WorldWideScience

Sample records for cassette transport systems

  1. Automated cassette-to-cassette substrate handling system

    Science.gov (United States)

    Kraus, Joseph Arthur; Boyer, Jeremy James; Mack, Joseph; DeChellis, Michael; Koo, Michael

    2014-03-18

    An automated cassette-to-cassette substrate handling system includes a cassette storage module for storing a plurality of substrates in cassettes before and after processing. A substrate carrier storage module stores a plurality of substrate carriers. A substrate carrier loading/unloading module loads substrates from the cassette storage module onto the plurality of substrate carriers and unloads substrates from the plurality of substrate carriers to the cassette storage module. A transport mechanism transports the plurality of substrates between the cassette storage module and the plurality of substrate carriers and transports the plurality of substrate carriers between the substrate carrier loading/unloading module and a processing chamber. A vision system recognizes recesses in the plurality of substrate carriers corresponding to empty substrate positions in the substrate carrier. A processor receives data from the vision system and instructs the transport mechanism to transport substrates to positions on the substrate carrier in response to the received data.

  2. Membrane porters of ATP-binding cassette transport systems are polyphyletic.

    Science.gov (United States)

    Wang, Bin; Dukarevich, Maxim; Sun, Eric I; Yen, Ming Ren; Saier, Milton H

    2009-09-01

    The ATP-binding cassette (ABC) superfamily consists of both importers and exporters. These transporters have, by tradition, been classified according to the ATP hydrolyzing constituents, which are monophyletic. The evolutionary origins of the transmembrane porter proteins/domains are not known. Using five distinct computer programs, we here provide convincing statistical data suggesting that the transmembrane domains of ABC exporters are polyphyletic, having arisen at least three times independently. ABC1 porters arose by intragenic triplication of a primordial two-transmembrane segment (TMS)-encoding genetic element, yielding six TMS proteins. ABC2 porters arose by intragenic duplication of a dissimilar primordial three-TMS-encoding genetic element, yielding a distinctive protein family, nonhomologous to the ABC1 proteins. ABC3 porters arose by duplication of a primordial four-TMS-encoding genetic element, yielding either eight- or 10-TMS proteins. We assign each of 48 of the 50 currently recognized families of ABC exporters to one of the three evolutionarily distinct ABC types. Currently available high-resolution structural data for ABC porters are fully consistent with our findings. These results provide guides for future structural and mechanistic studies of these important transport systems.

  3. The Plasma-Facing Components Transporter (PFCT) : a Prototype System for PFC Replacement on the new ITER 2001 Cassette Mock-up

    International Nuclear Information System (INIS)

    Micciche, G.; Lorenzelli, L.; Muro, L.; Irving, M.

    2006-01-01

    The remote maintainability of the early ITER divertor cassette (based on the ITER 1998 design) was successfully proved during test campaigns carried out in the Divertor Refurbishment Platform (DRP) at the ENEA research centre at Brasimone over the period 1999-2003. Due to subsequent major modifications in the ITER divertor cassette design, the main focus over the past few years has been on the design and manufacture of the various components, devices and tools needed for refurbishment of the new ITER 2001 Divertor Cassette. The design of this new cassette differs substantially from the earlier version: in particular the shape, weight and attachment system of the Plasma Facing Components (PFC's) has been completely revised, and this also entailed a review of the procedures adopted for its refurbishment. One of the major requirements of the cassette refurbishment process is removal and replacement of the three PFC's. In the old cassette concept, target replacement was performed by means of a purpose-built '' C '' frame slung from a standard bridge crane. The 2001 cassette design precludes such handling methods for a number of reasons, notably because of the extremely tight inter-PFC clearances, and the need for controlled inclination of the target in addition to normal translational movements, both impossible with a simple Cartesian crane. To demonstrate the refurbishment feasibility operations for the new ITER Divertor 2001 cassettes, an experimental machine known as the Plasma-Facing Component Transporter (PFCT) has been designed, fabricated and commissioned in the years 2004-5. This full six degree-of-freedom system has been designed to handle payloads of up to 5 tonnes with good positional accuracy, and axes capable of very low joint velocities, including inclination of the PFC's over the range of ± 10 o in both horizontal axes, and controlled rotation about the vertical axis. Preliminary trials carried out during the commissioning phase have proved its

  4. Tape Cassette Bacteria Detection System

    Science.gov (United States)

    1973-01-01

    The design, fabrication, and testing of an automatic bacteria detection system with a zero-g capability and based on the filter-capsule approach is described. This system is intended for monitoring the sterility of regenerated water in a spacecraft. The principle of detection is based on measuring the increase in chemiluminescence produced by the action of bacterial porphyrins (i.e., catalase, cytochromes, etc.) on a luminol-hydrogen peroxide mixture. Since viable as well as nonviable organisms initiate this luminescence, viable organisms are detected by comparing the signal of an incubated water sample with an unincubated control. Higher signals for the former indicate the presence of viable organisms. System features include disposable sealed sterile capsules, each containing a filter membrane, for processing discrete water samples and a tape transport for moving these capsules through a processing sequence which involves sample concentration, nutrient addition, incubation, a 4 Molar Urea wash and reaction with luminol-hydrogen peroxide in front of a photomultiplier tube. Liquids are introduced by means of a syringe needle which pierces a rubber septum contained in the wall of the capsule. Detection thresholds obtained with this unit towards E. coli and S. marcescens assuming a 400 ml water sample are indicated.

  5. Metabolism of ATP-binding cassette drug transporter inhibitors: complicating factor for multidrug resistance.

    NARCIS (Netherlands)

    Cnubben, N.H.; Wortelboer, H.M.; Zanden, J.J. van; Rietjens, I.M.; Bladeren, P.J. van

    2005-01-01

    Membrane transport proteins belonging to the ATP-binding cassette (ABC) family of transport proteins play a central role in the defence of organisms against toxic compounds, including anticancer drugs. However, for compounds that are designed to display a toxic effect, this defence system diminishes

  6. ATP-binding Cassette (ABC) Transport System Solute-binding Protein-guided Identification of Novel d-Altritol and Galactitol Catabolic Pathways in Agrobacterium tumefaciens C58*

    Science.gov (United States)

    Wichelecki, Daniel J.; Vetting, Matthew W.; Chou, Liyushang; Al-Obaidi, Nawar; Bouvier, Jason T.; Almo, Steven C.; Gerlt, John A.

    2015-01-01

    Innovations in the discovery of the functions of uncharacterized proteins/enzymes have become increasingly important as advances in sequencing technology flood protein databases with an exponentially growing number of open reading frames. This study documents one such innovation developed by the Enzyme Function Initiative (EFI; U54GM093342), the use of solute-binding proteins for transport systems to identify novel metabolic pathways. In a previous study, this strategy was applied to the tripartite ATP-independent periplasmic transporters. Here, we apply this strategy to the ATP-binding cassette transporters and report the discovery of novel catabolic pathways for d-altritol and galactitol in Agrobacterium tumefaciens C58. These efforts resulted in the description of three novel enzymatic reactions as follows: 1) oxidation of d-altritol to d-tagatose via a dehydrogenase in Pfam family PF00107, a previously unknown reaction; 2) phosphorylation of d-tagatose to d-tagatose 6-phosphate via a kinase in Pfam family PF00294, a previously orphan EC number; and 3) epimerization of d-tagatose 6-phosphate C-4 to d-fructose 6-phosphate via a member of Pfam family PF08013, another previously unknown reaction. The epimerization reaction catalyzed by a member of PF08013 is especially noteworthy, because the functions of members of PF08013 have been unknown. These discoveries were assisted by the following two synergistic bioinformatics web tools made available by the Enzyme Function Initiative: the EFI-Enzyme Similarity Tool and the EFI-Genome Neighborhood Tool. PMID:26472925

  7. The Role of ATP-Binding Cassette Transporters in Neuro-Inflammation: Relevance for Bioactive Lipids

    OpenAIRE

    Kooij, Gijs; van Horssen, Jack; Bandaru, Veera Venkata Ratnam; Haughey, Norman J.; de Vries, Helga E.

    2012-01-01

    ATP-binding cassette (ABC) transporters are highly expressed by brain endothelial cells that form the blood-brain barrier (BBB). These efflux pumps play an important role in maintaining brain homeostasis as they actively hinder the entry of unwanted blood-derived compounds into the central nervous system (CNS). Consequently, their high activity at the BBB has been a major hurdle for the treatment of several brain diseases, as they prevent numerous drugs to reach their site of action within th...

  8. ATP-binding cassette transporters in reproduction: a new frontier

    Science.gov (United States)

    Bloise, E.; Ortiga-Carvalho, T.M.; Reis, F.M.; Lye, S.J.; Gibb, W.; Matthews, S.G.

    2016-01-01

    BACKGROUND The transmembrane ATP-binding cassette (ABC) transporters actively efflux an array of clinically relevant compounds across biological barriers, and modulate biodistribution of many physiological and pharmacological factors. To date, over 48 ABC transporters have been identified and shown to be directly and indirectly involved in peri-implantation events and fetal/placental development. They efflux cholesterol, steroid hormones, vitamins, cytokines, chemokines, prostaglandins, diverse xenobiotics and environmental toxins, playing a critical role in regulating drug disposition, immunological responses and lipid trafficking, as well as preventing fetal accumulation of drugs and environmental toxins. METHODS This review examines ABC transporters as important mediators of placental barrier functions and key reproductive processes. Expression, localization and function of all identified ABC transporters were systematically reviewed using PubMed and Google Scholar websites to identify relevant studies examining ABC transporters in reproductive tissues in physiological and pathophysiological states. Only reports written in English were incorporated with no restriction on year of publication. While a major focus has been placed on the human, extensive evidence from animal studies is utilized to describe current understanding of the regulation and function of ABC transporters relevant to human reproduction. RESULTS ABC transporters are modulators of steroidogenesis, fertilization, implantation, nutrient transport and immunological responses, and function as ‘gatekeepers’ at various barrier sites (i.e. blood-testes barrier and placenta) against potentially harmful xenobiotic factors, including drugs and environmental toxins. These roles appear to be species dependent and change as a function of gestation and development. The best-described ABC transporters in reproductive tissues (primarily in the placenta) are the multidrug transporters p-glycoprotein and

  9. Small substrate transport and mechanism of a molybdate ATP binding cassette transporter in a lipid environment.

    Science.gov (United States)

    Rice, Austin J; Harrison, Alistair; Alvarez, Frances J D; Davidson, Amy L; Pinkett, Heather W

    2014-05-23

    Embedded in the plasma membrane of all bacteria, ATP binding cassette (ABC) importers facilitate the uptake of several vital nutrients and cofactors. The ABC transporter, MolBC-A, imports molybdate by passing substrate from the binding protein MolA to a membrane-spanning translocation pathway of MolB. To understand the mechanism of transport in the biological membrane as a whole, the effects of the lipid bilayer on transport needed to be addressed. Continuous wave-electron paramagnetic resonance and in vivo molybdate uptake studies were used to test the impact of the lipid environment on the mechanism and function of MolBC-A. Working with the bacterium Haemophilus influenzae, we found that MolBC-A functions as a low affinity molybdate transporter in its native environment. In periods of high extracellular molybdate concentration, H. influenzae makes use of parallel molybdate transport systems (MolBC-A and ModBC-A) to take up a greater amount of molybdate than a strain with ModBC-A alone. In addition, the movement of the translocation pathway in response to nucleotide binding and hydrolysis in a lipid environment is conserved when compared with in-detergent analysis. However, electron paramagnetic resonance spectroscopy indicates that a lipid environment restricts the flexibility of the MolBC translocation pathway. By combining continuous wave-electron paramagnetic resonance spectroscopy and substrate uptake studies, we reveal details of molybdate transport and the logistics of uptake systems that employ multiple transporters for the same substrate, offering insight into the mechanisms of nutrient uptake in bacteria. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  10. ATP-binding cassette (ABC) transporters in normal and pathological lung

    NARCIS (Netherlands)

    van der Deen, M; de Vries, EGE; Timens, W; Scheper, RJ; Timmer-Bosscha, H; Postma, DS

    2005-01-01

    ATP-binding cassette ( ABC) transporters are a family of transmembrane proteins that can transport a wide variety of substrates across biological membranes in an energy-dependent manner. Many ABC transporters such as P-glycoprotein ( P-gp), multidrug resistance-associated protein 1 ( MRP1) and

  11. ATP-Binding Cassette Systems of Brucella

    Directory of Open Access Journals (Sweden)

    Dominic C. Jenner

    2009-01-01

    Full Text Available Brucellosis is a prevalent zoonotic disease and is endemic in the Middle East, South America, and other areas of the world. In this study, complete inventories of putative functional ABC systems of five Brucella species have been compiled and compared. ABC systems of Brucella melitensis 16M, Brucella abortus 9-941, Brucella canis RM6/66, Brucella suis 1330, and Brucella ovis 63/290 were identified and aligned. High numbers of ABC systems, particularly nutrient importers, were found in all Brucella species. However, differences in the total numbers of ABC systems were identified (B. melitensis, 79; B. suis, 72; B. abortus 64; B. canis, 74; B. ovis, 59 as well as specific differences in the functional ABC systems of the Brucella species. Since B. ovis is not known to cause human brucellosis, functional ABC systems absent in the B. ovis genome may represent virulence factors in human brucellosis.

  12. Multidrug ATP-binding cassette transporters are essential for hepatic development of Plasmodium sporozoites

    NARCIS (Netherlands)

    Rijpma, S.R.; Velden, M. van der; Gonzalez-Pons, M.; Annoura, T.; Schaijk, B.C.L. van; Gemert, G.J.A. van; Heuvel, J.M.W. van den; Ramesar, J.; Chevalley-Maurel, S.; Ploemen, I.H.; Khan, S.M.; Franetich, J.F.; Mazier, D.; Wilt, J.H.W. de; Serrano, A.E.; Russel, F.G.; Janse, C.J.; Sauerwein, R.W.; Koenderink, J.B.; Franke-Fayard, B.M.

    2016-01-01

    Multidrug resistance-associated proteins (MRPs) belong to the C-family of ATP-binding cassette (ABC) transport proteins and are known to transport a variety of physiologically important compounds and to be involved in the extrusion of pharmaceuticals. Rodent malaria parasites encode a single ABC

  13. ATP Binding cassette transporter gene expression in rat liver progenitor cells

    NARCIS (Netherlands)

    Ros, J.E.; Roskams, T.A.D.; Geuken, M.; Havinga, R.; Splinter, P.L.; Petersen, B.E.; LaRusso, N.F.; Kolk, van der D.M.; Kuipers, F.; Faber, K.N.; Müller, M.R.; Jansen, P.L.M.

    2003-01-01

    Background and aim: Liver regeneration after severe liver damage depends in part on proliferation and differentiation of hepatic progenitor cells (HPCs). Under these conditions they must be able to withstand the toxic milieu of the damaged liver. ATP binding cassette (ABC) transporters are

  14. ATP binding cassette transporter gene expression in rat liver progenitor cells

    NARCIS (Netherlands)

    Ros, J. E.; Roskams, T. A. D.; Geuken, M.; Havinga, R.; Splinter, P. L.; Petersen, B. E.; LaRusso, N. F.; van der Kolk, D. M.; Kuipers, F.; Faber, K. N.; Müller, M.; Jansen, P. L. M.

    2003-01-01

    BACKGROUND AND AIM: Liver regeneration after severe liver damage depends in part on proliferation and differentiation of hepatic progenitor cells (HPCs). Under these conditions they must be able to withstand the toxic milieu of the damaged liver. ATP binding cassette (ABC) transporters are

  15. ATP binding cassette transporter gene expression in rat liver progenitor cells

    NARCIS (Netherlands)

    Ros, J.E.; Roskams, TAD; Geuken, M; Havinga, R; Splinter, PL; Petersen, BE; LaRusso, NF; van der Kolk, D.M.; Kuipers, F; Faber, KN; Muller, M; Jansen, PLM

    Background and aim: Liver regeneration after severe liver damage depends in part on proliferation and differentiation of hepatic progenitor cells (HPCs). Under these conditions they must be able to withstand the toxic milieu of the damaged liver. ATP binding cassette (ABC) transporters are

  16. Structure-function analysis of peroxisomal ATP-binding cassette transporters using chimeric dimers

    NARCIS (Netherlands)

    Geillon, Flore; Gondcaille, Catherine; Charbonnier, Soëli; van Roermund, Carlo W.; Lopez, Tatiana E.; Dias, Alexandre M. M.; Pais de Barros, Jean-Paul; Arnould, Christine; Wanders, Ronald J.; Trompier, Doriane; Savary, Stéphane

    2014-01-01

    ABCD1 and ABCD2 are two closely related ATP-binding cassette half-transporters predicted to homodimerize and form peroxisomal importers for fatty acyl-CoAs. Available evidence has shown that ABCD1 and ABCD2 display a distinct but overlapping substrate specificity, although much remains to be learned

  17. The Role of ATP-Binding Cassette Transporters in Neuro-Inflammation: Relevance for Bioactive Lipids

    NARCIS (Netherlands)

    Kooij, G.; van Horssen, J.; Bandaru, V.V.R.; Haughey, N.J.; de Vries, H.E.

    2012-01-01

    ATP-binding cassette (ABC) transporters are highly expressed by brain endothelial cells that form the blood-brain barrier (BBB). These efflux pumps play an important role in maintaining brain homeostasis as they actively hinder the entry of unwanted blood-derived compounds into the central nervous

  18. Serum albumin promotes ATP-binding cassette transporter-dependent sterol uptake in yeast

    DEFF Research Database (Denmark)

    Marek, Magdalena; Silvestro, Daniele; Fredslund, Maria D.

    2014-01-01

    Sterol uptake in fungi is a multistep process that involves interaction between external sterols and the cell wall, incorporation of sterol molecules into the plasma membrane, and subsequent integration into intracellular membranes for turnover. ATP-binding cassette (ABC) transporters have been...

  19. The role of ATP-binding cassette transporters in neuro-inflammation: relevance for bioactive lipids

    Directory of Open Access Journals (Sweden)

    Gijs eKooij

    2012-04-01

    Full Text Available ATP-binding cassette (ABC transporters are highly expressed by brain endothelial cells that form the blood-brain barrier (BBB. These efflux pumps play an important role in maintaining brain homeostasis as they actively hinder the entry of unwanted blood-derived compounds into the central nervous system (CNS. Consequently, their high activity at the BBB has been a major hurdle for the treatment of several brain diseases, as they prevent numerous drugs to reach their site of action within the brain. Importantly, recent data indicate that endogenous substrates for ABC transporters may include inflammatory mediators, such as prostaglandins, leukotrienes, cytokines, chemokines and bioactive lipids, suggesting a potential role for ABC transporters in immunological responses, and more specifically in inflammatory brain disorders, such as multiple sclerosis (MS. In this review, we will give a comprehensive overview of recent findings that illustrate this novel role for ABC transporters in neuro-inflammatory processes. Moreover, we will provide first insights into underlying mechanisms and focus on the importance for bioactive lipids, in particular platelet-activating factor (PAF, herein. A thorough understanding of these events may form the basis for the development for selective treatment modalities to dampen the neuro-inflammatory attack in MS and thereby reducing tissue damage.

  20. Opioid transport by ATP-binding cassette transporters at the blood-brain barrier: implications for neuropsychopharmacology.

    Science.gov (United States)

    Tournier, Nicolas; Declèves, Xavier; Saubaméa, Bruno; Scherrmann, Jean-Michel; Cisternino, Salvatore

    2011-01-01

    Some of the ATP-binding cassette (ABC) transporters like P-glycoprotein (P-gp; ABCB1, MDR1), BCRP (ABCG2) and MRPs (ABCCs) that are present at the blood-brain barrier (BBB) influence the brain pharmacokinetics (PK) of their substrates by restricting their uptake or enhancing their clearance from the brain into the blood, which has consequences for their CNS pharmacodynamics (PD). Opioid drugs have been invaluable tools for understanding the PK-PD relationships of these ABC-transporters. The effects of morphine, methadone and loperamide on the CNS are modulated by P-gp. This review examines the ways in which other opioid drugs and some of their active metabolites interact with ABC transporters and suggests new mechanisms that may be involved in the variability of the response of the CNS to these drugs like carrier-mediated system belonging to the solute carrier (SLC) superfamily. Exposure to opioids may also alter the expression of ABC transporters. P-gp can be overproduced during morphine treatment, suggesting that the drug has a direct or, more likely, an indirect action. Variations in cerebral neurotransmitters during exposure to opioids and the release of cytokines during pain could be new endogenous stimuli affecting transporter synthesis. This review concludes with an analysis of the pharmacotherapeutic and clinical impacts of the interactions between ABC transporters and opioids.

  1. Functional Analysis of an ATP-Binding Cassette Transporter Gene in Botrytis cinerea by Gene Disruption

    OpenAIRE

    Masami, NAKAJIMA; Junko, SUZUKI; Takehiko, HOSAKA; Tadaaki, HIBI; Katsumi, AKUTSU; School of Agriculture, Ibaraki University; School of Agriculture, Ibaraki University; School of Agriculture, Ibaraki University; Department of Agriculture and Environmental Biology, The University of Tokyo; School of Agriculture, Ibaraki University

    2001-01-01

    The BMR1 gene encoding an ABC transporter was cloned from Botrytis cinerea. To examine the function of BMR1 in B.cinerea, we isolated BMR1-deficient mutants after gene disruption. Disruption vector pBcDF4 was constructed by replacing the BMR1-coding region with a hygromycin B phosphotransferase gene(hph)cassette. The BMR1 disruptants had an increased sensitivity to polyoxin and iprobenfos. Polyoxin and iprobenfos, structurally unrelated compounds, may therefore be substrates of BMR1.

  2. Mouse ATP-Binding Cassette (ABC) Transporters Conferring Multi-Drug Resistance

    Science.gov (United States)

    Shuaizhang, L I; Zhang, Wen; Yin, Xuejiao; Xing, Shilai; Xie, Qunhui; Cao, Zhengyu; Zhao, Bin

    2015-04-28

    The ABC (ATP-binding cassette) transporter is one of the largest and most ancient protein families with members functioning from protozoa to human. The resistance of cancer and tumor cells to anticancer drugs is due to the over-expression of some ABC transporters, which may finally lead to chemotherapy failure. The mouse ABC transporters are classified into seven subfamilies by phylogenetic analysis. The mouse ABC transporter gene, alias, chromosomal location and function have been determined. Within the ABC super-family, the MDR transporters (Abcb1, Abcc1, Abcg2) in mouse models have been proved to be valuable to investigate the biochemistry and physiological functions. This review concentrates on the multidrug resistance of mouse ABC transporters in cancer and tumor cells.

  3. A two-cassette reporter system for assessing target gene translation and target gene product inclusion body formation

    DEFF Research Database (Denmark)

    2016-01-01

    The present invention relates to a dual cassette reporter system capable of assessing target gene translation and target gene product folding. The present invention further relates to vectors and host cells comprising the dual cassette reporter system. In addition the invention relates to the use...... of the dual cassette reporter system for assessing target gene translation and target gene product folding....

  4. A clinical trial of a rare earth screen/film system in a periapical cassette

    International Nuclear Information System (INIS)

    Kogon, S.L.; Stephens, R.G.; Reid, J.A.; Lubus, N.J.

    1984-01-01

    In a clinical trial, a slow rare earth screen/film system (Siemens Titan 2D/Kodak XG) was used to obtain intraoral radiographs at conventional monitoring stages in endodontic treatment. The screen film image proved to be an effective substitute for the direct-exposure Ultraspeed periapical film. The intraoral cassettes, designed and fabricated for the study, were an adaptation of the flexible, vacuum-sealed cassettes used in mammography. It is believed that when a practicable periapical cassette is manufactured, many additional indications for the system are probable. Major reductions in patient exposure of at least 85% to 90% per periapical film would be effected

  5. Efficient purification and reconstitution of ATP binding cassette transporter B6 (ABCB6) for functional and structural studies.

    Science.gov (United States)

    Chavan, Hemantkumar; Khan, Mohiuddin Md Taimur; Tegos, George; Krishnamurthy, Partha

    2013-08-02

    The mitochondrial ATP binding cassette transporter ABCB6 has been associated with a broad range of physiological functions, including growth and development, therapy-related drug resistance, and the new blood group system Langereis. ABCB6 has been proposed to regulate heme synthesis by shuttling coproporphyrinogen III from the cytoplasm into the mitochondria. However, direct functional information of the transport complex is not known. To understand the role of ABCB6 in mitochondrial transport, we developed an in vitro system with pure and active protein. ABCB6 overexpressed in HEK293 cells was solubilized from mitochondrial membranes and purified to homogeneity. Purified ABCB6 showed a high binding affinity for MgATP (Kd = 0.18 μM) and an ATPase activity with a Km of 0.99 mM. Reconstitution of ABCB6 into liposomes allowed biochemical characterization of the ATPase including (i) substrate-stimulated ATPase activity, (ii) transport kinetics of its proposed endogenous substrate coproporphyrinogen III, and (iii) transport kinetics of substrates identified using a high throughput screening assay. Mutagenesis of the conserved lysine to alanine (K629A) in the Walker A motif abolished ATP hydrolysis and substrate transport. These results suggest a direct interaction between mitochondrial ABCB6 and its transport substrates that is critical for the activity of the transporter. Furthermore, the simple immunoaffinity purification of ABCB6 to near homogeneity and efficient reconstitution of ABCB6 into liposomes might provide the basis for future studies on the structure/function of ABCB6.

  6. Functional analysis of the ATP-binding cassette (ABC) transporter gene family of Tribolium castaneum.

    Science.gov (United States)

    Broehan, Gunnar; Kroeger, Tobias; Lorenzen, Marcé; Merzendorfer, Hans

    2013-01-16

    The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. Most are integral membrane proteins that transport a broad spectrum of substrates across lipid membranes. In insects, ABC transporters are of special interest because of their role in insecticide resistance. We have identified 73 ABC transporter genes in the genome of T. castaneum, which group into eight subfamilies (ABCA-H). This coleopteran ABC family is significantly larger than those reported for insects in other taxonomic groups. Phylogenetic analysis revealed that this increase is due to gene expansion within a single clade of subfamily ABCC. We performed an RNA interference (RNAi) screen to study the function of ABC transporters during development. In ten cases, injection of double-stranded RNA (dsRNA) into larvae caused developmental phenotypes, which included growth arrest and localized melanization, eye pigmentation defects, abnormal cuticle formation, egg-laying and egg-hatching defects, and mortality due to abortive molting and desiccation. Some of the ABC transporters we studied in closer detail to examine their role in lipid, ecdysteroid and eye pigment transport. The results from our study provide new insights into the physiological function of ABC transporters in T. castaneum, and may help to establish new target sites for insect control.

  7. The Yeast Plasma Membrane ATP Binding Cassette (ABC) Transporter Aus1

    Science.gov (United States)

    Marek, Magdalena; Milles, Sigrid; Schreiber, Gabriele; Daleke, David L.; Dittmar, Gunnar; Herrmann, Andreas; Müller, Peter; Pomorski, Thomas Günther

    2011-01-01

    The ATP binding cassette (ABC) transporter Aus1 is expressed under anaerobic growth conditions at the plasma membrane of the yeast Saccharomyces cerevisiae and is required for sterol uptake. These observations suggest that Aus1 promotes the translocation of sterols across membranes, but the precise transport mechanism has yet to be identified. In this study, an extraction and purification procedure was developed to characterize the Aus1 transporter. The detergent-solubilized protein was able to bind and hydrolyze ATP. Mutagenesis of the conserved lysine to methionine in the Walker A motif abolished ATP hydrolysis. Likewise, ATP hydrolysis was inhibited by classical inhibitors of ABC transporters. Upon reconstitution into proteoliposomes, the ATPase activity of Aus1 was specifically stimulated by phosphatidylserine (PS) in a stereoselective manner. We also found that Aus1-dependent sterol uptake, but not Aus1 expression and trafficking to the plasma membrane, was affected by changes in cellular PS levels. These results suggest a direct interaction between Aus1 and PS that is critical for the activity of the transporter. PMID:21521689

  8. Multidrug ATP-binding cassette transporters are essential for hepatic development of Plasmodium sporozoites.

    Science.gov (United States)

    Rijpma, Sanna R; van der Velden, Maarten; González-Pons, Maria; Annoura, Takeshi; van Schaijk, Ben C L; van Gemert, Geert-Jan; van den Heuvel, Jeroen J M W; Ramesar, Jai; Chevalley-Maurel, Severine; Ploemen, Ivo H; Khan, Shahid M; Franetich, Jean-Francois; Mazier, Dominique; de Wilt, Johannes H W; Serrano, Adelfa E; Russel, Frans G M; Janse, Chris J; Sauerwein, Robert W; Koenderink, Jan B; Franke-Fayard, Blandine M

    2016-03-01

    Multidrug resistance-associated proteins (MRPs) belong to the C-family of ATP-binding cassette (ABC) transport proteins and are known to transport a variety of physiologically important compounds and to be involved in the extrusion of pharmaceuticals. Rodent malaria parasites encode a single ABC transporter subfamily C protein, whereas human parasites encode two: MRP1 and MRP2. Although associated with drug resistance, their biological function and substrates remain unknown. To elucidate the role of MRP throughout the parasite life cycle, Plasmodium berghei and Plasmodium falciparum mutants lacking MRP expression were generated. P. berghei mutants lacking expression of the single MRP as well as P. falciparum mutants lacking MRP1, MRP2 or both proteins have similar blood stage growth kinetics and drug-sensitivity profiles as wild type parasites. We show that MRP1-deficient parasites readily invade primary human hepatocytes and develop into mature liver stages. In contrast, both P. falciparum MRP2-deficient parasites and P. berghei mutants lacking MRP protein expression abort in mid to late liver stage development, failing to produce mature liver stages. The combined P. berghei and P. falciparum data are the first demonstration of a critical role of an ABC transporter during Plasmodium liver stage development. © 2015 John Wiley & Sons Ltd.

  9. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells.

    Directory of Open Access Journals (Sweden)

    Flavio Alves Lara

    Full Text Available In ticks, the digestion of blood occurs intracellularly and proteolytic digestion of hemoglobin takes place in a dedicated type of lysosome, the digest vesicle, followed by transfer of the heme moiety of hemoglobin to a specialized organelle that accumulates large heme aggregates, called hemosomes. In the present work, we studied the uptake of fluorescent metalloporphyrins, used as heme analogs, and amitraz, one of the most regularly used acaricides to control cattle tick infestations, by Rhipicephalus (Boophilus microplus midgut cells. Both compounds were taken up by midgut cells in vitro and accumulated inside the hemosomes. Transport of both molecules was sensitive to cyclosporine A (CsA, a well-known inhibitor of ATP binding cassette (ABC transporters. Rhodamine 123, a fluorescent probe that is also a recognized ABC substrate, was similarly directed to the hemosome in a CsA-sensitive manner. Using an antibody against conserved domain of PgP-1-type ABC transporter, we were able to immunolocalize PgP-1 in the digest vesicle membranes. Comparison between two R. microplus strains that were resistant and susceptible to amitraz revealed that the resistant strain detoxified both amitraz and Sn-Pp IX more efficiently than the susceptible strain, a process that was also sensitive to CsA. A transcript containing an ABC transporter signature exhibited 2.5-fold increased expression in the amitraz-resistant strain when compared with the susceptible strain. RNAi-induced down-regulation of this ABC transporter led to the accumulation of metalloporphyrin in the digestive vacuole, interrupting heme traffic to the hemosome. This evidence further confirms that this transcript codes for a heme transporter. This is the first report of heme transport in a blood-feeding organism. While the primary physiological function of the hemosome is to detoxify heme and attenuate its toxicity, we suggest that the use of this acaricide detoxification pathway by ticks may

  10. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells

    Science.gov (United States)

    Lara, Flavio Alves; Pohl, Paula C.; Gandara, Ana Caroline; Ferreira, Jessica da Silva; Nascimento-Silva, Maria Clara; Bechara, Gervásio Henrique; Sorgine, Marcos H. F.; Almeida, Igor C.; Vaz, Itabajara da Silva; Oliveira, Pedro L.

    2015-01-01

    In ticks, the digestion of blood occurs intracellularly and proteolytic digestion of hemoglobin takes place in a dedicated type of lysosome, the digest vesicle, followed by transfer of the heme moiety of hemoglobin to a specialized organelle that accumulates large heme aggregates, called hemosomes. In the present work, we studied the uptake of fluorescent metalloporphyrins, used as heme analogs, and amitraz, one of the most regularly used acaricides to control cattle tick infestations, by Rhipicephalus (Boophilus) microplus midgut cells. Both compounds were taken up by midgut cells in vitro and accumulated inside the hemosomes. Transport of both molecules was sensitive to cyclosporine A (CsA), a well-known inhibitor of ATP binding cassette (ABC) transporters. Rhodamine 123, a fluorescent probe that is also a recognized ABC substrate, was similarly directed to the hemosome in a CsA-sensitive manner. Using an antibody against conserved domain of PgP-1-type ABC transporter, we were able to immunolocalize PgP-1 in the digest vesicle membranes. Comparison between two R. microplus strains that were resistant and susceptible to amitraz revealed that the resistant strain detoxified both amitraz and Sn-Pp IX more efficiently than the susceptible strain, a process that was also sensitive to CsA. A transcript containing an ABC transporter signature exhibited 2.5-fold increased expression in the amitraz-resistant strain when compared with the susceptible strain. RNAi-induced down-regulation of this ABC transporter led to the accumulation of metalloporphyrin in the digestive vacuole, interrupting heme traffic to the hemosome. This evidence further confirms that this transcript codes for a heme transporter. This is the first report of heme transport in a blood-feeding organism. While the primary physiological function of the hemosome is to detoxify heme and attenuate its toxicity, we suggest that the use of this acaricide detoxification pathway by ticks may represent a new

  11. CMOS cassette for digital upgrade of film-based mammography systems

    Science.gov (United States)

    Baysal, Mehmet A.; Toker, Emre

    2006-03-01

    While full-field digital mammography (FFDM) technology is gaining clinical acceptance, the overwhelming majority (96%) of the installed base of mammography systems are conventional film-screen (FSM) systems. A high performance, and economical digital cassette based product to conveniently upgrade FSM systems to FFDM would accelerate the adoption of FFDM, and make the clinical and technical advantages of FFDM available to a larger population of women. The planned FFDM cassette is based on our commercial Digital Radiography (DR) cassette for 10 cm x 10 cm field-of-view spot imaging and specimen radiography, utilizing a 150 micron columnar CsI(Tl) scintillator and 48 micron active-pixel CMOS sensor modules. Unlike a Computer Radiography (CR) cassette, which requires an external digitizer, our DR cassette transfers acquired images to a display workstation within approximately 5 seconds of exposure, greatly enhancing patient flow. We will present the physical performance of our prototype system against other FFDM systems in clinical use today, using established objective criteria such as the Modulation Transfer Function (MTF), Detective Quantum Efficiency (DQE), and subjective criteria, such as a contrast-detail (CD-MAM) observer performance study. Driven by the strong demand from the computer industry, CMOS technology is one of the lowest cost, and the most readily accessible technologies available for FFDM today. Recent popular use of CMOS imagers in high-end consumer cameras have also resulted in significant advances in the imaging performance of CMOS sensors against rivaling CCD sensors. This study promises to take advantage of these unique features to develop the first CMOS based FFDM upgrade cassette.

  12. A novel flow cytometric HTS assay reveals functional modulators of ATP binding cassette transporter ABCB6.

    Science.gov (United States)

    Polireddy, Kishore; Khan, Mohiuddin Md Taimur; Chavan, Hemantkumar; Young, Susan; Ma, Xiaochao; Waller, Anna; Garcia, Matthew; Perez, Dominique; Chavez, Stephanie; Strouse, Jacob J; Haynes, Mark K; Bologa, Cristian G; Oprea, Tudor I; Tegos, George P; Sklar, Larry A; Krishnamurthy, Partha

    2012-01-01

    ABCB6 is a member of the adenosine triphosphate (ATP)-binding cassette family of transporter proteins that is increasingly recognized as a relevant physiological and therapeutic target. Evaluation of modulators of ABCB6 activity would pave the way toward a more complete understanding of the significance of this transport process in tumor cell growth, proliferation and therapy-related drug resistance. In addition, this effort would improve our understanding of the function of ABCB6 in normal physiology with respect to heme biosynthesis, and cellular adaptation to metabolic demand and stress responses. To search for modulators of ABCB6, we developed a novel cell-based approach that, in combination with flow cytometric high-throughput screening (HTS), can be used to identify functional modulators of ABCB6. Accumulation of protoporphyrin, a fluorescent molecule, in wild-type ABCB6 expressing K562 cells, forms the basis of the HTS assay. Screening the Prestwick Chemical Library employing the HTS assay identified four compounds, benzethonium chloride, verteporfin, tomatine hydrochloride and piperlongumine, that reduced ABCB6 mediated cellular porphyrin levels. Validation of the identified compounds employing the hemin-agarose affinity chromatography and mitochondrial transport assays demonstrated that three out of the four compounds were capable of inhibiting ABCB6 mediated hemin transport into isolated mitochondria. However, only verteporfin and tomatine hydrochloride inhibited ABCB6's ability to compete with hemin as an ABCB6 substrate. This assay is therefore sensitive, robust, and suitable for automation in a high-throughput environment as demonstrated by our identification of selective functional modulators of ABCB6. Application of this assay to other libraries of synthetic compounds and natural products is expected to identify novel modulators of ABCB6 activity.

  13. A novel flow cytometric HTS assay reveals functional modulators of ATP binding cassette transporter ABCB6.

    Directory of Open Access Journals (Sweden)

    Kishore Polireddy

    Full Text Available ABCB6 is a member of the adenosine triphosphate (ATP-binding cassette family of transporter proteins that is increasingly recognized as a relevant physiological and therapeutic target. Evaluation of modulators of ABCB6 activity would pave the way toward a more complete understanding of the significance of this transport process in tumor cell growth, proliferation and therapy-related drug resistance. In addition, this effort would improve our understanding of the function of ABCB6 in normal physiology with respect to heme biosynthesis, and cellular adaptation to metabolic demand and stress responses. To search for modulators of ABCB6, we developed a novel cell-based approach that, in combination with flow cytometric high-throughput screening (HTS, can be used to identify functional modulators of ABCB6. Accumulation of protoporphyrin, a fluorescent molecule, in wild-type ABCB6 expressing K562 cells, forms the basis of the HTS assay. Screening the Prestwick Chemical Library employing the HTS assay identified four compounds, benzethonium chloride, verteporfin, tomatine hydrochloride and piperlongumine, that reduced ABCB6 mediated cellular porphyrin levels. Validation of the identified compounds employing the hemin-agarose affinity chromatography and mitochondrial transport assays demonstrated that three out of the four compounds were capable of inhibiting ABCB6 mediated hemin transport into isolated mitochondria. However, only verteporfin and tomatine hydrochloride inhibited ABCB6's ability to compete with hemin as an ABCB6 substrate. This assay is therefore sensitive, robust, and suitable for automation in a high-throughput environment as demonstrated by our identification of selective functional modulators of ABCB6. Application of this assay to other libraries of synthetic compounds and natural products is expected to identify novel modulators of ABCB6 activity.

  14. Bilirubin Decreases Macrophage Cholesterol Efflux and ATP-Binding Cassette Transporter A1 Protein Expression.

    Science.gov (United States)

    Wang, Dongdong; Tosevska, Anela; Heiß, Elke H; Ladurner, Angela; Mölzer, Christine; Wallner, Marlies; Bulmer, Andrew; Wagner, Karl-Heinz; Dirsch, Verena M; Atanasov, Atanas G

    2017-04-28

    Mild but chronically elevated circulating unconjugated bilirubin is associated with reduced total and low-density lipoprotein cholesterol concentration, which is associated with reduced cardiovascular disease risk. We aimed to investigate whether unconjugated bilirubin influences macrophage cholesterol efflux, as a potential mechanism for the altered circulating lipoprotein concentrations observed in hyperbilirubinemic individuals. Cholesterol efflux from THP-1 macrophages was assessed using plasma obtained from normo- and hyperbilirubinemic (Gilbert syndrome) humans (n=60 per group) or (heterozygote/homozygote Gunn) rats (n=20 per group) as an acceptor. Hyperbilirubinemic plasma from patients with Gilbert syndrome and Gunn rats induced significantly reduced cholesterol efflux compared with normobilirubinemic plasma. Unconjugated bilirubin (3-17.1 μmol/L) exogenously added to plasma- or apolipoprotein A1-supplemented media also decreased macrophage cholesterol efflux in a concentration- and time-dependent manner. We also showed reduced protein expression of the ATP-binding cassette transporter A1 (ABCA1), a transmembrane cholesterol transporter involved in apolipoprotein A1-mediated cholesterol efflux, in THP-1 macrophages treated with unconjugated bilirubin and in peripheral blood mononuclear cells obtained from hyperbilirubinemic individuals. Furthermore, we demonstrated that bilirubin accelerates the degradation rate of the ABCA1 protein in THP-1 macrophages. Cholesterol efflux from THP-1 macrophages is decreased in the presence of plasma obtained from humans and rats with mild hyperbilirubinemia. A direct effect of unconjugated bilirubin on cholesterol efflux was demonstrated and is associated with decreased ABCA1 protein expression. These data improve our knowledge concerning bilirubin's impact on cholesterol transport and represent an important advancement in our understanding of bilirubin's role in cardiovascular disease. © 2017 The Authors. Published on

  15. Association of ATP-Binding Cassette Transporter A1 Gene Polymorphisms in Type 2 Diabetes Mellitus among Malaysians

    OpenAIRE

    Haghvirdizadeh, Polin; Ramachandran, Vasudevan; Etemad, Ali; Heidari, Farzad; Ghodsian, Nooshin; Bin Ismail, Norzian; Ismail, Patimah

    2015-01-01

    Background. Type 2 diabetes mellitus (T2DM) is a complex polygenic disorder characterized by impaired insulin resistance, insulin secretion, and dysregulation of lipid and protein metabolism with environmental and genetic factors. ATP-binding cassette transporter A1 (ABCA1) gene polymorphisms are reported as the one of the genetic risk factors for T2DM in various populations with conflicting results. This study was conducted based on PCR-HRM to determine the frequency of ABCA1 gene by rs22308...

  16. Conceptual design of divertor cassette handling by remote handling system of JT-60SA

    International Nuclear Information System (INIS)

    Hayashi, Takao; Sakurai, Shinji; Masaki, Kei; Tamai, Hiroshi; Yoshida, Kiyoshi; Matsukawa, Makoto

    2008-01-01

    The JT-60SA aims to contribute and supplement ITER toward demonstration fusion reactor based on tokamak concept. One of the features of JT-60SA is its high power long pulse heating, causing the large annual neutron fluence. Because the expected dose rate at the vacuum vessel (VV) may exceed 1 mSv/hr after 10 years operation and three month cooling, the human access inside the VV is restricted. Therefore a remote handling (RH) system is necessary for the maintenance and repair of in-vessel components. This paper described the RH system of JT-60SA, especially the expansion of the RH rail and exchange of the divertor cassettes. The RH rail is divided into nine and three-point mounting. The nine sections can cover 225 degrees in toroidal direction. A divertor cassette, which is 10 degrees wide in toroidal direction and weighs 500 kg itself due to the limitations of port width and handling weight, can be exchanged by heavy weight manipulator (HWM). The HWM brings the divertor cassette to the front of the other RH port, which is used for supporting the rail and/or carrying in and out equipments. Then another RH device receives and brings out the cassette by a pallet installed from outside the VV. (author)

  17. Molecular Events Involved in a Single Cycle of Ligand Transfer from an ATP Binding Cassette Transporter, LolCDE, to a Molecular Chaperone, LolA*

    OpenAIRE

    Taniguchi, Naohiro; Tokuda, Hajime

    2008-01-01

    An ATP binding cassette transporter LolCDE complex releases lipoproteins from the inner membrane of Escherichia coli in an ATP-dependent manner, leading to the formation of a complex between a lipoprotein and a periplasmic chaperone, LolA. LolA is proposed to undergo a conformational change upon the lipoprotein binding. The lipoprotein is then transferred from the LolA-lipoprotein complex to the outer membrane via LolB. Unlike most ATP binding cassette transporters med...

  18. Cloning, characterization and tissue distribution of the rat ATP-binding cassette (ABC) transporter ABC2/ABCA2.

    OpenAIRE

    Zhao, L X; Zhou, C J; Tanaka, A; Nakata, M; Hirabayashi, T; Amachi, T; Shioda, S; Ueda, K; Inagaki, N

    2000-01-01

    The ABC1 (ABCA) subfamily of the ATP-binding cassette (ABC) transporter superfamily has a structural feature that distinguishes it from other ABC transporters. Here we report the cloning, molecular characterization and tissue distribution of ABC2/ABCA2, which belongs to the ABC1 subfamily. Rat ABC2 is a protein of 2434 amino acids that has 44.5%, 40.0% and 40.8% identity with mouse ABC1/ABCA1, human ABC3/ABCA3 and human ABCR/ABCA4 respectively. Immunoblot analysis showed that proteins of 260 ...

  19. Film sheet cassette

    International Nuclear Information System (INIS)

    1981-01-01

    A novel film sheet cassette is described for handling CAT photographic films under daylight conditions and facilitating their imaging. A detailed description of the design and operation of the cassette is given together with appropriate illustrations. The resulting cassette is a low-cost unit which is easily constructed and yet provides a sure light-tight seal for the interior contents of the cassette. The individual resilient fingers on the light-trap permit the ready removal of the slide plate for taking pictures. The stippled, non-electrostatic surface of the pressure plate ensures an air layer and free slidability of the film for removal and withdrawal of the film sheet. The advantage of the daylight system is that a darkroom need not be used for inserting and removing the film in and out of the cassette resulting in a considerable time saving. (U.K.)

  20. Role of ATP-binding cassette and solute carrier transporters in erlotinib CNS penetration and intracellular accumulation.

    Science.gov (United States)

    Elmeliegy, Mohamed A; Carcaboso, Angel M; Tagen, Michael; Bai, Feng; Stewart, Clinton F

    2011-01-01

    To study the role of drug transporters in central nervous system (CNS) penetration and cellular accumulation of erlotinib and its metabolite, OSI-420. After oral erlotinib administration to wild-type and ATP-binding cassette (ABC) transporter-knockout mice (Mdr1a/b(-/-), Abcg2(-/-), Mdr1a/b(-/-)Abcg2(-/-), and Abcc4(-/-)), plasma was collected and brain extracellular fluid (ECF) was sampled using intracerebral microdialysis. A pharmacokinetic model was fit to erlotinib and OSI-420 concentration-time data, and brain penetration (P(Brain)) was estimated by the ratio of ECF-to-unbound plasma area under concentration-time curves. Intracellular accumulation of erlotinib was assessed in cells overexpressing human ABC transporters or SLC22A solute carriers. P(Brain) in wild-type mice was 0.27 ± 0.11 and 0.07 ± 0.02 (mean ± SD) for erlotinib and OSI-420, respectively. Erlotinib and OSI-420 P(Brain) in Abcg2(-/-) and Mdr1a/b(-/-)Abcg2(-/-) mice were significantly higher than in wild-type mice. Mdr1a/b(-/-) mice showed similar brain ECF penetration as wild-type mice (0.49 ± 0.37 and 0.04 ± 0.02 for erlotinib and OSI-420, respectively). In vitro, erlotinib and OSI-420 accumulation was significantly lower in cells overexpressing breast cancer resistance protein (BCRP) than in control cells. Only OSI-420, not erlotinib, showed lower accumulation in cells overexpressing P-glycoprotein (P-gp) than in control cells. The P-gp/BCRP inhibitor elacridar increased erlotinib and OSI-420 accumulation in BCRP-overexpressing cells. Erlotinib uptake was higher in OAT3- and OCT2-transfected cells than in empty vector control cells. Abcg2 is the main efflux transporter preventing erlotinib and OSI-420 penetration in mouse brain. Erlotinib and OSI-420 are substrates for SLC22A family members OAT3 and OCT2. Our findings provide a mechanistic basis for erlotinib CNS penetration, cellular uptake, and efflux mechanisms. ©2010 AACR.

  1. 7-ketocholesteryl-9-carboxynonanoate enhances ATP binding cassette transporter A1 expression mediated by PPARγ in THP-1 macrophages.

    Science.gov (United States)

    Chi, Yan; Wang, Le; Liu, Yuanyuan; Ma, Yanhua; Wang, Renjun; Han, Xiaofei; Qiao, Hui; Lin, Jiabin; Matsuura, Eiji; Liu, Shuqian; Liu, Qingping

    2014-06-01

    ATP binding cassette transporter A1 (ABCA1) is a member of the ATP-binding cassette transporter family. It plays an essential role in mediating the efflux of excess cholesterol. It is known that peroxisome proliferator-activated receptor gamma (PPARγ) promoted ABCA1 expression. We previously found 7-ketocholesteryl-9-carboxynonanoate (oxLig-1) upregulated ABCA1 partially through CD36 mediated signals. In the present study, we intended to test if PPARγ signally is involved in the upregulation mediated by oxLig-1. First, we docked oxLig-1 and the ligand-binding domain (LBD) of PPARγ by using AutoDock 3.05 and subsequently confirmed the binding by ELISA assay. Western blotting analyses showed that oxLig-1 induces liver X receptor alpha (LXRα), PPARγ and consequently ABCA1 expression. Furthermore, oxLig-1 significantly enhanced ApoA-I-mediated cholesterol efflux. Pretreatment with an inhibitor for PPARγ (GW9662) or/and LXRα (GGPP) attenuated oxLig-1-induced ABCA1 expression. Under PPARγ knockdown by using PPARγ-shRNA, oxLig-1-induced ABCA1 expression and cholesterol efflux in THP-1 macrophages was blocked by 62% and 25% respectively. These observations suggest that oxLig-1 is a novel PPARγ agonist, promoting ApoA-I-mediated cholesterol efflux from THP-1 macrophages by increasing ABCA1 expression via induction of PPARγ. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  2. Genome-wide analysis of the ATP-binding cassette (ABC) transporter gene family in sea lamprey and Japanese lamprey.

    Science.gov (United States)

    Ren, Jianfeng; Chung-Davidson, Yu-Wen; Yeh, Chu-Yin; Scott, Camille; Brown, Titus; Li, Weiming

    2015-06-06

    Lampreys are extant representatives of the jawless vertebrate lineage that diverged from jawed vertebrates around 500 million years ago. Lamprey genomes contain information crucial for understanding the evolution of gene families in vertebrates. The ATP-binding cassette (ABC) gene family is found from prokaryotes to eukaryotes. The recent availability of two lamprey draft genomes from sea lamprey Petromyzon marinus and Japanese lamprey Lethenteron japonicum presents an opportunity to infer early evolutionary events of ABC genes in vertebrates. We conducted a genome-wide survey of the ABC gene family in two lamprey draft genomes. A total of 37 ABC transporters were identified and classified into seven subfamilies; namely seven ABCA genes, 10 ABCB genes, 10 ABCC genes, three ABCD genes, one ABCE gene, three ABCF genes, and three ABCG genes. The ABCA subfamily has expanded from three genes in sea squirts, seven and nine in lampreys and zebrafish, to 13 and 16 in human and mouse. Conversely, the multiple copies of ABCB1-, ABCG1-, and ABCG2-like genes found in sea squirts have contracted in the other species examined. ABCB2 and ABCB3 seem to be new additions in gnathostomes (not in sea squirts or lampreys), which coincides with the emergence of the gnathostome-specific adaptive immune system. All the genes in the ABCD, ABCE and ABCF subfamilies were conserved and had undergone limited duplication and loss events. In the sea lamprey transcriptomes, the ABCE and ABCF gene subfamilies were ubiquitously and highly expressed in all tissues while the members in other gene subfamilies were differentially expressed. Thirteen more lamprey ABC transporter genes were identified in this study compared with a previous study. By concatenating the same gene sequences from the two lampreys, more full length sequences were obtained, which significantly improved both the assignment of gene names and the phylogenetic trees compared with a previous analysis using partial sequences. The ABC

  3. Maltose-binding protein effectively stabilizes the partially closed conformation of the ATP-binding cassette transporter MalFGK2

    KAUST Repository

    Weng, Jingwei; Gu, Shuo; Gao, Xin; Huang, Xuhui; Wang, Wenning

    2017-01-01

    Maltose transporter MalFGK2 is a type-I importer in the ATP-binding cassette (ABC) transporter superfamily. Upon the binding of its periplasmic binding protein, MalE, the ATPase activity of MalFGK2 can be greatly enhanced. Crystal structures of the MalFGK2-MalE-maltose complex in a so-called

  4. Maltose-binding protein effectively stabilizes the partially closed conformation of the ATP-binding cassette transporter MalFGK2

    KAUST Repository

    Weng, Jingwei

    2017-02-23

    Maltose transporter MalFGK2 is a type-I importer in the ATP-binding cassette (ABC) transporter superfamily. Upon the binding of its periplasmic binding protein, MalE, the ATPase activity of MalFGK2 can be greatly enhanced. Crystal structures of the MalFGK2-MalE-maltose complex in a so-called

  5. Genome-wide identification, characterization and phylogenetic analysis of 50 catfish ATP-binding cassette (ABC) transporter genes.

    Science.gov (United States)

    Liu, Shikai; Li, Qi; Liu, Zhanjiang

    2013-01-01

    Although a large set of full-length transcripts was recently assembled in catfish, annotation of large gene families, especially those with duplications, is still a great challenge. Most often, complexities in annotation cause mis-identification and thereby much confusion in the scientific literature. As such, detailed phylogenetic analysis and/or orthology analysis are required for annotation of genes involved in gene families. The ATP-binding cassette (ABC) transporter gene superfamily is a large gene family that encodes membrane proteins that transport a diverse set of substrates across membranes, playing important roles in protecting organisms from diverse environment. In this work, we identified a set of 50 ABC transporters in catfish genome. Phylogenetic analysis allowed their identification and annotation into seven subfamilies, including 9 ABCA genes, 12 ABCB genes, 12 ABCC genes, 5 ABCD genes, 2 ABCE genes, 4 ABCF genes and 6 ABCG genes. Most ABC transporters are conserved among vertebrates, though cases of recent gene duplications and gene losses do exist. Gene duplications in catfish were found for ABCA1, ABCB3, ABCB6, ABCC5, ABCD3, ABCE1, ABCF2 and ABCG2. The whole set of catfish ABC transporters provide the essential genomic resources for future biochemical, toxicological and physiological studies of ABC drug efflux transporters. The establishment of orthologies should allow functional inferences with the information from model species, though the function of lineage-specific genes can be distinct because of specific living environment with different selection pressure.

  6. Genome-wide identification of whole ATP-binding cassette (ABC) transporters in the intertidal copepod Tigriopus japonicus.

    Science.gov (United States)

    Jeong, Chang-Bum; Kim, Bo-Mi; Lee, Jae-Seong; Rhee, Jae-Sung

    2014-08-05

    The ATP-binding cassette (ABC) transporter superfamily is one of the largest transporter gene families and is observed in all animal taxa. Although a large set of transcriptomic data was recently assembled for several species of crustaceans, identification and annotation of the large ABC transporter gene family have been very challenging. In the intertidal copepod Tigriopus japonicus, 46 putative ABC transporters were identified using in silico analysis, and their full-length cDNA sequences were characterized. Phylogenetic analysis revealed that the 46 T. japonicus ABC transporters are classified into eight subfamilies (A-H) that include all the members of all ABC subfamilies, consisting of five ABCA, five ABCB, 17 ABCC, three ABCD, one ABCE, three ABCF, seven ABCG, and five ABCH subfamilies. Of them, unique isotypic expansion of two clades of ABCC1 proteins was observed. Real-time RT-PCR-based heatmap analysis revealed that most T. japonicus ABC genes showed temporal transcriptional expression during copepod development. The overall transcriptional profile demonstrated that half of all T. japonicus ABC genes were strongly associated with at least one developmental stage. Of them, transcripts TJ-ABCH_88708 and TJ-ABCE1 were highly expressed during all developmental stages. The whole set of T. japonicus ABC genes and their phylogenetic relationships will provide a better understanding of the comparative evolution of essential gene family resources in arthropods, including the crustacean copepods.

  7. United States Geological Survey (USGS) FM cassette seismic-refraction recording system

    International Nuclear Information System (INIS)

    Murphy, J.M.

    1988-01-01

    In this two chapter report, instrumentation used to collect seismic data is described. This data acquisition system has two parts: (1) portable anolog seismic recorders and related ''hand-held-testers'' (HHT) and (2) portable digitizing units. During the anolog recording process, ground motion is sensed by a 2-Hz vertical-component seismometer. The voltage output from the seismometer is split without amplification and sent to three parallel amplifier circuit boards. Each circuit board amplifiers the seismic signal in three stages and then frequency modulates the signal. Amplification at the last two stages can be set by the user. An internal precision clock signal is also frequency modulated. The three data carrier frequencies, the clock carrier frequency, and a tape-speed compensation carrier frequency are summed and recorded on a recorded on a cassette tape. During the digitizing process, the cassette tapes are played back and the signals are demultiplexed and demodulated. An anolog-to-digital converter converts the signals to digital data which are stored on 8-inch floppy disks. 7 refs., 19 figs., 6 tabs

  8. Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.

    Directory of Open Access Journals (Sweden)

    Roya Elaine Haghighat-Khah

    Full Text Available Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests.

  9. Site-Specific Cassette Exchange Systems in the Aedes aegypti Mosquito and the Plutella xylostella Moth

    Science.gov (United States)

    Haghighat-Khah, Roya Elaine; Scaife, Sarah; Martins, Sara; St John, Oliver; Matzen, Kelly Jean; Morrison, Neil; Alphey, Luke

    2015-01-01

    Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE) mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests. PMID:25830287

  10. Downregulation of hepatic and intestinal ATP-binding-cassette transporters abcg5 and abcg8 expression associated with altered sterol fluxes in rats with streptozotocin-induced diabetes

    NARCIS (Netherlands)

    Bloks, VW; Bakker-van Waarde, WW; Verkade, HJ; Kema, IP; Havinga, R; Wolters, H; Schaap, FG; Sauer, PJJ; Vink, E; Groen, AK; Kuipers, F

    ABSTRACT: P234 Downregulation of Hepatic and Intestinal ATP-Binding-Cassette Transporters Abcg5 and Abcg8 Expression Associated with Altered Sterol Fluxes in Rats with Streptozotocin-Induced Diabetes Vincent W. Bloks, Willie W. Bakker-van Waarde, Henkjan J. Verkade, Ido P. Kema, Rick Havinga, Henk

  11. Molecular cloning and expression profile of an ATP-binding cassette (ABC) transporter gene from the hemipteran insect Nilaparvata lugens.

    Science.gov (United States)

    Zha, W J; Li, S H; Zhou, L; Chen, Z J; Liu, K; Yang, G C; Hu, G; He, G C; You, A Q

    2015-03-30

    The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. In insects, ABC transporters have important functions in the transport of molecules, and are also involved in insecticide resistance, metabolism, and development. In this study, the Nilaparvata lugens Stal (Hemiptera: Delphacidae) ABCG (NlABCG) gene was identified and characterized. The complete mRNA sequence of NlABCG was 2608-bp long, with an open reading frame of 2064 bp encoding a protein comprised of 687 amino acids. The conserved regions include three N-glycosylation and 34 phosphorylation sites, as well as seven transmembrane domains. The amino acid identity with the closely related species Acyrthosiphon pisum was 42.8%. Developmental expression analysis using quantitative real-time reverse transcriptase PCR suggested that the NlABCG transcript was expressed at all developmental stages of N. lugens. The lowest expression of NlABCG was in the 1st instar, and levels increased with larval growth. The transcript profiles of NlABCG were analyzed in various tissues from a 5th instar nymph, and the highest expression was observed in the midgut. These results suggest that the sequence, characteristics, and expression of NlABCG are highly conserved, and basic information is provided for its functional analysis.

