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Sample records for catfish rhamdia quelen

  1. Pyrethroid toxicity in silver catfish, Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    Francisco P. Montanha

    2012-12-01

    Full Text Available This study aimed to determine both the lethal and sublethal concentrations of Cypermethrin in young Silver Catfish (Brazilian "Jundiá", Rhamdia quelen on aquatic environment during 96 hours, as well as to determine the Cypermethrin and Deltamethrin sublethal concentrations during the initial embryonic development period of Rhamdia quelen, and to verify their respective rates of fertilization, hatching and survival. Pyrethroid nowadays is a widely used insecticide, which presents a high toxicity to fish. In order to determine lethal and sublethal concentrations, 120 silver catfish were used; each one had an average weight of 59.58±4.50g and an average size of 20.33±2.34cm. Concentrations used were 0, 1.0, 1.5, 2.0, 2.5, 3.0, 5.0, 10.0, 15.0 and 20.0mg of Cypermethrin per liter of water (mg/L. Fish were exposed to the product in 30-liter fish tanks. In each fish tank there were four fishes and the product was applied three times, i.e., a total of twelve fish were exposed to the product at each application, and a total of 120 fish during the entire experiment (n=120. In order to determine the Cypermethrin and Deltamethrin sublethal concentrations during the initial embryonic development, ovulation induction was performed on female fishes using hormones, and then and egg collection was performed. The eggs were then hydrated and fertilized in Cypermethrin and Deltamethrin in different concentrations: 0.001, 0.01, 0.1, 1.0 and 10.0mg/L of Cypermethrin and 0.001, 0.01, 0.1, 0.5 and 1.0mg/L of Deltamethrin, in addition to the control group (0mg/L. After fertilization, the eggs were kept in containers with the respective pesticides of Cypermethrin and Deltamethrin until hatching, when hatching rate was verified. Then the alevins, from the hatching, were kept on their respective concentrations of Cypermethrin and Deltamethrin so that the survival rate could be analyzed regarding the tested insecticides, during both 12-hour and 24-hour periods

  2. Pyrethroid toxicity in silver catfish, Rhamdia quelen Toxicidade dos piretróides sobre Jundiás, Rhamdia quelen

    OpenAIRE

    Montanha, Francisco P.; Luciana A.G. Galeb; Jorge D. Mikos; Luciana N. Ganeco; Tiago P. Pereira; Anne Tanaka; Peter G. Kirschnik; Cláudia T. Pimpão

    2012-01-01

    This study aimed to determine both the lethal and sublethal concentrations of Cypermethrin in young Silver Catfish (Brazilian "Jundiá", Rhamdia quelen) on aquatic environment during 96 hours, as well as to determine the Cypermethrin and Deltamethrin sublethal concentrations during the initial embryonic development period of Rhamdia quelen, and to verify their respective rates of fertilization, hatching and survival. Pyrethroid nowadays is a widely used insecticide, which presents a high toxic...

  3. Salinity on artificial reproduction of silver catfish (Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    Gabriel Bernardes Martins

    2015-03-01

    Full Text Available Attempting to improve reproduction performance and ichthyo prophylaxis, this study evaluated the effects of maintaining silver catfish (Rhamdia quelen broodstock in different saline concentrations (0, 2, 4, 6 and 8‰ on gametes quality and reproductive viability. The results showed that sperm percent motility did not change between 0 and 4‰, but it was reduced at 6‰, and sperm became immotile at 8‰ salinity. Sperm motility time was increased (almost five fold at 6‰. Salinities up to 4‰ prevented fertilization and hatching, proving their deleterious effects on oocytes and embryos. Therefore, media up to 4‰ salinity may be an alternative for icthyo prophylaxis, although fertilization and incubation must be done in freshwater medium

  4. Celioscopic liver biopsy in silver catfish (Rhamdia quelen

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    João P.S. Feranti

    2015-01-01

    Full Text Available Endosurgery has been used for assessment of fish celomatic cavity, as well as for obtaining biopsies for organic analysis. Such minimally invasive access may also be used for the analysis of environmental impact on biomarkers of pollution. In Brazil, studies and literature regarding the use of celioscopy in fish are sparse. The purpose of the current study was to develop a two-port celioscopy technique to obtain liver biopsy in silver catfish (Rhamdia quelen. Six adult female silver catfish were used. The animals were anesthetized and the inspection of the celomatic cavity were performed using a telescope and celioscopic-guided liver biopsy were taken using laparoscopic Kelly forceps. On the early postoperative period, the animals were released in a confined water reservoir where mortality could be checked. The liver samples were sent for histological assessment. There were no complications during surgery on early postoperative period. It was possible to visualize meticulously several organs (liver, spleen, stomach, pancreas, swim bladder, ovaries, bowel and transverse septum. In conclusion, the surgical technique and the anesthetic protocol proposed were suitable to perform liver biopsies in silver catfish and provided low morbidity.

  5. Survival and behavior of silver catfish, Rhamdia quelen, submitted to antibiotics and sodium chloride treatments

    OpenAIRE

    Andrade Luciana Segura de; Andrade Rafael Luiz Barboza de; Becker Alexssandro Geferson; Baldisserotto Bernardo

    2006-01-01

    In order to evaluate the use of antibiotics and NaCl on the behavior and survival of silver catfish, Rhamdia quelen, infested by Ichthyophthirius multifiliis and infected with Aeromonas hydrophila, juveniles were treated with chloramphenicol, chloramphenicol + salt, oxytetracycline, oxytetracycline + salt and water alone (control). Fish survival in the treatments with chloramphenicol + salt and oxytetracycline + salt was significantly higher than in the other treatments. The treatment with ch...

  6. Pyrethroid toxicity in silver catfish, Rhamdia quelen Toxicidade dos piretróides sobre Jundiás, Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    Francisco P. Montanha

    2012-12-01

    Full Text Available This study aimed to determine both the lethal and sublethal concentrations of Cypermethrin in young Silver Catfish (Brazilian "Jundiá", Rhamdia quelen on aquatic environment during 96 hours, as well as to determine the Cypermethrin and Deltamethrin sublethal concentrations during the initial embryonic development period of Rhamdia quelen, and to verify their respective rates of fertilization, hatching and survival. Pyrethroid nowadays is a widely used insecticide, which presents a high toxicity to fish. In order to determine lethal and sublethal concentrations, 120 silver catfish were used; each one had an average weight of 59.58±4.50g and an average size of 20.33±2.34cm. Concentrations used were 0, 1.0, 1.5, 2.0, 2.5, 3.0, 5.0, 10.0, 15.0 and 20.0mg of Cypermethrin per liter of water (mg/L. Fish were exposed to the product in 30-liter fish tanks. In each fish tank there were four fishes and the product was applied three times, i.e., a total of twelve fish were exposed to the product at each application, and a total of 120 fish during the entire experiment (n=120. In order to determine the Cypermethrin and Deltamethrin sublethal concentrations during the initial embryonic development, ovulation induction was performed on female fishes using hormones, and then and egg collection was performed. The eggs were then hydrated and fertilized in Cypermethrin and Deltamethrin in different concentrations: 0.001, 0.01, 0.1, 1.0 and 10.0mg/L of Cypermethrin and 0.001, 0.01, 0.1, 0.5 and 1.0mg/L of Deltamethrin, in addition to the control group (0mg/L. After fertilization, the eggs were kept in containers with the respective pesticides of Cypermethrin and Deltamethrin until hatching, when hatching rate was verified. Then the alevins, from the hatching, were kept on their respective concentrations of Cypermethrin and Deltamethrin so that the survival rate could be analyzed regarding the tested insecticides, during both 12-hour and 24-hour periods

  7. The Ultrastructure of Secretory Cells of the Islets of Langerhans in South American Catfish Rhamdia quelen

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    Laura Luchini

    2015-01-01

    Full Text Available The present work shows that a detailed description of the ultrastructure of the secretory cells of the South American catfish Rhamdia quelen pancreatic islets is presented. Evidence is offered to support the contention that the α-granules consist of a central and an outer portion of different electron densities and solubilities, that the δ-cells are most probably morphologically altered but viable α-cells, and that the β-granules possibly possess a repeating substructure and may therefore represent an intracellular crystalline storage form of insulin.

  8. Survival and behavior of silver catfish, Rhamdia quelen, submitted to antibiotics and sodium chloride treatments

    Directory of Open Access Journals (Sweden)

    Andrade Luciana Segura de

    2006-01-01

    Full Text Available In order to evaluate the use of antibiotics and NaCl on the behavior and survival of silver catfish, Rhamdia quelen, infested by Ichthyophthirius multifiliis and infected with Aeromonas hydrophila, juveniles were treated with chloramphenicol, chloramphenicol + salt, oxytetracycline, oxytetracycline + salt and water alone (control. Fish survival in the treatments with chloramphenicol + salt and oxytetracycline + salt was significantly higher than in the other treatments. The treatment with chloramphenicol presented higher survival than the treatment with oxytetracycline and both showed significantly higher survival than control. Swimming activity was higher in the fish treated with antibiotics and salt compared to control fish. A combination of the studied antibiotics plus salt is more effective to treat both A. hydrophila infection and I. multifiliis infestation in silver catfish, but since the use of chloramphenicol is not allowed in Brazil, oxytetracycline plus salt seems to be the best treatment option.

  9. Rendimento e viabilidade da extração de hipófise de jundiá (Rhamdia quelen Silver catfish (Rhamdia quelen pituitary yield and viability

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    Carine de Vargas Colpo

    2011-05-01

    Full Text Available O extrato hipofisário é provavelmente o indutor hormonal para desova e espermiação mais utilizado em piscicultura. A possibilidade de o produtor ter uma nova fonte de renda motivou a execução deste trabalho. O objetivo foi verificar o rendimento da hipófise no jundiá (Rhamdia quelen, em função do peso, e analisar a viabilidade econômica de sua extração. Exemplares (n=116 com pesos entre 158-1.543g foram abatidos para extração da hipófise. O índice gonadossomático (IGS também foi calculado para verificar sua relação com o peso da hipófise. A hipófise apresentou relação significativa com o peso dos jundiás, mas não com o IGS. A extração da hipófise é mais uma opção de renda para a cadeia produtiva dessa espécie.The pituitary extract is probably the most used hormonal inducer to spawning and spermiation in fish culture. The possibility of a new income source for the fish farmer was the reason of this study. The aim of the present study was to verify the yield of the pituitary in silver catfish (Rhamdia quelen as a function of weight and to analyze the economical viability of its extraction. Specimens (n=116 with 158-1543g were sacrificed for pituitary extraction. The gonadosomatic index (GSI was also calculated to verify its relationship with the pituitary weight. The pituitary showed a significant relationship with silver catfish weight, but not with the GSI. The extraction of the pituitary is another profitable option to silver catfish productive market.

  10. Effects of clomazone herbicide on hematological and some parameters of protein and carbohydrate metabolism of silver catfish Rhamdia quelen.

    Science.gov (United States)

    Crestani, Márcia; Menezes, Charlene; Glusczak, Lissandra; Dos Santos Miron, Denise; Lazzari, Rafael; Duarte, Marta F; Morsch, Vera Maria; Pippi, Amy Lee; Vieira, Vânia Pimentel

    2006-09-01

    The effects of clomazone (0.5 and 1.0 mg/L) according to nominal concentrations used in paddy rice fields (0.4-0.7 mg/L) on protein and carbohydrate metabolism and haematological parameters were evaluated in silver catfish (Rhamdia quelen) after 12, 24, 48, 96 and 192 h of exposure with a recovery period of 96 and 192 h. Liver glycogen increased significantly (Pclomazone concentrations (Pclomazone exposure (22-67%), but reduced in the liver (PClomazone concentrations used in this study appear safe to fish, Rhamdia quelen, because overall parameters can be recovered after 96 and 192 h in clean water. ALT and AST activity may be an early biomarker of clomazone toxicity.

  11. Anesthetic activity of Brazilian native plants in silver catfish (Rhamdia quelen

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    Lenise de Lima Silva

    2013-06-01

    Full Text Available There is an increasing demand for inexpensive and safe anesthetics that can reduce fish stress caused by some procedures such as capture and handling. In this context, the present study evaluated the potential of essential oils (EO of three Brazilian native plants (Hesperozygis ringens, Lippia sidoides and Ocotea acutifolia as anesthetics for the silver catfish - Rhamdia quelen. Moreover, an analysis was made of the chemical composition of these oils and their influence on stress parameter. EO of H. ringens and O. acutifolia were effective as anesthetics, without behavioral side effects. EO of O. acutifolia (150 µL L-1 promoted an increase in blood glucose level. Regarding to the composition, pulegone accounts for 96.63% of the EO of H. ringens, and caryophyllene oxide amounts to 56.90% of the EO of O. acutifolia. Two chemotypes, thymol and carvacrol (68.40% and 67.89%, respectively were verified for EO of L. sidoides. Both samples of EO of L. sidoides showed anesthetic activity in silver catfish, but exposure also caused loss of mucus and mortality. Thus, only the EO of H. ringens and O. acutifolia are advised for anesthetic use

  12. Transgene transmission in South American catfish (Rhamdia quelen) larvae by sperm-mediated gene transfer

    Indian Academy of Sciences (India)

    Tiago Collares; Vinicius Farias Campos; Fabiana Kömmling Seixas; Paulo V Cavalcanti; Odir A Dellagostin; Heden Luiz M Moreira; João Carlos Deschamps

    2010-03-01

    The silver catfish (Rhamdia quelen) is an endemic American fish species. The sperm of each species has its own peculiarities and biological characteristics, which influence the success of mass DNA transfer methods. Our objective in this study was to evaluate different sperm-mediated gene transfer (SMGT) methods to obtain transgenic silver catfish. Different treatments for the incorporation of a foreign pEGFP plasmid group were used: (1) dehydrated/rehydrated (DR), (2) dehydrated/rehydrated/electroporated (DRE), (3) electroporated (E), (4) incubated with seminal plasma (INC); and (5) incubated in the absence of seminal plasma (INCSP). Sperm motility, time of activity duration (TAD), fertilization rate (FR), hatching rate (HR) and sperm morphology were also evaluated. The polymerase chain reaction (PCR) positivity rates for the presence of the transgene were: DRE 60%; DR 40%; E 25%; INC 5% and INCSP 25%. The rates of embryo EGFP expression were: DRE 63%; DR 44%; E 34%; INC 8% and INCSP 38%. The fertilization rate in the control and DRE treatments groups were higher than in the DR group, but the E, INC and INCSP treatment groups had the lowest rate. The hatching rates of the DRE, DR and control groups were higher than in the INCSP, INC and E treatment groups ( > 0.05). There were no differences among the DRE and DR, E and DR, E and INCSP groups in expression and PCR positivity rates of enhanced green fluorescent protein (EGFP) in embryos. Scanning electron microscopy also did not show any change in sperm morphology among treatment groups. To the best of our knowledge, this is the first report on transgene transmission of exogenous DNA into silver catfish larvae through SMGT technology

  13. Inactivated Parapoxvirus ovis as inducer of immunity in silver catfish (Rhamdia quelen

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    TATIANA R. PAVAN

    2016-01-01

    Full Text Available ABSTRACT Molecules with immune modulating activity are ubiquitously distributed in nature and their impact on aquaculture has been exploited in order to increase fish resistance to pathogens. Here, we investigated the effect of inactivated Parapoxvirus ovis (iPPVO on blood cells and innate and acquired immune response of silver catfish (Rhamdia quelen. iPPVO inoculation had no effect on respiratory burst activity; however, following iPPVO inoculation, we observed a significant decrease on circulating monocytes concomitantly with an increased number of heterophilic granulocytes and thrombocytes, which are the main cells involved in innate immunity and provide connection with acquired immunity. Fish inoculated with a combination of bovine serum albumin (BSA + iPPVO had significantly higher levels of antibodies to BSA compared to fish inoculated with BSA alone, but lower than fish inoculated with BSA + Freund's incomplete adjuvant (FIA. These findings points to the potential usefulness of iPPVO as immunomodulator in fish and instigate further research to identify its component that interact with immune cells and that could be exploited as adjuvants in fish.

  14. Biochemistry, cytogenetics and bioaccumulation in silver catfish (Rhamdia quelen) exposed to different thorium concentrations

    International Nuclear Information System (INIS)

    The objective of this study was to evaluate the effect of thorium (Th) bioaccumulation on the metabolism of silver catfish (Rhamdia quelen) through biochemical parameters of the muscle (glycogen, glucose, lactate, protein, and ammonia). In addition, lipidic peroxidation levels (TBARS), catalase (CAT) and glutathione-S-transferase (GST) in the gills and in hepatic and muscular tissues were also analyzed. Cytogenetic parameters were studied through the evaluation of nuclear abnormalities in red blood cells. Silver catfish juveniles were exposed to different waterborne Th levels (in μg L-1): 0 (control), 25.3 ± 3.2, 69.2 ± 2.73, 209.5 ± 17.6, and 608.7 ± 61.1 for 15 days. The organs that accumulated the highest Th levels were the gills and skin. The increase of waterborne Th concentration corresponded to a progressive increase of Th levels in the gills, liver, skin and kidneys, with the highest accumulation in the gills and skin. Metabolic intermediates in the muscle were altered by Th exposure, but no clear relationship was found. CAT and GST activities in the hepatic and muscular tissues of this species suggest that the enzymatic activities can be stimulated at the lowest Th levels and inhibited at the higher levels (mainly in 608.7 μg L-1). The results of the cytogenetic assay contribute to this hypothesis because the higher toxicity in blood samples was found in juveniles exposed to 69.2 and 209.5 μg L-1 Th

  15. Biochemistry, cytogenetics and bioaccumulation in silver catfish (Rhamdia quelen) exposed to different thorium concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Correa, Lizelia M.; Kochhann, Daiani; Becker, Alexssandro G.; Pavanato, Maria A. [Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil); Llesuy, Susana F. [Departamento de Quimica Analitica y Fisicoquimica, Universidad de Buenos Aires, Buenos Aires (Argentina); Loro, Vania L.; Raabe, Alice [Departamento de Quimica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil); Mesko, Marcia F. [Departamento de Quimica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil); Polo em Cruz Alta, Universidade Estadual do Rio Grande do Sul, 98025-810 Cruz Alta, RS (Brazil); Flores, Erico M.M.; Dressler, Valderi L. [Departamento de Quimica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil); Baldisserotto, Bernardo [Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil)], E-mail: bernardo@smail.ufsm.br

    2008-07-30

    The objective of this study was to evaluate the effect of thorium (Th) bioaccumulation on the metabolism of silver catfish (Rhamdia quelen) through biochemical parameters of the muscle (glycogen, glucose, lactate, protein, and ammonia). In addition, lipidic peroxidation levels (TBARS), catalase (CAT) and glutathione-S-transferase (GST) in the gills and in hepatic and muscular tissues were also analyzed. Cytogenetic parameters were studied through the evaluation of nuclear abnormalities in red blood cells. Silver catfish juveniles were exposed to different waterborne Th levels (in {mu}g L{sup -1}): 0 (control), 25.3 {+-} 3.2, 69.2 {+-} 2.73, 209.5 {+-} 17.6, and 608.7 {+-} 61.1 for 15 days. The organs that accumulated the highest Th levels were the gills and skin. The increase of waterborne Th concentration corresponded to a progressive increase of Th levels in the gills, liver, skin and kidneys, with the highest accumulation in the gills and skin. Metabolic intermediates in the muscle were altered by Th exposure, but no clear relationship was found. CAT and GST activities in the hepatic and muscular tissues of this species suggest that the enzymatic activities can be stimulated at the lowest Th levels and inhibited at the higher levels (mainly in 608.7 {mu}g L{sup -1}). The results of the cytogenetic assay contribute to this hypothesis because the higher toxicity in blood samples was found in juveniles exposed to 69.2 and 209.5 {mu}g L{sup -1} Th.

  16. The use of eugenol against Aeromonas hydrophila and its effect on hematological and immunological parameters in silver catfish (Rhamdia quelen).

    Science.gov (United States)

    Sutili, Fernando Jonas; Kreutz, Luiz Carlos; Noro, Mirela; Gressler, Leticia Trevisan; Heinzmann, Berta Maria; de Vargas, Agueda Castagna; Baldisserotto, Bernardo

    2014-02-15

    The aim of this study was to evaluate the activity of eugenol against the fish pathogen Aeromonas hydrophila and eugenol's effect on hematological and natural immune parameters in silver catfish (Rhamdia quelen). In vitro, eugenol showed weak activity against A. hydrophila, but in vivo, at a subinhibitory concentration (10 mg L(-1)), it promoted survival in infected silver catfish. Eugenol (50 μg mL(-1)) reduced the hemolytic activity of A. hydrophila supernatant in vitro in fish erythrocytes. Subjecting catfish to eugenol baths (5 and 10 mg L(-1)) for five days did not alter the hematological and immunological parameters studied in this work. Based on these results, eugenol can be used to treat or prevent bacterial diseases in fish.

  17. Increased immunoglobulin production in silver catfish (Rhamdia quelen exposed to agrichemicals

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    L.C. Kreutz

    2014-06-01

    Full Text Available Fish vaccination has been increasingly exploited as a tool to control pathogen infection. The production of immunoglobulin following vaccination might be affected by several factors such as management procedures, water temperature, and the presence of xenobiotics. In the present study, we aimed to investigate the kinetics of immunoglobulin production in silver catfish (Rhamdia quelen inoculated with inactivated Aeromonas hydrophila and kept at two different water temperatures (17.4±0.4° or 21.3±0.3°C. The effect of a second antigen inoculation and exposure of fish to sublethal concentrations of the herbicides atrazine and glyphosate at 10% of the lethal concentration (LC50-96h on specific serum antibodies were also investigated. Antibodies to A. hydrophila were detected as early as 7 days post-inoculation and increased steadily up to 35 days. The kinetics of antibody production were similar in fish kept at 17.4±0.4° and 21.3±0.3°C, and reinoculation of antigen at 21 days after priming failed to increase specific antibody levels. Intriguingly, we found that, in fish exposed to atrazine and glyphosate, the secretion of specific antibodies was higher than in non-exposed inoculated fish. These findings are important for the design of vaccines and vaccination strategies in Neotropical fish species. However, because atrazine and glyphosate are widespread contaminants of soil and water, their immune-stimulating effect could be harmful, in that fish living in herbicide-contaminated water might have increased concentrations of nonspecific antibodies that could mediate tissue injury.

  18. Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes.

    Science.gov (United States)

    Silva, M A; Costa, G M J; Lacerda, S M S N; Brandão-Dias, P F P; Kalapothakis, E; Silva Júnior, A F; Alvarenga, E R; França, L R

    2016-05-01

    Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of functional sperm and healthy progeny approximately 2months after allogeneic transplantation. In the present study, we evaluated the viability of the adult Nile tilapia testis to generate sperm after xenogeneic transplant of germ cells from sexually mature Jundia catfish (Rhamdia quelen) that belong to a different taxonomic order. Therefore, in order to investigate at different time-periods post-transplantation, the presence and development of donor PKH26 labeled catfish germ cells were followed in the tilapia seminiferous tubules. From 7 to 20days post-transplantation, only PKH26 labeled spermatogonia were observed, whereas spermatocytes at different stages of development were found at 70days. Germ cell transplantation success and progression of spermatogenesis were indicated by the presence of labeled PKH26 spermatids and sperm on days 90 and 120 post-transplantation, respectively. Confirming the presence of the catfish genetic material in the tilapia testis, all recipient tilapias evaluated (n=8) showed the genetic markers evaluated. Therefore, we demonstrated for the first time that the adult Nile tilapia testis offers the functional conditions for development of spermatogenesis with sperm production from a fish species belonging to a different order, which provides an important new venue for aquaculture advancement.

  19. Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes.

    Science.gov (United States)

    Silva, M A; Costa, G M J; Lacerda, S M S N; Brandão-Dias, P F P; Kalapothakis, E; Silva Júnior, A F; Alvarenga, E R; França, L R

    2016-05-01

    Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of functional sperm and healthy progeny approximately 2months after allogeneic transplantation. In the present study, we evaluated the viability of the adult Nile tilapia testis to generate sperm after xenogeneic transplant of germ cells from sexually mature Jundia catfish (Rhamdia quelen) that belong to a different taxonomic order. Therefore, in order to investigate at different time-periods post-transplantation, the presence and development of donor PKH26 labeled catfish germ cells were followed in the tilapia seminiferous tubules. From 7 to 20days post-transplantation, only PKH26 labeled spermatogonia were observed, whereas spermatocytes at different stages of development were found at 70days. Germ cell transplantation success and progression of spermatogenesis were indicated by the presence of labeled PKH26 spermatids and sperm on days 90 and 120 post-transplantation, respectively. Confirming the presence of the catfish genetic material in the tilapia testis, all recipient tilapias evaluated (n=8) showed the genetic markers evaluated. Therefore, we demonstrated for the first time that the adult Nile tilapia testis offers the functional conditions for development of spermatogenesis with sperm production from a fish species belonging to a different order, which provides an important new venue for aquaculture advancement. PMID:26972155

  20. Integrated assessment of biomarker response in carp (Cyprinus carpio) and silver catfish (Rhamdia quelen) exposed to clomazone.

    Science.gov (United States)

    Murussi, Camila R; Costa, Maiara; Menezes, Charlene; Leitemperger, Jossiele; Guerra, Luciana; Lópes, Thais; Severo, Eduardo; Zanella, Renato; Loro, Vania Lucia

    2015-05-01

    Clomazone is considered a potential contaminant of groundwater and is persistent in the environment. To verify the effects of clomazone in Cyprinus carpio and Rhamdia quelen, a method that combines biomarker responses into an index of "integrated biomarker response" (IBR) was used for observed biological alterations in these species. Thiobarbituric acid-reactive substances in liver of carp and silver catfish decreased at both concentrations tested. However, in muscle it increased in carp at 3 mg/L and silver catfish at 6 mg/L. Protein carbonyl increased in liver (3 and 6 mg/L) and muscle (6 mg/L) of carp. In carp, superoxide dismutase (SOD) increased at 3 mg/L and catalase at 6 mg/L. In silver catfish, SOD in liver decreased at 3 mg/L. Glutathione-S-transferase increased at 3 mg/L in muscle of carp. Nonprotein thiol levels decreased at both concentrations in liver of silver catfish and muscle of carp. In silver catfish, acetylcholinesterase (AChE) decreased in brain at 6 mg/L. Nevertheless, AChE in muscle of both species increased at 3 and 6 mg/L. IBR was standardized scores of biomarker responses and was visualized using star plots. The IBR values shown that in carp there was predominantly an induction of parameters, whereas in silver catfish there was inhibition of these responses. In this way, IBR may be a practical tool for the identification of biological alterations in fish exposed to pesticides. In the present study, IBR was efficient for comparisons of fish species using clomazone. This study may serve as a base for evaluation of other pesticides in the rice field, environment, or laboratory experiment.

  1. Effects of the herbicides clomazone, quinclorac, and metsulfuron methyl on acetylcholinesterase activity in the silver catfish (Rhamdia quelen) (Heptapteridae).

    Science.gov (United States)

    dos Santos Miron, Denise; Crestani, Márcia; Rosa Shettinger, Maria; Maria Morsch, Vera; Baldisserotto, Bernardo; Angel Tierno, Miguel; Moraes, Gilberto; Vieira, Vania Lucia Pimentel

    2005-07-01

    Fingerlings of the silver catfish (Rhamdia quelen) were exposed to three herbicides widely used in rice culture in south Brazil: clomazone, quinclorac, and metsulfuron methyl. LC50 was determined and acetylcholinesterase (AChE) activity was evaluated in brain and muscle tissue of fish exposed to different herbicide concentrations after 96h (short term). The LC50 value (nominal concentration) was 7.32 mg/L for clomazone and 395 mg/L for quinclorac, but was not obtained for metsulfuron-methyl since all fingerlings survived the highest concentration of 1200 mg/L. Brain and muscle AChE activity in unexposed fish were 17.9 and 9.08 micromol/min/g protein, respectively. Clomazone significantly inhibited AChE activity in both tissues, achieving maximal inhibition of about 83% in brain and 89% in muscle tissue. In contrast, quinclorac and metsulfuron methyl caused increases in enzyme activity in the brain (98 and 179%, respectively) and inhibitions in muscle tissue (88 and 56%, respectively). This study demonstrated short-term effects of exposure to environmentally relevant concentrations of rice field herbicides on AChE activity in brain and muscle tissue of silver catfish.

  2. The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen after transportation

    Directory of Open Access Journals (Sweden)

    Joseânia Salbego

    2014-12-01

    Full Text Available This study investigated the effects of the essential oil (EO from Lippia alba on biochemical parameters related to oxidative stress in the brain and liver of silver catfish (Rhamdia quelen after six hours of transport. Fish were transported in plastic bags and divided into three treatments groups: control, 30 µL L- 1 EO from L.alba and 40 µL L-1 EO from L.alba. Prior to transport, the fish were treated with the EO from L. alba (200 µL L -1 for three minutes, except for the control group. Fish transported in bags containing the EO did not have any alterations in acetylcholinesterase, ecto -nucleoside triphosphate diphosphohydrolase and 5'nucleotidase activity in the brain or superoxide dismutase activity in the liver. The hepatic catalase (CAT, glutathione-S-transferase (GST, glutathione peroxidase (GPx, nonprotein thiol and ascorbic acid levels were significantly lower compared to the control group. However, the hepatic thiobarbituric acid- reactive substances, protein oxidation levels and the lipid peroxidation/catalase+glutathione peroxidase (LPO/CAT+GPx ratio were significantly higher in fish transported with both concentrations of the EO, indicating oxidative stress in the liver. In conclusion, considering the hepatic oxidative stress parameters analyzed in the present experiment, the transport of previously sedated silver catfish in water containing 30 or 40 µL L-1 of EO from L. alba is less effective than the use of lower concentrations.

  3. Preferred pH of silver catfish Rhamdia quelen acclimated to different pH levels pH preferencial em jundiás Rhamdia quelen aclimatados em diferentes níveis de pH

    OpenAIRE

    Ana Paula Konzen Riffel; Sinara Jardim; Marcela Ciprandi Pires; Bruna Bertagnolli; Bruna Renata Silva Corrêa; Francine Cenzi de Ré; Francis Maria Bao Zambra; Greice Lubini; Luciano de Oliveira Garcia; Bernardo Baldisserotto

    2012-01-01

    The aim of this study was to investigate the preferred pH in silver catfish Rhamdia quelen acclimated to different pH. Fish were acclimated for one week at pH 4.2±0.1, 5.2±0.1, 6.3±0.1, 7.2±0.1, 8.0±0.1, and 9.0±0.1 and after this period, transferred to a polyethylene tube with a pH gradient from 3.5 to 10.0. The position of the fish in the pH gradient was observed 1, 6 and 12 hours after transference. Results indicated that acclimation to different pH did not change pH preference of silver c...

  4. Imazapyr+imazapic herbicide determines acute toxicity in silver catfish Rhamdia quelen.

    Science.gov (United States)

    Golombieski, Jaqueline Ineu; Sutili, Fernando Jonas; Salbego, Joseânia; Seben, Débora; Gressler, Luciane Tourem; da Cunha, Jéssyka Arruda; Gressler, Leticia Trevisan; Zanella, Renato; de Almeida Vaucher, Rodrigo; Marchesan, Enio; Baldisserotto, Bernardo

    2016-06-01

    Imazapyr (IMY) and imazapic (IMI) are imidazolinone herbicides which have been associated in a commercial formulation (Kifix(®)). To date, there are no studies on the toxicity of an IMY+IMI herbicide in fish. This work aimed to assess the acute toxicity (24 and 96h) of IMY+IMI (0, 0.488 and 4.88µg/L) towards Rhamdia quelen through hematological, biochemical, immunological, ionoregulatory and enzymatic indexes. Red blood cell count was lower at 4.88 than at 0.488µg/L (24 and 96h); mean corpuscular volume was lower than control at both concentrations (24h) and at 0.488µg/L (96h); lymphocytes declined at 4.88µg/L comparing to control (96h); and monocytes increased at 4.88µg/L (96h) in comparison with the respective control and with 4.88µg/L at 24h. Aspartate aminotransferase was higher at 0.488µg/L (96h) than the respective control and the respective concentration at 24h; uric acid reduced at 4.88µg/L comparing with 0.488µg/L (96h); and cortisol was lower at 4.88µg/L compared to 0.488µg/L and control (96h). Herbicide exposure lowered plasma bactericidal activity at both concentrations (24h) and at 0.488µg/L (96h); and plasma complement activity declined at 4.88µg/L comparing with 0.488µg/L and control (96h), and was lower at all concentrations at 96h than at 24h. Plasma K(+) levels were higher at 4.88µg/L than in the remaining groups (24 and 96h); and Na(+) levels decreased at 4.88µg/L compared to control (96h). Na(+)/K(+)-ATPase and H(+)-ATPase activities in gills were lower at 4.88µg/L comparing with control (24h) and with the respective concentration at 96h; and AChE activity in brain was higher at 0.488 and 4.88µg/L than control (24h) and the respective concentrations at 96h, while in muscle it was higher at 0.488 and 4.88µg/L than control (96h) and the respective concentrations at 24h. The present findings demonstrate that, despite IMY+IMI targets the animal-absent AHAS enzyme, such formulation displayed an acute toxic effect upon R. quelen

  5. Modulation of ERK1/2 and p38MAPK by lead in the cerebellum of Brazilian catfish Rhamdia quelen

    International Nuclear Information System (INIS)

    Lead (Pb2+) is a neurotoxic trace metal, widespread in aquatic environment that can change physiologic, biochemical and behavioral parameters in diverse fish species. Chemical exposure may drive modulation of mitogen-activated protein kinases (MAPKs) that are a family of highly conserved enzymes which comprise ubiquitous groups of signaling proteins playing critical regulatory roles in cell physiology. Extracellular signal-regulated kinases (ERK1/2) and p38MAPK control complex programs such as gene expression, embryogenesis, cell differentiation, cell proliferation, cell death and synaptic plasticity. Little information is available about MAPKs in aquatic organisms and their modulation by trace metals. The aim of this work was to determine the modulation of ERK1/2 and p38MAPK phosphorylation by Pb2+ in vivo and in vitro, in cerebellar slices of the catfish, Rhamdia quelen. In the in vitro model, slices were incubated for 3 h with lead acetate (1-10 μM). In the in vivo studies, the animals were exposed for 2 days to lead acetate (1 mg L-1). ERK1/2 and p38MAPK (total and phosphorylated forms) were immunodetected in cerebellar slices by Western blotting. Pb2+ added in vitro at 5 and 10 μM increased significantly the phosphorylation of both MAPKs. The in vivo exposed animals also showed a significant increase of ERK1/2 and p38MAPK phosphorylation without changes in the total content of the enzymes. In conclusion, the present work indicates that it is possible to evaluate the ERK1/2 and p38MAPK activation in the central nervous system (CNS) of a freshwater fish largely distributed in South America. Moreover, Pb2+, an important environmental pollutant may activate in vitro and in vivo ERK1/2 and p38MAPK enzymes. These findings are important considering the functional and ecologic implications associated to Pb2+ exposure of a freshwater fish species, such as R. quelen, and the roles of ERK1/2 and p38MAPK in the control of brain development, neuroplasticity and cell

  6. Probiotic actions of Bacillus cereus var. toyoi and Saccharomyces boulardii in silver catfish (Rhamdia quelen larvae culture

    Directory of Open Access Journals (Sweden)

    Diego Moreira de Souza

    2012-03-01

    Full Text Available The objective of this study was to evaluate the use of Bacillus cereus var. toyoi and Saccharomyces boulardii as probiotics to improve Rhamdia quelen culture. Six hundred larvaes (0.16±0.07 g were divided in three replicate tanks (25-L recirculation, 20 ºC, photoperiod of 12 h light/12 h darkness per treatment and were randomly assigned to the following treatments: Bacillus cereus var. toyoi; Saccharomyces boulardii; B. toyoi and S. boulardii; and control (without probiotic addition for a period of 30 days. The fish were fed five times daily (56% crude protein - Supra alevino inicial® and the probiotics were applied in water once a day. The doses of probiotics were 5 × 10(8 and 2 × 10(9 CFU (colony forming unit/mL for B. cereus var. toyoi and S. boulardii, respectively. Both probiotics have an inhibitory effect in vitro against Vibrio carchariae and are able to grow in media prepared with fishery water; however, no effect was observed on growth parameters when they were administered to Rhamdia quelen larvae.

  7. Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants.

    Science.gov (United States)

    Pavan, T R; Di Domenico, J; Kirsten, K S; Nied, C O; Frandoloso, R; Kreutz, L C

    2016-07-25

    Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60-80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund's complete adjuvant (FCA), Freund's incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species. PMID:27464022

  8. Early biochemical biomarkers for zinc in silver catfish (Rhamdia quelen) after acute exposure.

    Science.gov (United States)

    Leitemperger, Jossiele; Menezes, Charlene; Santi, Adriana; Murussi, Camila; Lópes, Thais; Costa, Maiara; Nogueira, Lygia Sega; Loro, Vania Lucia

    2016-06-01

    Contamination of aquatic ecosystems by metals causes various biochemical changes in aquatic organisms, and fish are recognized as indicators of environmental quality. Silver catfish were exposed to six concentrations of zinc (Zn): 1.0, 2.5, 5.0, 7.5, 10.0 and 12.5 mg/L for 96 h to determine the mean lethal concentration (LC50). The value obtained was 8.07 mg/L. In a second experiment, fish were exposed to concentrations of 1.0 or 5.0 mg/L Zn and a control for 96 h. Afterward, the tissues were collected for biochemical analysis. Lipid peroxidation, as indicated by thiobarbituric acid-reactive substance (TBARS), decreased in the liver and brain for all Zn concentrations tested, while in the gills TBARS levels increased at 1.0 mg/L and declined at 5.0 mg/L. Zn increased protein carbonyls in the muscle of silver catfish and decreased it in the other tissues. The enzyme superoxide dismutase increased in both exposed groups. However, catalase did not change. Glutathione S-transferase decreased in the liver and increased in the gills (1.0 mg/L), muscle (5.0 mg/L) and brain (1.0 and 5.0 mg/L). Nonprotein thiols changed only in brain and muscle tissue. Zn exposure inhibited acetylcholinesterase (AChE) activity in the brain at both concentrations tested, but did not change it in muscle. Exposure to Zn inhibited the activity of Na(+)/K(+)-ATPase in the gills and intestine at both concentrations tested. Our results demonstrate that Zn alters biochemical parameters in silver catfish and that some parameters such as AChE and Na(+)/K(+)-ATPase could be considered as early biomarkers of waterborne Zn toxicity. PMID:26744269

  9. Oxidative stability during frozen storage of fillets from silver catfish (Rhamdia quelen sedated with the essential oil of Aloysia triphylla during transport

    Directory of Open Access Journals (Sweden)

    Ana Paula Daniel

    2016-03-01

    Full Text Available ABSTRACT: This research aimed to evaluate whether the essential oil of Aloysia triphylla (EOAT used in vivo as a sedative in the water for transporting fish could increase the oxidative stability of silver catfish (Rhamdia quelen fillets during frozen storage. The chemical composition of EOAT and of fillets from fish exposed to EOAT (0, 30 or 40µL L-1 were assessed. The pH and lipid oxidation parameters (conjugated dienes, CD; thiobarbituric acid-reactive-substances, TBARS were evaluated in the fillets throughout the storage period (-18±2oC/17 months. The main compounds found in EOAT were α- and β-citral. Treatment with EOAT did not modify the proximate composition of the fillets, but 40µL L-1 EOAT reduced pH levels when compared to the control fillets (P<0.05. Compared to the control fillets, the fillets from fish treated with 30 and 40µL L-1 EOAT had higher initial CD values (P<0.05, whereas fillets from fish treated with 40µL L-1 EOAT had lower TBARS levels after 6, 9 and 17 months of storage (P<0.05. Results indicated that use of EOAT as a sedative in silver catfish transport water delays the degradation of primary oxidation products (CD into secondary products (TBARS in the frozen fillets. This delay in the lipid oxidation rate may increase the shelf life of frozen fillets.

  10. Preference behavior of silver catfish, Rhamdia quelen, juveniles in waters with pH gradients: laboratory experiments

    Directory of Open Access Journals (Sweden)

    Jaqueline Ineu Golombieski

    2013-09-01

    Full Text Available The aim of this study was to determine the preferred pH in silver catfish Rhamdia quelenjuveniles acclimated to different water hardness and the effect of shelters and infection by Ichthyophthirius multifiliis. Fish were acclimated for two weeks at different water hardness levels (4, 24, 50, or 100 mg CaCO3 L-1 and then transferred to a polyethylene tube with a pH gradient ranging from 3.5 to 11.7 and maintaining the same hardness. The position of the fish in the pH gradient was observed at 1, 2, 4, 6, 8, 10, and 12 h after transfer. Acclimation to different water hardness did not change pH preference of uninfected silver catfish (pH 7.30-7.83, and the presence of a shelter at the preferred pH or outside this preferred pH did not change the chosen pH range, either. Consequently silver catfish favored the acid-base regulation over shelter seeking tendency. Juveniles infected with I. multifiliis acclimated to water hardness of 24 mg CaCO3 L-1 preferred alkaline pH (9.08-9.79. This choice is not explained by the higher Na+ levels at alkaline pH compared to neutral pH because infected and uninfected fish choose the same waterborne Na+ levels in a Na+ gradient with the same pH.

  11. Effect of clomazone herbicide on biochemical and histological aspects of silver catfish (Rhamdia quelen) and recovery pattern.

    Science.gov (United States)

    Crestani, Márcia; Menezes, Charlene; Glusczak, Lissandra; dos Santos Miron, Denise; Spanevello, Roselia; Silveira, Aron; Gonçalves, Fábio Ferreira; Zanella, Renato; Loro, Vânia Lúcia

    2007-05-01

    The effects of the herbicide, clomazone, on acetylcholinesterase (AChE), catalase and TBARS formation in teleost fish (Rhamdia quelen) were studied. The fish were exposed to 0.5 or 1.0 mg L(-1) of clomazone for 12, 24, 48, 96 and 192 h. After 192 h of exposure period, fish were transferred to clean water and kept in the same for 192 h to study the recovery response. Same parameters as that of exposure period were assayed after 96 and 192 h of recovery period. Specific AChE activity was reduced in the brain and muscle after treatments, reaching a maximum inhibition of 47% in the brain and 45% in the muscle after 12h of exposure. Fish exposed to clomazone increased TBARS production in the liver for all exposure periods. The brain presented elevated TBARS levels after 12, 24 and 48 h, but after 96 and 192 h, these levels decreased. The decrease of TBARS levels persisted in brain tissue after 96 h of recovery and returned to the control value after 192 h in clean water. Catalase activity was reduced for all periods of exposure. Histological analysis showed vacuolation in the liver after herbicide exposure. Some of the alterations observed were completely restored after recovery period.

  12. Hematological and histopathological changes in silver catfish Rhamdia quelen (Siluriformes) exposed to clomazone herbicide in the Madre River, Santa Catarina State, Southern Brazil.

    Science.gov (United States)

    Brum, Aline; Dotta, Geovana; Roumbedakis, Katina; Gonçalves, Eduardo L T; Garcia, Laura P; Garcia, Patrícia; Scussel, Vildes M; Martins, Maurício L

    2014-01-01

    This study evaluated the influence of the clomazone herbicide (2-[(2-chlorophenyl)methyl]-4,4-dimethyl-3-isoxazolidinone) contamination on the hematological parameters and histological changes in gills and liver of silver catfish (Rhamdia quelen) from Madre River, Santa Catarina State, Southern Brazil. Fish were collected between March 2010 and January 2012 at two different sites of the Madre River, one site receiving residual water (contaminated site) from rice culture (n=49) and another that do not receive residual water (reference site) (n=48). The herbicide clomazone analysis detected 3.40±1.70 μg/L in the contaminated site and 1.1±0.33 μg/L in the reference site. Fish from contaminated site showed increased (Pclomazone. On the other hand, no difference was found in the hematocrit percentage, red blood cell count, total thrombocyte number, white blood cell count, lymphocytes, and neutrophils number. Fish from both sites showed histopathological changes in gills and liver, possibly caused by chronic exposure to contamination. The influence of herbicide sub doses on fish health is also discussed.

  13. Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish, Rhamdia quelen.

    Science.gov (United States)

    Parodi, Thaylise V; Cunha, Mauro A; Becker, Alexssandro G; Zeppenfeld, Carla C; Martins, Dirlaine I; Koakoski, Gessi; Barcellos, Leonardo Gil; Heinzmann, Berta M; Baldisserotto, Bernardo

    2014-04-01

    This study investigated the efficacy of the essential oil (EO) of Aloysia triphylla as an anesthetic for albino and gray strains of silver catfish, Rhamdia quelen. Juveniles were exposed to concentrations between 20 and 800 μL L(-1) EO of A. triphylla to evaluate time of induction and recovery from anesthesia. In another experiment, both strains were divided into four groups such as 0 (control), 30, 40, or 50 μL L(-1) EO and transported for 5 h. The longest time for anesthetic induction and recovery was observed in the albinos. Both strains reached anesthesia in the 100-800 μL L(-1) (11.1-1.24 min) range, without mortality, being 200 μL L(-1) the best response considering time to anesthesia (5.35 min). Albinos transported with all EO concentrations showed higher values of carbon dioxide in the water of transport, but lower levels were observed in grays transported with 40 and 50 μL L(-1) EO when compared to control fish. The same concentrations did not prevent significant whole-body cortisol rise at the end of transport in the albino strain. Juveniles of both strains transported with EO presented lower ion loss to the water compared to control fish. The EO of A. triphylla is an effective anesthetic for albino and gray silver catfish. This EO increases whole-body cortisol levels in the albino strain, but as it reduces net ion loss as in the gray strain, it can be also recommended for transport. PMID:23974669

  14. Fontes não-convencionais de proteína na primeira alimentação do bagre sul americano (Rhamdia quelen Unconventional protein sources in the first feeding of South American catfish (Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    David Roque Hernández

    2009-06-01

    Full Text Available O presente estudo teve como objetivo avaliar diferentes fontes de proteína na primeira alimentação de jundiá (Rhamdia quelen sob condições de laboratório. Foram testadas três rações balanceadas secas: ovo de peixe (TA, hidrolisado de carcaça do peixe (TB e fígado de frango cru (TC, na quais foram analisadas, pelo crescimento, a biomassa final produzida e a sobrevivência das larvas. O ensaio teve uma duração de 20 dias, e as rações foram fornecidas ad libitum. As larvas alimentadas com ovo de peixe apresentaram os melhores resultados com peso médio individual de 340mg, sobrevivência de 57% e biomassa final de 1.350mg, diferindo significativamente dos demais tratamentos. Os resultados indicaram que a formulação de rações balanceadas com ovos de peixes fornecida na primeira alimentação de larvas de jundiá pode tornar-se uma alternativa promissora na larvicultura dessa espécie.This study aimed to evaluate different proteins sources in first feeding of jundiá (Rhamdia quelen under laboratory conditions. Three balanced dry rations were tested, fish roe (TA, biological fish silage (TB and crude chicken liver (TC evaluating specific growth rate, final biomass produced and survival rate. The experiment was performed in 20 days, and all the rations were provided ad libitum. Larvae fed with fish roe presented better results, with 340mg of individual mean weight, 57% of survival rate and 1.350mg of final biomass, differing significantly with the other treatments. The results show that the inclusion of fish roe in balanced rations offered at the first feeding of jundiá larvae may become a promising alternative for the hatchering of the species.

  15. Sedative and anesthetic activities of the essential oils of Hyptis mutabilis (Rich. Briq. and their isolated components in silver catfish (Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    L.L. Silva

    2013-09-01

    Full Text Available This study evaluated the sedative and anesthetic effects of the essential oils (EO of Hyptis mutabilis (Rich. Briq. and their isolated components on silver catfish (Rhamdia quelen. Quantitative chemical differences between the EOs obtained from leaves and inflorescences were verified, and a new chemotype rich in globulol was described. Although there were no significant differences in the time of induction for sedation and anesthesia between the EOs, only the leaf EO at 344 mg/L anesthetized all fish without side effects. Fractionation of the leaf EO was carried out by column chromatography. The isolated compounds [(+-1-terpinen-4-ol and (--globulol] showed different activity from that detected for the leaf EO in proportional concentrations and similar sedation to a eugenol control at 10 mg/L. However, fish exposed to 1-terpinen-4-ol (3 and 10 mg/L did not remain sedated for 30 min. Anesthesia was obtained with 83-190 mg/L globulol, but animals showed loss of mucus during induction and mortality at these concentrations. Synergism of the depressor effects was detected with the association of globulol and benzodiazepine (BDZ, compared with either drug alone. Fish exposed to BDZ or globulol+BDZ association showed faster recovery from anesthesia in water containing flumazenil, but the same did not occur with globulol. In conclusion, the use of globulol in aquaculture procedures should be considered only at sedative concentrations of 10 and 20 mg/L, and its mechanism of action seems not to involve the GABAA-BDZ system.

  16. Modulation of ERK1/2 and p38{sup MAPK} by lead in the cerebellum of Brazilian catfish Rhamdia quelen

    Energy Technology Data Exchange (ETDEWEB)

    Leal, Rodrigo B. [Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Florianopolis, SC 88040-900 (Brazil)]. E-mail: bainyle@mbox1.ufsc.br; Ribeiro, Sandro Jose [Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Florianopolis, SC 88040-900 (Brazil); Posser, Thais [Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Florianopolis, SC 88040-900 (Brazil); Cordova, Fabiano M. [Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Florianopolis, SC 88040-900 (Brazil); Escola de Medicina Veterinaria e Zootecnia, Universidade Federal do Tocantins, Araguaina - TO 77804-970 (Brazil); Rigon, Ana Paula [Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Florianopolis, SC 88040-900 (Brazil); Filho, Evoy Zaniboni [Departamento de Aquicultura, Centro de Ciencias Agrarias, Universidade Federal de Santa Catarina, Florianopolis, SC 88040-900 (Brazil); Bainy, Afonso C.D. [Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Florianopolis, SC 88040-900 (Brazil)

    2006-04-20

    Lead (Pb{sup 2+}) is a neurotoxic trace metal, widespread in aquatic environment that can change physiologic, biochemical and behavioral parameters in diverse fish species. Chemical exposure may drive modulation of mitogen-activated protein kinases (MAPKs) that are a family of highly conserved enzymes which comprise ubiquitous groups of signaling proteins playing critical regulatory roles in cell physiology. Extracellular signal-regulated kinases (ERK1/2) and p38{sup MAPK} control complex programs such as gene expression, embryogenesis, cell differentiation, cell proliferation, cell death and synaptic plasticity. Little information is available about MAPKs in aquatic organisms and their modulation by trace metals. The aim of this work was to determine the modulation of ERK1/2 and p38{sup MAPK} phosphorylation by Pb{sup 2+} in vivo and in vitro, in cerebellar slices of the catfish, Rhamdia quelen. In the in vitro model, slices were incubated for 3 h with lead acetate (1-10 {mu}M). In the in vivo studies, the animals were exposed for 2 days to lead acetate (1 mg L{sup -1}). ERK1/2 and p38{sup MAPK} (total and phosphorylated forms) were immunodetected in cerebellar slices by Western blotting. Pb{sup 2+} added in vitro at 5 and 10 {mu}M increased significantly the phosphorylation of both MAPKs. The in vivo exposed animals also showed a significant increase of ERK1/2 and p38{sup MAPK} phosphorylation without changes in the total content of the enzymes. In conclusion, the present work indicates that it is possible to evaluate the ERK1/2 and p38{sup MAPK} activation in the central nervous system (CNS) of a freshwater fish largely distributed in South America. Moreover, Pb{sup 2+}, an important environmental pollutant may activate in vitro and in vivo ERK1/2 and p38{sup MAPK} enzymes. These findings are important considering the functional and ecologic implications associated to Pb{sup 2+} exposure of a freshwater fish species, such as R. quelen, and the roles of ERK1

  17. Dietary soybean meal on growth and intestinal morphology of South American catfish, Rhamdia quelen, larvae Farelo de soja em dietas para larvas de jundiá (Rhamdia quelen sobre o crescimento e morfologia intestinal

    Directory of Open Access Journals (Sweden)

    David Roque Hernández

    2012-09-01

    Full Text Available The effects of the replacement of bread yeast by soybean meal in diets for Rhamdia quelen larvae on growth, survival, and intestinal morphology were analysed. Larvae were fed for 20 days with five diets: a control diet formulated with 57% of bread yeast, and other four diets in which soybean meal at concentrations of 14.25, 28.5, 42.75 and 57% was added to obtain 25, 50, 75 and 100% of bread yeast replacement. Growth and survival parameters were negatively affected by dietary soybean meal inclusion. Larvae fed control diet showed significantly higher mean weight, specific growth rate, final biomass, and survival rate than larvae from other treatments. Enterocyte height and fold width of the posterior intestine showed highest values in the control group, and an inverse linear relationship with the level of dietary soybean meal inclusion was observed, however, in the anterior intestine the morphology parameters were not affected by the diet. These results indicate that inclusion of soybean meal in diets for R. quelen larvae negatively affects growth and survival, as well as the capacity for digestion and absorption of nutrients, mainly in the posterior intestine.Neste estudo, foi analisado o efeito da substituição de levedura de pão por farelo de soja em dietas para larvas de Rhamdia quelen no crescimento, sobrevivência e morfologia intestinal. As larvas foram alimentadas durante 20 dias com cinco dietas experimentais: uma dieta controle formulada com 57% de levedura de pão e quatro dietas em que o farelo de soja foi adicionado em 14,25, 28,5, 42,75 e 57% para obter 25, 50, 75 e 100% de substituição de levedura de pão. As variáveis de crescimento e sobrevivência foram negativamente afetadas pela inclusão da soja na dieta. As larvas alimentadas com a dieta controle apresentaram maior peso médio, taxa de crescimento específico, biomassa final e sobrevivência, diferindo significativamente dos demais tratamentos. A altura dos enter

  18. Avaliação de diferentes fontes protéicas sobre o desempenho inicial de larvas do jundiá Rhamdia quelen Evaluation of different sources of protein on the initial performance of south american catfish larvae Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    Rosamari Piaia

    1997-06-01

    Full Text Available O presente estudo foi realizado com o objetivo de avaliar o efeito de diferentes fontes protéicas sobre a sobrevivência e crescimento de larvas de Rhamdia quelen durante as três primeiras semanas de vida. Utilizou-se 3000 larvas distribuídas em 15 grupos, estocadas em condições controladas de cultivo, em um sistema de criação com reutilização de água, termoregulada. As rações testadas eram isoprotéicas com 35% de PB (proteína bruta e níveis energéticos variando entre 3075 e 3286kcal ED/kg, formuladas com fontes de origem animal, vegetal e pó de levedura Saccharomyces cerevisae. Agranulometria dos alimentos testados foi de 100 a 200mi, 200 a 400mi e 400 a 600mi para as três semanas experimentais, respectivamente. A distribuição do alimento foi feita a vontade entre 8 e 20 horas. No final deste experimento o tratamento T1, contendo figado bovino e levedura, resultou na maior taxa de sobrevivencia (61% apresentando diferença altamente significativa em relação aos demais tratamentos. Os tratamentos 2 (soja, levedura, 3 (soja, figado bovino, 4 (soja, milho e 5 (soja, carne, milho não diferiram significativamente entre si, apresentando baixas taxas de sobrevivência (1,17 a 10,19%. OT1 apresentou o maior comprimento total aos 21 dias, com media individual de 16,46mm, diferindo significativamente dos demais tratamentos. Nestas condições conclui-se que o pó de levedura juntamente com o fígado bovino em rações, proporcionou o melhor desempenho de larvas de jundiá Rhamdia quelen.The experiment was carried out to evaluate the survival and growth of catfish larvae Rhamdia quelen with different sources of protein during the first three weeks of life. Five treatments, with three replications, were tested. A total of 3000 larvae were randomly distributed into 15 groups, maintained under controlled conditions using a termoregulatory water re-use system. The artificial diets tested contained 35% of crude protein and digestible

  19. Biologia do jundiá Rhamdia quelen (Teleostei, Pimelodidae) Biology of Rhamdia quelen (Teleostei, Pemelodidae)

    OpenAIRE

    Levy de Carvalho Gomes; Jaqueline Ineu Golombieski; Adriana Regina Chippari Gomes; Bernardo Baldisserotto

    2000-01-01

    O jundiá, Rhamdia quelen, é encontrado desde o centro da Argentina até o sul do México, e seu cultivo está aumentando no sul do Brasil. Portanto, o objetivo desta revisão é apresentar os dados existentes até o momento sobre a biologia dessa espécie. R. quelen pode atingir 50cm de comprimento e 3kg de peso, possui hábito noturno e habita locais calmos e profundos dos rios. Os alevinos suportam água do mar a 10%o, até 9,0g/l de sal comum e pH na faixa de 4,0 a 8,5, com melhor crescimento das la...

  20. Nutritional evaluation of phosphorylated pumpkin seed (Cucurbita moschata) protein concentrate in silver catfish Rhamdia quelen (Quoy and Gaimard, 1824).

    Science.gov (United States)

    Lovatto, Naglezi de Menezes; Goulart, Fernanda Rodrigues; de Freitas, Silvandro Tonetto; Mombach, Patricia Inês; Loureiro, Bruno Bianch; Bender, Ana Betine Beutinger; Boligon, Aline Augusti; Radünz Neto, João; da Silva, Leila Picolli

    2015-12-01

    An 8-week feeding trial was conducted to evaluate the effect of replacing fish meal with pumpkin seed meal (PSM) or phosphorylated protein concentrate of pumpkin seed meal (PPCPS) on growth and metabolic responses of silver catfish. Five isonitrogenous and isocaloric diets were formulated. Control diet contained fish meal as the main protein source. The treatment groups contained 25 and 50% of either PSM or PPCPS protein replaced the fishmeal protein. A total of 400 silver catfish, with initial mean weight of 24 ± 0.46 g, were distributed into 20 tanks. For data four orthogonal contrasts were applied: control diet versus PSM diets; control diets versus PPCPS diets; control versus other diets; PSM diets versus PPCPS diets. The results indicated that the fish fed PSM diets had lower weight gain when compared to either control diet or PPCPS. The PPCPS do not affect growth and protein efficiency ratio. Lower albumin contents were found for the control diet fish for the contrasts control diet versus PPCPS diet and control diet versus other diets. The hepatic ALAT enzyme activity was higher in the fish fed the control diet (P < 0.05). The hepatic ALP was most active in fish that received the PPCPS diets, when comparing control diet versus PPCPS diets and control diet versus other diets. The hepatosomatic index was higher for fish fed the PPCPS. Our results indicated that PPCPS presents relevant nutritional quality for fish and can replace the fish meal protein up to 50% without affecting growth, PER and intermediate metabolites in silver catfish.

  1. Nutritional evaluation of phosphorylated pumpkin seed (Cucurbita moschata) protein concentrate in silver catfish Rhamdia quelen (Quoy and Gaimard, 1824).

    Science.gov (United States)

    Lovatto, Naglezi de Menezes; Goulart, Fernanda Rodrigues; de Freitas, Silvandro Tonetto; Mombach, Patricia Inês; Loureiro, Bruno Bianch; Bender, Ana Betine Beutinger; Boligon, Aline Augusti; Radünz Neto, João; da Silva, Leila Picolli

    2015-12-01

    An 8-week feeding trial was conducted to evaluate the effect of replacing fish meal with pumpkin seed meal (PSM) or phosphorylated protein concentrate of pumpkin seed meal (PPCPS) on growth and metabolic responses of silver catfish. Five isonitrogenous and isocaloric diets were formulated. Control diet contained fish meal as the main protein source. The treatment groups contained 25 and 50% of either PSM or PPCPS protein replaced the fishmeal protein. A total of 400 silver catfish, with initial mean weight of 24 ± 0.46 g, were distributed into 20 tanks. For data four orthogonal contrasts were applied: control diet versus PSM diets; control diets versus PPCPS diets; control versus other diets; PSM diets versus PPCPS diets. The results indicated that the fish fed PSM diets had lower weight gain when compared to either control diet or PPCPS. The PPCPS do not affect growth and protein efficiency ratio. Lower albumin contents were found for the control diet fish for the contrasts control diet versus PPCPS diet and control diet versus other diets. The hepatic ALAT enzyme activity was higher in the fish fed the control diet (P < 0.05). The hepatic ALP was most active in fish that received the PPCPS diets, when comparing control diet versus PPCPS diets and control diet versus other diets. The hepatosomatic index was higher for fish fed the PPCPS. Our results indicated that PPCPS presents relevant nutritional quality for fish and can replace the fish meal protein up to 50% without affecting growth, PER and intermediate metabolites in silver catfish. PMID:26377938

  2. Effects of diets containing vegetable protein concentrates on performance and activity of digestive enzymes in silver catfish (Rhamdia quelen

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    Naglezi de Menezes Lovatto

    2014-02-01

    Full Text Available The purpose of study was to evaluate the effect of using protein concentrates crambe and sunflower meal in the diet of silver catfish juveniles, as substitute for animal protein source. A total of 300 silver catfish had been separate in 15 experimental units of 280 L, totaling five treatments with three replications. We evaluated two levels (25% and 50% replacement of the meat and bone meal by protein concentrates of crambe and sunflower meals. Evaluated growth parameters, biological index and digestive enzymes in fish. There was no statistical difference for mass (g and standard length (cm, but the fish diet CPFCr-25% had greater total length (cm. No difference in dry matter, crude protein and total protein deposited (calculated. However, there was a higher concentration of ash in the carcass of the animals fed the control diet and CPFCr-50% in relation to diet CPFG- 50%, in addition, higher levels of lipids in fish fed diet CPFG-50%. No significant differences for hepatosomatic index, digestive somatic index and intestinal quotient of animals subjected to different treatments. The activity of digestive enzymes trypsin and chymotrypsin did not change. There was increased activity of acid protease. The quantitative and qualitative increase in protein concentration from this fraction allows the use of bran protein concentrates crambe and sunflower as substitutes for animal protein source.

