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Sample records for castaneum herbst coleoptera

  1. Efeitos da radiação gama do Cobalto-60 em ovos de Tribolium castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae) Gamma radiation effects on eggs of Tribolium castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae)

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    L. S. Fontes; V. Arthur

    1994-01-01

    Este trabalho teve por objetivo determinar as doses esterilizantes e letal para ovos de T. castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae), através de doses crescentes de radiação gama. Utilizou-se uma fonte de Cobalto-60, tipo Gammabeam-650, com taxa de dose de 1,28 kGy/hora. O experimento foi conduzido sob condições controladas com temperatura de 25 ± 2°C e umidade relativa de 70 ± 5%. As doses letal e esterilizante para os ovos foram respectivamente 30 e 20 Gy.The o...

  2. Gamma radiation effects of Cobalt-60 on eggs of Tribolium castaneum (Herbst., 1797) (Coleoptera: tenebrionidae)

    International Nuclear Information System (INIS)

    The objective of this research was to verify the effects of gamma radiation of a cobalt-60 source on eggs of the red flour beetle Tribolium castaneum (Herbst., 1797). The used dose rate was 1.28 kGy per hour, and the irradiated insects were kept under controlled environmental conditions: 25 ± 20 C and 70 ± 5% relative humidity. (author). 11 refs., 2 figs., 1 tab

  3. Efeitos da radiação gama do Cobalto-60 em ovos de Tribolium castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae Gamma radiation effects on eggs of Tribolium castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae

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    L. S. Fontes

    1994-12-01

    Full Text Available Este trabalho teve por objetivo determinar as doses esterilizantes e letal para ovos de T. castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae, através de doses crescentes de radiação gama. Utilizou-se uma fonte de Cobalto-60, tipo Gammabeam-650, com taxa de dose de 1,28 kGy/hora. O experimento foi conduzido sob condições controladas com temperatura de 25 ± 2°C e umidade relativa de 70 ± 5%. As doses letal e esterilizante para os ovos foram respectivamente 30 e 20 Gy.The objective of this research was to verify the effects of gamma radiation of a Cobalt-60 source on eggs of the red flour beetle Tribolium castaneum (Herbst., 1797. The used dose rate was 1.28 kGy per hour, and the irradiated insects were kept under controlled environment condition: 25 ± 2°C and 70 ± 5% relative humidity. For eggs the sterilizing and lethal dose were respectively, 20 and 30 Gy.

  4. Acute, Lethal and Synergistic Effects of Some Terpenes Against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae

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    M. K. Chaubey

    2012-07-01

    Full Text Available Terpenes are found in abundance in all plant essential oils. In the present study two pure volatile compounds of terpene group, α-pinene and β-caryophyllene have been evaluated for their repellent, acute toxicity and developmental inhibitory activities alone and in binary combination against red flour beetle Tribolium castaneum. In repellency assay, α-pinene and β-caryophyllene repelled T. castaneum adults significantly even at 0.025% concentrations. Fumigation of larvae and adults of T. castaneum with these two compounds caused lethality in them. Median lethal concentrations (LC50 of α-pinene and β-caryophyllene against adults were 0.998 and 1.624 μl/cm3 and against larvae were 1.379 and 1.949 μl/cm3 respectively. In binary combination, the LC50 values against adults and larvae were found 1.277 and 1.438 μl/cm3 respectively. Fumigation with two sublethal concentrations viz. 40 and 80% of 24-h LC50 of these two compunds alone and in binary combination significantly reduced oviposition potential of adults and inhibited pupation and adult emergence in larvae. All the responses were found concentration-dependent. From the present study, it can be concluded that α-pinene is more active than β-caryophyllene and these two volatile compounds in binary combination shows synergism and thus, can used as efficient insecticidal tool against T. castaneum as fumigant either alone or in combination.

  5. Differential preference of colored surface in Tribolium castaneum (Herbst

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    AMS Reza

    2006-07-01

    Full Text Available Insects show color preferences mostly to those which resembles the color of foliage, flower or even their hosts. In the present study observations were made to determine vision towards different colored surfaces in young (second instar, and advanced (fourth instar larvae, and adults of Tribolium castaneum (Herbst (Coleoptera: Tenebrionidae. The larva and adult beetles showed significant color preferences when given a choice between white (control and colored surfaces at 24- and 48-h exposures. The second instar larvae were more attracted by yellow and pink than green surfaces. The fourth instar larvae did not show any significant preference between white and colored surfaces at 24-h exposure, but avoided red and pink surfaces (P>0.05 and had a marginal choice for black (P<0.05. The adults avoided green significantly at both exposure times and pink at 48-h exposure, but was moderately attracted by black (P<0.05 at both exposure times.

  6. Evaluación de la actividad repelente e insecticida de aceites esenciales extraídos de plantas aromáticas utilizados contra tribolium castaneum herbst (coleoptera: tenebrionidae)

    OpenAIRE

    Espitia Yanes, Carmen Regina

    2011-01-01

    Los aceites esenciales son mezclas volátiles de terpenos obtenidas de plantas. Estos productos poseen propiedades repelentes e insecticida de insectos. Uno de los insectos de mayor importancia económica es el Tribolium castaneum Herbst. Con el objetivo de evaluar la actividad de aceites esenciales de plantas de Colombia Cymbopogon citratus y (GRbgV02E), Lippia origanoides (VEbgV07E) y Eucalyptus citriodora (MYbgV01E) fueron realizados ensayos de repelencia y mortalidad. Para tal efecto, fué u...

  7. Gamma radiation effects on pupae of Tribolium castaneum (HERBST)

    International Nuclear Information System (INIS)

    The objective of the present paper was to determine the gamma radiation dose able to sterilize adults of the red flour beetle, Tribolium castaneum (Herbst, 1797) if irradiated in its pupal stage. After irradiation, with the doses of 25, 50, 75, 100, 125 and 150 Gy, at a dose rate of 1.24 kGy per hour, the insects were maintained under controlled environmental conditions, at temperatures between 23 deg and 27 deg C and relative humidity between 65 and 75 percent. It was observed that the sterilization of the adults was achieved with the irradiation of its pupae by the dose of 50 Gy, and the lethal dose to 85 percent of the population was 150 Gy with immediate kill. (author). 12 refs., 1 tab

  8. Pesticidal Evaluation of Manilkara zapota (L. against Tribolium castaneum (Herbst

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    Muhammad Abu Osman

    2011-06-01

    Full Text Available This paper presents the residual eff ects of Manilkara zapota (L. P. Royen plant against the red flour beetle, Tribolium castaneum (Herbst. Four doses, i.e., 1238.5, 619.25, 309.6 and 154.8 μg/cm2 of ethyl acetate extract of stem bark of Manilkara zapota were applied on larvae and adult beetles. The effectiveness of the plant extract was increased with the increase of exposure time and after 72 hrs of exposure, the maximum residual toxicity was observed with LD50 of 228.8, 281.1, 413.4, 423.7, 455.2, 498.7 and 526.5 μg/cm2 for first, second, third, fourth, fifth, sixth instar larvae and adults, respectively. The results of this study also demonstrated that the earlier instars were more sensitive to the extracts than those of late instars as well as adults.

  9. Pesticidal Evaluation of Manilkara zapota (L. against Tribolium castaneum (Herbst

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    Muhammad Abu Osman

    2014-02-01

    Full Text Available This paper presents the residual eff ects of Manilkara zapota (L. P. Royen plant against the red flour beetle, Tribolium castaneum (Herbst. Four doses, i.e., 1238.5, 619.25, 309.6 and 154.8 μg/cm2 of ethyl acetate extract of stem bark of Manilkara zapota were applied on larvae and adult beetles. The effectiveness of the plant extract was increased with the increase of exposure time and after 72 hrs of exposure, the maximum residual toxicity was observed with LD50 of 228.8, 281.1, 413.4, 423.7, 455.2, 498.7 and 526.5 μg/cm2 for first, second, third, fourth, fifth, sixth instar larvae and adults, respectively. The results of this study also demonstrated that the earlier instars were more sensitive to the extracts than those of late instars as well as adults.

  10. 七种糕点对赤拟谷盗(鞘翅目:拟步甲科)感染的抗性%Resistance of Seven Biscuit Types to Infestation by Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

    Institute of Scientific and Technical Information of China (English)

    Olusola O. ODEYEMI; Bernice M. OYEDARE; Michael O. ASHAMO

    2005-01-01

    Seven biscuit types namely, Okin, Digestive, Cabin, Peanut, Cream crackers, Hobnobs and Glucose and wheat flour were screened for their resistance to Tribolium castaneum (Herbst) at ambient temperature of 28±2 ℃ and 78±2% relative humidity in the laboratory. 50 g of each biscuit sample were infested with four female and two male adult beetles and left for 15 days then remove for a resistant experiment and 70 days for a mortality experiment. Each treatment and the control without beetles were replicated three times. Results showed that there was significant difference (P0.05) in the weight loss of biscuit types. The highest adult mortality of beetles (100%) was obtained from Peanut biscuits at 28 days after infestation while there was only 5.5% adult mortality in wheat flour at 70 days after infestation. The susceptibility index was 0 for all the biscuit types since there was no adult emergence indicating that they were resistant to T. castaneum infestation. Resistance in the biscuit types could be due to chemical additives (e.g. sodium chloride and sodium bicarbonate) used in the production of biscuits, which may have inhibitory effect on the development of T. castaneum and also the low moisture content of the biscuits. Packages that can easily be perforated or damaged to allow absorption of moisture from the environment should not be used in packing biscuits.

  11. NSECTICIDAL TOXICITY OF 1,8-CINEOLE, CAMPHOR AND EUGENOL ON TRIBOLIUM CASTANEUM (HERBST)

    OpenAIRE

    Anita Liška

    2011-01-01

    Contact and fumigant activity of 1,8-cineole, camphor and eugenol compounds were tested In laboratory conditions on adult, larvae and pupae of the red flour beetle Tribolium castaneum (Herbst), as well as their effect on progeny emergence. Positive results were obtained with contact application for all three tested compounds and on all three stages of development of T. castaneum, with the highest activity of 1,8-cineole, followed by eugenol and camphor. In general, fumigant activity of all th...

  12. INSECTICIDAL TOXICITY OF 1,8-CINEOLE, CAMPHOR AND EUGENOL ON TRIBOLIUM CASTANEUM (HERBST)

    OpenAIRE

    Anita Liška

    2011-01-01

    Contact and fumigant activity of 1,8-cineole, camphor and eugenol compounds were tested In laboratory conditions on adult, larvae and pupae of the red flour beetle Tribolium castaneum (Herbst), as well as their effect on progeny emergence. Positive results were obtained with contact application for all three tested compounds and on all three stages of development of T. castaneum, with the highest activity of 1,8-cineole, followed by eugenol and camphor. Ingeneral, fumi...

  13. Toxicity of Boldo Peumus boldus Molina for Sitophilus zeamais Motschulsky and Tribolium castaneum Herbst Toxicidad del Boldo, Peumus boldus Molina, sobre Sitophilus zeamais Motschulsky y Tribolium castaneum Herbst

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    Margarita Ortiz U

    2012-09-01

    Full Text Available The maize weevil (Sitophilus zeamais Motschulsky and the red flour beetle (Tribolium castaneum Herbst are two key pests of stored-grain products worldwide. The insecticidal activity of boldo (Peumus boldus Molina powder, liquid ethanolic and hexanic extracts against S. zeamais and T. castaneum were evaluated under laboratory conditions. The evaluated variables were mortality, emergence of adult insects (F1, and grain weight loss. The experimental design was completely randomized. The mortality in S. zeamais was 100% even at the lowest powder concentration (0.5% w/w, whereas emergence of F1 adult insects was 0% and grain weight loss was El gorgojo del maíz (Sitophilus zeamais Motschulsky y el gorgojo castaño de la harina (Tribolium castaneum Herbst son plagas primarias de productos almacenados a nivel mundial. Se evaluó en laboratorio la actividad insecticida de polvo y extractos líquidos etanólicos y hexánicos del boldo (Peumus boldus Molina sobre S. zeamais y T. castaneum. Las variables evaluadas fueron mortalidad y emergencia de insectos adultos (F1 y pérdida de peso de los granos con un diseño experimental completamente al azar. La mortalidad en S. zeamais fue 100%, incluso con la concentración menor (0,5% p/p mientras que la emergencia de insectos adultos y la pérdida de peso de granos de maíz fue < 0,08%. Para T. castaneum sólo las concentraciones de 8 y 16% p/p de polvo causaron una mortalidad de 100%. Los extractos en agua, etanol, y hexano tuvieron un efecto insecticida de 100% en S. zeamais, mientras que en T. castaneum sólo el extracto en etanol alcanzó este valor. Por lo tanto, el polvo y los extractos evaluados de P. boldus presentan actividad insecticida contra S. zeamais y T. castaneum y son promisorios para utilizarse contra éstas y otras plagas de granos almacenados.

  14. Effect of gamma irradiation on mortality of tribolium castaneum (HERBST)

    International Nuclear Information System (INIS)

    An investigation was made to assess the susceptibility of larvae and adults of Tribolium castaneum to gamma irradiation. The larvae were more susceptible to irradiation than the adults. A dose of 0.05 kGy killed all the larvae but higher dose of 0.15 kGy was required to kill all the adults. Therefore, a dose of 0.15 kGy was found to be the effective dose to kill both the larvae and the adults of T. castaneum. (author)

  15. Laboratory evaluation of some indigenous plant extracts as toxicants against red flour beetle, Tribolium castaneum Herbst

    OpenAIRE

    Mamum, M.S.A; Shahjahan, M.; Ahmad, M.

    2009-01-01

    Experiments were carried out to evaluate the toxicity of six botanicals, Bazna (Zanthoxylum rhetsa), Ghora-neem (Melia sempervirens), Hijal (Barringtonia acutangula), Karanja (Pongamia pinnata), Mahogoni (Swietenia mahagoni) and Neem (Azadirachta indica) against red flour beetle, Tribolium castaneum Herbst. Leaf and seed extracts were prepared by using acetone, methanol and water as solvents. The results showed that extracts of all the six plants had direct toxic effect on red flour beetle. A...

  16. BIOACTIVITY OF 1,8-CINEOLE AGAINST RED FLOUR BEETLE TRIBOLIUM CASTANEUM (HERBST

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    Anita Liška

    2011-06-01

    Full Text Available Red flour beetle Tribolium castaneum (Herbst is a major pest of stored products. The aim of this study was to assess the potential fumigant effects of 1,8-cineole, essential oil component, on the T. castaneum pupae. The compound was tested in 6 doses; in two treatments (fumigation without grain and with wheat grain, exposed for 48 h, in 4 repetitions, for each gender. The compound 1,8-cineole had lethal effect on the treated pupae at both genders and in the both treatments. Total proportion of the normally developed beetles was decreased. In addition, 1,8-cineole had also a growth regulator effect, producing adultoids and deformed units, with males more susceptible. In the treatment with the grain there were significant lower dead pupae, normally developed live male beetles and also deformed female units in the stage 2. In general, compound 1,8-cineole has multiple effect against T. castaneum in pupal stage.

  17. EFFECTS OF ESSENTIAL OIL FORMULATIONS ON THE ADULT INSECT TRIBOLIUM CASTANEUM (HERBST) (COL., TENEBRIONIDAE)

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    Aleksandra Popovic; Jovana Sucur; Dejan Orcic; Pero Strbac

    2013-01-01

    Stored product pests such as Tribolium castaneum ( Herbst, 1979) are a major problem. Adult insects were obtained from laboratory cultures maintained in the dark in incubators at 25 1C and 70 80percent r.h., reared on wheat flour and fed with flour disks containing a known concentration of essential oil of 9 plants. The chemical components of essential oil of 3 plants, collected on the area of Montenegro, were also identified using GC-MS analysis. The results of insecticidal effect of essenti...

  18. Biochemical abnormalities induced by abamectin in sixth instar larvae of the red flour beetle, tribolium castaneum (herbst)

    International Nuclear Information System (INIS)

    The sub lethal effects of abamectin (Sure 1.8 EC) were studied on malathion-resistant (PAK) and organophosphate susceptible (FSS-II) strains of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) larvae in the laboratory. The objective was to examine changes in production or activities of carboxylesterase (CE), total esterases (TE), alpha-amylase, glucoamylase, alkaline phosphatase (AkP), acidic phosphatase (AcP), total protein, soluble protein and free amino acids (FAA). The sixth instar larvae of T. castaneum were released and exposed for 48h without food on abamectin treated glass petri dishes. The surviving ones were then homogenized in saline and centrifuged prior to biochemical analyses. Results showed differences in the activities of enzymes and quantities of total protein, soluble protein and FAA between strains and among concentrations. Abamectin, at LC and LC , changed the activities 10 20/levels of TE, CE, AcP, total protein and FAA in the larvae of both the strains. The activities of alpha-amylase, glucoamylase and AkP remained non-significant at both doses in the two strains. In PAK strain larvae, the TE activity was inhibited with depletion of total protein contents and elevation of FAA contents. In FSS-II larvae, the effect of abamectin on levels of alpha-amylase, glucoamylase, AkP, total protein and soluble protein remained non-significant. The activities of TE and AcP were reduced at both doses, while activities/levels of CE reduced at LC and FAA increased 10 at LC . It is concluded that abamectin affected the overall body 20 functioning of PAK strain more as compared to FSS-II strain considering disturbances caused in the levels/activities of biochemical components. (author)

  19. EFFECTS OF ESSENTIAL OIL FORMULATIONS ON THE ADULT INSECT TRIBOLIUM CASTANEUM (HERBST (COL., TENEBRIONIDAE

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    Aleksandra Popovic

    2013-06-01

    Full Text Available Stored product pests such as Tribolium castaneum ( Herbst, 1979 are a major problem. Adult insects were obtained from laboratory cultures maintained in the dark in incubators at 25 1C and 70 80percent r.h., reared on wheat flour and fed with flour disks containing a known concentration of essential oil of 9 plants. The chemical components of essential oil of 3 plants, collected on the area of Montenegro, were also identified using GC-MS analysis. The results of insecticidal effect of essential oils were discussed. Also, mortality rate of adult insects was tested. In this research, the essential oils of C. glandulosa which were rich in monoterpene alcohols carvacrol and contained ketonic component showed strong insecticidal and fumigant activity against adults of T. castaneum. Less toxic effect showed essential oils of Satureja montana which had a lower carvacrol and ketonic content. On the other hand, essential oils of Teucrium polium which did not contain ketonic component did not show any activity. Therefore, it was observed that essential oils of C. glandulosa with concentration of 1.14% showed powerful toxic and repellent effect, with very high mortality rate after 24h (56,67%.

  20. Toxicities of azadirachtin and polychlorinated petroleum Hydrocarbon against resist and susceptible strains of tribolium castaneum (coleoptera: tenebrionidae) adults

    International Nuclear Information System (INIS)

    The LC/sub 50/ values for malathion-resistant (PAK) and organo-50 phosphate-susceptible (FSS-II) strains of red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) adult beetles were determined through filter paper impregnated method against azadirachtin (Nimbokil 60 EC) and polychlorinated petroleum hydrocarbon (Tenekil 100 EC). The LC values of these insecticides were worked out as 12830 and 50 9331 ppm for azadirachtin and 5148 and 4047 ppm for Tenekil 100 EC against PAK and FSS-II strains, respectively. The results revealed that polychlorinated petroleum hydrocarbon was more toxic than the azadirachtin. Furthermore, both the insecticides were equally toxic to the adult beetles of T. castaneum as the difference was non-significant because of overlapping 95% FLs to LC./sub 50/. (author)

  1. Toxicity of several contact insecticides to Tribolium castaneum (Herbst) populations after selection with pirimiphos-methyl and deltamethrin

    OpenAIRE

    Andrić Goran; Pražić-Golić Marijana; Kljajić Petar

    2015-01-01

    Laboratory bioassays were conducted to detect possible alteration in susceptibility of two field Tribolium castaneum (Herbst) populations (sampled in a warehouse in Nikinci and a silo in Jakovo) to dichlorvos, malathion, chlorpyrifos-methyl, pirimiphos-methyl, deltamethrin and bifenthrin after previous selection with the LD80 of pirimiphos-methyl and deltamethrin. Data from the topical application bioassays show that chlorpyrifos-methyl was the most toxic i...

  2. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae

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    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.

  3. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae

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    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae. A atividade inseticida de Toona sureni (Blume Merr. foi avaliada considerando repelência, mortalidade e a produção de progênie de adultos F1 de Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Extrato seco de sementes

  4. Penetration of 14C-labelled methyl parathion in Tribolium castaneum (Herbst)

    International Nuclear Information System (INIS)

    Studies on the rate of penetration of radioactive methyl parathion (14C-ri ng labelled) were conducted under laboratory conditions, by applying 5 ng toxicant in 1μl acetone/insect on the thoracic sternum of rust red flour beetle, Tribolium castaneum (Herbst). Results revealed that the penetration of this toxicant was non-linear with two distinct phases. The earlier phase was faster and lasted upto 60 min from the time of application (half-life:14±3 min) followed by a slower phase from 60 min to 24 hr with longer half-life (909± 313 min). It was further observed that at the end of the faster phase i.e., 1 hr after treatment, nearly 68 per cent of the applied insecticide was absorbed by the beetle whereas the maximum quantity of the toxicant absorbed by the insect at the end of the experiment (24 hr after treatment) was 89 per cent. (author). 27 refs

  5. Life History of the Tamarind Weevil, Sitophilus linearis (Herbst) (Coleoptera: Curculionidae), on Tamarind Seed

    OpenAIRE

    James Adebayo Ojo; Adebayo Amos Omoloye

    2015-01-01

    The tamarind weevil, Sitophilus linearis Herbst (Coleoptera: Curculionidae), is an important pest of tamarind and other Caesalpinioideae. Investigating its life history is important in the implementation of management strategy. Its life history was monitored daily to understand its developmental biology on tamarind seed following standard procedures under laboratory conditions of 24–30°C temperature, 60–70% relative humidity, and 12L : 12D photoperiod. The egg incubation period lasted 3.17 ± ...

  6. Age and sex related responsiveness of Tribolium castaneum (Coleoptera: Tenebrionidae) in novel behavioral bioassays

    Science.gov (United States)

    The hardiness and mobile nature of Tribolium castaneum (Herbst) make them easy to work with but are the same factors that make their responses to behavior modifying chemical stimuli difficult to evaluate. To overcome these difficulties we developed two bioassays: a two choice test with airflow and a...

  7. Toxicity of several contact insecticides to Tribolium castaneum (Herbst populations after selection with pirimiphos-methyl and deltamethrin

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    Goran Andrić

    2015-12-01

    Full Text Available Laboratory bioassays were conducted to detect possible alteration in susceptibility of two field Tribolium castaneum (Herbst populations (sampled in a warehouse in Nikinci and a silo in Jakovo to dichlorvos, malathion, chlorpyrifos-methyl, pirimiphos-methyl, deltamethrin and bifenthrin after previous selection with the LD80 of pirimiphos-methyl and deltamethrin. Data from the topical application bioassays show that chlorpyrifos-methyl was the most toxic insecticide to T. castaneum adults of the Nikinci population selected with pirimiphosmethyl and deltamethrin, while malathion was the weakest, and both selection procedures changed/reduced significantly only the toxicity of deltamethrin and bifenthrin, increasing their resistance ratios (RR at the LD50 from 1.1 to 1.8 (bifenthrin and from 0.9 to 2.2 (deltamethrin. Deltamethrin was the most toxic insecticide for Jakovo adults selected with the LD80 of pirimiphosmethyl, while malathion was again the least toxic. Selection of that population had no effect on insecticide toxicity, except of malathion, which had a rise in RR at the LD50 from 26.0 to 29.8.

  8. Insecticidal activity of Ageratum conyzoides L., Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit essential oils as fumigant against storage grain insect Tribolium castaneum Herbst

    OpenAIRE

    Jaya; Singh, Priyanka; Prakash, Bhanu; Dubey, N.K.

    2012-01-01

    Essential oils (EOs) from Ageratum conyzoides L., Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit were extracted and tested against Tribolium castaneum Herbst, the storage grain insect. The EOs were found effective against Tribolium castaneum during in vitro as well as in vivo fumigant testing. The EOs of H. suaveolens and A. conyzoides showed 100 % mortality of test insect at 250 ppm while C. aromaticus at 350 ppm. During in vivo fumigant testing of wheat samples against Tribolium c...

  9. PATCH EXPLOITATION BY FEMALE RED FLOUR BEETLES, TRIBOLIUM CASTANEUM

    Science.gov (United States)

    The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera:Tenebrionidae) has had a long association with human stored food and can be a major pest in anthropogenic structures used for the processing and storage of grain-based products. Anthropogenic structures are fragmented landscapes characte...

  10. An appraisal of resistance of old and new wheat genotypes to red flour beetle (tribolium castaneum herbst)

    International Nuclear Information System (INIS)

    In the present investigation, 30 varieties of wheat were used to study their resistance against Red flour beetle, Tribolium castaneum (Herbst). The grains of all these varieties were obtained from Plant Breeding and Genetics Division, Nuclear and 65 +- 5% R.H. The standard of Institute of Agriculture, Tandojam. Experiment was conducted in the laboratory at 29 +- 2 deg. C. the samples of each variety used in the experiment was 1000 grains, kept in plastic jars (15 x 6 cm). Ten pairs of newly emerged adult flour beetles (both sexes in equivalent numbers) of uniform age from laboratory stocked culture were released in each jar. The results were evaluated on the bases of adult population development, percent grain damage and frass production, revealed that none of the genotype was completely resistant to the infestation of T. castaneum. All the varieties suffered losses, but their degree of resistance varied significantly. On the bases of pest population development, percent damage and frass production, the least damage was noted in variety Barani-70 and Bhittai and the highest damage was and T; hence these varieties were designated the most tolerant and the most susceptible varieties, recorded in T/sub 21/ and T/sub 16/ respectively. The comparative resistance displayed by the wheat varieties, could be placed in the following order: < Barani- 70< Bhatti < T/sub 19/ < T/sub 14/ < T/sub 24/ < T/sub 13/ < T/sub 20/ < T/sub 9/ < T/sub 11/ < T/sub 15/ < T/sub 25/ < T/sub 1/ < T/sub 25/ < T.J.-83 < T/sub 22/ < T/sub 3/ < T/sub 18/< T/sub 10/ < T/sub 23/ < T/sub 10/ < T/sub 23/. (author)

  11. Repellent activity of essential oils and some of their individual constituents against Tribolium castaneum herbst.

    Science.gov (United States)

    Caballero-Gallardo, Karina; Olivero-Verbel, Jesús; Stashenko, Elena E

    2011-03-01

    A tool for integrated pest management is the use of essential oils (EOs) and plant extracts. In this study, EOs from Tagetes lucida , Lepechinia betonicifolia , Lippia alba , Cananga odorata , and Rosmarinus officinalis , species grown in Colombia, were analyzed by gas chromatography-mass spectrometry. These oils as well as several of their constituents were tested for repellent activity against Tribolium castaneum , using the area preference method. The main components (>10%) found in EOs were methylchavicol, limonene/α-pinene, carvone/limonene, benzyl acetate/linalool/benzyl benzoate, and α-pinene, for T. lucida, L. betonicifolia, L. alba, C. odorata, and R. officinalis, respectively. All EOs were repellent, followed a dose-response relationship, and had bioactivity similar to or better than that of commercial compound IR3535. EOs from C. odorata and L. alba were the most active. Compounds from EOs, such benzyl benzoate, β-myrcene, and carvone, showed good repellent properties. In short, EOs from plants cultivated in Colombia are sources of repellents against T. castaneum. PMID:21291237

  12. Efficacy of chlorfenapyr against Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) adults exposed on concrete, vinyl tile, and plywood surfaces

    Science.gov (United States)

    The insecticidal pyrrol chlorfenapyr was applied to concrete, tile, and wood surfaces, at an application rate of 0.11 mgAI/cm2. Adult Tribolium castaneum (Herbst), the red flour beetle, and adult T. confusum (DuVal), the confused flour beetle, were exposed for 2 and 4 hours, removed, and held witho...

  13. Actividad insecticida del aceite esencial de Lippia alba (Mill.) N. E. Brown sobre Tribolium castaneum Herbst. en granos de trigo (Triticum aestivum L.)

    OpenAIRE

    Ringuelet, Jorge Abel; Ocampo, Rafael; Henning, Cynthia; Padín, Susana; Urrutia, María Inés; Dal Bello, Gustavo

    2014-01-01

    Tribolium castaneum (Coleoptera: Tenebrionidae) es un insecto plaga de granos, muy destructivo en la etapa de almacenamiento y altamente resistente a los fitosanitarios sintéticos. Por esta razón su control químico se realiza con altas concentraciones de insecticidas que aumentan la contaminación ambiental y los peligros para la salud humana. Las tendências actuales en el manejo integrado de plagas se orientan hacia el uso de plaguicidas biológicos o biopesticidas como los extractos vegetales...

  14. Chemical composition of four essential oils from Eupatorium spp: Biological activities toward Tribolium castaneum (Coleoptera: Tenebrionidae) Composición química de cuatro aceites esenciales provenientes de Eupatorium spp. y su toxicidad para Tribolium castaneum (Coleoptera: Tenebrionidae)

    OpenAIRE

    Hugo G. Lancelle; Oscar S. Giordano; Marta E. Sosa; CARLOS E TONN

    2009-01-01

    Toxic and repellent properties of whole essential oils from four Eupatorium (Asteraceae) species (E. buniifolium Hook. et Arn, E. inulaefolium Kunth, E. arnottii Baker, and E. viscidum Hook. & Arn) were investigated in different concentrations toward Tribolium castaneum Herbst adults. The essential oils were isolated by hydrodistillation techniques from the aerial parts. The analysis was performed by GC-FID and GC-MS methods. Contact toxicity assays showed that all the evaluated essential oil...

  15. Evaluación de la acción insecticida de la rapanona sobre Tribolium castaneum (Herbst

    Directory of Open Access Journals (Sweden)

    Chavez Germán

    1992-11-01

    Full Text Available Experiments were carried out to detect and estimate the insect-killing effect of rapanone on the flour beetle  T.castaneum. Two kinds ofessays were made: by ingestion and by contacto It was proved that rapanone has insecticide activity on T. castaneum. Relatively high LD50 were determined, in comprarison
    with those of conventional commercial insecticides. An inverse proportionality, between doses of rapanone and the mean productivity, was also found; adose of 7000 mg rapanone per kg standard culture medium is enough to exterminate, almost completely, a population of T. castaneum in one generation.
    Se realizaron experimentos para detectar y cuantificar efectos insecticidas de la rapa nona sobre el escarabajo de la harina, Tribolium castaneum. Las pruebas fueron de dos clases: por ingestión y por contacto. Se comprobó que la rapanona tiene propiedades insecticidas sobre T. castaneum. Las toxicidades agudas, medidas en términos de DL50 fueron relativamente altas comparadas con insecticidas sintéticos comerciales. También se encontró una proporcionalidad inversa entre dosis de rapanona y productividad promedio; una dosis de 7000 mg de rapanona por kilo de alimento, es suficiente para exterminar casi del todo una poblaci6n de T.castaneum en una sola generación.

  16. The Growth of Tribolium castaneum (Herbst) and Lasioderma serricorne (Fabricius) on Feed Media Dosed with Flavour Volatiles Found in Dry Cocoa Beans

    OpenAIRE

    Jonfia-Essien, W. A.; Alderson, P. G.; G. Tucker; R. Linforth; West, G.

    2007-01-01

    Acetophenone, ethyl butyrate and 2-phenyl ethanol were used as additives to media on which Tribolium castaneum (Herbst) and Lasioderma serricorne (Fabricius) were cultured for 65 days. Acetophenone and ethyl butyrate had a positive impact on the weight and multiplication of the insects. The weight and rate of multiplication was higher in feed media containing acetophenone alone. The amount of the flavour volatiles added was high enough to deter the Lasioderma from feeding well, which affected...

  17. Insecticidal properties of essential oils against Tribolium castaneum (Herbst) and their inhibitory effects on acetylcholinesterase and adenosine triphosphatases.

    Science.gov (United States)

    Abou-Taleb, Hamdy K; Mohamed, Magdy I E; Shawir, Mohamed S; Abdelgaleil, Samir A M

    2016-01-01

    Essential oils from 20 Egyptian plants were obtained by using hydrodistillation. The chemical composition of the isolated oils was identified by gas chromatograph/mass spectrometer. Fumigant and contact toxicities of the essential oils were evaluated against the adults of Tribolium castaneum. In fumigation assays, the oil of Origanum vulgare (LC50 = 9.97 mg/L air) displayed the highest toxicity towards the adults of T. castaneum. In contact assays, the oils of Artemisia monosperma (LC50 = 0.07 mg/cm(2)) and O. vulgare (LC50 = 0.07 mg/cm(2)) were the most potent toxicants against the adults of T. castaneum. Biochemical studies showed that the tested oils caused pronounced inhibition of acetylcholinesterase (AChE) and adenosine triphosphatases (ATPases) isolated from the larvae of T. castaneum. The oil Cupressus macrocarpa (IC50 = 12.3 mg/L) was the most potent inhibitor of AChE, while the oil of Calistemon viminals (IC50 = 4.4 mg/L) was the most potent inhibitor of ATPases. PMID:25978134

  18. Chemical constituents and biological activities against Tribolium castaneum (Herbst of the essential oil from Citrus wilsonii leaves

    Directory of Open Access Journals (Sweden)

    Chen Hai Ping

    2014-01-01

    Full Text Available The essential oil obtained from Citrus wilsonii Tanaka leaves with hydrodistillation was investigated by GC and GC-MS. The main components of the essential oil were identified to be citronellol (16.94%, nerol acetate (10.35%, γ-terpinen (9.85%, citronellal (9.36% and β-pinene (6.72%. Among them, the four active constituents, predicted with a bioactivity-test, were isolated and identified as citronellol, γ-terpinene, nerol (neryl acetate and β-pinene. It was found that the essential oil of C. wilsonii leaves and isolated compounds possessed fumigant and contact toxicity against Tribolium castaneum adults. The essential oil and γ-terpinen showed strong fumigant toxicity against T. castaneum (LC50 = 8.18 and 4.09 mg L-1. Repellency of the crude oil and active compounds was also determined. Citronellol, neryl acetate and β-pinene were strongly repellent (100%, 86% and 92%, respectively, at 78.63 nL cm-2, after 2 h treatment against T. castaneum. The essential oil and citronellol exhibited the same level of repellency compared with the positive control, DEET. The results indicate that the essential oil of C. wilsonii leaves and its active compounds had the potential to be developed as natural fumigants, insecticides and repellents for control of T. castaneum.

  19. Residual efficacy of methoprene for control of Tribolium castaneum (Coleoptera: Tenebrionidae) larvae at different temperatures on varnished wood, concrete, and wheat.

    Science.gov (United States)

    Wijayaratne, L K Wolly; Fields, Paul G; Arthur, Frank H

    2012-04-01

    The residual efficacy of the juvenile hormone analog methoprene (Diacon II) was evaluated in bioassays using larvae of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) exposed on unsealed concrete or varnished wood treated with a liquid formulation and held at different temperatures. When these two types of surfaces were stored at 20, 30 or 35 degrees C for 0-24 wk, the percentage of adult emergence on concrete increased with time. In contrast, there was no adult emergence from larvae exposed to varnished wood at 24 wk after treatment at any of these temperatures. The presence of flour reduced residual efficacy of methoprene on concrete, but not on varnished wood, with no differences between cleaning frequencies. Methoprene was also stable for 48 h on concrete held at 65 degrees C and wheat, Triticum aestivum L., held at 46 degrees C. Results show that methoprene is stable at a range of temperatures commonly encountered in indoor food storage facilities and at high temperatures attained during insecticidal heat treatments of structures. The residual persistence of methoprene applied to different surface substrates may be affected more by the substrate than by temperature. PMID:22606845

  20. Effect of five diatomaceous earth formulations against Tribolium castaneum (Coleoptera: Tenebrionidae),Oryzaephilus surinamensis (Coleoptera: Silvanidae) and Rhyzopertha dominica (Coleoptera: Bostrychidae)

    Institute of Scientific and Technical Information of China (English)

    MASUMEH ZIAEE; ADEL KHASHAVEH

    2007-01-01

    Laboratory bioassays were conducted to determine the effect of food source on the survival of Tribolium castaneum Herbst, Oryzaephilus surinamensis L. and Rhyzopertha dominica F., after exposure to five diatomaceous earth (DE) formulations: Protect-It(R),Insecto(R), Perma-GuardTM, Dryacide(R) and SilicoSec(R). Adults of these species were exposed to DEs at the rate of 0.5 mg/cm2 for 1 day on filter paper inside plastic Petri dishes. After exposure,the initial mortality was counted and live individuals of the three species were held for a week in glass vials containing 50 mg wheat flour, rice and whole wheat, respectively. In the second experiment, after 1 day exposure to DEs, beetles were transferred to Petri dishes without food and held for a week to determine if the presence of food source would decrease the mortality of beetles. Experiments were carried out at 27℃ and 55% RH in the dark. The initial morality in both of the experiments reached 100% for the three species exposed to Protect-It(R) and in the case orR. dominica and O. surinamensis exposed to Dryacide(R). In contrast, low level of mortality (< 10%) was observed for T. castaneum exposed to Perma-GuardTM and Insecto(R). The mortality after the post-treatment period on food was decreased for the three species exposed to Perma-GuardTM and in the case of T. castaneum and R. dominica exposed to Insecto(R) and SilicoSec(R). Adults of O. surinamensis were the most susceptible followed by R. dominica and 100% adult mortality was obtained, whereas T. castaneum were the least susceptible beetles to DEs. Protect-It(R) and Dryacide(R) were the most efficient DE formulations and can be used effectively in a stored grain integrated pest management program.

  1. Effect of salt stressed wheat varieties on life history of tribolium castaneum (hebrst) (tenebrionidae: coleoptera)

    International Nuclear Information System (INIS)

    In the present investigation an attempt was made to determine the antibiosis of three salt stressed wheat varieties (Shafaq, Inqlab-91 and Sehar-2006) at three salinity levels (8, 12 and 16 dS m/sup -1/) to red flour beetle, Tribolium castaneum (Herbst). The grains of three varieties were ground and sieved through 80 mesh. Life history parameters, i.e., larval and pupal durations and survivals, adult emergence, fecundity and egg hatching was observed by introducing 10 pairs of pupae, from previously reared beetles, on three varieties at their respective salt levels. One control was included with grains of the plants, which were irrigated with distilled water only. Results have shown that varieties had significant difference among the treatments for larval duration, which was significantly extended in different varieties at various salt levels. Mean longest larval duration (31.67 days) was recorded in Shafaq as respectively 8 (33.00 days), 12 (33.33 days) and 16 (30.33 days) dS m/sup -1/ salt level as compared to its control (31.00 days). Fecundity and egg hatching of T. castaneum differed among the wheat varieties irrespective of salt levels. In another experiment, response of SARC-1, SARC-2, SARC-3, SARC-4, SARC-5, SARC-6, SARC-7, SARC-8, LU-26S, to life history parameters of T. castaneum showed that significant difference in the number of eggs, hatching percentage, larval and pupal survival was found. The number of eggs was significantly lower in variety SARC 1 (126.00) followed by SARC 2 (128.75), SARC 3 (132.25) while was significantly higher in SARC 5 (151.75). Egg hatching percentage was lower in SARC 6 (39.38%) and significantly higher in SARC 5 (58.42%). Larval survival was significantly less in SARC 7 (36.99%) and more in SAR (52.25%). Pupal survival was significantly lower in SARC 1 (20.54%) while higher in SARC 8 (42.11%). Based on results it may be stated that salt stressed wheat varieties have significant impact of the biology of T. castaneum. (author)

  2. Essential Oil and its Insecticidal Activity of Medicinal Aromatic Plant Vetiveria zizanioides (L. Against the Red Flour Beetle Tribolium castaneum (Herbst

    Directory of Open Access Journals (Sweden)

    S. Sujatha

    2010-07-01

    Full Text Available Essential oil of Vetiver has versatile uses particularly as an inexpensive yet effective and eco-friendly tool to compact the essential role of biopesticides as well as insecticidal potentials. Insecticidal activity of the root extract of Vetiveria zizanioides (L. in Petroleum ether, Ethyl acetate, Acetone and methanol against XSM, SMC, SRS and JTC strains of the red flour beetle, Tribolium castaneum (Herbst was studied. The spongy root mass were dried, powdered and extracted by Steam distillation apparatus with the solvents below 60ºC. Experimental extracts were applied on larvae and adult beetles in film residue methods and mortality was recorded after 24 h. In larval bioassay the highest toxicity was recorded for petroleum ether extract (LD50 = 0.051 :g/cm2 in XSM strain and the low est toxicity has been observed in Methanol extract (LD50 =11.351:g/cm2 in SMC strain. In adults petroleum ether extract accessible highest toxicity (LD50 = 58.69 :g/cm2 in JTC 12 strain and the lowest toxicity (LD50 = 204.710 :g/cm2 also been observed for acetone extract in SRS strain. LD50, 95% confidence limits and regression equations are presented.

  3. Residual efficacy of methoprene for control of Tribolium castaneum (Coleoptera: Tenebrionidae) larvae at different temperatures on varnished wood, concrete, and wheat

    Science.gov (United States)

    The residual efficacy of the juvenile hormone analogue, methoprene (Diacon II), was evaluated in bioassays using larvae of Tribolium castaneum (Herbst) exposed on varnished wood or unsealed concrete treated with a liquid formulation and held at different temperatures. When these surfaces were stored...

  4. WATER ALOE WEEVIL BAGOUS BINODULUS (HERBST, 1795) (COLEOPTERA: CURCULIONIDAE) IN SERBIA

    OpenAIRE

    Mesaroš, Gabor M.

    2015-01-01

    Bagous binodulus (Herbst, 1795)  was recorded in Serbia for the first time. Several specimens were collected in the Zasavica Special reserve near Sremska Mitrovica. The general information on this weevil species, including remarks about their distribution in Serbia, is given. Also, we provide the list of Bagous species of the Serbian fauna.

  5. Progeny production by Tribolium castaneum (Coleoptera: Tenebrionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae) on maize previously infested by Sitotroga cerealella (Lepidoptera: Gelechiidae).

    Science.gov (United States)

    Weston, P A; Rattlingourd, P L

    2000-04-01

    A laboratory experiment was conducted to quantify the effects of infestation of maize by Sitotroga cerealella (Olivier) on progeny production by two common secondary colonizers of grain, Tribolium castaneum (Herbst) and Oryzaephilus surinamensis (L.). Adults of both secondary pest species were allowed to oviposit for 3 wk on intact kernels of 'DeKalb 689', mechanically split kernels, kernels that had been infested for 3 mo by S. cerealella, and kernels that had been infested for 6 mo. Progeny of both species reached highest numbers on 6-mo infested maize. Prior infestation for 6 mo by S. cerealella makes maize a more suitable medium for reproduction by T. castaneum and O. surinamensis, much more than can be accounted for by mere disruption of kernel integrity resulting from larval feeding. The results highlight the importance of limiting establishment by S. cerealella on maize in storage. PMID:10826210

  6. Chemical composition of four essential oils from Eupatorium spp: Biological activities toward Tribolium castaneum (Coleoptera: Tenebrionidae Composición química de cuatro aceites esenciales provenientes de Eupatorium spp. y su toxicidad para Tribolium castaneum (Coleoptera: Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Hugo G. Lancelle

    2009-12-01

    Full Text Available Toxic and repellent properties of whole essential oils from four Eupatorium (Asteraceae species (E. buniifolium Hook. et Arn, E. inulaefolium Kunth, E. arnottii Baker, and E. viscidum Hook. & Arn were investigated in different concentrations toward Tribolium castaneum Herbst adults. The essential oils were isolated by hydrodistillation techniques from the aerial parts. The analysis was performed by GC-FID and GC-MS methods. Contact toxicity assays showed that all the evaluated essential oils were toxic. Furthermore, in all the cases mortality was dose dependent. The main repellency was observed for the essential oil recovered from E. buniifolium.Se evaluaron las propiedades tóxicas y repelentes de los aceites esenciales de cuatro especies del género Eupatorium (Asteraceae: E. buniifolium Hook. et Arn, E. inulaefolium Kunth, E. arnottii Baker y E. viscidum Hook. & Arn, en diferentes concentraciones frente a adultos de Tribolium castaneum Herbst. Los aceites esenciales se aislaron de las partes aéreas de las plantas, mediante técnicas de hidrodestilación y se analizaron por los métodos GC-FID y GC-MS. Los ensayos de toxicidad por contacto demostraron que todos los aceites fueron tóxicos y la mortalidad fue, en todos los casos, dependiente de la dosis. El aceite esencial de E. buniifolium presentó la mayor actividad repelente.

  7. Ojo blanco (obl : un gene marcador del grupo de ligamiento IV en Tribolium castaneum Herbst Ojo blanco (obl: un gene marcador del grupo de ligamiento IV en Tribolium castaneum Herbst

    Directory of Open Access Journals (Sweden)

    Nuñez Fernando

    1988-06-01

    Full Text Available

    Some genetic aspects of a spontaneous mutation in T. castaneum, discovered in the Department of Biology of National University of Colombia (Bogota, were studied. The mutant gene christened "ojo blanco" (obl, is recessive autosomic, of excellent viability, complete penetrance and variable expression. The gene is phenotipically characterized by a depigmentation of the compound eyes and can be observed from the larval stage. Linkage tests with marker mutants for different groups were carried out. The marker "sooty", of the linkage group IV, gave positive results. The distance was estimated from 2843 descendants obtained from backcrosses in repulsion plase and 1630 in coupling phase. This distance was worked out as 15.64 ± 0.92 units between obl ands. It was not possible to determine if obl is on the left or the right hand side of s. Nor discrepancies between sexes, neither between phases were found in the recombination frequencies for the region between the two loci of chromosome IV.

    Se estudiaron algunos aspectos genéticos de una mutación espontanea en T. casteneum, descubierta en el Departamento de Biología de la Universidad Nacional de Colombia (Bogotá. EI gene mutante llamado "ojo blanco" (obl es autosómico recesivo, de excelente viabilidad, penetrancia completa y expresividad variable. Fenotípicamente se caracteriza por una despigmentación de los ojos compuestos; puede observarse desde el estado de larva. Se le hicieron pruebas de ligamiento con marcadores para diferentes grupos. Se obtuvieron resultados positivos con el marcador "sooty" del grupo de ligamiento IV. La  distancia se calcu1ó a partir de 2.843 descendientes de retrocruzas en posición de repulsión y 1.630 descendientes de retrocruzas en posición de acoplamiento. Esta distancia fue de 15.ó4 ± 0.92 unidades entre obl ys. No se pudo determinar si obl esta a la izquierda 0 a la

  8. Toxic and Repellent effecto of Harmal (Peganum harmala L. Acetonic Extract on Several Aphids and Tribolium castaneum (Herbst Efecto Tóxico y Repelente del Extracto Acetónico de Harmal (Peganum harmala L. sobre varias especies de Áfidos y Tribolium castaneum (Herbst

    Directory of Open Access Journals (Sweden)

    Elham Salari

    2012-03-01

    Full Text Available To reduce the dependence on the sometimes unwise use of synthetic pesticides in fruit and vegetable plantations, the toxicity and repellence of Peganum harmala L. (Zygophyllaceae acetonic seed extract was assayed against several insect pests. For contact toxicity, 3- to 4-d-old individuals of Aphis fabae Scopoli, A. gossypii Glover, A. nerii Boyer de Fonscolombe, and Myzus persicae (Sulzer were included, as well as 1- to 7-d-old adult Tribolium castaneum (Herbst. Repellent effect experiments were conducted on adult, 1- to 2- and 3- to 4-d old M.persicae individuals. At 60 mg mL4, the topical bioassay mortality percentage was significantly higher in A. gossypii than in A. fabae and A. nerii after 12-72 h. Mortality of the treatments on M.persicae was 87.1% and 90.0% after 24 and 48 h, respectively, and significantly higher than A. fabae and A. nerii during this period. At 60 mg mL-1, the mortality of T. castaneum was much lower than that of the aphid species. The highest repellent index (over 72% was observed on 1- to 2-d-old M. persicae individuals.Para reducir la dependencia de los pesticidas sintéticos en plantaciones frutales y hortalizas, se realizó un ensayo para medir la toxicidad y repelencia de un extracto acetónico obtenido a partir de semillas de Peganum harmala L. (Zygophyllaceae contra diferentes especies de plagas. Para evaluar la toxicidad del extracto al contacto con los insectos, se incluyeron individuos de 3-4 d de edad de Aphis fabae Scopoli, Aphis gossypii Glover, Aphis nerii Boyer de Fonscolombe, y Myzus persicae (Sulzer, así como adultos 1-7 d de edad de Tribolium castaneum (Herbst. Experimentos para medir el efecto repelente se llevaron a cabo con individuos de 1-2 y 3-4 d de edad de M. persicae. En los resultados de los bioensayos tópicos el porcentaje de mortalidad fue significativamente mayor en la especie A. gossypii que en A. fabae y A. nerii, después de 12-72 h con una concentración de 60 mg mL-1. La mortalidad

  9. The Presence of Flour Affects the Efficacy of Aerosolized Insecticides used to Treat the Red Flour Beetle, Tribolium castaneum

    OpenAIRE

    Toews, Michael D; Campbell, James F.; Arthur, Franklin H.

    2010-01-01

    Experiments were conducted in tightly sealed pilot scale warehouses to assess the efficacy of common aerosolized insecticides on all life stages of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) when exposed in dishes containing 0 to 2 g of wheat flour either under pallets or out in the open. Petri dishes containing 0, 0.1, 1, or 2 g of flour were prepared with 25 eggs, 3rd instars, pupae, or adults and then immediately treated with aerosolized solvent, Pyrethrins, or esfenvalerate....

  10. The complete mitochondrial genome of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Liu, Qiu-Ning; Bian, Dan-Dan; Jiang, Sen-Hao; Li, Zhen-Xing; Ge, Bao-Ming; Xuan, Fu-Jun; Yang, Li; Li, Chao-Feng; Zhang, Dai-Zhen; Zhou, Chun-Lin; Tang, Bo-Ping

    2016-01-01

    The complete mitochondrial genome (mitogenome) of Tribolium castaneum (Coleoptera: Tenebrionidae) was determined to be 15,883 bp (GenBank accession No. KM009121), which contains 22 tRNA genes, 13 protein-coding genes (PCGs), 2 rRNA genes and a major non-coding A + T-rich region. It has the typical gene organization and order of mitogenomes from ancestral insects. The nucleotide composition was also biased toward A + T nucleotides (71.72%) and the AT skew of this mitogenome was slightly positive. All of the 22 tRNA genes displayed a typical clover-leaf structure, with the exception of trnS1 (AGN). Thirteen PCGs were initiated by ATN codons, except for cytochrome c oxidase subunit 1 (cox1) gene which was initiated by AAT. Eight of the 13 PCGs harbor the incomplete termination codon by T or TA. The A + T-rich region of the mitogenome was 1237 bp in length and the A + T content was 82.30%. PMID:25162515

  11. LONG-TERM EFFICACY OF PROTEIN-ENRICHED PEA FLOUR AGAINST TRIBOLIUM CASTANEUM (COLEOPTERA: TENEBRIONIDAE IN WHEAT FLOUR

    Directory of Open Access Journals (Sweden)

    P Pretheep-Kumar

    2007-07-01

    Full Text Available Long-term efficacy of the protein-enriched flour of pea (Pisum sativum L. var. Bonneville in its toxicity, progeny reduction and organoleptic properties was evaluated by combining it with wheat flour and testing the admixture against the red flour beetle, Tribolium castaneum (Herbst. The toxicity and progeny-reducing effects of the wheat flour treated with protein-enriched pea flour were stable for a period of 5 months when stored at 28°C with 75% r.h. Heat treatment destroyed the biological activity of the protein-enriched pea flour containing the active ingredient due to the denaturation of proteins. The organoleptic properties of stored wheat flour were not affected by the treatment with protein-enriched pea flour.

  12. Effect of Gamma Radiation on Nutritional Indices of Larval and Adults Stages of Tribolium castaneum (Coleoptera: Tenebrionidae)

    International Nuclear Information System (INIS)

    In this study antifeedant effect of different doses of gamma radiation as a controlling safe method on flour weevil, Tribolium castaneum (Herbst) larvae and adult was studied. Doses of 100, 400, 600, 800 and 1000 Gy of gamma radiation were used and after 72 hours, nutritional indices were evaluated. The relative growth rate, relative consumption rate, efficiency of conversion of ingested food and feeding deterrence index as nutritional indices were evaluated. Treatments were assessed by flour wheat disc at 27±1degreeC and 65percenthumidity in a dark condition. The results showed that the relative growth rate of flour weevil larvae and adults decreased significantly (P<0.05) by gamma radiation and the severity of this reduction in larvae was higher than the adults. Although the relative growth rates decreased in adults, this rate in doses of 400, 600, 800 and 1000 Gy showed no significant difference. The relative food consumption rate also decreased with the gamma radiation and its value found to be inversely proportional to the dose radiation. Our experiments showed that the use of gamma radiation exposure to 800 Gy had no significant effect on the efficiency of conversion of ingested food of larvae and reduction was observed only when the gamma radiation was used in 1000 Gy. The feeding deterrence effect of gamma radiation, especially on the larvae was high but no significant difference between doses of 100 to 800 Gy was observed. The results showed that gamma radiation that induces antifeedant effect can be applied as an effective method in control of T. castaneum.

  13. Elucidating the Common Generalist Predators of Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae) in an Organic Apple Orchard Using Molecular Gut-Content Analysis.

    Science.gov (United States)

    Schmidt, Jason M; Szendrei, Zsofia; Grieshop, Matthew

    2016-01-01

    Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), plum curculio, is a serious direct pest of North American tree fruit including, apples, cherries, peaches and plums. Historically, organophosphate insecticides were used for control, but this tool is no longer registered for use in tree fruit. In addition, few organically approved insecticides are available for organic pest control and none have proven efficacy as this time. Therefore, promoting biological control in these systems is the next step, however, little is known about the biological control pathways in this system and how these are influenced by current mechanical and cultural practices required in organic systems. We used molecular gut-content analysis for testing field caught predators for feeding on plum curculio. During the study we monitored populations of plum curculio and the predator community in a production organic apple orchard. Predator populations varied over the season and contained a diverse assemblage of spiders and beetles. A total of 8% of all predators (eight Araneae, two Hemiptera, and six Coleoptera species) assayed for plum curculio predation were observed positive for the presence of plum curculio DNA in their guts, indicating that these species fed on plum curculio prior to collection Results indicate a number of biological control agents exist for this pest and this requires further study in relation to cultural practices. PMID:27348005

  14. Biological effects of gamma radiation on stored product insects. 4 - radiation effects on sex pheromone production and perception by the rust-red flour beetle. Tribolium castaneum (herbst)

    OpenAIRE

    Abdu, R. M.; Abdel-Kader, Maissa M.; M. A. Hussein; Abdel-Rahman, H. A.

    1985-01-01

    Irradiation of the rust-red flour beetle, T. castaneum at different doses of gamma radiation considerably affected sex pheromone production by females and perception by males. The production of sex pheromone by virgin females decreased with the increase of radiation doses from 4 to 10 krad., and a dose of 12 krad could almost inhibit pheromone production. Males were more radiosensitive in their response to sex pheromone; and a radiation dose of 8 krad could brought inhibition of male respo...

  15. Concentration dependent toxicokinetics of copper in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Bednarska, Agnieszka J; Stępień, Katarzyna

    2015-11-01

    To predict internal metal concentrations in animals under specific environmental exposures, the relationship between the exposure concentrations and values of toxicokinetic parameters must be known. At high exposure levels, the availability of carriers transporting metal ions through cellular membranes may become limited, thereby decreasing the assimilation rates (k A ). Furthermore, increased metal concentrations in food may result in greater damage to the gut and reduce the assimilation efficiency and/or increase the elimination rate (k E ). Therefore, k A should decrease and k E should increase with increasing metal concentrations. In fact, our study on Tribolium castaneum exposed to Cu at 500, 1000, 2000 and 4000 mg kg(-1) of dry flour showed that with increasing Cu concentrations, k A decreased from 0.0042 day(-1) at 500 mg kg(-1) to 0.0026 day(-1) at 4000 mg kg(-1) in females and from 0.0029 to 0.001 day(-1) in males and k E increased from 0.027 to 0.064 day(-1) and from 0.018 to 0.04 day(-1) in females and males, respectively. Significant differences in k A between the sexes were observed at 2000 and 4000 mg kg(-1), whereas significant differences between treatments were found for k A in males. Copper was efficiently regulated by T. castaneum: an eightfold increase in exposure concentrations resulted in only a ca. twofold increase in the internal concentration. No Cu effect on the respiratory metabolism of T. castaneum was found. PMID:26169625

  16. Lethal dose determination of Cobalt-60 for adult Tribolium castaneum (Coleoptera: Tenebrionidae) and Cryptolestes ferrugineus (Coleoptera: Laemophloeidae)

    Energy Technology Data Exchange (ETDEWEB)

    Farias, Anderson Aparecido, E-mail: potenza@biologico.sp.gov.br [Instituto Biologico, Centro de Pesquisa e Desenvolvimento de Protecao Ambiental, Sao Paulo, SP (Brazil); Potenza, Marcos Roberto, E-mail: fcreis@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Reis, Fabricio Caldeira; Sato, Mario Eidi, E-mail: mesato@biologico.sp.gov.br [Instituto Biologico, Centro Experimental Central, Laboratorio de Acarologia, Campinas, SP (Brazil)

    2015-07-01

    The insect infestation is a major problem in the grain storage. The pesticides are most widely used method for disinfestation and prevention. Treatment with gamma irradiation may increase the product shelf life without encountering formation of waste can be used in packaged foods and ready for commercialization, representing an important alternative to the use of pesticides. This study aimed to determine the immediate lethal dose of gamma radiation for adults of Tribolium castaneum and Cryptolestes ferrugineus. The study was conducted in the Instituto Biologico and the radiations held at the Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN, Sao Paulo city, using a multipurpose irradiator Cobalt-60 with 3.31 dose rate and 3.23 kGy in months of November/2014 and January/2015. Each experimental unit consisted of 20 adult insects, confined in a 10 mL polyethylene container. The experimental plots in number 10 per dose were subjected to increasing doses of gamma radiation: 0; 0.5; 0.75; 1.0; 1.25; 1.50; 1.75; 2.00; 2:50 and 3.00 kGy. Mortality was assessed within two to four hours after irradiation. The data were submitted to Probit analysis, using the POLO PLUS program. The LD90 to control 90% (LD90) to control adult Cryptolestes ferrugineus and Tribolium castaneum were 2.73 and 2.91 kGy, respectively. (author)

  17. Lethal dose determination of Cobalt-60 for adult Tribolium castaneum (Coleoptera: Tenebrionidae) and Cryptolestes ferrugineus (Coleoptera: Laemophloeidae)

    International Nuclear Information System (INIS)

    The insect infestation is a major problem in the grain storage. The pesticides are most widely used method for disinfestation and prevention. Treatment with gamma irradiation may increase the product shelf life without encountering formation of waste can be used in packaged foods and ready for commercialization, representing an important alternative to the use of pesticides. This study aimed to determine the immediate lethal dose of gamma radiation for adults of Tribolium castaneum and Cryptolestes ferrugineus. The study was conducted in the Instituto Biologico and the radiations held at the Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN, Sao Paulo city, using a multipurpose irradiator Cobalt-60 with 3.31 dose rate and 3.23 kGy in months of November/2014 and January/2015. Each experimental unit consisted of 20 adult insects, confined in a 10 mL polyethylene container. The experimental plots in number 10 per dose were subjected to increasing doses of gamma radiation: 0; 0.5; 0.75; 1.0; 1.25; 1.50; 1.75; 2.00; 2:50 and 3.00 kGy. Mortality was assessed within two to four hours after irradiation. The data were submitted to Probit analysis, using the POLO PLUS program. The LD90 to control 90% (LD90) to control adult Cryptolestes ferrugineus and Tribolium castaneum were 2.73 and 2.91 kGy, respectively. (author)

  18. Effect of combination of gamma radiation and essential oil from perovskia atriplicifolia on mortality of tribolium castaneum

    International Nuclear Information System (INIS)

    In an attempt to find a natural and inexpensive method to control the stored-product pests, the effect of combination of gamma radiation and essential oil from Perovskia atriplicifolia (Benth) on the adults of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) was investigated. Experiments were carried out at 27±ldegC and 65±5% R. H. under dark condition. Two experimental regimes were designed: 1) irradiation of fumigated adults 2)fumigation of irradiated adults. In combination experiments, mortality was assessed 14 days after the first treatment. The results showed significant synergistic effect of gamma radiation with essential oil on adults of T. castaneum (1-7 days old). Gamma irradiation at dosage 100 Gy, alone caused 12.5% mortality on T. castaneum but when these irradiated insects were treated by 7.66 μ1/1 air of P. atriplicifolia oil 7 days after irradiation, (caused 6.25% mortality alone) mortality percent reached 32.5%. These results provide the basis for successful use of gamma radiation in the presence of the essential oil for management of T. castaneum.

  19. The SNMP/CD36 gene family in Diptera, Hymenoptera and Coleoptera: Drosophila melanogaster, D. pseudoobscura, Anopheles gambiae, Aedes aegypti, Apis mellifera, and Tribolium castaneum.

    Science.gov (United States)

    Nichols, Zachary; Vogt, Richard G

    2008-04-01

    Sensory neuron membrane proteins (SNMPs) are membrane bound proteins initially identified in olfactory receptor neurons of Lepidoptera and are thought to play a role in odor detection; SNMPs belong to a larger gene family characterized by the human protein CD36. We have identified 12-14 candidate SNMP/CD36 homologs from each of the genomes of Drosophila melanogaster, D. pseudoobscura, Anopheles gambiae and Aedes aegypti (Diptera), eight candidate homologs from Apis mellifera (Hymenoptera), and 15 from Tribolium castaneum (Coleoptera). Analysis (sequence similarity and intron locations) suggests that the insect SNMP/CD36 genes fall into three major groups. Group 1 includes the previously characterized D. melanogaster emp (epithelial membrane protein). Group 2 includes the previously characterized D. melanogaster croquemort, ninaD, santa maria, and peste. Group 3 genes include the SNMPs, which fall into two subgroups referred to as SNMP1 and SNMP2. D. melanogaster SNMP1 (CG7000) shares both significant sequence similarity and five of its six intron insertion sites with the lepidopteran Bombyx mori SNMP1. The topological conservation of this gene family within the three major holometabolous lineages indicates that it predates the coleopteran and hymenoptera/dipera/lepidoptera split 300+ million years ago. The current state of knowledge of the characterized insect members of this gene family is discussed. PMID:18342246

  20. Chaves para a identificação dos principais Coleoptera (Insecta associados com produtos armazenados Keys for the identification of Coleoptera (Insecta associated with stored products

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Valle da Silva Pereira

    2001-03-01

    Full Text Available An illustrated key to identify nine families of Coleoptera commonly found in stored products is presented. Keys for the identification of Anobiidae [Lasioderma serricorne (Fabricius, 1792, Stegobium paniceum (Linnaeus, 1761], Bruchidae [Acanthoscelides obtectus (Say, 1831, Zabrotes subfasciatus (Boheman, 1833], Curculionidae [Sitophilus oryzae (Linnaeus, 1763, S. zeamais Motschulsky, 1885], Silvanidae [Ahasverus advena (Waltl, 1832, Cathartus quadricollis (Guérin, 1892, Oryzaephilus mercator (Fauvel, 1889, O. surinamensis (Linnaeus, 1758] and Tenebrionidae [Gnathocerus cornutus (Fabricius, 1798, Tribolium castaneum (Herbst, 1797, T. confusum du Val, 1868] are also provided. These keys cover the most frequent Coleoptera found in stored products, specially grains, and are to the adult stage only. Illustrations of external morphology and general characteristics are provided for each species reported.

  1. Influência da temperatura da massa de grãos sobre a toxicidade do ozônio a Tribolium castaneum Influence of the grain temperature on the ozone toxicity to Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Alexandre de M. Pereira

    2008-10-01

    Full Text Available Considerando que a temperatura da massa de grãos pode influenciar o período de exposição dos fumigantes, desenvolveu-se este trabalho com o propósito de investigar a influência de temperaturas crescentes da massa de grãos (20, 30, 35 e 40 ºC no período de exposição do ozônio necessário para matar 50 e 95% de insetos adultos de Tribolium castaneum Herbst (Coleoptera: Tenebrionidae. Grãos de milho foram distribuídos em recipientes cilíndricos de PVC com conexões para injeção e exaustão de gás. Os insetos contidos em gaiolas foram colocados na camada mediana da massa de grãos em cada recipiente e estes, por sua vez foram fumigados com ozônio na concentração de 50 ppm. Os tempos letais (TL50 e TL95 para os insetos adultos de T. castaneum foram reduzidos com o aumento da temperatura da massa de grãos, de 20 para 30 ºC, porém a 35 ºC os tempos letais não foram reduzidos em relação às temperaturas menores, mas a 40 ºC os tempos letais foram reduzidos novamente, resultando no menor TL95.Considering that the grain temperature may influence the exposure time of the fumigants, the present study was carried out to investigate the influence of increasing temperatures of the grain mass (20, 30, 35 and 40 ºC on the exposure time to ozone required to kill 50 and 95% of adult insects of Tribolium castaneum (Herbst (Coleoptera: Tenebrionidae. Maize grains were distributed in cylindrical polyvinyl chloride recipients having connections for gas injection and exhaustion. The insects held in cages were placed at the median height of the grain mass in each recipient, which was then fumigated with ozone at the concentration of 50 ppm. The lethal times (LT50 and LT95 for adult insects of T. castaneum were reduced by increasing grain temperature, from 20 to 30 ºC; however, at 35 ºC the lethal times were not reduced compared with lower temperatures; but at 40 ºC the exposure time decreased again, resulting in lower LT95.

  2. Toxicidade da combinação de dióxido de carbono e fosfina sob diferentes temperaturas para Tribolium castaneum Toxicity of the carbon dioxide and phosphine combination to Tribolium castaneum under different temperatures

    Directory of Open Access Journals (Sweden)

    Raimundo W. S. Aguiar

    2010-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da temperatura sobre a toxicidade da combinação de dióxido de carbono e fosfina, para os estágios de desenvolvimento de Tribolium castaneum (Herbst (Coleoptera: Tenebrionidae. A toxicidade da combinação de 5% de dióxido de carbono e 1 g m-3 de fosfina para os estágios de ovo, larvas de 5, 10 e 15 dias, pupa e adulto de T. castaneum, foi estudada nas temperaturas de 25, 30, 35, 40 e 45 °C, por meio de estimativas dos tempos de exposição letais para 50 e 95% dos insetos (TL50 e TL95. Curvas tempo-resposta foram estabelecidas mediante bioensaios com períodos crescentes de exposição à combinação do dióxido de carbono com a fosfina. Observou-se que os TL50 e TL95 reduziram com a elevação da temperatura em todos os estágios de T. castaneum avaliados. O estágio de larva de cinco dias foi a mais susceptível à combinação de dióxido de carbono e fosfina. De acordo com os resultados, a combinação do dióxido de carbono com a fosfina é alternativa potencial para diminuir a quantidade de fosfina aplicada em produtos armazenados, por apresentar alta toxicidade para todos os estágios de T. castaneum sob diferentes temperaturas.The objective of this work was to assess the effect of temperature on the toxicity of the carbon dioxide-phosphine combination for the developmental stages of Tribolium castaneum (Herbst (Coleoptera: Tenebrionidae. The toxicity of combination of 5% carbon dioxide and 1 g m-3 phosphine in the developmental stages of egg, larvae of 5, 10 and 15 days, pupae and adult of T. castaneum was studied under the temperatures of 25, 30, 35, 40 and 45 °C, through the estimation of lethal insect exposure times of 50 and 95% (LT50 and LT95. For that, time-response curves were established through bioassays with increasing periods of exposure to the combination of carbon dioxide and phosphine. A reduction of LT50 and LT95 was observed with temperature increase in all

  3. Insecticide Activity of Essential Oils of Mentha longifolia, Pulicaria gnaphalodes and Achillea wilhelmsii Against Two Stored Product Pests, the Flour Beetle, Tribolium castaneum, and the Cowpea Weevil, Callosobruchus maculatus

    Science.gov (United States)

    Khani, Abbas; Asghari, Javad

    2012-01-01

    Essential oils extracted from the foliage of Mentha longifolia (L.) (Lamiales: Lamiaceae) and Pulicaria gnaphalodes Ventenat (Asterales: Asteraceae), and flowers of Achillea wilhelmsii C. Koch (Asterales: Asteraceae) were tested in the laboratory for volatile toxicity against two storedproduct insects, the flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) and the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Bruchidae). The chemical composition of the isolated oils was examined by gas chromatography-mass spectrometry. InM longifolia, the major compounds were piperitenon (43.9%), tripal (14.3%), oxathiane (9.3%), piperiton oxide (5.9%), and d-limonene (4.3%). In P. gnaphalodes, the major compounds were chrysanthenyl acetate (22.38%), 2L -4L-dihydroxy eicosane (18.5%), verbenol (16.59%), dehydroaromadendrene (12.54%), β-pinen (6.43%), and 1,8 cineol (5.6%). In A. wilhelmsii, the major compounds were 1,8 cineole (13.03%), caranol (8.26%), alpha pinene (6%), farnesyl acetate (6%), and p-cymene (6%). C maculatus was more susceptible to the tested plant products than T castaneum. The oils of the three plants displayed the same insecticidal activity against C. maculatus based on LC50 values (between 1.54µl/L air in P. gnaphalodes, and 2.65 µl/L air in A. wilhelmsii). While the oils of A. wilhelmsii and M. longifolia showed the same strong insecticidal activity against T. castaneum (LC50 = 10.02 and 13.05 µl/L air, respectively), the oil of P. gnaphalodes revealed poor activity against the insect (LC50 = 297.9 µl/L air). These results suggested that essential oils from the tested plants could be used as potential control agents for stored-product insects. PMID:23413994

  4. Efficacy of aerosol applications of methoprene and synergized pyrethrin against Tribolium castaneum adults and eggs

    Science.gov (United States)

    Experiments were performed to determine the efficacy of a single aerosol application of the insecticides methoprene and piperonyl butoxide-synergized pyrethrin, alone or in combination, and the insecticide carrier, Isopar M, against Tribolium castaneum (Herbst), the red flour beetle. The initial tes...

