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Sample records for casei ssp rhamnosus

  1. Comparative Genomic and Functional Analysis of Lactobacillus casei and Lactobacillus rhamnosus Strains Marketed as Probiotics

    NARCIS (Netherlands)

    Douillard, F.P.; Ribbera, A.; Järvinen, H.M.; Kant, R.; Pietilä, T.E.; Randazzo, C.L.; Paulin, L.; Laine, P.K.; Caggia, C.; Ossowski, von I.; Reunanen, J.; Satokari, R.; Salminen, S.; Palva, A.; Vos, de W.M.

    2013-01-01

    Four Lactobacillus strains were isolated from marketed probiotic products, including L. rhamnosus strains from Vifit (Friesland Campina) and Idoform (Ferrosan) and L. casei strains from Actimel (Danone) and Yakult (Yakult Honsa Co.). Their genomes and phenotypes were characterized and compared in de

  2. Theoretical insight into the heat shock response (HSR) regulation in Lactobacillus casei and L. rhamnosus.

    Science.gov (United States)

    Rossi, Franca; Zotta, Teresa; Iacumin, Lucilla; Reale, Anna

    2016-08-01

    The understanding of the heat shock response (HSR) in lactobacilli from a regulatory point of view is still limited, though an increased knowledge on the regulation of this central stress response can lead to improvements in the exploitation of these health promoting microorganisms. Therefore the aim of this in silico study, that is the first to be carried out for members of the Lactobacillus genus, was predicting how HSR influences cell functions in the food associated and probiotic species Lactobacillus casei and Lactobacillus rhamnosus. To this purpose, thirteen whole genomes of these bacteria were analyzed to identify which genes involved in HSR are present. It was found that all the genomes share 25 HSR related genes, including those encoding protein repair systems, HSR repressors, HrcA and CtsR, and the positive regulators of HSR, alternative σ factors σ(32) and σ(24). Two genes encoding a σ(70)/σ(24) factor and a Lon protease, respectively, were found only in some genomes. The localization of the HSR regulators binding sites in genomes was analyzed in order to identify regulatory relationships driving HSR in these lactobacilli. It was observed that the binding site for the HrcA repressor is found upstream of the hrcA-grpE-dnaK-dnaJ and groES-groEL gene clusters, of two hsp genes, clpE, clpL and clpP, while the CtsR repressor binding site precedes the ctsR-clpC operon, clpB, clpE and clpP. Therefore the ClpE-ClpP protease complex is dually regulated by HrcA and CtsR. Consensus sequences for the promoters recognized by the HSR alternative σ factors were defined for L. casei and L. rhamnosus and were used in whole genome searches to identify the genes that are possibly regulated by these transcription factors and whose expression level is expected to increases in HSR. The results were validated by applying the same procedure of promoter consensus generation and whole genome search to an additional 11 species representative of the main Lactobacillus

  3. Cell-free supernatants from probiotic Lactobacillus casei and Lactobacillus rhamnosus GG decrease colon cancer cell invasion in vitro.

    Science.gov (United States)

    Escamilla, Juanita; Lane, Michelle A; Maitin, Vatsala

    2012-08-01

    Probiotics have been shown to have a preventative role in colorectal carcinogenesis but research concerning their prophylactic potential in the later stages of colorectal cancer, specifically metastasis is limited. This study explored the potential of cell-free supernatants (CFS) from 2 probiotic Lactobacillus sp., Lactobacillus casei and Lactobacillus rhamnosus GG, to inhibit colon cancer cell invasion by influencing matrix metalloproteinase-9 (MMP-9) activity and levels of the tight junction protein zona occludens-1 (ZO-1) in cultured metastatic human colorectal carcinoma cells. HCT-116 cells were treated with CFS from L. casei, L. rhamnosus, or Bacteroides thetaiotaomicron (a gut commensal); or with uninoculated bacterial growth media. Treatment with CFS from both Lactobacillus sp. decreased colorectal cell invasion but treatment with CFS from B. thetaiotaomicron did not. CFS from both Lactobacillus sp. decreased MMP-9 and increased ZO-1 protein levels. L. rhamnosus CFS also lowered MMP-9 activity. To begin elucidating the secreted bacterial factor conveying these responses, Lactobacillus sp. CFS were fractionated into defined molecular weight ranges and cell invasion assessed. Fractionation revealed that the inhibitory activity was contained primarily in the >100 kDa and 50-100 kDa fractions, suggesting the inhibitory compound may be a macromolecule such as a protein, nucleic acid, or a polysaccharide. PMID:22830611

  4. Screening Lactobacillus casei rhamnosus of soymilk starter with inhibitory effect%有抑菌功能的豆奶发酵剂Lactobacillus casei rhamnosus的选育

    Institute of Scientific and Technical Information of China (English)

    刘冬梅; 刘淑华; 李理; 梁世中

    2008-01-01

    从15个茵株中筛选出抑茵作用好的Lactobacillus casei rhamnosus LCR 6013,该茵能使豆奶均匀凝乳,在MILS中的培养上清液对金黄色葡萄球茵有极好的抑制作用,抑茵圈直径 25~26 mm.该菌株依次用Nisin溶液、酸溶液和NaCl溶液驯化后,抑茵作用有所增加.驯化后的LCIL 719W在豆奶中高密度生长并有良好的稳定性.

  5. The use of date waste for lactic acid production by a fed-batch culture using Lactobacillus casei subsp. rhamnosus

    Directory of Open Access Journals (Sweden)

    Aicha Nancib

    2015-09-01

    Full Text Available The production of lactic acid from date juice by Lactobacillus caseisubsp. rhamnosus in batch and fed-batch cultures has been investigated. The fed-batch culture system gave better results for lactic acid production and volumetric productivity. The aim of this work is to determine the effects of the feeding rate and the concentration of the feeding medium containing date juice glucose on the cell growth, the consumption of glucose and the lactic acid production by Lactobacillus casei subsp. rhamnosus in fed-batch cultures. For this study, two concentrations of the feeding medium (62 and 100 g/L of date juice glucose were tested at different feeding rates (18, 22, 33, 75 and 150 mL/h. The highest volumetric productivity (1.3 g/L.h and lactic acid yield (1.7 g/g were obtained at a feeding rate of 33 mL/h and a date juice glucose concentration of 62 g/L in the feeding medium. As a result, most of the date juice glucose was completely utilised (residual glucose 1 g/L, and a maximum lactic acid production level (89.2 g/L was obtained.

  6. Lactobacillus casei subsp.rhamnosus719对泡菜中亚硝酸盐的影舷%Effect of Lactobacillus casei subsp. rhamnosus 719 on Inhibition of Nitrites in Pickles

    Institute of Scientific and Technical Information of China (English)

    刘冬梅; 吴晖; 余以刚; 高建华

    2008-01-01

    为控制泡菜中的潜在致癌物--亚硝酸盐,将Lactobacillus casei subsp. rhamnosus 719(LCR 719)接种到泡菜中,进行发酵,制备出亚硝酸盐含量低的泡菜,并对泡菜发酵和贮藏过程中的亚硝酸盐含量、L-乳酸含量和酸度进行研究.采用盐酸萘乙二胺法测得接种LCR 719发酵120 h的泡菜中亚硝酸盐含量为2.214 mg/L,低于对照样的10.500 mg/L;用手性柱的高效液相法测得发酵24 h的泡菜中L-乳酸含量为13.74 g/L.结果表明,LCR 719在泡菜发酵过程中快速产生L-乳酸,从而抑制了亚硝酸盐的产生.

  7. Impact of different cryoprotectants on the survival of freeze-dried Lactobacillus rhamnosus and Lactobacillus casei/paracasei during long-term storage.

    Science.gov (United States)

    Jofré, A; Aymerich, T; Garriga, M

    2015-01-01

    The production of long shelf-life highly concentrated dried probiotic/starter cultures is of paramount importance for the food industry. The aim of the present study was to evaluate the protective effect of glucose, lactose, trehalose, and skim milk applied alone or combined upon the survival of potentially probiotic Lactobacillus rhamnosus CTC1679, Lactobacillus casei/paracasei CTC1677 and L. casei/paracasei CTC1678 during freeze-drying and after 39 weeks of storage at 4 and 22 °C. Immediately after freeze-drying, the percentage of survivors was very high (≥ 94%) and only slight differences were observed among strains and cryoprotectants. In contrast, during storage, survival in the dried state depended on the cryoprotectant, temperature and strain. For all the protectants assayed, the stability of the cultures was remarkably higher when stored under refrigeration (4 °C). Under these conditions, skim milk alone or supplemented with trehalose or lactose showed the best performance (reductions ≤ 0.9 log units after 39 weeks of storage). The lowest survival was observed during non-refrigerated storage and with glucose and glucose plus milk; no viable cells left at the end of the storage period. Thus, freeze-drying in the presence of appropriate cryoprotectants allows the production of long shelf-life highly concentrated dried cultures ready for incorporation in high numbers into food products as starter/potential probiotic cultures.

  8. Lactobacillus casei rhamnosus的抑菌作用及最佳抑菌作用发酵条件优化%Antimicrobial Activity of Lactobacillus casei rhamnosus and Optimization of Its Fermentation Conditions for Optimal Inhibitory Effects

    Institute of Scientific and Technical Information of China (English)

    刘冬梅; 吴晖; 李理; 梁世中

    2008-01-01

    为了研究Lactobacillus casei subsp.rhamnosusLCR 719产生抑菌作用的原因,应用牛津杯琼脂扩散法测定LCR 719在MRS上清液的抑菌作用.LCR 719在MRS中发酵时,醋酸先升后降,乳酸不断增加,乳酸和醋酸的摩尔浓度比从6h的0.28增加到60h的2.63.最佳抑菌作用的培养条件为:初始pH6.5, 吐温80 10.0ml/L,葡萄糖20.0g/L,培养温度37℃,培养时间为42h.该条件下的上清液对S.aureus抑菌圈直径达42mm,对枯草芽孢杆菌和肠炎沙门氏菌也有很好的抑菌效果,可降低E.coli及EHEC O157菌群密度,对酵母无抑制作用.结果表明,LCR 719是一种潜在的可用于生物防腐的保护性菌种.

  9. Study of cryoprotective agents during freeze-drying Lactobacillus casei subsp.rhamnosus 719%干酪乳杆菌鼠李糖亚种冻干保护剂的研究

    Institute of Scientific and Technical Information of China (English)

    邢蕾; 吴晖; 刘冬梅; 石英

    2009-01-01

    以存活率为指标,对干酪乳杆菌鼠李糖亚种(Lactobacillus casei subsp.rhamnosus 719)的菌体进行冻干保护研究,通过优化冻干保护剂,以降低冻干过程中的细胞损伤.结果表明,7种保护剂中,保护效果由大到小依次为海藻糖、脱脂奶粉、蔗糖、甘油、可溶性淀粉、硫酸锰、抗坏血酸;复配则以质量分数10%海藻糖+10%脱脂奶粉的组合为最佳,其菌体存活率达100%,冻干菌粉活菌数达1011 g-1.

  10. Synbiotic impact of tagatose on viability of Lactobacillus rhamnosus strain GG mediated by the phosphotransferase system (PTS).

    Science.gov (United States)

    Koh, Ji Hoon; Choi, Seung Hye; Park, Seung Won; Choi, Nag-Jin; Kim, Younghoon; Kim, Sae Hun

    2013-10-01

    Synbiotics, the combination of prebiotics and probiotics, has been shown to produce synergistic effects that promote gastrointestinal well-being of host. Tagatose is a low calorie food ingredient with putative health-promoting benefits. Herein, we investigated its synbiotic impact on the viability of Lactobacillus casei 01 and Lactobacillus rhamnosus strain GG and the potential mechanism involved. Tagatose, as a synbiotic substrate, enhanced the growth of L. casei 01 and L. rhamnosus strain GG compared to other prebiotics. Other gut-indigenous such as Clostridium spp. readily utilized fructooligosaccharide (FOS), the most widely used functional prebiotics, but not tagatose. Additionally, tagatose enhanced probiotic functions of L. casei 01 and L. rhamnosus strain GG by reinforcing their attachment on HT-29 intestine epithelial cells and enhancing their cholesterol-lowering activities. Whole transcriptome study and quantitative real-time polymerase chain reaction (qRT-PCR) test showed that the presence of tagatose in L. rhamnosus strain GG caused induction of a large number of genes associated with carbohydrate metabolism including the phosphotransferase system (PTS). Collectively, these results indicate the tagatose enhanced the growth of L. casei 01 and L. rhamnosus strain GG and their probiotic activities by activating tagatose-associated PTS networks. Importantly, this study highlights the potential application of tagatose and L. casei 01 and/or L. rhamnosus strain GG as a synbiotic partner in functional dairy foods (i.e. yogurt and cheese) and therapeutic dietary supplements.

  11. Synbiotic impact of tagatose on viability of Lactobacillus rhamnosus strain GG mediated by the phosphotransferase system (PTS).

    Science.gov (United States)

    Koh, Ji Hoon; Choi, Seung Hye; Park, Seung Won; Choi, Nag-Jin; Kim, Younghoon; Kim, Sae Hun

    2013-10-01

    Synbiotics, the combination of prebiotics and probiotics, has been shown to produce synergistic effects that promote gastrointestinal well-being of host. Tagatose is a low calorie food ingredient with putative health-promoting benefits. Herein, we investigated its synbiotic impact on the viability of Lactobacillus casei 01 and Lactobacillus rhamnosus strain GG and the potential mechanism involved. Tagatose, as a synbiotic substrate, enhanced the growth of L. casei 01 and L. rhamnosus strain GG compared to other prebiotics. Other gut-indigenous such as Clostridium spp. readily utilized fructooligosaccharide (FOS), the most widely used functional prebiotics, but not tagatose. Additionally, tagatose enhanced probiotic functions of L. casei 01 and L. rhamnosus strain GG by reinforcing their attachment on HT-29 intestine epithelial cells and enhancing their cholesterol-lowering activities. Whole transcriptome study and quantitative real-time polymerase chain reaction (qRT-PCR) test showed that the presence of tagatose in L. rhamnosus strain GG caused induction of a large number of genes associated with carbohydrate metabolism including the phosphotransferase system (PTS). Collectively, these results indicate the tagatose enhanced the growth of L. casei 01 and L. rhamnosus strain GG and their probiotic activities by activating tagatose-associated PTS networks. Importantly, this study highlights the potential application of tagatose and L. casei 01 and/or L. rhamnosus strain GG as a synbiotic partner in functional dairy foods (i.e. yogurt and cheese) and therapeutic dietary supplements. PMID:23764214

  12. The spxB gene as a target to identify Lactobacillus casei group species in cheese.

    Science.gov (United States)

    Savo Sardaro, Maria Luisa; Levante, Alessia; Bernini, Valentina; Gatti, Monica; Neviani, Erasmo; Lazzi, Camilla

    2016-10-01

    This study focused on the spxB gene, which encodes for pyruvate oxidase. The presence of spxB in the genome and its transcription could be a way to produce energy and allow bacterial growth during carbohydrate starvation. In addition, the activity of pyruvate oxidase, which produces hydrogen peroxide, could be a mechanism for interspecies competition. Because this gene seems to provide advantages for the encoding species for adaptation in complex ecosystems, we studied spxB in a large set of cheese isolates belonging to the Lactobacillus casei group. Through this study, we demonstrated that this gene is widely found in the genomes of members of the L. casei group and shows variability useful for taxonomic studies. In particular, the HRM analysis method allowed for a specific discrimination between Lactobacillus rhamnosus, Lactobacillus paracasei and L. casei. Regarding the coding region, the spxB functionality in cheese was shown for the first time by real-time PCR, and by exploiting the heterogeneity between the L. casei group species, we identified the bacterial communities encoding the spxB gene in this ecosystem. This study allowed for monitoring of the active bacterial community involved in different stages of ripening by following the POX pathway. PMID:27375244

  13. Functional Analysis of the Lactobacillus casei BL23 Sortases

    Science.gov (United States)

    Muñoz-Provencio, Diego; Rodríguez-Díaz, Jesús; Collado, María Carmen; Langella, Philippe; Bermúdez-Humarán, Luis G.

    2012-01-01

    Sortases are a class of enzymes that anchor surface proteins to the cell wall of Gram-positive bacteria. Lactobacillus casei BL23 harbors four sortase genes, two belonging to class A (srtA1 and srtA2) and two belonging to class C (srtC1 and srtC2). Class C sortases were clustered with genes encoding their putative substrates that were homologous to the SpaEFG and SpaCBA proteins that encode mucus adhesive pili in Lactobacillus rhamnosus GG. Twenty-three genes encoding putative sortase substrates were identified in the L. casei BL23 genome with unknown (35%), enzymatic (30%), or adhesion-related (35%) functions. Strains disrupted in srtA1, srtA2, srtC1, and srtC2 and an srtA1 srtA2 double mutant were constructed. The transcription of all four sortase encoding genes was detected, but only the mutation of srtA1 resulted in a decrease in bacterial surface hydrophobicity. The β-N-acetyl-glucosaminidase and cell wall proteinase activities of whole cells diminished in the srtA1 mutant and, to a greater extent, in the srtA1 srtA2 double mutant. Cell wall anchoring of the staphylococcal NucA reporter protein fused to a cell wall sorting sequence was also affected in the srtA mutants, and the percentages of adhesion to Caco-2 and HT-29 intestinal epithelial cells were reduced for the srtA1 srtA2 strain. Mutations in srtC1 or srtC2 result in an undetectable phenotype. Together, these results suggest that SrtA1 is the housekeeping sortase in L. casei BL23 and SrtA2 would carry out redundant or complementary functions that become evident when SrtA1 activity is absent. PMID:23042174

  14. Exopolysaccharides production in Lactobacillus bulgaricus and Lactobacillus casei exploiting microfiltration.

    Science.gov (United States)

    Schiraldi, C; Valli, V; Molinaro, A; Cartenì, M; De Rosa, M

    2006-05-01

    The physiology of Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus casei, extensively used in the dairy industry, was studied in order to evaluate key parameters in the synthesis of exopolysaccharides and to improve their production through novel fermentation processes. Selected strains were studied in shake flasks and in fermentor experiments using glucose and lactose as main carbon sources and bacto casitone as the only complex component, in a temperature range between 35 and 42 degrees C. The production of exopolysaccharides was monitored and correlated to the growth conditions using both a colorimetric assay and chromatographic methods. Fermentor experiments in batch mode yielded 100 mg l(-1) of EPS from L. bulgaricus and 350 mg l(-1) from L. casei. Moreover, the use of a microfiltration (MF) bioreactor resulted in exopolysaccharides (EPS) concentrations threefold and sixfold those of batch experiments, respectively. The monosaccharidic composition of the two analyzed polymers differed from those previously reported. The optimization of the production of EPSs using the MF fermentation strategy could permit the use of these molecules produced by generally recognised as safe (GRAS) microorganisms in the place of other polysaccharides in the food industry.

  15. Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells

    Institute of Scientific and Technical Information of China (English)

    Anna Oksaharju; Matti Kankainen; Riina A Kekkonen; Ken A Lindstedt; Petri T Kovanen; Riitta Korpela; Minja Miettinen

    2011-01-01

    AIM: To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS: Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L.rhamnosus ) GG (LGG(R)), L. rhamnosus Lc705 (Lc705),Propionibacterium freudenreichii ssp. shermanii JS (PJS) and Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination for 3 or 24 h, and were subjected to global microarray analysis using an Affymetrix GeneChip(R) Human Genome U133 Plus 2.0 Array. The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS: LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G, respectively)and histamine H4 receptor. LGG, Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation, and on several genes that encoded proteins with a pro-inflammatory impact, such as interleukin (IL)-8 and tumour necrosis factor alpha. Also genes that encoded proteins with anti-inflammatory functions, such as IL-10, were upregulated. CONCLUSION: Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes, and by inducing a pro-inflammatory response.

  16. Characterization of the Lactobacillus casei group based on the profiling of ribosomal proteins coded in S10-spc-alpha operons as observed by MALDI-TOF MS.

    Science.gov (United States)

    Sato, Hiroaki; Torimura, Masaki; Kitahara, Maki; Ohkuma, Moriya; Hotta, Yudai; Tamura, Hiroto

    2012-10-01

    The taxonomy of the members of the Lactobacillus casei group is complicated because of their phylogenetic similarity and controversial nomenclatural status. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of ribosomal proteins coded in the S10-spc-alpha operon, termed S10-GERMS, was applied in order to classify 33 sample strains belonging to the L. casei group. A total of 14 types of ribosomal protein genes coded in the operon were first sequenced from four type strains of the L. casei group (L. casei JCM 1134(T), L. paracasei subsp. paracasei JCM 8130(T), L. paracasei subsp. tolerans JCM 1171(T), and L. rhamnosus JCM 1136(T)) together with L. casei JCM 11302, which is the former type strain of 'L. zeae'. The theoretical masses of the 14 types of ribosomal proteins used as biomarkers were classified into five types and compiled into a ribosomal protein database. The observed ribosomal proteins of each strain, identified by MALDI-TOF MS, were categorized into types based on their masses, summarized as ribosomal protein profiles, and they were used to construct a phylogenetic tree. The 33 sample strains, together with seven genome-sequenced strains, could be classified into four major clusters, which coincided precisely with the taxa of the (sub)species within the L. casei group. Three "ancient" strains, identified as L. acidophilus and L. casei, were correctly re-identified as L. paracasei subsp. paracasei by S10-GERMS. S10-GERMS would thus appear to be a powerful tool for phylogenetic characterization, with considerable potential for management of culture collections.

  17. Immunomodulatory effect of Lactobacillus rhamnosus GG from

    Directory of Open Access Journals (Sweden)

    Stjepan Mahnet

    2004-01-01

    Full Text Available Various immune responses have been influenced by probiotics and these immunomodulatory effects have been proposed for several potential applications such as the prevention of infectious diarrhoea, alleviation of hypersensitivity reactions and tumour suppression. The new probiotic product from LURA d. d., low fat fresh cheese "BioAktiv LGG", contains well-known probiotic strain Lactobacillus rhamnosus GG (LGG. The viable count of L. rhamnosus GG remained stable and has been 1.3x108 CFU/g of low fat fresh cheese "BioAktiv LGG" during storage of 16 days at 4 0C. The effect of L. rhamnosus GG from this product on the immune response, as an importantpoint in relation to immunomodulation, and its survival/residence time in the gastrointestinal tract in Swiss albino mice was also studied. During the feeding of mice with L. rhamnosus GG, with a daily dose of 2.2x1010 cells, the number of lactobacilli in faeces was increased and reduction of enterobacteria and sulphite-reducing clostridia was observed. The similar results were obtained in homogenates of large intestine of mice in the 1st and in the 14th day after feeding with L. rhamnosus GG, which pointed out the adherence ability of examined strain. Furthermore, the oral immunization of Swiss albino mice was performed with a dose of 2.2x1010 L. rhamnosus GG cells per day, for eight consecutive days. The blood samples were collected on the 4th, 8th, 10th, 14th and 21st day after the 1st immunization. The sera of mice were tested for total and specific anti-lactobacilli IgA, IgG and IgM antibodies by ELISA assay. L. rhamnosus GG slightly stimulated the total humoral immune response but didn't evoke reaction against itself, which confirms its probiotic properties and make new probiotic product, low fat fresh cheese "BioAktiv LGG", as good carrier for this probiotic strain.

  18. High resolution melting analysis (HRM) as a new tool for the identification of species belonging to the Lactobacillus casei group and comparison with species-specific PCRs and multiplex PCR.

    Science.gov (United States)

    Iacumin, Lucilla; Ginaldi, Federica; Manzano, Marisa; Anastasi, Veronica; Reale, Anna; Zotta, Teresa; Rossi, Franca; Coppola, Raffaele; Comi, Giuseppe

    2015-04-01

    The correct identification and characterisation of bacteria is essential for several reasons: the classification of lactic acid bacteria (LAB) has changed significantly over the years, and it is important to distinguish and define them correctly, according to the current nomenclature, avoiding problems in the interpretation of literature, as well as mislabelling when probiotic are used in food products. In this study, species-specific PCR and HRM (high-resolution melting) analysis were developed to identify strains belonging to the Lactobacillus casei group and to classify them into L. casei, Lactobacillus paracasei and Lactobacillus rhamnosus. HRM analysis confirmed to be a potent, simple, fast and economic tool for microbial identification. In particular, 201 strains, collected from International collections and attributed to the L. casei group, were examined using these techniques and the results were compared with consolidated molecular methods, already published. Seven of the tested strains don't belong to the L. casei group. Among the remaining 194 strains, 6 showed inconsistent results, leaving identification undetermined. All the applied techniques were congruent for the identification of the vast majority of the tested strains (188). Notably, for 46 of the strains, the identification differed from the previous attribution. PMID:25475306

  19. Futile xylitol cycle in Lactobacillus casei.

    OpenAIRE

    Hausman, S Z; Thompson, J; London, J

    1984-01-01

    A futile xylitol cycle appears to be responsible for xylitol-mediated inhibition of growth of Lactobacillus casei Cl-16 at the expense of ribitol. The gratuitously induced xylitol-specific phosphoenolpyruvate-dependent phosphotransferase accumulates the pentitol as xylitol-5-phosphate, a phosphatase cleaves the latter, and an export system expels the xylitol. Operation of the cycle rapidly dissipates the ribitol-5-phosphate pool (and ultimately the energy supply of the cell), thereby producin...

  20. Reducing the Bitterness of Tuna (Euthynnus pelamis) Dark Meat with Lactobacillus casei subsp. casei ATCC 393

    OpenAIRE

    Bertoldi, Fabiano Cleber; Ernani S. Sant’Anna; Luiz H. Beirão

    2004-01-01

    During the process of canning tuna fish, considerable amounts of dark tuna meat are left over because of its bitterness, which are then used in the production of animal food. Fermentation with Lactobacillus casei subsp. casei ATCC 393 was used as an alternative to reduce this bitter taste. Samples of meat were prepared, vacuum packed and then stored at –18 °C. The frozen dark meat was used immediately after defrosting and the experiment was carried out with 2 and 4 % of NaCl with the addition...

  1. The effect of calcium ions on adhesion and competitive exclusion of Lactobacillus ssp. and E. coli O138

    DEFF Research Database (Denmark)

    Larsen, Nadja; Nissen, Peter; Willats, William G.T.

    2007-01-01

    The adhesion abilities of 11 strains of Lactobacillus were determined in vitro using the IPEC-J2 cell line as a model system. Bacteria cultures included the probiotic strains L. rhamnosus GG, L. reuteri ATCC 55730, L. johnsonii NCC 533 and L. reuteri DSM 12246, and new isolates of Lactobacillus ssp....... Adhesion was quantified by scintillation counting of radiolabelled bound bacteria. The highest adhesion of 38%, was determined for L. reuteri DSM 12246 followed by L. plantarum Q47 with an adhesion level of 24%. Other strains showed moderate to low binding of less than 16%. Competitive adhesion experiments...... on IPEC-J2 cells demonstrated that strongly adhesive strains, as L. reuteri DSM 12246 and L. plantarum Q47, significantly reduced the attachment of the less adhesive strains, such as L. rhamnosus GG and L. johnsonii NCC 533, both under condition of co-incubation and in displacement assays, indicating...

  2. The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG

    Directory of Open Access Journals (Sweden)

    Lebeer Sarah

    2012-02-01

    Full Text Available Abstract Background Although the occurrence, biosynthesis and possible functions of glycoproteins are increasingly documented for pathogens, glycoproteins are not yet widely described in probiotic bacteria. Nevertheless, knowledge of protein glycosylation holds important potential for better understanding specific glycan-mediated interactions of probiotics and for glycoengineering in food-grade microbes. Results Here, we provide evidence that the major secreted protein Msp1/p75 of the probiotic Lactobacillus rhamnosus GG is glycosylated. Msp1 was shown to stain positive with periodic-acid Schiff staining, to be susceptible to chemical deglycosylation, and to bind with the mannose-specific Concanavalin A (ConA lectin. Recombinant expression in Escherichia coli resulted in a significant reduction in molecular mass, loss of ConA reactivity and increased sensitivity towards pronase E and proteinase K. Mass spectrometry showed that Msp1 is O-glycosylated and identified a glycopeptide TVETPSSA (amino acids 101-108 bearing hexoses presumably linked to the serine residues. Interestingly, these serine residues are not present in the homologous protein of several Lactobacillus casei strains tested, which also did not bind to ConA. The role of the glycan substitutions in known functions of Msp1 was also investigated. Glycosylation did not seem to impact significantly on the peptidoglycan hydrolase activity of Msp1. In addition, the glycan chain appeared not to be required for the activation of Akt signaling in intestinal epithelial cells by Msp1. On the other hand, examination of different cell extracts showed that Msp1 is a glycosylated protein in the supernatant, but not in the cell wall and cytosol fraction, suggesting a link between glycosylation and secretion of this protein. Conclusions In this study we have provided the first evidence of protein O-glycosylation in the probiotic L rhamnosus GG. The major secreted protein Msp1 is glycosylated with Con

  3. Brevibacterium casei isolated as a cause of relapsing peritonitis

    OpenAIRE

    Althaf, Mohammed Mahdi; Abdelsalam, Mohamed Said; Alsunaid, Mohammed Sunaid; Hussein, Maged Hassan

    2014-01-01

    We report a case of relapsing peritonitis in a 33-year-old woman on automated peritoneal dialysis. End-stage renal disease was secondary to systemic lupus erythematosus complicated with lupus nephritis. The organism isolated was Brevibacterium casei that was not readily identified, delaying appropriate management with an extended antibiotic course. Definite management of B casei peritonitis was peritoneal dialysis catheter removal.

  4. Spontaneous release of bacteriophage particles by Lactobacillus rhamnosus pen.

    Science.gov (United States)

    Jarocki, Piotr; Podleśny, Marcin; Pawelec, Jarosław; Malinowska, Agata; Kowalczyk, Sylwia; Targoński, Zdzisław

    2013-03-01

    The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.

  5. Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation

    Directory of Open Access Journals (Sweden)

    Ayşegül Mendi

    2016-02-01

    Full Text Available ABSTRACT An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression. Objective The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs, including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation. Material and Methods G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis. Results CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01. Conclusions These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.

  6. Stress responses in probiotic Lactobacillus casei.

    Science.gov (United States)

    Hosseini Nezhad, Marzieh; Hussain, Malik Altaf; Britz, Margaret Lorraine

    2015-01-01

    Survival in harsh environments is critical to both the industrial performance of lactic acid bacteria (LAB) and their competitiveness in complex microbial ecologies. Among the LAB, members of the Lactobacillus casei group have industrial applications as acid-producing starter cultures for milk fermentations and as specialty cultures for the intensification and acceleration of flavor development in certain bacterial-ripened cheese varieties. They are amongst the most common organisms in the gastrointestinal (GI) tract of humans and other animals, and have the potential to function as probiotics. Whether used in industrial or probiotic applications, environmental stresses will affect the physiological status and properties of cells, including altering their functionality and biochemistry. Understanding the mechanisms of how LAB cope with different environments is of great biotechnological importance, from both a fundamental and applied perspective: hence, interaction between these strains and their environment has gained increased interest in recent years. This paper presents an overview of the important features of stress responses in Lb. casei, and related proteomic or gene expression patterns that may improve their use as starter cultures and probiotics.

  7. Lactobacillus casei as a biocatalyst for biofuel production.

    Science.gov (United States)

    Vinay-Lara, Elena; Wang, Song; Bai, Lina; Phrommao, Ekkarat; Broadbent, Jeff R; Steele, James L

    2016-09-01

    Microbial fermentation of sugars from plant biomass to alcohols represents an alternative to petroleum-based fuels. The optimal biocatalyst for such fermentations needs to overcome hurdles such as high concentrations of alcohols and toxic compounds. Lactic acid bacteria, especially lactobacilli, have high innate alcohol tolerance and are remarkably adaptive to harsh environments. This study assessed the potential of five Lactobacillus casei strains as biocatalysts for alcohol production. L. casei 12A was selected based upon its innate alcohol tolerance, high transformation efficiency and ability to utilize plant-derived carbohydrates. A 12A derivative engineered to produce ethanol (L. casei E1) was compared to two other bacterial biocatalysts. Maximal growth rate, maximal optical density and ethanol production were determined under conditions similar to those present during alcohol production from lignocellulosic feedstocks. L. casei E1 exhibited higher innate alcohol tolerance, better growth in the presence of corn stover hydrolysate stressors, and resulted in higher ethanol yields. PMID:27312380

  8. Brevibacterium casei isolated as a cause of relapsing peritonitis.

    Science.gov (United States)

    Althaf, Mohammed Mahdi; Abdelsalam, Mohamed Said; Alsunaid, Mohammed Sunaid; Hussein, Maged Hassan

    2014-01-01

    We report a case of relapsing peritonitis in a 33-year-old woman on automated peritoneal dialysis. End-stage renal disease was secondary to systemic lupus erythematosus complicated with lupus nephritis. The organism isolated was Brevibacterium casei that was not readily identified, delaying appropriate management with an extended antibiotic course. Definite management of B casei peritonitis was peritoneal dialysis catheter removal. PMID:24648477

  9. Hazelnut milk fermentation using probiotic Lactobacillus rhamnosus GG and inulin

    OpenAIRE

    Bernat Pérez, Neus; Cháfer Nácher, María Teresa; Chiralt Boix, Mª Amparo; González Martínez, María Consuelo

    2014-01-01

    Following the consumer demand of healthy vegetable products due to their interesting nutritional profiles and potential functionalities, the fermentation process of hazelnut milk with Lactobacillus rhamnosus GG and S.thermophilus was studied. The effect of different factors (glucose, inulin and inoculum contents) was analysed to ensure sufficient probiotic survivals in a minimum time. The shelf life of the optimised product was characterised in terms of its main physicochemical and quality pa...

  10. Stability of Lactobacillus rhamnosus GG in prebiotic edible films

    OpenAIRE

    Soukoulis, Christos; Behboudi-Jobbehdar, Solmaz; Yonekura, Lina; Parmenter, C.D.J.; Fisk, Ian D.

    2014-01-01

    The concept of prebiotic edible films as effective vehicles for encapsulating probiotic living cells is presented. Four soluble fibres (inulin, polydextrose, glucose-oligosaccharides and wheat dextrin) were selected as prebiotic co-components of gelatine based matrices plasticised with glycerol and used for the immobilisation of Lactobacillus rhamnosus GG. The addition of prebiotics was associated with a more compact and uniform film structure, with no detectable interspaces or micropores; pr...

  11. Analysis of the peptidoglycan hydrolase complement of Lactobacillus casei and characterization of the major γ-D-glutamyl-L-lysyl-endopeptidase.

    Directory of Open Access Journals (Sweden)

    Krzysztof Regulski

    Full Text Available Peptidoglycan (PG is the major component of Gram positive bacteria cell wall and is essential for bacterial integrity and shape. Bacteria synthesize PG hydrolases (PGHs which are able to cleave bonds in their own PG and play major roles in PG remodelling required for bacterial growth and division. Our aim was to identify the main PGHs in Lactobacillus casei BL23, a lactic acid bacterium with probiotic properties.The PGH complement was first identified in silico by amino acid sequence similarity searches of the BL23 genome sequence. Thirteen PGHs were detected with different predicted hydrolytic specificities. Transcription of the genes was confirmed by RT-PCR. A proteomic analysis combining the use of SDS-PAGE and LC-MS/MS revealed the main seven PGHs synthesized during growth of L. casei BL23. Among these PGHs, LCABL_02770 (renamed Lc-p75 was identified as the major one. This protein is the homolog of p75 (Msp1 major secreted protein of Lactobacillus rhamnosus GG, which was shown to promote survival and growth of intestinal epithelial cells. We identified its hydrolytic specificity on PG and showed that it is a γ-D-glutamyl-L-lysyl-endopeptidase. It has a marked specificity towards PG tetrapeptide chains versus tripeptide chains and for oligomers rather than monomers. Immunofluorescence experiments demonstrated that Lc-p75 localizes at cell septa in agreement with its role in daughter cell separation. It is also secreted under an active form as detected in zymogram. Comparison of the muropeptide profiles of wild type and Lc-p75-negative mutant revealed a decrease of the amount of disaccharide-dipeptide in the mutant PG in agreement with Lc-p75 activity. As a conclusion, Lc-p75 is the major L. casei BL23 PGH with endopeptidase specificity and a key role in daughter cell separation. Further studies will aim at investigating the role of Lc-p75 in the anti-inflammatory potential of L. casei BL23.

  12. Development of an Escherichia coli-Lactobacillus casei shuttle vector for heterologous protein expression in Lactobacillus casei.

    Science.gov (United States)

    Suebwongsa, Namfon; Lulitanond, Viraphong; Mayo, Baltasar; Yotpanya, Panjamaporn; Panya, Marutpong

    2016-01-01

    There is an increasing interest to develop various lactic acid bacteria (LAB) species as mucosal delivery vehicles, for which the development of a variety of cloning and expression systems for these bacteria is of primary importance. This study reports the complete nucleotide sequence of the cryptic plasmid pRCEID7.6 derived from the chicken probiotic LAB strain Lactobacillus casei TISTR1341. Sequence analysis and comparison showed that pRCEID7.6 is composed of nine putative open reading frames. The replicon origin of pRCEID7.6 consisted of untranslated origin of replication and translated replication protein B sequences. This region was used to construct Escherichia coli/L. casei shuttle vectors carrying erythromycin and chloramphenicol resistance genes as selective markers. Segregation and structural stability of the vectors in L. casei was sufficient for most genetic applications. The feasibility of this vector for heterologous protein expression in L. casei was determined by cloning in pRCEID-LC7.6, the gene encoding the nucleocapsid protein (NP), from the influenza A virus under the control of the homologous promoter from the lactate dehydrogenase gene. L. casei carrying this recombinant plasmid was shown to successfully express the NP protein. Therefore, this shuttle vector can be used for further study in the development of mucosal delivery vehicles. PMID:27026866

  13. Mutagenic effects of microwave irradiation on high yield L-lattic acid producing strain Lactobacillus casei%微波辐照对干酪乳杆菌高产L-乳酸的诱变效应

    Institute of Scientific and Technical Information of China (English)

    徐伟; 王鹏; 张兴

    2007-01-01

    在低功率微波条件下,并采用水循环(20℃)冷却装置,对干酪乳杆菌鼠李糖亚种(Lactobacillus casei subsp.rhamnosus)CICC 6013进行诱变处理,通过对微波辐照功率和微波辐照时间做单因素平行实验,得到最高产酸量为124.15g/L的突变株W4-23,比原始菌株产酸发酵量提高了53.45%,连续遗传8代,产酸性状稳定.研究结果表明,最佳诱变条件为在400W微波功率下辐照20min.

  14. Lactobacillus rhamnosus GG as an Effective Probiotic for Murine Giardiasis

    OpenAIRE

    Nisha Goyal; Ram Prakash Tiwari; Geeta Shukla

    2011-01-01

    The gut microflora is an important constituent in the intestinal mucosal barrier and has been introduced as the concept of probiotic therapy that beneficially affects the host by improving its intestinal microbial balance. Therefore, the main objective of the study was to explore the protective potential of various lactobacilli strains for murine giardiasis. By experimentation, it was found that the probiotic supplementation of either Lactobacillus casei, L. acidophilus, L. plantarum, or L. r...

  15. Whole-genome shotgun sequencing of Lactobacillus rhamnosus MTCC 5462, a strain with probiotic potential.

    Science.gov (United States)

    Prajapati, J B; Khedkar, C D; Chitra, J; Suja, Senan; Mishra, V; Sreeja, V; Patel, R K; Ahir, V B; Bhatt, V D; Sajnani, M R; Jakhesara, S J; Koringa, P G; Joshi, C G

    2012-03-01

    Lactobacillus rhamnosus MTCC 5462 was isolated from infant gastrointestinal flora. The strain exhibited an ability to reduce cholesterol and stimulate immunity. The strain has exhibited positive results in alleviating gastrointestinal discomfort and good potential as a probiotic. We sequenced the whole genome of the strain and compared it to the published genome sequence of Lactobacillus rhamnosus GG (ATCC 53103). PMID:22328760

  16. Characterization of the SpaCBA Pilus Fibers in the Probiotic Lactobacillus rhamnosus GG

    NARCIS (Netherlands)

    Reunanen, J.; Ossowski, von I.; Hendrickx, A.P.; Palva, A.; Vos, de W.M.

    2012-01-01

    Lactobacillus rhamnosus GG is a human intestinal isolate that has been studied intensively because of its probiotic properties. We have previously shown that L. rhamnosus GG produces proteinaceous pili that earlier had been observed only in Gram-positive pathogens (M. Kankainen et al., Proc. Natl. A

  17. The effect of antioxidants on Lactobacillus casei cultures

    Directory of Open Access Journals (Sweden)

    Aleksandra Duda-Chodak

    2008-12-01

    Full Text Available The growing popularity of functional foods causes increasing interest in raw materials, which can raise the pro-health value of food when supplemented. The aim of the study was to assess the effect of compounds with antioxidant properties on Lactobacillus casei, as a representative of probiotic microorganisms. In the experiments the pure antioxidants and plant extracts were used. The results showed that catechin at concentration of 100-400 M and chlorogenic acid (400 M had a stimulatory effect on L. casei growth. Quercetin at concentrations of 25-50 M showed an inhibitory effect when incubation time was  6 h. The lingonberry leaves extract caused a slight increase in the dry substance of biomass in comparison with control. Extracts of coffee, chokeberry, and dog rose should not be used as additives for probiotic food as they significantly inhibited L. casei. We conclude that: 1 antioxidants at concentrations higher than 100 M may stimulate growth of L. casei; 2 except the antioxidants, some other compounds which are present in plants extracts e.g. tannins and alkaloids may exert an antibacterial influence; 3 the probiotic yoghurts supplementation with fruit and other plant materials should be preceded by careful studies about their influence on the bacteria.

  18. In vitro antagonistic activity of Lactobacillus casei against Helicobacter pylori

    Directory of Open Access Journals (Sweden)

    Shymaa Enany

    2015-01-01

    Full Text Available Helicobacter pylori is one of the most common causes of chronic infections in humans. Curing H. pylori infection is difficult because of the habitat of the organism below the mucus adherent layer of gastric mucosa. Lactobacilli are known as acid-resistant bacteria and can remain in stomach for a long time than any other organism, we aimed in this study to examine the efficacy of Lactobacillus casei as a probiotic against H. pylori in humans. Particularly, L. casei was opted as it is considered to be one of the widely used probiotics in dairy products. One hundred and seven strains of H. pylori were isolated from dyspeptic patients and were tested for their antibiotic susceptibility to metronidazole (MTZ, clarithromycin (CLR, tetracycline (TET, and amoxicillin (AMX by the disc diffusion method. The strains were examined for their susceptibility toward L. casei - present in fermented milk products - by well diffusion method. It was found that 74.7% strains were resistant to MTZ; 1.8% to MTZ, TET, and CLR; 3.7% to MTZ and CLR; 4.6% to MTZ and TET; and 0.9% were resistant to MTZ, TET, and AMX. The antibacterial activity of L. casei against H. pylori was determined on all the tested H. pylori isolates including antibiotic resistant strains with different patterns. Our study proposed the use of probiotics for the treatment of H. pylori infection as an effective approach.

  19. In vitro antagonistic activity of Lactobacillus casei against Helicobacter pylori.

    Science.gov (United States)

    Enany, Shymaa; Abdalla, Salah

    2015-01-01

    Helicobacter pylori is one of the most common causes of chronic infections in humans. Curing H. pylori infection is difficult because of the habitat of the organism below the mucus adherent layer of gastric mucosa. Lactobacilli are known as acid-resistant bacteria and can remain in stomach for a long time than any other organism, we aimed in this study to examine the efficacy of Lactobacillus casei as a probiotic against H. pylori in humans. Particularly, L. casei was opted as it is considered to be one of the widely used probiotics in dairy products. One hundred and seven strains of H. pylori were isolated from dyspeptic patients and were tested for their antibiotic susceptibility to metronidazole (MTZ), clarithromycin (CLR), tetracycline (TET), and amoxicillin (AMX) by the disc diffusion method. The strains were examined for their susceptibility toward L. casei - present in fermented milk products - by well diffusion method. It was found that 74.7% strains were resistant to MTZ; 1.8% to MTZ, TET, and CLR; 3.7% to MTZ and CLR; 4.6% to MTZ and TET; and 0.9% were resistant to MTZ, TET, and AMX. The antibacterial activity of L. casei against H. pylori was determined on all the tested H. pylori isolates including antibiotic resistant strains with different patterns. Our study proposed the use of probiotics for the treatment of H. pylori infection as an effective approach.

  20. Effect of Lactobacillus casei- casei and Lactobacillus reuteri on acrylamide formation in flat bread and Bread roll.

    Science.gov (United States)

    Dastmalchi, Farnaz; Razavi, Seyed Hadi; Faraji, Mohammad; Labbafi, Mohsen

    2016-03-01

    The aim of this study was the evaluation of fermentation by lactic acid bacteria (LAB) contains lactobacillus (L.) casei- casei and L. reuteri on acrylamide formation and physicochemical properties of the Iranian flat bread named, Sangak, and Bread roll. Sangak and Bread roll were made with whole and white wheat flour, respectively. Whole-wheat flour had upper content of protein, sugar, ash, fiber, damaged starch and the activity of amylase than the white wheat flour. After 24 h of fermentation, the pH values of the sourdoughs made from whole-wheat flour (3.00, 2.90) were lower, in compared to sourdoughs prepared from white wheat flour (3.60, 3.58). In addition, in Sangak bread, glucose, and fructose were completely utilized after fermentation, but in bread roll, the reduced sugar levels increased after fermentation and baking that represent microorganisms cannot be activated and utilized sugars. Acrylamide formation was impacted by pH of sourdough and total reducing sugar (r = 0.915, r = 0.885 respectively). Bread roll and Sangak bread were fermented by L. casei- casei contained lowest acrylamide content, in two bread types (219.1, 104.3 μg/kg respectively). As an important result, the acrylamide content of Sangak bread in all cases was lower than in the Bread roll. PMID:27570278

  1. The development-specific ssp1 and ssp2 genes of Sclerotinia sclerotiorum encode lectins with distinct yet compensatory regulation.

    Science.gov (United States)

    Li, Moyi; Rollins, Jeffrey A

    2010-06-01

    The Ssp1 development-specific protein is the most abundant soluble protein in sclerotia and apothecia of Sclerotinia sclerotiorum. Although closely associated with these developmental stages, the functions of the Ssp1 protein and its paralog, Ssp2, are not known. In this study, protein structure prediction analysis revealed that Ssp1 and Ssp2 are structurally similar to fucose-specific lectins. In an effort to understand the function of these abundant, development-specific proteins, a homokaryotic ssp1 deletion mutant was generated. The resulting mutant (Deltassp1) displays a wild-type growth and development phenotype in culture but produces approximately 50% fewer sclerotia in cultures supplemented with hygromycin. Genetic complementation with a wild-type copy of ssp1 restores normal sclerotium formation in the presence of hygromycin. This suggests that Ssp1 might play a role in resistance to glycoside-containing antibiotics encountered in the environment. Although a slight delay in carpogenic germination was observed, no additional effects of ssp1 loss-of-function were found in regards to apothecial morphology or fecundity. When the expression of ssp2 was examined in the Deltassp1 mutant, it was found to be expressed earlier in sclerotial development and its encoded protein accumulated to higher levels in both sclerotia and apothecia. These findings suggest regulatory compensation for loss of Ssp1 coupled with potential functional redundancy among lectins accumulating in sclerotia and apothecia. PMID:20350614

  2. The effect of antioxidants on Lactobacillus casei cultures

    OpenAIRE

    Aleksandra Duda-Chodak; Tomasz Tarko; Mateusz Statek

    2008-01-01

    The growing popularity of functional foods causes increasing interest in raw materials, which can raise the pro-health value of food when supplemented. The aim of the study was to assess the effect of compounds with antioxidant properties on Lactobacillus casei, as a representative of probiotic microorganisms. In the experiments the pure antioxidants and plant extracts were used. The results showed that catechin at concentration of 100-400 M and chlorogenic acid (400 M...

  3. Adhesion properties of Lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins.

    Science.gov (United States)

    Nishiyama, Keita; Nakamata, Koichi; Ueno, Shintaro; Terao, Akari; Aryantini, Ni Putu Desy; Sujaya, I Nengah; Fukuda, Kenji; Urashima, Tadasu; Yamamoto, Yuji; Mukai, Takao

    2015-01-01

    We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wild-type strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.

  4. Suitability of cereal porridges as substrate for probiotic strain Lactobacillus rhamnosus GG

    OpenAIRE

    Ľubomír Valík; Monika Kocková

    2013-01-01

    The aim of this work was to find new substrates suitable for growth and metabolism of probiotic strain Lactobacillus rhamnosus GG, which would be interesting for development of new functional food. The growth and metabolic activity of Lb. rhamnosus GG in cereal (rye, barley, oat and millet) porridges were monitored during fermentation process. Cereal and porridges, were inoculated with this strain at two initial levels to obtain approximately 5 or 6 log colony form units per gram of suspensio...

  5. Oral ecology and virulence of Lactobacillus casei and Streptococcus mutans in gnotobiotic rats.

    Science.gov (United States)

    Michalek, S M; Hirasawa, M; Kiyono, H; Ochiai, K; McGhee, J R

    1981-01-01

    Lactobacilli comprise a small percentage of the normal oral microbial flora of humans and are isolated commonly from saliva and frequently from an active caries lesion. We have compared the pathogenesis and colonization pattern of Lactobacillus casei with that of Streptococcus mutans strain 6715 in gnotobiotic rats. Of the two L. casei strains tested, L. casei strain ATCC 4646 caused slightly more caries than L. casei strain ATCC 11578. However, the level of caries induced by either L. casei strain was significantly lower (P less than 0.01) than that observed in similar-aged rats monoassociated with S. mutans strain 6715. When groups of rats were infected with mixtures of L. casei strain ATCC 4646 and S. mutans strain 6715, or with L. casei followed by S. mutans, higher numbers of L. casei than S. mutans were found associated with the tongue and in saliva; S. mutans always predominated in plaque. The level of caries observed in these groups of rats was similar to that seen with rats monoassociated with S. mutans except when L. casei comprised greater than 1% of the plaque microflora. In this latter situation, the level of caries was significantly lower (P less than or equal to 0.05) than that obtained in S. mutans-monoassociated rats. The results of this study suggest that L. casei colonizes sites in the oral cavity (including the tongue and saliva) other than the tooth surface in rats. The effect of L. casei in plaque toward reduction of S. mutans-induced dental caries in rats is discussed. PMID:6793515

  6. Brevibacterium casei Sepsis in an 18-Year-Old Female with AIDS

    OpenAIRE

    Brazzola, P; Zbinden, R; Rudin, C.; Schaad, U B; HEININGER, U.

    2000-01-01

    Brevibacterium sp. was isolated from the blood of an acutely ill 18-year-old female with AIDS. The isolate was identified as Brevibacterium casei by use of carbohydrate assimilation tests. Treatment was successful with intravenously administered ciprofloxacin. To our knowledge, this is the first report of sepsis caused by B. casei in a human immunodeficiency virus-infected patient.

  7. Brevibacterium casei as a Cause of Brain Abscess in an Immunocompetent Patient ▿

    OpenAIRE

    Kumar, V. Anil; Augustine, Deepthi; Panikar, Dilip; Nandakumar, Aswathy; Kavitha R Dinesh; Karim, Shamsul; Philip, Rosamma

    2011-01-01

    Coryneform bacteria belonging to the genus Brevibacterium have emerged as opportunistic pathogens. Of the nine known species of Brevibacterium isolated from human clinical samples, Brevibacterium casei is the most frequently reported species from clinical specimens. We report the first case of B. casei brain abscess in an immunocompetent patient successfully treated by surgery and antimicrobial therapy.

  8. Investigation of Biomarkers of Bile Tolerance in Lactobacillus casei Using Comparative Proteomics

    NARCIS (Netherlands)

    Hamon, Erwann; Horvatovich, Peter; Bisch, Magali; Bringel, Francoise; Marchioni, Eric; Aoude-Werner, Dalal; Ennahar, Said

    2012-01-01

    The identification of cell determinants involved in probiotic features is a challenge in current probiotic research. In this work, markers of bile tolerance in Lactobacillus casei were investigated using comparative proteomics. Six L. casei strains were classified on the basis of their ability to gr

  9. Draft Genome Sequence of the Respiration-Competent Strain Lactobacillus casei N87.

    Science.gov (United States)

    Zotta, Teresa; Ricciardi, Annamaria; Parente, Eugenio; Reale, Anna; Ianniello, Rocco G; Bassi, Daniela

    2016-01-01

    Lactobacillus casei is used as a starter, adjunct, and/or probiotic culture in the production of fermented and functional foods. Here, we report the draft genome sequence of the respiration-competent strain L. casei N87, isolated from infant feces. This genome information may be useful for the study of respiratory metabolism in lactic acid bacteria. PMID:27151805

  10. Lactobacillus paracasei subsp paracasei L. casei W8 suppresses energy intake acutely

    DEFF Research Database (Denmark)

    Bjerg, Anne Toksvig; Kristensen, Mette Bredal; Ritz, Christian;

    2014-01-01

    Background: Probiotic bacteria have been shown to have various effects on the microbiota; this may also affect appetite and may help promote weight loss and maintenance. Objective: This study was conducted to investigate the effect of Lactobacillus paracasei subsp paracasei L. casei W8 (L. casei W8...

  11. [Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens].

    Science.gov (United States)

    Lin, Hongli; Hou, Shenda; Wang, Song; Wang, Yupeng; LuanI, Yunyan; Hou, Xilin

    2014-11-01

    In order to determine immunogenicity and protective effect in chickens, we used the IBDV (Infectious bursal disease virus)-Vp2/Lactobacillus casei as antigen transfer system. First, the immunized and control chickens were challenged by IBDV/DQ at lethal dose to determine the protective ratio. Second, chickens were orallyand intranasally vaccinated twice with 10(9) CFU/mL pLA-VP2/L. casei, pLA/L. casei and PBS as negativecontrol and commercial vaccine as positive control. The bursa injury and the lesion score wererecorded post challenge. The level of specific IgG and sIgA in pLA-VP2/L. casei and positive control groups was significantly higher than that in negativecontrol groups. The protection efficacy in pLA-VP2/L. casei oral group was higher than that inintranasal group. The SI. of pLA-VP2/L. casei oral group was significant higher than other groups. The lesion score indicated the pLA-VP2/L. casei was safer than commercial vaccine for bursa. Collectively, the pLA-VP2/L. casei could be a vaccine candidate for IBDV. PMID:25985519

  12. 干酪乳杆菌Lactobacillus casei 393 upp基因突变株的构建%Construction of an Lactobacillus Casei 393 upp mutant

    Institute of Scientific and Technical Information of China (English)

    郭建军; 袁林; 曾静; 付锦楠; 龚小华; 何顺华

    2015-01-01

    upp基因可用来作为反向筛选标记基因.利用pORI温度敏感型双质粒系统敲除干酪乳杆菌Lactobacillus casei 393的upp基因,构建得到重组菌Lactobacillus casei 393-△upp.重组菌可作为反向筛选标记基因upp使用的宿主菌.

  13. Enhancement of host resistance against Listeria infection by Lactobacillus casei: Role of macrophages

    International Nuclear Information System (INIS)

    Among the 10 species of the genus Lactobacillus, L. casei showed the strongest protective action against Listeria monocytogenes infection in mice. The activity of L. casei differed with regard to the dose of administration. The anti-L. monocytogenes resistance in mice intravenously administered 5.5 X 10(7), 2.8 X 10(8), or 1.1 X 10(9) L. casei cells was most manifest at ca. 2, 2 and 13, and 3 to 21 days after its administration, respectively. The growth of L. monocytogenes in the liver of mice injected with L. casei (10(7), 10(8), or 10(9) cells) 48 h after infection was suppressed, particularly when 10(8) or 10(9) L. casei cells were given 2 or 13 days before the induced infection, respectively. This suppression of L. monocytogenes growth was overcome by carrageenan treatment or X-ray irradiation. [3H]thymidine incorporation into the liver DNA increased 13 days after administration of L. casei, and augmentation of [3H]thymidine incorporation during 6 to 48 h after infection was dependent on the dose of L. casei. Peritoneal macrophage accumulation observed 1 to 5 days after intraperitoneal injection of UV-killed L. monocytogenes was markedly enhanced when the mice were treated with L. casei cells 13 days before macrophage elicitation. Therefore, the enhanced host resistance by L. casei to L. monocytogenes infection may be mediated by macrophages migrating from the blood stream to the reticuloendothelial system in response to L. casei injection before or after L. monocytogenes infection

  14. Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus

    Directory of Open Access Journals (Sweden)

    Alli SMA

    2011-08-01

    Full Text Available Sk Md Athar Alli Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaBackground: The purpose of this study was to develop a mucoadhesive coacervate microparticulate system to deliver viable Lactobacillus rhamnosus cells into the gut for an extended period of time while maintaining high numbers of viable cells within the formulation throughout its shelf-life and during gastrointestinal transit.Methods: Core coacervate mucoadhesive microparticles of L. rhamnosus were developed using several grades of hypromellose and were subsequently enteric-coated with hypromellose phthalate. Microparticles were evaluated for percent yield, entrapment efficiency, surface morphology, particle size, size distribution, zeta potential, flow properties, in vitro swelling, mucoadhesion properties, in vitro release profile and release kinetics, in vivo probiotic activity, and stability. The values for the kinetic constant and release exponent of model-dependent approaches, the difference factor, similarity factor, and Rescigno indices of model-independent approaches were determined for analyzing in vitro dissolution profiles.Results: Experimental microparticles of formulation batches were of spherical shape with percent yields of 41.24%–58.18%, entrapment efficiency 45.18%–64.16%, mean particle size 33.10–49.62 µm, and zeta potential around -11.5 mV, confirming adequate stability of L. rhamnosus at room temperature. The in vitro L. rhamnosus release profile follows zero-order kinetics and depends on the grade of hypromellose and the L. rhamnosus to hypromellose ratio.Conclusion: Microparticles delivered L. rhamnosus in simulated intestinal conditions for an extended period, following zero-order kinetics, and exhibited appreciable mucoadhesion in simulated intestinal conditions.Keywords: Lactobacillus rhamnosus, mucoadhesive, microparticles, extended-release, intestine

  15. 利用Lactobacillus casei Zhang开发益生菌新鲜干酪%Development a New Probiotic Fresh Cheese With Lactobacillus casei Zhang

    Institute of Scientific and Technical Information of China (English)

    董成; 张和平; 赵树平; 包秋华

    2008-01-01

    通过添加不同比例Lactobacillus casei Zhang发酵剂制作新鲜干酪,并对其在新鲜干酪中的活力和添加Lactobacillus后新鲜干酪的理化性质进行了研究.结果表明,Lb.casei Zhang在新鲜干酪中具有较高的活力,添加2%、1%、0.5% Lb.casei Zhang发酵剂的干酪中,4℃冷藏开始前,Lb.casei Zhang活菌数分别为2.24×108cfu/g,1.38×108cfu/g,5.55×107cfu/g,4℃冷藏28d后,Lb.casei Zhang存活率分别为99.12%,98.31%,98.61%.在制作过程中和4℃冷藏过程中,与空白组相比,添加Lb.casei Zhang对新鲜干酪的pH值、滴定酸度、蛋白水解活性影响都不显著(P>0.05).

  16. 微波辐照选育L-乳酸高产菌的生物学特性研究%Study on screening high yield L-actic acid producing strain Lactobacillus casei by microwave mutagenesis and biological characters of mutant strain

    Institute of Scientific and Technical Information of China (English)

    张兴; 王鹏; 徐伟

    2008-01-01

    在低功率微波条件下,采用水循环冷却器,对干酪乳杆菌鼠李糖亚种(Lactobacillus casei subsp. rhamnosus)X1-12进行诱变处理,在微波功率400 W,辐照时间3 min的诱变条件下得到L-乳酸产量为115.8 g/L的突变株W3-9,比原始菌株X1-12提高了57.98%,连续遗传8代,产酸性状稳定.通过原始菌株和突变株的生理生化实验,可知微波辐照后的菌株生物学特性发生部分改变.

  17. The Huygens Surface Science Package (SSP)

    Science.gov (United States)

    Zarnecki, John C.; Leese, Mark; Hathi, Brijen; SSP Consortium, The

    2015-04-01

    The Huygens Surface Science Package (SSP), one of the six instruments selected by ESA, was the only one whose prime purpose was to study the surface of Titan in-situ. Designed at a time when even the basic nature of the surface (i.e. solid ice or liquid hydrocarbons) was unknown, it consisted of 9 separate compact and relatively simple sensors designed to span all likely surface scenarios. The Huygens landing on a "dry lake bed" allowed some of the sensors to be fully exercised. The results and their subsequent interpretations will be presented as well as perspectives from nearly 25 years since the original instrument selection.

  18. Abietatrienes diterpenoids from Sagittaria montevidensis SSP Montevidensis

    Energy Technology Data Exchange (ETDEWEB)

    Tanaka, Clara M.A.; Radke, Vanessa S.C.O.; Silva, Cleuza C. da [Universidade Estadual de Maringa (UEM), PR (Brazil). Dept. de Quimica; Nakamura, Celso V. [Universidade Estadual de Maringa (UEM), PR (Brazil). Dept. de Analises Clinicas; Oliveira, Pollyanna L. de; Kato, Lucilia; Oliveira, Cecilia M.A. de [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Inst. de Quimica

    2010-07-01

    The antimicrobial properties of the hexane, hexane/EtOAc and methanol fractions of the fresh petioles of Sagittaria montevidensis ssp montevidensis (Alismataceae) were evaluated against fungi and Gram-negative and Gram-positive bacteria. A new abietatriene-type diterpenoid, 3beta,7alpha-dihydroxi-abieta-8,11,13-triene and the known 3beta-hydroxy-abieta-8,11,13-trien-7-one were isolated from the most active fraction tested and the structures of these compounds were elucidated by data including IR, EIMS, and 1D and 2D NMR spectra. (author)

  19. Development of an alternative culture medium for the selective enumeration of Lactobacillus casei in fermented milk.

    Science.gov (United States)

    Colombo, Monique; de Oliveira, Aline Evelyn Zimmermann; de Carvalho, Antonio Fernandes; Nero, Luís Augusto

    2014-05-01

    Monitoring the populations of probiotic strains of the species Lactobacillus casei in food is required by food industries in order to assure that a minimum concentration of these organisms will be ingested by consumers. In this context, Petrifilm™ AC plates can be used along with selective culture media to allow the enumeration of specific groups of lactic acid bacteria. The present study aimed to assess chemical substances as selective agents for Lb. casei in order to propose a selective culture medium to be used with Petrifilm™ AC plates as an alternative protocol for the enumeration of probiotic strains of this species in fermented milk. Twenty-six probiotic and starter cultures (including six strains of Lb. casei) were plated on de Man Rogosa and Sharpe (MRS) agar with distinct concentrations of nalidixic acid, bile, lithium chloride, metronidazole, sodium propionate, and vancomycin. Vancomycin at 10 mg/L demonstrated selective activity for Lb. casei. In addition, 2,3,5-triphenyltetrazolium chlorine was identified as a compound that did not inhibit Lb. casei, and Petrifilm™ AC plates used with MRS and vancomycin at 10 mg/L (MRS-V) demonstrated more colonies of this organism when incubated under anaerobic conditions than aerobic conditions. Acidophilus milk and yoghurt were prepared, added to Lb. casei strains, and stored at 4 °C. Lb. casei populations were monitored using MRS-V and MRTLV by conventional plating and associated with Petrifilm™ AC plates. All correlation indices between counts obtained by conventional plating and Petrifilm™ AC were significant (p enumeration of Lb. casei strains in fermented milk. However, the selective potential of this culture medium must be evaluated considering the specific strains of Lb. casei and the starter cultures inoculated in the fermented milk that requires monitoring.

  20. Examination of the technological properties of newly isolated strains of the genus Lactobacillus and possibilities for their application in the composition of starters

    OpenAIRE

    Denkova, Rositsa; Ilieva, Svetla; Denkova, Zapryana; Georgieva, Ljubka; Krastanov, Albert

    2014-01-01

    The ability of four Lactobacillus strains – Lactobacillus brevis LBRZ7 (isolated from fermented cabbage), Lactobacillus plantarum LBRZ12 (isolated from fermented cabbage), Lactobacillus fermentum LBRH9 (of human origin) and Lactobacillus casei ssp. rhamnosus LBRC11 (isolated from home-made cheese) – to grow in flour/water environment and to accumulate high concentrations of viable cells was examined. Two starters for sourdough were created for lab-scale production of wheat bread: a two-strain...

  1. Risks Associated with High-Dose Lactobacillus rhamnosus in an Escherichia coli Model of Piglet Diarrhoea

    DEFF Research Database (Denmark)

    Li, Xiao-Qiong; Zhu, Yao-Hong; Zhang, Hong-Fu;

    2012-01-01

    Probiotic could be a promising alternative to antibiotics for the prevention of enteric infections; however, further information on the dose effects is required. In this study, weanling piglets were orally administered low- or high-dose Lactobacillus rhamnosus ACTT 7469 (1010 CFU/d or 1012 CFU...... by reduced coliform shedding in faeces. Interestingly, L. rhamnosus administration reduced Lactobacillus and Bifidobacterium counts in the colonic contents, and the high-dose piglets also had lower Lactobacillius and Bacteroides counts in the ileal contents. An increase in the concentration of serum TNF......-α induced by F4+ETEC was observed, but the increase was delayed by L. rhamnosus. In piglets exposed to F4+ETEC, jejunal TLR4 expression increased at the mRNA and protein levels, while jejunal interleukin (IL)-8 and ileal porcine β-defensins 2 (pBD2) mRNA expression increased; however, these increases were...

  2. Submerged fermentation of Lactobacillus rhamnosus YS9 for γ-aminobutyric acid (GABA production

    Directory of Open Access Journals (Sweden)

    Qian Lin

    2013-01-01

    Full Text Available γ-Aminobutyric acid (GABA is a major inhibitory neurotransmitter in central nervous system, and its application in drugs and functional foods has attracted great attention. To enhance production of y-aminobutyric acid, Lactobacillus rhamnosus YS9, a strain isolated from Chinese traditional fermented food pickled vegetable, was grown under submerged fermentation. Its cultivation conditions were investigated. When culture pH condition was adjusted to the optimal pH of glutamate decarboxylase activity, culture of Lb. rhamnosus YS9 in medium supplemented with 200 mM of monosodium glutamate and 200 µM of pyridoxal phosphate (PLP, produced 187 mM of GABA.

  3. Complete genome sequence of the probiotic Lactobacillus casei strain BL23.

    NARCIS (Netherlands)

    Maze, A.; Boel, G.; Zuniga, M.; Bourand, A.; Loux, V.; Yebra, M.J.; Monedero, V.; Correia, K.; Jacques, N.; Beaufils, S.; Poncet, S.; Joyet, P.; Milohanic, E.; Casaregola, S.; Auffray, Y.; Perez-Martinez, G.; Gibrat, J.F.; Zagorec, M.; Francke, C.; Hartke, A.; Deutscher, J.

    2010-01-01

    The entire genome of Lactobacillus casei BL23, a strain with probiotic properties, has been sequenced. The genomes of BL23 and the industrially used probiotic strain Shirota YIT 9029 (Yakult) seem to be very similar.

  4. Post-traumatic endophthalmitis due to Brevibacterium casei : A case report

    OpenAIRE

    Asima Banu; Sriprakash KS; Vidyadevi M; ER, Nagraj

    2013-01-01

    Endophthalmitis is a serious post-traumatic ocular complication that can lead to loss of vision. We report a case of acute post-traumatic endophthalmitis following a penetrating injury caused by an unusual organism, Brevibacterium casei . The patient was successfully treated with intravitreal antibiotics like ceftazidime and vancomycin, along with topical cefazolin and tobramycin. Brevibacterium casei can be added to the list of rare bacteria causing endophthalmitis and should be kept in mind...

  5. Human infections caused by Brevibacterium casei, formerly CDC groups B-1 and B-3.

    OpenAIRE

    Gruner, E; Steigerwalt, A G; Hollis, D G; Weyant, R S; Weaver, R E; Moss, C W; M Daneshvar; J. M. Brown; Brenner, D J

    1994-01-01

    Forty-one clinical strains of CDC coryneform groups B-1 and B-3 were compared biochemically, by analysis of cell wall sugars, amino acids, and cellular fatty acids, and by DNA relatedness to the type strains of Brevibacterium casei, Brevibacterium epidermidis, and Brevibacterium linens. Twenty-two strains were shown to be B. casei, while five other strains formed a phenotypically inseparable genomospecies in the same genus. The remaining isolates were genetically heterogeneous, and most are p...

  6. KARAKTERISTIK DADIH PROBIOTIK MENGGUNAKAN KOMBINASI LACTOBACILLUS CASEI, LACTOBACILLUS PLANTARUM, DAN BIFIDOBACTERIUM LONGUM SELAMA PENYIMPANAN

    Directory of Open Access Journals (Sweden)

    Sri Usmiati

    2012-03-01

    Full Text Available 800x600 Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 Some test results hedonic by ordinary people who consume the dadih in West Sumatra in 2009 on dadih made from cow's milk using Lactobacillus casei culture couldn’t be accepted because it was still too acidic than dadih from buffalo milk. One solution to reduce the sour taste is to combine the bacterial culture L. casei with other lactic acid bacteria that produce flavor relatively low acidity. The study aimed to determine the characteristics of probiotic dadih using a combination starter L. casei, L. plantarum and B. longum during storage at room temperature (ambient and cold temperature. The study was designed using randomized block design with 6x3 factorial patterns of three groups as replication. Treatment A (combination of probiotic bacteria that L. casei (A1, B. longum (A2, L. plantarum (A3, L. casei: L. plantarum 1:5 (A4, L. casei: B. longum 1:5 (A5 and L. casei: L. plantarum: B. longum 1:4:1 (A6, and factor B (storage conditions, namely: (B1 room temperature (27oC, and (B2 cold temperature (4-10oC. The results showed that viability and the total lactic acid bacteria in all formulas of cow's milk dadih during storage at room temperature and cold temperature of more than 106 cfu/ml which could be categorized as a probiotic products. The combination of C1L5 (L.casei: B.longum 1: 5 has the lowest acidity value and excellence in character color, flavor, and was generally preferred by the panelists. In terms of flavor and texture characteristics of cow's milk dadih with a combination of C1L5 had a level of hedonic as with other formulas.       Keywords: dadih, cows milk, probiotic, storage

  7. KARAKTERISTIK DADIH PROBIOTIK MENGGUNAKAN KOMBINASI LACTOBACILLUS CASEI, LACTOBACILLUS PLANTARUM, DAN BIFIDOBACTERIUM LONGUM SELAMA PENYIMPANAN

    OpenAIRE

    Sri Usmiati; . Juniawati

    2012-01-01

    Some test results hedonic by ordinary people who consume the dadih in West Sumatra in 2009 on dadih made from cow's milk using Lactobacillus casei culture couldn’t be accepted because it was still too acidic than dadih from buffalo milk. One solution to reduce the sour taste is to combine the bacterial culture L. casei with other lactic acid bacteria that produce flavor relatively low acidity. The study aimed to determine the characteristics of probiotic dadih using a combination starter L....

  8. Production of L(+ lactic acid using Lactobacillus casei from whey

    Directory of Open Access Journals (Sweden)

    Parmjit S. Panesar

    2010-02-01

    Full Text Available The aim of this work was to study the fermentation of whey for the production of L(+ lactic acid using Lactobacillus casei. The effect of different process parameters such as pH of the medium, temperature, inoculum size, age of inoculum, agitation and incubation time was monitored to enhance the lactose conversion in whey to L(+ lactic acid. Fermentations were performed without any pH control. The optimization of the fermentation conditions resulted in significant decrease in fermentation time, besides increase in lactose conversion to lactic acid. The optimized process conditions resulted in high lactose conversion (95.62% to L(+ lactic acid production (33.73 g/L after an incubation period of 36 h.

  9. ANTIBACTERIAL ACTIVITY OF Lactobacillus casei IMV B-7280 IN CASES OF EXPERIMENTAL UROGENITAL STAPHYLOCOCCAL INFE

    Directory of Open Access Journals (Sweden)

    Babenko L. P.

    2015-08-01

    Full Text Available The aim of work was to determine antibacterial activity of Lactobacillus casei ІМV В-7280 probiotic strain on the experimental urogenital tract infection of mice. The influence of intravaginal and/or per os administration of this strain once per day during 7 days on the microflora of vagina, kidneys and intestinal contents of Staphylococcus aureus 8325-4 infected mice was studied. It was established, that in cases of experimental staphylococcal infection of urogenital tract L. Casei IMV B-7280 had effective antagonistic activity against S. aureus 8325-4 and opportunistic bacteria. After L. casei IMV B-7280 introduction into infected mice reduction or complete elimination of S. aureus 8325-4 in vagina, kidneys and intestinal contents in different periods of observation was established. Under the influence of L. casei ІМВ В-7280 the number of coliform bacteria, streptococci and staphylococci in the vagina was normalized, and fungal flora — decreased even in comparison with intact mice. Normalization of kidneys microflora was also observed. In the intestinal contents of infected mice trea ted with L. casei IMV B-7280 the number of streptococci did not change, staphylococci number decreased, but the number of fungal and coliform flora remained relatively low during the observation period. L. casei IMV B-7280 probiotic strain is promising to create immunobiotics with antibacterial action, which can be used for the prevention and treatment of urogenital infections caused by opportunistic microorganisms.

  10. Development of probiotic mutandabota, a locally sustainable functional food incorporating Lactobacillus rhamnosus

    NARCIS (Netherlands)

    Mpofu, A.

    2015-01-01

    Development of probiotic mutandabota, a locally sustainable functional food incorporating Lactobacillus rhamnosus Mutandabota or umlondo is an indigenous food that is consumed in Southern Africa on a daily basis. The product is made by mixing raw cow’s or goat’s milk wit

  11. Effect of acid stress on protein expression and phosphorylation in Lactobacillus rhamnosus GG

    NARCIS (Netherlands)

    Koponen, J.; Laakso, K.; Koskenniemi, K.; Kankainen, M.; Savijoki, K.; Nyman, T.A.; Vos, de W.M.; Tynkkynen, S.; Kalkkinen, N.; Varmanen, P.

    2012-01-01

    Acidic environments encountered in food products and during gastrointestinal tract passage affect the survival of bacteria that are marketed as probiotics. In this study, the global proteome responses of the probiotic lactic acid bacterium Lactobacillus rhamnosus GG to two physiologically relevant p

  12. Preserving viability of Lactobacillus rhamnosus GG in vitro and in vivo by a new encapsulation system

    Science.gov (United States)

    Probiotics have shown beneficial effects on human health. To increase the efficacy of probiotic applications, we used Lactobacillus rhamnosus GG (LGG) as a probiotic model to investigate approaches to enhance the bioavailability of probiotics. LGG was encapsulated in hydrogel beads containing pectin...

  13. Genetic expression profile analysis of the temporal inhibition of quercetin and naringenin on Lactobacillus rhamnosus GG

    Science.gov (United States)

    The plant polyphenols, quercetin and naringenin, are considered healthy dietary compounds; however, little is known of their effects on the probiotic Lactobacillus rhamnosus GG (LGG). In this study, it was discovered that both quercetin and naringenin produced temporary inhibition of LGG growth, par...

  14. Colon-specific delivery of lactobacillus rhamnosus GG using pectin hydrogel beads

    Science.gov (United States)

    The probiotic bacteria, Lactobacillus rhamnosus GG (LGG), has shown beneficial effects on human health, and is accepted by increasing populations for the prevention and treatment of irritable bowel diseases. To increase the bioavailability and efficacy of LGG, the probiotic was encapsulated in hydro...

  15. Complete Genome Sequence of the Probiotic Lactic Acid Bacterium Lactobacillus Rhamnosus

    Directory of Open Access Journals (Sweden)

    Samat Kozhakhmetov

    2014-01-01

    Full Text Available Introduction: Lactobacilli are a bacteria commonly found in the gastrointestinal tract. Some species of this genus have probiotic properties. The most common of these is Lactobacillus rhamnosus, a microoganism, generally regarded as safe (GRAS. It is also a homofermentative L-(+-lactic acid producer. The genus Lactobacillus is characterized by an extraordinary degree of the phenotypic and genotypic diversity. However, the studies of the genus were conducted mostly with the unequally distributed, non-random choice of species for sequencing; thus, there is only one representative genome from the Lactobacillus rhamnosus clade available to date. The aim of this study was to characterize the genome sequencing of selected strains of Lactobacilli. Methods: 109 samples were isolated from national domestic dairy products in the laboratory of Center for life sciences. After screaning isolates for probiotic properties, a highly active Lactobacillus spp strain was chosen. Genomic DNA was extracted according to the manufacturing protocol (Wizard® Genomic DNA Purification Kit. The Lactobacillus rhamnosus strain was identified as the highly active Lactobacillus strain accoridng to its morphological, cultural, physiological, and biochemical properties, and a genotypic analysis. Results: The genome of Lactobacillus rhamnosus was sequenced using the Roche 454 GS FLX (454 GS FLX platforms. The initial draft assembly was prepared from 14 large contigs (20 all contigs by the Newbler gsAssembler 2.3 (454 Life Sciences, Branford, CT. Conclusion: A full genome-sequencing of selected strains of lactic acid bacteria was made during the study.

