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Sample records for castor canadensis population

  1. Coupled stream and population dynamics: Modeling the role beaver (Castor canadensis) play in generating juvenile steelhead (Oncorhynchus mykiss) habitat

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    Jordan, C.; Bouwes, N.; Wheaton, J. M.; Pollock, M.

    2013-12-01

    Over the past several centuries, the population of North American Beaver has been dramatically reduced through fur trapping. As a result, the geomorphic impacts long-term beaver occupancy and activity can have on fluvial systems have been lost, both from the landscape and from our collective memory such that physical and biological models of floodplain system function neither consider nor have the capacity to incorporate the role beaver can play in structuring the dynamics of streams. Concomitant with the decline in beaver populations was an increasing pressure on streams and floodplains through human activity, placing numerous species of stream rearing fishes in peril, most notably the ESA listing of trout and salmon populations across the entirety of the Western US. The rehabilitation of stream systems is seen as one of the primary means by which population and ecosystem recovery can be achieved, yet the methods of stream rehabilitation are applied almost exclusively with the expected outcome of a static idealized stream planform, occasionally with an acknowledgement of restoring processes rather than form and only rarely with the goal of a beaver dominated riverscape. We have constructed an individual based model of trout and beaver populations that allows the exploration of fish population dynamics as a function of stream habitat quality and quantity. We based the simulation tool on Bridge Creek (John Day River basin, Oregon) where we have implemented a large-scale restoration experiment using wooden posts to provide beavers with stable platforms for dam building and to simulate the dams themselves. Extensive monitoring captured geomorphic and riparian changes, as well as fish and beaver population responses; information we use to parameterize the model as to the geomorphic and fish response to dam building beavers. In the simulation environment, stream habitat quality and quantity can be manipulated directly through rehabilitation actions and indirectly

  2. Development of nine new microsatellite loci for the American beaver, Castor canadensis (Rodentia: Castoridae), and cross-species amplification in the European beaver, Castor fiber.

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    Pelz-Serrano, Karla; Munguia-Vega, Adrian; Piaggio, Antoinette J; Neubaum, Melissa; Munclinger, Pavel; Pártl, Adam; VAN Riper Iii, Charles; Culver, Melanie

    2009-03-01

    We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cross-species amplification in Castor fiber was successful only in four loci, we tested also nine recently published C. canadensis loci in the Eurasian species. Eight of the published loci amplified; however, three were monomorphic. The number of alleles was lower in C. fiber than in C. canadensis at all loci tested. © 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd.

  3. Characterization of the North American beaver (Castor canadensis) papillomavirus genome.

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    Rogovskyy, Artem S; Chen, Zigui; Burk, Robert D; Bankhead, Troy

    2014-01-10

    The papillomaviruses comprise a large group of viruses that cause proliferations of the stratified squamous epithelium of skin and mucosa in a variety of animals. An earlier report identified a novel papillomavirus of the North American beaver, Castor canadensis (CcanPV1) that was associated with cutaneous exophytic lesions. In the current study, we determined the sequence of the complete 7435 basepair genome of CcanPV1. The genome contains an Upstream Regulatory Region located between the end of L1 and the start of E6, and seven canonical papillomavirus open reading frames encoding five early (E6, E7, E1, E2, and E4) and two late (L2 and L1) proteins. No E5 open reading frame was detected. Phylogenetic analysis of the CcanPV1 genome places the virus between the genera Kappapapillomavirus and Mupapillomavirus. Analyses of the papillomavirus genomes detected in different species of the order Rodentia indicate these viruses do not form a monophyletic clade. Copyright © 2013 Elsevier B.V. All rights reserved.

  4. Where and How Wolves (Canis lupus Kill Beavers (Castor canadensis.

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    Thomas D Gable

    Full Text Available Beavers (Castor canadensis can be a significant prey item for wolves (Canis lupus in boreal ecosystems due to their abundance and vulnerability on land. How wolves hunt beavers in these systems is largely unknown, however, because observing predation is challenging. We inferred how wolves hunt beavers by identifying kill sites using clusters of locations from GPS-collared wolves in Voyageurs National Park, Minnesota. We identified 22 sites where wolves from 4 different packs killed beavers. We classified these kill sites into 8 categories based on the beaver-habitat type near which each kill occurred. Seasonal variation existed in types of kill sites as 7 of 12 (58% kills in the spring occurred at sites below dams and on shorelines, and 8 of 10 (80% kills in the fall occurred near feeding trails and canals. From these kill sites we deduced that the typical hunting strategy has 3 components: 1 waiting near areas of high beaver use (e.g., feeding trails until a beaver comes near shore or ashore, 2 using vegetation, the dam, or other habitat features for concealment, and 3 immediately attacking the beaver, or ambushing the beaver by cutting off access to water. By identifying kill sites and inferring hunting behavior we have provided the most complete description available of how and where wolves hunt and kill beavers.

  5. Prevalence of agglutinating antibodies to Toxoplasma gondii and Sarcocystis neurona in beavers (Castor canadensis) from Massachusetts

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    Jordan, C.N.; Kaur, T.; Koenen, K.; DeStefano, S.; Zajac, A.M.; Lindsay, D.S.

    2005-01-01

    The present study examined the seroprevalence of Toxoplasma gondii and Sarcocystls neurona in a population of beavers (Castor canadensis) from Massachusetts. Sixty-two blood samples were collected during the field seasons over 3 consecutive years from different animals. Blood was collected onto filter paper and shipped to the Department of Biomedical Sciences, Virginia Tech, Blacksburg, Virginia, for parasite testing. The samples were tested at dilutions of 1:25, 1:50, and 1:100 against each parasite antigen by modified agglutination tests to determine whether antibodies to either parasite were present in the blood. Six of 62 samples (10%) were positive for T. gondii, with 2 samples having titers of 1:25 and 4 having titers of 1:50. Four of 62 samples (6%) were positive for S. neurona, with 2 samples having titers of 1:25 and 2 having titers of 1:50. ?? American Society of Pathologists 2005.

  6. Manipulating beaver (Castor canadensis) feeding responses to invasive tamarisk (Tamarix spp.).

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    Kimball, Bruce A; Perry, Kelly R

    2008-08-01

    To evaluate methods for promoting consumption of tamarisk plants by beavers (Castor canadensis), we determined the feeding responses by captive beavers to diets that contained tannins and sodium chloride (hereafter referred to as tamarisk diet). In two-choice tests, beavers consumed equivalent quantities of tamarisk diet and control diet. Treatment with polyethylene glycol and fructose did not increase beaver preferences for the tamarisk diet. When offered the choice of control diet and casein hydrolysate-treated control diet, beavers strongly avoided the latter, showing feeding deterring activity of casein hydrolysate. However, when tamarisk diet was the alternative to the deterrent treatment, beavers consumed similar quantities of the two diets. Finally, beaver foraging preferences for actual plant cuttings were assessed. Casein hydrolysate application to cuttings of black poplar (Populus nigra) and Scouler's willow (Salix scouleriana) reduced browsing of these highly preferred species and promoted a marked increase in browsing of tamarisk (Tamarix ramosissima). These results suggest that casein hydrolysate treatment of desirable riparian plant species such as Salix and Populus may promote beaver foraging of invasive tamarisk.

  7. A novel papillomavirus isolated from proliferative skin lesions of a wild American beaver (Castor canadensis).

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    Rogovskyy, Artem S; Baszler, Timothy V; Bradway, Daniel S; Bruning, Darren L; Davitt, Christine M; Evermann, James F; Burk, Robert D; Chen, Zigui; Mansfield, Kristin G; Haldorson, Gary J

    2012-07-01

    Cutaneous papillomatosis was diagnosed in an adult American beaver (Castor canadensis). Gross lesions included numerous exophytic, roughly circular, lightly pigmented lesions on hairless areas of fore and hind feet and the nose. The most significant histopathologic findings were multifocal papilliform hyperplasia of the superficial stratified squamous epithelium, with multifocal koilocytes, and multiple cells with large, darkly basophilic intranuclear inclusion bodies. A virus with properties consistent with papillomavirus (PV) was recovered by virus isolation of skin lesions, utilizing rabbit and feline kidney cell lines. The presence of the virus was confirmed by PV-specific polymerase chain reaction. The partial sequences of E1 and L1 genes did not closely match those of any PVs in GenBank, suggesting that this might be a new type of PV. Partial E1 and L1 nucleotide sequences of the beaver papillomavirus (hereafter, ARbeaver-PV1) were used to create a phylogenetic tree employing the complete E1 and L1 open reading frame nucleotide sequences of 68 PVs. The phylogenetic tree placed the ARbeaver-PV1 in a clade that included the Mupapillomavirus (HPV1 and HPV63) and Kappapapillomavirus (OcPV1 and SfPV1) genera. The present article confirms the papillomaviral etiology of cutaneous exophytic lesions in the beaver.

  8. Design to monitor trend in abundance and presence of American beaver (Castor canadensis) at the national forest scale.

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    Beck, Jeffrey L; Dauwalter, Daniel C; Gerow, Kenneth G; Hayward, Gregory D

    2010-05-01

    Wildlife conservationists design monitoring programs to assess population dynamics, project future population states, and evaluate the impacts of management actions on populations. Because agency mandates and conservation laws call for monitoring data to elicit management responses, it is imperative to design programs that match the administrative scale for which management decisions are made. We describe a program to monitor population trends in American beaver (Castor canadensis) on the US Department of Agriculture, Black Hills National Forest (BHNF) in southwestern South Dakota and northeastern Wyoming, USA. Beaver have been designated as a management indicator species on the BHNF because of their association with riparian and aquatic habitats and its status as a keystone species. We designed our program to monitor the density of beaver food caches (abundance) within sampling units with beaver and the proportion of sampling units with beavers present at the scale of a national forest. We designated watersheds as sampling units in a stratified random sampling design that we developed based on habitat modeling results. Habitat modeling indicated that the most suitable beaver habitat was near perennial water, near aspen (Populus tremuloides) and willow (Salix spp.), and in low gradient streams at lower elevations. Results from the initial monitoring period in October 2007 allowed us to assess costs and logistical considerations, validate our habitat model, and conduct power analyses to assess whether our sampling design could detect the level of declines in beaver stated in the monitoring objectives. Beaver food caches were located in 20 of 52 sampled watersheds. Monitoring 20 to 25 watersheds with beaver should provide sufficient power to detect 15-40% declines in the beaver food cache index as well as a twofold decline in the odds of beaver being present in watersheds. Indices of abundance, such as the beaver food cache index, provide a practical measure of

  9. Bacterial and Archaeal Diversity in the Gastrointestinal Tract of the North American Beaver (Castor canadensis.

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    Robert J Gruninger

    Full Text Available The North American Beaver (Castor canadensis is the second largest living rodent and an iconic symbol of Canada. The beaver is a semi-aquatic browser whose diet consists of lignocellulose from a variety of plants. The beaver is a hindgut fermenter and has an enlarged ceacum that houses a complex microbiome. There have been few studies examining the microbial diversity in gastrointestinal tract of hindgut fermenting herbivores. To examine the bacterial and archaeal communities inhabiting the gastrointestinal tract of the beaver, the microbiome of the ceacum and feaces was examined using culture-independent methods. DNA from the microbial community of the ceacum and feaces of 4 adult beavers was extracted, and the16S rRNA gene was sequenced using either bacterial or archaeal specific primers. A total of 1447 and 1435 unique bacterial OTUs were sequenced from the ceacum and feaces, respectively. On average, the majority of OTUs within the ceacum were classified as Bacteroidetes (49.2% and Firmicutes (47.6%. The feaces was also dominated by OTUs from Bacteroidetes (36.8% and Firmicutes (58.9%. The composition of bacterial community was not significantly different among animals. The composition of the ceacal and feacal microbiome differed, but this difference is due to changes in the abundance of closely related OTUs, not because of major differences in the taxonomic composition of the communities. Within these communities, known degraders of lignocellulose were identified. In contrast, to the bacterial microbiome, the archaeal community was dominated by a single species of methanogen, Methanosphaera stadtmanae. The data presented here provide the first insight into the microbial community within the hindgut of the beaver.

  10. De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis

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    Si Lok

    2017-02-01

    Full Text Available The Canadian beaver (Castor canadensis is the largest indigenous rodent in North America. We report a draft annotated assembly of the beaver genome, the first for a large rodent and the first mammalian genome assembled directly from uncorrected and moderate coverage (< 30 × long reads generated by single-molecule sequencing. The genome size is 2.7 Gb estimated by k-mer analysis. We assembled the beaver genome using the new Canu assembler optimized for noisy reads. The resulting assembly was refined using Pilon supported by short reads (80 × and checked for accuracy by congruency against an independent short read assembly. We scaffolded the assembly using the exon–gene models derived from 9805 full-length open reading frames (FL-ORFs constructed from the beaver leukocyte and muscle transcriptomes. The final assembly comprised 22,515 contigs with an N50 of 278,680 bp and an N50-scaffold of 317,558 bp. Maximum contig and scaffold lengths were 3.3 and 4.2 Mb, respectively, with a combined scaffold length representing 92% of the estimated genome size. The completeness and accuracy of the scaffold assembly was demonstrated by the precise exon placement for 91.1% of the 9805 assembled FL-ORFs and 83.1% of the BUSCO (Benchmarking Universal Single-Copy Orthologs gene set used to assess the quality of genome assemblies. Well-represented were genes involved in dentition and enamel deposition, defining characteristics of rodents with which the beaver is well-endowed. The study provides insights for genome assembly and an important genomics resource for Castoridae and rodent evolutionary biology.

  11. Bacterial and Archaeal Diversity in the Gastrointestinal Tract of the North American Beaver (Castor canadensis).

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    Gruninger, Robert J; McAllister, Tim A; Forster, Robert J

    2016-01-01

    The North American Beaver (Castor canadensis) is the second largest living rodent and an iconic symbol of Canada. The beaver is a semi-aquatic browser whose diet consists of lignocellulose from a variety of plants. The beaver is a hindgut fermenter and has an enlarged ceacum that houses a complex microbiome. There have been few studies examining the microbial diversity in gastrointestinal tract of hindgut fermenting herbivores. To examine the bacterial and archaeal communities inhabiting the gastrointestinal tract of the beaver, the microbiome of the ceacum and feaces was examined using culture-independent methods. DNA from the microbial community of the ceacum and feaces of 4 adult beavers was extracted, and the16S rRNA gene was sequenced using either bacterial or archaeal specific primers. A total of 1447 and 1435 unique bacterial OTUs were sequenced from the ceacum and feaces, respectively. On average, the majority of OTUs within the ceacum were classified as Bacteroidetes (49.2%) and Firmicutes (47.6%). The feaces was also dominated by OTUs from Bacteroidetes (36.8%) and Firmicutes (58.9%). The composition of bacterial community was not significantly different among animals. The composition of the ceacal and feacal microbiome differed, but this difference is due to changes in the abundance of closely related OTUs, not because of major differences in the taxonomic composition of the communities. Within these communities, known degraders of lignocellulose were identified. In contrast, to the bacterial microbiome, the archaeal community was dominated by a single species of methanogen, Methanosphaera stadtmanae. The data presented here provide the first insight into the microbial community within the hindgut of the beaver.

  12. Signatures of self-assembly in size distributions of wood members in dam structures of Castor canadensis

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    David M. Blersch

    2014-12-01

    Full Text Available Beavers (Castor canadensis construct dams on rivers throughout most of their historical range in North America, and their impact on water patterns in the landscape is considerable. Dam formation by beavers involves two processes: (1 intentional construction through the selection and placement of wood and sediment, which facilitates (2 the passive capture and accretion of suspended wood and sediment. The second process is a self-assembly mechanism that the beavers leverage by utilizing energy subsidies of watershed transport processes. The relative proportion of beaver activity to self-assembly processes in dam construction, however, is unknown. Here we show that lotic self-assembly processes account for a substantial portion of the work expended in beaver dam construction. We found through comprehensive measurement of the stick dimensions that the distributions for diameter, length, and volume are log-normal. By noting evidence of teeth markings, we determined that size distributions skewed significantly larger for wood handled by beavers compared to those that were not. Subsequent mass calculations suggest that beavers perform 50%–70% of the work of wood member placement for dam assembly, with riparian self-assembly processes contributing the remainder. Additionally, our results establish a benchmark for assessing the proportion of self-assembly work in similar riparian structures. Keywords: Beaver dam, Construction, Castor canadensis, Self-assembly, Distribution, Wood

  13. Antigen detection, rabies virus isolation, and Q-PCR in the quantification of viral load in a natural infection of the North American beaver (Castor canadensis).

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    Morgan, Shannon M D; Pouliott, Craig E; Rudd, Robert J; Davis, April D

    2015-01-01

    All mammals are believed susceptible to rabies virus infection, yet transmission from nonreservoir hosts to humans is uncommon. However, interactions between nonreservoir hosts and humans occur frequently and risk of exposure increases where rabies is enzootic. We describe rabies and apparent pantropism of rabies virus in a beaver (Castor canadensis).

  14. Population genetic structure in natural and reintroduced beaver (Castor fiber populations in Central Europe

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    Kautenburger, R.

    2008-12-01

    Full Text Available Castor fiber Linnaeus, 1758 is the only indigenous species of the genus Castor in Europe and Asia. Due to extensive hunting until the beginning of the 20th century, the distribution of the formerly widespread Eurasian beaver was dramatically reduced. Only a few populations remained and these were in isolated locations, such as the region of the German Elbe River. The loss of genetic diversity in small or captive populations throughgenetic drift and inbreeding is a severe conservation problem. However, the reintroduction of beaver populations from several regions in Europe has shown high viability and populations today are growing fast. In the present study we analysed the population genetic structure of a natural and two reintroduced beaver populations in Germany and Austria. Furthermore, we studied the genetic differentiation between two beaver species, C. fiber and the American beaver (C. canadensis, using RAPD (Random Amplified Polymorphic DNA as a genetic marker. The reintroduced beaver populations of different origins and the autochthonous population of the Elbe River showed a similar low genetic heterogeneity. There was an overall high genetic similarity in the species C. fiber, and no evidence was found for a clear subspecific structure in the populations studied.

  15. Assessment of a new Bacteroidales marker targeting North American beaver (Castor canadensis) fecal pollution by real-time PCR.

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    Marti, Romain; Zhang, Yun; Tien, Yuan-Ching; Lapen, David R; Topp, Edward

    2013-11-01

    In many settings wildlife can be a significant source of fecal pathogen input into surface water. The North American beaver (Castor canadensis) is a zoonotic reservoir for several human pathogens including Cryptosporidium spp. and Giardia spp. In order to specifically detect fecal pollution by beavers, we have developed and validated a beaver-specific Bacteroidales marker, designated Beapol01, based on the 16S rRNA gene. The marker is suitable for quantifying pollution using real-time PCR. The specificity and sensitivity of the marker was excellent, Beaver signal was detected in water of a mixed-activity watershed harbouring this rodent. Overall, Beapol01 will be useful for a better understanding of fecal source inputs in drainage basins inhabited by the beaver. © 2013.

  16. Substrate-Driven Convergence of the Microbial Community in Lignocellulose-Amended Enrichments of Gut Microflora from the Canadian Beaver (Castor canadensis) and North American Moose (Alces americanus).

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    Wong, Mabel T; Wang, Weijun; Lacourt, Michael; Couturier, Marie; Edwards, Elizabeth A; Master, Emma R

    2016-01-01

    Strategic enrichment of microcosms derived from wood foragers can facilitate the discovery of key microbes that produce enzymes for the bioconversion of plant fiber (i.e., lignocellulose) into valuable chemicals and energy. In this study, lignocellulose-degrading microorganisms from the digestive systems of Canadian beaver (Castor canadensis) and North American moose (Alces americanus) were enriched under methanogenic conditions for over 3 years using various wood-derived substrates, including (i) cellulose (C), (ii) cellulose + lignosulphonate (CL), (iii) cellulose + tannic acid (CT), and (iv) poplar hydrolysate (PH). Substantial improvement in the conversion of amended organic substrates into biogas was observed in both beaver dropping and moose rumen enrichment cultures over the enrichment phases (up to 0.36-0.68 ml biogas/mg COD added), except for enrichments amended with tannic acid where conversion was approximately 0.15 ml biogas/mg COD added. Multiplex-pyrosequencing of 16S rRNA genes revealed systematic shifts in the population of Firmicutes, Bacteroidetes, Chlorobi, Spirochaetes, Chloroflexi, and Elusimicrobia in response to the enrichment. These shifts were predominantly substrate driven, not inoculum driven, as revealed by both UPGMA clustering pattern and OTU distribution. Additionally, the relative abundance of multiple OTUs from poorly defined taxonomic lineages increased from less than 1% to 25-50% in microcosms amended with lignocellulosic substrates, including OTUs from classes SJA-28, Endomicrobia, orders Bacteroidales, OPB54, and family Lachnospiraceae. This study provides the first direct comparison of shifts in microbial communities that occurred in different environmental samples in response to multiple relevant lignocellulosic carbon sources, and demonstrates the potential of enrichment to increase the abundance of key lignocellulolytic microorganisms and encoded activities.

  17. Identificación de los mecanismos subyacentes a la invasión de Castor canadensis (Rodentia en el archipiélago de Tierra del Fuego, Chile Identifying the mechanisms underlying the invasion of Castor canadensis (Rodentia into Tierra del Fuego archipelago, Chile

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    PETRA K WALLEM

    2007-09-01

    conceptual sobre el cual se pueden desarrollar futuras investigaciones referentes a esta invasiónCastor canadensis is considered an archetypical ecosystem engineer, which modifies the state of biotic and abiotic factors through non-trophic interactions. This species was introduced by the Argentinean government into Tierra del Fuego island in 1946, and subsequently colonized autonomously the neighboring islands of Navarino, Dawson and Hoste. Currently this invader occupies contrasting ecosystems such as the Magellan subpolar beech forest and Patagonian scrub and steppe. This ability to colonize contrasting habitats suggests that beaver expansion will be limited mainly by hydrological resources, threatening to colonize the complete extent of temperate beech forests on the mainland (from 35 to 55° S. The present review proposes three hypotheses regarding the underlying mechanisms to this successful invasion: natural enemy escape, resource opportunities, and self-facilitation through non-trophic interactions. Current knowledge regarding beaver colonization and foraging behavior (e.g., habitat selection independently of forest availability, irruptive population growth, and apparent selective exploitation of Nothofagus pumilio, dominant species in the Magellan forest suggests that enemy escape and resource opportunity are the main mechanisms underlying this invasion. The observation of higher densities of active colonies, where the extent of beaver habitat modification is larger, suggests that self-facilitation may be relevant to the success of this invasion. Current information does not allow testing these hypotheses, but it provides a framework to develop future investigations regarding this invasion in Tierra del Fuego

  18. Modeling intrinsic potential for beaver (Castor canadensis) habitat to inform restoration and climate change adaptation

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    Dittbrenner, Benjamin J.; Pollack, Michael M.; Schilling, Jason W.; Olden, Julian D.; Lawler, Joshua J.; Torgersen, Christian E.

    2018-01-01

    Through their dam-building activities and subsequent water storage, beaver have the potential to restore riparian ecosystems and offset some of the predicted effects of climate change by modulating streamflow. Thus, it is not surprising that reintroducing beaver to watersheds from which they have been extirpated is an often-used restoration and climate-adaptation strategy. Identifying sites for reintroduction, however, requires detailed information about habitat factors—information that is not often available at broad spatial scales. Here we explore the potential for beaver relocation throughout the Snohomish River Basin in Washington, USA with a model that identifies some of the basic building blocks of beaver habitat suitability and does so by relying solely on remotely sensed data. More specifically, we developed a generalized intrinsic potential model that draws on remotely sensed measures of stream gradient, stream width, and valley width to identify where beaver could become established if suitable vegetation were to be present. Thus, the model serves as a preliminary screening tool that can be applied over relatively large extents. We applied the model to 5,019 stream km and assessed the ability of the model to correctly predict beaver habitat by surveying for beavers in 352 stream reaches. To further assess the potential for relocation, we assessed land ownership, use, and land cover in the landscape surrounding stream reaches with varying levels of intrinsic potential. Model results showed that 33% of streams had moderate or high intrinsic potential for beaver habitat. We found that no site that was classified as having low intrinsic potential had any sign of beavers and that beaver were absent from nearly three quarters of potentially suitable sites, indicating that there are factors preventing the local population from occupying these areas. Of the riparian areas around streams with high intrinsic potential for beaver, 38% are on public lands and 17

  19. Modeling intrinsic potential for beaver (Castor canadensis) habitat to inform restoration and climate change adaptation.

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    Dittbrenner, Benjamin J; Pollock, Michael M; Schilling, Jason W; Olden, Julian D; Lawler, Joshua J; Torgersen, Christian E

    2018-01-01

    Through their dam-building activities and subsequent water storage, beaver have the potential to restore riparian ecosystems and offset some of the predicted effects of climate change by modulating streamflow. Thus, it is not surprising that reintroducing beaver to watersheds from which they have been extirpated is an often-used restoration and climate-adaptation strategy. Identifying sites for reintroduction, however, requires detailed information about habitat factors-information that is not often available at broad spatial scales. Here we explore the potential for beaver relocation throughout the Snohomish River Basin in Washington, USA with a model that identifies some of the basic building blocks of beaver habitat suitability and does so by relying solely on remotely sensed data. More specifically, we developed a generalized intrinsic potential model that draws on remotely sensed measures of stream gradient, stream width, and valley width to identify where beaver could become established if suitable vegetation were to be present. Thus, the model serves as a preliminary screening tool that can be applied over relatively large extents. We applied the model to 5,019 stream km and assessed the ability of the model to correctly predict beaver habitat by surveying for beavers in 352 stream reaches. To further assess the potential for relocation, we assessed land ownership, use, and land cover in the landscape surrounding stream reaches with varying levels of intrinsic potential. Model results showed that 33% of streams had moderate or high intrinsic potential for beaver habitat. We found that no site that was classified as having low intrinsic potential had any sign of beavers and that beaver were absent from nearly three quarters of potentially suitable sites, indicating that there are factors preventing the local population from occupying these areas. Of the riparian areas around streams with high intrinsic potential for beaver, 38% are on public lands and 17% are

  20. Effects of reintroduced beaver (Castor canadensis) on riparian bird community structure along the upper San Pedro River, southeastern Arizona and northern Sonora, Mexico

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    Johnson, Glenn E.; van Riper, Charles

    2014-01-01

    Chapter 1.—We measured bird abundance and richness along the upper San Pedro River in 2005 and 2006, in order to document how beavers (Castor canadensis) may act as ecosystem engineers after their reintroduction to a desert riparian area in the Southwestern United States. In areas where beavers colonized, we found higher bird abundance and richness of bird groups, such as all breeding birds, insectivorous birds, and riparian specialists, and higher relative abundance of many individual species—including several avian species of conservation concern. Chapter 2.—We conducted bird surveys in riparian areas along the upper San Pedro River in southeastern Arizona (United States) and northern Sonora (Mexico) in order to describe factors influencing bird community dynamics and the distribution and abundance of species, particularly those of conservation concern. These surveys were also used to document the effects of the ecosystem-altering activities of a recently reintroduced beavers (Castor canadensis). Chapter 3.—We reviewed Southwestern Willow Flycatcher (Empidonax traillii extimus) nest records and investigated the potential for future breeding along the upper San Pedro River in southeastern Arizona, where in July 2005 we encountered the southernmost verifiable nest attempt for the species. Continued conservation and management of the area’s riparian vegetation and surface water has potential to contribute additional breeding sites for this endangered Willow Flycatcher subspecies. Given the nest record along the upper San Pedro River and the presence of high-density breeding sites to the north, the native cottonwood-willow forests of the upper San Pedro River could become increasingly important to E. t. extimus recovery, especially considering the anticipated effect of the tamarisk leaf beetle (Diorhabda carinulata) on riparian habitat north of the region.

  1. Presence of Antibodies to Leptospira spp. in Black-tailed Prairie Dogs ( Cynomys ludovicianus ) and Beavers ( Castor canadensis ) in Northwestern Mexico.

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    López-Pérez, Andrés M; Carreón-Arroyo, Gerardo; Atilano, Daniel; Vigueras-Galván, Ana L; Valdez, Carlos; Toyos, Daniel; Mendizabal, Daniel; López-Islas, Jonathan; Suzán, Gerardo

    2017-10-01

    Leptospires are widespread spirochete bacteria that infect mammals, including rodents and humans. We investigated the presence of Leptospira antibodies in two species of rodents from San Pedro River Basin (SPRB) in northwestern Mexico as part of the black-tailed prairie dog ( Cynomys ludovicianus ) monitoring plan and the North American beaver ( Castor canadensis ) reintroduction program. We sampled a total of 26 black-tailed prairie dogs and three beavers during October-November 2015. We detected antibodies against Leptospira spp. by microagglutination test in 12 (46%) prairie dogs and in two (67%) beavers. The antibody titers for seropositive rodents varied from 1:100 to 1:200, but none of the animals showed clinical signs of disease. We found seven Leptospira spp. serogroups (Autumnalis, Australis, Bataviae, Canicola, Celledoni, Grippotyphosa, and Sejroe) circulating in rodent species in SPRB. We did not find any differences between sex and age concerning Leptospira-positive rodents. Our findings suggest the presence of endemic cycles and potential risks of Leptospira infection in both species from SPRB. Although the impact of this infection on threatened species remains unclear, human activities and environmental stress might facilitate the emergence or reemergence of leptospirosis disease as has been reported elsewhere.

  2. No evidence for local adaptation to salt stress in the existing populations of invasive Solidago canadensis in China.

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    Li, Junmin; Liu, Haiyan; Yan, Ming; Du, Leshan

    2017-01-01

    Local adaptation is an important mechanism underlying the adaptation of plants to environmental heterogeneity, and the toxicity of salt results in strong selection pressure on salt tolerance in plants and different ecotypes. Solidago canadensis, which is invasive in China, has spread widely and has recently colonized alkali sandy loams with a significant salt content. A common greenhouse experiment was conducted to test the role of local adaptation in the successful invasion of S. canadensis into salty habitats. Salt treatment significantly decreased the growth of S. canadensis, including rates of increase in the number of leaves and plant height; the root, shoot, and total biomass. Furthermore, salt stress significantly reduced the net photosynthetic rate, stomatal conductance, transpiration rate and relative chlorophyll content but significantly increased peroxidase activity and the proline content of S. canadensis and the root/shoot ratio. Two-way analysis of variance showed that salt treatment had a significant effect on the physiological traits of S. canadensis, except for the intercellular CO2 concentration, whereas the population and the salt × population interaction had no significant effect on any physiological traits. Most of the variation in plasticity existed within and not among populations, excep for the root/shoot ratio. S. canadensis populations from soil with moderate/high salt levels grew similarly to S. canadensis populations from soils with low salt levels. No significant correlation between salt tolerance indices and soil salinity levels was observed. The plasticity of the proline content, intercellular CO2 concentration and chlorophyll content had significant correlations with the salt tolerance index. These findings indicate a lack of evidence for local adaption in the existing populations of invasive S. canadensis in China; instead, plasticity might be more important than local adaptation in influencing the physiological traits and salt

  3. No evidence for local adaptation to salt stress in the existing populations of invasive Solidago canadensis in China.

    Directory of Open Access Journals (Sweden)

    Junmin Li

    Full Text Available Local adaptation is an important mechanism underlying the adaptation of plants to environmental heterogeneity, and the toxicity of salt results in strong selection pressure on salt tolerance in plants and different ecotypes. Solidago canadensis, which is invasive in China, has spread widely and has recently colonized alkali sandy loams with a significant salt content. A common greenhouse experiment was conducted to test the role of local adaptation in the successful invasion of S. canadensis into salty habitats. Salt treatment significantly decreased the growth of S. canadensis, including rates of increase in the number of leaves and plant height; the root, shoot, and total biomass. Furthermore, salt stress significantly reduced the net photosynthetic rate, stomatal conductance, transpiration rate and relative chlorophyll content but significantly increased peroxidase activity and the proline content of S. canadensis and the root/shoot ratio. Two-way analysis of variance showed that salt treatment had a significant effect on the physiological traits of S. canadensis, except for the intercellular CO2 concentration, whereas the population and the salt × population interaction had no significant effect on any physiological traits. Most of the variation in plasticity existed within and not among populations, excep for the root/shoot ratio. S. canadensis populations from soil with moderate/high salt levels grew similarly to S. canadensis populations from soils with low salt levels. No significant correlation between salt tolerance indices and soil salinity levels was observed. The plasticity of the proline content, intercellular CO2 concentration and chlorophyll content had significant correlations with the salt tolerance index. These findings indicate a lack of evidence for local adaption in the existing populations of invasive S. canadensis in China; instead, plasticity might be more important than local adaptation in influencing the physiological

  4. Comparative analyses of plastid sequences between native and introduced populations of aquatic weeds Elodea canadensis and E. nuttallii.

    Directory of Open Access Journals (Sweden)

    Tea Huotari

    Full Text Available Non-indigenous species (NIS are species living outside their historic or native range. Invasive NIS often cause severe environmental impacts, and may have large economical and social consequences. Elodea (Hydrocharitaceae is a New World genus with at least five submerged aquatic angiosperm species living in fresh water environments. Our aim was to survey the geographical distribution of cpDNA haplotypes within the native and introduced ranges of invasive aquatic weeds Elodea canadensis and E. nuttallii and to reconstruct the spreading histories of these invasive species. In order to reveal informative chloroplast (cp genome regions for phylogeographic analyses, we compared the plastid sequences of native and introduced individuals of E. canadensis. In total, we found 235 variable sites (186 SNPs, 47 indels and two inversions between the two plastid sequences consisting of 112,193 bp and developed primers flanking the most variable genomic areas. These 29 primer pairs were used to compare the level and pattern of intraspecific variation within E. canadensis to interspecific variation between E. canadensis and E. nuttallii. Nine potentially informative primer pairs were used to analyze the phylogeographic structure of both Elodea species, based on 70 E. canadensis and 25 E. nuttallii individuals covering native and introduced distributions. On the whole, the level of variation between the two Elodea species was 53% higher than that within E. canadensis. In our phylogeographic analysis, only a single haplotype was found in the introduced range in both species. These haplotypes H1 (E. canadensis and A (E. nuttallii were also widespread in the native range, covering the majority of native populations analyzed. Therefore, we were not able to identify either the geographic origin of the introduced populations or test the hypothesis of single versus multiple introductions. The divergence between E. canadensis haplotypes was surprisingly high, and future

  5. Comparative analyses of plastid sequences between native and introduced populations of aquatic weeds Elodea canadensis and E. nuttallii.

    Science.gov (United States)

    Huotari, Tea; Korpelainen, Helena

    2013-01-01

    Non-indigenous species (NIS) are species living outside their historic or native range. Invasive NIS often cause severe environmental impacts, and may have large economical and social consequences. Elodea (Hydrocharitaceae) is a New World genus with at least five submerged aquatic angiosperm species living in fresh water environments. Our aim was to survey the geographical distribution of cpDNA haplotypes within the native and introduced ranges of invasive aquatic weeds Elodea canadensis and E. nuttallii and to reconstruct the spreading histories of these invasive species. In order to reveal informative chloroplast (cp) genome regions for phylogeographic analyses, we compared the plastid sequences of native and introduced individuals of E. canadensis. In total, we found 235 variable sites (186 SNPs, 47 indels and two inversions) between the two plastid sequences consisting of 112,193 bp and developed primers flanking the most variable genomic areas. These 29 primer pairs were used to compare the level and pattern of intraspecific variation within E. canadensis to interspecific variation between E. canadensis and E. nuttallii. Nine potentially informative primer pairs were used to analyze the phylogeographic structure of both Elodea species, based on 70 E. canadensis and 25 E. nuttallii individuals covering native and introduced distributions. On the whole, the level of variation between the two Elodea species was 53% higher than that within E. canadensis. In our phylogeographic analysis, only a single haplotype was found in the introduced range in both species. These haplotypes H1 (E. canadensis) and A (E. nuttallii) were also widespread in the native range, covering the majority of native populations analyzed. Therefore, we were not able to identify either the geographic origin of the introduced populations or test the hypothesis of single versus multiple introductions. The divergence between E. canadensis haplotypes was surprisingly high, and future research may

  6. Development and viability of a translocated beaver Castor fiber population in the Netherlands

    NARCIS (Netherlands)

    Nolet, B.A.; Baveco, J.M.

    1996-01-01

    We monitored survival, reproduction and emigration of a translocated beaver Castor fiber population in the Netherlands for five years and used a stochastic model to assess its viability. Between 1988 and 1991, 42 beavers were released in the Biesbosch National Park. The mortality was initially high

  7. [Patterns of morphological variability in reintroduced populations with two beaver subspecies Castor fiber orientoeuropaeus and Castor fiber belorussicus (Castoridae, Rodentia) as an example].

    Science.gov (United States)

    Korablev, N P; Korablev, P N

    2012-01-01

    Taking as an example two beaver subspecies (Castor fiber orientoeuropaeus and Castor fiber belorussicus) with documented history of population formation, the patterns of morphological variability in translocated groups of mammals are studied. The variability of quantitative and qualitative traits in the formed populations is not characterized by a single direction. The main trend consists in increasing of adaptive norms diversity as related to body size. There observed a slight increase in the level of fluctuating asymmetry, reduction in polymorphism of nonmetric traits, and increase in fraction of rare aberrations. All these may be caused by inbreeding taking place during the period of prapopulations formation. The results of the study allow for considering the intraspecific differentiation as a consequence of adaptive variability (adaptatiogenesis) or subspecies hybridization. As for stochastic processes (genetic drift, founder effect), they seem to not influence the morphological variability significantly. The differences between discrete and dimensional traits are indicative of population groups' peculiarity.

  8. Population genetics and adaptation to climate along elevation gradients in invasive Solidago canadensis.

    Science.gov (United States)

    Moran, Emily V; Reid, Andrea; Levine, Jonathan M

    2017-01-01

    Gene flow between populations may either support local adaptation by supplying genetic variation on which selection may act, or counteract it if maladapted alleles arrive faster than can be purged by selection. Although both such effects have been documented within plant species' native ranges, how the balance of these forces influences local adaptation in invasive plant populations is less clear, in part because introduced species often have lower genetic variation initially but also tend to have good dispersal abilities. To evaluate the extent of gene flow and adaptation to local climate in invasive populations of Solidago canadensis, and the implications of this for range expansion, we compared population differentiation at microsatellite and chloroplast loci for populations across Switzerland and assessed the effect of environmental transfer distance using common gardens. We found that while patterns of differentiation at neutral genetic markers suggested that populations are connected through extensive pollen and seed movement, common-garden plants nonetheless exhibited modest adaptation to local climate conditions. Growth rate and flower production declined with climatic distance from a plant's home site, with clones from colder home sites performing better at or above the range limit. Such adaptation in invasive species is likely to promote further spread, particularly under climate change, as the genotypes positioned near the range edge may be best able to take advantage of lengthening growing seasons to expand the range.

  9. Population genetics and adaptation to climate along elevation gradients in invasive Solidago canadensis.

    Directory of Open Access Journals (Sweden)

    Emily V Moran

    Full Text Available Gene flow between populations may either support local adaptation by supplying genetic variation on which selection may act, or counteract it if maladapted alleles arrive faster than can be purged by selection. Although both such effects have been documented within plant species' native ranges, how the balance of these forces influences local adaptation in invasive plant populations is less clear, in part because introduced species often have lower genetic variation initially but also tend to have good dispersal abilities. To evaluate the extent of gene flow and adaptation to local climate in invasive populations of Solidago canadensis, and the implications of this for range expansion, we compared population differentiation at microsatellite and chloroplast loci for populations across Switzerland and assessed the effect of environmental transfer distance using common gardens. We found that while patterns of differentiation at neutral genetic markers suggested that populations are connected through extensive pollen and seed movement, common-garden plants nonetheless exhibited modest adaptation to local climate conditions. Growth rate and flower production declined with climatic distance from a plant's home site, with clones from colder home sites performing better at or above the range limit. Such adaptation in invasive species is likely to promote further spread, particularly under climate change, as the genotypes positioned near the range edge may be best able to take advantage of lengthening growing seasons to expand the range.

  10. Errors associated with moose-hunter counts of occupied beaver Castor fiber lodges in Norway

    OpenAIRE

    Parker, Howard; Rosell, Frank; Gustavsen, Per Øyvind

    2002-01-01

    In Norway, Sweden and Finland moose Alces alces hunting teams are often employed to survey occupied beaver (Castor fiber and C. canadensis) lodges while hunting. Results may be used to estimate population density or trend, or for issuing harvest permits. Despite the method's increasing popularity, the errors involved have never been identified. In this study we 1) compare hunting-team counts of occupied lodges with total counts, 2) identify the sources of error between counts and 3) evaluate ...

  11. Comparative Metagenomics of Cellulose- and Poplar Hydrolysate-Degrading Microcosms from Gut Microflora of the Canadian Beaver (Castor canadensis and North American Moose (Alces americanus after Long-Term Enrichment

    Directory of Open Access Journals (Sweden)

    Mabel T. Wong

    2017-12-01

    Full Text Available To identify carbohydrate-active enzymes (CAZymes that might be particularly relevant for wood fiber processing, we performed a comparative metagenomic analysis of digestive systems from Canadian beaver (Castor canadensis and North American moose (Alces americanus following 3 years of enrichment on either microcrystalline cellulose or poplar hydrolysate. In total, 9,386 genes encoding CAZymes and carbohydrate-binding modules (CBMs were identified, with up to half predicted to originate from Firmicutes, Bacteroidetes, Chloroflexi, and Proteobacteria phyla, and up to 17% from unknown phyla. Both PCA and hierarchical cluster analysis distinguished the annotated glycoside hydrolase (GH distributions identified herein, from those previously reported for grass-feeding mammals and herbivorous foragers. The CAZyme profile of moose rumen enrichments also differed from a recently reported moose rumen metagenome, most notably by the absence of GH13-appended dockerins. Consistent with substrate-driven convergence, CAZyme profiles from both poplar hydrolysate-fed cultures differed from cellulose-fed cultures, most notably by increased numbers of unique sequences belonging to families GH3, GH5, GH43, GH53, and CE1. Moreover, pairwise comparisons of moose rumen enrichments further revealed higher counts of GH127 and CE15 families in cultures fed with poplar hydrolysate. To expand our scope to lesser known carbohydrate-active proteins, we identified and compared multi-domain proteins comprising both a CBM and domain of unknown function (DUF as well as proteins with unknown function within the 416 predicted polysaccharide utilization loci (PULs. Interestingly, DUF362, identified in iron–sulfur proteins, was consistently appended to CBM9; on the other hand, proteins with unknown function from PULs shared little identity unless from identical PULs. Overall, this study sheds new light on the lignocellulose degrading capabilities of microbes originating from

  12. Genetic diversity and population structure of castor (Ricinus communis L.) germplasm within the U.S. collection assessed with EST-SSR markers

    Science.gov (United States)

    Castor is an important oilseed crop and although its oil is inedible, it has multiple industrial and pharmaceutical applications. The entire U.S. castor germplasm collection was previously screened for oil content and fatty acid composition, but its genetic diversity and population structure has not...

  13. Phylogeographic and population genetic structure of bighorn sheep ( Ovis canadensis ) in North American deserts.

    Science.gov (United States)

    Buchalski, Michael R; Sacks, Benjamin N; Gille, Daphne A; Penedo, Maria Cecilia T; Ernest, Holly B; Morrison, Scott A; Boyce, Walter M

    2016-06-09

    Fossil data are ambiguous regarding the evolutionary origin of contemporary desert bighorn sheep ( Ovis canadensis subspecies). To address this uncertainty, we conducted phylogeographic and population genetic analyses on bighorn sheep subspecies found in southwestern North America. We analyzed 515 base pairs of mtDNA control region sequence and 39 microsatellites in 804 individuals from 58 locations. Phylogenetic analyses revealed 2 highly divergent clades concordant with Sierra Nevada ( O. c. sierrae ) and Rocky Mountain ( O. c. canadensis ) bighorn and showed that these 2 subspecies both diverged from desert bighorn prior to or during the Illinoian glaciation (~315-94 thousand years ago [kya]). Desert bighorn comprised several more recently diverged haplogroups concordant with the putative Nelson ( O. c. nelsoni ), Mexican ( O. c. mexicana ), and Peninsular ( O. c. cremnobates ) subspecies. Corresponding estimates of effective splitting times (~17-3 kya), and haplogroup ages (~85-72 kya) placed the most likely timeframe for divergence among desert bighorn subspecies somewhere within the last glacial maximum. Median-joining haplotype network and Bayesian skyline analyses both indicated that desert bighorn collectively comprised a historically large and haplotype-diverse population, which subsequently lost much of its diversity through demographic decline. Using microsatellite data, discriminant analysis of principle components (DAPC) and Bayesian clustering analyses both indicated genetic structure concordant with the geographic distribution of 3 desert subspecies. Likewise, microsatellite and mitochondrial-based F ST comparisons revealed significant fixation indices among the desert bighorn genetic clusters. We conclude these desert subspecies represent ancient lineages likely descended from separate Pleistocene refugial populations and should therefore be managed as distinct taxa to preserve maximal biodiversity. Los datos de fósiles sobre el origen evolutivo

  14. A study of gizzard nematodes and renal coccidiosis in Canada geese (Branta canadensis interior) of the Mississippi Valley population

    Science.gov (United States)

    Tuggle, Benjamin N.

    1982-01-01

    A total of 309 Mississippi Valley Population Canada geese, Branta canadensis interior, of different sex and age groups was collected from three locations in the Mississippi Flyway from 1979-1981 and examined for gizzard nematodes and renal coccidia. Three species of nematodes were removed from the gizzards, Amidostomum anseris, A. spatulatum, and Epomidiostomum crami. The latter two species are reported from this population of geese for the first time. Gizzard nematodes were found in 95.2% of all Canada geese examined, with A. anseris being the most abundant of the three species. There was no statistically significant difference between immatures and adults in the abundance of total nematodes species however, immature geese carried significantly more A. anseris and adult geese harbored significantly more A. spatulatum and E. crami infections. No significant difference in gizzard worm infections between male and female birds was observed. The abundance of overall gizzard nematodes was greatest in Canada geese from Winisk, Ontario (11.9), but the abundance of worms in southern Illinois geese (10.0) was similar. Geese from Horicon National Wildlife Refuge had the lowest abundance of infection, 7.5. The overall abundance of nematodes showed a general increase the second year of the study in each sex and age group and at each collection area. Each of three species of nematodes was responsible for some degree of damage to the gizzard lining and koilin, but E. crami was the most pathogenic of the species recovered. The occurrence of renal coccidiosis in Canada geese of this flyway is reported for the first time; the etiologic agent is Eimeria clarkei. The oocysts and/or endogenous stages of E. clarkei were present in 6.8% of the Canada geese sampled and this was the only species found. Male and female geese showed no significant differences in E. clarkei infections, however, significantly more immature geese than adult geese were infected with this species. A cell

  15. Identifying source populations for the reintroduction of the Eurasian beaver, Castor fiber L. 1758, into Britain: evidence from ancient DNA.

    Science.gov (United States)

    Marr, Melissa M; Brace, Selina; Schreve, Danielle C; Barnes, Ian

    2018-02-09

    Establishing true phylogenetic relationships between populations is a critical consideration when sourcing individuals for translocation. This presents huge difficulties with threatened and endangered species that have become extirpated from large areas of their former range. We utilise ancient DNA (aDNA) to reconstruct the phylogenetic relationships of a keystone species which has become extinct in Britain, the Eurasian beaver Castor fiber. We sequenced seventeen 492 bp partial tRNAPro and control region sequences from Late Pleistocene and Holocene age beavers and included these in network, demographic and genealogy analyses. The mode of postglacial population expansion from refugia was investigated by employing tests of neutrality and a pairwise mismatch distribution analysis. We found evidence of a pre-Late Glacial Maximum ancestor for the Western C. fiber clade which experienced a rapid demographic expansion during the terminal Pleistocene to early Holocene period. Ancient British beavers were found to originate from the Western phylogroup but showed no phylogenetic affinity to any one modern relict population over another. Instead, we find that they formed part of a large, continuous, pan-Western European clade that harbored little internal substructure. Our study highlights the utility of aDNA in reconstructing population histories of extirpated species which has real-world implications for conservation planning.

  16. Population size of Cuban Parrots Amazona leucocephala and Sandhill Cranes Grus canadensis and community involvement in their conservation in northern Isla de la Juventud, Cuba

    Science.gov (United States)

    Aguilera, X.G.; Alvarez, V.B.; Wiley, J.W.; Rosales, J.R.

    1999-01-01

    The Cuban Sandhill Crane Grus canadensis nesiotes and Cuban Parrot Amazona leucocephala palmarum are considered endangered species in Cuba and the Isla de la Juventud (formerly Isla de Pinos). Coincident with a public education campaign, a population survey for these species was conducted in the northern part of the Isla de la Juventud on 17 December 1995, from 06hoo to 10hoo. Residents from throughout the island participated, manning 98 stations, with 1-4 observers per station. Parrots were observed at 60 (61.2%) of the stations with a total of 1320, maximum (without correction for duplicate observations), and 1100, minimum (corrected), individuals counted. Sandhill cranes were sighted at 38 (38.8%) of the stations, with a total of 115 individuals. Cranes and parrots co-occurred at 20 (20.4%) of the stations.

  17. Assessment of an Impact of Mechanical Regulation on Selected Morphometric and Productive Parameters of Invasive Species Solidago Canadensis Population in Agricultural Land

    Directory of Open Access Journals (Sweden)

    Končeková Lýdia

    2015-12-01

    Full Text Available Repeated mowing is considered as one of the effective control methods against species of the genus Solidago. This paper evaluates the impact of the repeated mowing on selected morphometric and productive characteristics of the invasive neophyte Solidago canadensis in the district of Rimavská Sobota in Central Slovakia. Permanent research plots (PRPs were established within anthropogenic habitat on an abandoned land that was divided into two variants. In the first variant, the mechanical regulation - mowing was applied. The second variant was without the regulation. The mechanical regulation of the populations was carried out in June and August during the growing season 2011. The results showed that the mechanical regulation did not have a clear impact on the population density. The decreasing trend of the number of shoots within the mowed variant was found only in one research plot (PRP3. The other plots showed an increase in the number of individuals by 2.7 and 32.7% between the mowings. Statistically highly significant differences in terms of the mowing impact on the height of the individuals were found in all PRPs. The difference in the weight of dry aboveground biomass between the mowings was 221.87 g, which represents 36.41%. Double the difference (48.8% was recorded in the dry weight of the underground biomass in the regulated stand compared with the unregulated stand (165.1 and 322.5 g/m2, respectively. Although there was a short-term success achieved by the application of the two mowings during the growing period, the pursued objective was not reached.

  18. Beaver-mediated methane emission: The effects of population growth in Eurasia and the Americas.

    Science.gov (United States)

    Whitfield, Colin J; Baulch, Helen M; Chun, Kwok P; Westbrook, Cherie J

    2015-02-01

    Globally, greenhouse gas budgets are dominated by natural sources, and aquatic ecosystems are a prominent source of methane (CH(4)) to the atmosphere. Beaver (Castor canadensis and Castor fiber) populations have experienced human-driven change, and CH(4) emissions associated with their habitat remain uncertain. This study reports the effect of near extinction and recovery of beavers globally on aquatic CH4 emissions and habitat. Resurgence of native beaver populations and their introduction in other regions accounts for emission of 0.18-0.80 Tg CH(4) year(-1) (year 2000). This flux is approximately 200 times larger than emissions from the same systems (ponds and flowing waters that became ponds) circa 1900. Beaver population recovery was estimated to have led to the creation of 9500-42 000 km(2) of ponded water, and increased riparian interface length of >200 000 km. Continued range expansion and population growth in South America and Europe could further increase CH(4) emissions.

  19. CASTOR end-to-end monitoring

    International Nuclear Information System (INIS)

    Rekatsinas, Theodoros; Duellmann, Dirk; Pokorski, Witold; Ponce, Sebastien; Rabacal, Bartolomeu; Waldron, Dennis; Wojcieszuk, Jacek

    2010-01-01

    With the start of Large Hadron Collider approaching, storage and management of raw event data, as well as reconstruction and analysis data, is of crucial importance for the researchers. The CERN Advanced STORage system (CASTOR) is a hierarchical system developed at CERN, used to store physics production files and user files. CASTOR, as one of the essential software tools used by the LHC experiments, has to provide reliable services for storing and managing data. Monitoring of this complicated system is mandatory in order to assure its stable operation and improve its future performance. This paper presents the new monitoring system of CASTOR which provides operation and user request specific metrics. This system is build around a dedicated, optimized database schema. The schema is populated by PL/SQL procedures, which process a stream of incoming raw metadata from different CASTOR components, initially collected by the Distributed Logging Facility (DLF). A web interface has been developed for the visualization of the monitoring data. The different histograms and plots are created using PHP scripts which query the monitoring database.

  20. The CASTOR initiative

    Energy Technology Data Exchange (ETDEWEB)

    Le Thiez, Pierre [GEOGREEN (France)

    2008-07-15

    In this paper it is spoken over the CASTOR project, which has 31 partners of 12 European countries. Also it is spoken on the strategy for the CO{sub 2} reduction in Europe; what on the capture and post-combustion has been done, and what as far as geologic CO{sub 2} storage has been accomplished. [Spanish] En esta ponencia se habla sobre el proyecto Castor, el cual cuenta con 31 socios de 12 paises europeos. Tambien se habla sobre la estrategia para la reduccion de CO{sub 2} en Europa; lo que se ha hecho en cuanto a captura post-combustion y lo que se ha hecho para el almacenamiento geologico de CO{sub 2}.

  1. The CASTOR shower library

    International Nuclear Information System (INIS)

    Mundim Filho, Luiz Martins; Carvalho, Wagner de Paula

    2012-01-01

    Full text: CASTOR (Centaur And Strange Object Research) is an electromagnetic (EM) and hadronic (HAD) calorimeter, based on tungsten and quartz plates, operating in the CMS Detector (Compact Muon Solenoid) at LHC. The calorimeter detects Cerenkov radiation and is positioned around the beam pipe in the very forward region of CMS (at 14.38 m from the interaction point), covering the pseudo-rapidity range between -6.6 ≤ η≤-5.1 . It is longitudinally segmented into 14 sections, 2 for the EM and 12 for the HAD parts and is 16-fold azimuthally symmetric around the beam pipe. A Shower Library is needed for CASTOR Monte Carlo simulation, as the full simulation of showers takes a long time and the high multiplicity of particles in the forward region makes this simulation very time consuming. The Shower Library is used as a look-up table in the form of a ROOT file, so that when a simulated particle enters the detector with a certain energy and direction, characterized by the azimuthal angle φ and the pseudo-rapidity η, instead of making the full simulation of the shower in CASTOR, it is substituted by one already stored in the Shower Library. Showers corresponding to two types of particles are included in the Shower Library: electrons (or photons) and charged pions. The software implemented to make the Shower Library is described, as well as the validation of this library and timing studies. This package has been developed in the context of the official software of the CMS Collaboration, CMSSW. (author)

  2. Effect of plant and row spacing on the yield and oil contents of castor ...

    African Journals Online (AJOL)

    Castor (Ricinus communis L.) is an industrial non edible oilseed adapted to drier areas. An experiment was conducted in the Central Rift Valley of Ethiopia to determine optimum plant population of castor variety Hiruy. Four plant (50, 60, 70 and 80 cm) and four row spacing (60,80,100 and 120 cm) were arranged in factorial ...

  3. Induced mutations in castor

    International Nuclear Information System (INIS)

    Ganesan, K.; Javad Hussain, H.S.; Vindhiyavarman, P.

    2001-01-01

    Castor (Ricinus communis L.) is an important oilseed crop in India. To create variability mutations were induced in two cultivars 'TMV5' (maturing in 130-140 days) and 'CO1' (perennial type). Gamma rays and diethyl sulphate and ethidium bromide were used for seed treatment. Ten doses, from 100 to 1000 Gy were employed. For chemical mutagenesis five concentrations of mutagenes from 10 to 50 mM were tried. No economic mutants could be isolated after treatment with the chemical mutagens. The following economic mutants were identified in the dose 300 Gy of gamma rays. Annual types from perennial CO 1 castor CO 1 is a perennial variety (8-10 years) with bold seeds (100 seed weight 90 g) and high oil content (57%). Twenty-one lines were isolated with annual types (160-180 days) with high yield potential as well as bold seeds and high oil content. These mutants, identified in M 3 generation were bred true in subsequent generations up to M 8 generation. Critical evaluation of the mutants in yield evaluation trials is in progress

  4. Constituents of the essential oil in Solidago canadensis L. from Eurasia

    OpenAIRE

    Olga Shelepova; Yulia Vinogradova; Boris Zaitchik; Alexander Ruzhitsky; Olga Grygorieva; Ján Brindza

    2018-01-01

    Hydro distilled essential oils in air-dry samples of aerial parts of Solidago canadensis L., (Asteraceae) from eight local invasive populations were investigated by GC-MS analysis. A comparative study on quantity and composition of the essential oils obtained from plants, growing in different ecological and climatic conditions, ontogenesis phase and different plant organs was carried out The major compounds detected in oil samples of S. canadensis were α-pinene (1.3 - 61.27%), limonene ...

  5. 21 CFR 172.876 - Castor oil.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Castor oil. 172.876 Section 172.876 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... Multipurpose Additives § 172.876 Castor oil. The food additive castor oil may be safely used in accordance with...

  6. LHCD experiments on tokamak CASTOR

    International Nuclear Information System (INIS)

    Zacek, F.; Badalec, J.; Jakubka, J.; Kryska, L.; Preinhaelter, J.; Stoeckel, J.; Valovic, M.; Nanobashvili, S.; Weixelbaum, L.; Wenzel, U.; Spineanu, F.; Vlad, M.

    1990-10-01

    A short survey is given of the experimental activities at the small Prague tokamak CASTOR. They concern primarily the LH current drive using multijunction waveguide grills as launching antennae. During two last years the, efforts were focused on a study of the electrostatic and magnetic fluctuations under conditions of combined inductive/LHCD regimes and of the relation of the level of these fluctuations to the anomalous particles transport in tokamak CASTOR. Results of the study are discussed in some detail. (author). 24 figs., 51 refs

  7. Intraspecific variation in Tsuga canadensis foliar chemistry

    Science.gov (United States)

    Laura Ingwell; Joseph Brady; Matthew Fitzpatrick; Brian Maynard; Richard Casagrande; Evan Preisser

    2009-01-01

    Three groups of eastern hemlock (Tsuga canadensis Carr.) trees were analyzed to compare their chemical composition and the potential for naturally occurring resistance to hemlock woolly adelgid (Adelges tsguae...

  8. Labelling of castor oil for myocardial study

    International Nuclear Information System (INIS)

    Hallaba, E.; Al-Suhybani, A.; Zaki, F.S.; Abdullah, M.E.

    1985-01-01

    The labelling of castor oil, hydrolyzed castor oil and oleic acid by iodine monochloride and chloramine-T was investigated. The effect of iodinating agent and concentration of castor oil on labelling yield was studied. A comparative pharmacological study with analog aliphatic acids was carried out. Castor oil labelled with iodine monochloride concentrates in heart and liver in good proportion, better than other natural fatty acids and nearly equal to analog fatty acids. Infrared study revealed that the OH group in ricinoleic acid may protect the sup(125)I added across the double bond with minor changes in biochemical properties causing better extraction by muscle of the heart. (author)

  9. Castor Oil-Based Biodegradable Polyesters.

    Science.gov (United States)

    Kunduru, Konda Reddy; Basu, Arijit; Haim Zada, Moran; Domb, Abraham J

    2015-09-14

    This Review compiles the synthesis, physical properties, and biomedical applications for the polyesters based on castor oil and ricinoleic acid. Castor oil has been known for its medicinal value since ancient times. It contains ∼90% ricinoleic acid, which enables direct chemical transformation into polyesters without interference of other fatty acids. The presence of ricinoleic acid (hydroxyl containing fatty acid) enables synthesis of various polyester/anhydrides. In addition, castor oil contains a cis-double bond that can be hydrogenated, oxidized, halogenated, and polymerized. Castor oil is obtained pure in large quantities from natural sources; it is safe and biocompatible.

  10. Synthesis of novel castor oil phosphonates

    Science.gov (United States)

    Castor oil has served as a versatile hydroxy fatty acid (HFA); its principle component, ricinoleic acid, can be isolated from castor oil and has been modified extensively for a number of applications. Additionally, phosphonates and their corresponding phosphonic acids are a functional moiety that ha...

  11. Weed management strategies for castor bean crops

    Directory of Open Access Journals (Sweden)

    Augusto Guerreiro Fontoura Costa

    2014-04-01

    Full Text Available Castor bean crops are agriculturally relevant due to the quality and versatility of their oil, both for the chemical industry and for biodiesel production. Proper weed management is important for both the cultivation and the yield of castor bean crops; therefore, the intention of the present work is to review pertinent information regarding weed management, including the studies regarding weed interference periods, chemical controls for use in different crop production systems and herbicide selectivity, for castor bean crops. Weed science research for castor bean crops is scarce. One of the main weed management challenges for castor bean crops is the absence of herbicides registered with the Ministry of Agriculture, Livestock and Food Supply (MALFS. Research for viable herbicides for weed control in castor bean crops should be directed by research and/or rural extension institutions, associations and farmers cooperatives, as well as by manufactures, for the registration of these selective herbicides, which would be primarily used to control eudicotyledons in castor bean crops. New studies involving the integration of weed control methods in castor bean also may increase the efficiency of weed management, for both small farmers using traditional crop methods in the Brazilian Northeast region, as well as for areas with the potential for large scale production, using conservation tillage systems, such as the no-tillage crop production system.

  12. Labelling of castor oil for myocardial studies

    International Nuclear Information System (INIS)

    Hallaba, E.; Al-Suhybani, A.; Zaki, F.S.; Abdullah, M.E.

    1985-01-01

    The labelling of castor oil, hydrolysed castor oil and oleic acid was investigated by the iodine monochloride and chloramine-T methods. The effect of the iodinating agent and the concentration of castor oil on the labelling yield was studied. A comparative pharmacological study with analog aliphatic fatty acids was carried out. Castor oil labelled with iodine monochloride concentrated in the heart and liver in good proportion, better than other natural fatty acids and nearly equal to analog aliphatic fatty acids. An infra-red study showed that the OH group of the ricinoleic acid apparently protects the 125 I added on the double bond, with minor changes in biochemical properties and better uptake by the heart muscle. (author)

  13. Castor (Ricinus communis L. and Pigweed (Amaranthus retroflexus L. Growth Indices in Terms of Interference

    Directory of Open Access Journals (Sweden)

    naser jafarzadeh

    2016-11-01

    Full Text Available Introduction Growth analysis has been widely used in breeding programs to identify the important plant developmental phases and components related to higher yield under a particular set of environmental conditions. Castor bean (Ricinus communis L. is an important commercial crop. Castor oil based by products is used in manufacturing of several commercially important commodities like surfactants, coatings, greases, pharmaceuticals, cosmetics, polyesters, polymers, etc. Interference (Interactive effects among species on inter-species populations is one of the main issues on the eco-physiology of plant populations where weeds impose negative effects by approaching the plant to compete in light, water and nutrient elements availability and results in reduced growth and yield (Shinggu et al., 2011. Growth indices are useful for interpreting plant reactions to the crop and weed density. Various reasons have been attributed for the low productivity among the most important is weed competition (Radosevich, 1987. The aim of the present experiment was evaluating the interference effects of redroot pigweed on growth indices of castor bean in northwest of Iran. Materials and methods This experiment was conducted in Urmia, Iran (Agricultural Research of West Azarbayjan, Saatlo Station (37°44´18״ N Latitude and 45° 10´ 53״ E Longitude, at 1338 m above sea level in 2012. The soil of the experimental field was sandy - loam, with pH of 7.2. Competitive pattern of experiment was in two-factor based on a randomized complete block design (RCBD with three replications arranged in four castor plant densities (3, 4, 5 and 6 plants.m-2 and four redroot pigweed densities (0, 5, 10 and 15 plants.m-2. Redroot pigweed and castor seeds were simultaneously directly planted on the 22th May in 2012. Redroot pigweed plants were weeded at the times related to the treatments level. Irrigation and intercultural operations were performed whenever necessary. Plots were 3m×5m

  14. Outbreaks of Chrysodeixis includens (Walker (Lepidoptera: Noctuidae in common bean and castor bean in São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Edson Luiz Lopes Baldin

    2014-12-01

    Full Text Available Since 2009, increasing populations of Chrysodeixis includens (Walker (Lepidoptera: Noctuidae have been observed in cultivated common bean (Phaseolus vulgaris L. and castor bean (Ricinus communis L. at the Lageado Experimental Farm, belonging to the FCA/UNESP, Botucatu, São Paulo State, Brazil. Defoliations around 80% and 50% were observed in the common bean cv. Pérola and castor bean cv. IAC-2028, respectively. Samples of individuals (caterpillars and pupae were collected in the field, and kept in laboratory until adult emergence aiming to confirm the species. These are new observations for common bean in São Paulo State and, in the case of castor bean, unpublished in Brazil. It suggests that C. includens has adapted to attack other agricultural crops, demanding attention of common bean and castor bean producers.

  15. VARIATIONS IN SEED TRAITS OF CASTOR (Ricinus communis ...

    African Journals Online (AJOL)

    The fatty acid composition of Malaysian castor seed oil reveals that ... ricinoleic acid content from India and Brazil castor bean oils were ... Standard cultural practices were applied to all plots. .... will influence the utilization of other nutrients by.

  16. Comeback of the beaver Castor fiber: An overview of old and new conservation problems

    NARCIS (Netherlands)

    Nolet, B.A.; Rosell, F.

    1998-01-01

    Due to over-hunting c. 1200 Eurasian beavers Castor fiber survived in eight relict populations in Europe and Asia at the beginning of the 20th century. Following hunting restrictions and translocation programmes in IS countries, the Eurasian beaver became re-established over much of its former

  17. 21 CFR 178.3280 - Castor oil, hydrogenated.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Castor oil, hydrogenated. 178.3280 Section 178.3280 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD... Certain Adjuvants and Production Aids § 178.3280 Castor oil, hydrogenated. Hydrogenated castor oil may be...

  18. 75 FR 40751 - Castor Oil, Ethoxylated, Oleate; Tolerance Exemption

    Science.gov (United States)

    2010-07-14

    ... ENVIRONMENTAL PROTECTION AGENCY 40 CFR Part 180 [EPA-HQ-OPP-2010-0231; FRL-8834-4] Castor Oil... castor oil, ethoxylated, oleate (CAS Reg. No. 220037-02-5) with a minimum number average molecular weight... regulation eliminates the need to establish a maximum permissible level for residues of castor oil...

  19. Chromosomal abnormalities in roots of aquatic plant Elodea canadensis as a tool for testing genotoxicity of bottom sediments.

    Science.gov (United States)

    Zotina, Tatiana; Medvedeva, Marina; Trofimova, Elena; Alexandrova, Yuliyana; Dementyev, Dmitry; Bolsunovsky, Alexander

    2015-12-01

    Submersed freshwater macrophytes are considered as relevant indicators for use in bulk bottom sediment contact tests. The purpose of this study was to estimate the validity of endpoints of aquatic plant Elodea canadensis for laboratory genotoxicity testing of natural bottom sediments. The inherent level of chromosome abnormalities (on artificial sediments) in roots of E. canadensis under laboratory conditions was lower than the percentage of abnormal cells in bulk sediments from the Yenisei River. The percentage of abnormal cells in roots of E. canadensis was more sensitive to the presence of genotoxic agents in laboratory contact tests than in the natural population of the plant. The spectra of chromosomal abnormalities that occur in roots of E. canadensis under natural conditions in the Yenisei River and in laboratory contact tests on the bulk bottom sediments from the Yenisei River were similar. Hence, chromosome abnormalities in roots of E. canadensis can be used as a relevant and sensitive genotoxicity endpoint in bottom sediment-contact tests. Copyright © 2015 Elsevier Inc. All rights reserved.

  20. Leaf area prediction models for Tsuga canadensis in Maine

    Science.gov (United States)

    Laura S. Kenefic; R.S. Seymour

    1999-01-01

    Tsuga canadensis (L.) Carr. (eastern hemlock) is a common species throughout the Acadian forest. Studies of leaf area and growth efficiency in this forest type have been limited by the lack of equations to predict leaf area of this species. We found that sapwood area was an effective leaf area surrogate in T. canadensis, though...

  1. 2003 Castor seminar - 120 tons of high technology for safety

    International Nuclear Information System (INIS)

    Anon.

    2003-01-01

    Castor (Cask for Storage and Transport of Radioactive Material) has become a symbol of the pros and cons of nuclear power in the public debate. Actually, what is Castor, and what is the technology behind this name? Siempelkamp Nukleartechnik and the Gesellschaft fuer Nuklearbehaelter mbH (GNB) invited interested parties to a presentation of the concept, technology, and manufacture on the occasion of the 2003 Castor Seminar. There was the possibility, among other events, to attend the casting process of a Castor cask at the Krefeld foundry of Siempelkamp. GNB offered a visit to its Muelheim works where the final assembly of the Castor cask could be observed. (orig.)

  2. epi-Cubebanes from Solidago canadensis.

    Science.gov (United States)

    Kasali, Adeleke A; Ekundayo, Olusegun; Paul, Claudia; König, Wilfried A

    2002-04-01

    GC-MS of the essential oil prepared by hydrodistillation of the green parts of a specimen of Solidago canadensis collected near Katowice, Poland, revealed two new sesquiterpene hydrocarbons. Their EI mass spectra resembled the mass spectrum of beta-ylangene (1) but the retention indices of the new compounds differed markedly from this known compound. After isolation of the new compounds by preparative GC their investigation by one- and two-dimensional NMR techniques resulted in the identification of 6-epi-alpha-cubebene (2) (minor constituent, 1.5%) and 6-epi-beta-cubebene (3) (major constituent, 20.5%).