  5. The presence of flour affects the efficacy of aerosolized insecticides used to treat the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Toews, Michael D; Campbell, James F; Arthur, Franklin H

    2010-01-01

    Experiments were conducted in tightly sealed pilot scale warehouses to assess the efficacy of common aerosolized insecticides on all life stages of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) when exposed in dishes containing 0 to 2 g of wheat flour either under pallets or out in the open. Petri dishes containing 0, 0.1, 1, or 2 g of flour were prepared with 25 eggs, 3rd instars, pupae, or adults and then immediately treated with aerosolized solvent, Pyrethrins, or esfenvalerate. Twenty-four h after insecticide exposure, the dishes were brought to the laboratory and placed in a growth chamber and held for a 3 day moribund (knockdown) assessment and a 21 day mortality assessment. Mortality in untreated controls was generally less than 10%, with the exception of the 21 day counts of adults and eggs. Solvent-treated replications followed similar trends, except that additional mortality was observed in exposed larvae and pupae. In the insecticide-treated dishes, mortality of T. castaneum provisioned with flour generally showed a linear decrease with increasing flour deposits. Regardless of life stage, mortality did not exceed 60% when individuals were exposed in petri dishes containing 2 g of flour. Exposure location also made a significant difference in observed mortality. While mortality never exceeded 75% in dishes positioned under pallets, there was never less than 80% mortality in dishes exposed in the open. Although there was a perceptible increase in mortality with esfenvalerate compared to Pyrethrins, these differences were considerably less than the variation observed among flour deposits. The study suggests that sanitation and preparation prior to aerosol insecticide treatments were more important than choice of a particular insecticide. PMID:21268701

  6. Food source provisioning and susceptibility of immature and adult Tribolium castaneum on concrete partially treated with chlorfenapyr (Phantom®)

    Science.gov (United States)

    A series of experiments were conducted in which adults, pupae, and 4-week-old larvae of Tribolium castaneum (Herbst), the red flour beetle, were exposed separately on concrete arenas partially treated (14.4 % of the total area) with the insecticide chlorfenapyr (Phantom®) at 1.1 g active ingredient/...

  7. Residual efficacy of aerosols to control Tribolium castaneum and Tribolium confusum

    OpenAIRE

    Arthur, F. H

    2010-01-01

    Aerosol insecticides can be important components of insect management plans for mills, food warehouses, and processing plants. In the United States, synergized pyrethrin is used alone or combined with an insect growth regulator (IGR), either methoprene or hydroprene. The presence of food material can result in increased survival of adult Tribolium castaneum (Herbst) or Tribolium confusum Jacquelin du Val exposed to synergized pyrethrins, but larvae appear to be more susceptible than adults. R...

  8. Herbst treatment in late adolescence

    DEFF Research Database (Denmark)

    Bakke, Merete; Paulsen, H U

    1989-01-01

    during retention necessitated retreatment. Treatment resulted in marked dentoalveolar changes, increase in mandibular length and ramus height, apposition and remodelling of condylar heads and flattening of the temporal joint surface. After treatment masseter strength was 40 per cent reduced, probably due......In an almost full-grown male of 17 years, functional and morphological changes were followed during Herbst treatment and subsequent control. Retrognathism of the mandible was overcorrected, and then normalized by reverse headgear to the maxilla. Partial relapse due to insufficient cooperation...

  9. Influence of age, mating status, sex, quantity of food, and long-term food deprivation on red flour beetle (Coleoptera: Tenebrionidae) flight initiation

    Science.gov (United States)

    Effects of age, sex, presence or absence of food, mating status, quantity of food, and food deprivation on rate of and time of flight initiation of the red flour beetle, Tribolium castaneum (Herbst), were determined. Flight initiation declined with increasing age in both presence and absence of food...

  10. Behavioral responses of plum curculio (Coleoptera: Curculionidae) to different enantiomer concentrations and blends of the synthetic aggregation pheromone grandisoic acid

    Science.gov (United States)

    Host plant odors are important for insect location of food and mates. Synergy between host plant odors and aggregation pheromones occurs in many Curculionidae species. The plum curculio Conotrachelus nenuphar Herbst (Coleoptera: Curculionidae) is a major pest of pome and stone fruit. Males produce t...

  11. Combination of gamma radiation and essential oils from medicinal plants in managing Tribolium castaneum contamination of stored products

    International Nuclear Information System (INIS)

    Effectiveness of management of insect infestation of stored products with essential oils as viable alternatives to synthetic insecticides can be enhanced with gamma radiation. We studied effects of sublethal doses of essential oils from Rosmarinus officinalis (L.) and Perovskia atriplicifolia (Benth) (safe natural insecticides) in combination with gamma radiation on mortality of adults of Tribolium castaneum (Herbst). The insects were subjected to two radiation doses and two concentrations of the essential oils in the air. This combined treatment increased the mortality, which was also 3–6 times higher than could be expected from the sum of the effects of each of the treatments. The synergistic effect was more pronounced in the case of R. officinalis (L.) than in the case of P. atriplicifolia (Benth). The experiments have shown that the known insecticidal effectiveness of the essential oils can be enhanced by preliminary irradiation. Possible approaches to implementation of the combined treatment are discussed. - Highlights: • The mortality of T. castaneum increased with an increase of the radiation dose. • R. officinalis was more toxic to T. castaneum than P. atriplicifolia. • Gamma radiation and essential oils could be used as combined methods in IPM. • Combination of radiation with essential oils made a synergistic effect. • The synergistic effect of the R–G was much more appropriate from P–G

  12. Efficacy of aerosol applications of methoprene and synergized pyrethrin against Tribolium castaneum adults and eggs.

    Science.gov (United States)

    Tucker, Angela M; Campbell, James F; Arthur, Frank H; Zhu, Kun Yan

    2014-06-01

    Experiments were performed to determine the efficacy of a single aerosol application of the insecticides methoprene and piperonyl butoxide-synergized pyrethrin, alone or in combination, and the insecticide carrier, Isopar M, against Tribolium castaneum (Herbst), the red flour beetle. The initial test exposed adults to insecticide treatments and placed male/female pairs in flour. All adults exposed to synergized pyrethrin were knocked down for at least 24 h after exposure but they recovered. High adult survival and similar average numbers of living F1 progeny were produced regardless of treatment exposure. In a separate test, insecticide treatments were directly applied to newly laid eggs, which resulted in the suppression of egg hatch. Synergized pyrethrin was the most effective insecticide (P < or = 0.001) for suppressing egg hatch. The effect of flour on insecticide activity to eggs and consequent insect development was also evaluated. An amount of 0.01 g of flour in the exposure arena, 62-cm2 area, was not sufficient for individuals to develop beyond the early larval stages, regardless of the treatment. As the flour amount in the arena increased from 1 to 5 g, the number of eggs that could develop to the adult stage increased, but this number was significantly lower in the insecticide treatments than in the control or carrier treatments. The results of the later tests indicate a high efficacy of the insecticides alone or in combination on T. castaneum egg hatch and development to the adult stage. PMID:25026694

  13. Comparative toxicity and micronuclei formation in Tribolium castaneum, Callosobruchus maculatus and Sitophilus oryzae exposed to high doses of gamma radiation

    International Nuclear Information System (INIS)

    The effects of gamma radiation on mortality and micronucleus formation in Tribolium castaneum Herbst, Callosobruchus maculatus (F.) and Sitophilus oryzae (L.) genital cells were evaluated. Two groups of healthy and active adult insects 1–3 and 8–10 days old were irradiated with various doses (50–200 Gy) gamma ray. Seven days post-irradiation; mortality rates and micronucleus formation were assessed in genital cells of the irradiated insects. The results show that with increasing gamma doses, the mortality rate of each species increased and T. castaneum and S. oryzae showed the low and high sensitivity respectively. It was shown that the micronucleus appearance in the tested insects had correlation with amount and intensity of radiation doses. Moreover our results indicate different levels in the genotoxicity of gamma radiation among the insects' genital cells under study. The frequency of micronuclei in genital cells of 1–3 days old insects exposed to 50 and 200 Gy were 12.6 and 38.8 Mn/1000 cells in T. castaneum, 20.8 and 46.8 Mn/1000 cells in C. maculatus and 16.8 and 57.2 Mn/1000 cells in S. oryzae respectively. A high sensitivity of the genital cells to irradiation exposure was seen in S. oryzae correlated with its high mortality rate compared with the other two species. These results might be indicative of inflicting chromosomal damage expressed as micronucleus in high mortality rates observed in the pest population; an indication of genotoxic effects of radiation on the studied species. - Highlights: • C. maculatus was more susceptible than that of the T. castaneum to irradiation. • S. oryzae was more susceptible to irradiation than of those other two species. • The incidence of Mn is correlated to the loss of chromosome segments. • Gamma radiation has the ability to create micronuclei in insects. • Mn induced in the genital cells of adult increased with increasing radiation doses

  14. Combination of gamma radiation and essential oils from medicinal plants in managing Tribolium castaneum contamination of stored products.

    Science.gov (United States)

    Ahmadi, Mehrdad; Abd-alla, Adly Mohamed M; Moharramipour, Saeid

    2013-08-01

    Effectiveness of management of insect infestation of stored products with essential oils as viable alternatives to synthetic insecticides can be enhanced with gamma radiation. We studied effects of sublethal doses of essential oils from Rosmarinus officinalis (L.) and Perovskia atriplicifolia (Benth) (safe natural insecticides) in combination with gamma radiation on mortality of adults of Tribolium castaneum (Herbst). The insects were subjected to two radiation doses and two concentrations of the essential oils in the air. This combined treatment increased the mortality, which was also 3-6 times higher than could be expected from the sum of the effects of each of the treatments. The synergistic effect was more pronounced in the case of R. officinalis (L.) than in the case of P. atriplicifolia (Benth). The experiments have shown that the known insecticidal effectiveness of the essential oils can be enhanced by preliminary irradiation. Possible approaches to implementation of the combined treatment are discussed. PMID:23632647

  15. Activities of modified Cry3A-type toxins on the red flour beetle, Tribolium castaneum (Herbst)

    Czech Academy of Sciences Publication Activity Database

    Mostafa, Moataz; Vlasák, Josef; Sehnal, František

    2013-01-01

    Roč. 137, č. 9 (2013), s. 684-692. ISSN 0931-2048 R&D Projects: GA MZe QI91A229; GA MZe QH71290 Institutional support: RVO:60077344 Keywords : Bacillus thuringensis * biocontrol * Cry3Aa Subject RIV: ED - Physiology Impact factor: 1.701, year: 2013 http://onlinelibrary.wiley.com/doi/10.1111/jen.12054/pdf

  16. Chemical constituents and biological activities against Tribolium castaneum (Herbst) of the essential oil from Citrus wilsonii leaves

    OpenAIRE

    Chen Hai Ping; Yang Kai; You Chun Xue; Du Shu Shan; Cai Qian; He Qing; Geng Zhu Feng; Deng Zhi Wei

    2014-01-01

    The essential oil obtained from Citrus wilsonii Tanaka leaves with hydrodistillation was investigated by GC and GC-MS. The main components of the essential oil were identified to be citronellol (16.94%), nerol acetate (10.35%), γ-terpinen (9.85%), citronellal (9.36%) and β-pinene (6.72%). Among them, the four active constituents, predicted with a bioactivity-test, were isolated and identified as citronellol, γ-terpinene, nerol (neryl) acetate and β-pinene. ...

  17. Identification and evolution of two insulin receptor genes involved in Tribolium castaneum development and reproduction.

    Science.gov (United States)

    Sang, Ming; Li, Chengjun; Wu, Wei; Li, Bin

    2016-07-10

    The insulin and insulin-like signaling (IIS) pathway exists in a wide range of organisms from mammals to invertebrates and regulates several vital physiological functions. A phylogenetic analysis have indicated that insulin receptors have been duplicated at least twice among vertebrates, whereas only one duplication occurred in insects before the differentiation of Coleoptera, Hymenoptera, and Hemiptera. Thus, we cloned two putative insulin receptor genes, T.cas-ir1 and T.cas-ir2, from T. castaneum and determined that T.cas-ir1 is most strongly expressed during the late adult and early pupal stages, whereas T.cas-ir2 is most strongly expressed during the late larval stage. We found that larval RNAi against T.cas-ir1 and T.cas-ir2 causes 100% and 42.0% insect death, respectively, and that parental RNAi against T.cas-ir1 and T.cas-ir2 leads to 100% and 33.3% reductions in beetle fecundity, respectively. The hatching rate of ds-ir2 insects was 66.2%. Moreover, RNAi against these two genes increased the expression of the pkc, foxo, jnk, cdc42, ikk, and mekk genes but decreased erk gene expression. Despite these similarities, these two genes act via distinct regulatory pathways. These results indicate that these two receptors have functionally diverged with respect to the development and reproduction of T. castaneum, even though they retain some common regulatory signaling pathways. PMID:26923187

  18. Comparative toxicity and micronuclei formation in Tribolium castaneum, Callosobruchus maculatus and Sitophilus oryzae exposed to high doses of gamma radiation.

    Science.gov (United States)

    Ahmadi, Mehrdad; Mozdarani, Hossein; Abd-Alla, Adly M M

    2015-07-01

    The effects of gamma radiation on mortality and micronucleus formation in Tribolium castaneum Herbst, Callosobruchus maculatus (F.) and Sitophilus oryzae (L.) genital cells were evaluated. Two groups of healthy and active adult insects 1-3 and 8-10 days old were irradiated with various doses (50-200 Gy) gamma ray. Seven days post-irradiation; mortality rates and micronucleus formation were assessed in genital cells of the irradiated insects. The results show that with increasing gamma doses, the mortality rate of each species increased and T. castaneum and S. oryzae showed the low and high sensitivity respectively. It was shown that the micronucleus appearance in the tested insects had correlation with amount and intensity of radiation doses. Moreover our results indicate different levels in the genotoxicity of gamma radiation among the insects' genital cells under study. The frequency of micronuclei in genital cells of 1-3 days old insects exposed to 50 and 200 Gy were 12.6 and 38.8 Mn/1000 cells in T. castaneum, 20.8 and 46.8 Mn/1000 cells in C. maculatus and 16.8 and 57.2 Mn/1000 cells in S. oryzae respectively. A high sensitivity of the genital cells to irradiation exposure was seen in S. oryzae correlated with its high mortality rate compared with the other two species. These results might be indicative of inflicting chromosomal damage expressed as micronucleus in high mortality rates observed in the pest population; an indication of genotoxic effects of radiation on the studied species. PMID:25898238

  19. Larval RNA interference in the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Linz, David M; Clark-Hachtel, Courtney M; Borràs-Castells, Ferran; Tomoyasu, Yoshinori

    2014-01-01

    The red flour beetle, Tribolium castaneum, offers a repertoire of experimental tools for genetic and developmental studies, including a fully annotated genome sequence, transposon-based transgenesis, and effective RNA interference (RNAi). Among these advantages, RNAi-based gene knockdown techniques are at the core of Tribolium research. T. castaneum show a robust systemic RNAi response, making it possible to perform RNAi at any life stage by simply injecting double-stranded RNA (dsRNA) into the beetle's body cavity. In this report, we provide an overview of our larval RNAi technique in T. castaneum. The protocol includes (i) isolation of the proper stage of T. castaneum larvae for injection, (ii) preparation for the injection setting, and (iii) dsRNA injection. Larval RNAi is a simple, but powerful technique that provides us with quick access to loss-of-function phenotypes, including multiple gene knockdown phenotypes as well as a series of hypomorphic phenotypes. Since virtually all T. castaneum tissues are susceptible to extracellular dsRNA, the larval RNAi technique allows researchers to study a wide variety of tissues in diverse contexts, including the genetic basis of organismal responses to the outside environment. In addition, the simplicity of this technique stimulates more student involvement in research, making T. castaneum an ideal genetic system for use in a classroom setting. PMID:25350485

  20. Ionizing radiation control of Tribolium castaneum in wheat flour type 000

    International Nuclear Information System (INIS)

    The insects, mainly those of the coleoptera order, produce serious changes on the grains and flours, producing in some regions up to 50 % loss. Taking in account the information available up to date, this experiment consists of putting under the effect of the ionizing radiation specimens of Tribolium castaneum feeded with bread flour type 000, with the purpose of controling their biological cycle. They received gamma radiation doses between 250 and 2000 Gy, using 60Co source. The daily observation made over a population of 590 insects, indicates the efficiency of the procedure, non toxic, which provokes the sterility at 250 Gy and inmediate dead starting at 1750 Gy. On the other hand, it was verified that the DL50 on the insects irradiated at the lower of eight different doses applied, reaches 15,3 days, against the 162,6 days of the reference Tribolium. Then it is concluded that it is technologically feasible the application of ionizing radiation to the bread wheat flour type 000 for controling this main plage. (Author)

  1. Walking stability of Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrichidae).

    Science.gov (United States)

    Pires, E M; Nogueira, R M; Pina, D S; Manica, C L M; Faroni, L R A; Moreira, P S A

    2016-04-19

    Results obtained in studies can contribute to the advancement of science and innovative methods and techniques for developing practical activities. Reporting conditions that may restrict the implementation of research is critical to ensure the optimal development of further technical studies. The objective of this study was to assess the walking stability of R. dominica on a flat and smooth surface. The study was based on the determination of mortality, morphology and walking stability of the insect outside the grain mass, on a flat and smooth surface. Mortality of adults of this Coleoptera in conditions with and without food was similar, which explains the difficulty that this insect had for accessing the food source on the flat and smooth surface. The measurements of body length (BOL), width (BOW) and height (BOH) of R. dominica were compared with those of Tribolium castaneum (Coleoptera: Tenebrionidae), which showed good ability to walk in these conditions. This study indicated that the former presents lower BOL and BOW, and greater BOH than the second, and all these variables showed differences when analyzed simultaneously by means of the construction of multivariate morphometric indices (Width × Height, Length × Height and Height × Length × Width). These morphometric variables, together with the definition of the geometry most similar to the body shape, resulted in determination of the center of gravity (CG) and static rollover threshold (SRTgeom) for both species. Rhyzopertha dominica and T. castaneum presented CGs considered high and low, respectively, and together with the values obtained for SRTgeom, may justify that R. dominica can be considered a less stable species during movement, and presents greater risk of rollover on flat and smooth surfaces. PMID:27097085

  2. Anatomical localization and stereoisomeric composition of Tribolium castaneum aggregation pheromones

    Science.gov (United States)

    We report that the abdomen and associated tissues are the predominant sources of male-produced pheromones in the red flour beetle, Tribolium castaneum, and for the first time describe the stereoisomeric composition of the natural blend of isomers of the aggregation pheromone 4,8-dimethyldecanal (DMD...

  3. The genome of the model beetle and pest Tribolium castaneum

    Czech Academy of Sciences Publication Activity Database

    Richards, S.; Jindra, Marek

    2008-01-01

    Roč. 452, č. 7190 (2008), s. 949-955. ISSN 0028-0836 Institutional research plan: CEZ:AV0Z50070508 Keywords : Tribolium castaneum * genome * sequencing Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 31.434, year: 2008

  4. The genome of the model beetle and pest Tribolium castaneum

    DEFF Research Database (Denmark)

    Denell, Robin; Gibbs, Richard; Muzny, Donna;

    2008-01-01

    Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model organism for the study of generalized insect development, and an important pest of stored agricultural products. We describe its genome sequence here. This omnivorous beetle has evolved the ability to inte...

  5. Efeitos dentoesqueléticos produzidos pelo aparelho de Herbst na dentadura mista Dentoskeletal treatment effects produced by the Herbst appliance in the mixed dentition

    Directory of Open Access Journals (Sweden)

    Marcio Rodrigues de Almeida

    2006-10-01

    Full Text Available OBJETIVO: o objetivo desta pesquisa clínica prospectiva foi avaliar as alterações cefalométricas dentárias e esqueléticas produzidas pelo aparelho de Herbst em jovens com má oclusão de Classe II, 1ª divisão durante a dentadura mista. METODOLOGIA: trinta jovens (15 do gênero masculino e 15 do feminino com idade média inicial de 9 anos e 10 meses foram tratados com o aparelho de Herbst por um período de 12 meses. Para a comparação dos grupos utilizou-se uma amostra controle de 30 jovens (15 do gênero masculino e 15 do feminino Classe II, 1ª divisão, com idade média inicial de 9 anos e 8 meses, que foram mantidos sem tratamento durante 12 meses. Para cada jovem foram utilizadas duas telerradiografias em norma lateral, obtidas ao início e no final do período de acompanhamento. Utilizou-se um método convencional de avaliação cefalométrica e o método proposto por Pancherz. RESULTADOS E CONCLUSÕES: os resultados deste estudo demonstraram que os efeitos do aparelho de Herbst produzidos na dentadura mista foram primariamente de natureza dentoalveolar. Os incisivos inferiores foram inclinados para vestibular e os superiores foram retruídos; também houve uma extrusão significante dos molares inferiores, enquanto os superiores sofreram restrição de desenvolvimento no sentido vertical. Não houve diferença significante de restrição do crescimento anterior da maxila entre os dois grupos. No sentido vertical da face, a altura facial ântero-inferior se comportou de forma similar, não demonstrando alteração significante entre os grupos. O tratamento com o aparelho de Herbst produziu um aumento modesto, porém, significante no comprimento da mandíbula comparado ao grupo controle. Este aumento, entretanto, foi de menor magnitude que aquele observado em pacientes adolescentes utilizando o mesmo protocolo de tratamento. A correção do overjet (Herbst ocorreu devido a 22% de alterações esqueléticas e 78% de altera

  6. The Red Flour Beetle, Tribolium castaneum (Coleoptera): A Model for Studies of Development and Pest Biology

    Czech Academy of Sciences Publication Activity Database

    Brown, S. J.; Shippy, T. D.; Miller, S.; Bolognesi, R.; Beemam, R. W.; Lorenzen, M. D.; Bucher, G.; Wimmer, E. A.; Klingler, M.; Posnien, N.; Schinko, J.; Grossmann, D.; Konopová, Barbora; Coleman, C. M.; Tomoyasu, Y.; Kittelmann, S.; Koniszewski, N.; Berghammer, A. J.; Weber, M.; Trauner, J.

    Vol. 2. Plainview: Cold Spring Harbor Laboratory Press, 2010, s. 299-333. ISBN 978-0-87969-872-0 Institutional research plan: CEZ:AV0Z50070508 Keywords : genetics-linkage maps * transgene expression * larval RNAi Subject RIV: EB - Genetics ; Molecular Biology

  7. Clinical effects of fixed functional Herbst appliance in the treatment of class II/1 malocclusion

    Directory of Open Access Journals (Sweden)

    Nedeljković Nenad

    2009-01-01

    Full Text Available Introduction. Sagittal mandible deficiency is the most common cause of skeletal Class II malocclusion. Treatment objective is to stimulate sagittal mandible growth. Fixed functional Herbst appliance use is beneficial for shortening the time required for treatment and does not depend on patient compliance. Case outline. A 13-year-old girl was referred to the Clinic of Orthodontics, School of Dentistry in Belgrade following previous unsuccessful treatment of her skeletal Class II malocclusion using an activator. The patient's poor cooperation had led to failure of the treatment. Patient was subjected to the Herbst treatment for 6 months followed by fixed appliance for another 8 months. Lateral cephalograms before and after the treatment was performed. The remodelation of condylar and fossal articulation was assessed by superimposition of pre- and post-treatment temporomandibular joint tomograms. The promotion of oral hygiene and fluoride use was performed because orthodontic treatment carries a high caries risk and risk for periodontal disease. Skeletal and dental changes were observed after treatment (correction [Max+Mand]: molar relation 7 mm, overjet 8 mm, skeletal relation 5 mm, molars 2 mm, incisors 3 mm. Combination of Herbst and fixed appliances was effective in the treatment of dental and skeletal irregularities for a short period of time. Conclusion . In the retention period, 14 months after treatment, occlusal stability exists. Follow-up care in oral prevention is based on regular recalls at the dental office and supervision at home by the parents.

  8. Orthodontic treatment of nongrowing patient with class II division 2 malocclusion by Herbst appliance

    Directory of Open Access Journals (Sweden)

    Nedeljković Nenad

    2009-01-01

    Full Text Available Background. Inheritance is most casual etiological factor of Class II division 2 malocclusion. This kind of malocclusion is very difficult for treatment specially in older patients. Case report. In the female patient, 20 years old, at the beginning of the treatment at the School of Dentistry in Belgrade, lateral cephalogram showed skeletal and dentoalveolar Class II division 2 malocclusion. She was in the Herbst treatment for 8 months and 12 months more with a fixed multibracket appliance. The measurements were performed on lateral cephalograms before and after the treatment: ii, is, mi, ms, Pg and ss. The distance from these points to occlusal perpendicular line (Olp were measured and compared from cephalogram before to cephalogram after the treatment. Temporomandibular joint (TMJ tomograms were compared from before and after the treatment by superimposition. Correction was found in molar and incisor relation, overjet and overbite. There were found sagital skeletal changes and soft tissue profile improvement. Conclusion. Herbst appliance is effective in the treatment of Class II malocclusions, even in adult patients. Dental and skeletal changes as a result of Herbst treatment could be good choice instead of camouflage orthodontics or surgical decision.

  9. Control of Sitophilus linearis (Herbst) (Coleoptera: Curculionidae) in Tamarindus indica through of gamma radiation of Cobalt-60

    International Nuclear Information System (INIS)

    The objective of this research was to determine the lethal and sterilization dose of gamma radiation of the Cobalt-60 to control adults of Sitophilus linearis in tamarind fruits. For the accomplishment of the experiment fruits of tamarind were disinfested by gamma radiation with a dose of 1,0 kGy. Each treatment consisted of 4 repetition and each one with 10 fruits, in a total of 40 fruits and 40 insects for treatment. The doses of gamma radiation used were: 0 (test), 50, 100, 150 and 200 Gy, with a dose rate of 1,756 kGy/hora. The experiment was kept in a climatic room with temperature of 25+/-3 deg C and relative humidity of 70+/-5%. The evaluation of the mortality of the insects was made every 7 days after the irradiation. The obtained results showed that the dose of 50 Gy was enough to cause the sterilization and lethal dose was 200 Gy to adult insects of this species

  10. UVB Radiation Delays Tribolium castaneum Metamorphosis by Influencing Ecdysteroid Metabolism

    Science.gov (United States)

    Sang, Wen; Yu, Lin; He, Li; Ma, Wei-Hua; Zhu, Zhi-Hui; Zhu, Fen; Wang, Xiao-Ping; Lei, Chao-Liang

    2016-01-01

    Ultraviolet B (UVB) radiation is an important environmental factor. It is generally known that UVB exhibits high genotoxicity due to causing DNA damage, potentially leading to skin carcinogenesis and aging in mammals. However, little is known about the effects of UVB on the development and metamorphosis of insects, which are the most abundant terrestrial animals. In the present study, we performed dose-response analyses of the effects UVB irradiation on Tribolium castaneum metamorphosis, assessed the function of the T. castaneum prothoracicotropic hormone gene (Trcptth), and analyzed ecdysteroid pathway gene expression profile and ecdysterone titers post-UVB irradiation. The results showed that UVB not only caused death of T. castaneum larvae, but also delayed larval-pupal metamorphosis and reduced the size and emergence rate of pupae. In addition, we verified the function of Trcptth, which is responsible for regulating metamorphosis. It was also found that the expression profiles of Trcptth as well as ecdysteroidogenesis and response genes were influenced by UVB radiation. Therefore, a disturbance pulse of ecdysteroid may be involved in delaying development under exposure to irradiation. To our knowledge, this is the first report indicating that UVB can influence the metamorphosis of insects. This study will contribute to a better understanding of the impact of UVB on signaling mechanisms in insect metamorphosis. PMID:26986217

  11. Changes in soft tissue profile following the treatment using a Herbst appliance: A photographic analysis

    Directory of Open Access Journals (Sweden)

    Nedeljković Nenad

    2014-01-01

    Full Text Available Background/Aim. Aesthetics is the reason for most of the class II malocclusion patients to opt for orthodontic treatment. In order to regulate retrognathic profile Herbst appliance for anterior movement of the mandible can be a treatment of choice. The aim of this study was to determine the soft tissue profile changes following Herbst appliance therapy on before and after treatment photos, using the computer program. Methods. This investigation was performed on profile photographs of 20 class II patients (12 females and 8 males aged 18-23 years. Analysis of the changes in soft tissue facial structure relationships evident on the photographs before and after the Herbst appliance therapy was performed using Bentley Micro Station program. The first contour of the soft tissue profile was marked. The following reference lines were subsequently traced: Ricketts aesthetic E line and Juanita line. The area enclosed by these two lines included the nose, upper and lower lip, chin and free space in front of the lips. Using the computer program, the surfaces of the soft tissue structures and free space defined by the reference lines and profile contours were measured. Calculation of the relative proportion of surfaces was done for each photograph. The data obtained were then compared for each patient before and after the treatment. Skeletal and dentoalveolar treatment effects that support soft tissue changes were presented by the profile cephalometric parameters of sagital occlusion (SO analysis. Results. A reduction in the relative surface of the upper lip in males (p < 0.01 and females (p < 0.05 was shown by the pictures. The space occupied by the chin was reduced after the treatment for females only (p < 0.05. The relative surface of the nasal soft tissues, that was included in the reference space was increased (p < 0.01 in both genders. The relationship between the soft tissue and empty surface was changed in favor of the empty surface (p < 0.05 in

  12. Adult Diapause in Coleoptera

    Directory of Open Access Journals (Sweden)

    Ivo Hodek

    2012-01-01

    Full Text Available Recent studies dealing with adult (reproductive diapause in the Coleoptera are reviewed, as a kind of supplement to the classic compendia. In the first two sections, the general characteristics of adult diapause are described and principal terms explained. Original articles dealing with 19 species from nine coleopteran families (Coccinellidae, Chrysomelidae, Bruchidae, Curculionidae, Carabidae, Silphidae, Scolytidae, Scarabaeidae, and Endomychidae are reviewed. Finally attempts are made at generalisations from the papers reviewed, and hypotheses on diapause evolution are inferred. A polyphenic character of diapause is a prominent feature in C. septempunctata and L. decemlineata, but has been found also in other Coleoptera and in insects generally and often generates voltinism heterogeneity within populations.

  13. Identification of candidate chemosensory genes in the antennal transcriptome of Tenebrio molitor (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Liu, Su; Rao, Xiang-Jun; Li, Mao-Ye; Feng, Ming-Feng; He, Meng-Zhu; Li, Shi-Guang

    2015-03-01

    We present the first antennal transcriptome sequencing information for the yellow mealworm beetle, Tenebrio molitor (Coleoptera: Tenebrionidae). Analysis of the transcriptome dataset obtained 52,216,616 clean reads, from which 35,363 unigenes were assembled. Of these, 18,820 unigenes showed significant similarity (E-value SNMP) genes. BLASTX best hit results indicated that these chemosensory genes were most identical to their respective orthologs from Tribolium castaneum. Phylogenetic analyses also revealed that the T. molitor OBPs and CSPs are closely related to those of T. castaneum. Real-time quantitative PCR assays showed that eight TmolOBP genes were antennae-specific. Of these, TmolOBP5, TmolOBP7 and TmolOBP16 were found to be predominantly expressed in male antennae, while TmolOBP17 was expressed mainly in the legs of males. Several other genes were identified that were neither tissue-specific nor sex-specific. These results establish a firm foundation for future studies of the chemosensory genes in T. molitor. PMID:25665775

  14. Odoriferous Defensive stink gland transcriptome to identify novel genes necessary for quinone synthesis in the red flour beetle, Tribolium castaneum.

    Directory of Open Access Journals (Sweden)

    Jianwei Li

    Full Text Available Chemical defense is one of the most important traits, which endow insects the ability to conquer a most diverse set of ecological environments. Chemical secretions are used for defense against anything from vertebrate or invertebrate predators to prokaryotic or eukaryotic parasites or food competitors. Tenebrionid beetles are especially prolific in this category, producing several varieties of substituted benzoquinone compounds. In order to get a better understanding of the genetic and molecular basis of defensive secretions, we performed RNA sequencing in a newly emerging insect model, the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae. To detect genes that are highly and specifically expressed in the odoriferous gland tissues that secret defensive chemical compounds, we compared them to a control tissue, the anterior abdomen. 511 genes were identified in different subtraction groups. Of these, 77 genes were functionally analyzed by RNA interference (RNAi to recognize induced gland alterations morphologically or changes in gland volatiles by gas chromatography-mass spectrometry. 29 genes (38% presented strong visible phenotypes, while 67 genes (87% showed alterations of at least one gland content. Three of these genes showing quinone-less (ql phenotypes - Tcas-ql VTGl; Tcas-ql ARSB; Tcas-ql MRP - were isolated, molecularly characterized, their expression identified in both types of the secretory glandular cells, and their function determined by quantification of all main components after RNAi. In addition, microbe inhibition assays revealed that a quinone-free status is unable to impede bacterial or fungal growth. Phylogenetic analyses of these three genes indicate that they have evolved independently and specifically for chemical defense in beetles.

  15. Anatomical localization and stereoisomeric composition of Tribolium castaneum aggregation pheromones

    Science.gov (United States)

    Lu, Yujie; Beeman, Richard W.; Campbell, James F.; Park, Yoonseong; Aikins, Michael J.; Mori, Kenji; Akasaka, Kazuaki; Tamogami, Shigeyuki; Phillips, Thomas W.

    2011-09-01

    We report that the abdominal epidermis and associated tissues are the predominant sources of male-produced pheromones in the red flour beetle, Tribolium castaneum and, for the first time, describe the stereoisomeric composition of the natural blend of isomers of the aggregation pheromone 4,8-dimethyldecanal (DMD) in this important pest species. Quantitative analyses via gas chromatography-mass spectrometry showed that the average amount of DMD released daily by single feeding males of T. castaneum was 878 ± 72 ng (SE). Analysis of different body parts identified the abdominal epidermis as the major source of aggregation pheromone; the thorax was a minor source, while no DMD was detectable in the head. No internal organs or obvious male-specific glands were associated with pheromone deposition. Complete separation of all four stereoisomers of DMD was achieved following oxidation to the corresponding acid, derivatization with (1 R, 2 R)- and (1 S, 2 S)-2-(anthracene-2,3-dicarboximido)cyclohexanol to diastereomeric esters, and their separation on reversed-phase high-performance liquid chromatography at -54°C. Analysis of the hexane eluate from Porapak-Q-collected volatiles from feeding males revealed the presence of all four isomers (4 R,8 R)/(4 R,8 S)/(4 S,8 R)/(4 S,8 S) at a ratio of approximately 4:4:1:1. A walking orientation bioassay in a wind tunnel with various blends of the four synthetic isomers further indicated that the attractive potency of the reconstituted natural blend of 4:4:1:1 was equivalent to that of the natural pheromone and greater than that of the 1:1 blend of (4 R,8 R)/(4 R,8 S) used in commercial lures.

  16. Microbiota Plays a Role in Oral Immune Priming in Tribolium castaneum

    OpenAIRE

    Futo, Momir; Armitage, Sophie A. O.; Kurtz, Joachim

    2016-01-01

    Animals are inhabited by a diverse community of microorganisms. The relevance of such microbiota is increasingly being recognized across a broad spectrum of species, ranging from sponges to primates, revealing various beneficial roles that microbes can play. The red flour beetle Tribolium castaneum represents a well-established experimental model organism for studying questions in ecology and evolution, however, the relevance of its microbial community is still largely unknown. T. castaneum l...

  17. Primeira ocorrência de Polybius navigator (Herbst) (Decapoda, Brachyura, Portunidae) no Atlântico ocidental First occurrence of Polybius navigator (Herbst) (Decapoda, Brachyura, Portunidae) in Western Atlantic

    OpenAIRE

    Gustavo Augusto Schmidt de Melo; Thais Brandini Crivelaro

    2002-01-01

    Among the portunid crabs collected during the Ilha Grande Project (1966-1969), one specimen of Polybius navigator (Herbst, 1794) was found. This species formerly known from the Eastern Atlantic, Mediterranean and Adriatic and Black Seas is redescribed, illustrated and comments on the new status of the genus is done. It is the first record of this species for the Brazilian coast and Western Atlantic.

  18. Primeira ocorrência de Polybius navigator (Herbst (Decapoda, Brachyura, Portunidae no Atlântico ocidental First occurrence of Polybius navigator (Herbst (Decapoda, Brachyura, Portunidae in Western Atlantic

    Directory of Open Access Journals (Sweden)

    Gustavo Augusto Schmidt de Melo

    2002-03-01

    Full Text Available Among the portunid crabs collected during the Ilha Grande Project (1966-1969, one specimen of Polybius navigator (Herbst, 1794 was found. This species formerly known from the Eastern Atlantic, Mediterranean and Adriatic and Black Seas is redescribed, illustrated and comments on the new status of the genus is done. It is the first record of this species for the Brazilian coast and Western Atlantic.

  19. Dental and orthopedic effects of Bass and Herbst therapy for correction of severe class II division 1 malocclusions

    OpenAIRE

    Ömblus, Jane

    1996-01-01

    Ömblus J (1996). Dental and orthopedic effects of Bass and Herbst therapy for correction of severe Class 1I division l malocclusions. Thesis, Karolinska Institutet. Functional appliance therapy to correct Class II division I malocclusions has been a subject of debate throughout this century. The aims of this thesis, based on six publications, were to evaluate the dental and orthopedic effects of Bass appliance therapy in relation to treatment intensity, age, an...

  20. Effects of Two-Phase Treatment with the Herbst and Preadjusted Edgewise Appliances on the Upper Airway Dimensions

    Science.gov (United States)

    Koay, Woei Li; Tse, Christine Shuk Kwan; Gu, Min

    2016-01-01

    Aims. To assess the effects of two-phase treatment with the Herbst and the preadjusted edgewise appliances on upper airway dimensions and to investigate the correlation between changes in the upper airway dimensions and skeletal morphologies. Methods. A total of 27 Chinese male adolescents aged 12.8 ± 1.3 years were selected. Lateral cephalograms were collected to assess the skeletal morphology and upper airway dimensions. Results. Following Herbst appliance treatment, the upper airway space was significantly enlarged, with the retropalatal (U-MPW) increasing by 1.1 ± 1.6 mm (P 0.05) in upper airway dimensions during the second-phase treatment. Conclusions. Herbst appliance treatment increased the oropharyngeal and hypopharyngeal airway dimensions among adolescents with Class II malocclusion, and the effects were maintained throughout the second treatment phase with a preadjusted edgewise appliance. There was a negative correlation between the change in the depth of the retroglossal pharynx and the mandibular plane angle. PMID:27073805

  1. Structure of the Tribolium castaneum Telomerase Catalytic Subunit TERT

    Energy Technology Data Exchange (ETDEWEB)

    Gillis,A.; Schuller, A.; Skordalakes, E.

    2008-01-01

    A common hallmark of human cancers is the overexpression of telomerase, a ribonucleoprotein complex that is responsible for maintaining the length and integrity of chromosome ends. Telomere length deregulation and telomerase activation is an early, and perhaps necessary, step in cancer cell evolution. Here we present the high-resolution structure of the Tribolium castaneum catalytic subunit of telomerase, TERT. The protein consists of three highly conserved domains, organized into a ring-like structure that shares common features with retroviral reverse transcriptases, viral RNA polymerases and B-family DNA polymerases. Domain organization places motifs implicated in substrate binding and catalysis in the interior of the ring, which can accommodate seven to eight bases of double-stranded nucleic acid. Modelling of an RNA-DNA heteroduplex in the interior of this ring demonstrates a perfect fit between the protein and the nucleic acid substrate, and positions the 3'-end of the DNA primer at the active site of the enzyme, providing evidence for the formation of an active telomerase elongation complex.

  2. Expression patterns of cysteine peptidase genes across the Tribolium castaneum life cycle provide clues to biological function

    Science.gov (United States)

    The red flour beetle, Tribolium castaneum, is a major agricultural pest responsible for considerable loss of stored grain and cereal products worldwide. T. castaneum larvae have a highly compartmentalized gut, with cysteine peptidases mostly in the acidic anterior part of the midgut. We have descri...

  3. The natural diet of the mud crab Scylla olivacea (Herbst, 1896) in Pichavaram mangroves, India.

    Science.gov (United States)

    Viswanathan, C; Raffi, S M

    2015-11-01

    Food and feeding habits of mud crab Scylla olivacea (Herbst, 1896) in Pichavaram mangroves was investigated quantitatively and qualitatively for a period of two years from June 2010 to May 2012. Gut contents from 1737 specimens comprising 843 males and 894 females in the size range between 45 mm and 148 mm were examined. Crustaceans form the predominant food item in a majority of size groups in terms of percentage wet weight and frequency of occurrence, while molluscs showed a preference in few size groups. The other dietary items includes fishes, detritus, mud and sand and miscellaneous. Gut content analysis revealed no significant variation between the quantities of food consumed by both sexes. Feeding intensity was higher in juveniles and subadults of both sexes than that of adults, revealing a greater preference to feed on fast moving prey such as crustaceans and fishes. The results of the present study indicate that S. olivacea in Pichavaram mangroves exhibited a clear preference for crustaceans. PMID:26586996

  4. Antifeedant Diterpenoids against Tribolium castaneum from the Stems and Twigs of Ceriops tagal (Rhizophoraceae

    Directory of Open Access Journals (Sweden)

    Zhi Wei Deng

    2011-07-01

    Full Text Available The screening of several Chinese mangrove plants for insecticidal principles showed that ethanol extract of Ceriops tagal stems and twigs possessed significant feeding deterrent activity against the red flour beetle, Tribolium castaneum (Family: Rhizophoraceae. From the ethanol extract, three feeding deterrent diterpenoids were isolated by bioassay-guided fractionation. The compounds were identified as tagalsin A, B, and H on the basis of their phytochemical and spectral data. Tagalsin A, B, and H exhibited strong feeding deterrent activity against T. castaneum adults with EC50 values of 375.3 ppm, 277.3 ppm, and 285.45 ppm, respectively.

  5. Gene Families of Cuticular Proteins Analogous to Peritrophins (CPAPs) in Tribolium castaneum Have Diverse Functions

    OpenAIRE

    Jasrapuria, Sinu; Specht, Charles A.; Kramer, Karl J.; Beeman, Richard W.; Muthukrishnan, Subbaratnam

    2012-01-01

    The functional characterization of an entire class of 17 genes from the red flour beetle, Tribolium castaneum, which encode two families of Cuticular Proteins Analogous to Peritrophins (CPAPs) has been carried out. CPAP genes in T. castaneum are expressed exclusively in cuticle-forming tissues and have been classified into two families, CPAP1 and CPAP3, based on whether the proteins contain either one (CPAP1), or three copies (CPAP3) of the chitin-binding domain, ChtBD2, with its six characte...

  6. Family-Group Names In Coleoptera (Insecta

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    Patrice Bouchard

    2011-04-01

    Full Text Available We synthesize data on all known extant and fossil Coleoptera family-group names for the first time. A catalogue of 4887 family-group names (124 fossil, 4763 extant based on 4707 distinct genera in Coleoptera is given. A total of 4492 names are available, 183 of which are permanently invalid because they are based on a preoccupied or a suppressed type genus. Names are listed in a classification framework. We recognize as valid 24 superfamilies, 211 families, 541 subfamilies, 1663 tribes and 740 subtribes. For each name, the original spelling, author, year of publication, page number, correct stem and type genus are included. The original spelling and availability of each name were checked from primary literature. A list of necessary changes due to Priority and Homonymy problems, and actions taken, is given. Current usage of names was conserved, whenever possible, to promote stability of the classification. New synonymies (family-group names followed by genus-group names: Agronomina Gistel, 1848 syn. n. of Amarina Zimmermann, 1832 (Carabidae, Hylepnigalioini Gistel, 1856 syn. n. of Melandryini Leach, 1815 (Melandryidae, Polycystophoridae Gistel, 1856 syn. n. of Malachiinae Fleming, 1821 (Melyridae, Sclerasteinae Gistel, 1856 syn. n. of Ptilininae Shuckard, 1839 (Ptinidae, Phloeonomini Ádám, 2001 syn. n. of Omaliini MacLeay, 1825 (Staphylinidae, Sepedophilini Ádám, 2001 syn. n. of Tachyporini MacLeay, 1825 (Staphylinidae, Phibalini Gistel, 1856 syn. n. of Cteniopodini Solier, 1835 (Tenebrionidae; Agronoma Gistel 1848 (type species Carabus familiaris Duftschmid, 1812, designated herein syn. n. of Amara Bonelli, 1810 (Carabidae, Hylepnigalio Gistel, 1856 (type species Chrysomela caraboides Linnaeus, 1760, by monotypy syn. n. of Melandrya Fabricius, 1801 (Melandryidae, Polycystophorus Gistel, 1856 (type species Cantharis aeneus Linnaeus, 1758, designated herein syn. n. of Malachius Fabricius, 1775 (Melyridae, Sclerastes Gistel, 1856 (type species

  7. Forward genetics in Tribolium castaneum: opening new avenues of research in arthropod biology

    OpenAIRE

    Peel, Andrew D

    2009-01-01

    A recent paper in BMC Biology reports the first large-scale insertional mutagenesis screen in a non-drosophilid insect, the red flour beetle Tribolium castaneum. This screen marks the beginning of a non-biased, 'forward genetics' approach to the study of genetic mechanisms operating in Tribolium. See research article http://biomedcentral.com/1741-7007/7/73

  8. Tribolium castaneum defensins are primarily active against Gram-positive bacteria

    Czech Academy of Sciences Publication Activity Database

    Tonk, M.; Knorr, E.; Cabezas-Cruz, A.; Valdés, James J.; Kollewe, C.; Vilcinskas, A.

    2015-01-01

    Roč. 132, NOV 2015 (2015), s. 208-215. ISSN 0022-2011 R&D Projects: GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : Antimicrobial peptides * Defensin * Innate immunity * Insects * Tribolium castaneum * Gram-positive bacteria Subject RIV: EI - Biotechnology ; Bionics Impact factor: 2.110, year: 2014

  9. DIRS retroelements in arthropods: identification of the recently active TcDirs1 element in the red flour beetle Tribolium castaneum.

    Science.gov (United States)

    Goodwin, T J D; Poulter, R T M; Lorenzen, M D; Beeman, R W

    2004-08-01

    Members of the DIRS family of retrotransposons differ from most other known retrotransposons in that they encode a tyrosine recombinase (YR), a type of enzyme frequently involved in site-specific recombination. This enzyme is believed to insert the extrachromosomal DNA intermediate of DIRS element retrotransposition into the host genome. DIRS elements have been found in plants, a slime mold, fungi, and a variety of animals including vertebrates, echinoderms and nematodes. They have a somewhat patchy distribution, however, apparently being absent from a number of model organisms such as Saccharomyces cerevisiae, Arabidopsis thaliana and Drosophila melanogaster. In this report we describe the first DIRS retroelement to be identified in an arthropod. This element, TcDirs1, was found in the red flour beetle Tribolium castaneum (Coleoptera). It is generally similar in sequence and structure to several previously described members of the DIRS group: it is bordered by inverted terminal repeats and it has a similar set of protein-coding domains (Gag, reverse transcriptase/ribonuclease H, and the YR), although these are arranged in a novel fashion. TcDirs1 elements exhibit several features indicative of recent activity, such as intact coding regions, a high level of sequence similarity between distinct elements and polymorphic insertion sites. Given their presence in an experimentally tractable host, these potentially active elements might serve as useful models for the study of DIRS element retrotransposition. An element closely related to TcDirs1 was also detected in sequences from a second arthropod, the honey bee Apis mellifera (Hymenoptera), suggesting that these retrotransposons are long-term residents of arthropod genomes. PMID:15221458

  10. ANTHONOMUS RUBI (HERBST, 1795 (COLEOPTERA: CURCULIONIDAE A NEW DANGEROUS PEST IN THE ECOLOGICAL CROPS OF STRAWBERRY IN THE SOUTHERN REGIONS OF ROMANIA

    Directory of Open Access Journals (Sweden)

    Traian MANOLE

    2013-12-01

    Full Text Available In Romania mostly of 70% of strawberry ecological plantations were cultivated with specific commercial cultivars like Premial, Red Gauntlet, Dana and Elsanta. In the last years became to be cropped cultivars with increased resistance to various pest insects, specially in the first stages of vegetation period like Honeoye, Elsanta, Camorosa, Marmolada, Chandler, Darselect, Clery and Sonata. On the little individual farms with small surfaces the cultivars Senga Sengana, Marmolada, Elsinore should be the primary source of infestation with Anthonomus rubi.A study of distribution, density and the degree attack of Anthonomus rubi was carried out in the period 2006-2007 in the Vidra and Comana localities, Giurgiu district in the southern part of Romania. The study and observations that was made showed that this species is increased in density in the new ecological plantations of strawberry from some individual farms. The strawberry blossom weevil is not a common pest of strawberry plantations in Romania but some cultivars cropped in ecological system became more sensitive to the weevil attack by increasing in density. Observations and researches were carried out on 4 cultivars and showed that the degree of damage caused by A. rubi depends of cultivar morphological characteristics (small fruits or phenophases (time of ripening and duration of budding and blossoming.

  11. Avaliação da quantidade de movimentação dos molares superiores com emprego do aparelho de Herbst Assessment of the displacement of the upper molars using the Herbst appliance

    Directory of Open Access Journals (Sweden)

    Paulo Cezar Rodrigues Ogeda

    2004-08-01

    Full Text Available O Herbst é um aparelho ortopédico funcional fixo destinado principalmente ao estímulo de crescimento mandibular durante a correção da má oclusão de Classe II esquelética de pacientes em crescimento. Várias publicações descreveram os efeitos deste aparelho durante a correção da Classe II, revelando a promoção de alterações esqueléticas e dentárias em igual proporção, favorecendo sua correção. Parte do movimento dentário ocorre por distalização dos primeiros molares superiores. Esta pesquisa teve como objetivo avaliar a quantidade e o tipo de movimento distal ocorrido com os primeiros molares superiores permanentes, e a conseqüência destes movimentos sobre o plano oclusal funcional durante o período de utilização do aparelho de Herbst. A amostra foi composta de 22 pacientes portadores da má oclusão de classe II esquelética, retrognatismo mandibular, com idade média de 12 anos e 11 meses, tratados por um período médio de 10,1 meses. Os aparelhos foram construídos utilizando a ancoragem total no arco maxilar, visando potencializar a ação ortopédica e minimizar a perda de ancoragem. As alterações foram medidas em cefalogramas específicos obtidos das telerradiografias em norma lateral tomadas em dois tempos: tempo 1 (T1 antes da instalação do aparelho, e tempo 2 (T2, após sua remoção. Medidas cefalométricas lineares e angulares em relação ao plano horizontal de Frankfurt, plano palatino e a uma linha vertical de referência a partir do ponto S perpendicular a Frankfurt, foram utilizadas para quantificar os deslocamentos dos primeiros molares superiores. Foram avaliados: o deslocamento distal médio das coroas, o deslocamento distal médio de suas raízes, a conseqüente inclinação no longo eixo dos molares durante a distalização, o deslocamento vertical em relação ao plano palatino, e finalmente, a conseqüência da variação vertical do primeiro molar sobre o plano oclusal funcional. Os

  12. Addenda and Corrigenda to the Catalogue of Palaearctic Coleoptera, volumes 7 and 8 (Curculionoidea

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    Alonso-Zarazaga, Miguel A.

    2016-06-01

    Full Text Available Additions, corrections, comments and nomenclatural novelties for the volumes 7 and 8 of the Catalogue of Palaearctic Coleoptera are provided. For the exact authorship of these check the text. One new species is described: Mecinus tavaresi Caldara & Fogato, sp. nov. from Portugal and Spain. New synonymies are: Compsapoderus (Compsapoderus erythropterus (Gmelin, 1790 = Attelabus intermedius Hellwig, 1795, syn. nov.; Paroplapoderus (Erycapoderus angulipennis (Kolbe, 1886 = Paroplapoderus (Erycapoderus angulipennis shaanxinsis Legalov, 2004, syn. nov. (Attelabidae; Aspidapion (Koestlinia aeneum (Fabricius, 1775 = Aspidapion (Koestlinia motschulskyi (Hochhuth, 1847, syn. nov., Taeniapion rufescens (Gyllenhal, 1833 = Taeniapion notatum (Wagner, 1912, syn. nov. (Apionidae; Larinus (Larinomesius scolymi (Olivier, 1807 = Curculio teres Hellwig, 1795, syn. nov. (Curculionidae; Lixus paraplecticus (Linnaeus, 1758 = Curculio phellandrii Linnaeus, 1764, syn. nov. (Curculionidae; Curculio alternans Hellwig, 1795= Curculio alternans Herbst, 1795, syn. nov. (Curculionidae; Sibinia lyrata Faust, 1889= Sibinia attalica var. judea Pic, 1901, syn. nov. (Curculionidae; Phyllobius (Metaphyllobius pomaceus Gyllenhal, 1834= Curculio prasinus Olivier, 1791, syn. nov. (Curculionidae. New homonymies are: Attelabus intermedius Hellwig, 1795 (non Attelabus intermedius Illiger, 1794; Baris marshalli Ramesha & Ramamurthy, 2011 (non Baris marshalli Hustache, 1938. New replacement names are: Baris ramamurthyi Alonso-Zarazaga nom. nov. for Baris bimaculata Pajni & Kohli, 1990 (non Hustache, 1932; Archarius (Archarius kwonleeanus Alonso-Zarazaga nom. nov. for Archarius (Archarius parvus (Kwon & Lee, 1990 (non Archarius (Archarius parvus (Hong & Wang, 1987, a fossil species; Curculio (Curculio zhangianus Alonso-Zarazaga nom. nov. for Curculio (Curculio helleri Pelsue & Zhang, 2002 (non Curculio (Curculio helleri (Voss, 1932; Lixus trichromus Alonso-Zarazaga nom. nov. for Lixus

  13. Microbiota plays a role in oral immune priming in Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Momir eFuto

    2016-01-01

    Full Text Available Animals are inhabited by a diverse community of microorganisms. The relevance of such microbiota is increasingly being recognised in a broad spectrum of species, ranging from sponges to primates, revealing various beneficial roles that microbes can play. The red flour beetle Tribolium castaneum represents a well-established experimental model organism for studying questions in ecology and evolution, however, the relevance of its microbial community is still largely unknown. T. castaneum larvae orally exposed to inactivated bacterial components of the entomopathogen Bacillus thuringiensis bv. tenebrionis showed increased survival upon a subsequent challenge with spores of this bacterium. To investigate whether T. castaneum microbiota plays a role in this phenomenon, we established a protocol for raising microbe-free larvae and subsequently tested whether they differ in their ability to mount such a priming response. Here we demonstrate that larvae with significantly lowered microbial loads, show decreased survival upon secondary challenge with B. thuringiensis bv. tenebrionis spores, compared to animals which were allowed to regain their microbiota before priming. Although the exact mechanism of oral immune priming is unclear, we here suggest that microbiota plays a crucial role in oral immune priming in this species.

  14. Repellent Constituents of essential oil from Citrus wilsonii stem barks against Tribolium castaneum.

    Science.gov (United States)

    Wua, Yan; Chenb, Hai-Ping; Wei, Jian-Yu; Yang, Kai; Tian, Zhao-Fu; Li, Xiao-Lan; Wang, Ping-Juan; Wang, Cheng-Fang; Du, Shu-Shan; Cai, Qian

    2014-10-01

    The essential oil obtained from Citrus wilsonii Tanaka stem barks with hydrodistillation was investigated by GC-FID and GC-MS. The main components of the essential oil were identified to be nerol acetate (44.5%), nerol (13.6%), citronellyl propionate (13.5%) and α-terpineol (3.6%). Among them, the four active constituents, predicted with a bioactivity-test, were isolated and identified as nerolacetate, nerol, citronellyl propionate and α-terpineol. It was found that the essential oil of C. wilsonii stem barks possessed strong repellency (86% and 92%, respectively, at 78.6 nL/cm2, after 2 and 4 h treatment) against Tribolium castaneum adults. Repellency of the four active compounds was also determined. Nerolacetate, nerol, citronellyl propionate and α-terpineol were strongly repellent (100%, 100%, 90% and 96%, respectively, at 15.7 nL/cm2, after 2h treatment) against T. castaneum. Nerol exhibited the same level of repellency as the positive control, DEET. The results indicate that the essential oil of C. wilsonii stem barks and its active compounds have the potential to be developed as natural repellents for control of T. castaneum. PMID:25522550

  15. Complete sequence and characterization of mitochondrial genome in the swimming crab Portunus sanguinolentus (Herbst, 1783) (Decapoda, Brachyura, Portunidae).

    Science.gov (United States)

    Meng, Xianliang; Jia, Fulong; Zhang, Xiaohui; Liu, Ping; Li, Jian

    2016-07-01

    The three-spot swimming crab Portunus sanguinolentus (Herbst, 1783) is a commercially important fishery species, widely distributed in the Indo-Pacific region. In this study, we present the complete mitochondrial genome of P. sanguinolentus. The genome is 16 027 bp in length with circular organization, encoding the standard set of 13 protein-coding genes, 22 tRNA genes, and two rRNA genes. The overall A + T content is 65.60%, which is lower than that of its congeneric species Portunus pelagicus and Portunus trituberculatus. The mitogenome carries 1254 bp of intergenic region constituting 7.82% of the genome, and six pairs of overlapping genes with the overlap size from 1 to 7 bp. The complete mitogenome sequence information of P. sanguinolentus would provide useful data for further studies on population genetics and molecular systematics. PMID:26153754

  16. The role of the serosa in innune defense of the beetle Tribolium castaneum

    OpenAIRE

    Bernardes, Joana Pimenta, 1988-

    2012-01-01

    Tese de mestrado. Biologia (Biologia Evolutiva e do Desenvolvimento). Universidade de Lisboa, Faculdade de Ciências, 2012 O modelo de investigação Tribolium castaneum tem sido amplamente utilizado em áreas como a biologia do desenvolvimento e evolução. Os trabalhos de investigação nesta espécie são importantes devido à sua relevância em termos económicos e de controlo de pragas. Esta espécie está inserida na ordem Coleopetera, a ordem com maior diversidade biológica conhecida. Este modelo ...

  17. A genome-wide inventory of neurohormone GPCRs in the red flour beetle Tribolium castaneum

    DEFF Research Database (Denmark)

    Hauser, Frank; Cazzamali, Giuseppe; Williamson, Michael;

    2008-01-01

    Insect neurohormones (biogenic amines, neuropeptides, and protein hormones) and their G protein-coupled receptors (GPCRs) play a central role in the control of behavior, reproduction, development, feeding and many other physiological processes. The recent completion of several insect genome...... consumption. In addition, T. castaneum is a model for insect development. Here, we have investigated the presence of neurohormone GPCRs in Tribolium and compared them with those from the fruit fly Drosophila melanogaster (Diptera) and the honey bee Apis mellifera (Hymenoptera). We found 20 biogenic amine...

  18. Effectiveness of gamma radiation for the control of tribolium castaneum, the pest of stored cashew kernels

    International Nuclear Information System (INIS)

    Effect of gamma radiation on T. castaneum irradiated during adult stage revealed that survival of adults was found to decrease with increase in radiation dose. Gamma radiations are used to produce mortality or sterility in the insects. The technique can be used by irradiating the insects at doses sufficiently high to produce the desired effects. The present study observed the gamma-ray effect on this insect, and the results may be useful for commercial applications. Overall the results showed highly significant effect of irradiation on insects administered with different doses

  19. Coleoptera Associated with Decaying Wood in a Tropical Deciduous Forest.

    Science.gov (United States)

    Muñoz-López, N Z; Andrés-Hernández, A R; Carrillo-Ruiz, H; Rivas-Arancibia, S P

    2016-08-01

    Coleoptera is the largest and diverse group of organisms, but few studies are dedicated to determine the diversity and feeding guilds of saproxylic Coleoptera. We demonstrate the diversity, abundance, feeding guilds, and succession process of Coleoptera associated with decaying wood in a tropical deciduous forest in the Mixteca Poblana, Mexico. Decaying wood was sampled and classified into four stages of decay, and the associated Coleoptera. The wood was identified according to their anatomy. Diversity was estimated using the Simpson index, while abundance was estimated using a Kruskal-Wallis test; the association of Coleoptera with wood species and decay was assessed using canonical correspondence analysis. Decay wood stage I is the most abundant (51%), followed by stage III (21%). We collected 93 Coleoptera belonging to 14 families, 41 genera, and 44 species. The family Cerambycidae was the most abundant, with 29% of individuals, followed by Tenebrionidae with 27% and Carabidae with 13%. We recognized six feeding guilds. The greatest diversity of Coleoptera was recorded in decaying Acacia farnesiana and Bursera linanoe. Kruskal-Wallis analysis indicated that the abundance of Coleoptera varied according to the species and stage of decay of the wood. The canonical analysis showed that the species and stage of decay of wood determined the composition and community structure of Coleoptera. PMID:26911160

  20. Functional analysis of the ATP-binding cassette (ABC transporter gene family of Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Broehan Gunnar

    2013-01-01

    Full Text Available Abstract Background The ATP-binding cassette (ABC transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. Most are integral membrane proteins that transport a broad spectrum of substrates across lipid membranes. In insects, ABC transporters are of special interest because of their role in insecticide resistance. Results We have identified 73 ABC transporter genes in the genome of T. castaneum, which group into eight subfamilies (ABCA-H. This coleopteran ABC family is significantly larger than those reported for insects in other taxonomic groups. Phylogenetic analysis revealed that this increase is due to gene expansion within a single clade of subfamily ABCC. We performed an RNA interference (RNAi screen to study the function of ABC transporters during development. In ten cases, injection of double-stranded RNA (dsRNA into larvae caused developmental phenotypes, which included growth arrest and localized melanization, eye pigmentation defects, abnormal cuticle formation, egg-laying and egg-hatching defects, and mortality due to abortive molting and desiccation. Some of the ABC transporters we studied in closer detail to examine their role in lipid, ecdysteroid and eye pigment transport. Conclusions The results from our study provide new insights into the physiological function of ABC transporters in T. castaneum, and may help to establish new target sites for insect control.

  1. Tanyproctini (Coleoptera: Scarabaeidae: Melolonthinae) of Socotra Island

    Czech Academy of Sciences Publication Activity Database

    Král, D.; Sehnal, R.; Bezděk, Aleš

    2012-01-01

    Roč. 52, suppl. 2 (2012), s. 153-182. ISSN 0374-1036 Grant ostatní: Ministry of Education, Youth and Sports (CZ) LA10036/MSMT Institutional support: RVO:60077344 Keywords : Coleoptera * Scarabaeidae * Melolonthinae Subject RIV: EG - Zoology Impact factor: 0.963, year: 2012 http://www.aemnp.eu/PDF/52_s2/52_S2_153.pdf

  2. Escarabeíneos copro-necrófagos (Coleoptera, Scarabaeidae, Scarabaeinae de fragmentos de Mata Atlântica em Silveira Martins, Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Pedro G. da Silva

    2012-06-01

    Full Text Available A fauna de Scarabaeinae (Coleoptera: Scarabaeidae foi amostrada através de armadilhas de queda iscadas com excremento humano e peixe apodrecido em fragmentos florestais de Silveira Martins, Rio Grande do Sul, Brasil, de novembro de 2010 a janeiro de 2011. Foi coletado um total de 1.611 indivíduos, pertencentes a seis tribos, 11 gêneros e 28 espécies. As espécies mais abundantes foram Canthon latipes Blanchard, 1845 (49,9%, C. chalybaeus Blanchard, 1845 (13,9%, Deltochilum sculpturatum Felsche, 1907 (4,9% e Eurysternus caribaeus (Herbst, 1789 (4,3%, que juntas representaram 73% do total de indivíduos capturados. As armadilhas iscadas com excremento humano capturaram maior número de espécies do que as iscadas com peixe apodrecido. Não houve diferença estatística significativa entre os tipos de iscas utilizados em relação à abundância de Scarabaeinae. A maior parte da comunidade de Scarabaeinae capturada foi representada por espécies de hábito alimentar generalista e comportamento escavador. A comunidade amostrada segue os padrões gerais de estrutura trófica e comportamental de Scarabaeinae encontrados por toda a região Neotropical.

  3. Effects of γ-irradiation on larval and adult stages of Tribolium castaneum (red flour beetle)

    International Nuclear Information System (INIS)

    Food irradiation using γ-rays is one of powerful tools to reduce insect infestation in crops and spices. We examined the effects of γ-irradiation on larvae and adults of Tribolium castaneum. Eclosion for larvae was completely inhibited by irradiation at 500 Gy. A survival rate for adults was markedly reduced by irradiation at more than 500 Gy. The neutral comet assay presented that irradiation irreversibly increased DNA damage at 500 and 1000 Gy and transiently increased DNA damage at 100 Gy, suggesting possible DNA repair in larvae and adults stages. These results indicate that γ-irradiation at 500 Gy is available to eradicate the beetle at larval and adults stages. (author)

  4. Genome-wide mapping of conserved microRNAs and their host transcripts in Tribolium castaneum

    Institute of Scientific and Technical Information of China (English)

    Qibin Luo; Qing Zhou; Xiaomin Yu; Hongbin Lin; Songnian Hu; Jun Yu

    2008-01-01

    MicroRNAs (miRNAs) are endogenous 22-nt RNAs, which play important regulatory roles by post-transcriptional gene silencing. A computational strategy has been developed for the identification of conserved miRNAs based on features of known metazoan miRNAs in red flour beetle (Tribolium castaneum), which is regarded as one of the major laboratory models of arthropods. Among 118 putative miRNAs, 47% and 53% of the predicted miRNAs from the red flour beetle are harbored by known protein-coding genes (intronic) and genes located outside (intergenic miRNA), respectively. There are 31 intronic miRNAs in the same transcriptional orientation as the host genes, which may share RNA polymerase Ⅱ and spliceosomal machinery with their host genes for their biogenesis. A hypothetical feedback model has been proposed based on the analysis of the relationship between intronic miRNAs and their host genes in the development of red flour beetle.

