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Sample records for catnip nepeta cataria

  1. Sustainable manufacture of insect repellents derived from Nepeta cataria.

    Science.gov (United States)

    Patience, Gregory S; Karirekinyana, Ginette; Galli, Federico; Patience, Nicolas A; Kubwabo, Cariton; Collin, Guy; Bizimana, Jean Claude; Boffito, Daria C

    2018-02-02

    Malaria devastates sub-Saharan Africa; the World Health Organization (WHO) estimates that 212 million people contract malaria annually and that the plasmodium virus will kill 419 000 in 2017. The disease affects rural populations who have the least economic means to fight it. Impregnated mosquito nets have reduced the mortality rate but the Anopheles mosquitoes are changing their feeding patterns and have become more active at dusk and early morning rather than after 22h00 as an adaptation to the nets. Everyone is susceptible to the Anopheles at these times but infants and pregnant women are the most vulnerable to the disease. Plant-based mosquito repellents are as effective as synthetic repellents that protect people from bites. They are sustainable preventative measures against malaria not only because of their efficacy but because the local population can produce and distribute them, which represents a source of economic growth for rural areas. Here, we extract and test the essential oil nepetalactone from Nepeta cataria via steam distillation. Families in endemic areas of Burundi found them effective against bites but commented that the odor was pungent. An epidemiological study is required to establish its clinical efficacy.

  2. Catnip essential oil as a barrier to subterranean termites (Isoptera: Rhinotermitidae) in the laboratory

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    C.J. Peterson; J. Ems-Wilson

    2003-01-01

    The essential oil of catnip, Nepeta cataria (Lamiacae) was evaluated for behavioral effects on two populations of subterranean termite, Reticulitermes flavipes (Kollar) and R. virginicus (Banks) (Isoptera: Rhinotermitidae). The catnip essential oil contained =36: 64 E,Z-nepetalactone and Z,E-nepetalactone,...

  3. Chemical Composition and Antimicrobial Activities of Essential Oils from Nepeta cataria L. against Common Causes of Food-Borne Infections

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    Zomorodian, Kamiar; Saharkhiz, Mohammad Jamal; Shariati, Samaneh; Pakshir, Keyvan; Rahimi, Mohammad Javad; Khashei, Reza

    2012-01-01

    Nepeta cataria L. is traditionally consumed as a food additive. The effects of three different harvest stages of N. cataria essential oils (EOs) against most common causes of food-borne infections were evaluated by broth microdilution method as recommended by the Clinical and Laboratory Standards Institute (CLSI). The chemical composition of the EOs from N. cataria has been analyzed by gas chromatography/mass spectrometry (GC/MS). The analysis of the EOs indicated that 4a-α,7-α,7a-β-nepetalactone (55–58%) and 4a-α,7-β,7a-α-nepetalactone (30–31.2%) were the major compounds of the EOs at all developmental stages. The results showed that the tested EOs exhibited antimicrobial activities against the food-borne pathogens at concentrations of 0.125–2 μL/mL. Based on these results, the EO of N. cataria can possibly be used in food products as a natural preservative agent. PMID:22779012

  4. Mechanism of modulation through PI3K-AKT pathway about Nepeta cataria L.'s extract in non-small cell lung cancer.

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    Fan, Jiaxin; Bao, Yongrui; Meng, Xiansheng; Wang, Shuai; Li, Tianjiao; Chang, Xin; Yang, Guanlin; Bo, Tao

    2017-05-09

    Non-small cell lung cancer (NSCLC) is regarded as one of the major intractable diseases, which was cured mainly by chemotherapeutics in the clinical treatment at present. But it is still a vital mission for the current medical and researchers that hunting a natural medicine which have little side effects and high-efficiency against the NSCLC on account of the shortcomings on current drugs. Nepeta cataria L. plays an important role in anti-cancer treatment according to the reports which was recorded in the Chinese Pharmacopoeia of version 2015 and belongs to one of the Traditional Chinese medicine (TCM). Microfluidic chip technology is widely used in scientific research field due to its high-throughput, high sensitivity and low cost with the continuous progress of science and technology. In this study, we investigate the effect of total flavonoid extracted from Nepeta cataria L. (TFS) through human lung cancer cell line A549 based on the microfluidic device and Flow Cytometry. So we detected the mRNA expression of MicroRNA-126 (miR-126), VEGF, PI3K, PTEN and proteins expression respectively to explore the partial PI3K-AKT pathway molecular mechanisms through Quantitative Real-time PCR (qRT-PCR) and Western Blot. The results showed that TFS can disturb the expression of miR-126 and regulate the PI3K-AKT signaling pathway to meet the effect of anti-cancer. Taking all these results into consideration we can draw a conclusion that TFS may be used as a novel therapeutic agent for NSCLC in the near future.

  5. Vapor pressure and enthalpy of vaporization of oil of catnip by correlation gas chromatography

    International Nuclear Information System (INIS)

    Simmons, Daniel; Gobble, Chase; Chickos, James

    2016-01-01

    Highlights: • Vaporization enthalpies of the nepetalactones from oil of catnip have been evaluated. • Vapor pressures from T = (298.15 to 350) K have been evaluated. • Oil of catnip has a vapor pressure similar to DEET at T = 298.15 K. - Abstract: The vaporization enthalpy and vapor pressure of the two nepetalactones found in Nepeta cataria have been evaluated by correlation gas chromatography. Vaporization enthalpies at T = 298.15 K of {(68.0 ± 1.9) and (69.4 ± 1.9)} kJ ⋅ mol"−"1 have been derived for the minor diastereomer, (4aS,7S,7aS)-nepetalactone, and major one, (4aS,7S,7aR)-nepetalactone, respectively. Vapor pressures also at T = 298.15 K of p = (1.2 ± 0.04) Pa and (0.91 ± 0.03) Pa have been evaluated for the minor and the major stereoisomer. In addition to being of interest because of the remarkable effect it has on various felids, oil of catnip is also quite effective in repelling mosquitoes, comparable to diethyl-m-toluamide (DEET). The vapor pressures evaluated in this work suggest that the two stereoisomers have similar volatility to DEET at ambient temperatures.

  6. Chemosensory responses to the repellent nepeta essential oil and its major component nepetalactone by the yellow fever mosquito, aedes aegypti, a vector of zika virus

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    Nepeta essential oil (Neo) (catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. However, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito Aedes aegypti, an important vector of...

  7. Elicitation effects of synthetic 1,2,4,5-tetraoxane and 2,5-diphenyltiophene in shoot cultures of two Nepeta species

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    Dmitrović Slavica

    2016-01-01

    Full Text Available The present study was aimed to investigate the elicitation possibility of the main secondary metabolites production in Nepeta cataria L. and N. pannonica L. plants, by exposing them to synthetic compounds from the group of tetraoxanes and tiophenes. The effect of DO63 (1,2,4,5-tetraoxane and DOVF15 (2,5-diphenyl-tiophene on the production of cis,trans-nepetalactone (NL and rosmarinic acid (RA in two Nepeta species, was investigated in shoots grown on culture medium with addition of synthetic compounds in concentrations ranging from 0.1 to 2 mg L-1. The content of targeted metabolites in tested in vitro shoots depended on the type and the concentration of applied synthetic compounds. Application of DO63, primarily in concentration of 0.1 mg L-1 to 1 mg L-1, affected only NL production in both Nepeta species resulting in increased NL content in treated shoots, while production of RA was not influenced. Addition of DOVF15 caused decrease of RA content in N. pannonica shoots and increase in N. cataria shoots, whereas NL production was not affected. The presented results highlight the possibility of DO63 and DOVF15 application for the elicitation of the main secondary metabolites production in species from the genus Nepeta. [Projekat Ministarstva nauke Republike Srbije, br. 172008 i br. 173024

  8. Antioxidant Flavonoids from Nepeta floccosa Benth.

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    Liaqat Ali

    2015-06-01

    Full Text Available Antioxidants are the substances which significantly delay or prevent the development of age-related degenerative and chronic diseases caused by oxidative damage. Due to this, there is a considerable interest in the identification of lead molecules, which can inhibit the oxidation process or slow it down considerably. Based on the spectrofluorimetric analysis of superoxide and diphenylpicrylhydrazyl radical scavenging ability, the bio-assay guided isolation of methanolic extract of Nepeta floccosa Benth. was carried out. As a result of this study, one new flavanonol named nepetavanol (1 and four known flavonoids; dihydrokaempferol (2, naringenin (3, caulesnarinside (4, and poncirin (5 have been isolated. The structure of the new flavanonol was assigned on the basis of 1H- and 13C-NMR spectra including two-dimensional NMR techniques such as COSY, HMQC, and HMBC experiments and FABMS, EIMS, HR-MS data. Compound 1 , 2, and 3 showed significant activity whereas compounds 4 and 5 showed moderate activity against antioxidant assays.

  9. Secondary metabolites from Nepeta juncea | Jamila | African Journal ...

    African Journals Online (AJOL)

    The study of the phytochemical investigation on the chemical constituents of the whole plant of Nepeta juncea belonging to the family Lamiaceae resulted in the isolation and characterization of 11 compounds. Nine of these compounds were identified as ursolic acid, oleanolic acid, β-sitosterol, stigmasterol, stigmasterol ...

  10. Constituent composition and biological activity of Nepeta manchuriensis essential oil

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    The essential oil present in the aerial parts of the plant Nepeta manchuriensis was prepared by steam distillation using clevenger apparatus. The chemical composition of the oil was studied by GCMS. Sabinene, elemol, selinene, 4-terpineol, menthatriene and neoisothujol are the major components and r...

  11. Insecticidal activities and phytochemical screening of crude extracts and its derived fractions from three medicinal plants Nepeta leavigata, Nepeta kurramensis and Rhynchosia reniformis

    International Nuclear Information System (INIS)

    Ahmad, N.; Shinwari, Z.K.