  3. Nalaz tombusvirusa na vrsti Erigeron canadensis L.

    OpenAIRE

    Jeknić, Zoran; Erić, Živojin; Grbelja, Julijana

    1988-01-01

    Iz listova zaraženih primjeraka korovne biljke Erigeron canadensis L. koji su rasli na nekoliko lokaliteta u Sarajevu izoliran je virus iz skupine tombusvirusi. Identifikacija virusa izvršena je na osnovi reakcije pokusnih biljaka, analize ultratankih presjeka kroz zaraženo tkivo, morfologije i veličine virusnih čestica, te na osnovi seroloških reakcija metodom dvostruke imunodifuzije u agarskom gelu i čvrsto fazne imunoelektronske mikroskopije (SPIEM). U serološkim pokusima upotrijebljen je ...

  4. Role of carriers in the transmission of pneumonia in bighorn sheep (Ovis canadensis)

    OpenAIRE

    Raghavan, Bindu; Erickson, Kayla; Kugadas, Abirami; Batra, Sai A.; Call, Douglas R.; Davis, Margaret A.; Foreyt, William J.; Srikumaran, Subramaniam

    2016-01-01

    ABSTRACT In the absence of livestock contact, recurring lamb mortality in bighorn sheep (Ovis canadensis) populations previously exposed to pneumonia indicates the likely presence of carriers of pneumonia-causing pathogens, and possibly inadequate maternally derived immunity. To investigate this problem we commingled na?ve, pregnant ewes (n=3) with previously exposed rams (n=2). Post-commingling, all ewes and lambs born to them acquired pneumonia-causing pathogens (leukotoxin-producing Pasteu...

  5. Tape write-efficiency improvements in CASTOR

    International Nuclear Information System (INIS)

    Murray, S; Bahyl, V; Cancio, G; Cano, E; Lo Presti, G; Lo Re, G; Ponce, S; Kotlyar, V

    2012-01-01

    The CERN Advanced STORage manager (CASTOR) is used to archive to tape the physics data of past and present physics experiments. For reasons of physical storage space, all of the tape resident data in CASTOR are repacked onto higher density tapes approximately every two years. Improving the performance of writing files smaller than 2GB to tape is essential in order to keep the time needed to repack all of the tape resident data within a period of no more than 1 year. This paper reports on the solution to writing efficiently to tape that is currently in its early deployment phases at CERN.

  6. Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.

    Directory of Open Access Journals (Sweden)

    Yang Jun-Bo

    2010-12-01

    Full Text Available Abstract Background The castor bean (Ricinus communis L., a monotypic species in the spurge family (Euphorbiaceae, 2n = 20, is an important non-edible oilseed crop widely cultivated in tropical, sub-tropical and temperate countries for its high economic value. Because of the high level of ricinoleic acid (over 85% in its seed oil, the castor bean seed derivatives are often used in aviation oil, lubricants, nylon, dyes, inks, soaps, adhesive and biodiesel. Due to lack of efficient molecular markers, little is known about the population genetic diversity and the genetic relationships among castor bean germplasm. Efficient and robust molecular markers are increasingly needed for breeding and improving varieties in castor bean. The advent of modern genomics has produced large amounts of publicly available DNA sequence data. In particular, expressed sequence tags (ESTs provide valuable resources to develop gene-associated SSR markers. Results In total, 18,928 publicly available non-redundant castor bean EST sequences, representing approximately 17.03 Mb, were evaluated and 7732 SSR sites in 5,122 ESTs were identified by data mining. Castor bean exhibited considerably high frequency of EST-SSRs. We developed and characterized 118 polymorphic EST-SSR markers from 379 primer pairs flanking repeats by screening 24 castor bean samples collected from different countries. A total of 350 alleles were identified from 118 polymorphic SSR loci, ranging from 2-6 per locus (A with an average of 2.97. The EST-SSR markers developed displayed moderate gene diversity (He with an average of 0.41. Genetic relationships among 24 germplasms were investigated using the genotypes of 350 alleles, showing geographic pattern of genotypes across genetic diversity centers of castor bean. Conclusion Castor bean EST sequences exhibited considerably high frequency of SSR sites, and were rich resources for developing EST-SSR markers. These EST-SSR markers would be particularly

  7. Individual Plasticity of the Shade Response of the Invasive Solidago canadensis in China.

    Directory of Open Access Journals (Sweden)

    Leshan Du

    Full Text Available To evaluate the population variation, individual plasticity, and local adaptability of Solidago canadensis in response to shade treatment, we conducted a common pots experiment with a total of 150 ramets (5 genets, 15 populations, and 2 treatments subjected to both control (natural light and shady treatment (10% of natural light. Shade treatment significantly reduced growth and content of defense metabolites in S. canadensis. Compared to control, shading led to increased height, decreased basal diameter, increased leaf width, increased leaf length, increased chlorophyll content, stronger photosynthetic rate (Pn, stronger stomatal conductance (gs, and lower root to shoot ratio. Three-way analysis of variance revealed geographical origin to significantly affect the basal diameter of S. canadensis, while genotype significantly affected plant height, intercelluar CO2 concentration (Ci, transpiration rate (Tr, and proline content. Significant interactive effects between shade and geographic origin were prevalent for most traits. The phenotypic differentiation coefficient of the plasticity of all traits was below 0.4, indicating that most of all variations can be found among individuals within populations. Phenotypic selection analysis revealed that fitness was significantly positively related to plant height, basal diameter, Ci, total flavonoid content, as well as the plasticity of plant height, leaf length, leaf width, gs, Ci, total flavonoid content, and malondialdehyde content under the control condition. However, subjected to shade, fitness was only significantly positively related to plant height, basal diameter, and the plasticity of basal diameter. Rather than local adaption, these results suggest that individual plasticity played a more prominent role in the shade response of the invasive S. canadensis.

  8. Individual Plasticity of the Shade Response of the Invasive Solidago canadensis in China.

    Science.gov (United States)

    Du, Leshan; Liu, Haiyan; Yan, Ming; Li, Junmin; Li, Junsheng

    2017-01-01

    To evaluate the population variation, individual plasticity, and local adaptability of Solidago canadensis in response to shade treatment, we conducted a common pots experiment with a total of 150 ramets (5 genets, 15 populations, and 2 treatments) subjected to both control (natural light) and shady treatment (10% of natural light). Shade treatment significantly reduced growth and content of defense metabolites in S. canadensis. Compared to control, shading led to increased height, decreased basal diameter, increased leaf width, increased leaf length, increased chlorophyll content, stronger photosynthetic rate (Pn), stronger stomatal conductance (gs), and lower root to shoot ratio. Three-way analysis of variance revealed geographical origin to significantly affect the basal diameter of S. canadensis, while genotype significantly affected plant height, intercelluar CO2 concentration (Ci), transpiration rate (Tr), and proline content. Significant interactive effects between shade and geographic origin were prevalent for most traits. The phenotypic differentiation coefficient of the plasticity of all traits was below 0.4, indicating that most of all variations can be found among individuals within populations. Phenotypic selection analysis revealed that fitness was significantly positively related to plant height, basal diameter, Ci, total flavonoid content, as well as the plasticity of plant height, leaf length, leaf width, gs, Ci, total flavonoid content, and malondialdehyde content under the control condition. However, subjected to shade, fitness was only significantly positively related to plant height, basal diameter, and the plasticity of basal diameter. Rather than local adaption, these results suggest that individual plasticity played a more prominent role in the shade response of the invasive S. canadensis.

  9. (11) EFFECT OF POWDERED CASTOR BEAN

    African Journals Online (AJOL)

    Adeyinka Odunsi

    The behaviour of the rats was monitored over the three day period. The histology of ... Poisoning by exposure to castor oil seed ... make necklaces in Mexico, because of their ... was 9 to10 weeks. ... responsible for killing 50 percent of the test.

  10. Tough Blends of Polylactide and Castor Oil

    Energy Technology Data Exchange (ETDEWEB)

    Robertson, Megan L.; Paxton, Jessica M.; Hillmyer, Marc A. (UMM)

    2012-10-10

    Poly(l-lactide) (PLLA) is a renewable resource polymer derived from plant sugars with several commercial applications. Broader implementation of the material is limited due to its inherent brittleness. We show that the addition of 5 wt % castor oil to PLLA significantly enhances the overall tensile toughness with minimal reductions in the modulus and no plasticization of the PLLA matrix. In addition, we used poly(ricinoleic acid)-PLLA diblock copolymers, synthesized entirely from renewable resources, as compatibilizers for the PLLA/castor oil blends. Ricinoleic acid, the majority fatty acid comprising castor oil, was polymerized through a lipase-catalyzed condensation reaction. The resulting polymers contained a hydroxyl end-group that was subsequently used to initiate the ring-opening polymerization of L-lactide. The binary PLLA/castor oil blend exhibited a tensile toughness seven times greater than neat PLLA. The addition of block copolymer allowed for control over the morphology of the blends, and even further improvement in the tensile toughness was realized - an order of magnitude larger than that of neat PLLA.

  11. Castor bean response to zinc fertilization

    Energy Technology Data Exchange (ETDEWEB)

    Chaves, Lucia Helena Garofalo; Cunha, Tassio Henrique Cavalcanti da Silva; Lima, Vinicius Mota; Cabral, Paulo Cesar Pinto; Barros Junior, Genival; Lacerda, Rogerio Dantas de [Universidade Federal de Campina Grande (UAEAg/UFCG), PB (Brazil). Unidade Academica de Engenharia Agricola

    2008-07-01

    Zinc is a trace element and it is absolutely essential for the normal healthy growth of plants. This element plays a part of several enzyme systems and other metabolic functions in the plants. Castor beans (Ricinus communis L.) crop is raising attention as an alternative crop for oil and biodiesel production. Despite the mineral fertilization is an important factor for increasing castor beans yield, few researches has been made on this issue, mainly on the use of zinc. In order to evaluate the effects of zinc on growth of this plant an experiment was carried out in a greenhouse, in Campina Grande, Paraiba State, Brazil, from July to December 2007. The substrate for the pot plants was a 6 mm-sieved surface soil (Neossolo Quartzarenico). The experimental design was a completely randomized with three replications. The treatments were composed of five levels of Zn (0; 2; 4; 6 and 8 mg dm{sup -3}), which were applied at the time of planting. One plant of castor bean, cultivar BRS 188 - Paraguacu, was grown per pot after thinning and was irrigated whenever necessary. Data on plant height, number and length of leaves and stem diameter were measured at 21, 34, 77 and 103 days after planting. Under conditions that the experiment was carried out the results showed that the Zn levels used, did not affect the castor bean plants growth. (author)

  12. Phenylpropanoid esters of lesquerella and castor oil

    Science.gov (United States)

    Lesquerella (LO) and castor oil (CO) were esterified at the secondary hydroxyl groups of their 14-hydroxyeicos-cis-11-enoic fatty acids and 12-hydroxyoctadec-cis-9-enoic fatty acids, respectively, with 4-acetoxy-3-methoxycinnamic acid (acetoxyferulic acid). The unconventional esterifications were co...

  13. Tough blends of polylactide and castor oil.

    Science.gov (United States)

    Robertson, Megan L; Paxton, Jessica M; Hillmyer, Marc A

    2011-09-01

    Poly(l-lactide) (PLLA) is a renewable resource polymer derived from plant sugars with several commercial applications. Broader implementation of the material is limited due to its inherent brittleness. We show that the addition of 5 wt % castor oil to PLLA significantly enhances the overall tensile toughness with minimal reductions in the modulus and no plasticization of the PLLA matrix. In addition, we used poly(ricinoleic acid)-PLLA diblock copolymers, synthesized entirely from renewable resources, as compatibilizers for the PLLA/castor oil blends. Ricinoleic acid, the majority fatty acid comprising castor oil, was polymerized through a lipase-catalyzed condensation reaction. The resulting polymers contained a hydroxyl end-group that was subsequently used to initiate the ring-opening polymerization of l-lactide. The binary PLLA/castor oil blend exhibited a tensile toughness seven times greater than neat PLLA. The addition of block copolymer allowed for control over the morphology of the blends, and even further improvement in the tensile toughness was realized-an order of magnitude larger than that of neat PLLA.

  14. (11) EFFECT OF POWDERED CASTOR BEAN

    African Journals Online (AJOL)

    Adeyinka Odunsi

    A study was carried out at the Toxicology laboratory of the Department of Crop and Environmental protection,. Ladoke Akintola University of Technology Ogbomoso, to determine the effects of powdered castor oil seed. (Ricinus communis L.Euphorbiaceae) on kidney, liver, spleen of albino rats. The rats were in five groups, ...

  15. Dissecting Solidago canadensis-soil feedback in its real invasion.

    Science.gov (United States)

    Dong, Li-Jia; Yang, Jian-Xia; Yu, Hong-Wei; He, Wei-Ming

    2017-04-01

    The importance of plant-soil feedback (PSF) has long been recognized, but the current knowledge on PSF patterns and the related mechanisms mainly stems from laboratory experiments. We aimed at addressing PSF effects on community performance and their determinants using an invasive forb Solidago canadensis . To do so, we surveyed 81 pairs of invaded versus uninvaded plots, collected soil samples from these pairwise plots, and performed an experiment with microcosm plant communities. The magnitudes of conditioning soil abiotic properties and soil biotic properties by S. canadensis were similar, but the direction was opposite; altered abiotic and biotic properties influenced the production of subsequent S. canadensis communities and its abundance similarly. These processes shaped neutral S. canadensis -soil feedback effects at the community level. Additionally, the relative dominance of S. canadensis increased with its ability of competitive suppression in the absence and presence of S. canadensis -soil feedbacks, and S. canadensis -induced decreases in native plant species did not alter soil properties directly. These findings provide a basis for understanding PSF effects and the related mechanisms in the field conditions and also highlight the importance of considering PSFs holistically.

  16. Production of Biodiesel from Mixed Waste Cooking and Castor Oil

    Directory of Open Access Journals (Sweden)

    Hadiyanto Hadiyanto

    2018-01-01

    Full Text Available Due to increasing population growth, the consumption and needs of energy increase significantly. This leads Indonesia government to search alternative energy to cover the lacks of fossil energy reserves. Biodiesel is one of the prospective alternative energy which are renewable and environmental friendly. A common problem in large-scale biodiesel production is the sustainability of feedstock and the biodiesel stability. Therefore, the purpose of this study was to evaluate the production of biodiesel from two oil sources i.e. waste cooking oil and castor oil. This study examined the effect of mixed oil ratio on yield, biodiesel characteristics and stability. The physical properties included kinematic viscosity, acid number, saponification number, iodine number and cetane number have been evaluated as function of oil ratio. Yield of biodiesel was obtained at 35.07%, 99.2% and 83.69% for jatropha:castor oil ratio of 1: 0, 1: 2 and 2: 1, respectively. Most of these characteristics showed an increase by increasing the oil ratio. The result concluded that at the ratio of 1:1(v/v was the best characteristic and stability.

  17. Effect of castor oil emulsion eyedrops on tear film composition and stability.

    Science.gov (United States)

    Maïssa, Cécile; Guillon, Michel; Simmons, Peter; Vehige, Joseph

    2010-04-01

    An emulsion eyedrop containing castor oil has been shown to modify the tear film lipid layer and increase tear film stability. The primary objectives of this investigation were to measure the prevalence of castor oil in the tear fluid over time and quantify the effects on the lipid layer. A secondary objective was to quantify the initial effects on ocular symptomatology. The investigation was an open label pilot study on 5 normal and 10 dry eye subjects. A single eyedrop (Castor oil emulsion, Allergan) was instilled in each eye; the tear film appearance and composition were monitored for 4h via in vivo visualisation using the Tearscope and post in vivo tear samples analysis by HPLC. Combined results for both normal and dry eye subjects showed that castor oil was detected up to 4h after a single eyedrop instillation and associated with an increase in the level of tear film lipid. The relative amount of various lipid families was also changed. An increase in tear lipid layer thickness was significant up to one hour post-instillation for the symptomatic sub-population. The changes in tear film characteristics were associated with significantly lower symptoms up to four hours post-instillation for the symptomatic sub-population. This pilot investigation showed that castor oil eyedrops achieved a residence time of at least four hours post-instillation, producing a more stable tear film and an associated significant decrease in ocular symptoms over the entire follow-up period for the symptomatic subjects. 2009 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved.

  18. Four major saponins from Solidago canadensis.

    Science.gov (United States)

    Reznicek, G; Jurenitsch, J; Plasun, M; Korhammer, S; Haslinger, E; Hiller, K; Kubelka, W

    1991-01-01

    Four new bisdesmosidic saponins each containing eight carbohydrate units were isolated from Solidago canadensis. GC, GC-MS, FABMS analysis and mainly the use of 2D NMR techniques allowed their identification as bayogeninglycosides (canadensissaponins 1-4) 3-O- [beta-D-glucopyranosyl-(1----3)-beta-D-glucopyranosyl]-28-O-[alpha-L- rhamnopyranosyl-(1----3)-beta-D-xylopyranosyl-(1----4)-[beta-D- xylopyranosyl-(1----3)]-alpha-L-rhamnopyranosyl-(1----2)-[beta-D- apio-D-furanosyl-(1----3)]-beta-D-6-deoxyglucopyranosyl- (1----]-bayogenin; -(1----2)-[beta-D-apio-D-furanosyl-(1----3)]-ara- binopyranosyl-(1----]-bayogenin; -[alpha-L-rhamnopyranosyl-(1----3)]-beta- D-6-deoxyglucopyranosyl-(1----]-bayogenin and - [alpha-L-rhamnopyranosyl- (1----3)]-arabinopyranosyl-(1----]-bayogenin.

  19. New labdane diterpenes from Solidago canadensis.

    Science.gov (United States)

    Wangensteen, Helle; Phan, Thi T; Rise, Frode; Halvorsen, Trine G; Malterud, Karl E

    2012-01-01

    The ethanol extract of roots of Solidago canadensis yielded eight labdane-type diterpenes. Five of those were new natural compounds (9,13,15,16-bisepoxy-labdane-7-ene-6,15-dione (3a), 13-epi-9,13,15,16-bisepoxy-labdane-7-ene-6,15-dione (3b), 15,16-epoxy-labdane-7,13-diene-6,16-dione (5), 15-ethoxy-9,13,15,16-bisepoxy-labdane-7-ene-6-one (6a) and 13-epi-15-ethoxy-9,13,15,16-bisepoxy-labdane-7-ene-6-one (6b). The known labdane diterpenes deoxysolidagenone (1), solidagenone (2) and 15,16-epoxy-labdane-7,13-diene-6,15-dione (4) were also isolated. Chemical structures were determined using 1D and 2D NMR techniques and MS analysis.

  20. ARTICLE - Inbreeding depression in castor bean (Ricinus communis L. progenies

    Directory of Open Access Journals (Sweden)

    Milton Krieger

    2012-12-01

    Full Text Available The purpose of this study was to investigate inbreeding depression (DE in castor bean. From a population derived from the Guarani cultivar, 60 mother plants were sampled. Three types of progenies were obtained from each one: from self-pollination (AU, from crosses (CR and from open pollination (PL. Grain yield of the progenies was evaluated in two locations. There was a strong interaction of progenies x locations, which led to obtaining estimates within each location. Broad variation was observed in inbreeding depression, with mean values of 6.7% and 13.4%, comparing AU progenies with PL progenies. It was observed that the population has high potential for selecting promising inbred lines. The frequency of mother plants generating progenies with simultaneous high general combination capacity and low inbreeding depression was low. Recurrent selection will increase the occurrence of parent plants associating these two properties, which is necessary for obtaining superior synthetic varieties.

  1. Echinococcus canadensis transmission in the North.

    Science.gov (United States)

    Oksanen, Antti; Lavikainen, Antti

    2015-10-30

    The Echinococcus granulosus complex (EG) is the causative agent of cystic echinococcosis (CE). Northern cervid Echinococcus was previously suggested to be the ancestor of the entire EG. During the last century, it was regarded to have three (or four) different, but often overlapping, transmission cycles in the circumpolar North: the original wolf-wild cervid (reindeer or elk)-cycle; the semi-synanthropic cycle involving sled and hunting dogs and wild cervids; and the synanthropic cycle involving herding dogs and semi-domesticated reindeer. Human infections mainly derived from the latter two cycles. In Fennoscandia, the synanthropic cycle has been eliminated during the last 50 years due to changes in reindeer husbandry methods; machinery making herding dogs largely redundant. Typical to human CE in the North has been the relatively benign nature of the disease compared with CE caused by E. granulosus sensu stricto. The metacestodes in humans and in the natural cervid hosts predominantly appear in the lungs. The causative agents have been identified as EG mitochondrial genotypes G8 and G10, now together with G6 (camel), G7 (pig) and G9 genotypes constituting the Echinococcus canadensis species. Based on recent findings in reindeer in Yakutia, G6 might also be recognised among cervid genotypes. The geographical distribution of both G8 and G10 is circumpolar, with G10 currently apparently more prevalent both in the Palearctic and Nearctic. Because of the disappearance of the working dog, E. canadensis in Fennoscandia is again highly dependent on the wolf, as it was before domestication of the dog. Pet and sled dogs, if their number further increases, may to a minor part participate in the life cycle. Human CE in the North was mostly diagnosed by mass chest tuberculosis radiography campaigns, which have been discontinued. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Constituents of the essential oil in Solidago canadensis L. from Eurasia

    Directory of Open Access Journals (Sweden)

    Olga Shelepova

    2018-02-01

    Full Text Available Hydro distilled essential oils in air-dry samples of aerial parts of Solidago canadensis L., (Asteraceae from eight local invasive populations were investigated by GC-MS analysis. A comparative study on quantity and composition of the essential oils obtained from plants, growing in different ecological and climatic conditions, ontogenesis phase and different plant organs was carried out The major compounds detected in oil samples of S. canadensis were α-pinene (1.3 - 61.27%, limonene (0.5 - 22.5%, bornyl acetat (3.4 - 29.8% and germacrene D (1.8 - 39.2%. Samples from inflorescences contained the maximal percentage of monoterpene hydrocarbons, while the leaves' samples showed the maximal cumulative percentage of sesquiterpene and monoterpene hydrocarbons. Data obtained from our studies confirm the availability of alien invasive species Solidago canadensis for medicine and many other purposes. The variability of the qualitative and quantitative composition of essential oils in different geographical locations will allow futher selection of form containing the maximum amount of active substances.

  3. First confirmed record of Elodea canadensis Michx. (Hydrocharitaceae in Greece

    Directory of Open Access Journals (Sweden)

    Poulis Georgios

    2017-12-01

    Full Text Available The paper confirms the presence of Elodea canadensis Michx. in Greece and outlines the history of contradictory relevant reports. This is also the first report of the species′ presence in the transboundary lake Great Prespa.

  4. Antimicrobial fungal endophytes from the botanical medicine goldenseal (Hydrastis canadensis).

    Science.gov (United States)

    Egan, Joseph M; Kaur, Amninder; Raja, Huzefa A; Kellogg, Joshua J; Oberlies, Nicholas H; Cech, Nadja B

    2016-09-01

    The potential of fungal endophytes to alter or contribute to plant chemistry and biology has been the topic of a great deal of recent interest. For plants that are used medicinally, it has been proposed that endophytes might play an important role in biological activity. With this study, we sought to identify antimicrobial fungal endophytes from the medicinal plant goldenseal ( Hydrastis canadensis L., Ranunculaceae), a plant used in traditional medicine to treat infection. A total of 23 fungal cultures were obtained from surface-sterilized samples of H. canadensis roots, leaves and seeds. Eleven secondary metabolites were isolated from these fungal endophytes, five of which had reported antimicrobial activity. Hydrastis canadensis plant material was then analyzed for the presence of fungal metabolites using liquid chromatography coupled to high resolving power mass spectrometry. The antimicrobial compound alternariol monomethyl ether was detected both as a metabolite of the fungal endophyte Alternaria spp. isolated from H. canadensis seeds, and as a component of an extract from the H. canadensis seed material. Notably, fungi of the Alternaria genus were isolated from three separate accessions of H. canadensis plant material collected in a time period spanning 5 years. The concentration of alternariol monomethyl ether (991 mg/kg in dry seed material) was in a similar range to that previously reported for metabolites of ecologically important fungal endophytes. The seed extracts themselves, however, did not possess antimicrobial activity.

  5. Optimization of biodiesel production from castor oil.

    Science.gov (United States)

    da Silva, Nivea de Lima; Maciel, Maria Regina Wolf; Batistella, César Benedito; Maciel Filho, Rubens

    2006-01-01

    The transesterification of castor oil with ethanol in the presence of sodium ethoxide as catalyst is an exceptional option for the Brazilian biodiesel production, because the castor nut is quite available in the country. Chemically, its oil contains about 90% of ricinoleic acid that gives to the oil some beneficial characteristics such as its alcohol solubility at 30 degrees C. The transesterification variables studied in this work were reaction temperature, catalyst concentration and alcohol oil molar ratio. Through a star configuration experimental design with central points, this study shows that it is possible to achieve the same conversion of esters carrying out the transesterification reaction with a smaller alcohol quantity, and a new methodology was developed to obtain high purity biodiesel.

  6. Isolation and characterization of polymorphic microsatellite loci from the invasive plant Solidago canadensis (Asteraceae).

    Science.gov (United States)

    Zhao, S-Y; Sun, S-G; Guo, Y-H; Chen, J-M; Wang, Q-F

    2012-02-17

    Solidago canadensis, a clonal herb originally from North America (common name: Canada goldenrod), is an invasive species in many countries. We developed microsatellite primers for this species. Eleven polymorphic loci were generated and primers were designed. Polymorphism of these 11 loci was assessed in 35 plants from two populations (Wuhan and Shanghai) in China. The number of alleles per locus ranged from 3 to 14. The observed and expected heterozygosities varied from 0.0732 to 0.7391 and from 0.1177 to 0.8687, respectively. These microsatellite markers will be useful tools for studies of population genetics in the native and invasive range of this species.

  7. Synthesis of branched naphthoquinones from castor oil

    Directory of Open Access Journals (Sweden)

    Adriano Olímpio da Silva

    2012-06-01

    Full Text Available The naphthoquinones are cyclic aromatic α,β-dienonas with a basic framework derived from naphthalene. They are also found in many higher plants, algae, fungi and as the product of the  metabolism  of some  bacteria  having large biologica activity described in the literature such as antibacterial, anti-inflammatory, antitumor, anticancer and trypanocidal [1-3]. Castor oil is an abundant raw material in Brazil of great versatility and, it is present in biodiesel production, surfactants, cosmetics and others. Considering the importance of naphthoquinones and, the availability of the ricinoleic acid from castor oil, the aim of this study was the preparation of new branched naphthoquinones in order to test their trypanocidal activity. Castor oil was submitted to saponification with sodium hydroxide, ethanol and water under reflux for 6 h. We then carried out an acid hydrolysis with hydrochloric acid and the formed ricinoleic acid was extracted with ethyl acetate. Following, through Kochi-Anderson addition reaction it was performed the alkylation of a naphthoquinone 1 and 2, using ammonium persulfate, silver nitrate, acetonitrile and water, under heating at 70-80 ° C during 3 h, to give the branched naphthoquinones 4 and 5 (scheme 1. The naphthoquinone 3 will be similarly submitted to this procedure. The naphthoquinones 4 and 5 were purified by column chromatography on sílica gel using hexane as the eluent. The compounds were characterized by mass spectrometry and 1H and 13CNMR spectroscopy.

  8. LHCb: CASTORFS - A Filesystem to Access CASTOR

    CERN Multimedia

    Mazurov, A

    2009-01-01

    CASTOR provides a powerful and rich interface for managing files and pools of files backed by tape-storage. The API is modelled very closely on that of a POSIX filesystem, where part of the actual I/O part is handled by the rfio library. While the API is very close to POSIX it is still separated, which unfortunately makes it impossible to use standard tools and scripts straight away. This is particularly inconvenient when applications are written in languages other than C/C++ such as is frequently the case in web-apps. Here up to now the only the recourse was to use command-line utilities and parse their output, which is clearly a kludge. We have implemented a complete POSIX filesystem to access CASTOR using FUSE (Filesystem in Userspace) and have successfully tested and used this on SLC4 and SLC5 (both in 32 and 64 bit). We call it CastorFS. In this paper we will present its architecture and implementation, with emphasis on performance and caching aspects.

  9. CASTORFS - A filesystem to access CASTOR

    Energy Technology Data Exchange (ETDEWEB)

    Mazurov, Alexander; Neufeld, Niko, E-mail: alexander.mazurov@cern.c [CERN, Geneve 23 (Switzerland)

    2010-04-01

    CASTOR provides a powerful and rich interface for managing files and pools of files backed by tape-storage. The API is modelled very closely on that of a POSIX filesystem, where part of the actual I/O part is handled by the rfio library. While the API is very close to POSIX it is still separated, which unfortunately makes it impossible to use standard tools and scripts straight away. This is particularly inconvenient when applications are written in languages other than C/C++ such as is frequently the case in web-apps. Here up to now the only the recourse was to use command-line utilities and parse their output, which is clearly a kludge. We have implemented a complete POSIX filesystem to access CASTOR using FUSE (Filesystem in Userspace) and have successfully tested and used this on SLC4 and SLC5 (both in 32 and 64 bit). We call it CastorFS. In this paper we will present its architecture and implementation, with emphasis on performance and caching aspects.

  10. Single nucleotide polymorphisms for assessing genetic diversity in castor bean (Ricinus communis

    Directory of Open Access Journals (Sweden)

    Rabinowicz Pablo D

    2010-01-01

    Full Text Available Abstract Background Castor bean (Ricinus communis is an agricultural crop and garden ornamental that is widely cultivated and has been introduced worldwide. Understanding population structure and the distribution of castor bean cultivars has been challenging because of limited genetic variability. We analyzed the population genetics of R. communis in a worldwide collection of plants from germplasm and from naturalized populations in Florida, U.S. To assess genetic diversity we conducted survey sequencing of the genomes of seven diverse cultivars and compared the data to a reference genome assembly of a widespread cultivar (Hale. We determined the population genetic structure of 676 samples using single nucleotide polymorphisms (SNPs at 48 loci. Results Bayesian clustering indicated five main groups worldwide and a repeated pattern of mixed genotypes in most countries. High levels of population differentiation occurred between most populations but this structure was not geographically based. Most molecular variance occurred within populations (74% followed by 22% among populations, and 4% among continents. Samples from naturalized populations in Florida indicated significant population structuring consistent with local demes. There was significant population differentiation for 56 of 78 comparisons in Florida (pairwise population ϕPT values, p Conclusion Low levels of genetic diversity and mixing of genotypes have led to minimal geographic structuring of castor bean populations worldwide. Relatively few lineages occur and these are widely distributed. Our approach of determining population genetic structure using SNPs from genome-wide comparisons constitutes a framework for high-throughput analyses of genetic diversity in plants, particularly in species with limited genetic diversity.

  11. Interpenetrating polymer networks based on polyol modified castor ...

    Indian Academy of Sciences (India)

    Unknown

    glycerol modification of castor oil results in more crosslinked and stiffer IPNs possessing better mechanical properties over that of unmodified castor oil polyurethane/. PHEMA IPNs. 3.5 Chemical resistance. The percentage weight loss of IPNs were determined in. H2SO4, CH3COOH, HCl, HNO3, NaCl and NaOH etc and.

  12. Synthesis and characterization of castor oil based polyurethane ...

    Indian Academy of Sciences (India)

    Unknown

    Abstract. A series of interpenetrating polymer networks (IPNs) of castor oil based polyurethane/polyacrylo- nitrile (PU/PAN: 80/20, 60/40, 50/50, 40/60 and 20/80) were synthesized by condensation reaction of castor oil with methylene diisocyanate and acrylonitrile, employing benzoyl peroxide (BPO) and ethylene glycol ...

  13. Allelopathic effect of aqueous extract of fresh leaf castor beans ...

    African Journals Online (AJOL)

    Allelopathy defines the production of specific biomolecules (allelochemical) by a plant that can induce positive or negative impacts on another culture. The crop of castor beans (Ricinus communis L.) is being economically valued and receiving attention, mainly by the biodiesel production, castor oil and animal feeding.

  14. Novel alpha-hydroxy phosphonic acids via castor oil

    Science.gov (United States)

    Hydroxy fatty acids (HFAs) have found a number of uses in today’s market, with uses ranging from materials to pharmaceuticals. Castor oil has served as a versatile HFA; its principle component, ricinoleic acid, can be isolated from castor oil and has been modified extensively for a number of applica...

  15. Synthesis and characterization of castor oil-based polyurethane

    Indian Academy of Sciences (India)

    Polyurethane (PU) based on polyol, derived from castor oil has been synthesized and characterized for potential use as a base material for electrolytes. Transesterification process of castor oil formed a polyol with hydroxyl value of 190 mg KOH g–1 and molecular weight of 2786 g mol–1. The polyols together with 4 ...

  16. Screening of spontaneous castor bean accesses for genetic ...

    African Journals Online (AJOL)

    ... discriminant power between the castor bean accesses, being the multivariate analysis efficient in this process. The castor bean accesses ACS-001 CRSP and ACS-001-MASP are promising for introduction in genetic improvement programs of this culture. Keywords: Ricinus communis L., genotype, multivariate statistics, ...

  17. Phytoextraction of metals and rhizoremediation of PAHs in co-contaminated soil by co-planting of Sedum alfredii with ryegrass (Lolium perenne) or castor (Ricinus communis).

    Science.gov (United States)

    Wang, Kai; Huang, Huagang; Zhu, Zhiqiang; Li, Tingqiang; He, Zhenli; Yang, Xiaoe; Alva, Ashok

    2013-01-01

    A pot experiment was conducted to investigate the potential for phytoextraction of heavy metals and rhizoremediation of polycyclic aromatic hydrocarbons (PAHs) in co-contaminated soil by co-planting a cadmium/zinc (Cd/Zn) hyperaccumulator and lead (Pb) accumulator Sedum alfredii with ryegrass (Lolium perenne) or castor (Ricinus communis). Co-planting with castor decreased the shoot biomass of S. alfredii as compared to that in monoculture. Cadmium concentration in S. alfredii shoot significantly decreased when grown with ryegrass or castor as compared to that in monoculture. However, no reduction of Zn or Pb concentration in S. alfredii shoot was detected in co-planting treatments. Total removal of either Cd, Zn, or Pb by plants was similar across S. alfredii monoculture or co-planting with ryegrass or castor, except enhanced Pb removal in S. alfredii and ryegrass co-planting treatment. Co-planting of S. alfredii with ryegrass or castor significantly enhanced the pyrene and anthracene dissipation as compared to that in the bare soil or S. alfredii monoculture. This appears to be due to the increased soil microbial population and activities in both co-planting treatments. Co-planting of S. alfredii with ryegrass or castor provides a promising strategy to mitigate both metal and PAH contaminants from co-contaminated soils.

  18. Diversity of Castor (Ricinus communis L.) in Ethiopia አህፅርኦት ...

    African Journals Online (AJOL)

    In Ethiopia, Castor grows as annual in the low lands to small tree perennial in the high lands. India, china, Brazil and USA are the major castor producers globally. Castor bears seeds that containing highly valuable industrial oil. Castor oil is unique among vegetable oil because it is the only commercial source of a ...

  19. Castor halls. A problem for the century; Castor-Hallen. Ein Jahrhundertproblem

    Energy Technology Data Exchange (ETDEWEB)

    Stay, Jochen

    2017-01-15

    The interim storage of high-level radioactive waste will last longer than asserted. The existing halls for the castor casks are not appropriate for this task with respect to safety. The casks themselves are not designed for long-term storage. Politics obviously do not consider the problems seriously.

  20. CASTOR detector. Model, objectives and simulated performance

    International Nuclear Information System (INIS)

    Angelis, A. L. S.; Mavromanolakis, G.; Panagiotou, A. D.; Aslanoglou, X.; Nicolis, N.; Lobanov, M.; Erine, S.; Kharlov, Y. V.; Bogolyubsky, M. Y.; Kurepin, A. B.; Chileev, K.; Wlodarczyk, Z.

    2001-01-01

    It is presented a phenomenological model describing the formation and evolution of a Centauro fireball in the baryon-rich region in nucleus-nucleus interactions in the upper atmosphere and at the LHC. The small particle multiplicity and imbalance of electromagnetic and hadronic content characterizing a Centauro event and also the strongly penetrating particles (assumed to be strangelets) frequently accompanying them can be naturally explained. It is described the CASTOR calorimeter, a sub detector of the ALICE experiment dedicated to the search for Centauro in the very forward, baryon-rich region of central Pb+Pb collisions at the LHC. The basic characteristics and simulated performance of the calorimeter are presented

  1. Production of Detergent from Castor Oil

    OpenAIRE

    Abubakar Garba ISAH

    2006-01-01

    This research work was carried out with the objective of extraction of oil from castor seeds and its utilization to produce a synthetic detergent. Solvent extraction method was employed in extracting the oil and the total percent oil yield was found to be 23.8%. The experimentally determined saponification value of the oil was 183.7275mgKOH/g of oil. The detergent efficiency, determined as a measure of the foamability of the detergent was found to be 2.6cm. The pH tests revealed mildly basic ...

  2. Solidago canadensis und Solidago gigantea in Frankfurt am Main

    OpenAIRE

    Ottich, Indra

    2015-01-01

    Die beiden nordamerikanischen Goldruten-Arten Solidago canadensis und S. gigantea sind in Mitteleuropa weit verbreitet. Häufig wird nicht zwischen den beiden Arten unterschieden, die Angaben zur Ökologie scheinen für beide Arten weitgehend identisch. Durch eine Rasterkartierung des Stadtgebietes von Frankfurt am Main wird gezeigt, dass die Arten jedoch unterschiedliche Ansprüche haben. Während Solidago canadensis sehr häufig ist und alle typisch städtischen Bereiche besiedelt, wird Solidago g...

  3. Copper toxicity in leaves of Elodea canadensis Michx.

    Science.gov (United States)

    Malec, Przemysław; Maleva, Maria; Prasad, M N V; Strzałka, Kazimierz

    2009-05-01

    Elodea canadensis (Canadian waterweed) has an ability to accumulate and bioconcentrate heavy metals. In this work, selected cellular responses for Cu treatment were studied in leaves of E. canadensis. Short term experiments, i.e. 1 week exposure to 0.5, 1, 5, and 10 microM of Cu indicated that concentrations up to 10 microM Cu causes a pronounced accumulation of photosynthetic pigments, a drastic degradation of soluble proteins with molecular weight above 18 kDa and a rapid accumulation of polypeptides with molecular weight below 14 kDa. The connection of these observations with copper detoxification mechanisms in aquatic macrophytes are discussed.

  4. Castor Oil Based Polyurethanes: Synthesis and Characterization

    Science.gov (United States)

    Macalino, AD; Salen, VA; Reyes, LQ

    2017-09-01

    In this study, polyurethanes based on castor oil and 1,6-hexamethylene diisocyanate (HMDI) were synthesized with varying weight ratio of the castor oil and HMDI. The formation of urethane linkages was verified through the use of a fourier transform infrared spectroscopy (FTIR). The hydrophilicity of the films was evaluated through the use of a contact angle meter and it was found that the contact angle of all the films were below 90 degrees which confirms their hydrophilicity. The thermal stability of the PU films were studies through the use of a thermal gravimetric analyzer and found that all of the polyurethane films exhibited two weight loss events at elevated temperatures wherein the first weight loss event was observed to occur at 285°C to 384°C while the second weight loss event was observed at around 521°C to 551°C. The hardness, elastic modulus, and tensile elongation of the PU films were determined by using a universal testing machine (UTM) where it was found out that the hardness and the elastic modulus of the film is directly proportional with HMDI loading while the tensile elongation is inversely proportional to it. Lastly, it was known through the swelling studies of the PU films that it does not swell, this is due to the presence of unreacted triglycerides in the material, which prevents water from permeating to the films.

  5. Mixed Field Modification of Thermally Cured Castor Oil Based Polyurethanes

    International Nuclear Information System (INIS)

    Mortley, A.

    2006-01-01

    Thermally cured polyurethanes were prepared from castor oil and hexamethylene diisocyanatee (HMDI). Due to the long aliphatic chain of the castor oil component of polyurethane, thermal curing of castor oil based polyurethane (COPU) is limited by increasing polymer viscosity. To enhance further crosslinking, COPUs were subjected to a range of accumulated doses (0.0-3.0 MGy) produced by the mixed ionizing field of the SLOWPOKE-2 research reactor. The physico-mechanical properties of COPU, unirradiated and irradiated, were characterized by mechanical tests. Increased bond formation resulting from radiation-induced crosslinking was confirmed by favorable increases in mechanical properties and by solid-state 13 C -NMR and FTIR spectra

  6. Biodegradation of polyurethane derived from castor oil

    Directory of Open Access Journals (Sweden)

    José M. Cangemi

    2008-09-01

    Full Text Available The aim of this research was to study the biodegradation of a polymer derived from castor oil, which is a renewable, natural material that is a practical alternative for the replacement of traditional polyurethane foams. Due to its molecular structure, which contains polyester segments derived from vegetable oil, the polymeric surface is susceptible to microorganism attack. This study tested the biological degrading agent that was in contact with the microorganisms resulting from microbiological grease degrading agents, when foam was inoculated. Solid-media agar-plate tests were conducted for their potential to evaluate the biodegradation of polymeric particles by specific strains of microorganisms during 216 hours. The growth rate was defined. This technique provides a way of distinguishing the degradation abilities of microorganisms from the degradability of materials.

  7. Production of Detergent from Castor Oil

    Directory of Open Access Journals (Sweden)

    Abubakar Garba ISAH

    2006-07-01

    Full Text Available This research work was carried out with the objective of extraction of oil from castor seeds and its utilization to produce a synthetic detergent. Solvent extraction method was employed in extracting the oil and the total percent oil yield was found to be 23.8%. The experimentally determined saponification value of the oil was 183.7275mgKOH/g of oil. The detergent efficiency, determined as a measure of the foamability of the detergent was found to be 2.6cm. The pH tests revealed mildly basic properties. The color, scent and efficiency of the detergent were improved with the addition of bleaching agent, perfume and foaming agents respectively.

  8. Castor Oil Transesterification Catalysed by Liquid Enzymes

    DEFF Research Database (Denmark)

    Andrade, Thalles; Errico, Massimiliano; Christensen, Knud Villy

    2017-01-01

    In the present work, biodiesel production by reaction of non-edible castor oil with methanol under enzymatic catalysis is investigated. Two liquid enzymes were tested: Eversa Transform and Resinase HT. Reactions were performed at 35 °C and with a molar ratio of methanol to oil of 6:1. The reaction...... time was 8 hours. Stepwise addition of methanol was necessary to avoid enzyme inhibition by methanol. In order to minimize the enzyme costs, the influence of enzyme activity loss during reuse of both enzymes was evaluated under two distinct conditions. In the former, the enzymes were recovered...... and fully reused; in the latter, a mixture of 50 % reused and 50 % fresh enzymes was tested. In the case of total reuse after three cycles, both enzymes achieved only low conversions. The biodiesel content in the oil-phase using Eversa Transform was 94.21 % for the first cycle, 68.39 % in the second, and 33...

  9. Castor oil for induction of labour: a retrospective study.

    Science.gov (United States)

    Neri, Isabella; Dante, Giulia; Pignatti, Lucrezia; Salvioli, Chiara; Facchinetti, Fabio

    2018-08-01

    The aim of this study is to investigate the safety and efficacy of castor oil to induce labour. A retrospective observational case control study was conducted over five years. Castor oil was proposed to women referred to the Birth Centre (Castor Oil group (COG)). They were compared to women who chose to be followed by the traditional doctor-led unit (control group (CG)). Castor oil was administered in a 60 ml single dose in 200 ml of warm water. Inclusion criteria were gestational age between 40 and 41 weeks plus premature rupture of membranes between 12 and 18 hours or amniotic fluid index ≤4 or Bishop Score of ≤4 or absence of spontaneous labour over 41 + 4 weeks. Pharmacological induction of labour was required for 18 women in the COG (45%) and 36 in the CG (90%) (p oil showed a higher incidence of vaginal delivery, whereas the incidence of caesarean section was lower in the COG, but no statistical significance was reached. The use of castor oil is related to a higher probability of labour initiation within 24 hours. Castor oil can be considered a safe non-pharmacological method for labour induction.

  10. Discovery and Characterization of Novel Signatures from the Ricinus communis L. (Castor Bean) Genome

    National Research Council Canada - National Science Library

    O'Connell, Kevin P; Skowronski, Evan W; Dretchen, Kenneth L; Leshin, Jonathan A; Weeks, Andrea

    2006-01-01

    Given the infamous toxicity of ricin and the industrial usefulness of castor oil, there is a surprising lack of information about the genetic diversity of the species that produces both, the castor bean plant (Ricinus communis L...

  11. Castor Oil: Properties, Uses, and Optimization of Processing Parameters in Commercial Production.

    Science.gov (United States)

    Patel, Vinay R; Dumancas, Gerard G; Kasi Viswanath, Lakshmi C; Maples, Randall; Subong, Bryan John J

    2016-01-01

    Castor oil, produced from castor beans, has long been considered to be of important commercial value primarily for the manufacturing of soaps, lubricants, and coatings, among others. Global castor oil production is concentrated primarily in a small geographic region of Gujarat in Western India. This region is favorable due to its labor-intensive cultivation method and subtropical climate conditions. Entrepreneurs and castor processors in the United States and South America also cultivate castor beans but are faced with the challenge of achieving high castor oil production efficiency, as well as obtaining the desired oil quality. In this manuscript, we provide a detailed analysis of novel processing methods involved in castor oil production. We discuss novel processing methods by explaining specific processing parameters involved in castor oil production.

  12. Castor oil for induction of labour: not harmful, not helpful.

    Science.gov (United States)

    Boel, Machteld Elisabeth; Lee, Sue Jean; Rijken, Marcus Johannes; Paw, Moo Koo; Pimanpanarak, Mupawjay; Tan, Saw Oo; Singhasivanon, Pratap; Nosten, François; McGready, Rose

    2009-10-01

    Castor oil is one of the most popular drugs for induction of labour in a non-medical setting; however, published data on safety and effectiveness of this compound to induce labour remain sparse. To assess the safety and effectiveness of castor oil for induction of labour in pregnancies with an ultrasound estimated gestational at birth of more than 40 weeks. Data were extracted from hospital-based records of all pregnant women who attended antenatal clinics on the Thai-Burmese border and who were more than 40 weeks pregnant. The effectiveness of castor oil to induce labour was expressed as time to birth and analysed with a Cox proportional hazards regression model. Measures associated with safety were fetal distress, meconium-stained amniotic fluid, tachysystole of the uterus, uterine rupture, abnormal maternal blood pressure during labour, Apgar scores, neonatal resuscitation, stillbirth, post-partum haemorrhage, severe diarrhoea and maternal death. Proportions were compared using Fisher's exact test. Of 612 women with a gestation of more than 40 weeks, 205 received castor oil for induction and 407 did not. The time to birth was not significantly different between the two groups (hazard ratio 0.99 (95% confidence interval: 0.81 to 1.20; n = 509)). Castor oil use was not associated with any harmful effects on the mother or fetus. Castor oil for induction of labour had no effect on time to birth nor were there any harmful effects observed in this large series. Our findings leave no justification for recommending castor oil for this purpose.

  13. A new labdane diterpene from the flowers of Solidago canadensis.

    Science.gov (United States)

    Bradette-Hébert, Marie-Eve; Legault, Jean; Lavoie, Serge; Pichette, André

    2008-01-01

    A new labdane diterpene, 9alpha,16xi-dihydroxy-6-oxo-7,13-labdadien-15,16-olide (solicanolide, 1) and six known compounds identified as quercetin (2), 3-O-caffeoylquinic acid (3, neochlorogenic acid), 5-O-caffeoylquinic acid (4, chlorogenic acid), 4,5-di-O-caffeoylquinic acid (5), 3,5-di-O-caffeoylquinic acid (6) and 3,4-di-O-caffeoylquinic acid (7) were isolated from the flowers of Solidago canadensis. To our knowledge, compound 7 was isolated for the first time in S. canadensis. This work describes the isolation of compounds 1-7 and the structure elucidation of a new compound identified as compound 1. Solicanolide (1) showed cytotoxic activity against A549 (IC(50): 13+/-2 microM), DLD-1 (IC(50): 26+/-2 microM) and WS1 (IC(50): 17+/-1 microM) cell lines.

  14. Effect of lead toxicity on aquatic macrophyte Elodea canadensis Michx.

    Science.gov (United States)

    Dogan, Muhittin; Saygideger, Saadet Demirors; Colak, Ugur

    2009-08-01

    Effects of Pb accumulation on the contents of chlorophylls (a and b), carotenoid, ascorbic acid (AsA), non-protein SH groups and protein were investigated in aquatic macrophyte Elodea canadensis. Pb accumulation in E. canadensis tissues increased with increasing metal concentrations. The increases at 1, 10 and 100 mg/L Pb are about 12.0, 44.6 and 71.1 times greater than control, respectively. Contents of chlorophylls, carotenoid and protein were adversely affected by Pb accumulation. Induction of non-protein SH groups and AsA showed that Pb accumulation caused oxidative stress. It is also possible that increased non-protein SH groups by Pb accumulation may be due to their role in Pb detoxification.

  15. Batch study of uranium biosorption by Elodea canadensis biomass

    International Nuclear Information System (INIS)

    Zheng-ji Yi; University of Science and Technology Beijing, Haidian District, Beijing; Jun Yao; Chinese University of Geosciences, Beijing; Mi-jia Zhu; Hui-lun Chen; Fei Wang; Zhi-min Yuan; Xing Liu

    2016-01-01

    The adsorption of U(VI) onto Elodea canadensis was studied via a batch equilibrium method. Kinetic investigation indicated that the U(VI) adsorption by E. canadensis reached an equilibrium in 120 min and followed pseudo-second-order kinetics. The solution pH was the most important parameter controlling adsorption of U(VI) and the optimum pH for U(VI) removal is 6.0. The U(VI) biosorption can be well described by Langmuir model. IR spectrum analysis revealed that -NH 2 , -OH, C=O and C-O could bind strongly with U(VI). XPS spectrum analysis implied that ion exchange and coordination mechanism could be involved in the U(VI) biosorption process. (author)

  16. Isolation and antifungal screening of endophytic fungi from Erigeron canadensis

    Directory of Open Access Journals (Sweden)

    Xuelian Bai

    2017-07-01

    Full Text Available Sixteen fungal strains isolated from the Erigeron canadensis, one of traditional Chinese medicines used to treat the pathogenic infection and dysentery, were evaluated for their antifungal activities against one human pathogen Candida albicans, and two phytopathogens, Colletotrichum fructicola and Rhizoctonia cerealis. The bioassay results indicated that the ethyl acetate extract of the fermentation broth of these fungal endophytes had stronger antimicrobial activities. Among these endophytic strains, the ethyl acetate extracts of strains NPR003 and NPR005 showed the strongest inhibitory effects and has potential application in the discovery of new antifungal agents. This was the first report on the isolation of endophytic fungi from E. canadensis and evaluation of their antifungal activities.

  17. The Eurasian beaver (Castor fiber) is apparently not a host to blood parasites in Norway.

    Science.gov (United States)

    Cross, Hannah B; Campbell-Palmer, Róisín; Girling, Simon; Rosell, Frank

    2012-11-23

    Parasites can alter the physiology and behaviour of host species and negatively impact on their fitness thus affecting population densities. This is the first investigation into the presence of blood parasites in the Eurasian beaver (Castor fiber); a species that has been the subject of many translocation and reintroduction programmes. Two hundred and seventy blood slides prepared from the blood of 27 beavers from southern Norway were microscopically analysed for the presence of blood parasites. This study reports an absence of blood parasites in the Norwegian Eurasian beavers sampled. Copyright © 2012 Elsevier B.V. All rights reserved.

  18. Castor Oil: Properties, Uses, and Optimization of Processing Parameters in Commercial Production

    OpenAIRE

    Patel, Vinay R.; Dumancas, Gerard G.; Kasi Viswanath, Lakshmi C.; Maples, Randall; Subong, Bryan John J.

    2016-01-01

    Castor oil, produced from castor beans, has long been considered to be of important commercial value primarily for the manufacturing of soaps, lubricants, and coatings, among others. Global castor oil production is concentrated primarily in a small geographic region of Gujarat in Western India. This region is favorable due to its labor-intensive cultivation method and subtropical climate conditions. Entrepreneurs and castor processors in the United States and South America also cultivate cast...

  19. Castor oil induces laxation and uterus contraction via ricinoleic acid activating prostaglandin EP3 receptors

    OpenAIRE

    Tunaru, Sorin; Althoff, Till F.; Nüsing, Rolf M.; Diener, Martin; Offermanns, Stefan

    2012-01-01

    Castor oil is one of the oldest drugs. When given orally, it has a laxative effect and induces labor in pregnant females. The effects of castor oil are mediated by ricinoleic acid, a hydroxylated fatty acid released from castor oil by intestinal lipases. Despite the wide-spread use of castor oil in conventional and folk medicine, the molecular mechanism by which ricinoleic acid acts remains unknown. Here we show that the EP3 prostanoid receptor is specifically activated by ricinoleic acid and...

  20. Cellular proton dynamics in Elodea canadensis leaves induced by cadmium.

    Science.gov (United States)

    Tariq Javed, M; Lindberg, Sylvia; Greger, Maria

    2014-04-01

    Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca(2+)]cyt was also investigated as the [Ca(2+)]cyt and [pH]cyt homeostasis are closely linked. The cellular H(+) and Ca(2+) concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca(2+) concentration. Time course studies revealed that changes in [Ca(2+)]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  1. [Allelopathic effects of invasive weed Solidago canadensis on native plants].

    Science.gov (United States)

    Mei, Lingxiao; Chen, Xin; Tang, Jianjun

    2005-12-01

    With growth chamber method, this paper studied the allelopathic potential of invasive weed Solidago canadensis on native plant species. Different concentration S. canadensis root and rhizome extracts were examined, and the test plants were Trifolium repens, Trifolium pretense, Medicago lupulina, Lolium perenne, Suaeda glauca, Plantago virginica, Kummerowia stipulacea, Festuca arundinacea, Ageratum conyzoides, Portulaca oleracea, and Amaranthus spinosus. The results showed that the allelopathic inhibitory effect of the extracts from both S. canadensis root and rhizome was enhanced with increasing concentration, and rhizome extracts had a higher effect than root extracts. At the lowest concentration (1:60), root extract had little effect on the seed germination and seedling growth of T. repens, but rhizome extract could inhibit the germination of all test plants though the inhibitory effect varied with different species. The inhibition was the greatest for grass, followed by forb and legume. 1:60 (m:m) rhizome extract had similar effects on seed germination and radicel growth, but for outgrowth, the extract could inhibit Kummerowia stipulacea, Amaranthus spinosus and Festuca arundinacea, had no significant impact on Lolium perenne, Plantago virginica, Ageratum conyzoides, Portulaca oleracea and Amaranthus spinosus, and stimulated Trifolium repens, Trifolium pretense and Medicago lupulina.

  2. Echinococcus multilocularis and Echinococcus canadensis in wolves from western Canada.

    Science.gov (United States)

    Schurer, Janna M; Gesy, Karen M; Elkin, Brett T; Jenkins, Emily J

    2014-02-01

    Echinococcus species are important parasites of wildlife, domestic animals and people worldwide; however, little is known about the prevalence, intensity and genetic diversity of Echinococcus tapeworms in Canadian wildlife. Echinococcus tapeworms were harvested from the intestines of 42% of 93 wolves (Canis lupus) from five sampling regions in the Northwest Territories, Manitoba and Saskatchewan, and visually identified to genus level by microscopic examination. Genetic characterization was successful for tapeworms from 30 wolves, and identified both Echinococcus canadensis and Echinococcus multilocularis in all sampling locations. Mixed infections of E. canadensis/E. multilocularis, as well as the G8/G10 genotypes of E. canadensis were observed. These findings suggest that wolves may be an important definitive host for both parasite species in western Canada. This represents the first report of wolves naturally infected with E. multilocularis in North America, and of wolves harbouring mixed infections with multiple species and genotypes of Echinococcus. These observations provide important information regarding the distribution and diversity of zoonotic species of Echinococcus in western North America, and may be of interest from public health and wildlife conservation perspectives.

  3. Castor oil biodiesel as an alternative fuel for diesel engines

    International Nuclear Information System (INIS)

    Benavides, Alirio; Benjumea, Pedro; Pashova, Veselina

    2007-01-01

    In this paper, a study related to the production and use of castor oil biodiesel is presented. The maximum methyl esters yield of the castor oil transesterification reaction is obtained under the following conditions: ambient temperature, a molar ratio of methanol to vegetable oil equal to 9 and a catalyst percentage equal to 0.8%. The castor oil biodiesel can be blended with petroleum diesel as far as 15% in such way that the resulting blend complies with national and international technical standards for diesel fuels. Its high viscosity becomes the main difficulty for using castor oil biodiesel in engines. However this biofuel exhibits excellent cold flow properties (low values of cloud and pour points). The motor tests using castor oil biodiesel petroleum diesel blends, for the biodiesel proportion tested; show that a biodiesel percentage increase leads to an increase in the specific fuel consumption, a decrease in the fuel air ratio, a slight decrease in smoke opacity, while the fuel conversion efficiency and the CO and CO 2 emissions practically remain constants

  4. A Review of Hypothesized Determinants Associated with Bighorn Sheep (Ovis canadensis Die-Offs

    Directory of Open Access Journals (Sweden)

    David S. Miller

    2012-01-01

    Full Text Available Multiple determinants have been hypothesized to cause or favor disease outbreaks among free-ranging bighorn sheep (Ovis canadensis populations. This paper considered direct and indirect causes of mortality, as well as potential interactions among proposed environmental, host, and agent determinants of disease. A clear, invariant relationship between a single agent and field outbreaks has not yet been documented, in part due to methodological limitations and practical challenges associated with developing rigorous study designs. Therefore, although there is a need to develop predictive models for outbreaks and validated mitigation strategies, uncertainty remains as to whether outbreaks are due to endemic or recently introduced agents. Consequently, absence of established and universal explanations for outbreaks contributes to conflict among wildlife and livestock stakeholders over land use and management practices. This example illustrates the challenge of developing comprehensive models for understanding and managing wildlife diseases in complex biological and sociological environments.

  5. Mixed field radiation modification of polyurethanes based on castor oil

    International Nuclear Information System (INIS)

    Mortley, A.; Bonin, H.W.; Bui, V.T.

    2006-01-01

    Polyurethane is among the polymers and polymer-based composite materials being investigated at the Royal Military College of Canada for the fabrication of leak-tight containers for the long-term disposal of radioactive waste. Due to the long aliphatic chain of the castor oil component of polyurethane, thermal curing of castor oil based polyurethane (COPU) is limited by increasing polymer viscosity. To enhance further crosslinking, COPUs were subjected to a range of doses (0.0 - 3.0 MGy) produced by the mixed ionizing radiation field of a SLOWPOKE-2 research nuclear reactor. The tensile mechanical properties of castor oil based polyurethanes (COPU), unirradiated and irradiated, were characterized by mechanical tensile tests. Increases in mechanical strength due to radiation-induced crosslinking and limitations of thermal curing were confirmed by tensile tests and changing 13 C-NMR and FTIR spectra. (author)

  6. Mixed field radiation modification of polyurethanes based on castor oil

    Energy Technology Data Exchange (ETDEWEB)

    Mortley, A.; Bonin, H.W.; Bui, V.T. [Royal Military College of Canada, Dept. of Chemistry and Chemical Engineering, Kingston, Ontario (Canada)]. E-mail: aba.mortley@rmc.ca

    2006-07-01

    Polyurethane is among the polymers and polymer-based composite materials being investigated at the Royal Military College of Canada for the fabrication of leak-tight containers for the long-term disposal of radioactive waste. Due to the long aliphatic chain of the castor oil component of polyurethane, thermal curing of castor oil based polyurethane (COPU) is limited by increasing polymer viscosity. To enhance further crosslinking, COPUs were subjected to a range of doses (0.0 - 3.0 MGy) produced by the mixed ionizing radiation field of a SLOWPOKE-2 research nuclear reactor. The tensile mechanical properties of castor oil based polyurethanes (COPU), unirradiated and irradiated, were characterized by mechanical tensile tests. Increases in mechanical strength due to radiation-induced crosslinking and limitations of thermal curing were confirmed by tensile tests and changing {sup 13}C-NMR and FTIR spectra. (author)

  7. Heterogeneous catalysis afford biodiesel of babassu, castor oil and blends

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Lee M.G. de; Abreu, Wiury C. de; Silva, Maria das Gracas de O. e; Matos, Jose Milton E. de; Moura, Carla V.R. de; Moura, Edmilson M. de, E-mail: mmoura@ufpi.edu.br [Universidade Federal do Piaui (UFPI), Teresina, PI (Brazil). Departamento de Quimica; Lima, Jose Renato de O.; Oliveira, Jose Eduardo de [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP/IQ/CEMPEQC), Araraquara, SP (Brazil). Instituto de Quimica. Centro de Monitoramento e Pesquisa da Qualidade de Combustiveis, Biocombustiveis, Petroleo e Derivados

    2013-04-15

    This work describes the preparation of babassu, castor oil biodiesel and mixtures in various proportions of these oils, using alkaline compounds of strontium (SrCO{sub 3} + SrO + Sr (OH){sub 2}) as heterogeneous catalysts. The mixture of oils of these oleaginous sources was used in the production of biodiesel with quality parameters that meet current legislation. The catalyst was characterized by X-ray diffractometry (XDR), physisorption of gas (BET method), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDX) and Fourier transform infrared spectroscopy (FTIR). The viscometric technique was used to monitor the optimization.The transesterification reactions performed using strontium compounds reached conversion rates of 97.2% babassu biodiesel (BB), 96.4% castor oil biodiesel (COB) and 95.3% Babassu/Castor Oil Biodiesel 4:1 (BBCO41). (author)

  8. Heterogeneous catalysis afford biodiesel of babassu, castor oil and blends

    International Nuclear Information System (INIS)

    Carvalho, Lee M.G. de; Abreu, Wiury C. de; Silva, Maria das Gracas de O. e; Matos, Jose Milton E. de; Moura, Carla V.R. de; Moura, Edmilson M. de; Lima, Jose Renato de O.; Oliveira, Jose Eduardo de

    2013-01-01

    This work describes the preparation of babassu, castor oil biodiesel and mixtures in various proportions of these oils, using alkaline compounds of strontium (SrCO 3 + SrO + Sr (OH) 2 ) as heterogeneous catalysts. The mixture of oils of these oleaginous sources was used in the production of biodiesel with quality parameters that meet current legislation. The catalyst was characterized by X-ray diffractometry (XDR), physisorption of gas (BET method), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDX) and Fourier transform infrared spectroscopy (FTIR). The viscometric technique was used to monitor the optimization.The transesterification reactions performed using strontium compounds reached conversion rates of 97.2% babassu biodiesel (BB), 96.4% castor oil biodiesel (COB) and 95.3% Babassu/Castor Oil Biodiesel 4:1 (BBCO41). (author)

  9. CASTOR: Cathode/Anode Satellite Thruster for Orbital Repositioning

    Science.gov (United States)

    Mruphy, Gloria A.

    2010-01-01

    The purpose of CASTOR (Cathode/Anode Satellite Thruster for Orbital Repositioning) satellite is to demonstrate in Low Earth Orbit (LEO) a nanosatellite that uses a Divergent Cusped Field Thruster (DCFT) to perform orbital maneuvers representative of an orbital transfer vehicle. Powered by semi-deployable solar arrays generating 165W of power, CASTOR will achieve nearly 1 km/s of velocity increment over one year. As a technology demonstration mission, success of CASTOR in LEO will pave the way for a low cost, high delta-V orbital transfer capability for small military and civilian payloads in support of Air Force and NASA missions. The educational objective is to engage graduate and undergraduate students in critical roles in the design, development, test, carrier integration and on-orbit operations of CASTOR as a supplement to their curricular activities. This program is laying the foundation for a long-term satellite construction program at MIT. The satellite is being designed as a part of AFRL's University Nanosatellite Program, which provides the funding and a framework in which student satellite teams compete for a launch to orbit. To this end, the satellite must fit within an envelope of 50cmx50cmx60cm, have a mass of less than 50kg, and meet stringent structural and other requirements. In this framework, the CASTOR team successfully completed PDR in August 2009 and CDR in April 2010 and will compete at FCR (Flight Competition Review) in January 2011. The complexity of the project requires implementation of many systems engineering techniques which allow for development of CASTOR from conception through FCR and encompass the full design, fabrication, and testing process.

  10. Laser-fabricated castor oil-capped silver nanoparticles.

    Science.gov (United States)

    Zamiri, Reza; Zakaria, Azmi; Abbastabar, Hossein; Darroudi, Majid; Husin, Mohd Shahril; Mahdi, Mohd Adzir

    2011-01-01

    Silver nanoparticles were fabricated by ablation of a pure silver plate immersed in castor oil. A Nd:YAG-pulsed Q-switch laser with 1064-nm wavelength and 10-Hz frequency was used to ablate the plate for 10 minutes. The sample was characterized by ultraviolet-visible, atomic absorption, Fourier transform-infrared spectroscopies, and transmission electron microscopy. The results of the fabricated sample showed that the nanoparticles in castor oil were about 5-nm in diameter, well dispersed, and showed stability for a long period of time.

  11. Synthesis of carbon nanotubes using natural carbon precursor: Castor oil

    Science.gov (United States)

    Raziah, A. Z.; Junizah, A. R.; Saifuddin, N.

    2012-09-01

    Castor oil has long been an article of commerce due to its versatility as it is widely used as a starting material for many industrial chemical products because of its unique structure. In this study, carbon nanotubes has been synthesized by thermal decomposition of castor oil in nitrogen atmosphere at 300-400δC using custom-made microwave processing unit. The precursor material was catalyzed by iron clusters originating from the addition of ferrocene. The morphology and characterization of the CNTs were studied and discussed by transmission electron microscopy (TEM).

  12. Hard X-ray studies on the Castor tokamak

    International Nuclear Information System (INIS)

    Mlynar, J.

    1990-04-01

    The electron runaway processes in tokamaks are discussed with regard to hard X radiation measurements. The origin and confinement of runaway electrons, their bremsstrahlung spectra and the influence of lower hybrid current drive on the distribution of high-energy electrons are analyzed for the case of the Castor tokamak. The hard X-ray spectrometer designed for the Castor tokamak is also described and preliminary qualitative results of hard X-ray measurements are presented. The first series of integral measurements made it possible to map the azimuthal dependence of the hard X radiation

  13. Characterization and biological activity of Solidago canadensis complex.

    Science.gov (United States)

    Šutovská, M; Capek, P; Kocmálová, M; Fraňová, S; Pawlaczyk, I; Gancarz, R

    2013-01-01

    Polyphenolic-polysaccharide-protein complex has been isolated from flowers of Solidago canadensis L. by hot alkaline extraction procedure. Compositional analyses of S canadensis complex revealed the presence of carbohydrates (43 wt%), protein (27 wt%), phenolics (12 wt%), uronic acids (10 wt%) and inorganic material (8 wt%). The carbohydrate part was rich in neutral sugars (81 wt%) while uronids were determined in lower amount (19 wt%). Monosaccharide analysis of carbohydrate part revealed the presence of five main sugar components, i.e. rhamnose (~23 wt%), arabinose (~20 wt%), uronic acids (~19 wt%), galactose (~17 wt%) and glucose (~14 wt%), and indicated thus the presence of rhamnogalacturonan and arabinogalactan in S. canadensis complex. HPLC analysis of complex showed one single peak of molecule mass at 11.2 kDa. Antitussive activity tests, performed in three doses of Solidago complex, showed the reduction of the number of cough efforts in the dose-dependent manner. Higher doses (50 and 75 mg/kg b.w.) were shown to be by 15 and 20% more effective than that of lower one (25mg/kg b.w.). However, the antitussive effect of the highest dose (75 mg/kg b.w.) was by 10% lower in comparison with that of codeine, the strongest antitussive agent. Besides, the highest dose of the complex (75 mg/kg b.w.) significantly decreased values of specific airways resistance and their effect remained longer as that of salbutamol, a representative of classic antiasthmatic drugs. Copyright © 2012 Elsevier B.V. All rights reserved.

  14. Sanguinaria canadensis: Traditional Medicine, Phytochemical Composition, Biological Activities and Current Uses

    Directory of Open Access Journals (Sweden)

    Andrew Croaker

    2016-08-01

    Full Text Available Sanguinaria canadensis, also known as bloodroot, is a traditional medicine used by Native Americans to treat a diverse range of clinical conditions. The plants rhizome contains several alkaloids that individually target multiple molecular processes. These bioactive compounds, mechanistically correlate with the plant’s history of ethnobotanical use. Despite their identification over 50 years ago, the alkaloids of S. canadensis have not been developed into successful therapeutic agents. Instead, they have been associated with clinical toxicities ranging from mouthwash induced leukoplakia to cancer salve necrosis and treatment failure. This review explores the historical use of S. canadensis, the molecular actions of the benzophenanthridine and protopin alkaloids it contains, and explores natural alkaloid variation as a possible rationale for the inconsistent efficacy and toxicities encountered by S. canadensis therapies. Current veterinary and medicinal uses of the plant are studied with an assessment of obstacles to the pharmaceutical development of S. canadensis alkaloid based therapeutics.

  15. Sanguinaria canadensis: Traditional Medicine, Phytochemical Composition, Biological Activities and Current Uses.

    Science.gov (United States)

    Croaker, Andrew; King, Graham J; Pyne, John H; Anoopkumar-Dukie, Shailendra; Liu, Lei

    2016-08-27

    Sanguinaria canadensis, also known as bloodroot, is a traditional medicine used by Native Americans to treat a diverse range of clinical conditions. The plants rhizome contains several alkaloids that individually target multiple molecular processes. These bioactive compounds, mechanistically correlate with the plant's history of ethnobotanical use. Despite their identification over 50 years ago, the alkaloids of S. canadensis have not been developed into successful therapeutic agents. Instead, they have been associated with clinical toxicities ranging from mouthwash induced leukoplakia to cancer salve necrosis and treatment failure. This review explores the historical use of S. canadensis, the molecular actions of the benzophenanthridine and protopin alkaloids it contains, and explores natural alkaloid variation as a possible rationale for the inconsistent efficacy and toxicities encountered by S. canadensis therapies. Current veterinary and medicinal uses of the plant are studied with an assessment of obstacles to the pharmaceutical development of S. canadensis alkaloid based therapeutics.

