  14. Toxic hydrogen sulfide and dark caves: phenotypic and genetic divergence across two abiotic environmental gradients in Poecilia mexicana.

    Science.gov (United States)

    Tobler, Michael; Dewitt, Thomas J; Schlupp, Ingo; García de León, Francisco J; Herrmann, Roger; Feulner, Philine G D; Tiedemann, Ralph; Plath, Martin

    2008-10-01

    Divergent natural selection drives evolutionary diversification. It creates phenotypic diversity by favoring developmental plasticity within populations or genetic differentiation and local adaptation among populations. We investigated phenotypic and genetic divergence in the livebearing fish Poecilia mexicana along two abiotic environmental gradients. These fish typically inhabit nonsulfidic surface rivers, but also colonized sulfidic and cave habitats. We assessed phenotypic variation among a factorial combination of habitat types using geometric and traditional morphometrics, and genetic divergence using quantitative and molecular genetic analyses. Fish in caves (sulfidic or not) exhibited reduced eyes and slender bodies. Fish from sulfidic habitats (surface or cave) exhibited larger heads and longer gill filaments. Common-garden rearing suggested that these morphological differences are partly heritable. Population genetic analyses using microsatellites as well as cytochrome b gene sequences indicate high population differentiation over small spatial scale and very low rates of gene flow, especially among different habitat types. This suggests that divergent environmental conditions constitute barriers to gene flow. Strong molecular divergence over short distances as well as phenotypic and quantitative genetic divergence across habitats in directions classic to fish ecomorphology suggest that divergent selection is structuring phenotypic variation in this system.

  15. The development of hyaline-cell cartilage in the head of the black molly, Poecilia sphenops. Evidence for secondary cartilage in a teleost.

    Science.gov (United States)

    Benjamin, M

    1989-01-01

    The development of hyaline-cell cartilage attached to membrane (dentary, maxilla, nasal, lacrimal and cleithrum) and cartilage (basioccipital) bones has been studied in the viviparous black molly, Poecilia sphenops. Intramembranous ossification commences before the first appearance of hyaline cells. As hyaline-cell cartilage is densely cellular and as that attached to the dentary, maxilla and cleithrum develops from the periosteum of these membrane bones, it must be regarded as secondary cartilage according to current concepts. It is also argued that the hyaline-cell cartilage attached to the perichondral bone of the basioccipital (a cartilage bone), could also be viewed as secondary. The status of the cartilage on the nasal and lacrimal bones is less clear, for it develops, at least in part, from mucochondroid (mucous connective) tissue. This is the first definitive report of secondary cartilage in any lower vertebrate. The tissue is therefore not restricted to birds and mammals as hitherto believed, and a multipotential periosteum must have arisen early in vertebrate evolution. Images Fig. 1 Fig. 6 Fig. 7 Fig. 8 Fig. 9 Fig. 10 Fig. 11 Fig. 12 Fig. 13 Fig. 14 PMID:2481666

  16. The effects of sodium perchlorate on the liver of Molly Fish ( Poecilia ...

    African Journals Online (AJOL)

    Adult male molly fishes were reared up to ten days in control water or in water containing sodium perchlorate at concentrations of 1, 5, 25 and 125 ppm. Remarkable steatosis, fibrosis, hyperemia and necrosis were distinguished in parallel with increasing sodium perchlorate concentrations. The striking cellular damages ...

  17. Colorful invasion in permissive Neotropical ecosystems: establishment of ornamental non-native poeciliids of the genera Poecilia/Xiphophorus (Cyprinodontiformes: Poeciliidae and management alternatives

    Directory of Open Access Journals (Sweden)

    André Lincoln Barroso Magalhães

    2017-03-01

    Full Text Available ABSTRACT Headwater creeks are environments susceptible to invasion by non-native fishes. We evaluated the reproduction of 22 populations of the non-native livebearers guppy Poecilia reticulata, black molly Poecilia sphenops, Yucatan molly Poecilia velifera, green swordtail Xiphophorus hellerii, southern platyfish Xiphophorus maculatus, and variable platyfish Xiphophorus variatus during an annual cycle in five headwater creeks located in the largest South American ornamental aquaculture center, Paraíba do Sul River basin, southeastern Brazil. With few exceptions, females of most species were found reproducing (stages 2, 3, 4 all year round in the creeks and gravid females of all species showed small sizes indicating stunting. Juveniles were frequent in all sites. The fecundity of the six poeciliids was always low in all periods. The sex ratio was biased for females in most species, both bimonthly as for the whole period. Water temperature, water level and rainfall were not significantly correlated with reproduction in any species. Therefore, most populations appeared well established. The pertinence of different management actions, such as devices to prevent fish escape, eradication with rotenone and research about negative effects on native species, is discussed in the light of current aquaculture practices in the region.

  18. Inner Ear Morphology in the Atlantic Molly Poecilia mexicana—First Detailed Microanatomical Study of the Inner Ear of a Cyprinodontiform Species

    Science.gov (United States)

    Schulz-Mirbach, Tanja; Heß, Martin; Plath, Martin

    2011-01-01

    Background Fishes show an amazing diversity in hearing abilities, inner ear structures, and otolith morphology. Inner ear morphology, however, has not yet been investigated in detail in any member of the diverse order Cyprinodontiformes. We, therefore, studied the inner ear of the cyprinodontiform freshwater fish Poecilia mexicana by analyzing the position of otoliths in situ, investigating the 3D structure of sensory epithelia, and examining the orientation patterns of ciliary bundles of the sensory hair cells, while combining μ-CT analyses, scanning electron microscopy, and immunocytochemical methods. P. mexicana occurs in different ecotypes, enabling us to study the intra-specific variability (on a qualitative basis) of fish from regular surface streams, and the Cueva del Azufre, a sulfidic cave in southern Mexico. Results The inner ear of Poecilia mexicana displays a combination of several remarkable features. The utricle is connected rostrally instead of dorso-rostrally to the saccule, and the macula sacculi, therefore, is very close to the utricle. Moreover, the macula sacculi possesses dorsal and ventral bulges. The two studied ecotypes of P. mexicana showed variation mainly in the shape and curvature of the macula lagenae, in the curvature of the macula sacculi, and in the thickness of the otolithic membrane. Conclusions Our study for the first time provides detailed insights into the auditory periphery of a cyprinodontiform inner ear and thus serves a basis—especially with regard to the application of 3D techniques—for further research on structure-function relationships of inner ears within the species-rich order Cyprinodontiformes. We suggest that other poeciliid taxa, or even other non-poeciliid cyprinodontiforms, may display similar inner ear morphologies as described here. PMID:22110746

  19. Inner ear morphology in the Atlantic molly Poecilia mexicana--first detailed microanatomical study of the inner ear of a cyprinodontiform species.

    Science.gov (United States)

    Schulz-Mirbach, Tanja; Hess, Martin; Plath, Martin

    2011-01-01

    Fishes show an amazing diversity in hearing abilities, inner ear structures, and otolith morphology. Inner ear morphology, however, has not yet been investigated in detail in any member of the diverse order Cyprinodontiformes. We, therefore, studied the inner ear of the cyprinodontiform freshwater fish Poecilia mexicana by analyzing the position of otoliths in situ, investigating the 3D structure of sensory epithelia, and examining the orientation patterns of ciliary bundles of the sensory hair cells, while combining μ-CT analyses, scanning electron microscopy, and immunocytochemical methods. P. mexicana occurs in different ecotypes, enabling us to study the intra-specific variability (on a qualitative basis) of fish from regular surface streams, and the Cueva del Azufre, a sulfidic cave in southern Mexico. The inner ear of Poecilia mexicana displays a combination of several remarkable features. The utricle is connected rostrally instead of dorso-rostrally to the saccule, and the macula sacculi, therefore, is very close to the utricle. Moreover, the macula sacculi possesses dorsal and ventral bulges. The two studied ecotypes of P. mexicana showed variation mainly in the shape and curvature of the macula lagenae, in the curvature of the macula sacculi, and in the thickness of the otolithic membrane. Our study for the first time provides detailed insights into the auditory periphery of a cyprinodontiform inner ear and thus serves a basis--especially with regard to the application of 3D techniques--for further research on structure-function relationships of inner ears within the species-rich order Cyprinodontiformes. We suggest that other poeciliid taxa, or even other non-poeciliid cyprinodontiforms, may display similar inner ear morphologies as described here.

  20. Brain nonapeptide and gonadal steroid responses to deprivation of heterosexual contact in the black molly

    OpenAIRE

    Kulczykowska, Ewa; Kalamarz-Kubiak, Hanna; Nietrzeba, Marta; Gozdowska, Magdalena

    2014-01-01

    ABSTRACT Fish may respond to different social situations with changes in both physiology and behaviour. A unique feature of fish is that social interactions between males and females strongly affect the sexual characteristics of individuals. Here we provide the first insight into the endocrine background of two phenomena that occur in mono-sex groups of the black molly (Poecilia sphenops): masculinization in females and same-sex sexual behaviour, manifested by gonopodial displays towards same...

  1. Ovarian structure and oogenesis of the extremophile viviparous teleost Poecilia mexicana (Poeciliidae) from an active sulfur spring cave in Southern Mexico.

    Science.gov (United States)

    Torres-Martínez, Aarón; Hernández-Franyutti, Arlette; Uribe, Mari Carmen; Contreras-Sánchez, Wilfrido Miguel

    2017-12-01

    The structure of the ovary and oogenesis of Poecilia mexicana from an active sulfur spring cave is documented. Poecilia mexicana is the only poeciliid adapted to a subterranean environment with high hydrogen sulfide levels and extreme hypoxic conditions. Twenty females were captured throughout one year at Cueva del Azufre, located in the State of Tabasco in Southern Mexico. Ovaries were processed with histological techniques. P. mexicana has a single, ovoid ovary with ovigerous lamella that project to the ovarian lumen. The ovarian wall presents abundant loose connective tissue, numerous melanomacrophage centers and large blood vessels, possibly associated with hypoxic conditions. The germinal epithelium bordering the ovarian lumen contains somatic and germ cells forming cell nests projecting into the stroma. P. mexicana stores sperm in ovarian folds associated with follicles at different developmental phases. Oogenesis in P. mexicana consisted of the following stages: (i) oogonial proliferation, (ii) chromatin nucleolus, (iii) primary growth, subdivided into: (a) one nucleolus, (b) multiple nucleoli, (c) droplet oils-cortical alveoli steps; (iv) secondary growth, subdivided in: (a) early secondary growth, (b) late secondary growth, and (c) full grown. Follicular atresia was present in all stages of follicular development; it was characterized by oocyte degeneration, where follicle cells hypertrophy and differentiate in phagocytes. The ovary and oogenesis are similar to these seen in other poeciliids, but we found frequent atretic follicles, melanomacrophage centers, reduced fecundity and increased of offspring size. © 2017 Wiley Periodicals, Inc.

  2. Sequencing and characterization of the guppy (Poecilia reticulata transcriptome

    Directory of Open Access Journals (Sweden)

    Rodd F Helen

    2011-04-01

    a congeneric species, the sailfin molly (Poecilia latipinna. Over 40% of reads from the sailfin molly sample aligned to the guppy transcriptome. Conclusions We show that next-generation sequencing provided a reliable and broad reference transcriptome. This resource allowed us to identify candidate gene variants, SNPs in coding regions, and sex-specific gene expression, and permitted quantitative analysis of differential gene expression.

  3. Effects of extreme habitat conditions on otolith morphology: a case study on extremophile live bearing fishes (Poecilia mexicana, P. sulphuraria).

    Science.gov (United States)

    Schulz-Mirbach, Tanja; Riesch, Rüdiger; García de León, Francisco J; Plath, Martin

    2011-12-01

    Our study was designed to evaluate if, and to what extent, restrictive environmental conditions affect otolith morphology. As a model, we chose two extremophile livebearing fishes: (i) Poecilia mexicana, a widespread species in various Mexican freshwater habitats, with locally adapted populations thriving in habitats characterized by the presence of one (or both) of the natural stressors hydrogen sulphide and darkness, and (ii) the closely related Poecilia sulphuraria living in a highly sulphidic habitat (Baños del Azufre). All three otolith types (lapilli, sagittae, and asterisci) of P. mexicana showed a decrease in size ranging from the non-sulphidic cave habitat (Cueva Luna Azufre), to non-sulphidic surface habitats, to the sulphidic cave (Cueva del Azufre), to sulphidic surface habitats (El Azufre), to P. sulphuraria. Although we found a distinct differentiation between ecotypes with respect to their otolith morphology, no clear-cut pattern of trait evolution along the two ecological gradients was discernible. Otoliths from extremophiles captured in the wild revealed only slight similarities to aberrant otoliths found in captive-bred fish. We therefore hypothesize that extremophile fishes have developed coping mechanisms enabling them to avoid aberrant otolith growth - an otherwise common phenomenon in fishes reared under stressful conditions. Copyright © 2011 Elsevier GmbH. All rights reserved.

  4. Brain nonapeptide and gonadal steroid responses to deprivation of heterosexual contact in the black molly

    Directory of Open Access Journals (Sweden)

    Ewa Kulczykowska

    2014-12-01

    Full Text Available Fish may respond to different social situations with changes in both physiology and behaviour. A unique feature of fish is that social interactions between males and females strongly affect the sexual characteristics of individuals. Here we provide the first insight into the endocrine background of two phenomena that occur in mono-sex groups of the black molly (Poecilia sphenops: masculinization in females and same-sex sexual behaviour, manifested by gonopodial displays towards same-sex tank mates and copulation attempts in males. In socially controlled situations, brain neurohormones impact phenotypic sex determination and sexual behaviour. Among these hormones are the nonapeptides arginine vasotocin (AVT and isotocin (IT, counterparts of the well-known mammalian arginine vasopressin and oxytocin, respectively. To reveal potential hormone interactions, we measured the concentrations of bioactive AVT and IT in the brain, along with those of the sex steroids 17β-estradiol and 11-ketotestosterone in the gonads, of females, masculinized females, males displaying same-sex sexual behaviour and those who did not. These data were supplemented by morphological and histological analyses of the gonads. Correlations between brain nonapeptides and gonadal steroids strongly suggest a cross talk between hormonal systems. In the black molly, the masculinization process was associated with the production of brain AVT and gonadal steroids, whereas same-sex sexual behaviour involves both brain nonapeptides, but neither of the sex steroids. This study extends current knowledge of endocrine control of phenotypic sex and sexual behaviour in fish and for the first time links brain nonapeptides with the occurrence of male-male sexual behaviour in lower vertebrates.

  5. Life on the edge: hydrogen sulfide and the fish communities of a Mexican cave and surrounding waters.

    Science.gov (United States)

    Tobler, Michael; Schlupp, Ingo; Heubel, Katja U; Riesch, Rüdiger; de León, Francisco J García; Giere, Olav; Plath, Martin

    2006-12-01

    Most eucaryotic organisms classified as living in an extreme habitat are invertebrates. Here we report of a fish living in a Mexican cave (Cueva del Azufre) that is rich in highly toxic H(2)S. We compared the water chemistry and fish communities of the cave and several nearby surface streams. Our study revealed high concentrations of H(2)S in the cave and its outflow (El Azufre). The concentrations of H(2)S reach more than 300 muM inside the cave, which are acutely toxic for most fishes. In both sulfidic habitats, the diversity of fishes was heavily reduced, and Poecilia mexicana was the dominant species indicating that the presence of H(2)S has an all-or-none effect, permitting only few species to survive in sulfidic habitats. Compared to habitats without H(2)S, P. mexicana from the cave and the outflow have a significantly lower body condition. Although there are microhabitats with varying concentrations of H(2)S within the cave, we could not find a higher fish density in areas with lower concentrations of H(2)S. We discuss that P. mexicana is one of the few extremophile vertebrates. Our study supports the idea that extreme habitats lead to an impoverished species diversity.

  6. The virtual lover: variable and easily guided 3D fish animations as an innovative tool in mate-choice experiments with sailfin mollies-II. Validation.

    Science.gov (United States)

    Gierszewski, Stefanie; Müller, Klaus; Smielik, Ievgen; Hütwohl, Jan-Marco; Kuhnert, Klaus-Dieter; Witte, Klaudia

    2017-02-01

    The use of computer animation in behavioral research is a state-of-the-art method for designing and presenting animated animals to live test animals. The major advantages of computer animations are: (1) the creation of animated animal stimuli with high variability of morphology and even behavior; (2) animated stimuli provide highly standardized, controlled and repeatable testing procedures; and (3) they allow a reduction in the number of live test animals regarding the 3Rs principle. But the use of animated animals should be attended by a thorough validation for each test species to verify that behavior measured with live animals toward virtual animals can also be expected with natural stimuli. Here we present results on the validation of a custom-made simulation for animated 3D sailfin mollies Poecilia latipinna and show that responses of live test females were as strong to an animated fish as to a video or a live male fish. Movement of an animated stimulus was important but female response was stronger toward a swimming 3D fish stimulus than to a "swimming" box. Moreover, male test fish were able to discriminate between animated male and female stimuli; hence, rendering the animated 3D fish a useful tool in mate-choice experiments with sailfin mollies.

  7. Katak (Molly).

    Science.gov (United States)

    Pope, Mary L.; And Others

    This story about a girl named Molly is a preprimer designed for children in bilingual Inupiat-English programs in the Alaskan villages of Ambler, Kobuk, Kiana, Noorvik, Selawik, and Shungnak. It follows the first preprimer, "Denny." Each page of text is illustrated with a black-and-white drawing. The English equivalent is given at the…

  8. A new species of Poecilia from Honduras (Teleostei: Poeciliidae)

    NARCIS (Netherlands)

    Poeser, F.N.

    2011-01-01

    A new species of Poecilia from the Atlantic slopes of Honduras is described and assigned to the subgenus Mollienesia. Poecilia hondurensis, new species, differs from all congeners in the combination of having tricuspid teeth on the inner of both jaws and lacking a prominent spine or hook on the

  9. MDMA (Ecstasy/Molly)

    Science.gov (United States)

    ... Molly often actually get other drugs such as synthetic cathinones ("bath salts") instead (see " Added Risk of MDMA "). Some people take MDMA in combination with other drugs such as alcohol or marijuana. How does MDMA affect the brain? MDMA increases ...

  10. The virtual lover: variable and easily guided 3D fish animations as an innovative tool in mate-choice experiments with sailfin mollies-I. Design and implementation.

    Science.gov (United States)

    Müller, Klaus; Smielik, Ievgen; Hütwohl, Jan-Marco; Gierszewski, Stefanie; Witte, Klaudia; Kuhnert, Klaus-Dieter

    2017-02-01

    Animal behavior researchers often face problems regarding standardization and reproducibility of their experiments. This has led to the partial substitution of live animals with artificial virtual stimuli. In addition to standardization and reproducibility, virtual stimuli open new options for researchers since they are easily changeable in morphology and appearance, and their behavior can be defined. In this article, a novel toolchain to conduct behavior experiments with fish is presented by a case study in sailfin mollies Poecilia latipinna . As the toolchain holds many different and novel features, it offers new possibilities for studies in behavioral animal research and promotes the standardization of experiments. The presented method includes options to design, animate, and present virtual stimuli to live fish. The designing tool offers an easy and user-friendly way to define size, coloration, and morphology of stimuli and moreover it is able to configure virtual stimuli randomly without any user influence. Furthermore, the toolchain brings a novel method to animate stimuli in a semiautomatic way with the help of a game controller. These created swimming paths can be applied to different stimuli in real time. A presentation tool combines models and swimming paths regarding formerly defined playlists, and presents the stimuli onto 2 screens. Experiments with live sailfin mollies validated the usage of the created virtual 3D fish models in mate-choice experiments.

  11. There's something about Molly: The underresearched yet popular powder form of ecstasy in the United States.

    Science.gov (United States)

    Palamar, Joseph J

    2017-01-01

    Molly has been the street name for powder or crystalline ecstasy (3,4-methylenedioxymethamphetamine [MDMA]) in the United States since the early 2000s; however, few studies have examined Molly use or included Molly in the definition of ecstasy/MDMA. Prevalence of self-reported ecstasy use is being underreported on surveys due to the lack of inclusion of "Molly," although Molly is often so adulterated with novel psychoactive substances such as synthetic cathinones ("bath salts") that the name "Molly" may no longer adequately represent ecstasy/MDMA. The author recommends that Molly use and Molly purity be further studied to more adequately inform prevention and harm reduction.

  12. Effects of Alchemilla mollis and Alchemilla persica on the wound healing process

    Directory of Open Access Journals (Sweden)

    Burçin Ergene Öz

    2016-09-01

    Full Text Available Alchemilla mollis, is used in traditional medicine for the treatment of wounds and excessive menstruation. Aqueous methanol extracts of A. mollis and A. persica were evaluated for wound healing acivity by using linear incision and circular excision wound models along with hydroxyproline estimation and histopathological analysis. Anti-inflammatory effect was determined according to Whittle method. The extracts prepared from the aerial parts of A. mollis and A. persica exerted significant wound healing activity with the tensile strength values of 39.3% and 33.3%, respectively, and with the contraction values of 51.4% and 43.5%, respectively. Hydroxyproline estimation and histopathological analysis also confirmed the results. The extracts of A. mollis and A. persica showed significant anti-inflammatory activity with the values of 30.6% and 26.6% respectively. These results showed that A. mollis and A. persica possess significant wound healing and anti-inflammatory activities.

  13. Female Choice Undermines the Emergence of Strong Sexual Isolation between Locally Adapted Populations of Atlantic Mollies (Poecilia mexicana

    Directory of Open Access Journals (Sweden)

    Claudia Zimmer

    2018-05-01

    Full Text Available Divergent selection between ecologically dissimilar habitats promotes local adaptation, which can lead to reproductive isolation (RI. Populations in the Poecilia mexicana species complex have independently adapted to toxic hydrogen sulfide and show varying degrees of RI. Here, we examined the variation in the mate choice component of prezygotic RI. Mate choice tests across drainages (with stimulus males from another drainage suggest that specific features of the males coupled with a general female preference for yellow color patterns explain the observed variation. Analyses of male body coloration identified the intensity of yellow fin coloration as a strong candidate to explain this pattern, and common-garden rearing suggested heritable population differences. Male sexual ornamentation apparently evolved differently across sulfide-adapted populations, for example because of differences in natural counterselection via predation. The ubiquitous preference for yellow color ornaments in poeciliid females likely undermines the emergence of strong RI, as female discrimination in favor of own males becomes weaker when yellow fin coloration in the respective sulfide ecotype increases. Our study illustrates the complexity of the (partly non-parallel pathways to divergence among replicated ecological gradients. We suggest that future work should identify the genomic loci involved in the pattern reported here, making use of the increasing genomic and transcriptomic datasets available for our study system.

  14. Comparison of updates to the Molly cow model to predict methane production from dairy cows fed pasture.

    Science.gov (United States)

    Gregorini, P; Beukes, P C; Hanigan, M D; Waghorn, G; Muetzel, S; McNamara, J P

    2013-08-01

    Molly is a deterministic, mechanistic, dynamic model representing the digestion, metabolism, and production of a dairy cow. This study compared the predictions of enteric methane production from the original version of Molly (MollyOrigin) and 2 new versions of Molly. Updated versions included new ruminal fiber digestive parameters and animal hormonal parameters (Molly84) and a revised version of digestive and ruminal parameters (Molly85), using 3 different ruminal volatile fatty acid (VFA) stoichiometry constructs to describe the VFA pattern and methane (CH4) production (g of CH4/d). The VFA stoichiometry constructs were the original forage and mixed-diet VFA constructs and a new VFA stoichiometry based on a more recent and larger set of data that includes lactate and valerate production, amylolytic and cellulolytic bacteria, as well as protozoal pools. The models' outputs were challenged using data from 16 dairy cattle 26 mo old [standard error of the mean (SEM)=1.7], 82 (SEM=8.7) d in milk, producing 17 (SEM=0.2) kg of milk/d, and fed fresh-cut ryegrass [dry matter intake=12.3 (SEM=0.3) kg of DM/d] in respiration chambers. Mean observed CH4 production was 266±5.6 SEM (g/d). Mean predicted values for CH4 production were 287 and 258 g/d for MollyOrigin without and with the new VFA construct. Model Molly84 predicted 295 and 288 g of CH4/d with and without the new VFA settings. Model Molly85 predicted the same CH4 production (276 g/d) with or without the new VFA construct. The incorporation of the new VFA construct did not consistently reduce the low prediction error across the versions of Molly evaluated in the present study. The improvements in the Molly versions from MollyOrigin to Molly84 to Molly85 resulted in a decrease in mean square prediction error from 8.6 to 8.3 to 4.3% using the forage diet setting. The majority of the mean square prediction error was apportioned to random bias (e.g., 43, 65, and 70% in MollyOrigin, Molly84, and Molly85, respectively, on

  15. Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

    Directory of Open Access Journals (Sweden)

    Cássia A. O. Feres

    Full Text Available Dimorphandra mollis Benth., Fabaceae, also known as "faveira" or "fava-d'anta", is a plant common to the central woodsy meadow region of Brazil. It is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. Its principal component is rutin. The objective of this study is the evaluation of the safety of the use of the dried D. mollis extract in rodents. The rutin content of the standardized extract was 76.0±3%. With respect to the biochemical and hematological parameters evaluated, no alterations in the groups of rats that received 1000 and 2000 mg/kg doses of D. mollis were observed, but an increase in eosinophiles occurred. Hyperactivity of the white splenic pulp was detected in the group that received the 2000 mg/kg dose of D. mollis. In the evaluation of the lymphproliferative response with 1000 and 2000 mg/kg, no alterations were observed, and a decrease in IgG was only observed in the studies with a 2000 mg/kg dose. The results obtained with rodents suggest that no toxicity exists with the administration of dried D. mollis extract in a 1000 mg/kg dose.

  16. Effect of Heating on the Viscosity and Rheology of Grewia mollis ...

    African Journals Online (AJOL)

    This study determined the effect of concentration and prolonged heating on the rheological properties of gums from Grewia mollis, Bombax ceiba and their binary mixtures. After extraction, the gums were divided into four batches each. The binary mixture was obtained by triturating Grewia mollis and Bombax ceiba gum in a ...

  17. There’s Something About Molly: The Under-Researched yet Popular Powder Form of Ecstasy in the United States

    Science.gov (United States)

    Palamar, Joseph J.

    2017-01-01

    Molly has been the street name for powder or crystalline ecstasy (3,4-methylenedioxymethamphetamine [MDMA]) in the United States since at least 2008; however, few studies have examined Molly use or included Molly in the definition of ecstasy/MDMA. Prevalence of self-reported ecstasy use is being underreported on surveys due to the lack of inclusion of “Molly”, although Molly is often so adulterated with novel psychoactive substances such as synthetic cathinones (“bath salts”) that the name “Molly” may no longer adequately represent ecstasy/MDMA. The author recommends that Molly use and Molly purity be further studied to more adequately inform prevention and harm reduction. PMID:27925866

  18. Toxicological Response of Poecilia reticulata, Hyla species and ...

    African Journals Online (AJOL)

    PROF HORSFALL

    response of Poecilia reticulata, Hyla and Culex species was investigated using acute and chronic toxicity tests. ... responses over a 28 day period. .... sample. A control with only de-chlorinated tap water ..... landfills using luminescent bacteria.

  19. Influence of Tribulus terrestris on testicular enzyme in fresh water ornamental fish Poecilia latipinna.

    Science.gov (United States)

    Kavitha, P; Subramanian, P

    2011-12-01

    The influence of Tribulus terrestris on the activities of testicular enzyme in Poecilia latipinna was assessed in lieu of reproductive manipulation. Different concentrations of (100, 150, 200, 250, and 300 mg) Tribulus terrestris extract and of a control were tested for testicular activity of enzymes in Poecilia latipinna for 2 months. The testis and liver were homogenized separately in 0.1 mol/l potassium phosphate buffer (0.1 mol/l, pH 7.2). The crude homogenate was centrifuged, and supernatant obtained was used as an enzyme extract for determination of activities. The activities of testicular functional enzyme ALP, ACP, SDH, LDH, and G6PDH levels were changed to different extent in treated groups compared with that of the control. The total body weight and testis weight were increased with the Tribulus terrestris-treated fish (Poecilia latipinna). These results suggest that Tribulus terrestris induced the testicular enzyme activity that may aid in the male reproductive functions. It is discernible from the present study that Tribulus terrestris has the inducing effect on reproductive system of Poecilia latipinna.

  20. Acute toxicity of trichlorofon on four viviparous fish: Poecilia latipinna, Poecilia reticulata, Gambusia holbrooki and Xiphophorus helleri (Cyprinodontiformes: Poecilidae

    Directory of Open Access Journals (Sweden)

    Mohammad Forouhar Vajargah

    2014-07-01

    Full Text Available Objective: To determine toxicity and safety margins of trichlorofon in different species as trichlorofon toxicity has a large variation in the susceptibility of different species. Methods: In this research, four viviparous aquarium fish were exposed to trichlorofon for 96 h. LC50 of 24 h, 48 h, 72 h and 96 h were attained by probit analysis software SPSS Version 16. Fish samples were exposed to different concentrations of trichlorofon (5, 10, 20 and 40 mg/L for 96 h and mortality were recorded, separately. Results: The 96 h LC50 of Poecilia latipinna, Poecilia reticulata, Gambusia holbrooki and Xiphophorus helleri were 9.80, 9.80, 9.95 and 7.99 mg/L, respectively. Conclusions: According to the results of this research, LC50 values indicated Xiphophorus helleri was the most resistant and Gambusia holbrooki, with a few differences, was the most sensitive species compared with the fishes examined.

  1. Physicochemical Properties and Fatty Acid Profiles of Elaeagnus mollis Diels Nut Oils.

    Science.gov (United States)

    Liang, Shaohua; Yang, Ruinan; Dong, Caiwen; Yang, Qingping

    2015-01-01

    The physicochemical properties, fatty acid profiles, content of tocopherol and sterol of the oils extracted from the nuts of Elaeagnus mollis Diels grown in different regions of China were studied in this work. The results indicated that the Elaeagnus mollis Diels nut oils contained about 0.2% sterols and the tocopherol contents were in the range of 119.6-128.6mg/100g. The nut oils were all rich in unsaturated fatty acids, especially oleic acid and linoleic acid. Furthermore, the main triacylglycerols species of the nut oils were all dilinoleoyl-monoolein (LOL), dioleoyl-monolinoleoyl (OLO) and trilinoleate (LLL). This work might be useful for developing applications for Elaeagnus mollis Diels nut oil.

  2. Trophic relationships of small nonnative fishes in a natural creek and several agricultural drains flowing into the Salton Sea, and their potential, effects on the endangered desert pupfish

    Science.gov (United States)

    Martin, Barbara A.; Saiki, Michael K.

    2009-01-01

    This study was conducted to characterize trophic relationships of small nonnative fishes and to determine if predation by these fishes contributes to the decline of desert pupfish (Cyprinodon macularius), an endangered cyprinodont on the verge of extinction. We sampled 403 hybrid Mozambique tilapias (Oreochromis mossambica by O. urolepis), 107 redbelly tilapias (Tilapia zillii), 32 longjaw mudsuckers (Gillkhthys mirabilis), 182 western mosquitofish (Gambusia affinis), 222 sailfin mollies (Poecilia latipinna), 63 shortfin mollies (Poecilia mexicana), and 235 porthole livebearers (Poecilurpsis gracilis) from a natural creek and four agricultural drains during September 1999- December 2001. Evidence of piscivory was in gastrointestinal contents of 14 hybrid Mozambique tilapias, 3 redbelly tilapias, 10 longjaw mudsuckers, 8 western mosquitofish, 2 sailfin mollies, and 8 porthole livebearers. Although digestion often was too advanced for identification of fishes consumed by nonnative fishes, remains of desert pupfish were in gastrointestinal contents of a longjaw mudsucker. Our findings, along with Field evidence from other studies that inverse relationships exist between abundances of desert pupfish and nonnative species, are consistent with the hypothesis that predation by nonnative species is contributing to decline of desert pupfish. We suspect that competitive interactions with nonnative fishes might also adversely affect abundance of desert pupfish.

  3. Identification of myocardial diffuse fibrosis by 11 heartbeat MOLLI T 1 mapping

    DEFF Research Database (Denmark)

    Vassiliou, Vassilios S; Wassilew, Katharina; Cameron, Donnie

    2017-01-01

    OBJECTIVES: Our objectives involved identifying whether repeated averaging in basal and mid left ventricular myocardial levels improves precision and correlation with collagen volume fraction for 11 heartbeat MOLLI T 1 mapping versus assessment at a single ventricular level. MATERIALS AND METHODS...... and ECV. To assess correlation of T 1 mapping with collagen volume fraction, a separate cohort of ten aortic stenosis patients scheduled to undergo surgery underwent one CMR scan with this 11 heartbeat MOLLI scheme, followed by intraoperative tru-cut myocardial biopsy. Six models of myocardial diffuse...... fibrosis assessment were established with incremental inclusion of imaging by averaging of the basal and mid-myocardial left ventricular levels, and each model was assessed for precision and correlation with collagen volume fraction. RESULTS: A model using 11 heart beat MOLLI imaging of two basal and two...

  4. [The analyses and identification of Flos rhododendri mollis and Flos chrysanthemi indici via infrared spectroscopy].

    Science.gov (United States)

    Jin, Zhe-Xiong; Wang, Yue; Zhou, Qun; Chen, Jian-Bo; Ma, Fang; Sun, Su-Qin

    2014-09-01

    In this study, major chemical components of Flos rhododendri mollis and Flos chrysanthemi indici were characterized using Fourier transform infrared spectroscopy (FTIR). For Flos rhododendri mollis, the bands at 1,648 and 1,543 cm(-1) were attributed to amide I and amide II , respectively, indicating that it contained proteins probably resulting in immunization. In case of Flos chrysanthemi indici, stretching vibration of C==O function group was responsible for the bands at 1,734 and 1,515 cm(-1), as a result of essential oils, lipids, etc. Since FTIR spectra of Flos rhododendri mollis and Flos chrysanthemi indici are almost identical and it is difficult to discriminate them, two-step identification was investigated via secondary derivative of the FTIR spectra. The bands at 1,656 and 1,515 cm(-1) corresponds to flavonoides in Flos rhododendri mollis and Flos chrysanthemi indici. In the secondary derivative of the FTIR spectrum of Flos chrysanthemi indici, characteristic bands of inulin were present at 1,163, 1,077, 1,026, 986 and 869 cm(-1), and therefore Flos chrysanthemi indici contained inulin as well. Tri-step identification was carried out for Flos rhododendri mollis and Flos chrysanthemi indici by means of comparing their 2D-IR correlation spectra in different wave number range. In the characteristic range of flavonoides (1,700-1,400 cm(-1)), Flos rhododendri mollis exhibited 3 obvious autopeaks, while 10 autopeaks were visualized in the 2D-IR correlation spectrum of Flos chrysanthemi indici Moreover, in the characteristic range of glucoside (1,250-900 cm(-1)), 10 and 9 autopeaks were present in the 2D-IR correlation spectra of Flos rhododendri mollis and Flos chrysanthemi indici, respectively. Therefore, the tri-step identification of FTIR is a time-saving; accurate, cost-saving and convenient method to effectively distinguish traditional Chinese medicines.

  5. Body size and allometric shape variation in the molly Poecilia vivipara along a gradient of salinity and predation.

    Science.gov (United States)

    Araújo, Márcio S; Perez, S Ivan; Magazoni, Maria Julia C; Petry, Ana C

    2014-12-04

    Phenotypic diversity among populations may result from divergent natural selection acting directly on traits or via correlated responses to changes in other traits. One of the most frequent patterns of correlated response is the proportional change in the dimensions of anatomical traits associated with changes in growth or absolute size, known as allometry. Livebearing fishes subject to predation gradients have been shown to repeatedly evolve larger caudal peduncles and smaller cranial regions under high predation regimes. Poecilia vivipara is a livebearing fish commonly found in coastal lagoons in the north of the state of Rio de Janeiro, Brazil. Similar to what is observed in other predation gradients, lagoons inhabited by P. vivipara vary in the presence of piscivorous fishes; contrary to other poeciliid systems, populations of P. vivipara vary greatly in body size, which opens the possibility of strong allometric effects on shape variation. Here we investigated body shape diversification among six populations of P. vivipara along a predation gradient and its relationship with allometric trajectories within and among populations. We found substantial body size variation and correlated shape changes among populations. Multivariate regression analysis showed that size variation among populations accounted for 66% of shape variation in females and 38% in males, suggesting that size is the most important dimension underlying shape variation among populations of P. vivipara in this system. Changes in the relative sizes of the caudal peduncle and cranial regions were only partly in line with predictions from divergent natural selection associated with predation regime. Our results suggest the possibility that adaptive shape variation among populations has been partly constrained by allometry in P. vivipara. Processes governing body size changes are therefore important in the diversification of this species. We conclude that in species characterized by substantial

  6. HISTOLOGICAL CHANGES IN POECILIA RETICULATA AFTER INTERACTION OF IONIZING RADIATION AND ZINC SULFID

    Directory of Open Access Journals (Sweden)

    Michaela Špalková

    2012-12-01

    Full Text Available In our experiment we have studied interaction of ionizing radiation and zinc at Poecilia reticulata. Fish were irradiated with a 20 Gy of gamma-rays. Zinc sulphate in concentration 25 mg.l-1 was added to water in aquarium. Food intake, clinicl symptoms and histological changes were followed after gamma-irradiation and zinc sulfid in guppy Poecilia reticulata. In the first days timidity and lethargy were observed. The most prominent clinical symptoms observed were emaciation, hampered breathing and haemorrhages. Histological findings corresponded with these symptoms.doi:10.5219/228

  7. Effects of mechanical wounding on essential oil composition and emission of volatiles from Minthostachys mollis.

    Science.gov (United States)

    Banchio, Erika; Zygadlo, Julio; Valladares, Graciela R

    2005-04-01

    Plant tissues may show chemical changes following damage. This possibility was analyzed for Minthostachys mollis, a Lamiaceae native to Central Argentina with medicinal and aromatic uses in the region. Effects of mechanical damage on its two dominant monoterpenes, pulegone and menthone, were analyzed by perforating M. mollis leaves and then assessing essential oil composition at 24, 48, and 120 hr; emission of volatiles was also measured 24 and 48 hr after wounding. Mechanical damage resulted in an increase of pulegone and menthone concentration in M. mollis essential oil during the first 24 hr. These changes did not occur in the adjacent undamaged leaves, suggesting a lack of systemic response. Postwounding changes in the volatiles released from M. mollis damaged leaves were also detected, most noticeably showing an increase in the emission of pulegone. Inducible chemical changes in aromatic plants might be common and widespread, affecting the specific compounds on which commercial exploitation is based.

  8. Antibacterial activity of essential oil of Minthostachys mollis Griseb “RUYAQ MUÑA”

    OpenAIRE

    Carhuapoma Y., Mario; López G., Sofía; Roque A., Mirtha; Velapatiño, Billie; Bell C., Carlos; Whu W., Delia

    2014-01-01

    The aim of this study was to determine the antibacterial activity of essential oil Minthostachys mollis “ruyaq muña”, against Helicobacter pylori, Shigella dysenteriae, Salmonella typhi and Pseudomonas aeruginosa. The leaves of M. mollis were collected in the district of Huamanguilla (3000-3200 m.s.n.m), Huanta province, Ayacucho region. The essential oil obtained by distillation with water vapor drag. The antibacterial activity was determined by plate cultive excavation method, resulting in ...

  9. Clastic cave deposits in Botovskaya cave (Eastern Siberia, Russian Federation)

    Czech Academy of Sciences Publication Activity Database

    Kadlec, Jaroslav; Chadima, Martin; Lisá, Lenka; Hercman, H.; Osintsev, A.; Oberhänsli, H.

    2008-01-01

    Roč. 70, č. 3 (2008), s. 142-155 ISSN 1090-6924 Institutional research plan: CEZ:AV0Z30130516 Keywords : cave sediments * mineral magnetism * Botovskaya Cave Subject RIV: DE - Earth Magnetism, Geodesy, Geography Impact factor: 0.532, year: 2008 http://www.caves.org/pub/journal/Journal_of_Cave_and_Karst_Studies_volume_70.htm

  10. Comparing flow-through and static ice cave models for Shoshone Ice Cave

    Directory of Open Access Journals (Sweden)

    Kaj E. Williams

    2015-05-01

    Full Text Available In this paper we suggest a new ice cave type: the “flow-through” ice cave. In a flow-through ice cave external winds blow into the cave and wet cave walls chill the incoming air to the wet-bulb temperature, thereby achieving extra cooling of the cave air. We have investigated an ice cave in Idaho, located in a lava tube that is reported to have airflow through porous wet end-walls and could therefore be a flow-through cave. We have instrumented the site and collected data for one year. In order to determine the actual ice cave type present at Shoshone, we have constructed numerical models for static and flow-through caves (dynamic is not relevant here. The models are driven with exterior measurements of air temperature, relative humidity and wind speed. The model output is interior air temperature and relative humidity. We then compare the output of both models to the measured interior air temperatures and relative humidity. While both the flow-through and static cave models are capable of preserving ice year-round (a net zero or positive ice mass balance, both models show very different cave air temperature and relative humidity output. We find the empirical data support a hybrid model of the static and flow-through models: permitting a static ice cave to have incoming air chilled to the wet-bulb temperature fits the data best for the Shoshone Ice Cave.

  11. Pengaruh ukuran tubuh ikan Poecilia reticulata pada daya pemangsaannya terhadap larva Culex quinquefasciatus

    Directory of Open Access Journals (Sweden)

    Noer Moehammadi

    1999-06-01

    Full Text Available The research aimed to know the influence of poecilia reticulata body size, in the feeding capacity on Culex quinquifasciatus larvae. The research was experimental method with Complete Random Design., each treatment with 5 replications. The taken body size of Poecilia reticulata were; 1,50 cm; 2.50 cm; and 3.50 cm. the size of fish €™s body was measured from the tip of snout up to the end of fin (cm. The feeding capacity was determined by subtracting the first amount of larvae which was given the rest of larvae after 24 hours. The result showed that the difference of size of Poecilia reticulate body was significantly different in the feeding capacity on Culex quinquifasciatus larvae. The fish whose body size was 1.50 cm had the lowest feeding capacity by eating 77.2 larvae for average, and the highest one was the fist which body size was 2.50 cm by eating 113.6 larvae for average, subsequently the feeding capacity decrease to the fish which body size was 3.50 cm because of eating 100,6 larvae for 24 hours.

  12. Antimycobacterial activity of Juglans regia, Juglans mollis, Carya illinoensis and Bocconia frutescens.

    Science.gov (United States)

    Cruz-Vega, Delia Elva; Verde-Star, María Julia; Salinas-González, Noé; Rosales-Hernández, Bibiana; Estrada-García, Iris; Mendez-Aragón, Patricia; Carranza-Rosales, Pilar; González-Garza, María Teresa; Castro-Garza, Jorge

    2008-04-01

    Mycobacterium tuberculosis is a serious worldwide health threat, killing almost 2 million people per year. Alternative antimycobacterial drugs are urgently needed; studies have shown that medicinal plants traditionally used to treat respiratory diseases are a potential source of compounds to treat tuberculosis. This paper studied the antimycobacterial activity of 28 extracts from four different plant species that have been used in traditional Mexican medicine to treat tuberculosis. Bark and leaf crude extracts of Juglans regia L., Juglans mollis Engelm., Carya illinoensis (Wangenh) K. Koch and Bocconia frutescens showed in vitro anti-M. tuberculosis activity. Hexane bark extracts from C. illinoensis, J. mollis and J. regia were the most active with a minimal inhibitory concentration (MIC) of 31, 50 and 100 microg/mL, respectively. Ethanol bark extracts from C. illinoensis and J. mollis showed activity at 100 and 125 microg/mL, respectively. Leaf extracts had the lowest activity. Methanol and hexane leaves extracts from B. frutescens had a MIC of 125 microg/mL. None of the aqueous extracts showed antimycobacterial activity. (c) 2008 John Wiley & Sons, Ltd.

  13. Biostimulant on faveiro (Dimorphandra mollis Benth. seeds and seedling vigor

    Directory of Open Access Journals (Sweden)

    Tathiana Elisa Masetto

    2012-06-01

    Full Text Available This work aimed to verify the effects of biostimulant doses, applied through seeds, on faveiro (Dimorphandra mollis Benth. seeds and seedlings vigor. Seeds were previously sulfuric acid scarified in two consecutive years experiments. On 2006 August (first experiment seeds were submitted on Stimulate®: 0; 3.5; 7.0; 10.5 and 14 mL.0.5 Kg-1 doses (first experiment; and seeds picked in August, 2007, were Stimulate:0; 15; 20 and 25 mL.0.5Kg-1 of seeds treated (second experiment. After the biostimulant treatment seeds were sowed in cells trays containing 1:1 (v:v distroferric red latosol + plantmax®. The effect of different Stimulate® doses on Dimorphandra mollis seeds collected in different years were evaluated on the emergency percentage, speed emergency index, root length, aerial part height and dry seedling weight. It was conducted on entirely casualized with four repetitions of 25 seeds each treatment. The 14 mL (2006 lot and 15 mL (2007 lot 0.5Kg-1 of seeds doses provided larger percentage (50% and 66%, respectively and speed emergency indexes (0.67 and 0.9 respectively. The 20 mL.0.5Kg-1 of seeds dose treatment favored the aerial part length, but it didn't influenced the other Dimorphandra mollis seedlings vigor indexes.

  14. Food and Feeding Habits of the Guppy, Poecilia reticulata , from ...

    African Journals Online (AJOL)

    The food and feeding habits of the Guppy, Poecilia reticulata, from drainage canal systems in Lagos Metropolis, Nigeria, was investigated over a period of 24 months. Fish samples were collected monthly from 15 study sites. A total of 2400 fish stomachs were analyzed using the numerical and frequency of occurrence ...

  15. Cave age determination

    International Nuclear Information System (INIS)

    Anon.

    1998-01-01

    To determine the age of a cave we have to determine the age of elements which were created at the same moment when the cave was. Usually a cave is dug out by infiltration of acid waters. During this alteration process rocks free some aluminium and potassium ions. In Carlsbad and Lechuguilla caves, scientists have found alunite, this aluminium and potassium sulfate can be dated by using the carbon method of age determination. (A.C.)

  16. Salt ingestion caves.

    Directory of Open Access Journals (Sweden)

    Lundquist Charles A.

    2006-01-01

    Full Text Available Large vertebrate herbivores, when they find a salt-bearing layer of rock, say in a cliff face, can produce sizable voids where, overgenerations, they have removed and consumed salty rock. The cavities formed by this natural animal process constitute a uniqueclass of caves that can be called salt ingestion caves. Several examples of such caves are described in various publications. Anexample in Mississippi U.S.A., Rock House Cave, was visited by the authors in 2000. It seems to have been formed by deer orbison. Perhaps the most spectacular example is Kitum Cave in Kenya. This cave has been excavated to a length over 100 metersby elephants. An ancient example is La Cueva del Milodon in Chile, which is reported to have been excavated by the now extinctmilodon, a giant ground sloth. Still other possible examples can be cited. This class of caves deserves a careful definition. First, thecavity in rock should meet the size and other conventions of the locally accepted definition of a cave. Of course this requirement differsin detail from country to country, particularly in the matter of size. The intent is to respect the local conventions. The characteristicthat human entry is possible is judged to be a crucial property of any recognized cave definition. Second, the cavity should besignificantly the result of vertebrate animal consumption of salt-bearing rock. The defining process is that rock removed to form thecave is carried away in the digestive track of an animal. While sodium salts are expected to be the norm, other salts for which thereis animal hunger are acceptable. Also some other speleogenesis process, such as solution, should not be excluded as long as it issecondary in formation of a cave in question.

  17. Cryogenic Cave Pearls In the Periglacial Zones of Ice Caves

    Czech Academy of Sciences Publication Activity Database

    Žák, Karel; Orvošová, M.; Filippi, Michal; Vlček, M.; Onac, B. P.; Persoiu, A.; Rohovec, Jan; Světlík, Ivo

    2013-01-01

    Roč. 83, č. 2 (2013), s. 207-220 ISSN 1527-1404 R&D Projects: GA ČR GAP210/10/1760 Institutional research plan: CEZ:AV0Z30130516 Institutional support: RVO:67985831 ; RVO:61389005 Keywords : caves * cryogenic caves * ice caves * periglacial zones Subject RIV: DB - Geology ; Mineralogy Impact factor: 1.943, year: 2013

  18. Cardiac MOLLI T1 mapping at 3.0 T: comparison of patient-adaptive dual-source RF and conventional RF transmission.

    Science.gov (United States)

    Rasper, Michael; Nadjiri, Jonathan; Sträter, Alexandra S; Settles, Marcus; Laugwitz, Karl-Ludwig; Rummeny, Ernst J; Huber, Armin M

    2017-06-01

    To prospectively compare image quality and myocardial T 1 relaxation times of modified Look-Locker inversion recovery (MOLLI) imaging at 3.0 T (T) acquired with patient-adaptive dual-source (DS) and conventional single-source (SS) radiofrequency (RF) transmission. Pre- and post-contrast MOLLI T 1 mapping using SS and DS was acquired in 27 patients. Patient wise and segment wise analysis of T 1 times was performed. The correlation of DS MOLLI measurements with a reference spin echo sequence was analysed in phantom experiments. DS MOLLI imaging reduced T 1 standard deviation in 14 out of 16 myocardial segments (87.5%). Significant reduction of T 1 variance could be obtained in 7 segments (43.8%). DS significantly reduced myocardial T 1 variance in 16 out of 25 patients (64.0%). With conventional RF transmission, dielectric shading artefacts occurred in six patients causing diagnostic uncertainty. No according artefacts were found on DS images. DS image findings were in accordance with conventional T 1 mapping and late gadolinium enhancement (LGE) imaging. Phantom experiments demonstrated good correlation of myocardial T 1 time between DS MOLLI and spin echo imaging. Dual-source RF transmission enhances myocardial T 1 homogeneity in MOLLI imaging at 3.0 T. The reduction of signal inhomogeneities and artefacts due to dielectric shading is likely to enhance diagnostic confidence.

  19. Effect of Temperature on Reproduction and Sex Ratio of Guppy (Poecilia reticulata Peters

    Directory of Open Access Journals (Sweden)

    H. Arfah

    2007-01-01

    Full Text Available Water temperature could affect the reproduction of broodstock and sex ratio of progeny.  In this study, broodstock of guppy (Poecilia reticulata Peters was reared in different temperature to determine its effect on reproduction of broodstock and sex ratio of their progeny. The result of study show that broodstock reared at 27°C produced more fry (16 males mean than that of 30°C (10 males, while broodstock reared at 33°C produced no progeny.  Percentage of male fish produced by broodstock reared at 30°C is higher than that of 27°C.  Incubation time of embryo before birth is sorter in broodstock reared at 30°C (4-12 days compared with 27°C (18-22 days.  However, several progeny of broodstock reared at 30°C had abnormal vertebrae. Keywords: guppy, Poecilia reticulata, sex reversal, reproduction, monosex   ABSTRAK Suhu air inkubasi diduga dapat mempengaruhi reproduksi induk ikan dan nisbah kelamin keturunannya.  Pada penelitian ini, induk ikan gapi (Poecilia reticulata Peters dipelihara pada suhu 27°C, 30°C dan suhu 33°C untuk mengetahui pengaruhnya terhadap reproduksi dan rasio kelamin keturunannya.  Hasil penelitian menunjukkan bahwa induk ikan gapi yang dipelihara pada suhu 27°C menghasilkan anak lebih banyak (rata-rata 16 ekor daripada di suhu 30°C (10 ekor, sementara induk gagal melahirkan pada suhu pemeliharaan 33°C. Proporsi anak jantan yang dihasilkan oleh induk yang dipelihara pada suhu 30°C lebih banyak dibandingkan pada suhu 27°C.  Waktu inkubasi embrio sebelum dilahirkan oleh induk yang dipelihara pada suhu 30°C lebih singkat, yaitu 4-12 hari, dibandingkan pada  suhu 27°C, 18-22 hari.  Namun demikian beberapa anak ikan yang lahir dari induk yang dipelihara pada suhu suhu 30°C mengalami abnormalitas pada bagian tulang belakangnya.  Kata kunci: ikan gapi, Poecilia reticulata, sex reversal, reproduksi, monoseks

  20. Cytogenetic and molecular identification of a wheat-Leymus mollis alien multiple substitution line from octoploid Tritileymus x Triticum durum.

    Science.gov (United States)

    Pang, Y H; Zhao, J X; Du, W L; Li, Y L; Wang, J; Wang, L M; Wu, J; Cheng, X N; Yang, Q H; Chen, X H

    2014-05-23

    Leymus mollis (Trin.) Pilger (NsNsXmXm, 2n = 28), a wild relative of common wheat, possesses many traits that are potentially valuable for wheat improvement. In order to exploit and utilize the useful genes of L. mollis, we developed a multiple alien substitution line, 10DM50, from the progenies of octoploid Tritileymus M842-16 x Triticum durum cv. D4286. Genomic in situ hybridization analysis of mitosis and meiosis (metaphase I), using labeled total DNA of Psathyrostachys huashanica as probe, showed that the substitution line 10DM50 was a cytogenetically stable alien substitution line with 36 chromosomes from wheat and three pairs of Ns genome chromosomes from L. mollis. Simple sequence repeat analysis showed that the chromosomes 3D, 6D, and 7D were absent in 10DM50. Expressed sequence tag-sequence tagged sites analysis showed that new chromatin from 3Ns, 6Ns, and 7Ns of L. mollis were detected in 10DM50. We deduced that the substitution line 10DM50 was a multiple alien substitution line with the 3D, 6D, and 7D chromosomes replaced by 3Ns, 6Ns, and 7Ns from L. mollis. 10DM50 showed high resistance to leaf rust and significantly improved spike length, spikes per plant, and kernels per spike, which are correlated with higher wheat yield. These results suggest that line 10DM50 could be used as intermediate material for transferring desirable traits from L. mollis into common wheat in breeding programs.

  1. Locally adapted fish populations maintain small-scale genetic differentiation despite perturbation by a catastrophic flood event.

    Science.gov (United States)

    Plath, Martin; Hermann, Bernd; Schröder, Christiane; Riesch, Rüdiger; Tobler, Michael; García de León, Francisco J; Schlupp, Ingo; Tiedemann, Ralph

    2010-08-23

    Local adaptation to divergent environmental conditions can promote population genetic differentiation even in the absence of geographic barriers and hence, lead to speciation. Perturbations by catastrophic events, however, can distort such parapatric ecological speciation processes. Here, we asked whether an exceptionally strong flood led to homogenization of gene pools among locally adapted populations of the Atlantic molly (Poecilia mexicana, Poeciliidae) in the Cueva del Azufre system in southern Mexico, where two strong environmental selection factors (darkness within caves and/or presence of toxic H2S in sulfidic springs) drive the diversification of P. mexicana. Nine nuclear microsatellites as well as heritable female life history traits (both as a proxy for quantitative genetics and for trait divergence) were used as markers to compare genetic differentiation, genetic diversity, and especially population mixing (immigration and emigration) before and after the flood. Habitat type (i.e., non-sulfidic surface, sulfidic surface, or sulfidic cave), but not geographic distance was the major predictor of genetic differentiation. Before and after the flood, each habitat type harbored a genetically distinct population. Only a weak signal of individual dislocation among ecologically divergent habitat types was uncovered (with the exception of slightly increased dislocation from the Cueva del Azufre into the sulfidic creek, El Azufre). By contrast, several lines of evidence are indicative of increased flood-induced dislocation within the same habitat type, e.g., between different cave chambers of the Cueva del Azufre. The virtual absence of individual dislocation among ecologically different habitat types indicates strong natural selection against migrants. Thus, our current study exemplifies that ecological speciation in this and other systems, in which extreme environmental factors drive speciation, may be little affected by temporary perturbations, as adaptations

  2. Effects of Minthostachys mollis essential oil and volatiles on seedlings of lettuce, tomato, cucumbre and Bidens pilosa.

    OpenAIRE

    Alonso Amelot, Miguel; Usubillaga, Alfredo; Avila Nuñez, Jorge Luis; Oliveros Bastidas, Alberto; Avendaño Meza, Marisabel

    2007-01-01

    Effects of Minthostachys mollis essential oil and volatiles on seedlings of lettuce, tomato, cucumbre and Bidens pilosa. (Alonso Amelot, Miguel; Usubillaga, Alfredo; Avila Nuñez, Jorge Luis; Oliveros Bastidas, Alberto y Avendaño Meza, Mairsabel) Abstract The extraction and chemical composition of essential oil of Minthostachys mollis (Kunth) Griseb (Lamiaceae) and its inhibitory effects on germation and shoot/root elongation of lettuce, tomato, cucumber and Bidens pilosa (L...

  3. Cryogenic cave carbonates from the Cold Wind Cave, Nízke Tatry Mountains, Slovakia: Extending the age range of cryogenic cave carbonate formation to the Saalian

    Directory of Open Access Journals (Sweden)

    Zak K.

    2009-07-01

    Full Text Available Cold Wind Cave, located at elevations ranging between 1,600 and 1,700 m a. s. l. in the main range of the Nízke Tatry Mountains(Slovakia, is linked in origin with the adjacent Dead Bats Cave. Together, these caves form a major cave system located within anarrow tectonic slice of Triassic sediments. Both caves have undergone complex multiphase development. A system of sub-horizontalcave levels characterized by large, tunnel-like corridors was formed during the Tertiary, when elevation differences surroundingthe cave were less pronounced than today. The central part of the Nízke Tatry Mountains, together with the cave systems, wasuplifted during the Neogene and Lower Pleistocene, which changed the drainage pattern of the area completely. The formation ofnumerous steep-sloped vadose channels and widespread cave roof frost shattering characterized cave development throughout theQuaternary.In the Cold Wind Cave, extensive accumulations of loose, morphologically variable crystal aggregates of secondary cave carbonateranging in size between less than 1 mm to about 35 mm was found on the surface of fallen limestone blocks. Based on the C andO stable isotope compositions of the carbonate (δ13C: 0.72 to 6.34 ‰, δ18O: –22.61 to –13.68 ‰ V-PDB and the negative relationbetween δ13C and δ18O, the carbonate crystal aggregates are interpreted as being cryogenic cave carbonate (CCC. Publishedmodels suggest the formation of CCC in slowly freezing water pools, probably on the surface of cave ice, most probably duringtransitions from stadials to interstadials. Though the formation of these carbonates is likely one of the youngest events in thesequence of formation of cave sediments of the studied caves, the 230Th/234U ages of three samples (79.7±2.3, 104.0±2.9, and180.0±6.3 ka are the oldest so far obtained for CCC in Central Europe. This is the first description of CCC formation in one caveduring two glacial periods (Saalian and Weichselian.

  4. [Prediction of the suitable distribution and responses to climate change of Elaeagnus mollis in Shanxi Province, China].

    Science.gov (United States)

    Zhang, Yin Bo; Gao, Chen Hong; Qin, Hao

    2018-04-01

    Understanding the responses of the habitats of endangered species to climate change is of great significance for biodiversity conservation and the maintenance of the integrity of ecosystem function. In this study, the potential suitable distribution habitats of Elaeagnus mollis in Shanxi Province was simulated by the maximum entropy model, based on 73 occurrence field records and 35 environmental factors under the current climate condition. Moreover, with the Fifth Assessment Report of Intergovernmental Panel on Climate Change, the dynamics of distribution pattern was analyzed for E. mollis under different climate scenarios. The results showed that the area under the receiver operating characteristic curve (AUC) value was 0.987, indicating that the data fitted the model very well and that the prediction was highly reliable. Results from the Jackknife test showed that the main environmental variables affecting the E. mollis distribution were the precipitation seasonality, the range of annual temperature, annual mean temperature, isothermality, annual precipitation, and pH of topsoil, with the cumulative contribution reaching 94.8%. At present, the potential suitable habitats of E. mollis are mainly located in two regions, the southern of Lyuliang Mountain and Zhongtiao Mountain in Shanxi Province. Under different climate scenarios, the total suitable area of E. mollis would shrink in 2070s. In RCP 2.6 the suitable area would firstly increase and then decrease, while in RCP 4.5 and RCP 8.5 it would response sensitively and first decrease and then increase. Its spatial distribution in two suitable regions would show divergent responses to climate change. The distribution in southern Lyuliang Mountain would fluctuate slightly in latitudinal direction, while that in Zhongtiao Mountain would migrate along elevation.

  5. Experience-Dependent Color Constancy in Guppies (Poecilia reticulata

    Directory of Open Access Journals (Sweden)

    I. E. Intskirveli

    2002-01-01

    Full Text Available We investigated the ability to recognize the color of surfaces in fish (Poecilia reticulata, bred from birth in conditions of artificial light with constant spectral content. The capacity for color constancy significantly deteriorated when compared that to the control group. Further alteration of lighting conditions and transfer into natural daylight conditions restored the suppressed function to its normal level. We suggest that the color constancy function belongs in the visual system-response functions, the full development of which requires the accumulation of individual visual experience.

  6. Črna Jama as a cold air trap cave within Postojna Cave, Slovenia

    Science.gov (United States)

    Šebela, Stanka; Turk, Janez

    2017-10-01

    Črna Jama is the coldest section of cave within the Postojna Cave System. Mean annual air temperatures at the Črna Jama 2 site are 5.6 °C (2015) and 5.7 °C (2016), and at the Črna Jama 3 site 7.1 °C (2015) and 7.2 (2016), whereas the mean external air temperature was 10.3 °C (2015) and 10.0 °C (2016). In Lepe Jame, the passage most heavily visited by tourists, the mean cave-air temperature is 10.7 °C (2014-2017). Črna Jama exhibits winter and summer temperature regimes. During warm periods (Tcave Tout), ventilation takes place and dense, cold, outside air sinks into Črna Jama because of the favourable cave entrance morphology. Recent Črna Jama air temperature data (2014-2017) indicate a < 0.5 °C higher temperature than that recorded in historical data since 1933. Črna Jama is the most appropriate place within the Postojna Cave System to study long-term climatic changes. There are hardly any tourist visits to the cave, and human impacts on the cave climate are essentially reduced.

  7. Genetically uniform strains of fish as laboratory models for experimental studies of the effects of ionizing radiation

    International Nuclear Information System (INIS)

    Woodhead, A.D.; Setlow, R.B.; Hart, R.W.

    1979-01-01

    The advantages are discussed of using a genetically uniform test animal such as the amazon molly, Poecilia formosa, to reduce the biotic variability in experimental determination of the effects of ionizing radiation on aquatic ecosystems. Besides a cost reduction from using less organisms for these radiation-effect studies, another significant advantage of the higher precision responses due to homozygous genetic material is the assessment of radiation effects at the molecular and cellular levels. (author)

  8. EFFECT OF LIGHT AND GIBERELLIC ACID (AG3 ON THE GERMINATION OF MINTHOSTACHYS MOLLIS (LABIATAE

    Directory of Open Access Journals (Sweden)

    Diego Fernando Suárez Peñaranda

    2011-05-01

    Full Text Available Minthostachys mollis (Kunth Griseb. o muña es un arbusto perenne que crece naturalmente en las región andina de Colombia; esta especie es ampliamente utilizada por las comunidades rurales en donde es valorada por sus propiedades medicinales para tratar diferentes afecciones y como control de larvas de insectos transmisores de enfermedades; sin embargo, las poblaciones naturales de esta planta han sido fuertemente reducidas por acción antrópica, por lo tanto, es necesario generar tecnologías de propagación que permitan la recuperación y conservación de las poblaciones naturales. El objetivo de este estudio fue determinar el efecto de la luz (luz roja, luz blanca, luz natural y del ácido giberélico (0, 5, 15, 25 ppm en el proceso de germinación de M. mollis. Se encontró un efecto negativo de la oscuridad en el proceso de germinación y no se observó efecto del AG3 sobre la germinación de M. mollis en presencia de la luz. Los porcentajes de germinación obtenidos en los tratamientos de iluminación fueron superiores al 80% sobresaliendo el tratamiento de luz roja que arrojó los mejores resultados tanto en el porcentaje como en la velocidad de germinación. Los resultados indican que la germinación en M. mollis es dependiente de la luz debido a que sus semillas son fotoblásticas positivas.

  9. Who is 'Molly'? MDMA adulterants by product name and the impact of harm-reduction services at raves.

    Science.gov (United States)

    Saleemi, Sarah; Pennybaker, Steven J; Wooldridge, Missi; Johnson, Matthew W

    2017-08-01

    Methylenedioxymethamphetamine (MDMA), often sold as 'Ecstasy' or 'Molly', is commonly used at music festivals and reported to be responsible for an increase in deaths over the last decade. Ecstasy is often adulterated and contains compounds that increase morbidity and mortality. While users and clinicians commonly assume that products sold as Molly are less-adulterated MDMA products, this has not been tested. Additionally, while pill-testing services are sometimes available at raves, the assumption that these services decrease risky drug use has not been studied. This study analyzed data collected by the pill-testing organization, DanceSafe, from events across the United States from 2010 to 2015. Colorimetric reagent assays identified MDMA in only 60% of the 529 samples collected. No significant difference in the percentage of samples testing positive for MDMA was determined between Ecstasy and Molly. Individuals were significantly less likely to report intent to use a product if testing did not identify MDMA (relative risk (RR) = 0.56, p = 0.01). Results suggest that Molly is not a less-adulterated substance, and that pill-testing services are a legitimate harm-reduction service that decreases intent to consume potentially dangerous substances and may warrant consideration by legislators for legal protection. Future research should further examine the direct effects of pill-testing services and include more extensive pill-testing methods.

  10. Cave Entrance dependent Spore Dispersion of Filamentous Fungi Isolated from Various Sediments of Iron Ore Cave in Brazil: a colloquy on human threats while caving

    Directory of Open Access Journals (Sweden)

    Erika Linzi Silva Taylor

    2014-04-01

    Full Text Available Caves are stable environments with characteristics favoring the development of fungi. The fungal community present in a cave also includes pathogenic and opportunistic species out of which some are also served as energy sources in such energy stared ecosystems. Studies on microbial diversity and their role on such energy starved ecosystem are scarce. The present study was aimed to identify the cultivable filamentous fungi present in the various sediment of an iron ore cave and to recognize them as pathogenic and/or opportunistic species. Further the impact of cave entrance on the spore depositions on various distances dependent sediments were analyzed. The results suggest a diverse microbial community inhabiting the cave and an influence of cave entrance over spore deposition on various sediments. We counted a total of 4,549 filamentous fungi that included 34 species of 12 genera: Acremonium, Aspergillus, Cladosporium, Fusarium, Geotrichum, Paecilomyces, Purpureocillium, Penicillium, Torula, Trichoderma, Mucor and Rhizopus. A positive significant relation was observed between spore deposition and distance from cave entrance (p= 0.001. Areas of potential mycoses risks were recognized. This is the first study on microbiological community of an iron ore cave in the country.

  11. The show cave of Diros vs. wild caves of Peloponnese, Greece - distribution patterns of Cyanobacteria

    Directory of Open Access Journals (Sweden)

    Vasiliki Lamprinou

    2014-09-01

    Full Text Available The karst cave ‘Vlychada’of Diros, one of the oldest show caves in Peloponnese, sustains extended phototrophic biofilms on various substrata – on rocks inside the cave including speleothems, and especially near the artificial lighting installation (‘Lampenflora’. After a survey of the main abiotic parameters (Photosynthetically Active Radiation -PAR, Temperature -T, Relative Humidity -RH, Carbon Dioxide -CO2 three clusters of sampling sites were revealed according to Principal Component Analysis (PCA: i the water gallery section predominately influenced by CO2, ii the dry passages influenced by RH and PAR, and iii the area by the cave exit at the dry section influenced by temperature. The collected samples from the water gallery section and the dry passages of the cave revealed a total of 43 taxa of Cyanobacteria, with the unicellular/colonial forms being the most abundant. The applied non-metric Multi-dimensional Scaling Ordination (nMDS of the cumulative species composition showed a clear distinction between the water gallery section and the dry passages of the cave. Further comparison with previous data from other wild caves of Peloponnese (‘Kastria’, ‘Francthi’, and ‘Selinitsa’ was conducted revealing a distinction between the show cave and the wild ones. Apart from the human impact on cave ecosystems – through aesthetic alteration (‘greening’ of cave decorations by the ‘Lampenflora’, and by the cleaning treatments and restoration projects on the speleothems – identification of the organisms constituting the ‘Lampenflora’ might provide taxonomically and ecologically significant taxa.

  12. Estudo do sistema de reprodução da fava-d'anta (Dimorphandra mollis Benth.

    Directory of Open Access Journals (Sweden)

    A.D.R. Mendes

    2013-01-01

    Full Text Available Entre as plantas nativas de uso medicinal do Cerrado brasileiro encontra-se a fava-d'anta (Dimorphandra mollis Benth com alto potencial econômico por possuir inúmeras potencialidades medicinais e fitoquímicas. A indústria extrai dos frutos os princípios ativos rutina, quercetina, e ramnose, dentre outros, usados na fabricação de medicamentos e cosméticos, principalmente no exterior. O conhecimento do sistema reprodutivo da espécie é fundamental para sua conservação e manejo. O presente trabalho objetivou determinar as características morfométricas das flores e caracterizar o sistema reprodutivo da D. mollis em área de Cerrado inalterada antropomorficamente, no município de Olhos D'água - MG. Para as características morfométricas, diâmetro da flor, comprimento da flor, do ovário, e da antera, foram utilizadas cinco flores em pré-antese. Para a determinação do sistema reprodutivo utilizou-se a razão pólen:óvulo (P:O, em 40 flores. As flores da D. mollis apresentaram comprimento da flor de 3,00 mm, diâmetro da flor de 2,00 mm, comprimento do óvulo de 2,60 mm, comprimento da antera de 1,57 mm, e o número de óvulos e de anteras foram 20 e 5,8, respectivamente. A razão P:O foi 765,030, sendo que esse índice não é afetado pelas características morfométricas. O sistema reprodutivo da D. mollis foi classificado como alógama facultativa.

  13. Comparative study of infection with Tetrahymena of different ornamental fish species

    DEFF Research Database (Denmark)

    Sharon, G.; Leibowitz, M. Pimenta; Chettri, Jiwan Kumar

    2014-01-01

    different super orders. The species examined were platy (Xiphophorus), molly (Poecilia sphenops) and angelfish (Pterophyllum scalare) of the Acanthopterygii super order (which also includes guppies) and goldfish (Carassius auratus auratus) and koi carp (Cyprinus carpio) of the Ostariophysi super order....... These two super orders are phylogenetically distant from each other. Infection with Tetrahymena resulted in parasite invasion of internal organs, skin and muscle in all fish species. A relatively strong inflammatory response was observed in infected goldfish and koi, with negligible response in fish species...... of the Acanthopterygii super order. Guppies were the most susceptible to Tetrahymena infection, exhibiting a mortality rate of 87% and 100% in two separate experiments. A high mortality rate was also observed in platy (77%), while that of molly and angelfish was significantly lower (23% and 33%, respectively). Goldfish...

  14. Survey and hydrogeology of Carroll Cave

    Science.gov (United States)

    Carroll Cave, located in Camden County, Missouri, is the largest known cave formed in the Gasconade Dolomite of the Salem Plateau. Despite extensive visitation over the last 50 years and multiple survey efforts, a comprehensive map of the cave has never been produced. In 2002, the Carroll Cave Conse...

  15. The importance of ants in cave ecology, with new records and behavioral observations of ants in Arizona caves

    Directory of Open Access Journals (Sweden)

    Robert B. Pape

    2016-09-01

    Full Text Available The importance of ants as elements in cave ecology has been mostly unrecognized. A global list of ant species recorded from caves, compiled from a review of existing literature, is presented. This paper also reviews what is currently known about ants occurring in Arizona (USA caves. The diversity and distribution represented in these records suggests ants are relatively common cave visitors (trogloxenes. A general utilization of caves by ants within both temperate and tropical latitudes may be inferred from this combined evidence. Observations of ant behavior in Arizona caves demonstrate a low level and sporadic, but persistent, use of these habitats and their contained resources by individual ant colonies. Documentation of Neivamyrmex sp. preying on cave-inhabiting arthropods is reported here for the first time. Observations of hypogeic army ants in caves suggests they may not penetrate to great vertical depth in search of prey, but can be persistent occupants in relatively shallow, horizontal sections of caves where they may prey on endemic cave animals. First cave records for ten ant species are reported from Arizona caves. These include two species of Neivamyrmex (N. nigrescens Cresson and Neivamyrmex sp.; Formicidae: Dorylinae, four myrmicines (Pheidole portalensis Wilson, Pheidole cf. porcula Wheeler, Solenopsis aurea Wheeler and Stenamma sp. Westwood, one dolichoderine (Forelius keiferi Wheeler and three formicines (Lasius arizonicus Wheeler, L. sitiens Wilson, and Camponotus sp. Mayr.