  12. Hop resistance in the beer spoilage bacterium Lactobacillus brevis is mediated by the ATP-binding cassette multidrug transporter HorA.

    Science.gov (United States)

    Sakamoto, K; Margolles, A; van Veen, H W; Konings, W N

    2001-09-01

    Lactobacillus brevis is a major contaminant of spoiled beer. The organism can grow in beer in spite of the presence of antibacterial hop compounds that give the beer a bitter taste. The hop resistance in L. brevis is, at least in part, dependent on the expression of the horA gene. The deduced amino acid sequence of HorA is 53% identical to that of LmrA, an ATP-binding cassette multidrug transporter in Lactococcus lactis. To study the role of HorA in hop resistance, HorA was functionally expressed in L. lactis as a hexa-histidine-tagged protein using the nisin-controlled gene expression system. HorA expression increased the resistance of L. lactis to hop compounds and cytotoxic drugs. Drug transport studies with L. lactis cells and membrane vesicles and with proteoliposomes containing purified HorA protein identified HorA as a new member of the ABC family of multidrug transporters.

  13. Characterization and expression profiling of ATP-binding cassette transporter genes in the diamondback moth, Plutella xylostella (L.).

    Science.gov (United States)

    Qi, Weiping; Ma, Xiaoli; He, Weiyi; Chen, Wei; Zou, Mingmin; Gurr, Geoff M; Vasseur, Liette; You, Minsheng

    2016-09-27

    ATP-binding cassette (ABC) transporters are one of the major transmembrane protein families found in all organisms and play important roles in transporting a variety of compounds across intra and extra cellular membranes. In some species, ABC transporters may be involved in the detoxification of substances such as insecticides. The diamondback moth, Plutella xylostella (L.), a destructive pest of cruciferous crops worldwide, is an important species to study as it is resistant to many types of insecticides as well as biological control Bacillus thuringiensis toxins. A total of 82 ABC genes were identified from our published P. xylostella genome, and grouped into eight subfamilies (ABCA-H) based on phylogenetic analysis. Genes of subfamilies ABCA, ABCC and ABCH were found to be expanded in P. xylostella compared with those in Bombyx mori, Manduca sexta, Heliconius melpomene, Danaus plexippus, Drosophila melanogaster, Tetranychus urticae and Homo sapiens. Phylogenetic analysis indicated that many of the ABC transporters in P. xylostella are orthologous to the well-studied ABC transporter genes in the seven other species. Transcriptome- and qRT-PCR-based analysis elucidated physiological effects of ABC gene expressions of P. xylostella which were developmental stage- and tissue-specific as well as being affected by whether or not the insects were from an insecticide-resistant strain. Two ABCC and one ABCA genes were preferentially expressed in midgut of the 4th-instar larvae of a susceptible strain (Fuzhou-S) suggesting their potential roles in metabolizing plant defensive chemicals. Most of the highly expressed genes in insecticide-resistant strains were also predominantly expressed in the tissues of Malpighian tubules and midgut. This is the most comprehensive study on identification, characterization and expression profiling of ABC transporter genes in P. xylostella to date. The diversified features and expression patterns of this gene family may be associated with

  14. Pharmacogenetic Aspects of the Interaction of AT1 Receptor Antagonists With ATP-Binding Cassette Transporter ABCG2

    Directory of Open Access Journals (Sweden)

    Anne Ripperger

    2018-05-01

    Full Text Available The ATP-binding cassette transporter ABCG2 (BCRP and MXR is involved in the absorption, distribution, and elimination of numerous drugs. Thus, drugs that are able to reduce the activity of ABCG2, e.g., antihypertensive AT1 receptor antagonists (ARBs, may cause drug-drug interactions and compromise drug safety and efficacy. In addition, genetic variability within the ABCG2 gene may influence the ability of the transporter to interact with ARBs. Thus, the aim of this study was to characterize the ARB-ABCG2 interaction in the light of naturally occurring variations (F489L, R482G or amino acid substitutions with in silico-predicted relevance for the ARB-ABCG2 interaction (Y469A; M483F; Y570A. For this purpose, ABCG2 variants were expressed in HEK293 cells and the impact of ARBs on ABCG2 activity was studied in vitro using the pheophorbide A (PhA efflux assay. First, we demonstrated that both the F489L and the Y469A substitution, respectively, reduced ABCG2 protein levels in these cells. Moreover, both substitutions enhanced the inhibitory effect of candesartan cilexetil, irbesartan, losartan, and telmisartan on ABCG2-mediated PhA efflux, whereas the R482G substitution blunted the inhibitory effect of candesartan cilexetil and telmisartan in this regard. In contrast, the ARB-ABCG2 interaction was not altered in cells expressing either the M483F or the Y570A variant, respectively. In conclusion, our data indicate that the third transmembrane helix and adjacent regions of ABCG2 may be of major importance for the interaction of ARBs with the ABC transporter. Moreover, we conclude from our data that individuals carrying the F489L polymorphism may be at increased risk of developing ABCG2-related drug-drug interactions in multi-drug regimens involving ARBs.

  15. Molecular cloning and functional characterization of an ATP-binding cassette transporter OtrC from Streptomyces rimosus

    Directory of Open Access Journals (Sweden)

    Yu Lan

    2012-08-01

    Full Text Available Abstract Background The otrC gene of Streptomyces rimosus was previously annotated as an oxytetracycline (OTC resistance protein. However, the amino acid sequence analysis of OtrC shows that it is a putative ATP-binding cassette (ABC transporter with multidrug resistance function. To our knowledge, none of the ABC transporters in S. rimosus have yet been characterized. In this study, we aimed to characterize the multidrug exporter function of OtrC and evaluate its relevancy to OTC production. Results In order to investigate OtrC’s function, otrC is cloned and expressed in E. coli The exporter function of OtrC was identified by ATPase activity determination and ethidium bromide efflux assays. Also, the susceptibilities of OtrC-overexpressing cells to several structurally unrelated drugs were compared with those of OtrC-non-expressing cells by minimal inhibitory concentration (MIC assays, indicating that OtrC functions as a drug exporter with a broad range of drug specificities. The OTC production was enhanced by 1.6-fold in M4018 (P = 0.000877 and 1.4-fold in SR16 (P = 0.00973 duplication mutants, while it decreased to 80% in disruption mutants (P = 0.0182 and 0.0124 in M4018 and SR16, respectively. Conclusions The results suggest that OtrC is an ABC transporter with multidrug resistance function, and plays an important role in self-protection by drug efflux mechanisms. This is the first report of such a protein in S. rimosus, and otrC could be a valuable target for genetic manipulation to improve the production of industrial antibiotics.

  16. Association of ATP-Binding Cassette Transporter A1 Gene Polymorphisms in Type 2 Diabetes Mellitus among Malaysians

    Directory of Open Access Journals (Sweden)

    Polin Haghvirdizadeh

    2015-01-01

    Full Text Available Background. Type 2 diabetes mellitus (T2DM is a complex polygenic disorder characterized by impaired insulin resistance, insulin secretion, and dysregulation of lipid and protein metabolism with environmental and genetic factors. ATP-binding cassette transporter A1 (ABCA1 gene polymorphisms are reported as the one of the genetic risk factors for T2DM in various populations with conflicting results. This study was conducted based on PCR-HRM to determine the frequency of ABCA1 gene by rs2230806 (R219K, rs1800977 (C69T, and rs9282541 (R230C polymorphisms Malaysian subjects. Methods. A total of 164 T2DM and 165 controls were recruited and their genotypes for ABCA1 gene polymorphisms were determined based on the real time high resolution melting analysis. Results. There was a significant difference between the subjects in terms of age, BMI, FPG, HbA1c, HDL, LDL, and TG P<0.05. There was a significant association between HOM of R219K P=0.005, among Malaysian subjects; moreover, allele frequency revealed the significant difference in A allele of R219K P=0.003. But, there was no significant difference in genotypic and allelic frequencies of C69T and R230C polymorphism. Conclusion. R219K polymorphism of ABCA1 gene can be considered as a genetic risk factor for T2DM subjects among Malaysians.

  17. Genome-wide analysis of the ATP-binding cassette (ABC) transporter gene family in the silkworm, Bombyx mori.

    Science.gov (United States)

    Xie, Xiaodong; Cheng, Tingcai; Wang, Genhong; Duan, Jun; Niu, Weihuan; Xia, Qingyou

    2012-07-01

    The ATP-binding cassette (ABC) superfamily is a larger protein family with diverse physiological functions in all kingdoms of life. We identified 53 ABC transporters in the silkworm genome, and classified them into eight subfamilies (A-H). Comparative genome analysis revealed that the silkworm has an expanded ABCC subfamily with more members than Drosophila melanogaster, Caenorhabditis elegans, or Homo sapiens. Phylogenetic analysis showed that the ABCE and ABCF genes were highly conserved in the silkworm, indicating possible involvement in fundamental biological processes. Five multidrug resistance-related genes in the ABCB subfamily and two multidrug resistance-associated-related genes in the ABCC subfamily indicated involvement in biochemical defense. Genetic variation analysis revealed four ABC genes that might be evolving under positive selection. Moreover, the silkworm ABCC4 gene might be important for silkworm domestication. Microarray analysis showed that the silkworm ABC genes had distinct expression patterns in different tissues on day 3 of the fifth instar. These results might provide new insights for further functional studies on the ABC genes in the silkworm genome.

  18. Disruption of lolCDE, Encoding an ATP-Binding Cassette Transporter, Is Lethal for Escherichia coli and Prevents Release of Lipoproteins from the Inner Membrane

    OpenAIRE

    Narita, Shin-ichiro; Tanaka, Kimie; Matsuyama, Shin-ichi; Tokuda, Hajime

    2002-01-01

    ATP-binding cassette transporter LolCDE was previously identified, by using reconstituted proteoliposomes, as an apparatus catalyzing the release of outer membrane-specific lipoproteins from the inner membrane of Escherichia coli. Mutations resulting in defective LolD were previously shown to be lethal for E. coli. The amino acid sequences of LolC and LolE are similar to each other, but the necessity of both proteins for lipoprotein release has not been proved. Moreover, previous reconstituti...

  19. Concept design of the DEMO divertor cassette-to-vacuum vessel locking system adopting a systems engineering approach

    International Nuclear Information System (INIS)

    Di Gironimo, G.; Carfora, D.; Esposito, G.; Lanzotti, A.; Marzullo, D.; Siuko, M.

    2015-01-01

    Highlights: • An iterative and incremental design process for cassette-to-VV locking system of DEMO divertor is presented. • Three different concepts have been developed with a systematic design approach. • The final concept has been selected with Fuzzy-Analytic Hierarchy Process in virtual reality. - Abstract: This paper deals with pre-concept studies of DEMO divertor cassette-to-vacuum vessel locking system under the work program WP13-DAS-07-T06: Divertor Remote Maintenance System pre-concept study. An iterative design process, consistent with Systems Engineering guidelines and named Iterative and Participative Axiomatic Design Process (IPADeP), is used in this paper to propose new innovative solutions for divertor locking system, which can overcome the difficulties in applying the ITER principles to DEMO. The solutions conceived have been analysed from the structural point of view using the software Ansys and, eventually, evaluated using the methodology known as Fuzzy-Analytic Hierarchy Process. Due to the lack and the uncertainty of the requirements in this early conceptual design stage, the aim is to cover a first iteration of an iterative and incremental process to propose an innovative design concept to be developed in more details as the information will be completed

  20. Concept design of the DEMO divertor cassette-to-vacuum vessel locking system adopting a systems engineering approach

    Energy Technology Data Exchange (ETDEWEB)

    Di Gironimo, G., E-mail: giuseppe.digironimo@unina.it [Università degli Studi di Napoli “Federico II”, Dipartimento di Ingegneria Industriale, Piazzale Tecchio 80, 80135 Napoli (Italy); Carfora, D. [Tampere University of Technology, Korkeakoulunkatu 6, 33720 Tampere (Finland); VTT Technical Research Centre of Finland, Tekniikankatu 1, PO Box 1300, FI-33101 Tampere (Finland); Università degli Studi di Napoli “Federico II”, Dipartimento di Ingegneria Industriale, Piazzale Tecchio 80, 80135 Napoli (Italy); Esposito, G.; Lanzotti, A.; Marzullo, D. [Università degli Studi di Napoli “Federico II”, Dipartimento di Ingegneria Industriale, Piazzale Tecchio 80, 80135 Napoli (Italy); Siuko, M. [VTT Technical Research Centre of Finland, Tekniikankatu 1, PO Box 1300, FI-33101 Tampere (Finland)

    2015-05-15

    Highlights: • An iterative and incremental design process for cassette-to-VV locking system of DEMO divertor is presented. • Three different concepts have been developed with a systematic design approach. • The final concept has been selected with Fuzzy-Analytic Hierarchy Process in virtual reality. - Abstract: This paper deals with pre-concept studies of DEMO divertor cassette-to-vacuum vessel locking system under the work program WP13-DAS-07-T06: Divertor Remote Maintenance System pre-concept study. An iterative design process, consistent with Systems Engineering guidelines and named Iterative and Participative Axiomatic Design Process (IPADeP), is used in this paper to propose new innovative solutions for divertor locking system, which can overcome the difficulties in applying the ITER principles to DEMO. The solutions conceived have been analysed from the structural point of view using the software Ansys and, eventually, evaluated using the methodology known as Fuzzy-Analytic Hierarchy Process. Due to the lack and the uncertainty of the requirements in this early conceptual design stage, the aim is to cover a first iteration of an iterative and incremental process to propose an innovative design concept to be developed in more details as the information will be completed.

  1. Evaluation of the role of ATP-binding cassette transporters as a defence mechanism against temephos in populations of Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Estelita Pereira Lima

    2014-11-01

    Full Text Available The role of ATP-binding cassette (ABC transporters in the efflux of the insecticide, temephos, was assessed in the larvae of Aedes aegypti. Bioassays were conducted using mosquito populations that were either susceptible or resistant to temephos by exposure to insecticide alone or in combination with sublethal doses of the ABC transporter inhibitor, verapamil (30, 35 and 40 μM. The best result in the series was obtained with the addition of verapamil (40 μM, which led to a 2x increase in the toxicity of temephos, suggesting that ABC transporters may be partially involved in conferring resistance to the populations evaluated.

  2. Transport system

    NARCIS (Netherlands)

    Drenth, K.F.

    1999-01-01

    The transport system comprises at least one road surface (2) and at least one vehicle (4) on wheels (6). The road surface (2) has a substantially bowl-shaped cross section and the vehicle (4) is designed so that the wheels (6) run directly on the road surface (2) while the road surface (2) acts as a

  3. AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene.

    Science.gov (United States)

    Lu, Y P; Li, Z S; Rea, P A

    1997-07-22

    Because plants produce cytotoxic compounds to which they, themselves, are susceptible and are exposed to exogenous toxins (microbial products, allelochemicals, and agrochemicals), cell survival is contingent on mechanisms for detoxifying these agents. One detoxification mechanism is the glutathione S-transferase-catalyzed glutathionation of the toxin, or an activated derivative, and transport of the conjugate out of the cytosol. We show here that a transporter responsible for the removal of glutathione S-conjugates from the cytosol, a specific Mg2+-ATPase, is encoded by the AtMRP1 gene of Arabidopsis thaliana. The sequence of AtMRP1 and the transport capabilities of membranes prepared from yeast cells transformed with plasmid-borne AtMRP1 demonstrate that this gene encodes an ATP-binding cassette transporter competent in the transport of glutathione S-conjugates of xenobiotics and endogenous substances, including herbicides and anthocyanins.

  4. Fabrication of divertor cassette for ITER

    International Nuclear Information System (INIS)

    Sanguinetti, G.P.

    2008-01-01

    The Divertor is the component located on the bottom of the ITER vacuum vessel, whose main function is to adsorb the high thermal flux generated by the plasma whilst keeping the plasma impurity at a reasonable low level. The divertor consist of 54 units, each comprising outer components, facing the plasma and a component supporting the plasma facing components (PFC) and providing coolant distribution to them (divertor cassette). The divertor cassette is a box structure, butt welded and machined, made from plates and forgins of austenitic stainless steels. The cassette fabrication, which is in detail described, includes manufacturing of the attachments of the PFC to the cassette, the coolant distribution channels, and the cassette to vacuum vessel locking system. The divertor cassette is a pressure component (the cooling water runs at 40 bar) and therefore divertor cassette design, fabrication and service shall comply with the European PED and the applicable French law for the ITER. (orig.)

  5. NpPDR1, a Pleiotropic Drug Resistance-Type ATP-Binding Cassette Transporter from Nicotiana plumbaginifolia, Plays a Major Role in Plant Pathogen Defense1

    Science.gov (United States)

    Stukkens, Yvan; Bultreys, Alain; Grec, Sébastien; Trombik, Tomasz; Vanham, Delphine; Boutry, Marc

    2005-01-01

    Nicotiana plumbaginifolia NpPDR1, a plasma membrane pleiotropic drug resistance-type ATP-binding cassette transporter formerly named NpABC1, has been suggested to transport the diterpene sclareol, an antifungal compound. However, direct evidence for a role of pleiotropic drug resistance transporters in the plant defense is still lacking. In situ immunolocalization and histochemical analysis using the gusA reporter gene showed that NpPDR1 was constitutively expressed in the whole root, in the leaf glandular trichomes, and in the flower petals. However, NpPDR1 expression was induced in the whole leaf following infection with the fungus Botrytis cinerea, and the bacteria Pseudomonas syringae pv tabaci, Pseudomonas fluorescens, and Pseudomonas marginalis pv marginalis, which do not induce a hypersensitive response in N. plumbaginifolia, whereas a weaker response was observed using P. syringae pv syringae, which does induce a hypersensitive response. Induced NpPDR1 expression was more associated with the jasmonic acid than the salicylic acid signaling pathway. These data suggest that NpPDR1 is involved in both constitutive and jasmonic acid-dependent induced defense. Transgenic plants in which NpPDR1 expression was prevented by RNA interference showed increased sensitivity to sclareol and reduced resistance to B. cinerea. These data show that NpPDR1 is involved in pathogen resistance and thus demonstrate a new role for the ATP-binding cassette transporter family. PMID:16126865

  6. NpPDR1, a pleiotropic drug resistance-type ATP-binding cassette transporter from Nicotiana plumbaginifolia, plays a major role in plant pathogen defense.

    Science.gov (United States)

    Stukkens, Yvan; Bultreys, Alain; Grec, Sébastien; Trombik, Tomasz; Vanham, Delphine; Boutry, Marc

    2005-09-01

    Nicotiana plumbaginifolia NpPDR1, a plasma membrane pleiotropic drug resistance-type ATP-binding cassette transporter formerly named NpABC1, has been suggested to transport the diterpene sclareol, an antifungal compound. However, direct evidence for a role of pleiotropic drug resistance transporters in the plant defense is still lacking. In situ immunolocalization and histochemical analysis using the gusA reporter gene showed that NpPDR1 was constitutively expressed in the whole root, in the leaf glandular trichomes, and in the flower petals. However, NpPDR1 expression was induced in the whole leaf following infection with the fungus Botrytis cinerea, and the bacteria Pseudomonas syringae pv tabaci, Pseudomonas fluorescens, and Pseudomonas marginalis pv marginalis, which do not induce a hypersensitive response in N. plumbaginifolia, whereas a weaker response was observed using P. syringae pv syringae, which does induce a hypersensitive response. Induced NpPDR1 expression was more associated with the jasmonic acid than the salicylic acid signaling pathway. These data suggest that NpPDR1 is involved in both constitutive and jasmonic acid-dependent induced defense. Transgenic plants in which NpPDR1 expression was prevented by RNA interference showed increased sensitivity to sclareol and reduced resistance to B. cinerea. These data show that NpPDR1 is involved in pathogen resistance and thus demonstrate a new role for the ATP-binding cassette transporter family.

  7. Genetic Analysis of the Mode of Interplay between an ATPase Subunit and Membrane Subunits of the Lipoprotein-Releasing ATP-Binding Cassette Transporter LolCDE†

    OpenAIRE

    Ito, Yasuko; Matsuzawa, Hitomi; Matsuyama, Shin-ichi; Narita, Shin-ichiro; Tokuda, Hajime

    2006-01-01

    The LolCDE complex, an ATP-binding cassette (ABC) transporter, releases lipoproteins from the inner membrane, thereby initiating lipoprotein sorting to the outer membrane of Escherichia coli. The LolCDE complex is composed of two copies of an ATPase subunit, LolD, and one copy each of integral membrane subunits LolC and LolE. LolD hydrolyzes ATP on the cytoplasmic side of the inner membrane, while LolC and/or LolE recognize and release lipoproteins anchored to the periplasmic leaflet of the i...

  8. Hop Resistance in the Beer Spoilage Bacterium Lactobacillus brevis Is Mediated by the ATP-Binding Cassette Multidrug Transporter HorA

    OpenAIRE

    Sakamoto, Kanta; Margolles, Abelardo; van Veen, Hendrik W.; Konings, Wil N.

    2001-01-01

    Lactobacillus brevis is a major contaminant of spoiled beer. The organism can grow in beer in spite of the presence of antibacterial hop compounds that give the beer a bitter taste. The hop resistance in L. brevis is, at least in part, dependent on the expression of the horA gene. The deduced amino acid sequence of HorA is 53% identical to that of LmrA, an ATP-binding cassette multidrug transporter in Lactococcus lactis. To study the role of HorA in hop resistance, HorA was functionally expre...

  9. Overexpression of the ATP-binding cassette half-transporter, ABCG2 (Mxr/BCrp/ABCP1), in flavopiridol-resistant human breast cancer cells

    DEFF Research Database (Denmark)

    Robey, R W; Medina-Pérez, W Y; Nishiyama, K

    2001-01-01

    We sought to characterize the interactions of flavopiridol with members of the ATP-binding cassette (ABC) transporter family. Cells overexpressing multidrug resistance-1 (MDR-1) and multidrug resistance-associated protein (MRP) did not exhibit appreciable flavopiridol resistance, whereas cell lines...... overexpressing the ABC half-transporter, ABCG2 (MXR/BCRP/ABCP1), were found to be resistant to flavopiridol. Flavopiridol at a concentration of 10 microM was able to prevent MRP-mediated calcein efflux, whereas Pgp-mediated transport of rhodamine 123 was unaffected at flavopiridol concentrations of up to 100...... analysis revealed overexpression of the ABCG2 gene. Western blot confirmed overexpression of ABCG2; neither P-glycoprotein nor MRP overexpression was detected. These results suggest that ABCG2 plays a role in resistance to flavopiridol....

  10. Effect of praziquantel on the differential expression of mouse hepatic genes and parasite ATP binding cassette transporter gene family members during Schistosoma mansoni infection.

    Directory of Open Access Journals (Sweden)

    Melissa C Sanchez

    2017-06-01

    Full Text Available Schistosomiasis is a chronic parasitic disease caused by sexually dimorphic blood flukes of the genus Schistosoma. Praziquantel (PZQ is the only drug widely available to treat the disease but does not kill juvenile parasites. Here we report the use of next generation sequencing to study the transcriptional effect of PZQ on murine hepatic inflammatory, immune and fibrotic responses to Schistosoma mansoni worms and eggs. An initial T helper cell 1 (Th1 response is induced against schistosomes in mice treated with drug vehicle (Vh around the time egg laying begins, followed by a T helper cell 2 (Th2 response and the induction of genes whose action leads to granuloma formation and fibrosis. When PZQ is administered at this time, there is a significant reduction in egg burden yet the hepatic Th1, Th2 and fibrotic responses are still observed in the absence of granuloma formation suggesting some degree of gene regulation may be induced by antigens released from the dying adult worms. Quantitative real-time PCR was used to examine the relative expression of 16 juvenile and adult S. mansoni genes during infection and their response to Vh and PZQ treatment in vivo. While the response of stress genes in adult parasites suggests the worms were alive immediately following exposure to PZQ, they were unable to induce transcription of any of the 9 genes encoding ATP-binding cassette (ABC transporters tested. In contrast, juvenile schistosomes were able to significantly induce the activities of ABCB, C and G family members, underscoring the possibility that these efflux systems play a major role in drug resistance.

  11. Divertor cassette movers prototypes for ITER

    International Nuclear Information System (INIS)

    Bogusch, E.; Batz, R.; Bieber, O.; Gottfried, R.; Cerdan, G.

    1998-01-01

    Following competitive tendering, in October 1996 Siemens was contracted by the European Commission to design and supply an assembly of four Divertor Cassette Movers Prototypes including the control and command systems for the movers proper. The assembly consisting of one Cassette Toroidal Mover (CTM), one Radial Mover Tractor (TRC), one Second Cassette Carrier (SCC), and one Radial Cassette Carrier (RCC) represents key components of the Divertor Test Platform at Brasimone, one of the seven large R+D projects for ITER. By detailed design, high-precision manufacturing and testing of these devices, Siemens contributed to the verification of an important task within the European R and D program towards ITER construction. Replacement of the divertor cassettes is a scheduled maintenance operation throughout the life of ITER. The successful fabrication and testing of the Divertor Cassette Movers Prototypes is all important milestone to verify this delicate operation. (authors)

  12. Inventory and analysis of ATP-binding cassette (ABC) systems in Brugia malayi.

    Science.gov (United States)

    Ardelli, B F; Stitt, L E; Tompkins, J B

    2010-07-01

    ABC systems are one of the largest described protein superfamilies. These systems have a domain organization that may contain 1 or more transmembrane domains (ABC_TM1F) and 1 or 2 ATP-binding domains (ABC_2). The functions (e.g., import, export and DNA repair) of these proteins distinguish the 3 classes of ABC systems. Mining and PCR-based cloning were used to identify 33 putative ABC systems from the Brugia malayi genome. There were 31 class 2 genes, commonly called ABC transporters, and 2 class 3 genes. The ABC transporters were divided into subfamilies. Three belonged to subfamily A, 16 to subfamily B, 5 to subfamily C, 1 to subfamily E and 3 to subfamilies F and G, respectively. None were placed in subfamilies D and H. Similar to other ABC systems, the ABC_2 domain of B. malayi genes was conserved and contained the Walker A and B motifs, the signature sequence/linker region and the switch region with the conserved histidine. The ABC_TM1F domain was less conserved. The relative abundance of ABC systems was quantified using real-time reverse transcription PCR and was significantly higher in female adults of B. malayi than in males and microfilaria, particularly those in subfamilies B and C, which are associated with drug resistance.

  13. The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity.

    Science.gov (United States)

    Marek, Magdalena; Milles, Sigrid; Schreiber, Gabriele; Daleke, David L; Dittmar, Gunnar; Herrmann, Andreas; Müller, Peter; Pomorski, Thomas Günther

    2011-06-17

    The ATP binding cassette (ABC) transporter Aus1 is expressed under anaerobic growth conditions at the plasma membrane of the yeast Saccharomyces cerevisiae and is required for sterol uptake. These observations suggest that Aus1 promotes the translocation of sterols across membranes, but the precise transport mechanism has yet to be identified. In this study, an extraction and purification procedure was developed to characterize the Aus1 transporter. The detergent-solubilized protein was able to bind and hydrolyze ATP. Mutagenesis of the conserved lysine to methionine in the Walker A motif abolished ATP hydrolysis. Likewise, ATP hydrolysis was inhibited by classical inhibitors of ABC transporters. Upon reconstitution into proteoliposomes, the ATPase activity of Aus1 was specifically stimulated by phosphatidylserine (PS) in a stereoselective manner. We also found that Aus1-dependent sterol uptake, but not Aus1 expression and trafficking to the plasma membrane, was affected by changes in cellular PS levels. These results suggest a direct interaction between Aus1 and PS that is critical for the activity of the transporter.

  14. Reduction of radiation dose by using digital luminescence radiography compared to conventional screen film system with grid cassette

    International Nuclear Information System (INIS)

    Heyne, J.P.; Merbold, H.; Neumann, R.; Freesmeyer, M.; Jonetz-Mentzel, L.; Kaiser, W.A.; Sehner, J.

    1999-01-01

    Purpose: How much can the radiation dose be reduced for skull radiography by using digital luminescence radiography (DLR) compared to a conventional screen film system with a grid cassette? Methods and Materials: A skull phantom (3M) was X-rayed in anterior-posterior orientation using both a conventional screen film system with grid cassette and DLR (ADC-70, Agfa). The tube current time product (mAs) was diminished gradually while keeping the voltage constant. The surface entrance dose was measured by a sensor of Dosimax (Wellhoefer). Five investigators evaluated the images by characteristic and critical features, spatial resolution and contrast. Results: The surface entrance dose at 73 kV/22 mAs was 0,432 mGy in conventional screen film system and 0,435 mGy in DLR. The images could be evaluated very well down to an average dose of 71% (0,308 mGy; SD 0,050); sufficient images were obtained down to an average dose of 31% (0,136 mGy; SD 0,065). The resolution of the line pairs were reduced down to a 2 levels depending on the investigator. Contrast was assessed as being very good to sufficient. The acceptance of the postprocessed images (MUSICA-software) was individually different and resultde in an improvement of the assessment of bone structures an contrast in higher dose ranges only. Conclusion: For the sufficient assessment of a possible fracture/of paranasal sinuses/of measurement the skull the dose can be reduced to at least 56% (31%; SD 14,9%)/40% (27%; SD 9,3%)/18% (14%; SD 4,4%). Digital radiography allows question-referred exposure parameters with clearly reduced dose, so e.g. for fracture exclusion 73 kV/12,5 mAs and to skull measurement 73 kV/4 mAs. (orig.) [de

  15. An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice.

    Science.gov (United States)

    Chen, Guoxiong; Komatsuda, Takao; Ma, Jian Feng; Nawrath, Christiane; Pourkheirandish, Mohammad; Tagiri, Akemi; Hu, Yin-Gang; Sameri, Mohammad; Li, Xinrong; Zhao, Xin; Liu, Yubing; Li, Chao; Ma, Xiaoying; Wang, Aidong; Nair, Sudha; Wang, Ning; Miyao, Akio; Sakuma, Shun; Yamaji, Naoki; Zheng, Xiuting; Nevo, Eviatar

    2011-07-26

    Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.

  16. Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5.

    Science.gov (United States)

    Matsuda, Shuichi; Takano, Sho; Sato, Moeko; Furukawa, Kaoru; Nagasawa, Hidetaka; Yoshikawa, Shoko; Kasuga, Jun; Tokuji, Yoshihiko; Yazaki, Kazufumi; Nakazono, Mikio; Takamure, Itsuro; Kato, Kiyoaki

    2016-03-07

    Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette (ABC) subfamily G protein, RCN1/OsABCG5, is involved in stomatal closure mediated by phytohormone abscisic acid (ABA) accumulation in guard cells. We found that the GFP-RCN1/OsABCG5-fusion protein was localized at the plasma membrane in guard cells. The percentage of guard cell pairs containing both ABA and GFP-RCN1/OsABCG5 increased after exogenous ABA treatment, whereas they were co-localized in guard cell pairs regardless of whether exogenous ABA was applied. ABA application resulted in a smaller increase in the percentage of guard cell pairs containing ABA in rcn1 mutant (A684P) and RCN1-RNAi than in wild-type plants. Furthermore, polyethylene glycol (drought stress)-inducible ABA accumulation in guard cells did not occur in rcn1 mutants. Stomata closure mediated by exogenous ABA application was strongly reduced in rcn1 mutants. Finally, rcn1 mutant plants had more rapid water loss from detached leaves than the wild-type plants. These results indicate that in response to drought stress, RCN1/OsABCG5 is involved in accumulation of ABA in guard cells, which is indispensable for stomatal closure. Copyright © 2016 The Author. Published by Elsevier Inc. All rights reserved.

  17. Universal modular dosimetric cassette

    International Nuclear Information System (INIS)

    Plichta, J.; Singer, J.; Trousil, J.; Pohnetal, F.; Kreisinger, F.; Feik, K.; Vaclavek, Z.; Jansky, B.

    1987-01-01

    The personnel dosemeter cassette is made of plastic and consists of front and rear parts joined with projections and a lock. The inner part is provided with recesses for securing different filters in position by gluing. A connecting part allows to firmly and easily lock more cassettes for the purposes of simultaneous measurement using different dosemeters. (M.D.). 6 figs

  18. The naphthoquinones, vitamin K3 and its structural analogue plumbagin, are substrates of the multidrug resistance linked ATP binding cassette drug transporter ABCG2.

    Science.gov (United States)

    Shukla, Suneet; Wu, Chung-Pu; Nandigama, Krishnamachary; Ambudkar, Suresh V

    2007-12-01

    Vitamin K3 (menadione; 2-methyl-1,4-naphthoquinone) is a structural precursor of vitamins K1 and K2, which are essential for blood clotting. The naturally occurring structural analogue of this vitamin, plumbagin (5-hydroxy-menadione), is known to modulate cellular proliferation, apoptosis, carcinogenesis, and radioresistance. We here report that both vitamin K3 and plumbagin are substrates of the multidrug resistance-linked ATP binding cassette drug transporter, ABCG2. Vitamin K3 and plumbagin specifically inhibited the ABCG2-mediated efflux of mitoxantrone but did not have any effect on the ABCB1-mediated efflux of rhodamine 123. This inhibition of ABCG2 function was due to their interaction at the substrate-binding site(s). Vitamin K3 and plumbagin inhibited the binding of [(125)I]iodoarylazidoprazosin, a substrate of ABCG2, to this transporter in a concentration-dependent manner with IC(50) values of 7.3 and 22.6 micromol/L, respectively, but had no effect on the binding of the photoaffinity analogue to ABCB1. Both compounds stimulated ABCG2-mediated ATP hydrolysis and also inhibited the mitoxantrone-stimulated ATPase activity of the ABCG2 transporter, but did not have any significant effect on the ATPase activity of ABCB1. In a cytotoxicity assay, ABCG2-expressing HEK cells were 2.8- and 2.3-fold resistant to plumbagin and vitamin K3, respectively, compared with the control cells, suggesting that they are substrates of this transporter. Collectively, these data show for the first time that vitamin K3 is a substrate of the ABCG2 transporter. Thus, ABCG2 may have a role in the regulation of vitamin K3 levels in the body. In addition, vitamin K3 and its structural derivative, plumbagin, could potentially be used to modulate ABCG2 function.

  19. ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function

    Directory of Open Access Journals (Sweden)

    Wen-Jung Lu

    2018-03-01

    Full Text Available Vibrio cholerae ATP-binding cassette transporter VcaM (V. cholerae ABC multidrug resistance pump has previously been shown to confer resistance to a variety of medically important drugs. In this study, we set to analyse its properties both in vitro in detergent-solubilised state and in vivo to differentiate its dependency on auxiliary proteins for its function. We report the first detailed kinetic parameters of purified VcaM and the rate of phosphate (Pi production. To determine the possible functional dependencies of VcaM on the tripartite efflux pumps we then utilized different E. coli strains lacking the principal secondary transporter AcrB (Acriflavine resistance protein, as well as cells lacking the outer membrane factor (OMF TolC (Tolerance to colicins. Consistent with the ATPase function of VcaM we found it to be susceptible to sodium orthovanadate (NaOV, however, we also found a clear dependency of VcaM function on TolC. Inhibitors targeting secondary active transporters had no effects on either VcaM-conferred resistance or Hoechst 33342 accumulation, suggesting that VcaM might be capable of engaging with the TolC-channel without periplasmic mediation by additional transporters. Our findings are indicative of VcaM being capable of a one-step substrate translocation from cytosol to extracellular space utilising the TolC-channel, making it the only multidrug ABC-transporter outside of the MacB-family with demonstrable TolC-dependency.

  20. ATP-binding cassette transporters P-glycoprotein and breast cancer related protein are reduced in capillary cerebral amyloid angiopathy

    NARCIS (Netherlands)

    Carrano, A.; Snkhchyan, H.; Kooij, G.; van der Pol, S.; van Horssen, J.; Veerhuis, R.; Hoozemans, J.J.M.; Rozemuller, A.J.M.; de Vries, H.E.

    2014-01-01

    Alzheimer's disease (AD) is the most common form of dementia and marked by deposition of amyloid-β (Aβ) within the brain. Alterations of Aβ transporters at the neurovasculature may play a role in the disease process. We investigated the expression of ABC transporters P-glycoprotein (P-gp) and breast

  1. Retinal-specific ATP-binding cassette transporter (ABCR/ABCA4) is expressed at the choroid plexus in rat brain.

    Science.gov (United States)

    Bhongsatiern, Jiraganya; Ohtsuki, Sumio; Tachikawa, Masanori; Hori, Satoko; Terasaki, Tetsuya

    2005-03-01

    ATP-binding cassette (ABC) transporter A4 is a member of the ABC transporter subfamily A which has been reported to be exclusively expressed in the retina. In contrast, a previous report has suggested a possible relationship between ABCA4 and CNS function. The purpose of the present study was to investigate the localization of ABCA4 mRNA and protein in rat brain. In situ hybridization analysis revealed that ABCA4 mRNA was localized in the lateral ventricles. RT-PCR analysis detected ABCA4 mRNA in isolated rat choroid plexus and conditionally immortalized rat choroid plexus epithelial cells (TR-CSFB). Furthermore, ABCA4 protein was also detected in the isolated rat choroid plexus at about 250 kDa by western blot analysis, and its apparent molecular size was reduced by N-glycosidase F treatment. These results suggest that glycosylated ABCA4 protein is expressed in rat choroid plexus epithelial cells. ABCA4 may play a role in the function of the blood-cerebrospinal fluid barrier and affect CSF conditions.

  2. Genome-Wide Identification, Characterization and Phylogenetic Analysis of ATP-Binding Cassette (ABC) Transporter Genes in Common Carp (Cyprinus carpio).

    Science.gov (United States)

    Liu, Xiang; Li, Shangqi; Peng, Wenzhu; Feng, Shuaisheng; Feng, Jianxin; Mahboob, Shahid; Al-Ghanim, Khalid A; Xu, Peng

    2016-01-01

    The ATP-binding cassette (ABC) gene family is considered to be one of the largest gene families in all forms of prokaryotic and eukaryotic life. Although the ABC transporter genes have been annotated in some species, detailed information about the ABC superfamily and the evolutionary characterization of ABC genes in common carp (Cyprinus carpio) are still unclear. In this research, we identified 61 ABC transporter genes in the common carp genome. Phylogenetic analysis revealed that they could be classified into seven subfamilies, namely 11 ABCAs, six ABCBs, 19 ABCCs, eight ABCDs, two ABCEs, four ABCFs, and 11 ABCGs. Comparative analysis of the ABC genes in seven vertebrate species including common carp, showed that at least 10 common carp genes were retained from the third round of whole genome duplication, while 12 duplicated ABC genes may have come from the fourth round of whole genome duplication. Gene losses were also observed for 14 ABC genes. Expression profiles of the 61 ABC genes in six common carp tissues (brain, heart, spleen, kidney, intestine, and gill) revealed extensive functional divergence among the ABC genes. Different copies of some genes had tissue-specific expression patterns, which may indicate some gene function specialization. This study provides essential genomic resources for future studies in common carp.

  3. Whole-transcriptome survey of the putative ATP-binding cassette (ABC) transporter family genes in the latex-producing laticifers of Hevea brasiliensis.

    Science.gov (United States)

    Zhiyi, Nie; Guijuan, Kang; Yu, Li; Longjun, Dai; Rizhong, Zeng

    2015-01-01

    The ATP-binding cassette (ABC) proteins or transporters constitute a large protein family in plants and are involved in many different cellular functions and processes, including solute transportation, channel regulation and molecular switches, etc. Through transcriptome sequencing, a transcriptome-wide survey and expression analysis of the ABC protein genes were carried out using the laticiferous latex from Hevea brasiliensis (rubber tree). A total of 46 putative ABC family proteins were identified in the H. brasiliensis latex. These consisted of 12 'full-size', 21 'half-size' and 13 other putative ABC proteins, and all of them showed strong conservation with their Arabidopsis thaliana counterparts. This study indicated that all eight plant ABC protein paralog subfamilies were identified in the H. brasiliensis latex, of which ABCB, ABCG and ABCI were the most abundant. Real-time quantitative reverse transcription-polymerase chain reaction assays demonstrated that gene expression of several latex ABC proteins was regulated by ethylene, jasmonic acid or bark tapping (a wound stress) stimulation, and that HbABCB15, HbABCB19, HbABCD1 and HbABCG21 responded most significantly of all to the abiotic stresses. The identification and expression analysis of the latex ABC family proteins could facilitate further investigation into their physiological involvement in latex metabolism and rubber biosynthesis by H. brasiliensis.

  4. Genome-Wide Identification, Characterization and Phylogenetic Analysis of ATP-Binding Cassette (ABC) Transporter Genes in Common Carp (Cyprinus carpio)

    Science.gov (United States)

    Peng, Wenzhu; Feng, Shuaisheng; Feng, Jianxin; Mahboob, Shahid; Al-Ghanim, Khalid A.

    2016-01-01

    The ATP-binding cassette (ABC) gene family is considered to be one of the largest gene families in all forms of prokaryotic and eukaryotic life. Although the ABC transporter genes have been annotated in some species, detailed information about the ABC superfamily and the evolutionary characterization of ABC genes in common carp (Cyprinus carpio) are still unclear. In this research, we identified 61 ABC transporter genes in the common carp genome. Phylogenetic analysis revealed that they could be classified into seven subfamilies, namely 11 ABCAs, six ABCBs, 19 ABCCs, eight ABCDs, two ABCEs, four ABCFs, and 11 ABCGs. Comparative analysis of the ABC genes in seven vertebrate species including common carp, showed that at least 10 common carp genes were retained from the third round of whole genome duplication, while 12 duplicated ABC genes may have come from the fourth round of whole genome duplication. Gene losses were also observed for 14 ABC genes. Expression profiles of the 61 ABC genes in six common carp tissues (brain, heart, spleen, kidney, intestine, and gill) revealed extensive functional divergence among the ABC genes. Different copies of some genes had tissue-specific expression patterns, which may indicate some gene function specialization. This study provides essential genomic resources for future studies in common carp. PMID:27058731

  5. Genome-Wide Identification, Characterization and Phylogenetic Analysis of ATP-Binding Cassette (ABC Transporter Genes in Common Carp (Cyprinus carpio.

    Directory of Open Access Journals (Sweden)

    Xiang Liu

    Full Text Available The ATP-binding cassette (ABC gene family is considered to be one of the largest gene families in all forms of prokaryotic and eukaryotic life. Although the ABC transporter genes have been annotated in some species, detailed information about the ABC superfamily and the evolutionary characterization of ABC genes in common carp (Cyprinus carpio are still unclear. In this research, we identified 61 ABC transporter genes in the common carp genome. Phylogenetic analysis revealed that they could be classified into seven subfamilies, namely 11 ABCAs, six ABCBs, 19 ABCCs, eight ABCDs, two ABCEs, four ABCFs, and 11 ABCGs. Comparative analysis of the ABC genes in seven vertebrate species including common carp, showed that at least 10 common carp genes were retained from the third round of whole genome duplication, while 12 duplicated ABC genes may have come from the fourth round of whole genome duplication. Gene losses were also observed for 14 ABC genes. Expression profiles of the 61 ABC genes in six common carp tissues (brain, heart, spleen, kidney, intestine, and gill revealed extensive functional divergence among the ABC genes. Different copies of some genes had tissue-specific expression patterns, which may indicate some gene function specialization. This study provides essential genomic resources for future studies in common carp.

  6. Analysis of the structural and functional roles of coupling helices in the ATP-binding cassette transporter MsbA through enzyme assays and molecular dynamics simulations.

    Science.gov (United States)

    Furuta, Tadaomi; Yamaguchi, Tomohiro; Kato, Hiroaki; Sakurai, Minoru

    2014-07-08

    ATP-binding cassette (ABC) transporters are constructed from some common structural units: the highly conserved nucleotide-binding domains (NBDs), which work as a nucleotide-dependent engine for driving substrate transport, the diverse transmembrane domains (TMDs), which create the translocation pathway, and the coupling helices (CHs), which are located at the NBD-TMD interface. Although the CHs are believed to be essential for NBD-TMD communication, their roles remain unclear. In this study, we performed enzyme assays and molecular dynamics (MD) simulations of the ABC transporter MsbA and two MsbA mutants in which the amino acid residues of one of the CHs were mutated to alanines: (i) wild type (Wt), (ii) CH1 mutant (Mt1), and (iii) CH2 mutant (Mt2). The experiments show that the CH2 mutation decreases the ATPase activity (kcat) compared with that of the Wt (a decrease of 32%), and a nearly equal degree of decrease in the ATP binding affinity (Km) was observed for both Mt1 and Mt2. The MD simulations successfully accounted for several structural and dynamical origins for these experimental observations. In addition, on the basis of collective motion and morphing analyses, we propose that the reverse-rotational motions and noddinglike motions between the NBDs and TMDs are indispensable for the conformational transition between the inward- and outward-facing conformations. In particular, CH2 is significantly important for the occurrence of the noddinglike motion. These findings provide important insights into the structure-function relationship of ABC transporters.

  7. Drug resistance is conferred on the model yeast Saccharomyces cerevisiae by expression of full-length melanoma-associated human ATP-binding cassette transporter ABCB5.

    Science.gov (United States)

    Keniya, Mikhail V; Holmes, Ann R; Niimi, Masakazu; Lamping, Erwin; Gillet, Jean-Pierre; Gottesman, Michael M; Cannon, Richard D

    2014-10-06

    ABCB5, an ATP-binding cassette (ABC) transporter, is highly expressed in melanoma cells, and may contribute to the extreme resistance of melanomas to chemotherapy by efflux of anti-cancer drugs. Our goal was to determine whether we could functionally express human ABCB5 in the model yeast Saccharomyces cerevisiae, in order to demonstrate an efflux function for ABCB5 in the absence of background pump activity from other human transporters. Heterologous expression would also facilitate drug discovery for this important target. DNAs encoding ABCB5 sequences were cloned into the chromosomal PDR5 locus of a S. cerevisiae strain in which seven endogenous ABC transporters have been deleted. Protein expression in the yeast cells was monitored by immunodetection using both a specific anti-ABCB5 antibody and a cross-reactive anti-ABCB1 antibody. ABCB5 function in recombinant yeast cells was measured by determining whether the cells possessed increased resistance to known pump substrates, compared to the host yeast strain, in assays of yeast growth. Three ABCB5 constructs were made in yeast. One was derived from the ABCB5-β mRNA, which is highly expressed in human tissues but is a truncation of a canonical full-size ABC transporter. Two constructs contained full-length ABCB5 sequences: either a native sequence from cDNA or a synthetic sequence codon-harmonized for S. cerevisiae. Expression of all three constructs in yeast was confirmed by immunodetection. Expression of the codon-harmonized full-length ABCB5 DNA conferred increased resistance, relative to the host yeast strain, to the putative substrates rhodamine 123, daunorubicin, tetramethylrhodamine, FK506, or clorgyline. We conclude that full-length ABCB5 can be functionally expressed in S. cerevisiae and confers drug resistance.

  8. An ATP Binding Cassette Transporter Mediates the Uptake of α-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates.

    Science.gov (United States)

    Ejby, Morten; Fredslund, Folmer; Andersen, Joakim Mark; Vujičić Žagar, Andreja; Henriksen, Jonas Rosager; Andersen, Thomas Lars; Svensson, Birte; Slotboom, Dirk Jan; Abou Hachem, Maher

    2016-09-16

    The molecular details and impact of oligosaccharide uptake by distinct human gut microbiota (HGM) are currently not well understood. Non-digestible dietary galacto- and gluco-α-(1,6)-oligosaccharides from legumes and starch, respectively, are preferentially fermented by mainly bifidobacteria and lactobacilli in the human gut. Here we show that the solute binding protein (BlG16BP) associated with an ATP binding cassette (ABC) transporter from the probiotic Bifidobacterium animalis subsp. lactis Bl-04 binds α-(1,6)-linked glucosides and galactosides of varying size, linkage, and monosaccharide composition with preference for the trisaccharides raffinose and panose. This preference is also reflected in the α-(1,6)-galactoside uptake profile of the bacterium. Structures of BlG16BP in complex with raffinose and panose revealed the basis for the remarkable ligand binding plasticity of BlG16BP, which recognizes the non-reducing α-(1,6)-diglycoside in its ligands. BlG16BP homologues occur predominantly in bifidobacteria and a few Firmicutes but lack in other HGMs. Among seven bifidobacterial taxa, only those possessing this transporter displayed growth on α-(1,6)-glycosides. Competition assays revealed that the dominant HGM commensal Bacteroides ovatus was out-competed by B. animalis subsp. lactis Bl-04 in mixed cultures growing on raffinose, the preferred ligand for the BlG16BP. By comparison, B. ovatus mono-cultures grew very efficiently on this trisaccharide. These findings suggest that the ABC-mediated uptake of raffinose provides an important competitive advantage, particularly against dominant Bacteroides that lack glycan-specific ABC-transporters. This novel insight highlights the role of glycan transport in defining the metabolic specialization of gut bacteria. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  9. Genome-wide analysis of ATP-binding cassette (ABC) transporters in the sweetpotato whitefly, Bemisia tabaci.

    Science.gov (United States)

    Tian, Lixia; Song, Tianxue; He, Rongjun; Zeng, Yang; Xie, Wen; Wu, Qingjun; Wang, Shaoli; Zhou, Xuguo; Zhang, Youjun

    2017-04-26

    ABC transporter superfamily is one of the largest and ubiquitous groups of proteins. Because of their role in detoxification, insect ABC transporters have gained more attention in recent years. In this study, we annotated ABC transporters from a newly sequenced sweetpotato whitefly genome. Bemisia tabaci Q biotype is an emerging global invasive species that has caused extensive damages to field crops as well as ornamental plants. A total of 55 ABC transporters containing all eight described subfamilies (A to H) were identified in the B. tabaci Q genome, including 8 ABCAs, 3 ABCBs, 6 ABCCs, 2 ABCDs, 1 ABCE, 3 ABCFs, 23 ABCGs and 9 ABCHs. In comparison to other species, subfamilies G and H in both phloem- and blood-sucking arthropods are expanded. The temporal expression profiles of these 55 ABC transporters throughout B. tabaci developmental stages and their responses to imidacloprid, a neonicotinoid insecticide, were investigated using RNA-seq analysis. Furthermore, the mRNA expression of 24 ABC transporters (44% of the total) representing all eight subfamilies was confirmed by the quantitative real-time PCR (RT-qPCR). Furthermore, mRNA expression levels estimated by RT-qPCR and RNA-seq analyses were significantly correlated (r = 0.684, p analysis of the entire repertoire of ABC transporters in B. tabaci. The identification of these ABC transporters, their temporal expression profiles during B. tabaci development, and their response to a neonicotinoid insecticide lay the foundation for functional genomic understanding of their contribution to the invasiveness of B. tabaci.

  10. Fasting Induces Nuclear Factor E2-Related Factor 2 and ATP-Binding Cassette Transporters via Protein Kinase A and Sirtuin-1 in Mouse and Human

    Science.gov (United States)

    Kulkarni, Supriya R.; Donepudi, Ajay C.; Xu, Jialin; Wei, Wei; Cheng, Qiuqiong C.; Driscoll, Maureen V.; Johnson, Delinda A.; Johnson, Jeffrey A.; Li, Xiaoling

    2014-01-01

    Abstract Aims: The purpose of this study was to determine whether 3′-5′-cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) and Sirtuin-1 (SIRT1) dependent mechanisms modulate ATP-binding Cassette (ABC) transport protein expression. ABC transport proteins (ABCC2–4) are essential for chemical elimination from hepatocytes and biliary excretion. Nuclear factor-E2 related-factor 2 (NRF2) is a transcription factor that mediates ABCC induction in response to chemical inducers and liver injury. However, a role for NRF2 in the regulation of transporter expression in nonchemical models of liver perturbation is largely undescribed. Results: Here we show that fasting increased NRF2 target gene expression through NRF2- and SIRT1–dependent mechanisms. In intact mouse liver, fasting induces NRF2 target gene expression by at least 1.5 to 5-fold. In mouse and human hepatocytes, treatment with 8-Bromoadenosine-cAMP, a cAMP analogue, increased NRF2 target gene expression and antioxidant response element activity, which was decreased by the PKA inhibitor, H-89. Moreover, fasting induced NRF2 target gene expression was decreased in liver and hepatocytes of SIRT1 liver-specific null mice and NRF2-null mice. Lastly, NRF2 and SIRT1 were recruited to MAREs and Antioxidant Response Elements (AREs) in the human ABCC2 promoter. Innovation: Oxidative stress mediated NRF2 activation is well described, yet the influence of basic metabolic processes on NRF2 activation is just emerging. Conclusion: The current data point toward a novel role of nutrient status in regulation of NRF2 activity and the antioxidant response, and indicates that cAMP/PKA and SIRT1 are upstream regulators for fasting-induced activation of the NRF2-ARE pathway. Antioxid. Redox Signal. 20, 15–30. PMID:23725046

  11. MicroRNA-20a/b regulates cholesterol efflux through post-transcriptional repression of ATP-binding cassette transporter A1.

    Science.gov (United States)

    Liang, Bin; Wang, Xin; Song, Xiaosu; Bai, Rui; Yang, Huiyu; Yang, Zhiming; Xiao, Chuanshi; Bian, Yunfei

    2017-09-01

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3' untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE -/- mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. The capacity of Listeria monocytogenes mutants with in-frame deletions in putative ATP-binding cassette transporters to form biofilms and comparison with the wild type

    Directory of Open Access Journals (Sweden)

    Marina Ceruso

    2014-02-01

    Full Text Available Listeria monocytogenes (Lm is a food-borne pathogen responsible for human listeriosis, an invasive infection with high mortality rates. Lm has developed efficient strategies for survival under stress conditions such as starvation and wide variations in temperature, pH, and osmolarity. Therefore, Lm can survive in food under multiple stress conditions. Detailed studies to determine the mode of action of this pathogen for survival under stress conditions are important to control Lm in food. It has been shown that genes encoding for ATP-binding cassette (ABC transporters are induced in Lm in food, in particular under stress conditions. Previous studies showed that these genes are involved in sensitivity to nisin, acids, and salt. The aim of this study was to determine the involvement of some ABC transporters in biofilm formation. Therefore, deletion mutants of ABC transporter genes (LMOf2365_1875 and LMOf2365_1877 were created in Lm F2365, and then were compared to the wild type for their capacity to form biofilms. Lm strain F2365 was chosen as reference since the genome is fully sequenced and furthermore this strain is particularly involved in food-borne outbreaks of listeriosis. Our results showed that DLMOf2365_1875 had an increased capacity to form biofilms compared to the wild type, indicating that LMOf2365_1875 negatively regulates biofilm formation. A deeper knowledge on the ability to form biofilms in these mutants may help in the development of intervention strategies to control Lm in food and in the environment.

  13. Polymorphisms in ATP-binding cassette transporters associated with maternal methylmercury disposition and infant neurodevelopment in mother-infant pairs in the Seychelles Child Development Study.

    Science.gov (United States)

    Engström, Karin; Love, Tanzy M; Watson, Gene E; Zareba, Grazyna; Yeates, Alison; Wahlberg, Karin; Alhamdow, Ayman; Thurston, Sally W; Mulhern, Maria; McSorley, Emeir M; Strain, J J; Davidson, Philip W; Shamlaye, Conrad F; Myers, G J; Rand, Matthew D; van Wijngaarden, Edwin; Broberg, Karin

    2016-09-01

    ATP-binding cassette (ABC) transporters have been associated with methylmercury (MeHg) toxicity in experimental animal models. To evaluate the association of single nucleotide polymorphisms (SNPs) in maternal ABC transporter genes with 1) maternal hair MeHg concentrations during pregnancy and 2) child neurodevelopmental outcomes. Nutrition Cohort 2 (NC2) is an observational mother-child cohort recruited in the Republic of Seychelles from 2008-2011. Total mercury (Hg) was measured in maternal hair growing during pregnancy as a biomarker for prenatal MeHg exposure (N=1313) (mean 3.9ppm). Infants completed developmental assessments by Bayley Scales of Infant Development II (BSID-II) at 20months of age (N=1331). Genotyping for fifteen SNPs in ABCC1, ABCC2 and ABCB1 was performed for the mothers. Seven of fifteen ABC SNPs (ABCC1 rs11075290, rs212093, and rs215088; ABCC2 rs717620; ABCB1 rs10276499, rs1202169, and rs2032582) were associated with concentrations of maternal hair Hg (pmothers with rs11075290 CC genotype (mean hair Hg 3.6ppm) scored on average 2 points lower on the Mental Development Index (MDI) and 3 points lower on the Psychomotor Development Index (PDI) than children born to mothers with TT genotype (mean hair Hg 4.7ppm) while children with the CT genotype (mean hair Hg 4.0ppm) had intermediate BSID scores. Genetic variation in ABC transporter genes was associated with maternal hair Hg concentrations. The implications for MeHg dose in the developing child and neurodevelopmental outcomes need to be further investigated. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  14. Heterocyclic cyclohexanone monocarbonyl analogs of curcumin can inhibit the activity of ATP-binding cassette transporters in cancer multidrug resistance.

    Science.gov (United States)

    Revalde, Jezrael L; Li, Yan; Hawkins, Bill C; Rosengren, Rhonda J; Paxton, James W

    2015-02-01

    Curcumin (CUR) is a phytochemical that inhibits the xenobiotic ABC efflux transporters implicated in cancer multidrug resistance (MDR), such as P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and multidrug resistance-associated proteins 1 and 5 (MRP1 and MRP5). The use of CUR in the clinic however, is complicated by its instability and poor pharmacokinetic profile. Monocarbonyl analogs of CUR (MACs) are compounds without CUR's unstable β-diketone moiety and were reported to have improved stability and in vivo disposition. Whether the MACs can be used as MDR reversal agents is less clear, as the absence of a β-diketone may negatively impact transporter inhibition. In this study, we investigated 23 heterocyclic cyclohexanone MACs for inhibitory effects against P-gp, BCRP, MRP1 and MRP5. Using flow cytometry and resistance reversal assays, we found that many of these compounds inhibited the transport activity of the ABC transporters investigated, often with much greater potency than CUR. Overall the analogs were most effective at inhibiting BCRP and we identified three compounds, A12 (2,6-bis((E)-2,5-dimethoxy-benzylidene)cyclohexanone), A13 (2,6-bis((E)-4-hydroxyl-3-methoxybenzylidene)-cyclohexanone) and B11 (3,5-bis((E)-2-fluoro-4,5-dimethoxybenzylidene)-1-methylpiperidin-4-one), as the most promising BCRP inhibitors. These compounds inhibited BCRP activity in a non-cell line, non-substrate-specific manner. Their inhibition occurred by direct transporter interaction rather than modulating protein or cell surface expression. From these results, we concluded that MACs, such as the heterocyclic cyclohexanone analogs in this study, also have potential as MDR reversal agents and may be superior alternatives to the unstable parent compound, CUR. Copyright © 2014 Elsevier Inc. All rights reserved.