  16. Neglected zoonotic helminths: Hymenolepis nana, Echinococcus canadensis and Ancylostoma ceylanicum.

    Science.gov (United States)

    Thompson, R C A

    2015-05-01

    The majority of helminth parasites that are considered by WHO to be the cause of 'neglected diseases' are zoonotic. In terms of their impact on human health, the role of animal reservoirs and polyparasitism are both emerging issues in understanding the epidemiology of a number of these zoonoses. As such, Hymenolepis (Rodentolepis) nana, Echinococcus canadensis and Ancylostoma ceylanicum all qualify for consideration. They have been neglected and there is increasing evidence that all three parasite infections deserve more attention in terms of their impact on public health as well as their control. Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  17. [Allelopathic interactions between invasive plant Solidago canadensis and native plant Phragmites australis].

    Science.gov (United States)

    Li, Yu-Zhe; Fan, Jiang-Wen; Yin, Xin; Yang, En-Yi; Wei, Wei; Tian, Zhi-Hui; Da, Liang-Jun

    2011-05-01

    Taking the seeds of invasive plant Solidago canadensis and native plant Phragmites australis from their mono- and co-dominant communities as allelopathic acceptors, this paper analyzed the differences in the seed germination rate and sprout length after treated with five level (12.5, 25, 50, 100, and 200 mg x mL(-1)) S. canadensis and P. australis extracts, aimed to understand the allelopathic interactions between the two species. The 1000-grain weight and seed germination rate under distilled water treatment of the two species in co-dominated community were greater than those in mono-dominant community. Low level (12.5 and 25 mg x mL(-1)) S. canadensi extracts slightly promoted the seed germination rates of S. canadensis in both mono- and co-dominant communities, but high level (50, 100, and 200 mg x mL(-1)) S. canadensi extracts had strong inhibition effect, especially for the S. canadensis in co-dominated community. No significant patterns were observed about the effects of P. australis extract on S. canadensis seed germination. The sprout length of S. canadensis seeds in both mono- and co-dominant communities decreased with increasing level of S. canadensis extract, but decreased in a fluctuation way with increasing level of P. australis extract. After treated with the extracts of P. australis or S. canadensis, the seed germination rate of P. australis in mono-dominant community was significantly greater than that in co-dominant community (P < 0.05), but there was no significant difference between these two extracts.

  18. Comparative Analysis of Flame Characteristics of Castor Oil and ...

    African Journals Online (AJOL)

    The flame characteristics of castor oil based foam and that of polyether foam impregnated with inorganic flame retardants (FR) were investigated. The polyether foams were impregnated with measured concentration of Antimony trioxide and Sodium bromide, Ammonium dihydrogen orthophosphate, Diammonium hydrogen ...

  19. GNS Castor V/21 Headspace Gas Sampling 2014

    Energy Technology Data Exchange (ETDEWEB)

    Winston, Philip Lon [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-01-01

    Prior to performing an internal visual inspection, samples of the headspace gas of the GNS Castor V/21 cask were taken on June 12, 2014. These samples were taken in support of the CREIPI/Japanese nuclear industry effort to validate fuel integrity without visual inspection by measuring the 85Kr content of the cask headspace

  20. Evaluation of castor oil samples for potential toxin contamination

    Science.gov (United States)

    Castor oil and its derivatives are widely used as a chemical feedstock for production of lubricants and greases, and for engineering plastics, plasticizers and surfactants. They also have wide application in consumer goods such as lipstick, deodorants and medicinal products. Due to concerns about th...

  1. Growth response of Ricinus communis L (castor oil) in spent ...

    African Journals Online (AJOL)

    The growth response of castor oil plant, Ricinus communis, in spent lubricating oil (SLO) was investigated using 1-6% w/w SLO and a control. The result showed that highest percent germination of approximately 92, was obtained in control and the least in 5% w/w. The early germination obtained in this study was significant ...

  2. Interpenetrating polymer networks based on polyol modified castor ...

    Indian Academy of Sciences (India)

    Interpenetrating polymer networks (IPNs) of glycerol modified castor oil polyurethane (GC–PU) and poly[2-hydroxyethylmethacrylate] (PHEMA) were synthesized using benzoyl peroxide as initiator and N,N-methylene bis acrylamide as crosslinker. GC–PU/PHEMA interpenetrating polymer networks were obtained by ...

  3. Composition of acylglycerols in castor oil and their biosynthetic pathway

    Science.gov (United States)

    Castor oil has many industrial uses, such as the manufacture of aviation lubricant, plastics, paints, coatings, and cosmetics, because of its high content of ricinoleate (hydroxy fatty acid). We have identified many molecular species of acylglycerols using electrospray ionization mass spectrometry o...

  4. Develop a New Lesquerella fendleri Crop for Castor Oil Production

    Science.gov (United States)

    Seed oil of Lesquerella fendleri contains a valuable hydroxy fatty acid (HFA), lesquerolic acid (20:1OH). The conventional source of HFA is ricinoleic acid (18:1OH) from castor seeds. Ricinoleic acid and its derivatives are used as raw materials for numerous industrial products, such as lubricants, ...

  5. CASTOR: Normal-mode analysis of resistive MHD plasmas

    NARCIS (Netherlands)

    Kerner, W.; Goedbloed, J. P.; Huysmans, G. T. A.; Poedts, S.; Schwarz, E.

    1998-01-01

    The CASTOR (complex Alfven spectrum of toroidal plasmas) code computes the entire spectrum of normal-modes in resistive MHD for general tokamak configurations. The applied Galerkin method, in conjunction with a Fourier finite-element discretisation, leads to a large scale eigenvalue problem A (x)

  6. Allelopathic effect of aqueous extract of fresh leaf castor beans ...

    African Journals Online (AJOL)

    Rukevwe S. Abraka

    2016-12-07

    Dec 7, 2016 ... In this study, the allelopathic effect of aqueous extract from fresh leaves of castor .... triturated with electric blender and sieved for the extract preparation. .... (A), Leaf fresh mass; (B), root fresh mass; (C), stem diameter; (D), aerial part length. .... Naphthoquinones as allelochemical triggers of programmed cell.

  7. Diversity of castor ( Ricinus communis L.) in Ethiopia | Alemaw ...

    African Journals Online (AJOL)

    An experiment was carried out to assess the diversity of castor germplasm in Ethiopia. A total of 102 accessions, one elite genotype and two standard varieties were characterized at Melkassa and Arsi Negelle, in the Central Rift Valley of Ethiopia using 12 traits for one during 2013 main season. Analysis of variance ...

  8. Inheritance of Plant Height in two Ethiopian Castor Varieties

    African Journals Online (AJOL)

    The green revolution in Asia was led by the semi dwarf varieties of wheat and rice ... Castor (Racinnus communis L.) is an industrial non edible oil seed that originated in East ... At the fourth generation single plants from each inbred line were.

  9. Experience with the loading and transport of fuel assembly transport casks, including CASTOR casks, and the radiation exposure of personnel

    International Nuclear Information System (INIS)

    Bentele, W.; Kinzelmann, T.

    1999-01-01

    In 1997 and 1998, six spent fuel assembly transports started from the nuclear power plant Gemeinschaftskernkraftwerk Neckar (GKN), using CASTOR-V19 casks. Professor Kuni of Marburg University challenged the statement made by the German Federal Office for Radiation Protection (Bundesamt fuer Strahlenschutz (BfS)) based on accepted scientific knowledge, according to which so-called CASTOR transports present no risk, either to the population or to the escorting police units. This paper shows that the collective dose during the loading of the CASTOR casks amounted to 4.5 mSv (gamma and neutrons) per cask at the most, and that the maximum individual dose amounted to 0.26 mSv. In addition to these doses, the collective dose during handling and transport must be considered: this amounted to 0.35 mSv (gamma and neutrons). The dose to the police escort was -2 (limit for surface contamination), presented degrees of contamination >4 Bq cm -2 upon reaching the Valognes/Cogema terminal. However, transport casks coming from French plants also revealed degrees of contamination >4 Bq cm -2 , as well as 'hot spots'. No such contamination was found on NTL 11 casks transported from the GKN to Sellafield. Neither was any increased contamination found upon the arrival of CASTOR-V19 casks transported from GKN to Gorleben or Ahaus. The partially sensationalist media reports were inversely proportional to the actual radiological relevance of the matter. The German Commission on Radiation Protection (SSK) confirmed that the radiological effect of such contaminated spent fuel transports is negligible. (author)

  10. Passive transfer in domestic and bighorn lambs Total IgG in ewe sera and colostrum and serum IgG kinetics in lambs following colostrum ingestion are similar in domestic sheep and bighorn sheep (ovis aries and ovis canadensis)

    Science.gov (United States)

    Pneumonia is a population-limiting disease of bighorn sheep (BHS; Ovis canadensis) and a recognized disease entity in domestic sheep (DS; Ovis aries) worldwide. Respiratory disease in BHS lambs can persist for years after all-age outbreaks, resulting in suppressed lamb recruitment. It has been sugge...

  11. Demographic consequences of greater clonal than sexual reproduction in Dicentra canadensis.

    Science.gov (United States)

    Lin, Chia-Hua; Miriti, Maria N; Goodell, Karen

    2016-06-01

    Clonality is a widespread life history trait in flowering plants that may be essential for population persistence, especially in environments where sexual reproduction is unpredictable. Frequent clonal reproduction, however, could hinder sexual reproduction by spatially aggregating ramets that compete with seedlings and reduce inter-genet pollination. Nevertheless, the role of clonality in relation to variable sexual reproduction in population dynamics is often overlooked. We combined population matrix models and pollination experiments to compare the demographic contributions of clonal and sexual reproduction in three Dicentra canadensis populations, one in a well-forested landscape and two in isolated forest remnants. We constructed stage-based transition matrices from 3 years of census data to evaluate annual population growth rates, λ. We used loop analysis to evaluate the relative contribution of different reproductive pathways to λ. Despite strong temporal and spatial variation in seed set, populations generally showed stable growth rates. Although we detected some pollen limitation of seed set, manipulative pollination treatments did not affect population growth rates. Clonal reproduction contributed significantly more than sexual reproduction to population growth in the forest remnants. Only at the well-forested site did sexual reproduction contribute as much as clonal reproduction to population growth. Flowering plants were more likely to transition to a smaller size class with reduced reproductive potential in the following year than similarly sized nonflowering plants, suggesting energy trade-offs between sexual and clonal reproduction at the individual level. Seed production had negligible effects on growth and tuber production of individual plants. Our results demonstrate that clonal reproduction is vital for population persistence in a system where sexual reproduction is unpredictable. The bias toward clonality may be driven by low fitness returns

  12. Identification of minor acylglycerols less polar than triricinolein in castor oil by mass spectrometry and the proposed biosynthetic pathway of castor oil

    Science.gov (United States)

    Ricinoleate (OH18:1), a monohydroxy fatty acid, has many industrial uses such as the manufacture of biodegradable plastics, nylon, plasticizers, lubricants, cosmetics and paints. Castor oil is the only commericial source of ricinoleate which occurs as triacylglycerols. Triacylglycerols in castor oil...

  13. SURVEY OF GROSS AND HISTOPATHOLOGIC FINDINGS IN TWO WINTERING SUBPOPULATIONS OF SANDHILL CRANES ( ANTIGONE CANADENSIS).

    Science.gov (United States)

    Hensel, Martha; Bertram, Miranda; Rech, Raquel; Hamer, Gabriel L; Hamer, Sarah A

    2018-01-01

    :  Sandhill Cranes ( Antigone canadensis) of the midcontinent population (MCP) and Rocky Mountain population (RMP) are migratory game birds with stable populations that travel between Canada and the southern US and Mexico. In the winters of 2012-14, we performed gross and histologic examinations of 43 hunter-harvested Sandhill Cranes in Texas (MCP) and New Mexico (RMP) to assess the impact of disease on populations. Lesions were significantly more common in the MCP relative to the RMP, likely reflecting differential environmental exposure to pathogens and parasites. Grossly, liver nodules and esophageal granulomas were present in 8-39% of birds. In feces from over half of birds, we found coccidian oocysts with mitochondrial gene sequences identical to those of Eimeria gruis and Eimeria reichenowi previously obtained from sympatric Whooping Cranes ( Grus americana). Over one-quarter of birds had liver and cardiac lesions suggestive of disseminated visceral coccidiosis. We documented proliferative colitis due to Cryptosporidium in a wild Sandhill Crane. Additionally, several endoparasites were found in histologic sections from several cranes, including a bird with respiratory trematodiasis and two birds with Tetrameres sp. in the proventriculus associated with ductal ectasia. In addition to describing lesions and parasites that impact Sandhill Crane health, these pathology data may also be relevant for the conservation of endangered Whooping Cranes using a surrogate species approach.

  14. The effect of oral castor oil on the disposition of methyprylon in intoxicated dogs.

    Science.gov (United States)

    Gwilt, P R; Pankaskie, M C; Mitala, J J

    1982-07-01

    Clinical observations indicate that large oral doses of castor oil are effective in reducing the time of coma resulting from acute intoxication with lipophilic drugs. It has been further suggested that the rate of removal of these drugs from the body is increased by castor oil. In order to investigate the effect of castor oil on the disposition of lipophilic drugs, five dogs were given toxic doses of methyprylon by intravenous infusion. Each dog was treated with a large oral dose of castor oil in a cross-over fashion. No significant difference was observed in the sleep times of the dogs treated with castor oil, or in the methyprylon pharmacokinetics compared to controls. It was concluded that castor oil does not affect the disposition of methyprylon.

  15. Castor and jatropha oils: production strategies – A review

    Directory of Open Access Journals (Sweden)

    Lago Regina C.A.

    2009-07-01

    Full Text Available The Brazilian bioenergy matrix is based on four platforms: ethanol, energy forests, residues and co-products and biodiesel. The food-energy dichotomy in the use of edible oils is one factor which has stimulated the search for non-edible oleaginous energy crops, such as many native palms. By the year 2000 Brazil had an annual deficit of 80 thousand tons of castor oil, making necessary to import oil from China and India. After a strong debate the National Program on Biodiesel Production (NPBP was launched by December 2004. After an initial excessive enthusiasm, small producers being focused in the program, a more mature and realistic planning is undertaken. Production in semi arid lands is being stimulated, mainly castor (Ricinus communis and Jatropha (Jatropha curcas. Apart from belonging to the same botanical family (Euphorbiaceae, both plants are well resistant to poor soils. Castor plant is well adapted to practically the whole country, except for some extreme areas (too low water availability or too much rain. Castor keeps being an alternative for the semi arid region but much more technology is requested to make it largely exploited. Following the petroleum crisis of 1980’s an ambitious research program on Jatropha curcas was initiated, later on discontinued and presently retaken by Embrapa and some Universities. Progress is slower than in the case of Ricinus communis. The first agronomical observations confirmed low productivity, problems with pests and diseases, high harvesting costs etc. Some strategic factors should be considered for the production of castor and Jatropha oils: 1. Production of raw materials; 2. Production of oils; 3. Detoxification and value aggregation to the extraction cakes and residues. Regarding raw material production, it is necessary a strong, long term research program on genetic breeding (short cycle varieties, with high productivity and allowing a sole harvesting, soil fertility, pest control, domestication

  16. Preparation and research on properties of castor oil as a diesel fuel additive

    OpenAIRE

    Nurbakhit Imankulov

    2012-01-01

    The research shows an opportunity of preparing biodiesel fuel on the basis of local diesel fuel and the bioadditive - castor oil. Limiting optimum concentration of introduction of the bioadditive equal was established as 5% mass ratio. The castor oil released from seeds of Palma Christi grown on experimental field. All physical and chemical characteristics of the oil including IR-spectra were determined. Operating conditions of castor oil introduction (temperature, solubility, concentra-tion,...

  17. Caracterización fármaco-toxicológica de la planta medicinal Sambucus nigra subsp. canadensis (L. R. Bolli Pharmaceutical-toxicological characterization of medicinal plant Sambucus nigra subsp. canadensis (L. R. Bolli

    Directory of Open Access Journals (Sweden)

    Oneyda Clapé Laffita

    2011-12-01

    Full Text Available El objetivo de la monografía consistió en la caracterización fármaco-toxicológica de Sambucus nigra subsp. canadensis (L. R. Bolli, planta tradicional cubana con actividad antiinflamatoria, entre otras. Se presentan aspectos farmacológicos como composición fitoquímica, propiedades atribuidas popularmente y comprobadas científicamente, posología, reacciones adversas, advertencias, interacciones, toxicidad, precauciones y contraindicaciones. Se concluye que con la monografía fármaco-toxicológica de esta planta medicinal, se dispone de una completa información, como documentación indispensable para actualizar las bases de datos nacionales e internacionales al respecto, con vistas a desarrollar nuevos fitofármacos, en especial antiinflamatorios con bajo potencial de efectos adversos.The objective of the monograph was the pharmacological and toxicological characterization of Sambucus nigra subspecies canadensis(L. R. Bolli, a Cuban traditional plant with anti-inflammatory action, among others. Several pharmacological aspects such as phytochemical composition; scientifically tested properties attributed by the population; posology; adverse reactions; warnings; interactions; toxicity; precautions and counterindications were presented. It was concluded that the pharmaceutical and toxicological monograph of this plant provides full information as indispensable documentation to update national and international databases, with a view to developing new pharmaceuticals, particularly anti-inflammatory drugs with low adverse effect potential.

  18. Test of the quasi-optical grill for lower hybrid current drive on the CASTOR tokamak

    International Nuclear Information System (INIS)

    Klima, R.; Pavlo, P.; Preinhaelter, J.; Stoeckel, J.; Zacek, F.; Jakubka, K.; Kletecka, P.; Kryska, L.

    1994-03-01

    Feasibility studies of a new diffraction structure for launching lower hybrid waves into a tokamak plasma - of the microwave quasi-optical grill - are reported. The main parameters of the grill designed for the CASTOR tokamak are summarized, and results of preliminary radiation pattern measurements of a non-optimized model antenna are presented. The influence of a relatively great curvature of the plasma surface in the CASTOR tokamak is discussed and the ray tracing of the launched lower-hybrid wave in the actual CASTOR plasma is shown. Finally, the results of probe measurements of the CASTOR plasma core are given. (J.U.) 17 figs., 9 refs

  19. Seasonal dynamics of Co60 accumulation by Elodea canadensis Rich

    International Nuclear Information System (INIS)

    Bochenin, V.F.; Chebotina, M.Ya.

    1975-01-01

    The seasonal dynamics of Co 60 accumulation by one of the most widely distributed fresh-water plants, elodea (Elodea canadensis Rich), were studied. Accumulation was shown to vary with the season. A very low coefficient of accumulation (500-700 units) was typical for the summer period (June to August). It increased in the fall, reached its highest values (3500-4000) in mid-winter (January), and dropped sharply in the spring. Radioisotope concentrations in the plant varied similarly. The cumulative capacity of plants for Co 60 may vary by a factor of 6 to 7 during the year. It is suggested that the seasonal changes in Co 60 accumulation may be caused by both differences in the physiological state of the plants at different times of the year, and by seasonal variations in the hydrochemical regime of the water reservoir. Experiments were done to clarify which of these mechanisms is the determining factor. (V.A.P.)

  20. Pharmacognostic and physicochemical evaluation of homoeopathic drug: Erigeron canadensis L.

    Directory of Open Access Journals (Sweden)

    Padma Rao Pogaku

    2017-01-01

    Full Text Available Background: Erigeron canadensis L. is an erect annual herb belonging to the family Asteraceae. Aerial parts are used in Homoeopathy for bruises, cough, dysuria, gonorrhea, haemorrhages, haemorrhoids, spermatorrhea, and wounds. Objective: The pharmacognostic and physicochemical studies have been carried out to facilitate the use of correct species and lay down standards of raw drug materials. Materials and Methods: Pharmacognostic studies of the leaf and stem of authentic samples of E. canadensis L. have been carried out. Physicochemical parameters of the raw drug include extractive values, ash value, and formulation; besides weight per mL, total solids, and alcohol content, high-performance thin layer chromatography (HPTLC and ultraviolet (UV studies are given. Results: Epidermal cells often possess crystals of calcium oxalate. Stomata are anomocytic, anisocytic, and tetracytic types. Trichomes are uniseriate and conical in structure. The mid vein in transection is flat on adaxial and is ribbed toward abaxial, with a secretory cavity beneath the central vascular bundle. Stem in transection is round. The vascular tissue is made of several vascular bundles in a ring. Crystals of calcium oxalate occur in the epidermis, cortex, and pith of stem. In mature stem, secondary xylem is well developed with a reduced phloem. The determined physicochemical data, namely, extractive values, ash values, and preparation of for raw drug and weight per mL, total solids, and alcohol content besides UV and HPTLC profile for finished product are provided. Conclusions: The presented morphoanatomical features along with powder microscopic and organoleptic characters and physicochemical data are diagnostic to establish the standards for ensuring quality and purity of the drug.

  1. pre-germination treatments in castor seeds, cultivar IAC 226

    International Nuclear Information System (INIS)

    Costa Nobre, Danubia Aparecida; Gomes Damascena, Joyce; Marcia, Andreia; Santos de Souza, David; Pereira dos Santos, Marlucia; Rodrigues Pereira, Adriana; Goncalves Pereira, Cassio

    2013-01-01

    The present study aimed to evaluate the efficiency of different pre-germination treatments in castor beans, IAC 226. The experimental design was completely randomized in a factorial 4 x 4 (four temperatures and four immersion times), with four replications. Pre-germination treatments were: immersion in water at room temperature (25 Celsius degrade) and immersion in hot water at temperatures of 60, 70 and 80 Celsius degrade for 2, 4, 6 and 8 minutes. Water content of the seeds was determined before treatments. Before and after each treatment, seeds were subjected to germination test; 20-30 Celsius degrade alternating temperature, determining the percentages of normal and abnormal seedlings, dormant and dead seeds. Independent of time, immersion in 70 Celsius degrade, water was the most efficient treatment for accelerating germination of castor bean cultivar IAC 226.

  2. Production of quartz plates for CMS-CASTOR Experiment

    CERN Document Server

    Adiguzel, Aytul; Ayhan, Aydin; Bakirci, Mustafa Numan; Basegmez, Suzan; Beaumont, Willem; Borras, Kerstin; Campbell, Alan; De Paula Carvalho, W; Çerçi, Salim; De Jesus Damiao, Dilson; Dogangün, O; Dumanoglu, Isa; d'Enterria, David; Erchov, Y; Eskut, Eda; Figueiredo, D; Girgis, Semiray; Göttlicher, P; Gouskos, Loukas; Gurpinar, Emine; Hos, Ilknur; Katkov, Igor; Katsas, Panagiotis; Khein, Lev; Knutsson, Albert; Kuznetsov, Andrey; Lebeau, Michel; Van Mechelen, Pierre; Muhl, Carsten; Musienko, Yuri; Ochesanu, Silvia; Onengüt, G; Onengut, G Jr; Ozdemir, Kadri; Panagiotou, Apostolos; Polatoz, A; Ripert, Marion; Shileev, K; Sogut, Kenan; Tiflov, B TaliV; Kayis-Topaksu, A; Uzun, Dilber

    2008-01-01

    Light transmission rate performance of $102$ irradiated quartz samples was measured to select the best quartz plates for CMS-CASTOR calorimeter. All the produced quartz plates were originally used in a previous CERN experiment, DELPHI. Three different doses of $^{60}$Co source were used with the collaboration of PSI (Paul Scherrer Institute, Villigen PSI, Switzerland.) to study the transmission rate performance of the quartz samples after irradiation for different incident light, ranging from $250$ to $700$ nm in $5$ nm increasing steps. All samples show different decrease in the rate with wavelength for different doses. Three different steps were followed before irradiation to find out the best way of cleaning the original DELPHI Cu/Cr tracks on the samples. Results of these measurements presented here correspond to the quartz plates that will be used in one hadronic sector of CASTOR calorimeter until end of 2008. For the full calorimeter new quartz plates will be installed. We also present the light transmi...

  3. Extraction, Characterization and Modification of Castor Seed Oil

    Directory of Open Access Journals (Sweden)

    A. D. MOHAMMED

    2006-01-01

    Full Text Available This paper carried out experimental study, through extraction and characterization of both crude and refined castor oil. Normal hexane was used as solvent for the extraction process. The oil produced was refined through degumming, neutralization and bleaching process using local adsorbent (activated clay. The characterization analysis revealed that tested parameters, which include specific gravity, refractive index, acid value, saponification value and iodine value for both crude and refined castor oil produced, were within the ASTM standard specifications. In fact the iodine value obtained (84.8 for the refined oil indicates that the oil could certainly be used as lubricant, hydraulic break fluid and protecting coatings. The oil was modified via sulphation method to produce Turkey – red oil that was tested on wooden material, paper and cloth. The test revealed that the Turkey – red oil produced is suitable to be used as a good dying agent and polish.

  4. Fallot's tetralogy in a European beaver (Castor fiber).

    Science.gov (United States)

    Wenger, Sandra; Gull, Jessica; Glaus, Tony; Blumer, Serafin; Wimmershoff, Julia; Kranjc, Asja; Steinmetz, Hanspeter; Hatt, Jean-Michel

    2010-06-01

    A 20-mo-old, female, 9-kg European beaver (Castor fiber) presented with apathy, reduced appetite of 3-day duration and a grade 5/6 systolic heart murmur. Thoracic radiographs revealed a diffuse broncho-interstitial pattern suspicious for bronchopneumonia. The echocardiographic findings of a hypertrophied right ventricle, ventricular septal defect with overriding aorta, and infundibular pulmonic stenosis were consistent with Fallot's tetralogy. Even though the bronchopneumonia rather than the congenital cardiac defect was considered of primary importance for the presenting clinical signs, the latter was relevant for the decision not to continue any medical treatment. Both disease processes were confirmed on necropsy. Fallot's tetralogy, European beaver, Castor fiber, heart murmur, ultrasound.

  5. "Castor Oil" - The Culprit of Acute Hair Felting.

    Science.gov (United States)

    Maduri, V Ramya; Vedachalam, Ahalya; Kiruthika, S

    2017-01-01

    Acute hair felting is a rare disorder of scalp hair. In this condition, the hair becomes twisted, entangled as a hard stony mass resembling a bird's nest. Sudden hair matting has been reported earlier in the literature after vigorous use of chemical and herbal shampoos. Plica polonica is a patchy area of hair matting occurring in due course of time in neglected hair or underlying psychiatric illness. This case is interesting as the whole scalp hair matted immediately after using coconut oil and castor oil following washing. Growing long hair and taking oil bath are cultural and religious customs in South India. The high viscosity of castor oil and long hair had contributed to sudden felting of hair. This disorder of hair is irreversible and the hair should be cut off. Acute nature of this disorder will result in a serious psychological impact on the patient and the family.

  6. Biodiesel production from castor oil in Brazil: A difficult reality

    International Nuclear Information System (INIS)

    Silva Cesar, Aldara da; Otavio Batalha, Mario

    2010-01-01

    The Brazilian National Program for Production and Use of Biodiesel (PNPB in Portuguese) has created a huge demand for biodiesel in Brazil. The PNPB is strongly based on social development through the inclusion of family farmers in projects integrated with biodiesel power plants. Among the various oilseeds, castor bean (Ricinus communis L.) was identified as the ideal one to promote social development in the semi-arid region. However, although promising, the mechanisms of the federal program are still insufficient to promote the effective participation of family farmers. This research shows that companies are facing huge problems in implementing contracts with family farmers. It describes and analyzes the functioning dynamics of this agro-production chain. This paper addresses the identification and the discussion of these obstacles, in order to increase the competitiveness of the biodiesel agribusiness chain, based on castor oil social projects in Brazil.

  7. Variations in seed traits of castor ( Ricinus communis ) accessions ...

    African Journals Online (AJOL)

    The physicochemical analysis showed that castor seed and oil had saponification value of 182.9 mg/g, moisture content of 4.4%, acid value of 3.085 mg/g, viscosity of 110.41 cP, pH of 6.11, iodine value of 8.46 mg/g, specific gravity of 0.962 and refractive index of 1.477˚C. The proximate analysis showed that moisture ...

  8. A new phenolic glycoside from the aerial parts of Solidago canadensis.

    Science.gov (United States)

    Zhang, JinSong; Zhang, XinQin; Lei, GuangQing; Li, Bo; Chen, JiaKuan; Zhou, TongShui

    2007-01-01

    A new phenolic glycoside, 2'-hydroxy-4',6'-di-O-beta-D-glucopyranosyl-butyrrophenone (1), was isolated from the aerial parts of Solidago canadensis. The structure was elucidated on the basis of spectroscopic methods.

  9. Effect of copper on growth of an aquatic macrophyte, Elodea canadensis

    Energy Technology Data Exchange (ETDEWEB)

    Mal, T.K.; Adorjan, Peter; Corbett, A.L

    2002-12-01

    Elodea canadensis may be a good biomonitor for copper, but not a good bioaccumulator. - Elodea canadensis has been proposed as a potential biomonitor due to its wide distribution and apparent ability to accumulate pollutants in aquatic ecosystems. We investigated the effects of copper sulfate on growth in E. canadensis to determine its effectiveness as a biomonitor of copper pollution in aquatic systems and whether growth is a suitable index of sub-lethal stress. Copper sulfate significantly slowed or stopped growth at all concentrations (low: 1 ppm, medium: 5 ppm, high: 10 ppm of copper sulfate) used. Final plant drymass was significantly lower in medium and high copper treatments compared with controls. E. canadensis appears to be very sensitive to copper levels, and may be useful as a biomonitor of copper levels in aquatic systems. However, its utility as a bioaccumulator may be limited, because we observed senescence of most leaves in all copper-treated plants following 4 weeks of treatment.

  10. Effect of copper on growth of an aquatic macrophyte, Elodea canadensis

    International Nuclear Information System (INIS)

    Mal, T.K.; Adorjan, Peter; Corbett, A.L.

    2002-01-01

    Elodea canadensis may be a good biomonitor for copper, but not a good bioaccumulator. - Elodea canadensis has been proposed as a potential biomonitor due to its wide distribution and apparent ability to accumulate pollutants in aquatic ecosystems. We investigated the effects of copper sulfate on growth in E. canadensis to determine its effectiveness as a biomonitor of copper pollution in aquatic systems and whether growth is a suitable index of sub-lethal stress. Copper sulfate significantly slowed or stopped growth at all concentrations (low: 1 ppm, medium: 5 ppm, high: 10 ppm of copper sulfate) used. Final plant drymass was significantly lower in medium and high copper treatments compared with controls. E. canadensis appears to be very sensitive to copper levels, and may be useful as a biomonitor of copper levels in aquatic systems. However, its utility as a bioaccumulator may be limited, because we observed senescence of most leaves in all copper-treated plants following 4 weeks of treatment

  11. Effect of copper on castor bean (Ricinus communis L.) growth

    Energy Technology Data Exchange (ETDEWEB)

    Chaves, Lucia Helena Garofalo; Cunha, Tassio Cavalcanti da Silva; Lima, Vinicius Mota; Cabral, Paulo Cesar Pinto; Barros Junior, Genival; Lacerda, Rogerio Dantas de [Universidade Federal de Campina Grande (UAEAg/UFCG), PB (Brazil). Unidade Academica de Engenharia Agricola

    2008-07-01

    Castor beans crop (Ricinus communis L.) is raising attention as an alternative crop for oil and biodiesel production. Despite the mineral fertilization is an important factor for increasing castor yield, few research has been made on this issue, mainly on the use de copper. In order to evaluate the effects of copper on growth of this plant an experiment was carried out in a greenhouse, in Campina Grande, Paraiba State, Brazil, from July to December 2007. The substrate for the pot plants was a 6 mm-sieved surface soil (Neossolo Quartzarenico). The experimental design was a completely randomized with three replications. The treatments were composed of five levels of Cu (0; 1; 2; 3 and 4 mg dm{sup -3}), which were applied at the time of planting. One plant of castor bean, cultivar BRS 188 - Paraguacu, was grown per pot after thinning and was irrigated whenever necessary. Data on plant height, number and length of leaves and stem diameter were measured at 21, 34, 77 and 103 days after planting. Copper levels used, in general, did not affect the plant height, stem diameter and leaf area, however they influenced the leaves and shoot biomass dry mass and the quadratic trend was the best to show the behavior of these. (author)

  12. Trial of an experimental castor oil solution for cleaning dentures.

    Science.gov (United States)

    Andrade, Ingrid Machado de; Andrade, Kelly Machado de; Pisani, Marina Xavier; Silva-Lovato, Cláudia Helena; de Souza, Raphael Freitas; Paranhos, Helena de Freitas Oliveira

    2014-01-01

    Denture hygiene is essential because denture biofilm is involved in oral infections and systemic diseases. Although there are chemical agents available on the market, none of them have ideal properties and research on such products is still necessary. The aim of this study was to evaluate the efficacy of a castor bean (Ricinus communis)-based solution for removing denture biofilm, compared to two traditional products (sodium hypochlorite and alkaline peroxide). Fifty maxillary complete denture wearers were instructed to brush their dentures after meals and to immerse their dentures once a day in the following solutions: Saline (20 min; control), Polident alkaline peroxide (3 min), NaOCl (20 min) and 2% castor oil solution (20 min). Participants used each solution for a period of 7 consecutive days, according to a random sequence. After each period, the internal surfaces of maxillary complete dentures were stained with a disclosing solution (1% neutral red), photographed and the disclosed biofilm was quantified with the aid of specific software. The influence of treatments on results was verified by the Friedman test (α=0.05). Tested solutions presented significant difference (Fr=51.67; pcastor oil presented intermediate results (median: 1.0% and 1.5%, respectively). It can be concluded that the castor oil solution tested in this study was comparable to alkaline peroxide in terms of efficiency in denture biofilm removal.

  13. Low-modulus PMMA bone cement modified with castor oil.

    Science.gov (United States)

    López, Alejandro; Hoess, Andreas; Thersleff, Thomas; Ott, Marjam; Engqvist, Håkan; Persson, Cecilia

    2011-01-01

    Some of the current clinical and biomechanical data suggest that vertebroplasty causes the development of adjacent vertebral fractures shortly after augmentation. These findings have been attributed to high injection volumes as well as high Young's moduli of PMMA bone cements compared to that of the osteoporotic cancellous bone. The aim of this study was to evaluate the use of castor oil as a plasticizer for PMMA bone cements. The Young's modulus, yield strength, maximum polymerization temperature, doughing time, setting time and the complex viscosity curves during curing, were determined. The cytotoxicity of the materials extracts was assessed on cells of an osteoblast-like cell line. The addition of up to 12 wt% castor oil decreased yield strength from 88 to 15 MPa, Young's modulus from 1500 to 446 MPa and maximum polymerization temperature from 41.3 to 25.6°C, without affecting the setting time. However, castor oil seemed to interfere with the polymerization reaction, giving a negative effect on cell viability in a worst-case scenario.

  14. Measurement of quadratic electrogyration effect in castor oil

    Science.gov (United States)

    Izdebski, Marek; Ledzion, Rafał; Górski, Piotr

    2015-07-01

    This work presents a detailed analysis of electrogyration measurement in liquids with the usage of an optical polarimetric technique. Theoretical analysis of the optical response to an applied electric field is illustrated by experimental data for castor oil which exhibits natural optical activity, quadratic electro-optic effect and quadratic electrogyration effect. Moreover, the experimental data show that interaction of the oil with a pair of flat electrodes induces a significant dichroism and natural linear birefringence. The combination of these effects occurring at the same time complicates the procedure of measurements. It has been found that a single measurement is insufficient to separate the contribution of the electrogyration effect, but it is possible on the basis of several measurements performed with various orientations of the polarizer and the analyser. The obtained average values of the quadratic electrogyration coefficient β13 in castor oil at room temperature are from - 0.92 ×10-22 to - 1.44 ×10-22m2V-2 depending on the origin of the oil. Although this study is focused on measurements in castor oil, the presented analysis is much more general.

  15. [Effects of Cuscuta australis parasitism on the growth, reproduction and defense of Solidago canadensis].

    Science.gov (United States)

    Yang, Bei-fen; Du, Le-shan; Li, Jun-min

    2015-11-01

    In order to find out how parasitic Cuscuta australis influences the growth and reproduction of Solidago canadensis, the effects of the parasitism of C. australis on the morphological, growth and reproductive traits of S. canadensis were examined and the relationships between the biomass and the contents of the secondary metabolites were analyzed. The results showed that the parasitism significantly reduced the plant height, basal diameter, root length, root diameter, root biomass, stem biomass, leaf biomass, total biomass, number of inflorescences branches, axis length of inflorescence, and number of inflorescence. In particular, plant height, number of inflorescence and the stem biomass of parasitized S. canadensis were only 1/2, 1/5 and 1/8 of non-parasitized plants, respectively. There was no significant difference of plant height, root length, stem biomass and total biomass between plants parasitized with high and low intensities. But the basal diameter, root volume, leaf biomass, root biomass, the number of inflorescences branches, axis length of inflorescence and number of inflorescence of S. canadensis parasitized with high intensity were significantly lower than those of plants parasitized with low intensity. The parasitism of C. australis significantly increased the tannins content in the root and the flavonoids content in the stem of S. canadensis. The biomass of S. canadensis was significantly negatively correlated with the tannin content in the root and the flavonoids content in the stem. These results indicated that the parasitism of C. australis could inhibit the growth of S. canadensis by changing the resources allocation patterns as well as reducing the resources obtained by S. canadensis.

  16. Sanguinaria canadensis: Traditional Medicine, Phytochemical Composition, Biological Activities and Current Uses

    OpenAIRE

    Croaker, Andrew; King, Graham J.; Pyne, John H.; Anoopkumar-Dukie, Shailendra; Liu, Lei

    2016-01-01

    Sanguinaria canadensis, also known as bloodroot, is a traditional medicine used by Native Americans to treat a diverse range of clinical conditions. The plants rhizome contains several alkaloids that individually target multiple molecular processes. These bioactive compounds, mechanistically correlate with the plant’s history of ethnobotanical use. Despite their identification over 50 years ago, the alkaloids of S. canadensis have not been developed into successful therapeutic agents. Instead...

  17. Use of exposure history to identify patterns of immunity to pneumonia in bighorn sheep (Ovis canadensis.

    Directory of Open Access Journals (Sweden)

    Raina K Plowright

    Full Text Available Individual host immune responses to infectious agents drive epidemic behavior and are therefore central to understanding and controlling infectious diseases. However, important features of individual immune responses, such as the strength and longevity of immunity, can be challenging to characterize, particularly if they cannot be replicated or controlled in captive environments. Our research on bighorn sheep pneumonia elucidates how individual bighorn sheep respond to infection with pneumonia pathogens by examining the relationship between exposure history and survival in situ. Pneumonia is a poorly understood disease that has impeded the recovery of bighorn sheep (Ovis canadensis following their widespread extirpation in the 1900s. We analyzed the effects of pneumonia-exposure history on survival of 388 radio-collared adults and 753 ewe-lamb pairs. Results from Cox proportional hazards models suggested that surviving ewes develop protective immunity after exposure, but previous exposure in ewes does not protect their lambs during pneumonia outbreaks. Paradoxically, multiple exposures of ewes to pneumonia were associated with diminished survival of their offspring during pneumonia outbreaks. Although there was support for waning and boosting immunity in ewes, models with consistent immunizing exposure were similarly supported. Translocated animals that had not previously been exposed were more likely to die of pneumonia than residents. These results suggest that pneumonia in bighorn sheep can lead to aging populations of immune adults with limited recruitment. Recovery is unlikely to be enhanced by translocating naïve healthy animals into or near populations infected with pneumonia pathogens.

  18. Use of exposure history to identify patterns of immunity to pneumonia in bighorn sheep (Ovis canadensis).

    Science.gov (United States)

    Plowright, Raina K; Manlove, Kezia; Cassirer, E Frances; Cross, Paul C; Besser, Thomas E; Hudson, Peter J

    2013-01-01

    Individual host immune responses to infectious agents drive epidemic behavior and are therefore central to understanding and controlling infectious diseases. However, important features of individual immune responses, such as the strength and longevity of immunity, can be challenging to characterize, particularly if they cannot be replicated or controlled in captive environments. Our research on bighorn sheep pneumonia elucidates how individual bighorn sheep respond to infection with pneumonia pathogens by examining the relationship between exposure history and survival in situ. Pneumonia is a poorly understood disease that has impeded the recovery of bighorn sheep (Ovis canadensis) following their widespread extirpation in the 1900s. We analyzed the effects of pneumonia-exposure history on survival of 388 radio-collared adults and 753 ewe-lamb pairs. Results from Cox proportional hazards models suggested that surviving ewes develop protective immunity after exposure, but previous exposure in ewes does not protect their lambs during pneumonia outbreaks. Paradoxically, multiple exposures of ewes to pneumonia were associated with diminished survival of their offspring during pneumonia outbreaks. Although there was support for waning and boosting immunity in ewes, models with consistent immunizing exposure were similarly supported. Translocated animals that had not previously been exposed were more likely to die of pneumonia than residents. These results suggest that pneumonia in bighorn sheep can lead to aging populations of immune adults with limited recruitment. Recovery is unlikely to be enhanced by translocating naïve healthy animals into or near populations infected with pneumonia pathogens.

  19. Hematologic parameters and hemoparasites of nonmigratory Canada geese (Branta canadensis) from Greensboro, North Carolina, USA.

    Science.gov (United States)

    Charles-Smith, Lauren E; Rutledge, M Elizabeth; Meek, Caroline J; Baine, Katherine; Massey, Elizabeth; Ellsaesser, Laura N; DePerno, Christopher S; Moorman, Christopher E; Degernes, Laurel A

    2014-03-01

    Large flocks of wild, nonmigratory Canada geese (Branta canadensis) have established permanent residence throughout the eastern United States and have become a public concern. Few studies have assessed the hematologic parameters for these populations, which could provide useful information for monitoring individual and population health of Canada geese. This study measured the hematologic parameters and detected the presence of hemoparasites from 146 wild, nonmigratory Canada geese in central North Carolina, USA, during their annual molt. The age class, sex, and weight of each bird were recorded at capture. Values for packed cell volume (PCV), estimated white blood cell count, white blood cell differentials, and heterophil: lymphocyte ratios were calculated for each bird. Adults and female geese had higher estimated white blood cell counts compared with juveniles and males, respectively. The PCV increased with weight and age class. Adult geese had higher percentages of heterophils and heterophil: lymphocyte ratios, whereas juvenile geese had higher percentages of lymphocytes. Relative eosinophil counts in adults increased with decreasing bird weight, and relative monocyte counts in juveniles increased with increasing weight. Three percent of geese were infected with species of Hemoproteus blood parasites. Atypical lymphocyte morphology, including pseudopods, split nuclei, and cytoplasmic granules, was observed in 5% of the birds. The hematologic values reported for adult and juvenile nonmigratory Canada geese in this study may serve as reference intervals for ecological studies and veterinary care of wild and captive Canada geese.

  20. Investigation of glyphosate resistance levels and target-site based resistance (TSR) mechanisms in Conyza canadensis (L.) from apple orchards around areas of Bohai seas and Loess Plateau in China.

    Science.gov (United States)

    Mei, Yu; Xu, Yufang; Wang, Shipeng; Qiu, Lihong; Zheng, Mingqi

    2018-04-01

    The resistance levels to glyphosate and target-site based resistance mechanisms in susceptible (S) and resistant (R) Conyza canadensis (L.) populations, which were collected from apple orchards around areas of Bohai seas and Loess Plateau in China, were investigated. Among forty C. canadensis populations, eighteen populations (45%) were still susceptible; fourteen populations (35%) evolved low resistance levels resistance to glyphosate with resistance index (RI) of 2.02 to 3.90. In contrast, eight populations (20%) evolved medium resistance levels with RI of 4.35 to 8.38. The shikimic acid concentrations in R populations were highly negative relative with the glyphosate resistance levels in C. canadensis, the Pearson correlation coefficient was -0.82 treated by glyphosate at 1.8mg/L. Three 5-enoylpyruvylshikimate 3'-phosphate synthase genes (EPSPS1, EPSPS2 and EPSPS3) were cloned in all S and glyphosate-resistant C. canadensis populations. No amino acid substitution was identified at site of 102 and 106 in three EPSPS genes, which were reported to confer glyphosate resistance in other weed species. The relative expression level of EPSPS mRNA in R populations (SD07, LN05, SHX06 and SD09) was 4.5 to 13.2 times higher than in S biotype. The Pearson correlation coefficient between EPSPS expression levels and RI was 0.79, which indicated the over expression of EPSPS mRNA may cause these R populations evolve higher resistance level to glyphosate. Copyright © 2018 Elsevier Inc. All rights reserved.

  1. Mass spectrometry of the lithium adducts of diacylglycerols containing hydroxy FA in castor oil and two normal FA

    Science.gov (United States)

    Castor oil can be used in industry. The molecular species of triacylglycerols containing hydroxy fatty acids (FA) in castor oil have been identified. We report here the identification of twelve diacylglycerols (DAG) containing hydroxy FA in castor oil using positive ion electrospray ionization mass ...

  2. Local extinction and unintentional rewilding of bighorn sheep (Ovis canadensis on a desert island.

    Directory of Open Access Journals (Sweden)

    Benjamin T Wilder

    Full Text Available Bighorn sheep (Ovis canadensis were not known to live on Tiburón Island, the largest island in the Gulf of California and Mexico, prior to the surprisingly successful introduction of 20 individuals as a conservation measure in 1975. Today, a stable island population of ∼500 sheep supports limited big game hunting and restocking of depleted areas on the Mexican mainland. We discovered fossil dung morphologically similar to that of bighorn sheep in a dung mat deposit from Mojet Cave, in the mountains of Tiburón Island. To determine the origin of this cave deposit we compared pellet shape to fecal pellets of other large mammals, and extracted DNA to sequence mitochondrial DNA fragments at the 12S ribosomal RNA and control regions. The fossil dung was 14C-dated to 1476-1632 calendar years before present and was confirmed as bighorn sheep by morphological and ancient DNA (aDNA analysis. 12S sequences closely or exactly matched known bighorn sheep sequences; control region sequences exactly matched a haplotype described in desert bighorn sheep populations in southwest Arizona and southern California and showed subtle differentiation from the extant Tiburón population. Native desert bighorn sheep previously colonized this land-bridge island, most likely during the Pleistocene, when lower sea levels connected Tiburón to the mainland. They were extirpated sometime in the last ∼1500 years, probably due to inherent dynamics of isolated populations, prolonged drought, and (or human overkill. The reintroduced population is vulnerable to similar extinction risks. The discovery presented here refutes conventional wisdom that bighorn sheep are not native to Tiburón Island, and establishes its recent introduction as an example of unintentional rewilding, defined here as the introduction of a species without knowledge that it was once native and has since gone locally extinct.

  3. Local extinction and unintentional rewilding of bighorn sheep (Ovis canadensis) on a desert island

    Science.gov (United States)

    Wilder, Benjamin T.; Betancourt, Julio L.; Epps, Clinton W.; Crowhurst, Rachel S.; Mead, Jim I.; Ezcurra, Exequiel

    2014-01-01

    Bighorn sheep (Ovis canadensis) were not known to live on Tiburón Island, the largest island in the Gulf of California and Mexico, prior to the surprisingly successful introduction of 20 individuals as a conservation measure in 1975. Today, a stable island population of ~500 sheep supports limited big game hunting and restocking of depleted areas on the Mexican mainland. We discovered fossil dung morphologically similar to that of bighorn sheep in a dung mat deposit from Mojet Cave, in the mountains of Tiburón Island. To determine the origin of this cave deposit we compared pellet shape to fecal pellets of other large mammals, and extracted DNA to sequence mitochondrial DNA fragments at the 12S ribosomal RNA and control regions. The fossil dung was 14C-dated to 1476–1632 calendar years before present and was confirmed as bighorn sheep by morphological and ancient DNA (aDNA) analysis. 12S sequences closely or exactly matched known bighorn sheep sequences; control region sequences exactly matched a haplotype described in desert bighorn sheep populations in southwest Arizona and southern California and showed subtle differentiation from the extant Tiburón population. Native desert bighorn sheep previously colonized this land-bridge island, most likely during the Pleistocene, when lower sea levels connected Tiburón to the mainland. They were extirpated sometime in the last ~1500 years, probably due to inherent dynamics of isolated populations, prolonged drought, and (or) human overkill. The reintroduced population is vulnerable to similar extinction risks. The discovery presented here refutes conventional wisdom that bighorn sheep are not native to Tiburón Island, and establishes its recent introduction as an example of unintentional rewilding, defined here as the introduction of a species without knowledge that it was once native and has since gone locally extinct.

  4. Local Extinction and Unintentional Rewilding of Bighorn Sheep (Ovis canadensis) on a Desert Island

    Science.gov (United States)

    Wilder, Benjamin T.; Betancourt, Julio L.; Epps, Clinton W.; Crowhurst, Rachel S.; Mead, Jim I.; Ezcurra, Exequiel

    2014-01-01

    Bighorn sheep (Ovis canadensis) were not known to live on Tiburón Island, the largest island in the Gulf of California and Mexico, prior to the surprisingly successful introduction of 20 individuals as a conservation measure in 1975. Today, a stable island population of ∼500 sheep supports limited big game hunting and restocking of depleted areas on the Mexican mainland. We discovered fossil dung morphologically similar to that of bighorn sheep in a dung mat deposit from Mojet Cave, in the mountains of Tiburón Island. To determine the origin of this cave deposit we compared pellet shape to fecal pellets of other large mammals, and extracted DNA to sequence mitochondrial DNA fragments at the 12S ribosomal RNA and control regions. The fossil dung was 14C-dated to 1476–1632 calendar years before present and was confirmed as bighorn sheep by morphological and ancient DNA (aDNA) analysis. 12S sequences closely or exactly matched known bighorn sheep sequences; control region sequences exactly matched a haplotype described in desert bighorn sheep populations in southwest Arizona and southern California and showed subtle differentiation from the extant Tiburón population. Native desert bighorn sheep previously colonized this land-bridge island, most likely during the Pleistocene, when lower sea levels connected Tiburón to the mainland. They were extirpated sometime in the last ∼1500 years, probably due to inherent dynamics of isolated populations, prolonged drought, and (or) human overkill. The reintroduced population is vulnerable to similar extinction risks. The discovery presented here refutes conventional wisdom that bighorn sheep are not native to Tiburón Island, and establishes its recent introduction as an example of unintentional rewilding, defined here as the introduction of a species without knowledge that it was once native and has since gone locally extinct. PMID:24646515

  5. Synthesis and membrane performance characterization of self-emulsified waterborne nitrocellulose dispersion modified with castor oil

    Energy Technology Data Exchange (ETDEWEB)

    Su, Xiuxia; Zhao, Qingxiao, E-mail: 934481965@qq.com; Zhang, Dan; Dong, Wei

    2015-11-30

    Graphical abstract: - Highlights: • Waterborne nitrocellulose dispersion modified with castor oil (CWNC) was synthesized successfully. • It is a kind of environment-friendly coatings, in which volatile organic content (VOC) is near zero. • Castor oil used as an internal crosslinking agent for WNC improved the properties of the coating. • When the mass fraction of castor oil to total reactants is 7%, emulsion and the coating are of the best comprehensive performance. - Abstract: Waterborne nitrocellulose dispersion modified with castor oil (CWNC) was designed and successfully synthesized by self emulsification and reaction among isophorone diisocyanate (IPDI) trimer, dimethylol propionic acid (DMPA), nitrocellulose (NC) and castor oil (C.O.). The CWNC was characterized by transmission electron microscope (TEM), Fourier transform infrared (FTIR) spectroscopy and thermal gravimetric analysis (TGA), etc. The particle size of CWNC increased with the increase of mass fraction of castor oil to total reactants, ω (C.O.). The morphology of particles is an approximate core–shell structure indicated by TEM. FTIR confirmed that the reactions (i.e. IPDI trimer and castor oil, IPDI trimer and NC) occurred, the NCO groups of IPDI trimer were consumed totally and the backbone of NC was retained. The water contact angle measurements confirmed that introduced castor oil increased hydrophobicity of the film, thereby increasing the contact angle. TGA revealed that the CWNC film had better thermal resistance.

  6. 21 CFR 524.2620 - Liquid crystalline trypsin, Peru balsam, castor oil.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Liquid crystalline trypsin, Peru balsam, castor... NEW ANIMAL DRUGS § 524.2620 Liquid crystalline trypsin, Peru balsam, castor oil. (a)(1) Specifications... delivered to the wound site contains 0.12 milligram of crystalline trypsin, 87.0 milligrams of Peru balsam...

  7. Identification of minor acylglycerols less polar than triricinolein in castor oil by mass spectrometry

    Science.gov (United States)

    Triacylglycerols in castor oil less polar than triricinolein were identified by electrospray ionization – mass spectrometry using the lithium adducts of the acylglycerols from the HPLC fractions of castor oil. Thirty four new molecular species of acylglycerols containing hydroxy fatty acids in casto...

  8. Synthesis and membrane performance characterization of self-emulsified waterborne nitrocellulose dispersion modified with castor oil

    International Nuclear Information System (INIS)

    Su, Xiuxia; Zhao, Qingxiao; Zhang, Dan; Dong, Wei

    2015-01-01

    Graphical abstract: - Highlights: • Waterborne nitrocellulose dispersion modified with castor oil (CWNC) was synthesized successfully. • It is a kind of environment-friendly coatings, in which volatile organic content (VOC) is near zero. • Castor oil used as an internal crosslinking agent for WNC improved the properties of the coating. • When the mass fraction of castor oil to total reactants is 7%, emulsion and the coating are of the best comprehensive performance. - Abstract: Waterborne nitrocellulose dispersion modified with castor oil (CWNC) was designed and successfully synthesized by self emulsification and reaction among isophorone diisocyanate (IPDI) trimer, dimethylol propionic acid (DMPA), nitrocellulose (NC) and castor oil (C.O.). The CWNC was characterized by transmission electron microscope (TEM), Fourier transform infrared (FTIR) spectroscopy and thermal gravimetric analysis (TGA), etc. The particle size of CWNC increased with the increase of mass fraction of castor oil to total reactants, ω (C.O.). The morphology of particles is an approximate core–shell structure indicated by TEM. FTIR confirmed that the reactions (i.e. IPDI trimer and castor oil, IPDI trimer and NC) occurred, the NCO groups of IPDI trimer were consumed totally and the backbone of NC was retained. The water contact angle measurements confirmed that introduced castor oil increased hydrophobicity of the film, thereby increasing the contact angle. TGA revealed that the CWNC film had better thermal resistance.

  9. Methyl esters from vegetable oils with hydroxy fatty acids: Comparison of lesquerella and castor methyl esters

    Science.gov (United States)

    The search for alternative feedstocks for biodiesel as partial replacement for petrodiesel has recently extended to castor oil. In this work, the castor oil methyl esters were prepared and their properties determined in comparison to the methyl esters of lesquerella oil, which in turn is seen as alt...

  10. Acute Toxicity of Castor Oil Bean Extract and Tolerance Level of ...

    African Journals Online (AJOL)

    The experiment was carried out to determine the acute toxicity of raw castor oil bean (Ricinus communis) extract and the tolerance level of raw castor oil bean by broilers. The seeds were ground, defatted with petroleum ether and the residue was subjected to extraction with phosphate-buffered saline. The extract volume ...

  11. Castor oil induces laxation and uterus contraction via ricinoleic acid activating prostaglandin EP3 receptors.

    Science.gov (United States)

    Tunaru, Sorin; Althoff, Till F; Nüsing, Rolf M; Diener, Martin; Offermanns, Stefan

    2012-06-05

    Castor oil is one of the oldest drugs. When given orally, it has a laxative effect and induces labor in pregnant females. The effects of castor oil are mediated by ricinoleic acid, a hydroxylated fatty acid released from castor oil by intestinal lipases. Despite the wide-spread use of castor oil in conventional and folk medicine, the molecular mechanism by which ricinoleic acid acts remains unknown. Here we show that the EP(3) prostanoid receptor is specifically activated by ricinoleic acid and that it mediates the pharmacological effects of castor oil. In mice lacking EP(3) receptors, the laxative effect and the uterus contraction induced via ricinoleic acid are absent. Although a conditional deletion of the EP(3) receptor gene in intestinal epithelial cells did not affect castor oil-induced diarrhea, mice lacking EP(3) receptors only in smooth-muscle cells were unresponsive to this drug. Thus, the castor oil metabolite ricinoleic acid activates intestinal and uterine smooth-muscle cells via EP(3) prostanoid receptors. These findings identify the cellular and molecular mechanism underlying the pharmacological effects of castor oil and indicate a role of the EP(3) receptor as a target to induce laxative effects.

  12. An innovative European integrated project: Castor-CO2 from capture to storage

    NARCIS (Netherlands)

    Thiez, P.L.; Mosditchian, G.; Torp, T.; Feron, P.; Ritsema, I.; Zweigel, P.; Lindeberg, E.

    2005-01-01

    This chapter gives an overview of the CASTOR (CO2, from Capture to Storage) R and D project, funded by the European Union (EU) under the 6th Framework Program. With a partnership involving Industry and Research organizations, CASTOR aims at developing new technologies for post-combustion capture and

  13. Physicochemical and sensory characteristics of milk from goats supplemented with castor or licuri oil.

    Science.gov (United States)

    Pereira, R A G; Oliveira, C J B; Medeiros, A N; Costa, R G; Bomfim, M A D; Queiroga, R C R E

    2010-02-01

    The purpose of this study was to evaluate the influence of castor and licuri palm oils supplemented to milking goats on the physical, chemical, and sensory characteristics of milk. A double Latin square experimental design (5x5) using 10 confined crossbred Moxotó-Alpine goats was performed according to the following treatments: nonsupplemented (control), 3% castor oil, 5% castor oil, 3% licuri oil, and 5% licuri oil. Oils in each treatment were supplemented in the dry matter. Castor oil supplementation reduced the fat content and increased the lactose and density of milk. Considering the sensory analysis, a lower acceptability was observed for milk from goats supplemented with castor oil. On the other hand, licuri oil supplementation led to higher acceptability scores for flavor and odor of goat milk. Copyright 2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  14. Synthesis and membrane performance characterization of self-emulsified waterborne nitrocellulose dispersion modified with castor oil

    Science.gov (United States)

    Su, Xiuxia; Zhao, Qingxiao; Zhang, Dan; Dong, Wei

    2015-11-01

    Waterborne nitrocellulose dispersion modified with castor oil (CWNC) was designed and successfully synthesized by self emulsification and reaction among isophorone diisocyanate (IPDI) trimer, dimethylol propionic acid (DMPA), nitrocellulose (NC) and castor oil (C.O.). The CWNC was characterized by transmission electron microscope (TEM), Fourier transform infrared (FTIR) spectroscopy and thermal gravimetric analysis (TGA), etc. The particle size of CWNC increased with the increase of mass fraction of castor oil to total reactants, ω (C.O.). The morphology of particles is an approximate core-shell structure indicated by TEM. FTIR confirmed that the reactions (i.e. IPDI trimer and castor oil, IPDI trimer and NC) occurred, the NCO groups of IPDI trimer were consumed totally and the backbone of NC was retained. The water contact angle measurements confirmed that introduced castor oil increased hydrophobicity of the film, thereby increasing the contact angle. TGA revealed that the CWNC film had better thermal resistance.

  15. Role of carriers in the transmission of pneumonia in bighorn sheep (Ovis canadensis).

    Science.gov (United States)

    Raghavan, Bindu; Erickson, Kayla; Kugadas, Abirami; Batra, Sai A; Call, Douglas R; Davis, Margaret A; Foreyt, William J; Srikumaran, Subramaniam

    2016-06-15

    In the absence of livestock contact, recurring lamb mortality in bighorn sheep (Ovis canadensis) populations previously exposed to pneumonia indicates the likely presence of carriers of pneumonia-causing pathogens, and possibly inadequate maternally derived immunity. To investigate this problem we commingled naïve, pregnant ewes (n=3) with previously exposed rams (n=2). Post-commingling, all ewes and lambs born to them acquired pneumonia-causing pathogens (leukotoxin-producing Pasteurellaceae and Mycoplasma ovipneumoniae), with subsequent lamb mortality between 4-9 weeks of age. Infected ewes became carriers for two subsequent years and lambs born to them succumbed to pneumonia. In another experiment, we attempted to suppress the carriage of leukotoxin-producing Pasteurellaceae by administering an antibiotic to carrier ewes, and evaluated lamb survival. Lambs born to both treatment and control ewes (n=4 each) acquired pneumonia and died. Antibody titers against leukotoxin-producing Pasteurellaceae in all eight ewes were 'protective' (>1:800 and no apparent respiratory disease); however their lambs were either born with comparatively low titers, or with high (but non-protective) titers that declined rapidly within 2-8 weeks of age, rendering them susceptible to fatal disease. Thus, exposure to pneumonia-causing pathogens from carrier ewes, and inadequate titers of maternally derived protective antibodies, are likely to render bighorn lambs susceptible to fatal pneumonia. © 2016. Published by The Company of Biologists Ltd.

  16. Bilateral uric acid nephrolithiasis and ureteral hypertrophy in a free-ranging river otter (Lontra canadensis)

    Science.gov (United States)

    Grove, Robert A.; Bildfell, Rob; Henny, Charles J.; Buhler, D.R.

    2003-01-01

    We report the first case of uric acid nephrolithiasis in a free-ranging river otter (Lontra canadensis). A 7 yr old male river otter collected from the Skagit River of western Washington (USA) had bilateral nephrolithiasis and severely enlarged ureters (one of 305 examined [0.33%]). The uroliths were 97% uric acid and 3% protein. Microscopic changes in the kidney were confined to expansion of renal calyces, minor loss of medullary tissue, and multifocal atrophy of the cortical tubules. No inflammation was observed in either kidney or the ureters. The ureters were enlarged due to marked hypertrophy of smooth muscle plus dilation of the lumen. Fusion of the major calyces into a single ureteral lumen was several cm distal to that of two adult male otters used as histopathologic control specimens. This case report is part of a large contaminant study of river otters collected from Oregon and Washington. It is important to understand diseases and lesions of the otter as part of our overall evaluation of this population.

  17. Role of carriers in the transmission of pneumonia in bighorn sheep (Ovis canadensis

    Directory of Open Access Journals (Sweden)

    Bindu Raghavan

    2016-06-01

    Full Text Available In the absence of livestock contact, recurring lamb mortality in bighorn sheep (Ovis canadensis populations previously exposed to pneumonia indicates the likely presence of carriers of pneumonia-causing pathogens, and possibly inadequate maternally derived immunity. To investigate this problem we commingled naïve, pregnant ewes (n=3 with previously exposed rams (n=2. Post-commingling, all ewes and lambs born to them acquired pneumonia-causing pathogens (leukotoxin-producing Pasteurellaceae and Mycoplasma ovipneumoniae, with subsequent lamb mortality between 4-9 weeks of age. Infected ewes became carriers for two subsequent years and lambs born to them succumbed to pneumonia. In another experiment, we attempted to suppress the carriage of leukotoxin-producing Pasteurellaceae by administering an antibiotic to carrier ewes, and evaluated lamb survival. Lambs born to both treatment and control ewes (n=4 each acquired pneumonia and died. Antibody titers against leukotoxin-producing Pasteurellaceae in all eight ewes were ‘protective’ (>1:800 and no apparent respiratory disease; however their lambs were either born with comparatively low titers, or with high (but non-protective titers that declined rapidly within 2-8 weeks of age, rendering them susceptible to fatal disease. Thus, exposure to pneumonia-causing pathogens from carrier ewes, and inadequate titers of maternally derived protective antibodies, are likely to render bighorn lambs susceptible to fatal pneumonia.

  18. Characterization and Digestibility of Detoxified Castor Oil Meal for Japanese Quails

    Directory of Open Access Journals (Sweden)

    PA dos Santos

    2015-12-01

    Full Text Available ABSTRACT These experiments were performed to determine the chemical composition, coefficients of nutrient and energy metabolizability, amino acid composition, and cytotoxicity of different castor oil meals subjected to different detoxification processes and added to the diet of Japanese quails. In the trial, 180 46-d-old female Japanese quails were distributed according to a completely randomized design into five treatments and with replicates of six bird each. The treatments consisted of following detoxification methods of castor oil meal: Castor oil meal A (CMA - recovery in alcohol at 80 °C for 20 minutes and drying at 80 °C; castor oil meal B (CMB and C (CMC - recovery in alcohol at 80 °C for 6 minutes, neutralization with 5% NaOH, and drying under direct sunlight sun for two days (CMB or pelleted (CMC; castor oil meal D (CMD - recovery in alcohol at 110 °C for 15 minutes and drying at 110 °C. Castor oil meal was added replacing 20% of the reference diet. There was slight chemical composition variation (1.21% in crude protein, 6% in dry matter, 2.2% in ether extract and 64 kcal/kg in gross energy among the castor oil meals submitted to the different treatments. The castor oil meal submitted to treatment C showed the highest amino acid values. In the cytotoxicity test, treatment D presented lower ricin activity. Castor oil meals A, C, and D may be included in Japanese quail diets; however, castor oil meal D is recommended due to the simplicity its industrial process, its low toxicity, and metabolizability coefficients obtained.

  19. Fusobacterium necrophorum in North American Bighorn Sheep ( Ovis canadensis ) Pneumonia.

    Science.gov (United States)

    Shanthalingam, Sudarvili; Narayanan, Sanjeevkumar; Batra, Sai Arun; Jegarubee, Bavananthasivam; Srikumaran, Subramaniam

    2016-07-01

    Fusobacterium necrophorum has been detected in pneumonic bighorn sheep (BHS; Ovis canadensis ) lungs, in addition to the aerobic respiratory pathogens Mannheimia haemolytica , Bibersteinia trehalosi , Pasteurella multocida , and Mycoplasma ovipneumoniae . Similar to M. haemolytica , F. necrophorum produces a leukotoxin. Leukotoxin-induced lysis and degranulation of polymorphonuclear leukocytes (PMNs) and macrophages are responsible for acute inflammation and lung tissue damage characteristic of M. haemolytica -caused pneumonia. As one approach in elucidating the role of F. necrophorum in BHS pneumonia, we determined the frequency of the presence of F. necrophorum in archived pneumonic BHS lung tissues, and susceptibility of BHS leukocytes to F. necrophorum leukotoxin. A species-specific PCR assay detected F. necrophorum in 37% of pneumonic BHS lung tissues (total tested n=70). Sequences of PCR amplicons were similar to the less virulent F. necrophorum subsp. funduliforme. Fusobacterium necrophorum leukotoxin exhibited cytotoxicity to BHS PMNs and peripheral blood mononuclear cells. As with the M. haemolytica leukotoxin, F. necrophorum leukotoxin was more toxic to BHS PMNs than domestic sheep PMNs. It is likely that F. necrophorum enters the lungs after M. haemolytica and other aerobic respiratory pathogens enter the lungs and initiate tissue damage, thereby creating a microenvironment that is conducive for anaerobic bacterial growth. In summary, Fusobacterium leukotoxin is highly toxic for BHS leukocytes; however, based on the PCR findings, it is unlikely to play a direct role in the development of BHS pneumonia.

  20. 226Ra and other radionuclides in water, vegetation, and tissues of beavers (Castor canadensis) from a watershed containing U tailings neat Elliot Lake, Canada

    International Nuclear Information System (INIS)

    Clulow, F.V.; Mirka, M.A.; Dave, N.K.; Lim, T.P.

    1991-01-01

    Radionuclide levels were measured in tissues, gut contents, diet items, and water at site of capture, of adult beavers from the Serpent River drainage basin which contains U tailings at Elliot Lake, Ontario, and from nearby control sites. Levels of 226 Ra in beaver bone, muscle and kidney were highest in animals from locations close to U tailing; liver levels did not vary by site. Environmental 226 Ra levels were within ranges previously reported at these or similar locations elsewhere; levels in beaver gut contents reflected levels in diet items. Concentration ratios exceeded unity only between some vegetation items and beaver bone at the Elliot Lake site and were less than 0.19 between vegetation and other tissues. In two beavers with tissue levels of 226 Ra higher than others sampled, neither 232 Th nor 230 Th were detected in bone, muscle or liver tissues. U-238 was measurable in bone, muscle and liver; 228 Th in bone, 210 Po bone, muscle and liver; and 210 Pb was measurable only in bone. Estimated yearly intakes of radionuclides by people eating beavers were calculated to be below current allowable levels set by the Canadian regulatory authorities. (author)

  1. Castor oil biodiesel and its blends as alternative fuel

    International Nuclear Information System (INIS)

    Berman, Paula; Nizri, Shahar; Wiesman, Zeev

    2011-01-01

    Intensive production and commercialization of biodiesel from edible-grade sources have raised some critical environmental concerns. In order to mitigate these environmental consequences, alternative oilseeds are being investigated as biodiesel feedstocks. Castor (Ricinus communis L.) is one of the most promising non-edible oil crops, due to its high annual seed production and yield, and since it can be grown on marginal land and in semi-arid climate. Still, few studies are available regarding its fuel-related properties in its pure form or as a blend with petrodiesel, many of which are due to its extremely high content of ricinoleic acid. In this study, the specifications in ASTM D6751 and D7467 which are related to the fatty acid composition of pure castor methyl esters (B100) and its blend with petrodiesel in a 10% vol ratio (B10) were investigated. Kinematic viscosity and distillation temperature of B100 (15.17 mm 2 s -1 and 398.7 o C respectively) were the only two properties which did not meet the appropriate standard limits. In contrast, B10 met all the specifications. Still, ASTM D7467 requires that the pure biodiesel meets the requirements of ASTM D6751. This can limit the use of a wide range of feedstocks, including castor, as alternative fuel, especially due to the fact that in practice vehicles normally use low level blends of biodiesel and petrodiesel. These issues are discussed in depth in the present study. -- Highlights: → CaME can be used as a biodiesel alternative feedstock when blended in petrodiesel. → Due to the high levels of ricinoleic acid maximum blending level is limited to 10%. → Today, CaME blends are not a viable alternative feedstock. → ASTM D7467 requires that pure biodiesel must meet all the appropriate limits.

