WorldWideScience

Sample records for cascavel sul-americana crotalus

  1. Epidemiologia e quadro clínico do acidente por cascavel sul-americana (Crotalus durissus Epidemiology and clinical features of South American rattlesnakes (Crotalus durissus envenomation

    Directory of Open Access Journals (Sweden)

    Miguel Tanús Jorge

    1992-08-01

    Full Text Available Foram estudados 249 casos de acidentes por cascavel sul-americana (Crotalus durissus atendidos no HVB-IB, São Paulo, Brasil, de 1974 a 1990. Os acidentes foram mais comuns no período da tarde, nos meses de janeiro a abril e de outubro a dezembro. Dentre cem serpentes classificadas quanto à subespécie 99 eram C. d. terrificus. Pertenciam ao sexo masculino 80,7% dos pacientes. Os membros inferiores e superiores foram picados em, respectivamente, 66,4% e 29,2% dos casos. As manifestações clínicas mais freqüentes foram dor (61,0% e edema (55,0% no local da picada, ptose palpebral (75,9%, escurecimento da urina atribuível à mioglobinúria (38,6% e mialgia (36,1%. Nove pacientes foram submetidos a diálise devido a insuficiência renal aguda (3,6%, três apresentavam insuficiência respiratória que motivou intubação e/ou traqueostomia e um apresentou acidente vascular cerebral isquêmico. A alteração da coagulação sanguínea ocorreu em 48,1% dos pacientes. Oito amostras de sangue colhidas horas após a picada mostraram leucocitose sendo 6 com desvio a esquerda e, nos dias subseqüentes, tendência à normalização do número de leucócitos e aparecimento de eosinofilia. Atividade sérica da creatinoquinase apresentou-se aumentada em 20 dentre 21 pacientes, sendo maior no final das primeiras 24 horas após a picada, chegando a 2.377 vezes o valor de referência. A letalidade foi de 0,8%.Under study were two hundred and forty nine cases of accidents caused by South American rattlesnakes (Crotalus durissus admitted to the Vital Brasil Hospital, São Paulo, Brasil, from 1974 to 1990. The accidents were more common in the afternoon, from January to April and from October to December. Ninety nine out of 100 snakes classified as subspecies were C. d. terrificus. Most of the patients were males (80.7%. The inferior and superior limbs were bitten in 66.4% and 29.2% of the cases, respectively. The more frequent clinical manifestations were

  2. Transferência do Hepatozoon tupinambis, parasita do lagarto Tupinambis teguixin, para a serpente cascavel (Crotalus durissus terrificus, por intermédio do mosquito Culex fatigans

    Directory of Open Access Journals (Sweden)

    Samuel B. Pessôa

    1974-01-01

    Full Text Available Os autores obtiveram a transferência do Hepatozoon tupinambis (Laveran e Salibeni, 1909 parasita do lagarto Teiidae, Tupinambis teguixin, L., para a serpente cascavel, Crotalus durissus terrificus (Laur., alimentando-a com mosquitos experimentalmente infectados. o parasita mantém os seus caracteres morfológicos no animal receptor, nos limites do tempo observado (cerca de 100 dias. O ofídio receptor apresentou cistos esquizogônicos do fígado.The authors achieved a transfer of the Hepatozoon tupinambis (Laveran and Salibeni, 1909, a parasite of the Sauria: Tupinambis teguixin, L., to a rattlesnake of the species Crotalus durissus terrificus, feeding the latter with experimentally infected mosquitoes. The parasite maintained its morphological characteristics in the recepting animal throughout the whole observation period (about 100 days. Schizogonic cysts were found in the liver of the rattlesnake.

  3. Perfil eletroforético das proteínas séricas de serpentes Crotalus durissus terrificus (cascavel criadas em cativeiro Serum protein electrophoresis profile of the rattlesnake Crotalus durissus terrificus kept in captivity

    Directory of Open Access Journals (Sweden)

    Joandes Henrique Fonteque

    2009-06-01

    Full Text Available As serpentes peçonhentas dos gêneros Bothrops e Crotalus têm sido mantidas em cativeiro visando à extração de venenos para a produção de imunobiológicos. O conhecimento da fisiologia desses animais e as alterações na concentração de proteínas e suas frações séricas são importantes para a identificação precoce de importantes enfermidades que cursam com estados de hipoproteinemia e hiperproteinemia. O objetivo do trabalho foi determinar a concentração de proteína total e o perfil eletroforético das proteínas séricas de serpentes Crotalus durissus terrificus (cascavel criadas em cativeiro. Foram colhidas amostras de sangue da veia coccígea ventral de 21 serpentes adultas e sadias, divididas em dois grupos: Grupo 1 de 12 machos com peso médio de 588,89±193,55g, e Grupo 2 de nove fêmeas com peso médio de 708,33±194,04g. A proteína total sérica foi determinada pelo método de refratometria e a eletroforese em gel de agarose. Obtiveram-se valores da proteína total sérica (g/dL de 4,51±0,50 para machos e de 4,82±0,72 para fêmeas, e para machos e fêmeas de 4,64±0,61. Foram identificadas pela eletroforese quatro frações protéicas (g/dL: albumina, a, b, g-globulinas e calculada a relação albumina:globulina. As serpentes fêmeas apresentaram maiores valores para as variáveis, albumina e para a relação albumina/globulina (AG diferindo significativamente (PThe poisonous snakes of the genera Crotalus and Bothrops have been kept in captivity with the purpose of extracting poison for the production of immunobiological. Knowledge of the physiology of these animals and serum proteins concentration changes are important for early identification of major diseases which lead to states of hypoproteinemia and hyperproteinemia. The objective was to determine the concentration of total protein and serum protein electrophoresis profile of Crotalus durissus terrificus (rattlesnake in captivity. Blood samples were taken from

  4. A Comunidade Sul-Americana de Nações no contexto da política externa do Brasil

    Directory of Open Access Journals (Sweden)

    Carlos Ribeiro Santana

    2011-02-01

    Full Text Available O presente artigo tem como finalidade realizar um balanço sobre o significado da I Reunião de Chefes de Estado da Comunidade Sul-Americana de Nações, no que se refere à política externa.

  5. Análise institucionalista da integração sul-americana = Institutionalist analysis of South American integration

    Directory of Open Access Journals (Sweden)

    Mallmann, Maria Izabel

    2010-01-01

    Full Text Available A integração sul-americana é aqui identificada pelos compromissos regionais assumidos ao longo da década de 1990; lançados durante a 7ª Cúpula do Grupo do Rio, de outubro de 1993, seguidos pelas Cúpulas de presidentes da América do Sul (2000, 2002, 2004, pela criação da Iniciativa para a Integração da Infraestrutura Regional Sul-Americana (IIRSA, em 2000, da Comunidade Sul-Americana das Nações (Casa, em 2005 e da União das Nações Sul-Americanas (Unasul, em 2007. Analisa-se o processo tendo como parâmetro as condições e mecanismos apontados por estudos institucionalistas como essenciais para o sucesso de um processo de integração como, por exemplo, a simetria regional e a estabilidade interna, dentre outras. Constata-se que essas duas são as condições mais problemáticas no caso sul-americano, uma vez que interpõem obstáculos ao avanço nas demais. Um balanço do estudo realizado até aqui permite estimar que o processo de integração tende a ser mais longo e errático do que seria desejável, uma vez que se encontra no que poderia ser entendida como fase inicial de implementação de mecanismos e de geração das condições básicas necessárias

  6. Localização nervosa da blastomicose sul-americana

    Directory of Open Access Journals (Sweden)