  15. Phosphorus Partitioning of Soybean Lines Containing Different Mutant Alleles of Two Soybean Seed-Specific Adenosine Triphosphate-Binding Cassette Phytic Acid Transporter Paralogs

    Directory of Open Access Journals (Sweden)

    Jason D. Gillman

    2013-03-01

    Full Text Available Seed phytate is a repository of P and minerals in soybean [ (L. Merr.] seeds that limits P and mineral bioavailability for monogastric animals (e.g., humans, swine [], and poultry [especially chicken, ] due to insufficient digestive tract phytase activity. We previously identified epistatic recessive mutations affecting two paralogous adenosine triphosphate-binding cassette phytic acid transporter genes (one a nonsense mutation in and the other a missense mutation in as the molecular genetic basis in the ethyl methanesulfonate (EMS-induced mutant low phytate soybean line M153. An additional mutant low phytate line, M766, contained one single nucleotide polymorphism within the ninth intron of the locus as well as a nonsense mutation in . The objectives of this research were to clarify the genetics underlying the low phytate phenotype in line M766 and to determine P partitioning in new combinations of mutant alleles from M766 and M153. Inheritance of nonsense alleles affecting both ( genes (one from M153 and one from M766 led to the production of viable seeds that contained transgressive reductions in total seed phytate and significantly higher levels of inorganic phosphate than has been reported for nontransgenic soybean material and will allow efficient molecular selection of soybeans with even greater reductions of phytate for improved quality soybean meal.

  16. Novel Mutation in the ATP-Binding Cassette Transporter A3 (ABCA3) Encoding Gene Causes Respiratory Distress Syndrome in A Term Newborn in Southwest Iran

    Science.gov (United States)

    Rezaei, Farideh; Shafiei, Mohammad; Shariati, Gholamreza; Dehdashtian, Ali; Mohebbi, Maryam; Galehdari, Hamid

    2016-01-01

    Introduction ABCA3 glycoprotein belongs to the ATP-binding cassette (ABC) superfamily of transporters, which utilize the energy derived from hydrolysis of ATP for the translocation of a wide variety of substrates across the plasma membrane. Mutations in the ABCA3 gene are knowingly causative for fatal surfactant deficiency, particularly respiratory distress syndrome (RDS) in term babies. Case Presentation In this study, Sanger sequencing of the whole ABCA3 gene (NCBI NM_001089) was performed in a neonatal boy with severe RDS. A homozygous mutation has been identified in the patient. Parents were heterozygous for the same missense mutation GGA > AGA at position 202 in exon 6 of the ABCA3 gene (c.604G > A; p.G202R). Furthermore, 70 normal individuals have been analyzed for the mentioned change with negative results. Conclusions Regarding Human Genome Mutation Database (HGMD) and other literature recherche, the detected change is a novel mutation and has not been reported before. Bioinformatics mutation predicting tools prefer it as pathogenic. PMID:27437095

  17. IMB2026791, a Xanthone, Stimulates Cholesterol Efflux by Increasing the Binding of Apolipoprotein A-I to ATP-Binding Cassette Transporter A1

    Directory of Open Access Journals (Sweden)

    Zijian Xie

    2012-03-01

    Full Text Available It is known that the ATP-binding cassette transporter A1 (ABCA1 plays a major role in cholesterol homeostasis and high density lipoprotein (HDL metabolism. Several laboratories have demonstrated that ABCA1 binding to lipid-poor apolipoprotein A-I (apoA-I will mediate the assembly of nascent HDL and cellular cholesterol efflux, which suggests a possible receptor-ligand interaction between ABCA1 and apoA-I. In this study, a cell-based-ELISA-like high-throughput screening (HTS method was developed to identify the synthetic and natural compounds that can regulate binding activity of ABCA1 to apoA-I. The cell-based-ELISA-like high-throughput screen was conducted in a 96-well format using Chinese hamster ovary (CHO cells stably transfected with ABCA1 pIRE2-EGFP (Enhanced Green Fluorecence Protein expression vector and the known ABCA1 inhibitor glibenclamide as the antagonist control. From 2,600 compounds, a xanthone compound (IMB 2026791 was selected using this HTS assay, and it was proved as an apoA-I binding agonist to ABCA1 by a flow cytometry assay and western blot analysis. The [3H] cholesterol efflux assay of IMB2026791 treated ABCA1-CHO cells and PMA induced THP-1 macrophages (human acute monocytic leukemia cell further confirmed the compound as an accelerator of cholesterol efflux in a dose-dependent manner with an EC50 of 25.23 μM.

  18. Corticotropin-Releasing Hormone (CRH Promotes Macrophage Foam Cell Formation via Reduced Expression of ATP Binding Cassette Transporter-1 (ABCA1.

    Directory of Open Access Journals (Sweden)

    Wonkyoung Cho

    Full Text Available Atherosclerosis, the major pathology of cardiovascular disease, is caused by multiple factors involving psychological stress. Corticotropin-releasing hormone (CRH, which is released by neurosecretory cells in the hypothalamus, peripheral nerve terminals and epithelial cells, regulates various stress-related responses. Our current study aimed to verify the role of CRH in macrophage foam cell formation, the initial critical stage of atherosclerosis. Our quantitative real-time reverse transcriptase PCR (qRT-PCR, semi-quantitative reverse transcriptase PCR, and Western blot results indicate that CRH down-regulates ATP-binding cassette transporter-1 (ABCA1 and liver X receptor (LXR-α, a transcription factor for ABCA1, in murine peritoneal macrophages and human monocyte-derived macrophages. Oil-red O (ORO staining and intracellular cholesterol measurement of macrophages treated with or without oxidized LDL (oxLDL and with or without CRH (10 nM in the presence of apolipoprotein A1 (apoA1 revealed that CRH treatment promotes macrophage foam cell formation. The boron-dipyrromethene (BODIPY-conjugated cholesterol efflux assay showed that CRH treatment reduces macrophage cholesterol efflux. Western blot analysis showed that CRH-induced down-regulation of ABCA1 is dependent on phosphorylation of Akt (Ser473 induced by interaction between CRH and CRH receptor 1(CRHR1. We conclude that activation of this pathway by CRH accelerates macrophage foam cell formation and may promote stress-related atherosclerosis.

  19. Genome-wide identification of ATP-binding cassette (ABC) transporters and their roles in response to polycyclic aromatic hydrocarbons (PAHs) in the copepod Paracyclopina nana.

    Science.gov (United States)

    Jeong, Chang-Bum; Kim, Duck-Hyun; Kang, Hye-Min; Lee, Young Hwan; Kim, Hui-Su; Kim, Il-Chan; Lee, Jae-Seong

    2017-02-01

    The ATP-binding cassette (ABC) protein superfamily is one of the largest gene families and is highly conserved in all domains. The ABC proteins play roles in several biological processes, including multi-xenobiotic resistance (MXR), by functioning as transporters in the cellular membrane. They also mediate the cellular efflux of a wide range of substrates against concentration gradients. In this study, 37 ABC genes belonging to eight distinct subfamilies were identified in the marine copepod Paracyclopina nana and annotated based on a phylogenetic analysis. Also, the functions of P-glycoproteins (P-gp) and multidrug resistance-associated proteins (MRPs), conferring MXR, were verified using fluorescent substrates and specific inhibitors. The activities of MXR-mediated ABC proteins and their transcriptional level were examined in response to polyaromatic hydrocarbons (PAHs), main components of the water-accommodated fraction. This study increases the understanding of the protective role of MXR in response to PAHs over the comparative evolution of ABC gene families. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. Characterization of a lactose-responsive promoter of ATP-binding cassette (ABC) transporter gene from Lactobacillus acidophilus 05-172.

    Science.gov (United States)

    Zeng, Zhu; Zuo, Fanglei; Yu, Rui; Zhang, Bo; Ma, Huiqin; Chen, Shangwu

    2017-09-01

    A novel lactose-responsive promoter of the ATP-binding cassette (ABC) transporter gene Lba1680 of Lactobacillus acidophilus strain 05-172 isolated from a traditionally fermented dairy product koumiss was characterized. In L. acidophilus 05-172, expression of Lba1680 was induced by lactose, with lactose-induced transcription of Lba1680 being 6.1-fold higher than that induced by glucose. This is in contrast to L. acidophilus NCFM, a strain isolated from human feces, in which expression of Lba1680 and Lba1679 is induced by glucose. Both gene expression and enzyme activity assays in L. paracasei transformed with a vector containing the inducible Lba1680 promoter (PLba1680) of strain 05-172 and a heme-dependent catalase gene as reporter confirmed that PLba1680 is specifically induced by lactose. Its regulatory expression could not be repressed by glucose, and was independent of cAMP receptor protein. This lactose-responsive promoter might be used in the expression of functional genes in L. paracasei incorporated into a lactose-rich environment, such as dairy products. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  1. The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells

    Science.gov (United States)

    Castella, Barbara; Kopecka, Joanna; Sciancalepore, Patrizia; Mandili, Giorgia; Foglietta, Myriam; Mitro, Nico; Caruso, Donatella; Novelli, Francesco; Riganti, Chiara; Massaia, Massimo

    2017-01-01

    Vγ9Vδ2 T cells are activated by phosphoantigens, such as isopentenyl pyrophosphate (IPP), which is generated in the mevalonate pathway of antigen-presenting cells. IPP is released in the extracellular microenvironment via unknown mechanisms. Here we show that the ATP-binding cassette transporter A1 (ABCA1) mediates extracellular IPP release from dendritic cells (DC) in cooperation with apolipoprotein A-I (apoA-I) and butyrophilin-3A1. IPP concentrations in the supernatants are sufficient to induce Vγ9Vδ2 T cell proliferation after DC mevalonate pathway inhibition with zoledronic acid (ZA). ZA treatment increases ABCA1 and apoA-I expression via IPP-dependent LXRα nuclear translocation and PI3K/Akt/mTOR pathway inhibition. These results close the mechanistic gap in our understanding of extracellular IPP release from DC and provide a framework to fine-tune Vγ9Vδ2 T cell activation via mevalonate and PI3K/Akt/mTOR pathway modulation. PMID:28580927

  2. Suppression of the ATP-binding cassette transporter ABCC4 impairs neuroblastoma tumour growth and sensitises to irinotecan in vivo.

    Science.gov (United States)

    Murray, Jayne; Valli, Emanuele; Yu, Denise M T; Truong, Alan M; Gifford, Andrew J; Eden, Georgina L; Gamble, Laura D; Hanssen, Kimberley M; Flemming, Claudia L; Tan, Alvin; Tivnan, Amanda; Allan, Sophie; Saletta, Federica; Cheung, Leanna; Ruhle, Michelle; Schuetz, John D; Henderson, Michelle J; Byrne, Jennifer A; Norris, Murray D; Haber, Michelle; Fletcher, Jamie I

    2017-09-01

    The ATP-binding cassette transporter ABCC4 (multidrug resistance protein 4, MRP4) mRNA level is a strong predictor of poor clinical outcome in neuroblastoma which may relate to its export of endogenous signalling molecules and chemotherapeutic agents. We sought to determine whether ABCC4 contributes to development, growth and drug response in neuroblastoma in vivo. In neuroblastoma patients, high ABCC4 protein levels were associated with reduced overall survival. Inducible knockdown of ABCC4 strongly inhibited the growth of human neuroblastoma cells in vitro and impaired the growth of neuroblastoma xenografts. Loss of Abcc4 in the Th-MYCN transgenic neuroblastoma mouse model did not impact tumour formation; however, Abcc4-null neuroblastomas were strongly sensitised to the ABCC4 substrate drug irinotecan. Our findings demonstrate a role for ABCC4 in neuroblastoma cell proliferation and chemoresistance and provide rationale for a strategy where inhibition of ABCC4 should both attenuate the growth of neuroblastoma and sensitise tumours to ABCC4 chemotherapeutic substrates. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Galectin-3 silencing inhibits epirubicin-induced ATP binding cassette transporters and activates the mitochondrial apoptosis pathway via β-catenin/GSK-3β modulation in colorectal carcinoma.

    Directory of Open Access Journals (Sweden)

    Yung-Kuo Lee

    Full Text Available Multidrug resistance (MDR, an unfavorable factor compromising the treatment efficacy of anticancer drugs, involves the upregulation of ATP binding cassette (ABC transporters and induction of galectin-3 signaling. Galectin-3 plays an anti-apoptotic role in many cancer cells and regulates various pathways to activate MDR. Thus, the inhibition of galectin-3 has the potential to enhance the efficacy of the anticancer drug epirubicin. In this study, we examined the effects and mechanisms of silencing galectin-3 via RNA interference (RNAi on the β-catenin/GSK-3β pathway in human colon adenocarcinoma Caco-2 cells. Galectin-3 knockdown increased the intracellular accumulation of epirubicin in Caco-2 cells; suppressed the mRNA expression of galectin-3, β-catenin, cyclin D1, c-myc, P-glycoprotein (P-gp, MDR-associated protein (MRP 1, and MRP2; and downregulated the protein expression of P-gp, cyclin D1, galectin-3, β-catenin, c-Myc, and Bcl-2. Moreover, galectin-3 RNAi treatment significantly increased the mRNA level of GSK-3β, Bax, caspase-3, and caspase-9; remarkably increased the Bax-to-Bcl-2 ratio; and upregulated the GSK-3β and Bax protein expressions. Apoptosis was induced by galectin-3 RNAi and/or epirubicin as demonstrated by chromatin condensation, a higher sub-G1 phase proportion, and increased caspase-3 and caspase-9 activity, indicating an intrinsic/mitochondrial apoptosis pathway. Epirubicin-mediated resistance was effectively inhibited via galectin-3 RNAi treatment. However, these phenomena could be rescued after galectin-3 overexpression. We show for the first time that the silencing of galectin-3 sensitizes MDR cells to epirubicin by inhibiting ABC transporters and activating the mitochondrial pathway of apoptosis through modulation of the β-catenin/GSK-3β pathway in human colon cancer cells.

  4. Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability.

    Science.gov (United States)

    Schäfer-Somi, S; Palme, N

    2016-04-01

    The composition of seminal plasma and the localization of the ATP-binding cassette transporter A1 (ABCA1) in spermatozoa from good and bad freezers were compared to frozen-thawed spermatozoa from the same dog. Ejaculates were obtained from 31 stud dogs, and the sperm-rich fraction (SRF) was kept for analysis. One aliquot was used for the analysis of concentration, progressive motility (P; CASA), viability (V; CASA) and leucocyte count, and the analysis was performed by flow cytometry (FITC-PNA/PI), SCSA and HOST. In seminal plasma, concentration of albumin, cholesterol, calcium, inorganic phosphate, sodium, potassium, zinc and copper was measured. Semen smears were prepared and evaluated for the expression of ABCA1. The remainder of each ejaculate was frozen. After thawing, the quality assessment was repeated and further smears were prepared. According to post-thaw semen quality, dogs were assigned to good freezers (n = 20) or bad freezers (n = 11), the latter were defined as 40% morphologically abnormal sperm and/or < 50% viability. Bad freezers were older than good freezers (5.3 vs 3.4 years, p < 0.05). In bad freezers, the percentage of sperm with ABCA1 signal in the acrosome was lower (26.3% vs 35.7%, p < 0.01) and the percentage of sperm with complete loss of ABCA1 signal higher (46.7% vs 30%, p < 0.01); the percentage of dead spermatozoa was higher (36.1% vs 25.5%, p < 0.05), and the concentration of cholesterol and sodium in seminal plasma was lower than in good freezers (p < 0.05). We conclude that in thawed bad freezer sperm, an increase in acrosome damages coincided with an increased loss of cholesterol transporters and cell death, and a lower cholesterol concentration in seminal plasma. Follow-up studies revealed whether a relation exists between these findings. © 2016 Blackwell Verlag GmbH.

  5. Polycyclic Aromatic Hydrocarbons (PAHs) Mediate Transcriptional Activation of the ATP Binding Cassette Transporter ABCB6 Gene via the Aryl Hydrocarbon Receptor (AhR)*

    Science.gov (United States)

    Chavan, Hemantkumar; Krishnamurthy, Partha

    2012-01-01

    Liver is endowed with a mechanism to induce hepatic cytochromes P450 (CYP450s) in response to therapeutic drugs and environmental contaminants, leading to increased detoxification and elimination of the xenobiotics. Each CYP450 is composed of an apoprotein moiety and a heme prosthetic group, which is required for CYP450 activity. Thus, under conditions of CYP450 induction, there is a coordinate increase in heme biosynthesis to compensate for the increased expression of CYP450s. ABCB6, a mitochondrial ATP binding cassette transporter, which regulates coproporphyrinogen transport from the cytoplasm into the mitochondria to complete heme biosynthesis, represents a previously unrecognized rate-limiting step in heme biosynthesis. However, it is not known if exposure to drugs and environmental contaminants induces ABCB6 expression, to assure an adequate and apparently coordinated supply of heme for the generation of functional cytochrome holoprotein. In the present study, we demonstrate that polycyclic aromatic hydrocarbons (PAHs), the widely distributed environmental toxicants shown to induce porphyrin accumulation causing hepatic porphyria, up-regulate ABCB6 expression in both mice and humans. Using siRNA technology and Abcb6 knock-out mice, we demonstrate that PAH-mediated increase in hepatic porphyrins is compromised in the absence of ABCB6. Moreover, in vivo studies in aryl hydrocarbon receptor (AhR) knock-out mice demonstrate that PAH induction of ABCB6 is mediated by AhR. Promoter activation studies combined with electrophoretic mobility shift assay and chromatin immunoprecipitation assay demonstrate direct interactions between the AhR binding sites in the ABCB6 promoter and the AhR receptor, implicating drug activation mechanisms for ABCB6 similar to those found in inducible cytochrome P450s. These studies are the first to describe direct transcriptional activation of both mouse and human ABCB6 by xenobiotics. PMID:22761424

  6. Mutant Allele-Specific Uncoupling of PENETRATION3 Functions Reveals Engagement of the ATP-Binding Cassette Transporter in Distinct Tryptophan Metabolic Pathways1[OPEN

    Science.gov (United States)

    Lu, Xunli; Dittgen, Jan; Piślewska-Bednarek, Mariola; Molina, Antonio; Schneider, Bernd; Doubský, Jan; Schneeberger, Korbinian; Schulze-Lefert, Paul

    2015-01-01

    Arabidopsis (Arabidopsis thaliana) PENETRATION (PEN) genes quantitatively contribute to the execution of different forms of plant immunity upon challenge with diverse leaf pathogens. PEN3 encodes a plasma membrane-resident pleiotropic drug resistance-type ATP-binding cassette transporter and is thought to act in a pathogen-inducible and PEN2 myrosinase-dependent metabolic pathway in extracellular defense. This metabolic pathway directs the intracellular biosynthesis and activation of tryptophan-derived indole glucosinolates for subsequent PEN3-mediated efflux across the plasma membrane at pathogen contact sites. However, PEN3 also functions in abiotic stress responses to cadmium and indole-3-butyric acid (IBA)-mediated auxin homeostasis in roots, raising the possibility that PEN3 exports multiple functionally unrelated substrates. Here, we describe the isolation of a pen3 allele, designated pen3-5, that encodes a dysfunctional protein that accumulates in planta like wild-type PEN3. The specific mutation in pen3-5 uncouples PEN3 functions in IBA-stimulated root growth modulation, callose deposition induced with a conserved peptide epitope of bacterial flagellin (flg22), and pathogen-inducible salicylic acid accumulation from PEN3 activity in extracellular defense, indicating the engagement of multiple PEN3 substrates in different PEN3-dependent biological processes. We identified 4-O-β-d-glucosyl-indol-3-yl formamide (4OGlcI3F) as a pathogen-inducible, tryptophan-derived compound that overaccumulates in pen3 leaf tissue and has biosynthesis that is dependent on an intact PEN2 metabolic pathway. We propose that a precursor of 4OGlcI3F is the PEN3 substrate in extracellular pathogen defense. These precursors, the shared indole core present in IBA and 4OGlcI3F, and allele-specific uncoupling of a subset of PEN3 functions suggest that PEN3 transports distinct indole-type metabolites in distinct biological processes. PMID:26023163

  7. Conceptual design of divertor cassette handling by remote handling system for JT-60SA

    International Nuclear Information System (INIS)

    Hayashi, Takao; Sakurai, Shinji; Masaki, Kei; Tamai, Hiroshi; Yoshida, Kiyoshi; Matsukawa, Makoto

    2007-01-01

    The JT-60SA aims to contribute and supplement ITER toward DEMO reactor based on tokamak concept. One of the features of JT-60SA is its high power long pulse heating, causing the large annual neutron fluence. Because the expected dose rate at the vacuum vessel (VV) may exceed 1 mSv/hr after 10 years operation and three month cooling, the human access inside the VV is prohibited. Therefore a remote handling (RH) system is necessary for the maintenance and repair of in-vessel components. This paper described the RH system of JT-60SA, especially the expansion of the RH rail and exchange of the divertor modules. The RH rail is divided into nine and three-point mounting. The nine sections can cover 225 degrees in toroidal direction. A divertor module, which is 10 degrees wide in toroidal direction and weighs 500kg itself due to the limitations of port width and handling weight, can be exchanged by heavy weight manipulator (HWM). The HWM brings the divertor module to the front of the other RH port, which is used for supporting the rail and/or carrying in and out equipments. Then another RH device receives and brings out the module by a pallet installed from outside the VV. (author)

  8. Cosmo Cassette: A Microfluidic Microgravity Microbial System For Synthetic Biology Unit Tests and Satellite Missions

    Science.gov (United States)

    Berliner, Aaron J.

    2013-01-01

    Although methods in the design-build-test life cycle of the synthetic biology field have grown rapidly, the expansion has been non-uniform. The design and build stages in development have seen innovations in the form of biological CAD and more efficient means for building DNA, RNA, and other biological constructs. The testing phase of the cycle remains in need of innovation. Presented will be both a theoretical abstraction of biological measurement and a practical demonstration of a microfluidics-based platform for characterizing synthetic biological phenomena. Such a platform demonstrates a design of additive manufacturing (3D printing) for construction of a microbial fuel cell (MFC) to be used in experiments carried out in space. First, the biocompatibility of the polypropylene chassis will be demonstrated. The novel MFCs will be cheaper, and faster to make and iterate through designs. The novel design will contain a manifold switchingdistribution system and an integrated in-chip set of reagent reservoirs fabricated via 3D printing. The automated nature of the 3D printing yields itself to higher resolution switching valves and leads to smaller sized payloads, lower cost, reduced power and a standardized platform for synthetic biology unit tests on Earth and in space. It will be demonstrated that the application of unit testing in synthetic biology will lead to the automatic construction and validation of desired constructs. Unit testing methodologies offer benefits of preemptive problem identification, change of facility, simplicity of integration, ease of documentation, and separation of interface from implementation, and automated design.

  9. SALL4, a stem cell factor, affects the side population by regulation of the ATP-binding cassette drug transport genes.

    Directory of Open Access Journals (Sweden)

    Ha-Won Jeong

    2011-04-01

    Full Text Available Our previous work shows that the stem cell factor SALL4 plays a central role in embryonic and leukemic stem cells. In this study, we report that SALL4 expression was higher in drug resistant primary acute myeloid leukemic patients than those from drug-responsive cases. In addition, while overexpression of SALL4 led to drug resistance in cell lines, cells with decreased SALL4 expression were more sensitive to drug treatments than the parental cells. This led to our investigation of the implication of SALL4 in drug resistance and its role in side population (SP cancer stem cells. SALL4 expression was higher in SP cells compared to non-SP cells by 2-4 fold in various malignant hematopoietic cell lines. Knocking down of SALL4 in isolated SP cells resulted in a reduction of SP cells, indicating that SALL4 is required for their self-renewal. The SP phenotype is known to be mediated by members of the ATP-binding cassette (ABC drug transport protein family, such as ABCG2 and ABCA3. Using chromatin-immunoprecipitation (ChIP, quantitative reverse transcription polymerase chain reaction (qRT-PCR and electrophoretic mobility shift assay(EMSA, we demonstrated that SALL4 was able to bind to the promoter region of ABCA3 and activate its expression while regulating the expression of ABCG2 indirectly. Furthermore, SALL4 expression was positively correlated to those of ABCG2 and ABCA3 in primary leukemic patient samples. Taken together, our results suggest a novel role for SALL4 in drug sensitivity, at least in part through the maintenance of SP cells, and therefore may be responsible for drug-resistance in leukemia. We are the first to demonstrate a direct link between stem cell factor SALL4, SP and drug resistance in leukemia.

  10. Deficiency of ATP-binding cassette transporters A1 and G1 in macrophages increases inflammation and accelerates atherosclerosis in mice.

    Science.gov (United States)

    Westerterp, Marit; Murphy, Andrew J; Wang, Mi; Pagler, Tamara A; Vengrenyuk, Yuliya; Kappus, Mojdeh S; Gorman, Darren J; Nagareddy, Prabhakara R; Zhu, Xuewei; Abramowicz, Sandra; Parks, John S; Welch, Carrie; Fisher, Edward A; Wang, Nan; Yvan-Charvet, Laurent; Tall, Alan R

    2013-05-24

    Plasma high-density lipoprotein levels are inversely correlated with atherosclerosis. Although it is widely assumed that this is attributable to the ability of high-density lipoprotein to promote cholesterol efflux from macrophage foam cells, direct experimental support for this hypothesis is lacking. To assess the role of macrophage cholesterol efflux pathways in atherogenesis. We developed mice with efficient deletion of the ATP-binding cassette transporters A1 and G1 (ABCA1 and ABCG1) in macrophages (MAC-ABC(DKO) mice) but not in hematopoietic stem or progenitor populations. MAC-ABC(DKO) bone marrow (BM) was transplanted into Ldlr(-/-) recipients. On the chow diet, these mice had similar plasma cholesterol and blood monocyte levels but increased atherosclerosis compared with controls. On the Western-type diet, MAC-ABC(DKO) BM-transplanted Ldlr(-/-) mice had disproportionate atherosclerosis, considering they also had lower very low-density lipoprotein/low-density lipoprotein cholesterol levels than controls. ABCA1/G1-deficient macrophages in lesions showed increased inflammatory gene expression. Unexpectedly, Western-type diet-fed MAC-ABC(DKO) BM-transplanted Ldlr(-/-) mice displayed monocytosis and neutrophilia in the absence of hematopoietic stem and multipotential progenitor cells proliferation. Mechanistic studies revealed increased expressions of machrophage colony stimulating factor and granulocyte colony stimulating factor in splenic macrophage foam cells, driving BM monocyte and neutrophil production. These studies show that macrophage deficiency of ABCA1/G1 is proatherogenic likely by promoting plaque inflammation and uncover a novel positive feedback loop in which cholesterol-laden splenic macrophages signal BM progenitors to produce monocytes, with suppression by macrophage cholesterol efflux pathways.

  11. Insulin-like Growth Factor 1 Regulates the Expression of ATP-Binding Cassette Transporter A1 in Pancreatic Beta Cells.

    Science.gov (United States)

    Lyu, J; Imachi, H; Iwama, H; Zhang, H; Murao, K

    2016-05-01

    ATP-binding cassette transporter A1 (ABCA1) in pancreatic beta cells influences insulin secretion and cholesterol homeostasis. The present study investigates whether insulin-like growth factor 1 (IGF-1), which mediates stimulation of ABCA1 gene expression, could also interfere with the phosphatidylinositol 3-kinase (PI3-K) cascade.ABCA1 expression was examined by real-time polymerase chain reaction (PCR), Western blot analysis, and a reporter gene assay in rat insulin-secreting INS-1 cells incubated with IGF-1. The binding of forkhead box O1 (FoxO1) protein to the ABCA1 promoter was assessed by a chromatin immunoprecipitation (ChIP) assay. ABCA1 protein levels increased in response to rising concentrations of IGF-1. Real-time PCR analysis showed a significant increase in ABCA1 mRNA expression. However, both effects were suppressed after silencing the IGF-1 receptor. In parallel with its effect on endogenous ABCA1 mRNA levels, IGF-1 induced the activity of a reporter construct containing the ABCA1 promoter, while it was abrogated by LY294002, a specific inhibitor of PI3-K. Constitutively active Akt stimulated activity of the ABCA1 promoter, and a dominant-negative mutant of Akt or mutagenesis of the FoxO1 response element in the ABCA1 promoter abolished the ability of IGF-1 to stimulate promoter activity. A ChIP assay showed that FoxO1 mediated its transcriptional activity by directly binding to the ABCA1 promoter region. The knockdown of FoxO1 disrupted the effect of IGF-1 on ABCA1 expression. Furthermore, IGF-1 promoted cholesterol efflux and reduced the pancreatic lipotoxicity. These results demonstrate that the PI3-K/Akt/FoxO1 pathway contributes to the regulation of ABCA1 expression in response to IGF-1 stimulation. © Georg Thieme Verlag KG Stuttgart · New York.

  12. A mutation within the extended X loop abolished substrate-induced ATPase activity of the human liver ATP-binding cassette (ABC) transporter MDR3.

    Science.gov (United States)

    Kluth, Marianne; Stindt, Jan; Dröge, Carola; Linnemann, Doris; Kubitz, Ralf; Schmitt, Lutz

    2015-02-20

    The human multidrug resistance protein 3 (MDR3/ABCB4) belongs to the ubiquitous family of ATP-binding cassette (ABC) transporters and is located in the canalicular membrane of hepatocytes. There it flops the phospholipids of the phosphatidylcholine (PC) family from the inner to the outer leaflet. Here, we report the characterization of wild type MDR3 and the Q1174E mutant, which was identified previously in a patient with progressive familial intrahepatic cholestasis type 3 (PFIC-3). We expressed different variants of MDR3 in the yeast Pichia pastoris, purified the proteins via tandem affinity chromatography, and determined MDR3-specific ATPase activity in the presence or absence of phospholipids. The ATPase activity of wild type MDR3 was stimulated 2-fold by liver PC or 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine lipids. Furthermore, the cross-linking of MDR3 with a thiol-reactive fluorophore blocked ATP hydrolysis and exhibited no PC stimulation. Similarly, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin lipids did not induce an increase of wild type MDR3 ATPase activity. The phosphate analogues beryllium fluoride and aluminum fluoride led to complete inhibition of ATPase activity, whereas orthovanadate inhibited exclusively the PC-stimulated ATPase activity of MDR3. The Q1174E mutation is located in the nucleotide-binding domain in direct proximity of the leucine of the ABC signature motif and extended the X loop, which is found in ABC exporters. Our data on the Q1174E mutant demonstrated basal ATPase activity, but PC lipids were incapable of stimulating ATPase activity highlighting the role of the extended X loop in the cross-talk of the nucleotide-binding domain and the transmembrane domain. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  13. Nicotiana plumbaginifolia plants silenced for the ATP-binding cassette transporter gene NpPDR1 show increased susceptibility to a group of fungal and oomycete pathogens.

    Science.gov (United States)

    Bultreys, Alain; Trombik, Tomasz; Drozak, Anna; Boutry, Marc

    2009-09-01

    SUMMARY The behaviour of Nicotiana plumbaginifolia plants silenced for the ATP-binding cassette transporter gene NpPDR1 was investigated in response to fungal and oomycete infections. The importance of NpPDR1 in plant defence was demonstrated for two organs in which NpPDR1 is constitutively expressed: the roots and the petal epidermis. The roots of the plantlets of two lines silenced for NpPDR1 expression were clearly more sensitive than those of controls to the fungal pathogens Botrytis cinerea, Fusarium oxysporum sp., F. oxysporum f. sp. nicotianae, F. oxysporum f. sp. melonis and Rhizoctonia solani, as well as to the oomycete pathogen Phytophthora nicotianae race 0. The Ph gene-linked resistance of N. plumbaginifolia to P. nicotianae race 0 was totally ineffective in NpPDR1-silenced lines. In addition, the petals of the NpPDR1-silenced lines were spotted 15%-20% more rapidly by B. cinerea than were the controls. The rapid induction (after 2-4 days) of NpPDR1 expression in N. plumbaginifolia and N. tabacum mature leaves in response to pathogen presence was demonstrated for the first time with fungi and one oomycete: R. solani, F. oxysporum and P. nicotianae. With B. cinerea, such rapid expression was not observed in healthy mature leaves. NpPDR1 expression was not observed during latent infections of B. cinerea in N. plumbaginifolia and N. tabacum, but was induced when conditions facilitated B. cinerea development in leaves, such as leaf ageing or an initial root infection. This work demonstrates the increased sensitivity of NpPDR1-silenced N. plumbaginifolia plants to all of the fungal and oomycete pathogens investigated.

  14. Radiographic film cassette unloading apparatus

    International Nuclear Information System (INIS)

    Stievenart, E.F.; Plessers, H.S.; Neujens, G.J.

    1980-01-01

    Apparatus for unloading cassettes, containing exposed radiographic films, has means for unfastening the cassettes, an inclined pathway for gravity feeding and rotating feed members (rollers or belts) to propel the films into the processor. (UK)

  15. Transportation System Requirements Document

    International Nuclear Information System (INIS)

    1993-09-01

    This Transportation System Requirements Document (Trans-SRD) describes the functions to be performed by and the technical requirements for the Transportation System to transport spent nuclear fuel (SNF) and high-level radioactive waste (HLW) from Purchaser and Producer sites to a Civilian Radioactive Waste Management System (CRWMS) site, and between CRWMS sites. The purpose of this document is to define the system-level requirements for Transportation consistent with the CRWMS Requirement Document (CRD). These requirements include design and operations requirements to the extent they impact on the development of the physical segments of Transportation. The document also presents an overall description of Transportation, its functions, its segments, and the requirements allocated to the segments and the system-level interfaces with Transportation. The interface identification and description are published in the CRWMS Interface Specification

  16. Regulation of Human γδ T Cells by BTN3A1 Protein Stability and ATP-Binding Cassette Transporters

    Directory of Open Access Journals (Sweden)

    David A. Rhodes

    2018-04-01

    Full Text Available Activation of human Vγ9/Vδ2 T cells by “phosphoantigens” (pAg, the microbial metabolite (E-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP and the endogenous isoprenoid intermediate isopentenyl pyrophosphate, requires expression of butyrophilin BTN3A molecules by presenting cells. However, the precise mechanism of activation of Vγ9/Vδ2 T cells by BTN3A molecules remains elusive. It is not clear what conformation of the three BTN3A isoforms transmits activation signals nor how externally delivered pAg accesses the cytosolic B30.2 domain of BTN3A1. To approach these problems, we studied two HLA haplo-identical HeLa cell lines, termed HeLa-L and HeLa-M, which showed marked differences in pAg-dependent stimulation of Vγ9/Vδ2 T cells. Levels of IFN-γ secretion by Vγ9/Vδ2 T cells were profoundly increased by pAg loading, or by binding of the pan-BTN3A specific agonist antibody CD277 20.1, in HeLa-M compared to HeLa-L cells. IL-2 production from a murine hybridoma T cell line expressing human Vγ9/Vδ2 T cell receptor (TCR transgenes confirmed that the differential responsiveness to HeLa-L and HeLa-M was TCR dependent. By tissue typing, both HeLa lines were shown to be genetically identical and full-length transcripts of the three BTN3A isoforms were detected in equal abundance with no sequence variation. Expression of BTN3A and interacting molecules, such as periplakin or RhoB, did not account for the functional variation between HeLa-L and HeLa-M cells. Instead, the data implicate a checkpoint controlling BTN3A1 stability and protein trafficking, acting at an early time point in its maturation. In addition, plasma membrane profiling was used to identify proteins upregulated in HMB-PP-treated HeLa-M. ABCG2, a member of the ATP-binding cassette (ABC transporter family was the most significant candidate, which crucially showed reduced expression in HeLa-L. Expression of a subset of ABC transporters, including ABCA1 and ABCG1, correlated

  17. Regulation of Human γδ T Cells by BTN3A1 Protein Stability and ATP-Binding Cassette Transporters

    Science.gov (United States)

    Rhodes, David A.; Chen, Hung-Chang; Williamson, James C.; Hill, Alfred; Yuan, Jack; Smith, Sam; Rhodes, Harriet; Trowsdale, John; Lehner, Paul J.; Herrmann, Thomas; Eberl, Matthias

    2018-01-01

    Activation of human Vγ9/Vδ2 T cells by “phosphoantigens” (pAg), the microbial metabolite (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP) and the endogenous isoprenoid intermediate isopentenyl pyrophosphate, requires expression of butyrophilin BTN3A molecules by presenting cells. However, the precise mechanism of activation of Vγ9/Vδ2 T cells by BTN3A molecules remains elusive. It is not clear what conformation of the three BTN3A isoforms transmits activation signals nor how externally delivered pAg accesses the cytosolic B30.2 domain of BTN3A1. To approach these problems, we studied two HLA haplo-identical HeLa cell lines, termed HeLa-L and HeLa-M, which showed marked differences in pAg-dependent stimulation of Vγ9/Vδ2 T cells. Levels of IFN-γ secretion by Vγ9/Vδ2 T cells were profoundly increased by pAg loading, or by binding of the pan-BTN3A specific agonist antibody CD277 20.1, in HeLa-M compared to HeLa-L cells. IL-2 production from a murine hybridoma T cell line expressing human Vγ9/Vδ2 T cell receptor (TCR) transgenes confirmed that the differential responsiveness to HeLa-L and HeLa-M was TCR dependent. By tissue typing, both HeLa lines were shown to be genetically identical and full-length transcripts of the three BTN3A isoforms were detected in equal abundance with no sequence variation. Expression of BTN3A and interacting molecules, such as periplakin or RhoB, did not account for the functional variation between HeLa-L and HeLa-M cells. Instead, the data implicate a checkpoint controlling BTN3A1 stability and protein trafficking, acting at an early time point in its maturation. In addition, plasma membrane profiling was used to identify proteins upregulated in HMB-PP-treated HeLa-M. ABCG2, a member of the ATP-binding cassette (ABC) transporter family was the most significant candidate, which crucially showed reduced expression in HeLa-L. Expression of a subset of ABC transporters, including ABCA1 and ABCG1, correlated with

  18. Intelligent Freigth Transport Systems

    DEFF Research Database (Denmark)

    Overø, Helene Martine; Larsen, Allan; Røpke, Stefan

    2009-01-01

    is to enhance the efficiency and lower the environmental impact in freight transport. In this paper, a pilot project involving real-time waste collection at a Danish waste collection company is described, and a solution approach is proposed. The problem corresponds to the dynamic version of the waste collection......The Danish innovation project entitled “Intelligent Freight Transport Systems” aims at developing prototype systems integrating public intelligent transport systems (ITS) with the technology in vehicles and equipment as well as the IT-systems at various transport companies. The objective...

  19. TRANSPORTATION SYSTEM REQUIREMENTS DOCUMENT

    International Nuclear Information System (INIS)

    2004-01-01

    This document establishes the Transportation system requirements for the U.S. Department of Energy's (DOE's) Civilian Radioactive Waste Management System (CRWMS). These requirements are derived from the Civilian Radioactive Waste Management System Requirements Document (CRD). The Transportation System Requirements Document (TSRD) was developed in accordance with LP-3.1Q-OCRWM, Preparation, Review, and Approval of Office of National Transportation Level-2 Baseline Requirements. As illustrated in Figure 1, the TSRD forms a part of the DOE Office of Civilian Radioactive Waste Management (OCRWM) Technical Baseline

  20. Electrical railway transportation systems

    CERN Document Server

    Brenna, Morris; Zaninelli, Dario

    2018-01-01

    Allows the reader to deepen their understanding of various technologies for both fixed power supply installations of railway systems and for railway rolling stock. This book explores the electric railway systems that play a crucial role in the mitigation of congestion and pollution caused by road traffic. It is divided into two parts: the first covering fixed power supply systems, and the second concerning the systems for railway rolling stock. In particular, after a historical introduction to the framework of technological solutions in current use, the authors investigate electrification systems for the power supply of rail vehicles, trams, and subways. Electrical Railway Transportation Systems explores the direct current systems used throughout the world for urban and suburban transport, which are also used in various countries for regional transport. It provides a study of alternating current systems, whether for power supply frequency or for special railway frequency, that are used around the world for ...

  1. Polymorphisms in ATP-binding cassette transporter genes and interaction with diet and life style factors in relation to colorectal cancer in a Danish prospective case-cohort study

    DEFF Research Database (Denmark)

    Kopp, Tine Iskov; Andersen, Vibeke; Tjonneland, Anne

    2015-01-01

    to assess whether polymorphisms in ABCB1, ABCC2 and ABCG2 were associated with risk of colorectal cancer (CRC) and to investigate gene-environment (dietary factors, smoking and use of non-steroidal anti-inflammatory drugs) and gene-gene interactions between previously studied polymorphisms in IL1B and IL10......The ATP-binding cassette (ABC) transporter family transports various molecules across the enterocytes in the gut protecting the intestine against potentially harmful substances. Moreover, ABC transporters are involved in mucosal immune defence through interaction with cytokines. The study aimed...... of the polymorphisms were associated with CRC, but ABCB1 and ABCG2 haplotypes were associated with risk of CRC. ABCB1/rs1045642 interacted with intake of cereals and fiber (p-Value for interaction (Pint) = 0.001 and 0.01, respectively). In a three-way analysis, both ABCB1/rs1045642 and ABCG2/rs2231137 in combination...

  2. X-ray film cassette and method of making

    International Nuclear Information System (INIS)

    1980-01-01

    An x-ray film cassette which is capable of providing forces on the film that vary across the surface of the cassette is described. Methods of manufacture are discussed. The system is of particular use when large area films are used in conjunction with intensifying screens. (U.K.)

  3. Lightweight Monorail Transport System

    Science.gov (United States)

    Weir, Harold F.; Wood, Kenneth E.; Strecker, Myron T.

    1987-01-01

    Report proposes monorail transportation system for zero-gravity environment. System carries materials and parts between locations on space station. Includes tubular rails instead of open channels usually found in overhead conveyor systems. Since resistance to torque of closed tube greater than that of open channel for same amount of material, tubular monorail designed for higher loads or for greater spacing between support points.

  4. Cassette pontoon bridge of high mobility

    Directory of Open Access Journals (Sweden)

    Krzysztof KOSIUCZENKO

    2011-01-01

    Full Text Available Looking through the known and used buoyant systems, it can be remarked that the single buoyant segments are the stiff objects made of steel or plastic with variable dimensions and a complex construction. The ready to use buoyant segments, that assure the proper displacement, must have the factory leak-tightness. They take up a big transportation volume and need the assurance of the suitably abundant means of transport. Usually the heavy wheeled vehicles are needed because of high own mass of buoyant segment and large gauges. The exploitation of such constructions is very expensive. A cassette pontoon bridge, presented in this paper, is the proposition of the increase of the mobility of construction. The decrease of the single buoyant segment dimensions with the assurance of the capacity leads that more segments fit into in the same dimensions of the loading compartment of the vehicle and storage accommodation. The application of standardized joints assures the assembly efficiency with not numerous crew.

  5. Transportable criticality alarm system

    International Nuclear Information System (INIS)

    Clem, W.E.

    1988-09-01

    The Transportable Criticality Alarm System was developed at the Hanford Site in 1982 to comply with the requirements of US Department of Energy Order DOE 5480.1, 12/18/80, and ANSI/ANS-8.3- 1979. The portable unit that it replaced failed to comply with the new requirements in that it did not provide the necessary warning of malfunctions, nor did it provide the Hanford Site standard criticality alarm signal. Modern technology allowed the Transportable Criticality Alarm System to comply with the criticality requirements cited and to incorporate other features that make it more usable, maintainable, and reliable. The Transportable Criticality Alarm System (TCAS) provides temporary criticality coverage in manned areas where the facility criticality alarm system is not operable. This gamma radiation-sensitive system has been in use for the past 6 yr at the Hanford Site. 2 refs., 4 figs., 1 tab

  6. Suppression of c-Myc is involved in multi-walled carbon nanotubes' down-regulation of ATP-binding cassette transporters in human colon adenocarcinoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Zhaojing [Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan (China); Xu, Yonghong [Institute of Ophthalmological Research, Department of Ophthalmology, Renmin Hospital of Wuhan University, 430060 Wuhan (China); Meng, Xiangning [School of Materials and Metallurgy, Northeastern University, Shenyang 110819 (China); Watari, Fumio [Department of Biomedical, Dental Materials and Engineering, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586 (Japan); Liu, Hudan, E-mail: hudanliu@hust.edu.cn [Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan (China); Chen, Xiao, E-mail: mornsmile@yahoo.com [Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan (China)

    2015-01-01

    Over-expression of ATP-binding cassette (ABC) transporters, a large family of integral membrane proteins that decrease cellular drug uptake and accumulation by active extrusion, is one of the major causes of cancer multi-drug resistance (MDR) that frequently leads to failure of chemotherapy. Carbon nanotubes (CNTs)-based drug delivery devices hold great promise in enhancing the efficacy of cancer chemotherapy. However, CNTs' effects on the ABC transporters remain under-investigated. In this study, we found that multiwalled carbon nanotubes (MWCNTs) reduced transport activity and expression of ABC transporters including ABCB1/Pgp and ABCC4/MRP4 in human colon adenocarcinoma Caco-2 cells. Proto-oncogene c-Myc, which directly regulates ABC gene expression, was concurrently decreased in MWCNT-treated cells and forced over-expression of c-Myc reversed MWCNTs' inhibitory effects on ABCB1 and ABCC4 expression. MWCNT-cell membrane interaction and cell membrane oxidative damage were observed. However, antioxidants such as vitamin C, β-mecaptoethanol and dimethylthiourea failed to antagonize MWCNTs' down-regulation of ABC transporters. These data suggest that MWCNTs may act on c-Myc, but not through oxidative stress, to down-regulate ABC transporter expression. Our findings thus shed light on CNTs' novel cellular effects that may be utilized to develop CNTs-based drug delivery devices to overcome ABC transporter-mediated cancer chemoresistance.

  7. Blood Sample Transportation by Pneumatic Transportation Systems

    DEFF Research Database (Denmark)

    Nybo, Mads; Lund, Merete E; Titlestad, Kjell

    2018-01-01

    BACKGROUND: Pneumatic transportation systems (PTSs) are increasingly used for transportation of blood samples to the core laboratory. Many studies have investigated the impact of these systems on different types of analyses, but to elucidate whether PTSs in general are safe for transportation...... analysis, and the hemolysis index). CONCLUSIONS: Owing to their high degree of heterogeneity, the retrieved studies were unable to supply evidence for the safety of using PTSs for blood sample transportation. In consequence, laboratories need to measure and document the actual acceleration forces...

  8. Lunar transportation system

    Science.gov (United States)

    1993-07-01

    The University Space Research Association (USRA) requested the University of Minnesota Spacecraft Design Team to design a lunar transportation infrastructure. This task was a year long design effort culminating in a complete conceptual design and presentation at Johnson Space Center. The mission objective of the design group was to design a system of vehicles to bring a habitation module, cargo, and crew to the lunar surface from LEO and return either or both crew and cargo safely to LEO while emphasizing component commonality, reusability, and cost effectiveness. During the course of the design, the lunar transportation system (LTS) has taken on many forms. The final design of the system is composed of two vehicles, a lunar transfer vehicle (LTV) and a lunar excursion vehicle (LEV). The LTV serves as an efficient orbital transfer vehicle between the earth and the moon while the LEV carries crew and cargo to the lunar surface. Presented in the report are the mission analysis, systems layout, orbital mechanics, propulsion systems, structural and thermal analysis, and crew systems, avionics, and power systems for this lunar transportation concept.

  9. Rail transport systems approach

    CERN Document Server

    2017-01-01

    This book shows how the systems approach is employed by scientists in various countries to solve specific problems concerning railway transport. In particular, the book describes the experiences of scientists from Romania, Germany, the Czech Republic, the UK, Russia, Ukraine, Lithuania and Poland. For many of these countries there is a problem with the historical differences between the railways. In particular, there are railways with different rail gauges, with different signaling and communication systems, with different energy supplies and, finally, with different political systems, which are reflected in the different approaches to the management of railway economies. The book’s content is divided into two main parts, the first of which provides a systematic analysis of individual means of providing and maintaining rail transport. In turn, the second part addresses infrastructure and management development, with particular attention to security issues. Though primarily written for professionals involved...

  10. Heat transport system

    International Nuclear Information System (INIS)

    Pierce, B.L.

    1978-01-01

    A heat transport system of small size which can be operated in any orientation consists of a coolant loop containing a vaporizable liquid as working fluid and includes in series a vaporizer, a condenser and two one-way valves and a pressurizer connected to the loop between the two valves. The pressurizer may be divided into two chambers by a flexible diaphragm, an inert gas in one chamber acts as a pneumatic spring for the system. This system is suitable for use in a nuclear-powered artificial heart

  11. SUBSURFACE EMPLACEMENT TRANSPORTATION SYSTEM

    International Nuclear Information System (INIS)

    Wilson, T.; Novotny, R.

    1999-01-01

    The objective of this analysis is to identify issues and criteria that apply to the design of the Subsurface Emplacement Transportation System (SET). The SET consists of the track used by the waste package handling equipment, the conductors and related equipment used to supply electrical power to that equipment, and the instrumentation and controls used to monitor and operate those track and power supply systems. Major considerations of this analysis include: (1) Operational life of the SET; (2) Geometric constraints on the track layout; (3) Operating loads on the track; (4) Environmentally induced loads on the track; (5) Power supply (electrification) requirements; and (6) Instrumentation and control requirements. This analysis will provide the basis for development of the system description document (SDD) for the SET. This analysis also defines the interfaces that need to be considered in the design of the SET. These interfaces include, but are not limited to, the following: (1) Waste handling building; (2) Monitored Geologic Repository (MGR) surface site layout; (3) Waste Emplacement System (WES); (4) Waste Retrieval System (WRS); (5) Ground Control System (GCS); (6) Ex-Container System (XCS); (7) Subsurface Electrical Distribution System (SED); (8) MGR Operations Monitoring and Control System (OMC); (9) Subsurface Facility System (SFS); (10) Subsurface Fire Protection System (SFR); (11) Performance Confirmation Emplacement Drift Monitoring System (PCM); and (12) Backfill Emplacement System (BES)

  12. Magnetic type transportation system

    International Nuclear Information System (INIS)

    Kobama, Masao.

    1981-01-01

    Purpose: To enable automatic transportation of nuclear substances with optional setting for the transportation distance, even for a long distance, facilitating the automation of the transportation and decreasing the space for the installation of a direction converging section of the transporting path. Constitution: A transporting vehicle having a pair of permanent magnets or ferromagnetic bodies mounted with a predetermined gap to each other along the transporting direction is provided in the transporting path including a bent direction change section for transporting specimens such as nuclear materials, and a plurality of driving vehicles having permanent magnets or ferromagnetic bodies for magnetically attracting the transporting vehicle from outside of the transporting path are arranged to the outside of the transporting path. At least one of the driving vehicles is made to run along the transporting direction of the transporting path by a driving mechanism incorporating running section such as an endless chain to drive the transportation vehicle, and the transporting vehicle is successively driven by each of the driving mechanisms. (Kawakami, Y.)

  13. Structural elucidation of transmembrane domain zero (TMD0) of EcdL: A multidrug resistance-associated protein (MRP) family of ATP-binding cassette transporter protein revealed by atomistic simulation.

    Science.gov (United States)

    Bera, Krishnendu; Rani, Priyanka; Kishor, Gaurav; Agarwal, Shikha; Kumar, Antresh; Singh, Durg Vijay

    2017-09-20

    ATP-Binding cassette (ABC) transporters play an extensive role in the translocation of diverse sets of biologically important molecules across membrane. EchnocandinB (antifungal) and EcdL protein of Aspergillus rugulosus are encoded by the same cluster of genes. Co-expression of EcdL and echinocandinB reflects tightly linked biological functions. EcdL belongs to Multidrug Resistance associated Protein (MRP) subfamily of ABC transporters with an extra transmembrane domain zero (TMD0). Complete structure of MRP subfamily comprising of TMD0 domain, at atomic resolution is not known. We hypothesized that the transportation of echonocandinB is mediated via EcdL protein. Henceforth, it is pertinent to know the topological arrangement of TMD0, with other domains of protein and its possible role in transportation of echinocandinB. Absence of effective template for TMD0 domain lead us to model by I-TASSER, further structure has been refined by multiple template modelling using homologous templates of remaining domains (TMD1, NBD1, TMD2, NBD2). The modelled structure has been validated for packing, folding and stereochemical properties. MD simulation for 0.1 μs has been carried out in the biphasic environment for refinement of modelled protein. Non-redundant structures have been excavated by clustering of MD trajectory. The structural alignment of modelled structure has shown Z-score -37.9; 31.6, 31.5 with RMSD; 2.4, 4.2, 4.8 with ABC transporters; PDB ID 4F4C, 4M1 M, 4M2T, respectively, reflecting the correctness of structure. EchinocandinB has been docked to the modelled as well as to the clustered structures, which reveals interaction of echinocandinB with TMD0 and other TM helices in the translocation path build of TMDs.

  14. The beam transport system

    International Nuclear Information System (INIS)

    1986-01-01

    The first proton beams have been transported along the transfer beamline and the diagnostic components have thus been used and tested under real operating conditions. The various electronic systems have been linked to the control system and the equipment can now be operated from the control console. The performance of the diagnostic system for the transfer beamline is satisfactory. The beam diagnostic components for the high-energy beamlines up to the isotope production and neutron therapy vaults and the first experimental target rooms have been installed. The high-energy slits have been delivered. The scanner and harp electronics have been installed and linked to their respective components in the beamlines. The pneumatic acuator control electronics has been manufactured, installed and is operational; provision has been made for special control features of the equipment in the therapy beamline. The high-voltage bias supply for the Faraday cups has been implemented. The installation of the beam current measurement system is nearing completion although part of it is already operational. A coaxial relay multiplexer for the capacitive phase probe signals has been manufactured and installed. The diagnostic equipment for the beamlines to isotope production and neutron therapy is thus ready for operation. 4 figs

  15. Transportation System Concept of Operations

    Energy Technology Data Exchange (ETDEWEB)

    N. Slater-Thompson

    2006-08-16

    The Nuclear Waste Policy Act of 1982 (NWPA), as amended, authorized the DOE to develop and manage a Federal system for the disposal of SNF and HLW. OCRWM was created to manage acceptance and disposal of SNF and HLW in a manner that protects public health, safety, and the environment; enhances national and energy security; and merits public confidence. This responsibility includes managing the transportation of SNF and HLW from origin sites to the Repository for disposal. The Transportation System Concept of Operations is the core high-level OCRWM document written to describe the Transportation System integrated design and present the vision, mission, and goals for Transportation System operations. By defining the functions, processes, and critical interfaces of this system early in the system development phase, programmatic risks are minimized, system costs are contained, and system operations are better managed, safer, and more secure. This document also facilitates discussions and understanding among parties responsible for the design, development, and operation of the Transportation System. Such understanding is important for the timely development of system requirements and identification of system interfaces. Information provided in the Transportation System Concept of Operations includes: the functions and key components of the Transportation System; system component interactions; flows of information within the system; the general operating sequences; and the internal and external factors affecting transportation operations. The Transportation System Concept of Operations reflects OCRWM's overall waste management system policies and mission objectives, and as such provides a description of the preferred state of system operation. The description of general Transportation System operating functions in the Transportation System Concept of Operations is the first step in the OCRWM systems engineering process, establishing the starting point for the lower

  16. Canadian pipeline transportation system : transportation assessment

    International Nuclear Information System (INIS)

    2009-07-01

    In addition to regulating the construction and operation of 70,000 km of oil and natural gas pipelines in Canada, the National Energy Board (NEB) regulates the trade of natural gas, oil and natural gas liquids. This report provided an assessment of the Canadian hydrocarbon transportation system in relation to its ability to provide a robust energy infrastructure. Data was collected from NEB-regulated pipeline companies and a range of publicly available sources to determine if adequate pipeline capacity is in place to transport products to consumers. The NEB also used throughput and capacity information received from pipeline operators as well as members of the investment community. The study examined price differentials compared with firm service tolls for transportation paths, as well as capacity utilization on pipelines and the degree of apportionment on major oil pipelines. This review indicated that in general, the Canadian pipeline transportation system continues to work effectively, with adequate pipeline capacity in place to move products to consumers who need them. 9 tabs., 30 figs., 3 appendices.

  17. Canadian hydrocarbon transportation system : transportation assessment

    International Nuclear Information System (INIS)

    2006-06-01

    This document provided an assessment of the Canadian hydrocarbon transportation system. In addition to regulating the construction and operation of Canada's 45,000 km of pipeline that cross international and provincial borders, Canada's National Energy Board (NEB) regulates the trade of natural gas, oil and natural gas liquids. The ability of pipelines to delivery this energy is critical to the country's economic prosperity. The pipeline system includes large-diameter, cross-country, high-pressure natural gas pipelines, low-pressure crude oil and oil products pipelines and small-diameter pipelines. In order to assess the hydrocarbon transportation system, staff at the NEB collected data from pipeline companies and a range of publicly available sources. The Board also held discussions with members of the investment community regarding capital markets and emerging issues. The assessment focused largely on evaluating whether Canadians benefit from an efficient energy infrastructure and markets. The safety and environmental integrity of the pipeline system was also evaluated. The current adequacy of pipeline capacity was assessed based on price differentials compared with firm service tolls for major transportation paths; capacity utilization on pipelines; and, the degree of apportionment on major oil pipelines. The NEB concluded that the Canadian hydrocarbon transportation system is working effectively, with an adequate capacity in place on existing natural gas pipelines, but with a tight capacity on oil pipelines. It was noted that shippers continue to indicate that they are reasonably satisfied with the services provided by pipeline companies and that the NEB-regulated pipeline companies are financially stable. 14 refs, 11 tabs., 28 figs., 4 appendices

  18. An ATP Binding Cassette Transporter Mediates the Uptake of α-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates

    DEFF Research Database (Denmark)

    Hansen, Morten Ejby; Fredslund, Folmer; Andersen, Joakim Mark

    2016-01-01

    that the dominant HGM commensal Bacteroides ovatus was out-competed by B. animalis subsp. lactis Bl-04 in mixed cultures growing on raffinose, the preferred ligand for the BlG16BP. By comparison, B. ovatus mono-cultures grew very efficiently on this trisaccharide. These findings suggest that the ABC-mediated uptake...... of raffinose provides an important competitive advantage, particularly against dominant Bacteroides that lack glycan-specific ABC-transporters. This novel insight highlights the role of glycan transport in defining the metabolic specialization of gut bacteria....

  19. Activation of liver X receptor decreases atherosclerosis in Ldlr⁻/⁻ mice in the absence of ATP-binding cassette transporters A1 and G1 in myeloid cells.

    Science.gov (United States)

    Kappus, Mojdeh S; Murphy, Andrew J; Abramowicz, Sandra; Ntonga, Vusisizwe; Welch, Carrie L; Tall, Alan R; Westerterp, Marit

    2014-02-01

    Liver X receptor (LXR) activators decrease atherosclerosis in mice. LXR activators (1) directly upregulate genes involved in reverse cholesterol transport and (2) exert anti-inflammatory effects mediated by transrepression of nuclear factor-κB target genes. We investigated whether myeloid cell deficiency of ATP-binding cassette transporters A1 and G1 (ABCA1/G1), principal targets of LXR that promote macrophage cholesterol efflux and initiate reverse cholesterol transport, would abolish the beneficial effects of LXR activation on atherosclerosis. LXR activator T0901317 substantially reduced inflammatory gene expression in macrophages lacking ABCA1/G1. Ldlr(-/-) mice were transplanted with Abca1(-/-)Abcg1(-/-) or wild-type bone marrow (BM) and fed a Western-type diet for 6 weeks with or without T0901317 supplementation. Abca1/g1 BM deficiency increased atherosclerotic lesion complexity and inflammatory cell infiltration into the adventitia and myocardium. T0901317 markedly decreased lesion area, complexity, and inflammatory cell infiltration in the Abca1(-/-)Abcg1(-/-) BM-transplanted mice. To investigate whether this was because of macrophage Abca1/g1 deficiency, Ldlr(-/-) mice were transplanted with LysmCreAbca1(fl/fl)Abcg1(fl/fl) or Abca1(fl/fl)Abcg1(fl/fl) BM and fed Western-type diet with or without the more specific LXR agonist GW3965 for 12 weeks. GW3965 decreased lesion size in both groups, and the decrease was more prominent in the LysmCreAbca1(fl/fl)Abcg1(fl/fl) group. The results suggest that anti-inflammatory effects of LXR activators are of key importance to their antiatherosclerotic effects in vivo independent of cholesterol efflux pathways mediated by macrophage ABCA1/G1. This has implications for the development of LXR activators that lack adverse effects on lipogenic genes while maintaining the ability to transrepress inflammatory genes.