  5. MicroRNA evolution, expression, and function during short germband development in Tribolium castaneum.

    Science.gov (United States)

    Ninova, Maria; Ronshaugen, Matthew; Griffiths-Jones, Sam

    2016-01-01

    MicroRNAs are well-established players in the development of multicellular animals. Most of our understanding of microRNA function in arthropod development comes from studies in Drosophila. Despite their advantages as model systems, the long germband embryogenesis of fruit flies is an evolutionary derived state restricted to several holometabolous insect lineages. MicroRNA evolution and expression across development in animals exhibiting the ancestral and more widespread short germband mode of embryogenesis has not been characterized. We sequenced small RNA libraries of oocytes and successive intervals covering the embryonic development of the short germband model organism, Tribolium castaneum. We analyzed the evolution and temporal expression of the microRNA complement and sequenced libraries of total RNA to investigate the relationships with microRNA target expression. We show microRNA maternal loading and sequence-specific 3' end nontemplate oligoadenylation of maternally deposited microRNAs that is conserved between Tribolium and Drosophila. We further uncover large clusters encoding multiple paralogs from several Tribolium-specific microRNA families expressed during a narrow interval of time immediately after the activation of zygotic transcription. These novel microRNAs, together with several early expressed conserved microRNAs, target a significant number of maternally deposited transcripts. Comparison with Drosophila shows that microRNA-mediated maternal transcript targeting is a conserved process in insects, but the number and sequences of microRNAs involved have diverged. The expression of fast-evolving and species-specific microRNAs in the early blastoderm of T. castaneum is consistent with previous findings in Drosophila and shows that the unique permissiveness for microRNA innovation at this stage is a conserved phenomenon. PMID:26518483

  6. Host plant preference in Colorado potato beetle (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Field and laboratory-choice tests were conducted to better understand host plant preference by the Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), in Virginia. In laboratory olfactometer studies, L. decemlineata preferred potato over both tomato and eggplant foli...

  7. Insulin receptor regulates food intake through sulfakinin signaling in the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Lin, Xianyu; Yu, Na; Smagghe, Guy

    2016-06-01

    Insects obtain energy and nutrients via feeding to support growth and development. The insulin signaling pathway is involved in the regulation of feeding; however, the underlying mechanisms are not fully understood. Here, we show that insulin signaling regulates food intake via crosstalk with neuropeptide sulfakinin in the red flour beetle, Tribolium castaneum. Silencing of the insulin receptor (InR) decreased the food intake in the penultimate and final instar stages, leading to a decrease of weight gain and mortality during larval-pupal metamorphosis. Interestingly, the knockdown of InR co-occurred with an increased expression of sulfakinin (sk), a gene encoding neuropeptide SK functioning as a satiety signal. In parallel, double silencing of sk and InR eliminated the inhibitory effect on food intake as induced by silencing of InR and the larvae died as prepupae. In conclusion, this study shows, for the first time, that the insulin/InR signaling regulates food intake through the sulfakinin signaling pathway in the larval stages of this important model and pest insect, indicating a novel target for pest control. PMID:26972481

  8. Estudo cefalométrico das alterações dentoesqueléticas da má oclusão de Classe II, divisão 1 tratada com o aparelho de Herbst com cantiléver Cephalometric study in patients that displayed Class II, division 1 malocclusion treated with herbst appliance

    OpenAIRE

    Ana Carla Raphaelli Nahás; José Fernando Castanha Henriques; Guilherme Janson; Bryan D. Tompson; Donald G. Woodside

    2008-01-01

    OBJETIVO: Realizou-se um estudo cefalométrico, em telerradiografias, objetivando-se determinar os efeitos no complexo craniofacial de pacientes com más oclusões de Classe II, divisão 1 submetidos ao tratamento com o aparelho de Herbst com cantiléver. METODOLOGIA: Para tanto, utilizou-se uma amostra composta por dois grupos, sendo um experimental e um controle. O grupo experimental originou-se da Faculdade de Odontologia de Bauru - USP, com 25 pacientes tratados com o aparelho ortopédico funci...

  9. ON THE TAXONOMY AND NOMENCLATURE OF SOME MECININI (COLEOPTERA, CURCULIONIDAE

    Directory of Open Access Journals (Sweden)

    Roberto Caldara

    2008-04-01

    Full Text Available In accordance with the Code, ten actions are taken to preserve nomenclatural stability of names of taxa currently belonging to Mecinini. Following the provisions of ICZN Article 23.9.1, Cleopomiarus graminis (Gyllenhal, 1813 (formerly Rhynchaenus is made a nomen protectum and Curculio ellipticus Herbst, 1795 is made a nomen oblitum; Rhinusa antirrhini (Paykull, 1800 (formerly Curculio is made a nomen protectum and Curculio noctis herbst, 1795 is made a nomen oblitum; having met the conditions of ICZN article 75.3 the neotypes of the following taxa are designated: Curculio antirrhini Paykull, 1800, Curculio cinctus Rossi, 1790, Curculio curvirostris Rossi, 1790, Curculio linariae Panzer, 1792, Cionus thapsicola Germar, 1821, Mecinus collaris Germar, 1821. Lectotypes of Curculio ellipticus Herbst, 1795, Gymnetron eversmanni Rosenschöld, 1838, Mecinus barbarus Gyllenhal, 1838, and Mecinus longiusculus Boheman, 1845 are also designated. Rhinusa linariae (Panzer, 1792 (formerly Curculio remains the valid name of the taxon since Curculio curvirostris Rossi, 1790 (non Fabricius, 1781 nec Herbst, 1784 is unavailable; Mecinus collaris Germar, 1821 remains the valid name of the taxon since Curculio cinctus Rossi, 1790 (non Drury, 1782 nec Geoffroy, 1785 is unavailable. The following new synonymies are proposed: Mecinus barbarus Gyllenhal, 1838 = Mecinus longiusculus Boheman, 1845 n. syn., = Mecinus teretiusculus Boheman, 1845 n. syn., = Mecinus filiformis Aubé, 1850 n. syn.; Rhinusa florum (Rübsaamen, 1895 = Gymnetron smreczynskii Fremuth, 1972 n. syn.; Rhinusa tetra (Fabricius, 1792 = Cionus thapsicola Germar, 1821 n. syn. Rhinusa eversmanni (Rosenschöld, 1838 is the name proposed for Rhinusa thapsicola sensu auctorum (non Germar, 1821.

  10. O escaravelho Megasoma gyas (Herbst, 1775, espécie ameaçada de extinção, no Parque Estadual Carlos Botelho, Sete Barras – SP. The elephant beetle Megasoma gyas (Herbst, 1775, threatened species, in Carlos Botelho State Park, Sete Barras, SP, Brazil.

    Directory of Open Access Journals (Sweden)

    Alexsander Zamorano ANTUNES

    2007-12-01

    Full Text Available As espécies do gênero Megasoma sãobesouros naturalmente raros e pouco conhecidos.Megasoma gyas (Herbst, 1775, espécie restrita aoterritório brasileiro e considerada ameaçada deextinção, foi encontrada no Parque Estadual CarlosBotelho em maio de 2007. O presente trabalhorevisa a distribuição da espécie no Estado de SãoPaulo, detalha a sua simpatria com outra espéciedo gênero e discute aspectos comportamentais eameaças à sua sobrevivência.The elephant beetle Megasoma spp. arenaturally scarce and poorly known species.Megasoma gyas (Herbst, 1775, Brazilian endemicand threatened species, was recorded in CarlosBotelho State Park, in May 2007. The speciesdistribution in the State of São Paulo, southeasternBrazil, is reviewed and the simpatry with anotherMegasoma species, behavioral aspects and threatsfaced by this species are shown.

  11. Glycogen and glucose metabolism are essential for early embryonic development of the red flour beetle Tribolium castaneum.

    Directory of Open Access Journals (Sweden)

    Amanda Fraga

    Full Text Available Control of energy metabolism is an essential process for life. In insects, egg formation (oogenesis and embryogenesis is dependent on stored molecules deposited by the mother or transcribed later by the zygote. In oviparous insects the egg becomes an isolated system after egg laying with all energy conversion taking place during embryogenesis. Previous studies in a few vector species showed a strong correlation of key morphogenetic events and changes in glucose metabolism. Here, we investigate glycogen and glucose metabolism in the red flour beetle Tribolium castaneum, an insect amenable to functional genomic studies. To examine the role of the key enzymes on glycogen and glucose regulation we cloned and analyzed the function of glycogen synthase kinase 3 (GSK-3 and hexokinase (HexA genes during T. castaneum embryogenesis. Expression analysis via in situ hybridization shows that both genes are expressed only in the embryonic tissue, suggesting that embryonic and extra-embryonic cells display different metabolic activities. dsRNA adult female injection (parental RNAi of both genes lead a reduction in egg laying and to embryonic lethality. Morphological analysis via DAPI stainings indicates that early development is impaired in Tc-GSK-3 and Tc-HexA1 RNAi embryos. Importantly, glycogen levels are upregulated after Tc-GSK-3 RNAi and glucose levels are upregulated after Tc-HexA1 RNAi, indicating that both genes control metabolism during embryogenesis and oogenesis, respectively. Altogether our results show that T. castaneum embryogenesis depends on the proper control of glucose and glycogen.

  12. Genomics, transcriptomics, and peptidomics of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum

    DEFF Research Database (Denmark)

    Li, Bin; Predel, Reinhard; Neupert, Susanne; Hauser, Frank; Tanaka, Yoshiaki; Cazzamali, Giuseppe; Williamson, Michael; Arakane, Yasuyuki; Verleyen, Peter; Schoofs, Liliane; Schachtner, Joachim; Grimmelikhuijzen, Cornelis J P; Park, Yoonseong

    2008-01-01

    Neuropeptides and protein hormones are ancient molecules that mediate cell-to-cell communication. The whole genome sequence from the red flour beetle Tribolium castaneum, along with those from other insect species, provides an opportunity to study the evolution of the genes encoding neuropeptide...... for corazonin, kinin, and allatostatin-A. The cognate receptor genes for these three peptides also appear to be absent in the Tribolium genome. Our analysis of Tribolium indicates that, during insect evolution, genes for neuropeptides and protein hormones are often duplicated or lost....

  13. Estudo cefalométrico das alterações dentoesqueléticas da má oclusão de Classe II, divisão 1 tratada com o aparelho de Herbst com cantiléver Cephalometric study in patients that displayed Class II, division 1 malocclusion treated with herbst appliance

    Directory of Open Access Journals (Sweden)

    Ana Carla Raphaelli Nahás

    2008-02-01

    Full Text Available OBJETIVO: Realizou-se um estudo cefalométrico, em telerradiografias, objetivando-se determinar os efeitos no complexo craniofacial de pacientes com más oclusões de Classe II, divisão 1 submetidos ao tratamento com o aparelho de Herbst com cantiléver. METODOLOGIA: Para tanto, utilizou-se uma amostra composta por dois grupos, sendo um experimental e um controle. O grupo experimental originou-se da Faculdade de Odontologia de Bauru - USP, com 25 pacientes tratados com o aparelho ortopédico funcional e a idade inicial média de 12,01 anos. O grupo de controle, pareado cronologicamente ao grupo experimental, foi composto por pacientes não tratados ortodonticamente e/ou ortopedicamente, oriundos do arquivo de documentações denominado Burlington Growth Centre, localizado na Faculdade de Odontologia da Universidade de Toronto, Canadá. Para cada componente dos dois grupos, obtiveram-se as telerradiografias ao início (T1 e ao final (T2 do período de tratamento ou de observação, sendo traçadas manualmente e digitalizadas para um programa de cefalometria. Foram estabelecidas 33 grandezas cefalométricas. RESULTADOS E CONCLUSÕES: A comparação estatística entre o grupo experimental e o grupo controle (teste t de Student, com nível de significância pOBJECTIVE: This study investigated the treatment effects on the craniofacial growth of Class II patients treated with Herbst appliance. METHODOLOGY: The sample was comprised of two groups, one experimental and one control group. The experimental group originated from Bauru Dental School, University of São Paulo. This consisted of 25 patients treated by the Herbst appliance, with an initial mean age of 12,01 years. The control group, matched by age with the experimental group, was originated from the Burlington Growth Centre, located at the Faculty of Dentistry, University of Toronto, Canada. All patients were assessed at the beginning (T1 and at the end of the treatment or observation period

  14. Cap’n’collar differentiates the mandible from the maxilla in the beetle Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Coulcher Joshua F

    2012-11-01

    Full Text Available Abstract Background The biting mandible of the arthropods is thought to have evolved in the ancestor of the insects, crustaceans and myriapods: the Mandibulata. A unique origin suggests a common set of developmental genes will be required to pattern the mandible in different arthropods. To date we have functional studies on patterning of the mandibular segment of Drosophila melanogaster showing in particular the effects of the gene cap’n’collar (cnc, however, the dipteran head is far from representative of insects or of more distantly related mandibulates; Drosophila does not even possess a mandibular appendage. To study the development of a more representative insect mandible, we chose the red flour beetle Tribolium castaneum and investigated the function of the Tribolium orthologs of cap’n’collar (Tc-cnc and the Hox gene Deformed (Tc-Dfd. In order to determine the function of Tc-cnc and Tc-Dfd, transcripts were knocked down by maternal RNA interference (RNAi. The effects of gene knockdown were examined in the developing embryos and larvae. The effect of Tc-cnc and Tc-Dfd knockdown on the expression of other genes was determined by using in situ hybridization on Tribolium embryos. Results Our analyses show that Tc-cnc is required for specification of the identity of the mandibular segment of Tribolium and differentiates the mandible from maxillary identity. Loss of Tc-cnc function results in a transformation of the mandible to maxillary identity as well as deletion of the labrum. Tc-Dfd and the Tribolium homolog of proboscipedia (Tc-mxp = maxillopedia, Hox genes that are required to pattern the maxillary appendage, are expressed in a maxilla-like manner in the transformed mandible. Tribolium homologs of paired (Tc-prd and Distal-less (Tc-Dll that are expressed in the endites and telopodites of embryonic appendages are also expressed in a maxilla-like manner in the transformed mandible. We also show that Tc-Dfd is required to

  15. Molecular markers detect cryptic predation on coffee berry borer (Coleoptera: Curculionidae) by silvanid and laemophloeid flat bark beetles (Coleoptera: Silvanidae, Laemophloeidae) in coffee beans

    Science.gov (United States)

    The coffee berry borer, Hypothenemus hampei(Coleoptera: Curculionidae)(Ferrari), is a serious pest of coffee worldwide and has been recently introduced in Hawai’i, first detected in the state in 2010. Adult silvanid flat bark beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and adult laemoph...

  16. The cys-loop ligand-gated ion channel gene superfamily of the red flour beetle, Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Sattelle David B

    2007-09-01

    Full Text Available Abstract Background Members of the cys-loop ligand-gated ion channel (cys-loop LGIC superfamily mediate chemical neurotransmission and are studied extensively as potential targets of drugs used to treat neurological disorders such as Alzheimer's disease. Insect cys-loop LGICs are also of interest as they are targets of highly successful insecticides. The red flour beetle, Tribolium castaneum, is a major pest of stored agricultural products and is also an important model organism for studying development. Results As part of the T. castaneum genome sequencing effort, we have characterized the beetle cys-loop LGIC superfamily which is the third insect superfamily to be described after those of Drosophila melanogaster and Apis mellifera, and also the largest consisting of 24 genes. As with Drosophila and Apis, Tribolium possesses ion channels gated by acetylcholine, γ-amino butyric acid (GABA, glutamate and histamine as well as orthologs of the Drosophila pH-sensitive chloride channel subunit (pHCl, CG8916 and CG12344. Similar to Drosophila and Apis, Tribolium cys-loop LGIC diversity is broadened by alternative splicing although the beetle orthologs of RDL and GluCl possess more variants of exon 3. Also, RNA A-to-I editing was observed in two Tribolium nicotinic acetylcholine receptor subunits, Tcasα6 and Tcasβ1. Editing in Tcasα6 is evolutionarily conserved with D. melanogaster, A. mellifera and Heliothis virescens, whereas Tcasβ1 is edited at a site so far only observed in the beetle. Conclusion Our findings reveal that in diverse insect species the cys-loop LGIC superfamily has remained compact with only minor changes in gene numbers. However, alternative splicing, RNA editing and the presence of divergent subunits broadens the cys-loop LGIC proteome and generates species-specific receptor isoforms. These findings on Tribolium castaneum enhance our understanding of cys-loop LGIC functional genomics and provide a useful basis for the

  17. RNAi-mediated knockdown of the voltage gated sodium ion channel TcNav causes mortality in Tribolium castaneum

    Science.gov (United States)

    Abd El Halim, Hesham M.; Alshukri, Baida M. H.; Ahmad, Munawar S.; Nakasu, Erich Y. T.; Awwad, Mohammed H.; Salama, Elham M.; Gatehouse, Angharad M. R.; Edwards, Martin G.

    2016-01-01

    The voltage-gated sodium ion channel (VGSC) belongs to the largest superfamily of ion channels. Since VGSCs play key roles in physiological processes they are major targets for effective insecticides. RNA interference (RNAi) is widely used to analyse gene function, but recently, it has shown potential to contribute to novel strategies for selectively controlling agricultural insect pests. The current study evaluates the delivery of dsRNA targeted to the sodium ion channel paralytic A (TcNav) gene in Tribolium castaneum as a viable means of controlling this insect pest. Delivery of TcNav dsRNA caused severe developmental arrest with larval mortalities up to 73% post injection of dsRNA. Injected larvae showed significant (p insect control. PMID:27411529

  18. Translocation of bacteria from the gut to the eggs triggers maternal transgenerational immune priming in Tribolium castaneum.

    Science.gov (United States)

    Knorr, Eileen; Schmidtberg, Henrike; Arslan, Derya; Bingsohn, Linda; Vilcinskas, Andreas

    2015-12-01

    Invertebrates can be primed to enhance their protection against pathogens they have encountered before. This enhanced immunity can be passed maternally or paternally to the offspring and is known as transgenerational immune priming. We challenged larvae of the red flour beetle Tribolium castaneum by feeding them on diets supplemented with Escherichia coli, Micrococcus luteus or Pseudomonas entomophila, thus mimicking natural exposure to pathogens. The oral uptake of bacteria induced immunity-related genes in the offspring, but did not affect the methylation status of the egg DNA. However, we observed the translocation of bacteria or bacterial fragments from the gut to the developing eggs via the female reproductive system. Such translocating microbial elicitors are postulated to trigger bacterial strain-specific immune responses in the offspring and provide an alternative mechanistic explanation for maternal transgenerational immune priming in coleopteran insects. PMID:26701756

  19. Irradiation as an alternative treatment to methyl bromide for the disinfestation of tribolium castaneum in stored cacao

    International Nuclear Information System (INIS)

    Effects of irradiation on red flour beetle, Tribolium castaneum, eggs, larvae, pupae, and adults were examined. Data was taken on mortality, adult emergence, and sterility after irradiation at doses between 0.02 and 0.16 kGy. Mature eggs were more resistant to irradiation than young eggs; development to adults was prevented at a dose of 0.08 kGy for one to two day old eggs but was not stopped for the three to four day old eggs at doses as high as 0.16 kGy. Larvae were the most sensitive to irradiation. Young larvae appeared to be more resistant to irradiation than older larvae. Pupae were the most resistant to irradiation, and older pupae were more resistant than younger pupae. Development to adults was not prevented in both types of pupae up to 0.16 kGy. Survivors were not observed after four weeks for adults receiving a dose of 0.10 kGy or higher. Adults emerging from irradiated eggs, larvae, and pupae were sterile, in some cases, at slightly higher doses than those required to prevent adult emergence. Adults from irradiated eggs and larvae were sterile at 0.06 kGy. For pupae, sterility was achieved at 0.06 and 0.12 kGy for the one to two days and four to five days old pupae, respectively. Adult sterility was achieved at 0.12 kGy. As expected, when one member of a mating pair was left unirradiated, the dose requirements were higher. The effect of mating combination is still being analyzed. Data are still being collected and analyzed for Ephestia cautella, another major pest of stored cacao bean. Tests with T. castaneum and E. cautella must be completed before a dose can be recommended for commercial treatment. (author)

  20. A Defensin from the Model Beetle Tribolium castaneum Acts Synergistically with Telavancin and Daptomycin against Multidrug Resistant Staphylococcus aureus.

    Directory of Open Access Journals (Sweden)

    Rajmohan Rajamuthiah

    Full Text Available The red flour beetle Tribolium castaneum is a common insect pest and has been established as a model beetle to study insect development and immunity. This study demonstrates that defensin 1 from T. castaneum displays in vitro and in vivo antimicrobial activity against drug resistant Staphylococcus aureus strains. The minimum inhibitory concentration (MIC of defensin 1 against 11 reference and clinical staphylococcal isolates was between 16-64 μg/ml. The putative mode of action of the defensin peptide is disruption of the bacterial cell membrane. The antibacterial activity of defensin 1 was attenuated by salt concentrations of 1.56 mM and 25 mM for NaCl and CaCl2 respectively. Treatment of defensin 1 with the reducing agent dithiothreitol (DTT at concentrations 1.56 to 3.13 mM abolished the antimicrobial activity of the peptide. In the presence of subinhibitory concentrations of antibiotics that also target the bacterial cell envelope such as telavancin and daptomycin, the MIC of the peptide was as low as 1 μg/ml. Moreover, when tested against an S. aureus strain that was defective in D-alanylation of the cell wall, the MIC of the peptide was 0.5 μg/ml. Defensin 1 exhibited no toxicity against human erythrocytes even at 400 μg/ml. The in vivo activity of the peptide was validated in a Caenorhabditis elegans-MRSA liquid infection assay. These results suggest that defensin 1 behaves similarly to other cationic AMPs in its mode of action against S. aureus and that the activity of the peptide can be enhanced in combination with other antibiotics with similar modes of action or with compounds that have the ability to decrease D-alanylation of the bacterial cell wall.

  1. Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera)

    NARCIS (Netherlands)

    Alexander, K.

    2003-01-01

    Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera) Data are presented on the distribution of selected species from two coleopteran families chosen to represent a random slice of the British fauna. The species have been chosen as exhibiting extremes of range chang

  2. Noteworthy records of Hispines from Belize (Coleoptera: Chrysomelidae)

    OpenAIRE

    Naczi, Robert F. C.; Charles L. Staines

    2011-01-01

    Cephaloleia consanguinea Baly, Cephaloleia fulvolimbata Baly, Cephaloleia ruficollis Baly, Chalepus amabilis Baly, Chalepus brevicornis (Baly), Chalepus pici Descarpentries and Villiers, Microrhopala erebus (Newman), Octhispa bimaculata Uhmann, Octotoma championi Baly, Pseudispa tuberculata Staines, Sceloenopla erudita (Baly), Stenispa guatemalensis Uhmann, Sumitrosis gestroi (Weise), and Sumitrosis terminatus (Baly) (Coleoptera: Chrysomelidae: Cassidinae) are new country records of hispine c...

  3. A coffee berry borer (Coleoptera: Curculionidae: Scolytinae) bibliography

    Science.gov (United States)

    One hundred years ago, one of the most significant biological invasions of an agricultural insect pest in the Americas was initiated. Endemic to Africa, the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae) was accidentally introduced to Brazil in 1913 and years later invaded coffe...

  4. The Peritelini (Coleoptera: Curculionidae, Entiminae of the Vitale collection

    Directory of Open Access Journals (Sweden)

    Cosimo Baviera

    2016-01-01

    Full Text Available The collection of Peritelini (Coleoptera Curculionidae Entiminae currently stored in the Vitale collection of Messina University is an element of great importance for studies of taxonomy and biogeography of these rarely collected weevils. All species are commented in relation to the contributions to this taxonomic group, published on several occasions by the authors.

  5. Predation by Flat Bark Beetles (Coleoptera: Silvanidae and Laemophloeidae) on Coffee Berry Borer (Coleoptera: Curculionidae) in Hawaii coffee

    Science.gov (United States)

    Coffee berry borer(CBB), Hypothenemus hampei, is a serious pest of coffee worldwide and a new invasive pest in Hawaii. Adult flat bark beetles, mainly Leptophloeus sp.(75%) and Cathartus quadricollis(21%) (Coleoptera: Laemophloeidae and Silvanidae, respectively), were found feeding in CBB-infested c...

  6. “I don’t know a lot of rude words”: Herbst, Paretsky, and Grafton’s Struggle to Master the Tough Guy Voice

    Directory of Open Access Journals (Sweden)

    Laura Ng

    2013-09-01

    Full Text Available Abstract The proletarian literature of the 1930s and traditional 1930s era hard-boiled detective fiction are literary siblings. Both genres came of age in popular publications. Both genres deal with establishing order. Both genres use the “tough guy” voice. This voice was a no-nonsense powerful tool used to highlight the gritty realism of blue collar life. This style of writing quickly became a central component for both genres. Embedded in the term “tough guy voice” is masculine identity. This is the voice for the active underdog male hero. Writing in genres for women is an on-going challenge given the expectation of gender implied in the narrative voice. For the contemporary feminist hard-boiled detective and the 1930’s women proletarian protagonist to be taken seriously in their respective tough guy genres, they must show the same verbal acumen as the male characters who built the genres. At the core of each genre, the expectation that tough guys remain tough guys is an issue that stretches beyond the time frame of 1930s and 1940s to contemporary iterations of the genres. The gendered transition from male protagonist to female protagonist requires authors like Josephine Herbst, Sara Paretsky, and Sue Grafton to engage in a series of dynamic manipulations verbal and textual manipulations. Both sets of authors created protagonists that faced the threat of being dismissed due to their gender, traditional family roles, and conflicts with authority. The authors dealt with these threats using very similar strategies, to varying levels of success. Paretsky and Grafton’s heroines win verbal battles by showing mastery of the voice and the other voices in the texts.  At the core of this struggle is the protagonists’ ability to maintain the power of professing.

  7. Het voorkomen van de glanskevers van het genus Caprophilus in Nederland (Coleoptera: Nitidulidae)

    NARCIS (Netherlands)

    Oude, de J.

    2005-01-01

    The occurrence of nitidulid beetles of the genus Carpophilus in the Netherlands (Coleoptera: Nitidulidae) Some species of the genus Carpophilus (Coleoptera: Nitidulidae) may act as pests of stored products like cocoabeans, peanuts, dried fruits like figs and dates, imported from tropical or subtropi

  8. The model beetle Tribolium castaneum can be used as an early warning system for transgenerational epigenetic side effects caused by pharmaceuticals.

    Science.gov (United States)

    Bingsohn, Linda; Knorr, Eileen; Vilcinskas, Andreas

    2016-01-01

    Pharmaceuticals are not currently tested for transgenerational and epigenetic side effects. The use of vertebrates as preclinical research models is limited by their long generation times, low numbers of progeny and ethical concerns. In contrast, invertebrates such as insects breed rapidly, produce many offspring and are more ethically acceptable, allowing them to be used for high-throughput screening. Here, we established Tribolium castaneum as a model to screen for the effect of drugs on complex fitness parameters and the expression of epigenetic regulatory genes. We tested diets supplemented with the psychoactive drug valproic acid (VPA), which is a histone deacetylase inhibitor, or the antioxidant curcumin, which is a histone acetyltransferase inhibitor. We found that VPA delayed development, reduced longevity, and increased female body weight compared to a control diet. Fertility and fecundity declined and the expression of epigenetic regulatory genes was induced in the untreated F1 generation. In contrast, curcumin did not affect development or body weight, but it increased longevity, caused a significant reduction in fertility, and induced the expression of epigenetic regulatory genes mostly in the treated F0 generation. VPA and curcumin administered to vertebrate models have similar effects to those we observed in T. castaneum, confirming that this beetle is potentially useful as an alternative model to screen for the epigenetic effect of drugs. T. castaneum also provides a valuable early warning system for transgenerational epigenetic risk factors that are difficult to detect in mammals. PMID:26972758

  9. On the identity of European Baridini (Coleoptera, Curculionoidea) named before 1832

    Science.gov (United States)

    Twenty-eight names of European weevils usually regarded as Baridini in the literature are checked. Neotypes are designated for Curculio coerulescens Scopoli 1763 (emend.), C. glaber Herbst 1784, C. chalybeus Gmelin 1790, C. laticollis Marsham 1802, and C. picicornis Marsham 1802. Lectotypes are desi...

  10. Fumigant toxicity and oviposition deterrency of the essential oil from cardamom, Elettaria cardamomum, against three stored–product insects.

    Science.gov (United States)

    Abbasipour, Habib; Mahmoudvand, Mohammad; Rastegar, Fahimeh; Hosseinpour, Mohammad Hossein

    2011-01-01

    Use of insecticides can have disruptive effects on the environment. Replacing the chemical compounds in these insecticides with plant materials, however, can be a safe method with low environmental risk. In the current study, chemical composition and insecticidal activities of the essential oil from cardamom, Elettaria cardamomum L. (Maton) (Zingiberales: Zingiberaceae) on the adults of three stored product pests was investigated. Results indicated that essential oil of E. cardamomum toxic to the bruchid beetle, Callosobruchus maculatus Fabricius (Coleoptera: Bruchidae), the red flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae), and the flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Adults of E. kuehniella were more sensitive than the Coleoptera. Also, the highest mortality of these insects was seen after 12 hours. Results of the LT₅₀ tests showed that the lethal time of mortality occurred between 10-20 hours in various test concentrations. Essential oil of E. cardamomum had a good efficacy on oviposition deterrence of C. maculatus females, too. The chemical constituents of the essential oils were analyzed by gas chromatography-mass spectrometry. The major constituents of cardamom were identified as 1,8-cineol, α-terpinyl acetate, terpinene and fenchyl alcohol. These results suggest that essential oil of E. cardamomum is a good choice for control of stored product pests. PMID:22242564

  11. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, Jessica; Verheggen, François; Lognay, Georges; Haubruge, Eric

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and...

  12. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, J.; Verheggen, F.; Lognay, G.; Haubruge, E.

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and ...

  13. A Soluble Pyrophosphatase Is Essential to Oogenesis and Is Required for Polyphosphate Metabolism in the Red Flour Beetle (Tribolium castaneum

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    Klébea Carvalho

    2015-03-01

    Full Text Available Polyphosphates have been found in all cell types examined to date and play diverse roles depending on the cell type. In eukaryotic organisms, polyphosphates have been mainly investigated in mammalian cells with few studies on insects. Some studies have demonstrated that a pyrophosphatase regulates polyphosphate metabolism, and most of them were performed on trypanosomatids. Here, we investigated the effects of sPPase gene knocked down in oogenesis and polyphosphate metabolism in the red flour beetle (Tribolium castaneum. A single sPPase gene was identified in insect genome and is maternally provided at the mRNA level and not restricted to any embryonic or extraembryonic region during embryogenesis. After injection of Tc-sPPase dsRNA, female survival was reduced to 15% of the control (dsNeo RNA, and egg laying was completely impaired. The morphological analysis by nuclear DAPI staining of the ovarioles in Tc-sPPase dsRNA-injected females showed that the ovariole number is diminished, degenerated oocytes can be observed, and germarium is reduced. The polyphosphate level was increased in cytoplasmic and nuclear fractions in Tc-sPPase RNAi; Concomitantly, the exopolyphosphatase activity decreased in both fractions. Altogether, these data suggest a role for sPPase in the regulation on polyphosphate metabolism in insects and provide evidence that Tc-sPPase is essential to oogenesis.

  14. Use of Gamma Irradiation for the Control of the Red Flour Beetle (Tribolium castaneum Hrbst) in Bread Flour

    International Nuclear Information System (INIS)

    Three doses of gamma irradiation (viz. 2, 2.5 and 3 KGy) were tested against the red flour beetle (Tribolium castaneum Hrbst) in bread flour in Khartoum, Sudan. Corresponding control exhibited two groups of infested and uninfested flours. The results obtained showed that all the doses used reduced the infestation in a range 46-100 % during a six month storage period. The 2 KGy mortality range, of the test insect, was 46 - 100%, whereas the corresponding readings for 2.5 KGy and 3 KGy were 90 - 100% and total kill (100%), respectively. However, a natural infestation occurred in all the treated flours and in the infested and uninfested controls as well. This may refer to the resistant eggs of this insect whose a smaller size than the major bulk flour particles and therefore not affected by the milling process and the irradiation doses used too.The treatment mortality in this test was corrected by the Abbott's formula. Moreover, the flour analyses results implied that all the chemical parameters (moisture %, ash % and protein %) and the quality parameters (wet gluten and falling number) are within the recommended levels of the Codex Alimentarius.It was also found that these doses used had no harmful effect on dough rheological properties.

  15. Experimental removal of sexual selection leads to decreased investment in an immune component in female Tribolium castaneum.

    Science.gov (United States)

    Hangartner, Sandra; Michalczyk, Łukasz; Gage, Matthew J G; Martin, Oliver Y

    2015-07-01

    Because of divergent selection acting on males and females arising from different life-history strategies, polyandry can be expected to promote sexual dimorphism of investment into immune function. In previous work we have established the existence of such divergence within populations where males and females are exposed to varying degrees of polyandry. We here test whether the removal of sexual selection via enforced monogamy generates males and females that have similar levels of investment in immune function. To test this prediction experimentally, we measured differences between the sexes in a key immune measurement (phenoloxidase (PO) activity) and resistance to the microsporidian Paranosema whitei in Tribolium castaneum lines that evolved under monogamous (sexual selection absent) vs polyandrous (sexual selection present) mating systems. At generation 49, all selected lines were simultaneously assessed for PO activity and resistance to their natural parasite P. whitei after two generations of relaxed selection. We found that the polyandrous regime was associated with a clear dimorphism in immune function: females had significantly higher PO activities than males in these lines. In contrast, there was no such difference between the sexes in the lines evolving under the monogamous regime. Survival in the infection experiment did not differ between mating systems or sexes. Removing sexual selection via enforced monogamy thus seems to erase intersexual differences in immunity investment. We suggest that higher PO activities in females that have evolved under sexual selection might be driven by the increased risk of infections and/or injuries associated with exposure to multiple males. PMID:25958137

  16. 3D standard brain of the red flour beetle Tribolium castaneum: a tool to study metamorphic development and adult plasticity

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    David Dreyer

    2010-03-01

    Full Text Available The red flour beetle Tribolium castaneum is emerging as a further standard insect model beside Drosophila. Its genome is fully sequenced and it is susceptible for genetic manipulations including RNA-interference. We use this beetle to study adult brain development and plasticity primarily with respect to the olfactory system. In the current study, we provide 3D standard brain atlases of freshly eclosed adult female and male beetles (A0. The atlases include eight paired and three unpaired neuropils including antennal lobes, optic lobe neuropils, mushroom body calyces and pedunculi, and central complex. For each of the two standard brains, we averaged brain areas of 20 individual brains. Additionally, we characterized eight selected olfactory glomeruli from 10 A0 female and male beetles respectively, which we could unequivocally recognize from individual to individual owing to their size and typical position in the antennal lobes. In summary, comparison of the averaged neuropil volumes revealed no sexual dimorphism in any of the reconstructed neuropils in A0 Tribolium brains. Both, the female and male 3D standard brain are also used for interspecies comparisons, and, very importantly, will serve as future volumetric references after genetical manipulation especially regarding metamorphic development and adult plasticity.

  17. A Tribolium castaneum whole-embryo culture protocol for studying the molecular mechanisms and morphogenetic movements involved in insect development.

    Science.gov (United States)

    Macaya, Constanza C; Saavedra, Patricio E; Cepeda, Rodrigo E; Nuñez, Viviana A; Sarrazin, Andres F

    2016-01-01

    The development of the red flour beetle Tribolium castaneum is more representative of arthropods than the evolutionarily derived fly, Drosophila melanogaster. Thus, Tribolium is becoming an emerging organism model for studying the evolution of the mechanisms that control embryonic development in arthropods. In this regard, diverse genetic and molecular tools are currently available for Tribolium, as well as imaging and embryonic techniques. Recently, we developed a method for culturing embryos in order to study specific stages during Tribolium development. In this report, we present a detailed and "easy-to-follow" protocol for embryo handling and dissection, extending the use of whole-embryo culture to functional analysis by performing in vivo pharmacological manipulations. This experimental accessibility allowed us to study the relevance of microtubules in axis elongation, using nocodazole and taxol drugs to interfere with microtubule networks, followed by length measurement analysis. Additionally, we demonstrated that embryo handling had no effect on the development of Tribolium embryos, and we checked viability after dissection and bisection and during incubation using propidium iodide. The embryo culture protocol we describe here can be applied to study diverse developmental processes in Tribolium. We expect that this protocol can be adapted and applied to other arthropods. PMID:26739999

  18. Nocturnal Migration of Coleoptera: Carabidae in North China

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Vertical-looking radar (VLR) has allowed long-term automatic monitoring of the altitudinal and temporal dynamics of high-flying insect populations. To investigate whether ground beetle, insect of Coleoptera, was capable of migrating and its migration pattern by taking advantages of capability of the VLR for long-term real-time automatic monitoring, the migration of Coleoptera ground beetle was investigated by setting up radar observation points, making long-term observation using the VLR and related supplementary equipment, and analyzing low altitude air current and large area circulating current in combination with the meteorological data. Information obtained in 2005 and 2006 showed that the seasonal activities of ground beetles traps of trap lamps were mainly from late June to late August, peak period was mainly in August, seasonal traps of high-altitude lamps and ground lamps were featured by sudden increase and sudden decrease; in peak period, the height of radar echo point could be as high as 600 m, while it was mainly below the height of 450 m; night activities mainly occurred from 20:00 to 22:00, in very few nights, radar echo could last until about 04:00, changes in numbers of ground beetles within the searchlights were consistent with radar echo intensity; ground beetle images were successfully trapped in the sweep nets carried by captive balloons at the height of 200 m. Some species of Carabidaes had some degrees of migration, thus providing the foundation for investigating the migration of Coleoptera insects.

  19. Estados inmaduros de Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini Immature stages of Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini

    Directory of Open Access Journals (Sweden)

    Jhon César Neita-Moreno

    2008-12-01

    Full Text Available Se describen y se ilustran por primera vez la larva de tercer estadio y la pupa de Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini. Se aportan datos sobre la biología de la especie y su distribución en Colombia. Se proporciona una clave para la identificación de las larvas de tercer estadio conocidas de las especies del género Ancognatha Erichson.The third instar larva and pupa of Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini are described and illustrated for the first time. New data on larval biology and distribution of the species in Colombia are included. A key to the known third stage larvae of Ancognatha Erichson is provided.

  20. Laboratory Bioassay of Iranian Isolates of Entomopathogenic Fungus Metarhizium anisopliae (Metsch. Sorokin (Ascomycota: Hypocreales against two Species of Storage Pest

    Directory of Open Access Journals (Sweden)

    Hamid Sakenin Chelav

    2013-03-01

    Full Text Available The susceptibility of adults of Tribolium castaneum Herbst (Coleoptera: Tenebrionidae and Oryzaephilus surinamensis L. (Coleoptera: Silvanidae to three Iranian Isolates of the entomopathogenic fungi Metarhizium anisopliae (Metsch. Sorokin was evaluated through bioassays with direct immersion under laboratory conditions. For each isolates, five aqueous suspensions were prepared in a logarithmic series in Tween 80 (0.05% v/v. Results showed that adult of both species were susceptible to all isolates of M. anisopliae. For all three isolates, mortality percentage of the two species increased with increasing conidial concentration and significant difference was observed between concentrations. The corrected cumulative mortality of adult of O. surinamensis 10 days after immersion ranged from 12.38 to 85.84%, 18.6 to 62.83% and 10.63 to 77.87% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. These amounts for T. castaneum varied from 31.07 to 74.78%, 26.02 to 75.61% and 23.33 to 89.99% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. The parameters of probit analysis demonstrated non-overlap of 95% confidence limits of LC50 and LC95 and significant difference was observed among three isolates tested against each insect. The lowest and the highest LC50 and LC95 values were observed in the isolates DEMI001 for O. surinamensis (3/1×105 and 1/5×108 and IRAN 715C for T. castaneum (6.2×108 and 6.9×1014, respectively. This observation highlights the importance and need of screening for more virulent isolates against storage pests for use in the management of these pests.

  1. Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum

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    Xiao Liang

    2015-01-01

    Full Text Available Some cytochrome P450 (CYP genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively, permethrin (2.00- and 2.03-fold and lambda-cyhalothrin (1.73- and 1.81-fold, whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

  2. Primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae and Disteniidae) of the Smithsonian Institution

    Science.gov (United States)

    The primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae, Disteniidae) of the National Museum of Natural History (Smithsonian Institution) are catalogued and figured, current through 2012 (but also including some 2013 holotypes). Data on the original combination, current combina...

  3. New records of predaceous diving beetles (Coleoptera:Dytiscidae) in Maine

    Science.gov (United States)

    Boobar, L.R.; Gibbs, K.E.; Longcore, J.R.; Perillo, A.M.

    1996-01-01

    Locations, habitat descriptions, and collection dates are listed for new records of 4 genera and 12 species of predaceous diving beetles (Coleoptera: Dytiscidae) in Maine. Previously, 17 genera and 53 species of the aquatic beetle were reported from Maine.

  4. First record of Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae in Brazil

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2015-03-01

    Full Text Available Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae is recorded for the first time in Brazil (Bahia. It was originally described from Europe and is currently widely distributed in that continent and Asia.

  5. Methods for assessing infestations of sunflower stem weevil (Coleoptera: Curculionidae) in sunflower stems

    Science.gov (United States)

    The sunflower stem weevil, Cylindrocopturus adspersus LeConte (Coleoptera: Curculionidae), reduces sunflower, Helianthus annuus L. (Asteraceae), yields by spreading pathogens, damaging vascular tissues, and promoting lodging of sunflower plants. To assess weevil populations for host plant resistanc...

  6. A new species of the genus Falsoibidion Pic (Coleoptera, Cerambycidae) from Korea

    OpenAIRE

    Lee, Seunghyun; Lee, Seunghwan

    2016-01-01

    A new species of the genus Falsoibidion Pic, 1922 (Coleoptera, Cerambycidae, Cerambycinae, Callidiopini) from Korea is described. Habitus and genitalia of male and female of the new species are illustrated.

  7. Biologi Hama Kumbang Penggerek Pucuk Kelapa Sawit (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) Di Rumah Kassa

    OpenAIRE

    Sejahtra, Ahmad

    2011-01-01

    Ahmad Sejahtra, "BIOLOGY SHOOT BORERS BEETLE PEST OF COCONUT PALM (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) AT KASSA HOUSE", Under supervised by Ms Marheni and Ms Fatima Zahara. This study aims to determine biological pest of oil palm bud weevil (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) at home kassa. The experiment was conducted on people's plantations in PERUMNAS Simalingkar starting from September 2010 until April 2011. Research using observational methods for this resear...

  8. Papel dos besouros (Insecta, Coleoptera) na Entomologia Forense

    OpenAIRE

    Wellington Emanuel dos Santos

    2014-01-01

    Esse trabalho apresenta uma revisão do papel que os besouros (Insecta, Coleoptera) desempenham na Entomologia Forense. Discussões sobre ocorrência em cadáveres humanos e carcaças animais, estimativas de Intervalo Pós-Morte (IPM), estudos realizados no Brasil e em outros países, principais famílias de importância forense e aspectos biológicos, ecológicos e biogeográficos das espécies são apresentadas.

  9. Fossil history of Mesozoic weevils (Coleoptera:Curculionoidea)

    Institute of Scientific and Technical Information of China (English)

    Andrei A.Legalov

    2012-01-01

    The first synopsis of Mesozoic weevils (Curculionoidea: Coleoptera) is presented.Changes of family,genera and species abundance during the Mesozoic revealed three distributional patterns.The Jurassic (Karatau) fauna was dominated by the Nemonychidae.During the Early Cretaceous (beginning at the Jurassic/Cretaceous border),the Ithyceridae was the prevalent group with a significant role played by the Nemonychidae.In the Late Cretaceous (Cenomanian and Turonian),the major groups were the Curculionidae and Brentidae.Obviously,the change of weevil fauna during this period was due to the expansion of the angiosperms,which provided multiple niches in their vegetative and reproductive organs for weevil development.

  10. An annotated catalogue of the Buprestidae of Iran (Coleoptera: Buprestoidea).

    Science.gov (United States)

    Ghahari, Hassan; Volkovitsh, Mark G; Bellamy, Charles L

    2015-01-01

    An annotated taxonomic catalogue of the jewel beetles (Coleoptera: Buprestidae) of Iran is given. Original descriptions and recent revisionary or catalogue data are included along with the distribution, both within and outside of Iran, ecological data and host plant associations, junior synonyms, and comments. A complete bibliography completes the catalogue. In total 428 species and 52 subspecies of jewel beetles belonging to 6 subfamilies (Julodinae, Polycestinae, Galbellinae, Chrysochroinae, Buprestinae, and Agrilinae), 20 tribes, and 38 genera are known from Iran including doubtful records and 4 nomina nuda. It is likely that the number of jewel beetle species from Iran will be between 460-480 and possibly even more species. PMID:26250020

  11. SCARABAEUS SEROTINUS PANZER, 1799 (CURRENTLY NOBIUS SEROTINUS) A VALID NAME (NOMEN PROTECTUM) (COLEOPTERA, APHODIIDAE)

    OpenAIRE

    Tristão Branco; Giovanni Dellacasa; Marco Dellacasa

    2008-01-01

    Il nome Scarabaeus serotinus Panzer, 1799 (attualmente Nobius serotinus), ad oggi usato correntemente come nome valido, risulta sinonimo juniore di Scarabaeus minu­tus Herbst, 1783. Vengono dunque applicate le norme previste dall’articolo 23.9 del Codice Internazionale per la Nomenclatura Zoologica al fine di ottenere un’inversione di priorità a vantaggio del nome più recente (considerato nomen protectum) rispetto al nome più antico (considerato nomen oblitum). Si stabilisce inoltre la pri...

  12. Genomic and gene regulatory signatures of cryptozoic adaptation: Loss of blue sensitive photoreceptors through expansion of long wavelength-opsin expression in the red flour beetle Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Cook Tiffany A

    2007-12-01

    Full Text Available Abstract Background Recent genome sequence analysis in the red flour beetle Tribolium castaneum indicated that this highly crepuscular animal encodes only two single opsin paralogs: a UV-opsin and a long wavelength (LW-opsin; however, these animals do not encode a blue (B-opsin as most other insects. Here, we studied the spatial regulation of the Tribolium single LW- and UV-opsin gene paralogs in comparison to that of the five opsin paralogs in the retina of Drosophila melanogaster. Results In situ hybridization analysis reveals that the Tribolium retina, in contrast with other insect retinas, constitutes a homogenous field of ommatidia that have seven LW-opsin expressing photoreceptors and one UV-/LW-opsin co-expressing photoreceptor per eye unit. This pattern is consistent with the loss of photoreceptors sensitive to blue wavelengths. It also identifies Tribolium as the first example of a species in insects that co-expresses two different opsins across the entire retina in violation of the widely observed "one receptor rule" of sensory cells. Conclusion Broader studies of opsin evolution in darkling beetles and other coleopteran groups have the potential to pinpoint the permissive and adaptive forces that played a role in the evolution of vision in Tribolium castaneum.

  13. Identification and characterization of the Sudanese Bacillus thuringiensis and related bacterial strains for their efficacy against Helicoverpa armigera and Tribolium castaneum.

    Science.gov (United States)

    Gorashi, N E; Tripathi, M; Kalia, V; Gujar, G T

    2014-06-01

    Forty-four isolates of Bacillus thuringiensis like bacteria from various sources in different locations from Sudan were tested for their insecticidal activity. The toxicity of these isolates ranged from 6.6 to 70% to the neonates of cotton bollworm, Helicoverpa armigera at 10 ppm concentration. The most effective ones are Kb-29, St-6 and Wh-1 comparable with HD-1. Toxicity of isolates to larvae of the red flour beetle, Tribolium castaneum ranged from 20 to 100%. Isolates St-2 and St-23 gave 100% larval mortality within 15 days of exposure and were at par with Ab-8, Ab-12, Kb-26, Kb-30, Om-4, Po-2, Po-5, Po-7, Sa-8 and Wh-5 and were also comparable with E. coli clone expressing Cry3 toxin. The most effective five isolates viz., Kb-29, St-2, St-6, St-23 and Wh-1 belonged to B. thuringiensis. The St-6 isolate, which also showed high toxicity to T. castaneum larvae, had cry1 genes along with coleopteran active cry28 genes, but not cry3 genes. Of the 25 isolates characterized with 16s DNA sequencing, seven belonged to Paenibacillus spp., one Lysinibacillus sphaericus, one Bacillus pumilus, four Bacillus spp., and rest 12 belonged to B. thuringiensis. Biochemical characterization in each species showed variation. The present study shows potential of some isolates like Kb-29, St-2, St-6, St-23 and Wh-1 as promising bioinsecticides. PMID:24956895

  14. The Effect of Temperature and Humidity on the Susceptibility of Flour Beetles to Organophosphorus Insecticides

    Directory of Open Access Journals (Sweden)

    D. B. Chadha

    1965-01-01

    Full Text Available The effect of the temperature and humidity on the susceptibility of Tribolium castaneum Herbst. to malathion and diazinon, has been investigated. It has been found that temperature significantly influences the susceptibility of T. castaneum to malathion though not to diazinon. Humidity, however, influences the susceptibility if the insect to both insecticides.

  15. The complete mitochondrial genome of Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae).

    Science.gov (United States)

    Sun, Tian Yi; Li, Lei; Xin, Tianrong; Wang, Yayu; Xia, Bin

    2016-09-01

    We determined the complete mitochondrial genome (mitogenome) sequence of Cryptolestes ferrugineus (GenBank accession number KT182067) by the long PCR and primer walking method. The mitochondrial genome is a typical circular DNA molecule of 15 511 bp in length, and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region (D-loop). The order of 37 genes was typical of insect mitochondrial DNA sequences described to date. The base composition of the genome is A (39.17%), T (37.24%), C (14.22%), and G (9.37%) with an A + T-rich hallmark as that of other invertebrate mitochondrial genomes. All protein-coding genes start with ATN codon and terminate with the stop codon T (AA) or TAG. The A + T-rich region is located between 12S rRNA and tRNA(Ile). In this study, the phylogenetic relationships of Coleoptera species were constructed based on the nucleotides sequences of 13 PCGs of mitogenomes. The molecular-based phylogeny supported the traditional morphological classification on relationships within Coleoptera species. PMID:26330111

  16. Rove beetles of medical importance in Brazil (Coleoptera, Staphylinidae, Paederinae

    Directory of Open Access Journals (Sweden)

    Juliana S. Vieira

    2014-09-01

    Full Text Available Rove beetles of medical importance in Brazil (Coleoptera, Staphylinidae, Paederinae. The rove beetles of the genus Paederus Fabricius, 1775 are the most important group within Coleoptera causing dermatitis around the world. The medical importance of Paederus depends on its toxic hemolymph released when these beetles are crushed on human skin. The effects are mainly dermatitis linearis and some sporadic cases of conjunctivitis. In Brazil seven species of Paederus are known to cause dermatitis: P. amazonicus Sharp, 1876, P. brasiliensis Erichson, 1840, P. columbinus Laporte, 1835, P. ferus Erichson, 1840, P. mutans Sharp, 1876, P. protensus Sharp, 1876 stat. rev., and Paederus rutilicornis Erichson, 1840. Paederus mutans and P. protensus are for the first time recorded as of medical importance, whereas the record of P. rutilicornis in Brazil is doubtful. All seven species are redescribed and a dichotomous key is provided. The geographic distributions of all species are documented. The results provided here include the most recent and relevant taxonomic revision of Paederus of the Neotropical region, the first identification key for Brazilian species and the increase of recorded species of medical importance in the world.

  17. Complete mitochondrial genome of Cryptolestes pusillus (Coleoptera: Laemophloeidae).

    Science.gov (United States)

    Li, Lei; Liu, Guanghua; Sun, Tanyi; Xin, Tianrong; Li, Meiyun; Zou, Zhiwen; Xia, Bin

    2016-09-01

    In this study, the complete mitochondrial genome of Cryptolestes pusillus (GenBank accession number KT070713) was sequenced by long PCR and primer walking methods. The total length of mitochondrial DNA is 15 502 bp and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region. The base composition of the genome is A (39.04%), T (37.07%), C (23.4%), and G (14.6%). Except for COI and ATP8 with TCC and ATC as start codon, respectively, the remaining protein-coding genes initiated with the three orthodox start codons. Two complete stop codons (TAA and TAG) and two incomplete stop codons (COIII stop with T and ND5 stop with TA) were used in the protein-coding genes. The A + T-rich region is located between 12s rRNA and tRNA(Ile) with the length of 859 bp. The phylogenetic relationships of Coleoptera species were constructed based on the nucleotide sequences of 13 protein-coding genes of mitogenome using the neighbor-joining method. The molecular-based phylogenetic analysis supported the traditional morphological classification on relationships within Coleoptera species. PMID:26329895

  18. ON SEVEN OVERLOOKED CURCULIONOIDEA FROM NORTHERN ITALY (Coleoptera

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    Roberto Caldara

    2012-04-01

    Full Text Available Seven Curculionoidea described from Lombardy (northern Italy by Virginio Betta in 1857 and overlooked by subsequent authors are here treated. Since the Betta collection where their types are supposed to have been preserved was never traced, neotypes of all of them are designated with the purpose of clarifying their taxonomic status. Revised combinations are: Pseudeuparius pictus (Betta, 1857 comb. rev. from Tropideres Schoenherr, 1823; Pachytychius aericollis (Betta, 1857 comb. rev. from Smicronyx Schoenherr, 1843; Mecinus vittatus (Betta, 1857 comb. rev. from Gymnetron Schoenherr, 1825; Mecinus areolatus (Betta, 1857 comb. rev. from Gymnetron Schoenherr; Mecinus flavipes (Betta, 1857 comb. rev. from Gymnetron Schoenherr. The following new synonymies are established: Pseudeuparius centromaculatus (Gyllenhal, 1833 [= P. pictus (Betta, 1857 syn. n.]; Pachytychius sparsutus (Olivier, 1807 [= P. aericollis (Betta, 1857 syn. n.]; Smicronyx coecus (Reich, 1797 [= S. minusculus (Betta, 1857 syn. n.]; Mecinus labilis (Herbst, 1795 [= M. vittatus (Betta, 1857 syn. n.]; Mecinus pascuorum (Gyllenhal, 1813 [= M. areolatus (Betta, 1857 syn. n.]; Mecinus latiusculus (Jacquelin du Val, 1855 [= M. flavipes (Betta, 1857 syn. n.]; Mecinus circulatus (Marsham, 1802 [= M. sericatus Betta, 1857 syn. n.].

  19. Chaves para a identificação dos principais Coleoptera (Insecta) associados com produtos armazenados Keys for the identification of Coleoptera (Insecta) associated with stored products

    OpenAIRE

    Paulo Roberto Valle da Silva Pereira; Lúcia Massutti de Almeida

    2001-01-01

    An illustrated key to identify nine families of Coleoptera commonly found in stored products is presented. Keys for the identification of Anobiidae [Lasioderma serricorne (Fabricius, 1792), Stegobium paniceum (Linnaeus, 1761)], Bruchidae [Acanthoscelides obtectus (Say, 1831), Zabrotes subfasciatus (Boheman, 1833)], Curculionidae [Sitophilus oryzae (Linnaeus, 1763), S. zeamais Motschulsky, 1885], Silvanidae [Ahasverus advena (Waltl, 1832), Cathartus quadricollis (Guérin, 1892), Oryzaephilus me...

  20. Biology and Management of Billbugs (Coleoptera: Curculionidae) in Turfgrass

    Science.gov (United States)

    Dupuy, Madeleine M.; Ramirez, Ricardo A.

    2016-01-01

    Billbugs (Coleoptera: Curculionidae: Sphenophorus spp.) are a complex of weevil pests affecting turfgrass throughout the United States. Billbug larvae cause damage by feeding in stems, on roots, and on the crowns of turf, causing severe discoloration and eventual plant death. Monitoring efforts have focused on nondestructive pitfall sampling of ground-active billbug adults and on destructive sampling using soil cores for larval stages in the soil. Given the cryptic nature of the susceptible larval stages, billbugs are typically managed by preventive applications of long-residual, systemic insecticides, including neonicotinoids and anthranilic diamides. Despite knowledge of effective management practices including pest-resistant turf varieties, irrigation management, and microbial controls that contribute to an IPM approach, billbug management continues to rely heavily on prophylactic synthetic insecticides. This review will summarize the identification and biology of billbugs and strategies for their management. PMID:27065080

  1. BESOUROS COPRÓFAGOS (COLEOPTERA; SCARABAEIDAE COLETADOS EM PIRACICABA, SP

    Directory of Open Access Journals (Sweden)

    RODRIGUES S.R.

    1998-01-01

    Full Text Available Através do uso de armadilhas "pitfall" iscadas com massa fecal fresca de bovinos, realizou-se a coleta de besouros coprófagos (Coleoptera; Scarabaeidae, durante o período de 15 de abril de 1995 a 17 de fevereiro de 1996, em área de pastagem ao lado de confinamento de bovinos, em Piracicaba, SP. Coletou-se um total de 11 espécies distribuidas nos gêneros Aphodius, Ataenius, Trichillum, Eurysternus, Dichotomius e Canthon. Os besouros coprófagos de comportamento endocoprídeo representaram 72,73% das espécies coletadas, sendo estes os de maior ocorrência e a espécie Aphodius lividus parece ser a mais bem adaptada na área estudada.

  2. Track analysis of the Neotropical Entimini (Coleoptera: Curculionidae: Entiminae

    Directory of Open Access Journals (Sweden)

    Alberto Romo

    2011-09-01

    Full Text Available Track analysis of the Neotropical Entimini (Coleoptera, Curculionidae, Entiminae. Distributional patterns of the species belonging to the tribe Entimini from the Neotropical region were analyzed. Based on a track analysis of 22 species of Entimus, Rhigus, and Phaedropus, for which distributional data were available, two generalized tracks were found. One is located in northern Brazil, corresponding to the Amazonian subregion, and is determined by Phaedropus candidus and Rhigus speciosus. The other is located in southern Brazil, corresponding to the Parana subregion, and is determined by Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus, and R. tribuloides. The development of the Chacoan subregion is hypothesized to have been the dynamic vicariant event that fragmented the former Amazonian-Parana forest.Análisis de trazos de Entimini Neotropical (Coleoptera, Curculionidae, Entiminae. Se analizaron los patrones de distribución de la tribu Entimini en la región Neotropical. Con base en un análisis de los trazos de 22 especies de Entimus, Rhigus y Phaedropus, para los cuales se contaba con datos de distribución, se hallaron dos trazos generalizados. Uno se localiza en el norte de Brasil, en la subregión Amazónica, y está sustentado por Phaedropus candidus y Rhigus speciosus. El otro se localiza en el sur de Brasil, en la subregión Paranaense, y está sustentado por Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus y R. tribuloides. Se hipotetiza que el desarrollo de la subregión Chaqueña constituyó el evento de vicarianza dinámica que fragmentó el antiguo bosque Amazónico-Paranaense.

  3. New Curculionoidea (Coleoptera records for Canadа

    Directory of Open Access Journals (Sweden)

    Hume Douglas

    2013-06-01

    schevyrewi Semenov Tjan-Shansky, 1902; Tyloderma foveolatum (Say, 1832; (all Curculionidae; Ontario – Trichapion nigrum (Herbst, 1797; Nanophyes marmoratus marmoratus (Goeze, 1777 (both Brentidae; Asperosoma echinatum (Fall, 1917; Micracis suturalis LeConte, 1868; Orchestes alni (Linnaeus, 1758; Phloeosinus pini Swaine, 1915; Scolytus schevyrewi Semenov Tjan-Shansky, 1902; Xyleborinus attenuatus (Blandford, 1894 (all Curculionidae; Quebec – Trigonorhinus alternatus (Say, 1826; Trigonorhinus tomentosus tomentosus (Say, 1826 (both Anthribidae; Trichapion nigrum (Herbst, 1797; Trichapion porcatum (Boheman, 1839; Nanophyes marmoratus marmoratus (Goeze, 1777 (all Brentidae; Lissorhoptrus oryzophilus Kuschel, 1952 (Brachyceridae; Acalles carinatus LeConte, 1876; Ampeloglypter ampelopsis (Riley, 1869; Anthonomus rufipes LeConte, 1876; Anthonomus suturalis LeConte, 1824; Ceutorhynchus hamiltoni Dietz, 1896; Curculio pardalis (Chittenden, 1908; Cyrtepistomus castaneus (Roelofs, 1873; Larinus planus (Fabricius, 1792; Mecinus janthinus (Germar, 1821; Microhyus setiger LeConte, 1876; Microplontus campestris (Gyllenhal, 1837; Orchestes alni (Linnaeus, 1758; Otiorhynchus ligustici (Linnaeus, 1758; Rhinusa neta (Germar, 1821; Trichobaris trinotata (Say, 1832; Tychius liljebladi Blatchley, 1916; Xyleborinus attenuatus (Blandford, 1894; Xyleborus affinis Eichhoff, 1868 (all Curculionidae; Sphenophorus incongruus Chittenden, 1905 (Dryophthoridae; New Brunswick – Euparius paganus Gyllenhal, 1833; Allandrus populi Pierce, 1930; Gonotropis dorsalis (Thunberg, 1796; Euxenus punctatus LeConte, 1876 (all Anthribidae; Loborhynchapion cyanitinctum (Fall, 1927 (Brentidae; Pseudanthonomus seriesetosus Dietz, 1891; Curculio sulcatulus (Casey, 1897; Lignyodes bischoffi (Blatchley, 1916; Lignyodes horridulus (Casey, 1892; Dietzella zimmermanni (Gyllenhal, 1837; Parenthis vestitus Dietz, 1896; Pelenomus squamosus LeConte, 1876; Psomus armatus Dietz, 1891; Rhyncolus macrops Buchanan, 1946; Magdalis

  4. Bio-edafology of the Coleoptera order, in three Colombia Natural Regions

    International Nuclear Information System (INIS)

    The characterization of Coleoptera families is showed to three different Colombian Natural Regions. This is given in relation with biological and environmental factors, different vegetation covers and soil uses. In addition to it, susceptible Coleoptera taxa are determined when natural conditions are disturbed. Methodically, a literature subject research was made, and Barber and Berlesse traps were used to organism extractions from superficial and under superficial soil. Horizons diversity, riches and constancy index were determined to each family. Results show different diversity, riches and constancy values to each family, as much in each natural region, such as to each soil use

  5. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae)

    OpenAIRE

    Dilma Solange Napp; Ubirajara Ribeiro Martins

    2006-01-01

    Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae). Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae). The following new genus and species, all from Bolivia, are describ...

  6. Influence of growing location and cultivar on Rhyzopertha dominica (Coleoptera: Bostrichidae) and Sitophilus oryzae (Coleoptera: Curculionidae) infestation of rough rice

    Institute of Scientific and Technical Information of China (English)

    FRANK H. ARTHUR; RUSTY C. BAUTISTA; TERRENCE J. SIEBENMORGEN

    2007-01-01

    Long-grain rice cultivars Cocodrie, Wells, and XP 723 grown in three locations (Hazen, MO; Essex and Newport, AR, USA), and medium-grain rice cultivars Bengal and XP 713 grown in two locations (Jonesboro and Lodge Corner, AR, USA), were harvested and assayed for susceptibility to Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), the lesser grain borer, and Sitophilus oryzae (L.) (Coleoptera: Curculionidae), the rice weevil, on rice held at 27℃ 57% and 75% relative humidity (RH).Separate samples from the same harvest lots were also analyzed for the physical characteristics of brown rice yield, percentage whole kernels and kernel thickness. Progeny production and feeding damage of R. dominica were significantly different among long-grain cultivars within two of the three locations (P < 0.05), but not for location or RH (P ≥ 0.05), while progeny production of S. oryzae was different among cultivars, location, and RH (P < 0.05). On medium-grain rice, both cultivar and location were significant for progeny production of R. dominica, but not RH, while cultivar and RH were significant for progeny production of S. oryzae, but not location. On both rice types, feeding damage of R. dominica followed the same trends and was always strongly positively correlated with progeny production (P < 0.05), but for S. oryzae there were several instances in which progeny production was not correlated with feeding damage (P ≥ 0.05). Physical characteristics of both rice types were statistically significant (P < 0.01) but actual numerical differences were extremely small, and were generally not correlated with progeny production of either species. Results indicate that the location in which a particular rice cultivar is grown, along with its characteristics, could affect susceptibility of the rice to R. dominica and S. oryzae.

  7. A review of the genus Carchesiopygus Schedl (Coleoptera: Curculionidae: Platypodinae), with keys to species.

    Science.gov (United States)

    Beaver, Roger A; Sanguansub, Sunisa

    2015-01-01

    The ten species included by Schedl (1972) in the Oriental platypodine genus Carchesiopygus Schedl are reviewed. The genus is rediagnosed and restricted to the following species: Carchesiopygus wollastoni (Chapuis) (type species), C. alternantes Schedl, C. assamensis (Beeson), C. impariporus (Beeson), C. multidentatus (Strohmeyer) and C. oculatus (Beeson), and distinguished from Crossotarsus Chapuis and related genera. The following species are transferred to Platypus Herbst: Carchesiopygus dentipennis Schedl, Carchesiopygus lobacanthus Schedl, Crossotarsus acanthurus Beeson, Crossotarsus psilacanthurus Beeson. Keys are provided to males and females of Carchesiopygus. The taxonomy, distribution and biology of the species are briefly reviewed, and some new records included. PMID:25781834

  8. Utility of morphological and molecular techniques for determination of paternity in two subspecies of Diabrotica undecimpunctata (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    An experiment was conducted to determine the paternity of F1 progeny using morphological and molecular methods in Diabrotica (Coleoptera: Chrysomelidae) subspecies: Diabrotica undecimpunctata howardi Barber, also known as spotted cucumber beetle and D. undecimpunctata undecimpunctata Mannerheim, als...

  9. Parasitylenchus sp. (Tylenchomorpha: Allantonematidae) parasitizing field populations of Harmonia axyridis (Coleoptera: Coccinellidae)

    DEFF Research Database (Denmark)

    Harding, Susanne; Poinar, George O. Jr.; Dimitrova, Desislava V.; Steenberg, Tove

    2011-01-01

    Adults of the invasive harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae), were found to be parasitized by nematodes (Tylenchomorpha: Allantonematidae) in Denmark. The nematodes were identified as Parasitylenchus sp. Major morphological characters of the nematodes did not differ si...

  10. Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae), information on a little known taxon from Colombia

    OpenAIRE

    Kippenhan, Mike

    2011-01-01

    Two specimens of Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae) in the Field Museum of Natural History offer additional information on the morphology and distribution of this rare species. One of the specimens, a female, is considered to be the first known specimen of this species.

  11. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae from Korea

    Directory of Open Access Journals (Sweden)

    Ik Je Choi

    2016-03-01

    Full Text Available A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  12. Patterns of tree species usage by long-horned beetles (Coleoptera: Cerambycidae) in Fiji

    Czech Academy of Sciences Publication Activity Database

    Waqa-Sakiti, H.; Stewart, A.; Čížek, Lukáš; Hodge, S.

    2014-01-01

    Roč. 68, č. 1 (2014), s. 57-64. ISSN 0030-8870 R&D Projects: GA ČR GAP504/12/1952 Institutional support: RVO:60077344 Keywords : Coleoptera Subject RIV: EH - Ecology, Behaviour Impact factor: 0.924, year: 2014 http://www.bioone.org/doi/pdf/10.2984/68.1.5

  13. An illustrated checklist of dung beetles (Coleoptera: Scarabaeinae from the Periyar Tiger Reserve, Kerala, India

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    Nithya Sathiandran

    2015-12-01

    Full Text Available An illustrated checklist of 36 species of dung beetles (Coleoptera: Scarabaeinae from the Periyar Tiger Reserve in the southern Western Ghats is presented.  Records of eight species endemic to the Western Ghats and a rare primitive old world dung beetle group, Ochicanthon nitidus (Paulian, from the forests of Periyar Tiger Reserve are provided. 

  14. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae) from Korea

    OpenAIRE

    Ik Je Choi; Jongok Lim; Jinyoung Park; Ji Hwan Park; Jong Kyun Park

    2016-01-01

    A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae) is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  15. Ground beetle (Coleoptera: Carabidae) assemblages in the Conservation Reserve Program crop rotation systems in Interior Alaska

    Science.gov (United States)

    Adult ground beetles (Coleoptera: Carabidae) abundance and diversity were documented on Conservation Research Program (CRP) agricultural lands in Delta Junction, Alaska (64ºN, 145º W). Twenty species were documented based on a total sample of 6,116 specimens collected during 2006 and 2007. Two speci...

  16. ESPECIES DE DRYOPHTHORINAE (COLEOPTERA: CURCULIONIDAE ASOCIADAS A PLÁTANO Y BANANO (Musaspp. EN COLOMBIA Dryophthorinae Species (Coleoptera: Curculionidae Associted to Platain and Banana crops (Musaspp. in Colombia

    Directory of Open Access Journals (Sweden)

    PAULA A SEPÚLVEDA-CANO

    Full Text Available Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.This synopsis is about beetle&#’;s subfamily Dryophthorinae (Coleoptera: Curculionidae associated to plantain and banana crops. Additionally keys illustrated for the species of the country are offered. Six species associated to these cultures are registered: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.