  3. Biologia do jundiá Rhamdia quelen (Teleostei, Pimelodidae Biology of Rhamdia quelen (Teleostei, Pemelodidae

    Directory of Open Access Journals (Sweden)

    Levy de Carvalho Gomes

    2000-03-01

    Full Text Available O jundiá, Rhamdia quelen, é encontrado desde o centro da Argentina até o sul do México, e seu cultivo está aumentando no sul do Brasil. Portanto, o objetivo desta revisão é apresentar os dados existentes até o momento sobre a biologia dessa espécie. R. quelen pode atingir 50cm de comprimento e 3kg de peso, possui hábito noturno e habita locais calmos e profundos dos rios. Os alevinos suportam água do mar a 10%o, até 9,0g/l de sal comum e pH na faixa de 4,0 a 8,5, com melhor crescimento das larvas na faixa de pH de 8,0 a 8,5. É uma espécie euritérmica. Esse peixe é omnívoro, com tendência piscívora. A maturidade sexual é atingida no primeiro ano de vida. É uma espécie ovulípara e, na natureza, os cardumes desovam em locais com água limpa, calma e de fundo pedregoso. Não apresenta cuidado parental. Possui dois picos reprodutivos por ano (um no verão e outro na primavera e desova múltipla. A indução da desova apresentou bons resultados com gonadotrofina coriônica humana (HCG ou extrato hipofisário. O desenvolvimento embrionário de R. quelen é rápido e se dá entre 3 a 5 dias. O melhor alimento artificial para larvas de R. quelen é baseado em lecitina de soja, fígado bovino e levedura. Várias bactérias patogênicas e trematódeos digenéticos já foram identificados em R. quelen. Para um melhor aproveitamento dessa espécie na piscicultura, são necessários mais estudos relacionados ao efeito de parâmetros físicoquímicos da água, alimentação artificial e crescimento em cativeiro.The jundiá, Rhamdia quelen, occurs from Southern Mexico to Central Argentina, and the husbandry of this species is spreading in Southern Brazil. Consequently, the aim of this review is to present the available data of the biology of this species. R. quelen can reach 50cm of length and 3kg of weight, presents a nocturnal habit and lives in placid and deep waters of the rivers. Fingerlings support 10‰ seawater, up to 9g/l table

  4. Efeito da adição de aminoácidos essenciais à dieta sobre a secreção de enzimas digestivas de jundiá Rhamdia quelen (Siluriformes, Pimelodidae = Effect of dietary essential amino acids addition on digestive enzime secretion in silver catfish Rhamdia quelen (Siluriformes, Pimelodidae

    Directory of Open Access Journals (Sweden)

    Alexandre Bernardini Ungar

    2009-01-01

    Full Text Available Em estudos realizados com variação no teor de proteína na ração para peixes, foi demonstrado o aumento das atividades de protease alcalina, protease ácida, tripsina e quimiotripsina do trato digestório de jundiá (Rhamdia quelen. Esses fatos sugerem que produtos liberados pela digestão na luz desse trato podem influenciar a síntese e a secreção de enzimas digestivas. A secreção destas enzimas pelo pâncreas, em vertebrados, responde à influência neurale humoral. No presente trabalho, foram testadas quatro concentrações (0, 3, 6 e 12% de uma mistura de aminoácidos (treonina, fenilalanina, leucina, valina, arginina e triptofano em iguaisproporções em dietas isocalóricas contendo 3.500 kcal de energia digestível kg-1 de ração, e dietas isoproteicas contendo 20% de proteína, em juvenis de jundiá. Foram utilizados dez animais portratamento, sendo os peixes estocados num sistema fechado com recirculação de água e temperatura controlada. Os peixes submetidos a estes tratamentos apresentaram atividade da protease alcalina na região anterior do intestino, responsiva aos diferentes níveis aminoácidos da alimentação. Todavia, as atividades protease alcalina do fígado e amilase do fígado e intestino anterior não foram detectadas. Estes resultados sugerem que a atividade protease alcalina do intestino anterior seja induzida por aminoácidos liberados na luz do trato digestório. Studies carried out with variation of protein concentration in the fish feed have shown an increase in the activity of alkaline proteases, acid proteases, trypsin and chimotrypsin from the digestive tract of silver catfish (Rhamdia quelen. These facts suggest that products released by digestion in the lumen of this tract can influenced the synthesis and secretion of digestive enzymes. The secretion of these enzymes by the pancreas in vertebrate responses to neural and humoral influences. In the present study, four concentration (0, 3, 6 and 12% of

  5. Efeito da adição de aminoácidos essenciais à dieta sobre a secreção de enzimas digestivas de jundiá Rhamdia quelen (Siluriformes, Pimelodidae - DOI: 10.4025/actascibiolsci.v31i1.822 Effect of dietary essential amino acids addition on digestive enzime secretion in silver catfish Rhamdia quelen (Siluriformes, Pimelodidae - DOI: 10.4025/actascibiolsci.v31i1.822

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    Alexandre Eneas Altran

    2009-04-01

    Full Text Available Em estudos realizados com variação no teor de proteína na ração para peixes, foi demonstrado o aumento das atividades de protease alcalina, protease ácida, tripsina e quimiotripsina do trato digestório de jundiá (Rhamdia quelen. Esses fatos sugerem que produtos liberados pela digestão na luz desse trato podem influenciar a síntese e a secreção de enzimas digestivas. A secreção destas enzimas pelo pâncreas, em vertebrados, responde à influência neural e humoral. No presente trabalho, foram testadas quatro concentrações (0, 3, 6 e 12% de uma mistura de aminoácidos (treonina, fenilalanina, leucina, valina, arginina e triptofano em iguais proporções em dietas isocalóricas contendo 3.500 kcal de energia digestível kg-1 de ração, e dietas isoproteicas contendo 20% de proteína, em juvenis de jundiá. Foram utilizados dez animais por tratamento, sendo os peixes estocados num sistema fechado com recirculação de água e temperatura controlada. Os peixes submetidos a estes tratamentos apresentaram atividade da protease alcalina na região anterior do intestino, responsiva aos diferentes níveis aminoácidos da alimentação. Todavia, as atividades protease alcalina do fígado e amilase do fígado e intestino anterior não foram detectadas. Estes resultados sugerem que a atividade protease alcalina do intestino anterior seja induzida por aminoácidos liberados na luz do trato digestório.Studies carried out with variation of protein concentration in the fish feed have shown an increase in the activity of alkaline proteases, acid proteases, trypsin and chimotrypsin from the digestive tract of silver catfish (Rhamdia quelen. These facts suggest that products released by digestion in the lumen of this tract can influenced the synthesis and secretion of digestive enzymes. The secretion of these enzymes by the pancreas in vertebrate responses to neural and humoral influences. In the present study, four concentration (0, 3, 6 and 12

  6. Anesthetic activity and bio-guided fractionation of the essential oil of Aloysia gratissima (Gillies & Hook. Tronc. in silver catfish Rhamdia quelen

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    SIMONE C. BENOVIT

    2015-09-01

    Full Text Available This work aimed to determine the efficacy of the essential oil of A. gratissima as anesthetic for silver catfish, and to perform the bio-guided fractionation of essential oil aiming to isolate compounds responsible for the noted effects. Fish were submitted to anesthesia bath with essential oil, its fractions and isolated compounds to determine time of anesthetic induction and recovery. Eugenol (50 mg L-1 was used as positive control. Essential oil of A. gratissima was effective as an anesthetic at concentrations of 300 to 900 mg L-1. Fish presented involuntary muscle contractions during induction and recovery. The bio-guided fractionation of essential oil furnishedE-(--pinocamphone, (--caryophyllene oxide, (--guaiol and (+-spathulenol. E-(--pinocamphone caused the same side effects observed for essential oil. (--Caryophyllene oxide, (--guaiol and (+-spathulenol showed only sedative effects at proportional concentrations to those of the constituents in essential oil. (+-Spathulenol (51.2 mg L-1 promoted deep anesthesia without side effects. A higher concentration of (+-spathulenol, and lower or absent amounts ofE-(--pinocamphone could contribute to increase the activity and safety of the essential oil of A. gratissima. (+-Spathulenol showed potent sedative and anesthetic activities in silver catfish, and could be considered as a viable compound for the development of a new anesthetic.

  7. Anesthetic activity and bio-guided fractionation of the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. in silver catfish Rhamdia quelen.

    Science.gov (United States)

    Benovit, Simone C; Silva, Lenise L; Salbego, Joseânia; Loro, Vania L; Mallmann, Carlos A; Baldisserotto, Bernardo; Flores, Erico M M; Heinzmann, Berta M

    2015-09-01

    This work aimed to determine the efficacy of the essential oil of A. gratissima as anesthetic for silver catfish, and to perform the bio-guided fractionation of essential oil aiming to isolate compounds responsible for the noted effects. Fish were submitted to anesthesia bath with essential oil, its fractions and isolated compounds to determine time of anesthetic induction and recovery. Eugenol (50 mg L(-1)) was used as positive control. Essential oil of A. gratissima was effective as an anesthetic at concentrations of 300 to 900 mg L(-1). Fish presented involuntary muscle contractions during induction and recovery. The bio-guided fractionation of essential oil furnished E-(-)-pinocamphone, (-)-caryophyllene oxide, (-)-guaiol and (+)-spathulenol. E-(-)-pinocamphone caused the same side effects observed for essential oil. (-)-Caryophyllene oxide, (-)-guaiol and (+)-spathulenol showed only sedative effects at proportional concentrations to those of the constituents in essential oil. (+)-Spathulenol (51.2 mg L(-1)) promoted deep anesthesia without side effects. A higher concentration of (+)-spathulenol, and lower or absent amounts ofE-(-)-pinocamphone could contribute to increase the activity and safety of the essential oil of A. gratissima. (+)-Spathulenol showed potent sedative and anesthetic activities in silver catfish, and could be considered as a viable compound for the development of a new anesthetic. PMID:26221984

  8. Oxidative stress parameters in silver catfish (Rhamdia quelen) juveniles infected with Ichthyophthirius multifiliis and maintained at different levels of water pH.

    Science.gov (United States)

    Garcia, L O; Becker, A G; Bertuzzi, T; Cunha, M A; Kochhann, D; Finamor, I A; Riffel, A P K; Llesuy, S; Pavanato, M A; Baldisserotto, B

    2011-05-31

    The aim of this study was to determine oxidative stress parameters in the liver, gill and muscle of silver catfish juveniles infected with Ichthyophthirius multifiliis and maintained at pH 5.0 or 7.0 for three days. Juveniles were infected by adding one I. multifiliis-infected juvenile and water containing theronts to tanks. After the appearance of white spots on the skin, infected juveniles exposed to pH 5.0 and 7.0 showed significantly higher thiobarbituric acid reactive substances (TBARS) levels in the liver and gills compared to uninfected juveniles. Liver of infected juveniles exposed to pH 7.0 showed higher catalase (CAT) and lower glutathione-S-transferase (GST) activities, but those maintained at pH 5.0 showed significantly higher GST activity than uninfected juveniles. The gills of infected juveniles showed significantly higher CAT (day two) and GST activity at both pH 5.0 and 7.0 compared to uninfected juveniles. Muscle of infected juveniles showed significantly lower CAT and GST activity and TBARS levels (at day three) when maintained at both pH 5.0 and 7.0 compared to uninfected juveniles. In conclusion, I. multifiliis infection induces liver and gill damage via lipid peroxidation products in silver catfish, but higher antioxidant enzyme activity could indicate a greater degree of protection against this parasite.

  9. Clinical, biochemical and haemathological effects in Rhamdia quelen exposed to cypermethrin

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    F.P. Montanha

    2014-06-01

    Full Text Available The acute intoxication of Cypermethrin in Silver Catfish (Rhamdia quelen was evaluated. Animals weighing 56.67±4.43g and measuring 18.92±1.16cm were exposed to sublethal concentrations of Cypermethrin for the species in hydrological conditions during 96 hours. A total of 52 fish divided into three groups were used and received the following concentrations of Cypermethrin: 0 (n=12; 1.5 (n=20 and 2.5 (n=20mg/L. The intoxicated animals suffered behavioral changes such as loss of balance, swimming alteration, dyspnea, upright swimming and sudden spiral swimming movements. As soon as the 96-hour period was over, a blood collection for hematological and biochemical analyses was performed. A complete haemogram test, plasmatic protein test, albumin, alanine transaminase (ALT, aspartate aminotransferase (AST, gamma glutamyltransferase and alkaline phosphatase (ALP were studied. The values of erythrocytes, hematocrits, haemoglobin, total number of leukocytes, thrombocyte, ALT, AST and ALP changed according to the groups. The results have shown that the environmental contamination by Cypermethrin is toxic to the species.

  10. Exposure to different glyphosate formulations on the oxidative and histological status of Rhamdia quelen.

    Science.gov (United States)

    Murussi, Camila R; Costa, Maiara D; Leitemperger, Jossiele W; Guerra, Luciana; Rodrigues, Cintia C R; Menezes, Charlene C; Severo, Eduardo S; Flores-Lopes, Fábio; Salbego, Joseânia; Loro, Vania L

    2016-04-01

    Due to the wide use of glyphosate (GLY) in soybean cultivation, their residues in the environment may affect non-target organisms such as fish, developing toxic effects. Despite GLY being widely used in Brazil, there are few studies comparing the effects of commercial formulations in native freshwater fish species. Silver catfish (Rhamdia quelen) were exposed to three different commercial formulations of GLY 48 % (Orium(®), Original(®) and Biocarb(®)) at 0.0, 2.5 and 5.0 mg/L for 96 h. The effects in thiobarbituric acid-reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST) and histological alterations were analysed in the liver, whereas alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were studied in the plasma. In the liver, TBARS levels increased and CAT decreased in all treatments and herbicides tested in comparison with the control group. The SOD increased at 2.5 mg/L of Orium(®), Original(®) and 5.0 mg/L Orium(®) and Biocarb(®), whereas GST increased at 2.5 mg/L Orium(®) and decreased at 2.5 mg/L Biocarb(®) when compared to the control group. The main histopathological alterations in hepatic tissue were vacuolisation, leucocyte infiltration, degeneration of cytoplasm and melanomacrophage in all GLY treatments. The ALT decreased after exposure to 2.5 mg/L of Biocarb(®) and AST increased at 2.5 mg/L of Orium(®), Original(®) and 5.0 mg/L of Biocarb(®) in comparison with the control group. In summary, the oxidative damage generated by GLY may have caused the increased formation of free radicals that led to the histological alterations observed in hepatocytes. PMID:26508170

  11. Inoculação de suspensão bacteriana de Plesiomonas shigelloides em Jundiá, Rhamdia quelen (Teleostei: Pimelodidae Inoculation of bacterial suspension of Plesiomonas shigelloides in jundiá, Rhamdia quelen (Teleostei: Pimelodidae

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    Cheila de Lima Boijink

    2001-06-01

    Full Text Available Com o crescimento da aqüicultura mundial e intensificação da criação de peixes, os animais ficam sujeitos às enfermidades bacterianas e outras. Com o objetivo de avaliar a patogenicidade da Plesiomonas shigelloides para jundiás (Rhamdia quelen, diferentes concentrações bacterianas (3 x 10(8 e 9 x 10(8 UFC - Unidade Formadora de Colônia/ml foram inoculadas por via intraperitoneal. Foram utilizados 84 jundiás juvenis com peso e comprimento médios de 24,37 ± 4,28g e 14,42 ± 1,62cm, respectivamente. Os animais inoculados foram mantidos durante 21 dias, em caixas d'agua de amianto, em condições semelhantes de temperatura, pH, alcalinidade e dureza. Os jundiás foram sacrificados a cada dois dias para contagem de UFC/ml de tecido renal. Por observações diárias, constatou-se que a inoculação intraperitoneal de Plesiomonas shigelloides não ocasionou nenhuma alteração nos jundiás, independente da concentração inoculada. As contagens das bactérias nos rins dos jundiás mantiveram-se entre 10(5 e 10(6UFC/ml até o 21º dia, quando o experimento foi finalizado.As worldwide aquaculture has grown, and intensification in fish raising, the animals are subject to bacterial diseases and others. With the aim of evaluating pathogenicity of Plesiomonas shigelloides for "jundiá" (Rhamdia quelen, different bacterial concentrations (3 x 10(8 e 9 x 10(8 CFU - Colony Former Unit/ml were inoculated via peritoneum. Eigthy four juvenile "jundiá" averaging 24.37 ± 4,28g of weight and 14.42 ± 1,62cm of length were utilized. The inoculated animals were maintained for 21 days, in asbestos water tanks, at similar temperature, pH, alkalinity and hardness conditions. The "jundiás" were slaughtered every other day for counting UFC/ml renal tissue. For daily inspections, it was observed that intraperitoneal inoculation of Plesiomonas shigelloides did not cause any change in the catfishes, regardless inoculated concentration. Bacteria counting in

  12. Ultrastructural studies of Henneguya rhamdia n. sp. (Myxozoa) a parasite from the Amazon teleost fish, Rhamdia quelen (Pimelodidae).

    Science.gov (United States)

    Matos, Edilson; Tajdari, Jessica; Azevedo, Carlos

    2005-01-01

    Henneguya rhamdia n. sp. is described in the gill filaments of the teleost fish Rhamdia quelen, collected from the Peixe Boi River, State of Pará, Brazil. This myxosporean produced spherical to ellipsoidal plasmodia, up to 300 microm in diameter, which contained developmental stages, including spores. Several dense bodies up to 2 microm in diameter were observed among the spores. The spore body was ellipsoidal (13.1 microm in length, 5.2 microm in width, and 2.5 microm in thickness) and each of the two valves presented a tapering tail (36.9 microm in length). These valves surrounded the binucleated sporoplasm cell and two equal ellipsoidal polar capsules (4.7 x 1.1 microm), which contained 10-11 (rarely 12) polar filament coils. The sporoplasm contained sporoplasmosomes with a laterally eccentric dense structure with a half-crescent section. Based on the data obtained by electron microscopy and on the host specificity, the spores differed from previously described Henneguya species, mainly in their shape and size, number and arrangement of the polar filament coils, and sporoplasmosome morphology.

  13. Characterization of the ovary fatty acids composition of Rhamdia quelen (Quoy & Gaimard (Teleostei: Siluriformes, throughout their reproductive cycle

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    Rodrigo Vargas Anido

    2015-06-01

    Full Text Available Knowledge about gonad fatty acid composition is important for broodstock diet formulation. This study characterized ovary fatty acid composition of wild female jundiá catfish (Rhamdia quelen in their different gonadal maturation stages. Female jundiá (n = 36, average weight= 383.8 + 208.8 g were captured in the rio Uruguay, comprising all seasons. Ovaries were extracted and classified according to their gonadal maturation stage. Gonad-somatic ratio varied significantly among seasons, being higher in spring (3.7, followed by summer (2.2, winter (0.9 and autumn (0.6. Main fatty acids groups detected were: saturated (SFA= 35.5%, monounsaturated (MUFA= 28.1% and polyunsaturated fatty acids (PUFA= 33.5%. Over the four seasons, palmitic acid was recorded in large quantities, followed by docosahexaenoic acid (DHA and arachidonic acid (ARA. ARA was present in higher concentrations in immature or maturing ovaries, and its content decreased along the maturation process. Conversely, DHA and eicosapentaenoic acid (EPA contents increased during maturation. Such variation resulted in an increase in EPA/ARA and DHA/ARA ratios in mature gonads, which can be important for successful breeding. Such findings suggest that jundiá broodstock diets should contain lipids that provide long chain polyunsaturated fatty acids from both the n-3 and n-6 series to ensure gonadal maturation completion.

  14. Cross-amplification of heterologous microsatellite markers in Rhamdia quelen and Leporinus elongatus

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    Nelson Mauricio Lopera-Barrero

    2016-02-01

    Full Text Available Native fish species in Brazil are an asset in fish farming, but their natural stocks have been significantly reduced in recent years. To mitigate this negative impact, studies on fish conservation are being conducted and genetic tools for the discrimination of population parameters are increasingly achieving great importance. Current analysis evaluates a set of microsatellite heterologous primers in the jundiá (Rhamdia quelen and in the piapara (Leporinus elongatus. Samples from the caudal fin of 15 broodstock from each species were analyzed. DNA extraction was performed with NaCl protocol and the integrity of the extracted DNA was checked with agarose gel 1%. Twenty primers developed for Piaractus mesopotamicus, Colossoma macropomum, Prochilodus lineatus, Brycon opalinus and Oreochromis niloticus were evaluated. Cross amplification of four primers of the B. opalinus and P. lineatus species (BoM12, Pli43 and Pli60 in R. quelen and BoM2, Pli43 and Pli60 in L. elongatus was assessed. Primers of P. mesopotamicus, C. macropomum and O. niloticus showed no cross amplification in the two species analyzed. Results revealed the possibility of using the four amplified heterologous primers in genetic studies for R. quelen and L. elongatus.

  15. Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy & Gaimard, 1824 and Leporinus obtusidens (Valenciennes, 1837

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    Vania Lucia Loro

    2015-03-01

    Full Text Available Rhamdia quelen (silver catfish and Leporinus obtusidens (piava were exposed to a commercial formulation Roundup(r, a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT and aspartate aminotransferase (AST activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase activities. Plasmatic glucose levels were reduced in both species, whereas the transaminase activities (ALT and AST increased after exposure to the herbicide. Herbicide exposure increased protein and glucose levels in the mucus layer in both species. There was a reduction in both NTPDase and ecto-5'-nucleotidase activity in the brain of piava, and increased enzyme activity in silver catfish at both concentrations tested. The species showed an increase in protein carbonyl in the liver after exposure to both concentrations of the glyphosate. Our results demonstrated that exposure to Roundup(r caused liver damage, as evidenced by increased plasma transaminases and liver protein carbonyl in both of the fish species studied. The mucus composition changed and hypoglycemia was detected after Roundup(r exposure in both species. Brain nucleotide hydrolysis showed a different response for each fish species studied. These parameters indicated some important and potential indicators of glyphosate contamination in aquatic ecosystems.

  16. A new species of Aphanoblastella (Monogenea: Dactylogyridae parasitic on Rhamdia quelen (Siluriformes: Heptapteridae from Southeastern Brazil = Nova espécie de Aphanoblastella (Monogenea: Dactylogyridae parasito de Rhamdia quelen (Siluriformes: Heptapteridae do sudeste do Brasil

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    Adriano Reder de Carvalho

    2009-07-01

    Full Text Available Aphanoblastella juizforense sp. nov. is described in the gills of the heptapterid fish Rhamdia quelen (Quoy et Gaimard, 1824 from the Paraibuna River, Juiz de Fora, Minasn Gerais State, Brazil. The new species can be separated from all congeners by the long coiledcopulatory organ with one counterclockwise ring; sinuous accessory piece and for the robust shape of the ventral bar.Aphanoblastella juizforense sp. nov. é descrita parasitando as brânquias de Rhamdia quelen (Quoy et Gaimard, 1824 do rio Paraibuna, Juiz de Fora, Estado de Minas Gerais, Brasil. A nova espécie podeser diferenciada de todas as outras espécies do gênero por apresentar o órgão copulatório alongado, em forma de espiral, em sentido anti-horário, com peça acessória sinuosa e pela constituição mais robusta da barra ventral.

  17. Reproductive biology of Liso Rhamdia quelen (pisces: heptapteridae) in the Sinu River, Colombia

    International Nuclear Information System (INIS)

    The reproductive biology of Liso (Rhamdia quelen) in the Sinu River was studied. The fishes were collected between January and December 2005, with lengths ranging 15,5 - 37 cm of total length (TL) and 28 - 486 g of total weight. the gonads were placed in Gilson solution, the Vazzoler scale was applicated and sexual proportion, sexual maturity index, spawning season, the length at first maturity, ovocites diameter and fecundity were estimated; 207 females, 130 males and 1 undifferentiated were evaluated, with sexual proportion female: male of 1,6:1, different from expected. The length at first maturity was estimated in 24 cm TL for combined sexes; average ovocites diameter was 963 mm and average fecundity was estimated in 26.305 ovocites, with better equation f=2960,61 0,88, r=0,94, n=125. The Liso is a fish with asynchronous oocyte development in more than two groups, with partial spawning throughout the year.

  18. Pre-sedation and transport of Rhamdia quelen in water containing essential oil of Lippia alba: metabolic and physiological responses.

    Science.gov (United States)

    Becker, Alexssandro G; Parodi, Thaylise V; Zeppenfeld, Carla C; Salbego, Joseânia; Cunha, Mauro A; Heldwein, Clarissa G; Loro, Vania L; Heinzmann, Berta M; Baldisserotto, Bernardo

    2016-02-01

    The effects of transporting silver catfish (Rhamdia quelen) for 6 h in plastic bags containing 0 (control), 30 or 40 µL/L of essential oil (EO) from Lippia alba leaves were investigated. Prior to transport, the fish in the two experimental groups were sedated with 200 µL/L of EO for 3 min. After transport, dissolved oxygen, carbon dioxide, alkalinity, water hardness, pH, temperature and un-ionized ammonia levels in the transport water did not differ significantly among the groups. However, total ammonia nitrogen levels and net Na(+), Cl(-) and K(+) effluxes were significantly lower in the groups transported with EO of L. alba than those in the control group. PvO2, PvCO2 and HCO3(-) were higher after transporting fish in 40 µL/L of EO of L. alba, but there were no significant differences between groups regarding blood pH or hematocrit. Cortisol levels were significantly higher in fish transported in 30 µL/L of EO of L. alba compared to those of the control group. The metabolic parameters (glycogen, lactate, total amino acid, total ammonia and total protein) showed different responses after adding EO to the transport water. In conclusion, while the EO of L. alba is recommended for fish transport in the conditions tested in the present study because it was effective in reducing waterborne total ammonia levels and net ion loss, the higher hepatic oxidative stress in this species with the same EO concentrations reported by a previous study led us to conclude that the 10-20 µL/L concentration range of EO and lack of pre-sedation before transport are more effective.

  19. Pre-sedation and transport of Rhamdia quelen in water containing essential oil of Lippia alba: metabolic and physiological responses.

    Science.gov (United States)

    Becker, Alexssandro G; Parodi, Thaylise V; Zeppenfeld, Carla C; Salbego, Joseânia; Cunha, Mauro A; Heldwein, Clarissa G; Loro, Vania L; Heinzmann, Berta M; Baldisserotto, Bernardo

    2016-02-01

    The effects of transporting silver catfish (Rhamdia quelen) for 6 h in plastic bags containing 0 (control), 30 or 40 µL/L of essential oil (EO) from Lippia alba leaves were investigated. Prior to transport, the fish in the two experimental groups were sedated with 200 µL/L of EO for 3 min. After transport, dissolved oxygen, carbon dioxide, alkalinity, water hardness, pH, temperature and un-ionized ammonia levels in the transport water did not differ significantly among the groups. However, total ammonia nitrogen levels and net Na(+), Cl(-) and K(+) effluxes were significantly lower in the groups transported with EO of L. alba than those in the control group. PvO2, PvCO2 and HCO3(-) were higher after transporting fish in 40 µL/L of EO of L. alba, but there were no significant differences between groups regarding blood pH or hematocrit. Cortisol levels were significantly higher in fish transported in 30 µL/L of EO of L. alba compared to those of the control group. The metabolic parameters (glycogen, lactate, total amino acid, total ammonia and total protein) showed different responses after adding EO to the transport water. In conclusion, while the EO of L. alba is recommended for fish transport in the conditions tested in the present study because it was effective in reducing waterborne total ammonia levels and net ion loss, the higher hepatic oxidative stress in this species with the same EO concentrations reported by a previous study led us to conclude that the 10-20 µL/L concentration range of EO and lack of pre-sedation before transport are more effective. PMID:26297516

  20. Performance of jundiá larvae, Rhamdia quelen, fed on probiotic supplemented diets

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    Vilson Borba Pinto

    2015-08-01

    Full Text Available Since probiotics have proved to be a viable alternative to antibiotics as enhancers of animal growth, the performance, uniformity and mortality rates of the jundiá (Rhamdia quelen larvae fed on diets with different probiotics were evaluated. Jundiá larvae, aged four days post hatching, were fed during 21 days with the following diets, in four replicates, namely, CO: control feed, without probiotics; PP: feed with Pichia pastoris; SB: feed with Saccharomyces boulardii; BT: feed with Bacillus cereus var. toyoi. Among the tested probiotic, Bacillus cereus var. toyoi had the best results due to the fact that the larvae were 25% heavier than CO at the end of the first week; the difference increased to 28% by the end of the trial. Further, BT also improved uniformity and Fulton’s condition factor. Larvae fed on Saccharomyces boulardii had the lowest body weight, whereas those fed on Pichia pastoris grew similarly to the control diet. Mortality rate was not affected by treatments. Bacillus cereus var. toyoi improves the performance and uniformity of the larvae, but does not affect mortality rate.

  1. Características hematológicas de teleósteos brasileiros: IV. Variáveis do jundiá Rhamdia quelen ( Pimelodidae Haematological characteristics of brazilian teleosts: IV. Parameters of jundiá Rhamdia quelen (Pimelodidae

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    Marcos Tavares-Dias

    2002-08-01

    Full Text Available As características hematológicas do jundiá Rhamdia quelen Quoy & Gaimard, 1824 (Osteichthyes: Pimelodidae, oriundo de cativeiro foram estudadas. Determinou-se o valor médio da contagem de eritrócitos, taxa de hemoglobina, hematócrito, volume corpuscular médio (VCM, concentração de hemoglobina corpuscular média (CHCM e distribuição percentual de células sangüíneas de defesa orgânica. As células sangüíneas de defesa orgânica (leucócitos e trombócitos foram identificadas e caracterizadas morfologicamente. Trombócitos e linfócitos foram as células sangüíneas de defesa orgânica mais freqüente nas extensões sangüíneas do R. quelen. A análise de regressão mostrou correlação linear negativa entre o percentual de trombócitos e linfócitos. Os resultados deste estudo fornecem valores sangüíneos normais em R. quelen, em cultivo intensivo, que poderão servir de comparação com dados dessa espécie em outras situações de cultivo.The present paper describes haematological characteristics of jundiá Rhamdia quelen Quoy & Gaimard, 1824 (Osteichthyes: Pimelodidae, reared in captivity. The average values of red blood cells count, hematocrit, hemoglobin rate, mean corpuscular volume (MCV, mean corpuscular hemoglobin concentration (MCHC and defense blood cells (leukocytes and thrombocytes, were determined. Identification and morphology of organic defense blood cells were reported. Thrombocytes and lymphocytes were the most frequent cells in slides blood of R. quelen. Linear regression analysis showed negative correlation between thrombocytes and lymphocytes percentual. The present results furnish normal blood values of R. quelen reared intensively, that assist in comparison to the other culture conditions.

  2. Anesthetic activity of the essential oil of Ocimum americanum in Rhamdia quelen (Quoy & Gaimard, 1824 and its effects on stress parameters

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    Lenise de Lima Silva

    2015-12-01

    Full Text Available ABSTRACT The aim of this study was to evaluate the anesthetic activity of the essential oil (EO of Ocimum americanum L. in silver catfish (Rhamdia quelen . In the first experiment, the depressor effects and chemical composition of the leaf EO (LEO and inflorescence EO (IEO were compared. Juveniles (n = 10 were placed in aquaria containing different concentrations of EO (25 - 500 mg L-1 to determine the point at which anesthesia was induced and the length of the recovery period. In the following experiment, the effects of 300 and 500 mg L-1 LEO exposure on stress parameters (plasma cortisol, glucose and sodium levels after air exposure for 1 min were assayed. Fish (n = 10 per sampling time were sampled immediately or transferred to anesthetic-free aquaria until sampling (15, 30, 60 or 240 min. LEO was composed mainly of β-linalool and 1,8-cineole in similar proportions, whereas IEO showed β-linalool as major compound. Anesthesia was obtained in silver catfish with 200-500 mg L-1 between 4-8 min for LEO and 6-16 min for IEO. Lower EO concentrations did not reach anesthetic stage up to 30 min. LEO used as anesthetic prevented the cortisol increase and sodium loss induced by aerial exposure. Glucose levels were raised in catfish exposed to LEO compared to basal group (not air exposed in almost all observation times. EO of O. americanum obtained from leaves was considered suitable to anesthetic procedures due to its fast induction and handling-induced stress prevention.

  3. Tratamentos terapêuticos convencionais no controle do ectoparasita Ichthyophtirius multifiliis em jundiá (Rhamdia quelen Conventional therapeutic treatments on the control of the parasite Ichthyophtirius multifiliis in Rhamdia quelen

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    Paulo César Falanghe Carneiro

    2005-01-01

    Full Text Available O objetivo deste trabalho foi testar a eficiência de tratamentos convencionais no controle da infestação do ictio no jundiá (Rhamdia quelen. Vinte alevinos (3-6 cm foram estocados em 28 aquários (10 L durante 192 horas e submetidos aos seguintes tratamentos: controle, formalina comercial (0,2 mL L-1, permanganato de potássio (1,3 mg L-1, sulfato de cobre (0,63 mg L-1, sal comum (10 g L-1, verde de malaquita (0,05 mg L-1 e elevação da temperatura para 32°C. O sulfato de cobre, o sal e a elevação da temperatura reduziram o número de parasitas ao final do experimento, podendo ser utilizados como substitutos do verde de malaquita no controle do ictio no jundiá.The objective of this work was to test the efficiency of conventional treatments to control ich infection in "jundiá" (Rhamdia quelen fingerlings. Twenty fish (3-6 cm were placed in 28 aquaria (10 L for 192 hours, and submitted to the following treatments: control, formalin (0.2 mL L-1, potassium permanganate (1.3 mg L-1, copper sulfate (0.63 mg L-1, common salt (10 g L-1, malachite green (0.05 mg L-1 and temperature elevation (32°C. Copper sulfate, common salt and the elevation of temperature reduced the number of parasites in "jundiá". These treatments can substitute malachite green to control ich in this species.

  4. Influência da dureza e do ph no desenvolvimento do jundiá (Rhamdia quelen na fase de fertilização até a produção de pós-larvas

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    Graeff, Álvaro

    2007-09-01

    Full Text Available ResumoO jundiá (Rhamdia quelen, uma espécie nativa de Santa Catarina, bem como de outras regiões da América do Sul, encontra-se entre as espécies que mais se destacam para consumo , sendo hoje objeto de muitas pesquisas. O local de trabalho foi o Laboratório de Reprodução da Unidade de Pesquisa de Piscicultura da Epagri, tendo iniciado em 29 de agosto de 2005 no momento da fertilização (em torno de 2 horas em 27 incubadoras (garrafas PET com capacidade para 2,0 litros de água, abastecidos individualmente com água derivada de um poço artesiano. E encerrado em 2 de setembro de 2005 quando foram drenadas, as incubadoras e contadas as pós-larvas, perfazendo um período de 5 dias. O delineamento experimental foi inteiramente ao acaso em um esquema fatorial 3 x 3 x 3 (tabela 1 em que se testou três níveis de dureza (20, 40, 60 mg/L de CaCO3, três níveis de pH (7,0; 7,5; 8,0 com três repetições, com 1 grama de ovos fertilizados de jundiá (Rhamdia quelen em cada unidade experimental. Na analise de variância houve interação entre pH e dureza pelo teste F (P<0,05 em função disto as variáveis foram estudadas em conjunto através do teste Tukey. O efeito da dureza, tanto na fase de fertilização como desenvolvimento da larva de Jundiá (Rhamdia quelen depende do pH da água. Portanto o melhor pH na água para a indução a fertilização é 7,5 a 8,0 com dureza de 40 mg/L de CaCO3 enquanto que para produção de pós-larvas de jundiá (Rhamdia quelen o melhor pH é 8,0 com dureza de 40 mg/L de CaCO3. AbstractThe silver catfish (Rhamdia quelen, a native specie of Santa Catarina, as other South America’s regions, finds itself between the most outstanding species for consumption, being target of many researches. The workstation was the Laboratory of reproduction of the unit of research of Pisciculture of Epagri, being initiated in 29 August of 2005 in the moment of fertilization (around 2 hours in 27 incubators (PET bottles with

  5. DNA damage in the kidney tissue cells of the fish Rhamdia quelen after trophic contamination with aluminum sulfate

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    Tatiane Klingelfus

    2015-01-01

    Full Text Available Abstract Even though aluminum is the third most common element present in the earth's crust, information regarding its toxicity remains scarce. It is known that in certain cases, aluminum is neurotoxic, but its effect in other tissues is unknown. The aim of this work was to analyze the genotoxic potential of aluminum sulfate in kidney tissue of the fish Rhamdia quelen after trophic contamination for 60 days. Sixty four fish were subdivided into the following groups: negative control, 5 mg, 50 mg and 500 mg of aluminum sulfate per kg of fish. Samples of the posterior kidney were taken and prepared to obtain mitotic metaphase, as well as the comet assay. The three types of chromosomal abnormalities (CA found were categorized as chromatid breaks, decondensation of telomeric region, and early separation of sister chromatids. The tests for CA showed that the 5 mg/kg and 50 mg/kg doses of aluminum sulfate had genotoxic potential. Under these treatments, early separation of the sister chromatids was observed more frequently and decondensation of the telomeric region tended to increase in frequency. We suggest that structural changes in the proteins involved in DNA compaction may have led to the decondensation of the telomeric region, making the DNA susceptible to breaks. Moreover, early separation of the sister chromatids may have occurred due to changes in the mobility of chromosomes or proteins that keep the sister chromatids together. The comet assay confirmed the genotoxicity of aluminum sulfate in the kidney tissue of Rhamdia quelen at the three doses of exposure.

  6. DNA damage in the kidney tissue cells of the fish Rhamdia quelen after trophic contamination with aluminum sulfate

    Science.gov (United States)

    Klingelfus, Tatiane; da Costa, Paula Moiana; Scherer, Marcos; Cestari, Marta Margarete

    2015-01-01

    Abstract Even though aluminum is the third most common element present in the earth's crust, information regarding its toxicity remains scarce. It is known that in certain cases, aluminum is neurotoxic, but its effect in other tissues is unknown. The aim of this work was to analyze the genotoxic potential of aluminum sulfate in kidney tissue of the fish Rhamdia quelen after trophic contamination for 60 days. Sixty four fish were subdivided into the following groups: negative control, 5 mg, 50 mg and 500 mg of aluminum sulfate per kg of fish. Samples of the posterior kidney were taken and prepared to obtain mitotic metaphase, as well as the comet assay. The three types of chromosomal abnormalities (CA) found were categorized as chromatid breaks, decondensation of telomeric region, and early separation of sister chromatids. The tests for CA showed that the 5 mg/kg and 50 mg/kg doses of aluminum sulfate had genotoxic potential. Under these treatments, early separation of the sister chromatids was observed more frequently and decondensation of the telomeric region tended to increase in frequency. We suggest that structural changes in the proteins involved in DNA compaction may have led to the decondensation of the telomeric region, making the DNA susceptible to breaks. Moreover, early separation of the sister chromatids may have occurred due to changes in the mobility of chromosomes or proteins that keep the sister chromatids together. The comet assay confirmed the genotoxicity of aluminum sulfate in the kidney tissue of Rhamdia quelen at the three doses of exposure. PMID:26692157

  7. Crecimiento, supervivencia e incidencia de malformaciones óseas en distintos biotipos de Rhamdia quelen durante la larvicultura

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    David R Hernández

    2013-11-01

    Full Text Available Se evaluó el efecto de dos dietas (alimento vivo y dieta seca y dos poblaciones diferentes de Rhamdia quelen de Argentina (área pampeana y nordeste sobre parámetros de crecimiento, supervivencia e incidencia de malformaciones óseas en sus larvas según un diseño factorial 2x2. Al final de la experiencia (20 días pos-eclosión, las deformaciones esqueléticas fueron diversas y afectaron todas las regiones del eje vertebral. En ningún caso estas anomalías incidieron sobre el peso final de las larvas. No obstante, el biotipo nordeste presentó el mayor porcentaje de ejemplares con anomalías esqueléticas (72,3% y menor supervivencia. Las alteraciones más comunes fueron compresiones y fusiones vertebrales, afectando el 22,2 y 19,4% de las larvas, respectivamente. El análisis estadístico mostró que la frecuencia de fusiones no fue afectada por el biotipo o por el tratamiento alimentario. Sin embargo, en el caso de las compresiones vertebrales se observó interacción entre biotipo y dieta. Para este tipo de lesión, con alimento vivo, no se detectaron diferencias entre biotipos, al tiempo que las larvas alimentadas con dieta seca presentaron mayor frecuencia de compresiones en el biotipo nordeste. Estos resultados indican que la incidencia de malformaciones en larvas de R. quelen se encuentra relacionada con ambos factores (biotipo y dieta. La coloración diferencial de cartílagos y huesos en larvas puede convertirse en una herramienta útil para evaluar, precozmente, la incidencia de malformaciones durante el desarrollo temprano de R. quelen.

  8. Larvae occurrences of Rhamdia quelen (Quoy & Gaimard, 1824 (Siluriformes: Heptapteridae in an area under dam influence in the upper Paraná River region, Brazil

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    Evelyn B. da Silva

    2011-06-01

    Full Text Available Rhamdia quelen (Jundiá is a catfish species of great interest to fish producers and researchers due to several favorable characteristics for inclusion in the Brazilian list of commercially raised fish. It has a wide geographical distribution, with occurrences reported from Central Argentina to South Mexico. Studies on the biological aspects of this species have been limited to captive adult fishes that were researched for farming purposes. Research related to ichthyoplankton has provided valuable information on spawning periods and sites and other aspects of breeding for numerous species of fish. This information is crucial for implementing management and protection strategies for these species. The studied floodplain is located in a lotic remnant between the Reservoirs of Itaipu and Engenheiro Sérgio Motta. This area is located inside a protected area (Ilha Grande National Park in the last portion of dam-free land along the Paraná River in the Brazilian territory; however, it is still threatened by the negative effects of the dams. We conducted monthly nocturnal collections during four periods from October to March, 2003 to 2007, in 22 locations along the main channel, secondary lakes and main tributaries of the Paraná River. After collection, the individuals were classified according to the degree of development and the notochord flexion and caudal fin support elements. Data were analyzed using principal component analysis (PCA, ANOVA and Pearson correlation to relate abiotic and larval density data. The increase of capture abundances in the four studied stages suggests that R. quelen is finding conditions that are favorable to its reproduction. The increase in captures can generate questions regarding R. quelen reproductive activity, for instance, whether the species is adapting or is influenced by environmental changes created by the Paraná River dams.Rhamdia quelen (jundiá é uma espécie de bagre que tem despertado grande interesse

  9. Concentrados proteicos vegetais na alimentação de Jundiás (Rhamdia quelen

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    Denize Tyska

    2013-07-01

    Full Text Available O objetivo deste estudo foi avaliar fontes de concentrados proteicos vegetais alternativos em substituição ao farelo de soja sobre o desempenho e composição centesimal de jundiás (Rhamdia quelen e coloração dos filés. Foram utilizadas 20 unidades experimentais, com capacidade de 90L cada uma, onde 400 peixes permaneceram alojados durante 45 dias. Os juvenis de jundiá possuíam 11,5±5,0cm e peso inicial de 10,4±5,5g. Foram avaliadas quatro fontes de concentrados proteicos, sendo eles: dieta controle, tendo o farelo de soja com base proteica; CL: dieta contendo concentrado proteico de crambe + concentrado proteico de linhaça; CLGM: composta por concentrado proteico de crambe + concentrado proteico de linhaça + concentrado proteico de folha de mandioca + concentrado proteico de farelo de girassol e GM: constituída por concentrado proteico de farelo de girassol + concentrado proteico de folha de mandioca. Aos 30 e 45 dias, foram avaliados os parâmetros de crescimento (peso, fator de condição, taxa de crescimento específico, ganho em peso diário e relativo, taxa de eficiência proteica e conversão alimentar aparente e avaliação centesimal do peixe inteiro e filé (umidade, proteína bruta, gordura e cinzas. Além disso, aos 45 dias, foi realizada a avaliação instrumental da cor nos filés. Ao final do período experimental, foram observadas diferenças significativas para os parâmetros de crescimento e avaliação centesimal, sendo que os animais alimentados com as dietas CL apresentaram maior crescimento (13,8cm, taxa de crescimento específico (2,43% dia-1, ganho em peso diário (0,41g dia-1, ganho em peso relativo (108,3% e menor conversão alimentar (1,21, comparado às dietas contendo concentrado proteico de girassol e concentrado proteico de folhas de mandioca. Observou-se também que os animais alimentados com as dietas GM e CLGM apresentaram uma pigmentação amarelada nos filés. Conclui-se que os animais alimentados

  10. BIOLOGÍA REPRODUCTIVA DEL LISO Rhamdia quelen (PISCES: HEPTAPTERIDAE EN EL RÍO SINÚ, COLOMBIA Reproductive Biology of Liso Rhamdia quelen (Pisces: Heptapteridae in the Sinu River, Colombia

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    CHARLES W. OLAYA-NIETO¹

    Full Text Available RESUMEN Se estudió la biología reproductiva del Liso (Rhamdia quelen en el río Sinú. Los peces fueron colectados entre enero y diciembre del 2005, con tallas y pesos entre 15,5 -37 cm de longitud total (LT y 28 -486 g de peso total. Las gónadas se conservaron en solución Gilson, se utilizó la escala de Vazzoler y se estimó proporción sexual, índices de madurez sexual, época de desove, talla media de madurez sexual, diámetro de ovocitos y fecundidad. Se evaluaron 207 hembras, 130 machos y un indiferenciado, con proporción sexual hembra: macho de 1,6:1, diferente a lo esperado. La talla media de madurez fue estimada en 24 cm LT para sexos combinados, el diámetro promedio de los ovocitos fue de 963 µm y la fecundidad promedio fue estimada en 26.305 ovocitos, con ecuación de mejor ajuste F=2960,61 WG 0,88, r=0,94, n=125. El Liso es un pez con tipo de desarrollo ovocitario asincrónico en más de dos grupos, con desoves parciales durante todo el año.ABSTRACT The reproductive biology of Liso (Rhamdia quelen in the Sinu river was studied. The fishes were collected between January and December 2005, with lengths ranging 15,5 -37 cm of total length (TL and 28 - 486 g of total weight. The gonads were placed in Gilson solution, the Vazzoler scale was applicated and sexual proportion, sexual maturity index, spawning season, the length at first maturity, ovocites’s diameter and fecundity were estimated; 207 females, 130 males and 1 undifferentiated were evaluated, with sexual proportion female: male of 1,6:1, different from expected. The length at first maturity was estimated in 24 cm TL for combined sexes; average ovocites’s diameter was 963 mm and average fecundity was estimated in 26.305 ovocites, with better equation F=2960,61 0,88, r=0,94, n=125. The Liso is a fish with asynchronous oocyte development in more than two groups, with partial spawning throughout the year.

  11. INCLUSÃO DE DIFERENTES FONTES DE AMIDO NA DIETA DE JUNDIÁS (Rhamdia quelen): PARÂMETROS METABÓLICOS E BIOQUÍMICOS

    OpenAIRE

    Naglezi de Menezes Lovatto; Fernanda Rodrigues Goulart; Viviani Correia; Cristiano Costenaro Ferreira; Leila Picolli da Silva; João Radünz Neto

    2013-01-01

    This study was conducted to evaluate the influence of ingestion of different sources of starch on enzymatic and metabolic indicators in blood and liver of Rhamdia quelen. The experiment was conducted during 20 days in water recirculation system. Sixty fishes (initial average weight 60g) were used and divided into three treatments composed of the following diets: inclusion of peeled oats, defatted rice bran and control diet, all with 36% crude protein and 3,200 Kcal DE/Kg. There was no signif...

  12. Live and formulated diet evaluation through initial growth and survival of jundiá larvae, Rhamdia quelen Alimento vivo e formulado, crescimento inicial e sobrevivência de pós-larvas de jundiá, Rhamdia quelen

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    Paulo César Falanghe Carneiro

    2003-12-01

    Full Text Available Live diet (LD dependence and the lack of suitable formulated diets (FD are major constraints for the expansion of larviculture of many fish species. The low digestibility and nutritional quality of FD are factors that might explain their failure as a stand-alone starter food. To determine whether FD in combination with LD (zooplankton may efficiently increase larval growth and survival of jundiá (Rhamdia quelen, when compared to fish fed by either types of diet alone, jundiá larvae (5.57 mm; 1.41 mg were initially stocked into 12 10-L aquaria (100 larvae per aquarium. Replicate groups (n=4 were fed ad libitum one of the three diets for 20 (when fed FD or 48 days (when fed LD or the combined diets. Larvae fed FD alone presented significantly lower survival and growth rates as compared to larvae fed LD or a combination of both (co-fed. In addition, co-fed larvae grew better (170 mg in relation to those fed solely with LD (110 mg. Such better performance of combined feeding indicates that most of the required nutrients are in balance when both diet sources are included. More can be learned about fish larvae nutrition by further testing the effect of feeding combined diets, which include zooplankton, than only testing new ingredients or protein sources commonly used in the elaboration of juvenile or adult fish feeds.A larvicultura da maioria das espécies de peixes enfrenta o desafio da dependência do alimento vivo (AL e da falta de dietas formuladas (DF que atendam plenamente às necessidades das larvas. A baixa digestibilidade e a qualidade nutricional das DFs são alguns dos fatores que explicam o insucesso quando as larvas recebem apenas FD. Para avaliar o efeito da combinação da DF com o AL no crescimento e na sobrevivência de larvas de jundiá (Rhamdia quelen, comparando com o uso separado da DF ou do AL, larvas recém eclodidas (5,57 mm; 1,41 mg foram estocadas inicialmente em 12 aquários de 10 L (100 larvas por aquário. Quatro r

  13. Diferentes fontes protéicas para a alimentação do jundiá (Rhamdia quelen Different protein sources for jundiá (Rhamdia quelen feeding

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    Rafael Lazzari

    2006-02-01

    Full Text Available O objetivo deste trabalho foi verificar o crescimento e a composição de filés de juvenis de jundiá (Rhamdia quelen alimentados com diferentes fontes protéicas, durante 60 dias. Utilizaram-se 540 peixes (peso médio inicial=15,00±0,62g, comprimento total inicial=11,98±0,35cm distribuídos ao acaso em 18 caixas de 280L (30 peixes/caixa em sistema de recirculação de água. Testaram-se seis tratamentos (com três repetições: CL (farinha de carne e ossos + levedura, SL (farelo de soja + levedura, S (somente farelo de soja, CS (farinha de carne e ossos + farelo de soja, PL (farinha de peixe + levedura e PS (farinha de peixe + farelo de soja. Foram estimados parâmetros de desempenho (peso, comprimento total, fator de condição, taxa de crescimento específico, taxa de eficiência protéica aos 30 e 60 dias, composição centesimal e taxas de deposição de proteína e gordura nos filés ao final do experimento. Os parâmetros peso, comprimento total e taxa de crescimento específico foram superiores nos tratamentos compostos pela combinação das farinhas de origem animal (carne e ossos e peixe com farelo de soja. A quantidade de gordura e proteína depositada no filé dos peixes também foi superior nestes tratamentos (CS e PS. Os rendimentos de carcaça e filé não diferiram entre os tratamentos. A combinação das farinhas de carne e ossos e de peixes com farelo de soja possibilita bom crescimento e maior deposição de proteína e gordura nos filés de juvenis de jundiá.This study aimed at verifing the growth and fillet composition of jundiá juveniles (Rhamdia quelen fed with different protein sources, for 60 days. It was used 540 fish (initial weight=15.00±0.62g, initial length=11.98±0.35cm at random distributed in 18 tanks of 280L (30 fish/tank in a water re-use system. 6 treatments were tested (with 3 repetitions: CL (meat and bone meal + yeast, SL (soybean meal + yeast, S (only soybean meal, CS (meat and bone meal + soybean

  14. Eugenol as anesthetic for silver catfish (Rhamdia voulezi) with different weight
    Eugenol como anestésico para jundiá (Rhamdia voulezi) em diferentes pesos

    OpenAIRE

    Wilson Rogerio Boscolo; Altevir Signor; Fabio Bittencourt; Dacley Hertes Neu; Odair Diemer; Aldi Feiden

    2012-01-01

    The study aimed to find the better concentration of eugenol for anesthesia of silver catfish (Rhamdia voulezi) with different weights. Were used 240 catfish distributed in randomized blocks in factorial scheme (5x4) total 20 treatments, in others words, five different weights: 32,5; 75; 150; 300 e 450g and four eugenol concentrations: 50, 75, 100 e 125 mg.l-1. For each treatment were used 12 fish randomly chosen and exposed individually for each concentration. After of anesthesia the fish wer...

  15. INCLUSÃO DE DIFERENTES FONTES DE AMIDO NA DIETA DE JUNDIÁS (Rhamdia quelen: PARÂMETROS METABÓLICOS E BIOQUÍMICOS

    Directory of Open Access Journals (Sweden)

    Naglezi de Menezes Lovatto

    2013-09-01

    Full Text Available This study was conducted to evaluate the influence of ingestion of different sources of starch on enzymatic and metabolic indicators in blood and liver of Rhamdia quelen. The experiment was conducted during 20 days in water recirculation system. Sixty fishes (initial average weight 60g were used and divided into three treatments composed of the following diets: inclusion of peeled oats, defatted rice bran and control diet, all with 36% crude protein and 3,200 Kcal DE/Kg. There was no significant difference in growth parameters. The analysis demonstrated a reduction in liver glycogen content for the treatment with oats; however, no apparent changes in the activity of the enzymes alanine-aminotransferase and glucose-6-phosphatase were observed, raising the possibility that the fishes did not use gluconeogenesis. The blood analysis, showed no significant differences in the determinations of total cholesterol, HDL cholesterol and albumin; however, serum levels of total protein were higher for treatment with defatted rice bran, and triglyceride levels were lower in animals that received diet containing oat as starch source. It can be concluded that Rhamdia quelen is able to metabolize various sources of carbohydrate, without the need to use either diet or body protein as an energy source.

  16. Efeito de níveis crescentes de levedura de álcool em rações contendo fígado bovino sobre a performance de larvas de jundiá (Rhamdia quelen Effect of crescent levels of yeast of alcohol used in rations containing bovine liver on larvae of (Rhamdia quelen performance

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    Rosamari Piaia

    1997-06-01

    Full Text Available O presente trabalho foi realizado com a finalidade de avaliar o efeito de diferentes níveis de levedura sobre o desempenho inicial de larvas de Rhamdia quelen durante as trêss primeiras semanas de vida. Um total de 3000 larvas foram distribuídas em quinze grupos, em um sistema de criação com reutilização de água, termoregulada. Utilizou-se cinco tratamentos com níveis crescentes de levedura (T1 - 50%, T2 - 60%, T3 - 70%, T4 - 80% e T5 - 90% em substituição ao fígado bovino fresco. Os níveis de proteína bruta (PB e energia digestível variaram de 41,36 a 26,56% de PB e 3197 a 2789kcal ED/kg. A granulometria dos alimentos testados foi de 100 a 200mi, 200 a 400mi e 400 a 600mi para as três semanas experimentais, respectivamente. O alimento foi fornecido a vontade entre 8 e 20 horas. Dentro dos limites de proteína estudados houve efeito positivo de níveis mais elevados de PB sobre os comprimentos total e padrão e peso individual. Porém houve um efeito negativo sobre a sobrevivência das larvas de jundiá Rhamdia quelen.The present study was developed with the purpose of evaluating the effects of different protein levels in initial performance of Rhamdia quelen larvae during the first three weeks of life. The sample contained 3000 larvae distributed in fifteen groups, maintained in controlled conditions of culture and utilizing termoregulatory water re-use system. Five crescent levels of yeast (T1 50%, T2 60%, T3 70%, T4 80% and T5 90% were used in rations in substituition of bovine liver. Levels of crude protein and digestible energy varied 41,36% to 26,56% CP and from 3197 to 2789 kcal ED/kg. The granulometry of tested food was of 100 a 200mu, 200 a 400mu and 400 a 600mu for three experimental weeks respectively. Food distribution provided at ease between 8 Am and 8 Pm. Within the net protein used in this research, there was found a positive effect for the highest protein levels for total, standard lenght and individual weight

  17. Larvicultura de Rhamdia quelen (Pisces, Pimelodidae con proteína vegetal y animal, suplementadas con plancton

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    Germán Castañeda A.

    2011-12-01

    Full Text Available Objetivo. Determinar el efecto de dietas con diferentes proporciones de fuentes proteicas animales y vegetales suplementadas con plancton sobre el crecimiento y sobrevivencia en larvas de Rhamdia quelen. Materiales y métodos. Fueron formuladas dos dietas experimentales, dieta-1 con 70% de proteína de origen vegetal (torta de soya y dieta-2 con 70% de proteína proveniente de animales (corazón bovino yharina de pescado; se utilizaron cuatro protocolos de alimentación (cinco réplicas por tratamiento: dieta-1 + plancton filtrado en tamiz de 50-200 micras (T1, dieta-2 + plancton filtrado en tamiz de 50-200 micras (T2, dieta-1 (T3, dieta-2 (T4. Las larvas fueron manejadas a una densidad de 20 animales L-1 suministrando alimentohasta aparente saciedad diariamente a las 07:00, 11:00, 16:00 y 21:00 horas; la suplementación con plancton se realizó a las 11:00 y 21:00 horas. Resultados. Entre T1 y T2 no se observaron diferencias estadísticas (p>0.05 en peso final (21.89 ± 15.17mg vs 20.37 ± 10.37mg, longitud total (13.41±2.34mm vs 13.39±1.99 mm, factor de condición (K (0.80±0.13 vs 0.78±0.13 y sobrevivencia (46.6±2.68% vs36.0±7.41%; las diferencias entre T3 y T4 tampoco fueron significativas (3.35±1.40 mg vs 2.98±1.48 mg; 7.54±0.91mm vs 7.33±0.96mm; 0.75±0.13 vs 0.71±0.12; 33.6±9.07% vs 24.8±6.76%, respectivamente; hubo diferencias significativas (p<0.05 entre los grupos suplementados con plancton y los alimentados solo con ración. Conclusiones. La suplementación con plancton fue más efectiva que elofrecimiento solo de ración. La inclusión del 62.9% de torta de soya en la formulación (T1, aparentemente no afectó el crecimiento ni la sobrevivencia.

  18. ALTERAÇÕES METABÓLICAS E HEMATOLÓGICAS EM JUNDIÁS (Rhamdia quelen) ALIMENTADOS COM RAÇÕES CONTENDO AFLATOXINAS

    OpenAIRE

    Milene Braga da Fonseca; Rafael Lazzari; Paulo Rodinei Soares Lopes; João Radünz Neto; Vânia Lucia Pimentel Vieira; Charlene Cavalheiro Menezes

    2006-01-01

    A presente pesquisa teve por objetivo verificar a influência de diferentes níveis de aflatoxinas sobre alguns parâmetros metabólicos e hematológicos em alevinos de jundiá (Rhamdia quelen), utilizando-se peixes com peso inicial de 3,0 gramas, criados em sistema de recirculação de água, durante 45 dias, com quatro tratamentos experimentais, sendo um controle e três níveis de aflatoxinas: 41, 90 e204 ppb de aflatoxinas/kg de ração. Os resultados obtidos demonstraram redução nos valores de hemató...

  19. Feeding activity and influence of intraspecific competition on zooplankton communities by jundiá (Rhamdia quelen Quoy and Gaimard, 1824) in laboratory.

    Science.gov (United States)

    Brandão-Gonçalves, L; Sebastien, N Y

    2013-11-01

    Experiments were designed to determine if the juvenile forms of jundia (Rhamdia quelen) exhibit selectivity feeding on zooplanktonic organisms and the influence of intraspecific competition on the diet. The fish were maintained in 5000-L containers, and after seven days from birth, they were acclimated in experimental units with different densities and supplied with water containing plankton (natural), where they remained for 24 h in the environment with food. In the first seven days at densities of 5 and 10 fish.L-1, the fish selected the Rotifera more intensely, and at densities of 20 and 40 fish.L-1, the Cladocera were more selected. At 14 days of age, the Cladocera were the most selected for all densities of fish. At 21 days of age, the juvenile forms of jundia maintained a preference for Cladocera, and there was an increase in the capture of Copepoda at a density of 40 fish.L-1. At 28 days of age, the Cladocera were the most captured at densities of 5 to 20 fish.L-1, and the consumption of copepods increased at a density of 40 fish.L-1. The juvenile forms of Rhamdia quelen showed a high feeding preference for Cladocera at all the ages tested (7, 14, 21 and 28 days), but the density of fish per litre influenced the capture of the preferential food. This study showed that the juvenile forms of jundia had a more rapid response to the presence of food at densities of more than 10 fish.L-1, and that at higher densities the fish decreased interactions of territorial defense and formed schools, which allowed the foraging of zooplanktonic organisms, larger in size, more agile and of greater energy gain, such as the Cladocera and copepods. PMID:24789392

  20. ALTERAÇÕES METABÓLICAS E HEMATOLÓGICAS EM JUNDIÁS (Rhamdia quelen ALIMENTADOS COM RAÇÕES CONTENDO AFLATOXINAS

    Directory of Open Access Journals (Sweden)

    Milene Braga da Fonseca

    2006-10-01

    Full Text Available A presente pesquisa teve por objetivo verificar a influência de diferentes níveis de aflatoxinas sobre alguns parâmetros metabólicos e hematológicos em alevinos de jundiá (Rhamdia quelen, utilizando-se peixes com peso inicial de 3,0 gramas, criados em sistema de recirculação de água, durante 45 dias, com quatro tratamentos experimentais, sendo um controle e três níveis de aflatoxinas: 41, 90 e204 ppb de aflatoxinas/kg de ração. Os resultados obtidos demonstraram redução nos valores de hematócrito, hemoglobina e glicose sangüínea dos peixes, proporcionalmente aos níveis crescentes de incorporação de aflatoxinas na dieta. Quanto aos metabólitos no tecido hepático e muscular, a principal alteração foi a redução nos teores de proteína muscular e hepática em resposta aos níveis adicionados. Conclui-se que, para os níveis de aflatoxinas testados, ocorre diminuição dos parâmetros hematológicos em alevinos de jundiá. Ocorre também utilização da proteína do músculo e do fígado pelos peixes,sendo que as outras alterações metabólicas podem ser consideradas adaptações do peixe em relação à intoxicação. PALAVRAS-CHAVE: Aflatoxinas, hematócrito, intoxicação, metabólitos, Rhamdia quelen.