  20. Modulation of microRNA Expression in Subjects with Metabolic Syndrome and Decrease of Cholesterol Efflux from Macrophages via microRNA-33-Mediated Attenuation of ATP-Binding Cassette Transporter A1 Expression by Statins.

    Directory of Open Access Journals (Sweden)

    Wei-Ming Chen

    Full Text Available Metabolic syndrome (MetS is a complicated health problem that encompasses a variety of metabolic disorders. In this study, we analyzed the relationship between the major biochemical parameters associated with MetS and circulating levels of microRNA (miR-33, miR-103, and miR-155. We found that miRNA-33 levels were positively correlated with levels of fasting blood glucose, glycosylated hemoglobin A1c, total cholesterol, LDL-cholesterol, and triacylglycerol, but negatively correlated with HDL-cholesterol levels. In the cellular study, miR-33 levels were increased in macrophages treated with high glucose and cholesterol-lowering drugs atorvastatin and pitavastatin. miR-33 has been reported to play an essential role in cholesterol homeostasis through ATP-binding cassette transporter A1 (ABCA1 regulation and reverse cholesterol transport. However, the molecular mechanism underlying the linkage between miR-33 and statin treatment remains unclear. In the present study, we investigated whether atorvastatin and pitavastatin exert their functions through the modulation of miR-33 and ABCA1-mediated cholesterol efflux from macrophages. The results showed that treatment of the statins up-regulated miR-33 expression, but down-regulated ABCA1 mRNA levels in RAW264.7 cells and bone marrow-derived macrophages. Statin-mediated ABCA1 regulation occurs at the post-transcriptional level through targeting of the 3'-UTR of the ABCA1 transcript by miR-33. Additionally, we found significant down-regulation of ABCA1 protein expression in macrophages treated with statins. Finally, we showed that high glucose and statin treatment significantly suppressed cholesterol efflux from macrophages. These findings have highlighted the complexity of statins, which may exert detrimental effects on metabolic abnormalities through regulation of miR-33 target genes.

  1. Modulation of microRNA Expression in Subjects with Metabolic Syndrome and Decrease of Cholesterol Efflux from Macrophages via microRNA-33-Mediated Attenuation of ATP-Binding Cassette Transporter A1 Expression by Statins.

    Science.gov (United States)

    Chen, Wei-Ming; Sheu, Wayne H-H; Tseng, Pei-Chi; Lee, Tzong-Shyuan; Lee, Wen-Jane; Chang, Pey-Jium; Chiang, An-Na

    2016-01-01

    Metabolic syndrome (MetS) is a complicated health problem that encompasses a variety of metabolic disorders. In this study, we analyzed the relationship between the major biochemical parameters associated with MetS and circulating levels of microRNA (miR)-33, miR-103, and miR-155. We found that miRNA-33 levels were positively correlated with levels of fasting blood glucose, glycosylated hemoglobin A1c, total cholesterol, LDL-cholesterol, and triacylglycerol, but negatively correlated with HDL-cholesterol levels. In the cellular study, miR-33 levels were increased in macrophages treated with high glucose and cholesterol-lowering drugs atorvastatin and pitavastatin. miR-33 has been reported to play an essential role in cholesterol homeostasis through ATP-binding cassette transporter A1 (ABCA1) regulation and reverse cholesterol transport. However, the molecular mechanism underlying the linkage between miR-33 and statin treatment remains unclear. In the present study, we investigated whether atorvastatin and pitavastatin exert their functions through the modulation of miR-33 and ABCA1-mediated cholesterol efflux from macrophages. The results showed that treatment of the statins up-regulated miR-33 expression, but down-regulated ABCA1 mRNA levels in RAW264.7 cells and bone marrow-derived macrophages. Statin-mediated ABCA1 regulation occurs at the post-transcriptional level through targeting of the 3'-UTR of the ABCA1 transcript by miR-33. Additionally, we found significant down-regulation of ABCA1 protein expression in macrophages treated with statins. Finally, we showed that high glucose and statin treatment significantly suppressed cholesterol efflux from macrophages. These findings have highlighted the complexity of statins, which may exert detrimental effects on metabolic abnormalities through regulation of miR-33 target genes.

  2. Advanced public transportation systems benefits

    Science.gov (United States)

    1996-03-01

    Benefits and cost savings for various Advanced Public Transportation Systems are outlined here. Operational efficiencies are given for Transit Management Systems in different locales, as well as compliant resolution and safety. Electronic Fare Paymen...

  3. Intelligent Transportation Systems : critical standards

    Science.gov (United States)

    1999-06-01

    Intelligent Transportation Systems (ITS) standards are industry-consensus standards that provide the details about how different systems interconnect and communicate information to deliver the ITS user services described in the National ITS Architect...

  4. Air transport system

    CERN Document Server

    Schmitt, Dieter

    2016-01-01

    The book addresses all major aspects to be considered for the design and operation of aircrafts within the entire transportation chain. It provides the basic information about the legal environment, which defines the basic requirements for aircraft design and aircraft operation. The interactions between  airport, air traffic management and the airlines are described. The market forecast methods and the aircraft development process are explained to understand the very complex and risky business of an aircraft manufacturer. The principles of flight physics as basis for aircraft design are presented and linked to the operational and legal aspects of air transport including all environmental impacts. The book is written for graduate students as well as for engineers and experts, who are working in aerospace industry, at airports or in the domain of transport and logistics.

  5. Brucella abortus mutants lacking ATP-binding cassette transporter proteins are highly attenuated in virulence and confer protective immunity against virulent B. abortus challenge in BALB/c mice.

    Science.gov (United States)

    Truong, Quang Lam; Cho, Youngjae; Park, Soyeon; Park, Bo-Kyoung; Hahn, Tae-Wook

    2016-06-01

    Brucella abortus RB51 is an attenuated vaccine strain that has been most frequently used for bovine brucellosis. Although it is known to provide good protection in cattle, it still has some drawbacks including resistance to rifampicin, residual virulence and pathogenicity in humans. Thus, there has been a continuous interest on new safe and effective bovine vaccine candidates. In the present study, we have constructed unmarked mutants by deleting singly cydD and cydC genes, which encode ATP-binding cassette transporter proteins, from the chromosome of the virulent Brucella abortus isolate from Korean cow (referred to as IVK15). Both IVK15ΔcydD and ΔcydC mutants showed increased sensitivity to metal ions, hydrogen peroxide and acidic pH, which are mimic to intracellular environment during host infection. Additionally, the mutants exhibited a significant growth defect in RAW264.7 cells and greatly attenuated in mice. Vaccination of mice with either IVK15ΔcydC or IVK15ΔcydD mutant could elicit an anti-Brucella specific immunoglobulin G (IgG) and IgG subclass responses as well as enhance the secretion of interferon-gamma, and provided better protection against challenge with B. abortus strain 2308 than with the commercial B. abortus strain RB51 vaccine. Collectively, these results suggest that both IVK15ΔcydC and IVK15ΔcydD mutants could be an attenuated vaccine candidate against B. abortus. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Thermal transport in fractal systems

    DEFF Research Database (Denmark)

    Kjems, Jørgen

    1992-01-01

    Recent experiments on the thermal transport in systems with partial fractal geometry, silica aerogels, are reviewed. The individual contributions from phonons, fractons and particle modes, respectively, have been identified and can be described by quantitative models consistent with heat capacity...

  7. Self-Organized Transport System

    Science.gov (United States)

    2009-09-28

    This report presents the findings of the simulation model for a self-organized transport system where traffic lights communicate with neighboring traffic lights and make decisions locally to adapt to traffic conditions in real time. The model is insp...

  8. Inactive trials of transport systems

    International Nuclear Information System (INIS)

    Haberlin, M.M.; Hardy, A.R.

    1985-06-01

    The design and manufacture of a mock-up of a crate handling and size reduction (CHSR) facility, an experimental programme on the evaluation of a commercial air-transporter, and the selection, manufacture and commissioning trials of an integrated conveyor system for transporting crated waste into and within the mock-up facility, are considered. The mock-up facility was used for the test programme on the air-transporter and conveyor system. The air-transporter was considered suitable for transporting waste on the metal floor in the main dismantling area of the CHSR facility because it can tolerate asymmetric loading, the exhaust air flow liberated from the air-pads is low and it has excellent manoeuvrability. Commissioning trials were carried out on a commercial conveyor system consisting of unpowered rollers in the reception area, a powered slatted conveyor in the air-lock and an unpowered roller table placed on the air-transporter in the working area. It was demonstrated that a large asymmetrically loaded wooden crate can be transported into and within the facility by this method. Further design and experimental work necessary before the system can be used for remote operation is discussed. (author)

  9. ANALYISIS OF TRANSPORTATION SYSTEMS AND TRANSPORTATION POLICIES IN TURKEY

    Directory of Open Access Journals (Sweden)

    Ali Payıdar AKGÜNGÖR

    2004-03-01

    Full Text Available Transportation systems have to be considered and analysed as a whole while transportation demand, becoming as a natural outcome of socioeconomic and socio-cultural structure, is being evaluated. It is desired that transportation system, which will be selected for both passenger and freight transport, should be rapid, economic, safe, causing least harm to environment and appropriate for the conditions of a country. However, it is difficult for a transportation system to have all these properties. Every transportation system has advantages and disadvantages over each other. Therefore, comprehensive plans for future periods have to be prepared and how the sources of the country should be reasonably distributed among transportation systems must be investigated. Also, transportation plans have to be prepared to get coordinated operations among transportation systems while great investments are instituted in the entire country. There is no doubt that it is possible with combined transportation instead of concentration on one transportation system. Transportation policies in Turkey should be questioned since the level of highway transportation usage reaches to 95 % and level of sea transportation usage drops to less than 1 % in spite of being surrounded with sea in three sides of our land. In this paper, transportation systems and transportation policies in Turkey are evaluated in general and problems are analysed. Proposals are presented for the solutions of these problems.

  10. ANALYISIS OF TRANSPORTATION SYSTEMS AND TRANSPORTATION POLICIES IN TURKEY

    OpenAIRE

    Ali Payıdar AKGÜNGÖR; Abdulmuttalip DEMİREL

    2004-01-01

    Transportation systems have to be considered and analysed as a whole while transportation demand, becoming as a natural outcome of socioeconomic and socio-cultural structure, is being evaluated. It is desired that transportation system, which will be selected for both passenger and freight transport, should be rapid, economic, safe, causing least harm to environment and appropriate for the conditions of a country. However, it is difficult for a transportation system to have all these properti...

  11. Advanced Transport Operating Systems Program

    Science.gov (United States)

    White, John J.

    1990-01-01

    NASA-Langley's Advanced Transport Operating Systems Program employs a heavily instrumented, B 737-100 as its Transport Systems Research Vehicle (TRSV). The TRSV has been used during the demonstration trials of the Time Reference Scanning Beam Microwave Landing System (TRSB MLS), the '4D flight-management' concept, ATC data links, and airborne windshear sensors. The credibility obtainable from successful flight test experiments is often a critical factor in the granting of substantial commitments for commercial implementation by the FAA and industry. In the case of the TRSB MLS, flight test demonstrations were decisive to its selection as the standard landing system by the ICAO.

  12. The transport system approval concept

    International Nuclear Information System (INIS)

    Pettersson, B.G.

    1991-01-01

    The needs for, and merits of, a new concept for the safety assessment and approval of shipments of radioactive materials is introduced and discussed. The purpose of the new concept is to enable and encourage integration of analysis and review of transport safety with similar safety analysis and review of the handling operations involving the radioactive material at the despatching and receiving ends of a shipment. Safety contributing elements or functions of the means of transport (the Transport System) can thus readily be taken into account in the assessment. The objective is to avoid constraints -experienced or potential - introduced by the package functional provisions contained in the transport regulations, whilst maintaining safety during transport, as well as during facility handling operations, at least at the level at the level currently established. (author)

  13. Bevalac beam transport system

    International Nuclear Information System (INIS)

    Avery, R.; Behrsing, G.; Morgado, R.; Rondeau, D.; Salsig, W.; Selph, F.; Staples, J.; Yourd, R.

    1975-03-01

    The Bevalac consists of, in part, a 200 meter long transfer line between the SuperHILAC and the Bevatron, which are at differing elevation. Unique features in the construction of the transfer line are described. The line, located largely outside, must cope with a natural environment. Part of the line passes through a hillside, requiring some unique support and alignment techniques. The dipoles are of the tape-wound variety and the steering magnets use printed circuit conductors. The vacuum system and an inexpensive and effective destructive monitoring system are described. (U.S.)

  14. Transport phenomena in particulate systems

    CERN Document Server

    Freire, José Teixeira; Ferreira, Maria do Carmo

    2012-01-01

    This volume spans 10 chapters covering different aspects of transport phenomena including fixed and fluidized systems, spouted beds, electrochemical and wastewater treatment reactors. This e-book will be valuable for students, engineers and researchers aiming to keep updated on the latest developments on particulate systems.

  15. NUHOMS transportation system interfaces

    International Nuclear Information System (INIS)

    McConaghy, W.J.; Lehnert, R.A.; Rasmussen, R.W.

    1989-01-01

    The NUHOMS system utilizes a reinforced concrete Horizontal Storage Module (HSM) to store spent nuclear fuel assemblies which are sealed in a Dry Shielded Canister (DSC). The DSC has an internal basket assembly designed to hold 24 PWR or 60 BWR spent fuel assemblies. The HSMs are constructed in interconnected arrays on the utilities reactor site with each HSM holding one DSC. The HSMs and DSCs are the principal components of the Independent Spent Fuel Storage Installation (ISFSI) for which plants are granted a 10CFR72 (1) license by the US Nuclear Regulatory Commission (NRC) for interim dry storage. A complete description of the NUHOMS system for dry storage of spent fuel and its operation is contained in the NRC approved NUHOMS Topical Report and related publications (2, 3, 4, 6)

  16. Intelligent Transport Systems in the Management of Road Transportation

    Science.gov (United States)

    Kalupová, Blanka; Hlavoň, Ivan

    2016-11-01

    Extension of European Union causes increase of free transfer of people and goods. At the same time they raised the problems associated with the transport, e.g. congestion and related accidents on roads, air traffic delays and more. To increase the efficiency and safety of transport, the European Commission supports the introduction of intelligent transport systems and services in all transport sectors. Implementation of intelligent transport systems and services in the road transport reduces accident frequency, increases the capacity of existing infrastructure and reduces congestions. Use of toll systems provides resources needed for the construction and operation of a new road network, improves public transport, cycling transport and walking transport, and also their multimodal integration with individual car transport.

  17. The ITER divertor cassette project meeting

    International Nuclear Information System (INIS)

    Merola, M.; Riccardi, B.; Tivey, R.

    1999-01-01

    The Divertor Cassette Project topical meeting was held on May 26-28, 1999 at the ENEA Brasimone Research Centre in Camugnano (Bologna), Italy. Specialists from all the four Parties and the JCT participated in the meeting. It was concluded that the Divertor Cassette Project has significantly contributed to solving a large part of the critical issues of the ITER divertor design

  18. On design and development of additional End-Effectors for the Cassette Multifunctional Mover

    Energy Technology Data Exchange (ETDEWEB)

    Valkama, Peetu, E-mail: peetu.valkama@tut.fi [Department of Intelligent Hydraulics and Automation, Tampere University of Technology, P.O. Box 589, FI-33720 Tampere (Finland); Mattila, J.; Amjad, F.; Vaeyrynen, J.; Vilenius, M. [Department of Intelligent Hydraulics and Automation, Tampere University of Technology, P.O. Box 589, FI-33720 Tampere (Finland); Siuko, M. [VTT Technical Research Centre of Finland, P.O. Box 1300, FI-33101 Tampere (Finland); Semeraro, L.; Esque, S. [F4E, Fusion for Energy, Torres Diagonal Litoral B3, Josep Pla 2, 08019 Barcelona (Spain)

    2011-10-15

    The divertor area of ITER Vacuum Vessel (VV) consists of 54 modular cassettes which must be replaced three times during the estimated 20 years of operation of the ITER. Cassette Multifunctional Mover (CMM) and Cassette Toroidal Mover (CTM) are used in the cassette remote handling (RH). In this paper we discuss the design and development process for the RH equipment to be used in the ITER environment. Design concepts for the Standard Cassette End-Effector and Central Cassette End-Effector are described and the conceptual design phase methodology is presented. The main improvements of the new End-Effector concept designs are more robust and reliable assembly process with reduced CMM mover assembly accuracy requirement. New Central Cassette locking system was developed to address the high forces and contact pressures emerging during the Central Cassette installation. The chosen design concepts are verified with virtual reality simulations and are fulfilling the requirements defined in the concept design phase, including structural, assembly sequence, safety and reliability.

  19. Expression and regulation of prostaglandin transporters, ATP-binding cassette, subfamily C, member 1 and 9, and solute carrier organic anion transporter family, member 2A1 and 5A1 in the uterine endometrium during the estrous cycle and pregnancy in pigs

    Directory of Open Access Journals (Sweden)

    Hwanhee Jang

    2017-05-01

    Full Text Available Objective Prostaglandins (PGs function in various reproductive processes, including luteolysis, maternal pregnancy recognition, conceptus development, and parturition. Our earlier study has shown that PG transporters ATP-binding cassette, subfamily C, member 4 (ABCC4 and solute carrier organic anion transporter family, member 2A1 (SLCO2A1 are expressed in the uterine endometrium in pigs. Since several other PG transporters such as ABCC1, ABCC9, SLCO4C1, and SLCO5A1 are known to be present in the uterine endometrium, this study investigated the expression of these PG transporters in the porcine uterine endometrium and placenta. Methods Uterine endometrial tissues were obtained from gilts on day (D 12 and D15 of the estrous cycle and days 12, 15, 30, 60, 90, and 114 of pregnancy. Results ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNAs were expressed in the uterine endometrium, and levels of expression changed during the estrous cycle and pregnancy. Expression of ABCC1 and ABCC9 mRNAs was localized mainly to luminal and glandular epithelial cells in the uterine endometrium, and chorionic epithelial cells during pregnancy. Conceptuses during early pregnancy and chorioallantoic tissues from mid to late pregnancy also expressed these PG transporters. Estradiol-17β increased the expression of ABCC1 and SLCO5A1, but not ABCC9 and SLCO4C1 mRNAs and increasing doses of interleukin-1β induced the expression of ABCC9, SLCO4C1, and SLCO5A1 mRNAs in endometrial explant tissues. Conclusion These data showed that several PG transporters such as ABCC1, ABCC9, SLCO4C1, and SLCO5A1 were expressed at the maternal-conceptus interface, suggesting that these PG transporters may play an important role in the establishment and maintenance of pregnancy by regulating PG transport in the uterine endometrium and placenta in pigs.

  20. Surveillance systems for intermodal transportation

    Science.gov (United States)

    Jakovlev, Sergej; Voznak, Miroslav; Andziulis, Arunas

    2015-05-01

    Intermodal container monitoring is considered a major security issue in many major logistic companies and countries worldwide. Current representation of the problem, we face today, originated in 2002, right after the 9/11 attacks. Then, a new worldwide Container Security Initiative (CSI, 2002) was considered that shaped the perception of the transportation operations. Now more than 80 larger ports all over the world contribute to its further development and integration into everyday transportation operations and improve the regulations for the developing regions. Although, these new improvements allow us to feel safer and secure, constant management of transportation operations has become a very difficult problem for conventional data analysis methods and information systems. The paper deals with a proposal of a whole new concept for the improvement of the Containers Security Initiative (CSI) by virtually connecting safety, security processes and systems. A conceptual middleware approach with deployable intelligent agent modules is proposed to be used with possible scenarios and a testbed is used to test the solution. Middleware examples are visually programmed using National Instruments LabView software packages and Wireless sensor network hardware modules. An experimental software is used to evaluate he solution. This research is a contribution to the intermodal transportation and is intended to be used as a means or the development of intelligent transport systems.

  1. Quantum transport in complex system

    International Nuclear Information System (INIS)

    Kusnezov, D.; Bulgac, A.; DoDang, G.

    1998-01-01

    We derive the influence function and the effective dynamics of a quantum systems coupled to a chaotic environment, using very general parametric and banded random matrices to describe the quantum properties of a chaotic bath. We find that only in certain limits the thermalization can result from the environment. We study the general transport problems including escape, fusion and tunneling (fission). (author)

  2. Optimal concentrations in transport systems

    Science.gov (United States)

    Jensen, Kaare H.; Kim, Wonjung; Holbrook, N. Michele; Bush, John W. M.

    2013-01-01

    Many biological and man-made systems rely on transport systems for the distribution of material, for example matter and energy. Material transfer in these systems is determined by the flow rate and the concentration of material. While the most concentrated solutions offer the greatest potential in terms of material transfer, impedance typically increases with concentration, thus making them the most difficult to transport. We develop a general framework for describing systems for which impedance increases with concentration, and consider material flow in four different natural systems: blood flow in vertebrates, sugar transport in vascular plants and two modes of nectar drinking in birds and insects. The model provides a simple method for determining the optimum concentration copt in these systems. The model further suggests that the impedance at the optimum concentration μopt may be expressed in terms of the impedance of the pure (c = 0) carrier medium μ0 as μopt∼2αμ0, where the power α is prescribed by the specific flow constraints, for example constant pressure for blood flow (α = 1) or constant work rate for certain nectar-drinking insects (α = 6). Comparing the model predictions with experimental data from more than 100 animal and plant species, we find that the simple model rationalizes the observed concentrations and impedances. The model provides a universal framework for studying flows impeded by concentration, and yields insight into optimization in engineered systems, such as traffic flow. PMID:23594815

  3. Neoclassical transport in toroidal systems

    International Nuclear Information System (INIS)

    Wobig, H.

    1992-01-01

    The neoclassical theory of general toroidal equilibria is reformulated. The toroidal equilibrium of tokamaks and stellarators are described in Hamada coordinates. The relevant geometrical parameters are identified and it is shown how the reduction of Pfirsch-Schluter currents affects neoclassical transport and bootstrap effects. General flux-friction relations between thermodynamic forces and fluxes are derived. In drift-kinetic approximation the neoclassical transport coefficients are Onsager symmetric. Since a toroidal loop voltage is included, the theory is valid for all toroidal systems. (Author)

  4. Integrated Intermodal Passenger Transportation System

    Science.gov (United States)

    Klock, Ryan; Owens, David; Schwartz, Henry; Plencner, Robert

    2012-01-01

    Modern transportation consists of many unique modes of travel. Each of these modes and their respective industries has evolved independently over time, forming a largely incoherent and inefficient overall transportation system. Travelers today are forced to spend unnecessary time and efforts planning a trip through varying modes of travel each with their own scheduling, pricing, and services; causing many travelers to simply rely on their relatively inefficient and expensive personal automobile. This paper presents a demonstration program system to not only collect and format many different sources of trip planning information, but also combine these independent modes of travel in order to form optimal routes and itineraries of travel. The results of this system show a mean decrease in inter-city travel time of 10 percent and a 25 percent reduction in carbon dioxide emissions over personal automobiles. Additionally, a 55 percent reduction in carbon dioxide emissions is observed for intra-city travel. A conclusion is that current resources are available, if somewhat hidden, to drastically improve point to point transportation in terms of time spent traveling, the cost of travel, and the ecological impact of a trip. Finally, future concepts are considered which could dramatically improve the interoperability and efficiency of the transportation infrastructure.

  5. System Convergence in Transport Modelling

    DEFF Research Database (Denmark)

    Rich, Jeppe; Nielsen, Otto Anker; Cantarella, Guilio E.

    2010-01-01

    A fundamental premise of most applied transport models is the existence and uniqueness of an equilibrium solution that balances demand x(t) and supply t(x). The demand consists of the people that travel in the transport system and on the defined network, whereas the supply consists of the resulting...... level-of-service attributes (e.g., travel time and cost) offered to travellers. An important source of complexity is the congestion, which causes increasing demand to affect travel time in a non-linear way. Transport models most often involve separate models for traffic assignment and demand modelling...... iterating between a route-choice (demand) model and a time-flow (supply) model. It is generally recognised that a simple iteration scheme where the level-of-service level is fed directly to the route-choice and vice versa may exhibit an unstable pattern and lead to cyclic unstable solutions. It can be shown...

  6. [The reduction of the radiation dosage by means of storage phosphor-film radiography compared to a conventional film-screen system with a grid cassette on a skull phantom].

    Science.gov (United States)

    Heyne, J P; Merbold, H; Sehner, J; Neumann, R; Freesmeyer, M; Jonetz-Mentzel, L; Kaiser, W A

    1999-07-01

    How much can the radiation dose be reduced for skull radiography by using digital luminescence radiography (DLR) compared to a conventional screen film system with a grid cassette? A skull phantom (3M) was x-rayed in anterior-posterior orientation using both a conventional screen film system with grid cassette and DLR (ADC-70, Agfa). The tube current time product (mAs) was diminished gradually while keeping the voltage constant. The surface entrance dose was measured by a sensor of Dosimax (Wellhöfer). Five investigators evaluated the images by characteristic and critical features, spatial resolution and contrast. The surface entrance dose at 73 kV/22 mAs was 0.432 mGy in conventional screen film system and 0.435 mGy in DLR. The images could be evaluated very well down to an average dose of 71% (0.308 mGy; SD 0.050); sufficient images were obtained down to an average dose of 31% (0.136 mGy; SD 0.065). The resolution of the line pairs were reduced down to 2 levels depending on the investigator. Contrast was assessed as being very good to sufficient. The acceptance of the postprocessed images (MUSICA-software) was individually different and resulted in an improvement of the assessment of bone structures and contrast in higher dose ranges only. For the sufficient assessment of a possible fracture/of paranasal sinuses/of measurement of the skull the dose can be reduced to at least 56% (phi 31%; SD 14.9%)/40% (phi 27%; SD 9.3%)/18% (phi 14%; SD 4.4%). Digital radiography allows question-referred exposure parameters with clearly reduced dose, so e.g. for fracture exclusion 73 kV/12.5 mAs and to skull measurement 73 kV/4 mAs.

  7. The economic potential of a cassette-type-reactor-installed nuclear ice-breaking container ship

    International Nuclear Information System (INIS)

    Kondo, K.; Takamasa, T.

    2000-01-01

    The design concept of the cassette-type-reactor MRX (Marine Reactor X), being under development in Japan for the nuclear ice-breaker container ship is described. The MRX reactor is the monoblock water-cooled and moderated reactor with passive cooling system of natural circulation. It is shown that application of the reactor being under consideration gives an opportunity to decrease greatly the difference in prices for similar nuclear and diesel ships. Economic estimations for applicability of the nuclear ice-breaker container ship with the MRX reactor in Arctics for transportation of standard containers TEU from Europe to Far East as compared with transportation of the same containers by diesel ships via Suets Canal are made [ru

  8. Thermal comfort and indoor air quality in the lecture room with 4-way cassette air-conditioner and mixing ventilation system

    International Nuclear Information System (INIS)

    Noh, Kwang-Chul; Jang, Jae-Soo; Oh, Myung-Do

    2007-01-01

    We performed the experimental and the numerical studies on thermal comfort (TC) and indoor air quality (IAQ) in the lecture room with cooling loads when the operating conditions are changed. Predicted mean vote (PMV) value and CO 2 concentration of the lecture room were measured and compared to the numerical results. Both of them showed a reasonable agreement with each other and then we applied the numerical model to analyze TC and IAQ for a couple of different operating conditions. From the results we found that the increment of the discharge angle of 4-way cassette air-conditioner makes uniformity of TC worse, but rarely affects IAQ. It turned out that TC and IAQ are hardly affected by the variation of the discharge airflow. Finally TC was merely affected by the increment of the ventilation rate, but when the ventilation rate is more than 800m 3 /h, the average CO 2 concentration can be satisfied with the standard limits of Japanese in our case studies. (author)

  9. Cassette for handling banknotes or the like

    Science.gov (United States)

    Lundblad, Leif

    1981-08-11

    A cassette for banknotes and like valuable articles is provided with a displaceable lid (6) and locking means (10) for latching the lid of the cassette when the cassette is located outside a housing (25) in which it is intended to be placed. An operating means (8) is arranged to co-act with the locking means and with a latching element (15). The latching element is arranged to be released in dependence upon a pre-set program. A signal circuit is arranged to send a code signal to a detector circuit (23) when electrical contact elements on the cassette and the housing co-act with one another, which detector circuit, when the signal coincides with the signal program in the detector circuit, causes a signal to be sent for moving the latching means to a non-latching position.

  10. Design of SMART steam generator cassette

    International Nuclear Information System (INIS)

    Kim, Y. W.; Kim, J. I.; Jang, M. H.

    2001-01-01

    Basic design development for the steam generator to be installed in the integral reactor SMART has been performed. Optimization of the steam generator shape, determination of the basic dimension and confirmation of the structural strength have been carried out. Individual steam generator cassette can be replaced in the optimized design concept of steam generator. Shape design of the steam generator cassette has been done on the computer based on 3-D CAE strategy. The structural integrity of the developed steam generator was investigated by performing the dynamic analysis for the steam generator cassette, flow induced vibration analysis for the tube bundle, and the thermo-mechanical analysis for the module header and tube. As for the manufacturing of steam generator, the numerical and the experimental simulation have been carried to control the amount of spring back and to eliminate residual stress. SMART steam generator cassette was developed by a sequential research of the aforementioned activities

  11. Physical System Requirements: Transport Waste

    International Nuclear Information System (INIS)

    1992-04-01

    The Nuclear Waste Policy Act (NWPA) of 1982 assigned to the Department of Energy (DOE) the responsibility for managing the disposal of spent nuclear fuel and high-level radioactive waste and established the Office of Civilian Radioactive Waste Management (OCRWM) for that purpose. The Secretary of Energy, in his November 1989 report to Congress (DOE/RW-0247), announced three new initiatives for the conduct of the Civilian Radioactive Waste Management (CRWM) program. One of these initiatives was to establish improved management structure and procedures. In response, OCRWM performed a management study and the Director subsequently issued the Management Systems Improvement Strategy (MSIS) on August 10, 1990, calling for a rigorous implementation of systems engineering principles with a special emphasis on functional analysis. The functional analysis approach establishes a framework for integrating the program management efforts with the technical requirements analysis into a single, unified, and consistent program. This approach recognizes that just as the facilities and equipment comprising the physical waste management system must perform certain functions, so must certain programmatic and management functions be performed within the program in order to successfully bring the physical system into being. The objective of this document is to establish the essential functions, requirements, interfaces, and system architecture for the Transport Waste mission. Based upon the Nuclear Waste Policy Act, the mission of the Waste Transportation System is to transport SNF and/or HLW from the purchaser's/producer's facilities to, and between, NWMS facilities in a manner that protects the health and safety of the public and of workers and the quality of the environment makes effective use of financial and other resources, and to the fullest extent possible uses the private sector

  12. sphingosine-1-phosphate transport and its role in immunology

    NARCIS (Netherlands)

    Reitsema, V.; Bouma, Hjalmar; Kok, Jan

    2014-01-01

    Sphingosine-1-phosphate (S1P) is a sphingolipid metabolite with many important functions in cellular and systemic physiology, including the immune system. As it cannot traverse the membrane, it is exported from cells by transporters. Several members of the ATP-binding cassette (ABC) transporter

  13. Radiation exposure reduction by use of Kevlar cassettes in the neonatal nursery

    International Nuclear Information System (INIS)

    Herman, M.W.; Mak, H.K.; Lachman, R.S.

    1987-01-01

    A study was performed to determine whether the use of Kevlar cassettes in the neonatal intensive care nursery would reduce radiation exposure to patients. The radiation dose to the neonates was measured by using thermoluminescent dosimeters. In addition, the attenuation of the Kevlar cassettes and the sensitivity of the film-screen combination were compared with the previously used system. The greatest radiation reduction using a mobile X-ray unit was 27%; based on sensitivity measurements, the theoretical reduction averaged 38%. The reduction in radiation exposure resulted from reduced attenuation by the Kevlar cassette

  14. Effectiveness of work zone intelligent transportation systems.

    Science.gov (United States)

    2013-12-01

    In the last decade, Intelligent Transportation Systems (ITS) have increasingly been deployed in work zones by state departments of transportation. Also known as smart work zone systems they improve traffic operations and safety by providing real-time...

  15. ADVANCES IN ZERO ENERGY TRANSPORTATION SYSTEMS

    OpenAIRE

    Ahmad, Othman

    2017-01-01

    Hyperloop mass transportation systems are activelydeveloped at the moment. They represent the forefront development of the ZeroEnergy Transportation systems where air drag is minimized by travelling in avacuum and friction is reduced by non-contact bearings. Hyperloop supportersare confident that the cost of their transportation systems would be lowcompared to existing transportation systems because of the low loss andtherefore low energy consumption as well as other cost-saving techniquesdoc...

  16. Reduction of radiation dose by using digital luminescence radiography compared to conventional screen film system with grid cassette; Reduktion der Strahlendosis mittels Speicherfolienradiographie im Vergleich zum konventionellen Film-Folien-System mit Rasterkassette am Schaedelphantom

    Energy Technology Data Exchange (ETDEWEB)

    Heyne, J.P.; Merbold, H.; Neumann, R.; Freesmeyer, M.; Jonetz-Mentzel, L.; Kaiser, W.A. [Friedrich-Schiller-Univ. Jena, Inst. fuer Diagnostische und Interventionelle Radiologie (Germany); Sehner, J. [AGFA-Deutschland, Vertriebsgesellschaft (Germany)

    1999-07-01

    Purpose: How much can the radiation dose be reduced for skull radiography by using digital luminescence radiography (DLR) compared to a conventional screen film system with a grid cassette? Methods and Materials: A skull phantom (3M) was X-rayed in anterior-posterior orientation using both a conventional screen film system with grid cassette and DLR (ADC-70, Agfa). The tube current time product (mAs) was diminished gradually while keeping the voltage constant. The surface entrance dose was measured by a sensor of Dosimax (Wellhoefer). Five investigators evaluated the images by characteristic and critical features, spatial resolution and contrast. Results: The surface entrance dose at 73 kV/22 mAs was 0,432 mGy in conventional screen film system and 0,435 mGy in DLR. The images could be evaluated very well down to an average dose of 71% (0,308 mGy; SD 0,050); sufficient images were obtained down to an average dose of 31% (0,136 mGy; SD 0,065). The resolution of the line pairs were reduced down to a 2 levels depending on the investigator. Contrast was assessed as being very good to sufficient. The acceptance of the postprocessed images (MUSICA-software) was individually different and resultde in an improvement of the assessment of bone structures an contrast in higher dose ranges only. Conclusion: For the sufficient assessment of a possible fracture/of paranasal sinuses/of measurement the skull the dose can be reduced to at least 56% (31%; SD 14,9%)/40% (27%; SD 9,3%)/18% (14%; SD 4,4%). Digital radiography allows question-referred exposure parameters with clearly reduced dose, so e.g. for fracture exclusion 73 kV/12,5 mAs and to skull measurement 73 kV/4 mAs. (orig.) [Deutsch] Ziel: Wie weit kann unter Einsatz der rasterlosen Speicherfolienradiographie bei einer Schaedelaufnahme die Strahlendosis im Vergleich zum Film-Folien-System (FFS) mit Rasterkassette (RK) fragestellungsbezogen gesenkt werden? Material und Methode: Ein Schaedelphantom (3M) wurde konventionell

  17. Road Transportable Analytical Laboratory system

    International Nuclear Information System (INIS)

    Finger, S.M.; Keith, V.F.; Spertzel, R.O.; De Avila, J.C.; O'Donnell, M.; Vann, R.L.

    1993-09-01

    This developmental effort clearly shows that a Road Transportable Analytical Laboratory System is a worthwhile and achievable goal. The RTAL is designed to fully analyze (radioanalytes, and organic and inorganic chemical analytes) 20 samples per day at the highest levels of quality assurance and quality control. It dramatically reduces the turnaround time for environmental sample analysis from 45 days (at a central commercial laboratory) to 1 day. At the same time each RTAL system will save the DOE over $12 million per year in sample analysis costs compared to the costs at a central commercial laboratory. If RTAL systems were used at the eight largest DOE facilities (at Hanford, Savannah River, Fernald, Oak Ridge, Idaho, Rocky Flats, Los Alamos, and the Nevada Test Site), the annual savings would be $96,589,000. The DOE's internal study of sample analysis needs projects 130,000 environmental samples requiring analysis in FY 1994, clearly supporting the need for the RTAL system. The cost and time savings achievable with the RTAL system will accelerate and improve the efficiency of cleanup and remediation operations throughout the DOE complex

  18. Urban Transportation Planning Short Course: Evaluation of Alternative Transportation Systems.

    Science.gov (United States)

    Federal Highway Administration (DOT), Washington, DC.

    This urban transportation pamphlet delves into the roles of policy groups and technical staffs in evaluating alternative transportation plans, evaluation criteria, systems to evaluate, and evaluation procedures. The introduction admits the importance of subjective, but informed, judgment as an effective tool in weighing alternative transportation…

  19. Performances of the HL (Hyperloop) transport system

    NARCIS (Netherlands)

    van Goeverden, C.D.; Milakis, D.; Janic, M.; Konings, J.W.; Cools, M.; Limbourg, S.

    2017-01-01

    This paper deals with an analysis of performances of the HL (Hyperloop) transport system considered as an advanced transport alternative to the existing APT (Air Passenger Transport) and HSR (High Speed Rail) systems. The considered performances are operational, financial, social and environmental.

  20. Radioisotope Thermoelectric Generator Transport Trailer System

    International Nuclear Information System (INIS)

    Ard, K.E.; King, D.A.; Leigh, H.; Satoh, J.A.

    1994-01-01

    The Radioisotope Thermoelectric Generator (RTG) Transportation System, designated as System 100, comprises four major systems. The four major systems are designated as the Packaging System (System 120), Trailer System (System 140), Operations and Ancillary Equipment System system 160), and Shipping and Receiving Facility Transport System (System 180). Packaging System (System 120), including the RTG packaging is licensed (regulatory) hardware; it is certified by the US Department of Energy to be in accordance with Title 10, Code of federal Regulations, Part 71 (10 CFR 71). System 140, System 160, and System 180 are nonlicensed (nonregulatory) hardware

  1. SDDOT transportation systems management & operations program plan.

    Science.gov (United States)

    2016-06-01

    The objective of this project is the development of a comprehensive Transportation Systems Management and : Operations (TSM&O) Program Plan for the South Dakota Department of Transportation. This plan guides : business planning and strategic decision...

  2. Anomalous transport in mirror systems

    International Nuclear Information System (INIS)

    Post, R.F.

    1979-01-01

    As now being explored for fusion applications confinement systems based on the mirror principle embody two kinds of plasma regimes. These two regimes are: (a) high-beta plasmas, stabilized against MHD and other low frequency plasma instabilities by magnetic-well fields, but characterized by non-Maxwellian ion distributions; (b) near-Maxwellian plasmas, confined electrostatically (as in the tandem mirror) or in a field-reversed region within the mirror cell. Common to both situations are the questions of anomalous transport owing to high frequency instabilities in the non-maxwellian portions of the plasmas. This report will summarize the status of theory and of experimental data bearing on these questions, with particular reference to the high temperature regimes of interest for fusion power

  3. Transport barrier in Helical system

    International Nuclear Information System (INIS)

    Ida, Katsumi

    1998-01-01

    Experiments on the transport barrier in Helical plasmas are reviewed. There are two mechanisms of transport improvement, that results in the formation of the transport barrier. One is the improvement of neoclassical transport by reducing the ripple loss with radial electric field, which exist only in helical plasma. The other is the improvement of anomalous transport due to the suppression of fluctuations associated with a radial electric field shear both in tokamak and helical plasma. The formation of the transport barrier can be triggered by the radial electric field shear associated with the transition of the radial electric field (L/H transition or ion-electron root transition) or the peaked density or the optimization of magnetic field shear. The mechanisms of transport barrier formation are also discussed. (author). 60 refs

  4. Intelligent transportation systems problems and perspectives

    CERN Document Server

    Pamuła, Wiesław

    2016-01-01

    This book presents a discussion of problems encountered in the deployment of Intelligent Transport Systems (ITS). It puts emphasis on the early tasks of designing and proofing the concept of integration of technologies in Intelligent Transport Systems. In its first part the book concentrates on the design problems of urban ITS. The second part of the book features case studies representative for the different modes of transport. These are freight transport, rail transport and aerospace transport encompassing also space stations. The book provides ideas for deployment which may be developed by scientists and engineers engaged in the design of Intelligent Transport Systems. It can also be used in the training of specialists, students and post-graduate students in universities and transport high schools.    .

  5. Evaluation of intelligent transport systems impact on school transport safety

    Directory of Open Access Journals (Sweden)

    Jankowska-Karpa Dagmara

    2017-01-01

    Full Text Available The integrated system of safe transport of children to school using Intelligent Transport Systems was developed and implemented in four locations across Europe under the Safeway2School (SW2S project, funded by the EU. The SW2S system evaluation included speed measurements and an eye-tracking experiment carried out among drivers who used the school bus route, where selected elements of the system were tested. The subject of the evaluation were the following system elements: pedestrian safety system at the bus stop (Intelligent Bus Stop and tags for children, Driver Support System, applications for parents’ and students’ mobile phones, bus stop inventory tool and data server. A new sign designed for buses and bus stops to inform about child transportation/children waiting at the bus stop was added to the system. Training schemes for system users were also provided. The article presents evaluation results of the impact of selected elements of the SW2S system on school transport safety in Poland.

  6. ACID: annotation of cassette and integron data

    Directory of Open Access Journals (Sweden)

    Stokes Harold W

    2009-04-01

    Full Text Available Abstract Background Although integrons and their associated gene cassettes are present in ~10% of bacteria and can represent up to 3% of the genome in which they are found, very few have been properly identified and annotated in public databases. These genetic elements have been overlooked in comparison to other vectors that facilitate lateral gene transfer between microorganisms. Description By automating the identification of integron integrase genes and of the non-coding cassette-associated attC recombination sites, we were able to assemble a database containing all publicly available sequence information regarding these genetic elements. Specialists manually curated the database and this information was used to improve the automated detection and annotation of integrons and their encoded gene cassettes. ACID (annotation of cassette and integron data can be searched using a range of queries and the data can be downloaded in a number of formats. Users can readily annotate their own data and integrate it into ACID using the tools provided. Conclusion ACID is a community resource providing easy access to annotations of integrons and making tools available to detect them in novel sequence data. ACID also hosts a forum to prompt integron-related discussion, which can hopefully lead to a more universal definition of this genetic element.

  7. The ITER Divertor Cassette Project meeting

    International Nuclear Information System (INIS)

    Akiba, M.; Tivey, R.

    2000-01-01

    The Divertor Cassette Project topical meeting took place on April 5-7, 2000 at the JAERI Naka site in Japan. The meeting focused on the progress made by the three parties under task agreements on the development of carbon-fibre composite and tungsten armored high flux plasma-facing components

  8. Nonlinear transport of dynamic system phase space

    International Nuclear Information System (INIS)

    Xie Xi; Xia Jiawen

    1993-01-01

    The inverse transform of any order solution of the differential equation of general nonlinear dynamic systems is derived, realizing theoretically the nonlinear transport for the phase space of nonlinear dynamic systems. The result is applicable to general nonlinear dynamic systems, with the transport of accelerator beam phase space as a typical example

  9. Magnetic levitation -The future transport system

    International Nuclear Information System (INIS)

    Rairan, Danilo

    2000-01-01

    The paper made a recount of the main advantages and disadvantages of the traditional systems of transport with base in electric power and it shows as the systems that use the magnetic levitation they are the future of the transport. Additionally it presents the physical principle of operation of the two main systems developed at the present time

  10. Integrated transport and renewable energy systems

    DEFF Research Database (Denmark)

    Mathiesen, Brian Vad; Lund, Henrik; Nørgaard, P.

    2008-01-01

    No single technology can solve the problem of ever increasing CO2 emissions from transport. Here, a coherent effort to integrate transport into energyplanning is proposed, using multiple means promoting sustainable transport. It is concluded that a 100 per cent renewable energy transport system...... is possible but is connected to significant challenges in the path towards it. Biomass is a limited resource and it is important to avoid effecting the production of food. The integration of the transport with the energy system is crucial as is a multi-pronged strategy. Short term solutions have to consider...

  11. A National MagLev Transportation System

    Science.gov (United States)

    Wright, Michael R.

    2003-01-01

    The case for a national high-speed magnetic-levitation (MagLev) transportation system is presented. Focus is on current issues facing the country, such as national security, the economy, transportation, technology, and the environment. NASA s research into MagLev technology for launch assist is also highlighted. Further, current socio-cultural norms regarding motor-vehicle-based transportation systems are questioned in light of the problems currently facing the U.S. The multidisciplinary benefits of a long-distance MagLev system support the idea that such a system would be an important element of a truly multimodal U.S. transportation infrastructure.

  12. METHODS OF INTEGRATED OPTIMIZATION MAGLEV TRANSPORT SYSTEMS

    Directory of Open Access Journals (Sweden)

    A. Lasher

    2013-09-01

    Full Text Available Purpose. To demonstrate feasibility of the proposed integrated optimization of various MTS parameters to reduce capital investments as well as decrease any operational and maintenance expense. This will make use of MTS reasonable. At present, the Maglev Transport Systems (MTS for High-Speed Ground Transportation (HSGT almost do not apply. Significant capital investments, high operational and maintenance costs are the main reasons why Maglev Transport Systems (MTS are hardly currently used for the High-Speed Ground Transportation (HSGT. Therefore, this article justifies use of Theory of Complex Optimization of Transport (TCOT, developed by one of the co-authors, to reduce MTS costs. Methodology. According to TCOT, authors developed an abstract model of the generalized transport system (AMSTG. This model mathematically determines the optimal balance between all components of the system and thus provides the ultimate adaptation of any transport systems to the conditions of its application. To identify areas for effective use of MTS, by TCOT, the authors developed a dynamic model of distribution and expansion of spheres of effective use of transport systems (DMRRSEPTS. Based on this model, the most efficient transport system was selected for each individual track. The main estimated criterion at determination of efficiency of application of MTS is the size of the specific transportation tariff received from calculation of payback of total given expenses to a standard payback period or term of granting the credit. Findings. The completed multiple calculations of four types of MTS: TRANSRAPID, MLX01, TRANSMAG and TRANSPROGRESS demonstrated efficiency of the integrated optimization of the parameters of such systems. This research made possible expending the scope of effective usage of MTS in about 2 times. The achieved results were presented at many international conferences in Germany, Switzerland, United States, China, Ukraine, etc. Using MTS as an

  13. Genome-Wide Identification and Expression Profiling of ATP-Binding Cassette (ABC) Transporter Gene Family in Pineapple (Ananas comosus (L.) Merr.) Reveal the Role of AcABCG38 in Pollen Development.

    Science.gov (United States)

    Chen, Piaojuan; Li, Yi; Zhao, Lihua; Hou, Zhimin; Yan, Maokai; Hu, Bingyan; Liu, Yanhui; Azam, Syed Muhammad; Zhang, Ziyan; Rahman, Zia Ur; Liu, Liping; Qin, Yuan

    2017-01-01

    Pineapple ( Ananas comosus L .) cultivation commonly relies on asexual reproduction which is easily impeded by many factors in agriculture production. Sexual reproduction might be a novel approach to improve the pineapple planting. However, genes controlling pineapple sexual reproduction are still remain elusive. In different organisms a conserved superfamily proteins known as ATP binding cassette (ABC) participate in various biological processes. Whereas, till today the ABC gene family has not been identified in pineapple. Here 100 ABC genes were identified in the pineapple genome and grouped into eight subfamilies (5 ABCAs , 20 ABCB s, 16 ABCCs , 2 ABCDs , one ABCEs , 5 ABCFs , 42 ABCGs and 9 ABCIs ). Gene expression profiling revealed the dynamic expression pattern of ABC gene family in various tissues and different developmental stages. AcABCA5, AcABCB6, AcABCC4 , AcABCC7 , AcABCC9 , AcABCG26 , AcABCG38 and AcABCG42 exhibited preferential expression in ovule and stamen. Over-expression of AcABCG38 in the Arabidopsis double mutant abcg1-2abcg16-2 partially restored its pollen abortion defects, indicating that AcABCG38 plays important roles in pollen development. Our study on ABC gene family in pineapple provides useful information for developing sexual pineapple plantation which could be utilized to improve pineapple agricultural production.

  14. Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes.

    OpenAIRE

    Kegler-Ebo, D M; Docktor, C M; DiMaio, D

    1994-01-01

    We describe codon cassette mutagenesis, a simple method of mutagenesis that uses universal mutagenic cassettes to deposit single codons at specific sites in double-stranded DNA. A target molecule is first constructed that contains a blunt, double-strand break at the site targeted for mutagenesis. A double-stranded mutagenic codon cassette is then inserted at the target site. Each mutagenic codon cassette contains a three base pair direct terminal repeat and two head-to-head recognition sequen...

  15. Integrated transport and renewable energy systems

    DEFF Research Database (Denmark)

    Mathiesen, B.V.; Lund, H.; Nørgård, Per Bromand

    2007-01-01

    Governments worldwide aim at reducing CO2 emissions and expanding renewable energy. A key element in achieving such a goal is to use renewable energy in transport such as biofuels. However, efforts to promote single transport technologies and single fuels only represent a partial solution...... transport. It is concluded that a 100 per cent renewable energy transport system is possible but is connected to significant challenges in the path towards it. Biomass is a limited resource and it is important to avoid effecting the production. The integration of the transport with the remaining energy....... No single technology can solve the problem of ever increasing CO2 emissions from transport. Transport must be integrated into energy planning, as electricity and heating. In this paper, a coherent effort to integrate transport into energy planning is proposed, using multiple means promoting sustainable...

  16. Performances of the HL (Hyperloop) transport system

    OpenAIRE

    van Goeverden, C.D.; Milakis, D.; Janic, M.; Konings, J.W.; Cools, M.; Limbourg, S.

    2017-01-01

    This paper deals with an analysis of performances of the HL (Hyperloop) transport system considered as an advanced transport alternative to the existing APT (Air Passenger Transport) and HSR (High Speed Rail) systems. The considered performances are operational, financial, social and environmental. The operational performance include capacity and quality of service provided to the system’s users-passengers with attributes such as door-to-door travel time consisting of the access and egress ti...

  17. Analysis and comparison of transportation security systems

    International Nuclear Information System (INIS)

    Rinne, R.L.

    1976-05-01

    The role of modeling in the analysis of transportation security systems is described. Various modeling approaches are outlined. The conflict model developed in Sandia Laboratories' Transportation Mode Analysis for the NRC Special Safeguards Study is used to demonstrate the capability of models to determine system sensitivities and compare alternatives

  18. Evolutionary relationships of ATP-Binding Cassette (ABC) uptake porters.

    Science.gov (United States)

    Zheng, Wei Hao; Västermark, Åke; Shlykov, Maksim A; Reddy, Vamsee; Sun, Eric I; Saier, Milton H

    2013-05-06

    The ATP-Binding Cassette (ABC) functional superfamily includes integral transmembrane exporters that have evolved three times independently, forming three families termed ABC1, ABC2 and ABC3, upon which monophyletic ATPases have been superimposed for energy-coupling purposes [e.g., J Membr Biol 231(1):1-10, 2009]. The goal of the work reported in this communication was to understand how the integral membrane constituents of ABC uptake transporters with different numbers of predicted or established transmembrane segments (TMSs) evolved. In a few cases, high resolution 3-dimensional structures were available, and in these cases, their structures plus primary sequence analyses allowed us to predict evolutionary pathways of origin. All of the 35 currently recognized families of ABC uptake proteins except for one (family 21) were shown to be homologous using quantitative statistical methods. These methods involved using established programs that compare native protein sequences with each other, after having compared each sequence with thousands of its own shuffled sequences, to gain evidence for homology. Topological analyses suggested that these porters contain numbers of TMSs ranging from four or five to twenty. Intragenic duplication events occurred multiple times during the evolution of these porters. They originated from a simple primordial protein containing 3 TMSs which duplicated to 6 TMSs, and then produced porters of the various topologies via insertions, deletions and further duplications. Except for family 21 which proved to be related to ABC1 exporters, they are all related to members of the previously identified ABC2 exporter family. Duplications that occurred in addition to the primordial 3 → 6 duplication included 5 → 10, 6 → 12 and 10 → 20 TMSs. In one case, protein topologies were uncertain as different programs gave discrepant predictions. It could not be concluded with certainty whether a 4 TMS ancestral protein or a 5 TMS ancestral protein

  19. Fuel cell cassette with compliant seal

    Science.gov (United States)

    Karl, Haltiner, Jr. J.; Anthony, Derose J.; Klotzbach, Darasack C.; Schneider, Jonathan R.

    2017-11-07

    A fuel cell cassette for forming a fuel cell stack along a fuel cell axis includes a cell retainer, a plate positioned axially to the cell retainer and defining a space axially with the cell retainer, and a fuel cell having an anode layer and a cathode layer separated by an electrolyte layer. The outer perimeter of the fuel cell is positioned in the space between the plate and the cell retainer, thereby retaining the fuel cell and defining a cavity between the cell retainer, the fuel cell, and the plate. The fuel cell cassette also includes a seal disposed within the cavity for sealing the edge of the fuel cell. The seal is compliant at operational temperatures of the fuel cell, thereby allowing lateral expansion and contraction of the fuel cell within the cavity while maintaining sealing at the edge of the fuel cell.

  20. The stability of cassette walls in compression

    Science.gov (United States)

    Voutay, Pierre-Arnaud

    Much research into the behaviour of cold formed steel columns in the last decade has focused on channel sections undergoing local, distortional and overall buckling. Light gauge steel cassette sections are a particular form of channel section which offers an alternative form of load-bearing wall assembly for use in low-rise steel framed construction. Cassette wall sections possess wide and slender flanges so that, by including intermediate stiffeners in these wide flanges, a significant increase in the ultimate load capacity may be achieved. However, the introduction of intermediate stiffeners also increases the number of buckling modes (stiffener buckling) and, therefore complicates the behaviour and increases the risk of interactive buckling between these modes. The work undertaken in this thesis aims to clarify the behaviour of wide flanges in compression with and without intermediate stiffeners. In this research, the distortional mode of web and narrow flange buckling was inhibited by connecting the narrow flanges of the cassettes together at suitable intervals. "Generalised Beam Theory" (GBT), which allows the individual buckling modes to be considered individually and in predetermined combinations, provides a particularly good tool with which to analyse and understand the buckling behaviour of cassette sections with and without intermediate stiffeners. "Generalised Beam Theory" (GBT) is used throughout this work to determine the elastic buckling stress of the sections studied (simply supported stiffened plates, as well as cassette sections). Since the economic design of cold-formed steel sections requires the consideration of post- buckling behaviour, elastic buckling values are not directly comparable with design code values which are usually based on the concept of effective width. Therefore, finite element analysis with both material and geometric nonlinearity has also been carried out in order to obtain the ultimate strength in the critical mode or mode

  1. The transportation operations system: A description

    International Nuclear Information System (INIS)

    Best, R.E.; Danese, F.L.; Dixon, L.D.; Peterson, R.W.; Pope, R.B.

    1990-01-01

    This paper presents a description of the system for transporting radioactive waste that may be deployed to accomplish the assigned system mission, which includes accepting spent nuclear fuel (SNF) and high-level radioactive waste (HLW) from waste generator sites and transporting them to the FWMS destination facilities. The system description presented here contains, in part, irradiated fuel and waste casks, ancillary equipments, truck, rail, and barge transporters, cask and vehicle traffic management organizations, maintenance facilities, and other operations elements. The description is for a fully implemented system, which is not expected to be achieved, however, until several years after initial operations. 6 figs

  2. Sensor Technologies for Intelligent Transportation Systems.

    Science.gov (United States)

    Guerrero-Ibáñez, Juan; Zeadally, Sherali; Contreras-Castillo, Juan

    2018-04-16

    Modern society faces serious problems with transportation systems, including but not limited to traffic congestion, safety, and pollution. Information communication technologies have gained increasing attention and importance in modern transportation systems. Automotive manufacturers are developing in-vehicle sensors and their applications in different areas including safety, traffic management, and infotainment. Government institutions are implementing roadside infrastructures such as cameras and sensors to collect data about environmental and traffic conditions. By seamlessly integrating vehicles and sensing devices, their sensing and communication capabilities can be leveraged to achieve smart and intelligent transportation systems. We discuss how sensor technology can be integrated with the transportation infrastructure to achieve a sustainable Intelligent Transportation System (ITS) and how safety, traffic control and infotainment applications can benefit from multiple sensors deployed in different elements of an ITS. Finally, we discuss some of the challenges that need to be addressed to enable a fully operational and cooperative ITS environment.

  3. Sensor Technologies for Intelligent Transportation Systems

    Science.gov (United States)

    Guerrero-Ibáñez, Juan; Zeadally, Sherali

    2018-01-01

    Modern society faces serious problems with transportation systems, including but not limited to traffic congestion, safety, and pollution. Information communication technologies have gained increasing attention and importance in modern transportation systems. Automotive manufacturers are developing in-vehicle sensors and their applications in different areas including safety, traffic management, and infotainment. Government institutions are implementing roadside infrastructures such as cameras and sensors to collect data about environmental and traffic conditions. By seamlessly integrating vehicles and sensing devices, their sensing and communication capabilities can be leveraged to achieve smart and intelligent transportation systems. We discuss how sensor technology can be integrated with the transportation infrastructure to achieve a sustainable Intelligent Transportation System (ITS) and how safety, traffic control and infotainment applications can benefit from multiple sensors deployed in different elements of an ITS. Finally, we discuss some of the challenges that need to be addressed to enable a fully operational and cooperative ITS environment. PMID:29659524

  4. Sensor Technologies for Intelligent Transportation Systems

    Directory of Open Access Journals (Sweden)

    Juan Guerrero-Ibáñez

    2018-04-01

    Full Text Available Modern society faces serious problems with transportation systems, including but not limited to traffic congestion, safety, and pollution. Information communication technologies have gained increasing attention and importance in modern transportation systems. Automotive manufacturers are developing in-vehicle sensors and their applications in different areas including safety, traffic management, and infotainment. Government institutions are implementing roadside infrastructures such as cameras and sensors to collect data about environmental and traffic conditions. By seamlessly integrating vehicles and sensing devices, their sensing and communication capabilities can be leveraged to achieve smart and intelligent transportation systems. We discuss how sensor technology can be integrated with the transportation infrastructure to achieve a sustainable Intelligent Transportation System (ITS and how safety, traffic control and infotainment applications can benefit from multiple sensors deployed in different elements of an ITS. Finally, we discuss some of the challenges that need to be addressed to enable a fully operational and cooperative ITS environment.

  5. Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes.