  16. School Teams up for SSP Functional Models

    Science.gov (United States)

    Pignolet, G.; Lallemand, R.; Celeste, A.; von Muldau, H.

    2002-01-01

    Space Solar Power systems appear increasingly as one of the major solutions to the upcoming global energy crisis, by collecting solar energy in space where this is most easy, and sending it by microwave beam to the surface of the planet, where the need for controlled energy is located. While fully operational systems are still decades away, the need for major development efforts is with us now. Yet, for many decision-makers and for most of the public, SSP often still sounds like science fiction. Six functional demonstration systems, based on the Japanese SPS-2000 concept, have been built as a result of a cooperation between France and Japan, and they are currently used extensively, in Japan, in Europe and in North America, for executive presentations as well as for public exhibitions. There is demand for more models, both for science museums and for use by energy dedicated groups, and a senior high school in La Reunion, France, has picked up the challenge to make the production of such models an integrated practical school project for pre-college students. In December 2001, the administration and the teachers of the school have evaluated the feasibility of the project and eventually taken the go decision for the school year 2002- 2003, when for education purposes a temporary "school business company" will be incorporated with the goal to study and manufacture a limited series of professional quality SSP demonstration models, and to sell them world- wide to institutions and advocacy groups concerned with energy problems and with the environment. The different sections of the school will act as the different services of an integrated business : based on the current existing models, the electronic section will redesign the energy management system and the microwave projector module, while the mechanical section of the school will adapt and re-conceive the whole packaging of the demonstrator. The French and foreign language sections will write up a technical manual for

  17. Etude chimique et biologique des huiles essentielles de Juniperus phoenicea ssp. lycia et Juniperus phoenicea ssp. turbinata du Maroc

    Directory of Open Access Journals (Sweden)

    Mansouri, N.

    2011-01-01

    Full Text Available Chemical and biological study of essential oils of Moroccan Juniperus phoenicea ssp. lycia and Juniperus phoenicea ssp. turbinata. The composition of the essential oils of the branches and berries of Juniperus phoenicea (Cupressaceae, J. phoenicea ssp. lycia (plain and J. phoenicea ssp. turbinata (mountain, obtained by hydrodistillation, collected from Morocco, was analyzed by GC and GC/MS. The yields of essential oils were varying in function of the subspecies and of the part of the plant studied. The essential oils of these tree species are largely dominated by α-pinene and may be an important source of this component of a great interest on the international market. The effectiveness of essential oils from branches of the subspecies lycia against fungi decay wood has also been emphasized.

  18. Identification of Independent Streptococcus gordonii SspA and SspB Functions in Coaggregation with Actinomyces naeslundii

    OpenAIRE

    Egland, Paul G.; Dû, Laurence D.; Kolenbrander, Paul E.

    2001-01-01

    The initial stages of dental plaque formation involve the adherence of early colonizing organisms such as Streptococcus gordonii and Actinomyces naeslundii to the saliva-coated tooth surface and to each other. The S. gordonii surface proteins SspA and SspB are known to play a role in adherence to salivary proteins and mediate coaggregation with other bacteria. Coaggregation is the adhesin receptor-mediated interaction between genetically distinct cell types and appears to be ubiquitous among ...

  19. Chemical and Antibacterial Polymorphism of Juniperus oxycedrus ssp. oxycedrus and Juniperus oxycedrus ssp. macrocarpa (Cupressaceae) Leaf Essential Oils from Tunisia

    OpenAIRE

    Hnène Medini; Bruno Manongiu; Neffati Aicha; Leila Chekir-Ghedira; Fethia Harzalla - Skhiri; Med Larbi Khouja

    2013-01-01

    Essential oils from Juniperus oxycedrus L. have been used since antiquity for fragrance, flavoring, medicinal, antimicrobial, insecticidal, and cosmetic purposes. Several works studied the chemical composition of the essential oils of Juniperus oxycedrus leaves. The aim of this study is to investigate the chemotaxonomic relationships and antibacterial activity of two Tunisian subspecies: Juniperus oxycedrus ssp. oxycedrus (L. K.) Deb. and Juniperus oxycedrus ssp. macrocarpa (S. & m.) Ball. In...

  20. Helianthus annuus ssp. texanus has chloroplast DNA and nuclear ribosomal RNA genes of Helianthus debilis ssp. cucumerifolius.

    OpenAIRE

    Rieseberg, L H; Beckstrom-Sternberg, S.; Doan, K

    1990-01-01

    Heiser [Heiser, C. B. (1951) Evolution 5, 42-51] hypothesized that Helianthus annuus ssp. texanus was derived by the introduction of H. annuus into Texas and subsequent introgression of genes from Helianthus debilis ssp. cucumerifolius into H. annuus. Although often considered to be one of the best cases of introgression in plants, alternative hypotheses to introgression, such as convergence or the joint retention of the ancestral condition, could not be ruled out in the original study. To te...

  1. Progeny of Eucalyptus globulus Labill ssp. Globulus

    Institute of Scientific and Technical Information of China (English)

    ZANGDao-qun; ZHANGChuan-hong; LUOJun-min; HUANGYong-xiang; ZHURen-gang

    2003-01-01

    The preliminary assessment of progeny test in a seed orchard of Eucalyptus globulus Labill.ssp.globulus at Yipinglang State Farm of Yunnan Province in China was conducted.The trial was composed of 21 replications,with 2 trees on each plot,15 plots in each incomplete block,and 18 incomplete blocks in each replication,laid out in a α-design of 270 open-pollinated families.The families were of three categories (natural populations,local land race and seed orchard).They were further subdivided into 11 regions.The data from an assessment of growth,stem form and other characteristics of the young trees after being planted 2 years were analyzed.Results are as follows:the different categories differed significantly in their growth,with the families from seed orchards being the best.Regions also differed significantly in their growth.The families from the regions of Westem Victoria,Eastern Victoria and all three seed orchards were better than others.The tamilies from Yunnan had the best frost tolerant and its survival was the highest.For growth,stem form and fungal tolerance,the families from Yunnan were similar to that from Southern Tasmania and Eastem Tasmania.Two-tree plots were strongly recommended over singletree plot designs for large-scale progeny trials.

  2. Multilocus sequence typing of Lactobacillus casei isolates from naturally fermented foods in China and Mongolia.

    Science.gov (United States)

    Bao, Qiuhua; Song, Yuqin; Xu, Haiyan; Yu, Jie; Zhang, Wenyi; Menghe, Bilige; Zhang, Heping; Sun, Zhihong

    2016-07-01

    Lactobacillus casei is a lactic acid bacterium used in manufacturing of many fermented food products. To investigate the genetic diversity and population biology of this food-related bacterium, 224 Lb. casei isolates and 5 reference isolates were examined by multilocus sequence typing (MLST). Among them, 224 Lb. casei isolates were isolated from homemade fermented foods, including naturally fermented dairy products, acidic gruel, and Sichuan pickles from 38 different regions in China and Mongolia. The MLST scheme was developed based on the analysis of 10 selected housekeeping genes (carB, clpX, dnaA, groEL, murE, pyrG, pheS, recA, rpoC, and uvrC). All 229 isolates could be allocated to 171 unique sequence types, including 25 clonal complexes and 71 singletons. The high index of association value (1.3524) and standardized index of association value (0.1503) indicate the formation of an underlying clonal population by all the isolates. However, split-decomposition, relative frequency of occurrence of recombination and mutation, and relative effect of recombination and mutation in the diversification values confirm that recombination may have occurred, and were more frequent than mutation during the evolution of Lb. casei. Results from Structure analyses (version 2.3; http://pritch.bsd.uchicago.edu/structure.html) demonstrated that there were 5 lineages in the Lb. casei isolates, and the overall relatedness built by minimum spanning tree showed no clear relationship between the clonal complexes with either the isolation sources or sampling locations of the isolates. Our newly developed MLST scheme of Lb. casei was an easy and valuable tool that, together with the construction of an MLST database, will contribute to further detailed studies on the evolution and population genetics of Lb. casei from various niches. PMID:27179867

  3. Antigenotoxic and Antimutagenic Activities of Probiotic Lactobacillus rhamnosus Vc against N-Methyl-N'-Nitro-N-Nitrosoguanidine.

    Science.gov (United States)

    Pithva, Sheetal P; Ambalam, Padma S; Ramoliya, Jignesh M; Dave, Jayantilal M; Vyas, Bharatkumar Rajiv Manuel

    2015-01-01

    The present study provides experimental evidence of in vivo reduction of genotoxic and mutagenic activities of potent carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) by the strain Lactobacillus rhamnosus Vc. In vitro studies revealed that coincubation of MNNG with viable cells of L. rhamnosus Vc resulted in the detoxification of the parent compound accompanied with reduction in genotoxicity (69%) and mutagenicity (61%) as evaluated by SOS-Chromotest and Ames test, respectively. Oral feeding of probiotic bacteria L. rhamnosus Vc (10(9) cfu) to Gallus gallus (chicks) for 30 days provided protection against MNNG-induced damage as evidenced from the significant decrease (P = 0.009) in glutathione S-transferase activity in the L. rhamnosus Vc+MNNG-treated chicks in comparison to the MNNG-treated chicks. Histopathology of colon and liver showed intact cells and mild inflammation in the L. rhamnosus Vc+MNNG-treated chicks, whereas heavy inflammation and degenerative changes were observed in MNNG-treated chicks. The results indicate that the probiotic L. rhamnosus Vc provided in vivo protection against MNNG-induced colon damage by detoxification of MNNG to less toxic metabolites. PMID:26312410

  4. Genome-scale reconstruction of metabolic networks of Lactobacillus casei ATCC 334 and 12A.

    Directory of Open Access Journals (Sweden)

    Elena Vinay-Lara

    Full Text Available Lactobacillus casei strains are widely used in industry and the utility of this organism in these industrial applications is strain dependent. Hence, tools capable of predicting strain specific phenotypes would have utility in the selection of strains for specific industrial processes. Genome-scale metabolic models can be utilized to better understand genotype-phenotype relationships and to compare different organisms. To assist in the selection and development of strains with enhanced industrial utility, genome-scale models for L. casei ATCC 334, a well characterized strain, and strain 12A, a corn silage isolate, were constructed. Draft models were generated from RAST genome annotations using the Model SEED database and refined by evaluating ATP generating cycles, mass-and-charge-balances of reactions, and growth phenotypes. After the validation process was finished, we compared the metabolic networks of these two strains to identify metabolic, genetic and ortholog differences that may lead to different phenotypic behaviors. We conclude that the metabolic capabilities of the two networks are highly similar. The L. casei ATCC 334 model accounts for 1,040 reactions, 959 metabolites and 548 genes, while the L. casei 12A model accounts for 1,076 reactions, 979 metabolites and 640 genes. The developed L. casei ATCC 334 and 12A metabolic models will enable better understanding of the physiology of these organisms and be valuable tools in the development and selection of strains with enhanced utility in a variety of industrial applications.

  5. Isolation and characterization of antimicrobial proteins produced by a potential probiotic strain of human Lactobacillus rhamnosus 231 and its effect on selected human pathogens and food spoilage organisms

    OpenAIRE

    Ambalam, P. S.; Prajapati, J. B.; Dave, J. M.; Nair, Baboo M.; Ljungh, Åsa; Vyas, B. R. M.

    2011-01-01

    Objective: To study in vitro properties of potential probiotics and the antimicrobial activity of Lactobacillus rhamnosus 231 isolated from human faeces. Methods and Results: Lact. rhamnosus 231 isolated from human faeces tolerated bile salt (4%), phenol (0.5%), and NaCl (4%) and retained viability at low pH (2.5). The cell-free culture (CFC) fi ltrate and extracellular protein concentrate (EPC) of Lact. rhamnosus 231 contained antimicrobial substances active against Pseudomonas aeruginosa, E...

  6. Draft Genome Sequence of Lactobacillus casei DPC6800, an Isolate with the Potential to Diversify Flavor in Cheese.

    Science.gov (United States)

    Stefanovic, Ewelina; Casey, Aidan; Cotter, Paul; Cavanagh, Daniel; Fitzgerald, Gerald; McAuliffe, Olivia

    2016-01-01

    Lactobacillus casei is a nonstarter lactic acid bacterium commonly present in various types of cheeses. It is believed that strains of this species have a significant impact on the development of cheese flavor. The draft genome sequence of L. casei DPC6800, isolated from a semi-hard Dutch cheese, is reported. PMID:26941145

  7. Effect of frequent consumption of a Lactobacillus casei-containing milk drink in Helicobacter pylori-colonized subjects.

    NARCIS (Netherlands)

    Cats, A.; Kuipers, E.J.; Bosschaert, MA; Pot, RG; Vandenbroucke-Grauls, C.M.J.E.; Kusters, J.G.

    2003-01-01

    BACKGROUND: Several studies have reported inhibitory effects of lactic acid bacteria on bacterial pathogens. AIM: To test whether a drink containing Lactobacillus casei strain Shirota inhibits Helicobacter pylori growth. METHODS: The in vitro growth inhibition of H. pylori was studied when L. casei

  8. Complete Genome Sequence of Lactobacillus casei Zhang, a New Probiotic Strain Isolated from Traditional Homemade Koumiss in Inner Mongolia, China▿

    Science.gov (United States)

    Zhang, Wenyi; Yu, Dongliang; Sun, Zhihong; Wu, Rina; Chen, Xia; Chen, Wei; Meng, He; Hu, Songnian; Zhang, Heping

    2010-01-01

    Lactobacillus casei Zhang is a new probiotic bacterium isolated from koumiss collected in Inner Mongolia, China. Here, we report the main genome features of L. casei Zhang and the identification of several predicted proteins implicated in interactions with the host. PMID:20675486

  9. Lactobacillus casei Zhang和Bifidobacterium lactis V9在益生菌酸乳中的应用%Application of Lactobacillus casei Zhang and Bifidobacterium lactis V9 in the probiotic yoghurt

    Institute of Scientific and Technical Information of China (English)

    刘彪; 张和平

    2012-01-01

    益生菌在酸乳中的应用已非常普遍,将Lactobacillus casei Zhang单独(样品A)以及与Bifidobacterium lactis V9复合(样品B),同酸乳发酵剂(G027)共同发酵益生菌酸乳,于4℃贮藏21 d.结果表明,整个贮藏期间2组样品间的黏度和持水性差异不显著;贮藏期间2组样品间L.casei Zhang的活菌数没有差异,且L.casei Zhang和B.lactis V9的活菌数不随贮藏时间而降低;L.casei Zhang和B.lactis V9复合益生菌酸奶感官评价优于单独添加L.casei Zhang酸乳.L.casei Zhang和B.lactis V9复合添加,更适合于益生菌酸乳的生产.%Now it is common for adding probiotics in fermented yoghurt. Yoghurt was fermented by Lactobadllus casei Zhang (A) or combined with Bifidobacterium lactis V9 (B) plus with starter culture (G027) and followed with a 21 d storage at 4 t. The results showed that there was no significance between A and B concerning viscosity and syneresis. It also observed no significant difference existed in counts of L. casei 7ian% between A and B during storage and counts of two probiotics had no change as the storage passed by. However, Fermented milk by combined L. casei Zhang with B. lactis V9 possess markedly higher favorable sensory scores than L. casei Zhang fermented milk, suggesting that it is more suitable for probiotic yoghurt manufacture.

  10. Safety Study of an Antimicrobial Peptide Lactocin 160, Produced by the Vaginal Lactobacillus rhamnosus

    OpenAIRE

    Dover, Sara E.; Alla A. Aroutcheva; S. Faro; Chikindas, Michael L.

    2007-01-01

    Objective. To evaluate the safety of the antimicrobial peptide, lactocin 160. Methods. Lactocin 160, a product of vaginal probiotic Lactobacillus rhamnosus 160 was evaluated for toxicity and irritation. An in vitro human organotypic vaginal-ectocervical tissue model (EpiVaginal) was employed for the safety testing by determining the exposure time to reduce tissue viability to 50% (ET-50). Hemolytic activity of lactocin160 was tested using 8% of human erythrocyte suspens...

  11. Lactobacillus rhamnosus GG-supplemented formula expands butyrate-producing bacterial strains in food allergic infants

    OpenAIRE

    Berni Canani, Roberto; Sangwan, Naseer; Stefka, Andrew T.; Nocerino, Rita; Paparo, Lorella; Aitoro, Rosita; Calignano, Antonio; Khan, Aly A.; Gilbert, Jack A.; Nagler, Cathryn R.

    2015-01-01

    Dietary intervention with extensively hydrolyzed casein formula supplemented with Lactobacillus rhamnosus GG (EHCF+LGG) accelerates tolerance acquisition in infants with cow's milk allergy (CMA). We examined whether this effect is attributable, at least in part, to an influence on the gut microbiota. Fecal samples from healthy controls (n=20) and from CMA infants (n=19) before and after treatment with EHCF with (n=12) and without (n=7) supplementation with LGG were compared by 16S rRNA-based ...

  12. Estudio etnobotánico, arquitectura foliar y anatomía vegetativa de Agastache mexicana ssp. mexicana y A. mexicana ssp. xolocotziana Etnobotany, leaf architecture, and vegetative anatomy of Agastache mexicana ssp. mexicana and A. mexicana ssp. xolocotziana

    OpenAIRE

    Magali A. Santillán-Ramírez; Ma. Edith López-Villafranco; Silvia Aguilar-Rodríguez; Abigail Aguilar-Contreras

    2008-01-01

    El presente trabajo incluye información etnobotánica, así como la arquitectura foliar y anatomía vegetativa de Agastache mexicana ssp. mexicana (toronjil morado) y A. mexicana ssp. xolocotziana (toronjil blanco), con la finalidad de que sean incluidas en la Farmacopea Herbolaria de los Estados Unidos Mexicanos (FHEUM) y que sus caracteres anatómicos se utilicen para validar la identidad del material vegetal en el control de calidad. El registro de la información etnobotánica se efectuó por me...

  13. [Flavonoids of Artemisia campestris, ssp. glutinosa].

    Science.gov (United States)

    Hurabielle, M; Eberle, J; Paris, M

    1982-10-01

    Four flavanones (pinostrobin, pinocembrin, sakuranetin and naringenin), one dihydroflavonol (7-methyl aromadendrin) and one flavone (hispidulin) have been isolated from Artemisia campestris L. ssp. glutinosa Gay and identified by spectroscopic methods. Artemisia campestris L. sous-espèce glutinosa Gay est une Composée Anthémidée largement répandue sur les sables du littoral méditerranéean et abondante dans certaines régions d'Espagne et d'Italie. Dans le cadre d'une étude chimiotaxonomique du genre Artemisia Tourn., nous nous sommes intéressés à l'analyse des flavonoïdes, composés jamais décrits, à notre connaissance, dans cette espèce d' Artemisia. Les sommités fleuries d' Artemisia campestris sous-espèce glutinosa, séchées et pulvérisées, sont dégraissées à l'ether de pétrole et épuisées par le chloroforme. Le fractionnement de l'extrait chloroformique, par chromatographie sur colonne de silice, et la purification de certaines fractions conduisent à l'isolement de six génines flavoniques, à l'etat pur. L' étude des spectres UV, des spectres de masse et des spectres de RMN [1,2] et la comparaison avec des échantillons authentiques permettent de proposer, pour ces flavonoïdes, les structures de la pinostrobine [3], de la pinocembrine [4], de la sakuranétine, de la naringénine [5] (flavanones), de la méthyl-7-aromadendrine, [6, 7] (dihydroflavonol) et de l'hispiduline [8, 9] (flavone); quatre de ces génines sont méthylées. Parmi ces flavonoïdes, la pinostrobine n'a jamais été décrite, à notre connaissance, dans la famille des Composées; la pinocembrine, la sakuranétine et la naringénine ont déjà été signalées chez quelques Astéracées et Eupatoriées [10], et l'hispiduline dans la tribu des Anthémidées ( Santolina chamaecyparissus L.) [8]. Seule, la méthyl-7-aromadendrine semble décrite, à ce jour, dans le genre Artemisia Tourn. [7].

  14. [Flavonoids of Artemisia campestris, ssp. glutinosa].

    Science.gov (United States)

    Hurabielle, M; Eberle, J; Paris, M

    1982-10-01

    Four flavanones (pinostrobin, pinocembrin, sakuranetin and naringenin), one dihydroflavonol (7-methyl aromadendrin) and one flavone (hispidulin) have been isolated from Artemisia campestris L. ssp. glutinosa Gay and identified by spectroscopic methods. Artemisia campestris L. sous-espèce glutinosa Gay est une Composée Anthémidée largement répandue sur les sables du littoral méditerranéean et abondante dans certaines régions d'Espagne et d'Italie. Dans le cadre d'une étude chimiotaxonomique du genre Artemisia Tourn., nous nous sommes intéressés à l'analyse des flavonoïdes, composés jamais décrits, à notre connaissance, dans cette espèce d' Artemisia. Les sommités fleuries d' Artemisia campestris sous-espèce glutinosa, séchées et pulvérisées, sont dégraissées à l'ether de pétrole et épuisées par le chloroforme. Le fractionnement de l'extrait chloroformique, par chromatographie sur colonne de silice, et la purification de certaines fractions conduisent à l'isolement de six génines flavoniques, à l'etat pur. L' étude des spectres UV, des spectres de masse et des spectres de RMN [1,2] et la comparaison avec des échantillons authentiques permettent de proposer, pour ces flavonoïdes, les structures de la pinostrobine [3], de la pinocembrine [4], de la sakuranétine, de la naringénine [5] (flavanones), de la méthyl-7-aromadendrine, [6, 7] (dihydroflavonol) et de l'hispiduline [8, 9] (flavone); quatre de ces génines sont méthylées. Parmi ces flavonoïdes, la pinostrobine n'a jamais été décrite, à notre connaissance, dans la famille des Composées; la pinocembrine, la sakuranétine et la naringénine ont déjà été signalées chez quelques Astéracées et Eupatoriées [10], et l'hispiduline dans la tribu des Anthémidées ( Santolina chamaecyparissus L.) [8]. Seule, la méthyl-7-aromadendrine semble décrite, à ce jour, dans le genre Artemisia Tourn. [7]. PMID:17396957

  15. Suitability of cereal porridges as substrate for probiotic strain Lactobacillus rhamnosus GG

    Directory of Open Access Journals (Sweden)

    Ľubomír Valík

    2013-02-01

    Full Text Available The aim of this work was to find new substrates suitable for growth and metabolism of probiotic strain Lactobacillus rhamnosus GG, which would be interesting for development of new functional food. The growth and metabolic activity of Lb. rhamnosus GG in cereal (rye, barley, oat and millet porridges were monitored during fermentation process. Cereal and porridges, were inoculated with this strain at two initial levels to obtain approximately 5 or 6 log colony form units per gram of suspension after sterilization and cooling,. Fermentation was led stationary at 37 °C for 48 hours and viable cell count, pH value, titratable acidity and organic acids were analysed. Metabolic activity of Lb. rhamnosus GG was influenced by inoculation level and by the type of cereal used. The cereals fermented by lactic acid bacteria, especially probiotic strains, might broaden the offer of probiotic products for those suffering from milk allergy.doi:10.5219/242 Normal 0 21 false false false SK X-NONE X-NONE

  16. Post-traumatic endophthalmitis due to Brevibacterium casei : A case report

    Directory of Open Access Journals (Sweden)

    Asima Banu

    2013-02-01

    Full Text Available Endophthalmitis is a serious post-traumatic ocular complication that can lead to loss of vision. We report a case of acute post-traumatic endophthalmitis following a penetrating injury caused by an unusual organism, Brevibacterium casei . The patient was successfully treated with intravitreal antibiotics like ceftazidime and vancomycin, along with topical cefazolin and tobramycin. Brevibacterium casei can be added to the list of rare bacteria causing endophthalmitis and should be kept in mind by clinicians as a potential source of pathology.

  17. Engineering sorbitol-6-phosphate Dehydrogenase encoding gene in the lactose operon of Lactobacillus casei.

    OpenAIRE

    Nissen, Lorenzo; Yebra, María Jesús; Sgobarti, Barbara; Pérez Martínez, Gaspar

    2005-01-01

    Sorbitol is a sugar polyol claimed to have health-promoting properties. D-sorbitol-6-phosphate dehydrogeanse (StolPDh) is required for sorbose and sorbitol metabolism in Lactobacillus casei. StolPDh catalyzes the oxidation of Sorbitol-6-phosphate and also the reverse reaction, or reather, the reduction of fructose-6-phosphate with NAD+ regeneration. In order to test this function in vivo, a new food grade recombinant strain of L. casei was constructed by the integration of a StolPDh-encodi...

  18. Lactobacillus casei reduces susceptibility to type 2 diabetes via microbiota-mediated body chloride ion influx

    OpenAIRE

    Yong Zhang; Xiao Guo; Jianlin Guo; Qiuwen He; He Li; Yuqin Song; Heping Zhang

    2014-01-01

    Gut microbiota mediated low-grade inflammation is involved in the onset of type 2 diabetes (T2DM). In this study, we used a high fat sucrose (HFS) diet-induced pre-insulin resistance and a low dose-STZ HFS rat models to study the effect and mechanism of Lactobacillus casei Zhang in protecting against T2DM onset. Hyperglycemia was favorably suppressed by L. casei Zhang treatment. Moreover, the hyperglycemia was connected with type 1 immune response, high plasma bile acids and urine chloride io...

  19. Inhibitory effect of Lactobacillus rhamnosus on pathogenic bacteria isolated from women with bacterial vaginosis

    Directory of Open Access Journals (Sweden)

    Gita Eslami

    2014-06-01

    Full Text Available Background: Considering the high prevalence of bacterial vaginosis and its association with urinary tract infection in women and treatment of gynecologic problems occur when a high recurrence of bacterial vaginosis is often treated with antibiotics. The purpose of this study is to investigate the inhibitory effect of Lactobacillus rhamnosus on pathogenic bacteria isolated from women with bacterial vaginosis, respectively.Materials and Methods: 96 samples from women with bacterial vaginosis discharge referred to health centers dependent Shahid Beheshti University in 91-92 were taken by a gynecologist with a dacron swab and put in sterile tubes containing TSB broth and Thioglycollate broth and were immediately sent to the lab location in cold chain for the next stages of investigation. From Thioglycollate and TSB medium was cultured on blood agar and EMB and Palkam and Differential diagnosis environments, and then incubated for 24 h at 37°C. Strains of Lactobacillus rhamnosus were cultured in MRSA environment and were transfered to the lab. After purification of pathogenic bacteria, MIC methods and antibiogram, Lactobacillus rhamnosus inhibitory effect on pathogenic bacteria is checked. Statistical analysis was done by SPSS software v.16.Results: The results of this study show the inhibitory effect of Lactobacillus rhamnosus on some pathogenic bacteria that cause bacterial vaginosis, including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Streptococcus agalactiae, Entrococcus, Listeria monocytogenes and E.Coli. Microscopic examination of stained smears of the large number of Lactobacillus and pathogenic bacteria showed reduced. The prevalence of abnormal vaginal discharge, history of drug use means of preventing pregnancy and douching, respectively, 61%, 55%, 42% and 13% respectively. Significant difference was observed between the use and non-use of IUD in women with bacterial vaginosis infection

  20. Galanthindole: a new indole alkaloid from Galanthus plicatus ssp. byzantinus.

    Science.gov (United States)

    Unver, Nehir; Kaya, G Irem; Werner, Christa; Verpoorte, Robert; Gözler, Belkis

    2003-09-01

    A new indole alkaloid, galanthindole, was isolated from Galanthus plicatus ssp. byzantinus (Amaryllidaceae), a plant native to northwestern Turkey. Incorporating a non-fused indole ring, galanthindole may represent the prototype of a new subgroup of the Amaryllidaceae alkaloids. Two other bases, (+)-11-hydroxyvittatine and hordenine, are also reported from the same plant.

  1. Incorporação de Lactobacillus casei microencapsulado em queijo tipo coalho = Incorporation of encapsulated Lactobacillus casei into type curd cheese

    Directory of Open Access Journals (Sweden)

    Lima, Janilton Rodrigues

    2014-01-01

    Conclusão: Desta forma, os queijos obtidos com a introdução de ambas as formas de Lactobacillus casei apresentaram concentrações de células viáveis consideradas adequadas para alimentos probióticos, podendo concluir que o queijo tipo coalho é um produto com características interessantes para a veiculação de micro-organismos probióticos

  2. 益生菌Lactobacillus casei Zhang增殖培养基的优化%Study on the Optimization of Enrichment Medium of Lactobacillus casei Zhang

    Institute of Scientific and Technical Information of China (English)

    高鹏飞; 李妍; 赵文静; 陈霞; 崔景丽; 张磊; 张和平

    2008-01-01

    Lactobacillus casei Zhang 是一株分离自传统酸马奶中的益生菌.本文研究了不同碳源、氮源、碳氮比例、微量元素及缓冲盐对 Lactobacillus casei Zhang 增殖培养的效果,并采用响应面法对优选的碳源、氮源和缓冲盐类的组成含量进行优化,得到 Lactobacillus casei Zhang 的增殖培养基为:葡萄糖20.9 g/L、大豆蛋白胨10.45 g/L、酵母粉10.45 g/L、K2HPO4 3.5 g/L、醋酸钠14.6 g/L、柠檬酸钠2.3 g/L、MgSO4·7H2O 1 g/L,MnSO4·5H2O 54 mg/L、CuSO4·5H2O 10 mg/L、吐温80为1 g/L.Lactobacillus casei Zhang 在此增殖培养基中经37℃ 18 h培养活茵数可达到4.78×109 CFU/mL,比在MRS中(4.8×108 CFU/mL)提高近10倍.

  3. BEBIDA FERMENTADA DE SUERO DE QUESO FRESCO INOCULADA CON Lactobacillus casei FERMENTED FRESH CHEESE MILKWHEY BEVERAGE INOCULATED WITH Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Margarita María Londoño Uribe

    2008-06-01

    Full Text Available Este trabajo tuvo por objetivo desarrollar una bebida fermentada de suero de queso fresco inoculada con Lactobacillus casei, a la cual se le evaluó la viabilidad del microorganismo, utilizando medios de cultivo selectivos, bajo condiciones anaeróbicas y, luego, se procedió a verificar su resistencia a los ácidos gástricos y sales biliares, simulando así, las condiciones del tracto gastrointestinal de los humanos. Para verificar la supervivencia durante el período de almacenamiento (21 días, la cepa, se caracterizó morfológica y bioquímicamente. Adicionalmente, se llevó a cabo la prueba de aceptabilidad, evaluando la bebida con 80 jueces. Se realizaron análisis físico-químicos, microbiológicos y sensoriales, a la materia prima y al producto elaborado, acorde a las normas vigentes en Colombia. La bebida fue saborizada con pulpa de maracuyá (Passi flora edulis. Se obtuvieron recuentos de viabilidad a pH 2,0, en agar MRS de 5,38∙10(7ufc∙g-1 y 1,3∙10(6ufc∙g-1 y en agar M17 de 6,96∙10(7ufc∙g-1 y de 1,16∙10(6ufc∙g-1, en los días 1 y 21, respectivamente. A pH 7,0, en agar MRS, se registraron valores de 3,37 ∙10(7ufc∙g-1 y 1.56 ∙10(6ufc∙g-1 y en agar M17 de 8,85∙10(7ufc∙g-1 y de 1,82∙10(6ufc∙g-1, en los días 1 y 21, respectivamente. La bebida desarrollada, tuvo una aceptación de “me gusta”, y presentó una vida de anaquel de hasta 21 días.The objective of this research was to develop a fermented fresh cheese milkwhey beverage inoculated with Lactobacillus casei, to which the viability of microorganism was evaluated using selective cultivation means under anaerobic conditions and then it was verified its biliary resistance to gastric acids and salts, simulating this way, the conditions of gastrointestinal tract of humans. To verify the survival during the period of storage (21 days, the stump was characterized morphological and bio-chemically. Additionally the test of acceptability was carried out

  4. 益生菌Lactobacillus casei Zhang固态发酵条件的优化%Optimization of solid state fermentation conditions for Lactobacillus casei Zhang

    Institute of Scientific and Technical Information of China (English)

    崔景丽; 张磊; 高鹏飞; 王计成; 孙天松; 张和平

    2009-01-01

    利用一株分离自传统发酵酸马奶中的益生干酪乳杆菌(Lactobacillus casei Zhang) 进行固态发酵(Solid State Fermentation,SSF).以发酵物中的活菌数为主要指标,采用 九因素四水平(L32(49))的正交试验优化固态发酵培养基,并在优化的培养基基础上研究不同的初始含水量及培养时间对Lactobacillus casei Zhang活菌数的影响.实验结果表明,在固态发酵培养基组成为4g豆粕、5g麸皮、0.6g乳清粉、0.3g葡萄糖、 0.3g碳酸钙、0.02g硫酸铵、0.01g硫酸镁,初始含水量为55%的优化条件下,37℃发酵60h, 发酵物中Lactobacillus casei Zhang活菌数可达到4.08×1010CFU/g.

  5. PCR method for detection and identification of Lactobacillus casei/paracasei bacteriophages in dairy products.

    Science.gov (United States)

    Binetti, Ana G; Capra, M Luján; Alvarez, Miguel A; Reinheimer, Jorge A

    2008-05-31

    Bacteriophage infections of starter lactic acid bacteria (LAB) pose a serious risk to the dairy industry. Nowadays, the expanding use of valuable Lactobacillus strains as probiotic starters determines an increase in the frequency of specific bacteriophage infections in dairy plants. This work describes a simple and rapid Polymerase Chain Reaction (PCR) method that detects and identifies bacteriophages infecting Lactobacillus casei/paracasei, the main bacterial species used as probiotic. Based on a highly conserved region of the NTP-binding genes belonging to the replication module of L. casei phages phiA2 and phiAT3 (the only two whose genomes are completely sequenced), a pair of primers was designed to generate a specific fragment. Furthermore, this PCR detection method proved to be a useful tool for monitoring and identifying L. casei/paracasei phages in industrial samples since specific PCR signals were obtained from phage contaminated milk (detection limit: 10(4) PFU/mL milk) and other commercial samples (fermented milks and cheese whey) that include L. casei/paracasei as probiotic starter (detection limit: 10(6) PFU/mL fermented milk). Since this method can detect the above phages in industrial samples and can be easily incorporated into dairy industry routines, it might be readily used to earmark contaminated milk for use in processes that do not involve susceptible starter organisms, or processes which involve phage-deactivating conditions. PMID:18471918

  6. Genome Sequence Analysis of the Biogenic Amine-Degrading Strain Lactobacillus casei 5b

    OpenAIRE

    Ladero Losada, Víctor Manuel; Herrero, Ana; Martínez Álvarez, Noelia; Río Lagar, Beatriz del; Linares, Daniel M.; Fernández García, María; Martín, M. Cruz; Álvarez González, Miguel Ángel

    2014-01-01

    We here report a 3.02-Mbp annotated draft assembly of the Lactobacillus casei 5b genome. The sequence of this biogenic amine-degrading dairy isolate may help identify the mechanisms involved in the catabolism of biogenic amines and perhaps shed light on ways to reduce the presence of these toxic compounds in food.

  7. CASEI Project (Consultation and Administration Specialists in Early Intervention) Final Report.

    Science.gov (United States)

    Ostrosky, Michaelene M.

    This final report describes the activities and accomplishments of the Consultation and Administration Specialists in Early Intervention Project (CASEI). This federally funded project was developed to provide cross-disciplinary preservice training for early intervention (EI) specialists in Illinois. Students were recruited from a broad range of…

  8. Ca2+-Citrate Uptake and Metabolism in Lactobacillus casei ATCC 334

    NARCIS (Netherlands)

    Mortera, Pablo; Pudlik, Agata; Magni, Christian; Alarcon, Sergio; Lolkema, Juke S.

    2013-01-01

    The putative citrate metabolic pathway in Lactobacillus casei ATCC 334 consists of the transporter CitH, a proton symporter of the citrate-divalent metal ion family of transporters CitMHS, citrate lyase, and the membrane-bound oxaloacetate decarboxylase complex OAD-ABDH. Resting cells of Lactobacill

  9. Complete Genome Sequence of the Probiotic Bacterium Lactobacillus casei LC2W▿

    OpenAIRE

    Chen, Chen; Ai, Lianzhong; Zhou, Fangfang; Wang, Lei; Zhang, Hao; Chen, Wei(Department of Physics, State Key Laboratory of Nuclear Physics and Technology, Peking University, Beijing, 100871, China); Guo, Benheng

    2011-01-01

    Lactobacillus casei LC2W, a patented probiotic strain (Z. Wu, European patent EP 1642963 B1, February 2009), has been isolated from Chinese traditional dairy products and implemented in industrial production as starter culture. Here we present the complete genome sequence of LC2W and the identification of a gene cluster implicated in the biosynthesis of exopolysaccharides.

  10. Complete Genome Sequence of the Probiotic Strain Lactobacillus casei BD-II ▿

    OpenAIRE

    Ai, Lianzhong; Chen, Chen; Zhou, Fangfang; Wang, Lei; Zhang, Hao; Chen, Wei(Department of Physics, State Key Laboratory of Nuclear Physics and Technology, Peking University, Beijing, 100871, China); Guo, Benheng

    2011-01-01

    Lactobacillus casei BD-II, a patented probiotic strain (U.S. patent 7,270,994 B2), was isolated from homemade koumiss in China and has been implemented in the industrial production as starter cultures. Here we report the complete genome sequence of BD-II, which shows high similarity with the well-studied probiotic BL23.

  11. Chemical and Antibacterial Polymorphism of Juniperus oxycedrus ssp. oxycedrus and Juniperus oxycedrus ssp. macrocarpa (Cupressaceae Leaf Essential Oils from Tunisia

    Directory of Open Access Journals (Sweden)

    Hnène Medini

    2013-01-01

    Full Text Available Essential oils from Juniperus oxycedrus L. have been used since antiquity for fragrance, flavoring, medicinal, antimicrobial, insecticidal, and cosmetic purposes. Several works studied the chemical composition of the essential oils of Juniperus oxycedrus leaves. The aim of this study is to investigate the chemotaxonomic relationships and antibacterial activity of two Tunisian subspecies: Juniperus oxycedrus ssp. oxycedrus (L. K. Deb. and Juniperus oxycedrus ssp. macrocarpa (S. & m. Ball. In addition, and for the first time, we reported the antibacterial activities of Tunisian J. oxycedrus ssp. macrocarpa and J. oxycedrus ssp. oxycedrus against four bacteria. Essential oils obtained by hydrodistillation were analysed by GC and GC/MS. Fifty-five constituents were identified. Thirty four major compounds were retained for the study of the chemical variability, and α-pinene, sylvestrene, p-cymene, and 13-epi-manoyl oxide were the main ones. The chemical principal components analysis (PCA identified three chemotypes. The study of the antibacterial activity showed that Escherichia coli was found to be extremely resistant (zone diameter 0 mm to all the oils tested, while Staphylococcus aureus was the most sensitive strain (zone diameter 13.5 mm and MIC ranged from 600 to 650 μg/mL.

  12. Heterologous expression of mannanase and developing a new Reporter gene system in Lactobacillus casei and Escherichia coli

    DEFF Research Database (Denmark)

    Lin, Jinzhong; Zou, Yexia; Ma, Chengjie;

    2015-01-01

    into the expression vector pELX1. The expression patterns of these reporter genes in Lactobacillus casei were investigated by measuring their enzymatic activities and estimating their recombinant protein yields using western blot analysis. Whereas mannanase activity was positively correlated with the accumulation...... to use the more stable mannanase gene as the reporter to test secretion expression in L. casei. Two pELX1-based secretion vectors were constructed: one carried the signal peptide of the unknown secretion protein Usp45 from Lactococcus lactis (pELSH), and the other contained the full-length SlpA protein....... casei and E.coli....

  13. Inhibitory effect of essential oils against Lactobacillus rhamnosus and starter culture in fermented milk during its shelf-life period

    Directory of Open Access Journals (Sweden)

    Cristiane Mengue Feniman Moritz

    2012-09-01

    Full Text Available The use of essential oils in foods has attracted great interest, due to their antagonistic action against pathogenic microorganisms. However, this action is undesirable for probiotic foods, as products containing Lactobacillus rhamnosus. The aim of the present study was to measure the sensitivity profile of L. rhamnosus and a yogurt starter culture in fermented milk, upon addition of increasing concentrations of cinnamon, clove and mint essential oils. Essential oils were prepared by steam distillation, and chemically characterised by gas chromatography-mass spectrometry (GC-MS and determination of density. Survival curves were obtained from counts of L. rhamnosus and the starter culture (alone and in combination, upon addition of 0.04% essential oils. In parallel, titratable acidity was monitored over 28 experimental days. Minimum inhibitory concentration values, obtained using the microdilution method in Brain Heart Infusion medium, were 0.025, 0.2 and 0.4% for cinnamon, clove and mint essential oils, respectively. Cinnamon essential oil had the highest antimicrobial activity, especially against the starter culture, interfering with lactic acid production. Although viable cell counts of L. rhamnosus were lower following treatment with all 3 essential oils, relative to controls, these results were not statistically significant; in addition, cell counts remained greater than the minimum count of 10(8CFU/mL required for a product to be considered a probiotic. Thus, although use of cinnamon essential oil in yogurt makes starter culture fermentation unfeasible, it does not prevent the application of L. rhamnosus to probiotic fermented milk. Furthermore, clove and mint essential oil caused sublethal stress to L. rhamnosus.

  14. The Variable Regions of Lactobacillus rhamnosus Genomes Reveal the Dynamic Evolution of Metabolic and Host-Adaptation Repertoires.

    Science.gov (United States)

    Ceapa, Corina; Davids, Mark; Ritari, Jarmo; Lambert, Jolanda; Wels, Michiel; Douillard, François P; Smokvina, Tamara; de Vos, Willem M; Knol, Jan; Kleerebezem, Michiel

    2016-01-01

    Lactobacillus rhamnosus is a diverse Gram-positive species with strains isolated from different ecological niches. Here, we report the genome sequence analysis of 40 diverse strains of L. rhamnosus and their genomic comparison, with a focus on the variable genome. Genomic comparison of 40 L. rhamnosus strains discriminated the conserved genes (core genome) and regions of plasticity involving frequent rearrangements and horizontal transfer (variome). The L. rhamnosus core genome encompasses 2,164 genes, out of 4,711 genes in total (the pan-genome). The accessory genome is dominated by genes encoding carbohydrate transport and metabolism, extracellular polysaccharides (EPS) biosynthesis, bacteriocin production, pili production, the cas system, and the associated clustered regularly interspaced short palindromic repeat (CRISPR) loci, and more than 100 transporter functions and mobile genetic elements like phages, plasmid genes, and transposons. A clade distribution based on amino acid differences between core (shared) proteins matched with the clade distribution obtained from the presence-absence of variable genes. The phylogenetic and variome tree overlap indicated that frequent events of gene acquisition and loss dominated the evolutionary segregation of the strains within this species, which is paralleled by evolutionary diversification of core gene functions. The CRISPR-Cas system could have contributed to this evolutionary segregation. Lactobacillus rhamnosus strains contain the genetic and metabolic machinery with strain-specific gene functions required to adapt to a large range of environments. A remarkable congruency of the evolutionary relatedness of the strains' core and variome functions, possibly favoring interspecies genetic exchanges, underlines the importance of gene-acquisition and loss within the L. rhamnosus strain diversification. PMID:27358423

  15. Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis

    NARCIS (Netherlands)

    Kajikawa, A.; Satoh, E.; Leer, R.J.; Yamamoto, S.; Igimi, S.

    2007-01-01

    A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization

  16. Parâmetros de produção de leite de búfala fermentado por Lactobacillus casei Production parameters of buffalo milk fermented by Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Cláudia Puerari Faria

    2006-03-01

    Full Text Available O leite de búfala foi fermentado por Lactobacillus casei, com diferentes concentrações de açúcar e tempos de fermentação, e estocado durante 30 dias a 5 e 10°C. Avaliaram-se a acidez, o pH e a viabilidade de L. casei nos diferentes tratamentos. O leite fermentado por 18 horas não apresentou os parâmetros requeridos para o produto, enquanto os fermentados por 22 e 24 horas apresentaram acidez e pH adequados. O tempo e a temperatura de estocagem influenciaram esses parâmetros. A viabilidade de L. casei inicial foi maior que 9 log UFC mL-1 e a final, maior que 8 log UFC mL-1, com influência da acidez.Buffalo milk fermented by Lactobacillus casei was elaborated with different sugar concentrations and fermentation times, and stored during 30 days at 5 and 10°C. Acidity, pH and L. casei viability were evaluated in the different treatments. Milk fermented for 18 hours did not show adequate parameters for this product. Milk fermented for 22 and 24 hours presented adequate acidity and pH. Storage time and temperature influenced these parameters. The L. casei initial viability was greater than 9 log CFU mL-1 and the final was greater than 8 log CFU mL-1, with influence from acidity.

  17. Structure of an extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83

    International Nuclear Information System (INIS)

    The extracellular polysaccharide produced by Lactobacillus rhamnosus strain C83 was found to be composed of d-glucose and d-galactose in a molar ratio of 2:3. The primary structure of the polysaccharide was shown by sugar analysis, methylation analysis, FABMS, partial acid hydrolysis and nuclear magnetic resonance (NMR) spectroscopy to consist of a pentasaccharide repeating unit having the following structure:-3)-α-d-Glcp-(1-2)-β-d-Galf-(1-6)-α-d-Galp-(1-6) -α-d-Glcp-(1-3)-β-d-Galf-(1-. (Copyright (c) 1998 Elsevier Science B.V., Amsterdam. All rights reserved.)

  18. Estudio etnobotánico, arquitectura foliar y anatomía vegetativa de Agastache mexicana ssp. mexicana y A. mexicana ssp. xolocotziana Etnobotany, leaf architecture, and vegetative anatomy of Agastache mexicana ssp. mexicana and A. mexicana ssp. xolocotziana

    Directory of Open Access Journals (Sweden)

    Magali A. Santillán-Ramírez

    2008-12-01

    Full Text Available El presente trabajo incluye información etnobotánica, así como la arquitectura foliar y anatomía vegetativa de Agastache mexicana ssp. mexicana (toronjil morado y A. mexicana ssp. xolocotziana (toronjil blanco, con la finalidad de que sean incluidas en la Farmacopea Herbolaria de los Estados Unidos Mexicanos (FHEUM y que sus caracteres anatómicos se utilicen para validar la identidad del material vegetal en el control de calidad. El registro de la información etnobotánica se efectuó por medio de entrevistas semiestructuradas a pobladores del municipio de Temoaya, Estado de México, México. Para el estudio anatómico y arquitectura foliar, se elaboraron preparaciones semipermanentes y permanentes de tallos y hojas. Ambos toronjiles son utilizados por los habitantes de la zona de estudio como recurso terapéutico y de ornato; los distinguen por la forma de las hojas, el color de la flor y el aroma, pero no existen preferencias de uso entre ambos. La arquitectura foliar sigue el patrón acródromo de posición basal con un desarrollo imperfecto; las areolas muestran un desarrollo imperfecto con disposición al azar, con vénulas simples o ramificadas una vez y el margen con dientes cunonioides en ambas especies. Diferencias anatómicas se observan en el tallo y son los tricomas no glandulares uniseriados en mayor abundancia y las bandas de esclerénquima más desarrolladas los caracteres que distinguen a A. mexicana ssp. xolocotziana de A. mexicana ssp. mexicana.This work provides information on ethnobotany, leaf architecture and anatomy of Agastache mexicana ssp. mexicana (toronjil morado and A. mexicana ssp. xolocotziana (toronjil blanco, with the purpose that they will be included in the Pharmacopeia Herbolaria of the Estados Unidos Mexicanos (FHEUM and that their anatomical characters could be used to validate the identity of the botanical material in quality control. We gathered ethnobotanical information using semi

  19. Flavonoids from Ulex airensis and Ulex europaeus ssp. europaeus.

    Science.gov (United States)

    Máximo, Patrícia; Lourenço, Ana; Feio, Sónia Savluchinske; Roseiro, José Carlos

    2002-02-01

    From the dichloromethane extract of Ulex airensis three new isoflavonoids, ulexin C (1), ulexin D (2), and 7-O-methylisolupalbigenin (3), were isolated and characterized by spectroscopic methods. Ulexin D (2) was also identified from the dichloromethane extract of Ulex europaeus ssp. europaeus. Together with these new metabolites, 18 compounds of previously known structures were isolated and identified from both species. The antifungal activity of these compounds was tested against Cladosporium cucumerinum by a bioautographic TLC assay. PMID:11858751

  20. Optimal second order diagonally implicit SSP Runge--Kutta methods

    OpenAIRE

    Kocsis, Tihamér A.; Németh, Adrián

    2014-01-01

    Optimal Strong Stability Preserving (SSP) Runge--Kutta methods has been widely investegated in the last decade and many open conjectures have been formulated. The iterated implicit midpoint rule has been observed numerically optimal in large classes of second order methods, and was proven to be optimal for some small cases, but no general proof was known so far to show its optimality. In this paper we show a new approach to analytically investigate this problem and determine the unique optima...

  1. Expression of bifidobacterial phytases in Lactobacillus casei and their application in a food model of whole-grain sourdough bread.

    Science.gov (United States)

    García-Mantrana, Izaskun; Yebra, María J; Haros, Monika; Monedero, Vicente

    2016-01-01

    Phytases are enzymes capable of sequentially dephosphorylating phytic acid to products of lower chelating capacity and higher solubility, abolishing its inhibitory effect on intestinal mineral absorption. Genetic constructions were made for expressing two phytases from bifidobacteria in Lactobacillus casei under the control of a nisin-inducible promoter. L. casei was able of producing, exporting and anchoring to the cell wall the phytase of Bifidobacterium pseudocatenulatum. The phytase from Bifidobacterium longum spp. infantis was also produced, although at low levels. L. casei expressing any of these phytases completely degraded phytic acid (2mM) to lower myo-inositol phosphates when grown in MRS medium. Owing to the general absence of phytase activity in lactobacilli and to the high phytate content of whole grains, the constructed L. casei strains were applied as starter in a bread making process using whole-grain flour. L. casei developed in sourdoughs by fermenting the existing carbohydrates giving place to an acidification. In this food model system the contribution of L. casei strains expressing phytases to phytate hydrolysis was low, and the phytate degradation was mainly produced by activation of the cereal endogenous phytase as a consequence of the drop in pH. This work shows the capacity of lactobacilli to be modified in order to produce enzymes with relevance in food technology processes. The ability of these strains in reducing the phytate content in fermented food products must be evaluated in further models.

  2. Expression of bifidobacterial phytases in Lactobacillus casei and their application in a food model of whole-grain sourdough bread.

    Science.gov (United States)

    García-Mantrana, Izaskun; Yebra, María J; Haros, Monika; Monedero, Vicente

    2016-01-01

    Phytases are enzymes capable of sequentially dephosphorylating phytic acid to products of lower chelating capacity and higher solubility, abolishing its inhibitory effect on intestinal mineral absorption. Genetic constructions were made for expressing two phytases from bifidobacteria in Lactobacillus casei under the control of a nisin-inducible promoter. L. casei was able of producing, exporting and anchoring to the cell wall the phytase of Bifidobacterium pseudocatenulatum. The phytase from Bifidobacterium longum spp. infantis was also produced, although at low levels. L. casei expressing any of these phytases completely degraded phytic acid (2mM) to lower myo-inositol phosphates when grown in MRS medium. Owing to the general absence of phytase activity in lactobacilli and to the high phytate content of whole grains, the constructed L. casei strains were applied as starter in a bread making process using whole-grain flour. L. casei developed in sourdoughs by fermenting the existing carbohydrates giving place to an acidification. In this food model system the contribution of L. casei strains expressing phytases to phytate hydrolysis was low, and the phytate degradation was mainly produced by activation of the cereal endogenous phytase as a consequence of the drop in pH. This work shows the capacity of lactobacilli to be modified in order to produce enzymes with relevance in food technology processes. The ability of these strains in reducing the phytate content in fermented food products must be evaluated in further models. PMID:26384212

  3. PEMANFAATAN MILK CLOTTING ENZYME DARI Lactobacillus casei D11 UNTUK PEMBUATAN KEJU MOZZARELLA [Utilization of Milk Clotting Enzyme from Lactobacillus casei D11 for Mozzarella Cheese Making

    Directory of Open Access Journals (Sweden)

    Rohmatussolihat -

    2015-07-01

    Full Text Available Milk Clotting Enzyme (MCE is an active agent for cheese making which may be produced by Lactic acid bacteria (LAB. MCE activity differs according to the LAB strains used. Lactobacillus casei D11 could produced MCE when it is grown in MRS broth medium. In this study, MCE of L. casei D11 with the addition of rennet is used and optimized for the production of mozzarella cheese using Response Surface Method (RSM with Central Composite Design (CCD. The organoleptic properties were determined by hedonics test involving 30 respondents and analyzed statistically which was followed by a Duncan's test. Furthermore, a proximate analysis of mozzarella cheese was conducted. Our results show that the MCE activity produced by L. casei D11 was 8.471 Soxhlet Unit with protease activity of 3.28 U/mL. The ANOVA results showed that the concentration of MCE significantly influence the production of curd. Theoptimum concentration of MCE and rennet for the production of curd suited for the production of mozzarella cheese were 20 and 0.002%, respectively, with a maximum predicted curd yield of 14.996% (g/100 mL milk which is increased by 13.9% as compared to the curd yield before optimization. The statistical analysis on taste, color, flavor, and cheese texture by respondents shows that mozzarella cheese made by a combination of 15% of MCE and 0.00079 and 0.0015% of rennet, were organoleptically superior to the commercial mozzarella used in this experiment. The proximate analysis shows that mozzarella produced has a moisture content of 33.34%(w/w, 3.48% ash, 30.44% fat, 25.12% protein, 7.53% carbohydrate and energy of 404 kkal/100g.

  4. Survival of Lactobacillus rhamnosus probiotic strains in peach jam during storage at different temperatures

    Directory of Open Access Journals (Sweden)

    Cinzia Lucia Randazzo

    2013-12-01

    Full Text Available The survival of six probiotic wild strains of Lactobacillus rhamnosus was compared with that of a type strain during 78 days of storage at 25 and 5 ºC in peach synthetic medium (PSM and commercial peach jam (PJ. Changes in viable cell counts, pH values, sugar content, and colour parameters were monitored. All strains exhibited better performances in PJ than in PSM, showing count values higher than 7 Log cfu g-1 up to 78 days of storage at 5 ºC. Almost all wild strains remained above the critical value of 6 Log cfu g-1 in samples stored at 25 ºC up to 45 days, while the Lb. rhamnosus GG type strain, used as control, was not able to survive later than 15 days. In the synthetic medium used, the strains showed better survival in the samples incubated at 25 ºC, remaining viable above the critical level up to 45 days of storage, except for the strain H12. The probiotic cultures added to jam did not significantly change the colour parameters of the product; however the metabolism of lactobacilli did cause changes in the pH and in the composition of sugars.

  5. Application of response surface methodology to enhancement of biomass production by Lactobacillus rhamnosus E/N

    Directory of Open Access Journals (Sweden)

    Magdalena Polak-Berecka

    2011-12-01

    Full Text Available Response surface methodology (RSM was employed to study the effects of various medium components on biomass production by Lactobacillus rhamnosus E/N. This strain is commonly used in the pharmaceutical and food industries due to its beneficial effect on the human gut and general health. The best medium composition derived from RSM regression was (in g/l glucose 15.44, sodium pyruvate 3.92, meat extract 8.0, potassium phosphate 1.88, sodium acetate 4.7, and ammonium citrate 1.88. With this medium composition biomass production was 23 g/l of dry cell weight after 18 h of cultivation in bioreactor conditions, whereas on MRS the yield of biomass was 21 g/l of dry cell weight. The cost of 1 g of biomass obtained on MRS broth was calculated at the level of 0.44 € whereas on the new optimal medium it was 25% lower. It may be concluded then, that the new medium, being cheaper than the control MRS allows large scale commercial cultivation of the L. rhamnosus strain. This study is of relevance to food industry because the possibility to obtain high yield of bacterial biomass is necessary step in manufacturing of probiotic food.

  6. Characterization of a New Cell Envelope Proteinase PrtP from Lactobacillus rhamnosus CGMCC11055.

    Science.gov (United States)

    Guo, Tingting; Ouyang, Xudong; Xin, Yongping; Wang, Yue; Zhang, Susu; Kong, Jian

    2016-09-21

    Cell envelope proteinases (CEPs) play essential roles in lactic acid bacteria growth in milk and health-promoting properties of fermented dairy products. The genome of Lactobacillus rhamnosus CGMCC11055 possesses two putative CEP genes prtP and prtR2, and the PrtP displays the distinctive domain organization from PrtR2 reported. The PrtP was purified and biochemically characterized. The results showed that the optimal activity occurred at 44 °C, pH 6.5. p-Amidinophenylmethylsulfonyl fluoride obviously inhibited enzymatic activity, suggesting PrtP was a member of serine proteinases. Under the optimal conditions, β-casein was a favorite substrate over αS1- and κ-casein, and 35 oligopeptides were identified in the β-casein hydrolysate, including the phosphoserine peptide and bioactive isoleucine-proline-proline. By analysis of the amino acid sequences of those oligopeptides, proline was the preferred residue at the breakdown site. Therefore, we speculated that PrtP was a new type of CEPs from Lb. rhamnosus. PMID:27585760

  7. The effect of Lactobacillus paracasei subsp. paracasei L. casei W8® on blood levels of triacylglycerol is independent of colonisation

    DEFF Research Database (Denmark)

    Bjerg, A.T.; Sørensen, M.B.; Krych, L.;

    2015-01-01

    Gut microbiota (GM) dysbiosis has been linked to obesity and its metabolic complications such as cardiovascular disease (CVD). The risk of developing CVD increases with elevated concentration of serum triacylglycerol (TAG). In a blinded, randomised two-arm parallel human intervention study we have...... study we analysed faecal samples obtained during the intervention study to investigate whether this effect was related to the ability of L. casei W8 to colonise the human gut after supplementation of L. casei W8 (1010 cfu daily) as determined by qPCR specific for L. paracasei and L. casei (L. casei...

  8. Some aspects of cultivation and utilization of waxy maize (Zea mays L. ssp. ceratina)

    OpenAIRE

    Agnieszka Klimek-Kopyra; Aleksander Szmigiel; Tadeusz Zając; Agnieszka Kidacka

    2012-01-01

    This paper is a review of available literature on Zea mays L.ssp.ceratina. It contains information on the origin, cul- tivation and utilization of waxy maize in the world and can be a contribution to the development of new research on maize cultivation and starch processing technology. Maize, as an old and economically important cereal, played an enormous role in the ancient civilisations of the New World. Among the maize subspecies compared, Z. mays ssp. indurata and Z. mays ssp. indenta...

  9. Biofilms of vaginal Lactobacillus reuteri CRL 1324 and Lactobacillus rhamnosus CRL 1332: kinetics of formation and matrix characterization.

    Science.gov (United States)

    Leccese Terraf, María Cecilia; Juárez Tomás, María Silvina; Rault, Lucie; Le Loir, Yves; Even, Sergine; Nader-Macías, María Elena Fátima

    2016-09-01

    Adhesion and biofilm formation are strain properties that reportedly contribute to the permanence of lactobacilli in the human vagina. The kinetics of biofilm formation and the chemical nature of the biofilm matrix formed by Lactobacillus reuteri CRL (Centro de Referencia para Lactobacilos Culture Collection) 1324 and Lactobacillus rhamnosus CRL 1332, vaginal beneficial strains, were evaluated in this work. Crystal violet-stained microplate assay and techniques of epifluorescence, electron and confocal microscopy were applied. The highest density and complexity of biofilms of both vaginal lactobacilli were observed at 72 h of incubation. Protease, proteinase K, α-chymotrypsin and trypsin treatments efficiently detached L. reuteri CRL 1324 biofilm that was also partially affected by α-amylase. However, L. rhamnosus CRL 1332 biofilm was slightly affected by protease, proteinase K and α-amylase. Confocal microscopy revealed greater amount of polysaccharides in L. rhamnosus CRL 1332 biofilm matrix than in L. reuteri CRL 1324 biofilm matrix. The results indicate that proteins are one of the main components of the L. reuteri CRL 1324 biofilm, while the biofilm matrix of L. rhamnosus CRL 1332 is composed of carbohydrates and proteins. The results obtained support the knowledge, understanding and characterization of two biofilm-forming vaginal Lactobacillus strains. PMID:27146055

  10. Mucosal adhesion properties of the probiotic Lactobacillus rhamnosus GG SpaCBA and SpaFED pilin subunits

    NARCIS (Netherlands)

    Ossowski, von I.; Reunanen, J.; Satokari, R.M.; Vesterlund, S.; Kankainen, M.; Huhtinen, H.; Tynkkynen, S.; Salminen, S.; Vos, de W.M.; Palva, A.

    2010-01-01

    Lactobacillus rhamnosus GG is a well-established Gram-positive probiotic strain, whose health-benefiting properties are in part dependent upon a prolonged residency in the gastrointestinal tract and likely dictated by an adherence to the intestinal mucosa. Previously, we identified two pilus gene cl

  11. Comparison of bioactive components in pressurized and pasteurized longan juices fortified with encapsulated Lactobacillus casei 01

    Science.gov (United States)

    Chaikham, Pittaya; Apichartsrangkoon, Arunee

    2012-06-01

    In this study, longan juice was subjected to a high pressure of 500 MPa for 30 min and compared with a juice pasteurized at 90°C/2 min. Probiotic Lactobacillus casei 01 was fortified into both juices and the shelf life of these products was studied. Their bioactive components such as ascorbic acid, gallic acid and ellagic acid were analyzed by High Performance Liquid Chromatography (HPLC). Total phenolic compounds and 2,2-Diphenyl-1-picrythydrazyl radical-scavenging activity were determined by colorimetric and spectrophotometric methods. It was found that the pressurized longan juice retained higher amounts of bioactive compounds than the pasteurized juice. In terms of storage stability, bioactive compounds in both processed juices decreased according to the increase in storage time. The survivability of probiotic L. casei 01 in both processed juices declined from 9 to 6 log CFU/mL after 4 weeks of storage.

  12. PENGEMBANGAN SSP TEMATIK INTEGRATIF UNTUK MEMBANGUN KARAKTER KEJUJURAN DAN KEPEDULIAN SISWA SD KELAS II

    Directory of Open Access Journals (Sweden)

    Sri Hariyati Qodriyah

    2015-07-01

    Full Text Available Penelitian ini bertujuan untuk menghasilkan perangkat pembelajaran berupa SSP Tematik yang dapat mengembangkan karakter siswa kelas 2 sekolah dasar, meliputi karakter kejujuran dan kepedulian. Penelitian ini merupakan penelitian dan pengembangan yang terdiri dari tujuh tahap, yaitu studi pendahuluan, perencanaan, mengembangkan produk awal, uji coba awal, revisi produk utama, uji coba lapangan, dan revisi terhadap produk operasional. SSP yang dikembangkan dievaluasi oleh seorang ahli materi dan media untuk mengetahui validitasnya. Subjek uji coba berjumlah 90 siswa terdiri dari 23 siswa SD Sonosewu, Kasihan, Bantul, DIY sebagai subjek uji coba terbatas dan 33 siswa untuk kelas control dan 34 siswa untuk kelas eksperimen SD 1 Kadipiro, Kasihan, Bantul, DIY. Hasil penelitian ini berupa SSP yang meliputi: silabus, RPP, LKS, dan lembar penilaian. Hasil evaluasi dari ahli materi dan ahli media untuk menguji tingkat kevalidan SSP menyatakan bahwa SSP yang dikembangkan adalah valid dan berkategori “baik”. Hasil uji coba menunjukkan bahwa SSP yang dikembangkan dinyatakan layak serta memenuhi kriteria praktis dan efektif dalam mengembangkan karakter siswa. Pembelajaran dengan SSP yang dikembangkan mampu mengembangkan karakter kejujuran dan kepedulian. Kata Kunci: SSP, karakter jujur dan peduli   DEVELOPING THEMATIC INTEGRATIVE SSP FOR BUILDING THE CHARACTERS OF HONESTY AND CARE GRADE II STUDENT OF ELEMENTARY SCHOOL Abstract This study aims to produce integrated learning sets in the form of thematic SSP that can develop the characters of honesty and care of grade II students of elementary school. This was a research and development study consisting of seven steps, i.e. preliminary study, planning, preliminary product development, preliminary tryout, main product revision, field tryout, revision of operational product, operational product revision, and revision of the final product. The developed SSP was evaluated by a science subject expert to assess

  13. Xylitol-mediated transient inhibition of ribitol utilization by Lactobacillus casei.

    OpenAIRE

    London, J; Hausman, S

    1982-01-01

    The growth of Lactobacillus casei strain Cl-16 at the expense or ribitol was inhibited if the non-metabolizable substrate xylitol was included in the medium at concentrations of 6 mM or greater. At these concentrations, xylitol, did not competitively inhibit ribitol transport. The cessation of growth was caused by the intracellular accumulation of xylitol-5-phosphate, which occurred because growth on ribitol had gratuitously induced a functional xylitol-specific phosphotransferase system but ...

  14. Preparation and Purification of Xylitol-5-Phosphate from a Cell Extract of Lactobacillus casei Cl-16

    OpenAIRE

    Trahan, L.; Néron, S.; Bareil, M.

    1988-01-01

    A simple procedure which yields pure xylitol-5-phosphate is described. A cell extract of Lactobacillus casei Cl-16 from a 6-liter culture was used to synthesize up to 70 mg of xylitol-5-phosphate overnight from xylitol and phosphoenolpyruvate via a xylitol phosphoenolpyruvate:phosphotransferase system with a 53% yield. Centrifugation, filtration, precipitation as a barium salt, and ion-exchange batch chromatography permitted recovery of nearly 90% of the phosphorylated product synthesized. Th...

  15. Protective effect of Lactobacillus casei on Pseudomonas aeruginosa infection in mice.

    OpenAIRE

    Miake, S; Yokokura, T; Yoshikai, Y; Mutai, M; Nomoto, K.

    1985-01-01

    The protective effect of heat-killed Lactobacillus casei YIT9018 (LC 9018) against Pseudomonas aeruginosa infection in mice was compared with that of Corynebacterium parvum. Survival of mice after intraperitoneal (i.p.) infection with P. aeruginosa was augmented in mice that had been pretreated i.p. with LC 9018 5 days previously. Similar treatment of mice with C. parvum, however, was not effective at all. Moreover, mice became more susceptible to infection with P. aeruginosa after such treat...

  16. Antimicrobial effects of GL13K peptide coatings on S. mutans and L. casei

    Science.gov (United States)

    Schnitt, Rebecca Ann

    Background: Enamel breakdown around orthodontic brackets, so-called "white spot lesions", is the most common complication of orthodontic treatment. White spot lesions are caused by bacteria such as Streptococci and Lactobacilli, whose acidic byproducts cause demineralization of enamel crystals. Aims: The aim of this project was to develop an antimicrobial peptide coating for titanium alloy that is capable of killing acidogenic bacteria, specifically Streptococcus mutans and Lactobacillus casei. The long-term goal is to create an antimicrobial-coated orthodontic bracket with the ability to reduce or prevent the formation of white spot lesions in orthodontic patients thereby improving clinical outcomes. Methods: First, an alkaline etching method with NaOH was established to allow effective coating of titanium discs with GL13K, an antimicrobial peptide derived from human saliva. Coatings were verified by contact angle measures, and treated discs were characterized using scanning electron microscopy. Secondly, GL13K coatings were tested against hydrolytic, proteolytic and mechanical challenges to ensure robust coatings. Third, a series of qualitative and quantitative microbiology experiments were performed to determine the effects of GL13K--L and GL13K--D on S. mutans and L. casei, both in solution and coated on titanium. Results: GL13K-coated discs were stable after two weeks of challenges. GL13K--D was effective at killing S. mutans in vitro at low doses. GL13K--D also demonstrated a bactericidal effect on L. casei, however, in contrast to S. mutans, the effect on L. casei was not statistically significant. Conclusion: GL13K--D is a promising candidate for antimicrobial therapy with possible applications for prevention of white spot lesions in orthodontics.

  17. Antioxidative Activity of Lactobacillus casei subsp. casei SY13 on Ageing Model Mice%干酪乳杆菌干酪亚种 Lactobacillus casei subsp.casei SY13 对衰老模型小鼠的抗氧化作用

    Institute of Scientific and Technical Information of China (English)

    张书文; 吕加平; 孟和毕力格; 刘鹭; 胡鲜宝

    2010-01-01

    [目的]利用一株经体外筛选具有较强抗氧化活性的干酪乳杆菌干酪亚种(Lactobacillus casei subsp.casei)SY13活菌制剂和灭活制剂研究D-半乳糖所致衰老模型小鼠体内抗氧化能力.[方法]采用SY13活菌制剂和灭活制剂饲喂由D-半乳糖连续皮下注射诱导的亚急性衰老模型小鼠,以各组小鼠血清和肝组织匀浆中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力、总抗氧化能力(T-AOC)及脂质过氧化产物丙二醛(MDA)的含量为指标,全面评价其在小鼠体内的抗氧化能力.[结果]Lactobacillus casei subsp.casei SY13活菌制剂高,中剂量组(20、10 mL·kg-1)能降低连续6周注射D-半乳糖诱发的衰老小鼠血清和肝脏组织中的MDA含量,同时提高血清和肝脏组织中GSH-Px活力以及总抗氧化能力(T-AOC),SOD活力变化不明显.[结论]SY13活菌制剂能有效提高亚急性衰老小鼠的抗氧化能力,具有一定延缓衰老的作用.

  18. Metabolism of azo dyes by Lactobacillus casei TISTR 1500 and effects of various factors on decolorization

    OpenAIRE

    Seesuriyachan, Phisit; Takenaka, Shinji; Kuntiya, Ampin; Klayraung, Srikarnjana; Murakami, Shuichiro; Aoki, Kenji

    2007-01-01

    食品破水処理場から分離したLactobacillus casei TISTR 1500 は、アゾ染料を分解することを見いだした。培養条件を検討し、最適分解条件を設定するとともに、アゾ染料の代謝経路を明らかにした。

  19. PHYSICO-CHEMICAL ANALYSIS OF FRESH AND FERMENTED FRUIT JUICES PROBIOTICATED WITH LACTOBACILLUS CASEI

    OpenAIRE

    Bathal Vijaya Kumar; Mannepula Sreedharamurthy; Obulam Vijaya Sarathi Reddy

    2013-01-01

    The objective of this study was to find out the suitability of different fruit juices for probiotication by using Lactobacillus casei. Phyto-chemical analysis of different fruit juices (mango, sapota, grape and cantaloupe) were carried out using the standard methods. Carbohydrates, flavoniods, tannins, glycosides were present and alkaloids and saponins were absent in all the above fruit juices. Further analysis by TLC and DPPH methods indicated good antioxidant activity in all the fruit juice...

  20. Optimization of probiotic lactobacillus casei ATCC 334 production using date powder as carbon source

    OpenAIRE

    Shahravy A.; Tabandeh F.; Bambai B.; Zamanizadeh H.R.; Mizani M.

    2012-01-01

    This study was conducted to optimize culture conditions for economic production of a probiotic bacterium, Lactobacillus casei ATCC 334, in which palm date powder was applied for the first time as a low-cost main carbon source. The effect of eleven factors on bacterial growth was investigated using the Taguchi experimental design, and three factors including palm date powder, tryptone and agitation rate were found to be the most significant parameters. The optimum conditions including da...

  1. Lactobacillus rhamnosus accelerates zebrafish backbone calcification and gonadal differentiation through effects on the GnRH and IGF systems.

    Directory of Open Access Journals (Sweden)

    Matteo A Avella

    Full Text Available Endogenous microbiota play essential roles in the host's immune system, physiology, reproduction and nutrient metabolism. We hypothesized that a continuous administration of an exogenous probiotic might also influence the host's development. Thus, we treated zebrafish from birth to sexual maturation (2-months treatment with Lactobacillus rhamnosus, a probiotic species intended for human use. We monitored for the presence of L. rhamnosus during the entire treatment. Zebrafish at 6 days post fertilization (dpf exhibited elevated gene expression levels for Insulin-like growth factors -I and -II, Peroxisome proliferator activated receptors -α and -β, VDR-α and RAR-γ when compared to untreated-10 days old zebrafish. Using a gonadotropin-releasing hormone 3 GFP transgenic zebrafish (GnRH3-GFP, higher GnRH3 expression was found at 6, 8 and 10 dpf upon L. rhamnosus treatment. The same larvae exhibited earlier backbone calcification and gonad maturation. Noteworthy in the gonad development was the presence of first testes differentiation at 3 weeks post fertilization in the treated zebrafish population -which normally occurs at 8 weeks- and a dramatic sex ratio modulation (93% females, 7% males in control vs. 55% females, 45% males in the treated group. We infer that administration of L. rhamnosus stimulated the IGF system, leading to a faster backbone calcification. Moreover we hypothesize a role for administration of L. rhamnosus on GnRH3 modulation during early larval development, which in turn affects gonadal development and sex differentiation. These findings suggest a significant role of the microbiota composition on the host organism development profile and open new perspectives in the study of probiotics usage and application.

  2. Lactobacillus strains belonging to Casei group display various adherence to enterocytes and mucus

    Directory of Open Access Journals (Sweden)

    Corinna Markowicz

    2015-09-01

    Full Text Available Background. The ability of lactobacilli to adhere to the surface of the intestine is an important functional characteristic which can largely determine the effective colonization of the intestinal tract by probiotics. The following study compares the adhesion efficiency of the twenty strains of Lactobacillus genus belonging to Casei group to the Caco-2 cells and gastrointestinal mucus. Material and methods. Twenty isolates of lactobacilli belonging to Casei group were tested. The ability of bacterial cells to adhere to mucus was examined using adhesion assay to gastrointestinal mucus. Obtained results were compared with adhesion efficiency to Caco-2 cells. Phylogenetic relationship between isolates was analysed by rep-PCR. Results. The results showed large differences in adhesion efficiency between strains, as well as differences in the efficiency of adhesion to the intestinal epithelial cells and mucus. Group similarity highlighted by a rep-PCR technique does not correspond with groups of similarity in terms of the characteristics of the ability to adhere to mucus or the epithelial cells of intestinal tract. Conclusions. Strains having a high adhesion efficiency to enterocytes do not always show a high adhesion efficiency to the mucus. This may indicate the presence of different and multiple factors responsible for adhesion efficiency of Lactobacillus group Casei strains to epithelial cells and mucus.

  3. Performance in nondairy drinks of probiotic L. casei strains usually employed in dairy products.

    Science.gov (United States)

    Céspedes, Mario; Cárdenas, Pamela; Staffolani, Martín; Ciappini, María C; Vinderola, Gabriel

    2013-05-01

    The increase in vegetarianism as dietary habit and the increased allergy episodes against dairy proteins fuel the demand for probiotics in nondairy products. Lactose intolerance and the cholesterol content of dairy products can also be considered two additional reasons why some consumers are looking for probiotics in other foods. We aimed at determining cell viability in nondairy drinks and resistance to simulated gastric digestion of commercial probiotic lactobacilli commonly used in dairy products. Lactobacillus casei LC-01 and L. casei BGP 93 were added to different commercial nondairy drinks and viability and resistance to simulated gastric digestion (pH 2.5, 90 min, 37 °C) were monitored along storage (5 and 20 °C). For both strains, at least one nondairy drink was found to offer cell counts around 7 log orders until the end of the storage period. Changes in resistance to simulated gastric digestion were observed as well. Commercial probiotic cultures of L. casei can be added to commercial fruit juices after a carefull selection of the product that warrants cell viability. The resistance to simulated gastric digestion is an easy-to-apply in vitro tool that may contribute to product characterization and may help in the choice of the food matrix when no changes in cell viability are observed along storage. Sensorial evaluation is mandatory before marketing since the product type and storage conditions might influence the sensorial properties of the product due to the possibility of growth and lactic acid production by probiotic bacteria. PMID:23527588

  4. Expression of Human Chorionic Gonadotropin β (hCGβ) in Lactobacillus Casei

    Institute of Scientific and Technical Information of China (English)

    Hong-mei WANG; Hong JIANG; Zheng-hong YUAN; Da-jin LI

    2003-01-01

    Objective To construct a recombinant lactobacillus (Lb.) strain excreting the human chorionic gonadotropin beta-subunit, hCGβMethods The hCGβ cDNA was ligated to the signal peptide sequence of S-layer protein from Lb. brevis and then cloned into down-stream of lactose-inducible promoter of an integrative plasmid, pIlac. After electroporation into Lb. casei CECT5276, PCR using the genomic DNA of the recombinant lactobacillus as template was performed to confirm whether the hCGβ gene had been integrated into the genome. Radioimmunoassay (RIA) was used to determine the level of hCGβ in the supernatant and the cell lysate. Results The hCGβ was integrated into the genome of Lb. casei CECT5276. The highest concentration of hCGβ in the culture supernatant amounted to 440 mIU/mL 21 h after lactose induction. About 2/3 of the objective proteins were excreted into the supernatant.Conclusion We have obtained stable and efficient hCGβ excretion in Lb. casei, which was inducible by lactose.

