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Sample records for castaneum herbst coleoptera

  1. Efeitos da radiação gama do Cobalto-60 em ovos de Tribolium castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae Gamma radiation effects on eggs of Tribolium castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae

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    L. S. Fontes

    1994-12-01

    Full Text Available Este trabalho teve por objetivo determinar as doses esterilizantes e letal para ovos de T. castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae, através de doses crescentes de radiação gama. Utilizou-se uma fonte de Cobalto-60, tipo Gammabeam-650, com taxa de dose de 1,28 kGy/hora. O experimento foi conduzido sob condições controladas com temperatura de 25 ± 2°C e umidade relativa de 70 ± 5%. As doses letal e esterilizante para os ovos foram respectivamente 30 e 20 Gy.The objective of this research was to verify the effects of gamma radiation of a Cobalt-60 source on eggs of the red flour beetle Tribolium castaneum (Herbst., 1797. The used dose rate was 1.28 kGy per hour, and the irradiated insects were kept under controlled environment condition: 25 ± 2°C and 70 ± 5% relative humidity. For eggs the sterilizing and lethal dose were respectively, 20 and 30 Gy.

  2. Interaction of starvation and gamma radiation on the fecundity of tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

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    Khattak, S.U.K.; Shafique, M. (Nuclear Inst. for Agriculture and Biology, Faisalabad (Pakistan))

    Starved and unstarved beetles of Tribolium castaneum (Herbst) were irradiated with low doses of gamma radiation viz:-4, 6, 8, 12 krad and their fecundity was studied up to 8 days under controlled laboratory conditions. It was recorded that fecundity decreased immediately following irradiation, but the subsequent recovery was dose dependent. The rate of oviposition increased with an increased period of starvation. Pre-irradiation starved beetles were less fecunditive than the post-irradiation ones. Irradiated individuals, though, laid eggs, but none of the eggs hatched at 8 and 12 krad.

  3. Evaluation of Standard Loose Plastic Packaging for the Management of Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebriondiae)

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    Hassan, Muhammad Waqar; Gulraize; Ali, Usman; Ur Rehman, Fazal; Najeeb, Hafsa; Sohail, Maryam; Irsa, Bakhtawar; Muzaffar, Zubaria; Chaudhry, Muhammad Shafiq

    2016-01-01

    Three standard foodstuff plastic packaging namely polyethylene (PE), polypropylene (PP), and polyvinylchloride (PVC) were evaluated for management of lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Resistance parameters in packaging were recorded as punctures, holes, penetrations, sealing defects, and invasions with two thicknesses and tested for two lengths of time. Damages like punctures, holes and penetrations by both insects were more in PE packaging however R. dominica made more penetrations in PP than in PE. For both insects sealing defects and invasions were predominant in PVC than in others. Thickness did not affect significantly damage types but significantly more holes and penetrations by R. dominica were in less thickness. Punctures and holes by R. dominica were more after less time period but other damages in packaging were more after more time period. However for T. castaneum all sorts of damages were seen more after more time period. Overall categorization between two insects showed R. dominica made more penetrations and T. castaneum made more invasions compared with their counterparts. Pictures were taken under camera fitted microscope to magnify punctures and holes in different packaging and thicknesses. Insect mortality due to phosphine was more in PP and PE packaging and least in PVC packaging and thickness effect was marginal. T. castaneum mortality was significantly more after 48 h than after 24 h. Damages extent in packaging and fumigation results showed PP to be the best of three packaging materials to manage these insects. PMID:27638958

  4. Evaluation of Standard Loose Plastic Packaging for the Management of Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebriondiae).

    Science.gov (United States)

    Hassan, Muhammad Waqar; Gulraize; Ali, Usman; Ur Rehman, Fazal; Najeeb, Hafsa; Sohail, Maryam; Irsa, Bakhtawar; Muzaffar, Zubaria; Chaudhry, Muhammad Shafiq

    2016-01-01

    Three standard foodstuff plastic packaging namely polyethylene (PE), polypropylene (PP), and polyvinylchloride (PVC) were evaluated for management of lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Resistance parameters in packaging were recorded as punctures, holes, penetrations, sealing defects, and invasions with two thicknesses and tested for two lengths of time. Damages like punctures, holes and penetrations by both insects were more in PE packaging however R. dominica made more penetrations in PP than in PE. For both insects sealing defects and invasions were predominant in PVC than in others. Thickness did not affect significantly damage types but significantly more holes and penetrations by R. dominica were in less thickness. Punctures and holes by R. dominica were more after less time period but other damages in packaging were more after more time period. However for T. castaneum all sorts of damages were seen more after more time period. Overall categorization between two insects showed R. dominica made more penetrations and T. castaneum made more invasions compared with their counterparts. Pictures were taken under camera fitted microscope to magnify punctures and holes in different packaging and thicknesses. Insect mortality due to phosphine was more in PP and PE packaging and least in PVC packaging and thickness effect was marginal. T. castaneum mortality was significantly more after 48 h than after 24 h. Damages extent in packaging and fumigation results showed PP to be the best of three packaging materials to manage these insects. PMID:27638958

  5. Gamma radiation effects of Cobalt-60 on eggs of Tribolium castaneum (Herbst., 1797) (Coleoptera: tenebrionidae); Efeitos da radiacao gama do cobalto-60 em ovos de Tribolium castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae)

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    Fontes, L.S.; Arthur, V. [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil)

    1994-09-01

    The objective of this research was to verify the effects of gamma radiation of a cobalt-60 source on eggs of the red flour beetle Tribolium castaneum (Herbst., 1797). The used dose rate was 1.28 kGy per hour, and the irradiated insects were kept under controlled environmental conditions: 25 {+-} 2{sup 0} C and 70 {+-} 5% relative humidity. (author). 11 refs., 2 figs., 1 tab.

  6. Acute, Lethal and Synergistic Effects of Some Terpenes Against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae

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    M. K. Chaubey

    2012-07-01

    Full Text Available Terpenes are found in abundance in all plant essential oils. In the present study two pure volatile compounds of terpene group, α-pinene and β-caryophyllene have been evaluated for their repellent, acute toxicity and developmental inhibitory activities alone and in binary combination against red flour beetle Tribolium castaneum. In repellency assay, α-pinene and β-caryophyllene repelled T. castaneum adults significantly even at 0.025% concentrations. Fumigation of larvae and adults of T. castaneum with these two compounds caused lethality in them. Median lethal concentrations (LC50 of α-pinene and β-caryophyllene against adults were 0.998 and 1.624 μl/cm3 and against larvae were 1.379 and 1.949 μl/cm3 respectively. In binary combination, the LC50 values against adults and larvae were found 1.277 and 1.438 μl/cm3 respectively. Fumigation with two sublethal concentrations viz. 40 and 80% of 24-h LC50 of these two compunds alone and in binary combination significantly reduced oviposition potential of adults and inhibited pupation and adult emergence in larvae. All the responses were found concentration-dependent. From the present study, it can be concluded that α-pinene is more active than β-caryophyllene and these two volatile compounds in binary combination shows synergism and thus, can used as efficient insecticidal tool against T. castaneum as fumigant either alone or in combination.

  7. FUMIGANT EFFICACY OF 1,8-CINEOLE AND EUGENOL ON THE PUPAL STAGE OF TRIBOLIUM CASTANEUM (HERBST (INSECTA: COLEOPTERA: TENEBRIONIDAE

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    Anita Liška

    2015-12-01

    Full Text Available The fumigant efficacy of 1,8-cineole and eugenol compounds was tested on the pupal stage of the red flour beetle Tribolium castaneum (Herbst. Effects of those compounds were determined as mortality as well as impact on development of treated male and female pupae. Also differences in sensitivity to the tested compounds between sexes of T. castaneum pupae were assessed. Compounds were tested at 3 doses rate 0.34, 0.86 and 1.71 ml L-1 vol. at 30±1°C and 70±10% r.h. during 48 h. Compounds toxicity was expressed in two action modes: lethal, and as an impediment to normal metamorphosis of pupae into adult stage, forming "adultoids" and deformed adults (at both sexes. Male pupae were generally more sensitive to both tested compounds. Overall, better efficiency was performed with 1,8-cineole, while eugenol had not accomplished promising effect on the tested pupae.

  8. Identification, Characterization, and Expression of P450 Gene Encoding CYP6BQ13v2 from the Red Flour Beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

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    XU Yong-qiang; WANG Jin-jun; JIANG Hong-bo; DOU Wei; TANG Pei-an; AN Feng-ming

    2009-01-01

    An allele of CYP6BQ13, named CYP6BQ13v2 (GenBank accession no. FJ209361), was isolated from the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) by RT-PCR. The cDNA sequence of CYP6BQ13v2, 1 563 bp in length, contains an open reading frame of 1 554 nucleotides encoding a putative protein of 518 amino acid residues with a predicted molecular weight of 59.92 kDa and a theoretical pI of 7.60. The putative protein contains the classic berne-binding sequence motif F××G×××C×G (residues 456-465) conserved among all P450 enzymes as well as other characteristic motifs of all cytochrome P450s. It shares 98% identity with the previously published sequence of CYP6BQ13 (GenBank accession no. XP_967146) from the T. castaneum genome project. Phylogenetic analysis of amino acid sequences from members of various P450 families indicated that there was closer phylogenetic relationship of CYP6BQ13v2 with CYP302A1 and CYP307A1 mediating synthesis of the insect molting hormone, distant relationship with CYP6B1 metabolizing plant allelochemicals, CYP6D1 linking to pyrethroid resistance and other members of CYP6 family. Expression test of the gene in the adults and immature stages of T. castaneum by quantitative real-time PCR revealed that CYP6BQ13v2 is expressed in all life stages investigated. The mRNA expression level in 1st instar larvae was 14.9-and 3.86-fold higher than those in pupae and adults, respectively. The CYP6BQ13v2 expression levels appeared in the order of 1st instar larvae, followed by 4th instar larvae, 7th instar larvae, adult, and pupae from high to low. The more bioinformation of CYP6BQ13v2 was also analyzed.

  9. Differential preference of colored surface in Tribolium castaneum (Herbst

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    AMS Reza

    2006-07-01

    Full Text Available Insects show color preferences mostly to those which resembles the color of foliage, flower or even their hosts. In the present study observations were made to determine vision towards different colored surfaces in young (second instar, and advanced (fourth instar larvae, and adults of Tribolium castaneum (Herbst (Coleoptera: Tenebrionidae. The larva and adult beetles showed significant color preferences when given a choice between white (control and colored surfaces at 24- and 48-h exposures. The second instar larvae were more attracted by yellow and pink than green surfaces. The fourth instar larvae did not show any significant preference between white and colored surfaces at 24-h exposure, but avoided red and pink surfaces (P>0.05 and had a marginal choice for black (P<0.05. The adults avoided green significantly at both exposure times and pink at 48-h exposure, but was moderately attracted by black (P<0.05 at both exposure times.

  10. Effects ofArtemisia herba-alba essential oils on survival stored cereal pests:Tribolium castaneum (Herbst) (Coleoptera, Tenebrionidae) and Trogoderma granarium (Everst) (Coleoptera, Dermestidae)

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    Ben Slimane Badreddine; Mariem Baouindi

    2016-01-01

    Objective:To assess the chemical components and toxicity ofArtemisia herba-alba (A. herba-alba) essential oil against two major stored cereal pests,Tribolium castaneum (T. castaneum) andTrogoderma granarium(T. granarium). Methods:Two bioassay actions were tasted: repellent and fumigant actions against adult and larvae, respectively, to assess the effect ofA. herba-alba essential oil. Results: Gas chromatography-mass spectrometer analyses of the essential oil containedβ-thujone (12.50%),α-thujone (8.78%), sabinyl acetate (8.56%), terpinene-4-ol (8.51%),α-terpineol (3.35%), 1,8-cineol (5.45%),γ-terpene (4.82%), camphor (4.52%), dimethyl-ethylbenzene (3.93%) andα-terpinene (3.35%) as the major components. Fumigant toxicity tests showed thatA. herba-alba oil was more toxic thanT. granarium (LC50 = 2.09 mg/mL, LC90 = 4.12 mg/mL) andT. castaneum (LC50 = 6.39 mg/mL, LC90 = 10.10 mg/mL). Conclusions: This study has highlighted a bioinsecticide activity ofA. herba-alba against two insect pests of stored foodstuffs (T. castaneum andT. granarium). TheArtemisia essential oil offers an interesting potential insecticide that could be studied more deeply to isolate and identify the active substances, to study their physiological impact on other insects.

  11. Morpho-functional characterization and esterase patterns of the midgut of Tribolium castaneum Herbst, 1797 (Coleoptera: Tenebrionidae) parasitized by Gregarina cuneata (Apicomplexa: Eugregarinidae).

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    Gigliolli, Adriana A Sinópolis; Lapenta, Ana Silva; Ruvolo-Takasusuki, Maria Claudia Colla; Abrahão, Josielle; Conte, Hélio

    2015-09-01

    Tribolium castaneum (Coleoptera: Tenebrionidae) is a common pest of stored grains and byproducts and is normally infected by Gregarina cuneata (Apicomplexa: Eugregarinidae). The life cycle of this parasite includes the sporozoite, trophozoite, gamont, gametocyte, and oocyst stages, which occur between the epithelium and lumen of the host's midgut. This study aims to describe the morphofunctional alterations in the midgut and determine the esterase patterns in T. castaneum when parasitized by gregarines. To achieve this purpose, midguts of adult insects were isolated, processed, and analysed using light and electron microscopy. We determined total protein content, amylase activity, and the expression and related activities of the esterases by using polyacrylamide gel electrophoresis (PAGE). The midgut of T. castaneum is formed by digestive, regenerative, and endocrine cells. The effects of parasitism on the digestive cells are severe, because the gregarines remain attached to these cells to absorb all the nutrients they need throughout their development. In these cells, the most common alterations observed include expansion and fragmentation of the rough endoplasmic reticulum, development of the smooth endoplasmic reticulum, changes in mitochondrial cristae, cytoplasmic vacuolization, formation of myelin structures, spherites, large intercellular spaces, autophagic vesicles, expansion of the basal labyrinth, and cytoplasmic protrusions. Deposits of glycogen granules were also observed. Amylase activity was reduced in parasitized insects. Regenerative cells were found in disorganized crypts and did not differentiate into new cells, thus, compromising the restoration of the damaged epithelium. Though few morphological alterations were observed in the endocrine cells, results suggest that the synthesis and/or release of hormones might be impaired. Nine esterases (EST-1 to 9) were identified in the midgut of T. castaneum and were expressed in varying levels in response

  12. Evaluación de la actividad repelente e insecticida de aceites esenciales extraídos de plantas aromáticas utilizados contra tribolium castaneum herbst (coleoptera: tenebrionidae)

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    Espitia Yanes, Carmen Regina

    2011-01-01

    Los aceites esenciales son mezclas volátiles de terpenos obtenidas de plantas. Estos productos poseen propiedades repelentes e insecticida de insectos. Uno de los insectos de mayor importancia económica es el Tribolium castaneum Herbst. Con el objetivo de evaluar la actividad de aceites esenciales de plantas de Colombia Cymbopogon citratus y (GRbgV02E), Lippia origanoides (VEbgV07E) y Eucalyptus citriodora (MYbgV01E) fueron realizados ensayos de repelencia y mortalidad. Para tal efecto, fué u...

  13. Pesticidal Evaluation of Manilkara zapota (L. against Tribolium castaneum (Herbst

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    Muhammad Abu Osman

    2014-02-01

    Full Text Available This paper presents the residual eff ects of Manilkara zapota (L. P. Royen plant against the red flour beetle, Tribolium castaneum (Herbst. Four doses, i.e., 1238.5, 619.25, 309.6 and 154.8 μg/cm2 of ethyl acetate extract of stem bark of Manilkara zapota were applied on larvae and adult beetles. The effectiveness of the plant extract was increased with the increase of exposure time and after 72 hrs of exposure, the maximum residual toxicity was observed with LD50 of 228.8, 281.1, 413.4, 423.7, 455.2, 498.7 and 526.5 μg/cm2 for first, second, third, fourth, fifth, sixth instar larvae and adults, respectively. The results of this study also demonstrated that the earlier instars were more sensitive to the extracts than those of late instars as well as adults.

  14. Pesticidal Evaluation of Manilkara zapota (L. against Tribolium castaneum (Herbst

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    Muhammad Abu Osman

    2011-06-01

    Full Text Available This paper presents the residual eff ects of Manilkara zapota (L. P. Royen plant against the red flour beetle, Tribolium castaneum (Herbst. Four doses, i.e., 1238.5, 619.25, 309.6 and 154.8 μg/cm2 of ethyl acetate extract of stem bark of Manilkara zapota were applied on larvae and adult beetles. The effectiveness of the plant extract was increased with the increase of exposure time and after 72 hrs of exposure, the maximum residual toxicity was observed with LD50 of 228.8, 281.1, 413.4, 423.7, 455.2, 498.7 and 526.5 μg/cm2 for first, second, third, fourth, fifth, sixth instar larvae and adults, respectively. The results of this study also demonstrated that the earlier instars were more sensitive to the extracts than those of late instars as well as adults.

  15. 七种糕点对赤拟谷盗(鞘翅目:拟步甲科)感染的抗性%Resistance of Seven Biscuit Types to Infestation by Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

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    Olusola O. ODEYEMI; Bernice M. OYEDARE; Michael O. ASHAMO

    2005-01-01

    Seven biscuit types namely, Okin, Digestive, Cabin, Peanut, Cream crackers, Hobnobs and Glucose and wheat flour were screened for their resistance to Tribolium castaneum (Herbst) at ambient temperature of 28±2 ℃ and 78±2% relative humidity in the laboratory. 50 g of each biscuit sample were infested with four female and two male adult beetles and left for 15 days then remove for a resistant experiment and 70 days for a mortality experiment. Each treatment and the control without beetles were replicated three times. Results showed that there was significant difference (P0.05) in the weight loss of biscuit types. The highest adult mortality of beetles (100%) was obtained from Peanut biscuits at 28 days after infestation while there was only 5.5% adult mortality in wheat flour at 70 days after infestation. The susceptibility index was 0 for all the biscuit types since there was no adult emergence indicating that they were resistant to T. castaneum infestation. Resistance in the biscuit types could be due to chemical additives (e.g. sodium chloride and sodium bicarbonate) used in the production of biscuits, which may have inhibitory effect on the development of T. castaneum and also the low moisture content of the biscuits. Packages that can easily be perforated or damaged to allow absorption of moisture from the environment should not be used in packing biscuits.

  16. NSECTICIDAL TOXICITY OF 1,8-CINEOLE, CAMPHOR AND EUGENOL ON TRIBOLIUM CASTANEUM (HERBST)

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    Anita Liška

    2011-01-01

    Contact and fumigant activity of 1,8-cineole, camphor and eugenol compounds were tested In laboratory conditions on adult, larvae and pupae of the red flour beetle Tribolium castaneum (Herbst), as well as their effect on progeny emergence. Positive results were obtained with contact application for all three tested compounds and on all three stages of development of T. castaneum, with the highest activity of 1,8-cineole, followed by eugenol and camphor. In general, fumigant activity of all th...

  17. INSECTICIDAL TOXICITY OF 1,8-CINEOLE, CAMPHOR AND EUGENOL ON TRIBOLIUM CASTANEUM (HERBST)

    OpenAIRE

    Anita Liška

    2011-01-01

    Contact and fumigant activity of 1,8-cineole, camphor and eugenol compounds were tested In laboratory conditions on adult, larvae and pupae of the red flour beetle Tribolium castaneum (Herbst), as well as their effect on progeny emergence. Positive results were obtained with contact application for all three tested compounds and on all three stages of development of T. castaneum, with the highest activity of 1,8-cineole, followed by eugenol and camphor. Ingeneral, fumi...

  18. Effects of methoprene and synergized pyrethrin aerosol applications on Tribolium castaneum (Herbst) populations

    Science.gov (United States)

    Experiments were performed to investigate the effects of horizontal transfer of the insect growth regulator (IGR) methoprene on confined populations of Tribolium castaneum (Herbst) either with or without hidden refugia. Multiple applications were made with the IGR alone or combined with synergized p...

  19. Mechanisms for horizontal transfer of methoprene from treated to untreated Tribolium castaneum (Herbst)

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    Experiments were performed to determine the relative impact of different mechanisms of horizontal transfer of methoprene by Tribolium castaneum (Herbst), the red flour beetle. Insects exposed to 5 methoprene treated developmental stages (late-stage larvae, pupae, or adults) resulted in 100% mortalit...

  20. Toxicity of Boldo Peumus boldus Molina for Sitophilus zeamais Motschulsky and Tribolium castaneum Herbst Toxicidad del Boldo, Peumus boldus Molina, sobre Sitophilus zeamais Motschulsky y Tribolium castaneum Herbst

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    Margarita Ortiz U

    2012-09-01

    Full Text Available The maize weevil (Sitophilus zeamais Motschulsky and the red flour beetle (Tribolium castaneum Herbst are two key pests of stored-grain products worldwide. The insecticidal activity of boldo (Peumus boldus Molina powder, liquid ethanolic and hexanic extracts against S. zeamais and T. castaneum were evaluated under laboratory conditions. The evaluated variables were mortality, emergence of adult insects (F1, and grain weight loss. The experimental design was completely randomized. The mortality in S. zeamais was 100% even at the lowest powder concentration (0.5% w/w, whereas emergence of F1 adult insects was 0% and grain weight loss was El gorgojo del maíz (Sitophilus zeamais Motschulsky y el gorgojo castaño de la harina (Tribolium castaneum Herbst son plagas primarias de productos almacenados a nivel mundial. Se evaluó en laboratorio la actividad insecticida de polvo y extractos líquidos etanólicos y hexánicos del boldo (Peumus boldus Molina sobre S. zeamais y T. castaneum. Las variables evaluadas fueron mortalidad y emergencia de insectos adultos (F1 y pérdida de peso de los granos con un diseño experimental completamente al azar. La mortalidad en S. zeamais fue 100%, incluso con la concentración menor (0,5% p/p mientras que la emergencia de insectos adultos y la pérdida de peso de granos de maíz fue < 0,08%. Para T. castaneum sólo las concentraciones de 8 y 16% p/p de polvo causaron una mortalidad de 100%. Los extractos en agua, etanol, y hexano tuvieron un efecto insecticida de 100% en S. zeamais, mientras que en T. castaneum sólo el extracto en etanol alcanzó este valor. Por lo tanto, el polvo y los extractos evaluados de P. boldus presentan actividad insecticida contra S. zeamais y T. castaneum y son promisorios para utilizarse contra éstas y otras plagas de granos almacenados.

  1. Laboratory evaluation of some indigenous plant extracts as toxicants against red flour beetle, Tribolium castaneum Herbst

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    Mamum, M.S.A; Shahjahan, M.; Ahmad, M.

    2009-01-01

    Experiments were carried out to evaluate the toxicity of six botanicals, Bazna (Zanthoxylum rhetsa), Ghora-neem (Melia sempervirens), Hijal (Barringtonia acutangula), Karanja (Pongamia pinnata), Mahogoni (Swietenia mahagoni) and Neem (Azadirachta indica) against red flour beetle, Tribolium castaneum Herbst. Leaf and seed extracts were prepared by using acetone, methanol and water as solvents. The results showed that extracts of all the six plants had direct toxic effect on red flour beetle. A...

  2. BIOACTIVITY OF 1,8-CINEOLE AGAINST RED FLOUR BEETLE TRIBOLIUM CASTANEUM (HERBST

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    Anita Liška

    2011-06-01

    Full Text Available Red flour beetle Tribolium castaneum (Herbst is a major pest of stored products. The aim of this study was to assess the potential fumigant effects of 1,8-cineole, essential oil component, on the T. castaneum pupae. The compound was tested in 6 doses; in two treatments (fumigation without grain and with wheat grain, exposed for 48 h, in 4 repetitions, for each gender. The compound 1,8-cineole had lethal effect on the treated pupae at both genders and in the both treatments. Total proportion of the normally developed beetles was decreased. In addition, 1,8-cineole had also a growth regulator effect, producing adultoids and deformed units, with males more susceptible. In the treatment with the grain there were significant lower dead pupae, normally developed live male beetles and also deformed female units in the stage 2. In general, compound 1,8-cineole has multiple effect against T. castaneum in pupal stage.

  3. Effect of gamma radiation on the eggs of Tribolium castaneum (Herbst)

    Energy Technology Data Exchange (ETDEWEB)

    Mehta, V.K.; Sethi, G.R. (Indian Agricultural Research Inst., New Delhi. Nuclear Research Lab.)

    1984-09-01

    Studies on the susceptibility of eggs of Tribolium castaneum (Herbst) to gamma radiation revealed that the radiosensitivity of eggs decreased with the increasing age of the embryo and the development of the embryo was arrested if the eggs were irradiated in the early stage of development (24-48 hr old). However, when 72-96 hr old eggs were irradiated, comparatively higher dose was required to render them nonviable. Mortality of the eggs of the same age group was directly related to the radiation dose. Survival of the insect to the adult stage was prevented by exposure to a dose of 8 Krad or more, irrespective of the age of the eggs.

  4. Capture of Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) in Floor Traps: The Effect of Previous Captures.

    Science.gov (United States)

    Athanassiou, Christos G; Kavallieratos, Nickolas G; Campbell, James F

    2016-02-01

    The impact of prior captures on the trapping performance of floor traps was evaluated for the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), and the confused flour beetle, Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae), in laboratory conditions. The effect of trap seeding, adding adults of the same or different species, was evaluated in order to determine possible effects of prior captures in the trap on each species' behavioral responses. The presence of seeded beetles of the same species resulted in an increase in beetle captures for both T. castaneum and T. confusum, but when traps were seeded with the opposite species, there was no increase in beetle captures for either species, and for T. castaneum overall captures in both seeded and unseeded traps was reduced. Overall, T. castaneum tended to have greater captures than T. confusum regardless of the treatment. When the two species were released together, this negated the increased response to seeded traps observed in the single-species treatments. These findings suggest the potential that the presence of beetles in a trap may be influencing the response of beetles in a nearby trap and that T. castaneum and T. confusum when they occur together may influence each other's response to traps.

  5. Susceptibility of Tribolium castaneum (Coleoptera: Tenebrionidae) and Trogoderma inclusum (Coleoptera: Dermestidae) to cold temperatures

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    Studies were conducted by exposing different life stages of Tribolium castaneum (Herbst), the red flour beetle, and Trogoderma variabile (Ballion), the warehouse beetle, for different time intervals to -18°C. Assessments were made of direct mortality to eggs, larvae, and adults, and eventual adult e...

  6. Efficacies of spinosad and a combination of chlorpyrifos-methyl and deltamethrin against phosphine-resistant Rhyzopertha dominica (Coleoptera: Bostrichidae) and Tribolium castaneum (Coleoptera: Tenebrionidae) on wheat.

    Science.gov (United States)

    Bajracharya, N S; Opit, George P; Talley, J; Jones, C L

    2013-10-01

    Highly phosphine-resistant populations of Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) have recently been found in Oklahoma grain storage facilities. These findings necessitate development of a phosphine resistance management strategy to ensure continued effective use of phosphine. Therefore, we investigated the efficacies of two grain insecticides, namely, spinosad applied at label rate of 1 ppm and a mixture of chlorpyrifos-methyl and deltamethrin applied at label rates of 3 and 0.5 ppm, respectively, against highly phosphine-resistant R. dominica and T. castaneum. Adult mortality and progeny production suppression of spinosad- or chlorpyrifos-methyl + deltamethrin mixture-treated wheat that had been stored for 2, 84, 168, 252, and 336 d posttreatment were assessed. We found that both spinosad and chlorpyrifos-methyl + deltamethrin were effective against phosphine-resistant R. dominica and caused 83-100% mortality and also caused total progeny production suppression for all storage periods. Spinosad was not effective against phosphine-resistant T. castaneum; the highest mortality observed was only 3% for all the storage periods. Chlorpyrifos-methyl + deltamethrin was effective against phosphine-resistant T. castaneum only in treated wheat stored for 2 and 84 d, where it caused 93-99% mortality. However, chlorpyrifos-methyl + deltamethrin was effective and achieved total suppression of progeny production in T. castaneum for all the storage periods. Spinosad was not as effective as chlorpyrifos-methyl + deltamethrin mixture at suppressing progeny production of phosphine-resistant T. castaneum. These two insecticides can be used in a phosphine resistance management strategy for R. dominica and T. castaneum in the United States.

  7. EFFECTS OF ESSENTIAL OIL FORMULATIONS ON THE ADULT INSECT TRIBOLIUM CASTANEUM (HERBST (COL., TENEBRIONIDAE

    Directory of Open Access Journals (Sweden)

    Aleksandra Popovic

    2013-06-01

    Full Text Available Stored product pests such as Tribolium castaneum ( Herbst, 1979 are a major problem. Adult insects were obtained from laboratory cultures maintained in the dark in incubators at 25 1C and 70 80percent r.h., reared on wheat flour and fed with flour disks containing a known concentration of essential oil of 9 plants. The chemical components of essential oil of 3 plants, collected on the area of Montenegro, were also identified using GC-MS analysis. The results of insecticidal effect of essential oils were discussed. Also, mortality rate of adult insects was tested. In this research, the essential oils of C. glandulosa which were rich in monoterpene alcohols carvacrol and contained ketonic component showed strong insecticidal and fumigant activity against adults of T. castaneum. Less toxic effect showed essential oils of Satureja montana which had a lower carvacrol and ketonic content. On the other hand, essential oils of Teucrium polium which did not contain ketonic component did not show any activity. Therefore, it was observed that essential oils of C. glandulosa with concentration of 1.14% showed powerful toxic and repellent effect, with very high mortality rate after 24h (56,67%.

  8. Contact toxicity of deltamethrin against Tribolium castaneum (Coleoptera: Tenebrionidae), Sitophilus oryzae (Coleoptera: Curculionidae), and Rhyzopertha dominica (Coleoptera: Bostrichidae) adults

    Science.gov (United States)

    This study was conducted to evaluate deltamethrin-incorporated ZeroFly® Storage Bags for efficacy against stored-product insect pests. We evaluated response to deltamethrin concentrations for adults of three stored-product insects, Tribolium castaneum (Herbst), Sitophilus oryzae (L.), and Rhyzoperth...

  9. Toxicity of several contact insecticides to Tribolium castaneum (Herbst) populations after selection with pirimiphos-methyl and deltamethrin

    OpenAIRE

    Andrić Goran; Pražić-Golić Marijana; Kljajić Petar

    2015-01-01

    Laboratory bioassays were conducted to detect possible alteration in susceptibility of two field Tribolium castaneum (Herbst) populations (sampled in a warehouse in Nikinci and a silo in Jakovo) to dichlorvos, malathion, chlorpyrifos-methyl, pirimiphos-methyl, deltamethrin and bifenthrin after previous selection with the LD80 of pirimiphos-methyl and deltamethrin. Data from the topical application bioassays show that chlorpyrifos-methyl was the most toxic i...

  10. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae. A atividade inseticida de Toona sureni (Blume Merr. foi avaliada considerando repelência, mortalidade e a produção de progênie de adultos F1 de Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Extrato seco de sementes

  11. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.

  12. Life History of the Tamarind Weevil, Sitophilus linearis (Herbst) (Coleoptera: Curculionidae), on Tamarind Seed

    OpenAIRE

    James Adebayo Ojo; Adebayo Amos Omoloye

    2015-01-01

    The tamarind weevil, Sitophilus linearis Herbst (Coleoptera: Curculionidae), is an important pest of tamarind and other Caesalpinioideae. Investigating its life history is important in the implementation of management strategy. Its life history was monitored daily to understand its developmental biology on tamarind seed following standard procedures under laboratory conditions of 24–30°C temperature, 60–70% relative humidity, and 12L : 12D photoperiod. The egg incubation period lasted 3.17 ± ...

  13. Age and sex related responsiveness of Tribolium castaneum (Coleoptera: Tenebrionidae) in novel behavioral bioassays

    Science.gov (United States)

    The hardiness and mobile nature of Tribolium castaneum (Herbst) make them easy to work with but are the same factors that make their responses to behavior modifying chemical stimuli difficult to evaluate. To overcome these difficulties we developed two bioassays: a two choice test with airflow and a...

  14. A family of chemoreceptors in Tribolium castaneum (Tenebrionidae: Coleoptera.

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    Mohatmed Abdel-Latief

    Full Text Available Chemoperception in invertebrates is mediated by a family of G-protein-coupled receptors (GPCR. To date nothing is known about the molecular mechanisms of chemoperception in coleopteran species. Recently the genome of Tribolium castaneum was sequenced for use as a model species for the Coleoptera. Using blast searches analyses of the T. castaneum genome with previously predicted amino acid sequences of insect chemoreceptor genes, a putative chemoreceptor family consisting of 62 gustatory receptors (Grs and 26 olfactory receptors (Ors was identified. The receptors have seven transmembrane domains (7TMs and all belong to the GPCR receptor family. The expression of the T. castaneum chemoreceptor genes was investigated using quantification real- time RT-PCR and in situ whole mount RT-PCR analysis in the antennae, mouth parts, and prolegs of the adults and larvae. All of the predicted TcasGrs were expressed in the labium, maxillae, and prolegs of the adults but TcasGr13, 19, 28, 47, 62, 98, and 61 were not expressed in the prolegs. The TcasOrs were localized only in the antennae and not in any of the beetles gustatory organs with one exception; the TcasOr16 (like DmelOr83b, which was localized in the antennae, labium, and prolegs of the beetles. A group of six TcasGrs that presents a lineage with the sugar receptors subfamily in Drosophila melanogaster were localized in the lacinia of the Tribolium larvae. TcasGr1, 3, and 39, presented an ortholog to CO(2 receptors in D. melanogaster and Anopheles gambiae was recorded. Low expression of almost all of the predicted chemoreceptor genes was observed in the head tissues that contain the brains and suboesophageal ganglion (SOG. These findings demonstrate the identification of a chemoreceptor family in Tribolium, which is evolutionarily related to other insect species.

  15. Evaluation of Reference Genes for RT-qPCR in Tribolium castaneum (Coleoptera: Tenebrionidae) Under UVB Stress.

    Science.gov (United States)

    Sang, Wen; He, Li; Wang, Xiao-Ping; Zhu-Salzman, Keyan; Lei, Chao-Liang

    2015-04-01

    Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) has become a widely used technique to quantify gene expression. It is necessary to select appropriate reference genes for normalization. In the present study, we assessed the expression stability of seven candidate genes in Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) irradiated by ultraviolet B (UVB) at different developmental stages for various irradiation time periods. The algorithms of geNorm, NormFinder, and BestKeeper were applied to determine the stability of these candidate genes. Ribosomal protein genes RpS3, RpL13A, and β-actin gene (ActB) showed the highest stability across all UVB irradiation time points, whereas expression of other normally used reference genes, such as those encoding the β-tubulin gene TUBB and the E-cadherin gene CAD, varied at different developmental stages. This study will potentially provide more suitable reference gene candidates for RT-qPCR analysis in T. castaneum subjected to environmental stresses, particularly UV irradiation.

  16. Toxicity of several contact insecticides to Tribolium castaneum (Herbst populations after selection with pirimiphos-methyl and deltamethrin

    Directory of Open Access Journals (Sweden)

    Goran Andrić

    2015-12-01

    Full Text Available Laboratory bioassays were conducted to detect possible alteration in susceptibility of two field Tribolium castaneum (Herbst populations (sampled in a warehouse in Nikinci and a silo in Jakovo to dichlorvos, malathion, chlorpyrifos-methyl, pirimiphos-methyl, deltamethrin and bifenthrin after previous selection with the LD80 of pirimiphos-methyl and deltamethrin. Data from the topical application bioassays show that chlorpyrifos-methyl was the most toxic insecticide to T. castaneum adults of the Nikinci population selected with pirimiphosmethyl and deltamethrin, while malathion was the weakest, and both selection procedures changed/reduced significantly only the toxicity of deltamethrin and bifenthrin, increasing their resistance ratios (RR at the LD50 from 1.1 to 1.8 (bifenthrin and from 0.9 to 2.2 (deltamethrin. Deltamethrin was the most toxic insecticide for Jakovo adults selected with the LD80 of pirimiphosmethyl, while malathion was again the least toxic. Selection of that population had no effect on insecticide toxicity, except of malathion, which had a rise in RR at the LD50 from 26.0 to 29.8.

  17. Insecticidal activity of Ageratum conyzoides L., Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit essential oils as fumigant against storage grain insect Tribolium castaneum Herbst

    OpenAIRE

    Jaya; Singh, Priyanka; Prakash, Bhanu; Dubey, N.K.

    2012-01-01

    Essential oils (EOs) from Ageratum conyzoides L., Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit were extracted and tested against Tribolium castaneum Herbst, the storage grain insect. The EOs were found effective against Tribolium castaneum during in vitro as well as in vivo fumigant testing. The EOs of H. suaveolens and A. conyzoides showed 100 % mortality of test insect at 250 ppm while C. aromaticus at 350 ppm. During in vivo fumigant testing of wheat samples against Tribolium c...

  18. Repellent activity of essential oils and some of their individual constituents against Tribolium castaneum herbst.

    Science.gov (United States)

    Caballero-Gallardo, Karina; Olivero-Verbel, Jesús; Stashenko, Elena E

    2011-03-01

    A tool for integrated pest management is the use of essential oils (EOs) and plant extracts. In this study, EOs from Tagetes lucida , Lepechinia betonicifolia , Lippia alba , Cananga odorata , and Rosmarinus officinalis , species grown in Colombia, were analyzed by gas chromatography-mass spectrometry. These oils as well as several of their constituents were tested for repellent activity against Tribolium castaneum , using the area preference method. The main components (>10%) found in EOs were methylchavicol, limonene/α-pinene, carvone/limonene, benzyl acetate/linalool/benzyl benzoate, and α-pinene, for T. lucida, L. betonicifolia, L. alba, C. odorata, and R. officinalis, respectively. All EOs were repellent, followed a dose-response relationship, and had bioactivity similar to or better than that of commercial compound IR3535. EOs from C. odorata and L. alba were the most active. Compounds from EOs, such benzyl benzoate, β-myrcene, and carvone, showed good repellent properties. In short, EOs from plants cultivated in Colombia are sources of repellents against T. castaneum. PMID:21291237

  19. Life History of the Tamarind Weevil, Sitophilus linearis (Herbst (Coleoptera: Curculionidae, on Tamarind Seed

    Directory of Open Access Journals (Sweden)

    James Adebayo Ojo

    2015-01-01

    Full Text Available The tamarind weevil, Sitophilus linearis Herbst (Coleoptera: Curculionidae, is an important pest of tamarind and other Caesalpinioideae. Investigating its life history is important in the implementation of management strategy. Its life history was monitored daily to understand its developmental biology on tamarind seed following standard procedures under laboratory conditions of 24–30°C temperature, 60–70% relative humidity, and 12L : 12D photoperiod. The egg incubation period lasted 3.17 ± 0.07 days. A mated female of S. linearis laid an average of 165 ± 5.78 eggs during an oviposition period of 86.8 ± 2.47 days. There were four larval instars, with a total larval developmental period of 16 days. The pupal period lasted 8 days, and adult lived 108.5 ± 3.61 days. The overall growth ratio for the four instars was 1.33. There was a regular relationship and significant correlation (r=0.94 between the stages of larval development and head capsule width.

  20. Actividad insecticida del aceite esencial de Lippia alba (Mill.) N. E. Brown sobre Tribolium castaneum Herbst. en granos de trigo (Triticum aestivum L.)

    OpenAIRE

    Ringuelet, Jorge Abel; Ocampo, Rafael; Henning, Cynthia; Padín, Susana; Urrutia, María Inés; Dal Bello, Gustavo

    2014-01-01

    Tribolium castaneum (Coleoptera: Tenebrionidae) es un insecto plaga de granos, muy destructivo en la etapa de almacenamiento y altamente resistente a los fitosanitarios sintéticos. Por esta razón su control químico se realiza con altas concentraciones de insecticidas que aumentan la contaminación ambiental y los peligros para la salud humana. Las tendências actuales en el manejo integrado de plagas se orientan hacia el uso de plaguicidas biológicos o biopesticidas como los extractos vegetales...

  1. Impact of varying levels of sanitation on mortality of Tribolium castaneum eggs and adults during heat treatment of a pilot flour mill

    Science.gov (United States)

    The influence of sanitation on responses of life stages of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), an economically important pest in flour mills, was investigated in a pilot flour mill subjected to two, 24-h heat treatments. One hundred eggs or 100 adults of T...

  2. Evaluación de la acción insecticida de la rapanona sobre Tribolium castaneum (Herbst

    Directory of Open Access Journals (Sweden)

    Chavez Germán

    1992-11-01

    Full Text Available Experiments were carried out to detect and estimate the insect-killing effect of rapanone on the flour beetle  T.castaneum. Two kinds ofessays were made: by ingestion and by contacto It was proved that rapanone has insecticide activity on T. castaneum. Relatively high LD50 were determined, in comprarison
    with those of conventional commercial insecticides. An inverse proportionality, between doses of rapanone and the mean productivity, was also found; adose of 7000 mg rapanone per kg standard culture medium is enough to exterminate, almost completely, a population of T. castaneum in one generation.
    Se realizaron experimentos para detectar y cuantificar efectos insecticidas de la rapa nona sobre el escarabajo de la harina, Tribolium castaneum. Las pruebas fueron de dos clases: por ingestión y por contacto. Se comprobó que la rapanona tiene propiedades insecticidas sobre T. castaneum. Las toxicidades agudas, medidas en términos de DL50 fueron relativamente altas comparadas con insecticidas sintéticos comerciales. También se encontró una proporcionalidad inversa entre dosis de rapanona y productividad promedio; una dosis de 7000 mg de rapanona por kilo de alimento, es suficiente para exterminar casi del todo una poblaci6n de T.castaneum en una sola generación.

  3. Models to predict mortality of Tribolium castaneum (Coleoptera: Tenebrionidae) exposed to elevated temperatures during structural heat treatments.

    Science.gov (United States)

    Jian, Fuji; Subramanyam, Bhadriraju; Jayas, Digvir S; White, Noel D G

    2013-10-01

    Novel thermal death models were developed with certain assumptions, and these models were validated by using actual heat treatment data collected under laboratory conditions at constant temperatures over time and in commercial food-processing facilities where temperatures were dynamically changing over time. The predicted mortalities of both young larvae and adults of the red flour beetle, Tribolium castaneum (Herbst), were within 92-99% of actual measured insect mortalities. There was good concordance between predicted and observed mortalities of young larvae and adults of T. castaneum exposed to constant temperatures in laboratory growth chambers and at variable temperatures during structural heat treatments of commercial food-processing facilities. The models developed in this study can be used to determine effectiveness of structural heat treatments in killing young larvae and adults of T. castaneum and for characterizing insect thermotolerance.

  4. Transovarial Effect of Novaluron on Tribolium castaneum (Coleoptera: Tenebrionidae) After Termination of Direct Contact.

    Science.gov (United States)

    Trostanetsky, A; Kostyukovsky, M; Quinn, E

    2015-01-01

    The insect growth regulator novaluron (Rimon 10 EC, Makhteshim-Agan Ltd, Israel) is used against many field pests on corn, vegetables, orchards, forests, and cotton plantations. Previously, we studied various effects of novaluron on stored grain pests. Termination in Tribolium castaneum (Herbst) eggs hatching after treating adults with novaluron and following restoration after adult transfer to untreated media was observed. The objective of this study was to investigate the restoration of T. castaneum egg hatch following transfer of adults from treated media to untreated favorable and unfavorable media. The time needed for hatching restoration of 50% of eggs laid by adults transferred from novaluron (1 ppm) treated flour to untreated flour (RT50) was 2.7 d. RT50 for those transferred to untreated wheat grain was 4.1 d. RT90 in flour was 3.6 d, in grain--6.1 d. Varieties of RTs in grain and in flour with nonoverlapping confidence intervals indicate that RTs were significantly different. Delay of eggs hatching restoration for adults transferred from treated flour to unfavorable media (Petri dishes with limited amount of flour, lying of eggs not detected) was observed. RT50 in flour was 2.1 d and RT90--3.1 d, while RT50 in the unfavorable media was 3.4 d and RT90 6.5 d. Delayed effect of egg hatching restoration after adult transfer to unfavorable media provides evidence of the significant role of insect physiological state in novaluron excretion and (or) degradation by T. castaneum females.

  5. Residual efficacy of methoprene for control of Tribolium castaneum (Coleoptera: Tenebrionidae) larvae at different temperatures on varnished wood, concrete, and wheat.

    Science.gov (United States)

    Wijayaratne, L K Wolly; Fields, Paul G; Arthur, Frank H

    2012-04-01

    The residual efficacy of the juvenile hormone analog methoprene (Diacon II) was evaluated in bioassays using larvae of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) exposed on unsealed concrete or varnished wood treated with a liquid formulation and held at different temperatures. When these two types of surfaces were stored at 20, 30 or 35 degrees C for 0-24 wk, the percentage of adult emergence on concrete increased with time. In contrast, there was no adult emergence from larvae exposed to varnished wood at 24 wk after treatment at any of these temperatures. The presence of flour reduced residual efficacy of methoprene on concrete, but not on varnished wood, with no differences between cleaning frequencies. Methoprene was also stable for 48 h on concrete held at 65 degrees C and wheat, Triticum aestivum L., held at 46 degrees C. Results show that methoprene is stable at a range of temperatures commonly encountered in indoor food storage facilities and at high temperatures attained during insecticidal heat treatments of structures. The residual persistence of methoprene applied to different surface substrates may be affected more by the substrate than by temperature.

  6. Insecticidal properties of essential oils against Tribolium castaneum (Herbst) and their inhibitory effects on acetylcholinesterase and adenosine triphosphatases.

    Science.gov (United States)

    Abou-Taleb, Hamdy K; Mohamed, Magdy I E; Shawir, Mohamed S; Abdelgaleil, Samir A M

    2016-01-01

    Essential oils from 20 Egyptian plants were obtained by using hydrodistillation. The chemical composition of the isolated oils was identified by gas chromatograph/mass spectrometer. Fumigant and contact toxicities of the essential oils were evaluated against the adults of Tribolium castaneum. In fumigation assays, the oil of Origanum vulgare (LC50 = 9.97 mg/L air) displayed the highest toxicity towards the adults of T. castaneum. In contact assays, the oils of Artemisia monosperma (LC50 = 0.07 mg/cm(2)) and O. vulgare (LC50 = 0.07 mg/cm(2)) were the most potent toxicants against the adults of T. castaneum. Biochemical studies showed that the tested oils caused pronounced inhibition of acetylcholinesterase (AChE) and adenosine triphosphatases (ATPases) isolated from the larvae of T. castaneum. The oil Cupressus macrocarpa (IC50 = 12.3 mg/L) was the most potent inhibitor of AChE, while the oil of Calistemon viminals (IC50 = 4.4 mg/L) was the most potent inhibitor of ATPases.

  7. Effect of five diatomaceous earth formulations against Tribolium castaneum (Coleoptera: Tenebrionidae),Oryzaephilus surinamensis (Coleoptera: Silvanidae) and Rhyzopertha dominica (Coleoptera: Bostrychidae)

    Institute of Scientific and Technical Information of China (English)

    MASUMEH ZIAEE; ADEL KHASHAVEH

    2007-01-01

    Laboratory bioassays were conducted to determine the effect of food source on the survival of Tribolium castaneum Herbst, Oryzaephilus surinamensis L. and Rhyzopertha dominica F., after exposure to five diatomaceous earth (DE) formulations: Protect-It(R),Insecto(R), Perma-GuardTM, Dryacide(R) and SilicoSec(R). Adults of these species were exposed to DEs at the rate of 0.5 mg/cm2 for 1 day on filter paper inside plastic Petri dishes. After exposure,the initial mortality was counted and live individuals of the three species were held for a week in glass vials containing 50 mg wheat flour, rice and whole wheat, respectively. In the second experiment, after 1 day exposure to DEs, beetles were transferred to Petri dishes without food and held for a week to determine if the presence of food source would decrease the mortality of beetles. Experiments were carried out at 27℃ and 55% RH in the dark. The initial morality in both of the experiments reached 100% for the three species exposed to Protect-It(R) and in the case orR. dominica and O. surinamensis exposed to Dryacide(R). In contrast, low level of mortality (< 10%) was observed for T. castaneum exposed to Perma-GuardTM and Insecto(R). The mortality after the post-treatment period on food was decreased for the three species exposed to Perma-GuardTM and in the case of T. castaneum and R. dominica exposed to Insecto(R) and SilicoSec(R). Adults of O. surinamensis were the most susceptible followed by R. dominica and 100% adult mortality was obtained, whereas T. castaneum were the least susceptible beetles to DEs. Protect-It(R) and Dryacide(R) were the most efficient DE formulations and can be used effectively in a stored grain integrated pest management program.

  8. Effect of salt stressed wheat varieties on life history of tribolium castaneum (hebrst) (tenebrionidae: coleoptera)

    International Nuclear Information System (INIS)

    In the present investigation an attempt was made to determine the antibiosis of three salt stressed wheat varieties (Shafaq, Inqlab-91 and Sehar-2006) at three salinity levels (8, 12 and 16 dS m/sup -1/) to red flour beetle, Tribolium castaneum (Herbst). The grains of three varieties were ground and sieved through 80 mesh. Life history parameters, i.e., larval and pupal durations and survivals, adult emergence, fecundity and egg hatching was observed by introducing 10 pairs of pupae, from previously reared beetles, on three varieties at their respective salt levels. One control was included with grains of the plants, which were irrigated with distilled water only. Results have shown that varieties had significant difference among the treatments for larval duration, which was significantly extended in different varieties at various salt levels. Mean longest larval duration (31.67 days) was recorded in Shafaq as respectively 8 (33.00 days), 12 (33.33 days) and 16 (30.33 days) dS m/sup -1/ salt level as compared to its control (31.00 days). Fecundity and egg hatching of T. castaneum differed among the wheat varieties irrespective of salt levels. In another experiment, response of SARC-1, SARC-2, SARC-3, SARC-4, SARC-5, SARC-6, SARC-7, SARC-8, LU-26S, to life history parameters of T. castaneum showed that significant difference in the number of eggs, hatching percentage, larval and pupal survival was found. The number of eggs was significantly lower in variety SARC 1 (126.00) followed by SARC 2 (128.75), SARC 3 (132.25) while was significantly higher in SARC 5 (151.75). Egg hatching percentage was lower in SARC 6 (39.38%) and significantly higher in SARC 5 (58.42%). Larval survival was significantly less in SARC 7 (36.99%) and more in SAR (52.25%). Pupal survival was significantly lower in SARC 1 (20.54%) while higher in SARC 8 (42.11%). Based on results it may be stated that salt stressed wheat varieties have significant impact of the biology of T. castaneum. (author)

  9. LETHAL EFFECT OF MICROWAVE TREATMENT ON Tribolium castaneum(Herbst)%微波处理对赤拟谷盗各虫态的致死效果

    Institute of Scientific and Technical Information of China (English)

    王殿轩; 刘炎; 曹阳; 李淑荣

    2010-01-01

    分别采用微波功率160、320、480、640和800 W处理裸露的和混合于小麦粉中的各虫态赤拟谷盗Tribolium castaneum(Herbst)的卵、幼虫、蛹和成虫.结果表明:对于裸露的赤拟谷盗用上述功率各处理5、10、15、20、25和30 s时,800 W、25 s可完全致死卵,320 W、30 s可使幼虫和成虫完全致死;800 W、20s可完全致死幼虫、蛹和成虫.对混合于小麦粉中的各虫态赤拟谷盗,当用上述功率各处理20 s时,均不能完全致死各虫态;单独使用800 W分别处理5、10、15、20和25 s时,仅800 W、25 s可完全致死各虫态.微波处理对赤拟谷盗杀虫效果显著.

  10. Evaluation of structural treatment efficacy against Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) using meta-analysis of multiple studies conducted in food facilities

    Science.gov (United States)

    The phase out of methyl bromide for the treatment of structures where grain is milled and processed has triggered a need to evaluate the effectiveness of alternative structural treatments such as sulfuryl fluoride and heat. The red flour beetle (Tribolium castaneum (Herbst)) (RFB) and confused flour...

  11. Residual efficacy of methoprene for control of Tribolium castaneum (Coleoptera: Tenebrionidae) larvae at different temperatures on varnished wood, concrete, and wheat

    Science.gov (United States)

    The residual efficacy of the juvenile hormone analogue, methoprene (Diacon II), was evaluated in bioassays using larvae of Tribolium castaneum (Herbst) exposed on varnished wood or unsealed concrete treated with a liquid formulation and held at different temperatures. When these surfaces were stored...

  12. Efficacy of Spinosad and Abamectin against Different Populations of Red Flour Beetle (Tribolium castaneum Herbst in Treated Wheat Grain

    Directory of Open Access Journals (Sweden)

    Goran Andrić

    2013-01-01

    Full Text Available The efficacy of spinosad and abamectin against T. castaneum adults from a laboratorypopulation with normal susceptibility to contact insecticides and against malathion-resistantpopulations from Nikinci and Jakovo was tested in the laboratory (25±1ºC and 60±5%r.h.. The insecticides were applied to 500 g of untreated wheat grain for each of the followingapplication doses: 0.25, 0.5, 1.0, 2.5 and 5.0 mg a.i./kg. After treatment, wheat was dividedinto three equal subsamples and 50 T. castaneum adults from each of the three test populationswere released the next day into jars for each dose. Mortality was evaluated after 7,14 and 21 days of exposure to treated wheat grain.Generally, higher concentrations and longer exposure periods resulted in higher efficacyof both insecticides, but abamectin was significantly more effective than spinosadagainst all three tested populations. After 7 days of exposure, mortality did not exceed30% in any test variant. Fourteen days after treatment with the highest dose (5 mg/kgof spinosad, mortality was highest (75% in the laboratory population, while treatmentwith the same dose of abamectin achieved the highest mortality (58% in the laboratoryand Jakovo populations. After 21 days, spinosad applied at the rate of 5 mg/kg was mosteffective (97% mortality in the laboratory population, while 88% efficacy was recorded inJakovo population and 87% in Nikinci population. Abamectin doses of 2.5 and 5 mg/kgcaused high adult mortality of 94-100% in the laboratory and Jakovo populations, and a significantlylower mortality in Nikinci population (75 and 86%, respectively.Statistically significant differences in the efficacy of spinosad, and particularly ofabamectin, were detected among the three tested populations, the greatest differencebeing between the laboratory and Nikinci populations, which clearly indicates that resistanceof T. castaneum adults to malathion had a significant influence.

  13. Evaluation of synergized pyrethrin aerosol for control of Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Kharel, Kabita; Arthur, Frank H; Zhu, Kun Yan; Campbell, James F; Subramanyam, Bhadriraju

    2014-02-01

    Aerosol insecticides are being used in flour mill pest management programs, but there is limited information on their efficacy on different insect life stages. In this study, we evaluated the efficacy of synergized pyrethrin applied as an aerosol against eggs, larvae, pupae, and adults of the red flour beetle, Tribolium castaneum (Herbst), and the confused flour beetle, Tribolium confusum Jacquelin du Val. Effects of direct and indirect exposure were evaluated by exposing each life stage to the aerosol and then transferring to untreated flour, transferring untreated insects to treated flour, or exposing both the insects and the flour to the aerosol. The aerosol produced >88% mortality of both species and all life stages when insects were directly treated and transferred to either treated or untreated flour. Mortality was significantly reduced when insects were either treated together with flour or untreated insects were transferred to treated flour (indirect exposure to the aerosol). Larvae and adults of both species were more tolerant compared with eggs and pupae. Recovery of moribund adults in the indirect exposure treatments was greater compared with recovery of moribund insects in the direct exposure treatments. Good sanitation before aerosol application could facilitate direct exposure of insects and thus increase aerosol efficacy inside flour mills.

  14. Chemical composition of four essential oils from Eupatorium spp: Biological activities toward Tribolium castaneum (Coleoptera: Tenebrionidae Composición química de cuatro aceites esenciales provenientes de Eupatorium spp. y su toxicidad para Tribolium castaneum (Coleoptera: Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Hugo G. Lancelle

    2009-12-01

    Full Text Available Toxic and repellent properties of whole essential oils from four Eupatorium (Asteraceae species (E. buniifolium Hook. et Arn, E. inulaefolium Kunth, E. arnottii Baker, and E. viscidum Hook. & Arn were investigated in different concentrations toward Tribolium castaneum Herbst adults. The essential oils were isolated by hydrodistillation techniques from the aerial parts. The analysis was performed by GC-FID and GC-MS methods. Contact toxicity assays showed that all the evaluated essential oils were toxic. Furthermore, in all the cases mortality was dose dependent. The main repellency was observed for the essential oil recovered from E. buniifolium.Se evaluaron las propiedades tóxicas y repelentes de los aceites esenciales de cuatro especies del género Eupatorium (Asteraceae: E. buniifolium Hook. et Arn, E. inulaefolium Kunth, E. arnottii Baker y E. viscidum Hook. & Arn, en diferentes concentraciones frente a adultos de Tribolium castaneum Herbst. Los aceites esenciales se aislaron de las partes aéreas de las plantas, mediante técnicas de hidrodestilación y se analizaron por los métodos GC-FID y GC-MS. Los ensayos de toxicidad por contacto demostraron que todos los aceites fueron tóxicos y la mortalidad fue, en todos los casos, dependiente de la dosis. El aceite esencial de E. buniifolium presentó la mayor actividad repelente.

  15. The Presence of Flour Affects the Efficacy of Aerosolized Insecticides used to Treat the Red Flour Beetle, Tribolium castaneum

    OpenAIRE

    Toews, Michael D; Campbell, James F.; Arthur, Franklin H.

    2010-01-01

    Experiments were conducted in tightly sealed pilot scale warehouses to assess the efficacy of common aerosolized insecticides on all life stages of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) when exposed in dishes containing 0 to 2 g of wheat flour either under pallets or out in the open. Petri dishes containing 0, 0.1, 1, or 2 g of flour were prepared with 25 eggs, 3rd instars, pupae, or adults and then immediately treated with aerosolized solvent, Pyrethrins, or esfenvalerate....

  16. Ojo blanco (obl : un gene marcador del grupo de ligamiento IV en Tribolium castaneum Herbst Ojo blanco (obl: un gene marcador del grupo de ligamiento IV en Tribolium castaneum Herbst

    Directory of Open Access Journals (Sweden)

    Nuñez Fernando

    1988-06-01

    Full Text Available

    Some genetic aspects of a spontaneous mutation in T. castaneum, discovered in the Department of Biology of National University of Colombia (Bogota, were studied. The mutant gene christened "ojo blanco" (obl, is recessive autosomic, of excellent viability, complete penetrance and variable expression. The gene is phenotipically characterized by a depigmentation of the compound eyes and can be observed from the larval stage. Linkage tests with marker mutants for different groups were carried out. The marker "sooty", of the linkage group IV, gave positive results. The distance was estimated from 2843 descendants obtained from backcrosses in repulsion plase and 1630 in coupling phase. This distance was worked out as 15.64 ± 0.92 units between obl ands. It was not possible to determine if obl is on the left or the right hand side of s. Nor discrepancies between sexes, neither between phases were found in the recombination frequencies for the region between the two loci of chromosome IV.

    Se estudiaron algunos aspectos genéticos de una mutación espontanea en T. casteneum, descubierta en el Departamento de Biología de la Universidad Nacional de Colombia (Bogotá. EI gene mutante llamado "ojo blanco" (obl es autosómico recesivo, de excelente viabilidad, penetrancia completa y expresividad variable. Fenotípicamente se caracteriza por una despigmentación de los ojos compuestos; puede observarse desde el estado de larva. Se le hicieron pruebas de ligamiento con marcadores para diferentes grupos. Se obtuvieron resultados positivos con el marcador "sooty" del grupo de ligamiento IV. La  distancia se calcu1ó a partir de 2.843 descendientes de retrocruzas en posición de repulsión y 1.630 descendientes de retrocruzas en posición de acoplamiento. Esta distancia fue de 15.ó4 ± 0.92 unidades entre obl ys. No se pudo determinar si obl esta a la izquierda 0 a la

  17. Toxic and Repellent effecto of Harmal (Peganum harmala L. Acetonic Extract on Several Aphids and Tribolium castaneum (Herbst Efecto Tóxico y Repelente del Extracto Acetónico de Harmal (Peganum harmala L. sobre varias especies de Áfidos y Tribolium castaneum (Herbst

    Directory of Open Access Journals (Sweden)

    Elham Salari

    2012-03-01

    Full Text Available To reduce the dependence on the sometimes unwise use of synthetic pesticides in fruit and vegetable plantations, the toxicity and repellence of Peganum harmala L. (Zygophyllaceae acetonic seed extract was assayed against several insect pests. For contact toxicity, 3- to 4-d-old individuals of Aphis fabae Scopoli, A. gossypii Glover, A. nerii Boyer de Fonscolombe, and Myzus persicae (Sulzer were included, as well as 1- to 7-d-old adult Tribolium castaneum (Herbst. Repellent effect experiments were conducted on adult, 1- to 2- and 3- to 4-d old M.persicae individuals. At 60 mg mL4, the topical bioassay mortality percentage was significantly higher in A. gossypii than in A. fabae and A. nerii after 12-72 h. Mortality of the treatments on M.persicae was 87.1% and 90.0% after 24 and 48 h, respectively, and significantly higher than A. fabae and A. nerii during this period. At 60 mg mL-1, the mortality of T. castaneum was much lower than that of the aphid species. The highest repellent index (over 72% was observed on 1- to 2-d-old M. persicae individuals.Para reducir la dependencia de los pesticidas sintéticos en plantaciones frutales y hortalizas, se realizó un ensayo para medir la toxicidad y repelencia de un extracto acetónico obtenido a partir de semillas de Peganum harmala L. (Zygophyllaceae contra diferentes especies de plagas. Para evaluar la toxicidad del extracto al contacto con los insectos, se incluyeron individuos de 3-4 d de edad de Aphis fabae Scopoli, Aphis gossypii Glover, Aphis nerii Boyer de Fonscolombe, y Myzus persicae (Sulzer, así como adultos 1-7 d de edad de Tribolium castaneum (Herbst. Experimentos para medir el efecto repelente se llevaron a cabo con individuos de 1-2 y 3-4 d de edad de M. persicae. En los resultados de los bioensayos tópicos el porcentaje de mortalidad fue significativamente mayor en la especie A. gossypii que en A. fabae y A. nerii, después de 12-72 h con una concentración de 60 mg mL-1. La mortalidad

  18. Investigation of Natural Plant Aegle marmelos Essential Oil Bioactivity on Development and Toxicity of Tribolium castaneum (Coleoptera: Tenebrionidae

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    Bhuwan Bhaskar Mishra

    2016-01-01

    Full Text Available Background: The uncontrolled use of synthetic chemicals is a great hazard for the environment and consumers. Methodology: In the present study, essential oil from leaves of Aegle marmelos was isolated by hydro-distillation and tested their insecticidal activity against Tribolium castaneum (Herbst. Results: The results showed that the essential oil of A. marmelos have fumigant toxicity, oviposition and developmental inhibitory activity against T. castaneum. The percentage mortality increased with increasing exposure time and concentration. The median lethal concentration (LC50 of A. marmelos essential oil at 48 h was 14.172 and 17.752 FL against larvae and adults of T. castaneum, respectively. The essential oil significantly reduced oviposition (F3,20 = 304.734 in adults and also reduced pupation (F3,20 = 137.442 and adult emergence (F3,20 = 225.619 in larvae when fumigated with sub-lethal concentration. The percent grains infection was reduced 83.66% at 60% of sub-lethal concentration of 24 h LC50. Fumigation of insect with sub-lethal concentration of A. marmelos essential oil inhibited AChE activity. Reduction in AChE activity was 81.48 and 54.32% of the control, after 24 h of fumigation with sub-lethal concentration. Conclusion: In conclusion, this essential oil probably induces toxicity in insect by inhibiting AChE activity.

  19. Composition, Repellent, and Insecticidal Activities of Two South American Plants against the Stored Grain Pests Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae)

    OpenAIRE

    Benzi, Verónica; Stefanazzi, Natalia; Murray, Ana Paula; Werdin González, Jorge O.; Ferrero, Adriana

    2014-01-01

    As part of a screening program to evaluate the biological activity of indigenous plants, we report the composition and the bioactivity of essential oils (EOs) extracted from Té de Burro Aloysia polystachya [(Griseb.) Moldenke] and Lemon Verbena Aloysia citriodora [Palau] against two of the most widespread secondary pests of stored products, the red flour beetle Tribolium castaneum [Herbst] and the confused flour beetle Tribolium confusum [Jacqueline du Val]. Analysis by gas chromatography-mas...

  20. The influence of different temperature on the crawl and flight initiation of Tribolium castaneum (Herbst)%温度对赤拟谷盗爬行和起飞活动的影响

    Institute of Scientific and Technical Information of China (English)

    李兆东; 王殿轩; 乔占民

    2011-01-01

    在不同温度下研究了赤拟谷盗Tribolium castaneum(Herbst)在固体物面、设定粮面、温度均匀的粮(小麦)柱和具有温度梯度粮柱内爬行扩散速度、起飞温度等.主要结果为:赤拟谷盗在17℃开始有爬行为,25℃开始有飞行行为,在15~25℃时水平爬行速度和15 ~ 20℃时竖直运动速度与温度呈显著正相关,在无障碍固体物面上的爬行速度为在粮面上速度的3.9~5.9倍,在水平粮柱内的扩散速度约为在竖直粮柱内扩散速度的1.2~3.1倍,赤拟谷盗的适宜起飞温度在25~30℃之间.%The influence of temperature on the dispersal rate and flight initiation of Tribolium castaneum (Herbst) adults was studied at different temperatures and an different substrates. The dispersal rate was monitored both on a solid surface and on wheat in a perfipex groove at both uniform temperatures and over a temperature gradient. The results indicate that the minimum temperature that initiated crawling was 17℃. Both on the solid surface and wheat bulk. The minimum temperature for flight initiation was 25 ℃ on both substrates. The dispersal rate of adults significantly increased with temperature within the range of 15 - 25℃ for horizontal movement, and within the range of 15 - 20% for vertical movement in the wheat column. The dispersal rate on the solid surface was 3. 9 -5. 9 times higher than that on wheat. The dispersal rate in wheat columns was 1.2 - 3. 1 limes higher than in vertical columns without wheat. The ideal flight temperature was 25 -30 ℃ for both male and female T. Castaneum.

  1. Elucidating the Common Generalist Predators of Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae) in an Organic Apple Orchard Using Molecular Gut-Content Analysis.

    Science.gov (United States)

    Schmidt, Jason M; Szendrei, Zsofia; Grieshop, Matthew

    2016-01-01

    Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), plum curculio, is a serious direct pest of North American tree fruit including, apples, cherries, peaches and plums. Historically, organophosphate insecticides were used for control, but this tool is no longer registered for use in tree fruit. In addition, few organically approved insecticides are available for organic pest control and none have proven efficacy as this time. Therefore, promoting biological control in these systems is the next step, however, little is known about the biological control pathways in this system and how these are influenced by current mechanical and cultural practices required in organic systems. We used molecular gut-content analysis for testing field caught predators for feeding on plum curculio. During the study we monitored populations of plum curculio and the predator community in a production organic apple orchard. Predator populations varied over the season and contained a diverse assemblage of spiders and beetles. A total of 8% of all predators (eight Araneae, two Hemiptera, and six Coleoptera species) assayed for plum curculio predation were observed positive for the presence of plum curculio DNA in their guts, indicating that these species fed on plum curculio prior to collection Results indicate a number of biological control agents exist for this pest and this requires further study in relation to cultural practices. PMID:27348005

  2. Effects of Flour and Milling Debris on Efficacy of Beta-Cyfluthrin for Control of Tribolium castaneum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Arthur, Frank H; Starkus, Laura A; Mckay, Tanja

    2015-04-01

    Laboratory and field studies were conducted to determine if accumulation of a flour food source or milling debris affected residual efficacy of beta-cyfluthrin for control of Tribolium castaneum (Herbst), the red flour beetle. In the laboratory study, the high label rate of 20 mg active ingredient (AI)/m2 gave effective control for 8 wks, regardless of whether or not the concrete was sealed prior to application or the presence of flour contamination. However, with the low label rate of 10 mg AI/m2, the flour apparently absorbed the insecticide residues from the treated surface, and sealing the concrete did not have a beneficial effect on efficacy. Two field studies with the low label rate were conducted during autumn of 2012 and 2013 and summer of 2013, using only unsealed concrete. Accumulated milling debris caused a reduction in efficacy in the autumn studies, as shown by increased time to 100% knockdown, decreased mortality, and decreased residual efficacy. There was no such corresponding decrease in residual efficacy in the summer study. Overall, results of both studies show that accumulated food and milling debris can absorb residue of beta-cyfluthrin from a treated surface and have a negative impact on residual efficacy, particularly with the low label rate of 10 mg AI/m2.

  3. IRRADIATION EFFECT OF ELECTRONIC BEAM ON RED FLOUR BEETLE,TRIBOLIUM CASTANEUM (HERBST)%电子束对赤拟谷盗辐照效应的试验研究

    Institute of Scientific and Technical Information of China (English)

    李淑荣; 王殿轩; 温贤芳; 原锴

    2004-01-01

    采用了ИЛУ-6M2型电子加速器产生的电子束对赤拟谷盗Tnbolium castaneum(Herbst)成虫、卵、幼虫和蛹进行了辐照效应试验.结果表明,赤拟谷盗不同发育阶段对电子束辐照的敏感度不同,卵最为敏感,高于210 Gv的辐照的卵不能孵化.305 Gy以上的剂量可以完全阻止赤拟谷盗的幼虫发育为成虫;高于305 Gy辐照的蛹虽然能羽化为成虫,但羽化后的成虫不能正常存活;518 Gy以上的剂量辐照的成虫在4周后100%死亡.518 Gy辐照剂量可以作为辐照防治赤拟谷盗的有效剂量.

  4. The sperm ultrastructure and spermiogenesis of Tribolium castaneum (Coleoptera: Tenebrionidae) with evidence of cyst degeneration.

    Science.gov (United States)

    Dias, Glenda; Lino-Neto, José; Mercati, David; Dallai, Romano

    2015-06-01

    Previous studies on the spermatogenesis of tenebrionid beetles showed the unusual formation of two antiparallel sperm bundles per cyst. In this work we reported this feature also in Tribolium castaneum using light and transmission electron microscopy. The sperm structure of T. castaneum, similar to other tenebrionids, consists of a three-layered acrosome, an elongated nucleus and a flagellum with a 9+9+2 axoneme, two accessory bodies and two asymmetric mitochondrial derivatives. The presence of two antiparallel sperm bundles per cyst also in Meloidae and Rhipiphoridae suggests that it is a strong trait synapomorphic for Tenebrionoidea. The huge degeneration of whole sperm cells in several cysts of testes during spermiogenesis is also described.

  5. Concentration dependent toxicokinetics of copper in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Bednarska, Agnieszka J; Stępień, Katarzyna

    2015-11-01

    To predict internal metal concentrations in animals under specific environmental exposures, the relationship between the exposure concentrations and values of toxicokinetic parameters must be known. At high exposure levels, the availability of carriers transporting metal ions through cellular membranes may become limited, thereby decreasing the assimilation rates (k A ). Furthermore, increased metal concentrations in food may result in greater damage to the gut and reduce the assimilation efficiency and/or increase the elimination rate (k E ). Therefore, k A should decrease and k E should increase with increasing metal concentrations. In fact, our study on Tribolium castaneum exposed to Cu at 500, 1000, 2000 and 4000 mg kg(-1) of dry flour showed that with increasing Cu concentrations, k A decreased from 0.0042 day(-1) at 500 mg kg(-1) to 0.0026 day(-1) at 4000 mg kg(-1) in females and from 0.0029 to 0.001 day(-1) in males and k E increased from 0.027 to 0.064 day(-1) and from 0.018 to 0.04 day(-1) in females and males, respectively. Significant differences in k A between the sexes were observed at 2000 and 4000 mg kg(-1), whereas significant differences between treatments were found for k A in males. Copper was efficiently regulated by T. castaneum: an eightfold increase in exposure concentrations resulted in only a ca. twofold increase in the internal concentration. No Cu effect on the respiratory metabolism of T. castaneum was found.