  16. Exposure to radon in Cuban tourist caves

    International Nuclear Information System (INIS)

    Carrazana Gonzalez, Jorge; Dominguez Ley, Orlando; Gil Castillo, Reinaldo; Molerio Leon, Leslie

    2008-01-01

    With the objective of estimating the dose, due to Radon-222, received by tour guides and other people who work inside some of the most important tourist caves in the Republic of Cuba, measurements of radon concentrations were carried out in four of these caves: Santo Tomas Cave, Tapiada Cave, Del Indio Cave and Jose Miguel Cave. All these caves are located in the Vinales Valley (Pinar del Rio City), a very important tourist area in the country. The relationship among radon concentration, the concentration of natural radionuclides inside the caves and the geologic characteristics of the specific locations was analyzed. In order to select the most appropriates measurement places, different criteria were taken into account: representatives points of the geology of the caves, level of gamma radiation, exchange of air, places of work inside the caves (cafeterias, restaurants and shops) and places more frequently visited by tour guides. The maximum radon concentration was found in Jose Miguel Cave with a value of 220 Bq/m 3 . The measurement of gamma dose rate inside the caves was carried out with a scintillation detector type Scintrex BGS-3 previously calibrated at SSI, Sweden. The radon concentrations were measured with the equipment Alpha Guard PQ2000/MC50 and SARAD RM2000, both also calibrated at SSI, Sweden. The obtained results indicated that, in the studied caves, the annual dose is not greater than 1 mSv considering a work year of 2000 hours. (author)

  17. Fruit consumption and seed dispersal of Dimorphandra mollis Benth. (Leguminosae) by the lowland tapir in the cerrado of Central Brazil.

    Science.gov (United States)

    Bizerril, M X A; Rodrigues, F H G; Hass, A

    2005-08-01

    Fruit phenology observations and consumption of Dimorphandra mollis (Leguminosae) were analyzed during seven months in an area of cerrado stricto sensu. We analysed 81 fecal samples collected at six different places of lowland tapirs (Tapirus terrestris) in central Brazilian cerrado. In addition, from the feces of five tapirs at the Brasília Zoo to which fruit had been offered, seeds were collected and used in germination tests. The results suggest that the tapir is an important fruit consumer and a potential seed disperser of D. mollis. In the field, however, fruit consumption was found to be very low, probably because of both fruit palatability and the low density of frugivores, especially tapirs. The possibility that the original dispersal agents of D. mollis seeds belonged to the South American Pleistocene megafauna is discussed.

  18. Simulated oxygen isotopes in cave drip water and speleothem calcite in European caves

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    A. Wackerbarth

    2012-11-01

    Full Text Available Interpreting stable oxygen isotope (δ18O records from stalagmites is still one of the complex tasks in speleothem research. Here, we present a novel model-based approach, where we force a model describing the processes and modifications of δ18O from rain water to speleothem calcite (Oxygen isotope Drip water and Stalagmite Model – ODSM with the results of a state-of-the-art atmospheric general circulation model enhanced by explicit isotope diagnostics (ECHAM5-wiso. The approach is neither climate nor cave-specific and allows an integrated assessment of the influence of different varying climate variables, e.g. temperature and precipitation amount, on the isotopic composition of drip water and speleothem calcite.

    First, we apply and evaluate this new approach under present-day climate conditions using observational data from seven caves from different geographical regions in Europe. Each of these caves provides measured δ18O values of drip water and speleothem calcite to which we compare our simulated isotope values. For six of the seven caves modeled δ18O values of drip water and speleothem calcite are in good agreement with observed values. The mismatch of the remaining caves might be caused by the complexity of the cave system, beyond the parameterizations included in our cave model.

    We then examine the response of the cave system to mid-Holocene (6000 yr before present, 6 ka climate conditions by forcing the ODSM with ECHAM5-wiso results from 6 ka simulations. For a set of twelve European caves, we compare the modeled mid-Holocene-to-modern difference in speleothem calcite δ18O to available measurements. We show that the general European changes are simulated well. However, local discrepancies are found, and might be explained either by a too low model resolution, complex local soil-atmosphere interactions affecting evapotranspiration or by cave specific factors

  19. Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus mollis Lm#7Ns (7D Disomic Substitution Line with Stripe Rust Resistance.

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    Xiaofei Yang

    Full Text Available Leymus mollis (2n = 4x = 28, NsNsXmXm possesses novel and important genes for resistance against multi-fungal diseases. The development of new wheat-L. mollis introgression lines is of great significance for wheat disease resistance breeding. M11003-3-1-15-8, a novel disomic substitution line of common wheat cv. 7182 -L. mollis, developed and selected from the BC1F5 progeny between wheat cv. 7182 and octoploid Tritileymus M47 (2n = 8x = 56, AABBDDNsNs, was characterized by morphological and cytogenetic identification, analysis of functional molecular markers, genomic in situ hybridization (GISH, sequential fluorescence in situ hybridization (FISH-genomic in situ hybridization (GISH and disease resistance evaluation. Cytological observations suggested that M11003-3-1-15-8 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. The GISH investigations showed that line contained 40 wheat chromosomes and a pair of L. mollis chromosomes. EST-STS multiple loci markers and PLUG (PCR-based Landmark Unique Gene markers confirmed that the introduced L. mollis chromosomes belonged to homoeologous group 7, it was designated as Lm#7Ns. While nulli-tetrasomic and sequential FISH-GISH analysis using the oligonucleotide Oligo-pSc119.2 and Oligo-pTa535 as probes revealed that the wheat 7D chromosomes were absent in M11003-3-1-15-8. Therefore, it was deduced that M11003-3-1-15-8 was a wheat-L. mollis Lm#7Ns (7D disomic substitution line. Field disease resistance demonstrated that the introduced L. mollis chromosomes Lm#7Ns were responsible for the stripe rust resistance at the adult stage. Moreover, M11003-3-1-15-8 had a superior numbers of florets. The novel disomic substitution line M11003-3-1-15-8, could be exploited as an important genetic material in wheat resistance breeding programs and genetic resources.

  20. Chemical composition and antimicrobial activity of the essential oil of Plectranthus mollis (Lamiaceae from Western Ghats region, Karnataka, India

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    Rajesh K. Joshi

    2014-08-01

    Full Text Available Plectranthus is a large and widespread genus with a diversity of ethnobotanical uses. In traditional medicine P. mollis has been used against snakebites, respiratory stimulant and vasoconstrictor, cardiac depressant, cure for haemorrahage, treatment of mental retardation and rheumatism. P. mollis is reported to exhibit relaxant activity on smooth and skeletal muscles, and has cytotoxic and anti-tumour promoting activity, and can be used in the treatment of cancer. The aim of the present study was to identify chemical composition of the essential oil of P. mollis and to evaluate antimicrobial efficacy of the oil. The essential oil of the flowering aerial parts of P. mollis as obtained by hydro-distillation and analyzed by gas chromatography equipped with a flame ionization detector (GC-FID and gas chromatography coupled with mass spectrometry (GC/MS. Twentyseven compounds were identified, which comprised 98.6% of the total constituents. The main compound was identified as fenchone (32.3%, followed by α-humulene (17.3%, piperitenone oxide (8.5%, cis-piperitone oxide (6.0% and E-β-farnesene (5.9%. The oil was found rich in oxygenated monoterpenes type constituents (52.0%, followed by sesquiterpene hydrocarbons (40.2%, oxygenated sesquiterpenes (4.9%, and monoterpene hydrocarbons (1.5%. Antimicrobial activity of the essential oil of P. mollis was tested against six Gram-positive and eight Gram negative bacteria, and three fungi, by using the tube dilution method. The oil was active against the tested Gram-positive and Gram-negative bacteria, and fungi at a concentration range of 0.065±0.008-0.937±0.139mg/mL, 0.468±0.069-3.333±0.527 mg/mL and 0.117±0.0170.338±0.062mg/mL respectively. The present study revealed that the oil constituents somehow were qualitatively similar and quantitatively different than earlier reports from different parts of the world. The essential oil of P. mollis has found to be antimicrobial activity which can be

  1. Energy expenditure in caving.

    Directory of Open Access Journals (Sweden)

    Giorgia Antoni

    Full Text Available The aim of this study was to determine the energy expenditure of a group of cavers of both genders and different ages and experience during a 10 hour subterranean exploration, using portable metabolimeters. The impact of caving activity on body composition and hydration were also assessed through bioelectrical impedance, and nutritional habits of cavers surveyed. During cave activity, measured total energy expenditure (TEE was in the range 225-287 kcal/h for women-men (MET = 4.1, respectively; subjects had an energy intake from food in the range 1000-1200 kcal, thus inadequate to restore lost calories. Bayesian statistical analysis estimated the effect of predictive variables on TEE, revealing that experienced subjects had a 5% lower TEE than the less skilled ones and that women required a comparatively larger energy expenditure than men to perform the same task. BIVA (bioelectrical impedance vector analysis showed that subjects were within the range of normal hydration before and after cave activity, but bioelectrical changes indicated a reduction of extracellular water in men, which might result in hypo-osmolal dehydration in the case of prolonged underground exercise. All these facts should be considered when planning cave explorations, preparing training programs for subjects practising caving, and optimizing a diet for cavers. Further, information gathered through this study could be of value to reduce accidents in caves related to increase in fatigue.

  2. Investigation of clay sediments and bedrock morphology in caves with seismic traveltime tomography: an application at Alepotrypa Cave (Diros, Greece

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    Lazaros Polymenakos

    2017-01-01

    Full Text Available The deposition of unconsolidated clay sediments in caves, in relation to the buried morphology of the karstic conduit, are important parameters for the study of cave evolution. We introduce the application of an active seismic imaging technique to investigate the clay deposits and bedrock morphology in caves. Seismic traveltime tomography, applied for the first time in cave studies, can assist with the interpretation of cave geomorphology. Utilizing the P-wave velocity contrast between the clay sediments and the surrounding rock mass, we map the buried rock surface and significant sediment interfaces and provide an estimate of the sediment thickness and volume. Our study focuses on the Alepotrypa Cave located in Diros (Peloponnese, Greece, revealing important information for the evolution of the cave. The proposed technique could be applied in caves with significant clay deposits, in order to constrain the clay volume and reconstruct the buried floor shape of the cave. The technique exploits fully the ground morphology and access points in a cave, so it is suitable for a detailed three-dimensional exploration of cave deposits and the underlying cave morphology.

  3. GEOMORPHIC ANALYSIS OF MAWSMAI CAVE, MEGHALAYA, INDIA

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    Vibhash Chandra Jha

    2011-12-01

    Full Text Available ABSTRACT Caving is an art which can be best experienced in the mystic Mawsmai Cave of Meghalaya. This Cave is fast becoming a hot tourist’s destination offering great adventurous opportunity to the tourists. The cave is located in the East Khasi Hill region near Cherrapunjee and is made of limestone. The emotion of thrill heightens after entering the cave. The entrance has a narrow vertical opening and is well lit. Due to its location in the world’s largest rainfall region, dripping of water from the cave roofs occurs almost throughout the year. The formation of stalactites and stalagmites create wonderful phenomena specially found in this caves. The conspicuous pillars formed due to the joining of the roof and the floors are an awe-inspiring creativity of the creator of this world. Keywords: Cave, Limestone, Stalactite, Stalagmite, Pillar, Solution.

  4. Effect of Light and Giberellic Acid (AG3) on the Germination of Minthostachys mollis Kunth. Griseb. (Labiatae)

    OpenAIRE

    Suárez, Diego; Fernández Alonso, José Luis; Marina Melgarejo, Luz

    2011-01-01

    Minthostachys mollis (Kunth) Griseb., es un arbusto perenne que crece en la región andina de Colombia; es ampliamente utilizada por las comunidades rurales donde es valorado por sus propiedades medicinales. El objetivo de este estudio fue determinar el efecto de la luz (luz roja, luz blanca y luz natural) y del ácido giberélico (0, 5, 15, 25 ppm) en el proceso de germinación de M. mollis. Se encontró inhibición de la germinación bajo condiciones de oscuridad. No se observó efecto de AG3 sobre...

  5. Fruit consumption and seed ispersal of Dimorphandra mollis Benth. (Leguminosae by the lowland tapir in the Cerrado of Central Brazil

    Directory of Open Access Journals (Sweden)

    M. X. A. Bizerril

    Full Text Available Fruit phenology observations and consumption of Dimorphandra mollis (Leguminosae were analyzed during seven months in an area of cerrado stricto sensu. We analysed 81 fecal samples collected at six different places of lowland tapirs (Tapirus terrestris in central Brazilian cerrado. In addition, from the feces of five tapirs at the Brasília Zoo to which fruit had been offered, seeds were collected and used in germination tests. The results suggest that the tapir is an important fruit consumer and a potential seed disperser of D. mollis. In the field, however, fruit consumption was found to be very low, probably because of both fruit palatability and the low density of frugivores, especially tapirs. The possibility that the original dispersal agents of D. mollis seeds belonged to the South American Pleistocene megafauna is discussed.

  6. Simultaneous Detection of Flavonoids, Phenolic Acids and Alkaloids in Abri Herba and Abri Mollis Herba using Liquid Chromatography Tandem Mass Spectrometry.

    Science.gov (United States)

    Yan, Wenying; Han, Qingjie; Guo, Panpan; Wang, Chunying; Zhang, Zijian

    2016-01-01

    Abri Herba has remarkable properties, such as cleanup heat detoxification, dampness and activating blood circulation to dissipate blood stasis; as a result, it has been applied to treat acute or chronic hepatitis and mastitis. Abri mollis Herba is often used as Abri Herba. Hierarchical cluster analysis (HCA) was applied to compare the similarities and differences of the chemical compositions in the two types of medicinal materials. To establish a high-performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) method for the simultaneous analysis of 15 flavonoids, two phenolic acids and three alkaloids in Abri Herba and Abri mollis Herba. The chromatographic separation was performed on a C18 column with a mobile phase of methanol (A), acetonitrile (B) and 0.5‰ acetic acid in water (C) using gradient elution. The detection of the target compounds was performed in multiple-reaction monitoring (MRM) mode using a hybrid quadrupole linear ion trap mass spectrometer equipped with positive/negative ion-switching electrospray ionisation (ESI) source. The developed method is reliable, sensitive and specific. In addition, the method has been successfully applied to differentiate 15 batches of Abri Herba and 27 batches of Abri mollis Herba stems. Furthermore, a comparison of the contents among stems, roots and leaves from the same strain in seven batches of Abri mollis Herba and four batches of Abri Herba has also been performed. HPLC-MS/MS method is sensitive and selective and can be suitable for the reliable quality control of Abri mollis Herba and Abri Herba. Copyright © 2015 John Wiley & Sons, Ltd.

  7. EFECTO DE LA LUZ Y DEL ÁCIDO GIBERÉLICO (AG3) EN LA GERMINACIÓN DE Minthostachys mollis KUNTH. GRISEB.(LABIATAE) Effect of Light and Giberellic Acid (AG3) on the Germination of Minthostachys mollis Kunth. Griseb. (Labiatae)

    OpenAIRE

    DIEGO SUÁREZ; JOSÉ LUIS FERNÁNDEZ ALONSO; LUZ MARINA MELGAREJO

    2011-01-01

    Minthostachys mollis (Kunth) Griseb., es un arbusto perenne que crece en la región andina de Colombia; es ampliamente utilizada por las comunidades rurales donde es valorado por sus propiedades medicinales. El objetivo de este estudio fue determinar el efecto de la luz (luz roja, luz blanca y luz natural) y del ácido giberélico (0, 5, 15, 25 ppm) en el proceso de germinación de M. mollis. Se encontró inhibición de la germinación bajo condiciones de oscuridad. No se observó efecto de AG3 sobre...

  8. Isolation and molecular cytogenetic characterization of a wheat - Leymus mollis double monosomic addition line and its progenies with resistance to stripe rust.

    Science.gov (United States)

    Yang, Xiaofei; Li, Xin; Wang, Changyou; Chen, Chunhuan; Tian, Zengrong; Ji, Wanquan

    2017-12-01

    A common wheat - Leymus mollis (2n = 4x = 28, NsNsXmXm) double monosomic addition line, M11003-4-3-8/13/15 (2n = 44 = 42T.a + L.m2 + L.m3), with stripe rust resistance was developed (where T.a represents Triticum aestivum chromosome, L.m represents L. mollis chromosome, and L.m2/3 represents L. mollis chromosome of homoeologous groups 2 and 3). The progenies of line M11003-4-3-8/13/15 were characterized by cytological observation, specific molecular markers, fluorescence in situ hybridization (FISH), and genomic in situ hybridization (GISH). Among the progenies, there existed five different types (I, II, III, IV, and V) of chromosome constitution, the formulas of which were 2n = 44 = 42T.a + 1L.m2 + 1L.m3, 2n = 43 = 42T.a + 1L.m2, 2n = 43 = 42T.a + 1L.m3, 2n = 42 = 42T.a, and 2n = 44 = 42T.a + 2L.m2, respectively. Field disease screening showed that types I and III showed high resistance to stripe rust, while types II, IV, and V were susceptible. Leymus mollis was almost immune to stripe rust, whereas the wheat parent, cultivar 7182, was susceptible. Therefore, we concluded that the stripe rust resistance originated from L. mollis. These various lines could be further fully exploited as important disease resistance materials to enrich wheat genetic resources.

  9. Cave Tourism: The Potential of Asar Cave as a Natural Tourism Asset at Lenggong Valley, Perak

    Directory of Open Access Journals (Sweden)

    Rindam Main

    2014-01-01

    Full Text Available The Lenggong Valley, from a standpoint of natural tourism research, presents strengths, weaknesses, opportunities and challenges that can be utilized to help increase the opportunities for the local community to increase their standard of living. Asar Cave comprises one of the caves that are found in Lenggong. A series of external studies have been done on Asar Cave in order to measure its potential for natural tourism in Lenggong. The objective of this study is to discuss caves as a natural resource that has great potential in the growth of the economy of the residents of the Lenggong Valley. Marketing caves as a source of nature tourism helps the government’s achievements in National Key Result Areas, apart from being a form of environmental control as well as helping to increase awareness about environmental education, specifically those associated with caves. The research results find that SWOT analysis presents huge potential for caves to become a source of nature tourism development in Lenggong. Great potential can also be seen from a standpoint of increasing the standard of living of its residents through their involvement in the tourism sector based on local natural assets.

  10. The Mammoth Cave system, Kentucky, USA; El sistema de la Mammoth Cave, Kentucky, EE.UU

    Energy Technology Data Exchange (ETDEWEB)

    Palmer, A. N.

    2016-07-01

    Mammoth Cave is the main attraction of Mammoth Cave National Park. For several decades it has been the longest known cave in the world and currently contains 652 km in 2016 of surveyed passages. It is located in the heart of an extensive karst plateau, in which the stratal dip averages only one degree. The cave is part of a drainage basin of more than 200 km{sup 2}. The cave has been known to local inhabitants for several millennia and contains a rich trove of archaeological and historical artifacts. It contains many speleo biota including several rare and endangered species and has been designated a World Heritage Site and an International Biosphere Reserve (UNESCO). Its many passage levels and sediments contain a record of the fluvial history of most of south-eastern North America. (Author)

  11. Instrumenting caves to collect hydrologic and geochemical data: case study from James Cave, Virginia

    Science.gov (United States)

    Schreiber, Madeline E.; Schwartz, Benjamin F.; Orndorff, William; Doctor, Daniel H.; Eagle, Sarah D.; Gerst, Jonathan D.

    2015-01-01

    Karst aquifers are productive groundwater systems, supplying approximately 25 % of the world’s drinking water. Sustainable use of this critical water supply requires information about rates of recharge to karst aquifers. The overall goal of this project is to collect long-term, high-resolution hydrologic and geochemical datasets at James Cave, Virginia, to evaluate the quantity and quality of recharge to the karst system. To achieve this goal, the cave has been instrumented for continuous (10-min interval) measurement of the (1) temperature and rate of precipitation; (2) temperature, specific conductance, and rate of epikarst dripwater; (3) temperature of the cave air; and (4) temperature, conductivity, and discharge of the cave stream. Instrumentation has also been installed to collect both composite and grab samples of precipitation, soil water, the cave stream, and dripwater for geochemical analysis. This chapter provides detailed information about the instrumentation, data processing, and data management; shows examples of collected datasets; and discusses recommendations for other researchers interested in hydrologic and geochemical monitoring of cave systems. Results from the research, briefly described here and discussed in more detail in other publications, document a strong seasonality of the start of the recharge season, the extent of the recharge season, and the geochemistry of recharge.

  12. Petrographic and geochemical study on cave pearls from Kanaan Cave (Lebanon

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    Nader Fadi H.

    2007-01-01

    Full Text Available The Kanaan cave is situated at the coastal zone, north of Beirut City (capital of Lebanon. The cave is located within the upper part of the Jurassic Kesrouane Formation (Liassic to Oxfordian which consists mainly of micritic limestone. Twenty seven cave pearls were subjected to petrographic (conventional and scanning electron microscopy and geochemical analyses (major/trace elements and stable isotopes. The cave pearls were found in an agitated splash-pool with low mud content. They are believed to have formed through chemical precipitation of calcite in water over-saturated with calcium. The nucleus and micritic laminae show δ18OV-PDB values of about -5.0‰ and δ13C V-PDB values of -11.8‰, while the surrounding calcite spar laminae resulted in δ18OV-PDB ranging between -5.3 and -5.2‰, and δ13C V-PDB between -12.3 and -12.1‰. A genesis/diagenesis model for these speleothems is proposed involving recrystallization which has selectively affected the inner layers of the cave pearls. This is chiefly invoked by sparry calcite crystals ‘invading’ the inner micrite cortical laminae and the nuclei (cross-cutting the pre-existing mud-envelopes, and the slight depletion in δ18O values from inner to outer cortical layers. The calculated δ18OV-SMOW of the water (-4.2‰ matches with data on meteoric water signature for the central eastern Mediterranean region.

  13. Environmental conditions of Borra Cave, Visakhapattanam, India

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    Haraprasad Bairagya

    2014-05-01

    Full Text Available Caving is an art which can be best experienced in the mystic Borra of Eastern Ghats and ranked as the second largest cave of India just after Belum Caves situated in the same state Andhrapradesh, India. This Cave is fast becoming a hot tourist?s destination offering great adventurous opportunity to the tourists in the Eastern Ghats. The cave is located in the Ananthagiri hills of the Eastern Ghats region near Visakhapattanam and is made of limestone. The emotion of thrill heightens after entering the cave. The entrance has a narrow vertical opening and is well lit. Due to its location in the sub-equatorial region, dripping of water from the cave roofs occurs almost throughout the year. The formation of stalactites and stalagmites create wonderful phenomena specially found in this cave. The conspicuous pillars formed due to the joining of the roof and the floors are an awe-inspiring creativity of the creator of this world. Various viruses and bacteria are in the cave interior along with different other creatures. The Borra cave helps the Govt. of Andhra Pradesh, India, to earn huge economic benefits for the sake of tourism industry. DOI: http://dx.doi.org/10.3126/ije.v3i2.10526 International Journal of the Environment Vol.3(2 2014: 150-166

  14. Cave monitoring in the Béke and Baradla caves (Northeastern Hungary: implications for the conditions for the formation cave carbonates

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    György Czuppon

    2018-01-01

    Full Text Available In order to use speleothems in the reconstruction of past climate and environmental changes it is necessary to understand the environmental and hydrological processes that determine the physico-chemical conditions of carbonate precipitation and hence speleothem formation. Therefore, in this study an extended monitoring program was conducted in the Béke and Baradla caves located in the Aggtelek region (Northeastern Hungary. The studied caves are rich in speleothem and flowstone occurrences with great potential for paleoclimatology studies. The monitoring activity included measurements of atmospheric and cave temperatures, CO2 concentration in cave air, as well as chemical and isotopic compositions of water samples (drip water, precipitation and in situ carbonate precipitates. The hydrogen and oxygen isotope compositions of drip waters showed no seasonal variation at any of the collection sites, indicating a well-mixed karstic aquifer. This implies that the isotopic compositions of local speleothems were able to record multiannual isotopic changes inherited from stable isotopes in the drip water. CO2 concentration showed seasonality (high values in summer and low values in winter in both caves, likely affecting carbonate precipitation or corrosion and consequently stalagmite growth. Systematic variations among Mg/Ca and Sr/Ca, Na/Ca, and Si/Ca element ratios were detected in the drip water suggesting Prior Calcite Precipitation (PCP. As PCP is characteristic of periods of reduced infiltration during drier weather conditions, the variations in drip water chemistry and drip rates indicate that the hydrological conditions also varied significantly during the studied period. This hydrological variability appears to affect not only trace element composition but also the isotopic composition of modern carbonate precipitates. In summary, these findings imply that the speleothems from the studied caves were able to record the hydrological changes

  15. Spotted hyena and steppe lion predation behaviours on cave bears of Europe - ?Late Quaternary cave bear extinction as result of predator stress

    Science.gov (United States)

    Diedrich, Cajus G.

    2010-05-01

    Cave bears hibernated in caves all over Eurasia (e.g. Rabeder et al., 2000) including alpine regions using mainly larger caves for this purpose. Late Quaternary spotted hyenas Crocuta crocuta spelaea instead occupied mainly areas close to the cave entrances as their dens (Diedrich and Žák 2006, Diedrich 2010). The largest predator, the steppe lion Panthera leo spelaea was only a sporadic cave dweller (Diedrich 2007b, 2009b). His presence and its remains from caves all over Europe can be recently explained best as result of imported carcasses after killing by their largest antagonists, the Late Quaternary spotted hyenas. In some cases the kill might have happened in the hyena den cave itself during the theft of prey remains by lions (Diedrich 2009a). Another reason of their remains in caves of Europe is the hunting onto the herbivorous cave bears, especially during hibernation times, when megafauna prey was less available in the open environments (Diedrich 2009c). These lion remains from caves of Europe, nearly all of which were from adult animals, provide evidence of active predation by lions onto cave bears even in medium high alpine regions (Diedrich 2009b, in review). Lion skeletons in European cave bear dens were therefore often found amongst originally articulated cave bear skeletons or scattered cave bear remains and even close to their hibernation nests (Diedrich et al. 2009c, in review). Not only lions fed on cave bears documented mainly by the large quantities of chewed, punctured and crushed cave bear long-bones; even damaged skulls reveal that hyenas scavenged primarily on cave bear carcasses which were mainly responsible for the destruction of their carcasses and bones (Diedrich 2005, 2009d). Predation and scavenging on cave bears by the two largest Late Quaternary predators C. c. spelaea and P. l. spelaea explains well the large quantity of fragmented cave bear bones over all European caves in low to medium high mountainous elevations, whereas in

  16. Bacterial community survey of sediments at Naracoorte Caves, Australia

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    Ball Andrew S.

    2012-07-01

    Full Text Available Bacterial diversity in sediments at UNESCO World Heritage listed Naracoorte Caves was surveyed as part of an investigation carried out in a larger study on assessing microbial communities in caves. Cave selection was based on tourist accessibility; Stick Tomato and Alexandra Cave (> 15000 annual visits and Strawhaven Cave was used as control (no tourist access. Microbial analysis showed that Bacillus was the most commonly detected microbial genus by culture dependent and independent survey of tourist accessible and inaccessible areas of show (tourist accessible and control caves. Other detected sediment bacterial groups were assigned to the Firmicutes, Actinobacteria and Proteobacteria. The survey also showed differences in bacterial diversity in caves with human access compared to the control cave with the control cave having unique microbial sequences (Acinetobacter, Agromyces, Micrococcus and Streptomyces. The show caves had higher bacterial counts, different 16S rDNA based DGGE cluster patterns and principal component groupings compared to Strawhaven. Different factors such as human access, cave use and configurations could have been responsible for the differences observed in the bacterial community cluster patterns (tourist accessible and inaccessible areas of these caves. Cave sediments can therefore act as reservoirs of microorganisms. This might have some implications on cave conservation activities especially if these sediments harbor rock art degrading microorganisms in caves with rock art.

  17. Is radon emission in caves causing deletions in satellite DNA sequences of cave-dwelling crickets?

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    Giuliana Allegrucci

    Full Text Available The most stable isotope of radon, 222Rn, represents the major source of natural radioactivity in confined environments such as mines, caves and houses. In this study, we explored the possible radon-related effects on the genome of Dolichopoda cave crickets (Orthoptera, Rhaphidophoridae sampled in caves with different concentrations of radon. We analyzed specimens from ten populations belonging to two genetically closely related species, D. geniculata and D. laetitiae, and explored the possible association between the radioactivity dose and the level of genetic polymorphism in a specific family of satellite DNA (pDo500 satDNA. Radon concentration in the analyzed caves ranged from 221 to 26,000 Bq/m3. Specimens coming from caves with the highest radon concentration showed also the highest variability estimates in both species, and the increased sequence heterogeneity at pDo500 satDNA level can be explained as an effect of the mutation pressure induced by radon in cave. We discovered a specific category of nuclear DNA, the highly repetitive satellite DNA, where the effects of the exposure at high levels of radon-related ionizing radiation are detectable, suggesting that the satDNA sequences might be a valuable tool to disclose harmful effects also in other organisms exposed to high levels of radon concentration.

  18. Fish mitigate trophic depletion in marine cave ecosystems.

    Science.gov (United States)

    Bussotti, Simona; Di Franco, Antonio; Bianchi, Carlo Nike; Chevaldonné, Pierre; Egea, Lea; Fanelli, Emanuela; Lejeusne, Christophe; Musco, Luigi; Navarro-Barranco, Carlos; Pey, Alexis; Planes, Serge; Vieux-Ingrassia, Jean Vincent; Guidetti, Paolo

    2018-06-15

    Dark marine habitats are often characterized by a food-limited condition. Peculiar dark habitats include marine caves, characterized by the absence of light and limited water flow, which lead to reduced fluxes of organic matter for cave-dwelling organisms. We investigated whether the most abundant and common cave-dwelling fish Apogon imberbis has the potential to play the role of trophic vector in Mediterranean marine caves. We first analysed stomach contents to check whether repletion changes according to a nycthemeral cycle. We then identified the prey items, to see whether they belong to species associated with cave habitats or not. Finally, we assessed whether A. imberbis moves outside marine caves at night to feed, by collecting visual census data on A. imberbis density both inside and outside caves, by day and by night. The stomach repletion of individuals sampled early in the morning was significantly higher than later in the day. Most prey were typical of habitats other than caves. A. imberbis was on average more abundant within caves during the day and outside during the night. Our study supports the hypothesis regarding the crucial trophic role of A. imberbis in connecting Mediterranean marine caves with external habitats.

  19. Cave breakdown by vadose weathering.

    Directory of Open Access Journals (Sweden)

    Osborne R. Armstrong L.

    2002-01-01

    Full Text Available Vadose weathering is a significant mechanism for initiating breakdown in caves. Vadose weathering of ore bodies, mineral veins, palaeokarst deposits, non-carbonate keystones and impure, altered or fractured bedrock, which is intersected by caves, will frequently result in breakdown. Breakdown is an active, ongoing process. Breakdown occurs throughout the vadose zone, and is not restricted to large diameter passages, or to cave ceilings. The surfaces of disarticulated blocks are commonly coated, rather than having fresh broken faces, and blocks continue to disintegrate after separating from the bedrock. Not only gypsum, but also hydromagnesite and aragonite are responsible for crystal wedging. It is impossible to study or identify potential breakdown foci by surface surveys alone, in-cave observation and mapping are essential.

  20. The Mammoth Cave system, Kentucky, USA

    International Nuclear Information System (INIS)

    Palmer, A. N.

    2016-01-01

    Mammoth Cave is the main attraction of Mammoth Cave National Park. For several decades it has been the longest known cave in the world and currently contains 652 km in 2016 of surveyed passages. It is located in the heart of an extensive karst plateau, in which the stratal dip averages only one degree. The cave is part of a drainage basin of more than 200 km 2 . The cave has been known to local inhabitants for several millennia and contains a rich trove of archaeological and historical artifacts. It contains many speleo biota including several rare and endangered species and has been designated a World Heritage Site and an International Biosphere Reserve (UNESCO). Its many passage levels and sediments contain a record of the fluvial history of most of south-eastern North America. (Author)

  1. Solar activity influence on air temperature regimes in caves

    Science.gov (United States)

    Stoeva, Penka; Mikhalev, Alexander; Stoev, Alexey

    Cave atmospheres are generally included in the processes that happen in the external atmosphere as circulation of the cave air is connected with the most general circulation of the air in the earth’s atmosphere. Such isolated volumes as the air of caves are also influenced by the variations of solar activity. We discuss cave air temperature response to climate and solar and geomagnetic activity for four show caves in Bulgaria studied for a period of 46 years (1968 - 2013). Everyday noon measurements in Ledenika, Saeva dupka, Snezhanka and Uhlovitsa cave have been used. Temperatures of the air in the zone of constant temperatures (ZCT) are compared with surface temperatures recorded at meteorological stations situated near about the caves - in the towns of Vratsa, Lovech, Peshtera and Smolyan, respectively. For comparison, The Hansen cave, Middle cave and Timpanogos cave from the Timpanogos Cave National Monument, Utah, USA situated nearly at the same latitude have also been examined. Our study shows that the correlation between cave air temperature time series and sunspot number is better than that between the cave air temperature and Apmax indices; that t°ZCT is rather connected with the first peak in geomagnetic activity, which is associated with transient solar activity (CMEs) than with the second one, which is higher and connected with the recurrent high speed streams from coronal holes. Air temperatures of all examined show caves, except the Ledenika cave, which is ice cave show decreasing trends. On the contrary, measurements at the meteorological stations show increasing trends in the surface air temperatures. The trend is decreasing for the Timpanogos cave system, USA. The conclusion is that surface temperature trends depend on the climatic zone, in which the cave is situated, and there is no apparent relation between temperatures inside and outside the caves. We consider possible mechanism of solar cosmic rays influence on the air temperatures in caves

  2. Postglacial recolonization and the biogeography of Palmaria mollis (Rhodophyta) along the Northeast Pacific coast

    NARCIS (Netherlands)

    Lindstrom, S.C; Olsen, J.L.; Stam, W.T.

    1997-01-01

    We used random amplified polymorphic DNA (RAPD) markers to examine the distribution of genotypes of Palmaria mollis (Setchell et Gardner) van der Meer et Bird, a red alga. We sampled populations along the Northeast Pacific coast from northern Washington to southwestern Alaska, an area extensively

  3. Quantitative variations in the essential oil of Minthostachys mollis (Kunth.) Griseb. in response to insects with different feeding habits.

    Science.gov (United States)

    Banchio, Erika; Zygadlo, Julio; Valladares, Graciela R

    2005-08-24

    Plants display a diverse array of inducible changes in secondary metabolites following insect herbivory. Herbivores differ in their feeding behavior, physiology, and mode of attachment to the leaf surface, and such variations might be reflected in the induced responses of damaged plants. Induced changes were analyzed for Minthostachys mollis, a Lamiaceae with medicinal and aromatic uses, and four species of folivore insects with different feeding habits (chewing, scraping, sap-sucking, and puncturing). In M. mollis leaves experimentally exposed to the insects, levels of the two dominant monoterpenes pulegone and menthone were assessed 24 and 48 h after wounding. Menthone content generally decreased in the essential oil of damaged leaves, whereas pulegone concentration increased in all treatments. These changes occurred also in the adjacent undamaged leaves, suggesting a systemic response. The relatively uniform response to different kinds of damage could be attributable to the presence of such a strongly active compound as pulegone in the essential oil of M. mollis. The effects of wounding on essential oil concentration may be significant from a commercial point of view.

  4. Influence of GAMMA radiation on morphological changes of Poecilia reticulata

    International Nuclear Information System (INIS)

    Benova, K.; Cigankova, V.; Dvorak, P.; Hromada, R.

    2006-01-01

    In our experiment were followed histological changes after gamma-irradiation with dose of 30 Gy in Poecilia reticulata. After radiation shyness and lethargy were observed. The most prominent clinical symptoms observed were emaciation, hampered breathing, ex ophthalmia and hemorrhages. The histological picture found were adequate to these symptoms. The enteritic villi compared with controls were relatively low. Enterocytes taking part on resorption processes were damaged and desquamated on some sites, and the number of microvilli was reduced on their surface. As our earlier findings on rats revealed, the decrease in number of microvilli designates malfunctioning intestinal resorption, which can lead to emaciation. (authors)

  5. Effect of light and gibberellic acid (AG3) on the germination of minthostachys mollis kunth. Griseb. (labiatae)

    International Nuclear Information System (INIS)

    Suarez, Diego; Fernandez Alonso, Jose Luis; Melgarejo, Luz Marina

    2011-01-01

    Minthostachys mollis (Kunth) griseb, is a perennial shrub that grows in the Andean region of Colombia; this species is widely used by rural communities where it is valued for its medicinal properties. The aim of this study was to determine the effect of light (red light, white light, and natural light) and gibberellic acid (0, 5, 15, 25 ppm) on the process of germination of M. mollis. We found a strong inhibition of germination under conditions of darkness. We do not observe effect of AG3 on germination. The germination percentages obtained were above 80% (positive photoblastic seeds) for the three proposed lighting treatments where red-light treatment had a most significant effect on the germination process.

  6. World Register of marine Cave Species (WoRCS: a new Thematic Species Database for marine and anchialine cave biodiversity

    Directory of Open Access Journals (Sweden)

    Vasilis Gerovasileiou

    2016-09-01

    Full Text Available Scientific exploration of marine cave environments and anchialine ecosystems over recent decades has led to outstanding discoveries of novel taxa, increasing our knowledge of biodiversity. However, biological research on underwater caves has taken place only in a few areas of the world and relevant information remains fragmented in isolated publications and databases. This fragmentation makes assessing the conservation status of marine cave species especially problematic, and this issue should be addressed urgently given the stresses resulting from planned and rampant development in the coastal zone worldwide. The goal of the World Register of marine Cave Species (WoRCS initiative is to create a comprehensive taxonomic and ecological database of known species from marine caves and anchialine systems worldwide and to present this as a Thematic Species Database (TSD of the World Register of marine Species (WoRMS. WoRCS will incorporate ecological data (e.g., type of environment, salinity regimes, and cave zone as well as geographical information on the distribution of species in cave and anchialine environments. Biodiversity data will be progressively assembled from individual database sources at regional, national or local levels, as well as from literature sources (estimate: >20,000 existing records of cave-dwelling species scattered in several databases. Information will be organized in the WoRCS database following a standard glossary based on existing terminology. Cave-related information will be managed by the WoRCS thematic editors with all data dynamically linked to WoRMS and its team of taxonomic editors. In order to mobilize data into global biogeographic databases, a Gazetteer of the Marine and Anchialine Caves of the World will be established. The presence records of species could be eventually georeferenced for submission to the Ocean Biogeographic Information System (OBIS and constitute an important dataset for biogeographical and

  7. The effect of social environment on alternative mating tactics in male Endler’s guppy, Poecilia wingei

    Czech Academy of Sciences Publication Activity Database

    Řežucha, Radomil; Reichard, Martin

    2014-01-01

    Roč. 88, č. 2 (2014), s. 195-202 ISSN 0003-3472 R&D Projects: GA ČR GA206/09/1163 Institutional support: RVO:68081766 Keywords : alternative mating tactics * courtship * experience * Poecilia wingei * sneaking * social environment Subject RIV: EG - Zoology Impact factor: 3.137, year: 2014

  8. Quantitative assessment of hepatic function: modified look-locker inversion recovery (MOLLI) sequence for T1 mapping on Gd-EOB-DTPA-enhanced liver MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Jeong Hee [Seoul National University Hospital, Department of Radiology, Seoul (Korea, Republic of); Lee, Jeong Min; Han, Joon Koo; Choi, Byung Ihn [Seoul National University Hospital, Department of Radiology, Seoul (Korea, Republic of); Seoul National University College of Medicine, Institute of Radiation Medicine, Jongno-gu, Seoul (Korea, Republic of); Paek, Munyoung [Siemens Healthcare, Seoul (Korea, Republic of)

    2016-06-15

    To determine whether multislice T1 mapping of the liver using a modified look-locker inversion recovery (MOLLI) sequence on gadoxetic acid-enhanced magnetic resonance imaging (MRI) can be used as a quantitative tool to estimate liver function and predict the presence of oesophageal or gastric varices. Phantoms filled with gadoxetic acid were scanned three times using MOLLI sequence to test repeatability. Patients with chronic liver disease or liver cirrhosis who underwent gadoxetic acid-enhanced liver MRI including MOLLI sequence at 3 T were included (n = 343). Pre- and postcontrast T1 relaxation times of the liver (T1liver), changes between pre- and postcontrast T1liver (ΔT1liver), and adjusted postcontrast T1liver (postcontrast T1liver-T1spleen/T1spleen) were compared among Child-Pugh classes. In 62 patients who underwent endoscopy, all T1 parameters and spleen sizes were correlated with varices. Phantom study showed excellent repeatability of MOLLI sequence. As Child-Pugh scores increased, pre- and postcontrast T1liver were significantly prolonged (P < 0.001), and ΔT1liver and adjusted postcontrast T1liver decreased (P< 0.001). Adjusted postcontrast T1liver and spleen size were independently associated with varices (R{sup 2} = 0.29, P < 0.001). T1 mapping of the liver using MOLLI sequence on gadoxetic acid-enhanced MRI demonstrated potential in quantitatively estimating liver function, and adjusted postcontrast T1liver was significantly associated with varices. (orig.)

  9. An Investigation of Meromixis in Cave Pools, Lechuguilla Cave, New Mexico.

    Directory of Open Access Journals (Sweden)

    David B. Levy

    2008-07-01

    Full Text Available Chemical characteristics of permanent stratification in cave pools (meromixis may provide insight into the geochemical origin and evolution of cave pool waters. The objective of this study was to test the hypothesis that some pools in Lechuguilla Cave may be subject to ectogenic meromixis, where permanent chemical stratification is induced by input of relatively saline or fresh water from an external source. However, because organic C concentrations in Lechuguilla waters are low (typically 0.9 m, and are probably the result of localized and transient atmospheric CO2(g concentrations. At LOBG, an EC increase of 93 µS cm-1 at the 0.9-m depth suggests meromictic conditions which are ectogenic, possibly due to surface inflow of fresh water as drips or seepage into a pre-existing layer of higher salinity.

  10. Hydrological characterization of cave drip waters in a porous limestone: Golgotha Cave, Western Australia

    Science.gov (United States)

    Mahmud, Kashif; Mariethoz, Gregoire; Baker, Andy; Treble, Pauline C.

    2018-02-01

    Cave drip water response to surface meteorological conditions is complex due to the heterogeneity of water movement in the karst unsaturated zone. Previous studies have focused on the monitoring of fractured rock limestones that have little or no primary porosity. In this study, we aim to further understand infiltration water hydrology in the Tamala Limestone of SW Australia, which is Quaternary aeolianite with primary porosity. We build on our previous studies of the Golgotha Cave system and utilize the existing spatial survey of 29 automated cave drip loggers and a lidar-based flow classification scheme, conducted in the two main chambers of this cave. We find that a daily sampling frequency at our cave site optimizes the capture of drip variability with the least possible sampling artifacts. With the optimum sampling frequency, most of the drip sites show persistent autocorrelation for at least a month, typically much longer, indicating ample storage of water feeding all stalactites investigated. Drip discharge histograms are highly variable, showing sometimes multimodal distributions. Histogram skewness is shown to relate to the wetter-than-average 2013 hydrological year and modality is affected by seasonality. The hydrological classification scheme with respect to mean discharge and the flow variation can distinguish between groundwater flow types in limestones with primary porosity, and the technique could be used to characterize different karst flow paths when high-frequency automated drip logger data are available. We observe little difference in the coefficient of variation (COV) between flow classification types, probably reflecting the ample storage due to the dominance of primary porosity at this cave site. Moreover, we do not find any relationship between drip variability and discharge within similar flow type. Finally, a combination of multidimensional scaling (MDS) and clustering by k means is used to classify similar drip types based on time series

  11. Hydrological characterization of cave drip waters in a porous limestone: Golgotha Cave, Western Australia

    Directory of Open Access Journals (Sweden)

    K. Mahmud

    2018-02-01

    Full Text Available Cave drip water response to surface meteorological conditions is complex due to the heterogeneity of water movement in the karst unsaturated zone. Previous studies have focused on the monitoring of fractured rock limestones that have little or no primary porosity. In this study, we aim to further understand infiltration water hydrology in the Tamala Limestone of SW Australia, which is Quaternary aeolianite with primary porosity. We build on our previous studies of the Golgotha Cave system and utilize the existing spatial survey of 29 automated cave drip loggers and a lidar-based flow classification scheme, conducted in the two main chambers of this cave. We find that a daily sampling frequency at our cave site optimizes the capture of drip variability with the least possible sampling artifacts. With the optimum sampling frequency, most of the drip sites show persistent autocorrelation for at least a month, typically much longer, indicating ample storage of water feeding all stalactites investigated. Drip discharge histograms are highly variable, showing sometimes multimodal distributions. Histogram skewness is shown to relate to the wetter-than-average 2013 hydrological year and modality is affected by seasonality. The hydrological classification scheme with respect to mean discharge and the flow variation can distinguish between groundwater flow types in limestones with primary porosity, and the technique could be used to characterize different karst flow paths when high-frequency automated drip logger data are available. We observe little difference in the coefficient of variation (COV between flow classification types, probably reflecting the ample storage due to the dominance of primary porosity at this cave site. Moreover, we do not find any relationship between drip variability and discharge within similar flow type. Finally, a combination of multidimensional scaling (MDS and clustering by k means is used to classify similar drip

  12. Discovery of a diverse cave flora in China

    Science.gov (United States)

    Wen, Fang

    2018-01-01

    Few studies document plants in caves. Our field observations of a widespread and seemingly angiosperm-rich cave flora in SW China lead us to test the following hypotheses, 1) SW China caves contain a diverse vascular plant flora, 2) that this is a relic of a largely absent forest type lacking endemic species, and 3) that the light environment plants occupy in caves is not distinct from non-cave habitats. To do so we surveyed 61 caves and used species accumulation curves (SAC) to estimate the total diversity of this flora and used a subsample of 14 caves to characterise the light environment. We used regional floras and existing conservation assessments to evaluate the conservation value of this flora. We used observations on human disturbance within caves to evaluate anthropogenic activities. Four-hundred-and-eighteen vascular plant species were documented with SACs predicting a total diversity of 529–846. Ninety-three percent of the species documented are known karst forest species, 7% are endemic to caves and 81% of the species are angiosperms. We demonstrate that the light environment in caves is distinct to that of terrestrial habitats and that a subset of the flora likely grow in the lowest light levels documented for vascularised plants. Our results suggest that the proportion of species threatened with extinction is like that for the terrestrial habitat and that almost half of the entrance caverns sampled showed signs of human disturbance. We believe that this is the first time that such an extensive sample of cave flora has been undertaken and that such a diverse vascular plant flora has been observed in caves which we predict occurs elsewhere in SE Asia. We argue that the cave flora is an extension of the karst forest understory present prior to catastrophic deforestation in the 20thC. We suggest that within SW China caves serve as both refuges and a valuable source of germplasm for the restoration of karst forest. We also propose that caves represent a

  13. Discovery of a diverse cave flora in China.

    Science.gov (United States)

    Monro, Alexandre K; Bystriakova, Nadia; Fu, Longfei; Wen, Fang; Wei, Yigang

    2018-01-01

    Few studies document plants in caves. Our field observations of a widespread and seemingly angiosperm-rich cave flora in SW China lead us to test the following hypotheses, 1) SW China caves contain a diverse vascular plant flora, 2) that this is a relic of a largely absent forest type lacking endemic species, and 3) that the light environment plants occupy in caves is not distinct from non-cave habitats. To do so we surveyed 61 caves and used species accumulation curves (SAC) to estimate the total diversity of this flora and used a subsample of 14 caves to characterise the light environment. We used regional floras and existing conservation assessments to evaluate the conservation value of this flora. We used observations on human disturbance within caves to evaluate anthropogenic activities. Four-hundred-and-eighteen vascular plant species were documented with SACs predicting a total diversity of 529-846. Ninety-three percent of the species documented are known karst forest species, 7% are endemic to caves and 81% of the species are angiosperms. We demonstrate that the light environment in caves is distinct to that of terrestrial habitats and that a subset of the flora likely grow in the lowest light levels documented for vascularised plants. Our results suggest that the proportion of species threatened with extinction is like that for the terrestrial habitat and that almost half of the entrance caverns sampled showed signs of human disturbance. We believe that this is the first time that such an extensive sample of cave flora has been undertaken and that such a diverse vascular plant flora has been observed in caves which we predict occurs elsewhere in SE Asia. We argue that the cave flora is an extension of the karst forest understory present prior to catastrophic deforestation in the 20thC. We suggest that within SW China caves serve as both refuges and a valuable source of germplasm for the restoration of karst forest. We also propose that caves represent a

  14. Helium Isotopes and Noble Gas Abundances of Cave Dripping Water in Three Caves in East Asia

    Science.gov (United States)

    Chen, A. T.; Shen, C. C.; Tan, M.; Li, T.; Uemura, R.; Asami, R.

    2015-12-01

    Paleo-temperature recorded in nature archives is a critical parameter to understand climate change in the past. With advantages of unique inert chemical characteristics and sensitive solubilities with temperature, dissolved noble gases in speleothem inclusion water were recently proposed to retrieve terrestrial temperature history. In order to accurately apply this newly-developed speleothem noble gas temperature (NGT) as a reliable proxy, a fundamental issue about behaviors of noble gases in the karst should be first clarified. In this study, we measured noble gas contents in air and dripping water to evaluate any ratio deviation between noble gases. Cave dripping water samples was collected from three selected caves, Shihua Cave in northern China, Furong Cave in southwestern, and Gyukusen Cave in an island located in the western Pacific. For these caves are characterized by a thorough mixing and long-term storage of waters in a karst aquifer by the absence of seasonal oxygen isotope shifts. Ratios of dripping water noble gases are statistically insignificant from air data. Helium isotopic ratios in the dripping water samples match air value. The results indicate that elemental and isotopic signatures of noble gases from air can be frankly preserved in the epikarst and support the fidelity of NGT techniques.

  15. Radon survey in caves from Mallorca Island, Spain

    Energy Technology Data Exchange (ETDEWEB)

    Dumitru, Oana A. [Department of Geology, Babeș-Bolyai University, Kogălniceanu 1, 400084 Cluj-Napoca (Romania); School of Geosciences, University of South Florida, 4202 E. Fowler Ave., NES 107 Tampa (United States); Onac, Bogdan P. [School of Geosciences, University of South Florida, 4202 E. Fowler Ave., NES 107 Tampa (United States); Fornós, Joan J. [Departament de Ciències de la Terra, Universitat de les Illes Balears, Crta. Valldemossa km 7.5, 07122 Palma (Mallorca) (Spain); Cosma, Constantin [Environmental Radioactivity and Nuclear Dating Center, Babeș-Bolyai University, Fântânele 30, 400294 Cluj-Napoca (Romania); Ginés, Angel; Ginés, Joaquín [Departament de Ciències de la Terra, Universitat de les Illes Balears, Crta. Valldemossa km 7.5, 07122 Palma (Mallorca) (Spain); Merino, Antoni [Grup Espeleològic de Llubí, Federació Balear d' Espeleologia, c/Uruguai s/n, Palma Arena, 07010 Palma, Illes Balears (Spain)

    2015-09-01

    This study reports radon concentration in the most representative caves of Mallorca, identifying those in which the recommended action level is exceeded, thus posing health risks. Two show caves (Campanet and Artà) and three non-touristic caves (Font, Drac, Vallgornera) were investigated. Data were collected at several locations within each cave for three different periods, from March 2013 to March 2014. Except for Vallgornera, where only one monitoring period was possible, and Artà in which low values were recorded throughout the year, a clear seasonal variability, with higher values during the warm seasons and lower during winter time is prominent. Radon concentrations differed markedly from one cave to another, as well as within the same cave, ranging from below detection limit up to 3060 Bq·m{sup −3}. The results of this study have significant practical implications, making it possible to provide some recommendation to cave administrators and other agencies involved in granting access to the investigated caves. - Highlights: • A survey of radon was carried out in caves from Mallorca, Spain using CR 39 detectors. • Three different seasons are covered: spring, summer, and winter. • Radon level ranges from below detection limit up to 3060 Bq·m{sup −3}. • Seasonal variation is evident (higher values in summer and lower during winter). • Particular recommendations were made to each cave administration.

  16. The future of the CAVE

    KAUST Repository

    DeFanti, Thomas; Acevedo-Feliz, Daniel; Ainsworth, Richard; Brown, Maxine; Cutchin, Steven; Dawe, Gregory; Doerr, Kai-Uwe; Johnson, Andrew; Knox, Christopher; Kooima, Robert; Kuester, Falko; Leigh, Jason; Long, Lance; Otto, Peter; Petrovic, Vid; Ponto, Kevin; Prudhomme, Andrew; Rao, Ramesh; Renambot, Luc; Sandin, Daniel; Schulze, Jurgen; Smarr, Larry; Srinivasan, Madhusudhanan; Weber, Philip; Wickham, Gregory

    2011-01-01

    The CAVE, a walk-in virtual reality environment typically consisting of 4–6 3 m-by-3 m sides of a room made of rear-projected screens, was first conceived and built in 1991. In the nearly two decades since its conception, the supporting technology has improved so that current CAVEs are much brighter, at much higher resolution, and have dramatically improved graphics performance. However, rear-projection-based CAVEs typically must be housed in a 10 m-by-10 m-by-10 m room (allowing space behind the screen walls for the projectors), which limits their deployment to large spaces. The CAVE of the future will be made of tessellated panel displays, eliminating the projection distance, but the implementation of such displays is challenging. Early multi-tile, panel-based, virtual-reality displays have been designed, prototyped, and built for the King Abdullah University of Science and Technology (KAUST) in Saudi Arabia by researchers at the University of California, San Diego, and the University of Illinois at Chicago. New means of image generation and control are considered key contributions to the future viability of the CAVE as a virtual-reality device.

  17. Paleo-watertable definition using cave ferromanganese stromatolites and associated cave-wall notches (Sierra de Arnero, Spain)

    Science.gov (United States)

    Rossi, Carlos; Villalaín, Juan J.; Lozano, Rafael P.; Hellstrom, John

    2016-05-01

    The steeply-dipping-dolostone-hosted caves of the Sierra de Arnero (N Spain) contain low-gradient relict canyons with up to ten mapped levels of ferromanganese stromatolites and associated wall notches over a vertical range of 85 m, the highest occurring 460 m above base level. Despite a plausible speleogenetic contribution by pyrite oxidation, and the irregular cave-wall mesomorphologies suggestive of hypogenic speleogenesis, the Arnero relict caves are dominantly epigenic, as indicated by the conduit pattern and the abundant allogenic sediments. Allogenic input declined over time due to a piracy-related decrease in the drainage area of allogenic streams, explaining the large size of the relict Arnero caves relative to the limited present-day outcrop area of the karstified carbonates. Allogenic-sediment input also explains the observed change from watertable canyons to phreatic conduits in the paleo-downstream direction. Stromatolites and notches arguably formed in cave-stream passages at the watertable. The best-defined paleo-watertables show an overall slope of 1.7°, consistent with the present-day relief of the watertable, with higher-slope segments caused by barriers related to sulfide mineralization. The formation of watertable stromatolites favored wall notching by the combined effect of enhanced acidity by Mn-Fe oxidation and shielding of cave floors against erosion. Abrasive bedload further contributed to notch formation by promoting lateral mechanical erosion and protecting passage floors. The irregular wallrock erosional forms of Arnero caves are related partly to paragenesis and partly to the porous nature of the host dolostones, which favored irregular dissolution near passage walls, generating friable halos. Subsequent mechanical erosion contributed to generate spongework patterns. The dolostone porosity also contributes to explain the paradox that virtually all Arnero caves are developed in dolostone despite being less soluble than adjacent

  18. Book Review: Caves and Karst of the Yorkshire Dales

    Science.gov (United States)

    Westaway, Rob

    2015-10-01

    The British Cave Research Association (BCRA) is the research division of the British Caving Association (BCA), itself the principal society in Britain for those interested in caving, with activities including provision of training and safety certification for cavers and managing access to caves. Although some UK cave-related research is carried out by academics, this tends to be restricted to archaeological investigations of caves that have served as human habitations, and to be focused more on the occupants than the caves themselves. In contrast, most cave exploration is undertaken as a leisure activity, under the auspices of clubs affiliated to the BCA/BCRA, this being indeed virtually the only field of Earth science where amateur investigators can continue to make significant discoveries. Many cave explorers are also affiliated with academic researchers, such as managers of dating laboratories; the synergy between these two groups is highly productive, having resulted for instance in the discovery and exploration in recent years of the vast Ogof Draenen cave system in South Wales, which probably dates back to the Early Pleistocene (e.g., Farrant et al., 2014).

  19. Nonrandom variation in Poecilia marcellinoi n. sp. and P. salvatoris Regan, 1907 in El Salvador (Pisces, Poeciliidae)

    NARCIS (Netherlands)

    Poeser, Frederick N.

    1995-01-01

    A new species of the genus Poecilia Bloch & Schneider, 1801 is recorded from El Salvador. Morphological and meristic data of P. marcellinoi n. sp. and of the sympatric P. salvatoris Regan, 1907 are analysed. Intra- and interspecific variation are compared and correlated with environmental and

  20. PIXE analysis of cave sediments, prehispanic paintings and obsidian cutting tools from Baja California Sur caves

    International Nuclear Information System (INIS)

    Miranda, J.; Oliver, A.; Dacal, A.; Ruvalcaba, J.L.; Cruz, F.; Ortiz, M.E.; Vinas, R.

    1993-01-01

    Elemental PIXE analysis of cave sediments, minerals, pigments of the prehispanic paintings and obsidian cutting tools from caves in Baja California Sur has been carried out with a 0.7 MeV proton beam. The elements analysed in this sample set (Al to Co) provide an idea of the environment of the caves. The obsidian data analysis suggests that the human communities who made these painting used more than one obsidian source to manufacture cutting tools. (orig.)

  1. Exposure to radon in Australian tourist caves

    International Nuclear Information System (INIS)

    Solomon, S.B.; Langroo, R.; Lyons, R.G.

    1994-01-01

    In 1991 the International Commission on Radiological Protection(ICRP) produced guidelines and recommendations dealing with workplace exposure to elevated background radiation, in particular, the risk associated with the inhalation of radon and radon progeny. An intervention level of 1000 Bq m -3 has been proposed. Australia has over 40 tourist caves, under the management of the various State Departments or private groups. The limited data available on radon levels in Australian caves would suggest that some of these caves may be in excess of the proposed intervention level, thus presenting a potential health risk for the cave guides. This paper summarises the current information on radon in Australian caves and describes the proposed methodologies to be used for a Worksafe Australia-funded survey of radon levels in Tourist caves within Australia. This survey is to be carried out jointly by researchers at the Australian Radiation Laboratory, University of Auckland and the University of Sydney, during 1994 and 1995. 7 refs., 3 tabs

  2. ACTIVIDAD ANTIMICÓTICA DEL ACEITE ESENCIAL DE LAS HOJAS DE Minthostachys mollis (MUÑA COMPARADO CON EL FLUCONAZOL EN CULTIVO DE Candida albicans

    Directory of Open Access Journals (Sweden)

    Katherine M Alcalá-Marcos

    2011-01-01

    Full Text Available Objetivo.- Demostrar el efecto antimicótico del aceite esencial de las hojas de Minthostachys mollis (muña en comparación con el Fluconazol en cultivo de Candida albicans. Materiales y Método.- Estudio experimental. El efecto antimicótico se estudió midiendo 80 halos de inhibición distribuidos en 5 grupos mediante el método Kirby-Bauer. Se utilizó una cepa clínica de Candida albicans. Los grupos de estudio fueron grupo muña 25% (GM25%, grupo muña 50% (GM50%, grupo muña 100% (GM100%, un grupo control positivo (Fluconazol, y un grupo control negativo (aceite mineral. El análisis estadístico se realizó mediante la Prueba de Kruskal-Wallis y el Test de Dunn usando el paquete SPSS v.17.0. Se consideró un nivel de significancia 0,05.Conclusión.- El aceite esencial de las hojas de Minthostachys mollis (al 100% tuvo mayor efecto contra la Candida albicans que el Fluconazol; además, el efecto antimicótico del Fluconazol fue mayor que la Minthostachys mollis al 25%, y fue el mismo que la Minthostachys mollis al 50%.

  3. Minerogenetic mechanisms occurring in the cave environment: an overview

    Directory of Open Access Journals (Sweden)

    Forti Paolo

    2011-07-01

    Full Text Available Perhaps man’s first motivation to explore caves, beyond using them as shelter, was the search for substances that were not availableelsewhere: most of them were minerals. However, for a long time it was believed that the cave environment was not very interestingfrom the mineralogical point of view. This was due to the fact that most cave deposits are normally composed of a singlecompound: calcium carbonate. Therefore, the systematic study of cave mineralogy is of only recent origin. However, although onlya limited number of natural cavities have been investigated in detail, about 350 cave minerals have already been observed, someof which are new to science. The presence of such unexpected richness is a direct consequence of the variety of rocks traversedby water or other fluids before entering a cave and the sediments therein. Different cave environments allow the development ofvarious minerogenetic mechanisms, the most important of which are double exchange reactions, evaporation, oxidation, hydrationdehydration,sublimation, deposition from aerosols and vapors, and segregation. The cave temperature and pH/Eh strictly controlmost of them, although some are driven by microorganisms. The cave environment, due to its long-term stability, can sometimesallow for the development of huge euhedral crystals, such as those found in the Naica caves (Mexico, but the presence of extremelysmall yet complex aggregates of different minerals is far more common. Future development in the field of cave mineralogy will likelybe focused mainly on hydrothermal and sulfuric-acid caves and on the role played by micro-organisms in controlling some of the mostimportant minerogenetic processes in caves.

  4. Pathogenic and opportunistic microorganisms in caves

    Directory of Open Access Journals (Sweden)

    Sanchez-Moral Sergio

    2010-01-01

    Full Text Available With today’s leisure tourism, the frequency of visits to many caves makes it necessary to know about possible potentially pathogenic microorganisms in caves, determine their reservoirs, and inform the public about the consequences of such visits. Our data reveal that caves could be a potential danger to visitors because of the presence of opportunistic microorganisms, whose existence and possible development in humans is currently unknown.

  5. Diurnal Variation of Radon Concentration in the Postojna Cave

    International Nuclear Information System (INIS)

    Gregoric, A.; Vaupotic, J.

    2011-01-01

    Postojna Cave, with 20 km of galleries, is the longest known cave system and also the largest of about 20 show caves in Slovenia and one of the most visited show caves in the world. It is well known that high concentrations of radon are common in karstic caves, although quantities of uranium (238U) in limestone are rather low. The reason for this is low natural ventilation of the underground cavities. Tectonic faults constitute an additional source of radon. Variations of radon concentration in cave air arise from a balance of the emission from cave surfaces and drip waters, decay in cave air, and exchange with the outside atmosphere. Because of its elevated radon concentrations, Postojna Cave has been under permanent radon survey since 1995. The influence of meteorological conditions on the radon levels and their temporal variations depends mostly on the shape of the cave, and the number and directions of cracks, corridors and fissures connecting the cave rooms with the outside atmosphere. The driving force for air movement in horizontal caves, and thus the inflow of fresh air and release of the cave air to the atmosphere, is the temperature difference between the cave air and outdoors, which causes seasonal pattern of radon concentration in the cave with high levels in summer and low in winter. However, on a daily scale different behaviour of radon can be observed at different locations in the cave. In this paper diurnal variation of radon concentration at two locations is presented and discussed. Postojna Cave is a horizontal cave with a stable yearly temperature around 10 degrees of @C. Continuous measurements of radon concentration were carried out from 2005 to 2010 at two locations along the guided tourist trail. Radon concentration was measured with Radim 5 WP monitors (SMM Company, Prague, Czech Republic) with sampling frequency once an hour. The evaluation of five-year radon monitoring at two sites in the Postojna Cave reveals significant diurnal and

  6. Microbiological and environmental issues in show caves.

    Science.gov (United States)

    Saiz-Jimenez, Cesareo

    2012-07-01

    Cultural tourism expanded in the last half of the twentieth century, and the interest of visitors has come to include caves containing archaeological remains. Some show caves attracted mass tourism, and economical interests prevailed over conservation, which led to a deterioration of the subterranean environment and the rock art. The presence and the role of microorganisms in caves is a topic that is often ignored in cave management. Knowledge of the colonisation patterns, the dispersion mechanisms, and the effect on human health and, when present, over rock art paintings of these microorganisms is of the utmost importance. In this review the most recent advances in the study of microorganisms in caves are presented, together with the environmental implications of the findings.

  7. Genomic sequencing of Pleistocene cave bears

    Energy Technology Data Exchange (ETDEWEB)

    Noonan, James P.; Hofreiter, Michael; Smith, Doug; Priest, JamesR.; Rohland, Nadin; Rabeder, Gernot; Krause, Johannes; Detter, J. Chris; Paabo, Svante; Rubin, Edward M.

    2005-04-01

    Despite the information content of genomic DNA, ancient DNA studies to date have largely been limited to amplification of mitochondrial DNA due to technical hurdles such as contamination and degradation of ancient DNAs. In this study, we describe two metagenomic libraries constructed using unamplified DNA extracted from the bones of two 40,000-year-old extinct cave bears. Analysis of {approx}1 Mb of sequence from each library showed that, despite significant microbial contamination, 5.8 percent and 1.1 percent of clones in the libraries contain cave bear inserts, yielding 26,861 bp of cave bear genome sequence. Alignment of this sequence to the dog genome, the closest sequenced genome to cave bear in terms of evolutionary distance, revealed roughly the expected ratio of cave bear exons, repeats and conserved noncoding sequences. Only 0.04 percent of all clones sequenced were derived from contamination with modern human DNA. Comparison of cave bear with orthologous sequences from several modern bear species revealed the evolutionary relationship of these lineages. Using the metagenomic approach described here, we have recovered substantial quantities of mammalian genomic sequence more than twice as old as any previously reported, establishing the feasibility of ancient DNA genomic sequencing programs.

  8. Distinction between epigenic and hypogenic maze caves

    Science.gov (United States)

    Palmer, Arthur N.

    2011-11-01

    Certain caves formed by dissolution of bedrock have maze patterns composed of closed loops in which many intersecting fractures or pores have enlarged simultaneously. Their origin can be epigenic (by shallow circulation of meteoric groundwater) or hypogenic (by rising groundwater or production of deep-seated solutional aggressiveness). Epigenic mazes form by diffuse infiltration through a permeable insoluble caprock or by floodwater supplied by sinking streams. Most hypogenic caves involve deep sources of aggressiveness. Transverse hypogenic cave origin is a recently proposed concept in which groundwater of mainly meteoric origin rises across strata in the distal portions of large flow systems, to form mazes in soluble rock sandwiched between permeable but insoluble strata. The distinction between maze types is debated and is usually based on examination of diagnostic cave features and relation of caves to their regional setting. In this paper, the principles of mass transfer are applied to clarify the limits of each model, to show how cave origin is related to groundwater discharge, dissolution rate, and time. The results show that diffuse infiltration and floodwater can each form maze caves at geologically feasible rates (typically within 500 ka). Transverse hypogenic mazes in limestone, to enlarge significantly within 1 Ma, require an unusually high permeability of the non-carbonate beds (generally ≥ 10-4 cm/s), large discharge, and calcite saturation no greater than 90%, which is rare in deep diffuse flow in sedimentary rocks. Deep sources of aggressiveness are usually required. The origin of caves by transverse hypogenic flow is much more favorable in evaporite rocks than in carbonate rocks.

  9. USE OF THE JAPANESE MEDAKA (ORYZIAS LATIPES) AND GUPPY (POECILIA RETICULATA) IN CARCINOGENESIS TESTING UNDER NATIONAL TOXICOLOGY PROGRAM PROTOCOLS

    Science.gov (United States)

    that are economical, sensitive, and scientifically acceptable. Among small fish models, the Japanese medaka (Oryzias latipes) is preeminent for investigating effects of carcinogenic and/or toxic waterborne hazards to humans. The guppy (Poecilia reticulata), although less widely u...

  10. Structural and hydrological controls on the development of a river cave in marble (Tapagem Cave - SE Brazil

    Directory of Open Access Journals (Sweden)

    William Sallun Filho

    2015-01-01

    Full Text Available Tapagem Cave (or Devil’s Cave is a river cave developed in the dolomite marble karst of the Serra do André Lopes (State of São Paulo, southeastern Brazil. Although this region is a plateau with significant variation in elevation and a humid subtropical climate, the cave is an anomalous feature in the André Lopes karst because there are few other caves. The marble, which is in a synclinal structure with subjacent phyllites, is a karst aquifer perched above the regional base level (Ribeira River and has little allogenic recharge. The cave developed on a secondary anticline on the northwest flank of the marble synform forming a blind valley, the Tapagem River sink, that is an underground tributary of Ostras River. Development of the cave is due to the entrenchment of the Ostras through-valley and the large allogenic catchment area of the sink. In plan view, the morphology of the cave can be divided into three different sectors. The first sector, known as the Tourist Sector, has extensive collapse rooms, fossil passages and a variety of speleothems of notable dimensions. The second and most extensive sector is the river passage, which is a sinuous gallery controlled by marble banding with NE-SW cleavage and NW-SE fractures. In cross-section, the passages are vadose canyons up to 70 m in height, controlled by the marble banding. Four NW-SE diabase dykes in this passage do not affect its direction in plan view. The third sector is an extensive network of passages and collapse rooms, which are interlaced in plan view and on different levels, forming a maze pattern. Initially, the Tapagem and Ostras Rivers developed on a gentle surface and flowed into the Ribeira River. With the entrenchment of the Ostras through-valley, the Tapagem River partially infiltrated via a paleosink into the upper passage of the “Erectus Room," remaining a half-blind valley. Following a series of collapses and obstructions, the River next infiltrated via the current

  11. Urang Cave Karst Environmental Development, as Tourism Object

    Directory of Open Access Journals (Sweden)

    Srijono Srijono

    2013-12-01

    Full Text Available Karst environment become an alternative tourist destination as well as to boost local revenues. In karst environments in Grobogan District, Central Java Province, formed Urang Cave, with an interesting endokarst phenomenon. This study aims to do zoning district Urang Cave as tourist sites. The research method is using contour maps as a base map of Urang Cave karst environment geomorphological mapping. Geomorphological data processing is using ArcView GIS 3.3 program. Land use map refers to RBI, scale 1:25.000. Geomorphological analysis refers to the Ministry of Energy and Mineral Resources No. 1456.K/20/MEM/2000, and petrography. Each development zone is analyzed its geophysical environmental element, then set scoring and value summation. For comprehensive environmental element analysis, chemical analysis of rocks, and water-soil chemistry. In reference to Minister of Energy Mineral Resource decrees No. 1456/K/20/MEM/2000, Urang Cave zoning defined into 3 (three zone, as follow: the Protected Zone, Cultivation Zone 1, and Cultivation Zone 2. Protected Zone, consists of Urang Cave tunnel/hallway with a unique spheleothem in it. This zone as a cave tracking site tourism, potential to produce karst water as a decent drinking water while maintaining hardness. Cultivation Zone 1 is spreading about 200 m in distance from outer appearance of spring around the cave hallway. In this zone mining of cave sediments may be done in the inactive form caves, without changing the state of the existing major exokarst morphology. Cultivation Zone 2, an outer zone, located farthest from the tunnel/hallway Urang Cave. Utilization of this zone as a limestone mining quarry, although only on a small scale.

  12. Measurements of radon concentrations at caves in Jeju

    Energy Technology Data Exchange (ETDEWEB)

    Go, S. H.; Kang, D. H.; Jung, B. J. [Cheju National University, Cheju (Korea, Republic of)

    2004-07-01

    Radon is a radioactive gas emitting {alpha} particles. It is chemically stable due to its inert characteristic. While its daughter products, {sup 218}Po, {sup 214}Bi, {sup 214}Pb and {sup 214}Po, attached with aerosol particles, is known to cause lung cancer. As radon is produced from uranium and thorium, it accumulates in poorly ventilative underground voids such as caves and mine. Radon concentrations at caves in Jeju were measured in this study. The measurements were made by setting three CR-39 detectors for 70 days at 2 {approx} 4 positions in Manjang, Hyupjae and Ssangyong caves. The radon levels of the caves spread 403.1 . 606.7 Bq/m{sup 3}. With these results, it is concluded that the Jeju caves have 6 times higher radon concentrations than ordinary house of 65.3 Bq/m{sup 3} and that they are higher than Seoul subway stations due to poor ventilation. While, the caves in Jeju have lower radon concentrations than limestone caves of Robin Hood. The radon concentration in the middle of Manjang cave is slightly higher than the action level in the work place of 500 Bq/m{sup 3} suggested by the ICRP. The measurement errors are estimated to be less than 5 % from its calibration factor.