    2016-01-01

    The extracts and its derived fractions from three medicinal plants species Nepeta leavigata, Nepeta kurramensis and Rhynchosia reniformis were tested for insecticidal activities and preliminary phytochemical evaluation with the intention of standardization and proper manage of bioactive principles in such heterogonous botanicals and to encourage drug finding work with plants. The crude extracts and fractions from Nepeta plants showed moderate to strong insecticidal activity. Among the fractions from Nepeta kurramensis the n-butanol fraction showed strongest insecticidal activity with 89% mortality rate against Tribolium castaneum followed by methanol extract with 88% mortality ratio and in case of Nepeta leavigata the potential activity was showed by methanol extracts with 93% mortality rate against the tested insect. Surprisingly none of the extract / fractions obtained from Rhynchosia reniformis plant exhibited any insecticidal activity. The phytochemicals screening results revealed that both species of Nepeta showed similar phytochemicals profile. The group of chemicals terpenes, flavonoids and glycosides were observed in all the extracts/fractions of Nepeta plants. While phenolic compounds, acidic compounds and alkaloids were found in methanolic extracts, chloroform fraction and ethyl acetate fraction. The Rhynchosia reniformis was observed to be a good source of phenolic compounds, flavonoids, terpenes, alkaloids and fats. (author)

  12. Inhibition of Asthma in OVA Sensitized Mice Model by a Traditional Uygur Herb Nepeta bracteata Benth.

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    Jing Wang

    2016-01-01

    Full Text Available Asthma is a chronic lung inflammation which affects many people. As current therapies for asthma mainly rely on administration of glucocorticoids and have many side effects, new therapy is needed. In this study, we investigated Nepeta bracteata Benth., a traditional Uygur Herb, for its therapeutics effect in OVA induced asthmatic mice model. Treatment of OVA sensitized asthma mice with extract from Nepeta bracteata Benth. demonstrated improved lung pathology, as well as reduced infiltration of eosinophil and neutrophil. Nepeta bracteata Benth. extract also contributed to the rebalance of Th17/Treg cell via decreasing the Th17 cell and increasing the Treg, which was corresponding with the inhibited Th17 cytokine response and increased IL-10 level. Moreover, the reduced TGF-β level and Smad2/3 protein level also suggested that Nepeta bracteata Benth. extract could inhibit TGF-β mediated airway remodelling as well. Taken together, these data suggested that Nepeta bracteata Benth. may be a novel candidate for future antiasthma drug development.

  13. Calamintha nepeta (L.) Savi and its Main Essential Oil Constituent Pulegone: Biological Activities and Chemistry.

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    Božović, Mijat; Ragno, Rino

    2017-02-14

    Medicinal plants play an important role in the treatment of a wide range of diseases, even if their chemical constituents are not always completely recognized. Observations on their use and efficacy significantly contribute to the disclosure of their therapeutic properties. Calamintha nepeta (L.) Savi is an aromatic herb with a mint-oregano flavor, used in the Mediterranean areas as a traditional medicine. It has an extensive range of biological activities, including antimicrobial, antioxidant and anti-inflammatory, as well as anti-ulcer and insecticidal properties. This study aims to review the scientific findings and research reported to date on Calamintha nepeta (L.) Savi that prove many of the remarkable various biological actions, effects and some uses of this species as a source of bioactive natural compounds. On the other hand, pulegone, the major chemical constituent of Calamintha nepeta (L.) Savi essential oil, has been reported to exhibit numerous bioactivities in cells and animals. Thus, this integrated overview also surveys and interprets the present knowledge of chemistry and analysis of this oxygenated monoterpene, as well as its beneficial bioactivities. Areas for future research are suggested.

  14. Calamintha nepeta (L. Savi and its Main Essential Oil Constituent Pulegone: Biological Activities and Chemistry

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    Mijat Božović

    2017-02-01

    Full Text Available Medicinal plants play an important role in the treatment of a wide range of diseases, even if their chemical constituents are not always completely recognized. Observations on their use and efficacy significantly contribute to the disclosure of their therapeutic properties. Calamintha nepeta (L. Savi is an aromatic herb with a mint-oregano flavor, used in the Mediterranean areas as a traditional medicine. It has an extensive range of biological activities, including antimicrobial, antioxidant and anti-inflammatory, as well as anti-ulcer and insecticidal properties. This study aims to review the scientific findings and research reported to date on Calamintha nepeta (L. Savi that prove many of the remarkable various biological actions, effects and some uses of this species as a source of bioactive natural compounds. On the other hand, pulegone, the major chemical constituent of Calamintha nepeta (L. Savi essential oil, has been reported to exhibit numerous bioactivities in cells and animals. Thus, this integrated overview also surveys and interprets the present knowledge of chemistry and analysis of this oxygenated monoterpene, as well as its beneficial bioactivities. Areas for future research are suggested

  15. Differential effects of two strains of Rhizophagus intraradices on dry biomass and essential oil yield and composition in Calamintha nepeta

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    Roxana P Colombo

    Full Text Available The aim of this work was to determine the effects of two geographically different strains of Rhizophagus intraradices (M3 and GA5 on the total biomass and essential oil (EO yield and composition of Calamintha nepeta, with or without phosphorus (P fertilization, under greenhouse conditions.The plant biomass was not significantly affected by any of the treatments, showing higher values in control plants. Strains had a differential response in their root colonization rates: M3 reduced these parameters while GA5 did not modify them. Both strains affected EO yield in absence of P fertilization: M3 promoted EO yield in C. nepeta plants and GA5 resulted in negative effects. The percentage composition of EO was not significantly modified by either strain or P fertilization. M3 strain could be a potential fungal bioinoculant for production and commercialization of C. nepeta in the aromatic plant market.

  16. Chemical composition and antioxidant activity of phenolic compounds and essential oils from Calamintha nepeta L.

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    Khodja, Nabyla Khaled; Boulekbache, Lila; Chegdani, Fatima; Dahmani, Karima; Bennis, Faiza; Madani, Khodir

    2018-05-24

    Background Essential oils, infusion and decoction extracts of Calamintha nepeta L. were evaluated for their bioactive substances (polyphenols and essential oils) and antioxidant activities. Methods The amounts of phenolic compounds were determined by colorimetric assays and identified by high performance and liquid chromatography coupled with ultraviolet detector (HPLC-UV) method. The chemical composition of essential oils was determined by gas-chromatography coupled with mass spectrometry (GC/MS) method. For the evaluation of the antioxidant activity of essential oils and extracts, two different assays (reducing power and DPPH radical scavenging activity) were used. Results Infusion extract presented the highest phenolic content, followed by the decoction one, while the lowest amount was observed in essential oils. The amount of flavonoids of the decocted extract was higher than that of the infused one. The phenolic profile of C. nepeta infusion and decoction extracts revealed the presence of 28 and 13 peaks, respectively. Four phenolics compounds were identified in infusion (gallic acid (GA), rosmarinic acid (RA), caffeine (C) and caffeic acid (CA)) and two were identified in decoction (GA and RA). The chemical composition of essential oils revealed the presence of 29 compounds, accounting for the 99.7% of the total oils. Major compounds of essential oil (EO) were trans-menthone (50.06%) and pulegone (33.46%). Infusion and decoction extracts revealed an interesting antioxidant activity which correlates positively with their total phenolic contents. Conclusions These results showed that Calamintha nepeta could be considered as a valuable source of phenolics and essential oils with potent antioxidant activity.

  17. The Study on Diurnal Changes in Leaf Gas Exchange of Lemon Balm, Catnip, Holy Basil and Sweet basil in Ahvaz

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    Mohammad Mahmoodi Sourestani

    2017-02-01

    Full Text Available Introduction: Mediterranean climate conditions induce several stresses that plants have to cope with, especially during summer months when high temperature and radiation levels along with low water availability in the soil prevail for long periods. Variation in physiological traits such as photosynthesis and plant water status and their association with morphological characters can play an important role in the adaptability of the species to environmental constraints. The previous studies show that scorching weather not only affects the rate of gas exchange, but also results in diurnal changes in activity. Thus, the impact of environmental stresses on plants growing in these conditions should be assessed by examining the evolution of their diurnal variations on leaf gas exchange. Aromatic plants represent a renewable source of valuable compounds that can be used in food, perfumery, and pharmaceutical industry. Among these plants, sweet basil (Ocimumbasilicum, holy basil (Ocimum sanctum, lemon balm (Melissa officinalisL. and catnip (Nepetacataria are very important for different industries. Studies on environmental physiology of medicinal plants are relatively scarce and very few information is available concerning the physiological basis of medicinal plant response to heat stress that is one of the most important factors limiting production of medicinal plants in Khuzestan province. Material and methods: In order to evaluate the diurnal fluctuation of gas exchange of mentioned plants, an experiment was carried out in 2013 at research farm of Horticultural Science, Shahid Chamran University (31°20'N latitude and 48°40'E longitude and 22.5m mean sea level, Ahvaz (Iran, a site characterized by a semidry and scorching weather during late spring and summer. The experiment was arranged based on randomized complete block design (RCBD with three replications and 4×8 factorial scheme (Four plants including lemon balm, catnip, holy basil and basil; and

  18. In vitro cytotoxicity of allelopathic plants Adonis vernalis L. Origanum vulgare ssp. vulgare L. and Nepeta nuda subsp. nuda

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    Koleva, Vanya; Dragoeva, Asya; Stoyanova, Zheni; Yordanova, Zhenia; Ali, Selime; Uzunov, Nikolay M.; Melendez-Alafort, Laura; Rosato, Antonio; Enchev, Dobromir D.

    2018-03-01

    Medicinal plants produce various secondary metabolites as a part of their chemical defence and survival in nature. These compounds have a wide range of biological activities. Nowadays, medicinal plants are used as source of allelochemicals and new effective anticancer agents. Our previous studies revealed allelopathic potential of water extracts of Adonis vernalis L. (Ranunculaceae), Origanum vulgare ssp. vulgare L. and Nepeta nuda subsp. nuda (Lamiaceae). Present study aimed to evaluate the effect of the same extracts in vitro on human hepatoma cell line SK-HEP-1. Cell proliferation/viability was assessed using Premixed WST-1 Cell Proliferation Reagent. Adonis water extract (1.83mg/ml) had notable negative influence on cancer cell line tested. Oregano (3.5 mg/ml) also exerted negative effect, but to a lesser degree. On the contrary, nepeta water extract (6.59 mg/ml) had an opposite effect, stimulating cell proliferation. One possible explanation could be the type of extraction: after treatment with nepeta methanol extract (6.59 mg/ml) cell viability was significantly reduced. In conclusion, Adonis vernalis and Nepeta nuda subsp. nuda possess metabolites with growth inhibitory effect on human hepatoma cell line SK-HEP-1. Further research is needed to clarify biological activity of lower concentrations which are appropriate to enable the design of new anticancer drugs.

  19. Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents

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    Nestorović-Živković Jasmina

    2018-01-01

    Full Text Available Methanol extracts of three endemic Nepeta species were analyzed for their main secondary metabolites, terpenes and phenolics, and further investigated for antioxidant capacity and embryonic toxicity in zebrafish. UHPLC/DAD/(± HESI-MS/MS analysis showed that the dominant compound in N. rtanjensis was trans,cis-nepetalactone, the cis,trans isomer of this monoterpene lactone was dominant in N. sibirica, while nepetalactone was detected only in traces in N. nervosa. In all investigated species, rosmarinic acid was the dominant phenolic compound, while other identified phenolic acids (chlorogenic, neochlorogenic and caffeic were present in considerably lower amounts. ABTS and DPPH assays showed that the methanol extracts of N. rtanjensis, N. sibirica and especially N. nervosa possessed strong antioxidant activities, with the FRAP assay revealing high ferric-reducing abilities for all three tested species. Such a strong antioxidant potential, especially as manifested in the DPPH and FRAP assays, can be attributed to phenolic acids, and in the first place to rosmarinic acid. Increased lethality of zebrafish embryos in any of the treatments was not observed, but several toxic effects on embryonic development were recorded, such as pericardial and yolk sac edema. As in other Nepeta species, the three studied endemic species possessed a great potential for food conservation or as medicinal supplements if applied in optimized concentrations; however, alternative sources of plant material (e.g. field cultivation should be established bearing in mind their vulnerability in nature.