  1. Eugenol as anesthetic for silver catfish (Rhamdia voulezi with different weightEugenol como anestésico para jundiá (Rhamdia voulezi em diferentes pesos

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    Wilson Rogerio Boscolo

    2012-08-01

    Full Text Available The study aimed to find the better concentration of eugenol for anesthesia of silver catfish (Rhamdia voulezi with different weights. Were used 240 catfish distributed in randomized blocks in factorial scheme (5x4 total 20 treatments, in others words, five different weights: 32,5; 75; 150; 300 e 450g and four eugenol concentrations: 50, 75, 100 e 125 mg.l-1. For each treatment were used 12 fish randomly chosen and exposed individually for each concentration. After of anesthesia the fish were transferred for net-cage with 0,7m3, being fed and observed by 96 hours for monitoring of mortality. The eugenol was efficient for anesthesia in silver catfish all concentrations and weights and after of 96 hours no mortality have been verified. At these experimental conditions the best concentration of eugenol for anesthetic inducing and recuperation of silver catfish with weight varying from 32,5 and 450 g is 50 mg.l-1. O presente estudo teve como objetivo encontrar a melhor dose de eugenol para a anestesia do jundiá (Rhamdia voulezi em diferentes classes de peso. Foram utilizados 240 jundiás distribuídos em delineamento experimental em blocos em esquema fatorial (5 x 4 totalizando 20 tratamentos, ou seja, cinco diferentes classes de peso: 32,5; 75; 150; 300 e 450g e quatro concentrações de eugenol (50, 75, 100 e 125 mg.l-1. Para cada tratamento foram utilizados 12 peixes escolhidos aleatoriamente (n = 12 e expostos individualmente para cada concentração. Após o procedimento de anestesia os peixes foram transferidos para tanques-rede com 0,7m3, onde receberam alimentação e ficaram em observação durante 96 horas para o monitoramento da mortalidade. O eugenol foi eficiente para a anestesia em jundiás nas diferentes concentrações analisadas e nos distintos pesos, e após 96 horas de acompanhamento à recuperação anestésica, não foram verificadas mortalidades dos animais. Nas condições deste experimento a melhor concentração de eugenol

  2. Análise de diferentes dosagens de hormônio na ração, para definição de um protocolo de feminilização do jundiá Rhamdia quelen

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    Hilton Amaral Junior

    2008-12-01

    Full Text Available Con el objetivo de aumentar el número de hembras en los lotes de Jundiás Rhamdia quelen utilizados para el cultivo ya que tienen un crecimiento superior a la de los machos de la especie, las larvas de jundiás de un día de vida, fueron sometidos a una dieta con raciones que contengan incorporadas determinadas cantidades de la hormona 17 beta estradiol, durante un período de 30 días. Los mejores resultados a la inversión d sexo se presentaron en los animales tratados con dosis de 100 mg de la hormona por kilogramo de dieta.

  3. Efeito do chumbo sobre a fertilização artificial e incubação de ovos de jundiá cinza (Rhamdia quelen = The effect of lead on artificial fertilization and incubation of gray jundiá (Rhamdia quelen eggs

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    Cleonice Cristina Hilbig

    2008-04-01

    Full Text Available A poluição no meio aquático afeta o potencial reprodutivo de várias espécies de peixes. Diante disso, o presente experimento foi conduzido com o objetivo de avaliar os efeitos causados pela contaminação da água utilizada no processo de fertilização artificial e incubação inicial de ovos de jundiá cinza (Rhamdia quelen por chumbo. Foram realizados dois ensaios, os quais foram distribuídos em delineamento experimental inteiramente casualizado, compostos por seis tratamentos, com quatro e seis repetições, para o primeiro eo segundo ensaio, respectivamente. O primeiro ensaio consistiu na contaminação da água utilizada no processo de fertilização artificial, com os níveis de 0,00; 0,01; 0,02; 0,03; 0,04 e0,05 mg de PB L-1, e incubação dos ovos em água limpa. O segundo ensaio utilizou os níveis de contaminação de 0,00; 0,15; 0,30; 0,45; 0,60 e 0,75 mg de PB L -1 de água utilizada nos processos de fertilização e de incubação dos ovos. Observou-se que apenas no segundo ensaio houve efeito (p Aquatic environmental pollution affects the reproductive potential of various species of fish. The present experiment was carried out to evaluate the effect of lead contamination of the water used in artificial fertilization and initial egg incubation of gray jundiá (Rhamdia quelen. Two assays were carried out distributed inrandomly experimental designs, composed by six treatments, with four and six replications, for the first and second assays, respectively. The first assay consisted of the contamination of artificial fertilization water with 0.00; 0.01; 0.02; 0.03; 0.04 and 0.05 mg of Pb L-1 and egg incubation in pure water. The second assay consisted of the contamination of artificial fertilization water and egg incubation water with 0.00; 0.15; 0.30; 0.45; 0.60 and 0.75 mg ofPb L-1. Only the second assay was affected (p < 0.05 by the treatments, showing a “plateau” tendency until 0.25 mg of Pb L-1. A linear reduction of the

  4. Ammonia excretion at different life stages of silver catfish=Excreção de amônia em diferentes estágios de vida do jundiá

    OpenAIRE

    Vania Lúcia Loro; Alexssandro Geferson Becker; Neiva Braun; Luciano de Oliveira Garcia; Bernardo Baldisserotto

    2012-01-01

    This study examined ammonia excretion at different life stages (eggs, larvae and juveniles) in silver catfish (Rhamdia quelen) and determined the influence of fasting time on ammonia excretion. Eggs and larvae were collected from incubators at different times after fecundation and placed in chambers. Juveniles were separated into two weight classes (2-50 g and 150-320 g) and placed in individual chambers after feeding. Water was collected from each chamber to determine ammonia excretion. Ammo...

  5. Alimentação de fêmeas de jundiá com fontes lipídicas e sua relação com o desenvolvimento embrionário e larval Use of lipid sources on feeding jundiá (Rhamdia quelen and its relation with embryo and larval development

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    Jorge Erick Garcia Parra

    2008-10-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência da alimentação de fêmeas de jundiá com diferentes fontes lipídicas no desenvolvimento embrionário e larval. Foram utilizadas 12 fêmeas de jundiá (peso inicial de 500g, distribuídas ao acaso em três tanques-rede (1m³, alimentadas durante 10 semanas. Foram utilizados três dietas contendo como fontes lipídicas: banha suína (BS, óleo de girassol (OG, óleo de canola (OC. No momento da eclosão, foram coletadas nas incubadoras amostras de dez (10 larvas. Uma amostra de pós-larvas foi coletada às 12, 24, 36 e 48 horas pós-eclosão para medição. Mais três amostras de pós-larvas de cada incubadora foram capturadas e criadas durante 14 dias (30 larvas L-1. O desempenho das fêmeas e o desenvolvimento larval não foram afetados pelas fontes lipídicas testadas. Conclui-se que a banha suína é tão eficiente quanto óleo de girassol e canola como fontes lipídicas para fêmeas reprodutoras de jundiá e proporcionam bom desenvolvimento embrionário e larval.The aim of this study was to evaluate the efficiency of feeding female South America catfish Rhamdia quelen with different lipids sources and their influence in embryo and larval stage. Twelve (12 female catfishes initial weight of 500g, distributed randomly among three (3 net-tanks and fed for ten (10 weeks were used. The female fishes were fed with three (3 different types of experimental diet containing lipid sources: Swine fat (BS, sunflower oil (OG canola oil (OC. At hatch time, 10 larvae were collected from each net tank. One post larvae sample was collected at 12, 24, 36, 48 hours after hatch to verify measurement. Three samples were captured and raised in reuse water system during fourteen days (30 larvae L-1. The female reproductive performance and the larval development were not affected by the lipid sources tested. The data revealed that swine fat is as good as sunflower and canola oil as lipid sources for reproductive

  6. Proximate composition, microbiological and sensory of jundiá (Rhamdia quelen submitted to the smoking process/ Composição centesimal, microbiológica e sensorial do jundiá (Rhamdia quelen submetido ao processo de defumação

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    Wilson Rogério Boscolo

    Full Text Available The objective of the experiment was to evaluate the proximate composition, microbiological and sensory of jundia (Rhamdia quelen of whole eviscerated and fillet submitted to the process of hot smoking. Were used 22 jundias, separated into three categories: A: small whole fish (122.25 ± 25.39g; B: headless large whole fish (358.4 ± 50.98g and C: fillet (126.92 ± 12.48g. Samples of each category were used for microbiological analysis of raw material in natura. The remainder was submitted to dry salting with 25% of concentration for 60 minutes, after were realized the process of hot smoking. Wasn’t observed significative differences (P ? 0.05 between the parameters of crude protein, ether extract and moisture of smoked products. With the sensory analysis, was observed that the fillet had higher intensity of salt, differing (P ? 0.05 from the whole smoked fish. There were no significative differences (P ? 0.05 on the color and acceptance of products smoked. With the flavor, the tasters revealed preference (P ? 00.5 to small whole smoked fish. The result of the count of microorganisms was low, indicating that the products were under the standards established by law for consumption. It is concluded that the hot smoking process applied in different ways to the fish (small whole fish, headless large whole fish and fillet does not affect the proximate composition of the final product. There was a significant preference for small whole fish smoked in the case of flavors and for headless large whole fish smoked in the case of acceptance, smoked jundia can being consumed with security for a period of 21 days of storage at temperature of ± 5ºC beyond to add value to smoked fish.O objetivo do presente trabalho foi avaliar a composição centesimal, microbiológica, e sensorial do jundiá (Rhamdia quelen inteiro eviscerado e filetado submetido ao processo de defumação a quente. Foram utilizados 22 jundiás, separados em três categorias: A: peixe

  7. Criação de larvas de jundiá (Rhamdia quelen alimentadas com rações granuladas contendo fígados ou hidrolisados - DOI: 10.4025/actascianimsci.v26i4.1718 Rearing of “jundiá” (Rhamdia quelen larvae fed with diets containing liver or hydrolysates - DOI: 10.4025/actascianimsci.v26i4.1718

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    Tanise dos Santos Medeiros

    2004-04-01

    Full Text Available O jundiá (Rhamdia quelen é uma espécie nativa da região Sul do Brasil, que apresenta rápido crescimento, rusticidade, carne de bom sabor e grande potencial para criação intensiva. No presente trabalho, objetivou-se verificar o uso de hidrolisados de peixe e de fígado de aves na criação de larvas de jundiá. Foram realizados dois experimentos, utilizando-se 3200 larvas em cada. No primeiro experimento, testou-se 4 dietas contendo como base levedura de cana e fígado bovino ou de aves. O tratamento composto por fígado de aves cru resultou em maiores valores de comprimento total, peso médio individual, produto peso versus sobrevivência e taxa de crescimento específico. No segundo experimento, foram testados os dois melhores tratamentos do experimento anterior comparando-os com alimentos preparados com hidrolisados de peixes e de fígado de aves, em substituição ao fígado. As maiores taxas de sobrevivência foram encontradas nos alimentos à base de fígado bovino e de aves. Em relação aos demais parâmetros analisados, como no primeiro experimento, os resultados do alimento preparado à base de fígado de aves cru foram significativamente superiores aos demais.The “jundiá” (Rhamdia quelen it is native specie of the Southern Brazil, that present fast growth, rusticity, meat of good flavor and great potential for intensive fish farming. This study aimed verify the use of fish hydrolysates and broilers liver on the jundiá’s larvae rearing. Two experiments were accomplished; being used 3200 larvae in each. In the first experiment it was tested four diets containing sugar canne yeast and bovine or broilers livers. The treatment composed by raw broiler liver resulted in higher values of total length, standard weight, product weight versus survival and specific growth rate. In the second experiment the two better treatments of the previous experiment were tested comparing them with foods prepared with fish hydrolysates and

  8. Avaliação do jundiá (Rhamdia quelen em diferentes sistemas de cultivo para a região do litoral centro norte de Santa Catarina, Brazil

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    Hilton Amaral Junior

    2008-12-01

    Full Text Available El bagre (Rhamdia quelen, un pez nativo habitante de la mayoría de los ríos, lagos y lagunas de Santa Catarina, y que pertenecen a la orden Siluriformes, familia Pimelodidae, género Rhandia. El estudio tiene como objetivo evaluar el desempeño de este pez nativo en los sistemas más utilizados en Santa Catarina. El experimento se llevó a cabo en el ámbito del Campo Experimental de Piscicultura de Camboriú CEPC-EPAGRI, cuando 11 tanques excavados fueron utilizados, tres en representación de un monocultivo de bagre con una densidad de 1,5 p/m2, tres en representación de un bicultivo de bagre y tilapia con una densidad de 3p/m2, tres en representación de un policultivo de la carpa, tilapia y "jundiás" con una densidad de 3p/m2, y en dos tanques excavados fueron instalados seis tanques redes, en representación de las culturas de alta densidad de 50 y 100 p/m3.

  9. Anesthesia of silver catfish with eugenol: time of induction, cortisol response and sensory analysis of fillet Anestesia de jundiás com eugenol: tempo de indução, resposta ao cortisol e análise sensorial do filé

    OpenAIRE

    Mauro Alves da Cunha; Carla Cristina Zeppenfeld; Luciano de Oliveira Garcia; Vania Lucia Loro; Milene Braga da Fonseca; Tatiana Emanuelli; Ana Paula Lima Veeck; Carlos Eduardo Copatti; Bernardo Baldisserotto

    2010-01-01

    The aim of this study was to identify the time of anesthetic induction and recovery of silver catfish (Rhamdia quelen) exposed to eugenol. It was also determined the efficacy of the anesthetic as a stress reducing agent and performed a sensory analysis of the fillets from fish exposed to this substance. The silver catfish were exposed to air for 1min to carry out biometry, and blood was collected at 0, 1 and 4 hours later. Eugenol can be used in the range of 20-50mg L-1 for anesthetic inducti...

  10. Desempenho do jundiá, Rhamdia quelen, e do dourado, Salminus brasiliensis, em viveiros de terra na região sul do Brasil - DOI: 10.4025/actascianimsci.v26i3.1806 Performance of Jundiá, Rhamdia quelen, and Dourado, Salminus brasiliensis, in earth ponds of southern Brazil - DOI: 10.4025/actascianimsci.v26i3.1806

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    Fábio Mazzoti Santamaria

    2004-04-01

    Full Text Available Este estudo teve como objetivo principal avaliar o potencial do jundiá, Rhamdia quelen, e do dourado, Salminus brasiliensis, para exploração em aqüicultura no Sul do Brasil. Para tanto, a velocidade de crescimento, conversão alimentar, diferença de crescimento entre machos e fêmeas, resistência ao manejo e sobrevivência destas espécies foram avaliadas. Alevinos destas duas espécies foram estocados em viveiros de terra, em dois municípios de Santa Catarina: Santo Amaro da Imperatriz (SA e São Carlos (SC. Estes foram alimentados com dietas comerciais, duas vezes ao dia, até a saciedade aparente. Após 365 (jundiás e 605 (dourados dias, os jundiás apresentaram um ganho em peso diário (GPD de 1,00g e 1,97g e conversão alimentar de 1,85 e 1,78, em SA e SC, respectivamente, enquanto os dourados um GPD de 0,46g e 0,51g e conversão de 3,52 e 3,82, em SA e SC, respectivamente. Em SA, foram detectadas diferenças em GPD, fator de condição e índice gônado-somático entre machos e fêmeas. Fatores como densidade de estocagem, tamanho de viveiro e proporção sexual são discutidos para as duas espécies.The present study aimed to evaluate the aquaculture potential in Southern Brazil for two species of freshwater fish: dourado, Salminus brasiliensis and jundiá, Rhamdia quelen. Growth, feed conversion, growth differences between males and females, resistance to handling and survival rate were evaluated. Fingerlings of the two species were stocked in earthen ponds at two different locations in the State of Santa Catarina: Santo Amaro da Imperatriz (SA and São Carlos (SC. Fish were fed commercial diets twice a day to apparent satiation. After 365 days (jundiá and 605 (dourado days, jundiá presented a daily weight gain (DWG of 1.00 and 1.97g and feed conversion of 1.85 and 1.78 at SA and SC, respectively, whereas for dourado, the DWG was 0.46 and 0.51 and feed conversion was 3.52 and 3.82 at SA and SC, respectively. At SA, differences

  11. Resposta fisiológica à infestação experimental com Ichthyophthirius multifiliis (Fouquet, 1876 em alevinos de jundiá (Rhamdia quelen Quoy e Gaimard, 1824 previamente alimentados com diferentes fontes lipídicas Physiological response of experimental challenge of Ichthyophthirius multifiliis (Fouquet, 1876 in jundiá (Rhamdia quelen Quoy and Gaimard, 1824 fingerlings rather fed with different lipid sources

    Directory of Open Access Journals (Sweden)

    Rodrigo J. Vargas

    2008-06-01

    Full Text Available Um fator limitante nas primeiras fases do cultivo do jundiá (Rhamdia quelen Quoy e Gaimard, 1824 é a presença da ictioftiríase. Os ácidos graxos poliinsaturados (PUFAs presentes na dieta possuem uma potente atividade imuno-moduladora, e esta atividade depende da espécie analisada e da quantidade/qualidade dos PUFAs da dieta. O objetivo do presente estudo foi avaliar a resposta fisiológica de alevinos de jundiá, alimentados com diferentes fontes de ácidos graxos, quando parasitados pelo protozoário Ichthyophthirius multifiliis (Fouquet, 1876. Alevinos de jundiá (7,6±0,8g alimentados durante sete semanas com cinco dietas contendo diferentes fontes lipídicas: óleos de peixe, linhaça e milho foram expostos ao protozoário. No quinto dia a presença da doença foi detectada. Amostras de sangue foram coletadas e sobrevivência e graus de infestação foram estimados. As diferentes dietas afetaram a sobrevivência dos alevinos, sendo que alevinos que consumiram dietas com óleo de peixe apresentaram uma maior sobrevivência. O hematócrito e o grau de infestação não apresentaram diferença estatística significativa entre os tratamentos, entretanto a contagem diferencial dos leucócitos registrou diferença. Os resultados mostraram pela primeira vez no jundiá que, diferentes fontes lipídicas produzem respostas fisiológicas diversas, podendo representar uma alternativa ao tratamento da doença. Novos estudos deverão ser realizados no sentido de aprofundar o conhecimento aqui gerado.A limiting factor in the early stage of the jundiá (Rhamdia quelen Quoy; Gaimard, 1824 culture is the presence of diseases such as ichthyophthiriasis or "Ich". Dietary polyunsaturated fatty acids (PUFAs have a potent immunomodulatory activity; and this activity depend of the fish species, quantity/quality of the dietary PUFAS. The aim of this study was evaluate the physiologic response ofjundiá, rather fed diets with different sources of fatty acids

  12. Efeito do chumbo sobre a fertilização artificial e incubação de ovos de jundiá cinza (Rhamdia quelen - DOI: 10.4025/actascianimsci.v30i2.4703 The effect of lead on artificial fertilization and incubation of gray jundiá (Rhamdia quelen eggs - DOI: 10.4025/actascianimsci.v30i2.4703

    Directory of Open Access Journals (Sweden)

    Diego Mendes Baggio

    2008-08-01

    Full Text Available A poluição no meio aquático afeta o potencial reprodutivo de várias espécies de peixes. Diante disso, o presente experimento foi conduzido com o objetivo de avaliar os efeitos causados pela contaminação da água utilizada no processo de fertilização artificial e incubação inicial de ovos de jundiá cinza (Rhamdia quelen por chumbo. Foram realizados dois ensaios, os quais foram distribuídos em delineamento experimental inteiramente casualizado, compostos por seis tratamentos, com quatro e seis repetições, para o primeiro e o segundo ensaio, respectivamente. O primeiro ensaio consistiu na contaminação da água utilizada no processo de fertilização artificial, com os níveis de 0,00; 0,01; 0,02; 0,03; 0,04 e 0,05 mg de Pb.L-1, e incubação dos ovos em água limpa. O segundo ensaio utilizou os níveis de contaminação de 0,00; 0,15; 0,30; 0,45; 0,60 e 0,75 mg de Pb.L-1de água utilizada nos processos de fertilização e de incubação dos ovos. Observou-se que apenas no segundo ensaio houve efeito (p -1, a partir do qual se verificou redução das taxas de fertilização de forma linear. Conclui-se que níveis de chumbo superiores a 0,25 mg de Pb.L-1causaram efeito deletério sobre a fertilização artificial do jundiá cinza.Aquatic environmental pollution affects the reproductive potential of various species of fish. The present experiment was carried out to evaluate the effect of lead contamination of the water used in artificial fertilization and initial egg incubation of gray jundiá (Rhamdia quelen. Two assays were carried out distributed in randomly experimental designs, composed by six treatments, with four and six replications, for the first and second assays, respectively. The first assay consisted of the contamination of artificial fertilization water with 0.00; 0.01; 0.02; 0.03; 0.04 and 0.05 mg of Pb L-1 and egg incubation in pure water. The second assay consisted of the contamination of artificial fertilization

  13. Reappraisal of Rhamdia branneri Haseman, 1911 and R. voulezi Haseman, 1911 (Siluriformes: Heptapteridae) from the rio Iguaçu with notes on their morphometry and karyotype

    OpenAIRE

    Julio Cesar Garavello; Oscar Akio Shibatta

    2016-01-01

    ABSTRACT The species Rhamdia branneri Haseman, 1911 and the subspecies Rhamdia branneri voulezi Haseman, 1911 from rio Iguaçu are currently recognized as synonyms of Rhamdia quelen (Quoy & Gaimard, 1824). However, recent karyotype and ecomorphology studies distinguish R. branneri and R. voulezi as different species. Examination of Rhamdia populations from rio Iguaçu, including type specimens, together with other congeners from rio Tibagi, allowed to properly reexamine the situation of these H...

  14. Effects of starvation, refeeding, and insulin on energy-linked metabolic processes in catfish (Rhamdia hilarii) adapted to a carbohydrate-rich diet

    International Nuclear Information System (INIS)

    The effects of starvation and of a short period of refeeding on energy-linked metabolic processes, as well as the effects of insulin administration, were investigated in an omnivorous fish (catfish, Rhamdia hilarii) previously adapted to a carbohydrate-rich diet. Following food deprivation blood sugar levels declined progressively to about 50% of fed values after 30 days. During the same period plasma free fatty acid (FFA) concentration increased twofold. Starvation resulted in reduced concentrations of lipid and glycogen in the liver and of glycogen, lipid, and protein in white muscle. However, taking into account the initial and final concentrations of tissue constituents, the liver weight, and the large fractions of body weight represented by muscle, it could be estimated that most of the energy utilized during starvation derived from the catabolism of muscle lipid and protein. Refeeding starved fishes for 48 hr induced several-fold increases in the rates of in vivo and in vitro incorporation of [14C]glucose into liver and muscle lipid and of [14C]glycine into liver and muscle protein. Incorporation of [14C]glucose into liver glycogen was also increased. However; refeeding did not affect the incorporation of labeled glucose into muscle glycogen, neither in vivo nor in vitro. Administration of pharmacological doses of insulin to normally fed catfishes resulted in marked increases in the in vivo incorporation of 14C from glucose into lipid and protein in both liver and muscle. In contrast, labeled glucose incorporation into muscle glycogen was not affected by insulin and label incorporation into liver glycogen was actually lower than that in noninjected controls

  15. Effects of starvation, refeeding, and insulin on energy-linked metabolic processes in catfish (Rhamdia hilarii) adapted to a carbohydrate-rich diet

    Energy Technology Data Exchange (ETDEWEB)

    Machado, C.R.; Garofalo, M.A.; Roselino, J.E.; Kettelhut, I.C.; Migliorini, R.H.

    1988-09-01

    The effects of starvation and of a short period of refeeding on energy-linked metabolic processes, as well as the effects of insulin administration, were investigated in an omnivorous fish (catfish, Rhamdia hilarii) previously adapted to a carbohydrate-rich diet. Following food deprivation blood sugar levels declined progressively to about 50% of fed values after 30 days. During the same period plasma free fatty acid (FFA) concentration increased twofold. Starvation resulted in reduced concentrations of lipid and glycogen in the liver and of glycogen, lipid, and protein in white muscle. However, taking into account the initial and final concentrations of tissue constituents, the liver weight, and the large fractions of body weight represented by muscle, it could be estimated that most of the energy utilized during starvation derived from the catabolism of muscle lipid and protein. Refeeding starved fishes for 48 hr induced several-fold increases in the rates of in vivo and in vitro incorporation of (14C)glucose into liver and muscle lipid and of (14C)glycine into liver and muscle protein. Incorporation of (14C)glucose into liver glycogen was also increased. However; refeeding did not affect the incorporation of labeled glucose into muscle glycogen, neither in vivo nor in vitro. Administration of pharmacological doses of insulin to normally fed catfishes resulted in marked increases in the in vivo incorporation of 14C from glucose into lipid and protein in both liver and muscle. In contrast, labeled glucose incorporation into muscle glycogen was not affected by insulin and label incorporation into liver glycogen was actually lower than that in noninjected controls.

  16. Digestible energy of crude glycerol for pacu and silver catfish

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    Rafael Ernesto Balen

    2014-01-01

    Full Text Available The increase in global biodiesel production is originating a glycerol surplus, which has no defined destination. An alternative to overcome this problem is its use as energy source in animal feeding. In Brazil, Pacu (Piaractus mesopotamicus is one of the most farmed native fish species, whereas Silver catfish (Rhamdia quelen is suitable for production in subtropical region. Considering little knowledge about crude glycerol utilization in feeds for Neotropical fish species, it was evaluated the apparent digestibility coefficients (ADCs for energy of crude glycerol for P. mesopotamicus and R. quelen. The digestibility and digestible energy content of crude glycerol can be considered excellent even when compared to energy of common ingredients such as maize and wheat, presenting 0.97 and 0.89 of energy ADCs, and 15.2 and 13.95MJ kg-1 of digestible energy for Pacu and Silver catfish, respectively. In conclusion, crude glycerol is an energetic ingredient with good potential in Brazilian native fish diets.

  17. Ammonia excretion at different life stages of silver catfish - 10.4025/actascianimsci.v34i1.11898

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    Bernardo Baldisserotto

    2011-11-01

    Full Text Available This study examined ammonia excretion at different life stages (eggs, larvae and juveniles in silver catfish (Rhamdia quelen and determined the influence of fasting time on ammonia excretion. Eggs and larvae were collected from incubators at different times after fecundation and placed in chambers. Juveniles were separated into two weight classes (2–50 g and 150-320 g and placed in individual chambers after feeding. Water was collected from each chamber to determine ammonia excretion. Ammonia excretion by the eggs was low, but when hatching began approximately 28h after fecundation, excretion increased until 48h after fecundation. In fasting silver catfish juveniles, there was a significant negative correlation between ammonia excretion and weight. Moreover, ammonia excretion decreased significantly after 12 and 48h of fasting (compared to 6h fasting in the smallest and largest specimens, respectively. Consequently, during incubation of silver catfish eggs, water renovation must be increased at hatching time to avoid a build-up in the concentration of ammonia. In addition, as ammonia excretion in this species increases after feeding, feed must be discontinued when ammonia levels in the tanks are high to avoid a further increase of this metabolite and consequent mortality of silver catfish.

  18. Comparative study on effects of dietary with diphenyl diselenide on oxidative stress in carp (Cyprinus carpio) and silver catfish (Rhamdia sp.) exposed to herbicide clomazone.

    Science.gov (United States)

    Menezes, Charlene; Leitemperger, Jossiele; Toni, Cândida; Santi, Adriana; Lópes, Thais; Barbosa, Nilda Berenice Vargas; Neto, João Radünz; Loro, Vania Lucia

    2013-09-01

    The study investigated the capacity of diphenyl diselenide [(PhSe)2] (3.0mg/kg), on reduce the oxidative damage in liver, gills and muscle of carp and silver catfish exposed to clomazone (192h). Silver catfish exposed to clomazone showed increased thiobarbituric acid-reactive substance (TBARS) in liver and muscle and protein carbonyl in liver and gills. Furthermore, clomazone in silver catfish decrease non-protein thiols (NPSH) in liver and gills and glutathione peroxidase and ascorbic acid in liver. (PhSe)2 reversed the effects caused by clomazone in silver catfish, preventing increases in TBARS and protein carbonyl. Moreover, NPSH and ascorbic acid were increased by values near control. The results suggest that (PhSe)2 attenuated the oxidative damage induced by clomazone in silver catfish. The clomazone no caused an apparent situation of oxidative stress in carp, showing that this species is more resistant to this toxicant. Altogether, the containing (PhSe)2 diet helps fish to increase antioxidants defenses.

  19. Crescimento e sobrevivência do catfish de canal (Ictalurus punctatus sp e jundiá (Rhamdia sp no outono-inverno do Rio Grande do Sul

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    Souza Luciano Sena de

    2005-01-01

    Full Text Available O catfish é um peixe de couro, com hábito alimentar onívoro, nativo dos Estados Unidos, tendo despertado interesse dos produtores e indústrias da região sul do Rio Grande do Sul, devido à qualidade da carne, crescimento e facilidade de manejo. O jundiá também é um peixe de couro com hábito alimentar onívoro, apresentando bom desenvolvimento e fácil reprodução, sendo necessário avaliar seu potencial em sistemas de produção intensiva. O objetivo deste trabalho foi comparar o desempenho e sobrevivência do catfish, jundiá cinza e albino. O experimento foi realizado na estação de piscicultura do Chasqueiro, localizada no município de Arroio Grande - RS, no período de abril a agosto de 2001, tendo a duração de 135 dias. Utilizaram-se 120 juvenis de catfish (91,8?25,8g, 120 de jundiá albino (83,7?20,9g e 120 de jundiá cinza (80,4?12,5g, distribuídos em 3 tanques de terra retangulares, com 180m², divididos longitudinalmente em três parcelas, formando um total de 9 divisões de 60m² cada. Os peixes foram colocados em uma densidade de 0,6 peixe/m² da área, e alimentados com ração comercial extrusada (PB 36% na ordem de 3% da biomassa ao dia. Durante o período experimental avaliou-se o ganho de peso, o crescimento e a sobrevivência dos animais. A temperatura da água variou entre 11 e 290C, com média de 18,5degreesC. Os resultados indicaram um ganho de peso no período de 40,4; 74,1 e 109,1g e sobrevivências de 14, 32 e 63% para o catfish, jundiá albino e cinza, respectivamente. Conclui-se que o jundiá cinza apresentou o melhor desempenho produtivo no período analisado.

  20. Reappraisal of Rhamdia branneri Haseman, 1911 and R. voulezi Haseman, 1911 (Siluriformes: Heptapteridae from the rio Iguaçu with notes on their morphometry and karyotype

    Directory of Open Access Journals (Sweden)

    Julio Cesar Garavello

    2016-01-01

    Full Text Available ABSTRACT The species Rhamdia branneri Haseman, 1911 and the subspecies Rhamdia branneri voulezi Haseman, 1911 from rio Iguaçu are currently recognized as synonyms of Rhamdia quelen (Quoy & Gaimard, 1824. However, recent karyotype and ecomorphology studies distinguish R. branneri and R. voulezi as different species. Examination of Rhamdia populations from rio Iguaçu, including type specimens, together with other congeners from rio Tibagi, allowed to properly reexamine the situation of these Haseman's taxa and references given to Rhamdia in the Iguaçu. The species R. branneri and R. voulezi have strong serrae with large basis on both margins of the pectoral-fin spine, uncommon in the remaining species of Rhamdia and different from the fine serrate margins of the pectoral spine of R. quelen ; a regular dorsal profile, slightly curved between supraoccipital and dorsal fin; with dorsal dark-brown or light-gray coloration along body, abdomen pale, without profuse small black spots, common in populations of the upper Paraná species and the type-material of R. quelen . Also the following morphometric characters discriminate these species: Rhamdia voulezi , adipose fin elongate; smaller distance between dorsal and adipose fin; smaller length between adipose fin to base of caudal fin and smaller distance from posterior margin of eye to opercular border. Rhamdia branneri , larger distance from dorsal and adipose fins; deeper caudal peduncle; higher trunk depth in the vertical distance through adipose, between pelvic and anal fins; larger scapular bridge; shorter maxillary barbel; shorter external mental barbel; shorter interorbital distance; shorter length of dorsal fin basis and shorter adipose-fin base length. A PCA between the populations of Rhamdia from Iguaçu and a population from rio Tibagi, upper Paraná basin, discriminates the population from Tibagi on basis of dorsal to adipose fin distance, dorsal-fin spine length, maxillary barbel length

  1. Suplementação de fitase microbiana na dieta de alevinos de jundiá: efeito sobre o desempenho produtivo e as características de carcaça Dietary microbial phytase supplementation of silver catfish fingerlings: effect of performance and carcass characteristics

    Directory of Open Access Journals (Sweden)

    Cleber Bastos Rocha

    2007-12-01

    Full Text Available Este estudo foi realizado para avaliar os efeitos da adição de diferentes níveis de fitase na dieta sobre o desempenho produtivo e as características de carcaça de alevinos de jundiá (Rhamdia quelen. Foram utilizados 208 alevinos com peso vivo inicial médio de 2,92±0,59g, distribuídos em um delineamento inteiramente casualizado com quatro tratamentos (0, 500, 1.000 e 1.500FTU kg-1 e quatro repetições. Na composição das dietas, foram utilizados somente ingredientes de origem vegetal e o alimento foi fornecido duas vezes ao dia. A cada quinzena, foram avaliados os parâmetros produtivos e, ao término do período experimental (45 dias, foram ainda realizadas avaliações nas carcaças. O ganho de peso médio foi significativamente crescente (PThis study evaluated the effects of adding increasing levels of microbial phytase to diets on performance and carcass traits of silver catfish fingerlings (Rhamdia quelen. Two-hundred and eight silver catfish fingerlings (average initial weight: 2.92±0.59g were randomly allotted to 4 treatments (0, 500, 1000 and 1500 phytase units kg-1 of diet with 4 replications, in a completely randomized design. No animal protein was added to the diets. The experiment was conducted for 45 days and fish were fed twice daily. Effects of phytase on performance were measured each 15 days and carcass traits were evaluated at the end of trial. Increasing levels of phytase linearly improved weight gain, from 1.24±0.27g (no phytase to 1.73±0.23g (1500PU kg-1 of diet (P<0.05; and rate of specific growth, from 0.78±0.12% (no phytase to 1.07±0.11% (1500PU kg-1 of diet (P<0.05. However, ether extract of carcass was linearly reduced, from 13.54±0.02% (no phytase to 10.59±0.10% (1500PU kg-1 of diet (P<0.05. Increasing levels of dietary microbial phytase, up to 1500PU kg-1 of diet, improve performance and carcass traits of silver catfish fingerlings.

  2. Utilização de adsorvente em rações contendo aflatoxina para alevinos de jundiá Use of adsorbent in diets containing aflatoxin for silver catfish fingerlings

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    Paulo Rodinei Soares Lopes

    2009-04-01

    Full Text Available Neste trabalho foi avaliado o efeito de um adsorvente (aluminossilicato de cálcio e sódio no desempenho de alevinos de jundiá (Rhamdia quelen alimentados com rações contendo níveis crescentes de aflatoxina. Foram utilizados 360 peixes com peso inicial de 4,2 ± 0,6 g, criados em sistema de recirculação da água termorregulada, durante 90 dias, e alimentados com rações contendo um dos quatro níveis de aflatoxinas (0, 150, 250 e 350 µg/kg, com e sem inclusão de adsorvente (0, 0,3 e 0,6%, com três repetições. O uso de aflatoxina teve efeito negativo sobre o crescimento e o ganho de peso dos alevinos, que reduziram proporcionalmente aos níveis de aflatoxina, mas não ocasionou mortalidade. Os níveis de adsorvente na dieta não diminuíram os efeitos das aflatoxinas no desempenho dos peixes, mas diminuíram a aceitação da ração, em virtude da palatabilidade do produto. Não é recomendável a utilização de aluminossilicato de cálcio e sódio como adsorvente de aflatoxinas em rações para alevinos de jundiá.The effects of aflatoxins and one adsorbent (Sodium and calcium aluminum silicate on silver catfish (Rhamdia quelen fingerlings were evaluated in this study. Three hundred and sixty fish, with initial weight of 4 g, were raised in a thermo-regulated recirculation water system, during 90 days. Twelve treatments with four aflatoxin levels in diet (0, 150, 250 and 350 µg/kg, with and without adsorbents (0, 0.3 and 0.6% were tested in three replications. The results showed that the negative effect of aflatoxins significantly reduced the growth and weight gain of fingerlings, proportionately to the increasing levels of aflatoxin in diet, with mortality occurrence. The adsorbent levels in diet did not diminish the effects of aflatoxin action on fish performance due to the low inclusion and also little acceptance of ration in relation to the product palatability. It is concluded that silver catfish fingerlings fed diets

  3. Ammonia excretion at different life stages of silver catfish=Excreção de amônia em diferentes estágios de vida do jundiá

    Directory of Open Access Journals (Sweden)

    Vania Lúcia Loro

    2012-01-01

    Full Text Available This study examined ammonia excretion at different life stages (eggs, larvae and juveniles in silver catfish (Rhamdia quelen and determined the influence of fasting time on ammonia excretion. Eggs and larvae were collected from incubators at different times after fecundation and placed in chambers. Juveniles were separated into two weight classes (2-50 g and 150-320 g and placed in individual chambers after feeding. Water was collected from each chamber to determine ammonia excretion. Ammonia excretion by the eggs was low, but when hatching began approximately 28h after fecundation, excretion increased until 48h after fecundation. In fasting silver catfish juveniles, there was a significant negative correlation between ammonia excretion and weight. Moreover, ammonia excretion decreased significantly after 12 and 48h of fasting (compared to 6h fasting in the smallest and largest specimens, respectively. Consequently, during incubation of silver catfish eggs, water renovation must be increased at hatching time to avoid a build-up in the concentration of ammonia. In addition, as ammonia excretion in this species increases after feeding, feed must be discontinued when ammonia levels in the tanks are high to avoid a further increase of this metabolite and consequent mortality of silver catfish.Verificou-se a excreção de amônia em diferentes estágios de vida (ovos, larvas e juvenis e determinou-se a influência do tempo de jejum na excreção de amônia no jundiá, Rhamdia quelen. Ovos e larvas foram coletados das incubadoras em diferentes tempos após a fecundação e colocados em recipientes. Juvenis foram separados em duas classes de peso (2-50 e 150-320 g e após a alimentação foram colocados em recipientes individuais. As águas foram coletadas de cada recipiente para determinação da excreção de amônia. A excreção de amônia pelos ovos foi baixa, mas iniciada a eclosão – 28h após a fecundação, ela aumentou até as 48h. Nos

  4. Anesthesia of silver catfish with eugenol: time of induction, cortisol response and sensory analysis of fillet Anestesia de jundiás com eugenol: tempo de indução, resposta ao cortisol e análise sensorial do filé

    Directory of Open Access Journals (Sweden)

    Mauro Alves da Cunha

    2010-10-01

    Full Text Available The aim of this study was to identify the time of anesthetic induction and recovery of silver catfish (Rhamdia quelen exposed to eugenol. It was also determined the efficacy of the anesthetic as a stress reducing agent and performed a sensory analysis of the fillets from fish exposed to this substance. The silver catfish were exposed to air for 1min to carry out biometry, and blood was collected at 0, 1 and 4 hours later. Eugenol can be used in the range of 20-50mg L-1 for anesthetic induction in silver catfish, and recovery time from anesthesia was not affected by eugenol concentration. The control group showed significantly higher cortisol levels 4 hours after biometry than at time zero. Fish anesthetized with eugenol (50mg L-1 presented significantly lower plasma cortisol levels than control fish at the same time. These data indicate that eugenol inhibits the rise of cortisol in the blood. The sensory analysis test demonstrated that eugenol modifies the flavor of the fillet and therefore is contra-indicated for anesthetization of silver catfish that are intended for human consumption.O objetivo deste estudo foi identificar o tempo de indução e recuperação anestésica de jundiás (Rhamdia quelen expostos ao eugenol, bem como a eficácia desse anestésico na inibição do estresse e realizar análise sensorial dos filés dos peixes expostos a essa substância. Os jundiás foram expostos ao ar por um minuto para realização da biometria, e o sangue foi coletado zero, uma e quatro horas depois. O eugenol pode ser usado na faixa de 20-50mg L-1 para a indução da anestesia em jundiás, e o tempo de recuperação da anestesia não foi afetado pela concentração do eugenol. O grupo de controle mostrou níveis significativamente mais elevados do cortisol quatro horas após a biometria que no tempo zero. Os peixes anestesiados com eugenol (50mg L-1 apresentaram níveis significativamente mais baixos do cortisol plasmático do que peixes do grupo

  5. Neotropical Monogenoidea. 57. Revision and phylogenetic position of Scleroductus Jara & Cone, 1989 (Gyrodactylidae), with descriptions of new species from the Guatemalan chulin Rhamdia guatemalensis (Günther) (Siluriformes: Heptapteridae) in Mexico and the barred sorubim Pseudoplatystoma fasciatum (Linnaeus) (Siluriformes: Pimelodidae) in Brazil.

    Science.gov (United States)

    Kritsky, Delane C; Boeger, Walter A; Mendoza-Franco, Edgar F; Vianna, Rogério T

    2013-01-01

    The diagnosis of Scleroductus Jara & Cone, 1989 (Gyrodactylidae) is amended to include viviparous species having a large spine associated with the ejaculatory duct within the male copulatory organ, two band-like projections originating from the posterior margin of the superficial bar and hooks evenly distributed along the haptoral margins. Two new species of Scleroductus are described from the external surfaces of siluriform fishes of Mexico and Brazil: Scleroductus lyrocleithrum n. sp. from the Guatemalan chulin Rhamdia guatemalensis (Günther) (Heptapteridae) in a cenote on the Yucatan Peninsula, Mexico; and Scleroductus angularis n. sp. from the barred sorubim Pseudoplatystoma fasciatum (Linnaeus) (Pimelodidae) in the Rio Tocantins, Tocantins, Brazil. Scleroductus sp. is recorded from the jundiá Rhamdia quelen (Quoy & Gaimard) (Heptapteridae) in the Iguaçu drainage of southern Brazil. Phylogenetic analyses of 18S rDNA suggest that Scleroductus (represented in the analyses by Scleroductus sp. from R. quelen), Ieredactylus Schelkle, Paladini, Shinn, King, Johnson, van Oosterhout, Mohammed & Cable, 2011 (represented by its type-species) and Gyrodactyloides Bychowsky, 1947 (represented by G. bychowskii Al'bova, 1948) comprise the sister taxon to the remaining viviparous gyrodactylids, with Scleroductus serving as the sister group to Ieredactylus + Gyrodactyloides. The clade containing the three taxa is supported by two putative synapomorphic features: two band-like projections (R1 sclerites) originating from the posterior margin of the superficial bar and a large spine associated with the ejaculatory duct within the male copulatory organ. PMID:23263939

  6. Rendimento corporal e composição química de jundiás alimentados com diferentes níveis de proteína e energia na dieta, criados em tanques-rede Catfish (jundia body yield and chemical composition fed different protein and energy level in the diet, reared in net-tanks

    Directory of Open Access Journals (Sweden)

    Adilson Reidel

    2010-02-01

    Full Text Available Objetivou-se analisar o rendimento corporal e a composição química de jundiás, Rhamdia quelen, alimentados com rações contendo diferentes níveis de proteína e energia e criados em sistema de tanques-rede. Utilizou-se a densidade inicial de 70 peixes/m³, em 18 tanques-rede (5,0 m³, durante 324 dias. O delineamento experimental foi inteiramente casualizado com dois fatores - níveis de proteína bruta (PB, 25, 30 ou 35%; e níveis de energia digestível (ED: 3.250 e 3.500 kcal - e três repetições. A dieta foi fornecida à vontade, mas mensurada diariamente. No início, um lote de peixes foi sacrificado para determinação dos parâmetros corporais e químicos. A cada 28 dias, dois peixes de cada unidade experimental foram abatidos para determinação dos comprimentos total e padrão, do peso total e dos pesos de fígado, gônadas, cabeça, tronco limpo e gordura visceral, respectivamente. Posteriormente, foram realizados cálculos dos índices hepatossomático e gonadossomático, da porcentagem de cabeça e do rendimento de carcaça e do rendimento de tronco limpo. A partir do mês de junho de 2006, também foram registrados o rendimento de filé e o sexo dos indivíduos. Para determinação da composição da carne do jundiá, foram realizadas as análises químicas de umidade, proteína bruta, lipídios e matéria mineral. O fornecimento da ração com 30% de PB resultou em maior rendimento de tronco limpo. A composição do músculo não foi influenciada pelas dietas testadas. Os melhores resultados foram obtidos com a utilização de dietas contendo 30% de PB e 3.250 kcal de energia digestível/kg.The objective of this study was to analyze the body yield and the chemical composition of catfish (jundia, Rhamdia quelen, reared in net-tanks and fed with diets containing different levels of protein and energy. An initial density of 70 fish/m³, in 18 net-tanks (5.0 m³ was used, for 324 days. A randomized complete design was used

  7. Pseudocapillaria yucatanensis sp. n. (Nematoda: Capillariidae) from the bagre Rhamdia guatemalensis (Pisces) from cenotes in Yucatan, Mexico.

    Science.gov (United States)

    Moravec, F; Scholz, T; Vivas Rodríguez, C

    1995-01-01

    A new nematode species, Pseudocapillaria yucatanensis sp. n., is described from the intestine of the freshwater pimelodid catfish Rhamdia guatemalensis (Günther) from cenotes (= sinkholes) in Yucatan, Mexico. It differs from other three related species parasitizing freshwater fishes mainly in possessing the spicule with a simple rim of its proximal end and a non-expanded distal end, in the length of the spicule (0.218-0.295 mm), and the size (0.050-0.060 x 0.025-0.030 mm), shape and structure of eggs, and also in the host types and geographical distribution. Pseudocapillaria yucatanensis is the first known autochtonous species of Pseudocapillaria parasitizing freshwater fishes in Mexico. PMID:9599428

  8. Desenvolvimento gonadal do jundiá, Rhamdia quelen (Teleostei, Siluriformes, em viveiros de terra, na região sul do Brasil = Gonadal development of jundiá, Rhamdia quelen (Teleostei, Siluriformes, in earthen ponds in southern Brazil

    Directory of Open Access Journals (Sweden)

    Luciana Ghiraldelli

    2007-10-01

    Full Text Available O presente estudo caracterizou o desenvolvimento gonadal de jundiá até a maturação sexual, quando cultivados em viveiros de terra, visando subsidiar o desenvolvimento de tecnologia de cultivo para esta espécie na região sul do Brasil. Alevinos de jundiá (peso médio 8 ± 3,73 g foram estocados em três viveiros, na densidade de 0,6indivíduos m-2. Catorze indivíduos foram amostrados mensalmente, de setembro de 2001 a outubro de 2002. Os estádios de maturação gonadal foram caracterizados macroscopicamente, e fragmentos de ovários e testículos de alguns exemplares foram fixados em formalina 4% para análise histológica. Foram analisados 118 exemplares: 60 machos e 58 fêmeas. Os machos apresentaram atividade reprodutiva precoce, quandocomparados às fêmeas. Testículos e ovários apresentaram morfologia similar a de outras espécies de Siluriformes. De acordo com a análise microscópica, as gônadas foram classificadas em quatro estádios de desenvolvimento: imaturo, em maturação inicial, em maturação final e maduro. A relação gonadossomática variou de 0,29 a 9,16 para os machose de 0,11 a 13,03 para as fêmeas. Indivíduos maduros foram observados nos meses de verão (dezembro/2001 e janeiro/2002, outono (abril e maio/2002 e primavera (setembro e outubro/2002, acompanhando o aumento de temperatura.The study characterized the gonadal development of jundiá from approximately 8 g until gonadal maturation, to provide further knowledge for farming jundiá in Southern Brazil. Jundiá fingerlings(average weight 8.00 ± 3.73 g were stocked in three ponds at 0.6 fish m-2. Fourteen fish were sampled each month from September 2001 to October 2002. Gonadal maturation stages were characterized macroscopically, and samples of ovaries and testicles were fixedin 4-%-buffered formalin for histological examination. One hundred and eighteen fish were analyzed: 60 males e 58 females. Jundiá males matured earlier than females. Testicles and ovaries presented similar morphology to other Siluriformes species. Gonads were classified in four development phases of the ovarian follicle, according to histology: immature, initial maturation, final maturation and mature. The gonadosomatic index varied from 0.29 to 9.16 for males and from 0.11 to 13.03 for females. Mature individuals were observed during summer (December/2001 and January/2002, fall (April and May/2002 and spring (September and October/2002, following the increase in water temperature.

  9. Protein sources and digestive enzyme activities in jundiá (Rhamdia quelen Fontes protéicas e atividade de enzimas digestivas em jundiás (Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    Rafael Lazzari

    2010-01-01

    Full Text Available Digestive enzymes activity influence feed utilization by fish, and its understanding is important to optimize diet formulation. This study reports the digestive enzyme activities of jundiá juveniles fed diets with protein sources. Fish were fed six experimental diets for 90 days: MBY (meat and bone meal + sugar cane yeast, SY (soybean meal + sugar cane yeast, S (soybean meal, MBS (meat and bone meal + soybean meal, FY (fish meal + sugar cane yeast and FS (fish meal + soybean meal, and then sampled every 30 days and assayed in two intestine sections for digestive enzymes - trypsin, chymotrypsin and amylase - activities; gastric protease was assayed in the stomach. Digestive and hepatosomatic index, intestinal quotient, digestive tract length and weight gain were also measured. Trypsin and chymotrypsin activities were higher (p As enzimas digestivas influenciam a utilização dos alimentos em peixes, e seu conhecimento é importante para otimizar a formulação de dietas. Este trabalho descreve a atividade de enzimas digestivas em juvenis de jundiá alimentados com fontes protéicas. Os peixes foram alimentados com seis dietas (90 dias: MBY (farinha de carne e ossos + levedura de cana, SY (farelo de soja + levedura de cana, S (farelo de soja, MBS (farinha de carne e ossos + farelo de soja, FY (farinha de peixe + levedura de cana e FS (farinha de peixe + farelo de soja. A cada 30 dias, foram analisadas as enzimas digestivas (tripsina, quimiotripsina e amilase no intestino. No estômago, foi mensurada a protease ácida. Foram estimados os índices digestivo e hepato-somático, quociente intestinal, comprimento do trato digestório e ganho em peso. As atividades de tripsina e quimiotripsina foram maiores (p < 0,0001 nos peixes alimentados com as dietas com fontes de origem animal (MBY, MBS, FY e FS. As proteases alcalinas foram afetadas negativamente pelo farelo de soja nas dietas. A atividade de amilase apresentou grandes variações. Os peixes alimentados com as dietas MBY e MBS apresentaram maior atividade de protease ácida (p < 0,0001. Os ganhos em peso foram maiores nos peixes alimentados com as dietas MBS e FS (p < 0,05. Os maiores valores (p < 0,05 de comprimento do trato digestório foram verificados nos peixes alimentados com as dietas MBS e FY. A composição da dieta influencia na atividade de enzimas digestivas do jundiá.

  10. Feeding strategy of the jundiá Rhamdia quelen (Siluriformes, Heptapteridae in costal lagoons of southern Brazil = Estratégia alimentar do jundiá Rhamdia quelen (Siluriformes, Heptapteridae nas lagoas costeiras do sul do Brasil

    Directory of Open Access Journals (Sweden)

    Marlise de Azevedo Bemvenuti

    2009-01-01

    Full Text Available Feeding strategy and mouth morphology of ‘jundiá’ in costal lagoons of south of Rio Grande do Sul were studied. Gut contend of 189 individuals were collected from 2002 to 2004 and analysed according Amundsen. Fishes and crustaceans were the feeding items morefrequent in the diet. Mollusk, insects and plants had low FO%. Season variation in diet showed lower variety of feeding items during summer and higher in winter. The diet vary according to site were irrigation channels showed low FO% for all items. The ‘jundiá’ showed generalistfeeding strategy, generally preying on fish and crustaceous. On the description of its feeding apparatus the importance on the capture prey was observed.Neste trabalho, foi analisada a estratégia alimentar e a morfologia da boca do jundiá que ocorre nas lagoas costeiras do sul do RioGrande do Sul. Foram coletados 189 indivíduos entre os anos de 2002 e 2004 e o conteúdo estomacal foi analisado de acordo com Amundsen. Os itens alimentares mais frequentes na dieta foram peixes e crustáceos. Outros itens como moluscos, insetos e plantas apresentaram baixaFO%. Na análise por estações do ano, observou-se menor diversidade de itens alimentares no verão e maior no inverno. A dieta variou de acordo com o local de coleta e nos canais de irrigação todos os itens apresentaram baixa FO%. O jundiá apresentou estratégia alimentar generalista, com tendência a predar peixes e crustáceos. Na descrição morfológica, foi observada a importância das estruturas bucais na captura do alimento.

  11. Transport of jundiá Rhamdia quelen juveniles at different loading densities: water quality and blood parameters

    Directory of Open Access Journals (Sweden)

    Paulo César Falanghe Carneiro

    2009-06-01

    Full Text Available Fish transportation is a common practice on fish farms and is considered to be a stressor that could negatively affect fish health. The objective of this study was to evaluate several physiological responses of stress in jundiá caused by transport at different loading densities. Jundiá juveniles were placed in plastic bags on a mechanical transport simulator for four hours at four different loading densities (75, 150, 250 and 350 g L-1 and then transferred to sixteen 80-L plastic boxes for 96 hours after transport. Water samples were collected before and after transport to measure dissolved oxygen, temperature, pH and ammonia levels. Blood samples were taken at departure and arrival, as well as at 24 and 96 hours after transport to monitor cortisol, glucose, ammonia, chloride and hematocrit levels. Water ammonia levels were found to increase gradually as loading densities increased. Plasma ammonia was higher after transport in fish from all treatments. Compared to initial values, substantial increases in plasma cortisol and ammonia levels were detected mainly in those fish submitted to the highest loading density. Blood glucose appeared to be positively influenced by the increase of transport densities. No statistical differences were observed in any of the other blood parameters. The costs in fish culture, as in other animal production systems, must be minimized and fish producers depend on optimal techniques to ensure better profit. Therefore, based on fish survival and the physiological indicators determined in the present study, especially during recovery, the best density at which to transport jundiá in plastic bags for four hours is about 350 g/L.O transporte de peixes é uma prática comum em piscicultura e considerado como um agente estressor que causa efeitos negativos na saúde do peixe. O objetivo deste estudo foi avaliar algumas respostas fisiológicas de estresse no jundiá causadas pelo transporte em densidades diferentes. Juvenis de jundiá foram transportados em sacos plásticos num simulador de transporte por quatro horas em diferentes densidades (75, 150, 250 e 350 g L-1 e transferidos para 16 caixas plásticas de 80 L por 96 horas após o transporte. Amostras de água foram coletadas antes e após o transporte para determinações de oxigênio dissolvido, temperatura, pH e amônia. Além dos momentos da saída e da chegada, amostras de sangue foram retiradas 24 e 96 horas após o transporte para monitorar os níveis de cortisol, glicose, amônia, cloreto e hematócrito. A amônia na água aumentou gradualmente acompanhando o aumento das densidades. A amônia plasmática estava elevada após o transporte nos peixes de todos os tratamentos. Comparando com os valores iniciais, aumentos substanciais nos níveis plasmáticos de cortisol e amônia foram registrados principalmente nos peixes submetidos à densidade de transporte mais elevada. Os níveis glicêmicos parecem ter sido influenciados pelo aumento nas densidades de transporte. Não foram registradas diferenças significativas nos demais parâmetros sanguíneos. O custo da criação de peixes, da mesma forma que de outros animais, deve ser minimizado e os produtores dependem de técnicas que permitam lucros maiores. Portanto, com base nos indicadores fisiológicos e na taxa de sobrevivência obtidos no presente estudo, especialmente considerando o período de recuperação, sugere-se que a melhor densidade para o transporte do jundiá em sacos plásticos por quatro horas seja de aproximadamente 350 g/L.

  12. Catfish culture in Southeast Asia

    OpenAIRE

    Adan, R. I. Y.

    2000-01-01

    Catfish rank fifth in the world in terms of fresh and brackishwater fish culture. In Asia and the Pacific, the Clariidae family dominates production, representing nearly 80% of the total catfish production. Among the most cultured species are Clarias batrachus, C. macrocephalus, C. gariepinus. The domestic market generally absorbs catfish produce in Asia, although high-producing countries like Thailand and Vietnam engage in export. There are two basic markets for catfish: live fish and proces...

  13. Growing catfish in the Philippines

    OpenAIRE

    Surtida, Marilyn B.; Buendia, Romeo Y.

    2000-01-01

    The catfish industry in the Philippines is budding and projected to expand in the coming years. This is evident from conversations with active catfish farmers who all hope to be able to expand production, whether backyard or commercial because their present production can hardly supply the demands of buyers. NIFTDC, a fisheries technology and development center in Dagupan City, Philippines, however, says that unless the government has a catfish program, expansion of the industry would be slow...

  14. Catfish - King of the sea

    OpenAIRE

    Godø, Olav Rune; Huse, Irene; Michalsen, Kathrine

    1995-01-01

    During an acoustic tagging experiment on cod in the Barents Sea in March 1995 a hierarchy between different fish species was revealed, and the catfish (Anarhichas sp.) was observed to be dominant in relation to cod and haddock. When catfish are present at the fishing grounds, the dominant feeding behaviour of this species might reduce efficiency on other species. If longline catch data are used for stock assessment purposes, the catfish population might therefore be overestimat...

  15. Evaluation of immune responses against the parasite Ichthyophthirius multifiliis between channel catfish and hybrid catfish

    Science.gov (United States)

    Channel catfish is a dominant aquaculture species in the USA and has been cultured for several decades. Recently, an increasing number of producers are showing an interest in the culture of hybrid catfish (channel catfish × blue catfish) instead of channel catfish due to its performance traits. The ...

  16. Carbohydrate metabolism in catfish

    International Nuclear Information System (INIS)

    Radiolabeled (U- 14C)-glucose was incorporated in diets and forced-fed to channel catfish and was observed for a 24 hour period. About 95% of fed labeled (U-14C)-glucose was absorbed by catfish, showing a high digestibility of glucose. The amounts of 14C excreted over 24 h as carbon dioxide were 49% and amounts excreted in urine were 3.5%. The amount retained as protein, fat glycogen and other organic compounds were 8.2, 1.2, 6.5 and 32.1 % respectively, for the 24 hour period. The blood concentration of 14 C reached a maximum 2.5 hour after feeding (U-14C)-glucose, then gradually decreased. Based on tissue concentrations of 14C, glycogen was an immediate storage site for absorbed glucose, but 14C- glycogen in liver decreased rapidly. Glucose was quickly and heavily converted into triglyceride, indicating that fat is an important intermediate in the metabolism of glucose in channel catfish. 14C-fat in the serum and liver were transferred to the adipose tissue in the muscle and mesentery about 10 hours after feeding. (Author)

  17. Catfish production using intensive aeration

    Science.gov (United States)

    For the last 3 years, researchers at UAPB and NWAC have been monitoring and verifying production yields in intensively aerated catfish ponds with aeration rates greater than 6 hp/acre. We now have three years of data on commercial catfish production in intensively aerated ponds. With stocking densi...

  18. The catfish genome database cBARBEL: an informatic platform for genome biology of ictalurid catfish

    OpenAIRE

    Lu, Jianguo; Peatman, Eric; Yang, Qing; Wang, Shaolin; Hu, Zhiliang; Reecy, James; Kucuktas, Huseyin; Liu, Zhanjiang

    2010-01-01

    The catfish genome database, cBARBEL (abbreviated from catfish Breeder And Researcher Bioinformatics Entry Location) is an online open-access database for genome biology of ictalurid catfish (Ictalurus spp.). It serves as a comprehensive, integrative platform for all aspects of catfish genetics, genomics and related data resources. cBARBEL provides BLAST-based, fuzzy and specific search functions, visualization of catfish linkage, physical and integrated maps, a catfish EST contig viewer with...

  19. Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor

    NARCIS (Netherlands)

    Vischer, HF; Granneman, JCM; Linskens, MHK; Schulz, RW; Bogerd, J

    2003-01-01

    LH and FSH are heterodimeric glycoprotein hormones, composed of a common alpha-subunit non-covalently associated with a hormone-specific beta-subunit. Repeated efforts to isolate catfish FSH (cfFSH) have not been successful and only catfish LH (cfLH) has been purified from catfish pituitaries. Recen

  20. Comparative Oxygen Tolerance of Blue Catfish Ictalurus furcatus and Channel Catfish I. punctatus

    Science.gov (United States)

    Blue catfish (Ictalurus furcatus) have several advantages over channel catfish (I. punctatus) as commercial culture fish. They are more resistant to ESC, PGD, and CCVD, major diseases of channel catfish. They are very easy to seine, with a near-total harvest possible with one seine haul, and may ex...

  1. Catfish Preservation using Porphyra Yezoensis Composites Preservatives

    OpenAIRE

    Zhi-Gang Qian; Long-Fa Jiang; Li-Qiang Rui

    2013-01-01

    This study aims to preserve fresh catfish meat by using Porphyra Yezoensis extract, chitosan and lactic acid Nisin. The composite preservative obtained by sensory evaluation can effectively maintain the color, odor and texture of fresh catfish meat, as well as inhibit bacterial growth. Results show that treatment using a preservative solution (Porphyra Yezoensis extract 10%, Nisin 0.2% and chitosan 15%) extended the shelf life of the fresh catfish meat from 12 h to 24 h when stored at room te...

  2. Catfish Preservation using Porphyra Yezoensis Composites Preservatives

    Directory of Open Access Journals (Sweden)

    Zhi-Gang Qian

    2013-09-01

    Full Text Available This study aims to preserve fresh catfish meat by using Porphyra Yezoensis extract, chitosan and lactic acid Nisin. The composite preservative obtained by sensory evaluation can effectively maintain the color, odor and texture of fresh catfish meat, as well as inhibit bacterial growth. Results show that treatment using a preservative solution (Porphyra Yezoensis extract 10%, Nisin 0.2% and chitosan 15% extended the shelf life of the fresh catfish meat from 12 h to 24 h when stored at room temperature and from 6 d to 9 d when stored at 4°C. These results provide a practical method of preserving fresh catfish meat.

  3. Water quality in hybrid catfish ponds after partial fish harvest

    Science.gov (United States)

    Intensification of United States catfish aquaculture involves hybrid catfish ('channel catfish Ictalurus punctatus x ' blue catfish I. furcatus) grown in ponds with abundant aeration and high feeding rates. High feeding rates cause water quality deterioration because most of the nitrogen, phosphorus...