  13. Measurements of radon concentrations at caves in Jeju

    International Nuclear Information System (INIS)

    Go, S. H.; Kang, D. H.; Jung, B. J.

    2004-01-01

    Radon is a radioactive gas emitting α particles. It is chemically stable due to its inert characteristic. While its daughter products, 218 Po, 214 Bi, 214 Pb and 214 Po, attached with aerosol particles, is known to cause lung cancer. As radon is produced from uranium and thorium, it accumulates in poorly ventilative underground voids such as caves and mine. Radon concentrations at caves in Jeju were measured in this study. The measurements were made by setting three CR-39 detectors for 70 days at 2 ∼ 4 positions in Manjang, Hyupjae and Ssangyong caves. The radon levels of the caves spread 403.1 . 606.7 Bq/m 3 . With these results, it is concluded that the Jeju caves have 6 times higher radon concentrations than ordinary house of 65.3 Bq/m 3 and that they are higher than Seoul subway stations due to poor ventilation. While, the caves in Jeju have lower radon concentrations than limestone caves of Robin Hood. The radon concentration in the middle of Manjang cave is slightly higher than the action level in the work place of 500 Bq/m 3 suggested by the ICRP. The measurement errors are estimated to be less than 5 % from its calibration factor

  14. Hypogene caves of the central Appalachian Shenandoah Valley in Virginia

    Science.gov (United States)

    Doctor, Daniel H.; Orndorff, Wil

    2017-01-01

    Several caves in the Shenandoah Valley in Virginia show evidence for early hypogenic conduit development with later-enhanced solution under partly confined phreatic conditions guided by geologic structures. Many (but not all) of these caves have been subsequently invaded by surface waters as a result of erosion and exhumation. Those not so affected are relict phreatic caves, bearing no relation to modern drainage patterns. Field and petrographic evidence shows that carbonate rocks hosting certain relict phreatic caves were dolomitized and/or silicified by early hydrothermal fluid migration in zones that served to locally enhance rock porosity, thus providing preferential pathways for later solution by groundwater flow, and making the surrounding bedrock more resistant to surficial weathering to result in caves that reside within isolated hills on the land surface. Features suggesting that deep phreatic processes dominated the development of these relict caves include (1) cave passage morphologies indicative of ascending fluids, (2) cave plans of irregular pattern, reflecting early maze or anastomosing development, (3) a general lack of cave breakdown and cave streams or cave stream deposits, and (4) calcite wall and pool coatings within isolated caves intersecting the local water table, and within unroofed caves at topographic locations elevated well above the local base level. Episodes of deep karstification were likely separated by long periods of geologic time, encompassing multiple phases of sedimentary fill and excavation within caves, and reflect a complex history of deep fluid migration that set the stage for later shallow speleogenesis that continues today.

  15. Bioaccumulation of eight heavy metals in cave animals from Dashui ...

    African Journals Online (AJOL)

    ajl2

    karst caves and water systems in the caves are well developed. So, heavy metals can contaminate cave envi- ronment and affect cave animals. Karst topography is widely distributed in Guizhou province, China, accounting for 73.8% of the total land area. So, the examination of heavy metal pollution in cave soil and water ...

  16. Phytochemical and antifungal activity of anthraquinones and root and leaf extracts of Coccoloba mollis on phytopathogens

    Directory of Open Access Journals (Sweden)

    Iuri Bezerra de Barros

    2011-06-01

    Full Text Available The aim of this work was to study the phytochemical and antifungal activity of anthraquinones and root and leaf extracts of Coccoloba mollis on phytopathogens. The chemical analysis of ethanolic extracts showed a mixture of long-chain hydrocarbons, carboxyl esters and 3-taraxerone in the leaf extract. Two anthraquinones (emodin and physcion were isolated and identified from the root extract. Phytochemical screening using the pharmacognostic methods revealed the presence of flavonoids and tannins in the leaves and roots. Anthraquinones were only found in the root extract, no alkaloids, coumarins, saponins and simple phenolics were present. The antifungal activity of C. mollis extracts and anthraquinones isolated from the root of this plant against Botryospheria ribis, B. rhodina, Lasiodiplodia theobromae and Fusarium sp showed promising results for their use as fungicides, where emodin was the most active compound, which inhibited the microorganisms tested up to 44%.

  17. Chemical composition and in vitro antibacterial activity of the essential oil of Minthostachys mollis (Kunth) Griseb Vaught from the Venezuelan Andes.

    Science.gov (United States)

    Mora, Flor D; Araque, María; Rojas, Luis B; Ramirez, Rosslyn; Silva, Bladimiro; Usubillaga, Alfredo

    2009-07-01

    Chemical constituents of the essential oil from the leaves of Minthostachys mollis (Kunth) Griseb Vaught var. mollis collected in January 2008 at Tuñame, Trujillo State, Venezuela, were separated and identified by GC-MS analysis. The essential oil was obtained by hydrodistillation and thirteen components (98.5% of the sample) were identified by comparison with the Wiley GC-MS library data base. The two major components were pulegone (55.2%) and trans-menthone (31.5%). The essential oil showed a significant inhibitory effect against Gram-positive and Gram-negative bacteria, especially Bacillus subtilis and Salmonella typhi (4 microg/mL).

  18. Measurements of radon levels inside Mexican caves

    International Nuclear Information System (INIS)

    Borau, J.; Gonzalez, A.; Espinosa, G.; Golzarri, J.I.

    1993-01-01

    Living animal species on earth have been exposed to environmental radon from the very beginning of time. The effects of radiation, combined with other natural parameters such as temperature, humidity, salt contents, etc., have most likely influenced the evaluation of different species. Thus, it is important to know and to evaluate the radon levels, among other radioactive elements present in enclosed environments such as caves, especially since those caves were also the dwellings and refuge of the predecessor of man. In this work we present radon level measurements inside some caves with vestiges of ancient inhabitats and some recently discovered natural caves, using Nuclear Track Detectors. (author)

  19. Contextualizing Cave Maps as Geospatial Information: Case Study of Indonesia

    Science.gov (United States)

    Reinhart, H.

    2017-12-01

    Caves are the result of solution processes. Because they are happened from geochemical and tectonic activity, they can be considered as geosphere phenomena. As one of the geosphere phenomena, especially at karst landform, caves have spatial dimensions and aspects. Cave’s utilizations and developments are increasing in many sectors such as hydrology, earth science, and tourism industry. However, spatial aspects of caves are poorly concerned dues to the lack of recognition toward cave maps. Many stakeholders have not known significances and importance of cave maps in determining development of a cave. Less information can be considered as the cause. Therefore, it is strongly necessary to put cave maps into the right context in order to make stakeholders realize the significance of it. Also, cave maps will be officially regarded as tools related to policy, development, and conservation act of caves hence they will have regulation in the usages and applications. This paper aims to make the contextualization of cave maps toward legal act. The act which is used is Act Number 4 Year 2011 About Geospatial Information. The contextualization is done by scrutinizing every articles and clauses related to cave maps and seek the contextual elements from both of them. The results are that cave maps can be regarded as geospatial information and classified as thematic geospatial information. The usages of them can be regulated through the Act Number 4 Year 2011. The regulations comprised by data acquisition, database, authorities, surveyor, and the obligation of providing cave maps in planning cave’s development and the environment surrounding.

  20. Millipedes (Diplopoda of twelve caves in Western Mecsek, Southwest Hungary

    Directory of Open Access Journals (Sweden)

    Angyal, D.

    2013-11-01

    Full Text Available Twelve caves of Western Mecsek, Southwest Hungary were examined between September 2010 and April 2013from the millipede (Diplopoda faunistical point of view. Ten species were found in eight caves, which consistedeutroglophile and troglobiont elements as well. The cave with the most diverse fauna was the Törökpince Sinkhole, while thetwo previously also investigated caves, the Abaligeti Cave and the Mánfai-kőlyuk Cave provided less species, which couldbe related to their advanced touristic and industrial utilization.

  1. Actividad antimicótica del aceite esencial de las hojas de Minthostachys mollis (muña comparado con el Fluconazol en cultivo de Candida albicans

    Directory of Open Access Journals (Sweden)

    Katherine M Alcalá-Marcos

    2012-09-01

    Full Text Available Objetivo.- Demostrar el efecto antimicótico del aceite esencial de las hojas de Minthostachys mollis (muña en comparación con el Fluconazol en cultivo de Candida albicans. Materiales y Método.- Estudio experimental. El efecto antimicótico se estudió midiendo 80 halos de inhibición distribuidos en 5 grupos mediante el método Kirby-Bauer. Se utilizó una cepa clínica de Candida albicans. Los grupos de estudio fueron grupo muña 25% (GM25%, grupo muña 50% (GM50%, grupo muña 100% (GM100%, un grupo control positivo (Fluconazol, y un grupo control negativo (aceite mineral. El análisis estadístico se realizó mediante la Prueba de Kruskal-Wallis y el Test de Dunn usando el paquete SPSS v.17.0. Se consideró un nivel de significancia  0,05.Conclusión.- El aceite esencial de las hojas de Minthostachys mollis (al 100% tuvo mayor efecto contra la Candida albicans que el Fluconazol; además, el efecto antimicótico del Fluconazol fue mayor que la Minthostachys mollis al 25%, y fue el mismo que la Minthostachys mollis al 50%.

  2. Caves as observatories for atmospheric thermal tides: an example from Ascunsă Cave, Romania

    Directory of Open Access Journals (Sweden)

    Virgil Drăgușin

    2018-01-01

    Full Text Available As part of a microclimate study at Ascunsă Cave, Romania, we used Gemini Tinytag Plus 2 data loggers to record cave air temperature variability. At one of the monitoring points we recognized the presence of semidiurnal cycles on the order of a few thousands of a degree Celsius that could be produced under the influence of the semidiurnal tidal components of the Sun (S2 or the Moon (M2. Using a Gemini Tinytag Plus 2 data logger with an external probe we measured core rock temperature and showed that it does not influence the cave air temperature on such short time scales. We thus rejected the possibility that Earth tides, mostly produced by the lunar tidal influence on the Earth’s crust, would have had a semidiurnal influence on cave air temperature. Moreover, time series analysis revealed a 12.00-hour periodicity in temperature data, specific for the S2, allowing us to assign these variations to the influence of the thermo-tidal action of the Sun. Using the Ideal Gas Law and assuming a constant volume and amount of air, we calculated that a theoretical change in atmospheric pressure of around 40 Pa was needed to produce the temperature changes we observed. This agrees with published values of atmospheric pressure changes induced by the semidiurnal solar component of the thermal tides (S2(t. We thus can assign the observed temperature changes to semidiurnal atmospheric pressure changes (S2(p induced by the thermal excitation of the Sun. Our study signals the possibility that readily available data from cave monitoring studies around the world could be used in the study of atmospheric tides. Moreover, it appears that Ascunsă Cave acts as a natural meteorological filter on a short time scale, removing the direct thermal influences of the Sun (especially night and day differences and preserving only the barometric information from the surface.

  3. Stress analysis of longwall top coal caving

    Energy Technology Data Exchange (ETDEWEB)

    Alehossein, H.; Poulsen, B.A. [CSIRO Exploration & Mining, Brisbane, Qld. (Australia)

    2010-01-15

    Longwall top coal caving (LTCC) is a relatively new method of mining thick coal seams that is currently achieving high productivity and efficiency. The technique is similar to traditional longwall mining in that a cutting head slices coal from the lower section of the coal seam onto a conveyor belt installed in front of the hydraulic support near the cutting face. In modern LTCC an additional rear conveyor belt is located behind the support, to which the flow of the caved coal from the upper part of the seam can be controlled by a moveable flipper attached to the canopy of the support. The mining method relies on the fracturing of the top coal by the front abutment pressure to achieve satisfactory caving into the rear conveyor. This paper develops a yield and caveability criterion based on in situ conditions in the top coal in advance of the mining face (yield) and behind the supports (caveability). Yielding and caving effects are combined into one single number called caving number (CN), which is the multiplication result of caving factor (CF) and yield factor (YF). Analytical derivations are based on in situ stress conditions, Mohr-Coulomb and/or Hoek-Brown rock failure criteria and an on-associated elastoplastic strain softening material behaviour. The yield and caveability criteria are in agreement with results from both numerical studies and mine data. The caving number is normalised to mining conditions of a reference Chinese mine (LMX mine) and is used to assess LTCC performance at fourteen other Chinese working longwalls that have had varying success with the LTCC technology. As a predictive model, results of this analytical/numerical study are useful to assess the potential success of caving in new LTCC operations and in different mining conditions.

  4. Radon Exposures in the Caves of Tenerife (Canary Islands)

    International Nuclear Information System (INIS)

    Pinza-Molina, C.; Alcaide, J.M.; Rodriques-Bethencourt, R.; Hernandez-Armas, J.

    1999-01-01

    There are more than a hundred volcanic caves and pits of various lengths on the island of Tenerife (Canary Islands, Spain). The results are presented of atmospheric radon measurements performed in three of these caves and in three pits. One of the caves selected, El Viento Cave, is nearly 20 km in length and is the longest volcanic tube in the Canarian archipelago and the second longest in the world. The measurements were performed over two distinct periods during the year using passive polycarbonate detectors. The mean radon concentrations range between 0.3 and 8 kBq.m -3 , the maximum value corresponds to a site located at 1850 m from the mouth of El Viento Cave. Possible touristic development of these caves has been taken into account in estimating the effective doses for visitors and guides (considered separately). The values obtained range from 0.3 to 100 μSv per visit for visitors. The largest effective dose would correspond to that for guides at 41 mSv.y -1 in the Viento Cave. This result would make protection against radiological hazards obligatory if the cave were to be developed as a site for tourism. (author)

  5. Eleven bones: More fossil remains of Cave Lions and Cave Hyaenas from the North Sea area

    NARCIS (Netherlands)

    Bosscha Erdbrink, D.P.

    1983-01-01

    Six fossil Cave Lion bones and five fossil Cave Hyaena bones are described. One lion bone and one hyaena bone were dredged from the Westerschelde ( = Western Scheldt, southwestern part of the Netherlands). The other specimens were recovered from the bottom of the North Sea, in the area West and

  6. Effect of Temperature on Reproduction and Sex Ratio of Guppy (Poecilia reticulata Peters)

    OpenAIRE

    H. Arfah; S. Mariam; . Alimuddin

    2007-01-01

    Water temperature could affect the reproduction of broodstock and sex ratio of progeny.  In this study, broodstock of guppy (Poecilia reticulata Peters) was reared in different temperature to determine its effect on reproduction of broodstock and sex ratio of their progeny. The result of study show that broodstock reared at 27°C produced more fry (16 males mean) than that of 30°C (10 males), while broodstock reared at 33°C produced no progeny.  Percentage of male fish produced by broodstock r...

  7. Radon in an underground cave system in Victoria

    International Nuclear Information System (INIS)

    Hedt, J.C.; Boal, T.J.

    1998-01-01

    Radon levels in a cave system in Victoria have been measured. The variation of radon and radon progeny levels with time, position throughout the cave and season have been determined. The radiation exposure of tour guides were estimated. The data is being used to develop a radiation management plan for the tour guides. Radon concentration within a cave system was proven to be dependent to a large extent upon the rate of air exchange with outside. Cave ventilation is the single most important factor in determining if there is diurnal variation in the radon concentration

  8. 14C AMS dating Yongcheon cave

    International Nuclear Information System (INIS)

    Lee, J.H.; Choe, K.; Kim, J.C.; Choi, S.H.; Kang, J.; Song, S.; Song, Y.M.; Jang, J.G.

    2013-01-01

    The biggest island in South Korea is Jeju Island, which lies 80 km south of the mainland and has one shield volcano, Mt. Halla. The volcanic island and its lava tubes were added to the world heritage list by UNESCO in 2007. Among the many lava tubes on the island, a unique cave had been accidentally found in 2005 while some workers were replacing a telephone pole. Until the discovery, it had been completely isolated from the outside by naturally-built sand blocks. Yongcheon cave is a lime-decorated lava tube showing both the properties of a volcanic lava tube and a limestone cave. This cave, about 3 km in length, is acknowledged to be the best of this type in the world and includes a large clean-water lake, lava falls, and richly developed speleothems inside it. Even though there is archaeological evidence from well preserved pottery that ancient people entered this place, the preservation of artifacts was ensured by a geological change that made later entrance difficult. We have collected charcoal samples scattered around the cave and dated them using AMS. Ages were in the range of ca. 1570-1260 BP (A.D. 340–880) and this corresponds to the Ancient Three Kingdoms and the Unified Silla era in Korean history. The 14 C AMS measurement results presented in this paper on wood charcoal provide precise dates which will be very useful not only to clarify the nature of human activities in this cave but also to provide reference dates when comparing other dating methods.

  9. Eogenetic caves in conglomerate: an example from Udin Boršt, Slovenia

    Directory of Open Access Journals (Sweden)

    Lipar Matej

    2011-01-01

    Full Text Available Udin Boršt is a karstified terrace of carbonate rock, which is of fluvioglacial origin, and is situated in the north-western part of Slovenia. There are 15 registered caves, which have been interpreted as caves in conglomerate, while karst of Udin Boršt itself was interpreted as conglomerate karst, shallow karst or isolated karst. In this article, caves in Udin Boršt have been interpreted as eogenetic caves. Based on porosity and bedding material, different types of caves and cave passages have developed. Four general types of eogenetic caves found in Udin Boršt are; linear stream caves, shelter caves, breakdown caves and vadose shafts.

  10. Verificação da atividade antibacteriana de sabonete líquido contendo extrato glicólico de Dimorphandra mollis Benth.

    Directory of Open Access Journals (Sweden)

    KETYLIN FERNANDA MIGLIATO

    2009-12-01

    Full Text Available Dimorphandra mollis Benth., Compositae, falsobarbatimão, é utilizada topicamente como cicatrizante, adstringente e antimicrobiano. No presente estudo, verificou-se a atividade antibacteriana de sabonete líquido contendo extrato glicólico de D. mollis (EGD em diferentes concentrações (8, 15 e 20% e em diferentes pHs (6 e 8. Foram preparadas cinco formulações (F de sabonete: F1 - triclosan (0,1%, F2 - EGD (8%, F3 - EGD (15%, F4 - EGD (20% e F5 - sem conservante. Cascas de D. mollis foram secas em estufa de ar circulante e pulverizadas. Os extratos brutos foram preparados por turbo-extração utilizando-se etanol. Após filtração, os extratos foram concentrados em evaporador rotatório, liofilizados e ressuspendidos em propilenoglicol para a obtenção do extrato glicólico. A atividade antibacteriana foi verificada pelo método de difusão em ágar, empregando cilindros em placa. Placas contendo Staphylococcus aureus, Pseudomonas aeruginosa e Escherichia coli foram incubadas a 37ºC durante 24 horas. Após incubação, as leituras foram realizadas com paquímetro, observando-se o diâmetro do halo de inibição de crescimento bacteriano. Verificouse que o sabonete líquido contendo triclosan provocou inibição do crescimento bacteriano em ambos os pHs; já os sabonetes sem conservante e contendo EGD, independente da concentração e do pH empregados, não apresentaram atividade antibacteriana. Palavras-chave: Atividade antibacteriana. Sabonete líquido. Dimorphandra mollis Benth.

  11. Millipedes (Diplopoda) from caves of Portugal

    DEFF Research Database (Denmark)

    P. S. Reboleira, Ana Sofia; Enghoff, Henrik

    2014-01-01

    in caves of the mainland and the island of Madeira has provided new data about the distribution and diversity of millipedes. A review of millipedes from caves of Portugal is presented, listing fourteen species belonging to eight families, among which six species are considered troglobionts...

  12. Feasibility Study of Padarangin Cave Slogohimo Wonogiri for Eco-Tourism

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    Kuswaji Dwi Priyono

    2004-01-01

    Full Text Available Area of Karst of mount Sewu in countryside of Padarangin own immeasurable of potency to be developed by besides activity of mining namely the tourism activity. Cave of nature found enable to can be developed as tourism, but date not yet been known how big potency of relevant tourism with the cave.  In line with the problems, this research aim to provide basic data of potency of cave and assess elegibility of cave. Padarangin for ecotourism in Wonogiri Regeny. Method used by survey of field and interview resident. Result of research indicate that cave of Padarangin have mount of cave at height 848 m msl which relative narrow, chamber horizontal as long as 63,8 m and vertical in 32,5 m. Needed by a special equipment to enter cave with capasities 10 – 15 people once the visit/incoming, owning value of sakral trusted by local society. The cave competent to be developed as tourism object of special enthusasm with visit limited.

  13. The fungal colonisation of rock-art caves: experimental evidence.

    Science.gov (United States)

    Jurado, Valme; Fernandez-Cortes, Angel; Cuezva, Soledad; Laiz, Leonila; Cañaveras, Juan Carlos; Sanchez-Moral, Sergio; Saiz-Jimenez, Cesareo

    2009-09-01

    The conservation of rock-art paintings in European caves is a matter of increasing interest. This derives from the bacterial colonisation of Altamira Cave, Spain and the recent fungal outbreak of Lascaux Cave, France-both included in the UNESCO World Heritage List. Here, we show direct evidence of a fungal colonisation of rock tablets in a testing system exposed in Altamira Cave. After 2 months, the tablets, previously sterilised, were heavily colonised by fungi and bacteria. Most fungi isolated were labelled as entomopathogens, while the bacteria were those regularly identified in the cave. Rock colonisation was probably promoted by the dissolved organic carbon supplied with the dripping and condensation waters and favoured by the displacement of aerosols towards the interior of the cave, which contributed to the dissemination of microorganisms. The role of arthropods in the dispersal of spores may also help in understanding fungal colonisation. This study evidences the fragility of rock-art caves and demonstrates that microorganisms can easily colonise bare rocks and materials introduced into the cavity.

  14. Dating the Lascaux cave gour formation

    International Nuclear Information System (INIS)

    Genty, D.; Touma, M.; Konik, S.; Konik, S.; Valladas, H.; Hellstrom, J.; Moreau, C.; Dumoulin, J.P.; Nouet, J.; Dauphin, Y.; Weil, R.

    2011-01-01

    Lascaux Cave is renowned for its outstanding prehistoric paintings, strikingly well-preserved over about 18,000 yr. While stalagmites and stalactites are almost absent in the cave, there is an extensive calcite flow-stone that covered a large part of the cave until its opening for tourists during the 1950's. The deposit comprises a succession of calcite rims, or 'gours', which allowed seepage water to pond in large areas in the cave. Their possible role in preservation of the cave paintings has often been evoked, but until now this deposit has not been studied in detail. Here, we present 24 new radiocarbon accelerator mass spectrometry (AMS) and 6 uranium-thorium (U-Th) analyses from the calcite of the gours, 4 AMS 14 C dates from charcoals trapped in the calcite, and 4 AMS 14 C analyses on organic matter extracted from the calcite. Combining the calibrated 14 C ages obtained on charcoals and organic matter and U-Th ages from 14 C analyses made on the carbonate, has allowed the calculation of the dead carbon proportion (dcp) of the carbonate deposits. The latter, used with the initial atmospheric 14 C activities reconstructed with the new IntCal09 calibration data, allows high-resolution age estimation of the gour calcite samples and their growth rates. The carbonate deposit grew between 9530 and 6635 yr cal BP (for dcp = 10.7 ± 1.8%; 2 s) or between 8518 and 5489 yr cal BP (for dcp = 20.5 ± 1.9%; 2 s). This coincides with humid periods that can be related to the Atlantic period in Europe and to Sapropel 1 in the eastern Mediterranean Sea. However, geomorphological changes at the cave entrance might also have played a role in the gour development. In the 1940's, when humans entered the cave for the first time since its prehistoric occupation, the calcite gours had already been inactive for several thousand years. (authors)

  15. Numerical simulation of formation and preservation of Ningwu ice cave, Shanxi, China

    Science.gov (United States)

    Yang, S.; Shi, Y.

    2015-10-01

    Ice caves exist in locations where annual average air temperature is higher than 0 °C. An example is Ningwu ice cave, Shanxi Province, the largest ice cave in China. In order to quantitatively investigate the mechanism of formation and preservation of the ice cave, we use the finite-element method to simulate the heat transfer process at this ice cave. There are two major control factors. First, there is the seasonal asymmetric heat transfer. Heat is transferred into the ice cave from outside very inefficiently by conduction in spring, summer and fall. In winter, thermal convection occurs that transfers heat very efficiently out of the ice cave, thus cooling it down. Secondly, ice-water phase change provides a heat barrier for heat transfer into the cave in summer. The calculation also helps to evaluate effects of global warming, tourists, colored lights, climatic conditions, etc. for sustainable development of the ice cave as a tourism resource. In some other ice caves in China, managers have installed airtight doors at these ice caves' entrances with the intention of "protecting" these caves, but this in fact prevents cooling in winter and these cave ices will entirely melt within tens of years.

  16. A cave response to environmental changes in the Late Pleistocene: a study of Budimirica Cave sediments, Macedonia

    Czech Academy of Sciences Publication Activity Database

    Temovski, M.; Pruner, Petr; Hercman, H.; Bosák, Pavel

    2016-01-01

    Roč. 69, č. 3 (2016), s. 307-316 ISSN 1330-030X Institutional support: RVO:67985831 Keywords : cave sediments * palaeoenvironmental changes * Late Pleistocene * Budimirica Cave * Macedonia Subject RIV: DB - Geology ; Mineralogy Impact factor: 0.595, year: 2016 http://www. geologia -croatica.hr/ojs/index.php/GC

  17. Radon in the Creswell Crags Permian limestone caves

    International Nuclear Information System (INIS)

    Gillmore, G.K.; Phillips, P.S.; Denman, A.R.; Gilbertson, D.D.

    2002-01-01

    An investigation of radon levels in the caves of Creswell Crags, Derbyshire, an important Site of Special Scientific Interest (SSSI) shows that the Lower Magnesian Limestone (Permian) caves have moderate to raised radon gas levels (27-7800 Bq m -3 ) which generally increase with increasing distance into the caves from the entrance regions. This feature is partly explained in terms of cave ventilation and topography. While these levels are generally below the Action Level in the workplace (400 Bq m -3 in the UK), they are above the Action Level for domestic properties (200 Bq m -3 ). Creswell Crags has approximately 40,000 visitors per year and therefore a quantification of effective dose is important for both visitors and guides to the Robin Hood show cave. Due to short exposure times the dose received by visitors is low (0.0016 mSv/visit) and regulations concerning exposure are not contravened. Similarly, the dose received by guides is fairly low (0.4 mSv/annum) due in part to current working practice. However, the risk to researchers entering the more inaccessible areas of the cave system is higher (0.06 mSv/visit). This survey also investigated the effect of seasonal variations on recorded radon concentration. From this work summer to winter ratios of between 1.1 and 9.51 were determined for different locations within the largest cave system

  18. Calculating flux to predict future cave radon concentrations

    Czech Academy of Sciences Publication Activity Database

    Rowberry, Matthew David; Martí, Xavier; Frontera, C.; Van De Wiel, M.J.; Briestenský, Miloš

    2016-01-01

    Roč. 157, JUN (2016), 16-26 ISSN 0265-931X R&D Projects: GA MŠk LM2010008 Institutional support: RVO:67985891 ; RVO:68378271 Keywords : cave radon concentration * cave radon flux * cave ventilation * radioactive decay * fault slip * numerical modelling Subject RIV: DC - Siesmology, Volcanology, Earth Structure; BG - Nuclear, Atomic and Molecular Physics, Colliders (FZU-D) Impact factor: 2.310, year: 2016

  19. Cave exploitation by an usual epigean species: a review on the current knowledge on fire salamander breeding in cave

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    Raoul Manenti

    2017-11-01

    Full Text Available The fire salamander (Salamandra salamandra is a relatively common epigean amphibian, widely distributed throughout Europe, which usually gives birth to aquatic larvae. Even if epigean streams represent the most common places in which the species breeds, in some countries caves with underground waters are also used. To improve our understanding of the habitat features allowing successful breeding of salamanders in underground sites, we combined an exhaustive review of the available literature, especially the grey one, with direct observations performed from 2008 to 2017 in several natural and artificial caves of Lombardy, Liguria and Tuscany (Italy, Ariège and Provence (France. We provide a synthesis of published and unpublished caves in which the fire salamander breeding has been observed, along with a synthesis of the investigated ecological, behavioural and morphological traits. The use of underground sites is reported in several published papers and appears to be a common phenomenon not limited to single karst areas. The absence of predators, the relative stability of the aquatic habitats and the possibility to exploit new ecological resources are environmental factors that favour the breeding of the fire salamander. Our synthesis suggests that breeding of fire salamanders in caves is not a random event, but a widespread phenomenon that may be linked to specific biogeographical factors. Further insights may be obtained by performing genetic analyses on both cave and epigean populations, and considering larger landscape scales for ecological studies as well. Gene flow between salamanders that breed in caves and in streams probably occurs, but on the other hand, assortative mating might limit it, thus allowing the conservation of local adaptations driving successful cave colonisation.

  20. Sources of sulphate minerals in limestone cave-a possible evidence of anthropogenic activity: a case study in Črna Jama Cave (Slovenia).

    Science.gov (United States)

    Jarc, Simona; Miler, Miloš; Šebela, Stanka; Zupančič, Nina

    2017-12-01

    In the caves, the formation of cave minerals is a consequence of a variety of chemical reactions, some of them also due to human activity. There are many caves in Slovenia, but sulphate minerals are not very often reported and analysed. In this study, the presence of sulphate minerals is detected by SEM/EDS analysis of speleothems from Črna Jama, a cave near Kočevje (southern Slovenia). The cave is characterised by its dark, almost black colour on cave walls, floor and speleothems. Anthropogenic influence in the cave is still visible, including the remains of a fireplace, some inscriptions on the walls and wooden containers. The analyses of some of the black-coated speleothems reveal the presence of calcium sulphate, confirmed by XRD as gypsum. Gypsum crystals are around 50 μm in size, and they occur in thin crusts. Additionally, some rare authigenic baryte crystals a few micrometres in size are detected. The sulphates δ 34 S value in gypsum found on dark coloured speleothems is + 10.4‰ Vienna Canyon Diablo Troilite (VCDT), while the sulphate δ 34 S of the bedrock is + 8.6‰ VCDT. The more likely source of sulphate ions is thus biomass burning rather than bedrock. Also, bedrock and biomass ash are a very probable source of calcium and barium. The highly probable pyrogenous origin of sulphates draws attention to human impact on cave mineralogy.

  1. Cryogenic Minerals in Caves of the Vizhay River (Northern Urals

    Directory of Open Access Journals (Sweden)

    E. P. Bazarova

    2018-03-01

    Full Text Available New information on the cryogenic mineral formations at the two Vizhay River caves (Northern Urals is given.   Calcite with the insignificant gypsum admixture predominates in the cryogenic material composition from both caves. In addition, the metastable phases of calcite, such as monohydrocalcite and ikaite were found. In the Saksofon Cave, calcite forms both spherulites and complanate grains. In Lednikovaya Cave, the major part of cryomaterial is presented by spherulites, which may suggests the significant supersaturation of solution. In Lednikovaya Сave, the distinct concentric structure with the growth zones denotes the cryogenic material formation in a thin water film under the partial thawing of upper part of long-term ice mound in summer. In Saksofon Cave the growth zones in crystals are poorly developed that probably caused by the seasonal glaciation in the cave and cryogenic minerals are younger than those in the Lednikovaya Cave.

  2. A geochronological approach for cave evolution in the Cantabrian Coast (Pindal Cave, NW Spain)

    Science.gov (United States)

    Jimenez-Sanchez, M.; Bischoff, J.L.; Stoll, H.; Aranburu, A.

    2006-01-01

    Some of the oldest speleothems in the North Cantabrian Coast (Spain) are reported for the first time in this work. Pindal Cave is developed at 24 m above sea level, in a karstic massif reaching its highest surface in a marine terrace (rasa) located at 50-64 m above the present sea level. Several phases of evolution were previously recognized into the cave, including block collapse of the roof, episodic flooding and detrital sedimentation, and chemical precipitation of at least four speleothem generations over both alluvial and collapse deposits. Three of these speleothem generations have been dated by U/Th. The first generation yielded ages from 124,2 ?? 1, 5 ka BP to 73,1 ?? 0,9 ka BP, giving a minimum age for the main detritic sediments in the cave. The second one is not dated. The third generation gives an age of 3,71 ?? 0,4 ka BP (mathematically corrected to 2.7 ?? 0.5 ka BP), while for the youngest generation, with actively growing stalagmites in the cave, basal ages of 200 years BP are estimated by counting annual laminae. The data suggest a tentative maximum elevation rate close to 0, 2 mm/yr for the Cantabrian Margin in this area, although further chronological studies will be needed to check this hypothesis. ?? 2006 Gebru??der Borntraeger.

  3. 3N Cave: Longest salt cave in the world

    Czech Academy of Sciences Publication Activity Database

    Bruthans, J.; Filippi, Michal; Zare, M.; Asadi, N.; Vilhelm, Z.

    2006-01-01

    Roč. 64, č. 9 (2006), s. 10-18 ISSN 0027-7010 R&D Projects: GA AV ČR(CZ) KJB301110501 Institutional research plan: CEZ:AV0Z30130516 Keywords : salt cave * salt karst * Iran * expedition Namak Subject RIV: DB - Geology ; Mineralogy

  4. Magnetic Susceptibility and Heavy Metals in Guano from South Sulawesi Caves

    Science.gov (United States)

    Rifai, H.; Putra, R.; Fadila, M. R.; Erni, E.; Wurster, C. M.

    2018-04-01

    Measurement of some magnetic properties have been performed on vertical profile from South Sulawesi caves (Mampu and Bubau) by using low cost, rapid, sensitive and non destructive magnetic method. The aim is to attempt to use magnetic characters as a fingerprint for anthropogenic pollution in the caves. Guano samples were collected every 5 cm at a certain section of Mampu and Bubau cave, South Sulawesi, starting from surface through 300 cm in depth of mampu Cave and 30 cm of Bubau Cave. The magnetic parameters such as magnetic susceptibility and percentage frequency dependence susceptibility were measured using the Bartington MS2-MS2B instruments and supported by X-Ray Fluoroscence (XRF) to know their element composition. The results show that the samples had variations in magnetic susceptibility from 3.5 to 242.6 x 10‑8 m3/kg for Mampu Cave and from 8.6 to 106.5 x 10‑8 m3/kg for Bubau Cave and also magnetic domain. Then, the XRF results show that the caves contain several heavy metals. Magnetic and heavy metal analyses showing that the magnetic minerals in caves are lithogenic (Fe-bearing minerals) in origin and anthropogenic (Zn content) in the caves.

  5. Differences between caves with and without bats in a Brazilian karst habitat

    Directory of Open Access Journals (Sweden)

    Camila G. Torquetti

    2017-10-01

    Full Text Available ABSTRACT. Since bats shelter in roosts during their period of diurnal inactivity, the quality and availability of roosts are important aspects of their ecology. Karst areas have great potential for the availability of day roosts, since they form caves, which serve as bat shelters. Here we characterize the caves used by bats in a preserved karst area of Southeastern Brazil. Using logistic regression analysis we identified the cave characteristics that influence bat occupation. Sixty-six caves were characterized based on measurements of internal height and width, height and width of the entrance(s of the cave, number of entrances, maximum horizontal development of cave, and internal temperature and humidity. In nineteen months we found 14 species in 32 caves. Most species were eventually recorded in multiple caves, with the exception of D. rotundus, G. soricina and A. planirostris, which were always found in the same caves. Desmodus rotundus showed maternity roost fidelity. We found no differences in microclimate between the caves that are occupied and those that are not. In other words, the microclimate of the caves studied herein can be characterized as stable over the years. The only predictor affecting the presence of bats in the study area was the cave’s maximum horizontal development: the caves that are occupied have greater horizontal development. Based on our results, we conclude that bats occupy many of the caves and that some species are more frequent in certain caves than in others, including some roosts that are used as maternity roosts. These findings indicate that these caves are important resources for the bats in the karst environment studied, and should be preserved.

  6. Ancient lineage, young troglobites: recent colonization of caves by Nesticella spiders.

    Science.gov (United States)

    Zhang, Yuanyuan; Li, Shuqiang

    2013-09-04

    The evolution and origin of cave organisms is a recurring issue in evolutionary studies, but analyses are often hindered by the inaccessibility of caves, morphological convergence, and complex colonization processes. Here we investigated the evolutionary history of Nesticella cave spiders, which are mainly distributed in the Yunnan-Guizhou Plateau, China. With comprehensive sampling and phylogenetic and coalescent-based analyses, we investigated the tempo and mode of diversification and the origins of these troglobites. We also aimed to determine which factors have influenced the diversification of this little-known group. Coalescent-based species delimitation validated the 18 species recognized by morphological inspection and also suggested the existence of cryptic lineages. Divergence time estimates suggested that Nesticella cave spiders in the Yunnan-Guizhou Plateau constituted a monophyletic troglobite clade that originated in the middle Miocene (11.1-18.6 Ma). Although the Yunnan-Guizhou Plateau clade was composed exclusively of troglobite species, suggesting an ancient common subterranean ancestor, we favor multiple, independent cave colonizations during the Pleistocene over a single ancient cave colonization event to explain the origin of these cave faunas. The diversification of plateau Nesticella has been greatly influenced by the sequential uplift of the plateau and likely reflects multiple cave colonizations over time by epigean ancestors during Pleistocene glacial advances. We concluded that plateau cave Nesticella represent an ancient group of spiders, but with young troglobite lineages that invaded caves only recently. The absence of extant epigean relatives and nearly complete isolation among caves supported their relict status. Our work highlights the importance of comprehensive sampling for studies of subterranean diversity and the evolution of cave organisms. The existence of potentially cryptic species and the relict status of Nesticella

  7. Energy and speleogenesis: Key determinants of terrestrial species richness in caves.

    Science.gov (United States)

    Jiménez-Valverde, Alberto; Sendra, Alberto; Garay, Policarp; Reboleira, Ana Sofia P S

    2017-12-01

    The aim of this study was to unravel the relative role played by speleogenesis (i.e., the process in which a cave is formed), landscape-scale variables, and geophysical factors in the determination of species richness in caves. Biological inventories from 21 caves located in the southeastern Iberian Peninsula along with partial least square (PLS) regression analysis were used to assess the relative importance of the different explanatory variables. The caves were grouped according to the similarity in their species composition; the effect that spatial distance could have on similarity was also studied using correlation between matrices. The energy and speleogenesis of caves accounted for 44.3% of the variation in species richness. The trophic level of each cave was the most significant factor in PLS regression analysis, and epigenic caves (i.e., those formed by the action of percolating water) had significantly more species than hypogenic ones (i.e., those formed by the action of upward flows in confined aquifers). Dissimilarity among the caves was very high (multiple-site β sim  = 0.92). Two main groups of caves were revealed through the cluster analysis, one formed by the western caves and the other by the eastern ones. The significant-but low-correlation found between faunistic dissimilarity and geographical distance ( r  =   .16) disappeared once the caves were split into the two groups. The extreme beta-diversity suggests a very low connection among the caves and/or a very low dispersal capacity of the species. In the region under study, two main factors are intimately related to the richness of terrestrial subterranean species in caves: the amount of organic material (trophic level) and the formation process (genesis). This is the first time that the history of a cave genesis has been quantitatively considered to assess its importance in explaining richness patterns in comparison with other factors more widely recognized.

  8. Cave-dwelling pholcid spiders (Araneae, Pholcidae: a review

    Directory of Open Access Journals (Sweden)

    Bernhard A. Huber

    2018-06-01

    Full Text Available Pholcidae are ubiquitous spiders in tropical and subtropical caves around the globe, yet very little is known about cave-dwelling pholcids beyond what is provided in taxonomic descriptions and faunistic papers. This paper provides a review based on a literature survey and unpublished information, while pointing out potential biases and promising future projects. A total of 473 native (i.e. non-introduced species of Pholcidae have been collected in about 1000 caves. The large majority of cave-dwelling pholcids are not troglomorphic; a list of 86 troglomorphic species is provided, including 21 eyeless species and 21 species with strongly reduced eyes. Most troglomorphic pholcids are representatives of only two genera: Anopsicus Chamberlin & Ivie, 1938 and Metagonia Simon, 1893. Mexico is by far the richest country in terms of troglomorphic pholcids, followed by several islands and mainland SE Asia. The apparent dominance of Mexico may partly be due to collectors’ and taxonomists’ biases. Most caves harbor only one pholcid species, but 91 caves harbor two and more species (up to five species. Most troglomorphic pholcids belong to two subfamilies (Modisiminae, Pholcinae, very few belong to Smeringopinae and Arteminae, none to Ninetinae. This is in agreement with the recent finding that within Pholcidae, microhabitat changes in general are concentrated in Modisiminae and Pholcinae.

  9. Trichodina nobilis Chen, 1963 and Trichodina reticulata Hirschmann et Partsch, 1955 from ornamental freshwater fishes in Brazil

    Directory of Open Access Journals (Sweden)

    ML Martins

    Full Text Available In the present work Trichodina reticulata and T. nobilis (Ciliophora: Trichodinidae are morphologically characterised from ornamental freshwater fish culture in the State of Santa Catarina, Brazil. The prevalence of infection and a list of comparative measurements are discussed. We examined "southern platyfish" Xiphophorus maculatus (n = 35, "goldfish" Carassius auratus (n = 31, "guppy" Poecilia reticulata (n = 20, "sailfin molly" Poecilia latipinna (n = 6, "beta" Betta splendens (n = 2 and "spotted headstander" Chilodus punctatus (n = 1. After being anesthetised in a benzocaine solution, fishes were examined for parasitological evaluation. A total of 51.57% fishes were parasitised by Trichodina spp. Carassius auratus was the most parasitised species, followed by X. maculatus and P. reticulata. Beta splendens, C. punctatus and P. latipinna were not parasitised by any trichodinid species. Two species of Trichodina were collected from the skin of fish: T. nobilis was found in C. auratus, P. reticulata and X. maculatus and T. reticulata was only observed in C. auratus. The importance of adequate handling in ornamental fish culture are also discussed.

  10. The effect of growth regulators on post-harvest Alchemilla mollis (Bauser Rothm. leaf longevity

    Directory of Open Access Journals (Sweden)

    Janowska Beata

    2016-12-01

    Full Text Available Leaves of Alchemilla mollis (Bauser Rothm. were the subject of the study. The leaves were harvested early in the morning from the department’s ornamental plant collection. Selected leaves were fully-developed and showed no signs of damage or discolouring. Gibberellic acid (GA3, benzyladenine (BA, meta-methoxytopolin (MemT and its riboside (MemTR at concentrations of 25, 50 and 75 mg dm−3 were applied in the form of solutions to four-hour leaf-conditioning in the room at a temperature of 18-20°C. After conditioning, the leaves were placed in distilled water. Leaves put into distilled water immediately after cutting served as the control. The post-harvest longevity of leaves of Alchemilla mollis was 7.2-11.8 days. The conditioning of leaves in gibberellic acid solutions at concentrations of 25-50 mg dm−3, benzyladenine at concentrations of 25 mg dm−3 and meta-methoxytopolin and its riboside at concentrations of 75 mg dm−3 extended the post-harvest longevity of leaves by 10.1-81.9%. The conditioning of leaves in gibberellic acid at a concentration of 50 mg dm−3 inhibited the degradation of chlorophyll, as indicated by the highest SPAD index values.

  11. {sup 14}C AMS dating Yongcheon cave

    Energy Technology Data Exchange (ETDEWEB)

    Lee, J.H., E-mail: jefflee@snu.ac.kr [AMS Lab., NCIRF, Seoul National University, Seoul 151-742 (Korea, Republic of); Choe, K. [AMS Lab., NCIRF, Seoul National University, Seoul 151-742 (Korea, Republic of); Kim, J.C. [Dept. of Physics and Astronomy, Seoul National University, Seoul 151-742 (Korea, Republic of); Choi, S.H.; Kang, J.; Song, S.; Song, Y.M. [AMS Lab., NCIRF, Seoul National University, Seoul 151-742 (Korea, Republic of); Jang, J.G. [Jeju National Museum, Jeju 690-782 (Korea, Republic of)

    2013-01-15

    The biggest island in South Korea is Jeju Island, which lies 80 km south of the mainland and has one shield volcano, Mt. Halla. The volcanic island and its lava tubes were added to the world heritage list by UNESCO in 2007. Among the many lava tubes on the island, a unique cave had been accidentally found in 2005 while some workers were replacing a telephone pole. Until the discovery, it had been completely isolated from the outside by naturally-built sand blocks. Yongcheon cave is a lime-decorated lava tube showing both the properties of a volcanic lava tube and a limestone cave. This cave, about 3 km in length, is acknowledged to be the best of this type in the world and includes a large clean-water lake, lava falls, and richly developed speleothems inside it. Even though there is archaeological evidence from well preserved pottery that ancient people entered this place, the preservation of artifacts was ensured by a geological change that made later entrance difficult. We have collected charcoal samples scattered around the cave and dated them using AMS. Ages were in the range of ca. 1570-1260 BP (A.D. 340-880) and this corresponds to the Ancient Three Kingdoms and the Unified Silla era in Korean history. The {sup 14}C AMS measurement results presented in this paper on wood charcoal provide precise dates which will be very useful not only to clarify the nature of human activities in this cave but also to provide reference dates when comparing other dating methods.

  12. CaveMan Enterprise version 1.0 Software Validation and Verification.

    Energy Technology Data Exchange (ETDEWEB)

    Hart, David

    2014-10-01

    The U.S. Department of Energy Strategic Petroleum Reserve stores crude oil in caverns solution-mined in salt domes along the Gulf Coast of Louisiana and Texas. The CaveMan software program has been used since the late 1990s as one tool to analyze pressure mea- surements monitored at each cavern. The purpose of this monitoring is to catch potential cavern integrity issues as soon as possible. The CaveMan software was written in Microsoft Visual Basic, and embedded in a Microsoft Excel workbook; this method of running the CaveMan software is no longer sustainable. As such, a new version called CaveMan Enter- prise has been developed. CaveMan Enterprise version 1.0 does not have any changes to the CaveMan numerical models. CaveMan Enterprise represents, instead, a change from desktop-managed work- books to an enterprise framework, moving data management into coordinated databases and porting the numerical modeling codes into the Python programming language. This document provides a report of the code validation and verification testing.

  13. Radon concentration measurements in the desert caves of Saudi Arabia

    Energy Technology Data Exchange (ETDEWEB)

    Al-Mustafa, Hanan [Women College, P. O. Box 838, Dammam 31113 (Saudi Arabia); Al-Jarallah, M.I. [Department of Physics, King Fahd University of Petroleum and Minerals, Dhahran 31261 (Saudi Arabia)]. E-mail: mibrahim@kfupm.edu.sa; Fazal-ur-Rehman [Department of Physics, King Fahd University of Petroleum and Minerals, Dhahran 31261 (Saudi Arabia); Abu-Jarad, F. [Radiation Protection Unit, Environmental Protection Department, Saudi Aramco P.O. Box 13027, Dhahran 31311 (Saudi Arabia)

    2005-11-15

    Beneath the harsh deserts of Saudi Arabia lie dark chambers and complex mazes filled with strange shapes and wondrous beauty. Radon concentration measurements have been carried out in the desert caves of Al-Somman Plateau in the Eastern Province of Saudi Arabia. Passive radon dosimeters, based on alpha particle etch track detectors with an inlet filter, were used in this study. A total of 59 dosimeters were placed in five caves for a period of six months. Out of 59 dosimeters, 37 could be collected for analysis. Measurements showed significant variations in radon concentrations in caves depending upon their natural ventilation. The results of the study show that the average radon concentration in the different caves ranges from 74 up to 451Bqm{sup -3}. The average radon concentration in four of the caves was low in the range 74-114Bqm{sup -3}. However, one cave showed an average radon concentration of 451Bqm{sup -3}. Radon is not a problem for tourists in the majority of caves. However, sometimes it may imply some limitation to the working time of guides.

  14. Radon concentration measurements in the desert caves of Saudi Arabia

    International Nuclear Information System (INIS)

    Al-Mustafa, Hanan; Al-Jarallah, M.I.; Fazal-ur-Rehman; Abu-Jarad, F.

    2005-01-01

    Beneath the harsh deserts of Saudi Arabia lie dark chambers and complex mazes filled with strange shapes and wondrous beauty. Radon concentration measurements have been carried out in the desert caves of Al-Somman Plateau in the Eastern Province of Saudi Arabia. Passive radon dosimeters, based on alpha particle etch track detectors with an inlet filter, were used in this study. A total of 59 dosimeters were placed in five caves for a period of six months. Out of 59 dosimeters, 37 could be collected for analysis. Measurements showed significant variations in radon concentrations in caves depending upon their natural ventilation. The results of the study show that the average radon concentration in the different caves ranges from 74 up to 451Bqm -3 . The average radon concentration in four of the caves was low in the range 74-114Bqm -3 . However, one cave showed an average radon concentration of 451Bqm -3 . Radon is not a problem for tourists in the majority of caves. However, sometimes it may imply some limitation to the working time of guides

  15. The architectural form of Qikou Cave dwellings in Chinese "Earth" culture

    Science.gov (United States)

    Chen, Xuanchen; Feng, Xinqun

    2018-03-01

    Cave building is not only a kind of architecture with unique style, but also a manifestation of Chinese traditional culture. Cave culture is an important part of Chinese traditional culture. The main purpose of this thesis which studies the architectural form of Qikou Cave, is to analyze how the cave building plays a positive role in promoting the development and application of modern resources and in cultural transmission. Based on a large amount of literature material, and taking Qikou Cave as an example, by studying the morphological characteristics of cave building, the paper takes an optimistic outlook on its future development and the sustainable development of the resources. It is expected that the cave culture can be further explored to promote the traditional Chinese culture and to drive the development of modern construction industry and resource conservation.

  16. Seasonal Temperature Variations controlling Cave Ventilation Processes in Cueva Larga, Puerto Rico

    Science.gov (United States)

    Winter, A.; Vieten, R.; Warken, S. F.; Schrӧder-Ritzrau, A.; Miller, T. E.; Scholz, D.

    2016-12-01

    Two years of monthly monitoring result in much better understanding of ventilation processes in caves. Cueva Larga, a tropical cave in Puerto Rico is 1440 m long with a large main passage (about 116,000 m3). Cave air pCO2 in the main passage varied seasonally, between 600 ppm in winter and 1800 ppm in summer. The seasonal variability in cave pCO2 made it possible to estimate a cave air exchange time of 36±5 days and a winter ventilation rate of 3,200±800 m3/day for the main cave passage. Calculations of virtual temperature and differences between cave and surface temperature show that the seasonal temperature cycle is the main driver of the alternation between a well-ventilated winter mode and a near-stagnant summer mode. The winter mode is characterized by a positive buoyancy contrast at night leading to maximal cave ventilation, while during summer ventilation is at a minimum. Between winter and summer, a transitional mode of partial cave ventilation is observed. On shorter time scales (diurnal to weekly), cave pCO2 is also influenced by atmospheric pressure but this variation is one order of magnitude lower than the seasonal pCO2 change. The cave morphology of Cueva Larga including its large volume, tubular shape and the obstructed cave entrance geometry are important boundary conditions for the observed ventilation patterns. Our findings emphasize that cave systems with varying morphology have to be studied individually in order to correctly describe ventilation processes.

  17. A world review of fungi, yeasts, and slime molds in caves

    Directory of Open Access Journals (Sweden)

    McAlpine Donald F.

    2013-01-01

    Full Text Available We provide a review of fungi, yeasts, and slime molds that have been found in natural solution caves and mines worldwide. Such habitats provide frequent roost sites for bats, and in eastern North America the environmental conditions that support white-nose syndrome, a lethal fungal disease currently devastating bat populations. A list of 1029 species of fungi, slime moulds, and yeasts in 518 genera have been documented from caves and mines worldwide in 225 articles. Ascomycota dominate the cave environment. Most research has been conducted in temperate climates, especially in Europe. A mean of 17.9±24.4SD fungal species are reported per study. Questions remain about the origin and ecological roles of fungi in caves, and which, if any, are cave-specialists. In the northern hemisphere, caves are generally characterized by relatively stable, low temperatures and a lack of organic substrates. This environment favors communities of oligotrophic, psychrotolerant fungi. Data that may help explain how cave environmental features and faunas inf luence the introduction and transmission of cave fungi remains scant.

  18. World Register of marine Cave Species (WoRCS): a new Thematic Species Database for marine and anchialine cave biodiversity

    NARCIS (Netherlands)

    Gerovasileiou, Vasilis; Martínez, Alejandro; Álvarez, Fernando; Boxshall, Geoff; Humphreys, William F.; Jaume, Damià; Becking, L.E.; Muricy, Guilherme; Hengstum, van Peter J.; Dekeyzer, Stefanie; Decock, Wim; Vanhoorne, Bart; Vandepitte, Leen; Bailly, Nicolas; Iliffe, Thomas M.

    2016-01-01

    Scientific exploration of marine cave environments and anchialine ecosystems over recent decades has led to outstanding discoveries of novel taxa, increasing our knowledge of biodiversity. However, biological research on underwater caves has taken place only in a few areas of the world and relevant

  19. A radon survey performed in caves in Slovenia

    International Nuclear Information System (INIS)

    Jovanovic, P.

    2002-01-01

    A survey of radon and radon decay product concentrations in several caves in a limestone region in Slovenia was initiated in 1986. In the period from 1989 to 1998, monthly surveys were undertaken in several caves which are open to tourists or used for speleotherapy purposes. The reason for carrying out these surveys, were dose estimates obtained for the guides and medical staff working in the caves. Daily average radon gas concentration determined ranged from several 100 Bq/m 3 up to 27 kBq/m 3 . Higher values were measured in the summer period. The equilibrium factors derived ranged from 0.05 to 0.89, with the higher values being measured in the winter period in vertical caves. In horizontal caves (with two entrances located opposite one another) these values ranged between 0.55 and 0.89. Annual doses estimated on the basis of various lung models ranged from 10 mSv to 85 mSv per year and per 2000 working hours. A significant difference was observed between the doses estimated by means of dosimetric models, and those estimated on the basis of the epidemiological model presented in ICRP 65. The value for the unattached fraction indicated in ICRP 65 is about 3%, but our measurements performed in the caves yielded higher values of up to 15%, with this highest value being determined in the Postojna cave. In the coming years, we will perform measurements to obtain the values for concentrations of unattached particles of radon daughters and values for particle-size distribution in the 3 different caves with the highest occupancy times for visitors. There are no regulations in force in Slovenia affecting exposures to elevated radon and radon daughter concentrations among underground workers. The health inspectorate can impose radiation monitoring measures for the purposes of performing dose calculations for underground workers. The results from such monitoring measures will contribute to the establishment of an ordinance regulating the performance of measurements at low

  20. Study of aerosols collected in a speleotherapeutic cave situated below Budapest, Hungary

    Energy Technology Data Exchange (ETDEWEB)

    Kertesz, Zs. E-mail: zsofi@moon.atomki.hu; Borbely-Kiss, I.; Hunyadi, I

    1999-04-02

    The Szemlohegy-cave is one of the well-known hydrothermal caves of the Rozsadomb area of Budapest, which have been used for speleotherapy of respiratory diseases for years. It is known from the periodically changing airborne radon activity concentration data, that airflow of seasonally reversed direction are formed along the cave passages and fissures due to the temperature difference between the surface and cave air. This means that an intensive interaction takes place between the cave and its environment. The pollution of nearby waters and the urban atmospheric air represents a real danger for these caves below Buda, which recently became the part of the UNESCO World Heritage. The study of cave aerosols should be very important from the point of view of either the control possibilities of the environmental impact or speleotherapy, and probably helps in getting acquainted with the cave-forming processes. In this work we applied our standard aerosol sampling method to the high-humidity environment of the caves, and we studied the elemental composition, size fractionation as well as the spatial distribution and the seasonal variation of cave aerosols. Thanks to the sensitivity of PIXE traces of anthropogenic pollution of the Budapest air are shown in the Szemlohegy-cave. Measured elemental concentrations remained less than one-tenth the air quality standard valid for the increasingly protected areas.

  1. Study of aerosols collected in a speleotherapeutic cave situated below Budapest, Hungary

    International Nuclear Information System (INIS)

    Kertesz, Zs.; Borbely-Kiss, I.; Hunyadi, I.

    1999-01-01

    The Szemlohegy-cave is one of the well-known hydrothermal caves of the Rozsadomb area of Budapest, which have been used for speleotherapy of respiratory diseases for years. It is known from the periodically changing airborne radon activity concentration data, that airflow of seasonally reversed direction are formed along the cave passages and fissures due to the temperature difference between the surface and cave air. This means that an intensive interaction takes place between the cave and its environment. The pollution of nearby waters and the urban atmospheric air represents a real danger for these caves below Buda, which recently became the part of the UNESCO World Heritage. The study of cave aerosols should be very important from the point of view of either the control possibilities of the environmental impact or speleotherapy, and probably helps in getting acquainted with the cave-forming processes. In this work we applied our standard aerosol sampling method to the high-humidity environment of the caves, and we studied the elemental composition, size fractionation as well as the spatial distribution and the seasonal variation of cave aerosols. Thanks to the sensitivity of PIXE traces of anthropogenic pollution of the Budapest air are shown in the Szemlohegy-cave. Measured elemental concentrations remained less than one-tenth the air quality standard valid for the increasingly protected areas

  2. Chemical deposits in volcanic caves of Argentina

    Directory of Open Access Journals (Sweden)

    Carlos Benedetto

    1998-01-01

    Full Text Available During the last Conference of the FEALC (Speleological Federation of Latin America and Caribbean Islands which was held in the town of Malargue, Mendoza, in February 1997, two volcanic caves not far from that town were visited and sampled for cave mineral studies. The first cave (Cueva del Tigre opens close to the Llancanelo lake, some 40 kms far from Malargue and it is a classical lava tube. Part of the walls and of the fallen lava blocks are covered by white translucent fibres and grains. The second visited cave is a small tectonic cavity opened on a lava bed some 100 km southward of Malargue. The cave “El Abrigo de el Manzano” is long no more than 10-12 meters with an average width of 3 meters and it hosts several bird nests, the larger of which is characterized by the presence of a relatively thick pale yellow, pale pink flowstone. Small broken or fallen samples of the secondary chemical deposits of both these caves have been collected in order to detect their mineralogical composition. In the present paper the results of the detailed mineralogical analyses carried out on the sampled material are shortly reported. In the Cueva del Tigre lava tube the main detected minerals are Sylvite, Thenardite, Bloedite and Kieserite, all related to the peculiar dry climate of that area. The flowstone of “El Abrigo de el Manzano” consists of a rather complex admixture of several minerals, the large majority of which are phosphates but also sulfates and silicates, not all yet identified. The origin of all these minerals is related to the interaction between bird guano and volcanic rock.

  3. Cave dwellings in the Mediterranean basin

    Directory of Open Access Journals (Sweden)

    Viedma Urdiales Eugenia María

    2011-01-01

    Full Text Available The construction and use of subterranean caves for different functions has been relatively commonplace throughout history in different regions around the world, especially in the Mediterranean basin. Some of them are still standing at the beginning of the 21st century, and are a good example of adaptation to the geographic environment, and a part of the historical heritage. Following a short overview of the different Mediterranean countries, this work pays special attention to the present use of caves as dwelling spaces in Italy, and particularly in Spain where the caves are currently in an interesting process of renovation to meet the needs of the present population. This process is helping to boost the local economy, and it is funded by both private and public sources in several towns in Andalusia (Spain.

  4. Evolution and adaptation of marine annelids in interstitial and cave habitats

    DEFF Research Database (Denmark)

    Martinez Garcia, Alejandro

    relatives in cave subterranean ecological refugia. Active colonization and ecological speciation to particular cave niches has been alternatively suggested, but the evaluation of that scenario is obscured by the dominance of crustaceans in anchialine habitats, ecologically similar out and inside caves....... The main goal of this thesis is to explore the evolutionary processes behind colonization and adaptation to submarine cave ecosystems in the Atlantic Ocean using annelids as a model, mainly when they involved ancestrally interstitial forms. In order to do that, we studied selected lineages of annelids...... with cave and interstitial representatives, mainly the families Protodrilidae, Nerillidae, Saccocirridae and Scalibregmatidae. The studies combined the characterization of ancestral and cave habitats, with morphological investigations and phylogenetic analyses founded on extensive taxon coverage...

  5. Stratigraphy, U-Th chronology, and paleoenvironments at Gladysvale Cave: insights into the climatic control of South African hominin-bearing cave deposits.

    Science.gov (United States)

    Pickering, Robyn; Hancox, Phillip J; Lee-Thorp, Julia A; Grün, Rainer; Mortimer, Graham E; McCulloch, Malcolm; Berger, Lee R

    2007-11-01

    Gladysvale Cave is one of the few Plio-Pleistocene hominin-bearing cave sites in South Africa that contains a well-stratified cave fill with clastic sediments interspersed with flowstones. The clastic sediments can be divided into units based on the presence of intercalated flowstones, forming flowstone bounded units (FBU). Ten MC-ICP-MS uranium-series dates on several flowstone horizons in the Gladysvale Internal Deposit fan indicate deposition from the late mid-Pleistocene ( approximately 570 ka) to Holocene ( approximately 7 ka) during limited periods of higher effective moisture. Clastic sedimentation occurred during the interceding, presumably more arid, periods. This sequence is not consistent with earlier models for South African caves that simply assumed interglacial sedimentation and glacial erosion. (13)C/(12)C data suggest that flowstone tended to form during periods with higher proportions of C(3) plants in the local vegetation, while clastic sediments reflect higher proportions of C(4) grasses, although this is not always the case. We argue that flowstones are precipitated during periods of higher effective precipitation and restricted cave entrances, while clastic sediments accumulated during periods with more open vegetation. The sedimentary fill of the fossiliferous deposits are, therefore, highly episodic in nature, with large periods of time unlikely to be represented. This has serious implications for the other hominin-bearing caves close by, as these deposits are likely to be similarly episodic. This is especially pertinent when addressing extinction events and reconstructions of paleoenvironments, as large periods of time may be unrecorded. The Gladysvale Cave fill sediments may serve as a climatically forced chronostratigraphic model for these less well-stratified and well-dated Plio-Pleistocene sites.

  6. Anti-Inflammatory Activity and Changes in Antioxidant Properties of Leaf and Stem Extracts from Vitex mollis Kunth during In Vitro Digestion

    Directory of Open Access Journals (Sweden)

    Juan Alfredo Morales-Del-Rio

    2015-01-01

    Full Text Available Vitex mollis is used in traditional Mexican medicine for the treatment of some ailments. However, there are no studies on what happens to the anti-inflammatory activity or antioxidant properties and total phenolic content of leaves and stem extracts of Vitex mollis during the digestion process; hence, this is the aim of this work. Methanolic, acetonic, and hexanic extracts were obtained from both parts of the plant. Extract yields and anti-inflammatory activity (elastase inhibition were measured. Additionally, changes in antioxidant activity (DPPH and ABTS and total phenols content of plant extracts before and after in vitro digestion were determined. The highest elastase inhibition to prevent inflammation was presented by hexanic extracts (leaf = 94.63% and stem = 98.30%. On the other hand, the major extract yield (16.14%, antioxidant properties (ABTS = 98.51% and DPPH = 94.47% of inhibition, and total phenols (33.70 mg GAE/g of dried sample were showed by leaf methanolic extract. Finally, leaf and stem methanolic extracts presented an antioxidant activity increase of 35.25% and 27.22%, respectively, in comparison to their initial values after in vitro digestion process. All samples showed a decrease in total phenols at the end of the digestion. These results could be the basis to search for new therapeutic agents from Vitex mollis.

  7. The CAVE (TM) automatic virtual environment: Characteristics and applications

    Science.gov (United States)

    Kenyon, Robert V.

    1995-01-01

    Virtual reality may best be defined as the wide-field presentation of computer-generated, multi-sensory information that tracks a user in real time. In addition to the more well-known modes of virtual reality -- head-mounted displays and boom-mounted displays -- the Electronic Visualization Laboratory at the University of Illinois at Chicago recently introduced a third mode: a room constructed from large screens on which the graphics are projected on to three walls and the floor. The CAVE is a multi-person, room sized, high resolution, 3D video and audio environment. Graphics are rear projected in stereo onto three walls and the floor, and viewed with stereo glasses. As a viewer wearing a location sensor moves within its display boundaries, the correct perspective and stereo projections of the environment are updated, and the image moves with and surrounds the viewer. The other viewers in the CAVE are like passengers in a bus, along for the ride. 'CAVE,' the name selected for the virtual reality theater, is both a recursive acronym (Cave Automatic Virtual Environment) and a reference to 'The Simile of the Cave' found in Plato's 'Republic,' in which the philosopher explores the ideas of perception, reality, and illusion. Plato used the analogy of a person facing the back of a cave alive with shadows that are his/her only basis for ideas of what real objects are. Rather than having evolved from video games or flight simulation, the CAVE has its motivation rooted in scientific visualization and the SIGGRAPH 92 Showcase effort. The CAVE was designed to be a useful tool for scientific visualization. The Showcase event was an experiment; the Showcase chair and committee advocated an environment for computational scientists to interactively present their research at a major professional conference in a one-to-many format on high-end workstations attached to large projection screens. The CAVE was developed as a 'virtual reality theater' with scientific content and

  8. Census of biodiversity in marine caves of the eastern Mediterranean Sea

    Directory of Open Access Journals (Sweden)

    V. GEROVASILEIOU

    2015-03-01

    Full Text Available Scientific information on the biodiversity of marine caves in the eastern Mediterranean is limited, especially when considering the extensively studied caves of the north-western and central Mediterranean. Aiming to enhance current knowledge regarding cave communities, this study represents a first assessment of the marine cave biota of the eastern Mediterranean, as this has been defined by the European Union’s Marine Strategy Framework Directive (MSFD. Information retrieved from an extensive overview of relevant scientific documents was combined with original data recorded from 23 marine caves of the north-eastern Mediterranean. Our results report a total of 520 taxa recorded in eastern Mediterranean marine caves to date, the majority of which are sponges, polychaetes, rhodophytes, bivalves, fishes, and gastropods. These include several protected, endemic, and alien species. However, not all taxonomic groups have been equally studied among different areas and future studies are expected to raise the number of endemic and alien species. The overall observed trend is that the reported species number is generally related to sampling effort and scientific expertise. The most well-studied marine cave communities of the eastern Mediterranean are those of the Aegean Sea (especially its northern sector, which presented the highest number of species, followed by those of the Levantine. Furthermore, our research in Aegean caves revealed numerous new records for the marine cave fauna of the eastern basin, while several species are reported for the first time in the marine cave habitat. The critical need for further scientific research, monitoring, and conservation of this unique ecosystem was highlighted by (i the presence of certain species endemic to the eastern Mediterranean coupled with a high proportion of alien species, especially in the Levantine, and (ii the marine cave habitat availability in isolated insular areas of the eastern Mediterranean.

  9. Chronology of guitarrero cave, peru.

    Science.gov (United States)

    Lynch, T F; Gillespie, R; Gowlett, J A; Hedges, R E

    1985-08-30

    Dating by accelerator mass spectrometry of wooden artifacts, cord, and charcoal samples from Guitarrero Cave, Peru, supports the antiquity of South America's earliest textiles and other perishable remains. The new dates are consistent with those obtained from disintegration counters and leave little doubt about the integrity of the lower Preceramic layers and their early cultivars. Re-evaluation of the mode of deposition suggests that most of the remains resulted from short-term use of the cave in the eighth millennium B.C., with a possible brief human visit as early as 12,560 years ago.

  10. Development of New Microsatellite DNA Markers from Apostichopus japonicus and Their Cross-Species Application in Parastichopus parvimensis and Pathallus mollis

    Directory of Open Access Journals (Sweden)

    Guiping Chen

    2011-09-01

    Full Text Available Twenty microsatellite DNA markers were developed for sea cucumber and used to investigate polymorphisms of 60 wild Apostichopus japonicus individuals collected from China. It revealed that all the markers were polymorphic. A total of 164 alleles were detected at 20 loci. The number of alleles per locus varied from 3 to 17 with an average of 8.2, and the expected heterozygosities of each locus ranged from 0.03 to 0.89 with an average of 0.64. Cross-species amplification was also conducted in Parastichopus parvimensis collected from the United States and Pathallus mollis collected from Peru. The result showed that 17 loci amplified Parastichopus parvimensis DNAs while only 4 loci could amplify Pathallus mollis DNAs. All of the polymorphic markers would be useful for future genetic breeding and the assessment of genetic variation within sea cucumbers.

  11. Radon in New Zealand tourist caves

    International Nuclear Information System (INIS)

    Lyons, R.G.; Solomon, S.B.; Langroo, R.

    1998-01-01

    Seasonal average radon concentrations in 112 sites in 22 NZ tourist caves have been measured using track etch detectors over an annual cycle. Values ranged from -3 to nearly 10,000 Bq m -3 . Seasonal variation was also very marked with factors of over 50 for the same site in different seasons being recorded. Thirty six percent of the sites exceeded the ICRP guideline of 1000 Bq m -3 at which action to reduce exposure is recommended. Caves are fragile ecological and chemical systems, therefore, may be seriously adversely affected by standard techniques to reduce radon concentrations. They are also complex physical systems, and an understanding of the principles and parameters governing cave aerodynamics is essential when considering options to reduce exposure. This paper discusses possible causes for variations in radon concentrations observed in this study and the implications for viable actions

  12. Occurrence of sea spider Endeis mollis Carpenter (Arthropoda: Pycnogonida on the test panels submerged in Gulf of Mannar, southeast coast of India

    Directory of Open Access Journals (Sweden)

    S. Satheesh

    2012-06-01

    Full Text Available Sea spiders (Pycnogonids are exclusively marine arthropods with worldwide distribution. Pycnogonida remains one of the poorly investigated groups encountered in fouling communities. In the present study, distribution pycnogonid species Endeis mollis associated with the fouling community developed on test panels submerged at Kudankulam coast, Gulf of Mannar was studied for a period of two years. Throughout the period of investigation, Endeis mollis was observed on the test panels. A maximum of 55 individuals per square dm was observed during pre-monsoon season and a minimum of 9 individuals per square dm during monsoon season. Results of this study on seasonal distribution are of considerable interest because so little has been documented on the ecology of Pycnogonids in India.

  13. Radon measurements using track detector in Wadi Sannur cave

    International Nuclear Information System (INIS)

    Shahin, F.; Eissa, M.F.; Mostafa, R.M.

    2008-01-01

    The most important contributors to the committed effective dose received by population due to natural sources are the short-lived decay products of radon ( 222 Rn). In natural voids, such as caves, most radon will enter the system from diffusion across the rock-air interface. It is well known that factors such as air pressure and temperature control the distribution of radon. The radon concentration measurements in the Wadi Sannur cave in Beni-Suef governorate in the period from 14 th of July 2005 to 17 th of October 2005. The average radon concentrations in the right, left and whole first cave are 916.12 ± 179.09, 819.63 ± 54.72 and 873.90± 147.11 Bq m -3 respectively. The measurements were performed using track etch detector of type Cr-39. After exposure, all detectors were etched chemically in 6.25 M NaOH solution at 70 C degrees for 6 h. The tracks were counted with an optical microscope magnifying 400 times. The average temperature inside the first cave during the period of measurements is 25-26 C degrees. The annual effective doses for the workers and visitors in the cave have been calculated. The average radon concentration in the Wadi Sannur cave, was 873.90 ± 147.11 Bq m -3 . The annual effective doses for worker and visitor in the cave were 1.33 ± 0.24 and 0.041 ± 0.007 mSv respectively. The doses are within the international recommended dose of 1.15 mSv. (author)

  14. Exposure of tour guides to Radon at the Cango caves

    International Nuclear Information System (INIS)

    Pule, O.J.; Lindsay, R.