  20. Chemical composition and antibacterial activity of the essential oil the leaf of Nepeta persica

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    Soraya AKHSHI

    2014-11-01

    Full Text Available The essential oil from the leaf of Nepeta persica Boiss, analyzed by gas chromatography (GC and gas chromatography (GC/mass spectrometry (MS, were shown to contain 4aα, 7α, 7aβ-nepetalactone (49.46% and 4aα, 7α, 7aα-nepetalactone (14.18%. The other main constituents were n-octane (13.10%, n-decane (3.67% and germacrene-D (2.04%. Antibacterial activities of the leaf oil were evaluated using the micro-dilution broth method. Inhibitory effects on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Enterococcus faecalis were recorded. The leaf oil has difference activities against the test microorganisms. The antibacterial property of the essential oil might be ascribed to their high content of nepetalactone isomers.

  1. Some biological compounds, radical scavenging capacities and antimicrobial activities in the seeds of Nepeta italica L. and Sideritis montana L. sub sp. montana from Turkey

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    Emre, I.; Kursat, M.; Yilmaz, O.; Erecevit, P.

    2011-07-01

    This study determined some biological compounds (fatty acid compositions, lipid-soluble vitamins, sterols, flavonoids), radical scavenging capacities and antimicrobial activities in the seeds of Nepeta italica L. and Sideritis montana L. subsp. montana. It was found that palmitic acid (C16:0; 8.54+-0.13-3.05+-0.04%), oleic acid (C18:1 n9, 22.41+-0.8-18.83+-0.1%) and a-inolenic acid were the dominant fatty acids in both Nepeta italica L. and Sideritis montana L. subsp. montana. It was concluded that both Nepeta italica L. and Sideritis montana L. subsp. montana contained stigmasterol and ergosterol as well as beta-sitosterol. The present findings show that Nepeta italica L. contains morin, catechin, naringin and Sideritis montana L. subsp. montana contains morin, naringenin as major flavonoids. It was also determined that methanol extracts of Nepeta italica L. and Sideritis montana L. subsp. montana were most effective against DPPH radicals. The results of the present study show that the vitamins, flavonoids and fatty acid extracts in the seeds of N. italica L. and S. montana L. subsp. montana prevented the growth of the microorganisms used in the tests at different ratios. (Author).

  2. Antibacterial activity and composition of the essential oil of Nepeta hormozganica Jamzad from Iran.

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    Sonboli, A; Saadat, M H; Arman, M; Kanani, M R

    2017-12-01

    The composition and antibacterial activity of the essential oil of the aerial flowering parts of Nepeta hormozganica Jamzad have been studied. Analysis of the oil was conducted by GC-FID and GC-MS. Thirty-two components were characterized accounting for 99.4% of the total oil. Oxygenated monoterpenes (87.5%) were found to be the predominant group of compounds, of which 18-cineole (65.0%) and 4aα-7α-7aβ-nepetalactone (13.0%) were the main constituents. The antibacterial activity of the essential oil and its main constituents showed that all of the tested microorganisms were highly inhibited by the essential oil with inhibition zones ranged from 12 to 24 mm. The most sensitive bacteria were Staphylococcus aureus and Staphylococcus epidermidis with the lowest MIC values of 0.3 and 0.6 mg/mL, respectively. Considering sensitivity screening it is conceivable that the activity of the oil from N. hormozganica could be attributed mainly to the synergistic property of 18-cineole and nepetalactone.

  3. Effectiveness of Cuscuta planiflora Ten. and Nepeta menthoides Boiss. & Buhse in major depression: a triple-blind randomized controlled trial study.

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    Firoozabadi, Ali; Zarshenas, Mohammad M; Salehi, Alireza; Jahanbin, Saye; Mohagheghzadeh, Abdolali

    2015-04-01

    Depression is one the most common mental disorders that can be seen all over the world. In traditional Persian medicine, some medicinal herbs are recommended for depression treatment. This study aimed to evaluate the effects of Cuscuta planiflora Ten. and Nepeta menthoides Boiss. & Buhse in patients with major depression. This study is a randomized triple-blind controlled clinical trial conducted in the year 2010 in Shiraz University of Medical Sciences on patients with major depression. Pharmaceutical capsules of Cuscuta planiflora (500 mg) and Nepeta menthoides (400 mg) were prepared by a pharmacist. Patients were randomly assigned to 3 groups: group A (treated with Nepeta menthoides capsules and conventional drugs), group B (treated with Cuscuta planiflora capsules and conventional drugs), and group C (treated only with conventional drugs). The study period was 8 weeks and depression was measured before and after the study by Beck Depression Inventory and Hamilton Depression Inventory. The data were analyzed by SPSS version 20 and the P Cuscuta planiflora and Nepeta menthoides capsules could be effective, affordable herbal medicines with improved cost-benefit in treatment of major depression and it is worth designing further and more extensive studies to get to a more accurate conclusion. © The Author(s) 2014.

  4. Effect of Nepeta bracteata Benth. on allergic rhinitis symptoms: A randomized double-blind clinical trial

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    Mohammad Reza Hajiheydari

    2017-01-01

    Full Text Available Background: Allergic rhinitis (AR is one of the health problems in the world. It is necessary to develop new treatment procedure for control of this disease. The aim of this study was to assess the effect of Zofa (Nepeta bracteata Benth on AR patients. Materials and Methods: In this double-blind randomized clinical trial study, 71 patients (37 patients in treatment and 34 in placebo group participated. In treatment group, N. bracteata syrup (NBS was used for 4 weeks as three times a day. The efficacy of the drug regarding AR symptoms (rhinorrhea, sneezing, nasal obstruction, itchy nose, and ocular symptoms were evaluated through a visual analog scale (VAS by 0–10 before administration and at the end of the whole treatment period. The collected information was entered in the SPSS software (version 18 and was analyzed using the Fisher's exact test, Chi-square test, independent sample t-test, and paired sample test. Results: The improvement of AR symptoms in the group receiving NBS was significantly higher compared to control group (4.73 ± 1.84 vs. 0.38 ± 2.06; P < 0.0001. Furthermore, the mean of total VAS before and after the treatment (in case group was 7.10 ± 1.92 and 2.37 ± 1.76, respectively (P < 0.001. Conclusion: The results of this study indicate that N. bracteata has significant effects on improving the symptoms of AR. Hence, it can be a good alternative to AR symptoms relief.

  5. Phytochemical screening, total phenolic contents and biological evaluation of aerial parts of nepeta praetervisa

    International Nuclear Information System (INIS)

    Fareed, G.; Afza, N.; Mali, A.; Fareed, N.; Lateef, M.; Iqbal, L.; Mughal, U.R

    2013-01-01

    This study was designed to explore the phytochemical screening, total phenolic contents, radical scavenging potential and urease inhibitory activities in various fractions of the aerial parts of Nepeta praetervisa. Sub-fractions (n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous) were prepared from the crude methanolic extract using partition chromatography. Phytochemical tests were performed and revealed the presence of various classes of secondary metabolites in various sub-fractions (Table-1). Total phenolic contents of all the fractions were determined using Folin-Ciocalteu (FC) reagent and the ethyl acetate sub-fraction was found to possess the highest level of phenolic contents (627.25 mg gallic acid equivalent (GAE)/g) as compared to the other fractions. The radical scavenging activity was determined at various concentrations ranging from 2.5 - 0.15 micro g /10 mu L by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) method. At the lowest concentration level, the ethyl acetate sub-fraction showed maximum level of antioxidant activity (78%) compared to BHA used as standard. The decreasing order of activity was ethyl acetate>chloroform>aqueous>n-butanol>methanol>n-hexane. On the other hand when all these fractions were screened for urease inhibition activity using indophenols method, the ethyl acetate sub-fraction showed significant urease inhibitory activity (68 %) compared with the standard thiourea at the concentration of 50 mu g /10 mu L. The decreasing order of activity of various sub-fractions was ethyl acetate>chloroform>hexane>aqueous, while n-butanol sub- fraction was inactive. (author)

  6. A mechanistic evaluation of the traditional uses of Nepeta ruderalis in gastrointestinal and airway disorders.

    Science.gov (United States)

    Mahmood, Hassan; Chaudhry, Mueen Ahmad; Masood, Zeeshan; Saeed, Muhammad Asad; Adnan, Sherjeel

    2017-12-01

    Nepeta ruderalis Buch.-Ham. (Lamiaceae), locally known as Badranj Boya, is an aromatic herb used traditionally as an antispasmodic, antidiarrhoeal, and anti-asthamatic remedy. Aqueous methanolic extract of N. ruderalis was studied to investigate its traditional uses. Study was conducted from September 2015 to February 2016. In vitro spasmolytic and broncho-relaxant activity of crude extract of N. ruderalis (whole plant) was evaluated at 0.01-10 mg/mL final bath concentration in isolated rabbit jejunum and tracheal tissues, using PowerLab data acquisition system (Transonic Systems Inc., Ithaca, NY). In vivo antidiarrhoeal activity was evaluated in castor oil-induced diarrhoeal mice at the dose of 300 and 500 mg of crude extract orally. Crude extract of N. ruderalis completely relaxed spontaneously contracting, high K + (80 mM) and carbachol (1 μM) induced contracted jejunum with an EC 50 value of 5.85 (5.45-6.27), 4.0 (3.80-4.23) and 2.86 (2.48-3.29), similar to verapamil. Nr.Cr relaxed high K + and carbachol induced contractions, at 5 and 10 mg/mL with an EC 50 value of 2.37 (2.11-2.67) and 3.26 (2.9-3.67), respectively, and also displaced calcium concentration-response curves toward right at 0.1 and 0.3 mg/mL. Nr.Cr exhibited antidiarrhoeal protection at a dose of 300 and 500 mg/kg, similar to verapamil, whereas no acute toxicity signs were seen up to 5 g/kg in healthy mice. Results suggest the presence of spasmolytic and broncho-relaxant effects in the crude extract of N. ruderalis, possibly mediated through calcium channel-blocking activity, providing the pharmacological basis for its traditional uses in gastrointestinal and airway disorders.

  7. Chemical composition and biological activities of the essential oil from Calamintha nepeta plants from the wild in southern Italy.