  5. 益生菌Lactobacillus casei Zhang对凝固型发酵乳感官品质的影响%Effects of Probiotic Lactobacillus casei Zhang on Sensory Properties of Set Fermented Milk

    Institute of Scientific and Technical Information of China (English)

    王记成; 郭壮; 其木格苏都; 张和平; 孟和毕力格

    2013-01-01

    [目的]评价益生菌Lactobacillus casei Zhang(L.casei Zhang)对凝固型发酵乳感官品质的影响.[方法]将Streptococcus thermophilus(S.thermophilus)与益生菌L.caseiZhang复配发酵,发酵结束(pH4.5)于4℃冷藏1d或28d,并对发酵乳进行感官鉴评试验.[结果]益生菌L.casei Zhang与S.thermophilus 复配发酵可赋予产品更好的细腻性、更为浓郁的奶油味和乙醛风味,从而提升产品的风味总体得分、气味总体得分及产品总得分.4℃贮藏7d时益生菌发酵乳具有最好的气味和风味感官特征,而贮藏14d时具有最好的表观和质地感官特征.[结论]益生菌L.casei Zhang可提高发酵乳制品的感官品质,同时贮藏时间对发酵乳感官特性产生影响.%[Objective] The objective of this study is to evaluate the effects of the probiotic Lactobacillus casei Zhang on the sensory properties of set fermented milk.[Method] Probiotic L.casei Zhang was inoculated together with the S.thermophilus strains into pasteurized milk for fermentation.The fermentation was ended when the pH value of fermented milk reached 4.5.Then,sensory properties of the products were determined after storage at 4℃ for 1 d or 28 d.[Result] Sensory analysis showed that the score of glossiness,acetaldehyde,buttery,acceptance of flavor and odor attributes of the fermented milks produced by probiotic L.casei Zhang and S.thermophilus were higher than those produced by single starter culture.Meanwhile,the acceptance of flavor and odor attributes of all of the fermented milks decreased after 7 days,and the highest sensory score of the acceptance of texture and appearance attributes were observed after 14 days of refrigerated storage.[Conclusion] Probiotic L.casei Zhang could improve the overall sensory quality of fermented milk,meanwhile the storage time had effects on some sensory characteristics of fermented milk.

  6. 荧光定量PCR法检测益生菌饮料中Lactobacillus casei Zhang和Bifidobacterium lactis V9%Lactobacillus casei Zhang Bifidobacterium lactis V9 Method of fluorescent quantitative PCR for detection of Lactobacillus casei Zhang and Bifidobacterium lactis V9

    Institute of Scientific and Technical Information of China (English)

    其木格苏都; 王记成; 张家超; 张和平

    2011-01-01

    益生菌活菌数是益生菌产品最重要的指标,而检测益生菌方法的准确性和科学性则至关重要.本文采用荧光定量PCR法同时定量检测益生菌饮料中Lactobacillus casei Zhang(L.casei Zhang)和Bifidobacterium lactis V9(B.lactis V9)的活菌数,并与平板菌落计数法进行比较.结果表明,荧光定量PCR法测得L.casei Zhang活菌数与平板菌落计数法测得活菌数差异不显著;而采用荧光定量PCR法测得B.lactis V9活菌数显著高于平板菌落计数法.荧光定量PCR法灵敏、特异、简便快速,可定量检测并真实反应L.casei Zhang和B.lactis V9的活菌数.%The viable count of probiotic is considered as the most important index and the accurate and scientific assay of probiotic viable number is essential to probiotic products. In the present study, the fluorescent quantitative PCR method and plate colony counting method were used and compared for detection of bacterial viable numbers of Lactobadllus casei Zhang and Bifidobacterium lactis V9 in probiotic fermented beverage. The result showed that there was no significant difference of L. Casei Zhang number between two methods, whereas the numbers of B. Lactis V9 detected by two methods were significant difference. It is suggested that fluorescent quantitative PCR method appear to be highly accurate, specific, fast and reliable for quantification of L. Casei Zhang and B. Lactis V9.

  7. Clinical trial: multispecies probiotic supplementation alleviates the symptoms of IBS and stabilises intestinal microbiota

    NARCIS (Netherlands)

    Kajander, K.; Myllyluoma, E.; Rajlic-Stojanovic, M.; Kyronpalo, S.S.; Rasmussen, M.; Jarvenpaa, S.S.; Zoetendal, E.G.; Vos, de W.M.; Vapaatalo, H.; Korpela, R.

    2008-01-01

    Aim To investigate the effects of multispecies probiotic supplementation (Lactobacillus rhamnosus GG, L. rhamnosus Lc705, Propionibacterium freudenreichii ssp. shermanii JS and Bifidobacterium animalis ssp. lactis Bb12) on abdominal symptoms, quality of life, intestinal microbiota and inflammatory m

  8. Identification and functional characterization of AclB, a novel cell-separating enzyme from Lactobacillus casei.

    Science.gov (United States)

    Xu, Yi; Wang, Ting; Kong, Jian; Wang, Hui-Li

    2015-06-16

    Autolysis of nonstarter lactic acid bacteria (NSLAB) was favorable for the development of flavor compounds during cheese manufacture. Among these bacteria, Lb. casei was regarded as the most important microbiota involved in cheese processes. In this study, a novel autolysin named AclB was identified in the genome of Lb. casei BL23 and its modular structure was predicted through bioinformatic approaches. Subsequently, its transcription profile in the exponential phase, hydrolytic activities against cell walls, enzymatic properties under different conditions, physiological function via gene inactivation and upregulation assays, as well as potential applications to NSLAB's autolysis were fully investigated. According to the results, AclB was recognized as a species-specific cell-separating enzyme, responsible for cell separation after cell division in Lb. casei BL23. The purified AclB showed considerable hydrolyzing activities towards cell walls, indicating its enzymatic nature as peptidoglycan hydrolase, or autolysin. The highest activity of AclB was determined at pH5.0 and 37°C, and the expression vector constructed based on AclB was shown to facilitate the controlled lysis of Lb. casei BL23 hosts. In summary, this study provided insight into the enzymatic properties of a novel autolysin involved in cell separation of Lb. casei BL23, which is promising to accelerate cheese ripening and improve cheese quality. PMID:25797034

  9. Lactobacillus casei stimulates phase-II detoxification system and rescues malathion-induced physiological impairments in Caenorhabditis elegans.

    Science.gov (United States)

    Kamaladevi, Arumugam; Ganguli, Abhijit; Balamurugan, Krishnaswamy

    2016-01-01

    Malathion, an organophosphorus insecticide, is renowned for its inhibitory action on acetylcholinesterase (AChE) enzyme that eventually leads to widespread disturbance in the normal physiological and behavioral activities of any organism. Lactic acid bacteria (LAB) are still an underexploited and inexhaustible source of significant pharmaceutical thrust. In the present study, Caenorhabditis elegans was employed to identify and characterize the indigenous LAB isolated from different traditional food against malathion-induced toxicity. The results demonstrated that malathion at its LD50 concentration decreased various C. elegans physiological parameters such as survival, feeding, and locomotion. Among the screened isolates, L. casei exhibited an excellent protective efficacy against malathion-induced toxicity by increasing the level of AChE and thereby rescued all physiological parameters of C. elegans. In addition, short-term exposure and food choice assay divulged that L. casei could serve as a better food to protect C. elegans from noxious environment. The expression analysis unveiled that L. casei gavage upregulated the phase-II detoxification enzymes coding genes metallothioneins (mtl-1 and mtl-2) and glutathione-S-transferase (gst-8) and thereby eliminated malathion from the host system. Furthermore, the upregulation of ace-3 along with down-regulation of cyp35a in the nematodes supplemented with L. casei could be attributed to attenuate the malathion-induced physiological defects in C. elegans. Thus, the present study reports that an indigenous LAB-L. casei could serve as a promising protective agent against the harmful effects of pesticide. PMID:26297616

  10. Isolation, Identification and Partial Characterization of a Lactobacillus casei Strain with Bile Salt Hydrolase Activity from Pulque.

    Science.gov (United States)

    González-Vázquez, R; Azaola-Espinosa, A; Mayorga-Reyes, L; Reyes-Nava, L A; Shah, N P; Rivera-Espinoza, Y

    2015-12-01

    The aim of this study was to isolate, from pulque, Lactobacillus spp. capable of survival in simulated gastrointestinal stress conditions. Nine Gram-positive rods were isolated; however, only one strain (J57) shared identity with Lactobacillus and was registered as Lactobacillus casei J57 (GenBank accession: JN182264). The other strains were identified as Bacillus spp. The most significant observation during the test of tolerance to simulated gastrointestinal conditions (acidity, gastric juice and bile salts) was that L. casei J57 showed a rapid decrease (p ≤ 0.05) in the viable population at 0 h. Bile salts were the stress condition that most affected its survival, from which deoxycholic acid and the mix of bile salts (oxgall) were the most toxic. L. casei J57 showed bile salt hydrolase activity over primary and secondary bile salts as follows: 44.91, 671.72, 45.27 and 61.57 U/mg to glycocholate, taurocholate, glycodeoxycholate and taurodeoxycholate. In contrast, the control strain (L. casei Shirota) only showed activity over tauroconjugates. These results suggest that L. casei J57 shows potential for probiotic applications.

  11. Lactobacillus casei stimulates phase-II detoxification system and rescues malathion-induced physiological impairments in Caenorhabditis elegans.

    Science.gov (United States)

    Kamaladevi, Arumugam; Ganguli, Abhijit; Balamurugan, Krishnaswamy

    2016-01-01

    Malathion, an organophosphorus insecticide, is renowned for its inhibitory action on acetylcholinesterase (AChE) enzyme that eventually leads to widespread disturbance in the normal physiological and behavioral activities of any organism. Lactic acid bacteria (LAB) are still an underexploited and inexhaustible source of significant pharmaceutical thrust. In the present study, Caenorhabditis elegans was employed to identify and characterize the indigenous LAB isolated from different traditional food against malathion-induced toxicity. The results demonstrated that malathion at its LD50 concentration decreased various C. elegans physiological parameters such as survival, feeding, and locomotion. Among the screened isolates, L. casei exhibited an excellent protective efficacy against malathion-induced toxicity by increasing the level of AChE and thereby rescued all physiological parameters of C. elegans. In addition, short-term exposure and food choice assay divulged that L. casei could serve as a better food to protect C. elegans from noxious environment. The expression analysis unveiled that L. casei gavage upregulated the phase-II detoxification enzymes coding genes metallothioneins (mtl-1 and mtl-2) and glutathione-S-transferase (gst-8) and thereby eliminated malathion from the host system. Furthermore, the upregulation of ace-3 along with down-regulation of cyp35a in the nematodes supplemented with L. casei could be attributed to attenuate the malathion-induced physiological defects in C. elegans. Thus, the present study reports that an indigenous LAB-L. casei could serve as a promising protective agent against the harmful effects of pesticide.

  12. Applications of Lactobacillus rhamnosus Spent Culture Supernatant in Cosmetic Antioxidation, Whitening and Moisture Retention Applications

    Directory of Open Access Journals (Sweden)

    Cheng-Chih Tsai

    2013-11-01

    Full Text Available This study was aimed at investigating the antioxidant, whitening, and moisture-retention properties of Lactobacillus rhamnosus spent culture supernatant (Lr-SCS in cosmetic applications. Results reveal that Lr-SCS effectively and gradually scavenges 1,1-diphenyl-2-picrylhydrazyl as well as 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid radical cations, and increases reducing power in a dose-dependent manner. Additionally, Lr-SCS can also suppress tyrosinase activity in vitro and effectively promote moisture retention. Heat treatment at 100 °C for 30 min does not influence the functions of Lr-SCS. We conclude that Lr-SCS can be used effectively in skin care cosmetics.

  13. The effect of consumption of milk fermented by Lactobacillus casei strain Shirota on the intestinal microflora and immune parameters in humans

    NARCIS (Netherlands)

    Spanhaak, S.; Havenaar, R.; Schaafsma, G.

    1998-01-01

    Objective: To determine the effect of consumption of milk fermented by Lactobacillus casei strain Shirota (L. casei Shirota) on the composition and metabolic activities of the intestinal microflora, and immune parameters in humans. Subjects: Twenty healthy male subjects aged 40-65 years were selecte

  14. Influence of manufacturing processes on cell surface properties of probiotic strain Lactobacillus rhamnosus Lcr35®.

    Science.gov (United States)

    Nivoliez, Adrien; Veisseire, Philippe; Alaterre, Elina; Dausset, Caroline; Baptiste, Fabrice; Camarès, Olivier; Paquet-Gachinat, Marylise; Bonnet, Muriel; Forestier, Christiane; Bornes, Stéphanie

    2015-01-01

    The influence of the industrial process on the properties of probiotics, administered as complex manufactured products, has been poorly investigated. In the present study, we comparatively assessed the cell wall characteristics of the probiotic strain Lactobacillus rhamnosus Lcr35® together with three of its commercial formulations with intestinal applications. Putative secreted and transmembrane-protein-encoding genes were initially searched in silico in the genome of L. rhamnosus Lcr35®. A total of 369 candidate genes were identified which expressions were followed using a custom Lactobacillus DNA chip. Among them, 60 or 67 genes had their expression either upregulated or downregulated in the Lcr Restituo® packet or capsule formulations, compared to the native Lcr35® strain. Moreover, our data showed that the probiotic formulations (Lcr Lenio®, Lcr restituo® capsule and packet) showed a better capacity to adhere to intestinal epithelial Caco-2 cells than the native Lcr35® strain. Microbial (MATS) tests showed that the probiotic was an electron donor and that they were more hydrophilic than the native strain. The enhanced adhesion capacity of the active pharmaceutical ingredients (APIs) to epithelial Caco-2 cells and their antipathogen effect could be due to this greater surface hydrophilic character. These findings suggest that the manufacturing process influences the protein composition and the chemical properties of the cell wall. It is therefore likely that the antipathogen effect of the formulation is modulated by the industrial process. Screening of the manufactured products' properties would therefore represent an essential step in evaluating the effects of probiotic strains. PMID:25280746

  15. Viability of Lactobacillus casei in chocolate flan and its survival to simulated gastrointestinal conditionsViabilidade de Lactobacillus casei em flan de chocolate e sua sobrevivência em condições gastrintestinais simuladas

    Directory of Open Access Journals (Sweden)

    Lina Casale Aragon-Alegro

    2012-02-01

    Full Text Available The aim of this work was to verify the viability of Lactobacillus casei in chocolate flan as well as its survival under simulated human gastrointestinal conditions. After 1 and 15 days of manufacture, the dessert was evaluated for L. casei population and subsequently submitted to acidic solution for 30, 60, 90 and 120 min and to bile salts solution for 150 min. After each time, L. casei population was evaluated. The results were evaluated by variance analysis (ANOVA and Tukey’s test. The population of L. casei in the flan was above 109 CFU/g during the shelf life, whose value is above the limit established for a probiotic food by the Brazilian legislation. L. casei was relatively resistant to the simulated gastrointestinal conditions tested in this study, once the population reduced during the in vitro tests but remained above 106 CFU/g. Chocolate flan showed to be an excellent food for addition of probiotic microorganisms, especially L. casei, once it was observed high populations in the product even after in vitro resistance tests simulating the human digestive process.Neste trabalho, verificou-se a viabilidade de Lactobacillus casei em flan de chocolate, além de sua sobrevivência em condições que simulam o trato gastrointestinal humano. Após 1 e 15 dias da produção, a população de L. casei presente na sobremesa foi avaliada, e o flan foi submetido, em seguida, a uma solução ácida, durante 30, 60, 90 e 120 minutos, e a uma solução de sais biliares, por um período de 150 minutos. Após cada um desses tempos, a população de L. casei foi observada. Os resultados foram avaliados por meio de análise de variância (ANOVA e teste de Tukey. A população de L. casei no flan foi maior que 109 UFC/g durante a vida de prateleira do produto, valor este, maior que o estabelecido para um alimento probiótico pela legislação brasileira. L. casei mostrou-se relativamente resistente às condições simuladas do trato gastrointestinal

  16. 益生菌Lactobacillus casei Zhang冻干粉对小鼠急性毒性的研究%Studies on Acute Toxicity of Lactobacillus casei Zhang Freeze-dried Powder in Mice

    Institute of Scientific and Technical Information of China (English)

    孙洁宇; 高鹏飞; 麻士卫; 杜瑞亭; 王明芳; 邓承远; 高杰; 张和平

    2009-01-01

    通过研究益生菌Lactobacillus casei Zhang冻干粉对小鼠急性毒性作用,为该菌株的安全应用提供依据.连续14 d对小鼠灌胃不同剂量的L. casei Zhang冻干粉,各剂量组小鼠的一般体征、肝脏功能、脏器指数以及肝、肾、脾形态学的观察结果与对照组相比无显著差异(P>0.05).急性毒性试验及相关指标检测结果显示L. casei Zhang冻干粉无毒副作用.

  17. 益生菌Lactobacillus casei Zhang 对酸奶风味、质地及感官特性的影响%Influence of probiotic Lactobacillus casei Zhang on aroma generation, texture and sensory characteristics of yogurt

    Institute of Scientific and Technical Information of China (English)

    郭壮; 王记成; 闫丽雅; 张轩; 刘小鸣; 陈卫; 张和平

    2009-01-01

    将益生菌Lactobacillus casei Zhang(Lb. casei Zhang)以1.0×107g-1的添加量与商业酸奶发酵荆YC-X11共同接种进行发酵乳制备.发酵结束(pH=4.5)于4℃冷藏24 h后,分别测定发酵乳样品的酸度、黏度、脱水收缩性、挥发性风味物质、L.delbrueckii subsp.Bulgaricus、S.thermophilus和Lb.casei Zhang活菌数及对其进行感官鉴评.结果表明.Lb.casei Zhang对发酵乳样品的酸度、黏度、脱水收缩性、S.ther-mophilus活菌数无影响(P>0.05),可促进L.delbrueckii subsp.Bulgaricus生长(P<0.05),并使酸奶样品中挥发性风味物质总的质量分数提高17.1%.从而总体上提高发酵乳的感官品质.同时Lb.casei Zhang在发酵乳中具有良好的稳定性.因而益生菌Lb.casei Zhang与商业酸奶发酵剂YC-X11复配进行发酵乳生产具有极大的可行性.

  18. Nonindependent domestication of the two rice subspecies, Oryza sativa ssp. indica and ssp. japonica, demonstrated by multilocus microsatellites.

    Science.gov (United States)

    Gao, Li-Zhi; Innan, Hideki

    2008-06-01

    The origins of the Asian cultivated rice Oryza sativa from its wild ancestor O. rufipogon have been debated for decades. The question mainly concerns whether it originated monophyletically or polyphyletically. To shed light on the origins and demographic history of rice domestication, we genotyped a total of 92 individual plants from the two O. sativa subspecies and O. rufipogon for 60 microsatellites. An approximate Bayesian method was applied to estimate demographic parameters for O. rufipogon vs. O. sativa ssp. indica and O. rufipogon vs. O. sativa ssp. japonica. We showed that the japonica subspecies suffered a more severe bottleneck than the indica subspecies and thus a greater loss of genetic variation during its domestication. Across microsatellite loci there is a significant positive correlation in the reduction of genetic diversity between the two subspecies. The results suggest that completely independent domestication of indica and japonica subspecies may not explain our data and that there is at least partial sharing of their ancestral populations and/or recent gene flow between them. PMID:18505887

  19. The Impact of Lactobacillus casei on the Composition of the Cecal Microbiota and Innate Immune System Is Strain Specific.

    Science.gov (United States)

    Aktas, Busra; De Wolfe, Travis J; Safdar, Nasia; Darien, Benjamin J; Steele, James L

    2016-01-01

    The probiotic function to impact human health is thought to be related to their ability to alter the composition of the gut microbiota and modulate the human innate immune system. The ability to function as a probiotic is believed to be strain specific. Strains of Lactobacillus casei are commonly utilized as probiotics that when consumed alter the composition of the gut microbiota and modulate the host immune response. L. casei strains are known to differ significantly in gene content. The objective of this study was to investigate seven different L. casei strains for their ability to alter the murine gut microbiota and modulate the murine immune system. C57BL/6 mice were fed L. casei strains at a dose of 108 CFU/day/mouse for seven days and sacrificed 3.5h after the last administration. The cecal content and the ileum tissue were collected for microbiota analysis and immune profiling, respectively. While 5 of the L. casei strains altered the gut microbiota in a strain specific manner, two of the strains did not alter the overall cecal microbiota composition. The observed changes cluster into three groups containing between 1 and 2 strains. Two strains that did not affect the gut microbiota composition cluster together with the control in their impact on pattern recognition receptors (PRRs) expression, suggesting that the ability to alter the cecal microbiota correlates with the ability to alter PRR expression. They also cluster together in their impact on the expression of intestinal antimicrobial peptides (AMPs). This result suggests that a relationship exists between the capability of a L. casei strains to alter the composition of the gut microbiota, PRR regulation, and AMP regulation. PMID:27244133

  20. A phase trial of the oral Lactobacillus casei vaccine polarizes Th2 cell immunity against transmissible gastroenteritis coronavirus infection.

    Science.gov (United States)

    Jiang, Xinpeng; Hou, Xingyu; Tang, Lijie; Jiang, Yanping; Ma, Guangpeng; Li, Yijing

    2016-09-01

    Transmissible gastroenteritis coronavirus (TGEV) is a member of the genus Coronavirus, family Coronaviridae, order Nidovirales. TGEV is an enteropathogenic coronavirus that causes highly fatal acute diarrhoea in newborn pigs. An oral Lactobacillus casei (L. casei) vaccine against anti-transmissible gastroenteritis virus developed in our laboratory was used to study mucosal immune responses. In this L. casei vaccine, repetitive peptides expressed by L. casei (specifically the MDP and tuftsin fusion protein (MT)) were repeated 20 times and the D antigenic site of the TGEV spike (S) protein was repeated 6 times. Immunization with recombinant Lactobacillus is crucial for investigations of the effect of immunization, such as the first immunization time and dose. The first immunization is more important than the last immunization in the series. The recombinant Lactobacillus elicited specific systemic and mucosal immune responses. Recombinant L. casei had a strong potentiating effect on the cellular immunity induced by the oral L. casei vaccine. However, during TGEV infection, the systemic and local immune responses switched from Th1 to Th2-based immune responses. The systemic humoral immune response was stronger than the cellular immune response after TGEV infection. We found that the recombinant Lactobacillus stimulated IL-17 expression in both the systemic and mucosal immune responses against TGEV infection. Furthermore, the Lactobacillus vaccine stimulated an anti-TGEV infection Th17 pathway. The histopathological examination showed tremendous potential for recombinant Lactobacillus to enable rapid and effective treatment for TGEV with an intestinal tropism in piglets. The TGEV immune protection was primarily dependent on mucosal immunity. PMID:27020282

  1. Determination of Critical Point of pO2 Level in the Production of Lactic Acid by Lactobacillus rhamnosus

    Science.gov (United States)

    Mel, Maizirwan; Karim, Mohamed Ismail Abdul; Salleh, Mohamad Ramlan Mohamed; Abdullah, Rohane

    The study was conducted to determine the critical point of pO2 level in the production of lactic acid by Lactobacillus rhamnosus. The fermentation process was successfully carried out in laboratory scale fermenter/bioreactor using different pO2 level (the main parameter that significantly affects the growth of L. rhamnosus and lactic acid production) together with two other parameters; the agitation rate and pH. From the result, it was observed that the best production of lactic acid with the concentration of 16.85 g L-1 or 1.68% production yield has been obtained at the operating parameters of 5% pO2 level, agitation speed of 100 rpm and sample pH 6. The critical point of pO2 was found to be between 5 and 10%.

  2. Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films

    OpenAIRE

    Soukoulis, Christos; Singh, Poonam; MacNaughtan, William; Parmenter, Chistopher; Fisk, Ian D.

    2016-01-01

    Probiotic incorporation in edible films and coatings has been shown recently to be an efficient strategy for the delivery of probiotics in foods. In the present work, the impact of the compositional, physicochemical and structural properties of binary starch-protein edible films on Lactobacillus rhamnosus GG viability and stability was evaluated. Native rice and corn starch, as well as bovine skin gelatine, sodium caseinate and soy protein concentrate were used for the fabrication of the prob...

  3. Epithelial Adhesion Mediated by Pilin SpaC Is Required for Lactobacillus rhamnosus GG-Induced Cellular Responses

    OpenAIRE

    Ardita, Courtney S.; Mercante, Jeffrey W; Kwon, Young Man; Luo, Liping; Crawford, Madelyn E.; Powell, Domonica N.; Jones, Rheinallt M.; Neish, Andrew S.

    2014-01-01

    Lactobacillus rhamnosus GG is a widely used probiotic, and the strain's salutary effects on the intestine have been extensively documented. We previously reported that strain GG can modulate inflammatory signaling, as well as epithelial migration and proliferation, by activating NADPH oxidase 1-catalyzed generation of reactive oxygen species (ROS). However, how strain GG induces these responses is unknown. Here, we report that strain GG's probiotic benefits are dependent on the bacterial-epit...

  4. Production of dry Lactobacillus rhamnosus GG preparations by spray drying and lyophilization in aqueous two-phase systems

    OpenAIRE

    Katarzyna Leja; Radosław Dembczyński; Wojciech Białas; Tomasz Jankowski

    2009-01-01

    Background. Drying is the oldest method of food preservation. It works by removing water from the food, which prevents the growth of microorganisms and decay. Moreover, spray or freeze drying is also used for the preservation of probiotic cultures. The aim of this study was to compare a survival rate of probiotic bacteria Lactobacillus rhamnosus during spray and freeze drying in ATPS. These results were also compared with survival rate of cells dried under the same conditions but suspended on...

  5. Ingeniería metabólica y desarrollos fermentativos en Lactobacillus casei.

    OpenAIRE

    Pérez Martínez, Gaspar

    2005-01-01

    Con el fin de diseñar procedimientos para el aprovechamiento biotecnológico de la lactosa, el principal contaminante del lactosuero, nuestro laboratorio ha desarrollado una herramienta para la inserción de genes metabólicos, por recombinación, en el operón de la lactosa de L. casei. Las cepas resultantes presentan una inserción “limpia”, es decir que no poseen genes de resistencia a antibióticos (grado alimentario) y, además, expresan los genes de interés de forma coordinada con los genes de ...

  6. Batch production of L(+) lactic acid from whey by Lactobacillus casei (NRRL B-441)

    OpenAIRE

    Büyükkileci, Ali Oğuz; Harsa, Hayriye Şebnem

    2004-01-01

    The effects of temperature, pH, and medium composition on lactic acid production by Lactobacillus casei were investigated. The highest lactic acid productivity values were obtained at 37 °C and pH 5.5. The productivity was 1.87 g dm-3 h-1 at 37 °C in shake flasks. In the fermenter, a productivity of 3.97 g dm-3 h-1 was obtained at pH 5.5. The most appropriate yeast extract concentration was 5.0 g dm-3. Whey yielded a higher productivity value than the analytical lactose and glucose. Initial w...

  7. Survival of the probiotic bacteria Lactobacillus rhamnosus in seawater and its bioencapsulation in the brine shrimp Artemia

    Directory of Open Access Journals (Sweden)

    Claudia Ofelio

    2014-06-01

    The results obtained in the first test showed that the L. rhamnosus was able to survive in seawater during the whole experiment (30h, maintaining densities of 10e7 CFU/ml during the first 6h although decreasing progressively afterwards (10e3 CFU/ml at 30h. This allows adequate levels at sufficient time for Artemia to incorporate the probiotic. In fact, bioencapsulation test demonstrated that Artemia metanauplii were able to bioencapsulate the probiotic, reaching the highest concentration in Artemia after 30 min of bioencapsulation (10e4 CFU/Artemia. A slight further decrease (10e3 CFU/Artemia was observed after 24h. Interestingly, L. rhamnosus reduced in 1Log total Vibrionaceae bacteria in Artemia during the 3 first hours. Therefore, 3 hours was the time established for the bioencapsulation protocol and further studies are in progress to determine the ability of Artemia metanauplii to maintain bioencapsulated L. rhamnosus once transferred to rearing tanks. Also, the capability of the probiotic to inhibit potential pathogenic or opportunistic bacteria will be assessed.

  8. A validity study of the SSP-School Inclusion Questionnaire

    Directory of Open Access Journals (Sweden)

    DIANA DĂMEAN

    2012-12-01

    Full Text Available The paper is based on the results of a research that aimed to identify the factors influencing school inclusion among disadvantagedstudents. The main outcome of the research was the development of the SSP-School Inclusion Questionnaire, intended to: (1measure school inclusion levels among students; (2 identify students with significantly lower levels of school inclusion, whoneed to be included in intervention programs; and (3 measure the impact of intervention programs using subjective indicators(beneficiaries’ perception of school and teachers, feelings of safety in school, school performance. The questionnaire was completedby 480 vocational school students aged 15-19 from a large developed urban area (Cluj-Napoca in Romania. The purpose of theresearch was to test the validity and reliability of the instrument and to propose it to professionals working in education (schoolpsychologists, school counsellors etc. for efficient data collection and for measuring the impact of educational intervention.

  9. Phytochemical analysis of the flower extracts of Rhododendron arboreum Sm. ssp. nilagiricum (Zenker) Tagg

    Institute of Scientific and Technical Information of China (English)

    Kiruba S; Mahesh M; Nisha SR; Miller Paul Z; Jeeva S

    2011-01-01

    Objective: To evaluate the preliminary phytochemical screening of the flower extracts of Rhododendron arboreum (R. arboreum) Sm. ssp. nilagiricum (Zenker) Tagg. Methods: The preliminary phytochemical screening was performed by the standard methods as described by Harborne. Results: The phytochemical analysis carried out on the flowers of R. arboreum Sm. ssp. nilagiricum (Zenker) Tagg showed the presence of phenols, saponins, steroids, tannin, xanthoprotein and coumarin. Conclusions:The present study suggested that the flower extracts of R. arboreum Sm. ssp. nilagiricum (Zenker) Tagg possess significant phytochemical constituents and it can be used as antimicrobial agents against clinically isolated pathogens.

  10. Preliminary phytochemical studies of the leaf extracts of Rhododendron arboreum Sm. ssp. nilagiricum (Zenker) Tagg

    Institute of Scientific and Technical Information of China (English)

    Solomon Kiruba; Mony Mahesh; Zachariah Miller Paul; Solomon Jeeva

    2012-01-01

    Objective: To examine the secondary metabolites present in the leaf extracts of Rhododendron arboreum Sm. ssp. nilagiricum (Zenker) Tagg. Methods: Phytochemical screening of the leaf extract was done to determine the phytochemical constituents in the various solvents studied.Results:nilagiricum (Zenker) Tagg. confirm the existence of secondary metabolites such as phenols, saponins and tannins. Conclusions: The study suggests that the leaf extracts of R. arboreum Sm. ssp. nilagiricum (Zenker) Tagg. can be best utilized in developing bioactive compounds against pathogenic infection. The phytochemical study carried out on the leaf extracts of R. arboreum Sm. ssp.

  11. Lactobacillus casei Zhang and Saccharomyces cerevisiae QH2-2 mixed culture technology research%Lactobacillus casei Zhang与Saccharomyces cerevisiae QH2-2混合培养工艺的研究

    Institute of Scientific and Technical Information of China (English)

    胡殿庚; 姚国强; 郭建林; 刘乔; 张雪梅; 韩琬; 赵旭; 张和平

    2016-01-01

    对Lactobacillus casei Zhang和Saccharomyces cerevisiae QH2-2的混合发酵工艺进行了研究.通过对氮源、发酵温度和接菌工艺的优化证实以大豆蛋白粉为氮源,30℃同时接菌的发酵工艺能获得更高的L.casei Zhang vv和S.cerevisiae QH2-2活菌数,对高密度发酵的发酵温度和接菌工艺进行优化证实,30℃同时接菌条件下,两株菌均获得最高活菌数.其中L.casei Zhang活菌数达到2.25× 1010mL-1,S.cerevisiae QH2-2活菌数达到6.21× 108 mL-1.冻干菌粉中L.casei Zhang的活菌数达到2.67× 1011g-1,S.cerevisiae QH2-2的活菌数达到1.23× 109 g-1.上述菌种的混合发酵为益生菌发酵剂和微生态制剂的制备提供了参考.

  12. Ability of Lactobacillus rhamnosus GAF01 to remove AFM1 in vitro and to counteract AFM1 immunotoxicity in vivo.

    Science.gov (United States)

    Abbès, Samir; Salah-Abbès, Jalila Ben; Sharafi, Hakimeh; Jebali, Rania; Noghabi, Kambiz Akbari; Oueslati, Ridha

    2013-01-01

    Aflatoxin M1 (AFM1) has been detected in many parts of the world both in raw milk and many dairy products, causing great economic losses and human disease. Unfortunately, there are few studies dealing with AFM1 immunotoxicity/interactions with lactic acid bacteria for potential application as a natural preventive agent. The aim of this study was to isolate (from dairy products) food-grade probiotic bacteria able to degrade/bind AFM1 in vitro and evaluate whether the same organism(s) could impart a protective role against AFM1-induced immunotoxicity in exposed Balb/c mice. Bacteria (Lactobacillus plantarum MON03 and L. rhamnosus GAF01) were isolated from Tunisian artisanal butter and then tested for abilities to eliminate AFM1 from phosphate-buffered saline (PBS) and reconstituted milk (containing 0.05, 0.10, and 0.20 µg AFM1/ml) after 0, 6, and 24 h at 37°C. Results showed that the selected bacteria could 'remove' AFM1 both in PBS and skimmed milk. The binding abilities of AFM1 by L. plantarum MON03 and L. rhamnosus GAF01 strains (at 10(8) CFU/ml) in PBS and reconstituted milk ranged, respectively, from 16.1-78.6% and 15.3-95.1%; overall, L. rhamnosus showed a better potential for removal than L. plantarum. 'Removal' appeared to be by simple binding; the bacteria/AFM1 complex was stable and only a very small proportion of mycotoxin was released back into the solution. L. rhamnosus GAF01 had the highest binding capacity and was selected for use in the in vivo study. Those results indicated that use of the organism prevented AFM1-induced effects on total white and red blood cells, and lymphocyte subtypes, after 15 days of host treatment. These studies clearly indicated that L. rhamnosus GAF01 was able to bind AFM1 in vitro and-by mechanisms that might also be related to a binding effect-counteract AFM1-induced immunotoxicity. Moreover, by itself, this bacterium was not toxic and could potentially be used as an additive in dairy products and in biotechnology for

  13. An Efficient DNA Extraction Method for Lactobacillus casei, a Difficult-to-Lyse Bacterium

    Directory of Open Access Journals (Sweden)

    Alimolaei

    2016-02-01

    Full Text Available Background There are several protocols to extract DNA from Lactobacillus spp. In the case of L. casei it is harder because of its especial and thick cell wall. Objectives In this study, nine DNA extraction protocols (by lysozyme treatment were evaluated and compared in two categories (traditional and kit-based protocols and an improved method was presented. Materials and Methods DNA quantity and quality was determined by spectrophotometry, agarose gel electrophoresis and polymerase chain reaction (PCR. Results The results revealed that the yield of extracted DNA differed by each protocol (5.8 - 17.1 μg/100 μL, but provided appropriate DNA for PCR amplification. The modified protocol offered the best total DNA extraction method when both quality (DNA purity; 1.54 μg and quantity (DNA yield; 17.1 μg were considered. Conclusions We suggest this protocol for effective and inexpensive DNA isolation from L. casei for downstream biological processes such as PCR.

  14. Lactobacillus casei strain Shirota protects against nonalcoholic steatohepatitis development in a rodent model.

    Science.gov (United States)

    Okubo, Hirofumi; Sakoda, Hideyuki; Kushiyama, Akifumi; Fujishiro, Midori; Nakatsu, Yusuke; Fukushima, Toshiaki; Matsunaga, Yasuka; Kamata, Hideaki; Asahara, Takashi; Yoshida, Yasuto; Chonan, Osamu; Iwashita, Misaki; Nishimura, Fusanori; Asano, Tomoichiro

    2013-12-01

    Gut microbiota alterations are associated with various disorders. In this study, gut microbiota changes were investigated in a methionine-choline-deficient (MCD) diet-induced nonalcoholic steatohepatitis (NASH) rodent model, and the effects of administering Lactobacillus casei strain Shirota (LcS) on the development of NASH were also investigated. Mice were divided into three groups, given the normal chow diet (NCD), MCD diet, or the MCD diet plus daily oral administration of LcS for 6 wk. Gut microbiota analyses for the three groups revealed that lactic acid bacteria such as Bifidobacterium and Lactobacillus in feces were markedly reduced by the MCD diet. Interestingly, oral administration of LcS to MCD diet-fed mice increased not only the L. casei subgroup but also other lactic acid bacteria. Subsequently, NASH development was evaluated based on hepatic histochemical findings, serum parameters, and various mRNA and/or protein expression levels. LcS intervention markedly suppressed MCD-diet-induced NASH development, with reduced serum lipopolysaccharide concentrations, suppression of inflammation and fibrosis in the liver, and reduced colon inflammation. Therefore, reduced populations of lactic acid bacteria in the colon may be involved in the pathogenesis of MCD diet-induced NASH, suggesting normalization of gut microbiota to be effective for treating NASH.

  15. Chemical characterization and immunomodulatory properties of polysaccharides isolated from probiotic Lactobacillus casei LOCK 0919

    Science.gov (United States)

    Górska, Sabina; Hermanova, Petra; Ciekot, Jarosław; Schwarzer, Martin; Srutkova, Dagmar; Brzozowska, Ewa; Kozakova, Hana; Gamian, Andrzej

    2016-01-01

    The Lactobacillus casei strain, LOCK 0919, is intended for the dietary management of food allergies and atopic dermatitis (LATOPIC® BIOMED). The use of a probiotic to modulate immune responses is an interesting strategy for solving imbalance problems of gut microflora that may lead to various disorders. However, the exact bacterial signaling mechanisms underlying such modulations are still far from being understood. Here, we investigated variations in the chemical compositions and immunomodulatory properties of the polysaccharides (PS), L919/A and L919/B, which are produced by L. casei LOCK 0919. By virtue of their chemical features, such PS can modulate the immune responses to third-party antigens. Our results revealed that L919/A and L919/B could both modulate the immune response to Lactobacillus planatarum WCFS1, but only L919/B could alter the response of THP-1 cells (in terms of tumor necrosis factor alpha production) to L. planatarum WCFS1 and Escherichia coli Nissle 1917. The comprehensive immunochemical characterization is crucial for the understanding of the biological function as well as of the bacteria–host and bacteria–bacteria cross-talk. PMID:27102285

  16. Bosom Buddies: The Symbiotic Relationship Between Infants and Bifidobacterium longum ssp. longum and ssp. infantis. Genetic and Probiotic Features.

    Science.gov (United States)

    Arboleya, Silvia; Stanton, Catherine; Ryan, C Anthony; Dempsey, Eugene; Ross, Paul R

    2016-01-01

    The intestinal microbiota is a complex community that plays an important role in human health from the initial steps of its establishment. Its microbial composition has been suggested to result from selective pressures imposed by the host and is modulated by competition among its members. Bifidobacterium longum is one of the most abundant species of the Bifidobacterium genus in the gut microbiota of healthy breast-fed infants and adults. The recent advancements of 'omics techniques have facilitated the genetic and functional studies of different gut microbiota members. They have revealed the complex genetic pathways used to metabolize different compounds that likely contribute to the competitiveness and persistence of B. longum in the colon. The discovery of a genomic island in B. longum ssp. infantis that encodes specific enzymes for the metabolism of human milk oligosaccharides suggests a specific ecological adaptation. Moreover, B. longum is widely used as probiotic, and beneficial effects in infant health have been reported in several studies. PMID:26934170

  17. Lactobacillus casei Shirota does not decrease the food allergic response to peanut extract in Brown Norway rats

    NARCIS (Netherlands)

    Jonge, J.D. de; Ezendam, J.; Knippels, L.M.J.; Penninks, A.H.; Pieters, R.; Loveren, H. van

    2008-01-01

    Probiotics are claimed to beneficially affect the immune system and their involvement in allergy prevention is being investigated extensively. However, the efficacy of probiotics in allergy prevention remains controversial. We investigated whether the probiotic Lactobacillus casei Shirota (LcS) coul

  18. Simultaneous chromate reduction and azo dye decolourization by Brevibacterium casei: Azo dye as electron donor for chromate reduction

    International Nuclear Information System (INIS)

    Chromate [Cr(VI)] and azo dyes are common pollutants which may co-exist in some industrial effluents. Hence studies of biological treatment of industrial wastewater should include investigation of the co-removal of these two pollutants. Brevibacterium casei, which can reduce Cr(VI) in the presence of the azo dye Acid Orange 7 (AO7) under nutrient-limiting condition, was isolated from a sewage sludge sample of a dyeing factory. Response surface methodology, which is commonly used to optimize growth conditions for food microorganisms to maximize product(s) yield, was used to determine the optimal conditions for chromate reduction and dye decolourization by B. casei. The optimal conditions were 0.24 g/L glucose, 3.0 g/L (NH4)2SO4 and 0.2 g/L peptone at pH 7 and 35 deg. C. The predicted maximum chromate reduction efficiencies and dye decolourization were 83.4 ± 0.6 and 40.7 ± 1.7%, respectively. A new mechanism was proposed for chromate reduction coupling with AO7 decolourization by B. casei. Under nutrient-limiting condition, AO7 was used as an e- donor by the reduction enzyme(s) of B. casei for the reduction of Cr(VI). The resulted Cr(III) then complexed with the oxidized AO7 to form a purple coloured intermediate.

  19. Simultaneous chromate reduction and azo dye decolourization by Brevibacterium casei: Azo dye as electron donor for chromate reduction

    Energy Technology Data Exchange (ETDEWEB)

    Ng, Tsz Wai; Cai Qinhong [Department of Biology, Chinese University of Hong Kong, Shatin, N.T. (Hong Kong); Wong, Chong-Kim [Department of Biology, Chinese University of Hong Kong, Shatin, N.T. (Hong Kong); Environmental Science Programme, Chinese University of Hong Kong, Shatin, N.T. (Hong Kong); Chow, Alex T. [Department of Biosystems Engineering, Clemson University, SC 29634 (United States); Department of Forestry and Natural Resources, Clemson University, SC 29634 (United States); Wong, Po-Keung, E-mail: pkwong@cuhk.edu.hk [Department of Biology, Chinese University of Hong Kong, Shatin, N.T. (Hong Kong); Environmental Science Programme, Chinese University of Hong Kong, Shatin, N.T. (Hong Kong)

    2010-10-15

    Chromate [Cr(VI)] and azo dyes are common pollutants which may co-exist in some industrial effluents. Hence studies of biological treatment of industrial wastewater should include investigation of the co-removal of these two pollutants. Brevibacterium casei, which can reduce Cr(VI) in the presence of the azo dye Acid Orange 7 (AO7) under nutrient-limiting condition, was isolated from a sewage sludge sample of a dyeing factory. Response surface methodology, which is commonly used to optimize growth conditions for food microorganisms to maximize product(s) yield, was used to determine the optimal conditions for chromate reduction and dye decolourization by B. casei. The optimal conditions were 0.24 g/L glucose, 3.0 g/L (NH{sub 4}){sub 2}SO{sub 4} and 0.2 g/L peptone at pH 7 and 35 deg. C. The predicted maximum chromate reduction efficiencies and dye decolourization were 83.4 {+-} 0.6 and 40.7 {+-} 1.7%, respectively. A new mechanism was proposed for chromate reduction coupling with AO7 decolourization by B. casei. Under nutrient-limiting condition, AO7 was used as an e{sup -} donor by the reduction enzyme(s) of B. casei for the reduction of Cr(VI). The resulted Cr(III) then complexed with the oxidized AO7 to form a purple coloured intermediate.

  20. Experimental and Pathalogical study of Pistacia atlantica, butyrate, Lactobacillus casei and their combination on rat ulcerative colitis model.

    Science.gov (United States)

    Gholami, Mahdi; Ghasemi-Niri, Seyedeh Farnaz; Maqbool, Faheem; Baeeri, Maryam; Memariani, Zahra; Pousti, Iraj; Abdollahi, Mohammad

    2016-06-01

    This study evaluated the effects of Pistacia atlantica (P. atlantica), butyrate, Lactobacillus casei (L. casei) and especially their combination therapy on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced rat colitis model. Rats were divided into seven groups. Four groups received oral P. atlantica, butyrate, L. casei and the combination of three agents for 10 consecutive days. The remaining groups were negative and positive controls and a sham group. Macroscopic and histopathological examinations were carried out along with determination of the specific biomarker of colonic oxidative stress, the myeloperoxidase (MPO). Compared with controls, the combination therapy exhibited a significant alleviation of colitis in terms of pathological scores and reduction of MPO activity (55%, p=0.0009). Meanwhile, the macroscopic appearance such as stool consistency, tissue and histopathological scores (edema, necrosis and neutrophil infiltration) were improved. Although single therapy by each P. atlantica, butyrate, and L. casei was partially beneficial in reduction of colon oxidative stress markers, the combination therapy was much more effective. In conclusion, the combination therapy was able to reduce the severity of colitis that is clear from biochemical markers. Future studies have to focus on clinical effects of this combination in management of human ulcerative colitis. Further molecular and signaling pathway studies will help to understand the mechanisms involved in the treatment of colitis and inflammatory diseases. PMID:26972417

  1. Studies on Mucosal Immunity Induced by Transmissible Gastroenteritis Virus Nucleocapsid Protein Recombinant Lactobacillus casei in Mice and Sow

    Institute of Scientific and Technical Information of China (English)

    WANG Gui-hua; HOU Xi-lin; YU Li-yun; LIU Jian-kui; WEI Chun-hua

    2009-01-01

    Mucosal immunity plays an important role in protecting pigs against transmissible gastroenteritis virus (TGEV) infection. To elicit mucosal immune response against TGEV, we developed a surface antigen display system using the poly-γ-glutamate synthetase A (pgsA) protein of Bacillus subtilis as an anchoring matrix to express recombinant fusion proteins of pgsA and nucleocapsid protein of TGEV in Lactobacillus casei. Surface location of fusion protein was verified by ELISA and indirect immunofluorescence test. Oral and intranasal inoculations of pregnant sow and mice with recombinant L. casei resulted in high levels of serum immunoglobulin G (IgG) and secretory immunogiobulin A (sIgA) against recombinant N protein as demonstrated by ELISA. More importantly, the level of specific slgA in colostrum significantly increased compared with that of IgG. The serum lgG levels of the piglets increased after suckling coiostrum produced by sows was previously inoculated with recombinant L. casei. These results indicate that immunization with recombinant L. casei expressing TGEV N protein on its surface elicited high levels of specific slgA and circulating lgG against TGEV N protein.

  2. Lactobacillus casei prevents the development of dextran sulphate sodium-induced colitis in Toll-like receptor 4 mutant mice.

    Science.gov (United States)

    Chung, Y W; Choi, J H; Oh, T-Y; Eun, C S; Han, D S

    2008-01-01

    Probiotics, defined as live or attenuated bacteria or bacterial products, confer a significant health benefit to the host. Recently, they have been shown to be useful in the treatment of chronic inflammatory bowel disease and infectious colitis. In this study, we investigated the effect of probiotics on the development of experimental colitis using Toll-like receptor 4 (TLR-4) mutant (lps-/lps-) mice. TLR-4(lps-/lps-) and wild-type (WT) mice were given 2.5% dextran sulphate sodium (DSS) in drinking water to induce colitis with or without Lactobacillus casei pretreatment. Clinical and histological activity of DSS-colitis was attenuated markedly both in TLR-4(lps-/lps-) and WT mice pretreated with L. casei. Interestingly, histological activity was less severe in TLR-4(lps-/lps-) mice than in WT mice. The levels of myeloperoxidase activity and interleukin (IL)-12p40 were attenuated in pretreated TLR-4(lps-/lps-) mice after DSS administration. By contrast, transforming growth factor (TGF)-beta and IL-10 mRNA and protein expressions were increased markedly in pretreated TLR-4(lps-/lps-) mice. The current results suggest that L. casei has a preventive effect in the development of acute DSS-induced colitis and its action depends largely upon TLR-4 status. L. casei modulates the expression of inflammatory cytokines and down-regulates neutrophilic infiltration in the case of incomplete TLR-4 complex signalling. PMID:18005362

  3. Four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® shows modest effect on triacylglycerol in young healthy adults

    DEFF Research Database (Denmark)

    Bjerg, Anne Toksvig; Kristensen, Mette Bredal; Ritz, Christian;

    2015-01-01

    The microbiota has been shown to have the potential to affect appetite and blood lipids positively in animal studies. We investigated if four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® (L. casei W8) had an effect on subjective appetite sensation, ad libitum...... were measured. Additionally fasting blood lipids and fatty acids concentrations were measured. Sixteen piglets were randomised into two groups: L. casei W8 (1010 cfu/day) as top dressing on morning fed or no treatment. After two weeks piglets were sacrificed and tissue from ileum, jejunum and skeletal...

  4. An abstract class loader for the SSP and its implementation in TL.

    Energy Technology Data Exchange (ETDEWEB)

    Wickstrom, Gregory Lloyd; Winter, Victor Lono (University of Nebraska at Omaha, Omaha, NE); Fraij, Fares (University of Texas at El Paso, El Paso, TX); Roach, Steve (University of Texas at El Paso, El Paso, TX); Beranek, Jason (University of Nebraska at Omaha, Omaha, NE)

    2004-08-01

    The SSP is a hardware implementation of a subset of the JVM for use in high consequence embedded applications. In this context, a majority of the activities belonging to class loading, as it is defined in the specification of the JVM, can be performed statically. Static class loading has the net result of dramatically simplifying the design of the SSP as well as increasing its performance. Due to the high consequence nature of its applications, strong evidence must be provided that all aspects of the SSP have been implemented correctly. This includes the class loader. This article explores the possibility of formally verifying a class loader for the SSP implemented in the strategic programming language TL. Specifically, an implementation of the core activities of an abstract class loader is presented and its verification in ACL2 is considered.

  5. Characterization and site-directed mutagenesis of Wzb, an O-phosphatase from Lactobacillus rhamnosus

    Directory of Open Access Journals (Sweden)

    Gilbert Christophe

    2008-04-01

    Full Text Available Abstract Background Reversible phosphorylation events within a polymerisation complex have been proposed to modulate capsular polysaccharide synthesis in Streptococcus pneumoniae. Similar phosphatase and kinase genes are present in the exopolysaccharide (EPS biosynthesis loci of numerous lactic acid bacteria genomes. Results The protein sequence deduced from the wzb gene in Lactobacillus rhamnosus ATCC 9595 reveals four motifs of the polymerase and histidinol phosphatase (PHP superfamily of prokaryotic O-phosphatases. Native and modified His-tag fusion Wzb proteins were purified from Escherichia coli cultures. Extracts showed phosphatase activity towards tyrosine-containing peptides. The purified fusion protein Wzb was active on p-nitrophenyl-phosphate (pNPP, with an optimal activity in presence of bovine serum albumin (BSA 1% at pH 7.3 and a temperature of 75°C. At 50°C, residual activity decreased to 10 %. Copper ions were essential for phosphatase activity, which was significantly increased by addition of cobalt. Mutated fusion Wzb proteins exhibited reduced phosphatase activity on p-nitrophenyl-phosphate. However, one variant (C6S showed close to 20% increase in phosphatase activity. Conclusion These characteristics reveal significant differences with the manganese-dependent CpsB protein tyrosine phosphatase described for Streptococcus pneumoniae as well as with the polysaccharide-related phosphatases of Gram negative bacteria.

  6. Psychoactive bacteria Lactobacillus rhamnosus (JB-1) elicits rapid frequency facilitation in vagal afferents.

    Science.gov (United States)

    Perez-Burgos, Azucena; Wang, Bingxian; Mao, Yu-Kang; Mistry, Bhavik; McVey Neufeld, Karen-Anne; Bienenstock, John; Kunze, Wolfgang

    2013-01-15

    Mounting evidence supports the influence of the gut microbiome on the local enteric nervous system and its effects on brain chemistry and relevant behavior. Vagal afferents are involved in some of these effects. We previously showed that ingestion of the probiotic bacterium Lactobacillus rhamnosus (JB-1) caused extensive neurochemical changes in the brain and behavior that were abrogated by prior vagotomy. Because information can be transmitted to the brain via primary afferents encoded as neuronal spike trains, our goal was to record those induced by JB-1 in vagal afferents in the mesenteric nerve bundle and thus determine the nature of the signals sent to the brain. Male Swiss Webster mice jejunal segments were cannulated ex vivo, and serosal and luminal compartments were perfused separately. Bacteria were added intraluminally. We found no evidence for translocation of labeled bacteria across the epithelium during the experiment. We recorded extracellular multi- and single-unit neuronal activity with glass suction pipettes. Within minutes of application, JB-1 increased the constitutive single- and multiunit firing rate of the mesenteric nerve bundle, but Lactobacillus salivarius (a negative control) or media alone were ineffective. JB-1 significantly augmented multiunit discharge responses to an intraluminal distension pressure of 31 hPa. Prior subdiaphragmatic vagotomy abolished all of the JB-1-evoked effects. This detailed exploration of the neuronal spike firing that encodes behavioral signaling to the brain may be useful to identify effective psychoactive bacteria and thereby offer an alternative new perspective in the field of psychiatry and comorbid conditions.

  7. Safety Study of an Antimicrobial Peptide Lactocin 160, Produced by the Vaginal Lactobacillus rhamnosus

    Directory of Open Access Journals (Sweden)

    Sara E. Dover

    2007-01-01

    Full Text Available Objective. To evaluate the safety of the antimicrobial peptide, lactocin 160. Methods. Lactocin 160, a product of vaginal probiotic Lactobacillus rhamnosus 160 was evaluated for toxicity and irritation. An in vitro human organotypic vaginal-ectocervical tissue model (EpiVaginal was employed for the safety testing by determining the exposure time to reduce tissue viability to 50% (ET-50. Hemolytic activity of lactocin160 was tested using 8% of human erythrocyte suspension. Susceptibility of lactobacilli to lactocin160 was also studied. Rabbit vaginal irritation (RVI model was used for an in vivo safety evaluation. Results. The ET-50 value was 17.5 hours for lactocin 160 (4.9 hours for nonoxynol 9, N9. Hemolytic activity of lactocin 160 was 8.2% (N9 caused total hemolysis. Lactobacilli resisted to high concentrations of peptide preparation. The RVI model revealed slight vaginal irritation. An average irritation index grade was evaluated as “none.” Conclusions. Lactocin 160 showed minimal irritation and has a good potential for intravaginal application.

  8. Lactobacillus rhamnosus GG-supplemented formula expands butyrate-producing bacterial strains in food allergic infants

    Science.gov (United States)

    Berni Canani, Roberto; Sangwan, Naseer; Stefka, Andrew T; Nocerino, Rita; Paparo, Lorella; Aitoro, Rosita; Calignano, Antonio; Khan, Aly A; Gilbert, Jack A; Nagler, Cathryn R

    2016-01-01

    Dietary intervention with extensively hydrolyzed casein formula supplemented with Lactobacillus rhamnosus GG (EHCF+LGG) accelerates tolerance acquisition in infants with cow's milk allergy (CMA). We examined whether this effect is attributable, at least in part, to an influence on the gut microbiota. Fecal samples from healthy controls (n=20) and from CMA infants (n=19) before and after treatment with EHCF with (n=12) and without (n=7) supplementation with LGG were compared by 16S rRNA-based operational taxonomic unit clustering and oligotyping. Differential feature selection and generalized linear model fitting revealed that the CMA infants have a diverse gut microbial community structure dominated by Lachnospiraceae (20.5±9.7%) and Ruminococcaceae (16.2±9.1%). Blautia, Roseburia and Coprococcus were significantly enriched following treatment with EHCF and LGG, but only one genus, Oscillospira, was significantly different between infants that became tolerant and those that remained allergic. However, most tolerant infants showed a significant increase in fecal butyrate levels, and those taxa that were significantly enriched in these samples, Blautia and Roseburia, exhibited specific strain-level demarcations between tolerant and allergic infants. Our data suggest that EHCF+LGG promotes tolerance in infants with CMA, in part, by influencing the strain-level bacterial community structure of the infant gut. PMID:26394008

  9. Microencapsulated Lactobacillus rhamnosus GG powders: relationship of powder physical properties to probiotic survival during storage.

    Science.gov (United States)

    Ying, Dan Yang; Phoon, Mei Chi; Sanguansri, Luz; Weerakkody, Rangika; Burgar, Iko; Augustin, Mary Ann

    2010-01-01

    Freeze-dried commercial Lactobacillus rhamnosus GG (LGG) were encapsulated in an emulsion-based formulation stabilized by whey protein and resistant starch and either spray-dried or freeze-dried to produce probiotic microcapsules. There was no difference in loss of probiotics viability after spray drying or freeze drying. Particle size, morphology, moisture sorption, and water mobility of the powder microcapsules were examined. Particle size analysis and scanning electron microscopy showed that spray-dried LGG microcapsules (SDMC) were small spherical particles, whereas freeze-dried LGG microcapsules (FDMC) were larger nonspherical particles. Moisture sorption isotherms obtained using dynamic vapor sorption showed a slightly higher water uptake in spray-dried microcapsules. The effect of water mobility, as measured by nuclear magnetic resonance (NMR) spectroscopy, at various water activities (a(w) 0.32, 0.57, and 0.70) and probiotic viability during storage at 25 °C was also examined. Increasing the relative humidity of the environment at which the samples were stored caused an increase in water mobility and the rate of loss in viability. The viability data during storage indicated that SDMC had better storage stability compared to FDMC. Although more water was adsorbed for spray-dried than freeze-dried microcapsules, water mobility was similar for corresponding storage conditions because there was a stronger water-binding energy for spray-dried microcapsule. This possibly accounted for the improved survival of probiotics in spray-dried microcapsules. PMID:21535593

  10. Microencapsulated Lactobacillus rhamnosus GG powders: relationship of powder physical properties to probiotic survival during storage.

    Science.gov (United States)

    Ying, Dan Yang; Phoon, Mei Chi; Sanguansri, Luz; Weerakkody, Rangika; Burgar, Iko; Augustin, Mary Ann

    2010-01-01

    Freeze-dried commercial Lactobacillus rhamnosus GG (LGG) were encapsulated in an emulsion-based formulation stabilized by whey protein and resistant starch and either spray-dried or freeze-dried to produce probiotic microcapsules. There was no difference in loss of probiotics viability after spray drying or freeze drying. Particle size, morphology, moisture sorption, and water mobility of the powder microcapsules were examined. Particle size analysis and scanning electron microscopy showed that spray-dried LGG microcapsules (SDMC) were small spherical particles, whereas freeze-dried LGG microcapsules (FDMC) were larger nonspherical particles. Moisture sorption isotherms obtained using dynamic vapor sorption showed a slightly higher water uptake in spray-dried microcapsules. The effect of water mobility, as measured by nuclear magnetic resonance (NMR) spectroscopy, at various water activities (a(w) 0.32, 0.57, and 0.70) and probiotic viability during storage at 25 °C was also examined. Increasing the relative humidity of the environment at which the samples were stored caused an increase in water mobility and the rate of loss in viability. The viability data during storage indicated that SDMC had better storage stability compared to FDMC. Although more water was adsorbed for spray-dried than freeze-dried microcapsules, water mobility was similar for corresponding storage conditions because there was a stronger water-binding energy for spray-dried microcapsule. This possibly accounted for the improved survival of probiotics in spray-dried microcapsules.

  11. Effect of probiotic Lactobacillus rhamnosus GG intervention on global serum lipidomic profiles in healthy adults

    Institute of Scientific and Technical Information of China (English)

    Riina A Kekkonen; Marko Sysi-Aho; Tuulikki Sepp(a)nen-Laakso; Ilkka Julkunen; Heikki Vapaatalo; Matej Ore(s)i(c); Riitta Korpela

    2008-01-01

    AIM:To investigate the effect of three weeks' intervention with a probiotic Lactobacillus rhamnosus GG (LGG) bacteria on global serum lipidomic profiles and evaluate whether the changes in inflammatory variables (CRP,TNF-α and IL-6) are reflected in the global lipidomic profiles of healthy adults.METHODS:We performed UPLC/MS-based global lipidomic platform analysis of serum samples (n = 26)in a substudy of a randomised,double-blind,placebocontrolled 3-wk clinical intervention trial investigating the immunomodulatory effects of probiotics in healthy adults.RESULTS:A total of 407 lipids were identified,corresponding to 13 different lipid classes.Serum samples showed decreases in the levels of lysophosphatidylcholines (LysoGPCho),sphingomyelins (SM) and several glycerophosphatidylcholines (GPCho),while triacylglycerols (TAG) were mainly increased in the probiotic LGG group during the intervention.Among the inflammatory variables,IL-6 was moderately associated by changes in global lipidomic profiles,with the top-ranked lipid associated with IL-6 being the proinflammatory LysoGPCho (20:4).There was a weak association between the lipidomic profiles and the two other inflammatory markers,TNF-α and CRP.CONCLUSION:This was the first study to investigate the effects of probiotic intervention on global lipidomic profiles in humans.There are indications that probiotic LGG intervention may lead to changes in serum global lipid profiles,as reflected in decreased GPCho,LysoGPCho and SM as well as mainly increased TAG.

  12. Evaluation of the response of Lactobacillus rhamnosus VTT E-97800 to sucrose-induced osmotic stress.

    Science.gov (United States)

    Sunny-Roberts, E O; Knorr, D

    2008-02-01

    Environmental osmotic changes are one of the stresses live probiotics may encounter either in their natural habitats or as a result of usage in food formulations and processing. Response to osmotic stress, induced by sucrose, of the probiotic strain Lactobacillus rhamnosus VTT E-97800 (E800) was investigated. The fluorescence-based approach used, by combined staining with caboxyfluorescein (cFDA) and propidium iodide (PI) could give insights on the osmotic-induced changes of microbial esterase activity and membrane integrity; also the extrusion of intracellular accumulated carboxyfluorescein (cF) upon energizing with glucose. Comparison of the flowcytometric viability assessment with the conventional culture techniques revealed that sucrose-stressed cells had a slight loss of culturability (logN/N(0) approximately -0.3) at 1.2 and 1.5M sucrose concentration though they could perform an enzymatic conversion of cFDA into cF. The presence of such metabolically active bacteria in food might be critical as they may excrete toxic or food spoilage metabolites. Moreover, the perturbation of cF extrusion activities became a limiting factor for reproductive capacities. There was no change in the cell morphology. These results proved the ability of the strain of study to tolerate sucrose, even at extreme concentrations and these must be taken into consideration for its usage in the formulation/processing of sugar-based foods, e.g. jams, candies, etc.

  13. Effects of Lactobacillus rhamnosus GG supplementation on cow's milk allergy in a mouse model

    Directory of Open Access Journals (Sweden)

    Thang Cin L

    2011-12-01

    Full Text Available Abstract Background Cow's milk allergy (CMA is one of the most prevalent human food-borne allergies, particularly in infants and young children from developed countries. Our study aims to evaluate the effects of Lactobacillus rhamnosus GG (LGG administration on CMA development using whole cow's milk proteins (CMP sensitized Balb/C mice by two different sensitization methods. Methods LGG supplemented mice were either sensitized orally with CMP and cholera toxin B-subunit (CTB as adjuvant, or intraperitoneally (IP with CMP but without the adjuvant. Mice were then orally challenged with CMP and allergic responses were accessed by monitoring hypersensitivity scores, measuring the levels of CMP-specific immunoglobulins (IgG1, IgG2a and IgG and total IgE from sera, and cytokines (IL-4 and IFN-γ from spleen lysates. Results Sensitization to CMP was successful only in IP sensitized mice, but not in orally sensitized mice with CMP and CTB. Interestingly, LGG supplementation appeared to have reduced cow's milk allergy (CMA in the IP group of mice, as indicated by lowered allergic responses. Conclusions Adjuvant-free IP sensitization with CMP was successful in inducing CMA in the Balb/C mice model. LGG supplementation favourably modulated immune reactions by shifting Th2-dominated trends toward Th1-dominated responses in CMP sensitized mice. Our results also suggest that oral sensitization by the co-administration of CMP and CTB, as adjuvant, might not be appropriate to induce CMA in mice.

  14. Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice

    Science.gov (United States)

    Zhang, Zhen; Zhou, Zhigang; Li, Yu; Zhou, Linkang; Ding, Qianwen; Xu, Li

    2016-01-01

    The fibroblast cell line of 3T3-L1 was used as a cell model for screening and evaluating the feasibility of probiotic components in improving animal lipid metabolisms. The extracts from 12 Lactobacillus strains caused significantly reduced triacylglycerol (TAG) accumulation but with severe inflammation induction in 3T3-L1 adipocytes. Interestingly, exopolysaccharides (EPS) from LGG (Lactobacillus rhamnosus GG) significantly decreased the TAG accumulation without any inflammation. The anti-obesity effect of EPS was confirmed in high-fat-diets feeding mice. Fat pads of mice injected with EPS (50 mg/kg) every two days for two weeks were significantly reduced with much smaller adipocytes, compared with the counterparts. The levels of TAG and cholesterol ester in liver, as well as serum TAG, were decreased in EPS injected mice. In addition, down-regulated inflammation was observed in adipose tissue and liver. Interestingly, the expression of TLR2 in adipose tissue and 3T3-L1 cells was significantly increased by EPS addition. Moreover, the reverse of TAG accumulation in TLR2 knockdown 3T3-L1 in the presence of EPS confirmed that the inhibition effect of EPS on adipogenesis was mediated by TLR2. EPS from LGG has the potential for therapeutic development to intervene lipid metabolic disorders in mammals. PMID:27786292

  15. Lactobacillus rhamnosus GG-supplemented formula expands butyrate-producing bacterial strains in food allergic infants.

    Science.gov (United States)

    Berni Canani, Roberto; Sangwan, Naseer; Stefka, Andrew T; Nocerino, Rita; Paparo, Lorella; Aitoro, Rosita; Calignano, Antonio; Khan, Aly A; Gilbert, Jack A; Nagler, Cathryn R

    2016-03-01

    Dietary intervention with extensively hydrolyzed casein formula supplemented with Lactobacillus rhamnosus GG (EHCF+LGG) accelerates tolerance acquisition in infants with cow's milk allergy (CMA). We examined whether this effect is attributable, at least in part, to an influence on the gut microbiota. Fecal samples from healthy controls (n=20) and from CMA infants (n=19) before and after treatment with EHCF with (n=12) and without (n=7) supplementation with LGG were compared by 16S rRNA-based operational taxonomic unit clustering and oligotyping. Differential feature selection and generalized linear model fitting revealed that the CMA infants have a diverse gut microbial community structure dominated by Lachnospiraceae (20.5±9.7%) and Ruminococcaceae (16.2±9.1%). Blautia, Roseburia and Coprococcus were significantly enriched following treatment with EHCF and LGG, but only one genus, Oscillospira, was significantly different between infants that became tolerant and those that remained allergic. However, most tolerant infants showed a significant increase in fecal butyrate levels, and those taxa that were significantly enriched in these samples, Blautia and Roseburia, exhibited specific strain-level demarcations between tolerant and allergic infants. Our data suggest that EHCF+LGG promotes tolerance in infants with CMA, in part, by influencing the strain-level bacterial community structure of the infant gut. PMID:26394008

  16. Development of healthy whey drink with Lactobacillus rhamnosus, Bifidobacterium bifidum and Propionibacterium freudenreichii subsp. shermanii

    Directory of Open Access Journals (Sweden)

    T.K. Maity

    2008-12-01

    Full Text Available Whey beverage was prepared by utilizing Lactobacillus rhamnosus NCDO 243, Bifidobacterium bifidum NCDO 2715 and Propionibacterium freudenreichii subsp. shermanii MTCC 1371 in order to make a fermented probiotic healthy drink. The product made with 4 % mixed culture (1:1:1 inoculated (initial count - lactobacilli 6.2 x 107 CFU/mL, bifidobacteria 5.4 x 107 CFU/mL, propionibacteria 3.9 x 107 CFU/mL in deprotienized whey (4.6 % lactose, 0.62 % ash, 0.48 % fat and 0.5 % protein adjusted to pH 6.4 and incubated at 37 °C for 8 h has a good technological and dietetic criteria required for a probiotic product. Total bacterial count, lactobacilli count, bifidobacteria count, propionibacteria count, titratable acidity, β-D galactosidase activity, concentration of lactic acid and sensory properties were monitored during storage period. The whey beverage fermented for 8 h and prepared with 4 % inoculum of mixed culture (1:1:1 met the probiotic criterion by maintaining each type of bacterial population at counts greater than 108 CFU/mL up to 10 days of storage period. The titratable acidity as well as sensory properties did not change appreciably during first 7 days of storage. At the end of 15 days of storage, slight acidification was detected, although the beverage still retained an acceptable flavour.

  17. Cariogenic effects of probiotic Lactobacillus rhamnosus GG in a dental biofilm model.

    Science.gov (United States)

    Schwendicke, F; Dörfer, C; Kneist, S; Meyer-Lueckel, H; Paris, S

    2014-01-01

    Probiotic bacteria have been suggested to inhibit Streptococcus mutans (SM) and thus prevent dental caries. However, supporting evidence is weak and probiotic species might be cariogenic themselves. Thus, we compared and combined the probiotic Lactobacillus rhamnosus GG (LGG) with SM and analysed the resulting mineral loss (ΔZ) in dental tissues. We simulated three biofilm compositions (SM, LGG, SM × LGG), two lesion sites (smooth enamel, dentin cavity) and two nutrition supply frequencies (twice/day, 6 times/day) in a multi-station, continuous-culture biofilm model. A total of 240 bovine enamel and dentin samples were cut, polished and embedded. All experimental procedures were performed in independent duplicates, with 10 samples being allocated to each group for each experiment (final sample size n = 20/group). Biofilms were cultured on the specimens and supplied with 2% sucrose medium and artificial saliva in consecutive pulses. After 10 days, ΔZ and bacterial numbers were assessed. SM × LGG biofilms caused significantly increased ΔZ compared with SM or LGG biofilms (p biofilms of different species (p > 0.05, ANOVA). Frequent nutrition supply significantly increased bacterial numbers (p Biofilms in dentin cavities compared to smooth enamel harboured significantly more bacteria (p < 0.05). LGG induced mineral loss especially in dentin cavities and under highly cariogenic conditions. LGG did not have inhibitory effects on SM, but rather contributed to the caries process in vitro.

  18. Effective prophylaxis against rotavirus diarrhea using a combination of Lactobacillus rhamnosus GG and antibodies

    Directory of Open Access Journals (Sweden)

    Hammarström Lennart

    2007-09-01

    Full Text Available Abstract Background Rotavirus is a worldwide cause of infectious infantile diarrhea that claims over 600,000 lives annually. Recently, two new vaccine candidates have been developed but their efficacy in developing countries, still remains to be proven. Oral delivery of specific immunoglobulins provides passive immunity and is a fast acting treatment for rotavirus diarrhea. Probiotic bacteria have also gained considerable attention lately as treatment for rotavirus diarrhea. Here we report an evaluation of the therapeutic potential of different probiotics and their combination with anti – rotavirus antibodies in a mouse model of rotavirus diarrhea. Results Of the six probiotic bacteria tested, Lactobacillus rhamnosus strain GG had the strongest influence in reducing prevalence, duration and severity of diarrhea and was therefore chosen for combination treatment with immunoglobulins. The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines. Conclusion The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient. These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

  19. A Novel Bluetooth Man-In-The-Middle Attack Based On SSP using OOB Association model

    OpenAIRE

    Saravanan, K.; Vijayanand, L.; Negesh, R. K.

    2012-01-01

    As an interconnection technology, Bluetooth has to address all traditional security problems, well known from the distributed networks. Moreover, as Bluetooth networks are formed by the radio links, there are also additional security aspects whose impact is yet not well understood. In this paper, we propose a novel Man-In-The-Middle (MITM) attack against Bluetooth enabled mobile phone that support Simple Secure Pairing(SSP). From the literature it was proved that the SSP association models su...

  20. Evaluación de la calidad funcional y sensorial en cultivares de Triticum aestivum ssp. vulgare y ssp. spelta en cultivo ecológico

    OpenAIRE

    Vargas Kostiuk, María Eugenia

    2016-01-01

    En esta Tesis Doctoral se ha estudiado la influencia del cultivar sobre el comportamiento reológico y panadero de cinco cultivares de trigo sembrados en el mismo año y en el mismo ambiente, en condiciones de cultivo ecológico. Tres de ellos eran de trigo panadero (Triticum aestivum ssp. vulgare), ‘Bonpain’, ‘Craklin’ y ‘Sensas’ y los otros dos de trigo espelta (Triticum aestivum ssp. spelta), ‘Espelta Álava’ y ‘Espelta Navarra’. Actualmente, el alohexaploide trigo panadero (2n=6x=42 genomio A...

  1. The Antifungal Properties of Lactobacillus casei AST18 and Its Application as Adjunct Culture in Yogurt%Lactobacillus casei AST18抗真菌特性及其在酸奶保鲜中的应用

    Institute of Scientific and Technical Information of China (English)

    李红娟; 刘鹭; 张书文; 孔凡丕; 孙卓; 吕加平

    2011-01-01

    [目的]考察来源于中国传统发酵制品中乳酸菌的抑真菌特性,研究其在酸奶贮藏中的抑制霉菌效果.[方法]高效液相色谱法( HPLC)检测7株具有抗真菌效果的乳杆菌发酵液中苯乳酸(PLA)含量,研究PLA与乳杆菌发酵液抗真菌活性的相关性.选取抑菌活性最强的菌株Lactobacillus casei AST18进行抑菌特性研究.Lactobacillus casei AST18分别以2%、4%、6%、8%接种量作为辅助发酵剂添加至酸奶发酵工艺中,成品酸奶贮藏期间,监测酸奶中霉菌生长状况,检测酸奶理化指标并进行感官评定.[结果]乳杆菌发酵液中苯乳酸含量与其抑真菌直径间相关关系不显著.Lactobacillus casei AST18发酵上清液经胃蛋白酶和胰蛋白酶处理后不影响其抑菌活性,而环境pH对其抑菌活性的影响显著,热处理可使其丧失抑菌活性.Lactobacillus casei AST18以2%添加量作为辅助发酵剂应用在酸奶中可抑制霉菌菌丝生长和孢子生成,且对酸奶产品风味、感官品质无显著影响.[结论]Lactobacillus casei AST18具有较好的抑制霉菌生长能力,应用在酸奶中具有显著的防霉保鲜效果.%[Objective] The antifungal activities of lactic acid bacteria (LAB) isolated from Chinese traditional fermented products were detected. The selected strain was used as adjunct culture in yogurt to control the fungal pollution. [Method] The production of phenyl lactic acid (PLA) was detected with HPLC and the correlation between PL A and the antifungal activities was studied. Then the antifungal properties of Lactobacillus casei AST 18 culture supernatants were detected. Lactobacillus casei AST 18 was used as adjunct culture in the yogurt production process. Four different inoculums of AST 18, 2%, 4%, 6%, 8% and a control group which used the commercial starter only were added in yogurt. After fermentation, sensory evaluation, physical and chemical indicators, the fungal growth conditions were

  2. Immobilization of Lactobacillus rhamnosus in mesoporous silica-based material: An efficiency continuous cell-recycle fermentation system for lactic acid production.

    Science.gov (United States)

    Zhao, Zijian; Xie, Xiaona; Wang, Zhi; Tao, Yanchun; Niu, Xuedun; Huang, Xuri; Liu, Li; Li, Zhengqiang

    2016-06-01

    Lactic acid bacteria immobilization methods have been widely used for lactic acid production. Until now, the most common immobilization matrix used is calcium alginate. However, Ca-alginate gel disintegrated during lactic acid fermentation. To overcome this deficiency, we developed an immobilization method in which Lactobacillus rhamnosus cells were successfully encapsulated into an ordered mesoporous silica-based material under mild conditions with a high immobilization efficiency of 78.77% by using elemental analysis. We also optimized the cultivation conditions of the immobilized L. rhamnosus and obtained a high glucose conversion yield of 92.4%. Furthermore, L. rhamnosus encapsulated in mesoporous silica-based material exhibited operational stability during repeated fermentation processes and no decrease in lactic acid production up to 8 repeated batches.