  6. Concentration dependent toxicokinetics of copper in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Bednarska, Agnieszka J; Stępień, Katarzyna

    2015-11-01

    To predict internal metal concentrations in animals under specific environmental exposures, the relationship between the exposure concentrations and values of toxicokinetic parameters must be known. At high exposure levels, the availability of carriers transporting metal ions through cellular membranes may become limited, thereby decreasing the assimilation rates (k A ). Furthermore, increased metal concentrations in food may result in greater damage to the gut and reduce the assimilation efficiency and/or increase the elimination rate (k E ). Therefore, k A should decrease and k E should increase with increasing metal concentrations. In fact, our study on Tribolium castaneum exposed to Cu at 500, 1000, 2000 and 4000 mg kg(-1) of dry flour showed that with increasing Cu concentrations, k A decreased from 0.0042 day(-1) at 500 mg kg(-1) to 0.0026 day(-1) at 4000 mg kg(-1) in females and from 0.0029 to 0.001 day(-1) in males and k E increased from 0.027 to 0.064 day(-1) and from 0.018 to 0.04 day(-1) in females and males, respectively. Significant differences in k A between the sexes were observed at 2000 and 4000 mg kg(-1), whereas significant differences between treatments were found for k A in males. Copper was efficiently regulated by T. castaneum: an eightfold increase in exposure concentrations resulted in only a ca. twofold increase in the internal concentration. No Cu effect on the respiratory metabolism of T. castaneum was found. PMID:26169625

  7. Lethal dose determination of Cobalt-60 for adult Tribolium castaneum (Coleoptera: Tenebrionidae) and Cryptolestes ferrugineus (Coleoptera: Laemophloeidae)

    Energy Technology Data Exchange (ETDEWEB)

    Farias, Anderson Aparecido, E-mail: potenza@biologico.sp.gov.br [Instituto Biologico, Centro de Pesquisa e Desenvolvimento de Protecao Ambiental, Sao Paulo, SP (Brazil); Potenza, Marcos Roberto, E-mail: fcreis@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Reis, Fabricio Caldeira; Sato, Mario Eidi, E-mail: mesato@biologico.sp.gov.br [Instituto Biologico, Centro Experimental Central, Laboratorio de Acarologia, Campinas, SP (Brazil)

    2015-07-01

    The insect infestation is a major problem in the grain storage. The pesticides are most widely used method for disinfestation and prevention. Treatment with gamma irradiation may increase the product shelf life without encountering formation of waste can be used in packaged foods and ready for commercialization, representing an important alternative to the use of pesticides. This study aimed to determine the immediate lethal dose of gamma radiation for adults of Tribolium castaneum and Cryptolestes ferrugineus. The study was conducted in the Instituto Biologico and the radiations held at the Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN, Sao Paulo city, using a multipurpose irradiator Cobalt-60 with 3.31 dose rate and 3.23 kGy in months of November/2014 and January/2015. Each experimental unit consisted of 20 adult insects, confined in a 10 mL polyethylene container. The experimental plots in number 10 per dose were subjected to increasing doses of gamma radiation: 0; 0.5; 0.75; 1.0; 1.25; 1.50; 1.75; 2.00; 2:50 and 3.00 kGy. Mortality was assessed within two to four hours after irradiation. The data were submitted to Probit analysis, using the POLO PLUS program. The LD90 to control 90% (LD90) to control adult Cryptolestes ferrugineus and Tribolium castaneum were 2.73 and 2.91 kGy, respectively. (author)

  8. A cell line derived from the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Goodman, Cynthia L; Stanley, David; Ringbauer, Joseph A; Beeman, Richard W; Silver, Kristopher; Park, Yoonseong

    2012-08-01

    The red flour beetle, Tribolium castaneum, is a model organism for agricultural and medical research and its complete genome is sequenced. We established a continuously replicating T. castaneum cell line to complement existing physiological, genetic, and genomic research tools. We set up trial cell cultures from egg, pupa, and adult stages as tissue sources and incubated them in six separate cell culture media to determine the optimal combination of tissue source and medium for cell replication. Our most promising culture was generated by co-culturing adult (∼75 %) and pupal tissues in EX-CELL 420 medium containing 9 % FBS. Our new cell culture is designated BCIRL-TcA-CLG1 (TcA) and it has been subcultured more than 90 times. Amplification of genomic DNA with species-specific primers yielded DNA fragments of the expected sizes and with sequences identical to those from the published Tribolium genome. Additionally, we characterized this line using DNA fingerprinting (DAF-PCR) and compared it with three other coleopteran cell lines and its conspecific pupae to confirm identity. Its doubling time is 155.2 hr. Early passages consisted of attached cells and vesicles in suspension, whereas later passages consisted primarily of attached, spherical cells. Similar to other established cell lines, the ploidy of TcA cells was variable, ranging from 20 chromosomes/cell (diploid) to above 30 chromosomes/cell. TcA cells withstood incubation at 40°C for 1 h with no decrease in viability. We recorded increased levels of one heat shock protein (43 kDa) and of the hsp68a transcript following exposure to 40°C. Taken together, this represents the first report of a continuously replicating T. castaneum cell line. We expect the BCIRL-TcA-CLG1 line will become a useful tool in Tribolium research.

  9. FIELD TRIAL OF PHOSPHINE FUMIGATION ON HIGH RESISTANT STRAINS OF TRIBOLIUM CASTANEUM(HERBST)IN STORED WHEAT IN HORIZONTAL STORAGE%实仓熏蒸磷化氢高抗性赤拟谷盗的研究

    Institute of Scientific and Technical Information of China (English)

    朱建民; 王殿轩; 孙颖; 宋涛; 刘建岭; 杨堃; 张龙; 唐多; 李兆东

    2011-01-01

    在高大平房仓储藏小麦中采用熏蒸试验比较了磷化氢对抗性倍数分别为1、2.5、64和206倍的赤拟谷盗品系杀虫效果.主要结果为:在施药后第2天磷化氢浓度达到210 mL/m3以上,且在经过210~520 mL/m3上升和520~350 mL/m3的变化情况下,抗性2.5倍的品系在3d内全部死亡,抗性64倍的害虫完全死亡时间为6 d,抗性206倍的成虫则在第9天完全死亡.无抗性的害虫经培养也没有出现子代成虫,抗性品系即时死亡率达96%的样品经后期培养其中出现少量子代成虫,但其繁殖力明显小于未进行熏蒸的同品系害虫.结果表明,密闭性较好的高大平房仓中很小的剂量即可保持较高的磷化氢浓度,也可在较短时间内杀死高抗性赤拟谷盗.%A field fumigation of phosphine was carried out on four strains of Tribolium castaneum (Herbst) with resistant factor (Rf) of 1、2.5、64 and 206 in stored wheat in horizontal storage.The results showed that the full mortality time of the strain with Rf 2.5 was three days, the full mortality time of the strain with Rf 64 was six days, and the full mortality time of the strain with Rf 206 was nine days under the condition that the phosphine concentration reached 210 mL/m3 on the second day after fumigation, then rose to 210 mL/m3 and 520 mL/m3 and changed between 520 mL/m3 and 350 mL/m3.There were no progeny reproduced by the adults of low level resistance strains after fumigation forty days.The resistant strains with 96% sudden mortality rate reproduced a small amount of adults of the offspring after cultivation, but the reproductive ability was remarkably lower than that of the non-fumigated insects of the same strains.The results indicated that higher phosphine concentration could be kept in a high and large flat warehouse by adding a very low dosage of phosphine, and Tribolium castaneum (Herbst) with high resistance could be killed in a short time.

  10. Toxicological and Biochemical Characterizations of Acetylcholinesterase in Tribolium castaneum (Herbst)%赤拟谷盗乙酰胆碱酯酶生化及毒理学特性研究

    Institute of Scientific and Technical Information of China (English)

    刘国瑛; 柴玉鑫; 魏丹丹; 王进军

    2006-01-01

    为了阐明赤拟谷盗[Tribolium castaneum(Herbst)]体内乙酰胆碱酯酶(AChE)在其各虫态间的变化关系,以期为该虫的综合治理提供一定的理论依据,本试验采用微量滴度酶标板法,分别对其10d龄和20d龄幼虫、蛹及成虫体内AChE的生化及毒理学特性进行了较为系统的研究.结果表明,赤拟谷盗各虫态AChE酶源蛋白含量、酶活力和比活力差异显著,酶源蛋白含量从低到高依次为:10d龄幼虫<20d龄幼虫<成虫<蛹;AChE活力和比活力依次为:成虫<蛹<10d龄幼虫<20d龄幼虫.酶动力学分析发现,AChE米氏常数Km值大小依次为:蛹<成虫<10d龄幼虫<20d龄幼虫,但成虫和蛹之间的差异不显著.此外,幼虫AChE的最大反应速度Vmax亦显著高于成虫和蛹.通过对幼虫AChE离体抑制作用测定表明,毒扁豆碱、马拉氧磷和西维因对20d龄幼虫的抑制中浓度I50均高于10d龄幼虫,说明20d龄幼虫体内的AChE对3种抑制剂的抑制作用更不敏感.

  11. COMPARATIVE STUDY ON ENERGY SOURCES IN THE PH3 RESISTANT AND SUSCEPTIBLE STRAINS OF TRIBOLIUM CASTANEUM(HERBST)%赤拟谷盗磷化氢抗性与体内能源物质含量的关系研究

    Institute of Scientific and Technical Information of China (English)

    陈晨; 任艺; 王进军

    2008-01-01

    多糖、甘油三酯和可溶性蛋白是昆虫体内主要的三种能源物质.本研究通过乙酰丙酮法、蒽酮比色法和考马斯亮蓝G-250法测定了赤拟谷盗 Tribolium castaneum(Herbst)磷化氢(PH3)抗性品系与敏感品系成虫、蛹和幼虫体内三种能源物质的含量.结果表明,除幼虫体内的甘油三酯和多糖外,其它的测定结果均为敏感品系最高,说明抗性品系赤拟谷盗的能源物质储量可能产生了一定程度的缺失.

  12. Chaves para a identificação dos principais Coleoptera (Insecta associados com produtos armazenados Keys for the identification of Coleoptera (Insecta associated with stored products

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Valle da Silva Pereira

    2001-03-01

    Full Text Available An illustrated key to identify nine families of Coleoptera commonly found in stored products is presented. Keys for the identification of Anobiidae [Lasioderma serricorne (Fabricius, 1792, Stegobium paniceum (Linnaeus, 1761], Bruchidae [Acanthoscelides obtectus (Say, 1831, Zabrotes subfasciatus (Boheman, 1833], Curculionidae [Sitophilus oryzae (Linnaeus, 1763, S. zeamais Motschulsky, 1885], Silvanidae [Ahasverus advena (Waltl, 1832, Cathartus quadricollis (Guérin, 1892, Oryzaephilus mercator (Fauvel, 1889, O. surinamensis (Linnaeus, 1758] and Tenebrionidae [Gnathocerus cornutus (Fabricius, 1798, Tribolium castaneum (Herbst, 1797, T. confusum du Val, 1868] are also provided. These keys cover the most frequent Coleoptera found in stored products, specially grains, and are to the adult stage only. Illustrations of external morphology and general characteristics are provided for each species reported.

  13. Influence of gamma radiation, continuous light and complete darkness on the reproductive potential of red flour beetle, Tribolium Castaneum (Herbst), (Coleoptera; Tenebrionidae)

    Energy Technology Data Exchange (ETDEWEB)

    Khattak, S.U.K. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan)); Jilani, G. (Nuclear Inst. for Agriculture and Biology, Faisalabad (Pakistan))

    The influence of gamma radiation, continuous light and complete darkness singly as well as in combination on the reproductive potential of Tribolium castameum was studied under controlled laboratory conditions. It was found that among the single treatments radiation gave the best results followed by continuous light and continuous darkness, while in the combined treatments the effect of radiation in either combination of light or dark was complementary and both the fecundity and fertility were adversely affected, however, the effect on fertility was more pronounced than the fecundity. Radiation with continuous light proved to be the best of all combinations. Females were more sensitive than males in all types of treatments.

  14. COMPARISON OF SPECIFIC ACTIVITY OF CATALASE IN THE PHOSPHINE-RESISTANT AND PHOSPHINE-SUSCEPTIBLE STRAINS OF RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)%赤拟谷盗磷化氢抗性和敏感品系的过氧化氢酶活性比较

    Institute of Scientific and Technical Information of China (English)

    王殿轩; 王利丹; 高希武

    2009-01-01

    比较研究了3个对磷化氢抗性有明显差异的赤拟谷盗Tribolium castaneum(Herbst)品系与敏感品系虫体内过氧氢酶的比活力,主要结果为当赤拟谷盗各品系对磷化氢的抗性系数分别为1倍、2.42倍、270.81倍和352.80倍时,虫体内过氧化氢酶的比活力比值相应地为1、1.27、3.88、5.25.结果表明赤拟谷盗虫体内过氧氢酶的活性大小与其对磷化氢的抗性水平高低呈正相关,害虫对磷化氢的抗性可能与过氧化氢酶的活性增加有关.

  15. Toxicidade da combinação de dióxido de carbono e fosfina sob diferentes temperaturas para Tribolium castaneum Toxicity of the carbon dioxide and phosphine combination to Tribolium castaneum under different temperatures

    Directory of Open Access Journals (Sweden)

    Raimundo W. S. Aguiar

    2010-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da temperatura sobre a toxicidade da combinação de dióxido de carbono e fosfina, para os estágios de desenvolvimento de Tribolium castaneum (Herbst (Coleoptera: Tenebrionidae. A toxicidade da combinação de 5% de dióxido de carbono e 1 g m-3 de fosfina para os estágios de ovo, larvas de 5, 10 e 15 dias, pupa e adulto de T. castaneum, foi estudada nas temperaturas de 25, 30, 35, 40 e 45 °C, por meio de estimativas dos tempos de exposição letais para 50 e 95% dos insetos (TL50 e TL95. Curvas tempo-resposta foram estabelecidas mediante bioensaios com períodos crescentes de exposição à combinação do dióxido de carbono com a fosfina. Observou-se que os TL50 e TL95 reduziram com a elevação da temperatura em todos os estágios de T. castaneum avaliados. O estágio de larva de cinco dias foi a mais susceptível à combinação de dióxido de carbono e fosfina. De acordo com os resultados, a combinação do dióxido de carbono com a fosfina é alternativa potencial para diminuir a quantidade de fosfina aplicada em produtos armazenados, por apresentar alta toxicidade para todos os estágios de T. castaneum sob diferentes temperaturas.The objective of this work was to assess the effect of temperature on the toxicity of the carbon dioxide-phosphine combination for the developmental stages of Tribolium castaneum (Herbst (Coleoptera: Tenebrionidae. The toxicity of combination of 5% carbon dioxide and 1 g m-3 phosphine in the developmental stages of egg, larvae of 5, 10 and 15 days, pupae and adult of T. castaneum was studied under the temperatures of 25, 30, 35, 40 and 45 °C, through the estimation of lethal insect exposure times of 50 and 95% (LT50 and LT95. For that, time-response curves were established through bioassays with increasing periods of exposure to the combination of carbon dioxide and phosphine. A reduction of LT50 and LT95 was observed with temperature increase in all

  16. 球孢白僵菌九个菌株对赤拟谷盗成虫的毒效%Efficacy of nine isolates of Beauveria bassiana against adults of Tribolium castaneum (Coleoptera: Tenebrionidae)

    Institute of Scientific and Technical Information of China (English)

    Hediyeh GOLSHAN; Moosa SABER; Farzad MAJIDI-SHILSAR; Farrokh KARIMI

    2013-01-01

    Beauveria bassiana (Balsamo) Vuillemin is one of the most important entomopathogenic fungi and has been widely used for many insect pests all over the world.In this study,the pathogenicity of nine isolates of B.bassiana was evaluated against adults of Tribolium castaneum (Herbst).Fifteen adults of the pest were submerged into the four suspensions (1 × 106,1 × 107,1 × 10s and 1 × l09 conidia/mL) of different isolates of B.bassiana for 20 s,and the mortality was recorded daily for 14 d.Results showed that IRAN 440C and IRAN 187C isolates had the lowest (5.04 × 107 conidia/mL) and highest (5.05 ×108 conidia/mL) LCs0,and DEBI 005 and DEBI 014 isolates had the shortest (2.88 d) and longest (4.96 d) LT50,respectively.According to the LC5o and LT50 values and the mortality,IRAN 440C is a perfect isolate for using in controlling the pest.%球孢白僵菌Beauveria bassiana (Balsamo) Vuillemin是最重要的昆虫病原真菌,广泛用于防治世界各地的多种害虫.本研究评价了球孢白僵菌9个菌株对赤拟谷盗Tribolium castaneum (Herbst)成虫的致病性.将15头赤拟谷盗成虫浸入到4个浓度(1×106,l×107,l×lo8和l×109个分生孢子/mL)的白僵菌菌株中20 s,14 d内每日记录成虫的死亡率.结果表明:IRAN 440C菌株对赤拟谷盗成虫的LC50最低(5.04 ×l07个分生孢子/mL),IRAN187C菌株的最高(5.05 ×lo8个分生孢子/mL);DEBI 005菌株对赤拟谷盗成虫的LT5o最短(2.88 d),DEBI 014菌株的最长(4.96 d).根据LC50,LT50和死亡率结果得出IRAN 440C是防治这一害虫的理想菌株.

  17. 营养质量对雄性赤拟谷盗生长发育、抗饥饿和资源再获取能力的影响%Effect of nutrition quality on the development, starvation resistance and resource reacquisition ability of male Tribolium castaneum (Herbst)

    Institute of Scientific and Technical Information of China (English)

    明庆磊; 冯照军; 程超

    2013-01-01

    昆虫的生长发育、抗饥饿和资源再获取能力对其生存、生殖和性选择非常重要.本文研究了营养质量对赤拟谷盗Tribolium castaneum (Herbst)生长发育、抗饥饿和资源再获取能力的影响.结果表明,三个营养处理下雄性赤拟谷盗的生长发育性状(幼虫重、发育历期、蛹重和成虫重)均存在显著性差异,在高营养质量下生长的雄性赤拟谷盗发育更快,体重更大,且各生长发育性状间存在显著性相关.营养质量对雄性赤拟谷盗成虫的抗饥饿能力没有显著影响,但对其资源再获取能力有显著影响,表现为在高营养质量下生长的雄性赤拟谷盗成虫比在低营养质量下生长的具有更高的资源再获取能力.雄性赤拟谷盗抗饥饿能力和资源再获取能力之间存在显著性相关,但是雄性赤拟谷盗抗饥饿能力和资源再获取能力除了与发育历期存在显著性相关外,与其它生长发育性状不存在显著性相关.本文还对这些实验结果的性选择意义进行了讨论.

  18. Impact of varying levels of sanitation on mortality of Tribolium castaneum eggs and adults during heat treatment of a pilot flour mill.

    Science.gov (United States)

    Brijwani, Monika; Subramanyam, Bhadriraju; Flinn, Paul W

    2012-04-01

    The influence of sanitation on responses of life stages of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), an economically important pest in flour mills, was investigated in a pilot flour mill subjected to two, 24-h heat treatments. One hundred eggs or 100 adults of T. castaneum were exposed inside each 20-cm diameter by 15-cm high PVC rings holding 0.1-, 0.2-, 1.0-, 3.0-, 6.0-, and 10.0-cm-deep wheat flour to simulate different sanitation levels that may exist in a flour mill. These rings were placed on the first and third floors of a pilot flour mill. On the first floor, temperatures inside rings with eggs reached 50 degrees C in 7-11 h only in 0.1- and 0.2-cm-deep flour treatments. In all other treatments the maximum temperatures attained generally were below 50 degrees C and inversely related to flour depth. Adults of T. castaneum on this floor were less susceptible than eggs. The egg mortality decreased linearly with an increase in flour depth, whereas that of adults decreased exponentially. All eggs and adults in rings on the third floor were killed irrespective of flour depth, because temperatures inside rings reached 50 degrees C in 15-17 h and were held above 50 degrees C for 6-8 h with the maximum temperatures ranging between 55.0 and 57.0 degrees C. Although the protective effects of flour on survival of T. castaneum eggs and adults were evident only if temperatures did not reach 50 degrees C, removal of flour accumulations is essential to improve heat treatment effectiveness.

  19. The life cycle of Gregarina cuneata in the midgut of Tribolium castaneum and the effects of parasitism on the development of insects.

    Science.gov (United States)

    Gigliolli, A A S; Julio, A H F; Conte, H

    2016-04-01

    Tribolium castaneum Herbst 1797 (Coleoptera: Tenebrionidae), an important pest of stored grains and byproducts, is naturally infected by Gregarina cuneata Stein 1848 (Apicomplexa: Gregarinidae). Changes in the life cycle of insects caused by the parasite development in the midgut were studied. Trophozoites, gamonts (solitary and associated), and gametocysts were present in the midgut of the insects. In young trophozoites, the apical region differentiated into an epimerite that firmly attached the parasite to the host epithelial cells. With maturation, trophozoites developed in gamonts that were associated with the initiation of sexual reproduction in the cell cycle, culminating in the formation of the spherical gametocyst. Morpho-functional analyses indicated that gregarines absorb nutrients from infected cells and can occlude the midgut as they develop. Consequently, nutritional depletion may interfere with the host's physiology, causing decreased growth, delayed development, and high mortality rates of the parasitized insects. These results suggest G. cuneata could be an important biological agent for controlling T. castaneum in integrated pest management programs.

  20. Insecticide activity of essential oils of Mentha longifolia, Pulicaria gnaphalodes and Achillea wilhelmsii against two stored product pests, the flour beetle, Tribolium castaneum, and the cowpea weevil, Callosobruchus maculatus.

    Science.gov (United States)

    Khani, Abbas; Asghari, Javad

    2012-01-01

    Essential oils extracted from the foliage of Mentha longifolia (L.) (Lamiales: Lamiaceae) and Pulicaria gnaphalodes Ventenat (Asterales: Asteraceae), and flowers of Achillea wilhelmsii C. Koch (Asterales: Asteraceae) were tested in the laboratory for volatile toxicity against two storedproduct insects, the flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) and the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Bruchidae). The chemical composition of the isolated oils was examined by gas chromatography-mass spectrometry. InM longifolia, the major compounds were piperitenon (43.9%), tripal (14.3%), oxathiane (9.3%), piperiton oxide (5.9%), and d-limonene (4.3%). In P. gnaphalodes, the major compounds were chrysanthenyl acetate (22.38%), 2L -4L-dihydroxy eicosane (18.5%), verbenol (16.59%), dehydroaromadendrene (12.54%), β-pinen (6.43%), and 1,8 cineol (5.6%). In A. wilhelmsii, the major compounds were 1,8 cineole (13.03%), caranol (8.26%), alpha pinene (6%), farnesyl acetate (6%), and p-cymene (6%). C maculatus was more susceptible to the tested plant products than T castaneum. The oils of the three plants displayed the same insecticidal activity against C. maculatus based on LC(50) values (between 1.54µl/L air in P. gnaphalodes, and 2.65 µl/L air in A. wilhelmsii). While the oils of A. wilhelmsii and M. longifolia showed the same strong insecticidal activity against T. castaneum (LC(50) = 10.02 and 13.05 µl/L air, respectively), the oil of P. gnaphalodes revealed poor activity against the insect (LC(50) = 297.9 µl/L air). These results suggested that essential oils from the tested plants could be used as potential control agents for stored-product insects.

  1. Efficacy of aerosol applications of methoprene and synergized pyrethrin against Tribolium castaneum adults and eggs

    Science.gov (United States)

    Experiments were performed to determine the efficacy of a single aerosol application of the insecticides methoprene and piperonyl butoxide-synergized pyrethrin, alone or in combination, and the insecticide carrier, Isopar M, against Tribolium castaneum (Herbst), the red flour beetle. The initial tes...

  2. The presence of flour affects the efficacy of aerosolized insecticides used to treat the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Toews, Michael D; Campbell, James F; Arthur, Franklin H

    2010-01-01

    Experiments were conducted in tightly sealed pilot scale warehouses to assess the efficacy of common aerosolized insecticides on all life stages of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) when exposed in dishes containing 0 to 2 g of wheat flour either under pallets or out in the open. Petri dishes containing 0, 0.1, 1, or 2 g of flour were prepared with 25 eggs, 3rd instars, pupae, or adults and then immediately treated with aerosolized solvent, Pyrethrins, or esfenvalerate. Twenty-four h after insecticide exposure, the dishes were brought to the laboratory and placed in a growth chamber and held for a 3 day moribund (knockdown) assessment and a 21 day mortality assessment. Mortality in untreated controls was generally less than 10%, with the exception of the 21 day counts of adults and eggs. Solvent-treated replications followed similar trends, except that additional mortality was observed in exposed larvae and pupae. In the insecticide-treated dishes, mortality of T. castaneum provisioned with flour generally showed a linear decrease with increasing flour deposits. Regardless of life stage, mortality did not exceed 60% when individuals were exposed in petri dishes containing 2 g of flour. Exposure location also made a significant difference in observed mortality. While mortality never exceeded 75% in dishes positioned under pallets, there was never less than 80% mortality in dishes exposed in the open. Although there was a perceptible increase in mortality with esfenvalerate compared to Pyrethrins, these differences were considerably less than the variation observed among flour deposits. The study suggests that sanitation and preparation prior to aerosol insecticide treatments were more important than choice of a particular insecticide. PMID:21268701

  3. Effects of flour and milling debris on efficacy of beta-cyfluthrin for control of Tribolium castaneum

    Science.gov (United States)

    Laboratory and field studies were conducted to determine if accumulation of a flour food source or milling debris affected residual efficacy of beta-cyfluthrin for control of Tribolium castaneum (Herbst), the red flour beetle. In the laboratory study, the high label rate of 20 mg active ingredient (...

  4. Food source provisioning and susceptibility of immature and adult Tribolium castaneum on concrete partially treated with chlorfenapyr (Phantom®)

    Science.gov (United States)

    A series of experiments were conducted in which adults, pupae, and 4-week-old larvae of Tribolium castaneum (Herbst), the red flour beetle, were exposed separately on concrete arenas partially treated (14.4 % of the total area) with the insecticide chlorfenapyr (Phantom®) at 1.1 g active ingredient/...

  5. Herbst treatment in late adolescence

    DEFF Research Database (Denmark)

    Bakke, Merete; Paulsen, H U

    1989-01-01

    during retention necessitated retreatment. Treatment resulted in marked dentoalveolar changes, increase in mandibular length and ramus height, apposition and remodelling of condylar heads and flattening of the temporal joint surface. After treatment masseter strength was 40 per cent reduced, probably due......In an almost full-grown male of 17 years, functional and morphological changes were followed during Herbst treatment and subsequent control. Retrognathism of the mandible was overcorrected, and then normalized by reverse headgear to the maxilla. Partial relapse due to insufficient cooperation...

  6. Residual efficacy of aerosols to control Tribolium castaneum and Tribolium confusum

    OpenAIRE

    Arthur, F. H

    2010-01-01

    Aerosol insecticides can be important components of insect management plans for mills, food warehouses, and processing plants. In the United States, synergized pyrethrin is used alone or combined with an insect growth regulator (IGR), either methoprene or hydroprene. The presence of food material can result in increased survival of adult Tribolium castaneum (Herbst) or Tribolium confusum Jacquelin du Val exposed to synergized pyrethrins, but larvae appear to be more susceptible than adults. R...

  7. 不同地区转Bt基因抗虫水稻稻谷对赤拟谷盗生长发育的影响%Influence of transgenic Bt rice on the growth and development of Tribolium castaneum (Coleoptera : Tenebrionidae)

    Institute of Scientific and Technical Information of China (English)

    姚英娟; 徐雪亮; 严寒; 蔡万伦; 林拥军; 华红霞

    2012-01-01

    The effect of the transgenic Bt rice Shanyou 63 with crylAb/crylAc, cry2A and crylC (TT51, T2A-1 and T1C-19) on the growth and development of Tribolium castaneum Herbst was investigated using rice from three different areas (Wuxue, Xiaogan and Suizhou in Hubei Province). The results show that the duration of the egg, larval, pupal and preoviposition stages were 3 to 4 days, 22 to 27 days, 6 to 7 days and 5 to 7 days, respectively. Hatchability, pupation rate and emergence rate were 85% to 100% , 85% to 97% and 82% to 97% , respectively. The weight of 100 pupae was 0. 25 to 0. 31 g and the sex ratio was 0. 8 to 1. 4. No significant differences were found among the different rice varieties. All three Bt proteins could be detected in T. castaneum larvae, but the accumulated amount was very small. There was no significant accumulative effect despite larvae being reared on these strains.%为了明确来自湖北武穴、孝感、随州3个不同地区的转cry1Ab/cry1Ac、cry2A、cry1C基因明恢63(分别命名为TT51、T2A-1和T1C-19)对赤拟谷盗Tribolium castaneum Herbst生长发育的影响,在室内以不同的转Bt基因稻谷继代饲养赤拟谷盗6代,结果表明:各处理赤拟谷盗的卵期3~4d,卵孵化率85% ~ 100%,幼虫期22 ~ 27 d,化蛹率85%~97%,蛹期6~7d,百蛹重0.25~0.31 g,性比0.8~1.4,羽化率82%~97%,产卵前期5~7d.不同抗虫转基因水稻对赤拟谷盗各发育历期及生命表参数没有显著的影响,同一转基因事件,没有因为种植地区的不同造成对赤拟谷盗生长发育的差异.3种Bt蛋白在继代饲养的赤拟谷盗幼虫体内检测均呈阳性反应,但积累量很小,继代饲养后,没有发现在赤拟谷盗体内明显的累积.

  8. Effects of microwave radiation and conductive heating on Tribolium castaneum microstructure.

    Science.gov (United States)

    Lu, H H; Zhou, J C; Yan, D; Zhao, S M; Xiong, S B

    2011-01-01

    Microwave radiation and conductive heating were used to completely kill adult Tribolium castaneum (Coleoptera: Tenebrionidae) in wheat flour to protect the flour during storage without significantly effecting its quality. The microstructure of T. castaneum was analyzed to reveal the mechanisms leading to death under microwave and heat treatments. Microwave radiation and conductive heating had different effects on the microstructure of the cuticle of adult T. castaneum and on the ultrastructure of the cells of the epidermis, fat body, and midgut. Both treatments caused a large cavity to appear in the nucleus and the disappearance of mitochondria and the Golgi apparatus. After microwave treatment, there was little change in the surface microstructure but the epidermis was of uneven thickness and the four outer layers of the cuticle were thinner. Nuclear size was essentially unchanged, but fat body cells were fewer and coalesced together. In contrast, conductive heating led to a disordered arrangement of cells on the surface of T. castaneum and indistinct boundaries between layers of the cuticle. The nuclei were enlarged and the fat body cells noticeably fewer and indistinct with a scattered distribution. Thus, microwave treatment produced less severe effects on the surface microstructure and cellular ultrastructure of T. castaneum than did conductive heating. It is concluded that these cellular and surface changes were responsible for the death of T. castaneum.

  9. Efficacy of aerosol applications of methoprene and synergized pyrethrin against Tribolium castaneum adults and eggs.

    Science.gov (United States)

    Tucker, Angela M; Campbell, James F; Arthur, Frank H; Zhu, Kun Yan

    2014-06-01

    Experiments were performed to determine the efficacy of a single aerosol application of the insecticides methoprene and piperonyl butoxide-synergized pyrethrin, alone or in combination, and the insecticide carrier, Isopar M, against Tribolium castaneum (Herbst), the red flour beetle. The initial test exposed adults to insecticide treatments and placed male/female pairs in flour. All adults exposed to synergized pyrethrin were knocked down for at least 24 h after exposure but they recovered. High adult survival and similar average numbers of living F1 progeny were produced regardless of treatment exposure. In a separate test, insecticide treatments were directly applied to newly laid eggs, which resulted in the suppression of egg hatch. Synergized pyrethrin was the most effective insecticide (P castaneum egg hatch and development to the adult stage.

  10. 赤拟谷盗HSP70基因克隆和竞争定量PCR检测%Cloning and quantitative competitive PCR assay of HSP70 gene in Tribolium castaneum ( Coleoptera: Tenebrionidae)

    Institute of Scientific and Technical Information of China (English)

    苏丽娟; 董晓慧; 尹新明

    2011-01-01

    To study the expression changes of heat shock protein 70 (a highly conserved protein) in Tribolium castaneum after exposure to heat stress, a fragment of 681 bp encoding for HSP70 was amplified and sequenced from T.castaneum.The fragment encoded 227 amino acid residues with the GenBank accession no.HM345948.The result of homology analysis showed that this fragment shared 97% identity with hsp70 from L.decemlineata (AF322911.1 ).Comparison of the deduced amino acid sequences of HSP70 in T.castaneum with those in Leptinotarsa decemlineata, Mamestra brassicae, Drosophila melanogaster and Liriomyza sativae indicated that they shared more than 94% identity.The internal competitor used in quantitative competitive PCR was obtained by RT-PCR.The detection system of hsp70 was constructed by PCR amplification using the same quantity of target cDNA and a series of diluted internal competitor as template.The linear equation of standard curve was Y= 1.032X- 1.618 (r2 =0.975).This study provides a very convenient method for the quantitation of the hsp70 expression changes in T.castaneum and offers the basic data for the prevention and control of pests using thermal control technology.%为研究赤拟谷盗Tribolium castaneum受到热胁迫后高度保守的热激蛋白70(heat shock protein 70,HSP70)基因的表达变化,本研究扩增了681 bp的赤拟谷盗hsp70片段,编码227个氨基酸残基,GenBank登录号为HM345948.同源性分析表明:赤拟谷盗hsp70核苷酸序列与马铃薯甲虫Leptinotarsa decemlineata的hsp70(GenBank登录号:AF322911.1)同源性最高,为97%;其推测的蛋白序列与马铃薯甲虫、甘蓝夜蛾Mamestra brassicae、黑腹果蝇Drosophila melanogaster和美洲斑潜蝇Liriomyza,sativae的HSP70蛋白均有94%以上的同源性.利用RT-PCR技术得到与赤拟谷盗hsp70进行竞争定量的内部竞争物,以等量的目标cDNA和一系列稀释的竞争模板进行竞争PCR扩增,构建了hsp70的竞争定量PCR检测体系,该

  11. A genome-wide inventory of neurohormone GPCRs in the red flour beetle Tribolium castaneum

    DEFF Research Database (Denmark)

    Hauser, Frank; Cazzamali, Giuseppe; Williamson, Michael;

    2008-01-01

    projects has enabled us to obtain a complete inventory of neurohormone GPCRs in these insects and, by a comparative genomics approach, to analyze the evolution of these proteins. The red flour beetle Tribolium castaneum is the latest addition to the list of insects with a sequenced genome and the first...... consumption. In addition, T. castaneum is a model for insect development. Here, we have investigated the presence of neurohormone GPCRs in Tribolium and compared them with those from the fruit fly Drosophila melanogaster (Diptera) and the honey bee Apis mellifera (Hymenoptera). We found 20 biogenic amine...... coleopteran (beetle) to be sequenced. Coleoptera is the largest insect order and about 30% of all animal species living on earth are coleopterans. Some coleopterans are severe agricultural pests, which is also true for T. castaneum, a global pest for stored grain and other dried commodities for human...

  12. Behavioral responses of plum curculio (Coleoptera: Curculionidae) to different enantiomer concentrations and blends of the synthetic aggregation pheromone grandisoic acid

    Science.gov (United States)

    Host plant odors are important for insect location of food and mates. Synergy between host plant odors and aggregation pheromones occurs in many Curculionidae species. The plum curculio Conotrachelus nenuphar Herbst (Coleoptera: Curculionidae) is a major pest of pome and stone fruit. Males produce t...

  13. Aparelho Herbst: protocolos de tratamento precoce e tardio The Herbst appliance: early and late treatment protocol

    Directory of Open Access Journals (Sweden)

    Omar Gabriel da Silva Filho

    2005-02-01

    Full Text Available Até que ponto o crescimento mandibular pode ser realmente influenciado pelos aparelhos ortopédicos de reposicionamento anterior, ainda permanece indecifrável. Mas a lógica leva a crer que se existe alguma chance de potencializar o deslocamento anterior da mandíbula, ela será mais certa quando se utiliza aparelhos de avanço contínuo. O presente artigo discorre sobre os protocolos de tratamento precoce e tardio para a correção da deficiência mandibular com o aparelho Herbst.The question as to what extent mandibular growth may be influenced by orthopedic appliances remains unanswered. However, it seems reasonable to believe that the use of continuous forces potentialize the anterior displacement of the mandible. The current paper describes the early and late protocols of treatment of the mandibular deficiency with the Herbst appliance.

  14. Next generation sequencing based transcriptome analysis of septic-injury responsive genes in the beetle Tribolium castaneum.

    Science.gov (United States)

    Altincicek, Boran; Elashry, Abdelnaser; Guz, Nurper; Grundler, Florian M W; Vilcinskas, Andreas; Dehne, Heinz-Wilhelm

    2013-01-01

    Beetles (Coleoptera) are the most diverse animal group on earth and interact with numerous symbiotic or pathogenic microbes in their environments. The red flour beetle Tribolium castaneum is a genetically tractable model beetle species and its whole genome sequence has recently been determined. To advance our understanding of the molecular basis of beetle immunity here we analyzed the whole transcriptome of T. castaneum by high-throughput next generation sequencing technology. Here, we demonstrate that the Illumina/Solexa sequencing approach of cDNA samples from T. castaneum including over 9.7 million reads with 72 base pairs (bp) length (approximately 700 million bp sequence information with about 30× transcriptome coverage) confirms the expression of most predicted genes and enabled subsequent qualitative and quantitative transcriptome analysis. This approach recapitulates our recent quantitative real-time PCR studies of immune-challenged and naïve T. castaneum beetles, validating our approach. Furthermore, this sequencing analysis resulted in the identification of 73 differentially expressed genes upon immune-challenge with statistical significance by comparing expression data to calculated values derived by fitting to generalized linear models. We identified up regulation of diverse immune-related genes (e.g. Toll receptor, serine proteinases, DOPA decarboxylase and thaumatin) and of numerous genes encoding proteins with yet unknown functions. Of note, septic-injury resulted also in the elevated expression of genes encoding heat-shock proteins or cytochrome P450s supporting the view that there is crosstalk between immune and stress responses in T. castaneum. The present study provides a first comprehensive overview of septic-injury responsive genes in T. castaneum beetles. Identified genes advance our understanding of T. castaneum specific gene expression alteration upon immune-challenge in particular and may help to understand beetle immunity in general.

  15. First evidence of DNA methylation in insect Tribolium castaneum: environmental regulation of DNA methylation within heterochromatin.

    Science.gov (United States)

    Feliciello, Isidoro; Parazajder, Josip; Akrap, Ivana; Ugarković, Durđica

    2013-05-01

    DNA methylation has been studied in many eukaryotic organisms, in particular vertebrates, and was implicated in developmental and phenotypic variations. Little is known about the role of DNA methylation in invertebrates, although insects are considered as excellent models for studying the evolution of DNA methylation. In the red flour beetle, Tribolium castaneum (Tenebrionidae, Coleoptera), no evidence of DNA methylation has been found till now. In this paper, a cytosine methylation in Tribolium castaneum embryos was detected by methylation sensitive restriction endonucleases and immuno-dot blot assay. DNA methylation in embryos is followed by a global demethylation in larvae, pupae and adults. DNA demethylation seems to proceed actively through 5-hydroxymethylcytosine, most probably by the action of TET enzyme. Bisulfite sequencing of a highly abundant satellite DNA located in pericentromeric heterochromatin revealed similar profile of cytosine methylation in adults and embryos. Cytosine methylation was not only restricted to CpG sites but was found at CpA, CpT and CpC sites. In addition, complete cytosine demethylation of heterochromatic satellite DNA was induced by heat stress. The results reveal existence of DNA methylation cycling in T. castaneum ranging from strong overall cytosine methylation in embryos to a weak DNA methylation in other developmental stages. Nevertheless, DNA methylation is preserved within heterochromatin during development, indicating its role in heterochromatin formation and maintenance. It is, however, strongly affected by heat stress, suggesting a role for DNA methylation in heterochromatin structure modulation during heat stress response.

  16. iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum.

    Science.gov (United States)

    Dönitz, Jürgen; Schmitt-Engel, Christian; Grossmann, Daniela; Gerischer, Lizzy; Tech, Maike; Schoppmeier, Michael; Klingler, Martin; Bucher, Gregor

    2015-01-01

    The iBeetle-Base (http://ibeetle-base.uni-goettingen.de) makes available annotations of RNAi phenotypes, which were gathered in a large scale RNAi screen in the red flour beetle Tribolium castaneum (iBeetle screen). In addition, it provides access to sequence information and links for all Tribolium castaneum genes. The iBeetle-Base contains the annotations of phenotypes of several thousands of genes knocked down during embryonic and metamorphic epidermis and muscle development in addition to phenotypes linked to oogenesis and stink gland biology. The phenotypes are described according to the EQM (entity, quality, modifier) system using controlled vocabularies and the Tribolium morphological ontology (TrOn). Furthermore, images linked to the respective annotations are provided. The data are searchable either for specific phenotypes using a complex 'search for morphological defects' or a 'quick search' for gene names and IDs. The red flour beetle Tribolium castaneum has become an important model system for insect functional genetics and is a representative of the most species rich taxon, the Coleoptera, which comprise several devastating pests. It is used for studying insect typical development, the evolution of development and for research on metabolism and pest control. Besides Drosophila, Tribolium is the first insect model organism where large scale unbiased screens have been performed.

  17. Malpighian tubule development in the red flour beetle (Tribolium castaneum).

    Science.gov (United States)

    King, Benedict; Denholm, Barry

    2014-11-01

    Malpighian tubules (MpTs) are the major organ for excretion and osmoregulation in most insects. MpT development is characterised for Drosophila melanogaster, but not other species. We therefore do not know the extent to which the MpT developmental programme is conserved across insects. To redress this we provide a comprehensive description of MpT development in the beetle Tribolium castaneum (Coleoptera), a species separated from Drosophila by >315 million years. We identify similarities with Drosophila MpT development including: 1) the onset of morphological development, beginning when tubules bud from the gut and proliferate to increase organ size. 2) the tubule is shaped by convergent-extension movements and oriented cell divisions. 3) differentiated tip cells activate EGF-signalling in distal MpT cells through the ligand Spitz. 4) MpTs contain two main cell types - principal and stellate cells, differing in morphology and gene expression. We also describe development of the beetle cryptonephridial system, an adaptation for water conservation, which represents a major modification of the MpT ground plan characterised by intimate association between MpTs and rectum. This work establishes a new model to compare MpT development across insects, and provides a framework to help understand how an evolutionary novelty - the cryptonephridial system - arose during organ evolution.

  18. Nuclear structures in Tribolium castaneum oocytes.

    Science.gov (United States)

    Bogolyubov, Dmitry S; Batalova, Florina M; Kiselyov, Artyom M; Stepanova, Irina S

    2013-10-01

    The first ultrastructural and immunomorphological characteristics of the karyosphere (karyosome) and extrachromosomal nuclear bodies in the red flour beetle, Tribolium castaneum, are presented. The karyosphere forms early in the diplotene stage of meiotic prophase by the gathering of all oocyte chromosomes in a limited nuclear volume. Using the BrUTP assay, T. castaneum oocyte chromosomes united in the karyosphere maintain their transcriptional activity until the end of oocyte growth. Hyperphosphorylated RNA polymerase II and basal transcription factors (TFIID and TFIIH) were detected in the perichromatin region of the karyosphere. The T. castaneum karyosphere has an extrachromosomal capsule that separates chromosomes from the rest of the nucleoplasm. Certain structural proteins (F-actin, lamin B) were found in the capsule. Unexpectedly, the karyosphere capsule in T. castaneum oocytes was found to be enriched in TMG-capped snRNAs, which suggests that the capsule is not only a structural support for the karyosphere, but may be involved in biogenesis of snRNPs. We also identified the counterparts of 'universal' extrachromosomal nuclear domains, Cajal bodies (CBs) and interchromatin granule clusters (IGCs). Nuclear bodies containing IGC marker protein SC35 display some features unusual for typical IGCs. SC35 domains in T. castaneum oocytes are predominantly fibrillar complex bodies that do not contain trimethyl guanosine (TMG)-capped small nuclear (sn) RNAs. Microinjections of 2'-O-methyl (U)22 probes into the oocytes allowed revealing poly(A)+ RNAs in these nuclear domains. Several proteins related to mRNA export (heterogeneous ribonucleoprotein core protein A1, export adapters Y14 and Aly and export receptor NXF1) were also detected there. We believe that unusual SC35 nuclear domains of T. castaneum oocytes are possibly involved in mRNP but not snRNP biogenesis.

  19. Response of body size and developmental time of Tribolium castaneum to constant versus fluctuating thermal conditions.

    Science.gov (United States)

    Małek, D; Drobniak, S; Gozdek, A; Pawlik, K; Kramarz, P

    2015-07-01

    Temperature has profound effects on biological functions at all levels of organization. In ectotherms, body size is usually negatively correlated with ambient temperature during development, a phenomenon known as The Temperature-Size Rule (TSR). However, a growing number of studies have indicated that temperature fluctuations have a large influence on life history traits and the implications of such fluctuations for the TSR are unknown. Our study investigated the effect of different constant and fluctuating temperatures on the body mass and development time of red flour beetles (Tribolium castaneum Herbst, 1797); we also examined whether the sexes differed in their responses to thermal conditions. We exposed the progeny of half-sib families of a T. castaneum laboratory strain to one of four temperature regimes: constant 30°C, constant 25°C, fluctuating with a daily mean of 30°C, or fluctuating with a daily mean of 25°C. Sex-specific development time and body mass at emergence were determined. Beetles developed the fastest and had the greatest body mass upon emergence when they were exposed to a constant temperature of 30°C. This pattern was reversed when beetles experienced a constant temperature of 25°C: slowest development and lowest body mass upon emergence were observed. Fluctuations changed those effects significantly - impact of temperature on development time was smaller, while differences in body mass disappeared completely. Our results do not fit TSR predictions. Furthermore, regardless of the temperature regime, females acquired more mass, while there were no differences between sexes in development time to eclosion. This finding fails to support one of the explanations for smaller male size: that selection favors the early emergence of males. We found no evidence of genotype × environment interactions for selected set of traits.

  20. Identification and evolution of two insulin receptor genes involved in Tribolium castaneum development and reproduction.

    Science.gov (United States)

    Sang, Ming; Li, Chengjun; Wu, Wei; Li, Bin

    2016-07-10

    The insulin and insulin-like signaling (IIS) pathway exists in a wide range of organisms from mammals to invertebrates and regulates several vital physiological functions. A phylogenetic analysis have indicated that insulin receptors have been duplicated at least twice among vertebrates, whereas only one duplication occurred in insects before the differentiation of Coleoptera, Hymenoptera, and Hemiptera. Thus, we cloned two putative insulin receptor genes, T.cas-ir1 and T.cas-ir2, from T. castaneum and determined that T.cas-ir1 is most strongly expressed during the late adult and early pupal stages, whereas T.cas-ir2 is most strongly expressed during the late larval stage. We found that larval RNAi against T.cas-ir1 and T.cas-ir2 causes 100% and 42.0% insect death, respectively, and that parental RNAi against T.cas-ir1 and T.cas-ir2 leads to 100% and 33.3% reductions in beetle fecundity, respectively. The hatching rate of ds-ir2 insects was 66.2%. Moreover, RNAi against these two genes increased the expression of the pkc, foxo, jnk, cdc42, ikk, and mekk genes but decreased erk gene expression. Despite these similarities, these two genes act via distinct regulatory pathways. These results indicate that these two receptors have functionally diverged with respect to the development and reproduction of T. castaneum, even though they retain some common regulatory signaling pathways.

  1. Comparative toxicity and micronuclei formation in Tribolium castaneum, Callosobruchus maculatus and Sitophilus oryzae exposed to high doses of gamma radiation.

    Science.gov (United States)

    Ahmadi, Mehrdad; Mozdarani, Hossein; Abd-Alla, Adly M M

    2015-07-01

    The effects of gamma radiation on mortality and micronucleus formation in Tribolium castaneum Herbst, Callosobruchus maculatus (F.) and Sitophilus oryzae (L.) genital cells were evaluated. Two groups of healthy and active adult insects 1-3 and 8-10 days old were irradiated with various doses (50-200 Gy) gamma ray. Seven days post-irradiation; mortality rates and micronucleus formation were assessed in genital cells of the irradiated insects. The results show that with increasing gamma doses, the mortality rate of each species increased and T. castaneum and S. oryzae showed the low and high sensitivity respectively. It was shown that the micronucleus appearance in the tested insects had correlation with amount and intensity of radiation doses. Moreover our results indicate different levels in the genotoxicity of gamma radiation among the insects' genital cells under study. The frequency of micronuclei in genital cells of 1-3 days old insects exposed to 50 and 200 Gy were 12.6 and 38.8 Mn/1000 cells in T. castaneum, 20.8 and 46.8 Mn/1000 cells in C. maculatus and 16.8 and 57.2 Mn/1000 cells in S. oryzae respectively. A high sensitivity of the genital cells to irradiation exposure was seen in S. oryzae correlated with its high mortality rate compared with the other two species. These results might be indicative of inflicting chromosomal damage expressed as micronucleus in high mortality rates observed in the pest population; an indication of genotoxic effects of radiation on the studied species. PMID:25898238

  2. Comparative toxicity and micronuclei formation in Tribolium castaneum, Callosobruchus maculatus and Sitophilus oryzae exposed to high doses of gamma radiation.

    Science.gov (United States)

    Ahmadi, Mehrdad; Mozdarani, Hossein; Abd-Alla, Adly M M

    2015-07-01

    The effects of gamma radiation on mortality and micronucleus formation in Tribolium castaneum Herbst, Callosobruchus maculatus (F.) and Sitophilus oryzae (L.) genital cells were evaluated. Two groups of healthy and active adult insects 1-3 and 8-10 days old were irradiated with various doses (50-200 Gy) gamma ray. Seven days post-irradiation; mortality rates and micronucleus formation were assessed in genital cells of the irradiated insects. The results show that with increasing gamma doses, the mortality rate of each species increased and T. castaneum and S. oryzae showed the low and high sensitivity respectively. It was shown that the micronucleus appearance in the tested insects had correlation with amount and intensity of radiation doses. Moreover our results indicate different levels in the genotoxicity of gamma radiation among the insects' genital cells under study. The frequency of micronuclei in genital cells of 1-3 days old insects exposed to 50 and 200 Gy were 12.6 and 38.8 Mn/1000 cells in T. castaneum, 20.8 and 46.8 Mn/1000 cells in C. maculatus and 16.8 and 57.2 Mn/1000 cells in S. oryzae respectively. A high sensitivity of the genital cells to irradiation exposure was seen in S. oryzae correlated with its high mortality rate compared with the other two species. These results might be indicative of inflicting chromosomal damage expressed as micronucleus in high mortality rates observed in the pest population; an indication of genotoxic effects of radiation on the studied species.

  3. [Karyosphere capsule in Tribolium castaneum oocytes].

    Science.gov (United States)

    Batalova, F M; Bogoliubov, D S

    2013-01-01

    Structure and composition of the karyosphere (karyosome) capsule were studied in the oocytes of a laboratory insect, Tribolium castaneum, with the use of electron microscopy and immunoelectron cytochemistry. Basing on the study of nuclear structure dynamics, we distinguished 8 stages that characterize the period of oocyte growth. At the diplotene stage, T. castaneum oocyte chromosomes conjoin early into a compact karyosphere, but a significant chromatin condensation does not occur. The process of karyosphere formation is accompanied by the development of an extensive extrachromosome capsule surrounding chromatin. The capsule consists of a material of different morphological types. Significant molecular components of the T. castaneum karyosphere capsule are represented by the proteins of nuclear matrix including F-actin and lamin B. Besides the structural proteins, the Sm proteins of small nuclear (sn) RNPs and mature 2,2,7-trimethyl guanosine (TMG) 5'-capped snRNAs are revealed immunocytochemically in the karyosphere capsule. The obtained data can form a basis for further expansion of ideas on the functions of the karyosphere capsule as a specialized extrachromosomal nuclear domain of the oocytes. We believe that the T. castaneum karyosphere capsule plays not only a structural role, but may be involved directly in the processes related to gene expression.

  4. 电子束辐照对赤拟谷盗脂肪酸值的影响%FATTY ACID ANALYSIS ON ADULT OF TRIBOLIUM CASTANEURM(HERBST) IRRADIATED BY ELECTRON BEAM

    Institute of Scientific and Technical Information of China (English)

    王晶磊

    2015-01-01

    采用电子束辐照处理赤拟谷盗Tribolium castaneum (Herbst)成虫,研究在辐照剂量分别为120 Gy、180 Gy、300 Gy、420 Gy、500 Gy、1200 Gy和1800 Gy的7个处理后虫体内脂肪酸及相对含量变化.通过GC-MS分析结果表明,赤拟谷盗成虫体内的脂肪酸为肉豆蔻酸、棕榈酸、油酸,受电子束辐照后赤拟谷盗成虫脂肪酸种类都未出现变化,但相对含量变化明显.

  5. 电子束对赤拟谷盗成虫辐照效应研究%Fatty Acid Analysis on Adult of Tribolium castaneum Irradiated by Electron Beam

    Institute of Scientific and Technical Information of China (English)

    王晶磊; 王殿轩; 徐威

    2012-01-01

      采用电子束辐照处理赤拟谷盗Tribolium castaneum(Herbst)成虫,研究在辐照剂量分别为120、180、300、420、500和1200 Gy的6个处理后虫体内脂肪酸种类及相对含量变化。通过GC-MS分析结果表明,赤拟谷盗成虫体内的脂肪酸主要为肉豆蔻酸、棕榈酸、油酸、硬脂酸。结果表明:受电子束辐照后赤拟谷盗成虫脂肪酸种类都未出现变化,但相对含量变化明显。%  Fatty acid analysis on adults of Tribolium castaneurn(Herbst) was carried out with GC-MS method after electron beam irradiation in the dosage of 0,120,240,300,420,500 and 1 200 Gy,respectively.The results indicate that the fat acid from Herbst included in nutmeg acid,palmitic acid,oleic acid and stearic acid.Under the condition of high dosage of irradiation electron beam,there is a significant influence on the content of fatty acid of S.oryzae adults,but not for the kinds of fat acids.

  6. Treatment of Class II high angle malocclusions with the Herbst appliance: a cephalometric investigation.

    Science.gov (United States)

    Schiavoni, R; Grenga, V; Macri, V

    1992-11-01

    The purpose of this study was to examine the results of treatment of Class II malocclusions by using two different designs of the Herbst appliance. Cephalometric records from lateral headplates of 19 consecutively treated Class II cases were evaluated. The headplates were taken before and after the treatment stage in which the Herbst appliance was used. The patients were divided into two groups: the first group, normohypodivergent, was treated with the Herbst appliance attached to bands; the second group, hyperdivergent, was treated with the Herbst appliance attached to acrylic splints in which a high-pull headgear was also used. The results were compared between these groups and with a control group age-matched from Bolton standards to match the changes in the Herbst samples against what might be expected in case of normal growth during similar periods of time. The results of the investigation revealed the following: (1) 9 months of treatment resulted in Class I dental arch relationships in all 19 cases; (2) the Herbst appliance attached to bands did not significantly modify the vertical growth pattern of the normohypodivergent patients; and (3) in hyperdivergent patients, the use of a Herbst appliance attached to acrylic splints in conjunction with the use of a high-pull headgear allowed a better control of the vertical dimension, as assessed by the cephalometric parameters (FA, FMA, Go-Gn-SN). The clinician should be aware of the different dentofacial changes induced in the vertical plane by different designs of the Herbst appliance to better program treatment strategy.

  7. Egg morphology and chorionic ultrastructure of key stored product insect pests of the United States

    Science.gov (United States)

    Eggs of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) were imaged with scanning electron microscopy to explore how respiratory openings on the chorion surface may be related to the efficacy of fumigants. Each P. interpunctella eg...

  8. Cone beam computed tomography study of apical root resorption induced by Herbst appliance

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    João Paulo SCHWARTZ

    2015-10-01

    Full Text Available Objective This study evaluated the frequency of root resorption during the orthodontic treatment with Herbst appliance by Cone Beam Computed Tomography (CBCT.Material and Methods The sample comprised 23 patients (11 men, 12 women; mean ages 15.76±1.75 years with Class II division 1 malocclusion, treated with Herbst appliance. CBCT was obtained before treatment (T0 and after Herbst treatment (T1. All the dental roots, except third molars, were evaluated, and apical root resorption was determined using the axial guided navigation method. Paired t-tests and Wilcoxon T Test were used to compare the dependent samples in parametric and nonparametric cases, respectively. Chi-Square Test with Yates’ correction was used to evaluate the relationship between apical root resorption and gender. Results were considered at a significance level of 5%.Results Apical resorption was detected by CBCT in 57.96% of 980 roots that underwent Herbst appliance treatment. All patients had minimal resorption and there was no statistical significance between the genders.Conclusion CBCT three-dimensional evaluation showed association between Herbst appliance and minimal apical root resorption, mostly in the anchoring teeth, without clinical significance.

  9. Cone beam computed tomography study of apical root resorption induced by Herbst appliance

    Science.gov (United States)

    SCHWARTZ, João Paulo; RAVELI, Taísa Boamorte; ALMEIDA, Kélei Cristina de Mathias; SCHWARTZ-FILHO, Humberto Osvaldo; RAVELI, Dirceu Barnabé

    2015-01-01

    Objective This study evaluated the frequency of root resorption during the orthodontic treatment with Herbst appliance by Cone Beam Computed Tomography (CBCT). Material and Methods The sample comprised 23 patients (11 men, 12 women; mean ages 15.76±1.75 years) with Class II division 1 malocclusion, treated with Herbst appliance. CBCT was obtained before treatment (T0) and after Herbst treatment (T1). All the dental roots, except third molars, were evaluated, and apical root resorption was determined using the axial guided navigation method. Paired t-tests and Wilcoxon T Test were used to compare the dependent samples in parametric and nonparametric cases, respectively. Chi-Square Test with Yates’ correction was used to evaluate the relationship between apical root resorption and gender. Results were considered at a significance level of 5%. Results Apical resorption was detected by CBCT in 57.96% of 980 roots that underwent Herbst appliance treatment. All patients had minimal resorption and there was no statistical significance between the genders. Conclusion CBCT three-dimensional evaluation showed association between Herbst appliance and minimal apical root resorption, mostly in the anchoring teeth, without clinical significance. PMID:26537718

  10. The effects, limitations, and long-term dentofacial adaptations to treatment with the Herbst appliance.

    Science.gov (United States)

    Pancherz, H

    1997-12-01

    The purpose of this article is to summarize the existing scientific data with respect to the short- and long-term effects of the Herbst appliance on the occlusion and on the maxillo/mandibular complex. The article also discusses the treatment indications and possible treatment limitations. The Herbst method is most effective in the treatment of Class II malocclusions. Long-term stability seems to be dependent on a stable cuspal interdigitation. Marked mandibular morphological changes occur during therapy and sagittal condylar growth is increased. Posttreatment, most of the mandibular morphological changes revert and no long-term influence of Herbst treatment on mandibular growth can be verified. The appliance effect on the maxillary complex can be compared with that of a high-pull headgear. Without proper retention, however, this effect is of a temporary nature. Herbst treatment is especially indicated in the permanent dentition at or just after the pubertal peak of growth. Mixed dentition treatment is not recommended, as a stable cuspal interdigitation after therapy is difficult to achieve and relapses are prone to occur. In the nongrowing patient, the appliance should be used with great caution.

  11. Changes in alveolar bone support induced by the Herbst appliance: a tomographic evaluation

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    João Paulo Schwartz

    2016-04-01

    Full Text Available ABSTRACT Objective: This study evaluated alveolar bone loss around mandibular incisors, induced by the Herbst appliance. Methods: The sample consisted of 23 patients (11 men, 12 women; mean age of 15.76 ± 1.75 years, Class II, Division 1 malocclusion, treated with the Herbst appliance. CBCT scans were obtained before treatment (T0 and after Herbst treatment (T1. Vertical alveolar bone level and alveolar bone thickness of mandibular incisors were assessed. Buccal (B, lingual (L and total (T bone thicknesses were assessed at crestal (1, midroot (2 and apical (3 levels of mandibular incisors. Student's t-test and Wilcoxon t-test were used to compare dependent samples in parametric and nonparametric cases, respectively. Pearson's and Spearman's rank correlation analyses were performed to determine the relationship of changes in alveolar bone thickness. Results were considered at a significance level of 5%. Results: Mandibular incisors showed no statistical significance for vertical alveolar bone level. Alveolar bone thickness of mandibular incisors significantly reduced after treatment at B1, B2, B3, T1 and significantly increased at L2. The magnitude of the statistically significant changes was less than 0.2 mm. The changes in alveolar bone thickness showed no statistical significance with incisor inclination degree. Conclusions: CBCT scans showed an association between the Herbst appliance and alveolar bone loss on the buccal surface of mandibular incisors; however, without clinical significance.

  12. Functional value of elytra under various stresses in the red flour beetle, Tribolium castaneum

    Science.gov (United States)

    Linz, David M.; Hu, Alan W.; Sitvarin, Michael I.; Tomoyasu, Yoshinori

    2016-01-01

    Coleoptera (beetles) is a massively successful order of insects, distinguished by their evolutionarily modified forewings called elytra. These structures are often presumed to have been a major driving force for the successful radiation of this taxon, by providing beetles with protection against a variety of harsh environmental factors. However, few studies have directly demonstrated the functional significance of the elytra against diverse environmental challenges. Here, we sought to empirically test the function of the elytra using Tribolium castaneum (the red flour beetle) as a model. We tested four categories of stress on the beetles: physical damage to hindwings, predation, desiccation, and cold shock. We found that, in all categories, the presence of elytra conferred a significant advantage compared to those beetles with their elytra experimentally removed. This work provides compelling quantitative evidence supporting the importance of beetle forewings in tolerating a variety of environmental stresses, and gives insight into how the evolution of elytra have facilitated the remarkable success of beetle radiation. PMID:27708390

  13. Ionizing radiation control of Tribolium castaneum in wheat flour type 000

    International Nuclear Information System (INIS)

    The insects, mainly those of the coleoptera order, produce serious changes on the grains and flours, producing in some regions up to 50 % loss. Taking in account the information available up to date, this experiment consists of putting under the effect of the ionizing radiation specimens of Tribolium castaneum feeded with bread flour type 000, with the purpose of controling their biological cycle. They received gamma radiation doses between 250 and 2000 Gy, using 60Co source. The daily observation made over a population of 590 insects, indicates the efficiency of the procedure, non toxic, which provokes the sterility at 250 Gy and inmediate dead starting at 1750 Gy. On the other hand, it was verified that the DL50 on the insects irradiated at the lower of eight different doses applied, reaches 15,3 days, against the 162,6 days of the reference Tribolium. Then it is concluded that it is technologically feasible the application of ionizing radiation to the bread wheat flour type 000 for controling this main plage. (Author)

  14. Walking stability of Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrichidae).

    Science.gov (United States)

    Pires, E M; Nogueira, R M; Pina, D S; Manica, C L M; Faroni, L R A; Moreira, P S A

    2016-04-19

    Results obtained in studies can contribute to the advancement of science and innovative methods and techniques for developing practical activities. Reporting conditions that may restrict the implementation of research is critical to ensure the optimal development of further technical studies. The objective of this study was to assess the walking stability of R. dominica on a flat and smooth surface. The study was based on the determination of mortality, morphology and walking stability of the insect outside the grain mass, on a flat and smooth surface. Mortality of adults of this Coleoptera in conditions with and without food was similar, which explains the difficulty that this insect had for accessing the food source on the flat and smooth surface. The measurements of body length (BOL), width (BOW) and height (BOH) of R. dominica were compared with those of Tribolium castaneum (Coleoptera: Tenebrionidae), which showed good ability to walk in these conditions. This study indicated that the former presents lower BOL and BOW, and greater BOH than the second, and all these variables showed differences when analyzed simultaneously by means of the construction of multivariate morphometric indices (Width × Height, Length × Height and Height × Length × Width). These morphometric variables, together with the definition of the geometry most similar to the body shape, resulted in determination of the center of gravity (CG) and static rollover threshold (SRTgeom) for both species. Rhyzopertha dominica and T. castaneum presented CGs considered high and low, respectively, and together with the values obtained for SRTgeom, may justify that R. dominica can be considered a less stable species during movement, and presents greater risk of rollover on flat and smooth surfaces. PMID:27097085

  15. Larval RNA interference in the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Linz, David M; Clark-Hachtel, Courtney M; Borràs-Castells, Ferran; Tomoyasu, Yoshinori

    2014-01-01

    The red flour beetle, Tribolium castaneum, offers a repertoire of experimental tools for genetic and developmental studies, including a fully annotated genome sequence, transposon-based transgenesis, and effective RNA interference (RNAi). Among these advantages, RNAi-based gene knockdown techniques are at the core of Tribolium research. T. castaneum show a robust systemic RNAi response, making it possible to perform RNAi at any life stage by simply injecting double-stranded RNA (dsRNA) into the beetle's body cavity. In this report, we provide an overview of our larval RNAi technique in T. castaneum. The protocol includes (i) isolation of the proper stage of T. castaneum larvae for injection, (ii) preparation for the injection setting, and (iii) dsRNA injection. Larval RNAi is a simple, but powerful technique that provides us with quick access to loss-of-function phenotypes, including multiple gene knockdown phenotypes as well as a series of hypomorphic phenotypes. Since virtually all T. castaneum tissues are susceptible to extracellular dsRNA, the larval RNAi technique allows researchers to study a wide variety of tissues in diverse contexts, including the genetic basis of organismal responses to the outside environment. In addition, the simplicity of this technique stimulates more student involvement in research, making T. castaneum an ideal genetic system for use in a classroom setting. PMID:25350485

  16. Larval RNA interference in the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Linz, David M; Clark-Hachtel, Courtney M; Borràs-Castells, Ferran; Tomoyasu, Yoshinori

    2014-10-13

    The red flour beetle, Tribolium castaneum, offers a repertoire of experimental tools for genetic and developmental studies, including a fully annotated genome sequence, transposon-based transgenesis, and effective RNA interference (RNAi). Among these advantages, RNAi-based gene knockdown techniques are at the core of Tribolium research. T. castaneum show a robust systemic RNAi response, making it possible to perform RNAi at any life stage by simply injecting double-stranded RNA (dsRNA) into the beetle's body cavity. In this report, we provide an overview of our larval RNAi technique in T. castaneum. The protocol includes (i) isolation of the proper stage of T. castaneum larvae for injection, (ii) preparation for the injection setting, and (iii) dsRNA injection. Larval RNAi is a simple, but powerful technique that provides us with quick access to loss-of-function phenotypes, including multiple gene knockdown phenotypes as well as a series of hypomorphic phenotypes. Since virtually all T. castaneum tissues are susceptible to extracellular dsRNA, the larval RNAi technique allows researchers to study a wide variety of tissues in diverse contexts, including the genetic basis of organismal responses to the outside environment. In addition, the simplicity of this technique stimulates more student involvement in research, making T. castaneum an ideal genetic system for use in a classroom setting.

  17. Efeitos dentoesqueléticos produzidos pelo aparelho de Herbst na dentadura mista Dentoskeletal treatment effects produced by the Herbst appliance in the mixed dentition

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    Marcio Rodrigues de Almeida

    2006-10-01

    Full Text Available OBJETIVO: o objetivo desta pesquisa clínica prospectiva foi avaliar as alterações cefalométricas dentárias e esqueléticas produzidas pelo aparelho de Herbst em jovens com má oclusão de Classe II, 1ª divisão durante a dentadura mista. METODOLOGIA: trinta jovens (15 do gênero masculino e 15 do feminino com idade média inicial de 9 anos e 10 meses foram tratados com o aparelho de Herbst por um período de 12 meses. Para a comparação dos grupos utilizou-se uma amostra controle de 30 jovens (15 do gênero masculino e 15 do feminino Classe II, 1ª divisão, com idade média inicial de 9 anos e 8 meses, que foram mantidos sem tratamento durante 12 meses. Para cada jovem foram utilizadas duas telerradiografias em norma lateral, obtidas ao início e no final do período de acompanhamento. Utilizou-se um método convencional de avaliação cefalométrica e o método proposto por Pancherz. RESULTADOS E CONCLUSÕES: os resultados deste estudo demonstraram que os efeitos do aparelho de Herbst produzidos na dentadura mista foram primariamente de natureza dentoalveolar. Os incisivos inferiores foram inclinados para vestibular e os superiores foram retruídos; também houve uma extrusão significante dos molares inferiores, enquanto os superiores sofreram restrição de desenvolvimento no sentido vertical. Não houve diferença significante de restrição do crescimento anterior da maxila entre os dois grupos. No sentido vertical da face, a altura facial ântero-inferior se comportou de forma similar, não demonstrando alteração significante entre os grupos. O tratamento com o aparelho de Herbst produziu um aumento modesto, porém, significante no comprimento da mandíbula comparado ao grupo controle. Este aumento, entretanto, foi de menor magnitude que aquele observado em pacientes adolescentes utilizando o mesmo protocolo de tratamento. A correção do overjet (Herbst ocorreu devido a 22% de alterações esqueléticas e 78% de altera

  18. Orthodontic treatment of nongrowing patient with class II division 2 malocclusion by Herbst appliance

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    Nedeljković Nenad

    2009-01-01

    Full Text Available Background. Inheritance is most casual etiological factor of Class II division 2 malocclusion. This kind of malocclusion is very difficult for treatment specially in older patients. Case report. In the female patient, 20 years old, at the beginning of the treatment at the School of Dentistry in Belgrade, lateral cephalogram showed skeletal and dentoalveolar Class II division 2 malocclusion. She was in the Herbst treatment for 8 months and 12 months more with a fixed multibracket appliance. The measurements were performed on lateral cephalograms before and after the treatment: ii, is, mi, ms, Pg and ss. The distance from these points to occlusal perpendicular line (Olp were measured and compared from cephalogram before to cephalogram after the treatment. Temporomandibular joint (TMJ tomograms were compared from before and after the treatment by superimposition. Correction was found in molar and incisor relation, overjet and overbite. There were found sagital skeletal changes and soft tissue profile improvement. Conclusion. Herbst appliance is effective in the treatment of Class II malocclusions, even in adult patients. Dental and skeletal changes as a result of Herbst treatment could be good choice instead of camouflage orthodontics or surgical decision.

  19. The headgear effect of the Herbst appliance: a cephalometric long-term study.

    Science.gov (United States)

    Pancherz, H; Anehus-Pancherz, M

    1993-06-01

    The short- and long-term effects of the Herbst appliance on the maxillary complex were evaluated in 45 patients who had Class II malocclusions. The patients were followed for 6.4 years (5 to 10 years) after treatment. Lateral cephalograms from before and after 7 months of treatment, 6 months, and 6.4 years after treatment were analyzed. During Herbst treatment the upper molars were distalized in 96% of the subjects (maximum 4.5 mm), and the upper molars were intruded in 69% of the subjects (maximum 3.5 mm). The maxillary occlusal plane was opened in 82% of the subjects (maximum 7.5 degrees). Sagittal maxillary jaw base position seemed unaffected by therapy. A downward tipping of the palatal plane was noted in 47% of the subjects (maximum 2 degrees). During the first 6 months after therapy most of the treatment changes reverted. During the following 5.9 years after treatment, normal growth developmental changes prevailed: the upper molars moved mesially, and the teeth extruded, the occlusal plane closed, the maxilla grew anteriorly, and the palatal plane tipped downward. In conclusion the Herbst appliance exhibited a pronounced high-pull headgear effect on the maxillary complex. Without proper retention, however, the effect seemed to be of a temporary nature.