    Açucena Raphael

    1966-06-01

    Full Text Available Foram estudados sob os pontos de vista clínico-neurológico e liquorológico, 55 doentes que apresentavam formas variadas, tegumentares e viscerais, da blastomicose sul-americana; no seguimento, 46 dêsses casos foram submetidos a nôvo exame clínico-neurológico e 30 foram repuncionados para novos exames de LCR. Dos 55 pacientes estudados, 14 apresentavam sintomas ou sinais clínicos sugestivos de comprometimento do SNC e/ou de seus envoltórios; em 5 casos deste último grupo o exame do líquido cefalorraqueano forneceu dados que permitiam o diagnóstico etiológico. Dos 41 doentes que não apresentavam sinais ou alterações neurológicas, em um o exame do LCR forneceu dados de valor diagnóstico, inclusive a positividade da pesquisa micológica. Vinte e três doentes foram considerados normais do ponto de vista neurológico e 26 mostraram alterações clínicas e liqüóricas ocasionais e duvidosas quanto ao seu valor diagnóstico. De acôrdo com os resultados obtidos pelo exame do LCR, os doentes foram distribuídos em 3 grupos: 1 doentes com LCR normal; 2 doentes cujo LCR apresentava um conjunto de alterações atribuíveis à paracoccidioidose; 3 doentes cujo LCR apresentava alterações classificáveis como ocasionais ou duvidosas quanto ao seu valor para o diagnóstico etiológico. As alterações do LCR consideradas como sugestivas quanto ao diagnóstico etiológico consistiram em: a modificações quantitativas e qualitativas das frações protêicas, sendo mais freqüente o aumento do teor de gama-globulina; b positividade da reação de fixação do complemento com título acima de 3; c pleocitose, intensa e do tipo misto mas com predominância de linfócitos nos casos em que havia acometimento leptomeníngeo, discreta e de tipo linfomononuclear nos casos de provável localização parenquimatosa; d hipoglicorraquia que ocorreu apenas nos casos de localização leptomeníngea; e positividade do exame micológico. A presença de

  7. Aspectos clínico-patológicos e laboratoriais do envenenamento crotálico experimental em bovinos Clinical and pathological aspects of experimental Crotalus poisoning in cattle

    Directory of Open Access Journals (Sweden)

    Flávio A.S. Graça

    2008-06-01

    Full Text Available Reproduziu-se experimentalmente o envenenamento crotálico, através da inoculação, por via subcutânea, do veneno de Crotalus durissus terrificus (cascavel sul-americana em dez bovinos mestiços. Dois animais foram utilizados como controle. O bovino que recebeu dose de 0,03mg/kg de peso corporal, morreu 7h40min após a inoculação. A dose de 0,015mg/kg causou a morte em quatro de sete bovinos inoculados, enquanto os dois animais que receberam 0,0075mg/kg adoeceram discretamente e se recuperaram. Os sintomas tiveram início entre 1h30min e 13h45min após a inoculação. A evolução oscilou entre 5h25min e 45h para os animais que morreram e entre 33h15min e 17 dias entre os animais que se recuperaram. Os principais sinais nervosos observados foram diminuição da resposta aos estímulos externos, reflexos hipotônicos, arrastar dos cascos no solo, aparente apatia, paralisia do globo ocular e da língua, decúbito esternal e lateral. Verificaram-se também adipsia e, por vezes, petéquias nas mucosas vaginal e conjuntival. Houve discreto a moderado aumento do tempo de sangramento e moderado aumento do tempo de tromboplastina parcial ativada. Houve moderada leucocitose com neutrofilia, linfopenia relativa, eosinopenia, monocitose e discreto aumento do número de bastões. Foi evidenciado significativo aumento dos níveis séricos de creatinaquinase, contudo, não foram observadas alterações significativas através da urinálise. À necropsia constataram-se edema quase imperceptível no local da inoculação, discretas petéquias e sufusões no epicárdio, omento, vesícula biliar e mucosa da bexiga em alguns dos animais envenenados experimentalmente. Os exames histopatológicos revelaram necrose (hialinização de grupos de miócitos ou em miócitos isolados em dez diferentes músculos esqueléticos examinados, próximos ou distantes do local de inoculação em todos os animais necropsiados. Concluí-se que o envenenamento por Crotalus Sul-americanas

  8. Evaluation of contraction in isolated corpus cavernosum from Crotalus durissus terrificus

    OpenAIRE

    Renata Lopes Rodrigues

    2013-01-01

    Resumo: O fato da cópula em serpentes ser muito prolongada, chegando a mais de 20 horas, sem que os mecanismos farmacológicos estejam conhecidos, motivou o nosso grupo a estudar o hemipênis de Crotalus durissus terrificus (cascavel). Diferentemente do observado em corpo cavernoso de outros mamíferos, o relaxamento induzido pela estimulação elétrica foi mais duradouro, além de resistente a ação da neurotoxina tetrodotoxina (TTX) em hemipênis de Crotalus. Uma hipótese inicial aventada foi que e...

  9. Emprego da associação sulfametoxazol + trimetoprim no tratamento da paracoccidioidose (blastomicose Sul-Americana

    Directory of Open Access Journals (Sweden)

    P. N. Pedrosa

    1974-06-01

    Full Text Available Acentuando a necessidade da continuação das pesquisas terapêuticas em pacientes com paracoccidioidose (blastomicose sul-americana, os autores apresentam 23 pacientes submetidos a diferentes esquemas terapêuticos, sendo 14 virgens de tratamento e 9 com uso prévio de uma ou mais drogas. A associação sulfametoxazol + trimetoprim ( SMZ + TMP foi empregada em 5 pacientes virgens de tratamento e 9 sulfa-resistentes. Outros sete foram submetidos ao esquema clássico com sulfadoxina. Os pacientes que não responderam aos dois esquemas anteriores, com exceção de dois casos inicialmente graves, receberam anfotericina B. A avaliação clínica, radiológica, micológica e sorológica a longo prazo não demonstrou vantagens no emprego de SMZ + TMP em substituição aos sulfamídicos, nos pacientes virgens de tratamento. Entretanto, a associação SMZ + TMP parece ser uma opção válida nos casos sulfa-resistentes, onde teria primazia, considerando-se a toxicidade e necessidade de controle em regime hospitalar da anfotericina B. Ressaltam ainda a boa tolerância clínica e laboratorial da associação SMZ + TMP em cursos terapêuticos prolongados de até 2 anos, quando empregadas em baixas doses de manutenção.

  10. Dietas artificiais para a criação de larvas e adultos da mosca-das-frutas sul-americana

    Directory of Open Access Journals (Sweden)

    Adrise Medeiros Nunes

    2013-10-01

    Full Text Available O objetivo deste trabalho foi adequar as dietas artificiais para o desenvolvimento dos estágios de larva e adulto da mosca-das-frutas sul-americana (Anastrepha fraterculus. Para o estágio larval, foram testadas as seguintes dietas: D1, original, com 10 g de ágar; D2, modificada, com 3,6 g de ágar; e, D3, modificada, com bagaço seco de cana-de-açúcar. Para os adultos, foram testadas quatro dietas: A, levedura de cerveja + mel (2:1; B, açúcar refinado + extrato de levedura + gérmen de trigo cru (3:1:1; C, extrato de soja + açúcar mascavo + gérmen de trigo cru (3:1:1; e D, levedura seca de cervejaria + mel (2:1. Avaliaram-se os parâmetros biológicos de duração do período ovo-pupa, duração e viabilidade do estágio de pupa, massa média de pupas, razão sexual e duração e viabilidade do período ovo-adulto. O desenvolvimento larval em D1 e D2 foi semelhante e indicou que a criação de larvas pode ser realizada com 1/3 da quantidade de ágar da utilizada em D1. A utilização do bagaço seco de cana-de-açúcar, na dieta artificial, afetou negativamente o desenvolvimento larval. As dietas artificiais com levedura de cerveja + mel e com açúcar refinado + extrato de levedura + gérmen de trigo cru são as mais adequadas para a criação de adultos.