    2008-01-01

    Full text: A study was commissioned by the National Nuclear Regulator of South Africa to investigate the radon levels in the Cango caves and the associated radiological exposure to tour guides. The Cango caves are about 1.3 km in length and are visited by about 300000 tourists per annum due to their natural beauty. This study followed an earlier investigation by the Department of Health which indicated levels in excess of 1000 Bqm -3 which could lead to excess exposure of the cave tour guides. Radon in the various cave chambers and radon exposure of tour guides was measured using RAD-7 continuous radon monitor, electrets ion chambers and personnel monitoring electrets respectively. The measurements in the cave were done during summer and winter seasons to determine any variations between the seasons. The occupancy time for individual guides and equilibrium factor were also investigated. The radon concentration in the cave range from 1000 Bqm -3 to more than 2000 Bqm -3 with equilibrium factor of approximately 0.4, and the variation between winter and summer measurements are insignificant. The radon exposure levels to tour guides differ due to various time periods they spent in the caves. The average dose to tour guides due to radon is 7 mSv -a and the highest exposure is about 10 mSv -a . The exposure to tourists is found to be insignificant due to time they spent in the cave. The regulatory authority is currently investigating what action is necessary to protect the tour guides. (author)

  15. The WP-CAVE concept for an underground high-level nuclear waste repository

    International Nuclear Information System (INIS)

    Pettersson, G.

    1984-02-01

    A central, nearly spherical cave, of diameter 40 m is excavated in rock and the waste fuel is placed in it. The fuel canisters are placed in cylindrical holes in large concrete balls which are stored at the bottom of a central stack in the cave. Other empty balls fill the rest of the cave. By natural convection, the heat is evenly distributed in the cave and the surrounding central rock body. A clay barrier, which completely surrounds the rock body, prevents ground water circulation for a very long time and protects the cave against tectonic movements. The cave can store approx. 350 tons of fuel after the 10 years of intermediate storage

  16. Forensic Fluid Dynamics and the Indian Spring (1991) cave collapse problem

    Science.gov (United States)

    Nof, D.

    2013-05-01

    The collapse of the Indian spring cave (Florida) in 1991 was unique because it occurred while cave divers were in the cave. For the most part, the submerged cave is large enough to accommodate a passing truck so the cave divers were not in touch with its walls and it is hard to imagine why would it naturally collapse just when the divers were in it. Recently, Nof and Paldor (2010) resolved this apparent paradox by suggesting that resonance in the air pockets in the cavern, created by breathing (open circuit) divers, may have contributed to the collapse. In this scenario, divers present in the cavern during the dive may have (unknowingly) caused the collapse through the pressurized air/gas that they release with each breath. When the breathing period of the diver(s) matches the natural oscillations period of the "cave oscillator", the ensuing resonance causes the air pressure in the pockets to increase uncontrollably. Here, we place the above theory on a more solid ground. To do so, we first extended the resonance theory from our original two-pockets, symmetrical U-tube model (with two identical branches that were not specifically identified within the cave system) to a one (identified) pocket in the cavern and a very broad basin (identified, of course) that serves as the other branch of the U-tube. Our methodology is to apply familiar fluid dynamics principles to the situation that occurred in the cave. We did so, step-by-step, on the basis of our interviews with four out of the five surviving cave-divers. Namely, we dissected their testimonies to arrive at a physically plausible scenario determined on basis of a fluid dynamics application to the natural flow in the cave and the flow induced by the compressed air released by the divers as well as the collapsed mud. We found that the oscillation period was larger than what we earlier calculated (still relevant to the case, nevertheless), and that, in contrast to what most cave divers believe, there was a temporary

  17. Analysis of the Condition and Development Opportunities of Cave Tourism in Primorsko-Goranska County

    Directory of Open Access Journals (Sweden)

    Rade Knežević

    2011-04-01

    Full Text Available This paper examines caves as morphological factors of tourism development in Primorsko-Goranska County. The primary aim is to promote cave heritage parallel with the development of cave tourism. The methodological framework is based on analyzing material in the archives of tourist boards, taking an inventory of cave resources (case study, conducting interviews, and making a SWOT analysis of cave tourism development. Research results show that caves represent a complex resource in the tourist trade of Primorsko-Goranska County, to which little importance has been attached up to date (priority being given to mass tourism. In the context of tourism development, caves fall into four groups. The first group comprises caves that have a long tradition, but show signs of aging as a tourism product. The second group includes caves that were once open to the public, but were later closed for some reason. The third group consists of caves on the seaside or seabed visited by speleologists-divers. The fourth group is made up of potential cave sites that have been evaluated as being attractive, but is located in hard-to-access areas, making their valorisation in tourism purpose more difficult. Only Lokvarka Cave has a substantial influence on the development of special interest tourism in Lokve, while in all other cases, the role of caves in the tourism offering needs to be revalorised.

  18. The potential of perennial cave ice in isotope palaeoclimatology

    International Nuclear Information System (INIS)

    Yonge, Charles J.; MacDonald, William D.

    1999-01-01

    Perennial ice from caves on and to the east of the Canadian Great Divide yield delta O 18 and delta D values which are usually high measurements where compared with the average precipitation for the region. Furthermore, these ice data fall below and along lines of lower slope than the Global Meteoric Water Line. To explain the observed relationships, we propose the following process. a vapour-ice isotopic fractionation mechanism operates on warm season vapour when it precipitates as hoar ice on entering the caves. The subsequent fall of hoar to the cave floor through mechanical overloading along with ice derived from ground-water seepage (with a mean annual isotopic composition), results in massive ice formation of a mixed composition. This mixed composition is what is observed in the characteristic relationships found here. Such findings suggest that a warm versus cold climate interpretation for ancient cave ice may be the opposite of that found in the more familiar polar and glacial ice caves. (Author) 3 figs., 1 tab., 12 refs

  19. Caving thickness effects of surrounding rocks macro stress shell evolving characteristics

    Institute of Scientific and Technical Information of China (English)

    XIE Guang-xiang; YANG Ke

    2009-01-01

    In order to explore the influence of different caving thicknesses on the MSS dis-tribution and evolving characteristics of surrounding rocks in unsymmetrical disposal and fully mechanized top-coal caving (FMTC), based on unsymmetrical disposal characteris-tics, the analyses of numerical simulation, material simulation and in-situ observation were synthetically applied according to the geological and technical conditions of the 1151(3) working face in Xieqiao Mine. The results show that the stress peak value of the MSS-base and the ratio of MSS-body height to caving thickness are nonlinear and inversely proportional to the caving thickness. The MSS-base width, the MSS-body height, the MSS-base distance to working face wall and the rise distance of MSS-base beside coal pillar are nonlinear and directly proportional to the caving thickness. The characteristics of MSS distribution and its evolving rules of surrounding rocks and the integrated caving thickness effects are obtained. The investigations will provide lots of theoretic references to the surrounding rocks' stability control of the working face and roadway, roadway layout, gas extraction and exploitation, and efficiency of caving, etc.

  20. The biogeochemistry of anchialine caves: Progress and possibilities

    Science.gov (United States)

    Pohlman, John W.

    2011-01-01

    Recent investigations of anchialine caves and sinkholes have identified complex food webs dependent on detrital and, in some cases, chemosynthetically produced organic matter. Chemosynthetic microbes in anchialine systems obtain energy from reduced compounds produced during organic matter degradation (e.g., sulfide, ammonium, and methane), similar to what occurs in deep ocean cold seeps and mud volcanoes, but distinct from dominant processes operating at hydrothermal vents and sulfurous mineral caves where the primary energy source is mantle derived. This review includes case studies from both anchialine and non-anchialine habitats, where evidence for in situ chemosynthetic production of organic matter and its subsequent transfer to higher trophic level metazoans is documented. The energy sources and pathways identified are synthesized to develop conceptual models for elemental cycles and energy cascades that occur within oligotrophic and eutrophic anchialine caves. Strategies and techniques for testing the hypothesis of chemosynthesis as an active process in anchialine caves are also suggested.

  1. Morphology and evolution of sulphuric acid caves in South Italy

    Science.gov (United States)

    D'Angeli, Ilenia M.; De Waele, Jo; Galdenzi, Sandro; Madonia, Giuliana; Parise, Mario; Vattano, Marco

    2016-04-01

    Sulphuric acid speleogenesis (SAS) related to the upwelling of acid water enriched in H2S and CO2 represents an unusual way of cave development. Since meteoric infiltration waters are not necessarily involved in speleogenesis, caves can form without the typical associated karst expressions (i.e. dolines) at the surface. The main mechanism of sulphuric acid dissolution is the oxidation of H2S (Jones et al., 2015) which can be amplified by bacterial mediation (Engel et al., 2004). In these conditions, carbonate dissolution associated with gypsum replacement, is generally believed to be faster than the normal epigenic one (De Waele et al., 2016). In Italy several SAS caves have been identified, but only few systems have been studied in detail: Frasassi and Acquasanta Terme (Marche)(Galdenzi et al., 2010), Monte Cucco (Umbria) (Galdenzi & Menichetti, 1995), and Montecchio (Tuscany) (Piccini et al., 2015). Other preliminary studies have been carried out in Calabria (Galdenzi, 2007) and Sicily (De Waele et al., 2016). Several less studied SAS cave systems located in South Italy, and in particular in Apulia (Santa Cesarea Terme), Sicily (Acqua Fitusa, Acqua Mintina) and Calabria (Mt. Sellaro and Cassano allo Ionio) have been selected in the framework of a PhD thesis on SAS caves and their speleogenesis. Using both limestone tablet weight loss (Galdenzi et al., 2012) and micro erosion meter (MEM) (Furlani et al., 2010) methods the dissolution rate above and under water in the caves will be quantified. Geomorphological observations, landscape analysis using GIS tools, and the analysis of gypsum and other secondary minerals (alunite and jarosite) (stable isotopes and dating) will help to reconstruct the speleogenetic stages of cave formation. Preliminary microbiological analysis will determine the microbial diversity and ecology in the biofilms. References Engel S.A., Stern L.A., Bennett P.C., 2004 - Microbial contributions to cave formation: New insight into sulfuric acid

  2. Speleothem (Cave Deposit) Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Records of past temperature, precipitation, and other aspects of climate derived from mineral deposits found in caves. Parameter keywords describe what was measured...

  3. Phylogeography of Sardinian cave salamanders (genus Hydromantes) is mainly determined by geomorphology.

    Science.gov (United States)

    Chiari, Ylenia; van der Meijden, Arie; Mucedda, Mauro; Lourenço, João M; Hochkirch, Axel; Veith, Michael

    2012-01-01

    Detecting the factors that determine the interruption of gene flow between populations is key to understanding how speciation occurs. In this context, caves are an excellent system for studying processes of colonization, differentiation and speciation, since they represent discrete geographical units often with known geological histories. Here, we asked whether discontinuous calcareous areas and cave systems represent major barriers to gene flow within and among the five species of Sardinian cave salamanders (genus Hydromantes) and whether intraspecific genetic structure parallels geographic distance within and among caves. We generated mitochondrial cytochrome b gene sequences from 184 individuals representing 48 populations, and used a Bayesian phylogeographic approach to infer possible areas of cladogenesis for these species and reconstruct historical and current dispersal routes among distinct populations. Our results show deep genetic divergence within and among all Sardinian cave salamander species, which can mostly be attributed to the effects of mountains and discontinuities in major calcareous areas and cave systems acting as barriers to gene flow. While these salamander species can also occur outside caves, our results indicate that there is a very poor dispersal of these species between separate cave systems.

  4. Palaeoclimate Research in Villars Cave (Dordogne, SW-France

    Directory of Open Access Journals (Sweden)

    Genty Dominique

    2008-10-01

    Full Text Available Villars Cave is a typical shallow cave from South-West France (45.44°N; 0.78°E; 175 m asl that has provided several speleothempalaeoclimatic records such as the millennial scale variability of the Last Glacial period and the Last Deglaciation. Monitoring theVillars cave environment over a 13-year period has helped in the understanding of the stable isotopic speleothem content and inthe hydrology. For example, it was demonstrated that most of the calcite CaCO3 carbon comes from the soil CO2, which explainsthe sensitivity of the δ13C to any vegetation and climatic changes. Drip rate monitoring, carried out under four stalactites from thelower and upper galleries, has shown a well marked seasonality of the seepage water with high flow rates during winter and spring.A time delay of about two months is observed between the water excess (estimated from outside meteorological stations and thedrip rate in the cave. A great heterogeneity in the flow rate amplitude variations and in the annual quantity of water between twonearby stalactites is observed, confirming the complexity of the micro-fissure network system in the unsaturated zone. At a dailyscale, the air pressure and drip rates are anti-correlated probably because of pressure stress on the fissure network. Cave air CO2concentration follows soil CO2 production and is correlated with its δ13C content. Since the beginning of the monitoring, the cave airtemperature, in both lower and upper galleries, displays a warming trend of ~+0.4°C±0.1/10yrs. This might be the consequence ofthe outside temperature increase that reaches the Villars Cave galleries through thermal wave conduction. Chemistry monitoringover a few years has shown that the seepage water of the lower gallery stations is significantly more concentrated in trace and minorelements (i.e. Sr, Mg, Ba, U than the upper stations, probably due to the 10-20 m depth difference between these galleries, whichimplies a different seepage pathway

  5. Determination of the Radon Concentration and Radioactivity Level in Karaca Cave

    International Nuclear Information System (INIS)

    Kara, A.

    2008-01-01

    In this study, the radon gas concentration in the Karaca cave which is open to tourism has been determined and the negative effects of radon gas on people were discussed. Karaca cave (Gumushane) is visited by many tourists every year. The measurements of radon gas concentration which affects the health of human beings negatively and even causes the lung cancer when it reaches high points have been done for the summer and winter season. LR-115 passive radon detector was used to determine radon concentrations in the cave both winter and summer season. The average radon concentration in the Karaca cave were determined as 823 Bq/m 3 and 1023 Bq/m 3 for the summer and winter season, respectively. Moreover, to find out the natural radioactivity in the cave, the gamma spectroscopic analysis of soil, stone and stalagmite samples were carried out and their relations with the radon gas in the cave atmosphere was analyzed

  6. Urban landscape and architectural workshop Škocjan Caves 2007

    Directory of Open Access Journals (Sweden)

    Andrej Mahovič

    2012-01-01

    Full Text Available The fundamental aim and purpose of the Škocjan Caves 2007 student workshop was for students participating in the seminar of the Faculty of Architecture in Ljubljana to recognise and address some specific spatial and design issues for the further sustainable development of the Škocjan Caves. The working hypothesis of the workshop was that, in spite of significant results in the protection and development of the Caves which have been achieved in the past ten years, it is still necessary to address some spatial issues through an academic process, and to offer different architectural and spatial solutions. The aforesaid is even more important as the Caves are recognised as the only natural and cultural heritage of the Republic of Slovenia to be listed as a UNESCO World Heritage site since 1986.

  7. Tamarugite from Diana Cave (SW Romania) -first true karst occurrence

    Science.gov (United States)

    Pušcaš, C. M.; Onac, B. P.; Effenberger, H. S.; Povarǎ, I.

    2012-04-01

    Diana Cave is located within the town limits of Baile Herculane (SW Romania) and develops as a 14 m long, westward oriented, unique passage guided by the Diana fault [1]. At the far end of the cave, the thermo-mineral Diana Spring wells forth. In the early 1970s a mine gallery that intersected the cave was created to drain the water into a pumping station and the original cave passage was somewhat altered and reinforced with concrete. Today the concrete and the silty limestone cave walls are heavily corroded by H2SO4 outgassing from the hot water (ca. 50°C) and display abundant gypsum crusts, soggy aggregates of native S, and a variety of more exotic sulfates. Among them, a mineral that has been previously identified in caves only in connection to volcanic activity, either as thermal springs or fumaroles [2]: tamarugite [NaAl(SO4)26H2O]. It was [3] that first mentioned the occurrence of this Na and Al sulfate in Diana Cave, our research aiming to give a detailed description of this mineral, its paragenesis, and mechanisms of precipitation. Recently, tamarugite has also been identified in a sulfuric acid cave from Greece [4]. Along with powder X-ray diffractions coupled with Rietveld refinement, scanning electron microscope, and electron probe micro-analysis, δ18O and δ34S compositions of the sulfate mineral as well as precipitates from the water were analyzed to identify and better constrain the genesis of this rare sulfate. Regrettably, the crystal size of our specimens is inappropriate for identification by means of single crystal X-ray diffraction. Physical and chemical parameters of Diana Spring were as well measured on several occasions. Geochemical analysis suggests that the minute, white tamarugite flakes precipitated in Diana Cave as a result of the interactions between the thermo-mineral water or water vapor and the original limestone bedrock and concrete that blankets the mine gallery. [1] Povara, I., Diaconu, G., Goran, C. (1972). Observations pr

  8. Depth Perception in Cave and Panorama

    DEFF Research Database (Denmark)

    Mullins, Michael; Strojan, Tadeja Zupancic

    2004-01-01

    This study compares aspects of spatial perception in a physical environment and its virtual representations in a CAVE and Panorama, derived from recent research. To measure accuracy of spatial perception, participants in an experiment were asked to look at identical objects in the three environme......, learning and training in virtual environments; in architectural education; and participatory design processes, in which the dialogue between real and imagined space may take place in virtual . reality environments...... environments and then locate them and identify their shape on scaled drawings.  Results are presented together with statistical analysis. In a discussion of the results, the paper addresses the two hypothetical assertions ? that depth perception in physical reality and its virtual representations in CAVE......This study compares aspects of spatial perception in a physical environment and its virtual representations in a CAVE and Panorama, derived from recent research. To measure accuracy of spatial perception, participants in an experiment were asked to look at identical objects in the three...

  9. Management in a neotropical show cave: planning for invertebrates conservation

    Directory of Open Access Journals (Sweden)

    Thais Giovannini Pellegrini

    2012-07-01

    Full Text Available Lapa Nova is a dolomitic cave about 4.5 km long located in northwestern Minas Gerais state, Brazil. The cave experiences intense tourism, concentrated over a single period of the year, during the Feast of Our Lady of Lapa. In order to evaluate the impacts felt by the invertebrate community from this tourism, a new methodology was proposed. Four types of areas (intense visitation area, outlying visitation areas, moderate visitation areas and no-visitation areas were sampled for invertebrates. There was one sampling prior and another on the last day of the 128th feast, to evaluate the effects of visitation on cave-dwelling invertebrates. Results show that invertebrate populations residing in more intensely visited areas of the cave undergo changes in distribution following the event. As a consequence of tourism, invertebrates shift to outlying locations from the visited area, which serve as refuges to the communities. Apparently, the fact that there are places inside Lapa Nova inaccessible to tourists reduces the impact suffered by the invertebrate community, as those sites serve as refuges for cave-dwelling organisms during the pilgrimage. A proper management plan was devised for the tourism/religious use of the cave. It consists basically of delimiting marked pathways for tourists, allowing invertebrates to seek shelter at locations outside visited areas and keeping no-visitation areas off-limits to tourism based on the results of the visitation effects on cave-dwelling invertebrates.

  10. Candidate cave entrances on Mars

    Science.gov (United States)

    Cushing, Glen E.

    2012-01-01

    This paper presents newly discovered candidate cave entrances into Martian near-surface lava tubes, volcano-tectonic fracture systems, and pit craters and describes their characteristics and exploration possibilities. These candidates are all collapse features that occur either intermittently along laterally continuous trench-like depressions or in the floors of sheer-walled atypical pit craters. As viewed from orbit, locations of most candidates are visibly consistent with known terrestrial features such as tube-fed lava flows, volcano-tectonic fractures, and pit craters, each of which forms by mechanisms that can produce caves. Although we cannot determine subsurface extents of the Martian features discussed here, some may continue unimpeded for many kilometers if terrestrial examples are indeed analogous. The features presented here were identified in images acquired by the Mars Odyssey's Thermal Emission Imaging System visible-wavelength camera, and by the Mars Reconnaissance Orbiter's Context Camera. Select candidates have since been targeted by the High-Resolution Imaging Science Experiment. Martian caves are promising potential sites for future human habitation and astrobiology investigations; understanding their characteristics is critical for long-term mission planning and for developing the necessary exploration technologies.

  11. Biomarkers of waterborne copper exposure in the guppy Poecilia vivipara acclimated to salt water

    Energy Technology Data Exchange (ETDEWEB)

    Machado, Anderson Abel de Souza [Universidade Federal do Rio Grande, Programa de Pós-Graduação em Oceanografia Biológica, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul (Brazil); Hoff, Mariana Leivas Müller [Universidade Federal do Rio Grande, Instituto de Ciências Biológicas, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul (Brazil); Klein, Roberta Daniele [Universidade Federal do Rio Grande, Programa de Pós-Graduação em Ciências Fisiológicas – Fisiologia Animal Comparada, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul (Brazil); Cardozo, Janaina Goulart [Universidade Federal do Rio Grande, Instituto de Ciências Biológicas, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul (Brazil); Giacomin, Marina Mussoi [Universidade Federal do Rio Grande, Programa de Pós-Graduação em Ciências Fisiológicas – Fisiologia Animal Comparada, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul (Brazil); Pinho, Grasiela Lopes Leães [Universidade Federal do Rio Grande, Instituto de Oceanografia, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul (Brazil); and others

    2013-08-15

    Highlights: •Acute effects of waterborne copper were evaluated in the estuarine guppy Poecilia vivipara. •Fishes were acutely exposed to waterborne copper in salt water. •Waterborne copper affects the response of several biochemical and genetic endpoints. •Catalase, reactive oxygen species, antioxidant capacity and lipid peroxidation are responsive to copper exposure. •Copper exposure induces DNA damages in fish erythrocytes. -- Abstract: The responses of a large suite of biochemical and genetic parameters were evaluated in tissues (liver, gills, muscle and erythrocytes) of the estuarine guppy Poecilia vivipara exposed to waterborne copper in salt water (salinity 24 ppt). Activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase, and glutathione S-transferase), metallothionein-like protein concentration, reactive oxygen species (ROS) content, antioxidant capacity against peroxyl radicals (ACAP), and lipid peroxidation (LPO) were evaluated in liver, gills, and muscle. Comet assay score and nuclear abnormalities and micronucleated cell frequency were analyzed in peripheral erythrocytes. The responses of these parameters were evaluated in fish exposed (96 h) to environmentally relevant copper concentrations (5, 9 and 20 μg L{sup −1}). In control and copper-exposed fish, no mortality was observed over the experimental period. Almost all biochemical and genetic parameters proved to be affected by waterborne copper exposure. However, the response of catalase activity in liver, ROS, ACAP and LPO in muscle, gills and liver, and DNA damages in erythrocytes clearly showed to be dependent on copper concentration in salt water. Therefore, the use of these parameters could be of relevance in the scope of biomonitoring programs in salt water environments contaminated with copper.

  12. Differential preservation of vertebrates in Southeast Asian caves

    Directory of Open Access Journals (Sweden)

    Julien Louys

    2017-09-01

    Full Text Available Caves have been an important source of vertebrate fossils for much of Southeast Asia, particularly for the Quaternary. Despite this importance, the mechanisms by which vertebrate remains accumulate and preserve in Southeast Asian caves has never been systematically reviewed or examined. Here, we present the results of three years of cave surveys in Indonesia and Timor-Leste, describing cave systems and their attendant vertebrate accumulations in diverse geological, biogeographical, and environmental settings. While each cave system is unique, we find that the accumulation and preservation of vertebrate remains are highly dependent on local geology and environment. These factors notwithstanding, we find the dominant factor responsible for faunal deposition is the presence or absence of biological accumulating agents, a factor directly dictated by biogeographical history. In small, isolated, volcanic islands, the only significant accumulation occurs in archaeological settings, thereby limiting our understanding of the palaeontology of those islands prior to human arrival. In karstic landscapes on both oceanic and continental islands, our understanding of the long-term preservation of vertebrates is still in its infancy. The formation processes of vertebrate-bearing breccias, their taphonomic histories, and the criteria used to determine whether these represent syngenetic or multiple deposits remain critically understudied. The latter in particular has important implications for arguments on how breccia deposits from the region should be analysed and interpreted when reconstructing palaeoenvironments.

  13. X-ray analysis of aerosol samples from a therapeutic cave

    Energy Technology Data Exchange (ETDEWEB)

    Alfoeldy, B. E-mail: alfi@sunserv.kfki.hu; Toeroek, Sz.; Kocsonya, A.; Szokefalvi-Nagy, Z.; Balla, Md.I

    2001-04-01

    Cave therapy is an efficient therapeutic method to cure asthma, the exact healing effect, however, is not clarified, yet. This study is motivated by the basic assumption that aerosols do play the key role in the cave therapy. This study is based on measurements of single aerosol particles originating from a therapeutic cave of Budapest, Hungary (Szemlohegyi cave). Aerosol particles have been collected in the regions arranged for the therapeutic treatment. Samples were further analysed for chemical and morphological aspects, determining the particle size distribution and classifying them according to elemental composition. Three particle classes have been detected based on major element concentration: alumino-silicate, quartz and calcium carbonate. Calcium ions have well-known physiological influence: anti-spastic, anti-inflammation and excretion reducing effects. Inflammation, accompanying spasm and extreme excretion production cause the smothering stigma, the so-called asthma. Therefore it could be assumed that calcium ions present in high concentration in the cave's atmosphere is the major cause of the healing effect.

  14. X-ray analysis of aerosol samples from a therapeutic cave

    International Nuclear Information System (INIS)

    Alfoeldy, B.; Toeroek, Sz.; Kocsonya, A.; Szokefalvi-Nagy, Z.; Balla, Md.I.

    2001-01-01

    Cave therapy is an efficient therapeutic method to cure asthma, the exact healing effect, however, is not clarified, yet. This study is motivated by the basic assumption that aerosols do play the key role in the cave therapy. This study is based on measurements of single aerosol particles originating from a therapeutic cave of Budapest, Hungary (Szemlohegyi cave). Aerosol particles have been collected in the regions arranged for the therapeutic treatment. Samples were further analysed for chemical and morphological aspects, determining the particle size distribution and classifying them according to elemental composition. Three particle classes have been detected based on major element concentration: alumino-silicate, quartz and calcium carbonate. Calcium ions have well-known physiological influence: anti-spastic, anti-inflammation and excretion reducing effects. Inflammation, accompanying spasm and extreme excretion production cause the smothering stigma, the so-called asthma. Therefore it could be assumed that calcium ions present in high concentration in the cave's atmosphere is the major cause of the healing effect

  15. WP-Cave - assessment of feasibility, safety and development potential

    International Nuclear Information System (INIS)

    1989-09-01

    According to SKB R and D-programme 1986, alternative disposal methods will be investigated to provide a basis for selecting a site and a repository system for the Swedish spent nuclear fuel. The present report is a comparison between the WP-Cave and the reference concept KBS-3. The comparison has resulted in the following conclusions: - Both concepts are judged to be able to provide adequate safety. - A utilization of the potential of the WP-Cave requires, however, extensive development in areas where the current state of knowledge and available data are incomplete. - The higher temperatures in the WP-Cave lead to greater uncertainty as to long-term performance. Reducing this uncertainty would require many yaers of research and substantial resources. - Both repositories, including the barriers they incorporate, could be built with a normal adaption of available technology. -It is not possible to say today whether it would be simpler to find suitable sites for one design or the other. - The WP-Cave is considerably more expensive. A future research direction based on a concentrated emplacement of spent fuel along the lines of the WP-Cave is therefore judged to entail greater uncertainty as regards the possibilities of achieving acceptable safety and to require greater resources for research and development, at the same time as the costs of building the repository would be higher. The studies of the WP-Cave as an integral system should therfore be discontinued. Certain barrier designs in the WP-Cave could also be utulized in repository designs with lower temperature, for example the reduction potential of the steel canisters and the hydraulic cage's diversion of groundwater. Studies within these areas are being conducted within SKB and should continue

  16. MDMA (ecstasy/molly) use among African Americans: The perceived influence of hip-hop/rap music.

    Science.gov (United States)

    Rigg, Khary K; Estreet, Anthony T

    2018-02-12

    Over the past two decades, the demographic profile of MDMA (ecstasy/molly) users has changed. In particular, African American MDMA use has risen in some cities. One explanation of this new trend is the drug's recent popularity (as molly) in hip-hop/rap (HHR) music. Several top rappers endorse the drug as a way to have fun or get women "loose." There are currently no studies, however, that investigate the extent to which African American MDMA users listen to HHR music or the influence that these pro-MDMA messages have on their use of the drug. To address this gap, the current study used survey data to (a) identify the extent to which HHR music is listened to by African American MDMA users and (b) assess the perceived influence of HHR music on their decision to begin using. Qualitative interview data are also presented to contextualize the influence of these messages on their use of MDMA. The findings of this study suggest that African American MDMA users are high consumers of HHR music and that pro-MDMA messages in HHR music are influencing their expectations of the drug and their decision to initiate use. These findings add to the limited amount of research on African American MDMA use and have the potential to inform future interventions.

  17. The role of natural ventilation in the exposure to radon in the Postojna Cave

    Science.gov (United States)

    Gregorič, A.; Smerajec, M.; Vaupotič, J.

    2012-04-01

    Postojna Cave is the biggest of 21 show caves in Slovenia and one of present day's most visited show caves in the world. Long and branched out cave system, large entrances at different levels, inflow of the Pivka river, and large variation of the outdoor air temperature and precipitation, make the Postojna Cave also a very complex climatic system in which each part shows different conditions. The cave is only naturally ventilated and it is therefore characterised by high radon concentration, which depends on the ventilation regime in different seasons, resulting in typical annual cycles of radon levels in the cave air. Postojna Cave is a typical horizontal cave, where the difference between outside and cave air temperature represents the main driving force for air circulation. In winter, when the cave temperature is higher than outside, cave air is released from the cave into the outdoor atmosphere due to the air draught caused by the 'chimney effect', thus allowing fresh and cold outdoor air to enter the cave through low lying openings. This effect is not operative in summer, when the outside temperature is higher than in the cave, and air draught is minimal or reversed. In addition, air circulation can be locally altered due to other processes, like changing level of Pivka river during the rainy season and local geomorphologic characteristics of cave passages. High radon concentration in the Postojna Cave is the reason for thorough studies of the methodology for dose estimates of the personnel working in the cave. Due to high relative humidity and low air circulation, the cave air is characterised by very low particle concentration, which play an important role in radon dosimetry. Therefore parallel monitoring of radioactive aerosols of radon decay products (RnDP) and general (non-radioactive) aerosols in the particle size range of 10-1100 nm was performed in the air of Postojna Cave at the lowest point of tourist path in summer, winter and both transitional

  18. Visualization framework for CAVE virtual reality systems

    OpenAIRE

    Kageyama, Akira; Tomiyama, Asako

    2016-01-01

    We have developed a software framework for scientific visualization in immersive-type, room-sized virtual reality (VR) systems, or Cave automatic virtual environment (CAVEs). This program, called Multiverse, allows users to select and invoke visualization programs without leaving CAVE’s VR space. Multiverse is a kind of immersive “desktop environment” for users, with a three-dimensional graphical user interface. For application developers, Multiverse is a software framework with useful class ...

  19. Investigation of Sediment Pathways and Concealed Sedimentological Features in Hidden River Cave, Kentucky

    Science.gov (United States)

    Feist, S.; Maclachlan, J. C.; Reinhardt, E. G.; McNeill-Jewer, C.; Eyles, C.

    2016-12-01

    Hidden River Cave is part of a cave system hydrogeologically related to Mammoth Cave in Kentucky and is a multi-level active cave system with 25km of mapped passages. Upper levels experience flow during flood events and lower levels have continuously flowing water. Improper industrial and domestic waste disposal and poor understanding of local hydrogeology lead to contamination of Hidden River Cave in the early 1940s. Previously used for hydroelectric power generation and as a source of potable water the cave was closed to the public for almost 50 years. A new sewage treatment plant and remediation efforts since 1989 have improved the cave system's health. This project focuses on sedimentological studies in the Hidden River Cave system. Water and sediment transport in the cave are being investigated using sediment cores, surface sediment samples and water level data. An Itrax core scanner is used to analyze sediment cores for elemental concentrations, magnetic susceptibility, radiography, and high resolution photography. Horizons of metal concentrations in the core allow correlation of sedimentation events in the cave system. Thecamoebian (testate amoebae) microfossils identified in surface samples allow for further constraint of sediment sources, sedimentation rates, and paleoclimatic analysis. Dive recorders monitor water levels, providing data to further understand the movement of sediment through the cave system. A general time constraint on the sediment's age is based on the presence of microplastic in the surface samples and sediment cores, and data from radiocarbon and lead-210 dating. The integration of various sedimentological data allows for better understanding of sedimentation processes and their record of paleoenvironmental change in the cave system. Sediment studies and methodologies from this project can be applied to other karst systems, and have important applications for communities living on karst landscapes and their water management policies.

  20. Uranium-series dating of fossil bones from alpine caves

    International Nuclear Information System (INIS)

    Leitner-Wild, E.; Steffan, I.

    1993-01-01

    During the course of an investigation of fossil cave bear populations the uranium-series method for absolute age determination has been applied to bone material. The applicability of the method to bone samples from alpine caves is demonstrated by the concordance of U/Th and U/Pa ages and cross-checks with the radiocarbon method. Stratigraphic agreement between bone ages and carbonate speleothem ages also indicates the potential of the uranium-series method as a suitable tool for the age determination of fossil bones from alpine cave environments. (Author)

  1. Modeling of luminance distribution in CAVE-type virtual reality systems

    Science.gov (United States)

    Meironke, Michał; Mazikowski, Adam

    2017-08-01

    At present, one of the most advanced virtual reality systems are CAVE-type (Cave Automatic Virtual Environment) installations. Such systems are usually consisted of four, five or six projection screens and in case of six screens arranged in form of a cube. Providing the user with a high level of immersion feeling in such systems is largely dependent of optical properties of the system. The modeling of physical phenomena plays nowadays a huge role in the most fields of science and technology. It allows to simulate work of device without a need to make any changes in the physical constructions. In this paper distribution of luminance in CAVE-type virtual reality systems were modelled. Calculations were performed for the model of 6-walled CAVE-type installation, based on Immersive 3D Visualization Laboratory, situated at the Faculty of Electronics, Telecommunications and Informatics at the Gdańsk University of Technology. Tests have been carried out for two different scattering distribution of the screen material in order to check how these characteristicinfluence on the luminance distribution of the whole CAVE. The basis assumption and simplification of modeled CAVE-type installation and results were presented. The brief discussion about the results and usefulness of developed model were also carried out.

  2. Tamarugite in the Steam-Condensate Alteration Paragenesis in Diana Cave (SW Romania)

    Science.gov (United States)

    Puscas, C. M.; Onac, B. P.; Effenberger, H. S.; Povară, I.

    2012-12-01

    The double-salt hydrate tamarugite [NaAl(SO4)2 6H2O] is an uncommon mineral in the cave environment, forming as a result of chemical reactions between water and bedrock only under very specific conditions. The Diana Cave hosts a unique tamarugite occurrence, the first one to be reported from a typical karst environment. The cave is located within the limits of Băile Herculane township in the Cerna Mountains, SW Romania. It consists of a 14 m long, westward-oriented single passage, developed along the Diana Fault. In 1974 a concrete-clad mine gallery was created to channel the thermal water (Diana 1+2 Spring) flowing through the cave to a pumping station. The spring's chemical and physical parameters fluctuated through time, averaging 51.98° C, discharge of 0.96 Ls-1, pH of 7.46, 5768.66 ppm TDS, 9303 μScm-1 conductivity, 5.02 salinity. The major chemical components of the thermo-mineral water in Diana Cave are, Na+ (1392.57 ppm), K+ (58.55 ppm), Ca2+ (725.16 ppm), Mg2+ (10.78 ppm), Cl- (3376.83 ppm), and SO42- (92.27 ppm), and H2S (24.05 ppm), with traces of Si, Fe2+, Br+, I-, and Li+. The general air circulation pattern within the cave is fairly simple: cold air from the outside sweeps into the cave along the floor, heats up at the contact with the thermo-mineral water, ascends, and exists the cave along the ceiling. At the contact with the cold walls of the Diana Cave, the hot steam condenses and gives rise to a rich and exotic sulfate-mineral paragenesis (including halotrichite-series minerals, gypsum, bassanite, anhydrite, epsomite, alunite, halite, native sulfur, etc.). The most exotic minerals precipitate at or below the contact between the Tithonic - Neocomian limestone and the overlaying Cretaceous shaly limestone, as a result of steam-condensate alteration. Minerogenetic mechanisms responsible for the peculiar sulfate mineral assemblage in Diana Cave are evaporation, oxidation, hydrolysis, double exchange reactions, and deposition from vapours or

  3. EFFECT OF LIGHT AND GIBERELLIC ACID (AG3) ON THE GERMINATION OF MINTHOSTACHYS MOLLIS (LABIATAE)

    OpenAIRE

    Fernández Alonso José Luis; Melgarejo Luz Marina; Suárez Peñaranda Diego Fernando

    2011-01-01

    Minthostachys mollis (Kunth) Griseb. o muña es un arbusto perenne que crece naturalmente en las región andina de Colombia; esta especie es ampliamente utilizada por las comunidades rurales en donde es valorada por sus propiedades medicinales para tratar diferentes afecciones y como control de larvas de insectos transmisores de enfermedades; sin embargo, las poblaciones naturales de esta planta han sido fuertemente reducidas por acción antrópica, por lo tanto, es necesario generar tecnologías...

  4. First assessment on the air CO2 dynamic in the show caves of tropical karst, Vietnam

    Directory of Open Access Journals (Sweden)

    Duc A. Trinh

    2018-01-01

    Full Text Available In this study, air, water, and host rock in show caves in a Vietnam’s karst region was monitored and analyzed to identify the ventilation regime and track the cave air CO2 sources. In general, the studied caves are well ventilated. In dynamic – multiple entrance caves, air ventilation is described with the use of U shape model. In static – single entrance cave, air circulation is explained by cold air trap model. Both ventilation models suggest that air is more circulated in winter than in summer. Seasonally, the cave air CO2 increases from early spring to summer. Value in the deepest part of the single-entrance cave is approximately 1,000 ppmv and 8,000 ppmv in early spring and summer, respectively. In multiple-entrance and wet caves, CO2 level is fairly constant all over the show section, increasing from 500 ppmv in early spring to 2,000 ppmv in summer. Data of microclimate, CO2 content, and particularly δ13C show that cave air, particularly in single entrance cave, has higher CO2 concentration during summer due to a stagnation of cave air circulation and an elevated CO2 input from soil and epikarst. The cave air CO2 increase is also observed after intense rainfalls. A factor that increase cave air CO2 in show caves during the festive days could probably be huma n exhaling but the extent of human factor in these studied cave systems should be further investigated. Cave waters including cave pools and streams mediate CO2 level in wet caves. Above all, the atmospheric fraction of CO2 is always dominant (>60% in all cave sections.

  5. Measurements of Aerosol Characteristics in Skocjan Caves

    International Nuclear Information System (INIS)

    Jovanovic, P.

    2013-01-01

    Measurements of radon concentration and radon progeny concentration (attached and unattached) have been performed in Skocjan caves. In the same time also aerosol concentration (PM 10 ), aerosol size distribution with ten stage Hauke impactor and Scanning Mobility Particle Sizer - SMPS have been performed. The idea was to find impact of outer air and visitors to the aerosol characteristics of cave air. Measurements with impactor have been implemented in summer and winter period, with SMPS only in summer period. Radon concentrations ranged in winter period in region from 500 to 1000 Bq/m 3 , equilibrium factor was about 55 %. In summer period radon concentration increased up to 10 kBq/m 3 , equilibrium factor was about 45 %, and unattached fraction went up to 20 %. Measurements of aerosol size distribution show lower aerosol sizes in winter season (around 1 μm) and bigger aerosol sizes in summer season (around 3 - 6 μm). We could not find good correlation between unattached fraction and aerosol size distribution. Also we could not find clear impact of visitors to the air characteristics in cave. Probably our measuring location was too close to the entrance and the impact of outer air was too high. We will repeat measurements deeper in cave to find better results.(author)

  6. The genesis of a lava cave in the Deccan Volcanic Province (Maharashtra, India

    Directory of Open Access Journals (Sweden)

    Nikhil R. Pawar

    2016-01-01

    Full Text Available Lava tubes and channels forming lava distributaries have been recognized from different parts of western Deccan Volcanic Province (DVP. Openings of smaller dimension have been documented from the pāhoehoe flows around Pune, in the western DVP. A small lava cave is exposed in Ghoradeshwar hill, near Pune. Detailed field studies of the physical characteristics, structure and morphology of the flows hosting the lava tube has been carried out. This is the first detailed documentation of a lava cave from the DVP. The lava cave occurs in a compound pāhoehoe flow of Karla Formation, characterized by the presence of lobes, toes and small scale features like squeeze-ups. Field observations and measurements reveal that the dimensions of the cave are small, with low roof and a maximum width of 108 cm. The cave morphology along the 20 m passage varies from circular to semi-circular, with a twilight zone to the north. The gentle micro-topography at Ghoradeshwar controlled the advancement of pāhoehoe lobes and toes within the sheet lobe. The pre-flow gradients towards the north led to the progression of flow from the east, where the cave opening is presently seen. Dimensions and related morphology of the lava cave suggest that it can be best described as a small sub-crustal cave formed by draining of an inflated of pāhoehoe lava lobe. At Ghoradeshwar, besides the natural lava cave, Buddhist caves carved in pāhoehoe lava flows are also observed, indicating that early man took advantage of the existing openings in pāhoehoe flows and sculpted the caves to suit their requirements.

  7. High Resolution Time Series Cave Ventilation Processes and the Effects on Cave Air Chemistry and Drip Waters: Speleoclimatology and Proxy Calibration

    Science.gov (United States)

    Kowalczk, A. J.; Froelich, P. N.; Gaffka, C.; Tremaine, D.

    2008-12-01

    Continuous high resolution (sub-hourly), long-term (Nov 2007-present) monitoring of cave air chemistry (Temperature, Relative Humidity, Barometric Pressure, Radon-222, CO2, Air flow, Wind speed and direction) in a shallow subtropical cave (Hollow Ridge) in N Florida reveals two major ventilation mechanisms: 1) ventilation driven by winds across the cave entrances, and 2) ventilation driven by density differences between atmospheric and cave air. The degree and type of ventilation strongly influence the 222Rn and CO2 of cave air, which in turn affects the timing and extent of calcite deposition in speleothems. The degree of ventilation is estimated using a cave air CO2-δ13CO2 Keeling Plot, or a simple radon deficiency model. Results show cave air has an atmospheric component ranging from 10-90%. During fall and winter, average CO2 (700 ppmv) and 222Rn (50-100 dpm/L) are lower than in spring and summer (CO2 = 1200 ppmv; 222Rn = 1000 dpm/L) due to increased winter ventilation. Decreased ventilation during the summer allows CO2 and 222Rn levels to rise. Winter daily ventilation is primarily a function of density gradients between cave air and atmospheric air, while summer daily ventilation is primarily a function of late morning NW-NE winds above the cave. Stable isotope analyses of drip water (fracture drip and pore flow drip) and aquifer water from Hollow Ridge agree with previous isotope studies of drip water at Florida Caverns State Park, 2 km to the NE. During summer, isotopic composition of pore flow drip water (δ18O -3.8 to -4.0 per mil; δD -17.3 to -20.2 per mil VSMOW) and aquifer water (δ18O -4.0 per mil; δD -18.0 to -21.1 per mil) are similar to average annual weighted isotopic composition of precipitation (δ18O -3.6 per mil) while fracture drip waters (δ18O -3 to -3.4 per mil; δD -11.9 to -14.3 per mil) likely reflect the isotopic composition of individual precipitation events. Pore flow drip waters δ18O are weakly correlated with drip rates

  8. Geophysical exploration in vicinity of the Unicorn Cave, South Harz Mountains, Germany

    Science.gov (United States)

    Kaufmann, Georg; Romanov, Douchko; Nielbock, Ralf

    2010-05-01

    The Unicorn Cave in the southern Harz Mountains in Germany is located in an outcrop of dolomite from the Zechstein formation, which is underlain by Grauwacke rocks. The cave, about 600 meters long, consists of several large chambers, which are connected by a gallery following the main fault alignments in E/W, NE/SW, and NW/SE direction. The overburden of the cave is shallow, between 10 and 30 m. We have used this cave site to perform a sensitivity test for both gravimetric and geoelectic methods above the cave. Additionally, geoelectic mapping has been used to assess the thickness of the cave sediments in one of the chambers. Our results show a clear signal in the Bouguer anomaly, which can only be explained by a combined model of the void space and the sediment filling. Geoelectric results are less clear, but support the gravimetry.

  9. Minerals cave, Volcan Irazu, Costa Rica: description, mineralogy and origin

    International Nuclear Information System (INIS)

    Ulloa, Andres; Campos-Fernandez, Cristian S.; Rojas, Leonardo

    2013-01-01

    Two caves are found in the NW sector of the main crater of the Volcan Irazu at the base of the crown of a glide. The caves are located in an area of structural weakness of the massif developed in sequences of pyroclasts with hydrothermal alteration. Several explorations are organized for the recognition of the caves. The purpose has been of collecting samples and photographs for mineralogical and topographic analyses, through X-ray diffractometry. The minerals present in the samples are compared and identified by means of PDF-2 power x-ray diffraction database of the 2007 ICDD, International Center for Diffraction Data. The origin of the caves has been studied, and it is suggested to carry out other of complement studies [es

  10. Isotopic investigations of the waters from the Movile Cave - Mangalia area

    International Nuclear Information System (INIS)

    Feurdean, Lucia; Feurdean, Victor

    2001-01-01

    As a conservative tracer in carbonate rocks deuterium was used to determine the unelucidated problems of water origin in the Movile Cave-Mangalia, which is the unique ecosystem from the world based on chemoautotrophic conditions. According to the δD values the water from Movile Cave is meteoric in origin but can not originate from local site. The groundwater from neighboring area of cave has their recharge area at high altitude and considerable distance. δD values of water samples present time variations with a distinct seasonal effect. The seasonal δD values are shifted with a half meteoric cycle vs. normal succession of seasonal maximum and minimum values. Water seems to be originated from the Prebalkan Plateau situated in the south of Dobrogea. The study indicates that the intrusion of water in the karst occurs by conduit flow and hydrostatic pressure. The geometry of conduit controls the movement of the water. The cave and the Karaoban Lake is the discharge area of two main components of groundwater: the first is coming from southwest and has isotope characteristic similar to lower altitude water (500 m) and the second is moving from southeast and has the isotope composition similar to high altitude water (>1000 m). The Movile Cave and Karaoban Lake are connected and the cave water is discharged by overflow mechanism isolating the cave from atmosphere. (authors)

  11. Speleogenesis, geometry, and topology of caves: A quantitative study of 3D karst conduits

    Science.gov (United States)

    Jouves, Johan; Viseur, Sophie; Arfib, Bruno; Baudement, Cécile; Camus, Hubert; Collon, Pauline; Guglielmi, Yves

    2017-12-01

    Karst systems are hierarchically spatially organized three-dimensional (3D) networks of conduits behaving as drains for groundwater flow. Recently, geostatistical approaches proposed to generate karst networks from data and parameters stemming from analogous observed karst features. Other studies have qualitatively highlighted relationships between speleogenetic processes and cave patterns. However, few studies have been performed to quantitatively define these relationships. This paper reports a quantitative study of cave geometries and topologies that takes the underlying speleogenetic processes into account. In order to study the spatial organization of caves, a 3D numerical database was built from 26 caves, corresponding to 621 km of cumulative cave passages representative of the variety of karst network patterns. The database includes 3D speleological surveys for which the speleogenetic context is known, allowing the polygenic karst networks to be divided into 48 monogenic cave samples and classified into four cave patterns: vadose branchwork (VB), water-table cave (WTC), looping cave (LC), and angular maze (AM). Eight morphometric cave descriptors were calculated, four geometrical parameters (width-height ratio, tortuosity, curvature, and vertical index) and four topological ones (degree of node connectivity, α and γ graph indices, and ramification index) respectively. The results were validated by statistical analyses (Kruskal-Wallis test and PCA). The VB patterns are clearly distinct from AM ones and from a third group including WTC and LC. A quantitative database of cave morphology characteristics is provided, depending on their speleogenetic processes. These characteristics can be used to constrain and/or validate 3D geostatistical simulations. This study shows how important it is to relate the geometry and connectivity of cave networks to recharge and flow processes. Conversely, the approach developed here provides proxies to estimate the evolution of

  12. Identification of Martian Cave Skylights Using the Temperature Change During Day and Night

    Directory of Open Access Journals (Sweden)

    Jongil Jung

    2014-06-01

    Full Text Available Recently, cave candidates have been discovered on other planets besides the Earth, such as the Moon and Mars. When we go to other planets, caves could be possible human habitats providing natural protection from cosmic threats. In this study, seven cave candidates have been found on Pavonis Mons and Ascraeus Mons in Tharsis Montes on Mars. The cave candidates were selected using the images of the Context Camera (CTX on the Mars Reconnaissance Orbiter (MRO. The Context Camera could provide images with the high resolution of 6 meter per pixel. The diameter of the candidates ranges from 50 to 100m. Cushing et al. (2007 have analyzed the temperature change at daytime and nighttime using the Thermal Emission Imaging System (THEMIS for the sites of potential cave candidates. Similarly, we have examined the temperature change at daytime and at nighttime for seven cave candidates using the method of Cushing et al. (2007. Among those, only one candidate showed a distinct temperature change. However, we cannot verify a cave based on the temperature change only and further study is required for the improvement of this method to identify caves more clearly.

  13. Identification of Martian Cave Skylights Using the Temperature Change During Day and Night

    Science.gov (United States)

    Jung, Jongil; Yi, Yu; Kim, Eojin

    2014-06-01

    Recently, cave candidates have been discovered on other planets besides the Earth, such as the Moon and Mars. When we go to other planets, caves could be possible human habitats providing natural protection from cosmic threats. In this study, seven cave candidates have been found on Pavonis Mons and Ascraeus Mons in Tharsis Montes on Mars. The cave candidates were selected using the images of the Context Camera (CTX) on the Mars Reconnaissance Orbiter (MRO). The Context Camera could provide images with the high resolution of 6 meter per pixel. The diameter of the candidates ranges from 50 to 100m. Cushing et al. (2007) have analyzed the temperature change at daytime and nighttime using the Thermal Emission Imaging System (THEMIS) for the sites of potential cave candidates. Similarly, we have examined the temperature change at daytime and at nighttime for seven cave candidates using the method of Cushing et al. (2007). Among those, only one candidate showed a distinct temperature change. However, we cannot verify a cave based on the temperature change only and further study is required for the improvement of this method to identify caves more clearly.

  14. Profiling bacterial diversity in a limestone cave of the western Loess Plateau of China

    Directory of Open Access Journals (Sweden)

    Yucheng eWu

    2015-03-01

    Full Text Available Bacteria and archaea sustain subsurface cave ecosystems by dominating primary production and fueling biogeochemical cyclings, despite the permanent darkness and shortage of nutrients. However, the heterogeneity and underlying mechanism of microbial diversity in caves, in particular those well connect to surface environment are largely unexplored. In this study, we examined the bacterial abundance and composition in Jinjia Cave, a small and shallow limestone cave located on the western Loess Plateau of China, by enumerating and pyrosequencing small subunit (SSU rRNA genes. The results clearly reveal the contrasting bacterial community compositions in relation to cave habitat types, i.e., rock wall deposit, aquatic sediment and sinkhole soil, which are differentially connected to the surface environment. The deposits on the cave walls were dominated by putative cave-specific bacterial lineages within the -Proteobacteria or Actinobacteria that are routinely found on cave rocks around the world. In addition, sequence identity with known functional groups suggests enrichments of chemolithotrophic bacteria potentially involved in autotrophic C fixation and inorganic N transformation on rock surfaces. By contrast, bacterial communities in aquatic sediments were more closely related to those in the overlying soils. This is consistent with the similarity in elemental composition between the cave sediment and the overlying soil, implicating the influence of mineral chemistry on cave microhabitat and bacterial composition. These findings provide compelling molecular evidence of the bacterial community heterogeneity in an East Asian cave, which might be controlled by both subsurface and surface environments.

  15. Some deep caves in Biokovo Mountain (Croatia)

    Science.gov (United States)

    Garasic, Mladen; Garasic, Davor

    2014-05-01

    The investigation of 3 caves explored more than 1000 meters in depth in the Dinaric karst area in Croatia, has been in progress for a considerable period of time. These are complex speleological features situated in the longest mountain range of the Dinaric karst, i.e. at the Northern Velebit mountain range. In fact, these caves have been studied for over two decades now. The first one is a cave system of Lukina jama (Luke's Cave) - Trojama cave, which has been investigated until the depth of 1421 meters (Jalžić, 2007; Šmida, 1993). Its total length is 3731 meters and a new expedition will soon continue to investigate this pit through speleodiving in siphons. The second greatest cave by depth is Slovačka jama (Slovak Cave), 1320 meters in depth, with cave chanals measuring 5677 meters in total length. The third greatest cave by depth is the Cave system of Velebita, reaching down to 1026 m in depth, with the chanal length of 3176 meters (Bakšić, 2006a; 2006b). However, another 3 speleological sites, which can rightly be added to those deeper than 1000 m, have recently been discovered. These are three caverns that were discovered during construction of the Sveti Ilija Tunnel that passes through Mt. Biokovo, in the Dinaric karst area. These caverns undoubtedly point to the link with the ground surface, while the rock overburden above the tunnel in the zone where the caverns were discovered ranges from 1250 and 1350 meters. Bats from the ground surface were found in the caverns and, according to measurements, they are situated in the depth from 200 and 300 meters below the tunnel level. This would mean that the depth of these newly found caves ranges from 1450 and 1650 m, when observed from the ground surface. There are several hundreds of known caves in Biokovo, and the deepest ones discovered so far are Jama Mokre noge (Wet Feet Cave) 831 m in depth, and Jama Amfora (Amphora Cave) 788 m in depth (Bockovac, 1999; Bakšić & all, 2002; Lacković & all, 2001

  16. Mediterranean Recluse Spider, Loxosceles rufescens (Araneae: Sicariidae from Charkhab Cave, Southern Iran

    Directory of Open Access Journals (Sweden)

    Saber Sadeghi

    2017-04-01

    Full Text Available Background: The best-known dangerous spiders belong to the six genera. The genus Loxosceles or violin spiders are well known for their ability to cause skin necrosis or loxoscelism. All Loxosceles species have medical im­portance due to their necrotizing venom. The present article reports the occurrence of L. rufescens in Charkhab Cave, south of Iran (Larestan.Methods: The specimens were collected from the Charkhab Cave using handling forceps, paintbrush and aspirator and preserved in 96% ethanol.Results: Loxosceles rufescens, a medically important spider, is recorded from Charkhab Cave in Fars Province (southwest of Iran. Identification of L. rufescens was performed based on external morphology and the features of male genitalia.Conclusion: Presence of L. rufescens in south of Iran especially in a cave confirmed that this species is a widely distributed species in Iran. Therefore, cavers or cave visitors should be aware of this poisonous spider in caves.

  17. Environmental drivers of phototrophic biofilms in an Alpine show cave (SW-Italian Alps)

    International Nuclear Information System (INIS)

    Piano, E.; Bona, F.; Falasco, E.; La Morgia, V.; Badino, G.; Isaia, M.

    2015-01-01

    The proliferation of lampenflora is a major threat for the conservation of show caves, since phototrophic organisms cause physical, chemical and aesthetic damage to speleothems. In this paper we examine the environmental factors influencing the presence and the growth of the three main photosynthetic groups composing phototrophic biofilms in the Bossea show cave (SW-Italian Alps). The presence and the primary production of cyanobacteria, diatoms and green algae were detected with BenthoTorch®, an instrument for in situ measurement of chlorophyll a concentration that has never been used before in caves. By means of different techniques of regression analysis, we highlighted the response of the three photosynthetic groups to different environmental factors. Illuminance proved to be the main factor influencing positively both the probability of the presence and the productivity of the three groups. The presence of seeping water on the substrate and the distance from the cave entrance proved to play an important role in determining patterns of colonization. By means of GIS techniques, we provide thematic maps of the cave, providing a representation of pattern of the density of the three examined photosynthetic groups within different areas of the cave. The same approach may apply to other show caves, aiming at providing suggestions for the cave management (i.e. cleaning of the cave walls and positioning of artificial lights) and reduce impact caused by tourism. - Highlights: • We used a PAM fluorimeter on autotrophic biofilms in a show cave for the first time. • We modelled the environmental factors influencing phototrophic biofilms. • Illuminance, moisture and distance from the entrance proved to be significant. • We produced thematic maps illustrating our results. • We provide suggestions for cave management

  18. Environmental drivers of phototrophic biofilms in an Alpine show cave (SW-Italian Alps)

    Energy Technology Data Exchange (ETDEWEB)

    Piano, E., E-mail: elena.piano@unito.it [Department of Life Sciences and Systems Biology, University of Turin, Via Accademia Albertina 13, 10123 Turin (Italy); Bona, F.; Falasco, E. [Department of Life Sciences and Systems Biology, University of Turin, Via Accademia Albertina 13, 10123 Turin (Italy); La Morgia, V. [ISPRA, via Ca' Fornacetta, 9, 40064 Ozzano dell' Emilia (Italy); Badino, G. [Department of Physics, University of Turin, Via P. Giuria 1, 10125 Turin (Italy); Isaia, M. [Department of Life Sciences and Systems Biology, University of Turin, Via Accademia Albertina 13, 10123 Turin (Italy)

    2015-12-01

    The proliferation of lampenflora is a major threat for the conservation of show caves, since phototrophic organisms cause physical, chemical and aesthetic damage to speleothems. In this paper we examine the environmental factors influencing the presence and the growth of the three main photosynthetic groups composing phototrophic biofilms in the Bossea show cave (SW-Italian Alps). The presence and the primary production of cyanobacteria, diatoms and green algae were detected with BenthoTorch®, an instrument for in situ measurement of chlorophyll a concentration that has never been used before in caves. By means of different techniques of regression analysis, we highlighted the response of the three photosynthetic groups to different environmental factors. Illuminance proved to be the main factor influencing positively both the probability of the presence and the productivity of the three groups. The presence of seeping water on the substrate and the distance from the cave entrance proved to play an important role in determining patterns of colonization. By means of GIS techniques, we provide thematic maps of the cave, providing a representation of pattern of the density of the three examined photosynthetic groups within different areas of the cave. The same approach may apply to other show caves, aiming at providing suggestions for the cave management (i.e. cleaning of the cave walls and positioning of artificial lights) and reduce impact caused by tourism. - Highlights: • We used a PAM fluorimeter on autotrophic biofilms in a show cave for the first time. • We modelled the environmental factors influencing phototrophic biofilms. • Illuminance, moisture and distance from the entrance proved to be significant. • We produced thematic maps illustrating our results. • We provide suggestions for cave management.

  19. Hurricane Impact on Seepage Water in Larga Cave, Puerto Rico

    Science.gov (United States)

    Vieten, Rolf; Warken, Sophie; Winter, Amos; Schröder-Ritzrau, Andrea; Scholz, Denis; Spötl, Christoph

    2018-03-01

    Hurricane-induced rainfall over Puerto Rico has characteristic δ18O values which are more negative than local rainfall events. Thus, hurricanes may be recorded in speleothems from Larga cave, Puerto Rico, as characteristic oxygen isotope excursions. Samples of 84 local rainfall events between 2012 and 2013 ranged from -6.2 to +0.3‰, whereas nine rainfall samples belonging to a rainband of hurricane Isaac (23-24 August 2012) ranged from -11.8 to -7.1‰. Cave monitoring covered the hurricane season of 2014 and investigated the impact of hurricane rainfall on drip water chemistry. δ18O values were measured in cumulative monthly rainwater samples above the cave. Inside the cave, δ18O values of instantaneous drip water samples were analyzed and drip rates were recorded at six drip sites. Most effective recharge appears to occur during the wet months (April-May and August-November). δ18O values of instantaneous drip water samples ranged from -3.5 to -2.4‰. In April 2014 and April 2015 some drip sites showed more negative δ18O values than the effective rainfall (-2.9‰), implying an influence of hurricane rainfall reaching the cave via stratified seepage flow months to years after the event. Speleothems from these drip sites in Larga cave have a high potential for paleotempestology studies.

  20. Insectivorous bat reproduction and human cave visitation in Cambodia: A perfect conservation storm?

    Science.gov (United States)

    Lim, Thona; Cappelle, Julien; Hoem, Thavry; Furey, Neil

    2018-01-01

    Cave roosting bats represent an important component of Southeast Asian bat diversity and are vulnerable to human disturbance during critical reproductive periods (pregnancy, lactation and weaning). Because dramatic growth of cave tourism in recent decades has raised concerns about impacts on cave bats in the region, we assessed the reproductive phenology of two insectivorous species (Hipposideros larvatus sensu lato and Taphozous melanopogon) at three caves in Cambodia for 23 months in 2014-2016 and evaluated human visitation to these sites between 2007 and 2014. Despite the differing foraging strategies employed by the two taxa, the temporal consistency observed in proportions of pregnant, lactating and juvenile bats indicates that their major birth peaks coincide with the time of greatest cave visitation annually, particularly for domestic visitors and namely during the Cambodian new year in April. They also reflect rainfall patterns and correspond with the reproductive phenology of insectivorous cave bats in Vietnam. These findings were predictable because 1) insect biomass and thus food availability for insectivorous bats are optimal for ensuring survival of young following this period, and 2) the Khmer new year is the most significant month for religious ceremonies and thus domestic cave visitation nationally, due to the abundance of Buddhist shrines and temples in Cambodian caves. While the impact of visitor disturbance on bat population recruitment cannot be empirically assessed due to lack of historical data, it is nonetheless likely to have been considerable and raises a conservation concern. Further, because growing evidence suggests that insectivorous cave bats exhibit reproductive synchrony across continental Southeast Asia where countless cave shrines are heavily frequented during April in Theravada Buddhist countries (e.g., Myanmar, Thailand, Cambodia and Laos), our results may have wider applicability in the region. We consequently advocate for

  1. Insectivorous bat reproduction and human cave visitation in Cambodia: A perfect conservation storm?

    Directory of Open Access Journals (Sweden)

    Thona Lim

    Full Text Available Cave roosting bats represent an important component of Southeast Asian bat diversity and are vulnerable to human disturbance during critical reproductive periods (pregnancy, lactation and weaning. Because dramatic growth of cave tourism in recent decades has raised concerns about impacts on cave bats in the region, we assessed the reproductive phenology of two insectivorous species (Hipposideros larvatus sensu lato and Taphozous melanopogon at three caves in Cambodia for 23 months in 2014-2016 and evaluated human visitation to these sites between 2007 and 2014. Despite the differing foraging strategies employed by the two taxa, the temporal consistency observed in proportions of pregnant, lactating and juvenile bats indicates that their major birth peaks coincide with the time of greatest cave visitation annually, particularly for domestic visitors and namely during the Cambodian new year in April. They also reflect rainfall patterns and correspond with the reproductive phenology of insectivorous cave bats in Vietnam. These findings were predictable because 1 insect biomass and thus food availability for insectivorous bats are optimal for ensuring survival of young following this period, and 2 the Khmer new year is the most significant month for religious ceremonies and thus domestic cave visitation nationally, due to the abundance of Buddhist shrines and temples in Cambodian caves. While the impact of visitor disturbance on bat population recruitment cannot be empirically assessed due to lack of historical data, it is nonetheless likely to have been considerable and raises a conservation concern. Further, because growing evidence suggests that insectivorous cave bats exhibit reproductive synchrony across continental Southeast Asia where countless cave shrines are heavily frequented during April in Theravada Buddhist countries (e.g., Myanmar, Thailand, Cambodia and Laos, our results may have wider applicability in the region. We consequently

  2. Insectivorous bat reproduction and human cave visitation in Cambodia: A perfect conservation storm?

    Science.gov (United States)

    Cappelle, Julien; Hoem, Thavry

    2018-01-01

    Cave roosting bats represent an important component of Southeast Asian bat diversity and are vulnerable to human disturbance during critical reproductive periods (pregnancy, lactation and weaning). Because dramatic growth of cave tourism in recent decades has raised concerns about impacts on cave bats in the region, we assessed the reproductive phenology of two insectivorous species (Hipposideros larvatus sensu lato and Taphozous melanopogon) at three caves in Cambodia for 23 months in 2014–2016 and evaluated human visitation to these sites between 2007 and 2014. Despite the differing foraging strategies employed by the two taxa, the temporal consistency observed in proportions of pregnant, lactating and juvenile bats indicates that their major birth peaks coincide with the time of greatest cave visitation annually, particularly for domestic visitors and namely during the Cambodian new year in April. They also reflect rainfall patterns and correspond with the reproductive phenology of insectivorous cave bats in Vietnam. These findings were predictable because 1) insect biomass and thus food availability for insectivorous bats are optimal for ensuring survival of young following this period, and 2) the Khmer new year is the most significant month for religious ceremonies and thus domestic cave visitation nationally, due to the abundance of Buddhist shrines and temples in Cambodian caves. While the impact of visitor disturbance on bat population recruitment cannot be empirically assessed due to lack of historical data, it is nonetheless likely to have been considerable and raises a conservation concern. Further, because growing evidence suggests that insectivorous cave bats exhibit reproductive synchrony across continental Southeast Asia where countless cave shrines are heavily frequented during April in Theravada Buddhist countries (e.g., Myanmar, Thailand, Cambodia and Laos), our results may have wider applicability in the region. We consequently advocate for

  3. A conservation status index, as an auxiliary tool for the management of cave environments

    Directory of Open Access Journals (Sweden)

    Christiane Ramos Donato

    2014-09-01

    Full Text Available The conservation of the Speleological Heritage involves bioecological, geomorphological and anthropogenic studies, both from inside the caves and from the external environments that surround them. This study presents a method to rank caves according to their priority for conservation and restoration. Nine caves were evaluated: indicators related to the environmental impacts and the vulnerability status presented by those caves (intrinsic features and the values scored in a ‘Cave Conservation Index’ (CCI were established. We also used a rapid assessment protocol to measure cave vulnerability for prioritization of conservation/restoration actions (RAP-cr comparing natural cavities with the same lithology, due to “strictu sensu” peculiarities. Based on the protocols applied in caves of the municipality of Laranjeiras, Sergipe, Northeastern Brazil, we concluded that the present method attended to the needs for the classification of the caves into categories of conservation/restoration status, using little time and financial effort, through rapid diagnostics that facilitate the comparisons. In this perspective, the CCI can be used to indicate areas that should be protected and caves that should be prioritized to have initiated activities of conservation and restoration.

  4. U-series dating of Paleolithic art in 11 caves in Spain.

    Science.gov (United States)

    Pike, A W G; Hoffmann, D L; García-Diez, M; Pettitt, P B; Alcolea, J; De Balbín, R; González-Sainz, C; de las Heras, C; Lasheras, J A; Montes, R; Zilhão, J

    2012-06-15

    Paleolithic cave art is an exceptional archive of early human symbolic behavior, but because obtaining reliable dates has been difficult, its chronology is still poorly understood after more than a century of study. We present uranium-series disequilibrium dates of calcite deposits overlying or underlying art found in 11 caves, including the United Nations Educational, Scientific, and Cultural Organization (UNESCO) World Heritage sites of Altamira, El Castillo, and Tito Bustillo, Spain. The results demonstrate that the tradition of decorating caves extends back at least to the Early Aurignacian period, with minimum ages of 40.8 thousand years for a red disk, 37.3 thousand years for a hand stencil, and 35.6 thousand years for a claviform-like symbol. These minimum ages reveal either that cave art was a part of the cultural repertoire of the first anatomically modern humans in Europe or that perhaps Neandertals also engaged in painting caves.

  5. Ancient photosynthetic eukaryote biofilms in an Atacama Desert coastal cave

    Science.gov (United States)

    Azua-Bustos, A.; Gonzalez-Silva, C.; Mancilla, R.A.; Salas, L.; Palma, R.E.; Wynne, J.J.; McKay, C.P.; Vicuna, R.

    2009-01-01

    Caves offer a stable and protected environment from harsh and changing outside prevailing conditions. Hence, they represent an interesting habitat for studying life in extreme environments. Here, we report the presence of a member of the ancient eukaryote red algae Cyanidium group in a coastal cave of the hyperarid Atacama Desert. This microorganism was found to form a seemingly monospecific biofilm growing under extremely low photon flux levels. Our work suggests that this species, Cyanidium sp. Atacama, is a new member of a recently proposed novel monophyletic lineage of mesophilic "cave" Cyanidium sp., distinct from the remaining three other lineages which are all thermo-acidophilic. The cave described in this work may represent an evolutionary island for life in the midst of the Atacama Desert. ?? Springer Science + Business Media, LLC 2009.

  6. Simultaneous caving and surface restoration system for oil shale mining

    Energy Technology Data Exchange (ETDEWEB)

    Allsman, P.T.

    1968-10-01

    A modified caving method is introduced for mining oil shale and simultaneous restoration of the land surface by return of spent shale onto the subsided area. Other methods have been designed to mine the relatively thin richer beds occurring near outcrops in the Piceance Creek Basin of NW. Colorado. Since the discovery of the much thicker beds in the N.-central part of the basin, some attention has focused on in situ and open-pit methods of recovery. Although caving has been recognized as a possible means of mining shale, most people have been skeptical of its success. This stems from the unknown and salient factors of cavability and size of broken rock with caving. Wisdom would seem to dictate that serious evaluation of the caving method be made along with the other methods.

  7. Near Surface Geophysical Methods Applied to the Rising Star Cave System

    Science.gov (United States)

    Webb, S. J.; Naidoo, M.; Elliott, M. C.; Kruger, A.; Roberts, E.; Dirks, P.

    2017-12-01

    The Rising Star Cave system is located approximately 40 km northwest of Johannesburg in the Malmani dolomites (Chuniespoort group,Transvaal Supergroup). The cave system is extensive with 4 km of mapped passages and chambers. The Dinaledi chamber, host to the Homo Naledi fossils, is reached by following a tortuous route with squeezes as small as 20 cm. The chamber is located 30 m below surface and 80 m from the entrance. The enigmatic find of fossils from at least 15 individual hominins, without the presence of other species, led to the idea of deliberate burial. The present access route is difficult and it is unclear how early hominins were able to navigate it, prompting the suggestion of an undiscovered entrance. We are using near surface geophysical methods to investigate possible connections between the surface and the caves. Using a Geometrics Cs-vapor Walkmag, we collected preliminary ground magnetic intensity measurements over a region 300 m x 200 m, using 1 m station spacing and 10 m line spacing. The average magnetic variation along line is 200 nT. We also collected over 100 susceptibility measurements on outcropping lithologies, surface soil and cave sediments using a SM-30 susceptibility meter. The surface soil was one to two orders of magnitude higher than surrounding lithologies (average = 1.5 x 10-3 SI) and the cave sediment samples were slightly higher (average = 3.07 x 10-3 SI). We were able to collect GPR data (GSSI SIR-3000, 400 MHz) in selected spots on the cave floor with the goal of locating the cave floor beneath the sediments. Dolomites usually have low magnetic susceptibilities, but erosion products of the nearby magnetic Hospital Hill or Rooihoogte shales may have been transported into or onto the cave system. This is a likely cause of the magnetic anomalies and larger amplitude anomalies may indicate an accumulation of sediments, extending to depth. These anomalies will be further investigated using gravity to determine if there are

  8. NEW LOCALITY OF CAVE BEAR (Ursus spelaeus Rosenmüller & Heinroth, 1794 IN BOSNIA AND HERZEGOVINA: MORPHO- ANATOMICAL CHARACTERISTICS OF CRANIAL SKELETON FOUND IN CAVE AT VRELO MOKRANJSKA MILJACKA

    Directory of Open Access Journals (Sweden)

    Lada Lukić-Bilela

    2013-10-01

    Full Text Available During the investigation of the Cave at Vrelo Mokranjska Miljacka, the bone remains of the cave bear (Ursus spelaeus Rosenmüller & Heinroth, 1794 was found. This is the new Pleistocene fauna locality of this extinct species in Bosnia and Herzegovina. Almost complete cranial skeleton belongs to a young adult male. Analyzed morphometric proportions completely fit within the variation range of the Pleistocene cave bear populations in Bosnia and Herzegovina.Key words: Ursus spelaeus, skull, teeth, Cave of Vrelo Mokranjska Miljacka

  9. Investigation of tracking systems properties in CAVE-type virtual reality systems

    Science.gov (United States)

    Szymaniak, Magda; Mazikowski, Adam; Meironke, Michał

    2017-08-01

    In recent years, many scientific and industrial centers in the world developed a virtual reality systems or laboratories. One of the most advanced solutions are Immersive 3D Visualization Lab (I3DVL), a CAVE-type (Cave Automatic Virtual Environment) laboratory. It contains two CAVE-type installations: six-screen installation arranged in a form of a cube, and four-screen installation, a simplified version of the previous one. The user feeling of "immersion" and interaction with virtual world depend on many factors, in particular on the accuracy of the tracking system of the user. In this paper properties of the tracking systems applied in I3DVL was investigated. For analysis two parameters were selected: the accuracy of the tracking system and the range of detection of markers by the tracking system in space of the CAVE. Measurements of system accuracy were performed for six-screen installation, equipped with four tracking cameras for three axes: X, Y, Z. Rotation around the Y axis was also analyzed. Measured tracking system shows good linear and rotating accuracy. The biggest issue was the range of the monitoring of markers inside the CAVE. It turned out, that the tracking system lose sight of the markers in the corners of the installation. For comparison, for a simplified version of CAVE (four-screen installation), equipped with eight tracking cameras, this problem was not occur. Obtained results will allow for improvement of cave quality.