  17. Multistate characters and diet shifts: evolution of Erotylidae (Coleoptera).

    Science.gov (United States)

    Leschen, Richard A B; Buckley, Thomas R

    2007-02-01

    The dominance of angiosperms has played a direct role in the diversification of insects, especially Coleoptera. The shift to angiosperm feeding from other diets is likely to have increased the rate of speciation in Phytophaga. However, Phytophaga is only one of many hyperdiverse lineages of beetles and studies of host-shift proliferation have been somewhat limited to groups that primitively feed on plants. We have studied the diet-diverse beetle family Erotylidae (Cucujoidea) to determine if diet is correlated with high diversification rates and morphological evolution by first reconstructing ancestral diets and then testing for associations between diet and species number and diet and ovipositor type. A Bayesian phylogenetic analysis of morphological data that was previously published in Leschen (2003, Pages 1-108 in Fauna of New Zealand, 47; 53 terminal taxa and 1 outgroup, 120 adult characters and 1 diet character) yielded results that are similar to the parsimony analyses of Leschen (2003). Ancestral state reconstructions based on Bayesian and parsimony inference were largely congruent and both reconstructed microfungal feeding (the diet of the outgroup Biphyllidae) at the root of the Erotylidae tree. Shifts among microfungal, saprophagous, and phytophagous diets were most frequent. The largest numbers of species are contained in lineages that are macrofungal feeders (subfamily Erotylinae) and phytophagous (derived Languriinae), although the Bayesian posterior predictive tests of character state correlation were unable to detect any significant associations. Ovipositor morphology correlated with diet (i.e., acute forms were associated with phytophagy and unspecialized forms were associated with a mixture of diets). Although there is a general trend to increased species number associated with the shift from microfungal feeding to phytophagy (based on character mapping and mainly restricted to shifts in Languriinae), there is a large radiation of taxa feeding on

  18. Biomassa de Dynastinae (Coleoptera, Scarabaeidae fototácticos: um ensaio biogeográfico / Biomass of phototactic Dynastinae (Coleoptera, Scarabaeidae: a biogeographical assay

    Directory of Open Access Journals (Sweden)

    Paulo Jorge Riehs

    2006-04-01

    Full Text Available São apresentados e analisados valores da biomassa de Dynastinae (Coleoptera, Scarabaeidae fototácticos coletados em seis localidades do Leste e Centro-Oeste do Paraná, sul do Brasil, demonstrando-se as diferenças entre as diversas comunidades. Os dados obtidos no Paraná são correlacionados com os de Santa Maria, Rio Grande do Sul.

  19. Molecular Markers Detect Cryptic Predation on Coffee Berry Borer (Coleoptera: Curculionidae) by Silvanid and Laemophloeid Flat Bark Beetles (Coleoptera: Silvanidae, Laemophloeidae) in Coffee Beans.

    Science.gov (United States)

    Sim, Sheina B; Yoneishi, Nicole M; Brill, Eva; Geib, Scott M; Follett, Peter A

    2016-02-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is a serious pest of coffee worldwide. It was first detected in Hawai'i in 2010. Two predatory beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and Leptophloeus sp. (Coleoptera: Laemophloeidae), have been observed in H. hampei-infested coffee. Under laboratory conditions, colony-reared C. quadricollis and Leptophloeus sp. prey upon all life stages of H. hampei. However, the H. hampei life cycle occurs almost exclusively within a coffee bean obscured from direct observation. Thus, it is unknown if C. quadricollis and Leptophloeus sp. consume H. hampei as prey in the wild. To demonstrate predation of H. hampei by C. quadricollis and Leptophloeus sp., a molecular assay was developed utilizing species-specific primers targeting short regions of the mitochondrial COI gene to determine species presence. Using these primers, wild C. quadricollis and Leptophloeus sp. were collected and screened for the presence of H. hampei DNA using PCR. Analysis of collections from five coffee farms revealed predation of C. quadricollis and Leptophloeus sp. on H. hampei. Further laboratory testing showed that H. hampei DNA could be detected in predators for as long as 48 h after feeding, indicating the farm-caught predators had preyed on H. hampei within 2 d of sampling. This study demonstrates the utility of molecular markers for the study of the ecology of predators and prey with cryptic behavior, and suggests C. quadricollis and Leptophloeus sp. might be useful biocontrol agents against H. hampei. PMID:26487745

  20. Suscetibilidade de Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae ao enxofre Susceptibility of Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae to sulfur

    Directory of Open Access Journals (Sweden)

    José Roberto Gonçalves

    2007-08-01

    Full Text Available As criações de Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae são freqüentemente infestadas pelo ácaro Acarophenax lacunatus (Cross e Krantz (Prostigmata: Acarophenacidae. O objetivo deste trabalho foi avaliar doses de enxofre, acaricida eficaz contra A. lacunatus, não-prejudiciais ao desenvolvimento de R. dominica. As unidades experimentais foram constituídas de placas de Petri contendo 30 g de grãos de trigo infestados com 30 adultos de R. dominica. Os tratamentos consistiram na utilização de doses de enxofre sobre os grãos, correspondentes a 0,0; 0,6; 0,9; 1,2; 1,5; 3,0; 6,0; 12,0; 24,0 e 48,0mg i a g-1, em dez repetições. As unidades experimentais foram armazenadas por 60 dias a 30±1°C, 60±5% UR e escotofase de 24h. O desenvolvimento de R. dominica foi afetado pela utilização de doses de enxofre maiores que 3,0mg i a g-1.The laboratory rearing of Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae is frequently infested by the parasite mite Acarophenax lacunatus (Cross and Krantz (Prostigmata: Acarophenacidae. This study was aimed at evaluating the sulfur doses, an effective acaricide against A. lacunatus, not harmful to the development of with R. dominica. The experimental units were Petri dishes containing 30g of whole wheat grains powdered with the different doses of the sulfur (0.0, 0.6, 0.9, 1.2, 1.5, 3.0, 6.0, 12.0, 24.0 and 48.0mg a i g-1 infested with 30 adults of R. dominica, in ten replicates. All treatments were maintained under controlled conditions (30±1°C, 60±5% r h and 24h scotophase for 60 days after the insect infestation. Sulfur doses higher than 3.0mg a i g-1 negatively affected R. dominica development.

  1. The complete mitogenome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae).

    Science.gov (United States)

    Nan, Xiaoning; Wei, Cong; He, Hong

    2016-05-01

    The complete mitochondrial genome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae) were reconstructed from whole-genome Illumina Hiseq 2000 sequencing data with an average coverage of 1406.7X. The circular genome is 15,122 bp in length, consisting of 13 protein-coding genes (PCGs), 21 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and one D-loop or control region. The tRNA-Ile gene was not found in the mitochondrial genome, as is identical to two other curculionidae species, i.e. Sphenophorus sp. (GU176342) and Naupactus xanthographus (GU176345). All PCGs initiated with ATN codons, except for the ND1 started with TTG. Two PCGs (COI and ND4) have an incomplete stop codon T. Two PCGs (ND4L and ND1) harbor the stop codon TAG, while all other PCGs terminated with the TAA codon. The nucleotide composition is highly asymmetric (38.7% A, 14.4% C, 9.2% G and 37.8% T) with an overall AT content of 76.5%. PMID:25427809

  2. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae) *

    Science.gov (United States)

    Petitpierre, Eduard

    2011-01-01

    Abstract Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xyp and XYp clearly prevailing (79.0%), but with the XO well represented too (19.75%). The modal haploid number for chrysomelines is n = 12 (34.2%) although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6%) and in subtribe Chrysomelina n = 17 (65.7%). Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74) has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry. PMID:22303104

  3. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae).

    Science.gov (United States)

    Petitpierre, Eduard

    2011-01-01

    Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xy(p) and XY(p) clearly prevailing (79.0%), but with the XO well represented too (19.75%). The modal haploid number for chrysomelines is n = 12 (34.2%) although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6%) and in subtribe Chrysomelina n = 17 (65.7%). Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74) has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry. PMID:22303104

  4. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Eduard Petitpierre

    2011-12-01

    Full Text Available Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xyp and XYp clearly prevailing (79.0%, but with the XO well represented too (19.75%. The modal haploid number for chrysomelines is n = 12 (34.2% although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6% and in subtribe Chrysomelina n = 17 (65.7%. Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74 has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry.

  5. Plant compounds insecticide activity against Coleoptera pests of stored products

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Marcio Dionizio; Picanco, Marcelo Coutinho; Guedes, Raul Narciso Carvalho; Campos, Mateus Ribeiro de; Silva, Gerson Adriano; Martins, Julio Claudio [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Biologia Animal]. E-mail: marcio.dionizio@gmail.com; picanco@ufv.br; guedes@ufv.br; mateusc3@yahoo.com.br; agronomiasilva@yahoo.com.br

    2007-07-15

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD{sub 50} from 2.72 to 39.71 mg g{sup -1} a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis. (author)

  6. Economic analysis of emerald ash borer (Coleoptera: Buprestidae) management options.

    Science.gov (United States)

    Vannatta, A R; Hauer, R H; Schuettpelz, N M

    2012-02-01

    Emerald ash borer, Agrilus planipennis (Fairmaire) (Coleoptera: Buprestidae), plays a significant role in the health and extent of management of native North American ash species in urban forests. An economic analysis of management options was performed to aid decision makers in preparing for likely future infestations. Separate ash tree population valuations were derived from the i-Tree Streets program and the Council of Tree and Landscape Appraisers (CTLA) methodology. A relative economic analysis was used to compare a control option (do-nothing approach, only removing ash trees as they die) to three distinct management options: 1) preemptive removal of all ash trees over a 5 yr period, 2) preemptive removal of all ash trees and replacement with comparable nonash trees, or 3) treating the entire population of ash trees with insecticides to minimize mortality. For each valuation and management option, an annual analysis was performed for both the remaining ash tree population and those lost to emerald ash borer. Retention of ash trees using insecticide treatments typically retained greater urban forest value, followed by doing nothing (control), which was better than preemptive removal and replacement. Preemptive removal without tree replacement, which was the least expensive management option, also provided the lowest net urban forest value over the 20-yr simulation. A "no emerald ash borer" scenario was modeled to further serve as a benchmark for each management option and provide a level of economic justification for regulatory programs aimed at slowing the movement of emerald ash borer. PMID:22420272

  7. Identification of G protein-coupled receptors required for vitellogenin uptake into the oocytes of the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Bai, Hua; Palli, Subba Reddy

    2016-01-01

    Previous studies suggested that a membrane receptor might be involved in mediating vitellogenin (Vg) uptake and juvenile hormone (JH)-regulated remodeling of follicular epithelium (also called 'patency'). G protein-coupled receptor (GPCR) family is one of the largest membrane receptor protein families and controls many key physiological processes. To investigate the role of GPCRs in insect reproduction and juvenile hormone-regulated Vg uptake, we performed a comprehensive RNA interference (RNAi) screen targeting GPCRs in the red flour beetle, Tribolium castaneum. Out of 112 GPCRs tested, knockdown of 41 GPCRs resulted in a reduction in fecundity. Interestingly, RNAi against two GPCRs (a Rhodopsin-like receptor and a Dopamine D2-like receptor) led to a significant reduction in Vg accumulation in developing oocytes. Functional assays of these two GPCRs showed that JH triggers a dose-dependent inhibition of intracellular cAMP levels in HEK293 cells expressing Tribolium Dopamine D2-like receptor. These data suggest that Dopamine D2-like receptor plays crucial roles in regulating Vg uptake and is a promising candidate membrane receptor mediating JH regulation of patency in the red flour beetle. PMID:27277501

  8. The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference

    Directory of Open Access Journals (Sweden)

    Da Xiao

    2014-04-01

    Full Text Available We identified and characterized the TcLgl gene putatively encoding lethal giant larvae (Lgl protein from the red flour beetle (Tribolium castaneum. Analyses of developmental stage and tissue-specific expression patterns revealed that TcLgl was constitutively expressed. To examine the role of TcLgl in insect development, RNA interference was performed in early (1-day larvae, late (20-day larvae, and early (1-day pupae. The early larvae injected with double-stranded RNA of TcLgl (dsTcLgl at 100, 200, and 400 ng/larva failed to pupate, and 100% mortality was achieved within 20 days after the injection or before the pupation. The late larvae injected with dsTcLgl at these doses reduced the pupation rates to only 50.3%, 36.0%, and 18.2%, respectively. The un-pupated larvae gradually died after one week, and visually unaffected pupae failed to emerge into adults and died during the pupal stage. Similarly, when early pupae were injected with dsTcLgl at these doses, the normal eclosion rates were reduced to only 22.5%, 18.0%, and 11.2%, respectively, on day 7 after the injection, and all the adults with abnormal eclosion died in two days after the eclosion. These results indicate that TcLgl plays an essential role in insect development, especially during their metamorphosis.

  9. A dual role for nanos and pumilio in anterior and posterior blastodermal patterning of the short-germ beetle Tribolium castaneum.

    Science.gov (United States)

    Schmitt-Engel, Christian; Cerny, Alexander C; Schoppmeier, Michael

    2012-04-15

    Abdominal patterning in Drosophila requires the function of Nanos (nos) and Pumilio (pum) to repress posterior translation of hunchback mRNA. Here we provide the first functional analysis of nanos and pumilio genes during blastodermal patterning of a short-germ insect. We found that nos and pum in the red flour beetle Tribolium castaneum crucially contribute to posterior segmentation by preventing hunchback translation. While this function seems to be conserved among insects, we provide evidence that Nos and Pum may also act on giant expression, another gap gene. After depletion of nos and pum by parental RNAi, Hunchback and giant remain ectopically at the posterior blastoderm and the posterior Krüppel (Kr) domain is not being activated. giant may be a direct target of Nanos and Pumilio in Tribolium and presumably prevents early Kr expression. In the absence of Kr, the majority of secondary gap gene domains fail to be activated, and abdominal segmentation is terminated prematurely. Surprisingly, we found Nos and Pum also to be involved in early head patterning, as the loss of Nos and Pum results in deletions and transformations of gnathal and pre-gnathal anlagen. Since the targets of Nos and Pum in head development remain to be identified, we propose that anterior patterning in Tribolium may involve additional maternal factors. PMID:22326441

  10. Infection of Tribolium castaneum with Bacillus thuringiensis: quantification of bacterial replication within cadavers, transmission via cannibalism, and inhibition of spore germination.

    Science.gov (United States)

    Milutinović, Barbara; Höfling, Christina; Futo, Momir; Scharsack, Jörn P; Kurtz, Joachim

    2015-12-01

    Reproduction within a host and transmission to the next host are crucial for the virulence and fitness of pathogens. Nevertheless, basic knowledge about such parameters is often missing from the literature, even for well-studied bacteria, such as Bacillus thuringiensis, an endospore-forming insect pathogen, which infects its hosts via the oral route. To characterize bacterial replication success, we made use of an experimental oral infection system for the red flour beetle Tribolium castaneum and developed a flow cytometric assay for the quantification of both spore ingestion by the individual beetle larvae and the resulting spore load after bacterial replication and resporulation within cadavers. On average, spore numbers increased 460-fold, showing that Bacillus thuringiensis grows and replicates successfully in insect cadavers. By inoculating cadaver-derived spores and spores from bacterial stock cultures into nutrient medium, we next investigated outgrowth characteristics of vegetative cells and found that cadaver-derived bacteria showed reduced growth compared to bacteria from the stock cultures. Interestingly, this reduced growth was a consequence of inhibited spore germination, probably originating from the host and resulting in reduced host mortality in subsequent infections by cadaver-derived spores. Nevertheless, we further showed that Bacillus thuringiensis transmission was possible via larval cannibalism when no other food was offered. These results contribute to our understanding of the ecology of Bacillus thuringiensis as an insect pathogen. PMID:26386058

  11. The negative effect of starvation and the positive effect of mild thermal stress on thermal tolerance of the red flour beetle, Tribolium castaneum

    Science.gov (United States)

    Scharf, Inon; Wexler, Yonatan; MacMillan, Heath Andrew; Presman, Shira; Simson, Eddie; Rosenstein, Shai

    2016-04-01

    The thermal tolerance of a terrestrial insect species can vary as a result of differences in population origin, developmental stage, age, and sex, as well as via phenotypic plasticity induced in response to changes in the abiotic environment. Here, we studied the effects of both starvation and mild cold and heat shocks on the thermal tolerance of the red flour beetle, Tribolium castaneum. Starvation led to impaired cold tolerance, measured as chill coma recovery time, and this effect, which was stronger in males than females, persisted for longer than 2 days but less than 7 days. Heat tolerance, measured as heat knockdown time, was not affected by starvation. Our results highlight the difficulty faced by insects when encountering multiple stressors simultaneously and indicate physiological trade-offs. Both mild cold and heat shocks led to improved heat tolerance in both sexes. It could be that both mild shocks lead to the expression of heat shock proteins, enhancing heat tolerance in the short run. Cold tolerance was not affected by previous mild cold shock, suggesting that such a cold shock, as a single event, causes little stress and hence elicits only weak physiological reaction. However, previous mild heat stress led to improved cold tolerance but only in males. Our results point to both hardening and cross-tolerance between cold and heat shocks.

  12. Properties and natural occurrence of maternal-effect selfish genes ('Medea' factors) in the red flour beetle, Tribolium castaneum

    International Nuclear Information System (INIS)

    Maternally acting selfish genes, termed 'Medea' factors, were found to be widespread in wild populations of Tribolium castaneum collected in Europe, North and South America, Africa and south-east Asia, but were rare or absent in populations from Australia and the Indian subcontinent. We detected at least four distinct genetic loci in at least two different linkage groups that exhibit the Medea pattern of differential mortality of genotypes within maternal families. Although each M factor tested had similar properties of maternal lethality to larvae and zygotic self-rescue, M factors representing distinct loci did not show cross-rescue. Alleles at two of these loci, M1 and M4, were by far the most prevalent, M4 being the predominant type. M2 and M3 were each found only once, in Pakistan and Japan, respectively. Although M1 could be genetically segregated from M4 and maintained as a purified stock, the M1 factor invariably co-occurred with M4 in field populations, whereas M4 usually occurred in the absence of other Medea factors. The dominant maternal lethal action of M1 could be selectively inactivated (reverted) by gene-knockout gamma irradiation with retention of zygotic rescue activity. (author)

  13. Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae

    Directory of Open Access Journals (Sweden)

    Elias Soares Gomes

    2014-06-01

    Full Text Available Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae. Coleopterans of the family Melolonthidae comprise a large group of species that feed on different food sources, including plant roots, stems, and leaves, in addition to plant materials at different decomposition stages. Several species are found in the genus Leucothyreus, occurring in different regions of Brazil, including the various biomes in the country. Information on the biology of species of the genus Leucothyreus is scarce, therefore, we conducted studies on the biological aspects of Leucothyreus ambrosius Blanchard, 1850. The period of adult occurrence was determined with a light trap installed between a cropped and pasture area in the municipality of Aquidauana, Mato Grosso do Sul State, Brazil. Adults collected in the field were used to form insect pairs and the studies were initiated in the entomology laboratory as the adults began ovipositing. Adults were observed flying in the field from October to December. Eggs were obtained as pairs were formed and a colony was established, the embryonic period lasting 14.6 days on average. The larval period in the 1st instar lasted 21.6 days, in the 2nd instar 19.6 days, and in the 3rd instar, 85.6 days. The head capsule width was 1.48 mm in the 1st instar, 2.44 mm in the 2nd, and 3.83 mm in 3rd larval instar. The pupal stage had an average duration of 35.5 days. The egg to adult period lasted 173.3 days. Morphometric information for the larval and adult stages is presented in this study.

  14. Contributions to the knowledge of Atlantic Canadian Histeridae (Coleoptera

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    Christopher Majka

    2008-09-01

    Full Text Available New records of Histeridae from Atlantic Canada are reported. Three species are newly recorded from Prince Edward Island and two from New Brunswick, one of which, the introduced Palearctic Atholus bimaculatus (Linnaeus, is newly recorded from Atlantic Canada as a whole. These new records increase the known histerid fauna of the region to 37 species, 30 native and 7 introduced ones. The regional zoogeography of the Histeridae is examined focusing on differences between the faunal composition of the various provinces and the possible reasons responsible for these. The island faunas of Cape Breton, Prince Edward Island, and Newfoundland are examined. All have reduced faunas in comparison with the mainland perhaps as a result of island-associated diminutions, an area effect, a paucity of collecting, or a combination of these factors. Those of Cape Breton and Prince Edward Island are proportionately similar to those of other families of Coleoptera, whereas that of Newfoundland (only 10% of the mainland fauna is significantly less, a circumstance which deserves further investigation. Seven species of introduced histerids have been recorded in the region. The average dates of first detection of these species are much later than the earliest records of these species in North America and comparatively later than is the case with other suites of introduced species in the Staphylinidae and Carabidae, perhaps as a result of the sparse attention the Histeridae have historically received by coleopterists in the region. Most of the introduced histerids are known to be synanthropic and may have been introduced to the region association with the importation of livestock and materials related to animal husbandry. The Histeridae of the region largely fall into one of several trophic guilds: coastal species and those associated with beach-drift material; species associated with bird nests; species associated with mammal nests; myrmecophilus species; saproxylic

  15. Biocontrol of larval mosquitoes by Acilius sulcatus (Coleoptera: Dytiscidae

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    Banerjee Siddhartha S

    2008-10-01

    Full Text Available Abstract Background Problems associated with resistant mosquitoes and the effects on non-target species by chemicals, evoke a reason to find alternative methods to control mosquitoes, like the use of natural predators. In this regard, aquatic coleopterans have been explored less compared to other insect predators. In the present study, an evaluation of the role of the larvae of Acilius sulcatus Linnaeus 1758 (Coleoptera: Dytiscidae as predator of mosquito immatures was made in the laboratory. Its efficacy under field condition was also determined to emphasize its potential as bio-control agent of mosquitoes. Methods In the laboratory, the predation potential of the larvae of A. sulcatus was assessed using the larvae of Culex quinquefasciatus Say 1823 (Diptera: Culicidae as prey at varying predator and prey densities and available space. Under field conditions, the effectiveness of the larvae of A. sulcatus was evaluated through augmentative release in ten cemented tanks hosting immatures of different mosquito species at varying density. The dip density changes in the mosquito immatures were used as indicator for the effectiveness of A. sulcatus larvae. Results A single larva of A. sulcatus consumed on an average 34 IV instar larvae of Cx. quinquefasciatus in a 24 h period. It was observed that feeding rate of A. sulcatus did not differ between the light-on (6 a.m. – 6 p.m., and dark (6 p.m. – 6 a.m. phases, but decreased with the volume of water i.e., space availability. The prey consumption of the larvae of A. sulcatus differed significantly (P A. sulcatus larvae, while with the withdrawal, a significant increase (p A. sulcatus in regulating mosquito immatures. In the control tanks, mean larval density did not differ (p > 0.05 throughout the study period. Conclusion the larvae of the dytiscid beetle A. sulcatus proved to be an efficient predator of mosquito immatures and may be useful in biocontrol of medically important mosquitoes.

  16. Using malaise traps to sample ground beetles (Coleoptera: Carabidae).

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D., James L. Hanula, and Scott Horn

    2005-01-01

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  17. Using malaise traps to sample ground beetles (Coleoptera. Carabidae)

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Hanula, James L. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Horn, Scott [USDA Forest Service, Savannah River, New Ellenton, SC (United States)

    2012-04-02

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  18. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Dilma Solange Napp

    2006-01-01

    Full Text Available Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae. Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae. The following new genus and species, all from Bolivia, are described in Compsocerini: Ecoporanga gen. nov., type species E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., from Santa Cruz, and Ethemon iuba sp. nov. from Cochabamba.

  19. Una especie nueva de Trechisibus de la Argentina (Coleoptera: Carabidae A new species of Trechisibus from Argentina (Coleoptera: Carabidae

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    Sergio Roig-Juñent

    2005-12-01

    Full Text Available RESUMEN. Las Trechini constituyen una tribu de Carabidae distribuida ampliamente en la región Andino Patagónica. A pesar de que existen varias revisiones parciales de esta tribu para la Argentina y Chile, es muy común hallar especies nuevas, debido sobre todo a la prospección de áreas no exploradas. En este aporte se describe una especie nueva del género Trechisibus Jeannel del Cerro Nevado (Mendoza, Argentina. Por sus características morfológicas pertenece al grupo de especies depressus. Se describe e ilustra el adulto de esta especie nueva, se provee una clave para la identificación de las especies del grupo depressus y se discuten algunos aspectos de la distribución de estas especies.ABSTRACT. Trechini is a tribe of Carabidae (Coleoptera widely distributed in the Andean Patagonian region in South America. In spite of several partial revisions of the tribe for Argentina and Chile, it is very common to find new species due to the research of unexplored areas. In the present paper, a new species of the genus Trechisibus Jeannel from the Cerro Nevado (Mendoza, Argentina is described. Based on its morphological features the new species is considered as a member of the depressus group. The adult of the new species is described and illustrated, a key for the identification of the species of the depressus group is provided, and some aspects of the distribution of the group are discussed.

  20. Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil

    OpenAIRE

    Renata C. Campos; Malva I. Medina Hernández

    2013-01-01

    Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil. The beetles of the subfamily Scarabaeinae are important organisms that participate in the cycle of decomposition, especially in tropical ecosystems. Most species feed on feces (dung) or carcasses (carrion) and are associated with animals that produce their food resources. Dung beetles are divided into three functional groups: rollers, tunnelers and dwellers. This present work aims to study the ...

  1. Species of beetles (Coleoptera; Scarabaeidae associated to banana (Musa spp. in Ceballos, Ciego de Avila, Cuba

    Directory of Open Access Journals (Sweden)

    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in bananas plantations, according to Sisne, 2009 and MINAG, 1985, in the Citric enterprise of Ciego de Ávila during the period between May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species Cyclocephala cubana Chapin, Phyllophaga puberula Duval, and Phyllophaga patruelis Chev. are associated to bananas crops in these areas.

  2. Ecological impact of entomopathogenic nematodes used to control the large pine weevil, Hylobius abietis (Coleoptera: Curculionidae)

    OpenAIRE

    Harvey, Christopher D.

    2010-01-01

    The large pine weevil (Hylobius abietis; Coleoptera: Curculionidae) is one of the most economically damaging pests in forestry across Northern Europe. Its larvae develop in coniferous tree stumps on clearfell sites and adult weevils feed on seedlings that are replanted on these sites, causing substantial mortality. As the drive towards achieving the sustainable management of natural resources increases, biological control agents are being considered as an alternative to chemical ...

  3. Otiorhynchus spp. (Coleoptera: Curculionidae) as pests in horticulture: genetics and management options with entomopathogenic fungi

    OpenAIRE

    Hirsch, Jacqueline

    2012-01-01

    Worldwide, weevils of the genus Otiorhynchus (Coleoptera: Curculionidae) cause damage with detrimental economic effects to many horticultural crops due to the root feeding of their larvae as well as foliage feeding of their adults. Aside from the black vine weevil Otiorhynchus sulcatus, which is the best-known pest within this genus, numerous other Otiorhynchus species have been increasingly recognized as pests in recent years. Nocturnal adult weevils and soil-inhibiting larvae are in princip...

  4. Shared Ancestry of Symbionts? Sagrinae and Donaciinae (Coleoptera, Chrysomelidae) Harbor Similar Bacteria

    OpenAIRE

    Dimitra Synefiaridou; Gregor Kölsch

    2012-01-01

    When symbioses between insects and bacteria are discussed, the origin of a given association is regularly of interest. We examined the evolution of the symbiosis between reed beetles (Coleoptera, Chrysomelidae, Donaciinae) and intracellular symbionts belonging to the Enterobacteriaceae. We analyzed the partial sequence of the 16S rRNA to assess the phylogenetic relationships with bacteria we found in other beetle groups (Cerambycidae, Anobiidae, other Chrysomelidae). We discuss the ecology of...

  5. Laboratory Rearing of Laricobius nigrinus (Coleoptera: Derodontidae): A Predator of the Hemlock Woolly Adelgid (Hemiptera: Adelgidae)

    OpenAIRE

    Salom, S. M.; L. T. Kok; Lamb, A B; Jubb, C

    2012-01-01

    Coleopteran species are biological control agents of numerous invasive pests. Laricobius nigrinus (Coleoptera: Derodontidae), a predaceous, univoltine species, spends the summer aestivating but is active for the rest of the year. Laricobius nigrinus possesses many essential attributes for effective biological control of the hemlock woolly adelgid (Hemiptera: Adelgidae). The predator must be reared in large numbers for field releases. We describe some of the studies that led to the successful ...

  6. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae)

    OpenAIRE

    Andréa Roveré Franz; Neiva Knaak; Lidia Mariana Fiuza

    2011-01-01

    Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae). Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf), Zingiber officinale (root) and Mentha sp. (leaf). The efficacy of ...

  7. New species and records of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae: Macrodactylini) from Bolivia.

    Science.gov (United States)

    Arce-Pérez, Roberto; Morón, Miguel Ángel

    2014-01-01

    Two new species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae) from Bolivia are described and illustrated: M. megaphyllus new species (from Comarapa, Santa Cruz and Sehuenca, Cochabamba) and M. yunganus new species (from Mairana and Comarapa, Santa Cruz). In addition, the species Macrodactylus bolivianus Moser, M. gracilis Moser, and M. nobilis Frey are redescribed and illustrated to help facilitate identification of these species. A key to the 10 species of Macrodactylus presently known from Bolivia is provided.  PMID:25283110

  8. Wireworms’ Management: An Overview of the Existing Methods, with Particular Regards to Agriotes spp. (Coleoptera: Elateridae

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    Fanny Barsics

    2013-01-01

    Full Text Available Wireworms (Coleoptera: Elateridae are important soil dwelling pests worldwide causing yield losses in many crops. The progressive restrictions in the matter of efficient synthetic chemicals for health and environmental care brought out the need for alternative management techniques. This paper summarizes the main potential tools that have been studied up to now and that could be applied together in integrated pest management systems and suggests guidelines for future research.

  9. Lectotype designations and nomenclatural changes in Xylographus Mellié (Coleoptera, Ciidae)

    OpenAIRE

    Sandoval-Gómez,Vivian E.; Cristiano Lopes-Andrade; John Lawrence

    2014-01-01

    We designate lectotypes and propose nomenclatural changes in Xylographus Mellié (Coleoptera, Ciidae) based on type specimens deposited in the Museum of Comparative Zoology (USA), Museum für Naturkunde Berlin (Germany), the Natural History Museum (UK), Muséum d’Histoire Naturelle de la Ville de Genève (Switzerland), Muséum National d’Histoire Naturelle (France), Naturhistoriska Riksmuseet (Sweden) and Naturhistorisches Museum Wien (Austria). We desi...

  10. The Leaf-Beetles (Coleoptera, Chrysomelidae) Feed On Some Weeds In Tokat Province

    OpenAIRE

    Çam, Halit; ATAY, Turgut

    2004-01-01

    This study was carried out to determine the leaf-beetles (Coleoptera, Chrysomelidae) which causes extensive damage on weeds in the vicinity of Tokat, Turkey. Total 9 taxa including 4 species of Chrysomelinae, 1 species of Clytrinae, 1 species of Criocerinae, 2 species of Alticinae and 1 species of Cassidinae were found to be destructive on different weed species. These species were; Entomoscelis adonidis (Pall.) on Sinapis arvensis L., Gastrophysa polygoni (L.) on Polygonum convolvulus L. an...

  11. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern

    Directory of Open Access Journals (Sweden)

    SUGIYARTO

    2009-07-01

    Full Text Available Maryati KT, Sugiyarto. 2010. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern. Nusantara Bioscience 1: 72-77. This research aims to find out the white grub (Melolonthidae; Coleoptera variability based on the morphological characteristic and protein banding pattern found in ”salak pondoh” farm in Regencies of Sleman, Yogyakarta and Magelang, Central Java. Each area has five sampling points. Morphological analysis on white grub was conducted using descriptive method and analysis on protein banding pattern was conducted using qualitative analysis based on the presence or absent of band pattern on the gel, and qualitatively based on the relative mobility value (Rf of protein. The result indicated that the white grub in Sleman and Magelang, based on morphology characteristic is only one species, namely Holothricia sp. Based on the protein banding pattern, the white grub sample have differences of protein band number and protein molecular weight. Key words: Salacca zalacca, white grub, morphology, protein banding pattern.Abstrak. Maryati KT, Sugiyarto. 2010. Karakterisasi lundi putih (Melolonthidae: Coleoptera pada pertanaman salak berdasarkan ciri morfologi dan pola pita protein. Nusantara Bioscience 1: 72-77. Penelitian ini bertujuan untuk mengetahui keanekaragaman lundi putih (Melolonthidae; Coleoptera berdasarkan ciri morfologi dan pola pita protein yang ditemukan di lahan pertanaman salak pondoh di Kabupaten Sleman, Yogyakarta dan Kabupaten Magelang, Jawa Tengah. Pada masing-masing wilayah diambil lima titik sampling. Analisis morfologi lundi putih digunakan metode deskriptif, dan analisis pola pita protein digunakan analisis kualitatif berdasarkan muncul tidaknya pola pita pada gel, dan secara kuantitatif berdasarkan nilai mobilitas relatif protein (RF. Hasil penelitian menunjukkan bahwa sampel lundi putih di Kabupaten Sleman dan Magelang, berdasar karakter

  12. A new genus and four new species of false click beetles (Coleoptera: Eucnemidae) from Southeast Asia

    OpenAIRE

    Otto, Robert L.

    2016-01-01

    A new genus and four new species of false click beetle (Coleoptera: Eucnemidae) are described. Bermilloides new genus is described from Bornean Malaysia. New species are: Calyptocerus iridis new species (Philippines), Bermilloides lumawigi new species (Malaysia), Spinifornax elongatus new species (Malaysia) and Ceratus antennatus new species (Thailand). Dorsal and ventral habitus, as possible, for each species are illustrated. Male aedeagi are illustrated for Calyptocerus iridis and Ceratu...

  13. Studies on the Dung-inhabiting Beetles (Insecta: Coleoptera Community of Western Anatolia, Turkey

    Directory of Open Access Journals (Sweden)

    Sinan Anlaş

    2011-12-01

    Full Text Available In Bozdağlar Mountain of western Turkey, the diversity and composition of the dung-inhabiting beetles in two locations situated in different altitudes (600 m and 900 m in 2004 and 2006 assemblages were sampled. A total of 5.709 individuals from 88 species belonging to the families Scarabaeidae, Aphodiidae, Geotrupidae, Carabidae, Hydrophilidae, Histeridae and Ptilidae of the order Coleoptera are recorded.

  14. Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil

    OpenAIRE

    Andreia Mauruto Chernaki-Leffer; Daniel Ricardo Sosa-Gómez; Almeida, Lúcia M.; Ivani de Oliveira Negrão Lopes

    2011-01-01

    Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer), is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvo...

  15. Novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae da Bolívia

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2008-01-01

    Full Text Available Novas espécies descritas da Bolívia, Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.New species of Onciderini (Coleoptera, Cerambycidae, Lamiinae from Bolivia. New species described from Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.

  16. Penggunaan Beberapa Serbuk Rimpang Terhadap Mortalitas Hama Callosobruchus chinensis L. (Coleoptera; Bruchidae) Pada Kacang Hijau

    OpenAIRE

    Hayati, Anni

    2012-01-01

    Anni Hayati, "Use of Some Powdered Rhizome Mortality Against Pests Callosobruchus chinensis L. (Coleoptera: Bruchidae) On the Green Bean ". It was under supervised by Yuswani Pangestiningsih and Fatimah Zahara. C.chinensis L. are pests on green beans in storage which causes considerable yield loss, one of effective control is the use of botanical insecticides. Use of some powdered rhizome on mortality C.chinensis L. in a green bean plant pests carried in the laboratory of t...

  17. Ernährungsphysiologische Aspekte xylophager Bockkäferlarven (Coleoptera: Cerambycidae)

    OpenAIRE

    Grünwald, Sebastian

    2009-01-01

    Mittels Fluoreszenz-in-situ-Hybridisierung und PCR-basierter rRNA-Sequenzierung wurde die Darm- und Myzetomflora xylophager Insektenlarven (Coleoptera: Cerambycidae) untersucht. Die Myzetome enthalten hefeartige Symbionten in Form von Candida rhagii und Candida shehatae. Das Darmlumen der Tiere beherbergt eine hoch variable Gemeinschaft von Bakterien und Pilzen, die zum Teil der Nahrung entstammt. Die Larven des Hausbockkäfers nehmen eine Sonderstellung ein: Ihnen fehlen sowohl Myzetome als a...

  18. Defensive Glands of the Darkling Beetle Mesomorphus villiger Blanchard (Coleoptera: Tenebrionidae)

    OpenAIRE

    Seena, C. M.; Sabu K. Thomas

    2013-01-01

    Massive home invasion by the darkling beetle Mesomorphus villiger Blanchard 1853 (Coleoptera: Tenebrionidae) during monsoon season make it a nuisance pest in many regions of south India. Morphology of defensive glands and mode of release and dispersal of the defensive secretion were analysed. Defensive glands were separated from the abdominal sternites by cutting along the posterior margin of the seventh sternite. Glands are evaginations of intersegmental membrane between the seventh and eigh...

  19. DIVERSITY OF CARABIDS (COLEOPTERA) ASSOCIATED WITH THE LOWER LURIN RIVER, LIMA, PERU

    OpenAIRE

    ARMANDO VÉLEZ-AZAÑERO; Alfonso Lizárraga-Travaglini

    2013-01-01

    Quarterly samples of beetles (Coleoptera: Carabidae) were taken associated with the lower basin of the Lurín River (Lima-Peru) during the period August 2009 - February 2011, in six sampling points between 5 and 51 masl. Pitfall traps were used and obtained a total of 59 specimens distributed among three tribes, three genera, and four morphospecies. We report the presence of Megacephala, Scarites genus, and Pterostichus with the latter being the predominant genus.

  20. New national and state records of Neotropical Staphylinidae (Insecta: Coleoptera).

    Science.gov (United States)

    Asiain, Julieta; Márquez, Juan; Irmler, Urlich

    2015-01-01

    Eighteen new national records of nine species of Osoriinae (Staphylinidae) are added for 10 Neotropical countries. Additionally, 17 species of three subfamilies are first recorded from ten States of México. The distributional patterns of the studied species are commented and the congruence with species of different families of Coleoptera and Odonata previously analyzed is discussed. Finally, we conclude that some of these patterns can be proposed as hypothesis of primary biogeographic homology. PMID:26249885

  1. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae) in Cotton

    OpenAIRE

    P. G. Tillman; Cottrell, T. E.

    2012-01-01

    Lady beetles (Coleoptera: Coccinellidae) prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L.), Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas), Coleomegilla maculata (De Geer), Cycloneda munda (Say), and Olla v-nigrum (Mulsant) were found in sorghum over both years. Lady beetle compositions in sorghum and ...

  2. A checklist of the genus Blosyrus Schoenherr (Coleoptera: Curculionidae: Entiminae of the world

    Directory of Open Access Journals (Sweden)

    G. Mahendiran

    2013-08-01

    Full Text Available An annotated checklist of Blosyrus Schoenherr (Coleoptera: Curculionidae: Entiminae comprising 101 species with their updated nomenclature, synonyms and distribution is given. The distribution pattern indicates that the genus is diversified mostly in the Sub-Saharan Africa and Asia regions. Out of 101 species, 92 occur in Sub-Saharan Africa. From India, eight species were recorded. In India, the distribution is mainly in West Bengal, Assam, Maharashtra, Andhra Pradesh, Karnataka and Tamil Nadu.

  3. Biological activities of Allium sativum essential oil against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae)

    OpenAIRE

    Chaubey Mukesh Kumar

    2014-01-01

    Essential oil from Allium sativum was isolated and investigated for its repellent, insecticidal, ovipositional and egg hatching inhibition activities against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). A. sativum essential oil repelled bruchid adults at a very low concentration in choice oviposition assay. A. sativum essential oil caused both fumigant and contact toxicity in C. chinensis adults in a concentration dependent manner. Oviposition potency of C. chinensis adults...

  4. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae)

    OpenAIRE

    M.M. Kiradoo; Meera Srivastava

    2011-01-01

    The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae) is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insect...

  5. Laboratory Bioassay of Iranian Isolates of Entomopathogenic Fungus Metarhizium anisopliae (Metsch. Sorokin (Ascomycota: Hypocreales against two Species of Storage Pest

    Directory of Open Access Journals (Sweden)

    Adel Khashaveh

    2013-03-01

    Full Text Available M-B 12.00 Normal 0 false false false EN-US X-NONE FA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";} The susceptibility of adults of Tribolium castaneum Herbst (Coleoptera: Tenebrionidae and Oryzaephilus surinamensis L. (Coleoptera: Silvanidae to three Iranian Isolates of the entomopathogenic fungi Metarhizium anisopliae (Metsch. Sorokin was evaluated through bioassays with direct immersion under laboratory conditions. For each isolates, five aqueous suspensions were prepared in a logarithmic series in Tween 80 (0.05% v/v. Results showed that adult of both species were susceptible to all isolates of M. anisopliae. For all three isolates, mortality percentage of the two species increased with increasing conidial concentration and significant difference was observed between concentrations. The corrected cumulative mortality of adult of O. surinamensis 10 days after immersion ranged from 12.38 to 85.84%, 18.6 to 62.83% and 10.63 to 77.87% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. These amounts for T. castaneum varied from 31.07 to 74.78%, 26.02 to 75.61% and 23.33 to 89.99% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. The parameters of probit analysis demonstrated non-overlap of 95% confidence limits of LC50 and LC95 and significant difference was observed among three isolates tested against each insect. The lowest and the highest LC50 and LC95 values were observed in the isolates DEMI001 for O. surinamensis (3/1×105 and 1/5×108 and IRAN 715C for T. castaneum (6.2×108 and 6.9×1014, respectively. This

  6. Especies mexicanas de Curculionidae (Insecta: Coleoptera asociadas con agaves (Asparagaceae: Agavoideae Mexican species of Curculionidae (Insecta: Coleoptera associated to agaves (Asparagaceae: Agavoideae

    Directory of Open Access Journals (Sweden)

    Alberto Romo

    2012-12-01

    Full Text Available Se estudiaron las especies de picudos o gorgojos (Coleoptera: Curculionidae asociadas con agaves (Asparagaceae: Agavoideae en México. Se registraron 5 especies asociadas con especies de Agave, Furcraea, Hesperoyucca, Polianthes y Yucca; de éstas, 4 pertenecen a la subfamilia Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis y Cactophagus spinolae y 1 a la Baridinae (Peltophorus polymitus. Se presentan diagnosis, ilustraciones y una clave para la identificación de las 5 especies de curculiónidos asociados con agaves. Se establecen las siguientes sinonimias: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; y Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891 y Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.Weevils (Coleoptera: Curculionidae associated with agaves (Asparagaceae: Agavoideae from Mexico are analyzed. Five species were recorded associated with species of Agave, Furcraea, Hesperoyucca, Polianthes, and Yucca. Four of these species belong to the subfamily Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis, and Cactophagus spinolae and 1 belongs to the subfamily Baridinae (Peltophorus polymitus. Diagnoses, illustrations and a key are presented for identifying the 5 species of weevils found on agaves. The following synonymies are established: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; and Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891, and Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.

  7. From forest to plantation? Obscure papers reveal alternate host plants for the coffee berry borer, Hypothenemus hampei (Coleoptera: Curculionidae)

    Science.gov (United States)

    The coffee berry borer, Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae), is the most devastating insect pest of coffee throughout the world. The insect is endemic to Africa but can now be found throughout nearly all coffee producing countries. One area of the basic biology of the insec...

  8. First record of the agave snout weevil, Scyphophorus acupunctatusGyllenhal (Coleoptera: Curculionidae: Dryophthorinae), in Puerto Rico

    OpenAIRE

    Setliff, Gregory P.; Anderson, Jesse A.

    2011-01-01

    The agave snout weevil, Scyphophorus acupunctatus Gyllenhal (Coleoptera: Curculionidae: Dryophthorinae), is reported from Puerto Rico for the first time. It was collected on feral sisal, Agave sisalana Perrine (Agavaceae), in the Guánica Dry Forest Reserve in the southwestern part of the island.

  9. Redescription of the Hispaniolan ladybird genus Bura Mulsant (Coleoptera: Coccinellidae) and justification for its transfer from Coccidulinae to Sticholotidinae

    Science.gov (United States)

    In the current work, we discuss the features of Bura (Coleoptera: Coccinellidae) that justify its transfer from Coccidulinae to Sticholotidinae, speculate on circumstances that led to its prior misclassification, and highlight current problems in the delineation of the afforementioned lady beetle su...

  10. A new genus for Drepanocanthoides larreae (Horn, 1887) and description of a new congeneric Mexican species (Coleoptera: Scarabaeidae: Aphodiinae)

    OpenAIRE

    Marco DELLACASA; Dellacasa, Giovanni; Gordon, Robert D.

    2016-01-01

    Drepanocanthoides larreae (Horn, 1887) (Coleoptera: Scarabaeidae: Aphodiinae) is transferred to the new genus Hornosus and the new species Hornosus turnbowi from Mexico (Nuevo León) is described. A complete set of fi gures is supplied for the taxa dealt with herein.

  11. Saprovisca sarangay, new species, a second species in an unusual Philippine genus of Eupariini (Coleoptera: Scarabaeidae: Aphodiinae)

    OpenAIRE

    Skelley, Paul E.

    2016-01-01

    A new species of Saprovisca Stebnicka (Coleoptera: Scarabaeidae) is described from the Philippines. This new species possesses sexual dimorphism in the form of clypeal horns in males which also show major and minor allometry, both are conditions rare in the Aphodiinae.

  12. Agrilus rubensteini, a new species from the Philippines related to the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae)

    Science.gov (United States)

    A new species from the Philippines closely related to the emerald ash borer, Agrilus planipennis Fairmaire, 1888 (Coleoptera: Buprestidae) is described: Agrilus rubensteini Chamorro & Jendek, new species. This is the first species in the A. cyaneoniger species-group recorded for the Philippines. Agr...

  13. Upper lethal temperature limits of the common furniture beetle Anobium punctatum (Coleoptera: Anobiidae)

    DEFF Research Database (Denmark)

    Hansen, Lise Stengård; Jensen, Karl-Martin Vagn

    The susceptibility of the egg, larval and adult stages of Anobium punctatum De Geer (Coleoptera: Anobiidae) to heat (46-54°C, 25-30% RH) was investigated. The larval stage was found to be most tolerant to heat. Very short exposure (5 min) of the larvae to temperatures of 52°C and above led to 100...

  14. Records of unsuccessful attack by Anoplophora glabripennis (Coleoptera: Cerambycidae) on broadleaf trees of questionable suitability in Ontario

    Science.gov (United States)

    Discovery of the non-native Anoplophora glabripennis Motschulsky (Coleoptera: Cerambycidae) in Ontario, Canada, in 2003 led to the implementation of an eradication program. The plan consisted of removing all infested trees and all trees within 400 m of an infested tree belonging to a genus consider...

  15. Seasonal flight activity and distribution of metallic woodboring beetles (Coleoptera: Buprestidae) collected in North Carolina and Tennessee

    Science.gov (United States)

    Metallic wood boring insects (Coleoptera: Buprestidae) species are responsible for high levels of host plant injury to deciduous shade and flowering trees in commercial nurseries, urban forests, and managed landscapes. Ornamental plant producers in the southeastern U.S. have ranked borers, includin...

  16. Resistance in Cultivated Sunflower Germplasm to the Red Sunflower Seed Weevil (Coleoptera: Curculionidae) in the Northern Great Plains

    Science.gov (United States)

    A 6-year field study evaluated 52 sunflower, Helianthus annuus L., accessions, 20 breeding lines, and 9 interspecific crosses for resistance to infestation by naturally occurring populations of the red sunflower seed weevil, Smicronyx fulvus LeConte (Coleoptera: Curculionidae). Germplasm with potent...

  17. Introduction and Recovery of Delphastus catalinae (Coleoptera: Coccinellidae) as a predator of Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt

    Science.gov (United States)

    Bemisia tabaci (Gennadius) (Hemiptera: Alyerodidae) is an important pest of many crops on a global scale. The use of biological control organisms such as coccinelid predators can help manage this pest. Delphastus catalinae (Horn) (Coleoptera: Coccinelidae) is an obligate predator of whiteflies, in...

  18. Quantitative relationship between potato tuber damage and counts of Pacific Coast wireworm (Coleoptera: Elateridae) in baits: seasonal effects.

    Science.gov (United States)

    Plots were baited with rolled oats in spring to assess the relationship between counts of Pacific coast wireworm, Limonius canus (Coleoptera: Elateridae) and damage to potato tubers. Baiting was done at 7 intervals beginning before planting of potatoes and ending following germination. Injury (per...

  19. Self-selection of two diet components by Tennebrio molitor (Coleoptera: Tenebrionidae) larvae and its impact on fitness

    Science.gov (United States)

    We studied the ability of Tenebrio molitor L. (Coleoptera: Tenebrionidae) to self-select optimal ratios of two dietary components to approach nutritional balance and maximum fitness. Life table analysis was used to determine the fitness of T. molitor developing in diet mixtures comprised of four dif...

  20. Novos táxons em Acanthocinini sul-americanos (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Marcela L. Monné

    2012-09-01

    Full Text Available Novos táxons em Acanthocinini sul-americanos (Coleoptera, Cerambycidae. São descritos e figurados os novos táxons: Trichonyssodrys nessimiani sp. nov. do Brasil (Bahia a Santa Catarina, Amniscites tavakiliani sp. nov. da Guiana Francesa and A. amboroensis sp. nov. da Bolívia (Cochabamba e Santa Cruz; Trichonius inusitatus sp. nov. e Lophopoenopsis itatiaiensis sp. nov. do Brasil (Rio de Janeiro e Luteolepturges gen. nov., espécie tipo L. galbus sp. nov., do Brasil (Amazonas, Rondônia, Peru e Equador.

  1. Nothoprodontia, um novo gênero de Trachyderini (Coleoptera, Cerambycidae, Cerambycinae

    Directory of Open Access Journals (Sweden)

    Miguel A. Monné

    2004-06-01

    Full Text Available Nothoprodontia gen. nov. e sua espécie-tipo, N. boliviana sp. nov., são descritos da Bolívia (Cochabamba. O novo gênero é comparado com Prodontia Audinet-Serville, 1834, Eriphus Audinet-Serville, 1834, and Athetesis Bates, 1870.Nothoprodontia, a new genus of Trachyderini (Coleoptera, Cerambycidae, Cerambycinae. Nothoprodontia gen. nov. and its type-species, N. boliviana sp. nov., are described from Bolívia (Cochabamba. Comparative notes with Prodontia Audinet-Serville, 1834, Eriphus Audinet-Serville, 1834, and Athetesis Bates, 1870 are also given.

  2. Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico

    Directory of Open Access Journals (Sweden)

    Sonia Maria Noemberg Lazzari

    2006-06-01

    Full Text Available Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico. O objetivo desta pesquisa foi avaliar o efeito do resfriamento artificial de grãos de arroz para o controle de coleópteros-praga. O ar frio foi insuflado pelo sistema de aeração em um silo metálico com arroz-em-casca. A avaliação do tratamento foi feita quinzenalmente usando armadilhas caladores. As espécies de Coleoptera capturadas foram: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16,5% e Sitophilus spp. (0,5%. Aos 28 dias, a temperatura média da massa de grãos era de 15ºC, e o número médio de insetos havia diminuído 76,8%. A aplicação de ar frio manteve as populações sob controle por aproximadamente 60 dias. Os resultados do monitoramento dos insetos e da temperatura indicaram que um novo ciclo de ar frio deveria ser aplicado nesse período para manter as populações sob controle. Também o manejo adequado da massa de grãos faz-se necessário para garantir resultados satisfatórios do resfriamento artificial.Artificial chilling to control Coleoptera in paddy rice stored in metallic silo. The objective of this research was to evaluate the effect of artificial chilling for the control of coleopterans in stored paddy rice. The cold air was insufflated through the aeration system of a metallic silo with paddy rice. Evaluation of insect number was made every 15 days using probe traps. The species of Coleoptera captured were: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16.5% and Sitophilus spp. (0.5%. By the 28th day the average temperature of the grain mass was 15ºC, and the mean number of insects decreased 76.8%. The cold air application kept the insect populations under control for approximately 60 days. The results of temperature and insect monitoring indicated that a new cycle of cold air should be applied by that time to keep the populations under

  3. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-09-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  4. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-06-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  5. Invertebrate fauna (Coleoptera, Collembola, Diplopoda, Isopoda collected in the karst areas of the Aninei - Locvei Mountains

    Directory of Open Access Journals (Sweden)

    Andrei Giurginca

    2011-08-01

    Full Text Available The authors identified 132 species of invertebrates (14 Oniscidea, 25 Diplopoda,31 Collembola and 62 Coleoptera recently sampled (2001–2006 from the soil and subterranean (MSS and caves environments from the Banat Mountains. Some new,rare and endemic species are discussed. The seasonal changes of the species diversity in the superficial subterranean environments at 0.5 to 1 m in depth are for the first time presented for the Reşiţa – Moldova Nouă synclinorium. The characteristic and preferential species for the mesovoid shallow substratum (MSS, belonging to the analyzed taxa, are identified.

  6. A review of the natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries.

    Science.gov (United States)

    Di Iorio, Osvaldo

    2014-01-01

    A compilation of the known natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries is provided. Food items of adult Cetoniinae include pollen and/or nectar (flower visitors), sap and/or slime flux, ripened fruits on plants, green tissues and leaves, and honey. Of the 36 species of Cetoniinae from Argentina, food items are known only for 11 species (30.5%). Attraction to light and bait-traps, adult activity periods, vertebrate predators, and the occurrence in bird nests are presented and discussed. Other insects that share the same food sources and bait-traps with Cetoniinae are mentioned. PMID:24869870

  7. Description of the third instar larvae of five species of Cyclocephala (Coleoptera, Melolonthidae, Dynastinae from Mexico

    Directory of Open Access Journals (Sweden)

    Miguel A. Morón

    2014-09-01

    Full Text Available Description of the third instar larvae of five species of Cyclocephala (Coleoptera, Melolonthidae, Dynastinae from Mexico. Larvae of four species of Cyclocephala are described for the first time based on specimens collected in Mexican localities: C. barrerai Martínez, 1969 from Puebla, C. sinaloae Howden & Endrödi, 1966 from Sinaloa, C. fasciolata Bates, 1888 from Veracruz, and C. jalapensis Casey, 1915 from Hidalgo. Larva of C. lunulata Burmeister, 1847, is redescribed based on specimens from the Mexican states of Morelos, Puebla, and Veracruz. Diagnostic structures are illustrated and the differences and similarities of each species with other previously described larvae of the genus are commented.

  8. Espécies de Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini ocorrentes na Bolívia

    Directory of Open Access Journals (Sweden)

    Maria Helena M. Galileo

    2013-03-01

    Full Text Available Species of Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini occurring in Bolivia. The genus Gorybia (Cerambycinae, Piezocerini consists of 45 described species with seven species recorded from Bolivia. Nine new species are described herein from Bolivia: G. abnormalis sp. nov.; G. alveolata sp. nov.; G. asyka sp. nov.; G. florida sp. nov.; G. inarmata sp. nov.; G. longithorax sp. nov.; G. guenda sp. nov.; G. tuberosa sp. nov. and G. wappesi sp. nov. A key to the species now known to occur in Bolivia is included.

  9. On the family- and genus-series nomina in Gyrinidae Latreille, 1810 (Coleoptera, Adephaga).

    Science.gov (United States)

    Gustafson, Grey T; Miller, Kelly B

    2013-01-01

    All available genus- and family- group nomina for the Gyrinidae (Coleoptera: Adephaga) are listed along with original citation, original and current status, type nominal taxon with method of designation, and known synonymies and incorrect subsequent spellings. The nomina included follow the most current classification. Discussion is provided clarifying numerous nomenclatural problems with original spellings, correct authorship and type designation. Dineutini Ochs, 1926 syn. nov. is found to be a junior homonym of Dineutini Desmarest, 1851, and Enhydrini Régimbart, 1882 syn. nov. and its justified emendation Enhydrusini (Anonymous 2012) are here synonymized with Dineutini Desmarest, 1851. PMID:25277555

  10. Gross anatomy of central nervous system in firefly, Pteroptyx tener (Coleoptera: Lampyridae)

    Science.gov (United States)

    Hudawiyah, Nur; Wahida, O. Nurul; Norela, S.

    2015-09-01

    This paper describes for the first time the organization and fine structure of the central nervous system (CNS) in the fireflies, Pteroptyx tener (Coleoptera: Lampyridae). The morphology of the CNS was examined by using Carl Zeiss AxioScope A1 photomicroscope with iSolution Lite software. Some specific structural features such as the localization of protocerebrum, deutocerebrum and tritocerebrum in the brain region were analyzed. Other than that, the nerve cord and its peripheral structure were also analyzed. This study suggests that, there is a very obvious difference between male and female central nervous system which illustrates that they may differ in function in controlling physiological and behavioral activities.

  11. Soil and saproxylic species (Coleoptera, Collembola, Araneae in primeval forests from the northern part of South-Easthern Carpathians

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    Eugen Nitu

    2009-11-01

    Full Text Available In 2006-2007 we carried out faunal investigations in the vernal, estival and autumnal seasons in the scientific reserve "Codrul Secular Giumalãu" using quantitative sampling methods. We identified 189 species of Coleoptera, 70 of Collembola and 20 of Araneae. Of these, 11 phytophagous, 18 myceto/xylo-mycetophagous,9 mixophagous, 18 xylo- and cambio-xylemophagous, 38 saproxylophagous,125 (55 Coleoptera, 70 Collembola detritivorous (sapro-, copro- andnecrophagous, 60 (40 Coleoptera, 20 Aranea predators/parasitoids. Hymenaphorura polonica Pomorski, 1990 (Collembola, and Leiodes rhaeticus Erichson, 1845 (Coleoptera, Leiodidae, are recorded for the first time in the Romanian fauna. The rare species and characteristic species for the old primeval spruce forests are analysed for each studied taxonomic group. The species richness and faunal diversity from the Giumalãu primeval spruce forest are compared with those of other very well preserved forests from the Carpathians scientific reserves (Codrul Secular Slãtioara,Pietrosul Rodnei. The species abundances were used to compute the similarity indexes between the sampled sectors of forest and to perform Cluster Analysis. We observed that the dead wood in the 2nd-6th phases of decomposition has a great influence not only on the saproxylic species but also on the soil fauna like ground beetles(Carabidae that use the logs as ecologic microrefuges (winter refugees or diurnal refugees. The structure of the soil fauna is influenced by wood extraction from the forest ecosystem or by natural perturbations, this consisting in the appearance of opportunistic species as Orchesella pontica (Collembola and in decreasing ofspecies richness of Carabidae (Coleoptera.

  12. The hydraulic mechanism in the hind wing veins of Cybister japonicus Sharp (order: Coleoptera).

    Science.gov (United States)

    Sun, Jiyu; Wu, Wei; Ling, Mingze; Bhushan, Bharat; Tong, Jin

    2016-01-01

    The diving beetles (Dytiscidae, Coleoptera) are families of water beetles. When they see light, they fly to the light source directly from the water. Their hind wings are thin and fragile under the protection of their elytra (forewings). When the beetle is at rest the hind wings are folded over the abdomen of the beetle and when in flight they unfold to provide the necessary aerodynamic forces. In this paper, the unfolding process of the hind wing of Cybister japonicus Sharp (order: Coleoptera) was investigated. The motion characteristics of the blood in the veins of the structure system show that the veins have microfluidic control over the hydraulic mechanism of the unfolding process. A model is established, and the hind wing extending process is simulated. The blood flow and pressure changes are discussed. The driving mechanism for hydraulic control of the folding and unfolding actions of beetle hind wings is put forward. This can assist the design of new deployable micro air vehicles and bioinspired deployable systems. PMID:27547607

  13. Dung beetles (Coleoptera, Scarabaeinae attracted to sheep dung in exotic pastures

    Directory of Open Access Journals (Sweden)

    César Murilo de Albuquerque Correa

    2013-03-01

    Full Text Available Dung beetles (Coleoptera, Scarabaeinae attracted to sheep dung in exotic pastures. In this study we provide data on the abundance and richness of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae attracted to sheep dung in exotic pastures (Brachiaria spp.. In four areas of exotic pasture pitfall traps were installed and baited with fresh sheep dung for sampling of dung beetles. A total of 2,290 individuals were captured belonging to 16 species, 10 genera and five tribes of Scarabaeinae beetles. Trichillum externepunctatum Preudhomme de Borre, 1886 and Dichotomius bos (Blanchard, 1843 were dominant. The guild of dwellers was the most abundant in pastures. We demonstrate that dung beetles are attracted to sheep dung. Since the production of both cattle and sheep in the same area is common in tropical pasturelands, results obtained here highlight the need to investigate the actual role of dung sharing (cattle dung + sheep dung by dung beetles. It is also suggested that experiments be performed for evaluation of the ecological functions performed by dung beetles using sheep dung.

  14. Ophiostoma species (Ascomycetes: Ophiostomatales) associated with bark beetles (Coleoptera: Scolytinae) colonizing Pinus radiata in northern Spain.

    Science.gov (United States)

    Romón, Pedro; Zhou, XuDong; Iturrondobeitia, Juan Carlos; Wingfield, Michael J; Goldarazena, Arturo

    2007-06-01

    Bark beetles (Coleoptera: Scolytinae) are known to be associated with fungi, especially species of Ophiostoma sensu lato and Ceratocystis. However, very little is known about these fungi in Spain. In this study, we examined the fungi associated with 13 bark beetle species and one weevil (Coleoptera: Entiminae) infesting Pinus radiata in the Basque Country of northern Spain. This study included an examination of 1323 bark beetles or their galleries in P. radiata. Isolations yielded a total of 920 cultures, which included 16 species of Ophiostoma sensu lato or their asexual states. These 16 species included 69 associations between fungi and bark beetles and weevils that have not previously been recorded. The most commonly encountered fungal associates of the bark beetles were Ophiostoma ips, Leptographium guttulatum, Ophiostoma stenoceras, and Ophiostoma piceae. In most cases, the niche of colonization had a significant effect on the abundance and composition of colonizing fungi. This confirms that resource overlap between species is reduced by partial spatial segregation. Interaction between niche and time seldom had a significant effect, which suggests that spatial colonization patterns are rarely flexible throughout timber degradation. The differences in common associates among the bark beetle species could be linked to the different niches that these beetles occupy. PMID:17668036

  15. Variation in susceptibility of laboratory and field strains of three stored-grain insect species to beta-cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to concrete surfaces

    Science.gov (United States)

    The efficacy of beta-cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to clean, concrete floors of empty bins prior to grain storage against field strains of stored-grain insects is unknown. We exposed adults of 16 strains of the red flour beetle, Tribolium castaneum (Herbst); 8 strains ...

  16. Effects of Short Exposures to Spinosad-Treated Wheat or Maize on Four Stored-Grain Insects

    Science.gov (United States)

    The effect of short exposures to spinosad-treated wheat and maize was evaluated against adults of four stored-product insects: the lesser grain borer, Rhyzopertha dominica (F.), the rice weevil, Sitophilus oryzae (L.), the red flour beetle, Tribolium castaneum (Herbst), and the psocid Lepinotus reti...

  17. Methodology for evaluating the insect growth regulator (IGR) methoprene incorporated into packaging films

    Science.gov (United States)

    The insect growth regulator methoprene has been impregnated onto various packaging materials to control stored product insects, and is labeled for use in this manner in the United States. Different methodologies were utilized to evaluate efficacy towards Tribolium castaneum (Herbst), the red flour b...

  18. ESCARABAJOS TIGRE (COLEOPTERA: CICINDELIDAE DEL MUSEO ENTOMOLÓGICO FRANCISCO LUÍS GALLEGO: NUEVOS REGISTROS PARA DEPARTAMENTOS DE COLOMBIA TIGER BEETLES (COLEOPTERA: CICINDELIDAE AT THE ENTOMOLOGICAL MUSEUM FRANCISCO LUÍS GALLEGO: NEW RECORDS

    Directory of Open Access Journals (Sweden)

    Manuel Alejandro Ramírez Mora

    2008-06-01

    Full Text Available Se registran por primera vez las especies de escarabajos tigre (Coleoptera: Cicindelidae presentes en el Museo Entomológico Francisco Luis Gallego (MEFLG. Se identificaron 167 especimenes distribuidos en ocho géneros y 27 especies, se reportan 24 nuevos registros para diferentes departamentos de Colombia. Se señalan aspectos importantes de la taxonomía y sistemática del grupo. Además, se presentan comentarios biológicos y de distribución de las especies.Tiger beetles (Coleoptera: Cicindelidae species at the Museo Entomológico Francisco Luis Gallego (MEFLG are registered by first time. 167 specimens in 8 genus and 27 species were identified, 24 new records for different Colombian states are reported. Some important aspects of the group’s taxonomy and systematic are pointed. Additionally, species’ biological and distributional comments are presented.

  19. Especies de Megacerus (Coleoptera: Bruchidae y sus plantas hospederas en Yucatán Megacerus species (Coleoptera: Bruchidae and their host plants in Yucatán

    Directory of Open Access Journals (Sweden)

    Enrique Reyes

    2009-12-01

    Full Text Available Se registra la incidencia de Megacerus (Coleoptera: Bruchidae en semillas de 7 especies de Convolvulaceae de 6 sitios al norte de Yucatán. Las semillas de Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia y M. cissoides recolectadas en etapa de dispersión fueron colocadas en sobres limpios de papel y puestas en condiciones de laboratorio hasta la emergencia de los brúquidos. Se obtuvieron 1111 coleópteros pertenecientes a 5 especies: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, y M. (Serratibruchus cubiciformis. Las semillas de I. crinicalyx, M. aegyptia y M. cissoides fueron infestadas principalmente por M. (M. porosus, las de I. hederifolia e I. triloba por M. (M. cubiculus y las de I. nil por M. (M. tricolor. Todos los individuos de Megacerus (M. sp., fueron encontrados en semillas de J. penthanta. En el nivel génerico de hospedero se encontró que las semillas de Ipomoea fueron infestadas principalmente por M. (M. cubiculus y las de Merremia por M. (M. porosus. Estos resultados amplían el rango de plantas hospederas registrado para las especies de Megacerus y resaltan la estrecha asociación que existe entre un linaje de depredadores de semillas Bruchidae y plantas de la familia Convolvulaceae.Seed-attacking by Megacerus (Coleoptera: Bruchidae is reported for 7 Convolvulaceae species from 6 sites at the North of Yucatan. Seeds of Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia and M. cissoides were collected and placed in clean envelopes under laboratory conditions to collect all bruchids emerged from seeds. A total of 1111 coleopterans were hatched from seeds, and 5 taxa were identify: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, and M. (Serratibruchus cubiciformis. Seeds of I. crinicalyx, M. aegyptia and M. cissoides were attacked mainly by M. (M. porosus; I

  20. Longhorned Beetles Collection of the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey (Coleoptera,Cerambycidae)

    OpenAIRE

    ÖZDİKMEN, Hüseyin; ŞAHİN, Özlem

    2010-01-01

    In the present paper, specimens of Cerambycidae were examined in the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey. As a result of identification of these specimens, thirty-eight species and two subspecies belonging to twenty-eight genera of five subfamilies were determined. With this paper, new faunistic data and some zoogeographical evaluations were presented on longhorned beetles fauna (Coleoptera, Cerambycidae) of Turkey. The faunistic data in the pr...

  1. Synergistic effects of chlorpyrifos with piperonyl butoxide (pbo) against the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

    OpenAIRE

    Akter Mst Yeasmin; Talukdar Muhammad Waliullah; ASM Shafiqur Rahman

    2014-01-01

    Objective: To investigate the co-toxicity and co-efficient activity of Chlorpyrifos (Dursban 20EC), an organophosphate and Piperonyl butoxide (PBO) against the lesser meal worm Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) (A. diaperinus). Methods: The repellent activity was carried out by the residual film assay technique. Statistically the dose mortality relationship was expressed as a median lethal dose (LD50) by the probit analysis. The regression lines and isoboles were ...

  2. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    OpenAIRE

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineens...

  3. Natural history of Belonuchus Nordmann spp. and allies (Coleoptera: Staphylinidae) in Heliconia L. (Zingiberales: Heliconiaceae) flower bracts

    OpenAIRE

    Frank, J. Howard; Barrera, Roberto

    2010-01-01

    Adults, and in some species larvae, of several members of Belonuchus Nordmann (Coleoptera: Staphylinidae: Staphylininae) and a few related genera seem to be to various degrees consistently associated with flower bracts of the genus Heliconia (Zingiberales: Heliconiaceae). They are predators and eat various dipterous and lepidopterous larvae in that habitat. Adults of at least Belonuchus cephalotes (Sharp) and Odontolinus fasciatus Sharp are able to immerse completely in water to capture larva...