  3. Immobilization of Lactobacillus rhamnosus in mesoporous silica-based material: An efficiency continuous cell-recycle fermentation system for lactic acid production.

    Science.gov (United States)

    Zhao, Zijian; Xie, Xiaona; Wang, Zhi; Tao, Yanchun; Niu, Xuedun; Huang, Xuri; Liu, Li; Li, Zhengqiang

    2016-06-01

    Lactic acid bacteria immobilization methods have been widely used for lactic acid production. Until now, the most common immobilization matrix used is calcium alginate. However, Ca-alginate gel disintegrated during lactic acid fermentation. To overcome this deficiency, we developed an immobilization method in which Lactobacillus rhamnosus cells were successfully encapsulated into an ordered mesoporous silica-based material under mild conditions with a high immobilization efficiency of 78.77% by using elemental analysis. We also optimized the cultivation conditions of the immobilized L. rhamnosus and obtained a high glucose conversion yield of 92.4%. Furthermore, L. rhamnosus encapsulated in mesoporous silica-based material exhibited operational stability during repeated fermentation processes and no decrease in lactic acid production up to 8 repeated batches. PMID:26803707

  4. Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Wal Jean-Michel

    2007-04-01

    Full Text Available Abstract Background Lactic acid bacteria (LAB are attractive tools to deliver therapeutic molecules at the mucosal level. The model LAB Lactococcus lactis has been intensively used to produce and deliver such heterologous proteins. However, compared to recombinant lactococci, lactobacilli offer some advantages such as better survival in the digestive tract and immunomodulatory properties. Here, we compared different strategies to optimize the production of bovine β-lactoglobulin (BLG, a major cow's milk allergen, in the probiotic strain Lactobacillus casei BL23. Results Using a nisin-inducible plasmid system, we first showed that L. casei BL23 strain could efficiently secrete a reporter protein, the staphylococcal nuclease (Nuc, with the lactococcal signal peptide SPUsp45 fused to its N-terminus. The fusion of SPUsp45 failed to drive BLG secretion but led to a 10-fold increase of intracellular BLG production. Secretion was significantly improved when the synthetic propeptide LEISSTCDA (hereafter called LEISS was added to the N-terminus of the mature moiety of BLG. Secretion rate of LEISS-BLG was 6-fold higher than that of BLG alone while intracellular production reached then about 1 mg/L of culture. The highest yield of secretion was obtained by using Nuc as carrier protein. Insertion of Nuc between LEISS and BLG resulted in a 20-fold increase in BLG secretion, up to 27 μg/L of culture. Furthermore, the lactococcal nisRK regulatory genes were integrated into the BL23 chromosome. The nisRK insertion allowed a decrease of BLG synthesis in uninduced cultures while BLG production increased by 50% after nisin induction. Moreover, modification of the induction protocol led to increase the proportion of soluble BLG to around 74% of the total BLG production. Conclusion BLG production and secretion in L. casei were significantly improved by fusions to a propeptide enhancer and a carrier protein. The resulting recombinant strains will be further tested

  5. Effects of formulation variables on viability of L. casei loaded in whey protein-Ca alginate microparticles in simulated in vivo conditions

    OpenAIRE

    Smilkov, Katarina; Petrusevska Tozi, Lidija; Petreska Ivanovska, Tanja; Geskovski, Nikola; Petkovska, Rumenka; Glavas Dodov, Marija; Baceva, Katerina; Dimitrovski, Dejan; MLADENOVSKA, KRISTINA

    2011-01-01

    The objective of this work was to assess the influence of formulation variables of L. casei loaded whey protein-Ca-alginate microparticles on probiotic survival under different conditions, representing simulated in vivo environment.

  6. Instruments for oral disease-intervention strategies : recombinant Lactobacillus casei expressing tetanus toxin fragment C for vaccination or myelin proteins for oral tolerance induction in multiple sclerosis

    NARCIS (Netherlands)

    Maassen, C.B.M.; Laman, J.D.; Heijne den Bak-Glashouwer, M.J.; Tielen, F.J.; Holten-Neelen, J.C.P.A. van; Hoogteijling, L.; Antonissen, C.; Leer, R.J.; Pouwels, P.H.; Boersma, W.J.A.; Shaw, D.M.

    1999-01-01

    Lactobacillus strains possess properties that make them attractive candidates as vehicles for oral administration of therapeutics. In this report we describe the construction and analysis of recombinant Lactobacillus casei applicable in oral vaccination against an infectious disease (tetanus) and in

  7. Human dendritic cell DC-SIGN and TLR-2 mediate complementary immune regulatory activities in response to Lactobacillus rhamnosus JB-1.

    Science.gov (United States)

    Konieczna, Patrycja; Schiavi, Elisa; Ziegler, Mario; Groeger, David; Healy, Selena; Grant, Ray; O'Mahony, Liam

    2015-01-01

    The microbiota is required for optimal host development and ongoing immune homeostasis. Lactobacilli are common inhabitants of the mammalian large intestine and immunoregulatory effects have been described for certain, but not all, strains. The mechanisms underpinning these protective effects are beginning to be elucidated. One such protective organism is Lactobacillus rhamnosus JB-1 (Lb. rhamnosus JB-1). Lb. murinus has no such anti-inflammatory protective effects and was used as a comparator organism. Human monocyte-derived dendritic cells (MDDCs) were co-incubated with bacteria and analysed over time for bacterial adhesion and intracellular processing, costimulatory molecule expression, cytokine secretion and induction of lymphocyte polarization. Neutralising antibodies were utilized to identify the responsible MDDC receptors. Lb. rhamnosus JB-1 adhered to MDDCs, but internalization and intracellular processing was significantly delayed, compared to Lb. murinus which was rapidly internalized and processed. Lb. murinus induced CD80 and CD86 expression, accompanied by high levels of cytokine secretion, while Lb. rhamnosus JB-1 was a poor inducer of costimulatory molecule expression and cytokine secretion. Lb. rhamnosus JB-1 primed MDDCs induced Foxp3 expression in autologous lymphocytes, while Lb. murinus primed MDDCs induced Foxp3, T-bet and Ror-γt expression. DC-SIGN was required for Lb. rhamnosus JB-1 adhesion and influenced IL-12 secretion, while TLR-2 influenced IL-10 and IL-12 secretion. Here we demonstrate that the delayed kinetics of bacterial processing by MDDCs correlates with MDDC activation and stimulation of lymphocytes. Thus, inhibition or delay of intracellular processing may be a novel strategy by which certain commensals may avoid the induction of proinflammatory responses.

  8. Human dendritic cell DC-SIGN and TLR-2 mediate complementary immune regulatory activities in response to Lactobacillus rhamnosus JB-1.

    Directory of Open Access Journals (Sweden)

    Patrycja Konieczna

    Full Text Available The microbiota is required for optimal host development and ongoing immune homeostasis. Lactobacilli are common inhabitants of the mammalian large intestine and immunoregulatory effects have been described for certain, but not all, strains. The mechanisms underpinning these protective effects are beginning to be elucidated. One such protective organism is Lactobacillus rhamnosus JB-1 (Lb. rhamnosus JB-1. Lb. murinus has no such anti-inflammatory protective effects and was used as a comparator organism. Human monocyte-derived dendritic cells (MDDCs were co-incubated with bacteria and analysed over time for bacterial adhesion and intracellular processing, costimulatory molecule expression, cytokine secretion and induction of lymphocyte polarization. Neutralising antibodies were utilized to identify the responsible MDDC receptors. Lb. rhamnosus JB-1 adhered to MDDCs, but internalization and intracellular processing was significantly delayed, compared to Lb. murinus which was rapidly internalized and processed. Lb. murinus induced CD80 and CD86 expression, accompanied by high levels of cytokine secretion, while Lb. rhamnosus JB-1 was a poor inducer of costimulatory molecule expression and cytokine secretion. Lb. rhamnosus JB-1 primed MDDCs induced Foxp3 expression in autologous lymphocytes, while Lb. murinus primed MDDCs induced Foxp3, T-bet and Ror-γt expression. DC-SIGN was required for Lb. rhamnosus JB-1 adhesion and influenced IL-12 secretion, while TLR-2 influenced IL-10 and IL-12 secretion. Here we demonstrate that the delayed kinetics of bacterial processing by MDDCs correlates with MDDC activation and stimulation of lymphocytes. Thus, inhibition or delay of intracellular processing may be a novel strategy by which certain commensals may avoid the induction of proinflammatory responses.

  9. The Effect of Lactulose on the Survival of Lactobacillus rhamnosus in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and in vivo

    OpenAIRE

    Kontula, Pia; Nollet, Lode; Saarela, Maria; Vilpponen-Salmela, Terttu; Verstraete, Willy; Mattila-Sandholm, Tiina; von Wright, Atte

    2011-01-01

    The effect of lactulose on the survival of Lactobacillus rhamnosus VTT E-97800 and on the colon microbiota and its metabolic activity was studied using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) model. L. rhamnosus VTT E-97800 and lactulose together enhanced the production of butyric acid and decreased ammonium concentration in the model. The numbers of Bacteroides were observed to decrease 2.5–2.0 log10 cfu:ml during the SHIME experiment. In the subsequent in viv...

  10. Identification of lactobacillus casei-BDI and Streptococcus thermophilus by fluorescence spectra

    Institute of Scientific and Technical Information of China (English)

    Hao Wu; Tuo Zhu; Wei Chen; Hao Zhang; Jianlei Yang

    2009-01-01

    Fluorescence analysis applied in the study of lactic acid bacteria (LAB) provides a new method and theory to study probiotics and realize the detection and identification of the strains.It is also possible to achieve automation and computerization.In this letter,the differences between the fluorescence spectra of lactobacillus casei-BDI (Lc-BDI) and Streptococcus thermophilus (St) are shown,and the second-order derivative spectra are used to further study the diversity of these two strains.According to the results,with thc excitation wavelengths of 285 and 340 nm,there are significant differences between them.The experiment is repeated for 6 times,showing good repetitiveness.

  11. Epithelial cell proliferation arrest induced by lactate and acetate from Lactobacillus casei and Bifidobacterium breve.

    Directory of Open Access Journals (Sweden)

    Takahiro Matsuki

    Full Text Available In an attempt to identify and characterize how symbiotic bacteria of the gut microbiota affect the molecular and cellular mechanisms of epithelial homeostasis, intestinal epithelial cells were co-cultured with either Lactobacillus or Bifidobacterium as bona fide symbionts to examine potential gene modulations. In addition to genes involved in the innate immune response, genes encoding check-point molecules controlling the cell cycle were among the most modulated in the course of these interactions. In the m-ICcl2 murine cell line, genes encoding cyclin E1 and cyclin D1 were strongly down regulated by L. casei and B. breve respectively. Cell proliferation arrest was accordingly confirmed. Short chain fatty acids (SCFA were the effectors of this modulation, alone or in conjunction with the acidic pH they generated. These results demonstrate that the production of SCFAs, a characteristic of these symbiotic microorganisms, is potentially an essential regulatory effector of epithelial proliferation in the gut.

  12. Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Yasaman Tavakoli

    2015-09-01

    Full Text Available Gamma-amino butyric acid (GABA possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad gene of a local strains Lactobacillus casei was identified and cloned. In order to clone the gad gene from this strain, the PCR was carried out using primers designed based on conserved regions. The PCR product was purified and ligated into PGEM-T vector. Comparison of obtained sequences shows that this fragment codes the pyridoxal 5′-phosphate binding region. This strain could possibly be used for the industrial GABA production and also for development of functional fermented foods. Gad gene manipulation can also either decrease or increase the activity of enzyme in bacteria.

  13. EFFECT OF CULTURE MEDIUM ON BACTERIOCIN PRODUCTION BY LACTOBACILLUS RHAMNOSUS HN001 AND LACTOBACILLUS REUTERI ATCC 53608

    OpenAIRE

    Aguilar-Uscanga B. R.; Solís-Pacheco J. R.; Plascencia L.; Aguilar-Uscanga M. G.; García H. S.; Lacroix M.

    2013-01-01

    The aim of this study was to evaluate the effect of media on bacteriocin production by Lactobacillus rhamnosus HN001 and Lactobacillus reuteri ATCC 53608 using three different media: YPM, YPF and MRS supplemented with glucose and K2HPO4. The optimum temperature was 37°C and initial pH 6.5. Bacteriocin-like substances produced by tested bacteria in MRS medium supplemented with glucose and K2HPO4 exhibited a broad antimicrobial spectrum determined by well diffusion assay against indicator bacte...

  14. An l-Fucose Operon in the Probiotic Lactobacillus rhamnosus GG Is Involved in Adaptation to Gastrointestinal Conditions

    OpenAIRE

    Becerra, Jimmy E.; Yebra, María J; Monedero, Vicente

    2015-01-01

    l-Fucose is a sugar present in human secretions as part of human milk oligosaccharides, mucins, and other glycoconjugates in the intestinal epithelium. The genome of the probiotic Lactobacillus rhamnosus GG (LGG) carries a gene cluster encoding a putative l-fucose permease (fucP), l-fucose catabolic pathway (fucI, fucK, fucU, and fucA), and a transcriptional regulator (fucR). The metabolism of l-fucose in LGG results in 1,2-propanediol production, and their fucI and fucP mutants displayed a s...

  15. Asthma Prevention by Lactobacillus Rhamnosus in a Mouse Model is Associated With CD4+CD25+Foxp3+ T Cells

    OpenAIRE

    Jang, Seong-Ok; Kim, Ha-Jung; KIM, YOUNG-JOON; Kang, Mi-Jin; Kwon, Ji-Won; Seo, Ju-Hee; Kim, Hyung Young; Kim, Byoung-Ju; Yu, Jinho; Hong, Soo-Jong

    2012-01-01

    Purpose Probiotic bacteria can induce immune regulation or immune tolerance in allergic diseases. The underlying mechanisms have been recently investigated, but are still unclear. The aim of this study was to evaluate the protective effects of the probiotic Lactobacillus rhamnosus (Lcr35) in a mouse model of asthma and to identify its mechanism of action. Methods Lcr35 was administered daily by the oral route at a dosage of 1×109 CFU/mouse in BALB/c mice for 7 days before the first sensitizat...

  16. Lactobacillus rhamnosus GG reduces hepatic TNFα production and inflammation in chronic alcohol-induced liver injury.

    Science.gov (United States)

    Wang, Yuhua; Liu, Yanlong; Kirpich, Irina; Ma, Zhenhua; Wang, Cuiling; Zhang, Min; Suttles, Jill; McClain, Craig; Feng, Wenke

    2013-09-01

    The therapeutic effects of probiotic treatment in alcoholic liver disease (ALD) have been studied in both patients and experimental animal models. Although the precise mechanisms of the pathogenesis of ALD are not fully understood, gut-derived endotoxin has been postulated to play a crucial role in hepatic inflammation. Previous studies have demonstrated that probiotic therapy reduces circulating endotoxin derived from intestinal gram-negative bacteria in ALD. In this study, we investigated the effects of probiotics on hepatic tumor necrosis factor-α (TNFα) production and inflammation in response to chronic alcohol ingestion. Mice were fed Lieber DeCarli liquid diet containing 5% alcohol for 8weeks, and Lactobacillus rhamnosus GG (LGG) was supplemented in the last 2 weeks. Eight-week alcohol feeding caused a significant increase in hepatic inflammation as shown by histological assessment and hepatic tissue myeloperoxidase activity assay. Two weeks of LGG supplementation reduced hepatic inflammation and liver injury and markedly reduced TNFα expression. Alcohol feeding increased hepatic mRNA expression of Toll-like receptors (TLRs) and CYP2E1 and decreased nuclear factor erythroid 2-related factor 2 expression. LGG supplementation attenuated these changes. Using human peripheral blood monocytes-derived macrophages, we also demonstrated that incubation with ethanol primes both lipopolysaccharide- and flagellin-induced TNFα production, and LGG culture supernatant reduced this induction in a dose-dependent manner. In addition, LGG treatment also significantly decreased alcohol-induced phosphorylation of p38 MAP kinase. In conclusion, probiotic LGG treatment reduced alcohol-induced hepatic inflammation by attenuation of TNFα production via inhibition of TLR4- and TLR5-mediated endotoxin activation. PMID:23618528

  17. Preserving viability of Lactobacillus rhamnosus GG in vitro and in vivo by a new encapsulation system.

    Science.gov (United States)

    Li, Ran; Zhang, Yufeng; Polk, D Brent; Tomasula, Peggy M; Yan, Fang; Liu, LinShu

    2016-05-28

    Probiotics have shown beneficial effects on health and prevention of diseases in humans. However, a concern for application of probiotics is the loss of viability during storage and gastrointestinal transit. The aim of this study was to develop an encapsulation system to preserve viability of probiotics when they are administrated orally and apply Lactobacillus rhamnosus GG (LGG) as a probiotic model to evaluate the effectiveness of this approach using in vitro and in vivo experiments. LGG was encapsulated in hydrogel beads prepared using pectin, a food grade polysaccharide, glucose, and calcium chloride, and lyophilized by freeze-drying. Encapsulated LGG was cultured in vitro under the condition that mimicked the physiological environment of the human gastrointestinal tract. Compared to non-encapsulated LGG, encapsulation increased tolerance of LGG in the acid condition, protected LGG from protease digestion, and improved shelf time when stored at the ambient condition, in regard of survivability and production of p40, a known LGG-derived protein involved in LGG's beneficial effects on intestinal homeostasis. To evaluate the effects of encapsulation on p40 production in vivo and prevention of intestinal inflammation by LGG, mice were gavaged with LGG containing beads and treated with dextran sulphate sodium (DSS) to induce intestinal injury and colitis. Compared to non-encapsulated LGG, encapsulated LGG enhanced more p40 production in mice, and exerted higher levels of effects on prevention of DSS-induced colonic injury and colitis and suppression of pro-inflammatory cytokine production. These data indicated that the encapsulation system developed in this study preserves viability of LGG in vitro and in vivo, leading to longer shelf time and enhancing the functions of LGG in the gastrointestinal tract. Thus, this encapsulation approach may have the potential application for improving efficacy of probiotics.

  18. Preserving viability of Lactobacillus rhamnosus GG in vitro and in vivo by a new encapsulation system

    Science.gov (United States)

    Li, Ran; Zhang, Yufeng; Polk, D. Brent; Tomasula, Peggy M.; Yan, Fang; Liu, LinShu

    2016-01-01

    Probiotics have shown beneficial effects on health and prevention of diseases in humans. However, a concern for application of probiotics is the loss of viability during storage and gastrointestinal transit. The aim of this study was to develop an encapsulation system to preserve viability of probiotics when they are administrated orally and apply Lactobacillus rhamnosus GG (LGG) as a probiotic model to evaluate the effectiveness of this approach using in vitro and in vivo experiments. LGG was encapsulated in hydrogel beads prepared using pectin, a food grade polysaccharide, glucose, and calcium chloride, and lyophilized by freeze-drying. Encapsulated LGG was cultured in vitro under the condition that mimicked the physiological environment of the human gastrointestinal tract. Compared to non-encapsulated LGG, encapsulation increased tolerance of LGG in the acid condition, protected LGG from protease digestion, and improved shelf time when stored at the ambient condition, in regard of survivability and production of p40, a known LGG-derived protein involved in LGG’s beneficial effects on intestinal homeostasis. To evaluate the effects of encapsulation on p40 production in vivo and prevention of intestinal inflammation by LGG, mice were gavaged with LGG containing beads and treated with dextran sulphate sodium (DSS) to induce intestinal injury and colitis. Compared to non-encapsulated LGG, encapsulated LGG enhanced more p40 production in mice, and exerted higher levels of effects on prevention of DSS-induced colonic injury and colitis and suppression of pro-inflammatory cytokine production. These data indicated that the encapsulation system developed in this study preserves viability of LGG in vitro and in vivo, leading to longer shelf time and enhancing the functions of LGG in the gastrointestinal tract. Thus, this encapsulation approach may have the potential application for improving efficacy of probiotics. PMID:27063422

  19. Immunomodulatory consequences of oral administration of Lactobacillus rhamnosus strain GG in healthy volunteers.

    Science.gov (United States)

    Schultz, Michael; Linde, Hans-Jörg; Lehn, Norbert; Zimmermann, Kurt; Grossmann, Johannes; Falk, Werner; Schölmerich, Jürgen

    2003-05-01

    Probiotic microorganisms, especially lactic acid bacteria, are effective in the treatment of infectious diarrhoeal diseases and experimental colitis. Although the mechanisms by which these organisms exert their anti-inflammatory effects are largely unknown, immunomodulating effects are suggested. The objective of this study was to examine the effect of a 5-week oral administration of Lactobacillus rhamnosus subspecies GG (Lb. GG) on the cellular immune response to intestinal microorganisms in ten healthy volunteers. Peripheral blood cells (PB) were stimulated with either 'self' or 'non-self' preparations of faecal samples and isolated Bacteroides fragilis group-organisms (Bfg) or Escherichia coli (Esch. coli), and pro- and anti-inflammatory cytokines (IL-10, IL-4, IL-6, IFN-gamma, TNF-alpha) were measured in the culture supernatant. CD4+ T-lymphocyte activation was determined by measurement of intracellular ATP following lysis of the cells. The activational response of CD4+ T-lymphocytes towards isolated and heat-inactivated intestinal organisms was increased after the probiotic treatment. Additionally, TNF-alpha, IL-6 and in part IFN-gamma cytokine secretion by PB cells following stimulation with whole stool preparations and single members of the flora was significantly decreased, whereas the IL-10 and in part IL-4 cytokine secretion was increased at the end of the study. In contrast, the activational response of CD4+ T-lymphocytes following stimulation with whole 'non-self' intestinal flora was higher than by 'self' intestinal flora, but both responses showed a trend towards a reduction at the end of the study. This study documents a direct effect by Lb. GG on the cellular immune system of healthy volunteers and offers a promising tool to investigate systemic immunomodulation due to oral administration of probiotic microorganisms.

  20. Preserving viability of Lactobacillus rhamnosus GG in vitro and in vivo by a new encapsulation system.

    Science.gov (United States)

    Li, Ran; Zhang, Yufeng; Polk, D Brent; Tomasula, Peggy M; Yan, Fang; Liu, LinShu

    2016-05-28

    Probiotics have shown beneficial effects on health and prevention of diseases in humans. However, a concern for application of probiotics is the loss of viability during storage and gastrointestinal transit. The aim of this study was to develop an encapsulation system to preserve viability of probiotics when they are administrated orally and apply Lactobacillus rhamnosus GG (LGG) as a probiotic model to evaluate the effectiveness of this approach using in vitro and in vivo experiments. LGG was encapsulated in hydrogel beads prepared using pectin, a food grade polysaccharide, glucose, and calcium chloride, and lyophilized by freeze-drying. Encapsulated LGG was cultured in vitro under the condition that mimicked the physiological environment of the human gastrointestinal tract. Compared to non-encapsulated LGG, encapsulation increased tolerance of LGG in the acid condition, protected LGG from protease digestion, and improved shelf time when stored at the ambient condition, in regard of survivability and production of p40, a known LGG-derived protein involved in LGG's beneficial effects on intestinal homeostasis. To evaluate the effects of encapsulation on p40 production in vivo and prevention of intestinal inflammation by LGG, mice were gavaged with LGG containing beads and treated with dextran sulphate sodium (DSS) to induce intestinal injury and colitis. Compared to non-encapsulated LGG, encapsulated LGG enhanced more p40 production in mice, and exerted higher levels of effects on prevention of DSS-induced colonic injury and colitis and suppression of pro-inflammatory cytokine production. These data indicated that the encapsulation system developed in this study preserves viability of LGG in vitro and in vivo, leading to longer shelf time and enhancing the functions of LGG in the gastrointestinal tract. Thus, this encapsulation approach may have the potential application for improving efficacy of probiotics. PMID:27063422

  1. Characterization of pMC11, a plasmid with dual origins of replication isolated from Lactobacillus casei MCJ and construction of shuttle vectors with each replicon

    DEFF Research Database (Denmark)

    Chen, Zhengjun; Lin, Jinzhong; Ma, Chengjie;

    2014-01-01

    . These plasmids showed distinct properties: pEL5.7 was capable of replicating in L. casei MCJΔ1 and Lactobacillus delbrueckii subsp. lactic LBCH-1 but failed to do so in two other tested lactobacilli strains whereas pEL5.6 replicated in three different strains, including L. casei MCJΔ1, L. casei NJ, Lactobacillus...... cloning and heterologous gene expression in lactobacilli....

  2. Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis(syn. Brassica campestris ssp. pekinensis)

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiao-wei; XIE Cong-hua; WANG Xiao-wu; WU Jian; ZHAO Jian-jun; SONG Xiao-fei; LI Ying; ZHANG Yan-guo; XU Dong-hui; SUN Ri-fei; YUAN Yu-xiang

    2006-01-01

    A genetic linkage map of Brassica rapa ssp. pekinensis was constructed with 186 AFLP (amplified fragment length polymorphism) markers by using a doubled-haploid (DH) population with 183 individuals. The individuals were derived from F1 which was developed by crossing a bolting resistant DH line Y-177-12 and an easy bolting DH line Y195-93a.AFLPs were generated by the use of restriction enzymes EcoR Ⅰ and Mse Ⅰ. The segregation of each marker and linkage was analyzed by using JoinMap version 3.0. Mapped markers were aligned in ten linkage groups which covered 887.8 cM with an average marker interval of 4.47 cM. Markers showing skewed segregation ratio were clustered in six LGs.Quantitative trait loci (QTL) were mapped for bolting resistance by using MAPQTL 4.0 package. Four QTLs explaining from 7.0 to 9.4% of the total variation were detected, all of them increase bolting resistance. These mapped QTLs could be used to develop a marker assisted selection programme for bolting resistance breeding.

  3. Stability of Lactobacillus casei Zhang during subculturing in carbon source limited media for 1 000 generations%碳源限制条件下Lactobacillus casei Zhang 传1000代过程中的稳定性

    Institute of Scientific and Technical Information of China (English)

    张文羿; 白梅; 张和平

    2014-01-01

    通过检测细胞形态、活菌数、浊度和菌株活力的变化情况对Lactobacillus casei Zhang在碳源限制性培养基中连续传代1 000代期间稳定性进行了初步研究.结果表明:L.casei Zhang在传代期间细胞形态有微弱变化;活菌数、浊度和菌株活力总体基本维持不变;Lcasei Zhang在碳源限制条件下长期传代过程中的稳定性可能受多种因素影响.

  4. Study on the improvement of heat resistance of Lactobacillus casei BD- Ⅱ by heat shock treatment%利用热激处理提高益生菌Lactobacillus casei BD-Ⅱ抗热性的研究

    Institute of Scientific and Technical Information of China (English)

    陆英; 陈卫; 田丰伟; 赵建新; 张灏

    2006-01-01

    对酪乳杆菌Lactobacillus casei BD-Ⅱ的抗热性以及不同热激条件对抗热性的影响进行了研究.菌株细胞在灭菌脱脂乳中70,72,75℃时的D值分别为36.2,26.5和11.3 s.通过正交实验确定L. casei BD-Ⅱ最适热激条件:菌龄7 h的细胞在热激温度50℃下处理45 min.在此条件下60℃(90 s)处理存活率为0.146%,较未处理细胞提高2.11倍.

  5. 干酪乳杆菌Lactobacillus casei 393在鼠消化道内定植能力及分布规律的研究%Colonization ability and distribution of Lactobacillus casei 393 in mouse gut

    Institute of Scientific and Technical Information of China (English)

    徐义刚; 崔丽春; 唐丽杰; 赵丽丽; 李一经

    2007-01-01

    研究了干酪乳杆菌Lactobadllus casei 393在小鼠肠道中定植能力及分布规律.利用羧基荧光素二乙酸琥珀酰亚胺酯分子探针对干酪乳杆菌Lactobacillus casei 393进行标记,以每只1010 mL-1的量,口服途径喂给BALB/c鼠,分别于口服后的第1,3,5,6 d取其十二指肠、空肠、回肠、结肠等肠段,通过流式细胞仪检测肠内的标记阳性菌.实验结果显示:经口服后第5 d,干酪乳杆菌Lactobacillus casei 393开始在BALB/c鼠肠道中定植,在十二指肠、空肠、回肠、结肠的定植率分别为28.70%,29.77%,15.63%,26.17%,第6 d标记的阳性茵出现明显的生长趋势,阳性检出率分别为71.77%,57.31%,65.44%,53.13%.研究表明干酪乳杆菌Lactobacillus casei 393具有良好的肠道定植能力.

  6. The Effect of Glucose on the Performance ofLactobacillus casei KDL22 during Fermentation and Storage Process%葡萄糖对Lactobacillus caseiKDL22发酵及贮藏性能的影响

    Institute of Scientific and Technical Information of China (English)

    吴可非; 马雨璇; 桑跃; 葛绍阳; 刘松玲; 张清海; 刘治麟; 赵亮

    2016-01-01

    干酪乳杆菌(Lactobacilus casei)是目前研究深入、应用广泛的益生菌菌种,优良的发酵及贮藏性能是干酪乳杆菌成功应用的重要条件。为了提高Lactobacilus casei KDL22(KDL22)的应用性能,在发酵及贮藏过程中通过添加葡萄糖来提高菌株的发酵及贮藏性能。结果表明,与商业菌株L.casei shirota(LCS)相比,KDL22表现了较好的发酵性能,但贮藏性能较差;添加葡萄糖可以增加KDL22发酵过程产酸性能及活菌数。为KDL22的有效应用提供了理论基础,同时也为提高益生菌的应用性能提供了新的思路。%Lactobacilus casei is one of the most widely recognized probiotic bacteria,and its excelent performance during pro-duction and storage process is important for application. In order to improve the ability ofLactobacilus casei KDL22,glucose was added during fermentation and storage process,and the bacterial counts and acidity were detected. The results showed that the addition of glucose indeed elevated the bacterial counts and acidity during fermentation and storage process. This study provided the new idea for the improvement of the performance for the probiotic during its application.

  7. Versatile modes of peptide recognition by the AAA+ adaptor protein SspB

    Energy Technology Data Exchange (ETDEWEB)

    Levchenko, Igor; Grant, Robert A.; Flynn, Julia M.; Sauer, Robert T.; Baker, Tania A. (MIT)

    2010-07-19

    Energy-dependent proteases often rely on adaptor proteins to modulate substrate recognition. The SspB adaptor binds peptide sequences in the stress-response regulator RseA and in ssrA-tagged proteins and delivers these molecules to the AAA+ ClpXP protease for degradation. The structure of SspB bound to an ssrA peptide is known. Here, we report the crystal structure of a complex between SspB and its recognition peptide in RseA. Notably, the RseA sequence is positioned in the peptide-binding groove of SspB in a direction opposite to the ssrA peptide, the two peptides share only one common interaction with the adaptor, and the RseA interaction site is substantially larger than the overlapping ssrA site. This marked diversity in SspB recognition of different target proteins indicates that it is capable of highly flexible and dynamic substrate delivery.

  8. Staphylococcus saprophyticus surface-associated protein (Ssp) is associated with lifespan reduction in Caenorhabditis elegans.

    Science.gov (United States)

    Szabados, Florian; Mohner, Amelie; Kleine, Britta; Gatermann, Sören G

    2013-10-01

    Staphylococcal lipases have been proposed as pathogenicity factors. In Staphylococcus saprophyticus the surface-associated protein (Ssp) has been previously characterized as a cell wall-associated true lipase. A S. saprophyticus Δssp::ermB mutant has been described as less virulent in an in vivo model of urinary tract infection compared with its wild-type. This is the first report showing that S. saprophyticus induced a lifespan reduction in Caenorhabditis elegans similar to that of S. aureus RN4220. In two S. saprophyticus Δssp::ermB mutants lifespan reduction in C. elegans was partly abolished. In order to attribute virulence to the lipase activity itself and distinguish this phenomenon from the presence of the Ssp-protein, the conserved active site of the lipase was modified by site-directed ligase-independent mutagenesis and lipase activity-deficient mutants were constructed. These results indicate that the Ssp is associated with pathogenicity in C. elegans and one could speculate that the lipase activity itself is responsible for this virulence.

  9. A modified PCR-SSP method for the identification of ABO blood group antigens.

    Science.gov (United States)

    Downing, J; Darke, C

    2003-08-01

    The ABO blood group antigens are carbohydrate molecules synthesized by the glycosyltransferases encoded by the ABO gene on chromosome 9. Kidney transplantation across the ABO barrier generally leads to rapid humoral graft rejection due to the presence of naturally occurring antibodies to the A and B antigens. We have developed a method for ABO typing our cadaveric organ donors by the polymerase chain reaction using sequence-specific primers (PCR-SSP). The method uses 12 primers in eight PCR mixtures and is performed under the same conditions as our routine HLA-A, B, C PCR-SSP typing. The PCR-SSP-based types of 166 regular blood donors and 148 cadaveric organ donors all showed total concordance with their serologically assigned ABO groups. Six individuals possessing the ABO A subgroups (A3, Ax and Aend) all typed as A1 by PCR-SSP, as expected. PCR-SSP is an appropriate method for ABO typing of cadaveric organ donors and, importantly, enables both ABO and HLA typing to be performed on the same DNA material.

  10. Cytogenetic Diversity of Simple Sequences Repeats in Morphotypes of Brassica rapa ssp. chinensis.

    Science.gov (United States)

    Zheng, Jin-Shuang; Sun, Cheng-Zhen; Zhang, Shu-Ning; Hou, Xi-Lin; Bonnema, Guusje

    2016-01-01

    A significant fraction of the nuclear DNA of all eukaryotes is comprised of simple sequence repeats (SSRs). Although these sequences are widely used for studying genetic variation, linkage mapping and evolution, little attention had been paid to the chromosomal distribution and cytogenetic diversity of these sequences. In this paper, we report the distribution characterization of mono-, di-, and tri-nucleotide SSRs in Brassica rapa ssp. chinensis. Fluorescence in situ hybridization was used to characterize the cytogenetic diversity of SSRs among morphotypes of B. rapa ssp. chinensis. The proportion of different SSR motifs varied among morphotypes of B. rapa ssp. chinensis, with tri-nucleotide SSRs being more prevalent in the genome of B. rapa ssp. chinensis. We determined the chromosomal locations of mono-, di-, and tri-nucleotide repeat loci. The results showed that the chromosomal distribution of SSRs in the different morphotypes is non-random and motif-dependent, and allowed us to characterize the relative variability in terms of SSR numbers and similar chromosomal distributions in centromeric/peri-centromeric heterochromatin. The differences between SSR repeats with respect to abundance and distribution indicate that SSRs are a driving force in the genomic evolution of B. rapa species. Our results provide a comprehensive view of the SSR sequence distribution and evolution for comparison among morphotypes B. rapa ssp. chinensis. PMID:27507974

  11. A Brief Report on the Study of Lactobacillus casei Zhang%益生菌Lactobacillus casei Zhang的研究简况

    Institute of Scientific and Technical Information of China (English)

    赫荣乔

    2010-01-01

    @@ 用于发酵乳制品生产的益生乳酸菌菌种和发酵剂的研制对于我国相关产业的生存与发展至关重要.为了建立具有我国自主知识产权的益生乳酸菌菌种和发酵剂的相关技术,张和平等人分离并系统研究了一株益生乳酸菌,命名为Lactobacillus casei Zhang[1].目前,该项目已进入益生乳酸菌高密度发酵中试和规模生产的研究阶段.据该研究组报道,其冷冻干燥菌体干粉的平均活菌数达到2 x 1011 CFU/g.他们对该益生乳酸菌进行了全基因组测定,开展了蛋白质组、细胞生长以及代谢机制的研究.发表在本刊的一文就是他们工作的一部分[2],文章介绍了所筛选益生菌的生物学特性和营养需求,对其培养基成分和含量进行了筛选和优化,以确定适宜的增殖培养基,用于菌体的高密度培养,为高浓缩益生菌制剂的制备和生产创造了条件.

  12. Production of dry Lactobacillus rhamnosus GG preparations by spray drying and lyophilization in aqueous two-phase systems

    Directory of Open Access Journals (Sweden)

    Katarzyna Leja

    2009-12-01

    Full Text Available Background. Drying is the oldest method of food preservation. It works by removing water from the food, which prevents the growth of microorganisms and decay. Moreover, spray or freeze drying is also used for the preservation of probiotic cultures. The aim of this study was to compare a survival rate of probiotic bacteria Lactobacillus rhamnosus during spray and freeze drying in ATPS. These results were also compared with survival rate of cells dried under the same conditions but suspended only in skim milk, 6% solution of PVP or 6% solution of dextran. Material and methods. The bacteria Lactobacillus rhamnosus GGwere suspended and spray or freeze dried in various types of aqueous two-phase emulsions: PVP/dextran, PEG4000/dextran and PEG8000/dextran. These emulsions consisted of different types of polymers and had varying ratio of polymers in dispersed (dextran and dispersing (PEG and PVP phases. Results. The research demonstrated that survival rate of bacteria directly after drying depended mainly on protective reagent, rather than on drying method. After 30-day-storage of the dried bacteria cell specimens, the highest survival rate was noted in case of freeze dried cells in milk. In case of spray drying the highest cell survival rate was observed when emulsion PVP3.6%/dextran2.4% was used as a drying medium. Conclusions. Finally, it has been found that cell survival rate was not strongly influenced by the storage temperature of the powder but it depended on the drying medium.  

  13. Lactobacillus rhamnosus GG Intake Modifies Preschool Children's Intestinal Microbiota, Alleviates Penicillin-Associated Changes, and Reduces Antibiotic Use.

    Directory of Open Access Journals (Sweden)

    Katri Korpela

    Full Text Available Antibiotic use is considered among the most severe causes of disturbance to children's developing intestinal microbiota, and frequently causes adverse gastrointestinal effects ranging from mild and transient diarrhoea to life-threatening infections. Probiotics are commonly advocated to help in preventing antibiotic-associated gastrointestinal symptoms. However, it is currently unknown whether probiotics alleviate the antibiotic-associated changes in children's microbiota. Furthermore, it is not known how long-term probiotic consumption influences the developing microbiota of children. We analysed the influence of long-term Lactobacillus rhamnosus GG intake on preschool children's antibiotic use, and antibiotic-associated gastrointestinal complaints in a double blind, randomized placebo-controlled trial with 231 children aged 2-7. In addition, we analysed the effect of L. rhanmosus GG on the intestinal microbiota in a subset of 88 children. The results show that long-term L. rhamnosus GG supplementation has an influence on the composition of the intestinal microbiota in children, causing an increase in the abundance of Prevotella, Lactococcus, and Ruminococcus, and a decrease in Escherichia. The treatment appeared to prevent some of the changes in the microbiota associated with penicillin use, but not those associated with macrolide use. The treatment, however, did reduce the frequency of gastrointestinal complaints after a macrolide course. Finally, the treatment appeared to prevent certain bacterial infections for up to 3 years after the trial, as indicated by reduced antibiotic use.ClinicalTrials.gov NCT01014676.

  14. Rhizospheric Bacterial Strain Brevibacterium casei MH8a Colonizes Plant Tissues and Enhances Cd, Zn, Cu Phytoextraction by White Mustard

    OpenAIRE

    Płociniczak, Tomasz; Sinkkonen, Aki; Romantschuk, Martin; Sułowicz, Sławomir; Piotrowska-Seget, Zofia

    2016-01-01

    Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants. The aim of this study was to determine whether the multiple-tolerant bacterium, Brevibacterium casei MH8a isolated from the heavy metal-contaminated rhizosphere soil of Sinapis alba L., is able to promote plant growth and enhance Cd, Zn, and Cu uptake ...

  15. 益生菌Lactobacillus casei Zhang全基因组图谱绘制完成

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    由内蒙古农业大学“乳品生物技术与工程”教育部重点实验室主持进行的益生菌L.casei Zhang的全基因组序列测定于2008年5月18日全部完成。这是我国第一个完成的乳酸菌基因全序列测定。

  16. Utilization of Natural Fucosylated Oligosaccharides by Three Novel α-l-Fucosidases from a Probiotic Lactobacillus casei Strain ▿

    Science.gov (United States)

    Rodríguez-Díaz, Jesús; Monedero, Vicente; Yebra, María J.

    2011-01-01

    Three putative α-l-fucosidases encoded in the Lactobacillus casei BL23 genome were cloned and purified. The proteins displayed different abilities to hydrolyze natural fucosyloligosaccharides like 2′-fucosyllactose, H antigen disaccharide, H antigen type II trisaccharide, and 3′-, 4′-, and 6′-fucosyl-GlcNAc. This indicated a possible role in the utilization of oligosaccharides present in human milk and intestinal mucosa. PMID:21097595

  17. Adjuvant Effects for Oral Immunization Provided by Recombinant Lactobacillus casei Secreting Biologically Active Murine Interleukin-1β▿

    OpenAIRE

    KAJIKAWA, Akinobu; MASUDA, Kazuya; Katoh, Mitsunori; Igimi, Shizunobu

    2009-01-01

    Vaccine delivery systems using lactic acid bacteria are under development, but their efficiency is insufficient. Autologous cytokines, such as interleukin-1β (IL-1β), are potential adjuvants for mucosal vaccines and can be provided by recombinant lactic acid bacteria. The aim of this study was the construction and evaluation of recombinant Lactobacillus casei producing IL-1β as an adjuvant delivery agent. The recombinant strain was constructed using an expression/secretion vector plasmid, inc...

  18. The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG

    NARCIS (Netherlands)

    Douillard, François P.; Rasinkangas, Pia; Bhattacharjee, Arnab; Palva, Airi; Vos, De Willem M.

    2016-01-01

    Predominantly identified in pathogenic Gram-positive bacteria, sortase-dependent pili are also found in commensal species, such as the probiotic-marketed strain Lactobacillus rhamnosus strain GG. Pili are typically associated with host colonization, immune signalling and biofilm formation. Compar

  19. Acquired macrolide resistance in the human intestinal strain Lactobacillus rhamnosus E41 associated with a transition mutation in 23S rRNA genes

    OpenAIRE

    Flórez García, Ana Belén; Ladero Losada, Víctor Manuel; Álvarez Martín, Pablo; Ammor, Mohammed Salim; Álvarez González, Miguel Ángel; Mayo Pérez, Baltasar

    2007-01-01

    Restriction fragment length polymorphism and DNA sequencing of polymerase chain reaction (PCR) products showed that a Lactobacillus rhamnosus strain of human origin resistant to macrolides, from which no resistance determinants have been detected by specific PCR and microarray screening, contained a heterozygous A → G transition mutation at position 2058 (Escherichia coli numbering) of its 23S rRNA genes.

  20. Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut

    NARCIS (Netherlands)

    Ganguli, K.; Collado, M.C.; Rautava, J.; Lu, L.; Satokari, R.M.; Ossowski, von I.; Reunanen, J.; Vos, de W.M.; Palva, A.; Isolauri, E.; Salminen, S.; Walker, W.A.; Rautava, S.

    2015-01-01

    BACKGROUND: Bacterial contact in utero modulates fetal and neonatal immune responses. Maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. We investigated the immunomodulatory properties of live Lactobacillus rhamnosus GG and its SpaC pilus adhesin in human f

  1. No evidence of harms of probiotic Lactobacillus rhamnosus GG ATCC 53103 in healthy elderly-a Phase I Open Label Study to assess safety, tolerability and cytokine responses

    Science.gov (United States)

    Although Lactobacillus rhamnosus GG ATCC 53103 (LGG) has been consumed since the mid 1990s by between 2 and 5 million people daily, the scientific literature lacks rigorous clinical trials that describe the potential harms of LGG, particularly in the elderly. The primary objective of this open label...

  2. Proteome Analysis of Lactobacillus rhamnosus GG Using 2-D DIGE and Mass Spectrometry Shows Differential Protein Production in Laboratory and Industrial-Type Growth Media

    NARCIS (Netherlands)

    Koskenniemi, K.; Koponen, J.; Kankainen, M.; Savijoki, K.; Tynkkynen, S.; Vos, de W.M.; Kalkkinen, N.; Varmanen, P.

    2009-01-01

    Lactobacillus rhamnosus GG (LGG) is one of the most extensively studied and widely used probiotic bacteria. While the benefits of LGG treatment in gastrointestinal disorders and immunomodulation are well-documented, functional genomics research of this bacterium has only recently been initiated. In

  3. Extensively hydrolysed casein formula supplemented with Lactobacillus rhamnosus GG maintains hypoallergenic status : randomised double-blind, placebo-controlled crossover trial

    NARCIS (Netherlands)

    Muraro, Antonella; Hoekstra, Maarten O.; Meijer, Yolanda; Lifschitz, Carlos; Wampler, Jennifer L.; Harris, Cheryl; Scalabrin, Deolinda M. F.

    2012-01-01

    Objective: To evaluate the hypoallergenicity of an extensively hydrolysed (EH) casein formula supplemented with Lactobacillus rhamnosus GG (LGG). Design: A prospective, randomised, double-blind, placebo-controlled crossover trial. Setting: Two study sites in Italy and The Netherlands. Study particip

  4. The Influence of Probiotic Lactobacillus casei in Combination with Prebiotic Inulin on the Antioxidant Capacity of Human Plasma.

    Science.gov (United States)

    Kleniewska, Paulina; Hoffmann, Arkadiusz; Pniewska, Ewa; Pawliczak, Rafał

    2016-01-01

    The aim of the present study was to assess whether probiotic bacteria Lactobacillus casei (4 × 10(8) CFU) influences the antioxidant properties of human plasma when combined with prebiotic Inulin (400 mg). Experiments were carried out on healthy volunteers (n = 32). Volunteers were divided according to sex (16 male and 16 female) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 weeks, at the end of the study. Catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activity, and the ferric reducing ability of plasma (FRAP) in human plasma were examined. The administration of synbiotics containing L. casei plus Inulin resulted in a significant increase in FRAP values (p = 0.00008) and CAT activity (p = 0.02) and an insignificant increase in SOD and GPx activity compared to controls. Synbiotics containing L. casei (4 × 10(8) CFU) with prebiotic Inulin (400 mg) may have a positive influence on human plasma antioxidant capacity and the activity of selected antioxidant enzymes.

  5. Sonicated pineapple juice as substrate for L. casei cultivation for probiotic beverage development: process optimisation and product stability.

    Science.gov (United States)

    Costa, Mayra Garcia Maia; Fonteles, Thatyane Vidal; de Jesus, Ana Laura Tibério; Rodrigues, Sueli

    2013-08-15

    The aim of this study was to evaluate the use of sonicated pineapple juice as substrate for producing a probiotic beverage by Lactobacillus casei NRRL B442. Maximal microbial viability was found by cultivating L. casei at 31°C and pH 5.8 (optimised conditions). After fermentation, samples of sweetened and non-sweetened juice were stored. After 42 days of storage under refrigeration (4°C), the microbial viability was 6.03 Log CFU/mL in the non-sweetened sample and 4.77 Log CFU/mL in the sweetened sample. The pH of both samples decreased during storage due to lactic acid production (post acidification). The characteristic colour of the juice was maintained throughout the shelf life and no browning was observed. Sonicated pineapple juice was shown to be a suitable substrate for L. casei cultivation and for the development of an alternative non-dairy probiotic beverage. PMID:23561104

  6. Antitumoural activity of a cytotoxic peptide of Lactobacillus casei peptidoglycan and its interaction with mitochondrial-bound hexokinase.

    Science.gov (United States)

    Fichera, Giuseppe A; Fichera, Marco; Milone, Giuseppe

    2016-08-01

    In a previous study, we reported the cytotoxic activity against various tumour cells of the peptidoglycan of Lactobacillus casei. To isolate the most active components, we performed column-chromatography separation of the peptidoglycan complex and tested the related fractions for their cytotoxic activity. The most active fractions were then lyophilized and the residue was analysed by gas chromatography for its amino acid content and composition. On the basis of the known chemical formula of the basic peptidic component of the peptidoglycan complex of L. casei, a peptide was then synthesized [Europ. (CH-DE-FR-GB) Patent number 1217005; IT number 01320177] and its cytotoxicity was tested against tumoural and normal cells. The synthetic peptide was found to impair the entire metabolism of cultured tumour cells and to restore the apoptotic process. By contrast, normal cells appeared to be stimulated rather than inhibited by the peptide, whereas primary mouse embryo fibroblasts behaved similarly to tumour cells. On the basis of these results, L. casei peptidoglycan fragments and their constituent basic peptide might be applicable as potent antitumour agents. PMID:27101258

  7. The Influence of Probiotic Lactobacillus casei in Combination with Prebiotic Inulin on the Antioxidant Capacity of Human Plasma.

    Science.gov (United States)

    Kleniewska, Paulina; Hoffmann, Arkadiusz; Pniewska, Ewa; Pawliczak, Rafał

    2016-01-01

    The aim of the present study was to assess whether probiotic bacteria Lactobacillus casei (4 × 10(8) CFU) influences the antioxidant properties of human plasma when combined with prebiotic Inulin (400 mg). Experiments were carried out on healthy volunteers (n = 32). Volunteers were divided according to sex (16 male and 16 female) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 weeks, at the end of the study. Catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activity, and the ferric reducing ability of plasma (FRAP) in human plasma were examined. The administration of synbiotics containing L. casei plus Inulin resulted in a significant increase in FRAP values (p = 0.00008) and CAT activity (p = 0.02) and an insignificant increase in SOD and GPx activity compared to controls. Synbiotics containing L. casei (4 × 10(8) CFU) with prebiotic Inulin (400 mg) may have a positive influence on human plasma antioxidant capacity and the activity of selected antioxidant enzymes. PMID:27066188

  8. The transfer RNA genes in Oryza sativa L.ssp.indica

    Institute of Scientific and Technical Information of China (English)

    王希胤; 史晓黎; 郝柏林

    2002-01-01

    The availability of the draft genome sequence of Oryza sativa L. ssp. indica has made it possible to study the rice tRNA genes. A total of 596 tRNA genes, including 3 selenocysteine tRNA genes and one suppressor tRNA gene are identified in 127551 rice contigs. There are 45 species of tRNA genes and the revised wobble hypothesis proposed by Guthrie and Abelson is perfectly obeyed. The relationship between codon usage and the number of corresponding tRNA genes is discussed. Redundancy may exist in the present list of tRNA genes and novel ones may be found in the future. A set of 33 tRNA genes is discovered in the complete chloroplast genome of Oryza sativa L. ssp. indica. These tRNA genes are identical to those in ssp. japonica identified by us independently from the origional annotation.

  9. Optimal Control Strategy for Marine Ssp Podded Propulsion Motor Based on Strong Tracking-Epf

    Directory of Open Access Journals (Sweden)

    Yao Wenlong

    2015-09-01

    Full Text Available Aiming at the non-linearity of state equation and observation equation of SSP (Siemen Schottel Propulsor propulsion motor, an improved particle filter algorithm based on strong tracking extent Kalman filter (ST-EKF was presented, and it was imported into the marine SSP propulsion motor control system. The strong tracking filter was used to update particles in the new algorithm and produce importance densities. As a result, the problems of particle degeneracy and sample impoverishment were ameliorated, the propulsion motor states and the rotor resistance were estimated simultaneously using strong track filter (STF, and the tracking ability of marine SSP propulsion motor control system was improved. Simulation result shown that the improved EPF algorithm was not only improving the prediction accuracy of the motor states and the rotor resistance, but also it can satisfy the requirement of navigation in harbor. It had the better accuracy than EPF algorithm.

  10. VIABILIDADE DA ADIÇÃO DE LACTOBACILLUS CASEI (LC-1 PROTEGIDO COM TREALOSE E GOMA ACÁCIA EM SORVETES

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    Jean Clovis Bertuol de SOUZA

    2011-04-01

    Full Text Available Os probióticos têm sido incorporados em sorvetes fermentados ou não, os quais são bons veículos para fornecer esses micro-organismos na dieta humana. O presente trabalho teve como objetivo analisar a viabilidade do micro-organismo probiótico Lactobacillus casei (LC-1 adicionado na forma livre ou protegido com trealose e goma acácia em sorvete durante 98 dias de armazenamento a -20°C, bem como avaliar a aceitabilidade do ponto de vista sensorial e as características físico-químicas desse produto. A sobrevivência do L. casei controle (sem prote- ção celular e do L. casei protegido com trealose e goma acácia foi monitorada durante 98 dias de estocagem do produto a -20℃. No início do armazenamento, foi observada população média de 9,20log UFC.g-1 para o L. casei livre e 9,80log UFC.g-1 para o L. casei protegido. Porém, após 98 dias de armazenamento a -20°C, essas populações diminuíram para 6,90 e 8,14log UFC.g-1, respectivamente. A adição do L. casei livre ou protegido não afetou as características sensoriais do sorvete. A proteção celular com trealose e goma acácia em L. casei melhorou a viabilidade desse micro-organismo quando adicionado em sorvete.

  11. Genetic Interactions among AMPK Catalytic Subunit Ssp2 and Glycogen Synthase Kinases Gsk3 and Gsk31 in Schizosaccharomyces Pombe.

    Science.gov (United States)

    Qingyun; Ma, Yan; Kato, Toshiaki; Furuyashiki, Tomoyuki

    2016-01-01

    In Schizosaccharomyces pombe, Ssp2, an ortholog of AMP-activated protein kinase (AMPK), is critical for cell growth at restrictive temperatures and under glucose depletion as well as sexual differentiation under nitrogen depletion. To identify genes genetically related to Ssp2, we performed a genetic screening to search for the genes whose overexpression rescued the growth defects in Δssp2 cells at restrictive temperatures, and identified 35 cosmids as multicopy suppressor genes. In Southern blot analyses, 22 out of these cosmids were hybridized to an ssp2+ probe. Using nucleotide sequencing, we identified the gsk3+ gene in one of the cosmids, and the remaining 12 cosmids were hybridized to a gsk3+ probe. Overexpression of the gsk3+ gene or the gsk31+ gene, another GSK3 member, rescues defective growth of Δssp2 cells at restrictive temperatures and under glucose depletion as well as sexual differentiation under nitrogen depletion. Δgsk3Δgsk31 double knockout cells, but neither Δgsk3 nor Δgsk31 single knockout cells, phenocopy Δssp2 cells. The deletion of the gsk3+ or gsk31+ gene augments the phenotypes of Δssp2 cells. These findings suggest that Gsk3 and Gsk31 are critical and interact with Ssp2 in multiple cellular functions. PMID:27604537

  12. Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe.

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    Alba Gómez-Hierro

    Full Text Available Calcium/calmodulin-dependent protein kinase kinase (CaMKK is required for diverse cellular functions. Mammalian CaMKK activates CaMKs and also the evolutionarily-conserved AMP-activated protein kinase (AMPK. The fission yeast Schizosaccharomyces pombe CaMKK, Ssp1, is required for tolerance to limited glucose through the AMPK, Ssp2, and for the integration of cell growth and division through the SAD kinase Cdr2.Here we report that Ssp1 controls the G2/M transition by regulating the activity of the CaMK Srk1. We show that inhibition of Cdc25 by Srk1 is regulated by Ssp1; and also that restoring growth polarity and actin localization of ssp1-deleted cells by removing the actin-monomer-binding protein, twinfilin, is sufficient to suppress the ssp1 phenotype.These findings demonstrate that entry into mitosis is mediated by a network of proteins, including the Ssp1 and Srk1 kinases. Ssp1 connects the network of components that ensures proper polarity and cell size with the network of proteins that regulates Cdk1-cyclin B activity, in which Srk1 plays an inhibitory role.

  13. Lactobacillus rhamnosus HN001 attenuates allergy development in a pig model.

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    Debra J Thomas

    Full Text Available BACKGROUND: Probiotics have been studied as immunomodulatory agents of allergy. Several human probiotic trials tracking the development of eczema and other forms of allergy have yielded inconsistent results. A recent infant study demonstrated that pre and postnatal Lactobacillus rhamnosus HN001 (HN001 supplementation decreased the prevalence of eczema and IgE associated eczema. However, the influence of HN001 on the incidence of wheeze, asthma, and/or other allergic manifestations has yet to be reported. OBJECTIVE: This study was conducted to determine the effects of the probiotic HN001 on the development of allergic lung disease in a pig model. METHODS: Allergy was induced by a series of subcutaneous and intratracheal sensitizations with Ascaris suum allergen (ASA during a six week time frame in post-weanling pigs supplemented daily with HN001, or without supplementation. One week following final sensitization intradermal skin tests and respiratory challenges were conducted. RESULTS: In response to intradermal and respiratory challenges, ASA-sensitized pigs fed HN001 had less severe skin flare reactions, smaller increases in pleural pressure, and trends towards lower changes in arterial oxygen and carbon dioxide partial pressure levels compared to control pigs. The frequency of ASA-specific IFN-γ-secreting peripheral blood mononuclear cells, as well as the amount of IL-10 produced by ASA-specific cells, was of greater magnitude in probiotic-fed pigs compared to control animals. These observations suggest that differences in clinical responses to the allergen challenges may be related to probiotic-induced modulation of Th1 (IFN-γ and regulatory (IL-10 cytokine expression. CONCLUSIONS: Probiotic supplementation decreased the severity of allergic skin and lung responses in allergen-sensitized pigs with a corresponding increase in IFN-γ expression. A similar correlation between certain allergic responses and increased IFN-γ expression has been

  14. Effects of the viability of Lactobacillus rhamnosus GG on rotavirus infection in neonatal rats

    Institute of Scientific and Technical Information of China (English)

    Hanna Ventola; Liisa Lehtoranta; Mari Madetoja; Marja-Leena Simonen-Tikka; Leena Maunula; Merja Roivainen; Riitta Korpela; Reetta Holma

    2012-01-01

    AIM:To study the effects of live and dead Lactobacillus rhamnosus GG (GG) on rotavirus infection in a neonatal rat model.METHODS:At the age of 2 d,suckling Lewis rat pups were supplemented with either live or dead GG and the treatment was continued daily throughout the experiment.At the age of 5 and 6 d the pups received oral rotavirus (RV) SA-11 strain.The pups were sacrificed at the age of 7 or 8 d by decapitation.The gastrointestinal tract was removed and macroscopic observations were done.The consistency of feces in the colon was classified using a four-tier system.RV was detected from the plasma,small intestine,colon and feces by real-time quantitative polymerase chain reaction (PCR).RESULTS:In this neonatal rat model,RV induced a mild-to-moderate diarrhea in all except one pup of the RV-inoculated rats.RV moderately reduced body weight development from day 6 onwards.On day 7,after 2 d of RV infection,live and dead GG groups gained significantly more weight than the RV group without probiotics [36% (P =0.001) and 28% (P =0.031),respectively].In addition,when compared with the RV control group,both live and dead GG reduced the weight ratio of colon/animal body weight to the same level as in the healthy control group,with reductions of 22% (P=0.002) and 28% (P < 0.001),respectively.Diarrhea increased moderately in both GG groups.However,the diarrhea incidence and severity in the GG groups were not statistically significantly different as compared with the RV control group.Moreover,observed diarrhea did not provoke weight loss or death.The RV control group had the largest amount of RV PCR-positive samples among the RV-infected groups,and the live GG group had the smallest amount.Rats receiving live GG had significantly less RV in the colon (P =0.027) when compared with the RV control group.Live GG was also more effective over dead GG in reducing the quantity of RV from plasma (P =0.047).CONCLUSION:Both live and dead GG have beneficial effects in RV

  15. Synergistic Inhibition of Lactobacillus Rhamnosus and Cisplatin on the Multiplication of Tumoral Cells in BALB/c Mice with Breast Cancer

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    Ghaderi Pakdel

    2011-12-01

    Full Text Available Introduction: The probiotic strains of Lactic Acid Bacillus (LAB not only affect gastrointestinal tract microflora and stimulate local immune system of this tract but also modify and stimulate systemic immunity by influence on lymph nodes and spleen. Several studies have shown the anti-tumor effect of these kinds of bacteria. This study was designed to assess the probiotic effects of lactobacillus rhamnosus on cisplatin efficacy among Balb/c mice with breast cancer. Methods: L. rhamnosus strain was inoculated in MRS agar and cultivated for 24 h at 37 °C. Female BalbC mice (n=20 with invasive ductal carcinoma transplantation were divided into four groups: Control, L. rhamnosus, cisplatin and cisplatin plus L. rhamnosus. Cisplatin (5 mg/kg, i.p. was injected twice a week. Lr was administered daily by gastric intubation (3×10 8 CFU/day. The tumor size was measured every 3 days and mice were sacrificed 24 h after the last injection and tumor tissue was removed for more tests. Results: The results showed that oral administration of L. rhamnosus decreased the growth rate of tumor (p<0.05. One reason for antineoplastic effect of lactobacilli is immune system enhancement. The results of delayed-type hypersensitivity show the stimulation of immune system and inhibition of tumor growth by this mechanism. In pathologic assessments probiotic administration increased the antineoplastic effect of cisplatin. Conclusion: According to the findings of this study it can be expected that human studies also show the satisfactory effect of lactobacillus administration besides common therapeutic methods for cancer treatment.

  16. A Novel Bluetooth Man-In-The-Middle Attack Based On SSP using OOB Association model

    CERN Document Server

    Saravanan, K; Negesh, R K

    2012-01-01

    As an interconnection technology, Bluetooth has to address all traditional security problems, well known from the distributed networks. Moreover, as Bluetooth networks are formed by the radio links, there are also additional security aspects whose impact is yet not well understood. In this paper, we propose a novel Man-In-The-Middle (MITM) attack against Bluetooth enabled mobile phone that support Simple Secure Pairing(SSP). From the literature it was proved that the SSP association models such as Numeric comparison, Just works and passkey Entry are not more secure. Here we propose the Out Of Band (OOB) channeling with enhanced security than the previous methods.

  17. INDUSTRIJSKA KONOPLJA (Cannabis sativa L. ssp. sativa) V SLOVENIJI IN PO SVETU

    OpenAIRE

    Medved, Meta

    2012-01-01

    Gojenje industrijske konoplje (Cannabis sativa L. ssp. sativa) ima v Sloveniji in po svetu še vedno negativen prizvok. Razlog za to je sorodnost te rastline z indijsko konopljo (Cannabis sativa L. ssp. indica), ki se vzgaja za pridobivanje droge. To se odraža tudi v zakonodaji, ki nekaterim državam prepoveduje, drugim pa omejuje gojenje konoplje. Namen te raziskave je predstaviti uporabnost in dobičkonosnost pri pridelavi konoplje, ter preveriti vpliv pojavnosti droge, indijske konoplje na ...

  18. The catalase gene differentiates between some strains of Staphylococcus aureus ssp. anaerobius

    OpenAIRE

    Musa, N. O.; Eltom, K.; Gessler, F.; H. Böhnel; Babiker, A.; Sanousi, S. M.

    2010-01-01

    Staphylococcus aureus ssp anaerobius strain S10 was isolated from an outbreak of sheep abscess disease. Sequence of the catalase gene of this strain showed 99 % identity to the catalase gene (katB) sequence of the reference strain (S. aureus ssp. anaerobius strain MVF213) with mismatching of three base pairs. An important substitution located 1036 nucleotides upstream of the initiation codon from “C” in katB to “T” in the catalase gene of strain S10 originated a stop codon. The de...

  19. Analysis of COPII Vesicles Indicates a Role for the Emp47-Ssp120 Complex in Transport of Cell Surface Glycoproteins.

    Science.gov (United States)

    Margulis, Neil G; Wilson, Joshua D; Bentivoglio, Christine M; Dhungel, Nripesh; Gitler, Aaron D; Barlowe, Charles

    2016-03-01

    Coat protein complex II (COPII) vesicle formation at the endoplasmic reticulum (ER) transports nascent secretory proteins forward to the Golgi complex. To further define the machinery that packages secretory cargo and targets vesicles to Golgi membranes, we performed a comprehensive proteomic analysis of purified COPII vesicles. In addition to previously known proteins, we identified new vesicle proteins including Coy1, Sly41 and Ssp120, which were efficiently packaged into COPII vesicles for trafficking between the ER and Golgi compartments. Further characterization of the putative calcium-binding Ssp120 protein revealed a tight association with Emp47 and in emp47Δ cells Ssp120 was mislocalized and secreted. Genetic analyses demonstrated that EMP47 and SSP120 display identical synthetic positive interactions with IRE1 and synthetic negative interactions with genes involved in cell wall assembly. Our findings support a model in which the Emp47-Ssp120 complex functions in transport of plasma membrane glycoproteins through the early secretory pathway.

  20. FORMULASI PRODUK SUSU FERMENTASI KERING DENGAN PENAMBAHAN BAKTERI PROBIOTIK Lactobacillus casei DAN Bifidobacterium longum

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    Dida Hani Rahman

    2012-11-01

    Full Text Available ABSTRACTFermented milk is a healthy product that has many benefits especially for human digestive tract. Manufacturing of probiotic fermented milk products as a functional food with a viable long shelf life needs to be developed. The purpose of this study was to formulate a dried fermented milk product using probiotic bacterias. The experimental design study was a complete random design with 4 treatments using different lactic acid bacteria (LAB: A1 (Streptococcus lactis: 0.5%; A2 (Streptococcus lactis: 0.25% and Lactobacillus casei: 0.25%; A3 (Streptococcus lactis: 0.25%, Lactobacillus bulgaricus: 0.125%, Streptococcus thermophiles: 0.125%; and A4 (Streptococcus lactis: 0.25% and Bifidobacterium longum: 0.25%. The highest level of hardness was A2 product and the highest level of tenderness is A1 product. Results of proximate analysis showed that dried fermented milk products had high levels of the protein, calcium, and phosphorus. Microbiological test results showed that the amount of lactic acid bacteria (BAL in dried fermented milk products were eligible based on CODEX: 243 (2003. Statictical analysis using ANOVA in the hedonic quality test showed that the treatments were significantly different (p 0.05 on the attributes of color, aroma, hardness, and flavor except texture.Key words: dried fermented milk, functional food, probiotic, lactic acid bacteria (LABABSTRAKSusu fermentasi merupakan produk kesehatan yang mempunyai banyak manfaat terutama untuk saluran pencernaan manusia. Pembuatan produk susu fermentasi probiotik sebagai makanan fungsional yang mempunyai kelangsungan hidup dan daya simpan yang lama perlu dikembangan. Tujuan penelitian ini adalah untuk memformulasikan susu fermentasi kering menggunakan bakteri probiotik. Desain penelitian yang digunakan adalah rancangan acak lengkap dengan 4 perlakuan menggunakan 4 bakteri asam laktat (BAL, (Streptococcus lactis, 0.5%, A2 (Streptococcus lactis; 0.25% dan Lactobacillus casei; 0.25%, A3

  1. Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG.

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    Ingemar von Ossowski

    Full Text Available Primarily arising from their well understood beneficial health effects, many lactobacilli strains are considered good candidates for use as probiotics in humans and animals. Lactobacillar probiosis can itself be best typified by the Lactobacillus rhamnosus GG strain, which, with its well-documented clinical benefits, has emerged as one of the most widely used probiotics in the food and health-supplement industries. Even so, many facets of its molecular mechanisms and limitations as a beneficial commensal bacterium still remain to be thoroughly explored and dissected. Because L. rhamnosus GG is one of only a few such strains exhibiting surface piliation (called SpaCBA, we sought to examine whether this particular type of cell-surface appendage has a discernible immunomodulating capacity and is able to trigger targeted responses in human immune-related cells. Thus, presented herein for this study, we recombinantly engineered Lactococcus lactis to produce native (and pilin-deleted SpaCBA pili that were assembled in a structurally authentic form and anchored to the cell surface, and which had retained mucus-binding functionality. By using these recombinant lactococcal constructs, we were able to demonstrate that the SpaCBA pilus can be a contributory factor in the activation of Toll-like receptor 2-dependent signaling in HEK cells as well as in the modulation of pro- and anti-inflammatory cytokine (TNF-α, IL-6, IL-10, and IL-12 production in human monocyte-derived dendritic cells. From these data, we suggest that the recombinant-expressed and surface-anchored SpaCBA pilus, given its projected functioning in the gut environment, might be viewed as a new microbe-associated molecular pattern (MAMP-like modulator of innate immunity. Accordingly, our study has brought some new insight to the molecular immunogenicity of the SpaCBA pilus, thus opening the way to a better understanding of its possible role in the multifaceted nature of L. rhamnosus GG

  2. Lactobacillus casei triggers a TLR mediated RACK-1 dependent p38 MAPK pathway in Caenorhabditis elegans to resist Klebsiella pneumoniae infection.

    Science.gov (United States)

    Kamaladevi, Arumugam; Balamurugan, Krishnaswamy

    2016-07-13

    In the present study, the effect of Lactic Acid Bacteria (LAB) was investigated at the molecular level using the model organism Caenorhabditis elegans against Klebsiella pneumoniae. Out of the 13 LAB screened, Lactobacillus casei displayed excellent protective efficacy by prolonging the survival of K. pneumoniae-infected nematodes. Pretreatment with L. casei significantly decreased bacterial colonization and rescued K. pneumoniae-infected C. elegans from various physiological impairments. The concomitant upregulation of key immune genes that regulate the TLR, RACK-1 as well as the p38 MAPK pathway rather than the IIS and ERK pathway suggested that the plausible immunomodulatory mechanism of L. casei could be by triggering the TLR, RACK-1 and p38 MAPK pathway. Furthermore, the hyper-susceptibility of L. casei treated loss-of-function mutants of the tol-1, RACK-1 and p38 MAPK pathway (sek-1 and pmk-1) to K. pneumoniae infection and gene expression analysis suggested that L. casei triggered a TLR mediated RACK-1 dependent p38 MAPK pathway to increase host resistance and protect nematodes against K. pneumoniae infection. PMID:27338631

  3. Anti-inflammatory effects of Lactobacillus casei BL23 producing or not a manganese-dependant catalase on DSS-induced colitis in mice

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    Corthier Gérard

    2007-07-01

    Full Text Available Abstract Background Human immune cells generate large amounts of reactive oxygen species (ROS throughout the respiratory burst that occurs during inflammation. In inflammatory bowel diseases, a sustained and abnormal activation of the immune system results in oxidative stress in the digestive tract and in a loss of intestinal homeostasis. We previously showed that the heterologous production of the Lactobacillus plantarum ATCC14431 manganese-dependant catalase (MnKat in Lb. casei BL23 successfully enhances its survival when exposed to oxidative stress. In this study, we evaluated the preventive effects of this antioxidative Lb. casei strain in a murine model of dextran sodium sulfate (DSS-induced moderate colitis. Results Either Lb. casei BL23 MnKat- or MnKat+ was administered daily to mice treated with DSS for 10 days. In contrast to control mice treated with PBS for which DSS induced bleeding diarrhea and mucosal lesions, mice treated with both Lb. casei strains presented a significant (p Conclusion No contribution of MnKat to the protective effect from epithelial damage has been observed in the tested conditions. In contrast, these results confirm the high interest of Lb. casei as an anti-inflammatory probiotic strain.

  4. Simultaneous Production of Biosurfactants and Bacteriocins by Probiotic Lactobacillus casei MRTL3

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    Deepansh Sharma

    2014-01-01

    Full Text Available Lactic acid bacteria (LAB are ubiquitous and well-known commensal bacteria in the human and animal microflora. LAB are extensively studied and used in a variety of industrial and food fermentations. They are widely used for humans and animals as adjuvants, probiotic formulation, and dietary supplements and in other food fermentation applications. In the present investigation, LAB were isolated from raw milk samples collected from local dairy farms of Haryana, India. Further, the isolates were screened for simultaneous production of biosurfactants and bacteriocins. Biosurfactant produced was found to be a mixture of lipid and sugar similar to glycolipids. The bacteriocin obtained was found to be heat stable (5 min at 100°C. Further, DNA of the strain was extracted and amplified by the 16S rRNA sequencing using universal primers. The isolate Lactobacillus casei MRTL3 was found to be a potent biosurfactant and bacteriocin producer. It seems to have huge potential for food industry as a biopreservative and/or food ingredient.

  5. Development of Corn Milk Yoghurt Using Mixed Culture of Lactobacillus delbruekii, Streptococcus salivarus, and Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Sedarnawati Yasni

    2014-03-01

    Full Text Available The main objective of this research was to diversify the utilization of corn as commercial commodity by a corn-based new product development in the form of yoghurt. The first step was carried out to make corn yoghurt with the addition of fresh milk into heated corn extract using mixture starter of Lactobacillus delbruekii, Streptococcus salivarus, and Lactobacillus casei and the best formulation was determined through hedonic evaluation along with weighing method. The selected formulation from this step was corn extract with the addition of 50% fresh milk. The corn extract was produced from corn kernels that were blended and mixed with water in ratio of 3:1, the mixture was further heated and concentrated until the total volume remained 2/3. Afterwards, this selected formulation was added with sugar and full cream milk powder. The hedonic evaluation results showed that the mixture with 10% sugar and 5% full cream milk powder addition possessed the highest score. Lastly, the selected formulation was observed for physical, microbiological, and chemical assay during 4 weeks period. The ultimate observation concluded that the product could be classified as probiotics with total lactic acid bacteria reached 1.5 x 109 CFU/ml with medium fat content (1.8%.

  6. L-lactic acid production from Lactobacillus casei by solid state fermentation using rice straw

    Directory of Open Access Journals (Sweden)

    Risheng, Yao

    2007-01-01

    Full Text Available In order to make full use of rice straw (RS produced at large quantity in China and to reduce the production cost of L-lactic acid, attempts were made to utilize the hydrolysate of RS as sole carbon source and the lignocellulose as inert support for producing L-lactic acid using solid state fermentation (SSF. The pretreated rice straw was enzymatically hydro- lyzed by cellulase, and the hydrolyzate, containing reducing sugars supplemented with a minimum of (NH42SO4, MnSO4, and yeast extract, was used as moistening agent to impregnate 5g of RS, which was used as the inert support for SSF. Maximum L-lactic acid production of 3.467g per 5g of support was obtained at 37 oC, using Lactobacillus casei as inoculum, after 5 days of fermentation with optimized process parameters such as 72% moisture content, 4g per 5g support of reducing sugars, 2.5ml per 5g support of inoculum size, 3g per 5g support of CaCO3, and pH 6.5.

  7. In Vitro Investigation of the Immunomodulatory Potential of Probiotic Lactobacillus casei.

    Science.gov (United States)

    Karthikeyan, Thirugnanam; Pravin, Mariappan; Muthusamy, Velusamy Shanmuganathan; Bharathi Raja, Rajaganapathy; Lakshmi, Baddireddi Subhadra

    2013-03-01

    The current study investigated the immunomodulatory potential of ethyl acetate soluble supernatant of Lactobacillus casei (LC-EAS) in vitro. The effect of LC-EAS on nitric oxide release was analyzed in RAW 264.7 cells, wherein, an inhibition in nitric oxide production through suppression of inducible nitric oxide synthase mRNA expression was observed. Evaluation of LC-EAS on LPS-induced peripheral blood mononuclear cells showed a down-regulation in TNF-α and IL-6 genes and an upregulation of IL-10. An inhibition in the protein expression of NF-κB, ERK1/2 and STAT3 phosphorylation confirms the immunomodulatory potential of LC-EAS. The effect of LC-EAS on in vitro intestinal epithelial cells was investigated using HT-29 human colon adenocarcinoma cancer cells. LC-EAS exhibited an inhibition of NF-κB and ERK1/2 phosphorylation, whereas STAT3 phosphorylation was unregulated. To evaluate the downstream target of STAT3 upregulation, expression of the intestinal trefoil factor TFF3 which is a NF-κB regulator and STAT3 downstream target was studied. LC-EAS was observed to elevate TFF3 mRNA expression. Overall the study shows that the anti-inflammatory potential of LC-EAS is through inhibition of NF-κB in different cell types. PMID:26782605

  8. In Vitro Investigation of the Immunomodulatory Potential of Probiotic Lactobacillus casei.

    Science.gov (United States)

    Karthikeyan, Thirugnanam; Pravin, Mariappan; Muthusamy, Velusamy Shanmuganathan; Bharathi Raja, Rajaganapathy; Lakshmi, Baddireddi Subhadra

    2013-03-01

    The current study investigated the immunomodulatory potential of ethyl acetate soluble supernatant of Lactobacillus casei (LC-EAS) in vitro. The effect of LC-EAS on nitric oxide release was analyzed in RAW 264.7 cells, wherein, an inhibition in nitric oxide production through suppression of inducible nitric oxide synthase mRNA expression was observed. Evaluation of LC-EAS on LPS-induced peripheral blood mononuclear cells showed a down-regulation in TNF-α and IL-6 genes and an upregulation of IL-10. An inhibition in the protein expression of NF-κB, ERK1/2 and STAT3 phosphorylation confirms the immunomodulatory potential of LC-EAS. The effect of LC-EAS on in vitro intestinal epithelial cells was investigated using HT-29 human colon adenocarcinoma cancer cells. LC-EAS exhibited an inhibition of NF-κB and ERK1/2 phosphorylation, whereas STAT3 phosphorylation was unregulated. To evaluate the downstream target of STAT3 upregulation, expression of the intestinal trefoil factor TFF3 which is a NF-κB regulator and STAT3 downstream target was studied. LC-EAS was observed to elevate TFF3 mRNA expression. Overall the study shows that the anti-inflammatory potential of LC-EAS is through inhibition of NF-κB in different cell types.