  11. Localização encefálica da blastomicose sul-americana. Considerações a propósito de 9 casos

    Directory of Open Access Journals (Sweden)

    Walter C. Pereira

    1965-06-01

    Full Text Available Lesões encefálicas produzidas pelo Paracoccidioides brasiliensis têm sido registradas na literatura com relativa freqüência. Os autores chamam a atenção para a dificuldade diagnóstica dêstes processos que, na quase totali- dade, constituem-se de achados cirúrgicos ou de necropsia. A síndrome de hipertensão intracraniana é a manifestação mais comum, levando quase sempre ao diagnóstico de tumor intracraniano. Os exames complementares, inclusive o líquido cefalorraqueano, são pobres em dados específicos; o encontro do parasita, assim como a positividade das reações de fixação de complemento e de precipitação nesse humor, são raramente verificadas. A natureza da afecção só tem sido suspeitada quando existem lesões paracocci-dióicas conhecidas em outros órgãos. Nove casos de blastomicose sul-americana com localização encefálica são estudados. Sete foram submetidos a intervenções cirúrgicas, dêstes, 3 tiveram boa evolução, sendo os únicos que sobreviveram de tôda a série. Nos três casos foi empregada, em épocas diversas depois da intervenção cirúrgica, anfotericina B pelas vias intravenosa ou intratecal. Os autores consideram a excição cirúrgica dos granulomas encefálicos imprescindível, mormente quando existem sinais de compressão bem localizadas. Após o ato cirúrgico a administração de anfotericina B, pelas vias intravenosa ou intratecal, deve ser prescrita a fim de evitar possíveis recidivas da moléstia.

  12. Projeto "Viver em Cascavel": análise do fluxo de informações Proyecto "Vivir en Cascavel": análisis del flujo de informaciones Project "To live in Cascavel": analysis of the information flow

    Directory of Open Access Journals (Sweden)

    Nelsi Salete Tonini Paiva

    2002-07-01

    Full Text Available O presente estudo estimou e analisou o tempo (em dias do fluxo das Declarações de Nascidos Vivos (DNV de risco atendidos no Projeto "Viver em Cascavel"- Paraná, 1996 a 1998, segundo os espaços organizativos (hospital, vigilância epidemiológica, unidade básica de saúde. Foram analisadas 303 declarações. Para análise do tempo de fluxo da DNV, foram construídos intervalos de 95% de confiança para os tempos médios populacionais (em dias. Os resultados apontam que a DNV demorou para fluir do hospital até o momento de realização da visita domiciliária, de 25 a 30 dias. A atenção ao recém nascido de risco deve acontecer nos prazos mais breves possíveis. Nesse sentido, o tempo de fluxo da DNV encontrado neste estudo, do nascimento à visita domiciliária, pode comprometer um dos objetivos do Projeto em questão, qual seja, a redução da morbimortalidade infantil no município.El presente estudio estimó y analizó el tiempo (en días de flujo de los certificados de nacimiento de niños con riesgo, atendidos en el Proyecto "Vivir en Cascavel" - Paraná, 1996 hasta 1998, según los espacios organizacionales (hospital, vigilancia epidemiológica, unidad básica de salud. Fueron analizados 303 certificados. Para el análisis del tiempo de flujo del certificado fueron construidos intervalos de 95% de confianza para los tiempos medios poblacionales (en días. Los resultados muestran que el tiempo promedio de flujo es largo, entre 25 y 30 días. La atención al recién nacido de riesgo debe acontecer en plazos lo más breves posibles y, en este sentido, el tiempo de flujo del certificado, desde el nacimiento hasta la visita domiciliaria, puede estar comprometiendo uno de los objetivos del proyecto analizado, o sea, la reducción de la morbi-mortalidad infantil en el municipio.This study estimated and evaluated the time period (in days concerning the flow of Live Birth Declarations (DNV for high-risk newborns assisted by the Project

  13. Avaliação do Centro de Atenção Psicossocial Infantil de Cascavel - PR Evaluation of the Child Psychosocial Care Center of Cascavel - PR

    Directory of Open Access Journals (Sweden)

    Ana Silvia Scandolara

    2009-12-01

    Full Text Available Estudo relacionado à avaliação de serviços em saúde mental, neste caso, o Centro de Atenção Psicossocial Infantil - CAPSi, do município de Cascavel-PR, serviço substitutivo implementado a partir da transformação da assistência psiquiátrica no Brasil. Objetivou avaliar qualitativamente o CAPSi. Os participantes foram 10 profissionais de saúde que atuam no CAPSi, sendo que os dados foram analisados sob a técnica do Discurso do Sujeito Coletivo (DSC (Lefèvre & Lefèvre, 2003. Como resultado, construíram-se 06 DSC e destacaram-se 12 temas geradores de significações: bom atendimento, estrutura física, recursos humanos, rede de serviços, acesso, referência, organização do serviço, família, atende as políticas de saúde mental, demanda, capacitação, redefinição de serviços, qualidade do serviço, papel do CAPSi e rotatividade de recursos humanos. Com base nos DSC o atendimento prestado no CAPSi é de boa qualidade, porém existem dificuldades de estrutura administrativa.This study is related to the evaluation of mental health services, in this case, the Child Psychosocial Care Center - (Centro de Atenção Psicossocial Infantil - CAPSi, in the city of Cascavel-PR, that is a substitute service implemented from the transformation of the psychiatric assistance in Brazil. It objectified to evaluate CAPSi qualitatively. The participants have been 10 health professionals who act in CAPSi, and the data has been analyzed under the technique of the General Subject Speech ( Discurso do Sujeito Coletivo - DSC (Lefèvre & Lefèvre, 2003. As result, 06 DSC have been constructed and 12 meaning-generating themes have been distinguished: good attendance, physical structure, human resources, net of services, access, reference, organization of the service, family, respect for the mental health policies, demand, qualification, redefinition of services, quality of the service, role of the CAPSi and rotation of human resources. Based on the

  14. Afibrinogenemia following snake bite (Crotalus durissus terrificus

    Directory of Open Access Journals (Sweden)

    C. F. S. Amaral

    1988-08-01

    Full Text Available This paper reports two cases of afibrinogenemia with normal platelet count following Crotalus durissus terrificus, snake bite Both patients presented high output acute renal failure and case two also had increased blood levels of CPK and LDH compatible with the diagnosis of rhabdomyolysis. Case one was given an unknown amount of antivenom and was treated with epsilonaminocaproic acid and a fresh whole blood transfusion and showed recovery of the coagulation disturbance 40 hours following these measures. Case two was given an adequate amount of crotalide antivenom and the coagulation tests performed 12 hours later showed a normal partial thromboplastin time and fibrinogen 86 mg/100ml. Case one presented no haemorrhagic disturbances. Case two presented persistent bleeding following venopuncture and after removal of impetigo crust in the legs. Acute renal failure was treated conservatively and both patients were discharged from the hospital with recovery of the renal function.