  20. New perspective on Herbst therapy for skeletal Class II malocclusions: a proposal for maxillary protrusion management.

    Science.gov (United States)

    Filho, Leopoldino Capelozza; Siqueira, Danilo Furquim; de Castro, Renata Cristina Faria Ribeiro; An, Tien-Li; Cardoso, Mauricio de Almeida

    2012-01-01

    Angle Class II malocclusions may present morphologic deviations originated from the maxilla, mandible, or both. Since its reintroduction by Pancherz, the Herbst appliance has demonstrated effectiveness in the management of patients with mandibular deficiency. Because of the intermaxillary anchorage, the action of mandibular advancement provokes simultaneous reaction of maxillary restriction, similar to high-pull headgear. This aimed of this report is to compare two cases treated in two phases. In the first interceptive phase, the transverse problem was corrected by rapid maxillary expansion, which was followed by a Herbst appliance for mandibular advancement; in the second corrective phase, the cases were finished with fixed appliances. Although Herbst appliances were used in both patients, one patient with maxillary protrusion and another with mandibular deficiency, their use targeted different types of skeletal discrepancies. This difference allowed for the comparison of treatment effects, and although both patients had their malocclusion corrected, it seems reasonable to conclude that the final outcome was more favorable for the patient with maxillary protrusion.

  1. Clinical effects of fixed functional Herbst appliance in the treatment of class II/1 malocclusion

    Directory of Open Access Journals (Sweden)

    Nedeljković Nenad

    2009-01-01

    Full Text Available Introduction. Sagittal mandible deficiency is the most common cause of skeletal Class II malocclusion. Treatment objective is to stimulate sagittal mandible growth. Fixed functional Herbst appliance use is beneficial for shortening the time required for treatment and does not depend on patient compliance. Case outline. A 13-year-old girl was referred to the Clinic of Orthodontics, School of Dentistry in Belgrade following previous unsuccessful treatment of her skeletal Class II malocclusion using an activator. The patient's poor cooperation had led to failure of the treatment. Patient was subjected to the Herbst treatment for 6 months followed by fixed appliance for another 8 months. Lateral cephalograms before and after the treatment was performed. The remodelation of condylar and fossal articulation was assessed by superimposition of pre- and post-treatment temporomandibular joint tomograms. The promotion of oral hygiene and fluoride use was performed because orthodontic treatment carries a high caries risk and risk for periodontal disease. Skeletal and dental changes were observed after treatment (correction [Max+Mand]: molar relation 7 mm, overjet 8 mm, skeletal relation 5 mm, molars 2 mm, incisors 3 mm. Combination of Herbst and fixed appliances was effective in the treatment of dental and skeletal irregularities for a short period of time. Conclusion . In the retention period, 14 months after treatment, occlusal stability exists. Follow-up care in oral prevention is based on regular recalls at the dental office and supervision at home by the parents.

  2. Control of Sitophilus linearis (Herbst) (Coleoptera: Curculionidae) in Tamarindus indica through of gamma radiation of Cobalt-60

    International Nuclear Information System (INIS)

    The objective of this research was to determine the lethal and sterilization dose of gamma radiation of the Cobalt-60 to control adults of Sitophilus linearis in tamarind fruits. For the accomplishment of the experiment fruits of tamarind were disinfested by gamma radiation with a dose of 1,0 kGy. Each treatment consisted of 4 repetition and each one with 10 fruits, in a total of 40 fruits and 40 insects for treatment. The doses of gamma radiation used were: 0 (test), 50, 100, 150 and 200 Gy, with a dose rate of 1,756 kGy/hora. The experiment was kept in a climatic room with temperature of 25+/-3 deg C and relative humidity of 70+/-5%. The evaluation of the mortality of the insects was made every 7 days after the irradiation. The obtained results showed that the dose of 50 Gy was enough to cause the sterilization and lethal dose was 200 Gy to adult insects of this species

  3. Changes in soft tissue profile following the treatment using a Herbst appliance: A photographic analysis

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    Nedeljković Nenad

    2014-01-01

    Full Text Available Background/Aim. Aesthetics is the reason for most of the class II malocclusion patients to opt for orthodontic treatment. In order to regulate retrognathic profile Herbst appliance for anterior movement of the mandible can be a treatment of choice. The aim of this study was to determine the soft tissue profile changes following Herbst appliance therapy on before and after treatment photos, using the computer program. Methods. This investigation was performed on profile photographs of 20 class II patients (12 females and 8 males aged 18-23 years. Analysis of the changes in soft tissue facial structure relationships evident on the photographs before and after the Herbst appliance therapy was performed using Bentley Micro Station program. The first contour of the soft tissue profile was marked. The following reference lines were subsequently traced: Ricketts aesthetic E line and Juanita line. The area enclosed by these two lines included the nose, upper and lower lip, chin and free space in front of the lips. Using the computer program, the surfaces of the soft tissue structures and free space defined by the reference lines and profile contours were measured. Calculation of the relative proportion of surfaces was done for each photograph. The data obtained were then compared for each patient before and after the treatment. Skeletal and dentoalveolar treatment effects that support soft tissue changes were presented by the profile cephalometric parameters of sagital occlusion (SO analysis. Results. A reduction in the relative surface of the upper lip in males (p < 0.01 and females (p < 0.05 was shown by the pictures. The space occupied by the chin was reduced after the treatment for females only (p < 0.05. The relative surface of the nasal soft tissues, that was included in the reference space was increased (p < 0.01 in both genders. The relationship between the soft tissue and empty surface was changed in favor of the empty surface (p < 0.05 in

  4. TrOn: an anatomical ontology for the beetle Tribolium castaneum.

    Science.gov (United States)

    Dönitz, Jürgen; Grossmann, Daniela; Schild, Inga; Schmitt-Engel, Christian; Bradler, Sven; Prpic, Nikola-Michael; Bucher, Gregor

    2013-01-01

    In a morphological ontology the expert's knowledge is represented in terms, which describe morphological structures and how these structures relate to each other. With the assistance of ontologies this expert knowledge is made processable by machines, through a formal and standardized representation of terms and their relations to each other. The red flour beetle Tribolium castaneum, a representative of the most species rich animal taxon on earth (the Coleoptera), is an emerging model organism for development, evolution, physiology, and pest control. In order to foster Tribolium research, we have initiated the Tribolium Ontology (TrOn), which describes the morphology of the red flour beetle. The content of this ontology comprises so far most external morphological structures as well as some internal ones. All modeled structures are consistently annotated for the developmental stages larva, pupa and adult. In TrOn all terms are grouped into three categories: Generic terms represent morphological structures, which are independent of a developmental stage. In contrast, downstream of such terms are concrete terms which stand for a dissectible structure of a beetle at a specific life stage. Finally, there are mixed terms describing structures that are only found at one developmental stage. These terms combine the characteristics of generic and concrete terms with features of both. These annotation principles take into account the changing morphology of the beetle during development and provide generic terms to be used in applications or for cross linking with other ontologies and data resources. We use the ontology for implementing an intuitive search function at the electronic iBeetle-Base, which stores morphological defects found in a genome wide RNA interference (RNAi) screen. The ontology is available for download at http://ibeetle-base.uni-goettingen.de.

  5. EFFECTS OF ELECTRON BEAM IRRADIATION ON ACTIVITIES OF PEROXIDASE CATALASE AND SUPEROXIDE DISMUTASE OF TRIBOLIUM CASTANEURN (HERBST)%电子束辐照对赤拟谷盗保护酶系的影响

    Institute of Scientific and Technical Information of China (English)

    王殿轩; 王晶磊; 李淑荣; 高美须; 徐威; 林敏; 崔莹

    2011-01-01

    By electron beam irradiation, enzyme activity changes of peroxidase (PCD), catalase (CAT), superoxide dis-mutase (SOD) of Triboliian castaneurn (Herbst) in different insects states (3~4th instar larvae, pupae age 1 day to 2 days and adult for 1 day eclosion) have been investigated. The results showed that POD activity of Tribolium castaneum in different states was in order: adult > pupa> larvae; CAT activity: adult > pupa> larvae; SOD activity: larvae > pupa > adult. After irradiation, the protection enzyme activity rose first, then fell with radiation dose increasing. PCX!) of Tribolivan castaneum in three states reached the highest vitality at 500 Gy, CAT of Tribolium castaneum 3-4th instar larvae, pupae age 1 day to 2 days and adult for 1 day eclosion reached the maximum at 420 Gy and 300Gy respectively, SCO of larvae and pupa up to the maximum at 420 Gy, and adult to the highest at 500 Gy. This test would contribute to illuminate the physiological and biochemical effect of electron beam irradiation on stored grain insects.%研究了赤拟谷盗Tribolium castaneurn( Herbst)不同虫态(3~4龄幼虫、1~2龄蛹和羽化1天成虫)体内过氧化物酶( POD)、过氧化氢酶(CAT)、超氧歧化酶(SOD)活力在电子束辐照条件下的变化.结果表明,赤拟谷盗不同虫态的POD活力:羽化1d成虫>1~2龄蛹>3~4龄幼虫;CAT活力:羽化1d成虫>1~2龄蛹>3~4龄幼虫;SOD活力:3~4龄幼虫>1~2龄蛹>羽化1d成虫.辐照处理后,保护酶活力随辐照剂量的增加呈先上升后下降的趋势,赤拟谷盗3种虫态POD活力均在500 Gy辐照剂量最高;3~4龄幼虫和1~2龄蛹CAT活力在420 Gy辐照剂量最高,羽化1d成虫300 Gy最高;3~4龄幼虫和1~2龄蛹SOD活力在420 Gy辐照剂量最高,羽化1d成虫500 Gy最高.本试验结果有助于阐明电子束辐照对储粮害虫的生理生化效应.

  6. Efficacy of chlorfenapyr against adult Tribofium castaneum exposed on concrete: effects of exposure interval, concentration and the presence of a food source after exposure

    Institute of Scientific and Technical Information of China (English)

    Frank H. Arthur

    2009-01-01

    Chlorfenapyr, an insecticidal pyrrole, was applied to concrete arenas at concentrations of 1.1, 0.825, 0.55, and 0.275 g of active ingredient [AI]/m2. Adult Tribolium castaneum (Herbst), the red flour beetle, were exposed for 2, 4, or 8 h at each concentration, then removed and held either with or without food (wheat flour) for 7 days. Survival was assessed when the beetles were removed from the exposure arenas and daily during the post-exposure period. In the presence of food, survival was high regardless of concentration and the day on which post-treatment survival was assessed, but survival did decrease as the exposure period increased from 4 to 8 h. When the beetles were not given food after exposure, survival at each concentration and exposure period declined during the 1-week post-exposure assessments. This pattern of decrease could be described by linear and non-linear equations. Results show the presence of food material greatly compromised effectiveness of the insecticide, and emphasize the importance of cleaning and sanitation in conjunction with insecticide treatments.

  7. Adult Diapause in Coleoptera

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    Ivo Hodek

    2012-01-01

    Full Text Available Recent studies dealing with adult (reproductive diapause in the Coleoptera are reviewed, as a kind of supplement to the classic compendia. In the first two sections, the general characteristics of adult diapause are described and principal terms explained. Original articles dealing with 19 species from nine coleopteran families (Coccinellidae, Chrysomelidae, Bruchidae, Curculionidae, Carabidae, Silphidae, Scolytidae, Scarabaeidae, and Endomychidae are reviewed. Finally attempts are made at generalisations from the papers reviewed, and hypotheses on diapause evolution are inferred. A polyphenic character of diapause is a prominent feature in C. septempunctata and L. decemlineata, but has been found also in other Coleoptera and in insects generally and often generates voltinism heterogeneity within populations.

  8. UVB Radiation Delays Tribolium castaneum Metamorphosis by Influencing Ecdysteroid Metabolism.

    Science.gov (United States)

    Sang, Wen; Yu, Lin; He, Li; Ma, Wei-Hua; Zhu, Zhi-Hui; Zhu, Fen; Wang, Xiao-Ping; Lei, Chao-Liang

    2016-01-01

    Ultraviolet B (UVB) radiation is an important environmental factor. It is generally known that UVB exhibits high genotoxicity due to causing DNA damage, potentially leading to skin carcinogenesis and aging in mammals. However, little is known about the effects of UVB on the development and metamorphosis of insects, which are the most abundant terrestrial animals. In the present study, we performed dose-response analyses of the effects UVB irradiation on Tribolium castaneum metamorphosis, assessed the function of the T. castaneum prothoracicotropic hormone gene (Trcptth), and analyzed ecdysteroid pathway gene expression profile and ecdysterone titers post-UVB irradiation. The results showed that UVB not only caused death of T. castaneum larvae, but also delayed larval-pupal metamorphosis and reduced the size and emergence rate of pupae. In addition, we verified the function of Trcptth, which is responsible for regulating metamorphosis. It was also found that the expression profiles of Trcptth as well as ecdysteroidogenesis and response genes were influenced by UVB radiation. Therefore, a disturbance pulse of ecdysteroid may be involved in delaying development under exposure to irradiation. To our knowledge, this is the first report indicating that UVB can influence the metamorphosis of insects. This study will contribute to a better understanding of the impact of UVB on signaling mechanisms in insect metamorphosis.

  9. UVB Radiation Delays Tribolium castaneum Metamorphosis by Influencing Ecdysteroid Metabolism.

    Directory of Open Access Journals (Sweden)

    Wen Sang

    Full Text Available Ultraviolet B (UVB radiation is an important environmental factor. It is generally known that UVB exhibits high genotoxicity due to causing DNA damage, potentially leading to skin carcinogenesis and aging in mammals. However, little is known about the effects of UVB on the development and metamorphosis of insects, which are the most abundant terrestrial animals. In the present study, we performed dose-response analyses of the effects UVB irradiation on Tribolium castaneum metamorphosis, assessed the function of the T. castaneum prothoracicotropic hormone gene (Trcptth, and analyzed ecdysteroid pathway gene expression profile and ecdysterone titers post-UVB irradiation. The results showed that UVB not only caused death of T. castaneum larvae, but also delayed larval-pupal metamorphosis and reduced the size and emergence rate of pupae. In addition, we verified the function of Trcptth, which is responsible for regulating metamorphosis. It was also found that the expression profiles of Trcptth as well as ecdysteroidogenesis and response genes were influenced by UVB radiation. Therefore, a disturbance pulse of ecdysteroid may be involved in delaying development under exposure to irradiation. To our knowledge, this is the first report indicating that UVB can influence the metamorphosis of insects. This study will contribute to a better understanding of the impact of UVB on signaling mechanisms in insect metamorphosis.

  10. Gamma radiation effects on the reproductive capacity of Tribolium castaneum

    Energy Technology Data Exchange (ETDEWEB)

    Hu, T.; Tsai, L.T.; Fu, Y.K.

    1984-10-01

    Unmated pairs of Tribolium castaneum (Hbst.) were treated with gamma radiation from 8.2 to 65.6 Gy at 8.2-Gy increment and allowed to mate and oviposit. The number of larva per fertile pair and the percentage of sterility at each dosage were determined. The regression line for logarithm of average number of larva progeny per pair and a probit sterility curve were calculated. The average number of progeny per pair was reduced 10% by a dosage of 28.99 Gy. The probit sterility line indicated that 107 Gy in the sterilizing dosage for this species. 9 refs., 2 figs., 1 tab.

  11. Primeira ocorrência de Polybius navigator (Herbst) (Decapoda, Brachyura, Portunidae) no Atlântico ocidental First occurrence of Polybius navigator (Herbst) (Decapoda, Brachyura, Portunidae) in Western Atlantic

    OpenAIRE

    Gustavo Augusto Schmidt de Melo; Thais Brandini Crivelaro

    2002-01-01

    Among the portunid crabs collected during the Ilha Grande Project (1966-1969), one specimen of Polybius navigator (Herbst, 1794) was found. This species formerly known from the Eastern Atlantic, Mediterranean and Adriatic and Black Seas is redescribed, illustrated and comments on the new status of the genus is done. It is the first record of this species for the Brazilian coast and Western Atlantic.

  12. Primeira ocorrência de Polybius navigator (Herbst (Decapoda, Brachyura, Portunidae no Atlântico ocidental First occurrence of Polybius navigator (Herbst (Decapoda, Brachyura, Portunidae in Western Atlantic

    Directory of Open Access Journals (Sweden)

    Gustavo Augusto Schmidt de Melo

    2002-03-01

    Full Text Available Among the portunid crabs collected during the Ilha Grande Project (1966-1969, one specimen of Polybius navigator (Herbst, 1794 was found. This species formerly known from the Eastern Atlantic, Mediterranean and Adriatic and Black Seas is redescribed, illustrated and comments on the new status of the genus is done. It is the first record of this species for the Brazilian coast and Western Atlantic.

  13. Odoriferous Defensive stink gland transcriptome to identify novel genes necessary for quinone synthesis in the red flour beetle, Tribolium castaneum.

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    Jianwei Li

    Full Text Available Chemical defense is one of the most important traits, which endow insects the ability to conquer a most diverse set of ecological environments. Chemical secretions are used for defense against anything from vertebrate or invertebrate predators to prokaryotic or eukaryotic parasites or food competitors. Tenebrionid beetles are especially prolific in this category, producing several varieties of substituted benzoquinone compounds. In order to get a better understanding of the genetic and molecular basis of defensive secretions, we performed RNA sequencing in a newly emerging insect model, the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae. To detect genes that are highly and specifically expressed in the odoriferous gland tissues that secret defensive chemical compounds, we compared them to a control tissue, the anterior abdomen. 511 genes were identified in different subtraction groups. Of these, 77 genes were functionally analyzed by RNA interference (RNAi to recognize induced gland alterations morphologically or changes in gland volatiles by gas chromatography-mass spectrometry. 29 genes (38% presented strong visible phenotypes, while 67 genes (87% showed alterations of at least one gland content. Three of these genes showing quinone-less (ql phenotypes - Tcas-ql VTGl; Tcas-ql ARSB; Tcas-ql MRP - were isolated, molecularly characterized, their expression identified in both types of the secretory glandular cells, and their function determined by quantification of all main components after RNAi. In addition, microbe inhibition assays revealed that a quinone-free status is unable to impede bacterial or fungal growth. Phylogenetic analyses of these three genes indicate that they have evolved independently and specifically for chemical defense in beetles.

  14. LETHAL EFFECT OF DIFFERENT TREATMENT TEMPERATURES ON TRIBOLIUM CA STA NEUM ( HERBST )%不同温度处理对赤拟谷盗的致死作用

    Institute of Scientific and Technical Information of China (English)

    吕建华; 钟建军; 王洁静; 史雅; 翟卫波

    2012-01-01

    采用水浴加热,研究不同温度(35℃、45℃、55℃、60℃、65℃)下处理不同时间(60 s、75 s、90 s、105 s、120 s)后对小麦粉中的赤拟谷盗幼虫、蛹和成虫的致死作用.研究结果表明,不同处理温度、不同处理时间对赤拟谷盗幼虫、蛹和成虫的致死作用效果差异显著.在处理温度为35℃、45℃时,各个处理时间对赤拟谷盗幼虫、蛹和成虫正常发育没有影响,而在处理温度为55℃以上时对赤拟谷盗幼虫、蛹和成虫具有较强的致死作用.在处理温度为65℃时,处理60 s以上对赤拟谷盗成虫的致死作用效果即达到100%,处理75 s可100%抑制赤拟谷盗蛹正常发育为成虫,处理90 s以上能完全抑制赤拟谷盗幼虫正常发育为成虫.%In this paper, we studied the lethal effect of different treatment temperatures (35 ℃,45 ℃,55 ℃,60 ℃ and 65 ℃)on the adults, pupae and larvae of Tribolium castaneum (Herbst) in wheat flour by water bath heating for different times (60 s,75 s,90 s, 105 s and 120 s). The results showed that the lethal effects of the different treatment temperatures and different treatment times on the adults,pupae and larvae of T. castaneum had significant difference. At 35 ℃ and 45 ℃,treatment time had no influence on the normal development of the adults,pupae and larvae of T. castaneum. The temperature higher than 55 ℃ had strong lethal effect on the adults,pupae and larvae of T. custaneum. For the treatment at 65 ℃,the mortality of the adults reached 100% after treatment for more than 60 seconds, 100% pupae could be suppressed from developing into adults after treatment for 75 seconds,and the larvae could be completely suppressed from developing into adults after treatment for 90 seconds.

  15. Effect of phosphine on the activities of CAT and SOD in Tribolium castaneum%磷化氢对赤拟谷盗成虫体内CAT和SOD活性的影响

    Institute of Scientific and Technical Information of China (English)

    魏朝明; 张琳琳; 廉振民

    2007-01-01

    用赤拟谷盗Tribolium castaneum(Herbst)成虫,研究PH3对其体内超氧化物岐化酶(SOD)和过氧化氢酶(CAT)活性的影响.结果表明PH3熏蒸后,4种不同地理种群的成虫体内,CAT酶活性都有所降低.种群NGD1降低最多,接近57%,而NGD4则只降低了19%,NGD2和NGD3的降低都在40%左右.不同地理种群的成虫体内,SOD酶活性在熏蒸后都有所增加,种群NGD1增加幅度最大,NGD2增加幅度最小.SOD和CAT活性的变化幅度与成虫对PH3产生的抗性有关,抗性越大,酶活性变化越大.

  16. STUDIES ON THE FUMIGATION ACTIVITY OF FOUR PRICKLY ASH EXTRACTS TO THE ADULTS OF TRIBOLIUM CASTANEUM%四种花椒提取物对赤拟谷盗成虫熏蒸活性的研究

    Institute of Scientific and Technical Information of China (English)

    聂霄艳; 邓永学; 王进军; 刘映红; 李俊

    2007-01-01

    在30℃下采用广口瓶密闭熏蒸法熏蒸48 h和72 h,测定青椒精油、红椒精油、一级麻素及二级麻素对赤拟谷盗(Tribolium castaneum Herbst)成虫的熏蒸致死作用及LC50值.结果表明LC50值大小顺序为一级麻素>二级麻素>红椒精油>青椒精油.进一步研究熏蒸时间和温度对青椒精油及红椒精油熏蒸活性的影响,结果表明熏蒸时间越长,LC50值越小,两种花椒精油的持效期较长;随着温度的升高,同一精油浓度下赤拟谷盗的校正死亡率呈现先增大后减小的趋势.

  17. Toxicity comparison of ethyl formate on different resistant strains of Tribolium castaneum to phosphine%甲酸乙酯对不同磷化氢抗性水平赤拟谷盗的毒力比较

    Institute of Scientific and Technical Information of China (English)

    王殿轩; 刘炎; 郜智贤; 徐东; 储昭兵

    2010-01-01

    参考FAO推荐的熏蒸剂毒力测定方法,测定了甲酸乙酯对4种不同磷化氢抗性水平的赤拟谷盗(Tribolium castaneum(Herbst))品系的毒力.主要结果为甲酸乙酯对4个磷化氢抗性系数分别为1、3、170和247倍的赤拟谷盗品系的LD50值相应地为17.354L/m3、18.776L/m3、20.464L/m3和21.470 mL/m3.结果表明,不同磷化氢抗性的赤拟谷盗品系对甲酸乙酯的敏感相似,对磷化氢具有明显抗性的赤拟谷盗对甲酸乙酯未表现出抗性.

  18. The natural diet of the mud crab Scylla olivacea (Herbst, 1896) in Pichavaram mangroves, India.

    Science.gov (United States)

    Viswanathan, C; Raffi, S M

    2015-11-01

    Food and feeding habits of mud crab Scylla olivacea (Herbst, 1896) in Pichavaram mangroves was investigated quantitatively and qualitatively for a period of two years from June 2010 to May 2012. Gut contents from 1737 specimens comprising 843 males and 894 females in the size range between 45 mm and 148 mm were examined. Crustaceans form the predominant food item in a majority of size groups in terms of percentage wet weight and frequency of occurrence, while molluscs showed a preference in few size groups. The other dietary items includes fishes, detritus, mud and sand and miscellaneous. Gut content analysis revealed no significant variation between the quantities of food consumed by both sexes. Feeding intensity was higher in juveniles and subadults of both sexes than that of adults, revealing a greater preference to feed on fast moving prey such as crustaceans and fishes. The results of the present study indicate that S. olivacea in Pichavaram mangroves exhibited a clear preference for crustaceans.

  19. The natural diet of the mud crab Scylla olivacea (Herbst, 1896) in Pichavaram mangroves, India.

    Science.gov (United States)

    Viswanathan, C; Raffi, S M

    2015-11-01

    Food and feeding habits of mud crab Scylla olivacea (Herbst, 1896) in Pichavaram mangroves was investigated quantitatively and qualitatively for a period of two years from June 2010 to May 2012. Gut contents from 1737 specimens comprising 843 males and 894 females in the size range between 45 mm and 148 mm were examined. Crustaceans form the predominant food item in a majority of size groups in terms of percentage wet weight and frequency of occurrence, while molluscs showed a preference in few size groups. The other dietary items includes fishes, detritus, mud and sand and miscellaneous. Gut content analysis revealed no significant variation between the quantities of food consumed by both sexes. Feeding intensity was higher in juveniles and subadults of both sexes than that of adults, revealing a greater preference to feed on fast moving prey such as crustaceans and fishes. The results of the present study indicate that S. olivacea in Pichavaram mangroves exhibited a clear preference for crustaceans. PMID:26586996

  20. Curative activity of insecticides against plum curculio (Coleoptera: Curculionidae) in tart cherries.

    Science.gov (United States)

    Hoffmann, Eric J; Vandervoort, Christine; Wise, John C

    2009-10-01

    Tart cherry, Prunus cerasus L. variety Montmorency, fruit were infested with plum curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), and treated with insecticides to target late instars, neonates, and eggs. The organophosphates azinphos-methyl and phosmet and the neonicotinoid thiamethoxam reduced larval emergence rates by >90% for all life stage targets; after >30 d, few surviving larvae were found inside fruit. Acetamiprid and thiacloprid also had curative activity and yielded >75% reductions in emergence and few surviving larvae in the fruit after >30 d. The juvenile hormone analog pyriproxyfen reduced larval emergence, but 66% of fruit that was treated to target late-instars still had live larvae inside of them after >30 d. Novaluron, chlorantraniliprole, and esfenvalerate had no curative activity. Indoxacarb had limited curative activity, and all targeted life stages had larval emergence. Internal and external residues were analyzed and are discussed in relation to their penetration and curative potential. The curative activity of azinphos-methyl has played an important role in meeting federal standards for infestation-free tart cherries at processing. Regulatory changes are eliminating the use of this compound, and new integrated pest management programs for plum curculio will need to address the loss of azinphos-methyl's curative activity. PMID:19886451

  1. Tribolium castaneum larval gut transcriptome and proteome: A resource for the study of the coleopteran gut.

    Science.gov (United States)

    Morris, Kaley; Lorenzen, Marcé D; Hiromasa, Yasuaki; Tomich, John M; Oppert, Cris; Elpidina, Elena N; Vinokurov, Konstantin; Jurat-Fuentes, Juan Luis; Fabrick, Jeff; Oppert, Brenda

    2009-08-01

    Tribolium castaneum is an important agricultural pest and an advanced genetic model for coleopteran insects. We have taken advantage of the recently acquired T. castaneum genome to identify T. castaneum genes and proteins in one of the more critical environmental interfaces of the insect, the larval alimentary tract. Genetic transcripts isolated from the T. castaneum larval gut were labeled and hybridized to a custom array containing oligonucleotides from predicted genes in the T. castaneum genome. Through a ranking procedure based on relative labeling intensity, we found that approximately 17.6% of the genes represented in the array were predicted to be highly expressed in gut tissue. Several genes were selected to compare relative expression levels in larval gut, head, or carcass tissues using quantitative real-time PCR, and expression levels were, with few exceptions, consistent with the gut rankings. In parallel with the microarrays, proteins extracted from the T. castaneum larval gut were subjected to proteomic analysis. Two-dimensional electrophoretic analysis combined with MALDI-TOF resulted in the identification of 37 of 88 selected protein samples. As an alternative strategy, one-dimensional electrophoretic separation of T. castaneum larval gut proteins followed by two-dimensional nano-HPLC and ESI-MS/MS resulted in the identification of 98 proteins. A comparison of the proteomic studies indicated that 16 proteins were commonly identified in both, whereas 80 proteins from the proteomic analyses corresponded to genes with gut rankings indicative of high expression in the microarray analysis. These data serve as a resource of T. castaneum transcripts and proteins in the larval gut and provide the basis for comparative transcriptomic and proteomic studies related to the gut of coleopteran insects.

  2. Identification of maternally-loaded RNA transcripts in unfertilized eggs of Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Preuss Kevin M

    2012-11-01

    Full Text Available Abstract Background Maternal RNAs play a critical role in early development. Variation in the diversity and levels of maternally derived gene transcripts may be central to the origin of phenotypic novelty -- a longstanding problem in evolution and development. By studying maternal transcriptomes within and between divergent species, a better understanding of the evolutionary forces acting on maternal RNA allocation is possible. Results We present the first maternal transcriptome of the red flour beetle, Tribolium castaneum. Using a tiled whole-genome microarray, we found that 58.2% of T. castaneum genes are maternally loaded into eggs. Comparison of known Drosophila melanogaster maternal genes to our results showed widespread conservation of maternal expression with T. castaneum. Additionally, we found that many genes previously reported as having sex or tissue specific expression in T. castaneum were also maternally loaded. Identification of such pleiotropy is vital for proper modeling and testing of evolutionary theory using empirical data. The microarray design also allowed the detection of 2315 and 4060 novel transcriptionally active regions greater in length than 100 bp in unfertilized and fertilized T. castaneum eggs, respectively. These transcriptionally active regions represent novel exons of potentially unknown genes for future study. Conclusions Our results lay a foundation for utilizing T. castaneum as a model for understanding the role of maternal genes in evolution.

  3. 1825 (Coleoptera: Cerambycidae

    Directory of Open Access Journals (Sweden)

    Márcia d´Avila

    2006-01-01

    Full Text Available The erva-mate (Ilex paraguariensis A.St.-Hil. has a social, cultural and economic importance in the southern states of Brazil. The pure stands of this culture was responsible for the increase of many species of insects. Hedypathes betulinus (Klug, 1825 (Coleoptera: Cerambycidae is considered the main pest from an economic viewpoint because of its difficult control and potential for damage. The larval phase occurs inside the twings and trunks, what makes more difficult to deal with its detention and management. Cultural and mechanical management are the most indicated, such as collection of adults, prunning and burning of plant parts damaged by the insect, balanced nutrition, adequate plant density and maintenance of areas with native vegetation or also the introduction of policulture. These strategies may increase the agroecossystem balance and thus a reduction of the insect-pest to an aceptable level. Therefore, the objective of this study was to assemble and the discuss the information on the bioecology and management of erva-mate borer.

  4. Tratamento ortopédico com aparelho de Herbst: ocorrem mudanças verticais no padrão de crescimento facial? Orthopedic treatment with the Herbst appliance: do vertical changes occur in the facial growth pattern?

    Directory of Open Access Journals (Sweden)

    Luís Antônio de Arruda Aidar

    2009-12-01

    Full Text Available OBJETIVO: o objetivo deste estudo prospectivo de 32 adolescentes com má oclusão de Classe II, divisão 1, associada a retrognatismo mandibular, tratados com aparelho de Herbst, construído sobre bandas e coroas metálicas, foi avaliar cefalometricamente as possíveis mudanças no padrão de crescimento facial. METODOLOGIA: as telerradiografias laterais foram obtidas ao início do tratamento (T1 e imediatamente após 12 meses de tratamento com o referido aparelho ortopédico (T2. Foram utilizados o quociente de Jarabak e o VERT de Ricketts (modificado para determinação do padrão facial em T1 e T2. RESULTADOS: utilizando o quociente de Jarabak, os resultados evidenciaram que 27 casos (84,4% apresentaram padrões hipodivergentes em T1 e permaneceram da mesma forma em T2. Cinco casos (15,6% apresentaram padrão neutro em T1 e não exibiram mudanças em T2. Quando avaliado o VERT de Ricketts (modificado, não ocorreram mudanças no padrão facial em 31 pacientes. Em apenas um caso ocorreu mudança do tipo facial. CONCLUSÃO: baseado nos resultados obtidos, pode-se concluir que, após 12 meses de tratamento com aparelho de Herbst, não ocorreram mudanças verticais no padrão de crescimento facial dos pacientes estudados.OBJECTIVE: The aim of this prospective study was to evaluate through the exam of cephalograms the occurrence of changes in facial growth pattern. The sample was composed by 32 adolescent with Angle Class II, division 1 malocclusion and mandibular retrognathism. All patients were treated with the Herbst appliance built over bands and metal crowns. METHODOLOGY: Lateral cephalograms were taken at the beginning of treatment (T1 and immediately after 12 months of treatment with Herbst appliance (T2. Jarabak's coefficient and Ricketts' VERT (modified were used to establish the facial pattern in T1 and T2. RESULTS: According to Jarabak's coefficient, in T1, 84.4% (27 cases presented hypodiverging pattern and 15.6% (5 cases presented

  5. Oenocyte development in the red flour beetle Tribolium castaneum.

    Science.gov (United States)

    Burns, Kevin A; Gutzwiller, Lisa M; Tomoyasu, Yoshinori; Gebelein, Brian

    2012-04-01

    Oenocytes are a specialized cell type required for lipid processing, pheromone secretion, and developmental signaling. Their development has been well characterized in Drosophila melanogaster, but it remains unknown whether the developmental program is conserved in other insect species. In this study, we compare and contrast the specification and development of larval oenocytes between Drosophila and the red flour beetle, Tribolium castaneum. First, we identify several useful reagents to label larval oenocytes, including both a Tribolium GFP enhancer trap line and a simple flurophore-conjugated streptavidin staining method that recognizes oenocytes across insect species. Second, we use these tools to describe oenocyte development in Tribolium embryos, and our findings provide evidence for conserved roles of MAP kinase signaling as well as the Spalt, Engrailed, hepatocyte nuclear factor-4, and ventral veins lacking factors in producing abdominal-specific oenocyte cells. However, Tribolium embryos produce four times as many oenocytes per abdominal segment as Drosophila, and unlike in Drosophila, these cells rapidly downregulate the expression of the Spalt transcription factor. Thus, these results provide new insight into the molecular pathways regulating oenocyte specification across insect species.

  6. Structure of the Tribolium castaneum Telomerase Catalytic Subunit TERT

    Energy Technology Data Exchange (ETDEWEB)

    Gillis,A.; Schuller, A.; Skordalakes, E.

    2008-01-01

    A common hallmark of human cancers is the overexpression of telomerase, a ribonucleoprotein complex that is responsible for maintaining the length and integrity of chromosome ends. Telomere length deregulation and telomerase activation is an early, and perhaps necessary, step in cancer cell evolution. Here we present the high-resolution structure of the Tribolium castaneum catalytic subunit of telomerase, TERT. The protein consists of three highly conserved domains, organized into a ring-like structure that shares common features with retroviral reverse transcriptases, viral RNA polymerases and B-family DNA polymerases. Domain organization places motifs implicated in substrate binding and catalysis in the interior of the ring, which can accommodate seven to eight bases of double-stranded nucleic acid. Modelling of an RNA-DNA heteroduplex in the interior of this ring demonstrates a perfect fit between the protein and the nucleic acid substrate, and positions the 3'-end of the DNA primer at the active site of the enzyme, providing evidence for the formation of an active telomerase elongation complex.

  7. Expression patterns of cysteine peptidase genes across the Tribolium castaneum life cycle provide clues to biological function

    Science.gov (United States)

    The red flour beetle, Tribolium castaneum, is a major agricultural pest responsible for considerable loss of stored grain and cereal products worldwide. T. castaneum larvae have a highly compartmentalized gut, with cysteine peptidases mostly in the acidic anterior part of the midgut. We have descri...

  8. Avaliação da quantidade de movimentação dos molares superiores com emprego do aparelho de Herbst Assessment of the displacement of the upper molars using the Herbst appliance

    Directory of Open Access Journals (Sweden)

    Paulo Cezar Rodrigues Ogeda

    2004-08-01

    Full Text Available O Herbst é um aparelho ortopédico funcional fixo destinado principalmente ao estímulo de crescimento mandibular durante a correção da má oclusão de Classe II esquelética de pacientes em crescimento. Várias publicações descreveram os efeitos deste aparelho durante a correção da Classe II, revelando a promoção de alterações esqueléticas e dentárias em igual proporção, favorecendo sua correção. Parte do movimento dentário ocorre por distalização dos primeiros molares superiores. Esta pesquisa teve como objetivo avaliar a quantidade e o tipo de movimento distal ocorrido com os primeiros molares superiores permanentes, e a conseqüência destes movimentos sobre o plano oclusal funcional durante o período de utilização do aparelho de Herbst. A amostra foi composta de 22 pacientes portadores da má oclusão de classe II esquelética, retrognatismo mandibular, com idade média de 12 anos e 11 meses, tratados por um período médio de 10,1 meses. Os aparelhos foram construídos utilizando a ancoragem total no arco maxilar, visando potencializar a ação ortopédica e minimizar a perda de ancoragem. As alterações foram medidas em cefalogramas específicos obtidos das telerradiografias em norma lateral tomadas em dois tempos: tempo 1 (T1 antes da instalação do aparelho, e tempo 2 (T2, após sua remoção. Medidas cefalométricas lineares e angulares em relação ao plano horizontal de Frankfurt, plano palatino e a uma linha vertical de referência a partir do ponto S perpendicular a Frankfurt, foram utilizadas para quantificar os deslocamentos dos primeiros molares superiores. Foram avaliados: o deslocamento distal médio das coroas, o deslocamento distal médio de suas raízes, a conseqüente inclinação no longo eixo dos molares durante a distalização, o deslocamento vertical em relação ao plano palatino, e finalmente, a conseqüência da variação vertical do primeiro molar sobre o plano oclusal funcional. Os

  9. ANTHONOMUS RUBI (HERBST, 1795 (COLEOPTERA: CURCULIONIDAE A NEW DANGEROUS PEST IN THE ECOLOGICAL CROPS OF STRAWBERRY IN THE SOUTHERN REGIONS OF ROMANIA

    Directory of Open Access Journals (Sweden)

    Traian MANOLE

    2013-12-01

    Full Text Available In Romania mostly of 70% of strawberry ecological plantations were cultivated with specific commercial cultivars like Premial, Red Gauntlet, Dana and Elsanta. In the last years became to be cropped cultivars with increased resistance to various pest insects, specially in the first stages of vegetation period like Honeoye, Elsanta, Camorosa, Marmolada, Chandler, Darselect, Clery and Sonata. On the little individual farms with small surfaces the cultivars Senga Sengana, Marmolada, Elsinore should be the primary source of infestation with Anthonomus rubi.A study of distribution, density and the degree attack of Anthonomus rubi was carried out in the period 2006-2007 in the Vidra and Comana localities, Giurgiu district in the southern part of Romania. The study and observations that was made showed that this species is increased in density in the new ecological plantations of strawberry from some individual farms. The strawberry blossom weevil is not a common pest of strawberry plantations in Romania but some cultivars cropped in ecological system became more sensitive to the weevil attack by increasing in density. Observations and researches were carried out on 4 cultivars and showed that the degree of damage caused by A. rubi depends of cultivar morphological characteristics (small fruits or phenophases (time of ripening and duration of budding and blossoming.

  10. Antifeedant Diterpenoids against Tribolium castaneum from the Stems and Twigs of Ceriops tagal (Rhizophoraceae

    Directory of Open Access Journals (Sweden)

    Zhi Wei Deng

    2011-07-01

    Full Text Available The screening of several Chinese mangrove plants for insecticidal principles showed that ethanol extract of Ceriops tagal stems and twigs possessed significant feeding deterrent activity against the red flour beetle, Tribolium castaneum (Family: Rhizophoraceae. From the ethanol extract, three feeding deterrent diterpenoids were isolated by bioassay-guided fractionation. The compounds were identified as tagalsin A, B, and H on the basis of their phytochemical and spectral data. Tagalsin A, B, and H exhibited strong feeding deterrent activity against T. castaneum adults with EC50 values of 375.3 ppm, 277.3 ppm, and 285.45 ppm, respectively.

  11. Complete sequence and characterization of mitochondrial genome in the swimming crab Portunus sanguinolentus (Herbst, 1783) (Decapoda, Brachyura, Portunidae).

    Science.gov (United States)

    Meng, Xianliang; Jia, Fulong; Zhang, Xiaohui; Liu, Ping; Li, Jian

    2016-07-01

    The three-spot swimming crab Portunus sanguinolentus (Herbst, 1783) is a commercially important fishery species, widely distributed in the Indo-Pacific region. In this study, we present the complete mitochondrial genome of P. sanguinolentus. The genome is 16 027 bp in length with circular organization, encoding the standard set of 13 protein-coding genes, 22 tRNA genes, and two rRNA genes. The overall A + T content is 65.60%, which is lower than that of its congeneric species Portunus pelagicus and Portunus trituberculatus. The mitogenome carries 1254 bp of intergenic region constituting 7.82% of the genome, and six pairs of overlapping genes with the overlap size from 1 to 7 bp. The complete mitogenome sequence information of P. sanguinolentus would provide useful data for further studies on population genetics and molecular systematics. PMID:26153754

  12. Abundance of microsatellites in the entire genome and EST of Tribolium castaneum%赤拟谷盗全基因组和EST中微卫星的丰度

    Institute of Scientific and Technical Information of China (English)

    张琳琳; 魏朝明; 廉振民; 孔光耀

    2008-01-01

    微卫星是近年大力开发的一种分子标记,为了推进赤拟谷盗Tribolium castaneum(Herbst)遗传学相关研究,对赤拟谷盗全基因组和EST中由1~6个碱基重复单元组成的简单序列重复进行分析,进而对其微卫星的丰度和分布进行比较分析.微卫星在赤拟谷盗EST中的分布频率为I/0.87 kb,其中单碱基重复序列占71.25%,是最丰富的重复单元,而六、三、四、二,五碱基重复单元序列分别占23.93%,2.94%,1.56%,0.17%,0.15%.全基因组中微卫星的分布频率为1/3.65 kb,其中六碱基重复序列占61.96%,是最丰富的重复单元,而三,四,一,五,二碱基重复单元序列分别占14.35%,13.75%,4.68%,3.60%,1.69%.同时发现富含A和T碱基的微卫星占主导地位,富含G和C碱基的微卫星数量较少.进一步的分析显示,微卫星在每条染色体上的丰度存在很大的相似性.

  13. Lactic Acid Contents of Tribolium castaneum Insect Stages in 5% and 2% Low Oxygen Atmosphere%2%和5%低氧处理赤拟谷盗不同虫态的乳酸测定研究

    Institute of Scientific and Technical Information of China (English)

    周思旭; 曹阳; 李光涛; 张涛羽

    2009-01-01

    用乳酸测试盒测定了正常大气下5%和2%氧浓度处理24 h的赤拟谷盗(Tribolium castaneum Herbst)卵、幼虫、蛹和成虫的体内乳酸含量.结果表明:正常大气下,4种虫态试虫体内的乳酸含量明显不同,成虫的乳酸含量最高,蛹次之,幼虫较小,卵最低;雌成虫和雄成虫体内的乳酸含量,差异不显著(P>0.05).在5%氧浓度下处理24 h,前述4种虫态的乳酸含量分别是对照组相同虫态乳酸含量的2.1倍、2.5倍、2.3倍和1.1倍.2%氧浓度下处理24 h,4虫态的乳酸含量明显比5%氧浓度处理组各相同虫态的高,分别是对照组相同虫态乳酸含量的4.7倍、3.4倍、4.5倍和1.5倍.低氧条件下,四种虫态的乳酸含量都明显增加,卵和蛹的乳酸含量增加的最快,幼虫次之,成虫最慢.因此,低氧导致害虫各个虫态体内乳酸量的增加与低氧致死机理有一定的关系.

  14. DIRS retroelements in arthropods: identification of the recently active TcDirs1 element in the red flour beetle Tribolium castaneum.

    Science.gov (United States)

    Goodwin, T J D; Poulter, R T M; Lorenzen, M D; Beeman, R W

    2004-08-01

    Members of the DIRS family of retrotransposons differ from most other known retrotransposons in that they encode a tyrosine recombinase (YR), a type of enzyme frequently involved in site-specific recombination. This enzyme is believed to insert the extrachromosomal DNA intermediate of DIRS element retrotransposition into the host genome. DIRS elements have been found in plants, a slime mold, fungi, and a variety of animals including vertebrates, echinoderms and nematodes. They have a somewhat patchy distribution, however, apparently being absent from a number of model organisms such as Saccharomyces cerevisiae, Arabidopsis thaliana and Drosophila melanogaster. In this report we describe the first DIRS retroelement to be identified in an arthropod. This element, TcDirs1, was found in the red flour beetle Tribolium castaneum (Coleoptera). It is generally similar in sequence and structure to several previously described members of the DIRS group: it is bordered by inverted terminal repeats and it has a similar set of protein-coding domains (Gag, reverse transcriptase/ribonuclease H, and the YR), although these are arranged in a novel fashion. TcDirs1 elements exhibit several features indicative of recent activity, such as intact coding regions, a high level of sequence similarity between distinct elements and polymorphic insertion sites. Given their presence in an experimentally tractable host, these potentially active elements might serve as useful models for the study of DIRS element retrotransposition. An element closely related to TcDirs1 was also detected in sequences from a second arthropod, the honey bee Apis mellifera (Hymenoptera), suggesting that these retrotransposons are long-term residents of arthropod genomes. PMID:15221458

  15. Forward genetics in Tribolium castaneum: opening new avenues of research in arthropod biology

    OpenAIRE

    Peel, Andrew D

    2009-01-01

    A recent paper in BMC Biology reports the first large-scale insertional mutagenesis screen in a non-drosophilid insect, the red flour beetle Tribolium castaneum. This screen marks the beginning of a non-biased, 'forward genetics' approach to the study of genetic mechanisms operating in Tribolium. See research article http://biomedcentral.com/1741-7007/7/73

  16. Methuselah-like genes affect development, stress resistance, lifespan and reproduction in Tribolium castaneum.

    Science.gov (United States)

    Li, Chengjun; Zhang, Yi; Yun, Xiaopei; Wang, Yanyun; Sang, Ming; Liu, Xing; Hu, Xingxing; Li, Bin

    2014-10-01

    Methuselah (Mth) is associated with lifespan, stress resistance and reproduction in Drosophila melanogaster, but Mth is not present in nondrosophiline insects. A number of methuselah-likes (mthls) have been identified in nondrosophiline insects, but it is unknown whether the functions of mth are shared by mthls or are divergent from them. Five mthls have been identified in Tribolium castaneum. Although they have different developmental expression patterns, they all enhance resistance to starvation. Only mthl1 and mthl2 enhance resistance to high temperature, whereas mthl4 and mthl5 negatively regulate oxidative stress in T. castaneum. Unlike in the fly with mth mutation, knockdown of mthls, except mthl3, shortens the lifespan of T. castaneum. Moreover, mthl1 and mthl2 are critical for Tribolium development. mthl1 plays important roles in larval and pupal development and adult eclosion, while mthl2 is required for eclosion. Moreover, mthl1 and mthl2 silencing reduces the fertility of T. castaneum, and mthl1 and mthl4 are also essential for embryo development. In conclusion, mthls have a significant effect on insect development, lifespan, stress resistance and reproduction. These results provide experimental evidence for functional divergence among mthls/mth and clues for the signal transduction of Mthls.

  17. Direct and indirect genetic effects in life-history traits of flour beetles (Tribolium castaneum)

    NARCIS (Netherlands)

    Ellen, Esther D.; Peeters, Katrijn; Verhoeven, Merel; Gols, Rieta; Harvey, Jeffrey A.; Wade, Michael J.; Dicke, Marcel; Bijma, Piter

    2016-01-01

    Indirect genetic effects (IGEs) are the basis of social interactions among conspecifics, and can affect genetic variation of nonsocial and social traits. We used flour beetles (Tribolium castaneum) of two phenotypically distinguishable populations to estimate genetic (co)variances and the effect of

  18. Tcmof regulates larval/pupal development and female fecundity in red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Wang, Yanyun; Li, Chengjun; Sang, Ming; Li, Bin

    2015-02-01

    Males absent on the first (MOF) was originally identified as an essential component of the X chromosome dosage compensation system in Drosophila melanogaster, and is also a member of the MYST family of histone acetyltransferases. MOF has been extensively studied in D. melanogaster and mammals. However, whether MOF is involved in dosage compensation and/or other vital functions for newly emerging model insects such as Tribolium castaneum, is unclear. We cloned the mof from T. castaneum, named Tcmof. Phylogenetic analysis revealed that mof is highly conserved in eukaryotes but lost in birds. qPCR showed that Tcmof was most highly expressed in the early embryo stage and equally expressed in males and females. Treating larvae with ds-Tcmof led 79.1% of the insects to arrest during its eclosion; the remaining insects died either in the larval stage or immediately following eclosion. Treating pupae with the same construct eliminated the fertility of T. castaneum. This effect was rescued by reciprocal crosses with wild-type females, but not males. We infer that the mof gene is essential for larval/pupal development and female fertility in T. castaneum.

  19. Direct and indirect genetic effects in life-history traits of flour beetles (Tribolium castaneum)

    NARCIS (Netherlands)

    Ellen, E.D.; Peeters, Katrijn; Verhoeven, Merel; Gols, Rieta; Harvey, J.A.; Wade, M.J.; Dicke, Marcel; Bijma, Piter

    2016-01-01

    Indirect genetic effects (IGEs) are the basis of social interactions among conspecifics, and can affect genetic variation of nonsocial and social traits. We used flour beetles (Tribolium castaneum) of two phenotypically distinguishable populations to estimate genetic (co)variances and the effect

  20. Tribolium castaneum as a model for high-throughput RNAi screening.

    Science.gov (United States)

    Knorr, Eileen; Bingsohn, Linda; Kanost, Michael R; Vilcinskas, Andreas

    2013-01-01

    Coleopteran insects are a highly diverse and successful order, and many beetle species are significant agricultural pests. New biorational strategies for managing populations of beetles and other insect species are needed as pests develop resistance to chemical insecticides and Bt toxins. There is now an opportunity to use genome sequence data to identify genes that are essential for insect growth, development, or survival as new targets for designing control technology. This goal requires a method for high-throughput in vivo screening of thousands of genes to identify candidate genes that, when their expression is disrupted, have a phenotype that may be useful in insect pest control. Tribolium castaneum, the red flour beetle, is a model organism that offers considerable advantages for such screening, including ease of rearing in large numbers, a sequenced genome, and a strong, systemic RNAi response for specific depletion of gene transcripts. The RNAi effect in T. castaneum can be elicited in any tissue and any stage by the injection of dsRNA into the hemocoel, and injection of dsRNA into adult females can even be used to identify phenotypes in offspring. A pilot RNAi screen (iBeetle) is underway. Several T. castaneum genes with promising RNAi phenotypes for further development as mechanisms for plant protection have been identified. These include heat shock protein 90, chitin synthase, the segmentation gene hairy, and a matrix metalloprotease. Candidate genes identified in T. castaneum screens can then be tested in agricultural pest species (in which screening is not feasible), to evaluate their effectiveness for use in potential plant-based RNAi control strategies. Delivery of dsRNA expressed by genetically modified crops to the midgut of phytophagous insects is under investigation as a new tool for very specific protection of plants from insect pest species. The T. castaneum screening platform offers a system for discovery of candidate genes with high potential

  1. Functional analysis of C1 family cysteine peptidases in the larval gut of Tenebrio molitor and Tribolium castaneum

    Science.gov (United States)

    We studied protein digestion the tenebrionids Tenebrio molitor and Tribolium castaneum, pests of stored grains and grain products, to identify potential targets for biopesticide development. Tenebrionid larvae have highly compartmentalized guts, with primarily cysteine peptidases in the acidic anter...

  2. Glutathione S-transferase (GST) genes in the red flour beetle, Tribolium castaneum, and comparative analysis with five additional insects.

    Science.gov (United States)

    Shi, Houxia; Pei, Lianghong; Gu, Shasha; Zhu, Shicheng; Wang, Yanyun; Zhang, Yi; Li, Bin

    2012-11-01

    Glutathione S-transferases are important detoxification enzymes involved in insecticide resistance. Sequencing the Tribolium castaneum genome provides an opportunity to investigate the structure, function, and evolution of GSTs on a genome-wide scale. Thirty-six putative cytosolic GSTs and 5 microsomal GSTs have been identified in T. castaneum. Furthermore, 40, 35, 13, 23, and 32 GSTs have been discovered the other insects, Drosophila, Anopheles, Apis, Bombyx, and Acyrthosiphon, respectively. Phylogenetic analyses reveal that insect-specific GSTs, Epsilon and Delta, are the largest species-specific expanded GSTs. In T. castaneum, most GSTs are tandemly arranged in three chromosomes. Particularly, Epsilon GSTs have an inverted long-fragment duplication in the genome. Other four widely distributed classes are highly conserved in all species. Given that GSTs specially expanded in Tribolium castaneum, these genes might help to resist poisonous chemical environments and produce resistance to kinds of different insecticides.

  3. Expression patterns of cysteine peptidase genes across the Tribolium castaneum life cycle provide clues to biological function.

    Science.gov (United States)

    Perkin, Lindsey; Elpidina, Elena N; Oppert, Brenda

    2016-01-01

    The red flour beetle, Tribolium castaneum, is a major agricultural pest responsible for considerable loss of stored grain and cereal products worldwide. T. castaneum larvae have a highly compartmentalized gut, with cysteine peptidases mostly in the acidic anterior part of the midgut that are critical to the early stages of food digestion. In previous studies, we described 26 putative cysteine peptidase genes in T. castaneum (types B, L, O, F, and K) located mostly on chromosomes 3, 7, 8, and 10. In the present study, we hypothesized that specific cysteine peptidase genes could be associated with digestive functions for food processing based on comparison of gene expression profiles in different developmental stages, feeding and non-feeding. RNA-Seq was used to determine the relative expression of cysteine peptidase genes among four major developmental stages (egg, larvae, pupae, and adult) of T. castaneum. We also compared cysteine peptidase genes in T. castaneum to those in other model insects and coleopteran pests. By combining transcriptome expression, phylogenetic comparisons, response to dietary inhibitors, and other existing data, we identified key cysteine peptidases that T. castaneum larvae and adults use for food digestion, and thus new potential targets for biologically-based control products.

  4. Fine-scale analysis of parasite resistance genes in the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Zhong, Daibin; Pai, Aditi; Wang, Mei-Hui; Keech, Naomi; Yan, Guiyun

    2013-09-01

    Parasite infection impacts population dynamics through effects on fitness and fecundity of the individual host. In addition to the known roles of environmental factors, host susceptibility to parasites has a genetic basis that has not been well characterized. We previously mapped quantitative trait loci (QTL) for susceptibility to rat tapeworm (Hymenolepis diminuta) infection in Tribolium castaneum using dominant AFLP markers; however, the resistance genes were not identified. Here, we refined the QTL locations and increased the marker density in the QTL regions using new microsatellite markers, sequence-tagged site markers, and single-strand conformational polymorphism markers. Resistance QTL in three linkage groups (LG3, LG6, and LG8) were each mapped to intervals castaneum resistance to H. diminuta infection.

  5. Coleoptera Associated with Decaying Wood in a Tropical Deciduous Forest.

    Science.gov (United States)

    Muñoz-López, N Z; Andrés-Hernández, A R; Carrillo-Ruiz, H; Rivas-Arancibia, S P

    2016-08-01

    Coleoptera is the largest and diverse group of organisms, but few studies are dedicated to determine the diversity and feeding guilds of saproxylic Coleoptera. We demonstrate the diversity, abundance, feeding guilds, and succession process of Coleoptera associated with decaying wood in a tropical deciduous forest in the Mixteca Poblana, Mexico. Decaying wood was sampled and classified into four stages of decay, and the associated Coleoptera. The wood was identified according to their anatomy. Diversity was estimated using the Simpson index, while abundance was estimated using a Kruskal-Wallis test; the association of Coleoptera with wood species and decay was assessed using canonical correspondence analysis. Decay wood stage I is the most abundant (51%), followed by stage III (21%). We collected 93 Coleoptera belonging to 14 families, 41 genera, and 44 species. The family Cerambycidae was the most abundant, with 29% of individuals, followed by Tenebrionidae with 27% and Carabidae with 13%. We recognized six feeding guilds. The greatest diversity of Coleoptera was recorded in decaying Acacia farnesiana and Bursera linanoe. Kruskal-Wallis analysis indicated that the abundance of Coleoptera varied according to the species and stage of decay of the wood. The canonical analysis showed that the species and stage of decay of wood determined the composition and community structure of Coleoptera. PMID:26911160

  6. Coleoptera Associated with Decaying Wood in a Tropical Deciduous Forest.

    Science.gov (United States)

    Muñoz-López, N Z; Andrés-Hernández, A R; Carrillo-Ruiz, H; Rivas-Arancibia, S P

    2016-08-01

    Coleoptera is the largest and diverse group of organisms, but few studies are dedicated to determine the diversity and feeding guilds of saproxylic Coleoptera. We demonstrate the diversity, abundance, feeding guilds, and succession process of Coleoptera associated with decaying wood in a tropical deciduous forest in the Mixteca Poblana, Mexico. Decaying wood was sampled and classified into four stages of decay, and the associated Coleoptera. The wood was identified according to their anatomy. Diversity was estimated using the Simpson index, while abundance was estimated using a Kruskal-Wallis test; the association of Coleoptera with wood species and decay was assessed using canonical correspondence analysis. Decay wood stage I is the most abundant (51%), followed by stage III (21%). We collected 93 Coleoptera belonging to 14 families, 41 genera, and 44 species. The family Cerambycidae was the most abundant, with 29% of individuals, followed by Tenebrionidae with 27% and Carabidae with 13%. We recognized six feeding guilds. The greatest diversity of Coleoptera was recorded in decaying Acacia farnesiana and Bursera linanoe. Kruskal-Wallis analysis indicated that the abundance of Coleoptera varied according to the species and stage of decay of the wood. The canonical analysis showed that the species and stage of decay of wood determined the composition and community structure of Coleoptera.

  7. Repellent Constituents of essential oil from Citrus wilsonii stem barks against Tribolium castaneum.

    Science.gov (United States)

    Wua, Yan; Chenb, Hai-Ping; Wei, Jian-Yu; Yang, Kai; Tian, Zhao-Fu; Li, Xiao-Lan; Wang, Ping-Juan; Wang, Cheng-Fang; Du, Shu-Shan; Cai, Qian

    2014-10-01

    The essential oil obtained from Citrus wilsonii Tanaka stem barks with hydrodistillation was investigated by GC-FID and GC-MS. The main components of the essential oil were identified to be nerol acetate (44.5%), nerol (13.6%), citronellyl propionate (13.5%) and α-terpineol (3.6%). Among them, the four active constituents, predicted with a bioactivity-test, were isolated and identified as nerolacetate, nerol, citronellyl propionate and α-terpineol. It was found that the essential oil of C. wilsonii stem barks possessed strong repellency (86% and 92%, respectively, at 78.6 nL/cm2, after 2 and 4 h treatment) against Tribolium castaneum adults. Repellency of the four active compounds was also determined. Nerolacetate, nerol, citronellyl propionate and α-terpineol were strongly repellent (100%, 100%, 90% and 96%, respectively, at 15.7 nL/cm2, after 2h treatment) against T. castaneum. Nerol exhibited the same level of repellency as the positive control, DEET. The results indicate that the essential oil of C. wilsonii stem barks and its active compounds have the potential to be developed as natural repellents for control of T. castaneum.

  8. Microbiota plays a role in oral immune priming in Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Momir eFuto

    2016-01-01

    Full Text Available Animals are inhabited by a diverse community of microorganisms. The relevance of such microbiota is increasingly being recognised in a broad spectrum of species, ranging from sponges to primates, revealing various beneficial roles that microbes can play. The red flour beetle Tribolium castaneum represents a well-established experimental model organism for studying questions in ecology and evolution, however, the relevance of its microbial community is still largely unknown. T. castaneum larvae orally exposed to inactivated bacterial components of the entomopathogen Bacillus thuringiensis bv. tenebrionis showed increased survival upon a subsequent challenge with spores of this bacterium. To investigate whether T. castaneum microbiota plays a role in this phenomenon, we established a protocol for raising microbe-free larvae and subsequently tested whether they differ in their ability to mount such a priming response. Here we demonstrate that larvae with significantly lowered microbial loads, show decreased survival upon secondary challenge with B. thuringiensis bv. tenebrionis spores, compared to animals which were allowed to regain their microbiota before priming. Although the exact mechanism of oral immune priming is unclear, we here suggest that microbiota plays a crucial role in oral immune priming in this species.

  9. JH modulates a cellular immunity of Tribolium castaneum in a Met-independent manner.

    Science.gov (United States)

    Hepat, Rahul; Kim, Yonggyun

    2014-04-01

    Juvenile hormone (JH) regulates diverse physiological processes in insects during entire developmental stages. Especially, the identification of Methoprene-tolerant (Met), a JH nuclear receptor, allows us to better understand molecular actions of JH to control gene expressions related with metamorphosis. However, several physiological processes including cellular immune response and some molecular actions of JH have been suspected to be mediated via its non-genomic actions. To prove its non-genomic action, JH nuclear signals were suppressed by RNA interference (RNAi) of Met or its downstream gene, Krüppel homolog 1 (Kr-h1), in the red flour beetle, Tribolium castaneum. These RNAi-treated larvae failed to undergo a normal development and suffered precocious metamorphosis. Hemocytes of T. castaneum exhibited their spreading behavior on extracellular matrix and nodule formation in response to bacterial challenge. When the larvae were treated with either RNAi of Met or Kr-h1, the hemocytes of the treated larvae were responsive to JH without any significant difference with those of control larvae. These results suggest that the response of hemocytes to JH is not mediated by its nuclear signal. On the other hand, the JH modulation of hemocyte behaviors of T. castaneum was significantly influenced by membrane and cytosolic protein activities, in which ethoxyzolamide (a specific inhibitor of carbonic anhydrase), calphostin C (a specific inhibitor of protein kinase C) or ouabain (a specific inhibitor of Na(+)-K(+) ATPase) significantly suppressed the responsiveness of hemocytes to JH.

  10. Morphological changes of condyles and Helkimo clinical dysfunction index in patients treated with Herbst--orthodontic appliance.

    Science.gov (United States)

    Aidar, Luís Antônio de Arruda; Abrahão, Márcio; Yamashita, Hélio K; Dominguez, Gladys Cristina

    2013-01-01

    This study evaluated the morphological changes in the temporomandibular joint (TMJ) condyles and calculated the Helkimo clinical dysfunction index (CDI) in adolescents with Class II Division 1 malocclusion and mandibular retrognathism treated with the Herbst appliance (phase I) and fixed orthodontic appliances (phase II). Thirty-two consecutive adolescents underwent phase I, and 23 completed phase II. The TMJs were evaluated qualitatively using magnetic resonance imaging (MRI) at the beginning of treatment (T1), during phase I (T2), at the end of phase I (T3) and at the end of phase II (T4). The CDI was calculated at T1, T3 and T4. From T1 to T3 (p=0.326), there were no changes in condyle morphology in 86.0% of the TMJs. From T3 to T4 (p<0.05) and T1 to T4 (p<0.05), changes occurred in 39.1% and 43.4% of the condyles. No significant changes in CDI occurred from T1 to T3, T3 to T4 and T1 to T4 (p=1.000; 86.6%, 76.2% and 76.2% concordance). After phase I, there were practically no changes in condyle morphology. At the end of phase II, a mild flattening was observed in some condyles. It may be concluded that no significant changes occurred in CDI after both treatment phases.

  11. Escarabeíneos copro-necrófagos (Coleoptera, Scarabaeidae, Scarabaeinae de fragmentos de Mata Atlântica em Silveira Martins, Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Pedro G. da Silva

    2012-06-01

    Full Text Available A fauna de Scarabaeinae (Coleoptera: Scarabaeidae foi amostrada através de armadilhas de queda iscadas com excremento humano e peixe apodrecido em fragmentos florestais de Silveira Martins, Rio Grande do Sul, Brasil, de novembro de 2010 a janeiro de 2011. Foi coletado um total de 1.611 indivíduos, pertencentes a seis tribos, 11 gêneros e 28 espécies. As espécies mais abundantes foram Canthon latipes Blanchard, 1845 (49,9%, C. chalybaeus Blanchard, 1845 (13,9%, Deltochilum sculpturatum Felsche, 1907 (4,9% e Eurysternus caribaeus (Herbst, 1789 (4,3%, que juntas representaram 73% do total de indivíduos capturados. As armadilhas iscadas com excremento humano capturaram maior número de espécies do que as iscadas com peixe apodrecido. Não houve diferença estatística significativa entre os tipos de iscas utilizados em relação à abundância de Scarabaeinae. A maior parte da comunidade de Scarabaeinae capturada foi representada por espécies de hábito alimentar generalista e comportamento escavador. A comunidade amostrada segue os padrões gerais de estrutura trófica e comportamental de Scarabaeinae encontrados por toda a região Neotropical.

  12. Plum curculio (Coleoptera: Curculionidae) adult mortality and associated fruit injury after exposure to field-aged insecticides on tart cherry branches.

    Science.gov (United States)

    Hoffmann, Eric J; Vandervoort, Christine; Wise, John C

    2010-08-01

    Plum curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), adults were exposed to field-aged residues of thiamethoxam, acetamiprid, thiacloprid, indoxacarb, or azinphos-methyl on tart cherry, Prunus cerasus L. variety Montmorency. At 1, 3, 7, and 14 d postapplication, fruit were sampled for chemical residues, and bioassays were used to assess beetle mortality and plant tissue injury. Azinphos-methyl had lethal activity within 1 d of exposure at all postapplication intervals and significant fruit protection extended to 14 d postapplication. All of the neonicotinoids had lethal activity at 3 d posttreatment, with acetamiprid activity extending to 7 d. Antifeedant and oviposition deterrent effects were seen with thiamethoxam and thiacloprid; damage incidence was significantly reduced in the absence of significant beetle mortality or intoxication. Thiamethoxam and acetamiprid penetrated into leaf and fruit tissue and were detected in the interior tissues at 14 d postapplication, but interior thiacloprid residues were not detected after day 1. Indoxacarb provided some fruit protection out to 7 d postapplication, and 14-d-old residues intoxicated beetles, but the slow action of this compound allowed significant damage to occur before beetles were incapacitated. Indoxacarb was only detected as a surface residue after the first day postapplication. These data on the plant-insect-chemistry interactions will support use and management decisions as compounds with acute contact activity are phased out. PMID:20857728

  13. Characterisation and tissue distribution of the PISCF allatostatin receptor in the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Audsley, Neil; Vandersmissen, Hans Peter; Weaver, Robert; Dani, Paulina; Matthews, June; Down, Rachel; Vuerinckx, Kristel; Kim, Young-Joon; Vanden Broeck, Jozef

    2013-01-01

    The insect PISCF/allatostatins (ASTs) are pleiotropic peptides that are involved in the regulation of juvenile hormone biosynthesis, are myoinhibitory on the gut and the heart, and suppress feeding in various insects, but their roles in beetles are poorly understood. To provide further insight into the significance of PISCF/ASTs in beetles, the PISCF/AST receptor from Tribolium castaneum has been characterised and its tissue distribution determined. The biological activity of the T. castaneum PISCF/AST (Trica-AS) was also investigated. The Trica-AS receptor shows high sequence homology to other insect PISCF/AST receptors, which are related to the mammalian somatostatin/opioid receptors, a family of G protein-coupled receptors. The Trica-AS receptor was activated in a dose-dependent manner by both Trica-AS and T. castaneum allatostatin double C (Trica-ASTCC) as well as Manduca sexta-allatostatin (Manse-AS). Other allatoregulatory peptides (a FLG/AST, a MIP/AST and an allatotropin) and somatostatin(14) were inactive on this receptor. Receptor transcript levels in tissues, determined by qRT-PCR, were highest in the head and the gut, with variable amounts in the fat body and reproductive organs. There were measurable differences in receptor levels of the head, fat body and reproductive organs between males and females. There was also a widespread distribution of Trica-AS in various tissues of T. castaneum. The Trica-AS peptide precursor was most abundant in the head and there was a significant difference between levels in the heads and reproductive organs of males and females. Whole mount immunocytochemistry localised Trica-AS in the median and lateral neurosecretory cells of the brain, in the corpus cardiacum and throughout the ventral nerve cord. The peptide was also present in midgut neurosecretory cells, but no immunostaining was detected in the reproductive organs or Malpighian tubules. The widespread distribution of both Trica-AS and its receptor suggest this

  14. Bioindication Potential of the Coleoptera

    Directory of Open Access Journals (Sweden)

    Belitskaya Mariya Nikolaevna

    2015-09-01

    Full Text Available Different families of Coleoptera ambiguously respond to the pollution of SPZs with industrial emissions. For example, the SPZ of Volgograd aluminum plant has the changing biodiversity of insect communities at different distances from the pollution source. The increasing level of pollution is accompanied by the reduction in species abundance. At a distance of 200 m a special ecological zone with the specific composition of the entomofauna was formed. It is significantly different from other habitats. No Cerambycidae species may survive in the zone of maximum pollution, and the number of Curculionidae species is reduced significantly. The number of Cerambycidae decreases by more than 40 % in the presence of even minimal contamination. The most sensitive bioindicators are represented by such insects as Cerambycidae, Curculionidae and Chrysomelidae. Changes in the indices can be described by the function y = arctan (x, where x is the distance from the pollution source (in meters. The specificity of this function is to identify levels of possible changes of species richness and numerical abundance of communities. On the basis of trigonometric functions describing the changes in the species composition and abundance, the authors offered the method for assessing the quality of the environment in SPZs. The use of three families of insects opens up prospects of differentiation zones of technogenic pressure.

  15. Precise and low-cost monitoring of plum curculio (Coleoptera: Curculionidae) pest activity in pyramid traps with cameras.

    Science.gov (United States)

    Selby, R D; Gage, S H; Whalon, M E

    2014-04-01

    Incorporating camera systems into insect traps potentially benefits insect phenology modeling, nonlethal insect monitoring, and research into the automated identification of traps counts. Cameras originally for monitoring mammals were instead adapted to monitor the entrance to pyramid traps designed to capture the plum curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae). Using released curculios, two new trap designs (v.I and v.II) were field-tested alongside conventional pyramid traps at one site in autumn 2010 and at four sites in autumn 2012. The traps were evaluated on the basis of battery power, ease-of-maintenance, adaptability, required-user-skills, cost (including labor), and accuracy-of-results. The v.II design fully surpassed expectations, except that some trapped curculios were not photographed. In 2012, 13 of the 24 traps recorded every curculio entering the traps during the 18-d study period, and in traps where some curculios were not photographed, over 90% of the omissions could be explained by component failure or external interference with the motion sensor. Significantly more curculios entered the camera traps between 1800 and 0000 hours. When compared with conventional pyramid traps, the v.I traps collected a similar number of curculios. Two observed but not significant trends were that the v.I traps collected twice as many plum curculios as the v.II traps, while at the same time the v.II traps collected more than twice as many photos per plum curculio as the v.I traps. The research demonstrates that low-cost, precise monitoring of field insect populations is feasible without requiring extensive technical expertise.

  16. Tribolium castaneum Apolipophorin-III acts as an immune response protein against Bacillus thuringiensis Cry3Ba toxic activity.