  9. Optimization of probiotic lactobacillus casei ATCC 334 production using date powder as carbon source

    Directory of Open Access Journals (Sweden)

    Shahravy A.

    2012-01-01

    Full Text Available This study was conducted to optimize culture conditions for economic production of a probiotic bacterium, Lactobacillus casei ATCC 334, in which palm date powder was applied for the first time as a low-cost main carbon source. The effect of eleven factors on bacterial growth was investigated using the Taguchi experimental design, and three factors including palm date powder, tryptone and agitation rate were found to be the most significant parameters. The optimum conditions including date powder concentration, 38 g/L; tryptone concentration, 30 g/L; and an agitation rate of 320 rpm were determined by response surface methodology of Box-Behnken. A third-order polynomial model was suggested to predict the design space following which the predicted values were validated experimentally. The maximum log value of the viable cells in the optimized alternative medium was 9.97 at 24 h of incubation which was comparable to that obtained in the complex and expensive MRS medium (10.06.

  10. Valorisation of food waste via fungal hydrolysis and lactic acid fermentation with Lactobacillus casei Shirota.

    Science.gov (United States)

    Kwan, Tsz Him; Hu, Yunzi; Lin, Carol Sze Ki

    2016-10-01

    Food waste recycling via fungal hydrolysis and lactic acid (LA) fermentation has been investigated. Hydrolysates derived from mixed food waste and bakery waste were rich in glucose (80.0-100.2gL(-1)), fructose (7.6gL(-1)) and free amino nitrogen (947-1081mgL(-1)). In the fermentation with Lactobacillus casei Shirota, 94.0gL(-1) and 82.6gL(-1) of LA were produced with productivity of 2.61gL(-1)h(-1) and 2.50gL(-1)h(-1) for mixed food waste and bakery waste hydrolysate, respectively. The yield was 0.94gg(-1) for both hydrolysates. Similar results were obtained using food waste powder hydrolysate, in which 90.1gL(-1) of LA was produced with a yield and productivity of 0.92gg(-1) and 2.50gL(-1)h(-1). The results demonstrate the feasibility of an efficient bioconversion of food waste to LA and a decentralized approach of food waste recycling in urban area. PMID:26873283

  11. Efecto de Lactobacillus casei sobre la incidencia de procesos infecciosos en niños/as Effect of lactobacillus casei on the incidence of infectious conditions in children

    Directory of Open Access Journals (Sweden)

    J. M.ª Cobo Sanz

    2006-08-01

    Full Text Available Objetivo: Analizar el efecto del consumo continuado de leche fermentada con Lactobacillus casei (DN-114001 (Actimel" sobre la incidencia de los trastornos infecciosos comunes en niños. Ámbito: Población escolar infantil. Sujetos: alumnos de 3 a 12 años de dos centros de educación infantil y primaria de Barcelona. En el estudio participaron un total de 251 niños de ambos sexos. Intervenciones: Se realizó un estudio de intervención nutricional durante 20 semanas con un diseño paralelo, prospectivo, doble ciego y aleatorizado por clusters pragmáticos, en el que participaron los alumnos de dos centros de educación infantil y primaria. Uno de los centros fue asignado a recibir dos unidades diarias de Actimel", y el otro a dos unidades de Placebo. De los 251 niños de ambos sexos que participaron en el estudio, 109 recibieron placebo y 142 recibieron Actimel". Se recogieron las características demográficas y de antecedentes clínicos basales, y se realizaron controles de los síntomas relacionados con los trastornos infecciosos u otras enfermedades a las 12, 16 y 20 semanas. El análisis de los datos se realizó en la población por intención de tratar (ITT, siendo la variable principal la duración de los síntomas respiratorios y gastrointestinales. Resultados: Se encontró una diferencia, aunque no fue significativa, de un día en la mediana de duración total de días con enfermedad a lo largo del estudio (grupo Actimel®: 1 día vs grupo placebo: 2 días. También se observó esta misma diferencia no significativa en la duración de días con enfermedades respiratorias (vías respiratorias altas y bajas y enfermedades gastrointestinales (diarrea, vómitos, dolor de estómago y estreñimiento. Sí se encontró una diferencia estadísticamente significativa en favor del grupo Actimel® en la duración de las enfermedades de vías respiratorias bajas, bronquitis o neumonía y en la duración de la fatiga. Asimismo, se detectó una

  12. Full Scale Test of SSP 34m blade, edgewise loading LTT

    DEFF Research Database (Denmark)

    Nielsen, Magda; Jensen, Find Mølholt; Nielsen, Per Hørlyk;

    This report is a part of the research project “Eksperimentel vingeforskning: Strukturelle mekanismer i nutidens og fremtidens store vinger under kombineret last” where a 34m wind turbine blade from SSP-Technology A/S has been tested in edgewise direction (LTT). The applied load is 60% of an unrea...

  13. Full Scale Test SSP 34m blade, Combined load. Data report

    DEFF Research Database (Denmark)

    Nielsen, Per Hørlyk; Nielsen, Magda; Jensen, Find Mølholt;

    This report is part of the research project entitled “Eksperimentel vingeforskning: Strukturelle mekanismer i nutidens og fremtidens store vinger under kombineret last” where a 34m wind turbine blade from SSP-Technology A/S was tested in combined flap and edgewise load. The applied load is 55% of...

  14. Proteomic analysis of bronchoalveolar lavage fluid proteins from mice infected with Francisella tularensis ssp novicida

    Energy Technology Data Exchange (ETDEWEB)

    Varnum, Susan M.; Webb-Robertson, Bobbie-Jo M.; Pounds, Joel G.; Moore, Ronald J.; Smith, Richard D.; Frevert, Charles; Skerret, Shawn J.; Wunschel, David S.

    2012-07-06

    Francisella tularensis causes the zoonosis tularemia in humans and is one of the most virulent bacterial pathogens. We utilized a global proteomic approach to characterize protein changes in bronchoalveolar lavage fluid from mice exposed to one of three organisms, F. tularensis ssp. novicida, an avirulent mutant of F. tularensis ssp. novicida (F.t. novicida-ΔmglA); and Pseudomonas aeruginosa. The composition of BALF proteins was altered following infection, including proteins involved in neutrophil activation, oxidative stress and inflammatory responses. Components of the innate immune response were induced including the acute phase response and the complement system, however the timing of their induction varied. Francisella tularensis ssp. novicida infected mice do not appear to have an effective innate immune response in the first hours of infection, however within 24 hours they show an upregulation of innate immune response proteins. This delayed response is in contrast to P. aeruginosa infected animals which show an early innate immune response. Likewise, F.t. novicida-ΔmglA infection initiates an early innate immune response, however this response is dimished by 24 hours. Finally, this study identifies several candidate biomarkers, including Chitinase 3-like-1 (CHI3L1 or YKL-40) and peroxiredoxin 1, that are associated with F. tularensis ssp. novicida but not P. aeruginosa infection.

  15. PENGEMBANGAN SSP FISIKA BERBASIS PENDEKATAN CTL UNTUK MENINGKATKAN KETERAMPILAN PROSES SAINS DAN MOTIVASI BELAJAR

    Directory of Open Access Journals (Sweden)

    Faiq Makhdum Noor

    2015-04-01

    Full Text Available This research aims to develop a learning device as Subject-Specific Pedagogy (SSP of physics, and to improve science process skills and learning motivation of the students. This research used the 4D model. The subjects of this research were the XI grade students of Muhammadiyah High School 2 Yogyakarta. The data collecting instruments were expert validation sheets, science process skill observation sheets, questionnaire of students’ response to the student books and worksheets, question-naire of students’ response to teaching process, students’ motivation learning sheets, and science process skill paper test. The research data were collected through observation, questionnaire, and paper test, and analyzed quantitatively. The results show as follows. (1 The development of physics SSP is carried out in accordance with the procedures of 4D model product development. (2 The results of expert’s validation show the physics SSP is in the very good criteria. (3 The readability of the student books and worksheets is in the good criteria. (4 The increasing of the score of science process skills and students’ learning motivation in the experimental class is higher than that in the control class. This shows that the physics SSP is fit for use in physics teaching.

  16. The discovery of resistant sources of spring barley, Hordeum vulgare ssp. spontaneum, and unique greenbug biotypes

    Science.gov (United States)

    The genetic sources for host-plant resistance to the greenbug (Schiazphis graminum Ronani) in barley (Hordeum vulgare ssp. spontaneum) are limited in that only two single dominant genes Rsg1 and Rsg2 are available for resistance to greenbug biotypes. We evaluated four new barley lines from the Wild...

  17. Adhesion of Human Probiotic Lactobacillus rhamnosus to Cervical and Vaginal Cells and Interaction with Vaginosis-Associated Pathogens

    Directory of Open Access Journals (Sweden)

    Sophie Coudeyras

    2008-01-01

    Full Text Available Objectives. The ability of a probiotic Lactobacillus rhamnosus strain (Lcr35 to adhere to cervical and vaginal cells and to affect the viability of two main vaginosis-associated pathogens, Prevotella bivia, Gardnerella vaginalis, as well as Candida albicans was investigated. Methods. Adhesion ability was determined in vitro with immortalized epithelial cells from the endocervix, ectocervix, and vagina. Coculture experiments were performed to count viable pathogens cells in the presence of Lcr35. Results. Lcr35 was able to specifically and rapidly adhere to the three cell lines. In coculture assays, a decrease in pathogen cell division rate was observed as from 4 hours of incubation and bactericidal activity after a longer period of incubation, mostly with P. bivia. Conclusion. The ability of Lcr35 to adhere to cervicovaginal cells and its antagonist activities against vaginosis-associated pathogens suggest that this probiotic strain is a promising candidate for use in therapy.

  18. Crude goat whey fermentation by Kluyveromyces marxianus and Lactobacillus rhamnosus: contribution to proteolysis and ACE inhibitory activity.

    Science.gov (United States)

    Hamme, Vanessa; Sannier, Frederic; Piot, Jean-Marie; Didelot, Sandrine; Bordenave-Juchereau, Stephanie

    2009-05-01

    Unsupplemented acid goat whey containing 0.96% protein and 2.76% lactose was fermented aerobically with 32 microflora extracted from various raw milk cheeses and dairy products. These microflora were screened for their ability to hydrolyse whey proteins (alpha-lactalbumin and/or beta-lactoglobulin) and to generate peptides inhibitors of Angiotensin I Converting Enzyme. Five microflora were able to degrade whey protein. The most efficient microflora was able to fully hydrolyse alpha-lactalbumin and to a lesser extend beta-lactoglobulin. It was extracted from Bamalou des Pyrenées cheese. Micro-organisms involved consisted of yeast Kluyveromyces marxianus and lactobacillus Lactobacillus rhamnosus. Both were able to produce ACE inhibitory peptides after whey fermentation. PMID:19121243

  19. Risks associated with high-dose Lactobacillus rhamnosus in an Escherichia coli model of piglet diarrhoea: intestinal microbiota and immune imbalances.

    Directory of Open Access Journals (Sweden)

    Xiao-Qiong Li

    Full Text Available Probiotic could be a promising alternative to antibiotics for the prevention of enteric infections; however, further information on the dose effects is required. In this study, weanling piglets were orally administered low- or high-dose Lactobacillus rhamnosus ACTT 7469 (10(10 CFU/d or 10(12 CFU/d for 1 week before F4 (K88-positive Escherichia coli challenge. The compositions of faecal and gastrointestinal microbiota were recorded; gene expression in the intestines was assessed by real-time PCR; serum tumour necrosis factor-α (TNF-α concentrations and intestinal Toll-like receptor 4 (TLR4 were detected by ELISA and immunohistochemistry, respectively. Unexpectedly, high-dose administration increased the incidence of diarrhoea before F4(+ETEC challenge, despite the fact that both doses ameliorated F4(+ETEC-induced diarrhoea with increased Lactobacillus and Bifidobacterium counts accompanied by reduced coliform shedding in faeces. Interestingly, L. rhamnosus administration reduced Lactobacillus and Bifidobacterium counts in the colonic contents, and the high-dose piglets also had lower Lactobacillius and Bacteroides counts in the ileal contents. An increase in the concentration of serum TNF-α induced by F4(+ETEC was observed, but the increase was delayed by L. rhamnosus. In piglets exposed to F4(+ETEC, jejunal TLR4 expression increased at the mRNA and protein levels, while jejunal interleukin (IL-8 and ileal porcine β-defensins 2 (pBD2 mRNA expression increased; however, these increases were attenuated by administration of L. rhamnosus. Notably, expression of jejunal TLR2, ileal TLR9, Nod-like receptor NOD1 and TNF-α mRNA was upregulated in the low-dose piglets after F4(+ETEC challenge, but not in the high-dose piglets. These findings indicate that pretreatment with a low dose of L. rhamnosus might be more effective than a high dose at ameliorating diarrhoea. There is a risk that high-dose L. rhamnosus pretreatment may negate the preventative

  20. Effect of Lactobacillus paracasei subsp. paracasei, L. casei 431 on immune response to influenza vaccination and upper respiratory tract infections in healthy adult volunteers

    DEFF Research Database (Denmark)

    Jespersen, Lillian; Tarnow, Inge; Eskesen, Dorte;

    2015-01-01

    . Hansen A/S) (hereafter, L. casei 431) on immune response to influenza vaccination and respiratory symptoms in healthy adults. DESIGN: A randomized double-blind, placebo-controlled trial was conducted in 1104 healthy subjects aged 18-60 y at 2 centers in Germany and Denmark. Subjects were randomly...... assigned to receive an acidified milk drink containing ≥10(9) colony-forming units of L. casei 431 (n = 553) or placebo (n = 551) for 42 d. After 21 d, subjects received the seasonal influenza vaccination. Primary outcome was seroprotection rate (anti-influenza antibody titers by hemagglutination...... inhibition) 21 d after vaccination. Other outcomes were seroconversion rate and mean titers, influenza A-specific antibodies and incidence, and duration and severity of upper respiratory symptoms. Antibiotic use and use of health care resources were recorded. RESULTS: There was no effect of L. casei 431...

  1. 益生菌Lactobacillus casei Zhang高密度发酵中试工艺及动力学研究%Pilot Plant Scale Test and Kinetic Studies on High Cell Density Culture of Probiotics Lactobacillus casei Zhang

    Institute of Scientific and Technical Information of China (English)

    李妍; 高鹏飞; 赵文静; 麻士卫; 周琦; 潘向辉; 张和平

    2008-01-01

    在益生菌L. Casei Zhang高密度培养小试(3L)基础上,进行30L到150L逐级放大中试生产工艺的研究以确定规模化生产工艺.在优化的发酵工艺下,150L规模发酵菌体密度可达2.9×1010cfu/mL,与小试水平无差异.采用origin7.5软件在logistic equation基础上建立L casei Zhang的生长和葡萄糖代谢动力学模型,模型与试验值拟合良好,平均误差小于10%,能够较好地反应发酵过程.初步探讨发酵后菌体的离心和冷冻干燥过程对菌体的影响,虽然发酵液经离心收集菌体后冷冻干燥可得到平均活菌数2.65×1011cfu/g的菌粉,能够满足益生菌制剂和发酵剂对高活菌数的要求,但冻干前后活菌得率仅49.97%.有必要针对L caseiZhang的冻干保护剂和冻干工艺进一步优化,以提高菌体存活率得到更高菌体浓度的益生菌粉.

  2. Summary of Recent Results from NASA's Space Solar Power (SSP) Programs and the Current Capabilities of Microwave WPT Technology

    Science.gov (United States)

    McSpadden, James; Mankins, John C.; Howell, Joe T. (Technical Monitor)

    2002-01-01

    The concept of placing enormous solar power satellite (SPS) systems in space represents one of a handful of new technological options that might provide large-scale, environmentally clean base load power into terrestrial markets. In the US, the SPS concept was examined extensively during the late 1970s by the U.S. Department of Energy (DOE) and the National Aeronautics and Space Administration (NASA). More recently, the subject of space solar power (SSP) was reexamined by NASA from 1995-1997 in the "fresh look" study, and during 1998 in an SSP "concept definition study". As a result of these efforts, in 1999-2000, NASA undertook the SSP Exploratory Research and Technology (SERT) program which pursued preliminary strategic technology research and development to enable large, multi-megawatt SSP systems and wireless power transmission (WPT) for government missions and commercial markets (in-space and terrestrial). During 2001-2002, NASA has been pursuing an SSP Concept and Technology Maturation (SCTM) program follow-on to the SERT, with special emphasis on identifying new, high-leverage technologies that might advanced the feasibility of future SSP systems. In addition, in 2001, the U.S. National Research Council (NRC) released a major report providing the results of a peer review of NASA's SSP strategic research and technology (R&T) road maps. One of the key technologies needed to enable the future feasibility of SSP/SPS is that of wireless power transmission. Advances in phased array antennas and rectennas have provided the building blocks for a realizable WPT system. These key components include the dc-RF converters in the transmitter, the retrodirective beam control system, and the receiving rectenna. Each subject is briefly covered, and results from the SERT program that studied a 5.8 GHz SPS system are presented. This paper presents a summary results from NASA's SSP efforts, along with a summary of the status of microwave WPT technology development.

  3. Preventive effects of Lactobacillus casei-14 on ulcerative colitis in mice%Lactobacillus casei-14对小鼠溃疡性结肠炎的预防作用

    Institute of Scientific and Technical Information of China (English)

    汪昕昕; 任倩然; 付天娇; 张卫兵; 任发政; 甘伯中

    2013-01-01

    采用2.5%右旋葡聚糖硫酸钠(DSS)诱导小鼠溃疡性结肠炎模型,研究Lactobacillus casei-14(L.casei-14)对小鼠溃疡性结肠炎(UC)的预防作用,并探讨其可能的机制.结果表明:L.casei-14高剂量组小鼠结肠长度为(7.66±0.58)cm,与模型组((6.73±0.42)cm)相比差异显著(P<0.05);中、高剂量组小鼠结肠炎疾病活动指数(DAI)分别为(4.22±0.97)和(4.17 ±1.33),与模型组(8.00±2.12)相比差异极显著(P<0.01);组织病理学炎症评分结果显示,中、高剂量组小鼠炎症评分分别为(4.88±0.83)和(4.13±0.64),极显著低于模型组(10.50±1.85)(P<0.01);中、高剂量组小鼠IL-1β、TNF-α mRNA表达水平均显著低于模型组,与正常对照组没有极显著性差异(P<0.01).说明L.casei-14对小鼠溃疡性结肠炎具有预防作用,其机制可能是通过抑制IL-1β和TNF-α炎症细胞因子的表达而减轻小鼠结肠黏膜的损伤,以达到预防UC的目的.

  4. Lactobacillus casei Exerts Anti-Proliferative Effects Accompanied by Apoptotic Cell Death and Up-Regulation of TRAIL in Colon Carcinoma Cells.

    Directory of Open Access Journals (Sweden)

    Angeliki Tiptiri-Kourpeti

    Full Text Available Probiotic microorganisms such as lactic acid bacteria (LAB exert a number of strain-specific health-promoting activities attributed to their immunomodulatory, anti-inflammatory and anti-carcinogenic properties. Despite recent attention, our understanding of the biological processes involved in the beneficial effects of LAB strains is still limited. To this end, the present study investigated the growth-inhibitory effects of Lactobacillus casei ATCC 393 against experimental colon cancer. Administration of live Lactobacillus casei (as well as bacterial components thereof on murine (CT26 and human (HT29 colon carcinoma cell lines raised a significant concentration- and time-dependent anti-proliferative effect, determined by cell viability assays. Specifically, a dramatic decrease in viability of colon cancer cells co-incubated with 10(9 CFU/mL L. casei for 24 hours was detected (78% for HT29 and 52% for CT26 cells. In addition, live L. casei induced apoptotic cell death in both cell lines as revealed by annexin V and propidium iodide staining. The significance of the in vitro anti-proliferative effects was further confirmed in an experimental tumor model. Oral daily administration of 10(9 CFU live L. casei for 13 days significantly inhibited in vivo growth of colon carcinoma cells, resulting in approximately 80% reduction in tumor volume of treated mice. Tumor growth inhibition was accompanied by L. casei-driven up-regulation of the TNF-related apoptosis-inducing ligand TRAIL and down-regulation of Survivin. Taken together, these findings provide evidence for beneficial tumor-inhibitory, anti-proliferative and pro-apoptotic effects driven by this probiotic LAB strain.

  5. Lactobacillus casei Exerts Anti-Proliferative Effects Accompanied by Apoptotic Cell Death and Up-Regulation of TRAIL in Colon Carcinoma Cells.

    Science.gov (United States)

    Tiptiri-Kourpeti, Angeliki; Spyridopoulou, Katerina; Santarmaki, Valentina; Aindelis, Georgios; Tompoulidou, Evgenia; Lamprianidou, Eleftheria E; Saxami, Georgia; Ypsilantis, Petros; Lampri, Evangeli S; Simopoulos, Constantinos; Kotsianidis, Ioannis; Galanis, Alex; Kourkoutas, Yiannis; Dimitrellou, Dimitra; Chlichlia, Katerina

    2016-01-01

    Probiotic microorganisms such as lactic acid bacteria (LAB) exert a number of strain-specific health-promoting activities attributed to their immunomodulatory, anti-inflammatory and anti-carcinogenic properties. Despite recent attention, our understanding of the biological processes involved in the beneficial effects of LAB strains is still limited. To this end, the present study investigated the growth-inhibitory effects of Lactobacillus casei ATCC 393 against experimental colon cancer. Administration of live Lactobacillus casei (as well as bacterial components thereof) on murine (CT26) and human (HT29) colon carcinoma cell lines raised a significant concentration- and time-dependent anti-proliferative effect, determined by cell viability assays. Specifically, a dramatic decrease in viability of colon cancer cells co-incubated with 10(9) CFU/mL L. casei for 24 hours was detected (78% for HT29 and 52% for CT26 cells). In addition, live L. casei induced apoptotic cell death in both cell lines as revealed by annexin V and propidium iodide staining. The significance of the in vitro anti-proliferative effects was further confirmed in an experimental tumor model. Oral daily administration of 10(9) CFU live L. casei for 13 days significantly inhibited in vivo growth of colon carcinoma cells, resulting in approximately 80% reduction in tumor volume of treated mice. Tumor growth inhibition was accompanied by L. casei-driven up-regulation of the TNF-related apoptosis-inducing ligand TRAIL and down-regulation of Survivin. Taken together, these findings provide evidence for beneficial tumor-inhibitory, anti-proliferative and pro-apoptotic effects driven by this probiotic LAB strain. PMID:26849051

  6. Expression of cbsA Encoding the Collagen-Binding S-Protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T

    OpenAIRE

    Martínez, Beatriz; Sillanpää, Jouko; Smit, Egbert,; Korhonen, Timo K.; Pouwels, Peter H.

    2000-01-01

    The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens.

  7. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on the substantiation of a health claim related to Lactobacillus rhamnosus GG and maintenance of normal defecation during antibiotic treatment pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    claim related to Lactobacillus rhamnosus GG and maintenance of normal defecation during antibiotic treatment. The food constituent, Lactobacillus rhamnosus GG, which is the subject of the health claim, is sufficiently characterised. The claimed effect proposed by the applicant is “help to maintain...... human intervention studies, from which conclusions could be drawn for the scientific substantiation of the claim, did not show an effect of Lactobacillus rhamnosus GG on the incidence of diarrhoea resulting from antibiotic treatment. The Panel concludes that a cause and effect relationship has not been...... established between the consumption of Lactobacillus rhamnosus GG and maintenance of normal defecation during antibiotic treatment. © European Food Safety Authority, 2013...

  8. Heterologous Expression of Mannanase and Developing a New Reporter Gene System in Lactobacillus casei and Escherichia coli.

    Science.gov (United States)

    Lin, Jinzhong; Zou, Yexia; Ma, Chengjie; She, Qunxin; Liang, Yunxiang; Chen, Zhengjun; Ge, Xiangyang

    2015-01-01

    Reporter gene systems are useful for studying bacterial molecular biology, including the regulation of gene expression and the histochemical analysis of protein products. Here, two genes, β-1,4-mannanase (manB) from Bacillus pumilus and β-glucuronidase (gusA) from Escherichia coli K12, were cloned into the expression vector pELX1. The expression patterns of these reporter genes in Lactobacillus casei were investigated by measuring their enzymatic activities and estimating their recombinant protein yields using western blot analysis. Whereas mannanase activity was positively correlated with the accumulation of ManB during growth, GusA activity was not; western blot analysis indicated that while the amount of GusA protein increased during later growth stages, GusA activity gradually decreased, indicating that the enzyme was inactive during cell growth. A similar trend was observed in E. coli JM109. We chose to use the more stable mannanase gene as the reporter to test secretion expression in L. casei. Two pELX1-based secretion vectors were constructed: one carried the signal peptide of the unknown secretion protein Usp45 from Lactococcus lactis (pELSH), and the other contained the full-length SlpA protein from the S-layer of L. acidophilus (pELWH). The secretion of ManB was detected in the supernatant of the pELSH-ManB transformants and in the S-layer of the cell surface of the pELWH-ManB transformants. This is the first report demonstrating that the B. pumilus manB gene is a useful reporter gene in L. casei and E.coli. PMID:26562012

  9. EFFECTS OF PROBIOTIC Lactobacillus casei Zhang ON THE EXPRESSION OF iNOS/NO IN RAW264.7 CELLS AND RELATED MECHANISMS%益生菌Lactobacillus Casei Zhang对巨噬细胞中NO/iNOS表达的影响及其机制

    Institute of Scientific and Technical Information of China (English)

    张威; 谢基明; 云小乐; 董仕超; 王玉珍; 张和平

    2014-01-01

    目的:研究益生菌L Casei Zhang对巨噬细胞RAW264.7中NO/iNOS表达的影响及探讨相关机制.方法:1×106CFU益生菌LCasei Zhang与巨噬细胞RAW264.7细胞共培养2h,6h,12h,24h.收集细胞培养上清,检测NO的表达变化.通过Real-rime PCR检测iNOS、TLR2和TLR4表达的变化.L Casei Zhang与RAW264.7共培养3h后,以LPS、CpG和PolyI:C刺激细胞,检测NO和iNOS表达的变化.结果:益生菌L Casei Zhang促进了巨噬细胞中NO分泌和iNOS的表达.与TLR2的本底表达相比,LCasei Zhang上调了RAW264.7细胞中TLR2的表达.TLR4 mRNA的表达在厶Casei Zhang与RAW264.7细胞共培养后2h显著增高.LPS和PolyI:C活化的巨噬细胞在L Casei Zhang预处理后,NO/iNOS的表达水平显著降低.结论:益生菌L Casei Zhang促进巨噬细胞中NO/iNOS的表达与促进TLR2和TLR4的表达有关.L Casei Zhang抑制了LPS和PolyI:C诱导的巨噬细胞中NO/iNOS的表达.该研究为进一步阐明益生菌的免疫调节作用奠定了基础.

  10. Antimicrobial Activity of Intraurethrally Administered Probiotic Lactobacillus casei in a Murine Model of Escherichia coli Urinary Tract Infection

    OpenAIRE

    Asahara, Takashi; Nomoto, Koji; Watanuki, Masaaki; Yokokura, Teruo

    2001-01-01

    The antimicrobial activity of the intraurethrally administered probiotic Lactobacillus casei strain Shirota against Escherichia coli in a murine urinary tract infection (UTI) model was examined. UTI was induced by intraurethral administration of Escherichia coli strain HU-1 (a clinical isolate from a UTI patient, positive for type 1 and P fimbriae), at a dose of 1 × 106 to 2 × 106 CFU in 20 μl of saline, into a C3H/HeN mouse bladder which had been traumatized with 0.1 N HCl followed immediate...

  11. Eruca sativa Might Influence the Growth, Survival under Simulated Gastrointestinal Conditions and Some Biological Features of Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus Strains

    Directory of Open Access Journals (Sweden)

    Florinda Fratianni

    2014-10-01

    Full Text Available The growth and viability of three Lactobacillus strains, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus, after their passage through simulated gastric and pancreatic juices were studied as a function of their presence in the growth medium of rocket salad (Eruca sativa. The presence of E. sativa affected some of the biological properties of the strains. For example, L. acidophilus and L. plantarum worked more efficiently in the presence of E. sativa, increasing not only the antioxidant activity of the medium, but also their own antioxidant power and antimicrobial activity; L. rhamnosus was not affected in the same manner. Overall, the presence of vegetables might help to boost, in specific cases, some of the characteristics of lactobacilli, including antioxidant and antimicrobial power.

  12. Eruca sativa might influence the growth, survival under simulated gastrointestinal conditions and some biological features of Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus strains.

    Science.gov (United States)

    Fratianni, Florinda; Pepe, Selenia; Cardinale, Federica; Granese, Tiziana; Cozzolino, Autilia; Coppola, Raffaele; Nazzaro, Filomena

    2014-10-01

    The growth and viability of three Lactobacillus strains, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus, after their passage through simulated gastric and pancreatic juices were studied as a function of their presence in the growth medium of rocket salad (Eruca sativa). The presence of E. sativa affected some of the biological properties of the strains. For example, L. acidophilus and L. plantarum worked more efficiently in the presence of E. sativa, increasing not only the antioxidant activity of the medium, but also their own antioxidant power and antimicrobial activity; L. rhamnosus was not affected in the same manner. Overall, the presence of vegetables might help to boost, in specific cases, some of the characteristics of lactobacilli, including antioxidant and antimicrobial power.

  13. Human Origin Lactobacillus casei Isolated from Indonesian Infants Demonstrating Potential Characteristics as Probiotics in vitro

    Directory of Open Access Journals (Sweden)

    Widodo .

    2015-11-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} The aim of this experiment was to isolate and identify Lactic Acid Bacteria (LAB from infant faeces and subsequent evaluation of its potential probiotics. LAB was isolated from faeces of infants who consumed breast milk as the only source of diet on L-cysteine-supplemented MRS Agar, and incubated on 37oC for 48 hours. Colonies grew on this media were then identifi ed based on morphological, physiological and molecular approaches. Morphological and physiological identifi cations based on Gram staining, shape, motility, spore formation, catalase, CO2 and NH3 production, and the ability to grow on temperature at 10oC and 45oC. Molecular identifi cation based on the amplifi cation of 16S rRNA gene. The potential application of selected isolates for probiotics was evaluated based on the ability to grow on media with low pH and the addition of 0.5% bile salts, the ability to inhibit the growth of pathogenic Bacillus cereus and Eschericia coli, and in vitroadherence ability. On the basis of morphological, physiological and molecular analysis of 16S rRNA gene, it was concluded that the selected isolate 1AF was a strain of Lactobacillus casei. Evaluation of probiotic in vitro showed that 60.4% of cells were resistant

  14. Immunological evaluation of Lactobacillus casei Zhang: a newly isolated strain from koumiss in Inner Mongolia, China

    Directory of Open Access Journals (Sweden)

    Du Ruiting

    2008-11-01

    Full Text Available Abstract Background There is increasing evidence to suggest an immunomodulation function both within the intestines and systemically upon consuming probiotic species. We recently isolated a novel LAB, Lactobacillus caseiZhang (LcZhang from koumiss. LcZhang exhibited favorable probiotic properties, such as acid resistance, bile resistance, gastrointestinal (GI colonization ability, etc. In order to examine the immunomodulatory qualities of LcZhang, we administered LcZhang to healthy mice with varying doses of either live or heat-killed LcZhang and measured various parameters of the host immune response. Results The study was performed in four separate experiments via oral administration of live and heat-killed LcZhang to BALB/c mice for several consecutive days. We investigated the immunomodulating capacity of LcZhang in vivo by analyzing the profile of cytokines, T cell subpopulations, and immunoglobulin concentrations induced in blood serum and intestinal fluid in BALB/c mice. Only live bacteria elicited a wide range of immune responses, which include the increased production of interferon-γ (IFN-γ, and depression of tumor necrosis factor-α (TNF-α levels. In addition, interleukin-2 (IL-2 and IL-2 receptor gene transcription increased significantly, but the proportion of T cell subsets appeared to be unaffected. We also observed that LcZhang was capable of inducing gut mucosal responses by enhancing the production of secretory Immunoglobulin A (sIgA as well influencing the systemic immunity via the cytokines released to the circulating blood. Conclusion The present work shows that the dose-dependent administration of LcZhang is capable of influencing immune responses, implying that it may be a valuable strain for probiotic use in humans.

  15. Survival of the probiotic bacteria Lactobacillus rhamnosus in seawater and its bioencapsulation in the brine shrimp Artemia

    OpenAIRE

    Claudia Ofelio; Alex Paltrinieri

    2014-01-01

    The probiotic Lactobacillus rhamnosus IMC 501® (Synbiotec Srl, Camerino, Italy) has demonstrated a positive effect in growth and early development of some fish species as clownfish (Amphiprion ocellaris, Cuvier 1830) and zebrafish (Danio rerio, Hamilton-Buchanan, 1822). The probiotic effects in A. ocellaris were an increase in body weight, a metamorphosis and skeletal head development acceleration and a reduction in the response to general stress (Avella et al. 2010). Besides this, an acceler...

  16. Lipopolysaccharide-Induced Profiles of Cytokine, Chemokine, and Growth Factors Produced by Human Decidual Cells Are Altered by Lactobacillus rhamnosus GR-1 Supernatant

    OpenAIRE

    Li, Wei; Yang, Siwen; Kim, Sung O.; Reid, Gregor; Challis, John R. G.; Bocking, Alan D.

    2014-01-01

    The aim of this study was to assess the effects of bacterial lipopolysaccharide (LPS) and Lactobacillus rhamnosus GR-1 supernatant (GR-1SN) on secretion profiles of cytokines, chemokines, and growth factors from primary cultures of human decidual cells. Lipopolysaccharide significantly increased the output of proinflammatory cytokines (interleukin [IL]-1B, IL-2, IL-6, IL-12p70, IL-15, IL-17A, interferon gamma [IFN-γ], and tumor necrosis factor [TNF]); anti-inflammatory cytokines (IL-1RN, IL-4...

  17. Production of a heterologous nonheme catalase by Lactobacillus casei: an efficient tool for removal of H2O2 and protection of Lactobacillus bulgaricus from oxidative stress in milk.

    Science.gov (United States)

    Rochat, Tatiana; Gratadoux, Jean-Jacques; Gruss, Alexandra; Corthier, Gérard; Maguin, Emmanuelle; Langella, Philippe; van de Guchte, Maarten

    2006-08-01

    Lactic acid bacteria (LAB) are generally sensitive to H2O2, a compound that they can paradoxically produce themselves, as is the case for Lactobacillus bulgaricus. Lactobacillus plantarum ATCC 14431 is one of the very few LAB strains able to degrade H2O2 through the action of a nonheme, manganese-dependent catalase (hereafter called MnKat). The MnKat gene was expressed in three catalase-deficient LAB species: L. bulgaricus ATCC 11842, Lactobacillus casei BL23, and Lactococcus lactis MG1363. While the protein could be detected in all heterologous hosts, enzyme activity was observed only in L. casei. This is probably due to the differences in the Mn contents of the cells, which are reportedly similar in L. plantarum and L. casei but at least 10- and 100-fold lower in Lactococcus lactis and L. bulgaricus, respectively. The expression of the MnKat gene in L. casei conferred enhanced oxidative stress resistance, as measured by an increase in the survival rate after exposure to H2O2, and improved long-term survival in aerated cultures. In mixtures of L. casei producing MnKat and L. bulgaricus, L. casei can eliminate H2O2 from the culture medium, thereby protecting both L. casei and L. bulgaricus from its deleterious effects.

  18. Inactivation of Escherichia coli in broth and sausage by combined high pressure and Lactobacillus casei cell extract.

    Science.gov (United States)

    Chung, Hyun-Jung; Yousef, Ahmed E

    2010-10-01

    The purpose of this study was to investigate the effect of combined high pressure and Lactobacillus casei cell extract (CE) on Escherichia coli O157 strains with variation in pressure resistance in broth and sausage. Pressure-resistant (O157:H7 and O157:H12) and -sensitive (O157-M1 and O157-M2) E. coli strains were used. Pressure treatment at 350 MPa for 20 min in broth caused 1.1-1.2 logs reduction in O157:H12 and O157:H7 and 4.1-5.5 logs reduction in the O157-M1 and O157-M2. When high pressure was treated in the presence of CE (32 CEAU/mL), the combination treatment caused a significant inactivation in the pressure-resistant O157:H7 strains resulting in the viability loss of 4.3-4.6 logs and the synergistic effect increased with increase in treatment time (p high pressure treatment. The synergy between high pressure processing and Lb. casei OSY-LB6A CE against pressure-resistant E. coli O157 strains suggests the feasibility of using this combination to minimize the risk of transmission of E. coli O157 by food.

  19. Nutritional requirements for methyl orange decolourisation by freely suspended cells and growing cells of Lactobacillus casei TISTR 1500

    Directory of Open Access Journals (Sweden)

    Phisit Seesuriyachan

    2011-01-01

    Full Text Available Lactobacillus casei TISTR 1500 possesses cytoplasmic azoreductase and can breakdown azo bonds under microaerophilic condition. It was found previously that a growing culture is more tolerant to a high initial dye concentration than freely suspended cells supplied only with sucrose. The present study is aimed at investigating the nutritive requirements for decolourisation by the growing cells and the freely suspended cells using Plackett-Burmann experimental design. In this study, the composition of the medium was found to play an important role in methyl orange decolourisation and biomass production. Sucrose, meat extract and peptone increased methyl orange decolourisation by freely suspended cells, whereas sodium acetate exerted a negative effect on decolourisation. In addition, it was observed that the yeast and meat extracts enhanced the degradation of the dye by the growing cells. Sucrose was an important factor in biomass production by freely suspended cells and growing cells. On the other hand, dipotassium hydrogen phosphate and sodium acetate decreased the biomass production. These findings promote the understanding and knowledge about the requirements of azo dye decolourisation by Lactobacillus casei.

  20. Free and immobilized Lactobacillus casei ATCC 393 on whey protein as starter cultures for probiotic Feta-type cheese production.

    Science.gov (United States)

    Dimitrellou, Dimitra; Kandylis, Panagiotis; Sidira, Marianthi; Koutinas, Athanasios A; Kourkoutas, Yiannis

    2014-01-01

    The use of free and immobilized Lactobacillus casei ATCC 393 on whey protein as starter culture in probiotic Feta-type cheese production was evaluated. The probiotic cultures resulted in significantly higher acidity; lower pH; reduced counts of coliforms, enterobacteria, and staphylococci; and improved quality characteristics compared with cheese with no culture. Microbiological and strain-specific multiplex PCR analysis showed that both free and immobilized L. casei ATCC 393 were detected in the novel products at levels required for conferring a probiotic effect at the end of the ripening. The effect of starter culture on production of volatile compounds was investigated by the solid-phase microextraction gas chromatography-mass spectrometry analysis technique. The immobilized cells resulted in an improved profile of aroma-related compounds and the overall high quality of the novel products was ascertained by the preliminary sensory test. Finally, the high added value produced by exploitation of whey, which is an extremely polluting industrial waste, was highlighted and assessed. PMID:24931523

  1. SSP Technology Investigation of a High-Voltage DC-DC Converter

    Science.gov (United States)

    Pappas, J. A.; Grady, W. M.; George, Patrick J. (Technical Monitor)

    2002-01-01

    The goal of this project was to establish the feasibility of a high-voltage DC-DC converter based on a rod-array triggered vacuum switch (RATVS) for the Space Solar Power system. The RATVS has many advantages over silicon and silicon-carbide devices. The RATVS is attractive for this application because it is a high-voltage device that has already been demonstrated at currents in excess of the requirement for an SSP device and at much higher per-device voltages than existing or near-term solid state switching devices. The RATVS packs a much higher specific power rating than any solid-state device and it is likely to be more tolerant of its surroundings in space. In addition, pursuit of an RATVS-based system would provide NASA with a nearer-term and less expensive power converter option for the SSP.

  2. Lactic acid production by mixed cultures of Kluyveromyces marxianus, Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus helveticus.

    Science.gov (United States)

    Plessas, S; Bosnea, L; Psarianos, C; Koutinas, A A; Marchant, R; Banat, I M

    2008-09-01

    Lactic acid production using Kluyveromyces marxianus (IFO 288), Lactobacillus delbrueckii ssp. bulgaricus (ATCC 11842) and Lactobacillus helveticus (ATCC 15009) individually or as mixed culture on cheese whey in stirred or static fermentation conditions was evaluated. Lactic acid production, residual sugar and cell biomass were the main features examined. Increased lactic acid production was observed, when mixed cultures were used in comparison to individual ones. The highest lactic acid concentrations were achieved when K. marxianus yeast was combined with L. delbrueckii ssp. bulgaricus, and when all the strains were used revealing possible synergistic effects between the yeast and the two lactic acid bacteria. The same synergistic effects were further observed and verified when the mixed cultures were applied in sourdough fermentations, proving that the above microbiological system could be applied in the food fermentations where high lactic acid production is sought.

  3. ARE ENTEROCOCCUS FAECIUM CRL 183 AND LACTOBACILLUS HELVETICUS SSP. JUGURTI 416 ABLE TO PRODUCE ANTIMICROBIAL SUBSTANCE?

    Directory of Open Access Journals (Sweden)

    R. BEDANI

    2008-12-01

    Full Text Available

    Production of antimicrobial substance by E. faecium CRL 183 and L. helveticus ssp jugurti 416 was tested against pathogenic bacteria and microorganisms isolated from Wistar rat faeces, using a spot-on-the-lawn assay. Three pathogenic bacteria (Listeria monocytogenes 1/2a, Salmonella enteridites 193/95, Escherichia coli O157:H7 and twenty-fi ve colonies, isolated from animal faeces, belonging to the following groups Enterococcus, Lactobacillus, Clostridium, Bacteroides and Enterobacteriaceae, were used as indicator microorganisms. The results showed that E. faecium CRL 183 and L. helveticus ssp jugurti 416 did not produce signifi cant antimicrobial activity against the indicator microorganisms tested. More tests needed to be carried out with other indicator bacteria and other culture media to confi rm the lack of antimicrobial production.

  4. Chemical Composition and Antimicrobial Activity of Thymus praecox Opiz ssp. polytrichus Essential Oil from Serbia

    Directory of Open Access Journals (Sweden)

    Nada V. Petrović

    2016-03-01

    Full Text Available Chemical composition and antimicrobial activity of the essential oil of wild growing Thymus praecox Opiz ssp. polytrichus were studied. trans-Nerolidol (19.79%, germacrene D (18.48% and thymol (9.62% were the main components in essential oil. This study is the first report of the antimicrobial activity of essential oil obtained from the T. praecox Opiz ssp. polytrichus. Antimicrobial activity of essential oil was investigated on Bacillus cereus, Micrococcus flavus, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Salmonella typhimurium, Aspergillus fumigatus, A. versicolor, A. ochraceus, A. niger, Trichoderma viride, Penicillium funiculosum, P. ochrochloron, and P. verrucosum var. cyclopium strains. In the antimicrobial assays, essential oil showed high antimicrobial potential (MIC 19–150 m g/mL, MBC 39–300 m g/mL for bacteria; and MIC 19.5–39 m g/mL, MFC 39–78 m g/mL for fungi.

  5. Susceptibility of wild carrot (Daucus carota ssp. carota) to Sclerotinia sclerotiorum

    DEFF Research Database (Denmark)

    Jensen, Brita Dahl; Finckh, M.R.; Munk, Lisa;

    2008-01-01

    Sclerotinia soft rot, caused by Sclerotinia sclerotiorum, is a severe disease of cultivated carrots (Daucus carota ssp. sativus) in storage. It is not known whether Sclerotinia soft rot also affects wild carrots (D. carota ssp. carota), which hybridise and exchange genes, among them resistance...... genes, with the cultivated carrot. We investigated the susceptibility of wild carrots to S. sclerotiorum isolates from cultivated carrot under controlled and outdoor conditions. Inoculated roots from both wild and cultivated plants produced sclerotia and soft rot in a growth chamber test. Two isolates...... differed significantly in the ability to produce lesions and sclerotia on roots of both wild carrots and cv. Bolero. Flowering stems of wild carrots produced dry, pale lesions after inoculation with the pathogen, and above-ground plant weight was significantly reduced 4 weeks after inoculation...

  6. Transport of Amino Acids in Lactobacillus casei by Proton-Motive-Force-Dependent and Non-Proton-Motive-Force-Dependent Mechanisms

    NARCIS (Netherlands)

    Strobel, Herbert J.; Russell, James B.; Driessen, Arnold J.M.; Konings, Wilhelmus

    1989-01-01

    Lactobacilus casei 393 cells which were energized with glucose (pH 6.0) took up glutamine, asparagine, glutamate, aspartate, leucine, and phenylalanine. Little or no uptake of several essential amino acids (valine, isoleucine, arginine, cysteine, tyrosine, and tryptophan) was observed. Inhibition st

  7. Use of Lactobacillus acidophilus and Lactobacillus casei for a potential probiotic legume-based fermented product using pigeon pea (Cajanus cajan).

    Science.gov (United States)

    Parra, K; Ferrer, M; Piñero, M; Barboza, Y; Medina, L M

    2013-02-01

    The aim of the present study was to evaluate the use of pigeon pea (Cajanus cajan) as an appropriate substrate in the production of a legume-based fermented product with Lactobacillus acidophilus ATCC 314 or Lactobacillus casei ATCC 393 and then to ascertain the effects of the addition of ingredients such as powdered milk and banana or strawberry sauce. The products were analyzed for viable cell counts, pH, and sensory attributes during product manufacture and throughout the refrigerated storage period at 3, 7, 14, 21, and 28 days. Nine types of products were produced. At the end of the storage period, the viability of L. acidophilus was above 7 log CFU/g in the presence of milk and 20% sucrose fruit sauce. For products with L. casei, the lack of ingredients such as milk caused no significant loss in viability; however, a high concentration of sucrose in the fruit sauce was an important factor in maintaining a high L. casei population. L. casei had high viability and good sensory attributes. Both strains could be considered suitable for a pigeon pea-based fermented potential probiotic product and a low-cost protein source. PMID:23433374

  8. Expression of Lactobacillus reuteri Pg4 collagen-binding protein gene in Lactobacillus casei ATCC 393 increases its adhesion ability to Caco-2 cells.

    Science.gov (United States)

    Hsueh, Hsiang-Yun; Yueh, Pei-Ying; Yu, Bi; Zhao, Xin; Liu, Je-Ruei

    2010-12-01

    The collagen-binding protein gene cnb was cloned from the probiotic Lactobacillus reuteri strain Pg4. The DNA sequence of the cnb gene (792 bp) has an open reading frame encoding 263 amino acids with a calculated molecular weight of 28.5 kDa. The cnb gene was constructed so as to constitutively express under the control of the Lactococcus lactis lacA promoter and was transformed into Lactobacillus casei ATCC 393, a strain isolated from dairy products with poor ability to adhere to intestinal epithelial cells. Confocal immunofluorescence microscopic and flow cytometric analysis of the transformed strain Lb. casei pNZ-cnb indicated that Cnb was displayed on its cell surface. Lb. casei pNZ-cnb not only showed a higher ability to adhere to Caco-2 cells but also exhibited a higher competition ability against Escherichia coli O157:H7 and Listeria monocytogenes adhesion to Caco-2 cells than Lb. casei ATCC 393. PMID:21070005

  9. Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T

    NARCIS (Netherlands)

    Martínez, B.; Sillanpää, J.; Smit, E.; Korhonen, T.K.; Pouwels, P.H.

    2000-01-01

    The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting sig

  10. SSP: a simple software process for small- size software development projects

    OpenAIRE

    Ochoa, Sergio; Pino, José A.; Luis A. Guerrero; Cesar A Collazos

    2006-01-01

    A large number of software development projects in Latin- American countries are small-size, poorly defined and time pressured. These projects usually involve under qualified people. Provided that well-known software development models have shown limited applicability in such scenario, developers usually carry out ad-hoc software processes. Therefore, the obtained results are unpredictable. This article presents a Simple Software Process (SSP) for small-size software projects involving under ...

  11. Evaluating the Strange Situation Procedure (SSP to assess the bond between dogs and humans.

    Directory of Open Access Journals (Sweden)

    Therese Rehn

    Full Text Available The Strange Situation Procedure (SSP is increasingly being used to study attachment between dogs and humans. It has been developed from the Ainsworth Strange Situation Procedure, which is used extensively to investigate attachment between children and their parents. In this experiment, 12 female beagle dogs were tested in two treatments to identify possible order effects in the test, a potential weakness in the SSP. In one treatment (FS, dogs participated together with a 'familiar person' and a 'stranger'. In a control treatment (SS, the same dogs participated together with two unfamiliar people, 'stranger A' and 'stranger B'. Comparisons were made between episodes within as well as between treatments. As predicted in FS, dogs explored more in the presence of the familiar person than the stranger. Importantly, they also explored more in the presence of stranger A (who appeared in the same order as the familiar person and followed the same procedure than stranger B in SS. Furthermore, comparisons between treatments, where a familiar person was present in FS and stranger A was present in SS, showed no differences in exploration. In combination, these results indicate that the effect of a familiar person on dogs' exploratory behaviour, a key feature when assessing secure attachment styles, could not be tested reliably due to the order in which the familiar person and the stranger appear. It is proposed that in the future only counterbalanced versions of the SSP are used. Alternatively, since dogs reliably initiated more contact with the familiar person compared to the strangers, it is suggested that future studies on attachment in dogs towards humans should focus either on the behaviour of the dog in those episodes of the SSP when the person returns, or on reunion behaviour in other studies, specially designed to address dog-human interactions at this time.

  12. Brain SPECT analysis by 3D-SSP and clinical features of Parkinson's disease

    International Nuclear Information System (INIS)

    The aim of the present study is to investigate the association of symptoms in Parkinson's disease (PD) with cerebral perfusion on single photon emission computed tomography (SPECT). The clinical features of PD were compared with SPECT images of the brain obtained by three-dimensional stereotactic surface projection (3D-SSP) analysis. Thirty-eight patients who had PD without dementia (17 men and 21 women with a mean age of 68.6±4.7 years) were enrolled in this study. Their symptoms were rated using the unified parkinson's disease rating scale (UPDRS). Within a week, all patients were examined by SPECT with I-123, and reconstructed images were analyzed with 3D-SSP using an image-analysis software, iSSP ver. 3.5. Data on brain surface perfusion extracted by 3D-SSP analysis were compared between the PD patients and the normal control group. The same comparisons were made for subgroups of PD patients with severe symptoms, such as tremor, gait disturbance, bradykinesia, and the UPDRS motor score. Cerebral perfusion was decreased at the anterior cingulate cortex and occipital lobe of the PD patients compared with the normal controls. In the subgroups with severe gait disturbance and severe bradykinesia, additional hypoperfusion was seen at the lateral frontal association and lateral temporal association and the medial frontal gyrus, and by the pixel-by-pixel comparison, perfusion was significantly decreased (p<0.05) at the medial frontal gyrus and anterior cingulate cortex compared with the normal control group. In PD patients, severe gait disturbance and bradykinesia may be correlated with hypoperfusion of the medial aspect of the frontal lobe. This suggests that functional disturbance of the supplementary motor area and other parts of the frontal lobe are involved in the development of gait disturbance and bradykinesia in PD. (author)

  13. Khorasan wheat population researching (Triticum turgidum, ssp. Turanicum (McKey) in the minimum tillage conditions

    OpenAIRE

    Ikanović Jela; Popović Vera; Janković Snežana; Živanović Ljubiša; Rakić Sveto; Dončić Dalibor

    2014-01-01

    Khorasan wheat occupies a special place in the group of new-old cereals (Triticum turgidum, ssp. Turanicum McKey). It is an ancient species, native to eastern Persia, that is very close to durum wheat by morphological characteristics. Investigations were carried out in agro ecological conditions of the eastern Srem, with two wheat populations with dark and bright awns as objects of study. The following morphological and productive characteristics were inves...

  14. Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws.

    Directory of Open Access Journals (Sweden)

    Michael Marks

    Full Text Available Yaws, caused by Treponema pallidum ssp. pertenue, is a neglected tropical disease closely related to venereal syphilis and is targeted for eradication by 2020. Latent yaws represents a diagnostic challenge, and current tools cannot adequately distinguish between individuals with true latent infection and individuals who are serofast following successful treatment. PCR on blood has previously been shown to detect T. pallidum DNA in patients with syphilis, suggesting that this approach may be of value in yaws. We performed real-time PCR for Treponema pallidum ssp. pertenue on blood samples from 140 children with positive T. pallidum Particle Agglutination (TPPA and Rapid Plasma Reagin (RPR tests and 7 controls (negative serology, all collected as part of a prospective study of yaws in the Solomon Islands. All samples were also tested by a nested PCR for T. pallidum. 12 patients had clinical evidence of active yaws whilst 128 were considered to have latent yaws. 43 children had high titre rapid plasma reagins (RPRs of ≥1:32. PCR testing with both assays gave negative results in all cases. It is possible that the failure to detect T. pallidum ssp. pertenue in blood reflects lower loads of organism in latent yaws compared to those in latent infection with T. pallidum ssp. pertenue, and/or a lower propensity for haematogenous dissemination in yaws than in syphilis. As the goal of the yaws control programme is eradication, a tool that can differentiate true latent infection from individuals who are serofast would be of value; however, PCR of blood is not that tool.

  15. Incorporation of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium ssp.) in Argentinean ovine cheese

    OpenAIRE

    Perotti, María; Wolf, Irma; Addis, Margherita; Comunian, Roberta; Paba, Antonio; Meinardi, Carlos

    2014-01-01

    The market of cheeses from ewe’s milk has been growing steadily in Argentina. The nutritional benefits of these products can be enhanced by adding probiotic cultures. In the present study, the survival of a mix of probiotic microorganisms (Lactobacillus acidophilus La-5 and Bifidobacterium animalis ssp. lactis Bb12) in a semi-hard ovine cheese, manufactured with a technology previously developed in our institute was evaluated. Besides, the effect of its incorporation on the chemical compositi...

  16. Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws.

    Science.gov (United States)

    Marks, Michael; Katz, Samantha; Chi, Kai-Hua; Vahi, Ventis; Sun, Yongcheng; Mabey, David C; Solomon, Anthony W; Chen, Cheng Y; Pillay, Allan

    2015-01-01

    Yaws, caused by Treponema pallidum ssp. pertenue, is a neglected tropical disease closely related to venereal syphilis and is targeted for eradication by 2020. Latent yaws represents a diagnostic challenge, and current tools cannot adequately distinguish between individuals with true latent infection and individuals who are serofast following successful treatment. PCR on blood has previously been shown to detect T. pallidum DNA in patients with syphilis, suggesting that this approach may be of value in yaws. We performed real-time PCR for Treponema pallidum ssp. pertenue on blood samples from 140 children with positive T. pallidum Particle Agglutination (TPPA) and Rapid Plasma Reagin (RPR) tests and 7 controls (negative serology), all collected as part of a prospective study of yaws in the Solomon Islands. All samples were also tested by a nested PCR for T. pallidum. 12 patients had clinical evidence of active yaws whilst 128 were considered to have latent yaws. 43 children had high titre rapid plasma reagins (RPRs) of ≥1:32. PCR testing with both assays gave negative results in all cases. It is possible that the failure to detect T. pallidum ssp. pertenue in blood reflects lower loads of organism in latent yaws compared to those in latent infection with T. pallidum ssp. pertenue, and/or a lower propensity for haematogenous dissemination in yaws than in syphilis. As the goal of the yaws control programme is eradication, a tool that can differentiate true latent infection from individuals who are serofast would be of value; however, PCR of blood is not that tool. PMID:26125585

  17. Environmental Restoration and Waste Management Site-Specific Plan (SSP) for fiscal year 1992 (FY92)

    International Nuclear Information System (INIS)

    The FY-92 Site-Specific Plan (FY-92 SSP) for environmental restoration and waste management at the Idaho National Engineering Laboratory (INEL) is designed to provide the reader with easy access to the status of environmental restoration and waste management activities at INEL. The first chapter provides background on INIEL's physical environment, site history and mission, and general information about the site and its facilities. In addition, this chapter discusses the inter-relationships between the Site Specific Plan, the Environmental Restoration and Waste Management Five-Year Plan, the environmental restoration and waste management prioritization systems, and the Activity Data Sheets (ADSs) for environmental restoration and waste management. This discussion should help readers understand what the SSP is and how it fits into the environmental restoration and waste management process at INEL. This understanding should provide the reader with a better context for understanding the discussions in the SSP as well as a better feel for how and what to comment on during the public comment period that will be held from the first of September through the end of October 1991

  18. Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida.

    Science.gov (United States)

    Mancuso, Monique; Avendaño-Herrera, Rubén; Zaccone, R; Toranzo, Alicia E; Magariños, Beatriz

    2007-01-01

    This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections. PMID:17657358

  19. Simultaneous genotyping of HPA-17w to -21w by PCR-SSP in Chinese Cantonese.

    Science.gov (United States)

    Zhou, Haojie; Ding, Haoqiang; Chen, Yangkai; Li, Xiaofan; Ye, Xin; Nie, Yongmei

    2015-01-01

    Studies have reported the polymorphism of human platelet antigen (HPA)-17w, -18w, -19w, -20w, and -21w. However, the distribution of these five antigens in Chinese Cantonese is still unknown. In this study, we designed new sequence-specific primers for HPA-19w to -21w and used published primers for HPA-17w and -18w to develop a polymerase chain reaction with the sequence-specific primers (PCR-SSP) method for simultaneously genotyping HPA-17w to -21w. A total of 820 unrelated Cantonese apheresis platelet donors in Guangzhou were involved in this study. Among the five HPAs, complete a/a homozygosity was observed for HPA-17w to -20w with an allele frequency of 1.0000. For HPA-21w, nine individuals (9/820, 1.10%) were found to be HPA-21a/bw heterozygous and the allele frequencies of HPA-21a and HPA-21bw were 0.9945 (1631/1640) and 0.0055 (9/1640), respectively. The reliability of the PCR-SSP method was determined by comparing with the genotyping results by DNA sequencing, and no inconsistencies were observed between the two methods. This study provides a reliable PCR-SSP method for simultaneously genotyping HPA-17w to -21w and could improve HPA-matched platelet transfusion in Chinese Cantonese.

  20. The catalase gene differentiates between some strains of Staphylococcus aureus ssp. anaerobius.

    Science.gov (United States)

    Musa, N O; Eltom, K; Gessler, F; Böhnel, H; Babiker, A; El Sanousi, S M

    2010-05-01

    Staphylococcus aureus ssp anaerobius strain S10 was isolated from an outbreak of sheep abscess disease. Sequence of the catalase gene of this strain showed 99% identity to the catalase gene (katB) sequence of the reference strain (S. aureus ssp. anaerobius strain MVF213) with mismatching of three base pairs. An important substitution located 1036 nucleotides upstream of the initiation codon from "C" in katB to "T" in the catalase gene of strain S10 originated a stop codon. The deduced protein (345 amino acids) is 105 amino acids shorter than that of katB. Partial sequence of the catalase gene of other 8 local isolates in addition to another reference strain (DSM 20714/ATCC 35844) revealed the same mutations in all local (African) strains, whereas the sequence of the reference (European) strain was typical to that of katB. Sequence of the catalase gene of S. aureus ssp. anaerobius strain S10 was deposited in GenBank under accession no. EU281993. PMID:20526831

  1. 益生菌Lactobacillus casei Zhang对凝固型发酵乳质构和挥发性风味物质的影响%Effects of Probiotic Lactobacillus casei Zhang on the Texture Profile and Volatile Compounds of Set Yogurt

    Institute of Scientific and Technical Information of China (English)

    其木格苏都; 郭壮; 王记成; 孟和毕力格; 张和平

    2013-01-01

    [目的]评价益生菌Lactobacillus casei Zhang(L.casei Zhang)货架期内活菌数的变化及其对发酵乳物理特性和挥发性风味物质的影响.[方法]将2株Streptococcus thermophilus(S.thermophilus)分别与益生菌L.casei Zhang复配发酵,发酵结束(pH 4.5)于4℃冷藏28 d,并对发酵乳各指标进行测定.[结果]4℃贮藏28 d时,发酵乳中益生菌L.casei Zhang活菌数没有显著性变化(P<0.05),且数量保持在108CFU/g以上;益生菌L.casei Zhang与S.thermophilus复配发酵有利于提高3-羟基-2-丁酮和己酸等挥发性风味物质的含量,同时短期贮藏过程中(1-7 d)可赋予产品较高的黏度、硬度、脆度、胶性和耐嚼性,随着贮藏时间的延长其对产品物理特性的影响随发酵用S.thermophilus菌株的不同而呈现不同的变化.[结论]4℃贮藏28 d过程中,益生菌L.casei Zhang具有非常好的稳定性,同时其与S.thermophillus复配发酵可赋予产品较好的质构和风味.%[Objective] The viability of the probiotic Lactobacillus casei Zhang during the product sbelf-life and its effects on the texture profile and the production of volatile compounds of fermented milk were studied.[Method] L.casei Zhang was inoculated together with either of the two Streptococcus thermophilus strains into pasteurized milk for fermentation,the fermentation was ended when the pH value of fermented milk reached 4.5,then,specific indexes of the products were determined after storage at 4℃C for 28 d.[Result] The viable count ofL.casei Zhang was highly stable (P<0.05),and was more than 108CFU/g aRer storage at 4℃ for 28 d.Furthermore,by simultaneous inoculating L.casei Zhang and the selected strains ofS.thermophilus into the milk at a certain proportion,it was beneficial to improve the content of 3-hydroxyl-2-butanone and hexylic acid in products; this method also enhanced the viscosity,hardness,fracturability,gumminess and chewiness of the products during short-term storage (1-7 d

  2. EFFECT OF CULTURE MEDIUM ON BACTERIOCIN PRODUCTION BY LACTOBACILLUS RHAMNOSUS HN001 AND LACTOBACILLUS REUTERI ATCC 53608

    Directory of Open Access Journals (Sweden)

    Aguilar-Uscanga B. R.

    2013-06-01

    Full Text Available The aim of this study was to evaluate the effect of media on bacteriocin production by Lactobacillus rhamnosus HN001 and Lactobacillus reuteri ATCC 53608 using three different media: YPM, YPF and MRS supplemented with glucose and K2HPO4. The optimum temperature was 37°C and initial pH 6.5. Bacteriocin-like substances produced by tested bacteria in MRS medium supplemented with glucose and K2HPO4 exhibited a broad antimicrobial spectrum determined by well diffusion assay against indicator bacteria Listeria monocytogenes, Lactobacillus sakei, Enterococcus faecium, Lactobacillus delbrueckii, Lactobacillus acidophilus, but no antimicrobial spectrum against E. coli O157:H7, Salmonella typhimurium, Staphylococcus aureus, Bacillus cereus was detected. Bacteriocin was sensitive to protease IV, trypsin, pepsin and -amylases, but resistant to lipase. It was also resistant to detergents such as Tween 80, Triton-X and SDS. This bacteriocin was thermo-stable (resistant at 60°C, 90°C and 100°C for 30 min. Tested bacteria showed the best antimicrobial (bacteriocin-like activity after growth in MRS medium. Bacteriocin substances produced by tested bacteria showed promising thermo-stable technological properties.

  3. Influence of manufacturing processes on in vitro properties of the probiotic strain Lactobacillus rhamnosus Lcr35®.

    Science.gov (United States)

    Nivoliez, Adrien; Camares, Olivier; Paquet-Gachinat, Marylise; Bornes, Stéphanie; Forestier, Christiane; Veisseire, Philippe

    2012-08-31

    Probiotics are administered as complex manufactured products and yet most studies on probiotic bacterial strains have been performed with native culture strains. Little is known about the influence of industrial processes on the properties of the microorganisms. In this study, we comparatively assessed the characteristics of the probiotic bacterial strain Lactobacillus rhamnosus (Lcr35(®)) together with four of its commercial formulations, including three intestinal formulas (BACILOR with Lcr Restituo(®) packet and capsule and FLOREA Lcr Lenio(®)) and one vaginal formula (GYNOPHILUS Lcr Regenerans(®)). Lcr35(®) grown from the intestinal formulas displayed increased resistance to acidic pH and bile stress, especially FLOREA (Lcr Lenio(®)), which showed a 4.5log higher number of viable bacteria compared to the results obtained with the control native Lcr35(®) strain. Adhesion to intestinal cells was significantly higher with Lcr Restituo(®) packet and Lcr Restituo(®) capsule vs Lcr35(®). Bacteria from the vaginal formulation GYNOPHILUS had increased ability to metabolize glycogen thereby increasing lactic acid production. In vitro growth inhibition of the pathogen Candida albicans was significantly higher with bacteria from the vaginal formulation (4.5 log difference) and in the presence of vaginal epithelial cells than with the native strain. Our results show that the manufacturing process influences strain properties and should therefore be adapted according to the strain and the therapeutic indication. PMID:22542933

  4. 益生乳酸菌Lactobacillus casei Zhang从基础研究到产业化开发%Probitotic Lactobacillus casei Zhang from basic research to industrialization

    Institute of Scientific and Technical Information of China (English)

    张和平

    2011-01-01

    2001年-2009年,内蒙古农业大学“乳品生物技术与工程”教育部重点实验室从采集自中国西部7个省市及蒙古国14个省市不同少数民族地区951份自然发酵乳制品等样品中分离鉴定出3 388林乳酸菌,建立了中国首个原创性乳酸菌菌种资源库;从菌种库中分离自传统发酵酸马奶(koumiss)的243株乳杆菌中筛选获得1株性能优良的乳酸菌-Lactobacillus casei Zhang(Lcasei Zhang).采用5种动物模型和人体试验进行了功能评价,并利用基因组学和蛋白质组学研究技术对L.casei Zhang益生分子机制进行了深入剖析.经过多年系统的基础研究,自主解决了其产业化的关键技术问题,包括其直投式发酵剂、发酵乳制品和发酵豆乳益生菌饮料的研发并实现了产业化.%During 2001-2009, we have isolated ,identified and collected 3, 388 strains of lactic add bacteria from 951 naturally fermented dairy products (including fermented cow milk, mare milk, goat milk, camel milk and yak milk) in Inner Mongolia, Xinjiang, Qinghai, Tibet, Yunnan ,Ganshu and Sichuan of China and 17 provinces of Mongolia, and established the first LAB Collection Center (LABCC) with self-owned intellectual property in China. From 243 strains of lactobaclli isolated from naturally fermented mare milk (koumiss) collected in LABCC , a novel probiotic-Lactobadllus casei Zhang was screened out,and conducted extensive researches on it .including acid tolerance and bile resistance to the gastointestinal digestion, adhesion to Coco-2 human epithelial cells line in vitro, immunomodulatory properties ,anti-tumor effects for H22 cancer cell line induced liver tumor , hypocholesterolemic effects, antagonistic to pathogenic Escherichia coli in intestine, anti-oxidative effect in vivo using mice or rat as models,and colonization of in human gut and regulation of gut microbiota for 24 volunteers. Using gemomics and proteomics ,we elucidated its probiotic mechanism partially

  5. 益生乳酸菌Lactobacillus casei Zhang和Lactobacillus plantarum P8对全价饲料pH及微生物类群变化的研究%Beneficial Lactobacillus casei and Lactobacillus plantarum P8 on the Influence and Changes of pH and Microbial Groups in Complete Feed

    Institute of Scientific and Technical Information of China (English)

    魏爱彬; 于洁; 王鑫; 王宏梅; 白娜; 张和平

    2012-01-01

    The influence of changes of Lactobacillus casei and Laclobacillus pla.ntaru.ni P8 on pH and microbial groups in complete feed were investigated. L. casei and L. plantarum P8 were inoculated alone or mixed (1:1) with 6.30 Ig cfu/g of total inoculation volume to ferment complete feed. The changes of pH and microbial groups in complete feed were monitored during ten days fermentation at 25℃. The dynamic changes of lactobacteria and sundry microbes ( yeasts, moulds, E. coli. Bacillus, and Clostridium) were determined using selective media; as well as the dynamic changes of L casei and L. planlarum PS with RT-PCR. The results showed that the pH of the tested groups decreased noticeably, 10 days after the fermentation, the pH of fermented feed that inoculated with L casei and L. planlarum P8 aione and mixed were at 4,23 , 4. 24 and 4, 22, respectively, significantly lower (P<0. 05 ) lhaa the control groups. The active numbers of i. casei and L. plantarum P8 in fermented feed with single and mixed were S. 91, 8.89 lg, 6. 58, and 8.69 lg cfu/g respectively. During the fermentation, the active number of yeasts, moulds, E. coli, Bacillus, and Closlridium were significantly lower than the control group (P <0.05). Among them the inhibition against sundry rai-erobes fermented with L. plantarum alone significantly outwent L. casei. Therefore, the results indicated that the pH and the growth of sundry microbes in complete feed could be significantly reduced and inhibited by fermentation with L. casei and L. plantarum P8. At the same time L. casei and L. plantanun P8 possesses fine stability in feed.%研究了益生乳酸菌干酪乳杆菌Zhang( Lactobacillus casei Zhang)和植物乳杆菌P8(Lactobacillus plantarum P8)对全价饲料pH及微生物类群变化的影响.分别将L.casei Zhang、L.plaruarum P8单一菌种及复合菌种(1∶1)以6.30 lg cfu/g的接种总量发酵全价饲料,测定25℃10d发酵期间全价饲料pH和微生物类群的变化,应用选择培养

  6. Oral immunization of mice against Clostridium perfringens epsilon toxin with a Lactobacillus casei vector vaccine expressing epsilon toxoid.

    Science.gov (United States)

    Alimolaei, Mojtaba; Golchin, Mehdi; Daneshvar, Hamid

    2016-06-01

    Clostridium perfringens type D infects ruminants and causes the enterotoxemia disease by ε-toxin. A mutated ε-toxin gene lacking toxicity was designed, synthesized, and cloned into the pT1NX vector and electroporated into Lactobacillus casei competent cells to yield LC-pT1NX-ε recombinant strain. BALB/c mice, immunized orally with this strain, highly induced mucosal, humoral, and cell-mediated immune responses and developed a protection against 200 MLD/ml of the activated ε-toxin. This study showed that the LC-pT1NX-ε could be a promising vaccine candidate against the enterotoxemia disease. PMID:27012151

  7. Differential expression of cro, the lysogenic cycle repressor determinant of bacteriophage A2, in Lactobacillus casei and Escherichia coli.

    Science.gov (United States)

    Escobedo, Susana; Rodríguez, Isabel; García, Pilar; Suárez, Juan E; Carrasco, Begoña

    2014-04-01

    Expression of bacteriophage A2-encoded cro in Escherichia coli gives rise to two co-linear polypeptides, Cro and Cro*, which were proposed to form a regulatory tandem to modulate the frequency with which the phage would choose between the lytic and the lysogenic cycles. In this communication, it is reported that Cro is the canonical product of the gene cro while Cro* results from a -1 ribosome frameshift during translation and is twelve amino acids shorter than Cro. However, frameshifting was not observed during phage development in Lactobacillus casei. Furthermore, wild type phages and cro-frameshifting negative mutants present the same phenotype, thus corroborating that only the canonical form of Cro is needed to produce a viable phage progeny. PMID:24457071

  8. Fermented milk containing Lactobacillus casei strain Shirota prevents the onset of physical symptoms in medical students under academic examination stress.

    Science.gov (United States)

    Kato-Kataoka, A; Nishida, K; Takada, M; Suda, K; Kawai, M; Shimizu, K; Kushiro, A; Hoshi, R; Watanabe, O; Igarashi, T; Miyazaki, K; Kuwano, Y; Rokutan, K

    2016-01-01

    This pilot study investigated the effects of the probiotic Lactobacillus casei strain Shirota (LcS) on psychological, physiological, and physical stress responses in medical students undertaking an authorised nationwide examination for promotion. In a double-blind, placebo-controlled trial, 24 and 23 healthy medical students consumed a fermented milk containing LcS and a placebo milk, respectively, once a day for 8 weeks until the day before the examination. Psychophysical state, salivary cortisol, faecal serotonin, and plasma L-tryptophan were analysed on 5 different sampling days (8 weeks before, 2 weeks before, 1 day before, immediately after, and 2 weeks after the examination). Physical symptoms were also recorded in a diary by subjects during the intervention period for 8 weeks. In association with a significant elevation of anxiety at 1 day before the examination, salivary cortisol and plasma L-tryptophan levels were significantly increased in only the placebo group (Pstressful situations.

  9. SLA typing using the PCR-SSP method and establishment of the SLA homozygote line in pedigreed SNU miniature pigs.

    Science.gov (United States)

    Yeom, Su-Cheong; Park, Chung-Gyu; Lee, Byeong-Chun; Lee, Wang-Jae

    2010-04-01

    Seoul National University (SNU) miniature pigs represent a closed colony with 24 founder pigs and a well preserved pedigree. Characterization using mRNA sequence analysis was conducted for 6 swine leukocyte antigen (SLA) loci in parental or founder pigs, and 17 defined alleles were detected. Based on these complete coding sequences, 17 sequence specific primers (SSPs) were designed for polymorphic sites. To validate the specificity of each allele SSP, the PCR-SSP was conducted with defined allele clones as templates. PCR-SSP was conducted with the hot start polymerase and touch-down PCR. The parental or found SNU miniature pigs showed overall SLA class I and II heterozygotes. Using the established PCR-SSP method, we conducted SLA typing for breeding stock including 2 pedigreed pigs and identified the novel SLA class II homozygote haplotye (DRA*0201, DRB1*0403, DQA*0102 and DQB1*0701) and 2 SLA homozygote pig lines: SLA class I Hp-3.0 and class II Hp-0.3, and SLA class I Hp-2.0 and class II Hp-0.2. We thought that our PCR-SSP SLA typing method could be applicable for new SLA homozygote line establishment by assignment and scheduled breeding.

  10. The effect of Lactobacillus casei and Bifidobacterium breve on antibiotic-associated diarrhea treatment: randomized double-blind clinical trial

    Directory of Open Access Journals (Sweden)

    Daniela Nogueira Prado de Souza

    2012-02-01

    Full Text Available INTRODUCTION: Antibiotic-associated diarrhea (AAD is an important side effect of this specific class of drugs. The objective of this study was to investigate the effect of the use of probiotics in the treatment of AAD. METHODS: A group of hospitalized patients, who contracted diarrhea during or after 7 days of suspension of antimicrobial medication, was blindly randomized to receive a standardized diet associated with the use of the probiotics (Lactobacillus casei and Bifidobacterium breve or its corresponding placebo, three times a day. RESULTS: Seventy patients were studied. For the experimental (n=35 and control (n=35 groups, respectively, the average time of treatment was 5.06±2.18 and 5.49±3.17 days (p=0.95, and the average duration of diarrhea, among those who were healed, was 4.87±2.13 and 4.52±2.55 days (p=0.36. Four (11.4% patients who received probiotics and ten (28.6% who received the placebo were not cured (p=0.13, and relapse rates were similar between both groups. Seven patients from each group, in addition to diarrhea, presented cases of bloating and/or abdominal cramps and/or vomiting (p=1.00. CONCLUSIONS: In this light, it is concluded that L. casei associated with B. breve, in the administered dosage and frequency, has no effect on the antibiotic-associated diarrhea. Similar studies need to be conducted with higher doses of these or other probiotics.

  11. Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on hepatic steatosis in Zucker rats.

    Directory of Open Access Journals (Sweden)

    Julio Plaza-Diaz

    Full Text Available We have previously described the safety and immunomodulatory effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 in healthy volunteers. The scope of this work was to evaluate the effects of these probiotic strains on the hepatic steatosis of obese rats. We used the Zucker rat as a genetic model of obesity. Zucker-Lepr(fa/fa rats received one of three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNCM I-4035, or a placebo for 30 days. An additional group of Zucker-lean+/fa rats received a placebo for 30 days. No alterations in intestinal histology, in the epithelial, lamina propria, muscular layers of the ileal or colonic mucosa, or the submucosae, were observed in any of the experimental groups. Triacylglycerol content decreased in the liver of Zucker-Lepr(fa/fa rats that were fed L. rhamnosus, B. breve, or the mixture of B. breve and L. paracasei. Likewise, the area corresponding to neutral lipids was significantly smaller in the liver of all four groups of Zucker-Lepr(fa/fa rats that received probiotics than in rats fed the placebo. Zucker-Lepr(fa/fa rats exhibited significantly greater serum LPS levels than Zucker-lean+/fa rats upon administration of placebo for 30 days. In contrast, all four groups of obese Zucker-Lepr(fa/fa rats that received LAB strains exhibited serum LPS concentrations similar to those of Zucker-lean+/fa rats. Serum TNF-α levels decreased in the Zucker-Lepr(fa/fa rats that received B. breve, L. rhamnosus, or the mixture, whereas L. paracasei feeding decreased IL-6 levels in the serum of Zucker-Lepr(fa/fa rats. In conclusion, the probiotic strains reduced hepatic steatosis in part by lowering serum LPS, and had an anti-inflammatory effect in obese Zucker rats.