  15. Displasia broncopulmonar: incidência, fatores de risco e utilização de recursos em uma população sul-americana de recém-nascidos de muito baixo peso Bronchopulmonary dysplasia: incidence, risk factors and resource utilization in a population of South-American very low birth weight infants

    Directory of Open Access Journals (Sweden)

    Jose L. Tapia

    2006-02-01

    Full Text Available OBJETIVO: Determinar a incidência de displasia broncopulmonar, os fatores de risco e a utilização de recursos em uma ampla população sul-americana de recém-nascidos de muito baixo peso ao nascer MÉTODOS: Dados prospectivamente registrados de crianças com peso ao nascer entre 500 a 1.500 g, nascidas em 16 centros neonatais pertencendo à rede NEOCOSUR entre 10/2000 a 12/2003. A análise multivariada de Poisson com variância robusta foi utilizada para determinar os fatores de risco relativo e intervalo de confiança de 95% que afetam o risco de apresentação de displasia broncopulmonar RESULTADOS: Foram analisados 1.825 recém-nascidos de muito baixo peso ao nascer. As médias de peso ao nascer e a idade gestacional foram de 1.085±279g e 29±3 semanas, respectivamente. A incidência de displasia broncopulmonar foi de 24,4%, e a sobrevida sem displasia broncopulmonar aumentou quanto maior foi a idade gestacional. Maior peso ao nascer, maior idade gestacional e sexo feminino estiveram associados a um menor risco de displasia broncopulmonar. Aumentaram o risco de displasia broncopulmonar: ventilação mecânica, necessidade de surfactante, escape aéreo, persistência do canal arterial, sepse tardia e enterocolite necrotizante. As crianças com displasia broncopulmonar requerem um maior tempo hospitalização (91±27 versus 51±19, de ventilação mecânica (19±20 versus 4±7 e de oxigenioterapia (72±30 versus 8±14. CONCLUSÕES: A incidência de displasia broncopulmonar foi de 24,4% em uma ampla população sul-americana e se relaciona com uma maior utilização de recursos. Os fatores de risco associados à displasia broncopulmonar encontrados nesse estudo foram: ventilação mecânica, necessidade de surfactante, escape aéreo, persistência do canal arterial, sepse tardia e enterocolite necrotizante. As informações contidas neste estudo podem ser úteis para o delineamento de estratégias perinatais de prevenção da morbidade

  16. Irradiação global, direta e difusa, para a região de Cascavel, Estado do Paraná = Global, direct and diffuse irradiation in the region of Cascavel, Paraná State

    Directory of Open Access Journals (Sweden)

    Patricia Aparecida de Oliveira Drechmer

    2006-01-01

    Full Text Available O presente trabalho apresenta um estudo sobre os níveis das irradiações global, direta e difusa incidentes na superfície terrestre, a irradiação incidente no topo da atmosfera e a insolação relativa mensal, para a cidade de Cascavel-PR. As componentes da radiação solar foram monitoradas entre janeiro de 2001 e fevereiro de 2003 na Estação Experimental Agrometeorológica da Unioeste, Cascavel, Estado do Paraná. Na medição da radiação global foi utilizado um piranômetro KIPP & ZONEN-CM3 e para medir a componente direta foiutilizado um pireliômetro EPPLEY-NIP acoplado a um rastreador solar. A radiação difusa foi calculada pela diferença entre as radiações global e direta. Os níveis médios mensais das irradiações global, direta e difusa, foram respectivamente, 51,8%, 31,7% e 20,17% dairradiação incidente no topo da atmosfera. O índice de insolação relativa apresentou valor máximo em agosto (61,43% e mínimo em fevereiro (34,35%, períodos caracterizados respectivamente por dias de céu limpo e nublados no hemisfério sul.This paper reports a study about the diffuse, direct and global radiation levels on surface of earth, the irradiation over the top of atmosphere and the monthly relative sunstroke in the city of Cascavel, State of Paraná, southern Brazil. The solar radiation components were monitored from January, 2001 to February, 2003 at Estação Experimental Agrometeorológica of Unioeste, Cascavel, Parana State. Global radiation was monitored by a pyranometer KIPP & ZONEN-CM3 and direct radiation was monitored by a pyrheliometer EPPLEY-NIP coupled to a solar tracer. The diffuse radiation wasmonitored through the difference between global and direct radiation. Monthly average levels of global, direct and diffuse irradiations were 51.8%, 31.7% and 20.17% of the irradiation in the top of atmosphere. The relative isolation index showed the maximum value in August (61.43% and the minimum in February (34.35%. These

  17. Atendimento de crianças realizado pelo SIATE de Cascavel no ano de 2004

    Directory of Open Access Journals (Sweden)

    Márcio Briccius

    2008-03-01

    Full Text Available Os acidentes com crianças vêm-se constituindo, cada vez mais, em uma relevante causa de morbimortalidade. As injúrias físicas respondem por 98% das causas de mortes nos países em desenvolvimento. O estudo teve como objetivo, identificar os tipos de acidentes envolvendo crianças de zero a doze anos atendidas pelo SIATE de Cascavel. Trata-se de estudo descritivo, desenvolvido com a consulta realizada aos arquivos do SIATE, sobre os atendimentos realizado à crianças no ano de 2004. Obteve-se como resultado o registro de 379 atendimentos envolvendo crianças. As principais causas foram os acidentes de trânsito (63,6% e a queda (20,3%, atingindo meninos (63,6% com idade entre 10 a 12 anos (33%. Dentre os acidentes de trânsito a colisão (31,1% e o atropelamento (18,2% foram os mais freqüentes. Já no ambiente domiciliar os atendimentos foram devidos a destelhamentos, queda de tanque de lavar roupas, queimaduras, afogamentos, intoxicações e eventos envolvendo cavalo e abelha. Houve ainda registro devido a agressão, estupro e ferimento por arma de fogo. Conclui-se que, as crianças têm sofrido injúrias diversas decorrentes dos acidentes de trânsito, doméstico além de violências físicas e sexuais, indicando a necessidade de intensificar as estratégias voltadas à prevenção de acidentes de trânsito.

  18. Movimentos pendulares das cidades interioranas de porte médio de Cascavel e Toledo, no Paraná Movimientos pendulares de las ciudades del interior de porte medio de Cascavel y Toledo, en Paraná Commuting in medium-size cities of Cascavel and Toledo, Paraná

    Directory of Open Access Journals (Sweden)

    Cristiano Stamm

    2008-06-01

    Full Text Available O objetivo desse artigo é examinar o fenômeno do movimento pendular de trabalhadores intermunicipais entre as cidades interioranas de porte médio de Cascavel e Toledo, localizadas na Mesorregião Oeste do Estado do Paraná. A dinâmica econômico-populacional das distintas regiões do interior do Brasil vem imprimindo novas modalidades de deslocamentos populacionais, as quais eram quase exclusivas dos grandes centros urbanos. Os estudos dessa natureza tornam-se importantes, pois levantam informações relevantes para o planejamento urbano-regional e no ordenamento da dinâmica territorial. O movimento pendular observado nesse estudo é do tipo cruzado, o que significa que há movimento casa-trabalho originado nas duas cidades, em razão de ambas serem pólos regionais. O resultado da pesquisa evidenciou que a rede primária de proteção social (a família é um fator importante e, em particular, das cidades de porte médio, como é caso de Cascavel e Toledo. O fluxo de trabalhadores pendulares entre Cascavel e Toledo apresenta um papel fundamental no desenho da rede urbana da região. Desta forma, verificou-se que algumas questões urbanas apresentadas nos grandes centros urbanos e nas regiões metropolitanas do país, tais como os movimentos pendulares, são também temas importantes para as cidades de menor porte populacional.El objetivo de este artículo es examinar el fenómeno del movimiento pendular de trabajadores intermunicipales entre las ciudades del interior de porte medio de Cascavel y Toledo, localizadas en la Mesoregión Oeste del Estado de Paraná. La dinámica económico-poblacional de las distintas regiones del interior de Brasil viene imprimiendo nuevas modalidades de desplazamientos poblacionales, las cuales eran casi exclusivas de los grandes centros urbanos. Los estudios de esta naturaleza se tornan importantes, pues exponen informaciones relevantes para la planificación urbano-regional y en el ordenamiento de la din