    Science.gov (United States)

    Contreras, Estefanía; Rausell, Carolina; Real, M Dolores

    2013-07-01

    In this study, a 2.1-fold Apolipophorin-III mRNA up-regulation was found in Tribolium castaneum larvae challenged with Bacillus thuringiensis Cry3Ba spore-crystal mixture. Knockdown of Apolipophorin-III by RNAi resulted in increased T. castaneum larvae susceptibility following Cry3Ba spore-crystal treatment, demonstrating Apolipophorin-III involvement in insect defense against B. thuringiensis. We showed that Apolipophorin-III participates in T. castaneum immune response to B. thuringiensis activating the prophenoloxidase cascade since: (i) phenoloxidase activity significantly increased after Cry3Ba spore-crystal treatment compared to untreated or Cry1Ac spore-crystal treated larvae and (ii) phenoloxidase activity in Cry3Ba spore-crystal treated Apolipophorin-III silenced larvae was 71±14% lower than that of non-silenced intoxicated larvae.

  17. Chemical Composition and Toxicity against Sitophilus zeamais and Tribolium castaneum of the Essential Oil of Murraya exotica Aerial Parts

    Directory of Open Access Journals (Sweden)

    Zhi Long Liu

    2010-08-01

    Full Text Available In our screening program for new agrochemicals from Chinese medicinal herbs, Murraya exotica was found to possess insecticidal activity against the maize weevil, Sitophilus zeamais and red flour beetle, Tribolium castaneum. The essential oil of aerial parts of M. exotica was obtained by hydrodistillation and investigated by GC and GC-MS. The main components of M. exotica essential oil were spathulenol (17.7%, a-pinene (13.3%, caryophyllene oxide (8.6%, and a-caryophyllene (7.3%. Essential oil of M. exotica possessed fumigant toxicity against S. zeamais and T. castaneum adults with LC50 values of 8.29 and 6.84 mg/L, respectively. The essential oils also show contact toxicity against S. zeamais and T. castaneum adults with LD50 values of 11.41 and 20.94 mg/adult, respectively.

  18. Evaluation of reference genes for quantitative polymerase chain reaction across life cycle stages and tissue types of Tribolium castaneum.

    Science.gov (United States)

    Toutges, Michelle J; Hartzer, Kris; Lord, Jeffrey; Oppert, Brenda

    2010-08-25

    The genome of the genetic model for coleopteran insects, Tribolium castaneum, is now available for downstream applications. To facilitate gene expression studies in T. castaneum, genes were evaluated for suitability as normalizers in comparisons across tissues and/or developmental stages. In less diverse samples, such as comparisons within developmental stages or tissue only, normalizers for mRNA were more stable and consistent. Overall, the genes for ribosomal proteins rps6, rpl13a, rps3, and rps18 were the most stable normalizers for broad scale gene expression analysis in T. castaneum. However, their stability ranking was dependent upon the instrument as well as the analysis program. These data emphasize the need to optimize normalizers used in all real-time polymerase chain reaction experiments specifically for the experimental conditions and thermocycler and to carefully evaluate data generated by computational algorithms.

  19. Gene families of cuticular proteins analogous to peritrophins (CPAPs in Tribolium castaneum have diverse functions.

    Directory of Open Access Journals (Sweden)

    Sinu Jasrapuria

    Full Text Available The functional characterization of an entire class of 17 genes from the red flour beetle, Tribolium castaneum, which encode two families of Cuticular Proteins Analogous to Peritrophins (CPAPs has been carried out. CPAP genes in T. castaneum are expressed exclusively in cuticle-forming tissues and have been classified into two families, CPAP1 and CPAP3, based on whether the proteins contain either one (CPAP1, or three copies (CPAP3 of the chitin-binding domain, ChtBD2, with its six characteristically spaced cysteine residues. Individual members of the TcCPAP1 and TcCPAP3 gene families have distinct developmental patterns of expression. Many of these proteins serve essential and non-redundant functions in maintaining the structural integrity of the cuticle in different parts of the insect anatomy. Three genes of the TcCPAP1 family and five genes of the TcCPAP3 family are essential for insect development, molting, cuticle integrity, proper locomotion or fecundity. RNA interference (RNAi targeting TcCPAP1-C, TcCPAP1-H, TcCPAP1-J or TcCPAP3-C transcripts resulted in death at the pharate adult stage of development. RNAi for TcCPAP3-A1, TcCPAP3-B, TcCPAP3-D1 or TcCPAP3-D2 genes resulted in different developmental defects, including adult/embryonic mortality, abnormal elytra or hindwings, or an abnormal 'stiff-jointed' gait. These results provide experimental support for specialization in the functions of CPAP proteins in T. castaneum and a biological rationale for the conservation of CPAP orthologs in other orders of insects. This is the first comprehensive functional analysis of an entire class of cuticular proteins with one or more ChtBD2 domains in any insect species.

  20. Effectiveness of gamma radiation for the control of tribolium castaneum, the pest of stored cashew kernels

    International Nuclear Information System (INIS)

    Effect of gamma radiation on T. castaneum irradiated during adult stage revealed that survival of adults was found to decrease with increase in radiation dose. Gamma radiations are used to produce mortality or sterility in the insects. The technique can be used by irradiating the insects at doses sufficiently high to produce the desired effects. The present study observed the gamma-ray effect on this insect, and the results may be useful for commercial applications. Overall the results showed highly significant effect of irradiation on insects administered with different doses

  1. The role of the serosa in innune defense of the beetle Tribolium castaneum

    OpenAIRE

    Bernardes, Joana Pimenta, 1988-

    2012-01-01

    Tese de mestrado. Biologia (Biologia Evolutiva e do Desenvolvimento). Universidade de Lisboa, Faculdade de Ciências, 2012 O modelo de investigação Tribolium castaneum tem sido amplamente utilizado em áreas como a biologia do desenvolvimento e evolução. Os trabalhos de investigação nesta espécie são importantes devido à sua relevância em termos económicos e de controlo de pragas. Esta espécie está inserida na ordem Coleopetera, a ordem com maior diversidade biológica conhecida. Este modelo ...

  2. Effect of cold water extracts of Acacia modesta Wall. and Glycyrrhiza glabra Linn. on Tribolium castaneum and Lemna minor.

    Science.gov (United States)

    Nazeefullah, Sayed; Dastagir, Ghulam; Ahmad, Bashir

    2014-03-01

    The aim of the present study was to introduce an alternative way for insects control through biodegradable plants materials. The different cold water extracts dilutions of Acacia modesta and Glycyrrhiza glabra were tested against Tribolium castaneum. The extracts dilutions of both plants caused mortality of the Tribolium castaneum. ANOVA revealed that dilutions and plants were highly significant. The interaction between plants and dilutions was also significant at P < 0.05. Phytotoxic activity showed that dilutions of Acacia modesta and Glycyrrhiza glabra extracts significantly inhibited the growth of Lemna minor. ANOVA showed that dilutions of both plants extracts were significant at P < 0.05.

  3. Functional analysis of the ATP-binding cassette (ABC transporter gene family of Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Broehan Gunnar

    2013-01-01

    Full Text Available Abstract Background The ATP-binding cassette (ABC transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. Most are integral membrane proteins that transport a broad spectrum of substrates across lipid membranes. In insects, ABC transporters are of special interest because of their role in insecticide resistance. Results We have identified 73 ABC transporter genes in the genome of T. castaneum, which group into eight subfamilies (ABCA-H. This coleopteran ABC family is significantly larger than those reported for insects in other taxonomic groups. Phylogenetic analysis revealed that this increase is due to gene expansion within a single clade of subfamily ABCC. We performed an RNA interference (RNAi screen to study the function of ABC transporters during development. In ten cases, injection of double-stranded RNA (dsRNA into larvae caused developmental phenotypes, which included growth arrest and localized melanization, eye pigmentation defects, abnormal cuticle formation, egg-laying and egg-hatching defects, and mortality due to abortive molting and desiccation. Some of the ABC transporters we studied in closer detail to examine their role in lipid, ecdysteroid and eye pigment transport. Conclusions The results from our study provide new insights into the physiological function of ABC transporters in T. castaneum, and may help to establish new target sites for insect control.

  4. Pharmacological and signalling properties of a D2-like dopamine receptor (Dop3) in Tribolium castaneum.

    Science.gov (United States)

    Verlinden, Heleen; Vleugels, Rut; Verdonck, Rik; Urlacher, Elodie; Vanden Broeck, Jozef; Mercer, Alison

    2015-01-01

    Dopamine is an important neurotransmitter in the central nervous system of vertebrates and invertebrates. Despite their evolutionary distance, striking parallels exist between deuterostomian and protostomian dopaminergic systems. In both, signalling is achieved via a complement of functionally distinct dopamine receptors. In this study, we investigated the sequence, pharmacology and tissue distribution of a D2-like dopamine receptor from the red flour beetle Tribolium castaneum (TricaDop3) and compared it with related G protein-coupled receptors in other invertebrate species. The TricaDop3 receptor-encoding cDNA shows considerable sequence similarity with members of the Dop3 receptor class. Real time qRT-PCR showed high expression in both the central brain and the optic lobes, consistent with the role of dopamine as neurotransmitter. Activation of TricaDop3 expressed in mammalian cells increased intracellular Ca(2+) signalling and decreased NKH-477 (a forskolin analogue)-stimulated cyclic AMP levels in a dose-dependent manner. We studied the pharmacological profile of the TricaDop3 receptor and demonstrated that the synthetic vertebrate dopamine receptor agonists, 2 - amino- 6,7 - dihydroxy - 1,2,3,4 - tetrahydronaphthalene hydrobromide (6,7-ADTN) and bromocriptine acted as agonists. Methysergide was the most potent of the antagonists tested and showed competitive inhibition in the presence of dopamine. This study offers important information on the Dop3 receptor from Tribolium castaneum that will facilitate functional analyses of dopamine receptors in insects and other invertebrates.

  5. Comparative RNA-sequencing analysis of mthl1 functions and signal transductions in Tribolium castaneum.

    Science.gov (United States)

    Li, Chengjun; Wu, Wei; Sang, Ming; Liu, Xing; Hu, Xingxing; Yun, Xiaopei; Li, Bin

    2014-09-01

    Methuselah-like 1 of Tribolium castaneum (TcMthl1) has been reported to play crucial roles in development, lifespan, stress resistance and reproduction. However, the signaling system of TcMthl1 is unknown. Thus, we compare the transcriptome profile of RNAi treated larvae (ds-Tcmthl1) and control larvae of T. castaneum by RNA-sequencing, and obtained 14,613,514 sequence reads aligned with 13,533 genes; 812 differentially expressed genes (DEGs) were identified. These DEGs were classified into 47 GO functional groups, including such functions as the immune system process, the response to stimulus, the developmental process and reproduction. Interestingly, knock-down of Tcmthl1 suppressed both of Toll and IMD immunity pathways which most likely modulated the effects of Tcmthl1 on lifespan and stress resistance. Additionally, the DEGs encoding Blimp-1, Ftz-F1, E74 and Timeless may participate in the development and reproduction of ds-Tcmthl1 insects. The findings of these DEGs and pathways will provide valuable insight into TcMthl1 signaling and regulation system.

  6. 基于28S rRNA基因的PCR-RFLP分析对赤拟谷盗与杂拟谷盗进行分子鉴定%Molecular identification of Tribolium castaneum and T.confusum based on PCR-RFLP analyses of 28S rRNA gene

    Institute of Scientific and Technical Information of China (English)

    张汉松; 冯照军; 程超

    2014-01-01

    本研究拟利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析方法对赤拟谷盗Tribolium castaneum (Herbst)和杂拟谷盗Tribolium confusum(Jac du Val)进行分子鉴定,以期为仓储害虫管理和口岸检疫提供技术帮助和支持.采用通用引物对赤拟谷盗和杂拟谷盗的28S rRNA基因进行了PCR扩增、序列测定和分析,结果发现:扩增片段长约1070 bp,该序列种内均无变异位点、种间有76个变异位点,即种内没有核苷酸替换发生、种间核苷酸替换发生76次,其中转换56次,颠换20次,转换/颠换的比值为2.80.用限制性内切酶PvuⅠ对赤拟谷盗和杂拟谷盗的28S rRNA基因扩增产物进行酶切,电泳检测显示,赤拟谷盗和杂拟谷盗的28S rRNA基因扩增产物的PvuⅠ酶切图谱(分别产生2个和3个酶切条带)明显不同,因此本研究建立的28SrRNA基因PCR-RFLP方法可用于赤拟谷盗与杂拟谷盗的分子鉴定.

  7. Functions of Ion Transport Peptide and Ion Transport Peptide-Like in the Red Flour Beetle Tribolium castaneum

    Science.gov (United States)

    Ion transport peptide (ITP) and ITP-like (ITPL) are highly conserved neuropeptides in insects and crustaceans. We investigated the alternatively spliced variants of ITP/ITPL in Tribolium castaneum to understand their functions. We identified three alternatively spliced transcripts named itp, itpl-...

  8. Identification and characterization of novel ER-based hsp90 gene in the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Zhang, Yi; Gu, Shasha; Li, Chengjun; Sang, Ming; Wu, Wei; Yun, Xiaopei; Hu, Xingxing; Li, Bin

    2014-09-01

    Heat-shock protein 90 (HSP90) is a highly conserved molecular chaperone found in all species except for Archaea, which is required not only for stress tolerance but also for normal development. Recently, it was reported that HSP83, one member of the cytosolic HSP90 family, contributes to oogenesis and responds to heat resistance in Tribolium castaneum. Here, a novel ER-based HSP90 gene, Tchsp90, has been identified in T. castaneum. Phylogenetic analysis showed that hsp90s and hsp83s evolved separately from a common ancestor but that hsp90s originated earlier. Quantitative real-time polymerase chain reaction illustrated that Tchsp90 is expressed in all developmental stages and is highly expressed at early pupa and late adult stages. Tchsp90 was upregulated in response to heat stress but not to cold stress. Laval RNAi revealed that Tchsp90 is important for larval/pupal development. Meanwhile, parental RNAi indicated that it completely inhibited female fecundity and partially inhibited male fertility once Tchsp90 was knocked down and that it will further shorten the lifespan of T. castaneum. These results suggest that Tchsp90 is essential for development, lifespan, and reproduction in T. castaneum in addition to its response to heat stress.

  9. Estudo cefalométrico das alterações dentoesqueléticas da má oclusão de Classe II, divisão 1 tratada com o aparelho de Herbst com cantiléver Cephalometric study in patients that displayed Class II, division 1 malocclusion treated with herbst appliance

    OpenAIRE

    Ana Carla Raphaelli Nahás; José Fernando Castanha Henriques; Guilherme Janson; Bryan D. Tompson; Donald G. Woodside

    2008-01-01

    OBJETIVO: Realizou-se um estudo cefalométrico, em telerradiografias, objetivando-se determinar os efeitos no complexo craniofacial de pacientes com más oclusões de Classe II, divisão 1 submetidos ao tratamento com o aparelho de Herbst com cantiléver. METODOLOGIA: Para tanto, utilizou-se uma amostra composta por dois grupos, sendo um experimental e um controle. O grupo experimental originou-se da Faculdade de Odontologia de Bauru - USP, com 25 pacientes tratados com o aparelho ortopédico funci...

  10. ON THE TAXONOMY AND NOMENCLATURE OF SOME MECININI (COLEOPTERA, CURCULIONIDAE

    Directory of Open Access Journals (Sweden)

    Roberto Caldara

    2008-04-01

    Full Text Available In accordance with the Code, ten actions are taken to preserve nomenclatural stability of names of taxa currently belonging to Mecinini. Following the provisions of ICZN Article 23.9.1, Cleopomiarus graminis (Gyllenhal, 1813 (formerly Rhynchaenus is made a nomen protectum and Curculio ellipticus Herbst, 1795 is made a nomen oblitum; Rhinusa antirrhini (Paykull, 1800 (formerly Curculio is made a nomen protectum and Curculio noctis herbst, 1795 is made a nomen oblitum; having met the conditions of ICZN article 75.3 the neotypes of the following taxa are designated: Curculio antirrhini Paykull, 1800, Curculio cinctus Rossi, 1790, Curculio curvirostris Rossi, 1790, Curculio linariae Panzer, 1792, Cionus thapsicola Germar, 1821, Mecinus collaris Germar, 1821. Lectotypes of Curculio ellipticus Herbst, 1795, Gymnetron eversmanni Rosenschöld, 1838, Mecinus barbarus Gyllenhal, 1838, and Mecinus longiusculus Boheman, 1845 are also designated. Rhinusa linariae (Panzer, 1792 (formerly Curculio remains the valid name of the taxon since Curculio curvirostris Rossi, 1790 (non Fabricius, 1781 nec Herbst, 1784 is unavailable; Mecinus collaris Germar, 1821 remains the valid name of the taxon since Curculio cinctus Rossi, 1790 (non Drury, 1782 nec Geoffroy, 1785 is unavailable. The following new synonymies are proposed: Mecinus barbarus Gyllenhal, 1838 = Mecinus longiusculus Boheman, 1845 n. syn., = Mecinus teretiusculus Boheman, 1845 n. syn., = Mecinus filiformis Aubé, 1850 n. syn.; Rhinusa florum (Rübsaamen, 1895 = Gymnetron smreczynskii Fremuth, 1972 n. syn.; Rhinusa tetra (Fabricius, 1792 = Cionus thapsicola Germar, 1821 n. syn. Rhinusa eversmanni (Rosenschöld, 1838 is the name proposed for Rhinusa thapsicola sensu auctorum (non Germar, 1821.

  11. Effective and Efficient Herbst Appliance Therapy for Skeletal Class II Malocclusion Patient with a Low Degree of Collaboration with the Orthodontic Treatment

    Directory of Open Access Journals (Sweden)

    Bernardo Quiroga Souki

    2015-01-01

    Full Text Available The current concept for effective and efficient treatment of skeletal Class II malocclusion prescribes that interceptive approach should be delivered during the pubertal growth stage. However, psychosocial issues and a greater risk of dental trauma are also factors that should be addressed when considering early Class II therapy. This paper reports a case of a patient that sought orthodontic treatment due to aesthetic discomfort with the incisors’ protrusion. Two previous treatments failed because patient’s collaboration with removable appliances was inadequate. Given his history of no collaboration and because the patient was in the prepubertal stage, it was decided to try a different approach in the third attempt of treatment. Traumatic injury protective devices were used during the prepubertal stage and followed by Herbst appliance and fixed multibrackets therapy during the pubertal stage, resulting in an adequate outcome and long-term stability.

  12. Live imaging of Tribolium castaneum embryonic development using light-sheet-based fluorescence microscopy.

    Science.gov (United States)

    Strobl, Frederic; Schmitz, Alexander; Stelzer, Ernst H K

    2015-10-01

    Tribolium castaneum has become an important insect model organism for evolutionary developmental biology, genetics and biotechnology. However, few protocols for live fluorescence imaging of Tribolium have been reported, and little image data is available. Here we provide a protocol for recording the development of Tribolium embryos with light-sheet-based fluorescence microscopy. The protocol can be completed in 4-7 d and provides procedural details for: embryo collection, microscope configuration, embryo preparation and mounting, noninvasive live imaging for up to 120 h along multiple directions, retrieval of the live embryo once imaging is completed, and image data processing, for which exemplary data is provided. Stringent quality control criteria for developmental biology studies are also discussed. Light-sheet-based fluorescence microscopy complements existing toolkits used to study Tribolium development, can be adapted to other insect species, and requires no advanced imaging or sample preparation skills.

  13. A system to efficiently maintain embryonic lethal mutations in the flour beetle Tribolium castaneum.

    Science.gov (United States)

    Berghammer, A; Bucher, G; Maderspacher, F; Klingler, M

    1999-06-01

    Due to its small size, short life cycle, and easy maintenance, the flour beetle Tribolium castaneum is well suited for the genetic analysis of development. One drawback of Tribolium as a genetic system is, however, the difficulty of keeping embryonic lethal lines. Presently, only few lethal mutations can be kept as balanced stocks. Therefore, heterozygous carriers must be identified anew in every generation in order to maintain a recessive embryonic mutation. To alleviate this problem we have devised a block system that allows the simultaneous processing of many mutant lines or test crosses for visual inspection of larval cuticle phenotypes. Using this technique, one person can maintain about 100 embryonic lethal stocks, which makes feasible the thorough genetic analysis of embryogenesis in this species.

  14. Genomics, transcriptomics, and peptidomics of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum

    DEFF Research Database (Denmark)

    Li, Bin; Predel, Reinhard; Neupert, Susanne;

    2008-01-01

    Neuropeptides and protein hormones are ancient molecules that mediate cell-to-cell communication. The whole genome sequence from the red flour beetle Tribolium castaneum, along with those from other insect species, provides an opportunity to study the evolution of the genes encoding neuropeptide...... and protein hormones. We identified 41 of these genes in the Tribolium genome by using a combination of bioinformatic and peptidomic approaches. These genes encode >80 mature neuropeptides and protein hormones, 49 peptides of which were experimentally identified by peptidomics of the central nervous system...... with a sequenced genome. The presence of many additional osmoregulatory peptides in Tribolium agrees well with its ability to live in very dry surroundings. In contrast to these extra genes, there are at least nine neuropeptide genes missing in Tribolium, including the genes encoding the prepropeptides...

  15. Genome-wide mapping of conserved microRNAs and their host transcripts in Tribolium castaneum

    Institute of Scientific and Technical Information of China (English)

    Qibin Luo; Qing Zhou; Xiaomin Yu; Hongbin Lin; Songnian Hu; Jun Yu

    2008-01-01

    MicroRNAs (miRNAs) are endogenous 22-nt RNAs, which play important regulatory roles by post-transcriptional gene silencing. A computational strategy has been developed for the identification of conserved miRNAs based on features of known metazoan miRNAs in red flour beetle (Tribolium castaneum), which is regarded as one of the major laboratory models of arthropods. Among 118 putative miRNAs, 47% and 53% of the predicted miRNAs from the red flour beetle are harbored by known protein-coding genes (intronic) and genes located outside (intergenic miRNA), respectively. There are 31 intronic miRNAs in the same transcriptional orientation as the host genes, which may share RNA polymerase Ⅱ and spliceosomal machinery with their host genes for their biogenesis. A hypothetical feedback model has been proposed based on the analysis of the relationship between intronic miRNAs and their host genes in the development of red flour beetle.

  16. MicroRNA evolution, expression, and function during short germband development in Tribolium castaneum.

    Science.gov (United States)

    Ninova, Maria; Ronshaugen, Matthew; Griffiths-Jones, Sam

    2016-01-01

    MicroRNAs are well-established players in the development of multicellular animals. Most of our understanding of microRNA function in arthropod development comes from studies in Drosophila. Despite their advantages as model systems, the long germband embryogenesis of fruit flies is an evolutionary derived state restricted to several holometabolous insect lineages. MicroRNA evolution and expression across development in animals exhibiting the ancestral and more widespread short germband mode of embryogenesis has not been characterized. We sequenced small RNA libraries of oocytes and successive intervals covering the embryonic development of the short germband model organism, Tribolium castaneum. We analyzed the evolution and temporal expression of the microRNA complement and sequenced libraries of total RNA to investigate the relationships with microRNA target expression. We show microRNA maternal loading and sequence-specific 3' end nontemplate oligoadenylation of maternally deposited microRNAs that is conserved between Tribolium and Drosophila. We further uncover large clusters encoding multiple paralogs from several Tribolium-specific microRNA families expressed during a narrow interval of time immediately after the activation of zygotic transcription. These novel microRNAs, together with several early expressed conserved microRNAs, target a significant number of maternally deposited transcripts. Comparison with Drosophila shows that microRNA-mediated maternal transcript targeting is a conserved process in insects, but the number and sequences of microRNAs involved have diverged. The expression of fast-evolving and species-specific microRNAs in the early blastoderm of T. castaneum is consistent with previous findings in Drosophila and shows that the unique permissiveness for microRNA innovation at this stage is a conserved phenomenon.

  17. Satellite DNA Modulates Gene Expression in the Beetle Tribolium castaneum after Heat Stress.

    Science.gov (United States)

    Feliciello, Isidoro; Akrap, Ivana; Ugarković, Đurđica

    2015-08-01

    Non-coding repetitive DNAs have been proposed to perform a gene regulatory role, however for tandemly repeated satellite DNA no such role was defined until now. Here we provide the first evidence for a role of satellite DNA in the modulation of gene expression under specific environmental conditions. The major satellite DNA TCAST1 in the beetle Tribolium castaneum is preferentially located within pericentromeric heterochromatin but is also dispersed as single repeats or short arrays in the vicinity of protein-coding genes within euchromatin. Our results show enhanced suppression of activity of TCAST1-associated genes and slower recovery of their activity after long-term heat stress relative to the same genes without associated TCAST1 satellite DNA elements. The level of gene suppression is not influenced by the distance of TCAST1 elements from the associated genes up to 40 kb from the genes' transcription start sites, but it does depend on the copy number of TCAST1 repeats within an element, being stronger for the higher number of copies. The enhanced gene suppression correlates with the enrichment of the repressive histone marks H3K9me2/3 at dispersed TCAST1 elements and their flanking regions as well as with increased expression of TCAST1 satellite DNA. The results reveal transient, RNAi based heterochromatin formation at dispersed TCAST1 repeats and their proximal regions as a mechanism responsible for enhanced silencing of TCAST1-associated genes. Differences in the pattern of distribution of TCAST1 elements contribute to gene expression diversity among T. castaneum strains after long-term heat stress and might have an impact on adaptation to different environmental conditions.

  18. Satellite DNA Modulates Gene Expression in the Beetle Tribolium castaneum after Heat Stress.

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    Isidoro Feliciello

    2015-08-01

    Full Text Available Non-coding repetitive DNAs have been proposed to perform a gene regulatory role, however for tandemly repeated satellite DNA no such role was defined until now. Here we provide the first evidence for a role of satellite DNA in the modulation of gene expression under specific environmental conditions. The major satellite DNA TCAST1 in the beetle Tribolium castaneum is preferentially located within pericentromeric heterochromatin but is also dispersed as single repeats or short arrays in the vicinity of protein-coding genes within euchromatin. Our results show enhanced suppression of activity of TCAST1-associated genes and slower recovery of their activity after long-term heat stress relative to the same genes without associated TCAST1 satellite DNA elements. The level of gene suppression is not influenced by the distance of TCAST1 elements from the associated genes up to 40 kb from the genes' transcription start sites, but it does depend on the copy number of TCAST1 repeats within an element, being stronger for the higher number of copies. The enhanced gene suppression correlates with the enrichment of the repressive histone marks H3K9me2/3 at dispersed TCAST1 elements and their flanking regions as well as with increased expression of TCAST1 satellite DNA. The results reveal transient, RNAi based heterochromatin formation at dispersed TCAST1 repeats and their proximal regions as a mechanism responsible for enhanced silencing of TCAST1-associated genes. Differences in the pattern of distribution of TCAST1 elements contribute to gene expression diversity among T. castaneum strains after long-term heat stress and might have an impact on adaptation to different environmental conditions.

  19. Tribolium castaneum Transformer-2 regulates sex determination and development in both males and females.

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    Shukla, Jayendra Nath; Palli, Subba Reddy

    2013-12-01

    Tribolium castaneum Transformer (TcTra) is essential for female sex determination and maintenance through the regulation of sex-specific splicing of doublesex (dsx) pre-mRNA. In females, TcTra also regulates the sex-specific splicing of its own pre-mRNA to ensure continuous production of functional Tra protein. Transformer protein is absent in males and hence dsx pre-mRNA is spliced in a default mode. The mechanisms by which males inhibit the production of functional Tra protein are not known. Here, we report on functional characterization of transformer-2 (tra-2) gene (an ortholog of Drosophila transformer-2) in T. castaneum. RNA interference-mediated knockdown in the expression of gene coding for tra-2 in female pupae or adults resulted in the production of male-specific isoform of dsx and both female and male isoforms of tra suggesting that Tra-2 is essential for the female-specific splicing of tra and dsx pre-mRNAs. Interestingly, knockdown of tra-2 in males did not affect the splicing of dsx but resulted in the production of both female and male isoforms of tra suggesting that Tra-2 suppresses female-specific splicing of tra pre-mRNA in males. This dual regulation of sex-specific splicing of tra pre-mRNA ensures a tight regulation of sex determination and maintenance. These data suggest a critical role for Tra-2 in suppression of female sex determination cascade in males. In addition, RNAi studies showed that Tra-2 is also required for successful embryonic and larval development in both sexes.

  20. O escaravelho Megasoma gyas (Herbst, 1775, espécie ameaçada de extinção, no Parque Estadual Carlos Botelho, Sete Barras – SP. The elephant beetle Megasoma gyas (Herbst, 1775, threatened species, in Carlos Botelho State Park, Sete Barras, SP, Brazil.

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    Alexsander Zamorano ANTUNES

    2007-12-01

    Full Text Available As espécies do gênero Megasoma sãobesouros naturalmente raros e pouco conhecidos.Megasoma gyas (Herbst, 1775, espécie restrita aoterritório brasileiro e considerada ameaçada deextinção, foi encontrada no Parque Estadual CarlosBotelho em maio de 2007. O presente trabalhorevisa a distribuição da espécie no Estado de SãoPaulo, detalha a sua simpatria com outra espéciedo gênero e discute aspectos comportamentais eameaças à sua sobrevivência.The elephant beetle Megasoma spp. arenaturally scarce and poorly known species.Megasoma gyas (Herbst, 1775, Brazilian endemicand threatened species, was recorded in CarlosBotelho State Park, in May 2007. The speciesdistribution in the State of São Paulo, southeasternBrazil, is reviewed and the simpatry with anotherMegasoma species, behavioral aspects and threatsfaced by this species are shown.

  1. PDP1 regulates energy metabolism through the IIS-TOR pathway in the red flour beetle, Tribolium castaneum.

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    Li, Zhiqian; Jiang, Jianhao; Chen, Yazhou; You, Lang; Huang, Yongping; Tan, Anjiang; Li, Zhiqian; Jiang, Jianhao; Niu, Baolong; Meng, Zhiqi

    2014-03-01

    The PAR-domain protein 1 (PDP1) is essential for locomotor activity of insects. However, its functions in insect growth and development have not been studied extensively, which prompted our hypothesis that PDP1 acts in energy metabolism. Here we report identification of TcPDP1 in the red flour beetle, Tribolium castaneum, and its functional analysis by RNAi. Treating larvae with dsTcPDP1 induced pupae developmental arrestment, accompanied by accelerated fat body degradation. dsTcPDP1 treatments in adults resulted in reduced female fecundity. Disruption of TcPDP1 expression affected the transcription of genes involved in insulin signaling transduction and mechanistic target of rapamycin (mTOR) pathway. These results support our hypothesis that TcPDP1 acts in energy metabolism in T. castaneum.

  2. Diagnostic molecular markers for phosphine resistance in U.S. populations of Tribolium castaneum and Rhyzopertha dominica.

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    Chen, Zhaorigetu; Schlipalius, David; Opit, George; Subramanyam, Bhadriraju; Phillips, Thomas W

    2015-01-01

    Stored product beetles that are resistant to the fumigant pesticide phosphine (hydrogen phosphide) gas have been reported for more than 40 years in many places worldwide. Traditionally, determination of phosphine resistance in stored product beetles is based on a discriminating dose bioassay that can take up to two weeks to evaluate. We developed a diagnostic cleaved amplified polymorphic sequence method, CAPS, to detect individuals with alleles for strong resistance to phosphine in populations of the red flour beetle, Tribolium castaneum, and the lesser grain borer, Rhyzopertha dominica, according to a single nucleotide mutation in the dihydrolipoamide dehydrogenase (DLD) gene. We initially isolated and sequenced the DLD genes from susceptible and strongly resistant populations of both species. The corresponding amino acid sequences were then deduced. A single amino acid mutation in DLD in populations of T. castaneum and R. dominica with strong resistance was identified as P45S in T. castaneum and P49S in R. dominica, both collected from northern Oklahoma, USA. PCR products containing these mutations were digested by the restriction enzymes MboI and BstNI, which revealed presence or absence, respectively of the resistant (R) allele and allowed inference of genotypes with that allele. Seven populations of T. castaneum from Kansas were subjected to discriminating dose bioassays for the weak and strong resistance phenotypes. Application of CAPS to these seven populations confirmed the R allele was in high frequency in the strongly resistant populations, and was absent or at a lower frequency in populations with weak resistance, which suggests that these populations with a low frequency of the R allele have the potential for selection of the strong resistance phenotype. CAPS markers for strong phosphine resistance will help to detect and confirm resistant beetles and can facilitate resistance management actions against a given pest population.

  3. Diagnostic molecular markers for phosphine resistance in U.S. populations of Tribolium castaneum and Rhyzopertha dominica.

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    Zhaorigetu Chen

    Full Text Available Stored product beetles that are resistant to the fumigant pesticide phosphine (hydrogen phosphide gas have been reported for more than 40 years in many places worldwide. Traditionally, determination of phosphine resistance in stored product beetles is based on a discriminating dose bioassay that can take up to two weeks to evaluate. We developed a diagnostic cleaved amplified polymorphic sequence method, CAPS, to detect individuals with alleles for strong resistance to phosphine in populations of the red flour beetle, Tribolium castaneum, and the lesser grain borer, Rhyzopertha dominica, according to a single nucleotide mutation in the dihydrolipoamide dehydrogenase (DLD gene. We initially isolated and sequenced the DLD genes from susceptible and strongly resistant populations of both species. The corresponding amino acid sequences were then deduced. A single amino acid mutation in DLD in populations of T. castaneum and R. dominica with strong resistance was identified as P45S in T. castaneum and P49S in R. dominica, both collected from northern Oklahoma, USA. PCR products containing these mutations were digested by the restriction enzymes MboI and BstNI, which revealed presence or absence, respectively of the resistant (R allele and allowed inference of genotypes with that allele. Seven populations of T. castaneum from Kansas were subjected to discriminating dose bioassays for the weak and strong resistance phenotypes. Application of CAPS to these seven populations confirmed the R allele was in high frequency in the strongly resistant populations, and was absent or at a lower frequency in populations with weak resistance, which suggests that these populations with a low frequency of the R allele have the potential for selection of the strong resistance phenotype. CAPS markers for strong phosphine resistance will help to detect and confirm resistant beetles and can facilitate resistance management actions against a given pest population.

  4. Evolutionary variations in the expression of dorso-ventral patterning genes and the conservation of pioneer neurons in Tribolium castaneum.

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    Biffar, Lucia; Stollewerk, Angelika

    2015-04-01

    Insects are ideally suited for gaining insight into the evolutionary developmental mechanisms that have led to adaptive changes of the nervous system since the specific structure of the nervous system can be directly linked to the neural stem cell (neuroblast) lineages, which in turn can be traced back to the last common ancestor of insects. The recent comparative analysis of the Drosophila melanogaster and Tribolium castaneum neuroblast maps revealed substantial differences in the expression profiles of neuroblasts. Here we show that despite the overall conservation of the dorso-ventral expression domains of muscle segment homeobox, intermediate neuroblasts defective and ventral nervous system defective, the expression of these genes relative to the neuroblasts in the respective domains has changed considerably during insect evolution. Furthermore, functional studies show evolutionary changes in the requirement of ventral nervous system defective in the formation of neuroblast 1-1 and the correct differentiation of its presumptive progeny, the pioneer neurons aCC and pCC. The inclusion of the expression data of the dorso-ventral genes into the recently established T. castaneum neuroblast map further increases the differences in the neuroblast expression profiles between D. melanogaster and T. castaneum. Despite these molecular variations, the Even-skipped positive pioneer neurons show an invariant arrangement, except for an additional Even-skipped positive cluster that we discovered in T. castaneum. Given the importance of these pioneer neurons in establishing the intersegmental nerves and the longitudinal tracts, which are part of the conserved axonal scaffold of arthropods, we discuss internal buffering mechanisms that might ensure that neuroblast lineages invariantly generate pioneer neurons over a wide range of molecular variations.

  5. Glycogen and glucose metabolism are essential for early embryonic development of the red flour beetle Tribolium castaneum.

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    Fraga, Amanda; Ribeiro, Lupis; Lobato, Mariana; Santos, Vitória; Silva, José Roberto; Gomes, Helga; da Cunha Moraes, Jorge Luiz; de Souza Menezes, Jackson; de Oliveira, Carlos Jorge Logullo; Campos, Eldo; da Fonseca, Rodrigo Nunes

    2013-01-01

    Control of energy metabolism is an essential process for life. In insects, egg formation (oogenesis) and embryogenesis is dependent on stored molecules deposited by the mother or transcribed later by the zygote. In oviparous insects the egg becomes an isolated system after egg laying with all energy conversion taking place during embryogenesis. Previous studies in a few vector species showed a strong correlation of key morphogenetic events and changes in glucose metabolism. Here, we investigate glycogen and glucose metabolism in the red flour beetle Tribolium castaneum, an insect amenable to functional genomic studies. To examine the role of the key enzymes on glycogen and glucose regulation we cloned and analyzed the function of glycogen synthase kinase 3 (GSK-3) and hexokinase (HexA) genes during T. castaneum embryogenesis. Expression analysis via in situ hybridization shows that both genes are expressed only in the embryonic tissue, suggesting that embryonic and extra-embryonic cells display different metabolic activities. dsRNA adult female injection (parental RNAi) of both genes lead a reduction in egg laying and to embryonic lethality. Morphological analysis via DAPI stainings indicates that early development is impaired in Tc-GSK-3 and Tc-HexA1 RNAi embryos. Importantly, glycogen levels are upregulated after Tc-GSK-3 RNAi and glucose levels are upregulated after Tc-HexA1 RNAi, indicating that both genes control metabolism during embryogenesis and oogenesis, respectively. Altogether our results show that T. castaneum embryogenesis depends on the proper control of glucose and glycogen.

  6. Penetration through the peritrophic matrix is a key to lectin toxicity against Tribolium castaneum.

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    Walski, Tomasz; Van Damme, Els J M; Smagghe, Guy

    2014-11-01

    In the last decades lectins have received a lot of attention as potential tools in pest control. Despite substantial progress in the field not all the factors determining insecticidal potency and selectivity of these proteins have been described. Recently, three lectins, RSA (Rhizoctonia solani agglutinin), SNA-I and SNA-II (Sambucus nigra agglutinin I and II) have been shown to be toxic to aphids and caterpillars. In this project we investigated if these lectins are also toxic against larvae and a cell line of the red flour beetle, Tribolium castaneum, a model organism and important pest of stored products. Furthermore, we analyzed the stability of the lectins in the larval gut and used confocal microscopy to compare their efficiency in passing through the peritrophic matrix (PM). We observed that all three lectins were toxic against the T. castaneum cell line and their effectiveness in vitro was in decreasing order SNA-II>SNA-I>RSA with the respective EC50 being 0.1, 0.5 and 3.6 μg/ml. Larvae feeding for 16 day on diets containing 2% RSA, 2% SNA-II and 2% SNA-I weighed 0.14 ± 0.07 mg, 0.67 ± 0.44 mg and 1.89 ± 0.38 mg, corresponding to approximately 7%, 36% and 80% of control larvae, respectively. As a consequence, RSA increased the time to adult emergence by over 3-fold, SNA-II by 1.9-fold and SNA-I by 1.2-fold. RSA and SNA-II were stable in the larval gut, while SNA-I was digested and excreted with the feces. Finally, confocal microscopy confirmed that RSA passed through the PM more efficiently than SNA-II. In conclusion, our data suggest that the lectin ability to pass through the PM, governed by molecule dimensions, charge and size of PM pores, is one of the features that determine the toxicity of these insecticidal proteins.

  7. Estudo cefalométrico das alterações dentoesqueléticas da má oclusão de Classe II, divisão 1 tratada com o aparelho de Herbst com cantiléver Cephalometric study in patients that displayed Class II, division 1 malocclusion treated with herbst appliance

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    Ana Carla Raphaelli Nahás

    2008-02-01

    Full Text Available OBJETIVO: Realizou-se um estudo cefalométrico, em telerradiografias, objetivando-se determinar os efeitos no complexo craniofacial de pacientes com más oclusões de Classe II, divisão 1 submetidos ao tratamento com o aparelho de Herbst com cantiléver. METODOLOGIA: Para tanto, utilizou-se uma amostra composta por dois grupos, sendo um experimental e um controle. O grupo experimental originou-se da Faculdade de Odontologia de Bauru - USP, com 25 pacientes tratados com o aparelho ortopédico funcional e a idade inicial média de 12,01 anos. O grupo de controle, pareado cronologicamente ao grupo experimental, foi composto por pacientes não tratados ortodonticamente e/ou ortopedicamente, oriundos do arquivo de documentações denominado Burlington Growth Centre, localizado na Faculdade de Odontologia da Universidade de Toronto, Canadá. Para cada componente dos dois grupos, obtiveram-se as telerradiografias ao início (T1 e ao final (T2 do período de tratamento ou de observação, sendo traçadas manualmente e digitalizadas para um programa de cefalometria. Foram estabelecidas 33 grandezas cefalométricas. RESULTADOS E CONCLUSÕES: A comparação estatística entre o grupo experimental e o grupo controle (teste t de Student, com nível de significância pOBJECTIVE: This study investigated the treatment effects on the craniofacial growth of Class II patients treated with Herbst appliance. METHODOLOGY: The sample was comprised of two groups, one experimental and one control group. The experimental group originated from Bauru Dental School, University of São Paulo. This consisted of 25 patients treated by the Herbst appliance, with an initial mean age of 12,01 years. The control group, matched by age with the experimental group, was originated from the Burlington Growth Centre, located at the Faculty of Dentistry, University of Toronto, Canada. All patients were assessed at the beginning (T1 and at the end of the treatment or observation period

  8. Insulin receptor regulates food intake through sulfakinin signaling in the red flour beetle, Tribolium castaneum.

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    Lin, Xianyu; Yu, Na; Smagghe, Guy

    2016-06-01

    Insects obtain energy and nutrients via feeding to support growth and development. The insulin signaling pathway is involved in the regulation of feeding; however, the underlying mechanisms are not fully understood. Here, we show that insulin signaling regulates food intake via crosstalk with neuropeptide sulfakinin in the red flour beetle, Tribolium castaneum. Silencing of the insulin receptor (InR) decreased the food intake in the penultimate and final instar stages, leading to a decrease of weight gain and mortality during larval-pupal metamorphosis. Interestingly, the knockdown of InR co-occurred with an increased expression of sulfakinin (sk), a gene encoding neuropeptide SK functioning as a satiety signal. In parallel, double silencing of sk and InR eliminated the inhibitory effect on food intake as induced by silencing of InR and the larvae died as prepupae. In conclusion, this study shows, for the first time, that the insulin/InR signaling regulates food intake through the sulfakinin signaling pathway in the larval stages of this important model and pest insect, indicating a novel target for pest control. PMID:26972481

  9. The Tribolium castaneum cell line TcA: a new tool kit for cell biology.

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    Silver, Kristopher; Jiang, Hongbo; Fu, Jinping; Phillips, Thomas W; Beeman, Richard W; Park, Yoonseong

    2014-10-30

    The red flour beetle, Tribolium castaneum, is an agriculturally important insect pest that has been widely used as a model organism. Recently, an adherent cell line (BCIRL-TcA-CLG1 or TcA) was developed from late pupae of the red flour beetle. Next generation transcriptome sequencing of TcA cells demonstrated expression of a wide variety of genes associated with specialized functions in chitin metabolism, immune responses and cellular and systemic RNAi pathways. Accordingly, we evaluated the sensitivity of TcA cells to dsRNA to initiate an RNAi response. TcA cells were highly sensitive to minute amounts of dsRNA, with a minimum effective dose of 100 pg/mL resulting in significant suppression of gene expression. We have also developed a plasmid containing two TcA-specific promoters, the promoter from the 40S ribosomal protein subunit (TC006550) and a bi-directional heat shock promoter (TcHS70) from the intergenic space between heat shock proteins 68a and b. These promoters have been employed to provide high levels of either constitutive (TC006550) or inducible (TcHS70) gene expression of the reporter proteins. Our results show that the TcA cell line, with its sensitivity to RNAi and functional TcA-specific promoters, is an invaluable resource for studying basic molecular and physiological questions.

  10. Effect of selection and gamma-irradiation on fecundity in Tribolium castaneum

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    Bhat, P.P.; Raheja, K.L. (Indian Veterinary Research Inst., Izatnagar, Uttar Pradesh); Bhat, P.N. (Central Inst. for Research on Goats, Mathura (India)); Verma, S.B. (Rajendra Agricultural Univ., Patna (India))

    1981-10-01

    In order to investigate the joint effect of selection and gamma irradiation on a polygenic trait like egg production in Tribolium castaneum, a 3 x 3 x 2 fractorial experiment with 3 levels of selection, viz. O, high (S/sub 1/), and low (S/sub 2/); 3 levels of irradiation, viz. 0,1 1500R (R/sub 1/) 2300R(R/sub 2/); and 2 replicates with 24 females in each cell, was set up. The data were collected on 721 females of parental generation subjected to selection and radiation and the subsequent progeny generation. A significant divergence of 11.6 eggs were observed due to high and low selection methods. There was no difference between control and R/sub 1/ radiation dose. The R/sub 2/ radiation dose depressed the egg production of the progeny significantly. There was a linear decrease in egg number in the progeny with an increase in dose level of radiation in parents; this is indicative of significant number of gene mutations effecting a quantitative trait like egg production. It is suggested that reduction in fecundity in the progeny of parents subjected to gamma-irradiation can possibly arise due to either (i) molecular rearrangement at a point on the chromosome leading to gene mutation or (ii) chromosome breakage with subsequent rearrangement of gene position or both.

  11. Satellite DNA as a driver of population divergence in the red flour beetle Tribolium castaneum.

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    Feliciello, Isidoro; Akrap, Ivana; Brajković, Josip; Zlatar, Ivo; Ugarković, Đurđica

    2014-12-19

    Tandemly repeated satellite DNAs are among most rapidly evolving sequences in eukaryotic genome, usually differing significantly among closely related species. By inducing changes in heterochromatin and/or centromere, satellite DNAs are expected to drive population and species divergence. However, despite high evolutionary dynamics, divergence of satellite DNA profiles at the level of natural population which precedes and possibly triggers speciation process is not readily detected. Here, we characterize minor TCAST2 satellite DNA of the red flour beetle Tribolium castaneum and follow its dynamics among wild-type strains originating from diverse geographic locations. The investigation revealed presence of three distinct subfamilies of TCAST2 satellite DNA which differ in monomer size, genome organization, and subfamily specific mutations. Subfamilies Tcast2a and Tcast2b are tandemly arranged within pericentromeric heterochromatin whereas Tcast2c is preferentially dispersed within euchromatin of all chromosomes. Among strains, TCAST2 subfamilies are conserved in sequence but exhibit a significant content variability. This results in overrepresentation or almost complete absence of particular subfamily in some strains and enables discrimination between strains. It is proposed that homologous recombination, probably stimulated by environmental stress, is responsible for the emergence of TCAST2 satellite subfamilies, their copy number variation and dispersion within genome. The results represent the first evidence for the existence of population-specific satellite DNA profiles. Partial organization of TCAST2 satellite DNA in the form of single repeats dispersed within euchromatin additionally contributes to the genome divergence at the population level.

  12. Are there genetic trade-offs between immune and reproductive investments in Tribolium castaneum?

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    Hangartner, Sandra; Sbilordo, Sonja H; Michalczyk, Łukasz; Gage, Matthew J G; Martin, Oliver Y

    2013-10-01

    Parasites impose strong selection on hosts to defend themselves, which is expected to result in trade-offs with other fitness traits such as reproduction. Here we test for genetic trade-offs between reproductive traits and immunity using Tribolium castaneum lines that were subject to experimental evolution. The lines have been exposed to contrasting sexual selection intensities via different sex ratios (female-biased, equal and male-biased). After 56 generations, the lines have significantly diverged and those experiencing high sexual selection have evolved males who are superior competitors for reproductive success, and females who are more resistant to multiple mating. All selected lines were assessed for both an immune measurement (phenoloxidase (PO) activity) and host resistance to the microsporidian Nosema whitei after two generations of relaxed selection. In contrast to our expectations we did not find any evidence for a genetic trade-off between investment in reproduction and immunity. Both PO and Nosema resistance did not differ between lines, despite their divergences in reproductive investment due to variation in sexual selection and conflict. Nevertheless, overall we found that females had higher PO activities and in the Nosema free control treatment survived longer than males, suggesting that females generally invest more in PO and survival under control conditions than males. This result fits the Bateman's principle, which states that females gain fitness through increased immunity and longevity, while males gain fitness through increased mating success.

  13. Direct and indirect genetic effects in life-history traits of flour beetles (Tribolium castaneum).

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    Ellen, Esther D; Peeters, Katrijn; Verhoeven, Merel; Gols, Rieta; Harvey, Jeffrey A; Wade, Michael J; Dicke, Marcel; Bijma, Piter

    2016-01-01

    Indirect genetic effects (IGEs) are the basis of social interactions among conspecifics, and can affect genetic variation of nonsocial and social traits. We used flour beetles (Tribolium castaneum) of two phenotypically distinguishable populations to estimate genetic (co)variances and the effect of IGEs on three life-history traits: development time (DT), growth rate (GR), and pupal body mass (BM). We found that GR was strongly affected by social environment with IGEs accounting for 18% of the heritable variation. We also discovered a sex-specific social effect: male ratio in a group significantly affected both GR and BM; that is, beetles grew larger and faster in male-biased social environments. Such sex-specific IGEs have not previously been demonstrated in a nonsocial insect. Our results show that beetles that achieve a higher BM do so via a slower GR in response to social environment. Existing models of evolution in age-structured or stage-structured populations do not account for IGEs of social cohorts. It is likely that such IGEs have played a key role in the evolution of developmental plasticity shown by Tenebrionid larvae in response to density. Our results document an important source of genetic variation for GR, often overlooked in life-history theory.

  14. Constraints on the evolution of function-valued traits: a study of growth in Tribolium castaneum.

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    Irwin, K K; Carter, P A

    2013-12-01

    Growth trajectories often impact individual fitness. They are continuous by nature and so are amenable to analysis using a function-valued (FV) trait framework to reveal their underlying genetic architecture. Previous studies have found high levels of standing additive genetic (co)variance for growth trajectories despite the expectation that growth should be responding to frequent strong directional selection. In this study, the FV framework is used to estimate the additive genetic covariance function for growth trajectories in larval Tribolium castaneum to address questions about standing additive genetic (co)variance and possible evolutionary constraints on growth and to predict responses to four plausible selection regimes. Results show that additive genetic (co)variance is high at the early ages, but decreases towards later ages in the larval period. A selection gradient function of the same size and in the same direction of the first eigenfunction of the G-function should give the maximal response. However, evolutionary constraints may be acting to keep this maximal response from being realized, through either conflicting effects on survivability and fecundity of larger body size, few evolutionary directions having sufficient additive variance for a response, genetic trade-offs with other traits or physiological regulatory mechanisms. More light may be shed on these constraints through the development of more sophisticated statistical approaches and implementation of additional empirical studies to explicitly test for specific types of constraints.

  15. Gender- and stressor-specific microRNA expression in Tribolium castaneum.

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    Freitak, Dalial; Knorr, Eileen; Vogel, Heiko; Vilcinskas, Andreas

    2012-10-23

    MicroRNAs (miRNAs) are small non-coding RNAs mediating post-transcriptional regulation of gene expression in eukaryotes. Addressing their role in regulation of physiological adaptations to environmental stress in insects, we selected the red flour beetle Tribolium castaneum as a model. Beetles were fed with the bacterial entomopathogen Pseudomonas entomophila (to mimic natural infection), injected with peptidoglycan (experimental setting of strong immune responses) or subjected to either mild heat shock or starvation. Differential expression of selected immunity- and stress-related genes was quantified using real-time PCR, and expression and induction of 455 mature arthropod miRNAs were determined using proprietary microarrays. We found that Tribolium exhibits both gender- and stressor-specific adjustment of immune gene and miRNA expression. Strikingly, we discovered that the number of stressor-induced miRNAs in females is remarkably higher than in males. This observation could support the hypothesis called Bateman's principle in immunity that predicts gender-specific immune responses because females gain fitness through increased longevity, whereas males gain fitness by increasing mating rates. Our results suggest that Tribolium males and females display differential regulatory elements, both pre- and post-transcriptional, likely resulting from different investment strategies in life-history traits.

  16. Hormonal enhancement of insecticide efficacy in Tribolium castaneum: oxidative stress and metabolic aspects.

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    Plavšin, Ivana; Stašková, Tereza; Šerý, Michal; Smýkal, Vlastimil; Hackenberger, Branimir K; Kodrík, Dalibor

    2015-04-01

    Insect anti-stress responses, including those induced by insecticides, are controlled by adipokinetic hormones (AKHs). We examined the physiological consequences of Pyrap-AKH application on Tribolium castaneum adults (AKH-normal and AKH-deficient prepared by the RNAi technique) treated by two insecticides, pirimiphos-methyl and deltamethrin. Co-application of pirimiphos-methyl and/or deltamethrin with AKH significantly increased beetle mortality compared with application of the insecticides alone. This co-treatment was accompanied by substantial stimulation of general metabolism, as monitored by carbon dioxide production. Further, the insecticide treatment alone affected some basic markers of oxidative stress: it lowered total antioxidative capacity as well as the activity of superoxide dismutase in the beetle body; in addition, it enhanced the activity of catalase and glutathione-S-transferase. However, these discrepancies in oxidative stress markers were eliminated/reduced by co-application with Pyrap-AKH. We suggest that the elevation of metabolism, which is probably accompanied with faster turnover of toxins, might be responsible for the higher mortality that results after AKH and insecticide co-application. Changes in oxidative stress markers are probably not included in the mechanisms responsible for increased mortality.

  17. Molecular markers detect cryptic predation on coffee berry borer (Coleoptera: Curculionidae) by silvanid and laemophloeid flat bark beetles (Coleoptera: Silvanidae, Laemophloeidae) in coffee beans

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    The coffee berry borer, Hypothenemus hampei(Coleoptera: Curculionidae)(Ferrari), is a serious pest of coffee worldwide and has been recently introduced in Hawai’i, first detected in the state in 2010. Adult silvanid flat bark beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and adult laemoph...

  18. Comparative characterization of two intracellular Ca²⁺-release channels from the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Liu, Yaping; Li, Chengjun; Gao, Jingkun; Wang, Wenlong; Huang, Li; Guo, Xuezhu; Li, Bin; Wang, Jianjun

    2014-10-21

    Ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs) are members of a family of tetrameric intracellular Ca(2+)-release channels (CRCs). While it is well known in mammals that RyRs and IP3Rs modulate multiple physiological processes, the roles of these two CRCs in the development and physiology of insects remain poorly understood. In this study, we cloned and functionally characterized RyR and IP3R cDNAs (named TcRyR and TcIP3R) from the red flour beetle, Tribolium castaneum. The composite TcRyR gene contains an ORF of 15,285 bp encoding a protein of 5,094 amino acid residues. The TcIP3R contains an 8,175 bp ORF encoding a protein of 2,724 amino acids. Expression analysis of TcRyR and TcIP3R revealed significant differences in mRNA expression levels among T. castaneum during different developmental stages. When the transcript levels of TcRyR were suppressed by RNA interference (RNAi), an abnormal folding of the adult hind wings was observed, while the RNAi-mediated knockdown of TcIP3R resulted in defective larval-pupal and pupal-adult metamorphosis. These results suggested that TcRyR is required for muscle excitation-contraction (E-C) coupling in T. castaneum, and that calcium release via IP3R might play an important role in regulating ecdysone synthesis and release during molting and metamorphosis in insects.

  19. Glycogen and glucose metabolism are essential for early embryonic development of the red flour beetle Tribolium castaneum.

    Directory of Open Access Journals (Sweden)

    Amanda Fraga

    Full Text Available Control of energy metabolism is an essential process for life. In insects, egg formation (oogenesis and embryogenesis is dependent on stored molecules deposited by the mother or transcribed later by the zygote. In oviparous insects the egg becomes an isolated system after egg laying with all energy conversion taking place during embryogenesis. Previous studies in a few vector species showed a strong correlation of key morphogenetic events and changes in glucose metabolism. Here, we investigate glycogen and glucose metabolism in the red flour beetle Tribolium castaneum, an insect amenable to functional genomic studies. To examine the role of the key enzymes on glycogen and glucose regulation we cloned and analyzed the function of glycogen synthase kinase 3 (GSK-3 and hexokinase (HexA genes during T. castaneum embryogenesis. Expression analysis via in situ hybridization shows that both genes are expressed only in the embryonic tissue, suggesting that embryonic and extra-embryonic cells display different metabolic activities. dsRNA adult female injection (parental RNAi of both genes lead a reduction in egg laying and to embryonic lethality. Morphological analysis via DAPI stainings indicates that early development is impaired in Tc-GSK-3 and Tc-HexA1 RNAi embryos. Importantly, glycogen levels are upregulated after Tc-GSK-3 RNAi and glucose levels are upregulated after Tc-HexA1 RNAi, indicating that both genes control metabolism during embryogenesis and oogenesis, respectively. Altogether our results show that T. castaneum embryogenesis depends on the proper control of glucose and glycogen.

  20. Non-invasive long-term fluorescence live imaging of Tribolium castaneum embryos.

    Science.gov (United States)

    Strobl, Frederic; Stelzer, Ernst H K

    2014-06-01

    Insect development has contributed significantly to our understanding of metazoan development. However, most information has been obtained by analyzing a single species, the fruit fly Drosophila melanogaster. Embryonic development of the red flour beetle Tribolium castaneum differs fundamentally from that of Drosophila in aspects such as short-germ development, embryonic leg development, extensive extra-embryonic membrane formation and non-involuted head development. Although Tribolium has become the second most important insect model organism, previous live imaging attempts have addressed only specific questions and no long-term live imaging data of Tribolium embryogenesis have been available. By combining light sheet-based fluorescence microscopy with a novel mounting method, we achieved complete, continuous and non-invasive fluorescence live imaging of Tribolium embryogenesis at high spatiotemporal resolution. The embryos survived the 2-day or longer imaging process, developed into adults and produced fertile progeny. Our data document all morphogenetic processes from the rearrangement of the uniform blastoderm to the onset of regular muscular movement in the same embryo and in four orientations, contributing significantly to the understanding of Tribolium development. Furthermore, we created a comprehensive chronological table of Tribolium embryogenesis, integrating most previous work and providing a reference for future studies. Based on our observations, we provide evidence that serosa window closure and serosa opening, although deferred by more than 1 day, are linked. All our long-term imaging datasets are available as a resource for the community. Tribolium is only the second insect species, after Drosophila, for which non-invasive long-term fluorescence live imaging has been achieved.

  1. Visualizing late insect embryogenesis: extraembryonic and mesodermal enhancer trap expression in the beetle Tribolium castaneum.

    Directory of Open Access Journals (Sweden)

    Stefan Koelzer

    Full Text Available The beetle Tribolium castaneum has increasingly become a powerful model for comparative research on insect development. One recent resource is a collection of piggyBac transposon-based enhancer trap lines. Here, we provide a detailed analysis of three selected lines and demonstrate their value for investigations in the second half of embryogenesis, which has thus far lagged behind research on early stages. Two lines, G12424 and KT650, show enhanced green fluorescent protein (EGFP expression throughout the extraembryonic serosal tissue and in a few discrete embryonic domains. Intriguingly, both lines show for the first time a degree of regionalization within the mature serosa. However, their expression profiles illuminate distinct aspects of serosal biology: G12424 tracks the tissue's rapid maturation while KT650 expression likely reflects ongoing physiological processes. The third line, G04609, is stably expressed in mesodermal domains, including segmental muscles and the heart. Genomic mapping followed by in situ hybridization for genes near to the G04609 insertion site suggests that the transposon has trapped enhancer information for the Tribolium orthologue of midline (Tc-mid. Altogether, our analyses provide the first live imaging, long-term characterizations of enhancer traps from this collection. We show that EGFP expression is readily detected, including in heterozygote crosses that permit the simultaneous visualization of multiple tissue types. The tissue specificity provides live, endogenous marker gene expression at key developmental stages that are inaccessible for whole mount staining. Furthermore, the nonlocalized EGFP in these lines illuminates both the nucleus and cytoplasm, providing cellular resolution for morphogenesis research on processes such as dorsal closure and heart formation. In future work, identification of regulatory regions driving these enhancer traps will deepen our understanding of late developmental control

  2. Expression of an endoglucanase from Tribolium castaneum (TcEG1) in Saccharomyces cerevisiae.

    Science.gov (United States)

    Shirley, Derek; Oppert, Cris; Reynolds, Todd B; Miracle, Bethany; Oppert, Brenda; Klingeman, William E; Jurat-Fuentes, Juan Luis

    2014-10-01

    Insects are a largely unexploited resource in prospecting for novel cellulolytic enzymes to improve the production of ethanol fuel from lignocellulosic biomass. The cost of lignocellulosic ethanol production is expected to decrease by the combination of cellulose degradation (saccharification) and fermentation of the resulting glucose to ethanol in a single process, catalyzed by the yeast Saccharomyces cerevisiae transformed to express efficient cellulases. While S. cerevisiae is an established heterologous expression system, there are no available data on the functional expression of insect cellulolytic enzymes for this species. To address this knowledge gap, S. cerevisiae was transformed to express the full-length cDNA encoding an endoglucanase from the red flour beetle, Tribolium castaneum (TcEG1), and evaluated the activity of the transgenic product (rTcEG1). Expression of the TcEG1 cDNA in S. cerevisiae was under control of the strong glyceraldehyde-3 phosphate dehydrogenase promoter. Cultured transformed yeast secreted rTcEG1 protein as a functional β-1,4-endoglucanase, which allowed transformants to survive on selective media containing cellulose as the only available carbon source. Evaluation of substrate specificity for secreted rTcEG1 demonstrated endoglucanase activity, although some activity was also detected against complex cellulose substrates. Potentially relevant to uses in biofuel production rTcEG1 activity increased with pH conditions, with the highest activity detected at pH 12. Our results demonstrate the potential for functional production of an insect cellulase in S. cerevisiae and confirm the stability of rTcEG1 activity in strong alkaline environments.

  3. Signalling properties and pharmacology of a 5-HT7 -type serotonin receptor from Tribolium castaneum.

    Science.gov (United States)

    Vleugels, R; Lenaerts, C; Vanden Broeck, J; Verlinden, H

    2014-04-01

    In the last decade, genome sequence data and gene structure information on invertebrate receptors has been greatly expanded by large sequencing projects and cloning studies. This information is of great value for the identification of receptors; however, functional and pharmacological data are necessary for an accurate receptor classification and for practical applications. In insects, an important group of neurotransmitter and neurohormone receptors, for which ample sequence information is available but pharmacological information is missing, are the biogenic amine G protein-coupled receptors (GPCRs). In the present study, we investigated the sequence information, pharmacology and signalling properties of a 5-HT7 -type serotonin receptor from the red flour beetle, Tribolium castaneum (Trica5-HT7 ). The receptor encoding cDNA shows considerable sequence similarity with cognate 5-HT7 receptors and phylogenetic analysis also clusters the receptor within this 5-HT receptor group. Real-time reverse transcription PCR demonstrated high expression levels in the brain, indicating the possible importance of this receptor in neural processes. Trica5-HT7 was dose-dependently activated by 5-HT, which induced elevated intracellular cyclic AMP levels but had no effect on calcium signalling. The synthetic agonists, α-methyl 5-HT, 5-methoxytryptamine, 5-carboxamidotryptamine and 8-hydroxy-2-(dipropylamino)tetralin hydrobromide, showed a response, although with a much lower potency and efficacy than 5-HT. Ketanserin and methiothepin were the most potent antagonists. Both showed characteristics of competitive inhibition on Trica5-HT7 . The signalling pathway and pharmacological profile offer important information that will facilitate functional and comparative studies of 5-HT receptors in insects and other invertebrates. The pharmacology of invertebrate 5-HT receptors differs considerably from that of vertebrates. The present study may therefore contribute to establishing a more

  4. Visualizing late insect embryogenesis: extraembryonic and mesodermal enhancer trap expression in the beetle Tribolium castaneum.

    Science.gov (United States)

    Koelzer, Stefan; Kölsch, Yvonne; Panfilio, Kristen A

    2014-01-01

    The beetle Tribolium castaneum has increasingly become a powerful model for comparative research on insect development. One recent resource is a collection of piggyBac transposon-based enhancer trap lines. Here, we provide a detailed analysis of three selected lines and demonstrate their value for investigations in the second half of embryogenesis, which has thus far lagged behind research on early stages. Two lines, G12424 and KT650, show enhanced green fluorescent protein (EGFP) expression throughout the extraembryonic serosal tissue and in a few discrete embryonic domains. Intriguingly, both lines show for the first time a degree of regionalization within the mature serosa. However, their expression profiles illuminate distinct aspects of serosal biology: G12424 tracks the tissue's rapid maturation while KT650 expression likely reflects ongoing physiological processes. The third line, G04609, is stably expressed in mesodermal domains, including segmental muscles and the heart. Genomic mapping followed by in situ hybridization for genes near to the G04609 insertion site suggests that the transposon has trapped enhancer information for the Tribolium orthologue of midline (Tc-mid). Altogether, our analyses provide the first live imaging, long-term characterizations of enhancer traps from this collection. We show that EGFP expression is readily detected, including in heterozygote crosses that permit the simultaneous visualization of multiple tissue types. The tissue specificity provides live, endogenous marker gene expression at key developmental stages that are inaccessible for whole mount staining. Furthermore, the nonlocalized EGFP in these lines illuminates both the nucleus and cytoplasm, providing cellular resolution for morphogenesis research on processes such as dorsal closure and heart formation. In future work, identification of regulatory regions driving these enhancer traps will deepen our understanding of late developmental control, including in the

  5. Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum.

    Science.gov (United States)

    Gilles, Anna F; Schinko, Johannes B; Averof, Michalis

    2015-08-15

    Gene-editing techniques are revolutionizing the way we conduct genetics in many organisms. The CRISPR/Cas nuclease has emerged as a highly versatile, efficient and affordable tool for targeting chosen sites in the genome. Beyond its applications in established model organisms, CRISPR technology provides a platform for genetic intervention in a wide range of species, limited only by our ability to deliver it to cells and to select mutations efficiently. Here, we test the CRISPR technology in an emerging insect model and pest, the beetle Tribolium castaneum. We use simple assays to test CRISPR/Cas activity, we demonstrate efficient expression of guide RNAs and Cas9 from Tribolium U6 and hsp68 promoters and we test the efficiency of knockout and knock-in approaches in Tribolium. We find that 55-80% of injected individuals carry mutations (indels) generated by non-homologous end joining, including mosaic bi-allelic knockouts; 71-100% carry such mutations in their germ line and transmit them to the next generation. We show that CRISPR-mediated gene knockout of the Tribolium E-cadherin gene causes defects in dorsal closure, which is consistent with RNAi-induced phenotypes. Homology-directed knock-in of marker transgenes was observed in 14% of injected individuals and transmitted to the next generation by 6% of injected individuals. Previous work in Tribolium mapped a large number of transgene insertions associated with developmental phenotypes and enhancer traps. We present an efficient method for re-purposing these insertions, via CRISPR-mediated replacement of these transgenes by new constructs.

  6. Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera)

    NARCIS (Netherlands)

    Alexander, K.

    2003-01-01

    Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera) Data are presented on the distribution of selected species from two coleopteran families chosen to represent a random slice of the British fauna. The species have been chosen as exhibiting extremes of range chang

  7. A coffee berry borer (Coleoptera: Curculionidae: Scolytinae) bibliography

    Science.gov (United States)

    One hundred years ago, one of the most significant biological invasions of an agricultural insect pest in the Americas was initiated. Endemic to Africa, the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae) was accidentally introduced to Brazil in 1913 and years later invaded coffe...

  8. Noteworthy records of Hispines from Belize (Coleoptera: Chrysomelidae)

    OpenAIRE

    Naczi, Robert F. C.; Charles L. Staines

    2011-01-01

    Cephaloleia consanguinea Baly, Cephaloleia fulvolimbata Baly, Cephaloleia ruficollis Baly, Chalepus amabilis Baly, Chalepus brevicornis (Baly), Chalepus pici Descarpentries and Villiers, Microrhopala erebus (Newman), Octhispa bimaculata Uhmann, Octotoma championi Baly, Pseudispa tuberculata Staines, Sceloenopla erudita (Baly), Stenispa guatemalensis Uhmann, Sumitrosis gestroi (Weise), and Sumitrosis terminatus (Baly) (Coleoptera: Chrysomelidae: Cassidinae) are new country records of hispine c...

  9. Predation by Flat Bark Beetles (Coleoptera: Silvanidae and Laemophloeidae) on Coffee Berry Borer (Coleoptera: Curculionidae) in Hawaii coffee

    Science.gov (United States)

    Coffee berry borer(CBB), Hypothenemus hampei, is a serious pest of coffee worldwide and a new invasive pest in Hawaii. Adult flat bark beetles, mainly Leptophloeus sp.(75%) and Cathartus quadricollis(21%) (Coleoptera: Laemophloeidae and Silvanidae, respectively), were found feeding in CBB-infested c...

  10. “I don’t know a lot of rude words”: Herbst, Paretsky, and Grafton’s Struggle to Master the Tough Guy Voice

    Directory of Open Access Journals (Sweden)

    Laura Ng

    2013-09-01

    Full Text Available Abstract The proletarian literature of the 1930s and traditional 1930s era hard-boiled detective fiction are literary siblings. Both genres came of age in popular publications. Both genres deal with establishing order. Both genres use the “tough guy” voice. This voice was a no-nonsense powerful tool used to highlight the gritty realism of blue collar life. This style of writing quickly became a central component for both genres. Embedded in the term “tough guy voice” is masculine identity. This is the voice for the active underdog male hero. Writing in genres for women is an on-going challenge given the expectation of gender implied in the narrative voice. For the contemporary feminist hard-boiled detective and the 1930’s women proletarian protagonist to be taken seriously in their respective tough guy genres, they must show the same verbal acumen as the male characters who built the genres. At the core of each genre, the expectation that tough guys remain tough guys is an issue that stretches beyond the time frame of 1930s and 1940s to contemporary iterations of the genres. The gendered transition from male protagonist to female protagonist requires authors like Josephine Herbst, Sara Paretsky, and Sue Grafton to engage in a series of dynamic manipulations verbal and textual manipulations. Both sets of authors created protagonists that faced the threat of being dismissed due to their gender, traditional family roles, and conflicts with authority. The authors dealt with these threats using very similar strategies, to varying levels of success. Paretsky and Grafton’s heroines win verbal battles by showing mastery of the voice and the other voices in the texts.  At the core of this struggle is the protagonists’ ability to maintain the power of professing.

  11. Het voorkomen van de glanskevers van het genus Caprophilus in Nederland (Coleoptera: Nitidulidae)

    NARCIS (Netherlands)

    Oude, de J.