  4. Pyridoxine requirements of channel catfish.

    Science.gov (United States)

    Andrews, J W; Murai, T

    1979-04-01

    In 20 and 12 week feeding trials, channel catfish fingerlings were fed purified diets containing five levels (0, 5, 10, 20, and 30 mg/kg) and six levels (0, 1, 2, 5, 10, and 20 mg/kg) of supplemental pyridoxine hydrochloride. Fish fed unsupplemented diets (pyridoxine content of 1.2 mg/kg) were characterized by anoxeria, nervous disorders, tetany, greenish-blue body coloration, and eventual mortality. Anemia, which has been reported in pyridoxine deficient salmonids, was not observed in pyridoxine deficient catfish. However, a microcytic, normochromic anemia was observed in groups fed high dietary levels of pyridoxine (20 mg/kg or greater). The dietary pyridoxine level required for maximal growth was approximately 3 mg/kg of diet. All other deficiency signs were prevented by 2.2 mg/kg of diet. PMID:430257

  5. Pathogenicity of Streptococcus ictaluri to Channel Catfish

    Science.gov (United States)

    The infectivity of a Streptococcus ictaluri isolate for fry (0.5 g), fingerling (15 g), and juvenile (55 g) channel catfish (Ictalurus punctatus) was determined by bath immersion and injection infectivity experiments. Channel catfish exposed by immersion were exposed to baths containing 1012, 1011,...

  6. Stunning of farmed Afircan catfish( Clarias gariepinus)

    NARCIS (Netherlands)

    Lambooij, S.; Kloosterboer, R.J.; Pieterse, C.; Gerritzen, M.A.; Vis, van de J.W.

    2003-01-01

    The objective of this study was to assess whether high-pressure injection of air into the brain of African catfish (Clarias gariepinus) could render the animal unconscious and insensible immediately and permanently. In the study, 48 African catfish with a live weight of 900-1900 g were restrained an

  7. THE EFFECTIVENESS OF STATE LOGOS FOR FARM-RAISED CATFISH

    OpenAIRE

    Schupp, Alvin R.; Dellenbarger, Lynn E.

    1993-01-01

    Product differentiation can consist of identifying the corporate firm that produces the product (such as Kraft) or the producer cooperative that produces the product (such as Ocean Spray). The Catfish Institute (funded by producers, feed mills and processors) was created to promote the generic sales of farm-raised catfish. Also, a number of Mississippi catfish processors are differentiating their product by promoting it as Mississippi Farm-Raised Catfish. Louisiana farm-raised catfish are hig...

  8. Evaluation of stomach tubes and gastric lavage for sampling diets from blue catfish and flathead catfish

    Science.gov (United States)

    Waters, D.S.; Kwak, T.J.; Arnott, J.B.; Pine, William E., III

    2004-01-01

    We compared the ability to extract all stomach contents by using stomach tubes or gastric lavage to sample diets from blue catfish Ictalurus furcatus and flathead catfish Pylodictus olivarus. Pulsed gastric lavage (PGL) removed a significantly greater proportion of stomach content mass (95.6%) from blue catfish than did stomach tubes (14.6%). Percent mass of flathead catfish contents removed with PGL (96.0%) was not significantly different from that removed with stomach tubes (86.9%). Based on the greater effectiveness of PGL for blue catfish, combined with a shorter mean time required per sample (69 versus 118 s) and the better preservation of extracted diet material, we recommend using PGL as a nonlethal technique to collect diet samples from large catfishes.

  9. The World Demand for Catfish Pangasius

    DEFF Research Database (Denmark)

    Nguyen, Thong Tien; Roth, Eva; Nielsen, Max;

    2014-01-01

    in filleted form. The demand system includes seven equations representing for most important markets that are ASEAN & EAST ASIA, NORTH AMERICA, OCEANIA, RUSIAN & EASTERN EU, SOUTH & CENTRAL AMERICA, WESTERN EU, and ROW (rest of the world) markets. The monthly data are updating from January 2007 to March 2014....... Direct elasticity including own- and cross- price elasticity and income elasticity are calculated to show how consumers from different markets of the world prefer for the Pangasius catfish. We found that catfish products have big room of market demand, indicated by absolute values of own price......In this paper we present a world demand system for Pangasius catfish products. We use solely exporting data from Vietnam for estimating a non-linear Almost Ideal Demand System because Vietnam accounts for more than 90% catfish export value of the world and the products exported are mostly...

  10. Development of 'Serunding' from African catfish

    DEFF Research Database (Denmark)

    Juhari, Nurul Hanisah Binti; MK, Zainol; MM, Masduki

    This research was conducted to develop serunding from African catfish (Clarias gariepinus). Proximate analysis, physical analysis, ascorbic acid analysis, mineral analysis and sensory evaluation were carried out to determine the nutrient compositions and consumer acceptance towards the products. ...

  11. Development of 'Serunding' from African catfish

    OpenAIRE

    Juhari, Nurul Hanisah Binti; MK, Zainol; MM, Masduki

    2009-01-01

    This research was conducted to develop serunding from African catfish (Clarias gariepinus). Proximate analysis, physical analysis, ascorbic acid analysis, mineral analysis and sensory evaluation were carried out to determine the nutrient compositions and consumer acceptance towards the products. samples of serunding were prepared with 3 different sizes (30-40cm, 40-50cm, 50-60cm) of African catfish and control was prepared using round scad fish ('ikan selayang') with same amount of fish flesh...

  12. Catfish stings: A report of two cases

    OpenAIRE

    Gholamali Dorooshi

    2012-01-01

    Venomous catfish stings are a common environment hazard worldwide. Although these stings are often innocuous, significant morbidity may result from stings, including severe pain, retained foreign bodies, infection, respiratory compromise, arterial hypotension, and cardiac dysrhythmias. Treatment included hot water immersion, analgesia, wound exploration, and prophylactic antibiotics. In this article, two cases of stings by catfish referred to the poison center of Noor Hospital, Isfahan Univer...

  13. Catfish stings: A report of two cases

    Directory of Open Access Journals (Sweden)

    Gholamali Dorooshi

    2012-01-01

    Full Text Available Venomous catfish stings are a common environment hazard worldwide. Although these stings are often innocuous, significant morbidity may result from stings, including severe pain, retained foreign bodies, infection, respiratory compromise, arterial hypotension, and cardiac dysrhythmias. Treatment included hot water immersion, analgesia, wound exploration, and prophylactic antibiotics. In this article, two cases of stings by catfish referred to the poison center of Noor Hospital, Isfahan University of Medical Sciences and their treatments have been reported.

  14. DYNAMIC FACTORS INFLUENCING U.S. AND REGIONAL CATFISH DEMAND

    OpenAIRE

    Houston, Jack E.; Ermita, Isabel

    1992-01-01

    Response to changes in factors influencing consumption of catfish and competing commodities differ between national, South Atlantic, and Southwest Central markets. A modified state adjustment model for catfish, beef, chicken, and other fish explicitly included age distribution, residence, occupation, education, and race/ethnic variables associated with habit formation. Nationally, per capita expenditures on catfish respond to present and past relative prices, and catfish, chicken, and other f...

  15. Comparative production of channel catfish and channel x blue hybrid catfish subjected to two minimum dissolved oxygen concentrations

    Science.gov (United States)

    The effect of daily minimum dissolved oxygen concentration on growth and yield (kg/ha) of the channel catfish (Ictalurus punctatus) and the channel x blue hybrid catfish (I. punctatus female x I. furcatus male), which shared the Jubilee strain of channel catfish as the maternal parent, was evaluated...

  16. The Organoleptic and Smoked Catfish Histology from Pre-cooking

    OpenAIRE

    Venny Yuliastri; Ruddy Suwandi; Uju

    2015-01-01

    Catfish is one of the main commodities in fresh water aquaculture. Indonesia catfish production increased 37,49% in 2010 until 2014. Protein content of catfish is 17.7-26.7% and fat about 0.95 until 11.5%. The objective of this study was to determine the best organoleptic and to study the changes of tissue structure of catfish caused by process precooking and smoking process. Precooked Catfish with variation 5, 10 and 15 minute; temperature of 100oC, and smoked for 7 hours with...

  17. Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor

    OpenAIRE

    Vischer, HF; Granneman, JCM; Linskens, MHK; Schulz, RW; Bogerd, J

    2003-01-01

    LH and FSH are heterodimeric glycoprotein hormones, composed of a common alpha-subunit non-covalently associated with a hormone-specific beta-subunit. Repeated efforts to isolate catfish FSH (cfFSH) have not been successful and only catfish LH (cfLH) has been purified from catfish pituitaries. Recently, however, we succeeded in cloning the cDNA encoding the putative cfFSHbeta; the cDNAs for the alpha- and beta-subunit of cfLH have been cloned before. Here we report the expression of biologica...

  18. Feeding strategy of the jundiá Rhamdia quelen (Siluriformes, Heptapteridae in costal lagoons of southern Brazil - DOI: 10.4025/actascibiolsci.v31i1.335

    Directory of Open Access Journals (Sweden)

    Alex Moresco

    2009-04-01

    Full Text Available Feeding strategy and mouth morphology of ‘jundiá’ in costal lagoons of south of Rio Grande do Sul were studied. Gut contend of 189 individuals were collected from 2002 to 2004 and analysed according Amundsen. Fishes and crustaceans were the feeding items more frequent in the diet. Mollusk, insects and plants had low FO%. Season variation in diet showed lower variety of feeding items during summer and higher in winter. The diet vary according to site were irrigation channels showed low FO% for all items. The ‘jundiá’ showed generalist feeding strategy, generally preying on fish and crustaceous. On the description of its feeding apparatus the importance on the capture prey was observed.

  19. CONCURRENT EXPERIMENTAL Streptococcus SPP. INFECTIONS AND NATURAL PARASITISM IN CHANNEL CATFISH Ictalurus punctatus

    Science.gov (United States)

    Streptococcus iniae and S. agalactiae are usually not considered pathogens of channel catfish, Ictalurus punctatus, though concurrent infections may decrease catfish survival when infected with streptococcal organisms. Non-parasitized or naturally-parasitized channel catfish fry were challenged wit...

  20. Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs

    OpenAIRE

    Dunham Rex; Muir William; Liu Lei; Turan Cemal; Simmons Micah; Serapion Jerry; Somridhivej Benjaporn; Nandi Samiran; Kucuktas Huseyin; Xu Peng; Baoprasertkul Puttharat; He Chongbo; Feng Jinian; Wang Shaolin; Peatman Eric

    2007-01-01

    Abstract Background EST sequencing is one of the most efficient means for gene discovery and molecular marker development, and can be additionally utilized in both comparative genome analysis and evaluation of gene duplications. While much progress has been made in catfish genomics, large-scale EST resources have been lacking. The objectives of this project were to construct primary cDNA libraries, to conduct initial EST sequencing to generate catfish EST resources, and to obtain baseline inf...

  1. Recent improvements in channel catfish reproduction

    Science.gov (United States)

    Control of reproduction in fish is a primary requisite for reliable, predictable and quality seed stock for aquaculture production or a stock enhancement program. Channel catfish is the leading aquaculture species in USA, accounting for 335 million pounds with a farm gate value of over $ 400 millio...

  2. Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus and blue catfish (Ictalurus furcatus.

    Directory of Open Access Journals (Sweden)

    Fei Chen

    Full Text Available BACKGROUND: Genome annotation projects, gene functional studies, and phylogenetic analyses for a given organism all greatly benefit from access to a validated full-length cDNA resource. While increasingly common in model species, full-length cDNA resources in aquaculture species are scarce. METHODOLOGY AND PRINCIPAL FINDINGS: Through in silico analysis of catfish (Ictalurus spp. ESTs, a total of 10,037 channel catfish and 7,382 blue catfish cDNA clones were identified as potentially encoding full-length cDNAs. Of this set, a total of 1,169 channel catfish and 933 blue catfish full-length cDNA clones were selected for re-sequencing to provide additional coverage and ensure sequence accuracy. A total of 1,745 unique gene transcripts were identified from the full-length cDNA set, including 1,064 gene transcripts from channel catfish and 681 gene transcripts from blue catfish, with 416 transcripts shared between the two closely related species. Full-length sequence characteristics (ortholog conservation, UTR length, Kozak sequence, and conserved motifs of the channel and blue catfish were examined in detail. Comparison of gene ontology composition between full-length cDNAs and all catfish ESTs revealed that the full-length cDNA set is representative of the gene diversity encoded in the catfish transcriptome. CONCLUSIONS: This study describes the first catfish full-length cDNA set constructed from several cDNA libraries. The catfish full-length cDNA sequences, and data gleaned from sequence characteristics analysis, will be a valuable resource for ongoing catfish whole-genome sequencing and future gene-based studies of function and evolution in teleost fishes.

  3. The Innate Immune-Related Genes in Catfish

    OpenAIRE

    Weidong Liu; Xianggang Gao; Yunfeng Li; Hao Su; Xueguang Liu; Chongbo He; Lei Gao

    2012-01-01

    Catfish is one of the most important aquaculture species in America (as well as in Asia and Africa). In recent years, the production of catfish has suffered massive financial losses due to pathogen spread and breakouts. Innate immunity plays a crucial role in increasing resistance to pathogenic organisms and has generated increasing interest in the past few years. This review summarizes the current understanding of innate immune-related genes in catfish, including pattern recognition receptor...

  4. Welfare of African catfish : effects of stocking density

    OpenAIRE

    Nieuwegiessen, van de, P.G.

    2009-01-01

    The general aim of this thesis was to determine the impact of stocking density on welfare indicators in African catfish, Clarias gariepinus, cultured in a recirculating aquaculture system. The following factors were studied: 1) the effects of stocking density on physical, physiological, and behavioural responses of African catfish, 2) how age mediates the effects of stocking density on physical, physiological, and behavioural responses of African catfish, 3) the potential effects of chemical ...

  5. Comparative Histomorphological Studies on Oesophagus of Catfish and Grass Carp

    OpenAIRE

    Enas A. Abd El Hafez; Doaa M. Mokhtar; Alaa Sayed Abou-Elhamd; Ahmed Hassan S. Hassan

    2013-01-01

    The present work was carried out on 40 specimens of oesophaguses of both sexes of catfish (carnivorous fish) and grass carp (herbivorous fish) in order to observe the morphological and histological differences between the two species. Oesophagus of catfish was divided into 2 parts: anterior and posterior ones. The anterior part of the oesophagus of catfish was characterized by the presence of numerous mucosal folds. It was lined by stratified epithelium with goblet cells. In addition to club ...

  6. Inactivation of Salmonellae in Frozen Catfish by Gamma Irradiation

    International Nuclear Information System (INIS)

    The effect of gamma irradiation on salmonellae viability in frozen catfish was investigated using fresh cut of catfish artificially contaminated with stationary phase cells of salmonellae, frozen at-18 οC and irradiated with does ranging from 0.0 to 2.4 kGy. The D10 values for ten serovars of salmonellae ranged from 0.47 to 0.77 kGy. Salmonella Enteritidis was the most resistant serovars found in frozen catfish. Dosage at 2.5 kGy would be sufficient to kill 103.2 Salmonella Enteritidis that may occasionally present in frozen catfish

  7. Weight-Length Relationships in Gafftopsail Catfish (Bagre marinus) and Hardhead Catfish (Ariopsis felis) in Louisiana Waters

    OpenAIRE

    Courtney, Joshua; Klinkmann, Taylor; Torano, Joseph; 2; Courtney, Michael

    2012-01-01

    In spite of the abundance and commercial importance of these two species, there is little published weight-length data for the gafftopsail catfish (Bagre marinus) and hardhead catfish (Ariopsis felis). For this study 84 catfish were caught (hook and line) from the Calcasieu Estuary in Southwest Louisiana near the Gulf of Mexico and estuaries and near shore waters close to bayou Lafourche. Using least squares regression, best fit curves were determined for weight (W) vs. total length (L) relat...

  8. High-throughput cryopreservation of spermatozoa of blue catfish (Ictalurus furcatus): establishment of an approach for commercial-scale processing

    OpenAIRE

    Hu, E.; Yang, Huiping; Tiersch, Terrence R.

    2010-01-01

    Hybrid catfish created by crossing of female channel catfish (Ictalurus punctatus) and male blue catfish (Ictalurus furcatus) are being used increasingly in foodfish aquaculture because of their fast growth and efficient food conversion. However, the availability of blue catfish males is limited, and their peak spawning is at a different time than that of the channel catfish. As such, cryopreservation of sperm of blue catfish could improve production of hybrid catfish, and has been studied in...

  9. Comparative susceptibility of Channel Catfish, Blue Catfish, and their hybrid cross to experimental challenge with Bolbophorus damnificus (Digenea: Bolbophoridae) cercariae.

    Science.gov (United States)

    Griffin, Matt J; Reichley, Stephen R; Khoo, Lester H; Ware, Cynthia; Greenway, Terrence E; Mischke, Charles C; Wise, David J

    2014-06-01

    The digenetic trematode Bolbophorus damnificus has been implicated in significant losses in catfish aquaculture since the late 1990s. The complex life cycle sequentially involves the American white pelican Pelecanus erythrorhynchos, the marsh rams horn snail Planorbella trivolvis, and Channel Catfish Ictalurus punctatus. Research supports anecdotal reports from the industry, suggesting that the hybrid of Channel Catfish×Blue Catfish I. furcatus is less susceptible to disease agents that have been historically prohibitive to Channel Catfish production, namely the gram-negative bacteria Edwardsiella ictaluri and Flavobacterium columnare, as well as the myxozoan parasite Henneguya ictaluri. This current research compared the susceptibility of Channel Catfish, Blue Catfish, and their hybrid cross to an experimental challenge by B. damnificus. Fish were exposed to 0, 100, 200, and 400 B. damnificus cercariae per fish, and the numbers of metacercariae per fish were determined 14 d postchallenge. Metacercariae were recovered from all challenged fish. There were no significant differences among fish groups challenged with the same dose, suggesting Channel and Blue Catfish and their hybrid are comparably susceptible to B. damnificus infection. As such, it is recommended that producers raising hybrid catfish remain diligent in controlling populations of the snail intermediate host to prevent production losses attributed to B. damnificus, especially when loafing pelicans have been observed at the aquaculture operation. PMID:24895863

  10. Nutritional Regulation of IGFs in Channel Catfish

    Science.gov (United States)

    We examined changes in hepatic IGF-I and IGF-II mRNA, insulin like growth factor binding proteins (IGFBP-1 and IGFBP-2) mRNA, muscle IGF-I and IGF-II mRNA in fed (fed daily for 45 days) and restricted (not fed for 30 days followed by feeding for 15 days) channel catfish. By day 30, liver IGF-I mRNA...

  11. Use of Molecular Markers for Catfish Production and Product Quality

    Science.gov (United States)

    Channel catfish are a diploid species native to North America and the primary product of U.S. aquaculture, and molecular genetic research supports selective breeding of catfish for commercial production. To date, short tandem repeat loci have been used to characterize DNA sequence variation within ...

  12. Production Practices of Commercial Catfish Producers in Northeast Louisiana.

    Science.gov (United States)

    Letlow, Richard M.; Verma, Satish

    A study determined the yields obtained and the production practices followed by Louisiana commercial catfish farmers in Catahoula, Concordia, and Franklin Parishes. This information was used by the Louisiana Cooperative Extension Service (LCES) faculty to develop a catfish education program. The study: (1) determined use of selected recommended…

  13. A liquid oxygen calculator for fasted channel catfish

    Science.gov (United States)

    A review of scientific literature concerning channel catfish Ictalurus punctatus respiration resulted in development of a Microsoft Excel© spreadsheet for estimating the volume of oxygen consumed by a given fasted channel catfish biomass. Entry of ten variables into the spreadsheet provides estimate...

  14. Stress in African catfish (clarias gariepinus) following overland transportation

    NARCIS (Netherlands)

    Manuel, R.; Boerrigter, J.; Roques, J.; Heul, van der J.W.; Bos, van den R.; Flik, G.; Vis, van de J.W.

    2014-01-01

    Of the many stressors in aquaculture, transportation of fish has remained poorly studied. The objective of this study was therefore to assess the effects of a (simulated) commercial transportation on stress physiology of market-size African catfish (Clarias gariepinus). Catfish weighing approximatel

  15. Safety of copper sulfate to channel catfish eggs

    Science.gov (United States)

    Copper sulfate (CuSO4) is commonly used in the catfish industry to control saprolegniasis (caused by watermolds) on eggs. This study was designed to establish the safety of CuSO4 when applied to hatching troughs containing channel catfish eggs in 26 degrees C flow-through well water at 10, 30, and ...

  16. Cannibalism in single-batch hybrid catfish production ponds

    Science.gov (United States)

    Hybrid catfish are more efficiently harvested by seining than are Channel Catfish. Due to that, and their faster growth, hybrids are typically produced in “single-batch” production systems, either in intensively-aerated commercial ponds or in split-pond systems. In either production system, hybrids...

  17. Pre-spawning carotenoid fortified diets improve reproductive traits of channel catfish, Ictalurus punctatus and subsequent progeny performance

    Science.gov (United States)

    Availability of consistent number of ovulatory competent channel catfish females is a pre-requisite for efficient production of channel catfish ' x blue catfish, I.furcatus ') hybrid in hatcheries. Raising hybrid catfish in production ponds enables the catfish farmer to harness improved growth rate...

  18. Changes in Price Behavior in the U.S. Catfish Industry: Evidence Using Cointegration

    OpenAIRE

    Hudson, Darren

    1998-01-01

    The implications of market development in the catfish industry on catfish price behavior are explored using cointegration. It is hypothesized that market development, through increases in competition between processors and shifts in consumer preferences toward fish, has caused changes in price behavior among levels of the catfish market. Using monthly catfish price data, a cointegration analysis of subsets of prices shows that price behavior has changed through time, with catfish prices becom...

  19. Reductions in Susceptibility of Channel Catfish (Ictalutus punctatus) to Enteric Septicemia of Catfish (ESC) Through Two Generations of Selection

    Science.gov (United States)

    Improvement of disease resistance in aquaculture species through selective breeding has had some success. At the USDA-ARS Catfish Genetics Research Unit, Stoneville, MS, a multitrait selection index was utilized to select for growth, carcass yield, and resistance to enteric septicemia of catfish (...

  20. 76 FR 26655 - Public Meetings on the Proposed Rule for Mandatory Inspection of Catfish and Catfish Products

    Science.gov (United States)

    2011-05-09

    ... meetings, interested persons may submit comments on the proposed rule (76 FR 10434) on or before June 24..., 552, 555, 557, and 559-561 Public Meetings on the Proposed Rule for Mandatory Inspection of Catfish and Catfish Products AGENCY: Food Safety and Inspection Service, USDA. ACTION: Notice of...

  1. First Season Catfish Farming. A Workbook for Beginning Pond and Cage Culture of Channel Catfish. Teacher Edition and Student Edition.

    Science.gov (United States)

    Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This workbook, comprised of both the teacher and student editions, presents guidelines useful for first-year catfish farmers in Oklahoma using pond or cage cultures to raise channel catfish. The teacher edition is a set of unit guidelines only. Contents include a list of suggested readings, important addresses with types of information available…

  2. Production of channel catfish and channel x blue hybrid catfish subjected to two minimum dissolved oxygen concentrations

    Science.gov (United States)

    As the channel x blue hybrid catfish is stocked by an increasing number of catfish farmers, it is important to quantify the production response of this fish to dissolved oxygen management strategies. The purpose of this study was to compare the production and water quality responses of the channel x...

  3. Oral vaccination of channel catfish against enteric septicemia of catfish (ESC) using a live attenuated Edwardsiella ictaluri isolate

    Science.gov (United States)

    Enteric septicemia of catfish (ESC), caused by Edwardsiella ictaluri, is the most problematic bacterial disease affecting catfish aquaculture in the southeastern United States. Efforts to develop an effective ESC vaccine have had limited industrial success. In commercial settings, ESC vaccines are t...

  4. Oral vaccination of channel catfish against enteric septicemia of catfish using a live attenuated Edwardsiella ictaluri isolate

    Science.gov (United States)

    Enteric septicemia of catfish (ESC), caused by Edwardsiella ictaluri, is the most problematic bacterial disease affecting catfish aquaculture in the southeastern United States. Efforts to develop an effective ESC vaccine have had limited industrial success. In commercial settings, ESC vaccines are...

  5. Chemical composition and antibacterial activity of Aloysia triphylla (L'Hérit Britton extracts obtained by pressurized CO2 extraction

    Directory of Open Access Journals (Sweden)

    Thaylise Vey Parodi

    2013-04-01

    Full Text Available This study investigated the chemical composition of five different extracts of Aloysia triphylla and their activity against Aeromonas sp. The extracts were obtained from the dried leaves by pressurized CO2 extraction at 30, 50 and 70ºC, and 100, 150, and 200 bar, and analyzed by GC/FID and GC-MS. The antibacterial activity was assayed by the microdilution method. The tested microorganisms comprised seven Aeromonas isolates obtained from the kidney of infected silver catfish, Rhamdia quelen. The yield, chemical composition and antibacterial activity of the extracts were dependent on the extraction conditions. Mono and sesquiterpenoids were the major constituents of all the extracts and the highest extraction yield was obtained at 70ºC and 200 bar. A. triphylla presented moderate antibacterial activity against Aeromonas sp.

  6. "No evidence for intercohort cannibalism in mixed-size cultures of food-size and fingerling hybrid catfish (channel catfish x blue catfish) grown in ponds in winter or summer."

    Science.gov (United States)

    Hybrid catfish (' Channel Catfish Ictalurus punctatus X ' Blue Catfish I. furcatus) are normally harvested by seining single-batch pond production systems in fall or winter. Ponds are typically restocked without draining. There is concern that without completely draining the pond after harvest, food...

  7. Comparative Histomorphological Studies on Oesophagus of Catfish and Grass Carp

    Directory of Open Access Journals (Sweden)

    Enas A. Abd El Hafez

    2013-01-01

    Full Text Available The present work was carried out on 40 specimens of oesophaguses of both sexes of catfish (carnivorous fish and grass carp (herbivorous fish in order to observe the morphological and histological differences between the two species. Oesophagus of catfish was divided into 2 parts: anterior and posterior ones. The anterior part of the oesophagus of catfish was characterized by the presence of numerous mucosal folds. It was lined by stratified epithelium with goblet cells. In addition to club cells were observed in between the stratified epithelium. Scanning electron examination of the oesophageal epithelium of catfish demonstrated the presence of microvilli and fingerprint-like microridges in the superficial cell layer. The posterior part of the oesophagus of catfish was characterized by simple columnar mucus-secreting epithelium. The oesophagus of grass carp had shown the same structure along its entire length. It consisted of less folded mucosa than that observed in the oesophagus of catfish. The epithelium was characterized by the presence of taste buds. In conclusion, the present work revealed some differences in the structure of catfish oesophagus and grass carp oesophagus. These differences are related to type of food and feeding habits of each species.

  8. An AFLP-based genetic linkage map of channel catfish (Ictalurus punctatus) constructed by using an interspecific hybrid resource family.

    OpenAIRE

    Liu, Zhanjiang; Karsi, Attila; Li, Ping; Cao, Dongfeng; Dunham, R

    2003-01-01

    Catfish is the major aquaculture species in the United States. The hybrid catfish produced by crossing channel catfish females with blue catfish males exhibit a number of desirable production traits, but their mass production has been difficult. To introduce desirable genes from blue catfish into channel catfish through introgression, a genetic linkage map is helpful. In this project, a genetic linkage map was constructed using amplified fragment length polymorphism (AFLP). A total of 607 AFL...

  9. The Impact of Catfish Imports on the U.S. Wholesale and Farm Sectors

    OpenAIRE

    Muhammad, Andrew; Neal, Sammy J.; Hanson, Terrill R.; Jones, Keithly G.

    2010-01-01

    The primary objective of this study was to assess the impact of catfish imports and tariffs on the U.S. catfish industry, with particular focus on the U.S. International Trade Commission ruling on Vietnam in 2003. Given the importance of Vietnam to the U.S. catfish market, it was assumed that catfish import prices would increase by 35 percent if the maximum tariff was imposed on catfish from Vietnam. With the tariff, domestic catfish prices at the wholesale level would increase by $0.06 per l...

  10. Identification and Characterization of Full-Length cDNAs in Channel Catfish (Ictalurus punctatus) and Blue Catfish (Ictalurus furcatus)

    OpenAIRE

    Chen, Fei; Lee, Yoona; Jiang, Yanliang; Wang, Shaolin; Peatman, Eric; Abernathy, Jason; Liu, Hong; Liu, Shikai; Kucuktas, Huseyin; Ke, Caihuan; Liu, Zhanjiang

    2010-01-01

    Background Genome annotation projects, gene functional studies, and phylogenetic analyses for a given organism all greatly benefit from access to a validated full-length cDNA resource. While increasingly common in model species, full-length cDNA resources in aquaculture species are scarce. Methodology and Principal Findings Through in silico analysis of catfish (Ictalurus spp.) ESTs, a total of 10,037 channel catfish and 7,382 blue catfish cDNA clones were identified as potentially encoding f...

  11. Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

    Science.gov (United States)

    The mucosal barriers of catfish (Ictalurus spp.) constitute the first line of defense against pathogen invasion while simultaneously carrying out a diverse array of other critical physiological processes, including nutrient adsorption, osmoregulation, waste excretion, and environmental sensing. Catf...

  12. The Innate Immune-Related Genes in Catfish

    Directory of Open Access Journals (Sweden)

    Weidong Liu

    2012-11-01

    Full Text Available Catfish is one of the most important aquaculture species in America (as well as in Asia and Africa. In recent years, the production of catfish has suffered massive financial losses due to pathogen spread and breakouts. Innate immunity plays a crucial role in increasing resistance to pathogenic organisms and has generated increasing interest in the past few years. This review summarizes the current understanding of innate immune-related genes in catfish, including pattern recognition receptors, antimicrobial peptides, complements, lectins, cytokines, transferrin and gene expression profiling using microarrays and next generation sequencing technologies. This review will benefit the understanding of innate immune system in catfish and further efforts in studying the innate immune-related genes in fish.

  13. Isolation and characterization of Vibrio ( Listonella) anguillarum from catfish

    OpenAIRE

    Rad, Mehrnaz; Shahsavani, Davar

    2010-01-01

    Vibrio anguillarum was isolated from several organs of some aquarium catfish in a fish population with high mortality. Macroscopic examination of the affected catfish revealed ascites in the abdomen, site-petechiae, and dorsal erection. Although there are some variations in phenotypic characterization among different isolates of Vibrio anguillarum, the majority show a common biochemical profile with distinct microscopic appearance that can provide presumptive identification of Vibrio anguilla...

  14. Identification of Bacillus Strains for Biological Control of Catfish Pathogens

    OpenAIRE

    Ran, Chao; Carrias, Abel; Williams, Malachi A.; Capps, Nancy; Dan, Bui C. T.; Newton, Joseph C.; Joseph W Kloepper; Ooi, Ei L.; Browdy, Craig L.; Terhune, Jeffery S.; Liles, Mark R.

    2012-01-01

    Bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against Edwardsiella ictaluri and Aeromonas hydrophila, the causative agents of enteric septicemia of catfish (ESC) and motile aeromonad septicaemia (MAS), respectively. Twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. Each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including...

  15. Xeroradiographic and radiographic anatomy of the channel catfish, Ictalurus punctatus

    International Nuclear Information System (INIS)

    The purpose of this study was to provide an anatomic reference for the channel catfish (Ictalurus punctatus) using xeroradiography† and conventional radiography. The entire body of three adult fish was radiographed using standard xeroradiographic and conventional radiographic techniques. Two xeroradiographs and their corresponding conventional radiographs were selected, and the xeroradiographs labeled to illustrate the normal skeletal and soft-tissue anatomy of the channel catfish

  16. Price Volatility Spillover in Agricultural Markets: An Examination of U.S. Catfish Markets

    OpenAIRE

    Buguk, Cumhur; Hudson, Darren; Hanson, Terrill R.

    2003-01-01

    Price volatility spillovers in the U.S. catfish supply chain are analyzed based on monthly price data from 1980 through 2000 for catfish feed, its ingredients, and farm- and wholesale-level catfish. The exponential generalized autoregressive conditional heteroskedasticity (EGARCH) model was used to test univariate volatility spillovers for prices in the supply chain. Strong price volatility spillover from feeding material (corn, soybeans, menhaden) to catfish feed and farm- and wholesale-leve...

  17. EFFECTS OF GENERIC ADVERTISING ON PERCEPTIONS AND BEHAVIOR: THE CASE OF CATFISH

    OpenAIRE

    Kinnucan, Henry W.; Venkateswaran, Meenakshi

    1990-01-01

    An eight equation partially-recursive econometric model is specified to indicate the effects of catfish advertising on product awareness, beliefs, attitude and consumption. Results indicate that the ad campaign in its first year (i) increased consumer's awareness on farm-raised catfish 15 percent, (ii) improved consumers' perceptions of and attitude toward catfish 3 to 6 percent, and (iii) increased at-home and restaurant purchases of catfish 12 to 13 percent. The response to the ad campaign ...

  18. 40 CFR 408.10 - Applicability; description of the farm-raised catfish processing subcategory.

    Science.gov (United States)

    2010-07-01

    ...-raised catfish processing subcategory. 408.10 Section 408.10 Protection of Environment ENVIRONMENTAL... SOURCE CATEGORY Farm-Raised Catfish Processing Subcategory § 408.10 Applicability; description of the farm-raised catfish processing subcategory. The provisions of this subpart are applicable to...

  19. Hydrogen peroxide treatments for channel catfish eggs infected with water molds

    Science.gov (United States)

    Fungi, or water molds Saprolegnia spp., on channel catfish Ictalurus punctatus eggs can lower fry production. This requires the producer to spawn more catfish or face fingerling shortages. Few treatments have been tested against channel catfish eggs infested with an identified fungus. Hydrogen pe...

  20. Proximate Composition and Collagen Concentration of Processing Residue of Channel Catfish

    Science.gov (United States)

    Residues (including heads, skin, viscera, frames, and trimmings) from the channel catfish Ictalurus punctatus processing industry have generally been made into catfish meal and catfish oil that are used in animal feeds. There may be more efficient uses for these materials, such as producing collagen...

  1. Breeding strategy of US farm-raised channel catfish, Ictalurus punctatus affects progeny production and performance

    Science.gov (United States)

    catfish propagation for decades has been dependent on random mating of male and female channel catfish in ponds. It is simple and has been fairly successful in fulfilling the needs of the US farm-raised catfish industry. However, natural pond spawning is unreliable, unpredictable, and incurs 30 t...

  2. SNP discovery and validation in wild and domesticated populations of blue catfish, Ictalurus furcatus, using genotyping-by-sequencing (GBS)

    Science.gov (United States)

    Blue catfish, Ictalurusfurcatus, are valued in the United States as a trophy fishery for their capacity to reach large sizes, sometimes exceeding 45 kg. Additionally blue catfish x channel catfish (I. punctatus) hybrid food fish production has recently increased the demand for blue catfish broodsto...

  3. Suspending mammalian LHRHa-injected channel catfish, Ictalurus punctatus in individual nylon-mesh bag reduces stress and improves reproductive performance

    Science.gov (United States)

    Hormone induced spawning of channel catfish held communally in tanks is a reliable method to produce channel catfish, Ictalurus punctatus ' x blue catfish, I. furcatus ' F1 hybrid catfish fry. However, mature catfish are crowded, and repeatedly handled during the process of induced ovulation. Repe...

  4. High-density interspecific genetic linkage mapping provides insights into genomic incompatibility between channel catfish and blue catfish.

    Science.gov (United States)

    Liu, S; Li, Y; Qin, Z; Geng, X; Bao, L; Kaltenboeck, L; Kucuktas, H; Dunham, R; Liu, Z

    2016-02-01

    Catfish is the leading aquaculture species in the United States. The interspecific hybrid catfish produced by mating female channel catfish with male blue catfish outperform both of their parent species in a number of traits. However, mass production of the hybrids has been difficult because of reproductive isolation. Investigations of genome structure and organization of the hybrids provide insights into the genetic basis for maintenance of species divergence in the face of gene flow, thereby helping develop strategies for introgression and efficient production of the hybrids for aquaculture. In this study, we constructed a high-density genetic linkage map using the hybrid catfish system with the catfish 250K SNP array. A total of 26,238 SNPs were mapped to 29 linkage groups, with 12,776 unique marker positions. The linkage map spans approximately 3240 cM with an average intermarker distance of 0.25 cM. A fraction of markers (986 of 12,776) exhibited significant deviation from the expected Mendelian ratio of segregation, and they were clustered in major genomic blocks across 15 LGs, most notably LG9 and LG15. The distorted markers exhibited significant bias for maternal alleles among the backcross progenies, suggesting strong selection against the blue catfish alleles. The clustering of distorted markers within genomic blocks should lend insights into speciation as marked by incompatibilities between the two species. Such findings should also have profound implications for understanding the genomic evolution of closely related species as well as the introgression of hybrid production programs in aquaculture.

  5. EVALUATION OF THE POSSIBLE THREAT OF NAFTA ON U.S. CATFISH INDUSTRY USING A TRADITIONAL IMPORT DEMAND FUNCTION

    OpenAIRE

    Ligeon, Carel; Jolly, Curtis M.; John D. Jackson

    1996-01-01

    The effects of increased exports from NAFTA member countries on the U.S. domestic catfish industry were evaluated. Results showed that the quantity of catfish imported will fall if the domestic price of catfish falls relative to the import price. Past imports have no effect on present imports. The income elasticity was negative indicating that imported catfish may be an inferior good. Doubling present levels of imports from NAFTA member countries is not a threat to the U.S. catfish industry.

  6. ANALYSIS OF FACTORS INFLUENCING THE FREQUENCY OF CATFISH CONSUMPTION IN THE UNITED STATES

    OpenAIRE

    Drammeh, Lamin; House, Lisa; Sureshwaran, Suresh; Selassie, Haile

    2002-01-01

    Consumption of seafood, including catfish, has become an important part of the diet for consumers in the United States. Per capita consumption of catfish increased from 0.41 pounds in 1985 to 0.90 pounds in 2001. The goal of this study is to investigate factors that influence the decisions to consume and frequency of consumption of catfish. One finding was the main emphasis of the Catfish Institute to market catfish as "farm-raised" and to increase preparation knowledge through distribution o...

  7. Population characteristics of channel catfish near the northern edge of their distribution: implications for management

    Science.gov (United States)

    Carter-Lynn, K. P.; Quist, Michael

    2015-01-01

    Channel catfish, Ictalurus punctatus (Rafinesque), populations in six lakes in northern Idaho, USA, were sampled to describe their population characteristics. During the summers of 2011 and 2012, 4864 channel catfish were sampled. Channel catfish populations had low to moderate catch rates, and length structure was dominated by fish body condition. All populations were maintained by stocking age-1 or age-2 fish. Growth of fish reared in thermally enriched environments prior to stocking was fast compared to other North American channel catfish populations. After stocking, growth of channel catfish declined rapidly. Once stocked, cold water temperatures, prey resources and (or) genetic capabilities limited growth. Total annual mortality of age 2 and older channel catfish was generally factors regulating channel catfish population dynamics and highlights important considerations associated with their ecology and management.

  8. Book review: Conservation, ecology, and management of catfish: The second international symposium

    Science.gov (United States)

    Long, James M.

    2013-01-01

    This is the second publication in a series published by the American Fisheries Society devoted to catfish research, biology, and management, which was organized as a symposium held in St. Louis Missouri, in 2010 and builds upon the first symposium that was held in Davenport, Iowa, in 1998 (Irwin et al. 1999).  The organizations of the second symposium stated that this was to "serve as an addendum to Catfish 2000" and "to cover the four corners of catfish science: catfish biology, ecology, management, and conservation" (p. xi).  The book meets this standard easily.  With 64 pages organized into nine sections (plenary, catfishes as sport fish, non-game catfishes, nonnative catfishes, movement and habitat use, sampling and population assessment, age and growth, behavior, and future directions), this publication covers these four corners and more.

  9. Evaluation of Zooplankton in Hatchery Diets for Channel Catfish Fry

    Science.gov (United States)

    The efficacy of zooplankton as a supplemental hatchery diet for fry of channel catfish Ictalurus punctatus was evaluated. When a commercial diet is used as a reference, fry fed exclusively on zooplankton–either live or dried–performed poorly in their growth rate. However, when live or dried zooplan...

  10. Temperature Cycles Induce Early Maturation in Channel Catfish

    Science.gov (United States)

    A major impediment in improvement of channel catfish by selective breeding is that a high percent of fish do not spawn until the third year. If the generation time could be shortened, genetic improvement could be achieved at a faster rate. The conditions that lead to sexual maturation in fish have...

  11. Delayed feeding of channel catfish fry stocked in ponds

    Science.gov (United States)

    We compared production variables between channel catfish, Ictalurus punctatus, nursery ponds fed according to industry standards, that is feeding immediately at stocking, to an alternative practice of delaying feeding for 6 wk after stocking in an effort to utilize natural pond productivity and redu...

  12. TOXAPHENE: CHRONIC TOXICITY TO FATHEAD MINNOWS AND CHANNEL CATFISH

    Science.gov (United States)

    Fathead minnows (Pimephales promelas) and channel catfish (Ictalurus punctatus) were continuously exposed to several toxaphene concentrations (13-630 ng) in flow-through diluter systems for 8 to 10 months. Growth and backbone quality of adult fathead minnows were decreased at 97 ...

  13. Can We Improve Catfish Growth and FCR Through Oxygen Management?

    Science.gov (United States)

    Studies were conducted in 15 1-acre and six ¼-acre ponds over several years to determine the effect of low dissolved oxygen (DO) concentration on food conversion ratio (FCR), food consumption, growth, and net production of channel catfish. Control ponds in each study were maintained with a minimum D...

  14. Channel Catfish, Ictalurus punctatus, CD63 cDNA

    Science.gov (United States)

    CD63, also known as lysosome associated membrane protein 3 (LAMP-3), is a member of tetraspanin integral membrane protein family. This protein plays many important roles in immuno-physiological functions. In this communication, we cloned, sequenced and characterized the channel catfish, CD63 transcr...

  15. On Sciadeichthys (Selenaspis) Walrechti, a new South American catfish

    NARCIS (Netherlands)

    Boeseman, M.

    1954-01-01

    Two large specimens of a hitherto unknown species of neotropical catfish have recently been found dead and washed ashore beneath the dike along the Westerscheldt near Biezelinge, Zeeland, at a mutual distance of approximately 300 meters. Both were in excellent condition and have been presented to th

  16. Welfare of African catfish : effects of stocking density

    NARCIS (Netherlands)

    Nieuwegiessen, van de P.G.

    2009-01-01

    The general aim of this thesis was to determine the impact of stocking density on welfare indicators in African catfish, Clarias gariepinus, cultured in a recirculating aquaculture system. The following factors were studied: 1) the effects of stocking density on physical, physiological, and behaviou

  17. Semen collection and preservation in African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Viveiros, A.T.M.

    2002-01-01

    Stock improvement using quantitative and molecular genetics is an essential part of nowadays production of farm animals and fish. To achieve this in aquaculture, germplasm of both parental sexes should be obtained in a life-saving manner. In captivity, male African catfish, Clariasgariepin

  18. Evaluation of locally available feed resources for striped catfish (Pangasianodon hypophthalmus)

    OpenAIRE

    Da, Chau Thi

    2012-01-01

    This thesis investigated and compared inputs and outputs, economic factors and current feed use in small-scale farming systems producing striped catfish (Pangasianodon hypophthalmus) in the Mekong Delta. The nutrient content of locally available natural feed resources for striped catfish was determined and growth performance, feed utilisation and body indices were analysed in pond-cultured striped catfish fed diets where fish meal protein was replaced with protein from local feed resources. ...

  19. Analysis of a Blue Catfish Population in a Southeastern Reservoir: Lake Norman, North Carolina

    OpenAIRE

    Grist, Joseph Daniel

    2002-01-01

    This investigation examined the diet, growth, movement, population genetics, and possible consumption demands of an introduced blue catfish Ictalurus furcatus population in Lake Norman, North Carolina. Clupeids, Corbicula fluminea, and Chara were the predominant food items (percent stomach contents by weight) found in blue catfish, and varied by season, lake-region, and fish size-class. Lake Norman blue catfish grow at a slower rate than has been reported for other reservoir populations, wi...

  20. Genomic organization of the channel catfish CD45 functional gene and CD45 pseudogenes

    OpenAIRE

    Kountikov, Evgueni; Wilson, Melanie; Miller, Norman; Clem, William; Bengtén, Eva; Quiniou, Sylvie

    2005-01-01

    CD45 is a transmembrane protein tyrosine phosphatase, which in mammals plays an important role in T and B cell receptor and cytokine signaling. Recently, a catfish cDNA was shown to contain all characteristic CD45 features: an alternatively spliced amino-terminus, a cysteine-rich region, three fibronectin domains, a transmembrane region, and two phosphotyrosine phosphatase domains. However, analyses of CD45 cDNAs from various catfish lymphoid cell lines demonstrated that catfish CD45 is uniqu...

  1. INTRA-PROCESSOR PRICE-SPREAD BEHAVIOR: IS THE U.S. CATFISH PROCESSING INDUSTRY COMPETITIVE?

    OpenAIRE

    Hudson, Darren

    1998-01-01

    An analysis was conducted of price-spread behavior in the catfish-processing sector of the United States. A model of imperfect competition using conjectural variations was used to test for significant deviations from competition. Results show no significant deviation from competitive behavior, suggesting that catfish processor behave competitively. However, this result is limited by the assumption of equal market shares by each catfish-processing firm.

  2. IQF Catfish Retail Pack: A Study of Consumers' Willingness to Pay

    OpenAIRE

    Quagrainie, Kwamena K.

    2005-01-01

    The grocery retail channel represents a potential for increased sales for catfish products because of the competitive nature that imported catfish fillets pose at foodservice market channels. The study examined the potential for selling a household-size pack of IQF 6-fillets of catfish through the grocery market channels, and consumers' willingness to pay for the product. Data used were obtained from a survey conducted in selected southern U.S. cities. Results suggest that households will pur...

  3. Adjusting to Trade Policy: Evidence from U.S. Antidumping Duties on Vietnamese Catfish

    OpenAIRE

    Irene Brambilla; Guido Porto; Alessandro Tarozzi

    2008-01-01

    In 2003, after claims of dumping, the U.S. imposed heavy tariffs on imports of catfish from Vietnam. As a result, Vietnamese exports of catfish to the U.S. market sharply declined. Using a panel data of Vietnamese households, we explore the responses of catfish producers in the Mekong delta between 2002 and 2004. We study adjustments not only in catfish aquaculture but also in other economic activities. We find that, over this period, the rate of income growth was significantly lower among ho...

  4. Effects of Endosulfan on Predator–Prey Interactions Between Catfish and Schistosoma Host Snails

    OpenAIRE

    Monde, Concillia; Syampungani, Stephen; Brink, van den, L.

    2016-01-01

    The effect of the pesticide endosulfan on predator–prey interactions between catfish and Schistosoma host snails was assessed in static tank experiments. Hybrid catfish (Clarias gariepinus × C. ngamensis) and Bulinus globosus were subjected to various endosulfan concentrations including an untreated control. The 48- and 96-h LC50 values for catfish were 1.0 and 50 values for snails were 1137 and 810 µg/L. To assess sublethal effects on the feeding of the catfish on B. globosus, endosulfan con...

  5. Dynamic Effects of Grain and Energy Prices on the Catfish Feed and Farm Sectors

    OpenAIRE

    Muhammad, Andrew; Zheng, Hualu

    2010-01-01

    This study examines the dynamic effects of grain prices and energy prices on catfish feed prices and the price of food-sized catfish at the farm level. Using the autoregressive distributed lag model and bounds testing procedure, a long-run relationship between feed and farm prices and their determinants was confirmed. Given the effect of corn and soybean meal prices on catfish feed prices, and catfish fish feed prices on farm prices, the long-run responsiveness of feed prices to a percentage ...

  6. BOB.1 of the channel catfish, Ictalurus punctatus: Not a transcriptional coactivator?

    OpenAIRE

    Richard, Mara L. Lennard; Hikima, Jun-ichi; Wilson, Melanie R.; Miller, Norman W.; Cunningham, Charles; Warr, Gregory W.

    2008-01-01

    Expression of the immunoglobulin heavy chain (IGH) locus of the channel catfish (Ictalurus punctatus)is driven by the Eμ3′ enhancer, whose core region contains two octamer motifs and a μE5 site. Orthologues of the Oct1 and Oct2 transcription factors have been cloned in the channel catfish and shown to bind to the octamer motifs within the core enhancer. While catfish Oct2 is an activator of transcription, catfish Oct1 failed to drive transcription and may act as a negative regulator of IGH tr...

  7. Factors affecting the decision process of catfish consumers: An empirical study in the two biggest cities in Vietnam

    OpenAIRE

    Huynh, Mai Thi Xuan

    2007-01-01

    The consumption of catfish in Vietnam has not grown enough to match their increased production. The objective of this study is to explore the factors that influence the decision process of catfish consumers in Vietnam. The findings provide information for the Vietnamese catfish industry to better attract more domestic consumers to eat catfish. This study seeks to address its objective by examining the relationships that exist between the experiences, perceptions of product attr...

  8. Comprehensive survey and genomic characterization of toll-like receptors in channel catfish, Ictalurus punctatus: identification of novel fish TLRs

    Science.gov (United States)

    A comprehensive survey of channel catfish Toll-Like Receptors (TLRs) was undertaken following a genomic PCR approach based on degenerate primers. Twenty different TLRs were identified in channel catfish. Channel catfish TLR sequences were characterized by phylogenetic analysis based on their conserv...

  9. Seasonal Differences in Steroids and Maturation-related Genes in Channel Catfish Under Normal and Accelerated Thermoperiods

    Science.gov (United States)

    Selective breeding of channel catfish, Ictalurus punctatus, is hampered by a long generation time. Female channel catfish typically spawn when they are 3-years-old; however, a low percentage of spawning may be observed at two years of age. Mature female channel catfish can spawn once annually. Their...

  10. In vivo and in vitro CYP1B mRNA expression in channel catfish.

    Science.gov (United States)

    Willett, Kristine L; Ganesan, Shobana; Patel, Monali; Metzger, Christine; Quiniou, Sylvie; Waldbieser, Geoff; Scheffler, Brian

    2006-07-01

    Our goal was to study the induction of CYP1B mRNA expression in channel catfish (Ictalurus punctatus). CYP1B belongs to the cytochrome P450 superfamily of genes, is involved in the oxidation of endogenous and exogenous compounds, and could potentially be a useful biomarker in fish for exposure to AhR ligands. The full-length catfish CYP1B cDNA is 2417 nt to the polyA tail and encodes a putative protein of 536 amino acids. It has 67% amino acid similarity to carp and zebrafish CYP1B and 68% similarity to carp CYP1B2. Male channel catfish were collected from three Mississippi Delta sites: Lake Roebuck, Itta Bena; Bee Lake, Thornton; and Sunflower River, Indianola. Total RNA was isolated from wild-caught catfish gill, blood, gonad and liver tissues. Quantitative real-time reverse transcriptase PCR was used to determine relative induction of CYP1B in wild catfish compared to laboratory control and BaP-exposed catfish (20mg/kg i.p. after 4 days). BaP exposure significantly induced CYP1B message in blood, gonad, and liver of laboratory catfish. In these same tissues of wild catfish from sites with relatively low sediment contaminants, CYP1B message was not statistically increased relative to laboratory control catfish. CYP1B transcript abundance was higher in gills compared to other tissues in both laboratory and wild catfish. When primary cultured gill cells were treated with increasing concentrations of BaP, TCDD, and PCBs 77, 126 and 169, CYP1B mRNA was induced more than 10-fold while PCB153 and 4,4'DDT did not cause significant CYP1B induction. Our results suggest that catfish CYP1B is induced by the classic AhR ligands. PMID:16697458

  11. An evaluation of agreement between pectoral spines and otoliths for estimating ages of catfishes

    Science.gov (United States)

    Olive, J.A.; Schramm, Harold; Gerard, Patrick D.; Irwin, E.

    2011-01-01

    Otoliths have been shown to provide more accurate ages than pectoral spine sections for several catfish populations; but sampling otoliths requires euthanizing the specimen, whereas spines can be sampled non-lethally. To evaluate whether, and under what conditions, spines provide the same or similar age estimates as otoliths, we examined data sets of individual fish aged from pectoral spines and otoliths for six blue catfish Ictalurus furcatus populations (n=420), 14 channel catfish Ictalurus punctatus populations (n=997), and 10 flathead catfish Pylodictus olivaris populations (n=947) from lotic and lentic waters throughout the central and eastern U.S. Logistic regression determined that agreement between ages estimated from otoliths and spines was consistently related to age, but inconsistently related to growth rate. When modeled at mean growth rate, we found at least 80% probability of no difference in spine- and otolith-assigned ages up to ages 4 and 5 for blue and channel catfish, respectively. For flathead catfish, an 80% probability of agreement between spine- and otolith-assigned ages did not occur at any age due to high incidence of differences in assigned ages even for age-1 fish. Logistic regression models predicted at least 80% probability that spine and otolith ages differed by ≤1 year up to ages 13, 16, and 9 for blue, channel, and flathead catfish, respectively. Age-bias assessment found mean spine-assigned age differed by less than 1 year from otolith-assigned age up to ages 19, 9, and 17 for blue catfish, channel catfish, and flathead catfish, respectively. These results can be used to help guide decisions about which structure is most appropriate for estimating catfish ages for particular populations and management objectives.

  12. Efficiency of baited hoop nets for sampling catfish in southeastern U.S. small impoundments

    Science.gov (United States)

    Wallace, Benjamin C.; Weaver, Daniel M.; Kwak, Thomas J.

    2011-01-01

    Many U.S. natural resource agencies stock catfish (Ictaluridae) into small impoundments to provide recreational fishing opportunities. However, effective standardized methods for sampling catfish in small impoundments have not been developed for wide application, particularly in the southeastern United States. We evaluated the efficiency of three bait treatments (i.e., soybean cake, sunflower cake, and no bait) of tandem hoop nets in two North Carolina small impoundments during the fall of 2008 and spring of 2009 in a factorial experimental design. The impoundments were stocked with catchable-size channel catfish Ictalurus punctatus at contrastingly low (5.5 fi sh/ha) and high (90.0 fi sh/ha) rates prior to our sampling. Nets baited with soybean cake consistently sampled more channel catfish than any other treatment. Channel catfish catch ranged as high as 3,251 fi sh per net series during the fall in nets baited with soybean cake in the intensively stocked impoundment and was up to 8.5 and 15.3 times higher during the fall than in the spring in each impoundment. Nets baited with soybean cake sampled significantly (12 and 24 times) more channel catfish than those with no bait in the two impoundments. These trends did not occur among other catfish species. Nonictalurid fish and turtle catch was higher during spring compared to that of fall, corresponding with low channel catfish catches. Our results indicate that tandem hoop nets baited with soybean cake during the fall is a more efficient method for sampling channel catfish compared to nets baited with sunflower cake or no bait in spring or fall. Our findings validate this technique for application in southeastern U.S. small impoundments to assess catfish abundance to guide management and evaluate the success of catfish stocking programs.

  13. DNA damage and radiocesium in channel catfish from Chernobyl

    International Nuclear Information System (INIS)

    The explosion of the Chernobyl Nuclear Power Plant resulted in some of the most radioactively contaminated habitats on earth. Despite evacuation of all human inhabitants from the most contaminated areas, animals and plants continue to thrive in these areas. This study examines the levels of contamination and genetic damage associated with cesium-137 in catfish (Ictalurus punctatus) from the cooling pond and a control site. In general, catfish from the cooling pond exhibit greater genetic damage, and the amount of damage is related to the concentration of radiocesium in individual fish. Genetic damage is primarily in the form of DNA strand breaks, with few micronuclei being observed in contaminated fish. The possible roles that acclimation and adaption play in the response to high levels of radiation exposure are discussed

  14. Complete mitochondrial genome of catfish Eutropiichthys vacha (Hamilton, 1822).

    Science.gov (United States)

    Punhal, Lashari; Laghari, Muhammad Younis; Waryani, Baradi; Jalbani, Shaista; Narejo, Naeem Tariq; Zhang, Yan

    2016-09-01

    Complete mitochondrial genome of catfish, Eutropiichthys vacha, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome, which is listed Critically Endangered and Red-listed species. The complete mitogenome was 16,478 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 31.06% A, 27.59% C, 15.65% G, and 25.68% T. The complete mitochondrial genome of catfish, E. vacha provides the fundamental tool for genetic breeding and conservation studies. PMID:25630731

  15. Production of enzymatic protein hydrolysates from freshwater catfish (Clarias batrachus)

    Science.gov (United States)

    Seniman, Maizatul Sarah Md; Yusop, Salma Mohamad; Babji, Abdul Salam

    2014-09-01

    Fish protein hydrolysate (FPH) was prepared from freshwater catfish (Clarias batrachus) by using Alcalase® 2.4L and Papain. The effect of hydrolysis time (30, 60, 120, 180 min) with enzyme concentration of 1% (v/w substrate); pH = 8.0, 7.0 was studied to determine the degree of hydrolysis (DH), peptide content, proximate composition and amino acid profile. Results showed that the highest DH of Alcalase and Papain FPH were 58.79% and 53.48% after 180 min at 55°C incubation respectively. The peptide content of both FPH increased as hydrolysis time increases. FPH showed higher crude protein content and lower fat, moisture and ash content compared to raw catfish. The major amino acids of both hydrolysates were Glu, Lys and Asp. Content of essential amino acids of Alcalase and Papain hydrolysates were 44.05% and 43.31% respectively.

  16. Energy costs of catfish space use as determined by biotelemetry.

    Directory of Open Access Journals (Sweden)

    Ondřej Slavík

    Full Text Available Animals use dispersed resources within their home range (HR during regular day-to-day activities. The high-quality area intensively used by an individual, where critical resources are concentrated, has been designated as the core area (CA. This study aimed to describe how animals utilize energy in the HR and CA assuming that changes would occur according to the size of the used areas. We observed energetic costs of space use in the largest European freshwater predator catfish, Silurus glanis, using physiological sensors. Catfish consumed significantly more energy within the CA compared to the rest of the HR area. In addition, energetic costs of space use within a large area were lower. These results generally indicate that utilization of larger areas is related to less demanding activities, such as patrolling and searching for new resources and mates. In contrast, fish occurrence in small areas appears to be related to energetically demanding use of spatially limited resources.

  17. Bioaccumulation of P-32 in bluegill and catfish

    International Nuclear Information System (INIS)

    Bluegill and catfish were fed P-32 at a constant feeding rate per body weight to determine the bioaccummulation factor (BF/sub r/) for P-32 in muscle relative to water. The fish were maintained in flow-through tanks at two feeding levels. The bluegill accumulated P-32 for 51 days, followed by depuration for 28 days. The catfish study had to be teminated after 11 days. Fish were analyzed in triplicte for P-32 and phosphorus at intervals of 1 to 8 days. Additional aquaria experiments were performed to determine the effects of water temperature, feeding rate, and type of food (worms vs. pellets) on P-32 uptake, and to observe P-32 uptake from water by unfed fish (including fish with blocked esophagus). A simple calculational model was used to determine the phosphorus turnover constant from the specific activity in tissue relative to food. This ratio at steady state approaches the BF/sub r/BF ratio (where BF is the phosphorus bioaccumulation factor) if P-32 transfers rapidly from water to food. The bluegill showed a weight gain of 0.2 %/d, a phosphorous turnover constant in muscle of 0.43 %/d, and a BF/sub r//BF ratio of 0.081 at the higher feeding rate, and 0.05 %/d, 0.34 %/d, and 0.064 at the lower feeding rate. Hence, respective P-32 BF/sub r/ values are 6000 and 4000 at a phosphorus BF of 70,000. The BF/sub r/ values for catfish were approximately twice as high. The aquarium experiments suggest that the higher factors are due to a much higher phosphorus intake, higher water temperature, higher retention from pellets than from worms, and possible higher retention by catfish than bluegill under the same conditions. 36 references, 15 figures, 22 tables

  18. DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment

    OpenAIRE

    Wong, Li Lian; Peatman, Eric; Lu, Jianguo; Kucuktas, Huseyin; He, Shunping; Zhou, Chuanjiang; Na-Nakorn, Uthairat; Liu, Zhanjiang

    2011-01-01

    As the global market for fisheries and aquaculture products expands, mislabeling of these products has become a growing concern in the food safety arena. Molecular species identification techniques hold the potential for rapid, accurate assessment of proper labeling. Here we developed and evaluated DNA barcodes for use in differentiating United States domestic and imported catfish species. First, we sequenced 651 base-pair barcodes from the cytochrome oxidase I (COI) gene from individuals of ...

  19. The Neotropical whale catfishes (Siluriformes: Cetopsidae: Cetopsinae), a revisionary study

    OpenAIRE

    Richard P. Vari; Carl J. Ferraris Jr.; Mário C. C. de Pinna

    2005-01-01

    The catfishes of the subfamily Cetopsinae of the Neotropical family Cetopsidae are revised. Four genera, Cetopsidium new genus, Cetopsis, Denticetopsis, and Paracetopsis Bleeker are recognized as valid. Bathycetopsis, Hemicetopsis, and Pseudocetopsis are considered synonyms of Cetopsis and Paracetopsis Eigenmann & Bean and Cetopsogiton synonyms of Paracetopsis. Thirty-seven species are recognized in the Cetopsinae. Cetopsidium includes six species: C. ferreirai, new species, rio Trombetas; C....

  20. Basic steps in the production of the African catfish seed

    OpenAIRE

    Ondhoro, C.C.; Mwanja, T.M.

    2014-01-01

    A typical production cycle for African catfish farming begins with a selection of fingerlings or juvenile fish of good quality for brood stock development. Fish are selected from a family or grow out stock basing on records of the origin,age, strain and performance history of the parents or from the wild in this brochure, we explain the basic steps and requirements a farmer needs in order to achieve good results in the hatchery.

  1. A First Generation Bac-Based Physical Map of the Channel Catfish Genome

    Science.gov (United States)

    Background: Channel catfish, Ictalurus punctatus, is the leading species in North American aquaculture. Genetic improvement of catfish is performed through selective breeding, and genomic tools will help improve selection efficiency. A physical map is needed to integrate the genetic map with the kar...

  2. Stomach Contents of the Catfish (Clarias gariepinus Burchell, 1822) in the River Asi (Turkey)

    OpenAIRE

    YALÇIN, Şükran

    2001-01-01

    Natural food items of catfish (Clarias gariepinus Burchell, 1822) were investigated in the River Asi from September 1996 to October 1998. Analyses of stomach contents showed that the catfish feed basically on Arthropoda, mostly Diptera larvae, and on plant materials to a considerable extent. A successful filter feeding was observed, especially during the summer period.

  3. A trap panel for in-pond raceways to capture escaped catfish

    Science.gov (United States)

    The first commercial-scale in-pond raceway system (IPRS) used to produce catfish in west Alabama was constructed from funds by a joint effort involving the Alabama Catfish Producers Association, Dean Wilson Farms, and Auburn University. The goal of this project was to improve profitability of catfis...