    Science.gov (United States)

    Kegler-Ebo, D M; Docktor, C M; DiMaio, D

    1994-05-11

    We describe codon cassette mutagenesis, a simple method of mutagenesis that uses universal mutagenic cassettes to deposit single codons at specific sites in double-stranded DNA. A target molecule is first constructed that contains a blunt, double-strand break at the site targeted for mutagenesis. A double-stranded mutagenic codon cassette is then inserted at the target site. Each mutagenic codon cassette contains a three base pair direct terminal repeat and two head-to-head recognition sequences for the restriction endonuclease Sapl, an enzyme that cleaves outside of its recognition sequence. The intermediate molecule containing the mutagenic cassette is then digested with Sapl, thereby removing most of the mutagenic cassette, leaving only a three base cohesive overhang that is ligated to generate the final insertion or substitution mutation. A general method for constructing blunt-end target molecules suitable for this approach is also described. Because the mutagenic cassette is excised during this procedure and alters the target only by introducing the desired mutation, the same cassette can be used to introduce a particular codon at all target sites. Each cassette can deposit two different codons, depending on the orientation in which it is inserted into the target molecule. Therefore, a series of eleven cassettes is sufficient to insert all possible amino acids at any constructed target site. Thus codon cassettes are 'off-the-shelf' reagents, and this methodology should be a particularly useful and inexpensive approach for subjecting multiple different positions in a protein sequence to saturation mutagenesis.

  6. Functional Diversity of Tandem Substrate-Binding Domains in ABC Transporters from Pathogenic Bacteria

    NARCIS (Netherlands)

    Fulyani, Faizah; Schuurman-Wolters, Gea K.; Vujicic - Zagar, Andreja; Guskov, Albert; Slotboom, Dirk-Jan; Poolman, Bert

    2013-01-01

    The ATP-binding cassette (ABC) transporter GInPQ is an essential uptake system for amino acids in gram-positive pathogens and related nonpathogenic bacteria. The transporter has tandem substrate-binding domains (SBDs) fused to each transmembrane domain, giving rise to four SBDs per functional

  7. ROLE OF ATP BINDING CASSETTE SUB-FAMILY MEMBER 2 (ABCG2) IN MOUSE EMBRYONIC STEM CELL DEVELOPMENT.

    Science.gov (United States)

    ATP binding cassette sub-family member 2 (ABCG2), is a member of the ABC transporter superfamily and a principal xenobiotic transporter. ABCG2 is also highly expressed in certain stem cell populations where it is thought to be related to stem cell plasticity, although the role o...

  8. Driverless operation for public passenger transport systems

    Energy Technology Data Exchange (ETDEWEB)

    Kehl, R. [Siemens AG, Erlangen (Germany). Bereich Verkehrstechnik

    2001-07-01

    The author presents the automation of new and existing lines as a possible solution to the twin problems of the growing need for public transport and the threat of collapse facing many public transport systems in the big conurbations as they wrestle against overloading. It emerges that automatic, driverless operation is a suitable approach to making systems more flexible and more attractive. Automation can increase the capacities of existing systems significantly and thus help them gain more passengers. (orig.)

  9. Operating control techniques for maglev transport systems

    Energy Technology Data Exchange (ETDEWEB)

    Kraft, K H; Schnieder, E

    1984-06-01

    The technical and operational possibilities of magnetic levitation transport systems can only be fully exploited by introducing 'intelligent' control systems which ensure automatic and trouble-free train running. The solution of exacting requirements in the fields of traction dynamics, security and control as well as information gathering transmission and processing is an important prior condition in that respect. The authors report here on the present state of research and development in operating control techniques applicable to maglev transport systems.

  10. 49 CFR 37.33 - Airport transportation systems.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Airport transportation systems. 37.33 Section 37.33 Transportation Office of the Secretary of Transportation TRANSPORTATION SERVICES FOR INDIVIDUALS WITH DISABILITIES (ADA) Applicability § 37.33 Airport transportation systems. (a) Transportation...

  11. Integrated design for space transportation system

    CERN Document Server

    Suresh, B N

    2015-01-01

    The book addresses the overall integrated design aspects of a space transportation system involving several disciplines like propulsion, vehicle structures, aerodynamics, flight mechanics, navigation, guidance and control systems, stage auxiliary systems, thermal systems etc. and discusses the system approach for design, trade off analysis, system life cycle considerations, important aspects in mission management, the risk assessment, etc. There are several books authored to describe the design aspects of various areas, viz., propulsion, aerodynamics, structures, control, etc., but there is no book which presents space transportation system (STS) design in an integrated manner. This book attempts to fill this gap by addressing systems approach for STS design, highlighting the integrated design aspects, interactions between various subsystems and interdependencies. The main focus is towards the complex integrated design to arrive at an optimum, robust and cost effective space transportation system. The orbit...

  12. RELIABILITY OF TRANSPORTATION SYSTEMS OF ROCK HAPS

    Directory of Open Access Journals (Sweden)

    A. Stepanov

    2009-01-01

    Full Text Available The ways of increasing of exploitation reliability of dump trucks with the aim of increasing of effectiveness of exploitation of transportation systems of rock heaps at coal mines.

  13. Intelligent Transportation Systems statewide architecture : final report.

    Science.gov (United States)

    2003-06-01

    This report describes the development of Kentuckys Statewide Intelligent Transportation Systems (ITS) Architecture. The process began with the development of an ITS Strategic Plan in 1997-2000. A Business Plan, developed in 2000-2001, translated t...

  14. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ.

    Science.gov (United States)

    Aida, Tomomi; Nakade, Shota; Sakuma, Tetsushi; Izu, Yayoi; Oishi, Ayu; Mochida, Keiji; Ishikubo, Harumi; Usami, Takako; Aizawa, Hidenori; Yamamoto, Takashi; Tanaka, Kohichi

    2016-11-28

    Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without long homology arms mediated by microhomology-mediated end-joining. Here, we identified exonuclease 1 (Exo1) as an enhancer for PITCh in human cells. By combining the Exo1 and PITCh-directed donor vectors, we achieved convenient one-step knock-in of gene cassettes and floxed allele both in human cells and mouse zygotes. Our results provide a technical platform for high-throughput knock-in.

  15. Systemic Analysis Approaches for Air Transportation

    Science.gov (United States)

    Conway, Sheila

    2005-01-01

    Air transportation system designers have had only limited success using traditional operations research and parametric modeling approaches in their analyses of innovations. They need a systemic methodology for modeling of safety-critical infrastructure that is comprehensive, objective, and sufficiently concrete, yet simple enough to be used with reasonable investment. The methodology must also be amenable to quantitative analysis so issues of system safety and stability can be rigorously addressed. However, air transportation has proven itself an extensive, complex system whose behavior is difficult to describe, no less predict. There is a wide range of system analysis techniques available, but some are more appropriate for certain applications than others. Specifically in the area of complex system analysis, the literature suggests that both agent-based models and network analysis techniques may be useful. This paper discusses the theoretical basis for each approach in these applications, and explores their historic and potential further use for air transportation analysis.

  16. Disinfection Effect of Film Cassettes by Ultraviolet Irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Kweon, Dae Cheol; Park, Peom [Ajou Univ., Suwon (Korea, Republic of)

    2001-12-15

    A bacteria infection on film cassette contact surface was examined at the diagnostic radiology department. Studies have demonstrated a bactericidal effect of ultraviolet irradiation, and to assess the contamination level on film cassette contact surface as a predictor of patient prevent from nosocomial infection. The study showed that the laboratory result was identified non-pathologic and pathologic bacterial in the five different cassette size of the contact surface. Film cassettes were exposed to ultraviolet light for 1, 2 and 3 minutes. Ultraviolet light disinfection practices suitable for bacteria. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. In conclusion, ultraviolet irradiate on film cassette over the surface more than 2 minutes. Ultraviolet dose of 1565 {mu}W {center_dot} s/cm{sup 2}Win in 30 second relative to ultraviolet dose in time.

  17. Disinfection Effect of Film Cassettes by Ultraviolet Irradiation

    International Nuclear Information System (INIS)

    Kweon, Dae Cheol; Park, Peom

    2001-01-01

    A bacteria infection on film cassette contact surface was examined at the diagnostic radiology department. Studies have demonstrated a bactericidal effect of ultraviolet irradiation, and to assess the contamination level on film cassette contact surface as a predictor of patient prevent from nosocomial infection. The study showed that the laboratory result was identified non-pathologic and pathologic bacterial in the five different cassette size of the contact surface. Film cassettes were exposed to ultraviolet light for 1, 2 and 3 minutes. Ultraviolet light disinfection practices suitable for bacteria. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. In conclusion, ultraviolet irradiate on film cassette over the surface more than 2 minutes. Ultraviolet dose of 1565 μW · s/cm 2 Win in 30 second relative to ultraviolet dose in time

  18. The WIPP transportation system: Dedicated to safety

    International Nuclear Information System (INIS)

    Ward, T.; McFadden, M.

    1993-01-01

    When developing a transportation system to transport transuranic (TRU) waste from ten widely-dispersed generator sites, the Department of Energy (DOE) recognized and addressed many challenges. Shipments of waste to the Waste Isolation Pilot Plant (WIPP) were to cover a twenty-five year period and utilize routes covering over twelve thousand miles in twenty-three states. Enhancing public safety by maximizing the payload, thus reducing the number of shipments, was the primary objective. To preclude the requirement for overweight permits, the DOE started with a total shipment weight limit of 80,000 pounds and developed an integrated transportation system consisting of a Type ''B'' package to transport the material, a lightweight tractor and trailer, stringent driver requirements, and a shipment tracking system referred to as ''TRANSCOM''

  19. The WIPP transportation system: Demonstrated readiness

    International Nuclear Information System (INIS)

    Ward, T.R.; Spooner, R.

    1991-01-01

    The Department of Energy (DOE) has developed an integrated transportation system to transport transuranic (TRU) waste from ten widely-dispersed generator sites to the Waste Isolation Pilot Plant (WIPP). The system consists of a Type B container, a specially- designed trailer, a lightweight tractor, the DOE ''TRANSCOM'' vehicle tracking system, and uniquely qualified and highly-trained drivers. In June of 1989, the National Academy of Sciences reviewed the transportation system and concluded that: ''The system proposed for transportation of TRU waste to WIPP is safer than that employed for any other hazardous material in the United States today and will reduce risk to very low levels'' (emphasis added). The next challenge facing the DOE was demonstrating that this system was ready to transport the TRU waste to the WIPP site efficiently and in the safest manner possible. Not only did the DOE feel that is was necessary to convince itself that the system was safe, but also representatives of the 20 states through which it would travel

  20. The WIPP transportation system: Demonstrated readiness

    International Nuclear Information System (INIS)

    Ward, T.R.; Spooner, R.

    1991-01-01

    The Department of Energy (DOE) has developed an integrated transportation system to transport transuranic (TRU) waste from ten widely-dispersed generator sites to the Waste Isolation Pilot Plant (WIPP). The system consists of a Type B container, a specially-designed trailer, a lightweight tractor, the DOE ''TRANSCOM'' vehicle tracing system, and uniquely qualified and highly-trained drivers. In June of 1989, the National Academy of Sciences reviewed the transportation system and concluded that: ''The system proposed for transportation of TRU waste to WIPP is safer than that employed for any other hazardous material in the United States today and will reduce risk to very low levels.'' The next challenge facing the DOE was demonstrating that this system was ready to transport the TRU waste to the WIPP site in the safest manner possible. Not only did the DOE feel that it was necessary to convince itself that the system was safe, but also representatives of the 23 states through which it traveled

  1. 49 CFR 37.25 - University transportation systems.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false University transportation systems. 37.25 Section 37.25 Transportation Office of the Secretary of Transportation TRANSPORTATION SERVICES FOR INDIVIDUALS WITH DISABILITIES (ADA) Applicability § 37.25 University transportation systems. (a...

  2. A study on contamination and disinfection of film cassette

    International Nuclear Information System (INIS)

    Kweon, Dae Cheol; Chung, Kyung Mo; Choi, Ji Won

    2000-01-01

    In July 2000, a bacteria infection on film cassette contact surface was examined at the diagnostic radiology department of the S. hospital. The objective of this study was to assess the contamination level on film cassette contact surface as a predictor of patient to prevent from nosocomial infection. The study showed that the laboratory result was identified non-pathologic bacterial in the four different cassette size of the contact surface. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. Also the education of nosocomial infection for radiographer will be required

  3. A study on contamination and disinfection of film cassette

    Energy Technology Data Exchange (ETDEWEB)

    Kweon, Dae Cheol; Chung, Kyung Mo [Seoul National University Hospital, Seoul (Korea, Republic of); Choi, Ji Won [University of Sydney, Sydney (Australia)

    2000-04-15

    In July 2000, a bacteria infection on film cassette contact surface was examined at the diagnostic radiology department of the S. hospital. The objective of this study was to assess the contamination level on film cassette contact surface as a predictor of patient to prevent from nosocomial infection. The study showed that the laboratory result was identified non-pathologic bacterial in the four different cassette size of the contact surface. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. Also the education of nosocomial infection for radiographer will be required.

  4. Quantum Transport in Mesoscopic Systems

    Indian Academy of Sciences (India)

    voltage bias, the tunneling of the electron from the lead to the dot and vice versa will happen very rarely. Then two successive ..... A typical mesoscopic quantum dot system (a small drop- .... dynamical behavior of the distribution function of the.

  5. Activation of liver X receptor decreases atherosclerosis in Ldlr⁻/⁻ mice in the absence of ATP-binding cassette transporters A1 and G1 in myeloid cells

    NARCIS (Netherlands)

    Kappus, Mojdeh S.; Murphy, Andrew J.; Abramowicz, Sandra; Ntonga, Vusisizwe; Welch, Carrie L.; Tall, Alan R.; Westerterp, Marit

    2014-01-01

    Liver X receptor (LXR) activators decrease atherosclerosis in mice. LXR activators (1) directly upregulate genes involved in reverse cholesterol transport and (2) exert anti-inflammatory effects mediated by transrepression of nuclear factor-κB target genes. We investigated whether myeloid cell

  6. Inactive trials of transport systems: phase II

    International Nuclear Information System (INIS)

    Haberlin, M.M.; Hardy, A.R.; Kennedy, S.T.

    1986-11-01

    Progress made during 1984-85 is reviewed in four sections: the design and installation of a stainless steel working floor in the mock-up of a crate handling and size reduction facility; the detailed evaluation of a single air pad of the type used on commercial air-transporter; an experimental programme designed to examine the problems associated with the operation of a commercial air-transporter; the design, manufacture and commissioning trials of two powered conveyor units which when combined complete a remotely operated transfer system for transporting crated waste into and within the mock-up facility. (author)

  7. Not planning a sustainable transport system

    International Nuclear Information System (INIS)

    Finnveden, Göran; Åkerman, Jonas

    2014-01-01

    The overall objective of the Swedish transport policy is to ensure the economically efficient and sustainable provision of transport services for people and business throughout the country. More specifically, the transport sector shall, among other things, contribute to the achievement of environmental quality objectives in which the development of the transport system plays an important role in the achievement of the objectives. The aim of this study is to analyse if current transport planning supports this policy. This is done by analysing two recent cases: the National Infrastructure Plan 2010–2021, and the planning of Bypass Stockholm, a major road investment. Our results show that the plans are in conflict with several of the environmental quality objectives. Another interesting aspect of the planning processes is that the long-term climate goals are not included in the planning processes, neither as a clear goal nor as factor that will influence future transport systems. In this way, the long-term sustainability aspects are not present in the planning. We conclude that the two cases do not contribute to a sustainable transport system. Thus, several changes must be made in the processes, including putting up clear targets for emissions. Also, the methodology for the environmental assessments needs to be further developed and discussed. - Highlights: • Two cases are studied to analyse if current planning supports a sustainable transport system. • Results show that the plans are in conflict with several of the environmental quality objectives. • Long-term climate goals are not included in the planning processes. • Current practices do not contribute to a sustainable planning processes. • Methodology and process for environmental assessments must be further developed and discussed

  8. Not planning a sustainable transport system

    Energy Technology Data Exchange (ETDEWEB)

    Finnveden, Göran, E-mail: goran.finnveden@abe.kth.se; Åkerman, Jonas

    2014-04-01

    The overall objective of the Swedish transport policy is to ensure the economically efficient and sustainable provision of transport services for people and business throughout the country. More specifically, the transport sector shall, among other things, contribute to the achievement of environmental quality objectives in which the development of the transport system plays an important role in the achievement of the objectives. The aim of this study is to analyse if current transport planning supports this policy. This is done by analysing two recent cases: the National Infrastructure Plan 2010–2021, and the planning of Bypass Stockholm, a major road investment. Our results show that the plans are in conflict with several of the environmental quality objectives. Another interesting aspect of the planning processes is that the long-term climate goals are not included in the planning processes, neither as a clear goal nor as factor that will influence future transport systems. In this way, the long-term sustainability aspects are not present in the planning. We conclude that the two cases do not contribute to a sustainable transport system. Thus, several changes must be made in the processes, including putting up clear targets for emissions. Also, the methodology for the environmental assessments needs to be further developed and discussed. - Highlights: • Two cases are studied to analyse if current planning supports a sustainable transport system. • Results show that the plans are in conflict with several of the environmental quality objectives. • Long-term climate goals are not included in the planning processes. • Current practices do not contribute to a sustainable planning processes. • Methodology and process for environmental assessments must be further developed and discussed.

  9. Transportable Vitrification System Demonstration on Mixed Waste

    International Nuclear Information System (INIS)

    Zamecnik, J.R.; Whitehouse, J.C.; Wilson, C.N.; Van Ryn, F.R.

    1998-01-01

    This paper describes preliminary results from the first demonstration of the Transportable Vitrification System (TVS) on actual mixed waste. The TVS is a fully integrated, transportable system for the treatment of mixed and low-level radioactive wastes. The demonstration was conducted at Oak Ridge's East Tennessee Technology Park (ETTP), formerly known as the K-25 site. The purpose of the demonstration was to show that mixed wastes could be vitrified safely on a 'field' scale using joule-heated melter technology and obtain information on system performance, waste form durability, air emissions, and costs

  10. Pathogen transport in groundwater systems: contrasts with traditional solute transport

    Science.gov (United States)

    Hunt, Randall J.; Johnson, William P.

    2017-06-01

    Water quality affects many aspects of water availability, from precluding use to societal perceptions of fit-for-purpose. Pathogen source and transport processes are drivers of water quality because they have been responsible for numerous outbreaks resulting in large economic losses due to illness and, in some cases, loss of life. Outbreaks result from very small exposure (e.g., less than 20 viruses) from very strong sources (e.g., trillions of viruses shed by a single infected individual). Thus, unlike solute contaminants, an acute exposure to a very small amount of contaminated water can cause immediate adverse health effects. Similarly, pathogens are larger than solutes. Thus, interactions with surfaces and settling become important even as processes important for solutes such as diffusion become less important. These differences are articulated in "Colloid Filtration Theory", a separate branch of pore-scale transport. Consequently, understanding pathogen processes requires changes in how groundwater systems are typically characterized, where the focus is on the leading edges of plumes and preferential flow paths, even if such features move only a very small fraction of the aquifer flow. Moreover, the relatively short survival times of pathogens in the subsurface require greater attention to very fast (solute transport mechanisms discussed here, a more encompassing view of water quality and source water protection is attained. With this more holistic view and theoretical understanding, better evaluations can be made regarding drinking water vulnerability and the relation between groundwater and human health.

  11. High-Energy Beam Transport system

    International Nuclear Information System (INIS)

    Melson, K.E.; Farrell, J.A.; Liska, D.J.

    1979-01-01

    The High-Energy Beam Transport (HEBT) system for the Fusion Materials Irradiation Test (FMIT) Facility is to be installed at the Hanford Engineering Development Laboratory (HEDL) at Richland, Washington. The linear accelerator must transport a large emittance, high-current, high-power, continuous-duty deuteron beam with a large energy spread either to a lithium target or a beam stop. A periodic quadrupole and bending-magnet system provides the beam transport and focusing on target with small beam aberrations. A special rf cavity distributes the energy in the beam so that the Bragg Peak is distributed within the lithium target. Operation of the rf control system, the Energy Dispersion Cavity (EDC), and the beam transport magnets is tested on the beam stop during accelerator turn-on. Characterizing the beam will require extensions of beam diagnostic techniques and noninterceptive sensors. Provisions are being made in the facility for suspending the transport system from overhead supports using a cluster system to simplify maintenance and alignment techniques

  12. Advanced public transportation systems : evaluation guidelines

    Science.gov (United States)

    1994-01-01

    The Federal Transit Administration has developed the Advanced Public Transportation Systems (APTS) Program which is an integral part of the overall U.S. DOT Intelligent Vehicle Highway Systems (IVHS) effort. A major aim of the APTS Program is to prom...

  13. Carbon transport in sodium systems

    International Nuclear Information System (INIS)

    Martin Espigares, M.; Lapena, J.; La Torre, M. de

    1983-01-01

    Carbon activities in dynamic non isothermal sodium system are determined using an equilibratium method. Foils of Fe-18 w% Cr-8 W% Ni alloy with low carbon content (in the as received condition) are exposed to dynamic liquid sodium in the temperature range between 450 0 C and 700 0 C. The analysis was used to evaluate the carburization-decarburization behaviour of type 304 stainless steel exposed to sodium. (author)

  14. The Integrated Air Transportation System Evaluation Tool

    Science.gov (United States)

    Wingrove, Earl R., III; Hees, Jing; Villani, James A.; Yackovetsky, Robert E. (Technical Monitor)

    2002-01-01

    Throughout U.S. history, our nation has generally enjoyed exceptional economic growth, driven in part by transportation advancements. Looking forward 25 years, when the national highway and skyway systems are saturated, the nation faces new challenges in creating transportation-driven economic growth and wealth. To meet the national requirement for an improved air traffic management system, NASA developed the goal of tripling throughput over the next 20 years, in all weather conditions while maintaining safety. Analysis of the throughput goal has primarily focused on major airline operations, primarily through the hub and spoke system.However, many suggested concepts to increase throughput may operate outside the hub and spoke system. Examples of such concepts include the Small Aircraft Transportation System, civil tiltrotor, and improved rotorcraft. Proper assessment of the potential contribution of these technologies to the domestic air transportation system requires a modeling capability that includes the country's numerous smaller airports, acting as a fundamental component of the National Air space System, and the demand for such concepts and technologies. Under this task for NASA, the Logistics Management Institute developed higher fidelity demand models that capture the interdependence of short-haul air travel with other transportation modes and explicitly consider the costs of commercial air and other transport modes. To accomplish this work, we generated forecasts of the distribution of general aviation based aircraft and GA itinerant operations at each of nearly 3.000 airport based on changes in economic conditions and demographic trends. We also built modules that estimate the demand for travel by different modes, particularly auto, commercial air, and GA. We examined GA demand from two perspectives: top-down and bottom-up, described in detail.

  15. LCLS-II CRYOMODULE TRANSPORT SYSTEM TESTING

    Energy Technology Data Exchange (ETDEWEB)

    Huque, Naeem [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); Daly, Edward F. [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); McGee, Michael W. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2018-04-01

    The Cryomodules (CM) for the Linear Coherent Light Source II (LCLS-II) will be shipped to SLAC (Menlo Park, California) from JLab (Newport News, Virginia) and FNAL (Batavia, Illinois). A transportation system has been designed and built to safely transport the CMs over the road. It uses an array of helical isolator springs to attenuate shocks on the CM to below 1.5g in all directions. The system rides on trailers equipped with Air-Ride suspension, which attenuates vibration loads. The prototype LCLS-II CM (pCM) was driven 750 miles to test the transport system; shock loggers recorded the shock attenuation on the pCM and vacuum gauges were used to detect any compromises in beamline vacuum. Alignment measurements were taken before and after the trip to check whether cavity positions had shifted beyond the ± 0.2mm spec. Passband frequencies and cavity gradients were measured at 2K at the Cryomodule Test Facility (CMTF) at JLab to identify any degradation of CM performance after transportation. The transport system was found to have safely carried the CM and is cleared to begin shipments from JLab and FNAL to SLAC.

  16. Transport modeling: An artificial immune system approach

    Directory of Open Access Journals (Sweden)

    Teodorović Dušan

    2006-01-01

    Full Text Available This paper describes an artificial immune system approach (AIS to modeling time-dependent (dynamic, real time transportation phenomenon characterized by uncertainty. The basic idea behind this research is to develop the Artificial Immune System, which generates a set of antibodies (decisions, control actions that altogether can successfully cover a wide range of potential situations. The proposed artificial immune system develops antibodies (the best control strategies for different antigens (different traffic "scenarios". This task is performed using some of the optimization or heuristics techniques. Then a set of antibodies is combined to create Artificial Immune System. The developed Artificial Immune transportation systems are able to generalize, adapt, and learn based on new knowledge and new information. Applications of the systems are considered for airline yield management, the stochastic vehicle routing, and real-time traffic control at the isolated intersection. The preliminary research results are very promising.

  17. The transport system for natural gas

    International Nuclear Information System (INIS)

    Bjoerndalen, Joergen; Nese, Gjermund

    2003-01-01

    In 2002, the actors on the Norwegian shelf in cooperation with the authorities established a new regime for sale and transport of gas. This article deals with some issues of interest relating to this new regime. The transport system for natural gas shows clear signs of being a natural monopoly, which makes it difficult to use the system efficiently. Two main problems of the current way of organizing are pointed out: (1) lack of price and market signals in capacity allocation and (2) unclear incentive effects. The article indicates a possible solution based on the form of organization that is used in the power market

  18. A demonstration of expert systems applications in transportation engineering : volume I, transportation engineers and expert systems.

    Science.gov (United States)

    1987-01-01

    Expert systems, a branch of artificial-intelligence studies, is introduced with a view to its relevance in transportation engineering. Knowledge engineering, the process of building expert systems or transferring knowledge from human experts to compu...

  19. Conflict simulation for surface transport systems

    International Nuclear Information System (INIS)

    Keeton, S.C.; De Laquil, P. III.

    1977-07-01

    An important element in the analysis of transportation safeguards systems is the determination of the outcome of an armed attack against the system. Such information is necessary to understand relationships among the various defender tactics, weapons systems, and adversary attributes. A battle model, SABRES, which can simulate safeguards engagements is under development. This paper briefly describes the first phase of SABRES and presents some examples of its capabilities

  20. Wireless Communications in Smart Rail Transportation Systems

    Directory of Open Access Journals (Sweden)

    César Briso-Rodríguez

    2017-01-01

    Full Text Available Railway, subway, airplane, and other transportation systems have drawn an increasing interest on the use of wireless communications for critical and noncritical services to improve performance, reliability, and passengers experience. Smart transportation systems require the use of critical communications for operation and control, and wideband services can be provided using noncritical communications. High speed train (HST is one of the best test cases for the analysis of communication links and specification of the general requirements for train control and supervision, passenger communications, and onboard and infrastructure wireless sensors. In this paper, we analyze in detail critical and noncritical networks mainly using the HST as a test case. First, the different types of links for smart rail transportation are described, specifying the main requirements of the transportation systems, communications, and their applications for different services. Then, we propose a network architecture and requirements of the communication technologies for critical and noncritical data. Finally, an analysis is made for the future technologies, including the fifth-generation (5G communications, millimeter wave (mmWave, terahertz (THz, and satellites for critical and high-capacity communications in transportation.

  1. AtMRP6/AtABCC6, an ATP-Binding Cassette transporter gene expressed during early steps of seedling development and up-regulated by cadmium in Arabidopsis thaliana

    Science.gov (United States)

    Gaillard, Stéphane; Jacquet, Hélène; Vavasseur, Alain; Leonhardt, Nathalie; Forestier, Cyrille

    2008-01-01

    Background ABC proteins constitute one of the largest families of transporters found in all living organisms. In Arabidopsis thaliana, 120 genes encoding ABC transporters have been identified. Here, the characterization of one member of the MRP subclass, AtMRP6, is described. Results This gene, located on chromosome 3, is bordered by AtMRP3 and AtMRP7. Using real-time quantitative PCR (RT-Q-PCR) and the GUS reporter gene, we found that this gene is essentially expressed during early seedling development, in the apical meristem and at initiation point of secondary roots, especially in xylem-opposite pericycle cells where lateral roots initiate. The level of expression of AtMRP6 in response to various stresses was explored and a significant up-regulation after cadmium (Cd) treatment was detected. Among the three T-DNA insertion lines available from the Salk Institute library, two knock-out mutants, Atmrp6.1 and Atmrp6.2 were invalidated for the AtMRP6 gene. In the presence of Cd, development of leaves was more affected in the mutants than wild-type plants, whereas root elongation and ramification was comparable. Conclusion The position of AtMRP6 on chromosome 3, flanked by two other MRP genes, (all of which being induced by Cd) suggests that AtMRP6 is part of a cluster involved in metal tolerance, although additional functions in planta cannot be discarded. PMID:18307782

  2. The economic potential of a cassette-type-reactor-installed nuclear ice-breaking container ship

    International Nuclear Information System (INIS)

    Kondo, Koichi; Takamasa, Tomoji

    1999-01-01

    An improved cassette-type marine reactor MRX (Marine Reactor X) which is currently researched and developed by the Japan Atomic Energy Research Institute is designed to be easily removed and transferred to another ship. If the reactor in a nuclear-powered ship, which is the reason for its higher cost, were replaced by the cassette-type-MRX, the reusability of the MRX would reduce the cost difference between nuclear-powered and diesel ships. As an investigation of one aspect of a cassette-type MRX, we attempted in this study to do an economic review of an MRX-installed nuclear-powered ice-breaking container ship sailing via the Arctic Ocean. The transportation cost between the Far East and Europe to carry one TEU (twenty-foot-equivalent container unit) over the entire life of the ship for an MRX (which is used for a 20-year period)-installed container ship sailing via the Arctic Ocean is about 70% higher than the Suez Canal diesel ship, carrying 8,000 TEU and sailing at 25 knots, and about 10% higher than the Suez Canal diesel ship carrying 4,000 TEU and sailing at 34 knots. The cost for a cassette-type-MRX (which is used for a 40-year period, removed and transferred to a second ship after being used for 20 years in the first ship)-installed nuclear-powered container ship is about 7% lower than that for the one operated for 20 years. Considering any loss or reduction in sales opportunities through the extension of the transportation period, the nuclear-powered container ship via the Arctic Sea is a more suitable means of transportation than a diesel ship sailing at 25 knots via the Suez Canal when the value of the commodities carried exceeds 2,800 dollars per freight ton. (author)

  3. Crew Transportation System Design Reference Missions

    Science.gov (United States)

    Mango, Edward J.

    2015-01-01

    Contains summaries of potential design reference mission goals for systems to transport humans to andfrom low Earth orbit (LEO) for the Commercial Crew Program. The purpose of this document is to describe Design Reference Missions (DRMs) representative of the end-to-end Crew Transportation System (CTS) framework envisioned to successfully execute commercial crew transportation to orbital destinations. The initial CTS architecture will likely be optimized to support NASA crew and NASA-sponsored crew rotation missions to the ISS, but consideration may be given in this design phase to allow for modifications in order to accomplish other commercial missions in the future. With the exception of NASA’s mission to the ISS, the remaining commercial DRMs are notional. Any decision to design or scar the CTS for these additional non-NASA missions is completely up to the Commercial Provider. As NASA’s mission needs evolve over time, this document will be periodically updated to reflect those needs.

  4. Associations between dru Types and SCCmec Cassettes

    DEFF Research Database (Denmark)

    Bartels, Mette D; Boye, Kit; Oliveira, Duarte C

    2013-01-01

    SCCmec types I to VI. The isolates represented a broad genetic background based on Staphylococcal protein A (spa) typing and multilocus sequence typing (MLST) and included 68 isolates (68 patients) from an outbreak with t024-ST8-IVa and 26 isolates from the same patient. Sequencing identified 53 dru......Molecular typing is an important tool in the investigation of methicillin resistant Staphylococcus aureus (MRSA) outbreaks and in following the evolution of MRSA. The staphylococcal cassette chromosome mec (SCCmec) contains a hypervariable region with a variable number of 40 bp repeats named direct...... repeat units (dru). The dru region has been suggested as a supplementary typing method for MRSA and an international nomenclature exists. The purpose of this study was to investigate the diversity and variability of the dru region in a diverse collection of MRSA. We studied 302 MRSA isolates harbouring...

  5. Developing intelligent transportation systems using the national ITS architecture: an executive edition for senior transportation managers

    Science.gov (United States)

    1998-02-01

    This document has been produced to provide senior transportation managers of state and local departments of transportation with practical guidance for deploying Intelligent Transportation Systems (ITS) consistent with the National ITS Architecture. T...

  6. ATP binding cassette G1-dependent cholesterol efflux during inflammation.

    Science.gov (United States)

    de Beer, Maria C; Ji, Ailing; Jahangiri, Anisa; Vaughan, Ashley M; de Beer, Frederick C; van der Westhuyzen, Deneys R; Webb, Nancy R

    2011-02-01

    ATP binding cassette transporter G1 (ABCG1) mediates the transport of cellular cholesterol to HDL, and it plays a key role in maintaining macrophage cholesterol homeostasis. During inflammation, HDL undergoes substantial remodeling, acquiring lipid changes and serum amyloid A (SAA) as a major apolipoprotein. In the current study, we investigated whether remodeling of HDL that occurs during acute inflammation impacts ABCG1-dependent efflux. Our data indicate that lipid free SAA acts similarly to apolipoprotein A-I (apoA-I) in mediating sequential efflux from ABCA1 and ABCG1. Compared with normal mouse HDL, acute phase (AP) mouse HDL containing SAA exhibited a modest but significant 17% increase in ABCG1-dependent efflux. Interestingly, AP HDL isolated from mice lacking SAA (SAAKO mice) was even more effective in promoting ABCG1 efflux. Hydrolysis with Group IIA secretory phospholipase A(2) (sPLA(2)-IIA) significantly reduced the ability of AP HDL from SAAKO mice to serve as a substrate for ABCG1-mediated cholesterol transfer, indicating that phospholipid (PL) enrichment, and not the presence of SAA, is responsible for alterations in efflux. AP human HDL, which is not PL-enriched, was somewhat less effective in mediating ABCG1-dependent efflux compared with normal human HDL. Our data indicate that inflammatory remodeling of HDL impacts ABCG1-dependent efflux independent of SAA.

  7. Differential privacy in intelligent transportation systems

    NARCIS (Netherlands)

    Kargl, Frank; Friedman, Arik; Boreli, Roksana

    2013-01-01

    In this paper, we investigate how the concept of differential privacy can be applied to Intelligent Transportation Systems (ITS), focusing on protection of Floating Car Data (FCD) stored and processed in central Traffic Data Centers (TDC). We illustrate an integration of differential privacy with

  8. Advanced technologies for intelligent transportation systems

    CERN Document Server

    Picone, Marco; Amoretti, Michele; Zanichelli, Francesco; Ferrari, Gianluigi

    2015-01-01

    This book focuses on emerging technologies in the field of Intelligent Transportation Systems (ITSs) namely efficient information dissemination between vehicles, infrastructures, pedestrians and public transportation systems. It covers the state-of-the-art of Vehicular Ad-hoc Networks (VANETs), with centralized and decentralized (Peer-to-Peer) communication architectures, considering several application scenarios. With a detailed treatment of emerging communication paradigms, including cross networking  and distributed algorithms. Unlike most of the existing books, this book presents a multi-layer overview of information dissemination systems, from lower layers (MAC) to high layers (applications). All those aspects are investigated considering the use of mobile devices, such as smartphones/tablets and embedded systems, i.e. technologies that during last years completely changed the current market, the user expectations, and communication networks. The presented networking paradigms are supported and validate...

  9. Simulating charge transport in flexible systems

    Directory of Open Access Journals (Sweden)

    Timothy Clark

    2015-12-01

    Full Text Available Systems in which movements occur on two significantly different time domains, such as organic electronic components with flexible molecules, require different simulation techniques for the two time scales. In the case of molecular electronics, charge transport is complicated by the several different mechanisms (and theoretical models that apply in different cases. We cannot yet combine time scales of molecular and electronic movement in simulations of real systems. This review describes our progress towards this goal.

  10. A fluorescent cassette-based strategy for engineering multiple domain fusion proteins

    Directory of Open Access Journals (Sweden)

    Khorchid Ahmad

    2003-07-01

    Full Text Available Abstract Background The engineering of fusion proteins has become increasingly important and most recently has formed the basis of many biosensors, protein purification systems, and classes of new drugs. Currently, most fusion proteins consist of three or fewer domains, however, more sophisticated designs could easily involve three or more domains. Using traditional subcloning strategies, this requires micromanagement of restriction enzymes sites that results in complex workaround solutions, if any at all. Results Therefore, to aid in the efficient construction of fusion proteins involving multiple domains, we have created a new expression vector that allows us to rapidly generate a library of cassettes. Cassettes have a standard vector structure based on four specific restriction endonuclease sites and using a subtle property of blunt or compatible cohesive end restriction enzymes, they can be fused in any order and number of times. Furthermore, the insertion of PCR products into our expression vector or the recombination of cassettes can be dramatically simplified by screening for the presence or absence of fluorescence. Conclusions Finally, the utility of this new strategy was demonstrated by the creation of basic cassettes for protein targeting to subcellular organelles and for protein purification using multiple affinity tags.

  11. IOT Based Smart Public Transport System

    Directory of Open Access Journals (Sweden)

    Parag Gawade

    2017-07-01

    Full Text Available Internet of Things IoT joins the objects of this present reality to the virtual world and empowers at whatever time anyplace network for anything that has a turn ON and turn OFF switch. It constitutes to a world where physical things and humans and other living things and virtual information and situations collaborate with each other. Substantial measure of information is created as expansive number of gadgets is associated with the web. So this expansive measure of information must be controlled and changed over to helpful data keeping in mind the end goal to create productive frameworks. In this paper we concentrate on to a urban IoT framework that is utilized to construct Intelligent Transportation System ITS. IoT based intelligent transportation systems are intended to bolster the Smart City vision which intends to utilize the progressed and capable communication systems for the organization of the city and the residents.

  12. Automatic braking system modification for the Advanced Transport Operating Systems (ATOPS) Transportation Systems Research Vehicle (TSRV)

    Science.gov (United States)

    Coogan, J. J.

    1986-01-01

    Modifications were designed for the B-737-100 Research Aircraft autobrake system hardware of the Advanced Transport Operating Systems (ATOPS) Program at Langley Research Center. These modifications will allow the on-board flight control computer to control the aircraft deceleration after landing to a continuously variable level for the purpose of executing automatic high speed turn-offs from the runway. A bread board version of the proposed modifications was built and tested in simulated stopping conditions. Test results, for various aircraft weights, turnoff speed, winds, and runway conditions show that the turnoff speeds are achieved generally with errors less than 1 ft/sec.

  13. Guide to federal intelligent transportation system (ITS) research.

    Science.gov (United States)

    2013-01-01

    The U.S. Department of Transportations (USDOT) Intelligent Transportation System (ITS) Program aims to bring connectivity to transportation through the use of advanced wireless technologies powerful technologies that enable transformative chan...

  14. Operational Efficiency of Public Transport System in Kwara State ...

    African Journals Online (AJOL)

    Operational Efficiency of Public Transport System in Kwara State, Nigeria. ... The paper examines the operations of Public Transport in Nigeria using the Kwara State Transport Service as a case study. ... EMAIL FULL TEXT EMAIL FULL TEXT

  15. Stochastic transport processes in discrete biological systems

    CERN Document Server

    Frehland, Eckart

    1982-01-01

    These notes are in part based on a course for advanced students in the applications of stochastic processes held in 1978 at the University of Konstanz. These notes contain the results of re­ cent studies on the stochastic description of ion transport through biological membranes. In particular, they serve as an introduction to an unified theory of fluctuations in complex biological transport systems. We emphasize that the subject of this volume is not to introduce the mathematics of stochastic processes but to present a field of theoretical biophysics in which stochastic methods are important. In the last years the study of membrane noise has become an important method in biophysics. Valuable information on the ion transport mechanisms in membranes can be obtained from noise analysis. A number of different processes such as the opening and closing of ion channels have been shown to be sources of the measured current or voltage fluctuations. Bio­ logical 'transport systems can be complex. For example, the tr...

  16. Road Transportable Analytical Laboratory (RTAL) system

    International Nuclear Information System (INIS)

    1993-01-01

    The goal of this contractual effort is the development and demonstration of a Road Transportable Analytical Laboratory (RTAL) system to meet the unique needs of the Department of Energy (DOE) for rapid, accurate analysis of a wide variety of hazardous and radioactive contaminants in soil, groundwater, and surface waters. This laboratory system will be designed to provide the field and laboratory analytical equipment necessary to detect and quantify radionuclides, organics, heavy metals and other inorganics, and explosive materials. The planned laboratory system will consist of a set of individual laboratory modules deployable independently or as an interconnected group to meet each DOE site's specific needs

  17. Utilizing ARC EMCS Seedling Cassettes as Highly Versatile Miniature Growth Chambers for Model Organism Experiments

    Science.gov (United States)

    Freeman, John L.; Steele, Marianne K.; Sun, Gwo-Shing; Heathcote, David; Reinsch, S.; DeSimone, Julia C.; Myers, Zachary A.

    2014-01-01

    The aim of our ground testing was to demonstrate the capability of safely putting specific model organisms into dehydrated stasis, and to later rehydrate and successfully grow them inside flight proven ARC EMCS seedling cassettes. The ARC EMCS seedling cassettes were originally developed to support seedling growth during space flight. The seeds are attached to a solid substrate, launched dry, and then rehydrated in a small volume of media on orbit to initiate the experiment. We hypothesized that the same seedling cassettes should be capable of acting as culture chambers for a wide range of organisms with minimal or no modification. The ability to safely preserve live organisms in a dehydrated state allows for on orbit experiments to be conducted at the best time for crew operations and more importantly provides a tightly controlled physiologically relevant growth experiment with specific environmental parameters. Thus, we performed a series of ground tests that involved growing the organisms, preparing them for dehydration on gridded Polyether Sulfone (PES) membranes, dry storage at ambient temperatures for varying periods of time, followed by rehydration. Inside the culture cassettes, the PES membranes were mounted above blotters containing dehydrated growth media. These were mounted on stainless steel bases and sealed with plastic covers that have permeable membrane covered ports for gas exchange. The results showed we were able to demonstrate acceptable normal growth of C.elegans (nematodes), E.coli (bacteria), S.cerevisiae (yeast), Polytrichum (moss) spores and protonemata, C.thalictroides (fern), D.discoideum (amoeba), and H.dujardini (tardigrades). All organisms showed acceptable growth and rehydration in both petri dishes and culture cassettes initially, and after various time lengths of dehydration. At the end of on orbit ISS European Modular Cultivation System experiments the cassettes could be frozen at ultra-low temperatures, refrigerated, or chemically

  18. ATP-binding cassette B10 regulates early steps of heme synthesis.

    Science.gov (United States)

    Bayeva, Marina; Khechaduri, Arineh; Wu, Rongxue; Burke, Michael A; Wasserstrom, J Andrew; Singh, Neha; Liesa, Marc; Shirihai, Orian S; Langer, Nathaniel B; Paw, Barry H; Ardehali, Hossein

    2013-07-19

    Heme plays a critical role in gas exchange, mitochondrial energy production, and antioxidant defense in cardiovascular system. The mitochondrial transporter ATP-binding cassette (ABC) B10 has been suggested to export heme out of the mitochondria and is required for normal hemoglobinization of erythropoietic cells and protection against ischemia-reperfusion injury in the heart; however, its primary function has not been established. The aim of this study was to identify the function of ABCB10 in heme synthesis in cardiac cells. Knockdown of ABCB10 in cardiac myoblasts significantly reduced heme levels and the activities of heme-containing proteins, whereas supplementation with δ-aminolevulinic acid reversed these defects. Overexpression of mitochondrial δ-aminolevulinic acid synthase 2, the rate-limiting enzyme upstream of δ-aminolevulinic acid export, failed to restore heme levels in cells with ABCB10 downregulation. ABCB10 and heme levels were increased by hypoxia, and reversal of ABCB10 upregulation caused oxidative stress and cell death. Furthermore, ABCB10 knockdown in neonatal rat cardiomyocytes resulted in a significant delay of calcium removal from the cytoplasm, suggesting a relaxation defect. Finally, ABCB10 expression and heme levels were altered in failing human hearts and mice with ischemic cardiomyopathy. ABCB10 plays a critical role in heme synthesis pathway by facilitating δ-aminolevulinic acid production or export from the mitochondria. In contrast to previous reports, we show that ABCB10 is not a heme exporter and instead is required for the early mitochondrial steps of heme biosynthesis.

  19. Arterial intelligent transportation systems : infrastructure elements and traveler information requirements.

    Science.gov (United States)

    2009-08-01

    Applying Intelligent Transportation Systems (ITS) to arterial systems allows TxDOT to significantly enhance : transportation system operation efficiency and improve traffic mobility. However, no guidelines are available to : assist TxDOT staff in sel...

  20. Low energy beam transport system developments

    Energy Technology Data Exchange (ETDEWEB)

    Dudnikov, V., E-mail: vadim@muonsinc.com [Muons, Inc., Batavia, IL 60510 (United States); Han, B.; Stockli, M.; Welton, R. [ORNL, Oak Ridge, TN 37831 (United States); Dudnikova, G. [University of Maryland, College Park, MD 3261 (United States); Institute of Computational Technologies SBRAS, Novosibirsk (Russian Federation)

    2015-04-08

    For high brightness beam production it is important to preserve the brightness in the low energy beam transport system (LEBT) used to transport and match the ion beams to the next stage of acceleration, usually an RFQ. While electrostatic focusing can be problematic for high current beam transport, reliable electrostatic LEBT operation has been demonstrated with H{sup −} beams up to 60 mA. Now, however, it is commonly accepted that an optimal LEBT for high current accelerator applications consists of focusing solenoids with space charge compensation. Two-solenoid LEBTs are successfully used for high current (>100 mA) proton beam transport. Preservation of low emittances (~0.15 π mm-mrad) requires the addition of a heavy gas (Xe, Kr), which causes ~5% of proton loss in a 1 m long LEBT. Similar Xe densities would be required to preserve low emittances of H{sup −} beams, but such gas densities cause unacceptably high H{sup −} beam losses. A short LEBT with only one short solenoid, movable for RFQ matching, can be used for reduced negative ion stripping. A strong electrostatic-focusing LEBT has been successfully adopted for transport of high current H{sup −} beams in the SNS Front End. Some modifications of such electrostatic LEBTs are expected to improve the reliable transport of intense positive and negative ion beams without greatly degrading their low emittances. We concentrate on processes that determine the beam brightness degradation and on their prevention. Proposed improvements to the SNS electrostatic LEBT are discussed.

  1. Fuels processing for transportation fuel cell systems

    Science.gov (United States)

    Kumar, R.; Ahmed, S.

    Fuel cells primarily use hydrogen as the fuel. This hydrogen must be produced from other fuels such as natural gas or methanol. The fuel processor requirements are affected by the fuel to be converted, the type of fuel cell to be supplied, and the fuel cell application. The conventional fuel processing technology has been reexamined to determine how it must be adapted for use in demanding applications such as transportation. The two major fuel conversion processes are steam reforming and partial oxidation reforming. The former is established practice for stationary applications; the latter offers certain advantages for mobile systems and is presently in various stages of development. This paper discusses these fuel processing technologies and the more recent developments for fuel cell systems used in transportation. The need for new materials in fuels processing, particularly in the area of reforming catalysis and hydrogen purification, is discussed.

  2. The Efficiency of the Bimodal System Transportation

    Directory of Open Access Journals (Sweden)

    Nada Štrumberger

    2012-10-01

    Full Text Available The development of fast railway results in an increased applicationof Trailer Train bimodal system transportation. Thetraffic costs are multiply reduced, particularly the variablecosts. On the other hand the environmental pollution from exhaustgases is also reduced. Therefore, by the year 2010 cargotransport should be preponderant~v used which would be characterisedby fast electric trains producing less noise, at lowercosts and with clean environment.

  3. Cotton Transportation and Logistics: A Dynamic System

    OpenAIRE

    Robinson, John R.; Park, John L.; Fuller, Stephen

    2007-01-01

    The paper reviews the evolution of U.S. cotton transportation and logistics patterns over the last three decades. There have been many forces of change over this time period, with the largest change being a shift from primarily domestic market destinations to the international market. We describe the pre-1999 system and flow patterns when domestic consumption of U.S. cotton was dominant. We contrast this with current flow patterns as measured by available secondary export data and a sample of...

  4. Assateague Island National Seashore alternative transportation systems planning study and business plan for alternative transportation

    Science.gov (United States)

    2012-08-31

    The purpose of this study was to (1) study the potential expansion of existing alternative transportation systems (bicycle facilities) and development of new alternative transportation systems in and around the Maryland District of Assateague Island ...

  5. Evaluation of alternative public transportation systems in Izmit urban transportation via axiomatic design method

    Directory of Open Access Journals (Sweden)

    Gülşen AKMAN

    2016-02-01

    Full Text Available In the world and in our country, most of urban transportation is performed by public transportation. Public transportation is a system which provides transportation easiness and opportunity to people, not to vehicles. Therefore, giving priority to public transportation system is necessary in organizing urban transportation. In this study, in order to reduce traffic intensity and to facilitate passenger transportation in Izmit urban transportation, It is tried to determine appropriate public transportation system. For this, firstly, alternatives which could be used for public transportation were determined. These alternatives are metro, metrobus, tram, light rail system and monorail. Afterwards, the variables affecting decision making about public transportation were determined. These variables are cost, transportation line features, vehicle characteristics, sensitivity to environment and customer satisfaction. Lastly, most appropriate public transportation system is proposed by using the axiomatic design method. As a result, light trail system and metrobus are determined as the most appropriate alternatives for Izmit public transportation system.Keywords: Urban transportation, Multi criteria decision making, Axiomatic design

  6. The Palm Desert renewable [hydrogen] transportation system

    Energy Technology Data Exchange (ETDEWEB)

    Chamberlin, C.E.; Lehman, P. [Humboldt State Univ., Arcata, CA (United States). Schatz Energy Research Center

    1998-08-01

    This paper describes the Schatz Energy Research Center (SERC) progress on the Palm Desert Renewable Hydrogen Transportation System Project for the period June 1997 through May 1998. The project began in March 1996. The goal of the Palm Desert Project is to develop a clean and sustainable transportation system for a community. The project demonstrates the practical utility of hydrogen as a transportation fuel and the proton exchange membrane (PEM) fuel cell as a vehicle power system. The project includes designing and building 4 fuel cell powered vehicles, a solar hydrogen generating and refueling station, and a fuel cell vehicle diagnostic center. Over this last year, SERC has built a fuel cell powered neighborhood electric vehicle and delivered it to the City of Palm Desert. The design of the hydrogen refueling station is near completion and it is anticipated that construction will be complete in the fall of 1998. The vehicles are currently being refueled at a temporary refueling station. The diagnostic center is being designed and maintenance procedures as well as computer diagnostic programs for the fuel cell vehicles are being developed. City employees are driving the vehicles daily and monitoring data are being collected. The drivers are pleased with the performance of the vehicles.

  7. Pipesworld : applying planning systems to pipeline transportation

    Energy Technology Data Exchange (ETDEWEB)

    Milidiu, R.L.; Santos Liporace, F. [Pontificia Univ. Catolica, Rio de Janeiro (Brazil). Dept. de Informatica

    2004-07-01

    This paper explored issues facing the complex task of managing pipelines that transport large volumes of petroleum products over long distances. Since oil pipelines are generally a few inches wide and several miles long, reasonable amounts of distinct products can be transported with very small loss due to the mixing at liquid boundaries. Optimizing the transportation through oil pipelines in terms of maintenance and environmental safety is a high priority for pipeline operators. This paper presented the Pipesworld model which takes into account features such as product interface constraints, limited product storage capacities and due dates for product delivery. It has been benchmarked as a start-of-art general purpose artificial planning system. This paper also reported the results derived by general purpose artificial intelligence planning systems when applied to the Pipesworld model. It demonstrated how various modelling techniques can be used to enhance the planners performance. Current work in developing Plumber was also presented. This dedicated solver that addresses operational situations uses both general purpose planning techniques as well as domain specific knowledge. When Plumber was incorporated into Pipesworld, its outperformed Fast-Forward, one of the best available general purpose planning systems, suggesting that improved versions of Plumber have the potential to deal with various problem scenarios in pipeline operations. 11 refs., 2 tabs., 3 figs.

  8. The Place of Railway Transport in Romania’s Transport System

    OpenAIRE

    Dãneci-Pãtrãu Daniel; Coca Carmen Elena

    2012-01-01

    The transport activity in all its forms represents one of the most complex sections of an economy, but also an important factor of influence over the quality of life, that is why current preoccupations of international organisms are oriented towards measures meant to develop performing transport systems and compatible with the environment. In the article the main activities and resources of Romania’s railway transport system are presented , the place of the railway transport in the transport ...

  9. Visualizing Mobility of Public Transportation System.

    Science.gov (United States)

    Zeng, Wei; Fu, Chi-Wing; Arisona, Stefan Müller; Erath, Alexander; Qu, Huamin

    2014-12-01

    Public transportation systems (PTSs) play an important role in modern cities, providing shared/massive transportation services that are essential for the general public. However, due to their increasing complexity, designing effective methods to visualize and explore PTS is highly challenging. Most existing techniques employ network visualization methods and focus on showing the network topology across stops while ignoring various mobility-related factors such as riding time, transfer time, waiting time, and round-the-clock patterns. This work aims to visualize and explore passenger mobility in a PTS with a family of analytical tasks based on inputs from transportation researchers. After exploring different design alternatives, we come up with an integrated solution with three visualization modules: isochrone map view for geographical information, isotime flow map view for effective temporal information comparison and manipulation, and OD-pair journey view for detailed visual analysis of mobility factors along routes between specific origin-destination pairs. The isotime flow map linearizes a flow map into a parallel isoline representation, maximizing the visualization of mobility information along the horizontal time axis while presenting clear and smooth pathways from origin to destinations. Moreover, we devise several interactive visual query methods for users to easily explore the dynamics of PTS mobility over space and time. Lastly, we also construct a PTS mobility model from millions of real passenger trajectories, and evaluate our visualization techniques with assorted case studies with the transportation researchers.

  10. Development of cask and transportation system

    International Nuclear Information System (INIS)

    Ro, Seong Gy; Kang, Hee Dong; Lee, Heung Young; Seo, Ki Suk; Koo, Jung Hoe; Jung, Sung Hwan; Yoon, Jung Hyun; Lee, Ju Chan; Bang, Kyung Sik; Baek, Chang Yeol

    1992-03-01

    The major goal of this project is to establish the safe transport system and obtain the necessary data for cask development by during research work for the design and safety test of shipping cask. The analysis technique using computer code for design has been studied in the field of structure, thermal and shielding analysis in this study. And also the test and measurement technology was developed for the measuring system of drop and fire test. It is expected that research activity ensured in this job will enable us to ultilize the basic data for the cask development. (Author)

  11. FFTF Heat Transport System (HTS) component and system design

    International Nuclear Information System (INIS)

    Young, M.W.; Edwards, P.A.

    1980-01-01

    The FFTF Heat Transport Systems and Components designs have been completed and successfully tested at isothermal conditions up to 427 0 C (800 0 F). General performance has been as predicted in the design analyses. Operational flexibility and reliability have been outstanding throughout the test program. The components and systems have been demonstrated ready to support reactor powered operation testing planned later in 1980

  12. Heavy particle transport in sputtering systems

    Science.gov (United States)

    Trieschmann, Jan

    2015-09-01

    This contribution aims to discuss the theoretical background of heavy particle transport in plasma sputtering systems such as direct current magnetron sputtering (dcMS), high power impulse magnetron sputtering (HiPIMS), or multi frequency capacitively coupled plasmas (MFCCP). Due to inherently low process pressures below one Pa only kinetic simulation models are suitable. In this work a model appropriate for the description of the transport of film forming particles sputtered of a target material has been devised within the frame of the OpenFOAM software (specifically dsmcFoam). The three dimensional model comprises of ejection of sputtered particles into the reactor chamber, their collisional transport through the volume, as well as deposition of the latter onto the surrounding surfaces (i.e. substrates, walls). An angular dependent Thompson energy distribution fitted to results from Monte-Carlo simulations is assumed initially. Binary collisions are treated via the M1 collision model, a modified variable hard sphere (VHS) model. The dynamics of sputtered and background gas species can be resolved self-consistently following the direct simulation Monte-Carlo (DSMC) approach or, whenever possible, simplified based on the test particle method (TPM) with the assumption of a constant, non-stationary background at a given temperature. At the example of an MFCCP research reactor the transport of sputtered aluminum is specifically discussed. For the peculiar configuration and under typical process conditions with argon as process gas the transport of aluminum sputtered of a circular target is shown to be governed by a one dimensional interaction of the imposed and backscattered particle fluxes. The results are analyzed and discussed on the basis of the obtained velocity distribution functions (VDF). This work is supported by the German Research Foundation (DFG) in the frame of the Collaborative Research Centre TRR 87.

  13. National Space Transportation System (NSTS) technology needs

    Science.gov (United States)

    Winterhalter, David L.; Ulrich, Kimberly K.

    1990-01-01

    The National Space Transportation System (NSTS) is one of the Nation's most valuable resources, providing manned transportation to and from space in support of payloads and scientific research. The NSTS program is currently faced with the problem of hardware obsolescence, which could result in unacceptable schedule and cost impacts to the flight program. Obsolescence problems occur because certain components are no longer being manufactured or repair turnaround time is excessive. In order to achieve a long-term, reliable transportation system that can support manned access to space through 2010 and beyond, NASA must develop a strategic plan for a phased implementation of enhancements which will satisfy this long-term goal. The NSTS program has initiated the Assured Shuttle Availability (ASA) project with the following objectives: eliminate hardware obsolescence in critical areas, increase reliability and safety of the vehicle, decrease operational costs and turnaround time, and improve operational capability. The strategy for ASA will be to first meet the mandatory needs - keep the Shuttle flying. Non-mandatory changes that will improve operational capability and enhance performance will then be considered if funding is adequate. Upgrade packages should be developed to install within designated inspection periods, grouped in a systematic approach to reduce cost and schedule impacts, and allow the capability to provide a Block 2 Shuttle (Phase 3).

  14. A new type of gene-disruption cassette with a rescue gene for Pichia pastoris.

    Science.gov (United States)

    Shibui, Tatsuro; Hara, Hiroyoshi

    2017-09-01

    Pichia pastoris has been used for the production of many recombinant proteins, and many useful mutant strains have been created. However, the efficiency of mutant isolation by gene-targeting is usually low and the procedure is difficult for those inexperienced in yeast genetics. In order to overcome these issues, we developed a new gene-disruption system with a rescue gene using an inducible Cre/mutant-loxP system. With only short homology regions, the gene-disruption cassette of the system replaces its target-gene locus containing a mutation with a compensatory rescue gene. As the cassette contains the AOX1 promoter-driven Cre gene, when targeted strains are grown on media containing methanol, the DNA fragment, i.e., the marker, rescue and Cre genes, between the mutant-loxP sequences in the cassette is excised, leaving only the remaining mutant-loxP sequence in the genome, and consequently a target gene-disrupted mutant can be isolated. The system was initially validated on ADE2 gene disruption, where the disruption can easily be detected by color-change of the colonies. Then, the system was applied for knocking-out URA3 and OCH1 genes, reported to be difficult to accomplish by conventional gene-targeting methods. All three gene-disruption cassettes with their rescue genes replaced their target genes, and the Cre/mutant-loxP system worked well to successfully isolate their knock-out mutants. This study identified a new gene-disruption system that could be used to effectively and strategically knock out genes of interest, especially whose deletion is detrimental to growth, without using special strains, e.g., deficient in nonhomologous end-joining, in P. pastoris. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1201-1208, 2017. © 2017 American Institute of Chemical Engineers.