    2005-01-01

    The occurrence of nitidulid beetles of the genus Carpophilus in the Netherlands (Coleoptera: Nitidulidae) Some species of the genus Carpophilus (Coleoptera: Nitidulidae) may act as pests of stored products like cocoabeans, peanuts, dried fruits like figs and dates, imported from tropical or subtropi

  12. Proteome response of Tribolium castaneum larvae to Bacillus thuringiensis toxin producing strains.

    Directory of Open Access Journals (Sweden)

    Estefanía Contreras

    Full Text Available Susceptibility of Tribolium castaneum (Tc larvae was determined against spore-crystal mixtures of five coleopteran specific and one lepidopteran specific Bacillus thuringiensis Cry toxin producing strains and those containing the structurally unrelated Cry3Ba and Cry23Aa/Cry37Aa proteins were found toxic (LC(50 values 13.53 and 6.30 µg spore-crystal mixture/µL flour disc, respectively. Using iTRAQ combined with LC-MS/MS allowed the discovery of seven novel differentially expressed proteins in early response of Tc larvae to the two active spore-crystal mixtures. Proteins showing a statistically significant change in treated larvae compared to non-intoxicated larvae fell into two major categories; up-regulated proteins were involved in host defense (odorant binding protein C12, apolipophorin-III and chemosensory protein 18 and down-regulated proteins were linked to metabolic pathways affecting larval metabolism and development (pyruvate dehydrogenase Eα subunit, cuticular protein, ribosomal protein L13a and apolipoprotein LI-II. Among increased proteins, Odorant binding protein C12 showed the highest change, 4-fold increase in both toxin treatments. The protein displayed amino acid sequence and structural homology to Tenebrio molitor 12 kDa hemolymph protein b precursor, a non-olfactory odorant binding protein. Analysis of mRNA expression and mortality assays in Odorant binding protein C12 silenced larvae were consistent with a general immune defense function of non-olfactory odorant binding proteins. Regarding down-regulated proteins, at the transcriptional level, pyruvate dehydrogenase and cuticular genes were decreased in Tc larvae exposed to the Cry3Ba producing strain compared to the Cry23Aa/Cry37Aa producing strain, which may contribute to the developmental arrest that we observed with larvae fed the Cry3Ba producing strain. Results demonstrated a distinct host transcriptional regulation depending upon the Cry toxin treatment. Knowledge

  13. Progresses in research on the functional genomics on Tribolium castaneum%赤拟谷盗功能基因组学研究进展

    Institute of Scientific and Technical Information of China (English)

    李承军; 王艳允; 刘幸; 桑明; 李斌

    2011-01-01

    赤拟谷盗Tribolium castaneum是一种重要的模式生物,在遗传、发育、生化与免疫等研究领域均取得了重要的研究进展.同时它也是一种危害极大的鞘翅目类储粮害虫,在世界各地都有分布,每年给储藏物造成了数十亿美元的经济损失.其全基因组测序的完成、遗传操作体系的构建及系统RNAi方法的应用都极大地促进了其功能基因组学的研究.本文综述了近年来赤拟谷盗基因组计划及功能基因组学的研究进展,拟为赤拟谷盗的生物学研究和防治奠定基础.%Tribolium castaneum is a powerful model organism for research in such fields as insect genetics, biology development, biochemistry and immunity, and rapid and useful progress has been made in these fields in recent years. T. Castaneum is also an important coleopteran pest of stored agricultural products. This globally distributed pest causes billions of dollars of damage to such stored products each year. Recently, the sequencing of the entire genome of T. Castaneum, the construction of their genetics operation systems, and the utilization of the systemic RNA interference, have greatly facilitated and accelerated research on their functional genomics and the related fields. In order to facilitate research on both the fundamental biology and control of T. Castaneum, we here review progresses and achievements in research on T. Castaneum genome projects and functional genomics over the past few years.

  14. Family-Group Names In Coleoptera (Insecta

    Directory of Open Access Journals (Sweden)

    Patrice Bouchard

    2011-04-01

    Full Text Available We synthesize data on all known extant and fossil Coleoptera family-group names for the first time. A catalogue of 4887 family-group names (124 fossil, 4763 extant based on 4707 distinct genera in Coleoptera is given. A total of 4492 names are available, 183 of which are permanently invalid because they are based on a preoccupied or a suppressed type genus. Names are listed in a classification framework. We recognize as valid 24 superfamilies, 211 families, 541 subfamilies, 1663 tribes and 740 subtribes. For each name, the original spelling, author, year of publication, page number, correct stem and type genus are included. The original spelling and availability of each name were checked from primary literature. A list of necessary changes due to Priority and Homonymy problems, and actions taken, is given. Current usage of names was conserved, whenever possible, to promote stability of the classification. New synonymies (family-group names followed by genus-group names: Agronomina Gistel, 1848 syn. n. of Amarina Zimmermann, 1832 (Carabidae, Hylepnigalioini Gistel, 1856 syn. n. of Melandryini Leach, 1815 (Melandryidae, Polycystophoridae Gistel, 1856 syn. n. of Malachiinae Fleming, 1821 (Melyridae, Sclerasteinae Gistel, 1856 syn. n. of Ptilininae Shuckard, 1839 (Ptinidae, Phloeonomini Ádám, 2001 syn. n. of Omaliini MacLeay, 1825 (Staphylinidae, Sepedophilini Ádám, 2001 syn. n. of Tachyporini MacLeay, 1825 (Staphylinidae, Phibalini Gistel, 1856 syn. n. of Cteniopodini Solier, 1835 (Tenebrionidae; Agronoma Gistel 1848 (type species Carabus familiaris Duftschmid, 1812, designated herein syn. n. of Amara Bonelli, 1810 (Carabidae, Hylepnigalio Gistel, 1856 (type species Chrysomela caraboides Linnaeus, 1760, by monotypy syn. n. of Melandrya Fabricius, 1801 (Melandryidae, Polycystophorus Gistel, 1856 (type species Cantharis aeneus Linnaeus, 1758, designated herein syn. n. of Malachius Fabricius, 1775 (Melyridae, Sclerastes Gistel, 1856 (type species

  15. Loss of function of the yellow-e gene causes dehydration-induced mortality of adult Tribolium castaneum.

    Science.gov (United States)

    Noh, Mi Young; Kramer, Karl J; Muthukrishnan, Subbaratnam; Beeman, Richard W; Kanost, Michael R; Arakane, Yasuyuki

    2015-03-15

    Yellow protein (dopachrome conversion enzyme, DCE) is involved in the melanin biosynthetic pathway that significantly accelerates pigmentation reactions in insects. Recent studies have suggested that the insect yellow genes represent a rapidly evolving gene family generating functionally diverse paralogs, but the exact physiological functions of several yellow genes are still not understood. To study the function(s) of one of the yellow genes, yellow-e (TcY-e), in the red flour beetle, Tribolium castaneum, we performed real-time PCR to analyze its developmental and tissue-specific expression, and utilized immunohistochemistry to identify the localization of the TcY-e protein in adult cuticle. Injection of double-stranded RNA for TcY-e (dsTcY-e) into late instar larvae had no effect on larval-pupal molting or pupal development. The pupal cuticle, including that lining the setae, gin traps and urogomphi, underwent normal tanning. Adult cuticle tanning including that of the head, mandibles and legs viewed through the translucent pupal cuticle was initiated on schedule (pupal days 4-5), indicating that TcY-e is not required for pupal or pharate adult cuticle pigmentation in T. castaneum. The subsequent pupal-adult molt, however, was adversely affected. Although pupal cuticle apolysis and slippage were evident, some of the adults (~25%) were unable to shed their exuvium and died entrapped in their pupal cuticle. In addition, the resulting adults rapidly became highly desiccated. Interestingly, both the failure of the pupal-adult molt and desiccation-induced mortality were prevented by maintaining the dsTcY-e-treated insects at 100% relative humidity (rh). However, when the high humidity-rescued adults were removed from 100% rh and transferred to 50% rh, they rapidly dehydrated and died, whereas untreated beetles thrived throughout development at 50% rh. We also observed that the body color of the high humidity-rescued dsTcY-e-adults was slightly darker than that of

  16. Knickkopf and retroactive proteins are required for formation of laminar serosal procuticle during embryonic development of Tribolium castaneum.

    Science.gov (United States)

    Chaudhari, Sujata S; Noh, Mi Young; Moussian, Bernard; Specht, Charles A; Kramer, Karl J; Beeman, Richard W; Arakane, Yasuyuki; Muthukrishnan, Subbaratnam

    2015-05-01

    Chitin, a homopolymer of β-1-4-linked N-acetylglucosamine synthesized by chitin synthase A (Chs-A), is organized in the procuticle of the postembryonic cuticle or exoskeleton, which is composed of laminae stacked parallel to the cell surface to give stability and integrity to the underlying insect epidermal and other tissues. Our previous work has revealed an important role for two proteins from Tribolium castaneum named Knickkopf (TcKnk) and Retroactive (TcRtv) in postembryonic cuticular chitin maintenance. TcKnk and TcRtv were shown to be required for protection and organization of newly synthesized procuticular chitin. To study the functions of TcKnk and TcRtv in serosal and larval cuticles produced during embryogenesis in T. castaneum, dsRNAs specific for these two genes were injected into two week-old adult females. The effects of dsRNA treatment on ovarial integrity, oviposition, egg hatching and adult survival were determined. Insects treated with dsRNA for chitin synthase-A (TcChs-A) and tryptophan oxygenase (TcVer) were used as positive and negative controls for these experiments, respectively. Like TcChs-A RNAi, injection of dsRNA for TcKnk or TcRtv into adult females exhibited no adult lethality and oviposition was normal. However, a vast majority of the embryos did not hatch. The remaining (∼10%) of the embryos hatched into first instar larvae that died without molting to the second instar. Chitin content analysis following TcKnk and TcRtv parental RNAi revealed approximately 50% reduction in chitin content of eggs in comparison with control TcVer RNAi, whereas TcChs-A dsRNA-treatment led to >90% loss of chitin. Furthermore, transmission electron microscopic (TEM) analysis of serosal cuticle from TcChs-A, TcKnk and TcRtv dsRNA-treated insects revealed a complete absence of laminar organization of serosal (and larval) procuticle in comparison with TcVer dsRNA-treated controls, which exhibited normal laminar organization of procuticular chitin. The

  17. A checklist of stag beetles (Coleoptera: Scarabaeoidea: Lucanidae) from Iran.

    Science.gov (United States)

    Bartolozzi, Luca; Ghahari, Hassan; Sprecher-Uebersax, Eva; Zilioli, Michele

    2014-11-26

    An updated checklist of the Lucanidae (Coleoptera) from Iran is given. New locality records are listed and some dubious distributional records are discussed. Dorcus vavrai Nonfried, 1905 is placed in synonymy with Dorcus peyronis Reiche and Saulcy, 1856 (new synonymy) The female of Lucanus xerxes Král, 2004 is described. A key for the identification of the Iranian stag beetle species is also provided and all the species are figured.

  18. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, Jessica; Verheggen, François; Lognay, Georges; Haubruge, Eric

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and...

  19. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, J.; Verheggen, F.; Lognay, G.; Haubruge, E.

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and ...

  20. RNAi-mediated knockdown of the voltage gated sodium ion channel TcNav causes mortality in Tribolium castaneum

    Science.gov (United States)

    Abd El Halim, Hesham M.; Alshukri, Baida M. H.; Ahmad, Munawar S.; Nakasu, Erich Y. T.; Awwad, Mohammed H.; Salama, Elham M.; Gatehouse, Angharad M. R.; Edwards, Martin G.

    2016-01-01

    The voltage-gated sodium ion channel (VGSC) belongs to the largest superfamily of ion channels. Since VGSCs play key roles in physiological processes they are major targets for effective insecticides. RNA interference (RNAi) is widely used to analyse gene function, but recently, it has shown potential to contribute to novel strategies for selectively controlling agricultural insect pests. The current study evaluates the delivery of dsRNA targeted to the sodium ion channel paralytic A (TcNav) gene in Tribolium castaneum as a viable means of controlling this insect pest. Delivery of TcNav dsRNA caused severe developmental arrest with larval mortalities up to 73% post injection of dsRNA. Injected larvae showed significant (p insect control. PMID:27411529

  1. Nocturnal Migration of Coleoptera: Carabidae in North China

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Vertical-looking radar (VLR) has allowed long-term automatic monitoring of the altitudinal and temporal dynamics of high-flying insect populations. To investigate whether ground beetle, insect of Coleoptera, was capable of migrating and its migration pattern by taking advantages of capability of the VLR for long-term real-time automatic monitoring, the migration of Coleoptera ground beetle was investigated by setting up radar observation points, making long-term observation using the VLR and related supplementary equipment, and analyzing low altitude air current and large area circulating current in combination with the meteorological data. Information obtained in 2005 and 2006 showed that the seasonal activities of ground beetles traps of trap lamps were mainly from late June to late August, peak period was mainly in August, seasonal traps of high-altitude lamps and ground lamps were featured by sudden increase and sudden decrease; in peak period, the height of radar echo point could be as high as 600 m, while it was mainly below the height of 450 m; night activities mainly occurred from 20:00 to 22:00, in very few nights, radar echo could last until about 04:00, changes in numbers of ground beetles within the searchlights were consistent with radar echo intensity; ground beetle images were successfully trapped in the sweep nets carried by captive balloons at the height of 200 m. Some species of Carabidaes had some degrees of migration, thus providing the foundation for investigating the migration of Coleoptera insects.

  2. Zum gegenwärtigen Stande des Problems der spezifischen Wärme Arnold Eucken, ed., Die Theorie der Strahlung und der Quanten. Verhandlungen auf einer von E. Solvay einberufenen Zusammenkunft (30. Oktober bis 3. November 1911), mit einem Anhange über die Entwicklung der Quantentheorie vom Herbst 1911 bis Sommer 1913. Halle a.S.: Knapp, 1914., 3 Nov 1911

    CERN Document Server

    Einstein, A

    1911-01-01

    Zum gegenwärtigen Stande des Problems der spezifischen Wärme Arnold Eucken, ed., Die Theorie der Strahlung und der Quanten. Verhandlungen auf einer von E. Solvay einberufenen Zusammenkunft (30. Oktober bis 3. November 1911), mit einem Anhange über die Entwicklung der Quantentheorie vom Herbst 1911 bis Sommer 1913. Halle a.S.: Knapp, 1914., 3 Nov 1911

  3. Zum gegenwärtigen Stande des Problems der spezifischen Wärme Arnold Eucken, ed., Die Theorie der Strahlung und der Quanten. Verhandlungen auf einer von E. Solvay einberufenen Zusammenkunft (30. Oktober bis 3. November 1911), mit einem Anhange über die Entwicklung der Quantentheorie vom Herbst 1911 bis Sommer 1913. Halle a.S.: Knapp, 1914., 3 Nov 1911

    CERN Document Server

    Einstein, A

    2003-01-01

    Zum gegenwärtigen Stande des Problems der spezifischen Wärme Arnold Eucken, ed., Die Theorie der Strahlung und der Quanten. Verhandlungen auf einer von E. Solvay einberufenen Zusammenkunft (30. Oktober bis 3. November 1911), mit einem Anhange über die Entwicklung der Quantentheorie vom Herbst 1911 bis Sommer 1913. Halle a.S.: Knapp, 1914., 3 Nov 1911

  4. Transcription profiling of immune genes during parasite infection in susceptible and resistant strains of the flour beetles (Tribolium castaneum).

    Science.gov (United States)

    Zhong, Daibin; Wang, Mei-Hui; Pai, Aditi; Yan, Guiyun

    2013-05-01

    The flour beetle, Tribolium castaneum, is an intermediate host for the tapeworm Hymenolepis diminuta and has become an important genetic model to explore immune responses to parasite infection in insect hosts. The present study examined the immune responses to tapeworm infection in resistant (TIW1) and susceptible (cSM) strains of the red flour beetle, T. castaneum, using real-time quantitative reverse transcription PCR on 29 immunity-related genes that exhibit antimicrobial properties. Thirteen of the 29 genes showed constitutive differences in expression between the two strains. Fourteen to fifteen of the 29 genes exhibited significant differences in transcription levels when beetles were challenged with tapeworm parasite in the resistant and susceptible strains. Nine genes (GNBP3, cSPH2, lysozyme4, defensin1, PGRP-SA, defensin2, coleoptericin1, attacin2 and serpin29) in cSM and 13 genes (lysozyme2, proPO1, GNBP3, cSPH2, lysozyme4, defensin1, PGRP-SA, defensin2, coleoptericin1, attacin2, proPO2/3, PGRP-LE and PGRP-SB) in TIW1 were up-regulated by infections or showed parasite infection-induced expression. Seven genes (attacin2, coleoptericin1, defensin1, defensin2, lysozyme2, PGRP-SA and PGRP-SB) were more than 10 folds higher in the resistant TIW1 strain than in the susceptible cSM strain after exposure to tapeworm parasites. This study demonstrated the effects of genetic background, the transcription profile to parasite infection, and identified the immunity-related genes that were significantly regulated by the infection of tapeworms in Tribolium beetles.

  5. A Defensin from the Model Beetle Tribolium castaneum Acts Synergistically with Telavancin and Daptomycin against Multidrug Resistant Staphylococcus aureus.

    Directory of Open Access Journals (Sweden)

    Rajmohan Rajamuthiah

    Full Text Available The red flour beetle Tribolium castaneum is a common insect pest and has been established as a model beetle to study insect development and immunity. This study demonstrates that defensin 1 from T. castaneum displays in vitro and in vivo antimicrobial activity against drug resistant Staphylococcus aureus strains. The minimum inhibitory concentration (MIC of defensin 1 against 11 reference and clinical staphylococcal isolates was between 16-64 μg/ml. The putative mode of action of the defensin peptide is disruption of the bacterial cell membrane. The antibacterial activity of defensin 1 was attenuated by salt concentrations of 1.56 mM and 25 mM for NaCl and CaCl2 respectively. Treatment of defensin 1 with the reducing agent dithiothreitol (DTT at concentrations 1.56 to 3.13 mM abolished the antimicrobial activity of the peptide. In the presence of subinhibitory concentrations of antibiotics that also target the bacterial cell envelope such as telavancin and daptomycin, the MIC of the peptide was as low as 1 μg/ml. Moreover, when tested against an S. aureus strain that was defective in D-alanylation of the cell wall, the MIC of the peptide was 0.5 μg/ml. Defensin 1 exhibited no toxicity against human erythrocytes even at 400 μg/ml. The in vivo activity of the peptide was validated in a Caenorhabditis elegans-MRSA liquid infection assay. These results suggest that defensin 1 behaves similarly to other cationic AMPs in its mode of action against S. aureus and that the activity of the peptide can be enhanced in combination with other antibiotics with similar modes of action or with compounds that have the ability to decrease D-alanylation of the bacterial cell wall.

  6. A Defensin from the Model Beetle Tribolium castaneum Acts Synergistically with Telavancin and Daptomycin against Multidrug Resistant Staphylococcus aureus.

    Science.gov (United States)

    Rajamuthiah, Rajmohan; Jayamani, Elamparithi; Conery, Annie L; Fuchs, Beth Burgwyn; Kim, Wooseong; Johnston, Tatiana; Vilcinskas, Andreas; Ausubel, Frederick M; Mylonakis, Eleftherios

    2015-01-01

    The red flour beetle Tribolium castaneum is a common insect pest and has been established as a model beetle to study insect development and immunity. This study demonstrates that defensin 1 from T. castaneum displays in vitro and in vivo antimicrobial activity against drug resistant Staphylococcus aureus strains. The minimum inhibitory concentration (MIC) of defensin 1 against 11 reference and clinical staphylococcal isolates was between 16-64 μg/ml. The putative mode of action of the defensin peptide is disruption of the bacterial cell membrane. The antibacterial activity of defensin 1 was attenuated by salt concentrations of 1.56 mM and 25 mM for NaCl and CaCl2 respectively. Treatment of defensin 1 with the reducing agent dithiothreitol (DTT) at concentrations 1.56 to 3.13 mM abolished the antimicrobial activity of the peptide. In the presence of subinhibitory concentrations of antibiotics that also target the bacterial cell envelope such as telavancin and daptomycin, the MIC of the peptide was as low as 1 μg/ml. Moreover, when tested against an S. aureus strain that was defective in D-alanylation of the cell wall, the MIC of the peptide was 0.5 μg/ml. Defensin 1 exhibited no toxicity against human erythrocytes even at 400 μg/ml. The in vivo activity of the peptide was validated in a Caenorhabditis elegans-MRSA liquid infection assay. These results suggest that defensin 1 behaves similarly to other cationic AMPs in its mode of action against S. aureus and that the activity of the peptide can be enhanced in combination with other antibiotics with similar modes of action or with compounds that have the ability to decrease D-alanylation of the bacterial cell wall.

  7. Estados inmaduros de Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini Immature stages of Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini

    Directory of Open Access Journals (Sweden)

    Jhon César Neita-Moreno

    2008-12-01

    Full Text Available Se describen y se ilustran por primera vez la larva de tercer estadio y la pupa de Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini. Se aportan datos sobre la biología de la especie y su distribución en Colombia. Se proporciona una clave para la identificación de las larvas de tercer estadio conocidas de las especies del género Ancognatha Erichson.The third instar larva and pupa of Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini are described and illustrated for the first time. New data on larval biology and distribution of the species in Colombia are included. A key to the known third stage larvae of Ancognatha Erichson is provided.

  8. Laboratory Bioassay of Iranian Isolates of Entomopathogenic Fungus Metarhizium anisopliae (Metsch. Sorokin (Ascomycota: Hypocreales against two Species of Storage Pest

    Directory of Open Access Journals (Sweden)

    Hamid Sakenin Chelav

    2013-03-01

    Full Text Available The susceptibility of adults of Tribolium castaneum Herbst (Coleoptera: Tenebrionidae and Oryzaephilus surinamensis L. (Coleoptera: Silvanidae to three Iranian Isolates of the entomopathogenic fungi Metarhizium anisopliae (Metsch. Sorokin was evaluated through bioassays with direct immersion under laboratory conditions. For each isolates, five aqueous suspensions were prepared in a logarithmic series in Tween 80 (0.05% v/v. Results showed that adult of both species were susceptible to all isolates of M. anisopliae. For all three isolates, mortality percentage of the two species increased with increasing conidial concentration and significant difference was observed between concentrations. The corrected cumulative mortality of adult of O. surinamensis 10 days after immersion ranged from 12.38 to 85.84%, 18.6 to 62.83% and 10.63 to 77.87% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. These amounts for T. castaneum varied from 31.07 to 74.78%, 26.02 to 75.61% and 23.33 to 89.99% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. The parameters of probit analysis demonstrated non-overlap of 95% confidence limits of LC50 and LC95 and significant difference was observed among three isolates tested against each insect. The lowest and the highest LC50 and LC95 values were observed in the isolates DEMI001 for O. surinamensis (3/1×105 and 1/5×108 and IRAN 715C for T. castaneum (6.2×108 and 6.9×1014, respectively. This observation highlights the importance and need of screening for more virulent isolates against storage pests for use in the management of these pests.

  9. A new species of the genus Falsoibidion Pic (Coleoptera, Cerambycidae) from Korea

    OpenAIRE

    Lee, Seunghyun; Lee, Seunghwan

    2016-01-01

    A new species of the genus Falsoibidion Pic, 1922 (Coleoptera, Cerambycidae, Cerambycinae, Callidiopini) from Korea is described. Habitus and genitalia of male and female of the new species are illustrated.

  10. A new species of the genus Falsoibidion Pic (Coleoptera, Cerambycidae) from Korea

    Science.gov (United States)

    Lee, Seunghyun; Lee, Seunghwan

    2016-01-01

    Abstract A new species of the genus Falsoibidion Pic, 1922 (Coleoptera, Cerambycidae, Cerambycinae, Callidiopini) from Korea is described. Habitus and genitalia of male and female of the new species are illustrated. PMID:27563272

  11. Parasitylenchus sp. (Tylenchomorpha: Allantonematidae) parasitizing field populations of Harmonia axyridis (Coleoptera: Coccinellidae)

    DEFF Research Database (Denmark)

    Harding, Susanne; Poinar, George O. Jr.; Dimitrova, Desislava V.;

    2011-01-01

    Adults of the invasive harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae), were found to be parasitized by nematodes (Tylenchomorpha: Allantonematidae) in Denmark. The nematodes were identified as Parasitylenchus sp. Major morphological characters of the nematodes did not differ...

  12. Significantly higher Carabid beetle (Coleoptera: Carabidae) catch in conventionally than in organically managed Christmas tree plantations

    DEFF Research Database (Denmark)

    Bagge, Søren; Lund, Malthe; Rønn, Regin;

    2012-01-01

    trapped carabid beetles (Coleoptera, Carabidae) varied between conventionally and organically managed Caucasian Fir (Abies nordmanniana (Stev.)) plantations, in northern Zealand, Denmark. We recorded significantly higher numbers of carabid beetle specimens and species at conventionally than at organically...

  13. Apostasimerini (Coleoptera: Curculionidae: Baridinae). Rectification of authorship, year of publication, rank, and taxa included

    Science.gov (United States)

    The following nomenclatural changes are proposed in the Coleoptera, Curculionidae: the author of Apostasimerini is Schoenherr (1844), not Lacordaire (1866); Madopterini Lacordaire, 1866 is demoted to subtribe of Apostasimerini; Erirhinus mourei Bondar, 1943 is a new synonym of Apostasimerus serriros...

  14. First record of Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae in Brazil

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2015-03-01

    Full Text Available Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae is recorded for the first time in Brazil (Bahia. It was originally described from Europe and is currently widely distributed in that continent and Asia.

  15. Primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae and Disteniidae) of the Smithsonian Institution

    Science.gov (United States)

    The primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae, Disteniidae) of the National Museum of Natural History (Smithsonian Institution) are catalogued and figured, current through 2012 (but also including some 2013 holotypes). Data on the original combination, current combina...

  16. Methods for assessing infestations of sunflower stem weevil (Coleoptera: Curculionidae) in sunflower stems

    Science.gov (United States)

    The sunflower stem weevil, Cylindrocopturus adspersus LeConte (Coleoptera: Curculionidae), reduces sunflower, Helianthus annuus L. (Asteraceae), yields by spreading pathogens, damaging vascular tissues, and promoting lodging of sunflower plants. To assess weevil populations for host plant resistanc...

  17. A new species of Scymnobius Casey (Coleoptera, Coccinellidae, Scymnini from Pernambuco, Brazil

    Directory of Open Access Journals (Sweden)

    José Adriano Giorgi

    2014-12-01

    Full Text Available A new species of Scymnobius Casey (Coleoptera, Coccinellidae, Scymnini from Pernambuco, Brazil. Scymnobius pernambucensis sp. nov. from Pernambuco, Brazil, is described and illustrated. This is the third species of this genus recorded from Brazil.

  18. Revision of the genus Altitatiayus Weinreich(Coleoptera, Lucanidae, Lucaninae

    Directory of Open Access Journals (Sweden)

    Paschoal C. Grossi

    2010-01-01

    Full Text Available Revision of the genus Altitatiayus Weinreich (Coleoptera, Lucanidae, Lucaninae. The South American genus Altitatiayus Weinreich is revised and now includes six species, A. rotundatus (Boileau, A. ruficollis (Lüderwaldt, A. godinhorum (Bomans & Arnaud, A. dulceae (Bomans & Arnaud, A. trifurcatus (Grossi & Racca-Filho and A. koikei sp. nov. (Minas Gerais, Brazil. All species are described and illustrated. For the first time male and female genitalia are illustrated for five species and observations on the behavior of two species are included.

  19. Papel dos besouros (Insecta, Coleoptera) na Entomologia Forense

    OpenAIRE

    Wellington Emanuel dos Santos

    2014-01-01

    Esse trabalho apresenta uma revisão do papel que os besouros (Insecta, Coleoptera) desempenham na Entomologia Forense. Discussões sobre ocorrência em cadáveres humanos e carcaças animais, estimativas de Intervalo Pós-Morte (IPM), estudos realizados no Brasil e em outros países, principais famílias de importância forense e aspectos biológicos, ecológicos e biogeográficos das espécies são apresentadas.

  20. Fossil history of Mesozoic weevils (Coleoptera:Curculionoidea)

    Institute of Scientific and Technical Information of China (English)

    Andrei A.Legalov

    2012-01-01

    The first synopsis of Mesozoic weevils (Curculionoidea: Coleoptera) is presented.Changes of family,genera and species abundance during the Mesozoic revealed three distributional patterns.The Jurassic (Karatau) fauna was dominated by the Nemonychidae.During the Early Cretaceous (beginning at the Jurassic/Cretaceous border),the Ithyceridae was the prevalent group with a significant role played by the Nemonychidae.In the Late Cretaceous (Cenomanian and Turonian),the major groups were the Curculionidae and Brentidae.Obviously,the change of weevil fauna during this period was due to the expansion of the angiosperms,which provided multiple niches in their vegetative and reproductive organs for weevil development.

  1. RNAi-mediated knockdown of the voltage gated sodium ion channel TcNav causes mortality in Tribolium castaneum

    Science.gov (United States)

    Abd El Halim, Hesham M.; Alshukri, Baida M. H.; Ahmad, Munawar S.; Nakasu, Erich Y. T.; Awwad, Mohammed H.; Salama, Elham M.; Gatehouse, Angharad M. R.; Edwards, Martin G.

    2016-01-01

    The voltage-gated sodium ion channel (VGSC) belongs to the largest superfamily of ion channels. Since VGSCs play key roles in physiological processes they are major targets for effective insecticides. RNA interference (RNAi) is widely used to analyse gene function, but recently, it has shown potential to contribute to novel strategies for selectively controlling agricultural insect pests. The current study evaluates the delivery of dsRNA targeted to the sodium ion channel paralytic A (TcNav) gene in Tribolium castaneum as a viable means of controlling this insect pest. Delivery of TcNav dsRNA caused severe developmental arrest with larval mortalities up to 73% post injection of dsRNA. Injected larvae showed significant (p < 0.05) knockdown in gene expression between 30–60%. Expression was also significantly (p < 0.05) reduced in pupae following injection causing 30% and 42% knockdown for early and late pupal stages, respectively. Oral delivery of dsRNA caused dose-dependant mortalities of between 19 and 51.34%; this was accompanied by significant (p < 0.05) knockdown in gene expression following 3 days of continuous feeding. The majority of larvae injected with, or fed, dsRNA died during the final larval stage prior to pupation. This work provides evidence of a viable RNAi-based strategy for insect control. PMID:27411529

  2. Experimental removal of sexual selection leads to decreased investment in an immune component in female Tribolium castaneum.

    Science.gov (United States)

    Hangartner, Sandra; Michalczyk, Łukasz; Gage, Matthew J G; Martin, Oliver Y

    2015-07-01

    Because of divergent selection acting on males and females arising from different life-history strategies, polyandry can be expected to promote sexual dimorphism of investment into immune function. In previous work we have established the existence of such divergence within populations where males and females are exposed to varying degrees of polyandry. We here test whether the removal of sexual selection via enforced monogamy generates males and females that have similar levels of investment in immune function. To test this prediction experimentally, we measured differences between the sexes in a key immune measurement (phenoloxidase (PO) activity) and resistance to the microsporidian Paranosema whitei in Tribolium castaneum lines that evolved under monogamous (sexual selection absent) vs polyandrous (sexual selection present) mating systems. At generation 49, all selected lines were simultaneously assessed for PO activity and resistance to their natural parasite P. whitei after two generations of relaxed selection. We found that the polyandrous regime was associated with a clear dimorphism in immune function: females had significantly higher PO activities than males in these lines. In contrast, there was no such difference between the sexes in the lines evolving under the monogamous regime. Survival in the infection experiment did not differ between mating systems or sexes. Removing sexual selection via enforced monogamy thus seems to erase intersexual differences in immunity investment. We suggest that higher PO activities in females that have evolved under sexual selection might be driven by the increased risk of infections and/or injuries associated with exposure to multiple males. PMID:25958137

  3. 3D standard brain of the red flour beetle Tribolium castaneum: a tool to study metamorphic development and adult plasticity

    Directory of Open Access Journals (Sweden)

    David Dreyer

    2010-03-01

    Full Text Available The red flour beetle Tribolium castaneum is emerging as a further standard insect model beside Drosophila. Its genome is fully sequenced and it is susceptible for genetic manipulations including RNA-interference. We use this beetle to study adult brain development and plasticity primarily with respect to the olfactory system. In the current study, we provide 3D standard brain atlases of freshly eclosed adult female and male beetles (A0. The atlases include eight paired and three unpaired neuropils including antennal lobes, optic lobe neuropils, mushroom body calyces and pedunculi, and central complex. For each of the two standard brains, we averaged brain areas of 20 individual brains. Additionally, we characterized eight selected olfactory glomeruli from 10 A0 female and male beetles respectively, which we could unequivocally recognize from individual to individual owing to their size and typical position in the antennal lobes. In summary, comparison of the averaged neuropil volumes revealed no sexual dimorphism in any of the reconstructed neuropils in A0 Tribolium brains. Both, the female and male 3D standard brain are also used for interspecies comparisons, and, very importantly, will serve as future volumetric references after genetical manipulation especially regarding metamorphic development and adult plasticity.

  4. Innexin7a forms junctions that stabilize the basal membrane during cellularization of the blastoderm in Tribolium castaneum.

    Science.gov (United States)

    van der Zee, Maurijn; Benton, Matthew A; Vazquez-Faci, Tania; Lamers, Gerda E M; Jacobs, Chris G C; Rabouille, Catherine

    2015-06-15

    In insects, the fertilized egg undergoes a series of rapid nuclear divisions before the syncytial blastoderm starts to cellularize. Cellularization has been extensively studied in Drosophila melanogaster, but its thick columnar blastoderm is unusual among insects. We therefore set out to describe cellularization in the beetle Tribolium castaneum, the embryos of which exhibit a thin blastoderm of cuboidal cells, like most insects. Using immunohistochemistry, live imaging and transmission electron microscopy, we describe several striking differences to cellularization in Drosophila, including the formation of junctions between the forming basal membrane and the yolk plasmalemma. To identify the nature of this novel junction, we used the parental RNAi technique for a small-scale screen of junction proteins. We find that maternal knockdown of Tribolium innexin7a (Tc-inx7a), an ortholog of the Drosophila gap junction gene Innexin 7, leads to failure of cellularization. In Inx7a-depleted eggs, the invaginated plasma membrane retracts when basal cell closure normally begins. Furthermore, transiently expressed tagged Inx7a localizes to the nascent basal membrane of the forming cells in wild-type eggs. We propose that Inx7a forms the newly identified junctions that stabilize the forming basal membrane and enable basal cell closure. We put forward Tribolium as a model for studying a more ancestral mode of cellularization in insects.

  5. The ABCs of eye color in Tribolium castaneum: orthologs of the Drosophila white, scarlet, and brown Genes.

    Science.gov (United States)

    Grubbs, Nathaniel; Haas, Sue; Beeman, Richard W; Lorenzen, Marcé D

    2015-03-01

    In Drosophila melanogaster, each of the three paralogous ABC transporters, White, Scarlet and Brown, is required for normal pigmentation of the compound eye. We have cloned the three orthologous genes from the beetle Tribolium castaneum. Conceptual translations of Tribolium white (Tcw), scarlet (Tcst), and brown (Tcbw) are 51, 48, and 32% identical to their respective Drosophila counterparts. We have identified loss-of-eye-pigment strains that bear mutations in Tcw and Tcst: the Tcw gene in the ivory (i) strain carries a single-base transversion, which leads to an E → D amino-acid substitution in the highly conserved Walker B motif, while the Tcst gene in the pearl (p) strain has a deletion resulting in incorporation of a premature stop codon. In light of these findings, the mutant strains i and p are herein renamed white(ivory) (w(i)) and scarlet(pearl) (st(p)), respectively. In addition, RNA inhibition of Tcw and Tcst recapitulates the mutant phenotypes, confirming the roles of these genes in normal eye pigmentation, while RNA interference of Tcbw provides further evidence that it has no role in eye pigmentation in Tribolium. We also consider the evolutionary implications of our findings.

  6. A Tribolium castaneum whole-embryo culture protocol for studying the molecular mechanisms and morphogenetic movements involved in insect development.

    Science.gov (United States)

    Macaya, Constanza C; Saavedra, Patricio E; Cepeda, Rodrigo E; Nuñez, Viviana A; Sarrazin, Andres F

    2016-01-01

    The development of the red flour beetle Tribolium castaneum is more representative of arthropods than the evolutionarily derived fly, Drosophila melanogaster. Thus, Tribolium is becoming an emerging organism model for studying the evolution of the mechanisms that control embryonic development in arthropods. In this regard, diverse genetic and molecular tools are currently available for Tribolium, as well as imaging and embryonic techniques. Recently, we developed a method for culturing embryos in order to study specific stages during Tribolium development. In this report, we present a detailed and "easy-to-follow" protocol for embryo handling and dissection, extending the use of whole-embryo culture to functional analysis by performing in vivo pharmacological manipulations. This experimental accessibility allowed us to study the relevance of microtubules in axis elongation, using nocodazole and taxol drugs to interfere with microtubule networks, followed by length measurement analysis. Additionally, we demonstrated that embryo handling had no effect on the development of Tribolium embryos, and we checked viability after dissection and bisection and during incubation using propidium iodide. The embryo culture protocol we describe here can be applied to study diverse developmental processes in Tribolium. We expect that this protocol can be adapted and applied to other arthropods.

  7. A Soluble Pyrophosphatase Is Essential to Oogenesis and Is Required for Polyphosphate Metabolism in the Red Flour Beetle (Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Klébea Carvalho

    2015-03-01

    Full Text Available Polyphosphates have been found in all cell types examined to date and play diverse roles depending on the cell type. In eukaryotic organisms, polyphosphates have been mainly investigated in mammalian cells with few studies on insects. Some studies have demonstrated that a pyrophosphatase regulates polyphosphate metabolism, and most of them were performed on trypanosomatids. Here, we investigated the effects of sPPase gene knocked down in oogenesis and polyphosphate metabolism in the red flour beetle (Tribolium castaneum. A single sPPase gene was identified in insect genome and is maternally provided at the mRNA level and not restricted to any embryonic or extraembryonic region during embryogenesis. After injection of Tc-sPPase dsRNA, female survival was reduced to 15% of the control (dsNeo RNA, and egg laying was completely impaired. The morphological analysis by nuclear DAPI staining of the ovarioles in Tc-sPPase dsRNA-injected females showed that the ovariole number is diminished, degenerated oocytes can be observed, and germarium is reduced. The polyphosphate level was increased in cytoplasmic and nuclear fractions in Tc-sPPase RNAi; Concomitantly, the exopolyphosphatase activity decreased in both fractions. Altogether, these data suggest a role for sPPase in the regulation on polyphosphate metabolism in insects and provide evidence that Tc-sPPase is essential to oogenesis.

  8. Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum

    Science.gov (United States)

    Walski, Tomasz; Van Damme, Els J. M.; Smargiasso, Nicolas; Christiaens, Olivier; De Pauw, Edwin; Smagghe, Guy

    2016-01-01

    In holometabolous insects the transition from larva to adult requires a complete body reorganization and relies on N-glycosylated proteins. N-glycosylation is an important posttranslational modification that influences protein activity but its impact on the metamorphosis has not been studied yet. Here we used the red flour beetle, Tribolium castaneum, to perform a first comprehensive study on the involvement of the protein N-glycosylation pathway in metamorphosis. The transcript levels for genes encoding N-glycan processing enzymes increased during later developmental stages and, in turn, transition from larva to adult coincided with an enrichment of more extensively modified paucimannose glycans, including fucosylated ones. Blockage of N-glycan attachment resulted in larval mortality, while RNAi of α-glucosidases involved in early N-glycan trimming and quality control disrupted the larva to pupa transition. Additionally, simultaneous knockdown of multiple genes responsible for N-glycan processing towards paucimannose structures revealed their novel roles in pupal appendage formation and adult eclosion. Our findings revealed that, next to hormonal control, insect post-embryonic development and metamorphosis depend on protein N-glycan attachment and efficient N-glycan processing. Consequently, disruption of these processes could be an effective new approach for insect control. PMID:27731363

  9. Experimental removal of sexual selection leads to decreased investment in an immune component in female Tribolium castaneum.

    Science.gov (United States)

    Hangartner, Sandra; Michalczyk, Łukasz; Gage, Matthew J G; Martin, Oliver Y

    2015-07-01

    Because of divergent selection acting on males and females arising from different life-history strategies, polyandry can be expected to promote sexual dimorphism of investment into immune function. In previous work we have established the existence of such divergence within populations where males and females are exposed to varying degrees of polyandry. We here test whether the removal of sexual selection via enforced monogamy generates males and females that have similar levels of investment in immune function. To test this prediction experimentally, we measured differences between the sexes in a key immune measurement (phenoloxidase (PO) activity) and resistance to the microsporidian Paranosema whitei in Tribolium castaneum lines that evolved under monogamous (sexual selection absent) vs polyandrous (sexual selection present) mating systems. At generation 49, all selected lines were simultaneously assessed for PO activity and resistance to their natural parasite P. whitei after two generations of relaxed selection. We found that the polyandrous regime was associated with a clear dimorphism in immune function: females had significantly higher PO activities than males in these lines. In contrast, there was no such difference between the sexes in the lines evolving under the monogamous regime. Survival in the infection experiment did not differ between mating systems or sexes. Removing sexual selection via enforced monogamy thus seems to erase intersexual differences in immunity investment. We suggest that higher PO activities in females that have evolved under sexual selection might be driven by the increased risk of infections and/or injuries associated with exposure to multiple males.

  10. Differences in Attack Avoidance and Mating Success between Strains Artificially Selected for Dispersal Distance in Tribolium castaneum.

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    Kentarou Matsumura

    Full Text Available Individuals of both dispersal and non-dispersal types (disperser and non-disperser are found in a population, suggesting that each type has both costs and benefits for fitness. However, few studies have examined the trade-off between the costs and benefits for the types. Here, we artificially selected for walking distance, i.e., an indicator of dispersal ability, in the red flour beetle Tribolium castaneum and established strains with longer (L-strains or shorter (S-strains walking distances. We then compared the frequency of predation by the assassin bug Amphibolus venator and the mating frequency of the selected strains. L-strain beetles suffered higher predation risk, than did S-strain beetles. L-strain males had significantly increased mating success compared to S-strain males, but females did not show a significant difference between the strains. The current results showed the existence of a trade-off between predation avoidance and mating success associated with dispersal types at a genetic level only in males. This finding can help to explain the maintenance of variation in dispersal ability within a population.

  11. Possible origin of B chromosome in Dichotomius sericeus (Coleoptera).

    Science.gov (United States)

    Amorim, Igor Costa; Milani, Diogo; Cabral-de-Mello, Diogo Cavalcanti; Rocha, Marília França; Moura, Rita Cássia

    2016-08-01

    B chromosomes have so far been described in about 80 species of Coleoptera, mainly using conventional staining analysis. In this study, 152 individuals of the dung beetle Dichotomius sericeus (Coleoptera), collected from three isolated geographical areas in the State of Pernambuco, Brazil, were analyzed to determine the frequency, prevalence, distribution, meiotic behavior, and possible B chromosome origin. The cytogenetic analysis consisted of conventional staining, C-banding, triple fluorochrome staining (CMA3/DA/DAPI), and fluorescent in situ hybridization using ribosomal DNAs (rDNAs) and H3 histone gene as probes, as well as microdissection and chromosome painting of the B chromosome. The B chromosomes were detected in all populations analyzed. Analysis revealed the heterochromatic nature and the presence of G+C-rich blocks and 18S rDNA on the B chromosome. FISH with DNA from microdissected B chromosome painted the entire extension of the B chromosome for all populations, besides the pericentromeric regions of all the autosomes, as well as the X chromosome. Finally, cross-hybridization in nine related species of Dichotomius using the microdissected B chromosome as probe did not reveal any hybridization signal. The results suggest an intraspecific and monophyletic origin for B chromosomes in D. sericeus, probably from the second or third autosomal pair.

  12. ON SEVEN OVERLOOKED CURCULIONOIDEA FROM NORTHERN ITALY (Coleoptera

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    Roberto Caldara

    2012-04-01

    Full Text Available Seven Curculionoidea described from Lombardy (northern Italy by Virginio Betta in 1857 and overlooked by subsequent authors are here treated. Since the Betta collection where their types are supposed to have been preserved was never traced, neotypes of all of them are designated with the purpose of clarifying their taxonomic status. Revised combinations are: Pseudeuparius pictus (Betta, 1857 comb. rev. from Tropideres Schoenherr, 1823; Pachytychius aericollis (Betta, 1857 comb. rev. from Smicronyx Schoenherr, 1843; Mecinus vittatus (Betta, 1857 comb. rev. from Gymnetron Schoenherr, 1825; Mecinus areolatus (Betta, 1857 comb. rev. from Gymnetron Schoenherr; Mecinus flavipes (Betta, 1857 comb. rev. from Gymnetron Schoenherr. The following new synonymies are established: Pseudeuparius centromaculatus (Gyllenhal, 1833 [= P. pictus (Betta, 1857 syn. n.]; Pachytychius sparsutus (Olivier, 1807 [= P. aericollis (Betta, 1857 syn. n.]; Smicronyx coecus (Reich, 1797 [= S. minusculus (Betta, 1857 syn. n.]; Mecinus labilis (Herbst, 1795 [= M. vittatus (Betta, 1857 syn. n.]; Mecinus pascuorum (Gyllenhal, 1813 [= M. areolatus (Betta, 1857 syn. n.]; Mecinus latiusculus (Jacquelin du Val, 1855 [= M. flavipes (Betta, 1857 syn. n.]; Mecinus circulatus (Marsham, 1802 [= M. sericatus Betta, 1857 syn. n.].

  13. Comparison of carboxylesterase acitivity between the resistant and susceptible strains of Tribolium castaneum to phosphine%赤拟谷盗的磷化氢敏感和抗性品系体内羧酸酯酶的活性比较

    Institute of Scientific and Technical Information of China (English)

    王殿轩; 原锴; 高希武

    2010-01-01

    本文比较测定了赤拟谷盗Tribolium castaneum(Herbst)的磷化氢抗性(Rf=327)和敏感品系害虫的羧酸酯酶活性,研究了该害虫同一品系不同个体间羧酸酯酶的活性差异,比较了两品系害虫在系列磷化氢浓度下熏蒸24 h和6.94×10-2mg/L磷化氢浓度下熏蒸不同时间的羧酸酯酶活性.主要结果为:未熏蒸的抗性害虫幼虫和蛹体内的羧酸酯酶活性分别高于敏感品系的1.37和1.16倍;敏感和抗性害虫同品系内不同个体间羧酸酯酶活性分布频率都存在明显差异,抗性害虫中酶活性大的个体数量占的比例较大;磷化氢浓度分别为0.69×10-2、2.78×10-2、5.56×10-2、8.33×10-2和11.11×10-2mg/L时都可导致敏感害虫羧酸酯酶的活性降低,但活性受抑制的程度不因浓度高低呈相应的增减.浓度分别为5.56×10-2、11.1l×10-2、13.89×10-2、20.83×10-2和27.78×10-2mg/L的熏蒸中抗性害虫体内酶活性增加,且活性增高的程度与浓度增减也不呈对应变化.在6.94×10-2mg/L磷化氢浓度下熏蒸不同时间的结果中,敏感害虫的酶活性随时间延长而下降,抗性害虫的活性则随时间延长而增大.研究表明赤拟谷盗对磷化氢的抗性可能与羧酸酯酶的活性增加有关.

  14. The Effect of Temperature and Humidity on the Susceptibility of Flour Beetles to Organophosphorus Insecticides

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    D. B. Chadha

    1965-01-01

    Full Text Available The effect of the temperature and humidity on the susceptibility of Tribolium castaneum Herbst. to malathion and diazinon, has been investigated. It has been found that temperature significantly influences the susceptibility of T. castaneum to malathion though not to diazinon. Humidity, however, influences the susceptibility if the insect to both insecticides.

  15. The roles of thermal transient receptor potential channels in thermotactic behavior and in thermal acclimation in the red flour beetle, Tribolium castaneum.

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    Kim, Hong Geun; Margolies, David; Park, Yoonseong

    2015-05-01

    To survive in variable or fluctuating temperature, organisms should show appropriate behavioral and physiological responses which must be mediated through properly attuned thermal sensory mechanisms. Transient receptor potential channels (TRPs) are a family of cation channels a number of which, called thermo-TRPs, are known to function as thermosensors. We investigated the potential role of thermo-TPRs that have been previously identified in the fruit fly, Drosophila melanogaster, in thermotaxis and thermal acclimation in the red flour beetle, Tribolium castaneum. Phylogenetic analysis of the trp genes showed generally one-to-one orthology between those in D. melanogaster and in T. castaneum, although there are putative gene-losses in two TRP subfamilies of D. melanogaster. With RNA interference (RNAi) of T. castaneum thermo-TRP candidates painless, pyrexia and trpA1, we measured thermal avoidance behavior. RNAi of trpA1 resulted in reduced avoidance of high temperatures, 39 and 42 °C. We also measured the effects of RNAi on heat-induced knockout and death under a short exposure to high temperature (1min at 52 °C) either with or without a 10-min acclimation period at 42 °C. Relatively short exposure to high temperature was enough to induce high temperature thermal acclimation. RNAi of trpA1 led to faster knockout at 52 °C. RNAi of painless showed lower recovery rates from heat-induced knockout after thermal acclimation, and RNAi of pyrexia showed lower long-term survivorship without thermal acclimation. Therefore, we concluded that trpA1 is important in high temperature sensing and also in enhanced tolerance to high-temperature induced knockout; painless plays a role in rapid acclimation to high temperature; and pyrexia functions in protecting beetles from acute heat stress without acclimation.

  16. Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum

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    Xiao Liang

    2015-01-01

    Full Text Available Some cytochrome P450 (CYP genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively, permethrin (2.00- and 2.03-fold and lambda-cyhalothrin (1.73- and 1.81-fold, whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

  17. Dissecting systemic RNA interference in the red flour beetle Tribolium castaneum: parameters affecting the efficiency of RNAi.

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    Sherry C Miller

    Full Text Available The phenomenon of RNAi, in which the introduction of dsRNA into a cell triggers the destruction of the corresponding mRNA resulting in a gene silencing effect, is conserved across a wide array of plant and animal phyla. However, the mechanism by which the dsRNA enters a cell, allowing the RNAi effect to occur throughout a multicellular organism (systemic RNAi, has only been studied extensively in certain plants and the nematode Caenorhabditis elegans. In recent years, RNAi has become a popular reverse genetic technique for gene silencing in many organisms. Although many RNAi techniques in non-traditional model organisms rely on the systemic nature of RNAi, little has been done to analyze the parameters required to obtain a robust systemic RNAi response. The data provided here show that the concentration and length of dsRNA have profound effects on the efficacy of the RNAi response both in regard to initial efficiency and duration of the effect in Tribolium castaneum. In addition, our analyses using a series of short dsRNAs and chimeric dsRNA provide evidence that dsRNA cellular uptake (and not the RNAi response itself is the major step affected by dsRNA size in Tribolium. We also demonstrate that competitive inhibition of dsRNA can occur when multiple dsRNAs are injected together, influencing the effectiveness of RNAi. These data provide specific information essential to the design and implementation of RNAi based studies, and may provide insight into the molecular basis of the systemic RNAi response in insects.

  18. Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum.

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    Minakuchi, Chieka; Ishii, Fumika; Washidu, Yumiko; Ichikawa, Akio; Tanaka, Toshiharu; Miura, Ken; Shinoda, Tetsuro

    2015-09-01

    Juvenile hormone (JH) is synthesized and secreted by the corpora allata. In the final two steps of JH biosynthesis, farnesoic acid (FA) is converted to JH through methylation by JH acid O-methyltransferase (JHAMT) and epoxidation by the cytochrome P450 enzyme CYP15. In the present study, we identified a homolog of CYP15 from the red flour beetle Tribolium castaneum (TcCYP15A1), and analyzed its expression as well as its role in JH biosynthesis. Quantitative RT-PCR analysis showed that the level of TcCYP15A1 mRNA was high in the embryonic stage as well as in the middle of the final larval instar. In the embryonic stage, the transcript level of TcCYP15A1 started to increase 30h after egg laying (AEL), peaked 54-60h AEL, and was followed by an increase of TcJHAMT mRNA, suggesting that JH biosynthesis started at this time point. TcCYP15A1 mRNA was present, but not exclusively so in the larval corpora allata. The recombinant TcCYP15A1 protein epoxidized both FA and methyl farnesoate (MF) in highly stereo-specific manners. These results confirmed that TcCYP15A1 is involved in JH biosynthesis. The RNAi-mediated knockdown of TcCYP15A1 in the pre-final larval instar did not result in precocious metamorphosis to pupa, indicating that MF may exhibit JH-like activity in order to maintain the larval status. The double knockdown of TcJHAMT and TcCYP15A1 resulted in pupae and adults with shorter wings, suggesting that the precursors of JH, JH acid and MF, may be essential for wing expansion.

  19. Insecticide-mediated up-regulation of cytochrome P450 genes in the red flour beetle (Tribolium castaneum).

    Science.gov (United States)

    Liang, Xiao; Xiao, Da; He, Yanping; Yao, Jianxiu; Zhu, Guonian; Zhu, Kun Yan

    2015-01-19

    Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

  20. A Major Facilitator Superfamily protein encoded by TcMucK gene is not required for cuticle pigmentation, growth and development in Tribolium castaneum.

    Science.gov (United States)

    Mun, Seulgi; Noh, Mi Young; Osanai-Futahashi, Mizuko; Muthukrishnan, Subbaratnam; Kramer, Karl J; Arakane, Yasuyuki

    2014-06-01

    Insect cuticle pigmentation and sclerotization (tanning) are vital physiological processes for insect growth, development and survival. We have previously identified several colorless precursor molecules as well as enzymes involved in their biosynthesis and processing to yield the mature intensely colored body cuticle pigments. A recent study indicated that the Bombyx mori (silkmoth) gene, BmMucK, which encodes a protein orthologous to a Culex pipiens quiquefasciatus (Southern house mosquito) cis,cis, muconate transporter, is a member of the "Major Facilitator Superfamily" (MFS) of transporter proteins and is associated with the appearance of pigmented body segments of naturally occurring body color mutants of B. mori. While RNA interference of the BmMucK gene failed to result in any observable phenotype, RNAi using a dsRNA for an orthologous gene from the red flour beetle, Tribolium castaneum, was reported to result in molting defects and darkening of the cuticle and some body parts, leading to the suggestion that orthologs of MucK genes may differ in their functions among insects. To verify the role and essentiality of the ortholog of this gene in development and body pigmentation function in T. castaneum we obtained cDNAs for the orthologous gene (TcMucK) from RNA isolated from the GA-1 wild-type strain of T. castaneum. The sequence of a 1524 nucleotides-long cDNA for TcMucK which encodes the putatively full-length protein, was assembled from two overlapping RT-PCR fragments and the expression profile of this gene during development was analyzed by real-time PCR. This cDNA encodes a 55.8 kDa protein consisting of 507 amino acid residues and includes 11 putative transmembrane segments. Transcripts of TcMucK were detected throughout all of the developmental stages analyzed. The function of this gene was explored by injection of two different double-stranded RNAs targeting different regions of the TcMucK gene (dsTcMucKs) into young larvae to down

  1. Vertical stratification of beetles (Coleoptera) and flies (Diptera) in temperate forest canopies.

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    Maguire, Dorothy Y; Robert, Katleen; Brochu, Kristen; Larrivée, Maxim; Buddle, Christopher M; Wheeler, Terry A

    2014-02-01

    Forest canopies support high arthropod biodiversity, but in temperate canopies, little is known about the spatial distribution of these arthropods. This is an important first step toward understanding ecological roles of insects in temperate canopies. The objective of this study was to assess differences in the species composition of two dominant and diverse taxa (Diptera and Coleoptera) along a vertical gradient in temperate deciduous forest canopies. Five sugar maple trees from each of three deciduous forest sites in southern Quebec were sampled using a combination of window and trunk traps placed in three vertical strata (understory, mid-canopy, and upper-canopy) for three sampling periods throughout the summer. Coleoptera species richness and abundance did not differ between canopy heights, but more specimens and species of Diptera were collected in the upper-canopy. Community composition of Coleoptera and Diptera varied significantly by trap height. Window traps collected more specimens and species of Coleoptera than trunk traps, although both trap types should be used to maximize representation of the entire Coleoptera community. There were no differences in abundance, diversity, or composition of Diptera collected between trap types. Our data confirm the relevance of sampling all strata in a forest when studying canopy arthropod biodiversity. PMID:24472199

  2. Vertical stratification of beetles (Coleoptera) and flies (Diptera) in temperate forest canopies.

    Science.gov (United States)

    Maguire, Dorothy Y; Robert, Katleen; Brochu, Kristen; Larrivée, Maxim; Buddle, Christopher M; Wheeler, Terry A

    2014-02-01

    Forest canopies support high arthropod biodiversity, but in temperate canopies, little is known about the spatial distribution of these arthropods. This is an important first step toward understanding ecological roles of insects in temperate canopies. The objective of this study was to assess differences in the species composition of two dominant and diverse taxa (Diptera and Coleoptera) along a vertical gradient in temperate deciduous forest canopies. Five sugar maple trees from each of three deciduous forest sites in southern Quebec were sampled using a combination of window and trunk traps placed in three vertical strata (understory, mid-canopy, and upper-canopy) for three sampling periods throughout the summer. Coleoptera species richness and abundance did not differ between canopy heights, but more specimens and species of Diptera were collected in the upper-canopy. Community composition of Coleoptera and Diptera varied significantly by trap height. Window traps collected more specimens and species of Coleoptera than trunk traps, although both trap types should be used to maximize representation of the entire Coleoptera community. There were no differences in abundance, diversity, or composition of Diptera collected between trap types. Our data confirm the relevance of sampling all strata in a forest when studying canopy arthropod biodiversity.

  3. BESOUROS COPRÓFAGOS (COLEOPTERA; SCARABAEIDAE COLETADOS EM PIRACICABA, SP

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    RODRIGUES S.R.

    1998-01-01

    Full Text Available Através do uso de armadilhas "pitfall" iscadas com massa fecal fresca de bovinos, realizou-se a coleta de besouros coprófagos (Coleoptera; Scarabaeidae, durante o período de 15 de abril de 1995 a 17 de fevereiro de 1996, em área de pastagem ao lado de confinamento de bovinos, em Piracicaba, SP. Coletou-se um total de 11 espécies distribuidas nos gêneros Aphodius, Ataenius, Trichillum, Eurysternus, Dichotomius e Canthon. Os besouros coprófagos de comportamento endocoprídeo representaram 72,73% das espécies coletadas, sendo estes os de maior ocorrência e a espécie Aphodius lividus parece ser a mais bem adaptada na área estudada.

  4. Brachylophora, a new brachypterous genus of Rhopalophorini (Coleoptera, Cerambycidae

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    Robin O. S. Clarke

    2011-06-01

    Full Text Available Brachylophora, a new brachypterous genus of Rhopalophorini (Coleoptera, Cerambycidae. Brachylophora auricollis (Bruch, 1918 comb. nov. = Pasiphyle auricollis Bruch, 1918, originally described from Argentina (Salta, is redescribed and illustrated. Although with reduced elytra, the genus is transferred from Rhinotragini to Rhopalophorini based on the following characters: eyes well separated in both sexes, frons between eyes depressed and lacking frontal suture; pro-, meso-, and metasternum planar; mesothorax parallel-sided, not at all declivous before mesosternal process; metasternum large, together with mesosternum twice length of prosternum, metepisternum very wide, entire suture separating it from metasternum clearly visible when viewed from below; female ovipositor shortened with short cylindrical styles; and, more generally, structural features of hind legs, and surface ornamentation. Habitus similar to Coremia group. Bolivian specimens were netted as they visited flowers of Croton sp. (Euphorbiaceae.

  5. Coleoptera associated with macrophytes of the genus Salvinia in four oxbow lakes in two river basins in southeast Brazil.

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    Paula-Bueno, M C; Fonseca-Gessner, A A

    2015-11-01

    Macrophytes in oxbow lakes represent an important substrate for the Coleoptera. Two oxbow lakes the Rio Paranapanema were studied and the other two Rio Mogi-Guaçu, in the State de São Paulo, Brasil. In this study, there is greater similarity between the communities of Coleoptera of lakes greater connectivity with the main river channel or the difference in the species of Salvinia collected in the lakes studied interferes Coleoptera fauna that uses as substrate. A total of 9,222 specimens of Coleoptera were collected and identified in 10 families and 40 genera. The analysis MDS for abundance of Coleoptera showed the grouping of the oxbow lakes the Paranapanema River and a distancing the oxbow lakes the Mogi-Guaçu. The PERMANOVA test did not reveal any difference in the fauna between the wet and dry periods. It was concluded that the connectivity between river and lake is not decisive for the richness and abundance of aquatic fauna of Coleoptera. Therefore, the richness and abundance of aquatic Coleoptera associated vary with the species of Salvinia used as substrate.

  6. Coleoptera associated with macrophytes of the genus Salvinia in four oxbow lakes in two river basins in southeast Brazil.

    Science.gov (United States)

    Paula-Bueno, M C; Fonseca-Gessner, A A

    2015-11-01

    Macrophytes in oxbow lakes represent an important substrate for the Coleoptera. Two oxbow lakes the Rio Paranapanema were studied and the other two Rio Mogi-Guaçu, in the State de São Paulo, Brasil. In this study, there is greater similarity between the communities of Coleoptera of lakes greater connectivity with the main river channel or the difference in the species of Salvinia collected in the lakes studied interferes Coleoptera fauna that uses as substrate. A total of 9,222 specimens of Coleoptera were collected and identified in 10 families and 40 genera. The analysis MDS for abundance of Coleoptera showed the grouping of the oxbow lakes the Paranapanema River and a distancing the oxbow lakes the Mogi-Guaçu. The PERMANOVA test did not reveal any difference in the fauna between the wet and dry periods. It was concluded that the connectivity between river and lake is not decisive for the richness and abundance of aquatic fauna of Coleoptera. Therefore, the richness and abundance of aquatic Coleoptera associated vary with the species of Salvinia used as substrate. PMID:26602356

  7. Diversidad de la familia Carabidae (Coleoptera en Chile Diversity of the family Carabidae (Coleoptera in Chile

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    SERGIO ROIG-JUÑENT

    2001-09-01

    Full Text Available Carabidae constituye dentro de los coléopteros chilenos, la cuarta familia en importancia por su cantidad de especies. El presente trabajo incluye una breve compilación acerca de la historia de la familia y de las primeras expediciones realizadas en Chile. También se realizan comparaciones de la diversidad de carábidos chilenos con respecto a otros países y el Neotrópico. Para Chile, se conocen 21 tribus, con 95 géneros y 365 especies, que representan el 38,8, 28,8 y 7,9 % de la fauna del Neotrópico, respectivamente. Chile posee un bajo número de tribus comparado con otros países, sin embargo, constituye un área importante por la presencia de seis tribus relictuales, principalmente pangeicas o gondwánicas. Chile posee 18 géneros endémicos (18,5 % de su fauna de Carabidae, 28 cuya distribución está restringida a Chile y Argentina y seis restringidos a Chile, Argentina y Uruguay. La cantidad de especies presentes en Chile es inferior a la que poseen otros países de América del Sur, pero la cantidad de especies endémicas es muy alta (204 y representa el 55,8 % de su fauna de carábidos. El alto grado de endemismo que posee Chile con respecto a otros países de América del Sur puede deberse a su condición de aislamiento, siendo las barreras más importantes la región desértica del norte y la cordillera de Los Andes. Este hecho también se vislumbra por la ausencia de importantes tribus neotropicales como Galeritini, Scaritini y Brachinini. También se incluyen en este trabajo claves para la identificación de todas las tribus y géneros presentes en Chile, como así también una breve descripción acerca de la diversidad y ambientes en los que se encuentra cada géneroThe family Carabidae is the fourth largest Coleoptera family in Chile. The present work includes a brief compilation on the taxonomic history of the family and the first expeditions to Chile. In addition, knowledge of carabid diversity in Chile is compared with

  8. Track analysis of the Neotropical Entimini (Coleoptera: Curculionidae: Entiminae

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    Alberto Romo

    2011-09-01

    Full Text Available Track analysis of the Neotropical Entimini (Coleoptera, Curculionidae, Entiminae. Distributional patterns of the species belonging to the tribe Entimini from the Neotropical region were analyzed. Based on a track analysis of 22 species of Entimus, Rhigus, and Phaedropus, for which distributional data were available, two generalized tracks were found. One is located in northern Brazil, corresponding to the Amazonian subregion, and is determined by Phaedropus candidus and Rhigus speciosus. The other is located in southern Brazil, corresponding to the Parana subregion, and is determined by Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus, and R. tribuloides. The development of the Chacoan subregion is hypothesized to have been the dynamic vicariant event that fragmented the former Amazonian-Parana forest.Análisis de trazos de Entimini Neotropical (Coleoptera, Curculionidae, Entiminae. Se analizaron los patrones de distribución de la tribu Entimini en la región Neotropical. Con base en un análisis de los trazos de 22 especies de Entimus, Rhigus y Phaedropus, para los cuales se contaba con datos de distribución, se hallaron dos trazos generalizados. Uno se localiza en el norte de Brasil, en la subregión Amazónica, y está sustentado por Phaedropus candidus y Rhigus speciosus. El otro se localiza en el sur de Brasil, en la subregión Paranaense, y está sustentado por Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus y R. tribuloides. Se hipotetiza que el desarrollo de la subregión Chaqueña constituyó el evento de vicarianza dinámica que fragmentó el antiguo bosque Amazónico-Paranaense.

  9. Diversity of forensic rove beetles (Coleoptera, Staphylinidae) associated with decaying pig carcass in a forest biotope.

    Science.gov (United States)

    Dekeirsschieter, Jessica; Frederick, Christine; Verheggen, Francois J; Drugmand, Didier; Haubruge, Eric

    2013-07-01

    Most forensic studies are focused on Diptera pattern colonization while neglecting Coleoptera succession. So far, little information is available on the postmortem colonization by beetles and the decomposition process they initiate under temperate biogeoclimatic countries. These beetles have, however, been referred to as being part of the entomofaunal colonization of a dead body. Forensic entomologists need increased databases detailing the distribution, ecology, and phenology of necrophagous insects, including staphylinids (Coleoptera, Staphylinidae). While pig carcasses are commonly used in forensic entomology studies to surrogate human decomposition and to investigate the entomofaunal succession, very few works have been conducted in Europe on large carcasses. Our work reports the monitoring of the presence of adult rove beetles (Coleoptera, Staphylinidae) on decaying pig carcasses in a forest biotope during four seasons (spring, summer, fall, and winter). A total of 23 genera comprising 60 species of rove beetles were collected from pig carcasses.

  10. Genomic and gene regulatory signatures of cryptozoic adaptation: Loss of blue sensitive photoreceptors through expansion of long wavelength-opsin expression in the red flour beetle Tribolium castaneum

    Directory of Open Access Journals (Sweden)

    Cook Tiffany A

    2007-12-01

    Full Text Available Abstract Background Recent genome sequence analysis in the red flour beetle Tribolium castaneum indicated that this highly crepuscular animal encodes only two single opsin paralogs: a UV-opsin and a long wavelength (LW-opsin; however, these animals do not encode a blue (B-opsin as most other insects. Here, we studied the spatial regulation of the Tribolium single LW- and UV-opsin gene paralogs in comparison to that of the five opsin paralogs in the retina of Drosophila melanogaster. Results In situ hybridization analysis reveals that the Tribolium retina, in contrast with other insect retinas, constitutes a homogenous field of ommatidia that have seven LW-opsin expressing photoreceptors and one UV-/LW-opsin co-expressing photoreceptor per eye unit. This pattern is consistent with the loss of photoreceptors sensitive to blue wavelengths. It also identifies Tribolium as the first example of a species in insects that co-expresses two different opsins across the entire retina in violation of the widely observed "one receptor rule" of sensory cells. Conclusion Broader studies of opsin evolution in darkling beetles and other coleopteran groups have the potential to pinpoint the permissive and adaptive forces that played a role in the evolution of vision in Tribolium castaneum.

  11. Tribolium castaneum immune defense genes are differentially expressed in response to Bacillus thuringiensis toxins sharing common receptor molecules and exhibiting disparate toxicity.

    Science.gov (United States)

    Contreras, Estefanía; Benito-Jardón, María; López-Galiano, M José; Real, M Dolores; Rausell, Carolina

    2015-06-01

    In Tribolium castaneum larvae we have demonstrated by RNA interference knockdown that the Bacillus thuringiensis Cry3Ba toxin receptors Cadherin-like and Sodium solute symporter proteins are also functional receptors of the less active Cry3Aa toxin. Differences in susceptibility to B. thuringiensis infection might not only rely on toxin-receptor interaction but also on host defense mechanisms. We compared the expression of the immune related genes encoding Apolipophorin-III and two antimicrobial peptides, Defensin3 and Defensin2 after B. thuringiensis challenge. All three genes were up-regulated following Cry3Ba spore-crystal intoxication whereas only Defensins gene expression was induced upon Cry3Aa spore-crystal treatment, evidencing a possible association between host immune response and larval susceptibility to B. thuringiensis. We assessed the antimicrobial activity spectra of T. castaneum defensins peptide fragments and found that a peptide fragment of Defensin3 was effective against the human microbial pathogens, Escherichia coli, Staphylococcus aureus and Candida albicans, being S. aureus the most susceptible one.

  12. Influence of growing location and cultivar on Rhyzopertha dominica (Coleoptera: Bostrichidae) and Sitophilus oryzae (Coleoptera: Curculionidae) infestation of rough rice

    Institute of Scientific and Technical Information of China (English)

    FRANK H. ARTHUR; RUSTY C. BAUTISTA; TERRENCE J. SIEBENMORGEN

    2007-01-01

    Long-grain rice cultivars Cocodrie, Wells, and XP 723 grown in three locations (Hazen, MO; Essex and Newport, AR, USA), and medium-grain rice cultivars Bengal and XP 713 grown in two locations (Jonesboro and Lodge Corner, AR, USA), were harvested and assayed for susceptibility to Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), the lesser grain borer, and Sitophilus oryzae (L.) (Coleoptera: Curculionidae), the rice weevil, on rice held at 27℃ 57% and 75% relative humidity (RH).Separate samples from the same harvest lots were also analyzed for the physical characteristics of brown rice yield, percentage whole kernels and kernel thickness. Progeny production and feeding damage of R. dominica were significantly different among long-grain cultivars within two of the three locations (P < 0.05), but not for location or RH (P ≥ 0.05), while progeny production of S. oryzae was different among cultivars, location, and RH (P < 0.05). On medium-grain rice, both cultivar and location were significant for progeny production of R. dominica, but not RH, while cultivar and RH were significant for progeny production of S. oryzae, but not location. On both rice types, feeding damage of R. dominica followed the same trends and was always strongly positively correlated with progeny production (P < 0.05), but for S. oryzae there were several instances in which progeny production was not correlated with feeding damage (P ≥ 0.05). Physical characteristics of both rice types were statistically significant (P < 0.01) but actual numerical differences were extremely small, and were generally not correlated with progeny production of either species. Results indicate that the location in which a particular rice cultivar is grown, along with its characteristics, could affect susceptibility of the rice to R. dominica and S. oryzae.

  13. Coleoptera species inhabiting prairie wetlands of the Cottonwood Lake Area, Stutsman County, North Dakota

    Science.gov (United States)

    Hanson, B.A.; Swanson, G.A.

    1989-01-01

    The aquatic Coleoptera of a prairie wetland complex in Stutsman County, North Dakota, were collected from April 1979 to November 1980. Identification of 2594 individuals confirmed 57 species, including seven new records for North Dakota. Two seasonally flooded and two semipermanent wetlands, totaling 7.43 ha, contained 53% of the Dytiscidae, 43% of the Haliplidae, 38% of the Hydrophilidae, and 22% of the Gyrinidae species previously identified from North Dakota. Although 49.1% of the Coleoptera species occurred in both types of wetlands, the occurrence of 29 species varied by wetland class.

  14. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae)

    OpenAIRE

    Dilma Solange Napp; Ubirajara Ribeiro Martins

    2006-01-01

    Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae). Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae). The following new genus and species, all from Bolivia, are describ...