  10. Nutrição mineral da fava d'anta Mineral nutrition of Dimorphandra mollis

    Directory of Open Access Journals (Sweden)

    Candido Alves da Costa

    2007-03-01

    Full Text Available Com o objetivo de caracterizar as limitações nutricionais na produção, no crescimento, nutrição e teores de flavonóides totais em fava-d'anta, um experimento foi conduzido em casa de vegetação com amostras de 0-20 cm de profundidade de um Latossolo Vermelho sob vegetação de cerrado. O delineamento experimental utilizado foi o inteiramente casualizado com 13 tratamentos e três repetições. Os tratamentos constaram da adubação com elemento faltante (omitindo-se a calagem e cada um dos nutrientes N, P, K, S, B, Cu, Fe e Zn. Verificou-se que a fava d'anta é tolerante à acidez do solo e sensível à deficiência de fósforo e potássio. O teor de flavonóides totais não variou entre os tratamentos.Aiming to characterize the nutritional limitations on growth, nutrition and total flavonoids concentration of the Dimorphandra mollis, an experiment was conducted in a greenhouse with 0-20 cm samples of an Oxisol under Cerrado vegetation. The experimental design was completely randomized with 13 treatments and three replications. The treatments consisted of fertilization with missing element technique (with omission of liming and each one of the macro and micronutrients N, P, K, S, B, Cu, Fe and Zn. Dimorphandra mollis plant is tolerant to acid soil and responsive to the omission of phosphorus and potassium. The total flavonoids concentration was not influenced by the treatments.

  11. Ancient DNA reveals differences in behaviour and sociality between brown bears and extinct cave bears.

    Science.gov (United States)

    Fortes, Gloria G; Grandal-d'Anglade, Aurora; Kolbe, Ben; Fernandes, Daniel; Meleg, Ioana N; García-Vázquez, Ana; Pinto-Llona, Ana C; Constantin, Silviu; de Torres, Trino J; Ortiz, Jose E; Frischauf, Christine; Rabeder, Gernot; Hofreiter, Michael; Barlow, Axel

    2016-10-01

    Ancient DNA studies have revolutionized the study of extinct species and populations, providing insights on phylogeny, phylogeography, admixture and demographic history. However, inferences on behaviour and sociality have been far less frequent. Here, we investigate the complete mitochondrial genomes of extinct Late Pleistocene cave bears and middle Holocene brown bears that each inhabited multiple geographically proximate caves in northern Spain. In cave bears, we find that, although most caves were occupied simultaneously, each cave almost exclusively contains a unique lineage of closely related haplotypes. This remarkable pattern suggests extreme fidelity to their birth site in cave bears, best described as homing behaviour, and that cave bears formed stable maternal social groups at least for hibernation. In contrast, brown bears do not show any strong association of mitochondrial lineage and cave, suggesting that these two closely related species differed in aspects of their behaviour and sociality. This difference is likely to have contributed to cave bear extinction, which occurred at a time in which competition for caves between bears and humans was likely intense and the ability to rapidly colonize new hibernation sites would have been crucial for the survival of a species so dependent on caves for hibernation as cave bears. Our study demonstrates the potential of ancient DNA to uncover patterns of behaviour and sociality in ancient species and populations, even those that went extinct many tens of thousands of years ago. © 2016 John Wiley & Sons Ltd.

  12. Radon and thoron in cave dwellings (Yan'an, China)

    International Nuclear Information System (INIS)

    Wiegand, J.; Feige, S.; Xie Quingling; Schreiber, U.; Wieditz, K.; Wittmann, C.; Luo Xiarong

    2000-01-01

    222 Rn and 220 Rn concentrations were measured in cave dwellings and brick houses in the region of Yan'an (China) during summer 1997. The underground dwellings are built into Quaternary loess, and all investigated houses are founded on it. The median values of indoor 222 Rn and 220 Rn concentrations are 42 (n = 18) and 77Bq m -3 (n = 15) for brick houses and 92 (n = 23) and 215 (n = 17) Bq m -3 for cave dwellings. To classify the dwellings in respect to their cave-character, the fraction of walls having a direct contact to the loess is calculated for each dwelling. While the 222 Rn concentrations are increasing with higher fractions, the 220 Rn concentrations are not correlated with this fraction. On the other hand, due to the short half-life of 220 Rn the distance from the measuring point to the walls is negatively correlated with the 220 Rn concentration, while there is no correlation with the 222 Rn concentration. Therefore, concentric isolines of 220 Rn concentrations showing a strong gradient were detected in cave dwellings. An influence of the ventilation rate is distinct for 222 Rn but weak for 220 Rn. The effective dose rates for 222 Rn and 220 Rn and their progenies are calculated for brick houses (2.7 mSv y -1 ), cave dwellings (7.1 mSv y -1 ), and for traditional cave dwellings with a bed foundation built with loess (16.7 mSv y -1 ). These calculations are based on summer measurements only. It is expected that the true effective dose rates will be significantly higher

  13. Measurements of seasonal and daily radon daughter concentration fluctuations in National Park Service caves

    International Nuclear Information System (INIS)

    Yarborough, K.A.

    1977-01-01

    The National Park Service (NPS) is studying levels of airborne alpha radiation from radon and thoron in all NPS administered caves in which tours for visitors are regularly conducted. The NPS research has the dual but complementary objectives of safeguarding health at the NPS administered caves, and to develop data on alpha radiation levels and on natural airflows in NPS caves. The results reported here for NPS caves describe concepts hypothesized for the objectives. In addition the data can be used by various agencies to clarify health standards for exposures to low airborne alpha radiation levels in cave environments. These results show daily and seasonal trends and the influence of natural ventilation by air circulation for each cave investigated

  14. Cure from the cave: volcanic cave actinomycetes and their potential in drug discovery

    Directory of Open Access Journals (Sweden)

    Cheeptham N.

    2013-01-01

    Full Text Available Volcanic caves have been little studied for their potential as sources of novel microbial species and bioactive compounds with new scaffolds. We present the f irst study of volcanic cave microbiology from Canada and suggest that this habitat has great potential for the isolation of novel bioactive substances. Sample locat ions were plot ted on a contour map that was compiled in ArcView 3.2. Over 400 bacterial isolates were obtained from the Helmcken Falls cave in Wells Gray Provincial Park, British Columbia. From our preliminary screen, of 400 isolates tested, 1% showed activity against extended spectrum ß-lactamase E. coli, 1.75% against Escherichia coli, 2.25% against Acinetobacter baumannii, and 26.50% against Klebsiella pneumoniae. In addition, 10.25% showed activity against Micrococcus luteus, 2% against methicillin resistant Staphylococcus aureus, 9.25% against Mycobacterium smegmatis, 6.25% Pseudomonas aeruginosa and 7.5% against Candida albicans. Chemical and physical characteristics of three rock wall samples were studied using scanning electron microscopy and f lame atomic absorption spectrometry. Calcium (Ca, iron (Fe, and aluminum (Al were the most abundant components while magnesium (Mg, sodium (Na, arsenic (As, lead (Pb, chromium (Cr, and barium (Ba were second most abundant with cadmium (Cd and potassium (K were the least abundant in our samples. Scanning electron microscopy (SEM showed the presence of microscopic life forms in all three rock wall samples. 16S rRNA gene sequencing of 82 isolates revealed that 65 (79.3% of the strains belong to the Streptomyces genus and 5 (6.1% were members of Bacillus, Pseudomonas, Nocardia and Erwinia genera. Interestingly, twelve (14.6% of the 16S rRNA sequences showed similarity to unidentif ied ribosomal RNA sequences in the library databases, the sequences of these isolates need to be further investigated using the EzTaxon-e database (http://eztaxon-e. ezbiocloud.net/ to determine whether

  15. An absolutely dated high-resolution stalagmite record from Lianhua Cave in central China: Climate forcing and comparison with Wanxiang Cave and Dongge Cave records over the past 2000 years

    Science.gov (United States)

    Li, Hong-Chun; Yin, Jian-Jun; Shen, Chuan-Chou; Mii, Horng-Sheng; Li, Ting-Yong

    2015-04-01

    A 33-cm long aragonite stalagmite (LHD-1) from Lianhua Cave has been dated by MC-ICPMS 230Th/U method on 41 horizons. Very high U contents (1~6ppm) and low Th contents yield excellent 230Th/U dates which provide reliable chronology of the stalagmite on sub-decadal time scale over the past 3350 years. A total of 1716 samples have been measured for δ18O and δ13C, spanning annual resolution over the past 1820 years. The stalagmite δ18O is not only influenced by the 'amount effect', but also affected by the moisture source. Enhanced the tropical monsoon trough under strong EASM brings higher spring quarter rainfall with isotopically light monsoonal moisture in the cave site, resulting in lighter stalagmite δ18O. On decadal or longer time scales, increased solar activity produces warmer condition and stronger summer monsoon which lead to wet climates. On interannual-to-decadal scales, the Walker Circulation under El Niño conditions during cold periods will shift toward the central Pacific and result in weakening of EASM. Under such a circumstance, dry climates will be prevailed in the study area. Based on the δ18O and δ13C records, we have deciphered climatic and vegetation changes of the study area in decadal scales. The highly precise dated LHD-1 record has been compared with previous published Wanxiang Cave and Dongge Cave records. Although some similarities can be found, there are major discrepancies among the three well-dated records, especially during AD 500-700 and AD 1300-1600. In additional, the major weak monsoon periods defined in the Wanxiang Cave record during late Tang Dynasty, late Yuan Dynasty and late Ming Dynasty are not supported by the LHD-1 record. The heaviest δ18O peaks (more than five continuous heavy values) over the past 2000 years appeared around AD 1990-2003, 1657-1662, 1220-1228, 663-669, 363-370, and 1082-1090 (in the order of heavy to light). None of these periods occurred Chinese dynasty collapse.

  16. Constituintes químicos das folhas e do caule de Coccoloba mollisCasaretto (Polygonaceae)

    OpenAIRE

    Oliveira,Patrícia Emanuella S.; Santos,Wagner S. dos; Conserva,Lucia M.; Lemos,Rosangela P. de Lyra

    2008-01-01

    O estudo químico das frações neutra em hexano das folhas e em diclorometano do caule de Coccoloba mollis resultou no isolamento de um triterpeno pentacíclico (simiarenol), que pela primeira vez ocorre em Polygonaceae, dois fitoesteróides (sitostenona e sitosterol), um diterpeno (trans-fitol) e de um benzenóide (ácido vanílico) que está sendo descrito pela vez neste gênero. A identificação estrutural destes compostos foi feita com base na análise dos dados espectrais (IV, EM e RMN, incluindo D...

  17. Nitrite toxicity assessment in Danio rerio and Poecilia reticulata

    Directory of Open Access Journals (Sweden)

    Petra Doleželová

    2011-01-01

    Full Text Available Nitrite is a natural component of the nitrogen cycle in the environment. Although it usually occurs in low concentrations, elevated concentrations caused by effluents or affected nitrification process can lead to serious health deterioration of fish. Two aquarium fish zebrafish (Danio rerio and guppy (Poecilia reticulata are recommended to use as model organisms in toxicity tests. However, their sensitivity to nitrite can differ. The aim of this study was to define acute toxicity of nitrite by the semistatic method according to OECD No. 203 (Fish, Acute toxicity test. The series of 4 acute toxicity tests was performed, with 10 fish of both species used for each concentration and for the control. The 96hLC50 NO2- value for D. rerio and P. reticulata was 242.55 ± 15.79 mg·l-1 and 30.2 ± 8.74 mg·l-1, respectively. We have proved significant difference (p D. rerio and P. reticulata. The results showed different sensitivities to nitrites in tested fish species, which could be related to species-specific branchial chloride uptake mechanism. This is the first study on this fish species.

  18. Observations on the Cave-Associated Beetles (Coleoptera of Nova Scotia, Canada

    Directory of Open Access Journals (Sweden)

    Moseley M.

    2009-07-01

    Full Text Available The cave-associated invertebrates of Nova Scotia constitute a fauna at a very early stage of post-glacial recolonization. TheColeoptera are characterized by low species diversity. A staphylinid Quedius spelaeus spelaeus, a predator, is the only regularlyencountered beetle. Ten other terrestrial species registered from cave environments in the province are collected infrequently. Theyinclude three other rove-beetles: Brathinus nitidus, Gennadota canadensis and Atheta annexa. The latter two together with Catopsgratiosus (Leiodidae constitute a small group of cave-associated beetles found in decompositional situations. Quedius s. spelaeusand a small suite of other guanophiles live in accumulations of porcupine dung: Agolinus leopardus (Scarabaeidae, Corticariaserrata (Latrididae, and Acrotrichis castanea (Ptilidae. Two adventive weevils Otiorhynchus ligneus and Barypeithes pellucidus(Curculionidae collected in shallow cave passages are seasonal transients; Dermestes lardarius (Dermestidae, recorded fromone cave, was probably an accidental (stray. Five of the terrestrial beetles are adventive Palaearctic species. Aquatic beetles arecollected infrequently. Four taxa have been recorded: Agabus larsoni (Dytiscidae may be habitual in regional caves; another Agabussp. (probably semivittatus, Dytiscus sp. (Dytiscidae, and Crenitis digesta (Hydrophilidae are accidentals. The distribution andecology of recorded species are discussed, and attention is drawn to the association of beetles found in a Nova Scotia “ice cave”.

  19. Studies of condensation/evaporation processes in the Glowworm Cave, New Zealand

    Directory of Open Access Journals (Sweden)

    de Freitas Chris R.

    2006-07-01

    Full Text Available The condensation/evaporation process is important in caves, especially in tourist caves where there is carbon dioxide enriched air caused by visitors. The cycle of condensation and evaporation of condensate is believed to enhance condensation corrosion. The problem is condensation is difficult to measure. This study addresses the problem and reports on a method for measuring and modelling condensation rates in a limestone cave. Electronic sensors for measuring condensation and evaporation of the condensate as part of a single continuous process of water vapour flux are tested and used to collect 12 months of data. The study site is the Glowworm tourist cave in New Zealand. The work describes an explanatory model of processes leading to condensation using data based on measurements of condensation and evaporation as part of a single continuous process of water vapour flux. The results show that the model works well. However, one of the most important messages from the research reported here is the introduction of the condensation sensor. The results show that condensation in caves can actually be measured and monitored, virtually in real time. In conjunction with the recent developments in data logging equipment, this opens exciting perspectives in cave climate studies, and, more generally, in hydrogeological studies in karst terrains.

  20. Riipaisevia melodioita ja tulista taiturointia : Karakteerierot Max Bruchin Viulukonsertossa nro 1 g-molli Op. 26

    OpenAIRE

    Kuusirati, Veera

    2017-01-01

    Opinnäytetyössäni tarkastelen Max Bruchin Viulukonserttoa nro 1 g-molli op. 26 ja sen tul-kitsemista ja harjoittelua viulistin näkökulmasta. Syvennyn erityisesti pohtimaan, miten kon-serttoon sisältyvät karakteerierot saisi mahdollisimman hyvin kuulumaan soitossa. Lisäksi kerron Bruchin elämästä ja urasta säveltäjänä. Tarkastelen konserttoa analysoimalla teosta tyylillisesti ja rakenteellisesti ja partituuria tut-kimalla. Lisäksi olen tutkinut teosta soittamalla ja harjoittelemalla sitä. ...

  1. Chemical Ecology of Cave-Dwelling Millipedes: Defensive Secretions of the Typhloiulini (Diplopoda, Julida, Julidae).

    Science.gov (United States)

    Makarov, Slobodan E; Bodner, Michaela; Reineke, Doris; Vujisić, Ljubodrag V; Todosijević, Marina M; Antić, Dragan Ž; Vagalinski, Boyan; Lučić, Luka R; Mitić, Bojan M; Mitov, Plamen; Anđelković, Boban D; Lucić, Sofija Pavković; Vajs, Vlatka; Tomić, Vladimir T; Raspotnig, Günther

    2017-04-01

    Cave animals live under highly constant ecological conditions and in permanent darkness, and many evolutionary adaptations of cave-dwellers have been triggered by their specific environment. A similar "cave effect" leading to pronounced chemical interactions under such conditions may be assumed, but the chemoecology of troglobionts is mostly unknown. We investigated the defensive chemistry of a largely cave-dwelling julid group, the controversial tribe "Typhloiulini", and we included some cave-dwelling and some endogean representatives. While chemical defense in juliform diplopods is known to be highly uniform, and mainly based on methyl- and methoxy-substituted benzoquinones, the defensive secretions of typhloiulines contained ethyl-benzoquinones and related compounds. Interestingly, ethyl-benzoquinones were found in some, but not all cave-dwelling typhloiulines, and some non-cave dwellers also contained these compounds. On the other hand, ethyl-benzoquinones were not detected in troglobiont nor in endogean typhloiuline outgroups. In order to explain the taxonomic pattern of ethyl-benzoquinone occurrence, and to unravel whether a cave-effect triggered ethyl-benzoquinone evolution, we classed the "Typhloiulini" investigated here within a phylogenetic framework of julid taxa, and traced the evolutionary history of ethyl-benzoquinones in typhloiulines in relation to cave-dwelling. The results indicated a cave-independent evolution of ethyl-substituted benzoquinones, indicating the absence of a "cave effect" on the secretions of troglobiont Typhloiulini. Ethyl-benzoquinones probably evolved early in an epi- or endogean ancestor of a clade including several, but not all Typhloiulus (basically comprising a taxonomic entity known as "Typhloiulus sensu stricto") and Serboiulus. Ethyl-benzoquinones are proposed as novel and valuable chemical characters for julid systematics.

  2. Main karst and caves of Switzerland; El karst y las cuevas mas importantes de Suiza

    Energy Technology Data Exchange (ETDEWEB)

    Jeannin, P. Y.

    2016-07-01

    This paper presents an overview of the main karst areas and cave systems in Switzerland. The first part encloses descriptions of the main geological units that hold karst and caves in the country and summarizes a brief history of research and protection of the cave environments. The second part presents three regions enclosing large cave systems. Two regions in the Alps enclose some of the largest limestone caves in Europe: Siebenhengste (Siebenhengste cave system with ∼160 km and Barenschacht with 70 km) and Bodmeren-Silberen (Holloch cave system with 200 km and Silberen System with 39 km). These systems are also among the deepest with depths ranging between 880 and 1340 m. The third example is from the Jura Mountains (northern Switzerland). (Author)

  3. Radiocarbon intercomparison program for Chauvet Cave

    International Nuclear Information System (INIS)

    Cuzange, M.T.; Delque-Kolic, E.; Oberlin, C.; Goslar, T.; Grootes, P.M.; Nadeau, M.J.; Higham, T.; Ramsey, C.B.; Kaltnecker, E.; Paterne, M.; Valladas, H.; Van der Plicht, J.; Van der Plicht, J.; Clottes, J.; Geneste, J.M.

    2007-01-01

    We present the first results of an accelerator mass spectrometry (AMS) radiocarbon intercomparison program on 3 different charcoal samples collected in one of the hearths of the Megaceros gallery of Chauvet Cave (Ardeche, France). This cave, rich in parietal decoration, is important for the study of the appearance and evolution of prehistoric art because certain drawings have been 14 C dated to the Aurignacian period at the beginning of the Upper Paleolithic. The new dates indicate an age of about 32,000 BP, which is consistent with this attribution and in agreement with the results from the same sector of the cave measured previously at the Laboratoire des Sciences du Climat et de l'Environnement (LSCE). Six laboratories were involved in the intercomparison. Samples were measured in 4 AMS facilities: Center for Isotope Research, Groningen University, the Netherlands; the Oxford Radiocarbon Accelerator Unit, UK; the Centre de datation par le carbone 14, Univ. Claude Bernard Lyon 1, France (measured by AMS facilities of Poznan University, Poland); and the LSCE, UMR CEA-CNRS-UVSQ, France (measured by the Leibniz-Labor of Christian-Albrechts-Universitat Kiel, Germany). (authors)

  4. Characterization and management trends of negative and positive impacts of tourism in show caves

    Directory of Open Access Journals (Sweden)

    Heros Augusto Santos Lobo

    2015-08-01

    Full Text Available Caves are one of the most fragile environments on Earth ’s surface, in function of physical, atmospheric and biological specificities. The natural characteristics associated with cultural aspects of local people are the reasons to tourist potential of caves. However, the tourist use of caves can generate negative impacts on the environment. This consequence happens when inappropriate procedures of planning and management of tourism are used. Negative impacts are described in this review, as also indications of management techniques to avoid, decrease or eliminate the negative consequences of tourism in caves. Positive impacts are also presented, in order to achieve a sustainable tourism in show caves. The conclusion argues that the negative impacts should not be considered as impediments to tourism in caves. It is important to know these impacts and use it as a key to get previous answers which allow to raise the sustainability of show caves.

  5. Pisgah Lava Cave Communication Test: Science Case Study for the Networked Constellations Initiative

    Science.gov (United States)

    Belov, K.; Ellison, D.; Fraeman, A.

    2017-01-01

    As part of the science case study for the Networked Constellations initiative, a team of JPL scientists explore the possibility of a mission to study the lava caves on Mars. Natural caves on Mars and the Moon present a unique opportunity to learn about the planetary geology and to provide a shelter for human explorers. Due to power and communication challenges, a network of assets has significant advantages over a single asset sent inside a cave. However, communication between the assets and the data downlink present significant difficulties due to the presence of rough walls, boulders, and other obstacles with unknown dielectric constant inside a typical cave, disturbing the propagation of the radio waves. A detailed study is needed to establish the limitations of the current communication technologies and to develop requirements for the new communication technology applicable to the cave environment. On May 4 of 2017, Konstantin Belov, Doug Ellison, and Abby Fraeman visited a lava cave in Pisgah, CA. The purpose of the visit was to build a 3D map of the cave, which could be used to create a model of radio wave propagation, and to conduct a series of communication tests using off-the-shelf equipment to verify the in-cave communication challenges. This experiment should be considered as a simple 'proof of concept' and is the subject of this report.

  6. Dating Petroglyphs from Fugoppe Cave, Japan

    Directory of Open Access Journals (Sweden)

    Masaru Ogawa

    2014-01-01

    Full Text Available For over 20 years, I have tried to establish a relative date for petroglyphs in Fugoppe Cave, Japan. Unsuspected amidst debris accumulating from about 1300 years ago, the petroglyphs were rediscovered accidentally in 1950. From an analysis of petroglyphs on fallen rocks scattered randomly on the site floor, I argue that the artworks date from ca.1900 years ago. The cave itself, formed by wave action, saw its main occupation by pottery-making people from 1700–1500 years ago; although the petroglyphs on the rock walls predated their occupation, it seems unlikely that the occupants attached any meaning to them.

  7. Unexpected diversity of sandflies (Diptera: Psychodidae) in tourist caves in Northern Thailand.

    Science.gov (United States)

    Sukantamala, Jedsada; Sing, Kong-Wah; Jaturas, Narong; Polseela, Raxsina; Wilson, John-James

    2017-11-01

    Certain species of Phlebotomine sandflies (Diptera: Psychodidae) are vectors of the protozoa which causes leishmaniasis. Sandflies are found breeding in enclosed places like caves. Thailand is a popular tourist destination, including for ecotourism activities like caving, which increases the risk of contact between tourists and sandflies. Surveillance of sandflies is important for monitoring this risk but identification of species based on morphology is challenged by phenotypic plasticity and cryptic diversity. DNA barcodes have been used for the identification of sandflies in Thailand. We collected sandflies using CDC light trap from four tourist caves in Northern Thailand. Female sandflies were provisionally sorted into 13 morphospecies and 19 unidentified specimens. DNA was extracted from the thorax and legs of sandflies and the DNA barcode region of cytochrome c oxidase I mtDNA amplified and sequenced. The specimens were sorted into 22 molecular operational taxonomic units (MOTU) based on the 145 DNA barcodes, which is significantly more than the morphospecies. Several of the taxa thought to be present in multiple caves, based on morphospecies sorting, split into cave-specific MOTU which likely represent cryptic species. Several MOTU reported in an earlier study from Wihan Cave, Thailand, were also found in these caves. This supports the use of DNA barcodes to investigate species diversity of sandflies and their useful role in surveillance of sandflies in Thailand.

  8. Cave-dwelling bats (Mammalia: Chiroptera) and conservation concerns in South central Mindanao, Philippines

    OpenAIRE

    Krizler C. Tanalgo; John Aries G. Tabora

    2015-01-01

    The stable microclimate in caves provides a relatively constant habitat for many bat species in the Philippines, but human encroachment continues to disrupt this habitat and imperil many of the species roosting in the caves.  In South central Mindanao, the diversity and conservation status of cave bats remain undocumented and unexplored.  We employed mist-netting to capture bats from five different caves within the town of Kabacan, northern Cotabato, Philippines.  A total of 14 bat species we...

  9. Cave Holography – Out of the lab and under the ground

    International Nuclear Information System (INIS)

    Klayer, J

    2013-01-01

    This paper describes the combination of my hobbies, caving and holography. Most traditional holography involves bringing the objects to a lab with all the necessary holography equipment mounted on a stable table. I instead bring all the equipment assembled as a portable unit to the natural formations in a cave with the cave itself being the stable table. The first successes were Denisyuks made with a HeNe or laser diode and spatial filter mounted on a tripod. For greater depth, transmission holograms were made with a DPSS laser in several configurations sometimes using fiber optics to route the reference beam and sometimes a spatial filter and mirrors. The cave environment presents unique obstacles that have been overcome as evidenced by the beautiful holograms made.

  10. Hominin-bearing caves and landscape dynamics in the Cradle of Humankind, South Africa

    Science.gov (United States)

    Dirks, Paul H. G. M.; Berger, Lee R.

    2013-02-01

    This paper provides constraints on the evolution of the landscape in the Cradle of Humankind (CoH), UNESCO World Heritage Site, South Africa, since the Pliocene. The aim is to better understand the distribution of hominin fossils in the CoH, and determine links between tectonic processes controlling the landscape and the evolution and distribution of hominins occupying that landscape. The paper is focused on a detailed reconstruction of the landscape through time in the Grootvleispruit catchment, which contains the highly significant fossil site of Malapa and the remains of the hominin species Australopithicus sediba. In the past 4 My the landscape in the CoH has undergone major changes in its physical appearance as a result of river incision, which degraded older African planation surfaces, and accommodated denudation of cover rocks (including Karoo sediments and various sil- and ferricretes) to expose dolomite with caves in which fossils collected. Differentially weathered chert breccia dykes, calibrated with 10Be exposure ages, are used to estimate erosion patterns of the landscape across the CoH. In this manner it is shown that 2 My ago Malapa cave was ˜50 m deep, and Gladysvale cave was first exposed; i.e. landscape reconstructions can provide estimates for the time of opening of cave systems that trapped hominin and other fossils. Within the region, cave formation was influenced by lithological, layer-parallel controls interacting with cross-cutting fracture systems of Paleoproterozoic origin, and a NW-SE directed extensional far-field stress at a time when the African erosion surface was still intact, and elevations were probably lower. Cave geometries vary in a systematic manner across the landscape, with deep caves on the plateau and cave erosion remnants in valleys. Most caves formed to similar depths of 1400-1420 mamsl across much of the CoH, indicating that caves no longer deepened once Pliocene uplift and incision occurred, but acted as passive

  11. Reproductive Seasonality in Nesticus (Araneae: Nesticidae) Cave Spiders.

    Science.gov (United States)

    Carver, Linnea M; Perlaky, Patricia; Cressler, Alan; Zigler, Kirk S

    2016-01-01

    Spiders of the family Nesticidae are members of cave communities around the world with cave-obligate (troglobiotic) species known from North America, Europe, Asia and the Indo-Pacific. A radiation of Nesticus (Araneae: Nesticidae) in the southern Appalachians includes ten troglobiotic species. Many of these species are of conservation interest due to their small ranges, with four species being single-cave endemics. Despite conservation concerns and their important role as predators in cave communities, we know little about reproduction and feeding in this group. We addressed this knowledge gap by examining populations of two species on a monthly basis for one year. We made further observations on several other species and populations, totaling 671 individual spider observations. This more than doubled the reported observations of reproduction and feeding in troglobiotic Nesticus. Female Nesticus carry egg sacs, facilitating the determination of the timing and frequency of reproduction. We found that Nesticus exhibit reproductive seasonality. Females carried egg sacs from May through October, with a peak in frequency in June. These spiders were rarely observed with prey; only 3.3% (22/671) of individuals were observed with prey items. The frequency at which prey items were observed did not vary by season. Common prey items were flies, beetles and millipedes. Troglobiotic species constituted approximately half of all prey items observed. This result represents a greater proportion of troglobiotic prey than has been reported for various troglophilic spiders. Although our findings shed light on the life history of troglobiotic Nesticus and on their role in cave ecosystems, further work is necessary to support effective conservation planning for many of these rare species.

  12. Morphometry and distribution of isolated caves as a guide for phreatic and confined paleohydrological conditions

    Science.gov (United States)

    Frumkin, Amos; Fischhendler, Itay

    2005-04-01

    Isolated caves are a special cave type common in most karst terrains, formed by prolonged slow water flow where aggressivity is locally boosted. The morphometry and distribution of isolated caves are used here to reconstruct the paleohydrology of a karstic mountain range. Within a homogenous karstic rock sequence, two main types of isolated caves are distinguished, and each is associated with a special hydrogeologic setting: maze caves form by rising water in the confined zone of the aquifer, under the Mt. Scopus Group (Israel) confinement, while chamber caves are formed in phreatic conditions, apparently by lateral flow mixing with a vadose input from above.

  13. Radon in Austrian tourist mines and show caves

    International Nuclear Information System (INIS)

    Ringer, W.; Graeser, J.

    2009-01-01

    The radon situation in tourist mines and show caves is barely investigated in Austria. This paper investigates the influence of its determining factors, such as climate, structure and geology. For this purpose, long-term time-resolved measurements over 6 to 12 months in 4 tourist mines and 2 show caves - with 5 to 9 measuring points each - have been carried out to obtain the course of radon concentration throughout the year. In addition, temperature and air-pressure were measured and compared to the data outside where available. Results suggest that the dominating factors of the average radon concentration are structure and location (geology) of the tunnel-system, whereas the diurnal and annual variation is mainly caused by the changing airflow, which is driven by the difference in temperature inside and outside. Downcast air is connected with very low radon concentrations, upcast air with high concentrations. In some locations the maximum values appear when the airflow ceases. But airflow can be different in different parts of mines and caves. Systems close to the surface show generally lower radon levels than the ones located deeper underground. Due to variation of structure, geology and local climate, the radon situation in mines and caves can only be described by simultaneous measurements at several measuring points. (orig.)

  14. Spatial and temporal changes in invertebrate assemblage structure from the entrance to deep-cave zone of a temperate marble cave

    Directory of Open Access Journals (Sweden)

    Benjamin W. Tobin

    2013-09-01

    Full Text Available Seasonality in surface weather results in seasonal temperature and humidity changes in caves. Ecological and physiological differences among trogloxenes, troglophiles, and troglobionts result in species-dependent responses to this variability. To investigate these responses, we conducted five biological inventories in a marble cave in the Sierra Nevada Range, California, USA between May and December 2010. The cave was divided into six quadrats and temperature was continuously logged in each (humidity was logged at the entrance and in the deep cave. With increasing distance from the entrance, temperature changes were increasingly attenuated and lagged relative to surface temperature. Linear regressions were created to determine the relationship between measured environmental variables and diversity for cavernicoles (troglobionts and troglophiles and trogloxenes cave– wide and in the transition zone. Diversity for cavernicoles and trogloxenes peaked in the entrance and deep cave zones, respectively. Quadrat, date, 2-week antecedent temperature average, 2-week antecedent temperature range, and trogloxene abundance explained 76% of cavernicole diversity variability. Quadrat explained 55% of trogloxene diversity variability. In the transition zone, trogloxene abundance explained 26% of cavernicole variability and 2-week antecedent temperature and 2-week antecedent temperature range explained 40% of trogloxene variability. In the transition zone, trogloxene diversity was inversely related to 2-week antecedent temperature average and 2-week antecedent temperature range, suggesting that species were moving into the transition zone when temperature was most stable. In a CCA of cavernicoles distribution data and environmental variables, 35% of variation in species-specific distributions was attributable to quadrat, and non-significant percentages were explained by date and environmental variables. Differences in assemblage structure among quadrats were

  15. Mesolithic burial place in La Martina Cave (Dinant, Belgium)

    International Nuclear Information System (INIS)

    Dewez, M.; Gilot, E.; Groessens-Van-Dyck, M.C.; Cordy, J.M.

    1995-01-01

    The ''La Martina'' cave is located near Dinant (Belgium). Although the sediments had been shoveled out in the mid XIXth century, a calcic breccia has provided prehistoric bones. We can distinguish a Pleistocene fauna with cave bear, one Mesolithic burial place with two cromagnoid skeletons, from the 6th millennium BC, and some Holocene faunal remains. (authors). 7 refs

  16. Detection of abandoned mines/caves using airborne LWIR hyperspectral data

    Science.gov (United States)

    Shen, Sylvia S.; Roettiger, Kurt A.

    2012-09-01

    The detection of underground structures, both natural and man-made, continues to be an important requirement in both the military/intelligence and civil communities. There are estimates that as many as 70,000 abandoned mines/caves exist across the nation. These mines represent significant hazards to public health and safety, and they are of concern to Government agencies at the local, state, and federal levels. NASA is interested in the detection of caves on Mars and the Moon in anticipation of future manned space missions. And, the military/ intelligence community is interested in detecting caves, mines, and other underground structures that may be used to conceal the production of weapons of mass destruction or to harbor insurgents or other persons of interest by the terrorists. Locating these mines/caves scattered over millions of square miles is an enormous task, and limited resources necessitate the development of an efficient and effective broad area search strategy using remote sensing technologies. This paper describes an internally-funded research project of The Aerospace Corporation (Aerospace) to assess the feasibility of using airborne hyperspectral data to detect abandoned cave/mine entrances in a broad-area search application. In this research, we have demonstrated the potential utility of using thermal contrast between the cave/mine entrance and the ambient environment as a discriminatory signature. We have also demonstrated the use of a water vapor absorption line at12.55 μm and a quartz absorption feature at 9.25 μm as discriminatory signatures. Further work is required to assess the broader applicability of these signatures.

  17. Bomb-spike dating of a mummified baboon in Ludwig Cave, Namibia

    Directory of Open Access Journals (Sweden)

    Hodgins Greg

    2007-01-01

    Full Text Available In 1982 a mummified adult female baboon was discovered on a ledge in Ludwig Cave in Namibia. A toe bone was removed for dating in July 1995. AMS radiocarbon dating of bone collagen, tendon, and skin indicates a post-modern age. Application of the atomic bomb-spike calibration curve suggests death in late 1977 and an age at death of around 19 years. Baboons roost in the cave and the mummified female, along with a mummified juvenile male discovered in 2002 and three rotting corpses discovered in 1995, were probably chased by other baboons or by leopards down a ca. 6 m drop during the rainy season, and were unable to climb the steep and very slippery slope to escape. The large number of baboons trapped in the cave in less than 20 years, and mummification of two individuals on dry, dusty ledges in the cave, may explain why large numbers of baboon skeletons have been discovered in ancient bone breccias (up to 4 Ma old in a number of caves throughout Southern Africa.

  18. Radon exposure, chromosomal aberrations, and genetic polymorphisms in selected Slovak cave workers

    International Nuclear Information System (INIS)

    Musak, L.; Pec, M.; Vicanova, M.; Vodicka, P.; Hanova, M.; Buchancova, J.; Moravcikova, K.; Klimentova, G.; Vodickova, L.

    2005-01-01

    The aim of work was genotoxic risk assessment of the Slovak show cave workers employed by the Slovak Caves Administration in Liptovsky Mikulas. They are guides or administrators of the four Slovak show caves: Vazecka, Demanovska, Bystrianska, and Harmanecka. We examined 51 workers exposed to radon, with average age 35.64 years ± 6.63 (SD) and average exposition time 9.78 years ± 6.27 (SD). They are 43 men (i.e. 84.31 %) and 9 women (i.e. 15.69 %). The control group consisted of 32 healthy workers from Faculty Hospital in Martin. The workers were not exposed to any genotoxic agents. The average age is 31.84 years ± 5.84(SD). From every subject we evaluated 100 mitosis, i.e. 5100 mitosis from exposed workers and 3200 mitosis from control subjects. In exposed group we found in 111 cells chromosomal aberrations, this present 2.18 % Ab.c. ± 0.19 (SEM), and in control 1 st group 1.53 % ± 0.16 (SEM). There are 106 breaks (95.50%), and 5 exchanges (4.50%) on chromosomes. The highest number of Ab.c. we detected in workers of Vazecka (2.63 % Ab.c) and Bystrianska (2.00 % Ab.c.) caves. There is a significant increase (P < 0.05) in the mean number of Ab.c. in workers of cave Vazecka as compared to control. In 15 cases (i.e. 28.30 %) we found increase or high exposure to genotoxic agents, we found any difference between sex, and any dependence of the number of Ab.c. on age and time of exposure. The Vazecka cave workers showed three times higher mean effective doses all the year round (milliSievert) than workers additional caves. The measured values of radiation in the caves and mines exceeded the permissible limits and Regional Hygienist of the Central Slovakia declared in 1981 the risk zones and, at the same time, the monitoring of working atmosphere was initiated. Our evaluations referred to certain exposition of this carcinogen in cave workers too. The essence of prevention is based in the lowering of ionizing radiation and improvement of the sanitary

  19. The significance of the second cave episode in Jerome’s Vita Malchi

    Directory of Open Access Journals (Sweden)

    Jacobus P. Kritzinger

    2014-02-01

    Full Text Available The authors argue that the second cave episode in Jerome’s Vita Malchi Monachi Captivi should, in view of the similarities with the first cave episode and the high incidence of literary devices employed in it, be recognised for its value in the interpretation of this vita. The book was intended as a defence of, and an exhortation to a life of celibacy and this dual purpose is clearly demonstrated in both episodes in which a cave is used as the setting. The second cave episode has been neglected in the scholarly debate about the purpose of the book and this article attempts to set the record straight.

  20. Acute and sub-chronic toxicity evaluations of aqueous extract from stem bark of Grewia mollis (Malvaceae in rats

    Directory of Open Access Journals (Sweden)

    Pongri Adarki

    2017-09-01

    Full Text Available Introduction: Different parts of Grewia mollis Juss. (Malvaceae are commonly used in folk medicine to treat several ailments, including diarrhea, ulcers, rickets, cough and fever. Although several studies have proved its therapeutic effectiveness, there are very few toxicological studies on the plant. Objectives: This study was carried out to evaluate the acute and sub-chronic toxicity of the aqueous extract of G. mollis stem bark (GM in animals. Methods: In the acute study, rats were orally administrated with GM at doses of 150, 300, 600, 1200, 2400, 4800 and 9600 mg/kg to determine the oral medial lethal dose (LD50. In the chronic study, rats received three doses of GM (150, 300 and 600 mg/kg for 28 days. After the treatments, food intake, body weights, biochemical, hematological and histopathological parameters were analyzed. Results: The LD50 was estimated to be >9600 mg/kg. No significant alterations in the animal’s body weight gain, relative organs weight, serum biochemical analysis, hematological or histopathological analyses of liver, kidneys, lungs, heart and spleen were observed. Conclusions: The results of this study provided evidence that oral administration of GM at dose of 600 mg/kg is relatively safe in rats and may not exert severe toxic effects.

  1. Engineering geologic conditions at the sinkhole entrance to Logan Cave, Benton County, Arkansas

    Science.gov (United States)

    Schulz, William H.; McKenna, Jonathan P.

    2004-01-01

    Logan Cave, located in Benton County, Arkansas, is inhabited by several endangered and threatened species. The cave and surrounding area was designated a National Wildlife Refuge under the control of the U.S. Fish and Wildlife Service (USFWS) in 1989. Cave researchers access the cave through a steep-sided sinkhole entrance, which also is one of the two access points used by endangered bats. There is evidence of instability of one of the entrance slopes that has raised concerns that the entrance could close if slope failure was to occur. At the request of USFWS, we performed an engineering geologic investigation of the sinkhole to evaluate stability of this slope, which is comprised of soil, and other mechanisms of sediment transport into the cave entrance. The investigation included engineering geologic mapping, sampling and laboratory testing of subsurface geologic materials, and slope-stability analysis. We found that the sinkhole slope that extends into the entrance of the cave is comprised of sandy and gravelly soil to the depths explored (6.4 meters). This soil likely was deposited as alluvium within a previous, larger sinkhole. Based on properties of the alluvium, geometry of the slope, and results of finite-element slope-stability analyses, we conclude that the slope is marginally stable. Future failures of the slope probably would be relatively thin and small, thus several would be required to completely close the cave entrance. However, sediment is accumulating within the cave entrance due to foot traffic of those accessing the cave, surface-water erosion and transport, and shallow slope failures from the other sinkhole slopes. We conclude that the entrance will be closed by sediment in the future, similar to another entrance that we identified that completely closed in the past. Several measures could be taken to reduce the potential for closure of the cave entrance, including periodic sediment removal, installation of materials that reduce erosion by

  2. Seasonal variations of {sup 14}C and δ{sup 13}C for cave drip waters in Ryugashi Cave, Shizuoka Prefecture, central Japan

    Energy Technology Data Exchange (ETDEWEB)

    Minami, Masayo, E-mail: minami@nendai.nagoya-u.ac.jp [Center for Chronological Research, Nagoya University, Nagoya 464-8602 (Japan); Kato, Tomomi [Graduate School of Environmental Studies, Nagoya University, Nagoya 464-8601 (Japan); Horikawa, Keiji [Department of Environmental Biology and Chemistry, Toyama University, Toyama 930-8555 (Japan); Nakamura, Toshio [Center for Chronological Research, Nagoya University, Nagoya 464-8602 (Japan)

    2015-11-01

    Speleothem {sup 14}C has recently emerged as a potentially powerful proxy for hydrology changes in comparison with atmospheric {sup 14}C calibration curve, rather than as a direct dating tool, apart from a time marker using bomb peak of {sup 14}C. Some possible causes for the relationship between speleothem {sup 14}C content (or dead carbon fraction: DCF) and karst hydrology have been proposed, such as changes in temperature, precipitation, drip water flow dynamics, cave air ventilation, soil air pCO{sub 2}. In this study, we investigated seasonal variation in {sup 14}C and δ{sup 13}C of drip water in Ryugashi Cave, Shizuoka Prefecture, central Japan, to examine the causes of the {sup 14}C and δ{sup 13}C variations in a speleothem. The results show that different {sup 14}C concentrations and δ{sup 13}C values of drip water from the Ryugashi Cave, were exhibited at different sites of the Caves No. 1, No. 3, and No. 4, which have different temperature, air pCO{sub 2}, and flow paths. Further, the {sup 14}C and δ{sup 13}C of drip waters showed seasonal variations at all sites, which were lower in fall and winter, and higher in spring and summer, though the extent of the variations was different among the sites. The {sup 14}C in drip waters tended to be correlated with the drip rates: {sup 14}C tended to be higher in drip waters with higher drip rates, and also correlated with rainfall amount around the Ryugashi Cave, especially for the drip waters in Cave No. 3, which are considered to have simpler flow paths. The increase in rainfall amount could bring the increase in drip rate of drip water, and then the decrease in interaction between solution and karst, resulting in {sup 14}C increase (DCF decrease) in drip water. Accordingly, the reconstruction of precipitation could be performed using {sup 14}C variation in a speleothem formed by drip water with simple flow dynamics.

  3. The microbiology of Lascaux Cave

    Czech Academy of Sciences Publication Activity Database

    Bastian, F.; Jurado, V.; Nováková, Alena; Alabouvette, C.; Saiz-Jimenez, C.

    2010-01-01

    Roč. 156, č. 3 (2010), s. 644-652 ISSN 1350-0872 Institutional research plan: CEZ:AV0Z60660521 Keywords : Lascaux Cave * microbiology * Paleolithic paintings Subject RIV: EH - Ecology, Behaviour Impact factor: 2.957, year: 2010

  4. In situ acetylene reduction activity of Scytonema julianum in Vapor cave (Spain

    Directory of Open Access Journals (Sweden)

    Asencio Antonia Dolores

    2011-01-01

    Full Text Available Nitrogen fixation was measured in situ for the first time by acetylene reduction for a greyish mat composed of Scytonema julianum in cave- like environments. Mat-specific rates (129.9-215.7 nmol C2 H4 m-2 s-1 for daytime fixation and 65.1-120.6 nmol C2 H4 m-2 s-1 for nighttime fixation recorded in the Vapor cave differed considerably due to the energy reserves stored during photosynthesis being exhausted and used in the dark phase. The most influential environmental parameter for nitrogen fixation in the Vapor cave is temperature in the daytime and nighttime fixations. Nitrogen fixation by cyanobacteria may contribute considerably to the overall nitrogen cycle in harsh environments such as caves. Nitrogenase activity in Scytonema julianum was roughly 30 times higher than that of Scytonema mirabile, which also grew in cave environments, which is due to the characteristics of each site. The entrance of Vapour cave (Spain faces SE, measures 0.75 x 0.6 m and opens to shafts of a total depth of 80 m. Its dimensions and environmental conditions (relative humidity up to 100%; maximum temperature, 43oC imply that it is isolated from external influences, and that the microclimate differs substantially from that experienced externally. Nitrogen fixation, photon flux density, relative humidity and temperature in the Vapor cave were taken hourly over a 24-hour period in winter.

  5. Organochlorine residues in bat guano from nine Mexican caves, 1991

    Science.gov (United States)

    Clark, D.R.; Moreno-Valdez, A.; Mora, M.A.

    1995-01-01

    Samples of bat guano, primarily from Mexican free-tailed bats (Tadarida brasiliensis), were collected at nine bat roosts in caves in northern and eastern Mexico and analysed for organochlorine residues. DDE, the most abundant residue found in each cave, was highest (0.99 p.p.m. dry weight) at Ojuela Cave, Durango. Other studies of DDE in bat guano indicate that this concentration is too low to reflect harmful concentrations in the bats themselves. The DDE at Ojuela may represent either lingering residues from use of DDT years ago in the Ojuela area of perhaps depuration loss from migrant bats with summer maternity roost(s) in a DDE-contaminated area such as Carlsbad Cavern, New Mexico. Presence of o,p-DDT at Tio Bartolo Cave, Nuevo Leon, indicates recent use of DDT, but the concentration of this contaminant was low. Possible impacts on bat colonies of the organophosphorus and carbamate insecticides now in extensive use are unknown.

  6. Predator avoidance in extremophile fish.

    Science.gov (United States)

    Bierbach, David; Schulte, Matthias; Herrmann, Nina; Zimmer, Claudia; Arias-Rodriguez, Lenin; Indy, Jeane Rimber; Riesch, Rüdiger; Plath, Martin

    2013-02-06

    Extreme habitats are often characterized by reduced predation pressures, thus representing refuges for the inhabiting species. The present study was designed to investigate predator avoidance of extremophile populations of Poecilia mexicana and P. sulphuraria that either live in hydrogen sulfide-rich (sulfidic) springs or cave habitats, both of which are known to have impoverished piscine predator regimes. Focal fishes that inhabited sulfidic springs showed slightly weaker avoidance reactions when presented with several naturally occurring predatory cichlids, but strongest differences to populations from non-sulfidic habitats were found in a decreased shoaling tendency with non-predatory swordtail (Xiphophorus hellerii) females. When comparing avoidance reactions between P. mexicana from a sulfidic cave (Cueva del Azufre) and the adjacent sulfidic surface creek (El Azufre), we found only slight differences in predator avoidance, but surface fish reacted much more strongly to the non-predatory cichlid Vieja bifasciata. Our third experiment was designed to disentangle learned from innate effects of predator recognition. We compared laboratory-reared (i.e., predator-naïve) and wild-caught (i.e., predator-experienced) individuals of P. mexicana from a non-sulfidic river and found no differences in their reaction towards the presented predators. Overall, our results indicate (1) that predator avoidance is still functional in extremophile Poecilia spp. and (2) that predator recognition and avoidance reactions have a strong genetic basis.

  7. Predator Avoidance in Extremophile Fish

    Science.gov (United States)

    Bierbach, David; Schulte, Matthias; Herrmann, Nina; Zimmer, Claudia; Arias-Rodriguez, Lenin; Indy, Jeane Rimber; Riesch, Rüdiger; Plath, Martin

    2013-01-01

    Extreme habitats are often characterized by reduced predation pressures, thus representing refuges for the inhabiting species. The present study was designed to investigate predator avoidance of extremophile populations of Poecilia mexicana and P. sulphuraria that either live in hydrogen sulfide-rich (sulfidic) springs or cave habitats, both of which are known to have impoverished piscine predator regimes. Focal fishes that inhabited sulfidic springs showed slightly weaker avoidance reactions when presented with several naturally occurring predatory cichlids, but strongest differences to populations from non-sulfidic habitats were found in a decreased shoaling tendency with non-predatory swordtail (Xiphophorus hellerii) females. When comparing avoidance reactions between P. mexicana from a sulfidic cave (Cueva del Azufre) and the adjacent sulfidic surface creek (El Azufre), we found only slight differences in predator avoidance, but surface fish reacted much more strongly to the non-predatory cichlid Vieja bifasciata. Our third experiment was designed to disentangle learned from innate effects of predator recognition. We compared laboratory-reared (i.e., predator-naïve) and wild-caught (i.e., predator-experienced) individuals of P. mexicana from a non-sulfidic river and found no differences in their reaction towards the presented predators. Overall, our results indicate (1) that predator avoidance is still functional in extremophile Poecilia spp. and (2) that predator recognition and avoidance reactions have a strong genetic basis. PMID:25371337

  8. Microbial communities in dark oligotrophic volcanic ice cave ecosystems of Mt. Erebus, Antarctica

    Directory of Open Access Journals (Sweden)

    Bradley M. Tebo

    2015-03-01

    Full Text Available The Earth’s crust hosts a subsurface, dark, and oligotrophic biosphere that is poorly understood in terms of the energy supporting its biomass production and impact on food webs at the Earth’s surface. Dark oligotrophic volcanic ecosystems (DOVEs are good environments for investigations of life in the absence of sunlight as they are poor in organics, rich in chemical reactants and well known for chemical exchange with Earth’s surface systems. Ice caves near the summit of Mt. Erebus (Antarctica offer DOVEs in a polar alpine environment that is starved in organics and with oxygenated hydrothermal circulation in highly reducing host rock. We surveyed the microbial communities using PCR, cloning, sequencing and analysis of the small subunit (16S ribosomal and Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (RubisCO genes in sediment samples from three different caves, two that are completely dark and one that receives snow-filtered sunlight seasonally. The microbial communities in all three caves are composed primarily of Bacteria and fungi; Archaea were not detected. The bacterial communities from these ice caves display low phylogenetic diversity, but with a remarkable diversity of RubisCO genes including new deeply branching Form I clades, implicating the Calvin-Benson-Bassham cycle as a pathway of CO2 fixation. The microbial communities in one of the dark caves, Warren Cave, which has a remarkably low phylogenetic diversity, were analyzed in more detail to gain a possible perspective on the energetic basis of the microbial ecosystem in the cave. Atmospheric carbon (CO2 and CO, including from volcanic emissions, likely supplies carbon and/or some of the energy requirements of chemoautotrophic microbial communities in Warren Cave and probably other Mt. Erebus ice caves. Our work casts a first glimpse at Mt. Erebus ice caves as natural laboratories for exploring carbon, energy and nutrient sources in the subsurface biosphere and the

  9. Effects of the 2,4-D herbicide on gills epithelia and liver of the fish Poecilia vivipara

    Directory of Open Access Journals (Sweden)

    Ana F. Vigário

    2014-06-01

    Full Text Available The 2,4-dichlorophenoxyacetic acid, usually named 2,4-D is one of the most widely used herbicides in the world. Acute toxicity of 2,4-D herbicide was investigated through its effects on guppies (Poecilia vivipara Bloch et Schneider 1801. Fish were exposed to the herbicide at concentrations of 10, 20 and 40µl per liter of water for 24 hours to determine its effects on gills and liver epithelia. The estimated LC50 was 34.64µl of 2,4-D per liter of water. Histochemical analyses and Feulgen's reaction were conducted to detect glycoconjugates and DNA, respectively, in gills and liver epithelia. Histochemistry revealed qualitative variations of glycoconjugates present on mucous cells and granules. The four types of mucous cells contained neutral granules, acids, or both. Increasing amounts of syalomucins were observed from the control group to the group exposed to the highest concentration of 2,4-D, suggesting increased mucous viscosity and the formation of plaques that could inhibit gas exchange and osmoregulation. Lamellar fusion observed in the group exposed to 40µl of 2,4-D suggests a defense mechanism. Hepatocytes showed vacuolization in the 10 and 20µl/L groups. The 40 µl/L group showed normal hepatocytes as well as changed ones, many Ito cells, micronuclei, and nuclear swelling. These effects may be associated with toxicity or adaptative processes to cellular stress. The data from this study indicates the importance of assessing similar risks to aquatic species and suggests that Poecilia vivipara is an adequate biological model for analysis of environmental contamination.

  10. Genome-scale data reveal that endemic Poecilia populations from small sulphidic springs display no evidence of inbreeding.

    Science.gov (United States)

    Brown, Anthony P; Greenway, Ryan; Morgan, Samuel; Quackenbush, Corey R; Giordani, Luca; Arias-Rodriguez, Lenin; Tobler, Michael; Kelley, Joanna L

    2017-10-01

    Populations with limited ranges can be highly vulnerable to changes in their environment and are, thus, of high conservation concern. Populations that experience human-induced range reductions are often highly inbred and lack genetic diversity, but it is unknown whether this is also the case for populations with naturally small ranges. The fishes Poecilia sulphuraria (listed as critically endangered) and Poecilia thermalis, which are endemic to small hydrogen sulphide-rich springs in southern Mexico, are examples of such populations with inherently small habitats. We used geometric morphometrics and population genetics to quantify phenotypic and genetic variation within and among two populations of P. sulphuraria and one population of P. thermalis. Principal component analyses revealed phenotypic and genetic differences among the populations. Evidence for inbreeding was low compared to populations that have undergone habitat reduction. The genetic data were also used to infer the demographic history of these populations to obtain estimates for effective population sizes and migration rates. Effective population sizes were large given the small habitats of these populations. Our results imply that these three endemic extremophile populations should each be considered separately for conservation purposes. Additionally, this study suggests that populations in naturally small habitats may have lower rates of inbreeding and higher genetic diversity than expected, and therefore may be better equipped to handle environmental perturbations than anticipated. We caution, however, that the inferred lack of inbreeding and the large effective population sizes could potentially be a result of colonization by genetically diverse ancestors. © 2017 John Wiley & Sons Ltd.

  11. Recreational Impacts on the Microclimate of the Gorilla Limestone Cave in Shoushan National Nature Park of Taiwan

    Science.gov (United States)

    Chen, Chun; Ho, Lih-Der

    2017-04-01

    This study reports a continuous microclimate monitoring carried out in the Gorilla Cave (Kaohsiung, Taiwan) between December 2015 and December 2016. This limestone cave is located in the Mt. Shoushan, which is mainly composed of limestone and mudstone. This study tried to assess the recreational impacts to the microclimate of the cave by monitoring the CO2, temperature, humidity and barometric pressure. Two monitoring stations were set up respectively at the front part (station A) and the end of the cave (station B). We also set up an auto-operated time-lapse camera at the entrance of the cave to record the numbers of tourists, and their entering time and the durations in cave. As carbon dioxide in the limestone cave may have negative impact to both speleothems and visitors, our presentation focuses on the variations of CO2 concentration in the Gorilla Cave. Daily and seasonal fluctuations of CO2 concentration were observed. The fluctuations are closely related with the temperature outside the cave. In summer, when the temperature outside the cave maintained at 30。C, fluctuations of CO2 concentration in the cave will become chaotic. The CO2 concentration would fluctuate around 1000ppm most of the day, but it would be relatively low ( 500ppm) during the noon. In winter, when temperature outside the cave maintained below 25゜C, the fluctuation of CO2 concentration in cave presented a steady state ( 400-500 ppm). Only at the noon, the temperature outside the cave rose above 25 ゜C, the CO2 concentration inside the cave would increase. There were 1,517 tourists entered the cave during the monitoring period. The average number of visitors in a group is 13, and each group averagely stayed for 15 minutes. Over half of the visitors (776 tourists) entered the cave in December, due to lower humidity, drier in the cave and less dripping water in winter. After tourists entered the cave, the CO2 concentration value of station A rose instantly. However, most tourists stayed

  12. Experimental Research on Internal Behaviors of Caved Rocks under the Uniaxial Confined Compression

    Directory of Open Access Journals (Sweden)

    Yu-jiang Zhang

    2017-01-01

    Full Text Available As main composition of longwall gob, caved rocks’ behaviors and their impacts under compression crucially influence strata control, subsidence, associated resources extraction, and many other aspects. However, current researches are based on a whole sample, due to looseness of caved rocks and limitation of observation technology. In this paper, an experiment system was built to investigate internal behaviors of caved rocks’ sample, under the uniaxial confined compression, including movement and breakage behavior by the digital image processing technologies. The results show that the compression process of caved rocks could be divided into two stages by relative density. Boundary effect and changes of voids and contact pressure among caved rocks lead to different movement law in different position in sample’s interior. A stratification phenomenon of breakage was discovered, which presents breakage concentration in the middle of the sample. The nonlinear movement and shear dislocation induced by shifts among caved rocks are the reason of the breakage stratification phenomenon. This phenomenon would have an effect on the permeability and seepage research of similar medium.

  13. Reconciling Mining with the Conservation of Cave Biodiversity: A Quantitative Baseline to Help Establish Conservation Priorities.

    Science.gov (United States)

    Jaffé, Rodolfo; Prous, Xavier; Zampaulo, Robson; Giannini, Tereza C; Imperatriz-Fonseca, Vera L; Maurity, Clóvis; Oliveira, Guilherme; Brandi, Iuri V; Siqueira, José O

    2016-01-01

    Caves pose significant challenges for mining projects, since they harbor many endemic and threatened species, and must therefore be protected. Recent discussions between academia, environmental protection agencies, and industry partners, have highlighted problems with the current Brazilian legislation for the protection of caves. While the licensing process is long, complex and cumbersome, the criteria used to assign caves into conservation relevance categories are often subjective, with relevance being mainly determined by the presence of obligate cave dwellers (troglobites) and their presumed rarity. However, the rarity of these troglobitic species is questionable, as most remain unidentified to the species level and their habitats and distribution ranges are poorly known. Using data from 844 iron caves retrieved from different speleology reports for the Carajás region (South-Eastern Amazon, Brazil), one of the world's largest deposits of high-grade iron ore, we assess the influence of different cave characteristics on four biodiversity proxies (species richness, presence of troglobites, presence of rare troglobites, and presence of resident bat populations). We then examine how the current relevance classification scheme ranks caves with different biodiversity indicators. Large caves were found to be important reservoirs of biodiversity, so they should be prioritized in conservation programs. Our results also reveal spatial autocorrelation in all the biodiversity proxies assessed, indicating that iron caves should be treated as components of a cave network immersed in the karst landscape. Finally, we show that by prioritizing the conservation of rare troglobites, the current relevance classification scheme is undermining overall cave biodiversity and leaving ecologically important caves unprotected. We argue that conservation efforts should target subterranean habitats as a whole and propose an alternative relevance ranking scheme, which could help simplify the

  14. Geochemical characteristics of cave drip water respond to ENSO based on a 6-year monitoring work in Yangkou Cave, Southwest China

    Science.gov (United States)

    Chen, Chao-Jun; Li, Ting-Yong

    2018-06-01

    The scientific explanation of speleothem δ18O in Chinese monsoon region is a greatly debated issue. Modern cave monitoring combined with instrument observation maybe is an essential solution to deal with this issue. During the period from 2011 to 2016, we monitored local precipitation, soil water in three soil profiles, and six drip water sites in Yangkou Cave, which is located in Chongqing City, Southwest China. This article presents measurements about δ18O, δD and Mg/Ca ratios of drip water and compared these geochemical proxies with contemporaneous atmospheric circulations. The main conclusions are: (1) As water migrates from precipitation to soil water to cave drip water, the amplitudes of seasonal variations in δD and δ18O decreased gradually. Due to the existence of complex hydrogeological conditions, the range of variation and the seasonal characteristics of δD and δ18O differ among the drip sites where samples were collected, but the interannual variability is nearly the same. The drip water Mg/Ca ratios are mainly regulated by changes in hydrological conditions in the epikarst zone, with higher values during winter months than that during summer months. (2) When an El Niño event occurs, the Western Pacific Subtropical High (WPSH) is migrated westward, and the production of near-source water vapor from the western Pacific and the South China Sea increases, leading to higher δ18O values in the precipitation and the cave drip water. The drip water Mg/Ca ratios were significantly lower with increased summer precipitation. On the other hand, during La Niña events, the WPSH is migrated eastward, and inputs of water vapor that has traveled greater distances (from the Indian Ocean) become comparatively important, resulting in lower δ18O values in the precipitation and the cave drip water. The drip water Mg/Ca ratios were higher with decreased summer precipitation. In summary, the interannual variability of δ18O in the drip waters of Yangkou Cave

  15. Flash Extraction and Physicochemical Characterization of Oil from Elaeagnus mollis Diels Seeds.

    Science.gov (United States)

    Kan, Lina; Wang, Lin; Ding, Qingzhen; Wu, Yanwen; Ouyang, Jie

    2017-04-03

    A flash extraction method was used to isolate Elaeagnus mollis oil (EMO). The optimal extraction parameters, sample/solvent ratio and extraction temperature, were determined to be 1:10 (g/mL) and 40°C, respectively. Especially, the extraction yield reached 49.30% when the extraction time was as short as 2 min. No obvious difference was observed in fatty acid composition, iodine value, saponification number, total phenolic content and tocopherol content between flash-extracted EMO and Soxhlet-extracted EMO, but their physicochemical values were lower than those of cold-pressed EMO. Cold-pressed EMO had higher oxidation stability, DPPH (1-diphenyl-2-picrylhydrazyl) and hydroxyl radical-scavenging activities than flash-extracted EMO and Soxlet extracted EMO. The flash extraction is demonstrated to be an alternative, efficient method for the vegetable oil production.

  16. Composición Química y Actividad Antibacteriana del Aceite Esencial de Minthostachys mollis

    OpenAIRE

    Torrenegra-Alarcón, Miladys; Granados-Conde, Clemente; Durán-Lengua, Marlene; León-Méndez, Glicerio; Yáñez-Rueda, Xiomara; Martínez, César; Pájaro-Castro, Nerlis

    2016-01-01

    Se evaluó la composición química y la actividad antibacteriana del aceite esencial Minthostachys mollis cultivado en el departamento de Norte de Santander, Colombia.El aceite esencial fue obtenido por hidrodestilación convencional, a partir de las hojas; se determinó densidad relativa a 20°C, índice de refracción; solubilidad de los aceites esenciales en etanol (70% v/v) y rotación óptica. La composición química se evaluó mediante cromatografía de gases/espectrómetro de masa. La actividad se ...

  17. Orthothermographies and 3D modeling as potential tools in ice caves studies: the Peña Castil Ice Cave (Picos de Europa, Northern Spain

    Directory of Open Access Journals (Sweden)

    Fernando Berenguer-Sempere

    2014-01-01

    Full Text Available Currently there are many studies focused on the investigation of climatic and glaciological condition of ice caves. Here we present another way to address these studies, applying some methods already used in fields other than geomorphology. The versatility and accuracy provided by the use of modern topography and thermography techniques, using Terrestrial Laser Scanner and current thermographic cameras- and the creation of 3D thermographic models and orthothermographies derived from them - is shown to be a useful tool as it is difficult to obtain data from fieldwork and traditional methods used in caves. This paper presents the potential uses of combined TLS and thermographic techniques for monitoring some important climatological parameters in the sensitive periglacial environment of the Iberian Atlantic high mountains: Peña Castil Ice Cave (Picos de Europa, Northern Spain. A systematic application of such combined technologies to these kind of caves, is expected to contribute to a quantitative and concise characterization of the evolution of the ice as shown by the results of this study.

  18. Diversity and role of cave-dwelling hematophagous insects in pathogen transmission in the Afrotropical region.

    Science.gov (United States)

    Obame-Nkoghe, Judicaël; Leroy, Eric-Maurice; Paupy, Christophe

    2017-04-12

    The progressive anthropization of caves for food resources or economic purposes increases human exposure to pathogens that naturally infect cave-dwelling animals. The presence of wild or domestic animals in the immediate surroundings of caves also may contribute to increasing the risk of emergence of such pathogens. Some zoonotic pathogens are transmitted through direct contact, but many others require arthropod vectors, such as blood-feeding insects. In Africa, hematophagous insects often play a key role in the epidemiology of many pathogens; however, their ecology in cave habitats remains poorly known. During the last decades, several investigations carried out in Afrotropical caves suggested the medical and veterinary importance particularly of insect taxa of the Diptera order. Therefore, the role of some of these insects as vectors of pathogens that infect cave-dwelling vertebrates has been studied. The present review summarizes these findings, brings insights into the diversity of cave-dwelling hematophagous Diptera and their involvement in pathogen transmission, and finally discusses new challenges and future research directions.

  19. Mapping planetary caves with an autonomous, heterogeneous robot team

    Science.gov (United States)

    Husain, Ammar; Jones, Heather; Kannan, Balajee; Wong, Uland; Pimentel, Tiago; Tang, Sarah; Daftry, Shreyansh; Huber, Steven; Whittaker, William L.

    Caves on other planetary bodies offer sheltered habitat for future human explorers and numerous clues to a planet's past for scientists. While recent orbital imagery provides exciting new details about cave entrances on the Moon and Mars, the interiors of these caves are still unknown and not observable from orbit. Multi-robot teams offer unique solutions for exploration and modeling subsurface voids during precursor missions. Robot teams that are diverse in terms of size, mobility, sensing, and capability can provide great advantages, but this diversity, coupled with inherently distinct low-level behavior architectures, makes coordination a challenge. This paper presents a framework that consists of an autonomous frontier and capability-based task generator, a distributed market-based strategy for coordinating and allocating tasks to the different team members, and a communication paradigm for seamless interaction between the different robots in the system. Robots have different sensors, (in the representative robot team used for testing: 2D mapping sensors, 3D modeling sensors, or no exteroceptive sensors), and varying levels of mobility. Tasks are generated to explore, model, and take science samples. Based on an individual robot's capability and associated cost for executing a generated task, a robot is autonomously selected for task execution. The robots create coarse online maps and store collected data for high resolution offline modeling. The coordination approach has been field tested at a mock cave site with highly-unstructured natural terrain, as well as an outdoor patio area. Initial results are promising for applicability of the proposed multi-robot framework to exploration and modeling of planetary caves.

  20. Biochemical mechanisms of resistance to p-nitrochlorobenzene of karst caves microorganisms

    Directory of Open Access Journals (Sweden)

    O. S. Suslova

    2015-08-01

    Full Text Available The biochemical mechanisms of resistance to persistent organic xenobiotic p-nitrochlorobenzene (NCB of bacterial strains isolated from two cave clays ecosystems – Mushkarova Yama (Podolia, Ukraine and Kuybyshevskaya (Western Caucasus, Abkhazia have been established. It has been determined that chemoorganotrophic karst caves strains could interact with NCB and transform it reducing the nitro group with formation of p-chloroaniline (ClA followed by further destruction of NCB aromatic ring. This explained high resistance of caves strains to NCB. The studied strains could potentially be used in wastewater treatment from nitrochloraromatic compounds.

  1. Occurrence of organic wastewater and other contaminants in cave streams in northeastern Oklahoma and northwestern Arkansas

    Science.gov (United States)

    Bidwell, Joseph R.; Becker, C.; Hensley, S.; Stark, R.; Meyer, M.T.

    2010-01-01

    The prevalence of organic wastewater compounds in surface waters of the United States has been reported in a number of recent studies. In karstic areas, surface contaminants might be transported to groundwater and, ultimately, cave ecosystems, where they might impact resident biota. In this study, polar organic chemical integrative samplers (POCISs) and semipermeable membrane devices (SPMDs) were deployed in six caves and two surface-water sites located within the Ozark Plateau of northeastern Oklahoma and northwestern Arkansas in order to detect potential chemical contaminants in these systems. All caves sampled were known to contain populations of the threatened Ozark cavefish (Amblyopsis rosae). The surface-water site in Oklahoma was downstream from the outfall of a municipal wastewater treatment plant and a previous study indicated a hydrologic link between this stream and one of the caves. A total of 83 chemicals were detected in the POCIS and SPMD extracts from the surface-water and cave sites. Of these, 55 chemicals were detected in the caves. Regardless of the sampler used, more compounds were detected in the Oklahoma surface-water site than in the Arkansas site or the caves. The organic wastewater chemicals with the greatest mass measured in the sampler extracts included sterols (cholesterol and ??-sitosterol), plasticizers [diethylhexylphthalate and tris (2-butoxyethyl) phosphate], the herbicide bromacil, and the fragrance indole. Sampler extracts from most of the cave sites did not contain many wastewater contaminants, although extracts from samplers in the Oklahoma surfacewater site and the cave hydrologically linked to it had similar levels of diethylhexyphthalate and common detections of carbamazapine, sulfamethoxazole, benzophenone, N-diethyl-3-methylbenzamide (DEET), and octophenol monoethoxylate. Further evaluation of this system is warranted due to potential ongoing transport of wastewaterassociated chemicals into the cave. Halogenated organics

  2. Application of Ground Penetrating Radar Supported by Mineralogical-Geochemical Methods for Mapping Unroofed Cave Sediments

    Directory of Open Access Journals (Sweden)

    Teja Čeru

    2018-04-01

    Full Text Available Ground penetrating radar (GPR using a special unshielded 50 MHz Rough Terrain Antenna (RTA in combination with a shielded 250 MHz antenna was used to study the capability of this geophysical method for detecting cave sediments. Allochthonous cave sediments found in the study area of Lanski vrh (W Slovenia are now exposed on the karst surface in the so-called “unroofed caves” due to a general lowering of the surface (denudation of carbonate rocks and can provide valuable evidence of the karst development. In the first phase, GPR profiles were measured at three test locations, where cave sediments are clearly evident on the surface and appear with flowstone. It turned out that cave sediments are clearly visible on GPR radargrams as areas of strong signal attenuation. Based on this finding, GPR profiling was used in several other places where direct indicators of unroofed caves or other indicators for speleogenesis are not present due to strong surface reshaping. The influence of various field conditions, especially water content, on GPR measurements was also analysed by comparing radargrams measured in various field conditions. Further mineralogical-geochemical analyses were conducted to better understand the factors that influence the attenuation in the area of cave sediments. Samples of cave sediments and soils on carbonate rocks (rendzina were taken for X-ray diffraction (XRD and X-ray fluorescence (XRF analyses to compare the mineral and geochemical compositions of both sediments. Results show that cave sediments contain higher amounts of clay minerals and iron/aluminium oxides/hydroxides which, in addition to the thickness of cave sediments, can play an important role in the depth of penetration. Differences in the mineral composition also lead to water retention in cave sediments even through dry periods which additionally contribute to increased attenuation with respect to surrounding soils. The GPR method has proven to be reliable for

  3. The chronology of Moncks Cave, Canterbury, New Zealand

    International Nuclear Information System (INIS)

    Jacomb, C.