  2. Isocyanate-functionalized castor oil as a novel bitumen modifier

    OpenAIRE

    Cuadri Vega, Antonio Abad; García Morales, Moisés; Navarro Domínguez, Francisco Javier; Partal López, Pedro

    2013-01-01

    The use of biomaterials from renewable sources in the synthesis of polyurethane-derived polymers is lately receiving great attention from social, environmental and economic standpoints. In this work, prepolymers having different -NCO/-OH ratio were synthesized, by reaction of 4,4´-diphenylmethane diisocyanate (MDI) with castor oil (CO), to be used as modifying agent of asphaltic bitumen. Reactions between MDI and CO, performed with -NCO/-OH molar ratios of 8:1 and 4:1, have led to suitable bi...

  3. Control of plasma position in the CASTOR tokamak

    International Nuclear Information System (INIS)

    Valovic, M.

    1988-11-01

    A simple servo-system designed for plasma position control in the CASTOR tokamak is described. Both radial and vertical plasma displacements were minimized using two servo-loops consisting of detection coils, a conventional electric controller and an amplifier operated as an unipolar voltage-controlled current source. To ensure the optimum conditions in the start-up phase of the discharge, currents in the servo-systems were externally preprogrammed. The prescribed plasma position was maintained with the accuracy of 3 mm. The feedback control improves plasma parameters, e.g. it removes the positional disruption at the end of the tokamak discharge. (J.U.). 4 figs., 3 refs

  4. Particle injection into the Castor tokamak by electric arcs

    International Nuclear Information System (INIS)

    Hildebrandt, D.; Juettner, B.; Pursch, H.; Jakubka, K.; Stoeckel, J.; Zacek, F.

    1989-01-01

    The influence of arcing on the tokamak discharge was investigated in the Castor tokamak. A special calibrated gun which emitted tantalum by artificially ignited electric arcs, was used to study the transport of the injected tantalum ions, neutrals and droplets. The injection of tantalum led to an increase in electron density and to a change of plasma position only if the transported charge was higher than 0.01 C. As the naturally occurring arcs are well below this limit, the arcing in tokamaks is rather the consequence than the reason of instabilities. (J.U.)

  5. Safety analysis report for packaging (onsite) Castor GSF cask

    International Nuclear Information System (INIS)

    Clements, E.P.

    1997-01-01

    The CASTOR GSF packaging was designed and fabricated to be a certified Type B(U) packaging and comply with the requirements of the International Atomic Energy Agency (IAEA) for transport of up to five sealed canisters of vitrified radioactive materials. This onsite Safety Analysis Report for Packaging (SARP) provides the analysis and evaluations necessary to demonstrate that the casks, with the canister payload, meet the intent of the Type B packaging regulations set forth in 10 CFR 71 and therefore meet the onsite transportation safety requirements of WHC-CM-2-14, Hazardous Material Packaging and Shipping

  6. The Echinococcus canadensis (G7) genome: a key knowledge of parasitic platyhelminth human diseases.

    Science.gov (United States)

    Maldonado, Lucas L; Assis, Juliana; Araújo, Flávio M Gomes; Salim, Anna C M; Macchiaroli, Natalia; Cucher, Marcela; Camicia, Federico; Fox, Adolfo; Rosenzvit, Mara; Oliveira, Guilherme; Kamenetzky, Laura

    2017-02-27

    The parasite Echinococcus canadensis (G7) (phylum Platyhelminthes, class Cestoda) is one of the causative agents of echinococcosis. Echinococcosis is a worldwide chronic zoonosis affecting humans as well as domestic and wild mammals, which has been reported as a prioritized neglected disease by the World Health Organisation. No genomic data, comparative genomic analyses or efficient therapeutic and diagnostic tools are available for this severe disease. The information presented in this study will help to understand the peculiar biological characters and to design species-specific control tools. We sequenced, assembled and annotated the 115-Mb genome of E. canadensis (G7). Comparative genomic analyses using whole genome data of three Echinococcus species not only confirmed the status of E. canadensis (G7) as a separate species but also demonstrated a high nucleotide sequences divergence in relation to E. granulosus (G1). The E. canadensis (G7) genome contains 11,449 genes with a core set of 881 orthologs shared among five cestode species. Comparative genomics revealed that there are more single nucleotide polymorphisms (SNPs) between E. canadensis (G7) and E. granulosus (G1) than between E. canadensis (G7) and E. multilocularis. This result was unexpected since E. canadensis (G7) and E. granulosus (G1) were considered to belong to the species complex E. granulosus sensu lato. We described SNPs in known drug targets and metabolism genes in the E. canadensis (G7) genome. Regarding gene regulation, we analysed three particular features: CpG island distribution along the three Echinococcus genomes, DNA methylation system and small RNA pathway. The results suggest the occurrence of yet unknown gene regulation mechanisms in Echinococcus. This is the first work that addresses Echinococcus comparative genomics. The resources presented here will promote the study of mechanisms of parasite development as well as new tools for drug discovery. The availability of a high

  7. Gamma-ray induced mutants in castor (Ricinus communis L.)

    International Nuclear Information System (INIS)

    Janila, P.; Ashok Kumar, A.; Rajashekar Reddy, N.; Hemalatha, V.

    2007-01-01

    We report isolation of three recessive mutants in castor using dry seed irradiation with gamma rays. The crinkled leaf mutant (crf) was identified in K-55-112 M2 family and leafy mutant (lea) in H-55-577 M2 family; both are recessive lethal and thus maintained as heterozygotes. The cri mutant has highly wrinkled leaves resembling finger millet head and failed to enter reproductive phase, consequently did not produce seeds. The number of leaf lobes is reduced in lea mutant and though it produced spikes, the male and female flowers are converted to leafy appendages. The third mutant, fused (Ius) stem identified in H-55-617 M2 family is a recessive mutant. The branches of which are fused at the base and though each branch terminates in to monoceous spike like normal plant, the spike is highly condensed. The three mutants under report are valuable genetic stocks for development of linkage maps in castor, which is at infancy. (author)

  8. Higroscopic properties of castor seeds (Ricinus Comunis L.

    Directory of Open Access Journals (Sweden)

    M. C. Gely

    2009-03-01

    Full Text Available Sorption isotherms of castor seeds were evaluated at 30, 40, 50 and 60ºC using the static gravimetric method. Sigmoid trends were observed for isotherms obtained showing a clear effect of temperature. Experimental values were adjusted using the modified isotherms of Henderson, Halsey, Oswin and Guggenheim-Anderson-de Boer (GAB. Models were compared using four statistics parameters. The results showed that Modified Henderson model adjusted adequately the experimental values in the range of temperature evaluated. The experimental values of the safe storage moisture content for castor seeds varied between 6.1 to 8.4 % (d.b. between 20 and 40ºC, showing lower values than those reported for other traditional oilseeds as sunflower or rapeseed. The sorption heats evaluated from the combination of Clapeyron equation and Modified Henderson equation varied between 3400 and 2373 kJ/kg for moisture contents between 1 and 28 % (d.b.. Through Othmer relationship the value of the ratio Lb/Lf varied between 1.01 and 1.34 (for 14.5 and 1% d.b. moisture content respectively. The values obtained showed an exponential relationship with moisture content, of the form Lb/Lf=1+0.44exp(-0.17M with a determination coefficient R² of 0.97.

  9. Characterization of Novel Castor Oil-Based Polyurethane Polymer Electrolytes

    Directory of Open Access Journals (Sweden)

    Salmiah Ibrahim

    2015-04-01

    Full Text Available Castor oil-based polyurethane as a renewable resource polymer has been synthesized for application as a host in polymer electrolyte for electrochemical devices. The polyurethane was added with LiI and NaI in different wt% to form a film of polymer electrolytes. The films were characterized by using attenuated total reflectance-Fourier transform infrared spectroscopy, dynamic mechanical analysis, electrochemical impedance spectroscopy, linear sweep voltammetry and transference number measurement. The highest conductivity of 1.42 × 10−6 S cm−1 was achieved with the addition of 30 wt% LiI and 4.28 × 10−7 S·cm−1 upon addition of 30 wt% NaI at room temperature. The temperature dependence conductivity plot indicated that both systems obeyed Arrhenius law. The activation energy for the PU-LiI and PU-NaI systems were 0.13 and 0.22 eV. Glass transition temperature of the synthesized polyurethane decreased from −15.8 °C to ~ −26 to −28 °C upon salts addition. These characterizations exhibited the castor oil-based polyurethane polymer electrolytes have potential to be used as alternative membrane for electrochemical devices.

  10. Spreader beam analysis for the CASTOR GSF cask

    International Nuclear Information System (INIS)

    Clements, E.P.

    1997-01-01

    The purpose of this report is to document the results of the 150% rated capacity load test performed by DynCorp Hoisting and Rigging on the CASTOR GSF special cask lifting beams. The two lifting beams were originally rated and tested at 20,000kg (44,000lb) by the cask manufacturer in Germany. The testing performed by DynCorp rated and tested the lifting beams to 30,000 kg (66,000 lb)+0%, -5%, for Hanford Site use. The CASTOR GSF cask, used to transport isotopic Heat Sources (canisters), must be lifted with its own designed lifting beam system (Figures 1, 2, and 3). As designed, the beam material is RSt 37-2 (equivalent to American Society for Testing and Materials[ASTM] A-570), the eye plate is St 52-2 (equivalent to ASTM A-516), and the lifting pin is St 50 (equivalent to ASTM A-515). The beam has two opposing 58 mm (2.3 in.) diameter by 120 mm(4.7 in.) length, high grade steel pins that engage the cask for lifting. The pins have a manual locking mechanism to prevent disengagement from the casks. The static, gross weight (loaded) of the cask 18,640 kg (41,000 lb) on the pins prevents movement of the pins during lifting. This is due to the frictional force of the cask on the pins when lifting begins

  11. Example of industrial valorisation of derivative products of Castor oil

    Directory of Open Access Journals (Sweden)

    Borg Patrick

    2009-07-01

    Full Text Available Known since antiquity, Castor Oil has been first used in medicine. Now, even if it remains present in small quantities as an excipient in many pharmaceutical specialties, it finds a lot of applicationsin cosmetics, industrial applications and chemical industry. Castor Oil specificity comes from its high content of ricinoleic acid (up to 85% that combines a double bond and an hydroxyl function in the heart of a 18 carbons linear chain. This particular structure is the key of an unique chemistry developed by ARKEMA that gives by thermal cracking a wide range of compounds with either 7 or 11 carbon atoms. A whole range of innovative chemistries and end use products are generated from these base reaction products. They are used in every-day life, to improve our comfort and safety but also in very specific applications with very high technical requirements. Synthesized from undecylenic acid, 11-amino-undecanoic acid, 100% based on renewable resources, is the precursor to biobased polymers combining high performance and sustainability: Rilsan®, Rilsan Fine Powder®, Pebax Rnew®.

  12. Microdistribution of 241Am in structures of submerged macrophyte Elodea canadensis growing in the Yenisei River.

    Science.gov (United States)

    Bondareva, L; Vlasova, I; Mogilnaya, O; Bolsunovsky, A; Kalmykov, S

    2010-01-01

    A submerged macrophyte of the Yenisei River, Elodea canadensis, was used to study the microdistribution of the artificial radionuclide (241)Am among different components of the plant. The total amount of (241)Am added to the experimental system was 1850+/-31 Bq/L. The total amount of (241)Am accumulated by the plants was 182 Bq per sample, or 758,333+/-385 Bq/kg dry mass. It has been found that the major portion of (241)Am accumulated by E. canadensis, up to 85%, was bound to solid components of the cells. It is observed that the microdistribution of (241)Am within different components of the submerged plant E. canadensis was not uniform. (241)Am distribution vary depending on the age of the leaf blades, the state of the cells and morphological features of the plant stem.

  13. Effect of copper on growth of an aquatic macrophyte, Elodea canadensis.

    Science.gov (United States)

    Mal, Tarun K; Adorjan, Peter; Corbett, Andrea L

    2002-01-01

    Elodea canadensis has been proposed as a potential biomonitor due to its wide distribution and apparent ability to accumulate pollutants in aquatic ecosystems. We investigated the effects of copper sulfate on growth in E. canadensis to determine its effectiveness as a biomonitor of copper pollution in aquatic systems and whether growth is a suitable index of sub-lethal stress. Copper sulfate significantly slowed or stopped growth at all concentrations (low: 1 ppm, medium: 5 ppm, high: 10 ppm of copper sulfate) used. Final plant drymass was significantly lower in medium and high copper treatments compared with controls. E. canadensis appears to be very sensitive to copper levels, and may be useful as a biomonitor of copper levels in aquatic systems. However, its utility as a bioaccumulator may be limited, because we observed senescence of most leaves in all copper-treated plants following 4 weeks of treatment.

  14. Adsorption, Bioaccumulation and Kinetics Parameters of the Phytoremediation of Cobalt from Wastewater Using Elodea canadensis.

    Science.gov (United States)

    Mosoarca, Giannin; Vancea, Cosmin; Popa, Simona; Boran, Sorina

    2018-05-01

    Present paper investigates the phytoremediation of cobalt from wastewaters using Elodea canadensis. Bioaccumulation tests were conducted at various concentrations of cobalt ranging from 1 to 15 mg/L. Final concentrations of cobalt in wastewaters, after phytoremediation, were less than 1 mg/L. E. canadensis' hyperaccumulator character with regard to cobalt is emphasised by the amount of cobalt retained: 0.39% ± 0.02% of dry mass at an initial concentration in wastewater of 15 mg/L. After 14 days of exposure to contaminant, the biomass as well as the relative growth rate has increased with the amount of cobalt in wastewaters, the plant manifesting an excellent tolerance to cobalt exposure. Adsorption of cobalt ions by E. canadensis can be well described by the Langmuir adsorption isotherm and the pseudo-second-order model equation.

  15. Microdistribution of 241Am in structures of submerged macrophyte Elodea canadensis growing in the Yenisei River

    International Nuclear Information System (INIS)

    Bondareva, L.; Vlasova, I.; Mogilnaya, O.; Bolsunovsky, A.; Kalmykov, S.

    2010-01-01

    A submerged macrophyte of the Yenisei River, Elodea canadensis, was used to study the microdistribution of the artificial radionuclide 241 Am among different components of the plant. The total amount of 241 Am added to the experimental system was 1850 ± 31 Bq/L. The total amount of 241 Am accumulated by the plants was 182 Bq per sample, or 758,333 ± 385 Bq/kg dry mass. It has been found that the major portion of 241 Am accumulated by E. canadensis, up to 85%, was bound to solid components of the cells. It is observed that the microdistribution of 241 Am within different components of the submerged plant E. canadensis was not uniform. 241 Am distribution vary depending on the age of the leaf blades, the state of the cells and morphological features of the plant stem.

  16. Costs and benefits of group living with disease: a case study of pneumonia in bighorn lambs (Ovis canadensis).

    Science.gov (United States)

    Manlove, Kezia R; Cassirer, E Frances; Cross, Paul C; Plowright, Raina K; Hudson, Peter J

    2014-12-22

    Group living facilitates pathogen transmission among social hosts, yet temporally stable host social organizations can actually limit transmission of some pathogens. When there are few between-subpopulation contacts for the duration of a disease event, transmission becomes localized to subpopulations. The number of per capita infectious contacts approaches the subpopulation size as pathogen infectiousness increases. Here, we illustrate that this is the case during epidemics of highly infectious pneumonia in bighorn lambs (Ovis canadensis). We classified individually marked bighorn ewes into disjoint seasonal subpopulations, and decomposed the variance in lamb survival to weaning into components associated with individual ewes, subpopulations, populations and years. During epidemics, lamb survival varied substantially more between ewe-subpopulations than across populations or years, suggesting localized pathogen transmission. This pattern of lamb survival was not observed during years when disease was absent. Additionally, group sizes in ewe-subpopulations were independent of population size, but the number of ewe-subpopulations increased with population size. Consequently, although one might reasonably assume that force of infection for this highly communicable disease scales with population size, in fact, host social behaviour modulates transmission such that disease is frequency-dependent within populations, and some groups remain protected during epidemic events. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

  17. Costs and benefits of group living with disease: a case study of pneumonia in bighorn lambs (Ovis canadensis)

    Science.gov (United States)

    Manlove, Kezia R.; Cassirer, E. Frances; Cross, Paul C.; Plowright, Raina K.; Hudson, Peter J.

    2014-01-01

    Group living facilitates pathogen transmission among social hosts, yet temporally stable host social organizations can actually limit transmission of some pathogens. When there are few between-subpopulation contacts for the duration of a disease event, transmission becomes localized to subpopulations. The number of per capita infectious contacts approaches the subpopulation size as pathogen infectiousness increases. Here, we illustrate that this is the case during epidemics of highly infectious pneumonia in bighorn lambs (Ovis canadensis). We classified individually marked bighorn ewes into disjoint seasonal subpopulations, and decomposed the variance in lamb survival to weaning into components associated with individual ewes, subpopulations, populations and years. During epidemics, lamb survival varied substantially more between ewe-subpopulations than across populations or years, suggesting localized pathogen transmission. This pattern of lamb survival was not observed during years when disease was absent. Additionally, group sizes in ewe-subpopulations were independent of population size, but the number of ewe-subpopulations increased with population size. Consequently, although one might reasonably assume that force of infection for this highly communicable disease scales with population size, in fact, host social behaviour modulates transmission such that disease is frequency-dependent within populations, and some groups remain protected during epidemic events.

  18. Enzymatic basis for fungicide removal by Elodea canadensis.

    Science.gov (United States)

    Dosnon-Olette, Rachel; Schröder, Peter; Bartha, Bernadett; Aziz, Aziz; Couderchet, Michel; Eullaffroy, Philippe

    2011-07-01

    Plants can absorb a diversity of natural and man-made toxic compounds for which they have developed diverse detoxification mechanisms. Plants are able to metabolize and detoxify a wide array of xenobiotics by oxidation, sugar conjugation, glutathione conjugation, and more complex reactions. In this study, detoxification mechanisms of dimethomorph, a fungicide currently found in aquatic media were investigated in Elodea canadensis. Cytochrome P450 (P450) activity was measured by an oxygen biosensor system, glucosyltransferases (GTs) by HPLC, glutathione S-transferases (GSTs), and ascorbate peroxidase (APOX) were assayed spectrophotometrically. Incubation of Elodea with dimethomorph induced an increase of the P450 activity. GST activity was not stimulated by dimethomorph suggesting that GST does not participate in dimethomorph detoxification. In plants exposed to dimethomorph, comparable responses were observed for GST and APOX activities showing that the GST was more likely to play a role in response to oxidative stress. Preincubation with dimethomorph induced a high activity of O- and N-GT, it is therefore likely that both enzymes participate in the phase II (conjugation) of dimethomorph detoxification process. For the first time in aquatic plants, P450 activity was shown to be induced by a fungicide suggesting a role in the metabolization of dimethomorph. Moreover, our finding is the first evidence of dimethomorph and isoproturon activation of cytochrome P450 multienzyme family in an aquatic plant, i.e., Elodea (isoproturon was taken here as a reference molecule). The detoxification of dimetomorph seems to proceed via hydroxylation, and subsequent glucosylation, and might yield soluble as well as cell wall bound residues.

  19. Seed borne mycoflora of castor bean (ricinus communis l.) from pakistan

    International Nuclear Information System (INIS)

    Dawar, S.; Khalid, S.

    2014-01-01

    Castor bean seeds were analyzed by using ISTA (International seed testing association) for the detection of seed borne mycoflora. Thirty one fungal species belonging to 15 genera were isolated from 12 samples of castor bean seeds collected from different areas of Pakistan. Fusarium solani, Alternaria alternata, Cephaliophora tropica were most predominant fungal species isolated while the saprophytic fungi like A. niger, A. flavus were common in all samples of castor bean seed tested. Blotter method was considered to be better technique which gave maximum number of fungi followed by agar plate and deep freezing methods. (author)

  20. Arbuscular mycorrhizal fungi facilitate the invasion of Solidago canadensis L. in southeastern China

    Science.gov (United States)

    Yang, Ruyi; Zhou, Gang; Zan, Shuting; Guo, Fuyu; Su, Nannan; Li, Jing

    2014-11-01

    The significance of arbuscular mycorrhizal fungi (AMF) in the process of plant invasion is still poorly understood. We hypothesize that invasive plants would change local AMF community structure in a way that would benefit themselves but confer less advantages to native plants, thus influencing the extent of plant interactions. An AMF spore community composed of five morphospecies of Glomus with equal density (initial AMF spore community, I-AMF) was constructed to test this hypothesis. The results showed that the invasive species, Solidago canadensis, significantly increased the relative abundance of G. geosperum and G. etunicatum (altered AMF spore community, A-AMF) compared to G. mosseae, which was a dominant morphospecies in the monoculture of native Kummerowia striata. The shift in AMF spore community composition driven by S. canadensis generated functional variation between I-AMF and A-AMF communities. For example, I-AMF increased biomass and nutrient uptake of K. striata in both monocultures and mixtures of K. striata and S. canadensis compared to A-AMF. In contrast, A-AMF significantly enhanced root nitrogen (N) acquisition of S. canadensis grown in mixture. Moreover, mycorrhizal-mediated 15N uptake provided direct evidence that I-AMF and A-AMF differed in their affinities with native and invading species. The non-significant effect of A-AMF on K. striata did not result from allelopathy as root exudates of S. canadensis exhibited positive effects on seed germination and biomass of K. striata under naturally occurring concentrations. When considered together, we found that A-AMF facilitated the invasion of S. canadensis through decreasing competitiveness of the native plant K. striata. The results supported our hypothesis and can be used to improve our understanding of an ecosystem-based perspective towards exotic plant invasion.

  1. Haematology and Serum Biochemistry Parameters and Variations in the Eurasian Beaver (Castor fiber).

    Science.gov (United States)

    Girling, Simon J; Campbell-Palmer, Roisin; Pizzi, Romain; Fraser, Mary A; Cracknell, Jonathan; Arnemo, Jon; Rosell, Frank

    2015-01-01

    Haematology parameters (N = 24) and serum biochemistry parameters (N = 35) were determined for wild Eurasian beavers (Castor fiber), between 6 months - 12 years old. Of the population tested in this study, N = 18 Eurasian beavers were from Norway and N = 17 originating from Bavaria but now living extensively in a reserve in England. All blood samples were collected from beavers via the ventral tail vein. All beavers were chemically restrained using inhalant isoflurane in 100% oxygen prior to blood sampling. Results were determined for haematological and serum biochemical parameters for the species and were compared between the two different populations with differences in means estimated and significant differences being noted. Standard blood parameters for the Eurasian beaver were determined and their ranges characterised using percentiles. Whilst the majority of blood parameters between the two populations showed no significant variation, haemoglobin, packed cell volume, mean cell haemoglobin and white blood cell counts showed significantly greater values (pbeavers or between sexually immature (beavers in the animals sampled. With Eurasian beaver reintroduction encouraged by legislation throughout Europe, knowledge of baseline blood values for the species and any variations therein is essential when assessing their health and welfare and the success or failure of any reintroduction program. This is the first study to produce base-line blood values and their variations for the Eurasian beaver.

  2. Unbodied dehydrated castor oil from oil of castor beans cultivated in tropical and sub tropical zones of pakistan

    International Nuclear Information System (INIS)

    Saleem, R.; Sultana, R.; Ambrat, A.; Kandhro, A.

    2014-01-01

    Commercially available castor oil was analysed for specific gravity at 25/25 degree C (0.959), acid value (0.5), iodine value (85), refractive index at 25 degree C (1.4769), viscosity at 25 degree C (6.8), and hydroxyl value (162). Fatty acid composition, including palmitic acid (I. 7%), stearic acid (1.39%), oleic acid (5.73%), linoleic acid (6.25%), and ricinoleic acid (84.2%) was estimated by means of GLC. Unbodied dehydrated castor oil (DCO- 15) 93% was prepared by using sodium bisulphate and sodium bisulphite and other reaction conditions. Unbodied DCO was analysed for iodine value (139), acid value (3.6), refractive index at 25 degree C (1.4820), specific gravity at 25/25 degree C and 30/30 degree C (0.9333 and 0.925) viscosity at 25 degree C and 30 degree C (200 cps and 1.85 poise), respectively. Fatty acid composition was also determined to ascertain the extent of dehydration due to the formation of conjugated linoleic acid (CLA, 36.9%) and non conjugated linoleic acid (NCLA, 46.8%). Ricinoleic acid was estimated as 3.83%. Unbodied DCO-15 was in good conformance with the standard specification of ASTM and other values cited in the literature. (author)

  3. Enzymatic incorporation of caffeoyl into castor oil to prepare the novel castor oil-based caffeoyl structured lipids.

    Science.gov (United States)

    Sun, Shangde; Wang, Ping; Zhu, Sha

    2017-05-10

    In this work, a novel castor oil-based caffeoyl structured lipids was successfully prepared by the enzymatic transesterification using castor oil (CO) as caffeoyl acceptor. During the structured lipids preparation, two competitive reactions, the hydrolysis of CO to form hydrophilic caffeoyl glycerols (CG)+dicaffeoyl glycerols (DCG) and the transesterification of CO with ethyl caffeate (EC) to form lipophilic caffeoyl mono- and di-acylglycerols (CMAG and CDAG), were found. Reaction progress was monitored using HPLC-ESI-MS and HPLC-UV. The effects of by-product ethanol removal and reaction variables on the transesterification and reaction selectivity were evaluated. Results showed that, the activation energies for the transesterification and for the selective formations of CMAG+CDAG and CG+DCG were 57.60kJ/mol, 58.86kJ/mol, and 60.53kJ/mol, respectively. Under the optimal reaction conditions (enzyme load 23%, 90°C, 1:3 molar ratio of EC to CO, and 46.5h), EC conversion and the yield of CMAG+CDAG were 93.68±2.52% and 78.11±1.35%, respectively. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Ancient mitochondrial DNA and the genetic history of Eurasian beaver (Castor fiber) in Europe.

    Science.gov (United States)

    Horn, Susanne; Prost, Stefan; Stiller, Mathias; Makowiecki, Daniel; Kuznetsova, Tatiana; Benecke, Norbert; Pucher, Erich; Hufthammer, Anne K; Schouwenburg, Charles; Shapiro, Beth; Hofreiter, Michael

    2014-04-01

    After centuries of human hunting, the Eurasian beaver Castor fiber had disappeared from most of its original range by the end of the 19th century. The surviving relict populations are characterized by both low genetic diversity and strong phylogeographical structure. However, it remains unclear whether these attributes are the result of a human-induced, late Holocene bottleneck or already existed prior to this reduction in range. To investigate genetic diversity in Eurasian beaver populations during the Holocene, we obtained mitochondrial control region DNA sequences from 48 ancient beaver samples and added 152 modern sequences from GenBank. Phylogeographical analyses of the data indicate a differentiation of European beaver populations into three mitochondrial clades. The two main clades occur in western and eastern Europe, respectively, with an early Holocene contact zone in eastern Europe near a present-day contact zone. A divergent and previously unknown clade of beavers from the Danube Basin survived until at least 6000 years ago, but went extinct during the transition to modern times. Finally, we identify a recent decline in effective population size of Eurasian beavers, with a stronger bottleneck signal in the western than in the eastern clade. Our results suggest that the low genetic diversity and the strong phylogeographical structure in recent beavers are artefacts of human hunting-associated population reductions. While beaver populations have been growing rapidly since the late 19th century, genetic diversity within modern beaver populations remains considerably reduced compared to what was present prior to the period of human hunting and habitat reduction.

  5. Effect of powdered castor oil seed ( Ricinus communis L.) on some ...

    African Journals Online (AJOL)

    Effect of powdered castor oil seed ( Ricinus communis L.) on some internal organs of ... distribution tables, mean, mode and percentages were used for the analysis. ... development agencies and provision of training by relevant stakeholders to ...

  6. Biodiesel Production from Castor Oil and Its Application in Diesel Engine

    Directory of Open Access Journals (Sweden)

    S Ismail

    2014-12-01

    Full Text Available In this study, the optimum biodiesel conversion from crude castor oil to castor biodiesel (CB through transesterification method was investigated. The base catalyzed transesterification under different reactant proportion such as the molar ratio of alcohol to oil and mass ratio of catalyst to oil was studied for optimum production of castor biodiesel. The optimum condition for base catalyzed transesterification of castor oil was determined to be 1:4.5 of oil to methanol ratio and 0.005:1 of potassium hydroxide to oil ratio. The fuel properties of the produced CB such as the calorific value, flash point and density were analyzed and compared to conventional diesel. Diesel engine performance and emission test on different CB blends proved that CB was suitable to be used as diesel blends. CB was also proved to have lower emission compared to conventional diesel.

  7. Comparative clinical trial of castor oil and diclofenac sodium in patients with osteoarthritis.

    Science.gov (United States)

    Medhi, B; Kishore, K; Singh, U; Seth, S D

    2009-10-01

    A randomized, double-blind, comparative clinical study was conducted to compare the safety and efficacy of castor oil with diclofenac sodium in patients with knee osteoarthritis. Subjects with symptoms of knee osteoarthritis were given a castor oil capsule 0.9 mL (n = 50) thrice daily for 4 weeks or a capsule of diclofenac sodium (n = 50), 50 mg thrice daily for 4 weeks. The subjects completed an overall evaluation of symptom relief at 2 weeks and 4 weeks of completed treatment. The subjects were evaluated by clinical, routine laboratory and radiographic investigations for improvement of disease conditions and also for adverse drug reaction. On completion of 4 weeks treatment it was observed that both drugs were significantly effective in the treatment of knee osteoarthritis (p castor oil there were no adverse effects reported. The present study indicates that castor oil can be used as an effective therapy in primary knee osteoarthritis. (c) 2009 John Wiley & Sons, Ltd.

  8. Physiological acclimation to drought stress in Solidago canadensis.

    Science.gov (United States)

    Nolf, Markus; Pagitz, Konrad; Mayr, Stefan

    2014-04-01

    Solidago canadensis is an invasive species from North America that is spreading across Europe, Australia and temperate Asia. We hypothesized that the species' wide ecological amplitude is also based on its potential in hydraulic acclimation, and analyzed hydraulic and anatomical properties along a transect with decreasing soil humidity. Stem hydraulic conductivity, vulnerability to drought-induced embolism, stomatal closure during dehydration and xylem-anatomical parameters were quantified at three sites. At the humid site, specific hydraulic conductivity of stems (1.0 ± 0.2 kg m(-1)  MPa(-1)  s(-1)) was about twofold higher, and leaf-specific conductivity about 1.5 times higher (3.1 ± 0.5 kg m(-1)  MPa(-1)  s(-1)) than at the dry site. Water potential (Ψ) at 50% loss of conductivity was -3.7 ± 0.1 MPa at the dry site and -3.1 ± 0.2 MPa at the humid site (September). Vulnerability to drought-induced embolism decreased along the transect and over the vegetation period. At drier sites, stomata started closing at lower Ψ while complete stomatal closure was reached at less negative Ψ (12% of maximum stomatal conductance: -2.5 ± 0.0 and -3.0 ± 0.2 MPa at the dry and humid site). The safety margin between stomatal closure and 50% loss of conductivity was 1.2 and 0.2 MPa at the dry and humid sites. The observed variability indicated an efficient acclimation in hydraulic conductivity and safety: plants at dry sites exhibited lower specific hydraulic conductivity, higher embolism resistance and broader safety margins, signifying a trade-off between the hydraulic safety and efficiency. The observed intraspecific plasticity in hydraulic and anatomical traits may help to explain the invasive potential of this species. © 2013 Scandinavian Plant Physiology Society.

  9. Molecular techniques to distinguish morphologically similar Hydrilla verticillata, Egeria densa, Elodea nuttallii, and Elodea canadensis

    Science.gov (United States)

    Rybicki, Nancy B.; Kirshtein, Julie D.; Voytek, Mary A.

    2013-01-01

    The four submerged aquatic species, hydrilla (Hydrilla verticillata [monoecious and dioecious]), Brazilian waterweed (Egeria densa), Canadian waterweed (Elodea canadensis), and western waterweed (Elodea nuttallii), are difficult to positively identify because of their morphological similarity to each other, resulting in possible misidentification. This limits our ability to understand their past and present distribution, which is important in aquatic plant management. We investigated a molecular technique to identify these species, which are problematic because of their invasive nature on multiple continents. Approximately 100 samples of these species, ranging in age from 40-yr-old herbarium samples to recently collected plants, were collected from regions across the United States. The distribution and range of the samples collected in this research were compared to those reported in the literature. We confirmed information on the current wide distribution of both hydrilla biotypes in the United States and discovered that hydrilla had actually invaded the waterways near Washington, DC 6 yr earlier than originally reported. In addition, we found evidence of the confusion, dating back to the 1980s, between Canadian waterweed and western waterweed in the mid-Atlantic region of the United States. Canadian waterweed was previously reported as common and western waterweed as rare; however, our samples indicate the opposite is true. This information indicates there is a need for investigators to anticipate the spread of hydrilla populations to northern U.S. waterways, where it will compete with existing plant species, including Canadian and western waterweeds. Our ability to confirm distribution and pace of spread of invasive and noninvasive species will improve with increased application of molecular techniques.

  10. Mechanical and thermal properties of castor oil polyurethane bone cement after gamma irradiation

    International Nuclear Information System (INIS)

    Azevedo, E.C.; Chierice, G.O.; Claro Neto, S.; Lepiesnki, C.M.; Nascimento, E.M.

    2009-01-01

    Polyurethanes from castor oil are being employed as bone cement in medical applications. In this work the thermal and mechanical properties of gamma irradiated polyurethanes derivative from castor oil were investigated by instrumented indentation, thermogravimetry and scanning electron microscopy. A slightly increase in hardness is observed only for doses as high as 100 kGy. Thermal analysis indicates stability at human body temperature. The glass transition temperature has small changes after gamma irradiation. (author)

  11. EVALUATION OF THE LIPOLYTIC ACTIVITY OF FUNGUS ON CASTOR OIL (Ricinuscommunis)

    OpenAIRE

    Mendoza C., L.; Lino N., M.; León Q., J.; Santiago C., J.

    2014-01-01

    The present study aims to select fungal strains having improved lipolytic activity in castor oil and see if its crude enzyme obtained by fermentation in liquid substrate (FSL) has the same activity on castor oil. Nine strains were isolated from fungi, Penicillium sp eight and one Aspergillus sp from waste vegetable oils. The results show that all studied strains of degradation halos developed, however, in no case was the halos of crude enzymatic degradation. The work showed that the strains o...

  12. Dynamics Analysis of Castor Hulling in the Process of Air-and-Screen Cleaning

    Directory of Open Access Journals (Sweden)

    Gao Ruitao

    2016-01-01

    Full Text Available The air-and-screen cleaning mechanism of castor hulling is analyzed in this paper. And the numeric expression equations of the castor dynamics analysis are established. The correlation of floating speeds vp, air speeds w, the direction angle of airflow velocity β, the direction angle of vibration δ, the friction angle ϕ, the slip coefficient, the fell coefficient and the jumping coefficient are analyzed.

  13. Dynamics Analysis of Castor Hulling in the Process of Air-and-Screen Cleaning

    OpenAIRE

    Gao Ruitao; Cao Yuhua; Yao Liangliang; Jin Hong

    2016-01-01

    The air-and-screen cleaning mechanism of castor hulling is analyzed in this paper. And the numeric expression equations of the castor dynamics analysis are established. The correlation of floating speeds vp, air speeds w, the direction angle of airflow velocity β, the direction angle of vibration δ, the friction angle ϕ, the slip coefficient, the fell coefficient and the jumping coefficient are analyzed.

  14. Some properties of castor oil affecting its performance as a capacitor impregnant and their significance to future impregnant research

    International Nuclear Information System (INIS)

    Boicourt, G.P.

    1975-01-01

    For a considerable time castor oil and polychlorinated biphenyl (PCB) have been the principal impregnants used in energy-storage capacitors. Castor oil has proven to be better than PCB for pulsed applications. PCB's have come under attack as an environmental hazard, while castor oil is a vegetable product and its supply and quality are subject to fluctuation. These two facts make the development of new impregnants desirable. The properties of PCB as a capacitor impregnant are well known. This paper first compares a number of properties of castor oil and PCB's. A comparison is made between the lives of castor oil capacitors and comparable PCB energy-storage capacitors. Some of the physical and chemical properties of castor oil which make it a good pulse capacitor impregnant are examined. These properties can be used as a guide for future research on new pulse capacitor impregnants

  15. Castor and Pollux - shielded cells for studying fuel treatment processes

    International Nuclear Information System (INIS)

    Faudot, G.; Bathellier, A.

    1969-01-01

    CASTOR and POLLUX, two alpha, beta, gamma cells are described in the present paper. They are located in the CEN at Fontenay-aux-Roses (France). They are designed for improvement studies of the various aqueous separation processes used in irradiated fuels reprocessing plants. Located in the same air-tight steel encasement, they arc inter-connected by a pneumatic transfer. These two cells have a similar in-line conception and they include: a gamma shielding in lead of 10 cm of thickness; an inner air-tight box, made with stainless steel and plexiglas, is maintained in lowering in comparison to room pressure. Eleven Hobson model seven master-slave manipulators allow inner manipulations. Then the inner equipment is described briefly. (author) [fr

  16. An ohmic heating circuit for the CASTOR tokamak

    International Nuclear Information System (INIS)

    Valovic, M.

    1989-07-01

    To extend the duration of the CASTOR tokamak discharge to the limit given by the toroidal magnetic field pulse, a simple ohmic heating circuit is proposed. It exploits two condenser banks charged to different voltages and switched by means of an ignitron switch. The circuit parameters are chosen so as to achieve optimum current ramp-up and flat-top phases. The choice of parameters was checked using a simple computer code, with the nonlinear magnetization of the transformer core being taken into account. The results of calculation are compared with those of an experimental test shot with a discharge current and duration of 19 kA and 40 ms, respectively. (J.U.). 3 figs., 4 refs

  17. LDPE/PHB blends filled with castor oil cake

    Science.gov (United States)

    Burlein, Gustavo A.; Rocha, Marisa C. G.

    2015-05-01

    The response surface methodology (RSM) is a collection of mathematical techniques useful for developing, improving and optimizing process. In this study, RSM technique was applied to evaluate the effect of the components proportion on the mechanical properties of low density polyethylene (LDPE)/ poly (3-hydroxy-butyrate) (PHB) blends filled with castor oil cake (CC). The blends were prepared by melt mixing in a twin screw extruder. Low density polyethylene, poly (3-hydroxy-butyrate) and castor oil pressed cake were represented by the input variables designated as LDPE, PHB and CC, respectively. As it was desirable to consider the largest LDPE content in the ternary system, the components of the mixture were subjected to the following constraints: 0.7 ≤ LDPE ≤ 1.0, 0≤ PHB≤0.3 e 0 ≤ CC ≤0.3. The mechanical properties of the different mixtures were determined by conventional ASTM tests and were evaluated through analysis of variance performed by the Minitab software. Some polynomial equations were tested in order to describe the mechanical behavior of the samples. The quadratic model in pseudo components was selected for describing the tensile behavior because it was the most efficient from a statistical point of view (p-value ≤ 0.05; coefficient of determination (r2) close to 1 and variation inflation factor (VIF) values PHB or CC. The tensile strength values of binary mixtures of LDPE lie in the range from 8.9 to 10 MPa. As some commercial grades of LDPE have mechanical strength in this range, it may be inferred that the addition of a certain amount of PHB or CC to LDPE may be considered as a possibility for obtaining LDPE based materials with increased susceptibility to biodegradation. The cubic model in pseudo components was selected for describe the flexural strength of the samples because it was the most adequate from a statistical point of view. However, the linear model in pseudo components was the most efficient to describe the flexural modulus

  18. Ingestive behavior of lambs fed diets containing castor seed meal.

    Science.gov (United States)

    Nicory, Isis Miranda Carvalho; de Carvalho, Gleidson Giordano Pinto; Ribeiro, Ossival Lolato; Silva, Robério Rodrigues; Tosto, Manuela Silva Libanio; Costa-Lopes, Lívia Santos; Souza, Fábio Nicory Costa; de Oliveira Nascimento, Camila

    2015-06-01

    The objective of this study was to evaluate the substitution of soybean meal for castor seed meal (CSM) in diets for feedlot lambs and the effects of these diets on their ingestive behavior. Fifty male Santa Inês lambs were used. The diets were composed of Tifton 85 hay and a concentrate containing detoxified CSM substituting 0, 25, 50, 75, or 100 % of the soybean meal. There was no effect (P > 0.05) of the CSM levels on the feeding, rumination, idle times, chews and time spent chewing per bolus, total chewing time, number of boli chewed, and number of chews per day. The dry matter (DM) intake decreased linearly (P  0.05) the numbers of feeding, rumination, and idle periods, but had a quadratic effect (P ingestive behavior.

  19. Castor oil polyurethane containing silica nanoparticles as filling material of bone defect in rats.

    Science.gov (United States)

    Nacer, Renato Silva; Poppi, Rodrigo Ré; Carvalho, Paulo de Tarso Camilo de; Silva, Baldomero Antonio Kato da; Odashiro, Alexandre Nakao; Silva, Iandara Schettert; Delben, José Renato Jurkevicz; Delben, Angela Antonia Sanches Tardivo

    2012-01-01

    To evaluate the biologic behavior of the castor polymer containing silica nanoparticles as a bone substitute in diafisary defect. Twenty seven male Rattus norvegicus albinus Wistar lineage were submitted to bone defect filled with castor oil polymer. Three experimental groups had been formed with nine animals each: (1) castor oil polymer containing only calcium carbonate; (2) castor oil polymer with calcium carbonate and doped with 5% of silica nanoparticles; (3) castor polymer with calcium carbonate doped with 10% of silica nanoparticles; 3 animals of each group were submitted to euthanasia 15, 30 and 60 days after experimental procedure, and their femurs were removed to histological evaluation. there was bone growth in all the studied groups, with a greater tendency of growth in the group 1. After 30 days all the groups presented similar results. After 60 days a greater amount of fibroblasts, osteoblasts, osteocytes and osteoclasts in group 3 was observed, with integrated activity of 3 kinds of cells involved in the bone activation-reabsorption-formation. The castor polymer associated to the silica nanoparticles is biocompatible and allows osteoconduction. The presence of osteoprogenitors cells suggests silica osteoinduction capacity.

  20. Effects of metal lead on growth and mycorrhizae of an invasive plant species (Solidago canadensis L.).

    Science.gov (United States)

    Yang, Ruyi; Yu, Guodong; Tang, Jianjun; Chen, Xin

    2008-01-01

    It is less known whether and how soil metal lead (Pb) impacts the invasion of exotic plants. A greenhouse experiment was conducted to estimate the effects of lead on the growth and mycorrhizae of an invasive species (Solidago canadensis L.) in a microcosm system. Each microcosm unit was separated into HOST and TEST compartments by a replaceable mesh screen that allowed arbuscular mycorrhizal (AM) fungal hyphae rather than plant roots to grow into the TEST compartments. Three Pb levels (control, 300, and 600 mg/kg soil) were used in this study to simulate ambient soil and two pollution sites where S. canadensis grows. Mycorrhizal inoculum comprised five indigenous arbuscular mycorrhizal fungal species (Glomus mosseae, Glomus versiform, Glomus diaphanum, Glomus geosporum, and Glomus etunicatum). The 15N isotope tracer was used to quantify the mycorrhizally mediated nitrogen acquisition of plants. The results showed that S. canadensis was highly dependent on mycorrhizae. The Pb additions significantly decreased biomass and arbuscular mycorrhizal colonization (root length colonized, RLC%) but did not affect spore numbers, N (including total N and 15N) and P uptake. The facilitating efficiency of mycorrhizae on nutrient acquisition was promoted by Pb treatments. The Pb was mostly sequestered in belowground of plant (root and rhizome). The results suggest that the high efficiency of mycorrhizae on nutrient uptake might give S. canadensis a great advantage over native species in Pb polluted soils.

  1. Allelopathic effects of the extracts from an invasive species Solidago canadensis L. on Microcystis aeruginosa.

    Science.gov (United States)

    Huang, Y; Bai, Y; Wang, Y; Kong, H

    2013-11-01

    This study investigated allelopathic effects of Solidago canadensis L. on Microcystis aeruginosa. The results showed that S. canadensis L. extracts could significantly inhibit the growth of M. aeruginosa. The inhibition ratios of samples with 0·3 and 0·5 g l(-1) extracts were over 90% after 7 days, and the transmission electron microscopy images showed the damage of M. aeruginosa cells during the incubation. In physiological and biochemical measurements, the membrane permeability and malondialdehyde (MDA) content rapidly increased with the accumulation of reactive oxygen species (ROS), and the content of antioxidant molecules (ascorbic acid (AsA) and glutathione (GSH)) increased. Although the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT)) increased at low extracts concentrations, the effects were inhibitory when the extracts concentration increased. In conclusion, this study provided a new idea to utilize the detrimental weed S. canadensis L. to control harmful cyanobacteria. The alterations in physiology and biochemistry of M. aeruginosa cell were not in isolation, but with the stimulation of intracellular ROS that could play a fundamental role in inhibitory effects of S. canadensis L. extracts. It was inferred that terrestrial plants could have the same algistatic mechanisms as hydrophytes. © 2013 The Society for Applied Microbiology.

  2. What determines positive, neutral, and negative impacts of Solidago canadensis invasion on native plant species richness?

    Science.gov (United States)

    Dong, Li-Jia; Yu, Hong-Wei; He, Wei-Ming

    2015-11-17

    Whether plant invasions pose a great threat to native plant diversity is still hotly debated due to conflicting findings. More importantly, we know little about the mechanisms of invasion impacts on native plant richness. We examined how Solidago canadensis invasion influenced native plants using data from 291 pairs of invaded and uninvaded plots covering an entire invaded range, and quantified the relative contributions of climate, recipient communities, and S. canadensis to invasion impacts. There were three types of invasion consequences for native plant species richness (i.e., positive, neutral, and negative impacts). Overall, the relative contributions of recipient communities, S. canadensis and climate to invasion impacts were 71.39%, 21.46% and 7.15%, respectively; furthermore, the roles of recipient communities, S. canadensis and climate were largely ascribed to plant diversity, density and cover, and precipitation. In terms of direct effects, invasion impacts were negatively linked to temperature and native plant communities, and positively to precipitation and soil microbes. Soil microbes were crucial in the network of indirect effects on invasion impacts. These findings suggest that the characteristics of recipient communities are the most important determinants of invasion impacts and that invasion impacts may be a continuum across an entire invaded range.

  3. Copper and zinc in Elodea canadensis from rivers with various pollution levels

    NARCIS (Netherlands)

    Cegłowska, A.; Sokołowska, K.; Samecka-Cymerman, A.; Kolon, K.; Jusik, S.; Kempers, A.J.

    2016-01-01

    The anthropogenic impact of xenobiotics contributes to environmental risk for the aquatic environment and thus, must be controlled. Elodea canadensis, a cosmopolitan aquatic macrophyte with an important role in the ecology of many littoral zones, may provide an integrated record of pollution.

  4. Growth and stocking of eastern hemlock (Tsuga canadensis) in New England

    Science.gov (United States)

    Dale S. Solomon; William B. Leak

    2000-01-01

    Summarization of the limited growth information in mixed-species stands in New England indicates that eastern hemlock (Tsuga canadensis) may be one of the fastest growing species in diameter, second only to white pine. However, on some sites hemlock diameter growth is about equal to that of associated hardwoods. Hemlock grows slowly in height and...

  5. Rhododendron maximum impacts seed bank composition and richness following Tsuga canadensis loss in riparian forests

    Science.gov (United States)

    Tristan M. Cofer; Katherine J. Elliott; Janis K. Bush; Chelcy F. Miniat

    2018-01-01

    Southern Appalachian riparian forests have undergone changes in composition and function from invasive pathogens and pests. Castanea dentata mortality in the 1930s from chestnut blight (Cryphonectria parasitica) and Tsuga canadensis mortality in the 2000s from the hemlock woolly adelgid (Adelges tsugae) have led to the expansion and...

  6. Tsuga canadensis (L.) Carr, mortality will impact hydrologic processes in southern Appalachian forest ecosystems

    Science.gov (United States)

    Chelcy R. Ford; James M. Vose

    2007-01-01

    Eastern hemlock (Tsuga canadensis (L.) Carr.) is one of the principal riparian and cove canopy species in the southern Appalachian Mountains. Throughout its range, eastern hemlock is facing potential widespread mortality from the hemlock woolly adelgid (HWA). If HWA-induced eastern hemlock mortality alters hydrologic function, land managers...

  7. Karyological investigations on Erigeron canadensis L. and E. annuus (L. Pers. from natural stands in Poland

    Directory of Open Access Journals (Sweden)

    K. Bijok

    2015-01-01

    Full Text Available The number of chromosomes in metaphases root meristems of Erigeron canadensis L. (2n= 18 and E. annuus (L. Pers. (2n=27 was determined. The chromosomes of both species showed a morphological differentiation - they were heterobrachial or isobrachial; E. annuus had an additional unpaired isobrachial chromosome.

  8. Secure and documented screwing down of Castor containers

    International Nuclear Information System (INIS)

    Yilmaz, Asir; Junkers, Patrick

    2011-01-01

    The castor container is sealed using a multiple lid system comprising a primary lid and a secondary lid. The two lids are positioned one above the other and screwed securely to the body of the container. In addition, the tightness of the lids is guaranteed by a special metal seal. A further steel plate is screwed securely over the lids providing additional protection for the lids from moisture and various influences. The central element connecting the lids to the container comprises fixed threaded connections which must be accurately tightened with a torque of 900 Nm, 1500 Nm or more. The basic function of a threaded connection, in terms of a positive fit and detachable joint, is to join together two or more parts in such a way that they always behave as a single part whatever operational forces are present. This can only be achieved by means of an accurate and sufficient pre-tensioning force. This pretensioning force is transferred to the threaded connection with the help of a particular torque and presses the components together. This loading brought about by the pretensioning force is referred to as the prestress. In order to create a correct and high quality connection, this prestress must be arrived at in an optimum manner. The prestress must therefore be high enough to withstand the full loading for which the connection is designed. In addition the connection should not come loose unintentionally if it is exposed to externally acting forces. Since under no circumstances should threaded connections on a castor container fail, a suitable screwing process must guarantee that this does not happen. The securing of threaded joints can only be ensured by introducing and maintaining a constant and accurate prestress. VDI Guideline 2230, Part 1, mentions various threaded connection or tightening processes, including torque-controlled tightening, angle-controlled tightening and yield-controlled tightening. (orig.)

  9. Secure and documented screwing down of Castor containers

    Energy Technology Data Exchange (ETDEWEB)

    Yilmaz, Asir; Junkers, Patrick [HYTORC - Barbarino und Kilp GmbH, Krailling (Germany)

    2011-07-01

    The castor container is sealed using a multiple lid system comprising a primary lid and a secondary lid. The two lids are positioned one above the other and screwed securely to the body of the container. In addition, the tightness of the lids is guaranteed by a special metal seal. A further steel plate is screwed securely over the lids providing additional protection for the lids from moisture and various influences. The central element connecting the lids to the container comprises fixed threaded connections which must be accurately tightened with a torque of 900 Nm, 1500 Nm or more. The basic function of a threaded connection, in terms of a positive fit and detachable joint, is to join together two or more parts in such a way that they always behave as a single part whatever operational forces are present. This can only be achieved by means of an accurate and sufficient pre-tensioning force. This pretensioning force is transferred to the threaded connection with the help of a particular torque and presses the components together. This loading brought about by the pretensioning force is referred to as the prestress. In order to create a correct and high quality connection, this prestress must be arrived at in an optimum manner. The prestress must therefore be high enough to withstand the full loading for which the connection is designed. In addition the connection should not come loose unintentionally if it is exposed to externally acting forces. Since under no circumstances should threaded connections on a castor container fail, a suitable screwing process must guarantee that this does not happen. The securing of threaded joints can only be ensured by introducing and maintaining a constant and accurate prestress. VDI Guideline 2230, Part 1, mentions various threaded connection or tightening processes, including torque-controlled tightening, angle-controlled tightening and yield-controlled tightening. (orig.)

  10. Haematology and Serum Biochemistry Parameters and Variations in the Eurasian Beaver (Castor fiber.

    Directory of Open Access Journals (Sweden)

    Simon J Girling

    Full Text Available Haematology parameters (N = 24 and serum biochemistry parameters (N = 35 were determined for wild Eurasian beavers (Castor fiber, between 6 months - 12 years old. Of the population tested in this study, N = 18 Eurasian beavers were from Norway and N = 17 originating from Bavaria but now living extensively in a reserve in England. All blood samples were collected from beavers via the ventral tail vein. All beavers were chemically restrained using inhalant isoflurane in 100% oxygen prior to blood sampling. Results were determined for haematological and serum biochemical parameters for the species and were compared between the two different populations with differences in means estimated and significant differences being noted. Standard blood parameters for the Eurasian beaver were determined and their ranges characterised using percentiles. Whilst the majority of blood parameters between the two populations showed no significant variation, haemoglobin, packed cell volume, mean cell haemoglobin and white blood cell counts showed significantly greater values (p<0.01 in the Bavarian origin population than the Norwegian; neutrophil counts, alpha 2 globulins, cholesterol, sodium: potassium ratios and phosphorus levels showed significantly (p<0.05 greater values in Bavarian versus Norwegian; and potassium, bile acids, gamma globulins, urea, creatinine and total calcium values levels showed significantly (p<0.05 greater values in Norwegian versus Bavarian relict populations. No significant differences were noted between male and female beavers or between sexually immature (<3 years old and sexually mature (≥3 years old beavers in the animals sampled. With Eurasian beaver reintroduction encouraged by legislation throughout Europe, knowledge of baseline blood values for the species and any variations therein is essential when assessing their health and welfare and the success or failure of any reintroduction program. This is the first study to produce

  11. Molecular characterization of Echinococcus granulosus sensu stricto and Echinococcus canadensis in humans and livestock from Algeria.

    Science.gov (United States)

    Zait, Houria; Kouidri, Mokhtaria; Grenouillet, Florence Elisabeth; Umhang, Gérald; Millon, Laurence; Hamrioui, Boussad; Grenouillet, Frédéric

    2016-06-01

    In Algeria, previous studies investigated genotypes of Echinococcus granulosus sensu lato in animals and identified E. granulosus sensu stricto (s.s.) genotypes G1 and G3 whereas Echinococcus canadensis genotype G6 was only reported from dromedary cysts. Molecular data on human cystic echinococcosis (CE) were limited. We implemented a large genotyping study of hydatid cysts from humans and livestock animals to specify CE's molecular epidemiology and the genetic diversity in Algeria. Fifty-four human CE cysts from patients predominantly admitted in surgical units from Mustapha Hospital, Algiers, and 16 cysts from livestock animals gathered in two geographically distinct slaughterhouses, Tiaret and Tamanrasset, were collected. Molecular characterization was performed using sequencing of two mitochondrial genes, cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit I (NDI). In humans, G1 of E. granulosus s.s. was the main genotype (90.7 %); four samples (7.4 %) were characterized as E. granulosus s.s. G3 and one cyst as E. canadensis G6 (1.8 %). This molecular confirmation of E. canadensis G6 human infection in Algeria was observed in a Tuareg female living in a desertic area in Tamanrasset. All cysts from sheep, cattle, and goat were identified as E. granulosus s.s. G1 and the two cysts originating from dromedary as E. canadensis G6. Twenty concatenated haplotypes (COI + NDI) were characterized. Among E. granulosus s.s., one haplotype (HL1) was highly predominant in both humans and animals cysts (71.6 %). This study revealed main occurrence of E. granulosus s.s. in humans and livestock animals, with description of a predominant shared haplotype corresponding to the main worldwide observed haplotype E.granulosus s.s. G1. E. canadensis G6 was limited to South Algeria, in dromedary as well as in human.

  12. The castor bean breeding and the energy substitution; A cultura da mamoneira e a substituicao de energia

    Energy Technology Data Exchange (ETDEWEB)

    Savy Filho, A; Banzatto, N V; Bernardi, J A; Kurachi, S A.H.; Azzini, A [Instituto Agronomico de Campinas, SP (Brazil)

    1985-12-31

    This work introduces castor bean as a source of renewable energy. It identifies the potential of production of oil to be used as fuel. Besides, it makes a survey about the production of raw products for new uses. It also presents the available technology on seed and other materials derived from castor cropping and also showing the potential of castor bean not only with the current cropping systems but also with more specialized practices, such as castor bean crop mechanization. 11 refs., 1 fig., 4 tabs.

  13. Screening of the entire USDA castor germplasm collection for oil content and fatty acid composition for optimum biodiesel production.

    Science.gov (United States)

    Wang, Ming Li; Morris, J Bradley; Tonnis, Brandon; Pinnow, David; Davis, Jerry; Raymer, Paul; Pederson, Gary A

    2011-09-14

    Castor has tremendous potential as a feedstock for biodiesel production. The oil content and fatty acid composition in castor seed are important factors determining the price for production and affecting the key fuel properties of biodiesel. There are 1033 available castor accessions collected or donated from 48 countries worldwide in the USDA germplasm collection. The entire castor collection was screened for oil content and fatty acid composition by nuclear magnetic resonance (NMR) and gas chromatography (GC), respectively. Castor seeds on the average contain 48.2% oil with significant variability ranging from 37.2 to 60.6%. Methyl esters were prepared from castor seed by alkaline transmethylation. GC analysis of methyl esters confirmed that castor oil was composed primarily of eight fatty acids: 1.48% palmitic (C16:0), 1.58% stearic (C18:0), 4.41% oleic (C18:1), 6.42% linoleic (C18:2), 0.68% linolenic (C18:3), 0.45% gadoleic (C20:1), 84.51% ricinoleic (C18:1-1OH), and 0.47% dihydroxystearic (C18:0-2OH) acids. Significant variability in fatty acid composition was detected among castor accessions. Ricinoleic acid (RA) was positively correlated with dihydroxystearic acid (DHSA) but highly negatively correlated with the five other fatty acids except linolenic acid. The results for oil content and fatty acid composition obtained from this study will be useful for end-users to explore castor germplasm for biodiesel production.

  14. Disease and predation: sorting out causes of a bighorn sheep (Ovis canadensis decline.

    Directory of Open Access Journals (Sweden)

    Joshua B Smith

    Full Text Available Estimating survival and documenting causes and timing of mortality events in neonate bighorn sheep (Ovis canadensis improves understanding of population ecology and factors influencing recruitment. During 2010-2012, we captured and radiocollared 74 neonates in the Black Hills, South Dakota, of which 95% (70 died before 52 weeks of age. Pneumonia (36% was the leading cause of mortality followed by predation (30%. We used known fate analysis in Program MARK to estimate weekly survival rates and investigate the influence of intrinsic variables on 52-week survival. Model {S1 wk, 2-8 wks, >8 wks} had the lowest AIC c (Akaike's Information Criterion corrected for small sample size value, indicating that age (3-stage age-interval: 1 week, 2-8 weeks, and >8 weeks best explained survival. Weekly survival estimates for 1 week, 2-8 weeks, and >8 weeks were 0.81 (95% CI = 0.70-0.88, 0.86 (95% CI = 0.81-0.90, and 0.94 (95% CI = 0.91-0.96, respectively. Overall probability of surviving 52 weeks was 0.02 (95% CI = 0.01-0.07. Of 70 documented mortalities, 21% occurred during the first week, 55% during weeks 2-8, and 23% occurred >8 weeks of age. We found pneumonia and predation were temporally heterogeneous with lambs most susceptible to predation during the first 2-3 weeks of life, while the greatest risk from pneumonia occurred from weeks 4-8. Our results indicated pneumonia was the major factor limiting recruitment followed by predation. Mortality from predation may have been partly compensatory to pneumonia and its effects were less pronounced as alternative prey became available. Given the high rates of pneumonia-caused mortality we observed, and the apparent lack of pneumonia-causing pathogens in bighorn populations in the western Black Hills, management activities should be geared towards eliminating contact between diseased and healthy populations.

  15. Disease and predation: Sorting out causes of a bighorn sheep (Ovis canadensis) decline

    Science.gov (United States)

    Smith, Joshua B.; Jenks, Jonathan A.; Grovenburg, Troy W.; Klaver, Robert W.

    2014-01-01

    Estimating survival and documenting causes and timing of mortality events in neonate bighorn sheep (Ovis canadensis) improves understanding of population ecology and factors influencing recruitment. During 2010–2012, we captured and radiocollared 74 neonates in the Black Hills, South Dakota, of which 95% (70) died before 52 weeks of age. Pneumonia (36%) was the leading cause of mortality followed by predation (30%). We used known fate analysis in Program MARK to estimate weekly survival rates and investigate the influence of intrinsic variables on 52-week survival. Model {S1 wk, 2–8 wks, >8 wks} had the lowest AICc (Akaike’s Information Criterion corrected for small sample size) value, indicating that age (3-stage age-interval: 1 week, 2–8 weeks, and >8 weeks) best explained survival. Weekly survival estimates for 1 week, 2–8 weeks, and >8 weeks were 0.81 (95% CI = 0.70–0.88), 0.86 (95% CI = 0.81–0.90), and 0.94 (95% CI = 0.91–0.96), respectively. Overall probability of surviving 52 weeks was 0.02 (95% CI = 0.01–0.07). Of 70 documented mortalities, 21% occurred during the first week, 55% during weeks 2–8, and 23% occurred >8 weeks of age. We found pneumonia and predation were temporally heterogeneous with lambs most susceptible to predation during the first 2–3 weeks of life, while the greatest risk from pneumonia occurred from weeks 4–8. Our results indicated pneumonia was the major factor limiting recruitment followed by predation. Mortality from predation may have been partly compensatory to pneumonia and its effects were less pronounced as alternative prey became available. Given the high rates of pneumonia-caused mortality we observed, and the apparent lack of pneumonia-causing pathogens in bighorn populations in the western Black Hills, management activities should be geared towards eliminating contact between diseased and healthy populations.

  16. Castor oil, bath and/or enema for cervical priming and induction of labour.

    Science.gov (United States)

    Kelly, Anthony J; Kavanagh, Josephine; Thomas, Jane

    2013-07-24

    Castor oil, a potent cathartic, is derived from the bean of the castor plant. Anecdotal reports, which date back to ancient Egypt have suggested the use of castor oil to stimulate labour. Castor oil has been widely used as a traditional method of initiating labour in midwifery practice. Its role in the initiation of labour is poorly understood and data examining its efficacy within a clinical trial are limited. This is one of a series of reviews of methods of cervical ripening and labour induction using standardised methodology. To determine the effects of castor oil or enemas for third trimester cervical ripening or induction of labour in comparison with other methods of cervical ripening or induction of labour. We searched the Cochrane Pregnancy and Childbirth Group's Trials Register (30 April 2013) and bibliographies of relevant papers. Clinical trials comparing castor oil, bath or enemas used for third trimester cervical ripening or labour induction with placebo/no treatment or other methods listed above it on a predefined list of labour induction methods. A strategy was developed to deal with the large volume and complexity of trial data relating to labour induction. This involved a two-stage method of data extraction. Three trials, involving 233 women, are included. There was no evidence of differences in caesarean section rates between the two interventions in the two trials reporting this outcome (risk ratio (RR) 2.04, 95% confidence interval (CI) 0.92 to 4.55). There were no data presented on neonatal or maternal mortality or morbidity.There was no evidence of a difference between castor oil and placebo/no treatment for the rate of instrumental delivery, meconium-stained liquor, or Apgar score less than seven at five minutes. The number of participants was too small to detect all but large differences in outcome. All women who ingested castor oil felt nauseous (RR 59.92, 95% CI 8.46 to 424.52). The three trials included in the review contain small numbers

  17. Haemosporida prevalence and diversity are similar in endangered wild whooping cranes (Grus americana) and sympatric sandhill cranes (Grus canadensis).

    Science.gov (United States)

    Bertram, Miranda R; Hamer, Gabriel L; Hartup, Barry K; Snowden, Karen F; Medeiros, Matthew C; Hamer, Sarah A

    2017-04-01

    The population growth of endangered whooping cranes (Grus americana) is not consistent with species recovery goals, and the impact of parasite infection on whooping crane populations is largely unknown. Disease ecology and epidemiology research of endangered species is often hindered by limited ability to conduct invasive sampling on the target taxa. Accordingly, we hypothesized that sandhill cranes (Grus canadensis) would be a useful surrogate species to investigate the health impacts of Haemosporida infection in whooping cranes. Our goal was to compare the prevalence and diversity of Haemosporida infection between whooping cranes and sandhill cranes. We detected an overall infection prevalence of 83·6% (n = 61) in whooping cranes and 59·6% (n = 47) and 63·6 (n = 22) in two sympatric sandhill crane populations captured in Texas. Prevalence was significantly lower in allopatric sandhill cranes captured in New Mexico (12·1%, n = 33). Haemoproteus antigonis was the most abundant haemoparasite in cranes, present in 57·4% of whooping cranes and 39·2% of sandhill cranes; Plasmodium and Leucocytozoon were present at significantly lower levels. The high prevalence of Haemosporida in whooping cranes and sympatric sandhill cranes, with shared parasite lineages between the two species, supports sandhill cranes as a surrogate species for understanding health threats to endangered whooping cranes.

  18. Interactions between Rocky Mountain Bighorn Sheep Ovis canadensis canadensis and Domestic Sheep Ovis aries and the Biological, Social, Economic, and Legal Implications of these Interactions on USDA Forest Service Lands in the Evanston/Mt. View Ranger District

    OpenAIRE

    Herrera, Ashly Nicole

    2012-01-01

    Strong evidence exists indicating domestic sheep (Ovis aries) can infect Rocky Mountain bighorn sheep (Ovis canadensis canadensis), a United States Forest Service (USFS) Region 4 sensitive species, with pneumonia (Callan 1991; Foreyt 1989, 1992, 1994; Foreyt and Lagerquist 1996; George et al 2008; Wehausen et al. 2011). Since the transmission of the pneumonic bacteria between the domestic and wild sheep is a result of bighorn sheep coming into contact with the bacteria carried in the mucous m...

  19. First molecular evidence of the simultaneous human infection with two species of Echinococcus granulosus sensu lato: Echinococcus granulosus sensu stricto and Echinococcus canadensis.

    Science.gov (United States)

    Oudni-M'rad, Myriam; M'rad, Selim; Ksia, Amine; Lamiri, Rachida; Mekki, Mongi; Nouri, Abdellatif; Mezhoud, Habib; Babba, Hamouda

    2016-03-01

    Cystic echinococcosis is a widespread zoonotic parasitic disease especially in Tunisia which is one of the most endemic countries in the Mediterranean area. The etiological agent, Echinococcus granulosus sensu lato, implies dogs and other canids as definitive hosts and different herbivore species as intermediate hosts. Human contamination occurs during the consumption of parasite eggs passed in the environment through canid feces. Hydatid cysts coming from a child operated for multiple echinococcosis were collected and analyzed in order to genotype and to obtain some epidemiological molecular information. Three targets, ribosomal DNA ITS1 fragment, NADH dehydrogenase subunit 1 (nad1), and mitochondrial cytochrome c oxydase subunit 1 (CO1) genes, were amplified and analyzed by RFLP and sequencing approach. This study presents the first worldwide report in human of a simultaneous infection with Echinococcus granulosus sensu stricto (genotype G1) and Echinococcus canadensis (genotype G6) species. This is also the first report of the presence of E. canadensis in the Tunisian population which argues in favor of a greater importance of this species in human infestation in Tunisia than previously believed.

  20. Landscape location affects genetic variation of Canada lynx (Lynx canadensis)

    Science.gov (United States)

    M. K. Schwartz; L. S. Mills; Y. Ortega; L. F. Ruggiero; F. W. Allendorf

    2003-01-01

    The effect of a population's location on the landscape on genetic variation has been of interest to population genetics for more than half a century. However, most studies do not consider broadscale biogeography when interpreting genetic data. In this study, we propose an operational definition of a peripheral population, and then explore whether peripheral...

  1. THE EFFECTS OF POSSIBLE CONTAMINATION ON THE RADIOCARBON DATING OF THE DEAD SEA SCROLLS II : EMPIRICAL METHODS TO REMOVE CASTOR OIL AND SUGGESTIONS FOR REDATING

    NARCIS (Netherlands)

    Rasmussen, Kaare Lund; van der Plicht, Johannes; Doudna, Gregory; Nielsen, Frederik; Hojrup, Peter; Stenby, Erling Halfdan; Pedersen, Carl Th; Højrup, Peter

    2009-01-01

    While kept at the Rockefeller Museum in East Jerusalem, many Dead Sea Scroll fragments were exposed to castor oil by the original team of editors in the course of cleaning the parchments. Castor oil must be regarded as a serious contaminant in relation to radiocarbon dating. If modern castor oil is

  2. Identification of diacylglycerol and triacylglycerols containing 11,12,13-trihydroxy-9,14-octadecadienoic acid in castor oil by mass spectrometry

    Science.gov (United States)

    Castor oil has many industrial uses. Molecular species of acylglycerols containing monohydroxy, dihydroxy and trihydroxy fatty acids in castor oil have been reported. The identification of acylglycerols containing a triOH18:2 fatty acid in castor oil is reported here. The structure of this novel fat...

  3. Castor oil as a natural alternative to labor induction: A retrospective descriptive study.

    Science.gov (United States)

    DeMaria, Andrea L; Sundstrom, Beth; Moxley, Grace E; Banks, Kendall; Bishop, Ashlan; Rathbun, Lesley

    2018-04-01

    To describe birthing outcomes among women who consumed castor oil cocktail as part of a freestanding birth center labor induction protocol. De-identified data from birth logs and electronic medical records were entered into SPSS Statistics 22.0 for analysis for all women who received the castor oil cocktail (n=323) to induce labor between January 2008 and May 2015 at a birth center in the United States. Descriptive statistics were analyzed for trends in safety and birthing outcomes. Of the women who utilized the castor oil cocktail to stimulate labor, 293 (90.7%) birthed vaginally at the birth center or hospital. The incidence of maternal adverse effects (e.g., nausea, vomiting, extreme diarrhea) was less than 7%, and adverse effects of any kind were reported in less than 15% of births. An independent sample t-test revealed that parous women were more likely to birth vaginally at the birth center after using the castor oil cocktail than their nulliparous counterparts (poil cocktail to induce labor were able to give birth vaginally with little to no maternal or fetal complications. Findings indicate further research is needed to compare the safety and effectiveness of natural labor induction methodologies, including castor oil, to commonly used labor induction techniques in a prospective study or clinical trial. Copyright © 2017 Australian College of Midwives. Published by Elsevier Ltd. All rights reserved.