  15. Complex adaptive responses during antagonistic coevolution between Tribolium castaneum and its natural parasite Nosema whitei revealed by multiple fitness components

    Directory of Open Access Journals (Sweden)

    Bérénos Camillo

    2012-01-01

    Full Text Available Abstract Background Host-parasite coevolution can lead to local adaptation of either parasite or host if there is specificity (GxG interactions and asymmetric evolutionary potential between host and parasite. This has been demonstrated both experimentally and in field studies, but a substantial proportion of studies fail to detect such clear-cut patterns. One explanation for this is that adaptation can be masked by counter-adaptation by the antagonist. Additionally, genetic architecture underlying the interaction is often highly complex thus preventing specific adaptive responses. Here, we have employed a reciprocal cross-infection experiment to unravel the adaptive responses of two components of fitness affecting both parties with different complexities of the underlying genetic architecture (i.e. mortality and spore load. Furthermore, our experimental coevolution of hosts (Tribolium castaneum and parasites (Nosema whitei included paired replicates of naive hosts from identical genetic backgrounds to allow separation between host- and parasite-specific responses. Results In hosts, coevolution led to higher resistance and altered resistance profiles compared to paired control lines. Host genotype × parasite genotype interactions (GH × GP were observed for spore load (the trait of lower genetic complexity, but not for mortality. Overall parasite performance correlated with resistance of its matching host coevolution background reflecting a directional and unspecific response to strength of selection during coevolution. Despite high selective pressures exerted by the obligatory killing parasite, and host- and parasite-specific mortality profiles, no general pattern of local adaptation was observed, but one case of parasite maladaptation was consistently observed on both coevolved and control host populations. In addition, the use of replicate control host populations in the assay revealed one case of host maladaptation and one case of parasite

  16. 甲酸乙酯对面粉中赤拟谷盗的熏蒸效果%The fumigation effect of Ethyl formate against Triboloum castaneum in flour.

    Institute of Scientific and Technical Information of China (English)

    游源浅; 詹开瑞; 张晓燕; 陈艳

    2011-01-01

    The purpose of this research is to evaluate the fumigation activity of Ethyl formate against Tribolium castaneum. The Lct99 of T. castaneum was calculated by detecting concentration of Ethyl formate during exposure. The results indicate that Ethyl formate was effective in disinfecting all life stage of T. castaneum in flour, and the sensitivities of life stages of T. castaneum to Ethyl formate, which arranged from strongest to weakest, were egg, low instar larva, adult, high instar larva and pupa. The Lct99 for disinfecting all life stages of T. castaneum in 6 and 12 h at 23℃ were 448.42 and 599.34rag.h/L, respectively.%本文研究了甲酸乙酯对面粉中赤拟谷盗不同虫态的熏蒸作用。通过对熏蒸期间熏蒸剂浓度检测,计算浓度时间乘积(Cr)计算不同时间下甲酸乙酯对赤拟谷盗各虫态达到99%控制的处理指标(Lct99)。结果表明甲酸乙酯熏蒸能有效防治面粉中的赤拟谷盗各虫态,赤拟谷盗不同虫态对甲酸乙酯的敏感性由强到弱依次为:卵〉低龄幼虫〉成虫〉高龄幼虫〉蛹。在23℃条件下处理6h和12h对面粉中赤拟谷盗的厶‰分别为448.42和599.34mg·h/L

  17. Comparison of Coleoptera emergent from various decay classes of downed coarse woody debris in Great Smoky Mountains National Park, USA

    OpenAIRE

    Ferro, Michael L.; Gimmel, Matthew L.; Harms, Kyle E.; Carlton, Christopher E.

    2013-01-01

    Coleoptera species composition and succession in downed woody debris habitats are poorly known in eastern North America. A photoeclector emergence chamber was used to concentrate Coleoptera that emerged from various decay classes of fine and coarse woody debris (FWD and CWD, respectively) collected in primary and secondary forest sites in Great Smoky Mountains National Park, Tennessee, USA. A total of 5673 adult beetle specimens, representing 305 lowest identifiable taxa within 227 genera and...

  18. Contribution to the knowledge of seed-beetles (Coleoptera, Chrysomelidae, Bruchinae) in Xinjiang, China

    Science.gov (United States)

    Li, You; Wang, Zhiliang; Guo, Jianjun; Nápoles, Jesús Romero; Ji, Yingchao; Jiang, Chunyan; Zhang, Runzhi

    2014-01-01

    Abstract Nineteen species of seed-beetles belonging to the subfamily Bruchinae (Coleoptera, Chrysomelidae) were collected in Xinjiang, China. Of these, the following four were new records for China: Bruchus affinis Frolich, 1799, Bruchus atomarius L., 1761, Bruchus loti Paykull, 1800 and Kytorhinus kergoati Delobel & Legalov, 2009. We provide an annotated checklist, illustrations and a key to the 19 species. PMID:25610333

  19. Upper lethal temperature limits of the common furniture beetle Anobium punctatum (Coleoptera: Anobiidae)

    DEFF Research Database (Denmark)

    Hansen, Lise Stengård; Jensen, Karl-Martin Vagn

    1996-01-01

    The susceptibility of the egg, larval and adult stages of Anobium punctatum De Geer (Coleoptera: Anobiidae) to heat (46-54°C, 25-30% RH) was investigated. The larval stage was found to be most tolerant to heat. Very short exposure (5 min) of the larvae to temperatures of 52°C and above led to 100...

  20. New record of predatory ladybird beetle (Coleoptera, Coccinellidae feeding on extrafloral nectaries

    Directory of Open Access Journals (Sweden)

    Lúcia M. Almeida

    2011-09-01

    Full Text Available New record of predatory ladybird beetle (Coleoptera, Coccinellidae feeding on extrafloral nectaries. Feeding by Exoplectra miniata (Germar on extrafloral nectaries of Inga edulis Mart. was observed in Nova Friburgo, Rio de Janeiro, Brazil. This is the first record of this behavior for Exoplectrini.

  1. The genus Platytenerus Miyatake, 1985 (Coleoptera: Cleridae: Neorthopleurinae), with description of a new species from Japan.

    Science.gov (United States)

    Murakami, Hiroyuki

    2015-03-23

    The genus Platytenerus Miyatake, 1985 (Coleoptera: Cleridae) is redescribed and classified into the subfamily Neorthopleurinae Opitz, 2009. A phylogenetic tree is supplementally provided for Platytenerus based on twenty morphological and two geographical characters. A new species of the genus, Platytenerus iriomotensis sp. n. is described from Iriomote Island, Okinawa, Japan.

  2. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae from Korea

    Directory of Open Access Journals (Sweden)

    Ik Je Choi

    2016-03-01

    Full Text Available A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  3. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae) from Korea

    OpenAIRE

    Ik Je Choi; Jongok Lim; Jinyoung Park; Ji Hwan Park; Jong Kyun Park

    2016-01-01

    A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae) is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  4. Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae), information on a little known taxon from Colombia

    OpenAIRE

    Kippenhan, Mike

    2011-01-01

    Two specimens of Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae) in the Field Museum of Natural History offer additional information on the morphology and distribution of this rare species. One of the specimens, a female, is considered to be the first known specimen of this species.

  5. Diapause and post-diapause quiescence demonstrated in overwintering Harmonia axyeidis (Coleoptera: Coccinellidae) in northwestern Europe

    NARCIS (Netherlands)

    Raak-van den Berg, C.L.; Jong, de P.W.; Hemerik, L.; Lenteren, van J.C.

    2013-01-01

    The Asian ladybird Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) is regarded as an invasive species in many parts of the world. In a previous study we hypothesised that H. axyridis enters diapause at the end of October and then shifts to a quiescent state in December in northwestern Europe.

  6. ESPECIES DE DRYOPHTHORINAE (COLEOPTERA: CURCULIONIDAE ASOCIADAS A PLÁTANO Y BANANO (Musaspp. EN COLOMBIA Dryophthorinae Species (Coleoptera: Curculionidae Associted to Platain and Banana crops (Musaspp. in Colombia

    Directory of Open Access Journals (Sweden)

    PAULA A SEPÚLVEDA-CANO

    Full Text Available Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.This synopsis is about beetle&#’;s subfamily Dryophthorinae (Coleoptera: Curculionidae associated to plantain and banana crops. Additionally keys illustrated for the species of the country are offered. Six species associated to these cultures are registered: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.

  7. Molecular Markers Detect Cryptic Predation on Coffee Berry Borer (Coleoptera: Curculionidae) by Silvanid and Laemophloeid Flat Bark Beetles (Coleoptera: Silvanidae, Laemophloeidae) in Coffee Beans.

    Science.gov (United States)

    Sim, Sheina B; Yoneishi, Nicole M; Brill, Eva; Geib, Scott M; Follett, Peter A

    2016-02-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is a serious pest of coffee worldwide. It was first detected in Hawai'i in 2010. Two predatory beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and Leptophloeus sp. (Coleoptera: Laemophloeidae), have been observed in H. hampei-infested coffee. Under laboratory conditions, colony-reared C. quadricollis and Leptophloeus sp. prey upon all life stages of H. hampei. However, the H. hampei life cycle occurs almost exclusively within a coffee bean obscured from direct observation. Thus, it is unknown if C. quadricollis and Leptophloeus sp. consume H. hampei as prey in the wild. To demonstrate predation of H. hampei by C. quadricollis and Leptophloeus sp., a molecular assay was developed utilizing species-specific primers targeting short regions of the mitochondrial COI gene to determine species presence. Using these primers, wild C. quadricollis and Leptophloeus sp. were collected and screened for the presence of H. hampei DNA using PCR. Analysis of collections from five coffee farms revealed predation of C. quadricollis and Leptophloeus sp. on H. hampei. Further laboratory testing showed that H. hampei DNA could be detected in predators for as long as 48 h after feeding, indicating the farm-caught predators had preyed on H. hampei within 2 d of sampling. This study demonstrates the utility of molecular markers for the study of the ecology of predators and prey with cryptic behavior, and suggests C. quadricollis and Leptophloeus sp. might be useful biocontrol agents against H. hampei.

  8. Hox genes require homothorax and extradenticle for body wall identity specification but not for appendage identity specification during metamorphosis of Tribolium castaneum.

    Science.gov (United States)

    Smith, Frank W; Jockusch, Elizabeth L

    2014-11-01

    The establishment of segment identity is a key developmental process that allows for divergence along the anteroposterior body axis in arthropods. In Drosophila, the identity of a segment is determined by the complement of Hox genes it expresses. In many contexts, Hox transcription factors require the protein products of extradenticle (exd) and homothorax (hth) as cofactors to perform their identity specification functions. In holometabolous insects, segment identity may be specified twice, during embryogenesis and metamorphosis. To glean insight into the relationship between embryonic and metamorphic segmental identity specification, we have compared these processes in the flour beetle Tribolium castaneum, which develops ventral appendages during embryogenesis that later metamorphose into adult appendages with distinct morphologies. At metamorphosis, comparisons of RNAi phenotypes indicate that Hox genes function jointly with Tc-hth and Tc-exd to specify several region-specific aspects of the adult body wall. On the other hand, Hox genes specify appendage identities along the anteroposterior axis independently of Tc-hth/Tc-exd and Tc-hth/Tc-exd specify proximal vs. distal identity within appendages independently of Hox genes during this stage. During embryogenesis, Tc-hth and Tc-exd play a broad role in the segmentation process and are required for specification of body wall identities in the thorax; however, contrasting with results from other species, we did not obtain homeotic transformations of embryonic appendages in response to Tc-hth or Tc-exd RNAi. In general, the homeotic effects of interference with the function of Hox genes and Tc-hth/Tc-exd during metamorphosis did not match predictions based on embryonic roles of these genes. Comparing metamorphic patterning in T. castaneum to embryonic and post-embryonic development in hemimetabolous insects suggests that holometabolous metamorphosis combines patterning processes of both late embryogenesis and

  9. Two major cuticular proteins are required for assembly of horizontal laminae and vertical pore canals in rigid cuticle of Tribolium castaneum.

    Science.gov (United States)

    Noh, Mi Young; Kramer, Karl J; Muthukrishnan, Subbaratnam; Kanost, Michael R; Beeman, Richard W; Arakane, Yasuyuki

    2014-10-01

    The insect exoskeleton is composed of cuticle primarily formed from structural cuticular proteins (CPs) and the polysaccharide chitin. Two CPs, TcCPR27 and TcCPR18, are major proteins present in the elytron (highly sclerotized and pigmented modified forewing) as well as the pronotum (dorsal sclerite of the prothorax) and ventral abdominal cuticle of the red flour beetle, Tribolium castaneum. Both CPs belong to the CPR family, which includes proteins that have an amino acid sequence motif known as the Rebers & Riddiford (R&R) consensus sequence. Injection of double-stranded RNA (dsRNA) for TcCPR27 and TcCPR18 resulted in insects with shorter, wrinkled, warped and less rigid elytra than those from control insects. To gain a more comprehensive understanding of the roles of CPs in cuticle assembly, we analyzed for the precise localization of TcCPR27 and the ultrastructural architecture of cuticle in TcCPR27- and TcCPR18-deficient elytra. Transmission electron microscopic analysis combined with immunodetection using gold-labeled secondary antibody revealed that TcCPR27 is present in dorsal elytral procuticle both in the horizontal laminae and in vertical pore canals. dsRNA-mediated RNA interference (RNAi) of TcCPR27 resulted in abnormal electron-lucent laminae and pore canals in elytra except for the boundary between these two structures in which electron-dense molecule(s) apparently accumulated. Insects subjected to RNAi for TcCPR18 also had disorganized laminae and pore canals in the procuticle of elytra. Similar ultrastructural defects were also observed in other body wall regions with rigid cuticle such as the thorax and legs of adult T. castaneum. TcCPR27 and TcCPR18 are required for proper formation of the horizontal chitinous laminae and vertical pore canals that are critical for formation and stabilization of rigid adult cuticle.

  10. DNA barcoding of Japanese click beetles (Coleoptera, Elateridae).

    Science.gov (United States)

    Oba, Yuichi; Ôhira, Hitoo; Murase, Yukio; Moriyama, Akihiko; Kumazawa, Yoshinori

    2015-01-01

    Click beetles (Coleoptera: Elateridae) represent one of the largest groups of beetle insects. Some click beetles in larval form, known as wireworms, are destructive agricultural pests. Morphological identification of click beetles is generally difficult and requires taxonomic expertise. This study reports on the DNA barcoding of Japanese click beetles to enable their rapid and accurate identification. We collected and assembled 762 cytochrome oxidase subunit I barcode sequences from 275 species, which cover approximately 75% of the common species found on the Japanese main island, Honshu. This barcode library also contains 20 out of the 21 potential pest species recorded in Japan. Our analysis shows that most morphologically identified species form distinct phylogenetic clusters separated from each other by large molecular distances. This supports the general usefulness of the DNA barcoding approach for quick and reliable identification of Japanese elaterid species for environmental impact assessment, agricultural pest control, and biodiversity analysis. On the other hand, the taxonomic boundary in dozens of species did not agree with the boundary of barcode index numbers (a criterion for sequence-based species delimitation). These findings urge taxonomic reinvestigation of these mismatched taxa.

  11. The complete mitogenome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae).

    Science.gov (United States)

    Nan, Xiaoning; Wei, Cong; He, Hong

    2016-05-01

    The complete mitochondrial genome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae) were reconstructed from whole-genome Illumina Hiseq 2000 sequencing data with an average coverage of 1406.7X. The circular genome is 15,122 bp in length, consisting of 13 protein-coding genes (PCGs), 21 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and one D-loop or control region. The tRNA-Ile gene was not found in the mitochondrial genome, as is identical to two other curculionidae species, i.e. Sphenophorus sp. (GU176342) and Naupactus xanthographus (GU176345). All PCGs initiated with ATN codons, except for the ND1 started with TTG. Two PCGs (COI and ND4) have an incomplete stop codon T. Two PCGs (ND4L and ND1) harbor the stop codon TAG, while all other PCGs terminated with the TAA codon. The nucleotide composition is highly asymmetric (38.7% A, 14.4% C, 9.2% G and 37.8% T) with an overall AT content of 76.5%. PMID:25427809

  12. Plant compounds insecticide activity against Coleoptera pests of stored products

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Marcio Dionizio; Picanco, Marcelo Coutinho; Guedes, Raul Narciso Carvalho; Campos, Mateus Ribeiro de; Silva, Gerson Adriano; Martins, Julio Claudio [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Biologia Animal]. E-mail: marcio.dionizio@gmail.com; picanco@ufv.br; guedes@ufv.br; mateusc3@yahoo.com.br; agronomiasilva@yahoo.com.br

    2007-07-15

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD{sub 50} from 2.72 to 39.71 mg g{sup -1} a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis. (author)

  13. Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae

    Directory of Open Access Journals (Sweden)

    Elias Soares Gomes

    2014-06-01

    Full Text Available Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae. Coleopterans of the family Melolonthidae comprise a large group of species that feed on different food sources, including plant roots, stems, and leaves, in addition to plant materials at different decomposition stages. Several species are found in the genus Leucothyreus, occurring in different regions of Brazil, including the various biomes in the country. Information on the biology of species of the genus Leucothyreus is scarce, therefore, we conducted studies on the biological aspects of Leucothyreus ambrosius Blanchard, 1850. The period of adult occurrence was determined with a light trap installed between a cropped and pasture area in the municipality of Aquidauana, Mato Grosso do Sul State, Brazil. Adults collected in the field were used to form insect pairs and the studies were initiated in the entomology laboratory as the adults began ovipositing. Adults were observed flying in the field from October to December. Eggs were obtained as pairs were formed and a colony was established, the embryonic period lasting 14.6 days on average. The larval period in the 1st instar lasted 21.6 days, in the 2nd instar 19.6 days, and in the 3rd instar, 85.6 days. The head capsule width was 1.48 mm in the 1st instar, 2.44 mm in the 2nd, and 3.83 mm in 3rd larval instar. The pupal stage had an average duration of 35.5 days. The egg to adult period lasted 173.3 days. Morphometric information for the larval and adult stages is presented in this study.

  14. Using malaise traps to sample ground beetles (Coleoptera. Carabidae)

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Hanula, James L. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Horn, Scott [USDA Forest Service, Savannah River, New Ellenton, SC (United States)

    2012-04-02

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  15. Biocontrol of larval mosquitoes by Acilius sulcatus (Coleoptera: Dytiscidae

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    Banerjee Siddhartha S

    2008-10-01

    Full Text Available Abstract Background Problems associated with resistant mosquitoes and the effects on non-target species by chemicals, evoke a reason to find alternative methods to control mosquitoes, like the use of natural predators. In this regard, aquatic coleopterans have been explored less compared to other insect predators. In the present study, an evaluation of the role of the larvae of Acilius sulcatus Linnaeus 1758 (Coleoptera: Dytiscidae as predator of mosquito immatures was made in the laboratory. Its efficacy under field condition was also determined to emphasize its potential as bio-control agent of mosquitoes. Methods In the laboratory, the predation potential of the larvae of A. sulcatus was assessed using the larvae of Culex quinquefasciatus Say 1823 (Diptera: Culicidae as prey at varying predator and prey densities and available space. Under field conditions, the effectiveness of the larvae of A. sulcatus was evaluated through augmentative release in ten cemented tanks hosting immatures of different mosquito species at varying density. The dip density changes in the mosquito immatures were used as indicator for the effectiveness of A. sulcatus larvae. Results A single larva of A. sulcatus consumed on an average 34 IV instar larvae of Cx. quinquefasciatus in a 24 h period. It was observed that feeding rate of A. sulcatus did not differ between the light-on (6 a.m. – 6 p.m., and dark (6 p.m. – 6 a.m. phases, but decreased with the volume of water i.e., space availability. The prey consumption of the larvae of A. sulcatus differed significantly (P A. sulcatus larvae, while with the withdrawal, a significant increase (p A. sulcatus in regulating mosquito immatures. In the control tanks, mean larval density did not differ (p > 0.05 throughout the study period. Conclusion the larvae of the dytiscid beetle A. sulcatus proved to be an efficient predator of mosquito immatures and may be useful in biocontrol of medically important mosquitoes.

  16. Interspecific Competition between Tribolium castaneum and Cryptolestes ferrugineus%赤拟谷盗Tribolium castaneum与锈赤扁谷盗Cryptolestes ferrugineus种间竞争的研究

    Institute of Scientific and Technical Information of China (English)

    周蕊; 邓永学; 王进军; 崔晋波; 李平

    2012-01-01

    Interspecific competition between Tribolium castaneum and Cryptolestes femigineus was studied at 30℃ ,75 % RH and Votka-Volt-erra equation was used to simulate the competition. The results showed that population numbers of T. castaneum and C. ferrugineus under single rearing condition were more than that under mixture rearing condition. The interspecific competition( resource competition type) between the two species was remarkable. Population number of T. castaneum under single rearing and mixture rearing condition was greater than that of C. femigineus. The two species could coexist steadily under mixture rearing condition and T. castaneum was dominant species in the competition system.%在30℃、75% RH条件下研究赤拟谷盗Tribolium castaneum与锈赤扁谷盗Crptolestes ferrugineus的种间竞争,并采用Votka-Volterra种间竞争模型进行拟合.结果表明,赤拟谷盗与锈赤腐谷盗混合饲养时的种群数量均小于单独饲养时的种群数量,两物种之间存在着显著的种间竞争关系.属于资源利用型竞争;单独饲养和混合饲养条件下赤拟谷盗的种群数量均大于锈赤扁谷盗的种群数量;混合饲养条件下两个物种可以共存,并形成稳定的平衡状态,在这个竞争系统中,赤拟谷盗为优势种.

  17. Gold bugs and beyond: a review of iridescence and structural colour mechanisms in beetles (Coleoptera).

    Science.gov (United States)

    Seago, Ainsley E; Brady, Parrish; Vigneron, Jean-Pol; Schultz, Tom D

    2009-04-01

    Members of the order Coleoptera are sometimes referred to as 'living jewels', in allusion to the strikingly diverse array of iridescence mechanisms and optical effects that have arisen in beetles. A number of novel and sophisticated reflectance mechanisms have been discovered in recent years, including three-dimensional photonic crystals and quasi-ordered coherent scattering arrays. However, the literature on beetle structural coloration is often redundant and lacks synthesis, with little interchange between the entomological and optical research communities. Here, an overview is provided for all iridescence mechanisms observed in Coleoptera. Types of iridescence are illustrated and classified into three mechanistic groups: multilayer reflectors, three-dimensional photonic crystals and diffraction gratings. Taxonomic and phylogenetic distributions are provided, along with discussion of the putative functions and evolutionary pathways by which iridescence has repeatedly arisen in beetles.

  18. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Dilma Solange Napp

    2006-01-01

    Full Text Available Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae. Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae. The following new genus and species, all from Bolivia, are described in Compsocerini: Ecoporanga gen. nov., type species E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., from Santa Cruz, and Ethemon iuba sp. nov. from Cochabamba.

  19. Una especie nueva de Trechisibus de la Argentina (Coleoptera: Carabidae A new species of Trechisibus from Argentina (Coleoptera: Carabidae

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    Sergio Roig-Juñent

    2005-12-01

    Full Text Available RESUMEN. Las Trechini constituyen una tribu de Carabidae distribuida ampliamente en la región Andino Patagónica. A pesar de que existen varias revisiones parciales de esta tribu para la Argentina y Chile, es muy común hallar especies nuevas, debido sobre todo a la prospección de áreas no exploradas. En este aporte se describe una especie nueva del género Trechisibus Jeannel del Cerro Nevado (Mendoza, Argentina. Por sus características morfológicas pertenece al grupo de especies depressus. Se describe e ilustra el adulto de esta especie nueva, se provee una clave para la identificación de las especies del grupo depressus y se discuten algunos aspectos de la distribución de estas especies.ABSTRACT. Trechini is a tribe of Carabidae (Coleoptera widely distributed in the Andean Patagonian region in South America. In spite of several partial revisions of the tribe for Argentina and Chile, it is very common to find new species due to the research of unexplored areas. In the present paper, a new species of the genus Trechisibus Jeannel from the Cerro Nevado (Mendoza, Argentina is described. Based on its morphological features the new species is considered as a member of the depressus group. The adult of the new species is described and illustrated, a key for the identification of the species of the depressus group is provided, and some aspects of the distribution of the group are discussed.

  20. Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil

    OpenAIRE

    Renata C. Campos; Malva I. Medina Hernández

    2013-01-01

    Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil. The beetles of the subfamily Scarabaeinae are important organisms that participate in the cycle of decomposition, especially in tropical ecosystems. Most species feed on feces (dung) or carcasses (carrion) and are associated with animals that produce their food resources. Dung beetles are divided into three functional groups: rollers, tunnelers and dwellers. This present work aims to study the ...

  1. The Leaf-Beetles (Coleoptera, Chrysomelidae) Feed On Some Weeds In Tokat Province

    OpenAIRE

    Çam, Halit; ATAY, Turgut

    2004-01-01

    This study was carried out to determine the leaf-beetles (Coleoptera, Chrysomelidae) which causes extensive damage on weeds in the vicinity of Tokat, Turkey. Total 9 taxa including 4 species of Chrysomelinae, 1 species of Clytrinae, 1 species of Criocerinae, 2 species of Alticinae and 1 species of Cassidinae were found to be destructive on different weed species. These species were; Entomoscelis adonidis (Pall.) on Sinapis arvensis L., Gastrophysa polygoni (L.) on Polygonum convolvulus L. an...

  2. Species of beetles (Coleoptera; Scarabaeidae associated to banana (Musa spp. in Ceballos, Ciego de Avila, Cuba

    Directory of Open Access Journals (Sweden)

    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in bananas plantations, according to Sisne, 2009 and MINAG, 1985, in the Citric enterprise of Ciego de Ávila during the period between May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species Cyclocephala cubana Chapin, Phyllophaga puberula Duval, and Phyllophaga patruelis Chev. are associated to bananas crops in these areas.

  3. Ecological impact of entomopathogenic nematodes used to control the large pine weevil, Hylobius abietis (Coleoptera: Curculionidae)

    OpenAIRE

    Harvey, Christopher D.

    2010-01-01

    The large pine weevil (Hylobius abietis; Coleoptera: Curculionidae) is one of the most economically damaging pests in forestry across Northern Europe. Its larvae develop in coniferous tree stumps on clearfell sites and adult weevils feed on seedlings that are replanted on these sites, causing substantial mortality. As the drive towards achieving the sustainable management of natural resources increases, biological control agents are being considered as an alternative to chemical ...

  4. Otiorhynchus spp. (Coleoptera: Curculionidae) as pests in horticulture: genetics and management options with entomopathogenic fungi

    OpenAIRE

    Hirsch, Jacqueline

    2012-01-01

    Worldwide, weevils of the genus Otiorhynchus (Coleoptera: Curculionidae) cause damage with detrimental economic effects to many horticultural crops due to the root feeding of their larvae as well as foliage feeding of their adults. Aside from the black vine weevil Otiorhynchus sulcatus, which is the best-known pest within this genus, numerous other Otiorhynchus species have been increasingly recognized as pests in recent years. Nocturnal adult weevils and soil-inhibiting larvae are in princip...

  5. Laboratory Rearing of Laricobius nigrinus (Coleoptera: Derodontidae): A Predator of the Hemlock Woolly Adelgid (Hemiptera: Adelgidae)

    OpenAIRE

    Salom, S. M.; L. T. Kok; Lamb, A B; Jubb, C

    2012-01-01

    Coleopteran species are biological control agents of numerous invasive pests. Laricobius nigrinus (Coleoptera: Derodontidae), a predaceous, univoltine species, spends the summer aestivating but is active for the rest of the year. Laricobius nigrinus possesses many essential attributes for effective biological control of the hemlock woolly adelgid (Hemiptera: Adelgidae). The predator must be reared in large numbers for field releases. We describe some of the studies that led to the successful ...

  6. New national and state records of Neotropical Staphylinidae (Insecta: Coleoptera).

    Science.gov (United States)

    Asiain, Julieta; Márquez, Juan; Irmler, Urlich

    2015-01-01

    Eighteen new national records of nine species of Osoriinae (Staphylinidae) are added for 10 Neotropical countries. Additionally, 17 species of three subfamilies are first recorded from ten States of México. The distributional patterns of the studied species are commented and the congruence with species of different families of Coleoptera and Odonata previously analyzed is discussed. Finally, we conclude that some of these patterns can be proposed as hypothesis of primary biogeographic homology. PMID:26249885

  7. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern

    Directory of Open Access Journals (Sweden)

    SUGIYARTO

    2009-07-01

    Full Text Available Maryati KT, Sugiyarto. 2010. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern. Nusantara Bioscience 1: 72-77. This research aims to find out the white grub (Melolonthidae; Coleoptera variability based on the morphological characteristic and protein banding pattern found in ”salak pondoh” farm in Regencies of Sleman, Yogyakarta and Magelang, Central Java. Each area has five sampling points. Morphological analysis on white grub was conducted using descriptive method and analysis on protein banding pattern was conducted using qualitative analysis based on the presence or absent of band pattern on the gel, and qualitatively based on the relative mobility value (Rf of protein. The result indicated that the white grub in Sleman and Magelang, based on morphology characteristic is only one species, namely Holothricia sp. Based on the protein banding pattern, the white grub sample have differences of protein band number and protein molecular weight. Key words: Salacca zalacca, white grub, morphology, protein banding pattern.Abstrak. Maryati KT, Sugiyarto. 2010. Karakterisasi lundi putih (Melolonthidae: Coleoptera pada pertanaman salak berdasarkan ciri morfologi dan pola pita protein. Nusantara Bioscience 1: 72-77. Penelitian ini bertujuan untuk mengetahui keanekaragaman lundi putih (Melolonthidae; Coleoptera berdasarkan ciri morfologi dan pola pita protein yang ditemukan di lahan pertanaman salak pondoh di Kabupaten Sleman, Yogyakarta dan Kabupaten Magelang, Jawa Tengah. Pada masing-masing wilayah diambil lima titik sampling. Analisis morfologi lundi putih digunakan metode deskriptif, dan analisis pola pita protein digunakan analisis kualitatif berdasarkan muncul tidaknya pola pita pada gel, dan secara kuantitatif berdasarkan nilai mobilitas relatif protein (RF. Hasil penelitian menunjukkan bahwa sampel lundi putih di Kabupaten Sleman dan Magelang, berdasar karakter

  8. Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil

    OpenAIRE

    Andreia Mauruto Chernaki-Leffer; Daniel Ricardo Sosa-Gómez; Lúcia M. de Almeida; Ivani de Oliveira Negrão Lopes

    2011-01-01

    Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer), is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvo...

  9. Novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae da Bolívia

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    Ubirajara R. Martins

    2008-01-01

    Full Text Available Novas espécies descritas da Bolívia, Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.New species of Onciderini (Coleoptera, Cerambycidae, Lamiinae from Bolivia. New species described from Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.

  10. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae)

    OpenAIRE

    M.M. Kiradoo; Meera Srivastava

    2011-01-01

    The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae) is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insect...

  11. NEW CONTRIBUTION CONCERNING THE MASS REARING OF TANYMECUS DILATICOLLIS GYLL (COLEOPTERA: CURCULIONIDAE) UNDER CONTROLLED CONDITIONS

    OpenAIRE

    MANOLE Traian; Irina IONESCU-MĂLĂNCUŞ; Iuliana ANTONIE; Laurenţiu Constantin REBEGA

    2013-01-01

    Controlled growth in the laboratory of some species of insect’s are justified both scientifically and practically. Due to the economic importance that the corn crop pest Tanymecus dilaticollis Gyll. (Coleoptera: Curculionidae) had in Romania like the main pest of maize crops we tried to obtain successive generations under environment controlled of the insect mentioned. In this paper we aim to improve the efficiency of the multiplication of the species with respect to two aspects: attending la...

  12. DIVERSITY OF CARABIDS (COLEOPTERA) ASSOCIATED WITH THE LOWER LURIN RIVER, LIMA, PERU

    OpenAIRE

    ARMANDO VÉLEZ-AZAÑERO; Alfonso Lizárraga-Travaglini

    2013-01-01

    Quarterly samples of beetles (Coleoptera: Carabidae) were taken associated with the lower basin of the Lurín River (Lima-Peru) during the period August 2009 - February 2011, in six sampling points between 5 and 51 masl. Pitfall traps were used and obtained a total of 59 specimens distributed among three tribes, three genera, and four morphospecies. We report the presence of Megacephala, Scarites genus, and Pterostichus with the latter being the predominant genus.

  13. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae) in Cotton

    OpenAIRE

    P. G. Tillman; Cottrell, T. E.

    2012-01-01

    Lady beetles (Coleoptera: Coccinellidae) prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L.), Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas), Coleomegilla maculata (De Geer), Cycloneda munda (Say), and Olla v-nigrum (Mulsant) were found in sorghum over both years. Lady beetle compositions in sorghum and ...

  14. Biological activities of Allium sativum essential oil against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae)

    OpenAIRE

    Chaubey Mukesh Kumar

    2014-01-01

    Essential oil from Allium sativum was isolated and investigated for its repellent, insecticidal, ovipositional and egg hatching inhibition activities against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). A. sativum essential oil repelled bruchid adults at a very low concentration in choice oviposition assay. A. sativum essential oil caused both fumigant and contact toxicity in C. chinensis adults in a concentration dependent manner. Oviposition potency of C. chinensis adults...

  15. Control Effectiveness of Insect Growth Regulators on Tribolium castaneum%两种昆虫生长调节剂对赤拟谷盗的控制效果

    Institute of Scientific and Technical Information of China (English)

    陈雅群; 谢令德; 张建珍; 贺艳萍; 何君; 陈春武

    2012-01-01

    选取氟啶脲( Chlorfluazuron)和灭幼宝(Pyriproxyfen)两种昆虫生长调节剂对储藏物害虫赤拟谷盗进行了控制效果的研究.结果表明,在40、20、10 mg/kg剂量下,氟啶脲对赤拟谷盗幼虫有较高的校正死亡率,依次为94.44%、88.89%、83.33%,幼虫均不能化蛹,表明该药剂可能作用于几丁质合成酶,抑制了几丁质合成酶的活性.在相同浓度下,灭幼宝对赤拟谷盗幼虫没有明显的致死效果,但其能延长赤拟谷盗幼虫的发育历期,使其不能正常化蛹,最终导致畸形死亡,表明灭幼宝有类似保幼激素的作用.%The paper study the control effectiveness of two IGRs, Chlorfluazuron and Pyriproxyfen to Tribolium castaneum, a main insect pest occurring in stored product. The result showed that Chlorfluazuron had a high mortality rate on Tribolium castaneum larva, which is 94.44% ,,88. 89% ,,83. 33% corresponding to the concentration of 40,20, 10 mg/kg. ,and make them pupate hardly,so Chlorfluazuron had significant death effect on Tribolium castaneum larva , and it acted on chitin synthase, inhibiting the activity of chitin synthase. Pyriproxyfen didn't have obvious death effect on Tribolium castaneum at the same concentration as Chlorfluazuron, but Pyriproxyfen can delay the development of Tribolium castaneum larva, affect their normal pupa, and eventually cause their deformity death. The result preliminary shows that Pyriproxyfen has similar function as juvenile hormones.

  16. Alien seed beetles (Coleoptera: Chrysomelidae: Bruchinae) in Europe.

    Science.gov (United States)

    Yus-Ramos, Rafael; Ventura, Daniel; Bensusan, Keith; Coello-García, Pedro; György, Zoltán; Stojanova, Anelia

    2014-07-01

    Under the framework of the DAISIE consortium, whose main mission is to make an inventory of the alien invasive species of Europe and its islands, we review the current state of knowledge and provide an up-to-date catalogue and distributional status for alien seed beetles (Coleoptera: Chrysomelidae: Bruchinae) in Europe. This work is based on studies of the species detected from the last century to the present, but with greater emphasis on the beginning of the 21st century, during which new biological studies have been carried out and findings made in European countries. The main objective of this paper is to focus on this last fact, which has promoted new views on the existing and potential threat of exotic bruchids in relation to climate change. This must now be regarded as a matter of concern for European agricultural and environmental policies. Only species of exotic origin introduced in European regions outside their native range were considered. Therefore, species of European origin spreading to new countries within Europe are not treated. Also, we provide a new approach to classifying alien seed beetle species according to their ability to become established, distinguishing between the well-established and those that may appear in seed stores but are not capable of invading natural and agricultural ecosystems. We present a taxonomic characterization of the alien bruchids found in Europe, providing an illustrated key based on external morphological characters of adults. The key facilitates the identification of the sixteen most frequently recorded genera, which represent 37 of the 42 species of exotic species recorded in Europe up to the present, whether established, not established or occasional. Finally, we provide a summary of the state of knowledge of the taxonomy and biology of the 20 most worrying species as pests, both established and non-established. This includes, where appropriate, an illustrated key for the identification of species. The study

  17. Identification of G protein-coupled receptors required for vitellogenin uptake into the oocytes of the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Bai, Hua; Palli, Subba Reddy

    2016-01-01

    Previous studies suggested that a membrane receptor might be involved in mediating vitellogenin (Vg) uptake and juvenile hormone (JH)-regulated remodeling of follicular epithelium (also called 'patency'). G protein-coupled receptor (GPCR) family is one of the largest membrane receptor protein families and controls many key physiological processes. To investigate the role of GPCRs in insect reproduction and juvenile hormone-regulated Vg uptake, we performed a comprehensive RNA interference (RNAi) screen targeting GPCRs in the red flour beetle, Tribolium castaneum. Out of 112 GPCRs tested, knockdown of 41 GPCRs resulted in a reduction in fecundity. Interestingly, RNAi against two GPCRs (a Rhodopsin-like receptor and a Dopamine D2-like receptor) led to a significant reduction in Vg accumulation in developing oocytes. Functional assays of these two GPCRs showed that JH triggers a dose-dependent inhibition of intracellular cAMP levels in HEK293 cells expressing Tribolium Dopamine D2-like receptor. These data suggest that Dopamine D2-like receptor plays crucial roles in regulating Vg uptake and is a promising candidate membrane receptor mediating JH regulation of patency in the red flour beetle. PMID:27277501

  18. The negative effect of starvation and the positive effect of mild thermal stress on thermal tolerance of the red flour beetle, Tribolium castaneum

    Science.gov (United States)

    Scharf, Inon; Wexler, Yonatan; MacMillan, Heath Andrew; Presman, Shira; Simson, Eddie; Rosenstein, Shai

    2016-04-01

    The thermal tolerance of a terrestrial insect species can vary as a result of differences in population origin, developmental stage, age, and sex, as well as via phenotypic plasticity induced in response to changes in the abiotic environment. Here, we studied the effects of both starvation and mild cold and heat shocks on the thermal tolerance of the red flour beetle, Tribolium castaneum. Starvation led to impaired cold tolerance, measured as chill coma recovery time, and this effect, which was stronger in males than females, persisted for longer than 2 days but less than 7 days. Heat tolerance, measured as heat knockdown time, was not affected by starvation. Our results highlight the difficulty faced by insects when encountering multiple stressors simultaneously and indicate physiological trade-offs. Both mild cold and heat shocks led to improved heat tolerance in both sexes. It could be that both mild shocks lead to the expression of heat shock proteins, enhancing heat tolerance in the short run. Cold tolerance was not affected by previous mild cold shock, suggesting that such a cold shock, as a single event, causes little stress and hence elicits only weak physiological reaction. However, previous mild heat stress led to improved cold tolerance but only in males. Our results point to both hardening and cross-tolerance between cold and heat shocks.

  19. Infection of Tribolium castaneum with Bacillus thuringiensis: quantification of bacterial replication within cadavers, transmission via cannibalism, and inhibition of spore germination.

    Science.gov (United States)

    Milutinović, Barbara; Höfling, Christina; Futo, Momir; Scharsack, Jörn P; Kurtz, Joachim

    2015-12-01

    Reproduction within a host and transmission to the next host are crucial for the virulence and fitness of pathogens. Nevertheless, basic knowledge about such parameters is often missing from the literature, even for well-studied bacteria, such as Bacillus thuringiensis, an endospore-forming insect pathogen, which infects its hosts via the oral route. To characterize bacterial replication success, we made use of an experimental oral infection system for the red flour beetle Tribolium castaneum and developed a flow cytometric assay for the quantification of both spore ingestion by the individual beetle larvae and the resulting spore load after bacterial replication and resporulation within cadavers. On average, spore numbers increased 460-fold, showing that Bacillus thuringiensis grows and replicates successfully in insect cadavers. By inoculating cadaver-derived spores and spores from bacterial stock cultures into nutrient medium, we next investigated outgrowth characteristics of vegetative cells and found that cadaver-derived bacteria showed reduced growth compared to bacteria from the stock cultures. Interestingly, this reduced growth was a consequence of inhibited spore germination, probably originating from the host and resulting in reduced host mortality in subsequent infections by cadaver-derived spores. Nevertheless, we further showed that Bacillus thuringiensis transmission was possible via larval cannibalism when no other food was offered. These results contribute to our understanding of the ecology of Bacillus thuringiensis as an insect pathogen. PMID:26386058

  20. Response of Tribolium castaneum to elevated copper concentrations is influenced by history of metal exposure, sex-specific defences, and infection by the parasite Steinernema feltiae.

    Science.gov (United States)

    Kramarz, Paulina E; Mordarska, Anna; Mroczka, Magdalena

    2014-07-01

    We studied how copper toxicity in the red flour beetle, Tribolium castaneum changed as a result of infection by the entomopathogenic nematode Steinernema feltiae. Measured traits were: respiration, growth and survival, as well as the concentrations of copper within beetle tissues and in its diet. By comparing F1 and F5 generation we were able to answer how long-term metal exposure changed the responses to both copper and the parasite. The beetles did accumulate copper; however, the results indicated that copper concentrations in beetle tissues were affected by nematode infection, the sex of the experimental animals, and the number of generations of exposure. Five generations of exposure to copper resulted in the highest dry body mass of infected beetles of both sexes; additionally, this group also had the lowest copper concentrations in their tissues. The only factor that had a significant effect on respiration was infection by nematodes: infected beetles of both sexes in both generational groups had significantly decreased respiration rates. Survival was lowest in nematode-infected animals of both sexes from both generations, regardless of exposure to copper. Our results confirm that an organism's response to metal pollution is dependent on its health status and sex. We also found that the history of exposure to metal was equally important-we found enhanced resistance to copper intoxication after only five generations of exposure.

  1. The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference

    Directory of Open Access Journals (Sweden)

    Da Xiao

    2014-04-01

    Full Text Available We identified and characterized the TcLgl gene putatively encoding lethal giant larvae (Lgl protein from the red flour beetle (Tribolium castaneum. Analyses of developmental stage and tissue-specific expression patterns revealed that TcLgl was constitutively expressed. To examine the role of TcLgl in insect development, RNA interference was performed in early (1-day larvae, late (20-day larvae, and early (1-day pupae. The early larvae injected with double-stranded RNA of TcLgl (dsTcLgl at 100, 200, and 400 ng/larva failed to pupate, and 100% mortality was achieved within 20 days after the injection or before the pupation. The late larvae injected with dsTcLgl at these doses reduced the pupation rates to only 50.3%, 36.0%, and 18.2%, respectively. The un-pupated larvae gradually died after one week, and visually unaffected pupae failed to emerge into adults and died during the pupal stage. Similarly, when early pupae were injected with dsTcLgl at these doses, the normal eclosion rates were reduced to only 22.5%, 18.0%, and 11.2%, respectively, on day 7 after the injection, and all the adults with abnormal eclosion died in two days after the eclosion. These results indicate that TcLgl plays an essential role in insect development, especially during their metamorphosis.

  2. Genome-wide analysis of tandem repeats in Tribolium castaneum genome reveals abundant and highly dynamic tandem repeat families with satellite DNA features in euchromatic chromosomal arms.

    Science.gov (United States)

    Pavlek, Martina; Gelfand, Yevgeniy; Plohl, Miroslav; Meštrović, Nevenka

    2015-12-01

    Although satellite DNAs are well-explored components of heterochromatin and centromeres, little is known about emergence, dispersal and possible impact of comparably structured tandem repeats (TRs) on the genome-wide scale. Our bioinformatics analysis of assembled Tribolium castaneum genome disclosed significant contribution of TRs in euchromatic chromosomal arms and clear predominance of satellite DNA-typical 170 bp monomers in arrays of ≥5 repeats. By applying different experimental approaches, we revealed that the nine most prominent TR families Cast1-Cast9 extracted from the assembly comprise ∼4.3% of the entire genome and reside almost exclusively in euchromatic regions. Among them, seven families that build ∼3.9% of the genome are based on ∼170 and ∼340 bp long monomers. Results of phylogenetic analyses of 2500 monomers originating from these families show high-sequence dynamics, evident by extensive exchanges between arrays on non-homologous chromosomes. In addition, our analysis shows that concerted evolution acts more efficiently on longer than on shorter arrays. Efficient genome-wide distribution of nine TR families implies the role of transposition only in expansion of the most dispersed family, and involvement of other mechanisms is anticipated. Despite similarities in sequence features, FISH experiments indicate high-level compartmentalization of centromeric and euchromatic tandem repeats.

  3. Infection of Tribolium castaneum with Bacillus thuringiensis: quantification of bacterial replication within cadavers, transmission via cannibalism, and inhibition of spore germination.

    Science.gov (United States)

    Milutinović, Barbara; Höfling, Christina; Futo, Momir; Scharsack, Jörn P; Kurtz, Joachim

    2015-12-01

    Reproduction within a host and transmission to the next host are crucial for the virulence and fitness of pathogens. Nevertheless, basic knowledge about such parameters is often missing from the literature, even for well-studied bacteria, such as Bacillus thuringiensis, an endospore-forming insect pathogen, which infects its hosts via the oral route. To characterize bacterial replication success, we made use of an experimental oral infection system for the red flour beetle Tribolium castaneum and developed a flow cytometric assay for the quantification of both spore ingestion by the individual beetle larvae and the resulting spore load after bacterial replication and resporulation within cadavers. On average, spore numbers increased 460-fold, showing that Bacillus thuringiensis grows and replicates successfully in insect cadavers. By inoculating cadaver-derived spores and spores from bacterial stock cultures into nutrient medium, we next investigated outgrowth characteristics of vegetative cells and found that cadaver-derived bacteria showed reduced growth compared to bacteria from the stock cultures. Interestingly, this reduced growth was a consequence of inhibited spore germination, probably originating from the host and resulting in reduced host mortality in subsequent infections by cadaver-derived spores. Nevertheless, we further showed that Bacillus thuringiensis transmission was possible via larval cannibalism when no other food was offered. These results contribute to our understanding of the ecology of Bacillus thuringiensis as an insect pathogen.

  4. The negative effect of starvation and the positive effect of mild thermal stress on thermal tolerance of the red flour beetle, Tribolium castaneum.

    Science.gov (United States)

    Scharf, Inon; Wexler, Yonatan; MacMillan, Heath Andrew; Presman, Shira; Simson, Eddie; Rosenstein, Shai

    2016-04-01

    The thermal tolerance of a terrestrial insect species can vary as a result of differences in population origin, developmental stage, age, and sex, as well as via phenotypic plasticity induced in response to changes in the abiotic environment. Here, we studied the effects of both starvation and mild cold and heat shocks on the thermal tolerance of the red flour beetle, Tribolium castaneum. Starvation led to impaired cold tolerance, measured as chill coma recovery time, and this effect, which was stronger in males than females, persisted for longer than 2 days but less than 7 days. Heat tolerance, measured as heat knockdown time, was not affected by starvation. Our results highlight the difficulty faced by insects when encountering multiple stressors simultaneously and indicate physiological trade-offs. Both mild cold and heat shocks led to improved heat tolerance in both sexes. It could be that both mild shocks lead to the expression of heat shock proteins, enhancing heat tolerance in the short run. Cold tolerance was not affected by previous mild cold shock, suggesting that such a cold shock, as a single event, causes little stress and hence elicits only weak physiological reaction. However, previous mild heat stress led to improved cold tolerance but only in males. Our results point to both hardening and cross-tolerance between cold and heat shocks.

  5. Properties and natural occurrence of maternal-effect selfish genes ('Medea' factors) in the red flour beetle, Tribolium castaneum

    International Nuclear Information System (INIS)

    Maternally acting selfish genes, termed 'Medea' factors, were found to be widespread in wild populations of Tribolium castaneum collected in Europe, North and South America, Africa and south-east Asia, but were rare or absent in populations from Australia and the Indian subcontinent. We detected at least four distinct genetic loci in at least two different linkage groups that exhibit the Medea pattern of differential mortality of genotypes within maternal families. Although each M factor tested had similar properties of maternal lethality to larvae and zygotic self-rescue, M factors representing distinct loci did not show cross-rescue. Alleles at two of these loci, M1 and M4, were by far the most prevalent, M4 being the predominant type. M2 and M3 were each found only once, in Pakistan and Japan, respectively. Although M1 could be genetically segregated from M4 and maintained as a purified stock, the M1 factor invariably co-occurred with M4 in field populations, whereas M4 usually occurred in the absence of other Medea factors. The dominant maternal lethal action of M1 could be selectively inactivated (reverted) by gene-knockout gamma irradiation with retention of zygotic rescue activity. (author)

  6. Laboratory Bioassay of Iranian Isolates of Entomopathogenic Fungus Metarhizium anisopliae (Metsch. Sorokin (Ascomycota: Hypocreales against two Species of Storage Pest

    Directory of Open Access Journals (Sweden)

    Adel Khashaveh

    2013-03-01

    Full Text Available M-B 12.00 Normal 0 false false false EN-US X-NONE FA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";} The susceptibility of adults of Tribolium castaneum Herbst (Coleoptera: Tenebrionidae and Oryzaephilus surinamensis L. (Coleoptera: Silvanidae to three Iranian Isolates of the entomopathogenic fungi Metarhizium anisopliae (Metsch. Sorokin was evaluated through bioassays with direct immersion under laboratory conditions. For each isolates, five aqueous suspensions were prepared in a logarithmic series in Tween 80 (0.05% v/v. Results showed that adult of both species were susceptible to all isolates of M. anisopliae. For all three isolates, mortality percentage of the two species increased with increasing conidial concentration and significant difference was observed between concentrations. The corrected cumulative mortality of adult of O. surinamensis 10 days after immersion ranged from 12.38 to 85.84%, 18.6 to 62.83% and 10.63 to 77.87% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. These amounts for T. castaneum varied from 31.07 to 74.78%, 26.02 to 75.61% and 23.33 to 89.99% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. The parameters of probit analysis demonstrated non-overlap of 95% confidence limits of LC50 and LC95 and significant difference was observed among three isolates tested against each insect. The lowest and the highest LC50 and LC95 values were observed in the isolates DEMI001 for O. surinamensis (3/1×105 and 1/5×108 and IRAN 715C for T. castaneum (6.2×108 and 6.9×1014, respectively. This

  7. Comparative Growth and Survival of Hylurgus ligniperda (Coleoptera: Scolytinae) and Arhopalus ferus (Coleoptera: Cerambycidae) Reared on Artificial or Natural Diet at 15 or 25°C.

    Science.gov (United States)

    Romo, C M; Bader, M K-F; Pawson, S M

    2016-02-01

    Two saproxylic forest insects, Hylurgus ligniperda (F.) (Coleoptera: Scolytinae) and Arhopalus ferus (Mulsant)(Coleoptera: Cerambycidae), were reared on artificial or natural diet at 15 or 25°C to compare larval growth rates and survival. A significant diet by temperature interaction was observed in the growth of H. ligniperda larvae,which developed faster when reared on natural diet at 15°C, but grew faster and pupated significantly earlier when reared on artificial diet at 25°C. However, H. ligniperda survival by the end of the experiment was low on both diets when reared at 25°C (10.1%, 95% CI: 5.2–15.1%), which suggests that rearing at lower temperatures may be required. A. ferus larvae gained significantly larger body size when reared on artificial diet than on natural diet at both temperatures. Survival of A. ferus reared on artificial diet was significantly lower than larvae reared on natural diet at 25°C. The significant differences between A. ferus larval development rates when reared on artificial and natural diets preclude the use of artificial diet to collect meaningful data to construct temperature development models for ecological comparisons. Artificial diet provided a suitable medium for mass production of individuals for research purposes, e.g., test mortality in response to treatments. However, additional rearing studies are needed to determine whether the larger artificially reared larvae result in adults that are healthier, more productive, and live longer.

  8. Especies mexicanas de Curculionidae (Insecta: Coleoptera asociadas con agaves (Asparagaceae: Agavoideae Mexican species of Curculionidae (Insecta: Coleoptera associated to agaves (Asparagaceae: Agavoideae

    Directory of Open Access Journals (Sweden)

    Alberto Romo

    2012-12-01

    Full Text Available Se estudiaron las especies de picudos o gorgojos (Coleoptera: Curculionidae asociadas con agaves (Asparagaceae: Agavoideae en México. Se registraron 5 especies asociadas con especies de Agave, Furcraea, Hesperoyucca, Polianthes y Yucca; de éstas, 4 pertenecen a la subfamilia Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis y Cactophagus spinolae y 1 a la Baridinae (Peltophorus polymitus. Se presentan diagnosis, ilustraciones y una clave para la identificación de las 5 especies de curculiónidos asociados con agaves. Se establecen las siguientes sinonimias: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; y Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891 y Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.Weevils (Coleoptera: Curculionidae associated with agaves (Asparagaceae: Agavoideae from Mexico are analyzed. Five species were recorded associated with species of Agave, Furcraea, Hesperoyucca, Polianthes, and Yucca. Four of these species belong to the subfamily Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis, and Cactophagus spinolae and 1 belongs to the subfamily Baridinae (Peltophorus polymitus. Diagnoses, illustrations and a key are presented for identifying the 5 species of weevils found on agaves. The following synonymies are established: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; and Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891, and Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.

  9. New myrmecomorphous longhorned beetles from Haiti and the Dominican Republic with a key to Anaglyptini and Tillomorphini of Hispaniola (Coleoptera: Cerambycidae: Cerambycinae)

    Science.gov (United States)

    First records of the tribes Anaglyptini and Tillomorphini (Coleoptera: Cerambycidae: Cerambycinae) are documented for Hispaniola. A new genus of highly myrmecomorphic longhorned beetle (Licracantha, new genus) is described and illustrated based on one species (Licracantha formicaria, new species) a...

  10. The influence of vegetation and landscape structural connectivity on butterflies (Lepidoptera: Papilionoidea and Hesperiidae), Carabids (Coleoptera: Carabidae), Syrphids (Diptera: Syrphidae), and sawflies (Hymenoptera: Symphyta) in Northern Italy farmland

    NARCIS (Netherlands)

    Burgio, G.; Sommaggio, D.; Marini, M.; Chiarucci, A.; Landi, S.; Fabbri, R.; Pesarini, F.; Genghini, M.; Ferrari, R.; Muzzi, E.; Lenteren, van J.C.; Masetti, A.

    2015-01-01

    Landscape structure as well as local vegetation influence biodiversity in agroecosystems. A study was performed to evaluate the effect of floristic diversity, vegetation patterns, and landscape structural connectivity on butterflies (Lepidoptera: Papilionoidea and Hesperiidae), carabids (Coleoptera:

  11. Metamorphic labral axis patterning in the beetle Tribolium castaneum requires multiple upstream, but few downstream, genes in the appendage patterning network.

    Science.gov (United States)

    Smith, Frank W; Angelini, David R; Gaudio, Matthew S; Jockusch, Elizabeth L

    2014-03-01

    The arthropod labrum is an anterior appendage-like structure that forms the dorsal side of the preoral cavity. Conflicting interpretations of fossil, nervous system, and developmental data have led to a proliferation of scenarios for labral evolution. The best supported hypothesis is that the labrum is a novel structure that shares development with appendages as a result of co-option. Here, we use RNA interference in the red flour beetle Tribolium castaneum to compare metamorphic patterning of the labrum to previously published data on ventral appendage patterning. As expected under the co-option hypothesis, depletion of several genes resulted in similar defects in the labrum and ventral appendages. These include proximal deletions and proximal-to-distal transformations resulting from depletion of the leg gap genes homothorax and extradenticle, large-scale deletions resulting from depletion of the leg gap gene Distal-less, and smaller distal deletions resulting from knockdown of the EGF ligand Keren. However, depletion of dachshund and many of the genes that function downstream of the leg gap genes in the ventral appendages had either subtle or no effects on labral axis patterning. This pattern of partial similarity suggests that upstream genes act through different downstream targets in the labrum. We also discovered that many appendage axis patterning genes have roles in patterning the epipharyngeal sensillum array, suggesting that they have become integrated into a novel regulatory network. These genes include Notch, Delta, and decapentaplegic, and the transcription factors abrupt, bric à brac, homothorax, extradenticle and the paralogs apterous a and apterous b.

  12. Population growth rate and genetic variability of small and large populations of Red flour beetle (Tribolium castaneum) following multigenerational exposure to copper.

    Science.gov (United States)

    Laskowski, Ryszard; Radwan, Jacek; Kuduk, Katarzyna; Mendrok, Magdalena; Kramarz, Paulina

    2015-07-01

    We reared large (1000 individuals) and small (20 individuals) populations of Tribolium castaneum on diet contaminated with copper in order to determine if the size of a population affects its ability to adapt to adverse environmental conditions. After 10 generations, we used microsatellite markers to estimate and subsequently compare the genetic variability of the copper-treated populations with that of the control populations, which were reared on uncontaminated medium. Additionally, we conducted a full cross-factorial experiment which evaluated the effects of 10 generations of "pre-exposure" to copper on a population's fitness in control and copper-contaminated environments. In order to distinguish results potentially arising from genetic adaptation from those due to non-genetic effects associated to parental exposure to copper, we subjected also F11 generation, originating from parents not exposed to copper, to the same cross-factorial experiment. The effects of long-term exposure to copper depended on population size: the growth rates of small populations that were pre-exposed to copper were inhibited compared to those of small populations reared in uncontaminated environments. Large Cu-exposed populations had a higher growth rate in the F10 generation compared to the control groups, while the growth rate of the F11 generation was unaffected by copper exposure history. The only factor that had a significant effect on genetic variability was population size, but this was to be expected given the large difference in the number of individuals between large and small populations. Neither copper contamination nor its interaction with population size affected the number of microsatellite alleles retained in the F10 generation.

  13. Unexpected functional diversity in the fatty acid desaturases of the flour beetle Tribolium castaneum and identification of key residues determining activity.

    Science.gov (United States)

    Haritos, Victoria S; Horne, Irene; Damcevski, Katherine; Glover, Karen; Gibb, Nerida

    2014-08-01

    Desaturases catalyse modifications to fatty acids which are essential to homeostasis and for pheromone and defensive chemical production. All desaturases of the flour beetle Tribolium castaneum were investigated via query of the sequenced genome which yielded 15 putative acyl-Coenzyme A genes. Eleven desaturase mRNA were obtained in full length and functionally expressed in yeast. Phylogenetic analysis separated the desaturases into 4 distinct clades; one clade contained conserved beetle Δ9 desaturases, second clade was Tribolium-specific having diverse activities including Δ5, Δ9 and Δ12 desaturation and the other 2 clades had mixed insect representatives. Three members of this clade contained unusual inserted sequences of ∼20 residues in the C-terminal region and were related to desaturases that all contained similar inserts. Deletion of the entirety of the insert in the flour beetle Δ12 desaturase abolished its activity but this was partially restored by the reintroduction of two histidine residues, suggesting the histidine(s) are required for activity but the full length insert is not. Five new desaturase activities were discovered: Δ9 desaturation of C12:0-C16:0 substrates; two unprecedented Δ5 enzymes acting on C18:0 and C16:0; Δ9 activity exclusively on C16:0 and a further stearate Δ9 desaturase. qPCR analysis ruled out a role in sex pheromone synthesis for the Δ5 and Δ9/C16:0 desaturases. The flour beetle genome has underpinned an examination of all transcribed desaturases in the organism and revealed a diversity of novel and unusual activities, an improved understanding of the evolutionary relationships among insect desaturases and sequence determinants of activity.

  14. Nuevos datos de distribución de los Cholevinae hipogeos del Atlas marroquí (Coleoptera, Leiodidae

    Directory of Open Access Journals (Sweden)

    Fresneda, J.

    2006-12-01

    Full Text Available New distribution data for the hypogean Cholevinae from the Moroccan Atlas (Coleoptera, Leiodidae The authors report new findings on the distribution of Speonemadus maroccanus (Jeannel, 1936, Nargus (Demochrus rufipennis (Lucas, 1846, Choleva (Choleva kocheri Henrot, 1962 and Catops fuscus fuscoides Reitter, 1909. The geonemy of these species is updated and the research is illustrated with maps of their distribution.

  15. Release and distribution of Lilioceris cheni (Coleoptera: Chrysomelidae), a biological control agent of air potato (Dioscorea bulbilfera: Dioscoreaceae), in Florida

    Science.gov (United States)

    From 2012 to 2015, 429,668 Lilioceris cheni Gressit and Kimoto (Coleoptera: Chrysomelidae) were released in Florida for biological control of air potato [Dioscorea bulbilfera L. (Dioscoreaceae)]. The spatial distribution of releases was highly aggregated, with several areas of high density releases ...

  16. A NOVEL CADHERIN-LIKE GENE FROM WESTERN CORN ROOTWORM, DIABROTICA VIRGIFERA VIRGIFERA (COLEOPTERA: CHRYSOMELIDAE), LARVAL MIDGUT TISSUE

    Science.gov (United States)

    A cadherin-like gene and its mRNA were cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe. The full length cDNA (5371 bp in length) encodes an open reading frame for a 1688 amino ...

  17. Development and characterization of 11 microsatellite markers in the root-gall-forming weevil, Ceutorhynchus assimilis (Coleoptera: Curculionidae)

    Science.gov (United States)

    The host race of Ceutorhynchus assimilis (Coleoptera: Curculionidae) that specifically develops on Lepidium draba (Brassicales: Brassicaceae), an invasive weed in North America, is being considered for use as a biocontrol agent. Because there are other races that attack other plants, it is important...

  18. Sorghum halepense (L.) Persoon (Poaceae), a new larval host for the South American corn rootworm Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Diabrotica speciosa is a South American corn rootworm (Coleoptera: Chrysomelidae: Galerucinae) the adult of which is a pest on many crop and ornamental plants. The list of known larval hosts, however, is limited to maize, wheat, potatoes and peanuts. In March, 2005, larvae of D. speciosa were found ...

  19. Seasonal flight activity and distribution of metallic woodboring beetles (Coleoptera: Buprestidae) collected in North Carolina and Tennessee

    Science.gov (United States)

    Metallic wood boring insects (Coleoptera: Buprestidae) species are responsible for high levels of host plant injury to deciduous shade and flowering trees in commercial nurseries, urban forests, and managed landscapes. Ornamental plant producers in the southeastern U.S. have ranked borers, includin...

  20. Acoustic detection of Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae) and Nasutitermes luzonicus (Isoptera: Termitidae) in palm trees of urban Guam

    Science.gov (United States)

    Adult and larval Oryctes rhinoceros (L) (Coleoptera: Scarabaeidae: Dynastinae) were acoustically detected in live and dead palm trees and logs in recently invaded areas of Guam, along with Nasutitermes (Isoptera: Termitidae), and other small, sound-producing invertebrates and invertebrates. The sou...

  1. Records of unsuccessful attack by Anoplophora glabripennis (Coleoptera: Cerambycidae) on broadleaf trees of questionable suitability in Ontario

    Science.gov (United States)

    Discovery of the non-native Anoplophora glabripennis Motschulsky (Coleoptera: Cerambycidae) in Ontario, Canada, in 2003 led to the implementation of an eradication program. The plan consisted of removing all infested trees and all trees within 400 m of an infested tree belonging to a genus consider...

  2. First record of the agave snout weevil, Scyphophorus acupunctatusGyllenhal (Coleoptera: Curculionidae: Dryophthorinae), in Puerto Rico

    OpenAIRE

    Setliff, Gregory P.; Anderson, Jesse A.

    2011-01-01

    The agave snout weevil, Scyphophorus acupunctatus Gyllenhal (Coleoptera: Curculionidae: Dryophthorinae), is reported from Puerto Rico for the first time. It was collected on feral sisal, Agave sisalana Perrine (Agavaceae), in the Guánica Dry Forest Reserve in the southwestern part of the island.

  3. Redescrição e transferência do gênero Fregolia Gounelle, 1911 para Callidiopini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Jose Ricardo M. Mermudes

    2002-01-01

    Full Text Available Redescription and transference of the genus Fregolia Gounelle, 1911 to Callidiopini (Coleoptera, Cerambycidae. The genus Fregolia is transferred from Cleomenini Lacordaire, 1869 to Callidiopini Lacordaire, 1869. The genus and its type species, Fregolia listropteroides Gounelle, 1911, the only known species to the genus, are redescribed including characters of the mouth pieces, endosternites, wing venation, and male and female terminalia.

  4. Resistance in Cultivated Sunflower Germplasm to the Red Sunflower Seed Weevil (Coleoptera: Curculionidae) in the Northern Great Plains

    Science.gov (United States)

    A 6-year field study evaluated 52 sunflower, Helianthus annuus L., accessions, 20 breeding lines, and 9 interspecific crosses for resistance to infestation by naturally occurring populations of the red sunflower seed weevil, Smicronyx fulvus LeConte (Coleoptera: Curculionidae). Germplasm with potent...

  5. Het lieveheersbeestje Harmonia axyridis in Nederland: een aanwinst voor onze fauna of een ongewenste indringer (Coleoptera: Coccinellidae)?

    NARCIS (Netherlands)

    Cuppen, J.; Heijerman, Th.; Wielink, van P.; Loomans, A.

    2004-01-01

    Harmonia axyridis in the Netherlands: a gain for the fauna or an unwanted intruder (Coleoptera: Coccinellidae)? The coccinellid Harmonia axyridis (Pallas, 1773), a well-known aphid predator originating from Asia, was found for the first time in the Netherlands (Heilige Landstichting near Nijmegen) i

  6. Involvement of larder beetles (Coleoptera: Dermestidae) on human cadavers: a review of 81 forensic cases.

    Science.gov (United States)

    Charabidze, Damien; Colard, Thomas; Vincent, Benoit; Pasquerault, Thierry; Hedouin, Valery

    2014-11-01

    From 1994 to 2013, French forensic entomology laboratories investigated 1,093 cases. Larder beetles (Coleoptera: Dermestidae) were observed in 81 (7.5%) of these cases. To describe and analyze these 81 cases, eight parameters were used: city, location (indoor or outdoor), decay stage (fresh, decay, or dry), dermestid species and instar (adults and/or larvae), presence of living calliphorid larvae, presence of calliphorid pupae or adults, and presence of other necrophagous species. Eight Dermestidae species were observed: Dermestes frischii (42% of cases), Dermestes undulatus (35.8%), Dermestes peruvianus (12.3%), Dermestes lardarius (9.9%), Dermestes haemorrhoidalis (8.6%), Dermestes maculatus (7.4%), Dermestes bicolor (3.7%), and Dermestes ater (1.2%). Larder beetles primarily developed on human cadavers in outdoor locations in areas with a dry climate and were never reported in oceanic areas (which are characterized by frequent rainfall and high ambient humidity). The number of dermestid species on a single corpse never exceeded three. Typically, one species was found per corpse. Species differed between indoor and outdoor cases, with D. frischii and D. undulatus dominant in outdoor cases, while D. peruvianus dominant in indoor cases. Calliphoridae was found in 88% of the cases, while Hydrotaea and Piophilidae were observed 40% of the time. Regarding Coleoptera, Necrobia spp. (Coleoptera: Cleridae) was observed in 46% of the cases. Lastly, we observed a typical decomposition pattern, with preferential feeding areas on the face, hands, and feet (i.e., the extremities). Pupation chambers on or inside the bones were not observed.

  7. Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico

    Directory of Open Access Journals (Sweden)

    Sonia Maria Noemberg Lazzari

    2006-06-01

    Full Text Available Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico. O objetivo desta pesquisa foi avaliar o efeito do resfriamento artificial de grãos de arroz para o controle de coleópteros-praga. O ar frio foi insuflado pelo sistema de aeração em um silo metálico com arroz-em-casca. A avaliação do tratamento foi feita quinzenalmente usando armadilhas caladores. As espécies de Coleoptera capturadas foram: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16,5% e Sitophilus spp. (0,5%. Aos 28 dias, a temperatura média da massa de grãos era de 15ºC, e o número médio de insetos havia diminuído 76,8%. A aplicação de ar frio manteve as populações sob controle por aproximadamente 60 dias. Os resultados do monitoramento dos insetos e da temperatura indicaram que um novo ciclo de ar frio deveria ser aplicado nesse período para manter as populações sob controle. Também o manejo adequado da massa de grãos faz-se necessário para garantir resultados satisfatórios do resfriamento artificial.Artificial chilling to control Coleoptera in paddy rice stored in metallic silo. The objective of this research was to evaluate the effect of artificial chilling for the control of coleopterans in stored paddy rice. The cold air was insufflated through the aeration system of a metallic silo with paddy rice. Evaluation of insect number was made every 15 days using probe traps. The species of Coleoptera captured were: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16.5% and Sitophilus spp. (0.5%. By the 28th day the average temperature of the grain mass was 15ºC, and the mean number of insects decreased 76.8%. The cold air application kept the insect populations under control for approximately 60 days. The results of temperature and insect monitoring indicated that a new cycle of cold air should be applied by that time to keep the populations under

  8. Inconspicuous structural coloration in the elytra of beetles Chlorophila obscuripennis (Coleoptera)

    Science.gov (United States)

    Liu, Feng; Yin, Haiwei; Dong, Biqin; Qing, Youhua; Zhao, Li; Meyer, Serge; Liu, Xiaohan; Zi, Jian; Chen, Bin

    2008-01-01

    The elytra of male beetles Chlorophila obscuripennis (Coleoptera) display an inconspicuous iridescent bluish green color. By structural characterizations we find that the outermost elytral surface comprises a sculpted multilayer, which is the origin of structural coloration. In elytra both structural green and cyan colors are observed which arise from the modulations imposed on the multilayer, leading to a bluish green color by color mixing. The adoption of the sculpted multilayer can render structural coloration inconspicuous, which could be advantageous for camouflage. In addition, it can cause light emergence at nonspecular angles.

  9. Gross anatomy of central nervous system in firefly, Pteroptyx tener (Coleoptera: Lampyridae)

    Science.gov (United States)

    Hudawiyah, Nur; Wahida, O. Nurul; Norela, S.

    2015-09-01

    This paper describes for the first time the organization and fine structure of the central nervous system (CNS) in the fireflies, Pteroptyx tener (Coleoptera: Lampyridae). The morphology of the CNS was examined by using Carl Zeiss AxioScope A1 photomicroscope with iSolution Lite software. Some specific structural features such as the localization of protocerebrum, deutocerebrum and tritocerebrum in the brain region were analyzed. Other than that, the nerve cord and its peripheral structure were also analyzed. This study suggests that, there is a very obvious difference between male and female central nervous system which illustrates that they may differ in function in controlling physiological and behavioral activities.

  10. Australian marsh beetles (Coleoptera: Scirtidae). 2. Pachycyphon, a new genus of presumably terrestrial Australian Scirtidae.

    Science.gov (United States)

    Zwick, Peter

    2013-01-01

    The genus Pachycyphon is erected for marsh beetles (Coleoptera: Scirtidae) from tropical rainforests in Queensland, Australia. The following species are included: P. corpulentus sp. n., P. crassus sp. n., P. elegans sp. n., P. funicularis sp. n., P. gravis sp. n., P. monteithi sp. n., P. obesus sp. n., P. pinguis sp. n., P. serratus sp. n., P. turgidus sp. n. (the type species). Females are wingless and have fossorial ovipositors, a terrestrial larval life is therefore hypothesized. Pachycyphon is compared with other genera, especially probable relatives from temperate rainforests in southeastern Australia.