  15. Transport in low-dimensional mesoscopic systems

    Energy Technology Data Exchange (ETDEWEB)

    Syzranov, Sergey

    2011-05-05

    The work is devoted to the physics of graphene-based optoelectronics and arrays of Josephson junctions. The first part deals with transport in a graphene p-n junction irradiated by an electromagnetic field. The photocurrent in such device is calculated analytically and compared to those observed in the recent experiments on graphene photodetectors. It is shown that in a clean effectively one-dimensional junction the photocurrent oscillates as a function of gate voltages due to the interference between electron paths accompanied by the resonant photon absorption. The second part of the thesis is devoted to the construction of a Drude-like theory for the transport of Cooper pairs in weakly disordered Josephson networks and to finding the conductivity and the characteristic temperature of the commencement of strong localization. Also, it is shown that the low-temperature superconductor-insulator transition is necessarily of the first order in all 3D and in most 2D systems.

  16. Tomorrow's Transportation Market : Developing an Innovative, Seamless Transportation System

    Science.gov (United States)

    2013-04-17

    With the cost of congestion in the United States estimated to be in the order of $121 billion, transportation planners are under increasing pressure to improve conditions and meet projected demand increases. Harnessing emerging technologies to develo...

  17. Design of a hydraulic ash transport system

    Energy Technology Data Exchange (ETDEWEB)

    Mirgorodskii, V.G.; Mova, M.E.; Korenev, V.E.; Grechikhin, Yu.A. (Donetskii Politekhnicheskii Institut (USSR))

    1990-04-01

    Discusses general design of a hydraulic ash removal system to be employed at the reconstructed six 225 MW blocks of the Mironov State Regional Power Plant in the USSR. The blocks burn low-grade solid fuel with an ash content of up to 40.5%. Large quantities of ash have to be moved from the plant (total ash production 60 t/h, using 570 t/h of water for cooling and moistening). An optimum hydraulic ash transportation system would include a two-section airlift pumping system, shown in a diagram. Technological advantages of using this airlift system are enumerated, including short pipes, reduction in required water quantity and the possibility of siting hydraulic pumps at zero level.

  18. ATP-Binding Cassette Transporters, Atherosclerosis, and Inflammation

    NARCIS (Netherlands)

    Westerterp, Marit; Bochem, Andrea E.; Yvan-Charvet, Laurent; Murphy, Andrew J.; Wang, Nan; Tall, Alan R.

    2014-01-01

    Although recent genome-wide association studies have called into question the causal relationship between high-density lipoprotein (HDL) cholesterol levels and cardiovascular disease, ongoing research in animals and cells has produced increasing evidence that cholesterol efflux pathways mediated by

  19. Accelerating technological change. Towards a more sustainable transport system

    NARCIS (Netherlands)

    van der Vooren, A.

    2014-01-01

    This thesis provides insights into the mechanisms of technological change by capturing the complexity that characterises the current technological transition of the transport system into existing evolutionary models of technological change. The transition towards a more sustainable transport system

  20. Intelligent transport systems (UTS) and driving behaviour: setting the agenda

    NARCIS (Netherlands)

    Heijden, R.E.C.M. van der; Marchau, V.A.W.J.; Thissen, W.A.H.; Wieinga, P.; Pantic, M.; Ludema, M.

    2004-01-01

    The application of intelligent transportation systems (ITS), in particular advanced driver assistance systems (ADAS), is expected to improve the performance of road transportation significantly. Public policy makers, among others, are therefore increasingly interested in the implementation

  1. Selected legal and regulatory concerns affecting domestic energy transportation systems

    International Nuclear Information System (INIS)

    Schuller, C.R.

    1979-07-01

    This report provides assessments of eight legal and regulatory concerns that may affect energy material transportation in the US during the rest of the century: state authority to regulate nuclear materials transport, divestiture of petroleum pipelines from major integrated oil companies, problems affecting the natural gas transportation system, capabilities of energy transportation systems during emergencies, Federal coal pipeline legislation, ability of Federal agencies to anticipate railroad difficulties, abandonment of uneconomic railroad lines, and impact of the Panama Canal treaty upon US energy transportation

  2. Intermodal transport as an integral part of logistics system

    Directory of Open Access Journals (Sweden)

    Agnieszka Bitkowska

    2016-06-01

    Full Text Available The experience of companies that are successful in the carriage of goods prove that intermodal transport is now a major factor in determining the success of logistics system. A modern approach to the transport is based on intermodal transport. The article is based on the method of external observation. It presents the essence of intermodal transport and its benefits. It specifies transportation as an integral part of logistics system.

  3. Transport systems and policies for sustainable cities

    Directory of Open Access Journals (Sweden)

    Vučić Vukan R.

    2008-01-01

    Full Text Available The 20th century witnessed revolutionary developments in transportation technology with major impacts on the form and character of cities. Progress in increasing mobility has brought many benefits as well as serious problems, particularly in deterioration of livability and sustainability. Increase in auto ownership led to serious problems of chronic traffic congestion. Attempts to rebuild cities to provide full accommodation of private cars have led to serious problems of auto dependency and deterioration of cities. Experiences from recent decades have shown that urban transportation is much more complex than usually realized. Livable and sustainable cities require policies that lead to creation of a transportation system consisting of coordinated public transit and private cars, and encourages pedestrian environment and efficient, sustainable development. Great need for better understanding of the complex problems in implementing incentives and disincentives aimed at achieving intermodal balance is emphasized. Brief descriptions of cities which lead in achieving such livable conditions is followed by a summary of lessons and guidelines for the future.

  4. Study of a transportable neutron radiography system

    International Nuclear Information System (INIS)

    Souza, S.N.A. de.

    1991-05-01

    This work presents a study a transportable neutron radiography system for a 185 GBq 241 Am-Be (α, η) source with a neutron yield roughly 1,25 x 10 7 n/s. Studies about moderation, collimation and shielding are showed. In these studies, a calculation using Transport Theory was carried out by means of transport codes ANISN and DOT (3.5). Objectives were: to obtain a maximum and more homogeneous thermal neutron flux in the collimator outlet to the image plain, and an adequate radiation shielding to attend radiological protection rules. With the presented collimator, it was possible to obtain for the thermal neutron flux, at the collimator outlet and next to the image plain, a L/D ratio of 14, for neutron fluxes up to 4,09 x 10 2 n.cm -2 .s -1 . Considering the low intensity of the source, it is a good value. Studies have also been carried out for L/D ratios of 22 and 30, giving thermal neutron fluxes at the image plain of 1,27 x 10 2 n.cm -2 .s -1 and 2,65 x 10 2 n.cm -2 .s -1 , respectively. (author). 30 refs, 39 figs, 9 tabs

  5. X-ray transport and radiation response assessment (XTRRA) experiments at the National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Fournier, K. B., E-mail: fournier2@llnl.gov; Brown, C. G.; Yeoman, M. F.; Compton, S.; Holdener, F. R.; Kemp, G. E.; Blue, B. E. [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551 (United States); Fisher, J. H.; Newlander, C. D.; Gilliam, R. P.; Froula, N. [Fifth Gait Technologies, Inc., 14040 Camden Circle, Huntsville, Alabama 35803 (United States); Seiler, S. W.; Davis, J. F.; Lerch, MAJ. A. [Defense Threat Reduction Agency, 8725 John J. Kingman Road, Fort Belvoir, Virginia 22060-6201 (United States); Hinshelwood, D. [Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375 (United States); Lilly, M. [Dynasen, Inc., 20 Arnold Pl., Goleta, California 93117 (United States)

    2016-11-15

    Our team has developed an experimental platform to evaluate the x-ray-generated stress and impulse in materials. Experimental activities include x-ray source development, design of the sample mounting hardware and sensors interfaced to the National Ignition Facility’s diagnostics insertion system, and system integration into the facility. This paper focuses on the X-ray Transport and Radiation Response Assessment (XTRRA) test cassettes built for these experiments. The test cassette is designed to position six samples at three predetermined distances from the source, each known to within ±1% accuracy. Built-in calorimeters give in situ measurements of the x-ray environment along the sample lines of sight. The measured accuracy of sample responses as well as planned modifications to the XTRRA cassette is discussed.

  6. Operating systems in the air transportation environment.

    Science.gov (United States)

    Cherry, G. W.

    1971-01-01

    Consideration of the problems facing air transport at present, and to be expected in the future. In the Northeast Corridor these problems involve community acceptance, airway and airport congestion and delays, passenger acceptance, noise reduction, and improvements in low-density short-haul economics. In the development of a superior short-haul operating system, terminal-configured vs cruise-configured vehicles are evaluated. CTOL, STOL, and VTOL aircraft of various types are discussed. In the field of noise abatement, it is shown that flight procedural techniques are capable of supplementing ?quiet engine' technology.

  7. Investments and Operation in an Integrated Power and Transport System

    DEFF Research Database (Denmark)

    Juul, Nina; Boomsma, Trine Krogh

    2013-01-01

    This chapter analyses an integrated power and road transport system. For analysing the influences of including passenger road transport in the energy system, a road transport model is developed. Based on this model, the benefits of integration of the two systems and using electric-drive vehicles ...

  8. Third-order TRANSPORT: A computer program for designing charged particle beam transport systems

    International Nuclear Information System (INIS)

    Carey, D.C.; Brown, K.L.; Rothacker, F.

    1995-05-01

    TRANSPORT has been in existence in various evolutionary versions since 1963. The present version of TRANSPORT is a first-, second-, and third-order matrix multiplication computer program intended for the design of static-magnetic beam transport systems. This report discusses the following topics on TRANSPORT: Mathematical formulation of TRANSPORT; input format for TRANSPORT; summaries of TRANSPORT elements; preliminary specifications; description of the beam; physical elements; other transformations; assembling beam lines; operations; variation of parameters for fitting; and available constraints -- the FIT command

  9. Application of the transport system concept to the transport of LSA waste

    International Nuclear Information System (INIS)

    Lombard, J.; Appleton, P.; Libon, H.; Sannen, H.

    1994-01-01

    The aim of this presentation is to illustrate using two examples how a particular special arrangement can be envisaged for the transport of a well defined category of waste according to the ''Transport System Concept''. (authors)

  10. A radioactive waste transportation package monitoring system for normal transport and accident emergency response conditions

    International Nuclear Information System (INIS)

    Brown, G.S.; Cashwell, J.W.; Apple, M.L.

    1993-01-01

    This paper addresses spent fuel and high level waste transportation history and prospects, discusses accident histories of radioactive material transport, discusses emergency responder needs and provides a general description of the Transportation Intelligent Monitoring System (TRANSIMS) design. The key objectives of the monitoring system are twofold: (1) to facilitate effective emergency response to accidents involving a radioactive waste transportation package, while minimizing risk to the public and emergency first-response personnel, and (2) to allow remote monitoring of transportation vehicle and payload conditions to enable research into radioactive material transportation for normal and accident conditions. (J.P.N.)

  11. Road Transportable Analytical Laboratory (RTAL) system

    International Nuclear Information System (INIS)

    Finger, S.M.

    1995-01-01

    U.S. Department of Energy (DOE) facilities around the country have, over the years, become contaminated with radionuclides and a range of organic and inorganic wastes. Many of the DOE sites encompass large land areas and were originally sited in relatively unpopulated regions of the country to minimize risk to surrounding populations. In addition, wastes were sometimes stored underground at the sites in 55-gallon drums, wood boxes or other containers until final disposal methods could be determined. Over the years, these containers have deteriorated, releasing contaminants into the surrounding environment. This contamination has spread, in some cases polluting extensive areas. Remediation of these sites requires extensive sampling to determine the extent of the contamination, to monitor clean-up and remediation progress, and for post-closure monitoring of facilities. The DOE would benefit greatly if it had reliable, road transportable, fully independent laboratory systems that could perform on-site the full range of analyses required. Such systems would accelerate and thereby reduce the cost of clean-up and remediation efforts by (1) providing critical analytical data more rapidly, and (2) eliminating the handling, shipping and manpower associated with sample shipments. The goal of the Road Transportable Analytical Laboratory (RTAL) Project is the development and demonstration of a system to meet the unique needs of the DOE for rapid, accurate analysis of a wide variety of hazardous and radioactive contaminants in soil, groundwater, and surface waters. This laboratory system has been designed to provide the field and laboratory analytical equipment necessary to detect and quantify radionuclides, organics, heavy metals and other inorganic compounds. The laboratory system consists of a set of individual laboratory modules deployable independently or as an interconnected group to meet each DOE site's specific needs

  12. Risk management model in road transport systems

    Science.gov (United States)

    Sakhapov, R. L.; Nikolaeva, R. V.; Gatiyatullin, M. H.; Makhmutov, M. M.

    2016-08-01

    The article presents the results of a study of road safety indicators that influence the development and operation of the transport system. Road safety is considered as a continuous process of risk management. Authors constructed a model that relates the social risks of a major road safety indicator - the level of motorization. The model gives a fairly accurate assessment of the level of social risk for any given level of motorization. Authors calculated the dependence of the level of socio-economic costs of accidents and injured people in them. The applicability of the concept of socio-economic damage is caused by the presence of a linear relationship between the natural and economic indicators damage from accidents. The optimization of social risk is reduced to finding the extremum of the objective function that characterizes the economic effect of the implementation of measures to improve safety. The calculations make it possible to maximize the net present value, depending on the costs of improving road safety, taking into account socio-economic damage caused by accidents. The proposed econometric models make it possible to quantify the efficiency of the transportation system, allow to simulate the change in road safety indicators.

  13. Innovative technology summary report: Transportable vitrification system

    International Nuclear Information System (INIS)

    1998-09-01

    At the end of the cold war, many of the Department of Energy's (DOE's) major nuclear weapons facilities refocused their efforts on finding technically sound, economic, regulatory compliant, and stakeholder acceptable treatment solutions for the legacy of mixed wastes they had produced. In particular, an advanced stabilization process that could effectively treat the large volumes of settling pond and treatment sludges was needed. Based on this need, DOE and its contractors initiated in 1993 the EM-50 sponsored development effort required to produce a deployable mixed waste vitrification system. As a consequence, the Transportable Vitrification System (TVS) effort was undertaken with the primary requirement to develop and demonstrate the technology and associated facility to effectively vitrify, for compliant disposal, the applicable mixed waste sludges and solids across the various DOE complex sites. After 4 years of development testing with both crucible and pilot-scale melters, the TVS facility was constructed by Envitco, evaluated and demonstrated with surrogates, and then successfully transported to the ORNL ETTP site and demonstrated with actual mixed wastes in the fall of 1997. This paper describes the technology, its performance, the technology applicability and alternatives, cost, regulatory and policy issues, and lessons learned

  14. Road Transportable Analytical Laboratory (RTAL) system

    International Nuclear Information System (INIS)

    Finger, S.M.

    1995-01-01

    U.S. Department of Energy (DOE) facilities around the country have, over the years, become contaminated with radionuclides and a range of organic and inorganic wastes. Many of the DOE sites encompass large land areas and were originally sited in relatively unpopulated regions of the country to minimize risk to surrounding populations. In addition, wastes were sometimes stored underground at the sites in 55-gallon drums, wood boxes or other containers until final disposal methods could be determined. Over the years, these containers have deteriorated, releasing contaminants into the surrounding environment. This contamination has spread, in some cases polluting extensive areas. The DOE would benefit greatly if it had reliable, road transportable, fully independent laboratory systems that could perform on-site the full range of analyses required. The goal of the Road Transportable Analytical Laboratory (RTAL) project is the development and demonstration of a system to meet the unique needs of the DOE for rapid, accurate analysis of a wide variety of hazardous and radioactive contaminants in soils, ground water and surface waters. This document describes the requirements for such a laboratory

  15. A multidimensonal Examination of Prefomences of the Future advanced Transport Systems: The ETT (Evacuated Tube Transport) TRM (Transrapid MAGLEV) System

    NARCIS (Netherlands)

    Janic, M.

    2016-01-01

    Multidimensional examination of performances of the future advanced ETT Evacuated Tube Transport) system operated by TRM (TransRapidMaglev); assessment of the ETT TRM system contribution to sustainability of the future transport sector through its completion with APT (Air Passenger Transport) system

  16. TRANSPORT PROPERTIES OF THE STRONGLY CORRELATED SYSTEMS

    Directory of Open Access Journals (Sweden)

    T.Domanski

    2004-01-01

    Full Text Available The transport properties of various systems are studied here in the context of three different models. These are: - the disordered Hubbard model applicable to correlated binary alloys with a general disorder, - the Anderson model used in describing the Kondo physics of a quantum dot connected to the external superconducting leads, and - the Ranninger-Robaszkiewicz model applied to the study of optical properties of the system with preformed electron pairs above the temperature of transition to the superconducting state. We calculate the density of states, specific heat, the Wilson ratio and conductivity of the correlated binary alloy with off-diagonal disorder. We investigate the conditions under which the Kondo peak appears in the density of states and in the conductance of a dot coupled to the external superconducting leads. We analyze the effect of the pseudogap on the optical spectra in the high temperature superconductors described by the boson-fermion model.

  17. Sensor system for fuel transport vehicle

    Science.gov (United States)

    Earl, Dennis Duncan; McIntyre, Timothy J.; West, David L.

    2016-03-22

    An exemplary sensor system for a fuel transport vehicle can comprise a fuel marker sensor positioned between a fuel storage chamber of the vehicle and an access valve for the fuel storage chamber of the vehicle. The fuel marker sensor can be configured to measure one or more characteristics of one or more fuel markers present in the fuel adjacent the sensor, such as when the marked fuel is unloaded at a retail station. The one or more characteristics can comprise concentration and/or identity of the one or more fuel markers in the fuel. Based on the measured characteristics of the one or more fuel markers, the sensor system can identify the fuel and/or can determine whether the fuel has been adulterated after the marked fuel was last measured, such as when the marked fuel was loaded into the vehicle.

  18. Quantum Transport in Strongly Correlated Systems

    DEFF Research Database (Denmark)

    Bohr, Dan

    2007-01-01

    the density matrix renormalization group (DMRG) method. We present two DMRG setups for calculating the linear conductance of strongly correlated nanostructures in the infinitesimal source-drain voltage regime. The first setup describes the leads by modified real-space tight-binding chains, whereas the second....... Thus both coherence and correlation effects are important in this model, and the methods applied should be able to handle both these effects rigorously. We present the DMRG setup for this model and benchmark against existing Greens function results for the model. Then we present initial DMRG results...... screening plays a much less significant role than in bulk systems due to the reduced size of the objects, therefore making it necessary to consider the importance of correlations between electrons. The work presented in this thesis deals with quantum transport through strongly correlated systems using...

  19. Road Transportable Analytical Laboratory system. Phase 1

    Energy Technology Data Exchange (ETDEWEB)

    Finger, S.M.; Keith, V.F.; Spertzel, R.O.; De Avila, J.C.; O`Donnell, M.; Vann, R.L.

    1993-09-01

    This developmental effort clearly shows that a Road Transportable Analytical Laboratory System is a worthwhile and achievable goal. The RTAL is designed to fully analyze (radioanalytes, and organic and inorganic chemical analytes) 20 samples per day at the highest levels of quality assurance and quality control. It dramatically reduces the turnaround time for environmental sample analysis from 45 days (at a central commercial laboratory) to 1 day. At the same time each RTAL system will save the DOE over $12 million per year in sample analysis costs compared to the costs at a central commercial laboratory. If RTAL systems were used at the eight largest DOE facilities (at Hanford, Savannah River, Fernald, Oak Ridge, Idaho, Rocky Flats, Los Alamos, and the Nevada Test Site), the annual savings would be $96,589,000. The DOE`s internal study of sample analysis needs projects 130,000 environmental samples requiring analysis in FY 1994, clearly supporting the need for the RTAL system. The cost and time savings achievable with the RTAL system will accelerate and improve the efficiency of cleanup and remediation operations throughout the DOE complex.

  20. Integral Transportation Systems in Military Transport Aircraft Supply

    Directory of Open Access Journals (Sweden)

    Dražen Kovačević

    2012-10-01

    Full Text Available Supply of goods, equipment and soldiers by militwy transportaircraft can serve as a support to airborne landing operation,support to encircled forces, and support to forces leadinga gue1rilla war. Transport aircraft are designed in such a wayas to be able to cany containers, pallets, most of land vehiclesand helicopters. Militwy transport aircraft can be grouped intothose that were originally designed for military transp01t andthose that are modified civilian aircraft and helicopters. Supplypallets can be wooden, metal, can be airdropped in "taxiing","low-flight", and can also be fitted with a parachute or"retrorocket" for reducing the ground impact. Pallets canamong other things carry liquids, heavy combat and ca1rier vehicles,artillery and rocket weapons and valious containers.Pallets are usually pe1manently deformed at ground impact.Nowadays, high precision of airdrop has been achieved. Containersare used to carry various equipment, food, fue~ weapons,ammunition etc. It is to be expected that the containers,wmoured combat and other vehicles will be redesigned so asto provide more efficient transport and fast a!Tangement ofhigh-mobility units, whereas the form of the future militarytransport aircraft will not undergo substantial changes. By adjustingand standardising the transporlation vehicles, integraltransportation means and cwgo, the overall combat efficiencywill be increased, the a~rangement time especially shortenedand the air supply safety increased.

  1. Vertical Transport by Coastal Mesoscale Convective Systems

    Science.gov (United States)

    Lombardo, K.; Kading, T.

    2016-12-01

    This work is part of an ongoing investigation of coastal mesoscale convective systems (MCSs), including changes in vertical transport of boundary layer air by storms moving from inland to offshore. The density of a storm's cold pool versus that of the offshore marine atmospheric boundary layer (MABL), in part, determines the ability of the storm to successfully cross the coast, the mechanism driving storm propagation, and the ability of the storm to lift air from the boundary layer aloft. The ability of an MCS to overturn boundary layer air can be especially important over the eastern US seaboard, where warm season coastal MCSs are relatively common and where large coastal population centers generate concentrated regions of pollution. Recent work numerically simulating idealized MCSs in a coastal environment has provided some insight into the physical mechanisms governing MCS coastal crossing success and the impact on vertical transport of boundary layer air. Storms are simulated using a cloud resolving model initialized with atmospheric conditions representative of a Mid-Atlantic environment. Simulations are run in 2-D at 250 m horizontal resolution with a vertical resolution stretched from 100 m in the boundary layer to 250 m aloft. The left half of the 800 km domain is configured to represent land, while the right half is assigned as water. Sensitivity experiments are conducted to quantify the influence of varying MABL structure on MCS coastal crossing success and air transport, with MABL values representative of those observed over the western Mid-Atlantic during warm season. Preliminary results indicate that when the density of the cold pool is much greater than the MABL, the storm successfully crosses the coastline, with lifting of surface parcels, which ascend through the troposphere. When the density of the cold pool is similar to that of the MABL, parcels within the MABL remain at low levels, though parcels above the MABL ascend through the troposphere.

  2. Conceptual Design Studies of the KSTAR Bay-Nm Cassette and Thomson Scattering Optics

    International Nuclear Information System (INIS)

    Feder, R.; Ellis, R.; Johnson, D.; Park, H.; Lee, H.G.

    2005-01-01

    A Multi-Channel Thomson Scattering System viewing the edge and core of the KSTAR plasma will be installed at the mid-plane port Bay-N. An engineering design study was undertaken at PPPL in collaboration with the Korea Basic Science Institute (KBSI) to determine the optimal optics and cassette design. Design criteria included environmental, mechanical and optical factors. All of the optical design options have common design features; the Thomson Scattering laser, an in-vacuum shutter, a quartz heat shield and primary vacuum window, a set of optical elements and a fiber optic bundle. Neutron radiation damage was a major factor in the choice of competing lens-based and mirror-based optical designs. Both the mirror based design and the lens design are constrained by physical limits of the Bay-N cassette and interference with the Bay-N micro-wave launcher. The cassette will contain the optics and a rail system for maintenance of the optics

  3. Propagating fronts in reaction-transport systems with memory

    Energy Technology Data Exchange (ETDEWEB)

    Yadav, A. [Department of Chemistry, Southern Methodist University, Dallas, TX 75275-0314 (United States)], E-mail: ayadav1@lsu.edu; Fedotov, Sergei [School of Mathematics, University of Manchester, Manchester M60 1DQ (United Kingdom)], E-mail: sergei.fedotov@manchester.ac.uk; Mendez, Vicenc [Grup de Fisica Estadistica, Departament de Fisica, Universitat Autonoma de Barcelona, E-08193 Bellaterra (Spain)], E-mail: vicenc.mendez@uab.es; Horsthemke, Werner [Department of Chemistry, Southern Methodist University, Dallas, TX 75275-0314 (United States)], E-mail: whorsthe@smu.edu

    2007-11-26

    In reaction-transport systems with non-standard diffusion, the memory of the transport causes a coupling of reactions and transport. We investigate the effect of this coupling for systems with Fisher-type kinetics and obtain a general analytical expression for the front speed. We apply our results to the specific case of subdiffusion.

  4. Sustainable Transportation Systems Research Group: Ongoing and Past Activities

    OpenAIRE

    Gkritza, Konstantina "Nadia"; Hurtado, Davis Chacon; Gkartzonikas, Christos; Ke, Yue; Losada, Lisa L

    2017-01-01

    This presentation describes the ongoing and past activities of the Sustainable Transportation Systems Research (STSR) group at Purdue University (https://engineering.purdue.edu/STSRG). The STSR group aims to achieve green, safe, efficient, and equitable transportation systems by studying and modeling transportation externalities, using state of the art statistical, econometric, and economic analysis tools.

  5. 77 FR 57640 - Intelligent Transportation Systems Program Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2012-09-18

    ... DEPARTMENT OF TRANSPORTATION Intelligent Transportation Systems Program Advisory Committee; Notice.... Department of Transportation. ACTION: Notice. The Intelligent Transportation Systems (ITS) Program Advisory... intelligent transportation systems. Through its sponsor, the ITS Joint Program Office, the ITS PAC makes...

  6. 78 FR 64048 - Intelligent Transportation Systems Program Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2013-10-25

    ... DEPARTMENT OF TRANSPORTATION Intelligent Transportation Systems Program Advisory Committee; Notice.... Department of Transportation. ACTION: Notice. The Intelligent Transportation Systems (ITS) Program Advisory... implementation of intelligent transportation systems. Through its sponsor, the ITS Joint Program Office (JPO...

  7. 77 FR 20872 - Intelligent Transportation Systems Program Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2012-04-06

    ... DEPARTMENT OF TRANSPORTATION Intelligent Transportation Systems Program Advisory Committee; Notice.... Department of Transportation. ACTION: Notice. The Intelligent Transportation Systems (ITS) Program Advisory..., development, and implementation of intelligent transportation systems. Through its sponsor, the ITS Joint...

  8. 77 FR 51845 - Intelligent Transportation Systems Program Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2012-08-27

    ... DEPARTMENT OF TRANSPORTATION Intelligent Transportation Systems Program Advisory Committee; Notice.... Department of Transportation. ACTION: Notice. The Intelligent Transportation Systems (ITS) Program Advisory..., development, and implementation of intelligent transportation systems. Through its sponsor, the ITS Joint...

  9. 77 FR 26067 - Intelligent Transportation Systems Program Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2012-05-02

    ... DEPARTMENT OF TRANSPORTATION Intelligent Transportation Systems Program Advisory Committee; Notice.... Department of Transportation. ACTION: Notice. The Intelligent Transportation Systems (ITS) Program Advisory... of intelligent transportation systems. Through its sponsor, the ITS Joint Program Office, the ITS PAC...

  10. Picosecond excitation transport in disordered systems

    International Nuclear Information System (INIS)

    Hart, D.E.

    1987-11-01

    Time-resolved fluorescence decay profiles are used to study excitation transport in 2- and 3-dimensional disordered systems. Time-correlated single photon counting detection is used to collect the fluorescence depolarization data. The high signal-to-noise ratios afforded by this technique makes it possible to critically examine current theories of excitation transport. Care has been taken to eliminate or account for the experimental artifacts common to this type of study. Solutions of 3,3'-diethyloxadicarbocyanine iodide (DODCI) in glycerol serve as a radomly distributed array of energy donors in 3-dimensions. A very thin sample cell (/approximately/ 2 μm) is used to minimize the effects of fluorescence self-absorption on the decay kinetics. Evidence of a dynamic shift of the fluorescence spectrum of DODCI in glycerol due to solvent reorganization is presented. The effects of excitation trapping on the decay profiles is minimized in the data analysis procedure. The 3-body theory of Gochanour, Andersen, and Fayer (GAF) and the far less complex 2-particle analytic theory of Huber, Hamilton, and Barnett yield indistinguishable fits to the data over the wide dynamic range of concentrations and decay times studied

  11. Wireless Battery Management System of Electric Transport

    Science.gov (United States)

    Rahman, Ataur; Rahman, Mizanur; Rashid, Mahbubur

    2017-11-01

    Electric vehicles (EVs) are being developed and considered as the future transportation to reduce emission of toxic gas, cost and weight. The battery pack is one of the main crucial parts of the electric vehicle. The power optimization of the battery pack has been maintained by developing a two phase evaporative thermal management system which operation has been controlled by using a wireless battery management system. A large number of individual cells in a battery pack have many wire terminations that are liable for safety failure. To reduce the wiring problem, a wireless battery management system based on ZigBee communication protocol and point-to-point wireless topology has been presented. Microcontrollers and wireless modules are employed to process the information from several sensors (voltage, temperature and SOC) and transmit to the display devices respectively. The WBMS multistage charge balancing system offering more effective and efficient responses for several numbers of series connected battery cells. The concept of double tier switched capacitor converter and resonant switched capacitor converter is used for reducing the charge balancing time of the cells. The balancing result for 2 cells and 16 cells are improved by 15.12% and 25.3% respectively. The balancing results are poised to become better when the battery cells are increased.

  12. Establishing an intelligent transportation systems (ITS) lab at LTRC.

    Science.gov (United States)

    2008-09-30

    The primary goal of this research project is to lay the foundation for establishing a state-of-the-art Intelligent Transportation Systems (ITS) : lab at the Louisiana Transportation Research Center (LTRC), where data will be collected, analyzed, and ...

  13. Modelling the Global Transportation Systems for the Hydrogen Economy

    Energy Technology Data Exchange (ETDEWEB)

    Krzyzanowski, D.A.; Kypreos, S.

    2004-03-01

    A modelling analysis of the transportation system is described, focused on the market penetration of different transportation technologies (including Learning-by-Doing) until the year 2050. A general outline of the work and first preliminary results are presented. (author)

  14. Costs and benefits of MDOT intelligent transportation system deployments.

    Science.gov (United States)

    2015-07-01

    This report analyses costs and benefits of Intelligent Transportation Systems (ITS) deployed by : the Michigan Department of Transportation (MDOT). MDOT ITS focuses on traffic incident : management and also provide Freeway Courtesy Patrol services. A...

  15. Sensor network design for multimodal freight transportation systems.

    Science.gov (United States)

    2009-10-15

    The agricultural and manufacturing industries in the US Midwest region rely heavily on the efficiency of freight transportation systems. While the growth of freight movement far outpaces that of the transportation infrastructure, ensuring the efficie...

  16. Functional requirements for a comprehensive transportation location referencing system

    Science.gov (United States)

    2000-06-01

    Transportation agencies manage data that is referenced in one, two, three, and four dimensions. Location referencing system (LRS) data models vary across transportation agencies and often within organizations as well. This has resulted in failed atte...

  17. Advanced transport systems analysis, modeling, and evaluation of performances

    CERN Document Server

    Janić, Milan

    2014-01-01

    This book provides a systematic analysis, modeling and evaluation of the performance of advanced transport systems. It offers an innovative approach by presenting a multidimensional examination of the performance of advanced transport systems and transport modes, useful for both theoretical and practical purposes. Advanced transport systems for the twenty-first century are characterized by the superiority of one or several of their infrastructural, technical/technological, operational, economic, environmental, social, and policy performances as compared to their conventional counterparts. The advanced transport systems considered include: Bus Rapid Transit (BRT) and Personal Rapid Transit (PRT) systems in urban area(s), electric and fuel cell passenger cars, high speed tilting trains, High Speed Rail (HSR), Trans Rapid Maglev (TRM), Evacuated Tube Transport system (ETT), advanced commercial subsonic and Supersonic Transport Aircraft (STA), conventionally- and Liquid Hydrogen (LH2)-fuelled commercial air trans...

  18. Biking and Walking: The Position of Non-Motorised Transport Modes in Transport Systems

    NARCIS (Netherlands)

    Rietveld, Piet

    2001-01-01

    Long run developments such as income growth and urban sprawl lead one to expect a continuous decline of thecontribution of non-motorised transport modes to the performance of transport systems. In terms of the total number of trips, non-motorised transport modes have retained high shares, however.

  19. Modelling the transport system in China and evaluating the current strategies towards the sustainable transport development

    DEFF Research Database (Denmark)

    Liu, W.; Lund, H.; Mathiesen, B.V.

    2013-01-01

    in China. With this purpose in mind, a Chinese transport model has been created and three current transport strategies which are high speed railway (HSR), urban rail transit (URT) and electric vehicle (EV) were evaluated together with a reference transport system in 2020. As conservative results, 13...

  20. The characteristics of Fugi IP Cassette Type PII and application for radiation oncology quality assurance tests and portal imaging

    International Nuclear Information System (INIS)

    Soh, H.S.; Ung, N.M.; Ng, K.H.

    2008-01-01

    Full text: The advancement of digital imaging has prompted more medical institutions to go filmless. The computed radiography (CR) system is becoming an important tool not only in diagnostic imaging, but also in radiation oncology. A new CR system that was specially designed for the use in radiation oncology. Fuji IP cassette type PII has been introduced to the market in the middle of year 2006. This project aimed to study some basic physical characteristics of this new type of cassette and explore its application for performing quality assurance (QA) tests and portal imaging in radiotherapy. All the images were read by FCR 5000 Plus reader. The image was found to reach its saturation value of 1023 (due to the image was stored in 10 bits data) by depending on the sensitivity value being adjusted. The uniformity test gave the result of 0.12%. The cassette was used to perform the QA tests which were previously performed using film. All the results met the specification as stated in AAPM Task Group 40. The comparison for the portal images of Portal Vision contrast-detail phantom showed that the spatial resolution of the images obtained by CR system (Fujifilm Co.. Ltd.. Tokyo. Japan) were better than the EPID (Varian Medical Systems. Inc.. Palo Alto. USA) and film system (Eastman Kodak Co.. New York. USA). The IP cassette type PII was found to be suitable as an alternative QA test tool and portal imaging in radiotherapy.

  1. Evaluation of D-1 tape and cassette characteristics: Moisture content of Sony and Ampex D-1 tapes when delivered

    Science.gov (United States)

    Ashton, Gary

    Commercial D-1 cassette tapes and their associated recorders were designed to operate in broadcast studios and record in accordance with the International Radio Consultative Committee (CCIR) 607 digital video standards. The D-1 recorder resulted in the Society of Motion Picture and Television Engineers (SMPTE) standards 224 to 228 and is the first digital video recorder to be standardized for the broadcast industry. The D-1 cassette and associated media are currently marketed for broadcast use. The recorder was redesigned for data applications and is in the early stages of being evaluated. The digital data formats used are specified in MIL-STD-2179 and the American National Standards Institute (ANSI) X3.175-190 standard. In early 1990, the National Media Laboratory (NML) was asked to study the effects of time, temperature, and relative humidity on commercial D-1 cassettes. The environmental range to be studied was the one selected for the Advanced Tactical Air Reconnaissance System (ATARS) program. Several discussions between NML personnel, ATARS representatives, recorder contractors, and other interested parties were held to decide upon the experimental plan to be implemented. Review meetings were held periodically during the course of the experiment. The experiments were designed to determine the dimensional stability of the media and cassette since this is one of the major limiting factors of helical recorders when the media or recorders are subjected to non-broadcasting environments. Measurements were also made to characterize each sample of cassettes to give preliminary information on which purchase specifications could be developed. The actual tests performed on the cassettes and media before and after aging fall into the general categories listed.

  2. Monju secondary heat transport system sodium leak

    International Nuclear Information System (INIS)

    Suzuki, Takeo; Hiroi, Hiroshi; Usami, Shin; Iwata, Koji.

    1996-01-01

    On December 8, 1995, the sodium leakage from the secondary heat transport system (SHTS) occurred in the piping room of the reactor auxiliary building in Monju. The secondary sodium leaked through a temperature sensor, due to the breakaway of the tip of the well tube of the sensor installed near the outlet of the intermediate heat exchanger (IHX) in the C loop of SHTS. The reactor core remained cooled and thus, from the viewpoint of radiological hazards, the safety of the reactor was secured. There were no adverse effects for operating personnel or the surrounding environment. The cause of the well tube failure is considered to result from high cycle fatigue due to flow induced vibrations. Delay in draining the sodium from the leaking loop increased the consequential effects from sodium combustion products. (author)

  3. The safe road transport system approach

    Directory of Open Access Journals (Sweden)

    Vollpracht Hans-Joachim

    2016-07-01

    Full Text Available More than 1,24 million people die each year on the worlds roads and between 20 to 50 million suffer from nonfatal injuries. The UN Road Safety Collaboration Meetings under the leadership of WHO developed the Programme for the Decade of Actions for road safety taking nations into the responsibility of improving their accident figures by the five pillars of a national Road Safety Policy, safer Roads, safer Vehicles, safer Road Users and Post Crash Care. It is this Safe System Approach that takes into consideration the land use, infrastructure and transport planning, road user’s abilities and limitations and the close cooperation of all governmental and none governmental stakeholders involved.

  4. Neratinib Reverses ATP-Binding Cassette B1-Mediated Chemotherapeutic Drug Resistance In Vitro, In Vivo, and Ex Vivo

    OpenAIRE

    Zhao, Xiao-qin; Xie, Jing-dun; Chen, Xing-gui; Sim, Hong May; Zhang, Xu; Liang, Yong-ju; Singh, Satyakam; Talele, Tanaji T.; Sun, Yueli; Ambudkar, Suresh V.; Chen, Zhe-Sheng; Fu, Li-wu

    2012-01-01

    Neratinib, an irreversible inhibitor of epidermal growth factor receptor and human epidermal receptor 2, is in phase III clinical trials for patients with human epidermal receptor 2-positive, locally advanced or metastatic breast cancer. The objective of this study was to explore the ability of neratinib to reverse tumor multidrug resistance attributable to overexpression of ATP-binding cassette (ABC) transporters. Our results showed that neratinib remarkably enhanced the sensitivity of ABCB1...

  5. ASTRA - an automatic system for transport analysis in a tokamak

    International Nuclear Information System (INIS)

    Pereverzev, G.V.; Yushmanov, P.N.; Dnestrovskii, A.Yu.; Polevoi, A.R.; Tarasjan, K.N.; Zakharov, L.E.

    1991-08-01

    The set of codes described here - ASTRA (Automatic System of Transport Analysis) - is a flexible and effective tool for the study of transport mechanisms in reactor-oriented facilities of the tokamak type. Flexibility is provided within the ASTRA system by a wide choice of standard relationships, functions and subroutines representing various transport coefficients, methods of auxiliary heating and other physical processes in the tokamak plasma, as well as by the possibility of pre-setting transport equations and variables for data output in a simple and conseptually transparent form. The transport code produced by the ASTRA system provides an adequate representation of the discharges for present experimental conditions. (orig.)

  6. Blanket maintenance by remote means using the cassette blanket approach

    International Nuclear Information System (INIS)

    Werner, R.W.

    1978-01-01

    Induced radioactivity in the blanket and other parts of a fusion reactor close to the plasma zone will dictate remote assembly, disassembly, and maintenance procedures. Time will be of the essence in these procedures. They must be practicable and certain. This paper discusses the reduction of a complicated Tokamak reactor to a simpler assembly via the use of a vacuum building in which to house the reactor and the introduction in this new model of cassette blanket modules. The cassettes significantly simplify remote handling

  7. An Agent-Based Approach for a Smart Transport System

    Directory of Open Access Journals (Sweden)

    Cristian PEÑARANDA

    2016-11-01

    Full Text Available This paper presents a proposal for a Smart Transport System which is an application that facilitates the interconnection between people (citizens, tourists and transport providers (Bus, metro, trains, trams, defining the services that everyone can request or offer. The system has been defined as a virtual organization where agents (representing actors of the transport system can enter or leave into the system consuming or offering services. Due to the fact that modern urban public transport is increasingly an important service used by citizens in current cities, the proposed system will improve the use of resources while also ensuring time flexible mobility solutions for citizens.

  8. Numerical Modelling Approaches for Sediment Transport in Sewer Systems

    DEFF Research Database (Denmark)

    Mark, Ole

    A study of the sediment transport processes in sewers has been carried out. Based on this study a mathematical modelling system has been developed to describe the transport processes of sediments and dissolved matter in sewer systems. The modelling system consists of three sub-models which...... constitute the basic modelling system necessary to give a discription of the most dominant physical transport processes concerning particles and dissolved matter in sewer systems: A surface model. An advection-dispersion model. A sediment transport model....

  9. Public acceptance of radioactive waste transportation systems

    International Nuclear Information System (INIS)

    Gablin, K.A.

    1978-01-01

    As the thoughts of the country concentrate on the problems of transportation of waste through high traffic urban areas, the problem of how to deal directly and honestly with the public takes on greater significance in the nuclear industry. Non-technical aspects of the methods of transportation, especially by railroad and highway, enter into the total scheme of moving radioactive waste from both weapon and nuclear power plant sources to final processing and disposal. Factors such as shape, color, size, familiarity, and industrial designing are necessary ingredients that take on equal or more significance that the designing of containers to survive the hypothetical accident conditions of the present, or even of the future. Protective Packaging, Inc. has been a leader in the presentation of containers to the private and public sector of the nuclear industry. The products have undergone very open testing, in public, with both invited and uninvited witnesses. In those experiences, dating back to 1969, the problems of public acceptance will be related between the technical problems and the associated social and political problems that relate to container acceptance by the public in today's world. Proven experience data, relative to the safety of the present day systems will be discussed, as well as methods of improving the image in the future. Review will also be given to the effort by industry to discuss the proven record with parties outside the nuclear industry, i.e., individuals and pressure groups that are diametrically opposed to review the facts relative to safety as opposed to other, but more traditional industries

  10. Education in Transportation Systems Planning: Highway Research Record No. 462.

    Science.gov (United States)

    National Academy of Sciences - National Research Council, Washington, DC. Transportation Research Board.

    The papers contained in the issue of Highway Research Record focus on current and emerging patterns of education and training related to transportation systems planning. The five papers are: Transportation Centers and Other Mechanisms to Encourage Interdisciplinary Research and Training Efforts in Transportation (Frederick J. Wegmann and Edward A.…

  11. 78 FR 16030 - Intelligent Transportation Systems Program Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2013-03-13

    ... DEPARTMENT OF TRANSPORTATION Intelligent Transportation Systems Program Advisory Committee; Notice.... Department of Transportation. ACTION: Notice. The Intelligent Transportation Systems (ITS) Program Advisory... Transportation on all matters relating to the study, development, and implementation of intelligent...

  12. The SIMPSONS project: An integrated Mars transportation system

    Science.gov (United States)

    Kaplan, Matthew; Carlson, Eric; Bradfute, Sherie; Allen, Kent; Duvergne, Francois; Hernandez, Bert; Le, David; Nguyen, Quan; Thornhill, Brett

    In response to the Request for Proposal (RFP) for an integrated transportation system network for an advanced Martian base, Frontier Transportation Systems (FTS) presents the results of the SIMPSONS project (Systems Integration for Mars Planetary Surface Operations Networks). The following topics are included: the project background, vehicle design, future work, conclusions, management status, and cost breakdown. The project focuses solely on the surface-to-surface transportation at an advanced Martian base.

  13. Mixed-μ magnetic levitation for advanced ground transport system

    International Nuclear Information System (INIS)

    Russell, F.M.

    1977-12-01

    The possibility of applying the mixed-μ principle for magnetic levitation to ground transport systems is examined. The system is developed specifically for suspension and useful lift to passive weight ratios exceeding 8:1 have been calculated. Application to a hybrid system where conventional wheel drive is used in conjunction with magnetic levitation is explained for urban transport. (author)

  14. Environmental analyses of land transportation systems in The Netherlands

    NARCIS (Netherlands)

    Bouwman, Mirjan E.; Moll, Henri C.

    Environmental analyses of the impact of transportation systems on the environment from the cradle to the grave are rare. This article makes a comparison of various Dutch passenger transportation systems by studying their complete life-cycle energy use. Moreover, systems are compared according to

  15. Transportation system requirements document. Revision 1 DCN01. Supplement

    International Nuclear Information System (INIS)

    1995-05-01

    The original Transportation System Requirements Document described the functions to be performed by and the technical requirements for the Transportation System to transport spent nuclear fuel (SNF) and high-level radioactive waste (HLW) from Purchaser and Producer sites to a Civilian Radioactive Waste Management System (CRWMS) site, and between CRWMS sites. The purpose of that document was to define the system-level requirements. These requirements include design and operations requirements to the extent they impact on the development of the physical segments of Transportation. The document also presented an overall description of Transportation, its functions, its segments, and the requirements allocated to the segments and the system-level interfaces with Transportation. This revision of the document contains only the pages that have been modified

  16. The Exchange Value Embedded in a Transport System

    International Nuclear Information System (INIS)

    Xia Qinglan; Xu Shaofeng

    2010-01-01

    This paper shows that a well designed transport system has an embedded exchange value by serving as a market for potential exchange between consumers. Under suitable conditions, one can improve the welfare of consumers in the system simply by allowing some exchange of goods between consumers during transportation without incurring additional transportation cost. We propose an explicit valuation formula to measure this exchange value for a given compatible transport system. This value is always nonnegative and bounded from above. Criteria based on transport structures, preferences and prices are provided to determine the existence of a positive exchange value. Finally, we study a new optimal transport problem with an objective taking into account of both transportation cost and exchange value.

  17. Stochasticity and transport in Hamiltonian systems

    International Nuclear Information System (INIS)

    MacKay, R.S.; Meiss, J.D.; Percival, I.C.

    1984-01-01

    The theory of transport in nonlinear dynamics is developed in terms of ''leaky'' barriers which remain when invariant tori are destroyed. A critical exponent for transport times across destroyed tori is obtained which explains numerical results of Chirikov. The combined effects of many destroyed tori lead to power-law decay of correlations observed in many computations. (author)

  18. Further development of the cassette-based pYC plasmid system by incorporation of the dominant hph, nat and AUR1-C gene markers and the lacZ reporter system

    DEFF Research Database (Denmark)

    Hansen, J.; Felding, T.; Johannesen, P.F.

    2003-01-01

    to the use of G418 resistance. We found the markers to be of use not only in standard laboratory strains of Saccharomyces cerevisiae but also in an industrial strain of S. carlsbergensis (syn. of S. pastorianus) brewing yeast as well as in Saccharomyces kluyveri. As the pYC system contains means of counter...

  19. Evaluation of an Audio Cassette Tape Lecture Course

    Science.gov (United States)

    Blank, Jerome W.

    1975-01-01

    An audio-cassette continuing education course (Selected Topics in Pharmacology) from Extension Services in Pharmacy at the University of Wisconsin was offered to a selected test market of pharmacists and evaluated using a pre-, post-test design. Results showed significant increase in cognitive knowledge and strong approval of students. (JT)

  20. Cassette - Foro. Un sistema de comunicación participatoria

    Directory of Open Access Journals (Sweden)

    Mario Kaplún

    2015-05-01

    Full Text Available El Cassette-Foro es un modelo de comunicación para la promoción y educación de adultos, puesto al servicio de organizaciones de desarrollo comunitario -rurales y urbanas-, centrales cooperativas, centros de educación pública, etc. El método es grupal y bidireccional. Mediante el intercambio de mensaJes grabados en cassettes, permite entablar a distancia un diálogo entre los miembros de base de la organización y el núcleo dirigente de la misma y/o entre los grupos de base entre sí. El modelo combina la comunicación colectiva con la interpersonal: mensajes colectivos grabados en cassettes, audición del mensaje por parte de cada grupo, discusión del mismo y respuesta del grupo grabada en la otra pista del cassette, que retorna al centro emisor-receptor.

  1. p21-ras effector domain mutants constructed by "cassette" mutagenesis

    DEFF Research Database (Denmark)

    Stone, J C; Vass, W C; Willumsen, B M

    1988-01-01

    A series of mutations encoding single-amino-acid substitutions within the v-rasH effector domain were constructed, and the ability of the mutants to induce focal transformation of NIH 3T3 cells was studied. The mutations, which spanned codons 32 to 40, were made by a "cassette" mutagenesis...

  2. The Real World French Cassette Program. Script Book.

    Science.gov (United States)

    Sternburg, Sheldon G.; Sammarco, Anthony M., Jr.

    This dual cassette package, accompanied by a script book, is designed to give students listening practice in French, particularly for regional differences of pronunciation and for variety in idiomatic constructions. The program may be integrated with texts used in intermediate and advanced levels of instruction. The announcements, jingles, and…

  3. The Real World Spanish Cassette Program. Script Book.

    Science.gov (United States)

    Sternburg, Sheldon G.

    This dual cassette program, accompanied by a script book, is designed to give students listening practice in Spanish, particularly for regional differences of pronunciation and for variety in idiomatic construction. The program may be integrated with texts used in intermediate and advanced levels of instruction. The announcements, jingles, and…

  4. Indicator system for the environmental assessment of energy transport systems

    International Nuclear Information System (INIS)

    Knoepfel, I.

    1995-01-01

    The aim of this dissertation is to define a consistent set of indicators for the environmental assessment of different energy transport systems: high-voltage alternating and direct current transmission lines, electric cables, pipelines for gas and oil, inland waterway, road and rail transportation, according to state-of-the-art technologies. The indicator system is used for comparative analysis and identification of environmental hot-spots of the different systems. The environmental performance of power plants close to production or unloading terminals with subsequent power transmission and the transport of fossil fuels with power production close to the end-users is compared. Quantitative indicators are defined for different impact categories: fossil energy depletion, impacts from emissions, land use, noise impacts and visibility. A further aggregation of the different indicators to obtain a universal environmental score was not envisaged. It was not possible to define a quantitative indicator for possible electric and magnetic field effects because of insufficient knowledge of the involved dose-response metrics. The proposed indicators quantify dose-response relationships also below emission or immission limits imposed by law, which was one of the main requirements in this work. By reducing all information to an equivalent impacted area, a high level of consistency was achieved for land use, noise impacts and visibility indicators. Other indicators refer to the energy content of fossil resources and to equivalent emissions of reference substances. The calculation of an equivalent impacted area was not considered an efficient approach in these cases. The performance of the proposed indicator system and its applicability to infrastructure and regional planning is tested in two practical examples. (author) figs., tabs., refs

  5. Sustainable Transport in Romania vs. European Union. Analysis of Road Transport System from the Sustainable Transport Perspective

    Directory of Open Access Journals (Sweden)

    Clitan Andrei - Florin

    2014-12-01

    Full Text Available Sustainability is a term used more often lately, based on three factors: social, economic, and environmental. Sustainable transport systems increase social cohesion, reduce environmental problems and help create a more efficient economy. Sustainable transport consists in a complex system that is designed to ensure mobility needs of present generations without damaging the environment and health factors. By improving energy and material consumption, it must be capable to satisfy in optimum conditions the need for mobility for future generations. The current transportation system has not a character of sustainability.

  6. INFORMATION SYSTEMS APPLIED TO TRANSPORT IMPROVEMENT

    Directory of Open Access Journals (Sweden)

    JULIAN ANDRES ZAPATA CORTES

    2013-01-01

    Full Text Available El transporte es uno de los elementos más relevantes para la competitividad de las empresas y las ciudades, pues un sistema de transporte inadecuado genera altos costos y bajos niveles de servicio al cliente, lo que fi nalmente produce un impacto económico para ambas partes. Este artículo presenta una revisión de herramientas tecnológicas que hacen parte de los sistemas de transporte inteligentes (ITS, sistemas utilizados para el mejoramiento del desempeño y seguridad del transporte, no solo de carga sino también de pasajeros y en diferentes modos. El artículo comienza con una descripción sobre los sistemas de transporte ITS, seguido por la presentación de los benefi cios de estos sistemas y fi nalmente se realiza una revisión de las diferentes herramientas que enmarcan los ITS.

  7. Modelling the transport system in China and evaluating the current strategies towards the sustainable transport development

    International Nuclear Information System (INIS)

    Liu, Wen; Lund, Henrik; Mathiesen, Brian Vad

    2013-01-01

    Transport is one of the most challenge sectors when addressing energy security and climate change due to its high reliance on oil products and lack of the alternative fuels. This paper explores the ability of three transport strategies to contribute to the development of a sustainable transport in China. With this purpose in mind, a Chinese transport model has been created and three current transport strategies which are high speed railway (HSR), urban rail transit (URT) and electric vehicle (EV) were evaluated together with a reference transport system in 2020. As conservative results, 13% of the energy saving and 12% of the CO 2 emission reduction can be attained by accomplishing three strategies compared with the reference transport system. However, the energy demand of transport in 2020 with the implementation of three strategies will be about 1.7 times as much as today. The three strategies show the potential of drawing the transport demand to the more energy efficient vehicles; however, more initiatives are needed if the sustainable transport is the long term objective, such as the solutions to stabilise the private vehicle demands, to continuously improve the vehicle efficiency and to boost the alternative fuels produced from the renewable energy sources. - Highlights: • A Chinese transport model was created and three transport strategies were evaluated • Transport is the biggest driver of the oil demand in China not the industry • The energy demand of transport in 2020 will be twice as much as today • Strategies contribute 13% energy saving and 12% CO 2 emission reduction • More initiatives are needed if a sustainable transport is the long-term objective

  8. Data security in Intelligent Transport Systems

    Directory of Open Access Journals (Sweden)

    Tomas Zelinka

    2012-10-01

    Full Text Available Intelligent Transport Services expect availability of the secure seamless communications solutions typically covering widely spread areas. Different ITS solutions require different portfolio of telecommunications service quality. These parameters have to correspond with ITS service performance parameters required by specific service. Even though quite extensive range of public wireless data services with reasonable coverage are provided, most of them are provided with no guaranteed quality and security. ITS requirements can be in most parameters easier reached if multi-path communications systems are applied core solution is combined with both public as well as private ones where and when it is needed. Such solution requires implementation of relevant flexible system architecture supported by the efficient decision processes. This paper is concentrated the telecommunications security issues relevant to the ITS wide area networking. Expected level of security varies in dependence on relevant ITS service requirements. Data volumes transferred both in private data vehicle on board networks as well as between vehicles and infrastructure (C2I or other vehicles (C2C progressively grow. Such trend upsurges the fatal problems appearance probability in case security of the wide area networks is not relevantly treated. That is reason why relevant communications security treatment becomes crucial part of the ITS solution. Besides of available "off shelf" security tools we present solution based on non-public universal identifier with dynamical extension (time and position dependency as an autonomous variables and data selection according to actor role or category. Presented results were obtained within projects e-Ident1, DOTEK2 and SRATVU3.

  9. Geographic information systems - transportation ISTEA management systems server net prototype pooled fund study : phase B - summary

    Science.gov (United States)

    1997-06-01

    The Geographic Information System-Transportation (GIS-T) ISTEA Management Systems Server Net Prototype Pooled Fund Study represents the first national cooperative effort in the transportation industry to address the management and monitoring systems ...

  10. Patterns of Availability and Use of Audiotape Cassettes in Special Libraries. Ph.D. Thesis

    Science.gov (United States)

    Hughes, J. M., II

    1975-01-01

    The availability and use of audiotape cassettes is studied in terms of user requirements. The following factors were examined: how special libraries utilize audiotape cassettes; who the users of the medium are; how the libraries acquire and maintain their collection; and opinions of librarians as to the value of the audiotape cassette as a medium for dissemination of information.

  11. Intelligent transportation system (ITS) international research exchange.

    Science.gov (United States)

    2014-01-01

    ITS applications address surface transportation challenges in safety, mobility, and : sustainability that are similar in cause and impact worldwide. International ITS : exchange allows cooperating nations to benefit from each others pre-competitiv...

  12. Dwight David Eisenhower Transportation Fellowship Program System -

    Data.gov (United States)

    Department of Transportation — DDETFP is a web-based application used to measure the performance of the DDETFP program, enhance reporting, and evaluate the programs' effectiveness in meeting goals...

  13. 2015 International Conference on Information Technology and Intelligent Transportation Systems

    CERN Document Server

    Jain, Lakhmi; Zhao, Xiangmo

    2017-01-01

    This volume includes the proceedings of the 2015 International Conference on Information Technology and Intelligent Transportation Systems (ITITS 2015) which was held in Xi’an on December 12-13, 2015. The conference provided a platform for all professionals and researchers from industry and academia to present and discuss recent advances in the field of Information Technology and Intelligent Transportation Systems. The presented information technologies are connected to intelligent transportation systems including wireless communication, computational technologies, floating car data/floating cellular data, sensing technologies, and video vehicle detection. The articles focusing on intelligent transport systems vary in the technologies applied, from basic management systems to more application systems including topics such as emergency vehicle notification systems, automatic road enforcement, collision avoidance systems and some cooperative systems. The conference hosted 12 invited speakers and over 200 part...

  14. Capacity analysis of an automated kit transportation system

    NARCIS (Netherlands)

    Zijm, W.H.M.; Adan, I.J.B.F.; Buitenhek, R.; Houtum, van G.J.J.A.N.

    2000-01-01

    In this paper, we present a capacity analysis of an automated transportation system in a flexible assembly factory. The transportation system, together with the workstations, is modeled as a network of queues with multiple job classes. Due to its complex nature, the steadystate behavior of this

  15. Urban Transportation Systems in Bogotá and Copenhagen

    DEFF Research Database (Denmark)

    Pineda, Andres Felipe Valderrama; Jørgensen, Ulrik

    2008-01-01

    In this paper we explore the socio-technical dynamics of developing new urban transport systems. Based on the analysis of empirical material from the study of the Transmilenio in Bogotá and the Metro in Copenhagen, we propose that the design, construction and operation of urban transport systems...

  16. Markets and system developments in rail-guided passenger transport

    Energy Technology Data Exchange (ETDEWEB)

    Koch, Guenter [DB International GmbH, Karlsruhe (Germany); Grein, Ottmar [DB International GmbH, Frankfurt am Main (Germany)

    2013-03-01

    The way people are transported is an ever-changing process - and that applies to the railway systems too. If anything, this process of change has sped up in recent years. The authors of this report present an overview of the markets for rail-guided forms of transport and the various systems that exist. (orig.)

  17. Transport Coefficients for dense hard-disk systems

    NARCIS (Netherlands)

    Garcia-Rojo, R.; Luding, Stefan; Brey, J. Javier; Ooms, G.; Hoogendoorn, C.J.

    2007-01-01

    A study of the transport coefficients of a system of elastic hard disks, based on the use of Helfand-Einstein expressions is reported. The pressure, the viscosity, and the heat conductivity are examined for different density and system-size. While most transport coefficients agree with Enskog theory

  18. Advanced transportation system study: Manned launch vehicle concepts for two way transportation system payloads to LEO

    Science.gov (United States)

    Duffy, James B.