    Science.gov (United States)

    Mancini, Emilia; De Martino, Laura; Malova, Hanna; De Feo, Vincenzo

    2013-01-01

    The chemical composition of the essential oil from aerial parts of Calamintha nepeta (L.) Savi, collected in the Campania Region, was obtained by hydrodistillation and analyzed by GC and GC-MS. In all, 42 compounds were identified. The oil was characterized by a prevalence of the sesquiterpenic fraction (80.8%). The main components were 1,10-di-epi-cubenol (18.5%), allo-aromadendrene epoxide (11.4%) and cadalene (5.7%). The essential oil was evaluated for its in vitro potential phytotoxic activity against germination and initial radical growth of Raphanus sativus L., Lepidium sativum L., Sinapis arvensis L., Triticum durum L. and Phalaris canariensis L. seeds. The essential oil showed no antioxidant activity.

  8. Some Biological Compounds, Radical Scavenging Capacities and Antimicrobial Activities in the seeds of Nepeta italica L. and Sideritis montana L. subsp. montana from Turkey

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    Erecevit, Pınar

    2011-03-01

    Full Text Available This study determined some biological compounds (fatty acid compositions, lipid-soluble vitamins, sterols, flavonoids, radical scavenging capacities and antimicrobial activities in the seeds of Nepeta italica L. and Sideritis montana L. subsp. montana. It was found that palmitic acid (C16:0; 8.54±0.13- 3.05±0.04%, oleic acid (C18:1 n9, 22.41±0.8-18.83±0.1% and α-linolenic acid (C18:3 n3;39.56±0.67-77.04±2.07% were the dominant fatty acids in both Nepeta italica L. and Sideritis montana L. subsp. montana. It was concluded that both Nepeta italica L. and Sideritis montana L. subsp. montana contained stigmasterol (630.07±1.81µg/g, 80.74±0.71µg/g, respectively and ergosterol (1.11±0.14µg/g, 161.32±0.63µg/g respectively as well as beta-sitosterol (2.93±0.03 µg/g. The present findings show that Nepeta italica L. contains morin (37.79±1.09μg/g, catechin (124.39±2.23µg/g, naringin (475.96±3.57µg/g and Sideritis montana L. subsp. montana contains morin (188.41±2.53µg/g, catechin (64.14±1.86μg/g, naringenin (38.34±1.78μg/g as major flavonoids. It was also determined that methanol extracts of Nepeta italica L. and Sideritis montana L. subsp. montana were most effective against DPPH radicals. The results of the present study show that the vitamins, flavonoids and fatty acid extracts in the seeds of N. italica L. and S. montana L. subsp. montana prevented the growth of the microorganisms used in the tests at different ratios.Este estudio ha determinado algunos compuestos biológicos (ácidos grasos, vitaminas liposolubles, esteroles y flavonoides, capacidad atrapadora de radicales libres, y actividades antimicrobianas de las semillas de Nepeta italica L. y Sideritis montana L. subsp. montana. Se encontró que el ácido palmítico (C16:0; 8.54±0.13-3.05±0.04%, ácido oleico (C18:1 n9, 22.41±0.8-18.83±0.1% y α-linolénico (C18:3 n 3;39.56±0.67-77.04±2.07% eran mayoritarios en ambas semillas de Nepeta italica L. y Sideritis

  9. In vitro antifungal and demelanizing activity of Nepeta rtanjensis essential oil against the human pathogen Bipolaris spicifera

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    Ljaljević-Grbić Milica

    2011-01-01

    Full Text Available The antifungal activity of Nepeta rtanjensis Diklić & Milojević essential oil was tested against the human pathogenic fungus Bipolaris spicifera (Bainier Subramanian via mycelial growth assay and conidia germination assay. The minimally inhibitory concentration (MIC of the oil was determined at 1.0 μg ml-1, while the MIC for the antifungal drug Bifonazole in a positive control was determined at 10.0 μg ml-1. The maximum of conidia germination inhibition was accomplished at 0.6 μg ml-1. In addition, at 0.6 μg ml-1 and 0.8 μg ml-1 the oil was able to cause morphophysiological changes in B. spicifera. The most significant result is the bleaching effect of the melanized conidial apparatus of the test fungi, since the melanin is the virulence factor in human pathogenic fungi. These results showed the strong antifungal properties of N. rtanjensis essential oil, supporting its possible rational use as an alternative source of new antifungal compounds.

  10. Contribución al estudio cariológico del género Nepeta l. en la Península Ibérica

    OpenAIRE

    Ubera, Jose L.

    1982-01-01

    En el presente trabajo, se estudian cariológicamente 14 taxones del género Nepeta en la Península Ibérica. Son nuevos, al parecer, los siguientes números cromosómios: N. nepetella subsp. cordifolia, n = 17; N, nepetalla subsp. aragonensis, n = 17; N. amethystina subsp. mallophora, n= 17, 2n = 34; N. multibrateata, n = 9, 2n= 18; N. tuberosa subsp. tuberosa. n = 9,2n = 18; N. tuberosa subsp. reticulata, n = 9, 2n = 18; N. granatensis n = 18, 2n = 36; N. Latiha, n = 18; N. coerulea, n =...

  11. Anticancer Activity of Chloroform Extract and Sub-fractions of Nepeta deflersiana on Human Breast and Lung Cancer Cells: An In vitro Cytotoxicity Assessment.

    Science.gov (United States)

    Al-Oqail, Mai M; Al-Sheddi, Ebtesam S; Siddiqui, Maqsood A; Musarrat, Javed; Al-Khedhairy, Abdulaziz A; Farshori, Nida N

    2015-10-01

    Cancer is one of the major causes of death worldwide. The plant-derived natural products have received considerable attention in recent years due to their diverse pharmacological properties including anticancer effects. Nepeta deflersiana (ND) is used in the folk medicine as antiseptic, carminative, antimicrobial, antioxidant, and for treating rheumatic disorders. However, the anticancer activity of ND chloroform extract has not been explored so far. The present study was aimed to investigate the anticancer activities of chloroform Nepeta deflersiana extract and various sub-fractions (ND-1-ND-15) of ND against human breast cancer cells (MCF-7) and human lung cancer cells (A-549). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and neutral red uptake assays, and cellular morphological alterations using phase contrast light microscope were studied. Cells were exposed with 10-1000 μg/ml of sub-fractions of ND for 24 h. Results showed that selected sub-fractions of the chloroform extract significantly reduced the cell viability of MCF-7 and A-549 cells, and altered the cellular morphology in a concentration-dependent manner. Among the sub-fractions, ND-10 fraction showed relatively higher cytotoxicity compared to other fractions whereas, ND-1 did not cause any cytotoxicity even at higher concentrations. The A-549 cells were found to be more sensitive to growth inhibition by all the extracts as compared to the MCF-7 cells. The present study provides preliminary screening of anticancer activities of chloroform extract and sub-fractions of ND, which can be further used for the development of a potential therapeutic anticancer agent. Nepeta deflersiana extract exhibit cytotoxicity and altered the cellular morphology. Sub-fractions of the chloroform extract of Nepeta deflersiana reduced the cell viability of MCF-7 and A-549 cells. Among the sub-fractions, ND-10 fraction showed relatively higher cytotoxicity. The A-549 cells were found to be more sensitive

  12. Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia

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    Alireza Mousavi Nia

    2017-03-01

    Full Text Available Background: Nepeta dschuparensis Bornm (NP is used as a medicinal herb in Iran. In traditional medicine, this herb is extensively employed for curing ailments such as cardiovascular diseases. NP has antioxidant and anti-inflammatory properties. This project examined the effects of the NP extract on cyclooxygenase-2 (COX-2 and interleukin-1β (IL-1β protein levels and its efficacy in neuroprotection in a cerebral ischemia-reperfusion model. Methods: Twenty-six male rats were randomly divided into 3 groups: 1 sham (n=6: no middle cerebral artery occlusion (MCAO procedure, 2 control (n=10: MCAO procedure and treatment with normal saline, and 3 NP extract (n=10: MCAO procedure and treatment with the NP extract (20 mg/kg, i.p. at the beginning of reperfusion. To examine the injury caused by cerebral ischemia, we measured motor coordination and the infarct area using the rotarod test and triphenyl tetrazolium chloride staining, respectively. IL-1β and COX-2 protein levels, as inflammatory markers, were measured by immunoblotting assay. The statistical analyses were performed using SPSS, version 16, and the data are expressed as means±SEMs. Statistical difference was evaluated using the one-way ANOVA, followed by the post hoc LSD test (P<0.01. Results: Treatment with the NP extract significantly diminished the infarct volume and alleviated the motor coordination disorder induced by cerebral ischemia. The NP extract administration significantly attenuated the increase in IL-1β and COX-2 protein levels too (P<0.01. Conclusion: The beneficial effects of the NP extract are related to its ability to decrease the levels of IL-1β and COX-2.

  13. Growth inhibition of human breast cancer cells and down-regulation of ODC1 and ADA genes by Nepeta binaloudensis

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    Akbar Safipour Afshar

    Full Text Available ABSTRACT Nepeta binaloudensis Jamzad, Lamiaceae, is a rare medicinal plant endemic to Iran. In spite of many studies about the chemical constituents and antibacterial effects of this species, no report has been provided about its cytotoxic and anticancer activities. In this study we have evaluated the effects of EtOH 70%, hexane and aqueous extracts of N. binaloudensis on the cell proliferation and n-hexane extract on the expression of adenosine deaminase and ornithine decarboxylase 1 genes in breast cancer cell lines (MCF-7, MDA-MB-231 compared to non-cancer line (MCF-10A. The cell lines were subjected to increasing doses of the extracts ranging from 10 to 320 µg/ml. Cell viability was quantified by MTS assay. Expression of adenosine deaminase and ornithine decarboxylase 1 genes was analyzed by real time PCR. N. binaloudensis inhibited the growth of malignant cells in a time and dose-dependent manner. Among extracts of N. binaloudensis, the hexane extract was found to be more toxic compared to other extracts. Results showed a marked decrease in the expression of ornithine decarboxylase 1 and adenosine deaminase genes in cancer cell lines. At 60 µg/ml concentration of N. binaloudensis hexane extract ornithine decarboxylase 1 and adenosine deaminase mRNA expression were reduced 4.9 fold and 3.5 fold in MCF-7 cell line and 3.6 fold and 2.6 fold in MDA-MB-231 cell line compared to control, respectively. The result of our study highlights the potential influences of N. binaloudensis hexane extract on ornithine decarboxylase 1 and adenosine deaminase genes expression in breast cancer cells and its relation to inhibition of cancer cell growth.