  12. Associations between the human intestinal microbiota, Lactobacillus rhamnosus GG and serum lipids indicated by integrated analysis of high-throughput profiling data

    Directory of Open Access Journals (Sweden)

    Leo Lahti

    2013-02-01

    Full Text Available Accumulating evidence indicates that the intestinal microbiota regulates our physiology and metabolism. Bacteria marketed as probiotics confer health benefits that may arise from their ability to affect the microbiota. Here high-throughput screening of the intestinal microbiota was carried out and integrated with serum lipidomic profiling data to study the impact of probiotic intervention on the intestinal ecosystem, and to explore the associations between the intestinal bacteria and serum lipids. We performed a comprehensive intestinal microbiota analysis using a phylogenetic microarray before and after Lactobacillus rhamnosus GG intervention. While a specific increase in the L. rhamnosus-related bacteria was observed during the intervention, no other changes in the composition or stability of the microbiota were detected. After the intervention, lactobacilli returned to their initial levels. As previously reported, also the serum lipid profiles remained unaltered during the intervention. Based on a high-resolution microbiota analysis, intake of L. rhamnosus GG did not modify the composition of the intestinal ecosystem in healthy adults, indicating that probiotics confer their health effects by other mechanisms. The most prevailing association between the gut microbiota and lipid profiles was a strong positive correlation between uncultured phylotypes of Ruminococcus gnavus-group and polyunsaturated serum triglycerides of dietary origin. Moreover, a positive correlation was detected between serum cholesterol and Collinsella (Coriobacteriaceae. These associations identified with the spectrometric lipidome profiling were corroborated by enzymatically determined cholesterol and triglyceride levels. Actinomycetaceae correlated negatively with triglycerides of highly unsaturated fatty acids while a set of Proteobacteria showed negative correlation with ether phosphatidylcholines. Our results suggest that several members of the Firmicutes

  13. Investigation of biological activity of polar extracts isolated from Phlomis crinita Cav ssp. mauritanica Munby.

    Science.gov (United States)

    Limem-Ben Amor, Ilef; Skandrani, Ines; Boubaker, Jihed; Ben Sghaïer, Mohamed; Neffati, Aicha; Bhouri, Wissem; Bouhlel, Ines; Chouchane, Nabil; Kilani, Soumaya; Guedon, Emmanuel; Ghoul, Mohamed; Ghedira, Kamel; Chekir-Ghedira, Leila

    2009-01-01

    The lyophilized infusion, the methanol, the ethyl acetate, and the total oligomer flavonoid (TOF)-enriched extracts prepared from the dried leaves of Phlomis crinita Cav. ssp. mauritanica Munby were investigated for the contents of flavonoids, tannins, coumarines and steroids. Antibacterial activity was investigated toward five bacterial strains. An inhibitory effect was observed against Staphyllococcus aureus and Enterococcus feacalis, and the minimal inhibitory concentrations ranged from 2.5 to 5 mg/mL of extract. The tested extracts exhibit an important free radical scavenging activity toward the 1,1-diphenyl 2-picrylhydrazyl (DPPH) free radical; with IC(50) values of 30.5, 6, 32, and 31.5 microg/mL, respectively, in the presence of lyophilized infusion, the TOF, the methanol, and the ethyl acetate extracts. Genotoxic and antigenotoxic properties of the different extracts were studied by using the SOS chromotest with Escherichia coli PQ37. The lyophilized infusion and TOF extracts obtained from P. crinita ssp. mauritanica showed no genotoxicity, whereas methanol and ethyl acetate extracts are considered as marginally genotoxic. On the other hand, we showed that each extract inhibited the mutagenicity induced by aflatoxin B1 (AFB1) (10 microg/assay) and nifuroxazide (NF) (10 microg/assay). The ethyl acetate extract showed the strongest level of protection toward the genotoxicity induced by both directly and indirectly genotoxic NF and AFB1. These tests proved that the lyophilized infusion possesses an antiradical activity likewise, it showed no genotoxic effect; that is why we choose this extract to assess its antiulcerogenic activity by using an ethanol-induced ulcerogenesis model in the rat. This test demonstrates that 300 mg/kg of a P. crinita ssp. mauritanica lyophilized infusion was more effective than the reference compound, cimetidine. PMID:19514937

  14. Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on Hepatic Steatosis in Zucker Rats

    OpenAIRE

    Julio Plaza-Diaz; Carolina Gomez-Llorente; Francisco Abadia-Molina; Maria Jose Saez-Lara; Laura Campaña-Martin; Sergio Muñoz-Quezada; Fernando Romero; Angel Gil; Luis Fontana

    2014-01-01

    We have previously described the safety and immunomodulatory effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 in healthy volunteers. The scope of this work was to evaluate the effects of these probiotic strains on the hepatic steatosis of obese rats. We used the Zucker rat as a genetic model of obesity. Zucker-Lepr(fa/fa) rats received one of three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNC...

  15. Estudo da viabilidade de Bifidobacterium animalis ssp. lactis em suco de Yacon

    OpenAIRE

    Watanabe, Felipe Miguel Farion

    2013-01-01

    Resumo: A maioria dos produtos probióticos são elaborados à base de leite, com desvantagens aos consumidores intolerantes à lactose, tornando assim produtos probióticos não lácteos vantajosos. O yacon é uma planta que acumula em suas raízes compostos prebióticos conhecidos como frutooligossacarídeos. O objetivo deste trabalho foi produzir o suco de yacon e utilizá-lo como matriz não láctea para a bactéria probiótica Bifidobacterium animalis ssp. lactis. O yacon apresentou rápido escurecimento...

  16. PENGEMBANGAN SUBJECT SPECIFIC PEDAGOGY (SSP IPA TERPADU UNTUK MENINGKATKAN HASIL BELAJAR SISWA

    Directory of Open Access Journals (Sweden)

    Fitri Yuliawati

    2016-05-01

    Full Text Available Background of this research is the analysis of the results of interviews some junior secondary schools in Yogyakarta and the conclusion that teachers do not use an integrated science teaching and teachers still find difficulties in the application of learning science in an integrated manner. It can be influenced by several factors, such as the lack of reference used by teachers in presenting the material Integrated Science relevantly, most of science teachers are from educational background of chemistry, physics, and biology instead of science education, so that teachers find difficulties to create an integrated learning of science. In addition, teachers feel difficulty in determining the depth of the material, limits of integration in integrated science teaching, and did not know the concept of integrated science teaching. This research is a development research. Learning tools developed included : student books, lesson plans, student activity sheets and evaluation tools. The development of the learning which is done in this study use the models of 4D development which includes the step of Define (definition which at this stage conducted a needs analysis. Design : it is the stage of Subject Specific Pedagogy (SSP software design. Development, it is the stage of development after the draft was made followed by a learning device validation by experts. This stage is also conducted to seek input from all the responses, reactions and comments from teachers, students, and observers so that it can be used for further improvement of science teaching later. The Disseminate, it is the stage of field tests are widely but not done. Data collection instruments used in this study include : test items and questionnaire. The conclusion of this development research are as follows : the results of the validation SSP integrated science by learning tools expert, material experts and media experts indicate the category of Very Good (SB so that SSP integrated

  17. Antifungal activity of Lactobacillus paracasei ssp. tolerans isolated from a sourdough bread culture.

    Science.gov (United States)

    Hassan, Yousef I; Bullerman, Lloyd B

    2008-01-15

    Lactic acid bacteria were isolated from four different sourdough bread cultures previously investigated for antifungal activity. A total of 116 isolates were obtained and screened for antifungal activity against a battery of molds. The most inhibitory isolate obtained was identified by API 50 CHL and 16s ribosomal RNA genotyping and found to be Lactobacillus paracasei ssp. tolerans. This isolate completely inhibited the growth of Fusarium proliferatum M 5689, M 5991 and Fusarium graminearum R 4053 compared to controls in a dual agar plate assay.

  18. Accumulation of biologically active furanocoumarins in Ruta graveolens ssp. divaricata (Tenore) Gams in vitro culture.

    Science.gov (United States)

    Ekiert, H; Abou-Mandour, A A; Czygan, F Ch

    2005-01-01

    The study was designed to investigate dynamics of accumulation of five linear furanocoumarins and umbelliferone in stationary liquid cultures of Ruta graveolens ssp. divaricata (Tenore) Gams during 6-week growth cycles. The contents of individual metabolites in biomass increased 1.8-3.5 times while their total content rose 2.3 times. Maximum contents of xanthotoxin, bergapten and isopimpinellin (112.3, 76.2 and 84.0mg/100g d.w., respectively) and maximum total content of all metabolites (283.4 mg/100 g d.w.), obtained on 35th culture day, are interesting from practical point of view.

  19. Atrocalopteryx melli orohainani ssp. nov. on the Island of Hainan, China (Zygoptera: Calopterygidae)

    OpenAIRE

    Guan, Z.; Han, B-P; Dumont, Henri

    2012-01-01

    The new sp. is described from the mountain core of Hainan, southern China, where it usually occurs at altitudes not lower than 300 m asl. It lives on the same type of small, shaded rivers as the nominate ssp. on the continent, and is distinguished by its larger size, slightly less enfumed wings, and a 2.6% difference in the sequence of the barcoding portion of the mitochodrial DNA-cytochrome c oxidase subunit I gene (COI). Holotype male: Diaoluoshan mountain, 6-VIII-2011; deposited in the Ins...

  20. Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Gianfranco Grompone

    Full Text Available Numerous studies have shown that resistance to oxidative stress is crucial to stay healthy and to reduce the adverse effects of aging. Accordingly, nutritional interventions using antioxidant food-grade compounds or food products are currently an interesting option to help improve health and quality of life in the elderly. Live lactic acid bacteria (LAB administered in food, such as probiotics, may be good antioxidant candidates. Nevertheless, information about LAB-induced oxidative stress protection is scarce. To identify and characterize new potential antioxidant probiotic strains, we have developed a new functional screening method using the nematode Caenorhabditis elegans as host. C. elegans were fed on different LAB strains (78 in total and nematode viability was assessed after oxidative stress (3 mM and 5 mM H(2O(2. One strain, identified as Lactobacillus rhamnosus CNCM I-3690, protected worms by increasing their viability by 30% and, also, increased average worm lifespan by 20%. Moreover, transcriptomic analysis of C. elegans fed with this strain showed that increased lifespan is correlated with differential expression of the DAF-16/insulin-like pathway, which is highly conserved in humans. This strain also had a clear anti-inflammatory profile when co-cultured with HT-29 cells, stimulated by pro-inflammatory cytokines, and co-culture systems with HT-29 cells and DC in the presence of LPS. Finally, this Lactobacillus strain reduced inflammation in a murine model of colitis. This work suggests that C. elegans is a fast, predictive and convenient screening tool to identify new potential antioxidant probiotic strains for subsequent use in humans.

  1. Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans.

    Science.gov (United States)

    Grompone, Gianfranco; Martorell, Patricia; Llopis, Silvia; González, Núria; Genovés, Salvador; Mulet, Ana Paula; Fernández-Calero, Tamara; Tiscornia, Inés; Bollati-Fogolín, Mariela; Chambaud, Isabelle; Foligné, Benoit; Montserrat, Agustín; Ramón, Daniel

    2012-01-01

    Numerous studies have shown that resistance to oxidative stress is crucial to stay healthy and to reduce the adverse effects of aging. Accordingly, nutritional interventions using antioxidant food-grade compounds or food products are currently an interesting option to help improve health and quality of life in the elderly. Live lactic acid bacteria (LAB) administered in food, such as probiotics, may be good antioxidant candidates. Nevertheless, information about LAB-induced oxidative stress protection is scarce. To identify and characterize new potential antioxidant probiotic strains, we have developed a new functional screening method using the nematode Caenorhabditis elegans as host. C. elegans were fed on different LAB strains (78 in total) and nematode viability was assessed after oxidative stress (3 mM and 5 mM H(2)O(2)). One strain, identified as Lactobacillus rhamnosus CNCM I-3690, protected worms by increasing their viability by 30% and, also, increased average worm lifespan by 20%. Moreover, transcriptomic analysis of C. elegans fed with this strain showed that increased lifespan is correlated with differential expression of the DAF-16/insulin-like pathway, which is highly conserved in humans. This strain also had a clear anti-inflammatory profile when co-cultured with HT-29 cells, stimulated by pro-inflammatory cytokines, and co-culture systems with HT-29 cells and DC in the presence of LPS. Finally, this Lactobacillus strain reduced inflammation in a murine model of colitis. This work suggests that C. elegans is a fast, predictive and convenient screening tool to identify new potential antioxidant probiotic strains for subsequent use in humans. PMID:23300685

  2. Metagenomic analyses of alcohol induced pathogenic alterations in the intestinal microbiome and the effect of Lactobacillus rhamnosus GG treatment.

    Directory of Open Access Journals (Sweden)

    Lara Bull-Otterson

    Full Text Available Enteric dysbiosis plays an essential role in the pathogenesis of alcoholic liver disease (ALD. Detailed characterization of the alterations in the gut microbiome is needed for understanding their pathogenic role in ALD and developing effective therapeutic approaches using probiotic supplementation. Mice were fed liquid Lieber-DeCarli diet without or with alcohol (5% v/v for 6 weeks. A subset of mice were administered the probiotic Lactobacillus rhamnosus GG (LGG from 6 to 8 weeks. Indicators of intestinal permeability, hepatic steatosis, inflammation and injury were evaluated. Metagenomic analysis of the gut microbiome was performed by analyzing the fecal DNA by amplification of the V3-V5 regions of the 16S rRNA gene and large-scale parallel pyrosequencing on the 454 FLX Titanium platform. Chronic ethanol feeding caused a decline in the abundance of both Bacteriodetes and Firmicutes phyla, with a proportional increase in the gram negative Proteobacteria and gram positive Actinobacteria phyla; the bacterial genera that showed the biggest expansion were the gram negative alkaline tolerant Alcaligenes and gram positive Corynebacterium. Commensurate with the qualitative and quantitative alterations in the microbiome, ethanol caused an increase in plasma endotoxin, fecal pH, hepatic inflammation and injury. Notably, the ethanol-induced pathogenic changes in the microbiome and the liver were prevented by LGG supplementation. Overall, significant alterations in the gut microbiome over time occur in response to chronic alcohol exposure and correspond to increases in intestinal barrier dysfunction and development of ALD. Moreover, the altered bacterial communities of the gut may serve as significant therapeutic target for the prevention/treatment of chronic alcohol intake induced intestinal barrier dysfunction and liver disease.

  3. SURVIVING OF COMMERCIAL PROBIOTIC STRAIN Lactobacillus rhamnosus GG IN SLOVAK COW LUMP CHEESE EXPERIMENTALLY INOCULATED WITH Listeria innocua

    Directory of Open Access Journals (Sweden)

    Andrea Lauková

    2014-08-01

    Full Text Available Cow lump cheese represents a traditional Slovak cheese. It belongs to fresh types of cheeses. The aim of this study was to test surviving of commercial probiotic strain Lactobacillus rhamnosus GG in cow lump cheese experimentally infected with L. innocua; (listeriae are contaminants and to check the suitability of GG strain as additive for this product. The counts of GG strain in cow lump cheeses were well balanced during whole experiment. It was found in the counts from 5.48 ± 0.15 to 7.77 ±1.50 log10 cfu/g. Its maximum in cheese was 7.77 ± 1.30 log10 cfu/g on day 7 with stability up to day 14. The identity of GG strain isolated from cheeses was confirmed by PCR. The counts of other lactic acid bacteria were also well balanced during the whole experiment in the experimental cheeses with stability up to day 14. Only in E1/GG cheese, the highest number of LAB was detected (10.60 ±1.26 log10 cfu/g. The count of L. innocua LMG 13568 was not influenced. The pH and lactic acid values were not negatively influenced. Visually, the GG cheese provided a good structure (consistency.It can be disputed that shelf-life of the product could be maintained by this way and/or the product itself with GG strain can be consumed as afunctional food or to serve as a probiotic strain carrier.

  4. An L-Fucose Operon in the Probiotic Lactobacillus rhamnosus GG Is Involved in Adaptation to Gastrointestinal Conditions.

    Science.gov (United States)

    Becerra, Jimmy E; Yebra, María J; Monedero, Vicente

    2015-06-01

    L-Fucose is a sugar present in human secretions as part of human milk oligosaccharides, mucins, and other glycoconjugates in the intestinal epithelium. The genome of the probiotic Lactobacillus rhamnosus GG (LGG) carries a gene cluster encoding a putative L-fucose permease (fucP), L-fucose catabolic pathway (fucI, fucK, fucU, and fucA), and a transcriptional regulator (fucR). The metabolism of L-fucose in LGG results in 1,2-propanediol production, and their fucI and fucP mutants displayed a severe and mild growth defect on L-fucose, respectively. Transcriptional analysis revealed that the fuc genes are induced by L-fucose and subject to a strong carbon catabolite repression effect. This induction was triggered by FucR, which acted as a transcriptional activator necessary for growth on L-fucose. LGG utilized fucosyl-α1,3-N-acetylglucosamine and contrarily to other lactobacilli, the presence of fuc genes allowed this strain to use the L-fucose moiety. In fucI and fucR mutants, but not in fucP mutant, L-fucose was not metabolized and it was excreted to the medium during growth on fucosyl-α1,3-N-acetylglucosamine. The fuc genes were induced by this fucosyl-disaccharide in the wild type and the fucP mutant but not in a fucI mutant, showing that FucP does not participate in the regulation of fuc genes and that L-fucose metabolism is needed for FucR activation. The l-fucose operon characterized here constitutes a new example of the many factors found in LGG that allow this strain to adapt to the gastrointestinal conditions. PMID:25819967

  5. Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG.

    Science.gov (United States)

    Orlando, Antonella; Linsalata, Michele; Russo, Francesco

    2016-06-01

    Vitamin K (VK), an essential nutrient associated with the clotting cascade, has also been demonstrated to have anticancer properties in various cancer cells including colon cancer cells. Also probiotics have gained interest as potential anticancer agents. Among them, Lactobacillus rhamnosus GG (L.GG) has been shown to inhibit cell proliferation and polyamine biosynthesis as well as to induce apoptosis in different human gastrointestinal cancer cells. Nevertheless, the exact mechanisms involved in these actions are not completely elucidated. Therefore, the aims of the present study were to evaluate in three differently graded human colon cancer cells (namely Caco-2, HT-29 and SW480) the effects of increasing VK1 concentrations, administered alone or in combination with viable L.GG, on the cell proliferation evaluated by MTT test, apoptosis investigated by Bax/Bcl-2 ratio and the percentage of the apoptotic cells, and the cell cycle evaluated by MUSE cell analyzer. Both VK1 and L.GG administered alone up to 72 h, caused inhibition of proliferation, induction of apoptosis and the cell cycle arrest in all the tested colon cancer cells. When VK1 and L.GG were co-administered, the addition of increasing VK1 concentrations potentiated the probiotic antiproliferative effect in a dose-dependent manner, being also related to the individual features of each cell line. The effect was more evident in Caco-2 and HT-29 cells compared to the less differentiated SW480. The enhanced antiproliferative efficacy due to co-administration of L.GG and VK1 could represent a suitable option in a functional food strategy for cancer growth inhibition and chemoprevention. PMID:27035094

  6. An L-Fucose Operon in the Probiotic Lactobacillus rhamnosus GG Is Involved in Adaptation to Gastrointestinal Conditions.

    Science.gov (United States)

    Becerra, Jimmy E; Yebra, María J; Monedero, Vicente

    2015-06-01

    L-Fucose is a sugar present in human secretions as part of human milk oligosaccharides, mucins, and other glycoconjugates in the intestinal epithelium. The genome of the probiotic Lactobacillus rhamnosus GG (LGG) carries a gene cluster encoding a putative L-fucose permease (fucP), L-fucose catabolic pathway (fucI, fucK, fucU, and fucA), and a transcriptional regulator (fucR). The metabolism of L-fucose in LGG results in 1,2-propanediol production, and their fucI and fucP mutants displayed a severe and mild growth defect on L-fucose, respectively. Transcriptional analysis revealed that the fuc genes are induced by L-fucose and subject to a strong carbon catabolite repression effect. This induction was triggered by FucR, which acted as a transcriptional activator necessary for growth on L-fucose. LGG utilized fucosyl-α1,3-N-acetylglucosamine and contrarily to other lactobacilli, the presence of fuc genes allowed this strain to use the L-fucose moiety. In fucI and fucR mutants, but not in fucP mutant, L-fucose was not metabolized and it was excreted to the medium during growth on fucosyl-α1,3-N-acetylglucosamine. The fuc genes were induced by this fucosyl-disaccharide in the wild type and the fucP mutant but not in a fucI mutant, showing that FucP does not participate in the regulation of fuc genes and that L-fucose metabolism is needed for FucR activation. The l-fucose operon characterized here constitutes a new example of the many factors found in LGG that allow this strain to adapt to the gastrointestinal conditions.

  7. Modulation of respiratory TLR3-antiviral response by probiotic microorganisms: lessons learned from Lactobacillus rhamnosus CRL1505

    Directory of Open Access Journals (Sweden)

    Haruki eKitazawa

    2014-05-01

    Full Text Available Respiratory syncytial virus (RSV is the leading cause of lower respiratory tract illness in infants and young children. Host immune response is implicated in both protective and immunopathological mechanisms during RSV infection. Activation of Toll-like receptor (TLR-3 in innate immune cells by RSV can induce airway inflammation, protective immune response, and pulmonary immunopathology. Understanding the interaction between RSV and its host is crucial for the development of novel therapeutic strategies as well as safe and effective vaccines. Several studies have centered on whether probiotic microorganisms with the capacity to stimulate the immune system (immunobiotics might sufficiently stimulate the common mucosal immune system to provide protection in the respiratory tract. In this regard, it was demonstrated that some orally administered immunobiotics do have the ability to stimulate respiratory immunity and increase resistance to viral infections. Moreover, during the last decade scientists have significantly advanced in the knowledge of the cellular and molecular mechanisms involved in the protective effect of immunobiotics in the respiratory tract. This review examines the current scientific literature dealing with the use of immunobiotic strains to prevent viral respiratory infections. More specifically, the article discuss the mechanisms involved in the capacity of the immunobiotic strain Lactobacillus rhamnosus CRL1505 to beneficially modulate the immune response triggered by TLR3 activation in the respiratory tract and to increase the resistance to RSV infection in infant mice. In addition, we review the role of interferon (IFN-γ and interleukin (IL-10 in the immunoregulatory effect of the CRL1505 strain that has been successfully used for reducing incidence and morbidity of viral airways infections in children.

  8. ENZYMATIC ACTIVITY AND ANTIBIOTIC RESISTANCE PROFILE OF LACTOBACILLUS PARACASEI SSP. PARACASEI-1 ISOLATED FROM REGIONAL YOGURTS OF BANGLADESH

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    Ummay Honi

    2013-12-01

    Full Text Available Lactobacillus paracasei ssp. paracasei-1 was identified from traditional yogurts of Khulna region, Bangladesh and its enzyme and antibiotic resistance profiles were determined. A commercially available API Zym kit was employed to determine the activities of 19 different enzymes. We found that L. paracasei ssp. paracasei-1 showed strong activities for several enzymes, viz. leucine arylamidase, valine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, α –Glucosidase, N-Acetyl- β- glucosaminidase while activities for other enzymes were absent. Antibiotic resistance profile was assessed by minimum inhibitory concentration (MIC test for 61 major antibiotics and 4 antifungal agents obtained from commercial sources in MRS Agar media. The strain generally showed resistance to gram negative spectrum antibiotic while it showed susceptibility towards β-lactam antibiotic to gram positive spectrum antibiotic. The findings provide the therapeutic basis of using L. paracasei ssp. paracasei-1 in finished food products.

  9. Étude de l'effet antibactérien des huiles essentielles de Thymus ciliatus ssp coloratus

    OpenAIRE

    HADDOUCHE, Khalida

    2015-01-01

    Etude de l'effet antibactérien de l'huile essentielle de Thymus ciliatus ssp coloratus Résumé Les plantes médicinales constituent une source immense de métabolites secondaires, dont les huiles essentielles dotées de nombreuses activités biologiques ; souvent recherchées dans le domaine phytopharmaceutique et en agro-alimentaire. Le présent travail porte sur une étude qualitative du pouvoir antibactérien de l'huile essentielle de la plante médicinale: Thymus ciliatus ssp c...

  10. The first reported catheter-related Brevibacterium casei bloodstream infection in a child with acute leukemia and review of the literature.

    Science.gov (United States)

    Bal, Zumrut Sahbudak; Sen, Semra; Karapinar, Deniz Yilmaz; Aydemir, Sohret; Vardar, Fadil

    2015-01-01

    Brevibacterium spp. are catalase-positive, non-spore-forming, non motile, aerobic Gram-positive rods that were considered apathogenic until a few reports of infections in immunocompromised patients had been published. To the best of our knowledge, this is the first report of B. casei catheter-related bloodstream infection in a child with acute leukemia. We aim to enhance the awareness of pediatric hematology and infectious disease specialists about this pathogen and review of the literature. PMID:25636191

  11. Milk digesta and milk protein fractions influence the adherence of Lactobacillus gasseri R and Lactobacillus casei FMP to human cultured cells.

    Science.gov (United States)

    Volstatova, Tereza; Havlik, Jaroslav; Potuckova, Miroslava; Geigerova, Martina

    2016-08-10

    Adhesion to the intestinal epithelium is considered an important feature of probiotic bacteria, which may increase their persistence in the intestine, allowing them to exert their beneficial health effect or promote the colonisation process. However, this feature might be largely dependent on the host specificity or diet. In the present study, we investigated the effect of selected milks and milk protein fractions on the ability of selected lactobacilli to adhere to the cells of an intestinal model based on co-culture Caco-2/HT29-MTX cell lines. Most milk digesta did not significantly affect bacterial adhesion except for UHT-treated milk and sheep milk. The presence of UHT-treated milk digesta reduced the adhesion of Lactobacillus gasseri R by 61% but not that of Lactobacillus casei FMP. However, sheep milk significantly increased the adherence of L. casei FMP (P < 0.05) but not of L. gasseri R. Among the protein fractions, rennet casein (RCN) and bovine serum albumin (BSA) showed reproducible patterns and strain-specific effects on bacterial adherence. While RCN reduced the adherence of L. gasseri R to <50% compared to the control, it did not have a significant effect on L. casei FMP. In contrast, BSA reduced L. casei FMP adherence to a higher extent than that of L. gasseri R. Whey protein (WH) tended to increase the adherence of both strains by 130%-180%. Recently, interactions between the host diet and its microbiota have attracted considerable interest. Our results may explain one of the aspects of the role of milk in the development of microbiota or support of probiotic supplements. Based on our data, we conclude that the persistence of probiotic strains supplemented as part of dairy food or constitutional microbiota in the gut might be affected negatively or positively by the food matrix through complex strain or concentration dependent effects.

  12. Characterization of the cysK2-ctl1-cysE2 gene cluster involved in sulfur metabolism in Lactobacillus casei.

    Science.gov (United States)

    Bogicevic, Biljana; Irmler, Stefan; Portmann, Reto; Meile, Leo; Berthoud, Hélène

    2012-01-16

    The up- and downstream regions of ctl1 and ctl2 that encode a cystathionine lyase were analyzed in various Lactobacillus casei strains. ctl1 and ctl2 were found to be part of a gene cluster encoding two other open reading frames. One of the two open reading frames precedes ctl1 and encodes a putative cysteine synthase. The other open reading frame lies downstream of ctl1 and encodes a putative serine acetyltransferase. The gene cluster is not present in the publicly available genome sequences of L. casei ATCC 334, BL23 and Zhang. Apparently, the gene cluster was acquired by a horizontal gene transfer event and can also be found in other lactic acid bacteria such as Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. RT-PCR was used to analyze the expression of the gene cluster. Additionally, an mass spectrometry-based selected reaction monitoring method was developed for quantifying Ctl1 in a cell-free extract of lactic acid bacteria. The gene cluster cysK2-ctl1-cysE2 was expressed as single transcript, and expression was down-regulated by cysteine. In addition, cystathionine lyase activity present in cell-free extracts disappeared when L. casei was grown in the presence of cysteine. Whereas the transcript and the gene product of ctl1 protein were found in all studied ctl1(+)L. casei strains, only the transcript but not the protein or cystathionine lyase activity was detected in L. helveticus FAM2888, L. delbrueckii subsp. bulgaricus ATCC 11842 and S. thermophilus FAM17014, which actually possess a homolog of the cysK2-ctl1-cysE2 gene cluster.

  13. Optimizing conditions for the growth of Lactobacillus casei YIT 9018 in tryptone-yeast extract-glucose medium by using response surface methodology.

    OpenAIRE

    Oh, S.; Rheem, S; Sim, J.; Kim, S.; Baek, Y.

    1995-01-01

    This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design for allocation of treatment combinations. A second-order polynomial regression model, which was used at first for analysis of the experiment, had a significant lack of fit. There...

  14. Lactoferrin affects the adherence and invasion of Streptococcus dysgalactiae ssp. dysgalactiae in mammary epithelial cells.

    Science.gov (United States)

    O'Halloran, Fiona; Beecher, Christine; Chaurin, Valerie; Sweeney, Torres; Giblin, Linda

    2016-06-01

    Streptococcus dysgalactiae ssp. dysgalactiae is an important causative agent of bovine mastitis worldwide. Lactoferrin is an innate immune protein that is associated with many functions including immunomodulatory, antiproliferative, and antimicrobial properties. This study aimed to investigate the interactions between lactoferrin and a clinical bovine mastitis isolate, Strep. dysgalactiae ssp. dysgalactiae DPC5345. Initially a deliberate in vivo bovine intramammary challenge was performed with Strep. dysgalactiae DPC5345. Results demonstrated a significant difference in lactoferrin mRNA levels in milk cells between the control and infused quarters 7h postinfusion. Milk lactoferrin levels in the Strep. dysgalactiae DPC5345 infused quarters were significantly increased compared with control quarters at 48h postinfusion. In vitro studies demonstrated that lactoferrin had a bacteriostatic effect on the growth of Strep. dysgalactiae DPC5345 and significantly decreased the ability of the bacteria to internalize into HC-11 mammary epithelial cells. Confocal microscopy images of HC-11 cells exposed to Strep. dysgalactiae and lactoferrin further supported this effect by demonstrating reduced invasion of bacteria to HC-11 cells. The combined data suggest that a bovine immune response to Strep. dysgalactiae infection includes a significant increase in lactoferrin expression in vivo, and based on in vitro data, lactoferrin limits mammary cell invasion of this pathogen by binding to the bacteria and preventing its adherence. PMID:27016824

  15. The Huygens surface science package (SSP): Flight performance review and lessons learned

    Science.gov (United States)

    Leese, M. R.; Lorenz, R. D.; Hathi, B.; Zarnecki, J. C.

    2012-09-01

    The Surface Science Package (SSP) was one of six instruments flown onboard the Huygens probe to Titan, the largest moon of Saturn, in the framework of the NASA/ESA/ASI Cassini-Huygens mission (Matson et al., 2002). The SSP operated throughout the probe's descent and after landing on Titan on 14th January 2005. This paper reviews scientific results from the Surface Science Package, and also reports previously unpublished flight data which illustrate the performance of the measurement systems in the Titan environment. This review provides some lessons learned that may be useful for further detailed analysis of the Huygens mission data, and for payloads for future missions to Titan, in which there has been recent interest (e.g., the Titan Saturn System Mission (TSSM) (Joint TSSM Science Definition Team, 2009), TANDEM (Coustenis et al., 2009) or the Titan Mare Explorer (TiME) Discovery-class proposal (Stofan et al., 2010)), as well as for planetary probe missions more generally.

  16. Screening sourdough samples for gliadin-degrading activity revealed Lactobacillus casei strains able to individually metabolize the coeliac-disease-related 33-mer peptide.

    Science.gov (United States)

    Alvarez-Sieiro, Patricia; Redruello, Begoña; Ladero, Victor; Martín, Maria Cruz; Fernández, María; Alvarez, Miguel A

    2016-05-01

    A selective culture medium containing acid-hydrolyzed gliadins as the sole nitrogen source was used in the search for sourdough-indigenous lactic acid bacteria (LAB) with gliadin-metabolizing activity. Twenty gliadin-degrading LAB strains were isolated from 10 sourdoughs made in different ways and from different geographical regions. Fifteen of the 20 isolated strains were identified as Lactobacillus casei, a species usually reported as subdominant in sourdough populations. The other 5 gliadin-degrading strains belonged to the more commonly encountered sourdough species Leuconostoc mesenteroides and Lactobacillus plantarum. All these strains were shown to be safe in terms of their resistance to antimicrobial agents. When individually incubated with the α2-gliadin-derived immunotoxic 33-mer peptide (97.5 ppm), half of the L. casei strains metabolized at least 50% of it within 24 h. One strain metabolized 82% of the 33-mer peptide within 8 h and made it fully disappear within 12 h. These results reveal for the first time the presence in sourdough of proteolytic L. casei strains with the capacity to individually metabolize the coeliac-disease-related 33-mer peptide. PMID:27021684

  17. The Salmonella type III effector SspH2 specifically exploits the NLR co-chaperone activity of SGT1 to subvert immunity.

    Directory of Open Access Journals (Sweden)

    Amit P Bhavsar

    Full Text Available To further its pathogenesis, S. Typhimurium delivers effector proteins into host cells, including the novel E3 ubiquitin ligase (NEL effector SspH2. Using model systems in a cross-kingdom approach we gained further insight into the molecular function of this effector. Here, we show that SspH2 modulates innate immunity in both mammalian and plant cells. In mammalian cell culture, SspH2 significantly enhanced Nod1-mediated IL-8 secretion when transiently expressed or bacterially delivered. In addition, SspH2 also enhanced an Rx-dependent hypersensitive response in planta. In both of these nucleotide-binding leucine rich repeat receptor (NLR model systems, SspH2-mediated phenotypes required its catalytic E3 ubiquitin ligase activity and interaction with the conserved host protein SGT1. SGT1 has an essential cell cycle function and an additional function as an NLR co-chaperone in animal and plant cells. Interaction between SspH2 and SGT1 was restricted to SGT1 proteins that have NLR co-chaperone function and accordingly, SspH2 did not affect SGT1 cell cycle functions. Mechanistic studies revealed that SspH2 interacted with, and ubiquitinated Nod1 and could induce Nod1 activity in an agonist-independent manner if catalytically active. Interestingly, SspH2 in vitro ubiquitination activity and protein stability were enhanced by SGT1. Overall, this work adds to our understanding of the sophisticated mechanisms used by bacterial effectors to co-opt host pathways by demonstrating that SspH2 can subvert immune responses by selectively exploiting the functions of a conserved host co-chaperone.

  18. A Lactobacillus rhamnosus strain induces protection in different sites after Salmonella enterica subsp. enterica serovar Typhimurium challenge in gnotobiotic and conventional mice

    Directory of Open Access Journals (Sweden)

    A.H. Mendonça

    2014-04-01

    Full Text Available The ability of a Lactobacillus rhamnosus strain isolated from a healthy breast-fed human newborn to reduce the pathological consequences for the host due to an experimental oral infection with Salmonella enterica subsp. enterica serov. Typhimurium in vivo was determined using gnotobiotic and conventional mice. Conventional mice received 0.1mL probiotic milk (8.0 log colony-forming unit daily for 10 days before the oral pathogenic challenge (5.0 log colony-forming unit. Then probiotic treatment was continued until the end of the experiment. Probiotic treatment in germ-free mice consisted of a single dose of the probiotic milk at the beginning of the experiment and a challenge with S. Typhimurium 10 days later (3.0 log colony-forming unit. A protective effect was observed in both gnotobiotic and conventional animals in terms of histopathologic and morphometric data, but in different anatomical sites. This protection was observed in liver and intestines, respectively, for gnotobiotic and conventional mice. However, S. Typhimurium populations were similar in the feces of both treated and control gnotobiotic mice. We conclude that a protective effect of L. rhamnosus against experimental S. Typhimurium was observed. This protection was not due to the reduction of the population of pathogenic bacteria in the intestine.

  19. The antimicrobial properties of the lowbush blueberry (Vaccinium angustifolium) fractional components against foodborne pathogens and the conservation of probiotic Lactobacillus rhamnosus.

    Science.gov (United States)

    Lacombe, Alison; Wu, Vivian C H; White, Jennifer; Tadepalli, Shravaini; Andre, Enroe E

    2012-05-01

    The antimicrobial properties of lowbush blueberry (Vaccinium angustifolium) were studied against Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Lactobacillus rhamnosus to determine which fractional components have antimicrobial effects and which microorganisms are most susceptible to these antimicrobial properties. Lowbush blueberry extract (F1) was separated using a C-18 Sep-Pak cartridge into monomeric phenolics (F2) and anthocyanins plus proanthocyanidins (F3). Fraction 3 was further separated into anthocyanins (F4) and proanthocyanidins (F5) using a LH-20 Sephadex column. Each fraction was initially screened for antimicrobial properties using agar diffusion assay. Treatments that demonstrated inhibition were further analyzed for inhibition in liquid culture. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using a two-fold dilution series and viable cell counts taken at 0 and 24 h to examine growth reduction. Fraction 3 demonstrated the lowest MICs/MBCs followed by F1, F2, F4, and F5. L. monocytogenes was the most susceptible to blueberry fraction treatment, followed by E. coli O157:H7, and S. Typhimurium. L. rhamnosus was the least susceptible to each fraction treatment. The results can be applied to the field of preventive medicine, food safety, and enrich the understanding of the health benefits of lowbush blueberries. PMID:22265292

  20. Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major.

    Science.gov (United States)

    Dawood, Mahmoud A O; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro; El Basuini, Mohammed F; Hossain, Md Sakhawat; Nhu, Truong H; Dossou, Serge; Moss, Amina S

    2016-02-01

    Pagrus major fingerlings (3·29 ± 0·02 g) were fed with basal diet (control) supplemented with Lactobacillus rhamnosus (LR), Lactococcus lactis (LL), and L. rhamnosus + L. lactis (LR + LL) at 10(6) cell g(-1) feed for 56 days. Feeding a mixture of LR and LL significantly increased feed utilization (FER and PER), intestine lactic acid bacteria (LAB) count, plasma total protein, alternative complement pathway (ACP), peroxidase, and mucus secretion compared with the other groups (P growth performance (Fn wt, WG, and SGR) and protein digestibility than the groups fed an individual LR or the control diet. Superoxide dismutase (SOD) significantly increased in LR and LR + LL groups when compared with the other groups. Moreover, the fish fed LR or LL had better improvement (P growth, feed utilization, body protein and lipid contents, digestibility coefficients (dry matter, protein, and lipid), protease activity, total intestine and LAB counts, hematocrit, total plasma protein, biological antioxidant potential, ACP, serum and mucus LZY and bactericidal activities, peroxidase, SOD, and mucus secretion than the control group. Interestingly, fish fed diets with LR + LL showed significantly lower total cholesterol and triglycerides when compared with the other groups (P < 0.05). These data strongly suggest that a mixture of LR and LL probiotics may serve as a healthy immunostimulating feed additive in red sea bream aquaculture.

  1. The effects of Probiotic on Human Intestinal Microbiota——Taking Lactobacillus casei Zhang as Example%益生菌对肠道菌群的影响——以Lactobacillus casei Zhang研究为例

    Institute of Scientific and Technical Information of China (English)

    张家超; 郭壮; 孙志宏; 陈卫; 张和平

    2011-01-01

    人体肠道内栖居着数以万亿计的微生物,这些微生物在繁衍的过程中逐渐达到种类和数量的平衡,而这种平衡与机体正常代谢密切相关.一旦肠道菌群结构发生失调,各种代谢类疾病便随之而来.益生菌在肠道内的大量繁衍,可调节紊乱的肠道菌群结构并提高机体的免疫能力,进而帮助恢复健康水平.近年来,国际上科学研究的热点逐渐集中于益生菌对肠道菌群的影响以及肠道菌群和疾病的关系.本文概述国内外有关益生菌影响肠道菌群的研究现状.以1株具有完全自主知识产权并且已成功实施产业化应用的益生菌菌株——Lactobacillus casei Zhang为例,详实说明益生菌对人肠道菌群的影响.在此基础上提出本学科现存的若干研究瓶颈及今后的发展方向.%The human gastrointestinal (CI) tract contains an immense number of microorganisms, collectively known as the microbiota. The gut microbiota played important roles in keeping the body health. The colonization of probiotic in human gut stimulated intestinal epithelium renewal and directed the maturation of the immune system. Recent years, more and more researches were focused on the effect of probiotic on human intestinal microbiota and the relationship of gut microflora and disease. This paper makes a summary of the progress of the effect of probiotic on human intestinal microflora. To better understand this subject, we took our research results of the effects of probiotic Lactobacillus casei Zhang on human intestinal microbiota as example.

  2. Influence of milk fermented with Lactobacillus rhamnosus NCDC 17 alone and in combination with herbal ingredients on diet induced adiposity and related gene expression in C57BL/6J mice.

    Science.gov (United States)

    Pothuraju, Ramesh; Sharma, Raj Kumar; Chagalamarri, Jayasimha; Kavadi, Praveen Kumar; Jangra, Surender

    2015-11-01

    Obesity has become a major health problem in developed countries and is rapidly catching up in the developing world due to changes in their life style. Dietary incorporation of functional foods, including probiotic fermented milk and herbal ingredients, is being tried to ameliorate metabolic disorders. In the present study, the effect of dietary supplementation of a probiotic (Lactobacillus rhamnosus NCDC 17) fermented milk alone or either of the herbal preparations (Aloe vera/Gymnema sylvestre powders, 1% w/w) on the progression of obesity has been studied in C57BL/6J mice fed with a high fat diet for 12 weeks. At the end of the experimental period, oral administration of L. rhamnosus and herbs resulted in a significant decrease in the body weight, epididymal fat mass, fasting blood glucose and serum insulin levels. Supplementation of the probiotic L. rhamnosus alone and in combination with herbs showed a significant decrease in the adipocyte cell size and an increase in the number. Finally, obesity related adipokines levels were maintained at normal by the treatment groups. Thus, dietary intervention of milk fermented with probiotic L. rhamnosus alone or in combination with any of the herbal preparations seems to show anti-obesity and anti-inflammatory properties. PMID:26327356

  3. Molecular Characterization of Two Silenced y-type Genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum

    Institute of Scientific and Technical Information of China (English)

    Zhong-Wei Yuan; Qi-Jiao Chen; Lian-Quan Zhang; Ze-Hong Yan; You-Liang Zheng; Deng-Cai Liu

    2009-01-01

    The high molecular weight glutenln subunits (HMW-GSs) are a major class of common wheat storage proteins. The bread-making quality of common wheat flour is influenced by the composition of HMW-GSs. In the present study, two unexpressed 1By genes from Triticum aesitvum L.ssp.yunnanese AS332 and T. aesitvum sep.tibetanum AS908 were respectively cloned and characterized. The results indicated that both of the silenced 1By genes in AS332 and AS908 were 1By9. in contrast to previously reported mechanisms for silenced genes 1Ax and 1Ay, which was due to the insertion of transposon elements or the presence of premature stop codon via base substitution of C→T transition in tdnucleotides CAA or CAG, the silence of 1By9 genes was caused by premature stop codons via the deletion of base A in tdnucleotide CAA, which lead to frameshift mutation and indirectly produced several premature stop codons (TAG) downstream of the coding sequence.

  4. Dietary Lactobacillus rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged by Porcine Rotavirus

    Science.gov (United States)

    Mao, Xiangbing; Gu, Changsong; Hu, Haiyan; Tang, Jun; Chen, Daiwen; Yu, Bing; He, Jun; Yu, Jie; Luo, Junqiu; Tian, Gang

    2016-01-01

    Lactobacillus rhamnosus GG (LGG) has been regarded as a safe probiotic strain. The aim of this study was to investigate whether dietary LGG supplementation could alleviate diarrhea via improving jejunal mucosal barrier function in the weaned piglets challenged by RV, and further analyze the potential roles for apoptosis of jejunal mucosal cells and intestinal microbiota. A total of 24 crossbred barrows weaned at 21 d of age were assigned randomly to 1 of 2 diets: the basal diet and LGG supplementing diet. On day 11, all pigs were orally infused RV or the sterile essential medium. RV infusion increased the diarrhea rate, increased the RV-Ab, NSP4 and IL-2 concentrations and the Bax mRNA levels of jejunal mucosa (P<0.05), decreased the villus height, villus height: crypt depth, the sIgA, IL-4 and mucin 1 concentrations and the ZO-1, occludin and Bcl-2 mRNA levels of jejunal mucosa (P<0.05), and affected the microbiota of ileum and cecum (P<0.05) in the weaned pigs. Dietary LGG supplementation increased the villus height and villus height: crypt depth, the sIgA, IL-4, mucin 1 and mucin 2 concentrations, and the ZO-1, occludin and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05) reduced the Bax mRNA levels of the jejunal mucosa (P<0.05) in weaned pigs. Furthermore, dietary LGG supplementation alleviated the increase of diarrhea rate in the weaned pigs challenged by RV (P<0.05), and relieve the effect of RV infection on the villus height, crypt depth and the villus height: crypt depth of the jejunal mucosa (P<0.05), the NSP4, sIgA, IL-2, IL-4, mucin 1 and mucin 2 concentrations of jejunal mucosa (P<0.05), the ZO-1, occludin, Bax and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05), and the microbiota of ileum and cecum (P<0.05) in the weaned pigs challenged by RV. These results suggest that supplementing LGG in diets alleviated the diarrhea of weaned piglets challenged by RV via inhibiting the virus multiplication and improving the jejunal mucosal barrier function

  5. Hypoallergenic formula with Lactobacillus rhamnosus GGfor babies with colic: A pilot study of recruitment, retention,and fecal biomarkers

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    AIM To investigate recruitment, retention, and estimatesfor effects of formula supplementation withLactobacillus rhamnosus GG (LGG) on inflammatorybiomarkers and fecal microbial community in infants withcolic.METHODS: A prospective, double-blind, placebocontrolledtrial was conducted in otherwise healthyinfants with colic. We screened 74 infants and randomizedand analyzed results in 20 infants [9 receivingLGG (LGG+) and 11 not receiving LGG (LGG-)]. LGG wasincorporated in the formula (Nutramigen?) (minimum of3 × 107 CFU/d) in the LGG+ group. Fecal microbiota andinflammatory biomarkers, including fecal calprotectin(FC), plasma cytokines, circulating regulatory T cells(Tregs), and crying + fussing time were analyzed todetermine optimal time points and effect sizes for alarger trial.RESULTS: Recruitment in this population was slow, withabout 66% of eligible infants willing to enroll; subjectretention was better (75%). These rates were influencedby parents' reluctance to volunteer their infant for aclinical trial and by their tendency to change formulas.The maximal difference of crying + fussing time wasobserved at day 14, comparing the 2 groups, with amean difference of -91 (95%CI: -76, 259) min (P = NS).FC showed no significant difference, but the optimaltime to determine a potential effect was at day 90 [witha mean difference of 121 (95%CI: -48, 291) μg/g stool],observing a lower level of FC in the LGG+ group. Thefecal microbial communities were chaotic, as determinedby Shannon's diversity index and not apparently influencedby the probiotic. No significant change wasobserved in plasma inflammatory cytokines or Tregs,comparing LGG+ to LGG- groups.CONCLUSION: Designing future colic trials involving aprobiotic-supplemented formula for infants in the UnitedStates will require consideration for difficult enrollment.Infants with colic have major variations in feal microbiotaand calprotectin, both of

  6. Analysis of expressed sequence tags from Musa acuminata ssp. burmannicoides, var. Calcutta 4 (AA) leaves submitted to temperature stresses

    NARCIS (Netherlands)

    Santos, C.R.; Martins, N.F.; Horberg, H.M.; Almeida, E.R.P.; Coelho, M.C.F.; Togawa, R.; Silva, F.R.; Caetano, A.R.; Miller, R.N.G.; Souza, M.T.

    2005-01-01

    In order to discover genes expressed in leaves of Musa acuminata ssp. burmannicoides var. Calcutta 4 (AA), from plants submitted to temperature stress, we produced and characterized two full-length enriched cDNA libraries. Total RNA from plants subjected to temperatures ranging from 5°C to 25°C and

  7. Growth Responses of Plantago major L. ssp. pleiosperma (Pilger) to Changes in Mineral Supply : Evidence for Regulation by Cytokinins.

    Science.gov (United States)

    Kuiper, D

    1988-07-01

    Plants of an inbred line of Plantago major ssp. pleiosperma were subjected to an alteration in mineral supply. Observed responses of growth rate and shoot to root ratio are thought to be induced by changes in endogenous cytokinin concentration and not by mineral concentration in plant tissue.

  8. Poplar root exudates contain compounds that induce the expression of MiSSP7 in Laccaria bicolor.

    Science.gov (United States)

    Plett, Jonathan M; Martin, Francis

    2012-01-01

    Communication between organisms is crucial for their survival, especially for sessile organisms such as plants that depend upon interactions with mutualistic organisms to maximize their nutrient acquisition. This communication can take the form of the exchange of volatile compounds, metabolites or effectors - small protein signals secreted from the colonizing cell that change the biology of the host cell.  We recently characterized the first mutualistic effector protein from an ectomycorrhizal fungus, a small secreted protein named MiSSP7 encoded by Laccaria bicolor.  Ectomycorrhizal fungi are soil-borne mutualistic organisms whose hyphae wrap around host roots and grow into the root apoplastic space where the fungus exchanges nutrients such as nitrogen and phosphorus in return for plant derived sugars.  The MiSSP7 protein is induced by root exudates and is highly expressed throughout the root colonization process.  Its presence was responsible for alterations to the plant transcriptomic profile, a mechanism by which MiSSP7 may aid in the formation of the symbiotic interface. Here we further discuss the implications of these findings and, further, we demonstrate that the production of MiSSP7 is induced by two flavonoids, rutin and quercitrin, a class of compounds normally found within the exudates of plant roots.  We also consider the interesting similarities between the mechanisms of effector induction and action between pathogenic and mutualistic fungi.  

  9. Chemical composition and antifungal activities of essential oils of Satureja thymbra L. and Salvia pomifera ssp. calycina (Sm.) Hayek

    NARCIS (Netherlands)

    Glamoclija, J.; Sokovic, M.; Vukojevic, J.; Milenkovic, I.; Griensven, van L.J.L.D.

    2006-01-01

    This work covers the chemical composition and antifungal activities of essential oils isolated from savory (Satureja thymbra) and sage (Salvia pomifera ssp. calycina) analyzed using GC/MS. The main components of S. thymbra oil were gamma-terpinene (23.2%) and carvacrol (48.5%). The main components i

  10. Introgression of genetic material from Zea mays ssp. Mexicana into cultivated maize was facilitated by tissue culture

    International Nuclear Information System (INIS)

    Zea mays ssp. mexicana, a wild relative of cultivated maize (Z. mays ssp. mays), is a useful gene resource for maize breeding. In this study, two populations were generated by conventional breeding scheme (population I) or tissue culture regime (population II), respectively, to introgress genetic material of Z. mays ssp. mexicana into maize. Karyotype analysis showed that the arm ratios of 10 pairs of chromosomes in parent maize Ye515 and derivative lines from 2 different populations with 26% and 38% chromosome variation frequencies, respectively. Alien chromatin was detected in the root tip cells of progeny plants through genomic in situ hybridization (GISH). There were 3.3 chromosomes carrying alien chromatin on average in population I and 6.5 in population II. The hybridization signals were located mainly at the terminal or sub terminal regions of the chromosomes and the sizes were notably variant among lines. Based on those results, it is concluded that the introgression of genetic material from Z. mays ssp. mexicana into cultivated maize was facilitated by tissue culture, and subsequently some excellent materials for maize breeding were created. (author)

  11. Fate of Clavibacter michiganensis ssp. sepedonicus, the causal organism of bacterial ring rot in potato, in weeds and field crops.

    NARCIS (Netherlands)

    Wolf, van der J.M.; Beckhoven, van J.R.C.M.; Hukkanen, A.; Karjalainen, R.; Muller, P.

    2005-01-01

    Crops and weeds were tested for their ability to host Clavibacter michiganensis ssp. sepedonicus (Cms), the causal agent of bacterial ring rot in potato. Ten crops grown in rotation with potato in Europe, namely maize, wheat, barley, oat, bush bean, broad bean, rape, pea and onion and five cultivars

  12. Molecular genetic tagging of Beta vulgaris ssp. maritima-derived resistance to the sugar beet cyst nematode, Heterodera schachtii

    Science.gov (United States)

    Resistance in commercial sugar beet hybrids to the sugar beet cyst nematode (SBCN) principally has been based on the Hs1 gene from the wild beet Beta procumbens, yet incorporation of this resistance has been detrimental to crop yield in nematode-free fields. Accessions of B. vulgaris ssp maritima w...

  13. Full Scale Test SSP 34m blade, edgewise loading LTT. Extreme load and PoC_InvE Data report

    DEFF Research Database (Denmark)

    Nielsen, Magda; Roczek-Sieradzan, Agnieszka; Jensen, Find Mølholt;

    This report is the second report covering the research and demonstration project “Eksperimentel vingeforskning: Strukturelle mekanismer i nutidens og fremtidens store vinger under kombineret last”, supported by the EUDP program. A 34m wind turbine blade from SSP-Technology A/S has been tested...

  14. The response of Plantago major ssp pleiosperma to elevated CO2 is modulated by the formation of secondary shoots

    NARCIS (Netherlands)

    Fonseca, F; DenHertog, J; Stulen, [No Value

    1996-01-01

    The effect of elevated CO2 on the relative growth rate (RGR) of Plantago major ssp. pleiosperma was studied during the vegetative stage, in relation to plant development, by growing plants at 350 mu l l(-1) or at 700 mu l l(-1) CO2 in non-limiting nutrient solution with nitrate. To minimize interfer

  15. A black, non-troglomorphic amphibian from the karst of Slovenia: Proteus anguinus parkelj n. ssp. (Urodela: Proteidae)

    NARCIS (Netherlands)

    Sket, B.; Arntzen, J.W.

    1994-01-01

    A morphologically distinct cavernicolous salamander Proteus anguinus from southeastern Slovenia (Bela Krajina) is described as P. a. parkelj ssp. n. It differs from P. a. anguinus in a dark pigmentation, fully developed eyes, a skull with broader and shorter bones and fewer teeth, a voluminous jaw m

  16. CITOGENETICS EFFECTS INDUCED BY THE ASCORBIC ACID TREATMENT OF LARIX DECIDUA MILL. SSP. CARPATICA AND PICEA ABIES (L. KARST

    Directory of Open Access Journals (Sweden)

    Ioana Ieremia

    2006-08-01

    Full Text Available The paper present the influence of ascorbic acid upon the mitotic division of Larix decidua Mill ssp. carpatica and Picea abies (L. Karst. The treatment is applied of two variants, germinated seed in ascorbic acid (variantAand germinated seeds in disttilate water, than treated with ascorbic acid in 3 concentrations (variant B.

  17. Effect of a switch from elevated to ambient CO2 on growth and carbohydrate allocation of Plantago major ssp pleiosperma

    NARCIS (Netherlands)

    Fonseca, F; Stulen, [No Value

    2000-01-01

    Transfer of plants of Plantago major ssp. pleiosperma (L.) Pilger to elevated CO2 (700 mul l(-1)) showed a transient stimulation of the relative growth rate (RGR). Thereafter the RGR of the plants grown at elevated CO2 was the same as for plants grown at ambient CO2 (350 mul l(-1)). At that time sta

  18. Engineered Lactobacillus rhamnosus GG expressing IgG-binding domains of protein G: Capture of hyperimmune bovine colostrum antibodies and protection against diarrhea in a mouse pup rotavirus infection model.

    Science.gov (United States)

    Günaydın, Gökçe; Zhang, Ran; Hammarström, Lennart; Marcotte, Harold

    2014-01-16

    Rotavirus-induced diarrhea causes more than 500,000 deaths annually in the world, and although vaccines are being made available, new effective treatment strategies should still be considered. Purified antibodies derived from hyperimmune bovine colostrum (HBC), from cows immunized with rotavirus, were previously used for treatment of rotavirus diarrhea in children. A combination of HBC antibodies and a probiotic strain of Lactobacillus (L. rhamnosus GG) was also found to be more effective than HBC alone in reducing diarrhea in a mouse model of rotavirus infection. In order to further improve this form of treatment, L. rhamnosus GG was engineered to display surface expressed IgG-binding domains of protein G (GB1, GB2, and GB3) which capture HBC-derived IgG antibodies (HBC-IgG) and thus target rotavirus. The expression of IgG-binding domains on the surface of the bacteria as well as their binding to HBC-IgG and to rotavirus (simian strain RRV) was demonstrated by Western blot, flow cytometry, and electron microscopy. The prophylactic effect of engineered L. rhamnosus GG and anti-rotaviral activity of HBC antibodies was evaluated in a mouse pup model of RRV infection. The combination therapy with engineered L. rhamnosus GG (PG3) and HBC was significantly more effective in reducing the prevalence, severity, and duration of diarrhea in comparison to HBC alone or a combination of wild-type L. rhamnosus GG and HBC. The new therapy reduces the effective dose of HBC between 10 to 100-fold and may thus decrease treatment costs. This antibody capturing platform, tested here for the first time in vivo, could potentially be used to target additional gastrointestinal pathogens. PMID:24291196

  19. In vitro L.casei EPS promote small intestinal epithelial cells of Balb/c mice proliferation and secretion of IL-8, IL-10 and TGF-β%L.casei EPS体外促进Balb/c小鼠小肠上皮细胞增殖及其分泌IL-8、IL-10和TGF-β

    Institute of Scientific and Technical Information of China (English)

    霍思序; 唐彦君; 刘宁

    2013-01-01

    目的 以干酪乳杆菌(Lactobacillus casei,L.casei)胞外多糖(exopolysaccharides,EPS)为研究对象,研究其在体外对Balb/c小鼠小肠上皮细胞(small intestinal epithelial cell,IECs)增殖及其分泌IL-8、IL-10和TGF-β的影响.方法 利用嗜热菌蛋白酶消化联合差速贴壁以及免疫细胞化学鉴定的方法对Balb/c小鼠IECs进行分离和鉴定,获得实验用的IECs.用不同质量浓度的L.caseiEPS与IECs共培养,分别观察0.5、1、2h和4h后IECs的增殖情况.当用不同质量浓度的L.casei EPS处理IECs 2 h后,采用双抗体夹心ELISA法检测细胞培养上清液中细胞因子IL-8、IL-10和TGF-β的质量浓度.结果 嗜热菌蛋白酶消化联合差速贴壁的方法能够获得纯度高与活性好的IECs;L.casei EPS能够促进IECs的增殖,显著上调上清液中IL-8、IL-10和TGF-β的分泌水平(P<0.05),EPS质量浓度在0~150 μg/ml范围内呈剂量依赖关系,并且在150 μg/ml的质量浓度时达到最大值.结论 L.casei EPS能够促进IECs的增殖及IL-8、IL-10和TGF-β的分泌水平.

  20. Effect of inoculative proportion of probiotic Lactobacillus casei Zhang , Bifidobacterium lactis V9 and milk solids content on their viable counts in fermented milk%益生菌Lactobacillus casei Zhang和Bifidobacterium lactis V9接种比例及乳固形物含量对其活菌数的影响

    Institute of Scientific and Technical Information of China (English)

    其木格苏都; 王记成; 孟和毕力格; 张和平

    2012-01-01

    Lactobacillus casei Zhang and Bifidobacterium lactis V9 were inoculated with different proportion (1:3, 1:1 and 3:l)into skim bovine milk of different solid contents (10, 12, 14 and 16%) for fermentation at 37 ℃ till pH 4.5 and subsequently storaged for 28 d at 4 ℃. And then the viable counts of these two strains during storage were checked. The results showed that the higher viable counts of L. casei Zhang and B. lactis V9 obtained when two bacteria with 3:1 proportion inoculation in the groups with same solid content. After the storage, the higher survival rate of L. casei Zhang and B. lactis V9 was observed when solid content was 14% in the groups with same proportion. This research indicated that the inoculative proportion of these two bacteria and the solid content in raw milk play very important roles in the growth and survive ability of L. casei Zhang and B. lactis V9.%将益生菌Lactobacillus casei Zhang和Bifidobacterium lactis V9按1∶3、1∶1、3∶1(总接种量为2×106cfu/g)接种于总固形物含量为10%、12%、14%、16%的脱脂牛乳中,37℃恒温发酵至pH 4.5,于4℃贮藏28 d.测定发酵及贮藏结束2株益生菌活菌数.试验结果表明,发酵结束,当研究固形物质量分数相同,接种比例为3∶1时,L.casei Zhang和B.lactis V9的活菌数最高.贮藏结束,当接种比例相同,所含乳固形物质量分数为14%时,两株益生菌存活率显著高于其他固形物含量组(p<0.05).本研究显示,L.asei Zhang和B.lactis接种比例为3∶1,乳固形物质量分数为14%时,两株益生菌生长良好且存活率高,适宜生产应用.

  1. Aspectos ecológicos y distribución de Tropaeolum tuberosum ssp. silvestre (Tropaeolaceae en Argentina

    Directory of Open Access Journals (Sweden)

    Eva Bulacio

    2012-06-01

    Full Text Available Tropaeolum tuberosum Ruiz et Pav. ssp. silvestre Sparre es una hierba rizomatosa trepadora que en Argentina fue encontrada en las Sierras de Calilegua (Jujuy y en áreas montanas de Catamarca, aunque de esta última localidad sólo se conoce un único e incompleto ejemplar. Habita quebradas húmedas, entre los 2.100 y 2.650 m s.n.m., en el límite superior del bosque montano. El tallo es grácil de hasta 3 m de largo y la porción subterránea está constituida por rizomas alargados y de hasta 1 cm de diámetro. Estas son las principales diferencias con la ssp. tuberosum, en la cual el tallo es más corto, erguido y desarrolla tubérculos bien definidos.Ecological features and distribution of Tropaeolum tuberosum ssp. silvestre (Tropaeolaceae in Argentina. Tropaeolum tuberosum Ruiz et Pav. ssp. silvestre Sparre is a rhizomatous climbing herb that in Argentina was found in the Sierras of Calilegua (Jujuy and Catamarca mountains, yet of this last locality only is known an one and incomplete specimen. It inhabits humid gorges, between 2100-2650 masl, in the upper limit of the montane forest. The slender stem reaches 3 meters in lenght and the underground portion shows elongated rhizomes up to 1 cm in diameter. These are the main differences with the ssp. tuberosum, in which the stem is shorter, erect and develop well-defned tubers.

  2. Identification of a small molecule that modifies MglA/SspA interaction and impairs intramacrophage survival of Francisella tularensis.

    Directory of Open Access Journals (Sweden)

    Algevis P Wrench

    Full Text Available The transcription factors MglA and SspA of Francisella tularensis form a heterodimer complex and interact with the RNA polymerase to regulate the expression of the Francisella pathogenicity island (FPI genes. These genes are essential for this pathogen's virulence and survival within host cells. In this study, we used a small molecule screening to identify quinacrine as a thermal stabilizing compound for F. tularensis SCHU S4 MglA and SspA. A bacterial two-hybrid system was used to analyze the in vivo effect of quinacrine on the heterodimer complex. The results show that quinacrine affects the interaction between MglA and SspA, indicated by decreased β-galactosidase activity. Further in vitro analyses, using size exclusion chromatography, indicated that quinacrine does not disrupt the heterodimer formation, however, changes in the alpha helix content were confirmed by circular dichroism. Structure-guided site-directed mutagenesis experiments indicated that quinacrine makes contact with amino acid residues Y63 in MglA, and K97 in SspA, both located in the "cleft" of the interacting surfaces. In F. tularensis subsp. novicida, quinacrine decreased the transcription of the FPI genes, iglA, iglD, pdpD and pdpA. As a consequence, the intramacrophage survival capabilities of the bacteria were affected. These results support use of the MglA/SspA interacting surface, and quinacrine's chemical scaffold, for the design of high affinity molecules that will function as therapeutics for the treatment of Tularemia.

  3. Full scale test SSP 34m blade, combined load. Data report

    Energy Technology Data Exchange (ETDEWEB)

    Nielsen, Per H.; Nielsen, Magda; Jensen, Find M. (and others)

    2010-11-15

    This report is part of the research project where a 34m wind turbine blade from SSP-Technology A/S was tested in combined flap and edgewise load. The applied load is 55% of an imaginary extreme event based on the certification load of the blade. This report describes the reason for choosing the loads and the load direction and the method of applying the loads to the blade. A novel load introduction allows the blade to deform in a more realistic manner, allowing the observation of e.g. transverse shear distortion. The global and local deformation of the blade as well as the blades' respond to repeated tests has been studied and the result from these investigations are presented, including the measurements performed. (Author)

  4. Combining ability for maturity and plant height in brassica rapa (l.) ssp. dichotoma (roxb.) hanelt

    International Nuclear Information System (INIS)

    A 5 * 5 F1 diallel cross hybrids of Brassica rapa (L.) ssp. dichotoma (Roxb.) Hanelt along with parents were evaluated through combining ability for days to flowering (initiation and completion), days to maturity and plant height. Highly significant differences were recorded for all the traits. Mean squares due to general, specific and reciprocal combining ability were significant for all the traits except plant height for which the latter two components were non-significant. Prevalence of additive (plant height), non-additive (days to flowering completion; days to maturity) and reciprocal effects (days to flowering initiation) were detected. Parental line G-403 was best general combiner for all the traits. The F1 hybrids G-902 * G-265 (days to flowering initiation), G-902 * G-403 (days to flowering completion), G-265 * G-1500 (days to maturity) and G-909 * G-265 (plant height) were superior and may be exploited for future breeding programs. (author)

  5. Herbaspirillum sp. strain GW103 alleviates salt stress in Brassica rapa L. ssp. pekinensis.

    Science.gov (United States)

    Lee, Gun Woong; Lee, Kui-Jae; Chae, Jong-Chan

    2016-05-01

    Mutual interactions between plant and rhizosphere bacteria facilitate plant growth and reduce risks of biotic and abiotic stresses. The present study demonstrates alleviation of salt stress in Brassica rapa L. ssp. perkinensis (Chinese cabbage) by Herbaspirillum sp. strain GW103 isolated from rhizosphere soil of Phragmites australis. The strain was capable of producing plant beneficial factors, such as auxin, siderophore, and 1-aminocylopropane-1-carboxylic acid deaminase. Treatment of strain GW103 on Chinese cabbage under salt stress increased K(+)/Na(+) ratio in roots generating balance in the ratio of ion homeostasis and consequently contributed to the increase of biomass. In addition, root colonization potential of the strain was observed by green fluorescent protein (GFP)-tagging approach. These results strongly suggest the beneficial impact of strain GW103 by inducing the alleviation of salt stress and development of stress tolerance in Chinese cabbage via plant-microbe interaction. PMID:26358119

  6. Acetylcholinesterase inhibitory activity of lycopodane-type alkaloids from the Icelandic Lycopodium annotinum ssp. alpestre

    DEFF Research Database (Denmark)

    Halldórsdóttir, Elsa Steinunn; Jaroszewski, Jerzy W; Olafsdottir, Elin Soffia

    2010-01-01

    The aim of this study was to investigate structures and acetylcholinesterase inhibitory activities of lycopodane-type alkaloids isolated from an Icelandic collection of Lycopodium annotinum ssp. alpestre. Ten alkaloids were isolated, including annotinine, annotine, lycodoline, lycoposerramine M......, anhydrolycodoline, gnidioidine, lycofoline, lannotinidine D, and acrifoline, as well as a previously unknown N-oxide of annotine. (1)H and (13)C NMR data of several of the alkaloids were provided for the first time. Solvent-dependent equilibrium constants between ketone and hemiketal form of acrifoline were...... determined. Conformation of acrifoline was characterized using NOESY spectroscopy and molecular modelling. The isolated alkaloids were evaluated for their in vitro inhibitory activity against acetylcholinesterase and butyrylcholinesterase. Ligand docking studies based on mutated 3D structure of Torpedo...

  7. The identification of MacSe in Streptococcus equi ssp. equi

    Institute of Scientific and Technical Information of China (English)

    Jiande YANG; Yanfei LIU; Jun XU; Jifei MA

    2009-01-01

    Streptococcus equi subsp, equi (S. equi ssp. equi) causes equine strangles, a highly contagious and widespread purulent lymphadenitis of the head and neck. We have identified MacSe, a novel protein of S. equi, by screening a phage library of 3-8 kb random DNA fragments of S. equi CF32. MacSe shares 62% and 67.5% amino acid homology with Mac5005 and Mac8345 of S. pyogenes respectively. Expression during infection was shown by strong reactivity of the protein with convalescent sera and mucosal wash IgA of ponies infected by commingling exposure. Release into the culture medium was detected during the log phase of growth. Dose dependent anti-phagocytic activity for equine neutrophils involved interaction of MacSe with C3 and neutrophils.

  8. Correction of the structure of a new sesquiterpene from Cistus creticus ssp. creticus.

    Science.gov (United States)

    Hatzellis, Konstantinos; Pagona, Georgia; Spyros, Apostolos; Demetzos, Costas; Katerinopoulos, Haralambos E

    2004-12-01

    In an attempt to identify the structure of a sesquiterpene from Cistus creticus ssp. creticus proposed in the literature as 1,1,4a,6-tetramethyl-5-methylene-1,2,3,4,4alpha,5,8,8alpha-octahydronaphthalene, the synthesis of its cis isomer 2 was carried out in 11 steps and 9.5% yield. Comparison of the spectra of 2 and those reported earlier for the synthetic trans isomer 1 with the spectral profile of the isolated natural product indicated that the latter was not compatible with either 1 or 2. The correct structure was assigned, by detailed spectroscopic analysis of the natural product, as 6-isopropenyl-4,4a-dimethyl-1,2,3,4,4a,5,6,7-octahydronaphthalene (3). PMID:15620240

  9. Lignan formation in hairy root cultures of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter)

    Science.gov (United States)

    Wawrosch, Christoph; Schwaiger, Stefan; Stuppner, Hermann; Kopp, Brigitte

    2014-01-01

    A hairy root line of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter) was obtained upon transformation with Agrobacterium rhizogenes strain ATCC15834. Elicitation of this line with silver nitrate, sucrose, methyl jasmonate and yeast extract at various concentrations in most cases resulted in a stimulation of lignan biosynthesis. Through elicitation with 6% sucrose the roots accumulated the pharmacologically active lignans leoligin and 5-methoxy-leoligin at levels of 0.0678% and 0.0372%, respectively, without significant growth inhibition. These lignan levels were comparable to those found in intact roots of cultivated Edelweiss. The biotechnological production of leoligin could be an attractive option for the continuous, field culture-independent production of the valuable secondary metabolites leoligin and 5-methoxy-leoligin. PMID:24932777

  10. Antioxidant capacities and polyphenolics of Chinese cabbage (Brassica rapa L. ssp. Pekinensis) leaves.

    Science.gov (United States)

    Seong, Gi-Un; Hwang, In-Wook; Chung, Shin-Kyo

    2016-05-15

    Chinese cabbage (Brassica rapa L. ssp. Pekinensis) is a green leafy vegetable used mainly in kimchi, salted and fermented dishes. Consumer preference for the leaf portion differs according to the type of dishes. In this study, Chinese cabbage was divided into three parts, and their antioxidant activities were investigated through in vitro assays. The total phenolic contents (TPC), total flavonoid contents (TFC), and vitamin C contents were also determined as indicators of antioxidant contents. The phenolic acids and flavonoids were separated and identified using high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). The outer leaf had the strongest antioxidant activity with the maximum antioxidant contents, followed by the mid- and inner leaves. Principal component analysis (PCA) revealed that outer leaf is positively related to caffeic acid, p-coumaric acid, ferulic acid, and myricetin contents, whereas the mid- and inner leaves are negatively related to sinapic acid contents. PMID:26776015

  11. meta-Tyrosine in Festuca rubra ssp. commutata (Chewings fescue) is synthesized by hydroxylation of phenylalanine.

    Science.gov (United States)

    Huang, Tengfang; Rehak, Ludmila; Jander, Georg

    2012-03-01

    m-Tyrosine is a non-protein amino acid that is structurally similar to the common protein amino acids p-tyrosine and phenylalanine. Copious amounts of m-tyrosine can be found in root exudates of the fine fescue cultivar, Festuca rubra L. ssp. commutata (Chewings fescue). The phytotoxicity of m-tyrosine may contribute to the allelopathic potential of F. rubra. m-Tyrosine in Euphorbia myrsinites (donkey-tail spurge), was previously shown to be synthesized via transamination of m-hydroxyphenylpyruvate. Here we show that m-tyrosine biosynthesis in F. rubra occurs through direct hydroxylation of phenylalanine in the root tips, perhaps through the activity of a cytochrome P450 enzyme. Hence, E. myrsinites and F. rubra, the only two plant species known to produce m-tyrosine, use distinct biosynthetic pathways that likely arose independently in evolutionary history.

  12. A food-grade fimbrial adhesin FaeG expression system in Lactococcus lactis and Lactobacillus casei.

    Science.gov (United States)

    Lu, W W; Wang, T; Wang, Y; Xin, M; Kong, J

    2016-03-01

    Enterotoxigenic Escherichia coli (ETEC) infection is the major cause of diarrhea in neonatal piglets. The fimbriae as colonizing factor in the pathogenesis of ETEC constitute a primary target for vaccination against ETEC. Lactic acid bacteria (LAB) are attractive tools to deliver antigens at the mucosal level. With the safety of genetically modified LAB in mind, a food-grade secretion vector (pALRc or pALRb) was constructed with DNA entirely from LAB, including the replicon, promoter, signal peptide, and selection marker alanine racemase gene (alr). To evaluate the feasibility of the system, the nuclease gene (nuc) from Staphylococcus aureus was used as a reporter to be expressed in both Lactococcus lactis and Lactobacillus casei. Subsequently, the extracellular secretion of the fimbrial adhesin FaeG of ETEC was confirmed by Western blot analysis. These results showed that this food-grade expression system has potential as the delivery vehicle for the safe use of genetically modified LAB for the development of vaccines against ETEC infection.

  13. A food-grade fimbrial adhesin FaeG expression system in Lactococcus lactis and Lactobacillus casei.

    Science.gov (United States)

    Lu, W W; Wang, T; Wang, Y; Xin, M; Kong, J

    2016-03-01

    Enterotoxigenic Escherichia coli (ETEC) infection is the major cause of diarrhea in neonatal piglets. The fimbriae as colonizing factor in the pathogenesis of ETEC constitute a primary target for vaccination against ETEC. Lactic acid bacteria (LAB) are attractive tools to deliver antigens at the mucosal level. With the safety of genetically modified LAB in mind, a food-grade secretion vector (pALRc or pALRb) was constructed with DNA entirely from LAB, including the replicon, promoter, signal peptide, and selection marker alanine racemase gene (alr). To evaluate the feasibility of the system, the nuclease gene (nuc) from Staphylococcus aureus was used as a reporter to be expressed in both Lactococcus lactis and Lactobacillus casei. Subsequently, the extracellular secretion of the fimbrial adhesin FaeG of ETEC was confirmed by Western blot analysis. These results showed that this food-grade expression system has potential as the delivery vehicle for the safe use of genetically modified LAB for the development of vaccines against ETEC infection. PMID:26825016

  14. Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota

    Directory of Open Access Journals (Sweden)

    Elizabeth R. Mann

    2013-01-01

    Full Text Available Background. Dendritic cells regulate immune responses to microbial products and play a key role in ulcerative colitis (UC pathology. We determined the immunomodulatory effects of probiotic strain Lactobacillus casei Shirota (LcS on human DC from healthy controls and active UC patients. Methods. Human blood DC from healthy controls (control-DC and UC patients (UC-DC were conditioned with heat-killed LcS and used to stimulate allogeneic T cells in a 5-day mixed leucocyte reaction. Results. UC-DC displayed a reduced stimulatory capacity for T cells (P<0.05 and enhanced expression of skin-homing markers CLA and CCR4 on stimulated T cells (P<0.05 that were negative for gut-homing marker β7. LcS treatment restored the stimulatory capacity of UC-DC, reflecting that of control-DC. LcS treatment conditioned control-DC to induce CLA on T cells in conjunction with β7, generating a multihoming profile, but had no effects on UC-DC. Finally, LcS treatment enhanced DC ability to induce TGFβ production by T cells in controls but not UC patients. Conclusions. We demonstrate a systemic, dysregulated DC function in UC that may account for the propensity of UC patients to develop cutaneous manifestations. LcS has multifunctional immunoregulatory activities depending on the inflammatory state; therapeutic effects reported in UC may be due to promotion of homeostasis.