  11. Invertebrate fauna (Coleoptera, Collembola, Diplopoda, Isopoda collected in the karst areas of the Aninei - Locvei Mountains

    Directory of Open Access Journals (Sweden)

    Andrei Giurginca

    2011-08-01

    Full Text Available The authors identified 132 species of invertebrates (14 Oniscidea, 25 Diplopoda,31 Collembola and 62 Coleoptera recently sampled (2001–2006 from the soil and subterranean (MSS and caves environments from the Banat Mountains. Some new,rare and endemic species are discussed. The seasonal changes of the species diversity in the superficial subterranean environments at 0.5 to 1 m in depth are for the first time presented for the Reşiţa – Moldova Nouă synclinorium. The characteristic and preferential species for the mesovoid shallow substratum (MSS, belonging to the analyzed taxa, are identified.

  12. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-09-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  13. Development of an attractant-baited trap for Oxythyrea funesta Poda (Coleoptera: Scarabaeidae, Cetoniinae).

    Science.gov (United States)

    Vuts, József; Imrei, Zoltán; Töth, Miklós

    2008-01-01

    In electroantennographic tests isosafrol, methyl salicylate, (+/-)-lavandulol, geraniol, (E)-anethol, and beta-ionone evoked the largest responses from antennae of female or male Oxythyrea funesta (Coleoptera: Scarabaeidae, Cetoniinae) adult beetles. In field trapping tests in Hungary the 1:1 blend of (+/-)-lavandulol and 2-phenylethanol attracted significantly more adult O. funesta than the single compounds. The addition of (E)-anethol, a previously described attractant for the species, was without effect. There was no difference in the responses of male or female beetles. The binary 2-phenylethanol/(+/-)-lavandulol bait described, in this study is recommended for the use in traps of O. funesta for agricultural purposes.

  14. A review of the natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries.

    Science.gov (United States)

    Di Iorio, Osvaldo

    2014-01-01

    A compilation of the known natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries is provided. Food items of adult Cetoniinae include pollen and/or nectar (flower visitors), sap and/or slime flux, ripened fruits on plants, green tissues and leaves, and honey. Of the 36 species of Cetoniinae from Argentina, food items are known only for 11 species (30.5%). Attraction to light and bait-traps, adult activity periods, vertebrate predators, and the occurrence in bird nests are presented and discussed. Other insects that share the same food sources and bait-traps with Cetoniinae are mentioned. PMID:24869870

  15. Espécies de Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini ocorrentes na Bolívia

    Directory of Open Access Journals (Sweden)

    Maria Helena M. Galileo

    2013-03-01

    Full Text Available Species of Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini occurring in Bolivia. The genus Gorybia (Cerambycinae, Piezocerini consists of 45 described species with seven species recorded from Bolivia. Nine new species are described herein from Bolivia: G. abnormalis sp. nov.; G. alveolata sp. nov.; G. asyka sp. nov.; G. florida sp. nov.; G. inarmata sp. nov.; G. longithorax sp. nov.; G. guenda sp. nov.; G. tuberosa sp. nov. and G. wappesi sp. nov. A key to the species now known to occur in Bolivia is included.

  16. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-06-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  17. PITYOPHAGUS QUERCUS REITTER, 1877, A NEW SAPROXYLIC SAP BEETLE FOR THE ITALIAN FAUNA (Coleoptera, Nitidulidae

    Directory of Open Access Journals (Sweden)

    Paolo Audisio

    2011-10-01

    Full Text Available During ecological investigations on saproxylic beetle communities of central Italy (Latium, at Bosco Polverino (a mixed evergreen/deciduous forest fragment dominated by cork oaks, and at Allumiere (a small fragment of beech forest surrounded by turkey oak stands, the authors found three specimens of Pityophagus quercus Reitter, 1877 (Coleoptera, Nitidulidae. These are the first known records of this species in Italy, and the first one in association with an evergreen oak, Quercus suber. This discovery led us to review both bionomical and faunistic data so far available on this exceedingly rare and poorly known species.

  18. Redescription of Platynaspis flavoguttata (Gorham (Coleoptera, Coccinellidae and notes on nomenclature of Platynaspis kapuri Chakraborty & Biswas

    Directory of Open Access Journals (Sweden)

    J. Poorani

    2014-05-01

    Full Text Available Platynaspis flavoguttata (Gorham (Coleoptera: Coccinellidae is redescribed and the male genitalia are illustrated for the first time. It is also recorded from Sri Lanka for the first time. Platynaspis bimaculata (Hoang, 1983 is a new junior synonym of Platynaspis bimaculata Pang & Mao, 1979 (new synonym. Platynaspis kapuri Chakraborty & Biswas, 2000, the replacement name for Platynaspis bimaculata Pang & Mao, 1979 established by Ukrainsky (2007, is also the new replacement name for Platynaspis bimaculata (Hoang, 1983, as both are junior homonyms of Platynaspis bimaculata Weise, 1888 besides being synonyms. Platynaspis hoangi Ukrainsky (2007 is an unnecessary replacement name for P. bimaculata (Hoang.

  19. CONTRIBUTIONS TO THE KNOWLEDGE OF THE SPECIES OF BEETLES (INSECTA: COLEOPTERA FROM WHEAT CROP

    Directory of Open Access Journals (Sweden)

    Mihai Tălmaciu

    2016-07-01

    Full Text Available This paper presents the species of beetles (Insecta: Coleoptera between in wheat crops from Tişiţa, in Vrancea county. The observations were made in a crop of wheat in 2013, who were placed the soil traps type Barber, during the two months, May and June. It was used three variants: • Variant 1 - consumption wheat untreated • Variant 2 - consumption wheat treaty • Variant 3 - treated wheat seed The gathering of samples from the traps was done periodically, every 12-15 days. The most species frequent gathered was: Pentodon idiota, Epicometis hirta, Opatrum sabulosum, Phyllotreta atra, Phyllotreta nemorum, Tanymecus dilaticollis.

  20. Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e Diptera Bacillus thuringiensis strains effective against insects of Lepidoptera, Coleoptera and Diptera orders

    Directory of Open Access Journals (Sweden)

    Lílian Botelho Praça

    2004-01-01

    Full Text Available O objetivo deste trabalho foi selecionar entre 300 estirpes de Bacillus thuringiensis as efetivas simultaneamente contra larvas de Spodoptera frugiperda J.E. Smith e Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae, Anthonomus grandis Boheman (Coleoptera: Curculionidae, Aedes aegypti Linnaeus e Culex quinquefasciatus Say (Diptera: Culicidae. Foram selecionadas duas estirpes de B. thuringiensis, denominadas S234 e S997, que apresentaram atividade contra as três ordens de insetos. As estirpes foram caracterizadas por métodos morfológicos, bioquímicos e moleculares. As mesmas apresentaram duas proteínas principais de 130 e 65 kDa, produtos de reação em cadeia da polimerase de tamanho esperado para a detecção dos genes cry1Aa, cry1Ab, cry1Ac, cry1B e cry2 e cristais bipiramidais, cubóides e esféricos.The aim of this work was to select among 300 strains of Bacillus thuringiensis those which are simultaneously effective against larvae of Spodoptera frugiperda J.E. Smith and Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae, Anthonomus grandis Boheman (Coleoptera: Curculionidae, Aedes aegypti Linnaeus and Culex quinquefasciatus Say (Diptera: Culicidae. Two strains of B. thuringiensis were selected, S234 and S997, which presented activity against those three insect orders. Both strains were characterized by morphological, biochemical and molecular methods. They have presented two main proteins with 130 and 65 kDa, polimerase chain reaction products with expected sizes for detection of the genes cry1Aa, cry1Ab, cry1Ac, cry1B and cry2 and bipiramidal, cubical and spherical crystals.

  1. Phylogenetically informative rearrangements in mitochondrial genomes of Coleoptera, and monophyly of aquatic elateriform beetles (Dryopoidea).

    Science.gov (United States)

    Timmermans, Martijn J T N; Vogler, Alfried P

    2012-05-01

    Mitochondrial gene order in Coleoptera has been thought to be conservative but a survey of 60 complete or nearly complete genomes revealed a total of seven different gene rearrangements (deletions, gene order reversals), mainly affecting tRNA genes. All of these were found to be limited to a single taxon or a subclade of Coleoptera. The phylogenetic distribution of a translocation of tRNA(Pro) in three species of elateriform beetles was investigated further by sequencing three nearly complete mitochondrial genomes (Dascillidae, Byrrhidae, Limnichidae) and ten additional individuals for a ∼1370 bp diagnostic fragment spanning the relevant region. Phylogenetic analysis consistently recovered the monophyly of families previously grouped in the contentious superfamily Dryopoidea, a group of approximately 10 beetle families with mainly aquatic lifestyles. The Byrrhidae (moss beetles) were not part of this lineage, although they may be its sister group, to recover the widely accepted Byrrhoidea. The tRNA(Pro) translocation was present in all members of Dryopoidea, but not in any other Elateriformia, providing independent support for this lineage and for a single origin of aquatic habits.

  2. The hydraulic mechanism in the hind wing veins of Cybister japonicus Sharp (order: Coleoptera).

    Science.gov (United States)

    Sun, Jiyu; Wu, Wei; Ling, Mingze; Bhushan, Bharat; Tong, Jin

    2016-01-01

    The diving beetles (Dytiscidae, Coleoptera) are families of water beetles. When they see light, they fly to the light source directly from the water. Their hind wings are thin and fragile under the protection of their elytra (forewings). When the beetle is at rest the hind wings are folded over the abdomen of the beetle and when in flight they unfold to provide the necessary aerodynamic forces. In this paper, the unfolding process of the hind wing of Cybister japonicus Sharp (order: Coleoptera) was investigated. The motion characteristics of the blood in the veins of the structure system show that the veins have microfluidic control over the hydraulic mechanism of the unfolding process. A model is established, and the hind wing extending process is simulated. The blood flow and pressure changes are discussed. The driving mechanism for hydraulic control of the folding and unfolding actions of beetle hind wings is put forward. This can assist the design of new deployable micro air vehicles and bioinspired deployable systems. PMID:27547607

  3. Ophiostoma species (Ascomycetes: Ophiostomatales) associated with bark beetles (Coleoptera: Scolytinae) colonizing Pinus radiata in northern Spain.

    Science.gov (United States)

    Romón, Pedro; Zhou, XuDong; Iturrondobeitia, Juan Carlos; Wingfield, Michael J; Goldarazena, Arturo

    2007-06-01

    Bark beetles (Coleoptera: Scolytinae) are known to be associated with fungi, especially species of Ophiostoma sensu lato and Ceratocystis. However, very little is known about these fungi in Spain. In this study, we examined the fungi associated with 13 bark beetle species and one weevil (Coleoptera: Entiminae) infesting Pinus radiata in the Basque Country of northern Spain. This study included an examination of 1323 bark beetles or their galleries in P. radiata. Isolations yielded a total of 920 cultures, which included 16 species of Ophiostoma sensu lato or their asexual states. These 16 species included 69 associations between fungi and bark beetles and weevils that have not previously been recorded. The most commonly encountered fungal associates of the bark beetles were Ophiostoma ips, Leptographium guttulatum, Ophiostoma stenoceras, and Ophiostoma piceae. In most cases, the niche of colonization had a significant effect on the abundance and composition of colonizing fungi. This confirms that resource overlap between species is reduced by partial spatial segregation. Interaction between niche and time seldom had a significant effect, which suggests that spatial colonization patterns are rarely flexible throughout timber degradation. The differences in common associates among the bark beetle species could be linked to the different niches that these beetles occupy. PMID:17668036

  4. Methodology for evaluating the insect growth regulator (IGR) methoprene incorporated into packaging films

    Science.gov (United States)

    The insect growth regulator methoprene has been impregnated onto various packaging materials to control stored product insects, and is labeled for use in this manner in the United States. Different methodologies were utilized to evaluate efficacy towards Tribolium castaneum (Herbst), the red flour b...

  5. Variation in susceptibility of laboratory and field strains of three stored-grain insect species to beta-cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to concrete surfaces

    Science.gov (United States)

    The efficacy of beta-cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to clean, concrete floors of empty bins prior to grain storage against field strains of stored-grain insects is unknown. We exposed adults of 16 strains of the red flour beetle, Tribolium castaneum (Herbst); 8 strains ...

  6. ESCARABAJOS TIGRE (COLEOPTERA: CICINDELIDAE DEL MUSEO ENTOMOLÓGICO FRANCISCO LUÍS GALLEGO: NUEVOS REGISTROS PARA DEPARTAMENTOS DE COLOMBIA TIGER BEETLES (COLEOPTERA: CICINDELIDAE AT THE ENTOMOLOGICAL MUSEUM FRANCISCO LUÍS GALLEGO: NEW RECORDS

    Directory of Open Access Journals (Sweden)

    Manuel Alejandro Ramírez Mora

    2008-06-01

    Full Text Available Se registran por primera vez las especies de escarabajos tigre (Coleoptera: Cicindelidae presentes en el Museo Entomológico Francisco Luis Gallego (MEFLG. Se identificaron 167 especimenes distribuidos en ocho géneros y 27 especies, se reportan 24 nuevos registros para diferentes departamentos de Colombia. Se señalan aspectos importantes de la taxonomía y sistemática del grupo. Además, se presentan comentarios biológicos y de distribución de las especies.Tiger beetles (Coleoptera: Cicindelidae species at the Museo Entomológico Francisco Luis Gallego (MEFLG are registered by first time. 167 specimens in 8 genus and 27 species were identified, 24 new records for different Colombian states are reported. Some important aspects of the group’s taxonomy and systematic are pointed. Additionally, species’ biological and distributional comments are presented.

  7. Especies de Megacerus (Coleoptera: Bruchidae y sus plantas hospederas en Yucatán Megacerus species (Coleoptera: Bruchidae and their host plants in Yucatán

    Directory of Open Access Journals (Sweden)

    Enrique Reyes

    2009-12-01

    Full Text Available Se registra la incidencia de Megacerus (Coleoptera: Bruchidae en semillas de 7 especies de Convolvulaceae de 6 sitios al norte de Yucatán. Las semillas de Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia y M. cissoides recolectadas en etapa de dispersión fueron colocadas en sobres limpios de papel y puestas en condiciones de laboratorio hasta la emergencia de los brúquidos. Se obtuvieron 1111 coleópteros pertenecientes a 5 especies: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, y M. (Serratibruchus cubiciformis. Las semillas de I. crinicalyx, M. aegyptia y M. cissoides fueron infestadas principalmente por M. (M. porosus, las de I. hederifolia e I. triloba por M. (M. cubiculus y las de I. nil por M. (M. tricolor. Todos los individuos de Megacerus (M. sp., fueron encontrados en semillas de J. penthanta. En el nivel génerico de hospedero se encontró que las semillas de Ipomoea fueron infestadas principalmente por M. (M. cubiculus y las de Merremia por M. (M. porosus. Estos resultados amplían el rango de plantas hospederas registrado para las especies de Megacerus y resaltan la estrecha asociación que existe entre un linaje de depredadores de semillas Bruchidae y plantas de la familia Convolvulaceae.Seed-attacking by Megacerus (Coleoptera: Bruchidae is reported for 7 Convolvulaceae species from 6 sites at the North of Yucatan. Seeds of Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia and M. cissoides were collected and placed in clean envelopes under laboratory conditions to collect all bruchids emerged from seeds. A total of 1111 coleopterans were hatched from seeds, and 5 taxa were identify: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, and M. (Serratibruchus cubiciformis. Seeds of I. crinicalyx, M. aegyptia and M. cissoides were attacked mainly by M. (M. porosus; I

  8. Longhorned Beetles Collection of the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey (Coleoptera,Cerambycidae)

    OpenAIRE

    ÖZDİKMEN, Hüseyin; ŞAHİN, Özlem

    2010-01-01

    In the present paper, specimens of Cerambycidae were examined in the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey. As a result of identification of these specimens, thirty-eight species and two subspecies belonging to twenty-eight genera of five subfamilies were determined. With this paper, new faunistic data and some zoogeographical evaluations were presented on longhorned beetles fauna (Coleoptera, Cerambycidae) of Turkey. The faunistic data in the pr...

  9. A new genus and species of Schizogyniidae (Acari: Mesostigmata) associated with carabid beetles (Coleoptera: Carabidae) from Ukraine.

    Science.gov (United States)

    Trach, Viacheslav A; Seeman, Owen D

    2014-04-29

    A new genus and species of Schizogyniidae (Acari: Mesostigmata: Celaenopsoidea), Euroschizogynium calvum gen. nov. and sp. nov., associated with Scarites terricola Bonelli, 1813 (Coleoptera: Carabidae) is described from Ukraine, representing the first record of the family from the Palaearctic. Fusura civica Valle & Fox, 1966 is moved out of the Schizogyniidae and placed into the Megacelaenopsidae. A new diagnosis for the family Schizogyniidae and a key to genera are provided.

  10. Uji Efektifitas Beberapa Insektisida Nabati Terhadap Mortalitas Hama Sitophilus zeamais Motsch. (Coleoptera; Curculionidae) Pada Benih Jagung (Zea mays)

    OpenAIRE

    Subhan, Fadillah

    2011-01-01

    Fadillah Subhan, "Test Effectiveness of Some Insecticides Against Plant Pests Sitophilus zeamais Motsch Mortality. (Coleoptera: Curculionidae) In the Seed of Corn (Zea mays). "It was under supervised by Yuswani Pangestiningsih and Fatimah Zahara. S. zeamais is a pest of corn in storage that causes substantial yield losses, one of which effective control is the use of botanical insecticides. Testing the effectiveness of some botanical insecticides on mortality of S. zeamais on maize seed plant...

  11. A review of Elocomosta Hansen with a description of a new species with reduced eyes from China (Coleoptera, Hydrophilidae, Sphaeridiinae)

    Science.gov (United States)

    Lin, Renchao; Jia, Fenglong; Fikáček, Martin

    2016-01-01

    Abstract A new species of the genus Elocomosta Hansen, 1989 (Coleoptera: Hydrophilidae: Sphaeridiinae: Coelostomatini), Elocomosta lilizheni sp. n., is described from Guangxi Province, China. It is compared in detail with the only other known species of the genus, Elocomosta nigra Hansen, 1989 from Borneo, and the genus is diagnosed from the remaining coelostomatine genera. The new species is unusual among Hydrophilidae by having extremely reduced eyes. PMID:27551232

  12. Synergistic effects of chlorpyrifos with piperonyl butoxide (pbo) against the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

    OpenAIRE

    Akter Mst Yeasmin; Talukdar Muhammad Waliullah; ASM Shafiqur Rahman

    2014-01-01

    Objective: To investigate the co-toxicity and co-efficient activity of Chlorpyrifos (Dursban 20EC), an organophosphate and Piperonyl butoxide (PBO) against the lesser meal worm Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) (A. diaperinus). Methods: The repellent activity was carried out by the residual film assay technique. Statistically the dose mortality relationship was expressed as a median lethal dose (LD50) by the probit analysis. The regression lines and isoboles were ...

  13. The lectotype of Goliathus drurii Westwood, 1837 (Coleoptera: Scarabaeidae: Cetoniinae) in the Macleay Museum, University of Sydney, Australia.

    Science.gov (United States)

    Blackburn, Robert

    2016-01-01

    The lectotype of Goliathus drurii Westwood (Coleoptera: Scarabaeidae: Cetoniinae) has been located to the Macleay Museum, University of Sydney, Australia. Collected in 1775, this specimen represents the second species of Goliathus to be illustrated in European literature, however eluding a proper species description until 1837. This important specimen, overlooked by previous curators, represents another portion of the Dru Drury collections purchased by Alexander McLeay now held in the Macleay Museum. PMID:27615919

  14. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    OpenAIRE

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineens...

  15. Natural history of Belonuchus Nordmann spp. and allies (Coleoptera: Staphylinidae) in Heliconia L. (Zingiberales: Heliconiaceae) flower bracts

    OpenAIRE

    Frank, J. Howard; Barrera, Roberto

    2010-01-01

    Adults, and in some species larvae, of several members of Belonuchus Nordmann (Coleoptera: Staphylinidae: Staphylininae) and a few related genera seem to be to various degrees consistently associated with flower bracts of the genus Heliconia (Zingiberales: Heliconiaceae). They are predators and eat various dipterous and lepidopterous larvae in that habitat. Adults of at least Belonuchus cephalotes (Sharp) and Odontolinus fasciatus Sharp are able to immerse completely in water to capture larva...

  16. The mortality of Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae induced by powdered plants

    Directory of Open Access Journals (Sweden)

    Kłyś Małgorzata

    2015-01-01

    Full Text Available The aim of this study was to investigate whether powdered plants of different species namely: peppermint Mentha piperita (L. (Lamiaceae, wormwood Artemisia absinthium (L. (Asteraceae, common sage Salvia officinalis (L. (Lamiaceae, allspice Pimenta dioica (Linnaeus et Merrill (Myrtaceae and common garlic Allium sativum (L. (Amaryllidaceae, added to semolina using concentrations of 1.23, 3.61, and 5.88%, influence the mortality rate in the saw-toothed grain beetle Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae. Experiments were conducted in a laboratory at 28°C and relative humidity 60±5%. At the concentration of 1.23%, allspice seeds caused the highest mortality amongst the saw-toothed grain beetle. When concentrations of 3.61 and 5.88% were used, sage, peppermint and wormwood caused the highest statistically significant mortality of O. surinamensis

  17. Carpophilus zeaphilus, a new sap beetle species acclimatized in Italy (Coleoptera: Nitidulidae

    Directory of Open Access Journals (Sweden)

    Paolo Audisio

    2015-06-01

    Full Text Available Carpophilus zeaphilus Dobson, 1969 (Coleoptera, Nitidulidae, Carpophilinae is an Afrotropical species that has become widespread in Sub-Saharan Africa, the Arabian Peninsula, and southern Mediterranean areas in recent years. The species was first recorded from Europe in Portugal and Spain nearly thirty years ago, and it was later intercepted in Sicily near Trapani in 1991. A few specimens of this species were collected in April, 2015 in a sparsely forested area near Rome, which suggests a recent acclimatization into peninsular Italy. Specimens were taken on flowering trees of Prunus spinosa L. (Rosaceae, an unusual occurrence for most introduced species of Carpophilinae that are normally associated with rotten fruit and other decomposing vegetal matter.

  18. Beetles (Insecta, Coleoptera) associated with pig carcasses exposed in a Caatinga area, Northeastern Brazil.

    Science.gov (United States)

    Santos, W E; Alves, A C F; Creão-Duarte, A J

    2014-08-01

    The species richness, abundance and seasonality of Coleoptera fauna associated with pig carcasses exposed in a Caatinga area were examined. Tray, pitfall and modified Shannon traps were settled together to collect these insects during two seasons (dry and rainy). 4,851 beetles were collected, belonging to 19 families and 88 species. Staphylinidae (2,184) and Histeridae (1,264) were the most abundant families and accounted for 71.1% of the specimens collected. Scarabaeidae (15) showed the highest species richness. The most abundant species were Atheta iheringi Bernhauer, 1908 (Staphylinidae) (1,685), Euspilotus sp. (Histeridae) (461), Stelidota geminata (Say, 1825) (Nitidulidae) (394), Xerosaprinus diptychus (Marseul, 1855) (Histeridae) (331) and Dermestes maculatus De Geer, 1774 (Dermestidae). Amongst these species, X. diptychus showed to be strongly influenced by seasonality, since 96.1% of the specimens were collected during the dry season.

  19. The tiger beetles (Coleoptera: Carabidae, Cicindelinae) of Angola: a descriptive catalogue and designation of neotypes.

    Science.gov (United States)

    Serrano, Artur R M; Capela, Rúben A

    2013-11-01

    An annotated catalogue of the species and subspecies of tiger beetles (Coleoptera: Carabidae, Cicindelinae) hitherto known from Angola is given. A total of 89 forms (74 species and 15 subspecies) is recorded from this southwestern country of Africa. Within this assemblage there are 31 endemic forms (33.3%). Some species are represented by only the holotype specimen (some without locality) or the type series. Others were recorded based on a single specimen. Records for six species previously unknow from Angola are given: Foveodromica sp. n. 1, Foveodromica sp. n. 2, Ophryodera rufomarginata bradshawi Péringuey, 1888, Elliptica muata parallelestriata (W. Horn, 1923), Lophyra differens (W. Horn, 1892) and Myriochila jucunda (Péringuey, 1892). A historical review, as well as some considerations on the distribution and conservation status of these beetles in Angola are also presented.

  20. Effects of pitfall trap preservative on collections of carabid beetles (Coleoptera: Carabidae)

    Science.gov (United States)

    McCravy, K.W.; Willand, J.E.

    2007-01-01

    Effects of six pitfall trap preservatives (5% acetic acid solution, distilled water, 70% ethanol, 50% ethylene glycol solution, 50% propylene glycol solution, and 10% saline solution) on collections of carabid beetles (Coleoptera: Carabidae) were studied in a west-central Illinois deciduous forest from May to October 2005. A total of 819 carabids, representing 33 species and 19 genera, were collected. Saline produced significantly fewer captures than did acetic acid, ethanol, ethylene glycol, and propylene glycol, while distilled water produced significantly fewer captures than did acetic acid. Significant associations between numbers of captures and treatment were seen in four species: Amphasia interstitialis (Say), Calathus opaculus LeConte, Chlaenius nemoralis Say, and Cyclotrachelus sodalis (LeConte). Results of this study suggest that type of preservative used can have substantial effects on abundance and species composition of carabids collected in pitfall traps.

  1. Discovery of mycangia and the associated xylose-fermenting yeasts in stag beetles (Coleoptera: Lucanidae)

    Science.gov (United States)

    Tanahashi, Masahiko; Kubota, Kôhei; Matsushita, Norihisa; Togashi, Katsumi

    2010-03-01

    Most wood-feeding insects need an association with microbes to utilize wood as food, and some have special organs to store and convey the microbes. We report here the discovery of the microbe-storage organ (mycangium) in stag beetles (Coleoptera: Lucanidae), which develop in decayed wood. The mycangium, which was discovered in the abdomen, is present in all adult females of 22 lucanid species examined in this study, but absent in adult males. By contrast, adult insects of both sexes of selected Passalidae, Geotrupidae, and Scarabaeidae, which are related to Lucanidae, lacked mycangia similar to those of the lucanid species. Yeast-like microbes were isolated from the mycangium of five lucanid species. DNA sequence analyses indicate that the microbes are closely related to the xylose-fermenting yeasts Pichia stipitis, Pichia segobiensis, or Pichia sp. known from the gut of a passalid species.

  2. Synthesis, antifeedant activity against Coleoptera and 3D QSAR study of alpha-asarone derivatives.

    Science.gov (United States)

    Łozowicka, B; Kaczyński, P; Magdziarz, T; Dubis, A T

    2014-01-01

    For the first time, a set of 56 compounds representing structural derivatives of naturally occurring alpha-asarone as an antifeedants against stored product pests Sitophilus granarius L., Trogoderma granarium Ev., and Tribolium confusum Duv., were subjected to the 3D QSAR studies. Three-dimensional quantitative structure-activity relationships (3D-QSAR) for 56 compounds, including 15 newly synthesized, were performed using comparative molecular field analysis s-CoMFA and SOM-CoMSA techniques. QSAR was conducted based on a combination of biological activity (against Coleoptera larvae and beetles) and various geometrical, topological, quantum-mechanical, electronic, and chromatographic descriptors. The CoMSA formalism coupled with IVE (CoMSA-IVE) allowed us to obtain highly predictive models for Trogoderma granarium Ev. larvae. We have found that this novel method indicates a clear molecular basis for activity and lipophilicity. This investigation will facilitate optimization of the design of new potential antifeedants. PMID:24601760

  3. Ground beetle (Coleoptera: Carabidae) assemblages in narrow hedgerows in a Danish agricultural landscape

    DEFF Research Database (Denmark)

    Lövei, G. L.; Magura, T.

    2015-01-01

    beetle assemblages. The number of ground beetle individuals and species were significantly the highest in the hawthorn hedges and significantly decreased from the hedges with rowan toward the spruce hedges. The elevated number of ground beetle individuals and species in the hawthorn hedges were due......The role of hedgerows in supporting ground beetles (Coleoptera: Carabidae) in a Danish agricultural landscape was examined. Nine old, well established single-row hedges were selected for the study, three each of a native species (hawthorn, Crataegus monogyna), a non-native deciduous one (rowan...... to the forest specialist species, as the number of forest specialist ground beetle individuals and species were significantly higher in the hawthorn hedges compared to the hedges with rowan and spruce. Differences in the number of the grassland and the cropland specialist ground beetle individuals and species...

  4. The scarab beetle tribe Pentodontini (Coleoptera: Scarabaeidae: Dynastinae) of Colombia: taxonomy, natural history, and distribution.

    Science.gov (United States)

    López-García, Margarita M; Gasca-Álvarez, Héctor J; Amat-García, Germán

    2015-11-27

    Pentodontini is the most diverse tribe of Dynastinae (Coleoptera: Scarabaeidae), and most of the genera are restricted to a single biogeographic region. In this work, the taxonomic composition of the Pentodontini in Colombia was determined, and genera and species were diagnosed based on external morphology and male genitalia. Records of 1,580 specimens from 31 departments and 398 localities in Colombia were obtained from 24 species in the genera Bothynus Hope, Denhezia Dechambre, Euetheola Bates, Hylobothynus Ohaus, Oxyligyrus Arrow, Parapucaya Prell, Pucaya Ohaus, and Tomarus Erichson. Oxyligyrus cayennensis Endrödi, Tomarus cicatricosus (Prell), and T. pullus (Prell) are reported for the first time from Colombia. Pucaya punctata Endrödi is reduced to synonymy with Pucaya pulchra Arrow. Possible changes in the classification of Denhezia Dechambre are discussed. Dichotomous keys are provided for Colombian genera and species. Taxonomic descriptions and distribution maps are included for all species.

  5. Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes

    Energy Technology Data Exchange (ETDEWEB)

    Kukol, J.J.; Martin, M.M.

    1986-05-01

    Larvae of the balsam fir sawyer, Monochamus marmorator Kby. (Coleoptera, Cerambycidae), contain midgut digestive enzymes active against hemicellulose and cellulose. Cellulases from larvae fed on balsam fir wood infected with the fungus, Trichoderma harzianum Rifai (Deuteromycetes, Moniliales, Moniliaceae), were found to be identical to those of the cellulase complex produced by this fungus when compared using chromatography, electrophoresis, and isofocusing. When larvae are maintained on a fungusfree diet, their midgut fluids lack cellulolytic activity, and they are unable to digest cellulose. Cellulolytic capacity can be restored by feeding the larvae wood permeated by fungi. We conclude that the enzymes which enable M. marmorator larvae to digest cellulose are not produced by the larvae. Instead, the larvae acquire the capacity to digest cellulose by ingesting active fungal cellulases while feeding in fungus-infected wood.

  6. A importância forense de Oxelytrum discicolle (Brullé, 1840) (Coleoptera, Silphidae)

    OpenAIRE

    Camila Kraiczyi Kotzko; V. Costa-Silva; E. G. Silva

    2015-01-01

    Oxelytrum discicolle Brullé, 1840 é a espécie mais coletada de Silphidae (Coleoptera) na América Latina, constituindo um indicador forense na região Neotropical. O objetivo do trabalho é reunir informações sobre biologia e distribuição de O. discicolle, além de ferramentas para utilização desse besouro como indicador forense em casos de morte de humanos. O. discicolle é um besouro de grande porte, de cor preta com um colar vermelho. Larvas da espécie são necrófagas, já os adultos são onívoros...

  7. NEW CONTRIBUTION CONCERNING THE MASS REARING OF TANYMECUS DILATICOLLIS GYLL (COLEOPTERA: CURCULIONIDAE UNDER CONTROLLED CONDITIONS

    Directory of Open Access Journals (Sweden)

    Traian MANOLE

    2013-12-01

    Full Text Available Controlled growth in the laboratory of some species of insect’s are justified both scientifically and practically. Due to the economic importance that the corn crop pest Tanymecus dilaticollis Gyll. (Coleoptera: Curculionidae had in Romania like the main pest of maize crops we tried to obtain successive generations under environment controlled of the insect mentioned. In this paper we aim to improve the efficiency of the multiplication of the species with respect to two aspects: attending larval and multiplication high efficiency. The coefficient value of 20, 90 obtained by us indicate a considerable increase in performance compared to previous attempts multiplication thus increasing your chances of recovery of this method of mass multiplication.

  8. What do we know about winter active ground beetles (Coleoptera, Carabidae in Central and Northern Europe?

    Directory of Open Access Journals (Sweden)

    Radomir Jaskula

    2011-05-01

    Full Text Available This paper summarizes the current knowledge on winter active Carabidae in Central and Northern Europe. In total 73 winter active species are listed, based on literature and own observations. Ground beetles are among the three most numerous Coleoptera families active during the autumn to spring period. The winter community of Carabidae is composed both of larvae (mainly autumn breeding species and adults, as well as of epigeic species and those inhabiting tree trunks. Supranivean fauna is characterized by lower species diversity than the subnivean fauna. The activity of ground beetles decreases in late autumn, is lowest during mid-winter and increases in early spring. Carabidae are noted as an important food source in the diet of insectivorous mammals. They are also predators, hunting small winter active invertebrates.

  9. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae in Cotton

    Directory of Open Access Journals (Sweden)

    P. G. Tillman

    2012-01-01

    Full Text Available Lady beetles (Coleoptera: Coccinellidae prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L., Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas, Coleomegilla maculata (De Geer, Cycloneda munda (Say, and Olla v-nigrum (Mulsant were found in sorghum over both years. Lady beetle compositions in sorghum and cotton and in yellow pyramidal traps were similar. For both years, density of lady beetles generally was higher on cotton with sorghum than on control cotton. Our results indicate that sorghum was a source of lady beetles in cotton, and thus incorporation of a sorghum habitat in farmscapes with cotton has great potential to enhance biocontrol of insect pests in cotton.

  10. Herbst矫治器在不同重建时对口颌肌肉 和韧带约束反力的影响%The Influences Upon the Passive Tensile of the Masticatory Muscles and Ligaments by Herbst Appliance Under Various Bite ReconstructionA Three Dimensional Finite Element Analysis

    Institute of Scientific and Technical Information of China (English)

    宋锦; 赵志河; 陈君楷; 胡林华; 蒋文涛; 樊瑜波

    2001-01-01

    Objective:This study aimed to provide some biomechanical references for the clinical use and improvement of Herbst appliance. Methods: The three-dimensional model of the ′Temporomandibular joint mandible Herbst appliance system′was set up by SUPER SAP software (version 9.3). On this model, the passive tensile in the masticatory muscles and ligaments were analyzed under various bit reconstruction designed according to specified advanced displacement and vertical bite opening. Results: When Herbst appliance drove the mandible forward, there was not any tensile in the medial, lateral pterygoid and the collateral ligament, while the temporalis, the deep paret of masseter, the stylomandibular ligament, and the sphenomandibular ligament were passively drawn. Under various bite reconstruction, the passive tensile in the medial temporalis, the posterior temporalis, the stylomandibular ligament and the sphenomandibular ligament increased with the amount of the advancement of the mandible; The passive tensilein the posterior temporalis and the deep part of masseter increased with the amount of vertical bite opening of the mandible. Conclusion: The StL, SpL, PT and AT played an important role in functional reconstruction of mandible by Herbst. All five group of bite reconstruction (3~7mm advancement, 4~2mm vertical bite opening of the mandible) designed by this study can be selected in clinic according to the patient′s capa- bility of adaptation, the extent of malocclusion and the potential and direction of growth.%目的:研究不同重建情况下相关口颌肌肉和韧带的约束反力变化规律,为Herbst矫治器临床应用提供相关依据。方法:在已建立的下颌骨硬组织三维有限元模型基础上附加Herbst矫治器,建立颞下颌关节—下颌骨—Herbst矫治器系统的三维正交各向同性有限元模型,根据临床和相关文献设计重建的类型,通过计算机计算相关口颌肌肉和韧带的约束反力。结

  11. Odor-baited trap trees: a new approach to monitoring plum curculio (Coleoptera: Curculionidae).

    Science.gov (United States)

    Prokopy, Ronald J; Chandler, Bradley W; Dynok, Sara A; Piñero, Jaime C

    2003-06-01

    We compared a trap approach with a trap-tree approach to determine the need and timing of insecticide applications against overwintered adult plum curculios, Conotrachelus nenuphar (Herbst.), in commercial apple orchards in Massachusetts in 2002. All traps and trap trees were baited with benzaldehyde (attractive fruit odor) plus grandisoic acid (attractive pheromone). Sticky clear Plexiglas panel traps placed at orchard borders, designed to intercept adults immigrating from border areas by flight, captured significantly more adults than similarly placed black pyramid traps, which are designed to capture adults immigrating primarily by crawling, or Circle traps wrapped around trunks of perimeter-row trees, which are designed to intercept adults crawling up tree trunks. None of these trap types, however, exhibited amounts of captures that correlated significantly with either weekly or season-long amounts of fresh ovipositional injury to fruit by adults. Hence, none appears to offer high promise as a tool for effectively monitoring the seasonal course of plum curculio injury to apples in commercial orchards in Massachusetts. In contrast, baiting branches of selected perimeter-row trees with benzaldehyde plus grandisoic acid led to significant aggregation (14-15-fold) of ovipositional injury, markedly facilitating monitoring of the seasonal course of injury to apples. A concurrent experiment revealed that addition of other synthetic fruit odor attractants to apple trees baited with benzaldehyde plus grandisoic acid did not enhance aggregation of ovipositional injury above that of this dual combination. We conclude that monitoring apples on odor-baited trap trees for fresh ovipositional injury could be a useful new approach for determining need and timing of insecticide application against plum curculio in commercial orchards.

  12. Diversidade de Carabidae (Coleoptera) Amostrados em Áreas de Reflorestamento de Mata Ciliar e Fragmento Florestal, no Estado do Paraná

    OpenAIRE

    Tamara Quinteiro; José Lopes; Ivan Martins

    2012-01-01

    Carabidae são Coleoptera com a maioria de suas espécies apresentando hábito alimentar predatório. O objetivo do presente trabalho foi estudar a diversidade e abundância da família Carabidae (Coleoptera) em áreas de fragmento florestal e em áreas de reflorestamento de mata ciliar, evidenciando a importância deste grupo como indicador da biodiversidade em relação ao sucesso do reflorestamento. Os besouros foram amostrados por meio de armadilhas de solo pitfall, em fragmentos florestais e reflor...

  13. Uncommon formation of two antiparallel sperm bundles per cyst in tenebrionid beetles (Coleoptera)

    Science.gov (United States)

    Dias, Glenda; Yotoko, Karla S. C.; Gomes, Luiz F.; Lino-Neto, José

    2012-09-01

    Several species of Tenebrionidae are stored-grain pests. Since they belong to a specious family, the systematics of these beetles is still in doubt. In insects, spermatogenesis and the spermatozoa exhibit great diversity, and are therefore commonly used in phylogenetic and taxonomic analyses. During the spermatogenetic process in these organisms, the cells originating from a single spermatogonium develop synchronically in groups referred to as cysts. At the end of this process, there is usually only one sperm bundle per cyst, with all the cells in the same orientation. This paper details the spermiogenesis of the tenebrionid beetles Tenebrio molitor, Zophobas confusa, Tribolium castaneum and Palembus dermestoides using whole mount and histological sections of the cysts. In these species, spermatogenesis is similar to that which occurs in most insects. However, during spermiogenesis, the nuclei of the spermatids migrate to two opposite regions at the periphery of the cyst, leading to the uncommon formation of two bundles of spermatozoa per cyst. This feature is possibly an apomorphy for Tenebrionidae.

  14. Locomotor activity of Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae on Chilean sandy beaches Actividad locomotora de Phalerisida maculata Kulzer (Coleoptera, enebrionidae en playas arenosas chilenas

    Directory of Open Access Journals (Sweden)

    EDUARDO JARAMILLO

    2000-03-01

    Full Text Available The locomotor activity of the beetle Phalerisida maculata Kulzer (Coleoptera Tenebrionidae was studied on the surface of the substrate in two sandy beaches of the Chilean coast: one in north central (ca. 29ºS and the other in south central Chile (ca.39ºS. During the summer period of 1991 the circadian locomotor activity was studied in the southern beach, while during that of 1997 in both beaches. To analyze the activity, pitfall traps were used which were ordered along two transects extended between the upper beach and the resurgence zone. The traps were checked (i.e. collection of captured insects every two hours for a total period of 26 hours. The results showed that the adults of P. maculata were mostly active during the night hours, whereas the larvae were active during both, the day and night. Studies carried out in the beach located in south central Chile show that differences in the tidal range (neap vs. spring tides do not affect the activity patterns. During the locomotor activity, adult and larvae move to lower intertidal levels than those usually occupied while buried. Results of laboratory experiments using actographs under conditions of darkness and constant temperature, suggest that adults and larvae of P. maculata presented a circadian rhythm similar to that observed in the field experiments. It is concluded that P. maculata presents a behaviour that appear to be under control of an endogenous rhythm, without showing differences in the circadian rhythm of activity when beaches located at different latitudes are comparedSe estudió la actividad locomotriz del escarabajo Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae sobre la superficie del sustrato de dos playas arenosas de la costa de Chile: una en el centro norte (ca. 29ºS y otra en el centro sur (ca. 39ºS. Durante el período estival de 1991 se estudió la actividad locomotriz circadiana en la playa del centro sur y durante el de 1997 en ambas playas. Para analizar

  15. The Influences of the Stress Distribution on the Condylar Cartilage Surface by Herbst Appliance Under Various Bite Reconstruction ——A Three Dimensional Finite Element Analysis%Herbst矫治器在不同重建时对髁突软骨 表面应力分布的影响

    Institute of Scientific and Technical Information of China (English)

    赵志河; 宋锦; 陈君楷; 胡林华; 樊瑜波; 蒋文涛

    2001-01-01

    Objective:The distribution of stress on the surface of condylar cartilage was investigated. Methods: Three-dimensional model of the 'Temporomandibular joint mandible Herbst appliance system' was set up by SUPER SAP software (version 9.3). On this model, various bite reconstruction was simulated according to specified advanced displacement and vertical bite opening. The distribution of maximum and minimum principal stress on the surface of condylar cartilage were computerized and analyzed.Results: When Herbst appliance drove the mandible forward, the anterior condyle surface was compressed while the posterior surface was drawn. The trend of stress on the same point on the condyle surface was consistent in various reconstruction conditions, but the trend of stress on various point were different in same reconstruction conditions. Conclusion: All five groups of bite reconstruction (3~7mm advancement, 4~2mm vertical bite opening of the mandible) designed by this study can be selected in clinic according to the patient's capability of adaptation, the extent of malocclusion and the potential and direction of growth.%目的:从生物力学角度研究Herbst矫治器在不同的重建状态下前导下颌,对髁突软骨表面应力分布的影响,为临床应用提供理论依据。方法:采用三维有限元方法,在已建立的颞下颌关节—下颌骨—Herbst矫治器系统的三维正交各向同性有限元模型上,根据临床和相关文献设计重建的类型,在髁突的矢状向及冠状向上分别选取相应节点,计算相应的最大主应力与最小主应力值。结果:不同重建时,髁突软骨表面同一部位的应力值相差不大,在相同重建时,髁突软骨表面不同部位的应力值相差较大。在每一种重建组合中,矢状向髁突软骨后方有较大范围的张应力区,而髁突前斜面有较大范围的压应力区。冠状向髁突软骨表面的内、外侧斜面都主要为

  16. Tipos de Curculionidae (Coleoptera: Curculionoidea) depositados en la colección entomológica del Museo de La Plata Types of Curculionidae (Coleoptera: Curculionoidea) housed at the Museo de La Plata entomological collection

    OpenAIRE

    Analía A. Lanteri; Suárez, Sonia M.; Guadalupe del Río, M.

    2003-01-01

    Esta contribución referida al material tipo de Curculionidae (Coleoptera: Curculionoidea) depositado en la colección entomológica del Museo de La Plata, actualiza la información sobre los tipos depositados durante los últimos diez años. Los 111 tipos examinados en este trabajo corresponden a 52 especies asignadas a las siguientes subfamilias: Curculioninae (5 spp.), Cryptorhynchinae (8 spp.), Cyclominae (4 spp.), Entiminae (31 spp.), y Erirhininae (4 spp.). Los autores de las especies son C. ...

  17. A new species of oobius trjapitzin (hymenoptera:encyrtidae) from the russian far east that parasitizes eggs of emerald ash borer (coleoptera:buprestidae)

    Science.gov (United States)

    A new egg parasitoid of the emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) from the Russian Far East, Oobius primorskyensis Yao et Duan is described. Both morphological characters and analysis of DNA sequence divergence suggest that this species is different from the previ...

  18. 伊朗北部Golestan省天牛科(鞘翅目)昆虫种类%The species of Cerambycidae (Coleoptera) from Golestan province, northern Iran

    Institute of Scientific and Technical Information of China (English)

    Najmeh SAMIN; Hamid SAKENIN

    2015-01-01

    天牛(鞘翅目Coleoptera:天牛科Cerambycidae)是树木和灌木最重要的害虫.本文记录了伊朗北部Golestan省的天牛种类,总计采集和鉴定了5亚科(Aseminae,Cerambycinae,Lamiinae,Lepturinae和Prioninae)30种天牛.

  19. Effects of exposure to agricultural drainage ditch water on survivorship, distribution, and abundnance of riffle beetles (Coleoptera: Elmidae) in headwater streams of the Cedar Creek watershed, Indiana

    Science.gov (United States)

    Riffle Beetles (Coleoptera: Elmidae) require very good water quality, mature streams with riffle habitat, and high dissolved oxygen content. As such, they prove to be good indicators of ecological health in agricultural headwater streams. We conducted static renewal aquatic bioassays using water fro...

  20. Assembly and annotation of full mitochondrial genomes for the corn rootworm species, Diabrotica virgifera virgifera and D. barberi (Insecta: Coleoptera: Chrysomelidae), using Next Generation Sequence data

    Science.gov (United States)

    Complete mitochondrial genomes for two corn rootworm species, Diabrotica v. virgifera (16,747 bp) and D. barberi (16,632; Insecta: Coleoptera: Chrysomelidae), were assembled from Illumina HiSeq2000 read data. Annotation indicated that the order and orientation of 13 protein coding genes (PCGs), and...

  1. Descrição da larva de último instar e pupa de Epacroplon cruciatum (Aurivillius (Coleoptera, Cerambycidae, Cerambycinae e notas biológica

    Directory of Open Access Journals (Sweden)

    Juares Fuhrmann

    2012-03-01

    Full Text Available Descrição da larva de último instar e pupa de Epacroplon cruciatum (Aurivillius (Coleoptera, Cerambycidae, Cerambycinae e notas biológicas. Último instar larval e pupa do Hexoplonini sul-americano Epacroplon cruciatum (Aurivillius, 1899 são descritos, ilustrados e disponibilizadas notas biológicas.

  2. Immunofluorescence localization and ultrastructure of Stewart’s wilt disease bacterium Pantoea stewartii in maize leaves and in its flea beetle vector Chaetocnema pulicaria (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Pantoea stewartii is the causal agent of Stewart's wilt of sweet corn, the most serious bacterial disease of sweet corn and maize in the North-Central and Eastern USA. P. stewartii is transmitted mainly by the corn flea beetle Chaetocnema pulicaria (Coleoptera: Chrysomelidae) and this bacterium is a...

  3. Brood production by Xylosandrus germanus (Coleoptera: Curculionidae) and growth of its fungal symbiont on artificial diet based on sawdust of different species

    Science.gov (United States)

    The ambrosia beetle Xylosandrus germanus (Blanford) (Coleoptera: Curculionidae) is among the most important exotic pests of orchards and nurseries in the US. It attacks a wide range of hosts and is difficult to control using conventional insecticides. As part of our studies on the biology and cont...

  4. Trapping sweetpotato weevil, Cylas formicarius elegantulus (Coleoptera: Brentidae), with high doses of sex pheromone: Catch enhancement and weathering rate in Hawaii

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lamarck, one of the top ten staple crops produced worldwide, has increased in production in Hawaii in recent years. The sweetpotato weevil, Cylas formicarius elegantulus (Summers)(Coleoptera: Brentidae), is a major economic and quarantine pest of sweetpotato in Hawa...

  5. Post entry interception of the yellow-spotted longhorned beetle,Psacothea hilaris (Coleoptera: Cerambycidae) in Italy Genbank Accession Number GU244486

    Science.gov (United States)

    Psacothea hilaris (Pascoe) (Coleoptera: Cerambycidae) known as the yellow-spotted longhorned beetle is native from eastern Asia (China, Japan including Ryukyu, Shikoku and Honshu archipelago and Taïwan) where it attacks plants belonging to Moraceae family, in particular to Morus and Ficus genera. In...

  6. Biology of two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae), recently invasive in the U.S.A., reared on an ambrosia beetle artificial diet

    Science.gov (United States)

    1. Diet and rearing protocols were developed for two members of the cryptic Euwallacea fornicatus species complex, polyphagous shot hole borer (PSHB) and tea shot hole borer (TSHB) (Coleoptera: Curculionidae: Scolytinae), using sawdust from boxelder Acer negundo and avocado Persea americana. 2. Bio...

  7. New records of Paracrias Ashmead (Hymenoptera, Eulophidae as parasitoids on weevil larvae (Coleoptera, Curculionidae in Brazil, with the description of a new species

    Directory of Open Access Journals (Sweden)

    Luciano Palmieri

    2013-09-01

    Full Text Available Paracrias strii Schauff, 1985 and P. ceratophaga Palmieri & Hansson sp. nov. are first record in Brazil and both are associated with Ceratopus Schoenherr larvae (Coleoptera, Curculionidae reared from syconia of two species of fig-trees. Both Paracrias species are diagnosed and illustrated. Males of P. ceratophaga sp. nov. are described. The association of Paracrias with weevil larvae is briefly discussed.

  8. Current Knowledge of Mesozoic Coleoptera from Daohugou and Liaoning (Northeast China)

    Institute of Scientific and Technical Information of China (English)

    Alexander G.KIREJTSHUK; Alexander G.PONOMARENKO; Alexander A.PROKIN; CHANG Huali; Georgy V.NIKOLAJEV; REN Dang

    2010-01-01

    The present paper is devoted to an overview on fossil Coleoptera studied from Inner Mongolia,Daohugou (Middle Jurassic,Jiulongshan Formation) and Liaoning (Upper Jurassic-Lower Cretaceous,Yixian Formation) deposited in Chinese collections.As a result,species of the tribe Sperchopsini and Hydrophilini from Hydrophilidae,families and subfamilies Silphidae,Syndesinae from Lucanidae,Pleocomidae,Trogidae,Trogissitidae,Pyrochroidae,Diaperinae from Tenebrionidae,and Cerambycidae were first registered in the Mesozoic and some families were defined as new.It was shown that many superfamilies represented in the Recent Fauna were formed within the Middle Jurassic and Lower Cretaceous.The materials examined confirm the hypothesis that Cucujiformian beetles are a younger group than other infraordera of Polyphaga (Staphyliniformia and Elateriformia) and,therefore,they appeared in the fossil record only in the late Mesozoic.It was shown and confirmed that most superfamilies appeared in the fossil records before Cucujoidea.The synonymy of Notocupes Ponomarenko,1964;Sinocupes Lin,1976,syn.nov.;Amblomma Tan,Ren et Liu 2005,syn.nov.;Euryomma Tan,Ren et Shih,2006,syn.nov.,non Stein,1899 and Ovatocupes Tan et Ren,2006,syn.nov.;synonymy of Tetraphalerus Waterhouse,1901 and Odontomma Tan,Ren et Ge 2006,syn.nov.;and synonymy of Priacmopsis Ponomarenko,1966 and Latocupes Tan et Ren,2006,syn.nov.are proposed.Sinorhombocoleus papposus Tan et Ren,2009 is transferred from the family Rhombocoleidae to Schizophoridae.Cervicatinius complanus Tan,Ren et Shih,2007 and Forticatinius elegans Tan,Ren et Shih,2007 are transferred from the family Catiniidae (suborder Archostemata) to superfamily Cleroidea (suborder Polyphaga:first among the family Peltidae and second as a closely related group to the latter family).The family Parandrexidae is transferred from the superfamily Cucujoidea to Cieroidea.The ecological circumstances of the past ecosystems and hypotheses of historical development of the order

  9. Avaliação de inseticidas no controle de Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae e Rhyzopertha dominica (Fabricius (Coleoptera, Bostrichidae em trigo armazenado Efficacy of insecticides to control Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae and Rhyzopertha dominica (Fabricius (Coleoptera, Bostrichidae in stored wheat

    Directory of Open Access Journals (Sweden)

    Rui S. Furiatti

    1999-03-01

    Full Text Available The efficiency of insecticides against Sitophilus oryzae (Linnaeus, 1763 (Coleoptera, Curculionidae and Rhyzopertha dominica (Fabricius, 1792 (Coleoptera, Bostrichidae was determined exposing them to treated wheat in laboratory. Each plot, which was composed by 1.2 kg of wheat grain, was treated with 5 ml of the concentration/kg of wheat using a propelling air atomizer. Fifteen days after treatment 50 g samples of the grain were taken, from each plot, placed in plastic jars into which 10 adult insects of each species were released. This was repeated every 30 days until 165 days after treatment. The number of dead insects was counted 15 days after each infestation thus the evaluations were made at 30, 60, 90, 120, 150 and 180 days after the insecticide application. Esfenvalerate+fenitrothion+pyperonil butoxide (0,375+7,5+3,0 and 0,5+10,0+4,0 g a.i./t, fenitrothion+esfen-valerate (7,5+0,375 and 10,0+0,375 g a.i./t, fenitrothion+synergised deltamethrin (7,5+0,375 g a.i./t and fenitrothion (7,5 g a.i./t were effective against Sitophilus oryzae until 180 days after treatment. Esfenvalerate+fenitrothion+pyperonil butoxide (0,5+10,0+4,0 g a.i./t, fenitrothion+esfenvalerate (7,5+0,375 and 10,0+0,375 g a.i./t, fenitrothion+synergised deltamethrin (7,5+0,375 g a.i./t were effective against R. dominica until 180 days after treatment. Synergised deltamethrin (0,375 g a.i./t was effective against S. oryzae until 150 days and against R. dominica until 120 days after treatment. Esfenvalerate (0,5 g a.i./t was ineffective against S. oryzae and effective until 60 days after treatment against R. dominica. Fenitrothion (7,5 g a.i./t was ineffective against R. dominica. The use of mixtures of organophosphorus plus pyrethroids were more effective against S. oryzae and R. dominica when compared with these insecticides used alone.

  10. Coleoptera and microbe biomass in Antarctic Dry Valley paleosols adjacent to the Inland Ice: Implications for Mars

    Science.gov (United States)

    Mahaney, William C.; Hart, Kris M.; O'Reilly, Shane S.; Allen, Christopher C. R.; Dohm, James M.; Hancock, Ronald G. V.; Kelleher, Brian P.; Milner, Michael W.

    2012-01-01

    Bulk paleosol samples collected from a Middle to Early Miocene moraine in the New Mountain area of the Dry Valleys, Antarctica, yielded Coleoptera exoskeletons and occasional endoskeletons showing considerable diagenetic effects along with several species of bacteria, all lodged in a dry-frozen but salt-rich horizon at shallow depth to the land surface. The till is at the older end of a chronologic sequence of glacial deposits, thought to have been deposited before the transition from wet-based to cold-based ice (∼15 Ma), and hence, entirely weathered in contact with the subaerial atmosphere. It is possible, though not absolutely verifiable, that the skeletons date from this early stage of emplacement having undergone modifications whenever light snowmelt occurred or salt concentrations lowered the freezing temperature to maintain water as liquid. Correlation of the Coleoptera species with cultured bacteria in the sample and the likelihood of co-habitation with Beauveria bassiani found in two adjacent, although younger paleosols, leads to new questions about the antiquity of the Coleoptera and the source of N and glucose from chitinase derived from the insects. The skeletons in the 831 section may date close to the oldest preserved chitin (Oligocene) yet found on Earth. While harsh Martian conditions make it seemingly intolerable for complex, multicellular organisms such as insects to exist in the near-surface and subaerially, life within similar cold, dry paleosol microenvironments (Cryosols) of Antarctica point to life potential for the Red Planet, especially when considering the relatively diverse microbe (bacteria and fungi) population.

  11. Seasonal dynamics of saproxylic beetles (Coleoptera occurring in decaying birch (Betula spp. wood in the Kampinos National Park

    Directory of Open Access Journals (Sweden)

    Sawoniewicz Michał

    2015-09-01

    Full Text Available The aim of the study was to identify the seasonal changes in the number of saproxylic beetles connected with birch in the Kampinos National Park. The research was conducted for 12 consecutive months in research areas representing 10 different site types. The beetles were collected from wood using photoeclectors. The largest number of species was collected in April and the lowest in January. An increase in number occurred during spring and summer months for species associated only with rotting wood, fructifications of tree fungi, the subcortical environment and hollows. In the same period the number of species not associated or potentially associated with decaying trees and wood decreased. During winter months, the differences in the number of trapped specimens were the smallest. The proportion of zoophagous species amongst the collected specimen increased in autumn and winter. The share of saprophagous species was the highest during the summer-autumn period and the share of mycetophages (jointly with myxomycophages was the highest during spring and summer. We distinguished two separate groups of Coleoptera with the first one (‘summer group’ including species trapped during late-spring and summer months, while the second one (‘winter group’ includes species found in autumn, winter and early-spring months. In the ‘summer group’, an average of 55.8 species was trapped each month with 331.2 specimen of Coleoptera, while in the ʻwinter group’ an average of 56.1 species with 228.4 Coleoptera specimen were caught.

  12. Redefinition of the genus Silphitrombium (Trombidiformes: Neothrombiidae) with description of two new species parasitizing beetles (Coleoptera: Elateridae, Tenebrionidae) from Iran.

    Science.gov (United States)

    Tashakor, Samaneh; Hajiqanbar, Hamidreza; Saboori, Alireza

    2013-11-15

    Two new species of Silphitrombium Fain, 1992 (Acari: Prostigmata: Neothrombiidae), ectoparasites of beetles (Insecta: Coleoptera), are described from Sistan and Baluchestan Province, eastern Iran: S. elateridum sp. nov. on Heteroderes heideni Reitter, 1891 (Col.: Elateridae) and S. iranicum sp. nov. on Opatroides punctulatus Brullé, 1832 (Col.: Tenebrionidae) and the genus Silphitrombium is redefined. It is the first record of the relationship between beetles of the families Elateridae and Tenebrionidae, and mites of the genus Silphitrombium. A key to the species of the genus is presented.

  13. Sucinolivolia torpida--a new genus and species of flea-beetles (Coleoptera: Chrysomelidae: Galerucinae) from Baltic amber.

    Science.gov (United States)

    Bukejs, Andris; Biondi, Maurizio; Alekseev, Vitalii I

    2015-12-15

    Sucinolivolia torpida gen. nov. et sp. nov. (Coleoptera: Chrysomelidae: Galerucinae: Alticini) is described and illustrated from Eocene Baltic amber. The new monotypic genus is compared with fossil and extant flea-beetle genera. Sucinolivolia gen. nov. is similar to the extant Livolia Jacoby and Orthaltica Crotch, but difference include the absence of an antebasal pronotal sulcus, not crenulate lateral pronotal margins, possessing very short genae, more robust legs, and the shape of tibiae. Including this new record, six described species of Alticini are known from Baltic amber.

  14. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    Abstract A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  15. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus.

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  16. Effect of temperature on reproduction and embryonic development of the cabbage stem flea beetle, Psylliodes chrysocephala L., (Coleoptera: Chrysomelidae)

    DEFF Research Database (Denmark)

    Mathiasen, Helle; Sørensen, Helle; Bligaard, J.;

    2015-01-01

    The cabbage stem flea beetle, Psylliodes chrysocephala (L.) (Coleoptera: Chrysomelidae), is a major pest of winter oilseed rape. Despite the importance of this pest, detailed information on reproduction to predict risk of crop damage is lacking. This study investigates the effect of temperature...... on parameters of reproduction, egg development and viability at five constant temperatures. Significant temperature effects were found on the pre-oviposition period, total number of eggs laid, daily oviposition rate, female longevity, egg-development rate and viability. The mean length of the pre...

  17. Influence of 60Co γ irradiation on fertility of Japanese pine sawyer beetle Monochamus alternatus hope (Coleoptera: Cerambycidae)

    International Nuclear Information System (INIS)

    The fertility of the Japanese pine sawyer beetle Monochamus alternatus (Coleoptera: Cerambycidae) irradiated with 60Co γ-rays was remarkable reduced at the doses of 30Gy, 35Gy and 40Gy, especially at 40Gy. When the non-irradiated females were coupled with the irradiated males first, and then coupled with non-irradiated males, the hatchability and the fertility had little higher but lower than the control. It explained that radiation has certain influence to the female gonad. It also has difference between the hatching rate and the amount of eggs in different match. (authors)

  18. Registros y distribución de la especie afroasiática Digitonthophagus gazella (Coleoptera: Scarabaeidae: Scarabaeinae en Argentina

    Directory of Open Access Journals (Sweden)

    María C. ÁLVAREZ BOHLE

    2009-01-01

    Full Text Available Se cita por primera vez el escarabajo coprófago Digitonthophagus gazella Fabricius (Coleoptera: Scarabaeidae para Argentina, por lo que se amplía su área de distribución geográfica en Sudamérica. Esta especie de origen afro-asiático fue capturada mediante colecta manual en estiércol de ganado bovino y equino, con trampas de luz y trampas de caída cebadas con estiércol en seis provincias del centro y norte argentino entre los años 2006 y 2009.

  19. Diversity and seasonality of Scarabaeinae (Coleoptera: Scarabaeidae in forest fragments in Santa Maria, Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    PEDRO G. DA SILVA

    2013-05-01

    Full Text Available Scarabaeinae specimens were collected with the use of pitfall traps baited with human excrement, rotten meat and rotting banana, between May 2009 and April 2010, in three forest fragments in Santa Maria, Rio Grande do Sul, Brazil: ‘Morro do Elefante' (MOEL, ‘Morro do Cerrito' (MOCE and ‘Campus da Universidade Federal de Santa Maria' (UFSM. A total of 19,699 individuals belonging to 33 species were collected. Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. and Canthon lividus Blanchard, 1845, were the most abundant species. MOEL showed the greatest richness, MOCE the greatest abundance, while UFSM showed the lowest values of richness and abundance. The greatest similarity (qualitative and quantitative was found between MOEL and MOCE, while the lowest occurred between MOCE and UFSM. Only 51% of the species were common to all three fragments. The richness and abundance of Scarabaeinae were positively correlated with the air temperature. The richness of the fragment decreased according to the smaller size and the greater degree of disturbance of vegetation structure.Espécimes de Scarabaeinae foram coletados com o uso de armadilhas de queda iscadas com excremento humano, carne apodrecida e banana em decomposição, entre maio de 2009 e abril de 2010, em três fragmentos florestais em Santa Maria, Rio Grande do Sul, Brasil: Morro do Elefante (MOEL, Morro do Cerrito (MOCE e Campus da Universidade Federal de Santa Maria (UFSM. Um total de 19.699 indivíduos de 33 espécies foi coletado; Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. e Canthon lividus Blanchard, 1845 foram as espécies mais abundantes. MOEL apresentou a maior riqueza, MOCE a maior abundância, enquanto UFSM teve os menores valores de riqueza e abund

  20. Diversity and seasonality of Scarabaeinae (Coleoptera: Scarabaeidae in forest fragments in Santa Maria, Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    PEDRO G. DA SILVA

    2013-06-01

    Full Text Available Scarabaeinae specimens were collected with the use of pitfall traps baited with human excrement, rotten meat and rotting banana, between May 2009 and April 2010, in three forest fragments in Santa Maria, Rio Grande do Sul, Brazil: ‘Morro do Elefante’ (MOEL, ‘Morro do Cerrito’ (MOCE and ‘Campus da Universidade Federal de Santa Maria’ (UFSM. A total of 19,699 individuals belonging to 33 species were collected. Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. and Canthon lividus Blanchard, 1845, were the most abundant species. MOEL showed the greatest richness, MOCE the greatest abundance, while UFSM showed the lowest values of richness and abundance. The greatest similarity (qualitative and quantitative was found between MOEL and MOCE, while the lowest occurred between MOCE and UFSM. Only 51% of the species were common to all three fragments. The richness and abundance of Scarabaeinae were positively correlated with the air temperature. The richness of the fragment decreased according to the smaller size and the greater degree of disturbance of vegetation structure.Espécimes de Scarabaeinae foram coletados com o uso de armadilhas de queda iscadas com excremento humano, carne apodrecida e banana em decomposição, entre maio de 2009 e abril de 2010, em três fragmentos florestais em Santa Maria, Rio Grande do Sul, Brasil: Morro do Elefante (MOEL, Morro do Cerrito (MOCE e Campus da Universidade Federal de Santa Maria (UFSM. Um total de 19.699 indivíduos de 33 espécies foi coletado; Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. e Canthon lividus Blanchard, 1845 foram as espécies mais abundantes. MOEL apresentou a maior riqueza, MOCE a maior abundância, enquanto UFSM teve os menores valores de riqueza e

  1. Ambrosia Beetle (Coleoptera: Scolytidae) Species, Flight, and Attack on Living Eastern Cottonwood Trees.

    Energy Technology Data Exchange (ETDEWEB)

    Coyle, D R; D.C. Booth: M.S. Wallace

    2005-12-01

    ABSTRACT In spring 2002, ambrosia beetles (Coleoptera: Scolytidae) infested an intensively managed 22-ha tree plantation on the upper coastal plain of South Carolina. Nearly 3,500 scolytids representing 28 species were captured in ethanol-baited traps from 18 June 2002 to 18 April 2004. More than 88% of total captures were exotic species. Five species [Dryoxylon onoharaensum (Murayama), Euwallacea validus (Eichhoff), Pseudopityophthorus minutissimus (Zimmermann), Xyleborus atratus Eichhoff, and Xyleborus impressus Eichhoff]) were collected in South Carolina for the first time. Of four tree species in the plantation, eastern cottonwood, Populus deltoides Bartram, was the only one attacked, with nearly 40% of the trees sustaining ambrosia beetle damage. Clone ST66 sustained more damage than clone S7C15. ST66 trees receiving fertilization were attacked more frequently than trees receiving irrigation, irrigation_fertilization, or controls, although the number of S7C15 trees attacked did not differ among treatments. The study location is near major shipping ports; our results demonstrate the necessity for intensive monitoring programs to determine the arrival, spread, ecology, and impact of exotic scolytids.

  2. ATIVIDADE INSETICIDA DE PLANTAS MEDICINAIS SOBRE O Callosobruchus maculatus (COLEOPTERA: BRUCHIDAE

    Directory of Open Access Journals (Sweden)

    Antonia Mirian Nogueira de Moura Guerra

    2009-01-01

    Full Text Available This study aimed to evaluate the insecticidal activity of eight medicinal plants on Callosobruchus maculatus (F. (Coleoptera: Bruchidae. Plant powder from Rosmarinus officinalis (L. leaves, Peumus boldus (Mol leaves, Matricaria chamomilla (L. flowers, Baccharis trimera (Less. leaves, Camellia sinensis (L. leaves, Thea sinensis (L. leaves, Ilex paraguariensis (St. Hil. leaves, and fruits of Pimpinella anisum (L. were used in the experiment. Bioassays were carried out under constant conditions of temperature (28±2 °C, relative humidity (70±5% and scotophase of 24 hours. Experimental units consisted of 20 grams of cowpea (Vigna unguiculata (L. Walp. grains with 13% wet basis moisture content, 2 g plant powder and ten adult insects, both placed in plastic containers (60 mm in diameter x 60 mm high. The analyzed variables included: insect mortality, oviposition (egg number and instantaneous rate of population growth (ri. The number of dead insects was recorded 5 days after bioassays were started, the egg number was recorded after 12 days, and ri was estimated on the 60th day. All the tested plants had toxicity to C. maculatus. The highest mortality was found in insects treated with P. boldus and I. paraguariensis. Number of eggs and ri also varied significantly among the powders. The lowest mean values for these parameters were recorded in the plots treated with P. boldus, P. anisum and R. officinalis.

  3. Fumigation Toxicity of Essential Oil Monoterpenes to Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae

    Directory of Open Access Journals (Sweden)

    Olufunmilayo E. Ajayi

    2014-01-01

    Full Text Available The fumigant toxicity of eight essential oil components, 1-8-cineole, carvacrol, eugenol, (−-menthone, (−-linalool, S-(−-limonene, (−-β-pinene, and (+-α-pinene, was tested against the cowpea weevil, Callosobruchus maculatus (Fabricius (Coleoptera: Chrysomelidae, at 0.25–60 µL/L air doses. 1-8-Cineole, carvacrol, and eugenol caused complete adult mortality at 10 µL/L air 24 h after treatment. 1-8-Cineole and carvacrol were the most toxic with LD50 values of 0.24 and 0.6 µL/L air at 24 h, respectively. (−-β-Pinene and (+-α-pinene were the least toxic with LD50 values of 31 and 31.4 µL/L air at 24 h, respectively. Toxicity was negatively correlated with vapor pressure. 1-8-Cineole and carvacrol caused 100% oviposition deterrence at all doses tested. Eugenol and (−-menthone completely inhibited adult emergence. S-(−-Limonene, (−-β-pinene, and (+-α-pinene were not effective at preventing oviposition or adult emergence, suggesting that a lethal dose of the three oil components would be necessary to control C. maculatus infestations.

  4. Sustainable management tactics for control of Phyllotreta cruciferae (Coleoptera: Chrysomelidae) on canola in Montana.

    Science.gov (United States)

    Reddy, Gadi V P; Tangtrakulwanich, Khanobporn; Miller, John H; Ophus, Victoria L; Prewett, Julie

    2014-04-01

    The crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae), has recently emerged as a serious pest of canola (Brassica napus L.) in Montana. The adult beetles feed on canola leaves, causing many small holes that stunt growth and reduce yield. In 2013, damage to canola seedlings was high (approximately 80%) in many parts of Montana, evidence that when flea beetles emerge in large numbers, they can quickly destroy a young canola crop. In the current study, the effectiveness of several biopesticides was evaluated and compared with two insecticides (deltamethrin and bifenthrin) commonly used as foliar sprays as well as seed treatment with an imidacloprid insecticide for the control of P. cruciferae under field conditions in 2013. The biopesticides used included an entomopathogenic nematode (Steinernema carpocapsae), two entomopathogenic fungi (Beauveria bassiana and Metarhizium brunneum), neem, and petroleum spray oils. The control agents were delivered in combination or alone in a single or repeated applications at different times. The plant-derived compound neem (azadirachtin), petroleum spray oil, and fatty acids (M-Pede) only showed moderate effect, although they significantly reduced leaf injuries caused by P. cruciferae and resulted in higher canola yield than the untreated control. Combined use of B. bassiana and M. brunneum in two repeated applications and bifenthrin in five applications were most effective in reducing feeding injuries and improving yield levels at both trial locations. This indicates that entomopathogenic fungi are effective against P. cruciferae, and may serve as alternatives to conventional insecticides or seed treatments in managing this pest. PMID:24772547

  5. Transcriptomic Analysis of Eucryptorrhynchus chinensis (Coleoptera: Curculionidae) Using 454 Pyrosequencing Technology.