  14. Essential Oil Extraction, Chemical Analysis and Anti-Candida Activity of Calamintha nepeta (L. Savi subsp. glandulosa (Req. Ball—New Approaches

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    Mijat Božović

    2017-01-01

    Full Text Available A comprehensive study on essential oils extracted from different Calamintha nepeta (L. Savi subsp. glandulosa (Req. Ball samples from Tarquinia (Italy is reported. In this study, the 24-h steam distillation procedure for essential oil preparation, in terms of different harvesting and extraction times, was applied. The Gas chromatography–mass spectrometry (GC/MS analysis showed that C. nepeta (L. Savi subsp. glandulosa (Req. Ball essential oils from Tarquinia belong to the pulegone-rich chemotype. The analysis of 44 samples revealed that along with pulegone, some other chemicals may participate in exerting the related antifungal activity. The results indicated that for higher activity, the essential oils should be produced with at least a 6-h steam distillation process. Even though it is not so dependent on the period of harvesting, it could be recommended not to harvest the plant in the fruiting stage, since no significant antifungal effect was shown. The maximum essential oil yield was obtained in August, with the highest pulegone percentage. To obtain the oil with a higher content of menthone, September and October should be considered as the optimal periods. Regarding the extraction duration, vegetative stage material gives the oil in the first 3 h, while material from the reproductive phase should be extracted at least at 6 or even 12 h.

  15. Phytotoxic Effects of Nepeta meyeri Benth. Extracts and Essential Oil on Seed Germinations and Seedling Growths of Four Weed Species

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    Saban Kordali

    2015-05-01

    Full Text Available Essential oil isolated from the aerial parts of Nepeta meyeri Benth. by hydrodistilation was analysed by GC and GC-MS methods. A total 18 components were identified in the oil representing 100.0% of the oil. Main components were 4aα,7α,7aβ-nepetalactone (80.3%, 4aα,7α,7aα–nepetalactone (10.3%, trans-pulegol (3.1%, 1, 8-cineole (3.0% and β-bourbonene (2.0%. In addition, n-hexane extract of N. meyeri was analysed by using GC and GC-MS methods and 18 components were identified. Likewise, nepetalactones, 4aα,7α,7aβ-nepetalactone (83.7%, 4aα,7α,7aα–nepetalactone (3.6%, 1, 8-cineole (1.9% and α-terpinene (1.5% were the predominat compounds in the hexane extract. Three concentrations (0.5, 1.0 and 2.0 mg/mL of the essential oil and n-hexane, chloroform, acetone and methanol extracts isolated from the aerial partsand roots were tested for the herbicidal effects on the germination of the seeds of four weed species including Amaranthus retroflexus L., Chenopodium album L., Cirsium arvense L. and Sinapsis arvensis L. The essential oil of N. meyeri completely inhibited the germination of all weed seeds whereas the extracts showed various inhibition effects on the germination of the weed species. Herbicidal effect was increased with the increasing application concentrations of the extracts. In general, the acetone extract was found to be more effective as compared to the other extracts. All extracts also exhibited various inhibition effects on the seedling growths of the weed species. All extracts also tested for their phytotoxic effects on the weeds at greenhouse condition and the results showed that the oil and extracts caused mortality with 22.00-66.00% 48h after the treatments. These findings suggest that the essential oil and the extracts of N. meyeri have potentials for use as herbicides against those weed species.

  16. Effect of synthetic and plant-extracted aphid pheromones on the behaviour of Aphidius colemani

    Czech Academy of Sciences Publication Activity Database

    Ameixa, Olga; Kindlmann, Pavel

    2012-01-01

    Roč. 136, č. 4 (2012), s. 292-301 ISSN 0931-2048 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0073; GA MŠk LC06073 Institutional research plan: CEZ:AV0Z60870520 Keywords : (E)-b-farnesene * Aphidius colemani * Nepeta cataria oil * synthetic nepetalactone Subject RIV: EH - Ecology, Behaviour Impact factor: 1.560, year: 2012

  17. The perspective crops for the bioregenerative human life support systems

    Science.gov (United States)

    Polonskiy, Vadim; Polonskaya, Janna

    The perspective crops for the bioregenerative human life support systems V.I. Polonskiy, J.E. Polonskaya aKrasnoyarsk State Agrarian University, 660049, Krasnoyarsk, Russia In the nearest future the space missions will be too long. In this case it is necessary to provide the crew by vitamins, antioxidants, and water-soluble dietary fibers. These compounds will be produced by higher plants. There was not enough attention at present to increasing content of micronutrients in edible parts of crops candidates for CELSS. We suggested to add the new crops to this list. 1. Barley -is the best crop for including to food crops (wheat, rice, soybean). Many of the health effects of barley are connected to dietary fibers beta-glucan of barley grains. Bar-ley is the only seed from cereals including wheat with content of all eight tocopherols (vitamin E, important antioxidant). Barley grains contain much greater amounts of phenolic compounds (potential antioxidant activities) than other cereal grains. Considerable focus is on supplement-ing wheat-based breads with barley to introduce the inherent nutritional advantages of barley flour, currently only 20We have selected and tested during 5 generations two high productive barley lines -1-K-O and 25-K-O. Our investigations (special breeding program for improving grain quality of barley) are in progress. 2. Volatile crops. Young leaves and shoots of these crops are edible and have a piquant taste. A lot of organic volatile compounds, oils, vitamins, antioxidants are in their biomass. These micronutrients are useful for good appetite and health of the crew. We have investigated 11 species: basil (Ocimum basilicum), hyssop (Hyssopus officinalis), marjoram (Origanum majorana), sweet-Mary (Melissa officinalis), common thyme (Thymus vulgaris), creeping thyme (Thymus serpyllum), summer savory (Satureja hortensis), catnip (Nepeta cataria), rue (Ruta graveolens), coriander (Coriandrum Ativum), sulfurwort (Levisticum officinale). These

  18. Evaluation of macro and microminerals in crude drugs and infusions of five herbs widely used as sedatives

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    Marta E. Petenatti

    2011-07-01

    Full Text Available It has been determined the concentration of fourteen micro and macrominerals (Al, Ca, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Na, P, Se, and Zn in both crude drugs and infusions of Melissa officinalis L., Lamiaceae, Nepeta cataria L., Lamiaceae, Passiflora caerulea L., Passifloraceae, Tilia x moltkei Späth ex C.K. Schneid., Tiliaceae, and Valeriana officinalis L., Caprifoliaceae. These herbs are widely consumed by its sedative properties, either alone or in herb mixtures. All measurements were performed using an inductively coupled plasma optical emission spectrometer (ICP-OES. The products were obtained from regional markets, mainly in San Luis province (Argentina. The estimated daily intake was compared with current recommendations. All products and its infusions were included within the upper tolerable limits for minerals, in trace elements such as toxic elements present at low levels.

  19. Evaluation of macro and microminerals in crude drugs and infusions of five herbs widely used as sedatives

    Directory of Open Access Journals (Sweden)

    Marta E. Petenatti

    2011-12-01

    Full Text Available It has been determined the concentration of fourteen micro and macrominerals (Al, Ca, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Na, P, Se, and Zn in both crude drugs and infusions of Melissa officinalis L., Lamiaceae, Nepeta cataria L., Lamiaceae, Passiflora caerulea L., Passifloraceae, Tilia x moltkei Späth ex C.K. Schneid., Tiliaceae, and Valeriana officinalis L., Caprifoliaceae. These herbs are widely consumed by its sedative properties, either alone or in herb mixtures. All measurements were performed using an inductively coupled plasma optical emission spectrometer (ICP-OES. The products were obtained from regional markets, mainly in San Luis province (Argentina. The estimated daily intake was compared with current recommendations. All products and its infusions were included within the upper tolerable limits for minerals, in trace elements such as toxic elements present at low levels.

  20. Quantification of Heavy Metals in Mining Affected Soil and Their Bioaccumulation in Native Plant Species.

    Science.gov (United States)

    Nawab, Javed; Khan, Sardar; Shah, Mohammad Tahir; Khan, Kifayatullah; Huang, Qing; Ali, Roshan

    2015-01-01

    Several anthropogenic and natural sources are considered as the primary sources of toxic metals in the environment. The current study investigates the level of heavy metals contamination in the flora associated with serpentine soil along the Mafic and Ultramafic rocks northern-Pakistan. Soil and wild native plant species were collected from chromites mining affected areas and analyzed for heavy metals (Cr, Ni, Fe, Mn, Co, Cu and Zn) using atomic absorption spectrometer (AAS-PEA-700). The heavy metal concentrations were significantly (p soil as compared to reference soil, however Cr and Ni exceeded maximum allowable limit (250 and 60 mg kg(-1), respectively) set by SEPA for soil. Inter-metal correlations between soil, roots and shoots showed that the sources of contamination of heavy metals were mainly associated with chromites mining. All the plant species accumulated significantly higher concentrations of heavy metals as compared to reference plant. The open dumping of mine wastes can create serious problems (food crops and drinking water contamination with heavy metals) for local community of the study area. The native wild plant species (Nepeta cataria, Impatiens bicolor royle, Tegetis minuta) growing on mining affected sites may be used for soil reclamation contaminated with heavy metals.

  1. Dihydronepetalactones deter feeding activity by mosquitoes, stable flies, and deer ticks.

    Science.gov (United States)

    Feaster, John E; Scialdone, Mark A; Todd, Robin G; Gonzalez, Yamaira I; Foster, Joseph P; Hallahan, David L

    2009-07-01

    The essential oil of catmint, Nepeta cataria L., contains nepetalactones, that, on hydrogenation, yield the corresponding dihydronepetalactone (DHN) diastereomers. The DHN diastereomer (4R,4aR,7S,7aS)-4,7-dimethylhexahydrocyclopenta[c]pyran-1(3H)-one, DHN 1) was evaluated as mosquito repellent, as was the mixture of diastereomers {mostly (4S,4aR,7S,7aR)-4,7-dimethylhexahydrocyclopenta[c]pyran-1(3H)-one, DHN 2} present after hydrogenation of catmint oil itself. The repellency of these materials to Aedes aegypti L. and Anopheles albimanus Wiedemann mosquitoes was tested in vitro and found to be comparable to that obtained with the well-known insect repellent active ingredient N,N-diethyl-3-methylbenzamide (DEET). DHN 1 and DHN 2 also repelled the stable fly, Stomoxys calcitrans L., in this study. DHN 1, DHN 2, and p-menthane-3,8-diol (PMD), another natural monoterpenoid repellent, gave comparable levels of repellency against An. albimanus and S. calcitrans. Laboratory testing of DHN 1 and DHN 2 using human subjects with An. albimanus mosquitoes was carried out. Both DHN 1 and DHN 2 at 10% (wt:vol) conferred complete protection from bites for significant periods of time (3.5 and 5 h, respectively), with DHN2 conferring protection statistically equivalent to DEET. The DHN 1 and DHN 2 diastereomers were also efficaceous against black-legged tick (Ixodes scapularis Say) nymphs.