  4. Environmental effects of nanosilver: impact on castor seed germination, seedling growth, and plant physiology.

    Science.gov (United States)

    Yasur, Jyothsna; Rani, Pathipati Usha

    2013-12-01

    Increasing use of nanoparticles in daily products is of great concern today, especially when their positive and negative impact on environment is not known. Hence, in current research, we have studied the impact of silver nanoparticle (AgNPs) and silver nitrate (AgNO3) application on seed germination, root, and shoot length of castor bean, Ricinus communis L. plant. Silver nanoparticles had no significant effects on seedling growth even at higher concentration of 4,000 mg L(-1), while the silver in bulk form as AgNO3 applied on the castor bean seeds inhibited the seed germination. Silver uptake in seedlings of the castor seeds on treatment with both the forms of silver was confirmed through atomic absorption spectroscopy studies. The silver nanoparticle and silver nitrate application to castor seeds also caused an enhanced enzymatic activity of ROS enzymes and phenolic content in castor seedlings. High-performance liquid chromatography analysis of individual phenols indicated enhanced content of parahydroxy benzoic acid. These kinds of studies are of great interest in order to unveil the movement and accumulation of nanoparticles in plant tissues for assessing future applications in the field or laboratory.

  5. Prospects of castor (Ricinus communis L.) genotypes for biodiesel production in India

    International Nuclear Information System (INIS)

    Lavanya, C.; Murthy, I.Y.L.N.; Nagaraj, G.; Mukta, N.

    2012-01-01

    The search for suitable non-edible oilseed crops for production of biodiesel has led to exploration of the potential of castor, hitherto an export oriented commercial oilseed crop in India. In this context, a study was conducted to identify high yielding castor genotypes ideal for biodiesel production. The material evaluated included seed of 15 castor genotypes grown in rainfed conditions of Alfisols at Hyderabad, India. Variability for palmitic, stearic, oleic, linoleic fatty acids was recorded. Ricinoleic acid, the predominant mono unsaturated fatty acid varied among castor genotypes from 86.7 to 92.1%. Correlation coeffecients between fatty acid profile and biodiesel traits were computed. Genotypes 48-1 and DCH-200 exhibited high O/L ratio, low Iodine value (IV) and high cetane number (CN) which indicates higher stability, longer shelf life, quick ignition and greater combustion quality. Genotype DPC-9 exhibited potential as a female parent for development of biodiesel suitable hybrid. -- Highlights: ► Evaluated 15 castor genotypes for fatty acid profile. ► Computed biodiesel related traits like saponification number, iodine value and cetane number. ► Ricinoleic acid, the predominant mono unsaturated fatty acid ranged from 86.7 to 92.1%. ► Variety 48-1 and hybrid DCH-200 exhibited high O/L ratio, low iodine value (IV) and high cetane number (CN).

  6. CASTOR BEAN AND SUNFLOWER INTERCROPPING SYSTEMS IN ROW ARRANGEMENT: BIOLOGICAL EFFICIENCY

    Directory of Open Access Journals (Sweden)

    Ciro de Miranda Pinto

    2012-07-01

    Full Text Available An experiment field was carried in the agricultural seasons 2008, 2009 and 2010, with aim of studying the response of castorbean (Ricinus communis L. intercropping with sunflower (Helianthus annus L. in row arrangement in the dryland farming conditions. In addition, it was evaluated the biological efficiency of plants in intercropping systems.The design used in the experiment was randomized block with seven treatement and four replications. The treatments were represented by rows of castor oil (Ma and sunflower (Gi listed below: 1Ma:1Gi; 1Ma:2Gi; 1Ma:3Gi; 2Ma:2Gi; 2Ma:3Gi; castor and sunflower in the monoculture. The efficiency of intercropping was measured by LER, ATER, LEC, average between LER and ATER, SPI and CoR. The grain yield of castor bean and sunflower were reduced in intercropped row arrangements. The row arrangement 1Ma:2Gi showed the smallest reduction of average productivity of castor beans and sunflower in the evaluation period of the experiment. The castor bean was the dominant crop in relation to sunflower.

  7. Effectiveness of castor oil extract on Escherichia coli and its endotoxins in root canals.

    Science.gov (United States)

    Valera, Marcia Carneiro; Maekawa, Lilian Eiko; Chung, Adriana; de Oliveira, Luciane Dias; Carvalho, Claudio Antonio Talge; Koga-Ito, Cristiane Yumi; Jorge, Antonio Olavo Cardoso

    2012-01-01

    This in vitro study sought to evaluate the effectiveness of castor oil extract used as an irrigating solution on Escherichia coli and its endotoxins in root canals. Sixty single-rooted teeth were prepared (using castor oil extract as irrigating solution) and divided into five groups (n = 12): Group 1 samples were treated with calcium hydroxide (Ca(OH)2), Group 2 samples were treated with polymyxin B, Group 3 samples were treated with Ca(OH)2 and 2% chlorhexidine gel (CHX), and Group 4 samples were treated with castor oil extract. A control group used physiological saline solution as an irrigant. Canal content samples were collected at four different times: immediately after instrumentation, seven days after instrumentation, after 14 days of intracanal medication, and seven days after removal of intracanal medication. A plating method was used to assess antimicrobial activity and the quantification of endotoxins was evaluated by the chromogenic Limulus lysate assay. Data were submitted to ANOVA and a Dunn test (a = 5%). Irrigation with castor oil extract decreased E. coli counts but had no effect on the level of endotoxins. Samples taken seven days after removal of medication revealed a significant reduction in endotoxin levels in Groups 3 and 4. Compared to the saline solution irrigation, castor oil extract decreased microorganism counts in root canals immediately after canal preparation. None of the medications used completely eliminated endotoxins in the root canal.

  8. Optimization of biodiesel production from castor oil using response surface methodology.

    Science.gov (United States)

    Jeong, Gwi-Taek; Park, Don-Hee

    2009-05-01

    The short supply of edible vegetable oils is the limiting factor in the progression of biodiesel technology; thus, in this study, we applied response surface methodology in order to optimize the reaction factors for biodiesel synthesis from inedible castor oil. Specifically, we evaluated the effects of multiple parameters and their reciprocal interactions using a five-level three-factor design. In a total of 20 individual experiments, we optimized the reaction temperature, oil-to-methanol molar ratio, and quantity of catalyst. Our model equation predicted that the following conditions would generate the maximum quantity of castor biodiesel (92 wt.%): a 40-min reaction at 35.5 degrees C, with an oil-to-methanol molar ratio of 1:8.24, and a catalyst concentration of 1.45% of KOH by weight of castor oil. Subsequent empirical analyses of the biodiesel generated under the predicted conditions showed that the model equation accurately predicted castor biodiesel yields within the tested ranges. The biodiesel produced from castor oil satisfied the relevant quality standards without regard to viscosity and cold filter plugging point.

  9. Shared Bacterial and Viral Respiratory Agents in Bighorn Sheep (Ovis canadensis, Domestic Sheep (Ovis aries, and Goats (Capra hircus in Montana

    Directory of Open Access Journals (Sweden)

    David S. Miller

    2011-01-01

    Full Text Available Transmission of infectious agents from livestock reservoirs has been hypothesized to cause respiratory disease outbreaks in bighorn sheep (Ovis canadensis, and land management policies intended to limit this transmission have proven controversial. This cross-sectional study compares the infectious agents present in multiple populations of bighorn sheep near to and distant from their interface with domestic sheep (O. aries and domestic goat (Capra hircus and provides critical baseline information needed for interpretations of cross-species transmission risks. Bighorn sheep and livestock shared exposure to Pasteurellaceae, viral, and endoparasite agents. In contrast, although the impact is uncertain, Mycoplasma sp. was isolated from livestock but not bighorn sheep. These results may be the result of historic cross-species transmission of agents that has resulted in a mosaic of endemic and exotic agents. Future work using longitudinal and multiple population comparisons is needed to rigorously establish the risk of outbreaks from cross-species transmission of infectious agents.

  10. Shared bacterial and viral respiratory agents in bighorn sheep (Ovis canadensis), domestic sheep (Ovis aries), and goats (Capra hircus) in Montana

    Science.gov (United States)

    Miller, David S.; Weiser, Glen C.; Aune, Keith; Roeder, Brent; Atkinson, Mark; Anderson, Neil; Roffe, Thomas J.; Keating, Kim A.; Chapman, Phillip L.; Kimberling, Cleon; Rhyan, Jack C.; Clarke, P. Ryan

    2011-01-01

    Transmission of infectious agents from livestock reservoirs has been hypothesized to cause respiratory disease outbreaks in bighorn sheep (Ovis canadensis), and land management policies intended to limit this transmission have proven controversial. This cross-sectional study compares the infectious agents present in multiple populations of bighorn sheep near to and distant from their interface with domestic sheep (O. aries) and domestic goat (Capra hircus) and provides critical baseline information needed for interpretations of cross-species transmission risks. Bighorn sheep and livestock shared exposure to Pasteurellaceae, viral, and endoparasite agents. In contrast, although the impact is uncertain, Mycoplasma sp. was isolated from livestock but not bighorn sheep. These results may be the result of historic cross-species transmission of agents that has resulted in a mosaic of endemic and exotic agents. Future work using longitudinal and multiple population comparisons is needed to rigorously establish the risk of outbreaks from cross-species transmission of infectious agents.

  11. The castor oil production in Brazil and the biodiesel program (Probiodiesel); A producao de mamona no Brasil e o Probiodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Cezar Menezes; Almeida Neto, Jose Adolfo de; Pires, Monica de Moura [Universidade Estadual de Santa Cruz, Ilheus, BA (Brazil). Grupo Bioenergia e Meio Ambiente]. E-mails: biocomb@uesc.br; Rocha, Pauletti Karllien [Secretaria de Ciencia, Tecnologia e Inovacao do Estado da Bahia (SECTI), Salvador, BA (Brazil)]. E-mail: pauletti@secti.ba.gov.br

    2004-07-01

    The objective of this work is to present a prospective study of the national production of biodiesel and castor oil and to determine the level of the public investment necessary to attain the proposed production. For replacement of 2% of the diesel internal consumption, 786 millions of liters of biodiesel will be necessary, based on the consumption of 2003. From this amount 293 millions of liters (40%) will be derived from the castor oil. Considering the castor oil agricultural productivity of 1.8 t/ha and the 45% industrial income in oil, it will be necessary the planting of 360.000 ha and investment amount of R$ 370 million. An idleness of 71% of the castor oil crushing industry has been detected, but this sector works with profit as function of castor oil price. An deficit of 55% between the castor oil planting area and the necessary one for biodiesel production was identified, considering that all the castor oil will be exclusively used for this purpose. With the proposed goals and without the necessary allocation of public resources for castor oil production, probably it will not be possible to expect that 1/5 of the biodiesel national production can come from the domestic agricultural planting.

  12. Biosynthesis of the sesquiterpene germacrene D in Solidago canadensis: 13C and (2)H labeling studies.

    Science.gov (United States)

    Steliopoulos, Panagiotis; Wüst, Matthias; Adam, Klaus-Peter; Mosandl, Armin

    2002-05-01

    The biogenetic origin of the isoprenoid building blocks of the sesquiterpene germacrene D was studied in Solidago canadensis. Feeding experiments were carried out with 1-[5,5-D(2)]deoxy-D-xylulose-5-phosphate (D(2)-DOXP), [5-13C]mevalonolactone (13C-MVL) and [1-13C]-D-glucose. The hydrodistillate of a cut shoot fed with D(2)-DOXP was investigated by enantio-MDGC-MS and the volatile fraction of a shoot supplied with 13C-MVL was examined by GC-C-IRMS. The incorporation of [1-13C]-D-glucose was analyzed by quantitative 13C NMR spectroscopy after isolation of germacrene D from the essential oil. Our labeling studies revealed that the biosynthesis of the C-15 skeleton of sesquiterpene germacrene D in Solidago canadensis proceeds predominantly via the methylerythritol phosphate pathway.

  13. Patch colonization by Trirhabda canadensis (Coleoptera: Chrysomelidae): effects of plant species composition and wind.

    Science.gov (United States)

    Morrow, P A; Tonkyn, D W; Goldburg, R J

    1989-10-01

    The goldenrod leaf beetle, Trirhabda canadensis, is known to respond to odors of host and non-host species in the laboratory. Here we report movements of T. canadensis in the field in response to volatile odors from monocultures and polycultures of host plants. Overall, beetles preferentially colonized plots with a higher density of host plants and lower diversity of allelochemicals, but under some wind conditions there were marked exceptions. At high windspeeds, they colonized whichever plot(s) was upwind. At low windspeeds, beetles colonized preferred plots even when they were not upwind. The data suggest that odor dispersion varies in a complex way with windspeed: at low windspeeds beetles received information from a wide are of vegetation and made choices while at high windspeeds information was available only from upwind plot(s).

  14. Streamlining CASTOR to manage the LHC data torrent

    International Nuclear Information System (INIS)

    Presti, G Lo; Curull, X Espinal; Cano, E; Fiorini, B; Ieri, A; Murray, S; Ponce, S; Sindrilaru, E

    2014-01-01

    This contribution describes the evolution of the main CERN storage system, CASTOR, as it manages the bulk data stream of the LHC and other CERN experiments, achieving over 90 PB of stored data by the end of LHC Run 1. This evolution was marked by the introduction of policies to optimize the tape sub-system throughput, going towards a cold storage system where data placement is managed by the experiments' production managers. More efficient tape migrations and recalls have been implemented and deployed where bulk meta-data operations greatly reduce the overhead due to small files. A repack facility is now integrated in the system and it has been enhanced in order to automate the repacking of several tens of petabytes, required in 2014 in order to prepare for the next LHC run. Finally the scheduling system has been evolved to integrate the internal monitoring. To efficiently manage the service a solid monitoring infrastructure is required, able to analyze the logs produced by the different components (about 1 kHz of log messages). A new system has been developed and deployed, which uses a transport messaging layer provided by the CERN-IT Agile Infrastructure and exploits technologies including Hadoop and HBase. This enables efficient data mining by making use of MapReduce techniques, and real-time data aggregation and visualization. The outlook for the future is also presented. Directions and possible evolution will be discussed in view of the restart of data taking activities.

  15. Transesterification of Castor Oil Catalyzed by Liquid Enzymes

    DEFF Research Database (Denmark)

    Andrade, Thalles Allan; Errico, Massimiliano; Christensen, Knud Villy

    2017-01-01

    economy. Based on this, and considering its low influence with food production, castor oil was investigated as a potential feedstock. Compared to other vegetable oils, it has a higher polarity resulting in better system homogeneity during reaction. The enzyme tested as catalyst was Eversa Transform. Four...... main reaction parameters were investigated for the optimization of the reaction route: the temperature was varied from 35 to 45 °C, the water content between 0-10 wt%, the enzyme content in the range of 2-10 wt%, and the alcohol-to-oil molar ratio from 4.5 to 7.5. The Response Surface Methodology...... was used to determine the optimal reaction conditions to get a high biodiesel yield and a low free fatty acids concentration. The results obtained showed that at 35 °C, 5 wt% of enzymes, 5 wt% of water, and a 6.0 alcohol-to-oil molar ratio, the yield in biodiesel was about 94% with a content of free fatty...

  16. The performance of the CASTOR calorimeter during LHC Run 2

    CERN Document Server

    van de Klundert, Merijn H F

    2016-01-01

    CASTOR is an electromagnetic and hadronic tungsten-quartz sampling Cerenkov calorimeter located at the Compact Muon Solenoid experiment at the Large Hadron Collider. The detector has pseudorapidity borders at -5.2 and -6.6. An overview is presented on the various aspects of CASTOR’s performance and their relations during LHC Run 2. The equalisation of CASTOR’s channels is performed using beam-halo muons. Thereafter, CASTOR’s pedestal spectrum is studied. It is shown that noise estimates which are extracted using a fit, give on average a 10% lower threshold than statistical estimates. Gain correction factors, which are needed for the intercalibration, are obtained using a statistical, in-situ applicable method. The results of this method are shown to be reasonably consistent with laboratory measurements. Penultimately the absolute calibration is discussed, with emphasis on the relation between the scale uncertainty and CASTOR’s alignment. It is shown that the alignment’s contribution to the systemati...

  17. Morphology and viability of castor bean genotypes pollen grains

    Directory of Open Access Journals (Sweden)

    Maria Selma Alves Silva Diamantino

    2016-01-01

    Full Text Available The objective of this work was to characterize the morphology and viability of the pollen of 15 genotypes of castor bean (Ricinus communis L. and to generate information that can assist in the selection of highly promising male parents for future use in genetic improvement programs aimed at producing seeds for oil extraction. Acetolysis and scanning electron microscopy was used to characterize the morphology of the pollen. The viability of the pollen grains was estimated by in vitro germination and colorimetric analysis (acetocarmine 2% and 2, 3, 5-triphenyltetrazolium chloride 1%. For the in vitro germination, pollen grains were grown in 10 types of solidified culture medium consisting of different concentrations of sucrose, boric acid, calcium nitrate, magnesium sulfate and potassium nitrate. The pollen grains had the following characteristics: medium size, isopolar and subspheroidal shape, radial symmetry, circular ambit, 3-colporate, elongated endoapertures, tectate exine and granulated sexine. The acetocarmine dye overestimated pollen viability. The media M5 and M8 were the most efficient at promoting the germination of pollen grains. The studied genotypes had high levels of viability and can therefore be used as male parents in genetic improvement programs.

  18. Identification of diacylglycerol and triacylglycerol containing 11,12,13-trihydroxy-9,14-octadecadienoic acid in castor oil.

    Science.gov (United States)

    Lin, Jiann-Tsyh; Chen, Grace Q

    2011-02-28

    Castor oil has many industrial uses. Molecular species of acylglycerols containing monohydroxy, dihydroxy and trihydroxy fatty acids in castor oil have been reported. We report here the identification of acylglycerols containing a triOH18:2 fatty acid in castor oil. The structure of this novel fatty acid was proposed as 11,12,13-trihydroxy-9,14-octadecadienoic acid by the mass spectrometry of the lithiated adducts of acylglycerols in the HPLC fractions of castor oil. The fragmentation pathways of the lithiated adduct of 11,12,13-trihydroxy-9,14-octadecadienoic acid were proposed. We also proposed the biosynthetic pathways of polyhydroxy fatty acids in castor. Published by Elsevier B.V.

  19. Evidence for Enhanced Mutualism Hypothesis: Solidago canadensis Plants from Regular Soils Perform Better

    OpenAIRE

    Sun, Zhen-Kai; He, Wei-Ming

    2010-01-01

    The important roles of plant-soil microbe interactions have been documented in exotic plant invasion, but we know very little about how soil mutualists enhance this process (i.e. enhanced mutualism hypothesis). To test this hypothesis we conducted two greenhouse experiments with Solidago canadensis (hereafter Solidago), an invasive forb from North America, and Stipa bungeana (hereafter Stipa), a native Chinese grass. In a germination experiment, we found soil microbes from the rhizospheres of...

  20. Chemical constituents from Solidago canadensis with hypolipidemic effects in HFD-fed hamsters.

    Science.gov (United States)

    Huang, Ying; Hao, Yan-Li; Mai, Xiao-Yan

    2013-01-01

    Two new compounds, 8-dehydroxymethylvisanol (1) and 9-O-[3-O-acetyl-β-d-glucopyranosyl]-4-hydroxy-cinnamic acid (4), together with two known lignans, visanol (2) and 9-aldehydevibsanol (3), were isolated from the 80% EtOH extract of Solidago canadensis. The structures of the two new compounds were elucidated on the basis of 1D, 2D NMR, and mass spectral analysis. All the lignans exhibited moderate hypolipidemic activity in high-fat diet-fed hamsters.

  1. Cyto-histological processes during the differentiation of the tapetal cells in the anthers of Anthemis tinctoria L., Erigeron annuus (L. Pers. and E. canadensis L.

    Directory of Open Access Journals (Sweden)

    K. Bijok

    2015-01-01

    Full Text Available Tapetal cells in the anthers of Anthemis tinctoria, Erigeron annus and E. canadensis were uninucleate in the premeiotic stage of the PMC's. Ta-petum differentiation with inhibited cytokinesis took place during the period of meiotic divisions. In E. canadensis both mitoses were normal, wheareas in A. tinctoria and E. canadensis after both first and second mitotic divisions as a result of numerous disturbances polyploid nuclei were formed.

  2. Organic matter characterization and decomposition dynamics in sub-Antarctic streams impacted by invasive beavers Caracterización de la materia orgánica y la dinámica de descomposición en arroyos subantárticos impactados por castores invasores

    Directory of Open Access Journals (Sweden)

    Erica Ulloa

    2012-11-01

    Full Text Available Despite being a relatively remote and well conserved area, the sub-Antarctic ecoregion faces pressing global threats from climate change, the ozone hole and introduced species. Its freshwater ecosystems are one of the least studied components of this biome, but they are known to confront a host of invasive taxa including trout and beavers. We set out to understand the basic characterization and dynamics of organic matter processing and decomposition in sub-Antarctic streams under natural forest (NF conditions and in ponds constructed by North American beavers (Castor canadensis (BP. We found these streams have a naturally stable benthic organic matter regime throughout the year with a peak in leaf input from Nothofagus pumilio in autumn. Beaver ponds significantly increased the retention of organic matter and caused significantly higher decomposition rates, probably associated with increased density and biomass of Hyalella spp. As expected, leaf decay rates for N. pumilio, a deciduous species, were higher (NF: -0.0028 day- ± 0.0001 SE; BP: -0.0118 day-1 ± 0.0009 SE than N. betuloides (a broad-leaf evergreen (NF: -0.0018 day-1 ± 0.0005 SE; BP: -0.0040 day-1 ± 0.0003 SE. Overall these results indicate that the naturally low decomposition rates (slower than 89% of a global survey of decay rates for these cold, oligotrophic streams are being modified by introduced beavers to resemble more temperate latitudes.A pesar de ser una zona relativamente remota y bien conservado, la ecorregión subantártica se enfrenta a presiones por amenazas globales por el cambio climático, el agujero de ozono y las especies introducidas. Los ecosistemas de agua dulce son uno de los componentes menos estudiados de este bioma, pero se sabe que enfrentan una serie de taxones invasivos como la trucha y los castores. El propósito de este estudio es entender la caracterización básica y dinámica de descomposición de la materia orgánica en arroyos subantárticos de

  3. A microanatomical and histological study of the postcranial dermal skeleton of the Devonian actinopterygian Cheirolepis canadensis

    Directory of Open Access Journals (Sweden)

    Louise Zylberberg

    2016-06-01

    Full Text Available The Devonian stem-actinoterygian Cheirolepis canadensis is potentially important to understand the evolution of the dermal skeleton of osteichthyans, but the last detailed histological study on this taxon was published more than forty years ago. Here, we present new data about the morphology and the histological structure of scales, fulcra, and fin-rays in the Devonian actinopterygian Cheirolepis canadensis through SEM and photomicroscopy. The scales have a typical palaeoniscoid organisation, with ganoine layers overlaying dentine and a bony basal plate, but the ganoine surface lacks the characteristic microtubercles that have been described on the ganoine surface of the scales of polypterids and many other actinopterygians. Fin-rays are composed of segmented and ramified lepidotrichia that show a structure reminiscent of scales, with ganoine and dentine components lying on a thick bony base. We describe articular processes between lepidotrichia that are reminiscent of, and plausibly homologous with, the peg-and-socket articulations between the scales. The analysis of the postcranial dermal skeleton of Cheirolepis canadensis shows that structural similarities between scales and lepidotrichia of this basal actinopterygian are greater than in more recent actinopterygians. The new data on histological and microanatomical structure of the dermal skeleton lend additional support to the hypothesis that lepidotichia are derivatives of scales, though they are also compatible with the more general hypothesis that scales, lepidotrichia and fulcra belong to the same morphogenetic system.

  4. Characterization of terpenoid volatiles from cultivars of eastern hemlock (Tsuga canadensis).

    Science.gov (United States)

    Lagalante, Anthony F; Montgomery, Michael E; Calvosa, Frank C; Mirzabeigi, Michael N

    2007-12-26

    The volatile terpenoid fraction from needles in 13 cultivars of Tsuga canadensis L. (Carriere) was analyzed by gas chromatography with mass spectrometry (GC-MS). The results of this study are considered along with previously reported results for foliar terpenoid levels of the Asian (T. sieboldii, T. chinensis, T. diversifolia), western North American (T. mertensiana, T. heterophylla), and eastern North American species (T. canadensis, T. caroliniana) of hemlock to draw conclusions about the potential of cultivar host resistance to the hemlock woolly adelgid (Adelges tsugae Annand). It is suggested that hemlocks in eastern North America have adapted their terpenoid chemistry for protection against endemic defoliators and that this has made them vulnerable to non-native, sucking pests such as adelgids and scales. Some cultivars of T. canadensis have a terpenoid profile that resembles that of the resistant noneastern North American species and are candidates for biological screening for resistance. Among the cultivars, the variation in terpenoid chemistry did not absolutely correspond with the considerable differences in morphological characters observed, indicating that the terpenoid chemistry is not definitively coupled with hemlock morphology.

  5. Assessing Timing and Causes of Neonatal Lamb Losses in a Bighorn Sheep ( Ovis canadensis canadensis ) Herd via Use of Vaginal Implant Transmitters.

    Science.gov (United States)

    Grigg, Jamin L; Wolfe, Lisa L; Fox, Karen A; Killion, Halcyon J; Jennings-Gaines, Jessica; Miller, Michael W; P Dreher, Brian

    2017-07-01

    We evaluated the use of vaginal implant transmitters (VITs) as a means of detecting, capturing, and radio collaring Rocky Mountain bighorn sheep ( Ovis canadensis canadensis) lambs to estimate survival and to facilitate carcass recovery to assess causes of mortality. We focused on one of several bighorn herds in Colorado, US, suffering from depressed recruitment that was not preceded by a classic all-age die-off. We captured, radio-collared, diagnosed pregnancy by ultrasound examination, and inserted VITs into 15 pregnant ewes from a herd residing near Granite, Colorado. We were subsequently able to collar a lamb from each of 13 VITs, and two additional lambs opportunistically from ewes without transmitters. As lambs died, we recovered and submitted carcasses for necropsy and laboratory assessment. All lambs captured and one additional lamb (carcass found opportunistically) were dead by about 130 d of age: 11 died of apparent pneumonia (all within 8-10 wk of age), one died from trauma after being kicked or trampled, one was killed by a mountain lion ( Puma concolor ), and three died of starvation likely caused by abandonment after capture. Pneumonic lambs had involvement of Mycoplasma ovipneumoniae and leukotoxigenic Bibersteinia trehalosi . The use of VITs and lamb collars enabled us to efficiently identify pneumonia as the predominant cause of depressed lamb recruitment in this herd; however, we urge care in neonatal lamb handling to minimize abandonment.

  6. Thermophysical properties of biodiesel and related systems: (Liquid + liquid) equilibrium data for castor oil biodiesel

    International Nuclear Information System (INIS)

    Mazutti, Marcio A.; Voll, Fernando A.P.; Cardozo-Filho, Lúcio; Corazza, Marcos L.; Lanza, Marcelo; Priamo, Wagner L.; Oliveira, J. Vladimir

    2013-01-01

    Highlights: ► (Liquid + liquid) equilibrium data for multicomponent castor oil FAME and FAEE castor oil. ► Tie-lines and solubility curves (binodal) by cloud-point method for FAME and FAEE systems. ► Experimental data correlated using the UNIQUAC model. -- Abstract: This work reports new liquid–liquid solubility values (binodal curves) as well as (liquid + liquid) equilibrium data for, ternary and quaternary systems containing fatty acid methyl esters (FAME) and fatty acid ethyl esters (FAEE) from castor oil, water, glycerol, methanol and anhydrous ethanol at T = (303.15, 318.15, and 333.15) K. Solubility curves (binodal) were also obtained by the cloud-point method for binary systems containing FAME, FAEE, water, or glycerol. All results obtained can be considered of good quality. The experimental values were correlated using the UNIQUAC model, whose results presented good performance and satisfactory fitting of equilibrium values

  7. Study on Emission and Performance of Diesel Engine Using Castor Biodiesel

    Directory of Open Access Journals (Sweden)

    Md. Saiful Islam

    2014-01-01

    performance of diesel engine using the castor biodiesel and its blend with diesel from 0% to 40% by volume. The acid-based catalyzed transesterification system was used to produce castor biodiesel and the highest yield of 82.5% was obtained under the optimized condition. The FTIR spectrum of castor biodiesel indicates the presence of C=O and C–O functional groups, which is due to the ester compound in biodiesel. The smoke emission test revealed that B40 (biodiesel blend with 40% biodiesel and 60% diesel had the least black smoke compared to the conventional diesel. Diesel engine performance test indicated that the specific fuel consumption of biodiesel blend was increased sufficiently when the blending ratio was optimized. Thus, the reduction in exhaust emissions and reduction in brake-specific fuel consumption made the blends of caster seed oil (B20 a suitable alternative fuel for diesel and could help in controlling air pollution.

  8. Impact of an exploding LPG rail tank car onto a CASTOR spent fuel cask

    Energy Technology Data Exchange (ETDEWEB)

    Droste, B.; Probst, U.; Heller, W

    1999-07-01

    On 27 April 1999 a fire test was performed with a 45 m{sup 3} rail tank car partially filled with 10 m3 pressurised liquid propane. A CASTOR THTR/AVR spent fuel transport cask was positioned beside the propane tank as to suffer maximum damage from any explosion. About 17 min after fire ignition the propane tank ruptured. This resulted in a BLEVE with an expanding fireball, heat radiation, explosion overpressure, and tank fragments projected towards the cask. This imposed severe mechanical and thermal impacts directly onto the CASTOR cask, moving it 17 m from its original position. This involved rotation of the cask with the lid end travelling 10 m before it crashed into the ground. Post-test investigations of the CASTOR cask demonstrated that no loss of leaktightness or containment and shielding integrity occurred. (author)

  9. Castor oil biodiesel: an economic evaluation; Biodiesel de mamona: uma avaliacao economica

    Energy Technology Data Exchange (ETDEWEB)

    Pires, Monica de Moura; Alves, Jaenes Miranda; Almeida Neto, Jose Adolfo de; Almeida, Cezar Menezes; Sousa, Geovania Silva de; Cruz, Rosenira Serpa da; Monteiro, Renata; Lopes, Beatriz Sampaio; Robra, Sabine [Universidade Estadual de Santa Cruz, Ilheus, BA (Brazil). Grupo Bioenergia e Meio Ambiente]. E-mails: mpires@uesc.br; jaenes@uesc.br; jalmeida@uesc.br; roserpa@uesc.br

    2004-07-01

    The production cost of castor oil biodiesel by methyl way and its economic viability, using as reference the production cost data of castor oil and the implantation of the pilot plant at UESC - state university of Santa Cruz, Bahia State, Brazil was determined. From this information, it was seen that the estimated price of castor oil biodiesel is close to the diesel price in the Itabuna market, Bahia state, Brazil. The indicators show economic viability of the mini-power plant installation. Such information are preliminary estimative for the market and can be modified as function of changes in the main factors used to have the production costs, as well as the sectorial policies that drives the activity as much in levels of raw material production as in biodiesel.

  10. Castor oil for induction of labor in post-date pregnancies: A randomized controlled trial.

    Science.gov (United States)

    Gilad, Ronit; Hochner, Hagit; Savitsky, Bella; Porat, Shay; Hochner-Celnikier, Drorith

    2018-02-01

    Castor oil is a substance used for labor induction in an inpatient setting. However, its efficacy as an agent for the induction of labor, for post-date pregnancies in an outpatient setup is unknown. Efficacy of castor oil as an agent for the induction of labor, for post-date pregnancies in outpatient settings. Eighty-one women with a low-risk post-date singleton pregnancy with a Bishop score≤7, without effective uterine contractions were randomized to the intervention, 60ml of castor oil, or the control, 60ml of sun-flower oil. The primary outcome was proportion of women entering the active phase of labor 24, 36, 48h after ingestion. Secondary outcomes included meconium stained amniotic fluid, abnormal fetal heart rate tracing, cesarean section rate, instrumental deliveries, birth weight, 5min Apgar score, chorioamnionitis, hypertensive complications, retained placenta, and post-partum hemorrhage. Intervention and control groups included 38 and 43 women, respectively. No differences in baseline characteristics, except for age were noted. The observed interaction between castor oil and parity was significant (p interaction =0.02). Multiparous women in the intervention group exhibited a significant beneficial effect on entering active labor within 24, 36 and 48h after castor oil consumption compared with the placebo (Hazard Ratio=2.93, p=0.048; Hazard Ratio=3.29, p=0.026; Hazard Ratio=2.78, p=0.042 respectively). This effect was not noted among primiparous women. No differences in rate of obstetric complications or adverse neonatal outcomes were noted. Castor oil is effective for labor induction, in post-date multiparous women in outpatient settings. Copyright © 2017. Published by Elsevier Ltd.

  11. Reconstruction of the Cranial Vault Contour Using Tissue Expander and Castor Oil Prosthesis.

    Science.gov (United States)

    de Moraes, Sylvio Luiz Costa; Afonso, Alexandre Maurity de Paula; Santos, Roberto Gomes Dos; Mattos, Ricardo Pereira; Duarte, E Bruno Gomes

    2017-09-01

    Nowadays the reconstruction of craniofacial defects can be performed with different kinds of materials, which include the bone and the so-called biomaterials, which have the advantage of not needing a surgical site donor. Among these materials, great attention is given to polymers. In this large group, current attention is focused on the castor oil polymer, since this polymer is biocompatible, low cost, and has adequate strength for reconstruction of the craniomaxillofacial complex. This study aims to report the use of a prosthetic castor oil polymer for reconstruction of extensive defect, caused by a trauma, in the temporoparietal region.

  12. microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach.

    Science.gov (United States)

    Macchiaroli, Natalia; Cucher, Marcela; Zarowiecki, Magdalena; Maldonado, Lucas; Kamenetzky, Laura; Rosenzvit, Mara Cecilia

    2015-02-06

    microRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. The particular developmental and metabolic characteristics of cestode parasites highlight the importance of studying miRNA gene regulation in these organisms. Here, we perform a comprehensive analysis of miRNAs in the parasitic cestode Echinococcus canadensis G7, one of the causative agents of the neglected zoonotic disease cystic echinococcosis. Small RNA libraries from protoscoleces and cyst walls of E. canadensis G7 and protoscoleces of E. granulosus sensu stricto G1 were sequenced using Illumina technology. For miRNA prediction, miRDeep2 core algorithm was used. The output list of candidate precursors was manually curated to generate a high confidence set of miRNAs. Differential expression analysis of miRNAs between stages or species was estimated with DESeq. Expression levels of selected miRNAs were validated using poly-A RT-qPCR. In this study we used a high-throughput approach and found transcriptional evidence of 37 miRNAs thus expanding the miRNA repertoire of E. canadensis G7. Differential expression analysis showed highly regulated miRNAs between life cycle stages, suggesting a role in maintaining the features of each developmental stage or in the regulation of developmental timing. In this work we characterize conserved and novel Echinococcus miRNAs which represent 30 unique miRNA families. Here we confirmed the remarkable loss of conserved miRNA families in E. canadensis, reflecting their low morphological complexity and high adaptation to parasitism. We performed the first in-depth study profiling of small RNAs in the zoonotic parasite E. canadensis G7. We found that miRNAs are the preponderant small RNA silencing molecules, suggesting that these small RNAs could be an essential mechanism of gene regulation in this species. We also

  13. Production of bran castor biochar through slow pyrolysis

    Science.gov (United States)

    Pissinati de Rezende, E. I.; Mangrich, A. S.; Batista, M. G. F.; Toledo, J. M. S.; Novotny, E. H.

    2012-04-01

    Pyrolysis is a thermal process of great importance in the present context, since it constitutes a significant alternative to adequate use of organic waste. The principal products obtained in the pyrolysis of discarded biomass are bio-oil, biogas and biochar. Biochar, in turn, may play a relevant role when applied to the soil to sequester carbon and as a soil conditioner, a material comparable to organic matter of Indians Black Earths from the Amazon Region [1]. Seeking to determine the best methods of preparation of biochar, we studied the pyrolysis of bran castor residue of the Brazilian biodiesel industry. Eight samples, from FM1 to FM8, were prepared in a factorial design 23 using two temperature (300 and 350 °C), two heating velocity (5 and 10 °C min-1) and two period of heating (30 and 60 min). The eight samples were studied using the spectroscopy: EPR, FTIR, RMN, XPS, and elemental analysis. By elemental analysis, the samples that keep for lower temperature of pyrolysis, 300 °C, showed H/C and N/C ratios greater than the samples of 350 °C. That higher value can be attributed to chemical structure more aliphatic than aromatic mainly in the FM7 sample (V = 10 °C min-1, T = 300 °C, P = 30 min). The greater N/C ratio correlated with a superior amount of nitrogenous functions, presenting by both FM7 and FM4 samples, as determined by 13C NMR spectroscopy with absorptions in 175 ppm (amide) and 55 ppm (N-alkyl).

  14. The invasion and expansion of three North American species of goldenrod (Solidago canadensis L. sensu lato. S. gigantea Ait. and S. graminifolia (L Salisb in Poland

    Directory of Open Access Journals (Sweden)

    Małgorzata Guzikowa

    2014-01-01

    Full Text Available The geographical distributions of three adventive species of North American goldenrods (S. canadensis L. s.l., S. gigantea Ait. and S. graminifolin (L. Salisb. throughout Poland are presented. The history of escape, initial establishent and subsequent spread are documented on the basis of almost 1400 herbarium collections and site records of the authors. Of the three species S. gigantea is the most aggressive S. cunadensis has continued to spread vigorously. but S. graminifolia has barely moved beyond its initial area of establishment. The first two species occur essentially throughout Poland. having expanded from centers in southwestern Poland in the decade between 1840 to 1850. Both of these plants are spreading rapidly and producing vigorous populations which thrive in disturbed and semi-disturbed environments and S. gigantea seems to have now attained the capability of invading stabilized habitats and communities. They are in the process of becoming a serious threat to many natural environments and ecosystems in Poland.

  15. Vaccination of Elk (Cervus canadensis) with Brucella abortus Strain RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes Does Not Induce Adequate Protection against Experimental Brucella abortus Challenge.

    Science.gov (United States)

    Nol, Pauline; Olsen, Steven C; Rhyan, Jack C; Sriranganathan, Nammalwar; McCollum, Matthew P; Hennager, Steven G; Pavuk, Alana A; Sprino, Phillip J; Boyle, Stephen M; Berrier, Randall J; Salman, Mo D

    2016-01-01

    In recent years, elk (Cervus canadensis) have been implicated as the source of Brucella abortus infection for numerous cattle herds in the Greater Yellowstone Area. In the face of environmental and ecological changes on the landscape, the range of infected elk is expanding. Consequently, the development of effective disease management strategies for wild elk herds is of utmost importance, not only for the prevention of reintroduction of brucellosis to cattle, but also for the overall health of the Greater Yellowstone Area elk populations. In two studies, we evaluated the efficacy of B. abortus strain RB51 over-expressing superoxide dismutase and glycosyltransferase for protecting elk from infection and disease caused by B. abortus after experimental infection with a virulent B. abortus strain. Our data indicate that the recombinant vaccine does not protect elk against brucellosis. Further, work is needed for development of an effective brucellosis vaccine for use in elk.

  16. Quantitative proteomics of seed filling in castor: comparison with soybean and rapeseed reveals differences between photosynthetic and nonphotosynthetic seed metabolism.

    Science.gov (United States)

    Houston, Norma L; Hajduch, Martin; Thelen, Jay J

    2009-10-01

    Seed maturation or seed filling is a phase of development that plays a major role in the storage reserve composition of a seed. In many plant seeds photosynthesis plays a major role in this process, although oilseeds, such as castor (Ricinus communis), are capable of accumulating oil without the benefit of photophosphorylation to augment energy demands. To characterize seed filling in castor, a systematic quantitative proteomics study was performed. Two-dimensional gel electrophoresis was used to resolve and quantify Cy-dye-labeled proteins expressed at 2, 3, 4, 5, and 6 weeks after flowering in biological triplicate. Expression profiles for 660 protein spot groups were established, and of these, 522 proteins were confidently identified by liquid chromatography-tandem mass spectrometry by mining against the castor genome. Identified proteins were classified according to function, and the most abundant groups of proteins were involved in protein destination and storage (34%), energy (19%), and metabolism (15%). Carbon assimilatory pathways in castor were compared with previous studies of photosynthetic oilseeds, soybean (Glycine max) and rapeseed (Brassica napus). These comparisons revealed differences in abundance and number of protein isoforms at numerous steps in glycolysis. One such difference was the number of enolase isoforms and their sum abundance; castor had approximately six times as many isoforms as soy and rapeseed. Furthermore, Rubisco was 11-fold less prominent in castor compared to rapeseed. These and other differences suggest some aspects of carbon flow, carbon recapture, as well as ATP and NADPH production in castor differs from photosynthetic oilseeds.

  17. An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library

    Directory of Open Access Journals (Sweden)

    Wallis James G

    2007-07-01

    Full Text Available Abstract Background Castor seeds are a major source for ricinoleate, an important industrial raw material. Genomics studies of castor plant will provide critical information for understanding seed metabolism, for effectively engineering ricinoleate production in transgenic oilseeds, or for genetically improving castor plants by eliminating toxic and allergic proteins in seeds. Results Full-length cDNAs are useful resources in annotating genes and in providing functional analysis of genes and their products. We constructed a full-length cDNA library from developing castor endosperm, and obtained 4,720 ESTs from 5'-ends of the cDNA clones representing 1,908 unique sequences. The most abundant transcripts are genes encoding storage proteins, ricin, agglutinin and oleosins. Several other sequences are also very numerous, including two acidic triacylglycerol lipases, and the oleate hydroxylase (FAH12 gene that is responsible for ricinoleate biosynthesis. The role(s of the lipases in developing castor seeds are not clear, and co-expressing of a lipase and the FAH12 did not result in significant changes in hydroxy fatty acid accumulation in transgenic Arabidopsis seeds. Only one oleate desaturase (FAD2 gene was identified in our cDNA sequences. Sequence and functional analyses of the castor FAD2 were carried out since it had not been characterized previously. Overexpression of castor FAD2 in a FAH12-expressing Arabidopsis line resulted in decreased accumulation of hydroxy fatty acids in transgenic seeds. Conclusion Our results suggest that transcriptional regulation of FAD2 and FAH12 genes maybe one of the mechanisms that contribute to a high level of ricinoleate accumulation in castor endosperm. The full-length cDNA library will be used to search for additional genes that affect ricinoleate accumulation in seed oils. Our EST sequences will also be useful to annotate the castor genome, which whole sequence is being generated by shotgun sequencing at

  18. Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds

    International Nuclear Information System (INIS)

    Fernandes, K.V.; Deus-de-Oliveira, N.; Godoy, M.G.; Guimarães, Z.A.S.; Nascimento, V.V.; Melo, E.J.T. de; Freire, D.M.G.; Dansa-Petretski, M.; Machado, O.L.T.

    2012-01-01

    Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death was assessed by cell counting and measurement of lactate dehydrogenase activity. The limit of detection of the Vero cell assay was 10 ng/mL using a concentration of 1.6 × 10 5 cells/well. Solid-state fermentation (SSF) and treatment with calcium compounds were used as cake detoxification processes. For SSF, Aspergillus niger was grown using a castor cake as a substrate, and this cake was analyzed after 24, 48, 72, and 96 h of SSF. Ricin was eliminated after 24 h of SSF treatment. The cake was treated with 4 or 8% Ca(OH) 2 or CaO, and both the toxicity and the allergenic properties were entirely abolished. A by-product free of toxicity and allergens was obtained

  19. Red fox, Vulpes vulpes, kills a European beaver, Castor fiber, kit

    OpenAIRE

    Kile, Nils B.; Nakken, Petter J.; Rosell, Frank; Espeland, Sigurd

    1996-01-01

    We observed an adult Red Fox (Vulpes vulpes) attack, kill and partially consume a 2-month-old female kit European Beaver (Castor fiber) near its lodge in Norway. The inner organs were consumed first. One adult beaver apparently attempted to frighten the fox away by tail-slapping.

  20. Synthesis and properties of radiation modified thermally cured castor oil based polyurethanes

    Energy Technology Data Exchange (ETDEWEB)

    Mortley, Aba [Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, P.O. Box 17000, Stn Forces, Kingston, ON, K7K 7B4 (Canada)], E-mail: aba.mortley@rmc.ca; Bonin, H.W.; Bui, V.T. [Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, P.O. Box 17000, Stn Forces, Kingston, ON, K7K 7B4 (Canada)

    2007-12-15

    Thermally cured polyurethanes were prepared from castor oil and hexamethylene diisocyanate (HMDI). Due to the long aliphatic chain of the castor oil component of polyurethane, thermal curing of castor oil based polyurethane (COPU) is limited by increasing polymer viscosity. To enhance further crosslinking, COPUs were exposed to doses up to 3.0 MGy produced by the mixed ionizing radiation field of a SLOWPOKE-2 research nuclear reactor. The physico-mechanical properties of castor oil based polyurethanes (COPU), unirradiated and irradiated, were characterized by mechanical tensile tests. A four-fold increase in modulus and tensile strength values from 0.930 to 4.365 MPa and 0.149 to 0.747 MPa, respectively, suggests improved physico-mechanical properties resulting from radiation. The changing areas of the carbonyl and the NH absorbance peaks and the disappearance of the isocyanate peak in the FTIR spectra as radiation progressed, indicates increased hydrogen bonding and intermolecular crosslinking, which is in agreement with the mechanical tests. Unchanging {sup 13}C solid state NMR spectra imply limited sample degradation with increasing radiation.

  1. Castor diacylglycerol acyltransferase type1(DGAT1)displays greater activity with diricinolein than Arabidopsis DGAT1

    Science.gov (United States)

    Castor oil contains the hydroxy fatty acid ricinoleate as a major (90%) component. The diacylglycerol acyltransferase (DGAT) carries out the final reaction step in the biosynthesis of triacylglycerol, the principal constituent of seed oil, and has been considered to be the step that controls the oil...

  2. Recycling of waste poly(ethylene terephthalate) with castor oil using microwave heating

    Czech Academy of Sciences Publication Activity Database

    Beneš, Hynek; Slabá, J.; Walterová, Zuzana; Rais, David

    2013-01-01

    Roč. 98, č. 11 (2013), s. 2232-2243 ISSN 0141-3910 R&D Projects: GA MPO 2A-2TP1/135 Institutional support: RVO:61389013 Keywords : poly(ethylene terephthalate) * castor oil * chemical recycling Subject RIV: CD - Macromolecular Chemistry Impact factor: 2.633, year: 2013

  3. Life cycle assessment of castor-based biorefinery: a well to wheel LCA

    DEFF Research Database (Denmark)

    Khoshnevisan, Benyamin; Rafiee, Shahin; Tabatabaei, Meisam

    2017-01-01

    of their ability in converting biomass into a spectrum of marketable products and bioenergies. This study was aimed at developing different novel castor-based biorefinery scenarios for generating biodiesel and other co-products, i.e., ethanol and biogas. In these scenarios, glycerin, heat, and electricity were...

  4. Undecylenic acid: a valuable and physiologically active renewable building block from castor oil.

    Science.gov (United States)

    Van der Steen, Marijke; Stevens, Christian V

    2009-01-01

    A lot of attention is currently being paid to the transition to a biobased economy. In this movement, most efforts concentrate on the development of bioenergy applications including bioethanol, biodiesel, thermochemical conversion of biomass, and others. However, in the energy sector other nonbiomass alternatives are known, whereas no valuable alternatives are available when thinking about chemical building blocks. Therefore, it is also essential to develop new routes for the synthesis of bio-based chemicals and materials derived thereof. Such intermediates can originate either from plants or from animals. Castor oil is a non-edible oil extracted from the seeds of the castor bean plant Ricinus communis (Euphorbiaceae), which grows in tropical and subtropical areas. Globally, around one million tons of castor seeds are produced every year, the leading producing areas being India, PR China, and Brazil.2 10-Undecenoic acid or undecylenic acid is a fatty acid derived from castor oil that, owing to its bifunctional nature, has many possibilities to develop sustainable applications.

  5. Factors affecting scent-marking behaviour in Eurasion beaver (Castor fiber)

    NARCIS (Netherlands)

    Rosell, F.; Nolet, B.A.

    1997-01-01

    We tested the hypothesis that a main function of territory marking in Eurasian beaver (Castor fiber) is defense of the territory. The results showed that: (1) beaver colonies with close neighbors scent-mark more often than isolated ones; (2) the number of scent markings increased significantly with

  6. Salinity and cationic nature of irrigation water on castor bean cultivation

    Directory of Open Access Journals (Sweden)

    Geovani S. de Lima

    Full Text Available ABSTRACT This study aimed to evaluate the water relations, cell damage percentage and growth of the castor bean cv. ‘BRS Energia’ as a function of salinity and cationic nature of the water used in irrigation. The experiment was conducted in drainage lysimeters under greenhouse conditions in eutrophic Grey Argisol of sandy loam texture. Six combinations of water salinity and cations were studied (S1 - Control; S2 - Na+, S3 - Ca2+, S4 - Na+ + Ca2+; S5 - K+ and S6 - Na+ + Ca2+ + Mg2+, in a randomized block design with four replicates. In the control (S1, plants were irrigated with 0.6 dS m-1 water, whereas the other treatments received 4.5 dS m-1 water, obtained by adding different salts, all in the chloride form. Higher relative water content in the leaf blade of plants irrigated with K+-salinized water associated with leaf succulence are indicative of tolerance of the castor bean cv. ‘BRS Energia’ to salinity. Saline stress negatively affected castor bean growth, regardless of cationic nature of water. Among the ions studied, ‘BRS Energia’ castor bean was more sensitive to the presence of sodium in the irrigation water, in terms of both water relations and leaf succulence.

  7. Pyrolysis kinetics and thermodynamic parameters of castor (Ricinus communis) residue using thermogravimetric analysis.

    Science.gov (United States)

    Kaur, Ravneet; Gera, Poonam; Jha, Mithilesh Kumar; Bhaskar, Thallada

    2018-02-01

    Castor plant is a fast-growing, perennial shrub from Euphorbiaceae family. More than 50% of the residue is generated from its stems and leaves. The main aim of this work is to study the pyrolytic characteristics, kinetics and thermodynamic properties of castor residue. The TGA experiments were carried out from room temperature to 900 °C under an inert atmosphere at different heating rates of 5, 10, 15, 20, 30 and 40 °C/min. The kinetic analysis was carried using different models namely Kissinger, Flynn-Wall-Ozawa (FWO) and Kissinger-Akahira-Sunose (KAS). The average E ɑ calculated by FWO and KAS methods were 167.10 and 165.86 kJ/mole respectively. Gibbs free energy varied from 150.62-154.33 to 150.59-154.65 kJ/mol for FWO and KAS respectively. The HHV of castor residue was 14.43 MJ/kg, considered as potential feedstock for bio-energy production. Kinetic and thermodynamic results will be useful input for the design of pyrolytic process using castor residue as feedstock. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Organic matter and soil water content influence on BRS 188 castor bean growth

    Energy Technology Data Exchange (ETDEWEB)

    Lacerda, Rogerio Dantas de; Araujo, Ester Luiz de; Nascimento, Elka Costa Santos; Barros Junior, Genival [Universidade Federal de Campina Grande (UFCG), PB (Brazil); Guerra, Hugo O. Carvallo; Chaves, Lucia Helena G. [Universidade Federal de Campina Grande (UAEAg/UFCG), PB (Brazil). Unidade Academica de Engenharia Agricola

    2008-07-01

    The castor bean culture has been highlighted due to the several applications of its oil, which constitutes one of the best row materials for biodiesel manufacturing, and the base for several other industrial products. The objective of the present work was to study the effect of different soil water and soil organic matter on the castor bean growth. The experiment was conducted from April to August 2006 under greenhouse conditions using a randomized block 2x4 factorial design with two soil organic mater content (5.0 g.kg{sup -1} e 25.0 g.kg{sup -1}), four levels of available water (100, 90, 80 e 70% ) and three replicates. For this, 24 plastic containers, 75 kg capacity, were used on which was grown one plant 120 days after the seedling. At regular intervals the plant height was measured and the results analyzed statistically. For the qualitative treatments (with and without organic matter) the treatment means were compared through the Tukey test. For the quantitative ones (water levels) were used regressions. The castor bean cultivar height was significantly influenced by the organic matter content only after 80 days. Castor bean height increased significantly with the soil water content after 40 days of growing. (author)

  9. Thermal Stability and Lubrication Properties of Biodegradable Castor Oil on AISI 4140 Steel

    Directory of Open Access Journals (Sweden)

    María Teresa Hernández-Sierra

    2018-06-01

    Full Text Available Lubricants have much importance in several industries, principally serving to reduce friction and wear in mechanical elements. In this study, the influence of Castor oil as bio-lubricant on the friction and wear performance of AISI 4140 steel was investigated. For that purpose, pin-on-disk friction tests were conducted according to ASTM G-99, by using pins of tungsten carbide (WC as counterparts. The experiments were performed at two different temperatures. This work also presents the Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR and 1H-NMR analyses for the chemical characterization of oils and a study of their degradation by oxidation. The analysis of the damage caused to steel due to friction was deepened by analyzing its microhardness and microstructure in the worn zone. As a reference, the same experiments were performed with a commercial oil. The friction behavior of 4140 steel/WC with Castor oil lubrication at the two temperatures was notably better than that obtained under the reference oil lubrication. The kinetic friction coefficient (µk was up to 76% lower. However, a slight increase in steel wear was observed under Castor oil lubrication. Despite this, these results suggest that Castor oil could be used as bio-lubricant in systems which are susceptible to levels of high friction.

  10. Differential Contribution of Malic Enzymes during Soybean and Castor Seeds Maturation.

    Directory of Open Access Journals (Sweden)

    Mariel Claudia Gerrard Wheeler

    Full Text Available Malic enzymes (ME catalyze the decarboxylation of malate generating pyruvate, CO2 and NADH or NADPH. In some organisms it has been established that ME is involved in lipids biosynthesis supplying carbon skeletons and reducing power. In this work we studied the MEs of soybean and castor, metabolically different oilseeds. The comparison of enzymatic activities, transcript profiles and organic acid contents suggest different metabolic strategies operating in soybean embryo and castor endosperm in order to generate precursors for lipid biosynthesis. In castor, the malate accumulation pattern agrees with a central role of this metabolite in the provision of carbon to plastids, where the biosynthesis of fatty acids occurs. In this regard, the genome of castor possesses a single gene encoding a putative plastidic NADP-ME, whose expression level is high when lipid deposition is active. On the other hand, NAD-ME showed an important contribution to the maturation of soybean embryos, perhaps driving the carbon relocation from mitochondria to plastids to support the fatty acids synthesis in the last stages of seed filling. These findings provide new insights into intermediary metabolism in oilseeds and provide new biotechnological targets to improve oil yields.

  11. Lipase catalyzed transesterification of castor oil by straight chain higher alcohols.

    Science.gov (United States)

    Malhotra, Deepika; Mukherjee, Joyeeta; Gupta, Munishwar N

    2015-03-01

    Biolubricants from Castor oil were produced enzymatically by transesterification with higher alcohols using a lipase mixture of immobilized Mucor miehei (RMIM) and immobilized Candida antarctica lipase B (Novozym 435) under low water conditions. The conversions were in the range of 80-95% under the optimized conditions. Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  12. Derivatization of castor oil based estolide esters: Preparation of epoxides and cyclic carbonates

    Science.gov (United States)

    Estolides that are based on castor oil and oleic acid are versatile starting points for the production of industrial fluids with new properties. A variety of unsaturated estolides were derivatized by epoxidation with hydrogen peroxide. The epoxidized estolides were further modified using supercritic...

  13. Evaluation of castor oil-based polyurethane membranes in rat bone-marrow cell culture.

    Science.gov (United States)

    Cerejo, Sofia de Amorim; Rahal, Sheila Canevese; Lima Neto, João Ferreira de; Voorwald, Fabiana Azevedo; Alvarenga, Fernanda da Cruz Landim e

    2011-10-01

    To evaluate three methods to isolate rats MSCs and to analyze the potential of a castor oil polyurethane base membrane as a scaffold for MSCs. Four male Wistar rats, aged 20-30 days were used. Bone marrow aspirates from femur and tibia were harvested using DMEM high glucose and heparin. The cell culture was performed in three different ways: direct culture and two types of density gradients. After 15 days, was made the 1st passage and analyzed cell viability with markers Hoerscht 33342 and propidium iodide. The MSCs were characterized by surface markers with the aid of flow cytometry. After this, three types of castor oil polyurethane membranes associated with the MSCs were kept on the 6-well plate for 5 days and were analyzed by optical microscopy to confirm cell aggregation and growth. Separation procedures 1 and 2 allowed adequate isolation of MSCs and favored cell growth with the passage being carried out at 70% confluence after 15 days in culture. The cells could not be isolated using procedure 3. When the 3 castor oil polyurethane membrane types were compared it was possible to observe that the growth of MSCs was around 80% in membrane type 3, 20% in type 2, and 10% in type 1. Both Ficoll-Hypaque densities allow isolation of rat MSCs, and especially castor oil-based membrane type 3 may be used as a scaffold for MSCs.

  14. Effect of Aqueous Extract of Acacia nilotica on Microbial and Castor ...

    African Journals Online (AJOL)

    Five (5) Albino rats of both sexes weighing between (85-125g) were used for each experiment and diarrhoea was induced using Castor oil. The extract showed remarkable anti-diarrhoea activity which was dose dependent at 100, 200 and 400mg evidenced by decrease in volume of intestinal content significantly different at ...

  15. Amelioration of Anti-Nutritive Effects of Castor Oil Seed ( Ricinus ...

    African Journals Online (AJOL)

    Three hundred and twenty (320) day old male broilers were used to investigate the amelioration of anti-nutritive effects of castor oil seed (Ricinus communis) meal in broilers' ration using natural fermentation and DL-Methionine supplementation. The experimental designed was a 4 × 2 factorial arrangement of dietary ...

  16. Synthesis and properties of radiation modified thermally cured castor oil based polyurethanes

    International Nuclear Information System (INIS)

    Mortley, Aba; Bonin, H.W.; Bui, V.T.

    2007-01-01

    Thermally cured polyurethanes were prepared from castor oil and hexamethylene diisocyanate (HMDI). Due to the long aliphatic chain of the castor oil component of polyurethane, thermal curing of castor oil based polyurethane (COPU) is limited by increasing polymer viscosity. To enhance further crosslinking, COPUs were exposed to doses up to 3.0 MGy produced by the mixed ionizing radiation field of a SLOWPOKE-2 research nuclear reactor. The physico-mechanical properties of castor oil based polyurethanes (COPU), unirradiated and irradiated, were characterized by mechanical tensile tests. A four-fold increase in modulus and tensile strength values from 0.930 to 4.365 MPa and 0.149 to 0.747 MPa, respectively, suggests improved physico-mechanical properties resulting from radiation. The changing areas of the carbonyl and the NH absorbance peaks and the disappearance of the isocyanate peak in the FTIR spectra as radiation progressed, indicates increased hydrogen bonding and intermolecular crosslinking, which is in agreement with the mechanical tests. Unchanging 13 C solid state NMR spectra imply limited sample degradation with increasing radiation

  17. Film-forming properties of castor oil polyol ester blends in elastohydrodynamic conditions

    Science.gov (United States)

    The viscosities and elastohydrodynamic (EHD) film thickness properties of binary blends of castor oil with polyol esters were determined experimentally. Predicted blend viscosity was calculated from the viscosity of the pure blend components. Measured viscosity values were closer to the values pre...

  18. Experimental investigation on performance and exhaust emissions of castor oil biodiesel from a diesel engine.

    Science.gov (United States)

    Shojaeefard, M H; Etgahni, M M; Meisami, F; Barari, A

    2013-01-01

    Biodiesel, produced from plant and animal oils, is an important alternative to fossil fuels because, apart from dwindling supply, the latter are a major source of air pollution. In this investigation, effects of castor oil biodiesel blends have been examined on diesel engine performance and emissions. After producing castor methyl ester by the transesterification method and measuring its characteristics, the experiments were performed on a four cylinder, turbocharged, direct injection, diesel engine. Engine performance (power, torque, brake specific fuel consumption and thermal efficiency) and exhaust emissions were analysed at various engine speeds. All the tests were done under 75% full load. Furthermore, the volumetric blending ratios of biodiesel with conventional diesel fuel were set at 5, 10, 15, 20 and 30%. The results indicate that lower blends of biodiesel provide acceptable engine performance and even improve it. Meanwhile, exhaust emissions are much decreased. Finally, a 15% blend of castor oil-biodiesel was picked as the optimized blend of biodiesel-diesel. It was found that lower blends of castor biodiesel are an acceptable fuel alternative for the engine.

  19. Prolonged local anesthetic action through slow release from poly (lactic acid co castor oil).

    Science.gov (United States)

    Sokolsky-Papkov, Marina; Golovanevski, Ludmila; Domb, Abraham J; Weiniger, Carolyn F

    2009-01-01

    To evaluate a new formulation of bupivacaine loaded in an injectable fatty acid based biodegradable polymer poly(lactic acid co castor oil) in prolonging motor and sensory block when injected locally. The polyesters were synthesized from DL: -lactic acid and castor oil with feed ratio of 4:6 and 3:7 w/w. Bupivacaine was dispersed in poly(fatty ester) liquid and tested for drug release in vitro. The polymer p(DLLA:CO) 3:7 loaded with 10% bupivacaine was injected through a 22G needle close to the sciatic nerve of ICR mice and the duration of sensory and motor nerve blockade was measured. The DL: -lactic acid co castor oil p(DLLA:CO) 3:7 released 65% of the incorporated bupivacaine during 1 week in vitro. Single injection of 10% bupivacaine loaded into this polymer caused motor block that lasted 24 h and sensory block that lasted 48 h. Previously we developed a ricinoleic acid based polymer with incorporated bupivacaine which prolonged anesthesia to 30 h. The new polymer poly(lactic acid co castor oil) 3:7 provides slow release of effective doses of the incorporated local anesthetic agent and prolongs anesthesia to 48 h.

  20. Antimicrobial action of sodium hypochlorite and castor oil solutions for denture cleaning - in vitro evaluation.

    Science.gov (United States)

    Salles, Marcela Moreira; Oliveira, Viviane de Cássia; Souza, Raphael Freitas; Silva, Cláudia Helena Lovato; Paranhos, Helena de Freitas Oliveira

    2015-01-01

    The objective of this in vitro study was to evaluate the antimicrobial action of sodium hypochlorite (0.25% and 0.50%) and 10% castor oil solutions against specific microorganisms, by counting Colony Forming Units (CFU) of clinically important bacteria and Candida species. Acrylic resin specimens (n = 320; Lucitone 550) were obtained from square metal matrices (10 x 10 x 2 mm), sterilized by microwave (650W, for 6 minutes) and contaminated by Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Bacillus subtilis, Escherichia coli, Streptococcus mutans, Enterococcus faecalis and Candida glabrata. The specimens were immersed for 20 minutes in one of the following hygiene solutions (n = 10/each): A - 0.25% Sodium hypochlorite; B - 0.5% Sodium hypochlorite; C - 10% Castor oil solution; and D (Control) - saline. Adhered cells were suspended and inoculated into a selective solid medium (37ºC for 24 h). The Student's t-test (α = 0.05) was performed to compare log10(CFU+1)/mL between Groups C and D. The results showed that sodium hypochlorite (0.25% and 0.5%) completely eliminated all detectable microorganisms. The castor oil solution eliminated B. subtilis and reduced counts for other strains. Differences between C and D were significant (p castor oil solution provided moderate efficacy and performed differently on the tested species, with the strongest effect on B. subtilis and with non-significant action on E. faecalis.

  1. A rapid method for evaluation of the oxidation stability of castor oil FAME: influence of antioxidant type and concentration

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Susana V.; Luna, F. Murilo T.; Rola, Estelio M. Jr.; Azevedo, Diana C.S.; Cavalcante, Celio L. Jr. [Universidade Federal do Ceara, Departamento de Engenharia Quimica, Grupo de Pesquisa em Separacoes por Adsorcao - GPSA, Campus do Pici, 709, Fortaleza, CE, 60.455-900 (Brazil)

    2009-10-15

    The oxidation stability of castor oil fatty methyl ester (FAME), doped with four different phenolic antioxidants, was evaluated using a rapid method of thermal and air-contact degradation. The methodology is based on the induction times observed when the samples are contacted with pure oxygen at elevated pressures and temperatures. The results indicate different performances of the antioxidants as well as synergisms between antioxidants and biodiesel. In general, the addition of antioxidants increased from 6-15 times the stability of castor oil FAME., with BHA (butylated hydroxyanisol) showing the best results for improving antioxidation in castor oil biodiesel. (author)

  2. Microdistribution of {sup 241}Am in structures of submerged macrophyte Elodea canadensis growing in the Yenisei River

    Energy Technology Data Exchange (ETDEWEB)

    Bondareva, L., E-mail: lydiabondareva@gmail.co [Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk 660036 (Russian Federation); Vlasova, I. [Chemistry Department Lomonosov Moscow State University, Leninskie Gory, Moscow 119991 (Russian Federation); Mogilnaya, O.; Bolsunovsky, A. [Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk 660036 (Russian Federation); Kalmykov, S. [Chemistry Department Lomonosov Moscow State University, Leninskie Gory, Moscow 119991 (Russian Federation)

    2010-01-15

    A submerged macrophyte of the Yenisei River, Elodea canadensis, was used to study the microdistribution of the artificial radionuclide {sup 241}Am among different components of the plant. The total amount of {sup 241}Am added to the experimental system was 1850 +- 31 Bq/L. The total amount of {sup 241}Am accumulated by the plants was 182 Bq per sample, or 758,333 +- 385 Bq/kg dry mass. It has been found that the major portion of {sup 241}Am accumulated by E. canadensis, up to 85%, was bound to solid components of the cells. It is observed that the microdistribution of {sup 241}Am within different components of the submerged plant E. canadensis was not uniform. {sup 241}Am distribution vary depending on the age of the leaf blades, the state of the cells and morphological features of the plant stem.

  3. Extracellular polymer substance synthesized by a halophilic bacterium Chromohalobacter canadensis 28.

    Science.gov (United States)

    Radchenkova, Nadja; Boyadzhieva, Ivanka; Atanasova, Nikolina; Poli, Annarita; Finore, Ilaria; Di Donato, Paola; Nicolaus, Barbara; Panchev, Ivan; Kuncheva, Margarita; Kambourova, Margarita

    2018-04-03

    Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration. Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer. The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry. After optimization of the culture medium and physicochemical conditions for cultivation, polymer biosynthesis increased more than 2-fold. The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v). Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w). HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w). EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9. The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability. This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction. The synthesized biopolymer shows an extremely high hydrophilicity, due to the simultaneous presence of PGA and EPS. The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.

  4. Cost-benefit analysis for invasive species control: the case of greater Canada goose Branta canadensis in Flanders (northern Belgium

    Directory of Open Access Journals (Sweden)

    Nikolaas Reyns

    2018-01-01

    Full Text Available Background Sound decisions on control actions for established invasive alien species (IAS require information on ecological as well as socio-economic impact of the species and of its management. Cost-benefit analysis provides part of this information, yet has received relatively little attention in the scientific literature on IAS. Methods We apply a bio-economic model in a cost-benefit analysis framework to greater Canada goose Branta canadensis, an IAS with documented social, economic and ecological impacts in Flanders (northern Belgium. We compared a business as usual (BAU scenario which involved non-coordinated hunting and egg destruction with an enhanced scenario based on a continuation of these activities but supplemented with coordinated capture of moulting birds. To assess population growth under the BAU scenario we fitted a logistic growth model to the observed pre-moult capture population. Projected damage costs included water eutrophication and damage to cultivated grasslands and were calculated for all scenarios. Management costs of the moult captures were based on a representative average of the actual cost of planning and executing moult captures. Results Comparing the scenarios with different capture rates, different costs for eutrophication and various discount rates, showed avoided damage costs were in the range of 21.15 M€ to 45.82 M€ under the moult capture scenario. The lowest value for the avoided costs applied to the scenario where we lowered the capture rate by 10%. The highest value occurred in the scenario where we lowered the real discount rate from 4% to 2.5%. Discussion The reduction in damage costs always outweighed the additional management costs of moult captures. Therefore, additional coordinated moult captures could be applied to limit the negative economic impact of greater Canada goose at a regional scale. We further discuss the strengths and weaknesses of our approach and its potential application to other

  5. Cost-benefit analysis for invasive species control: the case of greater Canada goose Branta canadensis in Flanders (northern Belgium)

    Science.gov (United States)

    Casaer, Jim; De Smet, Lieven; Devos, Koen; Huysentruyt, Frank; Robertson, Peter A.; Verbeke, Tom

    2018-01-01

    Background Sound decisions on control actions for established invasive alien species (IAS) require information on ecological as well as socio-economic impact of the species and of its management. Cost-benefit analysis provides part of this information, yet has received relatively little attention in the scientific literature on IAS. Methods We apply a bio-economic model in a cost-benefit analysis framework to greater Canada goose Branta canadensis, an IAS with documented social, economic and ecological impacts in Flanders (northern Belgium). We compared a business as usual (BAU) scenario which involved non-coordinated hunting and egg destruction with an enhanced scenario based on a continuation of these activities but supplemented with coordinated capture of moulting birds. To assess population growth under the BAU scenario we fitted a logistic growth model to the observed pre-moult capture population. Projected damage costs included water eutrophication and damage to cultivated grasslands and were calculated for all scenarios. Management costs of the moult captures were based on a representative average of the actual cost of planning and executing moult captures. Results Comparing the scenarios with different capture rates, different costs for eutrophication and various discount rates, showed avoided damage costs were in the range of 21.15 M€ to 45.82 M€ under the moult capture scenario. The lowest value for the avoided costs applied to the scenario where we lowered the capture rate by 10%. The highest value occurred in the scenario where we lowered the real discount rate from 4% to 2.5%. Discussion The reduction in damage costs always outweighed the additional management costs of moult captures. Therefore, additional coordinated moult captures could be applied to limit the negative economic impact of greater Canada goose at a regional scale. We further discuss the strengths and weaknesses of our approach and its potential application to other IAS. PMID

  6. Chemical composition and antimicrobial activity of solidago canadensis linn. Root essential oil.

    Science.gov (United States)

    Mishra, Devendra; Joshi, Shivani; Bisht, Ganga; Pilkhwal, Sangeeta

    2010-06-01

    The essential oil from the roots of Solidago canadensis Linn. (fam. Asteraceae) was analyzed by GC, GC/MS and NMR spectroscopy. Thirty nine constituents comprising 75.4% of the total oil were identified from the oil. Thymol constituted 20.25% of the oil followed by α-copaene (6.26%) and carvacrol (5.51%). The antimicrobial activity of the oil was evaluated using disc diffusion method. Results showed that the oil exhibited significant antibacterial activity against S. feacalis and E. coli whereas it showed moderate antifungal activity against C. albicans.