  4. Effects of Endosulfan on Predator–Prey Interactions Between Catfish and Schistosoma Host Snails

    NARCIS (Netherlands)

    Monde, Concillia; Syampungani, Stephen; Brink, van den Paul J.

    2016-01-01

    The effect of the pesticide endosulfan on predator–prey interactions between catfish and Schistosoma host snails was assessed in static tank experiments. Hybrid catfish (Clarias gariepinus × C. ngamensis) and Bulinus globosus were subjected to various endosulfan concentrations including an untrea

  5. Remediation of uranium-contaminated groundwater by sorption onto hydoxyapatite derived from catfish bones

    Science.gov (United States)

    Hydroxyapatite was prepared from catfish bones, called catfish hydroxyapatite (CFHA), by mechanical and chemical treatment methods and was characterized by x-ray diffraction (X-RD) and scanning electron microscope (SEM) techniques to confirm the presence of hydroxyapatite. The ability of CFHA to rem...

  6. Draft genome sequences of four virulent aeromonas hydrophila strains from catfish aquaculture

    Science.gov (United States)

    Since 2009, a clonal group of virulent Aeromonas hydrophila (VAh) strains has been causing severe disease in the catfish aquaculture industry in the Southeastern United States. Here, we report draft genomes of four A. hydrophila isolates from catfish aquaculture that represent this clonal group....

  7. Zebrafish (Danio rerio) bioassay for visceral toxicosis of catfish and botulinum neurotoxin serotype E

    Science.gov (United States)

    Visceral toxicosis of catfish (VTC), a sporadic disease of cultured channel catfish (Ictalurus punctatus) often with high mortality, is caused by botulinum neurotoxin serotype E (BoNT/E). Presumptive diagnosis of VTC is based on characteristic clinical signs and lesions, and the production of these ...

  8. Biofloc production technology promising in temperate zones – Channel catfish perform during winter months

    Science.gov (United States)

    A study was conducted to assess the feasibility to grow Channel catfish (Ictalurus punctatus) in an outdoor biofloc system during winter in a temperate zone. High biomasses of market-size channel catfish were successfully maintained through the winter with high survival and in good condition in both...

  9. Plasma polar lipid profiles of channel catfish with different growth rates

    Science.gov (United States)

    Increased growth in channel catfish is an economically important trait and has been used as a criterion for the selection and development of brood fish. Selection of channel catfish toward increased growth usually results in the accumulation of large amounts of fats in their abdomen rather than incr...

  10. Performance evaluation of intensive, pond-based culture systems for catfish production in Mississippi: year one

    Science.gov (United States)

    Catfish farming is the leading form of aquaculture in the U.S. and has a significant economic impact in the southern states. Increased feed and energy costs, and competition from foreign imports have recently caused catfish farmers to intensify production as a means of improving efficiency and profi...

  11. Draft Genome Sequences of Four Virulent Aeromonas hydrophila Strains from Catfish Aquaculture

    Science.gov (United States)

    Tekedar, Hasan C.; Kumru, Salih; Karsi, Attila; Waldbieser, Geoffrey C.; Sonstegard, Tad; Schroeder, Steven G.; Liles, Mark R.; Griffin, Matt J.

    2016-01-01

    Since 2009, a clonal group of virulent Aeromonas hydrophila strains has been causing severe disease in the catfish aquaculture industry in the southeastern United States. Here, we report draft genomes of four A. hydrophila isolates from catfish aquaculture that represent this clonal group. PMID:27540076

  12. Effects of metomindate hydrochloride and tricaine methanesulfonate on the short term cortisol response in channel catfish

    Science.gov (United States)

    The effects of metomidate hydrochloride and tricaine methanesulfonate (MS-222) on cortisol stress response of channel catfish, Ictalurus punctatus, were examined during 10 minutes of sedation. Channel catfish were assigned to three treatments: 1. Metomidate hydrochloride (12.5 mg/L), 2. MS-222 (100...

  13. Response of channel x blue hybrid catfish to chronic diurnal hypoxia

    Science.gov (United States)

    Performance traits and metabolic responses of the channel x blue hybrid catfish (Ictalurus punctatus female x I. furcatus male) in response to chronic diurnal hypoxia were evaluated in this 197-d study. Sixteen 0.1-ha earthen ponds were stocked with 15,169 hybrid catfish/ha (47 g/fish) and managed t...

  14. Evaluation of Catfish Skin Hydrolysates as a Glazing Material for Air-Blast Frozen Shrimp

    Science.gov (United States)

    Catfish is one of the most widely consumed seafood in the United States. A by-product of this consumption is a large quantity of catfish skin (CS), approximately 8,200 metric tons in 2014. Enzymatic hydrolysis is used to produce protein hydrolysates from the skin. These hydrolysates have considerabl...

  15. Molecular Cloning and Sequencing of Hemoglobin-Beta Gene of Channel Catfish, Ictalurus Punctatus Rafinesque

    Science.gov (United States)

    : Hemoglobin-y gene of channel catfish , lctalurus punctatus, was cloned and sequenced . Total RNA from head kidneys was isolated, reverse transcribed and amplified . The sequence of the channel catfish hemoglobin-y gene consists of 600 nucleotides . Analysis of the nucleotide sequence reveals one o...

  16. Molecular Cloning, Expression and Genome Organization of Channel Catfish (Ictalurus punctatus) Matrix Metalloproteinase-9

    Science.gov (United States)

    In the course of studying pathogenesis of enteric septicemia of catfish, we noted that channel catfish matrix metalloproteinase-9 (MMP-9) gene was up-regulated after Edwardsiella ictaluri infection. In this study, we cloned, sequenced using the RACE (rapid amplification of cDNA ends) method and cha...

  17. Assembly of 500,000 inter-specific catfish expressed sequence tags and large scale gene-associated marker development for whole genome association studies

    OpenAIRE

    Wang, Shaolin

    2010-01-01

    Background-Through the Community Sequencing Program, a catfish EST sequencing project was carried out through a collaboration between the catfish research community and the Department of Energy's Joint Genome Institute. Prior to this project, only a limited EST resource from catfish was available for the purpose of SNP identification. Results-A total of 438,321 quality ESTs were generated from 8 channel catfish (Ictalurus punctatus) and 4 blue catfish (Ictalurus furcatus) libraries, bringing ...

  18. Assembly of 500,000 inter-specific catfish expressed sequence tags and large scale gene-associated marker development for whole genome association studies

    OpenAIRE

    Wang, Shaolin; Peatman, Eric; Abernathy, Jason; Waldbieser, Geoff; Lindquist, Erika; Richardson, Paul; Lucas, Susan; Wang, Mei; Li, Ping; Thimmapuram, Jyothi; Liu, Lei; Vullaganti, Deepika; Kucuktas, Huseyin; Murdock, Christopher; Small, Brian C

    2010-01-01

    Background Through the Community Sequencing Program, a catfish EST sequencing project was carried out through a collaboration between the catfish research community and the Department of Energy's Joint Genome Institute. Prior to this project, only a limited EST resource from catfish was available for the purpose of SNP identification. Results A total of 438,321 quality ESTs were generated from 8 channel catfish (Ictalurus punctatus) and 4 blue catfish (Ictalurus furcatus) libraries, bringing ...

  19. The use of hematology method and blood endoparasite observation for determining catfish (Clarias gariepinus) health in fishery Mangkubumen, Boyolali

    OpenAIRE

    AGUNG BUDIHARJO; NOOR SOESANTI HANDAJANI; INTAN ESTETIKA ALAMANDA

    2007-01-01

    This research aims to find out the health condition of catfish (Clarias gariepinus) based on hematology and to find out the blood endoparasite type of catfish (Clarias gariepinus) bred in Mangkubumen Boyolali. The sample collection of catfish in this research was conducted in Mangkubumen, Boyolali. The catfish becoming the sample had following criteria: 3 month old, 100-150 gr weight, and 20-25 cm long. The sample of catfish was taked from 5 pools, it was taken 10 fishes from each pool. The ...

  20. Comparative analysis of catfish BAC end sequences with the zebrafish genome

    Directory of Open Access Journals (Sweden)

    Abernathy Jason

    2009-12-01

    Full Text Available Abstract Background Comparative mapping is a powerful tool to transfer genomic information from sequenced genomes to closely related species for which whole genome sequence data are not yet available. However, such an approach is still very limited in catfish, the most important aquaculture species in the United States. This project was initiated to generate additional BAC end sequences and demonstrate their applications in comparative mapping in catfish. Results We reported the generation of 43,000 BAC end sequences and their applications for comparative genome analysis in catfish. Using these and the additional 20,000 existing BAC end sequences as a resource along with linkage mapping and existing physical map, conserved syntenic regions were identified between the catfish and zebrafish genomes. A total of 10,943 catfish BAC end sequences (17.3% had significant BLAST hits to the zebrafish genome (cutoff value ≤ e-5, of which 3,221 were unique gene hits, providing a platform for comparative mapping based on locations of these genes in catfish and zebrafish. Genetic linkage mapping of microsatellites associated with contigs allowed identification of large conserved genomic segments and construction of super scaffolds. Conclusion BAC end sequences and their associated polymorphic markers are great resources for comparative genome analysis in catfish. Highly conserved chromosomal regions were identified to exist between catfish and zebrafish. However, it appears that the level of conservation at local genomic regions are high while a high level of chromosomal shuffling and rearrangements exist between catfish and zebrafish genomes. Orthologous regions established through comparative analysis should facilitate both structural and functional genome analysis in catfish.

  1. Induction of lauric acid omega-hydroxylation by peroxisomal proliferators in bluegill and catfish

    Energy Technology Data Exchange (ETDEWEB)

    Haasch, M.L. [Univ. of Maryland, Solomons, MD (United States); Henderson, M.C.; Buhler, D.R. [Oregon State Univ. Corvallis, OR (United States)

    1995-12-31

    Peroxisome proliferating agents (PPAs) are a structurally diverse group of chemicals that include environmental chemical contaminants such as certain chlorinated herbicides, solvents and plasticizers. PPAs have previously been shown to induce anti-trout laruci acid hydroxylase immunoreactive proteins in bluegill and catfish. In this investigation, induction of lauric acid hydroxylase activity and immunoreactive proteins was confirmed, and the mass spectral analysis of specific hydroxylation products was performed in order to identify possible species-specific differences in fatty acid metabolism. Male bluegill (Lepomis macrochirus) and channel catfish (Ictalurus punctatus) were administered clofibrate or ciprofibrate 48 hr prior to hepatic or trunk kidney (catfish only) microsome preparation. While no significant differences were observed in male catfish, male bluegill had significant decreases in hematocrit and plasma protein indicating hemodilution due to possible gill or kidney damage. Both bluegill and catfish exhibited induction of hepatic and kidney (catfish only) anti-trout lauric acid hydroxylase immunoreactive proteins. In general, total metabolism of lauric acid was greater, and higher levels of wP2, wP3, and wP4 products were produced in control catfish than in juvenile male trout. In male bluegill, lauric acid hydroxylation products wP, wP4 and wP5 were significantly induced by clofibrate treatment. Taken together the above data indicate that peroxisome proliferation may be an important consideration for responsive species exposed to PPAs by environmental chemical contamination.

  2. Avaliação do efeito tóxico de sulfato de alumínio e sulfato de cobre em bioensaio de contaminação subcrônica via trófica no bioindicador Rhamdia quelen (Siluriforme)

    OpenAIRE

    Costa, Paula Moiana da, 1978-

    2012-01-01

    Resumo: O cobre é um elemento essencial para a atividade de diversas enzimas biológicas, mas em doses altas é considerado altamente tóxico às algas, fungos, sementes de plantas e invertebrados, e moderadamente tóxico aos mamíferos. Relata-se, especialmente, sua toxicidade a invertebrados marinhos e de água doce. O alumínio está presente em pequenas quantidades nos organismos vivos, mas é abundante no ambiente. Entretanto, estudos mostram que o aumento do alumínio dissolvido na água causa dano...

  3. Disposition of 1-naphthol in the channel catfish

    International Nuclear Information System (INIS)

    Limited data exists on the disposition of xenobiotics in the channel catfish. The present study examines 1-naphthol as a model compound for phase 2 metabolism and dose dependent kinetics in this species. 14C-Naphthol was administered orally or intravascularly at 1, 5 or 25 mg/kg. Plasma levels of naphthol after intravascular dosing were fitted to a three compartment pharmacokinetic model. There was a dose related change in several pharmacokinetic parameters but little evidence of metabolic saturation. Approximately 60% of the oral dose was renally excreted in 24 hr as glucuronide and sulfate conjugates. The bile also contained the glucuronide and an unidentified polar metabolite, but little or no sulfate. The low bioavailability (38%) of naphthol was probably a result of first pass metabolism. The edible flesh had the lowest levels of residual compound while the liver and trunk kidney had the highest. Approximately 1% of the dose remained in the tissues at 24 hr. This study demonstrates that 1-naphthol is readily conjugated and rapidly eliminated in catfish. The dose dependency of kinetic values may have resulted from saturable tissue binding

  4. Improved method for sectioning pectoral spines of catfish for age determination

    Science.gov (United States)

    Blouin, Marc A.; Hall, Glenda R.

    1990-01-01

    A modified low-speed saw provided fast and precise sectioning of catfish pectoral spines for use in aging studies. In one hour, 10–15 spines can be sectioned, the sections mounted, and the annuli counted.

  5. Radioactivity studies on farm raised and wild catfish produced in Mississippi, USA

    International Nuclear Information System (INIS)

    To understand the contribution of radiation dose to human from consumption of catfish, a comparative radiological assessment study was performed on farm and river raised fish. Gamma spectroscopic analyses of catfish indicated the presence of isotopes of 40K, 226Ra, and 228Ra with activity values of 99.4 ± 14.5, 0.41 ± 0.05, and 0.89 ± 0.08 Bq kg-1 respectively in farm catfish and 101 ± 15.7, 0.47 ± 0.05, and 0.97 ± 0.050 Bq kg-1 respectively in river catfish. The measured activities in two sets of samples were statistically compared using a two-tailed t test at 95 % confidence interval and results indicated that activities of 226Ra and 228Ra were significantly different. (author)

  6. Short-term feed deprivation alters immune status of surface mucosa in channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    Short-term feed deprivation (or fasting) is a common occurrence in aquacultured fish species whether due to season, production strategies, or disease. In channel catfish (Ictalurus punctatus) fasting impacts susceptibility to several bacterial pathogens including Flavobacterium columnare, the causat...

  7. The fry patterns in the South-American catfish genus Corydoras (Pisces, Siluriformes, Callichthyidae)

    OpenAIRE

    Fuller, Ian

    1999-01-01

    The fry pattern of aquarium-bred species of the South-American catfish genus Corydoras, contribute to discriminate between species of the so-called ‘acutus’-group, showing a greyish adult colour pattern.

  8. Effect of Microwave Pretreatment on Extraction Yield and Quality of Catfish Oil in Northern Thailand

    Directory of Open Access Journals (Sweden)

    Chimsook Thitiphan

    2015-01-01

    Full Text Available The effect of microwave pretreatment of catfish processing waste on oil recovery and quality was investigated. Fish oil was extracted using the enzymatic hydrolysis using alcalase enzyme after the microwave pretreatment. The effect of microwave power and microwave pretreatment times was evaluated. The results revealed that a highest yield of 9.25% when catfish waste was treated at 110 W for 60 s. This condition was found as the most appropriate condition of microwave pretreatment since it decreased the extraction time from 150 min to up to 30 min. Analysis of oil quality indices (e.g. acid value, p-anisidine value, peroxide value extracted by microwave pretreatment and non-pretreatment indicated that catfish oil from both processes has comparatively similar fatty acids composition. In addition, catfish oil was extracted by microwave pretreatment and enzymatic hydrolysis had lower lipid oxidation compared to several standards.

  9. Chronic pathology and longevity of Drepanocephalus spathans infections in juvenile channel catfish

    Science.gov (United States)

    Drepanocephalus spathans (Digenea: Echinostomatidae) is a common parasite of the double-crested cormorant (Phalacrocorax auritus). The cercariae of D. spathans have been shown infective to juvenile channel catfish (Ictalurus punctatus). The developing metacercariae concentrate in the cranial regio...

  10. An Asian Origin of Virulent Aeromonas hydrophila Responsible for Disease Epidemics in United States-Farmed Catfish

    OpenAIRE

    Hossain, Mohammad J.; Sun, Dawei; McGarey, Donald J.; Wrenn, Shannon; Alexander, Laura M.; Martino, Maria Elena; Xing, Ye; Terhune, Jeffery S.; Liles, Mark R.

    2014-01-01

    ABSTRACT Since 2009, catfish farming in the southeastern United States has been severely impacted by a highly virulent and clonal population of Aeromonas hydrophila causing motile Aeromonas septicemia (MAS) in catfish. The possible origin of this newly emerged highly virulent A. hydrophila strain is unknown. In this study, we show using whole-genome sequencing and comparative genomics that A. hydrophila isolates from diseased grass carp in China and catfish in the United States have highly si...

  11. Assessment, Development and Implementation of Training Materials for Food Defense/Safety, Biosecurity, and Traceability within the Catfish Industry

    OpenAIRE

    Hood, Anna F.; Williams, J. Byron; Crist, Courtney

    2012-01-01

    The objective of this research and subsequent training sessions was to assess food defense in the catfish industry, promote awareness and educate catfish farmers and processors on the principles of food defense, biosecurity and traceability of agricultural commodities and ingredients used in human food production. In the catfish processing chain, preliminary investigations have indicated that there are areas throughout production, processing and distribution that are susceptible to potential ...

  12. The impacts of non-tariff barriers on the export price of Vietnamese catfish

    OpenAIRE

    Khuu, Thi Phuong Dong

    2012-01-01

    The aims of this study to identify the impacts of non-tariff barriers (NTBs) on Vietnamese catfish export price in the international market. The time-series monthly data from 1999 to 2011 was used in our paper. Base on the demand and supply equilibrium theory, we constructed the function of Vietnamese catfish export price. The unit toot test results showed that our data was stationary at differenced level. Therefore, the econometric models, such as first-differenced model, Error correction mo...

  13. Comparative analysis of catfish BAC end sequences with the zebrafish genome

    OpenAIRE

    Abernathy Jason; Xu Peng; Somridhivej Benjaporn; Ninwichian Parichart; Wang Shaolin; Jiang Yanliang; Liu Hong; Kucuktas Huseyin; Liu Zhanjiang

    2009-01-01

    Abstract Background Comparative mapping is a powerful tool to transfer genomic information from sequenced genomes to closely related species for which whole genome sequence data are not yet available. However, such an approach is still very limited in catfish, the most important aquaculture species in the United States. This project was initiated to generate additional BAC end sequences and demonstrate their applications in comparative mapping in catfish. Results We reported the generation of...

  14. College Students' Opinions of U.S. Farm-Raised Catfish

    OpenAIRE

    Hanson, Terrill R.; Rose, Patrick

    2011-01-01

    A survey focusing on factors related to consumption of fish and seafood, including catfish, and targeted at college-aged students was developed and administered in conjunction with Auburn University's Earth Day dining promotion. Six hundred forty completed surveys were obtained over two days in April, 2012. Findings suggest that students had an overall positive opinion toward catfish, citing enjoyment of flavor (44 percent), better texture (38 percent), less expensive (43 percent), and greate...

  15. Whole genome comparative analysis of channel catfish (Ictalurus punctatus) with four model fish species

    OpenAIRE

    Jiang, Yanliang; Gao, Xiaoyu; Liu, Shikai; Zhang, Yu; Liu, Hong; Sun, Fanyue; Bao, Lisui; Waldbieser, Geoff; Liu, Zhanjiang

    2013-01-01

    Background Comparative mapping is a powerful tool to study evolution of genomes. It allows transfer of genome information from the well-studied model species to non-model species. Catfish is an economically important aquaculture species in United States. A large amount of genome resources have been developed from catfish including genetic linkage maps, physical maps, BAC end sequences (BES), integrated linkage and physical maps using BES-derived markers, physical map contig-specific sequences...

  16. Comparative analysis of catfish BAC end sequences with the zebrafish genome

    OpenAIRE

    Liu, Hong; Jiang, Yanliang; Wang, Shaolin; Ninwichian, Parichart; Somridhivej, Benjaporn; Xu, Peng(Academy of Mathematics and Systems Science, Chinese Academy of Sciences, 100190, Beijing, China); Abernathy, Jason; Kucuktas, Huseyin; Liu, Zhanjiang

    2009-01-01

    Background Comparative mapping is a powerful tool to transfer genomic information from sequenced genomes to closely related species for which whole genome sequence data are not yet available. However, such an approach is still very limited in catfish, the most important aquaculture species in the United States. This project was initiated to generate additional BAC end sequences and demonstrate their applications in comparative mapping in catfish. Results We reported the generation of 43,000 B...

  17. Distribution of catfishes in wetlands of two flood plain districts in Tamil Nadu, India

    OpenAIRE

    B. Rajagopal; Davidar, P.

    2013-01-01

    A study was conducted on the distribution of catfishes in selected wetlands in Kancheepuram and Kanyakumari districts of Tamil Nadu, southern India. Different types of wetlands such as tanks, pools, lakes, open wells and estuaries were selected for the study based on their different environmental set up. Fishes were collected with the help of fishermen using cast and seine nets. Twelve species of catfishes from five families (Ariidae, Bagridae, Heteropneustidae, Schilbeidae and Siluridae) wer...

  18. Delivering the Goods: Lessons Learned from Direct Delivery of Kentucky Catfish

    OpenAIRE

    Berney, Gerald; Tropp, Debra; Clifton, Kimberly; McKenna, Larissa

    2007-01-01

    USDA initiated a pilot study in cooperation with KDA and PAAC that examined the marketing, transportation, and delivery strategies of the catfish processing cooperative. The information gathered should prove useful to many groups involved in this type of enterprise. The study had several emphases: • Describing the marketing channels, customers, and products used by the cooperative in selling catfish. • Describing the procedures and equipment used in PAAC’s direct delivery operation. • Examini...

  19. Relative condition factor and food and feeding habits of two ariid catfish from Mumbai waters

    OpenAIRE

    Deo, A.D.; Venkateshvaran, K.; Devaraj, M.

    2010-01-01

    Condition factor based on length-weight relationship and food and feeding habits of Arius dussumieri (Black lip sea catfish) and Osteogeneiosus militaris (Soldier catfish) from the Mumbai coast have been studied. Relative Condition Factor showed variations on a monthly basis, and appear to be influenced by feeding and breeding activities. Both the species studied are carnivorous bottom feeders, with crustaceans followed by other smaller fishes forming the major food item in the gut contents.

  20. On-farm feed resources for catfish (Clarias gariepinus) production in Laos

    OpenAIRE

    Phonekhampheng, Oudom

    2008-01-01

    The aim of the thesis was to collected information African catfish (Clarias gariepinus) production systems, and the chemical composition and nutritive value of potentially available feedstuffs. Moreover, the apparent digestibility (AD) of nutrient and amino acids (AA) in raw, sun-dried and ensiled Golden Apple snails (GAS) was evaluated, as well as the potential of GAS to replace fish meal in the diet for growing African catfish fingerlings. The farmers used traditional feedstuffs such as mai...

  1. Karyotype Analysis of the New Catfish Mystus ngasep (Siluriformes: Bagridae) from Manipur, India

    OpenAIRE

    Singh, Sukham Sanjabihari; Singh, Chingakham Brajakishor; WAIKHOM, Gusheinzed

    2013-01-01

    Karyotypic and cytogenetic characteristics of catfish Mystus ngasep, a new species of bagrid catfish described from the Northeast India, Manipur was studied for the first time by examining 200 metaphase spreads chromosome from the kidney cells of 25 healthy specimens. The diploid chromosome number of this species was 2n=56 and the total fundamental arm number was determined as NF=90. The karyotype consisted of 12 Metacentric (m), 22 Submetacentric (Sm), 8 Subtelocentric (St) and 14 Teloce...

  2. The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.

    Science.gov (United States)

    Liu, Zhanjiang; Liu, Shikai; Yao, Jun; Bao, Lisui; Zhang, Jiaren; Li, Yun; Jiang, Chen; Sun, Luyang; Wang, Ruijia; Zhang, Yu; Zhou, Tao; Zeng, Qifan; Fu, Qiang; Gao, Sen; Li, Ning; Koren, Sergey; Jiang, Yanliang; Zimin, Aleksey; Xu, Peng; Phillippy, Adam M; Geng, Xin; Song, Lin; Sun, Fanyue; Li, Chao; Wang, Xiaozhu; Chen, Ailu; Jin, Yulin; Yuan, Zihao; Yang, Yujia; Tan, Suxu; Peatman, Eric; Lu, Jianguo; Qin, Zhenkui; Dunham, Rex; Li, Zhaoxia; Sonstegard, Tad; Feng, Jianbin; Danzmann, Roy G; Schroeder, Steven; Scheffler, Brian; Duke, Mary V; Ballard, Linda; Kucuktas, Huseyin; Kaltenboeck, Ludmilla; Liu, Haixia; Armbruster, Jonathan; Xie, Yangjie; Kirby, Mona L; Tian, Yi; Flanagan, Mary Elizabeth; Mu, Weijie; Waldbieser, Geoffrey C

    2016-01-01

    Catfish represent 12% of teleost or 6.3% of all vertebrate species, and are of enormous economic value. Here we report a high-quality reference genome sequence of channel catfish (Ictalurus punctatus), the major aquaculture species in the US. The reference genome sequence was validated by genetic mapping of 54,000 SNPs, and annotated with 26,661 predicted protein-coding genes. Through comparative analysis of genomes and transcriptomes of scaled and scaleless fish and scale regeneration experiments, we address the genomic basis for the most striking physical characteristic of catfish, the evolutionary loss of scales and provide evidence that lack of secretory calcium-binding phosphoproteins accounts for the evolutionary loss of scales in catfish. The channel catfish reference genome sequence, along with two additional genome sequences and transcriptomes of scaled catfishes, provide crucial resources for evolutionary and biological studies. This work also demonstrates the power of comparative subtraction of candidate genes for traits of structural significance. PMID:27249958

  3. Effect of stocking biomass on solids, phytoplankton communities, common off-flavors, and production parameters in a channel catfish biofloc technology production system

    Science.gov (United States)

    The effect of initial channel catfish (Ictalurus punctatus, Rafinesque, 1818) fingerling biomass (1.4, 1.8, or 2.3 kg m-3) on phytoplankton communities, common off-flavors, and stocker catfish production parameters was evaluated in biofloc technology production tanks. Stocker catfish size (145.5 – 1...

  4. Assembly of 500,000 Inter-Specific Catfish Expressed Sequence Tags and Large Scale Gene-Associated Marker Development for Whole Genome Association Studies

    Science.gov (United States)

    Through the Community Sequencing Program, a catfish EST sequencing project was carried out through a collaboration between the catfish research community and the Department of Energy's Joint Genome Institute. Prior to this project, only a limited EST resource from catfish was available for the purpo...

  5. Physiological responses of Chinese longsnout catfish to water temperature

    Science.gov (United States)

    Han, Dong; Xie, Shouqi; Zhu, Xiaoming; Yang, Yunxia

    2011-05-01

    We evaluated the effect of water temperature on the growth and physiology of the Chinese longsnout catfish ( Leiocassis longirostris Günther). The fish were reared at four temperatures (20, 25, 30, and 35°C) and sampled on days 7, 20, and 30. We measured plasma levels of insulin, free thyroxine (FT4), free 3,5,3'-triiodothyronine (FT3), lysozyme and leukocyte phagocytic activity. The optimum water temperature for growth was 27.7°C. The plasma levels of insulin and FT4 declined significantly ( P<0.05) on day 30 at temperatures above 20°C. Lysozyme activity was significantly ( P<0.05) lower at 25°C than at other temperatures. We conclude that final weight, insulin, FT4, and lysozyme were significantly affected by water temperature.

  6. Risk analysis of tractor overturns on catfish farms.

    Science.gov (United States)

    Stephens, Walter B; Ibendahl, Gregory A; Myers, Melvin L; Cole, Henry P

    2010-10-01

    Fatal and nonfatal injuries occur on catfish farms as a result of tractor overturns, but these injuries can be greatly mitigated when a tractor is equipped with a rollover protective structure (ROPS) and seatbelt. This study analyzed the proportion of tractors on catfish farms in Mississippi not equipped with ROPS and the cost of retrofitting those tractors with a ROPS and seatbelt as compared to the expected benefits gained from adding the protection. To determine if farmers have the financial incentive to retrofit older tractors, a net present value framework was used because the expected benefits occur over a number of years. The ROPS retrofit is a one-time cost that occurs immediately, and thus the present value does not need to be calculated, i.e., time period 0. According to this study adding ROPS will provide a net benefit of $22,877 in the event of an overturn. When dealing with small farms (tractor is used for approximately twice as many hours compared to an average tractor on large farms, the extra hours worked by a single tractor resulted in total benefits of retrofitting ROPS of $9.45 per year, whereas for large farms the net benefit is $4.70 (=125 acres). Given that the least expensive retrofit is $147, it would take 15.5 years of tractor use on the small farms for the expected benefits to outweigh the cost. Given the large range of costs that could occur due to an overturn, there could be a significant premium for not having ROPS protection. In other words, there likely is a smaller range of costs from an overturn with ROPS than without ROPS because ROPS reduces the more serious incidents. PMID:20954036

  7. Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil

    International Nuclear Information System (INIS)

    The objective of this study was to identify the possible involvement of the GABAergic system in the anesthetic effect of Lippia alba essential oil (EO). We propose a new animal model using silver catfish (Rhamdia quelen) exposed to an anesthetic bath to study the mechanism of action of EO. To observe the induction and potentiation of the anesthetic effect of EO, juvenile silver catfish (9.30 ± 1.85 g; 10.15 ± 0.95 cm; N = 6) were exposed to various concentrations of L. alba EO in the presence or absence of diazepam [an agonist of high-affinity binding sites for benzodiazepinic (BDZ) sites coupled to the GABAA receptor complex]. In another experiment, fish (N = 6) were initially anesthetized with the EO and then transferred to an anesthetic-free aquarium containing flumazenil (a selective antagonist of binding sites for BDZ coupled to the GABAA receptor complex) or water to assess recovery time from the anesthesia. In this case, flumazenil was used to observe the involvement of the GABA-BDZ receptor in the EO mechanism of action. The results showed that diazepam potentiates the anesthetic effect of EO at all concentrations tested. Fish exposed to diazepam and EO showed faster recovery from anesthesia when flumazenil was added to the recovery bath (12.0 ± 0.3 and 7.2 ± 0.7, respectively) than those exposed to water (9.2 ± 0.2 and 3.5 ± 0.3, respectively). In conclusion, the results demonstrated the involvement of the GABAergic system in the anesthetic effect of L. alba EO on silver catfish

  8. Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil

    Energy Technology Data Exchange (ETDEWEB)

    Heldwein, C.G.; Silva, L.L. [Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Reckziegel, P. [Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Barros, F.M.C. [Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Bürger, M.E.; Baldisserotto, B. [Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Mallmann, C.A. [Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil); Schmidt, D.; Caron, B.O. [Departamento de Ciências Agronômicas e Ambientais, Universidade Federal de Santa Maria, Campus de Frederico Westphalen, Frederico Westphalen, RS (Brazil); Heinzmann, B.M. [Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS (Brazil)

    2012-04-05

    The objective of this study was to identify the possible involvement of the GABAergic system in the anesthetic effect of Lippia alba essential oil (EO). We propose a new animal model using silver catfish (Rhamdia quelen) exposed to an anesthetic bath to study the mechanism of action of EO. To observe the induction and potentiation of the anesthetic effect of EO, juvenile silver catfish (9.30 ± 1.85 g; 10.15 ± 0.95 cm; N = 6) were exposed to various concentrations of L. alba EO in the presence or absence of diazepam [an agonist of high-affinity binding sites for benzodiazepinic (BDZ) sites coupled to the GABA{sub A} receptor complex]. In another experiment, fish (N = 6) were initially anesthetized with the EO and then transferred to an anesthetic-free aquarium containing flumazenil (a selective antagonist of binding sites for BDZ coupled to the GABA{sub A} receptor complex) or water to assess recovery time from the anesthesia. In this case, flumazenil was used to observe the involvement of the GABA-BDZ receptor in the EO mechanism of action. The results showed that diazepam potentiates the anesthetic effect of EO at all concentrations tested. Fish exposed to diazepam and EO showed faster recovery from anesthesia when flumazenil was added to the recovery bath (12.0 ± 0.3 and 7.2 ± 0.7, respectively) than those exposed to water (9.2 ± 0.2 and 3.5 ± 0.3, respectively). In conclusion, the results demonstrated the involvement of the GABAergic system in the anesthetic effect of L. alba EO on silver catfish.

  9. Copper use and accumulation in catfish culture in the Mekong Delta, Vietnam.

    Science.gov (United States)

    Marcussen, Helle; Løjmand, Helle; Dalsgaard, Anders; Hai, Dao M; Holm, Peter E

    2014-01-01

    Aquaculture of Pangasius hypophthalmus (striped catfish) in Vietnam reached 1.1 million tonnes in 2011 and catfish fillets are exported worldwide. The intensive cultures of catfish mainly in earth ponds have made it necessary to apply CuSO4 and other chemicals to control external parasites and other pathogens. However, accumulation of Cu in aquaculture ponds may pose a hazard to growth of fish or to the aquatic environment. The aim of this study was to determine accumulation of Cu in sediment, water and fish in a catfish pond with a history of repeated treatment with CuSO4 in the Mekong Delta, Vietnam. Copper concentrations in pond sediment were in the interval 21.3-45.7 mg kg(-1) dw and did not exceed the Vietnamese values for soil to be used for agricultural production (70 mg kg(-1) dw.). During three samplings the total mean concentration of Cu in pond water (4 μg L(-1)) did not exceed the LC50-value (70 μg L(-1)) for catfish and the mean dissolved concentration of Cu (0.986 μg L(-1)) did not seem to constitute a risk for the stability of the aquatic ecosystem. No significant variation in Cu concentrations between sampling sites in the pond and depth of sediment profile were determined. The accumulation of Cu in catfish was highest in the liver compared to the skin, gills and muscle tissue. With the current practice of removing pond sludge three to four times during a production cycle little if any Cu seems to accumulate in catfish ponds despite repeated anti-parasite treatments with CuSO4. Further studies are needed to assess the eco-toxicity and impact on agricultural production when pond sediment is discharged into aquatic recipients and used as soil fertilizer.

  10. The Holocene occurrence of the European catfish (Silurus glanis) in Belgium: the archaeozoological evidence

    OpenAIRE

    Van Neer, W.; Ervynck, A.

    2009-01-01

    An overview is given of the skeletal remains of the European catfish Silurus glanis found thus far in Belgian archaeological sites. These finds demonstrate that the species is autochthonous and allow documenting its occurrence and disappearance during the Holocene in the Scheldt and Meuse basins. Possible causes for the local extinction of this catfish are discussed.

  11. Ribosomal protein genes are highly enriched among genes with allele-specific expression in the interspecific F1 hybrid catfish.

    Science.gov (United States)

    Chen, Ailu; Wang, Ruijia; Liu, Shikai; Peatman, Eric; Sun, Luyang; Bao, Lisui; Jiang, Chen; Li, Chao; Li, Yun; Zeng, Qifan; Liu, Zhanjiang

    2016-06-01

    Interspecific hybrids provide a rich source for the analysis of allele-specific expression (ASE). In this work, we analyzed ASE in F1 hybrid catfish using RNA-Seq datasets. While the vast majority of genes were expressed with both alleles, 7-8 % SNPs exhibited significant differences in allele ratios of expression. Of the 66,251 and 177,841 SNPs identified from the datasets of the liver and gill, 5420 (8.2 %) and 13,390 (7.5 %) SNPs were identified as significant ASE-SNPs, respectively. With these SNPs, a total of 1519 and 3075 ASE-genes were identified. Gene Ontology analysis revealed that genes encoding cytoplasmic ribosomal proteins (RP) were highly enriched among ASE genes. Parent-of-origin was determined for 27 and 30 ASE RP genes in the liver and gill, respectively. The results indicated that genes from both channel catfish and blue catfish were involved in ASE. However, each RP gene appeared to be almost exclusively expressed from only one parent, indicating that ribosomes in the hybrid catfish were in the "hybrid" form. Overall representation of RP transcripts among the transcriptome appeared lower in the F1 hybrid catfish than in channel catfish or blue catfish, suggesting that the "hybrid" ribosomes may work more efficiently for translation in the F1 hybrid catfish. PMID:26747053

  12. Dioxin congener patterns in commercial catfish from the United States and the indication of mineral clays as the potential source

    Science.gov (United States)

    Since 1991 the U.S. Department of Agriculture (USDA) has conducted annual surveys of pesticide residues in foods under the Agricultural Marketing Service’s Pesticide Data Program (PDP). To assess chemical residues in domestically marketed catfish products, 1479 catfish samples were collected during ...

  13. Molecular and morphological characterization of myxozoan actinospore types from a commercial catfish pond in the Mississippi Delta

    Science.gov (United States)

    The actinospore diversity of infected Dero digitata was surveyed (May, 2011) from a channel catfish production pond in the Mississippi Delta region for the elucidation of unknown myxozoan life cycles. Only two myxozoan life cycles have been molecularly confirmed in channel catfish (Ictalurus puncta...

  14. Feeding Lactobacillus spp. and Bacillus spp. Does Not Improve Growth or Survival of Channel Catfish Experimentally Challenged with Edwardsiella ictaluri

    Science.gov (United States)

    A major problem in the channel catfish industry has been high disease loss to enteric septicemia of catfish, caused by the bacterium Edwardsiella ictaluri. Feeding probiotics may prove beneficial in improving disease resistance. The first study examined the effects of a Lactobacillus probiotic (Flor...

  15. Increasing Fish Oil Levels in Commercial Diets Influence Hematology and Immune Responses of Channel Catfish, Ictalurus punctatus

    Science.gov (United States)

    Cultured freshwater fish including channel catfish are commonly fed grain-soybean meal based feeds high in linoleic series (n-6) fatty acids. Published studies have shown that supplementation of catfish diets with marine fish oil rich in n-3 highly unsaturated fatty acids (n-3 HUFA) significantly in...

  16. Evaluation of corn distillers dried grains with solubles and brewers yeast in diets for channel catfish Ictalurus punctatus

    Science.gov (United States)

    A study was conducted to examine the use of distillers grains with solubles (DDGS), ethanol extracted DDGS (EDDGS), and brewers yeast in channel catfish, Ictalurus punctatus, diets. Diets containing these ingredients were compared with all-plant and fish meal control diets. Juvenile channel catfish ...

  17. Evaluation of potassium permanganate against an experimental subacute infection of Flavobacterium columnare in channel catfish, Icatlurus punctatus

    Science.gov (United States)

    The efficacy of potassium permanganate (KMnO4) as a prophylactic and therapeutic treatment for subacute infection of Flavobacterium columnare was demonstrated in experimentally infected channel catfish, Ictalurus punctatus. Catfish experimentally infected with F. columnare to mimic a subacute infec...

  18. Influence of fertilizing water pH on the hatching success of stripped channel catfish eggs on channel x blue hybrid catfish embryo production in hatcheries

    Science.gov (United States)

    Variable egg quality is one of the most important constrains to the development of aquaculture. The quality of eggs that are manually stripped from channel catfish are affected by variation in parental genetics, maturity, type and dose of hormone, age and pre-spawning stress of female fish. Furthe...

  19. Detection of erythemato-squamous diseases using AR-CatfishBPSO-KSVM

    Directory of Open Access Journals (Sweden)

    Davar Giveki

    2012-01-01

    Full Text Available Nowadays, one of the most important usages of machine learning is diagnosis of diverse diseases. In thiswork, we introduces a diagnosis model based on Catfish binary particle swarm optimization(CatfishBPSO, kernelized support vector machines (KSVM and association rules (AR as our featureselection method to diagnose erythemato-squamous diseases. The proposed model consisted of two stages.In the first stage, AR is used to select the optimal feature subset from the original feature set. Next, basedon the fact that kernel parameter setting in the SVM training procedure significantly influences theclassification accuracy and CatfishBPSO is a promising tool for global searching, a CatfishBPSO basedapproach is employed for parameter determination of KSVM. Experimental results show that the proposedAR-CatfishBPSO-KSVM model achieves 99.09% classification accuracy using 24 features of theerythemato-squamous disease dataset which shows that our proposed method is more accurate comparedto other popular methods in this literature like Support vector machines and AR-MLP (association rules -multilayer perceptron. It should be mentioned that we took our dataset from University of CaliforniaIrvine machine learning database.

  20. Detection of erythemato-squamous diseases using AR-CatfishBPSO-KSVM

    Directory of Open Access Journals (Sweden)

    Davar Giveki

    2011-12-01

    Full Text Available Nowadays, one of the most important usages of machine learning is diagnosis of diverse diseases. In thiswork, we introduces a diagnosis model based on Catfish binary particle swarm optimization(CatfishBPSO, kernelized support vector machines (KSVM and association rules (AR as our featureselection method to diagnose erythemato-squamous diseases. The proposed model consisted of two stages.In the first stage, AR is used to select the optimal feature subset from the original feature set. Next, basedon the fact that kernel parameter setting in the SVM training procedure significantly influences theclassification accuracy and CatfishBPSO is a promising tool for global searching, a CatfishBPSO basedapproach is employed for parameter determination of KSVM. Experimental results show that the proposedAR-CatfishBPSO-KSVM model achieves 99.09% classification accuracy using 24 features of theerythemato-squamous disease dataset which shows that our proposed method is more accurate comparedto other popular methods in this literature like Support vector machines and AR-MLP (association rules -multilayer perceptron. It should be mentioned that we took our dataset from University of CaliforniaIrvine machine learning database.

  1. The efficacy of mass-marking channel catfish fingerlings by immersion in oxytetracycline

    Science.gov (United States)

    Stewart, David R.

    2011-01-01

    Oxytetracycline (OTC) has been extensively used for marking a variety of fish species, but has never been successfully used to mark channel catfish Ictalurus punctatus. Channel catfish fingerlings (~ 25 mm TL) obtained from the Oklahoma Department of Wildlife Conservation at Byron Fish Hatchery were kept in Living Streams (791 to 1,018 L) equipped with recirculation units. Marking trials consisted of immersing channel catfish in one of three concentrations (250, 450, and 700 mg/L) OTC hydrochloride [HCl] for 6 hours. Samples of channel catfish were obtained from each group at 1-week and 4-week postimmersion. Lapilli otoliths and pectoral spines were removed to assess mark presence with an epi-fluorescent compound microscope. After one week, no marks were detected on pectoral spines for all treatments, mark detection on otoliths depended on concentration, but never exceeded 43% (700 mg/L). After four weeks, all otoliths and pectoral spines were determined marked for 700 mg/L OTC, 20% for fish immersed in 450 mg/L OTC, and 0% were marked after four weeks at the 250 mg/L OTC. Results show, channel catfish fingerlings can be successfully marked with immersion in OTC at 700 mg/L for at least 6 hours.

  2. A first generation BAC-based physical map of the channel catfish genome

    Directory of Open Access Journals (Sweden)

    Waldbieser Geoffrey C

    2007-02-01

    Full Text Available Abstract Background Channel catfish, Ictalurus punctatus, is the leading species in North American aquaculture. Genetic improvement of catfish is performed through selective breeding, and genomic tools will help improve selection efficiency. A physical map is needed to integrate the genetic map with the karyotype and to support fine mapping of phenotypic trait alleles such as Quantitative Trait Loci (QTL and the effective positional cloning of genes. Results A genome-wide physical map of the channel catfish was constructed by High-Information-Content Fingerprinting (HICF of 46,548 Bacterial Artificial Chromosomes (BAC clones using the SNaPshot technique. The clones were assembled into contigs with FPC software. The resulting assembly contained 1,782 contigs and covered an estimated physical length of 0.93 Gb. The validity of the assembly was demonstrated by 1 anchoring 19 of the largest contigs to the microsatellite linkage map 2 comparing the assembly of a multi-gene family to Restriction Fragment Length Polymorphism (RFLP patterns seen in Southern blots, and 3 contig sequencing. Conclusion This is the first physical map for channel catfish. The HICF technique allowed the project to be finished with a limited amount of human resource in a high throughput manner. This physical map will greatly facilitate the detailed study of many different genomic regions in channel catfish, and the positional cloning of genes controlling economically important production traits.

  3. Chemical and Microbiology Characteristic of Smoked and Seasoned African Catfish Fillet Affected by Canning Processing

    Directory of Open Access Journals (Sweden)

    Yusuf Kalingga Murda

    2016-08-01

    Full Text Available African catfish (Clarias gariepinus many cultivated by the farmers, however if not treated quicklydamaged. One treatment that can maintain quality product african catfish is by smoked and canning. Theaim of this study was to determine the chemical and microbiological characteristics of smoked Africancatfish fillet with seasoning packaged cans during storage. Preparation of research carried out by soakingthe African catfish fillet into seasonings and liquid smoke concentration of 10% by immersion for 1minute, and then next process of curing and drying. Products that are ripe weighed 110 g and signedinto cans sized Ø 301x205. Added medium brine concentration 5% and vegetable oil as much as 100ml, a process exhausting and seaming. The last stage is performed a sterilization process (126oC for 20minutes, cooling and incubation (24oC for 2 weeks. Observations deterioration of product quality ofsmoked African catfish fillet with seasoning performed at weeks 0, 2, 4, 6 and 8. Tests conducted includechemical test which includes test TVB, pH and peroxide value and microbiological testing in the formof TPC. The results showed that the combined treatment of the fumigation and the addition of medium(saline 5% and solution of vegetable oil combined with treatment canning able to maintain productquality of smoked African catfish fillet with seasoning, based TVB, pH, peroxide value and TPC duringstorage.

  4. Mitochondrial phylogeny and phylogeography of East African squeaker catfishes (Siluriformes: Synodontis

    Directory of Open Access Journals (Sweden)

    Meyer Axel

    2006-06-01

    Full Text Available Abstract Background Squeaker catfishes (Pisces, Mochokidae, Synodontis are widely distributed throughout Africa and inhabit a biogeographic range similar to that of the exceptionally diverse cichlid fishes, including the three East African Great Lakes and their surrounding rivers. Since squeaker catfishes also prefer the same types of habitats as many of the cichlid species, we hypothesized that the East African Synodontis species provide an excellent model group for comparative evolutionary and phylogeographic analyses. Results Our analyses reveal the existence of six major lineages of Synodontis in East Africa that diversified about 20 MYA from a Central and/or West African ancestor. The six lineages show a clear geographic patterning. Two lineages are endemic to Lake Tanganyika (plus one non-endemic representative, and these are the only two Synodontis lineages that diversified further into a small array of species. One of these species is the cuckoo catfish (S. multipunctatus, a unique brood parasite of mouthbrooding haplochromine cichlids, which seems to have evolved in parallel with the radiation of its cichlid host lineage, the Tropheini. We also detect an accelerated rate of molecular evolution in S. multipunctatus, which might be the consequence of co-evolutionary dynamics. Conclusion We conclude that the ancestral lineage of today's East African squeaker catfish fauna has colonized the area before the Great Lakes have formed. This ancestor diversified rapidly into at least six lineages that inhabit lakes and rivers in East Africa. Lake Tanganyika is the only lake harboring a small species flock of squeaker catfishes.

  5. Capability of Catfish (Clarias gariepinus to Accumulate Hg2+ From Water

    Directory of Open Access Journals (Sweden)

    Heny Suseno

    2015-12-01

    Full Text Available Mercury is hazardous contaminant that can be accumulated by aquatic organisms such as fishes, mussels etc. Catfish is one of source of animal protein but it also can accumulate Hg2+ from water that used in aquaculture. Due to less information about capability of catfish to accumulate Hg2+, therefore we studied bioaccumulation of Hg2+ that used biokinetic approach (aqueous uptake-rate, and elimination-rate.  Nuclear application technique was applied in this study by using radiotracer of 203Hg.  A simple kinetic model was then constructed to predict the bioaccumulation capability of   by catfish. The result of experiments were shown that the uptake rate of difference Hg2+ concentration were 79.90 to 101.22 ml.g-1.d-1. Strong correlation between uptake rates with increasing Hg2+concentration. In addition, the elimination rates were range 0.080 – 0.081 day-1. The biology half time (t1/2b of Hg2+ in whole body catfish were 8.50 – 8.63 days.  However, no clear correlation  between elimination rate with increasing concentration of Hg2+. The calculation of Bio Concentration Factor (BCF shown catfish have capability to accumulated Hg maximum 1242.69 time than its concentration in water

  6. Assembly of 500,000 inter-specific catfish expressed sequence tags and large scale gene-associated marker development for whole genome association studies

    Energy Technology Data Exchange (ETDEWEB)

    Catfish Genome Consortium; Wang, Shaolin; Peatman, Eric; Abernathy, Jason; Waldbieser, Geoff; Lindquist, Erika; Richardson, Paul; Lucas, Susan; Wang, Mei; Li, Ping; Thimmapuram, Jyothi; Liu, Lei; Vullaganti, Deepika; Kucuktas, Huseyin; Murdock, Christopher; Small, Brian C; Wilson, Melanie; Liu, Hong; Jiang, Yanliang; Lee, Yoona; Chen, Fei; Lu, Jianguo; Wang, Wenqi; Xu, Peng; Somridhivej, Benjaporn; Baoprasertkul, Puttharat; Quilang, Jonas; Sha, Zhenxia; Bao, Baolong; Wang, Yaping; Wang, Qun; Takano, Tomokazu; Nandi, Samiran; Liu, Shikai; Wong, Lilian; Kaltenboeck, Ludmilla; Quiniou, Sylvie; Bengten, Eva; Miller, Norman; Trant, John; Rokhsar, Daniel; Liu, Zhanjiang

    2010-03-23

    Background-Through the Community Sequencing Program, a catfish EST sequencing project was carried out through a collaboration between the catfish research community and the Department of Energy's Joint Genome Institute. Prior to this project, only a limited EST resource from catfish was available for the purpose of SNP identification. Results-A total of 438,321 quality ESTs were generated from 8 channel catfish (Ictalurus punctatus) and 4 blue catfish (Ictalurus furcatus) libraries, bringing the number of catfish ESTs to nearly 500,000. Assembly of all catfish ESTs resulted in 45,306 contigs and 66,272 singletons. Over 35percent of the unique sequences had significant similarities to known genes, allowing the identification of 14,776 unique genes in catfish. Over 300,000 putative SNPs have been identified, of which approximately 48,000 are high-quality SNPs identified from contigs with at least four sequences and the minor allele presence of at least two sequences in the contig. The EST resource should be valuable for identification of microsatellites, genome annotation, large-scale expression analysis, and comparative genome analysis. Conclusions-This project generated a large EST resource for catfish that captured the majority of the catfish transcriptome. The parallel analysis of ESTs from two closely related Ictalurid catfishes should also provide powerful means for the evaluation of ancient and recent gene duplications, and for the development of high-density microarrays in catfish. The inter- and intra-specific SNPs identified from all catfish EST dataset assembly will greatly benefit the catfish introgression breeding program and whole genome association studies.

  7. Biomarker assessment of the effects of coal strip-mine contamination on channel catfish

    International Nuclear Information System (INIS)

    The paper describes how a suite of biomarkers was used to evaluate acute (1-day) to semichronic (3-month) heavy metal-induced toxicity in channel catfish, lctalurus punctatus, caged at an abandoned strip mine and a noncontaminated reference site. Assays performed include indicators of metabolic, haematological, osmoregulatory, and genotoxic stress. Two cage designs were used to evaluate the importance of exposure routes: one excluding contact with the sediments and the other allowing contact with water and sediments. Significant DNA strand breakage was observed in catfish exposed to both exposure regimens, but evidence of DNA repair was observed only in water-exposed catfish. The transient changes in biomarkers followed by a return to reference values represented an initial stress and an acclimation to normal levels

  8. Electric Organ Discharges of Mormyrid Fish as a Possible Cue for Predatory Catfish

    Science.gov (United States)

    Hanika, S.; Kramer, B.

    During reproductive migration the electroreceptive African sharptooth catfish, Clarias gariepinus (Siluriformes), preys mainly on a weakly electric fish, the bulldog Marcusenius macrolepidotus (Mormyridae; Merron 1993). This is puzzling because the electric organ discharges of known Marcusenius species are pulses of a duration (catfishes' low-frequency electroreceptive system (optimum sensitivity, 10-30Hz Peters and Bretschneider 1981). On the recent discovery that M. macrolepidotus males emit discharges lasting approximately ten times longer than those of females (Kramer 1997a) we determined behavioral thresholds for discharges of both sexes, using synthetic playbacks of field-recorded discharges. C. gariepinus detected M. macrolepidotus male discharges down to a field gradient of 103μVpeak-peak/cm and up to a distance of 1.5m at natural field conditions. In contrast, thresholds for female discharges were not reached with our setup, and we presume the bulldogs eaten by catfish are predominantly male.

  9. A Study on applying the Catfish Biofuel in The Mekong Delta for The Marine Diesel Engine

    Directory of Open Access Journals (Sweden)

    Phan Văn Quân

    2015-12-01

    Full Text Available The manufacturing of Catfish products has been developed rapidly in the Mekong delta. Every year, about 1.2 million tons of Catfish and 150,000 tons of biofuel are produced. The biofuel B100 manufactures in Mekong delta satisfies the America standard ASTM D6751; EURO EN 14214 or Vietnamese standard TCVN 7717. Mekong delta, a lower land area, has a large inland water way system with around 100.000 river boats that operate with marine diesel engine. Using the biofuel for the marine diesel engine in area will reduce the HC, CO, SOx and NOx emission to the environment. Therefore, with a study on applying the catfish biofuel, it will reduce the climate change by the increasing of sea water level and save energy by using green energy to replace petrol oil.

  10. Effects of different heat treatments on lipid quality of striped catfish (Pangasius hypophthalmus

    Directory of Open Access Journals (Sweden)

    Zdzisław Domiszewski

    2011-09-01

    Full Text Available   Background. As a result of consumer acceptance and low price production, sales of striped catfish fillets continue to grow. Striped catfish fillets, due to their white meat and lack of fish scent, can be an alternative to fish such as cod or hake. The paper analysed the influence of four different kinds of heat treatment: boiling with and without the addition of salt, frying, microwave cooking, microwave cooking without water on the composition of fatty acids and the lipid oxidation and hydrolysis level of striped catfish fillets. Material and methods. Assays were performed on striped catfish fillets (Pangasius hypophthalmus, Sauvage 1878, which were bought from local supermarket. Fillets one year before expiration date were assayed. Quality of fish lipids was determined by an analysis of the following factors: peroxide value (PV, anisidine value (AsV, TOTOX value, conjugated dienes (CD, acid value (AV, along with an analysis of the composition of fatty acid (FA via gas chromatography. Results. It was shown that conventional cooking and microwave cooking of striped catfish fillets results in an approximately 10% change in the amount of PUFA, including EPA and DHA, whereas the percentages of SFA and MUFA remain unchanged. The amount of the sum of EPA and DHA in 100 g of raw fillet was 16.5 mg, whereas after conventional cooking, microwave cooking and frying the sum of EPA and DHA was respectively: 12, 22 and 23 mg. It was observed that conventional cooking causes an average 10% loss of fat, a change not observed in case of microwave cooking. In spite of a substantial influence of heat treatment on the amount of both primary and secondary oxidation products, striped catfish lipids maintained good quality after the treatment – PV of every sample was below 3 meq O2/kg lipids, and AsV below 1.5. The addition of salt during boiling caused a 16-fold increase in the amount of peroxides and a fourfold increase in the amount of secondary oxidation

  11. Complete mitochondrial genome of catfish Sperata seenghala (Sykes, 1839) (Siluriformes, Bagridae) from Indus River Sindh, Pakistan.

    Science.gov (United States)

    Lashari, Punhal; Laghari, Muhammad Younis; Xu, Peng; Zhao, Zixia; Jiang, Li; Narejo, Naeem Tariq; Deng, Yulin; Sun, Xiaowen; Zhang, Yan

    2016-01-01

    Complete mitochondrial genome of catfish, Sperata seenghala, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome, which is listed Critically Endangered and red listed species. The complete mitogenome was 16,588 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 31.28% A, 27.80% C, 15.31% G, and 25.57% T. The complete mitochondrial genome of catfish, Sperata seenghala provides the fundamental tools for genetic breeding and conservation studies. PMID:24617486

  12. Feasibility studies of using the Catfish Immune System to produce monoclonal antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Poston, T.M.

    1987-03-01

    The objective of these studies was to determine the feasibility of using a teleost cell line to produce monoclonal antibodies. Studies were undertaken to demonstrate the production of a polyclonal response of channel catfish (Icatalurus punctatus) challenged with mycotoxins coupled to a protein carrier. Companion studies were also performed to induce a permanent cell line with catfish lymphocytes. Attempts to demonstrate a polyclonal response to haptenized mycotoxins were inconclusive. Tests to induce an immortal, permanent cell line with benzene and x-ray irradiated cells were also inconclusive. 3 refs., 13 tabs.

  13. THE PRESENCE OF FLUOROQUINOLONES AS ANTIBIOTIC ON CATFISH : A RISK ASSESSMENT

    OpenAIRE

    Titik Budiati

    2010-01-01

    Catfish is a one of freshwater fish which has good nutrition for human but it can be contaminated by antibiotic such as fluoroquinolones. In risk assessment , the no-observed-effect concentration (NOEC) of fluoroquinolones was considered to be 0.0080 g/kg/day and acceptable daily intake (ADI) for fluoroquinolone is 0.15 μg/kg/day on the basis body weight. There are no reports of toxicity in man or animals from the consumption of fluoroquionolones in catfish and the levels of fluoroquinolones...

  14. Influence of Wind Vector Structure Variation of Typhoon "Catfish" Circulation on Its Pathway Mutation

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    [Objective] The research aimed to study the wind vector structure variation of No.1013 strong typhoon "catfish",and its influence on typhoon pathway mutation.[Method] According to the theory that the asymmetric structure of typhoon vortex had influence on typhoon movement,the center of No.1013 super typhoon "catfish" was as the coordinate origin,and 850,500 hPa tangential rotation speeds within 300-500 km were decomposed into u and v components.The composite force movement tendency of typhoon was analyzed.T...

  15. A Study on applying the Catfish Biofuel in The Mekong Delta for The Marine Diesel Engine

    OpenAIRE

    Phan Văn Quân; Ho Trung Phuoc

    2015-01-01

    The manufacturing of Catfish products has been developed rapidly in the Mekong delta. Every year, about 1.2 million tons of Catfish and 150,000 tons of biofuel are produced. The biofuel B100 manufactures in Mekong delta satisfies the America standard ASTM D6751; EURO EN 14214 or Vietnamese standard TCVN 7717. Mekong delta, a lower land area, has a large inland water way system with around 100.000 river boats that operate with marine diesel engine. Using the biofuel for the marine diesel engin...

  16. Morphometric parameters comparisons of the digestive tract of four teleosts with different feeding habits Comparações de parâmetros morfométricos do trato digestório de quatro teleósteos com diferentes hábitos alimentares

    Directory of Open Access Journals (Sweden)

    Alexssandro Geferson Becker

    2010-04-01

    Full Text Available The present study compared some morphometric parameters of the digestive tract of four teleosts with different feeding habits: traira (Hoplias malabaricus, carnivore, silver catfish (Rhamdia quelen, omnivore, hassar (Hoplosternum littorale, omnivore, and grass carp (Ctenopharyngodon idella, herbivore. The digestive tract was removed from fish collected from nature and fixed for some morphometric analyses, such as: intestinal quotient, intestinal area quotient, vilosity height, digestive somatic index, and hepatosomatic index. Grass carp showed the highest values of intestinal quotient and height vilosity. On the other hand, the intestinal area quotient was higher in traira than in the other species. The intestinal quotient can be used to estimate the feeding habit, and the intestinal area quotient, vilosity height and the relationships between digestive tract length and fish weight or digestive tract weight can provide important additional information to analyze the feeding habits.O presente estudo comparou alguns parâmetros morfométricos do trato digestório de quatro teleósteos com diferentes hábitos alimentares: traira (Hoplias malabaricus, carnivoro, jundiá (Rhamdia quelen, omnivoro, tamoatá (Hoplosternum littorale, omnivoro e carpa capim (Ctenopharyngodon idella, herbivoro. O trato digestório foi removido dos peixes coletados da natureza e em seguida fixado para algumas análises morfométricas, tais como: quociente intestinal, quociente da área intestinal, altura da vilosidade, índice digestivo somático e índice hepatossomático. Os maiores valores para o quociente intestinal e a altura das vilosidades foram encontrados em carpa capim. Por outro lado, o quociente da área intestinal foi maior na traira do que nas outras espécies. O quociente intestinal pode ser utilizado para estimar o hábito alimentar, e o quociente da área intestinal, a altura da vilosidade e as relações entre o comprimento do trato digestório e o peso

  17. Antibacterial Resistance in African Catfish Aquaculture: a Review

    Directory of Open Access Journals (Sweden)

    Madubuike U. ANYANWU

    2016-03-01

    Full Text Available Antibacterial resistance (AR is currently one of the greatest threats to mankind as it constitutes health crisis. Extensive use of antibacterial agents in human and veterinary medicine, and farm crops have resulted in emergence of antibacterial-resistant organisms in different environmental settings including aquaculture. Antibacterial resistance in aquaculture is a serious global concern because antibacterial resistance genes (ARGs can be transferred easily from aquaculture setting to other ecosystems and the food chain. African catfish (ACF aquaculture has increased at a phenomenal rate through a continuous process of intensification, expansion and diversification. Risk of bacterial diseases has also increased and consequently there is increased use of antibacterial agents for treatment. Antibacterial resistance in ACF aquaculture has huge impact on the food chain and thus represents risk to public and animal health. In “one health” approach of curbing AR, knowledge of the sources, mechanisms and magnitude of AR in ACF aquaculture and its potential impact on the food chain is important in designing and prioritizing monitoring programs that may generate data that would be relevant for performing quantitative risk assessments, implementation of antibacterial stewardship plans, and developing effective treatment strategies for the control of ACF disease and reducing risk to public health. This review provides insight on the sources, mechanisms, prevalence and impact of antibacterial resistance in ACF aquaculture environment, a setting where the impact of AR is neglected or underestimated.