    1993-01-01

    The purpose of the Advanced Transportation System Study (ATSS) task area 1 study effort is to examine manned launch vehicle booster concepts and two-way cargo transfer and return vehicle concepts to determine which of the many proposed concepts best meets NASA's needs for two-way transportation to low earth orbit. The study identified specific configurations of the normally unmanned, expendable launch vehicles (such as the National Launch System family) necessary to fly manned payloads. These launch vehicle configurations were then analyzed to determine the integrated booster/spacecraft performance, operations, reliability, and cost characteristics for the payload delivery and return mission. Design impacts to the expendable launch vehicles which would be required to perform the manned payload delivery mission were also identified. These impacts included the implications of applying NASA's man-rating requirements, as well as any mission or payload unique impacts. The booster concepts evaluated included the National Launch System (NLS) family of expendable vehicles and several variations of the NLS reference configurations to deliver larger manned payload concepts (such as the crew logistics vehicle (CLV) proposed by NASA JSC). Advanced, clean sheet concepts such as an F-1A engine derived liquid rocket booster (LRB), the single stage to orbit rocket, and a NASP-derived aerospace plane were also included in the study effort. Existing expendable launch vehicles such as the Titan 4, Ariane 5, Energia, and Proton were also examined. Although several manned payload concepts were considered in the analyses, the reference manned payload was the NASA Langley Research Center's HL-20 version of the personnel launch system (PLS). A scaled up version of the PLS for combined crew/cargo delivery capability, the HL-42 configuration, was also included in the analyses of cargo transfer and return vehicle (CTRV) booster concepts. In addition to strictly manned payloads, two-way cargo

  19. Aspects of transport system management within mining complex using information and telecommunication systems

    Science.gov (United States)

    Semykina, A. S.; Zagorodniy, N. A.; Konev, A. A.; Duganova, E. V.

    2018-05-01

    The paper considers aspects of transport system management within the mining complex. It indicates information and telecommunication systems that are used to increase transportation efficiency. It also describes key advantages and disadvantages. It is found that software products of the Modular Company used in pits allow increasing transport performance, minimizing losses and ensuring efficient transportation of minerals.

  20. The transnational territorial transport system of the Baltic Region

    Directory of Open Access Journals (Sweden)

    Gumenyuk Ivan

    2012-03-01

    Full Text Available In this paper we focus on the structure and territorial borders of the Baltic Sea region, and examine the key structural elements of the transnational territorial transport system. In this respect, we clarify some terms used in transport geography. For the first time the transport system gets territorially localized, which allows for a broad range of new studies of transnational transportation in the Baltic Sea area. We also identify the main principles of development and operation of international territorial transport systems and present them taking the Baltic Sea region as an example. Our findings, we hope, will have a great practical application for researchers of transport geography, especially those studying international logistics.

  1. Internal transport barrier physics in helical systems

    International Nuclear Information System (INIS)

    Yokoyama, M.; Minami, T.; Fujisawa, A.; Herranz, J.; Ida, K.; Yamagishi, O.; Yamada, H.; Maaberg, H.; Beidler, C.D.; Dinklage, A.; Estrada, T.; Castejon, F.; Murakami, S.

    2005-01-01

    The electron internal transport barrier (eITB) has been observed in wide range of helical systems, such as CHS [eg.,1], LHD [eg., 2], TJ-II [eg., 3] and W7-AS [eg., 4]. The eITB isA defined as highly peaked electron temperature (Te) profile with strongly positive radial electric field (Er) in the central region. These observations are reviewed in this paper to understand the device-independent common findings and also to draw the main differences. This is the first report from the International Stellarator Profile Database Activity. The formation of the strong central positive Er has been understood mainly as a result of the ambipolarity of neoclassical electron and ion fluxes, although some additional convective electron flux such as driven by ECRH is required in some situations. This 'neoclassical' physics peculiar to low collisional regime of helical plasmas provides the commonly observed existence of the ECRH power threshold (which is also depending on the density). This is contrastive characteristics to the ITB observed in tokamaks. The dependence of the ECRH power threshold on the magnetic configuration and on the heating scenario among these devices are currently being examined by taking the effective ripple and the trapped particle fraction as parameters to achieve the comprehensive understanding. The roles of low order rational surfaces on the onset of eITB formation and also on its radial size (location of the footpoint of the eITB) have been indicated in inward shifted configurations in LHD (depending on the relative locations of heating position and 2/1 island) and TJ-II (eITB becomes possible at higher density when 3/2 rational is introduced in the plasma core region). It is speculated that, for the latter case, the resonance causes an extra electron flux to trigger the positive Er. The interplay between low order rational surfaces and the formation of eITB still waits for the systematic experiment and theoretical analysis. The external controllability

  2. Improvement of urban passenger transport ticketing systems by deploying intelligent transport systems

    Directory of Open Access Journals (Sweden)

    G. Jakubauskas

    2006-12-01

    Full Text Available The main advantages and disadvantages of conventional and intelligent ticketing systems and possible positive outcomes when introducing intelligent transport solutions – namely smart cards or e‑ticketing instead of conventional ones (paper tickets and magnetic cards are analysed in the paper. Two ideas of creating an intelligent ticketing system in an urban public transport are scrutinized. The first is electronic ticket and related equipment, the second – e-ticket and functional areas of it. In the article analysis has also been made on practical outcomes related with introduction of smart cards and e-ticketing. Practical tests and trials as well as a subsequent implementation of electronic tickets have proved unchallenged advantages of contactless smart cards against the contact ones. Nevertheless, a new age of modern technologies calls even for more effective solutions – namely virtual-ticketing systems that might be achieved through introduction of mobile technologies. Therefore, the main focus in the paper is made on the analysis of e-ticket.

  3. Decarbonizing Sweden’s energy and transportation system by 2050

    Directory of Open Access Journals (Sweden)

    Rasmus Bramstoft

    2017-01-01

    Full Text Available Decarbonizing Sweden’s transportation sector is necessary to realize its long-term vision of eliminating net greenhouse gas (GHG emissions from the energy system by 2050. Within this context, this study develops two scenarios for the transportation sector: one with high electrification (EVS and the other with high biofuel and biomethane utilization (BIOS. The energy system model STREAM is utilized to compute the socioeconomic system cost and simulate an integrated transportation, electricity, gas, fuel refinery, and heat system. The results show that electrifying a high share of Sweden’s road transportation yields the least systems cost. However, in the least-cost scenario (EVS, bioenergy resources account for 57% of the final energy use in the transportation sector. Further, a sensitivity analysis shows that the costs of different types of cars are the most sensitive parameters in the comparative analysis of the scenarios.

  4. Decarbonizing Sweden’s energy and transportation system by 2050

    DEFF Research Database (Denmark)

    Bramstoft, Rasmus; Skytte, Klaus

    2017-01-01

    Decarbonizing Sweden’s transportation sector is necessary to realize its long-term vision of eliminating net greenhouse gas (GHG) emissions from the energy system by 2050. Within this context, this study develops two scenarios for the transportation sector: one with high electrification (EVS......) and the other with high biofuel and biomethane utilization (BIOS). The energy system model STREAM is utilized to compute the socioeconomic system cost and simulate an integrated transportation, electricity, gas, fuel refinery, and heat system. The results show that electrifying a high share of Sweden’s road...... transportation yields the least systems cost. However, in the least-cost scenario (EVS), bioenergy resources account for 57% of the final energy use in the transportation sector. Further, a sensitivity analysis shows that the costs of different types of cars are the most sensitive parameters in the comparative...

  5. Public transport traffic management systems simulation in Craiova city

    Energy Technology Data Exchange (ETDEWEB)

    Racila, L.

    2016-07-01

    Urban transport is a comprehensive and dynamic mechanism. Therefore, all the problems for improving and reorganization of the system can be examined only in the light of a systemic approach. Currently, public passenger transport is one of the most important branches of the urban development in cities and metropolis. Public passenger transport activity and all the steps taken to improve that activity are considered to be of great social importance. In the current stage of city development, one of the main tasks is to create a public passenger transportation system that is safe, affordable, economical, reliable and environmentally friendly. The important role of passenger transport in the city's economy and achieving important social services to the population, dictates the need to introduce measures in the system that are harmonious, balanced and effective. This can only be done, in the context of current development, only after the system as a whole is tested extensively through special traffic and management software. (Author)

  6. Critical review of expert system validation in transportation

    Science.gov (United States)

    1997-01-01

    Expert system validationthat is, testing systems to ascertain whether they achieve acceptable performance levelshas with few exceptions been ad hoc, informal, and of dubious value. Very few efforts have been made in this regard in the transport...

  7. Environmental management system for transportation maintenance operations : [technical brief].

    Science.gov (United States)

    2014-04-01

    This report provides the framework for the environmental management system to analyze : greenhouse gas emissions from transportation maintenance operations. The system enables user : to compare different scenarios and make informed decisions to minim...

  8. FY17 Transportation and Hydrogen Systems Center Journal Publication Highlights

    Energy Technology Data Exchange (ETDEWEB)

    2017-12-08

    NREL's Transportation and Hydrogen Systems Center published 39 journal articles in fiscal year 2017 highlighting recent research in advanced vehicle technology, alternative fuels, and hydrogen systems.

  9. Decentralized control of multi-agent aerial transportation system

    KAUST Repository

    Toumi, Noureddine

    2017-01-01

    and Landing aircraft (VTOL) transportation system. We develop a decentralized method. The advantage of such a solution is that it can provide better maneuverability and lifting capabilities compared to existing systems. First, we consider a cooperative group

  10. Road Transport Management System (RTMS): a self regulation initiative in heavy vehicle transport in South Africa

    CSIR Research Space (South Africa)

    Nordengen, Paul A

    2007-07-01

    Full Text Available This paper describes the most recent developments of an initiative to introduce meaningful self-regulation in the heavy vehicle transport industry through a Road Transport Management System (RTMS) with the aim of contributing to the road authorities...

  11. Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.

    Science.gov (United States)

    Ast, Cindy; Foret, Jessica; Oltrogge, Luke M; De Michele, Roberto; Kleist, Thomas J; Ho, Cheng-Hsun; Frommer, Wolf B

    2017-09-05

    Sensitivity, dynamic and detection range as well as exclusion of expression and instrumental artifacts are critical for the quantitation of data obtained with fluorescent protein (FP)-based biosensors in vivo. Current biosensors designs are, in general, unable to simultaneously meet all these criteria. Here, we describe a generalizable platform to create dual-FP biosensors with large dynamic ranges by employing a single FP-cassette, named GO-(Green-Orange) Matryoshka. The cassette nests a stable reference FP (large Stokes shift LSSmOrange) within a reporter FP (circularly permuted green FP). GO- Matryoshka yields green and orange fluorescence upon blue excitation. As proof of concept, we converted existing, single-emission biosensors into a series of ratiometric calcium sensors (MatryoshCaMP6s) and ammonium transport activity sensors (AmTryoshka1;3). We additionally identified the internal acid-base equilibrium as a key determinant of the GCaMP dynamic range. Matryoshka technology promises flexibility in the design of a wide spectrum of ratiometric biosensors and expanded in vivo applications.Single fluorescent protein biosensors are susceptible to expression and instrumental artifacts. Here Ast et al. describe a dual fluorescent protein design whereby a reference fluorescent protein is nested within a reporter fluorescent protein to control for such artifacts while preserving sensitivity and dynamic range.

  12. Efficiency increase of complex production and transport systems management

    Directory of Open Access Journals (Sweden)

    Kornilov S.

    2017-01-01

    Full Text Available This article deals with the problem of the reduced efficiency of management in complex production - transport systems due to the lack of co-ordination in the operation of industrial enterprises and transport carrying out their maintenance. The existing transport service schedules for auxiliary departments do not take into account possible changes in operating conditions, the probability of malfunctions and the amount of reserves, which leads to an increase in general production costs. To solve this problem, we propose to use the interval regulation of production and transport processes in all departments of the complex production and transport systems. Also, such regulation involves the determination of traffic service priority. This will allow passing on from the regulated control of production and transport processes to the situational one, adapted to specific conditions, and reducing losses from untimely transport servicing, which will lead to a stores reduction and efficiency increase of the enterprise circulating facilities use. Testing the effectiveness of interval regulation was performed on the system and dynamics simulation model of liquid iron transportation in the oxygen converter shop of the metallurgical enterprise. It was established that the use of interval regulation processes in iron production and its transportation will allow decreasing non-productive downtime by 21% and the amount of the liquid iron in anticipation of recasting in the oxygen converter shop – by 33%. Economical effect of reducing the liquid iron downtime during transportation to the oxygen converter shop will be about 30 million rubles per year.

  13. Radioactive waste transportation systems analysis and program plan

    International Nuclear Information System (INIS)

    Shappert, L.B.; Joy, D.S.; Heiskell, M.M.

    1978-03-01

    The objective of the Transportation/Logistics Study is to ensure the availability of a viable system for transporting the wastes to a federal repository in 1985. In order to accomplish this objective, a systems analysis of waste transportation has been directed by ORNL to determine the problems that must be solved and to develop a program plan that identifies which problems must first be pursued. To facilitate this overall approach and to provide for short- and long-range waste management, logistics models have been developed to determine the transportation fleet requirements and costs. Results of the study are described in this report

  14. Third-Order Transport with MAD Input: A Computer Program for Designing Charged Particle Beam Transport Systems

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Karl

    1998-10-28

    TRANSPORT has been in existence in various evolutionary versions since 1963. The present version of TRANSPORT is a first-, second-, and third-order matrix multiplication computer program intended for the design of static-magnetic beam transport systems.

  15. Environmental assessment of 9 European public bus transportation systems

    DEFF Research Database (Denmark)

    Nanaki, Eva; Koroneos, Christophis; Roset, Jaume

    2017-01-01

    that affect the production of carbon dioxide (CO2) as well as of air pollutants, in 9 major European cities, aiming to provide a comprehensive overview of the actual knowledge on the atmospheric pollution from public transportation systems. CO2 emissions as well as air pollutants, such as CO, HC, PM, NOx......The transportation sector is one of the largest sources of EU’s greenhouse gas emissions. In 2011, transportation represented approximately 25 percent of total EU’s greenhouse gas emissions. Urban mobility accounts for 40% of all CO2 emissions of road transport and up to 70% of other pollutants...... from transport. As, transportation and mobility play a crucial part both in urban economics and the quality of life, it is of great significance to ensure a low carbon transportation sector, so as to deal with the threat that climate change poses to urban areas. This study examines the factors...

  16. A system for transportation of hydrocarbons at sea; System for transport av hydrokarboner til havs

    Energy Technology Data Exchange (ETDEWEB)

    Breivik, K.; Smedal, A.

    1997-04-01

    To exploit the offshore oil and gas fields economically it is important to use suitable methods and equipment for transporting the oil and gas to the landing sites. The present invention concerns a system for transportation of hydrocarbons to the landing sites. It consists of one or more loading buoys and a number of preferably conventional tankers and at least one offshore loading ship arranged to receive hydrocarbons from the loading buoy to which it is connected by means of a coupling device in the ship`s bow. The tankers have in their sterns arrangements to convey the fluid through flexible tubes from complementary arrangements in the bow of the loading ship. The loading ship and the tanker have a position reference system for mutual dynamical positioning. The entire operation of connecting the tubes and transferring the fluid flow takes place with both ships moving, at least at steerage way. The invention aims to provide efficient use of the expensive special ships that are needed to communicate with the loading buoys. Another aim is to reduce the need for expensive specialized equipment onboard both the loading ships and the tankers. 5 figs.

  17. Shenzhen Comprehensive Transport System Planning:An Exploration of Sustainable Urban Transport Development on Condition of Limited Resources

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    With "integration" as the direction,Shenzhen Comprehensive Transport Planning integrates the plan,construction and management of all kinds of transport mode in the transport system,and integrates the transport with the social,economic and environment development.The planning specifies the strategic targets,key indicators,development strategies as well as major policies of the comprehensive transport system,which explores an alternative way for the sustainable urban transport development under the condition of limited resources in Shenzhen.

  18. United States Department of Energy Automated Transportation Management System

    International Nuclear Information System (INIS)

    Portsmouth, J.H.

    1992-01-01

    At the US Department of Energy (DOE) 80 transportation facilities, each contractor's transportation management operation has different internal and site specific procedures, and reports to a DOE regional Field Office Traffic Manager (FOTM). The DOE Transportation Management Program (TMP) has the responsibility to manage a transportation program for safe, efficient, and economical transportation of DOE-owned materials. The TMP develops and administers transportation/traffic operations management policies and programs for materials; including radioactive materials, other hazardous materials, hazardous substances, and hazardous wastes, pursuant to applicable federal regulations, such as the Code of Federal Register, Sections 40 and 49. Transportation management has become an increasingly critical primarily because of transportation issues regarding the shipment of radioactive materials and hazardous wastes that are frequently the focus of public concerns. A large shipments and requiring millions of business transactions necessitates the establishment of automated systems, programs, procedures, and controls to ensure that the transportation management process in being handled in a safe, efficient, and economical manner. As the mission of many DOE facilities changes from production of special nuclear materials for defense purposes to environmental restoration and waste management, the role of transportation management will become even more important to the safe and efficient movement of waste materials to prescribed locations. In support of this role, the Automated Transportation Management System (ATMS) was conceived to assist the DOE and its contractors in the performance of their day-to-day transportation management activities. The ATMS utilizes the latest in technology and will supply state-of-the-art automated transportation management for current and future DOE transportation requirements

  19. Study on tracking system for radioactive material transport

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, F.; Igarashi, M.; Nomura, T. [Nuclear Emergency Assistance and Training Center, Japan Nuclear Cycle Development Inst., Ibaraki (Japan); Nakagome, Y. [Research Reactor Inst., Kyoto Univ., Osaka (Japan)

    2004-07-01

    When a transportation accident occurs, all entities including the shipper, the transportation organization, local governments, and emergency response organizations must have organized and planned for civil safety, property, and environmental protection. When a transportation accident occurs, many related organizations will be involved, and their cooperation determines the success or failure of the response. The point where the accident happens cannot be pinpointed in advance. Nuclear fuel transportation also requires a quick response from a viewpoint of security. A tracking system for radioactive material transport is being developed for use in Japan. The objective of this system is, in the rare event of an accident, for communication capabilities to share specific information among relevant organizations, the transporter, and so on.

  20. Innovating for a competitive and resource-efficient transport system

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-05-15

    Transport is vital to the economic prosperity and social integration of Europe. EU-transport policy is directed to developing a smart, efficient transport system with reduced dependency on fossil fuels and less environmental impacts that will enhance mobility in Europe and will underpin Europe's competitiveness in global markets. This includes the transport sector itself, which is an important part of the EU economy. In contributing to achieving these ambitious goals, extensive investments are made in research and development for sustainable and innovative solutions. This Policy Brochure, which is produced by the Transport Research and Innovation Portal (TRIP), highlights the contribution of research, development, and innovation in securing a competitive and resource-efficient transport system in Europe.

  1. Study on tracking system for radioactive material transport

    International Nuclear Information System (INIS)

    Watanabe, F.; Igarashi, M.; Nomura, T.; Nakagome, Y.

    2004-01-01

    When a transportation accident occurs, all entities including the shipper, the transportation organization, local governments, and emergency response organizations must have organized and planned for civil safety, property, and environmental protection. When a transportation accident occurs, many related organizations will be involved, and their cooperation determines the success or failure of the response. The point where the accident happens cannot be pinpointed in advance. Nuclear fuel transportation also requires a quick response from a viewpoint of security. A tracking system for radioactive material transport is being developed for use in Japan. The objective of this system is, in the rare event of an accident, for communication capabilities to share specific information among relevant organizations, the transporter, and so on

  2. Characterization of SiaA, a streptococcal heme-binding protein associated with a heme ABC transport system.

    Science.gov (United States)

    Sook, Brian R; Block, Darci R; Sumithran, Suganya; Montañez, Griselle E; Rodgers, Kenton R; Dawson, John H; Eichenbaum, Zehava; Dixon, Dabney W

    2008-02-26

    Many pathogenic bacteria require heme and obtain it from their environment. Heme transverses the cytoplasmic membrane via an ATP binding cassette (ABC) pathway. Although a number of heme ABC transport systems have been described in pathogenic bacteria, there is as yet little biophysical characterization of the proteins in these systems. The sia (hts) gene cluster encodes a heme ABC transporter in the Gram positive Streptococcus pyogenes. The lipoprotein-anchored heme binding protein (HBP) of this transporter is SiaA (HtsA). In the current study, resonance Raman (rR), magnetic circular dichroism (MCD), and nuclear magnetic resonance (NMR) spectroscopies were used to determine the coordination state and spin state of both the ferric and ferrous forms of this protein. Identifiers from these techniques suggest that the heme is six-coordinate and low-spin in both oxidation states of the protein, with methionine and histidine as axial ligands. SiaA has a pKa of 9.7 +/- 0.1, attributed to deprotonation of the axial histidine. Guanidinium titration studies show that the ferric state is less stable than the ferrous state, with DeltaG(H2O) values for the oxidized and reduced proteins of 7.3 +/- 0.8 and 16.0 +/- 3.6 kcal mol-1, respectively. The reductive and oxidative midpoint potentials determined via spectroelectrochemistry are 83 +/- 3 and 64 +/- 3 mV, respectively; the irreversibility of heme reduction suggests that redox cycling of the heme is coupled to a kinetically sluggish change in structure or conformation. The biophysical characterization described herein will significantly advance our understanding of structure-function relationships in HBP.

  3. Salt Repository Project transportation system interface requirements: Transportation system/repository receiving facility interface requirements

    International Nuclear Information System (INIS)

    Smith, L.A.; Insalaco, J.W.; Trainer, T.A.

    1988-01-01

    This report is a preliminary review of the interface between the transportation system and the repository receiving facility for a nuclear waste mined geologic disposal system in salt. Criteria for generic cask and facility designs are developed. These criteria are derived by examining the interfaces that occur as a result of the operations needed to receive nuclear waste at a repository. These criteria provide the basis for design of a safe, operable, practical nuclear waste receiving facility. The processing functions required to move the shipping unit from the gate into the unloading area and back to the gate for dispatch are described. Criteria for a generic receiving facility are discussed but no specific facility design is presented or evaluated. The criteria are stated in general terms to allow application to a wide variety of cask and facility designs. 9 refs., 6 figs., 4 tabs

  4. Control system of fuel transporting device

    International Nuclear Information System (INIS)

    Yokota, Minoru.

    1981-01-01

    Purpose: To effectively avoid an obstacle in a fuel transporting device by reading the outputs of absolute position detectors mounted on movable trucks, controlling the movements of the trucks, and thereby smoothly and accurately positioning the fuel transporting device at predetermined position and providing a contact detector thereat. Method: The outputs from absolute position detectors which are mounted on a longitudinally movable truck and a laterally movable truck are input to an input/output control circuit. The input/output control circuit serves to compare, the position a fuel transporting device is to be moved to, with the present position on the basis of said input detection signal and a command signal from an operator console, to calculate the amount of movement to be driven, to produce an operation signal therefor to a control panel, and to drive and control the drive motors which are respectively mounted on the trucks for the fuel transfer device. On the other hand, in case that the transfer device comes into contact with an obstacle, the contact detector will immediately operate to produce a stop command through the control panel to the transporting device, and avoid a collision with the obstacle. (Yoshino, Y.)

  5. Stochasticity and transport in Hamiltonian systems

    International Nuclear Information System (INIS)

    MacKay, R.S.; Meiss, J.D.; Percival, I.C.

    1983-08-01

    The theory of transport in nonlinear dynamics is developed in terms of leaky barriers which remain when invariant tori are destroyed. We describe the organization of stochastic motion by these barriers and give an explanation of long-time correlations in the stochastic regime

  6. Inelastic transport theory for nanoscale systems

    DEFF Research Database (Denmark)

    Frederiksen, Thomas

    2007-01-01

    This thesis describes theoretical and numerical investigations of inelastic scat- tering and energy dissipation in electron transport through nanoscale sys- tems. A computational scheme, based on a combination of density functional theory (DFT) and nonequilibrium Green’s functions (NEGF), has been...

  7. Control of machine functions or transport systems

    International Nuclear Information System (INIS)

    Woodley, M.D.; Lee, M.J.; Jaeger, J.; King, A.S.

    1983-01-01

    A computer code, COMFORT, has been developed at SLAC for on-line calculation of the strengths of magnetic elements in an electron storage ring or transport beam line, subject to first order fitting constraints on the ring or beam line parameters. This code can also be used off-line as an interactive lattice or beam line design tool

  8. Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria

    Energy Technology Data Exchange (ETDEWEB)

    Herrou, Julien; Willett, Jonathan W.; Czy; #380; , Daniel M.; Babnigg, Gyorgy; Kim, Youngchang; Crosson, Sean (UC)

    2016-12-19

    ABSTRACT

    Brucella abortusσE1is an EcfG family sigma factor that regulates the transcription of dozens of genes in response to diverse stress conditions and is required for maintenance of chronic infection in a mouse model. A putative ATP-binding cassette transporter operon,bab1_0223-bab1_0226, is among the most highly activated gene sets in the σE1regulon. The proteins encoded by the operon resemble quaternary ammonium-compatible solute importers but are most similar in sequence to the broadly conserved YehZYXW system, which remains largely uncharacterized. Transcription ofyehZYXWis activated by the general stress sigma factor σSinEnterobacteriaceae, which suggests a functional role for this transport system in bacterial stress response across the classesAlphaproteobacteriaandGammaproteobacteria. We present evidence thatB. abortusYehZYXW does not function as an importer of known compatible solutes under physiological conditions and does not contribute to the virulence defect of a σE1-null strain. The solein vitrophenotype associated with genetic disruption of this putative transport system is reduced growth in the presence of high Li+ion concentrations. A crystal structure ofB. abortusYehZ revealed a class II periplasmic binding protein fold with significant structural homology toArchaeoglobus fulgidusProX, which binds glycine betaine. However, the structure

  9. Solute carrier transporters: potential targets for digestive system neoplasms.

    Science.gov (United States)

    Xie, Jing; Zhu, Xiao Yan; Liu, Lu Ming; Meng, Zhi Qiang

    2018-01-01

    Digestive system neoplasms are the leading causes of cancer-related death all over the world. Solute carrier (SLC) superfamily is composed of a series of transporters that are ubiquitously expressed in organs and tissues of digestive systems and mediate specific uptake of small molecule substrates in facilitative manner. Given the important role of SLC proteins in maintaining normal functions of digestive system, dysregulation of these protein in digestive system neoplasms may deliver biological and clinical significance that deserves systemic studies. In this review, we critically summarized the recent advances in understanding the role of SLC proteins in digestive system neoplasms. We highlighted that several SLC subfamilies, including metal ion transporters, transporters of glucose and other sugars, transporters of urea, neurotransmitters and biogenic amines, ammonium and choline, inorganic cation/anion transporters, transporters of nucleotide, amino acid and oligopeptide organic anion transporters, transporters of vitamins and cofactors and mitochondrial carrier, may play important roles in mediating the initiation, progression, metastasis, and chemoresistance of digestive system neoplasms. Proteins in these SLC subfamilies may also have diagnostic and prognostic values to particular cancer types. Differential expression of SLC proteins in tumors of digestive system was analyzed by extracting data from human cancer database, which revealed that the roles of SLC proteins may either be dependent on the substrates they transport or be tissue specific. In addition, small molecule modulators that pharmacologically regulate the functions of SLC proteins were discussed for their possible application in the treatment of digestive system neoplasms. This review highlighted the potential of SLC family proteins as drug target for the treatment of digestive system neoplasms.

  10. Transport and Power System Scenarios for Northern Europe in 2030

    DEFF Research Database (Denmark)

    Juul, Nina; Meibom, Peter

    2009-01-01

    it is assumed that power can go both from grid-to-vehicle and vehicle-to-grid. Oil prices are assumed to be $120/barrel, and CO2 price 40 €/ton. This results in an optimal investment path with a large increase in sustainable energy; primarily wind energy, as well as an increase in the electric drive vehicles......Increasing focus on sustainability affects all parts of the energy system. Integrating the power and transport system in future energy system planning, influences the economically optimal investments and optimal operation of the power system as well as the transport system. This work presents...... analysis of the optimal configuration and operation of the integrated power and transport system in Northern Europe. Optimal configuration and operation is obtained using the optimisation model, Balmorel [1], with a transport model extension. For electric drive vehicles with plug-in capabilities...

  11. Reaction-Transport Systems Mesoscopic Foundations, Fronts, and Spatial Instabilities

    CERN Document Server

    Horsthemke, Werner; Mendez, Vicenc

    2010-01-01

    This book is an introduction to the dynamics of reaction-diffusion systems, with a focus on fronts and stationary spatial patterns. Emphasis is on systems that are non-standard in the sense that either the transport is not simply classical diffusion (Brownian motion) or the system is not homogeneous. A important feature is the derivation of the basic phenomenological equations from the mesoscopic system properties. Topics addressed include transport with inertia, described by persistent random walks and hyperbolic reaction-transport equations and transport by anomalous diffusion, in particular subdiffusion, where the mean square displacement grows sublinearly with time. In particular reaction-diffusion systems are studied where the medium is in turn either spatially inhomogeneous, compositionally heterogeneous or spatially discrete. Applications span a vast range of interdisciplinary fields and the systems considered can be as different as human or animal groups migrating under external influences, population...

  12. Polycistronic transcription of fused cassettes and identification of translation initiation signals in an unusual gene cassette array from Pseudomonas aeruginosa [version 3; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Érica L. Fonseca

    2015-11-01

    Full Text Available The gene cassettes found in class 1 integrons are generally promoterless units composed by an open reading frame (ORF, a short 5’ untranslated region (UTR and a 3’ recombination site (attC. Fused gene cassettes are generated by partial or total loss of the attC from the first cassette in an array, creating, in some cases, a fusion with the ORF from the next cassette. These structures are rare and little is known about their mechanisms of mobilization and expression. The aim of this study was to evaluate the dynamic of mobilization and transcription of the gcu14-blaGES-1/aacA4 gene cassette array, which harbours a fused gene cassette represented by blaGES-1/aacA4. The cassette array was analyzed by Northern blot and real-time reverse transcription-polymerase chain reaction (RT-PCR in order to assess the transcription mechanism of blaGES-1/aacA4 fused cassette. Also, inverse polymerase chain reactions (PCR were performed to detect the free circular forms of gcu14, blaGES-1 and aacA4. The Northern blot and real time RT-PCR revealed a polycistronic transcription, in which the fused cassette blaGES-1/aacA4 is transcribed as a unique gene, while gcu14 (with a canonical attC recombination site has a monocistronic transcription. The gcu14 cassette, closer to the weak configuration of cassette promoter (PcW, had a higher transcription level than blaGES-1/aacA4, indicating that the cassette position affects the transcript amounts. The presence of ORF-11 at attI1, immediately preceding gcu14, and of a Shine-Dalgarno sequence upstream blaGES-1/aacA4 composes a scenario for the occurrence of array translation. Inverse PCR generated amplicons corresponding to gcu14, gcu14-aacA4 and gcu14-blaGES-1/aacA4 free circular forms, but not to blaGES-1 and aacA4 alone, indicating that the GES-1 truncated attC is not substrate of integrase activity and that these genes are mobilized together as a unique cassette. This study was original in showing the transcription

  13. Human behavior research and the design of sustainable transport systems

    Science.gov (United States)

    Schauer, James J.

    2011-09-01

    Transport currently represents approximately 19% of the global energy demand and accounts for about 23% of the global carbon dioxide emissions (IEA 2009). As the demand for mobility is expected to continue to increase in the coming decades, the stabilization of atmospheric carbon dioxide levels will require the evolution of transport, along with power generation, building design and manufacturing. The continued development of these sectors will need to include changes in energy sources, energy delivery, materials, infrastructure and human behavior. Pathways to reducing carbon from the transport sector have unique challenges and opportunities that are inherent to the human choices and behavioral patterns that mold the transportation systems and the associated energy needs. Technology, government investment, and regulatory policies have a significant impact on the formulation of transportation infrastructure; however, the role of human behavior and public acceptance on the efficiency and effectiveness of transport systems should not be underestimated. Although developed, rapidly developing, and underdeveloped nations face different challenges in the establishment of transport infrastructure that can meet transport needs while achieving sustainable carbon dioxide emissions, the constraints that establish the domain of possibilities are closely related for all nations. These constraints include capital investment, fuel supplies, power systems, and human behavior. Throughout the world, there are considerable efforts directed at advancing and optimizing the financing of sustainable infrastructures, the production of low carbon fuels, and the production of advanced power systems, but the foundational work on methods to understand human preferences and behavior within the context of transport and the valuation of reductions in carbon dioxide emissions is greatly lagging behind. These methods and the associated understanding of human behavior and the willingness to pay for

  14. Electrical Power Systems for NASA's Space Transportation Program

    Science.gov (United States)

    Lollar, Louis F.; Maus, Louis C.

    1998-01-01

    Marshall Space Flight Center (MSFC) is the National Aeronautics and Space Administration's (NASA) lead center for space transportation systems development. These systems include earth to orbit launch vehicles, as well as vehicles for orbital transfer and deep space missions. The tasks for these systems include research, technology maturation, design, development, and integration of space transportation and propulsion systems. One of the key elements in any transportation system is the electrical power system (EPS). Every transportation system has to have some form of electrical power and the EPS for each of these systems tends to be as varied and unique as the missions they are supporting. The Preliminary Design Office (PD) at MSFC is tasked to perform feasibility analyses and preliminary design studies for new projects, particularly in the space transportation systems area. All major subsystems, including electrical power, are included in each of these studies. Three example systems being evaluated in PD at this time are the Liquid Fly Back Booster (LFBB) system, the Human Mission to Mars (HMM) study, and a tether based flight experiment called the Propulsive Small Expendable Deployer System (ProSEDS). These three systems are in various stages of definition in the study phase.

  15. Pouring and running a protein gel by reusing commercial cassettes.

    Science.gov (United States)

    Hwang, Alexander C; Grey, Paris H; Cuddy, Katrina; Oppenheimer, David G

    2012-02-12

    The evaluation of proteins using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis is a common technique used by biochemistry and molecular biology researchers. For laboratories that perform daily analyses of proteins, the cost of commercially available polyacrylamide gels (~$10/gel) can be considerable over time. To mitigate this cost, some researchers prepare their own polyacrylamide gels. Traditional methods of pouring these gels typically utilize specialized equipment and glass gel plates that can be expensive and preclude pouring many gels and storing them for future use. Furthermore, handling of glass plates during cleaning or gel pouring can result in accidental breakage creating a safety hazard, which may preclude their use in undergraduate laboratory classes. Our protocol demonstrates how to pour multiple protein gels simultaneously by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment. In addition, plastic gel cassettes are extremely resistant to breakage, which makes them ideal for undergraduate laboratory classrooms.

  16. Space transportation systems within ESA programmes: Current status and perspectives

    Science.gov (United States)

    Delahais, Maurice

    1993-03-01

    An overview of the space transportation aspects of the ESA (European Space Agency) programs as they result from history, present status, and decisions taken at the ministerial level conference in Granada, Spain is presented. The new factors taken into consideration for the long term plan proposed in Munich, Germany, the three strategic options for the reorientation of the ESA long term plan, and the essential elements of space transportation in the Granada long term plan in three areas of space activities, scientific, and commercial launches with expendable launch vehicles, manned flight and in-orbit infrastructure, and future transportation systems are outlined. The new ESA long term plan, in the field of space transportation systems, constitutes a reorientation of the initial program contemplated in previous councils at ministerial level. It aims at balancing the new economic situation with the new avenues of cooperation, and the outcome will be a new implementation of the space transportation systems policy.

  17. Natural hazard impacts on transport systems: analyzing the data base of transport accidents in Russia

    Science.gov (United States)

    Petrova, Elena

    2015-04-01

    We consider a transport accident as any accident that occurs during transportation of people and goods. It comprises of accidents involving air, road, rail, water, and pipeline transport. With over 1.2 million people killed each year, road accidents are one of the world's leading causes of death; another 20-50 million people are injured each year on the world's roads while walking, cycling, or driving. Transport accidents of other types including air, rail, and water transport accidents are not as numerous as road crashes, but the relative risk of each accident is much higher because of the higher number of people killed and injured per accident. Pipeline ruptures cause large damages to the environment. That is why safety and security are of primary concern for any transport system. The transport system of the Russian Federation (RF) is one of the most extensive in the world. It includes 1,283,000 km of public roads, more than 600,000 km of airlines, more than 200,000 km of gas, oil, and product pipelines, 115,000 km of inland waterways, and 87,000 km of railways. The transport system, especially the transport infrastructure of the country is exposed to impacts of various natural hazards and weather extremes such as heavy rains, snowfalls, snowdrifts, floods, earthquakes, volcanic eruptions, landslides, snow avalanches, debris flows, rock falls, fog or icing roads, and other natural factors that additionally trigger many accidents. In June 2014, the Ministry of Transport of the RF has compiled a new version of the Transport Strategy of the RF up to 2030. Among of the key pillars of the Strategy are to increase the safety of the transport system and to reduce negative environmental impacts. Using the data base of technological accidents that was created by the author, the study investigates temporal variations and regional differences of the transport accidents' risk within the Russian federal regions and a contribution of natural factors to occurrences of different

  18. One-Loop Operation of Primary Heat Transport System in MONJU During Heat Transport System Modifications

    International Nuclear Information System (INIS)

    Goto, T.; Tsushima, H.; Sakurai, N.; Jo, T.

    2006-01-01

    MONJU is a prototype fast breeder reactor (FBR). Modification work commenced in March 2005. Since June 2004, MONJU has changed to one-loop operation of the primary heat transport system (PHTS) with all of the secondary heat transport systems (SHTS) drained of sodium. The purposes of this change are to shorten the modification period and to reduce the cost incurred for circuit trace heating electrical consumption. Before changing condition, the following issues were investigated to show that this mode of operation was possible. The heat loss from the reactor vessel and the single primary loop must exceed the decay heat by an acceptable margin but the capacity of pre-heaters to keep the sodium within the primary vessel at about 200 deg. C must be maintained. With regard to the heat loss and the decay heat, the estimated heat loss in the primary system was in the range of 90-170 kW in one-loop operation, and the calculated decay heat was 21.2 kW. Although the heat input of the primary pump was considered, it was clear that circuit heat loss greatly exceeded the decay heat. As for pre-heaters, effective capacity was less than the heat loss. Therefore, the temperature of the reactor vessel room was raised to reduce the heat loss. One-loop operation of the PHTS was able to be executed by means of these measures. The cost of electrical consumption in the power plant has been reduced by one-loop operation of the PHTS and the modification period was shortened. (authors)

  19. Development of hotcell transportation system technology for high radioactive material

    International Nuclear Information System (INIS)

    Seo, K. S.; Seo, C. S.; Lee, J. C.

    2012-04-01

    In the first stage of the research, the transportation and storage characteristics analysis of the pyroprocess materials, the development of horizontal type hot cell transportation system, and the design of interim storage system for the pyroprocess material are conducted. The optimized capacity, transportation frequency and operation period of pyroprocess facility are found using the logistics analysis program developed in this project. A new hot cell transportation system was designed. Through the safety analysis and test for the hot cell transportation system, the design license has been approved. A new type hot cell docking system with superior performance has been developed with a patented rotating lid system. We have reached to a unique concept of interim storage of pyroprocess materials and selected a system through a comparative evaluation of existing ones. In the second stage of the research, transportation/storage/sealing devices for PRIDE recovered material/wastes were developed. And test model for the devices in engineering scale facility were also developed. The design requirements for a vertical docking system were evaluated and the performance assessment using a scaled mock-up was conducted. Integrated storage management technology was evaluated for an efficient management of process materials. A heat transfer simulation and characteristics analysis for the storage system were conducted. The derivation of design requirements, design and fabrication of a canister test model, and preliminary safety assessment were conducted

  20. Sintering boat transport system for the SAF line

    International Nuclear Information System (INIS)

    Egli, W.; Bogart, R.L.

    1983-10-01

    The Secure Automated Fabrication (SAF) line will be a remotely operated process for the manufacture of breeder reactor fuel pins. The sintering boat transport system will service the pellet operations. Since the Boat Transport System will be the major link between several subsystem operations, reliability and ease of maintenance are prime requirements for the design. A prototypic version of the Boat Transport System was designed, built and tested to verify the operability of the selected approach. Extensive testing provided valuable input to the final design and substantiated the soundness of the concept

  1. Transportation operations functions of the federal waste management system

    International Nuclear Information System (INIS)

    Shappert, L.B.; Klimas, M.J.

    1989-01-01

    This paper documents the functions that are necessary to operate the OCRWM transportation system. OCRWM's mission is to accept and transport spent fuel and high-level waste from waste generators to FWMS facilities. The emphasis is on transportation operations and assumes that all necessary facilities are in place and equipment designs and specifications are available to permit the system to operate properly. The information reported in this paper was developed for TOPO and is compatible with the draft revision of the Waste Management System Requirements and Description (SRD). 5 refs

  2. Process for assembly and transformation into Saccharomyces cerevisiae of a synthetic yeast artificial chromosome containing a multigene cassette to express enzymes that enhance xylose utilization designed for an automated pla

    Science.gov (United States)

    A yeast artificial chromosome (YAC) containing a multigene cassette for expression of enzymes that enhance xylose utilization (xylose isomerase [XI] and xylulokinase [XKS]) was constructed and transformed into Saccharomyces cerevisiae to demonstrate feasibility as a stable protein expression system ...

  3. Modeling and Evaluation of LTE in Intelligent Transportation Systems

    NARCIS (Netherlands)

    Trichias, K.; van den Berg, Hans Leo; de Jongh, J.; Litjens, R.; Dimitrova, D.C.; Brogle, M.; Braun, T.; Heijenk, Gerhard J.; Meratnia, Nirvana

    The term Intelligent Transportation Systems (ITS) refers to adding information and communications technology to transport infrastructure and ve- hicles. The IEEE 802.11p standard is considered the main candidate for com- munication within the context of ITS and it performs well for active safety use

  4. Automated transportation management system (ATMS) software project management plan (SPMP)

    Energy Technology Data Exchange (ETDEWEB)

    Weidert, R.S., Westinghouse Hanford

    1996-05-20

    The Automated Transportation Management System (ATMS) Software Project Management plan (SPMP) is the lead planning document governing the life cycle of the ATMS and its integration into the Transportation Information Network (TIN). This SPMP defines the project tasks, deliverables, and high level schedules involved in developing the client/server ATMS software.

  5. Applying intelligent transport systems to manage noise impacts

    NARCIS (Netherlands)

    Wilmink, I.R.; Vonk, T.

    2015-01-01

    This contribution discusses how traffic management, and many other measures that can be categorised as Intelligent Transport Systems (ITS, i.e. all traffic and transport measures that use ICT) can help reduce noise levels by influencing mobility choices and driving behaviour. Several examples of

  6. Multimodal schedule design for synchromodal freight transport systems

    NARCIS (Netherlands)

    B. Behdani (Behzad); Y. Fan (Yun); B.W. Wiegmans (Bart); R.A. Zuidwijk (Rob)

    2016-01-01

    textabstractIntermodal freight transport has been discussed for decades as an alternative to unimodal road transport. However, it still does not represent a significant portion of the total freight market. A new and promising possibility to improve the performance of freight systems is the

  7. Multimodal schedule design for synchromodal freight transport systems

    NARCIS (Netherlands)

    Behdani, Behzad; Fan, Yun; Wiegmans, Bart; Zuidwijk, Rob

    2016-01-01

    Intermodal freight transport has been discussed for decades as an alternative to unimodal road transport. However, it still does not represent a significant portion of the total freight market. A new and promising possibility to improve the performance of freight systems is the synchromodal

  8. The emerging role of transport systems in liver function tests

    NARCIS (Netherlands)

    Stieger, Bruno; Heger, Michal; de Graaf, Wilmar; Paumgartner, Gustav; van Gulik, Thomas

    2012-01-01

    Liver function tests are of critical importance for the management of patients with severe or terminal liver disease. They are also used as prognostic tools for planning liver resections. In recent years many transport systems have been identified that also transport substances employed in liver

  9. Chromatic correction for the final transport system

    International Nuclear Information System (INIS)

    Brown, K.L.; Peterson, J.M.

    1980-01-01

    The final transport and focusing of the heavy-ion beam onto the fusion pellet in vacuum is complicated by several non-linear effects - namely, chromatic (momentum dependent) effects, geometric aberrations, and space-charge forces. This paper gives an example of how the chromatic effects can be nullified, at least to second order. Whether third- or higher-order terms are important is not yet clear. Space-charge effects are important but are not considered here

  10. Transport and Dynamics in Toroidal Fusion Systems

    International Nuclear Information System (INIS)

    Sovinec, Carl

    2016-01-01

    The study entitled, 'Transport and Dynamics in Toroidal Fusion Systems,' (TDTFS) applied analytical theory and numerical computation to investigate topics of importance to confining plasma, the fourth state of matter, with magnetic fields. A central focus of the work is how non-thermal components of the ion particle distribution affect the 'sawtooth' collective oscillation in the core of the tokamak magnetic configuration. Previous experimental and analytical research had shown and described how the oscillation frequency decreases and amplitude increases, leading to 'monster' or 'giant' sawteeth, when the non-thermal component is increased by injecting particle beams or by exciting ions with imposed electromagnetic waves. The TDTFS study applied numerical computation to self-consistently simulate the interaction between macroscopic collective plasma dynamics and the non-thermal particles. The modeling used the NIMROD code [Sovinec, Glasser, Gianakon, et al., J. Comput. Phys. 195, 355 (2004)] with the energetic component represented by simulation particles [Kim, Parker, Sovinec, and the NIMROD Team, Comput. Phys. Commun. 164, 448 (2004)]. The computations found decreasing growth rates for the instability that drives the oscillations, but they were ultimately limited from achieving experimentally relevant parameters due to computational practicalities. Nonetheless, this effort provided valuable lessons for integrated simulation of macroscopic plasma dynamics. It also motivated an investigation of the applicability of fluid-based modeling to the ion temperature gradient instability, leading to the journal publication [Schnack, Cheng, Barnes, and Parker, Phys. Plasmas 20, 062106 (2013)]. Apart from the tokamak-specific topics, the TDTFS study also addressed topics in the basic physics of magnetized plasma and in the dynamics of the reversed-field pinch (RFP) configuration. The basic physics work contributed to a study of two

  11. Transport and Dynamics in Toroidal Fusion Systems

    Energy Technology Data Exchange (ETDEWEB)

    Sovinec, Carl [Univ. of Wisconsin, Madison, WI (United States)

    2016-09-07

    The study entitled, "Transport and Dynamics in Toroidal Fusion Systems," (TDTFS) applied analytical theory and numerical computation to investigate topics of importance to confining plasma, the fourth state of matter, with magnetic fields. A central focus of the work is how non-thermal components of the ion particle distribution affect the "sawtooth" collective oscillation in the core of the tokamak magnetic configuration. Previous experimental and analytical research had shown and described how the oscillation frequency decreases and amplitude increases, leading to "monster" or "giant" sawteeth, when the non-thermal component is increased by injecting particle beams or by exciting ions with imposed electromagnetic waves. The TDTFS study applied numerical computation to self-consistently simulate the interaction between macroscopic collective plasma dynamics and the non-thermal particles. The modeling used the NIMROD code [Sovinec, Glasser, Gianakon, et al., J. Comput. Phys. 195, 355 (2004)] with the energetic component represented by simulation particles [Kim, Parker, Sovinec, and the NIMROD Team, Comput. Phys. Commun. 164, 448 (2004)]. The computations found decreasing growth rates for the instability that drives the oscillations, but they were ultimately limited from achieving experimentally relevant parameters due to computational practicalities. Nonetheless, this effort provided valuable lessons for integrated simulation of macroscopic plasma dynamics. It also motivated an investigation of the applicability of fluid-based modeling to the ion temperature gradient instability, leading to the journal publication [Schnack, Cheng, Barnes, and Parker, Phys. Plasmas 20, 062106 (2013)]. Apart from the tokamak-specific topics, the TDTFS study also addressed topics in the basic physics of magnetized plasma and in the dynamics of the reversed-field pinch (RFP) configuration. The basic physics work contributed to a study of two-fluid effects on interchange dynamics, where

  12. Design of an Autonomous Transport System for Coastal Areas

    Directory of Open Access Journals (Sweden)

    Andrzej Lebkowski

    2018-03-01

    Full Text Available The article presents a project of an autonomous transport system that can be deployed in coastal waters, bays or between islands. Presented solutions and development trends in the transport of autonomous and unmanned units (ghost ships are presented. The structure of the control system of autonomous units is discussed together with the presentation of applied solutions in the field of artificial intelligence. The paper presents the concept of a transport system consisting of autonomous electric powered vessels designed to carry passengers, bikes, mopeds, motorcycles or passenger cars. The transport task is to be implemented in an optimal way, that is, most economically and at the same time as safe as possible. For this reason, the structure of the electric propulsion system that can be found on such units is shown. The results of simulation studies of autonomous system operation using simulator of marine navigational environment are presented.

  13. Permissible state permit/fee systems for radioactive materials transportation

    International Nuclear Information System (INIS)

    Friel, L.

    1987-01-01

    Many state permit/fee systems for radioactive materials transportation have been ruled inconsistent with federal law invalidated by the courts. As the date for repository operation, and its associated transportation, draws near, more states can be expected to adopt permit/fee systems. Examination of the U.S. Department of Transportation's advisory rulings and federal court cases on previous permit/fee systems gives general guidance on the type of permit/fee systems most likely to withstand challenges. Such a system would: have a simplified permit application with minimal information requirements; address a federally-defined class of hazardous or radioactive materials; allow access to all shipments conducted in compliance with federal law; charge a fee reasonably related to the costs imposed on the state by the transportation; and minimize the potential for re-directing shipments to other jurisdictions

  14. Solutions to Improve Person Transport System in the Pitesti City by Analyzing Public Transport vs. Private Transport

    Science.gov (United States)

    Mihaela, Istrate; Alexandru, Boroiu; Viorel, Nicolae; Ionel, Vieru

    2017-10-01

    One of the major problems facing the Pitesti city is the road congestion that occurs in the central area of the city during the peak hours. With all the measures taken in recent years - the widening of road arteries, increasing the number of parking spaces, the creation of overground road passages - it is obvious that the problem can only be solved by a new philosophy regarding urban mobility: it is no longer possible to continue through solutions to increase the accessibility of the central area of the city, but it is necessary, on the contrary, to promote a policy of discouraging the penetration of vehicles in the city center, coupled with a policy of improving the connection between urban public transport and county public transport. This new approach is also proposed in the new Urban Mobility Plan of Pitesti city, under development. The most convincing argument for the necessity of this new orientation in the Pitesti city mobility plan is based on the analysis of the current situation of passenger transport on the territory of Pitesti city: the analysis of “public transport versus private transport” reveals a very low occupancy rate for cars and the fact that the road surface required for a passenger (the dynamic area) is much higher in the case of private transport than in the case of public transport. Measurements of passenger flows and vehicle flows on the 6 penetration ways in the city have been made and the calculations clearly demonstrate the benefits of an urban public transport system connected by “transshipment buses” to be made at the edge of the city, to the county public transport system. In terms of inter-county transport, it will continue to be connected to the urban public transport system by existing bus Station, within the city: South Bus Station and North Bus Station. The usefulness of the paper is that it identifies the solutions for sustainable mobility in Pitesti city and proposes concrete solutions for the development of the

  15. Transportation System Risk Assessment on DOE Defense Program shipments

    International Nuclear Information System (INIS)

    Brumburgh, G.P.; Kimura, C.Y.; Alesso, H.P.; Prassinos, P.G.

    1992-01-01

    Substantial effort has been expended concerning the level of safety provided to persons, property, and the environment from the hazards associated with transporting radioactive material. This work provided an impetus for the Department of Energy to investigate the use of probabilistic risk assessment techniques to supplement the deterministic approach to transportation safety. The DOE recently decided to incorporate the methodologies associated with PRAs in the process for authorizing the transportation of nuclear components, special assemblies, and radioactive materials affiliated with the DOE Defense Program. Accordingly, the LLNL, sponsored by the DOE/AL, is tasked with developing a safety guide series to provide guidance to preparers performing a transportation system risk assessment

  16. Hygromycin B and apramycin antibiotic resistance cassettes for use in Campylobacter jejuni.

    Directory of Open Access Journals (Sweden)

    Andrew Cameron

    Full Text Available Campylobacter jejuni genetic manipulation is restricted by the limited number of antibiotic resistance cassettes available for use in this diarrheal pathogen. In this study, two antibiotic resistance cassettes were developed, encoding for hygromycin B and apramycin resistance, for use in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. First, the marker genes were successfully modified to allow for insertional mutagenesis or deletion of a gene-of-interest, and were bracketed with restriction sites for the facilitation of site-specific cloning. These hygromycin B and apramycin markers are encoded by plasmids pAC1H and pAC1A, respectively. We also modified an insertional gene-delivery vector to create pRRH and pRRA, containing the hygromycin B and apramycin resistance genes, and 3 unique restriction sites for the directional introduction of genes into the conserved multi-copy rRNA gene clusters of the C. jejuni chromosome. We determined the effective antibiotic concentrations required for selection, and established that no harmful effects or fitness costs were associated with carrying hygromycin B or apramycin resistance under standard C. jejuni laboratory conditions. Using these markers, the arylsulfatase reporter gene astA was deleted, and the ability to genetically complement the astA deletion using pRRH and pRRA for astA gene insertion was demonstrated. Furthermore, the relative levels of expression from the endogenous astA promoter were compared to that of polycistronic mRNA expression from the constitutive promoter upstream of the resistance gene. The development of additional antibiotic resistance cassettes for use in Campylobacter will enable multiple gene deletion and expression combinations as well as more in-depth study of multi-gene systems important for the survival and pathogenesis of this important bacterium.

  17. The WIPP transportation system -- ''Safer than any other''

    International Nuclear Information System (INIS)

    Ward, T.R.; Spooner, R.

    1991-01-01

    The Department of Energy (DOE) has developed an integrated transportation system to transport transuranic (TRU) waste from ten widely dispersed generator sites to the Waste Isolation Pilot Plant (WIPP). The system consists of a Type B container, a specially designed trailer, a lightweight tractor, the DOE TRANSCOM satellite-based vehicle tracking system, and uniquely qualified and highly trained drivers. The DOE has demonstrated that this system is ready to transport the TRU waste to the WIPP site efficiently and safely. Since the system was put in place in November 1988, it has been repeatedly upgraded and enhanced to incorporate additional safety measures. In June of 1989, the National Academy of Sciences (NAS) reviewed the transportation system and concluded that ''the system proposed for transportation of TRU waste to WIPP is safer than that employed for any other hazardous material in the United States today and will reduce risk to very low levels'' (emphasis added). The NAS conclusion was made before the DOE implemented the Enhanced Driver Training Course for carrier drivers. The challenge facing the DOE was to examine the transportation system objectively and determine what additional improvements could be made to further enhance safety

  18. Estimated routine radiation doses to transportation workers in alternative spent-fuel transportation systems

    International Nuclear Information System (INIS)

    Schneider, K.J.; Smith, R.I.; Daling, P.M.; Ross, W.A.; McNair, G.W.

    1988-01-01

    The federal system for the management of spent fuel and high-level radioactive waste includes the acceptance by the US Department of Energy (DOE) of the spent fuel or waste loaded in casks at the reactor or other waste generators, its transportation to a repository, and its handling and final emplacement in the repository. The DOE plans to implement a transportation system that is safe, secure, efficient, and cost-effective and will meet applicable regulatory safety and security requirements. The DOE commissioned the Pacific Northwest Laboratory (PNL) to develop estimates of the routine radiation doses that would result from the operation of a system postulated using current designs and practices. From that evaluation, PNL identified activities/operations that result in the higher fraction of doses, proposed conceptual alternatives that would effectively reduce such exposures, and evaluated the cost-effectiveness of such alternatives. The study is one of a series used in making overall system design and operational decisions in the development of the DOE's spent-fuel/high-level waste transportation system. This paper contains the highlights from the PNL study of the estimated radiation doses to the transportation workers in a postulated reference transportation system and potential alternatives to that system

  19. US Department of Energy Automated Transportation Management System

    International Nuclear Information System (INIS)

    Portsmouth, J.H.

    1994-01-01

    The U.S. Department of Energy (DOE) Transportation Management Division (TMD) is responsible for managing its various programs via a diverse combination of Government-Owned/Contractor-Operated facilities. TMD is seeking to update it automation capabilities in capturing and processing DOE transportation information. TMD's Transportation Information Network (TIN) is an attempt to bring together transportation management, shipment tracking, research activities and software products in various stages of development. The TMD's Automated Transportation Management System (ATMS) proposes to assist the DOE and its contractors in performing their daily transportation management activities and to assist the DOE Environmental Management Division in its waste management responsibilities throughout the DOE complex. The ATMS system will center about the storage, handling and documentation involved in the environmental clean-up of DOE sites. Waste shipments will be moved to approved Treatment, Storage and Disposal (TSD) facilities and/or nuclear material repositories. An additional investment in shipping samples to analytical laboratories also involves packaging and documentation according to all applicable U.S. Department of Transportation (DOT) or International Air Transport Association (IATA) regulations. The most immediate goal of effectively managing DOE transportation management functions during the 1990's is an increase in automation capabilities of the DOE and its contractors. Subject-matter experts from various DOE site locations will be brought together to develop and refine these capabilities through the maximum use of computer applications. A major part of this effort will be the identification of the most economical modes of transportation and enhanced management reporting capabilities for transportation analysis. The ATMS system will also provide for increased strategic and shipment analysis during the 1990's and beyond in support of the DOE environmental mission

  20. Los Alamos radiation transport code system on desktop computing platforms

    International Nuclear Information System (INIS)

    Briesmeister, J.F.; Brinkley, F.W.; Clark, B.A.; West, J.T.

    1990-01-01

    The Los Alamos Radiation Transport Code System (LARTCS) consists of state-of-the-art Monte Carlo and discrete ordinates transport codes and data libraries. These codes were originally developed many years ago and have undergone continual improvement. With a large initial effort and continued vigilance, the codes are easily portable from one type of hardware to another. The performance of scientific work-stations (SWS) has evolved to the point that such platforms can be used routinely to perform sophisticated radiation transport calculations. As the personal computer (PC) performance approaches that of the SWS, the hardware options for desk-top radiation transport calculations expands considerably. The current status of the radiation transport codes within the LARTCS is described: MCNP, SABRINA, LAHET, ONEDANT, TWODANT, TWOHEX, and ONELD. Specifically, the authors discuss hardware systems on which the codes run and present code performance comparisons for various machines

  1. Cesium transport data for HTGR systems

    International Nuclear Information System (INIS)

    Myers, B.F.; Bell, W.E.

    1979-09-01

    Cesium transport data on the release of cesium from HTGR fuel elements are reviewed and discussed. The data available through 1976 are treated. Equations, parameters, and associated variances describing the data are presented. The equations and parameters are in forms suitable for use in computer codes used to calculate the release of metallic fission products from HTGR fuel elements into the primary circuit. The data cover the following processes: (1) diffusion of cesium in fuel kernels and pyrocarbon, (2) sorption of cesium on fuel rod matrix material and on graphite, and (3) migration of cesium in graphite. The data are being confirmed and extended through work in progress

  2. Elements of transport and emplacement system

    International Nuclear Information System (INIS)

    1981-02-01

    This report, undertaken to review proposals for transport, handling and emplacement of high-level radioactive wastes in an underground repository, appropriate to the U.K. context, falls under the headings: basic design concepts; waste block size and configuration; self-shielded or partially shielded blocks; concept of emplacement in long boreholes; concept of emplacement in short boreholes; concept of emplacement in tunnels; methods of emplacement; stages of disposal; repository access by adit, incline or shaft; handling techniques within repository; conventional and radiological safety; costs; areas for further research and development. (U.K.)