  2. The ecological aspect of ethnobotany and ethnopharmacology of population in Bosnia and Herzegovina.

    Science.gov (United States)

    Redzić, Sulejman S

    2007-09-01

    This paper contains first systematical revision of the results on traditional use of wild medicinal and aromatic herbs on the territory of Bosnia and Herzegovina (B&H)--west of Balkan Peninsula; Southeast of Europe. There have been detected 227 plants belonging to 71 different plant families, which are being used with ethno therapeutic purpose. Results were obtained by method of open ethno botanical interview which comprised 150 persons, whose average age was 63. Medicinal plants in ethno therapy are being used either in fresh, raw or dried condition. Different herbal parts, depending on period of vegetation season, sometimes even in winter, are basis for preparation of infusions (59%), decoct (19%), tinctures (4%). Especially original are balms known as Bosnian "mehlems", which are fresh cuted herbal parts mixed with lukewarm resin, raw cow butter or honey. In ethno therapy are mostly being used aerial plant organs. Majority of herbs is being used for treatment of illnesses of respiratory (22%), gastrointestinal (19%) and urinary and genital system (9%), for treatment of skin conditions (11%), as well as for nervous system and heart diseases (16%). The most original plants on the field of ethno pharmacology, comparing with ethno therapy practice of other regions, are as follows: Ballota nigra, Aesculus hippocastanum, Calluna vulgaris, Centaurea cyanus, Euphrasia rostkoviana, Geranium robertianum, Gentiana asclepiadea, Helichrysum italicum, Lycopodium clavatum, Marrubium vulgare, Nepeta cataria, Populus tremula, Ruta graveolens, Tamus communis, Teucrium montanum, T. chamaedrys, and endemic plants Gentiana lutea subsp. symphyandra, Teucrium arduini, Micromeria thymifolia, Satureja montana, S. subspicata, Rhamnus fallax and Viola elegantula. There haven't been noticed significant differences in the frequencies of medicinal plants use among different ethnical groups. But, it has been perceived that longer ethno therapeutic tradition possess inhabitants of sub- and

  3. Isolation and characterization of Burkholderia fungorum Gan-35 with the outstanding ammonia nitrogen-degrading ability from the tailings of rare-earth-element mines in southern Jiangxi, China.

    Science.gov (United States)

    Feng, Ai-Juan; Xiao, Xi; Ye, Cong-Cong; Xu, Xiao-Ming; Zhu, Qing; Yuan, Jian-Ping; Hong, Yue-Hui; Wang, Jiang-Hai

    2017-12-01

    The exploitation of rare-earth-element (REE) mines has resulted in severe ammonia nitrogen pollution and induced hazards to environments and human health. Screening microorganisms with the ammonia nitrogen-degrading ability provides a basis for bioremediation of ammonia nitrogen-polluted environments. In this study, a bacterium with the outstanding ammonia nitrogen-degrading capability was isolated from the tailings of REE mines in southern Jiangxi Province, China. This strain was identified as Burkholderia fungorum Gan-35 according to phenotypic and phylogenetic analyses. The optimal conditions for ammonia-nitrogen degradation by strain Gan-35 were determined as follows: pH value, 7.5; inoculum dose, 10%; incubation time, 44 h; temperature, 30 °C; and C/N ratio, 15:1. Strain Gan-35 degraded 68.6% of ammonia nitrogen under the optimized conditions. Nepeta cataria grew obviously better in the ammonia nitrogen-polluted soil with strain Gan-35 than that without inoculation, and the decrease in ammonia-nitrogen contents of the former was also more obvious than the latter. Besides, strain Gan-35 exhibited the tolerance to high salinities. In summary, strain Gan-35 harbors the ability of both ammonia-nitrogen degradation at high concentrations and promoting plant growth. This work has reported a Burkholderia strain with the ammonia nitrogen-degrading capability for the first time and is also the first study on the isolation of a bacterium with the ammonia nitrogen-degrading ability from the tailings of REE mines. The results are useful for developing an effective method for microbial remediation of the ammonia nitrogen-polluted tailings of REE mines.

  4. El género Calamintha Mill. (Labiatae en la Península Ibérica e Islas Baleares

    Directory of Open Access Journals (Sweden)

    Morales, Ramón

    1997-12-01

    Full Text Available The Iberian species of Calamintha Mill, are studied taxonomically, including chemical, palynological and karyological data. Two species inhabit the geographic área considered: C. nepeta, with two subspecies and C. grandiflora. Each species is accompanied by a distribution map (UTM projection with points equivalent to 10 x 10 km quadrats. The new combinations Calamintha sect. Macromelissa (Benth. R. Morales and C. nepeta subsp. sylvatica (Bromf. R. Morales are proposed.Estudio de las especies ibéricas de Calamintha Mill., teniendo en cuenta datos químicos, palinológicos y cariológicos. En el área geográfica considerada viven dos especies: C. nepeta, con dos subespecies, y C. grandiflora. Se incluyen mapas de distribución. Se proponen las nuevas combinaciones Calamintha sect. Macromelissa (Benth. R. Morales y C. nepeta subsp. sylvatica (Bromf. R. Morales.

  5. Sharpley’s Bottom Historic Sites: Phase I Interdisciplinary Investigations, Tombigbee River Multi-Resource District, Alabama and Mississippi.

    Science.gov (United States)

    1982-01-01

    catnip tea was adopted as a means of treating baby colic . Mrs. Helen Crawford recounts the story of one of her remarkable ancestors, a woman who took...people walked up and down the sidewalks laughing and having a good time. The ice cream parlor was especially busy; Myrtle and friends would sit...outside on the cars eating ice cream and watching the people go by. Inside, the parlor had those iron chairs and tables with the openwork scroll backs

  6. Plant Glandular Trichomes

    Indian Academy of Sciences (India)

    landing on the plant. Glandular trichomes in catmint (Nepeta sp.) produce nepetalactone, closely related to the aphid sex pheromone, nepetalactol. Nepetalactone can be reduced to the corresponding nepetalactol. ... Plant glandular trichomes function either as repositories or releasing sites of various chemicals. Interest in ...

  7. Potentiel antiradicalaire des extraits de feuilles de Bersama ...

    African Journals Online (AJOL)

    BENE KOUADIO

    Catalogue Systématique, Biogéographie et Ecologie. Conservatoire et Jardin. Botanique : Génève,. Switzerland,. Boisiera 57 ; 396. Bougandoura. N,. Bendimerad. N. 2013. Évaluation de l'activité antioxydante des extraits aqueux et méthanolique de Satureja calamintha ssp. Nepeta (L.) Briq. Revue « Nature &. Technologie.

  8. Correction to Hepper and Friis's analysis of Flora Aegyptiaco-Arabica:

    DEFF Research Database (Denmark)

    Ryding, Olof; Friis, Ib

    2014-01-01

    The name Nepeta nepetellae has been accepted in Carl Christensen’s standard index to Forsskål’s Flora Aegyptiaco-Arabica, listed in Index Kewensis and IPNI, listed as an accepted name in The Plant List and accepted in Hepper and Friis’s revision of the plants of Forsskål’s Flora Aegyptiaco-Arabic...... to the currently used names and clarify a complex nomenclatural situation, which has confused several previous authors on the subject. The meanings of asterisked names in Flora Aegyptiaco-Arabica and their bearing for N. nepetellae are briefly reviewed.......The name Nepeta nepetellae has been accepted in Carl Christensen’s standard index to Forsskål’s Flora Aegyptiaco-Arabica, listed in Index Kewensis and IPNI, listed as an accepted name in The Plant List and accepted in Hepper and Friis’s revision of the plants of Forsskål’s Flora Aegyptiaco......-Arabica. If accepted, N. nepetellae may threaten names in current use in Arabia and Africa. However, the name is so similar to the earlier N. nepetella L. that the two names are likely to be confused. Nepeta nepetellae is rejected as a later illegitimate homonym of N. nepetella, which will remove the threat...

  9. Nutrient evaluation and elemental analysis of four medicinal plants of Khyber Pakhtoon Khwa, Pakistan

    International Nuclear Information System (INIS)

    Khan, F.U.; Ullah, R.; Muhammad, Z.; Rehman, N.U.; Zohaib, M.; Shinwari, Z.K.

    2011-01-01

    The study was carried out to assess the nutritional value and mineral contents of four medicinal plants viz., Aerva javanica Burm.f, Calotropis procera Ait. f, Datura alba Nees, and Nepeta suavis Stapf., which are traditionally used as medicine in the Northwest Pakistan. Proximate analysis of plant sample determines that protein (21.353%) and ash (18.803%) was highest in Datura alba, carbohydrate (70.123 %) in Aerva javanica, energy (398.496 Kcal/100g), fats (12.595%) and fibre (40.150%) was highest in Nepeta suavis, while highest moisture (11.255%) was reported in Calotropis procera. In comparative assessment of the various species, the results showed that Nepeta suavis is the most significant species having higher concentrations of fat, fibre and energy values compared to the other species. The essential elements such as Fe, Cd, Cu, Mn, Pb, Cr, Mg and Na have been analysed using Atomic Absorption Spectrometric method from the medicinal plants in variable range. (author)

  10. Aromatic Medicinal Plants of the Lamiaceae Family from Uzbekistan: Ethnopharmacology, Essential Oils Composition, and Biological Activities

    Directory of Open Access Journals (Sweden)

    Nilufar Z. Mamadalieva

    2017-02-01

    Full Text Available Plants of the Lamiaceae family are important ornamental, medicinal, and aromatic plants, many of which produce essential oils that are used in traditional and modern medicine, and in the food, cosmetics, and pharmaceutical industry. Various species of the genera Hyssopus, Leonurus, Mentha, Nepeta, Origanum, Perovskia, Phlomis, Salvia, Scutellaria, and Ziziphora are widespread throughout the world, are the most popular plants in Uzbek traditional remedies, and are often used for the treatment of wounds, gastritis, infections, dermatitis, bronchitis, and inflammation. Extensive studies of the chemical components of these plants have led to the identification of many compounds, as well as essentials oils, with medicinal and other commercial values. The purpose of this review is to provide a critical overview of the literature surrounding the traditional uses, ethnopharmacology, biological activities, and essential oils composition of aromatic plants of the family Lamiaceae, from the Uzbek flora.

  11. Antioxidant activity and cholinesterase inhibition studies of four flavouring herbs from Alentejo.

    Science.gov (United States)

    Arantes, Sílvia; Piçarra, Andreia; Candeias, Fátima; Caldeira, A Teresa; Martins, M Rosário; Teixeira, Dora

    2017-09-01

    Essential oils (EOs) and aqueous extracts of aerial parts of four aromatic species, Calamintha nepeta, Foeniculum vulgare, Mentha spicata and Thymus mastichina, from southwest of Portugal were characterised chemically and analysed in order to evaluate their antioxidant potential and cholinesterase inhibitory activities. The main components of EOs were oxygenated monoterpenes, and aqueous extracts were rich in phenol and flavonoid compounds. EOs and aqueous extracts presented a high antioxidant potential, with ability to protect the lipid substrate, free radical scavenging and iron reducing power. Furthermore, EOs and extracts showed AChE and BChE inhibitory activities higher than rivastigmine, the standard drug. Results suggested the potential use of EOs and aqueous extracts of these flavouring herbs as nutraceutical or pharmaceutical preparations to minimise the oxidative stress and the progression of degenerative diseases.