  18. Touch sensation by pectoral fins of the catfish Pimelodus pictus.

    Science.gov (United States)

    Hardy, Adam R; Steinworth, Bailey M; Hale, Melina E

    2016-02-10

    Mechanosensation is fundamental to many tetrapod limb functions, yet it remains largely uninvestigated in the paired fins of fishes, limb homologues. Here we examine whether membranous fins may function as passive structures for touch sensation. We investigate the pectoral fins of the pictus catfish (Pimelodus pictus), a species that lives in close association with the benthic substrate and whose fins are positioned near its ventral margin. Kinematic analysis shows that the pectoral fins are held partially protracted during routine forward swimming and do not appear to generate propulsive force. Immunohistochemistry reveals that the fins are highly innervated, and we observe putative mechanoreceptors at nerve fibre endings. To test for the ability to sense mechanical perturbations, activity of fin ray nerve fibres was recorded in response to touch and bend stimulation. Both pressure and light surface brushing generated afferent nerve activity. Fin ray nerves also respond to bending of the rays. These data demonstrate for the first time that membranous fins can function as passive mechanosensors. We suggest that touch-sensitive fins may be widespread in fishes that maintain a close association with the bottom substrate. PMID:26865307

  19. Morphology of the gas bladder in bumblebee catfishes (Siluriformes, Pseudopimelodidae).

    Science.gov (United States)

    Birindelli, José L O; Shibatta, Oscar A

    2011-07-01

    The gross morphology of the gas bladder is described and compared for representatives of all valid genera of Pseudopimelodidae (Siluriformes). Cephalosilurus albomarginatus and species of Batrochoglanis, and Microglanis have the most basic form: a large, cordiform gas bladder with a simple internal T-shaped septum. Cephalosilurus apurensis, C. fowleri, and C. nigricauda also have a large, cordiform gas bladder, but they have well-developed trabeculae associated with the internal T-shaped septum, and a pair of well-developed constrictor muscles inserted on the external wall; the latter feature is present in most species of Pimelodidae, but absent in all other catfishes. The monotypic Lophiosilurus alexandri also has well-developed constrictor muscles, and its gas bladder is moderately sized. The species of Pseudopimelodus and Cruciglanis have a diminutive gas bladder partially divided into two lateral sacs without internal communication, and lack constrictor muscles. The parapophysis of the fourth vertebra is a wide and long shelf connected to the dorsal surface of the gas bladder in most pseudopimelodid genera. However, in the species of Pseudopimelodus and Cruciglanis the parapophysis of the fourth vertebra is shorter and has its anterior ramus folded back, partially covering the gas bladder anteroventrally; and the tympanic opening is smaller than in species of the other genera. Five phylogenetic characters are proposed based on the morphology of the gas bladder and associated structures in species of Pseudopimelodidae, and the evolution of those characters in the family is discussed. J. Morphol., 2011. © 2011 Wiley-Liss, Inc. PMID:21538478

  20. Dietary breadth and trophic position of introduced European catfish Silurus glanis in the River Tarn (Garonne River basin), southwest France

    OpenAIRE

    Syväranta, Jari; Cucherousset, Julien; Kopp, Dorothée; Crivelli, Alain Jean; Céréghino, Régis; Santoul, Frédéric

    2010-01-01

    Although being a widely introduced and successfully established species, the European catfish Silurus glanis L. (the world’s third largest freshwater fish) remains poorly studied in its introduced areas. Here we studied the trophic ecology of non-native European catfish in a large river system in south-western France using stomach content and stable isotope analyses (SIA). We used fin samples for SIA of catfish and hence tested the validity of using fin tissue as a proxy for muscle in SIA. Th...

  1. Evaluation of catfish by-products as protein sources for pigs in the Mekong Delta of Viet Nam

    OpenAIRE

    Thuy, Nguyen Thi

    2010-01-01

    The aim of this study was to evaluate preservation methods of catfish by-products (CBP), and the effects of replacing marine fish meal (FM) with these processing byproducts on diet digestibility, performance, feed efficiency and carcass quality in growing-finishing pigs. Paper I presents data on catfish production, processing and the nutritive value of catfish by-product meals (CBM) in the Mekong Delta (MD) of Vietnam. In Paper II, CBP silage made with 20-40% addition of sugar cane molasses (...

  2. Threadfin shad impacts phytoplankton and zooplankton community structures in channel catfish ponds

    Science.gov (United States)

    Plankton community structure and chlorophyll a concentration were compared in 12 0.1-ha earthen ponds co-stocked with channel catfish (Ictalurus punctatus Rafinesque, 1818) in multiple-batch culture (initial biomass = 5,458 kg ha-1) and a planktivore, threadfin shad (Dorosoma petenense Güther, 1867;...

  3. Overcoming inconsistencies in mortality rates during winter experimental challenges of channel catfish with Edwardsiella ictaluri

    Science.gov (United States)

    One of the goals of the ARS Catfish Genetics Research Unit is to incorporate disease resistance to ESC, caused by the bacterium, Edwardsiella ictaluri, into our selective breeding program. Through repeated experiments we have determined an optimal challenge dose of E. ictaluri that produces 50-70% ...

  4. Utilization of laserpuncture induction as spawning stimulation in catfish (Clarias spp. crossbreeding toward egg quality

    Directory of Open Access Journals (Sweden)

    Pungky S.W. Kusuma

    2015-12-01

    Full Text Available The induction of laserpuncture on the reproductive acupoint of catfish can accelerate gonadotropin hormone formation from the pituitary especially gonadotropin II (GTH-II which has a role in the final stage of oocyte maturation, ovulation and spawning stimulation. The purpose of this study is to evaluate the effects of laserpuncture induction toward the egg quality from crossbreeding catfish male var. Paiton and female var. Sangkuriang. The egg quality was measured by the following parameters: fertilization rate (FR, egg hatching rate (HR, and larvae survival rate (SR. The research treatments were conducted using two levels along with eight repetitions. The results show that the crossbreed catfish using laserpuncture induction affected the parameters by increasing the mean value of fertilization rate, egg hatching rate and larvae survival rate significantly (P < 0.05 compared with mean value of fish without induction. This study concluded that laserpuncture induction on the crossbreeding between broodstock of male catfish var. Paiton and female var. Sangkuriang will increase FR, HR and SR.

  5. Toxicity of organophosphorus pesticide sumithion on larval stages of stinging catfish Heteropneustes fossilis

    Science.gov (United States)

    Shahjahan, Md.; Kabir, Md. Farajul; Sumon, Kizar Ahmed; Bhowmik, Lipi Rani; Rashid, Harunur

    2016-04-01

    Sumithion is widely used to control brittle in paddy fields and tiger bug in fish larval rearing ponds. The objective of this study was to elucidate the toxic effects of sumithion on larval stages of stinging catfish Heteropneustes fossilis. Larvae were exposed to two concentrations (150 and 250 μg/L) of sumithion with one control in three replicates of each. Larvae samples were collected at 20- and 24-h intervals followed by observation under a digital microscope. Exposures of stinging catfish larvae to sumithion produced deformities including irregular head shape, lordosis, yolk sac edema, body arcuation, tissue ulceration, etc. The mortality rates of larvae were significantly increased in response to increase in sumithion concentrations. Furthermore, around 30% of the total adult stinging catfish reared in sumithiontreated aquaculture ponds were found to be deformed permanently. These findings highlight that exposure of stinging catfish to sumithion at the critical and sensitive stages in their life cycle may significantly reduce the number of returning adults. Therefore, the use of sumithion for crop protection needs to be considered carefully and alternatives to sumithion should to be developed for controlling aquatic insects in aqua-ponds during larval rearing.

  6. Prevalence of veterinary drug residues and heavy metals in catfish nuggets

    Science.gov (United States)

    Seafood, including catfish, can sometimes become contaminated with chemicals and heavy metals at levels which would harm human health. Seafood safety has been increasing concerns to the consumers in the United States and limited availability of domestic fresh products in retail stores have been rais...

  7. Global transcription analysis of vaccinated channel catfish following challenge with virulent Edwardsiella ictaluri

    Science.gov (United States)

    To determine the identities of genes involved in either innate or adaptive immunity, microarray analysis of 65,182 UniGene transcripts were performed to compare gene expression in vaccinated channel catfish after challenge with a virulent Edwardsiella ictaluri compared to that in sham-vaccinated fis...

  8. In-pond raceway systems and catfish disease related cases in west Alabama

    Science.gov (United States)

    Production systems such as in-pond raceway systems (IPRS) and split-pond production systems are providing an alternative to traditional pond culture for raising catfish. Currently, there are over 1,300 water acres of production in Mississippi, Arkansas, and Alabama utilizing split-pond production sy...

  9. Plutonium-237: comparative uptake in chelated and non-chelated form by channel catfish (Ictalurus punctatus)

    International Nuclear Information System (INIS)

    Chelation can either enhance or reduce the uptake of ingested plutonium relative to PuOH (monomer) in channel catfish. Reduced uptake of 237Pu-fulvate is due either to the molecular weight of the complex or its stability in metabolic systems. Increased uptake of 237Pu-citrate is attributable to instability of the complex in metabolic systems. (author)

  10. PHYSIOLOGICALLY-BASED TOXICOKINETIC MODELING OF THREE WATERBORNE CHLOROETHANES IN CHANNEL CATFISH, ICTALURUS PUNCTATUS

    Science.gov (United States)

    A physiologically-based toxicokinetic model for fish was used to describe the uptake and disposition of three chlorinated ethanes in channel catfish (Ictalurus punctatus). atfish were simultaneously exposed to 1,1,2,2-tetrachloroethane (TCE), pentachloroethane (PCE), and hexachlo...

  11. RESPIRATORY-CARDIOVASCULAR PHYSIOLOGY AND CHLOROETHANE GILL FLUX IN THE CHANNEL CATFISH ICTALRUS PUNCTATUS

    Science.gov (United States)

    A fish respirometer-metabolism chamber was used to obtain in ivo respiratory-cardiovascular and chloroethane gill flux data on transected channel catfish (Ictalrus punctatus). ethods used for spinal transection, attachment of an oral membrane (respiratory mask), placement and att...

  12. Biomarker assessment of the effects of strip mine contamination on channel catfish

    International Nuclear Information System (INIS)

    A suite of biomarkers were used to evaluate acute (1 day) to semi-chronic (3 month) heavy metal-induced toxicity in channel catfish, Ictalurus punctatus, caged at an abandoned strip mine and a noncontaminated reference site. Assays performed include indicators of metabolic, hematological, osmoregulatory, and genotoxic stress. Two cage designs were utilized to evaluate the importance of exposure routes; one allowing exclusive contact with the water column and the other allowing contact with water and sediments. Significant DNA strand breakage was observed in catfish exposed in both regimes, but DNA repair was observed only in water-exposed catfish. Transient increases in hemoglobin, ALAD, and hematocrit levels were observed at 1 month for both exposure regimes, followed by a return to control levels for the duration of the study. Environmental conditions (i.e., changes in water quality and temperature) may have contributed to the variable plasma chloride and glucose levels observed in all catfish exposed to strip mine wastes. Further analysis of the data with non-metric clustering techniques and correlations with tissue metal residues may yield more insights into causal relationships

  13. Dioxins and other environmental contaminants in catfish from U.S. commerce

    Science.gov (United States)

    The Food, Conservation, and Energy Act of 2008 (Farm Bill), amended the Federal Meat Inspection Act to provide that "catfish, as defined by the Secretary," is an amenable species and therefore subject to continuous inspection by the USDA Food Safety Inspection Service. Since few background data are ...

  14. Economic feasibility of an in-pond raceway system for commercial catfish production in west Alabama

    Science.gov (United States)

    The endeavor of this project was to improve profitability of catfish farming by demonstrating methods to achieve high levels of survival, feed performance, and efficiency in a commercial farm setting. A commercial-scale, in-pond raceway system was constructed in 2007 in a 6.0 ac earthen pond on a ca...

  15. Molecular responses of ceruloplasmin to Edwardsiella ictaluri infection and iron overload in channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    Ceruloplasmin is a serum ferroxidase that carries more than 90% of the copper in plasma and has documented roles in iron homeostasis as well as antioxidative functions. In our previous studies, it has been shown that the ceruloplasmin gene is strongly up-regulated in catfish during challenge with Ed...

  16. Commercial catfish pond water color and fish-eating birds: What is the connection?

    Science.gov (United States)

    This study was designed to test and predict fish-eating bird use and avoidance at catfish production ponds during 2 winters in Arkansas. We measured (1) physical (size, length, width, depth, surface condition, secchi depth), (2) biological (fish size and stocking density, algal species composition ...

  17. Acute toxicity and histopathology of channel catfish fry exposed to peracetic acid

    Science.gov (United States)

    Channel catfish Ictalurus punctatus yolk-sac fry and swim-up fry were exposed to peracetic acid (PAA) for 48h in static toxicity bioassays at 23C. The test water was 217 and 126 mg/L (as CaCO3) total alkalinity and total hardness, respectively. Probit LC50 values were estimated with the trimmed Sp...

  18. Single intravenous and oral dose pharmacokinetics of florfenicol in the channel catfish Ictalurus punctatus

    Science.gov (United States)

    Plasma distribution and elimination of florfenicol in channel catfish were investigated after a single dose (10mg/kg) of intravenous i.v.) or oral administration in freshwater at a mean water temperature of 25.4°C. Florfenicol concentrations in plasma were analyzed by means of liquid chromatography...

  19. Channel catfish, Ictalurus punctatus, chemokine receptor CXCR4 cDNA

    Science.gov (United States)

    Chemokine receptor CXCR4, a member of the G protein-coupled receptor superfamily, binds selectively CXCL12. This protein plays many important roles in immunological as well as pathophysiological functions. In this communication, we identified and characterized the channel catfish CXCR4 transcript....

  20. Biological performance of Asian catfish (Clarias batrachus (Teleostei, Clariidae cultured in recirculating aquaculture system

    Directory of Open Access Journals (Sweden)

    Rahmat Fadhil

    2011-12-01

    Full Text Available The study was conducted to assess the biological performance of Asian catfish in RAS by using4 tanks (3 culture tanks and 1 sedimentation tank measuring 2 m x 1 m x 0.60 m with a volume of 900liters and bio-filter as water purification unit. The numbers of catfish cultured in each tank were as manyas 300 fish i each tank, with a size of 5-8 cm (Tank 1, 8-12 cm (Tank 2 and 12-15 cm (Tank 3,respectively. This study was conducted for 30 days (August-September 2010 by considering the waterquality during experiment, indicates that the RAS are able to provide proper conditions for growth andsurvival of catfish with a survival rate more than 92% on all tank experiments with specific growth ratefrom 0.64 to 0.89% per day and feed conversion rate of about 0.18-0.27. The existence of the impuritiesfrom the fish waste directly influences water quality, but the decline of water quality still at a decent levelfor the culture of catfish. The ANOVA test to the biological performance and water quality showed thatthe difference in the size of fish in each tank has no significant influence between each other.

  1. Oxygen and nitrogen dynamics in split ponds vs. conventional catfish production ponds

    Science.gov (United States)

    The Split Pond aquaculture system (SP) has captured the attention of catfish producers across the southern U.S. The SP represents a lower cost adaptation of Clemson University’s Partitioned Aquaculture System (PAS). The original PAS design relied on slowly rotating paddlewheels to move water throu...

  2. Mitochondrial cytochrome b sequence variations and phylogeny of the East Asian bagrid catfishes

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The mitochondrial DNA cytochrome b gene was sequenced from 8 bagrid catfishes in China. Aligned with cytochrome b sequences from 9 bagrid catfishes in Japan, Korea and Russia retrieved from GenBank, and selected Silurus meridionalis, Liobagrus anguillicauda, Liobagrus reini and Phenacogrammus interruptus as outgroups, we constructed a matrix of 21 DNA sequences. The Kimura's two-parameter distances were calculated and molecular phylogenetic trees were constructed by using the maximum parsimony (MP) and neighbor-joining (NJ) methods. The results show that (i) there exist 3-bp deletions of mitochondrial cytochrome b gene compared with cypriniforms and characiforms; (ii) the molecular phylogenetic tree suggests that bagrid catfishes form a monophyletic group, and the genus Mystus is the earliest divergent in the East Asian bagrid catfishes, as well as the genus Pseudobagrus is a monophyletic group but the genus Pelteobagrus and Leiocassis are complicated; and (iii) the evolution rate of the East Asian bagrids mitochondrial cytochrome b gene is about 0.18%~0.30% sequence divergence per million years.

  3. Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)

    Indian Academy of Sciences (India)

    Vikas Anathy; Thayanithy Venugopal; Ramanathan Koteeswaran; Thavamani J Pandian; Sinnakaruppan Mathavan

    2001-09-01

    A tissue-specific cDNA library was constructed using polyA+ RNA from pituitary glands of the Indian catfish Heteropneustes fossilis (Bloch) and a cDNA clone encoding growth hormone (GH) was isolated. Using polymerase chain reaction (PCR) primers representing the conserved regions of fish GH sequences the 3′ region of catfish GH cDNA (540 bp) was cloned by random amplification of cDNA ends and the clone was used as a probe to isolate recombinant phages carrying the full-length cDNA sequence. The full-length cDNA clone is 1132 bp in length, coding for an open reading frame (ORF) of 603 bp; the reading frame encodes a putative polypeptide of 200 amino acids including the signal sequence of 22 amino acids. The 5′ and 3′ untranslated regions of the cDNA are 58 bp and 456 bp long, respectively. The predicted amino acid sequence of H. fossils GH shared 98% homology with other catfishes. Mature GH protein was efficiently expressed in bacterial and zebrafish systems using appropriate expression vectors. The successful expression of the cloned GH cDNA of catfish confirms the functional viability of the clone.

  4. Changes in health beneficial components during ice storage of African catfish (Clarias gariepinus)

    NARCIS (Netherlands)

    Larsen, R.; Mierke-Klemeyer, S.; Maehre, H.; Schram, E.; Luten, J.B.

    2010-01-01

    Ice-storage is the most common method of preserving fresh fish. The aim of this work was to study whether ice storage had an effect on contents at selenium, taurine and fatty acid composition in farmed African catfish (Clarias gariepinus). Gutted fish (n = 40) were stored in melting ice for 21 days,

  5. Functioning of catfish electroreceptors: Influence of calcium and sodium concentration on the skin potential

    NARCIS (Netherlands)

    Schouten, V.J.A.; Bretschneider, F.

    1980-01-01

    1. 1. The skin potential of catfish was measured in order to test the hypothesis that it controls electroreceptor sensitivity. 2. 2. The skin potential depends on the “milieu extérieur” in the same way as reported lor goldfish (Fig. 2). 3. 3. The variation of the skin potential is very large compa

  6. Isolation and immunohistochemical identification of Flavobacterium psychrophilum from the tissue of catfish, Clarias batrachus.

    Science.gov (United States)

    Verma, Vinay; Prasad, Yogendra

    2014-03-01

    This study deals with the identification of bacteria in paraffin embedded tissue of Indian catfish, Clarias batrachus experimentally infected with F. psychrophilum to ascertain it's precise role in the occurrence and state of carrier stage of coldwater disease in asymptomatic fishes. Significant (P fish pathogenic bacteria in carrier fish where mortality occurs without explicit clinical signs of disease. PMID:24665767

  7. Dietary copper effects survival of channel catfish challenged with Flavobacterium columnare

    Science.gov (United States)

    Columnaris disease is one of the most important bacterial diseases of channel catfish, Ictalurus punctatus, commercially grown in the US. Copper sulfate (CuSO4) has also been shown to be both therapeutic and prophylactic as a water treatment for columnaris disease. As copper is an essential dietar...

  8. Susceptibility of three strains of blue catfish to parasite Ichthyophthirius multifiliis

    Science.gov (United States)

    This study compared the susceptibility of 3 blue catfish (Ictalurus furcatus) strains (D&B, USDA 101 and USDA 102) to the parasite Ichthyopthirius multifiliis (Ich). In Trial I, a cohabitation study (all strains stocked communally) was conducted and fish were exposed to theronts at 0, 200, 1000, 5,...

  9. CHANNEL CATFISH INDUSTRY IN THE USA AND THE OFF-FLAVOR PROBLEM

    Directory of Open Access Journals (Sweden)

    Nikola Fijan

    2000-03-01

    Full Text Available The history, the production technology in channel catfish pond farming industry as well as the statistical data on production, processing and product value during past 15 years are presented. The trend of increasing consumption by the population and the presently low prices of grain and soybeans are conductive to further expansion of production. The steady growth of the industry is stimulated by several factors: innovative efforts by farmers, research at the universities and at government institutions some of which have numerous experimental ponds, cooperative extension service for farmers, modern marketing, activities of catfish farming associations, high quality of products from processing plants and vertical integration. The off-flavor in catfish caused by algal metabolites is a major problem in the industry. Genera of algae producing such metabolites, their accumulation in other fish and occurrence in drinking water reservoirs as well as the current emphasis on preventing the entrance of off-flavor contaminated catfish onto the market were reviewed. The main undesirable algal metyabolites are volatile alcohols geosmin and 2-methylisoborneol (MIB. The need for less expensive and quick methods of identifying major off-flavor compounds was pointed out. Research at the University of Arkansas at Pine Bluff, USA, on control of off-flavor algae in experimental ponds by filter-feeding silver carp (Hypophthalmichthys molitrix Val. and tilapias confined in cages showed this approach to be rather promising.

  10. Impact of weather on off-flavor episodes at a Louisiana commercial catfish farm.

    Science.gov (United States)

    The catfish aquaculture industry is hampered by off-flavor events that affect timely sale and pond restocking. In this study, weather data was correlated with geosmin and 2-methylisoborneol levels in 21 commercial ponds. Samples were collected weekly for 44 weeks. The off-flavor compounds, geosmi...

  11. Expression profiles of seven channel catfish antimicrobial peptides in response to Edwardsiella ictaluri infection

    Science.gov (United States)

    Using quantitative PCR technique, the relative transcriptional levels of seven channel catfish antimicrobial peptide (AMP) genes [NK-lysin type 1, NK-lysin type 2, NK-lysin type 3, bactericidal permeability-increasing protein (BPI), cathepsin D, hepcidin, and liver-expressed antimicrobial peptide 2 ...

  12. The African catfish, Clarias gariepinus, a model for the study of reproductive endocrinology in teleosts

    NARCIS (Netherlands)

    Oordt, P.G.W.J. van; Goos, H.J.Th.

    1987-01-01

    In their natural habitat African catfish, Clarias gariepinus, show a discontinuous reproductive cycle. This cycle follows changes in the gonadotropic activity of the pituitary. Gonadotropin release has been shown to be under dual hypothalamic control, i.e. a gonadotropin-releasing hormone (GnRH) and

  13. Effects of co-stocking smallmouth buffalo, Ictiobus bubalus, with channel catfish, Ictalurus punctatus

    Science.gov (United States)

    Proliferative gill disease (PGD) in catfish is caused by the myxozoan Henneguya ictaluri. The complex life cycle requires Dero digitata as the oligochaete host. Efforts to control PGD by eradicating D. digitata have been unsuccessful. Smallmouth buffalo, Ictiobus bubalus, (SMB) are opportunistic bot...

  14. Sex Genotype and Sex Phenotype Contribute to Growth Differences Between Male and Female Channel Catfish

    Science.gov (United States)

    Channel catfish have an XX:XY genotypic system of sex determination, and until the present study, the influence of sex genotype on growth could not be distinguished from sex phenotype. Genotypic male fish (XY) were produced by mating normal (XX) female fish with YY male fish. A subsample from eac...

  15. Complement regulatory protein genes in channel catfish and their involvement in disease defense response.

    Science.gov (United States)

    Jiang, Chen; Zhang, Jiaren; Yao, Jun; Liu, Shikai; Li, Yun; Song, Lin; Li, Chao; Wang, Xiaozhu; Liu, Zhanjiang

    2015-11-01

    Complement system is one of the most important defense systems of innate immunity, which plays a crucial role in disease defense responses in channel catfish. However, inappropriate and excessive complement activation could lead to potential damage to the host cells. Therefore the complement system is controlled by a set of complement regulatory proteins to allow normal defensive functions, but prevent hazardous complement activation to host tissues. In this study, we identified nine complement regulatory protein genes from the channel catfish genome. Phylogenetic and syntenic analyses were conducted to determine their orthology relationships, supporting their correct annotation and potential functional inferences. The expression profiles of the complement regulatory protein genes were determined in channel catfish healthy tissues and after infection with the two main bacterial pathogens, Edwardsiella ictaluri and Flavobacterium columnare. The vast majority of complement regulatory protein genes were significantly regulated after bacterial infections, but interestingly were generally up-regulated after E. ictaluri infection while mostly down-regulated after F. columnare infection, suggesting a pathogen-specific pattern of regulation. Collectively, these findings suggested that complement regulatory protein genes may play complex roles in the host immune responses to bacterial pathogens in channel catfish.

  16. Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)

    Indian Academy of Sciences (India)

    Vikas Anathy; Thayanithy Venugopal; Ramanathan Koteeswaran; Thavamani J Pandian; Sinnakaruppan Mathavan

    2013-03-01

    A tissue-specific cDNA library was constructed using polyA+ RNA from pituitary glands of the Indian catfish Heteropneustes fossilis (Bloch) and a cDNA clone encoding growth hormone (GH) was isolated. Using polymerase chain reaction (PCR) primers representing the conserved regions of fish GH sequences the 3′ region of catfish GH cDNA (540 bp) was cloned by random amplification of cDNA ends and the clone was used as a probe to isolate recombinant phages carrying the full-length cDNA sequence. The full-length cDNA clone is 1132 bp in length, coding for an open reading frame (ORF) of 603 bp; the reading frame encodes a putative polypeptide of 200 amino acids including the signal sequence of 22 amino acids. The 5′ and 3′ untranslated regions of the cDNA are 58 bp and 456 bp long, respectively. The predicted amino acid sequence of H. fossils GH shared 98% homology with other catfishes. Mature GH protein was efficiently expressed in bacterial and zebrafish systems using appropriate expression vectors. The successful expression of the cloned GH cDNA of catfish confirms the functional viability of the clone.

  17. Effect of chronic, nightly hypoxia on growth and yield of channel x blue hybrid catfish

    Science.gov (United States)

    Performance traits and metabolic responses of the channel x blue hybrid catfish (Ictalurus punctatus female, Jubilee strain x I. furcatus male, D&B strain) in response to chronic nightly hypoxia were evaluated in this 197-d study. Sixteen 0.25-acre earthen ponds were stocked with 6,141 hybrid catfis...

  18. Treating a natural outbreak of columnaris in channel catfish with copper sulfate and potassium permanganate

    Science.gov (United States)

    An F. Columnare-exclusive epizootic occurred in fingerling channel catfish (Ictalurus punctatus) during normal tank culture practices at SNARC. Fish were transferred to the ultra low-flow system and 2.1 mg/L copper sulfate or 3 mg/L potassium permanganate was administered; an untreated control was ...

  19. Effectiveness of copper sulfate and potassium permanganate on channel catfish infected with Flavobacterium columnare

    Science.gov (United States)

    Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were evaluated for their effectiveness to curtail mortality and decrease bacterial load in fish tissues and water in channel catfish Ictalurus punctatus naturally infected with Flavobacterium columnare, the causative agent of columnaris. Fis...

  20. A dynamic simulation model for growth of the African catfish, Clarias gariepinus (Burchell 1822).

    NARCIS (Netherlands)

    Machiels, M.A.M.

    1987-01-01

    In the early '70 it was tried to identify new fish species for aquaculture in Africa. Amongst the most promising candidates was the African catfish. Clariasgariepinus (Burchell 1822). It is an omnivorous fish. which means a wide feeding spectrum. The fish is a partial air breather, so

  1. Pre and postprandial changes in orexigenic and anorexigenic factors in channel catfish Ictalurus punctatus

    Science.gov (United States)

    We examined pre- and postprandial changes in the expression of plasma ghrelin (GHRL) and mRNAs encoding GRLN, cocaine and amphetamine regulated transcript (CART), neuropeptide Y (NPY), and cholecystokinin (CCK) in channel catfish. Fish were either offered feed (Fed) or fasted (Unfed). Feeding incr...

  2. Developing a low cortisol responsive line of channel catfish, Ictalurus punctatus

    Science.gov (United States)

    Nagaraj G. Chatakondi and Brian C. Peterson USDA ARS Warmwater Aquaculture Research Unit, 141 Experiment Station Road, Stoneville, MS 38776. nagaraj.chatakondi@ars.usda.gov Channel catfish, Ictalurus punctatus is the most important farm-raised aquacultured species in the USA. Stressors in aquacultu...

  3. Plasma cortisol stress response in channel catfish, Ictalurus punctatus influences susceptibility to Edwardeseilla ictaluri

    Science.gov (United States)

    Cortisol is a primary stress hormone in fish as its plasma variations correlate with the occurrence of various stressful situations. Past studies have demonstrated that fish subjected to handling stress or poor water quality had a reduced ability to resist pathogens. Channel catfish fingerlings th...

  4. Annual correlative changes in gonads and pituitary gonadotropes of feral African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Oordt, P.G.W.J. van; Peute, J.; Hurk, R. van den; Viveen, W.J.A.R.

    1987-01-01

    The reproductive cycle of male and female African catfish (Clarias gariepinus) can be divided into a breeding period (May–August), a resting period (September–February) and a period of full gametogenesis (March–April). The pituitary gonadotropin (GTH) content and the ultrastructure of the gonadotrop

  5. The regulation of gonadotropin release by neurohormones and gonadal steroids in the African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Leeuw, R. de; Goos, H.J.Th.; Oordt, P.G.W.J. van

    1987-01-01

    The secretion of gonadotropic hormone (GTH) from the pituitary of teleosts is considered to be regulated by neuropeptides and neuroamines of cerebral origin and steroid hormones from the gonads. This paper reviews our studies concerning the control of GTH release in the African catfish, Clarias gari

  6. The South American Mailed Catfishes of the genus Pseudoloricaria Bleeker, 1862 (Pisces, Siluriformes, Loricariidae)

    NARCIS (Netherlands)

    Isbrücker, I.J.H.; Nijssen, H.

    1976-01-01

    Two species of South American Mailed Catfishes of the genus Pseudoloricaria Bleeker, 1862 are redescribed and figured from type-specimens and additional material: Pseudoloricaria laeviuscula (Valenciennes, 1840), and Pseudoloricaria punctata (Regan, 1904). Since the provenance of the holotype of P.

  7. Gender nor sex hormones alter the disease susceptibility of channel catfish to Edwardsiella ictaluri

    Science.gov (United States)

    The use of monosex populations for aquaculture is becoming widely used for several species. Monosex populations are not in wide use in the catfish industry but techniques to develop all male populations have been developed. These studies were conducted to determine if there were any differences be...

  8. Comparison of phytoplankton communities in catfish split-pond aquaculture systems with conventional ponds.

    Science.gov (United States)

    There has been a growing interest and use of variations of partitioned aquaculture systems (PAS) in recent years by the southeastern United States of America farmed catfish industry. Split-pond systems, one type of PAS, are designed to better manage fish waste byproducts (e.g., ammonia) and dissolv...

  9. Molecular Cloning and Sequencing of Channel Catfish, Ictalurus punctatus, Cathepsin H and L cDNA

    Science.gov (United States)

    Cathepsin H and L, a lysosomal cysteine endopeptidase of the papain family, are ubiquitously expressed and involve in antigen processing. In this communication, the channel catfish cathepsin H and L transcripts were sequenced and analyzed. Total RNA from tissues was extracted and cDNA libraries we...

  10. Streptococcal Arthritis, Osteolysis, Myositis, and Spinal Meningitis in Channel Catfish Ictalurus punctatus Broodstock

    Science.gov (United States)

    This report details findings of an investigation into complaints by commercial fingerling producers of low-grade mortalities, poor reproductive success, emaciation, skin lesions, and severely arched backs among broodstock of channel catfish Ictalurus punctatus. Gross lesions involved the jaw, fin ba...

  11. Male-specific DNA markers from African catfish (Clarias gariepinus).

    Science.gov (United States)

    Kovács, B; Egedi, S; Bártfai, R; Orbán, L

    2000-01-01

    We searched for sex-specific DNA sequences in the male and female genomes of African catfish, Clarias gariepinus (Burchell, 1822) by comparative random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Two sex-linked RAPD markers were identified from the male DNA pool and confirmed on individual samples, showing good agreement with phenotypic sex. Both markers were isolated, cloned and characterized. The first marker (CgaY1) was nearly 2.6 kb long, while the length of second one (CgaY2) was 458 bp. Southern blot analysis with a CgaY1 probe showed strong hybridizing fragments only in males and not in females under stringent conditions, indicating the presence of multiple copies of CgaY1 in the male genome. When tested by zoo blot on the genomes of two closely related species from the Clariidae family, CgaY1 hybridized to the DNA of Heterobranchus longifilis and generated a faint male-specific band at low stringency. CgaY2 produced similar hybridization pattern in both sexes of C. gariepinus, C. macrocephalus and H. longifilis. Specific primers were designed to the sequences and the markers were amplified in multiplex PCR reactions together with a control band common to all individuals. This allowed for rapid, molecular sexing of the species on the basis of a simple three band (male) versus one band (female) pattern. According to our knowledge these are the first sex-specific DNA markers isolated from a siluroid fish species. PMID:11766847

  12. Mercury concentrations in gafftopsail catfish and other fishes in waters adjacent to Hobe Sound National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From September 22-26, 1990, 20 gafftopsail catfish (Bagre marinus) and 3 other fish species (n=4) were collected from marine waters adjacent to Hobe Sound National...

  13. Final Critical Habitat for the Beautiful Shiner (Cyprinella formosa), the Yaqui catfish (Ictalurus pricei), and the Yaqui chub (Gila purpurea)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where the final critical habitat for Beautiful Shiner (Cyprinella formosa), the Yaqui catfish (Ictalurus pricei),...

  14. Evaluation of a cohabitation challenge model in immunization trials for channel catfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis

    Science.gov (United States)

    Calcein marking and cohabitation challenge have not been investigated in fish parasite research. This study evaluated a cohabitation challenge method in immunization trials against Ichthyophthirius multifiliis (Ich) using calcein, a fluorescent dye, to mark channel catfish Ictalurus punctatus (Rafi...

  15. INDUCTION OF NITRIC OXIDE SYNTHASE AND ASSOCIATED TOXICITY IN LIVERS OF HARDHEAD CATFISH, ARIUS FELIS, FROM CONTROL AND EPIZOOTIC SITES

    Science.gov (United States)

    Earlier work with a live channel catfish (Ictalurus punctatus) pathogen, Edwardsiella ictaluri, demonstrated the induction of nitric oxide synthase (NOS) in the head kidney, paralleling enteric septicemia (Hawke et al. 1981; Schoor and Plumb 1994). However, another study exposing...

  16. Comparison of stomach contents and condition of two catfish species living at ambient temperatures and in a heated discharge

    International Nuclear Information System (INIS)

    The lower condition factors for two catfish species in water heated by effluents from APCo's Glen Lyn, Virginia plant as compared to control areas probably is not due to lack of food as evidenced by macroinvertebrate sampling and the fact that only 4.3 percent of the catfish stomachs were empty. Results suggested that young-of-the-year channel catfish were nonselectively eating food organisms present at both control and heated stations. The difference between stomach contents of flatheat catfish from the heated site and fauna available at that site indicated that fish were either moving out of the heated area to feed or were selectively feeding on organisms (particularly mayflies) which were in the substrate or drifted into the heated area. Stomach contents of the two species and distribution and abundance of the macroinvertebrate benthos community are detailed

  17. The occurrence of helminth parasites in the gastrointestinal of catfish (Silurus glanis Linnaeus 1758) from the Zarrine-roud river, Iran

    OpenAIRE

    Mohammad Yakhchali; Ali-Asghar Tehrani; Mozafar Ghoreishi

    2012-01-01

    This investigation was undertaken to verify the prevalence of helminths parasites in the gastrointestinal tract of catfish. A total number of 116 catfish (Silurus glanis) were collected from Zarrine-roud river and examined for helminths. Fish were examined after washing contents of gastrointestinal tract and observed for the presence of helminths using a stereo microscope and a light microscope. Results indicated that 18.96% of the examined catfish were infected with digenean trematodes inclu...

  18. Characterization of an Oct1 orthologue in the channel catfish, Ictalurus punctatus: A negative regulator of immunoglobulin gene transcription?

    OpenAIRE

    Miller Norman W; Wilson Melanie R; Kruiswijk Corine P; Ross David A; Hikima Jun-ichi; Lennard Mara L; Warr Gregory W

    2007-01-01

    Abstract Background The enhancer (Eμ3') of the immunoglobulin heavy chain locus (IGH) of the channel catfish (Ictalurus punctatus) has been well characterized. The functional core region consists of two variant Oct transcription factor binding octamer motifs and one E-protein binding μE5 site. An orthologue to the Oct2 transcription factor has previously been cloned in catfish and is a functionally active transcription factor. This study was undertaken to clone and characterize the Oct1 trans...

  19. Effect of Oxytetracycline-Medicated Feed on Antibiotic Resistance of Gram-Negative Bacteria in Catfish Ponds

    OpenAIRE

    DePaola, A.; Peller, J. T.; Rodrick, G E

    1995-01-01

    The effect of oxytetracycline-medicated feeds on antibiotic resistance in gram-negative bacteria from fish intestines and water in catfish ponds was investigated. In experiments in the fall and spring, using ponds with no previous history of antibiotic usage, percentages of tetracycline-resistant bacteria in catfish intestines obtained from medicated ponds increased significantly after 10 days of treatment. In the fall, resistance of the intestinal and aquatic bacteria returned to pretreatmen...

  20. Quality of antimicrobial products used in striped catfish (Pangasianodon hypophthalmus) aquaculture in Vietnam

    OpenAIRE

    Tran Minh Phu; Nguyen Thanh Phuong; Marie-Louise Scippo; Anders Dalsgaard

    2015-01-01

    Antimicrobial usage is common in Asian aquaculture. This study aimed to determine the quality of antimicrobial products used by Vietnamese striped catfish (Pangasianodon hypophthalmus) farmers. Twenty one antimicrobial products (11 products contained a single antimicrobial and 10 products contained a mixture of two different antimicrobials) commonly used by catfish farmers were obtained from so-called chemical shops located in the Mekong Delta, Vietnam. Ultra High Performance Liquid Chromatog...

  1. Concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) of Asian catfish oil by urea complexation: optimization of reaction conditions

    OpenAIRE

    Pornpisanu Thammapat; Sirithon Siriamornpun; Patcharin Raviyan

    2016-01-01

    Optimization of the concentrating conditions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) extracted from Asian catfish oil was studied to obtain a maximum concentration. The crude fish oil was extracted from the belly flap and adipose tissue of Asian catfish, and the extracted oil was used as fresh crude oil. The EPA and DHA were concentrated by the urea complexation method. A hexagonal rotatable design was applied to examine the effects of crystallization temperatur...

  2. Efficacy of oxytetracycline hydrochloride bath immersion to control external columnaris disease on walleye and channel catfish fingerlings

    Science.gov (United States)

    Rach, J.J.; Johnson, Aaron H.; Rudacille, J.B.; Schleis, S.M.

    2008-01-01

    The efficacy of oxytetracycline hydrochloride (OTC-HCl) in controlling external columnaris disease caused by Flavobacterium columnare on fingerling walleyes Sander vitreus and channel catfish Ictalurus punctatus was evaluated in two on-site hatchery trials. Microscopic examination of skin scrapings before treatment confirmed the presence of bacteria with characteristics indicative of F. columnare.in separate trials, walleyes (4.4 g) and channel catfish (1.5 g) were exposed to 60-min static bath treatments of OTC-HCl at 0, 10, and 20 mg/L (walleyes) or 0, 10, 20, and 40 mg/L (channel catfish) on three consecutive days. Each treatment regimen was tested in triplicate, and each replicate contained either 30 walleyes or 55 channel catfish. Posttreatment presumptive disease diagnosis indicated that F. columnare was the disease agent causing the mortality in both species of fish. Walleye survival at 10 d posttreatment was greater in the 10- and 20-mg/L treatment groups than in the control group; however, only the 10-mg/L treatment significantly (P < 0.05) increased walleye survival in comparison with controls. In the channel catfish trial, survival at 10 d posttreatment was significantly (P < 0.05) greater for all OTC-HCl treatment groups relative to controls. Results from these trials indicated that OTC-HCl treatments effectively reduced mortality in walleyes (10 mg/L only) and channel catfish infected with F. columnare. ?? Copyright by the American Fisheries Society 2008.

  3. KANDUNGAN ASAM LEMAK DAN KARAKTERISTIK FISIKO-KIMIA MINYAK IKAN LELE DAN MINYAK IKAN LELE TERFERMENTASI (FATTY ACID CONTENTS AND PHYSICO-CHEMICAL CHARACTERISTICS OF CATFISH OIL AND FERMENTED CATFISH OIL

    Directory of Open Access Journals (Sweden)

    Iskari Ngadiarti

    2013-06-01

    Full Text Available ABSTRACT Catfish oil and fermented catfish oil have not been developed and commercially produced as catfish derived products. Various processing of catfish oil will change both the composition and physico-chemical characteristics. The objective of this study was to identify the composition of fatty acids and physico-chemical characteristics of both catfish oil (MIL and fermented catfish oil (MILT. Results showed that fatty acid composition of catfish oil was MUFA (36.12%> PUFA> (32.43%> SFA (31.45%, while the composition of fermented catfish oil was MUFA (42.96%> SFA (42.32%> PUFA (15.39%. The fermentation process with lactic acid bacteria increased the content of stearic acid but decreased the content of linoleic acid and linolenic acid. Physical and chemical characteristics of MIL and MILT were almost similar, they indicated oxidation process, based on melting point, viscosity, and thiobarbituric acid values. Therefore, in the production of catfish oil or fermented cat fish oil, it is necessary to add spices and/or nutrient as antioxidants sources. Keywords: catfish oil, PUFA, MUFA, SFA and CLA  ABSTRAK Minyak ikan lele (MIL dan minyak ikan lele terfermentasi (MILT sebagai produk turunan dari ikan lele masih belum banyak dikembangkan dan diproduksi secara komersial. Berbagai proses pengolahan minyak dapat mengubah komposisi dan karakteristik fisiko-kimia minyak ikan lele. Tujuan dari penelitian ini adalah untuk mengidentifikasi komposisi asam lemak dan karakteristik fisiko-kimia dari minyak ikan lele (MIL dan minyak ikan lele terfermentasi (MILT. Komposisi asam lemak pada MIL secara berurutan adalah MUFA (36,12% > PUFA (32,43% > SFA (31,45%, sedangkan setelah difermentasi menjadi MILT terjadi perubahan yaitu MUFA (42,96% > SFA (42,32% > PUFA (15,39%. Jenis asam lemak jenuh yang mengalami peningkatan  pada MILT adalah asam lemak stearat, sedangkan asam lemak tidak jenuh ganda yang mengalami penurunan adalah asam linoleat dan linolenat yang

  4. MICROSCOPIC STRUCTURE AND MORPHOMETRIC PARAMETERS OF EUROPEAN CATFISH (SILURIS GLANIS L. SPLEEN

    Directory of Open Access Journals (Sweden)

    O. Dunaevskaya

    2016-03-01

    Full Text Available Purpose. Study of the microscopic structure and morphometric parameters of European catfish spleen. Methodology. For the study, we used the spleen of clinically healthy age-2 European catfish (Silurus glanis L.. We determined the absolute and relative weight of the organ or the index of spleen development. For the histological studies, pieces of the material were preserved in 10-12 % cooled neutral formalin solution, with subsequent filling in paraffin. Paraffin sections were prepared using a sledge microtome, with a thickness of no more than 10 microns. To determine the cell and tissue morphology under light microscopy, we the staining of histological sections with hematoxylin and eosin. Determination of spleen linear dimensions, morphometric measurements were carried out using the "Master of Morphology". Quantitative parameters were analyzed in "Statistica 6.0". Findings. European catfish spleen corresponded to general regularities of fish spleen structure: we identified support-contractile apparatus, white and red pulp and inherent cellular composition. Peculiarities of the microscopic structure of this organ in European catfish is underdevelopment of radial trabeculae, poor development of periarterial lymphoid sheaths, absence of the differentiation to zones in lymphoid nodules. The most developed was red pulp (70.82 ± 10.76%, the least developed — support-contractile apparatus (7.04 ± 0.65%. The base of the white pulp (22.14 ± 6.61% is lymphoid nodules without propagation center. The support-contractile apparatus of the spleen, which includes capsule and trabecular system, is developed unevenly. E.g., the capsule thickness in different parts of the body is not the same, the biggest thickness is in porta, where it attains 21.85 ± 7.44 micrometers, its relative area is 3.06 ± 0.32%. Relative area of the trabecular system is 3.98 ± 2.57%, the most developed are vascular trabeculae. Originality. We found the peculiarities of spleen

  5. Studies on the nutrition of brackish water catfish - Chrysichthys nigrodigitatus . 1. Preliminary investigations on the probable use of vegetable oil in catfish feeds

    OpenAIRE

    Igbinosun, J.E.; Talabi, S.O.

    1983-01-01

    Four groups of brackishwater catfish were fed four diets: N.F. (NIOMR formula 1 feed), A. B. and C. for seven weeks. Feeds N.F., A., B and C. contained 1.21% fish oil + 5.59% vegetable oil; 1.21% fish oil + 7.39% vegetable oil; 1.21% fish oil + 9.09% vegetable oil; 1.21% fish oil + 10.89% vegetable oil respectively. Results of feeding trial showed that growth was best in the group fed diets containing 10.89% vegetable oil and least in those containing 9.09% vegetable oil

  6. Effect of monolaurin and lactic acid on Listeria monocytogenes attached to catfish fillets.

    Science.gov (United States)

    Verhaegh, E G; Marshall, D L; Oh, D H

    1996-04-01

    The purpose of this study was to determine the effects of monolaurin and lactic acid, singly or combined, on Listeria monocytogenes attached to catfish fillets. Skinless catfish fillets were inoculated with L. monocytogenes and dip treated in monolaurin and/or lactic acid solution for various time periods. Results showed that monolaurin up to 400 micrograms/ml had no influence on counts. Conversely, lactic acid-treated fillets had reduced counts compared to controls. Dipping in 0.85, 1.70, or 2.55% lactic acid for 30 min reduced counts by 0.9, 1.4, or 1.3 logs, respectively. Extending the dipping time to 60 min resulted in little additional decrease in counts. Combining monolaurin with lactic acid yielded results similar to lactic acid alone. Hence, population reduction ability resides with lactic acid and not monolaurin. PMID:8796441

  7. The complete mitochondrial genome of the armored catfish, Hypostomus plecostomus (Siluriformes: Loricariidae).

    Science.gov (United States)

    Liu, Shikai; Zhang, Jiaren; Yao, Jun; Liu, Zhanjiang

    2016-05-01

    The complete mitochondrial genome of the armored catfish, Hypostomus plecostomus, was determined by next generation sequencing of genomic DNA without prior sample processing or primer design. Bioinformatics analysis resulted in the entire mitochondrial genome sequence with length of 16,523 bp. The H. plecostomus mitochondrial genome is consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region, showing typical circular molecule structure of mitochondrial genome as in other vertebrates. The whole genome base composition was estimated to be 31.8% A, 27.0% T, 14.6% G, and 26.6% C, with A/T bias of 58.8%. This work provided the H. plecostomus mitochondrial genome sequence which should be valuable for species identification, phylogenetic analysis and conservation genetics studies in catfishes. PMID:25329264

  8. Complete mitochondrial genome of catfish Bagarius bagarius (Hamilton, Sisoridae; Siluriformes) from Indus River Sindh, Pakistan.

    Science.gov (United States)

    Lashari, Punhal; Laghari, Muhammad Younis; Xu, Peng; Zhao, Zixia; Jiang, Li; Narejo, Naeem Tariq; Deng, Yulin; Sun, Xiaowen; Zhang, Yan

    2016-01-01

    Complete mitochondrial genome of fresh water catfish Bagarius bagarius, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome, which is listed Critically Endangered and Red Listed species. The complete mitogenome was 16,457 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 31.14% A, 27.72% C, 15.62% G and 25.50% T. The complete mitochondrial genome of fresh water catfish, B. bagarius provides the basic genetic tools for breeding and conservation program to enhance aquaculture production. PMID:24660912

  9. The complete mitochondrial genome of the armored catfish, Hypostomus plecostomus (Siluriformes: Loricariidae).

    Science.gov (United States)

    Liu, Shikai; Zhang, Jiaren; Yao, Jun; Liu, Zhanjiang

    2016-05-01

    The complete mitochondrial genome of the armored catfish, Hypostomus plecostomus, was determined by next generation sequencing of genomic DNA without prior sample processing or primer design. Bioinformatics analysis resulted in the entire mitochondrial genome sequence with length of 16,523 bp. The H. plecostomus mitochondrial genome is consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region, showing typical circular molecule structure of mitochondrial genome as in other vertebrates. The whole genome base composition was estimated to be 31.8% A, 27.0% T, 14.6% G, and 26.6% C, with A/T bias of 58.8%. This work provided the H. plecostomus mitochondrial genome sequence which should be valuable for species identification, phylogenetic analysis and conservation genetics studies in catfishes.

  10. Histopathological evaluation of seven Amazon species of freshwater ornamental armored catfish

    Directory of Open Access Journals (Sweden)

    Rodrigo Yudi Fujimoto

    2014-08-01

    Full Text Available Fish commonly known as acaris or plecos are freshwater armored catfish economically important as a food resource and as ornamental fish. Most of these species are captured in the Amazon region. However, despite its economic importance, there is a lack of knowledge about their biological aspects. Thus, this study aimed to characterize and evaluate the histopathological aspects of important organs as gills, liver, integument and kidney of seven species of armored freshwater ornamental catfish fromGuamáRiver,Pará State,Brazil. All organs showed typical characteristics of organs of other teleosts. In some species, gills and liver showed slight histopathological changes: telangiectasis, edema and morphological changes related to the presence of parasites (Monogenea and Digenea in the gills, and changes in the arrangement of hepatocytes rows, and vacuolation of hepatocytes in the liver. Thus, the knowledge of the normal structure of organs and changes found can be used as tools for environmental and health monitoring of animals.

  11. Explosive development of pectoral muscle fibres in large juvenile blue catfish Ictalurus furcatus.

    Science.gov (United States)

    Lahiri, S; Fine, M L

    2015-11-01

    As part of an effort on scaling of pectoral spines and muscles, the basis for growth was examined in six pectoral muscles in juvenile blue catfish Ictalurus furcatus, the largest catfish in North America. Fibre number increases slowly in fish from 13.0 to 26.4 cm in total length, doubles by 27.0 cm and remains stable in larger individuals. Simultaneously, mean fibre diameter decreases by half, caused by the addition of new small fibres, before increasing non-linearly in larger fish. The orders of magnitude disparity between the size at hatching and the size of large adults may have selected for rapid muscle fibre addition at a threshold size.

  12. Preliminary study on the natural extenders for artificial breeding of African catfish Clarias gariepinus (Burchell, 1822

    Directory of Open Access Journals (Sweden)

    M. Nor Siti-Azizah

    2010-07-01

    Full Text Available The objective of the present study was to determine the most suitable extender and theirrespective dilution ratios for African catfish sperm for artificial induced breeding and cryopreservationpurposes. Three natural extenders were tested i.e. coconut water, sugarcane water and soybeansolutions, at three different levels of sperm to extender dilutions of 1:20, 1:30 and 1:40. While Ringersolution was used as a control Diluted sperm were fertilized with ready isolated eggs to assess thefertility and hatching rate at 0, 6 and 12 hour intervals. The results showed that the eggs hatchedapproximately 19 to 27 hours after fertilization. In general, the fertilization and hatching rates decreasedwith increasing dilution ratio. With respect to natural extenders, the coconut water showed the highestfertility and hatching rates at 1:20 dilution ratio. Therefore, coconut water at 1:20 dilution ratio was theoptimal condition for African catfish spermatozoa among the natural extenders investigated.

  13. Production of Generation-2 Mekong giant catfish (Pangasinodon gigas cultured with Spirulina sp.

    Directory of Open Access Journals (Sweden)

    Kriangsak Meng-umphan

    2008-11-01

    Full Text Available The purpose of this study is to evaluate the treatment of Spirulina-supplemented pellet feed to 5-year-old F1 groups of Mekong giant catfish (Pangasinodon gigas from the brood stock and intended for use as breeders. The effects on their growth and maturation when cultured in an earthen pond were observed. Results revealed that, compared to control, there was more gain in weight while the feed conversion ratio was lower. The number of red blood cells was also higher while that of white blood cells was lower, compared to control. Out of 18 treated fish (9 males and 9 females, 6 males and 2 females gave sperms and eggs while none from control group did. It was concluded that Spirulina supplemented in pellet feed can improve growth and maturation performance to the brood stock of Mekong giant catfish.

  14. Protein Hydrolysis from Catfish Prepared by Papain Enzyme and Antioxidant Activity of Hydrolyzate

    OpenAIRE

    Ace Baehaki1); Shanti Dwita Lestari; Achmad Rizky Romadhoni

    2015-01-01

    The objective of this research was to make a protein hydrolysates from catfish (Pangasius pangasius) enzymatically using papain enzyme and analyzed the antioxidant activity of protein hydrolysates produced. The research used the method completely randomized design with two replications the treatment were the difference concentration of the papain enzyme (0%, 1%, 2%, 3%, 4%, 5%, and 6%). The parameters of research were antioxidative activity using DPPH (2,2-difenil-1–pikrilhidra...

  15. CHANNEL CATFISH INDUSTRY IN THE USA AND THE OFF-FLAVOR PROBLEM

    OpenAIRE

    Nikola Fijan; Peter W. Perschbacher

    2000-01-01

    The history, the production technology in channel catfish pond farming industry as well as the statistical data on production, processing and product value during past 15 years are presented. The trend of increasing consumption by the population and the presently low prices of grain and soybeans are conductive to further expansion of production. The steady growth of the industry is stimulated by several factors: innovative efforts by farmers, research at the universities and at government ins...

  16. Effect of bait and gear type on channel catfish catch and turtle bycatch in a reservoir

    Science.gov (United States)

    Cartabiano, Evan C.; Stewart, David R.; Long, James M.

    2014-01-01

    Hoop nets have become the preferred gear choice to sample channel catfish Ictalurus punctatus but the degree of bycatch can be high, especially due to the incidental capture of aquatic turtles. While exclusion and escapement devices have been developed and evaluated, few have examined bait choice as a method to reduce turtle bycatch. The use of Zote™ soap has shown considerable promise to reduce bycatch of aquatic turtles when used with trotlines but its effectiveness in hoop nets has not been evaluated. We sought to determine the effectiveness of hoop nets baited with cheese bait or Zote™ soap and trotlines baited with shad or Zote™ soap as a way to sample channel catfish and prevent capture of aquatic turtles. We used a repeated-measures experimental design and treatment combinations were randomly assigned using a Latin-square arrangement. Eight sampling locations were systematically selected and then sampled with either hoop nets or trotlines using Zote™ soap (both gears), waste cheese (hoop nets), or cut shad (trotlines). Catch rates did not statistically differ among the gear–bait-type combinations. Size bias was evident with trotlines consistently capturing larger sized channel catfish compared to hoop nets. Results from a Monte Carlo bootstrapping procedure estimated the number of samples needed to reach predetermined levels of sampling precision to be lowest for trotlines baited with soap. Moreover, trotlines baited with soap caught no aquatic turtles, while hoop nets captured many turtles and had high mortality rates. We suggest that Zote™ soap used in combination with multiple hook sizes on trotlines may be a viable alternative to sample channel catfish and reduce bycatch of aquatic turtles.

  17. Evaluation of red seaweed Gracilaria arcuata as dietary ingredient in African catfish, Clarias gariepinus.

    Science.gov (United States)

    Al-Asgah, Nasser A; Younis, El-Sayed M; Abdel-Warith, Abdel-Wahab A; Shamlol, Faozi S

    2016-03-01

    The aim of this study was to evaluate the use of dried marine seaweed, Gracilaria arcuata for the first time as dietary ingredient in partial substitution of fishmeal on the growth performance, feed utilization and body composition of African catfish, Clarias gariepinus. Four experimental diets were formulated: D1 as a control group; D2; D3 and D4 which included 10%, 20% and 30% G. arcuata meal respectively. One hundred and eighty African catfish weighing 9.62 ± 0.42 g, (mean ± SE) was divided into four groups corresponding to the different feeding regimes. The final body weight of the fishes showed significant differences (P  0.05) were observed between the control group and fishes fed D2 for all previous parameters. Protein productive value, protein efficiency ratio, daily dry feed intake and total feed intake were also significantly lower in fish fed with a diet containing G. arcuata than in the control group and D2 which contains 10% of G. arcuata. Overall, the results of the experiment revealed that African catfish fed a diet with G. arcuata included in 20% and 30% levels showed poorer growth and feed utilization than the control group and D2. However, the study recommended that C. gariepinus can accept this ingredient up to 10% in their diets. More defined experiments therefore seem to be necessary in order to determine the maximum level of this marine seaweed in diets with amino acid supplementation for African catfish.

  18. Comparative in vitro metabolism and activation of benzo(a)pyrene from two species of catfish

    Energy Technology Data Exchange (ETDEWEB)

    Ploch, S.A.; Di Giulio, R.T. [Duke Univ., Durham, NC (United States)

    1995-12-31

    Cytochrome P4501A activity as measured by hepatic ethoxyresorufin-O-deethylase EROD in isolated microsomes is consistently found to be 10 to 20 fold higher in channel catfish (Ictalurus punctuatus) than in a closely related species of catfish, the brown bullhead (Ameriurus nebulosus). To measure more directly the ability of these two species to activate a model PAH, microsomes and hepatocytes isolated from naphthoflavone induced channel catfish (CC) and brown bullhead (BB) were incubated with [{sup 3}H]- or [{sup 14}C]benzo(a)pyrene (BP) to compare metabolite formation and binding to DNA and protein. Microsomes from channel catfish produce 4-fold higher levels of BP metabolites (hexane non-extractable) than brown bullhead (253.0, 9.0 vs 58.8, 14.9 pmole/mg, protein/min). Binding of microsome activated BP to salmon sperm DNA demonstrated a similar 5-fold difference between the two species. Using isolated hepatocytes, the two species demonstrated less pronounced, but consistent differences in metabolite formation (29.85, 2.2 vs 20.1, 0.96 pmole/10{sup 6}, cells/min, CC vs BB), DNA binding and total protein binding (13.2, 2.53 vs 10.57, 1.72 pmole BP/mg, protein/min, CC vs BB). Surprisingly, these preliminary data suggest that induced CC liver activate a model PAH carcinogen more quickly and produce higher levels of stable adducts than a cancer prone species (brown bullhead). Other aspects of BP activation, detoxification and genotoxicity in these two species may contribute to species` susceptibility to carcinogenesis.

  19. Studies on certain aspects of behaviour in the blind catfish Horaglanis krishnai Menon.

    OpenAIRE

    Mercy T.V. Anna; Pillai N. Krishna; Balasubramanian N. K.

    2001-01-01

    Horaglanis krishnai is a blind catfish inhabiting the dug-out wells at Kottayam, Kerala. This fish has great zoogeographical importance as a similar blind clariid, Uegitglanis zammaroni is found only in the artesian wells of the former Italian Somaliland. Studies on certain behavioural aspects of the fish in captive conditions showed that, this fish even though is blind, exhibited a high degree of thigmotactism. Locomotion, comfort behaviour, feeding and light sensitivity of the fish were stu...

  20. Redescription of the Striped Catfish Mystus tengara (Hamilton, 1822) (Siluriformes: Bagridae), India

    OpenAIRE

    A. Darshan; Mahanta, P. C.; Barat, A.; P. Kumar

    2013-01-01

    The Bagrid Catfish Mystus tengara was described from a pond located in India under the genus Pimelodus. The validity of the fish has been confused and has frequently been treated as a synonym of Mystus vittatus. In this study, the species is rediagnosed and redescribed on the basis of fresh material collected from the Ganga-Brahmaputra drainage. M. tengara is diagnosed from congeners in having a unique combination of the following characters: body with a distinct tympanic spot and four bro...

  1. Effect of Enzyme Supplementation in Diet for the Growth of Channel Catfish

    Directory of Open Access Journals (Sweden)

    Shazia Erum

    2014-05-01

    Full Text Available Channel catfish has practically proven to be one of the species with virtually remarkable performance in fresh water pond culture. Due to better growth rate, good adaptation and acceptability towards artificial feed channel catfish was introduced to Pakistan. A study was conducted to see the role of dietary enzyme Laccasse supplementation in a diet (35% CP on growth of fingerlings of channel catfish. For this purpose a six week nutrition study was conducted in Aquaria of Aquaculture and Fisheries program (NARC. Fish was fed a locally prepared (35% CP diet incorporated with three different concentration of Laccasse enzyme i.e., at the rate of 1, 2 and 3 mL/kg. The dietary pellets were prepared and air dried and fed to the fish at the rate of three percent of wet body weight twice daily. Prior to the start of study fish were acclimatized for a period of two weeks on experimental diets. Weights of the fish were recorded at the start and end of trial fortnightly in between. At the end result was deduced through the data of weight gain. The results revealed that Laccasse supplemented at a concentration of 2 mL/kg has higher growth, however it is insignificant (p>0.05 with a concentration of 3 mL/kg. So addition of laccasse in higher concentration or lower concentrations serve no purpose. We concluded that further detailed studies are added to evaluate the role of laccasse enzyme in feed utilization and growth enhancement of channel catfish juveniles.

  2. Responses of Antioxidant Enzymes in Catfish Exposed to Liquid Crystals from E-Waste

    Directory of Open Access Journals (Sweden)

    Hongtao Yu

    2008-06-01

    Full Text Available Liquid crystals (LCs are typically elongated organic molecules with a non-uniform distribution of electrical charges leading to a dipole. LCs are widely used in displays of computers and other electronic devices. The rapid obsolescence rate of electronics results in large amounts of liquid crystal displays (LCDs entering the environment. Data on health effects of LCs on living creatures are currently limited to some acute toxicity tests by a few major LC manufacturers. These tests concluded that the vast majority of LCs are not acutely toxic. Since the amount of LCs in electronic devices is very small, the health effects of LCs at low concentrations or doses become important. Catfish were used as the test animals in this study. Four major enzymes of the fish’s antioxidant defense system catalase (CAT, superoxide dismutase (SOD, selenium-dependent glutathione peroxidase (Se-GPx, and glutathione-Stransferase (GST were chosen as biomarkers to examine effects of LCs, which were taken from obsolete laptop personal computers made in the early 1990s. The catfish were fed with food containing different contents of LCs for 40 days. Activities of the four chosen enzymes in fish livers were assayed. The results showed that there were significant inductions of CAT, SOD, and Se-GPx activities in response to the LC doses. The plots of the enzyme activities versus LC doses suggested an occurrence of oxidative stress when the dose reached about 20 μg LC/g fish·d. It was concluded that LCs can cause pollutant-induced stress to catfish at low doses. CAT, SOD and Se-GPx are effective biomarkers to give early warning on potential health effects of LCs on some aquatic lives including catfish.