  7. CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF SOLIDAGO CANADENSIS LINN. ROOT ESSENTIAL OIL

    OpenAIRE

    Mishra, Devendra; Joshi, Shivani; Bisht, Ganga; Pilkhwal, Sangeeta

    2010-01-01

    The essential oil from the roots of Solidago canadensis Linn. (fam. Asteraceae) was analyzed by GC, GC/MS and NMR spectroscopy. Thirty nine constituents comprising 75.4% of the total oil were identified from the oil. Thymol constituted 20.25% of the oil followed by α-copaene (6.26%) and carvacrol (5.51%). The antimicrobial activity of the oil was evaluated using disc diffusion method. Results showed that the oil exhibited significant antibacterial activity against S. feacalis and E. coli wher...

  8. Kunnskapsgrunnlag for handlingsplan mot vasspest (Elodea canadensis) og smal vasspest (Elodea nuttallii) i Norge

    OpenAIRE

    Mjelde, M.; Berge, D.; Edvardsen, H.

    2012-01-01

    Den foreliggende rapporten utgjør kunnskapsgrunnlaget for Handlingsplan mot vasspest (Elodea canadensis) og smal vasspest (Elodea nuttallii). Rapporten gir en oppdatert oversikt over de to artenes biologi og økologi, utbredelse og sprednings­veier, samt kjente effekter på vannøkosystemet. Kunnskapsbehov diskuteres, bl.a. knyttet til artenes økologiske krav og effekter på biologisk mangfold. Rapporten omtaler tiltak for å hindre videre spredning og tiltak for å redusere eller bekjempe bestande...

  9. Vasspest (Elodea canadensis) og smal vasspest (Elodea nuttallii) Jæren 2006

    OpenAIRE

    Mjelde, M.

    2006-01-01

    Årsliste 2006 For å skaffe en oversikt over status for vasspest (Elodea canadensis) i Skas-Heigre-kanalen og nærliggende lokaliteter ble vannvegetasjonen i området kartlagt i 2006. Vasspest har fortsatt stor forekomst i Skas-Heigre-kanalen ved Soma, der den ble registrert første gang i 2005. Den ble ikke registrert lenger ned i kanalen, heller ikke i Grudavatn. Heller ikke i nærliggende lokaliteter ble vasspest registrert. Lokaliteten for smal vasspest (Elodea nuttallii), Fuglestadåna, ble...

  10. Biomolecular characterization and protein sequences of the Campanian hadrosaur B. canadensis.

    Science.gov (United States)

    Schweitzer, Mary H; Zheng, Wenxia; Organ, Chris L; Avci, Recep; Suo, Zhiyong; Freimark, Lisa M; Lebleu, Valerie S; Duncan, Michael B; Vander Heiden, Matthew G; Neveu, John M; Lane, William S; Cottrell, John S; Horner, John R; Cantley, Lewis C; Kalluri, Raghu; Asara, John M

    2009-05-01

    Molecular preservation in non-avian dinosaurs is controversial. We present multiple lines of evidence that endogenous proteinaceous material is preserved in bone fragments and soft tissues from an 80-million-year-old Campanian hadrosaur, Brachylophosaurus canadensis [Museum of the Rockies (MOR) 2598]. Microstructural and immunological data are consistent with preservation of multiple bone matrix and vessel proteins, and phylogenetic analyses of Brachylophosaurus collagen sequenced by mass spectrometry robustly support the bird-dinosaur clade, consistent with an endogenous source for these collagen peptides. These data complement earlier results from Tyrannosaurus rex (MOR 1125) and confirm that molecular preservation in Cretaceous dinosaurs is not a unique event.

  11. Solidago canadensis invasion affects soil N-fixing bacterial communities in heterogeneous landscapes in urban ecosystems in East China.

    Science.gov (United States)

    Wang, Congyan; Jiang, Kun; Zhou, Jiawei; Wu, Bingde

    2018-03-12

    Soil nitrogen-fixing bacterial communities (SNB) can increase the level of available soil N via biological N-fixation to facilitate successful invasion of several invasive plant species (IPS). Meanwhile, landscape heterogeneity can greatly enhance regional invasibility and increase the chances of successful invasion of IPS. Thus, it is important to understand the soil micro-ecological mechanisms driving the successful invasion of IPS in heterogeneous landscapes. This study performed cross-site comparisons, via metagenomics, to comprehensively analyze the effects of Solidago canadensis invasion on SNB in heterogeneous landscapes in urban ecosystems. Rhizospheric soil samples of S. canadensis were obtained from nine urban ecosystems [Three replicate quadrats (including uninvaded sites and invaded sites) for each type of urban ecosystem]. S. canadensis invasion did not significantly affect soil physicochemical properties, the taxonomic diversity of plant communities, or the diversity and richness of SNB. However, some SNB taxa (i.e., f_Micromonosporaceae, f_Oscillatoriaceae, and f_Bacillaceae) changed significantly with S. canadensis invasion. Thus, S. canadensis invasion may alter the community structure, rather than the diversity and richness of SNB, to facilitate its invasion process. Of the nine urban ecosystems, the diversity and richness of SNB was highest in farmland wasteland. Accordingly, the community invasibility of farmland wasteland may be higher than that of the other types of urban ecosystem. In brief, landscape heterogeneity, rather than S. canadensis invasion, was the strongest controlling factor for the diversity and richness of SNB. One possible reason may be the differences in soil electrical conductivity and the taxonomic diversity of plant communities in the nine urban ecosystems, which can cause notable shifts in the diversity and richness of SNB. Copyright © 2018 Elsevier B.V. All rights reserved.

  12. The century storage facility. Why we have to give thoughts to the castor halls; Die Jahrhundert-Lager. Warum wir uns dringend Gedanken ueber die Castor-Hallen machen muessen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2017-01-15

    The authors discuss the situation of the German interim storage facilities for high-level nuclear waste. The Castor casks are supposed to be licensed as storage casks for 40 years. It is already foreseeable that after 40 years a final repository will not be available. This means that the Castor casks and the interim storage halls will have to be operated at least for the 21st century without valid licensing.

  13. First study about the development of adult Echinococcus canadensis G6 genotype of goat origin in experimentally infected dogs.

    Science.gov (United States)

    Soriano, Silvia Viviana; Debiaggi, María Florencia; Pierangeli, Nora Beatriz; Pianciola, Luis Alfredo; Bergagna, Héctor Fabián Jesús; Lazzarini, Lorena Evelina; Mazzeo, Melina Leonor; Basualdo, Juan Angel

    2016-09-15

    Echinococcus granulosus sensu lato (E. granulosus sl) must be considered as a species complex, comprising Echinococcus granulosus sensu stricto (E. granulosus ss, genotypes G1-G3), Echinococcus equinus (G4), Echinococcus ortleppi (G5) and Echinococcus canadensis (G6-G10) although the species status of E. canadensis is still controversial. These genotypes closely match the intermediate hosts associated strains described in earlier times among which E. canadensis G6 corresponds to the camel strain. As there are no studies concerning the development of adult stages of the G6 genotype from non-camel origin, the aims of the present study were: to characterize for the first time the development of E. canadensis G6 in dogs experimentally infected with protoscoleces derived from goats, to describe the resultant adult morphology, to evaluate the growth of their rostellar hooks from larval to adult stages and to determine the prepatent period of the strobilar stage of E. canadensis G6 derived from goats. The development of the strobilar stage of E. canadensis G6 genotype of goat origin was examined by studying the growth (variation of the total worm length) and segmentation in experimentally infected dogs at 14, 25, 35 and 56days post infection. A morphological characterization of 35-day-old worms as well as of larval and adult rostellar hooks was also carried out by conventional optical microscopic observations and/or by scanning electron microscopy. The prepatent period of the strobilar stage was assessed by microscopic examination of faeces from 2 infected dogs. Our results were compared with published data from the camel and other strains. The roles of the host, genotype and species in morphological and developmental features as well as the taxonomic position of E. canadensis G6 were discussed. The prepatent period of E. canadensis G6 genotype of goat origin was determined as at least, 41days. The obtained results contribute to increase the knowledge about the biology

  14. Intercropping for Management of Insect Pests of Castor, Ricinus communis, in the Semi—Arid Tropics of India

    Science.gov (United States)

    Srinivasa Rao, M.; Venkateswarlu, B.

    2012-01-01

    Intercropping is one of the important cultural practices in pest management and is based on the principle of reducing insect pests by increasing the diversity of an ecosystem. On—farm experiments were conducted in villages of semi—arid tropical (SAT) India to identify the appropriate combination of castor (Ricinus communis L.) (Malpighiales: Euphorbiaceae) and intercropping in relation to pest incidence. The diversity created by introducing cluster bean, cowpea, black gram, or groundnut as intercrops in castor (1:2 ratio proportions) resulted in reduction of incidence of insect pests, namely semilooper (Achaea janata L.), leaf hopper (Empoasca flavescens Fabricius), and shoot and capsule borer (Conogethes punctiferalis Guenee). A buildup of natural enemies (Microplitis, coccinellids, and spiders) of the major pests of castor was also observed in these intercropping systems and resulted in the reduction of insect pests. Further, these systems were more efficient agronomically and economically, and were thus more profitable than a castor monocrop. PMID:22934569

  15. The effect of herbivory on temporal and spatial dynamics of foliar nectar production in cotton and castor

    NARCIS (Netherlands)

    Wäckers, F.L.; Zuber, D.; Wunderlin, R.; Keller, F.

    2001-01-01

    The effects of feeding Spodoptera a littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae on the quantity and distribution of extrafloral nectar production by leaves of castor ((Ricinus communis) and cotton (Gossypium herbaceum) were investigated. Following larval feeding, the total volume of nectar

  16. The effect of herbivory on temporal and spatial dynamics of foliar nectar production in cotton and castor

    NARCIS (Netherlands)

    Wäckers, F.L.; Zuber, D.; Wunderlin, R.; Keller, F.

    2001-01-01

    The effects of feeding Spodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae) larvae on the quantity and distribution of extrafloral nectar production by leaves of castor (Ricinus communis) and cotton (Gossypium herbaceum) were investigated. Following larval feeding, the total volume of nectar

  17. Transesterification of triacetin and castor oil with methanol catalyzed by supported polyaniline-sulfate. A role of polymer morphology

    Czech Academy of Sciences Publication Activity Database

    Drelinkiewicz, A.; Kalemba-Jaje, Z.; Lalik, E.; Zieba, A.; Mucha, D.; Konyushenko, Elena; Stejskal, Jaroslav

    2013-01-01

    Roč. 455, 30 March (2013), s. 92-106 ISSN 0926-860X Institutional support: RVO:61389013 Keywords : transesterification * triacetin * castor oil Subject RIV: CD - Macromolecular Chemistry Impact factor: 3.674, year: 2013

  18. Analysis of the gamma radiation effects in the composite of polyurethane derived from castor oil and natural fibers

    International Nuclear Information System (INIS)

    Kienen, Victor D.; Todt, Matheus L.; Capellari, Giovanni S.; Azevedo, Elaine C.; Neto, Salvador C.

    2015-01-01

    Composite of Polyurethane derived from castor oil and natural fibers are obtained from renewable raw material, low cost, and for not assaulting nature. This paper analyzes the effects of gamma radiation on composite polyurethane derived from castor with sawdust irradiated with gamma radiation of 25 kGy . It was held from 3 tips bending tests and micrograph by scanning electron microscopy. The results indicate that gamma radiation decreases the breakdown voltage and the micrograph of the fracture indicates brittle fracture occurred. (author)

  19. Impact of the keystone species, the Eurasian beaver (Castor fiber), on habitat structure and its significance to mammals

    OpenAIRE

    Samas, Arūnas

    2016-01-01

    Eurasian beaver (Castor fiber), the representative of the family Castoridae, which includes two living species of the genus Castor. Beavers are the keystone species, also deservedly known as the ecosystem engineer for the ability to create new habitats and to change the existing landscape. Biology of the Eurasian beaver is well studied, but still, there is a lack of knowledge about the ecology of the species - the relationship with the surrounding environment and biota. These studies were car...

  20. In vitro evaluation of potential bitterness-masking terpenoids from the Canada goldenrod (Solidago canadensis).

    Science.gov (United States)

    Li, Jie; Pan, Li; Fletcher, Joshua N; Lv, Wei; Deng, Ye; Vincent, Michael A; Slack, Jay P; McCluskey, T Scott; Jia, Zhonghua; Cushman, Mark; Kinghorn, A Douglas

    2014-07-25

    In a screening of extracts of selected plants native to Ohio against the human bitterness receptor hTAS2R31, a chloroform-soluble extract of the aerial parts of Solidago canadensis (Canada goldenrod) was determined to have hTAS2R31 antagonistic activity and, thus, was fractionated for isolation of potential bitterness-masking agents. One new labdane diterpenoid, solidagol (1), and six known terpenoids, including two labdane diterpenoids (2 and 3), three clerodane diterpenoids (6β-angeloyloxykolavenic acid, 6β-tigloyloxykolavenic acid, and crotonic acid), and a triterpenoid (longispinogenin), were isolated. Among these compounds, 3β-acetoxycopalic acid (2) was found to be the first member of the labdane diterpene class shown to have inhibitory activity against hTAS2R31 activation (IC50 8 μM). A homology model of hTAS2R31 was constructed, and the molecular docking of 2 to this model indicated that this diterpenoid binds well to the active site of hTAS2R31, whereas this was not the case for the closely structurally related compound 3 (sempervirenic acid). The content of 2 in the chloroform-soluble portion of the methanolic extract of S. canadensis was up to 2.24 g/100 g dry weight, as determined by HPLC.

  1. Isolation, characterization, and mechanistic studies of (-)-alpha-gurjunene synthase from Solidago canadensis.

    Science.gov (United States)

    Schmidt, C O; Bouwmeester, H J; Bülow, N; König, W A

    1999-04-15

    The leaves of the composite Solidago canadensis (goldenrod) were shown to contain (-)-alpha-gurjunene synthase activity. This sesquiterpene is likely to be the precursor for cyclocolorenone, a sesquiterpene ketone present in high amounts in S. canadensis leaves. (-)-alpha-Gurjunene synthase was purified to apparent homogeneity (741-fold) by anion-exchange chromatography (on several matrices), dye ligand chromatography, hydroxylapatite chromatography, and gel filtration. Chromatography on a gel filtration matrix indicated a native molecular mass of 48 kDa, and SDS-PAGE showed the enzyme to be composed of one subunit with a denatured mass of 60 kDa. Its maximum activity was observed at pH 7.8 in the presence of 10 mM Mg2+ and the KM value for the substrate farnesyl diphosphate was 5.5 microM. Over a range of purification steps (-)-alpha-gurjunene and (+)-gamma-gurjunene synthase activities copurified. In addition, the product ratio of the enzyme activity under several different assay conditions was always 91% (-)-alpha-gurjunene and 9% (+)-gamma-gurjunene. This suggests that the formation of these two structurally related products is catalyzed by one enzyme. For further confirmation, we carried out a number of mechanistic studies with (-)-alpha-gurjunene synthase, in which an enzyme preparation was incubated with deuterated substrate analogues. Based on mass spectrometry analysis of the products formed, a cyclization mechanism was postulated which makes it plausible that the synthase catalyzes the formation of both sesquiterpenes. Copyright 1999 Academic Press.

  2. Accumulation and release of 241Am by a macrophyte of the Yenisei River (Elodea canadensis).

    Science.gov (United States)

    Bolsunovsky, A; Zotina, T; Bondareva, L

    2005-01-01

    The source of radioactive contamination of the Yenisei River floodplain, including contamination with transuranic elements, is the Mining-and-Chemical Combine of the Russian Ministry of Atomic Energy, which has for many years been producing weapons-grade plutonium. Transuranic elements have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. This work is an investigation of accumulation and release of 241Am by a submerged macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments. In 2000-2003, laboratory experiments were carried out with biomass of E. canadensis Mich. and filtered river water. The samples were collected from the Yenisei River upstream of the discharge of the Combine's radioactive effluent. The experiments showed that 241Am is accumulated by Elodea biomass: the activity concentration of 241Am can reach 3280+/-240 Bq/g, with the concentration factor for 241Am 16 600+/-2200l/kg. Results of chemical fractionation have proved that in the course of 241Am accumulation by Elodea biomass, 241Am tightly bound to biomass increases from 11% to 27% of the total 241Am in the biomass. Release of 241Am from the decaying Elodea biomass has been evaluated experimentally. By the end of the experiment (lasting up to 127 days), the Elodea plants had lost up to 65% of their initial 241Am activity and the rate of 241Am release into the water environment reached 23 Bq/day.

  3. Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments.

    Science.gov (United States)

    Zotina, Tatiana A; Trofimova, Elena A; Medvedeva, Marina Yu; Dementyev, Dmitry V; Bolsunovsky, Alexander Ya

    2015-10-01

    The toxicity, cytotoxicity, and genotoxicity of bulk sediments from the Yenisei River (Siberia, Russia) were estimated in laboratory bioassays based on several endpoints in the aquatic plant Elodea canadensis. The bottom sediment samples were collected in the Yenisei River upstream and downstream of the sources of chemical and radioactive contamination. The testing revealed different sensitivities of Elodea endpoints to the quality of the bottom sediment: weight of shoots Elodea) was the highest in sediments with chemical pollution, whereas the highest inhibition of toxicity endpoints (shoot and root length) occurred in sediments with the highest level of radioactive pollution. The extreme response of Elodea endpoints to the quality of certain sediment samples may be regarded as related to the possible presence of unknown toxicants. The results show that E. canadensis can be used as an indicator species in laboratory contact testing of bottom sediment. The responses of shoot and root length growth endpoints of Elodea can be recommended as basic sensitivity indicators of bottom sediment toxicity. Analysis of cells carrying abnormal chromosomes in the apical root meristem of Elodea can be performed optionally in the same test to assess the genotoxicity of sediments. © 2015 SETAC.

  4. Accumulation and release of 241Am by a macrophyte of the Yenisei River (Elodea canadensis)

    International Nuclear Information System (INIS)

    Bolsunovsky, A.; Zotina, T.; Bondareva, L.

    2005-01-01

    The source of radioactive contamination of the Yenisei River floodplain, including contamination with transuranic elements, is the Mining-and-Chemical Combine of the Russian Ministry of Atomic Energy, which has for many years been producing weapons-grade plutonium. Transuranic elements have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. This work is an investigation of accumulation and release of 241 Am by a submerged macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments. In 2000-2003, laboratory experiments were carried out with biomass of E. canadensis Mich. and filtered river water. The samples were collected from the Yenisei River upstream of the discharge of the Combine's radioactive effluent. The experiments showed that 241 Am is accumulated by Elodea biomass: the activity concentration of 241 Am can reach 3280 ± 240 Bq/g, with the concentration factor for 241 Am 16 600 ± 2200 l/kg. Results of chemical fractionation have proved that in the course of 241 Am accumulation by Elodea biomass, 241 Am tightly bound to biomass increases from 11% to 27% of the total 241 Am in the biomass. Release of 241 Am from the decaying Elodea biomass has been evaluated experimentally. By the end of the experiment (lasting up to 127 days), the Elodea plants had lost up to 65% of their initial 241 Am activity and the rate of 241 Am release into the water environment reached 23 Bq/day

  5. Projected Future Distribution of Tsuga canadensis across Alternative Climate Scenarios in Maine, U.S

    Directory of Open Access Journals (Sweden)

    Kathleen Dunckel

    2017-08-01

    Full Text Available Climate change is having an impact on forest ecosystems around the world and is expected to alter the suitable habitat of individual tree species. Forest managers require resources about potential impacts of climate change at the regional scale to aid in climate mitigation efforts. By understanding the geographic distribution of changes in suitable habitat, migration corridors can be identified for conservation and active management. With the increased availability of climate projection data, ancillary Geographic Information Systems data, and field observations, modeling efforts at the regional scale are now possible. Here, we modeled and mapped the continuous distribution of Tsuga canadensis throughout the state of Maine at the regional scale(30 m with high precision (89% of pixels had a coefficient of variation ≤ 4.0%. The random forest algorithm was used to create a strong prediction of suitable habitat for the years 2050 and 2100 from both high and low emission climate projections. The results clearly suggest a significant gain in suitable habitat for Tsuga canadensis range with a general northwest expansion.

  6. Nitrogen balance and ruminal assessment in male and female sheeps fed rations containing castor cake under different treatments

    Directory of Open Access Journals (Sweden)

    Rafael Nogueira Furtado

    2014-12-01

    Full Text Available Was evaluated the influence of alternative methods of detoxification of castor cake on nitrogen balance and ruminal evaluation in sheep fed isocaloric and isonitrogenous experimental diets. Twenty sheep (ten males and ten females were used in five treatments (castor cake untreated, treated with limestone, treated with urea, treated with phosphate monodicalcium and autoclaved and four repetitions. For nitrogen balance we used a randomized block design, with the blocks according to the sex and the variables pH and ammonia nitrogen (NH3-N in the experimental design was split plot, with plots in the diets subplots and the collection times (0, 2, 5 and 8 hours postprandial.The treatments did not influence N intake, N urinary, urea and N-urea concentration in plasma. The Urinary N was higher in diet containing castor cake treated with phosphate monodicalcium (FOS when compared with those containing castor cake treated by autoclave (ACL. Already the nitrogen balance (BN was higher in diet ACL when compared to FOS diet. The pH and N-NH3 were within the normal range of sheep, being little affected by diet. Diets with methods of detoxification of castor cake promoted moderate changes in nitrogen balance with emphasis on the castor cake autoclaved and treated with limestone. The males sheep showed higher nitrogen balance than females.

  7. Long-acting poly(DL:lactic acid-castor oil) 3:7-bupivacaine formulation: effect of hydrophobic additives.

    Science.gov (United States)

    Sokolsky-Papkov, Marina; Golovanevski, Ludmila; Domb, Abraham J; Weiniger, Carolyn F

    2011-12-01

    To reduce formulation viscosity of bupivacaine/poly(DL lactic acid co castor oil) 3:7 without increasing bupivacaine release rates. Poly(DL lactic acid) 3:7 was synthesized and bupivacaine formulation prepared by mixing with additives ricinoleic acid or castor oil. In vitro release measurements identified optimum formulation. Anesthetized ICR mice were injected around left sciatic nerve using nerve stimulator with 0.1 mL of formulation. Animals received 10% bupivacaine-polymer formulation with 10% castor oil (p(DLLA:CO)3:7-10% bupi-10% CO) or 15% bupivacaine-polymer with 10% castor oil (p(DLLA:CO)3:7-15% bupi-10% CO). Sensory and motor block were measured. Viscosity of 10% and 15% bupivacaine-p(DLLA:CO)3:7 formulations was reduced using hydrophobic additives; however, castor oil reduced bupivacaine release rates and eliminated burst effect. Less than 10% of the incorporated bupivacaine was released during 6 h, and less than 25% released in 24 h in vitro. In vivo formulation injection resulted in a 24 h motor block and a sensory block lasting at least 72 h. Incorporation of hydrophobic low-viscosity additive reduced viscosity in addition to burst release effects. Bupivacaine-polymer formulation with castor oil additive demonstrated prolonged sensory analgesia in vivo, with reduced duration of motor block.

  8. Efficiency of Castor Oil as a Storage Medium for Avulsed Teeth in Maintaining the Viability of Periodontal Ligament Cells.

    Science.gov (United States)

    Nabavizadeh, Mohammadreza; Abbaszadegan, Abbas; Khodabakhsi, Afrooz; Ahzan, Shamseddin; Mehrabani, Davood

    2018-03-01

    Researchers always seek a new storage medium for avulsed teeth. Castor oil is a vegetable oil with several advantages such as antimicrobial and antioxidant properties, low toxicity, and glutathione preservation capability, low cost, and high availability. The purpose of this study was to evaluate and compare the capacity of castor oil as a new storage medium in preserving the viability of periodontal ligament (PDL) cells compared to Hank's balanced salt solution (HBSS) and milk. Forty freshly extracted human teeth were divided into 3 experimental and 2 control groups. The experimental teeth were stored dry for 30 min and then immersed for 45 min in one of the following media; castor oil, HBSS, and milk. The positive and negative control groups were exposed to 0 min and 2 h of dry time respectively with no immersion in any storage medium. The teeth were then treated with dispase grade II and collagenase and the number of viable PDL cells were counted. Data were analyzed using Kruskal- Wallis test. The percentage of viable cells treated with castor oil, HBSS and milk counted immediately after removal from these media were 46.93, 51.02 and 55.10 % respectively. The statistical analysis revealed that the value for castor oil was significantly lower than HBSS and milk ( p > 0.05). Within the parameters of this study, it appears that castor oil cannot be served as an ideal medium for storage of avulsed tooth. More investigations under in vivo conditions are required to justify the results of this study.

  9. Herbicidas alternativos para controle de biótipos de Conyza bonariensis e C. canadensis resistentes ao glyphosate Alternative herbicides to control glyphosate-resistant biotypes of Conyza bonariensis and C. canadensis

    Directory of Open Access Journals (Sweden)

    M.S. Moreira

    2010-01-01

    Full Text Available Após sucessivos anos, aplicações do herbicida glyphosate em pomares de citros no Estado de São Paulo selecionaram biótipos resistentes de Conyza bonariensis e C. canadensis. Na ocorrência de plantas daninhas resistentes em uma área agrícola, tornam-se necessárias mudanças nas práticas de manejo para obtenção de adequado controle das populações resistentes, bem como para a redução da pressão de seleção sobre outras espécies. Assim, este trabalho foi realizado com o objetivo de identificar herbicidas alternativos para controle de biótipos de Conyza spp. resistentes ao herbicida glyphosate, com aplicações em diferentes estádios fenológicos da planta daninha. Três experimentos foram conduzidos em campo, em pomares de citros em formação, sobre plantas de buva em estádio fenológico de dez folhas e no pré-florescimento. Para plantas no estádio de dez folhas, controle satisfatório foi obtido com aplicações de glyphosate + bromacil + diuron (1.440 + 1.200 + 1.200 g ha-1, glyphosate + atrazina (1.440 + 1.500 g ha-1 e glyphosate + diuron (1.440 + 1.500 g ha-1. Quando em estádio de pré-florescimento de Conyza spp., a aplicação do herbicida amônio-glufosinato, na dose de 400 g ha-1, isolado ou associado a MSMA, bromacil+diuron, metsulfuron, carfentrazone e paraquat, foi a alternativa viável para controle dos biótipos resistentes ao glyphosate.After successive years, glyphosate applications on São Paulo-Brazil citrus orchards selected resistant biotypes of Conyza bonariensis and C. canadensis. The occurrence of herbicide-resistant weed biotypes at some agricultural area makes it necessary to change the management practices to reach effective control of the selected resistant populations, as well as to reduce selection pressure on the other species. Thus, this work aimed to identify the alternative herbicides to control glyphosate-resistant biotypes of Conyza spp., with applications at different weed phenological

  10. Physiological response and differential leaf proteome pattern in the European invasive Asteraceae Solidago canadensis colonizing a former cokery soil.

    Science.gov (United States)

    Immel, Françoise; Renaut, Jenny; Masfaraud, Jean-François

    2012-02-02

    Derelict contaminated sites are often colonized spontaneously by plant species leading to a vegetal cover thought to limit particle dispersal and polluted water infiltration. Those plants must cope with soil pollutants through tolerance mechanisms that are not yet fully understood. Here, we focused our attention on a particular Asteraceae plant, Solidago canadensis, considered as invasive in Europe. S. canadensis spontaneously growing on either polluted (NM soil) or control soils dumped on experimental plots were studied for their physiological status, oxidative stress and 2D-DIGE of leaf extracts. S. canadensis tolerance to soil pollutants was demonstrated since growth rates, allocation to reproduction ratios and Fv/Fm ratios were similar in plants from control and NM soil. At the cell level, the catalase activity level was increased in plants collected on NM soil while lipoperoxidation was unaffected. Also, the leaf proteomic study revealed thirty down-regulated and sixty-six up-regulated proteins. Abundances of proteins related to oxidative stress, carbohydrate metabolism, ion transport were mainly up-regulated while those of proteins involved in cell cycle and transcription/translation were mostly down-regulated. Proteins associated to protein metabolism were either down- or up-regulated. Considered altogether, we highlighted that S. canadensis exhibited a complex proteome response when experiencing a multicontaminated soil. Copyright © 2011 Elsevier B.V. All rights reserved.

  11. Species groups occupying different trophic levels respond differently to the invasion of semi-natural vegetation by Solidago canadensis

    NARCIS (Netherlands)

    Groot, de M.; Kleijn, D.; Jogan, N.

    2007-01-01

    We studied the impact of the invasive plant species Solidago canadensis on the species richness of vascular plants and the abundance, species richness and diversity of butterflies, hoverflies and carabid beetles in herbaceous semi-natural habitats near Ljubljana, Slovenia. The species groups were

  12. Physiological responses of eastern hemlock (Tsuga Canadensis) to biological control and silvicultural release: implications for hemlock restoration

    Science.gov (United States)

    Chelcy F. Miniat; David Zeitlow; Steven T. Brantley; Albert (Bud) Mayfield; Rusty Rhea; Robert Jetton; Paul.  Arnold

    2016-01-01

    The rapid loss of eastern hemlock (Tsuga canadensis) from riparian zones in the southern Appalachian Mountains due to Hemlock Woolly Adelgid (Adelgis tsugae, HWA) infestation has resulted in changes to watershed structure and function. Several restoration strategies have been proposed, including silvicultural treatments that increase incident light in forest...

  13. Testing Methods for Challenging the National Wetland Plant List: Using Tsuga canadensis (L.) Carr. (Eastern Hemlock) as a Case Study

    Science.gov (United States)

    2017-07-01

    frame were constructed based on GIS data (Table 1) and habitat descriptions of areas where T. canadensis was likely to occur, such as wooded swamps...6 2.1 Small study areas (100 km2) ......................................................................................... 6 2.1.1 Constructing a...Western Ecology Division are thanked for their procedural reviews and recommendations for improving the study design. Ms. Lindsey Lefebvre, Ms

  14. Toxoplasmosis in geese and detection of two new atypical Toxoplasma gondii strains from naturally infected Canada geese (Branta canadensis)

    Science.gov (United States)

    The protozoan Toxoplasma gondii infects virtually all warm-blooded animals, including birds, humans, livestock, and marine mammals. The consumption of raw or undercooked meat infected with T. gondii is considered an important source of infection in humans. Canada goose (Branta canadensis), the most ...

  15. Improving germination of red elm (Ulmus rubra), gray alder (Alnus incana), and buffaloberry (Shepherdia canadensis) seeds with gibberellic acid

    Science.gov (United States)

    Brenda Morales; Charles Barden; Cheryl Boyer; Jason Griffin; Lillian Fisher; Joni Thompson

    2012-01-01

    Red elm (Ulmus rubra), gray alder (Alnus incana), and buffaloberry (Shepherdia canadensis) are considered important plants for many Native American tribes in the United States. Native Americans use these 3 species for a variety of traditional and medicinal purposes. For example, red elm is still the preferred firewood for the cultural ceremonies of several tribes....

  16. Production and characterization of MDF using eucalyptus fibers and castor oil-based polyurethane resin

    Directory of Open Access Journals (Sweden)

    Campos Cristiane Inácio de

    2004-01-01

    Full Text Available The growing popularity of wooden panels renders this market segment increasingly competitive. MDF (Medium Density Fiberboard, in particular, is widely employed for a variety of applications, including civil construction, furniture, and packaging. This paper discusses a study of MDF produced from alternative raw materials, i.e., Eucalyptus fibers and castor-oil-based polyurethane resin. Physical and mechanical tests were performed to determine the MDF's modulus of elasticity and modulus of rupture in static bending tests, its swelling, water absorption, moisture and density. The results of the physical and mechanical characterization of this laboratory-produced MDF are discussed and compared with the Euro MDF Board standard. MDF produced with eucalyptus fiber and castor-oil-based polyurethane resin presents results very satisfactory.

  17. Relaxation of potential, flows, and density in the edge plasma of Castor tokamak

    International Nuclear Information System (INIS)

    Hron, M.; Weinzettl, V.; Dufkova, E.; Duran, I.; Stoeckel, J.; Hidalgo, C.

    2004-01-01

    Decay times of plasma flows and plasma profiles have been measured after a sudden biasing switch-off in experiments on the Castor tokamak. A biased electrode has been used to polarize the edge plasma. The edge plasma potential and flows have been characterized by means of Langmuir and Mach probes, the radiation was measured using an array of bolometers. Potential profiles and poloidal flows can be well fitted by an exponential decay time in the range of 10 - 30 μs when the electrode biasing is turned off in the Castor tokamak. The radiation shows a slower time scale (about 1 ms), which is linked to the evolution in the plasma density and particle confinement. (authors)

  18. Comparison of lubricant properties of castor oil and commercial engine oil

    Directory of Open Access Journals (Sweden)

    Binfa Bongfa

    2015-06-01

    Full Text Available The tribological performance of crude Nigeria-based castor oil has been investigated and compared with that of a foreign, 20W-50 high quality crankcase oil, to see its suitability as base oil for lubricating oils in indigenous vehicle and power plants engines. The experiment was conducted using a four ball tester. The results showed that unrefined castor oil has superior friction reduction and load bearing capability in an unformulated form than the commercial oil; can compete favourably with the commercial oil in wear protection when formulated with suitable antiwear agent, hence can be a good alternative base stock for crankcase oils suitable for Nigeria serviced vehicles, and plants engines from tribological, environmental, and non-food competitive points of view.

  19. Analyses to demonstrate the thermal performance of the CASTOR KN12

    International Nuclear Information System (INIS)

    Diersch, R.; Weiss, M.; Tso, C.F.; Powell, D.; Choy, B.I.; Lee, H.Y.

    2004-01-01

    The CASTOR registered KN-12 is a new cask design of GNB for dry and wet transportation of up to 12 PWR spent nuclear fuel assemblies in Korea. It complies with the requirements of 10 CFR 71 [1] and IAEA ST-1 [2] for TYPE B(U)F packages. It received its transport license from the Korean Competent Authority KINS in July 2002 and is now in use in South Korea. Demonstration of the cask's compliance with the regulatory requirements in the area of thermal performance has been carried out by a combination of testing carried out by Korea Atomic Energy Research Institute and analyses carried out by Arup. This paper describes the analyses to demonstrate the thermal performance of the cask and compliance with regulatory requirements under normal and hypothetical accident conditions of transport. Other aspects of the design of the CASTOR registered KN12 are presented in other papers at this conference

  20. Analysis of quality of the biogasoils of palm oil and castor oil

    International Nuclear Information System (INIS)

    Benjumea, Pedro Nel; Agudelo, Jhon Ramiro; Benavides, Alirio Yovany

    2004-01-01

    Biodiesel is a fuel made from raw materials of renewable origin such as vegetable oils. The objective of this work is to make a quality analysis of two types of biodiesel made from raw materials available in Colombia such as palm oil and castor oil. Biodiesel from palm oil complies with the majority of technical requirements specified by ASTM standards D-975 y D-6751. A high cloud point is the main drawback of this kind of biodiesel. This is a consequence of its highly saturated chemical nature. On the other hand, biodiesel from castor oil presents more difficulties in order to be used in diesel engines because of having a low cetane index and a high viscosity

  1. Biodiesel production from castor oil using heterogeneous Ni doped ZnO nanocatalyst.

    Science.gov (United States)

    Baskar, G; Aberna Ebenezer Selvakumari, I; Aiswarya, R

    2018-02-01

    In the present study, castor oil with high free fatty acid was used for biodiesel production using heterogeneous Ni doped ZnO nanocatalyst. Ni doped ZnO nanocomposite calcinated at 800 °C has shown better catalytic activity. Process parameters on heterogeneous catalysis of castor oil into biodiesel were optimized using conventional and Response Surface Methodology (RSM). RSM was found more accurate in estimating the optimum conditions with higher biodiesel yield (95.20%). The optimum conditions for transesterification was found to be oil to methanol molar ratio of 1:8, catalyst loading 11% (w/w), reaction temperature of 55 °C for 60 min of reaction time by response surface method. The reusability studies showed that the nanocatalyst can be reused efficiently for 3 cycles. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Low density polyethylene (LDPE) / poli (3-hydroxy-butyrate) (PHB) blends filled with castor oil cake

    International Nuclear Information System (INIS)

    Rocha, M.C.G.; Oliveira, C.I.R. de; Sanches, M.C.; Coelho, N.N.

    2014-01-01

    Blends of PHB and LDPE were prepared by melt mixing in a Haake internal mixer. Castor oil pressed cake was used as filler for the blends. In order to improve the interfacial adhesion between the filler and the polymers, a mercerization process with 5% NaOH solution was employed. This process was evaluated by several techniques such as: X-Ray diffraction, infrared spectroscopy and scanning electron microscopy (SEM). The mechanical properties were evaluated by traditional tensile stress-strain tests (ASTM D- 638). The obtained results showed that the mercerization process leads to better adhesion properties. The Young Modulus of the blends presented a tendency to increase with the addition of the castor oil cake.(author)

  3. Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.)

    DEFF Research Database (Denmark)

    Nogueira, Fábio C S; Palmisano, Giuseppe; Schwämmle, Veit

    2013-01-01

    In this study, we used a mass spectrometry-based quantification approach employing isotopic (ICPL) and isobaric (iTRAQ) labeling to investigate the pattern of protein deposition during castor oil seed (Ricinus communis L.) development, including that of proteins involved in fatty acid metabolism...... give important insights into certain aspects of the biology of castor oil seed development such as carbon flow, anabolism, and catabolism of fatty acid and the pattern of deposition of SSPs, toxins, and allergens such as ricin and 2S albumins. We also found, for the first time, some genes of SSP......, seed-storage proteins (SSPs), toxins, and allergens. Additionally, we have used off-line hydrophilic interaction chromatography (HILIC) as a step of peptide fractionation preceding the reverse-phase nanoLC coupled to a LTQ Orbitrap. We were able to identify a total of 1875 proteins, and from these 1748...

  4. Biodiesel Production from Castor Oil by Using Calcium Oxide Derived from Mud Clam Shell

    OpenAIRE

    Ismail, S.; Ahmed, A. S.; Anr, Reddy; Hamdan, S.

    2016-01-01

    The catalytic potential of calcium oxide synthesized from mud clam shell as a heterogeneous catalyst for biodiesel production was studied. The mud clam shell calcium oxide was characterized using particle size analyzer, Fourier transform infrared spectroscopy, scanning electron microscopy, and BET gas sorption analyzer. The catalyst performance of mud clam shell calcium oxide was studied in the transesterification of castor oil as biodiesel. Catalyst characterization and transesterification s...

  5. Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds

    Directory of Open Access Journals (Sweden)

    K.V. Fernandes

    2012-11-01

    Full Text Available Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin and allergenic (2S albumin proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death was assessed by cell counting and measurement of lactate dehydrogenase activity. The limit of detection of the Vero cell assay was 10 ng/mL using a concentration of 1.6 x 10(5 cells/well. Solid-state fermentation (SSF and treatment with calcium compounds were used as cake detoxification processes. For SSF, Aspergillus niger was grown using a castor cake as a substrate, and this cake was analyzed after 24, 48, 72, and 96 h of SSF. Ricin was eliminated after 24 h of SSF treatment. The cake was treated with 4 or 8% Ca(OH2 or CaO, and both the toxicity and the allergenic properties were entirely abolished. A by-product free of toxicity and allergens was obtained.

  6. Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, K.V.; Deus-de-Oliveira, N. [Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro, RJ (Brazil); Godoy, M.G. [Laboratório de Biotecnologia Microbiana, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ (Brazil); Guimarães, Z.A.S. [Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro, RJ (Brazil); Nascimento, V.V. [Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro, RJ (Brazil); Melo, E.J.T. de [Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro, RJ (Brazil); Freire, D.M.G. [Laboratório de Biotecnologia Microbiana, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ (Brazil); Dansa-Petretski, M.; Machado, O.L.T. [Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro, RJ (Brazil)

    2012-08-24

    Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death was assessed by cell counting and measurement of lactate dehydrogenase activity. The limit of detection of the Vero cell assay was 10 ng/mL using a concentration of 1.6 × 10{sup 5} cells/well. Solid-state fermentation (SSF) and treatment with calcium compounds were used as cake detoxification processes. For SSF, Aspergillus niger was grown using a castor cake as a substrate, and this cake was analyzed after 24, 48, 72, and 96 h of SSF. Ricin was eliminated after 24 h of SSF treatment. The cake was treated with 4 or 8% Ca(OH){sub 2} or CaO, and both the toxicity and the allergenic properties were entirely abolished. A by-product free of toxicity and allergens was obtained.

  7. Hybrid vigour and gene action for two quantitative traits of castor ...

    African Journals Online (AJOL)

    Five homozygous lines of castor plant, namely RS1-Om, RN1-Om, RTl-2m, RSl- Owm, and RNl- Omb, were crossed to raise F1, F2, BC1, and BC2 generations. The hybrids were tested for hybrid vigour for two metric traits, viz; number of pods per plant and seed yield per plant. Highly significant hybrid vigour was detected ...

  8. Role of biotechnological interventions in the improvement of castor (Ricinus communis L.) and Jatropha curcas L.

    Science.gov (United States)

    Sujatha, M; Reddy, T P; Mahasi, M J

    2008-01-01

    Castor and Jatropha belong to the Euphorbiaceae family. This review highlights the role of biotechnological tools in the genetic improvement of castor and jatropha. Castor is monotypic and breeding programmes have mostly relied on the variability available in the primary gene pool. The major constraints limiting profitable cultivation are: vulnerability to insect pests and diseases, and the press cake is toxic which restrict its use as cattle feed. Conventional breeding techniques have limited scope in improvement of resistance to biotic stresses and in quality improvement owing to low genetic variability for these traits. Genetic diversity was assessed using protein based markers while use of molecular markers is at infancy. In vitro studies in castor have been successful in shoot proliferation from meristematic explants, but not callus-mediated regeneration. Genetic transformation experiments have been initiated for development of insect resistant and ricin-free transgenics with very low transformation frequency. In tropical and subtropical countries jatropha is viewed as a potential biofuel crop. The limitations in available germplasm include; lack of knowledge of the genetic base, poor yields, low genetic diversity and vulnerability to a wide array of insects and diseases. Great scope exists for genetic improvement through conventional methods, induced mutations, interspecific hybridization and genetic transformation. Reliable and highly efficient tissue culture protocols for direct and callus-mediated shoot regeneration and somatic embryogenesis are established for jatropha which indicates potential for widening the genetic base through biotechnological tools. Assessment of genetic diversity using molecular markers disclosed low interaccessional variability in local Jatropha curcas germplasm. The current status and future prospects of in vitro regeneration, genetic transformation and the role of molecular tools in the genetic enhancement of the two

  9. The Energy Deposition Pattern as the Unconventional Strangelet Signature and its Relevance to the Castor Calorimeter

    International Nuclear Information System (INIS)

    Angelis, A.L.S.; Bartke, J.; Gladysz-Dziadus, E.; Wlodarczyk, Z.

    1998-07-01

    It has been shown, by GEANT simulations, that the energy deposition pattern in deep calorimeters could be the spectacular and unconventional signature of different kinds of stable and unstable strangelets. The CASTOR calorimeter is shown to be the appropriate tool for detection of strongly penetrating objects, such as strangelets possibly produced in the baryon-rich region in central Pb-Pb collisions at LHC energies. (author)

  10. Antimicrobial action of sodium hypochlorite and castor oil solutions for denture cleaning – in vitro evaluation

    Directory of Open Access Journals (Sweden)

    Marcela Moreira SALLES

    2015-01-01

    Full Text Available The objective of this in vitro study was to evaluate the antimicrobial action of sodium hypochlorite (0.25% and 0.50% and 10% castor oil solutions against specific microorganisms, by counting Colony Forming Units (CFU of clinically important bacteria and Candida species. Acrylic resin specimens (n = 320; Lucitone 550 were obtained from square metal matrices (10 x 10 x 2 mm, sterilized by microwave (650W, for 6 minutes and contaminated by Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Bacillus subtilis, Escherichia coli, Streptococcus mutans, Enterococcus faecalisand Candida glabrata. The specimens were immersed for 20 minutes in one of the following hygiene solutions (n = 10/each: A – 0.25% Sodium hypochlorite; B – 0.5% Sodium hypochlorite; C – 10% Castor oil solution; and D (Control – saline. Adhered cells were suspended and inoculated into a selective solid medium (37ºC for 24 h. The Student’s t-test (α = 0.05 was performed to compare log10(CFU+1/mL between Groups C and D. The results showed that sodium hypochlorite (0.25% and 0.5% completely eliminated all detectable microorganisms. The castor oil solution eliminatedB. subtilisand reduced counts for other strains. Differences between C and D were significant (p E. faecalis. Both sodium hypochlorite solutions (0.25% and 0.5% were effective in eliminating all microorganisms evaluated, and may be useful as cleaning solutions for complete dentures. The castor oil solution provided moderate efficacy and performed differently on the tested species, with the strongest effect on B. subtilis and with non-significant action on E. faecalis.

  11. Experimental and numerical study of palm oil and castor oil biodiesel droplet evaporation

    OpenAIRE

    Botero, M.L; Molina, A.

    2017-01-01

    ABSTRACT: The vaporization characteristics of Palm and Castor oil biodiesel (Ricinus comunis) droplets were studied. An experimental set-up for measuring the evaporation rate of fuel droplets at atmospheric pressure and variable temperatures was developed. The droplets were suspended on a quartz fiber with initial droplet diameters ranging from 0.9 mm to 1.3 mm. The D2 law model for droplet evaporation was used to predict the evaporation rate of the fuels. Biodiesel physical properties were e...

  12. Magnetic measurements using array of integrated Hall sensors on the CASTOR tokamak

    Czech Academy of Sciences Publication Activity Database

    Ďuran, Ivan; Hronová-Bilyková, Olena; Stöckel, Jan; Sentkerestiová, J.; Havlíček, Josef

    2008-01-01

    Roč. 79, č. 10 (2008), 10F123-10F123 ISSN 0034-6748. [Topical Conference on High-Temperature Plasma Diagnostics/17th./. Albuquerque, 11.05.2008-15.05.2008] R&D Projects: GA MPO 2A-1TP1/101 Institutional research plan: CEZ:AV0Z20430508 Keywords : Galvanomagnetic Sensor * Fusion Reactor * Magnetic Diagnostics * CASTOR tokamak Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders Impact factor: 1.738, year: 2008

  13. Influence of phosphorous fertilization on copper phytoextraction and antioxidant defenses in castor bean (Ricinus communis L.).

    Science.gov (United States)

    Huang, Guoyong; Rizwan, Muhammad Shahid; Ren, Chao; Guo, Guangguang; Fu, Qingling; Zhu, Jun; Hu, Hongqing

    2018-01-01

    Application of fertilizers to supply appropriate nutrients has become an essential agricultural strategy for enhancing the efficiency of phytoremediation in heavy metal contaminated soils. The present study was conducted to investigate the beneficial effects of three types of phosphate fertilizers (i.e., oxalic acid-activated phosphate rock (APR), Ca(H 2 PO 4 ) 2 , and NaH 2 PO 4 ) in the range of 0-600 mg P kg -1 soil, on castor bean growth, antioxidants [antioxidative enzymes and glutathione (GSH)], and Cu uptake. Results showed that with the addition of phosphorus fertilizers, the dry weight of castor bean and the Cu concentration in roots increased significantly, resulting in increased Cu extraction. The phosphorus concentration in both shoots and roots was increased as compared with the control, and the Ca(H 2 PO 4 ) 2 treatment had the greatest effect. Application of APR, NaH 2 PO 4 , and Ca(H 2 PO 4 ) 2 reduced the malondialdehyde (MDA) content, and the activity of the two antioxidant enzymes superoxide dismustase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6) in the leaves of castor bean. GSH concentration in leaves increased with the increasing levels of phosphorus applied to soil as well as the accumulation of phosphorus in shoots, compared to the control. These results demonstrated that the addition of phosphorus fertilizers can enhance the resistance of castor bean to Cu and increase the Cu extraction efficiency of the plant from contaminated soils.

  14. Characterization of adhesive of polyurethane from castor oil by FTIR, TGA and XRD

    International Nuclear Information System (INIS)

    Azevedo, Elaine C.; Santana, Simone S. de M.; Nascimento, Eduardo M. do; Claro Neto, Salvador; Lepienski, Carlos M.

    2009-01-01

    Castor oil polyurethanes are an alternative to adhesives that emanate volatile compounds. This adhesive come from a renewable source and has very low toxicity. In this work the microstructural and thermal characterization is presented. This material is partially crystalline. The mass loss start at 240 deg C and the glass transition temperature is 60 deg C. Then the adhesive is adequate to be employed at temperatures lower than 60 deg C. (author)

  15. Glutathione Preservation during Storage of Rat Lenses in Optisol-GS and Castor Oil

    DEFF Research Database (Denmark)

    Holm, Thomas; Brøgger-Jensen, Martin Rocho; Johnson, Leif

    2013-01-01

    Glutathione concentration in the lens decreases in aging and cataractous lenses, providing a marker for tissue condition. Experimental procedures requiring unfrozen lenses from donor banks rely on transportation in storage medium, affecting lens homeostasis and alterations in glutathione levels. ....... The aim of the study was to examine the effects of Optisol-GS and castor oil on lens condition, determined from their ability to maintain glutathione concentrations....

  16. Cadmium sulfide quantum dots stabilized by castor oil and ricinoleic acid

    Science.gov (United States)

    Kyobe, Joseph William; Mubofu, Egid Beatus; Makame, Yahya M. M.; Mlowe, Sixberth; Revaprasadu, Neerish

    2016-02-01

    Castor oil and ricinoleic acid (an isolate of castor oil) are environmentally friendly bio-based organic surfactants that have been used as capping agents to prepare nearly spherical cadmium sulfide quantum dots (QDs) at 230, 250 and 280 °C. The prepared quantum dots were characterized by Ultra violet-visible (UV-vis), Photoluminescence (PL), Transmission Electron Microscopy (TEM), High Resolution Transmission Electron Microscopy (HRTEM) and X-ray diffraction (XRD) giving an overall CdS QDs average size of 5.14±0.39 nm. The broad XRD pattern and crystal lattice fringes in the HRTEM images showed a hexagonal phase composition of the CdS QDs. The calculated/estimated average size of the prepared castor oil capped CdS QDs for various techniques were 4.64 nm (TEM), 4.65 nm (EMA), 5.35 nm (UV-vis) and 6.46 nm (XRD). For ricinoleic acid capped CdS QDs, the average sizes were 5.56 nm (TEM), 4.78 nm (EMA), 5.52 nm (UV-vis) and 8.21 nm (XRD). Optical properties of CdS QDs showed a change of band gap energy from its bulk band gap of 2.42-2.82 eV due to quantum size confinement effect for temperature range of 230-280 °C. Similarly, a blue shift was observed in the photoluminescence spectra. Scanning electron microscope (SEM) observations show that the as-synthesized CdS QDs structures are spherical in shape. Fourier transform infra-red (FTIR) studies confirms the formation of castor oil and ricinoleic acid capped CdS QDs.

  17. Nanoparticle Structures with (Un-)Hydrogenated Castor Oil as Hydrophobic Paper Coating.

    Science.gov (United States)

    Samyn, Pieter; Vonck, Leo; Stanssens, Dirk; Abbeele, Henk Van den

    2018-05-01

    The encapsulation of vegetable oils within an aqueous dispersion of polymer nanoparticles provides an alternative route to create functional paper coatings from renewable resources, by combining the presentation of hydrophobic moieties together with variations in roughness at the paper surface. The effects of two selected vegetable oil types, i.e., castor oil and hydrogenated castor oil (wax), are compared in terms of nanoparticle synthesis, coating hydrophobicity and surface gloss. The nanoparticles were synthesized by adding 50 wt.-% oil during imidization of poly(styrene-co-maleic anhydride) with ammonium hydroxide. From evaluation of the thermal properties, the nanoparticles have a high glass transition temperature that is suppressed in presence of oil. The nanoparticles with hydrogenated castor oil have higher imide content and better thermal stability compared to castor oil, in parallel with lower chemical reactivity of the hydrogenated oil and less interference with the imidization reaction. After deposition as a coating on paper, the physical coating properties are discussed in parallel with the coating chemistry and morphology or roughness at different scale lengths. The nanoparticle coatings with hydrogenated oil provides a multi-scale roughness with an open, porous nanoparticles structures and presentation of some amount free oil augmenting hydrophobicity towards a water contact angle of 128° (static contact angle) or 138° (advancing contact angle). The differences in surface coverage of coated papers in terms of imide and oil contents are confirmed by chemical Raman mapping. The differences in surface roughness are confirmed by non-contact profilometry, laser interferometry and atomic force microscopy.

  18. Characterization and Digestibility of Detoxified Castor Oil Meal for Japanese Quails

    OpenAIRE

    Santos,PA dos; Ludke,MCMM; Ludke,JV; Rabello,CBV; Santos,MJB dos; Torres,TR

    2015-01-01

    ABSTRACT These experiments were performed to determine the chemical composition, coefficients of nutrient and energy metabolizability, amino acid composition, and cytotoxicity of different castor oil meals subjected to different detoxification processes and added to the diet of Japanese quails. In the trial, 180 46-d-old female Japanese quails were distributed according to a completely randomized design into five treatments and with replicates of six bird each. The treatments consisted of fol...

  19. Formation of convective cells in the scrape-off layer of the Castor tokamak

    International Nuclear Information System (INIS)

    Stoeckel, J.; Hron, M.; Adamek, J.; Brotankova, J.; Dejarnac, R.; Duran, I.; Panek, R.; Stejskal, P.; Zacek, F.; Devynck, P.; Gunn, J.; Martines, E.; Bonhomme, G.; Van Oost, G.; Hansen, T.; Gorler, T.; Svoboda, V.

    2004-01-01

    We describe experiments with a biased electrode inserted into the scrape-off layer (SOL) of the CASTOR tokamak. The resulting radial and poloidal electric field and plasma density modification are measured by means of Langmuir probe arrays with high temporal and spatial resolutions. Poloidally and radially localized stationary structures of the electric field (convective cells) are identified and a related significant modification of the particle transport in the SOL is observed. (authors)

  20. Down regulation of APETALA3 homolog resulted in defect of floral structure critical to explosive pollen release in Cornus canadensis

    Institute of Scientific and Technical Information of China (English)

    Xiang Liu; Lu Li; Qiu-Yun (Jenny) Xiang

    2017-01-01

    In mature buds of the dwarf dogwood lineage (DW) of Cornus,petals and filaments form an "x"-like box containing mechanical energy from the filaments to allow explosive pollen dispersal.As a start to understand the molecular mechanisms responsible for the origin of this unique structure in Cornus,we cloned and characterized the sequences of APETALA3 (AP3) homologs from Cornus canadensis of the DW lineage and five other Cornus species,given the function of AP3 on petal and stamen development in Arabidopsis,and tested the function of CorcanAP3 using a stable Agrobacterium-mediated transformation system.The cloned CorAP3s (AP3-like genes in Cornus) were confirmed to belong to the euAP3 lineage.qRT-PCR analysis indicated strong increase of CorcanAP3 expression in floral buds of wildtype C.canadensis.A hairpin construct of CorcanAP3 was successfully introduced into wild type plants of C.canadensis,resulting in significant reduction of CorcanAP3 expression and abnormal floral development.The abnormal floral buds lost the "x" form and opened immaturely due to delay or retard of petal and stamen elongation and the push of style elongation.The results suggested CorcanAP3 may function to regulate the coordinated rate of development of petals and stamens in C.canadensis,necessary for the x-structure formation,although the exact molecular mechanism remains unclear.Comparison among six Comus species indicated a greater ratio of stamen to petal and style growth in C.canadensis,suggesting an evolutionary change of CorAP3 expression pattern in the DW lineage,leading to the greater growth of filaments to form the "x"-box.

  1. UV and gamma irradiation effects on surface properties of polyurethane derivative from castor oil

    International Nuclear Information System (INIS)

    Azevedo, Elaine C.; Nascimento, Eduardo M.; Chierice, Gilberto O.; Claro Neto, Salvador

    2013-01-01

    Gamma and ultraviolet radiation effects on hardness, elastic modulus and viscoelastic properties of polyurethane derived from castor oil (PU) were investigated by nanoindentation tests. Modifications on surface morphology, induce by radiation, were observed by atomic force microscopy. The polyurethane derivative from castor oil shows good resistance to gamma radiation, with only small changes in hardness, elastic modulus, viscoelastic properties and contact angle. The hardness of PY increases at the near surface region due to UVA radiation and decreases after UVC radiation. The contact angle for water drop decreases after UVC radiation, by not after gamma radiation, despite a significant increase in roughness. Such results are attributed to different responses from polyurethane to radiation energy. Increase in hardness due to UVA is attributed to a higher crosslinking at shallow depths, while a decrease in mechanical properties may be attributed to chain scission. These results are consistent with the modifications on viscoelastic properties. Shore D hardness did not show the same trend as observed by nanoindentation results. Hardness, viscoelastic properties and contact angle of castor oil polyurethane are more severely influenced by UVC radiation, while gamma radiation does not have a significant effect. (author)

  2. Physicochemical characteristics of seeds from wild and cultivated castor bean plants (Ricinus communis L.

    Directory of Open Access Journals (Sweden)

    Jose Daniel Mosquera

    2018-01-01

    Full Text Available The castor (Ricinus communis L. is an oilseed plant whose main features are its drought resistance, and its adaptation to eroded, polluted, and low fertility soils. Its oil has a great demand in the industrial sector and it has recently attracted considerable interest for its use in the production of biodiesel and jet fuel. In this study, morphological, physical and chemical characterizations were performed to ascertain the quality of wild (VQ-1 and under cultivation (VQ-7 oil castor seeds. The results showed that there are differences in the morphological and physicochemical characteristics regarding oil content (44,95 vs 33,84%, ash (3,20 vs 2,42%, and 100-seed-weight (45,87 vs 54,23g; similar behavior was recorded when characterizing the oil: kinematic viscosity (269,67 vs 266,44mm2 /s, density (0,9389 vs 0,9465g/cm3 , and acidity index (0,9918 vs 0,5440mg KOH/g for VQ-1 and VQ-7, respectively. Growing conditions to which castor plants were subjected may influence both the final quality of seeds and chemical properties of the oil.

  3. Potential of sunflower, castor bean, common buckwheat and vetiver as lead phytoaccumulators

    Directory of Open Access Journals (Sweden)

    Jailson do C. Alves

    2016-03-01

    Full Text Available ABSTRACT Studies concerning the tolerance, absorption and distribution of heavy metals in plants are essential for the success of phytoremediation programs. The present study was carried out in order to evaluate the potential of the sunflower, castor bean, common buckwheat and vetiver as lead phytoaccumulators. The species were grown in nutrient solution containing increasing doses of Pb (0, 50, 100, 200 and 400 mg L-1 during a 30-day exposure period. A completely randomized split-plot design was used, with a 4 x 5 factorial and three replicates. Significant reductions of dry matter of the root, shoot and whole plant were found in the all species under study as a function of the increased Pb doses. Vetiver showed higher tolerance to Pb contamination; sunflower and castor bean had intermediate tolerance and the common buckwheat proved to be the most sensitive species. The concentration and total content of Pb in plant compartments were significantly affected by the increased Pb doses in solution, and higher accumulation of this element was observed, in general, in the roots of the studied species. Common buckwheat proved to be not much promising for Pb-phytoremediation programs; sunflower showed potential for Pb phytoextraction and castor bean and vetiver were the most appropriate for Pb phytostabilization.

  4. Alternative castor oil-based polyurethane adhesive used in the production of plywood

    Directory of Open Access Journals (Sweden)

    Fabricio Moura Dias

    2004-09-01

    Full Text Available Plywood is normally produced with urea-formaldehyde and/or phenol-formaldehyde adhesives. However, the former is considerably toxic and environmentally damaging, while the latter is expensive, thus motivating the search for alternative raw materials in plywood production. The castor oil-based polyurethane adhesive developed at the São Carlos Institute of Chemistry, University of São Paulo, is an environmentally friendly vegetal oil-based polymer that is harmless to humans. The wood species Eucalyptus grandis offers favorable properties for plywood the manufacture. The study reported on here involved the use of castor oil-based polyurethane adhesive to produce plywood with Eucalyptus grandis layers. The plywood's performance was evaluated based on the results of physical and mechanical tests recommended by the Brazilian code, ABNT. Tests results showed higher values than those reported in the literature and recommended by the ABNT, indicating that the castor oil-based polyurethane adhesive is a promising glue for the manufacture of plywood.

  5. UV and gamma irradiation effects on surface properties of polyurethane derivative from castor oil

    Energy Technology Data Exchange (ETDEWEB)

    Azevedo, Elaine C.; Nascimento, Eduardo M., E-mail: helunica@yahoo.com.br [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Chierice, Gilberto O.; Claro Neto, Salvador [Universidade de Sao Paulo (IQSC/USP), Sao Carlos, SP (Brazil). Instituto de Quimica; Lepienski, Carlos M. [Universidade Federal do Parana (UFPR), Curitiba (Brazil)

    2013-07-01

    Gamma and ultraviolet radiation effects on hardness, elastic modulus and viscoelastic properties of polyurethane derived from castor oil (PU) were investigated by nanoindentation tests. Modifications on surface morphology, induce by radiation, were observed by atomic force microscopy. The polyurethane derivative from castor oil shows good resistance to gamma radiation, with only small changes in hardness, elastic modulus, viscoelastic properties and contact angle. The hardness of PY increases at the near surface region due to UVA radiation and decreases after UVC radiation. The contact angle for water drop decreases after UVC radiation, by not after gamma radiation, despite a significant increase in roughness. Such results are attributed to different responses from polyurethane to radiation energy. Increase in hardness due to UVA is attributed to a higher crosslinking at shallow depths, while a decrease in mechanical properties may be attributed to chain scission. These results are consistent with the modifications on viscoelastic properties. Shore D hardness did not show the same trend as observed by nanoindentation results. Hardness, viscoelastic properties and contact angle of castor oil polyurethane are more severely influenced by UVC radiation, while gamma radiation does not have a significant effect. (author)

  6. Mechanical evaluation of bone gap filled with rigid formulations castor oil polyurethane and chitosan in horses

    Directory of Open Access Journals (Sweden)

    Rodrigo Crispim Moreira

    Full Text Available ABSTRACT: Often fractures of long bones in horses are comminuted and form bone gaps, which represent a major challenge for the fixation of these fractures by loss of contact between the fragments. Bone grafts help in treating this kind of fracture and synthetic materials have been gaining ground because of the limitations of autologous and heterologous grafts. In this study were performed compressive non destructive test in 10 bones with complete cross-bone gap in mid-diaphyseal of the third metacarpal bone of horses. Using a mechanism of "crossing" the 10 bones were used in the three groups (control, castor oil poliuretane and chitosan according to the filling material. After the test with maximum load of 1000N bone had a gap filled by another material and the test was repeated. Deformations caused on the whole bone, plate and bone tissue near and distant of gap were evaluated, using strain gauges adhered to the surface at these locations. There was a reduction in bone deformation from 14% (control to 3,5% and 4,8% by filling the gap with Chitosan and castor oil respectively, and a reduction of strain on the plate of 96% and 85% by filling gap with chitosan and castor respectively. An increase in intensity and direction of deformations occurred in bone near to gap after its filling; however, there was no difference in bone deformations occurring far the gap.

  7. Organic cultivation of onion under castor cake fertilization and irrigation depths

    Directory of Open Access Journals (Sweden)

    Gabriel Alves Botelho de Mello

    2017-11-01

    Full Text Available Two experiments were carried out to evaluate the effects that different doses of castor bean (0, 200, and 300 g m-2 and irrigation levels (204, 224, 278, and 321 mm in 2014, and 278, 302, 397, and 444 mm in 2015 have on both the productivity and quality of onion bulbs. In the experiments, the experimental design was randomized blocks (4 x 3 factorial scheme, with five replicates. Irrigation management was performed using a Simplified Irrigation Device (SID in response to soil water tension in the treatment of highest irrigation depth. The following variables were evaluated: plant dry biomass (PDB, bulb dry biomass (BDB, total yield (TY, mean bulb fresh weight (MBFW, mean bulb dry weight (MBDW, mean bulb diameter (MBD and water use efficiency (WUE. The highest irrigation depths positively influenced the mean production of onion bulbs, regardless of the applied dose of castor cake. The doses of castor cake positively influenced the production of onion bulbs when higher irrigation depths were applied.

  8. Experiment and analysis of CASTOR type model cask for verification of radiation shielding

    Energy Technology Data Exchange (ETDEWEB)

    Hattori, Seiichi; Ueki, Kohtaro.

    1988-08-01

    The radiation shielding system of CASTOR type cask is composed of the graphite cast iron and the polyethylene lod. The former fomes the cylndrical body of the cask to shield gamma rays and the latter is embeded in the body to shield neutrons. Characteristic of radiation shielding of CASTOR type cask is that zigzag arrangement of the polyethylene lod is adopted to unify the penetrating dose rate. It is necessary to use the three-dimensional analysis code to analyse the shielding performance of the cask with the complicated shielding system precisely. However, it takes too much time as well as too much cost. Therefore, the two-dimensional analysis is usually applied, in which the three-dimensional model is equivalently transformed into the two-dimensional calculation. The reseach study was conducted to verify the application of the two-dimensional analysis, in which the experiment and the analysis using CASTOR type model cask was perfomed. The model cask was manufactured by GNS campany in West Germany and the shielding ability test facilities in CRIEPI were used. It was judged from the study that the two-dimensional analysis is useful means for the practical use.

  9. Yield of castor bean fertilized with sewage sludge and potassium and magnesium sulphate

    Directory of Open Access Journals (Sweden)

    Thâmara F. M. Cavalcanti

    2015-08-01

    Full Text Available ABSTRACTThe aim of this study was to evaluate the yield and nutrition of castor bean in response to fertilization with sewage sludge and potassium (K and magnesium (Mg sulphate. The experiment was carried out from January to July 2011. The treatments, in a randomized block design with three replicates, in a Nitosol, corresponded to a factorial scheme (2 x 4 +1: two doses of K and Mg sulphate combined with four doses of sewage sludge (0, 2.60, 5.20 and 10.40 t ha-1, dry basis, applied based on its nitrogen (N content and the N requirement for the crop and an additional treatment with NPK. The castor bean grain yield fertilized with sewage sludge did not differ from conventional fertilization, with the maximum value achieved at a dose of 7.5 t ha-1 of sewage sludge. The fertilization with sewage sludge increased zinc and copper levels in the soil to values close to or higher than those in conventional fertilization, without any influence on the concentrations in the leaf. Fertilization with K and Mg sulphate increased the levels of these cations in the soil without affecting the concentrations in the leaves. The fertilization with sewage sludge increased the contents of organic matter, sulfur, zinc, iron, copper and boron in the soil, and manganese and boron in castor bean leaves.

  10. Performance of compression ignition engine with indigenous castor oil bio diesel in Pakistan

    International Nuclear Information System (INIS)

    Chakrabarti, M.H.

    2009-01-01

    Castor oil available indigenously in Pakistan was converted successfully to bio diesel and blended to 10% quantity (by volume) with high speed mineral diesel (HSD) fuel. This fuel was tested in a compression-ignition engine in order to assess its environmental emissions as well as engine performance parameters. The blended fuel was found to give lower environmental emissions in most accounts except for higher CO/sub 2/ and higher NOx. In addition, three engine performance parameters were assessed; which were engine brake power, engine torque and exhaust temperature. In the first two cases, blended bio diesel fuel gave lower figures than pure mineral diesel due to lower calorific value. However, its higher flash point resulted in higher engine exhaust temperatures than pure mineral diesel. Overall, in terms of engine performance, castor oil bio diesel (from non edible oil of castor bean -growing on marginal lands of Pakistan) fared better in comparison to canola oil bio diesel (from expensive edible oil) and can be recommended for further tests at higher blend ratios. (author)

  11. Characteristics and Composition of a High Oil Yielding Castor Variety from Pakistan.

    Science.gov (United States)

    Panhwar, Tarique; Mahesar, Sarfaraz Ahmed; Mahesar, Abdul Waheed; Kandhro, Aftab Ahmed; Talpur, Farah Naz; Laghari, Zahid Hussain; Chang, Abdul Sattar; Hussain Sherazi, Syed Tufail

    2016-01-01

    Keeping in view the versatile applications of castor oil in cosmetic, pharmaceutical and recently as renewable source, the present work is a step towards the commercialization of castor on large scale in Pakistan. The current study introduces a castor variety with high oil content. Initially seeds were physically examined for some physical parameters. Seed moisture, ash content and linear dimensions such as length, width and thickness were found to be 4.53%, 6.44%, 12.24 mm, 8.31 mm and 5.67 mm, respectively. For oil extraction, Soxhlet method was used which resulted in the high oil content 54.0%. For quality assessment of oil, physicochemical parameters were checked according to official standard AOCS methods and compared with ASTM specifications. The determined parameters were as follows; specific gravity 0.953 g/cm(-3), refractive index 1.431, viscosity 672.0 mPas.s, moisture content 0.32%, FFA 0.14%, IV 83.61 gI2/100 g, PV 2.25 meq/Kg and SV 186.0 mgKOH/g. Furthermore, fatty acid analysis of oil showed that, most abundant fatty acid was ricinoleic acid 94.59%, followed by palmitic 0.31%, linoleic 1.84%, oleic (n-9) 2.05%, oleic (n-10) 0.22%, stearic 0.45% and eicosenoic acid 0.53%. The detected fatty acids were compared with registered variety and varieties of other regions.