  4. Uji Efektifitas Beberapa Insektisida Nabati Terhadap Mortalitas Hama Sitophilus zeamais Motsch. (Coleoptera; Curculionidae) Pada Benih Jagung (Zea mays)

    OpenAIRE

    Subhan, Fadillah

    2011-01-01

    Fadillah Subhan, "Test Effectiveness of Some Insecticides Against Plant Pests Sitophilus zeamais Motsch Mortality. (Coleoptera: Curculionidae) In the Seed of Corn (Zea mays). "It was under supervised by Yuswani Pangestiningsih and Fatimah Zahara. S. zeamais is a pest of corn in storage that causes substantial yield losses, one of which effective control is the use of botanical insecticides. Testing the effectiveness of some botanical insecticides on mortality of S. zeamais on maize seed plant...

  5. A review of Elocomosta Hansen with a description of a new species with reduced eyes from China (Coleoptera, Hydrophilidae, Sphaeridiinae)

    Science.gov (United States)

    Lin, Renchao; Jia, Fenglong; Fikáček, Martin

    2016-01-01

    Abstract A new species of the genus Elocomosta Hansen, 1989 (Coleoptera: Hydrophilidae: Sphaeridiinae: Coelostomatini), Elocomosta lilizheni sp. n., is described from Guangxi Province, China. It is compared in detail with the only other known species of the genus, Elocomosta nigra Hansen, 1989 from Borneo, and the genus is diagnosed from the remaining coelostomatine genera. The new species is unusual among Hydrophilidae by having extremely reduced eyes. PMID:27551232

  6. Diversity and phylogenetic analysis of endosymbiotic bacteria of the date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae)

    OpenAIRE

    El-Sayed, Wael S.; Ibrahim, Reda A

    2015-01-01

    Background The date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in food digestion and insecticide resistance mechanisms, and therefore are essential to their host insect. Identification of these bacteria in their insect host can lead to developme...

  7. First record of Rhoptrocentrus piceus Marshall (Hymenoptera, Braconidae, Doryctinae as parasitoid of Psacothea hilaris hilaris (Pascoe (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Augusto Loni

    2015-02-01

    Full Text Available The species Rhoptrocentrus piceus Marshall (Hymenoptera: Braconidae was reared from the larvae of the xylophagous beetle Psacothea hilaris hilaris (Pascoe (Coleoptera: Cerambycidae, an exotic pest of Ficus and Morus species native to eastern Asia. It was recorded in the north of Italy in September 2005. This discovery is the first report of this species as parasitoids of the yellow spotted longicorn beetle all over the world.

  8. First record of Rhoptrocentrus piceus Marshall ( Hymenoptera , Braconidae , Doryctinae ) as parasitoid of Psacothea hilaris hilaris (Pascoe) ( Coleoptera , Cerambycidae )

    OpenAIRE

    Augusto Loni; Costanza Jucker; Sergey Belokobylskij; Daniela Lupi

    2015-01-01

    Abstract The species Rhoptrocentrus piceus Marshall ( Hymenoptera : Braconidae ) was reared from the larvae of the xylophagous beetle Psacothea hilaris hilaris (Pascoe) ( Coleoptera : Cerambycidae ), an exotic pest of Ficus and Morus species native to eastern Asia. It was recorded in the north of Italy in September 2005. This discovery is the first report of this species as parasitoids of the yellow spotted longicorn beetle all over the world.

  9. Ground beetle (Coleoptera: Carabidae) assemblages in narrow hedgerows in a Danish agricultural landscape

    DEFF Research Database (Denmark)

    Lövei, G. L.; Magura, T.

    2015-01-01

    The role of hedgerows in supporting ground beetles (Coleoptera: Carabidae) in a Danish agricultural landscape was examined. Nine old, well established single-row hedges were selected for the study, three each of a native species (hawthorn, Crataegus monogyna), a non-native deciduous one (rowan...... beetle assemblages. The number of ground beetle individuals and species were significantly the highest in the hawthorn hedges and significantly decreased from the hedges with rowan toward the spruce hedges. The elevated number of ground beetle individuals and species in the hawthorn hedges were due...... to the forest specialist species, as the number of forest specialist ground beetle individuals and species were significantly higher in the hawthorn hedges compared to the hedges with rowan and spruce. Differences in the number of the grassland and the cropland specialist ground beetle individuals and species...

  10. A importância forense de Oxelytrum discicolle (Brullé, 1840) (Coleoptera, Silphidae)

    OpenAIRE

    Camila Kraiczyi Kotzko; V. Costa-Silva; E. G. Silva

    2015-01-01

    Oxelytrum discicolle Brullé, 1840 é a espécie mais coletada de Silphidae (Coleoptera) na América Latina, constituindo um indicador forense na região Neotropical. O objetivo do trabalho é reunir informações sobre biologia e distribuição de O. discicolle, além de ferramentas para utilização desse besouro como indicador forense em casos de morte de humanos. O. discicolle é um besouro de grande porte, de cor preta com um colar vermelho. Larvas da espécie são necrófagas, já os adultos são onívoros...

  11. The first complete mitochondrial genome of stag beetle from China, Prosopocoilus gracilis (Coleoptera, Lucanidae).

    Science.gov (United States)

    Wu, Yuan-Yuan; Cao, Yu-Yan; Fang, Jie; Wan, Xia

    2016-07-01

    The complete mitochondrial genome (mitogenome) of Prosopocoilus gracilis (Coleoptera: Lucanidae) that is endemic to Southern China is determined. The circular genome is 736 bp in length and comprises 13 protein-coding genes, 22 tRNA, 2 rRNA genes and a control region. Gene order is identical to that of the putative ancestral arrangement of insects. The nucleotide composition of heavy strand is A (36.6%), C (22.6%), T (29.5%) and G (11.3%). All protein-coding genes start with a typical ATN codon except for the gene COI that uses AAC as the start codon. tRNA-Ser (AGN) uses the anticodon UCU instead of the commonly used GCU. Both maximum likelihood and Bayesian analyses support the monophyly of Lucanidae and the sister relationship of Nigidionus and the remaining sampled genera. Two species of Prosopocoilus were not recovered as a monophyletic group. PMID:26024142

  12. Estudos bioecológicos de Syphraea uberabensis (Coleoptera: Chrysomelidae Bechyné 1956

    Directory of Open Access Journals (Sweden)

    Charles Wikler

    2005-06-01

    Full Text Available Tibouchina herbacea (DC. Cog. é uma planta ornamental introduzida propositadamente no arquipélago do Havaí e, devido à ausência de inimigos naturais e condições edafo-climáticas adequadas, está se dispersando rapidamente pelas florestas nativas e regiões úmidas das principais ilhas havaianas. Por ser originária do Brasil, foram realizadas diversas viagens exploratórias em busca de por inimigos naturais específicos que pudessem controlar esta planta. Dentre os agentes selecionados com impacto e potencial de especificidade à T. herbacea encontra-se Syphraea uberabensis (Bechyne, 1955 (Coleoptera: Chrysomelidae. Este trabalho visa estudos sobre a biologia, ecologia e especificidade deste inseto e também uma avaliação criteriosa dos possíveis impactos causados por este inimigo natural na população da planta.

  13. The mortality of Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae induced by powdered plants

    Directory of Open Access Journals (Sweden)

    Kłyś Małgorzata

    2015-01-01

    Full Text Available The aim of this study was to investigate whether powdered plants of different species namely: peppermint Mentha piperita (L. (Lamiaceae, wormwood Artemisia absinthium (L. (Asteraceae, common sage Salvia officinalis (L. (Lamiaceae, allspice Pimenta dioica (Linnaeus et Merrill (Myrtaceae and common garlic Allium sativum (L. (Amaryllidaceae, added to semolina using concentrations of 1.23, 3.61, and 5.88%, influence the mortality rate in the saw-toothed grain beetle Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae. Experiments were conducted in a laboratory at 28°C and relative humidity 60±5%. At the concentration of 1.23%, allspice seeds caused the highest mortality amongst the saw-toothed grain beetle. When concentrations of 3.61 and 5.88% were used, sage, peppermint and wormwood caused the highest statistically significant mortality of O. surinamensis

  14. Communication disruption using synthetic sex pheromone for control of sugarcane wireworm. Melanotus okinawensis Ohira (Coleoptera: Elateridae)

    International Nuclear Information System (INIS)

    We tested communication disruption through the use of a synthetic sex pheromone from mid February to early June 2001 at the cultivating land on Minami-Daito Island in order to control the sugarcane wireworm Melanotus okinawensis Ohira (Coleoptera: Elateridae). Although very few males were caught in monitoring traps except a few traps set in a treated area, a large number were caught in traps set in an untreated Japanese pampas grass field. This indicates that communication disruption was effective throughout the period in the treated area. Females collected from treated area of Minami-Daito Island, mating rates that were lower than those of females in the untreated areas on Miyagi Island. We therefore concluded that a communication disruption system is an effective tool for the sugarcane wireworm management. (author)

  15. What do we know about winter active ground beetles (Coleoptera, Carabidae in Central and Northern Europe?

    Directory of Open Access Journals (Sweden)

    Radomir Jaskula

    2011-05-01

    Full Text Available This paper summarizes the current knowledge on winter active Carabidae in Central and Northern Europe. In total 73 winter active species are listed, based on literature and own observations. Ground beetles are among the three most numerous Coleoptera families active during the autumn to spring period. The winter community of Carabidae is composed both of larvae (mainly autumn breeding species and adults, as well as of epigeic species and those inhabiting tree trunks. Supranivean fauna is characterized by lower species diversity than the subnivean fauna. The activity of ground beetles decreases in late autumn, is lowest during mid-winter and increases in early spring. Carabidae are noted as an important food source in the diet of insectivorous mammals. They are also predators, hunting small winter active invertebrates.

  16. Synthesis, antifeedant activity against Coleoptera and 3D QSAR study of alpha-asarone derivatives.

    Science.gov (United States)

    Łozowicka, B; Kaczyński, P; Magdziarz, T; Dubis, A T

    2014-01-01

    For the first time, a set of 56 compounds representing structural derivatives of naturally occurring alpha-asarone as an antifeedants against stored product pests Sitophilus granarius L., Trogoderma granarium Ev., and Tribolium confusum Duv., were subjected to the 3D QSAR studies. Three-dimensional quantitative structure-activity relationships (3D-QSAR) for 56 compounds, including 15 newly synthesized, were performed using comparative molecular field analysis s-CoMFA and SOM-CoMSA techniques. QSAR was conducted based on a combination of biological activity (against Coleoptera larvae and beetles) and various geometrical, topological, quantum-mechanical, electronic, and chromatographic descriptors. The CoMSA formalism coupled with IVE (CoMSA-IVE) allowed us to obtain highly predictive models for Trogoderma granarium Ev. larvae. We have found that this novel method indicates a clear molecular basis for activity and lipophilicity. This investigation will facilitate optimization of the design of new potential antifeedants. PMID:24601760

  17. Taxonomy of Colophon Gray (Coleoptera: Lucanidae): new species and a status change.

    Science.gov (United States)

    Jacobs, Carmen T; Scholtz, Clarke H; Strümpher, Werner P

    2015-01-01

    Three new species of the Cape high-mountain stag beetle genus, Colophon Gray (Coleoptera: Lucanidae), from South Africa are described. They are C. deschodti new species, C. switalae new species, and C. struempheri new species. The new taxa fall within a species complex of geographically disjunct entities related to Colophon stokoei Barnard. Furthermore, the mitochondrial COI gene shows a high degree of sequence divergence, with pairwise genetic distances between the species ranging between 7.4-10.7%. The new species are illustrated by photographs. Colophon eastmani nagaii Mizukami is raised to species level on the basis of geographic range and molecular differences between it and the nominate subspecies. This brings the total number of described species in the genus to 21. An updated checklist of the South African species of Colophon is also provided. PMID:26701471

  18. Carpophilus zeaphilus, a new sap beetle species acclimatized in Italy (Coleoptera: Nitidulidae

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    Paolo Audisio

    2015-06-01

    Full Text Available Carpophilus zeaphilus Dobson, 1969 (Coleoptera, Nitidulidae, Carpophilinae is an Afrotropical species that has become widespread in Sub-Saharan Africa, the Arabian Peninsula, and southern Mediterranean areas in recent years. The species was first recorded from Europe in Portugal and Spain nearly thirty years ago, and it was later intercepted in Sicily near Trapani in 1991. A few specimens of this species were collected in April, 2015 in a sparsely forested area near Rome, which suggests a recent acclimatization into peninsular Italy. Specimens were taken on flowering trees of Prunus spinosa L. (Rosaceae, an unusual occurrence for most introduced species of Carpophilinae that are normally associated with rotten fruit and other decomposing vegetal matter.

  19. A review of the weevil fauna (Coleoptera, Curculionoidea of Araucaria angustifolia (Bert. O. Kuntze (Araucariaceae in South Brazil

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    Roland Mecke

    2004-09-01

    Full Text Available The beetle superfamily Curculionoidea includes 43 species associated with Araucaria angustifolia trees in South Brazil. These weevil species belong to the families Nemonychidae (Brarus Kuschel, 1997, Rhynchitoplesius Voss, 1952, Brentidae (Taphroderes Schönherr, 1826 and Curculionidae, the latter including the subfamilies Curculioninae (Heilipodus Kuschel, 1955, Spermologus Schönherr, 1843, Cossoninae (Araucarius Kuschel, 1966, Eurycorynophorus Voss, 1964, Scolytinae (Ambrosiodmus Hopkins, 1915, Araptus Eichhoff, 1871, Cnesinus LeConte, 1868, Corthylus Erichson, 1836, Cryptocarenus Eggers, 1936, Hypothenemus Westwood, 1834, Monarthrum Kirsch, 1866, Pagiocerus Eichhoff, 1868, Phloeotribus Latreille, 1896, Pityophthorus Eichhoff, 1864, Xylechinosomus Schedl, 1963, Xyleborus Eichhoff, 1864, Xyleborinus Reitter, 1913 and Platypodinae (Cenocephalus Chapuis, 1865, Platypus Herbst, 1893, Tesserocerus Saunders, 1836. A checklist of all species including remarks on their life histories and taxonomic notes are presented. In addition, a key for the identification of adult Curculionoidea associated with Araucaria angustifolia to genus or species level is provided.A superfamília Curculionoidea compreende 43 espécies associadas à Araucaria angustifolia no sul do Brasil. As espécies destes gorgulhos pertencem às famílias Nemonychidae (Brarus Kuschel, 1997, Rhynchitoplesius Voss, 1952, Brentidae (Taphroderes Schönherr, 1826 e Curculionidae, (Curculioninae: Heilipodus Kuschel, 1955, Spermologus Schönherr, 1843; Cossoninae: Araucarius Kuschel, 1966, Eurycorynophorus Voss, 1964; Scolytinae: Ambrosiodmus Hopkins, 1915, Araptus Eichhoff, 1871, Cnesinus LeConte, 1868, Corthylus Erichson, 1836, Cryptocarenus Eggers, 1936, Hypothenemus Westwood, 1834, Monarthrum Kirsch, 1866, Pagiocerus Eichhoff, 1868, Phloeotribus Latreille, 1896, Pityophthorus Eichhoff, 1864, Xylechinosomus Schedl, 1963, Xyleborus Eichhoff, 1864, Xyleborinus Reitter, 1913; Platypodinae

  20. Diversidade de Carabidae (Coleoptera) Amostrados em Áreas de Reflorestamento de Mata Ciliar e Fragmento Florestal, no Estado do Paraná

    OpenAIRE

    Tamara Quinteiro; José Lopes; Ivan Martins

    2012-01-01

    Carabidae são Coleoptera com a maioria de suas espécies apresentando hábito alimentar predatório. O objetivo do presente trabalho foi estudar a diversidade e abundância da família Carabidae (Coleoptera) em áreas de fragmento florestal e em áreas de reflorestamento de mata ciliar, evidenciando a importância deste grupo como indicador da biodiversidade em relação ao sucesso do reflorestamento. Os besouros foram amostrados por meio de armadilhas de solo pitfall, em fragmentos florestais e reflor...

  1. Locomotor activity of Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae on Chilean sandy beaches Actividad locomotora de Phalerisida maculata Kulzer (Coleoptera, enebrionidae en playas arenosas chilenas

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    EDUARDO JARAMILLO

    2000-03-01

    Full Text Available The locomotor activity of the beetle Phalerisida maculata Kulzer (Coleoptera Tenebrionidae was studied on the surface of the substrate in two sandy beaches of the Chilean coast: one in north central (ca. 29ºS and the other in south central Chile (ca.39ºS. During the summer period of 1991 the circadian locomotor activity was studied in the southern beach, while during that of 1997 in both beaches. To analyze the activity, pitfall traps were used which were ordered along two transects extended between the upper beach and the resurgence zone. The traps were checked (i.e. collection of captured insects every two hours for a total period of 26 hours. The results showed that the adults of P. maculata were mostly active during the night hours, whereas the larvae were active during both, the day and night. Studies carried out in the beach located in south central Chile show that differences in the tidal range (neap vs. spring tides do not affect the activity patterns. During the locomotor activity, adult and larvae move to lower intertidal levels than those usually occupied while buried. Results of laboratory experiments using actographs under conditions of darkness and constant temperature, suggest that adults and larvae of P. maculata presented a circadian rhythm similar to that observed in the field experiments. It is concluded that P. maculata presents a behaviour that appear to be under control of an endogenous rhythm, without showing differences in the circadian rhythm of activity when beaches located at different latitudes are comparedSe estudió la actividad locomotriz del escarabajo Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae sobre la superficie del sustrato de dos playas arenosas de la costa de Chile: una en el centro norte (ca. 29ºS y otra en el centro sur (ca. 39ºS. Durante el período estival de 1991 se estudió la actividad locomotriz circadiana en la playa del centro sur y durante el de 1997 en ambas playas. Para analizar

  2. Nuevas citas de Coleoptera para la Argentina (Carabidae, Lucanidae, Scarabaeidae y Tenebrionidae New records of Coleoptera for Argentina (Carabidae, Lucanidae, Scarabeidae and Tenebrionidae

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    Sergio Roig-Juñent

    2004-12-01

    Full Text Available En enero de 2003, se realizó un viaje de campaña con el fin de recolectar materiales en áreas poco prospectadas de la estepa patagónica de Mendoza y Neuquén, Argentina, así como en ambientes de bosques de Nothofagus en la provincia de Neuquén. Las recolecciones se realizaron en forma manual, usando trampas con cebo y trampas de luz de mercurio. El material recolectado permite citar por primera vez para la Argentina los géneros Nothobroscus Roig-Juñent & Ball y Mimophilorizus Mateu (Carabidae, Callyntra Solier y Homocyrtus Reitter (Tenebrionidae. Además, se citan por primera vez para la Argentina las especies Cnemalobus sulciferus Philippi (Carabidae, Allidiostoma landbecki (Philippi, Macrodactylus chilensis Solier y Brachysternus marginatus (Germain (Scarabeidae y Pycnosiphorus philippii (Westwood (Lucanidae. Se provee una breve descripción de los ambientes en donde estas especies fueron encontradas.A short expedition to northern Patagonia was made in January 2003. The main goal of the trip was to survey the diversity of some families of Coleoptera in the insufficient explored steppes in Mendoza and Neuquén provinces, Argentina and Nothofagus forests of Neuquén province. The techniques included manual collection and the use of different kinds of traps, such as with bite or artificial light of mercury. The study of the material revealed four new genera records and four new species records for the country: Nothobroscus Roig-Juñent & Ball and Mimophilorizus Mateu (Carabidae, Callyntra Solier and Homocyrtus Reitter (Tenebrionidae are the new genus records. Cnemalobus sulciferus Philippi (Carabidae, Allidiostoma landbecki (Philippi, Macrodactylus chilensis Solier, and Brachysternus marginatus (Germain (Scarabeidae, and Pycnosiphorus philippii (Westwood (Lucanidae are the new species records. A brief description of the habitats where the species were collected is provided.

  3. Immunofluorescence localization and ultrastructure of Stewart’s wilt disease bacterium Pantoea stewartii in maize leaves and in its flea beetle vector Chaetocnema pulicaria (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Pantoea stewartii is the causal agent of Stewart's wilt of sweet corn, the most serious bacterial disease of sweet corn and maize in the North-Central and Eastern USA. P. stewartii is transmitted mainly by the corn flea beetle Chaetocnema pulicaria (Coleoptera: Chrysomelidae) and this bacterium is a...

  4. Preliminary evaluation of Delphastus catalinae (Coleoptera:Coccinellidae)as a predator of the ficus whitefly, Singhiella simplex (Singh) (Hemiptera:Aleyrodidae)

    Science.gov (United States)

    The predatory lady beetle Delphastus catalinae (Horn) (Coleoptera: Coccinellidae) was evaluated as a potential biological control agent against the ficus whitefly, Singhiella simplex (Singh) (Hemiptera: Aleyrodidae), a newly-invasive pest of ficus plants. Adult D. catalinae females were starved for ...

  5. Brood production by Xylosandrus germanus (Coleoptera: Curculionidae) and growth of its fungal symbiont on artificial diet based on sawdust of different species

    Science.gov (United States)

    The ambrosia beetle Xylosandrus germanus (Blanford) (Coleoptera: Curculionidae) is among the most important exotic pests of orchards and nurseries in the US. It attacks a wide range of hosts and is difficult to control using conventional insecticides. As part of our studies on the biology and cont...

  6. A new genus and species of myrmecophilous aphodiine beetle (Coleoptera, Scarabaeidae inhabiting the myrmecophytic epiphyte Platycerium sp. (Polypodiaceae in the Bornean rainforest canopy

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    Munetoshi Maruyama

    2010-01-01

    Full Text Available Pterobius itiokai Maruyama, gen. n. and sp. n., (Coleoptera: Scarabaeidae: Aphodiinae is described from Lambir Hills National Park, Borneo based on specimens collected from a Crematogaster difformis ant nest in the myrmecophytic epiphytic fern genus Platycerium. Pterobius belongs to the tribe Eupariini and is closely related to the Indo-Australian genus Cnematoplatys.

  7. Assembly and annotation of full mitochondrial genomes for the corn rootworm species, Diabrotica virgifera virgifera and D. barberi (Insecta: Coleoptera: Chrysomelidae), using Next Generation Sequence data

    Science.gov (United States)

    Complete mitochondrial genomes for two corn rootworm species, Diabrotica v. virgifera (16,747 bp) and D. barberi (16,632; Insecta: Coleoptera: Chrysomelidae), were assembled from Illumina HiSeq2000 read data. Annotation indicated that the order and orientation of 13 protein coding genes (PCGs), and...

  8. New records of Paracrias Ashmead (Hymenoptera, Eulophidae as parasitoids on weevil larvae (Coleoptera, Curculionidae in Brazil, with the description of a new species

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    Luciano Palmieri

    2013-09-01

    Full Text Available Paracrias strii Schauff, 1985 and P. ceratophaga Palmieri & Hansson sp. nov. are first record in Brazil and both are associated with Ceratopus Schoenherr larvae (Coleoptera, Curculionidae reared from syconia of two species of fig-trees. Both Paracrias species are diagnosed and illustrated. Males of P. ceratophaga sp. nov. are described. The association of Paracrias with weevil larvae is briefly discussed.

  9. Biology of two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae), recently invasive in the USA, reared on an ambrosia beetle artificial diet

    Science.gov (United States)

    1. Diet and rearing protocols were developed for two members of the cryptic Euwallacea fornicatus species complex, polyphagous shot hole borer (PSHB) and tea shot hole borer (TSHB) (Coleoptera: Curculionidae: Scolytinae), using sawdust from boxelder Acer negundo and avocado Persea americana. 2. Bio...

  10. Releases, distribution and abundance of Gratiana boliviana (Coleoptera:Chrysomelidae), a biological control agent of tropical soda apple (Solanum viarum, Solanaceae), in Florida

    Science.gov (United States)

    A biological control program against tropical soda apple (TSA) (Solanum viarum Dunal (Solanaceae)) released 176,643 Gratiana boliviana Spaeth (Coleoptera: Chrysomelidae) in Florida from 2003 to 2008. The spatial distribution of releases was clustered with more beetles released in south/central Flor...

  11. Desenvolvimento de Podisus nigrispinus (Dallas (Heteroptera, Pentatomidae com Zophobas confusa Gebien (Coleoptera, Tenebrionidae comparado à duas outras presas alternativas Development of Podisus nigrispinus (Dallas (Heteroptera, Pentatomidae fed with Zophobas confusa Gebien (Coleoptera, Tenebrionidae compared with two another alternative preys

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    Teresinha V Zanuncio

    1996-01-01

    Full Text Available Nymphs of Podisus nigrispinus (Dallas, 1851 were fed with Zophobas confusa Gebien, 1906, Tenebrio molitor Linnaeus, 1758 (Coleoptera, Tenebrionidae and Musca domestica Linnaeus, 1758 (Diptera, Muscidae under 25±0.5ºC, 60±10% of RH and photophase of 12h. Nymphal viability of P. nigrispinus were 64.0%, 80.0% and 92.0% with Z. confusa, M. domestica and T. molitor, respectively. No differences were found for pre-oviposition period, number of egg masses, number of eggs, egg viability, and longevity for females of this predator fed with any of these preys.

  12. Tipos de Curculionidae (Coleoptera: Curculionoidea) depositados en la colección entomológica del Museo de La Plata Types of Curculionidae (Coleoptera: Curculionoidea) housed at the Museo de La Plata entomological collection

    OpenAIRE

    Analía A. Lanteri; Suárez, Sonia M.; Guadalupe del Río, M.

    2003-01-01

    Esta contribución referida al material tipo de Curculionidae (Coleoptera: Curculionoidea) depositado en la colección entomológica del Museo de La Plata, actualiza la información sobre los tipos depositados durante los últimos diez años. Los 111 tipos examinados en este trabajo corresponden a 52 especies asignadas a las siguientes subfamilias: Curculioninae (5 spp.), Cryptorhynchinae (8 spp.), Cyclominae (4 spp.), Entiminae (31 spp.), y Erirhininae (4 spp.). Los autores de las especies son C. ...

  13. Influence of 60Co γ irradiation on fertility of Japanese pine sawyer beetle Monochamus alternatus hope (Coleoptera: Cerambycidae)

    International Nuclear Information System (INIS)

    The fertility of the Japanese pine sawyer beetle Monochamus alternatus (Coleoptera: Cerambycidae) irradiated with 60Co γ-rays was remarkable reduced at the doses of 30Gy, 35Gy and 40Gy, especially at 40Gy. When the non-irradiated females were coupled with the irradiated males first, and then coupled with non-irradiated males, the hatchability and the fertility had little higher but lower than the control. It explained that radiation has certain influence to the female gonad. It also has difference between the hatching rate and the amount of eggs in different match. (authors)

  14. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus.

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  15. Coleoptera Scarabaeoidea de la región de Teziutlán, Puebla, México

    OpenAIRE

    Adrián MUÑOZ-HERNÁNDEZ; Morón, Miguel Angel; Agustín ARAGÓN

    2008-01-01

    Con el propósito de evaluar la riqueza, abundancia y fenología de las especies de Coleoptera Scarabaeoidea o Lamellicornia de la región de Teziutlán, Puebla, se realizaron colectas mensuales entre mayo de 1999 y junio de 2000, en sitios con bosques de pino-encino-liquidámbar o zonas de cultivo situados entre 1,990 y 2,000 m de altitud. Se capturaron 2,082 ejemplares que representan a 59 especies de 29 géneros, 18 tribus y 7 subfamilias de Melolonthidae, Scarabaeidae y Passalidae, entre las qu...

  16. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    Abstract A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  17. Diversity and seasonality of Scarabaeinae (Coleoptera: Scarabaeidae in forest fragments in Santa Maria, Rio Grande do Sul, Brazil

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    PEDRO G. DA SILVA

    2013-05-01

    Full Text Available Scarabaeinae specimens were collected with the use of pitfall traps baited with human excrement, rotten meat and rotting banana, between May 2009 and April 2010, in three forest fragments in Santa Maria, Rio Grande do Sul, Brazil: ‘Morro do Elefante' (MOEL, ‘Morro do Cerrito' (MOCE and ‘Campus da Universidade Federal de Santa Maria' (UFSM. A total of 19,699 individuals belonging to 33 species were collected. Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. and Canthon lividus Blanchard, 1845, were the most abundant species. MOEL showed the greatest richness, MOCE the greatest abundance, while UFSM showed the lowest values of richness and abundance. The greatest similarity (qualitative and quantitative was found between MOEL and MOCE, while the lowest occurred between MOCE and UFSM. Only 51% of the species were common to all three fragments. The richness and abundance of Scarabaeinae were positively correlated with the air temperature. The richness of the fragment decreased according to the smaller size and the greater degree of disturbance of vegetation structure.Espécimes de Scarabaeinae foram coletados com o uso de armadilhas de queda iscadas com excremento humano, carne apodrecida e banana em decomposição, entre maio de 2009 e abril de 2010, em três fragmentos florestais em Santa Maria, Rio Grande do Sul, Brasil: Morro do Elefante (MOEL, Morro do Cerrito (MOCE e Campus da Universidade Federal de Santa Maria (UFSM. Um total de 19.699 indivíduos de 33 espécies foi coletado; Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. e Canthon lividus Blanchard, 1845 foram as espécies mais abundantes. MOEL apresentou a maior riqueza, MOCE a maior abundância, enquanto UFSM teve os menores valores de riqueza e abund

  18. Diversity and seasonality of Scarabaeinae (Coleoptera: Scarabaeidae in forest fragments in Santa Maria, Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    PEDRO G. DA SILVA

    2013-06-01

    Full Text Available Scarabaeinae specimens were collected with the use of pitfall traps baited with human excrement, rotten meat and rotting banana, between May 2009 and April 2010, in three forest fragments in Santa Maria, Rio Grande do Sul, Brazil: ‘Morro do Elefante’ (MOEL, ‘Morro do Cerrito’ (MOCE and ‘Campus da Universidade Federal de Santa Maria’ (UFSM. A total of 19,699 individuals belonging to 33 species were collected. Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. and Canthon lividus Blanchard, 1845, were the most abundant species. MOEL showed the greatest richness, MOCE the greatest abundance, while UFSM showed the lowest values of richness and abundance. The greatest similarity (qualitative and quantitative was found between MOEL and MOCE, while the lowest occurred between MOCE and UFSM. Only 51% of the species were common to all three fragments. The richness and abundance of Scarabaeinae were positively correlated with the air temperature. The richness of the fragment decreased according to the smaller size and the greater degree of disturbance of vegetation structure.Espécimes de Scarabaeinae foram coletados com o uso de armadilhas de queda iscadas com excremento humano, carne apodrecida e banana em decomposição, entre maio de 2009 e abril de 2010, em três fragmentos florestais em Santa Maria, Rio Grande do Sul, Brasil: Morro do Elefante (MOEL, Morro do Cerrito (MOCE e Campus da Universidade Federal de Santa Maria (UFSM. Um total de 19.699 indivíduos de 33 espécies foi coletado; Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. e Canthon lividus Blanchard, 1845 foram as espécies mais abundantes. MOEL apresentou a maior riqueza, MOCE a maior abundância, enquanto UFSM teve os menores valores de riqueza e

  19. Molecular identification of Epitrix potato flea beetles (Coleoptera: Chrysomelidae) in Europe and North America.

    Science.gov (United States)

    Germain, Jean-François; Chatot, Catherine; Meusnier, Isabelle; Artige, Emmanuelle; Rasplus, Jean-Yves; Cruaud, Astrid

    2013-06-01

    Epitrix species (Coleoptera: Chrysomelidae) feed mostly on plants from the family Solanaceae and some of them are major pests of potato crops. All Epitrix species are morphologically highly similar, which makes them difficult to identify and limits their study and management. Identification of species is mostly based on the observation of the genitalia and requires a high level of expertise. Here, we propose a tool to reliably identify all developmental stages of the most economically important Epitrix species feeding on potato in Europe and North America (Epitrix cucumeris, Epitrix similaris, Epitrix tuberis, Epitrix subcrinita and Epitrix hirtipennis). We first sequenced two DNA markers (mitochondrial cytochrome c oxidase I (COI) and nuclear internal transcribed spacer 2 (ITS2)) to test their effectiveness in differentiating among six Epitrix species (126 specimens). Morphospecies of Epitrix were well-differentiated by both DNA barcodes and no mitochondrial introgression was detected. Then, we developed an RFLP-based diagnostic method and showed that unambiguous species discrimination can be achieved by using the sole restriction enzyme TaqI on COI polymerase chain reaction products. The tool proposed here should improve our knowledge about Epitrix species biology, distribution and host range, three capacities that are particularly important in the detection and management of these pest species. Specifically, this tool should help prevent the introduction of E. tuberis and E. subcrinita in Europe and limit the spread of the recently introduced E. cucumeris and E. similaris, with minimal disruption to Solanaceae trade. PMID:23448201

  20. Sustainable management tactics for control of Phyllotreta cruciferae (Coleoptera: Chrysomelidae) on canola in Montana.

    Science.gov (United States)

    Reddy, Gadi V P; Tangtrakulwanich, Khanobporn; Miller, John H; Ophus, Victoria L; Prewett, Julie

    2014-04-01

    The crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae), has recently emerged as a serious pest of canola (Brassica napus L.) in Montana. The adult beetles feed on canola leaves, causing many small holes that stunt growth and reduce yield. In 2013, damage to canola seedlings was high (approximately 80%) in many parts of Montana, evidence that when flea beetles emerge in large numbers, they can quickly destroy a young canola crop. In the current study, the effectiveness of several biopesticides was evaluated and compared with two insecticides (deltamethrin and bifenthrin) commonly used as foliar sprays as well as seed treatment with an imidacloprid insecticide for the control of P. cruciferae under field conditions in 2013. The biopesticides used included an entomopathogenic nematode (Steinernema carpocapsae), two entomopathogenic fungi (Beauveria bassiana and Metarhizium brunneum), neem, and petroleum spray oils. The control agents were delivered in combination or alone in a single or repeated applications at different times. The plant-derived compound neem (azadirachtin), petroleum spray oil, and fatty acids (M-Pede) only showed moderate effect, although they significantly reduced leaf injuries caused by P. cruciferae and resulted in higher canola yield than the untreated control. Combined use of B. bassiana and M. brunneum in two repeated applications and bifenthrin in five applications were most effective in reducing feeding injuries and improving yield levels at both trial locations. This indicates that entomopathogenic fungi are effective against P. cruciferae, and may serve as alternatives to conventional insecticides or seed treatments in managing this pest. PMID:24772547

  1. Erotylidae (Insecta, Coleoptera of Poland – problematic taxa, updated keys and new records

    Directory of Open Access Journals (Sweden)

    Rafal Ruta

    2011-10-01

    Full Text Available New data concerning the occurrence of pleasing fungus beetles (Coleoptera: Erotylidae in Poland are given, with a focus on rare and difficult to identify Central European taxa. Cryptophilus cf. integer (Heer (Cryptophilinae is reported from the Polish territory for the first time based on adult and larval specimens collected in the Wielkopolska-Kujawy Lowland. Identification problems concerning species of Cryptophilus introduced to Europe are discussed. Triplax carpathica Reitter (Erotylinae is recorded from the Białowieża Primeval Forest, which is the first known non-Carpathian finding of this species, located in the close proximity of the Polish-Belarussian UNESCO World Heritage Site “Białowieża Forest”. Discussion of T. carpathica being conspecific with Siberian T. rufiventris Gebler is provided. New Polish localities of several other Erotylidae are reported, and an updated key to Central European species of Triplax is given. The Triplax key is supplemented with dorsal and ventral habitus images of all treated Triplax species. One of the rarest Central European erotyline species Combocerus glaber (Schaller is recorded from xerothermic grasslands in North-West Poland.

  2. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect

    International Nuclear Information System (INIS)

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5–7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1–4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. (author)

  3. Caracteres preimaginales y aspectos bionómicos de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae

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    Jerez, V.

    2011-12-01

    Full Text Available We describe the morphology and microstructure of the egg chorion, and the morphology of the first instar larva of Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini. Bionomical and distributional data on this species are also provided. To obtain eggs and larvae, couples were collected in the field and kept in rearing cages until oviposture and ecclosion. The structure and adornment of the egg exochorion, and the exterior morphological features of larvae were examined with electron scanning microscopy. The eggs of G. luczotii showed a rounded micropyle and a smooth exochorion, composed of hexagonal cells without aeropyles. The larvae of G. luczotii showed morphological characteristics suited for an edaphic life similar to that of Pedobionta: digging prothoracic legs, cephalic capsule with abundant sensilla, and well–developed pygopodium. We analysed the importance of larval morphology as an element for specific diagnosis and found that interspecific differences regarding frontal sensilla, clypeus shape, and anterior part of labrum, had a taxonomic value and possibly a phylogenetic value.

  4. Karyotype differentiation patterns in species of the subfamily Scarabaeinae (Scarabaeidae, Coleoptera).

    Science.gov (United States)

    Cabral-de-Mello, Diogo Cavalcanti; de Oliveira, Sárah Gomes; Ramos, Ituza Celeste; de Moura, Rita de Cássia

    2008-12-01

    The aim of this study was to describe the karyotype of species belonging to the subfamily Scarabaeinae (Coleoptera, Scarabaeidae) and to compile the conventional cytogenetic data available in the literature for this group. The karyotypes of ten species belonging to the tribes Canthonini, Coprini, Onthophagini and Phanaeini were analyzed by conventional staining. Eight of these species were described for the first time (Canthon aff carbonarius, Canthon chalybaeus, Coprophanaeus dardanus, Deltochilum aff amazonicum, Dichotomius geminatus, Oxysternon silenus, Phanaeus chalcomelas and Malagoniella aff astyanax) and two were redescribed (Diabroctis mimas and Digitonthophagus gazella) since their karyotypes differed from those previously published in the literature. Four species studied showed a diploid number of 2n=20 and a parachute type sex determining system and the karyotype was 2n=20,Xy in two species and 2n=18,Xy(p), 2n=19,X0, 2n=12,XY and 2n=14,neoXY in one each. The chromosome morphology of the different species varied, with the observation of metacentric, submetacentric, subacrocentric and acrocentric chromosomes. The X chromosome was predominantly meta or submetacentric in the species analyzed, whereas the y chromosome presented two arms or was punctiform. In conclusion, the subfamily Scarabaeinae comprises 120 species analyzed cytogenetically, and are observed the occurrence of five chromosome rearrangements (autosome-autosome and X-autosome fusions, pericentric inversions, fissions and loss of the y chromosome) that are related to the chromosome variability and evolution in the group. PMID:18495484

  5. Fruit Damage Patterns Caused by Ovipositing Females of Conotrachelus dimidiatus (Coleoptera: Curculionidae in Guava Trees

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    Felipe Tafoya

    2010-01-01

    Full Text Available We evaluated the damage patterns produced by females of the guava weevil Conotrachelus dimidiatus Champion, 1904 (Coleoptera: Curculionidae, according to the position of the damaged fruit in guava trees Psidium guajava L. in Calvillo, Aguascalientes, Mexico. The trees were subdivided in eight zones, and during one year the level of fruit lesions due to oviposition was registered. Results showed a higher level of damage in the upper and external zone of the trees (P≤.05. We found no significant differences in damage between the four cardinal points (P≥.05. During the year, the level of damage was recorded and was higher in the months of August and September (P≤.05 associated with rainfall (0.86, P=.06 and increase in temperature (0.84, P=.03. The most susceptible fruits were in the size range of 2.1–4.0 cm (polar diameter. The information from this study will be used to design and establish effective control strategies for the guava weevil, taking into account location of the most susceptible fruits, seasonality of the pest, and the abiotic factors.

  6. The Hydraulic Mechanism of the Unfolding of Hind Wings in Dorcus titanus platymelus (Order: Coleoptera

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    Jiyu Sun

    2014-04-01

    Full Text Available In most beetles, the hind wings are thin and fragile; when at rest, they are held over the back of the beetle. When the hind wing unfolds, it provides the necessary aerodynamic forces for flight. In this paper, we investigate the hydraulic mechanism of the unfolding process of the hind wings in Dorcus titanus platymelus (Oder: Coleoptera. The wing unfolding process of Dorcus titanus platymelus was examined using high speed camera sequences (400 frames/s, and the hydraulic pressure in the veins was measured with a biological pressure sensor and dynamic signal acquisition and analysis (DSA during the expansion process. We found that the total time for the release of hydraulic pressure during wing folding is longer than the time required for unfolding. The pressure is proportional to the length of the wings and the body mass of the beetle. A retinal camera was used to investigate the fluid direction. We found that the peak pressures correspond to two main cross-folding joint expansions in the hind wing. These observations strongly suggest that blood pressure facilitates the extension of hind wings during unfolding.

  7. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae

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    Andréa Roveré Franz

    2011-03-01

    Full Text Available Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae. Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf, Zingiber officinale (root and Mentha sp. (leaf. The efficacy of these oils was tested to control the rice weevil, S. oryzae, using hydrodistillation. Chemical analysis of the essential oils was carried out by gas chromatography. Major components of C. citratus were geranial (48% and neral (31%, of Z. officinale were α-zingibereno (13%, geranial (16%, neral (10% and α-farneseno (5% and of Mentha sp. was menthol (92%. Bioassays were carried out by fumigation and topical application. In topical application assays, the essential oil of C. citratus had greater toxicity (LC50 0.027 µL mL-1 and shorter exposure time than the oils of the other two plants. After 24 h and 48 h, 70% and 100% mortality of S. oryzae occurred, respectively. In fumigation assays, essential oil of Z. officinale had a lower LC50 (1.18 µL cm-2 and 70% mortality after 24 h exposure. Therefore, we recommend the use of essential oils of C. citratus and Z. officinale to control the rice weevil S. oryzae.

  8. On the spatial spread of the Rice Water Weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Erirhinidae, in Italy

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    Daniela Lupi

    2010-07-01

    Full Text Available A five year study has been made to establish the spread of the rice water weevil Lissorhoptrus oryzophilus (Coleoptera: Erirhinidae in Northern Italy. Data obtained with GPS from 2005 throughout 2009 were first georeferenced with SW ArcGis® 9.2, then overlapped and compared to the map of the European environmental landscape based on the interpretation of satellite images (CORINE Land Cover map and to the hydrographic chart CT10 (Technical Regional map 10000. The analysis of the radial rate of spread per year indicates a deceleration in the expansion from 10.864 ± 6.801 km/year in 2005 to 5.318 ± 1.401 km/year in 2009. In five years the weevil has expanded its distribution in nearly all rice paddies in Lombardy and Piedmont, over an area of about 200,000 ha, which correspond to 86% of the total Italian rice area. Its expansion is thought to follow a type of stratified dispersal, due both to insect adult active dispersal and to accidental movements caused by human transportation.

  9. Naturally-Occurring Entomopathogenic Fungi on Three Bark Beetle Species (Coleoptera: Curculionidae in Bulgaria

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    Slavimira A. Draganova

    2010-01-01

    Full Text Available Bark beetles (Coleoptera: Curculionidae: Scolytinae belong to one of the most damaging groups of forest insects and the activity of their natural enemies –pathogens, parasitoids,parasites or predators suppressing their population density,is of great importance. Biodiversity of entomopathogenic fungi on bark beetles in Bulgaria has been investigated sporadically. The aim of this preliminary study was to find, identify and study morphological characteristics of fungal entomopathogens naturally-occurring in populations of three curculionid species – Ips sexdentatus Boern, Ips typographus (L. and Dryocoetes autographus (Ratz.. Dead pest adults were found under the bark of Pinus sylvestris and Picea abies trees collectedfrom forests in the Maleshevska and Vitosha Mountains. Fungal pathogens were isolated into pure cultures on SDAY (Sabouraud dextrose agar with yeast extract and were identified based on morphological characteristics both on the host and in a culture.Morphological characteristics of the isolates were studied by phenotypic methods. The fungal isolates obtained from dead adults of Ips sexdentatus, Ips typographus and D. autographus were found to belong to the species Beauveria bassiana (Bals. – Criv. Vuillemin,Beauveria brongniartii (Saccardo Petch and Isaria farinosa (Holmsk. Fries (anamorph Ascomycota, Sordariomycetes: Hypocreales, Cordycipitaceae. Morphological traits of the isolates are described.

  10. Pimenta pseudocaryophyllus Derivatives: Extraction Methods and Bioactivity Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae).

    Science.gov (United States)

    Ribeiro, L P; Ansante, T F; Niculau, E S; Pavarini, R; Silva, M F G F; Seffrin, R C; Vendramim, J D

    2015-12-01

    Plant-based insecticides can play an important role in integrated insect pest management (IPM), especially in protecting stored grains. The aim of this study was to evaluate the bioactivity of derivatives (powder, ethanolic extract, and essential oil (EO)) from the leaves of Pimenta pseudocaryophyllus (Myrtaceae), a Brazilian native species, against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), the main insect pest of stored corn. The powder and essential oil prepared from leaves showed a repellent effect. Moreover, the EO exhibited promising insecticidal activity through residual contact (LC50 = 1522 mg kg(-1)) and significantly decreased the F 1 progeny and the percentage of damaged grains. However, the essential oil obtained from P. pseudocaryophyllus leaves did not result in significant mortality of S. zeamais adults after 72 h of exposure by fumigation in concentrations up to 400 μL L(-1) of air. Based on GC-MS analysis, 20 compounds were identified in the essential oil of P. pseudocaryophyllus leaves, being chavibetol (38.14%), methyl eugenol (11.35%), and terpinolene (9.17%) as the major constituents. Essential oil from P. pseudocaryophyllus leaves is an interesting source of compounds with grain-protectant properties and should be analyzed in future studies aiming to develop new bioinsecticides to use in the IPM of stored grains. PMID:26285912

  11. Coexistence and Competition between Tomicus yunnanensis and T. minor (Coleoptera: Scolytinae in Yunnan Pine

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    Rong Chun Lu

    2012-01-01

    Full Text Available Competition and cooperation between bark beetles, Tomicus yunnanensis Kirkendall and Faccoli and Tomicus minor (Hartig (Coleoptera: Scolytinae were examined when they coexisted together in living Yunnan pine trees (Pinus yunnanensis Franchet in Yunnan province in Southwest China. T. yunnanensis bark beetles were observed to initiate dispersal from pine shoots to trunks in November, while the majority of T. minor begins to transfer in December. T. yunnanensis mainly attacks the top and middle parts of the trunk, whereas T. minor mainly resides in the lower and middle parts of the trunk. The patterns of attack densities of these two species were similar, but with T. yunnanensis colonizing the upper section of the trunk and T. minor the lower trunk. The highest attack density of T. Yunnanensis was 297 egg galleries/m2, and the highest attack density of T. minor was 305 egg galleries/m2. Although there was significant overlap for the same bark areas, the two species generally colonize different areas of the tree, which reduces the intensity of competition for the relatively thin layer of phloem-cambium tissues where the beetles feed and reside.

  12. Factors Affecting Pheromone Production by the Pepper Weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae and Collection Efficiency

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    Fred J. Eller

    2014-11-01

    Full Text Available Several factors affecting pheromone production by male pepper weevils, Anthonomus eugenii Cano (Coleoptera: Curculionidae as well as collection efficiency were investigated. Factors studied included: porous polymer adsorbents (Tenax versus Super Q, male age, time of day, male density, and male diet. Super Q was found to be a superior adsorbent for the male-produced alcohols and geranic acid as well as the plant-produced E-β-ocimene. Pheromone production increased with male age up to about age 15 days old and then tapered off. Male pepper weevils produced the highest amount of pheromone between noon and 2 pm (i.e., 4 to 6 h after “lights on” and were producing ca. 800 ng/h during this period. Thereafter, pheromone production decreased and was extremely low during the scotophase (i.e., ca. 12 ng/h. Male pepper weevil density had a significant effect on both release rate and pheromone composition. Pheromone production on a per male basis was highest for individual males and the percentage of geranic acid in the blend was lowest for individual males. Male pepper weevils produced only extremely low amounts of pheromone when feeding on artificial diet; however, they produced very high amounts when on fresh peppers. Together, this information will be useful in designing better attractant lures for pepper weevils.

  13. The complete mitochondrial genome of Epicauta chinensis (Coleoptera: Meloidae) and phylogenetic analysis among Coleopteran insects.

    Science.gov (United States)

    Du, Chao; He, Shilin; Song, Xuhao; Liao, Qi; Zhang, Xiuyue; Yue, Bisong

    2016-03-10

    The blister beetle is an important resource insect due to its defensive substance cantharidin, which was widely used in pharmacology and plant protection. We determined the complete mitochondrial genome of Epicauta chinensis Laporte (Coleoptera: Tenebrionoidae: Meloidae). The circular genome is 15,717 bp long, encoding 13 protein-coding genes (PCGs), two ribosomal RNAs and 22 tRNAs and containing a A+T-rich region with gene arrangement identical to other Coleopteran species. Twelve PCGs start with typical ATN codon, while ATP8 gene initiate with GTT for first report in Insecta. All PCGs terminate with conventional stop codon TAA or TAG. All tRNAs in E. chinensis are predicted to fold into typical cloverleaf secondary structure, except tRNA-Ser(AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. The secondary structure of lrRNA and srRNA comprises 48 helices and 32 helices respectively. The 1101 bp A+T-rich region contains a 15 bp poly-T stretch and microsatellite-like repeats rather than large tandem repetitive sequences. Phylogenetic analysis, based on 13 PCGs of 45 Coleopteran species, show that E. chinensis grouped with Tenebrionidae species. It also support the topology of (((Chrysomelidae+Curculionoidea)+(Cucujoidea+Cleroidea))+Tenebrionoidea) within Cucujiformia. PMID:26707213

  14. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae

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    M.M. Kiradoo

    2011-03-01

    Full Text Available The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insecticides, there is an utmost need to search for some alternate methods for protection against the damage caused by insects. The leaves of the select plants viz., Ocimum basilicum, O. sanctum and Mentha spicata were employed in the form of various formulations and mortality of the pest insect was assessed. A significant increase in mortality of C. chinensis was observed in all the experimental sets as compared to normal and control sets during the present study. When comparisons were made on the basis of ANOVA to compare the effect of the three plants on mortality, it was found that the treatments of O. basilicum were superior over M. spicata and O. sanctum. The findings overall suggest that all the three plants screened have a potential to be used against the pest C. chinensis and can be employed as an alternate to chemical insecticides in household and storehouses to minimize the infestation and damage caused by the bruchid.

  15. Preliminary Diversity Of Dung Beetles (Coleoptera: Scarabaeinae Attracting By Human Dung (Calvario, Meta

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    Ruth Mariela Castillo Morales

    2011-12-01

    Full Text Available In the Private Reserve, Refugio del Oso de Anteojos (El Calvario-Meta, the diversity of dung beetles was studied (Coleoptera: Scarabaeinae, grouped around different types of vegetation (Montane Rain Forest, Rainforest Premontane, Lower Montane Rain Forest, by using pitfall traps baited with human dung. During the samplings carried out in the rainy station on 2008, were captured a total of 177 individuals belonging to 9 species. The tribe Canthonini was the most representative with 87.5%, of the described species, followed by Aechini 10.8%, and Onthophagini 1.7%. The dominant species was Canthon lituratus in the three vegetation units: Lower montane rain forest was the richest (9 species and abundance (86 individuals, compared with other environments sampled. By calculating the complementarity index, shows a similar species composition in the three habitats sampled, possibly, related to the floristic composition presented in each one of them.

  16. Characterization of the complete mitochondrial genome of the firefly, Luciola substriata (Coleoptera: Lampyridae).

    Science.gov (United States)

    Mu, Feng-Juan; Ao, Liang; Zhao, Hua-Bin; Wang, Kai

    2016-09-01

    The firefly, Luciola substriata (Coleoptera: Lampyridae), is an aquatic firefly species, whose larvae inhabit ponds or lakes. Here we present the complete mitochondrial (mt) genome of the firefly (GenBank accession number KP313820) and provide its annotation. This circular genome is 16,248 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a non-coding AT-rich region. Similar to other firefly species, the base composition of this mitochondrial genome is also biased toward A and T (44.09% A, 34.00% T, 12.89% C, and 9.01% G). All 13 protein-coding genes start with a typical mitochondrial start codon, and terminate with a usual stop codon TAA, or TAG or a single T. The non-coding AT-rich region (1636 bp in length) include one (A)20, and two (T)15 tandem repeats, and one (AAT)5 element. This mitochondrial genome sequence will promote a better understanding for firefly evolution in the future. PMID:25714154

  17. Observations on the Cave-Associated Beetles (Coleoptera of Nova Scotia, Canada

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    Moseley M.

    2009-07-01

    Full Text Available The cave-associated invertebrates of Nova Scotia constitute a fauna at a very early stage of post-glacial recolonization. TheColeoptera are characterized by low species diversity. A staphylinid Quedius spelaeus spelaeus, a predator, is the only regularlyencountered beetle. Ten other terrestrial species registered from cave environments in the province are collected infrequently. Theyinclude three other rove-beetles: Brathinus nitidus, Gennadota canadensis and Atheta annexa. The latter two together with Catopsgratiosus (Leiodidae constitute a small group of cave-associated beetles found in decompositional situations. Quedius s. spelaeusand a small suite of other guanophiles live in accumulations of porcupine dung: Agolinus leopardus (Scarabaeidae, Corticariaserrata (Latrididae, and Acrotrichis castanea (Ptilidae. Two adventive weevils Otiorhynchus ligneus and Barypeithes pellucidus(Curculionidae collected in shallow cave passages are seasonal transients; Dermestes lardarius (Dermestidae, recorded fromone cave, was probably an accidental (stray. Five of the terrestrial beetles are adventive Palaearctic species. Aquatic beetles arecollected infrequently. Four taxa have been recorded: Agabus larsoni (Dytiscidae may be habitual in regional caves; another Agabussp. (probably semivittatus, Dytiscus sp. (Dytiscidae, and Crenitis digesta (Hydrophilidae are accidentals. The distribution andecology of recorded species are discussed, and attention is drawn to the association of beetles found in a Nova Scotia “ice cave”.

  18. Population dynamics and spatial distribution of Abaris basistriata Chaudoir, 1873 (Coleoptera: Carabidae

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    Ivan Carlos Fernandes Martins

    2016-02-01

    Full Text Available ABSTRACT Abaris basistriata, a beetle species dominant in agroecosystems and natural habitats, may benefit from the establishment of nearby refuge areas or crop field centers. To confirm this hypothesis, we analyzed the spatial distribution of the species and verified the population dynamics of this predator in a soybean/corn rotation crop and a central refuge area. The 1-ha experimental area was divided in half by a range of herbaceous plants (2 m in width and 80 m in length. Beetle samples were collected using pitfall traps every fortnight during the in-season and every month during the off-season (a total of 27 sampling occurrences. Population fluctuation was analyzed by correlating the total number of specimens with plant phenology. We used multiple regression analysis with variable (stepwise selection to examine the influence of meteorological factors on species occurrence. To determine the spatial distribution, data were analyzed using dispersion indices and probabilistic models based on the Coleoptera frequency distribution. Distribution visualization was assessed using a linear interpolation map. A total of 143 A. basistriata specimens were collected, with 83 from the soybean/corn area and 60 from the refuge area. Periods of large population size occurred during a season with high rainfall and high maximum and minimum temperatures. On the basis of the spatial distribution analysis of A. basistriata, it is likely that the beetles occur in an aggregate form, preferably in the refuge area.

  19. Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil

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    Andreia Mauruto Chernaki-Leffer

    2011-03-01

    Full Text Available Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer, is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvos. Larvae were fed rabbit feed wetted with a triflumuron-water solution. Concentration-mortality regressions were estimated using Probit analysis and resistance ratios were calculated based on the susceptible population. Among the field populations evaluated, cypermethrin LC50 values for adults, ranged from 68.1 to 6,263 ng (AI/cm². LC50 values for adults challenged with dichlorvos ranged from 10.3 to 1,385 ng (AI/cm². One population from Pato Branco showed reduced susceptibility to triflumuron (LC50 = 272 µg (AI/ml of solution when compared to the most susceptible population (LC50 = 109.8 µg (AI/ml. Application of cypermethrin and dichlorvos analogues should be managed with caution to minimize insecticide resistance problems.

  20. Pitfall Traps and Mini-Winkler Extractor as Complementary Methods to Sample Soil Coleoptera.

    Science.gov (United States)

    Carneiro, A C; Batistella, D A; Battirola, L D; Marques, M I

    2016-02-01

    We compared abundance, species richness, and capture efficiency with pitfall traps and mini-Winkler extractors to examine their use as complementary methods for sampling soil Coleoptera during dry (2010) and high water seasons (2011) in three areas, including inundated and non-inundated regions, in the Pantanal of Poconé, Mato Grosso, Brazil. We paired treatments with two 10 × 10 m plots in inundated and non-inundated locations that were repeated three times in each location for a total of 18 plots. In each plot, we used nine pitfall traps and collected 2 m(2) of leaf litter and surface soil samples with mini-Winkler extractors. We collected a total of 4260 adult beetles comprising 36 families, 113 genera, and 505 species. Most were caught in pitfalls (69%) and the remainder in the mini-Winkler extractors (31%). Each method provided distinct information about the beetle community: 252 species were captured only in pitfall traps, 147 using only the mini-Winkler extractors, and these methods shared another 106 species. Pitfall and mini-Winkler contribute in different ways for the sampling of the soil beetle community, and so they should be considered complementary for a more thorough assessment of community diversity. PMID:26493175

  1. Oil pipeline corridor through an intact forest alters ground beetle (Coleoptera: Carabidae) assemblages in southeastern Ohio.

    Science.gov (United States)

    Silverman, Bareena; Horn, David J; Purrington, Foster F; Gandhi, Kamal J K

    2008-06-01

    Litter-dwelling ground beetle (Coleoptera: Carabidae) assemblages were monitored 1 yr after the construction of a corridor for installation of an oil pipeline along a xeric ridge-top forest in southeastern Ohio. After the creation of the corridor, three distinct habitats were evident in these sites: open corridor, ecotone areas around the corridor, and undisturbed forest interior. Carabidae were collected using directional pitfall traps that were placed parallel and perpendicular to the corridor in each of the three habitats. Results indicate that more carabids were present in the ecotone than in the other two habitats. Carabid diversity as estimated by rarefaction was highest in the corridor followed by ecotone and forest interior. Generalist and forest specialists such as Synuchus impunctatus (Say), Carabus goryi Dejean, and Pterostichus trinarius (Casey) were present in greater numbers in the forest interior and ecotone assemblages. In contrast, open-habitat specialists such as Harpalus pensylvanicus (DeGeer) and Selenophorus opalinus (LeConte) were present in greater numbers in the corridor assemblages. Carabid assemblages of the corridor were distinct from those of the ecotone and forest interior, whereas the latter two habitats had very similar assemblages. The successional pathway of the corridor carabid assemblage will therefore be likely different from that of the forest interior and ecotone. Overall, results indicate that construction of the oil pipeline corridor had significant short-term effects on the carabid numbers, diversity, and species composition because of ensuing habitat changes and fragmentation of the forest. PMID:18559178

  2. Stenusine, an antimicrobial agent in the rove beetle genus Stenus (Coleoptera, Staphylinidae)

    Science.gov (United States)

    Lusebrink, Inka; Dettner, Konrad; Seifert, Karlheinz

    2008-08-01

    Stenusine is well known as the alkaloid, discharged by the rove beetle, genus Stenus Latreille (Coleoptera, Staphylinidae). The Stenus beetles employ the alkaloid as an escape mechanism when on water surfaces. In the case of danger, they lower their abdomen and emit stenusine from their pygidial glands. Stenusine shows a low surface tension and therefore a high spreading pressure; these properties propel the beetle quickly over the water. Many Steninae do not live in habitats with open waters, but in detritus, leaf litter, mosses, etc. This raises the possibility that stenusine might also have another function, e.g., as antibiotic or fungicide. Stenus beetles show an intense grooming behaviour. With gas chromatography mass spectrometry analyses we could prove that they cover themselves with their secretion. To tests its antimicrobial properties we conducted agar diffusion tests with stenusine and norstenusine, another substance that is abundant in most Stenus species. Both compounds have an antimicrobial effect on entomopathogenic bacteria and fungi. Stenusine not only allows for an extraordinary method of locomotion on water surfaces, it also protects the Steninae from being infested with microorganisms.

  3. Demographic comparison and population projection of Rhynchophorus ferrugineus (Coleoptera: Curculionidae) reared on sugarcane at different temperatures.

    Science.gov (United States)

    Peng, Lu; Miao, Yunxin; Hou, Youming

    2016-01-01

    Understanding how temperature affects fitness is important for conservation and pest management, especially in the era of global climate change. Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae) is a worldwide pest of many economically important crops. Although much is known about this pest's life cycle, its adaptability to different temperatures is not fully understood. Here, we used age- and stage-specific life tables to investigate the effects of temperature on fitness-related traits and demographic parameters of R. ferrugineus under eight constant temperature regimens in the laboratory. The growth potential of these populations was also evaluated. The greatest longevity for males and females was 158.0 d at 24 °C and 144.5 d at 21 °C, respectively, but mean total fecundity was the highest at 27 °C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0) increased initially at low temperatures and then decreased. All metrics reached a maximum at 27 °C and a minimum at 36 °C. Mean generation times (T ) decreased across the temperature range with a minimum at 36 °C. Our results indicate that the optimum temperature for growth of R. ferrugineus was approximately 27 °C. Our work will be of value for developing strategies for control management of this pest species. PMID:27545594

  4. Notas e novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae

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    Ubirajara R. Martins

    2007-01-01

    Full Text Available Novas espécies descritas do Brasil: Hypsioma carioca sp. nov. (Rio de Janeiro e Hesychotypa maraba sp. nov. (Pará; do Equador: Sternycha ecuatoriana sp. nov. (Pichincha; da Bolívia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. e Alexera secunda sp. nov. Transfere-se Hesycha strandi (Breuning, 1943 para o gênero Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 é considerada sinônima de H. miniata Thomson, 1868. Novo registro (Trinidad e figura são dados para Trachysomus surdus.Notes and new species of Onciderini (Coleoptera, Cerambycidae, Lamiinae. New species described from Brazil: Hypsioma carioca sp. nov. (Rio de Janeiro and Hesychotypa maraba sp. nov. (Pará; from Ecuador: Sternycha ecuatoriana sp. nov. (Pichincha; from Bolivia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. and Alexera secunda sp. nov. Hesycha strandi (Breuning, 1943 is transferred to the genus Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 is considered a synonym of H. miniata Thomson, 1868. New record (Trinidad and figure for Trachysomus surdus Dillon & Dillon, 1946 are given.

  5. Stenusine, an antimicrobial agent in the rove beetle genus Stenus (Coleoptera, Staphylinidae).

    Science.gov (United States)

    Lusebrink, Inka; Dettner, Konrad; Seifert, Karlheinz

    2008-08-01

    Stenusine is well known as the alkaloid, discharged by the rove beetle, genus Stenus Latreille (Coleoptera, Staphylinidae). The Stenus beetles employ the alkaloid as an escape mechanism when on water surfaces. In the case of danger, they lower their abdomen and emit stenusine from their pygidial glands. Stenusine shows a low surface tension and therefore a high spreading pressure; these properties propel the beetle quickly over the water. Many Steninae do not live in habitats with open waters, but in detritus, leaf litter, mosses, etc. This raises the possibility that stenusine might also have another function, e.g., as antibiotic or fungicide. Stenus beetles show an intense grooming behaviour. With gas chromatography-mass spectrometry analyses we could prove that they cover themselves with their secretion. To tests its antimicrobial properties we conducted agar diffusion tests with stenusine and norstenusine, another substance that is abundant in most Stenus species. Both compounds have an antimicrobial effect on entomopathogenic bacteria and fungi. Stenusine not only allows for an extraordinary method of locomotion on water surfaces, it also protects the Steninae from being infested with microorganisms. PMID:18392795

  6. A multilayer micromechanical model of the cuticle of Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae).

    Science.gov (United States)

    Andrew Jansen, M; Singh, Sudhanshu S; Chawla, Nikhilesh; Franz, Nico M

    2016-08-01

    Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae), is a weevil species common throughout the southwestern United States that uses its rostrum - a very slender, curved, beak-like projection of the head - to excavate tunnels in plant organs (such as acorns) for egg laying (oviposition). Once the apical portion of the rostrum has been inserted into the preferred substrate for oviposition, the female begins rotating around the perimeter of the hole, elevating her head by extending the fore-legs, and rotating the head in place in a drilling motion. This action causes significant elastic deformation of the rostrum, which will bend until it becomes completely straight. To better understand the mechanical behavior of the cuticle as it undergoes deformation during the preparation of oviposition sites, we develop a comprehensive micro/macro model of the micromechanical structure and properties of the cuticle, spanning across all cuticular regions, and reliably mirroring the resultant macroscale properties of the cuticle. Our modeling approach relies on the use of multi-scale, hierarchical biomaterial representation, and employs various micromechanical schemata - e.g., Mori-Tanaka, effective field, and Maxwell - to calculate the homogenized properties of representative volume elements at each level in the hierarchy. We describe the configuration and behavior of this model in detail, and discuss the theoretical implications and limitations of this approach with emphasis on future biomechanical and comparative evolutionary research. Our detailed account of this approach can thereby serve as a methodological template for exploring the biomechanical behavior of new insect structures. PMID:27189867

  7. Transcriptomic Analysis of Eucryptorrhynchus chinensis (Coleoptera: Curculionidae) Using 454 Pyrosequencing Technology

    Science.gov (United States)

    Liu, Zhen-Kai; Wen, Jun-Bao

    2016-01-01

    Eucryptorrhynchus chinensis Olivier (Coleoptera: Curculionidae) is one of the most important pests of Ailanthus altissima; however, so far, no studies on the genome or transcriptome of E. chinensis have been reported. Using the Roche 454 FLX Titanium platform, an RNA pool obtained from E. chinensis eggs, larva, pupae, and adults was sequenced and assembled de novo to achieve maximum diversity of sampled transcripts. We obtained 1,441,137 (∼518 Mb) raw reads with an average length of 360 bp. After trimming, 89% qualified reads were produced and assembled into 35,509 isotigs with an average length of 440 bp, N50 of 1,048 bp, and 111,643 singletons. We generated 87,894 unigenes following a cluster analysis of the isotigs and singletons, and then functionally annotated the unigenes with gene descriptions. We obtained 23,363 GO assignments, and 12,724 unigenes were assigned to KOG. Based on these annotations, 294 biochemical pathways involved in growth, reproduction, and stress or immune responses were predicted. A total of 659,026 single nucleotide variants and 6,112 simple sequence repeats were detected. Our data provide comprehensive information on the sequence and possible functions of E. chinensis transcripts.

  8. Endemic epigean Tenebrionids (Coleoptera: Tenebrionidae) from the Andean Region: exploring the patagonian-diversification hypothesis.