    2008-01-01

    Moncks Cave is a key site in understanding the nature and course of change to Maori culture during the early period of New Zealand prehistory because of the range of both perishable and non-perishable artefacts found there in 1889. Understood to have been completely excavated at that time, the interpretation of the material culture of the site has been rendered difficult by the absence of chronological or stratigraphic provenance data. Recent investigations at Moncks Cave revealed several intact cultural deposits, including both faunal and artefactual remains. Eleven radiocarbon determinations on marine shell suggest that the cave was occupied some time between the mid-fourteenth and mid-fifteenth centuries AD. Although the dates cannot be directly correlated with any particular artefact, the results have important implications for the interpretation of the place of the site and its contents as a whole in the context of the New Zealand prehistoric sequence. (author). 22 refs., 5 figs., 1 tab

  4. The Caves of Naica: a decade of research

    International Nuclear Information System (INIS)

    Gazquez, F.; Calaforra, J. M.; Porti, P.; Badino, G.

    2016-01-01

    The caves of the Naica Mine have been the subject of study by scientists from up to seven counties over the past decade. Up to fifty research works have published to date, most relating to the origin of the giant selenite crystals of the Cueva de los Cristales. Nevertheless, a great deal of knowledge has been generated about other relevant aspects of the Naica system. This paper puts together the vast information available about the Naica caves, from the discovery of the Cueva de los Cristales in 2000 to the more recent investigations addressing mineralogy, microclimatology and the use of gypsum speleothems as a palaeo-environmental proxy. Special attention has been paid to novel research lines that have started to use the speleothems of Naica as a study case, particularly in fields such as Astrobiology and Planetary geology. Moreover, the conservation challenges which these caves will face in the near future as consequence of the end of mining activities have also been addressed in this article. (Author)

  5. Cave-dwelling bats (Mammalia: Chiroptera and conservation concerns in South central Mindanao, Philippines

    Directory of Open Access Journals (Sweden)

    Krizler C. Tanalgo

    2015-12-01

    Full Text Available The stable microclimate in caves provides a relatively constant habitat for many bat species in the Philippines, but human encroachment continues to disrupt this habitat and imperil many of the species roosting in the caves.  In South central Mindanao, the diversity and conservation status of cave bats remain undocumented and unexplored.  We employed mist-netting to capture bats from five different caves within the town of Kabacan, northern Cotabato, Philippines.  A total of 14 bat species were identified including the Philippine endemics Hipposideros pygmaeus and Ptenochirus jagori and the threatened Megaerops wetmorei. However, despite the declining conservation status of the bats, local disturbance such as bat hunting for bush meat and unregulated tourism are currently taking place in the caves.  Large species such as Eonycteris spelaea and Rousettus amplexicaudatus are killed almost every day for food and trade.  Therefore, the high species richness, and the presence of endemic and threatened species coupled with the occurrence of anthropogenic disturbances in caves suggests the need for an urgent and effective conservation intervention involving the local government and public community. 

  6. Preference, tolerance and resistance responses of Poecilia sphenops Valenciennes, 1846 (Pisces: Poeciliidae to thermal fluctuations Respuesta de preferencia, tolerancia y resistencia de Poecilia sphenops Valenciennes, 1846 (Pisces: Poeciliidae a fluctuaciones térmicas

    Directory of Open Access Journals (Sweden)

    Mónica Hernández-Rodríguez

    2010-01-01

    Full Text Available Poecilia sphenops was acclimated to two thermal fluctuations, each following a symmetrical and asymmetrical cycle. The critical maximum temperatures of the fish were significantly different (P Poecilia sphenops fue aclimatada a dos fluctuaciones térmicas cada una con un ciclo simétrico y asimétrico. La temperatura crítica maxima de los peces fue significativamente diferente (P < 0.001 entre las fluctuaciones y se observó a 40°C en el ciclo 20-29°C; para el ciclo 25-35°C fue mayor a 42°C. Los peces aclimatados a la fluctuación 20-29°C fueron más resistentes al frío con una temperatura crítica mínima de 10°C, en contraste a los 11.7°C del régimen 26-35°C. En ambos ciclos la temperatura letal incipiente superior tuvo un intervalo de 38.8 a 39.5°C y la temperatura letal incipiente inferior fue de 10.8 a 11.8°C. La temperatura preferida de las hembras fue de 30 a 31°C, con excepción del ciclo simétrico 26-35°C. La temperatura preferida de los machos aclimatados al ciclo simétrico fue de 23.8 a 24.2°C y de 27.4 a 29.4°C en el ciclo asimétrico. Las temperaturas de evitación de los machos y las hembras aclimatados a la fluctuación 20-29°C tienen un intervalo de 14 a 16°C comparado con el intervalo de 8.4 all .4°C del tratamiento 26-35°C. Los resultados indican que P. sphenops está perfectamente adaptado a las marcadas estaciones de lluvia y sequía de su habitat.

  7. Geological constraints on cave development in the plateau-gorge karst of South China (Wulong, Chongqing)

    Science.gov (United States)

    Szczygieł, Jacek; Golicz, Mateusz; Hercman, Helena; Lynch, Erin

    2018-03-01

    The Houping Tiankeng cluster is a part of the South China Karst UNESCO World Natural Heritage Site. Within the distinctive Wulong plateau-gorge karst, > 200 km of cave passages have been documented to date. This paper focuses on detailed tectonic and morphological research on the Luo Shui Kong cave, enriched with U-series dating of speleothems and complemented by morphometric analysis of the San Wang Dong and Er Wang Dong caves. All of these caves exhibit three regional levels of cave development: 1) 1040-1020 m a.s.l.; 2) 900-840 m a.s.l.; and 3) 740-660 m a.s.l. The Houping Tiankeng area is a carbonate rock sequence several hundred meters thick, overlain by the less soluble Lower Ordovician strata, limiting recharge points to faults exposing underlying easily soluble formations. This leads to the domination of concentrated, high-volume inflow and thus results in caves of large volume in the plateau-gorge karst. Shafts connecting the surface with cave passages located underneath formed along faults, changing the hydrogeological pattern through karst water capture and remodeling of existing conduits, albeit mainly by increasing their overall dimensions rather than by deepening them. The most favorable structures for cave-level development are two sets of joints conjugated with gently inclined bedding. Since these joints are characterized by a small vertical extent, downward development is limited. Hence, most of the passages are wide but not deep canyons and typical of a water-table cave pattern. Places where the fault plane is eroded from the surface and where, at the same time, an underneath cave chamber ceiling expands upwards are particularly predisposed to the formation of a tiankeng.

  8. Metagenomic Analysis from the Interior of a Speleothem in Tjuv-Ante's Cave, Northern Sweden.

    Directory of Open Access Journals (Sweden)

    Marie Lisandra Zepeda Mendoza

    Full Text Available Speleothems are secondary mineral deposits normally formed by water supersaturated with calcium carbonate percolating into underground caves, and are often associated with low-nutrient and mostly non-phototrophic conditions. Tjuv-Ante's cave is a shallow-depth cave formed by the action of waves, with granite and dolerite as major components, and opal-A and calcite as part of the speleothems, making it a rare kind of cave. We generated two DNA shotgun sequencing metagenomic datasets from the interior of a speleothem from Tjuv-Ante's cave representing areas of old and relatively recent speleothem formation. We used these datasets to perform i an evaluation of the use of these speleothems as past biodiversity archives, ii functional and taxonomic profiling of the speleothem's different formation periods, and iii taxonomic comparison of the metagenomic results to previous microscopic analyses from a nearby speleothem of the same cave. Our analyses confirm the abundance of Actinobacteria and fungi as previously reported by microscopic analyses on this cave, however we also discovered a larger biodiversity. Interestingly, we identified photosynthetic genes, as well as genes related to iron and sulphur metabolism, suggesting the presence of chemoautotrophs. Furthermore, we identified taxa and functions related to biomineralization. However, we could not confidently establish the use of this type of speleothems as biological paleoarchives due to the potential leaching from the outside of the cave and the DNA damage that we propose has been caused by the fungal chemical etching.

  9. Sistema Faro, Isla de Mona, Puerto Rico: speleogenesis of the worlds largest flank margin cave

    International Nuclear Information System (INIS)

    Lace, M. J.; Kambesis, P. N.; Mylroie, J. E.

    2016-01-01

    Isla de Mona, a small, uplifted carbonate plateau jutting out of the waters of the Mona Passage, is an incredibly fragile and densely karstic environment. Expedition work was conducted by the Isla de Mona Project in cooperation with the Departamento Recursos Naturales y Ambientales de Puerto Rico (DRNA), including contributions from many researchers and cavers volunteering from across the U.S and Puerto Rico in the course of 12 separate expeditions, spanning a 14 year period (1998 to 2013). Over 200 caves have been documented on the island to date, the majority of this inventory is composed of flank margin caves but also includes sea caves, pit caves and talus caves. The most extensive example of cave development on the island is Sistema Faro - a sprawling maze-like series of chambers formed within the eastern point of the island with over 40 cliffside entrances overlooking the Caribbean Sea. Detailed cartography and analysis of the geomorphology and development of the Sistema Faro has helped form a complex model of carbonate island cave development as a function of tectonic uplift, lithology, sea level changes, karst hydrogeology and cliff retreat. This communication examines the roles these controls have played in the genesis of the world's largest flank margin cave. (Author)

  10. A review of factors affecting cave climates for hibernating bats in temperate North America

    Science.gov (United States)

    Roger W. Perry

    2013-01-01

    The fungal pathogen Geomyces destructans, which causes white-nose syndrome in bats, thrives in the cold and moist conditions found in caves where bats hibernate. To aid managers and researchers address this disease, an updated and accessible review of cave hibernacula and cave microclimates is presented. To maximize energy savings and reduce...

  11. Eastern Cave- and Crevice-Dwelling Bats Potentially Impacted by USACE Reservoir Operations

    National Research Council Canada - National Science Library

    Martin, C.O

    2002-01-01

    ...; Kasul, Martin, and Allen 2000). This note provides information on six species of forest bats that roost in caves or cave-like structures and potentially occur on Corps projects in the Eastern United States...

  12. Pre-excavation studies of prehistoric cave sites by magnetic prospecting

    Science.gov (United States)

    Itkis., Sonia; Matskevich, Zinovii; Meshveliani, Tengiz

    2014-05-01

    Detailed magnetic survey was performed for caves study in Israel (1995-1996) within the framework of the Beit Shemesh Regional Project (Judean Shephelah). The experience accumulated in Israel we applied later (2010) in two Georgian prehistoric cave sites: Cherula and Kotias-Klde. The magnetic method is based on the contrast in magnetic properties between a target object (e.g., buried archaeological feature) and the host medium (i.e, the surrounding bedrock and soil). The feasibility of the magnetic method for cave revealing was evaluated by magnetic susceptibility (κ) measurements of surrounding soil and rocks, and archaeological features: stones making up the walls, ceramic fragments and cave fill. According to data obtained, the κ of soil within caves (cave fill) is higher than that of surrounding soil. The enhancement of cave fill κ occurs because processes associated with human habitation: repeated heating and accumulation of organic debris. Both these processes provide good conditions for the conversion of the iron oxide found within the soil to a strongly ferromagnetic form (Mullins, 1977; Maher, 1986; Dalan and Banerjee, 1998, Itkis and Eppelbaum, 1999; Itkis, 2003) The presence of highly magnetic ceramics in caves also enhances magnetic contrast between practically non-magnetic bed rock (chalk in Ramat Beit Shemesh Site (Israel) and limestone (Georgian sites) and the cave fill, increasing the potential of the magnetic method to reveal caves (Itkis, 2011). Based on magnetic survey results, an excavation revealed a cave with a large amount of well preserved pottery and finds typical of the Early Bronze Age. Both studied cave sites in Georgia were located in Chiatura region of Imeretia province. Cherula site is a karstic rockshelter with a single chamber, ca 100 sq. m. The site was briefly tested in 1970s'. The area excavated in 2010 went to the depth of 60 cm below the present day surface; the limestone bedrock was not reached. The excavation revealed

  13. 3D Volume and Morphology of Perennial Cave Ice and Related Geomorphological Models at Scăriloara Ice Cave, Romania, from Structure from Motion, Ground Penetrating Radar and Total Station Surveys

    Science.gov (United States)

    Hubbard, J.; Onac, B. P.; Kruse, S.; Forray, F. L.

    2017-12-01

    Research at Scăriloara Ice Cave has proceeded for over 150 years, primarily driven by the presence and paleoclimatic importance of the large perennial ice block and various ice speleothems located within its galleries. Previous observations of the ice block led to rudimentary volume estimates of 70,000 to 120,000 cubic meters (m3), prospectively placing it as one of the world's largest cave ice deposits. The cave morphology and the surface of the ice block are now recreated in a total station survey-validated 3D model, produced using Structure from Motion (SfM) software. With the total station survey and the novel use of ArcGIS tools, the SfM validation process is drastically simplified to produce a scaled, georeferenced, and photo-texturized 3D model of the cave environment with a root-mean-square error (RMSE) of 0.24 m. Furthermore, ground penetrating radar data was collected and spatially oriented with the total station survey to recreate the ice block basal surface and was combined with the SfM model to create a model of the ice block itself. The resulting ice block model has a volume of over 118,000 m3 with an uncertainty of 9.5%, with additional volumes left un-surveyed. The varying elevation of the ice block basal surface model reflect specific features of the cave roof, such as areas of enlargement, shafts, and potential joints, which offer further validation and inform theories on cave and ice genesis. Specifically, a large depression area was identified as a potential area of initial ice growth. Finally, an ice thickness map was produced that will aid in the designing of future ice coring projects. This methodology presents a powerful means to observe and accurately characterize and measure cave and cave ice morphologies with ease and affordability. Results further establish the significance of Scăriloara's ice block to paleoclimate research, provide insights into cave and ice block genesis, and aid future study design.

  14. 76 FR 42654 - Endangered and Threatened Wildlife and Plants; Petition To List Grand Canyon Cave Pseudoscorpion

    Science.gov (United States)

    2011-07-19

    ... from 1977 to 1978. Eight caves were examined including Babylon Cave, Crystal Forest Cave, Land's End...) describes examining the walls, ceilings, and floors for animals and invertebrates. He identified 12...

  15. Hydrogeology of the Sterkfontein Cave System, Cradle of Humankind, South Africa

    CSIR Research Space (South Africa)

    Hobbs, Philip J

    2017-09-01

    Full Text Available A water level rise of almost 3 m in the space of two years in the Sterkfontein Cave system since late-2009 necessitated the re-routing of the tourist path through the cave to successively higher elevations on three occasions. It also raisedconcern...

  16. The Astrobiology of the Subsurface: Exploring Cave Habitats on Earth, Mars and Beyond

    Science.gov (United States)

    Boston, Penelope Jane

    2016-01-01

    We are using the spectacular underground landscapes of Earth caves as models for the subsurfaces of other planets. Caves have been detected on the Moon and Mars and are strongly suspected for other bodies in the Solar System including some of the ice covered Ocean Worlds that orbit gas giant planets. The caves we explore and study include many extreme conditions of relevance to planetary astrobiology exploration including high and low temperatures, gas atmospheres poisonous to humans but where exotic microbes can flourish, highly acidic or salty fluids, heavy metals, and high background radiation levels. Some cave microorganisms eat their way through bedrock, some live in battery acid conditions, some produce unusual biominerals and rare cave formations, and many produce compounds of potential pharmaceutical and industrial significance. We study these unique lifeforms and the physical and chemical biosignatures that they leave behind. Such traces can be used to provide a "Field Guide to Unknown Organisms" for developing life detection space missions.

  17. Potential collapse due to geological structures influence in Seropan Cave, Gunung Kidul, Yogyakarta, Indonesia

    Science.gov (United States)

    Nugroho, B.; Pranantya, P. A.; Witjahjati, R.; Rofinus

    2018-01-01

    This study aims to estimate the potential collapse in the Seropan cave, based on the existing geological structure conditions in the cave. This is very necessary because in the Seropan cave will be built Microhydro installation for power plants. The electricity will be used to raise the underground river water in the cave to a barren soil surface, which can be used for surface irrigation. The method used is analysis the quality of rock mass along the cave. Analysis of rock mass quality using Geomechanical Classification or Rock Mass Rating (RMR), to determine the magnitude of the effect of geological structure on rock mass stability. The research path is divided into several sections and quality analysis is performed on each section. The results show that the influence of geological structure is very large and along the cave where the research there are several places that have the potential to collapse, so need to get serious attention in handling it. Nevertheless, the construction of this Microhydro installation can still be carried out by making a reinforcement on potentially collapsing parts

  18. Diversity of cultivable bacteria involved in the formation of macroscopic microbial colonies (Cave silver on the walls of a cave in Slovenia

    Directory of Open Access Journals (Sweden)

    Blagajana Herzog Velikonja

    2014-01-01

    Full Text Available Karstic caves often support white, yellow, grey or pink microbial colonies that are termed ‘cave silver’ by speleologists. Using various sample pre-treatments and culture media, a wide variety of bacteria associated with these colonies were recovered from a cave in Slovenia, Pajsarjeva jama. Decreasing the inoculum size resulted in significant increases in viable counts, while pre-treatments had the opposite effect with the exception of microwave irradiation. While all growth media yielded viable counts, the maximal counts were observed on a low-nutrient TWA medium. Based on the 16S rRNA gene sequence of OTU representatives, the majority of the 80 isolates examined belonged to Streptomyces (25%, Micrococcus (16% and Rhodococcus (10% Other abundant groups were Pseudomonas (9%, Agrobacterium (8%, Lysobacter (6% and Paenibacillus (5%, while members of genera Microbacterium, Agrococcus, Arthrobacter, Bacillus, Kocuria, Oerskovia, Sphingomonas, Aerococcus, and Bosea represented a minor portion of cultivable diversity encountered. Members of Streptomyces and Agrobacterium were common to all samples. Although these microorganisms readily form colonies under laboratory conditions, they were unrelated to abundant environmental phylotypes recovered from same samples in a previous study. However, the comparative 16S rRNA analysis showed that microorganisms highly related to the ones obtained in this study were cultivated from other subterranean environments indicating that they might represent true microbial cave dwellers.

  19. Hotspot mitigation in the StarCAVE

    KAUST Repository

    Rhee, Jordan; Schulze, Jurgen; DeFanti, Thomas A.

    2010-01-01

    Rear-projected screens such as those in Digital Light Projection (DLP) televisions suffer from an image quality problem called hot spotting, where the image is brightest at a point dependent on the viewing angle. In rear-projected mulit-screen configurations such as the StarCAVE at Calit2, this causes discontinuities in brightness at the edges where screens meet, and thus in the 3D image perceived by the user. In the StarCAVE we know the viewer's position in 3D space and we have programmable graphics hardware, so we can mitigate this effect by performing post-processing in the inverse of the pattern, yielding a homogenous image at the output. Our implementation improves brightness homogeneity by a factor of 4 while decreasing frame rate by only 1-3 fps.

  20. Hotspot mitigation in the StarCAVE

    KAUST Repository

    Rhee, Jordan

    2010-01-27

    Rear-projected screens such as those in Digital Light Projection (DLP) televisions suffer from an image quality problem called hot spotting, where the image is brightest at a point dependent on the viewing angle. In rear-projected mulit-screen configurations such as the StarCAVE at Calit2, this causes discontinuities in brightness at the edges where screens meet, and thus in the 3D image perceived by the user. In the StarCAVE we know the viewer\\'s position in 3D space and we have programmable graphics hardware, so we can mitigate this effect by performing post-processing in the inverse of the pattern, yielding a homogenous image at the output. Our implementation improves brightness homogeneity by a factor of 4 while decreasing frame rate by only 1-3 fps.

  1. 36 CFR 290.3 - Nomination, evaluation, and designation of significant caves.

    Science.gov (United States)

    2010-07-01

    ..., DEPARTMENT OF AGRICULTURE CAVE RESOURCES MANAGEMENT § 290.3 Nomination, evaluation, and designation of... on National Forest System lands shall possess one or more of the following features, characteristics... information. (4) Hydrologic. The cave is a part of a hydrologic system or contains water which is important to...

  2. Report of a three-year monitoring programme at Heshang Cave, Central China

    Directory of Open Access Journals (Sweden)

    Chaoyong Hu

    2008-10-01

    Full Text Available Heshang Cave is situated in central China (30º27’N, 110º25’E; 294 m in the middle reaches of the Yangtze Valley, a region stronglyimpacted by the East Asian Monsoon. It contains large annually-laminated Holocene and late Pleistocene stalagmites which capture pastmonsoon behaviour with seasonal resolution, and could enhance understanding of the amplitude and frequency of monsoon behaviour indifferent climate states. In this paper, we present results of a 3-year monitoring programme at Heshang. T loggers outside the cave agree closely with T data from nearby meteorological stations. T at the site of growth of the largest recovered stalagmite averages 18ºC (identical to mean annual T outside the cave with a seasonal amplitude of 5ºC (about one fifth of the external cycle. Rainfall measurements from a station 3 km from the cave indicate strong summer monsoon rain in 2004 and 2005, but rather weaker summer rain (by ≈30% in 2006.Drip rate at the monitoring site has a base flow of 14 drips/minute and shows a sharp increase to ≈40 drips/minute early in the summerrains of 2004 and 2005, followed by a gradual return to base-flow as the monsoon weakens. This abrupt change presumably representsthreshold behaviour in the hydrological system. This threshold is not passed in 2006 and there is no abrupt increase in drip rate, indicating the sensitivity of this site (and presumably of speleothem chemistry in this cave to monsoon rainfall. Results are also reported from a 10-month deployment of a Stalagmate drip counter, and for CO2 levels in Heshang Cave. Overall, this monitoring work represents an essential dataset for interpretation of the chemistry of drip waters, of carbonates grown on glass slides and, ultimately, of long speleothem records of past climate from Heshang Cave.

  3. Monitoring of radon gas in caves of the Yorkshire Dales, United Kingdom

    International Nuclear Information System (INIS)

    Langridge, D; Stokes, R P; Jackson, C P

    2010-01-01

    A number of vocational training courses are held in caves in the Yorkshire Dales region of the United Kingdom. The instructors and students involved in these courses have the potential to be exposed to enhanced levels of radon ( 222 Rn) and its progeny as a result of their occupations. A prior radiological risk assessment for the training courses recommended that an environmental monitoring programme be carried out to establish the radon concentrations in the caves, and that the caving instructors wear personal radon dosemeters. Radon gas concentrations varied seasonally, being at their highest in summer and their lowest in winter. The lowest result was 40 Bq m -3 recorded in Lower Longchurn cave during winter, whilst the highest result was 4440 Bq m -3 recorded in Crackpot cave during the summer. As the individuals involved in the caving are entering atmospheres with radon gas concentrations in excess of 400 Bq m -3 , the Ionising Radiation Regulations 1999 (GB Parliament 2000 Ionising Radiations Regulations 1999 (London: Stationary Office) SI 1999/3232) apply. A system of work is therefore in place to control exposure to radon. This system of work stipulates an initial dose investigation level of 1 mSv, a second dose investigation level of 2 mSv and an annual dose limit of 6 mSv. The highest annual dose recorded to date is 2.2 mSv, although the average (median) annual dose is only 0.5 mSv.

  4. Meckel's cave access: anatomic study comparing the endoscopic transantral and endonasal approaches.

    Science.gov (United States)

    Van Rompaey, Jason; Suruliraj, Anand; Carrau, Ricardo; Panizza, Benedict; Solares, C Arturo

    2014-04-01

    Recent advances in endonasal endoscopy have facilitated the surgical access to the lateral skull base including areas such as Meckel's cave. This approach has been well documented, however, few studies have outlined transantral specific access to Meckel's. A transantral approach provides a direct pathway to this region obviating the need for extensive endonasal and transsphenoidal resection. Our aim in this study is to compare the anatomical perspectives obtained in endonasal and transantral approaches. We prepared 14 cadaveric specimens with intravascular injections of colored latex. Eight cadavers underwent endoscopic endonasal transpterygoid approaches to Meckel's cave. Six additional specimens underwent an endoscopic transantral approach to the same region. Photographic evidence was obtained for review. 30 CT scans were analyzed to measure comparative distances to Meckel's cave for both approaches. The endoscopic approaches provided a direct access to the anterior and inferior portions of Meckel's cave. However, the transantral approach required shorter instrumentation, and did not require clearing of the endonasal corridor. This approach gave an anterior view of Meckel's cave making posterior dissection more difficult. A transantral approach to Meckel's cave provides access similar to the endonasal approach with minimal invasiveness. Some of the morbidity associated with extensive endonasal resection could possibly be avoided. Better understanding of the complex skull base anatomy, from different perspectives, helps to improve current endoscopic skull base surgery and to develop new alternatives, consequently, leading to improvements in safety and efficacy.

  5. Guanophilic fungi in three caves of southwestern Puerto Rico

    Directory of Open Access Journals (Sweden)

    Nieves-Rivera Angel M.

    2009-01-01

    Full Text Available Fifty species of guanophilic (bat guano-loving fungi were isolated from field-collected samples within three caves in southwesternPuerto Rico; most were mitosporic fungi (23 species. The caves studied were Cueva La Tuna (Cabo Rojo, Cueva de Malano(Sistema de Los Chorros, San Germán, and Cueva Viento (El Convento Cave-Spring System, Guayanilla-Peñuelas. The mostconspicuous fungus by far was the zygomycete Circinella umbellata (Mucorales. Circinella umbellata dominated the bat guanoincubation chambers (Petri dishes lined with sterile filter paper moistened with sterile water at ambient laboratory conditions.Nineteen species of basidiomycetes (e.g., Ganoderma cf. resinaceum, Geastrum cf. minimum, Lepiota sp., Polyporus sp., Ramariasp. and three species of ascomycetes (Hypoxylon sp., Xylaria anisopleura, and X. kegeliana were also recorded. They were foundon soil, rotting leaves, bark and rotting wood, buried in bat guano located below natural skylights or sinkholes.

  6. Geological conditions of origin of the Potočka zijalka cave

    Directory of Open Access Journals (Sweden)

    Stanko Buser

    2002-12-01

    Full Text Available In the Potočka zijalka cave on Mt. Ol{eva in Southern Karavanke during excavation of cave sediments of Würm age that contain cultural remains of the Cromagnon Man also large amounts of gravel were before the Badenian transgression, from the Central Alps during Miocene.

  7. A Framework for Aligning Instructional Design Strategies with Affordances of CAVE Immersive Virtual Reality Systems

    Science.gov (United States)

    Ritz, Leah T.; Buss, Alan R.

    2016-01-01

    Increasing availability of immersive virtual reality (IVR) systems, such as the Cave Automatic Virtual Environment (CAVE) and head-mounted displays, for use in education contexts is providing new opportunities and challenges for instructional designers. By highlighting the affordances of IVR specific to the CAVE, the authors emphasize the…

  8. A Transcriptomic Analysis of Cave, Surface, and Hybrid Isopod Crustaceans of the Species Asellus aquaticus.

    Directory of Open Access Journals (Sweden)

    Bethany A Stahl

    Full Text Available Cave animals, compared to surface-dwelling relatives, tend to have reduced eyes and pigment, longer appendages, and enhanced mechanosensory structures. Pressing questions include how certain cave-related traits are gained and lost, and if they originate through the same or different genetic programs in independent lineages. An excellent system for exploring these questions is the isopod, Asellus aquaticus. This species includes multiple cave and surface populations that have numerous morphological differences between them. A key feature is that hybrids between cave and surface individuals are viable, which enables genetic crosses and linkage analyses. Here, we advance this system by analyzing single animal transcriptomes of Asellus aquaticus. We use high throughput sequencing of non-normalized cDNA derived from the head of a surface-dwelling male, the head of a cave-dwelling male, the head of a hybrid male (produced by crossing a surface individual with a cave individual, and a pooled sample of surface embryos and hatchlings. Assembling reads from surface and cave head RNA pools yielded an integrated transcriptome comprised of 23,984 contigs. Using this integrated assembly as a reference transcriptome, we aligned reads from surface-, cave- and hybrid- head tissue and pooled surface embryos and hatchlings. Our approach identified 742 SNPs and placed four new candidate genes to an existing linkage map for A. aquaticus. In addition, we examined SNPs for allele-specific expression differences in the hybrid individual. All of these resources will facilitate identification of genes and associated changes responsible for cave adaptation in A. aquaticus and, in concert with analyses of other species, will inform our understanding of the evolutionary processes accompanying adaptation to the subterranean environment.

  9. Fossil remains of the cave animal Marifugia cavatica in the unroofed cave in the Črnotiče quarry, W Slovenia

    Directory of Open Access Journals (Sweden)

    Andrej Mihevc

    2002-12-01

    Full Text Available The paper is a report on the discovery of tubes of a fossilised cave serpulid in a roofless cave in the quarry near village Črnotiče. The site and shape of the fossilised tubes are described. The animals lived attached to the scallops in the wall of the water filled passage. The passage was later filled with clay deposits, followed by a layer of sinter several metres thick. The roof of the passage has been removed by karst denudation and the sinter now reaches to the surface.Fragments of tubes of animals of various sizes have been preserved, attached to the rock wall; those tubes that grew at a right angle to the wall have broken off but been preserved in the sediment. In terms of their dimension and shape the tubes remind one of the tubes of the more recent cave serpulid Marifugia cavatica Absolon and Hrabe. Thedating of the nearby sediments by palaeomagnetic method show that the minimum age of the serpulids is 1.7 Ma, but greater age is more probable.

  10. Phylogenetic diversity of fungal communities in areas accessible and not accessible to tourists in Naracoorte Caves.

    Science.gov (United States)

    Adetutu, Eric M; Thorpe, Krystal; Bourne, Steven; Cao, Xiangsheng; Shahsavari, Esmaeil; Kirby, Greg; Ball, Andrew S

    2011-01-01

    The fungal diversity in areas accessible and not accessible to tourists at UNESCO World Heritage-listed Naracoorte Caves was investigated with culture-dependent and culture-independent techniques for assistance in cave management protocol development. The caves were selected based on tourist numbers and configurations: Stick Tomato (open, high numbers), Alexandra (lockable openings, high numbers) and Strawhaven (control; no access). Culture-based survey revealed Ascomycota dominance irrespective of sampling area with Microascales (Trichurus sp.) being most frequently isolated. Some Hypocreales-like sequences belonging to Fusarium sp., Trichoderma sp. and Neonectria sp. (Stick Tomato) were cultured only from areas not accessible to tourists. These orders also were detected by DGGE assay irrespective of sampling area. The predominance of Ascomycota (especially Microascales) suggested their important ecological roles in these caves. Culture-independent analysis showed higher Shannon fungal diversity values (from ITS-based DGGE profiles) in tourist-accessible areas of these caves than in inaccessible areas with the fungal community banding patterns being substantially different in Stick Tomato Cave. Further investigations are needed to determine the cause of the differences in the fungal communities of Stick Tomato Cave, although cave-related factors such as use, configuration and sediment heterogeneity might have contributed to these differences.

  11. A cave population of Isbrueckerichthys alipionis (Gosline, 1947 in the Upper Ribeira karst area, southeastern Brazil (Siluriformes: Loricariidae

    Directory of Open Access Journals (Sweden)

    Eleonora Trajano

    Full Text Available A cave population of the armored catfish Isbrueckerichthys alipionis is reported from the Santana Cave, in the rio Betari watershed, Upper Ribeira karst area, Iporanga, São Paulo State, southeastern Brazil. The cave population was compared to an epigean population of I. alipionis and no significant differences where found in morphology or degree of pigmentation. As the cave population is known for at least 30 years and is apparently isolated from epigean streams, it is classified as troglophilic. The discovery of this troglophilic species in the Santana Cave is an additional strong argument for the conservation of that cave.

  12. Effect of soil-rock system on speleothems weathering in Bailong Cave, Yunnan Province, China*

    Science.gov (United States)

    Wang, Jing; Song, Lin-hua

    2005-01-01

    Bailong Cave with its well-developed Middle Triassic calcareous dolomite’s system was opened as a show cave for visitors in 1988. The speleothem scenery has been strongly weathered as white powder on the outer layers. Study of the cave winds, permeability of soil-rock system and the chemical compositions of the dripping water indicated: (1) The cave dimension structure distinctively affects the cave winds, which were stronger at narrow places. (2) Based on the different soil grain size distribution, clay was the highest in composition in the soil. The response sense of dripping water to the rainwater percolation was slow. The density of joints and other openings in dolomite make the dolomite as mesh seepage body forming piles of thin and high columns and stalactites. (3) Study of 9 dripping water samples by HYDROWIN computer program showed that the major mineral in the water was dolomite. PMID:15682505

  13. Aspergillus baeticus sp. nov. and Aspergillus thesauricus sp. nov., two species in section Usti from Spanish caves.

    Science.gov (United States)

    Nováková, Alena; Hubka, Vit; Saiz-Jimenez, Cesareo; Kolarik, Miroslav

    2012-11-01

    Two novel species of Aspergillus that are clearly distinct from all known species in section Usti were revealed during a study of microfungal communities in Spanish caves. The novel species identified in this study and additional species of Aspergillus section Usti are associated with places and substrates related to human activities in caves. Novel species are described using data from four loci (ITS, benA, caM and rpb2), morphology and basic chemical and physiological analyses. Members of the species Aspergillus thesauricus sp. nov. were isolated from various substrates, including decaying organic matter, cave air and cave sediment of the Cueva del Tesoro Cave (the Treasure cave); the species is represented by twelve isolates and is most closely related to the recently described Aspergillus germanicus. Members of the species Aspergillus baeticus sp. nov. were isolated from cave sediment in the Gruta de las Maravillas Cave (the Grotto of the Marvels); the species is represented by two isolates. An additional isolate was found in the Cueva del Tesoro Cave and in the Demänovská Peace Cave (Slovakia), suggesting a potentially wide distribution of this micro-organism. The species is related to Aspergillus ustus and Aspergillus pseudoustus. Both species were unable to grow at 37 °C, and a weakly positive, light greenish yellow Ehrlich reaction was observed in A. thesauricus. Unique morphological features alone are sufficient to distinguish both species from related taxa.

  14. Cova des Pas de Vallgornera, (Mallorca, Spain: history of exploration and cave description

    Directory of Open Access Journals (Sweden)

    Antoni Merino

    2014-05-01

    Full Text Available The Cova des Pas de Vallgornera lies in the Llucmajor municipality, in southern Mallorca, and is the longest cave in the Balearic Islands. Currently its surveyed length is over 74,000 metres, including more than 17,000 of underwater extensions. The cave was discovered accidentally in 1968, but it was in 2004 when a major breakthrough shed light on its real extension and importance. The cave roughly shows two tiers of passages, apart from the underwater extensions, the first one is between 7 and 11 m above the mean sea level, the second one is about at the water table level. The importance of the cave is not only related to its extension, but also to the presence of a wide variety of speleothems and outstanding solutional morphologies that evidence a complex evolution. The cave is under the protection of Conselleria de Medi Ambient, Govern de les Illes Balears (the Regional Environmental Authority and was declared Site of Community Importance, within the Natura 2000 Network

  15. Climatic control on the growth of gigantic gypsum crystals within hypogenic caves (Naica mine, Mexico)?

    Science.gov (United States)

    Garofalo, Paolo S.; Fricker, Mattias B.; Günther, Detlef; Forti, Paolo; Mercuri, Anna-Maria; Loreti, Mara; Capaccioni, Bruno

    2010-01-01

    Three hypogenic caves within the Naica mine of Mexico ( Cueva de los Cristales — CLC, Ojo de la Reina — OR, and Cueva de las Velas — CLV) host spectacular gypsum crystals up to 11 m in length. These caves are close to another shallow cave of the area ( Cueva de las Espadas — CLE), with which they cover a 160 m-deep vertical section of the local drainage basin. Similar to other hypogenic caves, all these caves lack a direct connection with the land surface and should be unrelated with climate. A record of multi-technique fluid inclusion data and pollen spectra from cave and mine gypsum indicates surprisingly that climatic changes occurring at Naica could have controlled fluid composition in these caves, and hence crystal growth. Microthermometry and LA-ICP-Mass Spectrometry of fluid inclusions indicate that the shallow, chemically peculiar, saline fluid (up to 7.7 eq. wt.%NaCl) of CLE could have formed from evaporation, during a dry and hot climatic period. The fluid of the deep caves was instead of low salinity (˜ 3.5 eq. wt.% NaCl) and chemically homogeneous, and was poorly affected by evaporation. We propose that mixing of these two fluids, generated at different depths of the Naica drainage basin, determined the stable supersaturation conditions for the gigantic gypsum crystals to grow. Fluid mixing was controlled by the hydraulic communication between CLE and the other deep caves, and must have taken place during cycles of warm-dry and fresh-wet climatic periods, which are known to have occurred in the region. Pollen grains from a 35 ka-old gypsum crystal of CLC corresponds to a fairly homogenous catchment basin made of a mixed broadleaf wet forest, which suggests precipitation during a fresh-wet climatic period and confirms our interpretation of the fluid inclusion data. The unusual combination of geological and geochemical factors of Naica suggests that other hypogenic caves found elsewhere may not host similar crystals. However, this work shows that

  16. Reassessing Coxcatlan Cave and the early history of domesticated plants in Mesoamerica.

    Science.gov (United States)

    Smith, Bruce D

    2005-07-05

    Reanalysis and direct accelerator mass spectrometry radiocarbon dating of the cucurbit assemblage from Coxcatlan Cave provide information on the timing and sequence of the initial appearance of three domesticated plants in the Tehuacán Valley (Puebla, Mexico) and allow reassessment of the overall temporal context of plant domestication in Mexico. Cucurbita pepo is the earliest documented domesticate in the cave, dating to 7,920 calibrated calendrical (cal) years B.P. The bottle gourd (Lagenaria siceraria) is dated at 7,200 cal years B.P. Cucurbita argyrosperma does not appear until 2,065 cal years B.P. The earlier identification of Cucurbita moschata specimens is not confirmed. Seventy-one radiocarbon dates, including 23 accelerator mass spectrometry dates on cucurbits, provide ample evidence of postdepositional vertical displacement of organic materials in the western half of Coxcatlan Cave, but they also indicate that the eastern half of the cave was largely undisturbed.

  17. “Spray Technique: Tracing the Sketch Traditions of Limestone Cave in Lenggong, Perak”

    Directory of Open Access Journals (Sweden)

    Yahaya Fatan Hamamah

    2015-01-01

    Full Text Available Archaeological research provides the widest opportunity for researchers to analyse various aspects and disciplines appropriate to the subject and the object of choice. Subject and object selection is the work of exploration artefacts found in particular sites and archaeological heritage. Exploration and excavation on of a world heritage site such as Lenggong enables researchers to uncover various archaeological artefacts that are rich and meaningful. To find evidence of the strength and benefits of an artefact, further studies on each artefact should be carried out continuously. This essay will track the wisdom of the ancient artists use to produce paintings in a limestone cave in Lenggong, Perak, using spray techniques. Some artefacts that are identified as cave paintings show a very interesting sketch technique that are unique and special. This essay will also examine some of the cave paintings in other caves in Perak and also other caves in several countries as comparison. Studies involving cave paintings in Malaysia are new compared to Western countries. Thus, the study of one of the technique which is spray technique can open the eyes of the audience to acknowledge and recognise the ancient heritage. It also hoped that this study is able to increase the body of knowledge that goes beyond the boundaries of the arts district and the country.

  18. The Evaluation of a Motion Base Driving Simulator in a Cave at TACOM

    National Research Council Canada - National Science Library

    Mollenhauer, M. A; Romano, R. A; Brumm, B

    2004-01-01

    The purpose of this presentation is to describe the highlights of a research program designed to investigate the feasibility of creating a motion base driving simulator in a Cave Automatic Virtual Environment (CAVE...

  19. Occupational exposure to radon in Australian Tourist Caves an Australian-wide study of radon levels

    International Nuclear Information System (INIS)

    Solomon, S.B.; Langroo, R.; Peggie, J.R.; Lyons, R.G.; James, J.M.

    1996-02-01

    The study described in this report sets out to determine which Australian show caves have long- term radon levels in excess of the proposed action level of 1000 Bq m -3 . The collaborative study between the Australian Radiation Laboratory (ARL), the University of Sydney and the University of Auckland, was carried out with the support of a Research Grant from Worksafe Australia. The aims of this study were to measure radon levels for each season over a period of one year, at representative sites in all developed show caves around Australia, to determine yearly average radon levels for each cave tour, based on these site measurements, to estimate the radiation doses to the tour guides employed in these caves, and to identify caves with radon concentrations in excess of the action level. (authors)

  20. Estimation of deep infiltration in unsaturated limestone environments using cave lidar and drip count data

    Science.gov (United States)

    Mahmud, K.; Mariethoz, G.; Baker, A.; Treble, P. C.; Markowska, M.; McGuire, E.

    2016-01-01

    Limestone aeolianites constitute karstic aquifers covering much of the western and southern Australian coastal fringe. They are a key groundwater resource for a range of industries such as winery and tourism, and provide important ecosystem services such as habitat for stygofauna. Moreover, recharge estimation is important for understanding the water cycle, for contaminant transport, for water management, and for stalagmite-based paleoclimate reconstructions. Caves offer a natural inception point to observe both the long-term groundwater recharge and the preferential movement of water through the unsaturated zone of such limestone. With the availability of automated drip rate logging systems and remote sensing techniques, it is now possible to deploy the combination of these methods for larger-scale studies of infiltration processes within a cave. In this study, we utilize a spatial survey of automated cave drip monitoring in two large chambers of Golgotha Cave, south-western Western Australia (SWWA), with the aim of better understanding infiltration water movement and the relationship between infiltration, stalactite morphology, and unsaturated zone recharge. By applying morphological analysis of ceiling features from Terrestrial LiDAR (T-LiDAR) data, coupled with drip time series and climate data from 2012 to 2014, we demonstrate the nature of the relationships between infiltration through fractures in the limestone and unsaturated zone recharge. Similarities between drip rate time series are interpreted in terms of flow patterns, cave chamber morphology, and lithology. Moreover, we develop a new technique to estimate recharge in large-scale caves, engaging flow classification to determine the cave ceiling area covered by each flow category and drip data for the entire observation period, to calculate the total volume of cave discharge. This new technique can be applied to other cave sites to identify highly focussed areas of recharge and can help to better

  1. Experimental study to evaluate the pathogenicity of Streptococcus iniae in Guppy (Poecilia reticulata

    Directory of Open Access Journals (Sweden)

    Milad Adel

    2015-01-01

    Full Text Available Streptococcus iniae has emerged as an important fish pathogen over the last decade in farmed rainbow trout in Iran. The main objective of this study was to evaluate the pathogenicity of S. iniae in Poecilia reticulata. Atotal of 60 apparently healthy P. reticulata were obtained from ornamental fish pet store and injected intraperitoneally with 1.5×106 cfu of bacteria. For 14 days after challenge, the rate of mortality and clinical signs were recorded. The first clinical signs was observed in challenged fish 48 hrs after injection of S. iniae and first mortality was observed 72 hrs after injection. No significant differences in mortality and clinical signs between both sexes were observed. Streptococcus iniae was collected from internal organs of fishes challenged, and was confirmed using the conventional biochemical tests and PCR. It is concluded that, P. reticulata is susceptible to streptococcosis and can play an important role in transmission of the disease to other ornamental fish species and also cultured fish.

  2. The Astrobiology of the Subsurface: Caves and Rock Fracture Habitats on Earth, Mars and Beyond

    Science.gov (United States)

    Boston, Penelope J.

    2017-01-01

    The Astrobiology of the Subsurface: Exploring Cave Habitats on Earth, Mars and Beyond. We are using the spectacular underground landscapes of Earth caves as models for the subsurfaces of other planets. Caves have been detected on the Moon and Mars and are strongly suspected for other bodies in the Solar System including some of the ice covered Ocean Worlds that orbit gas giant planets. The caves we explore and study include many extreme conditions of relevance to planetary astrobiology exploration including high and low temperatures, gas atmospheres poisonous to humans but where exotic microbes can fluorish, highly acidic or salty fluids, heavy metals, and high background radiation levels. Some cave microorganisms eat their way through bedrock, some live in battery acid conditions, some produce unusual biominerals and rare cave formations, and many produce compounds of potential pharmaceutical and industrial significance. We study these unique lifeforms and the physical and chemical biosignatures that they leave behind. Such traces can be used to provide a Field Guide to Unknown Organisms for developing life detection space missions.

  3. Poecilia picta, a Close Relative to the Guppy, Exhibits Red Male Coloration Polymorphism: A System for Phylogenetic Comparisons.

    Directory of Open Access Journals (Sweden)

    Anna K Lindholm

    Full Text Available Studies on the evolution of female preference and male color polymorphism frequently focus on single species since traits and preferences are thought to co-evolve. The guppy, Poecilia reticulata, has long been a premier model for such studies because female preferences and orange coloration are well known to covary, especially in upstream/downstream pairs of populations. However, focused single species studies lack the explanatory power of the comparative method, which requires detailed knowledge of multiple species with known evolutionary relationships. Here we describe a red color polymorphism in Poecilia picta, a close relative to guppies. We show that this polymorphism is restricted to males and is maintained in natural populations of mainland South America. Using tests of female preference we show female P. picta are not more attracted to red males, despite preferences for red/orange in closely related species, such as P. reticulata and P. parae. Male color patterns in these closely related species are different from P. picta in that they occur in discrete patches and are frequently Y chromosome-linked. P. reticulata have an almost infinite number of male patterns, while P. parae males occur in discrete morphs. We show the red male polymorphism in P. picta extends continuously throughout the body and is not a Y-linked trait despite the theoretical prediction that sexually-selected characters should often be linked to the heterogametic sex chromosome. The presence/absence of red male coloration of P. picta described here makes this an ideal system for phylogenetic comparisons that could reveal the evolutionary forces maintaining mate choice and color polymorphisms in this speciose group.

  4. Technical devices of powered roof support for the top coal caving as automation objects

    Science.gov (United States)

    Nikitenko, M. S.; Kizilov, S. A.; Nikolaev, P. I.; Kuznetsov, I. S.

    2018-05-01

    In the paper technical devices for the top coal caving as automation objects in the composition of the longwall mining complex (LTCC) are considered. The proposed concept for automation of the top coal caving process allows caving efficiency to be ensured, coal dilution to be prevented, conveyor overloading to be prevented, the shearer service personnel to be unloaded, the influence of the “human factor” to be reduced.

  5. Marine Caves of the Mediterranean Sea: A Sponge Biodiversity Reservoir within a Biodiversity Hotspot

    Science.gov (United States)

    Gerovasileiou, Vasilis; Voultsiadou, Eleni

    2012-01-01

    Marine caves are widely acknowledged for their unique biodiversity and constitute a typical feature of the Mediterranean coastline. Herein an attempt was made to evaluate the ecological significance of this particular ecosystem in the Mediterranean Sea, which is considered a biodiversity hotspot. This was accomplished by using Porifera, which dominate the rocky sublittoral substrata, as a reference group in a meta-analytical approach, combining primary research data from the Aegean Sea (eastern Mediterranean) with data derived from the literature. In total 311 species from all poriferan classes were recorded, representing 45.7% of the Mediterranean Porifera. Demospongiae and Homoscleromorpha are highly represented in marine caves at the family (88%), generic (70%), and species level (47.5%), the latter being the most favored group along with Dictyoceratida and Lithistida. Several rare and cave-exclusive species were reported from only one or few caves, indicating the fragmentation and peculiarity of this unique ecosystem. Species richness and phylogenetic diversity varied among Mediterranean areas; the former was positively correlated with research effort, being higher in the northern Mediterranean, while the latter was generally higher in caves than in the overall sponge assemblages of each area. Resemblance analysis among areas revealed that cavernicolous sponge assemblages followed a pattern quite similar to that of the overall Mediterranean assemblages. The same pattern was exhibited by the zoogeographic affinities of cave sponges: species with Atlanto-Mediterranean distribution and Mediterranean endemics prevailed (more than 40% each), 70% of them having warm-water affinities, since most caves were studied in shallow waters. According to our findings, Mediterranean marine caves appear to be important sponge biodiversity reservoirs of high representativeness and great scientific interest, deserving further detailed study and protection. PMID:22808070

  6. Sensing Structures Inspired by Blind Cave Fish

    Science.gov (United States)

    McConney, Michael E.; Chen, Nannan; Lu, David; Anderson, Kyle D.; Hu, Huan; Liu, Chang; Tsukruk, Vladimir V.

    2009-03-01

    Blind cave fish, with degenerated non-functioning eyes, have evolved to ``see'' their hydrodynamic environment by using the flow receptors of the lateral line system. The hair-cell receptors are encapsulated in a hydrogel-like material, called a cupula, which increases the sensitivity of the hair-cell receptors by coupling their motion to the surrounding flowing media. We characterized the viscoelastic properties and of blind cave fish cupulae by using colloidal-probe spectroscopy in fluid. A photo-patternable hydrogel with similar properties was developed to mimic the fish receptor coupling structure. Flow-based measurements indicated that the hydrogels enhance drag through increased surface area, but also inherent material properties. These bio-inspired structures endowed micro-fabricated flow sensors with sensitivities rivaling that of fish.

  7. Incidence of chromosomal aberrations and micronuclei in cave tour guides.

    Science.gov (United States)

    Bilban, M; Bilban-Jakopin, C; Vrhovec, S

    2001-01-01

    An analysis of structural chromosomal aberrations (SCA) and micronucleus tests (MN) were performed in 38 subjects, cave tour guides and in appropriate control group. The dominant type of chromosomal aberrations in tourist guides were chromosomal breaks (0.013 per cell) and acentric fragments (0.011 per cell). In the control group, these aberrations were present up to 0.008 on cells. Considering the analysed cells of the guides in total (33,556), the incidence of dicentric and rings range is below 0.0008 on cells, even though three dicentric and ring chromosoms were found already in the first 1000 in vitro metaphases of some guides. Only 0.0003 dicentrics and neither other translocations were found in control group (ambiental exposure). The incidence of micronuclei in cytokinesis blocked lymphocytes ranged from 12-32 per 500 CB cells in the cave tour guides and from 4-11 per 500 CB cells in control group. Measurements of radon and its daughters were performed at different locations in the cave. Annual doses from 40-60 mSv were estimated per 2000 work hours for cave guides. The changes found in the genome of somatic cells may be related to the exposure doses of radon and its daughters, although smoking should not be ignored.

  8. Clinical Symptoms and Histological Changes in Poecilia reticulata following Gamma-Ray Irradiation

    Directory of Open Access Journals (Sweden)

    K. Beňová

    2006-01-01

    Full Text Available Lethality, food intake, clinical symptoms and terminal histological changes were followed after gamma-irradiation with doses of 10, 20, 30, 35 and 40 Gy in guppy, Poecilia reticulata. Clinical symptoms, food intake and longevity were also monitored in the progeny of fish irradiated with a dose of 10 Gy. In the first days after irradiation timidity and lethargy were observed. After doses of 30, 35 and 40 Gy, these symptoms were accompanied with anorexia. The most prominent clinical symptoms observed were emaciation, hampered breathing, exophthalmia and haemorrhages. Histological findings corresponded with these symptoms. In the fish irradiated with 10 or 20 Gy the progeny survived, after a dose of 30 Gy the progeny died within 24 hours after birth and after doses of 35 and 40 Gy dead progeny was born. The survival data provide an estimate of LD50/30 equal to 29 Gy. Higher relative mortality and more severe clinical symptoms were observed in females. The progeny of irradiated parent fish grew and survived for 3 months maximum.

  9. Cave Conservation Priority Index to Adopt a Rapid Protection Strategy: A Case Study in Brazilian Atlantic Rain Forest

    Science.gov (United States)

    Souza Silva, Marconi; Martins, Rogério Parentoni; Ferreira, Rodrigo Lopes

    2015-02-01

    Cave environments are characterized by possessing specialized fauna living in high environmental stability with limited food conditions. These fauna are highly vulnerable to impacts, because this condition can frequently be easily altered. Moreover, environmental determinants of the biodiversity patterns of caves remain poorly understood and protected. Therefore, the main goal of this work is to propose a cave conservation priority index (CCPi) for a rapid assessment for troglobiotic and troglophile protection. Furthermore, the troglobiotic diversity, distribution and threats have been mapped in the Brazilian Atlantic forest. To propose the CCPi, the human impacts and richness of troglobiotic and troglophile species of 100 caves were associated. Data related to troglomorphic/troglobiotic fauna from another 200 caves were used to map the troglobiotic diversity and distribution. The CCPi reveals extremely high conservation priority for 15 % of the caves, high for 36 % and average for 46 % of the caves. Fourteen caves with extremely high priorities should have urgent conservation and management actions. The geographical distribution of the 221 known troglobiotic/troglomorphic species allowed us to select 19 karst areas that need conservation actions. Seven areas were considered to have urgent priority for conservation actions. The two richest areas correspond to the "iron quadrangle" with iron ore caves (67 spp.) and the "Açungui limestone group" (56 spp.). Both areas have several caves and are important aquifers. The use of the CCPi can prevent future losses because it helps assessors to select caves with priorities for conservation which should receive emergency attention in relation to protection, management and conservation actions.

  10. Karst and Caves of the Black Hills, South Dakota, USA; Karst y cuevas de las Black Hills, Dakota del Sur, EE.UU

    Energy Technology Data Exchange (ETDEWEB)

    Palmer, A. N.

    2016-07-01

    The caves of the Black Hills are located in Carboniferous limestone and dolomite of the Madison Formation in western South Dakota. The climate is semi-arid, and surface karst features are few. Entrances to known caves are rather small, and the two largest caves, Wind Cave and Jewel Cave, were discovered only in the late 1880s and in 1900, respectively. Intermittent exploration and mapping have been conducted by local volunteers, National Park Service staff, and the National Speleological Society. Jewel Cave, in Jewel Cave National Monument, contains 290 km of mapped passages; and Wind Cave, in Wind Cave National Park, contains 230 km. They are the third and sixth longest known caves in the world. (Author)

  11. CONTROL DEMONSTRATION OF THE RICEFIELD BREEDING MOSQUITO ANOPHELES ACONITUS DONITZ IN CENTRAL JAVA, USING; POECILIA RETICULATA THROUGH COMMUNITY PARTICIPATION : 1. EXPERIMENTAL DESIGN AND CONCEPT

    Directory of Open Access Journals (Sweden)

    Sustriayu Nalim

    2012-09-01

    Full Text Available Penelitian dilakukan untuk menjajagi cara memberantas vektor malaria Anopheles aconitus di sawah dengan ikan pemakan jentik Poecilia reticulata. Dalam penelitian ini terlihat bahwa dalam pelaksanaan program pemberantasan ini, partisipasi petani untuk mina padi dapat digalakkan melalui rapat kelompok tani. Program mina padi menye­diakan tempat hidup bagi ikan pemakan jentik. Rencana pelaksanaan program ini serta cara penggalakan   petani dibicarakan dalam makalah ini.

  12. Exposure of workers to radon progeny in the Baradla cave at Aggtelek

    International Nuclear Information System (INIS)

    Dezsoe, Z.

    2004-01-01

    By measuring the radon concentration using active technique in all parts of the cave visited in a tour and furthermore by assessing also the time spent at these locations, it is possible to determine the radon level averaged over a tour. The time averaged radon concentration along the route of the cave investigated has been estimated to 970 Bq/m 3 in summer and 410 Bq/m 3 in winter. We concluded that in 62 % of the year cave climate shows warm season and only in 38 % cold season Rn-levels. On this basis the yearly mean radon concentration averaged over the tour comes to 750 Bq/m3. This figure, however, should not be used to estimate exposure because the number of visitors is substantially higher in summer than in winter, so that the tour guides work much more in summer (about 750 tours) than in winter (about 270 tours). Taking this into account, the yearly mean Rn concentration is to 820 Bq/m 3 . Since the dose is delivered by radon progeny and not radon itself, one has to also assess the equilibrium factor representative in the cave. The data available in the literature show that F is always larger than under normal ambient conditions. Published values are between 0.5 - 0.8. As part of this work, several radon progeny concentration measurements have also been made in the Baradla cave at different locations. The results confirm the occurrence of high F-values in caves. Furthermore, the equilibrium factor is increased by the presence of humans (visitors or cavers). In the Virgin Cave, F = 0.5 - 0.6, which increases steadily to about 0.8 due to the activity of man. In this work, F = 0.8 has been used for the dose assessment. Using the ICRP 65 dosimetry model, exposure was calculated to be 4.04 mJhm 3 , which corresponds to an effective dose of 5.8 mSv. (P.A.)

  13. Occupational exposure to radon in Australian Tourist Caves an Australian-wide study of radon levels

    Energy Technology Data Exchange (ETDEWEB)

    Solomon, S B; Langroo, R; Peggie, J R [Australian Radiation Laboratory. Yallambie, VIC (Australia); Lyons, R G [University of Auckland, Auckland, (New Zealand). Department of Physics; James, J M [University of Sydney, Sydney, NSW (Australia). Department of Chemisty

    1996-02-01

    The study described in this report sets out to determine which Australian show caves have long- term radon levels in excess of the proposed action level of 1000 Bq m{sup -3}. The collaborative study between the Australian Radiation Laboratory (ARL), the University of Sydney and the University of Auckland, was carried out with the support of a Research Grant from Worksafe Australia. The aims of this study were to measure radon levels for each season over a period of one year, at representative sites in all developed show caves around Australia, to determine yearly average radon levels for each cave tour, based on these site measurements, to estimate the radiation doses to the tour guides employed in these caves, and to identify caves with radon concentrations in excess of the action level. (authors) 7 refs., 10 tabs., 2 figs.

  14. Concentration and stable carbon isotopic composition of CO2 in cave air of Postojnska jama, Slovenia

    Directory of Open Access Journals (Sweden)

    Magda Mandic

    2013-09-01

    Full Text Available Partial pressure of CO2 (pCO2 and its isotopic composition (δ13CairCO2 were measured in Postojnska jama, Slovenia, at 10 locations inside the cave and outside the cave during a one-year period. At all interior locations the pCO2 was higher and δ13CairCO2 lower than in the outside atmosphere. Strong seasonal fluctuations in both parameters were observed at locations deeper in the cave, which are isolated from the cave air circulation. By using a binary mixing model of two sources of CO2, one of them being the atmospheric CO2, we show that the excess of CO2 in the cave air has a δ13C value of -23.3 ± 0.7 ‰, in reasonable agreement with the previously measured soil-CO2 δ13C values. The stable isotope data suggest that soil CO2 is brought to the cave by drip water.

  15. Invertebrate fossils from cave sediments: A new proxy for pre-Quaternary paleoenvironments

    Czech Academy of Sciences Publication Activity Database

    Moldovan, O.T.; Mihevc, A.; Miko, L.; Constantin, S.; Meleg, I. N.; Petculescu, A.; Bosák, Pavel

    2011-01-01

    Roč. 8, č. 7 (2011), s. 1825-1837 ISSN 1726-4170 R&D Projects: GA AV ČR IAA300130701 Institutional research plan: CEZ:AV0Z30130516 Keywords : fossil invertebrates * Oribatida * Pliocene/Pleistocene * caves * caves (Slovenia) Subject RIV: DB - Geology ; Mineralogy Impact factor: 3.859, year: 2011

  16. Annual and transient signatures of gas exchange and transport in the Castañar de Ibor cave (Spain

    Directory of Open Access Journals (Sweden)

    Fernandez-Cortes A.

    2009-07-01

    Full Text Available The large microclimatic stability is a basic characteristic of the subterranean karst systems and causes a high sensitivity to changesin environmental conditions. High-accuracy monitoring of Castañar de Ibor cave (Spain determined the temporal evolution of theaerodynamic processes and ventilation rate by tracking CO2 and 222Rn levels over a twelve-month period. This cave is characterizedby a very stable microclimate, with high and relatively constant radon content (the mean value is 32200 Bq/m3, roughly, and thestandard deviation is 7600 Bq/m3 and a moderate and quite stable CO2 concentration (the mean value is 3730 ppm and the standarddeviation is 250 ppm. Beside the general patterns of cave microclimate throughout an annual cycle, some particular microclimaticprocesses are described with regard to the gas exchange between the cave and the outside atmosphere. There is a complexmicroclimatic functional relationship between the meteorological and cave microclimate conditions and the diffusion and flow of tracergases from the fractures and the pore system of soil and host rock to cave atmosphere. Transient variations of tracer gas on cave airare controlled by natural barometric fluxes and anthropogenic forced ventilation due to uncontrolled opening of cave entrance. Theshort-term fluctuations of gas levels on cave air reveal distinct patterns during the exhalation process of theses gases from the netof fissures and pores to the cave atmosphere, depending on the isolation effect of soil and host rock.

  17. The Unicorn Cave, Southern Harz Mountains, Germany: From known passages to unknown extensions with the help of geophysical surveys

    Science.gov (United States)

    Kaufmann, Georg; Nielbock, Ralf; Romanov, Douchko

    2015-12-01

    In soluble rocks (limestone, dolomite, anhydrite, gypsum, …), fissures and bedding partings can be enlarged with time by both physical and chemical dissolution of the host rock. With time, larger cavities evolve, and a network of cave passages can evolve. If the enlarged cave voids are not too deep under the surface, geophysical measurements can be used to detect, identify and trace these karst structures, e.g.: (i) gravity revealing air- and sediment-filled cave voids through negative Bouguer anomalies, (ii) electrical resistivity imaging (ERI) mapping different infillings of cavities either as high resistivities from air-filled voids or dry soft sediments, or low resistivities from saturated sediments, and (iii) groundwater flow through electrical potential differences (SP) arising from dislocated ionic charges from the walls of the underground flow paths. We have used gravity, ERI, and SP methods both in and above the Unicorn Cave located in the southern Harz Mountains in Germany. The Unicorn Cave is a show cave developed in the Werra dolomite formation of the Permian Zechstein sequence, characterised by large trunk passages interrupted by larger rooms. The overburden of the cave is only around 15 m, and passages are filled with sediments reaching infill thicknesses up to 40 m. We present results from our geophysical surveys above the known cave and its northern and southern extension, and from the cave interior. We identify the cave geometry and its infill from gravity and ERI measurements, predict previously unknown parts of the cave, and subsequently confirm the existence of these new passages through drilling. From the wealth of geophysical data acquired we derive a three-dimensional structural model of the Unicorn Cave and its surrounding, especially the cave infill.

  18. Evolution of cave Axiokebuita and Speleobregma (Scalibregmatidae, Annelida)

    DEFF Research Database (Denmark)

    Martinez Garcia, Alejandro; Di Domenico, Maikon; Worsaae, Katrine

    2013-01-01

    The evolutionary history of Axiokebuita and Speleobregma, two poorly known lineages of annelids exclusive from deep-sea or marine caves but always from crevicular habitats, is explored here. Speleobregma lanzaroteum Bertelsen, 1986, and Axiokebuita cavernicola sp. n. are described from anchialine...... ciliated palps. Our results support two independent cave colonization events, favoured by the preadaptation of the members of Axiokebuita-Speleobregma lineage to crevicular habitats.......The evolutionary history of Axiokebuita and Speleobregma, two poorly known lineages of annelids exclusive from deep-sea or marine caves but always from crevicular habitats, is explored here. Speleobregma lanzaroteum Bertelsen, 1986, and Axiokebuita cavernicola sp. n. are described from anchialine...

  19. Reconstruction of bomb 14C time history recorded in the stalagmite from Postojna Cave

    International Nuclear Information System (INIS)

    Vokal, B.; Genty, D.; Obelic, B.

    2002-01-01

    The karstic caves provide valuable resources for reconstruction of environmental conditions on the continent in the past. This is possible due to the great stability of climatic conditions within a cave. Secondary minerals deposited in caves, known as speleothems, preserve records of long-term climatic and environmental changes at the site of their deposition and in the vicinity. The purity of speleothems and their chemical and physical stability make them exceptionally well suited for detailed geochemical and isotopic analysis

  20. Environmental record in detrital cave sediments in the Botovskaya and Dolganskaya Jama caves (Russian Federation)

    Czech Academy of Sciences Publication Activity Database

    Lisá, Lenka; Kadlec, Jaroslav; Chadima, Martin; Hercman, H.; Oberhansli, H.; Osincev, A.

    2006-01-01

    Roč. 8, - (2006), A10273-A10273 ISSN 1029-7006. [European Geosciences Union General Assembly. 02.04.2006-07.04.2006, Vienna] Institutional research plan: CEZ:AV0Z30130516 Keywords : cave * sediments * micromorphology * magnetic properties * provenance Subject RIV: DB - Geology ; Mineralogy http://www.cosis.net/abstracts/EGU06/10273/EGU06-J-10273-2.pdf

  1. Mamalia, Chiroptera, Vespertilionidae: Filling hibernacula distribution gaps for cave roosting bats from Iowa (U.S.A..

    Directory of Open Access Journals (Sweden)

    Dixon, J. W.

    2010-01-01

    Full Text Available Adequate roost sites for hibernacula are an important factor in the distribution and abundance of temperate batspecies and knowledge of specific hibernacula is necessary to make sound management decisions. Caves are recognized asone of the most important roosting sites for bats, yet surveys in caves are uncommon in North America. This paper presentsdata on the distribution and abundance of bats hibernating in Iowa (U.S.A. caves and includes new hibernacula records.These are the first published records of bats in Iowa caves in almost 25 years.

  2. Basaltic caves at Craters of the Moon National Monument and Preserve as analogs for Mars

    Science.gov (United States)

    Hinman, N. W.; Richardson, C. D.; McHenry, L.; Scott, J. R.

    2010-12-01

    Basaltic caves and lava tubes offer stable physicochemical conditions for formation of secondary minerals. Such features, putatively observed on Mars, intercept groundwater to weather country rock, leading to formation of secondary minerals. Further, caves are stable environments to search for evidence of past life, as they could offer protection from the oxidizing martian atmosphere. Searching for signs of life in a cave that could protect bio/organic compounds would preclude the need for risky drilling on Mars. Craters of the Moon National Monument (COM) offers an opportunity to study caves in Holocene iron-rich basalt flows to characterize secondary mineral deposits and search for organic compounds associated with secondary minerals; COM basalts are a good analog for martian basalts because of their high iron but other elements are higher at COM than on Mars. The Blue Dragon flow (~2.1 ka) contains the majority of the accessible caves and lava tubes. Two types of secondary mineral deposits were observed in these caves: ceiling coatings and crack or floor precipitates. Hematite, silica, and calcite comprise ceiling coatings. The crack and floor precipitates are white, efflorescent deposits in cavities along cave walls and ceilings or in localized mounds on cave floors. The secondary minerals in crack and floor precipitates are mainly thenardite and mirabilite with some minor concentrations of trona and/or burkeite. Organic compounds were found associated with the efflorescent deposits. Formation of the deposits is likely due to chemical leaching of basalt by meteoritic water. To test this, fluids collected from the ceiling and walls of the caves were analyzed. Solutions were modeled with the geochemical code, PHREEQC. The model tracked composition as water evaporated. Selected minerals were allowed to precipitate as they became oversaturated. Among the first minerals to become oversaturated were quartz and calcite, which are observed in ceiling deposits. Iron

  3. Direct measurement of present-day tectonic movement and associated radon flux in Postojna Cave, Slovenia

    Czech Academy of Sciences Publication Activity Database

    Šebela, S.; Vaupotič, J.; Košťák, Blahoslav; Stemberk, Josef

    2010-01-01

    Roč. 72, č. 1 (2010), s. 21-34 ISSN 1090-6924 R&D Projects: GA ČR GA205/09/2024; GA MŠk MEB091005 Institutional research plan: CEZ:AV0Z30460519 Keywords : fault displacements * radon flux * Postojna Cave Subject RIV: DB - Geology ; Mineralogy Impact factor: 0.842, year: 2010 www.caves.org/pub/journal/Journal_of_Cave_and_Karst_Studies_volume_72.htm

  4. THE STRUCTURE OF SOIL MESOFAUNA AND MACROFAUNA IN GRODA CAVE, GUNUNGKIDUL

    Directory of Open Access Journals (Sweden)

    Andri Prasetyo

    2016-10-01

      The number of soil macrofauna and mesofauna in Groda Cave can be used as an indicator of bats abundance since bat droppings are food for macrofauna and mesofauna soils. The purpose of this study are to determine the community structure of Groda Cave soil macrofauna and mesofauna  and its correlation with the content of N, P, K. The method used is the observation, taking of macrofauna and mesofauna soils using pit fall traps and tool green technique. Testing the N, P, K of the soil was conducted in Balai Pengkajian Teknologi Pertanian (BPTP Yogyakarta. The analysis technique used Shannon diversity index-Wienner, Margaleff wealth index, frequency of attendance of  macrofauna and mesofauna soils and its correlation with N, P, K of the land in Groda Cave. The results obtained are that six families mesofauna and 20 families macrofauna soils with moderate diversity index, the highest wealth index was in the Margaleff lit zone, the frequency of the highest attendance in the light zone is Formicidae (0.12, ie dim zones Isotomidae and Diptera (a brown (0.04 and the dark zone is Gryllacididae (0.05. The correlation showed the more the content of N, P, K, the fewer mesofauna and macrofauna soils were found. The uniqueness found in this study is the discovery of one species of Isotomidae in Groda Cave, which has been considered spreading only in Sulawesi, Sumatera, Bali, Lombok, Ternate and Papua.   Keywords: community structure, mesofauna soil, macrofauna soil, Groda Cave

  5. Radon Concentration in Caves of Croatia - Assesing Effective Radon Doses for Occupational Workers and Visitors

    International Nuclear Information System (INIS)

    Radolic, V.; Miklavcic, I.; Poje, M.; Stanic, D.; Vukovic, B.; Paar, D.

    2011-01-01

    Radon monitoring at potentially highly radioactive location such as caves is important to assess the radiological hazards to occupational workers and occasional visitors. In its Publication 65 the ICRP has produced recommendations dealing with exposure to elevated background radiation, in particular, the risk associated with the inhalation of radon and radon progeny. Recommended annual effective dose from radon 222Rn and its short-lived progeny for workers should not exceed 20 mSv and for occasional users (visitors) the same recommendation is 1 mSv. Measurements were performed with series of track etched detectors (LR115 - type II) in several caves in Croatia. The obtained values for the radon concentration ranged from ambient values up to several thousand Bq m -3 . Radon concentration was measured in about 20 caves of Velebit and Zumberak mountains and the highest radon concentration was in Lubuska jama (3.8 kBq m -3 ) and cave Dolaca (21.8 kBq m -3 ), respectively. Djurovica cave is especially interesting because of its huge tourist potential due to its location bellow Dubrovnik airport. Its mean annual radon concentration of 17.6 kBq m -3 classifies Djurovica cave among caves with high radon concentration. A visitor during half an hour visit at summer time would receive an effective dose of 30.6 μSv. Calculated mean dose rate of 44 μSv/h means that workers (mainly tourist guides) should limit their time inside cave to 454 hours per year. Manita pec is the only cave open for tourists on the territory of Paklenica National Park. The preliminary radon measurements performed during summer 2010, gave an average radon concentration of 1.1 kBq m -3 . An exposure to average dose rate of 3.7 μSv/h means that the tourist guides would receive an effective dose of 0.42 mSv during summer period according to their working schedule. A visitor during half an hour visits would receive an effective dose of 1.86 μSv. (author)

  6. Cell phone-generated radio frequency electromagnetic field effects on the locomotor behaviors of the fishes Poecilia reticulata and Danio rerio.

    Science.gov (United States)

    Lee, David; Lee, Joshua; Lee, Imshik

    2015-01-01

    The locomotor behavior of small fish was characterized under a cell phone-generated radio frequency electromagnetic field (RF EMF). The trajectory of movement of 10 pairs of guppy (Poecilia reticulate) and 15 pairs of Zebrafish (Danio rerio) in a fish tank was recorded and tracked under the presence of a cell phone-generated RF EMF. The measures were based on spatial and temporal distributions. A time-series trajectory was utilized to emphasize the dynamic nature of locomotor behavior. Fish movement was recorded in real-time. Their spatial, velocity, turning angle and sinuosity distribution were analyzed in terms of F(v,x), P[n(x,t)], P(v), F (θ) and F(s), respectively. In addition, potential temperature elevation caused by a cellular phone was also examined. We demonstrated that a cellular phone-induced temperature elevation was not relevant, and that our measurements reflected RF EMF-induced effects on the locomotor behavior of Poecilia reticulata and Danio rerio. Fish locomotion was observed under normal conditions, in the visual presence of a cell phone, after feeding, and under starvation. Fish locomotor behavior was random both in normal conditions and in the presence of an off-signaled cell phone. However, there were significant changes in the locomotion of the fish after feeding under the RF EMF. The locomotion of the fed fish was affected in terms of changes in population and velocity distributions under the presence of the RF EMF emitted by the cell phone. There was, however, no significant difference in angular distribution.