  12. Synthesis and characterization of dialkanolamides from castor oil (Ricinus communis) as nonionic surfactant

    Science.gov (United States)

    Anwar, M.; Wahyuningsih, T. D.

    2017-12-01

    Nonionic surfactant of dialkanolamide derivates was synthesized and characterized from castor oil (Ricinus comunnis). Ricinoleic acid was isolated from castor oil by hydrolysis in alkaline (KOH) condition at 65 °C. Oxidation of ricinoleic acid by dilute potassium permanganate (KMnO4) in alkaline condition at 75-90 °C gave dicarboxylic acid which was then reacted with ethanolamine at 140-160 °C for 6 hours. The product was recrystallized with isopropanol, and the structure elucidation was performed by FTIR, 1HNMR spectrometer, and GC-MS with silylation method. Characterization of surfactants was carried out by surface tension measurement (capillary rise method), Critical Micelle Concentration (CMC) based on turbidity method and calculation of Hydrophilic-Lipophilic Balance (HLB) value with Griffin method and Bancroft rule. The result showed that ricinoleic acid in castor oil is 86.19 % and it is oxidation give an azelaic acid and octanedioic acid in 53.25 %. Amidation of a dicarboxylic acid and ethanolamine at 140-160 °C for 6 hours yielded of N1,N9-bis(2-hydroxyethyl)nona diamide in 49.35 %. Surfactant characterization indicates that dialkanolamide derivates can be used as a surfactant due to its ability to reduce the surface tension of ethanol with CMC at 1.2 g/L, HLB value is 5.58 and can be used as emulsifier water in oil (W/O).

  13. Relocation of the Air National Guard 176th Wing to Elmendorf AFB, Alaska

    Science.gov (United States)

    2007-09-01

    Park. Between 20 and 70 moose are estimated by Alaska Fish and Game to live on Elmendorf AFB, depending on the time of year, as portions of the herd ...also supports populations of small mammals including beaver (Castor canadensis), muskrat (Ondatra zibethicus), porcupine (Erethizon dorsatum), red

  14. The population dynamics of goldenseal by habitat type on the Hoosier National Forest

    Science.gov (United States)

    S. P. Meyer; G. R. Parker

    2003-01-01

    Goldenseal (Hydrastis canadensis L.) is an herbaceous species found throughout the central hardwood forest ecosystem that is harvested from the wild for the medicinal herb trade. A total of 147 goldenseal populations were classified according to the Ecological Classification Guide developed for the Hoosier National Forest, and change in population...

  15. Castor and Pollux - shielded cells for studying fuel treatment processes; Castor et Pollux chaines blindees d'etudes de procedes de traitement de combustibles

    Energy Technology Data Exchange (ETDEWEB)

    Faudot, G; Bathellier, A [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

    1969-07-01

    CASTOR and POLLUX, two alpha, beta, gamma cells are described in the present paper. They are located in the CEN at Fontenay-aux-Roses (France). They are designed for improvement studies of the various aqueous separation processes used in irradiated fuels reprocessing plants. Located in the same air-tight steel encasement, they arc inter-connected by a pneumatic transfer. These two cells have a similar in-line conception and they include: a gamma shielding in lead of 10 cm of thickness; an inner air-tight box, made with stainless steel and plexiglas, is maintained in lowering in comparison to room pressure. Eleven Hobson model seven master-slave manipulators allow inner manipulations. Then the inner equipment is described briefly. (author) [French] Le present document decrit les cellules alpha, beta, gamma CASTOR et POLLUX edifiees au Centre d'Etudes Nucleaires de Fontenay-aux-Roses. Elles sont destinees aux etudes visant a l'amelioration des procedes de separation par voie aqueuse utilises dans les usines de retraitement des combustibles irradies. Ces deux chaines, implantees dans le meme caisson et reliees par convoyeur pneumatique, sont de conception identique et comprennent: une protection biologique constituee par 10 cm d'epaisseur de plomb; une enceinte interieure etanche alpha, en acier inoxidable et plexiglas, maintenue en depression. Des telemanipulateurs Hobson, modele 7, permettent les manipulations interieures. On decrit ensuite brievement les installations annexes. (auteur)

  16. Development of an HPLC post-column antioxidant assay for Solidago canadensis radical scavengers.

    Science.gov (United States)

    Marksa, Mindaugas; Radušienė, Jolita; Jakštas, Valdas; Ivanauskas, Liudas; Marksienė, Rūta

    2016-01-01

    The aim of this work was to modify and validate the post-column high-performance liquid chromatography (HPLC)-ABTS and DPPH methods for evaluating the antioxidant activity of the methanolic extracts of Solidago canadensis (Canadian goldenrod) leaves and flowers. Separation of the analytes was performed via the HPLC-PDA method on a YMC analytical column using a gradient elution program. Three compounds with antioxidant properties - chlorogenic acid, rutin and isoquercitrin - and two unidentified antioxidants were established. The research showed that the coil temperature regimes and loop length combinations influence the optimised post-column assay method for detecting the antioxidant activity of goldenrod radical scavengers. Investigations established that the temperature in the reaction coil was a substantial factor contributing to the signal strength of the analytes after reacting with the DPPH and ABTS radicals.

  17. New lupane triterpenoids from Solidago canadensis that inhibit the lyase activity of DNA polymerase beta.

    Science.gov (United States)

    Chaturvedula, V S Prakash; Zhou, Bing-Nan; Gao, Zhijie; Thomas, Shannon J; Hecht, Sidney M; Kingston, David G I

    2004-12-01

    Bioassay-directed fractionation of a methyl ethyl ketone extract of Solidago canadensis L. (Asteraceae), using an assay to detect the lyase activity of DNA polymerase beta, resulted in the isolation of the four new lupane triterpenoids 1-4 and the seven known compounds lupeol, lupeyl acetate, ursolic acid, cycloartenol, cycloartenyl palmitate, alpha-amyrin acetate, and stigmasterol. The structures of the new compounds were established as 3beta-(3R-acetoxyhexadecanoyloxy)-lup-20(29)-ene (1), 3beta-(3-ketohexadecanoyloxy)-lup-20(29)-ene (2), 3beta-(3R-acetoxyhexadecanoyloxy)-29-nor-lupan-20-one (3), and 3beta-(3-hetohexadecanoyloxy)-29-nor-lupan-20-one (4), respectively, on the basis of extensive 1D and 2D NMR spectroscopic interpretation and chemical modification studies. All 11 compounds were inhibitory to the lyase activity of DNA polymerase beta.

  18. Echinococcus canadensis, E. borealis, and E. intermedius. What's in a name?

    Science.gov (United States)

    Lymbery, Alan J; Jenkins, Emily J; Schurer, Janna M; Thompson, R C Andrew

    2015-01-01

    The phylogenetic relationships of the G6, G7, G8, and G10 genotypes of Echinococcus granulosus are well defined, but their taxonomic status is currently unresolved. We apply an evolutionary species concept to infer that the G6 and G7 genotypes represent a single species that is different to both the G8 and G10 genotypes, and that the G8 and G10 genotypes are also on different evolutionary trajectories and, therefore, should be regarded as separate species. The names Echinococcus intermedius, Echinococcus canadensis, and Echinococcus borealis have been previously proposed for these three taxa (G6/7, G10 and G8, respectively) and we argue that it may be appropriate to resurrect these names. The correct delimitation and formal recognition of species of Echinococcus may have important veterinary and public health consequences. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Antifungal Activity and Composition of Essential Oils of Conyza canadensis Herbs and Roots

    Directory of Open Access Journals (Sweden)

    Katalin Veres

    2012-01-01

    Full Text Available Essential oils from herbs and roots of Conyza canadensis (horseweed, collected in Hungary, were obtained by hydrodistillation. The chemical compositions of the oils were analysed by combination of GC and GC/MS. The major constituent of the oil obtained from the aerial parts of horseweed was limonene (78%, while the main component of root oil was 2Z,8Z-matricaria ester. The antimicrobial activities of the oils were tested on Gram-positive (Enterococcus faecalis, Staphylococcus aureus, and Streptococcus pyogenes, Gram-negative (Escherichia coli, Pseudomonas aeruginosa bacteria, reference fungal strains, and fungal strains isolated from patients (Candida, Cryptococcus, Trichophyton, Rhodotorula, and Aspergillus by agar disc diffusion and broth dilution methods. None of the oils showed any activity against the tested bacterial strains, but exhibited moderate-to-strong activity against all fungi with the only exception of A. fumigatus. The highest zone of inhibition was observed in case of Cryptococcus neoformans and Trichophyton interdigitalis

  20. [Isolation and structure elucidation of further new saponins from Solidago canadensis].

    Science.gov (United States)

    Reznicek, G; Jurenitsch, J; Freiler, M; Korhammer, S; Haslinger, E; Hiller, K; Kubelka, W

    1992-02-01

    Four new main saponins (canadensis-saponins 5-8) (compounds 5-8) were isolated from Solidago canadensis L. (Asteraceae). Using GC/MS, FAB-MS, and mainly 2D-NMR techniques their structures were identified as 3-O-[beta-D-glucopyranosyl(1----3)-beta-D- glucopyranosyl]-28-O-[beta-D-galactopyranosyl(1----2)-alpha-L- rhamnopyranosyl-(1----3)-beta-D-xylopyranosyl-(1----4)-[beta-D- xylopyranosyl-(1----3)]-alpha-L-rhamnopyranosyl-(1----2)-[beta-D-apio -D- furanosyl-(1----3)]-beta-D-6-deoxyglucopyranosyl-(1----)]-bayog enin(5),3-O- [beta-D-glucopyranosyl-(1----3)-beta-D-glucopyranosyl]-28-O-[beta-D- galactopyranosyl-(1----2)-alpha-L-rhamnopyranosyl-(1----3)-beta-D- xylopyranosyl-(1----4)-[beta-D-xylopyranosyl-(1----3)]-alpha-L- rhamnopyranosyl-(1----2)-[beta-D-apio-D-furanosyl-(1----3)]- arabinopyranosyl-(1----)]bayogenin(6),3-O-[beta-D-glucopy ran osyl-(1----3)- beta-D-glucopyranosyl]-28-O-[beta-D-galactopyranosyl-(1----2)- alpha-L-rhamnopyranosyl-(1----3)-beta-D-xylopyranosyl-(1----4)-[beta-D- xylopyranosyl-(1----3)]-alpha-L-rhamnopyranosyl-(1----2)-[alpha-L- rhamnopyranosyl-(1----3)]-beta-D-6-deoxyglucopyranosyl-(1----)]-++ +bayogenin (7), and 3-O-[beta-D-glucopyranosyl-(1----3)-beta-D-glucopyranosyl]-28-[O- beta-D-galactopyranosyl-(1----2)-alpha-L-rhamnopyranosyl-(1----3)-beta-D - xylopyranosyl-(1----4)-[beta-D-xylopyranosyl-(1----3)]-alpha-L- rhamnopyranosyl-(1----2)-[alpha-L-rhamnopyranosyl-(1----3)]arabinopyr anosyl - (1----)[-bayogenin (8).

  1. Accumulation and release of {sup 241}Am by a macrophyte of the Yenisei River (Elodea canadensis)

    Energy Technology Data Exchange (ETDEWEB)

    Bolsunovsky, A. [Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036 (Russian Federation)]. E-mail: radecol@ibp.ru; Zotina, T. [Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036 (Russian Federation); Bondareva, L. [Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036 (Russian Federation)

    2005-07-01

    The source of radioactive contamination of the Yenisei River floodplain, including contamination with transuranic elements, is the Mining-and-Chemical Combine of the Russian Ministry of Atomic Energy, which has for many years been producing weapons-grade plutonium. Transuranic elements have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. This work is an investigation of accumulation and release of {sup 241}Am by a submerged macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments. In 2000-2003, laboratory experiments were carried out with biomass of E. canadensis Mich. and filtered river water. The samples were collected from the Yenisei River upstream of the discharge of the Combine's radioactive effluent. The experiments showed that {sup 241}Am is accumulated by Elodea biomass: the activity concentration of {sup 241}Am can reach 3280 {+-} 240 Bq/g, with the concentration factor for {sup 241}Am 16 600 {+-} 2200 l/kg. Results of chemical fractionation have proved that in the course of {sup 241}Am accumulation by Elodea biomass, {sup 241}Am tightly bound to biomass increases from 11% to 27% of the total {sup 241}Am in the biomass. Release of {sup 241}Am from the decaying Elodea biomass has been evaluated experimentally. By the end of the experiment (lasting up to 127 days), the Elodea plants had lost up to 65% of their initial {sup 241}Am activity and the rate of {sup 241}Am release into the water environment reached 23 Bq/day.

  2. Uptake of metals and metalloids by Conyza canadensis L. from a thermoelectric power plant landfill

    Directory of Open Access Journals (Sweden)

    Vukojević Vesna

    2016-01-01

    Full Text Available Fourteen metals and metalloids were determined in Conyza canadensis L. harvested from the fly ash landfill of the thermoelectric power plant “Kolubara” (Serbia. Fly ash samples were collected together with the plant samples and subjected to sequential extraction according to the three-step sequential extraction scheme proposed by the Community Bureau of Reference (BCR; now the Standards, Measurements and Testing Program. The contents of metals and metalloids were determined by inductively coupled plasma optical emission spectrometry (ICP-OES in plant root and the aboveground part and correlated with their contents in the fly ash samples. The bioconcentration factor (BCF and translocation factors (TF were calculated to access uptake of metals from fly ash and their translocation to the aboveground part. Results regarding As revealed that fly ash samples in the proximity of the active cassette had higher amounts of the element. Principal component analysis (PCA showed that As had no impact on the classification of plant parts. BCF for As ranged from 1.44 to 23.8 and varied, depending on the investigated area; TF for As ranged from 0.43 to 2.61, indicating that the plant translocated As from root to shoot. In addition to As, Conyza canadensis L. exhibited efficient uptake of other metals from fly ash. According to the calculated BCF and TF, the plant retained Al, Fe and Cr in the root and translocated Zn, Cd, Cu and As from root to shoot in the course of the detoxifying process. [Projekat Ministarstva nauke Republike Srbije, br. 172030 i br. 172017

  3. Genome-Wide Identification, Evolutionary Analysis, and Stress Responses of the GRAS Gene Family in Castor Beans

    Directory of Open Access Journals (Sweden)

    Wei Xu

    2016-06-01

    Full Text Available Plant-specific GRAS transcription factors play important roles in regulating growth, development, and stress responses. Castor beans (Ricinus communis are important non-edible oilseed plants, cultivated worldwide for its seed oils and its adaptability to growth conditions. In this study, we identified and characterized a total of 48 GRAS genes based on the castor bean genome. Combined with phylogenetic analysis, the castor bean GRAS members were divided into 13 distinct groups. Functional divergence analysis revealed the presence of mostly Type-I functional divergence. The gene structures and conserved motifs, both within and outside the GRAS domain, were characterized. Gene expression analysis, performed in various tissues and under a range of abiotic stress conditions, uncovered the potential functions of GRAS members in regulating plant growth development and stress responses. The results obtained from this study provide valuable information toward understanding the potential molecular mechanisms of GRAS proteins in castor beans. These findings also serve as a resource for identifying the genes that allow castor beans to grow in stressful conditions and to enable further breeding and genetic improvements in agriculture.

  4. Energetic balance of castor oil methyl and ethyl esters; Balanco energetico de esteres metilicos e etilicos de oleo de mamona

    Energy Technology Data Exchange (ETDEWEB)

    Almeida Neto, Jose Adolfo de; Cruz, Rosenira Serpa da; Alves, Jaenes Miranda; Pires, Monica de Moura; Robra, Sabine [Universidade Estadual de Santa Cruz, Ilheus BA (Brazil). Grupo Bioenergia e Meio Ambiente]. E-mails: jalmeida@uesc.br; Parente Junior, Expedito [Tecnologias Bioenergeticas Ltda. (TECBIO), Fortaleza, CE (Brazil); Fundacao Nucleo de Tecnologia Industrial (NUTEC), Fortaleza, CE (Brazil)]. E-mail: expeditojr@tecbio.com.br

    2004-07-01

    Castor oil (Ricinus communis L.) is one of the cultures chosen by the biodiesel federal and state programs to supply raw material for biodiesel production - a biofuel indicated as renewable and less pollutant than its fossil competitor. An energetic balance was performed based on LCA - Life Cycle Analysis principles and Input-Output Analysis, comparing the castor oil energy performance with others traditional cultures: colza (Brassica napus) in Europe and soybean (Glycine max) in the United States. Energy balance (O-I) was positive in both production ways (methyl and ethyl) independent of coproduct use allocation alternative. The relation Output-Input (O-I) calculated for castor oil biodiesel [1.3-2.9] was higher than the colza (1.2-1.9) and lower than the soybean (3.2--3.4), independent of the way and allocation of the used byproduct. Both indicators suggest the energy and environmental viability of the castor oil biodiesel, provided that high agricultural productivity (higher than 1.500 kg/ha year) can be guaranteed. The potentialization of the positive energetic and environmental effects depends on the adequate utilization of the coproducts and process residues, the improvement of the energy efficiency in the the castor oil and biodiesel processing and the implementation of efficient management in the use of the chemical inputs (specially the N), responsible for up 5% of the total energy input.

  5. Pengaruh Konsentrasi Minyak Kelapa Murni (Virgin Coconut Oil dan Minyak Jarak (Castor Oil terhadap Sifat Fisikokimia dan Organoleptik Sabun Mandi Cair

    Directory of Open Access Journals (Sweden)

    Asri Widyasanti

    2017-04-01

    Full Text Available VCO contains of lauric acid that perform to smooth and to moisturize the skin. Castor oil has ricinoleic acid that serves to protect the skin. Therefore, both of the oil are suitable to be the raw material for liquid soap making. This study aimed were to produce a liquid soap, to determine the exact concentration of VCO and castor oil for liquid soap and to find out the effect of VCO and Castor Oil concentrations to the characteristics of liquid soap. The method used was laboratory experimental method with descriptive analysis. The treatments in this study were A =concentrations of VCO 100 %, B = concentrations of VCO 80 % and castor oil 20 %, C = concentrations of VCO 50 % and castor oil 50 %, D = concentrations of VCO 20 % and castor oil 80 %, and E = concentrations of castor oil 100 %,  from 200 gram soap base. The parameter observed for liquid soap included chemical properties, physical properties of soap, and organoleptic test. The result shows that all treatments complies the requirement SNI 06-4085-1996. The formula of liquid soap with treatment B was revealed as the best product with 0.01 % of total alkali content, pH value of 9.16 , specific gravity 1.06, and total plate count 5colonies/g. This technology process of natural liquid soap production with the VCO and castor oil could be develop and apply in industrial scale.

  6. Correlations between the anatomical traits of Gymnocladus canadensis Lam. in heartwood and sapwood of early- and latewood zones of growth rings

    OpenAIRE

    Jokanović Dušan; Vilotić Dragica; Mitrović Suzana; Miljković Danijela; Rebić Milan; Stanković Dragica; Nikolić Vesna

    2015-01-01

    This paper shows correlations between vessel characteristics and differences in growth-ring width in heartwood and sapwood. Analyzed samples were from an iron-wood tree (Gymnocladus canadensis Lam.) that grew in the Mužljanski Rit area, of the Srpska Crnja municipality in Serbia. According to previous research, it was deduced that Gymnocladus canadensis Lam. belongs to ring-porous species with big vessel lumen in the earlywood zone and thicker cell walls in...

  7. Comportamiento del bajo peso al nacer en la parroquia "Castor Nieves Ríos" Behavior of low birth weight in the "Castor Nieves Ríos" parish church

    Directory of Open Access Journals (Sweden)

    Yarelis González Pantoja

    2010-09-01

    Full Text Available INTRODUCCIÓN: el bajo peso al nacer constituye un problema multifactorial que afecta a muchos países del mundo. OBJETIVO: identificar los factores maternos que se relacionan con el bajo peso al nacer en la parroquia Castor Nieves Ríos. MÉTODOS: se realizó un estudio descriptivo transversal de los 32 nacimientos con bajo peso ocurridos en la maternidad del Hospital Público "José María Benítez", pertenecientes a la parroquia "Castor Nieves Ríos", en el 1er. semestre de 2006. Se revisaron las historias obstétricas de las madres, y se identificaron algunas variables sociodemográficas. RESULTADOS: el 90,62 % de los nacimientos con bajo peso correspondían a madres que fumaron durante la gestación. El 53,1 % de ellas, tuvo un parto pretérmino; el 40,62 % presentó anemia durante este período, y el 59,3 % de las gestantes tuvo una ganancia de peso insuficiente. CONCLUSIONES: el bajo peso al nacer estuvo determinado por el hábito de fumar, parto pretérmino, anemia, así como ganancia insuficiente de peso durante la gestación.INTRODUCTION: the low birth weight is multifactorial problem affecting many countries in the world. OBJECTIVE: to identify the maternal factors related to low birth weight in the "Castor Nieves Ríos" parish church. METHODS: a cross-sectional and descriptive study was conducted of 32 low birth weight births occurred in the "José María Benítez" Public Maternity Hospital during the 1st semester of 2006. The mother's obstetrical histories were reviewed and some sociodemographic variables were identified. RESULTS: the 90,62 % of low birth weight births corresponded to smoker mothers during pregnancy. The 53,1 % of them had a preterm labor; the 40,62 % had anemia during this period, and the 59,3 % of pregnants had a insufficient weight gain. CONCLUSIONS: the low birth weight was determined by smoking, preterm labor, anemia, as well as a insufficient weight gain during pregnancy.

  8. Síntesis y caracterización de espumas flexibles de poliuretano obtenidas a partir de aceite de castor maleinizado Flexible polyurethane foam synthesis and characterization obtained from maleinizated castor oil

    Directory of Open Access Journals (Sweden)

    Paula Mazo

    2009-06-01

    Full Text Available En este trabajo se realiza la síntesis de espumas flexibles de poliuretano empleando un diseño experimental Taguchi, utilizando aceite de castor con y sin maleinización, se estudia la cinética de la reacción de esterificación del aceite por cuantificación de los grupos ácido (ASTM D4662 - 03 y se evalúan las propiedades mecánicas de las espumas (densidad, resistencia tensil, % elongación, resiliencia y dureza. Norma NTC 2019 Icontec obtenidas en función de las variables de síntesis (cantidad y tipo de catalizador, relación molar aceite castor (CO - aceite maleinizado (MACO, cantidad de agua y surfactante empleado. Se realizan análisis de FTIR y SEM para las espumas. Los resultados mostraron una cinética de primer orden con respecto al anhídrido. El aceite maleinizado (MACO incrementa la resistencia mecánica de las espumas, debido al mayor entrecruzamiento por la incorporación de grupos ácido.In this work, the synthesis of polyurethane flexible foam is realized with the experimental design Taguchi, using castor oil with and without maleinization. The kinetic reaction of oil esterification was monitored by estimating the number of acid groups (ASTM D4662 - 03, and the mechanical properties of the foam were studied, including density, tensile resistance, % elongation, resilience and hardness (Norm NTC 2019 Icontec. The influence of various synthesis parameters was investigated, namely amount and type of catalyst, molar ratio of castor oil (CO - maleinizated castor oil (MACO, amount of water and surfactant used. The foams were analyzed using FTIR and scanning electron microscopy (SEM. The results showed first order kinetics with respect to the anhydride. The maleinizated castor oil (MACO increased the mechanical resistance of the foam due to the greater interweaving by the incorporation of acid groups.

  9. Management of spent fuel from power and research reactors using CASTOR and CONSTOR casks and licensing experience worldwide

    International Nuclear Information System (INIS)

    Becher, D.

    2003-01-01

    An overview of the spent fuel storage in CASTOR and CONSTOR casks during the last 30 years is made. Design characteristics of the both types of casks are presented. CASTOR casks fulfill both the requirements for type B packages according to the IAEA requirements covering different accident situations in storage sites. Analyses of nuclear and thermal behavior and strength are carried out for CONSTOR concept. Special experimental program for verification of mechanical and thermomechanical properties is implemented. Licensing experience of the casks in German storage facilities is presented. Special modifications of CASTOR casks for WWER-440 and RBMK fuel assemblies have been designed for implementation in Eastern Europe. Contracts for GNB spent fuel casks delivery are concluded with Czech Republic, Slovakia, Hungary and Lithuania

  10. Exogenous glutamine increases lipid accumulation in developing seeds of castor bean (Ricinus communis L. cultured in vitro

    Directory of Open Access Journals (Sweden)

    Zhang Yang

    2015-01-01

    Full Text Available This report describes biomass production and compositional changes of developing castor seeds in response to change in the nitrogen resource (glutamine of the medium. During the early developmental period (24-36 days after pollination, oil was found to initially accumulate in the developing seeds. Carbohydrates and oil were inversely related after glutamine provision (35 mM, in the culture medium. [U-14C] sucrose labeling was used to investigate the effect of metabolic fluxes among different storage materials. Addition of glutamine led to a 7% increase of labeling in lipids and an inverse decrease of labeling in carbohydrates. It was postulated that changes in the glutamine concentration in the medium are likely to influence the partitioning of resources between the various storage products, especially carbohydrates and oil. These observations will contribute to a better understanding of assimilate partitioning in developing castor seeds and the development of molecular strategies to improve castor bean seed quality and plant breeding studies.

  11. Comparative analysis of the anatomical structure of heartwood and sapwood selected Gymnocladus canadensis Lam. trees in Srpska Crnja

    Directory of Open Access Journals (Sweden)

    Vilotić Dragica

    2011-01-01

    Full Text Available This paper shows the results obtained from the study of the macroscopic-microscopic structure (capillary system in the growth stem of Gymnocladus canadensis Lam. originating from North America, which grows in “Muzljanski rit” in the area of Srpska Crnja. Gymnocladus canadensis Lam. falls under the ring-porous species according to its porosity, with large tracheas in its early zone. The early zone trachea lumens, contained in the sapwood, reach dimensions of up to 160 μm, while early zone trachea lumens in the growth stem rings of the sapwood reach dimensions of up to 120 μm. Examination of the microscopic structure of this tree show good properties of the tree.

  12. Alternatif Pembuatan Biodiesel Melalui Transesterifikasi Minyak Castor (Ricinus communis Menggunakan Katalis Campuran Cangkang Telur Ayam dan Kaolin

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    Soni - Setiadji

    2017-05-01

    Full Text Available Biodiesel was produced by transesterification of castor oil (Ricinus communis using a catalyst of CaO and kaolin (CaO / kaolin had been performed. CaO was obtained from the calcination of eggshell. Castor oil is selected as biodiesel feedstock because it belongs to non-food oil and easy to cultivate. In general, the research method aims to comprise the CaO / Kaolin catalysts with a ratio of 15 mmol CaO per 1 gram of kaolin activated using impregnation method and biodiesel produced through transesterification of castor oil using the catalyst at 65 ºC for 8 hours with ratio of castor oil: methanol: catalyst (1: 15: 5% w / w. The reaction is carried out on the reflux system. The XRD analysis show the presence of silica and potassium aluminum silicate hydroxide in the catalyst. The EDS results show the catalyst-forming components CaO and silica. The FTIR analysis results show the absorption peak in the functional group forming the methyl ester compound. Based on the characterization of GC-MS, the largest methyl ester components contained in biodiesel are methyl risinoleate, methyl elaidat, methyl stearate, methyl linoleate, and methyl palmitate. The overall conversion of castor oil to methyl ester using CaO / kaolin catalyst is 97.36%. The largest component in castor oil is risinoleic acid, has been successfully converted to methyl risinoleate by 74.75%.DOI: http://dx.doi.org/10.15408/jkv.v0i0.4778

  13. Decomposition of Phragmites australis litter retarded by invasive Solidago canadensis in mixtures: an antagonistic non-additive effect

    OpenAIRE

    Zhang, Ling; Zhang, Yaojun; Zou, Jianwen; Siemann, Evan

    2014-01-01

    Solidago canadensis is an aggressive invader in China. Solidago invasion success is partially attributed to allelopathic compounds release and more benefits from AM fungi, which potentially makes the properties of Solidago litter different from co-occurring natives. These properties may comprehensively affect litter decomposition of co-occurring natives. We conducted a field experiment to examine litter mixing effects in a Phragmites australis dominated community invaded by Solidago in southe...

  14. Cadmium uptake in Elodea canadensis leaves and its interference with extra- and intra-cellular pH.

    Science.gov (United States)

    Javed, M T; Lindberg, S; Greger, M

    2014-05-01

    This study investigated cadmium (Cd) uptake in Elodea canadensis shoots under different photosynthetic conditions, and its effects on internal (cytosolic) and external pH. The plants were grown under photosynthetic (light) or non-photosynthetic (dark or in the presence of a photosynthetic inhibitor) conditions in the presence or absence of CdCl2 (0.5 μm) in a medium with a starting pH of 5.0. The pH-sensitive dye BCECF-AM was used to monitor cytosolic pH changes in the leaves. Cadmium uptake in protoplasts and leaves was detected with a Cd-specific fluorescent dye, Leadmium Green AM, and with atomic absorption spectrophotometry. During cultivation for 3 days without Cd, shoots of E. canadensis increased the pH of the surrounding water, irrespective of the photosynthetic conditions. This medium alkalisation was higher in the presence of CdCl2 . Moreover, the presence of Cd also increased the cation exchange capacity of the shoots. The total Cd uptake by E. canadensis shoots was independent of photosynthetic conditions. Protoplasts from plants exposed to 0.5 μm CdCl2 for 3 days did not exhibit significant change in cytosolic [Cd(2+)] or pH. However, exposure to CdCl2 for 7 days resulted in increased cytosolic [Cd(2+) ] as well as pH. The results suggest that E. canadensis subjected to a low CdCl2 concentration initially sequesters Cd into the apoplasm, but under prolonged exposure, Cd is transported into the cytosol and subsequently alters cytosolic pH. In contrast, addition of 10-50 μm CdCl2 directly to protoplasts resulted in immediate uptake of Cd into the cytosol. © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands.

  15. Expansion for the Brachylophosaurus canadensis Collagen I Sequence and Additional Evidence of the Preservation of Cretaceous Protein.

    Science.gov (United States)

    Schroeter, Elena R; DeHart, Caroline J; Cleland, Timothy P; Zheng, Wenxia; Thomas, Paul M; Kelleher, Neil L; Bern, Marshall; Schweitzer, Mary H

    2017-02-03

    Sequence data from biomolecules such as DNA and proteins, which provide critical information for evolutionary studies, have been assumed to be forever outside the reach of dinosaur paleontology. Proteins, which are predicted to have greater longevity than DNA, have been recovered from two nonavian dinosaurs, but these results remain controversial. For proteomic data derived from extinct Mesozoic organisms to reach their greatest potential for investigating questions of phylogeny and paleobiology, it must be shown that peptide sequences can be reliably and reproducibly obtained from fossils and that fragmentary sequences for ancient proteins can be increasingly expanded. To test the hypothesis that peptides can be repeatedly detected and validated from fossil tissues many millions of years old, we applied updated extraction methodology, high-resolution mass spectrometry, and bioinformatics analyses on a Brachylophosaurus canadensis specimen (MOR 2598) from which collagen I peptides were recovered in 2009. We recovered eight peptide sequences of collagen I: two identical to peptides recovered in 2009 and six new peptides. Phylogenetic analyses place the recovered sequences within basal archosauria. When only the new sequences are considered, B. canadensis is grouped more closely to crocodylians, but when all sequences (current and those reported in 2009) are analyzed, B. canadensis is placed more closely to basal birds. The data robustly support the hypothesis of an endogenous origin for these peptides, confirm the idea that peptides can survive in specimens tens of millions of years old, and bolster the validity of the 2009 study. Furthermore, the new data expand the coverage of B. canadensis collagen I (a 33.6% increase in collagen I alpha 1 and 116.7% in alpha 2). Finally, this study demonstrates the importance of reexamining previously studied specimens with updated methods and instrumentation, as we obtained roughly the same amount of sequence data as the

  16. Analysis of active ricin and castor bean proteins in a ricin preparation, castor bean extract, and surface swabs from a public health investigation.

    Science.gov (United States)

    Schieltz, David M; McGrath, Sara C; McWilliams, Lisa G; Rees, Jon; Bowen, Michael D; Kools, John J; Dauphin, Leslie A; Gomez-Saladin, Eduardo; Newton, Bruce N; Stang, Heather L; Vick, Michael J; Thomas, Jerry; Pirkle, James L; Barr, John R

    2011-06-15

    In late February 2008, law enforcement officials in Las Vegas, Nevada, discovered in a hotel room, a copy of The Anarchist Cookbook, suspected castor beans and a "white powder" thought to be a preparation of ricin. Ricin is a deadly toxin from the seed of the castor bean plant (Ricinus communis). The United States regulates the possession, use, and transfer of ricin and it is the only substance considered a warfare agent in both the Chemical and the Biological Weapons Conventions. Six samples obtained from the hotel room were analyzed by laboratories at the Centers for Disease Control and Prevention using a panel of biological and mass spectrometric assays. The biological assays (real time-PCR, time resolved fluorescence and cytotoxicity) provided presumptive evidence of active ricin in each of the samples. This initial screen was followed by an in-depth analysis using a novel, state-of-the-art mass spectrometry-based ricin functional assay and high sensitivity tandem mass spectrometry for protein identification. Mass spectrometric analysis positively identified ricin and confirmed that in each of the samples it was enzymatically active. The tandem mass spectrometry analysis used here is the most selective method available to detect ricin toxin. In each sample, ricin was unequivocally identified along with other R. communis plant proteins, including the highly homologous protein RCA120. Although database searches using tandem mass spectra acquired from the samples indicated that additional controlled substances were not present in these samples, the mass spectrometric results did provide extensive detail about the sample contents. To the best of our knowledge following a review of the available literature, this report describes the most detailed analysis of a white powder for a public health or forensic investigation involving ricin. Published by Elsevier Ireland Ltd.

  17. Evaluation of reaction mechanisms and the kinetic parameters for the transesterification of castor oil by liquid enzymes

    DEFF Research Database (Denmark)

    Andrade, Thalles Allan; Errico, Massimiliano; Christensen, Knud Villy

    2017-01-01

    of the transesterification of castor oil with methanol using the enzyme Eversa® Transform as catalyst were investigated. Reactions were carried out for 8 hours at 35 °C with: an alcohol-to-oil molar ratio equal to 6:1, a 5 wt% of liquid enzyme solution and addition of 5 wt% of water by weight of castor oil. From...... methanolysis rates of glycerides obtained, indicated that transesterification dominates over hydrolysis. The mechanism among the four models proposed that gave the best fit could be simplified, eliminating the kinetic parameters with negligible effects on the reaction rates. This model was able to fit...

  18. Transesterification of castor oil usingMgO/SiO2 catalyst and coconutoilas co-reactant

    OpenAIRE

    Kamisah D. Pandiangan; Novesar Jamarun; Syukri Arief; Wasinton Simanjuntak

    2016-01-01

    This paper describes the transesterification of castor oil with the use of coconut oil as co-reactant and MgO/SiO2as heterogeneous base catalyst. The catalyst was preparedfrom rice husk silica and magnesium nitrate by sol-gel method, with MgO load of 20% relative to silica, and then subjected to sintering treatment at 600 oC for 6 hours. A series of experiments was carried out, indicating that the use of coconut oil as co-reactant significantly promoted the conversion of castor oil into b...

  19. Dry Cask Storage Characterization Project - Phase 1: CASTOR V/21 Cask Opening and Examination

    Energy Technology Data Exchange (ETDEWEB)

    Bare, Walter Claude; Ebner, Matthias Anthony; Torgerson, Laurence Dale

    2001-08-01

    This report documents visual examination and testing conducted in 1999 and early 2000 at the Idaho National Engineering and Environmental Laboratory (INEEL) on a Gesellschaft für Nuklear Service (GNS) CASTOR V/21 pressurized water reactor (PWR) spent fuel dry storage cask. The purpose of the examination and testing is to develop a technical basis for renewal of licenses and Certificates of Compliance for dry storage systems for spent nuclear fuel and high-level waste at independent spent fuel storage installation sites. The examination and testing was conducted to assess the condition of the cask internal and external surfaces, cask contents consisting of 21 Westinghouse PWR spent fuel assemblies from Dominion’s (formerly named Virginia Power) Surry Power Station and cask concrete storage pad. The assemblies have been continuously stored in the CASTOR cask since 1985. Cask exterior surface and selected fuel assembly temperatures, and cask surface gamma and neutron dose rates were measured. Cask external/internal surfaces, fuel basket components including accessible weldments, fuel assembly exteriors, and primary lid seals were visually examined. Selected fuel rods were removed from one fuel assembly, visually examined, and then shipped to Argonne National Laboratory for nondestructive, destructive, and mechanical examination. Cask interior crud samples and helium cover gas samples were collected and analyzed. The results of the examination and testing indicate the concrete storage pad, CASTOR V/21 cask, and cask contents exhibited sound structural and seal integrity and that long-term storage has not caused detectable degradation of the spent fuel cladding or the release of gaseous fission products between 1985 and 1999.

  20. Optimization of Mechanical Expression of Castor Seeds Oil (Ricinus communis using Response Surface Methodology

    Directory of Open Access Journals (Sweden)

    J. O. Olaoye

    2017-12-01

    Full Text Available The effect of the processing parameters of Castor seed on its oil yield was investigated. The castor seeds were passed through drying, crushing and separation into seeds and shells. These processing conditions were further succeeded by seed roasting and subsequent mechanical expression of the roasted nut by means of screw press in the course of its preparation for oil expression. Seed samples were conditioned by adding calculated amount of distilled water to obtain different moisture levels from the initial moisture content of the seeds. Samples were roasted at the temperatures of 83.18, 90.00, 100.00, 110.00 and 116.82°C, over periods of 6.59, 10.00, 15.00, 20.00 and 23.41min, seed moisture content of 6.32, 7.00, 8.00, 9.00 and 9.68 % wb, respectively and the oil was expressed using a screw roaster-expeller. Optimization of the oil expression process was achieved by applying Central Composite Rotatable Design of Response Surface Methodology. The optimal conditions for oil yield within the experimental range of the studied variables were 7%, 110°C and 20 min; moisture content, roasting temperature and roasting duration respectively. These values of the optimum process conditions were used to predict optimum value of oil yield to be 25.77%. A second-order model was obtained to predict oil yield as a function of moisture content, heating temperature and duration. Thus the result from this research work has established the optimal conditions for mechanical extraction of oil from castor seed. Closed agreement between experimental and predicted yield was obtained.

  1. Collection of castor-oil plant germoplasm (Ricinus communis L. in two municipalities of Arauca, Colombia

    Directory of Open Access Journals (Sweden)

    Carlos Iván Cardozo Conde

    2013-05-01

    Full Text Available Ricinus communis L., commonly known as the castor-oil plant, is important for its use in biofuels production. With the objective of learning about its current status, in 40 villages influential to the oil complex of Caricare (municipality of Arauquita and Caño Limón (municipality of Arauca in Arauca, Colombia (where five years earlier the crop had been established, a collection of sexual seed was carried out between December 2011 and January 2012. The variables studied include passport data as a collection resource, local name, relief, and soil type among others. A Garmin map76CSx was used in order to identify the transects and record data such as geographic location and elevation. Simple descriptive statistics were used to identify the variables of greatest variation. Using the qualitative variables of greatest importance, a contingency table analysis with a significance level of 5%, a multiple correspondence analysis (MCA and principal component analysis (PCA were performed for quantitative and qualitative variables, in addition to a classification analysis through a similarity matrix. The castor-oil plant was found in 25% of the villages visited. 12 introductions were collected, four from Caricare and eight from Caño Limón. Although the environmental conditions were favo­rable for its cultivation, there are no castor-oil plant crops in the locations visited. The absence of grain commercialization and oil extraction equipment is the main limiting factor. A garden was established using collected materials for the purpose of research, breeding and propagation.

  2. Reproductive responses and productive characteristics in ewes supplemented with detoxified castor meal for a long period

    Directory of Open Access Journals (Sweden)

    Liliane Moreira Silva

    2014-08-01

    Full Text Available The objective of the present study was to evaluate the effects of supplementation with detoxified castor meal (DCM in the diet of ewes during pregnancy, partum, and post-partum on the weight development of their offspring and at slaughter. The study included 56 ewes with synchronized estrus that were naturally mated. At the beginning of pregnancy and in post-partum, hepatic and renal function-related parameters and progesterone levels were measured. At slaughter, the proximate composition and fatty acid profile were determined in the loin of ewes. There was no effect of diet on reproductive response after estrus synchronization. At the beginning of pregnancy, albumin and creatinine levels were lower in the DCM group. Supplementation with DCM did not alter the weight or body condition of ewes at partum. However, at weaning, the DCM group showed a higher loin-eye area (LEA in relation to the group fed diets without detoxified castor meal (WDCM. At partum, as well as at weaning, the offspring of the ewes supplemented with DCM had a larger LEA than the WDCM group. In post-partum, levels of glucose, urea, protein, and cholesterol were lower in the DCM group. The return to cyclicity was similar in both groups, with an average of 47 days after partum. At slaughter, neither anatomical and carcass components nor the results of the proximate analysis were affected by the type of diet, except for an increase in heptadecanoic acid in the DCM group. Supplementation with detoxified castor meal in the diet of ewes does not affect lambing, pregnancy, prolificacy, return to cyclicity, milk production, blood biochemical parameters, or carcass characteristics.

  3. Glutathione preservation during storage of rat lenses in optisol-GS and castor oil.

    Science.gov (United States)

    Holm, Thomas; Brøgger-Jensen, Martin Rocho; Johnson, Leif; Kessel, Line

    2013-01-01

    Glutathione concentration in the lens decreases in aging and cataractous lenses, providing a marker for tissue condition. Experimental procedures requiring unfrozen lenses from donor banks rely on transportation in storage medium, affecting lens homeostasis and alterations in glutathione levels. The aim of the study was to examine the effects of Optisol-GS and castor oil on lens condition, determined from their ability to maintain glutathione concentrations. Rat lenses were stored in the two types of storage media at varying time intervals up to 3 days. Glutathione concentration was afterwards determined in an enzymatic detection assay, specific for both reduced and oxidized forms. Lenses removed immediately after death exhibited a glutathione concentration of 4.70±0.29 mM. In vitro stored lenses in Optisol-GS lost glutathione quickly, ending with a concentration of 0.60±0.34 mM after 3 days while castor oil stored lenses exhibited a slower decline and ended at 3 times the concentration. A group of lenses were additionally stored under post mortem conditions within the host for 6 hours before its removal. Total glutathione after 6 hours was similar to that of lenses removed immediately after death, but with altered GSH and GSSG concentrations. Subsequent storage of these lenses in media showed changes similar to those in the first series of experiments, albeit to a lesser degree. It was determined that storage in Optisol-GS resulted in a higher loss of glutathione than lenses stored in castor oil. Storage for more than 12 hours reduced glutathione to half its original concentration, and was considered unusable after 24 hours.

  4. Microbial degradation, cytotoxicity and antibacterial activity of polyurethanes based on modified castor oil and polycaprolactone.

    Science.gov (United States)

    Uscátegui, Yomaira L; Arévalo, Fabián R; Díaz, Luis E; Cobo, Martha I; Valero, Manuel F

    2016-10-11

    The objective of this study was to assess the effects of type of polyol and concentration of polycaprolactone (PCL) in polyurethanes (PUs) on microbial degradability, cytotoxicity, biological properties and antibacterial activity to establish whether these materials may have biomedical applications. Chemically modified and unmodified castor oil, PCL and isophorone diisocyanate in a 1:1 ratio of NCO/OH were used. PUs were characterized by stress/strain fracture tests and hardness (ASTM D 676-59). Hydrophilic character was determined by contact angle trials and morphology was evaluated by scanning electron microscopy. Degradability with Escherichia coli and Pseudomonas aeruginosa was evaluated by measuring variations in the weight of the polymers. Cytotoxicity was evaluated using the ISO 10993-5 (MTT) method with mouse embryonic fibroblasts L-929 (ATCC® CCL-1) in direct contact with the PUs and with NIH/3T3 cells (ATCC® CRL-1658) in indirect contact with the PUs. Antimicrobial activity against E. coli and P. aeruginosa was determined. PUs derived from castor oil modified (P0 and P1) have higher mechanical properties than PUs obtained from castor oil unmodified (CO). The viability of L-929 mouse fibroblasts in contact with polymers was greater than 70%. An assessment of NIH/3T3 cells in indirect contact with PUs revealed no-toxic degradation products. Finally, the antibacterial effect of the PUs decreased by 77% for E. coli and 56% for P. aeruginosa after 24 h. These results indicate that PUs synthesized with PCL have biocidal activity against Gram-negative bacteria and do not induce cytotoxic responses, indicating the potential use of these materials in the biomedical field.

  5. Glutathione preservation during storage of rat lenses in optisol-GS and castor oil.

    Directory of Open Access Journals (Sweden)

    Thomas Holm

    Full Text Available BACKGROUND: Glutathione concentration in the lens decreases in aging and cataractous lenses, providing a marker for tissue condition. Experimental procedures requiring unfrozen lenses from donor banks rely on transportation in storage medium, affecting lens homeostasis and alterations in glutathione levels. The aim of the study was to examine the effects of Optisol-GS and castor oil on lens condition, determined from their ability to maintain glutathione concentrations. METHODOLOGY/PRINCIPAL FINDINGS: Rat lenses were stored in the two types of storage media at varying time intervals up to 3 days. Glutathione concentration was afterwards determined in an enzymatic detection assay, specific for both reduced and oxidized forms. Lenses removed immediately after death exhibited a glutathione concentration of 4.70±0.29 mM. In vitro stored lenses in Optisol-GS lost glutathione quickly, ending with a concentration of 0.60±0.34 mM after 3 days while castor oil stored lenses exhibited a slower decline and ended at 3 times the concentration. A group of lenses were additionally stored under post mortem conditions within the host for 6 hours before its removal. Total glutathione after 6 hours was similar to that of lenses removed immediately after death, but with altered GSH and GSSG concentrations. Subsequent storage of these lenses in media showed changes similar to those in the first series of experiments, albeit to a lesser degree. CONCLUSIONS/SIGNIFICANCE: It was determined that storage in Optisol-GS resulted in a higher loss of glutathione than lenses stored in castor oil. Storage for more than 12 hours reduced glutathione to half its original concentration, and was considered unusable after 24 hours.

  6. Local sustained delivery of bupivacaine HCl from a new castor oil-based nanoemulsion system.

    Science.gov (United States)

    Rachmawati, Heni; Arvin, Yang Aryani; Asyarie, Sukmadjaja; Anggadiredja, Kusnandar; Tjandrawinata, Raymond Rubianto; Storm, Gert

    2018-06-01

    Bupivacaine HCl (1-butyl-2',6'-pipecoloxylidide hydrochloride), an amide local anesthetic compound, is a local anesthetic drug utilized for intraoperative local anesthesia, post-operative analgesia and in the treatment of chronic pain. However, its utility is limited by the relative short duration of analgesia after local administration (approximately 9 h after direct injection) and risk for side effects. This work is aimed to develop a nanoemulsion of bupivacaine HCl with sustained local anesthetics release kinetics for improved pain management, by exhibiting extended analgesic action and providing reduced peak levels in the circulation to minimize side effects. Herein, biodegradable oils were evaluated for use in nanoemulsions to enable sustained release kinetics of bupivacaine HCl. Only with castor oil, a clear and stable nanoemulsion was obtained without the occurrence of phase separation over a period of 3 months. High loading of bupivacaine HCl into the castor oil-based nanoemulsion system was achieved with about 98% entrapment efficiency and the resulting formulation showed high stability under stress conditions (accelerated stability test) regarding changes in visual appearance, drug content, and droplet size. We show herein that the in vitro release and in vivo pharmacokinetic profiles as well as pharmacodynamic outcome (pain relief test) after subcutaneous administration in rats correlate well and clearly demonstrate the prolonged release and extended duration of activity of our novel nanoformulation. In addition, the lower C max value achieved in the blood compartment suggests the possibility that the risk for systemic side effects is reduced. We conclude that castor oil-based nanomulsion represents an attractive pain treatment possibility to achieve prolonged local action of bupivacaine HCl.

  7. Lipase applications in oil hydrolysis with a case study on castor oil: a review.

    Science.gov (United States)

    Goswami, Debajyoti; Basu, Jayanta Kumar; De, Sirshendu

    2013-03-01

    Lipase (triacylglycerol acylhydrolase) is a unique enzyme which can catalyze various types of reactions such as hydrolysis, esterification, alcoholysis etc. In particular, hydrolysis of vegetable oil with lipase as a catalyst is widely studied. Free lipase, lipase immobilized on suitable support, lipase encapsulated in a reverse micelle and lipase immobilized on a suitable membrane to be used in membrane reactor are the most common ways of employing lipase in oil hydrolysis. Castor oil is a unique vegetable oil as it contains high amounts (90%) of a hydroxy monounsaturated fatty acid named ricinoleic acid. This industrially important acid can be obtained by hydrolysis of castor oil. Different conventional hydrolysis processes have certain disadvantages which can be avoided by a lipase-catalyzed process. The degree of hydrolysis varies widely for different lipases depending on the operating range of process variables such as temperature, pH and enzyme loading. Immobilization of lipase on a suitable support can enhance hydrolysis by suppressing thermal inactivation and estolide formation. The presence of metal ions also affects lipase-catalyzed hydrolysis of castor oil. Even a particular ion has different effects on the activity of different lipases. Hydrophobic organic solvents perform better than hydrophilic solvents during the reaction. Sonication considerably increases hydrolysis in case of lipolase. The effects of additives on the same lipase vary with their types. Nonionic surfactants enhance hydrolysis whereas cationic and anionic surfactants decrease it. A single variable optimization method is used to obtain optimum conditions. In order to eliminate its disadvantages, a statistical optimization method is used in recent studies. Statistical optimization shows that interactions between any two of the following pH, enzyme concentration and buffer concentration become significant in presence of a nonionic surfactant named Span 80.

  8. Test of 10 GHz sin-cosin microwave reflectometer on CASTOR

    International Nuclear Information System (INIS)

    Zacek, F.; Kletecka, P.

    1994-09-01

    The first microwave reflectometric device is described used at the CASTOR tokamak to measure fast density fluctuations. The device operates at the frequency of 10.26 GHz which makes it possible to detect fluctuations near the plasma periphery. The device was proved to work properly during the whole tokamak discharge despite the fact that the reflected signal level varied strongly. The construction of the reflectometric device is described as is its use of the so-called sin-cosin detection system, and the results obtained are discussed. (Z.S.) 8 figs., 3 refs

  9. Castor oil polyurethane as a coating option for spent nuclear fuel disposal containment

    Energy Technology Data Exchange (ETDEWEB)

    Mortley, A.; Bonin, H.W.; Bui, V.T. [Dept. of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario (Canada)

    2009-07-01

    Castor oil polyurethane (COPU) coatings are being proposed as an additional barrier in the design of the copper containers to store spent nuclear fuel in Canada. The present work investigates the variation in the physicomechanical properties of two COPUs, based on an aliphatic and aromatic diisocyanate, as a function of ionizing radiation dose and dose rate. The changes in physicomechanical properties have shown that radiation, regardless of dose rate and isocyanate structure, increases the values of the modulus and the ultimate tensile strength when compared with those of the unirradiated samples, with aromatic based polyurethanes being more susceptible to variation than aliphatic based ones. (author)

  10. Meat quality assessment from young goats fed for long periods with castor de-oiled cake.

    Science.gov (United States)

    Oliveira, C H A; Silva, A M; Silva, L M; van Tilburg, M F; Fernandes, C C L; Moura, A A; Moreno, F B M B; Monteiro-Moreira, A C O; Moreira, R A; Bezerra, F J; Rondina, D

    2015-08-01

    Diet can influence both the qualitative and quantitative traits of ruminant meat. This study evaluated the effects of castor de-oiled cake on the meat of mixed-breed male goat kids. After 165days of diet treatment, no alterations (p>0.05) were observed in the in vivo performance, anatomic components, dissection and proximate composition of the Longissimus dorsi muscle, as well as in the color and pH of the carcasses. However, diet had an effect (pgoats led to alterations in meat quality, without compromising its consumption qualities. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Evaluation gamma radiation in composite sisal fiber- polyurethane derived of castor oil by bending test

    International Nuclear Information System (INIS)

    Souza, Felipe H. de; Geraldo, Ricardo R.; Vasco, Marina C.; Azevedo, Elaine; Claro Neto, Salvador

    2015-01-01

    Materials used for making furniture and accessories or positioning in X -ray examination rooms should not exhale volatile organic compounds and are resistant to ionizing radiation. One solution is the use of vegetable fiber and polyurethane composites of vegetable origin, since they are biodegradable, derived from renewable raw materials and have no volatile organic compounds. The main difficulty in developing this material is fiber adhesion with the polymer. The objective of this study is to evaluate the mechanical properties of composite sisal fiber composite, without further treatment, and polyurethane derived from castor oil, with a dose of 25 kGy gamma radiation, subjected to 3 points bending tests. (author)

  12. Aluminum and steel adhesion with polyurethanes from castor oil adhesives submitted to gamma irradiation

    International Nuclear Information System (INIS)

    Azevedo, Elaine C.; Assumpcao, Roberto L.; Nascimento, Eduardo M. do; Claro Neto, Salvador; Soboll, Daniel S.

    2009-01-01

    Polyurethanes adhesive from castor oil is used to join aluminum and steel pieces. The effect of gamma radiation on the resistance to tension tests is investigated. The aluminum and steel pieces after being glued with the adhesive were submitted to gamma irradiation in doses of 1 kGy, 25 kGy and 100 kGy. The rupture strength of the joints after irradiation have a slightly increase or remains practically unchanged indicating that the adhesive properties is not affected by the gamma radiation. (author)

  13. Study of very forward jets at 13 TeV with the CASTOR calorimeter of CMS

    Energy Technology Data Exchange (ETDEWEB)

    Baur, Sebastian; Akbiyik, Melike; Baus, Colin; Katkov, Igor; Ulrich, Ralf; Woehrmann, Hauke [Institut fuer Experimentelle Kernphysik, Karlsruher Institut fuer Technologie (Germany)

    2015-07-01

    The CASTOR calorimeter of CMS measures QCD jets at pseudorapidities -5.2 ≥ η ≥ -6.6. Due to this unique very forward acceptance, such data is very discriminating for hadronic event generators. In particular since values of Bjorken-x down to 10{sup -6} are probed. In preparation for the upcoming LHC Run 2 at √(s) = 13 TeV, a Monte Carlo study of such jets is presented, including full detector simulation with GEANT4. We investigate methods of data- and Monte Carlo-driven jet energy corrections. First results of p{sub t}-balancing and detector unfolding are presented.

  14. Evaluation of the properties of iron oxide-filled castor oil polyurethane

    OpenAIRE

    Mussatti, Eleonora; Merlini, Claudia; Barra, Guilherme Mariz de Oliveira; Güths, Saulo; Oliveira, Antonio Pedro Novaes de; Siligardi, Cristina

    2012-01-01

    The aim of this study was to obtain and evaluate the electrical, thermal and mechanical properties of iron oxide-filled castor oil polyurethane (PU/Fe2O3). The iron oxide used in this study was a residue derived from the steel pickling process of a Brazilian steel rolling industry. Polymeric composites with different iron oxide volume fractions (2.5, 5.0, 7.5, 10.0 and 12.5%) were prepared through the casting process followed by compression molding at room temperature. The composites were ana...

  15. Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius

    Science.gov (United States)

    Narwal, Sunil Kumar; Saun, Nitin Kumar; Dogra, Priyanka; Chauhan, Ghanshyam

    2015-01-01

    A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, 1H NMR spectra, and gas chromatography. PMID:25874205

  16. Castor oil polyurethane as a coating option for spent nuclear fuel disposal containment

    International Nuclear Information System (INIS)

    Mortley, A.; Bonin, H.W.; Bui, V.T.

    2009-01-01

    Castor oil polyurethane (COPU) coatings are being proposed as an additional barrier in the design of the copper containers to store spent nuclear fuel in Canada. The present work investigates the variation in the physicomechanical properties of two COPUs, based on an aliphatic and aromatic diisocyanate, as a function of ionizing radiation dose and dose rate. The changes in physicomechanical properties have shown that radiation, regardless of dose rate and isocyanate structure, increases the values of the modulus and the ultimate tensile strength when compared with those of the unirradiated samples, with aromatic based polyurethanes being more susceptible to variation than aliphatic based ones. (author)

  17. Yield of castor bean fertilized with sewage sludge and potassium and magnesium sulphate

    OpenAIRE

    Cavalcanti,Thâmara F. M.; Zuba,Geraldo R.; Sampaio,Regynaldo A.; Carneiro,João P.; Oliveira,Ely S. A. de; Rodrigues,Márcio N.

    2015-01-01

    ABSTRACTThe aim of this study was to evaluate the yield and nutrition of castor bean in response to fertilization with sewage sludge and potassium (K) and magnesium (Mg) sulphate. The experiment was carried out from January to July 2011. The treatments, in a randomized block design with three replicates, in a Nitosol, corresponded to a factorial scheme (2 x 4 +1): two doses of K and Mg sulphate combined with four doses of sewage sludge (0, 2.60, 5.20 and 10.40 t ha-1, dry basis), applied base...

  18. First performance studies of a prototype for the CASTOR forward calorimeter at the CMS experiment

    OpenAIRE

    Aslanoglou, Xenofon; Cyz, Antoni; Davis, N; D'Enterria, David; Gladysz-Dziadus, Ewa; Kalfas, Costas; Musienko, Yuri; Kuznetsov, Andrey; Panagiotou, Apostolos

    2008-01-01

    We present results on the performance of the first prototype of the CASTOR quartz-tungsten sampling calorimeter, to be installed in the very forward region of the CMS experiment at the LHC. This study includes GEANT Monte Carlo simulations of the Cherenkov light transmission efficiency of different types of air-core light guides, as well as analysis of the calorimeter linearity and resolution as a function of energy and impact-point, obtained with 20-200 GeV electron beams from CERN/SPS tests...

  19. Synthesis of colloids based on gold nanoparticles dispersed in castor oil

    International Nuclear Information System (INIS)

    Silva, E. C. da; Silva, M. G. A. da; Meneghetti, S. M. P.; Machado, G.; Alencar, M. A. R. C.; Hickmann, J. M.; Meneghetti, M. R.

    2008-01-01

    New colloidal solutions of gold nanoparticles (AuNP), using castor oil as a nontoxic organic dispersant agent, were prepared via three different methods. In all three cases, tetrachloroauric(III) acid was employed as the gold source. The colloids were characterized by UV-Vis spectroscopy and transmission electron microscopy (TEM). The AuNP produced by the three methods were quasispherical in shape, however with different average sizes. The individual characteristics of the nanoparticles presented in each colloidal system were also confirmed by observation of absorption maxima at different wavelengths of visible light. Each method of synthesis leads to colloids with different grades of stability with respect to particle agglomeration.

  20. Apis mellifera pollination improves agronomic productivity of anemophilous castor bean (Ricinus communis

    Directory of Open Access Journals (Sweden)

    Rômulo A.G. Rizzardo

    2012-12-01

    Full Text Available Castor bean (Ricinus communis L. is cultivated mainly for biodiesel production because of its oil-rich seeds; it is assumed to be an anemophylous species. But pollination deficit can lead to low productivity often attributed to other reasons. In this paper, we investigated pollination requirements, pollination mechanism, occurrence of pollination deficit, and the role of biotic pollinators in a large commercial plantation of castor bean. Our results show that R. communis bears a mixed breeding system favoring selfing by geitonogamy, although the wind promotes mostly outcrossing. We also found that the honey bee (Apis mellifera L. foraging on castor bean can both transfer pollen from male to female flowers within the same raceme and boost the release of airborne pollen by male flowers. Both situations increase geitonogamy rates, raising significantly fruit set and seed yield. This is the first report of an animal foraging activity increasing seed yield in an anemophilous and geitonogamous crop and elucidates the role of biotic pollinators in castor bean reproduction.A mamoneira (Ricinus communis L. é cultivada principalmente para produção de biodiesel devido ao alto teor de óleo de suas sementes e considerada como sendo de polinização anemófila. Mas déficits de polinização podem levar a baixos índices de produtividade geralmente atribuídos a outros fatores. Neste trabalho foram investigados os requerimentos, mecanismos e déficit de polinização e o papel dos polinizadores bióticos em um monocultivo comercial de mamona. Os resultados mostram que R. communis possui um sistema de polinização misto, favorecendo a autopolinização por geitonogamia, embora o vento normalmente promova polinização cruzada. Observou-se também que a abelha melífera (Apis mellifera L. forrageando na mamoneira pode tanto transferir pólen das flores estaminadas para as pistiladas do mesmo racemo, quanto aumentar consideravelmente a liberação de p

  1. Characterisation of Antigen B Protein Species Present in the Hydatid Cyst Fluid of Echinococcus canadensis G7 Genotype.

    Science.gov (United States)

    Folle, Ana Maite; Kitano, Eduardo S; Lima, Analía; Gil, Magdalena; Cucher, Marcela; Mourglia-Ettlin, Gustavo; Iwai, Leo K; Rosenzvit, Mara; Batthyány, Carlos; Ferreira, Ana María

    2017-01-01

    The larva of cestodes belonging to the Echinococcus granulosus sensu lato (s.l.) complex causes cystic echinococcosis (CE). It is a globally distributed zoonosis with significant economic and public health impact. The most immunogenic and specific Echinococcus-genus antigen for human CE diagnosis is antigen B (AgB), an abundant lipoprotein of the hydatid cyst fluid (HF). The AgB protein moiety (apolipoprotein) is encoded by five genes (AgB1-AgB5), which generate mature 8 kDa proteins (AgB8/1-AgB8/5). These genes seem to be differentially expressed among Echinococcus species. Since AgB immunogenicity lies on its protein moiety, differences in AgB expression within E. granulosus s.l. complex might have diagnostic and epidemiological relevance for discriminating the contribution of distinct species to human CE. Interestingly, AgB2 was proposed as a pseudogene in E. canadensis, which is the second most common cause of human CE, but proteomic studies for verifying it have not been performed yet. Herein, we analysed the protein and lipid composition of AgB obtained from fertile HF of swine origin (E. canadensis G7 genotype). AgB apolipoproteins were identified and quantified using mass spectrometry tools. Results showed that AgB8/1 was the major protein component, representing 71% of total AgB apolipoproteins, followed by AgB8/4 (15.5%), AgB8/3 (13.2%) and AgB8/5 (0.3%). AgB8/2 was not detected. As a methodological control, a parallel analysis detected all AgB apolipoproteins in bovine fertile HF (G1/3/5 genotypes). Overall, E. canadensis AgB comprised mostly AgB8/1 together with a heterogeneous mixture of lipids, and AgB8/2 was not detected despite using high sensitivity proteomic techniques. This endorses genomic data supporting that AgB2 behaves as a pseudogene in G7 genotype. Since recombinant AgB8/2 has been found to be diagnostically valuable for human CE, our findings indicate that its use as antigen in immunoassays could contribute to false negative results in

  2. Characterisation of Antigen B Protein Species Present in the Hydatid Cyst Fluid of Echinococcus canadensis G7 Genotype

    Science.gov (United States)

    Folle, Ana Maite; Kitano, Eduardo S.; Lima, Analía; Gil, Magdalena; Cucher, Marcela; Mourglia-Ettlin, Gustavo; Iwai, Leo K.; Rosenzvit, Mara; Batthyány, Carlos

    2017-01-01

    The larva of cestodes belonging to the Echinococcus granulosus sensu lato (s.l.) complex causes cystic echinococcosis (CE). It is a globally distributed zoonosis with significant economic and public health impact. The most immunogenic and specific Echinococcus-genus antigen for human CE diagnosis is antigen B (AgB), an abundant lipoprotein of the hydatid cyst fluid (HF). The AgB protein moiety (apolipoprotein) is encoded by five genes (AgB1-AgB5), which generate mature 8 kDa proteins (AgB8/1-AgB8/5). These genes seem to be differentially expressed among Echinococcus species. Since AgB immunogenicity lies on its protein moiety, differences in AgB expression within E. granulosus s.l. complex might have diagnostic and epidemiological relevance for discriminating the contribution of distinct species to human CE. Interestingly, AgB2 was proposed as a pseudogene in E. canadensis, which is the second most common cause of human CE, but proteomic studies for verifying it have not been performed yet. Herein, we analysed the protein and lipid composition of AgB obtained from fertile HF of swine origin (E. canadensis G7 genotype). AgB apolipoproteins were identified and quantified using mass spectrometry tools. Results showed that AgB8/1 was the major protein component, representing 71% of total AgB apolipoproteins, followed by AgB8/4 (15.5%), AgB8/3 (13.2%) and AgB8/5 (0.3%). AgB8/2 was not detected. As a methodological control, a parallel analysis detected all AgB apolipoproteins in bovine fertile HF (G1/3/5 genotypes). Overall, E. canadensis AgB comprised mostly AgB8/1 together with a heterogeneous mixture of lipids, and AgB8/2 was not detected despite using high sensitivity proteomic techniques. This endorses genomic data supporting that AgB2 behaves as a pseudogene in G7 genotype. Since recombinant AgB8/2 has been found to be diagnostically valuable for human CE, our findings indicate that its use as antigen in immunoassays could contribute to false negative results in

  3. Pulmonary adiaspiromycosis in the Eurasian beaver (Castor fiber) inhabiting Poland.

    Science.gov (United States)

    Dolka, I; Giżejewska, A; Giżejewski, Z; Kołodziejska-Lesisz, J; Kluciński, W

    2017-09-26

    Adiaspiromycosis is a rare fungal infection caused by saprophytic fungi Emmonsia spp. (type Ascomycota) occurring especially in small free-living mammals. The aim of this study was to evaluate the occurrence of histopathological lesions asscociated with adiaspiromycosis in the Eurasian beaver inhabiting Poland. In order to evaluate the presence of natural adiaspiromycosis we systematically investigated beaver populations from north-eastern Poland for adiaspores in the lungs. This study reveals for the first time the presence of pulmonary adiaspiromycosis of Eurasian beaver in Poland. As far as we know, there is no published data regarding pulmonary adiaspiromycosis in human patients in Poland.

  4. The first report of human-derived G10 genotype of Echinococcus canadensis in China and possible sources and routes of transmission.

    Science.gov (United States)

    Yang, Dong; Zhang, Tiemin; Zeng, Zhaolin; Zhao, Wei; Zhang, Weizhe; Liu, Aiqin

    2015-10-01

    Cystic echinococcosis (CE) is one of the most important parasitic zoonoses. 10 distinct genotypes, designated G1-G10 genotypes of Echinococcus granulosus sensu lato (s.l.), have been split into 4 species: Echinococcus granulosus sensu stricto (s.s.) (G1-G3), Echinococcus equinus (G4), Echinococcus ortleppi (G5) and Echinococcus canadensis (G6-G10); Echinococcus felidis has also been suggested as a sister taxon of E. granulosus s.s. recently. Four genotypes belonging to two species (G1 and G3 genotypes of E. granulosus s.s., and G6 and G7 genotypes of E. canadensis) have been identified in humans and animals in China. In the present study, a human-derived hydatid cyst from a patient in northeastern China's Heilongjiang Province was identified as G10 genotype of E. canadensis based on mitochondrial cytochrome c oxidase subunit I (cox1), cytochrome b (cytb) and NADH dehydrogenase subunit 1 (nad1) genes. Homology analysis showed the cox1 gene sequence of G10 genotype of E. canadensis had 100% homology with those from wolves in Mongolia and from a moose in Russia. The cytb and nad1 gene sequences of G10 genotype of E. canadensis had 100% homology with the complete sequence from a moose in Finland at an amino acid level. The infection source of the CE patient here might be primarily attributable to wolves. This is the first report of G10 genotype of E. canadensis in a human in China. The finding of G10 genotype of E. canadensis in China shows that this genotype possibly has a more wide geographical distribution than previously considered. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  5. Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination.

    Science.gov (United States)

    Zhang, Hong; Zhou, Ke-Xin; Wang, Wei-Qing; Liu, Shu-Jun; Song, Song-Quan

    2017-06-01

    Poplar (Populus×canadensis) seeds rapidly germinated in darkness at 10, 15, and 20°C and reached 50% seed germination after about 22, 4.5, and 3.5h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50μM and cycloheximide (CHX) at 100μM, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, Pgermination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins. Copyright © 2017 Elsevier GmbH. All rights reserved.

  6. Evidence for enhanced mutualism hypothesis: Solidago canadensis plants from regular soils perform better.

    Science.gov (United States)

    Sun, Zhen-Kai; He, Wei-Ming

    2010-11-03

    The important roles of plant-soil microbe interactions have been documented in exotic plant invasion, but we know very little about how soil mutualists enhance this process (i.e. enhanced mutualism hypothesis). To test this hypothesis we conducted two greenhouse experiments with Solidago canadensis (hereafter Solidago), an invasive forb from North America, and Stipa bungeana (hereafter Stipa), a native Chinese grass. In a germination experiment, we found soil microbes from the rhizospheres of Solidago and Stipa exhibited much stronger facilitative effects on emergence of Solidago than that of Stipa. In a growth and competition experiment, we found that soil microbes strongly facilitated Solidago to outgrow Stipa, and greatly increased the competitive effects of Solidago on Stipa but decreased the competitive effects of Stipa on Solidago. These findings from two experiments suggest that in situ soil microbes enhance the recruitment potential of Solidago and its ability to outcompete native plants, thereby providing strong evidence for the enhanced mutualism hypothesis. On the other hand, to some extent this outperformance of Solidago in the presence of soil microbes seems to be unbeneficial to control its rapid expansion, particularly in some ranges where this enhanced mutualism dominates over other mechanisms.