  15. VizieR Online Data Catalog: SSP in NIR. II. Synthesis models (Meneses-Goytia+, 2015)

    Science.gov (United States)

    Meneses-Goytia, S.; Peletier, R. F.; Trager, S. C.; Vazdekis, A.

    2015-08-01

    The present Single Stellar Populations (SSP) models are derived from my Ph.D.'s thesis and this paper. The following nomenclature is used throughout the paper and the website (http://smg.astro-research.net/ssp-models/the-models/) to describe the models, e.g. MarS models use the M08 isochrones (Mar) and the Salpeter (S) IMF. General information about the models is given in table1. For further information, please refer to the paper. Each set of models and their corresponding predictions are available in the website and VIZIER. The spectral energy distributions (SEDs) can be downloaded in a zip-file from those pages in ascii format . The spectral energy distributions (SEDs) can be downloaded in a zip-file from t hose pages in ascii format . The nomenclature of each SED is as follows: isochroneIMFsedXXXXHZX.XXXXXXXXXTgXX.XXXXXXXe+XX - where XXXX tells whether those models contain C-stars or no (COMBO or NOCS respectively) H is the spectral band in which normalization occurred Z_X.XXXXXXXXX is the metallicity in terms of Z Tg_XX.XXXXXXXe+XX is the age in years. Each set of models contains MarS - 96 SEDs GirS - 96 SEDs BaSS - 116 SEDs We have also included in the websites the Integrated colours and line-strength indices from all our models (MarS, GirS and BaSS). The SEDs were convolved to a velocity dispersion of 350km/s before calculating indices. (5 data files).

  16. Action of lactoferrin on the multiplication of Lactobacillus casei in vitro and in Minas fresh cheeseAtividade antimicrobiana; Atividade biológica; Queijo; Probióticos; Produtos lácteos.

    Directory of Open Access Journals (Sweden)

    Lina Casale Aragon-Alegro

    2012-02-01

    Full Text Available This study evaluated the activity of lactoferrin on the multiplication of probiotic Lactobacillus casei in vitro and in Minas fresh cheese. Growth curves of L. casei in BHI broth containing 1 mg/mL and 2 mg/mL of lactoferrin were performed. Additionally, Minas fresh cheeses added of L. casei (control and added of L. casei and lactoferrin (2 mg/g and 4 mg/g were produced and stored at 5°C during 28 days. Cheeses were analyzed for pH, titratable acidity and enumeration of L. casei and psychotropic microorganisms in days 1, 7, 14, 21 and 28 and for the centesimal composition in day 1. The experiment was repeated three times, and data were analyzed by ANOVA and Tukey test at 5% significance level. When tested in vitro, L. casei multiplication was stimulated by lactoferrin at a concentration of 2 mg/mL, but this activity has not been verified in the cheese, even in that added by lactoferrin at 4 mg/g. Psychotropic population in the cheeses added by lactoferrin did not differ from control cheese (P>0.05, demonstrating that no antimicrobial activity occurred in the products. More studies should be performed to evaluate the antimicrobial effects of lactoferrin in foods, since they have more variables that may affect the activity of this protein when comparing to in vitro tests.O presente estudo avaliou a atividade da lactoferrina sobre a multiplicação de Lactobacillus casei probiótico in vitro e no queijo Minas frescal. As curvas de crescimento de L. casei em caldo BHI contendo lactoferrina nas concentrações de 1 mg/mL and 2 mg/mL foram construídas. Adicionalmente, os queijos Minas frescal contendo somente L. casei (controle e L. casei e a lactoferrina (2 mg/g e 4 mg/g foram produzidos e armazenados a 5°C durante 28 dias. Foram realizadas as análises de pH, acidez livre titulável e enumeração de L. casei e microrganismos psicrotróficos no dia 1 (dia da produção e após 7, 14, 21 e 28 dias de armazenamento. Além disso, no dia 1, foram

  17. No evidence of harms of probiotic Lactobacillus rhamnosus GG ATCC 53103 in healthy elderly-a phase I open label study to assess safety, tolerability and cytokine responses.

    Directory of Open Access Journals (Sweden)

    Patricia L Hibberd

    Full Text Available BACKGROUND: Although Lactobacillus rhamnosus GG ATCC 53103 (LGG has been consumed by 2 to 5 million people daily since the mid 1990s, there are few clinical trials describing potential harms of LGG, particularly in the elderly. OBJECTIVES: The primary objective of this open label clinical trial is to assess the safety and tolerability of 1×1010 colony forming units (CFU of LGG administered orally twice daily to elderly volunteers for 28 days. The secondary objectives were to evaluate the effects of LGG on the gastrointestinal microbiome, host immune response and plasma cytokines. METHODS: Fifteen elderly volunteers, aged 66-80 years received LGG capsules containing 1×1010 CFU, twice daily for 28 days and were followed through day 56. Volunteers completed a daily diary, a telephone call on study days 3, 7 and 14 and study visits in the Clinical Research Center at baseline, day 28 and day 56 to determine whether adverse events had occurred. Assessments included prompted and open-ended questions. RESULTS: There were no serious adverse events. The 15 volunteers had a total of 47 events (range 1-7 per volunteer, 39 (83% of which were rated as mild and 40% of which were considered related to consuming LGG. Thirty-one (70% of the events were expected, prompted symptoms while 16 were unexpected events. The most common adverse events were gastrointestinal (bloating, gas, and nausea, 27 rated as mild and 3 rated as moderate. In the exploratory analysis, the pro-inflammatory cytokine interleukin 8 decreased during LGG consumption, returning towards baseline one month after discontinuing LGG (p = 0.038 while there was no difference in other pro- or anti-inflammatory plasma cytokines. CONCLUSIONS: Lactobacillus rhamnosus GG ATCC 53103 is safe and well tolerated in healthy adults aged 65 years and older. TRIAL REGISTRATION: ClinicalTrials.gov NCT 01274598.

  18. Lipopolysaccharide-Induced Profiles of Cytokine, Chemokine, and Growth Factors Produced by Human Decidual Cells Are Altered by Lactobacillus rhamnosus GR-1 Supernatant.

    Science.gov (United States)

    Li, Wei; Yang, Siwen; Kim, Sung O; Reid, Gregor; Challis, John R G; Bocking, Alan D

    2014-01-15

    The aim of this study was to assess the effects of bacterial lipopolysaccharide (LPS) and Lactobacillus rhamnosus GR-1 supernatant (GR-1SN) on secretion profiles of cytokines, chemokines, and growth factors from primary cultures of human decidual cells. Lipopolysaccharide significantly increased the output of proinflammatory cytokines (interleukin [IL]-1B, IL-2, IL-6, IL-12p70, IL-15, IL-17A, interferon gamma [IFN-γ], and tumor necrosis factor [TNF]); anti-inflammatory cytokines (IL-1RN, IL-4, IL-9, and IL-10); chemokines (IL-8, eotaxin, IFN-inducible protein 10 [IP-10], monocyte chemoattractant protein 1 [MCP-1], macrophage inflammatory protein-1α [MIP-1α], macrophage inflammatory protein-1β [MIP-1β], and regulated on activation normal T cell expressed and secreted [RANTES]); and growth factors (granulocyte colony-stimulating factor [CSF] 3, CSF-2, and vascular endothelial growth factor A [VEGFA]). Lactobacillus rhamnosus GR-1SN alone significantly increased CSF-3, MIP-1α MIP-1β, and RANTES but decreased IL-15 and IP-10 output. The GR-1SN also significantly or partially reduced LPS-induced proinflammatory cytokines TNF, IFN-γ, IL-1β, IL-2 IL-6, IL-12p70, IL-15, IL-17, and IP-10; partially reduced LPS-induced anti-inflammatory cytokines IL-1RN, IL-4 and IL-10, and LPS-induced VEGFA output but did not affect CSF-3, MIP-1α, MIP-1β, MCP-1, IL-8, and IL-9. Our results demonstrate that GR-1SN attenuates the inflammatory responses to LPS by human decidual cells, suggesting its potential role in ameliorating intrauterine infection. PMID:24429676

  19. Modulación de la fisiología gastrointestinal mediante cepas probióticas de Lactobacillus casei y Bifidobacterium bifidum Modulation of gastrointestinal physiology through probiotic strains of Lactobacillus casei and Bifidobacterium bifidum

    Directory of Open Access Journals (Sweden)

    J. Barrenetxe

    2006-12-01

    Full Text Available En el contexto de la alimentación y la promoción de la salud se sitúan los productos denominados alimentos funcionales que tienen diversos efectos beneficiosos en el organismo, además de los meramente nutricionales. Dentro de estos alimentos funcionales, entre otros, podemos distinguir entre compuestos probióticos y prebióticos. Los microorganismos más utilizados en alimentos probióticos pertenecen a los géneros Lactobacillus y Bifidobacterium. En el presente trabajo se ha estudiado el efecto de dietas suplementadas con Lactobacillus casei o Bifidobacterium bifidum en el desarrollo animal y en especial sobre la función intestinal, centrada en su actividad immunitaria, digestiva y absortiva de animales en crecimiento. Las cepas bacterianas utilizadas modifican la actividad del intestino delgado de los ratones sanos, afectando significativamente a su actividad enzimática (sacarasa, maltasa y aminopeptidasa y a la captación de nutrientes (galactosa y glicilsarcosina, así como a la actividad inmune intestinal (mayor número de placas de Peyer. Sin embargo, estos efectos no parecen perturbar el desarrollo de los animales en crecimiento ya que no se aprecian diferencias significativas en su peso corporal ni en sus parámetros sanguíneos. Estos resultados ponen de manifiesto los posibles efectos beneficiosos en la fisiología intestinal y contribuyen al conocimiento de los posibles mecanismos de acción de los probióticos, que se pudieran utilizar en el tratamiento preventivo de diferentes patologías relacionadas con el aparato digestivo.The products called functional foods, which besides being merely nutritional have different beneficial effects on the organism, are situated in the context of diet and health promotion. Amongst these functional foods we can distinguish, amongst others, between probiotic and prebiotic compounds. The micro-organisms most widely used in probiotic foods belong to the Lactobacillus and Bifidobacteriumtypes

  20. Study on the stability of the probiotic Lactobacillus casei Zhang during continuous subculturing in normal condition for 1000 generations%益生菌Lactobacillus casei Zhang在常规培养条件下连续传代1000代过程中稳定性研究

    Institute of Scientific and Technical Information of China (English)

    张文羿; 白梅; 张和平

    2013-01-01

    以益生菌Lactobacillus casei Zhang为研究对象,通过观察该菌株在普通MRS培养基中连续传代1 000代期间细胞形态、菌落形态、活菌数、浊度和菌株活力的变化对其稳定性进行了初步评价.结果表明,L.casei Zhang在1000代期间细胞形态与菌落形态没有发生变化;活菌数、浊度均和菌株活力随培养代数的增加呈现波动性变化,但波动幅度较小,总数基本维持不变.上述研究结果初步表明L casei Zhang具有良好的稳定性,暗示其可用于产业化生产.

  1. Effects of Probiotic Lactobacillus Casei DN-114 001 in Prevention of Radiation-Induced Diarrhea: Results From Multicenter, Randomized, Placebo-Controlled Nutritional Trial

    International Nuclear Information System (INIS)

    Purpose: To determine whether a probiotic drink containing Lactobacillus casei DN-114 001 reduces the incidence of radiation-induced diarrhea in patients with gynecologic cancer. Methods and Materials: Patients who were undergoing pelvic radiotherapy (45-50 Gy, conventional fractionation) for either cervical carcinoma (radiotherapy and weekly cisplatin) or endometrial adenocarcinoma (postoperative radiotherapy) were randomly assigned to a probiotic drink or placebo, in a double-blind fashion. The probiotic drink consisted of liquid yogurt containing L. casei DN-114 001 at 108 CFU/g. The patients recorded the daily the number of bowel movements and scored the stool consistency using the Bristol scale. Diarrhea was graded weekly according the Common Toxicity Criteria system. The primary endpoint was to reduce the incidence of diarrhea, defined by a Common Toxicity Criteria Grade of 2 or greater or the need for loperamide. Results: A total of 85 patients were enrolled. Grade 2 or greater diarrhea and/or the use of loperamide was observed in 24 of 41 patients in the placebo group and 30 of 44 in the probiotic group (p = 0.568). No differences were found in the median time to the presentation of the primary endpoint. Probiotic intervention had a significant effect on stool consistency (p = 0.04). The median time for patients to present with Bristol scale stools of Type 6 or greater was 14 days for patients receiving the probiotic drink vs. 10 days for those receiving placebo. Conclusion: Nutritional intervention with the probiotic drink containing L. casei DN-114 001 does not reduce the incidence of radiation-induced diarrhea as defined by a Common Toxicity Criteria Grade 2 or greater. However, it had a significant effect on stool consistency as measured by the Bristol scale

  2. Estudo do processo de incorporação de lactobacillus casei LC-01 em yacon (Smallanthus sonchifolius) desidratado em flocos

    OpenAIRE

    Leone, Roberta de Souza

    2014-01-01

    Resumo: A proposta deste trabalho foi estudar as condições para adicionar Lactobacillus casei (bactéria probiótica) no yacon desidratado, de modo que as bactérias permaneçam viáveis no alimento, por 56 dias de armazenamento, à temperatura ambiente. Para garantir que os prebióticos (inulina e FOS) não fossem degradados pelo processamento, estudou-se o comportamento térmico do yacon, utilizando as técnicas termoanalíticas calorimetria exploratória diferencial (DSC) e termogravimetria (TG), cujo...

  3. The Effect of Lactobacillus casei 32G on the Mouse Cecum Microbiota and Innate Immune Response Is Dose and Time Dependent.

    Directory of Open Access Journals (Sweden)

    Busra Aktas

    Full Text Available Lactobacilli have been associated with a variety of immunomodulatory effects and some of these effects have been related to changes in gastrointestinal microbiota. However, the relationship between probiotic dose, time since probiotic consumption, changes in the microbiota, and immune system requires further investigation. The objective of this study was to determine if the effect of Lactobacillus casei 32G on the murine gastrointestinal microbiota and immune function are dose and time dependent. Mice were fed L. casei 32G at doses of 106, 107, or 108 CFU/day/mouse for seven days and were sacrificed 0.5h, 3.5h, 12h, or 24h after the last administration. The ileum tissue and the cecal content were collected for immune profiling by qPCR and microbiota analysis, respectively. The time required for L. casei 32G to reach the cecum was monitored by qPCR and the 32G bolus reaches the cecum 3.5h after the last administration. L. casei 32G altered the cecal microbiota with the predominance of Lachnospiraceae IS, and Oscillospira decreasing significantly (p < 0.05 in the mice receiving 108 CFU/mouse 32G relative to the control mice, while a significant (p < 0.05 increase was observed in the prevalence of lactobacilli. The lactobacilli that increased were determined to be a commensal lactobacilli. Interestingly, no significant difference in the overall microbiota composition, regardless of 32G doses, was observed at the 12h time point. A likely explanation for this observation is the level of feed derived-nutrients resulting from the 12h light/dark cycle. 32G results in consistent increases in Clec2h expression and reductions in TLR-2, alpha-defensins, and lysozyme. Changes in expression of these components of the innate immune system are one possible explanation for the observed changes in the cecal microbiota. Additionally, 32G administration was observed to alter the expression of cytokines (IL-10rb and TNF-α in a manner consistent with an anti

  4. Host immunity in the protective response to nasal immunization with a pneumococcal antigen associated to live and heat-killed Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Vintiñi Elisa O

    2011-08-01

    Full Text Available Abstract Background At present, available pneumococcal vaccines have failed to eradicate infections caused by S. pneumoniae. Search for effective vaccine continues and some serotype independent pneumococcal proteins are considered as candidates for the design of new vaccines, especially a mucosal vaccine, since pneumococci enter the body through mucosal surfaces. Selection of the appropriate adjuvant is important for mucosal vaccines, and lactic acid bacteria (LAB with immunostimulant properties are promissory candidates. In this work, we assessed the adjuvant effect of a probiotic strain, Lactobacillus casei (L. casei, when nasally administered with a pneumococcal antigen (pneumococcal protective protein A: PppA for the prevention of pneumococcal infection. Adjuvanticity of both live (LcV and heat-killed (LcM was evaluated and humoral and cellular antigen-specific immune response was assessed in mucosal and systemic compartments. The potential mechanisms induced by nasal immunization were discussed. Results Nasal immunization of young mice with PppA+LcV and PppA+LcM induced anti-PppA IgA and IgG antibodies in mucosal and systemic compartments and levels of these specific antibodies remained high even at day 45 after the 3rd Immunization (3rd I. These results were correlated with IL-4 induction by the mixture of antigen plus LcV and LcM. Also, PppA+Lc (V and M induced stimulation of Th1 and Th17 cells involved in the defence against pneumococci. The protection against pneumococcal respiratory challenge at day 30 after the 3rd I showed that PppA+LcV and PppA+LcM immunizations significantly reduced pathogen counts in nasal lavages while prventing their passage into lung and blood. Survival of mice immunized with the co-application of PppA plus LcV and LcM was significantly higher than in mice immunized with PppA alone and control mice when intraperitoneal challenge was performed. No significant differences between the treatments involving LcV and

  5. Bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of Bikalga, an alkaline fermented food

    DEFF Research Database (Denmark)

    Compaor, C.S.; Nielsen, D.S.; Sawadogo-Lingani, H.;

    2013-01-01

    and fungi. Antimicrobial activity against Bacillus cereus was produced in H. sabdariffa seed-based medium. PCR results revealed that the isolates have potential for the lipopeptides iturin, fengycin, surfactin, the polyketides difficidin, macrolactin, bacillaene and the dipeptide bacilysin production. Ultra......-highperformance liquid chromatography-time of flight mass spectrometry analysis of antimicrobial substance produced in BHI broth allowed identification of iturin, fengycin and surfactin. Conclusions: The Bacillus amyloliquefaciens ssp. plantarum exhibited broadspectrum antifungal and antibacterial properties....... They produced several lipopeptide antibiotics and showed good potential for biological control of Bikalga. Significance and Impact of the Study: Pathogenic bacteria often occur in spontaneous food fermentations. This is the first report to identify indigenous B. amyloliquefaciens ssp. plantarum strains...

  6. Generation and characterization of Brassica rapa ssp. pekinensis – B. oleracea var. capitata monosomic and disomic alien addition lines

    Indian Academy of Sciences (India)

    Ai xia Gu; Shu Xing Shen; Yan Hua Wang; Jian Jun Zhao; Shu Xin Xuan; Xue Ping Chen; Xiao Feng Li; Shuang Xia Luo; Yu Jing Zhao

    2015-09-01

    Five monosomic alien addition lines (MAALs) of Brassica rapa ssp. pekinensis – B. oleracea var. capitata were obtained by hybridization and backcrossing between B. rapa ssp. pekinensis (female parent) and B. oleracea var. capitata. The alien linkage groups were identified using 42 B. oleracea var. capitata linkage group-specific markers as B. oleracea linkage groups C2, C3, C6, C7 and C8. Based on the chromosomal karyotype of root tip cells, these five MAALs added individual chromosomes from B. oleracea var. capitata: chr 1 (the longest), chr 2 or 3, chr 5 (small locus of 25S rDNA), chr 7 (satellite-carrying) and chr 9 (the shortest). Five disomic alien addition lines were then generated by selfing their corresponding MAALs.

  7. Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor.

    Science.gov (United States)

    Hanyu, Yuichiro; Imai, Kumiko K; Kawasaki, Yosuke; Nakamura, Takahiro; Nakaseko, Yukinobu; Nagao, Koji; Kokubu, Aya; Ebe, Masahiro; Fujisawa, Asuka; Hayashi, Takeshi; Obuse, Chikashi; Yanagida, Mitsuhiro

    2009-05-01

    Calcium/calmodulin-dependent protein kinase (CaMK) is required for diverse cellular functions, and similar kinases exist in fungi. Although mammalian CaMK kinase (CaMKK) activates CaMK and also evolutionarily-conserved AMP-activated protein kinase (AMPK), CaMKK is yet to be established in yeast. We here report that the fission yeast Schizosaccharomyces pombe Ssp1 kinase, which controls G2/M transition and response to stress, is the putative CaMKK. Ssp1 has a CaM binding domain (CBD) and associates with 14-3-3 proteins as mammalian CaMKK does. Temperature-sensitive ssp1 mutants isolated are defective in the tolerance to limited glucose, and this tolerance requires the conserved stretch present between the kinase domain and CBD. Sds23, multi-copy suppressor for mutants defective in type 1 phosphatase and APC/cyclosome, also suppresses the ssp1 phenotype, and is required for the tolerance to limited glucose. We demonstrate that Sds23 binds to type 2A protein phosphatases (PP2A) and PP2A-related phosphatase Ppe1, and that Sds23 inhibits Ppe1 phosphatase activity. Ssp1 and Ppe1 thus seem to antagonize in utilizing limited glucose. We also show that Ppk9 and Ssp2 are the catalytic subunits of AMPK and AMPK-related kinases, respectively, which bind to common beta-(Amk2) and gamma-(Cbs2) subunits.

  8. The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line

    Directory of Open Access Journals (Sweden)

    Riki M

    2013-02-01

    Full Text Available Background: The aim of this study was to evaluate the effect of extracted cell walls from Lactobacillus casei and Lactobacillus paracasei as probiotic bacteria (isolated from common carp intestine on K562 and the role of cell concentration on the results of MTT [3-(4,5-Dimethylthiazol-2-yl2,5- Diphenyl tetrazolium Bromide] test.Methods: For this purpose, bacteria were cultured in specific medium (MRS broth at anaerobic condition for 24-48 hour. After incubation period culture medium was centri-fuged, then the cells were washed twice with PBS buffer to remove additional medium. Finally, collected bacterial cell disrupted by Sonication and cell walls were separated from other components by centrifugation. After that, different concentrations of cell walls (500, 1000, 2000 and 4000 µg/ml were prepared in RPMI medium for each bacteria, separately. Then anticancer properties of the cell walls were determined in vitro at 12, 24, 48 and 72 h, also the effect of K562 concentration was assayed with MTT technique.Results: The results showed extracted cell wall from both probiotic statistically (P=0.098 have anti turmeric properties in K562 and their properties will arise in relation with concentration. As well as, we found that the number of cell had not any affect on the result of MTT assay.Conclusion: We conclude that the cytotoxicity property of extracted cell wall is related in the type of bacteria, but this anticancer property would warrant further study on the clinical application of extracted cell wall.

  9. Rhizospheric Bacterial Strain Brevibacterium casei MH8a Colonizes Plant Tissues and Enhances Cd, Zn, Cu Phytoextraction by White Mustard.

    Science.gov (United States)

    Płociniczak, Tomasz; Sinkkonen, Aki; Romantschuk, Martin; Sułowicz, Sławomir; Piotrowska-Seget, Zofia

    2016-01-01

    Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants. The aim of this study was to determine whether the multiple-tolerant bacterium, Brevibacterium casei MH8a isolated from the heavy metal-contaminated rhizosphere soil of Sinapis alba L., is able to promote plant growth and enhance Cd, Zn, and Cu uptake by white mustard under laboratory conditions. Additionally, the ability of the rifampicin-resistant spontaneous mutant of MH8a to colonize plant tissues and its mechanisms of plant growth promotion were also examined. In order to assess the ecological consequences of bioaugmentation on autochthonous bacteria, the phospholipid fatty acid (PLFA) analysis was used. The MH8a strain exhibited the ability to produce ammonia, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and HCN but was not able to solubilize inorganic phosphate and produce siderophores. Introduction of MH8a into soil significantly increased S. alba biomass and the accumulation of Cd (208%), Zn (86%), and Cu (39%) in plant shoots in comparison with those grown in non-inoculated soil. Introduced into the soil, MH8a was able to enter the plant and was found in the roots and leaves of inoculated plants thus indicating its endophytic features. PLFA analysis revealed that the MH8a that was introduced into soil had a temporary influence on the structure of the autochthonous bacterial communities. The plant growth-promoting features of the MH8a strain and its ability to enhance the metal uptake by white mustard and its long-term survival in soil as well as its temporary impact on autochthonous microorganisms make the strain a suitable candidate for the promotion of plant growth and the efficiency of phytoextraction. PMID:26909087

  10. Rhizospheric bacterial strain Brevibacterium casei MH8a colonizes plant tissues and enhances Cd, Zn, Cu phytoextraction by white mustard

    Directory of Open Access Journals (Sweden)

    Tomasz ePłociniczak

    2016-02-01

    Full Text Available Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants.The aim of this study was to determine whether the multiple-tolerant bacterium, Brevibacterium casei MH8a isolated from the heavy metal-contaminated rhizosphere soil of Sinapis alba L., is able to promote plant growth and enhance Cd, Zn and Cu uptake by white mustard under laboratory conditions. Additionally, the ability of the rifampicin-resistant spontaneous mutant of MH8a to colonize plant tissues and its mechanisms of plant growth promotion were also examined. In order to assess the ecological consequences of bioaugmentation on autochthonous bacteria, the phospholipid fatty acid (PLFA analysis was used. The MH8a strain exhibited the ability to produce ammonia, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and HCN but was not able to solubilize inorganic phosphate and produce siderophores. Introduction of MH8a into soil significantly increased S. alba biomass and the accumulation of Cd (208%, Zn (86% and Cu (39% in plant shoots in comparison with those grown in non-inoculated soil. Introduced into the soil, MH8a was able to enter the plant and was found in the roots and leaves of inoculated plants thus indicating its endophytic features. PLFA analysis revealed that the MH8a that was introduced into soil had a temporary influence on the structure of the autochthonous bacterial communities. The plant growth-promoting features of the MH8a strain and its ability to enhance the metal uptake by white mustard and its long-term survival in soil as well as its temporary impact on autochthonous microorganisms make the strain a suitable candidate for the promotion of plant growth and the efficiency of phytoextraction.

  11. Sorbitol production from lactose by engineered Lactobacillus casei deficient in sorbitol transport system and mannitol-1-phosphate dehydrogenase.

    Science.gov (United States)

    De Boeck, Reinout; Sarmiento-Rubiano, Luz Adriana; Nadal, Inmaculada; Monedero, Vicente; Pérez-Martínez, Gaspar; Yebra, María J

    2010-02-01

    Sorbitol is a sugar alcohol largely used in the food industry as a low-calorie sweetener. We have previously described a sorbitol-producing Lactobacillus casei (strain BL232) in which the gutF gene, encoding a sorbitol-6-phosphate dehydrogenase, was expressed from the lactose operon. Here, a complete deletion of the ldh1 gene, encoding the main L-lactate dehydrogenase, was performed in strain BL232. In a resting cell system with glucose, the new strain, named BL251, accumulated sorbitol in the medium that was rapidly metabolized after glucose exhaustion. Reutilization of produced sorbitol was prevented by deleting the gutB gene of the phosphoenolpyruvate: sorbitol phosphotransferase system (PTS(Gut)) in BL251. These results showed that the PTS(Gut) did not mediate sorbitol excretion from the cells, but it was responsible for uptake and reutilization of the synthesized sorbitol. A further improvement in sorbitol production was achieved by inactivation of the mtlD gene, encoding a mannitol-1-phosphate dehydrogenase. The new strain BL300 (lac::gutF Deltaldh1 DeltagutB mtlD) showed an increase in sorbitol production whereas no mannitol synthesis was detected, avoiding thus a polyol mixture. This strain was able to convert lactose, the main sugar from milk, into sorbitol, either using a resting cell system or in growing cells under pH control. A conversion rate of 9.4% of lactose into sorbitol was obtained using an optimized fed-batch system and whey permeate, a waste product of the dairy industry, as substrate.

  12. Overcoming interspecific incompatibility in the cross Brassica campestris ssp. japonica x Brassica oleracea var. botrytis using irradiated mentor pollen page

    International Nuclear Information System (INIS)

    The cross B. campestris ssp. japonica x B. oleracea var. botrytis fails due to incompatibility barrier at the stigma. To realize this cross, irradiated compatible pollen (mentor pollen) was used before the incompatible pollination. The presence of mentor pollen stimulated the incompatible pollen to germinate and effect fertilization and seed set. One hybrid was thus obtained. Most of the seeds were inviable. Of the 5 plants raised one was a hybrid and 4 resembled the female parent. 1 tab., 7 refs

  13. Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients

    OpenAIRE

    Parasannanavar, Devraj J.; Anjali Rajadhyaksha; Kanjaksha Ghosh

    2013-01-01

    Background. Microlymphocytotoxicity (MLCT) and flowcytometry (FC) are the conventional serological methods to detect HLA-B* 27. Due to some disadvantages in these methods, most of the HLA laboratories have now switched over to molecular methods. Molecular techniques based on commercial kits are expensive; as such many laboratories with limited funds in developing countries cannot afford these techniques. Aims. Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for...

  14. Conservation of nuclear SSR loci reveals high affinity of Quercus infectoria ssp. veneris A. Kern (Fagaceae) to section Robur

    OpenAIRE

    Neophytou, Charalambos; Dounavi, Aikaterini; Aravanopoulos, Filippos A.

    2013-01-01

    Conservation of 16 nuclear microsatellite loci, originally developed for Quercus macrocarpa (section Albae), Q. petraea, Q. robur (section Robur) and Q. myrsinifolia, (subgenus Cyclobalanopsis) was tested in a Q. infectoria ssp. veneris population from Cyprus. All loci could be amplified successfully and displayed allele size and diversity patterns that match those of oak species belonging to the section Robur. At least in one case, limited amplification and high levels of homozygosity suppor...

  15. 8th International Conference on Solid State Physics (SSP 2004), Workshop “Mössbauer Spectroscopy of Locally Heterogeneous Systems”

    CERN Document Server

    Kadyrzhanov, K. K; SSP 2004

    2006-01-01

    This volume contains papers presented at the 8th International Conference on Solid State Physics (SSP 2004), Workshop "Mössbauer Spectroscopy of Locally Heterogeneous Systems", held in Almaty, Kazakhstan, 23–26 August 2004. It should be of interest to researchers and PhD students working or interested in recent results in the locally inhomogeneous system investigations by Mössbauer Spectroscopy and the new concepts of data evaluation of complex Mössbauer spectra.

  16. Differences between the rhizosphere microbiome of Beta vulgaris ssp. maritima - ancestor of all beet crops - and modern sugar beets

    OpenAIRE

    Christin eZachow; Henry eMueller; Ralf eTilcher; Gabriele eBerg

    2014-01-01

    The structure and function of the plant microbiome is driven by plant species and prevailing environmental conditions. Effectuated by breeding efforts, modern crops diverge genetically and phenotypically from their wild relatives but little is known about consequences for the associated microbiota. Therefore, we studied bacterial rhizosphere communities associated with the wild beet B. vulgaris ssp. maritima grown in their natural habitat soil from coastal drift lines (CS) and modern sugar be...

  17. Computer-assisted diagnostic system for neurodegenerative dementia using brain SPECT and 3D-SSP

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, Kazunari; Kanda, Tomonori; Uemura, Takafumi; Miyamoto, Naokazu; Yoshikawa, Toshiki [Hyogo Brain and Heart Center, Department of Radiology and Nuclear Medicine, Himeji, Hyogo (Japan); Shimada, Kenichi; Ohkawa, Shingo [Hyogo Brain and Heart Center, Institute for Aging Brain and Cognitive Disorders, Himeji, Hyogo (Japan); Minoshima, Satoshi [University of Washington, Radiology and Bioengineering, Department of Radiology, Seattle, WA (United States)

    2009-05-15

    To develop a computer-assisted automated diagnostic system to distinguish among Alzheimer disease (AD), dementia with Lewy bodies (DLB), and other degenerative disorders in patients with mild dementia. Single photon emission computed tomography (SPECT) images with injection of N-Isopropyl-p-[{sup 123}I]iodoamphetamine (IMP) were obtained from patients with mild degenerative dementia. First, datasets from 20 patients mild AD, 15 patients with dementia with DLB, and 17 healthy controls were used to develop an automated diagnosing system based on three-dimensional stereotactic surface projections (3D-SSP). AD- and DLB-specific regional templates were created using 3D-SSP, and critical Z scores in the templates were established. Datasets from 50 AD patients, 8 DLB patients, and 10 patients with non-AD/DLB type degenerative dementia (5 with frontotemporal dementia and 5 with progressive supranuclear palsy) were then used to test the diagnostic accuracy of the optimized automated system in comparison to the diagnostic interpretation of conventional IMP-SPECT images. These comparisons were performed to differentiate AD and DLB from non-AD/DLB and to distinguish AD from DLB. A receiver operating characteristic (ROC) analysis was performed. The area under the ROC curve (Az) and the accuracy of the automated diagnosis system were 0.89 and 82%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 65%, respectively, for AD vs. DLB patients. The mean Az and the accuracy of the visual inspection were 0.84 and 77%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 65%, respectively, for AD vs. DLB patients. The mean Az and the accuracy of the combination of visual inspection and this system were 0.96 and 91%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 66%, respectively, for AD vs. DLB patients. The system developed in the present study achieved as good discrimination of AD, DLB, and other degenerative disorders in patients with mild

  18. Characterizing the Syphilis-Causing Treponema pallidum ssp. pallidum Proteome Using Complementary Mass Spectrometry

    Science.gov (United States)

    Houston, Simon; Lithgow, Karen V.; Meehan, Conor J.; Strouhal, Michal; Šmajs, David; Cameron, Caroline E.; Van Ostade, Xaveer; Kenyon, Chris R.; Van Raemdonck, Geert A.

    2016-01-01

    Background The spirochete bacterium Treponema pallidum ssp. pallidum is the etiological agent of syphilis, a chronic multistage disease. Little is known about the global T. pallidum proteome, therefore mass spectrometry studies are needed to bring insights into pathogenicity and protein expression profiles during infection. Methodology/Principal Findings To better understand the T. pallidum proteome profile during infection, we studied T. pallidum ssp. pallidum DAL-1 strain bacteria isolated from rabbits using complementary mass spectrometry techniques, including multidimensional peptide separation and protein identification via matrix-assisted laser desorption ionization-time of flight (MALDI-TOF/TOF) and electrospray ionization (ESI-LTQ-Orbitrap) tandem mass spectrometry. A total of 6033 peptides were detected, corresponding to 557 unique T. pallidum proteins at a high level of confidence, representing 54% of the predicted proteome. A previous gel-based T. pallidum MS proteome study detected 58 of these proteins. One hundred fourteen of the detected proteins were previously annotated as hypothetical or uncharacterized proteins; this is the first account of 106 of these proteins at the protein level. Detected proteins were characterized according to their predicted biological function and localization; half were allocated into a wide range of functional categories. Proteins annotated as potential membrane proteins and proteins with unclear functional annotations were subjected to an additional bioinformatics pipeline analysis to facilitate further characterization. A total of 116 potential membrane proteins were identified, of which 16 have evidence supporting outer membrane localization. We found 8/12 proteins related to the paralogous tpr gene family: TprB, TprC/D, TprE, TprG, TprH, TprI and TprJ. Protein abundance was semi-quantified using label-free spectral counting methods. A low correlation (r = 0.26) was found between previous microarray signal data and

  19. Engineering and Safety Partnership Enhances Safety of the Space Shuttle Program (SSP)

    Science.gov (United States)

    Duarte, Alberto

    2007-01-01

    Project Management must use the risk assessment documents (RADs) as tools to support their decision making process. Therefore, these documents have to be initiated, developed, and evolved parallel to the life of the project. Technical preparation and safety compliance of these documents require a great deal of resources. Updating these documents after-the-fact not only requires substantial increase in resources - Project Cost -, but this task is also not useful and perhaps an unnecessary expense. Hazard Reports (HRs), Failure Modes and Effects Analysis (FMEAs), Critical Item Lists (CILs), Risk Management process are, among others, within this category. A positive action resulting from a strong partnership between interested parties is one way to get these documents and related processes and requirements, released and updated in useful time. The Space Shuttle Program (SSP) at the Marshall Space Flight Center has implemented a process which is having positive results and gaining acceptance within the Agency. A hybrid Panel, with equal interest and responsibilities for the two larger organizations, Safety and Engineering, is the focal point of this process. Called the Marshall Safety and Engineering Review Panel (MSERP), its charter (Space Shuttle Program Directive 110 F, April 15, 2005), and its Operating Control Plan emphasizes the technical and safety responsibilities over the program risk documents: HRs; FMEA/CILs; Engineering Changes; anomalies/problem resolutions and corrective action implementations, and trend analysis. The MSERP has undertaken its responsibilities with objectivity, assertiveness, dedication, has operated with focus, and has shown significant results and promising perspectives. The MSERP has been deeply involved in propulsion systems and integration, real time technical issues and other relevant reviews, since its conception. These activities have transformed the propulsion MSERP in a truly participative and value added panel, making a

  20. Computer-assisted diagnostic system for neurodegenerative dementia using brain SPECT and 3D-SSP

    International Nuclear Information System (INIS)

    To develop a computer-assisted automated diagnostic system to distinguish among Alzheimer disease (AD), dementia with Lewy bodies (DLB), and other degenerative disorders in patients with mild dementia. Single photon emission computed tomography (SPECT) images with injection of N-Isopropyl-p-[123I]iodoamphetamine (IMP) were obtained from patients with mild degenerative dementia. First, datasets from 20 patients mild AD, 15 patients with dementia with DLB, and 17 healthy controls were used to develop an automated diagnosing system based on three-dimensional stereotactic surface projections (3D-SSP). AD- and DLB-specific regional templates were created using 3D-SSP, and critical Z scores in the templates were established. Datasets from 50 AD patients, 8 DLB patients, and 10 patients with non-AD/DLB type degenerative dementia (5 with frontotemporal dementia and 5 with progressive supranuclear palsy) were then used to test the diagnostic accuracy of the optimized automated system in comparison to the diagnostic interpretation of conventional IMP-SPECT images. These comparisons were performed to differentiate AD and DLB from non-AD/DLB and to distinguish AD from DLB. A receiver operating characteristic (ROC) analysis was performed. The area under the ROC curve (Az) and the accuracy of the automated diagnosis system were 0.89 and 82%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 65%, respectively, for AD vs. DLB patients. The mean Az and the accuracy of the visual inspection were 0.84 and 77%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 65%, respectively, for AD vs. DLB patients. The mean Az and the accuracy of the combination of visual inspection and this system were 0.96 and 91%, respectively, for AD/DLB vs. non-AD/DLB patients, and 0.70 and 66%, respectively, for AD vs. DLB patients. The system developed in the present study achieved as good discrimination of AD, DLB, and other degenerative disorders in patients with mild dementia

  1. Two Novel Alliin Lyase (Alliinase Genes from Twisted-Leaf Garlic (Allium obliquum and Mountain Garlic (Allium senescens ssp. montanum

    Directory of Open Access Journals (Sweden)

    Nicolae DRAGOŞ

    2011-11-01

    Full Text Available Alliinase (Alliin lyase EC 4.4.1.4, a pyridoxal phosphate-dependent lyase, represents one of the major protein components of Allium species. The enzyme is a homodimeric glycoprotein and catalyzes the synthesis of allicin (diallyl thiosulfinate, a biologically active compound, pyruvate, and ammonia starting from the specific non-protein sulfur-containing amino acid alliin ((+S-allyl-L-cysteine sulfoxide. Using newly developed specific primers two new alliinase genes from Allium obliquum and Allium senescens ssp. montanum were amplified and sequenced, as well as their homologs, from Allium fistulosum and Allium schoenoprasum. The G+C content of the alliinase region ranges between that of other dicot plants and that reported in monocot cereal plants, in all four species. Investigations of gene expression revealed a significantly higher enzyme expression level in bulbs than in leaves in all four taxa. The deduced alliinase sequences displayed a high variability among different species, since the lowest sequence similarity was found to be 55.5% between Allium senescens ssp. montanum and Allium cepa, while the highest similarity is 77.5%, between Allium senescens ssp. montanum and Allium fistulosum. Leucine is the most common amino acid in all four alliinases, while cysteine is also more frequent than in other enzymes, suggesting a high stability of the molecules due to the possible disulfide bonds.

  2. Cu,Cr and As determination in preserved woods (Eucalyptus ssp.) by X-ray fluorescence spectrometries

    International Nuclear Information System (INIS)

    Brazil produces around 2.2 millions of cubic meters of treated wood to meet the annual demand of railway, electric, rural and construction sectors. The most used wood species are eucalyptus (Eucalyptus ssp.) and pine (Pinus ssp.).The treated woods used for poles, sleepers, fence posts and plywoods should be according to Brazilian norms requirements. The most usual wood preservative products used in Brazil are CCA (chromated copper arsenate) and CCB (copper chromium and boron salt). The analytical methods, such as flame atomic absorption spectrometry (FAAS), plasma inductively coupled optical emission spectrometry (ICPOES) and X-ray fluorescence spectrometry (XRFS) have been used for the analytical control of those treatment processes. In this work, the eucalyptus trees (Eucalyptus ssp) samples was obtained from Minas Gerais State, Brazil, cut plantation areas. Under pressure, eucalyptus wood samples were submitted to different concentration of CCA solution reaching 3.9, 6.7, 9.1, 12.4 and 14.0 kg of CCA by m-³ sapwood retentions. Samples in cylinders and sawdust forms were obtained from treated wood samples. Copper, chromium and arsenic determination was performed using the energy dispersive X-ray fluorescence spectrometry (EDXRFS), portable X-ray fluorescence spectrometry (PXRFS), flame atomic absorption spectrometry (FAAS) and instrumental neutron activation analysis. In this work, the method of analysis, sensitivity, precision and accuracy performances of the related techniques were outlined. (author)

  3. Influence of different strains of Agrobacterium rhizogenes on induction of hairy roots and lignan production in Linum tauricum ssp. tauricum

    Directory of Open Access Journals (Sweden)

    Iliana Ionkova

    2009-01-01

    Full Text Available Hairy root cultures were induced from leaf explants of Linum tauricum ssp. Tauricum by infection with Agrobacterium rhizogenes. Different bacterial strains of Agrobacterium rhizogenes - TR 105 and ATCC 15834 were evaluated for induction of transformed hairy roots in Linum tauricum ssp. Tauricum. These different strains varied in their virulence for induction of hairy roots in this species. Acetosyringon in cultivation medium was used to increase of frequency of hairy root induction. Growth kinetics of transgenic roots indicated a similar pattern of growth, with maximum growth occurring between 17 and 20 days. The transformed nature of tissue was confirmed by the production of opines. The lignin production of different clones was found to be growth-related. The cultures produced to 2.6% of the lignin 4′-demethyl-6-methoxypodophylotoxin (4′-DM-MPTOX and to 3.5% of the lignin 6-methoxypodophyllotoxin (6MPTOX on a dry weight basis, which was 10 to 12 times higher than in Linum tauricum ssp. Tauricum cell suspensions. Transformed cultures showed significant differences in lignin content. The highest amount of 4′-DM-MPTOX and MPTOX was found in transformed line induced by strain ATCC 15834. Rapidly growing root lines were selected to increase the efficiency of he production of lignans.

  4. Accumulation of biologically active furanocoumarins in agitated cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams.

    Science.gov (United States)

    Ekiert, H; Czygan, F-Ch

    2005-08-01

    This study was designed to investigate the dynamics of accumulation of linear furanocoumarins (psoralen, bergapten, xanthotoxin, isopimpinellin, imperatorin) and their biogenetic precursor, umbelliferone, in agitated cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams during 6-week growth cycles. The metabolites under study were almost exclusively accumulated in the cultured biomass. The total content of all metabolites increased 4.8- and 2.0-fold, in R. graveolens and R. graveolens ssp. divaricata cultures, respectively. Xanthotoxin and bergapten, which are the most important therapeutic compounds, were the dominating metabolites in cultures of both plants. The maximum content of xanthotoxin (25.0 mg/100 g dry wt.) and bergapten (18.4 mg/100 g dry wt) and the maximum content of all metabolites (64.0 mg/100 g dry wt) in R. graveolens ssp. divaricata callus obtained on the 35th culture day were relatively low. However, maximum contents of xanthotoxin (136.8 mg/100 g dry wt), bergapten (210.4 mg/100 g dry wt.) and isopimpinellin (96.7 mg/100 g dry wt), and total content of all metabolites in R. graveolens shoots (520.8 mg/100 g dry wt) obtained on the 42nd culture day are interesting from a practical point of view.

  5. Lactobacillus rhamnosus GR-1 Limits Escherichia coli-Induced Inflammatory Responses via Attenuating MyD88-Dependent and MyD88-Independent Pathway Activation in Bovine Endometrial Epithelial Cells.

    Science.gov (United States)

    Liu, Mingchao; Wu, Qiong; Wang, Mengling; Fu, Yunhe; Wang, Jiufeng

    2016-08-01

    Intrauterine Escherichia coli infection after calving reduces fertility and causes major economic losses in the dairy industry. We investigated the protective effect of the probiotic Lactobacillus rhamnosus GR-1 on E. coli-induced cell damage and inflammation in primary bovine endometrial epithelial cells (BEECs). L. rhamnosus GR-1 reduced ultrastructure alterations and the percentage of BEECs apoptosis after E. coli challenge. Increased messenger RNA (mRNA) expression of immune response indicators, including pattern recognition receptors (toll-like receptor [TLR]2, TLR4, nucleotide-binding oligomerization domain [NOD]1, and NOD2), inflammasome proteins (NOD-like receptor family member pyrin domain-containing protein 3, apoptosis-associated speck-like protein, and caspase-1), TLR4 downstream adaptor molecules (myeloid differentiation antigen 88 [MyD88], toll-like receptor adaptor molecule 2 [TICAM2]), nuclear transcription factor kB (NF-kB), and the inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-18, and interferon (IFN)-β, was observed following E. coli challenge. However, these increases were attenuated by L. rhamnosus GR-1 pretreatment. Our data indicate that L. rhamnosus GR-1 ameliorates the E. coli-induced disruption of cellular ultrastructure, subsequently reducing the percentage of BEECs apoptosis and limiting inflammatory responses, partly via attenuation of MyD88-dependent and MyD88-independent pathway activation. Certain probiotics could potentially prevent postpartum uterine diseases in dairy cows, ultimately reducing the use of antibiotics. PMID:27236308

  6. Growth Behavior of Phytopathogen Clavibacter michiganensis ssp. sepedonicus Treated with Selenium Biocomposites of Mushroom Origin

    Directory of Open Access Journals (Sweden)

    A.I. Perfileva

    2016-02-01

    Full Text Available The results of studying the effect of biologically obtained selenium nanocomposites on the bacterium Clavibacter michiganensis ssp. sepedonicus (Cms are presented. Cms is a Gram-positive bacterium, which causes one of the most dangerous potato diseases, ring rot. The effective alongside ecologically safe methods for combating Cms are lacking. As the agents feasible for use in this purpose, we examined the selenium nanocomposites obtained from the macrobasidiomycetes' submerged cultures. For exploring the bionanocomposites effect on Cms, the methods of agar well diffusion, the suspension turbidity measurement, and the colony-forming units count were applied. The results showed that all the nanocomposites under study lowered the bacterial suspension's absorption values compared to the reference specimen, that testified to the observation of bacteriostatic effect of the agents tested. The suppression action of nanocompostes was elucidated by means of both agar well diffusion assay and colony-forming units count. Thus, the results obtained demonstrate the occurrence of bacteriostatic and bactericidal effects of the substances under study, and favor the supposition on advisability of further research into the selenium nanocomposites as the agents for agricultural recovery from the bacterial pathogens.

  7. Comparative Transcriptome Analysis Reveals Heat-Responsive Genes in Chinese Cabbage (Brassica rapa ssp. chinensis)

    Science.gov (United States)

    Wang, Aihua; Hu, Jihong; Huang, Xingxue; Li, Xia; Zhou, Guolin; Yan, Zhixiang

    2016-01-01

    Chinese cabbage (Brassica rapa ssp. chinensis) is an economically and agriculturally significant vegetable crop and is extensively cultivated throughout the world. Heat stress disturbs cellular homeostasis and causes visible growth inhibition of shoots and roots, severe retardation in growth and development, and even death. However, there are few studies on the transcriptome profiling of heat stress in non-heading Chinese cabbage. In this study, we investigated the transcript profiles of non-heading Chinese cabbage from heat-sensitive and heat-tolerant varieties “GHA” and “XK,” respectively, in response to high temperature using RNA sequencing (RNA seq). Approximately 625 genes were differentially expressed between the two varieties. The responsive genes can be divided into three phases along with the time of heat treatment: response to stimulus, programmed cell death and ribosome biogenesis. Differentially expressed genes (DEGs) were identified in the two varieties, including transcription factors (TFs), kinases/phosphatases, genes related to photosynthesis and effectors of homeostasis. Many TFs were involved in the heat stress response of Chinese cabbage, including NAC069 TF which was up-regulated at all the heat treatment stages. And their expression levels were also validated by quantitative real-time-PCR (qRT-PCR). These candidate genes will provide genetic resources for further improving the heat-tolerant characteristics in non-heading Chinese cabbage. PMID:27443222

  8. Improving green roofs and rail road greening systems using Bacillus subtilis and Lactobacillus ssp.

    Science.gov (United States)

    Grüneberg, H; Oschmann, C; Dunya, S; Ulrichs, C

    2006-01-01

    Aim of the present study was the improvement of existing methods for green roof and rail road greening systems using soil borne bacteria. Bacillus subtilis and Lactobacillus ssp. alone and in combination with vinasse applied to different growing substrates were tested. The substrates were brick chips, textile mats, mineral wool mats, and a commercial available substrate for the Swedish company VegTech. All four substrates were tested along an artificial rail track on the experimental station at Humboldt University Berlin, and partly on an existing rail track in Munich, Germany. Plants selected for the experiments belong to the genus Sedum, which is relatively tolerant to dry conditions. Inoculation of plants with bacteria had no effect on plant growth parameters and on coverage of different mobile bedding systems with Sedum plants. There was no significant difference between the various treatments in Munich. In both experiments, the addition of vinasse alone improved plant growth. Plant growth was significantly different on all substrates, whereas brick chips and the commercial roof soil was the best substrate. Brick chips are a cheap substrate which can be used for rail track greening. The results indicate that the quality of the substrate is the most important factor for remediation and greening of rail tracks and roof tops. The rapid growth of plants can be influenced by the application of vinasse as additional nutrient solution (potash (K) source) or nutrient enriched substrate.

  9. Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays

    Directory of Open Access Journals (Sweden)

    Sodergren Erica

    2008-05-01

    Full Text Available Abstract Background Syphilis spirochete Treponema pallidum ssp. pallidum remains the enigmatic pathogen, since no virulence factors have been identified and the pathogenesis of the disease is poorly understood. Increasing rates of new syphilis cases per year have been observed recently. Results The genome of the SS14 strain was sequenced to high accuracy by an oligonucleotide array strategy requiring hybridization to only three arrays (Comparative Genome Sequencing, CGS. Gaps in the resulting sequence were filled with targeted dideoxy-terminators (DDT sequencing and the sequence was confirmed by whole genome fingerprinting (WGF. When compared to the Nichols strain, 327 single nucleotide substitutions (224 transitions, 103 transversions, 14 deletions, and 18 insertions were found. On the proteome level, the highest frequency of amino acid-altering substitution polymorphisms was in novel genes, while the lowest was in housekeeping genes, as expected by their evolutionary conservation. Evidence was also found for hypervariable regions and multiple regions showing intrastrain heterogeneity in the T. pallidum chromosome. Conclusion The observed genetic changes do not have influence on the ability of Treponema pallidum to cause syphilitic infection, since both SS14 and Nichols are virulent in rabbit. However, this is the first assessment of the degree of variation between the two syphilis pathogens and paves the way for phylogenetic studies of this fascinating organism.

  10. RAPID HLA-DRB1 GENERIC TYPING BY PCR-SSP METHOD

    Institute of Scientific and Technical Information of China (English)

    邱长春; 赵岩; 陶贞寅; 朱席林; 徐作军; 董怡

    1995-01-01

    We report a simple and rapid HLA-DRB1 generic typing metbod,PCR-SSP,which is practical and inexpensive.We use 9 sequence-specific primers and 2 group specific primers to define the HLA-DRB1 specificities DR1,DR2,DR3,DR4,DR5,DR6,DR7,DR8,DR9 and DR10. The HLA DR3,DR5,DR6 and DR8 can be amplified by the two primers of DR3568 are positive.Any individuals can be typed with some exception: the three pairs of phenotype DR3/DR3 and DR3/DR6,DR5/DR5 and DR5/DR6, DR8/DR8 and DR8/DR6 cannot be discriminated from each other.We typed 106 unrelated healthy people from Beijing locations in two weeks.We think this typing method is suitable to replace the error-Prone serologic HLA-DR tests in routine clinical practice,including the prospective typing of cadaveric organ donors.

  11. The essential oil of the protected species: Thymus praecox ssp. penyalarensis

    Directory of Open Access Journals (Sweden)

    José Blanco Salas

    2012-03-01

    Full Text Available The essential oil of a protected Spanish species, Thymus praecox ssp. penyalarensis, was firstly analyzed [Gas Chromatography-Mass Spectrometry (GC-MS] from wild populations during the phenological stages of flowering and fruiting. In flowering, the yield was 1.2%, and the major components were identified as thymol (18.5%, p-cymene (14.6%, carvacrol (11.6%, and γ-terpinene (10.1%. In fruiting, the yield was 0.9% and the major components resulted to be p-cymene (19.0%, carvacrol (16.5%, and borneol (10.5%. An experimental cultivation of the taxa was made also in order to analyze its essential oil as well. The yield was 1.3%, and the main compounds were thymol (19.1%, p-cymene (17.0%, and γ-terpinene (12.3%. Finally, the composition of the essential oil of this plant is discussed in relation to chemotaxonomic and biogeographical aspects.

  12. Anatomical and micromorphological particularities of vegetative organs in endemic Erysimum wittmanii Zaw. ssp. wittmanii

    Directory of Open Access Journals (Sweden)

    Irina Neta GOSTIN

    2009-11-01

    Full Text Available The Erysimum wittmanii ssp. wittmanii is an endemic biennial herbaceous species in South and East Carpathians. In this study the anatomical and micromorphological properties of this specie are investigated. Anatomical properties of root, stem and leaves were underlined. Roots acquire early in ontogenesis the secondary structure. Stems presents collateral vascular bundles both in primary and in secondary structure; cortical small bundles by concentric type, was observed on the cross sections from the top of the stem. The leaves are bifacial and amphystomatic. Scanning electron microscopy investigation regarding upper and lower epidermises of leaves in different developmental stages was carried out. Trichomes occur on both faces of young leaves. The tector hairs are multicelullar, usually three-armed; rarely four-armed or two-armed (especially on the epidermis from the veins. The hairs cell walls are thin and the surface is smooth in the first developmental stages (on very young leaves; on the young and mature leaves the trichomes have thick-walled cells, densely covered by micro-papillae.

  13. Influence of mowing Artemisia tridentata ssp. wyomingensis on winter habitat for wildlife.

    Science.gov (United States)

    Davies, Kirk W; Bates, Jonathan D; Johnson, Dustin D; Nafus, Aleta M

    2009-07-01

    Mowing is commonly implemented to Artemisia tridentata ssp. wyomingensis (Beetle & A. Young) S.L. Welsh (Wyoming big sagebrush) plant communities to improve wildlife habitat, increase forage production for livestock, and create fuel breaks for fire suppression. However, information detailing the influence of mowing on winter habitat for wildlife is lacking. This information is crucial because many wildlife species depended on A. tridentata spp. wyomingensis plant communities for winter habitat and consume significant quantities of Artemisia during this time. Furthermore, information is generally limited describing the recovery of A. tridentata spp. wyomingensis to mowing and the impacts of mowing on stand structure. Stand characteristics and Artemisia leaf tissue crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) concentrations were measured in midwinter on 0-, 2-, 4-, and 6-year-old fall-applied mechanical (mowed at 20 cm height) treatments and compared to adjacent untreated (control) areas. Mowing compared to the control decreased Artemisia cover, density, canopy volume, canopy elliptical area, and height (P plant communities slightly increases the nutritional quality of Artemisia leaves (P winter habitat. Considering the decline in A. tridentata spp. wyomingensis-dominated landscapes, we caution against mowing these communities.

  14. Characterization of a sagebrush (Artemisia tridentata ssp. wyomingensis) die-off on the Handford Site

    International Nuclear Information System (INIS)

    The Hanford Site contains one of the few remaining contiguous areas of shrub-steppe habitat left in Washington State. This habitat is home to many native plant and wildlife species, some of which are threatened with extinction or are unique to the Site. The importance of the Hanford Site increases as other lands surrounding the Site are developed, and these native species and habitats are lost. Stands of Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis) on the Site are a particularly important component of shrub-steppe habitat, because a number of wildlife require big sagebrush for food and cover. Since 1993, researchers and field biologists have made anecdotal observations of dying and declining sagebrush in stands of shrubs near the 100 Areas. This study was initiated to delineate and document the general boundary where sagebrush stands appear to be declining. We mapped the areal extent of the die-off using a global positioning system and found that the central portion of the die-off encompasses 280 hectares. Shrub stand defoliation was estimated to be near or greater than 80% in this area. The remainder of the die-off area exhibits varying mixtures of completely defoliated, partially defoliated, and healthy-looking stands. Declining sagebrush stands comprise a total of 1776 hectares

  15. Immunostimulatory oligonucleotide, CpG-like motif exists in Lactobacillus delbrueckii ssp. bulgaricus NIAI B6.

    Science.gov (United States)

    Kitazawa, Haruki; Watanabe, Hiroshi; Shimosato, Takeshi; Kawai, Yasushi; Itoh, Takatoshi; Saito, Tadao

    2003-08-15

    The present study was conducted to find an immunostimulatory oligonucleotide derived from yogurt starter cultures. The chromosomal DNA was purified from nine strains of Lactobacillus delbrueckii ssp. bulgaricus and six strains of Streptococcus thermophilus. An immunostimulatory ability of the DNA was examined in a proliferation of peyer's patch and splenic B cells. Only the DNA from L. bulgaricus NIAI B6 induced a significant proliferation of both cells. When the DNA was cloned and amplified using PCR, the mitogenic activities to B cells were significantly increased by 13 of 135 DNA clones. Ten homologous nucleotide sequences were found as possible oligonucleotide sequences of mitogens, and were then chemically synthesized (sOL-LB1 to sOL-LB10). One CpG-like motif (sOL-LB7; 5'-CGGCACGCTCACGATTCTTG-3') was identified as an immunostimulatory oligonucleotide, but it did not contain palindromic CpG structure known as a B cell-specific mitogen. The sOL-LB7 substantially bound to B cells and increased the CD69 positive cells in peyer's patch cells. This study demonstrated that L. bulgaricus NIAI B6 was a good candidate of a starter culture for the production of new functional foods, "Bio-Defense Foods".

  16. Isolation and characterization of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus from plants in Bulgaria.

    Science.gov (United States)

    Michaylova, Michaela; Minkova, Svetlana; Kimura, Katsunori; Sasaki, Takashi; Isawa, Kakuhei

    2007-04-01

    One of the traditional ways of preparation of yogurt starter in Bulgaria is placing a branch of a particular plant species into boiled sheep's milk maintained at about 45 degrees C, which is further incubated until a dense coagulum is obtained. To investigate the possible origin of the yogurt starter bacteria, Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) and Streptococcus thermophilus (S. thermophilus), the traditional way of yogurt-starter preparation was followed. Hundreds of plant samples were collected from four regions in Bulgaria and incubated in sterile skim milk. The two target bacteria at low frequencies from the plant samples collected were successfully isolated. Phenotypic and genotypic characteristics of these bacterial isolates revealed that they were identified as L. bulgaricus and S. thermophilus. Twenty isolates of L. bulgaricus and S. thermophilus, respectively, were selected from the isolated strains and further characterized with regard to their performance in yogurt production. Organoleptic and physical properties of yogurt prepared using the isolated strains from plants were not significantly different from those prepared using commercial yogurt-starter strains. It was therefore suggested that L. bulgaricus and S. thermophilus strains widely used for commercial yogurt production could have originated from plants in Bulgaria. To our knowledge, this is the first report on the isolation and characterization of L. bulgaricus and S. thermophilus strains from plants.

  17. Variation in Nectar Volume and Sugar Concentration of Allium ursinum L. ssp. ucrainicum in Three Habitats

    Directory of Open Access Journals (Sweden)

    Ágnes Farkas

    2012-01-01

    Full Text Available Floral nectar volume and concentration of ramson (Allium ursinum L. ssp. ucrainicum were investigated in three different habitats, including two types of sessile oak-hornbeam association on brown forest soil with clay illuviation and a silver lime-flowering ash rock forest association on rendzina. Daily nectar production ranged from 0.1 to 3.8 μL per flower with sugar concentrations of 25 to 50%. Mean nectar volumes and concentrations showed significant differences between freely exposed flowers and covered flowers, which had been isolated from flower visitors 24 h prior to nectar studies. Both the amount and quality of nectar were affected by microclimatic conditions and soil properties and varied between populations at different habitats. In the silver lime-flowering ash rock-forest association mean nectar volumes and concentrations were lower than in a typical sessile oak-hornbeam association on three occasions, the difference being significant in two cases. During full bloom, the date of sampling did not have a profound effect on either nectar volume or concentration.

  18. Leaf water relations of Eucalyptus globulus ssp. globulus and E. nitens: seasonal, drought and species effects.

    Science.gov (United States)

    White, D A; Beadle, C L; Worledge, D

    1996-05-01

    In August 1990, a 2-ha plantation was established in an area where rainfall (about 515 mm year(-1)) was insufficient to meet evaporative demand. On nine occasions between September 1991 and April 1993, pressure-volume curves were constructed for irrigated and rainfed Eucalyptus globulus ssp. globulus Labill. and E. nitens (Deane and Maiden) Maiden trees. During the experiment, rainfed trees experienced six periods when predawn water potential was significantly lower than that of irrigated trees. In early spring of 1991 and 1992, osmotic potentials at full turgor and turgor loss point in the irrigated E. nitens were significantly lower than at other times of the year, probably because of winter hardening. Water stress reduced osmotic potential and increased bulk elastic modulus in E. nitens, whereas the reverse occurred in E. globulus. However, treatment differences with respect to changes in osmotic and elastic properties were commonly overshadowed by interspecific differences. These were most apparent at the end of the sixth period of water stress when osmotic potentials at full and zero turgor were significantly higher and bulk elastic modulus and relative water content at turgor loss point were significantly lower in E. globulus than in E. nitens. We conclude that the drought-tolerance responses of E. globulus make it a more suitable species than E. nitens for establishment on sites where moderate water stress is experienced.

  19. The Discovery of Resistant Sources of Spring Barley, Hordeum vulgare ssp. spontaneum, and Unique Greenbug Biotypes.

    Science.gov (United States)

    Armstrong, J Scott; Mornhinweg, Dolores W; Payton, Mark E; Puterka, Gary J

    2016-02-01

    The genetic sources for host-plant resistance to the greenbug (Schizaphis graminum Rondani) in barley (Hordeum vulgare ssp. spontaneum) are limited in that only two single dominant genes Rsg1 and Rsg2 are available for the complex of greenbug biotypes. We evaluated four new barley lines from the Wild Barley Diversity Collection (WBDC) that previously showed potential for greenbug resistance. Three of those entries, WBDC 53, WBDC 117, WBDC 336, exhibited very dominant sources of resistance to older known biotypes B, C, E, F, H, I, and TX1, which also add to the host-plant differentials used to separate these greenbug biotypes. We also re-evaluated the earlier known set of greenbug biotypes that have been in culture for several years against the known host-plant differentials, and included seven newer greenbug isolates collected from Wyoming to the full complement of small grain differentials. This resulted in the discovery of five new greenbug biotypes, WY10 MC, WY81, WY10 B, WY12 MC, and WY86. Wyoming isolates WY4 A and WY4 B were identical in their phenotypic profile, and should be combined as a single unique greenbug biotype. These barley trials resulted in finding new sources of host-plant resistance, although more research needs to be conducted on what type of resistance was found, and how it can be used. We also document that the Wheatland, Wyoming area serves as a very conducive environment for the development of new greenbug biotypes. PMID:26527793

  20. Chemical composition and nutritional evaluation of the seeds of Acacia tortilis (Forssk.) Hayne ssp. raddiana.

    Science.gov (United States)

    Embaby, Hassan E; Rayan, Ahmed M

    2016-06-01

    Chemical composition and nutritional evaluation as well as physicochemical and functional properties of seed flour of Acacia tortilis (Forssk.) Hayne ssp. raddiana were studied. The results indicated that seeds contained 5.30% moisture, 3.99% ash, 9.19% fat, 14.31% fiber, 27.21% protein and 45.30% carbohydrates. Potassium was the predominant element followed by calcium and then phosphorous. Phytic acid, tannins and trypsin inhibitor as antinutrients were detected. The amino acid profile compared well with FAO/WHO recommended pattern except for cystine/methionine, isoleucine, tyrosine/phenylalanine, lysine and threonine. Also, the first limiting amino acid was lysine. Fatty acid composition showed that linoleic acid was the major fatty acid, followed by palmitic, stearic, oleic and arachidic acids. The seed oil showed absorbance in the ultraviolet ranges, thus it can be used as a broad spectrum UV protectant. For physicochemical and functional properties, acacia seeds flour had excellent water holding index, swelling index, foaming capacity and foam stability. PMID:26830561

  1. Comparative Transcriptome Analysis Reveals Heat-Responsive Genes in Chinese Cabbage (Brassica rapa ssp. chinensis).

    Science.gov (United States)

    Wang, Aihua; Hu, Jihong; Huang, Xingxue; Li, Xia; Zhou, Guolin; Yan, Zhixiang

    2016-01-01

    Chinese cabbage (Brassica rapa ssp. chinensis) is an economically and agriculturally significant vegetable crop and is extensively cultivated throughout the world. Heat stress disturbs cellular homeostasis and causes visible growth inhibition of shoots and roots, severe retardation in growth and development, and even death. However, there are few studies on the transcriptome profiling of heat stress in non-heading Chinese cabbage. In this study, we investigated the transcript profiles of non-heading Chinese cabbage from heat-sensitive and heat-tolerant varieties "GHA" and "XK," respectively, in response to high temperature using RNA sequencing (RNA seq). Approximately 625 genes were differentially expressed between the two varieties. The responsive genes can be divided into three phases along with the time of heat treatment: response to stimulus, programmed cell death and ribosome biogenesis. Differentially expressed genes (DEGs) were identified in the two varieties, including transcription factors (TFs), kinases/phosphatases, genes related to photosynthesis and effectors of homeostasis. Many TFs were involved in the heat stress response of Chinese cabbage, including NAC069 TF which was up-regulated at all the heat treatment stages. And their expression levels were also validated by quantitative real-time-PCR (qRT-PCR). These candidate genes will provide genetic resources for further improving the heat-tolerant characteristics in non-heading Chinese cabbage. PMID:27443222

  2. Full Scale Test of SSP 34m blade, edgewise loading LTT. Data Report 1

    Energy Technology Data Exchange (ETDEWEB)

    Nielsen, Magda; Jensen, Find M.; Nielsen, Per H. (and others)

    2010-01-15

    This report is a part of a research project where a 34m wind turbine blade from SSP-Technology A/S has been tested in edgewise direction (LTT). The applied load is 60% of an unrealistic extreme event, corresponding to 75% of a certificated extreme load. This report describes the background, the test set up, the tests and the results. For this project, a new solution has been used for the load application and the solution for the load application is described in this report as well. The blade has been submitted to thorough examination. More areas have been examined with DIC, both global and local deflections have been measured, and also 378 strain gauge measurements have been performed. Furthermore Acoustic Emission has been used in order to detect damage while testing new load areas. The global deflection is compared with results from a previous test and results from FEM analyses in order to validate the solution as to how the gravity load on the blade was handled. Furthermore, the DIC measurement and the displacement sensors measurements are compared in order to validate the results from the DIC measurements. The report includes the results from the test and a description of the measurement equipment and the data acquisition. (author)

  3. Effects of amendments on the uptake and distribution of DDT in Cucurbita pepo ssp pepo plants

    International Nuclear Information System (INIS)

    The effects of soil amendments on the phytoextraction of ΣDDT (DDT + DDD + DDE) from soil ([ΣDDT] ∼ 1500 ng/g) by a pumpkin variety of Cucurbita pepo ssp pepo were tested and the patterns of ΣDDT storage throughout the plant shoot were examined. The soil amendments did not increase the total amount of ΣDDT extracted into plant shoots, but new information about ΣDDT distribution in the plants was obtained. As observed previously, the ΣDDT concentration in plant leaves (mean 290 ng/g) was significantly lower than in plant stems (mean 2600 ng/g). Further analysis revealed that ΣDDT composition was consistent throughout the plant shoot and that ΣDDT concentration in leaves and stems decreased exponentially as distance from the root increased, which was previously unknown. This new information about the patterns of ΣDDT uptake and translocation within pumpkin plants highlights the need for appropriate plant sampling strategies in future POPs phytoextraction research. - Patterns of ΣDDT storage in a pumpkin plant are elucidated and specific surfactant and mycorrhizal soil amendments did not increase the total amount of ΣDDT phytoextracted into plant shoots.

  4. Optimization Technology of the Production of Lactobacillus casei Yoghurt%干酪乳杆菌发酵酸乳生产工艺优化

    Institute of Scientific and Technical Information of China (English)

    张杰; 郗宏波; 苏贺靖; 王艳萍

    2013-01-01

      以从日本传统酸奶中分离选育出的优质干酪乳杆菌作为试验菌种。通过单因素试验和正交试验确定最佳发酵工艺条件。结果表明:嗜热链球菌、保加利亚乳杆菌、干酪乳杆菌配比为1∶1∶2;接种量为5%;发酵温度37℃;乳固体含量12%为最佳参数组合。%Lactobacillus casei which was separated and identified from the Japanese traditional yoghurt was taken as the test strains. By the single factor experiments and orthogonal tests to determine the best fermentation conditions, the results showed that the best fermentation parameter combination was the ratio of Streptococcus thermophilus:Lactobacillus bulgaricus:Lactobacillus casei of 1∶1∶2, inoculated quantity of 5%, fermentation temperature of 37℃, milk solid content of 12%.

  5. PRODUCTION UNDER CONTROLLED CONDITIONS OF “CASU MARZU” CHEESE: EFFECT OF THE Piophila Casei COLONIZATION ON MICROBIAL AND CHEMICAL COMPOSITION OF THE CHEESES

    Directory of Open Access Journals (Sweden)

    M Coinu

    2013-02-01

    Full Text Available The aim of the present study was to evaluate the effect of the Piophila casei colonization under controlled conditions on “casu marzu”, a typical Sardinian sheep milk cheese. Three batches of two different kind of cheese (PO, holed paste and PC, firm paste were produced in duplicate (test and control. Test cheeses were exposed to the Piophila casei colonization in a conditioned store room, while control cheeses were suitably protected. All the samples were analyzed at 0, 10, 30, 60 and 90 days from the production. The pH and aw, the chemical composition and the microbiological parameters (food safety and process hygiene criteria were determined. The colonization was unhomogeneous between the batches: PO cheeses were more easily colonized and showed more intense proteolysis and lipolysis respect to PC cheeses. The microbiological parameters were strongly affected by the experimental process conditions. The authors discuss about the opportunity of a controlled production of “casu marzu” and the implications to the health of consumers.

  6. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to Lactobacillus casei DG CNCM I-1572 and decreasing potentially pathogenic gastro-intestinal microorganisms (ID 2949, 3061, further assessment) pursuant to Article 13

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006 in the framework of further assessment related to Lactobacillus casei...... DG CNCM I-1572 and decreasing potentially pathogenic gastro-intestinal microorganisms. The food constituent that is the subject of the health claim, Lactobacillus casei DG CNCM I-1572, is sufficiently characterised. The claimed effect, decreasing potentially pathogenic gastro...... and effect relationship has not been established between the consumption of Lactobacillus casei DG CNCM I-1572 and decreasing potentially pathogenic gastro‑intestinal microorganisms....

  7. Plasmid stability and viable count of recombinant Lactobacillus casei during storage period%重组干酪乳杆菌贮存期内质粒稳定性及活菌数的检测

    Institute of Scientific and Technical Information of China (English)

    曹宁; 侯喜林; 谭君; 刘彩霞; 武龙宇; 余丽芸

    2013-01-01

    目的 检测产肠毒素性大肠杆菌(Enterotoxigenic E.coli,ETEC)基因重组干酪乳杆菌(Lactobacillus casei,L.casei)贮存过程中质粒的稳定性及活菌数的变化.方法 将ETEC K88重组于酪乳杆菌(pLA-K88/L.casei)接种于MRS培养基,转种WPC培养基,收集菌液,置4、15、37℃贮存.通过抗生素MRS(+)和MRS(-)平板计数菌落数的方法及PCR法检测重组质粒在4℃贮存1、15、30、60、90和120 d的稳定性;Dot ELISA法检测菌液外源蛋白免疫学活性的变化;平板计数法检测4、15、37℃贮存1、2、3、7、15、30、60、90、120、210和240 d的活菌数.结果 在4℃贮存120 d内,重组质粒稳定性良好,目的基因片段无缺失,外源蛋白活性稳定,乳清蛋白对工程菌的稳定性及外源蛋白的免疫原性均无影响;工程菌在4℃贮存效果最好,存活时间最长,第240 d活菌数仍保持在9×106 cfu/ml以上,15和37℃贮存期仅为90和15d.结论 ETEC基因重组干酪乳杆菌于4℃贮存条件下,质粒及外源基因表达稳定.%Objective To observe the plasmid stability and viable count of recombinant Lactobacillus casei with entero-toxigenic E. coli (ETEC) gene during storage period. Methods Recombinant L. casei pLA-K88/L casei was inoculated to MRS medium then transferred to WPC medium, and the bacterial liquid was collected and stored at 4, 15 and 37 ℃ respectively. The stabilities of recombinant plasmid after storage at 4 ℃ for 1, 15, 30, 60, 90 and 120 d were tested by colony counting on MRS( + ) and MRS(-) plates and PCR. The immune activity of foreign protein was determined by Dot ELISA. The viable counts of recombinant L. casei 1, 2, 3, 7, 15, 30, 60, 90, 120, 210 and 240 d after storage at 4, 15 and 37 ℃ were determined by plate counting. Results The recombinant plasmid showed high stability within 120 d after storage at 4 ℃. No target gene deletion was observed, while the activity of foreign protein was stable. Lactoalburnin showed no

  8. Identification of an HLA-A* 0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein

    Institute of Scientific and Technical Information of China (English)

    Wang B; Yu Y; Wang X; Yang R; Cao X; Chen H; Jiang X; Zhang M; Wan T; Li N; Zhou X; Wu Y; Yang F

    2004-01-01

    A novel coronavirus, severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV), has been identified as the causal agent of SARS. Spike (S) protein is a major structural glycoprotein of the SARS virus and a potential target for SARS-specific cell-mediated immune responses. A panel of S protein-derived peptides was tested for their binding affinity to HLA-A * 0201 molecules. Peptides with high affinity for HLA-A * 0201 were then assessed for their capacity to elicit specific immune responses mediated by cytotoxic T lymphocytes (CTLs) both in vivo, in HLA-A2. 1/Kb transgenic mice, and in vitro, from peripheral blood lymphocytes (PBLs) harvested from healthy HLA-A2.1 + donors. SARS-CoV protein-derived peptide-1 (SSp-1 RLNEVAKNL), induced peptide-specific CTLs both in vivo (transgenic mice) and in vitro (human PBLs), which specifically released interferon-gamma (IFN-gamma) upon stimulation with SSp-1-pulsed autologous dendritic cells (DCs) or T2 cells. SSp-1-specific CTLs also lysed major histocompatibility complex (MHC)-matched tumor cell lines engineered to express S proteins. HLA-A * 0201-SSp-1 tetramer staining revealed the presence of significant populations of SSp-1-specific CTLs in SSp-1-induced CD8+ T cells. We propose that the newly identified epitope SSp-1 will help in the characterization of virus control mechanisms and immunopathology in SARS-CoV infection, and may be relevant to the development of immunotherapeutic approaches for SARS.