  3. MINIX SYSTEM TRANSPORTATION TO CYGNUS COMPUTER

    OpenAIRE

    GUILHERMO ESTEBAN SOSA BELTRAN

    1988-01-01

    O sistema operacional MINIX é uma nova implementação do sistema UNIX, versão 7, feito para fins didáticos. Ele está formado por uma coleção de processos, estruturados em 4 niveis: administração de processos, processos básicos do sistema, processos servidores de memória e arquivos, e processos usuários. A presente dissertação descreve o transporte do sistema MINIX, do microcomputador IBM PC XT para o computador CYGNUS do laboratório de Sistema de Computação do D...

  4. Advanced Public Transportation Systems. Technical Assistance Brief 2

    Science.gov (United States)

    1993-01-01

    The Advanced Public Transportation Systems (APTS) Program requires evaluation : of pilot projects. This technical assistance brief discusses the guidelines set : for developing evaluation framework and methodology for local projects. 4p.

  5. Advanced public transportation system deployment in the United States

    Science.gov (United States)

    1999-01-01

    This report documents work performed under FTA's Advanced Public Transportation Systems (APTS) Program, a program structured to undertake research and development of innovative applications of advanced navigation, information, and communication techn...

  6. Advanced public transportation systems : the state of the art

    Science.gov (United States)

    1991-03-01

    This report documents one of the early initiatives of UMTA's Advanced Public Transportation Systems (APTS) Program, a program structured to undertake research and development of innovative applications of advanced navigation, information, and communi...

  7. Benefits assessment of advanced public transportation system technologies, update 2000

    Science.gov (United States)

    This report was performed under the Federal Transit Administration's (FTA) Advanced Public Transportation Systems (APTS) Program. This program focuses on the development and demonstration of innovative advanced navigation, information and communicati...

  8. The public transportation system security and emergency preparedness planning guide

    Science.gov (United States)

    2003-01-01

    Recent events have focused renewed attention on the vulnerability of the nation's critical infrastructure to major events, including terrorism. The Public Transportation System Security and Emergency Preparedness Planning Guide has been prepared to s...

  9. Intelligent transportation systems data compression using wavelet decomposition technique.

    Science.gov (United States)

    2009-12-01

    Intelligent Transportation Systems (ITS) generates massive amounts of traffic data, which posts : challenges for data storage, transmission and retrieval. Data compression and reconstruction technique plays an : important role in ITS data procession....

  10. Evaluation of Communication Alternatives for Intelligent Transportation Systems

    Science.gov (United States)

    2010-08-31

    The primary focus of this study involved developing a process for the evaluation of wireless technologies : for intelligent transportation systems, and for conducting experiments of potential wireless technologies : and topologies. Two wireless techn...

  11. Public transportation systems: Comparative analysis of quality of service

    Energy Technology Data Exchange (ETDEWEB)

    Negri, L.; Florio, L. (Rome Univ. La Sapienza (Italy). Facolta' di Ingegneria, Dipt. di Idraulica, Trasporti e Strade)

    The evaluation, choice and design of public transportation systems for urban areas requires, in addition to consolidated use parameters, other dimensions essential to supply-demand qualiflcative realignment, e.g.: 'door-to-door time' which allows system differentiation in terms of commercial velocity, frequency and length of route; technical productivity expressed as 'transport power' and 'specific transport power'; and 'system/service quality'. By the means of surveys, these factors can be incorporated into suitable mathematical models representing, in a complete and reliable way, all the functions which a given system actually delivers and those functions which it is expected to deliver by its users. This paper illustrates the application of these concepts in a comparative analysis of different public transportation options - light rail rapid transit, tram and bus networks.

  12. Advances in dynamic network modeling in complex transportation systems

    CERN Document Server

    Ukkusuri, Satish V

    2013-01-01

    This book focuses on the latest in dynamic network modeling, including route guidance and traffic control in transportation systems and other complex infrastructure networks. Covers dynamic traffic assignment, flow modeling, mobile sensor deployment and more.

  13. MODERN AND PERSPECTIVE ON-BOARD NTELLECTUAL TRANSPORT SYSTEMS

    Directory of Open Access Journals (Sweden)

    V. Topalidi

    2011-01-01

    Full Text Available For the purpose of systematization of modern and perspective intellectual transport sides systems (ITВS it is suggested their classification and planning of new ITВS for realization of safety control of vehicle technical condition.

  14. A Study of Transport Airplane Crash-Resistant Fuel Systems

    National Research Council Canada - National Science Library

    Robertson, S

    2002-01-01

    ...), of transport airplane crash-resistant fuel system (CRFS). The report covers the historical studies related to aircraft crash fires and fuel containment concepts undertaken by the FAA, NASA, and the U.S...

  15. An Artificial Neural Network Controller for Intelligent Transportation Systems Applications

    Science.gov (United States)

    1996-01-01

    An Autonomous Intelligent Cruise Control (AICC) has been designed using a feedforward artificial neural network, as an example for utilizing artificial neural networks for nonlinear control problems arising in intelligent transportation systems appli...

  16. Intelligent transportation systems business plan for Kentucky : final report.

    Science.gov (United States)

    2001-09-01

    This report presents a Business Plan for Intelligent Transportation Systems (ITS) in Kentucky. The purpose of the Business Plan is to define ITS projects that are planned for implementation from 2002 through 2007. The list of projects contained withi...

  17. Alternative Transportation System Demand Estimation for Federal Land Management Agencies.

    Science.gov (United States)

    2011-09-30

    Estimating travel demand for alternative transportation systems (ATS) is challenging in any context, but is even more daunting for Federal Land Management Agencies (FLMAs). Federal public land sites vary widely in their characteristics. Moreover, tra...

  18. Innovations in pricing of transportation systems : theory and practice.

    Science.gov (United States)

    2011-08-15

    This report summarizes results from the conference titled Innovations in Pricing of : Transportation Systems on May 12 14, 2010 at the Royal Plaza Hotel in Orlando, Florida. : The primary objective of the conference is to bring together pra...

  19. Developing a market-sensitive intelligent transportation systems educational program

    Science.gov (United States)

    1997-01-01

    Results of research undertaken to evaluate the educational needs of the emerging field of Intelligent Transportation Systems (ITSs) are presented, and whether course offerings in academic programs meet these needs is ascertained. A survey was conduct...

  20. MRS [monitored retrievable storage] to transportation system interfaces

    International Nuclear Information System (INIS)

    Row, T.H.; Croff, A.G.

    1987-01-01

    In March 1987, the US Department of Energy presented to Congress the proposal to construct and operate a facility for the monitored retrievable storage (MRS) of spent fuel at a site on the Clinch River in the Roane County portions of Oak Ridge. In discussing the MRS to Transportation System Interfaces, the authors provide a blending of the technical and institutional issues, for they do not believe the solutions to success of this enterprise lie wholly in one area. The authors cover: early chronology of the MRS; comparison of total-system life cycle cost estimates of the authorized system and improved-performance system (i.e., the system that includes a facility for MRS); transportation costs resulting from shipping, security and cask; assumptions for dedicated rail transport from MRS to repository; and significant results from the Total System Life Cycle Cost (TSLCC) analysis of the improved performance system. (AT)

  1. Towards a Hyperconnected Transportation Management System: Application to Blood Logistics

    OpenAIRE

    Schoen , Quentin; Lauras , Matthieu; Truptil , Sébastien; Fontanili , Franck; Anquetil , Anne-Ghislaine

    2016-01-01

    Part 1: Hyperconnectivity and Interoperation - Hyperconnected Systems; International audience; Internet of Things, connected devices, and other wireless sensors networks offer a number of new opportunities to manage transportation flows. This is particularly interesting for critical Supply Chains like Blood Supply Chains. In this research work, we investigate how such new technologies can enhance transportation system by better managing hazards and changes. By developing an event-driven decis...

  2. An environmentally sustainable transport system in Sweden. A scenario study

    Energy Technology Data Exchange (ETDEWEB)

    Brokking, P.; Emmelin, L.; Engstroem, M-G.; Nilsson, Jan-Evert; Eriksson, Gunnar; Wikberg, O.

    1997-02-01

    This is a short version of a scenario study concerning the possibilities to reach an Environmentally Sustainable Transport system in Sweden in a perspective of 30 years. The aim of the scenario study has been to describe one of several possible paths from today`s transport system to an environmentally adopted one. However, this does not imply that the task is to predict how such a transformation can be accomplished. The aim is rather to illustrate what such transformation require in the form of political decisions. The transformation of the transport system in to an environmentally adopted one, is primarily treated as a political problem, and a political perspective has accordingly been chosen for the study. In this English version of the scenario, the carbon dioxide problem is used to illuminate the many conflicts in goals and other problem that will attend an environmental adoption of the Swedish transport system, and to highlight the analytical points of departure for the scenario study. The analysis shows that it is possible to reach the national environmental goals that characterise, with given definitions, an environmentally sustainable transport system. However, this implies many severe political decisions over a long period of time, which in turn, implies a long term national consensus about the importance to reach the overall goal. Other results the scenario points out, is the risk that a policy focused on one sector leads to `solving` a problem by moving it outside systems limitations, and the limitations on a national environmental policy: Being able to count on assistance from other countries through an environmental adoption of the transport system in the European Union or globally, would drastically facilitate the environmental adoption of the Swedish transport system, through, among other things, a more rapid technological development. This indicates the necessity of promoting issues involving transportation and the environment in international

  3. DIRECTION OF MODERNIZATION OF THE ARCTIC MARINE TRANSPORTATION SYSTEM

    Directory of Open Access Journals (Sweden)

    N. I. Komkov

    2014-01-01

    Full Text Available The article deals with the modern processes of formation and development of the marine transportation system in the Russian Arctic, analyzes its problem areas and reserves growth. Shows the status and prospects of development of cargo specialized fl eet of ice-class and icebreakers. Particular attention is paid to infrastructure, port management, port The possibility of creating special economic zones. Systematized direction of modernization of the Arctic marine transportation system.

  4. Wind turbines application for energy savings in Gas transportation system

    OpenAIRE

    Mingaleeva, Renata

    2014-01-01

    The Thesis shows the perspectives of involving renewable energy resources into the energy balance of Russia, namely the use of wind energy for the purpose of energy supply for the objects of the Russian Gas transportation system. The methodology of the wind energy technical potential calculation is designed and the wind energy technical potential assessment for onshore and offshore zones of Russia is presented. The analysis of Russian Gas transportation system in terms of energy consumption i...

  5. Highly Scalable Trip Grouping for Large Scale Collective Transportation Systems

    DEFF Research Database (Denmark)

    Gidofalvi, Gyozo; Pedersen, Torben Bach; Risch, Tore

    2008-01-01

    Transportation-related problems, like road congestion, parking, and pollution, are increasing in most cities. In order to reduce traffic, recent work has proposed methods for vehicle sharing, for example for sharing cabs by grouping "closeby" cab requests and thus minimizing transportation cost...... and utilizing cab space. However, the methods published so far do not scale to large data volumes, which is necessary to facilitate large-scale collective transportation systems, e.g., ride-sharing systems for large cities. This paper presents highly scalable trip grouping algorithms, which generalize previous...

  6. A MULTI-AGENT SYSTEM FOR FOREST TRANSPORT ACTIVITY PLANNING

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Araújo Júnior

    2017-09-01

    Full Text Available This study aims to propose and implement a conceptual model of an intelligent system in a georeferenced environment to determine the design of forest transport fleets. For this, we used a multi-agent systems based tool, which is the subject of studies of distributed artificial intelligence. The proposed model considers the use of plantation mapping (stands and forest roads, as well as information about the different vehicle transport capacities. The system was designed to adapt itself to changes that occur during the forest transport operation process, such as the modification of demanded volume or the inclusion of route restrictions used by the vehicles. For its development, we used the Java programming language associated with the LPSolve library for the optimization calculation, the JADE platform to develop agents, and the ArcGis Runtime to determine the optimal transport routes. Five agents were modelled: the transporter, controller, router, loader and unloader agents. The model is able to determine the amount of trucks among the different vehicles available that meet the demand and availability of routes, with a focus on minimizing the total costs of timber transport. The system can also rearrange itself after the transportation routes change during the process.

  7. Flow and transport in hierarchically fractured systems

    International Nuclear Information System (INIS)

    Karasaki, K.

    1993-01-01

    Preliminary results indicate that flow in the saturated zone at Yucca Mountain is controlled by fractures. A current conceptual model assumes that the flow in the fracture system can be approximately by a three-dimensionally interconnected network of linear conduits. The overall flow system of rocks at Yucca Mountain is considered to consist of hierarchically structured heterogeneous fracture systems of multiple scales. A case study suggests that it is more appropriate to use the flow parameters of the large fracture system for predicting the first arrival time, rather than using the bulk average parameters of the total system

  8. Pneumatic transport systems for TRIGA reactors

    International Nuclear Information System (INIS)

    Bolton, John A.

    1970-01-01

    Main parameters and advantages of pneumatically operated systems, primarily those operated by gas pressure are discussed. The special irradiation ends for the TRIGA reactor are described. To give some idea of the complexity of some modern systems, the author presents the large system currently operating at the National Bureau of Standards in Washington. In this system, 13 stations are located throughout the radiochemistry laboratories and three irradiation ends are located in the reactor, which is a 14-megawatt unit. The system incorporates practically every fail-safe device possible, including ball valves located on all capsule lines entering the reactor area, designed to close automatically in the event of a reactor scram, and at that time capsules within the reactor would be diverted by means of switches located on the inside of the reactor wall. The whole system is under final control of a permission control panel located in the reactor control room. Many other safety accessories of the system are described

  9. Plasma transport simulation modeling for helical confinement systems

    International Nuclear Information System (INIS)

    Yamazaki, K.; Amano, T.

    1991-08-01

    New empirical and theoretical transport models for helical confinement systems are developed based on the neoclassical transport theory including the effect of radial electric field and multi-helicity magnetic components, and the drift wave turbulence transport for electrostatic and electromagnetic modes, or the anomalous semi-empirical transport. These electron thermal diffusivities are compared with CHS (Compact Helical System) experimental data, which indicates that the central transport coefficient of the ECH plasma agrees with the neoclassical axi-symmetric value and the transport outside the half radius is anomalous. On the other hand, the transport of NBI-heated plasmas is anomalous in the whole plasma region. This anomaly is not explained by the electrostatic drift wave turbulence models in these flat-density-profile discharges. For the detailed prediction of plasma parameters in LHD (Large Helical Device), 3-D(dimensional) equilibrium/1-D transport simulations including empirical or drift wave turbulence models are carried out, which suggests that the global confinement time of LHD is determined mainly by the electron anomalous transport near the plasma edge region rather than the helical ripple transport in the core region. Even if the ripple loss can be eliminated, the increase of the global confinement is 10%. However, the rise in the central ion temperature is more than 20%. If the anomalous loss can be reduced to the half level of the present scaling, like so-called 'H-mode' of the tokamak discharge, the neoclassical ripple loss through the ion channel becomes important even in the plasma core. The 5% radial inward shift of the plasma column with respect to the major radius is effective for improving plasma confinement and raising more than 50% of the fusion product by reducing this neoclassical asymmetric ion transport loss and increasing 10% in the plasma radius. (author)

  10. Plasma transport simulation modelling for helical confinement systems

    International Nuclear Information System (INIS)

    Yamazaki, K.; Amano, T.

    1992-01-01

    New empirical and theoretical transport models for helical confinement systems are developed on the basis of the neoclassical transport theory, including the effect of the radial electric field and of multi-helicity magnetic components as well as the drift wave turbulence transport for electrostatic and electromagnetic modes or the anomalous semi-empirical transport. These electron thermal diffusivities are compared with experimental data from the Compact Helical System which indicate that the central transport coefficient of a plasma with electron cyclotron heating agrees with neoclassical axisymmetric value and the transport outside the half-radius is anomalous. On the other hand, the transport of plasmas with neutral beam injection heating is anomalous in the whole plasma region. This anomaly is not explained by the electrostatic drift wave turbulence models in these discharges with flat density profiles. For a detailed prediction of the plasma parameters in the Large Helical Device (LHD), 3-D equilibrium/1-D transport simulations including empirical or drift wave turbulence models are performed which suggest that the global confinement time of the LHD is determined mainly by the electron anomalous transport in the plasma edge region rather than by the helical ripple transport in the core region. Even if the ripple loss can be eliminated, the increase in global confinement is 10%. However, the rise in the central ion temperature is more than 20%. If the anomalous loss can be reduced to half of the value used in the present scaling, as is the case in the H-mode of tokamak discharges, the neoclassical ripple loss through the ion channel becomes important even in the plasma core. The 5% radial inward shift of the plasma column with respect to the major radius improves the plasma confinement and increases the fusion product by more than 50% by reducing the neoclassical asymmetric ion transport loss and increasing the plasma radius (10%). (author). 32 refs, 7 figs

  11. Workshop on technology issues of superconducting Maglev transportation systems

    International Nuclear Information System (INIS)

    Wegrzyn, J.E.; Shaw, D.T.

    1991-01-01

    There exists a critical need in the United States to improve its ground transportation system. One suggested system that offers many advantages over the current transportation infrastructure is Maglev. Maglev represents the latest evolution in very high and speed ground transportation, where vehicles are magnetically levitated, guided, and propelled over elevated guideways at speeds of 300 miles per hour. Maglev is not a new concept but is, however, receiving renewed interest. The objective of this workshop was to further promote these interest by bringing together a small group of specialists in Maglev technology to discuss Maglev research needs and to identify key research issues to the development of a successful Maglev system. The workshop was organized into four sessions based on the following technical areas: Materials, Testing, and Shielding; Magnet Design and Cryogenic Systems; Propulsion and Levitation Systems; and, System Control and Integration

  12. Underground transportation and handling system for Pollux-casks

    International Nuclear Information System (INIS)

    Schrimpf, C.

    1988-01-01

    The concept for the underground transportation and handling system for Pollux-casks was optimized in a first phase by dividing the process in the repository up into the several transportation and manipulation steps. For each step, the possibilities were described and evaluated by means of a list of criteria (technical, safety and economical criteria). The following concept for the transportation and handling was developed: The casks are transported to the unloading area of the surface facilities by railway or truck. After removal of the transport protection, the entry control is performed. The cask is lifted from the vehicle and placed on a railbound transportation vehicle. This transport unit is transferred to the shaft and placed there ready for shaft hoisting. With the hoisting cage protruding, the transport unit is placed on the hoisting cage by means of a pushing-on device, locked, and then conveyed underground. After arrival on the emplacement level, the transport unit is pulled-off from the hoisting cage and taken over by a mine locomotive and transferred through the transportation and access drifts as far as to the emplacement site. There the locomotive pushed the rail transport vehicle into the emplacement drift, as far as to the designated emplacement position. At the emplacement position, the cask is again lifted by means of hoisting equipment. The rail transport vehicle is pulled out of the emplacement drift and returned to the surface for reloading. After deposition of the cask on the drift floor, the emplacement equipment is pulled back in order to give the operation space free for the slinger backfill truck. Within preceding tests two different backfilling techniques were investigated under realistic conditions: pneumatic backfilling and slinger backfilling. The slinger truck was found to be the most suitable for the designated purpose

  13. A Multilayer perspective for the analysis of urban transportation systems.

    Science.gov (United States)

    Aleta, Alberto; Meloni, Sandro; Moreno, Yamir

    2017-03-15

    Public urban mobility systems are composed by several transportation modes connected together. Most studies in urban mobility and planning often ignore the multi-layer nature of transportation systems considering only aggregated versions of this complex scenario. In this work we present a model for the representation of the transportation system of an entire city as a multiplex network. Using two different perspectives, one in which each line is a layer and one in which lines of the same transportation mode are grouped together, we study the interconnected structure of 9 different cities in Europe raging from small towns to mega-cities like London and Berlin highlighting their vulnerabilities and possible improvements. Finally, for the city of Zaragoza in Spain, we also consider data about service schedule and waiting times, which allow us to create a simple yet realistic model for urban mobility able to reproduce real-world facts and to test for network improvements.

  14. Toward a Shared Urban Transport System Ensuring Passengers & Goods Cohabitation

    Directory of Open Access Journals (Sweden)

    Anna Trentini

    2010-07-01

    Full Text Available The paper presents radical new urban transportation system concepts, potentially allowing changing the economic and environmental costs of passenger and freight transportation. The driver focuses on the concept of sharing, which means to make a joint use of transport resources, between passengers and goods flows. From a field observation of several existing solutions, an inductive reasoning enables us to move from a set of specific facts to establish an archetype for a radical new urban transportation system. Once the archetype defined, it is translated in real life through the example of the On Route proposal for London. The research frame of this paper is the ANR C-Goods (City Goods Operation Optimization using Decision support System project. Started in February 2009 the project involves four partners , and will end on 2011.

  15. Traffic Route Modelling and Assignment with Intelligent Transport System

    Directory of Open Access Journals (Sweden)

    Kunicina Nadezhda

    2014-12-01

    Full Text Available The development of signal transmitting environment for multimodal traffic control will enhance the integration of emergency and specialized transport routing tools in usual traffic control paradigms - it is one of the opportunities offered by modern intelligent traffic control systems. The improvement of effective electric power use in public transport system is an advantage of Intelligent Transport System (ITS. The research is connected with the improvement of on-line traffic control and adaptation of special traffic lighting alternatives by ITS. The assignment of the nearest appropriate transport will be done by passenger request, but unlike information system, the transport planning is done on demand. The task can be solved with the help of modern technical methods and equipment, as well as by applying control paradigms of the distributed systems. The problem is solved with the help of calculations hyper-graph and scheduling theory. The goal of the research is to develop methods, which support scheduling of the emergency transport, using high performance computing.

  16. Organelle-localized potassium transport systems in plants.

    Science.gov (United States)

    Hamamoto, Shin; Uozumi, Nobuyuki

    2014-05-15

    Some intracellular organelles found in eukaryotes such as plants have arisen through the endocytotic engulfment of prokaryotic cells. This accounts for the presence of plant membrane intrinsic proteins that have homologs in prokaryotic cells. Other organelles, such as those of the endomembrane system, are thought to have evolved through infolding of the plasma membrane. Acquisition of intracellular components (organelles) in the cells supplied additional functions for survival in various natural environments. The organelles are surrounded by biological membranes, which contain membrane-embedded K(+) transport systems allowing K(+) to move across the membrane. K(+) transport systems in plant organelles act coordinately with the plasma membrane intrinsic K(+) transport systems to maintain cytosolic K(+) concentrations. Since it is sometimes difficult to perform direct studies of organellar membrane proteins in plant cells, heterologous expression in yeast and Escherichia coli has been used to elucidate the function of plant vacuole K(+) channels and other membrane transporters. The vacuole is the largest organelle in plant cells; it has an important task in the K(+) homeostasis of the cytoplasm. The initial electrophysiological measurements of K(+) transport have categorized three classes of plant vacuolar cation channels, and since then molecular cloning approaches have led to the isolation of genes for a number of K(+) transport systems. Plants contain chloroplasts, derived from photoautotrophic cyanobacteria. A novel K(+) transport system has been isolated from cyanobacteria, which may add to our understanding of K(+) flux across the thylakoid membrane and the inner membrane of the chloroplast. This chapter will provide an overview of recent findings regarding plant organellar K(+) transport proteins. Copyright © 2014 Elsevier GmbH. All rights reserved.

  17. Concept of expert system for modal split in transportation planning

    Directory of Open Access Journals (Sweden)

    Popović Maja M.

    2006-01-01

    Full Text Available The objective of this paper is to develop a concept of expert system based on the survey of experts' opinions and their experience concerning relations in modal split, on the basis of parameters of transport system demand and transport supply, defined through PT travel time and city size, i.e. mean trip length. This expert system could be of use both to experts and less experienced planners who could apply the knowledge contained in this expert system for further improvement, on operational as well as on strategic level.

  18. Planning a transportation system for US Defense Transuranic waste

    International Nuclear Information System (INIS)

    Gilbert, K.V.; Hurley, J.D.; Smith, L.J.; McFadden, M.H.; Raudenbush, M.H.; Fedie, M.L.

    1983-05-01

    The development and planning of a transportation system for US Department of Energy (USDOE) Defense Transuranic (TRU) waste has required the talents and expertise of many people. Coordination activities, design activities, fabrication, research and development, operations, and transportation are but a few of the areas around which this system is built. Due to the large number of organizations, regulations and personalities the planning task becomes extremely complex. The intent of this paper is to discuss the steps taken in planning this system, to identify the various organizations around which this system is designed, and to discuss program progress to date, scheduling, and future plans. 9 figures, 1 table

  19. Modeling and Analysis of Modal Switching in Networked Transport Systems

    International Nuclear Information System (INIS)

    Hante, Falk M.; Leugering, Guenter; Seidman, Thomas I.

    2009-01-01

    We consider networked transport systems defined on directed graphs: the dynamics on the edges correspond to solutions of transport equations with space dimension one. In addition to the graph setting, a major consideration is the introduction and propagation of discontinuities in the solutions when the system may discontinuously switch modes, naturally or as a hybrid control. This kind of switching has been extensively studied for ordinary differential equations, but not much so far for systems governed by partial differential equations. In particular, we give well-posedness results for switching as a control, both in finite horizon open loop operation and as feedback based on sensor measurements in the system

  20. On the sample transport time of a pneumatic transfer system

    International Nuclear Information System (INIS)

    Kondo, Yoshihide

    1983-01-01

    The counts accumulated in measuring system are affected by the variations in transport time of the sample on cyclic activation experiments with a mechanical sample transfer system. In use of the pneumatic transfer system, which has been set up, the transport time is variable according to the differences as follows: The form, size and weight of samples, the pneumatic pressure and so on. Comprehending the relationships between the transpot time and these variable factors is essentially important to make experiments with this transfer system. (author)

  1. A Configurable, Object-Oriented, Transportation System Software Framework

    Energy Technology Data Exchange (ETDEWEB)

    KELLY,SUZANNE M.; MYRE,JOHN W.; PRICE,MARK H.; RUSSELL,ERIC D.; SCOTT,DAN W.

    2000-08-01

    The Transportation Surety Center, 6300, has been conducting continuing research into and development of information systems for the Configurable Transportation Security and Information Management System (CTSS) project, an Object-Oriented Framework approach that uses Component-Based Software Development to facilitate rapid deployment of new systems while improving software cost containment, development reliability, compatibility, and extensibility. The direction has been to develop a Fleet Management System (FMS) framework using object-oriented technology. The goal for the current development is to provide a software and hardware environment that will demonstrate and support object-oriented development commonly in the FMS Central Command Center and Vehicle domains.

  2. Gathering Information from Transport Systems for Processing in Supply Chains

    Science.gov (United States)

    Kodym, Oldřich; Unucka, Jakub

    2016-12-01

    Paper deals with complex system for processing information from means of transport acting as parts of train (rail or road). It focuses on automated information gathering using AutoID technology, information transmission via Internet of Things networks and information usage in information systems of logistic firms for support of selected processes on MES and ERP levels. Different kinds of gathered information from whole transport chain are discussed. Compliance with existing standards is mentioned. Security of information in full life cycle is integral part of presented system. Design of fully equipped system based on synthesized functional nodes is presented.

  3. Planning a transportation system for US defense transuranic waste

    International Nuclear Information System (INIS)

    Gilbert, K.V.; Hurley, J.D.; Smith, L.J.; McFadden, M.H.; Raudenbush, M.H.; Fedie, M.L.

    1983-01-01

    The development and planning of a transportation system for US Department of Energy (USDOE) Defense Transuranic (TRU) waste has required the talents and expertise of many people. Coordination activities, design activities, fabrication, research and development, operations, and transportation are but a few of the areas around which this system is built. Due to the large number of organizations, regulations and personalities the planning task becomes extremely complex. The intent of this paper is to discuss the steps taken in planning this system, to identify the various organizations around which this system is designed, and to discuss program progress to date, scheduling, and future plans

  4. Pulse thermal energy transport/storage system

    Science.gov (United States)

    Weislogel, Mark M.

    1992-07-07

    A pulse-thermal pump having a novel fluid flow wherein heat admitted to a closed system raises the pressure in a closed evaporator chamber while another interconnected evaporator chamber remains open. This creates a large pressure differential, and at a predetermined pressure the closed evaporator is opened and the opened evaporator is closed. This difference in pressure initiates fluid flow in the system.

  5. Relativistic Quantum Transport in Graphene Systems

    Science.gov (United States)

    2015-07-09

    dimensional Dirac material systems. 2 List of Publications 1. X. Ni, L. Huang, Y.-C. Lai, and L. M. Pecora, “Effect of chaos on relativistic quantum...development of relativistic quantum devices based on graphene or alternative two-dimensional Dirac material systems. In the project period, we studied

  6. The competitive advantage of a dual-transporter system.

    Science.gov (United States)

    Levy, Sagi; Kafri, Moshe; Carmi, Miri; Barkai, Naama

    2011-12-09

    Cells use transporters of different affinities to regulate nutrient influx. When nutrients are depleted, low-affinity transporters are replaced by high-affinity ones. High-affinity transporters are helpful when concentrations of nutrients are low, but the advantage of reducing their abundance when nutrients are abundant is less clear. When we eliminated such reduced production of the Saccharomyces cerevisiae high-affinity transporters for phosphate and zinc, the elapsed time from the initiation of the starvation program until the lack of nutrients limited growth was shortened, and recovery from starvation was delayed. The latter phenotype was rescued by constitutive activation of the starvation program. Dual-transporter systems appear to prolong preparation for starvation and to facilitate subsequent recovery, which may optimize sensing of nutrient depletion by integrating internal and external information about nutrient availability.

  7. Disinfection efficacy of an ultraviolet light on film cassettes for preventive of the nosocomial infection

    International Nuclear Information System (INIS)

    Kweon, Dae Cheol; Jeon, Yong Woong; Cho, Am

    2001-01-01

    The bacterial infection on film cassette contact surface was examined at the diagnostic radiology department of the S. hospital. The objective of this study was to assess the contamination level on film cassette contact surface as a predictor of patient prevention from nosocomial infection and for improvement of the hospital environment. The laboratory result was identified non-pathologic bacterial in the five different cassette size of the contact surface. Film cassettes were exposed to ultraviolet light for 1, 2 and 3 minutes. Ultraviolet light disinfection is proven suitable for bacterial. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. In addition education of nosocomial infection for radiographers will be required. In conclusion, ultraviolet is considered effective to irradiate bacterial. Additionally, two minutes are required to sterilize film cassettes

  8. Disinfection efficacy of an ultraviolet light on film cassettes for preventive of the nosocomial infection

    Energy Technology Data Exchange (ETDEWEB)

    Kweon, Dae Cheol [Seoul National Univ. Hospital, Seoul (Korea, Republic of); Jeon, Yong Woong; Cho, Am [Dongguk Univ., Seoul (Korea, Republic of)

    2001-06-01

    The bacterial infection on film cassette contact surface was examined at the diagnostic radiology department of the S. hospital. The objective of this study was to assess the contamination level on film cassette contact surface as a predictor of patient prevention from nosocomial infection and for improvement of the hospital environment. The laboratory result was identified non-pathologic bacterial in the five different cassette size of the contact surface. Film cassettes were exposed to ultraviolet light for 1, 2 and 3 minutes. Ultraviolet light disinfection is proven suitable for bacterial. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. In addition education of nosocomial infection for radiographers will be required. In conclusion, ultraviolet is considered effective to irradiate bacterial. Additionally, two minutes are required to sterilize film cassettes.

  9. Transportation ALARA analysis for a nuclear waste management system

    International Nuclear Information System (INIS)

    McNair, G. W.; Schneider, K.; Smith, R.I.; Ross, W.; Faletti, D.

    1988-01-01

    In planning for implementation of a safe and cost-effective transportation system, the Department of Energy (DOE) commissioned the Pacific Northwest Laboratory (PNL) to develop estimates of the radiation doses, both public and occupational, that would result from operation of a system postulated using current designs and practices. From that evaluation, PNL identified activities/operations that result in relatively high doses, proposed conceptual alternatives that would effectively reduce such exposures, and evaluated the cost-effectiveness of such alternatives. This study contains an analysis of routine operations and estimates of the public and worker radiation doses that would occur in a postulated generic reference spent fuel transportation system using both truck and rail modes. Total risks are not estimated (i.e., consideration of nonradiological or accident risks that will be the subject of future studies in the transportation systems study plan 9TSSP) are not included). The system encompasses spent fuel loading at the reactor, transportation of the fuel to and from a receiving and handling facility and unloading of the fuel at a repository. The analysis provides cost/dose trade-offs of the postulated reference system as well as selected potential alternatives to the transportation system

  10. TRANSPORT MECHANISM STUDIES OF CHITOSAN ELECTROLYTE SYSTEMS

    International Nuclear Information System (INIS)

    Navaratnam, S.; Ramesh, K.; Ramesh, S.; Sanusi, A.; Basirun, W.J.; Arof, A.K.

    2015-01-01

    ABSTRACT: Knowledge of ion-conduction mechanisms in polymers is important for designing better polymer electrolytes for electrochemical devices. In this work, chitosan-ethylene carbonate/propylene carbonate (chitosan-EC/PC) system with lithium acetate (LiCH 3 COO) and lithium triflate (LiCF 3 SO 3 ) as salts were prepared and characterized using electrochemical impedance spectroscopy to study the ion-conduction mechanism. It was found that the electrolyte system using LiCF 3 SO 3 salt had a higher ionic conductivity, greater dielectric constant and dielectric loss value compared to system using LiCH 3 COO at room temperature. Hence, it may be inferred that the system incorporated with LiCF 3 SO 3 dissociated more readily than LiCH 3 COO. Conductivity mechanism for the systems, 42 wt.% chitosan- 28 wt.% LiCF 3 SO 3 -30 wt.% EC/PC (CLT) and 42 wt.% chitosan-28 wt.% LiCH 3 COO-30 wt.% EC/PC (CLA) follows the overlapping large polaron tunneling (OLPT) model. Results show that the nature of anion size influences the ionic conduction of chitosan based polymer electrolytes. The conductivity values of the CLA system are found to be higher than that of CLT system at higher temperatures. This may be due to the vibration of bigger triflate anions would have hindered the lithium ion movements. FTIR results show that lithium ions can form complexation with polymer host which would provide a platform for ion hopping

  11. Fully Automated Data Collection Using PAM and the Development of PAM/SPACE Reversible Cassettes

    Science.gov (United States)

    Hiraki, Masahiko; Watanabe, Shokei; Chavas, Leonard M. G.; Yamada, Yusuke; Matsugaki, Naohiro; Igarashi, Noriyuki; Wakatsuki, Soichi; Fujihashi, Masahiro; Miki, Kunio; Baba, Seiki; Ueno, Go; Yamamoto, Masaki; Suzuki, Mamoru; Nakagawa, Atsushi; Watanabe, Nobuhisa; Tanaka, Isao

    2010-06-01

    To remotely control and automatically collect data in high-throughput X-ray data collection experiments, the Structural Biology Research Center at the Photon Factory (PF) developed and installed sample exchange robots PAM (PF Automated Mounting system) at PF macromolecular crystallography beamlines; BL-5A, BL-17A, AR-NW12A and AR-NE3A. We developed and installed software that manages the flow of the automated X-ray experiments; sample exchanges, loop-centering and X-ray diffraction data collection. The fully automated data collection function has been available since February 2009. To identify sample cassettes, PAM employs a two-dimensional bar code reader. New beamlines, BL-1A at the Photon Factory and BL32XU at SPring-8, are currently under construction as part of Targeted Proteins Research Program (TPRP) by the Ministry of Education, Culture, Sports, Science and Technology of Japan. However, different robots, PAM and SPACE (SPring-8 Precise Automatic Cryo-sample Exchanger), will be installed at BL-1A and BL32XU, respectively. For the convenience of the users of both facilities, pins and cassettes for PAM and SPACE are developed as part of the TPRP.

  12. CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors

    Directory of Open Access Journals (Sweden)

    Rasmus O. Bak

    2017-07-01

    Full Text Available The CRISPR/Cas9 system has recently been shown to facilitate high levels of precise genome editing using adeno-associated viral (AAV vectors to serve as donor template DNA during homologous recombination (HR. However, the maximum AAV packaging capacity of ∼4.5 kb limits the donor size. Here, we overcome this constraint by showing that two co-transduced AAV vectors can serve as donors during consecutive HR events for the integration of large transgenes. Importantly, the method involves a single-step procedure applicable to primary cells with relevance to therapeutic genome editing. We use the methodology in primary human T cells and CD34+ hematopoietic stem and progenitor cells to site-specifically integrate an expression cassette that, as a single donor vector, would otherwise amount to a total of 6.5 kb. This approach now provides an efficient way to integrate large transgene cassettes into the genomes of primary human cells using HR-mediated genome editing with AAV vectors.

  13. Transfer of radioactive materials in the fuel cycle. Transportation systems, transportation volume and radiation protection

    International Nuclear Information System (INIS)

    Schwarz, G.

    1997-01-01

    No other aspect of the carriage of hazardous goods has been provoking such long-lived concern in the general public and in the press during the last few years as the transport of spent nuclear fuels and high-level radioactive wastes to the storage facility at Gorleben. One reason for this controversy, besides clear-cut opposition in principal against such transfer activities, is the fact that there is an information gap, so that large parts of the population are not well informed about the relevant legal safety requirements and obligations governing such transports. The article therefore tries to fill this gap, presenting information on the number and necessity of transports of radioactive materials in the nuclear fuel cycle, the relevant scenarios, the transportation systems and packing and shielding requirements, as well as information on the radiological classification and hazardousness of waste forms. (Orig.) [de

  14. Modular transportable superconducting magnetic energy systems

    Science.gov (United States)

    Lieurance, Dennis; Kimball, Foster; Rix, Craig

    1995-04-01

    Design and cost studies were performed for the magnet components of mid-size (1-5 MWh), cold supported SMES systems using alternative configurations. The configurations studied included solenoid magnets, which required onsite assembly of the magnet system, and toroid and racetrack configurations which consisted of factory assembled modules. For each configuration, design concepts and cost information were developed for the major features of the magnet system including the conductor, electrical insulation, and structure. These studies showed that for mid-size systems, the costs of solenoid and toroid magnet configurations are comparable and that the specific configuration to be used for a given application should be based upon customer requirements such as limiting stray fields or minimizing risks in development or construction.

  15. Modular transportable superconducting magnetic Energy Systems

    Energy Technology Data Exchange (ETDEWEB)

    Lieurance, D.; Kimball, F.; Rix, C. [Martin Marietta Space Magnetics, San Diego, CA (United States)

    1994-12-31

    Design and cost studies were performed for the magnet components of mid-size (1-5 MWh), cold supported SMES systems using alternative configurations. The configurations studied included solenoid magnets, which required onsite assembly of the magnet system, and toroid and racetrack configurations which consisted of factory assembled modules. For each configuration, design concepts and cost information were developed for the major features of the magnet system including the conductor, electrical insulation, and structure. These studies showed that for mid-size systems, the costs of solenoid and toroid magnet configurations are comparable and that the specific configuration to be used for a given application should be based upon customer requirements such as limiting stray fields or minimizing risks in development or construction.

  16. Teaching transportation systems thinking concepts to undergraduates.

    Science.gov (United States)

    2013-05-01

    Systems thinking is thought by many academics to be a graduate level educational venture. : Many traditional educators in the engineering field argue that first a student should gain a : grounding in some traditional branch of engineering (civil and ...

  17. Geographic Names Information System (GNIS) Transportation Features

    Data.gov (United States)

    Department of Homeland Security — The Geographic Names Information System (GNIS) is the Federal standard for geographic nomenclature. The U.S. Geological Survey developed the GNIS for the U.S. Board...

  18. Earth land landing alternatives: Lunar transportation system

    Science.gov (United States)

    Meyerson, Robert

    1992-01-01

    The objectives of this study are as follows: (1) develop a landing option such that it is a viable trade option for future NASA missions; (2) provide NASA programs with solid technical support in the landing systems area; (3) develop the technical staff; and (4) advance the state of landing systems technology to apply to future NASA missions. All results are presented in viewgraph format.

  19. Recombinogenic engineering of conjugative plasmids with fluorescent marker cassettes

    DEFF Research Database (Denmark)

    Reisner, A.; Molin, Søren; Zechner, E.L.

    2002-01-01

    An efficient approach for the insertion of fluorescent marker genes with sequence specificity into conjugative plasmids in Escherichia coli is described. For this purpose, homologous recombination of linear double-stranded targeting DNA was mediated by the bacteriophage lambda recombination...... resistance genes and fluorescent markers. The choice of 5' non-homologous extensions in primer pairs used for amplifying the marker cassettes determines the site specificity of the targeting DNA. This methodology is applicable to the modification of all plasmids that replicate in E coli and is not restricted...

  20. Transportation of juvenile tambaqui (Colossoma macropomum in a closed system

    Directory of Open Access Journals (Sweden)

    L. C. Gomes

    Full Text Available The objective of this study was to investigate the effect of density, duration and the use of additives to the water during the transportation of juvenile tambaqui (Colossoma macropomum and use of this data to establish a safe transportation protocol for the species. The tested products and dosages were: salt (1000, 2000 and 3000 mg/L, gypsum (100, 300 and 500 mg/L and benzocaine (10, 20 and 30 mg/L. Fish were transported in closed systems (plastic bag at different densities and time periods of up to 24 h. Fish survival (FS and water quality parameters were monitored immediately after transportation. The remaining fish were kept in floating cages in order to evaluate mortality which occurred up to 96 h after transportation (S96. The best fish density, additives dosages and time period of the transportation was estimated with a general linear model. The effect of the condition factor on FS and S96 was also evaluated. As expected, FS and S96 were significantly related to time and density. FS but not S96, were also were significantly related to treatment. FS with gypsum treatment was not different from controls and FS with table salt and benzocaine treatments were significantly reduced. The condition factor was not related to either FS or S96. FS was inversely correlated with carbon dioxide concentration. It was concluded that the additives did not improve fish transportation survival. Linear models were developed to predict the best transportation densities as a function of time.

  1. Vacuum system control for the Heavy Ion Transport Line

    International Nuclear Information System (INIS)

    Stattel, P.; Feigenbaum, I.; Hseuh, H.C.; Robinson, T.; Skelton, R.; Wong, V.

    1987-01-01

    The Brookhaven AGS, 807 m in circumference, and the Tandem Van de Graaff are now joined together by a transport line, 600 m in length. This now allows heavy ions from the Tandem, up to fully stripped sulfur (M = 32) to be transported into the AGS and accelerated to 15 GeV/A. With the addition of a booster between the Tandem and the AGS in the near future, heavy ions such as gold (M = 200) can be accelerated to 30 Z/A GeV/A. This paper describes the HITL (Heavy Ion Transport Line) vacuum control system design and implementation

  2. Preliminary Transportation, Aging and Disposal Canister System Performance Specification

    International Nuclear Information System (INIS)

    C.A Kouts

    2006-01-01

    This document provides specifications for selected system components of the Transportation, Aging and Disposal (TAD) canister-based system. A list of system specified components and ancillary components are included in Section 1.2. The TAD canister, in conjunction with specialized overpacks will accomplish a number of functions in the management and disposal of spent nuclear fuel. Some of these functions will be accomplished at purchaser sites where commercial spent nuclear fuel (CSNF) is stored, and some will be performed within the Office of Civilian Radioactive Waste Management (OCRWM) transportation and disposal system. This document contains only those requirements unique to applications within Department of Energy's (DOE's) system. DOE recognizes that TAD canisters may have to perform similar functions at purchaser sites. Requirements to meet reactor functions, such as on-site dry storage, handling, and loading for transportation, are expected to be similar to commercially available canister-based systems. This document is intended to be referenced in the license application for the Monitored Geologic Repository (MGR). As such, the requirements cited herein are needed for TAD system use in OCRWM's disposal system. This document contains specifications for the TAD canister, transportation overpack and aging overpack. The remaining components and equipment that are unique to the OCRWM system or for similar purchaser applications will be supplied by others

  3. Expert system aids transport regulation users

    International Nuclear Information System (INIS)

    Cheshire, R.D.; Straw, R.J.

    1990-01-01

    During late 1984 the IAEA Regulations were identified as an area of application for an expert system adviser which could offer many advantages. Over the following year some simple tests were carried out to examine its feasibility, but TRANAID did not get underway until 1986 when British Nuclear Fuels (BNFL) Corporate Management services were engaged on the product. By this time a greater choice of suitable software, in the form of expert system shells, had become available. After a number of trial systems the shell Leonardo was finally adopted for the final system. In order for TRANAID to emulate the expert it was necessary to spend time extracting and documenting the expert knowledge. This was a matter of investigating how the regulations are used and was achieved by a series of meetings including opportunity for the computer specialists to interview the regulations experts. There are several benefits in having an expert system advisor in this area. It is useful to both experienced and inexperienced users of regulations. For those who are learning to use the regulations it is an excellent training aid. For those who know the regulations but use them infrequently it can save time and provide a valuable reassurance. The adviser has enabled the expert user's know how to be captured and to be made widely available to those with less experience. (author)

  4. Internal validation of the DNAscan/ANDE™ Rapid DNA Analysis™ platform and its associated PowerPlex® 16 high content DNA biochip cassette for use as an expert system with reference buccal swabs.

    Science.gov (United States)

    Moreno, Lilliana I; Brown, Alice L; Callaghan, Thomas F

    2017-07-01

    Rapid DNA platforms are fully integrated systems capable of producing and analyzing short tandem repeat (STR) profiles from reference sample buccal swabs in less than two hours. The technology requires minimal user interaction and experience making it possible for high quality profiles to be generated outside an accredited laboratory. The automated production of point of collection reference STR profiles could eliminate the time delay for shipment and analysis of arrestee samples at centralized laboratories. Furthermore, point of collection analysis would allow searching against profiles from unsolved crimes during the normal booking process once the infrastructure to immediately search the Combined DNA Index System (CODIS) database from the booking station is established. The DNAscan/ANDE™ Rapid DNA Analysis™ System developed by Network Biosystems was evaluated for robustness and reliability in the production of high quality reference STR profiles for database enrollment and searching applications. A total of 193 reference samples were assessed for concordance of the CODIS 13 loci. Studies to evaluate contamination, reproducibility, precision, stutter, peak height ratio, noise and sensitivity were also performed. The system proved to be robust, consistent and dependable. Results indicated an overall success rate of 75% for the 13 CODIS core loci and more importantly no incorrect calls were identified. The DNAscan/ANDE™ could be confidently used without human interaction in both laboratory and non-laboratory settings to generate reference profiles. Published by Elsevier B.V.

  5. Road Transportable Analytical Laboratory (RTAL) system

    International Nuclear Information System (INIS)

    1994-12-01

    The problem of groundwater contamination at a large number of industrial facilities is well known. Many US Army and Department of Energy (DOE) facilities share this problem of potentially contaminated water as a result of past disposal practices associated with military and energy source development activities. A wide range of contaminants are found at certain installations encompassing industrial pollutants and military-unique materials. The US Army Biomedical Research and Development Laboratory has been conducting research for a number of years on developing better means to determine the hazards associated with exposure to these types of complex mixtures. The methods involve the use of aquatic organisms together with in vitro mutagenicity assays and analytical chemistry in an integrated biological assessment of a specific site. Integrated Biological Assessment is an important development in the Army's continuing efforts to locate, clean and monitor sites contaminated as a result of military operations. This method provides meaningful hazard data regarding whether a test medium contains low levels of industrial or military-unique contaminants. This is an important advance in determining which sites are clean and which require remediation. It provides continuing monitoring of the effectiveness of remediation operations. Engineering Computer Opteconomics (ECO), Inc. was tasked, in a collaborative Army and DOE effort, to develop a transportable Integrated Biological Assessment Laboratory Complex. This multimodular Complex is designed to be taken into remote areas to provide the necessary long-term on-site research for determining hazards from low levels of contamination in the environment. Each module of the Complex is designed to be self-sufficient, to provide a safe environment for the operators, and a controlled environment for the test organisms and related critical chemical and biological analyses

  6. Transportation

    Science.gov (United States)

    2007-01-01

    Faculty ii INDUSTRY TRAVEL Domestic Assistant Deputy Under Secretary of Defense (Transportation Policy), Washington, DC Department of...developed between the railroad and trucking industries. Railroads: Today’s seven Class I freight railroad systems move 42% of the nation’s intercity ...has been successfully employed in London to reduce congestion and observed by this industry study during its travels . It is currently being

  7. The x-ray source application test cassette for radiation exposures at the OMEGA laser

    Energy Technology Data Exchange (ETDEWEB)

    Fournier, K. B.; Rekow, V.; Emig, J. [Lawrence Livermore National Laboratory, Livermore, California 94551 (United States); Fisher, J. H.; Newlander, C. D. [Fifth Gait Technologies, Inc., Huntsville, Alabama 35803 (United States); Horton, R. [Gray Research, Inc., Huntsville, Alabama 35806 (United States); Davis, J. [Defense Threat Reduction Agency, Fort Belvoir, Virginia 22060 (United States)

    2012-10-15

    We have designed a sample cassette that can be used to position up to six samples in the OMEGA laser chamber. The cassette accommodates round samples up to 38.1 mm (1.5{sup Double-Prime }) in diameter and square samples up to 27 mm on a side, any of which can be up to 12.7 mm thick. Smaller specimens are centered with spacers. The test cassette allows each sample to have a unique filter scheme, with multiple filter regions in front of each sample. This paper will present mechanical design considerations and operational aspects of the x-ray source application cassette.

  8. On wiping the interior walls of 37-mm closed-face cassettes: an OSHA perspective.

    Science.gov (United States)

    Hendricks, Warren; Stones, Fern; Lillquist, Dean

    2009-12-01

    As early as 1976, Occupational Safety and Health Administration (OSHA) methods for analyzing metal samples collected using 37-mm polystyrene closed-face cassettes specified that any loose dust be transferred from the cassette to the digestion vessel, that the cassette be rinsed, and that, if necessary, the cassette be wiped out to help ensure that all particles that enter the cassette are included along with the filter as part of the sample for analysis. OSHA analytical methods for metal analysis were recently revised to explicitly require cassette wiping for all metal samples. This change was based on policy that any material entering the collection device constitutes part of the sample and on OSHA Salt Lake Technical Center research showing that invisible residue on the cassette walls can significantly contribute to the total sample results reported. OSHA procedures are consistent with guidance given in the NIOSH Manual of Analytical Methods. This guidance concludes that internal deposits in sampling cassettes should be included in the analysis and that one way to accomplish this would be to wipe or wash the internal surfaces of the cassette and include the material along with the filter for analysis.

  9. Underground transport - the American system, a British dream

    Energy Technology Data Exchange (ETDEWEB)

    Cox, P N

    1987-01-01

    Between 6 July and 2 August 1985 a study tour of various collieries and equipment manufacturers in the USA was undertaken. The purpose of this tour was to study the transport of men and materials. The study tour consisted of four detailed studies of transport systems. These were augmented by manufacturers' visits comprising factory tours, technical presentations and underground equipment appraisals. An itinerary is given in Appendix A.

  10. Safety analysis report for packaging (onsite) sample pig transport system

    International Nuclear Information System (INIS)

    MCCOY, J.C.

    1999-01-01

    This Safety Analysis Report for Packaging (SARP) provides a technical evaluation of the Sample Pig Transport System as compared to the requirements of the U.S. Department of Energy, Richland Operations Office (RL) Order 5480.1, Change 1, Chapter III. The evaluation concludes that the package is acceptable for the onsite transport of Type B, fissile excepted radioactive materials when used in accordance with this document

  11. On nonequilibrium many-body systems III: nonlinear transport theory

    International Nuclear Information System (INIS)

    Luzzi, R.; Vasconcellos, A.R.; Algarte, A.C.S.

    1986-01-01

    A nonlinear transport theory for many-body systems arbitrarily away from equilibrium, based on the nonequilibrium statistical operator (NSO) method, is presented. Nonlinear transport equations for a basis set of dynamical quantities are derived using two equivalent treatments that may be considered far reaching generalizations of the Hilbert-Chapman-Enskog method and Mori's generalized Langevin equations method. The first case is considered in some detail and the general characteristics of the theory are discussed. (Author) [pt

  12. Transportation Infrastructure and the Asia-Pacific Food System

    OpenAIRE

    Armbruster, Walter J.; Coyle, William T.

    2005-01-01

    Adequate, well maintained transportation infrastructure is a critical element of the Pacific food system in addressing challenges of rapid urbanization. Policymakers must invest either in streamlining domestic supply chains and/or in facilitating food imports through market opening measures. Economic incentives, competitive transportation and logistic services, and policy reforms, both within the borders of individual economies as well as across the Asia-Pacific region, are also necessary. Im...

  13. Safety analysis report for packaging (onsite) sample pig transport system

    Energy Technology Data Exchange (ETDEWEB)

    MCCOY, J.C.

    1999-03-16

    This Safety Analysis Report for Packaging (SARP) provides a technical evaluation of the Sample Pig Transport System as compared to the requirements of the U.S. Department of Energy, Richland Operations Office (RL) Order 5480.1, Change 1, Chapter III. The evaluation concludes that the package is acceptable for the onsite transport of Type B, fissile excepted radioactive materials when used in accordance with this document.

  14. Optimal Operation of Interdependent Power Systems and Electrified Transportation Networks

    Directory of Open Access Journals (Sweden)

    M. Hadi Amini

    2018-01-01

    Full Text Available Electrified transportation and power systems are mutually coupled networks. In this paper, a novel framework is developed for interdependent power and transportation networks. Our approach constitutes solving an iterative least cost vehicle routing process, which utilizes the communication of electrified vehicles (EVs with competing charging stations, to exchange data such as electricity price, energy demand, and time of arrival. The EV routing problem is solved to minimize the total cost of travel using the Dijkstra algorithm with the input from EVs battery management system, electricity price from charging stations, powertrain component efficiencies and transportation network traffic conditions. Through the bidirectional communication of EVs with competing charging stations, EVs’ charging demand estimation is done much more accurately. Then the optimal power flow problem is solved for the power system, to find the locational marginal price at load buses where charging stations are connected. Finally, the electricity prices were communicated from the charging stations to the EVs, and the loop is closed. Locational electricity price acts as the shared parameter between the two optimization problems, i.e., optimal power flow and optimal routing problem. Electricity price depends on the power demand, which is affected by the charging of EVs. On the other hand, location of EV charging stations and their different pricing strategies might affect the routing decisions of the EVs. Our novel approach that combines the electrified transportation with power system operation, holds tremendous potential for solving electrified transportation issues and reducing energy costs. The effectiveness of the proposed approach is demonstrated using Shanghai transportation network and IEEE 9-bus test system. The results verify the cost-savings for both power system and transportation networks.

  15. City transport monitoring and routes optimal management system

    Directory of Open Access Journals (Sweden)

    V. Gargasas

    2008-06-01

    Full Text Available The article analyses the problem of further development of geographic informational systems with traffic monitoring channel (GIS-TMC in order to present the road users with effective information about the fastest (the shortest in respect of time routes and thus to improve the use of existing city transport infrastructure. To solve this task it is suggested to create dynamic (automatically updated in real time street passing duration base, for support of which a city transport monitoring system operating in real time is necessary, consisting of a network of sensors, a data collection communications system and a data processing system. In the article it is shown that to predict the street passing duration it is enough to measure speed of transport in the characteristic points of the street. Measurements of traffic density do not significantly improve accuracy of forecasting the street passing time. Analytical formulas are presented meant to forecast the street passing time.

  16. Non-rocket Earth-Moon transport system

    Science.gov (United States)

    Bolonkin, Alexander

    2003-06-01

    This paper proposes a new transportation system for travel between Earth and Moon. This transportation system uses mechanical energy transfer and requires only minimal energy, using an engine located on Earth. A cable directly connects a pole of the Earth through a drive station to the lunar surface_ The equation for an optimal equal stress cable for complex gravitational field of Earth-Moon has been derived that allows significantly lower cable masses. The required strength could be provided by cables constructed of carbon nanotubes or carbon whiskers. Some of the constraints on such a system are discussed.

  17. The Transportable Measurements Facility (TMF) System Description.

    Science.gov (United States)

    1980-05-23

    sites using different antennas, antenna/site characterization, ATCRBS-mode and DABS-mode processor evaluation, and DABS-based ATC and ATARS system...conditions met. 34 TABLE 3 TMF DATA RECORDED DURING EXPERIMENTS By Word Type 1) By Parameter Word No. of Bits Experiment Number 8 Physical Location Number of

  18. Design to nullify activity movement in heat transport systems

    International Nuclear Information System (INIS)

    Hemmings, R.L.; Barber, D.

    1975-01-01

    This article describes the methods by which designers can reduce the adverse effects of system corrosion and the resultant activation of the corrosion products in heat transport systems. The presentation will cover: a) choice of materials; b) assessment of the need of components; c) control of system chemistry; d) factors considered in sizing HTS purification systems; i) control of activation and fission products; ii) decontamination. (author)

  19. Transformations in Air Transportation Systems For the 21st Century

    Science.gov (United States)

    Holmes, Bruce J.

    2004-01-01

    Globally, our transportation systems face increasingly discomforting realities: certain of the legacy air and ground infrastructures of the 20th century will not satisfy our 21st century mobility needs. The consequence of inaction is diminished quality of life and economic opportunity for those nations unable to transform from the 20th to 21st century systems. Clearly, new thinking is required regarding business models that cater to consumers value of time, airspace architectures that enable those new business models, and technology strategies for innovating at the system-of-networks level. This lecture proposes a structured way of thinking about transformation from the legacy systems of the 20th century toward new systems for the 21st century. The comparison and contrast between the legacy systems of the 20th century and the transformed systems of the 21st century provides insights into the structure of transformation of air transportation. Where the legacy systems tend to be analog (versus digital), centralized (versus distributed), and scheduled (versus on-demand) for example, transformed 21st century systems become capable of scalability through technological, business, and policy innovations. Where air mobility in our legacy systems of the 20th century brought economic opportunity and quality of life to large service markets, transformed air mobility of the 21st century becomes more equitable available to ever-thinner and widely distributed populations. Several technological developments in the traditional aircraft disciplines as well as in communication, navigation, surveillance and information systems create new foundations for 21st thinking about air transportation. One of the technological developments of importance arises from complexity science and modern network theory. Scale-free (i.e., scalable) networks represent a promising concept space for modeling airspace system architectures, and for assessing network performance in terms of robustness

  20. The plasma membrane transport systems and adaptation to salinity.

    Science.gov (United States)

    Mansour, Mohamed Magdy F

    2014-11-15

    Salt stress represents one of the environmental challenges that drastically affect plant growth and yield. Evidence suggests that glycophytes and halophytes have a salt tolerance mechanisms working at the cellular level, and the plasma membrane (PM) is believed to be one facet of the cellular mechanisms. The responses of the PM transport proteins to salinity in contrasting species/cultivars were discussed. The review provides a comprehensive overview of the recent advances describing the crucial roles that the PM transport systems have in plant adaptation to salt. Several lines of evidence were presented to demonstrate the correlation between the PM transport proteins and adaptation of plants to high salinity. How alterations in these transport systems of the PM allow plants to cope with the salt stress was also addressed. Although inconsistencies exist in some of the information related to the responses of the PM transport proteins to salinity in different species/cultivars, their key roles in adaptation of plants to high salinity is obvious and evident, and cannot be precluded. Despite the promising results, detailed investigations at the cellular/molecular level are needed in some issues of the PM transport systems in response to salinity to further evaluate their implication in salt tolerance. Copyright © 2014 Elsevier GmbH. All rights reserved.