  12. Enantiomeric Distribution of Some Linalool Containing Essential Oils and Their Biological Activities

    Directory of Open Access Journals (Sweden)

    K. Hüsnü Can Başer

    2010-10-01

    Full Text Available The enantiomeric composition of linalool was determined in 42 essential oils using chiral columns. Essential oils were analyzed by multidimentional gas chromatography-mass spectrometry using a non-chiral and chiral FSC columns combination with modified g -cyclodextrine (Lipodex E as the chiral stationary phase without previous isolation of the compound from the mixture. The essential oils of Achillea, Ballota, Calamintha, Micromeria, Hedychium, Tanacetum, Coriandrum, Xanthoxylum, Ocimum, Thymus, Lavandula, Elettaria, Cinnamomum, Salvia, Origanum, Satureja, Nepeta, Stachys were used as source material for enantiomeric separation of linalool. Enantiomeric distribution of linalool showed (--linalool was much more common than the (+-linalool in the essential oils in this study. (-- and (+-linalool enantiomers were evaluated for antimicrobial, antifungal and antimalarial activities. Both enantiomers demonstrated approximately 50% growth inhibition of Botrytis cinerea at 48 hrs.

  13. EPA-Registered Repellents for Mosquitoes Transmitting Emerging Viral Disease.

    Science.gov (United States)

    Patel, Radha V; Shaeer, Kristy M; Patel, Pooja; Garmaza, Aleksey; Wiangkham, Kornwalee; Franks, Rachel B; Pane, Olivia; Carris, Nicholas W

    2016-12-01

    In many parts of the United States, mosquitoes were previously nuisance pests. However, they now represent a potential threat in the spread of viral diseases. The Aedes aegypti, Aedes albopictus, and Culex species mosquitoes are endemic to the United States and together may transmit a variety of viral diseases of growing concern, including West Nile virus, chikungunya, dengue fever, and Zika virus. The Centers for Disease Control and Prevention and the Environmental Protection Agency (EPA) recommend N,N-diethyl-meta-toluamide (DEET) as a first-line mosquito repellent, but for patients refusing to use DEET or other conventional repellents, guidance is limited to any EPA-registered product. Therefore, we conducted a systematic review of the literature to identify which EPA-registered personal mosquito repellent provides the best protection from A. aegypti, A. albopictus, and Culex spp. mosquitoes. We abstracted data from 62 published reports of EPA-registered mosquito repellents. The conventional repellent picaridin has the strongest data to support its use as a second-line agent, while IR3535 and oil of lemon eucalyptus are reasonably effective natural products. Citronella, catnip, and 2-undecanone offer limited protection or have limited data. These results can be used by pharmacists and other health care professionals to advise patients on the selection of an EPA-registered mosquito repellent. Regardless of the repellent chosen, it is vital for patients to follow all instructions/precautions in the product labeling to ensure safe and effective use. © 2016 Pharmacotherapy Publications, Inc.

  14. Flowers of Çoruh Valley

    Directory of Open Access Journals (Sweden)

    Ramazan Çakmakçı

    2018-01-01

    Full Text Available Coruh valley has an important biological diversity in term of plants, flora-fauna, wildlife and ecosystems. These regions contain the landraces, wild and weedy relatives, other wild, herbaceous and flowering trees, herbaceous flowering plants, medicinal and aromatic and flowering and ornamental shrubs plants species which are especially economically important plant for floriculture, eco-tourism, botanical tourism and nature tourism. Many important medicinal and aromatic and ornamental plants species are found in this region and naturally grow. It is considered that Acantholimon, Achillea, Alkanna, Allium, Amygdalus, Angelica, Anemone, Anthemis, Arabis, Arctium, Artemisia, Asparagus, Asperula, Astragalus, Calamintha, Calendula, Calutea, Campanula, Capparis, Cardamine, Centaurea, Cephalanthera, Cephalaria, Chelidonium, Chenopodium, Chysanthemum, Colchicum, Consolida, Coriandrum, Cornus, Coronilla, Cerasus, Cotoneaster, Crataegus, Crocus, Cyclamen, Dactylorhiza, Digitalis, Dianthus, Draba, Echinops, Equisetum, Ferula, Filipendula, Fritillaria, Fumaria, Gagea, Galanthus, Galium, Genista, Gentiana, Geranium, Geum, Gladiolus, Glychirrza, Helichrysum, Hesperis, Hypericum, İnula, İris, Isatis, Juniperus, Lilium, Linaria, Linum, lysimachia, Malus, Malva, Marrubium, Melissa, Mentha, Micromeria, Morina, Muscari, Mysotis, Narcissus, Neotchichatchewia, Nepeta, Onobrychis, Orchis, Ornithogalum, Origanum, Paeonia, Papaver, Pedicularis, Peganum, Phelypaea, Platanthera, Plantago, Pilosella, Pelargonium, Potentilla, Polygonum, Polygala, Primula, Punica, Prunus, Pyrus, Ranunculus, Rhamnus, Rhododendron, Rhus, Rosa, Rubia, Rubus, Rumex, Salvia, Sambucus, Satureja, Scilla, Scorzonera, Scutellaria, Sedum, Sempervivum, Sideritis, Sophora, Sorbus, Stachys, Tanecetum, Teucrium, Thymus, Trigonella, Tulipa, Tussilago, Uechtriitzia, Vaccinium, Verbascum, Verbena, Veronica, Viburnum and Ziziphora species commonly found in the region may be may be evaluated economically.

  15. New chromosome reports in Lamiaceae of Kashmir (Northwest Himalaya), India.

    Science.gov (United States)

    Malik, Reyaz Ahmad; Gupta, Raghbir Chand; Singh, Vijay; Bala, Santosh; Kumari, Santosh

    2017-03-01

    Meiotic studies and chromosome data are imperative in order to have an overall germplasm evaluation of a taxon. In the present effort, the meiotic study is carried out in 48 populations belonging to 26 species of Lamiaceae collected from their natural habitats in Kashmir Himalaya, which forms an important part of Northwest Himalaya. Chromosome counts in the five species viz. Dracocephalum nutans (2n = 10), Lycopus europaeus (2n = 22), Marrubium vulgare (2n = 54), Nepeta nervosa (2n = 18) and Salvia sclarea (2n = 22) are first time reported from India. Besides, 17 species are cytologically evaluated for the first time from the study area-Kashmir Himalaya. In Marrubium vulgare, hexaploid cytotype (2n = 6 × =54) is reported for the first time. Also, diploid and tetraploid cytomorphovariants are observed in Calamintha vulgaris (2n = 20, 40), Elsholtzia ciliata (2n = 16, 32) and Mentha longifolia (2n = 20, 40). Various meiotic abnormalities like chromatin stickiness, cytomixis, nonsynchronous disjunction, laggards, chromatin bridges, etc. leading to pollen abnormalities have been documented for the first time in some species. The worldwide status of chromosome number data in each genus is presented.

  16. Agricultural, domestic and handicraft folk uses of plants in the Tyrrhenian sector of Basilicata (Italy

    Directory of Open Access Journals (Sweden)

    Guarrera Paolo

    2005-07-01

    Full Text Available Abstract Background Research was carried out into agricultural and domestic-handicraft uses in folk traditions in the Tyrrhenian sector of the Basilicata region (southern Italy, as it is typically representative of ethnobotanical applications in the Mediterranean area. From the point of view of furnishing a botanical support for the study of local "material culture" data was collected through field interviews of 49 informants, most of whom were farmers. Results The taxa cited are 60, belonging to 32 botanical families, of which 18 are employed for agricultural uses and 51 for domestic-handicraft folk uses. Data show a diffuse use of plants for many purposes, both in agricultural (present uses 14%; past uses 1% and for domestic-handicraft use (present uses 40%; past uses 45%; most of the latter are now in decline. Conclusion 60 data look uncommon or typical of the places studied. Some domestic-handicraft folk uses are typical of southern Italy (e.g. the use of Ampelodesmos mauritanicus for making ties, ropes, torches, baskets or that of Acer neapolitanum for several uses. Other uses (e.g. that of Inula viscosa and Calamintha nepeta for peculiar brooms, and of Origanum heracleoticum for dyeing wool red are previously unpublished.

  17. An ethno botanical perspective of traditional medicinal plants from the Khattak tribe of Chonthra Karak, Pakistan.

    Science.gov (United States)

    Rehman, Khalid; Mashwani, Zia-ur-Rehman; Khan, Mubark Ali; Ullah, Zahid; Chaudhary, Hassan Javed

    2015-05-13

    The present study was carried out with an aim to gather, evaluate and analyze the ethno botanical information of medicinal uses of the plant species possessed by the native Khattak tribe of the Chonthra, district Karak Pakistan. The region with poor documentation of traditional knowledge, preserving the local traditional knowledge, reporting new as well as rarely reported medicinal properties of medicinal plants, to be tested experimentally for validation. The medicinal uses of existing plant species were documented by oral communication with 103 people, all over above 60 years of age, born and residing in Chonthra. Information was gathered by semi-structured interviews with further analysis by indices like Relative frequency citation RFC and Medicinal use value MUV. The study resulted with medicinal information on 66 plants species belonging to 34 families (using against 58 health related problems with 83 different preparations mainly administered orally and topical). The dominant families include Brasicaceae and Limiaceae. Withania coagulans and Pegnum harmala were the plant species quoted 100% by the informants with RFC values 1 each. The MUV were scattered between 1.24 and 0.03. The highest MUV were W. coagulans 1.24, Pegnum harmala 1.18, Fagonia cretica 1.14. This study for the first time include Nepeta lagopsis to the ethnobotanical wealth. This study was an extension to the ethnobotanical research conducted in Khyber Pakhtunkhwa (KPK) Pakistan. The target area being small and less number of plants with limited traditional knowledge can serve basis for further work focusing on rarely or non- reported plant species of pharmacological and phytochemical importance with active metabolite capable of broadening the sources of new drugs. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  18. Evaluation of cytotoxic effect of methanolic extracts isolated from endemic plants of Chaharmahal va Bakhtiari province on PC-3, MCF-7, Hep G2, CHO and B16-F10 cell lines

    Directory of Open Access Journals (Sweden)

    Z. Tayarani-Najaran

    2017-11-01

    Full Text Available Background and objectives: To date, thousands of secondary metabolites have been isolated from plants and microorganisms and there is an unprecedented attention towards potential biomedical applications of natural compounds. In this study, cytotoxic properties of methanol extracts of Stachys obtusicrena, Aristolochia olivieri, Linum album, Dionysia sawyeri, Ajuga chamaecistus, Achillea kellalensis, Nepeta glomerulosa, Phlomis aucheria, Tanacetum dumosum, Dianthus orientalis, Scutellaria multicaulis, Cicer oxyodon and Picris oligocephalum which are widely grown in Iran, were investigated on PC-3 (prostat cancer, MCF-7 (breast cancer, Hep-G2 (liver cancer, CHO (ovarian cancer and B16-F10 (melanoma cell lines. Methods: The cancer cells were cultured in RPMI-1640 and incubated with different concentrations of the plant extracts. Cell viability was quantitated by Alamar blue® assay. The apoptotic cells were determined by PI coloring and Flow Cytometry (Sub-G1 peak. Results: The methanol extracts of D. sawyeri, S. obtusicrena, and C. oxyodon significantly decreased the viability of CHO cells. The Methanol extract of D. sawyer and L. album had cytotoxic effects on B16-F10 cells, whereas no toxicity was observed in MCF-7, Hep-G2 and PC-3 cell lines after incubation of the cancer cells with the plant extracts. The PI staining results showed that D. sawyeri, S. obtusicrena, and C. oxyodon in CHO cancer cells could induce apoptosis in a concentration-dependent manner. Conclusion: Screening plants to find the most cytotoxic extract showed D. sawyeri, S. obtusicrena, C. oxyodon and L. album had the potential for further analysis toward finding active phytochemicals with cytotoxic activity.