  3. Inside the guts of wood-eating catfishes: can they digest wood?

    OpenAIRE

    German, Donovan P.

    2009-01-01

    To better understand the structure and function of the gastrointestinal (GI) tracts of wood-eating catfishes, the gross morphology, length, and microvilli surface area (MVSA) of the intestines of wild-caught Panaque nocturnus, P. cf. nigrolineatus “Marañon”, and Hypostomus pyrineusi were measured, and contrasted against these same metrics of a closely related detritivore, Pterygoplichthys disjunctivus. All four species had anatomically unspecialized intestines with no kinks, valves, or ceca o...

  4. Ituglanis agreste, a new catfish from the rio de Contas basin, northeastern Brazil (Siluriformes: Trichomycteridae)

    OpenAIRE

    Sergio M. Q. Lima; Cinara P. Neves; Rafael M. Campos-Paiva

    2013-01-01

    Ituglanis agreste, a new species of catfish, is described from a tributary stream of the rio Gongogi drainage, rio de Contas basin, Bahia State, northeastern Brazil, from a transition area between the Atlantic Rain Forest and the semi-arid Caatinga savanna. This species is distinguished from its congeners by the following characters: elongate interopercle plate with high number of odontodes (26-30), high number of ribs (5-6), fewer vertebrae (36), number of branchiostegal rays (7), number of ...

  5. Karyotype analysis of an endemic sucker catfish, Glyptothorax silviae Coad,1981 (Actinopterygii: Sisoridae), from Iran

    OpenAIRE

    Esmaeili, Hamid Reza; Gholami, Zeinab; NAZARI, Narges; GHOLAMIFARD, Ali

    2009-01-01

    The karyotypic and cytogenetic characteristics of southern catfish, Glyptothorax silviae Coad, 1981, was investigated for the first time by examining metaphase chromosome spreads obtained from gill epithelial and kidney cells. The diploid chromosome number of this species was 2n = 52. The karyotype consisted of 9 pairs of metacentric, 14 pairs of submetacentric, and 3 pairs of subtelocentric chromosomes. The arm number was 98. No heteromorphic sex chromosomes were cytologically detected in th...

  6. Hypoxia tolerance and partitioning of bimodal respiration in the striped catfish (Pangasianodon hypophthalmus)

    DEFF Research Database (Denmark)

    Lefevre, Sjannie; Huong, Do Thi Thanh; Wang, Tobias;

    2011-01-01

    Air-breathing fish are common in the tropics, and their importance in Asian aquaculture is increasing, but the respiratory physiology of some of the key species such as the striped catfish, Pangasianodon hypophthalmus Sauvage 1878 is unstudied. P. hypophthalmus is an interesting species...... to air-breathe. The possibility of reducing air-breathing frequency may be energetically beneficial for the fish, leaving more of the aerobic scope for growth and other activities, due to the proposed energetic costs of surfacing behavior....

  7. Modeling River Hydrologic Regime and Spawning of Migratory Catfishes in Southeastern Peruvian Amazon

    Science.gov (United States)

    Canas, C. M.; Waylen, P. R.

    2008-05-01

    Seasonal hydrologic conditions and catfish larvae production were evaluated in the Madre de Dios River in order to determine whether environmental conditions influence the reproductive activity of a group of large, commercially important catfishes, in the Southeastern Peruvian Amazon. A simple stochastic model of floods is presented to describe the influence of the natural high flow regime on observed patterns of catfish larvae release and drifting. Daily river stage records at Puerto Maldonado are related to weekly larval catches to determine the association between flood and spawning events. On the basis of hydroclimatologic characteristics of Andean- Amazon regions, available long-term historical rainfall records are employed to approximate the likely inter- annual variability of floods within this Amazon headwater basin. Major larval drift appeared associated with stages of over the 5 m, or "Biologic Hydrologic Significant Events" (BSE), which act as triggers, or a reasonable surrogates, for spawning responses of these species. The timing of BSEs, estimated from the historical rainfall records, appear to be uniformly distributed during the rain season and their inter-arrival times exponential. These observations provided the basis of the stochastic model describing the likelihood of volumes of larvae releases from the headwater region to lowland Amazon. The ecologically significant role of the hydroclimatology of this region in the complete life cycle of this important Amazon fish resource is illustrated.

  8. Sex biased expression of ghrelin and GHSR associated with sexual size dimorphism in yellow catfish.

    Science.gov (United States)

    Zhang, Jin; Ma, Wenge; He, Yan; Wu, Junjie; Dawar, Farman Ullah; Ren, Fan; Zhao, Xiaohan; Mei, Jie

    2016-03-10

    Sexual size dimorphism has been observed in many cultivable fish species including yellow catfish, in which male fish grow much faster than female fish. Ghrelin is a potent stimulator of pituitary growth hormone (GH) release and known to potentially promote food intake and body weight gain. In order to investigate the molecular mechanism of sexual size dimorphism in yellow catfish (Pelteobagrus fulvidraco), ghrelin and its functional receptor, growth hormone secretagogue receptor (GHSR) cDNAs were cloned. Real-time PCR indicated that both ghrelin and GHSR were more highly expressed in hypothalamus and gut of male fish than female. During normal larval development, expression of ghrelin and GHSR genes was significantly higher in males than in females. 17a-Methyltestosterone (MT) treatment enhanced the expression of ghrelin in female larval fish and GHSR in both sexes, whereas the expression of ghrelin in male larval fish increased in the beginning, then decreased as the treatment time prolonged. Furthermore, the expression of ghrelin and GHSR in male juvenile was significantly increased compared with female juvenile, in short and long term fasting periods, suggesting that male fish may have a better appetite than female during fasting. Our results demonstrate that sex difference in the expression of ghrelin and GHSR may be involved in sexual size dimorphism by regulating feeding and GH/IGF signaling in yellow catfish. PMID:26692148

  9. Mitochondrial genome of the shorthead catfish (Pelteobagrus eupogon): structure, phylogeny, and intraspecific variation.

    Science.gov (United States)

    Wang, R-Q; Wang, D-Z; Li, C-T; Yang, X-R

    2016-01-01

    The complete 16,532-nucleotide sequence of the mitochondrial genome of the shorthead catfish (Pelteobagrus eupogon) was determined using the long and accurate polymerase chain reaction method, and compared with the mitochondrial genome sequences of 49 other catfish species belonging to the order Siluriformes. The locations of protein-coding genes and ribosomal ribonucleic acids (RNAs) were identified by comparison with known sequences of other catfishes, including P. fulvidraco and P. nitidus. The P. eupogon mitochondrial genome was composed of 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNA genes, and a non-coding control region. The gene order was identical to that of other Siluriformes. Phylogenetic analyses based on mitochondrial 12S ribosomal RNA, 16S ribosomal RNA, and 13 protein-coding gene sequence data sets were carried out to further clarify the relative phylogenetic position of P. eupogon, and identify phylogenetic relationships among 24 families of Siluriformes. Phylogenetic analyses Randomized Axelerated Maximum Likelihood (RAxML) 8.0.X were congruent with a basal split of the order into Clupeiformes, Characiformes, Cypriniformes, and Siluriformes, and supported a closer relationship of P. eupogon with Amblycipitidae than Siluridae. We therefore concluded that this species appears to be closely related to the Amblycipitidae. In the phylogenetic tree, the Amblycipitidae appeared as the most basal extant lineage within the Siluriformes, while the Bagridae appeared as the sister group of Cranoglanididae and Pangasiidae. The mitochondrial genome sequence of P. eupogon has been deposited in GenBank (accession No. KJ001784). PMID:27323031

  10. Contribution of anadromous fish to the diet of European catfish in a large river system

    Science.gov (United States)

    Syväranta, Jari; Cucherousset, Julien; Kopp, Dorothée; Martino, Aurélia; Céréghino, Régis; Santoul, Frédéric

    2009-05-01

    Many anadromous fish species, when migrating from the sea to spawn in fresh waters, can potentially be a valuable prey for larger predatory fish, thereby efficiently linking these two ecosystems. Here, we assess the contribution of anadromous fish to the diet of European catfish ( Silurus glanis) in a large river system (Garonne, southwestern France) using stable isotope analysis and allis shad ( Alosa alosa) as an example of anadromous fish. Allis shad caught in the Garonne had a very distinct marine δ13C value, over 8‰ higher after lipid extraction compared to the mean δ13C value of all other potential freshwater prey fish. The δ13C values of European catfish varied considerably between these two extremes and some individuals were clearly specializing on freshwater prey, whereas others specialized on anadromous fish. The mean contribution of anadromous fish to the entire European catfish population was estimated to be between 53% and 65%, depending on the fractionation factor used for δ13C.

  11. Impact of a widely cultivated tree (moringa oleifera) on the health of commercially important hybrid catfish

    International Nuclear Information System (INIS)

    Plantations of the tree Moringa oleifera often lead to increase levels of leaf dust in nearby freshwater environments, and there is concern that, this could have a negative impact on catfish, which are important for aquaculture. Therefore, this study, determined the biochemical alterations in serum, liver and kidney of hybrid catfish (Clarias gariepinus ( ) Heterobranchus bidorsalis ( ) exposed to sub-lethal concentrations of M. oleifera leaf dust in a static renewable bioassay system during a medium term exposure period. The fish (mean length, 16.33 cm, mean weight, 9.90 g) were exposed to 0.16, 0.12, 0.08, 0.04 and 0.00 mg/L concentrations of the plant leaf dust in triplicate exposure. After 21-days of exposure period, the fish were sacrificed for the biochemical parameters: glucose, total protein, albumin, globulin, cholesterol, triglyceride, g-glutamyltransferase, alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase. Fish exposed to leaf dust showed significant differences (P0.05), in the liver and kidney, respectively, in the test fish. Other parameters were not significantly different (P>0.05) in their respective tissue-organs. Ipso-facto, the alteration in biochemical parameters of hybrid catfish exposed to M. oleifera leaf dust was concentration dependent with 0.16 mg/L showing the highest negative alterations thus fish exposed to concentrations above 0.16 mg/L for longer durations may suffer impaired health effect. (author)

  12. Survival strategies of sharptooth catfish Clarias gariepinus in desiccating pans in the northern Kruger National Park

    Directory of Open Access Journals (Sweden)

    B.C.W. Van der Waal

    1998-02-01

    Full Text Available Observations in drying out pans showed that small (26-37 cm sharptooth catfish (Clarias gariepinus can hide at the bottom of small pools filled with sticky mud whereas larger fish stay afloat at the surface in larger pools with sloppy mud, where they easily become prey or succumb to heat stress. The inability of larger fish to keep down in the sloppy mud of up to 40 cm depth is the result of their large bulk and high density of the mud. This may indicate a survival advantage for smaller fish in the final dry-out phase of pools and is supported by the presence of only small fish remains in the last drying up pools of dry pans. Another adaptation of smaller fish includes the temporary congregation outside the water enabling concealment under dense vegetation as a means to escape adverse environmental conditions, including high water temperatures and avian predation. The advantage small fish have over larger catfish under these extreme conditions may explain why catfish are known to show a wide variation in growth rate under natural and aquaculture conditions.

  13. Sublethal toxicity of carbofuran on the African catfish Clarias gariepinus: Hormonal, enzymatic and antioxidant responses.

    Science.gov (United States)

    Ibrahim, Ahmed Th A; Harabawy, Ahmed S A

    2014-08-01

    The present study examined the impacts of carbofuran on endocrinology of the catfish, Clarias gariepinus, for the first time and evaluated cortisol (CRT), triiodothyronine (T3), thyroxin (T4), 17β-estradiol (E2) and testosterone (TST) and the oxidative stress markers including SOD, CAT, GSTs, GSH. The toxic effects on the metabolic enzymes, G6PDH and LDH, in addition to lipid peroxidation (LPO) and DNA damage as biomarkers in Nile catfish, to sublethal exposures of carbofuran (0.16 and 0.49mg/L, for 35 days) were studied. Statistically significant differences between selected parameters between control and carbofuran-treated fish were recorded. Carbofuran caused a significant (pcarbofuran-treated fish. Toxicity of carbofuran on liver, kidney, gills, gonads and muscles after 35 days of exposure was found. Glycogen levels showed a highly significant decrease in liver and gills (p0.05) in gonads of treated fish. The two metabolic enzymes G6PDH and LDH in all tissues exhibited significant decreases (pcarbofuran. LPO levels increased significantly (pcarbofuran. There was a significant (pcarbofuran and allow us to consider catfish as a good bioindicator to reflect the endocrine disrupting impacts of carbofuran, and reflect the potential of this pesticide to cause disturbance in antioxidant defense system as well as metabolism and induction of lipid peroxidation (LPO) and DNA damage in contaminated ecosystems.

  14. Precision of channel catfish catch estimates using hoop nets in larger Oklahoma reservoirs

    Science.gov (United States)

    Stewart, David R.; Long, James M.

    2012-01-01

    Hoop nets are rapidly becoming the preferred gear type used to sample channel catfish Ictalurus punctatus, and many managers have reported that hoop nets effectively sample channel catfish in small impoundments (200 ha). Hoop net series were fished once, set for 3 d; then we used Monte Carlo bootstrapping techniques that allowed us to estimate the number of net series required to achieve two levels of precision (relative standard errors [RSEs] of 15 and 25) at two levels of confidence (80% and 95%). Sixteen hoop net series were effective at obtaining an RSE of 25 with 80% and 95% confidence in all but one reservoir. Achieving an RSE of 15 was often less effective and required 18-96 hoop net series given the desired level of confidence. We estimated that an hour was needed, on average, to deploy and retrieve three hoop net series, which meant that 16 hoop net series per reservoir could be "set" and "retrieved" within a day, respectively. The estimated number of net series to achieve an RSE of 25 or 15 was positively associated with the coefficient of variation (CV) of the sample but not with reservoir surface area or relative abundance. Our results suggest that hoop nets are capable of providing reasonably precise estimates of channel catfish relative abundance and that the relationship with the CV of the sample reported herein can be used to determine the sampling effort for a desired level of precision.

  15. Quality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus Aquaculture in Vietnam.

    Directory of Open Access Journals (Sweden)

    Tran Minh Phu

    Full Text Available Antimicrobial usage is common in Asian aquaculture. This study aimed to determine the quality of antimicrobial products used by Vietnamese striped catfish (Pangasianodon hypophthalmus farmers. Twenty one antimicrobial products (11 products contained a single antimicrobial and 10 products contained a mixture of two different antimicrobials commonly used by catfish farmers were obtained from so-called chemical shops located in the Mekong Delta, Vietnam. Ultra High Performance Liquid Chromatography Mass Spectrometry was used to analyze concentration of sulfonamides, trimethoprim, amoxicillin, cefalexin and ciprofloxacin whereas concentrations of florfenicol and doxycycline were analyzed by High Performance Liquid Chromatography with UV detection. Results revealed that only 4/11 products with a single antimicrobial and 2/10 products with a mixture of antimicrobials contained active substances within ±10% of the concentration declared on the product label. Two products with antimicrobial mixtures did not contain any of the declared antimicrobials. Comparing two batches, analysis of 11 products revealed that only one product contained a concentration of active compound that varied with less than 10% in both batches. Several product labels provided inadequate information on how to calculate therapeutic dosage and further stated withdrawal time despite lack of pharmacokinetic data on the antimicrobials in catfish. There is an urgent need to strengthen approval procedures and in particular regularly to monitor the quality of antimicrobials used in Vietnamese aquaculture.

  16. Quality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam.

    Science.gov (United States)

    Phu, Tran Minh; Phuong, Nguyen Thanh; Scippo, Marie-Louise; Dalsgaard, Anders

    2015-01-01

    Antimicrobial usage is common in Asian aquaculture. This study aimed to determine the quality of antimicrobial products used by Vietnamese striped catfish (Pangasianodon hypophthalmus) farmers. Twenty one antimicrobial products (11 products contained a single antimicrobial and 10 products contained a mixture of two different antimicrobials) commonly used by catfish farmers were obtained from so-called chemical shops located in the Mekong Delta, Vietnam. Ultra High Performance Liquid Chromatography Mass Spectrometry was used to analyze concentration of sulfonamides, trimethoprim, amoxicillin, cefalexin and ciprofloxacin whereas concentrations of florfenicol and doxycycline were analyzed by High Performance Liquid Chromatography with UV detection. Results revealed that only 4/11 products with a single antimicrobial and 2/10 products with a mixture of antimicrobials contained active substances within ±10% of the concentration declared on the product label. Two products with antimicrobial mixtures did not contain any of the declared antimicrobials. Comparing two batches, analysis of 11 products revealed that only one product contained a concentration of active compound that varied with less than 10% in both batches. Several product labels provided inadequate information on how to calculate therapeutic dosage and further stated withdrawal time despite lack of pharmacokinetic data on the antimicrobials in catfish. There is an urgent need to strengthen approval procedures and in particular regularly to monitor the quality of antimicrobials used in Vietnamese aquaculture. PMID:25897517

  17. Two New Genera of Fish Blood Flukes (Digenea: Aporocotylidae) from Catfishes in the Peruvian Amazon.

    Science.gov (United States)

    Orélis-Ribeiro, Raphael; Bullard, Stephen A

    2016-06-01

    Cladocaecum tomasscholzi n. gen., n. sp. infects the heart (lumen of ventricle) of driftwood catfish, Ageneiosus inermis Linnaeus, 1766 (Siluriformes: Auchenipteridae) from the Nanay River (Amazon River Basin, near Iquitos, Peru). It differs from all other aporocotylid genera by having a highly branched intestine comprising a central cecum that terminates immediately anterior to the ovary and that has numerous laterally directed diverticula. Kritsky platyrhynchi ( Guidelli, Isaac, and Pavanelli, 2002 ) n. gen., n. comb. (= Plehniella p.) is redescribed based on paratypes plus new specimens collected from the body cavity of the type host (porthole shovelnose catfish, Hemisorubim platyrhynchos Valenciennes, 1840) (Pimelodidae) from the nearby Itaya River. Kritsky differs from Sanguinicola Plehn, 1905 , Plehniella Szidat, 1951 , Nomasanguinicola Truong and Bullard, 2013 , and Cladocaecum by the combination of having a spinous anterior sucker, an intestine comprising 6 asymmetrical ceca, a lanceolate body, a straight vas deferens, an ovary with finger-like lateral projections, a small and spheroid oötype, numerous, minute, spheroid uterine eggs, and separate genital pores. An updated list of hosts, tissues infected, and geographic localities for the catfish blood flukes (9 spp.; 5 genera) is provided. This is the first report of a fish blood fluke infecting a member of Auchenipteridae and first proposal of a new genus of blood fluke (Schistosomatoidea) from South America in 64 yr. It brings the total number of Amazonian fish blood flukes to a mere 4 species.

  18. Two New Genera of Fish Blood Flukes (Digenea: Aporocotylidae) from Catfishes in the Peruvian Amazon.

    Science.gov (United States)

    Orélis-Ribeiro, Raphael; Bullard, Stephen A

    2016-06-01

    Cladocaecum tomasscholzi n. gen., n. sp. infects the heart (lumen of ventricle) of driftwood catfish, Ageneiosus inermis Linnaeus, 1766 (Siluriformes: Auchenipteridae) from the Nanay River (Amazon River Basin, near Iquitos, Peru). It differs from all other aporocotylid genera by having a highly branched intestine comprising a central cecum that terminates immediately anterior to the ovary and that has numerous laterally directed diverticula. Kritsky platyrhynchi ( Guidelli, Isaac, and Pavanelli, 2002 ) n. gen., n. comb. (= Plehniella p.) is redescribed based on paratypes plus new specimens collected from the body cavity of the type host (porthole shovelnose catfish, Hemisorubim platyrhynchos Valenciennes, 1840) (Pimelodidae) from the nearby Itaya River. Kritsky differs from Sanguinicola Plehn, 1905 , Plehniella Szidat, 1951 , Nomasanguinicola Truong and Bullard, 2013 , and Cladocaecum by the combination of having a spinous anterior sucker, an intestine comprising 6 asymmetrical ceca, a lanceolate body, a straight vas deferens, an ovary with finger-like lateral projections, a small and spheroid oötype, numerous, minute, spheroid uterine eggs, and separate genital pores. An updated list of hosts, tissues infected, and geographic localities for the catfish blood flukes (9 spp.; 5 genera) is provided. This is the first report of a fish blood fluke infecting a member of Auchenipteridae and first proposal of a new genus of blood fluke (Schistosomatoidea) from South America in 64 yr. It brings the total number of Amazonian fish blood flukes to a mere 4 species. PMID:26859799

  19. Antibiotic Application and Emergence of Multiple Antibiotic Resistance (MAR) in Global Catfish Aquaculture.

    Science.gov (United States)

    Chuah, Li-Oon; Effarizah, M E; Goni, Abatcha Mustapha; Rusul, Gulam

    2016-06-01

    Catfish is one of the most cultivated species worldwide. Antibiotics are usually used in catfish farming as therapeutic and prophylactic agents. In the USA, only oxytetracycline, a combination of sulfadimethoxine and ormetoprim, and florfenicol are approved by the Food Drug Administration for specific fish species (e.g., catfish and salmonids) and their specific diseases. Misuse of antibiotics as prophylactic agents in disease prevention, however, is common and contributes in the development of antibiotic resistance. Various studies had reported on antibiotic residues and/or resistance in farmed species, feral fish, water column, sediments, and, in a lesser content, among farm workers. Ninety percent of the world aquaculture production is carried out in developing countries, which lack regulations and enforcement on the use of antibiotics. Hence, efforts are needed to promote the development and enforcement of such a regulatory structure. Alternatives to antibiotics such as antibacterial vaccines, bacteriophages and their lysins, and probiotics have been applied to curtail the increasing emergence of antibiotic-resistant bacteria due to the imprudent application of antibiotics in aquaculture. PMID:27038482

  20. Acute toxicity of nitrofurazone to channel catfish Ictalurus punctatus, and goldfish, Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Wise, M.L.; Stiebel, C.L.; Grizzle, J.M.

    1987-01-01

    Nitrofurazone (5-nitro-2-furaldehyde semicarbazone) is a nitrofuran, a group of organic compounds which have inhibitory activity against many Gram-negative and Gram-positive bacteria and against some protozoan parasites. Although not approved by the United States Food and Drug Administration for use with food fish, nitrofurazone has been found effective in fish against external and internal infections by various species of Aeromonas, Pseudomonas and myxobacteria and can be administered either as a food additive or as a bath treatment. Attempts to control the microsporidian parasite Pleistophora ovariae in golden shiners, Notemigonus crysoleucas, with nitrofurazone met with equivocal results. The following experiment was performed to determine acute toxicity, including lesions, of nitrofurazone to channel catfish, Ictalurus punctatus, and goldfish, carassius auratus, fingerlings. Toxicity of nitrofurazone to channel catfish was determined with low dissolved oxygen concentrations (2 mg/L) to simulate conditions frequently encountered in channel catfish culture. Information abut toxic levels of drugs and the lesions occurring in exposed fish is important to determine the safety of treatment levels and the effects of toxic concentrations.

  1. Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus

    Directory of Open Access Journals (Sweden)

    Wilson Melanie

    2003-11-01

    Full Text Available Abstract A bacterial artificial chromosome (BAC library was constructed by cloning HindIII-digested high molecular weight DNA from a gynogenetic channel catfish, Ictalurus punctatus, into the vector pBeloBAC11. Approximately 53 500 clones were arrayed in 384-well plates and stored at -80°C (CCBL1, while clones from a smaller insert size fraction were stored at -80°C without arraying (CCBL2. Pulsed-field gel electrophoresis of 100 clones after NotI digestion revealed an average insert size of 165 kb for CCBL1 and 113 kb for CCBL2. Further characterization of CCBL1 demonstrated that 10% of the clones did not contain an insert. CCBL1 provides a 7.2-fold coverage of the channel catfish haploid genome. PCR-based screening demonstrated that 68 out of 74 unique loci were present in the library. This represents a 92% chance to find a unique sequence. These libraries will be useful for physical mapping of the channel catfish genome, and identification of genes controlling major traits in this economically important species.

  2. Chemotactic Activity of Cyclophilin A in the Skin Mucus of Yellow Catfish (Pelteobagrus fulvidraco and Its Active Site for Chemotaxis

    Directory of Open Access Journals (Sweden)

    Farman Ullah Dawar

    2016-08-01

    Full Text Available Fish skin mucus is a dynamic barrier for invading pathogens with a variety of anti-microbial enzymes, including cyclophilin A (CypA, a multi-functional protein with peptidyl-prolyl cis/trans isomerase (PPIase activity. Beside various other immunological functions, CypA induces leucocytes migration in vitro in teleost. In the current study, we have discovered several novel immune-relevant proteins in yellow catfish skin mucus by mass spectrometry (MS. The CypA present among them was further detected by Western blot. Moreover, the CypA present in the skin mucus displayed strong chemotactic activity for yellow catfish leucocytes. Interestingly, asparagine (like arginine in mammals at position 69 was the critical site in yellow catfish CypA involved in leucocyte attraction. These novel efforts do not only highlight the enzymatic texture of skin mucus, but signify CypA to be targeted for anti-inflammatory therapeutics.

  3. Occurrence of Cryptosporidium species in catfish (Clarias gariepinus) Harvested from two lakes and artificial ponds in Zaria, Northern Nigeria

    Institute of Scientific and Technical Information of China (English)

    Joy Cecilia Atawodi; Armayau Hamisu Bichi

    2013-01-01

    Objective: To determine the occurrence of Cryptosporidium species in catfish (Clarias gariepinus) harvested from two lakes and ponds in Zaria, Kaduna State, Nigeria.Methods:Catfish samples (n=200) from two lakes and (n=200) from two private fish ponds were collected and their gills and gastrointestinal tract samples were analysed for Cryptosporidium oocysts using modified Ziehl-Neelsen staining technique was used, followed by microscopy. Results: Oocysts of Cryptosporidium species were found in all the sampling sites with an overall positivity rate of 49.75%. A comparison between the lakes and ponds as sampling sites revealed a statistically significant (P<0.05) higher percentage of occurrence of Cryptosporidium oocyst from the lakes (39.25%) than from the ponds (10.50%). Conclusions: These findings document for the first time the natural occurrence ofCryptosporidium sp. in catfish intended for human consumption and therefore, underlines the need for public enlightenment to guard against any possible zoonotic transmission.

  4. Interactions between non-native armored suckermouth catfish (Loricariidae: Pterygoplichthys) and native Florida manatee (Trichechus manatus latirostris) in artesian springs

    Science.gov (United States)

    Nico, Leo G.; Loftus, William F.; Reid, James P.

    2009-01-01

    Non-native suckermouth armored catfishes (Loricariidae) of the genus Pterygoplichthys are now common throughout much of peninsular Florida. In this paper, we present preliminary observations on interactions between a Pterygoplichthys species, tentatively identified as P. disjunctivus (Weber, 1991), and endangered native Florida manatees, Trichechus manatus latirostris (Harlan, 1824), in artesian spring systems in Florida's St. Johns River drainage. The introduced catfish have become abundant in spring habitats, sites used by manatees as winter thermal refuges. In the spring runs, Pterygoplichthys regularly attaches to manatees and grazes the epibiota on their skin. On occasion, dozens of Pterygoplichthys congregate on individual manatees. Manatee responses varied widely; some did not react visibly to attached catfish whereas others appeared agitated and attempted to dislodge the fish. The costs and/or benefits of this interaction to manatees remain unclear.

  5. The Neotropical whale catfishes (Siluriformes: Cetopsidae: Cetopsinae, a revisionary study

    Directory of Open Access Journals (Sweden)

    Richard P. Vari

    2005-06-01

    Full Text Available The catfishes of the subfamily Cetopsinae of the Neotropical family Cetopsidae are revised. Four genera, Cetopsidium new genus, Cetopsis, Denticetopsis, and Paracetopsis Bleeker are recognized as valid. Bathycetopsis, Hemicetopsis, and Pseudocetopsis are considered synonyms of Cetopsis and Paracetopsis Eigenmann & Bean and Cetopsogiton synonyms of Paracetopsis. Thirty-seven species are recognized in the Cetopsinae. Cetopsidium includes six species: C. ferreirai, new species, rio Trombetas; C. minutum, Essequibo River; C. morenoi, central and western portions of río Orinoco; C. orientale, coastal rivers of Suriname and French Guiana, and tentatively rio Tocantins and rio Xingu; C. pemon, new species, río Caura, río Caroni, río Meta, and rio Branco; and C. roae, new species, Rupununi River. Cetopsis includes 21 species: C. amphiloxa, río San Juan, río Atrato, and río Patia, western Colombia, and rivers of northwestern Ecuador; C. arcana, new species, rio Tocantins; C. baudoensis, río Baudo; C. caiapo, new species, rio Tocantins; C. candiru, Amazon basin; C. fimbriata, new species, río Truando; C. coecutiens, rio Amazonas, rio Tocantins, and río Orinoco; C. gobioides, upper rio São Francisco, rio Paraná, río Uruguay, and rio Juquiá; C. jurubidae, río Jurubidá; C. montana, new species, western portions of Amazon basin; C. motatanensis, Lago Maracaibo basin; C. oliveirai, Amazon basin; C. orinoco, río Orinoco, río Aroa, and río Yaracuy; C. othonops, río Magdalena and río Sinú; C. parma, western Amazon basin; C. pearsoni, new species, upper portions of rio Madeira; C. plumbea, western portions of rio Amazonas; C. sandrae, new species, rio Tapajós; C. sarcodes, new species, rio Tocantins; C. starnesi, new species, northwestern río de La Plata and southern rio Madeira; and C. umbrosa, new species, western río Orinoco. Cetopsis chalmersi is a synonym of C. gobioides. Cetopsis macroteronema is a synonym of C. plumbea

  6. The Effect of Grain Prices on the U.S. Catfish Industry: Implications of U.S. Ethanol Production

    OpenAIRE

    Zheng, Hualu; Muhammad, Andrew; Herndon, C.W.

    2009-01-01

    This study estimates the relationship between catfish feed prices (per-unit cost) and relevant economic factors such as the price of farm-raised catfish, farm inputs, and feed ingredients, which include com, soybean meal, cottonseed meal and wheat middling. Given changes in these economic factors it was assumed that the responsiveness of feed cost not instantaneous, and a partial-adjustment model was used in estimation. Results show that a one-percent increase in com prices will cause a 0.062...

  7. Prevalence of pansteatitis in African sharptooth catfish, Clarias gariepinus (Burchell), in the Kruger National Park, South Africa.

    Science.gov (United States)

    Huchzermeyer, K David A

    2012-11-09

    Pansteatitis was confirmed in sharptooth catfish, Clarias gariepinus (Burchell), from three main locations within the Kruger National Park (KNP); the Olifants River Gorge, Engelhard Dam on the Letaba River and from the Sabie River in the Sabiepoort. An increasing prevalence of pansteatitis was observed in catfish during repeated samplings from the Olifants Gorge from 2009 to 2011 and co-existence of old and recent lesions indicated on-going incitement of pansteatitis. Only a low prevalence of pansteatitis was observed in catfish sampled from the Olifants River upstream of the Gorge in the KNP and no pansteatitis was observed in catfish sampled from a rain-filled dam not connected to the Olifants River. Common to both the Olifants Gorge and the Sabiepoort is the damming of the rivers in Mozambique to form lakes Massingir and Corumana respectively. Anthropogenic activities resulting in potential pollution of the rivers differ greatly between these two catchments, providing argument against a primary pollution-related aetiology of the pansteatitis found at these two sites. Compared with other sites, analysis of stomach contents of catfish from the Olifants Gorge and the Sabiepoort strongly suggested that consumption of a predominantly fish diet was associated with the development of pansteatitis in these fish. In a farmed population of catfish used as positive control, development of pansteatitis could be ascribed to consumption of rancid fish waste from a trout slaughterhouse. In the Olifants Gorge, alien invasive silver carp, Hypophthalmychthys molitrix (Valenciennes), seasonally migrate upstream out of Lake Massingir to spawn. This schooling species is an obligate phytoplankton feeder with consequent high levels of adipose tissue n-3 polyunsaturated fatty acids. In the Olifants Gorge, at least, this may explain seasonal exposure to levels of polyunsaturated fats in the diets of catfish and crocodiles to which these animals are not adapted. The possible roles of

  8. Toxic effects of mercuric chloride, methylmercuric chloride, and Emisan 6 (an organic mercurial fungicide) on ovarian recrudescence in the catfish

    Energy Technology Data Exchange (ETDEWEB)

    Kirubagaran, R.; Joy, K.P.

    1988-12-01

    Mercurial toxicity in fishes has been focused mainly on tissue uptake and subcellular distribution, nephrotoxicity, development, hatching and survivability of young ones and teratology. Very few studies have been attempted to investigate Hg toxicity on gonadal activity of fishes throughout the breeding season. In a previous investigation the authors have studied the toxic effects of mercuric chloride (HgCl/sub 2/), methylmercuric chloride (CH/sub 3/HgCl) and emisan 6 (an alkoxyalkyl fungicide) on the survival and histology of the kidney of the catfish, Clarias batrachus. The present report deals with toxic effects of these mercurials on ovarian recrudescence in the catfish, an economically important species in the subcontinent.

  9. Biological performance of Asian catfish (Clarias batrachus) (Teleostei, Clariidae) cultured in recirculating aquaculture system

    OpenAIRE

    Rahmat Fadhil; Johari Endan; Farah S. Taip; Muhammad S. b. Hj. Ja’afar

    2011-01-01

    The study was conducted to assess the biological performance of Asian catfish in RAS by using4 tanks (3 culture tanks and 1 sedimentation tank) measuring 2 m x 1 m x 0.60 m with a volume of 900liters and bio-filter as water purification unit. The numbers of catfish cultured in each tank were as manyas 300 fish i each tank, with a size of 5-8 cm (Tank 1), 8-12 cm (Tank 2) and 12-15 cm (Tank 3),respectively. This study was conducted for 30 days (August-September 2010) by considering the waterqu...

  10. Applications of the Addition of extract and cinnamon leaf flour in the Diet on the Quality of Meat of Catfish

    OpenAIRE

    Suardi Laheng; Mia Setiawati; Dedi Jusadi; Muhammad Agus Suprayudi

    2016-01-01

    This study aimed to evaluate the effect of extract and cinnamon (Cinnamomum burmannii) leaf flour in the diet in increasing quality of meat of catfish (Pangsianodon hypopthalmus). Catfish with a weight of 319.64 ± 35.99 g/nets reared in 9 nets with dimension 2x1x1,5 m3 at a density of 15 fish/nets for 60 days of maintenance. The fish were fed with leaves of cinnamon at a dose that was: 0% cinnamon leaf, 0.1% cinnamon leaf extract, and 1% cinnamon leaf flour. The fish were fed 2 ti...

  11. Evidence of Niche Partitioning under Ontogenetic Influences among Three Morphologically Similar Siluriformes in Small Subtropical Streams

    OpenAIRE

    Karine Orlandi Bonato; Clarice Bernhardt Fialho

    2014-01-01

    Ontogenetic influences in patterns of niche breadth and feeding overlap were investigated in three species of Siluriformes (Heptapterus sp., Rhamdia quelen and Trichomycterus poikilos) aiming at understanding the species coexistence. Samplings were conducted bimonthly by electrofishing technique from June/2012 to June/2013 in ten streams of the northwestern state of Rio Grande do Sul, Brazil. The stomach contents of 1,948 individuals were analyzed by volumetric method, with 59 food items iden...

  12. Avaliação da toxicidade dos compostos fipronil, nitrato de chumbo e naftaleno em peixes

    OpenAIRE

    Ransdorf, Wanessa

    2012-01-01

    Resumo: Diante da grande diversidade de compostos quimicos chegando as aguas naturais, e de grande interesse conhecer os efeitos destes nos organismos aquaticos. No Brasil, ainda sao poucos os estudos visando estudar os efeitos dos agentes xenobioticos nas especies endemicas. Nesse sentido, no presente trabalho, sao apresentados os resultados da toxicidade de tres compostos quimicos (fipronil, nitrato de chumbo e naftaleno) em duas especies de peixes nativas: Rhamdia quelen e Astyanax sp, ale...

  13. Ontogenetic development of weberian ossicles and hearing abilities in the African bullhead catfish.

    Directory of Open Access Journals (Sweden)

    Walter Lechner

    Full Text Available BACKGROUND: The weberian apparatus of otophysine fishes facilitates sound transmission from the swimbladder to the inner ear to increase hearing sensitivity. It has been of great interest to biologists since the 19(th century. No studies, however, are available on the development of the weberian ossicles and its effect on the development of hearing in catfishes. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the development of the weberian apparatus and auditory sensitivity in the catfish Lophiobagrus cyclurus. Specimens from 11.3 mm to 85.5 mm in standard length were studied. Morphology was assessed using sectioning, histology, and X-ray computed tomography, along with 3D reconstruction. Hearing thresholds were measured utilizing the auditory evoked potentials recording technique. Weberian ossicles and interossicular ligaments were fully developed in all stages investigated except in the smallest size group. In the smallest catfish, the intercalarium and the interossicular ligaments were still missing and the tripus was not yet fully developed. Smallest juveniles revealed lowest auditory sensitivity and were unable to detect frequencies higher than 2 or 3 kHz; sensitivity increased in larger specimens by up to 40 dB, and frequency detection up to 6 kHz. In the size groups capable of perceiving frequencies up to 6 kHz, larger individuals had better hearing abilities at low frequencies (0.05-2 kHz, whereas smaller individuals showed better hearing at the highest frequencies (4-6 kHz. CONCLUSIONS/SIGNIFICANCE: Our data indicate that the ability of otophysine fish to detect sounds at low levels and high frequencies largely depends on the development of the weberian apparatus. A significant increase in auditory sensitivity was observed as soon as all weberian ossicles and interossicular ligaments are present and the chain for transmitting sounds from the swimbladder to the inner ear is complete. This contrasts with findings in another otophysine

  14. Bioaccumulation factor for 32P measured in bluegill, Lepomis macrochirus, and catfish, Ictalurus punctatus

    International Nuclear Information System (INIS)

    The ratio of the bioaccumulation factors for 32P and phosphorus was determined for edible tissue in two species of freshwater fish by measuring the specific activity (32P activity per milligram phosphorus) in muscle relative to feed. The 32P tracer was added to the feed at a uniform level throughout the study. Feeding was at two levels: ad libitum and at a lower but constant intake per body weight. In the main experiment, bluegill were maintained in a large flow-through tank and sacrificed at approximately weekly intervals for 51 d of 32P accumulation and 28 d of depuration to compare the specific activity with values predicted with a calculational model. In experiments performed in smaller aquaria, the specific activity in bluegill and catfish muscle was compared at two feeding levels and two temperatures. In addition, unfed fish were exposed to 32P in water at a known specific activity to determine the extent of phosphorus uptake directly from water. The pattern of specific activity increase and decrease in fish muscle during the accumulation/depuration experiment was consistent with a one-compartment model, so that specific activity ratios at steady state could be predicted from measurements during relatively brief exposures. On this basis, the ratio of the bioaccumulation factors of 32P and phosphorus in fish feeding ad libitum was 0.081 for bluegill and 0.17 for catfish. Hence, at a mean phosphorus bioaccumulation factor of 70,000, the factors for 32P are 6000 and 12,000, respectively. The ratios were less at lower phosphorus intakes associated with lower feeding rates; moreover, the lesser value for bluegill occurred at a much lower phosphorus intake than by catfish

  15. Optimizing de novo transcriptome assembly and extending genomic resources for striped catfish (Pangasianodon hypophthalmus).

    Science.gov (United States)

    Thanh, Nguyen Minh; Jung, Hyungtaek; Lyons, Russell E; Njaci, Isaac; Yoon, Byoung-Ha; Chand, Vincent; Tuan, Nguyen Viet; Thu, Vo Thi Minh; Mather, Peter

    2015-10-01

    Striped catfish (Pangasianodon hypophthalmus) is a commercially important freshwater fish used in inland aquaculture in the Mekong Delta, Vietnam. The culture industry is facing a significant challenge however from saltwater intrusion into many low topographical coastal provinces across the Mekong Delta as a result of predicted climate change impacts. Developing genomic resources for this species can facilitate the production of improved culture lines that can withstand raised salinity conditions, and so we have applied high-throughput Ion Torrent sequencing of transcriptome libraries from six target osmoregulatory organs from striped catfish as a genomic resource for use in future selection strategies. We obtained 12,177,770 reads after trimming and processing with an average length of 97bp. De novo assemblies were generated using CLC Genomic Workbench, Trinity and Velvet/Oases with the best overall contig performance resulting from the CLC assembly. De novo assembly using CLC yielded 66,451 contigs with an average length of 478bp and N50 length of 506bp. A total of 37,969 contigs (57%) possessed significant similarity with proteins in the non-redundant database. Comparative analyses revealed that a significant number of contigs matched sequences reported in other teleost fishes, ranging in similarity from 45.2% with Atlantic cod to 52% with zebrafish. In addition, 28,879 simple sequence repeats (SSRs) and 55,721 single nucleotide polymorphisms (SNPs) were detected in the striped catfish transcriptome. The sequence collection generated in the current study represents the most comprehensive genomic resource for P. hypophthalmus available to date. Our results illustrate the utility of next-generation sequencing as an efficient tool for constructing a large genomic database for marker development in non-model species. PMID:25979246

  16. Does the hearing sensitivity in thorny catfishes depend on swim bladder morphology?

    Directory of Open Access Journals (Sweden)

    Angelika Zebedin

    Full Text Available BACKGROUND: Thorny catfishes exhibit large variations in swim bladder morphology. These organs are of different sizes, forms and may have simple or branched diverticula. The swim bladder plays an important role in otophysans because it enhances their hearing sensitivity by transmitting sound pressure fluctuations via ossicles to the inner ear. METHODOLOGY/PRINCIPAL FINDINGS: To investigate if a form-function relationship exists, the swim bladder morphology and hearing ability were analyzed in six species. The morphology was quantified by measuring the length, width and height and calculating a standardized swim bladder length (sSBL, which was then used to calculate the relative swim bladder length (rSBL. Hearing was measured using the auditory evoked potential (AEP recording technique. Two species had simple apple-shaped and four species heart-shaped (cordiform bladders. One of the latter species had short unbranched diverticula on the terminal margin, two had a secondary bladder and two had many long, branched diverticula. The rSBL differed significantly between most of the species. All species were able to detect frequencies between 70 Hz and 6 kHz, with lowest thresholds found between 0.5 and 1 kHz (60 dB re 1 µPa. Hearing curves were U-shaped except in Hemidoras morrisi in which it was ramp-like. Mean hearing thresholds of species possessing smaller rSBLs were slightly lower (maximum 8.5 dB than those of species having larger rSBLs. CONCLUSIONS/SIGNIFICANCE: The current findings reveal a relationship between swim bladder form and its function among thorny catfishes. Relatively smaller swim bladders resulted in relatively better hearing. This is in contrast to a prior inter-familial study on catfishes in which species with large unpaired bladders possessed higher sensitivity at higher frequencies than species having tiny paired and encapsulated bladders.

  17. Otocinclus batmani, a new species of hypoptopomatine catfish (Siluriformes: Loricariidae) from Colombia and Peru

    OpenAIRE

    Pablo Lehmann A

    2006-01-01

    A new species of the hypoptopomatine catfish genus Otocinclus is described from two localities in the upper Río Amazonas basin: a tributary of the Río Puré in Colombia and two tributaries to the Río Amazonas near Iquitos in Peru. The new taxon can be easily distinguished from all congeners, except Otocinclus cocama, by having a single, intensely pigmented, vertical W-shaped caudal fin spot and by having three discrete dark bands on dorsum, between the dorsal-fin base and the caudal fin. Otoci...

  18. ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate

    OpenAIRE

    Mahmoodani, Fatemeh; Ghassem, Masomeh; Babji, Abdul Salam; Yusop, Salma Mohamad; Khosrokhavar, Roya

    2012-01-01

    Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cut-off (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study ind...

  19. Functioning of catfish electroreceptors: Influence of calcium and sodium concentration on the skin potential

    OpenAIRE

    Schouten, V.J.A.; Bretschneider, F.

    1980-01-01

    1. 1. The skin potential of catfish was measured in order to test the hypothesis that it controls electroreceptor sensitivity. 2. 2. The skin potential depends on the “milieu extérieur” in the same way as reported lor goldfish (Fig. 2). 3. 3. The variation of the skin potential is very large compared with the normal stimulus range of electroreceptors. 4. 4. Calcium strongly influences the skin potential, but the latter “adapts” to calcium concentrations of 0.3-3.0 mM (Fig. 3). 5. 5. Ion-depen...

  20. Culture possibility of scheilbeid catfish using formulated feed in natural pond

    OpenAIRE

    Razzaque, M. A.; Mazid, M.A.; M. N. Islam; Mansur, M.A.

    2008-01-01

    A research study was conducted to investigate the culture possibility of fresh water catfish Pangasius pangasius with formulated feed in natural ponds. The stocking density was chosen as 8000 fry/ha with an initial weight of 42.57+ 2.51 g. The formulated feed contained 45% fishmeal, 30% mustard oil cake, 15% wheat bran and 10% rice bran to supply 40.48% protein in feed. The growth of Pangasius pangasius in terms of net weight gain was found as 266.49g, which was statistically significant (p

  1. Biological information of Taunayia bifasciata (Siluriformes: Heptapteridae): a threatened and unknown catfish

    OpenAIRE

    Giulianna Rondineli; Alberto Luciano Carmassi; Francisco Manoel de Souza Braga

    2011-01-01

    Taunayia bifasciata (Eigenmann & Norris, 1900) is a small catfish that inhabits headwater streams of the Tietê and Paraíba do Sul river basins, southeastern Brazil, being restricted to the Atlantic rain forest. The species is found on lists of threatened species of Brazil and the state of São Paulo. Despite that, there is no literature information about the biology of the species. In the present study we endeavored to collect data on the biology of T. bifasciata. A total of 37 specimens were ...

  2. Efficacy of crystalline lysine in alternative diets for pond-raised hybrid catfish, female Ictalurus punctatus X male Ictalurus furcatus

    Science.gov (United States)

    study was conducted to examine the efficacy of crystalline lysine in alternative diets for pond-raised hybrid catfish, ' Ictalurus punctatus × ' Ictalurus furcatus. Two 28% protein alternative diets supplemented with l-lysine HCl at the required level based on 62% (previously published value) or 10...

  3. Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection.

    Science.gov (United States)

    Song, Lin; Li, Chao; Xie, Yangjie; Liu, Shikai; Zhang, Jiaren; Yao, Jun; Jiang, Chen; Li, Yun; Liu, Zhanjiang

    2016-02-01

    Heat shock proteins 70/110 (Hsp70/110) are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions. Besides the chaperone and housekeeping functions, they are also known to be involved in immune response during infection. In this study, we identified 16 Hsp70/110 geness in channel catfish (Ictalurus punctatus) through in silico analysis using RNA-Seq and genome databases. Among them 12 members of Hsp70 (Hspa) family and 4 members of Hsp110 (Hsph) family were identified. Phylogenetic and syntenic analyses provided strong evidence in supporting the orthologies of these HSPs. In addition, we also determined the expression patterns of Hsp70/110 genes after Flavobacterium columnare and Edwardsiella ictaluri infections by meta-analyses, for the first time in channel catfish. Ten out of sixteen genes were significantly up/down-regulated after bacterial challenges. Specifically, nine genes were found significantly expressed in gill after F. columnare infection. Two genes were found significantly expressed in intestine after E. ictaluri infection. Pathogen-specific pattern and tissue-specific pattern were found in the two infections. The significantly regulated expressions of catfish Hsp70 genes after bacterial infections suggested their involvement in immune response in catfish. PMID:26693666

  4. Post-prandial protease activity in the digestive tract of African catfish, Clarias gariepinus, larvae fed decapsulated cysts of Artemia

    NARCIS (Netherlands)

    García Ortega, A.; Verreth, J.; Segner, H.

    2000-01-01

    The alkaline proteolytic activity in the gut of African catfish larvae was studied during short time ranges from 30 min to 4 h after ingestion of decapsulated Artemia cysts. The variation in total protease and trypsin activities during the day was monitored during starvation, after one single meal i

  5. Impacts of Bolbophorus damnificus digenea: Bolbophoridae on production characteristics of channel catfish, Ictalurus punctatus, raised in experimental ponds

    Science.gov (United States)

    Bolbophorus damnificus was recognized as a threat to channel catfish, Ictalurus punctatus, production in Louisiana and Mississippi in 1997 and 1999, respectively. Severe to moderate outbreaks (=34% prevalence) are correlated to heavy losses, but impact of mild outbreaks with low prevalence (<33%) wa...

  6. Adjusting to trade-policy changes in export markets : evidence from U.S. antidumping duties on Vietnamese catfish

    OpenAIRE

    Brambilla, Irene; Porto, Guido; Tarozzi, Alessandro

    2009-01-01

    A large literature studies the effects of trade policy changes on developing-country exports on household incomes, and recent contributions have increasingly addressed the effects of administered protection, such as anti-dumping duties. In 2003 the United States imposed anti-dumping tariffs on imports of catfish from Vietnam ranging from 37 to 64 percent. As a result, Vietnamese exports of...

  7. GROWTH AND SURVIVAL OF CHANNEL CATFISH AND YELLOW PERCH EXPOSED TO LOWERED CONSTANT AND DIURNALLY FLUCTUATING DISSOLVED OXYGEN CONCENTRATIONS

    Science.gov (United States)

    Growth and survival were determined for duplicate lots of juvenile channel catfish (Ictalurus punctatus) and yellow perch (Perca flavescens) exposed for 69 and 67 days, respectively, to nearly constant dissolved oxygen (DO) concentrations at near air saturation (control), 6.5, 5....

  8. Replacement of fish meal in juvenile channel catfish, Ictalurus punctatus, diets using a yeast-derived protein source

    Science.gov (United States)

    We examined the effects of a yeast-derived protein source (NuPro) as a replacement for menhaden fish meal on weight gain, specific growth rate (SGR), food conversion ratio (FCR), whole-body composition, and disease resistance in juvenile channel catfish. NuPro replaced 0, 20, 40, 60, 80, and 100% o...

  9. HISTOPATHOLOGICAL EVIDENCE OF REGENERATION FOLLOWING HEPATOTOXIC EFFECTS OF THE CYANOTOXIN MICROCYSTIN-LR IN THE HARDHEAD CATFISH AND GULF KILLIFISH.

    Science.gov (United States)

    The cyanobacterial toxin, microcystin-LR (MC-LR), causes liver damage in several freshwater fish species. The damage appears to be acute and irreversible. In the present study, two estuarine species, hardhead catfish, Arius felis, and gulf killifish, Fundulus grandis, were inject...

  10. Can reduced stocking rates and natural forage utilization produce market sized catfish from fingerlings in one growing season

    Science.gov (United States)

    The purpose of this study was to determine the feasibility of growing marketable channel catfish Ictalurus punctatus from pond-run fingerlings (15.9 g/fish) using low stocking densities (7,413 or 14,826 fish/ha) and electrified bug lights to enhance natural forage available to fish. Even at low sto...

  11. JAK and STAT members of yellow catfish Pelteobagrus fulvidraco and their roles in leptin affecting lipid metabolism.

    Science.gov (United States)

    Wu, Kun; Tan, Xiao-Ying; Xu, Yi-Huan; Chen, Qi-Liang; Pan, Ya-Xiong

    2016-01-15

    The present study clones and characterizes the full-length cDNA sequences of members in JAK-STAT pathway, explores their mRNA tissue expression and the biological role in leptin influencing lipid metabolism in yellow catfish Pelteobagrus fulvidraco. Full-length cDNA sequences of five JAKs and seven STAT members, including some splicing variants, were obtained from yellow catfish. Compared to mammals, more members of the JAKs and STATs family were found in yellow catfish, which provided evidence that the JAK and STAT family members had arisen by the whole genome duplications during vertebrate evolution. All of these members were widely expressed across the eleven tissues (liver, white muscle, spleen, brain, gill, mesenteric fat, anterior intestine, heart, mid-kidney, testis and ovary) but at the variable levels. Intraperitoneal injection in vivo and incubation in vitro of recombinant human leptin changed triglyceride content and mRNA expression of several JAKs and STATs members, and genes involved in lipid metabolism. AG490, a specific inhibitor of JAK2-STAT pathway, partially reversed leptin-induced effects, indicating that the JAK2a/b-STAT3 pathway exerts main regulating actions of leptin on lipid metabolism at transcriptional level. Meanwhile, the different splicing variants were differentially regulated by leptin incubation. Thus, our data suggest that leptin activated the JAK/STAT pathway and increases the expression of target genes, which partially accounts for the leptin-induced changes in lipid metabolism in yellow catfish. PMID:26704851

  12. Simulated Impacts of Climate Change on Current Farming Locations of Striped Catfish (Pangasianodon hypophthalmus; Sauvage) in the Mekong Delta, Vietnam

    NARCIS (Netherlands)

    Nguyen, L.A.; Dang, V.H.; Bosma, R.H.; Verreth, J.A.J.; Leemans, R.; Silva, De S.S.

    2014-01-01

    In Vietnam, culturing striped catfish makes an important contribution to the Mekong Delta's economy. Water level rise during rainy season and salt intrusion during dry season affect the water exchange and quality for this culture. Sea level rise as a consequence of climate change will worsen these i

  13. Use of corn gluten feed and cottonseed meal to replace soybean meal in diets for pond raised channel catfish

    Science.gov (United States)

    The prices of soybean meal and corn he two most commonly used, traditional feed ingredients in channel catfish diets ave increased dramatically in recent years. Using less-expensive alternative feed ingredients to partially replace soybean meal and corn would reduce feed cost. The present study eval...

  14. Effects of Ichthyophthirius multifiliis parasitism on the survival, hematology and bacterial load in channel catfish previously exposed to Edwardsiella ictaluri

    Science.gov (United States)

    The effect of Ichthyophthirius multifiliis (Ich) parasitism on survival, hematology and bacterial load in channel catfish, Ictalurus punctatus, previously exposed to Edwardsiella ictaluri was studied. Fish were exposed to E. ictaluri one day prior to Ich in the following treatments: 1)- infected by...

  15. Edwardsiella tarda and Aeromonas hydrophila isolated from diseased Southern flounder (Paralichthys lethostigma) are virulent to channel catfish and Nile tilapia

    Institute of Scientific and Technical Information of China (English)

    Julia W Pridgeon; Phillip H Klesius; Gregory A Lewbart; Harry V Daniels; Megan Jacob

    2014-01-01

    Objective:To identify bacteria isolated from diseased Southern flounder and determine whether they are virulent to channel catfish and Nile tilapia. Methods:Gram-negative bacteria isolates were recovered from five tissues of diseased Southern flounder (Paralichthys lethostigma). The isolates were subjected to biochemical and molecular identification followed by virulence study in fish. Results: Based on biochemical analysis, the 25 isolates were found to share homologies with either Edwardsiella tarda (E. tarda) or Aeromonas hydrophila (A. hydrophila). Based on sequencing results of partial 16S rRNA gene, 15 isolates shared 100%identities with the 16S rRNA sequence of previously identified E. tarda strain TX1, whereas the other 10 isolates shared 100%identities with the 16S rRNA sequence of previously identified A. hydrophila strain An4. When healthy fish were exposed to flounder isolate by intracoelomic injection, the LD50 values of flounder isolate E. tarda to channel catfish or Nile tilapia [(10±2) g] were 6.1í104 and 1.1í107 CFU/fish, respectively, whereas that of flounder isolate A. hydrophila to channel catfish and Nile tilapia were 1.4í107 and 5.6í107 CFU/fish, respectively. Conclusions: This is the first report that E. tarda and A. hydrophila isolated from diseased Southern flounder are virulent to catfish and tilapia.

  16. Degradation of chitin and chitosan by a recombinant chitinase derived from a virulent Aeromonas hydrophila isolated from diseased channel catfish

    Science.gov (United States)

    A chitinase was identified in extracellular products of a virulent Aeromonas hydrophila isolated from diseased channel catfish (Ictalurus punctatus). Bioactive recombinant chitinase (rChi-Ah) was produced in Escherichia coli. Purified rChi-Ah had optimal activity at temperature of 42°C and pH 6.5. T...

  17. Partial and total fish meal replacement by agricultural products in the diets improve sperm quality in African catfish (Clarias gariepinus)

    NARCIS (Netherlands)

    Nyina-wamwiza, L.; Milla, S.; Pierrard, M.A.; Rurangwa, E.; Mandiki, S.N.M.; Look, van K.J.W.; Kestemont, P.

    2012-01-01

    This study investigated the long-term effects of total and partial replacement of dietary fish meal (FM) by a mixture of agricultural products on sperm quality of African catfish Clarias gariepinus. Four isonitrogenous and isoenergetic diets were formulated containing graded levels of either 50% FM

  18. The impact of changing dietary Na/K ratio's in growth and nutrient utilisation in juvenile African Catfish Clarias Gariepinus

    NARCIS (Netherlands)

    Dersjant-Li, Y.; Sheng Wu, W.; Verstegen, M.W.A.; Schrama, J.W.; Verreth, J.A.J.

    2001-01-01

    The effects of dietary Na/K ratios on feed intake, growth, nutrient utilisation, plasma and body mineral concentration in juvenile African catfish were investigated. Four Na/K ratios, 0.2, 0.7, 1.5, 2.5 (mmol/mmol), were tested in a randomised experimental design with three replications in each trea

  19. Effect of supplemental taurine on juvenile channel catfish Ictalurus punctatus growth and survival after challenge with Edwardsiella ictaluri

    Science.gov (United States)

    Juvenile channel catfish, Ictalurus punctatus, were fed a basal diet that contained major protein (soybean meal, cottonseed meal) and energy (ground corn grain) ingredients that were derived from plant sources. Plant-source ingredients are considered to be low (taurine content. In add...

  20. Retention of health beneficial components during hot- and cold-smoking of African catfish (Clarias gariepinus) fillets

    NARCIS (Netherlands)

    Larsen, R.; Mierke-Klemeyer, S.; Maehre, H.; Elvevoll, E.O.; Bandarra, N.M.; Cordiero, A.R.; Nunes, M.L.; Schram, E.; Luten, J.B.; Oehlenschlaeger, J.

    2010-01-01

    Changes in content of selenium and taurine, and the alteration of fatty acid profile have been studied in African catfish fillets subjected to a commercial cold- or hot smoking process. Selenium content and the fatty acid profile did not change significantly during neither of the smoking procedures.

  1. Neoplastic response in Japanese medaka and channel catfish exposed to N-methyl-N'-nitro-N-nitrosoguanidine.

    Science.gov (United States)

    Chen, H C; Pan, I J; Tu, W J; Lin, W H; Hong, C C; Brittelli, M R

    1996-01-01

    Japanese medaka (Oryzias latipes) and channel catfish (Ictalurus punctatus) were investigated for carcinogenic response following a 28-day, 3 x/wk pulse exposure to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Five-wk-old medaka were exposed at concentrations of 0, 0.5, and 1.0 mg/L, and 5-mo-old catfish at concentrations of 0, 0.1, and 0.5 mg/L. In medaka, a total of 19 tumors including 2 branchioblastomas, 6 thyroid follicular adenomas and 1 adenocarcinoma, and 11 subcutaneous fibrosarcomas were observed in 16 of 96 MNNG-exposed fish. In catfish, a total of 37 tumors including 4 squamous cell carcinomas and 16 papillomas, 3 lipomas, 1 fibroma, 1 osteosarcoma, 4 branchioblastomas, 6 thymic epithelial tumors, and 2 generalized lymphosarcomas were observed in 34 of 172 MNNG-exposed fish. The induction of neoplasms in medaka was primarily in the gill, thyroid, and subcutis of the cervical and trunk regions, whereas in catfish skin, thymus, oro-pharynx, and hemopoietic tissues were also commonly affected. In both species, the neoplastic response was considered to be related to direct exposure of the tissues to MNNG. Some of these tumors have not been reported in the literature in either natural or experimental fish. The results also suggest species-specific differences in carcinogenic response following MNNG exposure. PMID:8994296

  2. Relationship between expression of muscle-specific uncoupling protein 2 messenger RNA and genetic selection toward growth in channel catfish.

    Science.gov (United States)

    Kobayashi, Y; Peterson, B C; Waldbieser, G C

    2015-04-01

    This study tested the hypothesis that increased growth in channel catfish is associated with expression of the genes that code for uncoupling proteins (UCP) 2 and 3, members of the mitochondrial channel proteins involved in nutrient sensing and metabolism. The specific objective was to contrast the levels of UCP2 messenger RNA (mRNA) in fast vs slow growing catfish as well as in fed vs fasted catfish. Two distinct UCP2 transcripts were identified and named UCP2a and UCP2b, respectively. Nucleotide and amino acid sequence of catfish UCP2s were highly similar to UCP2 and other UCPs from other fish and mammals (>75%). Expression of UCP2a mRNA was detectable at very low levels in various metabolically active tissues, whereas the expression of UCP2b mRNA was readily detectable in the muscle and heart. In a 21-wk feeding study, fish that grew faster had a greater percent body fat at the end of the study (P muscle was increased (P growth and associated fat accumulation appears to be independent of muscle UCP2b mRNA expression and UCP2b-mediated mechanisms.

  3. Two new species and a new genus of neotropical mailed catfishes of the subfamily Loricariinae Swainson, 1838 (Pisces, Siluriformes, Loricariidae)

    NARCIS (Netherlands)

    Isbrücker, I.J.H.; Nijssen, H.

    1978-01-01

    A new monotypic genus and two new species of South American mailed catfishes of the subfamily Loricariinae are described and figured. A discussion of and comparative notes on related taxa are added. Ricola genus novum is established for the species originally described by Regan (1904) as Loricaria (

  4. NRAMP, TNF, TLR5, and Hepcidin Expression in Resistant and Susceptible Families of Channel Catfish Following Challenge With E. ictaluri

    Science.gov (United States)

    Real-time PCR was used to measure gene expression of Nramp, TNF, TLR5, and Hepcidin, in spleen and liver tissue from two families of channel catfish, one resistant and one susceptible to ESC, following challenge with Edwardsiella ictaluri. There were no significant differences in relative copy numbe...

  5. Effects of 17a-methyltestosterone on seminal vesicle, development and semen release response in the African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Viveiros, A.T.M.; Eding, E.H.; Komen, J.