    Science.gov (United States)

    Carrara, Rodolfo; Flores, Gustavo E

    2015-01-01

    Tenebrionidae is a diverse insect family of Coleoptera that shows high levels of endemicity in epigean species. For the Andean region, which is divided into three subregions: Central Chilean, Subantarctic and Patagonian, it has been hypothesized that epigean tenebrionids have diversified in the Patagonian subregion and subsequently, they dispersed to Subantarctic and Central Chilean subregions. In this work, based on information obtained from museum collections and scientific studies, we presented the first list of endemic epigean tenebrionids from the Andean region with their taxonomic arrangement and geographic distribution. Moreover, we used these data to explore the veracity of the Patagonian-diversification hypothesis. A total of 416 species grouped into six subfamilies, 17 tribes and 41 genera were identified as endemic to the Andean region. Considering the spatial distribution it was observed that subfamilies, tribes, genera and species were unequally distributed across subregions. Results did not support the Patagonian-diversification hypothesis; to the contrary, they were more concordant with processes of isolation among subregions that have promoted speciation by interrupting gene flow among populations, resulting in endemism because species can not expand their range sizes. Finally, we discuss the implications of our findings to be considered in biodiversity conservation, because endemic species, by their high extinction risk, are primary targets in conservation strategies. PMID:26623788

  9. Pathology of Yersinia entomophaga MH96 towards Costelytra zealandica (Coleoptera; Scarabaeidae) larvae.

    Science.gov (United States)

    Hurst, Mark Robin Holmes; van Koten, Chikako; Jackson, Trevor Anthony

    2014-01-01

    The bacterium Yersinia entomophaga was isolated from larvae of the New Zealand grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), found in soil. Following ingestion of a lethal dose of bacteria, larvae of C. zealandica reduced feeding activity and movement. After approximately 4h infected larvae convulsed and regurgitated dark digestive fluid and expelled frass pellets leaving the midgut empty and the larva amber in appearance. In the initial stages of infection, ingested bacteria were mostly contained within the peritrophic membrane and expelled with the gut fluid or transferred into the hind gut. While few Y. entomophaga were associated with the midgut epithelial cells, by 24h cells were swelling and bursting with vesicles being expelled into the midgut lumen. By 48h, bacteria had entered the haemocoel and the midgut cells had further deteriorated. After 72h, the cellular remnants were totally detached from the basal membrane the infected insects were filled with bacteria and moribund or dead with septicaemia. Mortality was directly proportional to dose and time after infection. By applying a range of doses, the LD50 was determined as 2.9×10(4)Y. entomophaga per C. zealandica larva, with an LT50 of 2.94days for doses of>1×10(5) per larva. Ingestion of low doses of bacteria did not inhibit feeding activity but led more slowly to death. By time of death, Y. entomophaga had multiplied, approximately 500 fold, in the cadavers of the infected larvae. PMID:24291403

  10. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

    Science.gov (United States)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2015-06-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. PMID:26470205

  11. Ambrosia Beetle (Coleoptera: Scolytidae) Species, Flight, and Attack on Living Eastern Cottonwood Trees.

    Energy Technology Data Exchange (ETDEWEB)

    Coyle, D R; D.C. Booth: M.S. Wallace

    2005-12-01

    ABSTRACT In spring 2002, ambrosia beetles (Coleoptera: Scolytidae) infested an intensively managed 22-ha tree plantation on the upper coastal plain of South Carolina. Nearly 3,500 scolytids representing 28 species were captured in ethanol-baited traps from 18 June 2002 to 18 April 2004. More than 88% of total captures were exotic species. Five species [Dryoxylon onoharaensum (Murayama), Euwallacea validus (Eichhoff), Pseudopityophthorus minutissimus (Zimmermann), Xyleborus atratus Eichhoff, and Xyleborus impressus Eichhoff]) were collected in South Carolina for the first time. Of four tree species in the plantation, eastern cottonwood, Populus deltoides Bartram, was the only one attacked, with nearly 40% of the trees sustaining ambrosia beetle damage. Clone ST66 sustained more damage than clone S7C15. ST66 trees receiving fertilization were attacked more frequently than trees receiving irrigation, irrigation_fertilization, or controls, although the number of S7C15 trees attacked did not differ among treatments. The study location is near major shipping ports; our results demonstrate the necessity for intensive monitoring programs to determine the arrival, spread, ecology, and impact of exotic scolytids.

  12. Evolution of subterranean diving beetles (Coleoptera: Dytiscidae: Hydroporini, Bidessini) in the arid zone of Australia.

    Science.gov (United States)

    Leys, Remko; Watts, Chris H S; Cooper, Steve J B; Humphreys, William F

    2003-12-01

    Calcrete aquifers in arid inland Australia have recently been found to contain the world's most diverse assemblage of subterranean diving beetles (Coleoptera: Dytiscidae). In this study we test whether the adaptive shift hypothesis (ASH) or the climatic relict hypothesis (CRH) is the most likely mode of evolution for the Australian subterranean diving beetles by using a phylogeny based on two sequenced fragments of mitochondrial genes (CO1 and 16S-tRNA-ND1) and linearized using a relaxed molecular clock method. Most individual calcrete aquifers contain an assemblage of diving beetle species of distantly related lineages and/or a single pair of sister species that significantly differ in size and morphology. Evolutionary transitions from surface to subterranean life took place in a relatively small time frame between nine and four million years ago. Most of the variation in divergence times of the sympatric sister species is explained by the variation in latitude of the localities, which correlates with the onset of aridity from the north to the south and with an aridity maximum in the Early Pliocene (five mya). We conclude that individual calcrete aquifers were colonized by several distantly related diving beetle lineages. Several lines of evidence from molecular clock analyses support the CRH, indicating that all evolutionary transitions took place during the Late Miocene and Early Pliocene as a result of aridification. PMID:14761060

  13. Culturable bacterial microbiota of Plagiodera versicolora (L.) (Coleoptera: Chrysomelidae) and virulence of the isolated strains.

    Science.gov (United States)

    Demirci, Meryem; Sevim, Elif; Demir, İsmail; Sevim, Ali

    2013-05-01

    Plagiodera versicolora (Laicharting, 1781) (Coleoptera: Chrysomelidae) is an important forest pest which damages many trees such as willow, poplar, and hazelnut. In order to find new microbes that can be utilized as a possible microbial control agent against this pest, we investigated the culturable bacterial flora of it and tested the isolated bacteria against P. versicolora larvae and adults. We were able to isolate nine bacteria from larvae and adults. The isolates were characterized using a combination of morphological, biochemical, and physiological methods. Additionally, we sequenced the partial sequence of the 16S rRNA gene to verify conventional identification results. Based on characterization studies, the isolates were identified as Staphylococcus sp. Pv1, Rahnella sp. Pv2, Rahnella sp. Pv3, Rahnella sp. Pv4, Rahnella sp. Pv5, Pantoea agglomerans Pv6, Staphylococcus sp. Pv7, Micrococcus luteus Pv8, and Rahnella sp. Pv9. The highest insecticidal activity against larvae and adults was obtained from M. luteus Pv8 with 50 and 40 % mortalities within 10 days after treatment, respectively. Extracellular enzyme activity of the bacterial isolates such as amylase, proteinase, lipase, cellulose, and chitinase was also determined. Consequently, our results show that M. luteus Pv8 might be a good candidate as a possible microbial control agent against P. versicolora and were discussed with respect to biocontrol potential of the bacterial isolates. PMID:23054688

  14. Morfología del tracto digestivo y sistema reproductor femenino de Compsus sp. (Coleoptera: Curculionidae Morphology of the digestive tract and female reproductive system of Compsus sp. (Coleoptera: Curculionidae

    Directory of Open Access Journals (Sweden)

    JOSÉ DAVID RUBIO G

    2007-12-01

    Full Text Available Se realizó el estudio morfológico del tracto digestivo y sistema reproductor femenino del picudo de los cítricos, Compsus sp. (Coleoptera: Curculionidae. El tracto digestivo presentó un arreglo típico descrito para otros insectos. Se encontraron diferencias morfológicas, sin embargo, en el buche en donde se observan unas estructuras espinosas. A nivel del mesenterón, se observó que el ventrículo anterior se encuentra dividido en sacos de tamaño medio y en el ventrículo posterior se proyectan numerosos ciegos gástricos. El sistema reproductor de la hembra mostró un arreglo estructural común a especímenes de la familia Curculionidae.A morphological examination was conducted on the digestive tract and the female reproductive system of the citrus weevil, Compsus sp. (Coleoptera: Curculionidae. The digestive tract of Compsus sp. showed a typical arrangement as described for other insects. Morphological differences, however, were found in the crop of the foregut, which exhibited several spine-like structures. At the midgut level, the anterior ventricle was divided into medium-size sacs and numerous gastric caecae projected into the posterior ventricle. The female reproductive system showed a structural arrangement common to individuals of the family Curculionidae.

  15. Cowpea Vicilins: Fractionation of Urea Denatured Sub-Units and Effects on Callosobruchus maculatus F. (Coleoptera: Bruchidae Development

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    Antônio Chagas Mota

    2002-03-01

    Full Text Available Vicilins (7S storage globulins isolated from cowpea (Vigna unguiculata L. seeds which were susceptible (S and resistant (R to the cowpea weevil (Callosobruchus maculatus F., Coleoptera: Bruchidae were denatured by urea and fractionated by ion-exchange chromatography. Isolated fractions were incorporated in artificial seeds for assessment of their toxicity to C. maculatus. The most acidic fractions of both susceptible (CE-31 cultivar and resistant (IT81D-1045 line seeds were shown to affect development and survival of the bruchid. Results indicated that vicilin polypeptides of toxic nature were expressed in both types of storage globulins although at different levels.Vicilinas (globulinas de reserva 7S isoladas de sementes de feijão-de-corda (Vigna unguiculata L., susceptíveis (S e resistentes (R ao caruncho/gorgulho (Callosobruchus maculatus F., Coleoptera: Bruchidae foram desnaturadas por uréia e fracionadas por cromatografia de troca iônica. As frações isoladas foram incorporadas em sementes artificiais para avaliação de sua toxicidade a C. maculatus. As fracões mais ácidas de ambas vicilinas afetaram o desenvolvimento e a sobrevivência do bruquídeo. Sugerimos que polipeptídeos de vicilinas de natureza tóxica são expressos em ambos tipos de globulinas de reserva, embora em níveis diferentes.

  16. Typhlocharis vicariantes del Estrecho de Gibraltar. I: Typhlocharis armata Coiffait, 1969 (Coleoptera, Caraboidea, Trechidae

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    Pérez González, S.

    2010-12-01

    Full Text Available This first part about the vicariant Typhlocharis from Gibraltar Strait is dedicated to Typhlocharis armata Coiffait, 1969; a second paper will cover T. silvanoides Dieck, 1869. The studied material was obtained in four field campaigns carried out in the south of Spain and north of Morocco between 1997 and 2001 (Zaballos & Banda, 2000; Zaballos, 2005. In these campaigns abundant specimens of Typhlocharis (Coleoptera, Caraboidea, Trechidae were collected in different localities, the majority of them belonging to the species T. armata Coiffait, 1969. Their study and the comparison with the type series of this species allowed its complete redescription and clarification of its status within the genus. Contrary to what has been considered traditionally, the marginal umbilicate series of elytra in T. armata is composed of three, instead of four posterior setae. The taxonomical affinities of this species are established, the morphological weight of the umbilicate group of elytral setae as a lineage and species group defining character is discussed, and the theory of Jeanne (1973 about the relation between shortening of elytra and umbilicate series reduction with a decrease in body size is revised. The dispersal abilities are evaluated and a biogeographical explanation for the new distribution of the species is proposed.Esta primera parte sobre las especies de Typhlocharis vicariantes del Estrecho de Gibraltar está dedicada a Typhlocharis armata Coiffait, 1969; mientras que en un segundo artículo se tratará T. silvanoides Dieck, 1869. El material estudiado procede de cuatro campañas entomológicas realizadas en el sur de España y en el norte de Marruecos entre 1997 y 2001 (Zaballos & Banda, 2000; Zaballos, 2005. En estas campañas se recolectaron, en localidades diferentes, abundantes ejemplares de Typhlocharis (Coleoptera, Caraboidea, Trechidae, la mayoría de los cuales han resultado pertenecer a la especie T. armata Coiffait, 1969. Su estudio y

  17. Morphological and genetic variation in Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae, Cicindelinae: implications for conservation

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    Serrano, A.

    2003-12-01

    Full Text Available The establishment of taxonomic and geographical boundaries is a common problem when analysing clinal distributions. This is of particular concern when the assessment of intraspecific groupings is required for conservation management. The tiger beetle Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae is a typical case in which two recognised subspecies are distributed in a clinal latitudinal fashion in the dune systems along the Atlantic coast of Portugal. This habitat is increasingly under threat, and conservation measures are needed. We investigated the validity of the two named subspecies, based on a re-analysis of elytral and genitalic measurements using multivariate analysis. We also analysed variation in mitochondrial Cytochrome Oxidase I gene for a total of six populations along the cline. Multivariate analysis supported the idea of a morphological cline and revealed a clear distinction of the southernmost population and also some degree of distinctiveness of the most northern populations, partially supporting the recognised subspecific ranking. The mtDNA analysis identified two main groups corresponding to northern and southern populations. Both sets of markers showed that variation within the C. lusitanica assemblage is complex, with the boundaries between morphological and mtDNA groups not in agreement. However, populations at either end of the distributional range are clearly distinct from each other, and should be considered as provisional units for conservation programmes.El reconocimiento de límites taxonómicos y geográficos de la variabilidad observada es un problema habitual cuando se analizan distribuciones clinales. Esto es particularmente problemático cuando se requiere la determinación de agrupamientos intraespecíficos para tomar medidas de conservación. El cicindélido Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae constituye un caso típico en el que dos subespecies reconocidas se hayan distribuidas a

  18. Observations on sex ratio and behavior of males in Xyleborinus saxesenii Ratzeburg (Scolytinae, Coleoptera

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    Peter Biedermann

    2010-09-01

    Full Text Available Strongly female-biased sex ratios are typical for the fungal feeding haplodiploid Xyleborini (Scolytinae, Coleoptera, and are a result of inbreeding and local mate competition (LMC. These ambrosia beetles are hardly ever found outside of trees, and thus male frequency and behavior have not been addressed in any empirical studies to date. In fact, for most species the males remain undescribed. Data on sex ratios and male behavior could, however, provide important insights into the Xyleborini’s mating system and the evolution of inbreeding and LMC in general. In this study, I used in vitro rearing methods to obtain the first observational data on sex ratio, male production, male and female dispersal, and mating behavior in a xyleborine ambrosia beetle. Females of Xyleborinus saxesenii Ratzeburg produced between 0 and 3 sons per brood, and the absence of males was relatively independent of the number of daughters to be fertilized and the maternal brood sex ratio. Both conformed to a strict LMC strategy with a relatively precise and constant number of males. If males were present they eclosed just before the first females dispersed, and stayed in the gallery until all female offspring had matured. They constantly wandered through the gallery system, presumably in search of unfertilized females, and attempted to mate with larvae, other males, and females of all ages. Copulations, however, only occurred with immature females. From galleries with males, nearly all females dispersed fertilized. Only a few left the natal gallery without being fertilized, and subsequently went on to produce large and solely male broods. If broods were male-less, dispersing females always failed to found new galleries.

  19. Diel Patterns of Colaspis brunnea and Colaspis crinicornis (Coleoptera: Chrysomelidae) in Southeastern Nebraska.

    Science.gov (United States)

    Miwa, Kentaro; Meinke, Lance J

    2015-12-01

    A field study was conducted to increase our understanding of diel activity patterns of Colaspis brunnea (F.) and Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) in key crop habitats. Within 24-h periods, C. brunnea was sampled in clover fields (primarily red clover, Trifolium pretense (L.), with some sweet clover, Melilotus officinalis (L.) Pallas, and downy brome, Bromus tectorum (L.)) and soybean, Glycine max (L.) Merrill, fields, using a sweep-net, while whole-plant-count sampling was used to monitor C. crinicornis densities in field corn, Zea mays (L.). Sweep-net captures of C. brunnea were significantly greater at night than during the day, suggesting possible vertical movement within the canopy during a 24-h period. Colaspis crinicornis densities on corn plants were fairly constant throughout a 24-h period, but beetle activity (e.g., walking, mating) was significantly greater at night than during the day. Results suggest that both Colaspis species may be exhibiting similar increases in activity at night that facilitates movement from more protected to more exposed areas within a habitat. It is unclear what mechanisms drive this diel pattern, but vegetation architecture and associated interactions with environmental conditions may play a role. Sweep-netting in clover or soybean fields and use of whole-plant-counts in cornfields were effective sampling methods for Colaspis adults. However, because activity and behaviors of Colaspis beetles were influenced by time of day in this study, use of a consistent sampling time within a diel period would be recommended for future population studies or integrated pest management decision-making. PMID:26314034

  20. Organization and comparative analysis of the mitochondrial genomes of bioluminescent Elateroidea (Coleoptera: Polyphaga).

    Science.gov (United States)

    Amaral, Danilo T; Mitani, Yasuo; Ohmiya, Yoshihiro; Viviani, Vadim R

    2016-07-25

    Mitochondrial genome organization in the Elateroidea superfamily (Coleoptera), which include the main families of bioluminescent beetles, has been poorly studied and lacking information about Phengodidae family. We sequenced the mitochondrial genomes of Neotropical Lampyridae (Bicellonycha lividipennis), Phengodidae (Brasilocerus sp.2 and Phrixothrix hirtus) and Elateridae (Pyrearinus termitilluminans, Hapsodrilus ignifer and Teslasena femoralis). All species had a typical insect mitochondrial genome except for the following: in the elaterid T. femoralis genome there is a non-coding region between NADH2 and tRNA-Trp; in the phengodids Brasilocerus sp.2 and P. hirtus genomes we did not find the tRNA-Ile and tRNA-Gln. The P. hirtus genome showed a ~1.6kb non-coding region, the rearrangement of tRNA-Tyr, a new tRNA-Leu copy, and several regions with higher AT contents. Phylogenetics analysis using Bayesian and ML models indicated that the Phengodidae+Rhagophthalmidae are closely related to Lampyridae family, and included Drilus flavescens (Drilidae) as an internal clade within Elateridae. This is the first report that compares the mitochondrial genomes organization of the three main families of bioluminescent Elateroidea, including the first Neotropical Lampyridae and Phengodidae. The losses of tRNAs, and translocation and duplication events found in Phengodidae mt genomes, mainly in P. hirtus, may indicate different evolutionary rates in these mitochondrial genomes. The mitophylogenomics analysis indicates the monophyly of the three bioluminescent families and a closer relationship between Lampyridae and Phengodidae/Rhagophthalmidae, in contrast with previous molecular analysis. PMID:27060405

  1. The presence of a mycangium in European Sinodendron cylindricum (Coleoptera: Lucanidae) and the associated yeast symbionts

    Science.gov (United States)

    Tanahashi, Masahiko; Hawes, Colin J.

    2016-01-01

    Part of the exoskeleton of some wood-inhabiting insects is modified to form a mycangium, which is a specialized organ used to convey fungal spores or yeasts to their offspring. Although most stag beetles (Coleoptera: Lucanidae) are known to have female-specific mycangia and associated yeast symbionts, the evolutionary origin of the mycangium in this group remains unresolved. Here, we report the presence of a mycangium and associated yeast symbionts in the European horned stag beetle Sinodendron cylindricum (L.), which belongs to an ancestral clade of the Lucanidae. The mycangium of S. cylindricum is shown to be female-specific and have the same developmental origin as that of other stag beetles. A total of five yeast strains were isolated from adult mycangia and larval gut of S. cylindricum. Of these, we suggest that SICYAM1 is an undescribed yeast with taxonomic novelty, and have identified SICYLG3 as the xylose-fermenting yeast Scheffersomyces insectosa using nuclear ribosomal RNA and ITS sequences. The remaining three yeast strains, SICYAM2, SICYLG1, and SICYLG2, were assigned to the genus Sugiyamaella. Yeast density in the adult mycangium was lower than that of the more evolutionarily advanced stag beetles, the European Lucanus cervus (L.) and Dorcus parallelipipedus (L.), which were also examined in this study. No living yeasts were isolated from the adult guts. However, a third instar larva of S. cylindricum harbored 104–106 living yeasts in each gut region, which suggests that gut yeasts play an important role in these wood-feeding larvae. PMID:27432353

  2. Relationships of abscised cotton fruit to boll weevil (Coleoptera: Curculionidae) feeding, oviposition, and development.

    Science.gov (United States)

    Showler, Allan T

    2008-02-01

    Abscised cotton, Gossypium hirsutum L., fruit in field plots planted at different times were examined to assess adult boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), use of squares and bolls during 2002 and 2003 in the Lower Rio Grande Valley of Texas. Although boll abscission is not necessarily related to infestation, generally more bolls abscised than squares and abundances of fallen bolls were not related to the planting date treatments. During 2003, fallen squares were most abundant in the late-planted treatment. Although large squares (5.5-8-mm-diameter) on the plant are preferred for boll weevil oviposition, diameter of abscised squares is not a reliable measurement because of shrinkage resulting from desiccation and larval feeding. Fallen feeding-punctured squares and bolls were most abundant in late plantings but differences between fallen feeding-punctured squares versus fallen feeding-punctured bolls were found in only one treatment in 2003. During the same year, fallen oviposition-punctured squares were more numerous in the late-planted treatment than in the earlier treatments. Treatment effects were not found on numbers of oviposition-punctured bolls, but fallen oviposition-punctured squares were more common than bolls in the late-planted treatment compared with earlier treatments each year. Dead weevil eggs, larvae, and pupae inside fallen fruit were few and planting date treatment effects were not detected. Living third instars and pupae were more abundant in fallen squares of the late-planted treatment than in the earlier treatments and bolls of all three treatments. This study shows that fallen squares in late-planted cotton contribute more to adult boll weevil populations than bolls, or squares of earlier plantings. PMID:18330118

  3. Influence of trap color and host volatiles on capture of the emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Crook, Damon J; Khrimian, Ashot; Cossé, Allard; Fraser, Ivich; Mastro, Victor C

    2012-04-01

    Field trapping assays were conducted in 2009 and 2010 throughout western Michigan, to evaluate lures for adult emerald ash borer, A. planipennis Fairmaire (Coleoptera: Buprestidae). Several ash tree volatiles were tested on purple prism traps in 2009, and a dark green prism trap in 2010. In 2009, six bark oil distillate lure treatments were tested against manuka oil lures (used in 2008 by USDA APHIS PPQ emerald ash borer cooperative program). Purple traps baited with 80/20 (manuka/phoebe oil) significantly increased beetle catch compared with traps baited with manuka oil alone. In 2010 we monitored emerald ash borer attraction to dark green traps baited with six lure combinations of 80/20 (manuka/phoebe), manuka oil, and (3Z)-hexenol. Traps baited with manuka oil and (3Z)-hexenol caught significantly more male and total count insects than traps baited with manuka oil alone. Traps baited with manuka oil and (3Z)-hexenol did not catch more beetles when compared with traps baited with (3Z)-hexenol alone. When compared with unbaited green traps our results show that (3Z)-hexenol improved male catch significantly in only one of three field experiments using dark green traps. Dark green traps caught a high number of A. planipennis when unbaited while (3Z)-hexenol was seen to have a minimal (nonsignificant) trap catch effect at several different release rates. We hypothesize that the previously reported kairomonal attractancy of (3Z)-hexenol (for males) on light green traps is not as obvious here because of improved male attractancy to the darker green trap. PMID:22606813

  4. Displacement of Tetropium cinnamopterum (Coleoptera: Cerambycidae) by Its Invasive Congener Tetropium fuscum.

    Science.gov (United States)

    Dearborn, Kenneth W; Heard, Stephen B; Sweeney, Jon; Pureswaran, Deepa S

    2016-08-01

    We examined the native community of insects interacting with an invasive species, Tetropium fuscum (F.) (Coleoptera: Cerambycidae), in its new range to explore reasons for the invader's relatively slow spread. Tetropium fuscum is a European spruce borer established in Nova Scotia since at least 1990, but it has spread only about 125 km from its site of introduction. We compared the densities of Tetropium spp., their known parasitoids, and the community of wood-boring insects at sites located within the invasion zone in Nova Scotia versus well outside this zone, in New Brunswick, Canada. Using red spruce trees stressed by girdling or felling, we tested whether: 1) T. fuscum had altered the native wood-boring community; 2) T. fuscum displaced a native congener, Tetropium cinnamopterum (Kirby); and 3) parasitism rates of Tetropium spp. differed between the invaded and noninvaded zones. Both Tetropium spp. and their parasitoid wasps emerged exclusively from felled trees as opposed to girdled trees. We found no difference in community diversity inside versus outside the invasion zone. The combined densities of both Tetropium spp. and their overall parasitism rates also did not differ between zones, but T. cinnamopterum density was significantly greater outside the invasion zone, suggesting T. fuscum may displace the native congener where they are sympatric. Our results suggest that the native and invasive Tetropium spp. act as a single functional species in the invasion zone. We speculate that natural control agents (predators, parasitoids, and competitors) might be limiting the rate of spread of T. fuscum. PMID:27247305

  5. Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae of an Ecuadorian Mountain Forest Using DNA Barcoding.

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    Birthe Thormann

    Full Text Available Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient in southern Ecuador and explore their effect on species richness estimates.Based on a COI barcode data set of 674 leaf beetle specimens (Coleoptera: Chrysomelidae of 266 morphospecies from three sample sites in the Podocarpus National Park, we employed statistical parsimony analysis, distance-based clustering, GMYC- and PTP-modelling to delimit species-like units and compared them to morphology-based (parataxonomic species identifications. The four different approaches for DNA-based species delimitation revealed highly similar numbers of molecular operational taxonomic units (MOTUs (n = 284-289. Estimated total species richness was considerably higher than the sampled amount, 414 for morphospecies (Chao2 and 469-481 for the different MOTU types. Assemblages at different elevational levels (1000 vs. 2000 m had similar species numbers but a very distinct species composition for all delimitation methods. Most species were found only at one elevation while this turnover pattern was even more pronounced for DNA-based delimitation.Given the high congruence of DNA-based delimitation results, probably due to the sampling structure, our study suggests that when applied to species communities on a regionally limited level with high amount of rare species (i.e. ~50% singletons, the choice of species delimitation method can be of minor relevance for assessing species numbers and turnover in tropical insect communities. Therefore, DNA-based species delimitation is confirmed as a

  6. Dung beetle assemblages (Coleoptera, Scarabaeinae in Atlantic forest fragments in southern Brazil

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    Renata C. Campos

    2013-03-01

    Full Text Available Dung beetle assemblages (Coleoptera, Scarabaeinae in Atlantic forest fragments in southern Brazil. The beetles of the subfamily Scarabaeinae are important organisms that participate in the cycle of decomposition, especially in tropical ecosystems. Most species feed on feces (dung or carcasses (carrion and are associated with animals that produce their food resources. Dung beetles are divided into three functional groups: rollers, tunnelers and dwellers. This present work aims to study the diversity of dung beetle communities inhabiting fragments of the Atlantic Forest, with the purpose of describing the ecology of the species in southern Brazil. This study was conducted in the region of Campos Novos, in Santa Catarina, where twenty sites of Atlantic forest fragments were sampled. Samplings of dung beetles were conducted using 200 pitfall traps, of which 100 were baited with human feces and another 100 with carrion. Size and environmental complexity were also measured for each forest fragment. A total of 1,502 dung beetles, belonging to six tribes, 12 genera and 33 species, were collected. Results of the Levin's index of niche breadth indicated that 11 species were categorized as being coprophagous, ten as generalists, and two as necrophagous. Most species are tunnelers (19, nine of rollers and four of dwellers. The great diversity of Scarabaeinae in the region of Campos Novos, including several rare species, adds important data to the Scarabaeinae fauna in the central-western region of Santa Catarina. It may also help choosing priority areas for conservation in the region, where human impact, with large areas of monoculture, increasingly threatens the fragments of Mixed Ombrophilous Forest.

  7. Scarab Beetle (Coleoptera: Scarabaeidae Fauna in Ardabil Province, North West Iran

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    G Mowlavi

    2008-12-01

    Full Text Available Background: Dung beetles of Coleoptera associated to undisturbed cattle droppings in pastures present great diver¬sity and abundance. Dung beetles also play an important role for transmission of some helminthes to human and cat¬tle. This study was made to survey the biodiversity and abundance of these beetles in Ardebil Province, western Iran.Methods: According to the field study all beetles attracted to fresh cow dung in five areas of Ardebil Province in¬cluding Namin, Ardabil, Meshkinshahr, Neer and Sarein were collected and identified. They were collected during summer 2007 from June to September, with general peaks appearing to be correlated with temperature mainly at 11 a.m to 15 p.m. The samples were identified using appropriate systematic key Results: A total of 231 specimens belonging to 9 beetle genera and at least 15 species were identified as Euoniticel¬lus fulvus, Sisyphus schaffaer, Euonthophagus taurus, Copris lunaris, Chironitis pamphilus, Gymnopleurus coriarus, Euonthophagus amyntas, Caccobius schreberi, Onthophagus speculifer, Onthophagus furcatus, Aphodius, lugens, Apho¬dius fimetarius, A. scrutator, Geotrupes spiniger and G. stercorariusThe most abundant and diverse subfamilies were Coprinae, Geotrupinae, and Aphodiinae. Conclusion: We found 15 species of dung beetles occurred in the region. The prevalence of each species is varied depending on location. Some of them play an important role for helminths transmission of veterinary and public health importance. The finding will provide a clue for pasture management as well as public health monitoring and surveillance of the disease transmitted by dung beetles. 

  8. Scarab Beetle (Coleoptera: Scarabaeidae Fauna in Ardabil Province, North West Iran

    Directory of Open Access Journals (Sweden)

    G Mowlavi

    2008-12-01

    Full Text Available "nBackground: Dung beetles of Coleoptera associated to undisturbed cattle droppings in pastures present great diver¬sity and abundance. Dung beetles also play an important role for transmission of some helminthes to human and cat¬tle. This study was made to survey the biodiversity and abundance of these beetles in Ardebil Province, western Iran."nMethods: According to the field study all beetles attracted to fresh cow dung in five areas of Ardebil Province in¬cluding Namin, Ardabil, Meshkinshahr, Neer and Sarein were collected and identified. They were collected during summer 2007 from June to September, with general peaks appearing to be correlated with temperature mainly at 11 a.m to 15 p.m. The samples were identified using appropriate systematic key "nResults: A total of 231 specimens belonging to 9 beetle genera and at least 15 species were identified as Euoniticel¬lus fulvus, Sisyphus schaffaer, Euonthophagus taurus, Copris lunaris, Chironitis pamphilus, Gymnopleurus coriarus, Euonthophagus amyntas, Caccobius schreberi, Onthophagus speculifer, Onthophagus furcatus, Aphodius, lugens, Apho¬dius fimetarius, A. scrutator, Geotrupes spiniger and G. stercorarius"nThe most abundant and diverse subfamilies were Coprinae, Geotrupinae, and Aphodiinae. "nConclusion: We found 15 species of dung beetles occurred in the region. The prevalence of each species is varied depending on location. Some of them play an important role for helminths transmission of veterinary and public health importance. The finding will provide a clue for pasture management as well as public health monitoring and surveillance of the disease transmitted by dung beetles. 

  9. A comparison of trap type and height for capturing cerambycid beetles (Coleoptera).

    Science.gov (United States)

    Graham, Elizabeth E; Poland, Therese M; McCullough, Deborah G; Millar, Jocelyn G

    2012-06-01

    Wood-boring beetles in the family Cerambycidae (Coleoptera) play important roles in many forest ecosystems. However, increasing numbers of invasive cerambycid species are transported to new countries by global commerce and threaten forest health in the United States and worldwide. Our goal was to identify effective detection tools for a broad array of cerambycid species by testing some known cerambycid attractants and a pheromone in different trap designs placed across a range of habitats. We compared numbers and species richness of cerambycid beetles captured with cross-vane panel traps and 12-unit Lindgren multiple-funnel traps, placed either at ground level (1.5 m high) or canopy level (approximately 3-10 m high), at eight sites classified as either residential, industrial, deciduous forest, or conifer forest. We captured 3,723 beetles representing 72 cerambycid species from 10 June to 15 July 2010. Species richness was highest for the subfamilies Cerambycinae and Lamiinae, which accounted for 33 and 46% of all species captured, respectively. Overall, the cross-vane panel traps captured approximately 1.5 times more beetles than funnel traps. Twenty-one species were captured exclusively in traps at one height, either in the canopy or at ground level. More species were captured in hardwood sites (59 species) where a greater diversity of host material was available than in conifer (34 species), residential (41 species), or industrial (49) sites. Low numbers of beetles (n < 5) were recorded for 28 of the beetle species. The number of species captured per week ranged from 49 species on 21 June to 37 species on 12 July. Cross-vane panel traps installed across a vertical gradient should maximize the number of cerambycid species captured. PMID:22812119

  10. Tropical Plant Extracts as Sources of Grain-Protectant Compounds Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae).

    Science.gov (United States)

    Ribeiro, L P; Vendramim, J D; Andrade, M S; Bicalho, K U; Silva, M F G F; Vieira, P C; Fernandes, J B

    2014-10-01

    The toxicity of organic plant extracts to Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) was assessed for three tropical plant species: branches, leaves, and seeds of Annona montana (Annonaceae), branches of Aristolochia paulistana (Aristolochiaceae), and leaves and branches of Casearia sylvestris (Salicaceae). The screening assay resulted that the extracts of A. montana seeds obtained with hexane (LC50 = 534.75 mg kg(-1) and LT50 = 6.10 days) and with dichloromethane (LC50 = 424.67 mg kg(-1) and LT50 = 5.03 days) were the most promising treatments, followed by the extract prepared from A. montana leaves with hexane (LC50 = 837.70 mg kg(-1) and LT50 = 4.90 days). Moreover, extracts (at 1,500 mg kg(-1)) prepared from C. sylvestris branches with dichloromethane and A. paulistana with hexane caused significant mortality (37% and 41.5%, respectively) beyond sublethal effects on S. zeamais. Therefore, based on the biological assays, extraction yield, and evaluation of the chromatographic profile of the crude extracts by TLC, the hexane extract of A. montana seeds was selected and fractioned using liquid-liquid partitioning. The hydroalcoholic fraction caused mortality of 55.5%, significantly superior to dichloromethane fraction, which caused 35.5% of mortality. Chemical analyses ((1)H NMR, HPLC, and TLC) were performed, and the results showed the presence of alkaloids and acetogenins in both active fractions, which have been associated with bioactivity. Therefore, extracts prepared from A. montana seeds (especially) is an interesting source of new compounds with promising grain-protectant properties. PMID:27193958

  11. Variability in Small Hive Beetle (Coleoptera: Nitidulidae) Reproduction in Laboratory and Field Experiments.

    Science.gov (United States)

    Meikle, William G; Holst, Niels; Cook, Steven C; Patt, Joseph M

    2015-06-01

    Experiments were conducted to examine how several key factors affect population growth of the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae). Laboratory experiments were conducted to examine effects of food quantity and temperature on reproduction of cohorts of young A. tumida adults (1:1 sex ratio) housed in experimental arenas. Daily numbers and total mass of larvae exiting arenas were highly variable within treatment. Either one or two cohorts of larvae were observed exiting the arenas. Food quantity, either 10 g or 20 g, did not significantly affect the number of larvae exiting arenas at 32°C, but did at 28°C; arenas provided 20 g food produced significantly more larvae than arenas provided 10 g. Temperature did not affect the total mass of larvae provided 10 g food, but did affect larval mass provided 20 g; beetles kept at 28°C produced more larval mass than at 32°C. Field experiments were conducted to examine A. tumida reproductive success in full strength bee colonies. Beetles were introduced into hives as egg-infested frames and as adults, and some bee colonies were artificially weakened through removal of sealed brood. Efforts were unsuccessful; no larvae were observed exiting from, or during the inspection of, any hives. Possible reasons for these results are discussed. The variability observed in A. tumida reproduction even in controlled laboratory conditions and the difficulty in causing beetle infestations in field experiments involving full colonies suggest that accurately forecasting the A. tumida severity in such colonies will be difficult. PMID:26470208

  12. Comparative resistance of Russian and Italian honey bees (Hymenoptera: Apidae) to small hive beetles (Coleoptera: Nitidulidae).

    Science.gov (United States)

    Frake, Amanda M; De Guzman, Lilia I; Rinderer, Thomas E

    2009-02-01

    To compare resistance to small hive beetles (Coleoptera: Nitidulidae) between Russian and commercial Italian honey bees (Hymenoptera: Apidae), the numbers of invading beetles, their population levels through time and small hive beetle reproduction inside the colonies were monitored. We found that the genotype of queens introduced into nucleus colonies had no immediate effect on small hive beetle invasion. However, the influence of honey bee stock on small hive beetle invasion was pronounced once test bees populated the hives. In colonies deliberately freed from small hive beetle during each observation period, the average number of invading beetles was higher in the Italian colonies (29 +/- 5 beetles) than in the Russian honey bee colonies (16 +/- 3 beetles). A similar trend was observed in colonies that were allowed to be freely colonized by beetles throughout the experimental period (Italian, 11.46 +/- 1.35; Russian, 5.21 +/- 0.66 beetles). A linear regression analysis showed no relationships between the number of beetles in the colonies and adult bee population (r2 = 0.1034, P = 0.297), brood produced (r2 = 0.1488, P = 0.132), or amount of pollen (P = 0.1036, P = 0.295). There were more Italian colonies that supported small hive beetle reproduction than Russian colonies. Regardless of stock, the use of entrance reducers had a significant effect on the average number of small hive beetle (with reducer, 16 +/- 3; without reducer, 27 +/- 5 beetles). However, there was no effect on bee population (with reducer, 13.20 +/- 0.71; without reducer, 14.60 +/- 0.70 frames) or brood production (with reducer, 6.12 +/- 0.30; without reducer, 6.44 +/- 0.34 frames). Overall, Russian honey bees were more resistant to small hive beetle than Italian honey bees as indicated by fewer invading beetles, lower small hive beetle population through time, and lesser reproduction. PMID:19253612

  13. Endophyte isolate and host grass effects on Chaetocnema pulicaria (Coleoptera: Chrysomelidae) feeding.

    Science.gov (United States)

    Ball, Olivier J P; Gwinn, Kimberly D; Pless, Charles D; Popay, Alison J

    2011-04-01

    Endophytic fungi belonging to the genus Neotyphodium, confer resistance to infected host grasses against insect pests. The effect of host species, and endophtye species and strain, on feeding and survival of the corn flea beetle, Chaetocnema pulicaria Melsheimer (Coleoptera: Chrysomelidae) was investigated. The grass-endophyte associations included natural and artificially derived associations producing varying arrays of common endophyte-related alkaloids or alkaloid groups, peramine, lolitrem B, ergovaline, and the lolines. Preference and nonpreference tests showed that C. pulicaria feeding and survival were reduced by infection of tall fescue with the wild-type strain of N. coenophialum, the likely mechanism being antixenosis rather than antibiosis. In the preference tests, endophyte and host species effects were observed. Of the 10 different Neotyphodium strains tested in artificially derived tall fescue associations, eight strongly deterred feeding by C. pulicaria, whereas the remaining two strains had little or no effect on feeding. Infection of tall fescue with another fungal symbiont, p-endophyte, had no effect. Perennial ryegrass, Lolium perenne L., infected with six strains of endophyte, was moderately resistant to C. pulicaria compared with endophyte-free grass, but four additional strains were relatively inactive. Six Neotyphodium-meadow fescue, Festuca pratensis Huds., associations, including the wild-type N. uncinatum-meadow fescue combination, were resistant, whereas three associations were not effective. Loline alkaloids seemed to play a role in antixenosis to C. pulicaria. Effects not attributable to the lolines or any other of the alkaloids examined also were observed. This phenomenon also has been reported in tests with other insects, and indicates the presence of additional insect-active factors. PMID:21510220

  14. Efficacy of natural products against Callosobruchus chinensis (Coleoptera: Bruchidae) in Nepal.

    Science.gov (United States)

    Regmi, Homan; Kafle, Lekhnath; Gc, Yubak Dhoj; Shih, Cheng Jen

    2012-06-01

    This study was conducted to determine the efficacy of local natural products against the beetle, Callosobruchus chinensis L. (Coleoptera: Bruchidae), in stored chickpea Cicer arietinum L. (Fabaceae) in Chitwan, Nepal. Five natural products and one synthetic pesticide (Malathion) and two storage regimes (aluminum sheet bin vs. jute bags with plastic lining) were tested for their effect on stored pulse with respect to damage by C. chinensis. The five natural products included Xanthoylum armatum DC (Rutaceae) fruit powder, Acorus calamus L. (Araceae) rhizome powder, Cinnamom camphora L. (Lauraceae) balls, oil of Sesamum indicum L. (Pedaliaceae), and leaf powder of Azadirachta indica A. Juss. (Meliaceae). Treatments of stored pulses with natural products or with Malathion all caused significantly higher mortality of C. chinensis at 15 d after treatment (DAT) than stored pulses receiving no treatments. The balls of C. camphora, rhizome powder of A. calamus and sesame oil outperformed all other treatments, including the Malathion at 45 and 75 DAT and resulted in significantly reduced damage or deterioration of stored pulses compared with other treatments. Storage regimes performed similarly, although the jute bags did protect seed integrity for some treatments. Our results indicate that incorporating these natural products into stored pulses can protect the seeds from C. chinensis for up to two generations, something that Malathion cannot do. These products are readily available to most farmers in the region and their use will lead to 1) reduction of losses to significant stored product pests, and 2) a reduction of contamination of foodstuffs and the environment by synthetic pesticides like Malathion. PMID:22812152

  15. Mortality of a wireworm, Agriotes obscurus (Coleoptera: Elateridae), after topical application of various insecticides.

    Science.gov (United States)

    Van Herk, W G; Vernon, R S; Tolman, J H; Saavedra, H Ortiz

    2008-04-01

    Ten insecticides representing seven chemical groups were applied at various concentrations topically by using a Potter Spray Tower to evaluate their relative toxicities on the European wireworm Agriotes obscurus L. (Coleoptera: Elateridae). Wireworms were stored at 15 degrees C after exposure to organophosphate (OP) (chlorpyrifos, diazinon), pyrethroid (tefluthrin), thianicotinoid (thiamethoxam, clothianidin), chloronicotinoid (imidacloprid, acetamiprid), phenyl pyrazole (fipronil), organochlorine (lindane), and spinosyn (spinosad) insecticides, and their postapplication health was evaluated weekly for up to 301 d. LC50, LC90, LT50, and LT90 values were calculated for each chemical except acetamiprid, and compared with those of lindane, clothianidin, and chlorpyrifos. Wireworms exposed to OPs died or recovered more quickly (LT50 < 20 d, LT90 < 50 d), than those exposed to all other insecticides tested except tefluthrin (LT50 = 25.5 d, LT90 = 66.5 d). Wireworms exposed to sublethal concentrations of all neonicotinoids quickly became moribund after application but made a full recovery. Wireworms exposed to fipronil at concentrations near the LC90 value showed no intoxication symptoms for up to 35 d, and they did not recover after symptoms developed. For each chemical, increasing the concentration increased the time required for wireworms to recover but decreased the time required to kill wireworms. Fipronil was highly toxic to wireworms (LC50 = 0.0001%), but acetamiprid (LC50 = 1.82%), imidacloprid (LC50 = 0.83%), tefluthrin (LC50 = 0.23%), diazinon (LC50 = 0.54%), and spinosad (LC50 = 0.51%) were not. The toxicity of both clothianidin (LC50 = 0.07%) and thiamethoxam (LC50 = 0.17%) were similar to those oflindane (LC50 = 0.06%) and chlorpyrifos (LC50 = 0.10%). PMID:18459401

  16. Nutritional Physiology of the Khapra Beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae) Fed on Various Barley Cultivars.

    Science.gov (United States)

    Seifi, S; Naseri, B; Razmjou, J

    2016-02-01

    The Khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae), is known as one of the mostserious pests of grains in many parts of the world. In this study, the effect of nine barley cultivars (‘Bahman’,‘CB-84-10’, ‘Fajr 30’, ‘Makuyi’, ‘Nosrat’, ‘Yousof’, ‘13A1’, ‘18A1’, and ‘19 A1’) and a wheat cultivar (‘MV17’, as a control) was determined on the nutritional indices and digestive enzymatic activity of T. granarium at 33 6 1C,relative humidity of 6565%, and a photoperiod of 14:10 (L:D) h. The highest and lowest values of larval weight gain of sixth instar were detected on wheat (0.757±0.068 mg) and cultivar Bahman (0.342±0.071 mg). Also, T. granarium larvae fed on cultivar Bahman had the lowest value of efficiency of conversion of ingested food(10.90±2.09%) as compared with wheat and other barley cultivars. Also, the highest midgut amylolytic and proteolytic activities of sixth instar were on cultivar Bahman (0.364±0.024 mU/mg and 80.54±1.73 U/mg, respectively)and the lowest activities were on cultivar Nosrat (0.043±0.004 mU/mg and 7.15±0.01 U/mg, respectively).It is concluded that barley cultivar Bahman was the most unsuitable host for feeding of T. granarium. PMID:26612893

  17. Distribución y patrones de diversidad de los Afódidos en la Comunidad de Madrid (Coleoptera, Scarabaeoidea, Aphodiidae, Aphodiinae y Psammodiinae

    Directory of Open Access Journals (Sweden)

    Hortal, J.

    2006-05-01

    Full Text Available In this work we present an updated checklist of the Aphodiidae (Coleoptera, Scarabaeoidea from Comunidad de Madrid (Spain. In addition, the observed and potential distributions of the 70 species found in Madrid are mapped. The potential spatial distributions of species richness, rarity and endemism in this region are also mapped. Finally, we discuss briefly the origin of the observed patterns.

    En este trabajo se presenta un inventario actualizado de los Aphodiidae (Coleoptera, Scarabaeoidea de la Comunidad de Madrid. Además, se presentan mapas de la distribución observada y potencial de las 70 especies encontradas en Madrid, así como de la distribución espacial de la riqueza, rareza, y endemicidad potencial en la región. Finalmente, se discute brevemente el origen de los patrones observados.

  18. Mass Trapping and Classical Biological Control of Rhynchophorus palmarum L. 1794 (Coleoptera: Curculionidae. A hypothesis based in morphological evidences.

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    Flávio Miguens

    2011-07-01

    Resumo. Coleópteros têm sido descritos como pragas e vetores de nematódeos causadores de Anel Vermelho em diversas palmeiras da família Arecaceae. Rhynchophorus palmarum L (Coleoptera: Curculionidae é uma praga que afeta a cocoicultura e outras palmeiras; e, vetor de Bursaphelencus cocophilus (Cobb Baujard (Nematoda, agente etiológico de Anel Vermelho e de outros nematódeos. Atualmente, recomenda-se o emprego de inimigos naturais e parasitas no manejo integrado de pragas; dentre elas, Rhynchophorinae. Armadilhas de coleta massal são recomendadas no manejo integrado de pragas. Nosso estudo relata, na cocoicultura, a eficiência de armadilhas artesanais de baixo custo e a utilização cariomônios (toletes de cana-de-açúcar e cariômonios mais feromônios (toletes de cana-de-açúcar e machos adultos de R. palmarum como atrativos nas armadilhas. Ácaros ectoparasitas foram identificados nestes coleópteros, por microscopia, que podem ser propostos como parte do manejo integrado desta praga. Armadilhas de coleta massal com cariômonios foram eficientes na captura de R. palmarum e outros Curculionidae. No entanto, armadilhas de coleta massal com cariômonios e feromônios aumentaram a atratividade, em relação às primeiras, para este Coleoptera e Cosmopolites sordidus (Germar (Coleoptera: Curculionidae. Eventualmente, exemplares de R. palmarum apresentavam infecção fúngica. Ácaros ectoparasitas infestavam mais de 50% dos exemplares de R. palmarum. A microanatomia destes ácaros e sua interação com R. palmarum foi preliminarmente descrita. Todos os estágios do ciclo de vida destes ácaros foram identificados no compartimento dos élitros. As evidências morfológicas suportam a hipótese de que estes ácaros podem ser empregados no controle biológico de R. palmarum em um programa de manejo integrado.

  19. Notes on Diochus Erichson, Lissohypnus Casey, and Oxybleptes Smetana (Coleoptera: Staphylinidae) in Florida, including a description of a new species of Lissohypnus

    OpenAIRE

    Frank, J. Howard; Kelly, Sandor L.; Almquist, David T.

    2014-01-01

    The known range of Oxybleptes meridionalis Smetana (Coleoptera: Staphylinidae) is expanded in Florida, USA, from Indian River and Manatee counties to now include Brevard, Highlands, Orange, Seminole and Volusia. Oxybleptes davisi (Notman) is confi rmed to exist in Florida, with records from Leon, Liberty and Wakulla counties in the Panhandle, and Orange County in central Florida. Lissohypnus texanus Casey is newly reported from Florida. A new species, Lissohypnus fullertoni, is described from...

  20. Description of a new species of Plesioclytus Giesbert (Coleoptera: Cerambycidae: Cerambycinae) from Georgia, and transfer of the genus to Plesioclytini Wappes and Skelley, new tribe

    OpenAIRE

    Wappes, James E.; Skelley, Paul E.

    2016-01-01

    Plesioclytus morrisi Wappes and Skelley new species (Coleoptera: Cerambycidae) from the Ohoopee dune system in central Georgia is described with comments on the biology of the new species. The taxonomic placement of Plesioclytus Giesbert in the tribe Clytini is questioned as key characters are found to differ from the current characters used to define the tribe in the New World, resulting in its transfer to the newly erected Plesioclytini Wappes and Skelley new tribe, defined herein. Habitat ...

  1. A Temperature-Dependent Development Model for Willow Beetle Species (Coleoptera: Chrysomelidae) in Ireland: Simulation of Phenology/Voltinism in Response to Climate Change

    OpenAIRE

    Pollard, Ciaran P.

    2015-01-01

    Rising fossil fuel prices, energy security and adherence to existing European Union (EU) climate/energy policies means that Ireland must look towards alternative energy sources to meet future demand. Woody biomass in the form of short rotation coppice willow (SRCW) is considered a viable option. SRCW is vulnerable to damage by a range of diseases and pests however. The blue (Phratora vulgatissima) and brown (Galerucella lineola) willow beetles (Coleoptera: Chrysomelidae) are identified econom...

  2. Mortality and impaired development of Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae) induced by extract of sangra d’água Croton urucurana Baill (Euphorbiaceae).

    OpenAIRE

    Gabriel dos Santos Carvalho; Leonardo Santana da Silva; Luciana Barboza Silva; Mayra Layra dos Santos Almeida; Bruno Ettore Pavam; Marize Terezinha Lopes Pereira Peres

    2014-01-01

    Phaseolus vulgaris L., the green bean, is an important source of protein and minerals in the diet of the population. But its productivity is affected by the attack of numerous pests, from emergence to storage, Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae) being the major pest of stored beans. In the search for alternatives to chemical control of this insect and considering the potential biological activity of plant secondary metabolites, this study was conducted in order to evaluate...

  3. A new species of Drapetes Megerle (Coleoptera: Elateridae), with taxonomic summaries and a key to the species of northern North America

    OpenAIRE

    Johnson, Paul J.

    2016-01-01

    Seven species of Drapetes Megerle (Coleoptera: Elateridae) are recognized from northern North America. Drapetes chiricahua new species is described from southeastern Arizona and north-central New Mexico. Drapetes parallelus Van Dyke is a senior homonym of Drapetes paralellus Cobos by variant spelling, and a new synonym of Drapetes cylindricus Fall. Drapetes paralellus Cobos is replaced with Drapetes cobosi new name. Drapetes clarki Bonvouloir is not a North American species and is given a red...

  4. A simultaneous journal / wiki publication and dissemination of a new species description: Neobidessodes darwiniensis sp. n. from northern Australia (Coleoptera, Dytiscidae, Bidessini)

    OpenAIRE

    Lars Hendrich; Michael Balke

    2011-01-01

    Abstract Here, we describe a new Australian species in journal format and simultaneously open the description in a wiki format on the www.species-id.net. The wiki format will always link to the fixed original journal description of the taxon, however it permits future edits and additions to species' taxonomy and biology. The diving beetle Neobidessodes darwiniensis sp. n. (Coleoptera: Dytiscidae, Bidessini) is described based on a single female, collected in a rest pool of the Harriet Creek i...

  5. A scientific note on a new pest for European honeybees: first report of small hive beetle Aethina tumida, (Coleoptera: Nitidulidae) in Italy

    OpenAIRE

    Palmeri, Vincenzo; Scirtò, Giuseppe; Malacrinò, Antonino; Laudani, Francesca; Campolo, Orlando

    2015-01-01

    International audience The small hive beetle (SHB), Aethina tumida Murray(Coleoptera Nitidulidae), is an important pest of honeybeesin many parts of the world. The beetle is native tosub-Saharan Africa and was unintentionally introducedinto USA (1996), Australia (2001) and in Canada(2002) (Ellis and Munn, 2005; Neumann and Ellis2008). In Europe the SHB was discovered in 2005 ina consignment of queens imported from Texas (USA)into Portugal and destroyed by the Portuguese NationalVeterinaria...

  6. Plant compounds insecticide activity against Coleoptera pests of stored products Compostos de plantas com atividade inseticida a coleópteros-praga de produtos armazenados

    OpenAIRE

    Márcio Dionizio Moreira; Marcelo Coutinho Picanço; Luiz Cláudio de Almeida Barbosa; Raul Narciso Carvalho Guedes; Mateus Ribeiro de Campos; Gerson Adriano Silva; Júlio Cláudio Martins

    2007-01-01

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed...

  7. Biological and Morphological Aspects of Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), a Pest of Apulian Pinewoods in Southern Italy

    OpenAIRE

    Eustachio Tarasco; Oreste Triggiani

    2011-01-01

    Huge defoliations by Anoxia (Mesanoxia) matutinalis spp. matutinalis Castelnau, 1832 (Coleoptera, Scarabeidae) have been observed in recent years in the coastal pinewoods (Pinus halepensis, P. pinea) of the southern Apulia Region of Italy. Starting from adults collected in the field, insect breeding was conducted in the laboratory, following the life-cycle from ovideposition to larval development and emergence. Observations were performed in the laboratory and the field to study the different...

  8. A diversidade inventarial de Coleoptera (Insecta em uma paisagem antropizada do Bioma Araucária The inventory diversity of Coleoptera (Insecta of an anthropized landscape in the Biome Araucaria

    Directory of Open Access Journals (Sweden)

    Norma G. Ganho

    2005-12-01

    Full Text Available Como parte do Projeto PROVIVE, foram analisadas a riqueza de espécies, a composição taxonômica, a proporção de espécies raras e a constância taxonômica ao nível de família relacionada à riqueza de espécies, em comunidades de Coleoptera, em Vila Velha, Ponta Grossa, Paraná. Os dados foram obtidos a partir de coletas através de armadilha malaise no estrato do sub-bosque de cinco áreas com diferentes graus de intervenção antrópica, de setembro de 1999 a agosto de 2000. As 52 semanas de amostragem nas cinco áreas resultaram na coleta de 10.822 indivíduos de 1659 espécies. Todas as áreas apresentaram alta riqueza de espécies e diversidade, como indicado por vários índices. A área em estágio mais avançado de sucessão vegetal foi menos rica do que aquelas em estágio inicial/intermediária. De acordo com diferentes estimadores de riqueza de espécies, o número de espécies coletadas poderia aumentar de 22-123% com o aumento do esforço de coleta. As áreas menos conservadas foram mais ricas em espécies raras ("singletons", "doubletons" e únicas do que as mais conservadas. Nas cinco áreas houve uma constância taxonômica entre as famílias mais ricas (Curculionidae, Chrysomelidae, Cerambycidae, Staphylinidae, Mordelidae, Elateridae, Scarabaeidae, Coccinellidae e Tenebrionidae envolvendo 60% do total de espécies, como observado para a abundância de indivíduos. A existência de um padrão de constância taxonômica de famílias, quando considerados 60% da riqueza de espécies e/ou de abundância de indivíduos por local, poderá tornar mais fácil e rápido o estudo de comunidades de Coleoptera, habilitando a ordem a ser um táxon indicador de condições ambientais de áreas florestadas.The species richness, taxonomic composition, rare species, and taxonomic constancy at family level were studied in communities of Coleoptera in Vila Velha, Ponta Grossa, Paraná, as part of PROVIVE project. The data were gathered

  9. Attraction of Astylus variegatus (Germ. (Coleoptera: Melyridae by volatile floral attractants Atração de Astylus variegatus (Germ. (Coleoptera: Melyridae por atraentes florais voláteis

    Directory of Open Access Journals (Sweden)

    Maurício Ursi Ventura

    2007-06-01

    Full Text Available The beetle Astylus variegatus (Germ. (Coleoptera: Melyridae is frequently found in flowers feeding on pollen. Responses of A. variegatus to volatile floral attractants were studied in common beans (Phaseolus vulgaris L. fields. Traps originally designed to capture Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, consisted of plastic bottles (2 L with 150 holes (5-mm diameter yellow gold painted and containing inside a plastic strip (3.5 ´ 25 cm with Lagenaria vulgaris (L. powder (0.28% B cucurbitacin - feeding stimulant and arrestant for diabroticites sprayed with carbaril insecticide. Treatments consisted of 1,4-dimethoxybenzene (one or two dispensers per trap, 1,4-dimethoxybenze + indole, 1,4-dimethoxybenzene + cinnameldehyde and control. Volatile average release rates (over ten days was approximately 32 mg day-1 per dispenser under laboratory conditions. 1,4-dimethoxybenzene-lured traps caught significantly more beetles than the control, three and seven days after trap setting. Ten days after the onset of the experiment, there were no differences in number of beetles caught by treatments. Captures were higher in the 1,4-dimethoxybenzene + cinnamaldehyde treatment than in 1,4-dimethoxybenzene only in the first assessment. Adding indole to 1,4-dimethoxybenzene did not improve beetle captures.O besouro Astylus variegatus (Germ. (Coleoptera: Melyridae é freqüentemente encontrado em flores onde se alimenta de pólen. Respostas de A. variegatus a atraentes voláteis florais foram estudadas em campos de feijão, Phaseolus vulgaris L. Armadilhas, originalmente desenvolvidas para capturar Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, consistiram de garrafas plástica (2 L com 150 perfurações (5 mm de diâmetro pintadas com tinta amarelo ouro contendo no seu interior uma fita plástica (3,5 ´ 25,0 cm com pó seco de frutos de Lagenaria vulgaris (L. (0,28% de cucurbitacina B estimulante alimentar e arrestante para diabrotic

  10. Eficiência do inseticida clorpirifós no controle de larvas de Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae na cultura da batata Performance of the insectide chlorpyrifos to control Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae in potato crop

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Salles

    1999-06-01

    Full Text Available Foi avaliado o desempenho do inseticida clorpirifós no controle de larvas de Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, na cultura da batata. O experimento foi desenvolvido na safra de primavera, quando ocorre maior incidência de insetos pragas e, conseqüentemente, danos nos tubérculos de batata no Rio Grande do Sul. As diferentes dosagens e formulações dos inseticidas foram aplicadas no sulco de plantio, utilizando-se clorpirifós (2, 3 e 4 l i.a./ha de Lorsban 480 CE e 2 e 3kg i.a./ha de Lorsban 10 G e como padrão, forate (2kg i.a./ha de Granutox 50G. Os melhores controles ocorreram nos tratamentos com Granutox 50G, na dosagem de 2kg i.a./ha; seguido por Lorsban 10 G, 3 e 2kg i.a./ha e Lorsban 480 CE, 4 e 3l i.a./ha.A field trial was conducted to evaluate the performance of the insecticide chlorpyrifos in two formulations, emulsifiable and granular, for the control of Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae in potato. The field trial was conducted during the spring season when incidence of insect pests and, consequently, damage are high in Rio Grande do Sul state, Brazil. The different dosages and formulations of insecticides were applied in the planting furrow utilizing chlorpyrifos (2, 3 and 4l i.a./ha of Lorsban 480 CE and 2 and 3kg i.a./ha de Lorsban 10G and the standard phorate (2kg i.a./ha of Granutox 50G. Best field control performances were obtained with Granutox 50G, in the dosage of 2kg i.a./ha; Lorsban 10G, 3 and 2kg i.a./ha, followed by Lorsban 480 CE, 4 and 3l i.a./ha.

  11. Hexamermis paranaense new species (Nematoda, Mermithidae: a parasite of Diloboderus abderus (Coleoptera, Scarabaeidae in Argentina Hexamermis paranaense sp. nov. (Nematoda, Mermithidae: parasito de Diloboderus abderus (Coleoptera, Scarabaeidae en Argentina

    Directory of Open Access Journals (Sweden)

    María Fernanda Achinelly

    2008-12-01

    Full Text Available A new species, Hexamermis paranaense n. sp. (Nematoda, Mermithidae, a parasite of larvae of Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae is described and illustrated. This new species is characterized by amphids small, amphidial opening pocket-shaped, the anterior portion of the vagina muscularized and slightly protruding with a descending branch forming a loop before joining the uterus, and three rows of genital papillae: the ventrolateral divided in two rows with eight papillae in the outer row and with six papillae in the inner one; the ventral row with four pairs and one single preanal papillae, and with two pairs, a triplete, one pair, a single and one pair postanal papillae.Una nueva especie, Hexamermis paranaense sp. nov. (Nematoda, Mermithidae, parásito de larvas del gusano blanco, Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae, se describe e ilustra. Esta nueva especie se caracteriza por poseer anfidos pequeños, con la apertura en forma de bolsillo, parte anterior de la vagina muscular, con una rama que desciende y forma un lazo antes de la conexión al útero, tres hileras de papilas genitales: la hilera de posición ventro-lateral dividida en dos hileras con ocho papilas en la hilera externa y con seis papilas en la interna; la hilera ventral con una papila, seguida de cuatro pares, todas en posición preanal y dos pares, interrumpidos por tres papilas, seguida de un par, una simple, y un par, todas de posición postanal.

  12. Morphological and isozymic banding pattern study of white grubs (Coleoptera: Melolonthidae as pest of bark crop in mounth Merapi’s slope.

    Directory of Open Access Journals (Sweden)

    SUGIYARTO

    2008-07-01

    Full Text Available White grub (Coleoptera: Melolonthidae is a group of soil pest at any agrosystem., especially at Salak pondoh (Salacca zalacca (Gaert. Voss. crop. The characteristics of this specimen were very crucial to be studied in order to find the exact biocontrol. The aim of this research was to know the characteristics of white grubs (Melolonthidae: Coleoptera based on morphological and isozyme banding patterns. This research was conducted on August - November 2007 at Sleman and Magelang districts for the morphological purposes, while for the isozyme data were conducted at Sub Laboratory Biology, Central Laboratory of Sebelas Maret University Surakarta. Sample was taken by using stratified random sampling method, on five stations. Polyacrylamide gel electrophoresis (PAGE using the vertical type was taken to isozyme analysis. The enzyme used in this research were peroxidase and esterase to detect the isozyme banding patterns. The results showed that there was no morphological variation of white grubs (Melolonthidae: Coleoptera at salak pondoh agroecosystem in Mounth Merapi’s slope. Based on this character, there was one species of white grub found, i.e. Holotrichia javana. There was a genetic variation based on the variation of isozyme banding patterns.

  13. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    Science.gov (United States)

    Hönnicke, M. G.; Cusatis, C.; Rigon, L.; Menk, R.-H.; Arfelli, F.; Foerster, L. A.; Rosado-Neto, G. H.

    2010-08-01

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures ( Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  14. Predation potential of Chilocorus cacti (Coleoptera: Coccinellidae) to the prickly pear cacti pest Dactylopius opuntiae (Hemiptera: Dactylopiidae).

    Science.gov (United States)

    Flores, A; Olvera, H; Rodríguez, S; Barranco, J

    2013-08-01

    Functional response of the predator Chilocorus cacti (Linnaeus) (Coleoptera: Coccinellidae) on five densities of Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae) female adults was assessed under laboratory conditions. The searching efficiency of C. cacti significantly decreased as prey density increased. The logistic regression for the predator had a negative and significant linear parameter indicating a type II functional response. Non-linear regression for Holling predator equation estimated a handling time of 1.79 ± 0.129 h and attack rate coefficient of 0.1003 ± 0.030. Most of this handling time was because the predator spent a lot of time removing the waxy coating that protects adult females of D. opuntiae. Chilocorus cacti consumes females of D. opuntiae in their reproductive stage; therefore, it could be an effective natural enemy to suppress or regulate low density populations of D. opuntiae, preventing them to reach high densities. PMID:23949861

  15. Characterization of the proteases in the midgut of the xylophagous larvae of Oemona hirta (Coleoptera:Cerambycidae)

    Institute of Scientific and Technical Information of China (English)

    Brian David Shaw; John Tane Christeller

    2009-01-01

    The protein digestive capability oftbe larvae of the longhorn beetle (Oemona hirta,Coleoptera:Cerambycidae,Fabricius,1775) was investigated.This species feeds only on wood where there is a high proportion of vascular tissue.The pH of the midgut,the major digestive organ,was alkaline and protein hydrolysis was maximal at alkaline pH.Use of specific synthetic peptide substrates showed that the major protease activities were the endopeptidases,trypsin and chymotrypsin-like activity,and the exopeptidase,leucine aminopeptidase and the pH curves corresponded to that with protein substrate.Studies using a range ofsefine protease inhibitors as well as specific inhibitors ofmetalloproteases,cysteine proteases and aspartate proteases confirmed a serine protease-based digestive system similar to earlier reports of sapwood-feeding Cerambycids.Control of these insect pests using protease inhibitors is discussed.

  16. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    International Nuclear Information System (INIS)

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures (Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  17. The effects of temperature, diet, and other factors on development, survivorship, and oviposition of Aethina tumida (Coleoptera: Nitidulidae).

    Science.gov (United States)

    Meikle, William G; Patt, Joseph M

    2011-06-01

    Developmental rate and survivorship of small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), life stages were measured across different temperatures (21, 25, 28, 32 and 35 degrees C) and diets, which included natural and artificial pollen, honey, and bee pupae. Temperature affected hatch success, time to hatching, and larval growth. Eggs hatched in 61 h at 21 degrees C but in Beetles lived longer at 28 degrees C or lower but produced the most eggs per female, regardless of diet, at 32 degrees C. Beetle density influenced fecundity: beetles kept at three pairs per vial laid 6.7 times more eggs per female than those kept as single pairs. Overall, beetles fared best at 28-32 degrees C with mortality of all stages highest at 35 degrees C. PMID:21735891

  18. Genetic differentiation among populations of the beetle Bolitophagus reticulatus (Coleoptera: tenebrionidae) in a fragmented and a continuous landscape.

    Science.gov (United States)

    Knutsen, H; Rukke, B A; Jorde, P E; Ims, R A

    2000-06-01

    The effect of habitat fragmentation on genetic differentiation among local populations of the fungivorous beetle Bolitophagus reticulatus (Coleoptera: Tenebrionidae) was studied in two contrasting landscapes: one heavily fragmented with forest fragments of variable size surrounded by inhabitable agricultural fields, the other an old forest providing a continuous habitat. The genetic structure of the beetle within each of the two contrasting areas was investigated by means of protein electrophoresis, screening four polymorphic loci in 20 populations from each area. In both areas there were significant genetic differences among local populations, but on average differentiation in the fragmented area was three times greater than in the continuous one, strongly indicating a genetic isolation effect of habitat fragmentation. These genetic results are in accordance with previous studies on dispersal in this species. PMID:10886382

  19. Biological control agent of larger black flour beetles (Coleoptera: Tenebrionidae): a nuisance pest developing in cotton gin trash piles.

    Science.gov (United States)

    Nansen, Christian; Stokes, Bryan; James, Jacob; Porter, Patrick; Shields, Eilson J; Wheeler, Terry; Meikle, William G

    2013-04-01

    The larger black flour beetles, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), feeds on saprophytic fungi found in gin trash piles and occasionally becomes a nuisance pest in adjacent homes and businesses. The potential of Steinernema carpocapsae 'NY 001,' as a potential control agent of larger black flour beetle under experimental conditions was examined with particular reference to the importance of soil moisture content. Without prospects of insecticides being labeled for control of larger black flour beetle in gin trash, the data presented here support further research into applications of entomopathogenic nematodes underneath gin trash piles as a way to minimize risk of larger black flour beetle populations causing nuisance to nearby homes and businesses. PMID:23786050

  20. Food source and residual efficacy of chlorfenapyr on sealed and unsealed concrete

    Science.gov (United States)

    Adult Tribolium castaneum (Herbst), the red flour beetle, were exposed at 1 day, and 2, 4, and 6 weeks post-treatment on sealed and unsealed concrete arenas treated with chlorfenapyr at rates of 2.8, 6.9, 13.5, 20.6, 27.5 mg active ingredient/m2. Beetles were held either with or without flour, and a...

  1. Fumigant Action of Acrolein on Insects and Seed Viability

    OpenAIRE

    Ali Asghr Pourmirza

    2007-01-01

    In laboratory experiments toxicity of acrolein vapors was investigated against 4 species of stored-product insects. In empty-space trials, estimated of the median lethal dosages of acrolein against adults of Oryzaephilus surinamensis (L.), Sitophilus oryzae (L.), Rhyzopertha dominica (F.) and Tribolium castaneum (Herbst), were 1.87, 2.35, 3.12 and 6.65 mg L-1, respectively. Penetration tests revealed that acrolein vapors could penetrate into the wheat mass and kill concealed insects in interk...

  2. Studies on the Toxicity of Acetone, Acrolein and Carbon Dioxide on Stored-Product Insects and Wheat Seed

    OpenAIRE

    Ali Asghar Pourmirza; Mehdie Tajbakhsh

    2008-01-01

    In laboratory experiments toxicity of acetone, acrolein and carbon dioxide were investigated against 4 species of stored-product insects. In all experiments, acrolein was the most toxic compound to the tested insects. In empty-space trials, estimated LD50 values of acrolein for adults of Tribolium castaneum (Herbst) (Tenebrionidae), Rhizopertha dominica (F.) (Bostrychidae), Sitophilus oryzae L. (Curculionidae) and Oryzaephilus surinamensis L. (Silvanidae) were 7.26, 6.09, 6.37 and 5.65...