  9. Comparative Proteomic Analysis of Lactobacillus casei XM2-1 between Exponential and Stationary Phases%干酪乳杆菌对数生长期与稳定期蛋白质组比较分析

    Institute of Scientific and Technical Information of China (English)

    乌日娜; 岳喜庆; 张和平

    2012-01-01

    研究建立干酪乳杆菌Lactobacillus casei XM2—1的蛋白质组双向电泳表达图谱,比较其在对数生长中期与稳定期初期的图谱差异。结果表明:有16个蛋白质点表达发生明显变化,经过基质辅助激光解吸,电离飞行时间质谱鉴定,其中有7个蛋白质点鉴定为应激蛋白质,分别为GroEL、DnaK、Hsp20—1、Hsp20—2和UspA蛋白质。可见,应激蛋白质可能在L-casei XM2-1稳定期对外界环境的抵御能力增强起到重要作用。%Proteomic analysis was carried out to identify and characterize protein expression in Lactobacillus casei XM2-1. The cytosolic proteins of L. casei XM2-1 at the mid-exponential phase and early stationary phase were resolved by two- dimensional gel electrophoresis. Sixteen proteins revealed significant differences between two growth phases, and seven proteins were identified as stress proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF/MS), which included GroEL, DnaK, Hsp 20-1, Hsp 20-2 and UspA. This will be helpful for improving our understanding of the lg-ATR response in L. casei.

  10. Effects of Lactobacillus casei on the fermentation properties and quality of goat yogurt%干酪乳杆菌对羊奶酸奶发酵特性及品质的影响

    Institute of Scientific and Technical Information of China (English)

    高微娟; 张富新; 魏怡; 吴月红; 陈伟

    2011-01-01

    Using goat milk as raw material, influence of Lactobacillus casei on fermentation properties and quality of goat yogurt was studied on the basis of adding traditional yogurt starter.The results showed that it was beneficial to the growth of Lactobacillus casei when the inoculation of Lactobacillus casei was more than 3%, whereas the growth of Streptococcus thermophilus and Lactobacillus bulgaricus was inhibited and the quality of goat yogurt was effected. When the fermentation temperature was higher than 39℃, the viable counts of Lactobacillus casei declined obviously ( P< 0. 05) , which was not favorable for forming good flavour of yogurt. High quality goat yogurt can be obtained when Lactobacillus casei inoculation was 3%~5 % and fermentation temperature was 39 ℃.%以羊奶为原料,在添加传统发酵剂的基础上研究了干酪乳杆菌对羊奶酸奶发酵特性及品质的影响.结果表明:干酪乳杆茵接种量大于3%时有利于羊奶酸奶中干酪乳杆茵的生长,但会抑制酸奶中嗜热链球茵和保加利亚乳杆茵的生长,进而影响羊奶酸奶的品质;发酵温度高于39℃时酸奶中干酪乳杆菌活茵数量显著减少(P<0.05),不利于酸奶良好风味的形成;当干酪乳杆菌的接种量为3%~5%、发酵温度为39℃时,羊奶酸奶的品质最好.

  11. Adhesion of Lactobacillus casei LC2W to different gastric cell lines%干酪乳杆菌LC2W对两种不同胃癌细胞的黏附作用

    Institute of Scientific and Technical Information of China (English)

    周方方; 郭本恒; 吴正钧; 陈臣; 陈雪华

    2007-01-01

    选取人胃癌细胞株MKN-45和HGC-27,采用形态学观察和显微计数的方法比较菌液浓度、悬浮液、pH值对干酪乳杆菌LC2W(Lactobacillus casei LC2W)黏附细胞的情况.结果表明,LC2W对两种细胞均具有黏附性,对MKN-45的黏附性更强;这种黏附作用具有菌悬液浓度依赖性.当加入的菌悬液浓度达到-定数量时(109mL-1),平均每个细胞黏附的乳杆菌数量趋于饱和;悬浮介质对Lb.casei LC2W的黏附性具有明显的影响,其中悬浮于耗尽培养上清液(SCS)的Lb.casei LC2W表现出最好的黏附性;悬浮介质的pH值对Lb casei LC2W的黏附性也有影响,pH=4.0时黏附性最好.将不同悬浮介质的pH值调整到pH=7.0后,SCS仍能很好的促进LC2W的黏附作用,表明SCS中存在某种促进黏附的物质.由此推测,Lb.casei LC2W对胃癌细胞的黏附机理可能为黏附素与受体的特异性结合.

  12. The anti-obesity effects of Lactobacillus casei strain Shirota versus Orlistat on high fat diet-induced obese rats

    Directory of Open Access Journals (Sweden)

    Golgis Karimi

    2015-12-01

    Full Text Available Background: Obesity and overweight are major public health problems. Various factors, such as daily nutritional habits, physical inactivity, and genetic, are related to the prevalence of obesity. Recently, it was revealed that the gut microflora may also play an important role in weight management. Thus, this study aimed to determine the anti-obesity effects of Lactobacillus casei strain Shirota (LcS compared with those of orlistat in an animal model fed a high-fat diet (HFD. Design: Thirty-two male Sprague-Dawley rats were assigned to four groups fed various diets as follows: a standard diet group, HFD group, HFD supplemented with LcS (108109 colony-forming units (HFD-LcS group, and HFD group treated with Orlistat (10 mg/kg body weight. After 15 weeks, the weights of organs, body weight, body fat mass and serological biomarkers were measured. In addition, histological analysis of the liver and adipose tissue was performed. Results: Body weight, body mass index, fat mass, leptin and glucose levels were lower, and high-density lipoprotein and adiponectin levels were higher in the HFD-LcS and HFD-orlistat groups than in the HFD group. In addition a significant difference in body fat mass was observed between HFD-LcS group with HFD-orlistat group (19.19±5.76 g vs. 30.19±7.98 g. Although the interleukin-6 level was significantly decreased in the HFD-LcS and HFD-orlistat groups compared with the HFD group, no significant change was observed in other inflammatory biomarkers. Conclusion: The results of the present study show that LcS supplementation improves body weight management and the levels of some related biomarkers. In addition, LcS supplementation showed a better result in fat mass and alanine aminotransferase reduction than Orlistat. Further studies are needed to elucidate the anti-obesity effects of LcS, with a longer period of supplementation.

  13. Probiotic “requeijão cremoso”: evaluation of Lactobacillus casei viability, physical-chemical composition and sensory acceptance Requeijão cremoso probiótico: avaliação da viabilidade de Lactobacillus casei, da composição físico-química e aceitação sensorial

    Directory of Open Access Journals (Sweden)

    Priscila Cristina Bizam Vianna

    2012-02-01

    Full Text Available The objective of this work was the production of probiotic “requeijão cremoso” that keeps the physicalchemical and sensorial characteristics of the tradicional product, linked to the functional properties. Control (without probiotic addition and probiotic “requeijão cremoso” added with Lactobacillus casei after fusion, at 50±1ºC, were produced, packed and stored at 4±1ºC. Control and probiotic “requeijão cremoso” were evaluated to physical-chemical composition 1 day after processing. After 1, 7, 14 and 28 days of storage, the pH were evaluated for both products and Lactobacillus casei count only for probiotic “requeijão cremoso”. Sensory evaluation was accomplished on the 4th day of storage for appearance, taste, flavor, texture, general acceptance and purchasing intention. The results were evaluated by analysis of variance and Tukey’s test. There was no difference on physical-chemical composition of control and probiotic “requeijão cremoso”. Lactobacillus casei count was 2,2x107 UFC/g after 1 day of processing and not showed significant variations during the 28 days of storage. The probiotic “requeijão cremoso” presented good sensory acceptance and 82% of the consumers certainly or probably buy the probiotic product. The results showed that “requeijão cremoso” may regard as a good alternative of probiotic product.O objetivo deste trabalho foi a elaboração de um requeijão cremoso probiótico, que mantenha as características físico-químicas e sensoriais do produto tradicional, agregando propriedades funcionais. Foi produzido um requeijão cremoso controle (sem a adição de probiótico e outro adicionado de Lactobacillus casei, após a fusão da massa, à temperatura de 50±1ºC. Os requeijões controle e probiótico foram envasados, armazenados a 4±1ºC e submetidos às análises de composição centesimal após 1 dia de processamento. O pH dos requeijões, assim como a contagem de Lactobacillus

  14. Ontogenetic differences in mesophyll structure and chlorophyll distribution in Eucalyptus globulus ssp. globulus.

    Science.gov (United States)

    James, S A; Smith, W K; Vogelmann, T C

    1999-02-01

    Mesophyll structure has been associated with the photosynthetic performance of leaves via the regulation of internal light and CO(2) profiles. Differences in mesophyll structure and chlorophyll distribution within three ontogenetically different leaf types of Eucalyptus globulus ssp. globulus were investigated. Juvenile leaves are blue-grey in color, dorsiventral (adaxial palisade layer only), hypostomatous, and approximately horizontal in orientation. In contrast, adult leaves are dark green in color, isobilateral (adaxial and abaxial palisade), amphistomatous, and nearly vertical in orientation. The transitional leaf type has structural features that appear intermediate between the juvenile and adult leaves. The ratio of mesophyll cell surface area per unit leaf surface area (A(mes)/A) of juvenile leaves was maximum at the base of a single, adaxial palisade layer and declined through the spongy mesophyll. Chlorophyll a + b content showed a coincident pattern, while the chlorophyll a:b ratio declined linearly from the adaxial to abaxial epidermis. In comparison, the mesophyll of adult leaves had a bimodal distribution of A(mes)/A, with maxima occurring beneath both the adaxial and abaxial surfaces within the first layer of multiple palisade layers. The distribution of chlorophyll a + b content had a similar pattern, although the maximum ratio of chlorophyll a:b occurred immediately beneath the adaxial and abaxial epidermis. The matching distributions of A(mes)/A and chlorophyll provide further evidence that mesophyll structure may act to influence photosynthetic performance. These changes in internal leaf structure at different life stages of E. globulus may be an adaptation for increased xeromorphy under increasing light exposure experienced from the seedling to adult tree, similar to the characteristics reported for different species according to sunlight exposure and water availability within their native habitats.

  15. Systemic and mucosal immune reactivity upon Mycobacterium avium ssp. paratuberculosis infection in mice.

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    Arzu Koc

    Full Text Available Mycobacterium avium ssp. paratuberculosis (MAP is the cause of Johne's disease, an inflammatory bowel disorder of ruminants. Due to the similar pathology, MAP was also suggested to cause Crohn's disease (CD. Despite of intensive research, this question is still not settled, possibly due to the lack of versatile mouse models. The aim of this study was to identify basic immunologic mechanisms in response to MAP infection. Immune compromised C57BL/6 Rag2-/- mice were infected with MAP intraperitoneally. Such chronically infected mice were then reconstituted with CD4+ and CD8+ T cells 28 days after infection. A systemic inflammatory response, detected as enlargement of the spleen and granuloma formation in the liver, was observed in mice infected and reconstituted with CD4+ T cells. Whereby inflammation in infected and CD4+CD45RB(hi T cell reconstituted animals was always higher than in the other groups. Reconstitution of infected animals with CD8+ T cells did not result in any inflammatory signs. Interestingly, various markers of inflammation were strongly up-regulated in the colon of infected mice reconstituted with CD4+CD45RB(lo/int T cells. We propose, the usual non-colitogenic CD4+CD45RB(lo/int T cells were converted into inflammatory T cells by the interaction with MAP. However, the power of such cells might be not sufficient for a fully established inflammatory response in the colon. Nevertheless, our model system appears to mirror aspects of an inflammatory bowel disease (IBD like CD and Johne's diseases. Thus, it will provide an experimental platform on which further knowledge on IBD and the involvement of MAP in the induction of CD could be acquired.

  16. [Effects of ruta SSP on the activity of the smooth gastrointestinal muscle isolated of rat].

    Science.gov (United States)

    Grigorjev, Carlota A; Brizuela, Nilda Y

    2010-01-01

    Ruta graveolens L. and Ruta chalepensis L. are plants used in folkloric medicine as antispasmodics, digestive and for intestinal gases. Animals used as experimental model were rats of the Wistar line, adult females, clinically healthy and with a weight average of 250 grams. We used strips of stomach and duodenum. Each one of the segments mounted on two stirrups in a bath of organ isolated with Ringer-lactate solution, at 37° C , pH: 7.3-7.4, and bubbled with 95% O2, 5% CO2. One of the stirrups was connected vertically to the bottom of the bath and the other to a transducer of tension connected to a Beckman polygraph. We applied 500 mgs of basal tension. After the stabilization, the ethanolic extract of Ruta ssp was added in increasing doses. At 50μl/ml the tone lower 23% in small intestine and lower 27% in stomach. However at 100 μl/ml the tone lower 32% and 35% respectively. In the other parameters the amplitude decrease 50% in the stomach at dose of 5 μl/ml while in the small intestine the amplitude lower 60%. With 10 μl/ml the amplitude change in both organs ( 96% in small intestine, and 75% in stomach). The frequency changes in both organs ( 32% in small intestine, and 50% in stomach) at 10 μl/ml Rue showed decreased effects on isolated small intestine and stomach were is dose dependent, maybe we were demonstrated the effects digestive of Ruta.

  17. Flowering time in wild beet ( Beta vulgaris ssp. maritima) along a latitudinal cline

    Science.gov (United States)

    Dijk, Henk Van; Boudry, Pierre; McCombre, Helen; Vernet, Philippe

    The wild beet ( Beta vulgaris ssp. maritima, a perennial species from the Mediterranean and the European Atlantic coasts) shows marked variation in flowering time in terms of both the year of first flowering and flowering date in a given year. Much of this variability is related to latitude. Beta vulgaris plants flower either in the same year as they germinate or in their second year. This is mainly due to differences in their requirement for vernalization, which is determined by a single gene B/b and by quantitative trait loci. The more southern the origin of the plants, the less vernalization is required. Also the B allele, which cancels vernalization requirement completely, has a high frequency in the Mediterranean region, but is completely absent in the northern part of the distribution of this species. We found that flowering date variation in relation to the latitude of origin is maintained under greenhouse conditions but does not follow a simple clinal relationship. From the Mediterranean northwards to the west coast of Brittany, flowering occurs progressively earlier, but from Brittany northwards to south-east England and The Netherlands it is progressively later. A possible explanation for this difference is that in the southern part of the range sensitivity to daylength and warmth control flowering time, whereas further north vernalization requirement is also a key factor. A substantial part of all differences in flowering time was heritable: heritability within populations was measured as 0.33 under greenhouse conditions. The high heritability implies evolutionary change may occur in this character.

  18. Khorasan wheat population researching (Triticum turgidum, ssp. Turanicum (McKey in the minimum tillage conditions

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    Ikanović Jela

    2014-01-01

    Full Text Available Khorasan wheat occupies a special place in the group of new-old cereals (Triticum turgidum, ssp. Turanicum McKey. It is an ancient species, native to eastern Persia, that is very close to durum wheat by morphological characteristics. Investigations were carried out in agro ecological conditions of the eastern Srem, with two wheat populations with dark and bright awns as objects of study. The following morphological and productive characteristics were investigated: plant height (PH, spike length (SH, number of spikelets per spike (NSS, absolute weight (AW and grain weight per spike (GW, seed germination (G and grains yield (YG. Field micro-experiments were set on the carbonate chernozem soil type on loess plateau in 2011 and 2012. Hand wheat sowing was conducted in early March with drill row spacing of 12 cm. The experiment was established as complete randomized block system with four replications. Tending crops measures were not applied during the growing season. Plants were grown without usage of NPK mineral nutrients. Chemical crop protection measures were not applied, although powdery mildew (Erysiphe graminis was appeared before plants spike formation in a small extent. The results showed that both populations have a genetic yield potential. In general, both populations manifested a satisfactory tolerance on lodging and there was no seed dispersal. Plants from bright awns population were higher, had longer spikes and larger number of spikelet’s per spike. However, plants from dark awns population had higher absolute weight and grains weight per spike, as well as grain yield per plant. Strong correlation connections were identified among the investigated characteristics. The determination of correlations, as well as direct and indirect affects, enabled easier understanding of the mutual relationships and their balancing in order to improve the yield per unit area. [Projekat Ministarstva nauke Republike Srbije, br. TR 31078 i br. TR 31022

  19. A draft sequence of the rice(Oryza sativa ssp. indica) genome

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have produced a draft rice genome sequence of Oryza sativa ssp. indica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM- BLER, a custom-designed software package used for the initial assembly, were verified rigorously by comparisons against finished BAC clone sequences from both indica and japanica strains, available from the public databases. Over all, 96.3% of full-length cDNAs, 96.4% of STS, STR, RFLP markers, 94.0% of ESTs and 94.9% unigene clusters were identified from the draft sequence. Our preliminary analysis on the data set shows that our rice draft sequence is consistent with the comman standard accepted by the genome sequencing community. The unconditional release of the draft to the public also undoubtedly provides a fundamental resource to the international scientific communities to facilitate genomic and genetic studies on rice biology.

  20. Salmonella enterica ssp. arizonae infection in a 43-year-old Italian man with hypoglobulinemia: a case report and review of the literature

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    Noto Pasquale

    2011-07-01

    Full Text Available Abstract Introduction Salmonella enterica ssp. arizonae is an uncommon human pathogen with serious infections reported in immunocompromised hosts. In Europe, only a few cases have been described. Patients with this infection usually have a history of contact with reptiles or travel abroad. We present a case report of infection in a patient with hypoglobulinemia and a literature review. Case presentation We describe the case of a 43-year-old Caucasian Italian man with hypoglobulinemia who presented to our hospital with sepsis and diarrhea. A stool culture yielded S. enterica ssp. arizonae. Our patient was treated with oral ciprofloxacin and made a full recovery. We also present a review of the cases of S. enterica ssp. arizonae infections previously reported in Europe. Conclusions The majority of infections from S. enterica ssp. arizonae occur in patients who are immunocompromised. Data from the literature suggests that it may be difficult to eradicate the bacteria and thus, prolonged antibiotic courses are often used. It would be advisable for clinicians to investigate for pre-existing immune dysfunction if S. enterica ssp. arizonae is isolated. In Italy, although there have only been a few cases, the likely route of transmission remains unclear and requires further surveillance.

  1. The development-specific protein (Ssp1) from Sclerotinia sclerotiorum is encoded by a novel gene expressed exclusively in sclerotium tissues.

    Science.gov (United States)

    Li, Moyi; Rollins, Jeffrey A

    2009-01-01

    The gene encoding a development-specific protein (Ssp1) was identified; it previously was described as the major protein present in mature sclerotia of Sclerotinia sclerotiorum. To determine the developmental specificity of ssp1 gene expression in relation to protein accumulation we examined transcript and protein accumulation during various growth and development stages of the lifecycle. We found that ssp1 transcript accumulated exclusively within developing sclerotium tissue and not in any other examined stage of growth or development. In contrast high levels of Sspl protein were detectable by western blot and tandem mass spectrometry analyses in all stages of sclerotium as well as apothecium development. Immunolocalization further indicated that Ssp1 protein bodies were depleted from the sclerotium tissue surrounding the site of apothecium germination, but by this method Sspl was not detected in the apothecium. Together these findings suggest that Sspl is not metabolized during carpogenic germination, instead it is translocated from the sclerotium to the apothecium in an antigenically novel form. Outside the Sclerotiniaceae ssp1 homologs were found only from the sclerotium-forming Aspergillus species A. flavus and A. oryzae. Further studies concerning the regulation and function of this gene and its occurrence in other species have the potential to inform our understanding of sclerotium development and the evolution of sclerotia and other forms of fungal stroma. PMID:19271669

  2. Potential Nociceptive Regulatory Effect of Probiotic Lactobacillus rhamnosus PB01 (DSM 14870) on Mechanical Sensitivity in Diet-Induced Obesity Model

    Science.gov (United States)

    Brandsborg, Erik

    2016-01-01

    Treatments for obesity have been shown to reduce pain secondary to weight loss. Intestinal microbiota, as an endogenous factor, influences obesity and pain sensitivity but the effect of oral probiotic supplementation on musculoskeletal pain perception has not been studied systematically. The present study examined the effect of a single daily oral dose (1 × 109 CFU) of probiotics (Lactobacillus rhamnosus PB01, DSM14870) supplement on mechanical pain thresholds in behaving diet-induced obese (DIO) mice and their normal weight (NW) controls. The mice (N = 24, 6-week-old male) were randomly divided into four groups on either standard or high fat diet with and without probiotic supplementation. Both DIO and NW groups with probiotic supplementation maintained an insignificant weight gain while the control groups gained significant weight (P < 0.05). Similarly, both DIO and NW probiotics supplemented groups demonstrated a significantly (P < 0.05) lower sensitivity to mechanical stimulation compared to their corresponding control. The results of this study suggest a protective effect of probiotics on nociception circuits, which propose a direct result of the weight reduction or an indirect result of anti-inflammatory properties of the probiotics. Deciphering the exact underlying mechanism of the weight loss and lowering nociception effect of the probiotic applied in this study require further investigation. PMID:27647980

  3. Potential Nociceptive Regulatory Effect of Probiotic Lactobacillus rhamnosus PB01 (DSM 14870 on Mechanical Sensitivity in Diet-Induced Obesity Model

    Directory of Open Access Journals (Sweden)

    Fereshteh Dardmeh

    2016-01-01

    Full Text Available Treatments for obesity have been shown to reduce pain secondary to weight loss. Intestinal microbiota, as an endogenous factor, influences obesity and pain sensitivity but the effect of oral probiotic supplementation on musculoskeletal pain perception has not been studied systematically. The present study examined the effect of a single daily oral dose (1 × 109 CFU of probiotics (Lactobacillus rhamnosus PB01, DSM14870 supplement on mechanical pain thresholds in behaving diet-induced obese (DIO mice and their normal weight (NW controls. The mice (N=24, 6-week-old male were randomly divided into four groups on either standard or high fat diet with and without probiotic supplementation. Both DIO and NW groups with probiotic supplementation maintained an insignificant weight gain while the control groups gained significant weight (P<0.05. Similarly, both DIO and NW probiotics supplemented groups demonstrated a significantly (P<0.05 lower sensitivity to mechanical stimulation compared to their corresponding control. The results of this study suggest a protective effect of probiotics on nociception circuits, which propose a direct result of the weight reduction or an indirect result of anti-inflammatory properties of the probiotics. Deciphering the exact underlying mechanism of the weight loss and lowering nociception effect of the probiotic applied in this study require further investigation.

  4. Microencapsulation of Lactobacillus rhamnosus GG by Transglutaminase Cross-Linked Soy Protein Isolate to Improve Survival in Simulated Gastrointestinal Conditions and Yoghurt.

    Science.gov (United States)

    Li, Chun; Wang, Chun-Ling; Sun, Yu; Li, Ai-Li; Liu, Fei; Meng, Xiang-Chen

    2016-07-01

    Microencapsulation is an effective way to improve the survival of probiotics in simulated gastrointestinal (GI) conditions and yoghurt. In this study, microencapsulation of Lactobacillus rhamnosus GG (LGG) was prepared by first cross-linking of soy protein isolate (SPI) using transglutaminase (TGase), followed by embedding the bacteria in cross-linked SPI, and then freeze-drying. The survival of microencapsulated LGG was evaluated in simulated GI conditions and yoghurt. The results showed that a high microencapsulation yield of 67.4% was obtained. The diameter of the microencapsulated LGG was in the range of 52.83 to 275.16 μm. Water activity did not differ between free and microencapsulated LGG after freeze-drying. The survival of microencapsulated LGG under simulated gastric juice (pH 2.5 and 3.6), intestinal juice (0.3% and 2% bile salt) and storage at 4 °C were significantly higher than that of free cells. The survival of LGG in TGase cross-linked SPI microcapsules was also improved to 14.5 ± 0.5% during storage in yoghurt. The microencapsulation of probiotics by TGase-treated SPI can be a suitable alternative to polysaccharide gelation technologies. PMID:27228279

  5. Potential Nociceptive Regulatory Effect of Probiotic Lactobacillus rhamnosus PB01 (DSM 14870) on Mechanical Sensitivity in Diet-Induced Obesity Model.

    Science.gov (United States)

    Dardmeh, Fereshteh; Nielsen, Hans Ingolf; Alipour, Hiva; Kjærgaard, Benedict; Brandsborg, Erik; Gazerani, Parisa

    2016-01-01

    Treatments for obesity have been shown to reduce pain secondary to weight loss. Intestinal microbiota, as an endogenous factor, influences obesity and pain sensitivity but the effect of oral probiotic supplementation on musculoskeletal pain perception has not been studied systematically. The present study examined the effect of a single daily oral dose (1 × 10(9) CFU) of probiotics (Lactobacillus rhamnosus PB01, DSM14870) supplement on mechanical pain thresholds in behaving diet-induced obese (DIO) mice and their normal weight (NW) controls. The mice (N = 24, 6-week-old male) were randomly divided into four groups on either standard or high fat diet with and without probiotic supplementation. Both DIO and NW groups with probiotic supplementation maintained an insignificant weight gain while the control groups gained significant weight (P < 0.05). Similarly, both DIO and NW probiotics supplemented groups demonstrated a significantly (P < 0.05) lower sensitivity to mechanical stimulation compared to their corresponding control. The results of this study suggest a protective effect of probiotics on nociception circuits, which propose a direct result of the weight reduction or an indirect result of anti-inflammatory properties of the probiotics. Deciphering the exact underlying mechanism of the weight loss and lowering nociception effect of the probiotic applied in this study require further investigation. PMID:27647980

  6. Evaluation of the wood CCA preservative treatment process of Eucalyptus (Eucaliptus ssp) by X-ray fluorescence technique

    International Nuclear Information System (INIS)

    Brazil produces around 1,2 mi m3 of treated wood to meet the annual demand of railway, electric, rural and construction sectors. The treated woods used for poles, sleepers, fence posts and plywoods should be according to Brazilian norms requirements. The most used wood species are eucalyptus (Eucaliptus ssp)and pine (Pinus ssp). The most wood preservative products used in Brazil are CCA (Chromated Copper Arsenate) and CCB (Copper Chromium and Boron Salt). The analytical methods, such as Flame Atomic Absorption Spectrometry (FAAS) and Plasma Inductively Coupled Optical Emission Spectrometry (ICPOES) have been used for the evaluation of those treatment processes. In this work, the sapwood sample was obtained from eucalyptus trees (Eucaliptus ssp) obtained from Minas Gerais State, Brazil, cut plantation areas. Sawdust sapwood sample was grounded and submitted to different additions of CCA solutions (0.2, 0.7, 1.3, 2.3, 3.6, 6.3, 11.7and17.9 kg m-3). Power and pressed pellets sapwood samples, analyzed by EDXRFS, showed a good linear relation (r2>0.99) between the characteristic intensity fluorescent lines (CuΚα, CrΚαand AsΚΒ) and their concentration, also, showed adequate sensitivity (LQ -1) for Cu, Cr and As determination in treated woods. Cu, Cr and As were determined in powdered sawdust samples by FAA spectrometry, using the AWPA A11-93 standard method; the relation between the CCA retention and their concentration showed a lower linear relation than EDXRFS; the FAAS spreading result could be attributed to laboratorial CCA addition process. (author)

  7. Evaluation of the wood CCA preservative treatment process of Eucalyptus (Eucaliptus ssp) by X-ray fluorescence technique

    Energy Technology Data Exchange (ETDEWEB)

    Pereira Junior, Sergio Matias, E-mail: matias@ipt.br [Instituto de Pesquisas Tecnologicas (IPT), Sao Paulo, SP (Brazil); Salvador, Vera Lucia Ribeiro; Sato, Ivone Mulako, E-mail: imsato@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    Brazil produces around 1,2 mi m{sup 3} of treated wood to meet the annual demand of railway, electric, rural and construction sectors. The treated woods used for poles, sleepers, fence posts and plywoods should be according to Brazilian norms requirements. The most used wood species are eucalyptus (Eucaliptus ssp)and pine (Pinus ssp). The most wood preservative products used in Brazil are CCA (Chromated Copper Arsenate) and CCB (Copper Chromium and Boron Salt). The analytical methods, such as Flame Atomic Absorption Spectrometry (FAAS) and Plasma Inductively Coupled Optical Emission Spectrometry (ICPOES) have been used for the evaluation of those treatment processes. In this work, the sapwood sample was obtained from eucalyptus trees (Eucaliptus ssp) obtained from Minas Gerais State, Brazil, cut plantation areas. Sawdust sapwood sample was grounded and submitted to different additions of CCA solutions (0.2, 0.7, 1.3, 2.3, 3.6, 6.3, 11.7and17.9 kg m{sup -3}). Power and pressed pellets sapwood samples, analyzed by EDXRFS, showed a good linear relation (r{sup 2}>0.99) between the characteristic intensity fluorescent lines (CuΚα, CrΚαand AsΚΒ) and their concentration, also, showed adequate sensitivity (LQ < 5mgkg{sup -1}) for Cu, Cr and As determination in treated woods. Cu, Cr and As were determined in powdered sawdust samples by FAA spectrometry, using the AWPA A11-93 standard method; the relation between the CCA retention and their concentration showed a lower linear relation than EDXRFS; the FAAS spreading result could be attributed to laboratorial CCA addition process. (author)

  8. Aspectos ecológicos y distribución de Tropaeolum tuberosum ssp. silvestre (Tropaeolaceae) en Argentina

    OpenAIRE

    Eva Bulacio; Hugo Ayarde

    2012-01-01

    Tropaeolum tuberosum Ruiz et Pav. ssp. silvestre Sparre es una hierba rizomatosa trepadora que en Argentina fue encontrada en las Sierras de Calilegua (Jujuy) y en áreas montanas de Catamarca, aunque de esta última localidad sólo se conoce un único e incompleto ejemplar. Habita quebradas húmedas, entre los 2.100 y 2.650 m s.n.m., en el límite superior del bosque montano. El tallo es grácil de hasta 3 m de largo y la porción subterránea está constituida por rizomas alargados y de hasta 1 cm de...

  9. Analysis of expressed sequence tags from Musa acuminata ssp. burmannicoides, var. Calcutta 4 (AA) leaves submitted to temperature stresses

    OpenAIRE

    Santos, C R; Martins, N.F.; Horberg, H.M.; Almeida, E.R.P.; Coelho, M.C.F.; Togawa, R.; F. R. Silva; Caetano, A.R.; Miller, R.N.G.; de Souza, M T

    2005-01-01

    In order to discover genes expressed in leaves of Musa acuminata ssp. burmannicoides var. Calcutta 4 (AA), from plants submitted to temperature stress, we produced and characterized two full-length enriched cDNA libraries. Total RNA from plants subjected to temperatures ranging from 5°C to 25°C and from 25°C to 45°C was used to produce a COLD and a HOT cDNA library, respectively. We sequenced 1,440 clones from each library. Following quality analysis and vector trimming, we assembled 2,286 se...

  10. L-乳酸细菌培养条件的优化%Optimizing Culture Condition of L - lactic Acid Bacteria Lactobacillus casei FH- 2

    Institute of Scientific and Technical Information of China (English)

    李宝库; 马珦玻; 路新利; 魏志文

    2005-01-01

    实验通过对L-乳酸细菌(Lactobacillus casei FH-2)菌体生长、产酸速率、耐酸水平、高糖发酵性能的优化,实现了优化该菌生长和条件的目的.并对培养基中不同辅料的添加进行了研究.结果证明:共同加入麦根-麸皮浸泡液时,可以提高乳酸的产量,其最佳方案为50℃、4h、1%麸皮浸泡液和2%麦根.乳酸产量为40.5g/L.结论:实验通过优化L-乳酸细菌的发酵条件提高了乳酸的产量.

  11. SspA对生物材料细菌黏附及生物膜形成的影响%Influence of SspA on bacterial adherence and formation of bacterial biofilm to biomaterials

    Institute of Scientific and Technical Information of China (English)

    林兴; 黄云超; 杨达宽; 奚廷斐

    2005-01-01

    金黄色葡萄球菌(staphylococcus aureus,SA)是以生物材料为中心感染的主要致病菌之一,主要通过细菌黏附并形成生物膜造成感染,给患者带来灾难性的后果.SA的表面蛋白纤维连接蛋白结合蛋白(fibronectin-binding protein,FnBP)是其关键的黏附因子,而金黄色葡萄球菌丝氨酸蛋白酶(staphylococcal serine protease,SspA)可降低细菌的FnBP,抑制自溶素(autolysin,AtlE)分泌,降解表面A蛋白(surfaceproteinA,Spa)从而抑制细菌黏附和生物膜(bacterial biofilm,BF)的形成,为SspA在控制SA感染方面提供了一个重要的治疗途径.

  12. Effector MiSSP7 of the mutualistic fungus Laccaria bicolor stabilizes the Populus JAZ6 protein and represses jasmonic acid (JA) responsive genes.

    Science.gov (United States)

    Plett, Jonathan M; Daguerre, Yohann; Wittulsky, Sebastian; Vayssières, Alice; Deveau, Aurelie; Melton, Sarah J; Kohler, Annegret; Morrell-Falvey, Jennifer L; Brun, Annick; Veneault-Fourrey, Claire; Martin, Francis

    2014-06-01

    Ectomycorrhizal fungi, such as Laccaria bicolor, support forest growth and sustainability by providing growth-limiting nutrients to their plant host through a mutualistic symbiotic relationship with host roots. We have previously shown that the effector protein MiSSP7 (Mycorrhiza-induced Small Secreted Protein 7) encoded by L. bicolor is necessary for the establishment of symbiosis with host trees, although the mechanistic reasoning behind this role was unknown. We demonstrate here that MiSSP7 interacts with the host protein PtJAZ6, a negative regulator of jasmonic acid (JA)-induced gene regulation in Populus. As with other characterized JASMONATE ZIM-DOMAIN (JAZ) proteins, PtJAZ6 interacts with PtCOI1 in the presence of the JA mimic coronatine, and PtJAZ6 is degraded in plant tissues after JA treatment. The association between MiSSP7 and PtJAZ6 is able to protect PtJAZ6 from this JA-induced degradation. Furthermore, MiSSP7 is able to block--or mitigate--the impact of JA on L. bicolor colonization of host roots. We show that the loss of MiSSP7 production by L. bicolor can be complemented by transgenically varying the transcription of PtJAZ6 or through inhibition of JA-induced gene regulation. We conclude that L. bicolor, in contrast to arbuscular mycorrhizal fungi and biotrophic pathogens, promotes mutualism by blocking JA action through the interaction of MiSSP7 with PtJAZ6. PMID:24847068

  13. Milk digesta and milk protein fractions influence the adherence of Lactobacillus gasseri R and Lactobacillus casei FMP to human cultured cells.

    Science.gov (United States)

    Volstatova, Tereza; Havlik, Jaroslav; Potuckova, Miroslava; Geigerova, Martina

    2016-08-10

    Adhesion to the intestinal epithelium is considered an important feature of probiotic bacteria, which may increase their persistence in the intestine, allowing them to exert their beneficial health effect or promote the colonisation process. However, this feature might be largely dependent on the host specificity or diet. In the present study, we investigated the effect of selected milks and milk protein fractions on the ability of selected lactobacilli to adhere to the cells of an intestinal model based on co-culture Caco-2/HT29-MTX cell lines. Most milk digesta did not significantly affect bacterial adhesion except for UHT-treated milk and sheep milk. The presence of UHT-treated milk digesta reduced the adhesion of Lactobacillus gasseri R by 61% but not that of Lactobacillus casei FMP. However, sheep milk significantly increased the adherence of L. casei FMP (P protein fractions, rennet casein (RCN) and bovine serum albumin (BSA) showed reproducible patterns and strain-specific effects on bacterial adherence. While RCN reduced the adherence of L. gasseri R to Whey protein (WH) tended to increase the adherence of both strains by 130%-180%. Recently, interactions between the host diet and its microbiota have attracted considerable interest. Our results may explain one of the aspects of the role of milk in the development of microbiota or support of probiotic supplements. Based on our data, we conclude that the persistence of probiotic strains supplemented as part of dairy food or constitutional microbiota in the gut might be affected negatively or positively by the food matrix through complex strain or concentration dependent effects. PMID:27435508

  14. Effect of Inoculation Amount of Lactobacillus casei on Quality Characteristics of Fermented Beef Kebabs%干酪乳杆菌添加量对发酵牛肉串品质特性的影响

    Institute of Scientific and Technical Information of China (English)

    张金铎; 孔保华; 夏秀芳; 施雪

    2012-01-01

    研究干酪乳杆菌的添加量对发酵牛肉串理化性质和感官特性的影响。将不同添加量的干酪乳杆菌分别与木糖葡萄球菌复配添加到牛肉中生产发酵牛肉串,测定发酵牛肉串在成熟过程中的pH值、水分活度(aw)、颜色、质构及感官指标的变化。添加干酪乳杆菌显著改善发酵牛肉串的品质,并且延长了货架期。菌种添加量107CFU/g和108CFU/g时牛肉串在发酵过程中气味、滋味、颜色、嫩度、黏聚性和咀嚼性差异不显著,而菌种添加量108CFU/g时发酵牛肉串总体可接受性较差,酸味较重。因此,确定发酵牛肉串中干酪乳杆菌的添加量为107CFU/g,发酵终点时牛肉串获得了较好的质量。%The present study aimed to examine the effect of different inoculation amounts of Lactobacillus casei on physicochemical properties and sensory characteristics of fermented beef kebabs.Beef was fermented with different amounts of Lactobacillus casei and 107 CFU/g of S.xylosus to produce fermented beef kebabs.We analyzed changes in pH,water activity,color,texture and sensory characteristics during the fermentation process.Compared with S.xylosus alone,cofermentation with Lactobacillus casei and S.xylosus could significantly the quality of fermented beef kebabs and prolong its shelf life.Beef kebabs fermented with 107CFU/g and 108CFU/g of Lactobacillus casei showed no significant differences in smell,taste,color,tenderness,cohesiveness and chewiness.Beef kebabs fermented with 108CFU/g of Lactobacillus casei had poor overall acceptance and tasted sour.By contrast,better quality of fermented beef kebabs was achieved when the inoculum amount of Lactobacillus casei was 107CFU/g.

  15. A new synonym of Hylodesmum podocarpum ssp.podocarpum (Leguminosae)%圆菱叶长柄山蚂蝗一新异名

    Institute of Scientific and Technical Information of China (English)

    高信芬

    2004-01-01

    Close examination of the type material of Podocarpium lancangense Y. Y. Qian has shown that this species is indistinguishable from Hylodesmum podocarpum (DC.) H. Ohashi & R. R. Mill ssp. Podocarpum, and thus is reduced to synonymy herein.%查阅了澜沧长柄山蚂蝗Hylodesmum lancangense (Y. Y. Qian) X. Y. Zhu & H. Ohashi的模式标本后,确认该种应归并入圆菱叶长柄山蚂蝗H. podocarpum (DC.) H. Ohashi & R. R. Mill ssp. podocarpum.

  16. Subsurface irrigation of potato crop (Solanum tuberosum ssp. Andigena) in Suka Kollus with different drainage systems

    Science.gov (United States)

    Serrano-Coronel, Genaro; Chipana-Rivera, René; Fátima Moreno-Pérez, María; Roldán-Cañas, José

    2016-04-01

    Among the most important hydraulic structures of pre-Hispanic ancestral technology developed in the Andean region, we find the suka kollus, aymara word, called also waru waru, en quechua or raised fields, in English. They are raised platforms surrounded by water canals that irrigate subsurface, but also have the function of draining, to deal with floods because they are surrounding Lake Titicaca. They also have the property of generating a thermoregulatory effect to crops, depending on the configuration of the channels and platforms. Such agro-ecosystems are being abandoned, however, if properly addressed crop management and some drainage canals are replaced by underground drains for increased crop area could be very useful in enabling marginal soils affected by salts and / or excess water. For these reasons, the objective of this study was to evaluate the subsurface irrigation in the potato crop in suka kollus under a system of surface drainage, and mixed drainage (surface and subsurface). The study was conducted in marginal soils of Kallutaca area, located 30 km from the city of La Paz, Bolivia, at a height of 3892 m.a.s.l. The cultivation of the potato (Solanum tuberosum ssp. Andigena) was used. Four treatments were tested with different widths of the platforms: T1 (Control) with drainage through channels; T2 (replacing a channel by a drain); T3 (replacing two channels by two drains); T4 (replacing three channels by three drains). The flow of water into the soil from the water table was predominantly upward, except during periods of high rainfall. In terms of treatments, the flow in T1 was higher, mainly at weeks 8 to 11 after seedling emergence, coinciding with the phenological phases of flowering and at the beginning of the tuber ripening. It was followed by T3, T2 and T4 treatments, respectively. Tuber yield, if one considers that the channels detract arable land, was higher in the T3 treatment,16.4 Mg / ha, followed by T2 treatment, 15.2 Mg / ha, T1

  17. Fertilization in Brassica campestris ssp.pekinensis and its duration of each stage

    Institute of Scientific and Technical Information of China (English)

    PENG Jie; SHEN Jiaheng

    2007-01-01

    This paper reports the process of fertilization in Brassica campestris ssp.pekinensis and the duration of each stage.The results are as follows:(1)Pollen germinates on stigma 2-3 h after pollination.(2)4-8 h after pollination,pollen tube grows in the style.(3)8-14 h after pollination,pollen tube grows in the ovary and gets into the ovule via the micropyle.(4)16 h after pollination,one sperm nucleus moves to the egg and enters it.(5)The sperm nucleus adheres to the nuclear membrane of the egg 18 h after pollination.(6)20 h after pollination,it enters the egg nucleus and male chromatin gradually disperses and 24 h after pollination,a male nucleolus appears.A large female nucleolus and a small male nucleolus occur in the nucleus of the fertilized egg,and zygote formed.The dispersing of sperm chromatin in the egg nucleus takes about 4 h.(7)32--34 h after pollination,the division of zygote begins.The dormancy stage of the zygote lasts for about 8-10 h.(8)The pair polar nuclei lie in the chalazal end of the egg betbre fertilization,which may fuse into a secondary nucleus or not.(9)16-18 h after pollination,the sperm nucleus moves to the polar nuclei or the secondary nucleus.18 h after pollination,the sperm nucleus adheres to the nuclear membrane of the polar nuclei or that of the secondary nucleus.(10)20 h after pollination,the sperm nucleus enters one of the polar nuclei or the secondary nucleus and a triple fusion takes place.The process of fusion is similar to the karyogamy but faster.The dispersing of the sperm chromatin in the polar nucleus or secondary nucleus takes about 2 h.(11)22 h after pollination,the primary endosperm nucleus formed.The female and male nucleoli cannot fuse with each other betbre mitotic division of the primary endosperm nucleus.(12)24 h after pollination,the division of the primary endosperm nucleus actually takes place.

  18. A SNP and SSR based genetic map of asparagus bean (Vigna. unguiculata ssp. sesquipedialis and comparison with the broader species.

    Directory of Open Access Journals (Sweden)

    Pei Xu

    Full Text Available Asparagus bean (Vigna. unguiculata ssp. sesquipedialis is a distinctive subspecies of cowpea [Vigna. unguiculata (L. Walp.] that apparently originated in East Asia and is characterized by extremely long and thin pods and an aggressive climbing growth habit. The crop is widely cultivated throughout Asia for the production of immature pods known as 'long beans' or 'asparagus beans'. While the genome of cowpea ssp. unguiculata has been characterized recently by high-density genetic mapping and partial sequencing, little is known about the genome of asparagus bean. We report here the first genetic map of asparagus bean based on SNP and SSR markers. The current map consists of 375 loci mapped onto 11 linkage groups (LGs, with 191 loci detected by SNP markers and 184 loci by SSR markers. The overall map length is 745 cM, with an average marker distance of 1.98 cM. There are four high marker-density blocks distributed on three LGs and three regions of segregation distortion (SDRs identified on two other LGs, two of which co-locate in chromosomal regions syntenic to SDRs in soybean. Synteny between asparagus bean and the model legume Lotus. japonica was also established. This work provides the basis for mapping and functional analysis of genes/QTLs of particular interest in asparagus bean, as well as for comparative genomics study of cowpea at the subspecies level.

  19. Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients

    Directory of Open Access Journals (Sweden)

    Devraj J. Parasannanavar

    2013-01-01

    Full Text Available Background. Microlymphocytotoxicity (MLCT and flowcytometry (FC are the conventional serological methods to detect HLA-B* 27. Due to some disadvantages in these methods, most of the HLA laboratories have now switched over to molecular methods. Molecular techniques based on commercial kits are expensive; as such many laboratories with limited funds in developing countries cannot afford these techniques. Aims. Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for HLA-B* 27 typing. Materials and Methods. Sequence Specific primers were designed to amplify all the subtypes of B* 27 using IMGT-HLA sequence database. Accuracy was checked by retyping of 90 PCR-SSOP typed controls. Results. The presence of 149 bp specific band with control band on 2% agarose gel showed B* 27 positivity. No discrepancies were found when compared with PCR-SSOP results. The frequency of HLA-B* 27 was found to be significantly increased (68.75% versus 4.40%, O.R 46.909: P value 6.62E-32 among 700 SpA patients as compared to controls. Clinically, 54% of patients had polyarticular arthritis with SI joints involvement (68% and restricted spine flexion (60%. Conclusion. In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India.

  20. USE OF METHYL JASMONATE TO ENHANCE THE PRODUCTION OF 6-METHOXYPODOPHYLLOTOXIN IN CELL CULTURES OF LINUM THRACICUM SSP. THRACICUM

    Directory of Open Access Journals (Sweden)

    Pavlina Sasheva

    2015-09-01

    Full Text Available Secondary metabolites, such as lignans have important ecological role for plants and at the same time are lead structures for drug design in human medicine. The aryltetralin type of lignans are strong cytotoxic agents and are found in members of the genus Linum (Linaceae.Objective: In vitro cultures of Linum thracicum ssp. thracicum were developed to identify a medium type and an elicitation technique that favor enhanced production of the aryltetralin lignan 6-methoxypodophyllotoxin.Method: Methyl jasmonate and salicylic acid were administered to four cell lines of Linum thracicum ssp. thracicum for 24 and 72 hours. The 6-methoxypodophyllotoxin was identified by HPLS-ESI-MS/MS in positive ion mode, and its production was determined via quantitative HPLC analysis.Results: A cell line, called Li-20, which was developed in reduced sucrose environment proved to be the fastest growing and the highest producing among the tested cell lines. Within 24 hours upon MJ elicitation, the eight-day-old Li-20 increased 2.3-fold in 6-methoxypodophyllotoxin content, reaching 4.3 mg on a dry weight basis. Negative or no effect was registered after salicylic acid application.Conclusion: MJ elicitation is an effective strategy to improve the 6-methoxypodophyllotoxin accumulation within short periods of time, and an optimization of the cultivation medium beforehand is a prerequisite in the pipeline.

  1. Scientific Opinion on the substantiation of a health claim related to SYNBIO®, a combination of Lactobacillus rhamnosus IMC 501® and Lactobacillus paracasei IMC 502®, and maintenance of normal defecation pursuant to Article 13(5) of Regulation (EC) No

    DEFF Research Database (Denmark)

    Tetens, Inge

    on the scientific substantiation of a health claim related to SYNBIO®, a combination of Lactobacillus rhamnosus IMC 501® and Lactobacillus paracasei IMC 502®, and maintenance of normal defecation. The Panel considers that the food, SYNBIO®, which is the subject of the health claim, is sufficiently characterised...... for an effect of SYNBIO® on the maintenance of normal defecation in humans, studies which investigated the presence of L. rhamnosus IMC 501® and L. paracasei IMC 502® in the faeces of participants who consumed foods enriched with these strains were not considered by the Panel. The Panel concludes that a cause...

  2. 干酪乳杆菌LC2W发酵脱脂乳营养因子优化%Optimization of Nutritional Factors for Skim Milk Fermentation by Lactobacillus casei LC2W

    Institute of Scientific and Technical Information of China (English)

    杜昭平; 马成杰; 孙克杰; 龚广予; 刘鑫; 马爱民

    2012-01-01

    The effects of different types of added nitrogen source, carbon source and metal ion on the growth of L. casei LC2W in skim milk were evaluated to identify glucose, soybean polypeptide and MnSO4 as the best nutritional factors for L. casei LC2W. In addition, the logarithmic count of L. casei LC2W was investigated with respect to the concentrations of the selected nutritional factors. Response surface analysis based on a three-variable, three-level Box- Behnken design was employed to optimize these nutritional factors. MnSO4 concentration had the most important influence on the logarithmic count of L. casei LC2W followed by soybean polypeptide concentration; the effect of glucose concentration was the weakest. Moreover, a significant interaction between MnSO4 concentration and soybean polypeptide concentration was observed. The optimal concentrations of glucose, soybean polypeptide and MnSO4 were determined to be 65.90, 7.68 mg/L and 13.88 mg/L, respectively, yielding a L. casei LC2W count of 2.6×10^9 CFO/mL in 24 h of fermentation, which was 11.38 fold higher than before the optimization (2.1×10^8 CFU/mL).%通过向还原脱脂乳中添加碳源、氮源及金属离子,对干酪乳杆菌(L.casei)LC2W发酵脱脂乳营养因子进行优化。在单因素试验基础上,以脱脂乳中葡萄糖、大豆多肽和MnSO4添加量为自变量,以发酵乳中L.caseiLC2W菌落总数对数值为响应值,通过三因素三水平的Box-Behnken响应面分析法,分析各自变量及交互作用对L.caseiLC2W菌落总数的影响,并对营养因子进行优化。结果表明:各营养因子对L.caseiLC2W菌落总数的影响顺序为:MnSO4添加量〉大豆多肽添加量〉葡萄糖添加量,且MnSO4添加量与大豆多肽添加量的交互作用影响显著。各营养因子的优化参数为:葡萄糖添加量65.90g/L、大豆多肽添加量7.68g/L、MnSO4添加量13.88mg/L。在此优化条件下发酵24h后,干酪乳杆菌LC2

  3. Relative cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic Lactobacillus rhamnosus GG in managing infants with cow's milk allergy in Poland

    Directory of Open Access Journals (Sweden)

    Guest JF

    2016-06-01

    Full Text Available Julian F Guest,1,2 Diana Weidlich,1 Maciej Kaczmarski,3 Elzbieta Jarocka-Cyrta,4 Natalia Kobelska-Dubiel,5 Agnieszka Krauze,6 Iwona Sakowska-Maliszewska,7 Anna Zawadzka-Krajewska8 1Catalyst Health Economics Consultants, Northwood, Middlesex, 2Faculty of Life Sciences and Medicine, King’s College, London, UK; 3Department of Pediatrics, Gastroenterology, and Allergology, Medical University of Bialystok, Białystok, 4Uniwersytet Warmińsko‑Mazurski, Wydział Nauk Medycznych, Katedra Pediatrii Klinicznej, Olsztyn, 5Department of Pediatric Gastroenterology and Metabolic Diseases, Poznań University of Medical Sciences, Poznań, 6Klinika Pneumonologii i Alergologii Wieku Dziecięcego, Warsaw, 7Poradnia Gastroenterologiczna Centrum Pediatrii, Sosnowiec, 8Department of Pediatric Pneumonology and Allergology, Medical University of Warsaw, Warsaw, Poland Objective: To estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (eHCF containing the probiotic Lactobacillus rhamnosus GG (eHCF + LGG; Nutramigen LGG as an initial treatment for cow’s milk allergy compared with eHCF alone and amino acid formulas (AAF in Poland from the perspective of the Polish National Health Fund (Narodowy Fundusz Zdrowia [NFZ] and parents. Methods: Decision modeling was used to estimate the probability of cow’s milk allergic infants developing tolerance to cow’s milk by 18 months. The model also estimated the cost to the NFZ and parents (Polish Zloty [PLN] at 2013–2014 prices for managing infants over 18 months after starting one of the formulas as well as the relative cost-effectiveness of each of the formulas. Results: The probability of developing tolerance to cow’s milk by 18 months was higher among infants who were fed eHCF + LGG (0.82 compared with those fed eHCF alone (0.53 or an AAF (0.22. An infant who is initially managed with eHCF + LGG is expected to consume fewer health care resources than infants managed with the other

  4. Relative cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic Lactobacillus rhamnosus GG in managing infants with cow’s milk allergy in Spain

    Directory of Open Access Journals (Sweden)

    Guest JF

    2015-11-01

    Full Text Available Julian F Guest,1,2 Diana Weidlich,1 J Ignacio Mascuñan Díaz,3 Juan J Díaz,4 Pedro Manuel Ojeda,5 J Pablo Ferrer-González,6 David Gil,7 Isabel Onrubia Vaquero,8 Pedro Rincón Victor9 1Catalyst Health Economics Consultants, Northwood, Middlesex, 2Faculty of Life Sciences and Medicine, King’s College, London, UK; 3Centro de Salud de Paterna, Valencia, Spain; 4Hospital Universitario Central de Asturias, Oviedo, 5Clínica de Asma y Alergia Doctores Ojeda, 6Hospital General de Requena, Valencia, 7Hospital Universitario Virgen de la Arrixaca, Murcia, 8Centro de Salud Valle de la Oliva, Majadahonda, 9Centro de Salud la Chopera, Alcobendas, Spain Objective: To estimate the cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic Lactobacillus rhamnosus GG (eHCF + LGG; Nutramigen LGG as a first-line management for cow’s milk allergy compared with eHCF alone, and amino acid formulae in Spain, from the perspective of the Spanish National Health Service (SNS.Methods: Decision modeling was used to estimate the probability of immunoglobulin E (IgE-mediated and non–IgE-mediated allergic infants developing tolerance to cow’s milk by 18 months. The models also estimated the SNS cost (at 2012/2013 prices of managing infants over 18 months after starting a formula as well as the relative cost-effectiveness of each of the formulae.Results: The probability of developing tolerance to cow’s milk by 18 months was higher among infants with either IgE-mediated or non–IgE-mediated allergy who were fed eHCF + LGG compared with those fed one of the other formulae. The total health care cost of initially feeding infants with eHCF + LGG was less than that of feeding infants with one of the other formulae. Hence, eHCF + LGG affords the greatest value for money to the SNS for managing both IgE-mediated and non–IgE-mediated cow’s milk allergy.Conclusion: Using eHCF + LGG instead of eHCF alone or amino acid formulae for

  5. Crystallization and X-ray Crystallographic Analysis of the Adhesive SpaC Pilin Subunit in the SpaCBA Pilus of Gut-adapted Lactobacillus rhamnosus GG.

    Science.gov (United States)

    Kant, Abhiruchi; von Ossowski, Ingemar; Palva, Airi; Krishnan, Vengadesan

    2016-01-01

    Gram-positive Lactobacillus rhamnosus GG, a gut-adapted commensalic (and probiotic) strain, is known to express sortase-assembled pili on its cell surface. These SpaCBA-called pili consist of three different types of building blocks; the SpaA backbone-pilin subunit and the SpaB and SpaC ancillary pilins. SpaC is a relatively large (~90kDa) multi-domain fimbrial adhesin, and while it is located primarily at the SpaCBA pilus tip, occasionally, it can also be detected throughout the length of pilus backbone. Functionally, SpaC mainly accounts for SpaCBA pilus-mediated interactions with intestinal mucus, collagen, and human gut epithelial cells. Moreover, SpaC adhesiveness is also perceived to have a causal relationship with SpaCBA pilus-induced host-cell immune responses. In order to improve the mechanistic understanding of SpaC and its adhesive properties by structural investigation, we purified and successfully crystallized a recombinant construct of the near full-length SpaC protein (residues 36-856) in the presence of magnesium ions. X-ray diffraction data were collected to 2.6 Å resolution. The SpaC crystal belongs to the space group P21212 with unit cell parameters a = 116.5, b = 128.3, c = 136.5 Å and contains two molecules in the asymmetric unit. Presence of conserved metal ion-dependent adhesion site containing von Willebrand factor type A domain suggests its likely role in the function of SpaC. PMID:26732247

  6. A Lactobacillus rhamnosus GG-derived soluble protein, p40, stimulates ligand release from intestinal epithelial cells to transactivate epidermal growth factor receptor.

    Science.gov (United States)

    Yan, Fang; Liu, Liping; Dempsey, Peter J; Tsai, Yu-Hwai; Raines, Elaine W; Wilson, Carole L; Cao, Hailong; Cao, Zheng; Liu, LinShu; Polk, D Brent

    2013-10-18

    p40, a Lactobacillus rhamnosus GG (LGG)-derived soluble protein, ameliorates intestinal injury and colitis, reduces apoptosis, and preserves barrier function by transactivation of the EGF receptor (EGFR) in intestinal epithelial cells. The aim of this study is to determine the mechanisms by which p40 transactivates the EGFR in intestinal epithelial cells. Here we show that p40-conditioned medium activates EGFR in young adult mouse colon epithelial cells and human colonic epithelial cell line, T84 cells. p40 up-regulates a disintegrin and metalloproteinase domain-containing protein 17 (ADAM17) catalytic activity, and broad spectrum metalloproteinase inhibitors block EGFR transactivation by p40 in these two cell lines. In ADAM17-deficient mouse colonic epithelial (ADAM17(-/-) MCE) cells, p40 transactivation of EGFR is blocked, but can be rescued by re-expression with WT ADAM17. Furthermore, p40 stimulates release of heparin binding (HB)-EGF, but not transforming growth factor (TGF)α or amphiregulin, in young adult mouse colon cells and ADAM17(-/-) MCE cells overexpressing WT ADAM17. Knockdown of HB-EGF expression by siRNA suppresses p40 effects on transactivating EGFR and Akt, preventing apoptosis, and preserving tight junction function. The effects of p40 on HB-EGF release and ADAM17 activation in vivo are examined after administration of p40-containing pectin/zein hydrogel beads to mice. p40 stimulates ADAM17 activity and EGFR activation in colonic epithelial cells and increases HB-EGF levels in blood from WT mice, but not from mice with intestinal epithelial cell-specific ADAM17 deletion. Thus, these data define a mechanism of a probiotic-derived soluble protein in modulating intestinal epithelial cell homeostasis through ADAM17-mediated HB-EGF release, leading to transactivation of EGFR.

  7. Relative cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic Lactobacillus rhamnosus GG in managing infants with cow’s milk allergy in Spain

    Science.gov (United States)

    Guest, Julian F; Weidlich, Diana; Mascuñan Díaz, J Ignacio; Díaz, Juan J; Ojeda, Pedro Manuel; Ferrer-González, J Pablo; Gil, David; Onrubia, Isabel; Rincón Victor, Pedro

    2015-01-01

    Objective To estimate the cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic Lactobacillus rhamnosus GG (eHCF + LGG; Nutramigen LGG) as a first-line management for cow’s milk allergy compared with eHCF alone, and amino acid formulae in Spain, from the perspective of the Spanish National Health Service (SNS). Methods Decision modeling was used to estimate the probability of immunoglobulin E (IgE)-mediated and non–IgE-mediated allergic infants developing tolerance to cow’s milk by 18 months. The models also estimated the SNS cost (at 2012/2013 prices) of managing infants over 18 months after starting a formula as well as the relative cost-effectiveness of each of the formulae. Results The probability of developing tolerance to cow’s milk by 18 months was higher among infants with either IgE-mediated or non–IgE-mediated allergy who were fed eHCF + LGG compared with those fed one of the other formulae. The total health care cost of initially feeding infants with eHCF + LGG was less than that of feeding infants with one of the other formulae. Hence, eHCF + LGG affords the greatest value for money to the SNS for managing both IgE-mediated and non–IgE-mediated cow’s milk allergy. Conclusion Using eHCF + LGG instead of eHCF alone or amino acid formulae for first-line management of newly-diagnosed infants with cow’s milk allergy affords a cost-effective use of publicly funded resources because it improves outcome for less cost. A randomized controlled study showing faster tolerance development in children receiving a probiotic-containing formula is required before this conclusion can be confirmed. PMID:26648744

  8. Species Identification of the Lactobacillus casei Group by pheS Gene Sequences Analysis%pheS基因序列分析在干酪乳杆菌群种水平鉴定中的应用

    Institute of Scientific and Technical Information of China (English)

    刘光全; 刘勇; 李辉; 程池

    2011-01-01

    对千酪乳杆菌群(Lactobacillus casei group)内5个种和4个亚种的23个代表菌株的苯丙氨酰-tRNA合成酶a亚基基因(pheS)序列和18个16SrRNA基因序列进行系统发育学分析,探索pheS基因序列分析在干酪乳杆菌群种水平鉴定中应用的可行性。分析结果表明,pheS基因序列群内种间的差异率在6.8%~34%之间.种内最大差异率可达3%,其变异率远高于16SrRNA基因,可作为该群内种水平鉴定的重要手段。%The phenylalanyl-tRNA synthase alpha subunit (pheS) gene sequences and 16S rRNA gene sequences of representative strains of 5 species and 4 subspecies from Lactobacillus casei group were analyzed by phylogenic method. The feasibility of the pheS gene sequences for species identification of the Lactobacillus casei group was studied. Results showed that the interspecies divergence of pheS gene sequences was between 6.8% N 34% and the highest intra-species divergence could reach 3%. The discriminatory power ofpheS gene sequences was higher than that of 16S rRNA gene sequences, the pheS gene sequences analysis could be an important tool in species identification in the LactobaciUus casei group.

  9. Within-population variability in morphology and life history of Plantago major L. ssp. pleiosperma Pilger in relation to environmental heterogeneity

    NARCIS (Netherlands)

    Lotz, L.A.P.; Olff, H.; Tienderen, P.H. van

    1990-01-01

    An attempt was made to relate variation in life-history characteristics within a population of Plantago major ssp. pleiosperma to small-scale environmental variability. At a beach plain, embanked in 1966, a mosaic environment was distinguished with spatial variability in vegetation structure as well

  10. CITOGENETICS EFFECTS INDUCED BY THE ADMINISTRATION OF SUBSTANCES WITH REDUCTION POTENTIAL AT LARIX DECIDUA MILL. SSP. CARPATICA, PICEA ABIES (L. KARST. AND THUJA ORIENTALIS L

    Directory of Open Access Journals (Sweden)

    Ioana Anca Ieremia

    2007-08-01

    Full Text Available The paper present the influence of ascorbic acid, riboflavin, citric acid and sodium bisulphit upon the mitotic division of Larix decidua Mill ssp. carpatica, Picea abies (L. Karst and Thuja orientalis L. The treatment was applied in one variant, germinated seeds in ascorbic acid, riboflavin, citric acid and sodium bisulphit in 3 concentrations.

  11. The possible involvement of Mycobacterium avium ssp. paratuberculosis in the aetiology of Crohn's disease: a case control study in the Netherlands

    NARCIS (Netherlands)

    Herrewegh AAPM; Overduin P; Roholl PJM; Gielis FK; Robinson JE; Mahmmod N; Lieverse RJ; Robijn RJ; Zanden AGM van der; Soolingen D van; Dept of Medical Microbiology; Biomedics Scientific Consultancy; LIS; LPI

    2005-01-01

    A case control study was performed to investigate the possible role of Mycobacterium avium ssp. paratuberculosis (Map) in the aetiology of Crohn's disease (CD). Biopsy samples were collected from the ileum and colon of CD patients, Ulcerative Colitis (UC) patients and control persons. The biopsy sam

  12. Is there evidence for a link between Crohn's disease and exposure to Mycobacterium avium ssp. paratuberculosis? A review of current literature

    NARCIS (Netherlands)

    Herrewegh A; Roholl P; Overduin P; van der Giessen J; van Soolingen D; LIS; TOX; MGB

    2005-01-01

    Mycobacterium avium ssp. paratuberculosis (Map) wordt door veel onderzoekers beschouwd als mogelijke verwekker van de ziekte van Crohn (morbus Crohn, MC) bij de mens. Dit is vooral gebaseerd op klinische en pathologische overeenkomsten tussen MC en de ziekte Paratuberculose bij runderen (herkauwer

  13. The possible involvement of Mycobacterium avium ssp. paratuberculosis in the aetiology of Crohn's disease: a case control study in the Netherlands

    NARCIS (Netherlands)

    Herrewegh AAPM; Overduin P; Roholl PJM; Gielis FK; Robinson JE; Mahmmod N; Lieverse RJ; Robijn RJ; van der Zanden AGM; van Soolingen D; LIS; LPI

    2005-01-01

    De bacterie Mycobacterium avium ssp. paratuberculosis (Map) wordt beschouwd als een mogelijke oorzaak van de ziekte van Crohn (morbus Crohn, MC). In samenwerking met Gelre ziekenhuizen heeft het RIVM een onderzoek uitgevoerd naar het voorkomen van Map in darmbiopten van patienten met MC, patienten

  14. Manufacture of Fior di Latte cheese by incorporation of probiotic lactobacilli.

    Science.gov (United States)

    Minervini, F; Siragusa, S; Faccia, M; Dal Bello, F; Gobbetti, M; De Angelis, M

    2012-02-01

    This work aimed to select heat-resistant probiotic lactobacilli to be added to Fior di Latte (high-moisture cow milk Mozzarella) cheese. First, 18 probiotic strains belonging to Lactobacillus casei, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus reuteri were screened. Resistance to heating (65 or 55°C for 10 min) varied markedly between strains. Adaptation at 42°C for 10 min increased the heat resistance at 55°C for 10 min of all probiotic lactobacilli. Heat-adapted L. delbrueckii ssp. bulgaricus SP5 (decimal reduction time at 55°C of 227.4 min) and L. paracasei BGP1 (decimal reduction time at 55°C of 40.8 min) showed the highest survival under heat conditions that mimicked the stretching of the curd and were used for the manufacture of Fior di Latte cheese. Two technology options were chosen: chemical (addition of lactic acid to milk) or biological (Streptococcus thermophilus as starter culture) acidification with or without addition of probiotics. As determined by random amplified polymorphic DNA-PCR and 16S rRNA gene analyses, the cell density of L. delbrueckii ssp. bulgaricus SP5 and L. paracasei BGP1 in chemically or biologically acidified Fior di Latte cheese was approximately 8.0 log(10)cfu/g. Microbiological, compositional, biochemical, and sensory analyses (panel test by 30 untrained judges) showed that the use of L. delbrueckii ssp. bulgaricus SP5 and L. paracasei BGP1 enhanced flavor formation and shelf-life of Fior di Latte cheeses.

  15. Progeny of Eucalyptus globulus Labill ssp. Globulus%蓝桉子代测定

    Institute of Scientific and Technical Information of China (English)

    臧道群; 张川红; 罗军民; 黄永祥; 朱仁刚

    2003-01-01

    本文对云南省一平浪蓝桉实生种子园的子代测定林进行初期评价.该子代测定采用α-循环设计,21个重复,每个重复有18个不完全区组,每个区组分为15个小区,每小区2株树.全部270个家系均为自由授粉的半同胞家系,按来源分为3个类型(原产地天然林、云南地方品种和种子园),11个地区.调查和分析的数据包括造林后2年时的树高、胸径、冠幅、干形、抗寒性和存活率等性状.分析结果如下:1. 不同的类型在生长表现上存在显著差异,来自种子园的类型为最好.2. 自不同地区的蓝桉在各性状上都表现出显著差异.在生长表现上,来自维克多利亚东部、西部和各种子园的子代比其它地区的好.云南当地蓝桉子代的耐寒性和两年时的保存率为最好,在生长、干形和抗病方面与塔斯马尼亚东部和南部的蓝桉子代表现相似.采用两株小区,明显比单株小区降低小区缺失率,提高分析的可靠性.表3参7.%The preliminary assessment of progeny test in a seed orchard of Eucalyptus globulus Labill. ssp. globulus at Yipinglang State Farm of Yunnan Province in China was conducted. The trial was composed of 21 replications, with 2 trees on each plot, 15 plots in each incomplete block, and 18 incomplete blocks in each replication, laid out in a α- design of 270 open-pollinated families . The families were of three categories (natural populations, local land race and seed orchard). They were further subdivided into 11 regions. The data from an assessment of growth, stem form and other characteristics of the young trees after being planted 2 years were analyzed. Results are as follows: the different categories differed significantly in their growth, with the families from seed orchards being the best. Regions also differed significantly in their growth. The families from the regions of Western Victoria, Eastern Victoria and all three seed orchards were better than others. The

  16. Evaluación de la fermentación del lactosuero ácido (entero y desproteinizado utilizando Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Katia Cury Regino

    2014-06-01

    Full Text Available Título corto: Evaluación de la fermentación del lactosuero ácidoTítulo en ingles: Evaluation of acid whey fermentation (whole and deproteinized using Lactobacillus caseiResumen:  El lactosuero acidificado espontáneamente pone en riesgo la salud del consumidor al utilizarlo como acidificante de la leche usada en la elaboración del queso tipo mozzarella, y además reduce la calidad del producto. El objetivo de esta investigación fue evaluar el proceso de fermentación del lactosuero ácido (entero y desproteinizado con Lactobacillus casei, utilizando diferentes porcentajes de su inóculo. Se caracterizaron fisicoquímicamente los lactosueros, sometiéndolos a fermentación anaeróbica a 37°C y 120 rpm por 96 h. Se evaluó la concentración de biomasa celular, el consumo de lactosa, la producción de ácido láctico (AL y se estimaron los parámetros cinéticos y estequiométricos. Se aplicó un diseño experimental completamente al azar con arreglo factorial 2x3, empleando la metodología de medidas repetidas. Los datos, se procesaron a través de los paquetes estadísticos SAS® versión 9 y Statgraphics Centurión XVI®. El lactosuero, entero y desproteinizado, se encontró dentro de los intervalos fisicoquímicos aceptables para su acidificación. Los valores de acidez fueron superiores a 120 Grados Dornic (120°D con la inoculación  de L. casei, excepto en lactosuero desproteinizado, inoculado con 15% de cultivo. El lactosuero entero con 15% de inóculo, alcanzo 120°D en el menor tiempo (34h. Altas concentraciones de inóculo (15% favorecieron la acidificación en el lactosuero entero, mostrando la mayor producción (20.83gAL/l, Y´p/s=0.86, Qp=0.173gAL/l*h; mientras el lactosuero desproteinizado mostró un mayor crecimiento microbiano, menor conversión de lactosa (8.2% y menor producción media de ácido láctico (8.19g/l.Palabras clave: suero lácteo; ácido láctico; queso hilado;  parámetros cin

  17. Enhanced natural killer cell activation by exopolysaccharides derived from yogurt fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1.

    Science.gov (United States)

    Makino, Seiya; Sato, Asako; Goto, Ayako; Nakamura, Marie; Ogawa, Miho; Chiba, Yoshika; Hemmi, Jun; Kano, Hiroshi; Takeda, Kazuyoshi; Okumura, Ko; Asami, Yukio

    2016-02-01

    Yogurt is generally recognized as a beneficial food for our health, but research into its physiological effects has focused mainly on intestinal dysfunctions such as constipation and diarrhea. We previously found yogurt fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (hereafter OLL1073R-1) could reduce risks of catching the common cold and flu in human trials. It was assumed that immunostimulatory exopolysaccharide (EPS) produced from OLL1073R-1 play an important role in this context. However, few studies have examined the immunostimulatory effects of traditional Bulgarian yogurts fermented with different strains of lactobacilli and their metabolites. Therefore, we screened 139 L. delbrueckii ssp. bulgaricus strains and identified OLL1073R-1 as the most robust producer of EPS. This strain was also the only strain that induced the production of IFN-γ in vitro. Oral administration of the EPS or yogurt fermented with OLL1073R-1 and Streptococcus thermophilus OLS3059 (OLL1073R-1 yogurt) augmented natural killer (NK) cell activity and induced IFN-γ production in spleen cells in mice, whereas 2 other yogurts fermented with other strains had no effect on NK cell activity. Cellular preparations of the OLL1073R-1 strain also slightly augmented NK cell activity, but were less effective than EPS itself. The EPS-dependent stimulation of NK cell activity was abrogated in IFN-γ knockout mice and in myeloid differentiation factor 88 knockout mice. Furthermore, IFN-γ production from spleen cells stimulated with EPS was completely blocked with both anti-IL-12 and anti-IL-18 antibodies in vitro. These findings suggest that NK cell activation by OLL1073R-1 yogurt is EPS-dependent, occurs via IL-12- and IL-18-mediated IFN-γ production, and requires myeloid differentiation factor 88. We showed that traditional Bulgarian yogurt could exert immunostimulatory effects by selecting starter strains and part of the mechanisms depend on IFN-γ inducible EPS produced

  18. Primula munroi 的产自东喜马拉雅的一个新亚种 --P. munroi ssp. schizocalyx%A new subspecies of Primula munroi Lindl. from the Eastern Himalaya--P. munroi ssp. schizocalyx

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ In the course of revising the genus Primula Linn.(Primulaceae) in the Himalayas of Indian region, the authors came across a set of collections identified as P. involucrata Wall. gathered by the pioneer Himalayan travellers, merely written on determinavit slips as P. involucrata Wall. forma schizocalyx by I. B. Balfour without any date. Critical studies revealed that these specimens are quite distinct from the species proper so far described. The new taxon is described as a subspecies and illustrated. As P. involucrata Wall. ex Duby (1844) is a later homonym of P. involucrata Sweet (1839) and illegitimate, and its correct name is P. munroi Lindl. (1847), we name our new subspecies as P. munroi ssp. schizocalyx Balf. f. ex Basak et Maiti. 

  19. Pólen 2n e mecanismos meióticos de formação em Solanum commersonii ssp 2n pollen and meiotic mechanisms of Formation in Solanum commersonii ssp

    Directory of Open Access Journals (Sweden)

    Lívia Gracielle Oliveira Tomé

    2009-04-01

    Full Text Available Solanum commersonii subsp. commersonii Dun. e Solanum commersonii subsp. malmeanum Bitt. (2n=2x=24 - 1EBN não podem ser diretamente cruzadas com a batata cultivada de S. tuberosum subsp. tuberosum L. (2n=4x=48 -4 EBN em razão da barreira da ploidia e do número de equilíbrio do endosperma (EBN. No entanto, a produção de pólen não reduzido por S. commersonii ssp. (2x=2n=24 - 1EBN, permite o cruzamento desta com dihaplóides de batata e com as espécies diplóides S. phureja Juz. & Bukasov. e S. chacoense Bitt., que possuem 2 EBN. Conduziu-se este trabalho, com o objetivo de determinar o mecanismo meiótico e a freqüência dos grãos de pólen não reduzidos em cinco clones de Solanum commersonii ssp. Foram avaliados três clones de S. commersonii subsp. commersonii (SCC e dois de S. commersonii subsp. malmeanum (SCM. Nas avaliações, duzentos grãos de pólen viáveis foram corados (carmim acético 2% e mensurados, sendo considerados não reduzidos aqueles com o diâmetro igual ou superior a 25 µm. O clone Solanum commersonii subsp. commersonii Dun. (SCC 100 produz 26.1% de polen não reduzido, por fusos fundidos e fusos paralelos, o qual pode ser utilizado em programas de melhoramento.Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. (2n=2x=24 -1EBN cannot be directly crossed with the cultivated Solanum tuberosum subsp. Tuberosum L. (2n=4x=48 - 4EBN species due to ploidy barriers and endosperm balance number (EBN. However, non-reduced pollen production by the wild species S. commersonii ssp. (2x=2n=24 -1EBN, allows the crossing of these species with dihaploids of potato and with the diploids species, S. phureja Juz. & Bukasov. and S. chacoense Bitt., all with 2EBN. The aim of this work was to determine the meiotic mechanism and frequency of non-reduced pollen production of five clones of S. commersonii ssp. Three different clones of S. commersonii subsp. commersonii Dun. (SCC and two of S

  20. EVALUACIÓN DE UNA COLECCIÓN DEL GÉNERO Triticum: TRIGO HARINERO (Triticum aestivum ssp aestivum, TRIGO DURO (Triticum turgidum ssp. durum Y TRITICALE (X Triticum secale Wittmack EN LAS CONDICIONES DEL OCCIDENTE DE CUBA

    Directory of Open Access Journals (Sweden)

    R. Plana

    2006-01-01

    Full Text Available En el Instituto Nacional de Ciencias Agrícolas (INCA se evaluó una colección del género Triticum, con el objetivo de conocer el comportamiento de diferentes especies en las condiciones del occidente de Cuba. Las especies estudiadas fueron: Triticum aestivum ssp. aestivum, Triticum turgidum ssp. durum y X Triticum secale Wittmack. Se utilizó un diseño experimental de bloques al azar con cuatro repeticiones. Los caracteres analizados fueron altura de la planta (cm, longitud de las espigas (cm, masa de mil granos (g y rendimiento agrícola (t.ha-1. Se utilizó la estadística descriptiva para cada una de las variables estudiadas, estimándose la media y desviación típica por especie. La variedad cubana de trigo harinero CC-204 fue utilizada como control. La matriz de datos obtenida (genotipo x variable fue procesada mediante la técnica multivariada de Componentes Principales. Los datos para todos los análisis estadísticos se ejecutaron por el paquete estadístico SPSS 11. 0 para Windows. La mayoría de los genotipos tuvieron un comportamiento medio general, aunque se separaron del resto algunos genotipos de trigo harinero y triticale. Los valores de la media y desviación estándar para las variables analizadas muestran que no fue posible distinguir diferencias por especie, aunque las plantas de trigo duro fueron las de menor altura, siendo los mejores genotipos: 12 y 54 de la especie T. aestivum (trigo harinero; 17, 18 y 25 de T. durum (trigo duro; 29 y 37 del X Triticum secale (tritic