  19. Evidência sorológica da infecção aguda pelo Toxoplasma gondii em gestantes de Cascavel, Paraná Serological evidence of acute Toxoplasma gondii infection in pregnant women in Cascavel, Paraná

    Directory of Open Access Journals (Sweden)

    Sônia de Lucena Mioranza

    2008-12-01

    Full Text Available Para verificar a ocorrência da toxoplasmose em Cascavel, Paraná, cidade próxima a região onde ocorreu o maior surto epidêmico descrito mundialmente, 334 amostras de soros de gestantes foram triadas pelo ensaio imunoenzimático comercial IgG no Laboratório Municipal de Cascavel, e confirmadas no Instituto de Medicina Tropical de São Paulo por imunofluorescência IgG, ensaio imunoenzimático e avidez de IgG in house. A soropositividade pelo IgG comercial foi 54,2%, pela imunofluorescência 54,8% e pelo IgG in house 53,9%, com boa concordância entre imunofluorescência/IgG comercial (Kappa=0,963781; co-positividade=97,8%; co-negatividade=98,7% e imunofluorescência/IgG in house (Kappa=0,975857; co-positividade=97,8%; co-negatividade=100%. A evidência de infecção aguda nas gestantes foi similar tanto pela avidez de IgG (2,4% ao ano como pela análise estatística de tendência (teste χ2 por faixa etária (2% ao ano, sugerindo que a triagem sorológica pré-natal e a vigilância epidemiológica são imprescindíveis para redução do risco da toxoplasmose na região, embora sem evidência de surto epidêmico.In order to investigate the incidence of toxoplasmosis in Cascavel, Paraná, a city near the region where the largest reported epidemic outbreak in the world occurred, 334 serum samples from pregnant women were screened using a commercial IgG immunoenzymatic assay at the Municipal Laboratory in Cascavel and were confirmed at the Institute of Tropical Medicine in São Paulo, by means of IgG immunofluorescence, immunoenzymatic assaying and the in-house IgG avidity test. The IgG seropositivity from the commercial test was 54.2%, from immunofluorescence 54.8% and from the in-house IgG 53.9%, with good agreement between immunofluorescence and the commercial IgG test (kappa = 0.963781; co-positivity = 97.8%; co-negativity = 98,7% and between immunofluorescence and the in-house IgG (kappa = 0.975857; co-positivity = 97.8%; co

  20. Avaliação qualitativa de pulverizadores da região de Cascavel, Estado do Paraná = Sprayers qualitative evaluation in chemical application in Cascavel, State of Paraná

    Directory of Open Access Journals (Sweden)

    João Cleber Modernel da Silveira

    2006-10-01

    Full Text Available No processo de tratamento fitossanitário, o controle das características da pulverização deve ser realizado com o objetivo de garantir a eficiência dos tratamentos, evitando-se perdas de produtos sem causar danos ao meio e ao próprio homem. Dessa forma, é fundamental que os pulverizadores estejam em perfeito estado de conservação. O objetivo deste trabalho foi realizar na região de Cascavel, Estado do Paraná, uma avaliação qualitativa de pulverizadores agrícolas. Foram checados, no campo, o estado e presença dos principais componentes de 62 pulverizadores. Os pulverizadores avaliados foram divididos em três categorias: montado, arrasto e autopropelido. Observou-se que os equipamentos avaliadosestão com manutenção inadequada. Considerando a presença e o estado de alguns componentes (bicos, manômetros, filtros de linhas, entre outros, apenas 17% dos equipamentos estão em condições adequadas de uso.Spray quality control should be accomplished in the process of chemical treatment, aiming to guarantee the efficiency, avoiding loss of product and damages to the environment. It is fundamental, thus, sprayers optimum conservation. This work aimed to carry out a qualitative evaluation of the sprayers in farms in the municipality of Cascavel, State of Paraná, southern Brazil. The conditions and presence (or absence of main components in 62 sprayers were checked in the field. The appraised sprayers were divided in three categories: attached to tractors, PTO-driven trailed and self-propelled. The equipments showed inadequate maintenance. Considering the presence and the conditions of some components (nozzles, filters of lines, nozzles filters, among others, only 17% of the equipment evaluated was in good condition.

  1. Therapeutic evaluation of homoeopathic drug Crotalus horridus 200C against Ehrlichiosis-infected dogs in Mizoram

    Directory of Open Access Journals (Sweden)

    Isaac B Tungnunga

    2016-01-01

    Conclusion: Nested PCR assay had been shown to be sensitive and specific for detection of Ehrlichia canis. Crotalus horridus 200C may be an effective and choice of drug for control of canine ehrlichiosis.

  2. Final Critical Habitat for the New Mexican ridge-nosed rattlesnake (Crotalus willardi obscurus)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for New Mexican ridge-nosed rattlesnake (Crotalus willardi obscurus) occur based on the...

  3. Avaliação socioeconômica e de fatores que levam a violência com detentos de Cascavel/PR Evaluación socio-económica y de factores que levan a la violencia com detenidos de Cascavel/PR Socioeconomic and factor evaluation that lead to violence with inmates of Cascavel/PR

    Directory of Open Access Journals (Sweden)

    Thiago Eugênio Armani

    2010-06-01

    Full Text Available A violência não é caracterizada apenas por sequestros, roubos, assaltos, mas também por se destruir a identidade social, cultural e econômica de um povo. A violência aprisiona a capacidade de um país evoluir, porque coloca seus jovens no mundo das drogas, da prostituição, do analfabetismo, enfim na marginalização social, pois todo o desenvolvimento de um povo só acontece graças a uma evolução social, cultural, econômica, psíquica e biológica, que é ditada principalmente pela educação empregada nas escolas. Baseado nisto este trabalho analisou as características sociais, educacionais e econômicas de sujeitos entre 18 e 60 anos que se encontram em cárcere privado na cidade de Cascavel/PR. Através da aplicação de um questionário com perguntas e respostas objetivas, que a partir do seu preenchimento forneceu ideia de quais são os possíveis fatores geradores de violência, com a pesquisa sendo realizada diretamente com os detentos. Foi constatado que há problema educacional, social e econômico com os detentos cascavelenses, porque os mesmos possuem pouca escolaridade, sofrem com desemprego, baixa renda, entre outros problemas, que influenciam a entrada para a criminalidade.La violencia no sólo se caracteriza por los secuestros, robos, asaltos, sino también por la destrucción de la identidad social, cultural y económica de las personas. La violencia aprisiona la capacidad de un país evolucionar por que coloca sus jóvenes en el mundo de las drogas, de la prostitución, del analfabetismo, en fin en la marginalización social, pues todo el desarrollo de un pueblo sólo pasa a través de la evolución social, cultural, económica, psicológica y biológica, que es dictada principalmente por la educación en las escuelas. Este trabajo analizó las características sociales, educacionales y económicas de sujetos entre 18 y 60 años que están en una casa de detención en la ciudad de Cascavel/PR. Mediante la aplicaci

  4. Formas de uso medicinal da babosa e camomila pela população urbana de Cascavel, Estado do Paraná = Medicinal use of the Aloe and Chamomile for the urban population of Cascavel, Paraná State

    Directory of Open Access Journals (Sweden)