  7. Evidence for enhanced mutualism hypothesis: Solidago canadensis plants from regular soils perform better.

    Directory of Open Access Journals (Sweden)

    Zhen-Kai Sun

    Full Text Available The important roles of plant-soil microbe interactions have been documented in exotic plant invasion, but we know very little about how soil mutualists enhance this process (i.e. enhanced mutualism hypothesis. To test this hypothesis we conducted two greenhouse experiments with Solidago canadensis (hereafter Solidago, an invasive forb from North America, and Stipa bungeana (hereafter Stipa, a native Chinese grass. In a germination experiment, we found soil microbes from the rhizospheres of Solidago and Stipa exhibited much stronger facilitative effects on emergence of Solidago than that of Stipa. In a growth and competition experiment, we found that soil microbes strongly facilitated Solidago to outgrow Stipa, and greatly increased the competitive effects of Solidago on Stipa but decreased the competitive effects of Stipa on Solidago. These findings from two experiments suggest that in situ soil microbes enhance the recruitment potential of Solidago and its ability to outcompete native plants, thereby providing strong evidence for the enhanced mutualism hypothesis. On the other hand, to some extent this outperformance of Solidago in the presence of soil microbes seems to be unbeneficial to control its rapid expansion, particularly in some ranges where this enhanced mutualism dominates over other mechanisms.

  8. In-vitro effect of flavonoids from Solidago canadensis extract on glutathione S-transferase.

    Science.gov (United States)

    Apáti, Pál; Houghton, Peter J; Kite, Geoffrey; Steventon, Glyn B; Kéry, Agnes

    2006-02-01

    Solidago canadensis is typical of a flavonoid-rich herb and the effect of an aqueous ethanol extract on glutathione-S-transferase (GST) activity using HepG2 cells was compared with those of the flavonol quercetin and its glycosides quercitrin and rutin, found as major constituents. The composition of the extract was determined by HPLC and rutin was found to be the major flavonoidal component of the extract. Total GST activity was assessed using 1-chloro-2,4-dinitrobenzene as a substrate. The glycosides rutin and quercitrin gave dose-dependent increases in GST activity, with a 50% and 24.5% increase at 250 mM, respectively, while the aglycone quercetin inhibited the enzyme by 30% at 250 mM. The total extract of the herb gave an overall dose-dependent increase, the fractions corresponding to the flavonoids showed activating effects while those containing caffeic acid derivatives were inhibitory. The activity observed corresponds to that reported for similar compounds in-vivo using rats, thus the HepG2 cell line could serve as a more satisfactory method of assessing the effects of extracts and compounds on GST.

  9. Observation of sandhill cranes' (Grus canadensis) flight behavior in heavy fog

    Science.gov (United States)

    Kirsch, Eileen M.; Wellik, Mike J.; Suarez, Manuel J.; Diehl, Robert H.; Lutes, Jim; Woyczik, Wendy; Krapfl, Jon; Sojda, Richard S.

    2015-01-01

    The behaviors of birds flying in low visibility conditions remain poorly understood. We had the opportunity to monitor Sandhill Cranes (Grus canadensis) flying in heavy fog with very low visibility during a comprehensive landscape use study of refuging cranes in the Horicon Marsh in southeastern Wisconsin. As part of the study, we recorded flight patterns of cranes with a portable marine radar at various locations and times of day, and visually counted cranes as they departed the roost in the morning. We compared flight patterns during a fog event with those recorded during clear conditions. In good visibility, cranes usually departed the night roost shortly after sunrise and flew in relatively straight paths toward foraging areas. In fog, cranes departed the roost later in the day, did not venture far from the roost, engaged in significantly more circling flight, and returned to the roost site rather than proceeding to foraging areas. We also noted that compared to mornings with good visibility, cranes flying in fog called more frequently than usual. The only time in this 2-year study that observers heard young of the year calling was during the fog event. The observed behavior of cranes circling and lingering in an area while flying in poor visibility conditions suggests that such situations may increase chances of colliding with natural or anthropogenic obstacles in the vicinity.

  10. Biochemometrics to Identify Synergists and Additives from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal).

    Science.gov (United States)

    Britton, Emily R; Kellogg, Joshua J; Kvalheim, Olav M; Cech, Nadja B

    2018-03-23

    A critical challenge in the study of botanical natural products is the difficulty of identifying multiple compounds that may contribute additively, synergistically, or antagonistically to biological activity. Herein, it is demonstrated how combining untargeted metabolomics with synergy-directed fractionation can be effective toward accomplishing this goal. To demonstrate this approach, an extract of the botanical goldenseal ( Hydrastis canadensis) was fractionated and tested for its ability to enhance the antimicrobial activity of the alkaloid berberine (4) against the pathogenic bacterium Staphylococcus aureus. Bioassay data were combined with untargeted mass spectrometry-based metabolomics data sets (biochemometrics) to produce selectivity ratio (SR) plots, which visually show which extract components are most strongly associated with the biological effect. Using this approach, the new flavonoid 3,3'-dihydroxy-5,7,4'-trimethoxy-6,8- C-dimethylflavone (29) was identified, as were several flavonoids known to be active. When tested in combination with 4, 29 lowered the IC 50 of 4 from 132.2 ± 1.1 μM to 91.5 ± 1.1 μM. In isolation, 29 did not demonstrate antimicrobial activity. The current study highlights the importance of fractionation when utilizing metabolomics for identifying bioactive components from botanical extracts and demonstrates the power of SR plots to help merge and interpret complex biological and chemical data sets.

  11. Metabolomic Profiling of the Nectars of Aquilegia pubescens and A. Canadensis.

    Directory of Open Access Journals (Sweden)

    Christos Noutsos

    Full Text Available To date, variation in nectar chemistry of flowering plants has not been studied in detail. Such variation exerts considerable influence on pollinator-plant interactions, as well as on flower traits that play important roles in the selection of a plant for visitation by specific pollinators. Over the past 60 years the Aquilegia genus has been used as a key model for speciation studies. In this study, we defined the metabolomic profiles of flower samples of two Aquilegia species, A. Canadensis and A. pubescens. We identified a total of 75 metabolites that were classified into six main categories: organic acids, fatty acids, amino acids, esters, sugars, and unknowns. The mean abundances of 25 of these metabolites were significantly different between the two species, providing insights into interspecies variation in floral chemistry. Using the PlantSEED biochemistry database, we found that the majority of these metabolites are involved in biosynthetic pathways. Finally, we explored the annotated genome of A. coerulea, using the PlantSEED pipeline and reconstructed the metabolic network of Aquilegia. This network, which contains the metabolic pathways involved in generating the observed chemical variation, is now publicly available from the DOE Systems Biology Knowledge Base (KBase; http://kbase.us.

  12. How Respiratory Pathogens Contribute to Lamb Mortality in a Poorly Performing Bighorn Sheep ( Ovis canadensis ) Herd.

    Science.gov (United States)

    Wood, Mary E; Fox, Karen A; Jennings-Gaines, Jessica; Killion, Halcyon J; Amundson, Sierra; Miller, Michael W; Edwards, William H

    2017-01-01

    We evaluated bighorn sheep ( Ovis canadensis ) ewes and their lambs in captivity to examine the sources and roles of respiratory pathogens causing lamb mortality in a poorly performing herd. After seven consecutive years of observed December recruitments of sheep from the remnant Gribbles Park herd in Colorado, US were captured and transported to the Thorne-Williams Wildlife Research Center in Wyoming in March 2013. Ewes were sampled repeatedly over 16 mo. In April 2014, ewes were separated into individual pens prior to lambing. Upon death, lambs were necropsied and tested for respiratory pathogens. Six lambs developed clinical respiratory disease and one lamb was abandoned. Pathology from an additional six lambs born in 2013 was also evaluated. Mycoplasma ovipneumoniae , leukotoxigenic Mannheimia spp., leukotoxigenic Bibersteinia trehalosi , and Pasteurella multocida all contributed to lamb pneumonia. Histopathology suggested a continuum of disease, with lesions typical of pasteurellosis predominating in younger lambs and lesions typical of mycoplasmosis predominating in older lambs. Mixed pathology was observed in lambs dying between these timeframes. We suspected that all the ewes in our study were persistently infected and chronically shedding the bacteria that contributed to summer lamb mortality.

  13. Echinococcus canadensis (G7) and Echinococcus granulosus sensu stricto (G1) in swine of southern Brazil.

    Science.gov (United States)

    Monteiro, D U; Botton, S A; Tonin, A A; Azevedo, M I; Graichen, D A S; Noal, C B; de la Rue, M L

    2014-05-28

    The cystic echinococcosis (CE) is an important zoonotic disease caused by the parasite Echinococcus spp. In Brazil, this parasite is present in Rio Grande do Sul (RS) state, border with Argentina and Uruguay, causing several damages to human and animal health. This study aimed to identify Echinococcus spp. in hydatid cysts of swine and evaluate the similarity of the genotypes through the phylogenetic analysis. A total of 3,101,992 swine were slaughtered in the central/northern region of RS/Brazil, during 2008-2012. Five isolates were characterized as hydatid cyst by molecular analysis, based on the mitochondrial gene cytochrome c oxidase subunit I (cox-I). The genotypes E. granulosus sensu stricto (G1) (n=2) and E. canadensis (G7) (n=3) were identified in the hydatid cysts. The swine represents a potential intermediate host for different genotypes of Echinococcus spp., besides it can contribute to the perpetuation of the parasite's life cycle in rural areas. Copyright © 2014 Elsevier B.V. All rights reserved.

  14. Mass Spectrometry and Antibody-Based Characterization of Blood Vessels from Brachylophosaurus canadensis.

    Science.gov (United States)

    Cleland, Timothy P; Schroeter, Elena R; Zamdborg, Leonid; Zheng, Wenxia; Lee, Ji Eun; Tran, John C; Bern, Marshall; Duncan, Michael B; Lebleu, Valerie S; Ahlf, Dorothy R; Thomas, Paul M; Kalluri, Raghu; Kelleher, Neil L; Schweitzer, Mary H

    2015-12-04

    Structures similar to blood vessels in location, morphology, flexibility, and transparency have been recovered after demineralization of multiple dinosaur cortical bone fragments from multiple specimens, some of which are as old as 80 Ma. These structures were hypothesized to be either endogenous to the bone (i.e., of vascular origin) or the result of biofilm colonizing the empty osteonal network after degradation of original organic components. Here, we test the hypothesis that these structures are endogenous and thus retain proteins in common with extant archosaur blood vessels that can be detected with high-resolution mass spectrometry and confirmed by immunofluorescence. Two lines of evidence support this hypothesis. First, peptide sequencing of Brachylophosaurus canadensis blood vessel extracts is consistent with peptides comprising extant archosaurian blood vessels and is not consistent with a bacterial, cellular slime mold, or fungal origin. Second, proteins identified by mass spectrometry can be localized to the tissues using antibodies specific to these proteins, validating their identity. Data are available via ProteomeXchange with identifier PXD001738.

  15. Cadmium triggers Elodea canadensis to change the surrounding water pH and thereby Cd uptake.

    Science.gov (United States)

    Javed, M Tariq; Greger, Maria

    2011-01-01

    This study was aimed to investigate the influence of Elodea canadensis shoots on surrounding water pH in the presence of cadmium and the effect of plant-induced pH on cadmium uptake. The pH change in the surrounding nutrient solution and Cd uptake by Elodea shoots were investigated after cultivation of various plant densities (1, 3, 6 plants per 500 ml) in hydroponics at a starting pH of 4.0 and in the presence of different concentrations of cadmium (0, 0.1, 0.5 microM). Cadmium uptake was also investigated at different constant pH (4.0, 4.5, 5.5 and 6.5). To investigate if the pH change arose from photosynthetic activities, plants were grown under light, darkness or in the presence of a photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and 0.5 microM cadmium in the solution. Elodea had an ability to increase the surrounding water pH, when the initial pH was low, which resulted in increased accumulation of Cd. The higher the plant density, the more pronounced was the pH change. The pH increase was not due to the photosynthetic activity since the pH rise was more pronounced under darkness and in the presence of DCMU. The pH increase by Elodea was triggered by cadmium.

  16. Ecological life histories of the three aquatic nuisance plants, Myriophyllum spicatum, Potamogeton crispus and Elodea canadensis

    Science.gov (United States)

    Nichols, S.A.; Shaw, B.H.

    1986-01-01

    The life histories of Myriophyllum spicatum L., Elodea canadensis Michx., and Potamogeton crispus L., serious aquatic nuisances in many regions of the world, are reviewed to provide insights into the life style of successful aquatic nuisance plants. Specifically, their distribution and spread in North America; their life cycle, productive and reproductive potential; and their ecosystem relationships are reviewed. Hopefully this review will improve a manager's ability to deal with aquatic nuisance problems. It also provides suggestions for basic research needed to develop more effective management practices. It was found that all three species possess a number of adaptations, including an ability to rapidly propagate vegetatively, an opportunistic nature for obtaining nutrients, a life cycle that favors cool weather, and a number of mechanisms which enhance photosynthetic efficiency, which allow them to proliferate. These three species do provide benefits to the ecosystem through their roles in materials cycling and energy flow. Therefore, management of these species should take an integrated approach which recognizes these benefits. The life history information available about the three species varies tremendously; however, a better understanding of resource gain and allocation is needed to manage all three species. Specifically, more research is needed to provide a better understanding of: 1) the role bicarbonate plays in photosynthesis, 2) the role roots play in supplying CO2 to the plabts, 3) resource accumulation and allocation under different temperature and light regimes, 4) resource allocation on a seasonal basis, and 5) nutrient cycling under different management regimes. ?? 1986 Dr W. Junk Publishers.

  17. A Comparative Experimental Analysis of Tribological Properties Between Commercial Mineral Oil and Neat Castor Oil using Taguchi Method in Boundary Lubrication Regime

    Directory of Open Access Journals (Sweden)

    S. Bhaumik

    2016-03-01

    Full Text Available The present work aims to carry out a comparative experimental analysis of tribological properties of 372cSt mineral oil and 229cSt castor oil. The antiwear and extreme pressure properties of both the oils have been analysed according to ASTM G 99 and ASTM D-2783 standards. The surfaces of the balls and pins after tribo tests have been analysed using Scanning Electron Microscope (SEM images. The present test results predict that the antiwear and extreme pressure properties in case of the selected commercially available mineral oil are better than neat castor oil. Both coefficient of friction and frictional force increased by 16-42 % and 16-35 % respectively in case of neat castor oil as compared with that of the mineral oil. The load carrying capacity in case of mineral oil has been found to be 250 kg while in case of castor oil it has been found to be 126 kg. SEM images show a higher plastic deformation in case of castor oil as compared to the mineral oil. Both mineral oil and castor oil showed surface deteriorations and increase in roughness after 40 hrs of intermittent running during scuffing test. Thus, the present investigation indicates that the tribological properties of neat castor are inferior to that of mineral oil although close observation of the results shows that both AW and EP properties of neat castor oil are much closer to that of commercial mineral oil. Thus, the present work would help in formulating castor oil based new bio lubricants with better anti wear and extreme pressure properties

  18. Evaluation of the cadmium and lead phytoextraction by castor bean (Ricinus communis L.) in hydroponics

    Science.gov (United States)

    Niu, Z. X.; Sun, L. N.

    2017-06-01

    Phytoextraction has been considered as an innovative method to remove toxic metals from soil; higher biomass plants such as castor bean (Ricinus communis L.) have already been considered as a hyperaccumulating candidate. In the present study, castor bean was used to accumulate the cadmium and lead in hydroponic culture, and the root exudates and biomass changes were analyzed. Results demonstrated that ratios of aerial biomass/ root biomass (AW/RW) in treatments declined with concentrations of Cd or Pb. Optical density (OD) at 190 nm and 280 nm of root exudates observed in Cd and Pb treatments were lower than the control. In single Cd or Pb treatments, bioconcentration factors (BCF) of Cd or Pb increased with time and decreased with concentrations, the highest BCFs appeared in Cd5 (14.36) and Pb50 (6.48), respectively. Cd-BCF or Pb-BCF showed positive correlations with AW/RW ratios and OD values, and they were negative correlated with Cd and Pb concentration. Results in this study may supply useful information for phytoremediation of soil contaminated with cadmium and lead in situ.

  19. Quivers For Special Fuel Rods-Disposal Of Special Fuel Rods In CASTOR V Casks

    International Nuclear Information System (INIS)

    Bannani, Amin; Cebula, Wojciech; Buchmuller, Olga; Huggenberg, Roland; Helmut Kuhl

    2015-01-01

    While GNS casks of the CASTOR family are a suitable means to transfer fuel assemblies (FA) from the NPP to an interim dry storage site, Germanys phase-out of nuclear energy has triggered the demand for an additional solution to dispose of special fuel rods (SFR), normally remaining in the fuel pond until the final shutdown of the NPP. SFR are fuel rods that had to be removed from fuel assemblies mainly due to their special condition, e. g. damages in the cladding of the fuel rods which may have occurred during reactor operations. SFR are usually stored in the spent fuel pond after they are removed from the FA. The quiver for special fuel rods features a robust yet simple design, with a high mechanical stability, a reliable leak-tightness and large safety margins for future requirements on safety analysis. The quiver for special fuel rods can be easily adapted to a large variety of different damaged fuel rods and tailored to the specific need of the customer. The quiver for special fuel rods is adaptable e.g. in length and diameter for use in other types of transport and storage casks and is applicable in other countries as well. The overall concept presented here is a first of its kind solution for the disposal of SFRs via Castor V-casks. This provides an important precondition in achieving the status 'free from nuclear fuel' of the shut down German NPPs

  20. Integral process of obtaining glycerol as a by-product of biodiesel production from castor oil

    Directory of Open Access Journals (Sweden)

    Leonel Romero

    2012-12-01

    Full Text Available The biodiesel is obtained from about 10 years ago in Europe, and now that it has taken hold as fuel for diesel engines, it is expected a clear increase in the production of this class of fuels in a the near future. The biodiesel is derived from the transesterification reaction of castor oil with methanol, which is the main by-product the glycerol with an approximate content of 10%. Besides catalyst residuals, soaps, methanol traces, mono and diglycerides in small percentages are presented. This study proposes the separation, purification and characterization of the glycerol obtained from the transesterificación reaction of the castor oil, in order to be able to market it in the national or international market, so that it fulfills the standards of quality, which means getting a pure glycerol and the appropriate physico-chemical characteristics and techniques. The glycerin-methyl esters separation is carried out by decantation being obtained a percentage of around 70% glycerol. This percentage is subsequently increased through the purification process, using hydrochloric acid. Glycerol characterization was carried out by physicochemical and organoleptic tests. The purification process allowed us to obtain a glycerol with a percentage of purity close to 98%. It was also tested by comparison with theoretical data that remnants influenced in the physiochemical properties

  1. Energetic characterization of the husk of the castor bean fruits of the variety Guarani

    Energy Technology Data Exchange (ETDEWEB)

    Teramoto, Erico Tadao; Sousa, Frederico Faula; Castro Neto, Pedro [Universidade Federal de Lavras (DEG/UFLA), MG (Brazil). Dept. de Engenharia; Trugilho, Paulo Fernando [Universidade Federal de Lavras (DCF/UFLA), MG (Brazil). Dept. de Ciencias Florestais; Fraga, Antonio Carlos [Universidade Federal de Lavras (DAG/UFLA), MG (Brazil). Dept. de Agricultura; Nunes, Ramon Rachide [Universidade Federal de Lavras (DAG/UFLA), MG (Brazil). Dept. de Quimica

    2008-07-01

    The knowledge of the physical properties and of the calorific power and of the immediate chemical analysis certain products are interesting for the development of new technologies and equipment for your processing or use as fuel. This way, due to the scarce readiness of referring works for the husk of the castor bean fruits, the present study seeks complemental the available data in the literature. For such were certain the following properties of the husk of the castor bean fruits of variety Guarani: angle of repose, hectolitre weigh, moisture, ash content, volatile material content, fixed carbon content and superior calorific power and inferior calorific power. The following values were found: 42 deg for the angle of repose, 13.92 kg for the hectolitre weight, 4223.19 kcal/kg for the superior calorific power, 3829.92 kcal/kg for the inferior calorific power, 8.21% for the moisture, 9.86% for the ash content, 73.67% for the volatile material content and 8.26% for the fixed carbon content. (author)

  2. Production of Particleboards from Hevea brasiliensis Clones and Castor Oil-based Polyurethane Resin

    Directory of Open Access Journals (Sweden)

    Maristela Gava

    2015-08-01

    Full Text Available The economic exploitation of rubber tree (Hevea brasiliensis usage is primarily directed toward latex extraction. After the productive life of the rubber tree forest, the managed area is harvested for planting reformulation. The harvested wood is most often used for energy generation purposes. The aim of this work was to study the feasibility of using rubberwood waste and castor oil-based polyurethane resin in the production of particleboards. Homogeneous and heterogeneous panels were made with nominal dimensions of 500 x 500 mm and thicknesses of 10 mm using particles from GT1 and RRIM600 clones of the rubber tree and 12% castor oil-based polyurethane adhesive. The panels were pressed at 140 °C for 12 min with 40 kgf/cm2 specific pressure. Density, moisture content, thickness swelling, water absorption for 2 and 24 h, static bending, and internal bonding determinations were performed according to the Brazilian Standard (NBR 14810-3 (2006 for the physical-mechanical panel characterization. The results show that using Hevea brasiliensis in particleboard production is viable. However, multilayer boards exhibited better results.

  3. Performance of maleated castor oil based plasticizer on rubber: rheology and curing characteristic studies

    Science.gov (United States)

    Indrajati, I. N.; Dewi, I. R.

    2017-07-01

    The objective of this study was to evaluate the performance of maleated castor oil (MACO) as plasticizer on natural rubber (NR), ethylene propylene diene monomer (EPDM), and nitrile butadiene rubber (NBR). The parameter studied were involving rheological, curing and swelling properties. The MACOs were prepared by an esterification reaction between castor oil (CO) and maleic anhydride (MAH) with the help of xylene as water entrainer to improve water removal. Resulting oils then applied as a plasticizer in each of those rubbers within a fixed loading of 5 phr. Comparison has been made to evaluate the performance of MACO and conventional plasticizer (paraffinic oil for NR and EPDM, DOP for NBR) on each rubber. Rheology, curing characteristic and swelling of each rubber were studied. The results showed that rubber (NR/EPDM/NBR) plasticized with MACO had given similar flow characteristic to conventional plasticizers. MACO exhibited slow curing, confirmed by higher t90, but the scorch safety was of the same magnitude. MAH loading tended to decrease the flow properties and curing rate, while scorch time (ts2) was independent.

  4. CASTOR-V/21 PWR spent fuel storage cask performance test

    International Nuclear Information System (INIS)

    Creer, J.M.; Schoonen, D.H.

    1986-01-01

    Performance testing of a CASTOR-V/21 PWR spent fuel storage cask manufactured by Gesellschaft fur Nuklear Service (GNS) was performed as part of a cooperative program between Virginia Power and the US Department of Energy. The performance test consisted of obtaining cask handling experience and heat transfer, shielding, and limited fuel integrity data. Five heat transfer test runs were performed with 21 Surry reactor spent fuel assemblies generating approximately 28 kW. Test runs were performed with vacuum, nitrogen, and helium backfills in both vertical and horizontal orientations. Cask exterior surface gamma and neutron dose rates were measured with the cask fully loaded. Gas samples were obtained at the beginning and end of each run with nitrogen or helium backfills to verify fuel integrity. The heat transfer performance of the CASTOR-V/21 cask was exceptionally good. Peak clad temperatures with helium and nitrogen backfills in a vertical orientation and with helium in a horizontal orientation were less than 380 0 C. Vertical vacuum and horizontal nitrogen runs resulted in peak clad temperatures over 380 0 , but the temperatures were not excessively high ( 0 C). The shielding performance of the cask met the design expectation of less than 200 mrem/h. Cask surface dose rates of <75 mrem/h can easily be established with minor gamma shielding design refinements if desired. Gas samples obtained during testing indicated no leaking fuel rods were present in the cask. It was concluded that the cask performed satisfactorily from heat transfer and shielding perspectives

  5. Castor-1C spent fuel storage cask decay heat, heat transfer, and shielding analyses

    International Nuclear Information System (INIS)

    Rector, D.R.; McCann, R.A.; Jenquin, U.P.; Heeb, C.M.; Creer, J.M.; Wheeler, C.L.

    1986-12-01

    This report documents the decay heat, heat transfer, and shielding analyses of the Gesellschaft fuer Nuklear Services (GNS) CASTOR-1C cask used in a spent fuel storage demonstration performed at Preussen Elektra's Wurgassen nuclear power plant. The demonstration was performed between March 1982 and January 1984, and resulted in cask and fuel temperature data and cask exterior surface gamma-ray and neutron radiation dose rate measurements. The purpose of the analyses reported here was to evaluate decay heat, heat transfer, and shielding computer codes. The analyses consisted of (1) performing pre-look predictions (predictions performed before the analysts were provided the test data), (2) comparing ORIGEN2 (decay heat), COBRA-SFS and HYDRA (heat transfer), and QAD and DOT (shielding) results to data, and (3) performing post-test analyses if appropriate. Even though two heat transfer codes were used to predict CASTOR-1C cask test data, no attempt was made to compare the two codes. The codes are being evaluated with other test data (single-assembly data and other cask data), and to compare the codes based on one set of data may be premature and lead to erroneous conclusions

  6. Forensic determination of ricin and the alkaloid marker ricinine from castor bean extracts.

    Science.gov (United States)

    Darby, S M; Miller, M L; Allen, R O

    2001-09-01

    Liquid chromatography/mass spectrometry (LC/ MS) and matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS methods were developed for the presumptive identification of ricin toxin and the alkaloid marker ricinine from crude plant materials. Ricin is an extremely potent poison, which is of forensic interest due to its appearance in terrorism literature and its potential for use as a homicide agent. Difficulties arise in attempting to analyze ricin because it is a large heterogeneous protein with glycosylation. The general protein identification scheme developed uses LC/MS or MALDI-TOF for size classification followed by the use of the same instrumentation for the analysis of the tryptic digest. Fragments of the digest can be searched in an online database for tentative identification of the unknown protein and then followed by comparison to authentic reference materials. LC fractionation or molecular weight cutoff filtration was used for preparation of the intact toxin before analysis. Extracts from two types of castor beans were prepared using a terrorist handbook procedure and determined to contain 1% ricin. Additionally, a forensic sample suspected to contain ricin was analyzed using the presented identification scheme (data not shown). The identification of the alkaloid ricinine by GC/MS and LC/MS was shown to be a complementary technique for the determination of castor bean extracts.

  7. Synthesis of biodiesel from castor oil: Silent versus sonicated methylation and energy studies

    International Nuclear Information System (INIS)

    Sáez-Bastante, J.; Pinzi, S.; Jiménez-Romero, F.J.; Luque de Castro, M.D.; Priego-Capote, F.; Dorado, M.P.

    2015-01-01

    Highlights: • Sonicated transesterification leads to higher conversion than conventional one. • Energy consumption required by conventional and ultrasound-assisted transesterification was compared. • Ultrasound-assisted methylation is more competitive in terms of energy than conventional one. - Abstract: In recent years, biodiesel is evolving to be one of the most employed biofuels for partial replacement of petrodiesel. The most widely used feedstocks for biodiesel production are vegetable oils. Among them, castor oil presents two interesting features as biodiesel raw material; on one hand, it does not compete with edible oils; on the other, the cultivar does not require high inputs. In this research, a comparison between conventional and ultrasound-assisted transesterification was carried out in terms of castor oil methyl ester (COME) yield and energy efficiency. Results show that sonicated transesterification leads to higher COME yields under lower methanol-to-oil molar ratio, lower amount of catalyst, shorter reaction time and lower amount of energy required. Ultrasound-assisted transesterification parameters were optimized resulting in the following optimum conditions: 20 kHz fixed frequency, 70% duty cycle, 40% sonication amplitude, 4.87 methanol-to-oil molar ratio, 1.4% w/w amount of catalyst and 3 sonication cycles (3 min 48 s) that provided 86.57% w/w COME yield. The energy required along each type of transesterification was measured leading to the conclusion that sonicated transesterification consumes a significant lower amount of energy than conventional one, thus achieving higher COME yield

  8. Bio-based polyurethane prepared from Kraft lignin and modified castor oil

    Directory of Open Access Journals (Sweden)

    L. B. Tavares

    2016-11-01

    Full Text Available Current challenges highlight the need for polymer research using renewable natural sources as a substitute for petroleum-based polymers. The use of polyols obtained from renewable sources combined with the reuse of industrial residues such as lignin is an important agent in this process. Different compositions of polyurethane-type materials were prepared by combining technical Kraft lignin (TKL with castor oil (CO or modified castor oil (MCO1 and MCO2 to increase their reactivity towards diphenylmethane diisocyanate (MDI. The results indicate that lignin increases the glass transition temperature, the crosslinking density and improves the ultimate stress especially for those prepared from MCO2 and 30% lignin content from 8.2 MPa (lignin free to 23.5 MPa. Scanning electron microscopy (SEM micrographs of rupture surface after uniaxial tensile tests show ductile-to-brittle transition. The results show the possibility to develop polyurethane-type materials, varying technical grade Kraft lignin content, which cover a wide range of mechanical properties (from large elastic/low Young modulus to brittle/high Young modulus polyurethanes.

  9. Bioplastic from Chitosan and Yellow Pumpkin Starch with Castor Oil as Plasticizer

    Science.gov (United States)

    Hasan, M.; Rahmayani, R. F. I.; Munandar

    2018-03-01

    This study has been conducted on bioplastic synthesis of chitosan and yellow pumpkin starch (Cucurbita moschata) with castor oil as plasticizer. The purpose of this study is to determine the characteristics of the effect of chitosan and starch composition of pumpkins against solvent absorption, tensile strength and biodegradable. The first stage of the research is the making of bioplastic by blending yellow pumpkin starch, chitosan and castor oil. Further, it tested the absorption capacity of the solvent, tensile strength test, and biodegradable analysis. The optimum absorption capacity of the solvent is obtained on the composition of Pumpkin/Chitosan was 50/50 in H2O and C2H5OH solvent. Meanwhile the optimum absorbency in HCl and NaOH solvents is obtained by 60/40 composition. The characterization of the optimum tensile strength test was obtained on the 40/60 composition of 6.787 ± 0.274 Mpa and the fastest biodegradation test process within 5-10 days occurred in the 50/50 composition. The more chitosan content the higher the value of tensile strength test obtained, while the fastest biodegradation rate occureds in the composition of yellow pumpkin starch and chitosan balanced 50:50.

  10. SOIL EXCHANGEABLE ALUMINUM INFLUENCING THE GROWTH AND LEAF TISSUE MACRONUTRIENTS CONTENT OF CASTOR PLANTS

    Directory of Open Access Journals (Sweden)

    ROSIANE DE LOURDES SILVA DE LIMA

    2014-01-01

    Full Text Available Three castor ( Ricinus communis genotypes were studied regarding tolerance to high exchange factorial distribution of five doses of exchangeable aluminum added to the soil (0, 0.15, 0.30, 0.60, and 1.20 cmol c dm - 3 and three castor genotypes (BRS Nordestina, BRS Paraguaçu, and Lyra. The plants were raised in pots in a greenhouse. At 53 days after emergence, data were taken on plant height, leaf area, dry mass of shoot and root, and leaf tissue content of macronutrients. The most sensitive genotype was the cv. BRS Nordestina, in which the shoot and root dry weight in the highest aluminum content were reduced to 12.9% and 16.2% of the control treatment, respectively. The most tolerant genotype was the hybrid Lyra, in which the shoot and root dry weight in the maximum content of aluminum were reduced to 43.5% and 42.7% of the control treatment, respectively.The increased exchangeable aluminum affected the leaf nutrient content, and the intensity of the response was different among cultivars. The aluminum toxicity increased N, Ca, and Mg contents and reduced on P, K, and S contents. The cv. BRS Nordestina had a drastic shoot dry weight reduction associated with an intense increment in the N leaf content. Thus, the N increment was caused by a concentration effect caused by the limited growth.

  11. Effect of the organic matter and soil water deficit on the castor bean inflorescences emission

    Energy Technology Data Exchange (ETDEWEB)

    Lacerda, Rogerio Dantas de; Araujo, Ester Luiz de; Nascimento, Elka Costa Santos; Barros Junior, Genival [Universidade Federal de Campina Grande (UFCG), PB (Brazil); Guerra, Hugo O. Carvallo; Chaves, Lucia Helena G. [Universidade Federal de Campina Grande (UAEAg/UFCG), PB (Brazil). Unidade Academica de Engenharia Agricola

    2008-07-01

    The castor bean culture has become important due to the several applications of its oil, which constitutes one of the best row materials for biodiesel manufacturing, and the base for several other industrial products. The objective of the present work was to study the effect of different soil water and soil organic matter on the castor bean inflorescence emission. The experiment was conducted from April to August 2006 under Greenhouse conditions using a randomized block 2x4 factorial design with two soil organic mater content (5.0 g.kg{sup -1} e 25.0 g.kg{sup -1}), four levels of available water (100, 90, 80 e 70% ) and three replicates. For this, 24 plastic containers, 75 kg capacity, were used on which was grown one plant 120 days after the seedling. When flowering occurred it was measured the number, the time required for the emission and the height of the emissions. The results were analyzed statistically; for the qualitative factor (with and without organic matter) the treatment means were compared through the Tukey test. For the quantitative ones (water levels) regressions were used. The time for the emission of the inflorescences was affected significantly by the organic matter and the available soil water content for plants. The number of inflorescences was affected positively by both treatments. (author)

  12. Influence of the organic matter and soil water deficit on the castor bean absolute growth rate

    Energy Technology Data Exchange (ETDEWEB)

    Lacerda, Rogerio Dantas de; Guerra, Hugo O. Carvallo; Chaves, Lucia Helena G. [Universidade Federal de Campina Grande (UAEAg/UFCG), PB (Brazil). Unidade Academica de Engenharia Agricola; Araujo, Ester Luiz de; Nascimento, Elka Costa Santos; Barros Junior, Genival [Universidade Federal de Campina Grande (UFCG), PB (Brazil)

    2008-07-01

    Even when under low precipitations conditions, the castor bean production decrease, it constitutes a very good alternative. It has an elevated economical importance, because from the plant it is used their leaves, stem and seeds. From the stem it is obtained cellulose for the paper industry, from the leaves textile products and from the seeds oil and tort. The oil is the only glycerin soluble in alcohol and the base for several industrial products such as the biodiesel. The objective of the present work was to study the effect of different soil water and soil organic matter on the castor bean, BRS 188 cultivar rate growth. The experiment was conducted from April to August 2006 under greenhouse conditions using a randomized block 2x4 factorial design with two soil organic mater content (5.0 g.kg{sup -1} e 25.0 g.kg{sup -1}), four levels of available water (100, 90, 80 e 70% ) and three replicates. For this, 24 plastic containers, 75 kg capacity, were used on which was grown one plant 120 days after the seedling. At regular intervals the plant height was measured and the results analyzed statistically. For the qualitative treatments (with and without organic matter) the treatment means were compared through the Tukey test. For the quantitative ones (water levels) regressions were used. It was observed that both, organic matter and available water for plants proportionated benefit effects to the growth rate of the plant. (author)

  13. The catalytic microwave synthesis of biodegradable polyester polyols based on castor oil and l-lactide

    Science.gov (United States)

    Kojić, D.; Erceg, T.; Vukić, N.; Teofilović, V.; Ristić, I.; Budinski-Simendić, J.; Aleksić, V.

    2017-01-01

    Various strategies for achieving a functional poly(lactic acid) (PLA) have been developed such as ring-opening copolymerization with a functional monomer, the use of functional initiator and various post polymerization modifications. It is possible to obtain the star shaped polymer using natural oil with at least three OH groups as an initiator. It was estimated that despite of low-molecular mass of star-shaped PLA, the hydrophobic castor oil central core influenced the slow degradation rate in the case of injectable biomedical application. The star-shaped polymers with low-molecular-mass have a lower melt viscosity correlated with linear counterparts. In soft tissue reparation the polymer viscosity increases with fluid body contact and the solid implant can be formed. To ensure liquid state at injection temperature the low molar mass polymer is favorable. There is a particular size for each macromolecular chains at which chain entanglement occurs. In this work the influence of the l-lactide (LA) and the castor oil (CO) contents on the size of biodegradable branched polyester polyols was studied. The average molecular masses of synthesized polymers were estimated by GPC procedure. In sample formulations the [LA]/[CO] ratios were from to 113 to 533. Mn values for obtained polymers were from 5000 to 20000 Da. The molecular mass distribution for the resulting polymers was between 1.09 and 1.37.

  14. Phosphorylation of glyoxysomal malate synthase from castor oil seed endosperm and cucumber cotyledon

    International Nuclear Information System (INIS)

    Yang, Y.P; Randall, D.D.

    1989-01-01

    Glyoxysomal malate synthase (MS) was purified to apparent homogeneity from 3-d germinating castor oil seed endosperm by a relatively simple procedure including two sucrose density gradient centrifugations. Antibodies raised to the caster oil seed MS crossreacted with MS from cucumber cotyledon. MS was phosphorylated in both tissues in an MgATP dependent reaction. The phosphorylation pattern was similar for both enzymes and both enzymes were inhibited by NaF, NaMo, (NH 4 )SO 4 , glyoxylate and high concentration of MgCl 2 (60 mM), but was not inhibited by NaCl and malate. Further characterization of the phosphorylation of MS from castor oil seed endosperms showed that the 5S form of MS is the form which is labelled by 32 P. The addition of exogenous alkaline phosphatase to MS not only decreased enzyme activity, but could also dephosphorylate phospho-MS. The relationship between dephosphorylation of MS and the decrease of MS activity is currently under investigation

  15. Green superlubricity of Nitinol 60 alloy against steel in presence of castor oil

    Science.gov (United States)

    Zeng, Qunfeng; Dong, Guangneng; Martin, Jean Michel

    2016-07-01

    In the present work, first, we show that sliding Nitinol 60 alloy against steel under castor oil lubrication exhibits a new case of superlubricity (coefficient of friction CoF ≪ 0.01). So far, CoF below 0.01 have never been achieved under boundary lubrication at high contact pressure and in presence of vegetable oil as a green lubricant. Next, it is demonstrated that superlubricity is controlled by tribochemical reactions, involving chemical degradation of castor oil and the formation of metal oxy-hydroxides. Finally, to explain these findings, we propose a novel superlubricity mechanism consisting of hexanoic acid molecules intercalated between nickel and iron oxy-hydroxide lamellar layers, a structure very similar to the one found in Fe-Ni batteries. We propose that superlubricity is achieved due to repulsive electrostatic forces acting between the intercalated metal oxy-hydroxide lamellar compounds. This system would be suitable for practical engineering applications in many fields including biotechnologies.

  16. The use of castor oil and ricinoleic acid in lead chalcogenide nanocrystal synthesis

    Science.gov (United States)

    Kyobe, Joseph W. M.; Mubofu, Egid B.; Makame, Yahya M. M.; Mlowe, Sixberth; Revaprasadu, Neerish

    2016-08-01

    A green solution-based thermolysis method for the synthesis of lead chalcogenide (PbE, E = S, Se, Te) nanocrystals in castor oil (CSTO) and its isolate ricinoleic acid (RA) is described. The blue shift observed from the optical spectra of CSTO and RA-capped PbE nanocrystals (NCs) confirmed the evidence of quantum confinement. The dimensions of PbE NCs obtained from NIR absorption spectra, transmission electron microscopy (TEM), and X-ray diffraction (XRD) studies were in good agreement. The particle sizes estimated were in the range of 20, 25, and 130 nm for castor oil-capped PbS, PbSe, and PbTe, respectively. Well-defined close to cubic-shaped particles were observed in the scanning electron microscopy (SEM) images of PbSe and PbTe nanocrystals. The high-resolution TEM and selective area electron diffraction (SAED) micrographs of the as-synthesized crystalline PbE NCs showed distinct lattice fringes with d-spacing distances corroborating with the standard values reported in literature.

  17. Hypersensitivity reaction studies of a polyethoxylated castor oil-free, liposome-based alternative paclitaxel formulation.

    Science.gov (United States)

    Wang, Hongbo; Cheng, Guang; Du, Yuan; Ye, Liang; Chen, Wenzhong; Zhang, Leiming; Wang, Tian; Tian, Jingwei; Fu, Fenghua

    2013-03-01

    The commercial drug paclitaxel (Taxol) may introduce hypersensitivity reactions associated with the polyethoxylated castor oil-ethanol solvent. To overcome these problems, we developed a polyethoxylated castor oil-free, liposome-based alternative paclitaxel formulation, known as Lipusu. In this study, we performed in vitro and in vivo experiments to compare the safety profiles of Lipusu and Taxol, with special regard to hypersensitivity reactions. First, Swiss mice were used to determine the lethal dosages, and then to evaluate hypersensitivity reactions, followed by histopathological examination and enzyme-linked immunosorbent assays (ELISAs) of serum SC5b-9 and lung histamine. Additionally, healthy human serum was used to analyze in vitro complement activation. Finally, an MTT assay was used to determine the in vitro anti-proliferation activity. Our data clearly showed that Lipusu displayed a much higher safety margin and did not induce hypersensitivity or hypersensitivity-related lung lesions, which may be associated with the fact that Lipusu did not activate complement or increase histamine release in vivo. Moreover, Lipusu did not promote complement activation in healthy human serum in vitro, and demonstrated anti-proliferative activity against human cancer cells, similar to that of Taxol. Therefore, the improved formulation of paclitaxel, which exhibited a much better safety profile and comparable cytotoxic activity to Taxol, may bring a number of benefits to cancer patients.

  18. Isocyanate-functionalized chitin and chitosan as gelling agents of castor oil.

    Science.gov (United States)

    Gallego, Rocío; Arteaga, Jesús F; Valencia, Concepción; Franco, José M

    2013-06-03

    The main objective of this work was the incorporation of reactive isocyanate groups into chitin and chitosan in order to effectively use the products as reactive thickening agents in castor oil. The resulting gel-like dispersions could be potentially used as biodegradable lubricating greases. Three different NCO-functionalized polymers were obtained: two of them by promoting the reaction of chitosan with 1,6-hexamethylene diisocyanate (HMDI), and the other by using chitin instead of chitosan. These polymers were characterized through 1H-NMR, FTIR and thermogravimetric analysis (TGA). Thermal and rheological behaviours of the oleogels prepared by dispersing these polymers in castor oil were studied by means of TGA and small-amplitude oscillatory shear (SAOS) measurements. The evolution and values of the linear viscoelasticity functions with frequency for -NCO-functionalized chitosan- and chitin-based oleogels are quite similar to those found for standard lubricating greases. In relation to long-term stability of these oleogels, no phase separation was observed and the values of viscoelastic functions increase significantly during the first seven days of ageing, and then remain almost constant. TGA analysis showed that the degradation temperature of the resulting oleogels is higher than that found for traditional lubricating greases.

  19. Castor oil as secondary carbon source for production of sophorolipids using Starmerella bombicola NRRL Y-17069.

    Science.gov (United States)

    Bajaj, Vinit Kamalkishor; Annapure, Uday S

    2015-01-01

    Sophorolipids (SLs), a prominent member of the biosurfactants family are produced in acidic and/or lactonic form by yeast Starmerella bombicola NRRL Y-17069 when grown on hydrophilic or hydrophobic or both carbon sources. In current study, ricinoleic acid rich castor oil (10%) was used as hydrophobic and glycerol (10%) was used as hydrophilic carbon source. The yields of 24.5 ± 0.25 g/l sophorolipids were analyzed by anthrone and HPLC method which further increased upto 40.24 ± 0.76 g/l sophorolipids using fed batch process at 5L fermenter level. The structures of sophorolipids synthesized on castor oil were elucidated by liquid chromatography-mass spectrometer (LC-MS), (13)C and (1)H NMR. The results indicated that the ricinoleic acid (RA) gets hydroxylated at ω-1 position but incorporated into sophorolipids through already available hydroxyl group at 12(th) position. It resulted in the production of a novel sophorolipids with hydroxyl fatty acid as side chain and has applications as surfactant for novel drug delivery, anti microbial agent, cosmetic ingredient and emulsifier.

  20. Polyurethane and polyurea nanoparticles based on polyoxyethylene castor oil derivative surfactant suitable for endovascular applications.

    Science.gov (United States)

    Morral-Ruíz, Genoveva; Melgar-Lesmes, Pedro; García, María Luísa; Solans, Conxita; García-Celma, María José

    2014-01-30

    The design of new, safe and effective nanotherapeutic systems is an important challenge for the researchers in the nanotechnology area. This study describes the formation of biocompatible polyurethane and polyurea nanoparticles based on polyoxyethylene castor oil derivative surfactant formed from O/W nano-emulsions by polymerization at the droplet interfaces in systems composed by aqueous solution/Kolliphor(®) ELP/medium chain triglyceride suitable for intravenous administration. Initial nano-emulsions incorporating highly hydrophilic materials were prepared by the phase inversion composition (PIC) method. After polymerization, nanoparticles with a small particle diameter (25-55 nm) and low polydispersity index were obtained. Parameters such as concentration of monomer, O/S weight ratio as well as the polymerization temperature were crucial to achieve a correct formation of these nanoparticles. Moreover, FT-IR studies showed the full conversion of the monomer to polyurethane and polyurea polymers. Likewise the involvement of the surfactant in the polymerization process through their nucleophilic groups to form the polymeric matrix was demonstrated. This could mean a first step in the development of biocompatible systems formulated with polyoxyethylene castor oil derivative surfactants. In addition, haemolysis and cell viability assays evidenced the good biocompatibility of KELP polyurethane and polyurea nanoparticles thus indicating the potential of these nanosystems as promising drug carriers. Copyright © 2013 Elsevier B.V. All rights reserved.

  1. Quivers For Special Fuel Rods-Disposal Of Special Fuel Rods In CASTOR V Casks

    Energy Technology Data Exchange (ETDEWEB)

    Bannani, Amin; Cebula, Wojciech; Buchmuller, Olga; Huggenberg, Roland [GNS, Essen (Germany); Helmut Kuhl [WTI, Julich (Germany)

    2015-05-15

    While GNS casks of the CASTOR family are a suitable means to transfer fuel assemblies (FA) from the NPP to an interim dry storage site, Germanys phase-out of nuclear energy has triggered the demand for an additional solution to dispose of special fuel rods (SFR), normally remaining in the fuel pond until the final shutdown of the NPP. SFR are fuel rods that had to be removed from fuel assemblies mainly due to their special condition, e. g. damages in the cladding of the fuel rods which may have occurred during reactor operations. SFR are usually stored in the spent fuel pond after they are removed from the FA. The quiver for special fuel rods features a robust yet simple design, with a high mechanical stability, a reliable leak-tightness and large safety margins for future requirements on safety analysis. The quiver for special fuel rods can be easily adapted to a large variety of different damaged fuel rods and tailored to the specific need of the customer. The quiver for special fuel rods is adaptable e.g. in length and diameter for use in other types of transport and storage casks and is applicable in other countries as well. The overall concept presented here is a first of its kind solution for the disposal of SFRs via Castor V-casks. This provides an important precondition in achieving the status 'free from nuclear fuel' of the shut down German NPPs.

  2. Assimilation and water relations of dryland castor at different intensities of solar radiation

    International Nuclear Information System (INIS)

    Balasubramanian, V.; Venkateswarlu, S.

    1995-01-01

    Primary racemes of dryland castor develop during later part of rainy season and secondaries and tertiaries develop during post-rainy season. The reproductive phase is therefore subjected to variation in soil moisture availability and solar radiation intensity. The objective of the study was to find out the influence of fluctuation in solar radiation intensity on photosynthetic rate, transpiration rate, transpiration efficiency, stomatal conductance and leaf water potential during early and late reproductive phase of castor. When photosynthetically active radiation was less than 1000 mu-mol m-2s-1, transpiration efficiency decreased because reduction in photosynthesis rate was more than that in transpiration rate. Transpiration efficiency also decreased, when radiation was above 1500 mu-mol m-2s-1 because of increase only in transpiration rate. Leaf water potential was higher during early than during late reproductive phase at similar radiation intensity. Transpiration rate was lower and transpiration efficiency was more during early phase when radiation was above 1500 mu-mol m-2s-1. Photosynthetically active radiation and leaf water potential were inversely related

  3. Influence of solubilizer PEG-40 hydrogenated castor oil on carbopol gels’ structural-mechanical properties

    Directory of Open Access Journals (Sweden)

    Ye. V. Gladukh

    2017-12-01

    Full Text Available Rheological properties affect all stages of the drug development – from development to production, the characteristics of the final products and stability. A lot of substances have complex rheological properties; their viscosity and elasticity can vary depending on conditions acting from the outside, such as stress, deformation, time factor and temperature. Concentration, stability and composition also significantly affect the rheological properties of drugs. One of the current trends in modern pharmacy is the development of drugs in the form of gels. The rheological properties of gels are significantly influenced by surface-active substances, stabilizers, solubilizers, stabilizing their structure. A special group of stabilizers are hydrogenated vegetable oils and their compounds with polymers, which have the ability to structure formation in interphase layers and in the volume of phases. For this purpose, PEG-40 hydrogenated castor oil is widely used. The aim of this work is to study the effect of hydrogenated castor oil, used as an emulsifier, solubilizer, viscosity modifier and solvent in the technology of semisolid dosage forms, on the structural and mechanical properties of carbopol gels. Materials and methods. 1% gel carbopol with additives PEG-40 hydrogenated castor oil in the concentration range from 1 to 5 % was investigated as experimental samples of the gel base. A 10 % propylene glycol additive was used as humectant and plasticizer. Structural and mechanical studies were carried out using a rotational viscometer «RheolabQC», Anton Paar (Austria with coaxial cylinders CCC27/SS. The graphs of the gels were automatically plotted using the computer program. Results. Analysis of the rheological parameters of the carbopol with PEG-40 hydrogenated castor oil gel base shows that the solubilizer has an active influence on the structural and mechanical properties of the base. Addition of PEG-40 GMM to the carbopol gel increases the yield

  4. Photosynthetic pigments and gas exchange in castor bean under conditions of above the optimal temperature and high CO2

    Directory of Open Access Journals (Sweden)

    Fabiola França Silva

    2015-08-01

    Full Text Available The castor bean plant, a Euphorbiaceae oil seed C3-metabolism rustic and drought-resistant plant, is cultivated in a wide range of environments due to its good adaptive capacity. However, given the current environmental changes, many biochemical and physiological impacts may affect the productivity of important crops, such as castor bean. This work aimed to evaluate the impacts of the castor bean gas exchange in response to high temperature and increased CO2concentration.Our experiment was conducted in a phytotron located at Embrapa Algodão in 2010. We adopted a completely randomized design, with four treatments in a factorial combination of two temperatures (30/20 and 37/30°C and two CO2 levels (400 and 800 mmol L-1; four replications were performed, obtained in five surveys over the growth cycle, for a total of 80 sample units. An infrared gas analyzer (IRGA - Infra Red Gas Analyzer was used for the quantification of the photosynthetic rate, stomatal conductance and transpiration. An increase in the atmospheric CO2 concentration and temperature negatively affected the physiology of the castor bean plants, decreasing the net rate of photosynthesis, transpiration and stomatal conductance.

  5. Local Perceptions about the Effects of Jatropha (Jatropha curcas and Castor (Ricinus communis Plantations on Households in Ghana and Ethiopia

    Directory of Open Access Journals (Sweden)

    Joleen A. Timko

    2014-10-01

    Full Text Available Biofuel plantations have been hyped as a means to reinvigorate Africa’s rural areas. Yet there is still apprehension about the negative environmental and social impacts of large-scale commercial biofuel production around rising food prices, land grabbing, ecological damage, and disruption of rural livelihoods. Given the extent of Jatropha curcas production in Ghana and Ethiopia and Castor bean (Ricinus communis in Ethiopia, this paper presents the results of a study that assessed the socio-economic implications of industrial Jatropha plantations on local livelihoods in Ghana, and of industrial Jatropha and Castor plantations on local livelihoods in Ethiopia. This study used primary data collected from 234 households in Ghana and 165 in Ethiopia. The cultivation of Jatropha and Castor has had several important effects on local livelihoods in the study sites, most notably decreases in household landholdings due to the arrival of industrial Jatropha or Castor plantations; and the resulting changes these plantations have caused in household socio-economic status, food security, fallow periods, and fodder availability. We consider how a lack of meaningful consultation between local people, their traditional authorities and the biofuel company managers, along with shortcomings in each country’s broader land acquisition process and poor land use information, may have contributed to these overall negative effects on local livelihoods. We conclude by suggesting several ways that emerging biofuel industries could be improved from the perspective of local people and their livelihoods.

  6. Changes in antioxidant and biochemical activities in castor oil-coated Capsicum annuum L. during postharvest storage.

    Science.gov (United States)

    Panigrahi, Jitendriya; Patel, Mansi; Patel, Niyati; Gheewala, Bhumi; Gantait, Saikat

    2018-06-01

    This study, for the first time, evaluates the efficiency of castor oil when used as an external coating on Capsicum annuum L., to increase postharvest storage-life at 4 ± 1 °C. The castor oil-coated fruits were successfully stored for 36 days, while the non-coated fruits could only sustain for 18 days. Throughout the storage period (at 9-day intervals), different antioxidants and biochemical assays (allied with storage) such as titratable acidity, ascorbic acid content, ferrous ion chelating activity, reducing power, DPPH scavenging activity, hydroxyl radical scavenging activity, total phenolic content, total sugar estimation, and enzymatic study of polyphenol oxidase and pectate lyase, were assessed. During storage, the castor oil-coated fruits showed a substantial decrease in titratable acidity, ascorbic acid content, total phenolic content, including antioxidant activities such as reducing power and DPPH activity; however, an increase in ferrous ion chelating activity, total soluble sugar content, polyphenol oxidase activity and initial pectate lyase activity was observed, in contrast to that of the non-coated fruits. The application of castor oil proved to be effective in delaying the ripening process of fruits during storage.

  7. 78 FR 20029 - Castor Oil, Polymer With Adipic Acid, Linoleic Acid, Oleic Acid and Ricinoleic Acid; Tolerance...

    Science.gov (United States)

    2013-04-03

    ..., Polymer With Adipic Acid, Linoleic Acid, Oleic Acid and Ricinoleic Acid; Tolerance Exemption AGENCY... from the requirement of a tolerance for residues of castor oil, polymer with adipic acid, linoleic acid... pesticide formulation. Advance Polymer Technology submitted a petition to EPA under the Federal Food, Drug...

  8. The effects of possible contamination on the radiocarbon dating of the Dead Sea Scrolls I : Castor oil

    NARCIS (Netherlands)

    Rasmussen, KL; van der Plicht, J; Cryer, FH; Doudna, G; Cross, FM; Strugnell, J; Rasmussen, Kaare L.; Cryer, Frederick H.; Cross, Frank M.

    2001-01-01

    Some fragments of the Dead Sea Scroll manuscripts were contaminated with castor oil in the late 1950s. We have conducted experiments in order to establish if the AAA pretreatment cleaning procedures conducted on Dead Sea Scroll manuscript samples in the last two dating series (Bonani et al. 1992;

  9. Design and development of low cost polyurethane biopolymer based on castor oil and glycerol for biomedical applications.

    Science.gov (United States)

    Tan, A C W; Polo-Cambronell, B J; Provaggi, E; Ardila-Suárez, C; Ramirez-Caballero, G E; Baldovino-Medrano, V G; Kalaskar, D M

    2018-02-01

    In the current study, we present the synthesis of novel low cost bio-polyurethane compositions with variable mechanical properties based on castor oil and glycerol for biomedical applications. A detailed investigation of the physicochemical properties of the polymer was carried out by using mechanical testing, ATR-FTIR, and X-ray photoelectron spectroscopy (XPS). Polymers were also tested in short term in-vitro cell culture with human mesenchymal stem cells to evaluate their biocompatibility for potential applications as biomaterial. FTIR analysis confirmed the synthesis of castor oil and glycerol based PU polymers. FTIR also showed that the addition of glycerol as co-polyol increases crosslinking within the polymer backbone hence enhancing the bulk mechanical properties of the polymer. XPS data showed that glycerol incorporation leads to an enrichment of oxidized organic species on the surface of the polymers. Preliminary investigation into in vitro biocompatibility showed that serum protein adsorption can be controlled by varying the glycerol content with polymer backbone. An alamar blue assay looking at the metabolic activity of the cells indicated that castor oil based PU and its variants containing glycerol are non-toxic to the cells. This study opens an avenue for using low cost bio-polyurethane based on castor oil and glycerol for biomedical applications. © 2017 The Authors Biopolymers Published by Wiley Periodicals, Inc.

  10. 17 alpha-hydroxyprogesterone caproate vehicle, castor oil, enhances the contractile effect of oxytocin in human myometrium in pregnancy.

    Science.gov (United States)

    O'Sullivan, Michael D; Hehir, Mark P; O'Brien, Yvonne M; Morrison, John J

    2010-05-01

    The possibility exists that the vehicle for 17-alpha-hydroxyprogesterone caproate, castor oil, exerts an effect on human uterine contractility. The aim of this study was to evaluate its effects on contractility of myometrial preparations that were obtained during pregnancy. Myometrial strips were suspended under isometric conditions. Contractility was induced with oxytocin. Strips were incubated in castor oil or physiologic salt solution and suspended for a further oxytocin challenge. Contractile integrals were compared between both groups. Strips that were exposed to castor oil demonstrated increased contractile activity that was elicited by oxytocin (mean contractility value, 165.53%+/-17.03%; n=8; P=.004), compared with control strips (mean contractility value, 72.57%+/-7.48%; n=8; P=.003). There was a significant increase in contractile activity of the castor oil-exposed strips, compared with those that were exposed to physiologic salt solution (n=8; Pcastor oil results in enhanced oxytocin-induced contractility. Copyright (c) 2010 Mosby, Inc. All rights reserved.

  11. Evaluation of the Optimal Reaction Conditions for the Methanolysis and Ethanolysis of Castor Oil Catalyzed by Immobilized Enzymes

    DEFF Research Database (Denmark)

    Andrade, Thalles Allan; Al-Kabalawi, Ibrahim; Errico, Massimiliano

    This study aims to compare the efficiency of the transesterification of castor oil with methanol and ethanol as part of the biodiesel production, using immobilized enzyme Lipozyme IM as catalyst. Different reaction conditions were evaluated and optimized, including the reaction temperature, alcohol...

  12. Pengaruh Konsentrasi Minyak Kelapa Murni (Virgin Coconut Oil) dan Minyak Jarak (Castor Oil) terhadap Sifat Fisikokimia dan Organoleptik Sabun Mandi Cair

    OpenAIRE

    Asri Widyasanti; Shayana Junita; Sarifah Nurjanah

    2017-01-01

    VCO contains of lauric acid that perform to smooth and to moisturize the skin. Castor oil has ricinoleic acid that serves to protect the skin. Therefore, both of the oil are suitable to be the raw material for liquid soap making. This study aimed were to produce a liquid soap, to determine the exact concentration of VCO and castor oil for liquid soap and to find out the effect of VCO and Castor Oil concentrations to the characteristics of liquid soap. The method used was laboratory experiment...

  13. Sorption of oil in the polyurethane from oil or castor oil; Sorcao de oleo no poliuretano derivado do petroleo ou do oleo de mamona

    Energy Technology Data Exchange (ETDEWEB)

    Florentino, Wagner M; Goulart, Shane A.S.; Mulinari, Daniella R. [Centro Universitario de Volta Redonda (UniFOA), Volta Redonda, RJ (Brazil)], e-mail: wagner-can@hotmail.com

    2011-07-01

    The oil outpouring has caused serious environmental problems. To minimize this damage polyurethane foams have been used. In this work, the use of polyurethane from oil and castor oil, in different polyol/diisocyanate mass ratios, to absorb oil was evaluated. Results revealed that the proportion of polyol and diisocyanate influenced in the sorption capacity and the polyurethane from castor oil presented greater sorption capacity. Of this way, can say that the polyurethane from oil can be replaced by castor oil, contributing to sustainable development. (author)

  14. Nuclear and Mitochondrial DNA Analyses of Golden Eagles (Aquila chrysaetos canadensis from Three Areas in Western North America; Initial Results and Conservation Implications.

    Directory of Open Access Journals (Sweden)

    Erica H Craig

    Full Text Available Understanding the genetics of a population is a critical component of developing conservation strategies. We used archived tissue samples from golden eagles (Aquila chrysaetos canadensis in three geographic regions of western North America to conduct a preliminary study of the genetics of the North American subspecies, and to provide data for United States Fish and Wildlife Service (USFWS decision-making for golden eagle management. We used a combination of mitochondrial DNA (mtDNA D-loop sequences and 16 nuclear DNA (nDNA microsatellite loci to investigate the extent of gene flow among our sampling areas in Idaho, California and Alaska and to determine if we could distinguish birds from the different geographic regions based on their genetic profiles. Our results indicate high genetic diversity, low genetic structure and high connectivity. Nuclear DNA Fst values between Idaho and California were low but significantly different from zero (0.026. Bayesian clustering methods indicated a single population, and we were unable to distinguish summer breeding residents from different regions. Results of the mtDNA AMOVA showed that most of the haplotype variation (97% was within the geographic populations while 3% variation was partitioned among them. One haplotype was common to all three areas. One region-specific haplotype was detected in California and one in Idaho, but additional sampling is required to determine if these haplotypes are unique to those geographic areas or a sampling artifact. We discuss potential sources of the high gene flow for this species including natal and breeding dispersal, floaters, and changes in migratory behavior as a result of environmental factors such as climate change and habitat alteration. Our preliminary findings can help inform the USFWS in development of golden eagle management strategies and provide a basis for additional research into the complex dynamics of the North American subspecies.

  15. Nuclear and Mitochondrial DNA Analyses of Golden Eagles (Aquila chrysaetos canadensis) from Three Areas in Western North America; Initial Results and Conservation Implications.

    Science.gov (United States)

    Craig, Erica H; Adams, Jennifer R; Waits, Lisette P; Fuller, Mark R; Whittington, Diana M

    2016-01-01

    Understanding the genetics of a population is a critical component of developing conservation strategies. We used archived tissue samples from golden eagles (Aquila chrysaetos canadensis) in three geographic regions of western North America to conduct a preliminary study of the genetics of the North American subspecies, and to provide data for United States Fish and Wildlife Service (USFWS) decision-making for golden eagle management. We used a combination of mitochondrial DNA (mtDNA) D-loop sequences and 16 nuclear DNA (nDNA) microsatellite loci to investigate the extent of gene flow among our sampling areas in Idaho, California and Alaska and to determine if we could distinguish birds from the different geographic regions based on their genetic profiles. Our results indicate high genetic diversity, low genetic structure and high connectivity. Nuclear DNA Fst values between Idaho and California were low but significantly different from zero (0.026). Bayesian clustering methods indicated a single population, and we were unable to distinguish summer breeding residents from different regions. Results of the mtDNA AMOVA showed that most of the haplotype variation (97%) was within the geographic populations while 3% variation was partitioned among them. One haplotype was common to all three areas. One region-specific haplotype was detected in California and one in Idaho, but additional sampling is required to determine if these haplotypes are unique to those geographic areas or a sampling artifact. We discuss potential sources of the high gene flow for this species including natal and breeding dispersal, floaters, and changes in migratory behavior as a result of environmental factors such as climate change and habitat alteration. Our preliminary findings can help inform the USFWS in development of golden eagle management strategies and provide a basis for additional research into the complex dynamics of the North American subspecies.

  16. Nuclear and mitochondrial DNA analyses of golden eagles (Aquila chrysaetos canadensis) from three areas in western North America; initial results and conservation implications

    Science.gov (United States)

    Craig, Erica H; Adams, Jennifer R.; Waits, Lisette P.; Fuller, Mark R.; Whittington, Diana M.

    2016-01-01

    Understanding the genetics of a population is a critical component of developing conservation strategies. We used archived tissue samples from golden eagles (Aquila chrysaetos canadensis) in three geographic regions of western North America to conduct a preliminary study of the genetics of the North American subspecies, and to provide data for United States Fish and Wildlife Service (USFWS) decision-making for golden eagle management. We used a combination of mitochondrial DNA (mtDNA) D-loop sequences and 16 nuclear DNA (nDNA) microsatellite loci to investigate the extent of gene flow among our sampling areas in Idaho, California and Alaska and to determine if we could distinguish birds from the different geographic regions based on their genetic profiles. Our results indicate high genetic diversity, low genetic structure and high connectivity. Nuclear DNA Fst values between Idaho and California were low but significantly different from zero (0.026). Bayesian clustering methods indicated a single population, and we were unable to distinguish summer breeding residents from different regions. Results of the mtDNA AMOVA showed that most of the haplotype variation (97%) was within the geographic populations while 3% variation was partitioned among them. One haplotype was common to all three areas. One region-specific haplotype was detected in California and one in Idaho, but additional sampling is required to determine if these haplotypes are unique to those geographic areas or a sampling artifact. We discuss potential sources of the high gene flow for this species including natal and breeding dispersal, floaters, and changes in migratory behavior as a result of environmental factors such as climate change and habitat alteration. Our preliminary findings can help inform the USFWS in development of golden eagle management strategies and provide a basis for additional research into the complex dynamics of the North American subspecies.

  17. Effects of Oganic and Biofertilizers on Growth Indices of Castor Bean (Ricinus communis L.

    Directory of Open Access Journals (Sweden)

    A Amin Ghafori

    2016-07-01

    Full Text Available Introduction Castor plant, Ricinus communis L. is a species of flowering plant in the spurge family; Euphorbiaceae, which contains a vast number of plants mostly native to the tropics. It belongs to a monotypic genus Ricinus. The name Ricinus is a latin word for tick. The plant is probably named because its seed has markings and a dump at the end that resemble certain ticks (NCRI, 2014. Castorbean is an industrial oil seed crop containing about 45-58 percent oil, which has tremendous application in petrochemicals, pharmaceuticals, cosmetics, textiles, chemicals, soap, leather, paints, varnishes, ink, nylon and plastic. Castor oil is traditionally associated with medicine and veterinary use in the fields of obstetrics, dermatology. It is also used as laxative. Presently, its utilization as bio-diesel has magnified its importance. Its oil does not freeze even at high altitudes and it is one the best lubricants for jet engines. This 100% castor-based product, has numerous applications in industry such as rotating glass car-wipers, ski boots fixatives, and for use in air-brake systems on trucks. Many new uses, based on the biodegradability of castor oil derived products, are expected in the future (Labalette et al., 1996. The shell of the castor bean is used as an organic termite control agent and its seed cake as manure in the soil. Medicinal plants are valuable resources in a wide range of natural resources that scientific identification, cultivation, development and proper utilization of them can have an important role in community health, employment and non-petrol exports. Quality of medicinal plants is more important than other crops. One of the most important factors determining the yield of castor bean is fertility. Integrated supply of nutrient to plants through combinations of organic and inorganic sources is becoming an increasingly important aspect of environmentally sound agriculture. Reports showed that the application of manure on bean

  18. Regional habitat needs of a nationally listed species, Canada Warbler (Cardellina canadensis, in Alberta, Canada

    Directory of Open Access Journals (Sweden)

    Jeffrey R. Ball

    2016-12-01

    Full Text Available Understanding factors that affect the distribution and abundance of species is critical to developing effective management plans for conservation. Our goal was to quantify the distribution and abundance of Canada Warbler (Cardellina canadensis, a threatened old-forest associate in Alberta, Canada. The Canada Warbler has declined across its range, including in Alberta where habitat loss and alteration from urban expansion, forestry, and energy development are changing the forest landscape. We used 110,427 point count survey visits from 32,287 unique survey stations to model local-level (150-m radius circular buffers and stand-level (564-m radius circular buffers habitat associations of the Canada Warbler. We found that habitat supporting higher densities of Canada Warblers was locally concentrated yet broadly distributed across Alberta's boreal forest region. Canada Warblers were most commonly associated with older deciduous forest at the local scale, particularly near small, incised streams, and greater amounts of deciduous forest at the stand scale. Predicted density was lower in other forest types and younger age classes measured at the local scale. There was little evidence that local-scale fragmentation (i.e., edges created by linear features influenced Canada Warbler abundance. However, current forestry practices in the province likely will reduce the availability of Canada Warbler habitat over time by cutting old deciduous forest stands. Our results suggest that conservation efforts aimed at Canada Warbler focus on retaining large stands of old deciduous forest, specifically stands adjacent to streams, by increasing the width of deciduous retention buffers along streams during harvest and increasing the size and number of old forest residual patches in harvested stands.

  19. Propagation of goldenrod (Solidago canadensis L. from leaf and nodal explants

    Directory of Open Access Journals (Sweden)

    Jun Li

    2012-02-01

    Full Text Available Goldenrod (Solidago canadensis L. is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog’s medium (MS medium supplemented with 1.0 mg/L naphthalene acetic acid (NAA and 1.0 mg/L 6-benzylaminopurine (BA. Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2 was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological varia­tion when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.

  20. Subtype-Specific Influenza A Virus Antibodies in Canada Geese (Branta canadensis)

    Science.gov (United States)

    Kistler, Whitney M.; Stallknecht, David E.; DeLiberto, Thomas J.; Van Why, Kyle; Yabsley, Michael J.

    2015-01-01

    Historically, surveillance for influenza A viruses (IAVs) in wild birds has relied on viral detection assays. This was largely due to poor performance of serological assays in wild birds; however, recently developed commercial serological assays have improved the ability to detect IAV antibodies in wild birds. Serological surveillance for IAV antibodies in Canada geese (Branta canadensis) has shown that, despite a low prevalence of virus isolations, Canada geese are frequently exposed to IAVs and that exposure increases with latitude, which follows virus isolation prevalence patterns observed in dabbling ducks. The objectives of this study were to further evaluate IAV antibodies in Canada geese using a subtype-specific serological assay to determine if Canada geese are exposed to subtypes that commonly circulate in dabbling ducks. We collected serum samples from Canada geese in Minnesota, New Jersey, Pennsylvania, and Wisconsin and tested for antibodies to IAVs using a blocking ELISA. Positive samples were further tested by hemagglutination inhibition for 10 hemagglutinin IAV subtypes (H1–H10). Overall, we detected antibodies to NP in 24% (714/2,919) of geese. Antibodies to H3, H4, H5, and H6 subtypes predominated, with H5 being detected most frequently. A decrease in H5 HI antibody prevalence and titers was observed from 2009 to 2012. We also detected similar exposure pattern in Canada geese from New Jersey, Minnesota, Washington and Wisconsin. Based on the published literature, H3, H4, and H6 viruses are the most commonly reported IAVs from dabbling ducks. These results indicate that Canada geese also are frequently exposed to viruses of the same HA subtypes; however, the high prevalence of antibodies to H5 viruses was not expected as H5 IAVs are generally not well represented in reported isolates from ducks. PMID:25845755