  3. Chemical Composition and Bioactivities of the Essential Oil from Etlingera yunnanensis against Two Stored Product Insects

    OpenAIRE

    Shan-Shan Guo; Chun-Xue You; Jun-Yu Liang; Wen-Juan Zhang; Zhu-Feng Geng; Cheng-Fang Wang; Shu-Shan Du; Ning Lei

    2015-01-01

    The chemical composition of the essential oil of Etlingera yunnanensis rhizomes and its contact and repellent activities against Tribolium castaneum (Herbst) and Liposcelis bostrychophila (Badonnel) were investigated. The essential oil obtained from E. yunnanensis rhizomes with hydrodistillation was performed by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. The main components of the essential oil were identified to be estragole (65.2%), β-caryophylle...

  4. Observations préliminaires sur les fluctuations saisonnières des populations d'insectes ravageurs du mil stocké en grenier traditionnel au Sénégal

    OpenAIRE

    Seck, D.

    1991-01-01

    Preliminary notes about seasonal fluctuations of destructive insects of stored mil in traditional storehouses in Senegal. Insect flight populations infesting traditional millet storage have been followed by sticky trap during fifteen months in Senegal. Distribution analysis of species show that Sitotroga cerealella Oliv. (52 %) of insect total number is most abundant, following by Ephestia cautella WLK. (7V, 6 %), Tribolium castaneum Herbst. (17, 4 % o), Cryptolestes sp. (10, 1 % o) and Rhyzo...

  5. Leaves of Lantana camara Linn. (Verbenaceae) as a potential insecticide for the management of three species of stored grain insect pests

    OpenAIRE

    Rajashekar, Y.; Ravindra, K.V.; Bakthavatsalam, N.

    2012-01-01

    Insects cause extensive damage to stored grains and their value added products. Among the stored grain pests Sitophilus oryzae (L.) Callosobruchus chinensis (Fab.) and Tribolium castaneum (Herbst.) are considered as destructive pests in India. Plants may provide alternatives to currently used insect control agents as they constitute rich source in bioactive molecules. Lantana camara, an erect shrub, which grows widely in the tropics, exhibits insecticidal activity against several insects. The...

  6. Chemical Constituents and Toxicity of Essential Oils of Oriental Arborvitae, Platycladus orientalis (L.) Franco, against Three Stored-Product Beetles Componentes Químicos y Toxicidad de Aceites Esenciales de Tuya Oriental, Platycladus orientalis (L.) Franco, contra Tres Escarabajos de Productos Almacenados

    OpenAIRE

    Seyed Mehdi Hashemi; Seyed Ali Safavi

    2012-01-01

    Plant secondary metabolites play an important role in plant-insect interactions and therefore such compounds may have insecticidal or biological activity against insects. Fumigant toxicity of essential oils of leaves and fruits from oriental arborvitae (Platycladus orientalis (L.) Franco) (Cupressaceae) was investigated against adults of cowpea weevil (Callosobruchus maculatus Fab.), rice weevil (Sitophilus oryzae L.), and red flour beetle (Tribolium castaneum Herbst). Fresh leaves and fruits...

  7. Parasitylenchus bifurcatus n. sp. (Tylenchida: Allantonematidae parasitizing Harmonia axyridis (Coleoptera: Coccinellidae

    Directory of Open Access Journals (Sweden)

    Poinar George O

    2012-10-01

    Full Text Available Abstract Background The harlequin ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae is native to central and eastern Asia and was purposely introduced into Europe to control aphids. While it proved to be a good biological control agent, its rapid spread and buildup of large populations made it a nuisance, since it overwinters in homes, emits unpleasant odors, stains fabrics, occasionally bites humans and feeds on apples, pears and grapes. Aside from the above, the ravenous appetite of H. axyridis results in their consumption of harmless native insects, including even other ladybird beetles. A study of the natural enemies of H. axyridis in Denmark revealed the presence of nematodes. The present study describes this nematode parasite and discusses aspects of its development and ecology. Methods Adult harlequin ladybird beetles were collected from March to November from four localities in Copenhagen on different plant species. In addition, groups of last-instar larvae and pupae (n = 50 were examined for the presence of nematodes. Living and recently dead nematodes were removed from adult H. axyridis in 0.5% saline solution, the nematodes were then heat killed (at 75C, fixed in 5% formalin and transferred to glycerin on slides for further examination and measurements. Results A new species of Allantonematidae (Tylenchida, Parasitylenchus bifurcatus n. sp., is described from adults of the harlequin ladybird, Harmonia axyridis in Denmark. The new species is characterized by a straight stylet lacking basal thickenings, a bursa and a forked tail tip in the vermiform (infective females and juvenile males. The new species is compared with P. coccinellinae previously described from ladybird beetles in France. Parasitism resulted in depletion of the fat body and partial or complete atrophy of the reproductive organs of the beetles. Infections occurred throughout the year with rates of parasitism reaching up to 35%. The rate increased to 60

  8. Entomofauna Associada a Galhos de Acacia mangium Willd. Roletados por Oncideres saga (Dalman (Coleoptera: Cerambycidae

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    Gláucia Cordeiro

    2010-04-01

    Full Text Available O estudo da entomofauna associada aos galhos e fustes roletados por Oncideres saga (Dalman é importante para conhecermos seus possíveis inimigos naturais. Portanto, este trabalho teve como objetivo registrar os insetos associados aos galhos e fustes de Acacia mangium Willd. roletados por O. saga, em Coimbra-MG. Galhos e fustes roletados de A. mangium foram coletados de janeiro a abril de 2007. Este material foi vistoriado, armazenados em sacolas, feitas com tela plástica, e mantidos em sala com condições controladas (25,4±0,3°C e 66,7±1,4%. Constatou-se a presença de uma espécie, não determinada, de Scolytidae e a emergência de quatro espécies de Cerambycidae: Engyum quadrinotatum Thomsom; Eburodacrys sexmaculata (Olivier; Achryson surinamum (Linnaeus e Neoclytus pusillus (Laporte & Gory. Isto posto, fica evidenciada a necessidade de estudos com o objetivo de verificar qual o comportamento destes insetos em relação ao serrador O. saga.Insects associated with branches of Acacia mangium Willd. girdled by Oncideres saga (Dalman (Coleoptera: CerambycidaeAbstract. The study of the insects associated with branches and stems girdled by Oncideres saga (Dalman is important to know its possible natural enemies. Therefore, these work had the objective of register the insects associated with branches and stems girdled of Acacia mangium Willd. by this twig girdler beetle, in Coimbra, state of Minas Gerais, Brazil. Stems and branches of A. mangium were collected in January/2007 to April/2007. This material has been inspected, stored in plastic bags, and kept in a room with controlled conditions (25.4 ± 0.3°C and 66.7 ± 1.4%. It was noted the presence of a non-determined species of Scolytidae and the emergence of four species of Cerambycidae: Engyum quadrinotatum Thomsom; Eburodacrys sexmaculata (Olivier; Achryson surinamum (Linnaeus and Neoclytus pusillus (Laporte & Gory. It can be concluded that studies are needed with the objective of

  9. Karyotypes of some medium-sized Dytiscidae (Agabinae and Colymbetinae (Coleoptera

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    Robert Angus

    2013-06-01

    Full Text Available An account is given of the karyotypes of 29 species of medium sized Dytiscidae (Coleoptera. Of the 20 species of Agabus Leach, 1817, 18 have karyotypes comprising 21 pairs of autosomes and sex chromosomes which are either X0(♂ or XX (♀. These species are A. serricornis (Paykull, 1799, A. labiatus (Brahm, 1791, A. congener (Thunberg, 1794, A. lapponicus (Thomson, 1867, A. thomsoni (J. Sahlberg, 1871, A. confinis (Gyllenhal, 1808, A. sturmii (Gyllenhal, 1808, A. bipustulatus (Linnaeus, 1767, A. nevadensis Håkan Lindberg, 1939, A. wollastoni Sharp, 1882, A. melanarius Aubé, 1837, A. biguttatus (Olivier, 1795, A. binotatus Aubé, 1837, A. affinis (Paykull, 1798, A. unguicularis (Thomson, 1867, A. ramblae Millan & Ribera, 2001, A. conspersus (Marsham, 1802 and A. nebulosus (Forster, 1771. However two species, A. infuscatus Aubé, 1838 and A. adpressus Aubé, 1837, have developed a neo-XY system, with karyotypes comprising 21 pairs of autosomes and XY sex chromosomes (♂. No chromosomal differences have been detected between typical A. bipustulatus and A. bipustulatus var. solieri Aubé, 1837, nor have any been found between the three species of the A. bipustulatus complex (A. bipustulatus, A. nevadensis and A. wollastoni. The four species of Colymbetes Clairville, 1806, C. fuscus (Linnaeus, 1758, C. paykulli Erichson, 1837, C. piceus Klug, 1834 and C. striatus (Linnaeus, 1758 have karyotypes comprising 20 pairs of autosomes and sex chromosomes which are X0 (♂, XX (♀. Two of the species of Rhantus Dejean, 1833, R. exsoletus (Forster, 1771 and R. suturellus (Harris, 1828 have karyotypes comprising 20 pairs of autosomes and X0/XX sex chromosomes, but the other three species, R. grapii (Gyllenhal, 1808, R. frontalis (Marsham, 1802 and R. suturalis (Macleay, 1825 have 22 pairs of autosomes and X0/XX sex chromosomes. Agabus congener and Rhantus suturellus may have one B-chromosome. Nine of the species have previously published karyotype data but

  10. Susceptibility of Agrilus planipennis (Coleoptera: Buprestidae) to Beauveria bassiana and Metarhizium anisopliae.

    Science.gov (United States)

    Liu, Houping; Bauer, Leah S

    2006-08-01

    The susceptibility of Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) to selected strains of the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin was evaluated through bioassays with direct immersion or foliar exposure under laboratory conditions. Results showed that A. planipennis adults were susceptible to B. bassiana and M. anisoplae. Significant time-mortality response was found for each isolates. Isolate B. bassiana GHA killed A. planipennis adults at a faster rate compared with other isolates tested, with the lowest average time-to-death values. The LC50 values estimated under direct immersion method ranged from 1.7 x 10(5) to 1.9 x 10(7), 3.5 x 10(4) to 5.3 x 10(5), and 4.1 x 10(3) to 2.9 x 10(5) conidia/ml for B. basissiana and from 3.2 x 10(6) to 1.1 x 10(7), 4.5 x 10(3) to 4.5 x 10(5), and 1.4 x 10(2) to 1.2 x 10(5) conidia/ml for M. anisopliae at 4, 5, and 6 d after treatment, respectively. By days 5 and 6, B. bassiana GHA outperformed all other isolates tested except ARSEF 7234, followed by ARSEF 7152, 6393, and 7180. Significant concentration-mortality response was also observed for two B. bassiana GHA formulations, BotaniGard ES and Mycotrol O, and M. anisopliae F52 when insects were treated through foliar exposure. The LC50 values ranged from 114.5 to 309.6, 18.4 to 797.3, and 345.3 to 362.0 conidia/cm2 for BotaniGard, Mycotrol, and M. anisopliae F52, respectively. Based on the results of these bioassays, the efficacy of both B. bassiana GHA formulations and M. anisopliae F52 were similar against adult A. planipennis. The potential use of entomopathogenic fungi for management of A. planipennis in North America is discussed. PMID:16937660

  11. Diversity and altitudinal distribution of Chrysomelidae (Coleoptera in Peregrina Canyon, Tamaulipas, Mexico

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    Uriel Jeshua Sánchez-Reyes

    2014-06-01

    Full Text Available The Chrysomelidae (Coleoptera is a highly speciose family that has been poorly studied at the regional level in Mexico. In the present study, we estimated species richness and diversity in oak-pine forest, Tamaulipan thorny scrub and in tropical deciduous forests in Peregrina Canyon within the Altas Cumbres Protected Area of the northeastern state of Tamaulipas, Mexico. Sampling of Chrysomelidae consisted of five sweep net samples (200 net sweeps within each of three sites during four sample periods: early dry season, late dry season, early wet season, and late wet season. Species were identified and total numbers per species were recorded for each sample. A total of 2,226 specimens were collected belonging to six subfamilies, 81 genera and 157 species of Chrysomelidae from the study area. Galerucinae was the most abundant subfamily with 1,828 specimens, representing 82.1% of total abundance in the study area. Lower abundance was recorded in Cassidinae (8.5%, Eumolpinae (3.6%, Cryptocephalinae (2.2%, Chrysomelinae (2.2%, and finally Criocerinae (1.3%. The highest species richness was also presented in the subfamily Galerucinae with 49% of the total obtained species followed by Cassidinae (20%, Cryptocephalinae (9.7%, Eumolpinae (9.7%, Chrysomelinae (6.5% and Criocerinae (5.2%. The most common species were Centralaphthona fulvipennis Jacoby (412 individuals, Centralaphthona diversa (Baly (248, Margaridisa sp.1 (219, Acallepitrix sp.1 (134, Longitarsus sp.1 (104, Heterispa vinula (Erichson (91, Epitrix sp.1 (84 and Chaetocnema sp.1 (72. Twenty-two species were doubletons (1.97% of total abundance and 52 were singletons (2.33%. The estimated overall density value obtained was 0.0037 individuals/m2. The greatest abundance and density of individuals were recorded at the lowest elevation site. However, alpha diversity increased with increasing altitude. Similarity values were less than 50% among the three sites indicating that each site had distinct

  12. Oryctini (Coleoptera: Scarabaeidae: Dynastinae da coleção de invertebrados do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, Brasil Oryctini (Coleoptera: Scarabaeidae: Dynastinae of the invertebrate collection of the National Institute of Research of Amazonia (INPA, Manaus, Amazonas, Brazil

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    Hector Jaime Gasca

    2009-09-01

    Full Text Available A Coleção de Invertebrados do Instituto Nacional de Pesquisas da Amazônia (INPA possui 554 espécimes de Oryctini (Coleoptera: Scarabaeidae: Dynastinae, pertencentes a 7 gêneros, 16 espécies e 2 subespécies. As distribuições geográficas das espécies são fornecidas, sendo que 97% do material examinado procedem de coletas feitas em diferentes locais da Amazônia brasileira.The Invertebrate Collection of the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, Brazil, has 554 specimens of Oryctini (Coleoptera: Scarabaeidae: Dynastinae, belonging to seven genera, 16 species and two subspecies. Information about geographical distribution are provided, of which 97% of the material examined was collected from several places in the Brazilian Amazon.

  13. Occurrence and biology of Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitising different species of Coccinellidae (Coleoptera) in Neotropical region Ocorrência e biologia de Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitando diferentes espécies de Coccinellidae (Coleoptera) na região Neotropical

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    RB Silva; I Cruz; MLC Figueiredo; AG Pereira; AM Penteado-Dias

    2012-01-01

    Surveys on Coccinellidae (Coleoptera) in Sete Lagoas city, Minas Gerais state, Brazil, indicated the parasitism of adults of the species Coleomegilla maculata De Geer, 1775, Eriopis connexa (Germar, 1824) and Olla v-nigrum (Mulsant, 1866), by Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae). Since then, the parasitoid have been maintained in its original hosts at the Insect Rearing Laboratory - LACRI of the Brazilian Agricultural Research Institution - Embrapa Milh...

  14. Fauna de Coleoptera no Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brasil: abundância e riqueza das famílias capturadas através de armadilhas de solo Coleoptera fauna in the Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brazil: abundance and family richness captured with pitfall traps

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    Renato C. Marinoni; Norma G. Ganho

    2003-01-01

    This paper is a part of the studies on the Coleoptera fauna from Vila Velha, Ponta Grossa, Parana gathered through malaise and pitfall traps in sites with different floristic conditions. The present study deal with the data of pitfall trap captures, installed close to the malaise traps. The data were obtained weekly (52 samples), from September 1999 to August 2000. This survey was carried out on five areas, three of them with different plant succession stages (initial, intermediate, and advan...

  15. Biological and Morphological Aspects of Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), a Pest of Apulian Pinewoods in Southern Italy Aspetos da Biologia e Morfologia da Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), Praga nos Pinhais da Apúlia, Sul de Itália

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    Eustachio Tarasco; Oreste Triggiani

    2011-01-01

    Huge defoliations by Anoxia (Mesanoxia) matutinalis spp. matutinalis Castelnau, 1832 (Coleoptera, Scarabeidae) have been observed in recent years in the coastal pinewoods (Pinus halepensis, P. pinea) of the southern Apulia Region of Italy. Starting from adults collected in the field, insect breeding was conducted in the laboratory, following the life-cycle from ovideposition to larval development and emergence. Observations were performed in the laboratory and the field to study the different...

  16. Levantamento da fauna de Coleoptera que habita a carcaça de Sus scrofa L., em Curitiba, Paraná A study of the Coleoptera (Insecta fauna that inhabits Sus scrofa L. carcass in Curitiba, Paraná

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    Kleber Makoto Mise

    2007-09-01

    Full Text Available Este trabalho visou levantar a fauna de Coleoptera associada à carcaça de Sus scrofa L.,1758, espécie utilizada como modelo em Entomologia Forense. O acréscimo ou a substituição seqüencial das espécies de insetos pode ser utilizado para estimar o intervalo post mortem (IPM. O experimento foi realizado no Centro Politécnico (UFPR, de setembro de 2005 a setembro de 2006. A cada estação foi sacrificado um suíno de 15 kg no local, colocado em gaiola. A captura dos insetos foi realizada diariamente em bandeja posicionada abaixo da carcaça e em armadilha tipo Shannon modificada, e a cada 14 dias em cinco armadilhas do tipo pit-fall. Foram coletados 4.360 Coleoptera, pertencentes a 112 espécies de 26 famílias, 12 consideradas de importância forense. A coleta ativa realizada na bandeja foi responsável pela maior captura (2.023 espécimes, seguida pela armadilha Shannon modificada (2.016 espécimes e por último pelas do tipo pit-fall (324 espécimes. Staphylinidae foi mais coletada na bandeja e Shannon modificada, e Silphidae na armadilha pit-fall. Os principais hábitos encontrados foram predador/parasita (55%e onívoro (38,05%, com poucas espécies consideradas necrófagas (1,31%.This paper sought to assess the Coleoptera fauna associated with carcasses of Sus scrofa L., 1758, which is usually used as model in Forensic Entomology. The addition and sequential substitution of insect species could be used to estimate the post mortem interval (PMI. The present study took place in Centro Politécnico (UFPR, between september 2005 to september 2006. A pig weighting 15 kg was sacrificed each season and put inside a cage. Sampling is made daily in a tray placed below the carcass and in a Shannon modified trap, and each 14 days in five pit-fall traps. 4,360 beetles were collected, belonging to 112 species of 26 families, 12 were considered of forensic potential. The active collecting made in the tray was responsible for the largest number of

  17. Os besouros (Insecta: Coleoptera na concepção dos moradores de Pedra Branca, Santa Terezinha, Estado da Bahia = The term “beetle” (Insecta: Coleoptera as perceived and used by dwellers of Pedra Branca, Bahia, Brazil

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    Eraldo Medeiros Costa Neto

    2006-01-01

    Full Text Available Besouros (Insecta: Coleoptera são percebidos, conhecidos e utilizados por moradores do povoado de Pedra Branca, localizado no Estado da Bahia, Brasil. O trabalho de campo foi realizado entre abril e dezembro de 2004. Os dados foram obtidos com 49 indivíduos (20 do sexo masculino e 29 do sexo feminino por meio de entrevistas semiestruturadas e gravadas, seguindo-se uma abordagem emicista. Os resultados mostram que o genérico “besouro” é usado como uma categoria etnotaxonômica que reúne organismos sistematicamente não relacionados, além dos coleópteros da classificação lineana. Foramcitados 43 nomes locais de besouros. Os informantes atribuíram características qualitativas aos “besouros”, muitas das quais são antropomórficas. Algumas etnoespécies de “besouros” causam danos aos moradores de Pedra Branca, uma vez que ameaçam a saúde, os materiais,os cultivos locais e plantas frutíferas. Registrou-se informação sobre a história natural de “besouros” culturalmente importantes. Conclui-se que o conhecimento etnoentomológico dos moradores de Pedra Branca sobre besouros é importante como uma fonte de informação sobre as espécies locais.Fieldwork was carried out from April to December 2004. Data were obtained from 49 individuals (20 men and 29 women by means of open-ended tape-recorded interviews and following an emicist approach. Results show that the generic taxon “beetle” is used as an ethnotaxonomic category that brings together not systematically related organisms, besides the Coleoptera of the Linnaean classification. A total of 43 beetle local names were cited. People have attributed qualitative characteristics to these “beetles”, many of which are anthropomorphic. “Beetles” cause damages to human beings since they harm their health, materials, and local crops and fructiferous plants. Information on the natural history of culturally significant “beetles” was recorded. It is concluded that

  18. Notes on three braconid wasps (Hymenoptera: Braconidae, Doryctinae) parasitizing oak long-horned beetle, Massicus raddei (Coleoptera: Cerambycidae), a severe pest of Quercus spp. in China, together with the description of a new species.

    Science.gov (United States)

    Cao, Liangming; Yang, Zhongqi; Tang, Yanlong; Wang, Xiaoyi

    2015-01-01

    Three species of Doryctinae (Hymenoptera: Braconidae) parasitize larvae of oak longhorn beetle Massicus raddei Blessig (Coleoptera: Cerambycidae), a serious wood borer pest in North China. Rhoptrocentrus quercusi sp. nov., is described as a new species and Doryctes petiolatus Shestakov, as well as Zombrus bicolor (Enderlein). The three species are idiobiont ectoparasitoids, and may have potential for biological control of oak longhorn beetle. PMID:26624143

  19. Rozšíření rýhovců Rhysodes sulcatus (Fabricius, 1787) a Omoglymmius germari (Ganglbauer, 1892) (Coleoptera: Rhysodidae) v České republice

    Czech Academy of Sciences Publication Activity Database

    Konvička, Ondřej; Čížek, Lukáš

    2015-01-01

    Roč. 6, Sept 1 (2015), s. 111-114. ISSN 1804-2732 R&D Projects: GA ČR GAP504/12/1952 Institutional support: RVO:60077344 Keywords : Coleoptera * distributional maps * faunistics Subject RIV: EH - Ecology, Behaviour

  20. Efecto de la dieta artificial MP sobre la emergencia y relacion de sexos de Phymastichus coffea (Hymenoptera:Eulophidae) mantenido sobre su hueped, Hypothenemus hampei (Coleoptera: Scloytidae)a traves de generaciones contin

    Science.gov (United States)

    Phymastichus coffea La Salle (Hymenoptera: Eulophidae) is an endoparasitoid that attacks the adult coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Scolytidae). The MP diet developed by Portilla and Streett is the only reported diet that allows cultures of P. coffea to develop and repr...

  1. Field parasitism of nontarget weevil species (Coleoptera: Curculionidae) by the introduced biological control agent Microctonus aethiopoides Loan (Hymenoptera: Braconidae) over an altitude gradient.

    Science.gov (United States)

    Barratt, B I P; Ferguson, C M; Bixley, A S; Crook, K E; Barton, D M; Johnstone, P D

    2007-08-01

    The parasitoid, Microctonus aethiopoides Loan (Hymenoptera: Braconidae) was introduced into New Zealand in 1982 to control the alfalfa pest, Sitona discoideus Gyllenhal (Coleoptera: Curculionidae). Studies have shown that a number of nontarget weevil species are attacked in the field by this parasitoid. A field study was carried out to investigate nontarget parasitism by M. aethiopoides over an altitudinal sequence from the target host habitat (alfalfa) into native grassland. Three locations were selected for the study, and at each, the alfalfa growing in the valley floor was sampled annually for parasitism of the target pest that ranged between 17 and 78%. At progressively higher altitudes, three further grassland sites at each location were sampled monthly during spring to autumn for up to 6 yr. Weevil densities were estimated, species identified, and dissections carried out to determine reproductive status and parasitism. Almost 12,000 weevils were collected during the study, which were identified as 36 species in total from the three locations. Eight weevil species were found to be parasitized, including S. discoideus, the target host that was found at all sites. Parasitism of nontarget species was approximately 2% overall but varied with location, site, and season. Substantial nontarget parasitism was found at only one of the locations, with up to 24% parasitism of a native weevil, Nicaeana fraudator Broun (Coleoptera: Curculionidae), recorded. Another species, Irenimus egens (Broun) (Coleoptera: Curculionidae), was also found at this location at similar population densities but was attacked far less by M. aethiopoides. Results are discussed in relation to weevil phenology. PMID:17716473

  2. Aspectos biológicos e técnica de criação do gorgulho-da-goiaba, Conotrachelus psidii marshall (Coleoptera: Curculionidae)

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    Fabricio Iglesias Valente; Vera Lúcia Rodrigues Machado Benassi

    2014-01-01

    Considerada praga-chave da cultura da goiaba, Conotrachelus psidii Marshall (Coleoptera: Curculionidae) tem causado grandes prejuízos em vários países produtores desta fruta. A pesquisa objetivou avaliar os parâmetros biológicos da praga, sob condições controladas, através do desenvolvimento de uma técnica de criação que permitiu efetuar observações diárias de pré-pupas e pupas. Constatou-se que o substrato adequado para manutenção dessas fases foi o solo argiloso umedecido com viabilidade de...

  3. Description of the male of Eburella pinima Martins and notes on the geographical distribution of Eburodacrys aenigma Galileo & Martins and Eburodacrys lanei Zajciw (Coleoptera, Cerambycidae

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    Juan Pablo Botero

    2013-12-01

    Full Text Available Description of the male of Eburella pinima Martins and notes on the geographical distribution of Eburodacrys aenigma Galileo & Martins and Eburodacrys lanei Zajciw (Coleoptera, Cerambycidae. The male of Eburella pinima Martins, 1997 is described and illustrated for the first time. Information on Eburodacrys aenigma Galileo & Martins, 2006, previously known only from the female holotype, which lacked locality label, is herein complemented. This species is recorded from Brazil and the male is depicted for the first time. The geographical distribution of Eburodacrys lanei Zajciw, 1958 is further restricted here as some previous records are confirmed to result from misidentifications of E. aenigma.

  4. The infestation by an exotic ambrosia beetle, Euplatypus parallelus (F. (Coleoptera: Curculionidae: Platypodinae of Angsana trees (Pterocarpus indicus Willd. in southern Thailand

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    Sara Bumrungsri

    2008-07-01

    Full Text Available An exotic ambrosia beetle, Euplatypus parallelus (F. was collected from infested Pterocarpus indicus Willd. trees in Prince of Songkla University. Larvae and eggs were found in simple galleries with a single branch. Either a single male or a male and a female were found in each gallery. Half of these infested trees were previously attacked by long-horned beetles probably Aristobia horridula (Hope (Coleoptera: Cerambycidae, while some of them appeared to be healthy. Fusarium oxysporum Schlecht.:Fr. was isolated from frass, sapwood samples and insect larvae, and might be a cause of death of P.indicus.

  5. A new species of endogean, anophthalmous Parazuphium Jeannel from Northern Morocco (Coleoptera: Carabidae, with new molecular data for the tribe Zuphiini

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    Carmelo Andújar

    2011-06-01

    Full Text Available A new species of the genus Parazuphium (Coleoptera, Carabidae, Zuphiini, Parazuphium aguilerai sp.n., is described from the Tingitan peninsula in North Morocco. The single known specimen was found below a large stone deeply inserted in the substratum, and it is anophthalmous, depigmented and flattened. This is the second species of blind Parazuphium, the other being P. feloi Machado 1998 from a lave tube in the Canary Islands. Molecular data of the unique known P. aguilerai sp.n. specimen is provided, and a molecular phylogeny confirm its inclusion inside Zuphiini within Harpalinae. Identification keys to the Mediterranean and Macaronesian species of Parazuphium are provided.

  6. Estudio taxonómico de las especies de la familia bruchidae (Insecta: Coleoptera) relacionadas con el género Desmodium (Fabaceae) en el mundo.

    OpenAIRE

    Vásquez Chan, Adriano

    2011-01-01

    La presente investigación se realizó con el propósito de determinar las especies de brúquidos (Coleoptera: Bruchidae) que se alimentan de semillas de plantas del género Desmodium Desv. (Leguminosae: Papilionoideae) en el mundo. Se desarrollaron claves dicotómicas para determinar géneros y especies de brúquidos del Nuevo y Viejo Mundo, además de ampliar la información existente sobre la asociación brúquido-Desmodium de la base de datos BRUCOL. Se revisaron semillas de Desmodium infestadas por ...

  7. A new species of bromeliad-feeding Cephaloleia Chevrolat (Coleoptera, Chrysomelidae, Cassidinae from Costa Rica: evidence from DNA barcodes, larval and adult morphology and insect diets

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    Carlos Garcia-Robledo

    2015-01-01

    Full Text Available The Neotropical genus Cephaloleia Chevrolat (Coleoptera: Chrysomelidae: Cassidinae includes 214 species distributed from the south of Mexico to Argentina. Cephaloleia beetles feed mostly on plants from the order Zingiberales. The interactions between Cephaloleia beetles and their Zingiberales host plants is proposed as one of the oldest and most conservative associations. Here we describe a new species of Cephaloleia (C. kuprewiczae sp. n. that feeds on two species of bromeliads (Pitcairnia arcuata and P. brittoniana, Bromeliaceae: Pitcairnioideae. Cephaloleia kuprewiczae was previously described as Cephaloleia histrionica. This study includes evidence from DNA barcodes (COI, larval and adult morphology and insect diets that separates C. kuprewiczae from C. histrionica as a new species.

  8. Los ejemplares tipo de Dryopidae, Elmidae, Limnichidae y Psephenidae (Coleoptera: Byrrhoidea, depositados en la colección del Museo de La Plata The types of Dryopidae, Elmidae, Limnichidae and Psephenidae (Coleoptera: Byrrhoidea housed at the Museo de La Plata

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    Liliana A. Fernández

    2004-07-01

    Full Text Available Se examinaron los ejemplares tipo depositados en la colección de la División Entomología del Museo de La Plata, pertenecientes a diversas especies nominales de Byrrhoidea: Dryopidae (1 especie, 1 ejemplar tipo, Elmidae (4, 9, Limnichidae (29, 47 y Psephenidae (1, 2. El material tipo fue estudiado por los siguientes autores: Pic, Spangler, Spangler & Brown, Spangler & Perkins, Spangler & Santiago, Spilman y Wooldridge. Para cada taxón se indica la publicación original, datos de colección y condición del material.The types of species of Byrrhoidea (Coleoptera housed in the collection of the División Entomología of Museo de La Plata were examined and listed. They belong to 35 species distributed in the families Dryopidae (1 species, 1 type, Elmidae (4, 9, Limnichidae (29, 47 and Psephenidae (1, 2. The types were described by Pic, Spangler, Spangler & Brown, Spangler & Perkins, Spangler & Santiago, Spilman, and Wooldridge. For each taxon, complete information about categories of types, reference original descriptions, collection data and specimen condition are given.

  9. Occurrence of adults and biological aspects of Geniates borelli Camerano (Coleoptera, Scarabaeidae, Rutelinae in Aquidauana, MS, Brazil

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    Sergio Roberto Rodrigues

    2012-09-01

    Full Text Available Occurrence of adults and biological aspects of Geniates borelli Camerano (Coleoptera, Scarabaeidae, Rutelinae in Aquidauana, Mato Grosso do Sul, Brazil. Due to the importance of Geniates borelli Camerano as a pest in many crops, studies were developed at Universidade Estadual de Mato Grosso do Sul, Aquidauana campus, MS, Brazil. Adults were collected with a light trap from January 2006 to December 2007. 3,320 adults were collected, and the highest quantities were obtained in October 2006 and November 2007, with 1,548 and 802 adults recorded, respectively. Collected adults were kept in plastic containers with soil and Brachiaria decumbens seedlings for oviposition. 535 eggs measuring 2.30 × 1.60 mm were obtained. As the embryonic development progressed, eggs increased in size to 3.00 × 2.70 mm, and this change occurred between 6 and 10 days after oviposition. The embryonic period lasted 17.9 days. The first, second, and third instars lasted 37.6, 49.7, and 74 days, respectively. The prepupal stage lasted 65.9 days and the pupal stage lasted an average of 18.5 days. The biological cycle is completed in 315.8 days, which characterizes the species as univoltine. The average longevity of females was 35.4 days and 28.5 days for males.

  10. Molecular phylogeny of Diabrotica beetles (Coleoptera: Chrysomelidae) inferred from analysis of combined mitochondrial and nuclear DNA sequences.

    Science.gov (United States)

    Clark, T L; Meinke, L J; Foster, J E

    2001-08-01

    The phylogenetic relationships of thirteen Diabrotica (representing virgifera and fucata species groups) and two outgroup Acalymma beetle species (Coleoptera: Chrysomelidae) were inferred from the phylogenetic analysis of a combined data set of 1323 bp of mitochondrial DNA (mtDNA) cytochrome oxidase subunit 1 (COI) and the entire second internal transcribed spacer region (ITS-2) of nuclear ribosomal DNA of 362 characters. Species investigated were D. adelpha, D. balteata, D. barberi, D. cristata, D. lemniscata, D. longicornis, D. porracea, D. speciosa, D. undecimpunctata howardi, D. u. undecimpunctata, D. virgifera virgifera, D. v. zeae, D. viridula, and outgroup A. blandulum and A. vittatum. Maximum parsimony (MP), minimum evolution (ME), and maximum likelihood (ML) analyses of combined COI and ITS-2 sequences clearly place species into their traditional morphological species groups with MP and ME analyses resulting in identical topologies. Results generally confer with a prior work based on allozyme data, but within the virgifera species group, D. barberi and D. longicornis strongly resolve as sister taxa as well as monophyletic with the neotropical species, D. viridula, D. cristata and D. lemniscata also resolve as sister taxa. Both relationships are not in congruence with the prior allozyme-based hypothesis. Within the fucata species group, D. speciosa and D. balteata resolve as sister taxa. Results also strongly supported the D. virgifera and D. undecimpunctata subspecies complexes. Our proposed phylogeny provides some insight into current hypotheses regarding distribution status and evolution of various life history traits for Diabrotica. PMID:11520353

  11. Updated distribution of Osmoderma eremita in Abruzzo (Italy and agro-pastoral practices affecting its conservation (Coleoptera: Scarabaeidae

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    Patrizia Giangregorio

    2015-12-01

    Full Text Available New records of Osmoderma eremita (Scopoli, 1763 (Coleoptera: Scarabaeidae: Cetoniinae are reported for Abruzzo (Italy, together with a review of its distribution in this region. O. eremita is a saproxylic beetle dependent on the presence of hollow deciduous trees with abundant wood mould in their cavities. The major threats for the species are habitat loss and fragmentation. EU Habitats Directive requests to the member States its protection and the monitoring of its conservation status. Detection of its occurrence is the first step to protect the species. The surveys have been carried out in ten sites of Abruzzo by using black cross-windows traps baited with specific pheromone. The species has been recorded for the first time in the Sant’Antonio forest and its presence is confirmed in the Peligna Valley, after a decade. The populations seem to be confined to small patches of suitable habitats. At local level, the abandonment of the pollarding practice (willow and beech forests and the use of pollarded trees as biomass for fuel are the major threats for this species. Indeed some key actions, such as the protection of old hollow trees and the continuation of pollarding practice in rural landscape, could be key factors for the conservation strategies of the species in the study area.

  12. Population genetics and phylogenetic relationships of beetles (Coleoptera: Histeridae and Staphylinidae) from the Sonoran Desert associated with rotting columnar cacti.

    Science.gov (United States)

    Pfeiler, Edward; Johnson, Sarah; Richmond, Maxi Polihronakis; Markow, Therese A

    2013-12-01

    Dozens of arthropod species are known to feed and breed in the necrotic tissues (rots) of columnar cacti in the Sonoran Desert. Because the necrotic patches are ephemeral, the associated arthropods must continually disperse to new cacti and therefore the populations of any given species are expected to show very little local genetic differentiation. While this has been found to be true for the cactophilic Drosophila, the evolutionary histories and characteristics of other arthropods inhabiting the same necrotic patches, especially the beetles, have yet to be examined. Here we used nucleotide sequence data from segments of the mitochondrial 16S rRNA and cytochrome c oxidase subunit I (COI) genes to examine population structure and demographic history of three sympatric beetle species (Coleoptera: Histeridae and Staphylinidae) collected on senita cactus (Lophocereus schottii) from six widely-separated localities on the Baja California peninsula of northwestern Mexico. Two histerids, Iliotona beyeri and Carcinops gilensis, and an unidentified staphylinid, Belonuchus sp., showed little or no population structure over a broad geographic area on the peninsula, consistent with the prediction that these beetles should show high dispersal ability. Demographic tests revealed varying levels of historical population expansion among the beetle species analyzed, which are discussed in light of their ecologies and concurrent biogeographic events. Additionally, phylogenetic analyses of COI sequences in Carcinops collected on a variety of columnar cacti from both peninsular and mainland Mexico localities revealed several species-level partitions, including a putative undescribed peninsular species that occurred sympatrically with C. gilensis on senita. PMID:23948866

  13. Incidence of turf-damaging white grubs (Coleoptera: Scarabaeidae) and associated pathogens and parasitoids on Kentucky golf courses.

    Science.gov (United States)

    Redmond, Carl T; Potter, Daniel A

    2010-12-01

    Root-feeding grubs (Coleoptera: Scarabaeidae) were sampled from damaged areas of 61 irrigated roughs on 32 Kentucky golf courses to determine species composition and natural enemy incidence, the first such survey in the United States' transitional turfgrass climatic zone. Masked chafers (Cyclocephala lurida Bland and C. borealis Arrow) and Japanese beetle (Popillia japonica Newman) accounted for ≈73 and 26% of grubs found in an autumn survey, with Cyclocephala spp. predominating at most sites, although mixed infestations were common. Only a few Phyllophaga spp., and no exotic species other than P. japonica were found. Cyclocephala spp. also predominated in seasonal and statewide surveys regardless of whether a course had cool- or warm-season grass fairways. Pathogenic bacteria, Paenibacillus and Serratia spp., and the autumn-active parasitoid Tiphia pygidialis Allen were the main enemies associated with Cyclocephala spp. Predominant enemies of P. japonica were Paenibacillus, Serratia, and Metarhizium spp. in autumn, and eugregarines, Stictospora sp. (probably S. villani Hays and Clopton) and Tiphia vernalis Rohwer in spring. Entomopathogenic nematodes and the microsporidian Ovavesicula popilliae Andreadis & Hanula were nearly absent in our samples. No predictive relationships were found between soil parameters and proportionate abundance of Cyclocephala or P. japonica, or with natural enemy incidence at particular sites. Although incidence of individual enemies was generally low (hosts' prolonged development they may take a significant toll on grub populations. PMID:22182549

  14. Attraction of Tomicus yunnanensis (Coleoptera: Scolytidae to Yunnan Pine Logs with and without Periderm or Phloem: An Effective Monitoring Bait

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    Rong Chun Lu

    2012-01-01

    Full Text Available The Yunnan pine shoot beetle, Tomicus yunnanensis Kirkendall and Faccoli (Coleoptera: Scolytinae is an important pest of Yunnan pine (Pinus yunnanensis Franch in China. Experiments with host log baits were done to develop a pest monitoring system using host tree kairomone. Five Yunnan pine logs (each 10–15 cm diam. × 30-cm long in a trap-log bundle were treated by peeling periderm (outer bark off to expose the phloem, and half of each log was covered with sticky adhesive to capture any attracted adult beetles. Significantly, more beetles were attracted and caught on the periderm-peeled logs (ca 30 beetles/m2 log surface/day than on untreated control logs with adhesive (ca 2.5/m2/day. No significant differences were observed between catches on logs taken from lower or upper halves of Yunnan pines. T. yunnanensis flies mostly during the afternoon according to trap catches throughout the day. Attraction to the periderm-peeled logs decreased considerably when they were peeled further to remove the phloem, indicating phloem volatiles play a role in selection of the host by the beetle. The readily-available log baits appear useful for monitoring pine shoot beetle populations in integrated pest management programs.

  15. Morfología e histología del sistema digestivo del burrito del frambueso Aegorhinus superciliosus (Coleoptera: Curculionidae

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    VIVIAN MEDEL

    2013-12-01

    Full Text Available Se realizó el estudio morfológico e histológico del sistema digestivo de Aegorhinus superciliosus (Coleoptera: Curculionidae, plaga de importancia económica en el sur de Chile. Esta especie presenta un sistema digestivo característico para la mayoría de los insectos, siendo similar en machos y hembras, pero de mayor longitud en las últimas, llegando a medir el doble del tamaño del insecto. El sistema digestivo es un tubo largo de diámetro variable, dividido en tres regiones: intestino anterior (estomodeo, intestino medio (mesenterón e intestino posterior (proctodeo. Histológicamente presenta un tejido epitelial similar al descrito para otras especies y externamente un tejido muscular, sin diferencias entre machos y hembras. No obstante, presenta características que difieren en otros curculiónidos y coleópteros tales como: ausencia de buche, ubicación y número de los ciegos gástricos, presencia de membrana peritrófica y ausencia de almohadillas rectales.

  16. Occurrence and biology of Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitising different species of Coccinellidae (Coleoptera) in Neotropical region.

    Science.gov (United States)

    Silva, R B; Cruz, I; Figueiredo, M L C; Pereira, A G; Penteado-Dias, A M

    2012-02-01

    Surveys on Coccinellidae (Coleoptera) in Sete Lagoas city, Minas Gerais state, Brazil, indicated the parasitism of adults of the species Coleomegilla maculata De Geer, 1775, Eriopis connexa (Germar, 1824) and Olla v-nigrum (Mulsant, 1866), by Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae). Since then, the parasitoid have been maintained in its original hosts at the Insect Rearing Laboratory - LACRI of the Brazilian Agricultural Research Institution - Embrapa Milho e Sorgo. Besides the citation of occurrence in Brazil, this work also indicates the parasitoid preference for C. maculata (70% of parasitism), followed by O. v-nigrum (43.3% of parasitism) and E. connexa (36.7% of parasitism). Total life cycle of D. coccinellae was longer on C. maculata (32.4 ± 0.48 days), compared to O. v-nigrum (29.5 ± 0.49 days) and E. connexa (27.8 ± 0.4 days). Due to the relatively high percentage of field parasitism, D. coccinellae can reduce the efficiency of biological pest control by Coccinellidae predators especially in the case of C. maculata. PMID:22437405

  17. Coleoptera families other than Cerambycidae, Curculionidae sensu lato, Chrysomelidae sensu lato and Coccinelidae. Chapter 8.5

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    Olivier Denux

    2010-07-01

    Full Text Available Here we consider 274 alien Coleoptera species belonging to 41 of the 137 beetle families in Europe (Cerambycidae, Curculionidae sensu lato, Chrysomelidae sensu lato and Coccinelidae are treated separately elsewhere. Among the families we consider asinvaded the European fauna, Acanthocnemidae and Ptilodactylidae represent new arrivals. Many species-rich families have surprisingly few aliens, whereas some relatively minor families such as Dermestidae, Nitidulidae and Anobiidae have a relatively high representation of alien species. Since the start of the 19th century, the number of coleopteran aliens introduced into Europe has continued to increase. Alien species colonizing Europe derive from a wide range of geographic regions as well as ecozones, but the most important source area is Asia. The countries with the largest number of alien species established are France, Germany and Italy. The majority have been introduced accidentally via international transport mechanisms. The most important route for importation is stored products and crops, followed by transport of wood, then horticultural and ornamental plants. Most alien species in these families are found within anthropogenic habitats in Europe. The introduction of invasive alien beetles in these families has had significant economic impacts, particularly as pests of stored foodstuffs, as well as serious ecological impacts. For example, the buprestid species Agrilus planipennis, recently recorded in Russia, is an important potential economic threat which may also impact the biodiversity associated with ash trees.

  18. Inheritance and Fitness Costs of Resistance to Cry3Bb1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Ingber, David A; Gassmann, Aaron J

    2015-10-01

    Transgenic crops that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely planted to manage pest insects. One of the primary pests targeted by Bt corn in the United States is western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae). Cry3Bb1 corn for management of western corn rootworm was commercialized in 2003, and beginning in 2009, populations of western corn rootworm with field-evolved resistance to Cry3Bb1 corn were found in Iowa. Here we quantify the magnitude, inheritance, and fitness costs of resistance to Cry3Bb1 corn in two strains (Hopkinton and Cresco) derived from field populations that evolved resistance to Cry3Bb1 corn. For Hopkinton, we found evidence for complete resistance to Cry3Bb1 corn and nonrecessive inheritance. Additionally, no fitness costs of Cry3Bb1 resistance were detected for Hopkinton. For Cresco, resistance was incomplete and recessive, and we detected fitness costs affecting developmental rate, survival to adulthood, and fecundity. These results suggest that variation may exist among field populations in both the inheritance and accompanying fitness costs of resistance. To the extent that field populations exhibit nonrecessive inheritance and a lack of fitness cost, this will favor more rapid evolution of resistance than would be expected when resistance is functionally recessive and is accompanied by fitness costs. PMID:26453731

  19. A DNA barcode library of the beetle reference collection (Insecta: Coleoptera in the National Science Museum, Korea

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    Sang Woo Jung

    2016-06-01

    Full Text Available Coleoptera is a group of insects that are most diverse among insect resources. Although used as indicator species and applied in developing new drugs, it is difficult to identify them quickly. Since the development of a method using mitochondrial DNA information for identification, studies have been conducted in Korea to swiftly and accurately identify species. The National Science Museum of Korea (NSMK has been collecting and morphologically identifying domestic reference insects since 2013, and building a database of DNA barcodes with digital images. The NSMK completed construction of a database of digital images and DNA barcodes of 60 beetle species in the Korean National Research Information System. A total of 179 specimens and 60 species were used for the analysis, and the averages of intraspecific and interspecific variations were 0.70±0.45% and 26.34±6.01%, respectively, with variation rates ranging from 0% to 1.45% and 9.83% to 56.23%, respectively.

  20. Revision of the Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera, Dytiscidae, Hydroporinae, Bidessini)

    Science.gov (United States)

    Miller, Kelly B.

    2016-01-01

    Abstract The Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera: Dytiscidae: Hydroporinae: Bidessini) is revised. Thirty species are recognized. The following new species are described: Hydrodessus bimaculatus sp. n. (Venezuela), Hydrodessus brevis sp. n. (Venezuela), Hydrodessus concolorans sp. n. (Venezuela), Hydrodessus continuus sp. n. (Venezuela), Hydrodessus disjunctus sp. n. (Suriname), Hydrodessus fasciatus sp. n. (Brazil), Hydrodessus imparilis sp. n. (Ecuador), Hydrodessus keithi sp. n. (Brazil, Colombia, Ecuador), Hydrodessus kurti sp. n. (Suriname), Hydrodessus kylei sp. n. (Suriname, Venezuela), Hydrodessus laetus sp. n. (Venezuela), Hydrodessus latotibialis sp. n. (Peru), Hydrodessus maculatus sp. n. (Guyana, Venezuela), Hydrodessus morsus sp. n. (Venezuela), Hydrodessus palus sp. n. (Venezuela), and Hydrodessus tenuatus sp. n. (Suriname). The following new synonyms are established: Hydrodessus fragrans Spangler, 1985 = Hydrodessus biguttatus (Guignot, 1957) syn. n. and Hydrodessus robinae Spangler, 1985 = Hydrodessus octospilus (Guignot, 1957), syn. n. One species is transferred from Hydrodessus to Amarodytes Régimbart, Amarodytes soekhnandanae (Makhan, 1994), comb. n. Habitus photographs (dorsal and lateral) and photos of the ventral surfaces are provided for most species. Line drawings of male and female genitalia and other diagnostic features are also provided along with distribution maps.

  1. Effects of irradiated diet on longevity and prolificity of successive generations of Zabrotes subfasciatus (Boheman, 1833) (Coleoptera, Bruchidae)

    International Nuclear Information System (INIS)

    The study was done in the laboratory of Entomology of the Centro de Energia Nuclear na Agricultura (CENA), University of Sao Paulo, in Piracicaba, State of Sao Paulo, Brazil. This research was carried out to observe the effect of irradiated beans (Phaseolus vulgaris, (L.), cultivar Rosinha) on the longevity and prolificity of Zabrotes subfasciatus (Boheman, 1833) (Coleoptera, Bruchidae). The first part of the study included the rearing of five generations of Zabrotes subfasciatus which were fed with irradiated beans (0, 10, 20, 100 e 200 krad, with a dose rate of approximately 70 krad/h). The second one consisted of growth of seven generations on irradiated beans (0, 100, 500, 1500 and 3000 krad, with a dose rate of approximately 70 krad/h). The data were obtained through daily countings of insect birth and death rates. It was concluded that irradiation modified the nutritional quality of beans, affecting the development of Zabrotes subfasciatus. This effect was studied through the longevity and prolificity, which were altered as compared with the control, once they were higher for the dosis of 1500 and 3000 krad. (author)

  2. Effects of seed type and bruchid genotype on the performance and oviposition behavior of Zabrotes subfasciatus (Coleoptera: Bruchidae)

    Institute of Scientific and Technical Information of China (English)

    ERICK D. M. CAMPAN; BETTY BENREY

    2006-01-01

    The effect of different bean varieties on the performance of the bruchid beetle Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae), was determined by using wild and cultivated seeds of the genus Phaseolus. Results showed that the quality of the host plant affected the performance and the oviposition behavior of female beetles. Overall, bruchid performance was higher on cultivated seeds than on wild seeds. It was also found that the oviposition behavior and the performance of their offspring differed between females that originated from wild versus cultivated seeds. We also demonstrated the importance of a genetic component in bruchid performance: longevity, fecundity, larval development, adult For example, on the same host type, some females laid twice as many eggs as females from Thus, the performance and behavior of Z. subfasciatus are not only affected by environmental factors such as the quality of the seeds on which they develop, but also have a genetic basis which can counterbalance a less suitable quality of the host plant. For a crop pest such as Z.subfasciatus, its ability to survive and adapt on host plants of differing quality may be an important attribute to consider for pest management.

  3. Knockdown, residual, and antifeedant activity of pyrethroids and home landscape bioinsecticides against Japanese beetles (Coleoptera: Scarabaeidae) on Linden foliage.

    Science.gov (United States)

    Baumler, Rebecca E; Potter, Daniel A

    2007-04-01

    Residual toxicity and leaf protection capability of five pyrethroids, professional and home garden azadirachtin formulations, and six other bioinsecticides for the home landscape were evaluated against the Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), on linden, Tilia cordata L. Capacity of intoxicated beetles to recover and subsequently feed and disperse also was evaluated to provide insight on activity characteristics of the different compounds. Intact shoots were sprayed and left in the field for varying intervals before being challenged with beetles in no-choice and choice tests. All pyrethroids except permethrin gave greater leaf protection, knockdown, and kill than did carbaryl, the standard, after 14 d of weathering. Deltamethrin, cyfluthrin, bifenthrin, and lamda-cyhalothrin gave a high level of protection for at least 19 d, and azadirachtin (Azatin XL) deterred feeding in choice tests for as long as 14 d. Home garden formulations containing pyrethrins in canola oil (Pyola) or azadiractin (Neem-Away) gave good short-term (< 3-d) protection. Formulations of capsaicin, rotenone + pyrethrins, kaolin particle film, D-limonene, or garlic extract were ineffective, the latter two formulations being highly phytotoxic to linden. Results of this study should help support updating of guidelines for insecticidal control of Japanese beetles. PMID:17461070

  4. Biology and host preferences of Cryptorhynchus melastomae (Coleoptera: Curculionidae), a possible biocontrol agent for Miconia calvescens (Melastomataceae) in Hawaii.

    Science.gov (United States)

    Reichert, Elisabeth; Johnson, M Tracy; Chacón, Eduardo; Anderson, Robert S; Wheeler, Terry A

    2010-12-01

    The introduced plant Miconia calvescens (Melastomataceae) poses a grave threat to Hawaii's native ecosystems and biodiversity. One potential candidate for classical biological control is Cryptorhynchus melastomae (Coleoptera: Curculionidae: Cryptorhynchinae), a stem-boring weevil from Central and South America. This weevil feeds on M. calvescens in its native Costa Rica and has been successfully reared under greenhouse conditions. Comparison of its environmental conditions in Costa Rica with those in the Miconia infested areas of Hawaii indicates the latter is a suitable habitat for C. melastomae. C. melastomae has one or two generations per year. Adults feed on new stems, petioles, leaf buds, veins, and lamina, whereas larvae mine the stem until pupation. Adults appear to prefer saplings for oviposition and feeding. Under greenhouse conditions both adults and larvae can seriously damage and kill small M. calvescens. Preliminary host testing indicates that C. melastomae may be family specific on Melastomataceae. However, because Hawaii lacks native melastomes and has many other serious melastome weeds, a family specific insect may be suitable as a biocontrol agent in this case. PMID:22182550

  5. Acute toxicity of plant essential oils to scarab larvae (Coleoptera: Scarabaeidae) and their analysis by gas chromatography-mass spectrometry.

    Science.gov (United States)

    Ranger, Christopher M; Reding, Michael E; Oliver, Jason B; Moyseenko, James J; Youssef, Nadeer; Krause, Charles R

    2013-02-01

    Larvae of scarab beetles (Coleoptera: Scarabaeidae) are important contaminant and root-herbivore pests of ornamental crops. To develop alternatives to conventional insecticides, 24 plant-based essential oils were tested for their acute toxicity against third instars of the Japanese beetle Popillia japonica Newman, European chafer Rhizotrogus majalis (Razoumowsky), oriental beetle Anomala orientalis (Waterhouse), and northern masked chafer Cyclocephala borealis Arrow. Diluted solutions were topically applied to the thorax, which allowed for calculating LD50 and LD90 values associated with 1 d after treatment. A wide range in acute toxicity was observed across all four scarab species. Of the 24 oils tested, allyl isothiocyanate, cinnamon leaf, clove, garlic, and red thyme oils exhibited toxicity to all four species. Allyl isothiocyanate was the most toxic oil tested against the European chafer, and among the most toxic against the Japanese beetle, oriental beetle, and northern masked chafer. Red thyme was also comparatively toxic to the Japanese beetle, oriental beetle, European chafer, and northern masked chafer. Interspecific variability in susceptibility to the essential oils was documented, with 12, 11, 8, and 6 of the 24 essential oils being toxic to the oriental beetle, Japanese beetle, European chafer, and northern masked chafer, respectively. Analysis of the active oils by gas chromatography-mass spectrometry revealed a diverse array of compounds, mostly consisting of mono- and sesquiterpenes. These results will aid in identifying active oils and their constituents for optimizing the development of plant essential oil mixtures for use against scarab larvae. PMID:23448028

  6. Revision of the Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera, Dytiscidae, Hydroporinae, Bidessini).

    Science.gov (United States)

    Miller, Kelly B

    2016-01-01

    The Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera: Dytiscidae: Hydroporinae: Bidessini) is revised. Thirty species are recognized. The following new species are described: Hydrodessus bimaculatus sp. n. (Venezuela), Hydrodessus brevis sp. n. (Venezuela), Hydrodessus concolorans sp. n. (Venezuela), Hydrodessus continuus sp. n. (Venezuela), Hydrodessus disjunctus sp. n. (Suriname), Hydrodessus fasciatus sp. n. (Brazil), Hydrodessus imparilis sp. n. (Ecuador), Hydrodessus keithi sp. n. (Brazil, Colombia, Ecuador), Hydrodessus kurti sp. n. (Suriname), Hydrodessus kylei sp. n. (Suriname, Venezuela), Hydrodessus laetus sp. n. (Venezuela), Hydrodessus latotibialis sp. n. (Peru), Hydrodessus maculatus sp. n. (Guyana, Venezuela), Hydrodessus morsus sp. n. (Venezuela), Hydrodessus palus sp. n. (Venezuela), and Hydrodessus tenuatus sp. n. (Suriname). The following new synonyms are established: Hydrodessus fragrans Spangler, 1985 = Hydrodessus biguttatus (Guignot, 1957) syn. n. and Hydrodessus robinae Spangler, 1985 = Hydrodessus octospilus (Guignot, 1957), syn. n. One species is transferred from Hydrodessus to Amarodytes Régimbart, Amarodytes soekhnandanae (Makhan, 1994), comb. n. Habitus photographs (dorsal and lateral) and photos of the ventral surfaces are provided for most species. Line drawings of male and female genitalia and other diagnostic features are also provided along with distribution maps. PMID:27110208

  7. Developmental Biology and Effects of Adult Diet on Consumption, Longevity, and Fecundity of Colaspis crinicornis (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Miwa, Kentaro; Meinke, Lance J

    2015-01-01

    The chrysomelid beetle Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) occurs primarily in the Great Plains region of the United States. Little is known about the biology and ecology of this species, but over the last decade, it has become increasingly common in the corn, Zea mays L., and soybean, Glycine max (L.) Merrill, agroecosystem of southeastern Nebraska. As part of a larger comprehensive project to understand the natural history and pest potential of this species, laboratory experiments were conducted to study the developmental biology, morphological characters of immature stages, and the effect of adult diet on consumption, longevity, and fecundity. Females readily deposited egg clusters in the soil, and percentage egg hatch was high under laboratory conditions. Larvae and pupae were confirmed to be soil-dwelling stages. C. crinicornis has relatively short egg, pupal, and adult stages with the majority of its life cycle spent in the larval stage. Results of choice and no-choice adult feeding experiments indicate that diets of corn or soybean leaves did not significantly affect consumption, longevity, or fecundity of adult C. crinicornis, suggesting that corn and soybean leaves are similarly suitable food sources for adults. The ability to effectively utilize tissues from very different plant families as adult food sources suggests that C. crinicornis is polyphagous in the field. PMID:26106090

  8. Repellent Activity of the Essential Oil from the Heartwood of Pilgerodendron uviferum (D. Don) Florin against Aegorhinus superciliosus (Coleoptera: Curculionidae).

    Science.gov (United States)

    Espinoza, Javier; Urzúa, Alejandro; Tampe, Jocelyne; Parra, Leonardo; Quiroz, Andrés

    2016-01-01

    The weevil Aegorhinus superciliosus Guérin (Coleoptera: Curculionidae), which is endemic to Central-Southern Chile and Argentina, is one of the major berry pests in Chile and the most important pest in the La Araucanía Region (38°44'9″S, 72°35'25″W). Due to the poor effectiveness and problems surrounding the implementation of the traditional control methods using organophosphate and carbamate insecticides, new strategies for controlling this pest are needed. In this communication, we evaluated the behavioral responses of male and female A. superciliosus to volatile compounds released from the essential oil (EO) obtained from the heartwood of Pilgerodendron uviferum (D. Don) Florin using olfactometric bioassays. The composition of the EO was analyzed using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). According to these analyses, δ-cadinol (24.16%), cubenol (22.64%), 15-copaenol (15.46%) and δ-cadinene (10.81%) were the principal components of the EO. The Pilgerodendron uviferum EO, which is almost exclusively composed of sesquiterpenes (99.5%), exhibited a repellent effect against A. superciliosus adults, regardless of the sex or concentration used (56.6 mg/cm³ and 1.58 × 10(-2) mg/cm³). The EO has low volatility and greater persistence than the EOs composed of monoterpenes and is considered a good model in the search for raspberry weevil repellents. PMID:27110756

  9. Calliini (Coleoptera, Cerambycidae, Lamiinae: descrições, homonímia, novo registro e chave para as espécies de Graminea Thomson, 1864

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    Ubirajara R. Martins

    2006-01-01

    Full Text Available Calliini (Coleoptera, Cerambycidae, Lamiinae: descrições, homonímia, novo registro e chave para as espécies de Graminea Thomson, 1864. Espécies novas descritas: Paracallia giesberti sp. nov. da Bolívia (Cochabamba, Colombicallia albofasciata sp. nov. e Graminea rubra sp. nov. da Bolívia (Santa Cruz; Asemolea flava sp. nov. da Costa Rica, (Heredia; Callia potiaiuba sp. nov. do Brasil (Rondônia e da Bolívia (Cochabamba e Santa Cruz. É proposto o nome Rumuara nom. nov. para Paracladus Martins & Galileo, 1990 non Carpenter, 1966 (Orthoptera. Apresenta-se novo registro para a Bolívia para Eumimesis trilineata Magno & Monné, 1990. Acrescenta-se chave para as espécies de Graminea.Calliini (Coleoptera, Cerambycidae, Lamiinae: descriptions, homonym, new record and key to the species of Graminea Thomson,1864. New species described: Paracallia giesberti sp. nov. from Bolivia (Cochabamba, Colombicallia albofasciata sp. nov. and Graminea rubra sp. nov. from Bolivia (Santa Cruz; Asemolea flava sp. nov. from Costa Rica, (Heredia; Callia potiaiuba sp. nov. from Brazil (Rondônia and Bolivia (Cochabamba and Santa Cruz. Rumuara nom. nov. is proposed for Paracladus Martins & Galileo, 1990 non Carpenter, 1966 (Orthoptera. Eumimesis trilineata Magno & Monné, 1990 is recorded for Bolivia. A key to the species of Graminea is added.

  10. Molecular cloning and functional characterization of the sex-determination gene doublesex in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Coleoptera, Tenebrionidae)

    Science.gov (United States)

    Gotoh, Hiroki; Ishiguro, Mai; Nishikawa, Hideto; Morita, Shinichi; Okada, Kensuke; Miyatake, Takahisa; Yaginuma, Toshinobu; Niimi, Teruyuki

    2016-01-01

    Various types of weapon traits found in insect order Coleoptera are known as outstanding examples of sexually selected exaggerated characters. It is known that the sex determination gene doublesex (dsx) plays a significant role in sex-specific expression of weapon traits in various beetles belonging to the superfamily Scarabaeoidea. Although sex-specific weapon traits have evolved independently in various Coleopteran groups, developmental mechanisms of sex-specific expression have not been studied outside of the Scarabaeoidea. In order to test the hypothesis that dsx-dependent sex-specific expression of weapon traits is a general mechanism among the Coleoptera, we have characterized the dsx in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Tenebrionidea, Tenebirionidae). By using molecular cloning, we identified five splicing variants of Gnatocerus cornutus dsx (Gcdsx), which are predicted to code four different isoforms. We found one male-specific variant (GcDsx-M), two female-specific variants (GcDsx-FL and GcDsx-FS) and two non-sex-specific variants (correspond to a single isoform, GcDsx-C). Knockdown of all Dsx isoforms resulted in intersex phenotype both in male and female. Also, knockdown of all female-specific isoforms transformed females to intersex phenotype, while did not affect male phenotype. Our results clearly illustrate the important function of Gcdsx in determining sex-specific trait expression in both sexes. PMID:27404087

  11. Notas e descrições em Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. Novos táxons, nova sinonímia e novos registros

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    Ubirajara R. Martins

    2007-01-01

    Full Text Available Notas e descrições em Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. Novos táxons e novos registros. Descrição de espécies novas: Zikanita egregia sp. nov. (Bolívia, Santa Cruz; Dufauxia thomasi sp. nov. (Bolívia, Santa Cruz; Irundisaua punctata sp. nov. (Colômbia, Cauca; Cotyzineus gen. nov., espécie-tipo C. bruchi (Melzer, 1931 comb. nov. Novos registros: Zikanita argenteofasciata (Tippmann, 1960 é assinalada para o Brasil (Maranhão e Cotyzineus bruchi para a Bolívia (Santa Cruz. Novo sinônimo: Ateralphus diringsi Martins & Monné, 1993 = A. javariensis Lane, 1965.Notes and descriptions on Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. New taxa and new records. New taxa described: Zikanita egregia, sp. nov. from Bolivia (Santa Cruz; Dufauxia thomasi sp. nov. from Bolivia (Santa Cruz; Irundisaua punctata sp. nov. from Colombia (Cauca; Cotyzineus gen. nov. type species C. bruchi (Melzer, 1931 comb. nov. New records: Zikanita argenteofasciata (Tippmann, 1960 for Brazil (Maranhão; Cotyzineus bruchi for Bolivia (Santa Cruz. New synomym: Ateralphus diringsi Martins & Monné, 1993 = A. javariensis Lane, 1965.

  12. Egg Predation by the Introduced Lady Beetle, Coccinella septempunctata (Coleoptera: Coccinellidae, Lowers Mortality but Raises Relative Risk for the Native Lady Beetle, Coccinella novemnotata.

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    Rakim Turnipseed

    Full Text Available Populations of the native ninespotted lady beetle, Coccinella novemnotata Herbst, have undergone precipitous declines in North America following the establishment of the exotic sevenspotted lady beetle, Coccinella septempunctata L. Recent volunteer efforts have made it possible to establish colonies of the now-rare C. novemnotata and test mechanisms contributing to its decline. We evaluated the relative frequencies of intraguild predation and cannibalism of eggs between these two species. A single C. novemnotata or C. septempunctata adult was exposed to conspecific and heterospecific eggs in either the presence or absence of pea aphids. The study revealed two expected results: 1 eggs of C. novemnotata were consumed more frequently than eggs of C. septempunctata by both species, and 2 egg consumption was higher when aphids were absent, independent of predator and egg species. There were also two unexpected results from the study: 1 the asymmetry between egg predation rates was higher when aphids were present, and 2 higher predation rates on C. novemnotata eggs in the absence of alternate prey was due to a relatively higher rate of intraspecific cannibalism. This implies that C. novemnotata would have suffered higher egg mortality rates before the invasion of C. septempunctata, but even though the aggregate rate of egg predation on C. novemnotata eggs is lower post-invasion, it is still significantly higher than the aggregate rate of predation of C. septempunctata eggs. This differential pattern of asymmetric predation could contribute to habitat compression and the overall decline of C. novemnotata.

  13. Aspectos biológicos de Coccidophilus citricola (Coleoptera, Coccinellidae sobre Aspidiotus nerii e Chrysomphalus aonidum (Homoptera, Diaspididae Biological studies of Coccidophilus citricola (Coleoptera, Coccinellidae on Aspidiotus nerii and Chrysomphalus aonidum (Homoptera, Diaspididae

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    Antonio Cesar dos Santos

    2005-02-01

    Full Text Available Aspectos biológicos de Coccidophilus citricola Brèthes (Coleoptera, Coccinellidae foram estudados sobre Chrysomphalus aonidum Linnaeus e Aspidiotus nerii Bouchè (Homoptera, Diaspididae criadas sobre abóbora. Observou-se a duraç��o (dias do período de ovo, dos quatro ínstares, pré-pupa, pupa, longevidade de adultos e a proporção machos: fêmeas de C. citricola. Determinou-se também verificar a eficiência de predação de adultos de C. citricola sobre A. nerii. C. aonidum e A. nerii são presas adequadas para C. citricola, com desenvolvimento semelhante do coccinelídeo sobre essas cochonilhas; o período de incubação do ovo foi de 7,9 e 8,1 dias com A. nerii e C. aonidum; a duração (dias de cada ínstar larval de C. citricola sobre A. nerii e C. aonidum foram respectivamente de: (I 2,7 e 2,9; (II 3,1 e 3,3; (III 3,0 e 3,3 e (IV 3,2 e 3,1 dias. A duração das fases de pré-pupa e pupa foi de 2,5 e 4,5 dias sobre A. nerii e 2,8 e 4,8 dias sobre C. aonidum e a viabilidade de ovo a adulto foi de 63,2% e 62,7% respectivamente para indivíduos criados sobre essas presas. A longevidade de adultos de C. citricola foi de 57,0 dias com A. nerii e de 62,0 dias com C. aonidum. A proporção macho: fêmea foi de 0,82:1,00. O consumo diário de C. citricola foi de 1,15 adultos ou 2,35 ninfas de 2o ínstar ou 3,16 ninfas de 1o ínstar de A. nerii por dia.Laboratory trials were carried out to study biological aspects of Coccidophilus citricola Brèthes (Coleoptera: Coccinellidae on Chrysomphalus aonidum Linnaeus and Aspidiotus nerii Bouché (Homoptera: Diaspididae. The scales were reared on squashes and then served as food source to the predator. The period of egg, larvae (four ínstars, pre-pupae, pupae, adults’ longevity and male: female ratio was observed. Predatory efficacy of C. citricola on A. nerii was also determined. The period of egg was 7.9 and 8.1 days on A. nerii and C. aonidum. The development (days of different stages of C

  14. Viabilidade de sementes de trigo tratadas com fenitrotion e infestadas por Sitophilus oryzae (L. (Coleoptera: Curculionidae durante o armazenamento Viability of stored wheat seeds when treated with fenitrothion and infested by Sitophilus oryzae (L. (Coleoptera: Curculionidae

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    Isabel Helena de Almeida Zeituni Plazas

    2003-01-01

    Full Text Available O esclarecimento de aspectos do comportamento de gorgulhos (Sitophilus spp. em sementes de trigo armazenadas e o acompanhamento da qualidade física e fisiológica das sementes neste contexto, é essencial para aprimorar o monitoramento das sementes durante a armazenagem. Com o objetivo de avaliar a eficiência do inseticida fenitrotion, quanto à proteção oferecida às sementes de trigo contra os insetos Sitophilus oryzae (L. (Coleoptera: Curculionidae e de acompanhar a germinação das sementes tratadas ou não com inseticida, durante o armazenamento, sementes de trigo dos cultivares IAC-120 e IAC-350 foram tratadas ou não com fenitrotion três meses após a colheita e armazenadas em condições de ambiente de laboratório, em Campinas (SP, durante 15 meses. Em intervalos trimestrais, adultos de S. oryzae foram colocados nas amostras de sementes de cada uma das repetições dos tratamentos com insetos. Avaliou-se a sobrevivência desses insetos, bem como a progênie resultante. Em seguida, as sementes das amostras das repetições de todos os tratamentos com e sem insetos foram analisadas quanto ao grau de umidade, germinação e infestação por insetos. O inseticida fenitrotion, na dose recomendada pelo fabricante, não permitiu a proteção das sementes contra a infestação por S. oryzae, mesmo por períodos curtos de dois meses e que, na ausência de insetos, as sementes de trigo com elevado potencial de germinação inicial podem mantê-lo inalterado por períodos de 14 a 17 meses após a colheita, quando armazenadas em condições ambientes, em regiões com clima semelhante ao de Campinas.Clarifying aspects of the weevils (Sitophilus spp. behavior on stored wheat seeds, considering the seed quality, is essential to improve the seed management during storage. In order to evaluate the efficiency of the insecticide fenitrothion as protecting wheat seeds against Sitophilus oryzae (L. (Coleoptera: Curculionidae and to measure the seed

  15. Behavioral and Electroantennogram Responses of Plum Curculio, Conotrachelus nenuphar, to Selected Noxious Plant Extracts and Insecticides

    OpenAIRE

    Gökçe, A.; Stelinski, L. L.; Nortman, D. R.; Bryan, W. W.; Whalon, M. E.