    Claudia Tatiana Araujo da Cruz-Silva

    2010-07-01

    Full Text Available A etnobotânica se caracteriza por buscar entender a relação entre as plantas e o homem, podendo-se através desta conhecer a utilização das plantas medicinais como forma de tratamento. O objetivo deste trabalho foi realizar um levantamento sobre as formas de utilização da Babosa (Aloe vera L. e da Camomila (Matricaria chamomilla L.. A coleta de dados envolveu entrevistas de forma aleatória a 400 pessoas, através de um questionário semi-estruturado, no município de Cascavel, Estado do Paraná. Observou-se que 65% da população utilizam à babosa ou a camomila na cura ou alívio de doenças. A maioria dosentrevistados possui renda de 2 a 4 salários mínimos (60,25%, idade entre 28 a 38 anos (30,75%, tendo concluído o ensino médio (33,75%. O principal motivo pelo qual a população se utiliza de plantas medicinais é por ser natural (71,84%. A forma de preparo mais freqüente da camomila foi por infusão (63,38%, utilizando as flores (92%. Para a babosa 100% utilizam suas folhas na forma de cataplasma (43,88%. Apenas 3% da população relataram ter apresentado algum tipo de reação adversa, durante o período de utilização. Conclui-se que o uso destas plantas pela população é freqüente, sendo um recurso adicional ao uso de medicamentos.The ethnobotany, which is characterized by look for to understand the relationship between the plants and the man, being been able through this to know the use of the medicinal plants as treatment. The objective of this work was to accomplish a rising on the forms of use of the Aloe (Aloe vera L. and of the Chamomile (Matricaria chamomilla L.. The collection of data involved interviews in a random way to a sample of 400 people through a semi-structured questionnaire, in the city of Cascavel, Paraná State. It was observed that 65% of the population use to aloe or the chamomile in the cure or relief of diseases. Most of the interviewees possesses income from 2 to 4 minimum wages (60.25%, age

  5. Eficiência de um conjunto fotovoltaico em condições reais de trabalho na região de Cascavel = Efficiency of the photovoltaic systems under real work conditions on region of Cascavel

    Directory of Open Access Journals (Sweden)

    Estor Gnoatto

    2008-07-01

    Full Text Available No presente trabalho, foi desenvolvido um estudo sobre um sistemafotovoltaico autônomo, com armazenamento de energia, instalado na Estação Experimental Agrometeorológica da Universidade Estadual do Oeste do Paraná, Campus Cascavel, a uma Latitude 24º59’ Sul, Longitude de 53º26’ Oeste, com altitude de 682 m, em condições reaisde campo, durante o ano de 2002. Foi avaliada a eficiência dos painéis fotovoltaicos em condições reais de campo, sua curva característica foi determinada utilizando, na aquisição de dados, um micrologger da Campbell Scientific-Inc, modelo CR10X, programado pararealizar uma leitura por segundo de cada canal e armazenar a média aritmética de 5 minutos dos dados das componentes de radiação solar global no plano horizontal e global na incidência do painel; temperatura ambiente e temperatura de operação do painel; tensão ecorrente do sistema fotovoltaico. Os resultados mostram que a eficiência média mensal registrou valores na faixa de 8,65 a 9,17%, com uma média de 8,84%, com variação percentual entre o índice máximo e mínimo de 5,67%. Pode, assim, representar fornecimento médio mensal de energia de 5,57 kWh por painel.The purpose of the present work was to develop a study of anautonomous photovoltaic system, with energy storage, installed at the Experimental Agrometeorological Station of the Universidade Estadual do Oeste do Paraná, Cascavel Campus, located at 24º59’S 53º26’W, 682 meters of altitude, under real field conditions, during the year of 2002. The efficiency of the photovoltaic modules was evaluated underreal field conditions and its characteristic linear fit was determined. A Campbell Scientific CR10X micrologger was used to acquire data, programmed to conduct one reading per second from each channel and store the five-minute arithmetic data average from thereadings of the incidence of global direct solar radiation horizontal and global on the panel, as well as area

  6. A "Pedagogia" da Feira Livre de SÃo Bento: narrativas, saberes e prÃticas educativas na cidade de Cascavel - CE

    OpenAIRE

    Francisca Eliana Santos da Silva

    2014-01-01

    Neste estudo, buscamos compreender as prÃticas educativas e os saberes presentes na feira livre de SÃo Bento, localizada na cidade de Cascavel-CE a qual dista de Fortaleza 64 km. Para a realizaÃÃo dessa pesquisa, escolhemos como suporte teÃrico BrandÃo (2005); Durkheim (2007); Burke (2003); Huberman (1976) e Certeau (1998). Optamos pelo enfoque qualitativo e utilizamos a pesquisa bibliogrÃfica como suporte no esclarecimento do tema investigado. Usamos tambÃm o estudo de caso que proporcionou ...

  7. Occurrence of Cryptosporidium spp. in wild animals living in the Cascavel city park, Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Alessandra Snak

    2015-12-01

    Full Text Available Digestive and respiratory tracts parasite’s protozoan, Cryptosporidium spp. now-a-days is a major zoonotic agent, it causes self-limiting diarrhea, remaining in the body passively until the moment immune system decreases, leading to an increase in its multiplication in the mucosa and appearance of clinical signs. As there are few studies on cryptosporidiosis in wild free-living and captive animals, especially in Brazil, this study aimed to identify the presence of Cryptosporidium spp. in feces of captive animals in Cascavel, PR Municipal Zoo. Between 2011 and 2012 there have been four collections of bird feces and five mammalian feces totaling 65 and 118 samples respectively. Samples were sent to the laboratory of Veterinary Parasitology of Federal University of Parana. The feces were diluted, centrifuged and the pellets were used to make blades which were stained by the Ziehl-Neelsen modified method and observed under a microscope with 1000X magnification. Then the blades containing the oocysts were observed under a capture microscope, where they were measured. Mammals showed 49.15% and birds 44.61% of positivity. Oocysts’ sizes ranged from 3,54?m to 5,81?m with an average of 4,32?m for birds and 3,11?m to 5,60?m averaging 4,63?m to mammals. As of yet, there isn’t effective treatment against this parasite and considering that it’s a zoonotic disease, preventive measures should be taken to prevent transmission to humans.

  8. Chemosensory response in stunted prairie rattlesnakes Crotalus viridis viridis

    Institute of Scientific and Technical Information of China (English)

    Anthony J.SAVIOLA; David CHISZAR; Hobart M.SMITH; Stephen P.MACKESSY

    2013-01-01

    Rattlesnakes use chemical stimuli in ambush site selection and for relocation of envenomated prey through strike-induced chemosensory searching.Shifts in responsiveness to prey chemicals have been documented in many snakes,and often correlate with prey commonly taken as snakes increase in age and size as well as geographical locations of the species.For instance,neonate rattlesnakes that prey primarily on ectotherms responded most strongly to chemical cues of commonly taken lizard prey,whereas adult rattlesnakes that prey primarily on small mammals responded significantly to chemical cues of commonly taken rodents.In the current study,11 Prairie Rattlesnakes Crotalus viridis viridis which were classified as large neonates based on measures of snout-vent length (SVL) and body mass,yet chronologically were at or near adulthood,were tested for their responsiveness to chemical extracts of natural and non-natural prey items.Although the snakes had eaten only neonate lab mice (Mus musculus),they responded significantly more to chemical cues of natural prey items and particularly to chemical cues of prey normally taken by subadults (Peromyscus mice and Sceloporus lizard).These results suggest that ontogenetic shifts in responsiveness to natural prey chemical cues are innately programmed and are not based on body size or feeding experience in C.v.viridis.This does not imply,however,that growth and experience are without effects,especially with novel prey or rare prey that have experienced recent population expansion.