  7. Stability numerical analysis of soil cave in karst area to drawdown of underground water level

    Science.gov (United States)

    Mo, Yizheng; Xiao, Rencheng; Deng, Zongwei

    2018-05-01

    With the underground water level falling, the reliable estimates of the stability and deformation characteristics of soil caves in karst region area are required for analysis used for engineering design. Aimed at this goal, combined with practical engineering and field geotechnical test, detail analysis on vertical maximum displacement of top, vertical maximum displacement of surface, maximum principal stress and maximum shear stress were conducted by finite element software, with an emphasis on two varying factors: the size and the depth of soil cave. The calculations on the soil cave show that, its stability of soil cave is affected by both the size and depth, and only when extending a certain limit, the collapse occurred along with the falling of underground water; Additionally, its maximum shear stress is in arch toes, and its deformation curve trend of maximum displacement is similar to the maximum shear stress, which further verified that the collapse of soil cave was mainly due to shear-failure.

  8. Relation of desert pupfish abundance to selected environmental variables in natural and manmade habitats in the Salton Sea basin

    Science.gov (United States)

    Martin, B.A.; Saiki, M.K.

    2005-01-01

    We assessed the relation between abundance of desert pupfish, Cyprinodon macularius, and selected biological and physicochemical variables in natural and manmade habitats within the Salton Sea Basin. Field sampling in a natural tributary, Salt Creek, and three agricultural drains captured eight species including pupfish (1.1% of the total catch), the only native species encountered. According to Bray-Curtis resemblance functions, fish species assemblages differed mostly between Salt Creek and the drains (i.e., the three drains had relatively similar species assemblages). Pupfish numbers and environmental variables varied among sites and sample periods. Canonical correlation showed that pupfish abundance was positively correlated with abundance of western mosquitofish, Gambusia affinis, and negatively correlated with abundance of porthole livebearers, Poeciliopsis gracilis, tilapias (Sarotherodon mossambica and Tilapia zillii), longjaw mudsuckers, Gillichthys mirabilis, and mollies (Poecilia latipinnaandPoecilia mexicana). In addition, pupfish abundance was positively correlated with cover, pH, and salinity, and negatively correlated with sediment factor (a measure of sediment grain size) and dissolved oxygen. Pupfish abundance was generally highest in habitats where water quality extremes (especially high pH and salinity, and low dissolved oxygen) seemingly limited the occurrence of nonnative fishes. This study also documented evidence of predation by mudsuckers on pupfish. These findings support the contention of many resource managers that pupfish populations are adversely influenced by ecological interactions with nonnative fishes. ?? Springer 2005.

  9. Steppe lion remains imported by Ice Age spotted hyenas into the Late Pleistocene Perick Caves hyena den in northern Germany

    Science.gov (United States)

    Diedrich, Cajus G.

    2009-05-01

    Upper Pleistocene remains of the Ice Age steppe lion Panthera leo spelaea (Goldfuss, 1810) have been found in the Perick Caves, Sauerland Karst, NW Germany. Bones from many hyenas and their imported prey dating from the Lower to Middle Weichselian have also been recovered from the Perick Cave hyena den. These are commonly cracked or exhibit deep chew marks. The absence of lion cub bones, in contrast to hyena and cave bear cub remains in the Perick Caves, and other caves of northern Germany, excludes the possibility that P. leo spelaea used the cave for raising cubs. Only in the Wilhelms Cave was a single skeleton of a cub found in a hyena den. Evidence of the chewing, nibbling and cracking of lion bones and crania must have resulted from the importation and destruction of lion carcasses (4% of the prey fauna). Similar evidence was preserved at other hyena den caves and open air sites in Germany. The bone material from the Perick and other Central European caves points to antagonistic hyena and lion conflicts, similar to clashes of their modern African relatives.

  10. Predicting the Occurrence of Cave-Inhabiting Fauna Based on Features of the Earth Surface Environment.

    Science.gov (United States)

    Christman, Mary C; Doctor, Daniel H; Niemiller, Matthew L; Weary, David J; Young, John A; Zigler, Kirk S; Culver, David C

    2016-01-01

    One of the most challenging fauna to study in situ is the obligate cave fauna because of the difficulty of sampling. Cave-limited species display patchy and restricted distributions, but it is often unclear whether the observed distribution is a sampling artifact or a true restriction in range. Further, the drivers of the distribution could be local environmental conditions, such as cave humidity, or they could be associated with surface features that are surrogates for cave conditions. If surface features can be used to predict the distribution of important cave taxa, then conservation management is more easily obtained. We examined the hypothesis that the presence of major faunal groups of cave obligate species could be predicted based on features of the earth surface. Georeferenced records of cave obligate amphipods, crayfish, fish, isopods, beetles, millipedes, pseudoscorpions, spiders, and springtails within the area of Appalachian Landscape Conservation Cooperative in the eastern United States (Illinois to Virginia and New York to Alabama) were assigned to 20 x 20 km grid cells. Habitat suitability for these faunal groups was modeled using logistic regression with twenty predictor variables within each grid cell, such as percent karst, soil features, temperature, precipitation, and elevation. Models successfully predicted the presence of a group greater than 65% of the time (mean = 88%) for the presence of single grid cell endemics, and for all faunal groups except pseudoscorpions. The most common predictor variables were latitude, percent karst, and the standard deviation of the Topographic Position Index (TPI), a measure of landscape rugosity within each grid cell. The overall success of these models points to a number of important connections between the surface and cave environments, and some of these, especially soil features and topographic variability, suggest new research directions. These models should prove to be useful tools in predicting the

  11. Past surface conditions and speleogenesis as inferred from cave sediments in the Great Cave of Șălitrari Mountain (SW Romania

    Directory of Open Access Journals (Sweden)

    Cristina M. Pușcaș

    2010-08-01

    Full Text Available Abstract In one of the passages in the Great Cave of Șălitrari Mountain the floor is completely covered by an alluvial deposit at least 6 m in thickness, ranging from boulders, and cobbles, to sand and clay, topped by a layer of dry bat guano. Sediment and mineral samples collected from six profiles underwent broad analyses to determine their petrological and mineralogical makeup, grain-size distribution, and paleoclimatic significance. The complicated facies alternation suggests frequent changes in the former stream’s hydrological parameters, with frequent flooding, leading to the hypothesis that the climate was somewhat wetter than today. Both the mineralogical composition of the sediment (ranging from quartz, mica, gypsum, phosphates, and calcite to garnet, zircon, titanite, olivine, serpentine, tourmaline, sphalerite, pyrite/chalcopyrite, and feldspars and the petrological composition of the larger clasts (limestone, sandstone, mudstone, granitoids, serpentinite, amphibolite, diorite, gneiss, quartzite, microconglomerate, and schist ascribe the potential source rocks to an area with contrasting lithologies, such as amphibolites, felsic and basic metaigneous, and metasedimentary rocks, mixed with a variety of detritic rocks. These rock types are not entirely comprised by the catchment area of the modern Presacina Brook, thus implying that due either to hydrological conditions, or to changes in the base level caused by river down cutting or active tectonics, the former source area was much more extensive. Based on morphological and sedimentological criteria, the cave started under pipe-full flow conditions, and further evolved during a prolonged and complex vadose phase. Evidence to support the existence of hypogene conditions is also present. Once the underground stream left the cave and most of the sediment was removed, speleothem precipitation was initiated. In this contribution we put forward evidence that argue for an extra

  12. Using the CAVE virtual-reality environment as an aid to 3-D electromagnetic field computation

    International Nuclear Information System (INIS)

    Turner, L.R.; Levine, D.; Huang, M.; Papka, M.

    1995-01-01

    One of the major problems in three-dimensional (3-D) field computation is visualizing the resulting 3-D field distributions. A virtual-reality environment, such as the CAVE, (CAVE Automatic Virtual Environment) is helping to overcome this problem, thus making the results of computation more usable for designers and users of magnets and other electromagnetic devices. As a demonstration of the capabilities of the CAVE, the elliptical multipole wiggler (EMW), an insertion device being designed for the Advanced Photon Source (APS) now being commissioned at Argonne National Laboratory (ANL), wa made visible, along with its fields and beam orbits. Other uses of the CAVE in preprocessing and postprocessing computation for electromagnetic applications are also discussed

  13. Blow Hole Cave: An unroofed cave on San Salvador Island, the Bahamas, and its importance for detection of paleokarst caves on fossil carbonate platforms

    Czech Academy of Sciences Publication Activity Database

    Bosák, Pavel; Mylroie, J. E.; Hladil, Jindřich; Carew, J. L.; Slavík, Ladislav

    2002-01-01

    Roč. 31, č. 3 (2002), s. 51-74 ISSN 0583-6050. [Karstological School - Classical Karst: Types of Karst /10./. Pstojna, 26.06.2002-28.06.2002] R&D Projects: GA AV ČR IAA3013809; GA AV ČR IAA3013209 Keywords : carbonate platforms * unroofed caves * gamma-ray spectrometry and wellogging Subject RIV: DB - Geology ; Mineralogy http://carsologica.zrc-sazu.si/downloads/313/slavik.pdf

  14. Detection of "bath salts" and other novel psychoactive substances in hair samples of ecstasy/MDMA/"Molly" users.

    Science.gov (United States)

    Palamar, Joseph J; Salomone, Alberto; Vincenti, Marco; Cleland, Charles M

    2016-04-01

    Ecstasy (MDMA) in the US is commonly adulterated with other drugs, but research has not focused on purity of ecstasy since the phenomenon of "Molly" (ecstasy marketed as pure MDMA) arose in the US. We piloted a rapid electronic survey in 2015 to assess use of novel psychoactive substances (NPS) and other drugs among 679 nightclub/festival-attending young adults (age 18-25) in New York City. A quarter (26.1%) of the sample provided a hair sample to be analyzed for the presence of select synthetic cathinones ("bath salts") and some other NPS. Samples were analyzed using fully validated UHPLC-MS/MS methods. To examine consistency of self-report, analyses focused on the 48 participants with an analyzable hair sample who reported lifetime ecstasy/MDMA/Molly use. Half (50.0%) of the hair samples contained MDMA, 47.9% contained butylone, and 10.4% contained methylone. Of those who reported no lifetime use of "bath salts", stimulant NPS, or unknown pills or powders, about four out of ten (41.2%) tested positive for butylone, methylone, alpha-PVP, 5/6-APB, or 4-FA. Racial minorities were more likely to test positive for butylone or test positive for NPS after reporting no lifetime use. Frequent nightclub/festival attendance was the strongest predictor of testing positive for MDMA, butylone, or methylone. Results suggest that many ecstasy-using nightclub/festival attendees may be unintentionally using "bath salts" or other NPS. Prevention and harm reduction education is needed for this population and "drug checking" (e.g., pill testing) may be beneficial for those rejecting abstinence. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  15. Simulated effects of increased groundwater withdrawals in the Cave Springs area, Hixson, Tennessee

    Science.gov (United States)

    Haugh, Connor J.

    2014-01-01

    Concern for future water supplies in Tennessee has grown in recent years as a result of increased awareness of competing needs, the impact of droughts, and the need for more water to support growing populations. The U.S. Geological Survey conducts investigations to improve the knowledge about interactions of geology, climate, humans, and ecosystems with the water cycle, which is critical to understanding and optimizing water availability. The Hixson Utility District in Hamilton County, Tennessee, uses groundwater resources in the Cave Springs area as a water supply, withdrawing water from two well fields located at Cave Springs and Walkers Corner. Historically, Hixson Utility District has withdrawn about 5 million gallons per day (Mgal/d) at the Cave Springs well field and between 2 and 3 Mgal/d at the Walkers Corner well field. To assess the capacity of the groundwater resources in the Cave Springs area to meet future demands, four different scenarios of increased groundwater withdrawals were analyzed using computer model simulations.

  16. Cooperative hunting and meat sharing 400-200 kya at Qesem Cave, Israel.

    Science.gov (United States)

    Stiner, Mary C; Barkai, Ran; Gopher, Avi

    2009-08-11

    Zooarchaeological research at Qesem Cave, Israel demonstrates that large-game hunting was a regular practice by the late Lower Paleolithic period. The 400- to 200,000-year-old fallow deer assemblages from this cave provide early examples of prime-age-focused ungulate hunting, a human predator-prey relationship that has persisted into recent times. The meat diet at Qesem centered on large game and was supplemented with tortoises. These hominins hunted cooperatively, and consumption of the highest quality parts of large prey was delayed until the food could be moved to the cave and processed with the aid of blade cutting tools and fire. Delayed consumption of high-quality body parts implies that the meat was shared with other members of the group. The types of cut marks on upper limb bones indicate simple flesh removal activities only. The Qesem cut marks are both more abundant and more randomly oriented than those observed in Middle and Upper Paleolithic cases in the Levant, suggesting that more (skilled and unskilled) individuals were directly involved in cutting meat from the bones at Qesem Cave. Among recent humans, butchering of large animals normally involves a chain of focused tasks performed by one or just a few persons, and butchering guides many of the formalities of meat distribution and sharing that follow. The results from Qesem Cave raise new hypotheses about possible differences in the mechanics of meat sharing between the late Lower Paleolithic and Middle Paleolithic.

  17. Repeated and time-correlated morphological convergence in cave-dwelling harvestmen (Opiliones, Laniatores from Montane Western North America.

    Directory of Open Access Journals (Sweden)

    Shahan Derkarabetian

    Full Text Available BACKGROUND: Many cave-dwelling animal species display similar morphologies (troglomorphism that have evolved convergent within and among lineages under the similar selective pressures imposed by cave habitats. Here we study such ecomorphological evolution in cave-dwelling Sclerobuninae harvestmen (Opiliones from the western United States, providing general insights into morphological homoplasy, rates of morphological change, and the temporal context of cave evolution. METHODOLOGY/PRINCIPAL FINDINGS: We gathered DNA sequence data from three independent gene regions, and combined these data with Bayesian hypothesis testing, morphometrics analysis, study of penis morphology, and relaxed molecular clock analyses. Using multivariate morphometric analysis, we find that phylogenetically unrelated taxa have convergently evolved troglomorphism; alternative phylogenetic hypotheses involving less morphological convergence are not supported by Bayesian hypothesis testing. In one instance, this morphology is found in specimens from a high-elevation stony debris habitat, suggesting that troglomorphism can evolve in non-cave habitats. We discovered a strong positive relationship between troglomorphy index and relative divergence time, making it possible to predict taxon age from morphology. Most of our time estimates for the origin of highly-troglomorphic cave forms predate the Pleistocene. CONCLUSIONS/SIGNIFICANCE: While several regions in the eastern and central United States are well-known hotspots for cave evolution, few modern phylogenetic studies have addressed the evolution of cave-obligate species in the western United States. Our integrative studies reveal the recurrent evolution of troglomorphism in a perhaps unexpected geographic region, at surprisingly deep time depths, and in sometimes surprising habitats. Because some newly discovered troglomorphic populations represent undescribed species, our findings stress the need for further biological

  18. Indoor radon concentration levels in Mexican caves, using nuclear track methodology, and the relationship with living habits of the bats

    International Nuclear Information System (INIS)

    Espinosa, G.; Golzarri, J.I.; Vega-Orihuela, E.; Morales-Malacara, J.B.

    2013-01-01

    This work presents the results of a study of the radon levels in four caves in Mexico: Los Riscos Cave and El Judio Cave in the State of Queretaro, and Coyozochico Cave and Karmidas Cave in the State of Puebla. The measurements were made using the passive closed-end cup system, with CR-39 (Lantrack R ) as detection material, and following protocols established for the measurement of indoor radon, developed at the Dosimetry Applications Project of the Physics Institute of the Universidad Nacional Autonoma de Mexico. The radon concentration at one location with Karmidas Cave reached more than 60,000 Bq/m 3 , while concentrations in the other three caves varied from 83.1-1216.0 Bq/m 3 , was found. During the study was observed an interesting coincidence between the radon concentration distribution inside the caves, and the bat colonies location. In general, the bat colonies are located at the medium or low radon concentration levels zones. (author)

  19. 3D numerical modeling of longwall mining with top-coal caving

    Energy Technology Data Exchange (ETDEWEB)

    Yasitli, N.E.; Unver, B. [Hacettepe University, Ankara (Turkey). Dept. of Mining Engineering

    2005-02-01

    Top-coal caving is the key factor affecting the efficiency of production at thick-coal seams. During production of top coal by caving behind the face not only a significant amount of coal is lost in the goaf but the coal drawn by means of caving is diluted considerably with surrounding rock. Therefore, it is not possible to carry out an efficient production operation unless caving of top coal behind the face is optimized. In this paper, results of 3D modeling of the top-coal-caving mechanism by using the finite difference code FLAC3{sup D} at the M3 longwall panel of the Omerler Underground Mine located at Tuncbilek (Turkey) are presented. According to the modeling results, maximum vertical abutment stresses were formed at a distance of 7m in front of the face. An analysis of the conditions of top coal has revealed that a 1.5 m thick layer of coal just above the shield supports is well fractured. However, a 3.5 m thick layer of coal above the fractured part is either not fractured or is fractured in the form of large blocks leading to obstruction of windows of shields during coal drawing. It is concluded that, in order to decrease dilution and increase extraction ratio and efficiency of operation, top coal should be as uniformly fractured as possible. Hence, an efficient and continuous coal flowing behind the face can be maintained. A special pre-fracture blasting strategy just sufficient enough to form cracks in the top coal is suggested by means of comparing with the results of numerical modeling.

  20. Seasonal variation measurements of radon levels in caves using SSNTD method

    International Nuclear Information System (INIS)

    Espinosa, G.; Golzarri, J.I.; Gammage, R.B.; Sajo-Bohus, L.; Viccon-Pale, J.; Signoret-Poillon, M.

    2008-01-01

    The results of radon concentration measurements inside of the Gabriel caves of Mexico, during three consecutive two-month periods covering almost three seasons, are reported in the present work. The radio-ecological importance of this site is related to the radon and its concentration-dynamic behavior in the cave. Further interest in radiation safety motivated this initiative since routine biological field work is done, with people spending long periods of time there. CR-39 passive nuclear track detector was chosen for this survey. Radon concentration levels decrease during the rainy season and show different values depending on the ventilation and geometeorological structure. Measured values range between 956 and 4931Bqm -3 , an indication that radon doses may exceed the allowed values for workers. This project is part of a larger study of indoor radon alpha emitters in Mexican caves

  1. Seasonal variation measurements of radon levels in caves using SSNTD method

    Energy Technology Data Exchange (ETDEWEB)

    Espinosa, G. [Instituto de Fisica, Universidad Nacional Autonoma de Mexico, Apartado Postal 20-364, 01000 Mexico, D.F. (Mexico)], E-mail: espinosa@fisica.unam.mx; Golzarri, J.I. [Instituto de Fisica, Universidad Nacional Autonoma de Mexico, Apartado Postal 20-364, 01000 Mexico, D.F. (Mexico); Gammage, R.B. [Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831-6480 (United States); Sajo-Bohus, L. [Departamento de Fisica, Universidad Simon Bolivar (Venezuela); Viccon-Pale, J.; Signoret-Poillon, M. [El Hombre y su Ambiente, UAM-Xochimilco, Mexico D.F. (Mexico)

    2008-08-15

    The results of radon concentration measurements inside of the Gabriel caves of Mexico, during three consecutive two-month periods covering almost three seasons, are reported in the present work. The radio-ecological importance of this site is related to the radon and its concentration-dynamic behavior in the cave. Further interest in radiation safety motivated this initiative since routine biological field work is done, with people spending long periods of time there. CR-39 passive nuclear track detector was chosen for this survey. Radon concentration levels decrease during the rainy season and show different values depending on the ventilation and geometeorological structure. Measured values range between 956 and 4931Bqm{sup -3}, an indication that radon doses may exceed the allowed values for workers. This project is part of a larger study of indoor radon alpha emitters in Mexican caves.

  2. Digital Preservation of Ancient Maya Cave Architecture: Recent Field Efforts in Quintana Roo, Mexico

    Science.gov (United States)

    Rissolo, D.; Lo, E.; Hess, M. R.; Meyer, D. E.; Amador, F. E.

    2017-08-01

    The presence of ancient Maya shrines in caves serves as unequivocal evidence for the ritual appropriation of these subterranean spaces and their significance with respect to Maya religious practice. Detailed study of the miniature masonry temples and altar features in the caves of Quintana Roo, Mexico reveals a strong stylistic and likely functional correspondence between these structures and their terrestrial counterparts at Postclassic sites. The Proyecto Arquitectura Subterranea de Quintana Roo (coordinated by the Center of Interdisciplinary Science for Art, Architecture, and Archaeology, or CISA3, at the University of California, San Diego and in collaboration with the Instituto Nacional de Antropologia e Historia in Mexico) is conducting a survey and program of digital documentation of both the pristine and impacted cave shrines of the region. Once an area is developed and populated, and access is opened to caves containing ancient architectural features, they are soon vandalized - often resulting in the complete obliteration of these rare miniature buildings and their diagnostic architectural elements. This emergent situation necessitates the use of rapid reality-capture tools; however, the physical challenges of working in caves requires researchers of adapt increasingly common architectural documentation methodologies to more adverse field conditions.

  3. Passive seismic tomography application for cave monitoring in DOZ underground mine PT. Freeport Indonesia

    International Nuclear Information System (INIS)

    Nurhandoko, Bagus Endar B.; Wely, Woen; Setiadi, Herlan; Riyanto, Erwin

    2015-01-01

    It is already known that tomography has a great impact for analyzing and mapping unknown objects based on inversion, travel time as well as waveform inversion. Therefore, tomography has used in wide area, not only in medical but also in petroleum as well as mining. Recently, tomography method is being applied in several mining industries. A case study of tomography imaging has been carried out in DOZ ( Deep Ore Zone ) block caving mine, Tembagapura, Papua. Many researchers are undergoing to investigate the properties of DOZ cave not only outside but also inside which is unknown. Tomography takes a part for determining this objective.The sources are natural from the seismic events that caused by mining induced seismicity and rocks deformation activity, therefore it is called as passive seismic. These microseismic travel time data are processed by Simultaneous Iterative Reconstruction Technique (SIRT). The result of the inversion can be used for DOZ cave monitoring. These information must be used for identifying weak zone inside the cave. In addition, these results of tomography can be used to determine DOZ and cave information to support mine activity in PT. Freeport Indonesia

  4. Passive seismic tomography application for cave monitoring in DOZ underground mine PT. Freeport Indonesia

    Energy Technology Data Exchange (ETDEWEB)

    Nurhandoko, Bagus Endar B.; Wely, Woen; Setiadi, Herlan [WISFIR Laboratory, Earth Physics and Complex System Division, Physics Department, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung (Indonesia); Riyanto, Erwin [Geotechnical and Hydrology PT. Freeport Indonesia wonbin-ww@hotmail.com (Indonesia)

    2015-04-16

    It is already known that tomography has a great impact for analyzing and mapping unknown objects based on inversion, travel time as well as waveform inversion. Therefore, tomography has used in wide area, not only in medical but also in petroleum as well as mining. Recently, tomography method is being applied in several mining industries. A case study of tomography imaging has been carried out in DOZ ( Deep Ore Zone ) block caving mine, Tembagapura, Papua. Many researchers are undergoing to investigate the properties of DOZ cave not only outside but also inside which is unknown. Tomography takes a part for determining this objective.The sources are natural from the seismic events that caused by mining induced seismicity and rocks deformation activity, therefore it is called as passive seismic. These microseismic travel time data are processed by Simultaneous Iterative Reconstruction Technique (SIRT). The result of the inversion can be used for DOZ cave monitoring. These information must be used for identifying weak zone inside the cave. In addition, these results of tomography can be used to determine DOZ and cave information to support mine activity in PT. Freeport Indonesia.

  5. Evaluating visual discomfort in stereoscopic projection-based CAVE system with a close viewing distance

    Science.gov (United States)

    Song, Weitao; Weng, Dongdong; Feng, Dan; Li, Yuqian; Liu, Yue; Wang, Yongtian

    2015-05-01

    As one of popular immersive Virtual Reality (VR) systems, stereoscopic cave automatic virtual environment (CAVE) system is typically consisted of 4 to 6 3m-by-3m sides of a room made of rear-projected screens. While many endeavors have been made to reduce the size of the projection-based CAVE system, the issue of asthenopia caused by lengthy exposure to stereoscopic images in such CAVE with a close viewing distance was seldom tangled. In this paper, we propose a light-weighted approach which utilizes a convex eyepiece to reduce visual discomfort induced by stereoscopic vision. An empirical experiment was conducted to examine the feasibility of convex eyepiece in a large depth of field (DOF) at close viewing distance both objectively and subjectively. The result shows the positive effects of convex eyepiece on the relief of eyestrain.

  6. Hydrothermal phenomena in Risovaca cave and within Vencac massif Shumadies, Serbia

    Directory of Open Access Journals (Sweden)

    Wrzak-Tomić Janina

    2004-01-01

    Full Text Available In the Risovaca cave are found, for the karst, atypical alteration in the limestones structure. Also, morphogenesis of the object can not be logically interpret. Those differences are result of hydrothermal process in initial phase of karstic cycle. And then activity of hot water and hot emanations brought up to the metasomatism and destruction of rock, enormous excrete of ornaments and later, ceiling collapse and fill up of cave room.

  7. Post-speleogenetic biogenic modification of Gomantong Caves, Sabah, Borneo

    Science.gov (United States)

    Lundberg, Joyce; McFarlane, Donald A.

    2012-07-01

    The Gomantong cave system of eastern Sabah, Malaysia, is well-known as an important site for harvesting edible bird-nests and, more recently, as a tourist attraction. Although the biology of the Gomantong system has been repeatedly studied, very little attention has been given to the geomorphology. Here, we report on the impact of geobiological modification in the development of the modern aspect of the cave, an important but little recognized feature of tropical caves. Basic modeling of the metabolic outputs from bats and birds (CO2, H2O, heat) reveals that post-speleogenetic biogenic corrosion can erode bedrock by between ~ 3.0 mm/ka (1 m/~300 ka) and ~ 4.6 mm/ka (1 m/~200 ka). Modeling at high densities of bats yields rates of corrosion of ~ 34 mm/ka (or 1 m/~30 ka). Sub-aerial corrosion creates a previously undescribed speleological feature, the apse-flute, which is semicircular in cross-section and ~ 80 cm wide. It is vertical regardless of rock properties, developing in parallel but apparently completely independently, and often unbroken from roof to floor. They end at a blind hemi-spherical top with no extraneous water source. Half-dome ceiling conch pockets are remnants of previous apse-fluting. Sub-cutaneous corrosion creates the floor-level guano notch formed by organic acid dissolution of bedrock in contact with guano. Speleogenetic assessment suggests that as much as 70-95% of the total volume of the modern cave may have been opened by direct subaerial biogenic dissolution and biogenically-induced collapse, and by sub-cutaneous removal of limestone, over a timescale of 1-2 Ma.

  8. Late quaternary brown bear (Ursidae: Ursus cf. arctos) from a cave in the Huachuca Mountains, Arizona

    Science.gov (United States)

    Nicholas J. Czaplewski; Steve Willsey

    2013-01-01

    In 2008, Steve Willsey discovered the fragmentary cranium of a bear loose on the floor of a cave at about 2270 m elevation near the crest of the Huachuca Mountains. In 2009, we revisited the cave to examine the specimen with the intention of identifying the species. We photographed and measured the main pieces and left them in the cave. The skull is from an adult,...

  9. Cave invertebrates in Espírito Santo state, Brazil: a primary analysis of endemism, threats and conservation priorities

    Directory of Open Access Journals (Sweden)

    Marconi Souza Silva

    2015-08-01

    Full Text Available The cave-dwelling invertebrates were studied according to their composition, biodiversity, distribution and threats in the Atlantic Forest Central Biodiversity Corridor, a priority area for conservation actions in Brazil. Twelve obligate cave species were found, plus 495 troglophile species. Araneae (103 spp., Coleoptera (61 spp., Diptera (56 spp. and Lepidoptera (38 spp. were the richest taxa. The richness was higher in the carbonate caves (63 spp., sd = 16.7 and the highest diversity in granitic caves (H´= 2.68, sd = 0.5. The spatial turnover was 63.45 and similarity less than 30%. The total richness was correlated with the linear extension of the caves (Rs = 0.757, p ≤ 0.05. Surrounding area deforestation and religious and tourist use were the main threats. Emergency attention is recommended regarding protective actions, management and conservation of caves of extremely high biological importance.

  10. Histopathological changes in Poecilia latipinna male gonad due to Tribulus terrestris administration.

    Science.gov (United States)

    Kavitha, P; Ramesh, R; Subramanian, P

    2012-05-01

    Tribulus terrestris is a traditionally known non-toxic aphrodisiac herb for maleness. It was experimented recently to understand the effect and mechanism on mono sex production in Poecilia latipinna. It would help to develop a new eco-friendly way to masculinize P. latipinna, since males have higher commercial value than females. The different concentration (100, 150, 200, 250 and 300 mg/L) of T. terrestris extract and a control were tested for their effect on sex transformation/reversal in P. latipinna by immersing the newly born young ones in the respective concentrations for 2 mo. The obtained results indicate that a dose dependant masculinization is obtained due to T. terrestris administration, which improved the male proportion. Histological results revealed that the testes of fish treated with T. terrestris extract contained all stages of spermatogenesis, clearly demonstrating that the administration of T. terrestris extract to P. latipinna stimulated spermatogenesis. Thus, it is discernible that 0-d-old hatchlings of P. latipinna exposed to T. terrestris extract orient/reverse their sex more towards maleness besides yielding better growth and spermatogenesis which is a mandate for fancy fish industry.

  11. Winter distribution and use of high elevation caves as foraging sites by the endangered Hawaiian hoary bat, Lasiurus cinereus semotus

    Science.gov (United States)

    Bonaccorso, Frank; Montoya-Aiona, Kristina; Pinzari, Corinna A.; Todd, Christopher M.

    2016-01-01

    We examine altitudinal movements involving unusual use of caves by Hawaiian hoary bats, Lasiurus cinereus semotus, during winter and spring in the Mauna Loa Forest Reserve (MLFR), Hawai‘i Island. Acoustic detection of hoary bat vocalizations, were recorded with regularity outside 13 lava tube cave entrances situated between 2,200 to 3,600 m asl from November 2012 to April 2013. Vocalizations were most numerous in November and December with the number of call events and echolocation pulses decreasing through the following months. Bat activity was positively correlated with air temperature and negatively correlated with wind speed. Visual searches found no evidence of hibernacula nor do Hawaiian hoary bats appear to shelter by day in these caves. Nevertheless, bats fly deep into caves as evidenced by numerous carcasses found in cave interiors. The occurrence of feeding buzzes around cave entrances and visual observations of bats flying in acrobatic fashion in cave interiors point to the use of these spaces as foraging sites. Peridroma moth species (Noctuidae), the only abundant nocturnal, flying insect sheltering in large numbers in rock rubble and on cave walls in the MLFR, apparently serve as the principal prey attracting hoary bats during winter to lava tube caves in the upper MLFR. Caves above 3,000 m on Mauna Loa harbor temperatures suitable for Pseudogymnoascus destructansfungi, the causative agent of White-nose Syndrome that is highly lethal to some species of North American cave-dwelling bats. We discuss the potential for White-nose Syndrome to establish and affect Hawaiian hoary bats.

  12. Bat ticks revisited: Ixodes ariadnae sp. nov. and allopatric genotypes of I. vespertilionis in caves of Hungary.

    Science.gov (United States)

    Hornok, Sándor; Kontschán, Jenő; Kováts, Dávid; Kovács, Richárd; Angyal, Dorottya; Görföl, Tamás; Polacsek, Zsolt; Kalmár, Zsuzsa; Mihalca, Andrei D

    2014-04-27

    In Europe two ixodid bat tick species, Ixodes vespertilionis and I. simplex were hitherto known to occur. Bat ticks were collected from cave walls and bats in Hungary. Their morphology and genotypes were compared with microscopy and conventional PCR (followed by sequencing), respectively. A year-round activity of I. vespertilionis was observed. Molecular analysis of the cytochrome oxidase subunit I (COI) gene of twenty ticks from different caves showed that the occurrence of the most common genotype was associated with the caves close to each other. A few specimens of a morphologically different tick variant were also found and their COI analysis revealed only 86-88% sequence homology with I. simplex and I. vespertilionis, respectively. The microenvironment of caves (well separated from each other) appears to support the existence of allopatric I. vespertilionis COI genotypes, most likely related to the distance between caves and to bat migration over-bridging certain caves. The name I. ariadnae sp. nov. is given to the new tick species described here for the first time.

  13. The Impact of Geomorphology and Human Disturbances on the Faunal Distributions in Tiquara and Angico Caves of Campo Formoso, Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    André Vieira de Araujo

    2015-04-01

    Full Text Available Characterization of fauna is important for the understanding of communities and ecosystems, enabling the design of actions for conservation. In the present piece of work, we identified total 45 morphospecies belonging to the order Acarina, Pseudoscorpionida, Dictyoptera, Araneida, Amblypygi, Isopoda, Plecoptera, Amphipoda, Zygentoma, Spirostreptida, Coleoptera, Collembola, Diptera, Ensifera, Heteroptera, Hymenoptera, Lepidoptera and Psocoptera from two distinct types of caves of Campo Formoso, state of Bahia in Brazil. It was made to provide subsidies for conservation studies. The targeted caves were Tiquara Cave suffered for many years from saltpeter extraction and Angico Cave less visited cave having high tourist potential. Though the conservation status is much better in Angico cave, but we found comparatively more morphospecies in Tiquara cave.

  14. Mimogonellus dreybrodti sp. n., a new cave-inhabiting Osoriinae from Laos (Coleoptera: Staphylinidae).

    Science.gov (United States)

    Yin, Zi-Wei; Steiner, Helmut

    2017-10-17

    A new osoriine species, Mimogonellus dreybrodti Yin & Steiner, sp. n., collected from a cave in Houaphanh Province, Laos, is described and illustrated. This represents the third Mimogonellus species in Asia, and the first in the genus known to inhabit a cave environment.

  15. 222 Rn exposure assessment in the caves of Parque Estadual Turistico do Alto Ribeira (PETAR)

    International Nuclear Information System (INIS)

    Alberigi, Simone

    2006-01-01

    In the present work, radon concentrations in six caves of PETAR - Parque Estadual Turistico do Alto Ribeira (High Ribeira River Touristic State Park) were carried out with Makrofol E solid state nuclear track detectors (SSNTD) and used to assess the annual effective dose received by regional tour guides. The park has four visitation centers: Santana, Ouro Grosso, Caboclos e Casa de Pedra and receives nearly 40,000 people annually. The caves evaluated were Couto, Agua Suja, Laje Branca, Morro Preto and Santana, from Santana center and Alambari de Baixo from Ouro Grosso center, for being the most frequently visited caves. The exposure period of the SSNTD was, at least, three months, over a period of 26 months, from October 2003 to November 2005.The 222 Rn concentrations lay in a range from 153 Bq.m -3 to 6607 Bq.m -3 and we observed that, in general, for chilly weather, the radon levels decrease. The annual effective dose, considering the most realistic scenario, with geometric mean concentrations, an equilibrium factor of 0.5 and annual exposure time for each cave, varied from 0.2 mSv.a -1 for the Couto cave, strongly ventilated, to 4.0 mSv.a -1 for the Santana cave, the most frequently visited and no external communication. For the worst scenario, with arithmetic mean concentrations, equilibrium factor 1 and annual exposure time for all caves, the annual effective dose was 16.1 mSv.a -1 . All assessed effective doses received by the tour guides are bellow 20 mSv.a -1 suggested as an annual effective dose limit for occupational exposure by the International Commission of Radiological Protection (ICRP 60, 1990). (author)

  16. Food sources of selected terrestrial cave arthropods

    Czech Academy of Sciences Publication Activity Database

    Smrž, J.; Kováč, L.; Mikeš, J.; Šustr, Vladimír; Lukešová, Alena; Tajovský, Karel; Nováková, Alena; Režňáková, P.

    2015-01-01

    Roč. 16, č. 1 (2015), s. 37-46 ISSN 1768-1448 Grant - others:Vega(SK) 1/0139/09 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Acari * caves * Collembola * Diplopoda * feeding habits * Isopoda Subject RIV: EG - Zoology

  17. Late Quaternary speleogenesis and landscape evolution in a tropical carbonate island: Pango la Kuumbi (Kuumbi Cave, Zanzibar

    Directory of Open Access Journals (Sweden)

    Nikos Kourampas

    2015-09-01

    Full Text Available Kuumbi Cave is one of a group of caves that underlie a flight of marine terraces in Pleistocene limestone in eastern Zanzibar (Indian Ocean. Drawing on the findings of geoarchaeological field survey and archaeological excavation, we discuss the formation and evolution of Kuumbi Cave and its wider littoral landscape. In the later part of the Quaternary (last ca. 250,000 years?, speleogenesis and terrace formation were driven by the interplay between glacioeustatic sea level change and crustal uplift at rates of ca. 0.10-0.20 mm/yr. Two units of backreef/reef limestone were deposited during ‘optimal’ (highest highstands, tentatively correlated with MIS 7 and 5; (mainly erosive marine terraces formed in these limestones in ‘suboptimal’ highstands. Kuumbi and other sub-terrace caves developed as flank margin caves, in the seaward portion of freshwater lenses during such ‘suboptimal’ highstands. Glacioeustacy-induced fluctuations of the groundwater table may have resulted in shifts from vadose (with deposition of well-developed speleothems to phreatic/epiphreatic conditions in these caves. At Kuumbi, Late Pleistocene (pre-20,000 cal. BP ceiling collapse initiated colluvial deposition near-entrance and opened the cave to large plants and animals, including humans. A phase of terminal Pleistocene human occupation ca. 18,500-17,000 cal. BP resulted in the deposition of a dense assemblage of Achatina spp. landsnails, alongside marine molluscs and mammal remains (including zebra, buffalo and other taxa now extinct on Zanzibar. The Holocene part of the cave stratigraphy near-entrance records phases of abandonment and intensified late Holocene human use.

  18. Solar-forced diurnal regulation of cave drip rates via phreatophyte evapotranspiration

    Directory of Open Access Journals (Sweden)

    K. Coleborn

    2016-11-01

    Full Text Available We present results of a detailed study of drip rate variations at 12 drip discharge sites in Glory Hole Cave, New South Wales, Australia. Our novel time series analysis, using the wavelet synchrosqueezed transform, reveals pronounced oscillations at daily and sub-daily frequencies occurring in 8 out of the 12 monitored sites. These oscillations were not spatially or temporally homogenous, with different drip sites exhibiting such behaviour at different times of year in different parts of the cave. We test several hypotheses for the cause of the oscillations, including variations in pressure gradients between karst and cave due to cave breathing effects or atmospheric and earth tides, variations in hydraulic conductivity due to changes in viscosity of water with daily temperature oscillations, and solar-driven daily cycles of vegetative (phreatophytic transpiration. We conclude that the only hypothesis consistent with the data and hydrologic theory is that daily oscillations are caused by solar-driven pumping by phreatophytic trees which are abundant at the site. The daily oscillations are not continuous and occur sporadically in short bursts (2–14 days throughout the year due to non-linear modification of the solar signal via complex karst architecture. This is the first indirect observation leading to the hypothesis of tree water use in cave drip water. It has important implications for karst hydrology in regards to developing a new protocol to determine the relative importance of trends in drip rate, such as diurnal oscillations, and how these trends change over timescales of weeks to years. This information can also be used to infer karst architecture. This study demonstrates the importance of vegetation on recharge dynamics, information that will inform both process-based karst models and empirical estimation approaches. Our findings support a growing body of research exploring the impact of trees on speleothem paleoclimate proxies.

  19. Phlebotomines (Diptera, Psychodidae in caves of the Serra da Bodoquena, Mato Grosso do Sul State, Brazil

    Directory of Open Access Journals (Sweden)

    Eunice A. B. Galati

    2003-01-01

    Full Text Available The present paper deals with the phlebotomine species captured during the period from January 1998 to June 2000 in 12 caves located in the Serra da Bodoquena, situated in the south central region of Mato Grosso do Sul State, Brazil. Three of the caves are situated further north (in Bodoquena county, seven in the central area (Bonito county and two in the south (Jardim county. These last two caves and three of those in Bonito are located at the west side of the ridge. Eighteen species of phlebotomines were captured within the caves: Brumptomyia avellari (Costa Lima, 1932, Brumptomyia brumpti (Larrousse, 1920, Brumptomyia cunhai (Mangabeira, 1942, Brumptomyia galindoi (Fairchild & Hertig, 1947, Evandromyia corumbaensis (Galati, Nunes, Oshiro & Rego, 1989, Lutzomyia almerioi Galati & Nunes, 1999, Lutzomyia longipalpis (Lutz & Neiva, 1912, Martinsmyia oliveirai (Martins, Falcão & Silva, 1970, Micropygomyia acanthopharynx (Martins, Falcão & Silva, 1962, Micropygomyia peresi (Mangabeira, 1942, Micropygomyia quinquefer (Dyar, 1929, Nyssomyia whitmani (Antunes & Coutinho, 1939, Psathyromyia campograndensis (Oliveira, Andrade-Filho, Falcão & Brazil, 2001, Psathyromyia punctigeniculata (Floch & Abonnenc, 1944, Psathyromyia shannoni (Dyar, 1929, Pintomyia kuscheli (Le Pont, Martinez, Torrez-Espejo & Dujardin, 1998, Sciopemyia sordellii (Shannon & Del Ponte, 1927 and Sciopemyia sp. A total of 29,599 phlebotomine sandflies was obtained. Lutzomyia almerioi was absolutely predominant (91.5% over the other species on both sides of the Bodoquena ridge, with the exception of the southern caves in which it was absent. It presents summer predominance, with nocturnal and diurnal activities. The species breeds in the caves and was captured during daytime both in the dark area and in the mouth of the caves. Martinsmyia oliveirai, the second most frequent sandfly, also presents a summer peak and only predominated over the other species in one cave, in which there

  20. Wintering bats of the upper Snake River Plain: occurrence in lava-tube caves

    Energy Technology Data Exchange (ETDEWEB)

    Genter, D.L.

    1986-04-30

    Distribution and habitat selection of hibernating bats at the Idaho National Engineering Laboratory (INEL) and adjacent area are reported. Exploration of over 30 lava-tube caves revealed that two species, Myotis leibii and Plecotus townsendii, hibernate in the upper Snake River Plain. Five species, M. lucifugus, M. evotis, Eptesicus fuscus, Lasionycteris noctivagans, and Lasiurus cinereus are considered migratory. Myotis leibii and P. townsendii hibernate throughout much of the area, occasionally in mixed-species groups. Myotis leibii uses the dark and protected regions of the cave, usually wedged into tiny pockets and crevices near or at the highest portion of the ceiling. Individuals of P. townsendii may be found at any height or depth in the cave. Temperature appears to be primary limiting factor in habitat selection. Myotis leibii was found in significantly cooler air temperatures than P. townsendii. Neither species tolerated continuous temperatures below 1.5 C. Relative humidity does not seem to be a significant factor in the distribution or habitat selection of the two species in lava-tube caves. 18 references, 1 figure, 1 table.

  1. Microbial communities in a coastal cave: Cova des Pas de Vallgornera (Mallorca, Western Mediterranean

    Directory of Open Access Journals (Sweden)

    Antoni Busquets

    2014-05-01

    Full Text Available As a part of an ongoing project on the role of microbes in the biogeochemistry of Majorcan caves, the species diversity of microbial communities present in cave pools of anchialine waters in the Cova des Pas de Vallgornera (Mallorca, western Mediterranean is investigated by a culture-dependent method. Two-hundred and forty-eight strains isolated from this characteristic cave environment of the littoral karst are identified by whole-cell-MALDI-TOF mass spectrometry and phylogeneticaly by 16S rRNA gene sequences. Total cell counts and species diversity of the bacterial communities decreas with the distance to the entrance of the cave and to the sea. Strains are mainly identified as members of the Gammaproteobacteria and Actinobacteria. Around 20% of the isolates are able to precipitate carbonates. Calcite is the predominant phase, growing in all the precipitates, although struvite is also found in one Pseudomonas and in one Aspergillus cultures. Differences in crystal characteristics of external shape (habit and growth are observed according to the bacterial species promoting the precipitates. Bacteria associated with multicolored ferromanganese deposits, present in several parts of the cave, are also studied and are identified as Pseudomonas benzenivorans and Nocardioides luteus. The preponderance of Pseudomonas species and the possible contribution of bacteria in calcite deposition are discussed.

  2. DIGITAL PRESERVATION OF ANCIENT MAYA CAVE ARCHITECTURE: RECENT FIELD EFFORTS IN QUINTANA ROO, MEXICO

    Directory of Open Access Journals (Sweden)

    D. Rissolo

    2017-08-01

    Full Text Available The presence of ancient Maya shrines in caves serves as unequivocal evidence for the ritual appropriation of these subterranean spaces and their significance with respect to Maya religious practice. Detailed study of the miniature masonry temples and altar features in the caves of Quintana Roo, Mexico reveals a strong stylistic and likely functional correspondence between these structures and their terrestrial counterparts at Postclassic sites. The Proyecto Arquitectura Subterranea de Quintana Roo (coordinated by the Center of Interdisciplinary Science for Art, Architecture, and Archaeology, or CISA3, at the University of California, San Diego and in collaboration with the Instituto Nacional de Antropologia e Historia in Mexico is conducting a survey and program of digital documentation of both the pristine and impacted cave shrines of the region. Once an area is developed and populated, and access is opened to caves containing ancient architectural features, they are soon vandalized – often resulting in the complete obliteration of these rare miniature buildings and their diagnostic architectural elements. This emergent situation necessitates the use of rapid reality-capture tools; however, the physical challenges of working in caves requires researchers of adapt increasingly common architectural documentation methodologies to more adverse field conditions.

  3. Age and speleogenesis of epigenic gypsum caves in the northern Apennines (Italy)

    Science.gov (United States)

    Columbu, Andrea; Chiarini, Veronica; De Waele, Jo; Drysdale, Russell; Forti, Paolo; Hellstrom, John; Woodhead, Jon

    2016-04-01

    Triassic and Messinian gypsum beds host the majority of the caves in the eastern flank of the northern Apennines. To date, more than six hundreds voids have been mapped, including the longest known epigenic gypsum cave system in the world (Spipola-Acquafredda, ~11 km of tunnels) (De Waele et al., 2013). Superimposed caves are typically sub-horizontal (Klimchouk, 2000) and connected through vertical shafts, reflecting the palaeo base-level variations. When preserved, river terraces at the surface lie at the same palaeo altitude of the base level and horizontal cave passages. Notwithstanding the well-known geology of the area known (Vai and Martini, 2001), the age of these caves has been greatly underestimated in the past. Considering the rapid dissolution of the gypsum and uplifting of the area, the start of speleogenesis activity was considered to have occurred during the last glacial age. The age of karst voids can be only indirectly estimated by the dating of the infilling sediments. U-Th dating on carbonate speleothems provides high-precision and accurate ages (Hellstrom, 2003; Scholz and Hoffmann, 2008). We thus applied this methodology to 20 speleothems coming from 14 different caves belonging to the Monte Tondo, Spipola Acquafredda, Castelnuovo, Stella-Rio Basino and Brisighella systems. The results show that: i) caves were forming since at least ~300 ka; ii) the peak of speleogenesis was reached during relatively cold climate stages, when rivers formed terraces at the surface and aggradation caused paragenesis in the stable cave levels (Columbu et al., 2015). Besides the significant contribution to the understanding of the Apennines evaporite karst evolution, this study (and its further advancement) may also refine knowledge of the local vs regional uplifting rates and base-level variations since the late Pleistocene (Wegmann and Pazzaglia, 2009). References Columbu, A., De Waele, J., Forti, P., Montagna, P., Picotti, V., Pons-Branchu, E., Hellstrom, J

  4. Malaria Disease Mapping in Malaysia based on Besag-York-Mollie (BYM) Model

    Science.gov (United States)

    Azah Samat, Nor; Mey, Liew Wan

    2017-09-01

    Disease mapping is the visual representation of the geographical distribution which give an overview info about the incidence of disease within a population through spatial epidemiology data. Based on the result of map, it helps in monitoring and planning resource needs at all levels of health care and designing appropriate interventions, tailored towards areas that deserve closer scrutiny or communities that lead to further investigations to identify important risk factors. Therefore, the choice of statistical model used for relative risk estimation is important because production of disease risk map relies on the model used. This paper proposes Besag-York-Mollie (BYM) model to estimate the relative risk for Malaria in Malaysia. The analysis involved using the number of Malaria cases that obtained from the Ministry of Health Malaysia. The outcomes of analysis are displayed through graph and map, including Malaria disease risk map that constructed according to the estimation of relative risk. The distribution of high and low risk areas of Malaria disease occurrences for all states in Malaysia can be identified in the risk map.

  5. Speleoclimate dynamics in Santana Cave (PETAR, São Paulo State, Brazil: general characterization and implications for tourist management

    Directory of Open Access Journals (Sweden)

    Heros Augusto Santos Lobo

    2015-01-01

    Full Text Available Show caves provide tourists with the opportunity to have close contact with natural underground spaces. However, visitation to these places also creates a need for management measures, mainly the definition of tourist carrying capacity. The present work describes the results of climate monitoring and atmospheric profiling performed in Santana Cave (Alto Ribeira State and Tourist Park – PETAR, Brazil between 2008 and 2011. Based on the results, distinct preliminary zones with different levels of thermal variation were identified, which classify Santana Cave as a warm trap. Two critical points along the tourist route (Cristo and Encontro Halls were identified where the temperature of the locality increased by 1.3 °C when tourists were present. Air flow from the inner cave to the outside occurs during the austral summer, and the opposite flow occurs when the outside environment is colder than the air inside the cave during the austral winter. The temperature was used to establish thresholds to the tourist carrying capacity by computing the recovery time of the atmospheric conditions after the changes caused by the presence of tourists. This method suggests a maximum limit of approximately 350 visits per day to Santana Cave. The conclusion of the study is that Santana Cave has an atmosphere that is highly connected with the outside; daily variations in temperature and, to a lesser extent, in the relative humidity occur throughout the entire studied area of the cave. Therefore, the tourist carrying capacity in Santana Cave can be flexible and can be implemented based on the climate seasonality, the tourism demand and other management strategies.

  6. Cultivo de Poecilia reticulata (Pisces:Poecilidae en cuerpos de agua tropicales,Veracruz,México

    Directory of Open Access Journals (Sweden)

    Patricia Devezé Murillo

    2004-12-01

    Full Text Available Los peces ornamentales cultivados se han popularizado como mascotas,generando una demanda local que no se ha podido cubrir con la producción de México y por lo tanto deben importarse.Poecilia reticulata "guppy ",es un pez muy popular en el ámbito acuarístico,hoy en día se ha logrado desarrollar una gran cantidad de variedades según su coloración,tipo y forma de cola.Son peces vivíparos que toleran un ámbito extremo de temperatura,siendo la más adecuada entre 25ºC y 28ºC.El tiempo que tardan las hembras en expulsar a las crías una vez que quedan preñadas,oscila entre los 25 y 30 días dependiendo de la temperatura.En esta investigación,el ciclo reproductivo completo se desarrolló en jaulas flotantes,en una laguna tropical situada en la Llanura Costera del Golfo Sur, Veracruz, México.Se diseñó la infraestructura necesaria para producir guppys (P.reticulata incluyendo el manejo de reproductores,crianza,engorda,alimentación y comercialización,evaluando su factibilidad técnica y financiera.Se realizó un estudio de mercado,a través de una encuesta aplicada a los propietarios de los 22 acuarios existentes en la Ciudad de Veracruz,México.Se analizaron las frecuencias de oferta y demanda de cada especie con respecto a la temporada del año,lugar de origen y resistencia al manejo.Las características hidrobiológicas presentaron valores medios de temperatura (31.4ºC,oxígeno disuelto (4.5 mgl-1y pH (6.8.Con los datos obtenidos en campo se realizó un modelo para producción de P.reticulata ,obteniendo una relación beneficio/costo=1.16,correspondiente al ingreso mínimo potencial con una ganancia bruta de US$257.67, proporcionando empleo a dos personas El aprovechamiento de cuerpos de agua en regiones tropicales a través de la producción de peces de ornato representa una alternativa para disminuir la presión sobre las poblaciones silvestres y las tierras de cultivo,proporcionar fuentes de empleo e incrementar los ingresos para

  7. Self-Reported Ecstasy/MDMA/"Molly" Use in a Sample of Nightclub and Dance Festival Attendees in New York City.

    Science.gov (United States)

    Palamar, Joseph J; Acosta, Patricia; Ompad, Danielle C; Cleland, Charles M

    2017-01-02

    Ecstasy (MDMA) use has regained popularity in the United States, particularly in the form of "Molly," which is often marketed as pure MDMA. Surveys have generally not included "Molly" in the definition of ecstasy, so rates of use may be underestimated. As popularity of ecstasy increases, research is needed to examine use among those at highest risk for use-nightlife attendees. We surveyed 679 young adults (age 18-25) entering nightclubs and festivals holding electronic dance music (EDM) parties in New York City in 2015. A variation of time-space sampling was utilized. We examined prevalence and correlates of self-reported lifetime ecstasy use. Self-reported lifetime ecstasy use was common (42.8%, 95% CI: 32.8, 52.7). Use was most common among older participants, frequent party attendees, and those reporting higher levels of exposure to users. Those surveyed outside of festivals were less likely to report use compared to those surveyed outside of nightclubs (AOR = 0.37, p = .015). Over a third of ecstasy users (36.8%)reported use in pill, powder, and crystal form. Ecstasy users were also more likely to report use of other drugs, including novel psychoactive substances (e.g., 2C series drugs, synthetic cathinones ["bath salts"]). Half (50.4%) reported suspecting (21.9%) or finding out (28.5%) that their ecstasy had ever contained a drug other than MDMA. A large percentage of nightlife attendees in NYC report lifetime ecstasy use. Findings should inform prevention and harm reduction programming. Further research is needed as ecstasy continues to change (e.g., in form, purity, and name).

  8. Behavioral response of cave and surface Asellus aquaticus to water current

    OpenAIRE

    Dacar, Maja

    2017-01-01

    There are many questions regarding what influences the emergence of new species. Firstly and above all, is the appearance of differences within a certain specie, where a certain part is isolated from the group and continues its own evolution. One of these differences appear between the surface- and cave-dwelling Asellus aquaticus, as the ability to hold on to their surface. The discovery of these differences was carried out using a method of experiment, namely on the cave-dwelling Asellus ...

  9. Asymmetric Meckel Cave Enlargement: A Potential Marker of PHACES Syndrome.

    Science.gov (United States)

    Wright, J N; Wycoco, V

    2017-06-01

    PHACES syndrome is a complex of morphologic abnormalities of unknown cause and includes posterior fossa abnormalities; head and neck infantile hemangiomas; arterial, cardiac, and eye anomalies; and sternal or abdominal wall defects. Accurate identification of the syndrome is important for optimal treatment. The purpose of this study was to investigate the incidence of asymmetric Meckel cave enlargement, a potential novel imaging marker, in a population of patients referred for evaluation of possible PHACES syndrome. Eighty-five patients referred for neuroimaging evaluation of possible PHACES syndrome were identified and stratified on the basis of their ultimate clinical PHACES diagnosis categorization into PHACES, possible PHACES, or not PHACES. MR imaging studies were subsequently reviewed for the presence or absence of unilateral Meckel cave enlargement, with the reviewer blinded to the ultimate PHACES syndrome categorization. Twenty-five of 85 patients (29%) were ultimately categorized as having PHACES or possible PHACES according to consensus guidelines. Asymmetric Meckel cave enlargement was present in 76% (19/25) of these patients and in 82% (19/23) of only those patients with definite PHACES. This finding was present in none of the 60 patients determined not to have PHACES syndrome. In 7/19 patients (37%) with this finding, subtle MR imaging abnormalities consistent with PHACES were missed on the initial MR imaging interpretation. Asymmetric Meckel cave enlargement was a common feature of patients with PHACES in our cohort and may serve as a novel imaging marker. Increased awareness of this imaging feature has the potential to increase the diagnostic accuracy of PHACES. © 2017 by American Journal of Neuroradiology.

  10. Direct radiocarbon dates for prehistoric paintings at the Altamira, El Castillo and Niaux caves

    International Nuclear Information System (INIS)

    Valladas, H.; Cachier, H.; Maurice, P.; Arnold, M.; Quiros, F.B. de; Valdes, V.C.; Uzquiano, P.

    1992-01-01

    Among things that most strikingly distinguish modern humans from other hominids and the rest of the animal kingdom is the ability to represent things and events pictorially. Complex paintings of the type discovered in the Altamira, El Castillo, Niaux and Lascaux caves represent an important stepping stone in the cultural evolution of humankind. Until now dates were derived from style or dated remains left by prehistoric visitors and could be biased by prolonged occupation or visits unrelated to painting activity. Here we report the first radiocarbon dates for the charcoal used to draw stylistically similar bisons in these caves: 14,000 ± 400 yr BP in the Spanish caves of Altamira, 12,990 ± 200 yr BP in El Castillo, and 12,890 ± 160 yr BP for a bison of different style in the French Pyrenean cave of Niaux. Our results demonstrate the imprecise nature of stylistic dating and show that painting dates derived from remains of human activities should be used with caution. (Author)

  11. Geomorphometric analysis of cave ceiling channels mapped with 3-D terrestrial laser scanning

    Science.gov (United States)

    Gallay, Michal; Hochmuth, Zdenko; Kaňuk, Ján; Hofierka, Jaroslav

    2016-05-01

    The change of hydrological conditions during the evolution of caves in carbonate rocks often results in a complex subterranean geomorphology, which comprises specific landforms such as ceiling channels, anastomosing half tubes, or speleothems organized vertically in different levels. Studying such complex environments traditionally requires tedious mapping; however, this is being replaced with terrestrial laser scanning technology. Laser scanning overcomes the problem of reaching high ceilings, providing new options to map underground landscapes with unprecedented level of detail and accuracy. The acquired point cloud can be handled conveniently with dedicated software, but applying traditional geomorphometry to analyse the cave surface is limited. This is because geomorphometry has been focused on parameterization and analysis of surficial terrain. The theoretical and methodological concept has been based on two-dimensional (2-D) scalar fields, which are sufficient for most cases of the surficial terrain. The terrain surface is modelled with a bivariate function of altitude (elevation) and represented by a raster digital elevation model. However, the cave is a 3-D entity; therefore, a different approach is required for geomorphometric analysis. In this paper, we demonstrate the benefits of high-resolution cave mapping and 3-D modelling to better understand the palaeohydrography of the Domica cave in Slovakia. This methodological approach adopted traditional geomorphometric methods in a unique manner and also new methods used in 3-D computer graphics, which can be applied to study other 3-D geomorphological forms.

  12. Secondary sulfate minerals from Alum Cave Bluff: Microscopy and microanalysis

    Energy Technology Data Exchange (ETDEWEB)

    Lauf, R.J.

    1997-07-01

    Microcrystals of secondary sulfate minerals from Alum Cave Bluff, Great Smoky Mountains National Park, were examined by scanning electron microscopy and identified by X-ray fluorescence (XRF) in the SEM. Among the samples the author discovered three new rare-earth sulfates: coskrenite-(Ce), levinsonite-(Y), and zugshunstite-(Ce). Other minerals illustrated in this report include sulfur, tschermigite, gypsum, epsomite, melanterite, halotrichite, apjohnite, jarosite, slavikite, magnesiocopiapite, and diadochite. Additional specimens whose identification is more tentative include pickeringite, aluminite, basaluminite, and botryogen. Alum Cave is a ``Dana locality`` for apjohnite and potash alum, and is the first documented North American occurrence of slavikite.

  13. Three new cave-dwelling trechine ground beetles from eastern and southeastern Serbia (Coleoptera: Carabidae: Trechinae

    Directory of Open Access Journals (Sweden)

    Ćurčić S.B.

    2014-01-01

    Full Text Available Three new troglobitic trechine ground beetle species are described from three caves in eastern and southeastern Serbia: Duvalius (Paraduvalius bogovinae sp. n., from the Bogovinska Pećina Cave, village of Bogovina, Kučajske Planine Mts., near Boljevac, eastern Serbia; D. (P. milutini sp. n., from the Samar cave system, village of Kopajkošara, Mt. Kalafat, near Svrljig, southeastern Serbia, and D. (P. beljanicae sp. n., from the Velika Atula Cave, village of Strmosten, Mt. Beljanica, near Despotovac, eastern Serbia. The new species are easily distinguished from relatives. All important morphological features, along with the diagnoses and illustrations of the new taxa are presented. The new species are relicts and endemics of eastern and southeastern Serbia. They probably belong to old phyletic lineages of Tertiary or even pre-Tertiary origin. [Projekat Ministarstva nauke Republike Srbije, br. 173038

  14. Comparative study of acetylcholinesterase and glutathione S-transferase activities of closely related cave and surface Asellus aquaticus (Isopoda: Crustacea.

    Directory of Open Access Journals (Sweden)

    Anita Jemec

    Full Text Available The freshwater isopod crustacean Asellus aquaticus has recently been developed as an emerging invertebrate cave model for studying evolutionary and developmental biology. Mostly morphological and genetic differences between cave and surface A. aquaticus populations have been described up to now, while scarce data are available on other aspects, including physiology. The purpose of this study was to advance our understanding of the physiological differences between cave A. aquaticus and its surface-dwelling counterparts. We sampled two surface populations from the surface section of the sinking Pivka River (central Slovenia, Europe, i.e. locality Pivka Polje, and locality Planina Polje, and one cave population from the subterranean section of the sinking Pivka River, i.e. locality Planina Cave. Animals were sampled in spring, summer and autumn. We measured the activities of acetylcholinesterase (AChE and glutathione S-transferase (GST in individuals snap-frozen in the field immediately after collection. Acetylcholinesterase is likely related to animals' locomotor activity, while GST activity is related to the metabolic activity of an organism. Our study shows significantly lower AChE and GST activities in the cave population in comparison to both surface A. aquaticus populations. This confirms the assumption that cave A. aquaticus have lower locomotor and metabolic activity than surface A. aquaticus in their respective natural environments. In surface A. aquaticus populations, seasonal fluctuations in GST activity were observed, while these were less pronounced in individuals from the more stable cave environment. On the other hand, AChE activity was generally season-independent in all populations. To our knowledge, this is the first study of its kind conducted in A. aquaticus. Our results show that among closely related cave and surface A. aquaticus populations also physiological differences are present besides the morphological and genetic

  15. The mass and energy balance of ice within the Eisriesenwelt cave, Austria

    Directory of Open Access Journals (Sweden)

    F. Obleitner

    2011-03-01

    Full Text Available Meteorological measurements were performed in a prominent ice cave (Eisriesenwelt, Austria during a full annual cycle. The data show the basic features of a dynamically ventilated cave system with a well distinguished winter and summer regime.

    The calculated energy balance of the cave ice is largely determined by the input of long-wave radiation originating at the host rock surface. On average the turbulent fluxes withdraw energy from the surface. This is more pronounced during winter due to enhanced circulation and lower humidity. During summer the driving gradients reverse sign and the associated fluxes provide energy for melt.

    About 4 cm of ice were lost at the measurement site during a reference year. This was due to some sublimation during winter, while the major loss resulted from melt during summer. Small amounts of accumulation occurred during spring due to refreezing of seepage water.

    These results are largely based on employing a numerical mass and energy balance model. Sensitivity studies prove reliability of the calculated energy balance regarding diverse measurement uncertainties and show that the annual mass balance of the ice strongly depends on cave air temperature during summer and the availability of seepage water in spring.

  16. Dietary ecology of the extinct cave bear: Evidence of omnivory as inferred from dental microwear textures

    OpenAIRE

    D. Brent Jones; Larisa R.G. DeSantis

    2016-01-01

    The diet of the extinct European cave bear, Ursus spelaeus, has widely been debated. Diverging from the extant brown bear (Ursus arctos) approximately 1.2 million years ago, the cave bear is one of the most ubiquitous fossil bears occurring in Europe during the middle and Late Pleistocene. Early morphological studies suggested that the cave bear was likely specialized on processing tough and/or abrasive foods, while later two-dimensional low-magnification microwear studies suggested that they...

  17. Estimation of effective doses to cavers based on radon measurements carried out in seven caves of the Bakony Mountains in Hungary

    International Nuclear Information System (INIS)

    Kavasi, Norbert; Somlai, Janos; Szeiler, Gabor; Szabo, Balazs; Schafer, Istvan; Kovacs, Tibor

    2010-01-01

    Nowadays, as the practice of extreme sports is spreading, potholing is becoming more and more popular. As a result, both the number of cavers and the time spent in the caves have been on the rise. There are some cavers known to have spent some 5000 h in caves over a span of 10 years. In poorly ventilated caves, radon exhalated from cave rocks and deposits may accumulate and cause significant doses to cavers. In this study, the radon concentration in seven caves in the Bakony Mountains, Hungary, was measured by continuous and integrated measurement devices. Measured values for the different caves were rather different, and varied between 50 and 24,000 Bq m -3 . The average radon concentration over the measurement period was approximately 10,000 Bq m -3 in five of the seven caves inspected. By assuming an average of 470 h year -1 spent in caves, effective doses to cavers were estimated. The expected annual effective dose, in case of an equilibrium factor of 0.6, was 19.7 mSv.

  18. Accumulation of dieldrin in an alga (Scenedesmus obliquus), Daphnia magna, and the guppy (Poecilia reticulata)

    Science.gov (United States)

    Reinert, Robert E.

    1972-01-01

    Scenedesmus obliquus, Daphnia magna, and Poecilia reticulata accumulated dieldrin directly from water; average concentration factors (concentration in organism, dry weight, divided by concentration in water) were 1282 for the alga, 13,954 for D. magna, and 49,307 (estimated) for the guppy. The amount accumulated by each species at equilibrium (after about 1.5, 3-4, and 18 days, respectively) was directly proportional to the concentration of dieldrin in the water. Daphnia magna and guppies accumulated more dieldrin from water than from food that had been exposed to similar concentrations in water. When guppies were fed equal daily rations of D. magna containing different concentrations of insecticide, the amounts of dieldrin accumulated by the fish were directly proportional to the concentration in D. magna; when two lots of guppies were fed different quantities of D. magna (10 and 20 organisms per day) containing identical concentrations of dieldrin, however, the amounts accumulated did not differ substantially.

  19. Bullita cave system, Judbarra / Gregory Karst, tropical Australia; Sistemas de cuevas de Bullita, Judbarra / Gregory Karst, Australia tropical

    Energy Technology Data Exchange (ETDEWEB)

    Grimes, K. G.; Martini, J. E. J.

    2016-07-01

    In the monsoon tropics of northern Australia, Bullita Cave is the largest (123 km) of a group of extensive, horizontal, joint-controlled, dense network maze caves which are epikarst systems lying at shallow depth beneath a well-developed karrenfield. The Judbarra / Gregory Karst and its caves are restricted to the outcrop belt of the thin, sub-horizontal, Proterozoic Supplejack Dolostone. Karst is further restricted to those parts of the Supplejack that have escaped a secondary dolomitisation event. The karrenfield and underlying cave system are intimately related and have developed in step as the Supplejack surface was exposed by slope retreat. Both show a lateral zonation of development grading from youth to old age. Small cave passages originate under the recently exposed surface, and the older passages at the trailing edge become un roofed or destroyed as the, by then deeply-incised, karrenfield breaks up into isolated ruiniform blocks and pinnacles. Vertical development of the cave has been generally restricted to the epikarst zone by a 3m bed of impermeable and incompetent shale beneath the Supplejack which first perched the water-table, forming incipient phreatic passages above it, and later was eroded by vadose flow to form an extensive horizontal system of passages 10-20m below the karren surface. Some lower cave levels in underlying dolostone occur adjacent to recently incised surface gorges. Speleogenesis is also influenced by the rapid, diffuse, vertical inflow of storm water through the karrenfield, and by ponding of the still-aggressive water within the cave during the wet season dammed up by levees of sediment that accumulate beneath the degraded trailing edge of the karrenfield. The soil, and much biological activity, is not at the bare karren surface, but down on the cave floors, which aids epikarstic solution at depth rather than on the surface. (Author)

  20. Electronic microscopy and EDX characterization of teotihuacan prehispanic mortar from the cave under the sun pyramid

    Energy Technology Data Exchange (ETDEWEB)

    Martinez, T. [Faculty of Chemistry, National University of Mexico, Building D, CU (O4510) Mexico, D.F. (Mexico)]. E-mail: tmc@servidor.unam.mx; Martinez, G. [Coordinacion Nacional de Conservacion del Patrimonio Cultural. Xicontencatl y General Anaya s/n. (04120) Mexico, D.F. (Mexico); Mendoza, D. [National Institute of Nuclear Research.. Carr. Mexico-Toluca Km 36.5 (52045), Salazar, Ocoyoacac, Edo. de Mexico (Mexico); Juarez, F. [Institute of Geophysics, National University of Mexico, Circuito Institutos, CU (04510) Mexico, D.F. (Mexico); Cabrera, L. [Faculty of Chemistry, National University of Mexico, Building D, CU (O4510) Mexico, D.F. (Mexico)

    2005-12-01

    A cave (102 m long) under the structure of the Sun pyramid of the prehispanic Teotihuacan City indicates the importance of the pyramid. Studies of the cave mortar samples using energy dispersive X-ray (EDX) fluorescence, scanning electron microscopy (SEM) and X-ray diffraction (XRD) showed no difference in the chemical elemental composition. The elements can be distributed in three groups: major, minor and trace elements. The minerals identified were compatible with the origins of the cave and with the magnetic pattern.

  1. Exposure to radon in caves and abandoned mines

    International Nuclear Information System (INIS)

    Dixon, D.W.

    1996-01-01

    The significance for health of exposure to radon daughters during leisure excursions in discussed mines or caves is considered for visitors with interests in speleology, mineral collecting, mining history and youth training. General members of the public might also enter tunnels. Potential annual exposures based on radon measurements in various mines and caves are estimated and it is concluded that the annual exposure of individuals who undertake frequent and prolonged trips to underground systems might exceed 10 6 Bq h m -3 . Exposures to general members of the public are likely to be much lower. The National Radiological Protection Board has developed proposals for a coherent and comprehensive scheme to promote dose limitation in a wide range of circumstances. These are described, with current initiatives on consultation with National Associations, local Government and many special interest groups. (author)

  2. Bermuda: Search for Deep Water Caves 2009 on the R/V Endurance between 20090905 and 20090930

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Deep-water marine caves are one of the Earth's last largely unexplored frontiers of undiscovered fauna (animal life). More than 150 limestone caves are known to...

  3. Seasonal variations of radon and the radiation exposure levels in Nerja cave, Spain

    International Nuclear Information System (INIS)

    Duenas, C.; Fernandez, M.C.; Canete, S.; Perez, M.; Gordo, E.

    2011-01-01

    222 Rn concentrations in the air in Nerja cave (Spain) (3 o 52'35''W 36 o 43'50''N) were measured by continuous monitoring using Alpha-Guard, Genitron instrument equipment. The 222 Rn measurements were carried out for a complete annual cycle in the different halls: Vestibule hall from July 2003 to June 2004, Ballet hall from July 2004 to June 2005 and Mirador hall from July 2005 to June 2006. Starting from the entrance of the cave we successively find the Vestibule hall, the Ballet hall and the Mirador hall. The range of 222 Rn levels were of 8-627 Bq m -3 for the Vestibule hall, 28-575 Bq m -3 for the Ballet hall and 38-578 Bq m -3 for the Mirador. The aim of this study was to detect seasonal variation patterns of 222 Rn concentrations. The seasonal variations of 222 Rn concentrations are discussed in relation to various meteorological factors measured inside and outside the cave. The radiation exposure levels for workers and tourists with different equilibrium factors have been evaluated. The radiation exposure levels for workers and tourists only represent a low percentage of the exposure guides for the general population. - Highlights: → The aim of the study was to detect seasonal variation of 222 Rn concentrations. → 222 Rn concentrations in the air-cave were measured by continuous monitoring. → The 222 Rn measurements were carried out in the different halls of the cave. → The radiation exposure levels for workers and tourists have been evaluated.