    2014-01-01

    Behavioral and electroantennogram responses of plum curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), adults were tested for several methanolic plant extracts and organically approved insecticides. Plant extracts were evaluated for their potential as antifeedants or oviposition deterrents. These extract responses were also compared to those elicited by the non-neurotoxic, organic irritant-insecticide kaolin clay. Both sexes of plum curculio exhibited antennal response as ...

  16. Pepper Weevil (Coleoptera: Curculionidae) Preferences for Specific Pepper Cultivars, Plant Parts, Fruit Colors, Fruit Sizes, and Timing.

    Science.gov (United States)

    Seal, Dakshina R; Martin, Cliff G

    2016-01-01

    Peppers (Capsicum spp.) are an important crop in the USA, with about 32,000 ha cultivated in 2007, which resulted in $588 million in farm revenue. The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae), is the most troublesome insect pest of peppers in the southern United States. It is therefore urgent to find different vulnerabilities of pepper cultivars, fruit and plants parts, fruit colors and sizes, and timing to infestation by A. eugenii. Also relevant is testing whether fruit length and infestation state affect fruit numbers, weights, and proportions of fruit that are infested. Counts of A. eugenii adults and marks from oviposition and feeding suggested that C. chinense Jacquin "Habanero" was least susceptible, and C. annuum L. cultivars "SY" and "SR" were most susceptible. Comparison of plant parts and fruit sizes revealed that A. eugenii preferred the peduncle, calyx, and top of pepper fruits over the middle, bottom, leaves, or remainder of flowers. Anthonomus eugenii does not discriminate between green or yellow fruit color nor vary diurnally in numbers. Based on adult counts, medium to extra-large fruits (≥1.5 cm long) attracted more weevils than small fruits (<1.5 cm). However based on proportions of fruit numbers or fruit weights that were infested, there were no differences between large and small fruits. Choice of pepper cultivar can thus be an important part of an IPM cultural control program designed to combat A. eugenii by reduced susceptibility or by synchronous fruit drop of infested fruits. Our results are potentially helpful in developing scouting programs including paying particular attention to the preferred locations of adults and their sites of feeding and oviposition on the fruit. The results also suggested the potential value of spraying when the fruits are still immature to prevent and control infestation. PMID:26959066

  17. Taxonomic revision of Madagascan Rhantus (Coleoptera, Dytiscidae, Colymbetinae with an emphasis on Manjakatompo as a conservation priority

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    Anna Hjalmarsson

    2013-11-01

    Full Text Available We review the diving-beetle genus Rhantus Dejean of Madagascar (Coleoptera, Dytiscidae, Colymbetinae based on museum collection holdings and recently collected expedition material. Both morphology and DNA is used to test species boundaries, in particular whether newly collected material from the Tsaratanana mountains in the north represent a new species or are conspecific with Rhantus manjakatompo Pederzani and Rocchi 2009, described based on a single male specimen from the central Ankaratra mountains. DNA of the holotype of R. manjakatompo was successfully extracted in a non-destructive way and sequenced. The general mixed Yule coalescent model applied to an ultrametric tree constructed from mitochondrial cytochrome c oxidase subunit I (COI sequence data delimited three species. Morphological characters supported the same species unambiguously. We therefore recognise three species of Rhantus to occur in Madagascar: R. latus (Fairmaire, 1869, R. bouvieri Régimbart, 1900 and R. manjakatompo Pederzani and Rocchi, 2009. All three species are endemic to Madagascar and restricted to the highlands of the island. Rhantus stenonychus Régimbart, 1895, syn. n., is considered a junior synonym of R. latus. We designate lectotypes for R. bouvieri and R. goudoti Sharp, 1882, the latter a junior synonym of R. latus. We provide descriptions, a determination key, SEM-images of fine pronotal and elytral structures, distribution maps, habitus photos, and illustrations of male genitalia and pro- and mesotarsal claws. We discuss the role of the Manjakatompo forest as a refugium for Madagascan Rhantus diversity and other endemics of the montane central high plateau.

  18. Potential of two entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Coleoptera: Scarabaeidae), as biological control agents against the June beetle.

    Science.gov (United States)

    Erler, Fedai; Ates, A Ozgur

    2015-01-01

    The aim of this study was to evaluate the effectiveness of the entomopathogenic fungi (EPF), Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) strain PPRI 5339 [BroadBand, an emulsifiable spore concentrate (EC) formulation] and Metarhizium anisopliae (Metsch.) Sorokin (Hypocreales: Clavicipitaceae) strain F52 [Met52, both EC and granular (GR) formulations] against the larvae of Polyphylla fullo (L.) (Coleoptera: Scarabaeidae). Larvicidal bioassays were performed in foam boxes (100 by 75 by 50 cm; length by width by height), containing moist soil medium with some humus and potato tubers as food. Although the B. bassiana product (min. 4 × 10(9) conidia/ml) was applied at 100, 150, and 200 ml/100 l water; M. anisopliae strain F52 was applied at 500, 1,000, and 1,500 g/m(3) of moist soil medium for GR (9 × 10(8) cfu/g) and 75, 100, and 125 ml/100 l water for EC (5.5 × 10(9) conidia/ml) formulation. Both fungi were pathogenic to larvae of the pest; however, young larvae (1st and 2nd instars) were more susceptible to infection than older ones (3rd instar). Mortality rates of young and older larvae varied with conidial concentration of both fungi and elapsed time after application. The B. bassiana product was more effective than both of the formulations of the M. anisopliae product, causing mortalities up to 79.8 and 71.6% in young and older larvae, respectively. The highest mortality rates of young and older larvae caused by the M. anisopliae product were 74.1 and 67.6% for the GR formulation, 70.2 and 61.8% for the EC formulation, respectively. These results may suggest that both fungi have potential to be used for management of P. fullo. PMID:25881632

  19. Determinants of acute mortality of Hippodamia convergens (Coleoptera: Coccinellidae) to ultra-low volume permethrin used for mosquito management

    Science.gov (United States)

    Preftakes, Collin J.; Bodin, Jennifer L.; Brown, Christopher R.; Piccolomini, Alyssa M.; Schleier, Jerome J.

    2016-01-01

    There are relatively few experimental studies and risk assessments of the effects on non-target insects from ultra-low volume (ULV) insecticides used for management of adult mosquitoes. Therefore, we evaluated factors that may influence the ability of an insect to intercept the insecticide at the time of application by using Hippodamia convergens (Coleoptera: Coccinellidae) in field bioassay experiments in 2011 and 2015. Treatment factors included different distances, two cage heights (ground-level and 1.5 m above ground) to the point of the application, and covered vs. uncovered cage faces (2015 only). Insecticides used included a water-based formulation (Aqua-Reslin®) and an oil-based formulation (Permanone® 30-30) of permethrin. Cage height was highly significant both years, with much less acute (i.e., short-term exposure) mortality at ground-level compared with 1.5 m. In 2011, acute mortality was less at ground-level (mean = 3.2%, median = 0%) compared to 1.5 m (mean = 85.2%, median = 100%). Cage type also was highly significant, with less mortality in covered cages compared to uncovered cages. Mortality by cage height and cage type was as follows: ground level, covered cage (mean = 2.8%, median = 0.1%); ground level, uncovered cage (mean = 41.9%, median = 9.6%); 1.5 m, covered cage (mean = 6.8%, median = 0%); 1.5 m, uncovered cage (mean = 83.7%, median = 100%). Results suggest that acute mortality to non-target insects may vary considerably based on their height and their ability to directly intercept the insecticide as the aerosol passes through the area being sprayed. PMID:27366655

  20. Predaceous water beetles (Coleoptera, Hydradephaga) of the Lake St Lucia system, South Africa: biodiversity, community ecology and conservation implications.

    Science.gov (United States)

    Perissinotto, Renzo; Bird, Matthew S; Bilton, David T

    2016-01-01

    Water beetles are one of the dominant macroinvertebrate groups in inland waters and are excellent ecological indicators, reflecting both the diversity and composition of the wider aquatic community. The predaceous water beetles (Hydradephaga) make up around one-third of known aquatic Coleoptera and, as predators, are a key group in the functioning of many aquatic habitats. Despite being relatively well-known taxonomically, ecological studies of these insects in tropical and subtropical systems remain rare. A dedicated survey of the hydradephagan beetles of the Lake St Lucia wetlands (South Africa) was undertaken between 2013 and 2015, providing the first biodiversity census for this important aquatic group in the iSimangaliso Wetland Park, a UNESCO World Heritage Site within the Maputaland biodiversity hotspot. A total of 32 sites covering the entire spectrum of waterbody types were sampled over the course of three collecting trips. The Lake St Lucia wetlands support at least 68 species of Hydradephaga, a very high level of diversity comparing favourably with other hotspots on the African continent and elsewhere in the world and a number of taxa are reported for South Africa for the first time. This beetle assemblage is dominated by relatively widespread Afrotropical taxa, with few locally endemic species, supporting earlier observations that hotspots of species richness and centres of endemism are not always coincident. Although there was no significant difference in the number of species supported by the various waterbody types sampled, sites with the highest species richness were mostly temporary depression wetlands. This contrasts markedly with the distribution of other taxa in the same system, such as molluscs and dragonflies, which are most diverse in permanent waters. Our study is the first to highlight the importance of temporary depression wetlands and emphasises the need to maintain a variety of wetland habitats for aquatic conservation in this biodiverse

  1. Host Resistance of Five Fraxinus Species to Agrilus planipennis (Coleoptera: Buprestidae) and Effects of Paclobutrazol and Fertilization.

    Science.gov (United States)

    Tanis, Sara R; Mccullough, Deborah G

    2015-04-01

    Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) has killed millions of Fraxinus spp. trees in North America. While all Fraxinus species assessed to date can be colonized, A. planipennis attraction to host trees varies among species and with tree health. We established a plantation of 105 trees (21 trees each of four North American species Fraxinus americana L., Fraxinus nigra Marshall, Fraxinus pennsylvanica Marshall, Fraxinus quadrangulata Michaux, and the Asian species, Fraxinus mandshurica Ruprecht), and determined whether resistance to A. planipennis could be enhanced by fertilizer or paclobutrazol applications. Differences among species overshadowed most treatment effects. In 2010, A. planipennis survival over 14 d was 53% when beetles were caged with F. nigra, 30-32% when beetles were caged with F. americana, F. pennsylvanica, or F. mandshurica, and only 14% for beetles caged with F. quadrangulata. In 2011, beetle survival was lower for beetles caged with F. quadrangulata (33%) than F. americana (72%) or F. mandshurica (80%). In 2010 and 2011, leaf weight consumed by beetles was the same among Fraxinus species. However, beetles caged on F. quadrangulata consumed less leaf area than that by beetles caged with other ash species. In 2011, when trees were exposed to wild A. planipennis, larval density (per m(2)) was highest on F. nigra (235.9 ± 36.41) and F. pennsylvanica (220.1 ± 39.77), intermediate on F. americana (40.7 ± 11.61), and lowest on F. quadrangulata and F. mandshurica (2.0 ± 0.98 and 1.5 ± 0.67, respectively). Results indicate F. quadrangulata and F. mandshurica were relatively resistant to A. planipennis, F. nigra and F. pennsylvanica were highly vulnerable, and F. americana was intermediate. PMID:26313182

  2. All European Ash Species are Susceptible to Emerald Ash Borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae – a Far Eastern Invader

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    Yu. N. Baranchikov

    2014-12-01

    Full Text Available Emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae – an example of a destructive invasive insect which abruptly enlarged its initially East-Asian area in 80–90s of the previous century. Nowadays this species is the main pest of ash (Fraxinus trees in USA and Canada and is quickly spreading over 11 administrative regions of Russia. It is very important to determine a list of possible host plants of A. planipennis for the pest risk assessment of invasion of this pest over the territory of Central and Western Europe. In its native area – North-Eastern Asia – this buprestid is a secondary consumer of dying trees of East-Asian ash species F. сhinensis and F. mandshurica. Healthy trees of these species are highly resistant to the pest. No examples of resistant ash species were found at North American continent. Documentary data are presented for the first time on infestation of three European ash species at the territory of the Main Botanical Garden of the Russian Academy of Sciences in Moscow, Russia by the emerald ash borer. At the first time this pest was registered at the garden in 2011. During the period of 2010–2014 EAB killed from 70 to 100 % of trees of European ash species: Fraxinus excelsior, F. angustifolia (= F. oxycarpa and F. ornus. At the same period from 81 to 90 % of specimens of North American ash species (F. pennsylvanica and F. americana were killed by this buprestid. Simultaneously dead trees of Asian species F. mandshurica and F. chinensis (= F. rhynchophyla did not carry any trace of EAB infestation. This case study is a good example of the «sentinel trees» concept’s usefulness. Arboretums with collections of non-native plants may serve as «ecological traps» for the local pests and pathogens – potential invasive organisms in the source regions of introduced plants.

  3. Effects of host plant and larval density on intraspecific competition in larvae of the emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Duan, Jian J; Larson, Kristi; Watt, Tim; Gould, Juli; Lelito, Jonathan P

    2013-12-01

    Competition for food, mates, and space among different individuals of the same insect species can affect density-dependent regulation of insect abundance or population dynamics. The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a serious invasive pest of North American ash (Fraxinus spp.) trees, with its larvae feeding in serpentine galleries between the interface of sapwood and phloem tissues of ash trees. Using artificial infestation of freshly cut logs of green ash (Fraxinus pennsylvanica Marshall) and tropical ash (Fraxinus uhdei [Wenzig] Lingelsh) with a series of egg densities, we evaluated the mechanism and outcome of intraspecific competition in larvae of A. planipennis in relation to larval density and host plant species. Results from our study showed that as the egg densities on each log (1.5-6.5 cm in diameter and 22-25 cm in length) increased from 200 to 1,600 eggs per square meter of surface area, larval survivorship declined from ≍68 to 10% for the green ash logs, and 86 to 55% for tropical ash logs. Accordingly, larval mortality resulting from cannibalism, starvation, or both, significantly increased as egg density increased, and the biomass of surviving larvae significantly decreased on both ash species. When larval density was adjusted to the same level, however, larval mortality from intraspecific competition was significantly higher and mean biomasses of surviving larvae was significantly lower in green ash than in tropical ash. The role of intraspecific competition of A. planipennis larvae in density-dependent regulation of its natural population dynamics is discussed. PMID:24280666

  4. Predaceous water beetles (Coleoptera, Hydradephaga) of the Lake St Lucia system, South Africa: biodiversity, community ecology and conservation implications

    Science.gov (United States)

    Perissinotto, Renzo; Bird, Matthew S.; Bilton, David T.

    2016-01-01

    Abstract Water beetles are one of the dominant macroinvertebrate groups in inland waters and are excellent ecological indicators, reflecting both the diversity and composition of the wider aquatic community. The predaceous water beetles (Hydradephaga) make up around one-third of known aquatic Coleoptera and, as predators, are a key group in the functioning of many aquatic habitats. Despite being relatively well-known taxonomically, ecological studies of these insects in tropical and subtropical systems remain rare. A dedicated survey of the hydradephagan beetles of the Lake St Lucia wetlands (South Africa) was undertaken between 2013 and 2015, providing the first biodiversity census for this important aquatic group in the iSimangaliso Wetland Park, a UNESCO World Heritage Site within the Maputaland biodiversity hotspot. A total of 32 sites covering the entire spectrum of waterbody types were sampled over the course of three collecting trips. The Lake St Lucia wetlands support at least 68 species of Hydradephaga, a very high level of diversity comparing favourably with other hotspots on the African continent and elsewhere in the world and a number of taxa are reported for South Africa for the first time. This beetle assemblage is dominated by relatively widespread Afrotropical taxa, with few locally endemic species, supporting earlier observations that hotspots of species richness and centres of endemism are not always coincident. Although there was no significant difference in the number of species supported by the various waterbody types sampled, sites with the highest species richness were mostly temporary depression wetlands. This contrasts markedly with the distribution of other taxa in the same system, such as molluscs and dragonflies, which are most diverse in permanent waters. Our study is the first to highlight the importance of temporary depression wetlands and emphasises the need to maintain a variety of wetland habitats for aquatic conservation in this

  5. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations.

    Science.gov (United States)

    Beaudoin-Ollivier, L; Flori, A; Coffi, A; Cros, D; Glitho, I; Nodichao, L

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineensis × E. oleifera) × E. guineensis backcrosses. Females laid their eggs similarly on all breeding populations, with a preference for the E. guineensis La Mé origin. The average hatching rate reached 80% for the La Mé origin as opposed to 28% for the Deli origin. The mortality rates for the larval instars were greater on E. oleifera, on certain backcrosses and on the Deli origin of E. guineensis. Development at the second- and third- larval instars was the most affected, with a mortality rate of three to five times greater than that seen on La Mé. Epidermis and cuticle measurements indicated which breeding populations were suitable or unsuitable for the development of C. lameensis. E. guineensis, with its thin epidermis (12 µm) and cuticle (2 µm), proved to be highly susceptible to C. lameensis attacks. On the other hand, E. oleifera, which is very resistant, exhibited a thicker epidermis (17 µm) and cuticle (4 µm). The breeding populations were thus classified according to the positive or negative influence they exerted on the insect's egg laying and feeding. PMID:26113512

  6. Virulência de nematóides entomopatogênicos (Nematoda: Rhabditida a Sphenophorus levis (Coleoptera: Curculionidae

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    Fernando Henrique Carvalho Giometti

    2011-01-01

    Full Text Available O bicudo da cana-de-açúcar, Sphenophorus levis (Coleoptera: Curculionidae, é uma importante praga de solo dos canaviais no Estado de São Paulo, ocasionando prejuízos de até 30 t de cana/ha/ano. Visando ao controle biológico desta praga, esse estudo teve por objetivo avaliar a virulência de 17 isolados de nematóides entomopatogênicos do gênero Steinernema e Heterorhabditis contra adultos de S. levis, e o efeito dos quatro nematóides mais virulentos em três dosagens cada um. A pesquisa foi realizada em condições de laboratório, sendo realizados quatro ensaios para o estudo de virulência. Os nematóides selecionados como mais virulentos em cada teste foram S. brazilense IBCB n6, Heterorhabditis sp. IBCB n10, Heterorhabditis sp. IBCB n24 e Heterorhabditis sp. IBCB n44, sendo os únicos que diferenciaram significativamente das respectivas testemunhas com 45%, 40%, 20% e 31% de mortalidade do inseto na concentração de 240 juvenis infectivos (JI/inseto. No teste de dose, os isolados de Heterorhabditis identificados como IBCB n10, IBCB n24 e IBCB n44 destacaram-se quanto à virulência para os adultos do bicudo da cana-de-açúcar, diferindo significativamente da testemunha na maior dose avaliada (1200 JI/inseto com níveis de mortalidade do inseto de 60%, 65,7% e 74,3% respectivamente.

  7. Ácaros asociados al coleóptero Passalus cognatus (Coleoptera: Passalidae de Los Tuxtlas, Veracruz, México

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    Gabriel A Villegas-Guzmán

    2008-09-01

    Full Text Available Se revisaron 35 ejemplares de Passalus cognatus Truqui recolectados en Los Tuxtlas, Veracruz, México. En 20 de ellos se encontraron 245 ácaros pertenecientes a ocho especies, ocho géneros, ocho familias y tres subórdenes. Las especies de acáros más abundantes fueron Uroobovella californiana Wisniewski y Hirschmann (35%, Euzercon hyatti Hunter y Rosario (20% y Uropoda sp. (17.5%. Las zonas de fijación preferidas por los ácaros fueron las coxas; en menor proporción el mesoesternón y los húmeros. Los pasálidos infestados presentaron de 1 a 40 ácaros, con un promedio de 12. El número de especies de ácaros por pasálido infestado fue de 1 a 4.Mites associated to the Coleopteran Passalus cognatus (Coleoptera:Passalidae from Los Tuxtlas, Veracruz, Mexico. There are few records of mites associated with the tropical coleopterans of Mexico. We examined 35 passalid beetles (bessbugs Passalus cognatus from Los Tuxtlas region in Veracruz State, Mexico. Twenty of them had a total of 245 mites (representing eight species, eight genera, eight families and three suborders. The most abundant species were Uroobovella californiana Wisniewski & Hirschmann (35%, Euzercon hyatti Hunter & Rosario (20%, and Uropoda sp. (17.5%. The preferred attachment areas were the coxae; followed by the mesosternum and the humeri. Each beetle had 1 to 40 mites (average: 12; and we found 1-4 mite species per beetle. Rev. Biol. Trop. 56 (3: 1261-1268. Epub 2008 September 30.

  8. Coleoptera Scarabaeoidea de la región de Teziutlán, Puebla, México

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    Adrián MUÑOZ-HERNÁNDEZ

    2008-01-01

    Full Text Available Con el propósito de evaluar la riqueza, abundancia y fenología de las especies de Coleoptera Scarabaeoidea o Lamellicornia de la región de Teziutlán, Puebla, se realizaron colectas mensuales entre mayo de 1999 y junio de 2000, en sitios con bosques de pino-encino-liquidámbar o zonas de cultivo situados entre 1,990 y 2,000 m de altitud. Se capturaron 2,082 ejemplares que representan a 59 especies de 29 géneros, 18 tribus y 7 subfamilias de Melolonthidae, Scarabaeidae y Passalidae, entre las que destacan Phyllophaga godmani (Bates, Isonychus piperitus Bates, Anomala terroni Morón, Chrysina peruviana Kirby, Plusiotis aurofoveata Morón, Cyclocephala fasciolata Bates, Dynastes hyllus Chevrolat, Deltochilum mexicanum Bates, Ontherus mexicanus Harold, Onthophagus cyanellus Bates, Passalus punctatostriatus Percheron, Proculejus hirtus (Truqui y Odontotaenius zodiacus Truqui. La riqueza específica es elevada y representativa de ese tipo de bosques a pesar de la perturbación existente. Las especies más abundantes fueron Phyllophaga vetula (Horn, P. xanthe (Bates, Macrodactylus mexicanus Burmeister, Hoplia squamifera Burmeister, Cyclocephala jalapensis Casey, C. weidneri Endrodi, y Chrysina macropus Francillon. Los adultos de la mayor parte de las especies están activos entre abril y junio. De acuerdo con el índice de Simpson la fauna de lamelicornios de Teziutlán presenta un 80% de similitud con la fauna de las montañas del noreste de Hidalgo, un 33% con la región vecina de Cuetzalan, Puebla, y un 17% con el área de Los Tuxtlas, Veracruz. Se incluye una clave para separar las 59 especies encontradas.

  9. Coleoptera scarabaeoidea de la región de Teziutlán, Puebla, México

    Directory of Open Access Journals (Sweden)

    Adri\\u00E1n Mu\\u00F1oz Hern\\u00E1ndez

    2008-01-01

    Full Text Available Con el propósito de evaluar la riqueza, abundancia y fenología de las especies de Coleoptera Scarabaeoidea o Lamellicornia de la región de Teziutlán, Puebla, se realizaron colectas mensuales entre mayo de 1999 y junio de 2000, en sitios con bosques de pino-encino-liquidámbar o zonas de cultivo situados entre 1,990 y 2,000 m de altitud. Se capturaron 2,082 ejemplares que representan a 59 especies de 29 géneros, 18 tribus y 7 subfamilias de Melolonthidae, Scarabaeidae y Passalidae, entre las que destacan Phyllophaga godmani (Bates, Isonychus piperitus Bates, Anomala terroni Morón, Chrysina peruviana Kirby, Plusiotis aurofoveata Morón, Cyclocephala fasciolata Bates, Dynastes hyllus Chevrolat, Deltochilum mexicanum Bates, Ontherus mexicanus Harold, Onthophagus cyanellus Bates, Passalus punctatostriatus Percheron, Proculejus hirtus (Truqui y Odontotaenius zodiacus Truqui. La riqueza específica es elevada y representativa de ese tipo de bosques a pesar de la perturbación existente. Las especies más abundantes fueron Phyllophaga vetula (Horn, P. xanthe (Bates, Macrodactylus mexicanus Burmeister, Hoplia squamifera Burmeister, Cyclocephala jalapensis Casey, C. weidneri Endrodi, y Chrysina macropus Francillon. Los adultos de la mayor parte de las especies están activos entre abril y junio. De acuerdo con el índice de Simpson la fauna de lamelicornios de Teziutlán presenta un 80% de similitud con la fauna de las montañas del noreste de Hidalgo, un 33% con la región vecina de Cuetzalan, Puebla, y un 17% con el área de Los Tuxtlas, Veracruz. Se incluye una clave para separar las 59 especies encontradas.

  10. Biological aspects of Leucothyreus alvarengai Frey and Leucothyreus aff. semipruinosus Ohaus (Coleoptera, Melolonthidae, Rutelinae in crop succession at central Brazil

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    Alex Furquim Pereira

    2013-09-01

    Full Text Available Biological aspects of Leucothyreus alvarengai Frey and Leucothyreus aff. semipruinosus Ohaus (Coleoptera, Melolonthidae, Rutelinae in crop succession at central Brazil. Beetles of the family Melolonthidae make up a large group and some species are considered pests of planted crops. Little information is available on the basic biological aspects of the genus Leucothyreus, such as association with cultivated crops and their occurrence periods. Therefore studies were developed in soybean and corn crops in Tangará da Serra, Mato Grosso, Brazil, with the objective of studying the occurrence and biological aspects of Leucothyreus alvarengai Frey and Leucothyreus aff. semipruinosus Ohaus. For acquisition of immature specimens of both species, in April 2011 sampling was performed in corn fields, in July and October in the fallow area, and in soybeans fields planted in December; in 2012 sampling was performed in January and February in soybean fields and in March in corn fields. In 2011 the total number of larvae obtained in April, July, October and December were 100, 6, 30 and 27, and in January, February and March of 2012 these quantities were 32, 52 and 65 larvae, respectively. In all sampling events the larvae of L. alvarengai were collected in greater quantity. At the beginning of the reproductive period of L. alvarengai and L. aff. semipruinosus, it was observed that the adults began to fly and soon after started oviposition in the field in September. The appearance of larvae coincides with the time of soybean planting in the field, thus the larvae feed on roots of soybean plants at the beginning of their development and the cycle from egg to adult of the two species was completed in one year.

  11. The response of ground beetles (Coleoptera: Carabidae) to selection cutting in a South Carolina bottomland hardwood forest.

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael, D.; Hanula, James L.; Horn, Scott; Kilgo, John, C.; Moorman, Christopher, E.

    2005-04-01

    We compared the response of ground beetles (Coleoptera: Carabidae) to the creation of canopy gaps of different size (0.13, 0.26, and 0.50 ha) and age (1 and 7 years) in a bottomland hardwood forest (South Carolina, USA). Samples were collected four times in 2001 by malaise and pitfall traps placed at the center and edge of each gap, and 50 m into the surrounding forest. Species richness was higher at the center of young gaps than in old gaps or in the forest, but there was no statistical difference in species richness between old gaps and the forests surrounding them. Carabid abundance followed the same trend, but only with the exclusion of Semiardistomis viridis (Say), a very abundant species that differed in its response to gap age compared to most other species. The carabid assemblage at the gap edge was very similar to that of the forest, and there appeared to be no distinct edge community. Species known to occur in open or disturbed habitats were more abundant at the center of young gaps than at any other location. Generalist species were relatively unaffected by the disturbance, but one species (Dicaelus dilatatus Say) was significantly less abundant at the centers of young gaps. Forest inhabiting species were less abundant at the centers of old gaps than in the forest, but not in the centers of young gaps. Comparison of community similarity at various trapping locations showed that communities at the centers of old and young gaps had the lowest similarity (46.5%). The community similarity between young gap centers and nearby forest (49.1%) and old gap centers and nearby forest (50.0%) was similarly low. These results show that while the abundance and richness of carabids in old gaps was similar to that of the surrounding forest, the species composition between the two sites differed greatly.

  12. Effect of temperature and tree species on damage progression caused by whitespotted sawyer (Coleoptera: Cerambycidae) larvae in recently burned logs.

    Science.gov (United States)

    Bélanger, Sébastien; Bauce, Eric; Berthiaume, Richard; Long, Bernard; Labrie, Jacques; Daigle, Louis-Frédéric; Hébert, Christian

    2013-06-01

    The whitespotted sawyer, Monochamus scutellatus scutellatus (Say) (Coleoptera: Ce-rambycidae), is one of the most damaging wood-boring insects in recently burned boreal forests of North America. In Canada, salvage logging after wildfire contributes to maintaining the timber volume required by the forest industry, but larvae of this insect cause significant damage that reduces the economic value of lumber products. This study aimed to estimate damage progression as a function of temperature in recently burned black spruce (Picea mariana (Miller) Britton, Sterns, and Poggenburg) and jack pine (Pinus banksiana Lambert) trees. Using axial tomographic technology, we modeled subcortical development and gallery depth progression rates as functions of temperature for both tree species. Generally, these rates were slightly faster in black spruce than in jack pine logs. Eggs laid on logs kept at 12 degrees C did not hatch or larvae were unable to establish themselves under the bark because no larval development was observed. At 16 degrees C, larvae stayed under the bark for > 200 d before penetrating into the sapwood. At 20 degrees C, half of the larvae entered the sapwood after 30-50 d, but gallery depth progression stopped for approximately 70 d, suggesting that larvae went into diapause. The other half of the larvae entered the sapwood only after 100-200 d. At 24 and 28 degrees C, larvae entered the sapwood after 26-27 and 21 d, respectively. At 28 degrees C, gallery depth progressed at a rate of 1.44 mm/d. Temperature threshold for subcortical development was slightly lower in black spruce (12.9 degrees C) than in jack pine (14.6 degrees C) and it was 1 degrees C warmer for gallery depth progression for both tree species. These results indicate that significant damage may occur within a few months after fire during warm summers, particularly in black spruce, which highlights the importance of beginning postfire salvage logging as soon as possible to reduce economic

  13. Field trial of diatomaceous earth in cotton gin trash against the larger black flour beetle, Cynaeus angustus (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    McIntyre, N E; Porter, P

    2004-04-01

    The larger black flour beetle, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), is an agricultural and home nuisance pest in North America. In the Southern High Plains of Texas, the larger black flour beetle is associated with cotton gin trash, by-products of cotton ginning that are field stored in large piles for economic reasons. Larger black flour beetle overwinter in gin trash piles but may disperse by the millions in summer and autumn, entering houses as far as 2 km away where they cause distress to homeowners. Because > 1.2 billion kg of gin trash is produced annually in Texas alone, the potential consequences of the larger black flour beetle are enormous. We conducted a field experiment that evaluated the efficacy of diatomaceous earth (DE) on the abundance of the larger black flour beetle in gin trash. There were no significant differences in numbers of larger black flour beetle among treatments and controls (mean number of adults summed over time: controls = 115.41, layered treatment = 87.60, top and bottom treatment = 96.50, bottom treatment = 115.16). There were sufficient numbers of beetles in treated piles to still pose a potential home nuisance problem, likely because the moisture content of field-stored gin trash is too high for DE to work effectively. Therefore, treating cotton gin trash with diatomaceous earth will probably be unable to prevent home infestations of larger black flour beetle. Location within a gin trash pile and season influenced pest numbers, which has implications for long-term field storage of cotton gin trash. PMID:15154486

  14. The Ceratocanthinae of Ulu Gombak: high species richness at a single site, with descriptions of three new species and an annotated checklist of the Ceratocanthinae of Western Malaysia and Singapore (Coleoptera, Scarabaeoidea, Hybosoridae

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    Alberto Ballerio

    2010-01-01

    Full Text Available The remarkable species richness of Ceratocanthinae (Coleoptera: Scarabaeoidea: Hybosoridae found at Ulu Gombak (Selangor, West Malaysia, a secondary rainforest research station, is discussed. Eighteen species have been collected, mainly in nests of termites (Isoptera and bess beetles (Coleoptera: Passalidae. Among them at least seven are new species, three of them here described: Madrasostes hashimi sp. n., Madrasostes mirificum sp. n., and Pterorthochaetes tsurui sp. n. Four other species (Madrasostes agostii Paulian, Madrasostes clypeale Paulian, Madrasostes depressum Paulian, and Madrasostes simplex Paulian are recorded for the first time for West Malaysia and three for new states within West Malaysia (Pterorthochaetes insularis Gestro, Madrasostes malayanum Paulian and Madrasostes sculpturatum Paulian. A checklist of the 34 Ceratocanthinae recorded so far from West Malaysia and Singapore is provided with taxonomic, distributional and morphological remarks on some species.

  15. Los Escarabeidos y Geotrúpidos de la Comunidad de Madrid: lista de especies, distribución geográfica y patrones de diversidad (Coleoptera, Scarabaeoidea, Scarabaeidae y Geotrupidae

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    Lobo, J. M.

    2006-05-01

    Full Text Available In this work we summarize the main characteristics of Geotrupidae and Scarabaeidae (Coleoptera, Scarabaeoidea fauna inhabiting Comunidad de Madrid, including an updated checklist of species as well as maps representing the observed and potential distributions of all species. Geographical variation in species richness, rarity or endemicity was also estimated, with a brief discussion on the geographical pattern obtained.

    En este trabajo se resumen las características fundamentales de la fauna de Geotrupidae y Scarabaeidae (Coleoptera, Scarabaeoidea que habita la Comunidad de Madrid, presentándose un inventario actualizado así como mapas que representan la distribución observada y potencial de todas las especies. Finalmente, se estima la variación geográfica de la riqueza de especies, la rareza o la endemicidad, discutiendo brevemente el patrón geográfico encontrado.

  16. Flutuação Populacional de Rhynchophorus palmarum L. (Coleoptera: Curculionidae em Plantios de Palma de Óleo em Roraima

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    2015-08-01

    Full Text Available Esta pesquisa teve como objetivo avaliar a flutuação populacional de Rhynchophorus palmarum L. (Coleoptera: Curculionidae em plantios de Elaeis guineensis Jacq, em ecossistemas de savana e floresta no estado de Roraima. A pesquisa foi desenvolvida durante o período de janeiro de 2011 a fevereiro de 2012 nos campos experimentais da Embrapa Roraima: Monte Cristo e Caroebe, numa área de 2 hectares com a cultura da Palma de Óleo, em cada campo. Foram distribuídas armadilhas iscadas com roletes de cana-de-açúcar e feromônio de agregação para captura dos insetos no entorno dos plantios. Ocorreu flutuação de R. palmarum com picos populacionais nos meses de julho e agosto no ecossistema de savana e de abril a setembro no ambiente de floresta. A população de R. palmarum foi menor em meses de baixa precipitação pluviométrica nos dois ambientes avaliados com a Palma de Óleo em Roraima Population Fluctuation of Rhynchophorus palmarum L. (Coleoptera: Curculionidae in Plantations of Oil Palm in Roraima Abstract. This research aimed to evaluate the population fluctuation Rhynchophorus palmarum L. (Coleoptera: Curculionidae in Elaeis guineensis Jacq plantations in forest and savanna ecosystems in the state of Roraima. The research was conducted during the period from January 2011 to February 2012 in the experimental field of Embrapa Roraima: Monte Cristo and Caroebe in an area of 2 hectares with culture Oil Palm in each field. Traps baited with rollers cane sugar and aggregation pheromone for capturing insects in the vicinity of the plantations were distributed. Fluctuation occurred R. palmarum with population peaks in the months of July and August in the savanna ecosystem and from April to September in the forest environment. The population of R. palmarum was lower in months of low rainfall in the two environments assessed with a palm-oil-in Roraima.

  17. South American Coccinellidae (Coleoptera) : part 7, new name for Cyra Mulsant, review of Brachiacanthini genera, and systematic revision of Cleothera Mulsant, Hinda Mulsant and Serratitibia Gordon and Canepari, new genus

    OpenAIRE

    Gordon, Robert D.; Canepari, Claudio; Hanley, Guy A.

    2013-01-01

    Genera of Brachiacanthini (Coleoptera: Coccinellidae: Hyperaspidinae) are discussed and a key to all recognized genera provided. Cyrea, new genus, is proposed, and Serratitibia, new genus, is erected and revised, Cleothera Mulsant and Hinda Mulsant are recognized as valid genera and revised. Helesius caseyi Sicard is transferred to Hinda and recognized as a synonym of Hinda designata Mulsant, new synonymy. Brachiacantha brethesi (Korschefsky), Cleothera abendrothi Kirsch, Cleothera ambigua Mu...

  18. Natural history, ecology, and conservation of the genus Polyphylla Harris, 1841. 1. New species from the southwestern United States and Baja California, Mexico, with notes on distribution and synonymy (Coleoptera: Scarabaeidae: Melolonthinae)

    OpenAIRE

    La Rue, Delbert A.

    2016-01-01

    Four new species of the genus Polyphylla Harris (Coleoptera: Scarabaeidae: Melolonthinae) from the southwestern United States and Baja California, Mexico, are described and illustrated: Polyphylla anivallis, P. koso, P. morroensis, and P. socorriana. Two nomenclatural changes are proposed: Polyphylla ratcliffei Young is placed into synonymy with P. avittata Hardy and Andrews, new synonymy; P. uteana Tanner is removed from synonymy and reinstated as a valid species, reinstated status. The fema...

  19. Biology and Host-Range Testing of Laricobius kangdingensis sp. n. (Coleoptera: Derodontidae), a Newly Discovered Predator of Hemlock Woolly Adelgid, Adelges tsugae Annand (Homoptera: Adelgidae)

    OpenAIRE

    Gatton, Holly A.

    2005-01-01

    The biology and host-specificity of Laricobius kangdingensis sp. n. (Coleoptera: Derodontidae), a new predator of hemlock woolly adelgid (HWA), Adelges tsugae Annand (Homoptera: Adelgidae) were investigated and compared with that of a related predator of HWA, L. nigrinus Fender. Adults became active in June 2003; the males emerged first and in greater numbers than the females. The sex ratio of female to male was 1: 1.1. Laricobius kangdingensis sp. n. has four larval instars. The total nu...

  20. Una nueva especie de Meloe Linnaeus, 1758 del suroeste de Marruecos incluida en el grupo de M. (Eurymeloe) rugosus Marsham, 1802 (Coleoptera: Meloidae)

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    JOSÉ L. RUIZ; García-París, M.

    2015-01-01

    The scientific collections of the Museo Nacional de Ciencias Naturales (MNCN-CSIC, Madrid) hold an extense set of entomological materials collected in Morocco along the first decades of the XXth century by the preeminent naturalist M. Martínez de la Escalera. Morphological studies of the specimens of the genus Meloe Linnaeus, 1758 (Coleoptera: Meloidae) reveals the existence of populations morphologically differentiated along the coastal regions of Essaouira and Ifni. These populations are in...

  1. Bioactivity of Powder and Extracts from Garlic, Allium sativum L. (Alliaceae) and Spring Onion, Allium fistulosum L. (Alliaceae) against Callosobruchus maculatus F. (Coleoptera: Bruchidae) on Cowpea, Vigna unguiculata (L.) Walp (Leguminosae) Seeds

    OpenAIRE

    Abiodun A. Denloye

    2010-01-01

    Laboratory bioassays were conducted to investigate the bioactivity of powders, extracts, and essential oils from Allium sativum L. (Alliaceae) and A. fistulosum L. (Liliaceae) against adults, eggs, and larvae of Callosobruchus maculatus F. (Coleoptera: Bruchidae). On the basis of 48 hr median lethal toxicity (LC50), test plant powders and extracts from A. sativum were more toxic to C. maculatus adults than those from A. fistulosum. The 48 hr LC50 values for the powder against the test insect ...

  2. Eucnemid larvae of the Nearctic region. Part V: Fifth instar larval descriptions for eight species of Microrhagus Dejean, 1833 (Coleoptera: Eucnemidae: Melasinae: Dirhagini), with descriptions of four new species and notes on their biology

    OpenAIRE

    Otto, Robert L.

    2016-01-01

    Fifth instars for eight of the nine species of Microrhagus Dejean (Coleoptera: Eucnemidae: Melasinae: Dirhagini), all endemic in the Nearctic region, are described and illustrated. Biological information is provided, along with a brief discussion of the larval morphology among the eight Nearctic species of Microrhagus. Adult descriptions are provided for four new species. These new species include Microrhagus breviangularis new species, Microrhagus carinicollis new species, Microrhagus ...

  3. Fauna de Coleoptera no Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brasil: abundância e riqueza das famílias capturadas através de armadilhas de solo

    OpenAIRE

    Marinoni Renato C.; Ganho Norma G.

    2003-01-01

    This paper is a part of the studies on the Coleoptera fauna from Vila Velha, Ponta Grossa, Parana gathered through malaise and pitfall traps in sites with different floristic conditions. The present study deal with the data of pitfall trap captures, installed close to the malaise traps. The data were obtained weekly (52 samples), from September 1999 to August 2000. This survey was carried out on five areas, three of them with different plant succession stages (initial, intermediate, and advan...

  4. Dispersal of individuals of the flightless grassland ground beetle, Carabus hungaricus (Coleoptera: Carabidae), in three populations and what they tell us about mobility estimates based on mark-recapture

    Czech Academy of Sciences Publication Activity Database

    Elek, Z.; Drag, Lukáš; Pokluda, Pavel; Čížek, Lukáš; Bérces, S.

    2014-01-01

    Roč. 111, č. 5 (2014), s. 663-668. ISSN 1210-5759 Grant ostatní: Hungarian Academy of Sciences(HU) BO/00045/11/8; European Social Fund(CZ) CZ.1.07/2.3.00/20.0064 Institutional support: RVO:60077344 Keywords : Coleoptera * Carabidae * Carabus hungaricus Subject RIV: EH - Ecology, Behaviour Impact factor: 0.975, year: 2014 http://www.eje.cz/pdfs/eje/2014/05/10.pdf

  5. Litteratura Coleopterologica (1758–1900): a guide to selected books related to the taxonomy of Coleoptera with publication dates and notes

    Science.gov (United States)

    Bousquet, Yves

    2016-01-01

    Abstract Bibliographic references to works pertaining to the taxonomy of Coleoptera published between 1758 and 1900 in the non-periodical literature are listed. Each reference includes the full name of the author, the year or range of years of the publication, the title in full, the publisher and place of publication, the pagination with the number of plates, and the size of the work. This information is followed by the date of publication found in the work itself, the dates found from external sources, and the libraries consulted for the work. Overall, more than 990 works published by 622 primary authors are listed. For each of these authors, a biographic notice (if information was available) is given along with the references consulted. PMID:27231436

  6. A novel 8.7 kDa protease inhibitor from chan seeds (Hyptis suaveolens L.) inhibits proteases from the larger grain borer Prostephanus truncatus (Coleoptera: Bostrichidae).

    Science.gov (United States)

    Aguirre, Cesar; Valdés-Rodríguez, Silvia; Mendoza-Hernández, Guillermo; Rojo-Domínguez, Arturo; Blanco-Labra, Alejandro

    2004-05-01

    A novel trypsin inhibitor purified from chan seeds (Hyptis suaveolens, Lamiaceae) was purified and characterized. Its apparent molecular mass was 8700 Da with an isoelectric point of 3.4. Its N-terminal sequence showed a high content of acidic amino acids (seven out of 18 residues). Its inhibitory activity was potent toward all trypsin-like proteases extracted from the gut of the insect Prostephanus truncatus (Coleoptera: Bostrichidae), a very important pest of maize. This activity was highly specific, because among proteases from seven different insects, only those from P. truncatus and Manduca sexta (Lepidoptera: Sphingidae) were inhibited. This inhibitor has potential to enhance the defense mechanism of maize against the attack of P. truncatus. PMID:15142539

  7. Effet structurant de la plante hôte chez la bruche de l'arachide, Caryedon serratus (Olivier, 1790 (Coleoptera : Bruchidae

    Directory of Open Access Journals (Sweden)

    Sembène, M.

    2012-01-01

    Full Text Available Structuring effect of the host plant in the groundnut bruchid, Caryedon serratus (Olivier, 1790 (Coleoptera: Bruchidae. Twenty-six samples of the groundnut seed-beetle which were reared from pods of five different host plants (Arachis hypogaea L., Bauhinia rufescens Lam., Cassia sieberiana DC., Piliostigma reticulatum (DC. Hochst. and Tamarindus indica L. in four localities of Senegal were compared using electrophoresis based on six loci of four enzymatic systems. The population structure of Caryedon serratus Olivier was analysed using Weir and Cockerham's estimator of Wright's F-statistics. θ value (0.235 and the dendrogram of Rogers'genetic distances revealed a high degree of genetic differentiation between host plants. Genetic analysis without C. sieberiana samples indicated that populations form host races which are partially isolated according to their host plants (θ = 0.035. Geographical distances between localities are not decisive for genetic structuration of C. serratus populations from a given host plant.

  8. Flea beetles (Coleoptera: Chrysomelidae: Alticinae collected by malaise trap method in Gölcük Natural Park (Isparta, Turkey, with a new record for Turkish fauna

    Directory of Open Access Journals (Sweden)

    Aslan Gül E.

    2012-01-01

    Full Text Available This study is based on Alticinae (Coleoptera: Chrysomelidae material collected by Malaise trapping which is different from other standardized collecting methods. A total of 19 flea beetle species belonging to 6 genera were collected from Gölcük Natural Park, Isparta (Turkey during 2009. The species are listed in a table together with distributional data in Turkey. Among them, Longitarsus curtus (Allard, 1860 is recorded for the first time in Turkey. L. monticola Kutschera, 1863 and L. curtus are recently separated synonyms and thus all data referring to the distribution of both species are currently important. Hence, the zoogeographical distribution of the new record is reviewed with some remarks; habitus and genitalia are illustrated.

  9. Elemental distribution in reproductive and neural organs of the Epilachna nylanderi (Coleoptera: Coccinellidae), a phytophage of nickel hyperaccumulator Berkheya coddii (Asterales: Asteraceae) by micro-PIXE.

    Science.gov (United States)

    Mesjasz-Przybyłowicz, Jolanta; Orłowska, Elżbieta; Augustyniak, Maria; Nakonieczny, Mirosław; Tarnawska, Monika; Przybyłowicz, Wojciech; Migula, Paweł

    2014-01-01

    The phenomenon of metal hyperaccumulation by plants is often explained by a pathogen or herbivore defense hypothesis. However, some insects feeding on metal hyperaccumulating plants are adapted to the high level of metals in plant tissues. Former studies on species that feed on the leaves of Berkheya coddii Roessler 1958 (Asteraceae), a nickel-hyperaccumulating plant, demonstrated several protective mechanisms involved in internal distribution, immobilization, and elimination of Ni from the midgut and Malpighian tubules. These species are mainly coleopterans, including the lady beetle, Epilachna nylanderi (Mulsant 1850) (Coleoptera: Coccinellidae), collected from the ultramafic ecosystem near Barberton in South Africa. By performing particle-induced X-ray emission microanalysis elemental microanalysis (PIXE), this study examined whether Ni may be harmful to internal body systems that decide on insect reactivity (central nervous system [CNS]), their reproduction, and the relationships between Ni and other micronutrients. Data on elemental distribution of nine selected elements in target organs of E. nylanderi were compared with the existing data for other insect species adapted to the excess of metals. Micro-PIXE maps of seven regions of the CNS showed Ni mainly in the neural connectives, while cerebral ganglia were better protected. Concentrations of other bivalent metals were lower than those of Ni. Testis, compared with other reproductive organs, showed low amounts of Ni. Zn was effectively regulated at physiological dietary levels. In insects exposed to excess dietary Zn, it was also accumulated in the reproductive organs. Comparison of E. nylanderii with other insects that ingest hyperaccumulating plants, especially chrysomelid Chrysolina clathrata (Clark) (Coleoptera: Chrysomelidae), showed lower protection of the CNS and reproductive organs. PMID:25399425

  10. Leaves of Lantana camara Linn. (Verbenaceae) as a potential insecticide for the management of three species of stored grain insect pests.

    Science.gov (United States)

    Rajashekar, Y; Ravindra, K V; Bakthavatsalam, N

    2014-11-01

    Insects cause extensive damage to stored grains and their value added products. Among the stored grain pests Sitophilus oryzae (L.) Callosobruchus chinensis (Fab.) and Tribolium castaneum (Herbst.) are considered as destructive pests in India. Plants may provide alternatives to currently used insect control agents as they constitute rich source in bioactive molecules. Lantana camara, an erect shrub, which grows widely in the tropics, exhibits insecticidal activity against several insects. The methanol extract from leaves of L. camara has fumigant and contact toxicity against S. oryzae, C. chinesis and T. castaneum. In fumigant assays, The LC50 for S. oryzae was 128 μl/L(1), C. chinensis 130.3 μl/L(1), and T. castaneum 178.7 μl/L(1). The LD50 values for S. oryzae C. chinensis and T. castaneum in contact toxicity were 0.158, 0.140 and 0.208 mg/cm(2), respectively. For grain treatment, a concentration of 500 mg/L(1) and 7 days exposure were needed to obtain 90 - 100 % population extinction in all three insects. Probit analysis showed that C. chinensis were more susceptible than S. oryzae and T. castaneum. Gaschromatography-Mass Spectrometer (GCMS) studies for extracts indicated the presence of potent fumigant molecules in L. camara. The prospect of utilizing L. camara as potent fumigant insecticide is discussed. PMID:26396352

  11. Research into the Application of the Sterile-Male Release Technique to the Rhinoceros Beetle (Coleoptera:Scarabaeidae)

    International Nuclear Information System (INIS)

    The control of the Rhinoceros beetle, which causes considerable damage in palm and coconut plantations in the tropical regions of the world, meets with difficulties resulting from the habits of the insects. Chemical and biological control methods have not yet produced economically worthwhile results. It was therefore proposed, under the Joint Project established by the UN Special Fund and the South Pacific Commission for devising a method of controlling the main pest in this group, Oryctes rhinoceros, that the possibility be investigated of using against these coleoptera the technique of releasing males sterilized by radiation. Studies have been made involving the two most important species O. monoceros Ol. and O. rhinoceros L. The first stage was the development of a continuous breeding method, which had not hitherto been possible because of the large-scale slaughter arising from fungus attacks by Metarrhizium anisopliae Metsch. For the larvae the technique used involved first individual rearing, followed by group rearing tests with 100 or so insects in tanks. The average temperature in both cases was close to 30°C and the insects were fed on a mixture of rotten wood and leaf mould mixed with the faeces of domestic animals. The adults, fed on banana slices, are placed in troughs or tanks containing sieved-earth. It was thus possible to rear Oryctes in several successive cycles, avoiding the epizooic stage of Metarrhizium, with a multiplication rate from one generation to the next of close on 10, Tests on radiation sterilization procedures taking as variables the gamma-ray dose, the stage of the insect and the physiological state of the imago, showed that O. monoceros adults were sterilized by a single application of 4000 rad, with a slight reduction in their length of life. The same was true of O. rhinoceros, which however required a dose greater than 6000 rad to sterilize it. The mating urge and sexual vigour of the irradiated insects are, however, reduced

  12. Simulação do dano causado por larvas de Oryzophagus oryzae (Coleoptera: Curculionidae a cultivares de arroz irrigado

    Directory of Open Access Journals (Sweden)

    Martins José Francisco da Silva

    2004-01-01

    Full Text Available Oryzophagus oryzae (Costa Lima, 1936 (Coleoptera: Curculionidae é a praga-chave da cultura do arroz irrigado na Região Sul do Brasil. As larvas causam os principais danos ao cortarem drasticamente as raízes das plantas. Dois experimentos sobre simulação do dano larval foram realizados testando um equipamento cujo componente principal é uma lâmina metálica em forma de U, para corte das raízes. Raízes das cultivares BR-Irga 414 e Bluebelle, de ciclo biológico curto, e suscetíveis ao inseto e BR-Irga 410 e Dawn, de ciclo médio e resistentes, foram submetidas aos tratamentos de (1 corte artificial, com o simulador, (2 corte natural, pelas larvas e (3 sem corte, artificial ou natural, protegidas com inseticida. A simulação foi praticada na fase vegetativa das plantas, 31 dias pós-irrigação por inundação, época do pico da população larval nas raízes. Avaliaram-se dados sobre comprimento, peso de matéria seca e volume de raízes, imediatamente após a aplicação do tratamento de simulação e na pré-emissão de panículas, população larval em plantas submetidas ao dano natural, e produtividade de grãos. Na avaliação efetuada imediatamente após a simulação, em todas as cultivares, não ocorreu diferença entre índices de dano às raízes resultantes do uso do simulador e da alimentação de larvas. Na fase de pré-emissão das panículas, os índices de recuperação do sistema radicular das plantas submetidas aos danos natural e artificial, também foram similares. As cultivares de ciclo médio apresentaram maior recuperação dos tecidos radiculares e menor perda de produtividade de grãos, confirmando o maior grau de resistência a O. oryzae. Concluiu-se que o método de simulação é aplicável em pesquisas sobre resistência de arroz ao inseto, principalmente na identificação de genótipos tolerantes.

  13. Prediction of the key binding site of odorant-binding protein of Holotrichia oblita Faldermann (Coleoptera: Scarabaeida).

    Science.gov (United States)

    Zhuang, X; Wang, Q; Wang, B; Zhong, T; Cao, Y; Li, K; Yin, J

    2014-06-01

    The scarab beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae) is a predominant underground pest in the northern parts of China, and its larvae (grubs) cause great economic losses because of its wide range of host plants and covert habitats. Environmentally friendly strategies for controlling adults would have novel and broad potential applications. One potential pest management measure is the regulation of olfactory chemoreception to control target insect pests. In the process of olfactory recognition, odorant-binding proteins (OBPs) are believed to carry hydrophobic odorants from the environment to the surface of olfactory receptor neurons. To obtain a better understanding of the relationship between OBP structures and their ligands, homology modelling and molecular docking have been conducted on the interaction between HoblOBP1 and hexyl benzoate in the present study. Based on the results, site-directed mutagenesis and binding experiments were combined to describe the binding sites of HoblOBP1 and to explore its ligand-binding mechanism. After homology modelling of HoblOBP1, it was found that the three-dimensional structure of HoblOBP1 consists of six α-helices and three disulphide bridges that connect the helices, and the hydrophobic pockets are both composed of five helices. Based on the docking study, we found that van der Waals interactions and hydrophobic interactions are both important in the bonding between HoblOBP1 and hexyl benzoate. Intramolecular residues formed the hydrogen bonds in the C terminus of the protein and the bonds are crucial for the ligand-binding specificity. Finally, MET48, ILE80 and TYR111 are binding sites predicted for HoblOBP1. Using site-directed mutagenesis and fluorescence assays, it was found that ligands could not be recognized by mutant of Tyr111. A possible explanation is that the compound could not be recognized by the mutant, and remains in the binding cavity because of the loss of the intramolecular

  14. EXTRATO AQUOSO DE FOLHAS DE Azadirachta indica A. Juss NO CONTROLE DE Sitophilus zeamais Mots. (COLEOPTERA: CURCULIONIDAE EM MILHO ARMAZENADO

    Directory of Open Access Journals (Sweden)

    Marcelo Toller Borsonaro

    2013-05-01

    Full Text Available The present work had as objective to test different dosages of aqueous extract of leaves of neem(Azadirachta indica as natural alternative and of easy application in the control of Sitophilus zeamais in grains of corn, seeing it is one of main stored corn pests. Were evaluated the following concentrations: 10%, 20%, 30% and witness. They were applied directly on the mass of grains and the insects with a pressurized pulverizer model Guarany PCP-1P in the dosage of 0,56 mL for dish. The treatments did not present significant difference as the reduction of weight of grains, but there was significant difference in relation to the population control. As for this parameter, the aqueous extract of leaves of neem in concentration 30,3%, can be considered an alternative treatment against S. zeamais in stored corn grains.O presente trabalho teve como objetivo testar diferentes dosagens de extrato aquoso de folhas de Nim (Azadirachta indica A Juss como alternativa natural e de fácil aplicação no controle de Sitophilus zeamais Mots.(Coleoptera: Curculionidae em grãos de milho (Zea mays L., visto que esta é uma das principais pragas do milhoarmazenado. Avaliaram-se as seguintes concentrações: 10%, 20%, 30,3%, além do tratamento testemunha. Foram aplicados diretamente sobre a massa de grãos e sobre os insetos com um pulverizador pressurizado modelo Guarany PCP-1P na dosagem de 0,56 mL por placa. Os tratamentos não apresentaram diferença significativa quanto à redução de peso de grãos, mas houve diferença significativa em relação ao controle populacional. Quanto a este parâmetro, o extrato aquoso de folhas de Nim na concentração 30,3%, pode ser considerado um tratamento alternativo no controle de S. zeamais em grãos de milho armazenado.

  15. ABUNDÂNCIA RELATIVA DAS ESPÉCIES DE CERAMBYCIDAE (INSECTA-COLEOPTERA EM POMAR DE FRUTÍFERAS MISTO RELATIVE ABUNDANCE OF SPECIES OF THE CERAMBYCIDAE FAMILY (INSECTA-COLEOPTERA IN MIXED ORCHARD

    Directory of Open Access Journals (Sweden)

    Elisabeth Rose Pereira da Silva

    2007-09-01

    Full Text Available

    Estudou-se a abundância relativa das espécies da família Cerambycidae (Insecta-Coleoptera em um pomar de frutíferas misto composto por 28 diferentes espécies, no período de dezembro de 1997 a maio de 1999 em Ceres, Estado de Goiás, Brasil. Foram utilizadas armadilhas luminosas modelo “Luiz de Queiroz” com lâmpadas Bl-15 wats. Essas armadilhas foram ligadas por 12 horas em dois dias consectivos num total de 24 horas de coletas semanais. Coletaram-se 1.474 cerambicídeos, agrupados em 39 gêneros e 49 espécies. Acanthoderes jaspidea, Achryson surinamum, Chlorida festiva, Eurodacrys sexgutatta, Gnomibidion fulvipes, Lophopoeum timbouve, Megacyllene acuta, Rhopalophora collaris e Trichophorus distinctus foram as espécies classificadas como muito abundantes. Das espécies coletadas, 48,98 % foram classificadas como raras, 12,24 % como dispersas, 20,41 % como comuns e 18,37 % como muito abundantes. Dentre os 39 gêneros, Oreodera foi representado por três espécies (6,13 %, os gêneros Acanthoderes, Aerenica, Chrysoprasis, Colobothea, Eutrypanus, Megacylene, Myoxomorpha e Nyssodrysternum por duas espécies (4,08 %, e os demais gêneros por apenas uma espécie.

    PALAVRAS-CHAVE: Levantamento; riqueza de espécies; ecologia; comportamento.

    It was studied the relative abundance to the species of the family Cerambycidae (Insecta-Coleoptera in a mixed orchard composed by 28 diferent species in the period from decembre 1997 to may 1999 in Ceres, state of Goi

  16. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    Science.gov (United States)

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineensis × E. oleifera) × E. guineensis backcrosses. Females laid their eggs similarly on all breeding populations, with a preference for the E. guineensis La Mé origin. The average hatching rate reached 80% for the La Mé origin as opposed to 28% for the Deli origin. The mortality rates for the larval instars were greater on E. oleifera, on certain backcrosses and on the Deli origin of E. guineensis. Development at the second- and third- larval instars was the most affected, with a mortality rate of three to five times greater than that seen on La Mé. Epidermis and cuticle measurements indicated which breeding populations were suitable or unsuitable for the development of C. lameensis. E. guineensis, with its thin epidermis (12 µm) and cuticle (2 µm), proved to be highly susceptible to C. lameensis attacks. On the other hand, E. oleifera, which is very resistant, exhibited a thicker epidermis (17 µm) and cuticle (4 µm). The breeding populations were thus classified according to the positive or negative influence they exerted on the insect’s egg laying and feeding. RÉSUMÉ. La comestibilité de différents matériels végétaux d’Elaeis sp. présents au Bénin est testée pour Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), important ravageur du palmier à huile en Afrique. Des expérimentations en manchons ont permis de déterminer les capacités d’oviposition des femelles et les taux de mortalité des différents stades de d

  17. Resistência de genótipos de batata (Solanum spp. a Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae Resistence of potatoes genotypes (Solanum spp. to Diabrotica speciosa (Germar,1824 (Coleoptera: Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Fernando Mesquita Lara

    2000-12-01

    Full Text Available Avaliou-se a resistência de genótipos de batata (Solanum spp. a Diabrotica speciosa (Germar,1824 em condições de campo e laboratório. O plantio de campo foi efetuado em julho de 1997, na FCAV/ UNESP, Campus de Jaboticabal, utilizando-se os seguintes genótipos: Achat, NYL 235-4, 288.759-16, 288. 771-6, 288.776-3, 288.776-6, 288.787-5, 288.801-8 e 288.814-7. Avaliaram-se o consumo de área foliar em levantamentos realizados aos 70 e 90 dias após plantio e os danos ocasionados pelas larvas aos tubérculos, após a colheita. Em laboratório, foram realizados testes de preferência para alimentação com plantas de 40 dias (teste sem chance de escolha, envolvendo os nove genótipos presentes no campo, e com plantas de 47 e 68 dias (com e sem chance de escolha, avaliando-se cinco genótipos. Constatou-se, no ensaio de campo, que os genótipos 288.759-16, 288.776-3, 288.776-6, 288.787-5 e 288.814-7 foram menos consumidos por adultos de D. speciosa que a cultivar Achat, porém, em laboratório, 288.759-16 e 288.776-6 não rev elaram essa característica. O consumo em NYL 235-4 e 288.801-8 foi intermediário, não diferindo dos demais. NYL 235-4 e 288.801-8 foram resistentes às larvas, apresentando, em média, menos que 3 furos por tubérculo. O genótipo 288.776-3 destacou-se como resistente a larvas e adultos de D. speciosa, sendo a resistência a adultos do tipo não-preferência para alimentação (antixenose.The resistance of potato genotypes to Diabroticaspeciosa (Germar,1824 (Coleoptera: Chrysomelidae, was evaluated under field and laboratory conditions. The field trial was set up in the experimental farm of FCAV - UNESP, Jaboticabal, SP, and the following genotypes were compared: Achat, NYL 235-4, 288.759-16, 288.771-6, 288.776-3, 288.776-6, 288.787-5, 288.801-8 e 288.814-7. The leaf area consumption by adults was evaluated at 70 and 90 days after planting, and, after harvest, larval damage to tubers (number of perforations was assessed

  18. Systematics and phylogenetics of Indo-Pacific Luciolinae fireflies (Coleoptera: Lampyridae) and the description of new genera.

    Science.gov (United States)

    Ballantyne, Lesly A; Lambkin, Christine L

    2013-01-01

    This revision completes a taxonomic survey of fireflies (Coleoptera: Lampyridae) in the area encompassed by Australia, the Republic of Palau, Federated States of Micronesia, Papua New Guinea, Indonesia (West Irian/Papua), Solomon Islands, New Caledonia, Vanuatu and Fiji. It finalises the taxonomic issues arising from the 1969–70 voyage of the scientific vessel Alpha Helix to New Guinea. The firefly fauna of this area is exclusively Luciolinae. The scope of the revision was extended to include all known Luciolinae genera and certain species from SE Asia, and a phylogenetic analysis of 436 morphological characters of males, females, and associated larvae includes 142 Luciolinae species (Ballantyne & Lambkin 2009, and Fu et al. 2012a). The phylogenetic analyses infer four major groups within the Luciolinae. The monotypic Missimia Ballantyne is sister to all remaining Luciolinae and forms a grade to Aquatica Fu etBallantyne. The large clade of Curtos Motschulsky, Photuroluciola Pic, Colophotia Motschulsky, Poluninius gen. nov., Pyrophanes Olivier, Pteroptyx s. str. Olivier, Medeopteryx gen. nov., Trisinuata gen. nov., and Australoluciola gen. nov.forms a grade to the clade of Luciola s. str. Laporte (including Bourgeoisia Olivier). The monotypic Emeia Fu et al.forms a grade with a clade of Luciola and Pygoluciola Wittmer, sister to a large clade of Convexa Ballantyne, Pacifica gen. nov., Magnalata Ballantyne, Lloydiella Ballantyne, Asymmetricata Ballantyne, Pygatyphella s. str. Ballantyne, Atyphella Olliff, Aquilonia Ballantyne, and Gilvainsula Ballantyne. Luciola is paraphyletic, found in up to six clades across the tree. Together with Luciola, Magnalata, Aquilonia, and Gilvainsula render Atyphella paraphyletic. The new genera described here are all monophyletic and supported in the phylogenetic analyses that also provide evidence for the inclusion of taxa within them. Twenty-three genera including five new ones, and ten new species, are recognised and keys are

  19. Střevlíkovití a drabčíkovití (Coleoptera, Staphylinidae) brouci NPR Brouskův mlýn a jejich využití pro biomonitorování stavu biotopů

    Czech Academy of Sciences Publication Activity Database

    Boháč, Jaroslav

    České Budějovice : Calla, 2003, s. 14-18. [Národní přírodní rezervace Brouskův mlýn. Borovany (CZ), 06.06.2003] R&D Projects: GA AV ČR KSK6005114 Keywords : Coleoptera * Staphylinidae * biomonitoring Subject RIV: EH - Ecology, Behaviour

  20. Distribución y caracterización morfológica de Hycleus duodecimmaculatus (Olivier, 1811 (Coleoptera, Meloidae, una especie poco conocida del oeste de Marruecos

    Directory of Open Access Journals (Sweden)

    Ruiz, José L.

    2014-06-01

    Full Text Available Hycleus duodecimmaculatus (Coleoptera, Meloidae, Mylabrini is a poorly known species endemic to Morocco. Its geographic range extends along the coastal-subcoastal fringe of the Atlantic Coast, between Larache and Tiznit region (Arbaa Ait Ahmed. In this paper we study more than a hundred specimens, mostly preserved in the collection of the Museo Nacional de Ciencias Naturales (MNCN-CSIC, Spain. We provide new geographic records, including the Western Rif and confirm its presence in Marrakech (northwestern slopes of the High Atlas. Because of the scarcity of information, disperse and sometimes erroneous, we synthesize information available on chorology and phenology, and characterize the typology of the species’ habitat. From a taxonomic point of view, we redefine the species’ diagnostic characters and illustrate various aspects of the intra- and interpopulational morphological variability.Hycleus duodecimmaculatus (Coleoptera, Meloidae, Mylabrini es un endemismo de Marruecos muy poco conocido, cuya distribución se extiende por la franja costera-subcostera del Marruecos atlántico, entre Larache y la región de Tiznit (Arbaa Ait Ahmed. En este trabajo, realizado principalmente a partir del estudio de más de un centenar de ejemplares conservados en su mayor parte en la colección del Museo Nacional de Ciencias Naturales (MNCN-CSIC, España, se aportan nuevas citas geográficas incluyendo el Rif occidental, y se confirma su presencia en Marrakech (falda noroccidental del Alto Atlas. A la vista de la escasez de información existente para esta especie, dispersa y a veces errónea, se sintetiza la información corológica disponible, y se ofrecen datos sobre fenología de los adultos y tipología general de los hábitats que ocupa. Desde el punto de vista taxonómico, se redefinen los caracteres diagnósticos de la especie y se ilustran diversos aspectos de la variabilidad morfológica intra e interpoblacional.