  9. Crotalus durissus terrificus venom as a source of antitumoral agents

    Directory of Open Access Journals (Sweden)

    MA Soares

    2010-01-01

    Full Text Available The basic knowledge on neoplasms is increasing quickly; however, few advances have been achieved in clinical therapy against tumors. For this reason, the development of alternative drugs is relevant in the attempt to improve prognosis and to increase patients' survival. Snake venoms are natural sources of bioactive substances with therapeutic potential. The objective of this work was to identify and characterize the antitumoral effect of Crotalus durissus terrificus venom (CV and its polypeptide, crotoxin, on benign and malignant tumors, respectively, pituitary adenoma and glioblastoma. The results demonstrated that CV possess a powerful antitumoral effect on benign (pituitary adenoma and malignant (glioblastoma multiforme tumors with IC50 values of 0.96 ± 0.11 µg/mL and 2.15 ± 0.2 µg/mL, respectively. This antitumoral effect is cell-cycle-specific and dependent on extracellular calcium, an important factor for crotoxin phospholipase A2 activity. The CV antitumoral effect can be ascribed, at least partially, to the polypeptide crotoxin that also induced brain tumor cell death. In spite of the known CV nephrotoxicity and neurotoxicity, acute treatment with its antitumoral dose established in vitro was not found to be toxic to the analyzed animals. These results indicate the biotechnological potential of CV as a source of pharmaceutical templates for cancer therapy.

  10. DSC analysis of irradiated proteins from Crotalus durissus terrificus

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto de; Silva, Monica Nascimento da; Goncalves, Karina de Oliveira; Spencer, Patrick Jack; Nascimento, Nanci do [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    Full text: Snake bites are a serious public health problem, especially in subtropical countries. In Brazil, the serum, the only effective treatment in case of snake bites, is produced in horses which, despite the large size, have a reduced lifespan due to the high toxicity of the antigen. It is known that ionizing radiation effects - direct and indirect - can modify the molecular structure, affecting the biological properties of proteins. Ionizing radiation has been employed to attenuate the toxicity of snake venoms, aiming to generate an improved antigen with low toxicity. Two proteins, purified from Crotalus durissus terrificus (Cdt) venom were tested in this work: crotoxin and crotamine. Crotoxin, the main toxic compound of Cdt venom, is a heterodimeric protein composed of two subunits: crotapotin and phospholipase A2. Crotamine is a highly basic polypeptide (pI - 10.3), with myotoxic activity and molecular weight of 4882 Da. It is composed of 42 amino acids residues and reticulated by three disulfide bonds. This study aimed to investigate the effects of radiation on crotoxin and crotamine using Differential Scanning Calorimetry (DSC). After isolation of the toxins by chromatographic techniques, they were irradiated with 2.0 kGy from {sup 60}Co source. The thermodynamics analysis, carried out in a METTLER TOLEDO, DSC 822e calorimeter, showed that irradiation promoted changes of the calorimetric profile. These changes suggest that, although radiation induced structural modifications of the protein, denaturation was only partial, since transition states could still be detected, suggesting that some structural elements were still present after irradiation. Taken together, our data suggest that following irradiation, the molecules underwent conformational changes, and that the remaining structural elements displayed a lower enthalpy, clearly indicating that the previously described loss of toxicity of irradiated toxins can be mostly ascribed to structural changes

  11. Snake venomics of the Central American rattlesnake Crotalus simus and the South American Crotalus durissus complex points to neurotoxicity as an adaptive paedomorphic trend along Crotalus dispersal in South America.

    Science.gov (United States)

    Calvete, Juan J; Sanz, Libia; Cid, Pedro; de la Torre, Pilar; Flores-Díaz, Marietta; Dos Santos, M Cristina; Borges, Adolfo; Bremo, Adolfo; Angulo, Yamileth; Lomonte, Bruno; Alape-Girón, Alberto; Gutiérrez, José María

    2010-01-01

    We report a comparative venomic and antivenomic characterization of the venoms of newborn and adult specimens of the Central American rattlesnake, Crotalus simus, and of the subspecies cumanensis, durissus, ruruima, and terrificus of South American Crotalus durissus. Neonate and adult C. simus share about 50% of their venom proteome. The venom proteome of 6-week-old C. simus is predominantly made of the neurotoxic heterodimeric phospholipase A(2) (PLA(2) crotoxin) (55.9%) and serine proteinases (36%), whereas snake venom Zn(2+)-metalloproteinases (SVMPs), exclusively of class PIII, represent only 2% of the total venom proteins. In marked contrast, venom from adult C. simus comprises toxins from 7 protein families. A large proportion (71.7%) of these toxins are SVMPs, two-thirds of which belong to the PIII class. These toxin profiles correlate well with the overall biochemical and pharmacological features of venoms from adult (hemorrhagic) and newborn (neurotoxic) C. simus specimens. The venoms of the South American Crotalus subspecies belong to one of two distinct phenotypes. C. d. cumanensis exhibits high levels of SVMPs and low lethal potency (LD(50)), whereas C. d. subspecies terrificus, ruruima, and durissus have low SVMP activity and high neurotoxicity to mice. Their overall toxin compositions explain the outcome of envenomation by these species. Further, in all C. simus and C. durissus venoms, the concentration of neurotoxins (crotoxin and crotamine) is directly related with lethal activity, whereas lethality and metalloproteinase activity show an inverse relationship. The similar venom toxin profiles of newborn C. simus and adult C. durissus terrificus, ruruima, and durissus subspecies strongly suggests that the South American taxa have retained juvenile venom characteristics in the adult form (paedomorphism) along their North-South stepping-stone dispersal. The driving force behind paedomorphism is often competition or predation pressure. The increased

  12. Body size evolution in insular speckled rattlesnakes (Viperidae: Crotalus mitchellii.

    Directory of Open Access Journals (Sweden)

    Jesse M Meik

    Full Text Available BACKGROUND: Speckled rattlesnakes (Crotalus mitchellii inhabit multiple islands off the coast of Baja California, Mexico. Two of the 14 known insular populations have been recognized as subspecies based primarily on body size divergence from putative mainland ancestral populations; however, a survey of body size variation from other islands occupied by these snakes has not been previously reported. We examined body size variation between island and mainland speckled rattlesnakes, and the relationship between body size and various island physical variables among 12 island populations. We also examined relative head size among giant, dwarfed, and mainland speckled rattlesnakes to determine whether allometric differences conformed to predictions of gape size (and indirectly body size evolving in response to shifts in prey size. METHODOLOGY/PRINCIPAL FINDINGS: Insular speckled rattlesnakes show considerable variation in body size when compared to mainland source subspecies. In addition to previously known instances of gigantism on Angel de la Guarda and dwarfism on El Muerto, various degrees of body size decrease have occurred frequently in this taxon, with dwarfed rattlesnakes occurring mostly on small, recently isolated, land-bridge islands. Regression models using the Akaike information criterion (AIC showed that mean SVL of insular populations was most strongly correlated with island area, suggesting the influence of selection for different body size optima for islands of different size. Allometric differences in head size of giant and dwarf rattlesnakes revealed patterns consistent with shifts to larger and smaller prey, respectively. CONCLUSIONS/SIGNIFICANCE: Our data provide the first example of a clear relationship between body size and island area in a squamate reptile species; among vertebrates this pattern has been previously documented in few insular mammals. This finding suggests that selection for body size is influenced by changes in

  13. Avaliação dos níveis de iluminação natural e artificial nas residências convencional e inovadora do ‘Projeto CASA’, Unioeste, campus de Cascavel, Estado do Paraná = Evaluation of natural and artificial lighting in conventional and innovative residences of ‘Projeto CASA’, Unioeste, Cascavel Campus, Paraná

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Camargo Nogueira

    2010-07-01

    Full Text Available Este trabalho teve por objetivo comparar os níveis de iluminação natural e artificial nas residências convencional e inovadora do ‘Projeto CASA’ (Centro de Análise de Sistemas Alternativos de Energia da Unioeste – campus de Cascavel. As medidas foram realizadas com a utilização de um Luxímetro digital, nos diversos cômodos das residências, e em diferentes horas do dia. Como resultado, verificou-se que a residência inovadora apresentou maior uniformidade na distribuição da iluminância, estando seus níveisadequados aos valores mínimos estabelecidos na NBR 5413.The purpose of this work was to compare the natural and artificial lighting levels in the conventional and innovative residences of ‘Projeto CASA’ (Center for the Analysis of Alternative Energy Systems of Unioeste – Cascavel campus. The measures were carriedout using a digital light meter, in the several rooms of the residences, at different hours of the day. It was verified that the innovative residence presented a larger uniformity in lighting distribution, being in agreement with the minimum values established in NBR 5413.