    2001-01-01

    The effects of 17alpha-methyltestosterone on seminal vesicle development in the African catfish, Clarias gariepinus, were investigated in an attempt to improve semen collection from this species. Treatment of larvae with dietary 17alpha-methyltestosterone at 50 mg kg(-1) for days 12-33 or days 12-40

  6. Seasonal changes in steroid metabolism in the male reproductive organ-system of the African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Resink, J.W.; Schoonen, W.G.E.J.; Hurk, R. van den; Viveen, W.J.A.R.; Lambert, J.G.D.

    1987-01-01

    Steroid and steroid glucuronide synthesis in feral male African catfish was investigated in vitro by incubating testes with [3H]-pregnenolone and seminal vesicles with [3H]-androstenedione. In testes, the capacity to form progestins, androgens, especially 11-oxygenated ones, and steroid glucuronides

  7. Steroidogenesis in the testes and seminal vesicles of spawning and non-spawning African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Schoonen, W.G.E.J.; Granneman, J.C.M.; Lambert, J.G.D.; Oordt, P.G.W.J. van

    1987-01-01

    The in vitro biosynthesis of steroids was studied in testes as well as seminal vesicles of non-spawning pond and spawning feral African catfish, collected during the breeding season. In testes of non-spawners the conversion of [3H]-pregnenolone was directed towards 11-oxygenated androgens and 5β-pre

  8. Gelatin hydrolysates from farmed Giant catfish skin using alkaline proteases and its antioxidative function of simulated gastro-intestinal digestion.

    Science.gov (United States)

    Ketnawa, Sunantha; Martínez-Alvarez, Oscar; Benjakul, Soottawat; Rawdkuen, Saroat

    2016-02-01

    This work aims to evaluate the ability of different alkaline proteases to prepare active gelatin hydrolysates. Fish skin gelatin was hydrolysed by visceral alkaline-proteases from Giant catfish, commercial trypsin, and Izyme AL®. All antioxidant activity indices of the hydrolysates increased with increasing degree of hydrolysis (Pskin, could serve as a potential source of functional food ingredients for health promotion. PMID:26304317

  9. Effect of Lactobacillus plantarum C014 on Innate Immune Response and Disease Resistance against Aeromonas hydrophila in Hybrid Catfish

    Directory of Open Access Journals (Sweden)

    Sureerat Butprom

    2013-01-01

    Full Text Available A bacterial strain isolated from intestines of hybrid catfish (Clarias gariepinus Male × Clarias macrocephalus Female exhibited an in vitro inhibitory effect on a fish pathogen, Aeromonas hydrophila TISTR 1321. By using the 16S rDNA sequence analysis, it was identified as Lactobacillus plantarum C014. To examine whether L. plantarum C014 had potential for use as an immunostimulant and biocontrol agent in hybrid catfish, the fish diet supplemented with L. plantarum C014 (107 CFU/g diet was prepared and used for the in vivo investigation of its effect on innate immune response and disease resistance of hybrid catfish. Two innate immune response parameters, phagocytic activity of blood leukocytes and plasma lysozyme activity, were significantly enhanced in the treated fish after 45 days of feeding. Feeding the fish with the L. plantarum C014 supplemented diet for 45 days before challenging them with A. hydrophila at the dose of LD50 could reduce the mortality rate of the fish from 50% (in control group to 0% (in treated group. Based on its origin and beneficial effect on innate immune response and disease resistance, L. plantarum C014 may be a potential candidate for use as a natural and safe immunostimulant and biocontrol agent in hybrid catfish.

  10. An in vitro screening method to evaluate chemicals as potential chemotherapeutants to control Aeromonas hydrophila infection in channel catfish

    Science.gov (United States)

    Using catfish gill cells G1B and four chemicals (hydrogen peroxide, sodium chloride, potassium permanganate, and D-mannose), the feasibility of using an in vitro screening method to identify potential effective chemotherapeutants was evaluated in this study. In vitro screening results revealed that,...

  11. Comparative effects of copper sulfate or potassium permanganate on channel catfish concurrently infected with Flavobacterium columnare and Ichthyobodo necator

    Science.gov (United States)

    An opportunistic study was conducted to determine the effects of two chemical therapeutants on channel catfish (CCF) Ictalurus punctatus concurrently infected Flavobacterium columnare and Ichthyobodo necator. Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were investigated for their abil...

  12. Effects of density, PVC-tubes and feeding time on growth, stress and aggression in African catfish (Clarias gariepinus)

    NARCIS (Netherlands)

    Boerrigter, J.G.J.; Bos, van den R.; Vis, van de J.W.; Spanings, T.; Flik, G.

    2016-01-01

    Successive exposure to aquaculture-related stressors may compromise the allostatic capacity of African catfish and lead to allostatic overload and poor welfare. Therefore, we tested the effect of (i) feeding during the light or dark phase, (ii) density (51 fish per 140 L versus 51 fish per 43 L) and

  13. Rapid screening of oxytetracycline residue in catfish muscle by dispersive liquid-liquid microextraction and europium-sensitized luminescence

    Science.gov (United States)

    Oxytetracycline (OTC) residue in catfish muscle was screened by dispersive liquid-liquid microextraction (DLLME) and europium-sensitized luminescence (ESL). After extraction in EDTA, HCl, and acetonitrile, cleanup was carried out by DLLME, and ESL was measured at microgram = 385 nm and wavelength = ...

  14. Draft genome sequence of Pseudomonas mosselii Gil3, isolated from catfish and antagonistic against hypervirulent Aeromonas hydrophila

    Science.gov (United States)

    Pseudomonas mosselii Gil3 was isolated from a catfish that survived from lethal challenge with hypervirulent Aeromonas hydrophila (vAh). When assayed in vitro, the bacterium showed antagonism against vAh. Sequence analysis revealed that the genome of P. mosselii Gil3 encodes numerous aromatic metabo...

  15. Organic acid dipping of catfish fillets: effect on color, microbial load, and Listeria monocytogenes.

    Science.gov (United States)

    Bal'a, M F; Marshall, D L

    1998-11-01

    Microbiological and color changes of catfish fillets were determined following dip treatment in solutions at 4 degrees C of 2% acetic, citric, hydrochloric, lactic, malic, or tartaric acid. Fillets were inoculated with an eight-strain mixture of Listeria monocytogenes prior to dipping. L. monocytogenes, coliform, and aerobic plate counts and surface pH and Hunter color were measured at 0, 2, 5, and 8 days of storage at 4 degrees C. Acid dipping reduced surface pH and L. monocytogenes, coliform, and aerobic microbial loads. Little microbial proliferation was observed on acid-treated fillets, however, controls had a distinct foul odor and microbial loads in excess of 10(6) CFU/g by day 8. On untreated fillets, L. monocytogenes counts did not increase during storage, perhaps due to competitive inhibition by normal catfish microflora. Hunter color analysis revealed lighter and yellower acid-treated fillets than untreated controls, with malic acid producing the least bleaching. The shelf life of refrigerated fillets increased when fillets were acid dipped. It remains to be established if this enhanced microbial quality also parallels sensory acceptability. PMID:9829187

  16. Use of solar energy in the production of channel catfish. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Cox, G C

    1976-01-01

    No catfish are grown commercially in eastern Kentucky due primarily to a growing season approximately three weeks too short to provide the minimum 180 day feeding season. This research project was designed to test the feasibility of using solar energy to heat pond water enough to lengthen the feeding season the required number of days. The procedure involved the heating of a small starter pond by covering it with a solar swimming pool cover. Fingerling catfish were stocked in the small pond in early April and transferred to a larger rearing pond in May when water temperatures in the larger pond reached 65/sup 0/ F. Oxygen levels were maintained at acceptable levels in the covered pond by frequent aeration. Water quality resulting from a prolonged spring drought and run-off from a pasture fertilized by chicken manure seriously affected the project. Other significant problems encountered included algae build-up and the accumulation ammonia and carbon dioxide in the water. Losses to fungus infection was the most serious problem encountered. Approximately 30 percent of the fingerlings died from this cause while in the small covered pond. After the fish were transferred to the large rearing pond, no further unanticipated problems were encountered. From the time the fish were transferred to the large pond they were handled in a typical open pond operation. At the end of the growing season in October, the fish had an average weight of 1.4 pounds.

  17. Intestinal synthesis and absorption of vitamin B-12 in channel catfish

    International Nuclear Information System (INIS)

    A feeding experiment conducted in a controlled environment and using a vitamin B12-deficient, but otherwise nutritionally complete, purified diet revealed that intestinal microorganisms in channel catfish synthesized approximately 1.4 ng of vitamin B12 per gram of bodyweight per day. Removal of cobalt from the diet or supplementation with an antibiotic (succinylsulfathiazole) significantly reduced the rate of intestinal synthesis and liver stores of vitamin B12. Radiolabeled vitamin B12 in the blood, liver, kidneys, and spleen of fish fed 60Co in the diet indicated that the intestinally synthesized vitamin was absorbed by the fish. The primary route of absorption was directly from the digestive tract into the blood because coprophagy was prevented in the rearing aquariums and the amount of vitamin B12 dissolved in the aquarium water was too low for gill absorption. Dietary supplementation of vitamin B12 was not necessary for normal growth and erythrocyte formation in channel catfish in a 24-week feeding period. A longer period, however, may have caused a vitamin deficiency since liver-stored vitamin B 12 decreased between the 2nd and 24th weeks

  18. Analysis of matrix proteins of otolith in upside-down catfish

    Science.gov (United States)

    Ohnishi, K.; Okamoto, N.; Takahashi, A.; Ohnishi, T.

    We have previously suggested that the calcium density of the otolith in upside-down swimming Synodontis nigriventris is lower than that in upside-up swimming Synodontis multipunctatus Biol Space Sci 2002 In this study we examined EDTA-soluble matrix proteins of otolith in the utricle of the catfish S nigriventris S multipunctatus and upside-up swimming Synodontis brichadi and goldfish Carassius auratus We detected two main bands about 55 kD and 80 kD with SDS-PAGE in the 3 species of the catfish In cntrast goldfish had the about 55 kD band alone The band of about 80 kD was consisted of two sub-bands a lighter and a heavier band A lighter band was observed in S brichadi and a heavier band was observed in S nigriventris S multipunctatus had the both bands Furthermore mass spectrometric analysis showed there were some proteins of molecular weight under 14 kD The molecular weights of the proteins were different among the fishes These results suggest that many different kinds of matrix protein may cause different degree of calcification in otolith formation

  19. Preliminary study on element mass fraction determination on catfish samples from Paraguay

    International Nuclear Information System (INIS)

    South American catfish (Pseudoplatystoma), commonly known in Spanish as atigrado or surubi and in Portuguese as surubim or pintado is a large fish that typically reaches 1 m long and weighs 60 kg to 80 kg and may be found at the basins of the Amazon, the Sao Francisco and de la Plata rivers, usually in riverbeds and deep wells. Being a much appreciated fish for human consumption, it is quite sought after by fishermen who have been contributing to the reduction of the stocks. This fact attracted the attention of the Paraguayan authorities to the point of imposing restrictions to free fishing and commercialization. This study aims to assist the conservation efforts towards this fish by investigating its exposure to possible pollutants. Preliminary results on element determination on six samples of catfish from Paraguayan rivers are presented. Cs, Co, Fe, Se and Zn were determined by applying an Instrumental Neutron Activation Analysis method. While these element levels were lower than the legislation for human consumption, the elements As, Cr e La were not detected in the samples as they are below the detection limit of the method employed. Atomic Absorption Spectrometry was used to investigate the presence of Cd, Hg and Pb in the samples. Hg was detected in the samples while Cd and Pb were below the detection limit of the method. (author)

  20. Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge.

    Science.gov (United States)

    Zhang, Dunhua; Moreira, Gabriel S A; Shoemaker, Craig; Newton, Joseph C; Xu, De-Hai

    2016-05-01

    The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a time course was detected and quantified using real-time polymerase chain reaction. The results showed that 77.8% of fish died within 48 h post challenge with mean day to death of 1.5 days. At 2 h post challenge, vAh (inferred from genomic DNA copies or genome equivalents) was detected in all external and internal tissues sampled. Gill had the highest vAh cells at 1 h post challenge. Spleen harbored the most vAh cells among internal organs at 4 h post challenge. The tissues/organs with most vAh cells detected at 8 h post challenge were adipose fin, blood, intestine, kidney and skin, while liver showed the highest vAh cells at 24 h post challenge. These results suggest that vAh was able to rapidly proliferate and spread, following wound infection, through the fish blood circulation system and cause mortality within 8-24 h. PMID:27044300

  1. The leachability, bioaccessibility, and speciation of Cu in the sediment of channel catfish ponds

    International Nuclear Information System (INIS)

    There have been growing concerns about the environmental impact of Cu applied in the catfish pond aquaculture. In this paper, sediments taken from three commercial catfish ponds were studied for content, leachability, bioaccessibility, and speciation of sediment-bound Cu. Results showed that the Cu was concentrated in the top 10 cm of the sediments and the peak Cu concentrations ranged from the background level to about 200 mg/kg. Toxicity characteristic leaching procedure showed only 1-8% of sediment Cu was leachable while bioaccessible Cu, evaluated by physiological based extraction test, accounted for up to 40-85% of total Cu. Due to the high redox potential in the surface sediments, acid-volatile sulfide was not a significant binding phase. The sequential extraction results showed that the residual phase (forms in lattices of primary and secondary minerals) was the major Cu fraction in the first two pond sediments but carbonate-bound, Fe/Mn oxide-bound and organically bound Cu, as well as the residual fraction, seemed equally important in the third pond. - Careful disposal of the Cu-laden pond sediment is necessary

  2. Optimization of Supercritical CO2 Extraction of Fish Oil from Viscera of African Catfish (Clarias gariepinus

    Directory of Open Access Journals (Sweden)

    Mohamed Zaidul Islam Sarker

    2012-09-01

    Full Text Available Fish oil was extracted from the viscera of African Catfish using supercritical carbon dioxide (SC-CO2. A Central Composite Design of Response Surface methodology (RSM was employed to optimize the SC-CO2 extraction parameters. The oil yield (Y as response variable was executed against the four independent variables, namely pressure, temperature, flow rate and soaking time. The oil yield varied with the linear, quadratic and interaction of pressure, temperature, flow rate and soaking time. Optimum points were observed within the variables of temperature from 35 °C to 80 °C, pressure from 10 MPa to 40 MPa, flow rate from 1 mL/min to 3 mL/min and soaking time from 1 h to 4 h. However, the extraction parameters were found to be optimized at temperature 57.5 °C, pressure 40 MPa, flow rate 2.0 mL/min and soaking time 2.5 h. At this optimized condition, the highest oil yields were found to be 67.0% (g oil/100 g sample on dry basis in the viscera of catfish which was reasonable to the yields of 78.0% extracted using the Soxhlet method.

  3. Preliminary study on element mass fraction determination on catfish samples from Paraguay

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Edson G.; Catharino, Marilia G.M.; Vasconcellos, Marina B.A., E-mail: emoreira@ipen.br, E-mail: mbvascon@ipen.br, E-mail: mariliasemmler@uol.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Frutos, Sixto A.; Insaurralde, Mario S., E-mail: tony8013@hotmail.com, E-mail: insaurraldemar9@hotmail.com [Universidad Nacional de Asuncion (FCV/UNA), San Lorenzo (Paraguay). Facultad de Ciencias Veterinarias. Departamento de Pesca y Acuicultura

    2013-07-01

    South American catfish (Pseudoplatystoma), commonly known in Spanish as atigrado or surubi and in Portuguese as surubim or pintado is a large fish that typically reaches 1 m long and weighs 60 kg to 80 kg and may be found at the basins of the Amazon, the Sao Francisco and de la Plata rivers, usually in riverbeds and deep wells. Being a much appreciated fish for human consumption, it is quite sought after by fishermen who have been contributing to the reduction of the stocks. This fact attracted the attention of the Paraguayan authorities to the point of imposing restrictions to free fishing and commercialization. This study aims to assist the conservation efforts towards this fish by investigating its exposure to possible pollutants. Preliminary results on element determination on six samples of catfish from Paraguayan rivers are presented. Cs, Co, Fe, Se and Zn were determined by applying an Instrumental Neutron Activation Analysis method. While these element levels were lower than the legislation for human consumption, the elements As, Cr e La were not detected in the samples as they are below the detection limit of the method employed. Atomic Absorption Spectrometry was used to investigate the presence of Cd, Hg and Pb in the samples. Hg was detected in the samples while Cd and Pb were below the detection limit of the method. (author)

  4. Studies on the cranial osteology of the blind catfish Horoglanis krishnai Menon (Pisces, Clariidae.

    Directory of Open Access Journals (Sweden)

    Mercy T.V. Anna

    2001-01-01

    Full Text Available Horaglanis krishnai Menon is a blind catfish inhabiting the dug- out wells at Kottayam, Kerala, South India. Studies on the cranial osteology of the fish show that the bones on the skull are firmly articulated. The frontoparietal fontanella is very large so that the cranium virtually lacks a roof. The sphenotics and alisphenoids are hardly recognizable and the orbital bones are entirely lacking. In osteological features H. krishnai closely resembles Uegitglanis zammaroni. But in H. krishnai the orbital bones are further reduced or even absent. The fontanella is larger than that of any other known catfish. These two species must have evolved from the same ancester and have taken up nearly identical ways of life. The difference between the skeletons of these two appears to be largely dependent on the relative size of the frontoparietal fontanella. Its greater development in H. krishnai brought about a suppression or reduction of some of the bones clearly visible in Uegitglanis. It would appear that the modification initiated in Uegitglanis gatered momentum in Horaglanis. These two fishes form a group distinct from clariids and bagrids but form a connecting link between the two.

  5. Distribution of catfishes in wetlands of two flood plain districts in Tamil Nadu, India

    Directory of Open Access Journals (Sweden)

    B. Rajagopal

    2013-12-01

    Full Text Available A study was conducted on the distribution of catfishes in selected wetlands in Kancheepuram and Kanyakumari districts of Tamil Nadu, southern India. Different types of wetlands such as tanks, pools, lakes, open wells and estuaries were selected for the study based on their different environmental set up. Fishes were collected with the help of fishermen using cast and seine nets. Twelve species of catfishes from five families (Ariidae, Bagridae, Heteropneustidae, Schilbeidae and Siluridae were recorded, of which 10 species from four families were from Kanyakumari and six species belonging to three families were from Kancheepuram District. In Kancheepuram, the species recorded were Heteropneustes fossilis, Mystus seengtee, M. gulio, M. keletius, M. vittatus and Neotropius atherinoides, and in Kanyakumari the species recorded were Arius arius, Arius subrostratus, Heteropneustes fossilis, Mystus armatus, M. seengtee, M. gulio, M. montanus, M. vittatus, Ompok bimaculatus and O. malabaricus. Among the wetlands, the highest species richness was seen in Puthery and Erachakulam tanks in Kanyakumari and Chembarampakkam Lake in Kancheepuram. The lowest species richness was observed was in Vishnupuram, Thotiode tanks and Mavadi pool of the former district and Vandalur Tank, Kalpakkam Estuary of the latter. Environmental factors such as microhabitat diversity and substrate diversity in the wetlands significantly influenced species richness.

  6. Malathion exposure induces the endocrine disruption and growth retardation in the catfish, Clarias batrachus (Linn.).

    Science.gov (United States)

    Lal, Bechan; Sarang, Mukesh Kumar; Kumar, Pankaj

    2013-01-15

    Many hormones are known for their role in the regulation of metabolic activities and somatic growth in fishes. The present study deals with the effects of malathion (an organophosphorous pesticide) on the levels of metabolic hormones that are responsible for promotion of somatic and ovarian growth of the freshwater catfish, Clarias batrachus. Malathion treatment for thirty days drastically reduced the food intake and body weight of fish. These fish also exhibited a great avoidance to food. Exposure of catfish to malathion reduced the levels of thyroxine (T(4)), triiodothyronine (T(3)), growth hormone (GH), insulin like growth factor-I (IGF-I), testosterone (T) and estradiol-17β (E(2)) in a dose dependent manner during all the studied reproductive phases, in general, except that malathion increased the level of GH during the quiescence phase. Significant reduction in muscle and hepatic protein content also occurred in the malathion-treated fish. Malathion exposure induced lipolysis too in the liver and muscle. The results thus support that malathion treatment disrupts the endocrine functions and the olfactory sensation responsible for food intake and gustatory feeding behavior, which ultimately leads to retardation of fish growth. PMID:23174696

  7. Development of the lateral line mechanoreceptors in the catfish Silurus glanis

    Science.gov (United States)

    Roth, Anton

    2010-07-01

    In fish the neuromasts of the lateral line develop as follows: a “migrating primordium” (MP) migrates from the postotic placode along the future posterior lateral line canal to the tail fin. Its placodal cells are assumed to be the basis for the development of the neuromasts. However, an MP was not found in all fish, e.g., in catfish. In the present study, a search was made for the MP in the larvae of the catfish Silurus glanis. Using light and electron microscopy, an MP was found to migrate along the ventral rim of the tail. It precedes the formation of a ventral row of free neuromasts. An MP preceding the main lateral row of the future canal neuromasts was not found. The necessity of the MP for the ventral-free neuromast development is shown by making incisions which block its migration. The result: caudal to the incision site neuromasts do not develop. On the other hand, the same incision procedure applied to the assumed migration route of a—hypothetical—lateral MP does not block the development of the lateral neuromasts. It is concluded that in this case an MP is not necessary for the development of the canal neuromasts.

  8. Embryonic and larval development of critically endangered riverine catfish Rita rita

    Directory of Open Access Journals (Sweden)

    Muhammad Fazlul Awal Mollah

    2011-11-01

    Full Text Available The present study was carried out to investigate the embryonic and larval development of freshwater catfish Rita rita. The mature eggs and sperms were collected by using artificial insemination technique and fertilized eggs were incubated in mini circular hatchery with provision of continuously water supply. The fertilized eggs were transparent, demarsal, spherical, non-adhesive and brownish in colour with a diameter ranging between 1.3 to 1.6 mm. First cleavage occurred within 25-30 min post-fertilization at temperature of 28±1°C. Hatching started 22 h post-fertilization and completed within 24 h at the same temperature range. Newly hatched larvae were 2.0 mm in length devoid of mouth and pigmentation and started feeding at 48 to 60 h post-hatching. To date, this is the first time the early embryonic and larval development of freshwater catfish R. rita is described. Thus the findings of the present study provide valuable information that may help establishing the large scale seed production technique of Rita.

  9. Inhibition of nitrite-induced toxicity in channel catfish by calcium chloride and sodium chloride

    Science.gov (United States)

    Tommasso J.R., Wright; Simco, B.A.; Davis, K.B.

    1980-01-01

    Environmental chloride has been shown to inhibit methemoglobin formation in fish, thereby offering a protective effect against nitrite toxicity. Channel catfish (Ictalurus punctatus) were simultaneously exposed to various environmental nitrite and chloride levels (as either CaCl2 or NaCl) in dechlorinated tap water (40 mg/L total hardness, 47 mg/L alkalinity, 4 mg/L chloride, pH = 6.9-7.1, and temperature 21-24°C). Methemoglobin levels in fish simultaneously exposed to 2.5 mg/L nitrite and up to 30 mg/L chloride as either CaCl2 or NaCl were similar but significantly lower than in unprotected fish. Exposure to 10 mg/L nitrite and 60 mg/L chloride resulted in methemoglobin levels similar to those of the controls; most unprotected fish died. Fish exposed to 10 mg/L nitrite had significantly lower methemoglobin levels when protected with 15.0 mg/L chloride as CaCl2 than with NaCl. Fish exposed to nitrite in the presence of 60 mg/L chloride (as either CaCl2 or NaCl) had similar 24-h LC50 values that were significantly elevated above those obtained in the absence of chloride. Calcium had little effect on tolerance to nitrite toxicity in channel catfish in contrast to its large effect reported in steelhead trout (Salmo gairdneri).

  10. Genetic structure of populations and conservation issues relating to an endangered catfish, Clarias batrachus, in India.

    Science.gov (United States)

    Khedkar, Gulab D; Tiknaik, Anita; Kalyankar, Amol D; A, Chandra Sekhar Reddy; Khedkar, Chandraprakash D; Ron, Tetsuzan Benny; Haymer, David

    2016-01-01

    The Asian catfish, Clarias batrachus (Linnaeus, 1758), is a highly valued species endemic to India that is currently in drastic decline in most of its natural habitat. The present study was undertaken to document the genetic structure of populations of this species using mitochondrial DNA markers, specifically from the cytochrome B and D-loop regions. Specimens from eight wild populations were collected and analyzed from different regions in India. The genetic variation within and among populations was evaluated using a range of descriptive statistics. The analysis described here provides a broad and consistent view of population structure and demographic history of populations of C. batrachus. Although there was some genetic structuring consistent with regional differences, all eight populations examined here showed relatively low levels of genetic variation in terms of both haplotype and nucleotide diversities in the different analyses used. However, a number of private haplotypes were discovered, and this may provide valuable information for future selective breeding program and conservation management. The results may aid in the design and implementation of strategies for the future management of this endangered catfish C. batrachus in India.

  11. Observation and hydrodynamic analysis of fast-start of yellow catfish (Pelteobagrus fulvidraco

    Institute of Scientific and Technical Information of China (English)

    JING Jun; YIN Xiezhen; LU Xiyun

    2005-01-01

    The kinematics and hydrodynamics of the escape response of yellow catfish ( Pelteobagrus fulvidraco ) is investigated.It is observed from digital high-speed camera that all the escape response of yellow catfish is a C-type fast-start, which is characterized by the C-shape of the fish body at the end of the first contraction of the lateral musculature. Based on the analysis of the mass center movement and the performance of the caudal fin, the fast-start process can be divided into two stages: in stage one the fish body rotates rapidly around its mass center and in stage two the fish begins moving straightly. Theoretically, the Weihs' model is employed to obtain the forces, moments and turning angular accelerations acting on the fish during the escape response. The results are in good agreement with the experimental observation. Furthermore, muscle strain at different locations along the body is calculated. At last, the dynamical performance of the escape response is explained.

  12. Elimination of 137Cs from Japanese Catfish Acutely Contaminated by Labelled Food

    International Nuclear Information System (INIS)

    The effective half-life of 137Cs in acutely contaminated Japanese catfish was determined. The fish were fed with fish meal incorporated with 137CsCl and then released into aquaria. To assess the 137Cs activity retained in the fish body, the whole body radioactivity of each fish was measured at regular intervals for up to 157 days. The data were plotted with relative counts on the y-axis and corresponding elapsed time on the x-axis. Relative count plotted against elapsed time was found to consist of three components, namely the first fast, second fast and the slow component. The true elimination curves of the first components were calculated by the peeling off method. The effective half-life of the fast components was determined from the slope of the true curves. The effective half-life of 137Cs for the first fast, second fast and the slow component in Japanese catfish was found to be 1.27, 5.76 and 251 days, respectively. (author)

  13. Genetic structure of populations and conservation issues relating to an endangered catfish, Clarias batrachus, in India.

    Science.gov (United States)

    Khedkar, Gulab D; Tiknaik, Anita; Kalyankar, Amol D; A, Chandra Sekhar Reddy; Khedkar, Chandraprakash D; Ron, Tetsuzan Benny; Haymer, David

    2016-01-01

    The Asian catfish, Clarias batrachus (Linnaeus, 1758), is a highly valued species endemic to India that is currently in drastic decline in most of its natural habitat. The present study was undertaken to document the genetic structure of populations of this species using mitochondrial DNA markers, specifically from the cytochrome B and D-loop regions. Specimens from eight wild populations were collected and analyzed from different regions in India. The genetic variation within and among populations was evaluated using a range of descriptive statistics. The analysis described here provides a broad and consistent view of population structure and demographic history of populations of C. batrachus. Although there was some genetic structuring consistent with regional differences, all eight populations examined here showed relatively low levels of genetic variation in terms of both haplotype and nucleotide diversities in the different analyses used. However, a number of private haplotypes were discovered, and this may provide valuable information for future selective breeding program and conservation management. The results may aid in the design and implementation of strategies for the future management of this endangered catfish C. batrachus in India. PMID:25103426

  14. Spontaneous behavior of basal Copionodontinae cave catfishes from Brazil (Teleostei, Siluriformes, Trichomycteridae

    Directory of Open Access Journals (Sweden)

    Bianca Rantin

    2015-08-01

    Full Text Available Cave animals are particularly interesting due to their behavioral specializations, resultant from evolution in isolation. We present data from a spontaneous behavior study (spatial distribution and preference for microhabitats of two troglobitic catfish from Brazil: Glaphyropoma spinosum and a new species of Copionodon. We compared the data with those obtained of a sympatric epigean species, Copionodon pecten. These Trichomycteridae species belong to a basal and apparently monophyletic subfamily – Copionodontinae, endemic to Chapada Diamantina, central Bahia state, eastern Brazil. We observed the fishes in natural and laboratory conditions through ad libitum and focal animal methods. Each spatial behavioral category (hidden, bottom, midwater, surface and wall swimming and stationary in the bottom was timed individually, with a sample of 12 specimens per species. Unlike most troglobitic fishes, cave copionodontines tested herein did not extend exploratory behavior to midwater, with benthonic and thigmotactic-related exploratory behavior. This behavior is possibly related to its feeding behavior specializations, strong territorialism and photophobic behavior. The epigean Copionodon species is also benthonic. The spatial behavior of the cave Copionodontinae could be interpreted as a retained and plesiomorphic character-state in relation to other trichomycterid catfishes.

  15. Using 1-D and 2-D J-resolved 1H-NMR metabolomics to understand the effects of anemia in channel catfish Ictalurus punctatus

    Science.gov (United States)

    Anemia is a widespread hematological disorder in vertebrates. In channel catfish (Ictalurus punctatus), anemia is a persistent problem in culture environments, however, its causes and physiological impacts are not well understood. To better understand the symptoms and characterize associated biomark...

  16. Using 1-D 1H and 2-D 1H J-resolved NMR metabolomics to understand the effects of anemia in channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    Anemia is a widespread hematological disorder in vertebrates. In channel catfish (Ictalurus punctatus), anemia is a persistent problem in culture environments, however, its causes and physiological impacts are not well understood. To better understand the symptoms and characterize associated biomark...

  17. In vitro and in vivo evaluation of potassium permanganate treatment efficacy for the control of acute experimental infection of flavobacterium columnare in channel catfish

    Science.gov (United States)

    An experimental trial was performed to evaluate the efficacy of potassium permanganate against an acute and systemic experimental infection of Flavobacterium columnare in channel catfish, Ictalurus punctatus. The infection was produced by waterborne exposure to the bacteria after mechanical cutaneo...

  18. What are the Economic Prospects of Developing Aquaculture in Queensland to Supply the Low Price White Fillet Market? Lessons from the US Channel Catfish Industry

    OpenAIRE

    Lyster, Thorbjorn

    2004-01-01

    The farming of channel catfish (Ictalurus punctatus) is the largest (by volume and value) and most successful (in terms of market impact) aquaculture industry in the United States of America. Farmed channel catfish is the most consumed (in terms of volume per capita) fish fillet in the U.S. market. Within Australia, it has long been suggested by researchers and industry that silver perch (Bidyanus bidyanus) and possibly other endemic teraponid species possess similar biological attributes for...

  19. Diverse Early Life-History Strategies in Migratory Amazonian Catfish: Implications for Conservation and Management.

    Directory of Open Access Journals (Sweden)

    Jens C Hegg

    Full Text Available Animal migrations provide important ecological functions and can allow for increased biodiversity through habitat and niche diversification. However, aquatic migrations in general, and those of the world's largest fish in particular, are imperiled worldwide and are often poorly understood. Several species of large Amazonian catfish carry out some of the longest freshwater fish migrations in the world, travelling from the Amazon River estuary to the Andes foothills. These species are important apex predators in the main stem rivers of the Amazon Basin and make up the region's largest fishery. They are also the only species to utilize the entire Amazon Basin to complete their life cycle. Studies indicate both that the fisheries may be declining due to overfishing, and that the proposed and completed dams in their upstream range threaten spawning migrations. Despite this, surprisingly little is known about the details of these species' migrations, or their life history. Otolith microchemistry has been an effective method for quantifying and reconstructing fish migrations worldwide across multiple spatial scales and may provide a powerful tool to understand the movements of Amazonian migratory catfish. Our objective was to describe the migratory behaviors of the three most populous and commercially important migratory catfish species, Dourada (Brachyplatystoma rousseauxii, Piramutaba (Brachyplatystoma vaillantii, and Piraíba (Brachyplatystoma filamentosum. We collected fish from the mouth of the Amazon River and the Central Amazon and used strontium isotope signatures ((87Sr/(86Sr recorded in their otoliths to determine the location of early rearing and subsequent. Fish location was determined through discriminant function classification, using water chemistry data from the literature as a training set. Where water chemistry data was unavailable, we successfully in predicted (87Sr/(86Sr isotope values using a regression-based approach that related

  20. Effect of nutrient restriction and re-feeding on calpain family genes in skeletal muscle of channel catfish (Ictalurus punctatus.

    Directory of Open Access Journals (Sweden)

    Elena Preziosa

    Full Text Available BACKGROUND: Calpains, a superfamily of intracellular calcium-dependent cysteine proteases, are involved in the cytoskeletal remodeling and wasting of skeletal muscle. Calpains are generated as inactive proenzymes which are activated by N-terminal autolysis induced by calcium-ions. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we characterized the full-length cDNA sequences of three calpain genes, clpn1, clpn2, and clpn3 in channel catfish, and assessed the effect of nutrient restriction and subsequent re-feeding on the expression of these genes in skeletal muscle. The clpn1 cDNA sequence encodes a protein of 704 amino acids, Clpn2 of 696 amino acids, and Clpn3 of 741 amino acids. Phylogenetic analysis of deduced amino acid sequences indicate that catfish Clpn1 and Clpn2 share a sequence similarity of 61%; catfish Clpn1 and Clpn3 of 48%, and Clpn2 and Clpn3 of only 45%. The domain structure architectures of all three calpain genes in channel catfish are similar to those of other vertebrates, further supported by strong bootstrap values during phylogenetic analyses. Starvation of channel catfish (average weight, 15-20 g for 35 days influenced the expression of clpn1 (2.3-fold decrease, P<0.05, clpn2 (1.3-fold increase, P<0.05, and clpn3 (13.0-fold decrease, P<0.05, whereas the subsequent refeeding did not change the expression of these genes as measured by quantitative real-time PCR analysis. Calpain catalytic activity in channel catfish skeletal muscle showed significant differences only during the starvation period, with a 1.2- and 1.4- fold increase (P<0.01 after 17 and 35 days of starvation, respectively. CONCLUSION/SIGNIFICANCE: We have assessed that fasting and refeeding may provide a suitable experimental model to provide us insight into the role of calpains during fish muscle atrophy and how they respond to changes in nutrient supply.

  1. Kinetics of intracardially injected plutonium-237 citrate in channel catfish (Ictalurus punctatus)

    International Nuclear Information System (INIS)

    Plutonium-237 distribution and translocation in channel catfish following intracardial injection are basically similar to patterns in mammals after intravenous administration. Bone and liver are the dominant contributors to the body burden. The fractional body burdens in bone, liver and kidney 17 days after injection were 31, 24 and 9% of the injected dose, respectively. High kidney burdens relative to mammals are expected since the kidney functions as the major site of hemopoeisis in teleosts. The observation of high concentrations in some ovaries, coupled with apparent polymorphism in plutonium-binding plasma proteins, suggests that some aspects of plutonium transport in blood plasma, of lower vertebrates may differ significantly from the mammals. Excretion over the 17-day period was <= 10%. The absence of significant excretion indicates that a short half-life component of elimination following gut clearance in earlier gavage studies is due to plutonium labelling of the gut. (author)

  2. De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome

    Science.gov (United States)

    Schulze, Thomas T.; Ali, Jonathan M.; Bartlett, Maggie L.; McFarland, Madalyn M.; Clement, Emalie J.; Won, Harim I.; Sanford, Austin G.; Monzingo, Elyssa B.; Martens, Matthew C.; Hemsley, Ryan M.; Kumar, Sidharta; Gouin, Nicolas; Kolok, Alan S.; Davis, Paul H.

    2016-01-01

    Trichomycterus areolatus is an endemic species of pencil catfish that inhabits the riffles and rapids of many freshwater ecosystems of Chile. Despite its unique adaptation to Chile's high gradient watersheds and therefore potential application in the investigation of ecosystem integrity and environmental contamination, relatively little is known regarding the molecular biology of this environmental sentinel. Here, we detail the assembly of the Trichomycterus areolatus transcriptome, a molecular resource for the study of this organism and its molecular response to the environment. RNA-Seq reads were obtained by next-generation sequencing with an Illumina® platform and processed using PRINSEQ. The transcriptome assembly was performed using TRINITY assembler. Transcriptome validation was performed by functional characterization with KOG, KEGG, and GO analyses. Additionally, differential expression analysis highlights sex-specific expression patterns, and a list of endocrine and oxidative stress related transcripts are included. PMID:27672404

  3. Specificity of the collagenolytic serine proteinase from the pancreas of the catfish (Parasilurus asotus).

    Science.gov (United States)

    Yoshinaka, R; Sato, M; Yamashita, M; Itoko, M; Ikeda, S

    1987-01-01

    The collagenolytic serine proteinase from the pancreas of the catfish (Parasilus asotus) had a pH optimum of 7.5 for native, reconstituted calf skin collagen fibrils. The enzyme was most stable at pH 6-9. The enzyme hydrolyzed heat-denatured collagen (gelatin), casein, hemoglobin and elastin in addition to native collagen but not virtually Tos-Arg-OEe, Bz-Tyr-OEe and Suc-(Ala)3-NA. The enzyme cleaved Leu-Gly (or Gln-Gly), Gly-Ile and Ile-Ala bonds on DNP-Pro-Leu-Gly-Ile-Ala-Gly-Arg-NH2 and DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg. PMID:3480788

  4. Liquid-chromatographic determination of sarafloxacin residues in channel catfish muscle-tissue

    Science.gov (United States)

    Meinertz, J.R.; Dawson, V.K.; Gingerich, W.H.; Cheng, B.; Tubergen, M.M.

    1994-01-01

    A liquid chromatographic method is described for the determination of sarafloxacin hydrochloride residues i n channel catfish (ictalurus punctatus) fillets. Sarafloxacin was extracted from fillet tissue with acetonitrile=water (1 + 1). The extract was centrifuged and the supernatant was partitioned with hexane. The aqueous fraction was filtered through a 0.45 Mum filter and evaporated to dryness. The sample was redissolved with 20% acetonitrile-methanol (3 + 2) and 80% trifluoroacetic acid (0.1%), Centrifuged, and filtered to remove proteins. Samples were analyzed by chromatography with gradient elution on a c18 column and with fluorescence detection (excitation at 280 nm and emission above 389 nm). Mean recoveries ranged from 85.4 To 104%, and relative standard deviations ranged from 1.06 To 5.58% In samples spiked at concentrations of 10.0-863.8 Ng/g. The method detection limit for sarafloxacin was 1.4 Ng/g.

  5. Genetic characterization of the Neotropical catfish Pimelodus maculatus (Pimelodidae, Siluriformes) in the Upper Uruguay River

    Science.gov (United States)

    Ribolli, Josiane; de Melo, Cláudio Manoel Rodrigues; Zaniboni-Filho, Evoy

    2012-01-01

    Freshwater fish present unique challenges when one attempts to understand the factors that determine the structure of their populations. Habitat fragmentation is a leading cause of population decline that threatens ecosystems worldwide. In this study, we investigated the conservation status of genetic variability in the Neotropical catfish (Pimelodus maculatus). Specifically, we examined the structure and genetic diversity of this species in a region of the Upper Uruguay River fragmented by natural barriers and dams. There was no genetic structure among the four sites analyzed, indicating the existence of only one population group. A combination of environmental management and genetic monitoring should be used to minimize the impact of impoundment on panmitic populations of migratory fish species. PMID:23271936

  6. The effect of environmental hypercapnia and size on nitrite toxicity in the striped catfish (Pangasianodon hypophthalmus)

    DEFF Research Database (Denmark)

    Hvas, Malthe; Damsgaard, Christian; Gam, Le Thi Hong;

    2016-01-01

    Striped catfish (Pangasianodon hypophthalmus) are farmed intensively at high stocking densities in Vietnam where they are likely to encounter environmental hypercapnia as well as occasional high levels of aquatic nitrite. Nitrite competes with Cl- for uptake at the branchial HCO3-/Cl- exchanger...... to a reduced nitrite uptake. To assess the effect of hypercapnia on nitrite uptake, fish were cannulated in the dorsal aorta, allowing repeated blood sampling for measurements of haemoglobin derivatives, plasma ions and acid-base status during exposure to 0.9 mM nitrite alone and in combination with...... acute and 48 h acclimated hypercapnia over a period of 72 h. Nitrite uptake was initially reduced during the hypercapnia-induced acidosis, but after pH recovery the situation was reversed, resulting in higher plasma nitrite concentrations and lower functional haemoglobin levels that eventually caused...

  7. Establishment and characterization of an ovarian cell line from Southern catfish (Silurus meridionalis).

    Science.gov (United States)

    Wei, Jing; Qi, WenChuang; Zhou, Yujie; Zhang, Xiaoping; Dong, Ranran; Zhou, Linyan; Wang, Deshou

    2014-10-01

    An ovarian cell line was successfully developed from the juvenile ovary of Southern catfish (SCO1) (Silurus meridionalis), which was designated as SCO1. The cell line multiplied preferentially in L-15 medium with 15 % fetal bovine serum at 28 °C for more than 70 passages over a period of 420 days. SCO1 showed fibroblast-like morphology and predominantly retained a diploid karyotype of 58 chromosomes. From the gene expression patterns, SCO1 showed a characteristic of ovarian granulosa cells. After the cells were transfected with the green fluorescent protein expression vector, bright fluorescent signals could be observed in approximately 30 % cells. This cell line may be valuable for the evaluation of endocrine disruptors and studying interactions between somatic cells and germ cells. PMID:24671650

  8. Pentachlorophenol-Induced Cytotoxic, Mitogenic, and Endocrine-Disrupting Activities in Channel Catfish, Ictalurus punctatus

    Directory of Open Access Journals (Sweden)

    Paul B. Tchounwou

    2004-09-01

    Full Text Available Pentachlorophenol (PCP is an organochlorine compound that has been widely used as a biocide in several industrial, agricultural, and domestic applications. Although it has been shown to induce systemic toxicity and carcinogenesis in several experimental studies, the literature is scarce regarding its toxic mechanisms of action at the cellular and molecular levels. Recent investigations in our laboratory have shown that PCP induces cytotoxicity and transcriptionally activates stress genes in human liver carcinoma (HepG2 cells [1]. In this research, we hypothesize that environmental exposure to PCP may trigger cytotoxic, mitogenic, and endocrine-disrupting activities in aquatic organisms including fish. To test this hypothesis, we carried out in vitro cultures of male channel catfish hepatocytes, and performed the fluorescein diacetate assay (FDA to assess for cell viability, and the Western Blot analysis to assess for vitellogenin expression following exposure to PCP. Data obtained from FDA experiments indicated a strong dose-response relationship with respect to PCP cytotoxicity. Upon 48 hrs of exposure, the chemical dose required to cause 50% reduction in cell viability (LD50 was computed to be 1,987.0 + 9.6 μg PCP/mL. The NOAEL and LOAEL were 62.5 + 10.3 μg PCP/mL and 125.0+15.2 μg PCP/mL, respectively. At lower levels of exposure, PCP was found to be mitogenic, showing a strong dose- and time-dependent response with regard to cell proliferation. Western Blot analysis demonstrated the potential of PCP to cause endocrine-disrupting activity, as evidenced by the up regulation of the 125-kDa vitellogenin protein the hepatocytes of male channel catfish.

  9. Molecular immune response of channel catfish immunized with live theronts of Ichthyophthirius multifiliis.

    Science.gov (United States)

    Xu, De-Hai; Zhang, Qi-Zhong; Shoemaker, Craig A; Zhang, Dunhua; Moreira, Gabriel S A

    2016-07-01

    The parasite Ichthyophthirius multifiliis (Ich) has been reported in various freshwater fishes worldwide and results in severe losses to both food and aquarium fish production. The fish surviving natural infections or immunized with live theronts develop strong specific and non-specific immune responses. Little is known about how these immune genes are induced or how they interact and lead to specific immunity against Ichthyophthirius multifiliis in channel catfish Ictalurus punctatus. This study evaluated the differential expression of immune-related genes, including immunoglobulin, immune cell receptor, cytokine, complement factor and toll-like receptors in head kidney from channel catfish at different time points after immunization with live theronts of I. multifiliis. The immunized fish showed significantly higher anti-Ich antibody expressed as immobilization titer and ELISA titer than those of control fish. The vast majority of immunized fish (95%) survived theront challenge. Expression of IgM and IgD heavy chain genes exhibited a rapid increase from 4 hour (h4) to 2 days (d2) post immunization. Expression of immune cell receptor genes (CD4, CD8-α, MHC I, MHC II β, TcR-α, and TcR-β) showed up-regulation from h4 to d6 post immunization, indicating that different immune cells were actively involved in cellular immune response. Cytokine gene expression (IL-1βa, IL-1βb, IFN-γ and TNF-α) increased rapidly at h4 post immunization and were at an up-regulated level until d2 compared to the bovine serum albumin control. Expression of complement factor and toll-like receptor genes exhibited a rapid increase from h4 to d2 post immunization. Results of this study demonstrated differential expression of genes involved in the specific or non-specific immune response post immunization and that the vaccination against Ich resulted in protection against infection by I. multifiliis. PMID:27044331

  10. Skin of the male African catfish, Clarias gariepinus: a source of steroid glucuronides

    International Nuclear Information System (INIS)

    Steroid metabolism in the skin of mature male African catfish, Clarias gariepinus, reared in the laboratory, was studied in vitro by tissue incubations with [3H]pregnenolone, [3H]dehydroepiandrosterone, [3H]17 alpha-hydroxyprogesterone, [3H]androstenedione, [14C]11 beta-hydroxyandrostenedione, and [3H]testosterone as precursors. While pregnenolone was not converted to any other steroid, dehydroepiandrosterone was transformed mainly to 5-androstene-3 beta, 17 beta-diol. The products of 17 alpha-hydroxyprogesterone incubations were 5 beta-pregnane-3 alpha,17 alpha-diol-20-one, 5 beta-pregnane-3 alpha,17 alpha, 20 beta-triol, and 5 beta-pregnan-17 alpha-o1-3,20-dione. The major steroids of androstenedione incubations were etiocholanolone, testosterone, and androsterone. Testosterone was converted mainly to etiocholanolone and androstenedione, and only small quantities of 11 beta-hydroxytestosterone, 11-ketotestosterone, and 11-ketoandrostenedione were the metabolites found in 11 beta-hydroxyandrostenedione incubation. These results demonstrated the presence of the enzymes 5 alpha- and 5 beta-reductases and 3 alpha-, 11 beta-, 17 beta-, and 20 beta-hydroxysteroid dehydrogenases in the skin. From enzymehistochemical results it appeared that the steroid conversions take place in the epithelial cells. Moreover, the presence of UDP-glucose dehydrogenase, an enzyme involved in the synthesis of glucuronic acid, in these cells indicates the possibility of steroid glucuronide formation. Indeed significant amounts of water-soluble steroid conjugates, particularly 5 beta-dihydrotestosterone- and testosterone-glucuronide, were found in the incubations with androstenedione and testosterone, indicating the presence of the UDP-glucuronosyl transferase in the catfish skin

  11. Patterns of 137Cs accumulation in bullhead catfish inhabiting an abandoned reactor reservoir

    International Nuclear Information System (INIS)

    The author assayed muscle 137Cs concentrations of bullhead catfish (Ameiurus natalis) in order to determine factors contributing to the bioconcentration of this fission product. Bullhead catfish (N = 173) were obtained from the Pond B reservoir, Savannah River Site, SC. Muscle 137Cs concentration did not differ between sexes (ANOVA, P = 0.631). Age of fish (1--6 yr) appeared to explain variation in 137Cs concentration (P = 0.005), but not when mass of fish was included as a model covariate (P = 0.086). Least-square means, adjusted for the covariate, suggested that 1 and 2 yr old fish (71.09 and 81.38 mean pCi/g, respectively) differed from 3, 4, 5, and 6 yr old fish (94.94, 92.34, 97.27, 90.25 mean pCi/g, respectively). Therefore, the effects of age and mass were evaluated by partitioning the data into young (1--2 yr) and older (3, 4, 5, and 6 yr) age classes. When adjusted for mass, age (young vs. old) significantly accounted for differences in 137Cs concentration (78.98 vs. 94.33 mean pCi/g; P = 0.005). Within older fish, neither age (P = 0.870) or mass (P = 0.361) significantly influenced 137Cs concentration. The effect of age within young fish was nonsignificant (P = 0.971) in contrast to the influence of mass (P = 0.044). This suggests that the contribution of 'size' (mass) to 137Cs body burden is related to early growth and that these fish reach an asymptotic 'steady-state' 137Cs concentration by 3 years of age

  12. Retinoic acid triggers meiosis initiation via stra8-dependent pathway in Southern catfish, Silurus meridionalis.

    Science.gov (United States)

    Li, Minghui; Feng, Ruijuan; Ma, He; Dong, Ranran; Liu, Zhilong; Jiang, Wentao; Tao, Wenjing; Wang, Deshou

    2016-06-01

    Existing studies demonstrated that retinoic acid (RA) regulates meiotic initiation via stra8-independent pathway in teleosts which lack stra8 in their genomes. However, stra8 was recently identified from several fish species including Southern catfish (Silurus meridionalis). To explore the existence of stra8-dependent pathway in RA mediated meiotic initiation in fishes, in the present study, the genes encoding RA synthase aldh1a2 and catabolic enzyme cyp26a1 and cyp26b1 were cloned from the Southern catfish. By immunohistochemistry, Aldh1a2 signal was observed in gonads of both sexes during the meiotic initiation period. By real-time PCR, differentially expressed gene was observed for cyp26a1, but not for cyp26b1, in gonads during the meiotic initiation. Administration of exogenous RA or inhibition of endogenous RA degradation by either KET (RA catabolic enzyme inhibitor) or cyp26a1 knockdown using CRISPR/Cas9 induced advanced meiotic initiation in the ovaries as demonstrated by increased Stra8/stra8 expression and appearance of oocytes. In contrast, treatment with RA synthase inhibitor DEAB resulted in delayed meiotic initiation and Stra8/stra8 expression in the ovaries, which was rescued by exogenous RA administration. These results indicated that (1) RA triggers the onset of meiosis via stra8-dependent pathway in stra8 existing teleosts, as it does in tetrapods; (2) exogenous RA can rescue the endogenous RA deficiency; (3) Cyp26a1, instead of Cyp26b1, is the key catabolic enzyme involved in meiosis initiation in teleosts. Taken together, RA might trigger meiotic initiation via stra8-dependent and -independent pathway in different teleosts. PMID:26764212

  13. Molecular systematic and historical biogeography of the armored Neotropical catfishes Hypoptopomatinae and Neoplecostominae (Siluriformes: Loricariidae).

    Science.gov (United States)

    Chiachio, Márcio Cesar; Oliveira, Claudio; Montoya-Burgos, Juan I

    2008-11-01

    The Neotropics possess the greatest freshwater fish diversity of the world, rendering the study of their evolutionary history extremely challenging. Loricariidae catfishes are one of the most diverse components of the Neotropical ichthyofauna and despite a long history of classification, major issues still need elucidation. Based on a nuclear gene, we present a robust phylogeny of two former loricariid subfamilies: Hypoptopomatinae and Neoplecostominae. Our results show that Neoplecostominae is nested within Hypoptopomatinae, and is the sister group to the former Otothyrini tribe. According to our results, supplemented by morphological observations, we erect two new subfamilies, the Otothyrinae and a new Hypoptopomatinae, and modify the Neoplecostominae by including the genus Pseudotocinclus. The uncovered evolutionary relationships allow a detailed analysis of their historical biogeography. We tested two Dispersal-Extinction-Cladogenesis models for inferring the distribution range evolution of the new subfamilies, and show that the model having no constrains performs better than a model constraining long-range dispersal. The Maximum Likelihood reconstructions of ancestral ranges showed a marked division between the Amazonian origin of the Hypoptopomatinae and the eastern coastal Brazil+Upper Paraná origin of the Neoplecostominae and Otothyrinae. Markedly few instances of dispersal across the border separating the Amazon basin and the Paraná-Paraguay+eastern coastal Brazil+Uruguay were reconstructed. This result is in clear contrast with the historical biogeography of many Neotropical fishes, including other Loricariidae. Part of the dispersal limitation may be explained by divergent ecological specialization: lowland rivers versus mountain streams habitats. Moreover, because most species of the new subfamilies are small, we hypothesize that body size-related effects might limit their dispersal, like predation and energetic cost to migration. Finally

  14. New species and geographical records of dactylogyrids (Monogenea) of catfish (Siluriformes) from the Peruvian Amazonia.

    Science.gov (United States)

    Mendoza-Palmero, Carlos A; Scholz, Tomáš; Mendoza-Franco, Edgar F; Kuchta, Roman

    2012-06-01

    Three new species of gill monogeneans (Dactylogyridae: Ancyrocephalinae) are described from siluriform catfish from Iquitos, Peru: Demidospermus mortenthaleri n. sp. from Brachyplatystoma juruense (Boulenger), Demidospermus brevicirrus n. sp. from Pimelodus sp., and Aphanoblastella aurorae n. sp. from Goeldiella eques (Müller & Troschel). Demidospermus mortenthaleri is characterized by a male copulatory organ (MCO) with a small loop at its middle portion; 2 types of hooks, of which pairs 5 and 6 are longer than the remaining hooks; a proximal subunit round and highly depressed thumb; and a sclerotized vagina with a round pad at the vaginal aperture. Demidospermus brevicirrus is distinguished from other congeners by the presence of a short, straight, and robust MCO and boot-shaped accessory piece with a hooked projection directed posteriorly. Aphanoblastella aurorae is the only species of the genus that possesses an arrow-shaped sclerotized vagina and a medial process on the dorsal bar. Another 6 dactylogyrids described previously are recorded for the first time from the Peruvian Amazonia: Cosmetocleithrum bulbocirrus Kritsky, Thatcher and Boeger, 1986 ; Vancleaveus fungulus Kritsky, Thatcher and Boeger, 1986 ; V. janauacaensis Kritsky, Thatcher and Boeger, 1986 ; V. platyrhynchi Kritsky, Thatcher and Boeger, 1986 ; Unilatus unilatus Mizelle and Kritsky, 1967 ; and U. brittani Mizelle, Kritsky and Crane, 1968 . Based on observations of specimens collected in the Peruvian Amazonia, new morphological data for these species are provided. Comparison of new specimens of U. unilatus and U. brittani with those of Unilatus brevispinus Suriano, 1985 and Unilatus longispinus Suriano, 1985 , both originally described from Brazil, has shown that they are conspecific. Therefore, the latter species were synonymized with U. unilatus and U. brittani , respectively. In addition, 56 undescribed monogeneans found in catfish from the Peruvian Amazonia, some of them probably belonging

  15. Ecological impacts of fluridone and copper sulphate in catfish aquaculture ponds.

    Science.gov (United States)

    Jacob, Annie P; Culver, David A; Lanno, Roman P; Voigt, Astrid

    2016-05-01

    Fluridone and copper sulphate are often used for controlling macrophytes and algae in aquaculture ponds. The present study examined the ecological effects of these chemicals on macrophyte, phytoplankton, and zooplankton biomass; plankton community structure; water quality parameters; and fish survival and yield in catfish culture ponds using a randomized complete block design. The estimated half-life of fluridone in the individual ponds ranged from 1.6 d to 10.8 d. Free copper ion activity in ponds treated with copper sulphate was dynamic, ranging from pCu of 7.7 to 8.9 after each application and decreasing to approximately 12 (1 × 10(-12)  M) within 1 wk after each application, approaching observed values in control ponds (pCu = 12.3-13.4). No difference in macrophyte biomass was observed among treatments. Fluridone and copper treatments elicited different responses within the phytoplankton community. Copper treatments reduced Cyanophyta biomass but increased biomass of more tolerant taxa among the Chlorophyta and Chrysophyta. Fluridone treatments reduced total phytoplankton biomass including Cyanophyta and increased the sensitivity of Chlorophyta and Chrysophyta to copper. Copper also affected zooplankton community composition as a result of direct toxic effects on sensitive zooplankton taxa (e.g., Cladocera), whereas Copepoda biomass in copper-treated ponds exceeded that in controls. Catfish survival and yield were not significantly different among treatments. The results of the present study suggest that fluridone and copper interact at realistic application rates, increasing the ability to control algae compared with treatments where they are applied alone. Environ Toxicol Chem 2016;35:1183-1194. © 2015 SETAC. PMID:26395963

  16. Bioconcentration and endocrine disruption effects of diazepam in channel catfish, Ictalurus punctatus.

    Science.gov (United States)

    Overturf, C L; Overturf, M D; Huggett, D B

    2016-01-01

    Recently, the detection of pharmaceuticals in surface waters has increased worldwide. Pharmaceuticals are typically found in the environment at concentrations well below therapeutic levels in humans; however, their mechanisms of action may be largely unknown in non-target organisms, such as teleost species. Thus, chronic exposure to these types of compounds warrants further investigation. The goal of this study was to examine the potential for diazepam, a model benzodiazepine drug, to bioconcentrate in tissues of channel catfish and to examine its ability to interact with the endocrine system through modulation of steroid hormones and/or steroidogenic genes. To investigate the bioconcentration potential of diazepam, channel catfish (Ictalurus punctatus) were exposed to 1 ng/mL diazepam for seven days, followed by clean water for another seven days, using an abbreviated OECD 305 Fish Bioconcentration Test study design. This concentration of diazepam is well below environmentally relevant concentrations of diazepam (ng/L). To evaluate steroidogenic effects, fish were exposed to 1 ng/mL diazepam for seven days only. Steroid hormone concentrations were analyzed for various tissues, as well as expression of selected steroidogenic genes. Calculated bioconcentration factors for diazepam were well below regulatory threshold values in all tissues analyzed. No changes in steroid hormone concentration were detected in any tissue analyzed; however, the steroidogenic gene cytochrome P450 side chain cleavage (P450scc) was significantly down-regulated at day 5 and 3β-hydroxy steroid dehydrogenase (3β-HSD) was significantly down-regulated at day 7 in the gonad. These results indicate that although diazepam does not significantly bioconcentrate, low-level chronic exposure to diazepam may have the potential to interact with endocrine function by altering gene expression. PMID:26875913

  17. Changes in brain monoamine levels and monoamine oxidase activity in the catfish, Clarias batrachus, during chronic treatments with mercurials

    International Nuclear Information System (INIS)

    In mammals, the central nervous system is the primary target for CH3Hg poisoning which is clinically known as Minamata disease. Hg is a widely recognized neurotoxin and has been reported to impair brain monoamine neurotransmitter metabolism. Reports on effects of Hg on brain monoamine activity in fishes are scarce. In the present study, therefore, changes in the brain monoamine levels and the degradation enzyme, monoamine oxidase (MAO), are described in the catfish, Clarias batrachus, exposed to sublethal concentrations of mercuric chloride (HgCl2-inorganic Hg), methylmercuric chloride (CH3HgCl-organic Hg), and a commercial mercurial fungicide formulation, emisan 6 (methoxyethyl Hg-organic Hg) for 45, 90 and 180 d during gonadal recrudescence. These intervals correspond to late preparatory, prespawning and spawning phases, respectively, of the annual reproductive cycle of the catfish

  18. Genetic variability analysis of Giant river catfish (Sperata seenghala) populations from Indus river system by RAPD-PCR.

    Science.gov (United States)

    Saini, A; Dua, A; Mohindra, V

    2010-08-01

    The Giant river catfish, Sperata seenghala (Sykes) is commercially very important fish species of South Asia. Genetic variability between its populations collected from two rivers i.e. river Sutlej and river Beas of Indus river system in India were examined using randomly amplified polymorphic DNA analysis. Total 38 fish samples were collected from river Sutlej whereas 46 fish samples were collected from river Beas. Total 40 primers were screened, out of these 7 were selected for studying polymorphism which produced a total of 64 RAPD loci in two populations. Percentage polymorphic loci calculated following 95% criterion was 89.06% for Beas population as compared to 95.31% for Sutlej population. Moderate level of genetic divergence (genetic distance of 0.0486) between both the populations suggests distinct population substructure of giant river catfish in both the rivers. PMID:20873207

  19. Occurrence and intensity of parasites in Wels catfish, Silurus glanis L. 1758 from Amirkelayeh wetland, southwest of the Caspian Sea.

    Science.gov (United States)

    Khara, Hossein; Sattari, Masoud

    2016-09-01

    Amirkelayeh international wetland with a surface area of 1,230 hectares and fifteen fish species is an important water resource in the southern part of the Caspian Sea. There is few reports on fish parasites of this area. In the present study, a total of 80 Wels catfish, Silurus glanis, were collected from April through July 2011. After recording biometric characteristics, common necropsy and parasitology methods were used. In the present study, a total of 441 individuals of seven parasite species consisting of one nematode: Raphidascaroides sp., one cestode: Triaenophorus crassus, two digenean trematodes: Aphanurus stossichi and Diplostomum spathaceum, two monogenean trematodes: Silurodiscoides vistulensis and Silurodiscoides siluri, and one crustacean: copepodid stage of Lernaea cyprinacea was found in the catfish. The occurrence of copepodid stage of L. cyprinacea, Raphidascaroides sp. and T. crassus are reported for the first time from S. glanis in Iran. PMID:27605796

  20. Discovering the African freshwater "torpedo": legendary Ethiopia, religious controversies, and a catfish capable of reanimating dead fish.

    Science.gov (United States)

    Piccolino, Marco; Finger, Stanley; Barbara, Jean-Gaël

    2011-07-01

    The electric catfishes of African rivers and lakes, once depicted on Egyptian tomb art, have been largely overlooked in histories and reviews of electric fish biology and animal electricity. This article examines how Westerners, especially Dominican and Jesuit missionaries, discovered them in Ethiopia and other parts of Africa at the beginning of the seventeenth century. What transpired took place against the backdrop of tales involving the Bible, Prester John's mythical empire, and imaginary animals with fabulous powers. In effect, how they were found is related to attempts to convert Ethiopian Christians to true Catholicism, hopes of discovering great riches, and opportunities to trade, and not with the efforts of skilled natural philosophers to document and conduct experiments on the wildlife of this continent. Nevertheless, the early descriptions by Europeans circulated, and during the next century these catfishes began to be used in experiments that helped to make animal electricity a reality. PMID:21736441