  4. Earthquakes as collapse precursors at the Han-sur-Lesse Cave in the Belgian Ardennes

    Science.gov (United States)

    Camelbeeck, Thierry; Quinif, Yves; Verheyden, Sophie; Vanneste, Kris; Knuts, Elisabeth

    2018-05-01

    Collapse activation is an ongoing process in the evolution of karstic networks related to the weakening of cave vaults. Because collapses are infrequent, few have been directly observed, making it challenging to evaluate the role of external processes in their initiation and triggering. Here, we study the two most recent collapses in the Dôme chamber of the Han-sur-Lesse Cave (Belgian Ardenne) that occurred on or shortly after 3rd December 1828 and between the 13th and 14th of March 1984. Because of the low probability that the two earthquakes that generated the strongest ground motions in Han-sur-Lesse since 1800, on 23rd February 1828 (Mw = 5.1 in Central Belgium) and 8th November 1983 (Mw = 4.8 in Liège) occurred by coincidence less than one year before these collapses, we suggest that the collapses are related to these earthquakes. We argue that the earthquakes accelerated the cave vault instability, leading to the collapses by the action of other factors weakening the host rock. In particular, the 1828 collapse was likely triggered by a smaller Mw = 4.2 nearby earthquake. The 1984 collapse followed two months of heavy rainfall that would have increased water infiltration and pressure in the rock mass favoring destabilization of the cave ceiling. Lamina counting of a stalagmite growing on the 1828 debris dates the collapse at 1826 ± 9 CE, demonstrating the possibility of dating previous collapses with a few years of uncertainty. Furthermore, our study opens new perspectives for studying collapses and their chronology both in the Han-sur-Lesse Cave and in other karstic networks. We suggest that earthquake activity could play a stronger role than previously thought in initiating cave collapses.

  5. Hearth-side socioeconomics, hunting and paleoecology during the late Lower Paleolithic at Qesem Cave, Israel.

    Science.gov (United States)

    Stiner, Mary C; Gopher, Avi; Barkai, Ran

    2011-02-01

    The late Lower Paleolithic archaeofaunas of Qesem Cave in the southern Levant span 400-200 ka and associate with Acheulo-Yabrudian (mainly Amudian) industries. The large mammals are exclusively Eurasian in origin and formed under relatively cool, moist conditions. The zooarchaeological findings testify to large game hunting, hearth-centered carcass processing and meat sharing during the late Lower Paleolithic, not unlike the patterns known from Middle and Upper Paleolithic caves in the region. Well-defined hearth features are rarely preserved in Qesem Cave, but the heterogeneous distributions of burned bones indicate areas of frequent hearth rebuilding throughout the occupation sequence. The hominins delayed consumption of high quality body parts until they could be moved to the cave, where hearths were hubs of processing activities and social interaction. Paradoxically, the cut marks on the Qesem bones are both more abundant and more randomly oriented than those observed in Middle and Upper Paleolithic cases in the Levant. These results suggest that several individuals were directly involved in cutting meat from the bones and that the social mechanics of meat sharing during the late Lower Paleolithic at Qesem Cave differed from those typical of both the Middle and Upper Paleolithic in the region. Copyright © 2010 Elsevier Ltd. All rights reserved.

  6. Sexual characteristics of male guppies Poecilia reticulata serve as effect biomarkers of estrogens

    Science.gov (United States)

    Tian, Hua; Li, Yun; Wang, Wei; Zhao, Fei; Gao, Su; Ru, Shaoguo

    2017-10-01

    Guppies (Poecilia reticulata) are considered a candidate model species for the identification and testing of endocrine-disrupting chemicals. Male guppies may be used to address the challenge of making potential linkages between alterations of biomarkers, both at the cellular and organ level, and adverse outcomes. In the present study, a predictive relationship between sex characteristics and reproductive output was observed in male guppies that underwent a long-term toxicity test with 0.5 μg/L 17β-estradiol administered during the juvenile period. Radioimmunoassay and western blot analyses demonstrated that 17β-estradiol exposure caused a significant increase in testicular 17β-estradiol levels as well as the induction of exposure biomarkers, namely hepatic vitellogenin. Exposure to 17β-estradiol also caused a significant decrease in testosterone levels, which consequently reduced the gonadosomatic index, sperm counts, and the coloration index. These changes of male sexual characteristics further translated into adverse influences on reproduction, as measured by a decrease in off spring production and survival rate. Our results suggest that the above-mentioned sexual characteristics of male guppies may be considered potential in vivo biomarkers of estrogen effects on reproduction.

  7. IDENTIFICATION AND BIOLOGICAL ACTIVITY OF POTENTIAL PROBIOTIC BACTERIA ISOLATED FROM POECILIA RETICULATA (GUPPY

    Directory of Open Access Journals (Sweden)

    Aparna Balakrishna

    2014-08-01

    Full Text Available Antagonistic activities against candidate indicator strains, adhesion to mucus and biofilm formation of potential probiotic strains isolated from Poecilia reticulata were evaluated. Four isolated strains (MBTU_PB1, MBTU_PB2, MBTU_PB3 and MBTU_PB4 showed moderate to strong antagonistic activities against the tested five indicator strains (Aeromonas hydrophila1739, Vibrio cholera 3906, Flavobacterium 2495, Acinetobacter 1271 and Alcaligenes 1424 and these isolates were further identified using biochemical tests and 16S rDNA gene sequence analysis. Except the whole cell product, the other three cellular components, namely, heat-killed whole cell product, intracellular product and extracellular product of all the four selected isolates were equally effective, as revealed by the zone of inhibitions to the tested indicator strains. The in vitro adhesion property or the ability of colonization is often considered as a selection criteria for probiotics. All the selected four strains had higher adhesion abilities than the indicator strains. Further, these four strains had the ability to form biofilms on polystyrene surfaces. The in vitro characterization of these four strains suggests possibility of using the isolates, as individual strain or in combination, for probiotic therapy in aquaculture.

  8. Nutritional Evaluation of Raw Materials Entering the Structure to Mixed Fodder for the Specie Poecilia reticulata (Guppy

    Directory of Open Access Journals (Sweden)

    Adrian Gruber

    2011-05-01

    Full Text Available In the ornamental pisciculture is a especial emphasis on the exterior shape and color to the fishes, issues that are dependent directly to the structure of compound feeds in relation to the nutritional characteristics of the raw materials.Own research or focused on analyzing the crude chemical composition with Weende scheme (water content and dry matter, crude ash, crude protein, crude fat, crude fiber, SEN of raw materials can be used in the structure of a compound feeds for the Poecilia reticulata (guppy species, for most of these materials there are no current data in the literature.These materials were analyzed: gelatin, wheat flour, sunflower meal, soybean meal, meal Spirulina platensis, carrot (Daucus carota, Pangasius fillet, Daphnia pulex, grount dandelion (Taraxacum officinale, grount nettle (Urtica dioica and yeast.

  9. Analysis of the main factors affecting the evaluation of the radon dose in workplaces: The case of tourist caves

    International Nuclear Information System (INIS)

    Sainz, Carlos; Quindos, Luis Santiago; Fuente, Ismael; Nicolas, Jorge; Quindos, Luis

    2007-01-01

    High concentrations of radon exist in several workplaces like tourist caves mainly because of the low ventilation rates existing at these enclosures. In this sense, in its 1990 publication, the ICRP recommended that high exposures of radon in workplaces should be considered as occupational exposure. In developed caves in which guides provide tours for the general public great care is needed for taking remedial actions concerning radon, because in some circumstances forced ventilation may alter the humidity inside the cave affecting some of the formations or paintings that attract tourists. Tourist guides can work about 1900 h per year, so the only option to protect them and other cave workers from radon exposure is to apply an appropriate system of radiation protection mainly based on limitation of exposure by restricting the amount of time spent in the cave. Because of the typical environmental conditions inside the caves, the application of these protecting actions requires to know some indoor air characteristics like particle concentration, as well as radon progeny behaviour in order to get more realistic effective dose values In this work the results of the first two set of radon measurements program carried out in 10 caves located in the region of Cantabria (Spain) are presented

  10. Unusual dominance by desert pupfish (Cyprinodon macularius) in experimental ponds within the Salton Sea Basin

    Science.gov (United States)

    Saiki, Michael K.; Martin, Barbara A.; Anderson, Thomas W.

    2011-01-01

    In October 2006, months after shallow experimental ponds in the Salton Sea Basin were filled with water from the Alamo River and Salton Sea, fish were observed in several ponds, although inlets had been screened to exclude fish. During October 2007November 2009, nine surveys were conducted using baited minnow traps to document species and relative abundance of fish. Surveys yielded 3,620 fish representing five species. Desert pupfish (Cyprinodon macularius), the only native species encountered, was the most numerous and comprised >93% of the catch. Nonnative species included western mosquitofish (Gambusia affinis, 4.1%), sailfin molly (Poecilia latipinna, 2.8%), and tilapia (a mixture of hybrid Mozambique tilapia Oreochromis mossambicus ?? O. urolepis and redbelly tilapia Tilapia zillii, <0.1%). Dominance by desert pupfish, which persisted over our 2 years of study, was unusual because surveys conducted in nearby agricultural drains yielded relatively few desert pupfish.

  11. Aerosol particle size distribution in building and caves: impact to the radon-related dose evaluation

    International Nuclear Information System (INIS)

    Berka, Z.; Thinova, L.; Brandejsova, E.; Zdimal, V.; Fronka, A.; Milka, D.

    2004-01-01

    The results of evaluation of the aerosol particle size spectra observed in the Bozkov cave are presented and compared with the spectra observed in residential areas. The radon-to-dose conversion factor is discussed, as is the correction factor referred to as the cave factor. (P.A.)

  12. Distribution of meiofaunal abundances in a marine cave complex with secondary openings and freshwater filtrations

    DEFF Research Database (Denmark)

    Riera, Rodrigo; Monterroso, Óscar; Núñez, Jorge

    2018-01-01

    Submerged sea caves are priority areas for conservation according to the Habitat Directive 92/43/CEE because of their unique biodiversity. A limited number of publications exist about communities living on sediments inside caves, mostly focused on the macrofaunal fraction (>0.5-mm body size). Mei...

  13. Collembola of Rapa Nui (Easter Island) with descriptions of five endemic cave-restricted species.

    Science.gov (United States)

    Bernard, Ernest C; Soto-Adames, Felipe N; Wynne, J Judson

    2015-04-24

    Eight species of Collembola are reported from recent collections made in caves on the Polynesian island of Rapa Nui (Easter Island). Five of these species are new to science and apparently endemic to the island: Coecobrya aitorererere n. sp., Cyphoderus manuneru n. sp., Entomobrya manuhoko n. sp., Pseudosinella hahoteana n. sp. and Seira manukio n. sp. The Hawaiian species Lepidocyrtus olena Christiansen & Bellinger and the cosmopolitan species Folsomia candida Willem also were collected from one or more caves. Coecobrya kennethi Jordana & Baquero, recently described from Rapa Nui and identified as endemic, was collected in sympatric association with C. aitorererere n.sp. With the exception of F. candida, all species are endemic to Rapa Nui or greater Polynesia and appear to be restricted to the cave environment on Rapa Nui. A key is provided to separate Collembola species reported from Rapa Nui. We provide recommendations to aid in the conservation and management of these new Collembola, as well as the other presumed cave-restricted arthropods.

  14. New updated results of paleomagnetic dating of cave deposits exposed in Za Hájovnou Cave, Javoříčko Karst

    Czech Academy of Sciences Publication Activity Database

    Kadlec, Jaroslav; Čížková, Kristýna; Šlechta, Stanislav

    2014-01-01

    Roč. 70, 1-2 (2014), s. 27-34 ISSN 0036-5343 Institutional support: RVO:67985530 ; RVO:67985831 Keywords : Javoříčko Karst * Za Hájovnou Cave * Early and Middle Pleistocene * paleomagnetic dating Subject RIV: DB - Geology ; Mineralogy

  15. iCAVE: an open source tool for visualizing biomolecular networks in 3D, stereoscopic 3D and immersive 3D.

    Science.gov (United States)

    Liluashvili, Vaja; Kalayci, Selim; Fluder, Eugene; Wilson, Manda; Gabow, Aaron; Gümüs, Zeynep H

    2017-08-01

    Visualizations of biomolecular networks assist in systems-level data exploration in many cellular processes. Data generated from high-throughput experiments increasingly inform these networks, yet current tools do not adequately scale with concomitant increase in their size and complexity. We present an open source software platform, interactome-CAVE (iCAVE), for visualizing large and complex biomolecular interaction networks in 3D. Users can explore networks (i) in 3D using a desktop, (ii) in stereoscopic 3D using 3D-vision glasses and a desktop, or (iii) in immersive 3D within a CAVE environment. iCAVE introduces 3D extensions of known 2D network layout, clustering, and edge-bundling algorithms, as well as new 3D network layout algorithms. Furthermore, users can simultaneously query several built-in databases within iCAVE for network generation or visualize their own networks (e.g., disease, drug, protein, metabolite). iCAVE has modular structure that allows rapid development by addition of algorithms, datasets, or features without affecting other parts of the code. Overall, iCAVE is the first freely available open source tool that enables 3D (optionally stereoscopic or immersive) visualizations of complex, dense, or multi-layered biomolecular networks. While primarily designed for researchers utilizing biomolecular networks, iCAVE can assist researchers in any field. © The Authors 2017. Published by Oxford University Press.

  16. CONTROL DEMONSTRATION OF THE RICEFIELD BREEDING MOSQUITO ANOPHELES ACONITUS DONITZ IN CENTRAL JAVA, USING POECILIA RETICULATA THROUGH COMMUNITY PARTICIPATION : 3. FIELD TRIAL AND EVALUATION

    Directory of Open Access Journals (Sweden)

    Sustriayu Nalim

    2012-09-01

    Full Text Available Suatu penelitian dilakukan untuk menjajagi kemungkinan menggunakan minapadi sebagai cara pemberantasan nyamuk Anopheles aconitus. Minapadi dilakukan dengan ikan Cyprinus carpio. Dengan tersedianya air di sawah untuk ikan, ikan pemakan jentik Poecilia Reticulata yang ditebarkan dapat hidup pula. Penebaran dilakukan oleh masyarakat. Hasil Penelitian ini menunjukkan bahwa melalui minapadi yaitu dengan penebaran, ikan P. reticulata pada kepadatan 2 ekor /m2, populasi An. aconitus selama 5 tahun dapat diturunkan sebanyak 99,7% dan S.P.R. malaria sebanyak 98.8%. [1] Regency Health Service, Banjarnegara Regency, Central Java.

  17. Cave development in an uplifting fold-and-thrust belt of the Tatra Mountains, Poland

    Directory of Open Access Journals (Sweden)

    Jacek Szczygiel

    2015-09-01

    Full Text Available Detailed structural analysis and geomorphological observations supplemented by the analysis of the distribution of karst conduit directions have been performed in 23 morphologically diverse caves in the Tatra Mountains. Based on these studies, a development scheme of vadose cave passages has been proposed for the most common geological settings in the fold-and-thrust-belt: (1 single-plain faults, (2 multiple fault cores, (3 bedding plane fractures and (4 hinge zones of recumbent folds. Results indicate that the dynamics of the massif (local gravity sliding in the nearby slope zone and regional stress fields, along with the structural pattern, influences the predisposition of structural and stratigraphic discontinuities to karst drainage. Constant tectonic stress fields affected the massif during the entire speleogenesis. This led to the rejuvenation of the same displacements in successive tectonic events, which resulted in promoting this reactivated structures in successive speleogenetic phases. Structures along which older cave levels had developed were also utilized later by vadose and phreatic drainage, leading to the intersection of the vadose passages with elevated paleo-phreatic cave levels. Independently, formation of entirely vadose caves, guided by the same group of weak and rejuvenated planes, was enabled. In the Tatras, the concentric and recumbent geometry of the main folds resulted in steep dipping of the bedding planes over a distance up to a few hundred meters which makes the bedding plane fractures subject to karst water circulation in these geologic and geodynamic settings.

  18. A clustering approach applied to time-lapse ERT interpretation - Case study of Lascaux cave

    Science.gov (United States)

    Xu, Shan; Sirieix, Colette; Riss, Joëlle; Malaurent, Philippe

    2017-09-01

    The Lascaux cave, located in southwest France, is one of the most important prehistoric cave in the world that shows Paleolithic paintings. This study aims to characterize the structure of the weathered epikarst setting located above the cave using Time-Lapse Electrical Resistivity Tomography (ERT) combined with local hydrogeological and climatic environmental data. Twenty ERT profiles were carried out for two years and helped us to record the seasonal and spatial variations of the electrical resistivity of the hydraulic upstream area of the Lascaux cave. The 20 interpreted resistivity models were merged into a single synthetic model using a multidimensional statistical method (Hierarchical Agglomerative Clustering). The individual blocks from the synthetic model associated with a similar resistivity variability were gathered into 7 clusters. We combined the resistivity temporal variations with climatic and hydrogeological data to propose a geo-electrical model that relates to a conceptual geological model. We provide a geological interpretation for each cluster regarding epikarst features. The superficial clusters (no 1 & 2) are linked to effective rainfall and trees, probably a fractured limestone. Another two clusters (no 6 & 7) are linked to detrital formations (sand and clay respectively). The cluster 3 may correspond to a marly limestone that forms a non-permeable horizon. Finally, the electrical behavior of the last two clusters (no 4 & 5) is correlated with the variation of flow rate; they may be a privileged feed zone of the flow in the cave.

  19. Assessing the origin of unusual organic formations in lava caves from Canary Islands (Spain)

    Science.gov (United States)

    Miller, Ana Z.; de la Rosa, Jose M.; Garcia-Sanchez, Angela M.; Pereira, Manuel F. C.; Jurado, Valme; Fernández, Octavio; Knicker, Heike; Saiz-Jimenez, Cesareo

    2016-04-01

    Lava tubes, like other caves, contain a variety of speleothems formed in the initial stage of a lava tube formation or due to leaching and subsequent precipitation of secondary minerals. Primary and secondary mineral formations in lava caves are mainly composed of silicate minerals, although secondary minerals common in limestone caves have been also reported in this type of caves. In addition, unusual colored deposits have been found on the walls and ceilings of lava tubes, some of them of unknown origin and composition. A brown to black-colored mud-like deposits was observed in "Llano de los Caños" Cave, La Palma Island, Canary Islands, Spain. These black deposits coat the wall and ceiling of the lava tube where sub-horizontal fractures occur. FESEM-EDS, X-ray micro-computed tomography and mineralogical analyses were conducted for morphological, 3D microstructural and compositional characterization of these unusual speleothem samples. These techniques revealed that they are mainly composed of amorphous materials, suggesting an organic carbon composition. Hence, analytical pyrolysis (Py-GC/MS), solid-state 13C Nuclear Magnetic Resonance (NMR) and stable isotope analysis were applied to assess the nature and origin of the black deposits. The combination of these analytical tools permits the identification of specific biomarkers (di- and triterpenoids) for tracing the potential sources of the organic compounds in the speleothems. For comparison purposes, samples from the topsoil and overlaying vegetation were also analyzed. Chromatograms resulting from the Py-GC/MS showed an abundance of polysaccharides, lipids and terpenoids typically derived from the vegetation of the area (Erica arborea). In addition, levoglucosan, polycyclic aromatic hydrocarbons and N-containing heterocyclic compounds were detected. They probably derived from the leaching of charred vegetation resulting from a wildfire occurred in the area in 2012. The lack of the typical pattern of odd

  20. The Snezhnaya-Mezhennogo-Illyuziya cave system in the western Caucasus; El sistema de cuevas Snezhnaya-Mezhennogo-Illyuziya en el Caucaso occidental

    Energy Technology Data Exchange (ETDEWEB)

    Mavlyudov, B. R.

    2016-07-01

    The Snezhnaya-Mezhennogo-Illyuziya cave system (SMI) is located within the Khipstinsky karstic massif, in the Western Caucasus. The cave is a branched, arborescent system of cave channels through which underground water streams flow and change in an upwards direction in sub-vertical shafts. Now 3 such shafts, which have a connection with the cave river, are being studied: the Snezhnaya (1970 m a.s.l.), the Mezhennogo (2 015 m a.s.l.) and the Illyuziya (2 389 m a.s.l.). The SMI cave system has been investigated since 1971 and the currently known depth of the system is 1 760 m, the extent of the galleries ≥32 km, the volume ≥2.7 million m3, the specific volume - 84 m{sup 3}/m. The size of the biggest cave chamber the Thronnyj - is 309x109x40 m. The average discharge of the underground river is about 500 l/s. The temperature in the cavity changes from 0 to 6.5 degree centigrade. Research on the SMI cave system continues. (Author)

  1. Luminescence dating at Rose cottage cave: a progress report

    CSIR Research Space (South Africa)

    Woodborne, S

    1997-10-01

    Full Text Available Deal with infrared-stimulated luminescence and thermoluminescence dates from Rose Cottage Cave in South Africa. Discrepancy between luminescence and radiocarbon dates; Concentration of radioactive elements in sediments before and after leaching...

  2. Sediments of Biśnik Cave (Poland): Lithology and stratigraphy of the Middle Palaeolithic site

    Czech Academy of Sciences Publication Activity Database

    Krajcarz, M. T.; Bosák, Pavel; Šlechta, Stanislav; Pruner, Petr; Komar, M.; Dresler, J.; Madeyska, T.

    326/327, April (2014), s. 6-19 ISSN 1040-6182 R&D Projects: GA AV ČR IAA300130701 Institutional research plan: CEZ:AV0Z30130516 Institutional support: RVO:67985831 Keywords : karsology * caves * Biśnik Cave (Poland) Subject RIV: DB - Geology ; Mineralogy Impact factor: 2.062, year: 2014

  3. Longwall top coal caving (LTCC) mining technologies with roof softening by hydraulic fracturing method

    Science.gov (United States)

    Klishin, V.; Nikitenko, S.; Opruk, G.

    2018-05-01

    The paper discusses advanced top coal caving technologies for thick coal seams and addresses some issues of incomplete coal extraction, which can result in the environmental damage, landscape change, air and water pollution and endogenous fires. The authors put forward a fundamentally new, having no equivalent and ecology-friendly method to difficult-to-cave roof coal – directional hydraulic fracturing and nonexplosive disintegration.

  4. On some new cave-dwelling ground beetles (Coleoptera: Carabidae: Trechini from eastern Serbia

    Directory of Open Access Journals (Sweden)

    Vrbica Maja

    2013-01-01

    Full Text Available The following new cavernicolous ground beetle taxa are described from three caves in eastern Serbia: Duvalius (Paraduvalius trifunovici sp. n., from the Mandina Pećina Cave, village of Zlot, near Bor, Kučajske Planine Mts., D. (P. rtanjensis sp. n., from the Golema Porica Pit, Mt. Rtanj, and Glabroduvalius gen. n., G. tupiznicensis sp. n., from the Gornja Lenovačka Pećina Cave, village of Lenovac, near Zaječar, Mt. Tupižnica. The new taxa are easily distinguished from related organisms. All important morphological features have been listed, along with the diagnoses and illustrations of the taxa. The new taxa are relicts and endemics of eastern Serbia and probably belong to old phyletic lineages of Tertiary or even pre-Tertiary origin. [Projekat Ministarstva nauke Republike Srbije, br. 173038, br. 43001 i br. 43002

  5. Estrutura populacional de Poecilia vivípara Bloch & Schneider, 1801 (Atheriniformes, Poeciliidae do rio Ceará-Mirim - Rio Grande do Norte Populational structure of Poecilia vivipara Block & Schneider, 1801 (Atheriniformes, Poeciliidae of Ceará-Mirim river, State of Rio Grande do Norte

    Directory of Open Access Journals (Sweden)

    Renata Swany Soares Nascimento

    2000-05-01

    Full Text Available Com o propósito de elucidar aspectos da estrutura populacional de Poecilia vivipara do rio Ceará-Mirim, município de Poço Branco, Rio Grande do Norte, foram capturados 3.390 exemplares em coletas mensais, no período de junho de 1995 a maio de 1997, utilizando-se tarrafas e peneiras. A partir dos resultados obtidos, constatou-se que as fêmeas, em relação aos machos, predominaram na proporção de 8:1, considerando-se as análises total e sazonal. No que se refere à distribuição por classes de comprimento para os sexos separados, observou-se uma maior amplitude de comprimento das fêmeas em relação aos machos e ainda uma ausência de diferenças entre as estações chuvosa e seca. De acordo com os valores obtidos para o &Teta; (3,86, verificou-se, para a espécie, um crescimento do tipo alométrico positivo. Quanto à influência dos fatores abióticos, fotoperíodo, pluviosidade, temperatura e nível do rio, relacionados aos aspectos da estrutura populacional, não foram verificados resultados significativos que a confirmassemWith the aim of clarifying aspects of the populational structure of Poecilia vivipara in the Ceará-Mirim river, State of Rio Grande do Norte, a total of 3,390 specimens were captured in monthly collections June 1995 trough May 1997, using nets and sieves. Results showed that females, prevailed in the proportion of 8:1, in the whole period as well as seasonaly. The total length classes distribution for separate sexes indicated that females reaches greatest lengths than males with no difference between dry and rainy seasons. The species growth is of the alometric positive type as suggested by the &Teta; value (3,86. No correlation between abiotic factors (photoperiod duration, rainfall, temperature and river water level and the population structure was found

  6. Speleothems from Mawsmai and Krem Phyllut caves, Meghalaya, India: some evidences on biogenic activities

    Science.gov (United States)

    Baskar, Sushmitha; Baskar, R.; Lee, Natuschka; Theophilus, P. K.

    2009-05-01

    The Mawsmai cave and Krem Phyllut caves, East Khasi hills, Meghalaya, India has so far not yet attracted the attention of geomicrobiologists. Observations and hypotheses on the possible influence of identified microorganisms for speleothem formations in Meghalaya are reported for the first time. XRD studies identified calcite in speleothems and gypsum in cave wall deposits as the dominant minerals. SEM-EDAX showed interesting microfabric features showing strong resemblance with fossilised bacteria, calcified filaments, needle calcite and numerous nano scale calcite crystals, highly weathered and disintegrated crystals of calcite, that point towards a significant microbial influence in its genesis. Thin section petrography showed laminated stromatolitic features. The microorganisms identified by conventional isolation and further evaluation of isolates by molecular techniques include Bacillus cereus, Bacillus mycoides, Bacillus licheniformis, Micrococcus luteus, and Actinomycetes. Microscopic observations also showed unidentifiable cocci and four unidentifiable strains of CaSO4 (gypsum) precipitating bacteria. Experimental studies confirmed that these bacteria are able to precipitate calcium minerals (calcite, gypsum, minor amounts of dolomite) in the laboratory. These results allow us to postulate that species like these may contribute to active biogenic influence in the cave formations at Meghalaya.

  7. The Vie Cave Geomorphological Site in Southern Tuscany (Italy: Problems of Decay and Conservation

    Directory of Open Access Journals (Sweden)

    Elena Pecchioni

    2015-06-01

    Full Text Available The Vie Cave are a suggestive network of roads deeply entrenched in the rock, dating back to the Etruscan civilization; these ancient roads connect various settlements and necropolises existing mainly in the area of Sovana, Sorano and Pitigliano towns (Southern Tuscany, Italy. The Vie Cave are located in a peculiar geomorphological site, characterized by the presence of extensive pyroclastic deposits, which have been incised by a parallel network of deep gorges. In this paper, the geomorphological, geological and lithological setting of the Vie Cave area, where several Etruscan archaeological sites are found, are described. The precarious stability of the Vie Cave walls and the several archaeological structures carved into them, the high grade of decay shown by the constituent materials, together with the dense vegetation that has developed over the rocky scarps, are taken into account with the aim to provide a complete assessment of the conditions in which the site lies. Finally, we propose some targeted actions related to the preservation of this territory, showing so distinctive morphology, in order to protect the area from further decay to which it would be subjected if it remained abandoned.

  8. Low regional diversity of late cave bears mitochondrial DNA at the time of Chauvet Aurignacian paintings

    NARCIS (Netherlands)

    Bon, Celine; Berthonaud, Veronique; Fosse, Philippe; Gely, Bernard; Maksud, Frederic; Vitalis, Renaud; Philippe, Michel; van der Plicht, Johannes; Elalouf, Jean-Marc

    The Chauvet-Pont d'Arc and Deux-Ouvertures caves, located along the Ardeche River (France), contain abundant remains of the extinct cave bear (Ursus spelaeus). Because they also display a variety of Palaeolithic anthropogenic evidences, such as the earliest charcoal drawings recorded to date

  9. The bat fauna of the Kararaô and Kararaô Novo caves in the area under the influence of the Belo Monte hydroelectric dam, in Pará, Brazil.

    Science.gov (United States)

    Zortéa, M; Bastos, N A; Acioli, T C

    2015-08-01

    Brazil's large territory displays significant richness in caves with about 12 thousand caves already recorded. Nevertheless, studies on bats in these environments are extremely scarce and fragmented. This study characterized the chiropteran fauna from two sandstone caves under the influence of the Belo Monte hydroelectric dam (Belo Monte UHE) in Pará, Brazil. The Kararaô and Kararaô Novo caves are located on the same ridge, 250 m apart. Three expeditions were carried out in 2013 and 2014, with a 4- to 5-month interval in between. A total of 589 animals were caught, 246 in the Kararaô cave and 343 in the Kararaô Novo cave. Fifteen species were recorded (13 in each cave) representing 79% similarity. With the exception of Vampyrum spectrum, which is not a cave species, the remaining recorded species were mostly cave bat species. Some species seemed to use the caves seasonally, although the basis of this pattern is still unknown. The most commonly observed species were Pteronotus personatus (dominant in the Kararaô cave), P. parnellii (dominant in the Kararaô Novo cave), and Lionycteris spurrelli, which accounted for 65% of all captures recorded for the two caves. Natalus macrourus is a species recorded in the Kararaô cave that is regionally threatened with extinction. Both caves are less than 500 m from the future reservoir; however, because the Kararaô cave entry is in an area that is lower than the reservoir, it can suffer alterations that would affect its dynamics. This raises great concern about the cave's associated fauna.

  10. Anthropogenic infilling of a Bermudian sinkhole and its impact on sedimentation and benthic foraminifera in the adjacent anchialine cave environment

    Directory of Open Access Journals (Sweden)

    Jacquelyn N. Cresswell

    2017-09-01

    Full Text Available In the mid-20th century, an inland brackish pond from Bermuda, known as Eve’s Pond, was filled with marine sediment from an adjacent coastal lagoon. At this time, an eyewitness reported “…sediment billowing out of the Green Bay Cave for days…”, which is a marine-dominated anchialine cave located proximal to the former location of Eve’s Pond (~200 m. The purpose of this study was to evaluate the potential impact of this infilling event on cave sedimentation and benthic meiofaunal communities, as proxied by the unicellular protists foraminifera that remain preserved in the sediment record. Eight sediment cores were collected from an underwater passage in Green Bay Cave in a transect towards the location where Eve’s Pond was surveyed in 1901 CE. The sediment cores were analyzed for visual and density changes (photography, X-radiography, textural variability, benthic foraminifera fauna and diversity, and radiocarbon dating. The recovered sediment cores mostly sampled a late Holocene carbonate mud facies that had been described during previous research in the cave, with benthic foraminiferal assemblages post-dating the onset of seawater circulating between the saline groundwater flooding the cave and the adjacent Harrington Sound ~1,900 years ago. However, two cores located further into the cave (cores 13 and 17 contain a carbonate sand layer with lagoon foraminifera that is anomalous with respect to the Holocene depositional history of the cave and is most likely related to the mid-20th century infilling of Eve’s Pond. Examination of these two cores showed that after the infilling event, the community of benthic foraminifera rapidly reverted to pre-impact assemblages with foraminiferal stygophiles (e.g., Spirophthalmidium emaciatum, Sigmoilina tenuis, which were not displaced by new colonizers introduced into the cave by the dredge spoils. We caution that the results cannot be extrapolated to the pelagic crustacean community, but the

  11. Integrated geophysical surveys to assess the structural conditions of a karstic cave of archaeological importance

    Directory of Open Access Journals (Sweden)

    G. Leucci

    2005-01-01

    Full Text Available An integrated geophysical survey using both the electrical resistivity tomography (ERT and ground-penetrating radar (GPR methods was undertaken over a cave of great archaeological interest in southern Italy. The survey was performed to assess the stability of the carbonate rock roof of the cave. A geophysical survey was preferred to boreholes and geotechnical tests, in order to avoid the risk of mass movements. The interpretation of integrated data from ERT and GPR resulted in an evaluation of some of the electromagnetic (EM characteristics (such as the EM wave velocity and the detection of discontinuities (fractures in the carbonate rock. It is well known that rock fractures constitute a serious problem in cave maintenance, and progressive cracking within the bed rock is considered to be one of the main causes of collapse. An analysis of the back-scattered energy was also required for the GPR data interpretation. Cracks within the bedrock were detected to a depth of about 2 m by using GPR, which allowed for the identification of the loosened zone around the cave.

  12. Do benthic sediment characteristics explain the distribution of juveniles of the deposit-feeding sea cucumber Australostichopus mollis?

    Science.gov (United States)

    Slater, Matthew J.; Jeffs, Andrew G.

    2010-10-01

    Despite the economic importance of many deposit-feeding sea cucumbers, the ecology of their juveniles is poorly understood and factors influencing juvenile habitat selection remain largely unexplained. We investigated the importance of the characteristics of the available sediment in determining the highly localised distribution of juveniles of the deposit-feeding Australasian sea cucumber Australostichopus mollis. Wild-caught juveniles were displaced to non-juvenile habitats with surface sediments characterised by lower total organic content (TOM) and nitrogen content, higher chlorophyll- a content and coarser grain size profiles compared to juvenile sites. The growth of displaced individual animals was monitored over 9 months and compared to control animals caged in the juvenile habitats. Displaced juvenile sea cucumbers had high survival rates that did not differ significantly from juvenile habitats. Displaced juveniles exhibited significantly higher specific growth rate (SGR) than those at juvenile sites ( p < 0.001), although the growth of individuals was highly variable within individual cages and among sites. The lower TOM and nitrogen content, and coarser grain size profiles at non-juvenile sites did not result in reductions in juvenile survival or growth. Higher microphytobenthic activity may have resulted in the higher growth rates observed at shallow non-juvenile sites. The SGR of juveniles over the first 6 months of the experiment ranged between 0.45% d - 1 and 0.74% d - 1 for all sites. This was followed by marked growth limitation between 6 and 9 months either as a result of increasing juvenile biomass in cages or seasonal growth limitation. A subsequent reduction in juvenile density resulted in markedly increased growth over the following 3 month period. Juvenile A. mollis show an ability to exploit a variety of benthic sediment food sources, indicating that their highly localised distribution is not due to differences in the food quality of

  13. DNA analysis of fecal bacteria to augment an epikarst dye trace study at Crump's Cave, Kentucky

    Science.gov (United States)

    A rainfall simulation experiment was performed to investigate the transport behavior of fecal-derived bacteria through shallow karst soils and through the epikarst. The experiment was conducted at Cave Springs Cavern located just south of Mammoth Cave National Park on the Sinkhole Plain of South Cen...

  14. Effects of Active Exploration and Passive Observation on Spatial Learning in a CAVE

    OpenAIRE

    Melanson, Brian; Kelso, John; Bowman, Doug A.

    2002-01-01

    This experiment was a modification of Paul N. Wilson's 1999 study entitled "Active Exploration of a Virtual Environment Does Not Promote Orientation or Memory for Objects." It was hoped that changing the immersion level from a standard desktop monitor to a more immersive CAVE environment would change the results of this experiment. All subjects explored a three-dimensional virtual environment in a CAVE. Active subjects were given controls to choose their own path and explore th...

  15. Underground cosmic-ray measurement for morphological reconstruction of the ''Grotta Gigante'' natural cave

    International Nuclear Information System (INIS)

    Caffau, E.; Coren, F.; Giannini, G.

    1997-01-01

    Measurement of the muon flux as a function of direction inside the Grotta Gigante natural cave near Trieste (Italy) was carried out using a tracking apparatus. The measured flux, depending in a well established way on the zenith angle and the rock mass crossed by the muons, allowed the determination of the shape of the cave vault which could be compared with that known from topography and related to the available microgravimetric data of the area. (orig.)

  16. A note on the occurrence of a crayback stalagmite at Niah Caves, Borneo

    Directory of Open Access Journals (Sweden)

    Lundberg Joyce

    2011-01-01

    Full Text Available Crayback stalagmites have mainly been reported from New South Wales, Australia. Here we document a small crayback in the entrance of Painted Cave (Kain Hitam, part of the Niah Caves complex in Sarawak, Borneo. Measuring some 65 cm in length and 18 cm in height, this deposit is elongate in the direction of the dominant wind and thus oriented towards the natural tunnel entrance. It shows the classic humpbacked long profile, made up of small transverse segments or plates, in this case the tail extending towards the entrance. The dark blue-green colour down the centre suggests that cyanobacterial growth follows the track of the wind-deflected roof drip. The dry silty cave sediment provides material for accretion onto the biological mat. This is the only example known from Borneo and one of the very few known from outside of Australia.

  17. Radon continuous monitoring in Altamira Cave (northern Spain) to assess user's annual effective dose

    International Nuclear Information System (INIS)

    Lario, J.; Sanchez-Moral, S.; Canaveras, J.C.; Cuezva, S.; Soler, V.

    2005-01-01

    In this work, we present the values of radon concentration, measured by continuous monitoring during a complete annual cycle in the Polychromes Hall of Altamira Cave in order to undertake more precise calculations of annual effective dose for guides and visitors in tourist caves. The 222 Rn levels monitored inside the cave ranges from 186 Bq m -3 to 7120 Bq m -3 , with an annual average of 3562 Bq m -3 . In order to more accurately estimate effective dose we use three scenarios with different equilibrium factors (F=0.5, 0.7 and 1.0) together with different dose conversion factors proposed in the literature. Neither effective dose exceeds international recommendations. Moreover, with an automatic radon monitoring system the time remaining to reach the maximum annual dose recommended could be automatically updated

  18. An Integrated Transcriptome-Wide Analysis of Cave and Surface Dwelling Astyanax mexicanus

    Science.gov (United States)

    Gross, Joshua B.; Furterer, Allison; Carlson, Brian M.; Stahl, Bethany A.

    2013-01-01

    Numerous organisms around the globe have successfully adapted to subterranean environments. A powerful system in which to study cave adaptation is the freshwater characin fish, Astyanax mexicanus. Prior studies in this system have established a genetic basis for the evolution of numerous regressive traits, most notably vision and pigmentation reduction. However, identification of the precise genetic alterations that underlie these morphological changes has been delayed by limited genetic and genomic resources. To address this, we performed a transcriptome analysis of cave and surface dwelling Astyanax morphs using Roche/454 pyrosequencing technology. Through this approach, we obtained 576,197 Pachón cavefish-specific reads and 438,978 surface fish-specific reads. Using this dataset, we assembled transcriptomes of cave and surface fish separately, as well as an integrated transcriptome that combined 1,499,568 reads from both morphotypes. The integrated assembly was the most successful approach, yielding 22,596 high quality contiguous sequences comprising a total transcriptome length of 21,363,556 bp. Sequence identities were obtained through exhaustive blast searches, revealing an adult transcriptome represented by highly diverse Gene Ontology (GO) terms. Our dataset facilitated rapid identification of sequence polymorphisms between morphotypes. These data, along with positional information collected from the Danio rerio genome, revealed several syntenic regions between Astyanax and Danio. We demonstrated the utility of this positional information through a QTL analysis of albinism in a surface x Pachón cave F2 pedigree, using 65 polymorphic markers identified from our integrated assembly. We also adapted our dataset for an RNA-seq study, revealing many genes responsible for visual system maintenance in surface fish, whose expression was not detected in adult Pachón cavefish. Conversely, several metabolism-related genes expressed in cavefish were not detected in

  19. Center for Advanced Energy Studies: Computer Assisted Virtual Environment (CAVE)

    Data.gov (United States)

    Federal Laboratory Consortium — The laboratory contains a four-walled 3D computer assisted virtual environment - or CAVE TM — that allows scientists and engineers to literally walk into their data...

  20. Passive sampling of bioavailable organic chemicals in Perry County, Missouri cave streams.

    Science.gov (United States)

    Fox, J Tyler; Adams, Ginny; Sharum, Martin; Steelman, Karen L

    2010-12-01

    Two types of passive samplers--semipermeable membrane devices (SPMDs) and polar organic chemical integrative samplers (POCIS)--were deployed in spring 2008 to assess bioavailable concentrations of aquatic contaminants in five cave streams and resurgences in Perry County, Missouri. Study sites represent areas of high cave biodiversity and the only known habitat for grotto sculpin (Cottus carolinae). Time-weighted average (TWA) water concentrations were calculated for 20 compounds (n = 9 SPMDs; n = 11 POCIS) originating primarily from agricultural sources, including two organochlorine insecticides, dieldrin and heptachlor epoxide, which were found at levels exceeding U.S. EPA criteria for the protection of aquatic life. GIS data were used to quantify and map sinkhole distribution and density within the study area. Infiltration of storm runoff and its influence on contaminant transport were also evaluated using land cover and hydrological data. This work provides evidence of cave stream contamination by a mix of organic chemicals and demonstrates the applicability of passive samplers for monitoring water quality in dynamic karst environments where rapid transmission of storm runoff makes instantaneous water sampling difficult.

  1. Radon decay products and 10-1100 nm aerosol particles in Postojna Cave

    Science.gov (United States)

    Bezek, M.; Gregorič, A.; Vaupotič, J.

    2013-03-01

    At the lowest point along the tourist route in Postojna Cave, the activity concentration of radon (222Rn) decay products and the number concentration and size distribution of aerosol particles in the size range of 10-1100 nm were monitored, with the focus on the unattached fraction (fun) of radon decay products (RnDPs), a key parameter in radon dosimetry. The total number concentration of aerosols during visits in summer was lower (700 cm-3) than in winter (2800 cm-3), and was dominated by 50 nm particles (related to the attached RnDPs) in winter. This explains the higher fun values in summer (0.75) and the lower winter measurement (0.04) and, consequently, DCFD values of 43.6 and 13.1 mSv WLM-1 respectively for the calculated dose conversion factors. The difference is caused by an enhanced inflow of fresh outside air, driven in winter by the higher air temperature in the cave compared to outside, resulting in the introduction of outside aerosol particles into the cave.

  2. Constraining the subsoil carbon source to cave-air CO2 and speleothem calcite in central Texas

    Science.gov (United States)

    Bergel, Shelly J.; Carlson, Peter E.; Larson, Toti E.; Wood, Chris T.; Johnson, Kathleen R.; Banner, Jay L.; Breecker, Daniel O.

    2017-11-01

    Canonical models for speleothem formation and the subsurface carbon cycle invoke soil respiration as the dominant carbon source. However, evidence from some karst regions suggests that belowground CO2 originates from a deeper, older source. We therefore investigated the carbon sources to central Texas caves. Drip-water chemistry of two caves in central Texas implies equilibration with calcite at CO2 concentrations (PCO2_sat) higher than the maximum CO2 concentrations observed in overlying soils. This observation suggests that CO2 is added to waters after they percolate through the soils, which requires a subsoil carbon source. We directly evaluate the carbon isotope composition of the subsoil carbon source using δ13C measurements on cave-air CO2, which we independently demonstrate has little to no contribution from host rock carbon. We do so using the oxidative ratio, OR, defined as the number of moles of O2 consumed per mole of CO2 produced during respiration. However, additional belowground processes that affect O2 and CO2 concentrations, such as gas-water exchange and/or diffusion, may also influence the measured oxidative ratio, yielding an apparent OR (ORapparent). Cave air in Natural Bridge South Cavern has ORapparent values (1.09 ± 0.06) indistinguishable from those expected for respiration alone (1.08 ± 0.06). Pore space gases from soils above the cave have lower values (ORapparent = 0.67 ± 0.05) consistent with respiration and gas transport by diffusion. The simplest explanation for these observations is that cave air in NB South is influenced by respiration in open-system bedrock fractures such that neither diffusion nor exchange with water influence the composition of the cave air. The radiocarbon activities of NB South cave-air CO2 suggest the subsoil carbon source is hundreds of years old. The calculated δ13C values of the subsoil carbon source are consistent with tree-sourced carbon (perhaps decomposing root matter), the δ13C values of which

  3. Mineral magnetic environmental record in clastic cave deposits

    Czech Academy of Sciences Publication Activity Database

    Šlechta, Stanislav; Kadlec, Jaroslav

    2008-01-01

    Roč. 38, special issue (2008), s. 134-134 ISSN 1335-2806. [Paleo, Rock and Environmental Magnetism. Castle Meeting /11./. 22.06.2008-28.06.2008, Bojnice] Institutional research plan: CEZ:AV0Z30130516 Keywords : mineral magnetic environmental reconstruction * cave sediments * Moravian Karst Subject RIV: DE - Earth Magnetism, Geodesy, Geography

  4. The bat fauna of the Kararaô and Kararaô Novo caves in the area under the influence of the Belo Monte hydroelectric dam, in Pará, Brazil

    Directory of Open Access Journals (Sweden)

    M. Zortéa

    Full Text Available Abstract Brazil’s large territory displays significant richness in caves with about 12 thousand caves already recorded. Nevertheless, studies on bats in these environments are extremely scarce and fragmented. This study characterized the chiropteran fauna from two sandstone caves under the influence of the Belo Monte hydroelectric dam (Belo Monte UHE in Pará, Brazil. The Kararaô and Kararaô Novo caves are located on the same ridge, 250 m apart. Three expeditions were carried out in 2013 and 2014, with a 4- to 5-month interval in between. A total of 589 animals were caught, 246 in the Kararaô cave and 343 in the Kararaô Novo cave. Fifteen species were recorded (13 in each cave representing 79% similarity. With the exception of Vampyrum spectrum, which is not a cave species, the remaining recorded species were mostly cave bat species. Some species seemed to use the caves seasonally, although the basis of this pattern is still unknown. The most commonly observed species were Pteronotus personatus (dominant in the Kararaô cave, P. parnellii (dominant in the Kararaô Novo cave, and Lionycteris spurrelli, which accounted for 65% of all captures recorded for the two caves. Natalus macrourus is a species recorded in the Kararaô cave that is regionally threatened with extinction. Both caves are less than 500 m from the future reservoir; however, because the Kararaô cave entry is in an area that is lower than the reservoir, it can suffer alterations that would affect its dynamics. This raises great concern about the cave’s associated fauna.

  5. Hazard-evaluation and technical-assistance report No. TA-76-54, Department of the Interior, National Park Service: Cave study

    International Nuclear Information System (INIS)

    Gunter, E.P.; Meyer, C.R.

    1978-05-01

    In response to a request from the Department of the Interior, a medical evaluation was made of employees working in eight caves in the National Park System. Concern was directed at possible radon-daughter radiation exposures. Cave employees were given a questionnaire requesting past occupational exposure as well as past pulmonary history and tobacco consumption. Only a few of 35 comparisons within NIOSH had any consistent abnormality of significance. No widespread pulmonary cytological abnormalities were found in the cohort. The authors conclude that there is no apparent respiratory effect at the time. They recommend routine monitoring of cave employees older than 40 to 45 years of age who are cigarette smokers and who have worked in caves for more than 5 years. Those over 45 years of age and employed in caves for 5 years or more should receive high priority for monitoring regardless of smoking status. The authors recommend that all employees be encouraged to stop smoking, that a general preemployment physical be given including baseline sputum cytology and chest x-ray studies, that a yearly examination be given with laboratory studies on the aforementioned high risk individuals, and that cave air should not be used for air conditioning

  6. Control effect of fracture on hard coal cracking in a fully mechanized longwall top coal caving face

    Energy Technology Data Exchange (ETDEWEB)

    Jin-ping Wei; Zhong-hua Li; Pei-miao Sang; Shang-qiang Chen [Henan Polytechnic University, Jiaozuo (China). School of Energy Science and Engineering

    2009-03-15

    Through theoretical analysis, simulation test and practice, the law of a fracture's influence on hard top coal press cracking was studied. The study focused on the relation between fracture and coal strength, top coal caving ability and work face layout. Based on the investigation of the fracture system, the control of press cracking was achieved by matching working face to fracture orientation to improve top-coal caving ability and recovery. The matching principle was pointed out: the top-coal caving working face should be perpendicular to or obliquely cross the primary fracture at a large angle, and cross the secondary fracture at a small angle. The rational match can increase the recovery ratio of top-coal and avoid rib spalling. The application of control technology on hard top coal press cracking was introduced at the longwall top-coal caving face. 10 refs., 2 figs., 1 tab.

  7. The blurred line between photic and aphotic environments: a large Mexican cave with almost no dark zone

    Directory of Open Access Journals (Sweden)

    Luis M. Mejía-Ortíz

    2018-01-01

    Full Text Available Sistema Muévelo Rico is a 1.2 km long cave in Quintana Roo, less than 2 km from the Caribbean Sea. We measured illuminance to a level of 0.1 lux, organic matter (weight loss on ignition, temperature, and relative humidity. The last two were measured at hourly intervals for nearly one year. Approximately one-third of the cave has illuminance values greater than 0.01 lux, and most of the rest of the cave has light as well. Temperature and relative humidity were relatively constant, but they showed a daily cycle at all stations, albeit with different strengths. Organic matter averaged 8%, intermediate in value between surface and aphotic zones. Both eyeless species and eyed predators occurred throughout the cave. Their occurrence can best be explained by their foraging for organic matter and incurring an increased risk of predation.

  8. NAK WP-Cave project

    International Nuclear Information System (INIS)

    Svemar, C.

    1985-11-01

    WP-Cave is designed as an egg-shaped underground structure for intermediate storing and final disposal of high-level nuclear waste. Its height, when storing 1600 tonnes of spent fuel, is about 250 m and its diameter 110 m at mid-height. The waste storage has a compact layout and is surrounded by two engineered barriers. The innermost one is a 5 m-wide shield consisting of a mixture of bentonite clay and same which has a low hydraulic conductivity. This shield is surrounded by a so-called hydraulic cage, which initially drains the storage rock mass and, in the long-term diverts, the ground water flow past the storage. In this way an initial dry supervision period can be maintained. After sealing-off the storage and after water-filling, a stagnant chemical environment is established inside the bentonite-sand barrier preventing the disposed waste from being dissolved and from migrating to the geosphere. The programme, as outlined by the Project Board, has considered R and D questions with specific relation to the WP-Cave such as: properties of low-graded bentonite mixtures, function of the hydraulic cage, full-face boring of the storage, geomechanics of the storage and the bentonite-sand barrier, dry ventilation of the storage, temperature distributions and thermal stresses. An initial safety analysis has also been conducted. The hydraulic conductivity of low-grade bentonite mixtures has been measured in laboratory tests and found to be higher than expected. Tests of gas conductivity, for instance, confirm that only low gas pressures would build up inside the bentonite-sand barrier. The initial safety analysis indicates that a compact storage, such as that presented, would allow for the safe isolation of the spent nuclear fuel and would fulfull the radiation protection criterion of 0.1 mSv/year. With 27 refs. (Author)

  9. THE MOLLUSCAN FAUNA FROM THE UPPER PLEISTOCENE VERTEBRATE-BEARING DEPOSITS OF S. TEODORO CAVE (NORTH-EASTERN SICILY

    Directory of Open Access Journals (Sweden)

    DANIELA ESU

    2007-03-01

    Full Text Available This paper deals with terrestrial, freshwater and marine molluscs collected in the upper Pleistocene deposits of clay, sands and gravels of S. Teodoro Cave (North-eastern Sicily. Beginning from 1998 two trenches have been excavated (1998 and 2002-2004. A highly diversified assemblage of endemic and not endemic vertebrates (elephant, horse, wild ox, deer, wild boar, hyaena, fox, mouse, ground vole, shrew, hedgehog, bats, birds, reptiles, invertebrates (molluscs and vegetal remains have been collected from the two trenches. The molluscan fauna is represented by poor to rich-species assemblages of land and freshwater gastropods and bivalves with Mediterranean-European character. Some species have been found for the first time as fossils in Sicily. The land snails prevail in the 1998 trench showing a persistent arid environment during the time of the sediment deposition. The freshwater species, characteristic of slow-running water, point to the presence of a small water body (stream or spring inside the cave, probably more consistent in the 2002-2004 trench where this fauna prevails. The dispersal of the molluscan fauna of S. Teodoro Cave from the mainland during the low stand sea-level phases of the upper Pleistocene probably belongs to the same dispersal events following the Oxygen Isotope Stage 5e which introduced into the island not endemic faunal elements which are associated with endemic faunal elements in S. Teodoro Cave. Littoral marine reworked molluscs found in the cave deposits probably come from the sedimentary cover of a middle Pleistocene terrace which overlies the roof of the cave. SHORT NOTE

  10. Detecting and characterizing unroofed caves by ground penetrating radar

    Science.gov (United States)

    Čeru, Teja; Šegina, Ela; Knez, Martin; Benac, Čedomir; Gosar, Andrej

    2018-02-01

    The bare karst surface in the southeastern part of Krk Island (Croatia) is characterized by different surface karst features, such as valley-like shallow linear depressions and partially or fully sediment-filled depressions of various shapes and sizes. They were noticed due to locally increased thickness of sediment and enhanced vegetation but had not yet been systematically studied and defined. Considering only the geometry of the investigated surface features and the rare traces of cave environments detected by field surveys, it was unclear which processes (surface karstification and/or speleogenesis) contributed most to their formation. The low-frequency ground penetrating radar (GPR) method using a special 50 MHz RTA antenna was applied to study and describe these karst features. Three study sites were chosen and 5 km of GPR profiles were positioned to include various surface features. The results obtained from the GPR investigation lead to the following conclusions: (1) an increased thickness of sediment was detected in all the investigated depressions indicating their considerable depth; (2) areas between different depressions expressed as attenuated zones in GPR images reveal their interconnection; (3) transitions between surface and underground features are characterized by a collapsed passage visible in the GPR data; and (4) an underground continuation of surface valley-like depressions was detected, proving the speleogenetic origin of such features. Subsurface information obtained using GPR indicates that the valley-like depressions, irregular depressions completely or partially filled with sediment, and some dolines are associated with a nearly 4 km-long unroofed cave and developed as a result of karst denudation. In the regional context, these results suggest long-lasting karstification processes in the area, in contrast to the pre-karstic fluvial phase previously assumed to have occurred here. This research is the first application of the GPR method to

  11. Study of firedamp release in sub-level caving

    Energy Technology Data Exchange (ETDEWEB)

    Castano, F.S.; Sanz Delgado, M.A.; Iturriaga Trenor, S.; Alberdi Vinas, C.

    1988-01-01

    The field work developed in the pits of 'Hullera Vasco-Leonesa' in Santa Lucia (Leon) to control firedamp release is described. Conclusions obtained concerning gas emission, irregularity and firedamp balance in sub-level caving winning faces are discussed. Some definitions of basic concepts in firedamp studies are also included. 7 figs.

  12. Quaternary faulting in the Tatra Mountains, evidence from cave morphology and fault-slip analysis

    OpenAIRE

    Szczygieł Jacek

    2015-01-01

    Tectonically deformed cave passages in the Tatra Mts (Central Western Carpathians) indicate some fault activity during the Quaternary. Displacements occur in the youngest passages of the caves indicating (based on previous U-series dating of speleothems) an Eemian or younger age for those faults, and so one tectonic stage. On the basis of stress analysis and geomorphological observations, two different mechanisms are proposed as responsible for the development of these displacements. The firs...

  13. Evaluación de la actividad antioxidante bioautográfica de cinco variedades de aceites esenciales andinos (Aristeguietia glutinosa; Myrcianthes rhopaloides; Ambrosia arborescens; Lantana camara; Minthostachys mollis)

    OpenAIRE

    Guerrero Toapanta, Priscila Pamela; Pozo Morales, Karla Natalia

    2016-01-01

    This experimental work had the goal of assessing the antioxidant capabilities of five essential oils: Minthostachys mollis, Aristeguietia glutinosa, Ambrosia arborescens, Myrcianthes rhopaloides and Lantana camara; all of which were obtained from the Ecuadorian Andes. This work used the DPPH and ABTS methods to assess free radical-capturing capacity, and it used the β-carotene Test to determine the antioxidant activity of each essential oil. The natural referent used herein...

  14. Total selenium and selenium species in irrigation drain inflows to the Salton Sea, California, April and July 2007

    Science.gov (United States)

    May, Thomas W.; Walther, Michael J.; Saiki, Michael K.; Brumbaugh, William G.

    2007-01-01

    This report presents the results for two sampling periods during a 4-year monitoring survey to provide a characterization of selenium concentrations in selected irrigation drains flowing into the Salton Sea, California. Total selenium, selenium species, and total suspended solids were determined in water samples, and total selenium was determined in sediment, detritus, and biota that included algae, plankton, midge larvae (family, Chironomidae), and two fish species-western mosquitofish (Gambusia affinis), and sailfin molly (Poecilia latipinna). In addition, sediments were analyzed for percent total organic carbon and particle size. Total selenium concentrations in water for both sampling periods ranged from 1.43 to 47.1 micrograms per liter, predominately as selenate, which is typical of waters leached out of selenium-contaminated marine shales under alkaline and oxidizing conditions. Total selenium concentrations ranged from 0.88 to 20.2 micrograms per gram in biota, and from 0.15 to 28.9 micrograms per gram in detritus and sediment.

  15. A 230 ka record of glacial and interglacial events from Aurora Cave, Fiordland, New Zealand

    International Nuclear Information System (INIS)

    Williams, P.W.

    1996-01-01

    Caves overrun by glaciers are known to accumulate dateable evidence of past glacial and interglacial events. Results are reported from an investigation of Aurora Cave on the slopes above Lake Te Anau in Fiordland. The cave commenced to form before c. 230 ka B.P. Sequences of glaciofluvial sediments interbedded with speleothems are evidence of the number and timing of glacial advances and the status of intervals between them. Twenty-six uranium series dates on speleothems underpin a chronology of seven glacial advances in the last 230 ka, with the peak of the late Otira glaciation, Aurora 3 advance, at c. 19 ka B.P. With five advances in the Otiran, the last glaciation is more complex than previously recognised. Comparison of the record with that recorded offshore from DSDP Site 594 reveals little matching, but the correspondence of the Aurora sequence with that interpreted from other onshore deposits is more convincing. Glacial deposits on slopes above the cave for a further 660 m may be evidence of the 'missing' glacial events of the mid-early Pleistocene. (author). 44 refs., 12 figs., 5 tabs

  16. A diverse intrinsic antibiotic resistome from a cave bacterium.

    Science.gov (United States)

    Pawlowski, Andrew C; Wang, Wenliang; Koteva, Kalinka; Barton, Hazel A; McArthur, Andrew G; Wright, Gerard D

    2016-12-08

    Antibiotic resistance is ancient and widespread in environmental bacteria. These are therefore reservoirs of resistance elements and reflective of the natural history of antibiotics and resistance. In a previous study, we discovered that multi-drug resistance is common in bacteria isolated from Lechuguilla Cave, an underground ecosystem that has been isolated from the surface for over 4 Myr. Here we use whole-genome sequencing, functional genomics and biochemical assays to reveal the intrinsic resistome of Paenibacillus sp. LC231, a cave bacterial isolate that is resistant to most clinically used antibiotics. We systematically link resistance phenotype to genotype and in doing so, identify 18 chromosomal resistance elements, including five determinants without characterized homologues and three mechanisms not previously shown to be involved in antibiotic resistance. A resistome comparison across related surface Paenibacillus affirms the conservation of resistance over millions of years and establishes the longevity of these genes in this genus.

  17. Seven Possible Cave Skylights on Mars

    Science.gov (United States)

    2007-01-01

    [figure removed for brevity, see original site] Figure 1 Seven very dark holes on the north slope of a Martian volcano have been proposed as possible cave skylights, based on day-night temperature patterns suggesting they are openings to subsurface spaces. These six excerpts of images taken in visible-wavelength light by the Thermal Emission Imaging System camera on NASA's Mars Odyssey orbiter show the seven openings. Solar illumination comes from the left in each frame. The volcano is Arsia Mons, at 9 degrees south latitude, 239 degrees east longitude. The features have been given informal names to aid comparative discussion (see figure 1). They range in diameter from about 100 meters (328 feet) to about 225 meters (738 feet). The candidate cave skylights are (A) 'Dena,' (B) 'Chloe,' (C) 'Wendy,' (D) 'Annie,' (E) 'Abby' (left) and 'Nikki,' and (F) 'Jeanne.' Arrows signify north and the direction of illumination. Mars Odyssey is managed by NASA's Jet Propulsion Laboratory, a division of the California Institute of Technology, Pasadena, for NASA's Science Mission Directorate, Washington. Lockheed Martin Space Systems, Denver, is the prime contractor for the project and built the spacecraft. The orbiter's Thermal Emission Imaging System was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing, Santa Barbara, Calif., and is operated by Arizona State University.

  18. The effect of body size evolution and ecology on encephalization in cave bears and extant relatives.

    Science.gov (United States)

    Veitschegger, Kristof

    2017-06-05

    The evolution of larger brain volumes relative to body size in Mammalia is the subject of an extensive amount of research. Early on palaeontologists were interested in the brain of cave bears, Ursus spelaeus, and described its morphology and size. However, until now, it was not possible to compare the absolute or relative brain size in a phylogenetic context due to the lack of an established phylogeny, comparative material, and phylogenetic comparative methods. In recent years, many tools for comparing traits within phylogenies were developed and the phylogenetic position of cave bears was resolved based on nuclear as well as mtDNA. Cave bears exhibit significantly lower encephalization compared to their contemporary relatives and intraspecific brain mass variation remained rather small. Encephalization was correlated with the combined dormancy-diet score. Body size evolution was a main driver in the degree of encephalization in cave bears as it increased in a much higher pace than brain size. In Ursus spelaeus, brain and body size increase over time albeit differently paced. This rate pattern is different in the highest encephalized bear species within the dataset, Ursus malayanus. The brain size in this species increased while body size heavily decreased compared to its ancestral stage. Early on in the evolution of cave bears encephalization decreased making it one of the least encephalized bear species compared to extant and extinct members of Ursidae. The results give reason to suspect that as herbivorous animals, cave bears might have exhibited a physiological buffer strategy to survive the strong seasonality of their environment. Thus, brain size was probably affected by the negative trade-off with adipose tissue as well as diet. The decrease of relative brain size in the herbivorous Ursus spelaeus is the result of a considerable increase in body size possibly in combination with environmental conditions forcing them to rest during winters.

  19. Emergência e desenvolvimento inicial de sementes de Dimorphandra mollis Benth. em campo

    Directory of Open Access Journals (Sweden)

    M.F. SOUZA

    2016-03-01

    Full Text Available RESUMO Dimorphandra mollis Benth. (Leguminosae-Caesalpinioideae é uma espécie arbórea, encontrada no bioma Cerrado, utilizada na recuperação de áreas degradadas e na ornamentação. Dos frutos, extrai-se a rutina, um bioflavonoide utilizado na indústria farmacêutica, o qual atua na permeabilidade e na resistência dos vasos capilares. Esta pesquisa teve como objetivo verificar os efeitos do tipo de solo e de adubos sobre a emergência e o vigor da fava-d’anta semeada diretamente no campo. O delineamento experimental adotado foi em blocos ao acaso, em esquema fatorial 4 x 2 (quatro tipos de adubação e dois tipos de solo, com sete repetições de cinco sementes cada. Foram avaliadas as seguintes características: emergência, primeira contagem da emergência, índice de velocidade de emergência, altura e diâmetro da plântula. Não houve interação significativa entre os fatores, tipo de solo e adubos. Os melhores resultados para emergência e IVE são obtidos quando a semeadura é realizada em solo local.

  20. Ecological Aspects of Phlebotomine Sandflies (Diptera: Psychodidae) from a Cave of the Speleological Province of Bambuí, Brazil

    Science.gov (United States)

    Carvalho, Gustavo Mayr de Lima; Brazil, Reginaldo Peçanha; Ramos, Mariana Campos das Neves Farah; Serra e Meira, Paula Cavalcante Lamy; Zenóbio, Ana Paula Lusardo de Almeida; Botelho, Helbert Antônio; Sanguinette, Cristiani de Castilho; Saraiva, Lara; Andrade Filho, José Dilermando

    2013-01-01

    Phlebotomines are invertebrate hosts of Leishmania genus species which are etiological agents of leishmaniases in humans and other mammals. Sandflies are often collected in entomological studies of caves both in the inner area and the adjacent environments. Caves are ecotypes clearly different from the external environment. Several caves have been opened to public visitation before any studies were performed and the places do not have scientific monitoring of the fauna, flora, geological and geographical characteristics. These events can lead to the loss of geological and biological information. Considering these aspects, this study aimed to describe the sand fly fauna, including the ecological features, in a limestone cave at the Speleological Province of Bambuí (Minas Gerais State, Brazil). A total of 8,354 specimens of sandflies belonging to 29 species were analyzed: Lutzomyia cavernicola (20%), Nyssomyia intermedia (15%), Martinsmyia oliveirai (13%), Evandromyia spelunca (12%), Evandromyia sallesi (11%), Migonemyia migonei (9%), Nyssomyia whitmani (9%), Sciopemyia sordellii (4%) and Lutzomyia longipalpis (2%). The others species represent 5% of the total. This manuscript presents data found on richness, diversity, evenness and seasonality, comparing the sand fly fauna trapped in the cave and its surroundings. PMID:24130847

  1. Phlebotomine sand flies of edible-nest swiftlet cave of Lang Ga Jiew Island, Chumphon province, Thailand.

    Science.gov (United States)

    Chittsamart, B; Samruayphol, Suchada; Sungvorayothin, Sangsit; Pothiwat, Ratcharin; Samung, Yudthana; Apiwathnasorn, Chamnarn

    2015-09-01

    The present study reported for the first time phlebotomine sandfly species inhabiting edible-nest swiftlet cave of the isolated island, based on field collections made during June 2010-May 2011. The insect diversity was relatively lower to that of mainland caves. All species, Phlebotomus stantoni, Sergentomyia anodontis, Sergentomyia bailyi, Sergentomyia gemmea, Sergentomyia hodgsoni and Sergentomyia punjabensis were either endemic island species or native elsewhere in Thailand. Sergentomyia hodgsoni was the most prevalent species accounted for 94.7% and classified as a troglophile species. Seasonal pattern of the phlebotomine abundance and some aspects of their population characteristics were described and discussed. Two ectoparasites, Ornithodorus and Paracimex sp. were also incidentally collected from the swiftlet cave.

  2. Avifaunal changes revealed in Quaternary deposits near Waitomo Caves, North Island, New Zealand

    International Nuclear Information System (INIS)

    Worthy, T.H.; Swabey, E.J.

    2002-01-01

    Late Pleistocene (23,000-10,000 14 C yr BP) and Holocene (10,000 14 C yr - present BP) avifaunas are described from the Henry Lambert and associated passages in Gardners Gut Cave, Waitomo Caves, North Island, New Zealand. Nine radiocarbon dates on bones and three uranium series dates on speleothems support the dating of the sites. These data are augmented by six new dates and reanalysis of the avifaunas from F1c Cave, also in the Waitomo karst. A distinctive Pleistocene avifauna characterised by the moa Euryapterix curtus and Pachyornis mappini and the North Island goose (Cnemiornis gracilis) with common associates including New Zealand coot (Fulica prisca), North Island takahe (Porphyrio mantelli), Finsch's duck (Euryanas finschi), and North Island kokako (Callaeas wilsoni) was present. The presence of kokako, saddleback (Philesturnus rufusater), and robin (Petroica longipes) in the Pleistocene deposits and the absence of grassland taxa such as pipit (Anthus noveaseelandiae) and quail (Coturnix novaezelandiae) indicate that the vegetation about Gardners Gut Cave included tall shrubland and probably lacked much grassland, even over the Last Glacial Maximum. The Holocene at Waitomo was characterised by a moa fauna dominated by Anomalopteryx didiformis, although both Euryapteryx curtus and Pachyornis mappini persisted in small numbers. The frequent occurrence of kakapo (Strigops habroptilus), weka (Gallirallus australis), and brown teal (Anas chlorotis) typifies Holocene avifaunas; Cnemiornis and Fulica are unknown from Holocene deposits in the area. (author). 45 refs., 7 figs., 8 tabs

  3. Geomorphological map as a tool for visualisation of geodiversity - example from Cave Park Grabovaca (Croatia)

    Science.gov (United States)

    Buzjak, Nenad; Bocic, Neven; Pahernik, Mladen

    2014-05-01

    Cave Park Grabovaca is located near Perusic in Lika region (central Croatia). It was established in 2006 at the area of 5.95 km2 (protection category: significant landscape). The main task is management and protection of Samograd, Medina and Amidzina caves that were declared as geomorphological monuments, and 6 other caves located close to each other. Owing to the central geographic location in Croatian Dinaric karst area, good traffic connections between central Europe and tourist centres of the Adriatic coast, preserved nature and easy accessible karst features typical for the Dinaric Karst, it has good potential to develop as an research, educational and tourist centre. In 2013. Cave Park management and the Department of Geography (University of Zagreb, Faculty of Science) established a core team that started to develop the project of Geoeducational centre (GEC) with following goals: exploration-evaluation-presentation-education. According to the accepted strategy, the first step in the project process is to enlarge the area and change the protection category. During the consultation process team members take into account protection, environmental, local economy, tourism and local population issues and proposed that protected area should be increased to 52,2 km2. This enlargement provides more efficient protection, greater geodiversity and biodiversity by occupying geotope, biotope, and landscape units typical for the whole Lika karst region. The next step was inventorying, evaluation, analysis and visualisation of geological, geomorphological and speleological phenomena. This 2 year task was made in cooperation between Croatian Geomorphological Society, Department of Geography, Speleological Society Karlovac and Caving Club Samobor. The inventory was made using field-work mapping and geotagged photographs, cave mapping and DEM analysis. It resulted in GIS oriented geodatabase consisting of geomorphological forms, processes and cave inventory. From those data

  4. U-series dating and stable isotope records of speleothem records from the Scladina Cave (Belgium)

    Science.gov (United States)

    van der Lubbe, Jeroen; Bonjean, Dominique; Hellstrom, John; Verheyden, Sophie; Vonhof, Hubert

    2015-04-01

    The Scladina cave, situated in the village of Sclayn (Ardennes, Belgium) at the southern bank of the Meuse, is famous for its Neanderthal fossils and artefacts. The infilling of the cave consists of a succession of flowstone layers interbedded with reworked loess sediment from outside the cave. The younger flowstone layers correspond to interglacials MIS 5 and the Holocene, while the reworked loess sediments represent cooler conditions. By careful diagenetic screening, well-preserved speleothem material was selected for U-series dating and stable isotope analysis of calcite and fluid inclusions. The results provide important new constraints on the age of Neanderthal fossils and artefacts, and bracket the time periods with a hydroclimate favorable for speleothem growth. The combination of fluid inclusion and calcite isotope analysis documents climate variability in the interglacials at high temporal resolution.

  5. Cytotoxicity of the methanol extracts of Elephantopus mollis, Kalanchoe crenata and 4 other Cameroonian medicinal plants towards human carcinoma cells.

    Science.gov (United States)

    Kuete, Victor; Fokou, Fabrice W; Karaosmanoğlu, Oğuzhan; Beng, Veronique P; Sivas, Hülya

    2017-05-25

    Cancer still constitutes one of the major health concerns globally, causing serious threats on patients, their families, and the healthcare system. In this study, the cytotoxicity of the methanol extract of Elephantopus mollis whole plant (EMW), Enantia chlorantha bark (ECB), Kalanchoe crenata leaves (KCL), Lophira alata bark (LAB), Millettia macrophylla leaves (MML) and Phragmanthera capitata leaves (PCL) towards five human solid cancer cell lines and normal CRL2120 fibroblasts, was evaluated. Extracts were subjected to qualitative chemical screening of their secondary metabolite contents using standard methods. The cytotoxicity of samples was evaluated using neutral red uptake (NR) assay meanwhile caspase activation was detected by caspase-Glo assay. Flow cytometry was used to analyze the cell cycle distribution and the mitochondrial membrane potential (MMP) whilst spectrophotometry was used to measure the levels of reactive oxygen species (ROS). Phytochemical analysis revealed the presence of polyphenols, triterpenes and sterols in all extracts. The IC 50 values of the best samples ranged from 3.29 μg/mL (towards DLD-1 colorectal adenocarcinoma cells) to 24.38 μg/mL (against small lung cancer A549 cells) for EMW, from 2.33 μg/mL (mesothelioma SPC212 cells) to 28.96 μg/mL (HepG2 hepatocarcinoma) for KCL, and from 0.04 μg/mL (towards SPC212 cells) to 0.55 μg/mL (towards A549 cells) for doxorubicin. EMW induced apoptosis in MCF-7 cells mediated by MMP loss and increased ROS production whilst KCL induced apoptosis via ROS production. This study provides evidences of the cytotoxicity of the tested plant extract and highlights the good activity of Elephantopus mollis and Kalanchoe crenata. They deserve more exploration to develop novel cytotoxic drugs.

  6. Spatial distribution of soda straws growth rates of the Coufin Cave (Vercors, France

    Directory of Open Access Journals (Sweden)

    Perrette Yves

    2010-07-01

    Full Text Available The Choranche Cave system (Vercors, France is an excellent locality for measuring the growth rates of large numbers soda straws. This is especially the case for the Coufin Cave, as