    Science.gov (United States)

    Liu, Zhen-Kai; Wen, Jun-Bao

    2016-01-01

    Eucryptorrhynchus chinensis Olivier (Coleoptera: Curculionidae) is one of the most important pests of Ailanthus altissima; however, so far, no studies on the genome or transcriptome of E. chinensis have been reported. Using the Roche 454 FLX Titanium platform, an RNA pool obtained from E. chinensis eggs, larva, pupae, and adults was sequenced and assembled de novo to achieve maximum diversity of sampled transcripts. We obtained 1,441,137 (∼518 Mb) raw reads with an average length of 360 bp. After trimming, 89% qualified reads were produced and assembled into 35,509 isotigs with an average length of 440 bp, N50 of 1,048 bp, and 111,643 singletons. We generated 87,894 unigenes following a cluster analysis of the isotigs and singletons, and then functionally annotated the unigenes with gene descriptions. We obtained 23,363 GO assignments, and 12,724 unigenes were assigned to KOG. Based on these annotations, 294 biochemical pathways involved in growth, reproduction, and stress or immune responses were predicted. A total of 659,026 single nucleotide variants and 6,112 simple sequence repeats were detected. Our data provide comprehensive information on the sequence and possible functions of E. chinensis transcripts. PMID:27620556

  6. Annotated catalogue of the carabid beetles of the Republic of Macedonia (Coleoptera: Carabidae).

    Science.gov (United States)

    Hristovski, Slavčo; Guéorguiev, Borislav

    2015-08-20

    The catalogue of the ground beetles (Coleoptera: Carabidae) of the Republic of Macedonia is the result of our permanent investigation during 15 years. It is based on the critical review of the data in 255 scientific publications and the revision of the collections deposited in the museums in Macedonia (Skopje and Struga), other European countries (Berlin, Budapest, Vienna, Sofia) and the first author's private collection. For all of the species and subspecies we have presented the known literature references, precise data for the studied material and overall distribution in the Republic of Macedonia. The study of the material resulted in new country records of 10 genera, 101 species and 25 subspecies. First detailed records are provided for another 47 species and subspecies, and additional material was studied of 482 species and subspecies. Type material of 18 species and subspecies was also examined. Thirteen species and one subspecies were rejected from the list of Macedonian ground beetles. Six more species are treated as questionable and were not included in the present list. As a result, the presence of 571 species and 234 subspecies (626 taxa in total) in Macedonia is confirmed. These taxa are arranged in 104 genera, 31 subtribes, 35 tribes and 13 subfamilies. The most numerous in term of the species are the genera Bembidion (60), Harpalus (48) and Amara (46), as well as Pterostichus (26), Ophonus (19), Carabus (16), Trechus (16), Brachinus (16) and Dyschirius (15).

  7. Treatment outcome of Paederus dermatitis due to rove beetles (Coleoptera: Staphylinidae) on guinea pigs.

    Science.gov (United States)

    Fakoorziba, M R; Eghbal, F; Azizi, K; Moemenbellah-Fard, M D

    2011-08-01

    Linear dermatitis (or dermatitis linearis, DL) is a skin blistering inflammatory lesion caused by exposure to the pederin toxin from rove beetles. Although it is prevalent in many countries of the Middle East region, this is not a notifiable disease. In recent years, a number of clinical symptoms outbreaks of DL has been reported from a few neighboring countries of Iran, but no report of experimental treatment among small laboratory rodents is known. This is a prerequisite to ascertain the nature of the best treatment strategy in cases of infestation with these beetles, as it occurs among local settlers during hot seasons in certain parts of the southern Iranian province of Fars. Live Paederus beetles were collected, identified to species level, sexed apart and partly processed to obtain their hemolymph toxin pederin in ethanol for dermal application on guinea pigs. Two Paederus species were found. Paederus ilsae (Bernhauer) (Coleoptera: Staphylinidae) was more abundant than P. iliensis (Coiffait). Recovery from DL due to live P. ilsae beetles was quicker and less complex than that of pederin in ethanol on guinea pigs. The application of potassium permanganate with calamine to heal DL was also more effective than fluocinolone treatment. This topical corticosteroid is thus considered less able to avert the cytotoxic action of pederin on the skin of guinea pigs than the antipruritic and cleansing agents. It seems likely that fluocinolone has certain effects which delays the recovery period for the treated skin.

  8. Stenusine, an antimicrobial agent in the rove beetle genus Stenus (Coleoptera, Staphylinidae)

    Science.gov (United States)

    Lusebrink, Inka; Dettner, Konrad; Seifert, Karlheinz

    2008-08-01

    Stenusine is well known as the alkaloid, discharged by the rove beetle, genus Stenus Latreille (Coleoptera, Staphylinidae). The Stenus beetles employ the alkaloid as an escape mechanism when on water surfaces. In the case of danger, they lower their abdomen and emit stenusine from their pygidial glands. Stenusine shows a low surface tension and therefore a high spreading pressure; these properties propel the beetle quickly over the water. Many Steninae do not live in habitats with open waters, but in detritus, leaf litter, mosses, etc. This raises the possibility that stenusine might also have another function, e.g., as antibiotic or fungicide. Stenus beetles show an intense grooming behaviour. With gas chromatography mass spectrometry analyses we could prove that they cover themselves with their secretion. To tests its antimicrobial properties we conducted agar diffusion tests with stenusine and norstenusine, another substance that is abundant in most Stenus species. Both compounds have an antimicrobial effect on entomopathogenic bacteria and fungi. Stenusine not only allows for an extraordinary method of locomotion on water surfaces, it also protects the Steninae from being infested with microorganisms.

  9. Identification, distribution, and adult phenology of the carrion beetles (Coleoptera: Silphidae) of Texas.

    Science.gov (United States)

    Mullins, Patricia L; Riley, Edward G; Oswald, John D

    2013-01-01

    The carrion beetles (Coleoptera: Silphidae) of Texas are surveyed. Thirteen of the 14 species, and five of the six genera, of this ecologically and forensically important group of scavengers that have previously been reported from Texas are confirmed here based on a study of 3,732 adult specimens. The one reported, but unconfirmed, species, Oxelytrum discicolle, was probably based on erroneous label data and is excluded from the Texas fauna. Two additional species, Nicrophorus sayi and N. investigator are discussed as possible, but unconfirmed, components of the fauna. Taxonomic diagnoses, Texas distribution range maps, seasonality profiles, and biological notes are presented for each confirmed species. The confirmed Texas silphid fauna of 13 species comprises 43% of the 30 species of this family that are known from America north of Mexico. The highest richness (11 species) is found in the combined Austroriparian and Texan biotic provinces of eastern Texas. Phenologically, three species (Necrophila americana, O. rugulosum and Nicrophorus tomentosus) exhibit bimodal adult temporal occurrence patterns with peaks in the spring and late summer or fall; four species (Oiceoptoma noveboracense, Necrodes surinamensis, Nicrophorus carolinus, and N. orbicollis) exhibit unimodal occurrence patterns with a single peak in late spring or early summer; one species (Oiceoptonia inaequale) exhibits a unimodal occurrence pattern with a single peak in early spring; and five species (Thanatophilus truncatus, Nicrophorus americanus, N. marginatus, N. mexicanus and N. pustulatus) display unimodal occurrence patterns with peaks in late summer or early fall.

  10. Fossil mesostigmatid mites (Mesostigmata: Gamasina, Microgyniina, Uropodina), associated with longhorn beetles (Coleoptera: Cerambycidae) in Baltic amber

    Science.gov (United States)

    Dunlop, Jason A.; Kontschán, Jenő; Zwanzig, Michael

    2013-04-01

    Fossil mesostigmatid mites are extremely rare. Inclusions assignable to the tortoise mites (Mesostigmata, Uropodina) are described here for the first time from Eocene (ca. 44-49 Ma) Baltic amber. This is the oldest record of Uropodina and documents the first unequivocal amber examples potentially assignable to the extant genus Uroobovella Berlese, 1903 (Uropodoidea: Urodinychidae). Further mites in the same amber pieces are tentatively assigned to Microgynioidea (Microgyniina) and Ascidae (Gamasina), both potentially representing the oldest records of their respective superfamily and family groups. This new material also preserves behavioural ecology in the form of phoretic deutonymphs attached to their carriers via a characteristic anal pedicel. These deutonymphs in amber are intimately associated with longhorn beetles (Coleoptera: Cerambycidae), probably belonging to the extinct species Nothorhina granulicollis Zang, 1905. Modern uropodines have been recorded phoretic on species belonging to several beetle families, including records of living Uroobovella spp. occurring on longhorn beetles. Through these amber inclusions, a uropodine-cerambycid association can now be dated back to at least the Eocene.

  11. Artificial substrates for oviposition and larval development of the pepper weevil (Coleoptera: Curculionidae).

    Science.gov (United States)

    Addesso, K M; McAuslane, H J; Stansly, P A; Slansky, F; Schuster, D J

    2009-02-01

    The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae), is a major pest of cultivated peppers (Capsicum spp.) and other cultivated and wild species within the family Solanaceae. Laboratory study of this insect, as well as its biological control agents, will be greatly facilitated by an artificial rearing system that does not rely on pepper fruit. An egg collection method and amendments to a standard larval diet were investigated for use in the rearing of this weevil. Spherical sachets made of Parafilm or netting enclosing leaves of pepper, American black nightshade, eggplant, tomato, potato, and jasmine tobacco induced oviposition. Tomato, potato, and jasmine tobacco leaves were accepted despite the fact that these are not oviposition hosts for pepper weevils in the wild. A standard larval diet formula was modified in an attempt to improve egg hatch, larval survival, developmental time, and adult mass. The diet formula was modified with the addition of freeze-dried jalapeño pepper powder, an additional lipid source, alternate protein sources, and the removal of methyl paraben. None of the aforementioned treatments resulted in a significant improvement over the standard diet. Egg hatch was greater when eggs were incubated on moist paper towels rather than in diet; thus, placement of neonates rather than eggs into diet improved production of adults. Suggestions for more efficient rearing of weevils on the currently available diet and future directions for the development of an artificial rearing system for pepper weevil are discussed.

  12. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

    Science.gov (United States)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2015-06-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. PMID:26470205

  13. Preliminary Diversity Of Dung Beetles (Coleoptera: Scarabaeinae Attracting By Human Dung (Calvario, Meta

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    Ruth Mariela Castillo Morales

    2011-12-01

    Full Text Available In the Private Reserve, Refugio del Oso de Anteojos (El Calvario-Meta, the diversity of dung beetles was studied (Coleoptera: Scarabaeinae, grouped around different types of vegetation (Montane Rain Forest, Rainforest Premontane, Lower Montane Rain Forest, by using pitfall traps baited with human dung. During the samplings carried out in the rainy station on 2008, were captured a total of 177 individuals belonging to 9 species. The tribe Canthonini was the most representative with 87.5%, of the described species, followed by Aechini 10.8%, and Onthophagini 1.7%. The dominant species was Canthon lituratus in the three vegetation units: Lower montane rain forest was the richest (9 species and abundance (86 individuals, compared with other environments sampled. By calculating the complementarity index, shows a similar species composition in the three habitats sampled, possibly, related to the floristic composition presented in each one of them.

  14. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae

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    Andréa Roveré Franz

    2011-03-01

    Full Text Available Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae. Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf, Zingiber officinale (root and Mentha sp. (leaf. The efficacy of these oils was tested to control the rice weevil, S. oryzae, using hydrodistillation. Chemical analysis of the essential oils was carried out by gas chromatography. Major components of C. citratus were geranial (48% and neral (31%, of Z. officinale were α-zingibereno (13%, geranial (16%, neral (10% and α-farneseno (5% and of Mentha sp. was menthol (92%. Bioassays were carried out by fumigation and topical application. In topical application assays, the essential oil of C. citratus had greater toxicity (LC50 0.027 µL mL-1 and shorter exposure time than the oils of the other two plants. After 24 h and 48 h, 70% and 100% mortality of S. oryzae occurred, respectively. In fumigation assays, essential oil of Z. officinale had a lower LC50 (1.18 µL cm-2 and 70% mortality after 24 h exposure. Therefore, we recommend the use of essential oils of C. citratus and Z. officinale to control the rice weevil S. oryzae.

  15. Characterization of the complete mitochondrial genome of the firefly, Luciola substriata (Coleoptera: Lampyridae).

    Science.gov (United States)

    Mu, Feng-Juan; Ao, Liang; Zhao, Hua-Bin; Wang, Kai

    2016-09-01

    The firefly, Luciola substriata (Coleoptera: Lampyridae), is an aquatic firefly species, whose larvae inhabit ponds or lakes. Here we present the complete mitochondrial (mt) genome of the firefly (GenBank accession number KP313820) and provide its annotation. This circular genome is 16,248 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a non-coding AT-rich region. Similar to other firefly species, the base composition of this mitochondrial genome is also biased toward A and T (44.09% A, 34.00% T, 12.89% C, and 9.01% G). All 13 protein-coding genes start with a typical mitochondrial start codon, and terminate with a usual stop codon TAA, or TAG or a single T. The non-coding AT-rich region (1636 bp in length) include one (A)20, and two (T)15 tandem repeats, and one (AAT)5 element. This mitochondrial genome sequence will promote a better understanding for firefly evolution in the future. PMID:25714154

  16. Development and Life History of Sitophilus zeamais (Coleoptera: Curculionidae on Cereal Crops

    Directory of Open Access Journals (Sweden)

    James Adebayo Ojo

    2016-01-01

    Full Text Available The maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae, is one of the most destructive pests of stored cereals. Knowledge of the life history and biology is important to the development of an integrated pest management program. Investigation was carried out on developmental biology of S. zeamais on four main cereal crops, maize, rice, sorghum, and millet, under laboratory conditions. Egg incubation, oviposition periods, and larval instar development were not different significantly among the food hosts. Number of eggs laid varied significantly among the cereal grains; mean fecundity was highest on maize (67.2±3.16 and lowest on millet (53.8±0.17. Number of immature (larva and pupa and adult stages varied significantly among the cereal grains. There exist four larval instars with a varied mean head capsule width, with a mean total instar larval developmental period of 23.1, 22.2, 22.2, and 21.6 d on maize, rice, sorghum, and millet, respectively. There was linear relationship and significant correlation between the stages of larval development and head capsule width. The mean developmental period from egg to adult varied, being highest on maize (34.7 d and lowest on sorghum (33.5 d.

  17. Erotylidae (Insecta, Coleoptera of Poland – problematic taxa, updated keys and new records

    Directory of Open Access Journals (Sweden)

    Rafal Ruta

    2011-10-01

    Full Text Available New data concerning the occurrence of pleasing fungus beetles (Coleoptera: Erotylidae in Poland are given, with a focus on rare and difficult to identify Central European taxa. Cryptophilus cf. integer (Heer (Cryptophilinae is reported from the Polish territory for the first time based on adult and larval specimens collected in the Wielkopolska-Kujawy Lowland. Identification problems concerning species of Cryptophilus introduced to Europe are discussed. Triplax carpathica Reitter (Erotylinae is recorded from the Białowieża Primeval Forest, which is the first known non-Carpathian finding of this species, located in the close proximity of the Polish-Belarussian UNESCO World Heritage Site “Białowieża Forest”. Discussion of T. carpathica being conspecific with Siberian T. rufiventris Gebler is provided. New Polish localities of several other Erotylidae are reported, and an updated key to Central European species of Triplax is given. The Triplax key is supplemented with dorsal and ventral habitus images of all treated Triplax species. One of the rarest Central European erotyline species Combocerus glaber (Schaller is recorded from xerothermic grasslands in North-West Poland.

  18. Fruit Damage Patterns Caused by Ovipositing Females of Conotrachelus dimidiatus (Coleoptera: Curculionidae in Guava Trees

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    Felipe Tafoya

    2010-01-01

    Full Text Available We evaluated the damage patterns produced by females of the guava weevil Conotrachelus dimidiatus Champion, 1904 (Coleoptera: Curculionidae, according to the position of the damaged fruit in guava trees Psidium guajava L. in Calvillo, Aguascalientes, Mexico. The trees were subdivided in eight zones, and during one year the level of fruit lesions due to oviposition was registered. Results showed a higher level of damage in the upper and external zone of the trees (P≤.05. We found no significant differences in damage between the four cardinal points (P≥.05. During the year, the level of damage was recorded and was higher in the months of August and September (P≤.05 associated with rainfall (0.86, P=.06 and increase in temperature (0.84, P=.03. The most susceptible fruits were in the size range of 2.1–4.0 cm (polar diameter. The information from this study will be used to design and establish effective control strategies for the guava weevil, taking into account location of the most susceptible fruits, seasonality of the pest, and the abiotic factors.

  19. Advances in the use of trapping systems for Rhynchophorus ferrugineus (Coleoptera: Curculionidae): traps and attractants.

    Science.gov (United States)

    Vacas, S; Primo, J; Navarro-Llopis, V

    2013-08-01

    Given the social importance related to the red palm weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae), efforts are being made to develop new control methods, such as the deployment of trapping systems. In this work, the efficacy of a new black pyramidal trap design (Picusan) has been verified in comparison with white and black buckets. In addition, the attractant and synergistic effect of ethyl acetate (EtAc) at different release levels has been evaluated under field conditions. The results show that Picusan traps captured 45% more weevils than bucket-type traps, offering significantly better trapping efficacy. The addition of water to traps baited with palm tissues was found to be essential, with catches increasing more than threefold compared with dry traps. EtAc alone does not offer attractant power under field conditions, and the release levels from 57 mg/d to 1 g/d have no synergistic effect with ferrugineol. Furthermore, significantly fewer females were captured when EtAc was released at 2 g/d. The implications of using EtAc dispensers in trapping systems are discussed.

  20. On the spatial spread of the Rice Water Weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Erirhinidae, in Italy

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    Daniela Lupi

    2010-07-01

    Full Text Available A five year study has been made to establish the spread of the rice water weevil Lissorhoptrus oryzophilus (Coleoptera: Erirhinidae in Northern Italy. Data obtained with GPS from 2005 throughout 2009 were first georeferenced with SW ArcGis® 9.2, then overlapped and compared to the map of the European environmental landscape based on the interpretation of satellite images (CORINE Land Cover map and to the hydrographic chart CT10 (Technical Regional map 10000. The analysis of the radial rate of spread per year indicates a deceleration in the expansion from 10.864 ± 6.801 km/year in 2005 to 5.318 ± 1.401 km/year in 2009. In five years the weevil has expanded its distribution in nearly all rice paddies in Lombardy and Piedmont, over an area of about 200,000 ha, which correspond to 86% of the total Italian rice area. Its expansion is thought to follow a type of stratified dispersal, due both to insect adult active dispersal and to accidental movements caused by human transportation.

  1. Transcriptomic Analysis of Eucryptorrhynchus chinensis (Coleoptera: Curculionidae) Using 454 Pyrosequencing Technology

    Science.gov (United States)

    Liu, Zhen-Kai; Wen, Jun-Bao

    2016-01-01

    Eucryptorrhynchus chinensis Olivier (Coleoptera: Curculionidae) is one of the most important pests of Ailanthus altissima; however, so far, no studies on the genome or transcriptome of E. chinensis have been reported. Using the Roche 454 FLX Titanium platform, an RNA pool obtained from E. chinensis eggs, larva, pupae, and adults was sequenced and assembled de novo to achieve maximum diversity of sampled transcripts. We obtained 1,441,137 (∼518 Mb) raw reads with an average length of 360 bp. After trimming, 89% qualified reads were produced and assembled into 35,509 isotigs with an average length of 440 bp, N50 of 1,048 bp, and 111,643 singletons. We generated 87,894 unigenes following a cluster analysis of the isotigs and singletons, and then functionally annotated the unigenes with gene descriptions. We obtained 23,363 GO assignments, and 12,724 unigenes were assigned to KOG. Based on these annotations, 294 biochemical pathways involved in growth, reproduction, and stress or immune responses were predicted. A total of 659,026 single nucleotide variants and 6,112 simple sequence repeats were detected. Our data provide comprehensive information on the sequence and possible functions of E. chinensis transcripts.

  2. Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil

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    Andreia Mauruto Chernaki-Leffer

    2011-03-01

    Full Text Available Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer, is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvos. Larvae were fed rabbit feed wetted with a triflumuron-water solution. Concentration-mortality regressions were estimated using Probit analysis and resistance ratios were calculated based on the susceptible population. Among the field populations evaluated, cypermethrin LC50 values for adults, ranged from 68.1 to 6,263 ng (AI/cm². LC50 values for adults challenged with dichlorvos ranged from 10.3 to 1,385 ng (AI/cm². One population from Pato Branco showed reduced susceptibility to triflumuron (LC50 = 272 µg (AI/ml of solution when compared to the most susceptible population (LC50 = 109.8 µg (AI/ml. Application of cypermethrin and dichlorvos analogues should be managed with caution to minimize insecticide resistance problems.

  3. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

    Science.gov (United States)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2015-06-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread.

  4. Species limits in polymorphic mimetic Eniclases net-winged beetles from New Guinean mountains (Coleoptera, Lycidae).

    Science.gov (United States)

    Bocek, Matej; Bocak, Ladislav

    2016-01-01

    Species delimitation was compared in a group of closely related lineages of aposematically colored Eniclases (Coleoptera, Lycidae) using morphology, genetic distances, and Bayesian implementation of the Poisson Tree Processes model. A high diversity of net-winged beetles was found in previously unsampled regions of New Guinea and ten new species are described: Eniclases bicolor sp. n., Eniclases bokondinensis sp. n., Eniclases brancuccii sp. n., Eniclases elelimensis sp. n., Eniclases infuscatus sp. n., Eniclases niger sp. n., Eniclases pseudoapertus sp. n., Eniclases pseudoluteolus sp. n., Eniclases tikapurensis sp. n., and Eniclases variabilis sp. n. Different levels of genetic and morphological diversification were identified in various sister-species pairs. As a result, both morphological and molecular analyses are used to delimit species. Sister-species with uncorrected pairwise genetic divergence as low as 0.45% were morphologically distinct not only in color pattern, but also in the relative size of eyes. Conversely, differences in color pattern regardless of their magnitude did not necessarily indicate genetic distance and intraspecific mimicry polymorphism was common. Additionally, genetic divergence without morphological differentiation was detected in one sister-species pair. Low dispersal propensity, diverse mimicry patterns, and mimetic polymorphism resulted in complex diversification of Eniclases and uncertain species delimitation in recently diversified lineages.

  5. Notas e novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae

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    Ubirajara R. Martins

    2007-01-01

    Full Text Available Novas espécies descritas do Brasil: Hypsioma carioca sp. nov. (Rio de Janeiro e Hesychotypa maraba sp. nov. (Pará; do Equador: Sternycha ecuatoriana sp. nov. (Pichincha; da Bolívia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. e Alexera secunda sp. nov. Transfere-se Hesycha strandi (Breuning, 1943 para o gênero Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 é considerada sinônima de H. miniata Thomson, 1868. Novo registro (Trinidad e figura são dados para Trachysomus surdus.Notes and new species of Onciderini (Coleoptera, Cerambycidae, Lamiinae. New species described from Brazil: Hypsioma carioca sp. nov. (Rio de Janeiro and Hesychotypa maraba sp. nov. (Pará; from Ecuador: Sternycha ecuatoriana sp. nov. (Pichincha; from Bolivia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. and Alexera secunda sp. nov. Hesycha strandi (Breuning, 1943 is transferred to the genus Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 is considered a synonym of H. miniata Thomson, 1868. New record (Trinidad and figure for Trachysomus surdus Dillon & Dillon, 1946 are given.

  6. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect

    International Nuclear Information System (INIS)

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5–7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1–4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. (author)

  7. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae

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    M.M. Kiradoo

    2011-03-01

    Full Text Available The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insecticides, there is an utmost need to search for some alternate methods for protection against the damage caused by insects. The leaves of the select plants viz., Ocimum basilicum, O. sanctum and Mentha spicata were employed in the form of various formulations and mortality of the pest insect was assessed. A significant increase in mortality of C. chinensis was observed in all the experimental sets as compared to normal and control sets during the present study. When comparisons were made on the basis of ANOVA to compare the effect of the three plants on mortality, it was found that the treatments of O. basilicum were superior over M. spicata and O. sanctum. The findings overall suggest that all the three plants screened have a potential to be used against the pest C. chinensis and can be employed as an alternate to chemical insecticides in household and storehouses to minimize the infestation and damage caused by the bruchid.

  8. Ecological, morphological, and histological studies on Blaps polycresta (Coleoptera: Tenebrionidae) as biomonitors of cadmium soil pollution.

    Science.gov (United States)

    Osman, Wafaa; El-Samad, Lamia M; Mokhamer, El-Hassan; El-Touhamy, Aya; Shonouda, Mourad

    2015-09-01

    Soil pollution in Egypt became far more serious than before due to either the heavy usage of different toxic pesticides or aerosol deposition of industrial pollutants. The present mentioned ground beetle, Blaps polycresta Tschinkel 1975 (Coleoptera: Tenebrionidae), showed ecological, morphological, and histological alterations in adult insects as biomonitors. Two cultivated sites (reference and polluted) were chosen for sampling the insects. The results indicated a significant increase in soil cadmium concentration of the polluted site leading to sex-specific difference in cadmium accumulation in gonads and alimentary canal of insects that being higher in males than females. The cadmium pollution leads significantly to a decrease in population density, a reduction in body weight, an increase in mortality rate, and an increase in sex ratio of the insects. The results also revealed a striking decrease in body length of the polluted insects with a marked increase in the percentage of deformed gonads and alimentary canal of both sexes. Some histopathological alterations were also recorded in testis, ovary, and midgut of the polluted insects. Our results confirmed that beetles are a good bioindicator for soil pollution, and the different studied parameters could be easily employed as sensitive monitors for cadmium soil pollution.

  9. Caracteres preimaginales y aspectos bionómicos de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae

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    Jerez, V.

    2011-12-01

    Full Text Available We describe the morphology and microstructure of the egg chorion, and the morphology of the first instar larva of Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini. Bionomical and distributional data on this species are also provided. To obtain eggs and larvae, couples were collected in the field and kept in rearing cages until oviposture and ecclosion. The structure and adornment of the egg exochorion, and the exterior morphological features of larvae were examined with electron scanning microscopy. The eggs of G. luczotii showed a rounded micropyle and a smooth exochorion, composed of hexagonal cells without aeropyles. The larvae of G. luczotii showed morphological characteristics suited for an edaphic life similar to that of Pedobionta: digging prothoracic legs, cephalic capsule with abundant sensilla, and well–developed pygopodium. We analysed the importance of larval morphology as an element for specific diagnosis and found that interspecific differences regarding frontal sensilla, clypeus shape, and anterior part of labrum, had a taxonomic value and possibly a phylogenetic value.

  10. BIOECOLOGY AND MANAGEMENT OF ERVA-MATE BORER, Hedypathes betulinus (KLUG, 1825 (COLEOPTERA: CERAMBYCIDAE

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    Márcia d´Avila

    2010-08-01

    Full Text Available The erva-mate (Ilex paraguariensis A.St.-Hil. has a social, cultural and economic importance in  the southern states of Brazil. The  pure stands of  this culture was responsible for the increase  of many species of insects. Hedypathes betulinus (Klug, 1825 (Coleoptera: Cerambycidae is considered the main pest from an economic viewpoint because of its difficult control and potential for damage. The larval phase occurs inside the twings and trunks, what makes more difficult to deal with its detention and management. Cultural and mechanical management are the most indicated, such as collection of adults, prunning and burning of plant parts damaged by the insect, balanced nutrition, adequate plant density and maintenance of areas with native vegetation or also the introduction of policulture. These strategies  may increase  the  agroecossystem  balance  and  thus  a  reduction  of  the  insect-pest  to  an aceptable level. Therefore, the objective of this study was to assemble and the discuss the information on the bioecology and management of erva-mate borer.

  11. Karyotype differentiation patterns in species of the subfamily Scarabaeinae (Scarabaeidae, Coleoptera).

    Science.gov (United States)

    Cabral-de-Mello, Diogo Cavalcanti; de Oliveira, Sárah Gomes; Ramos, Ituza Celeste; de Moura, Rita de Cássia

    2008-12-01

    The aim of this study was to describe the karyotype of species belonging to the subfamily Scarabaeinae (Coleoptera, Scarabaeidae) and to compile the conventional cytogenetic data available in the literature for this group. The karyotypes of ten species belonging to the tribes Canthonini, Coprini, Onthophagini and Phanaeini were analyzed by conventional staining. Eight of these species were described for the first time (Canthon aff carbonarius, Canthon chalybaeus, Coprophanaeus dardanus, Deltochilum aff amazonicum, Dichotomius geminatus, Oxysternon silenus, Phanaeus chalcomelas and Malagoniella aff astyanax) and two were redescribed (Diabroctis mimas and Digitonthophagus gazella) since their karyotypes differed from those previously published in the literature. Four species studied showed a diploid number of 2n=20 and a parachute type sex determining system and the karyotype was 2n=20,Xy in two species and 2n=18,Xy(p), 2n=19,X0, 2n=12,XY and 2n=14,neoXY in one each. The chromosome morphology of the different species varied, with the observation of metacentric, submetacentric, subacrocentric and acrocentric chromosomes. The X chromosome was predominantly meta or submetacentric in the species analyzed, whereas the y chromosome presented two arms or was punctiform. In conclusion, the subfamily Scarabaeinae comprises 120 species analyzed cytogenetically, and are observed the occurrence of five chromosome rearrangements (autosome-autosome and X-autosome fusions, pericentric inversions, fissions and loss of the y chromosome) that are related to the chromosome variability and evolution in the group. PMID:18495484

  12. A multilayer micromechanical model of the cuticle of Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae).

    Science.gov (United States)

    Andrew Jansen, M; Singh, Sudhanshu S; Chawla, Nikhilesh; Franz, Nico M

    2016-08-01

    Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae), is a weevil species common throughout the southwestern United States that uses its rostrum - a very slender, curved, beak-like projection of the head - to excavate tunnels in plant organs (such as acorns) for egg laying (oviposition). Once the apical portion of the rostrum has been inserted into the preferred substrate for oviposition, the female begins rotating around the perimeter of the hole, elevating her head by extending the fore-legs, and rotating the head in place in a drilling motion. This action causes significant elastic deformation of the rostrum, which will bend until it becomes completely straight. To better understand the mechanical behavior of the cuticle as it undergoes deformation during the preparation of oviposition sites, we develop a comprehensive micro/macro model of the micromechanical structure and properties of the cuticle, spanning across all cuticular regions, and reliably mirroring the resultant macroscale properties of the cuticle. Our modeling approach relies on the use of multi-scale, hierarchical biomaterial representation, and employs various micromechanical schemata - e.g., Mori-Tanaka, effective field, and Maxwell - to calculate the homogenized properties of representative volume elements at each level in the hierarchy. We describe the configuration and behavior of this model in detail, and discuss the theoretical implications and limitations of this approach with emphasis on future biomechanical and comparative evolutionary research. Our detailed account of this approach can thereby serve as a methodological template for exploring the biomechanical behavior of new insect structures. PMID:27189867

  13. Coexistence and Competition between Tomicus yunnanensis and T. minor (Coleoptera: Scolytinae in Yunnan Pine

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    Rong Chun Lu

    2012-01-01

    Full Text Available Competition and cooperation between bark beetles, Tomicus yunnanensis Kirkendall and Faccoli and Tomicus minor (Hartig (Coleoptera: Scolytinae were examined when they coexisted together in living Yunnan pine trees (Pinus yunnanensis Franchet in Yunnan province in Southwest China. T. yunnanensis bark beetles were observed to initiate dispersal from pine shoots to trunks in November, while the majority of T. minor begins to transfer in December. T. yunnanensis mainly attacks the top and middle parts of the trunk, whereas T. minor mainly resides in the lower and middle parts of the trunk. The patterns of attack densities of these two species were similar, but with T. yunnanensis colonizing the upper section of the trunk and T. minor the lower trunk. The highest attack density of T. Yunnanensis was 297 egg galleries/m2, and the highest attack density of T. minor was 305 egg galleries/m2. Although there was significant overlap for the same bark areas, the two species generally colonize different areas of the tree, which reduces the intensity of competition for the relatively thin layer of phloem-cambium tissues where the beetles feed and reside.

  14. Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera)

    Science.gov (United States)

    Pineda-Castellanos, Mónica L.; Rodríguez-Segura, Zitlhally; Villalobos, Francisco J.; Hernández, Luciano; Lina, Laura; Nuñez-Valdez, M. Eugenia

    2015-01-01

    Serratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarabaeidae) larvae collected from cornfields in Mexico. Five isolates were identified as Serratia marcescens by 16S rRNA gene sequencing and biochemical tests. Oral and injection bioassays using healthy Phyllophaga blanchardi larvae fed with the S. marcescens isolates showed different degrees of antifeeding effect and mortality. No insecticidal activity was observed for Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) by oral inoculation. S. marcescens (Sm81) cell-free culture supernatant caused significant antifeeding effect and mortality to P. blanchardi larvae by oral bioassay and also mortality by injection bioassay. Heat treated culture broths lost the ability to cause disease symptoms, suggesting the involvement of proteins in the toxic activity. A protein of 50.2 kDa was purified from the cell-free broth and showed insecticidal activity by injection bioassay towards P. blanchardi. Analysis of the insecticidal protein by tandem- mass spectrometry (LC-MS/MS) showed similarity to a Serralysin-like protein from S. marcescens spp. This insecticidal protein could have applications in agricultural biotechnology. PMID:25984910

  15. Ground beetles (Coleoptera, Carabidae of the Hanford Nuclear Site in south-central Washington State

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    Chris Looney

    2014-04-01

    Full Text Available In this paper we report on ground beetles (Coleoptera: Carabidae collected from the Hanford Nuclear Reservation and Hanford National Monument (together the Hanford Site, which is located in south-central Washington State. The Site is a relatively undisturbed relict of the shrub-steppe habitat present throughout much of the western Columbia Basin before the westward expansion of the United States. Species, localities, months of capture, and capture method are reported for field work conducted between 1994 and 2002. Most species were collected using pitfall traps, although other capture methods were employed. Trapping results indicate the Hanford Site supports a diverse ground beetle community, with over 90% of the 92 species captured native to North America. Four species collected during the study period are newly recorded for Washington State: Bembidion diligens Casey, Calosoma obsoletum Say, Pseudaptinus rufulus (LeConte, and Stenolophus lineola (Fabricius. Based on these data, the Site maintains a diverse ground beetle fauna and, due to its size and diversity of habitats, is an important repository of shrub-steppe biodiversity.

  16. Ground beetles (Coleoptera, Carabidae) of the Hanford Nuclear Site in south-central Washington State

    Science.gov (United States)

    Looney, Chris; Zack, Richard S.; LaBonte, James R.

    2014-01-01

    Abstract In this paper we report on ground beetles (Coleoptera: Carabidae) collected from the Hanford Nuclear Reservation and Hanford National Monument (together the Hanford Site), which is located in south-central Washington State. The Site is a relatively undisturbed relict of the shrub-steppe habitat present throughout much of the western Columbia Basin before the westward expansion of the United States. Species, localities, months of capture, and capture method are reported for field work conducted between 1994 and 2002. Most species were collected using pitfall traps, although other capture methods were employed. Trapping results indicate the Hanford Site supports a diverse ground beetle community, with over 90% of the 92 species captured native to North America. Four species collected during the study period are newly recorded for Washington State: Bembidion diligens Casey, Calosoma obsoletum Say, Pseudaptinus rufulus (LeConte), and Stenolophus lineola (Fabricius). Based on these data, the Site maintains a diverse ground beetle fauna and, due to its size and diversity of habitats, is an important repository of shrub-steppe biodiversity. PMID:24715791

  17. FAUNÍSTIC STUDY OF BEETLES (COLEOPTERA IN A SILVICULTURAL-PASTORAL SYSTEM

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    Alexander Machado Auad

    2011-03-01

    Full Text Available The aim of this study was to conduct a survey of beetles (Coleoptera in a silvicultural-pastoral system, estimating constancy, abundance, richness, diversity of families and seasonality, from July 2006 to June 2008. The study was carried out at the Embrapa Dairy Cattle station, in Coronel Pacheco, Minas Gerais, Brazil. The beetles were collected in Malaise traps, every two weeks, and taken to the laboratory for analysis. A total of 26 families, 294 morphospecies and 1,606 specimens were found. The Elateridae, Mordelidae, Chrysomelidae, Coccinellidae and Curculionidae families were the most abundant, rich and diverse. The Scarabaeidae and Scolytidae families were also among the most abundant, and the Cerambycidae family was among the richest, while the Bruchidae and Carabidae families presented high diversity.  The largest number of individuals and morphospecies sampled occurred during the period of rain. November 2007 presented the most (n = 535 specimens captures of any month, followed by October 2006, September 2007, March and April of 2008.

  18. South American Coccinellidae (Coleoptera: part X: A systematic revision of Thalassa Mulsant (Hyperaspidinae

    Directory of Open Access Journals (Sweden)

    Julianne Milléo

    2004-01-01

    Full Text Available The genus Thalassa Mulsant, 1850 is revised. Its five species are redescribed: T. pentaspilota (Chevrolat, 1853, T. flaviceps Mulsant, 1850, T. similaris Mulsant, 1850, T. montezumae Mulsant, 1850, and T. glauca (Mulsant, 1850. A new species is proposed, Thalassa korschefskyi sp.nov., from Colombia. The species are illustrated and a key to species is also provided. Lectotype of Thalassa reyi Mulsant, 1850 is here designated.Coccinellidae (Coleoptera da América do Sul. Parte X: Revisão sistemática de Thalassa Mulsant (Hyperaspidinae. O gênero Thalassa Mulsant, 1850 é revisado. Suas cinco espécies são redescritas: T. pentaspilota (Chevrolat, 1853, T. flaviceps Mulsant, 1850, T. similaris Mulsant, 1850, T. montezumae Mulsant, 1850 e T. glauca (Mulsant, 1850. Uma nova espécie é proposta, Thalassa korschefskyi sp. nov., da Colômbia. São fornecidas ilustrações e chave para as espécies. O lectótipo de Thalassa reyi Mulsant, 1850 é aqui designado.

  19. Naturally-Occurring Entomopathogenic Fungi on Three Bark Beetle Species (Coleoptera: Curculionidae in Bulgaria

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    Slavimira A. Draganova

    2010-01-01

    Full Text Available Bark beetles (Coleoptera: Curculionidae: Scolytinae belong to one of the most damaging groups of forest insects and the activity of their natural enemies –pathogens, parasitoids,parasites or predators suppressing their population density,is of great importance. Biodiversity of entomopathogenic fungi on bark beetles in Bulgaria has been investigated sporadically. The aim of this preliminary study was to find, identify and study morphological characteristics of fungal entomopathogens naturally-occurring in populations of three curculionid species – Ips sexdentatus Boern, Ips typographus (L. and Dryocoetes autographus (Ratz.. Dead pest adults were found under the bark of Pinus sylvestris and Picea abies trees collectedfrom forests in the Maleshevska and Vitosha Mountains. Fungal pathogens were isolated into pure cultures on SDAY (Sabouraud dextrose agar with yeast extract and were identified based on morphological characteristics both on the host and in a culture.Morphological characteristics of the isolates were studied by phenotypic methods. The fungal isolates obtained from dead adults of Ips sexdentatus, Ips typographus and D. autographus were found to belong to the species Beauveria bassiana (Bals. – Criv. Vuillemin,Beauveria brongniartii (Saccardo Petch and Isaria farinosa (Holmsk. Fries (anamorph Ascomycota, Sordariomycetes: Hypocreales, Cordycipitaceae. Morphological traits of the isolates are described.

  20. Pitfall Traps and Mini-Winkler Extractor as Complementary Methods to Sample Soil Coleoptera.

    Science.gov (United States)

    Carneiro, A C; Batistella, D A; Battirola, L D; Marques, M I

    2016-02-01

    We compared abundance, species richness, and capture efficiency with pitfall traps and mini-Winkler extractors to examine their use as complementary methods for sampling soil Coleoptera during dry (2010) and high water seasons (2011) in three areas, including inundated and non-inundated regions, in the Pantanal of Poconé, Mato Grosso, Brazil. We paired treatments with two 10 × 10 m plots in inundated and non-inundated locations that were repeated three times in each location for a total of 18 plots. In each plot, we used nine pitfall traps and collected 2 m(2) of leaf litter and surface soil samples with mini-Winkler extractors. We collected a total of 4260 adult beetles comprising 36 families, 113 genera, and 505 species. Most were caught in pitfalls (69%) and the remainder in the mini-Winkler extractors (31%). Each method provided distinct information about the beetle community: 252 species were captured only in pitfall traps, 147 using only the mini-Winkler extractors, and these methods shared another 106 species. Pitfall and mini-Winkler contribute in different ways for the sampling of the soil beetle community, and so they should be considered complementary for a more thorough assessment of community diversity. PMID:26493175

  1. Oil pipeline corridor through an intact forest alters ground beetle (Coleoptera: Carabidae) assemblages in southeastern Ohio.

    Science.gov (United States)

    Silverman, Bareena; Horn, David J; Purrington, Foster F; Gandhi, Kamal J K

    2008-06-01

    Litter-dwelling ground beetle (Coleoptera: Carabidae) assemblages were monitored 1 yr after the construction of a corridor for installation of an oil pipeline along a xeric ridge-top forest in southeastern Ohio. After the creation of the corridor, three distinct habitats were evident in these sites: open corridor, ecotone areas around the corridor, and undisturbed forest interior. Carabidae were collected using directional pitfall traps that were placed parallel and perpendicular to the corridor in each of the three habitats. Results indicate that more carabids were present in the ecotone than in the other two habitats. Carabid diversity as estimated by rarefaction was highest in the corridor followed by ecotone and forest interior. Generalist and forest specialists such as Synuchus impunctatus (Say), Carabus goryi Dejean, and Pterostichus trinarius (Casey) were present in greater numbers in the forest interior and ecotone assemblages. In contrast, open-habitat specialists such as Harpalus pensylvanicus (DeGeer) and Selenophorus opalinus (LeConte) were present in greater numbers in the corridor assemblages. Carabid assemblages of the corridor were distinct from those of the ecotone and forest interior, whereas the latter two habitats had very similar assemblages. The successional pathway of the corridor carabid assemblage will therefore be likely different from that of the forest interior and ecotone. Overall, results indicate that construction of the oil pipeline corridor had significant short-term effects on the carabid numbers, diversity, and species composition because of ensuing habitat changes and fragmentation of the forest. PMID:18559178

  2. Demographic comparison and population projection of Rhynchophorus ferrugineus (Coleoptera: Curculionidae) reared on sugarcane at different temperatures.

    Science.gov (United States)

    Peng, Lu; Miao, Yunxin; Hou, Youming

    2016-01-01

    Understanding how temperature affects fitness is important for conservation and pest management, especially in the era of global climate change. Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae) is a worldwide pest of many economically important crops. Although much is known about this pest's life cycle, its adaptability to different temperatures is not fully understood. Here, we used age- and stage-specific life tables to investigate the effects of temperature on fitness-related traits and demographic parameters of R. ferrugineus under eight constant temperature regimens in the laboratory. The growth potential of these populations was also evaluated. The greatest longevity for males and females was 158.0 d at 24 °C and 144.5 d at 21 °C, respectively, but mean total fecundity was the highest at 27 °C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0) increased initially at low temperatures and then decreased. All metrics reached a maximum at 27 °C and a minimum at 36 °C. Mean generation times (T ) decreased across the temperature range with a minimum at 36 °C. Our results indicate that the optimum temperature for growth of R. ferrugineus was approximately 27 °C. Our work will be of value for developing strategies for control management of this pest species. PMID:27545594

  3. Volatiles released from Vaccinium corymbosum were attractive to Aegorhinus superciliosus (Coleoptera: Curculionidae) in an olfactometric bioassay.

    Science.gov (United States)

    Parra, Leonardo; Mutis, Ana; Ceballos, Ricardo; Lizama, Marcelo; Pardo, Fernando; Perich, Fernando; Quiroz, Andrés

    2009-06-01

    The objective of this study was to evaluate the role of host volatiles in the relationship between a blueberry plant Vaccinium corymbosum L. and the raspberry weevil Aegorhinus superciliosus (Guérin) (Coleoptera: Curculionidae), the principal pest of blueberry in the south of Chile. Volatiles from the aerial part of different phenological stages of the host were collected on Porapak Q and analyzed by coupled gas chromatography-mass spectrometry (GC-MS). Several chemical groups were identified including green leaf volatiles, aromatic compounds, and terpenes. The olfactometric responses of A. superciliosus toward different odor sources were studied in a four-arm olfactometer. Blueberry shoots at the phenological stages of fruit set, and blue-pink fruit color elicited the greatest behavioral responses from weevils. Five compounds (2-nonanone, eucalyptol, R- and S-limonene, and 4-ethyl benzaldehyde) elicited an attractant behavioral response from A. superciliosus. The results suggest the host location behavior of A. superciliosus could be mediated by volatiles derived from V. corymbosum. This work has identified a number of compounds with which it is possible to develop a lure for the principal pest of blueberry in southern Chile.

  4. Males of Hylamorpha elegans burmeister (Coleoptera: Scarabaeidae) are attracted to odors released from conspecific females.

    Science.gov (United States)

    Quiroz, Andrés; Palma, Ruben; Etcheverría, Paulina; Navarro, Vicente; Rebolledo, Ramón

    2007-04-01

    The behavioral responses of Hylamorpha elegans L. (Coleoptera: Scarabaeidae, Rutelinae) to the semiochemicals released from conspecific individual adults were studied, with particular attention paid to female attraction of males. Odors released from virgin females significantly attracted male conspecifics in both the field and laboratory olfactometer and wind tunnel bioassays. However, females did not attract other females, and males attracted no one. The response of male H. elegans to (1) compounds (1,4-hydroquinone and 1,4-benzoquinone) released only by unmated females; (2) the essential oil of the secondary host (Nothofagus obliqua); and (3) the blend of 1,4-hydroquinone and 1,4-benzoquinone with N. obliqua essential oil was studied. The blend of 1,4-benzoquinone mixed with essential oil at the trial concentration was attractive with males. The same response was found with 1,4-hydroquinone alone. The essential oil did not have the expected attractant effect on conspecific males. These results suggest that, when combined with essential oil, 1,4-benzoquinone may function in the sexual behavior of males and females. These findings are discussed in terms of the ecological role of this putative sexual pheromone and its potential use in a strategy of control of this pest.

  5. The Hydraulic Mechanism of the Unfolding of Hind Wings in Dorcus titanus platymelus (Order: Coleoptera

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    Jiyu Sun

    2014-04-01

    Full Text Available In most beetles, the hind wings are thin and fragile; when at rest, they are held over the back of the beetle. When the hind wing unfolds, it provides the necessary aerodynamic forces for flight. In this paper, we investigate the hydraulic mechanism of the unfolding process of the hind wings in Dorcus titanus platymelus (Oder: Coleoptera. The wing unfolding process of Dorcus titanus platymelus was examined using high speed camera sequences (400 frames/s, and the hydraulic pressure in the veins was measured with a biological pressure sensor and dynamic signal acquisition and analysis (DSA during the expansion process. We found that the total time for the release of hydraulic pressure during wing folding is longer than the time required for unfolding. The pressure is proportional to the length of the wings and the body mass of the beetle. A retinal camera was used to investigate the fluid direction. We found that the peak pressures correspond to two main cross-folding joint expansions in the hind wing. These observations strongly suggest that blood pressure facilitates the extension of hind wings during unfolding.

  6. Registros y distribución de la especie afroasiática Digitonthophagus gazella (Coleoptera: Scarabaeidae: Scarabaeinae en Argentina Records and distribution of the Afro Asian species Digitonthophagus gazella (Coleoptera: Scarabaeidae: Scarabaeinae in Argentina

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    María C. Álvarez Bohle

    2009-12-01

    Full Text Available Se cita por primera vez el escarabajo coprófago Digitonthophagus gazella Fabricius (Coleoptera: Scarabaeidae para Argentina, por lo que se amplía su área de distribución geográfica en Sudamérica. Esta especie de origen afro-asiático fue capturada mediante colecta manual en estiércol de ganado bovino y equino, con trampas de luz y trampas de caída cebadas con estiércol en seis provincias del centro y norte argentino entre los años 2006 y 2009.The occurrence of the dung beetle Digitonthophagus gazella Fabricius (Coleoptera: Scarabaeidae is reported for the first time for Argentina. The record of this species represents an extension of its geographical range in South America. This species of Afro Asian origin was captured by manual collection in cattle dung, light traps, and dung baited pitfall traps in six provinces of Northern and Central Argentina between 2006 and 2009.

  7. Danos de Conotrachelus dubiae (Coleoptera: curculionidae em frutos de camu-camu (Myrciaria dubia na Amazônia Central Damage of camu-camu (Myrciaria dubia fruits by Conotrachelus dubiae (Coleoptera: curculionidae in Central Amazonia

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    Sidney Alberto do Nascimento Ferreira

    2003-12-01

    Full Text Available No Brasil, a ocorrência de Conotrachelus dubiae O'Brien, 1995 (Coleoptera: Curculionidae em camu-camu [Myrciaria dubia (H.B.K. McVaugh, Myrtaceae] tinha sido constatada somente em populações naturais. Relata-se sua ocorrência em um cultivo experimental, onde se avaliou os danos de C. dubiae em frutos de camu-camu, em diferentes graus de amadurecimento, entre 1999 e 2003. Os danos causados pela larva aumentaram com o amadurecimento dos frutos, havendo maior comprometimento da polpa do fruto (30 a 90% do que das sementes (7%. A incidência desse inseto pode implicar em perdas quantitativas significativas na produção de camu-camu.In Brazil, the occurrence of Conotrachelus dubiae O'Brien, 1995 (Coleoptera: Curculionidae in camu-camu [Myrciaria dubia (H.B.K. McVaugh, Myrtaceae] had only been verified in natural populations. This report describes its occurrence in an experimental cultivation, where damage of camu-camu fruits by C. dubiae at different ripening stages was evaluated between 1999 and 2003. The damage caused by the larva increased with the degree of ripening of the fruits, with greater damage of fruit pulp (30 to 90% than to seeds (7%. The incidence of this insect may cause significant quantitative losses in the camu-camu production.

  8. Los coleópteros y heterópteros acuáticos del Parque Nacional Calilegua (Provincia de Jujuy, Argentina Aquatic Coleoptera and Heteroptera from Calilegua National Park (Jujuy Province, Argentina

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    Patricia L. M. Torres

    2008-06-01

    Full Text Available Se expone una lista de las especies de Coleoptera y Heteroptera presentes en los cuerpos de agua del Parque Nacional Calilegua. Se registraron 51 especies de Coleoptera incluidas en 28 géneros y cinco familias, y 37 especies de Heteroptera distribuidas en 22 géneros y 10 familias; sólo cuatro especies de Coleoptera y cinco de Heteroptera fueron citadas previamente del parque. Se mencionan por primera vez para la Argentina dos especies de Veliidae (Heteroptera: Rhagovelia trista Gould y Platyvelia brachialis (Stål, así como una especie y un género de Dytiscidae (Coleoptera: Desmopachria chei K. B. Miller y Bidessonotus Régimbart. Además, es la primera ocasión en que se mencionan para la provincia de Jujuy 24 especies y 11 géneros de Coleoptera (Leuronectes Sharp, Bidessonotus, Hypodessus Guignot, Desmopachria Babington, Celina Aubé, Gyrinus Linné, Haliplus Latreille, Hydrocanthus Say, Suphis Aubé, Anacaena Thomson, Derallus Sharp y 19 especies y 12 géneros de Heteroptera (Mesovelia Mulsant & Rey, Hebrus Curtis, Merragata White, Microvelia Westwood, Rhagovelia Mayr, Platyvelia J. T. Polhemus & D. A. Polhemus, Limnogonus Stål, Curicta Stål, Ranatra Fabricius, Centrocorisa Lundblad, Pelocoris Stål, Neoplea Esaki & China.A list of the species of aquatic Coleoptera and Heteroptera occurring in Calilegua National Park is presented. Fifty-one species of Coleoptera included in 28 genera and five families, and 37 species of Heteroptera belonging to 22 genera and 10 families were recorded. Only four species of Coleoptera and five of Heteroptera had been previously cited from the park. Two species of Veliidae (Heteroptera (Rhagovelia trista Gould, Platyvelia brachialis (Stål, along with one species and one genus of Dytiscidae (Coleoptera (Desmopachria chei K. B. Miller, Bidessonotus Régimbart are new records for Argentina. Twenty-four species and 11 genera of Coleoptera (Leuronectes Sharp, Bidessonotus, Hypodessus Guignot, Desmopachria

  9. TOXICITY ASSAY OF BASSA AND MALATHION TO SITOPHILUS ORYZAE AND TRIBOLIUM CASTANEUM%巴沙和马拉硫磷对两种储粮害虫成虫的毒力测定

    Institute of Scientific and Technical Information of China (English)

    罗来凌; 刘伟强; 田世尧; 杨森辉; 赖志勇

    2002-01-01

    用97%巴沙和45%马拉硫磷乳油对米象(Sitophilus oryzae)和赤拟谷盗(Triboli-um castaneum)作毒力测定.根据结果求出巴沙和马拉硫磷处理米象成虫72小时毒力回归方程:巴沙为y=2.3960x-0.1760(r=0.9970),马拉硫磷为y=2.5642x-1.0915(r=0.9858);巴沙LG5o为144.6299mg/L(95%置信区间127.7332~161.5266mg/L),马拉硫磷LC50为237.4535mg/L(95%置信区间206.2068~268.7003mg/L).结果表明巴沙对供试米象的毒力比马拉硫磷大得多,但巴沙和马拉硫磷对供试赤拟谷盗的毒力均很小.

  10. Cowpea Vicilins: Fractionation of Urea Denatured Sub-Units and Effects on Callosobruchus maculatus F. (Coleoptera: Bruchidae Development

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    Antônio Chagas Mota

    2002-03-01

    Full Text Available Vicilins (7S storage globulins isolated from cowpea (Vigna unguiculata L. seeds which were susceptible (S and resistant (R to the cowpea weevil (Callosobruchus maculatus F., Coleoptera: Bruchidae were denatured by urea and fractionated by ion-exchange chromatography. Isolated fractions were incorporated in artificial seeds for assessment of their toxicity to C. maculatus. The most acidic fractions of both susceptible (CE-31 cultivar and resistant (IT81D-1045 line seeds were shown to affect development and survival of the bruchid. Results indicated that vicilin polypeptides of toxic nature were expressed in both types of storage globulins although at different levels.Vicilinas (globulinas de reserva 7S isoladas de sementes de feijão-de-corda (Vigna unguiculata L., susceptíveis (S e resistentes (R ao caruncho/gorgulho (Callosobruchus maculatus F., Coleoptera: Bruchidae foram desnaturadas por uréia e fracionadas por cromatografia de troca iônica. As frações isoladas foram incorporadas em sementes artificiais para avaliação de sua toxicidade a C. maculatus. As fracões mais ácidas de ambas vicilinas afetaram o desenvolvimento e a sobrevivência do bruquídeo. Sugerimos que polipeptídeos de vicilinas de natureza tóxica são expressos em ambos tipos de globulinas de reserva, embora em níveis diferentes.

  11. Longhorn beetles of the Ficuzza woods (W Sicily, Italy and their relationship with plant diversity (Coleoptera, Cerambycidae

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    Tommaso La Mantia

    2010-12-01

    Full Text Available The woods in Sicily are the result of centuries of anthropogenic activities that have reduced the surface of woodand changed the original composition even with the introduction of alien species to native flora. The value interms of biodiversity of these forests remains, however, high for they are the last refuge areas for many animalsand plant species. This study was conducted within the Ficuzza woods (West Sicily, extended about 5,000hectares on the slopes of limestone-dolomite rock of Busambra (1615 m asl, within which lies the largestremaining forest area in western Sicily. It is an area with a wide diversity of vegetation, represented mainly bynative forests (holm oak, cork oak, deciduous oaks, groups of riparian vegetation, shrubs, bushes, grasslands,and of non-native forest formations (Pinus and Eucalyptus woods. The study on Cerambycidae in this area isfragmented and does not specify a relation the species with the surrounding vegetation. This study wasperformed by choosing among various groups of insects, xylophagous Coleoptera Cerambycidae; existingliterature data and extensive collected field data were reviewed. The analysis was also performed by thecollection of dead wood in order to distinguish the relationship between the plant species and coleoptera. Theresults summarize and supplement the data registered so far, shedding further light on the ecological role ofthis group of insects that are also valid biomarkers of the integrity and complexity of the forest.

  12. Morphological and genetic variation in Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae, Cicindelinae: implications for conservation

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    Serrano, A.

    2003-12-01

    Full Text Available The establishment of taxonomic and geographical boundaries is a common problem when analysing clinal distributions. This is of particular concern when the assessment of intraspecific groupings is required for conservation management. The tiger beetle Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae is a typical case in which two recognised subspecies are distributed in a clinal latitudinal fashion in the dune systems along the Atlantic coast of Portugal. This habitat is increasingly under threat, and conservation measures are needed. We investigated the validity of the two named subspecies, based on a re-analysis of elytral and genitalic measurements using multivariate analysis. We also analysed variation in mitochondrial Cytochrome Oxidase I gene for a total of six populations along the cline. Multivariate analysis supported the idea of a morphological cline and revealed a clear distinction of the southernmost population and also some degree of distinctiveness of the most northern populations, partially supporting the recognised subspecific ranking. The mtDNA analysis identified two main groups corresponding to northern and southern populations. Both sets of markers showed that variation within the C. lusitanica assemblage is complex, with the boundaries between morphological and mtDNA groups not in agreement. However, populations at either end of the distributional range are clearly distinct from each other, and should be considered as provisional units for conservation programmes.El reconocimiento de límites taxonómicos y geográficos de la variabilidad observada es un problema habitual cuando se analizan distribuciones clinales. Esto es particularmente problemático cuando se requiere la determinación de agrupamientos intraespecíficos para tomar medidas de conservación. El cicindélido Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae constituye un caso típico en el que dos subespecies reconocidas se hayan distribuidas a

  13. Riqueza del complejo chisa (Coleoptera: Melolonthidae en cuatro agroecosistemas del Cauca, Colombia

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    Morón R. Miguel A.

    2005-12-01

    Full Text Available La composición y variación de la riqueza del complejo de larvas Melolonthidae se examinó en cuatro circunstancias agroecológicas en los municipios de Caldono y Buenos Aires, Cauca (1400 a 1500 m.s.n.m, 21,5ºC. y 2000 mm/año de precipitación promedio. Quincenalmente, durante un año, se visitaron parcelas de media hectárea de yuca, pastizal, cafetal y bosque, en cada parcela y ocasión se realizaron 10 muestreos ( 1m2 por 15 cm de profundidad. Se colectaron 10.261 larvas pertenecientes a 32 especies y 12 géneros de Melolonthidae, así: Phyllophaga, Plectris, Astaena, Macrodactylus, Ceraspis, Barybas, Isonychus, Anomala Callistethus, Strigoderma, Leucothyreus, Cyclocephala. La riqueza de especies presentó variaciones estadísticamente significativas entre localidades (F=87.24 p =2.72-18, muestreos (F=22.29 p =5.18-13, parcelas (F= 23.39 p=1.40-13 así como entre la mayoría de interacciones. Los promedios de riqueza fueron: yuca (4.5 , pastizal (3.6, café (2.1 y bosque (2.2, cada uno con grandes fluctuaciones que hicieron la diferencia. Se discuten las curvas de isodensidades de cada parcela y sus implicaciones en el programa de manejo. Abstract Richness of the white-grub complex (Coleoptera: Melolonthidae in agroecosystems of the Cauca province, Colombia. The composition and variation in the richness of the complex of rhizophagous Melolonthidae larvae was examinated in four agroecological zones in the Municipality of Caldono (1400-1500 m, 21.5ºC, average rainfall 2000 mm/yr with complementary surveys in the neighbouring site of Buenos Aires, Cauca. Half-hectare plots (cassava, pastures, coffee and wooded areas were selected and visited every 15 days (total of 15 visits per plot, and a total of 10 samples were taken (1 m2 by 15 cm deep. In total, 10,261 larvae of 32 species were collected: Phyllophaga, Plectris, Astaena, Macrodactylus, Ceraspis, Barybas, Isonychus, Anomala Callistethus, Strigoderma, Leucothyreus, Cyclocephala

  14. Typhlocharis vicariantes del Estrecho de Gibraltar. I: Typhlocharis armata Coiffait, 1969 (Coleoptera, Caraboidea, Trechidae

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    Pérez González, S.

    2010-12-01

    Full Text Available This first part about the vicariant Typhlocharis from Gibraltar Strait is dedicated to Typhlocharis armata Coiffait, 1969; a second paper will cover T. silvanoides Dieck, 1869. The studied material was obtained in four field campaigns carried out in the south of Spain and north of Morocco between 1997 and 2001 (Zaballos & Banda, 2000; Zaballos, 2005. In these campaigns abundant specimens of Typhlocharis (Coleoptera, Caraboidea, Trechidae were collected in different localities, the majority of them belonging to the species T. armata Coiffait, 1969. Their study and the comparison with the type series of this species allowed its complete redescription and clarification of its status within the genus. Contrary to what has been considered traditionally, the marginal umbilicate series of elytra in T. armata is composed of three, instead of four posterior setae. The taxonomical affinities of this species are established, the morphological weight of the umbilicate group of elytral setae as a lineage and species group defining character is discussed, and the theory of Jeanne (1973 about the relation between shortening of elytra and umbilicate series reduction with a decrease in body size is revised. The dispersal abilities are evaluated and a biogeographical explanation for the new distribution of the species is proposed.Esta primera parte sobre las especies de Typhlocharis vicariantes del Estrecho de Gibraltar está dedicada a Typhlocharis armata Coiffait, 1969; mientras que en un segundo artículo se tratará T. silvanoides Dieck, 1869. El material estudiado procede de cuatro campañas entomológicas realizadas en el sur de España y en el norte de Marruecos entre 1997 y 2001 (Zaballos & Banda, 2000; Zaballos, 2005. En estas campañas se recolectaron, en localidades diferentes, abundantes ejemplares de Typhlocharis (Coleoptera, Caraboidea, Trechidae, la mayoría de los cuales han resultado pertenecer a la especie T. armata Coiffait, 1969. Su estudio y

  15. Contributions towards an understanding of the Atomariinae (Coleoptera, Cryptophagidae of Atlantic Canada

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    Christopher Majka

    2010-02-01

    Full Text Available The Atomariinae (Coleoptera: Cryptophagidae fauna of Atlantic Canada are surveyed. Ten species in the genus Atomaria are reported in the region, six of which, including Atomaria distincta Casey, Atomaria fuscata Schonherr, Atomaria lederi Johnson, Atomaria lewisi Reitter, Atomaria testacea Stephens, and Atomaria nigrirostris Stephens, are newly recorded in Canada. Atomaria lederi and A. lewisi are newly recorded in North America. Twenty-three new provincial records are reported. Atomaria curtula Casey, 1900  (syn. n. is designated as a synonym of Atomaria lewisi Reitter; Atomaria ovalis Casey, 1900 (syn. n. is designated as a synonym of Atomaria apicalis Erichson, 1846; and Atomaria saginata Casey, 1900 (syn. n. is designated as a synonym of Atomaria fuscata Schonherr. A key to species is provided, as are species descriptions. Records of all species are given and their distribution in the region is plotted. The global distribution of all Holarctic and adventive species is briefly summarized, and the jurisdictions in North America where they have been reported are compiled. Habitat and bionomic information is also provided for all species, both from within the region, and from observations in European studies. The fauna is briefly discussed from a zoogeographic perspective; two species are Nearctic in origin, five may be Holarctic in distribution, and three others may be adventive Palaearctic species, although further evidence is required to confirm such preliminary assessments. Similarly, because of the paucity of information on this genus, further fieldwork is required to fully discern the distribution of these spe- cies in Atlantic Canada. Three are apparently saproxylic species characteristic of forested habitats; three are primarily grassland species; and the remaining four are eurytopic species found in a wide variety of forested and open habitats.

  16. Flight behaviour and dispersal of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) adults using mark-release-recapture method.

    Science.gov (United States)

    Ávalos, J A; Balasch, S; Soto, A

    2016-10-01

    The flight ability and patterns of an insect influence its spread, and the study of its behaviour can be used to improve the strategies to control the pest. Regarding Rhynchophorus ferrugineus (Olivier) (Coleoptera: Dryophthoridae), one of the worst threats to palm trees worldwide, laboratory experiments have been conducted to analyze their flight potential. However, these data must be complemented with tests that allow us to know its flight behaviour and dispersal patterns under field conditions. Two mark-release-recapture experiments were conducted in areas with R. ferrugineus infestations. In the first, the effects of weevil sex, temperature, solar radiation, and relative humidity, on the take-off and flight mobility of adults were analyzed. The second experiment aimed to determine the maximum flight distance covered by adults in field. The take-off rate for R. ferrugineus males was significantly greater than for females, and was positively influenced by temperature (optimum take-off around 25°C) and solar radiation, both factors being highly correlated. Female weevil recaptures were significantly higher, especially as temperatures increased (optimum recapture around 21°C). Dispersal distances of weevil adults increased when temperatures rose, and while this insect tended to fly short distances (<500 m), it was able to cover up to 7 km. The dispersal of R. ferrugineus adults mainly occurred during the first 7 days after their release, and when relative humidity increased, their dispersal time was reduced. The results obtained will permit a more effective implementation of certain measures used to control R. ferrugineus, such as olfactory trapping or intensive surveillance around pest outbreaks.

  17. Organization and comparative analysis of the mitochondrial genomes of bioluminescent Elateroidea (Coleoptera: Polyphaga).

    Science.gov (United States)

    Amaral, Danilo T; Mitani, Yasuo; Ohmiya, Yoshihiro; Viviani, Vadim R

    2016-07-25

    Mitochondrial genome organization in the Elateroidea superfamily (Coleoptera), which include the main families of bioluminescent beetles, has been poorly studied and lacking information about Phengodidae family. We sequenced the mitochondrial genomes of Neotropical Lampyridae (Bicellonycha lividipennis), Phengodidae (Brasilocerus sp.2 and Phrixothrix hirtus) and Elateridae (Pyrearinus termitilluminans, Hapsodrilus ignifer and Teslasena femoralis). All species had a typical insect mitochondrial genome except for the following: in the elaterid T. femoralis genome there is a non-coding region between NADH2 and tRNA-Trp; in the phengodids Brasilocerus sp.2 and P. hirtus genomes we did not find the tRNA-Ile and tRNA-Gln. The P. hirtus genome showed a ~1.6kb non-coding region, the rearrangement of tRNA-Tyr, a new tRNA-Leu copy, and several regions with higher AT contents. Phylogenetics analysis using Bayesian and ML models indicated that the Phengodidae+Rhagophthalmidae are closely related to Lampyridae family, and included Drilus flavescens (Drilidae) as an internal clade within Elateridae. This is the first report that compares the mitochondrial genomes organization of the three main families of bioluminescent Elateroidea, including the first Neotropical Lampyridae and Phengodidae. The losses of tRNAs, and translocation and duplication events found in Phengodidae mt genomes, mainly in P. hirtus, may indicate different evolutionary rates in these mitochondrial genomes. The mitophylogenomics analysis indicates the monophyly of the three bioluminescent families and a closer relationship between Lampyridae and Phengodidae/Rhagophthalmidae, in contrast with previous molecular analysis. PMID:27060405

  18. Side-effects of glyphosate on the life parameters of Eriopis connexa (Coleoptera: Coccinelidae) in Argentina.

    Science.gov (United States)

    Mirande, L; Haramboure, M; Smagghe, G; Piñeda, S; Schneider, M I

    2010-01-01

    In Argentina, transgenic soybean crop (Roundup Ready, RR) has undergone a major expansion over the last 15 years, with the consequent increase of glyphosate applications, a broad-spectrum and post emergence herbicide. Soybean crops are inhabited by several arthropods. Eriopis connexa Germar (Coleoptera: Coccinelidae) is a predator associated to soybean soft-bodies pest and have a Neotropical distribution. Nowadays, it is being considered a potentially biological control agent in South America. The objectives of this work were to evaluate the side-effects of glyphosate on larvae (third instar) and adults of this predator. Commercial compound and the maximum registered concentrations for field use were employed: GlifoGlex 48 (48% glyphosate, 192 mg a.i./litre, Gleba Argentina S.A.). The exposure was by ingestion through the treated prey (Rophalosiphum padi) or by drinking treated water during 48 h for treatment of the adult. The herbicide solutions were prepared using distilled water as solvent. The bioassays were carried out in the laboratory under controlled conditions: 23 +/- 0.5 degrees C, 75 +/- 5% RH and 16:8 (L:D) of photoperiod. Development time, weight of pupae, adult emergence, pre-oviposition period, fecundity and fertility were evaluated as endpoints. Larvae from glyphosate treatment molted earlier than controls. In addition, the weight of pupae, longevity, fecundity and fertility were drastically reduced in treated organisms. The reductions were more drastic when the treatments were performed at the third larval stage than as adult. The reproduction capacity of the predator was the most affected parameter and could be related to a hormonal disruption by glyphosate in the treated organisms. This work can confirm the deleterious effects of this herbicide on beneficial organisms. Also, it agrees with prior studies carried out on other predators associated to soybean pest, such as Chrysoperla externa (Neuroptera: Chrysopidae) and Alpaida veniliae (Araneae

  19. Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae of an Ecuadorian Mountain Forest Using DNA Barcoding.

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    Birthe Thormann

    Full Text Available Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient in southern Ecuador and explore their effect on species richness estimates.Based on a COI barcode data set of 674 leaf beetle specimens (Coleoptera: Chrysomelidae of 266 morphospecies from three sample sites in the Podocarpus National Park, we employed statistical parsimony analysis, distance-based clustering, GMYC- and PTP-modelling to delimit species-like units and compared them to morphology-based (parataxonomic species identifications. The four different approaches for DNA-based species delimitation revealed highly similar numbers of molecular operational taxonomic units (MOTUs (n = 284-289. Estimated total species richness was considerably higher than the sampled amount, 414 for morphospecies (Chao2 and 469-481 for the different MOTU types. Assemblages at different elevational levels (1000 vs. 2000 m had similar species numbers but a very distinct species composition for all delimitation methods. Most species were found only at one elevation while this turnover pattern was even more pronounced for DNA-based delimitation.Given the high congruence of DNA-based delimitation results, probably due to the sampling structure, our study suggests that when applied to species communities on a regionally limited level with high amount of rare species (i.e. ~50% singletons, the choice of species delimitation method can be of minor relevance for assessing species numbers and turnover in tropical insect communities. Therefore, DNA-based species delimitation is confirmed as a

  20. Interactions between imidacloprid and Metarhizium brunneum on adult Asian longhorned beetles (Anoplophora glabripennis (Motschulsky)) (Coleoptera: Cerambycidae).

    Science.gov (United States)

    Russell, Calum W; Ugine, Todd A; Hajek, Ann E

    2010-11-01

    Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), a longhorned beetle species native to Asia, has been introduced into several North American and European cities. Currently eradication and preventive measures are limited to identifying and destroying infested trees and protecting uninfested trees with trunk or soil-injections of the systemic insecticide imidacloprid. Because entomopathogenic fungi like Metarhizium brunneum Petch have been identified as virulent against these beetles we conducted several tests to determine the compatibility of the two agents in combination. Radial hyphal growth and the sporulation capacity of M. brunneum on Sabouraud dextrose agar with yeast were not significantly affected by the presence of imidacloprid. In a 2×3 factorial experiment investigating interactions between exposure to imidacloprid and M. brunneum we observed no effect of imidacloprid alone on beetle survival when beetles were given a single dose of 10 or 100 ppm compared to control insects. We observed a significant effect of exposure to M. brunneum, and a significant interaction between imidacloprid and M. brunneum representing a synergistic effect of dual treatment. Beetles exposed to the fungus alone lived significantly longer compared to insects treated with a single dose of 100 ppm imidacloprid (9.5 vs. 6.5d). Consumption of striped maple twigs by beetles exposed to imidacloprid, across concentrations, was reduced 48% compared to control insects, where as consumption by M. brunneum-exposed beetles was reduced by 16% over the first 6-days of the test period. Beetles fed 100 ppm imidacloprid consumed 32% less over the first 3d compared to beetles not exposed to imidacloprid and thereafter consumed as much as beetles not fed 100 ppm imidacloprid. M. brunneum-exposed beetles consumed significantly less food than control insects throughout the test period, and beetles treated with imidacloprid produced significantly fewer conidia compared to beetles