  19. In vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in Turkey.

    Science.gov (United States)

    Yildirim, Arzu Birinci; Karakas, Fatma Pehlivan; Turker, Arzu Ucar

    2013-08-01

    To investigate antibacterial and antitumor activities of 51 different extracts prepared with 3 types of solvents (water, ethanol and methanol) of 16 different plant species (Ajuga reptans (A. reptans) L., Phlomis pungens (P. pungens) Willd., Marrubium astracanicum (M. astracanicum) Jacq., Nepeta nuda (N. nuda) L., Stachys annua (S. annua) L., Genista lydia (G. lydia) Boiss., Nuphar lutea (N. lutea) L., Nymphaea alba (N. alba) L., Vinca minor (V. minor) L., Stellaria media (S. media) L., Capsella bursa-pastoris (C. bursa-pastoris) L., Galium spurium (G. spurium) L., Onosma heterophyllum (O. heterophyllum) Griseb., Reseda luteola (R. luteola) L., Viburnum lantana (V. lantana) L. and Mercurialis annua (M. annua) L.) grown in Turkey was conducted. Antibacterial activity was evaluated with 10 bacteria including Streptococcus pyogenes (S. pyogenes), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Escheria coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Salmonella typhimurium (S. typhimurium), Serratia marcescens (S. marcescens), Proteus vulgaris (P. vulgaris), Enterobacter cloacae (E. cloacea), and Klebsiella pneumoniae (K. pneumoniae) by using disc diffusion method. Antitumor activity was evaluated with Agrobacterium tumefaciens (A. tumefaciens)-induced potato disc tumor assay. Best antibacterial activity was obtained with ethanolic extract of P. pungens against S. pyogenes. Ethanolic and methanolic extract of N. alba and ethanolic extract of G. lydia also showed strong antibacterial activities. Results indicated that alcoholic extracts especially ethanolic extracts exhibited strong antibacterial activity against both gram-positive and gram-negative bacteria. Best antitumor activity was obtained with methanolic extracts of N. alba and V. lantana (100% tumor inhibition). Ethanolic extract of N. alba, alcoholic extracts of N. lutea, A. reptans and V. minor flowers, methanolic extracts of G. lydia and O. heterophyllum and ethanolic

  20. Investigation of nepetolide as a novel lead compound: Antioxidant, antimicrobial, cytotoxic, anticancer, anti-inflammatory, analgesic activities and molecular docking evaluation

    Directory of Open Access Journals (Sweden)

    Tanzeel ur Rehman

    2018-03-01

    Full Text Available In the present study, we describe various pharmacological effects and computational analysis of nepetolide, a tricyclic clerodane-type diterpene, isolated from Nepeta suavis. Nepetolide concentration-dependently (1.0–1000 µg/mL exhibited 1,1-diphenyl,2-picrylhydrazyl free radical scavenging activity with maximum effect of 87.01 ± 1.85%, indicating its antioxidant potential, as shown by standard drug, ascorbic acid. It was moderately active against bacterial strain of Staphylococcus aureus. In brine shrimp’s lethality model, nepetolide potently showed cytotoxic effect, with LC50 value of 8.7 µg/mL. When evaluated for antitumor activity in potato disc tumor assay, nepetolide exerted tumor inhibitory effect of 56.5 ± 1.5% at maximum tested concentration of 1000 µg/mL. Nepetolide at 20 mg/kg reduced carrageenan-induced inflammation (P < .001 vs. saline group in rat paw. Nepetolide dose-dependently (100–500 mg/kg decreased acetic acid evoked writhes, as exhibited by diclofenac sodium. In-silico investigation of nepetolide was carried out against cyclooxygenase-2, epidermal growth factor receptor and lipoxygenase-2 targets. Virtual screening through Patchdock online docking server identified primarily hydrophobic interactions between ligand nepetolide and receptors proteins. Enhanced hydrogen bonding was predicted with Autodock showing 6–8 hydrogen bonds per target. These results indicate that nepetolide exhibits antioxidant, antibacterial, cytotoxic, anticancer, anti-inflammatory and analgesic activities and should be considered as a lead compound for developing drugs for the remedy of oxidative stress-induced disorders, microbial infections, cancers, inflammations and pain.

  1. Ethnoveterinary medicines used for ruminants in British Columbia, Canada

    Science.gov (United States)

    Lans, Cheryl; Turner, Nancy; Khan, Tonya; Brauer, Gerhard; Boepple, Willi

    2007-01-01

    helix and Alchemilla vulgaris are fed for retained placenta. Conclusion Some of the plants showing high levels of validity were Hedera helix for retained placenta and Euphrasia officinalis for eye problems. Plants with high validity for wounds and injuries included Hypericum perforatum, Malva parviflora and Prunella vulgaris. Treatments with high validity against endoparasites included those with Juniperus communis and Pinus ponderosa. Anxiety and pain are well treated with Melissa officinalis and Nepeta caesarea. PMID:17324258

  2. Ethnomedicinal uses of plants in the treatment of paediatric geohelminth infections in Kalat district of Northern Balochistan, Pakistan.

    Science.gov (United States)

    Bibi, Tahira; Ahmad, Mushtaq; Edwards, Sarah E; Tareen, Niaz Mohammad; Jabeen, Rukhsana; Abdullah, Irum

    2016-05-13

    Infection by intestinal parasitic worms (soil-transmitted helminths or geohelminths) is prevalent in many parts of the world, and poses a particular health risk to children. This paper presents findings from a preliminary study with the primary aim to document indigenous knowledge about the use of herbal medicines in the treatment of intestinal worm infections in children among the local communities of Kalat district of northern Balochistan, Pakistan. Ethnomedicinal data were collected through a triangulation approach, that included participant-observation and rapid appraisal methods. Prior-informed consent (PIC) was obtained from participants before conducting structured and semi-structured interviews and delivering an open ended questionnaire. A total of 94 participants, including 28 men (of whom 7 were traditional healers), and 66 women of four different age groups were interviewed. Results were analyzed using quantitative indices of Use Value citations (UVC) and Disease-Consensus Index (DCI). Fewer men than women agreed to be interviewed, thus overall women in the area appeared to have more ethnomedicinal knowledge. The majority of study participants belonged to the older age group (>55 years). A total of 49 plant species, belonging to 47 genera, distributed in 30 families were reported. The families Asteraceae and Lamiaceae were most frequently represented, with four species each. Trees were the most common life form, with seeds the most frequently cited plant part used (29%). Nearly a third (31%) of plant-based remedies reported in the treatment of intestinal worms were administered as a decoction. The highest UVC and DCI was reported for the species Ferula assa-foetida sL. (UVC 0.51, DCI 0.46). This study provides previously unreported data on the use of medicinal plants in the treatment of geohelminth infections in children of Kalat. Eight species, Acacia modesta Wall., Asparagus capitatus Baker, Microcephala lamellata (Bunge) Pobed., Nepeta praetervisa

  3. Ethnoveterinary medicines used for ruminants in British Columbia, Canada

    Directory of Open Access Journals (Sweden)

    Brauer Gerhard

    2007-02-01

    Symphytum officinale. Hedera helix and Alchemilla vulgaris are fed for retained placenta. Conclusion Some of the plants showing high levels of validity were Hedera helix for retained placenta and Euphrasia officinalis for eye problems. Plants with high validity for wounds and injuries included Hypericum perforatum, Malva parviflora and Prunella vulgaris. Treatments with high validity against endoparasites included those with Juniperus communis and Pinus ponderosa. Anxiety and pain are well treated with Melissa officinalis and Nepeta caesarea.

  4. Ethnobotanical study on medicinal plants in villages of Çatak (Van-Turkey).

    Science.gov (United States)

    Mükemre, Muzaffer; Behçet, Lütfi; Çakılcıoğlu, Uğur

    2015-05-26

    This paper provides significant ethnobotanical information on medicinal plants in the villages of Çatak in the Eastern Anatolia Region. Recording such data calls for urgency. This is the first ethnobotanical study in which statistical calculations about plants are carried out by means of FIC method in Eastern (Van) part of Turkey. This study aims to identify the wild plants collected for medicinal purposes by locals of Çatak which is located in the Eastern Anatolia Region of Turkey, and to identify the uses and local names of these wild plants. A field study had been carried out for a period of approximately 2 years (2010-2012). During this period, 78 plants taxa were collected. Demographic characteristics of participants, names of the local plants, their utilized parts and preparation methods were investigated and recorded. The plant taxa were collected within the scope of the study; and herbarium materials were prepared. In addition, the relative significance value of the taxa was determined, and informant consensus factor (FIC) was calculated for the medicinal plants included in the study. We have found out in the literature review of the plants included in our study that 78 plant taxa are already used for medicinal purposes while 19 plants are not available among the records in the literature. The most common families are Asteraceae, Apiaceae, Lamiaceae, Rosaceae, Euphorbiaceae, Fabaceae, and Malvaceae. We include in our study and report for the first time the medicinal uses of Alchemilla buseriana Rothm., Astragalus longifolius Lam., Cephalaria microcephala Boiss., Euphorbia grisophylla M.S. Khan, Fritillaria crassifolia Boiss. & Huet. subsp. kurdica (Boiss. & Noe) Rix, Fritillaria pinardii Boiss., Malabaila lasiocarpa Boiss., Nepeta betonicifolia C.A. Mey., Onobrychis altissima Grossh., Onobrychis carduchorum C.C. Townsend, Papaver bracteatum Lindl., Phlomis tuberosa L., Psephellus karduchorum (Boiss.) Wagenitz, Scutellaria orientalis L. subsp. pichleri