  14. Estudo clínico e laboratorial de pacientes com artrite reumatoide diagnosticados em serviços de reumatologia em Cascavel, PR, Brasil Clinical and laboratory features of patients with rheumatoid arthritis diagnosed at rheumatology services in the Brazilian municipality of Cascavel, PR, Brazil

    Directory of Open Access Journals (Sweden)

    Juliano Maximiano David

    2013-02-01

    Full Text Available INTRODUÇÃO: Estudos epidemiológicos brasileiros sobre artrite reumatoide são bastante escassos, e os dados existentes hoje são majoritariamente de literatura internacional. OBJETIVOS: Determinar a incidência e algumas características clínicas e laboratoriais de pacientes com artrite reumatoide em Cascavel, PR, Brasil. PACIENTES E MÉTODOS: Os dados foram coletados entre agosto de 2010 e julho de 2011 em todos os serviços de saúde do município que possuíam atendimento na especialidade de reumatologia: um hospital universitário, o Centro Regional de Especialidades do Consórcio Intermunicipal de Saúde do Oeste do Paraná (CRE-CISOP e quatro clínicas privadas da cidade. RESULTADOS: Foram identificados 38 pacientes com diagnóstico de artrite reumatoide, resultando em uma incidência estimada de 13,4 casos/100.000 habitantes/ano. Trinta e dois pacientes eram do gênero feminino, com média de idade de 47,6 anos. A faixa etária com maior incidência foi > 40 anos. O tempo médio entre os primeiros sintomas e o diagnóstico foi de 12,4 meses. O fator reumatoide foi positivo em 68,4% dos casos, e 18,4% já apresentavam alterações radiológicas no momento do diagnóstico. O tratamento farmacológico dos pacientes também foi avaliado e mostrou estar de acordo com o encontrado na literatura. CONCLUSÃO: A incidência de artrite reumatoide obtida em Cascavel está abaixo das incidências observadas em estudos internacionais.INTRODUCTION: Brazilian epidemiological studies on rheumatoid arthritis are scarce, thus all data currently available originate from the international literature. OBJECTIVES: To determine the incidence and some clinical and laboratory characteristics of patients with rheumatoid arthritis in the municipality of Cascavel, state of Paraná, Brazil. PATIENTS AND METHODS: Data were collected between August 2010 and July 2011 in all health services of the municipality of Cascavel that provided health care in Rheumatology: a

  15. Histopathological evaluation in experimental envenomation of dogs with Crotalus durissus terrificus venom

    Directory of Open Access Journals (Sweden)

    F. Sangiorgio

    2008-01-01

    Full Text Available The present work evaluated histopathological aspects in experimental envenomation of dogs with Crotalus durissus terrificus venom. Twenty-eight mixed breed adult dogs were divided into three groups of seven animals each: Group I - only venom; Group II - venom + 50ml antiophidic serum + fluid therapy; Group III - venom + 50ml antiophidic serum + fluid therapy + urine alkalization. Lyophilized venom of Crotalus durissus terrificus was reconstituted in saline solution and inoculated subcutaneously at the dose of 1mg/kg body weight. Three animals of each group were subjected to euthanasia, and their muscular tissue, brain, spleen, kidneys, heart, lungs, stomach, small and large intestines, and popliteal lymph node fragments were collected for histopathological evaluation. There was myonecrosis in the inoculated limb, renal tubular degeneration, lymphoid hyperplasia of spleen, and unspecific reactive hepatitis. These results show the antigenicity and action of the venom on the immune system.

  16. Crotalus aquilus in the Mexican state of Mexico consumes a diverse summer diet

    Science.gov (United States)

    Mociño-deloya, E.; Setser, K.; Peurach, S.C.; Meik, J.M.

    2008-01-01

    We report observations of the summer diet of Crotalus aquilus (Queretaro dusky rattlesnake) from an agricultural region near San Pedro de los Metates, municipality of Acambay, state of Mexico, Mexico. We recovered the remains of 12 individual prey items from 11 different snakes. Eleven of 38 (29%) snakes observed contained prey remains, including 6 mammals, 3 lizards, and 3 snakes. These observations suggest that C. aquilus consumes a diverse diet and that they may be more ophiophagous than many other rattlesnakes.

  17. The Evolutionary Implications of Hemipenial Morphology of Rattlesnake Crotalus durissus terrificus (Laurent, 1768 (Serpentes: Viperidae: Crotalinae.

    Directory of Open Access Journals (Sweden)

    Marcovan Porto

    Full Text Available Most amniotes vertebrates have an intromittent organ to deliver semen. The reptile Sphenodon and most birds lost the ancestral penis and developed a cloaca-cloaca mating. Known as hemipenises, the copulatory organ of Squamata shows unique features between the amniotes intromittent organ. They are the only paired intromittent organs across amniotes and are fully inverted and encapsulated in the tail when not in use. The histology and ultrastructure of the hemipenes of Crotalus durissus rattlesnake is described as the evolutionary implications of the main features discussed. The organization of hemipenis of Crotalus durissus terrificus in two concentric corpora cavernosa is similar to other Squamata but differ markedly from the organization of the penis found in crocodilians, testudinata, birds and mammals. Based on the available data, the penis of the ancestral amniotes was made of connective tissue and the incorporation of smooth muscle in the framework of the sinusoids occurred independently in mammals and Crotalus durissus. The propulsor action of the muscle retractor penis basalis was confirmed and therefore the named should be changed to musculus hemipenis propulsor.The retractor penis magnus found in Squamata has no homology to the retractor penis of mammals, although both are responsible for the retraction of the copulatory organ.

  18. Functional and structural analysis of two fibrinogen-activating enzymes isolated from the venoms of Crotalus durissus terrificus and Crotalus durissus collilineatus

    Institute of Scientific and Technical Information of China (English)

    Daniela G.L.de Oliveira; Mário T.Murakami; Adelia C.O.Cintra; Jo(a)o J.Franco; Suely V.Sampaio; Raghuvir K.Arni

    2009-01-01

    Fibrinogen-activating enzymes,widely distributed in Crotalidae and Viperidae venoms,are single-chain glycosylated serine proteases that display high macromolecular selectivity and are often referred to as thrombin-like enzymes(TLEs).TLEs serve as structural models to extend our understanding of the structure-function relationships of blood coagulation factors,have been clinically used for the treatment of thrombotic diseases,and are used as tools in clinical assays.The combination of gel filtration and ion-exchange chromatography proved to be successful in obtaining milligram quantities of pure samples of TLEs from the venoms of Crotalus durissus terrificus(white venom)and Crotalus durissus collilineatus(yellow venom).Functional characterization indicates that both enzymes preferentially degrade the Bβ chain of bovine fibrinogen and possess edema-inducing and coagulant activities.However,the TLE from C.d.collilineatus venom shows twofold higher coagulant activity with a minimum coagulant dose(MCD)of 0.6 μg/μl,whereas the enzyme isolated from C.d terrificus indicated an MCD of 1.5μg/μl.Molecular modeling of gyroxin and structural comparisons with other highly conserved snake venom serine proteases,underlines the key role played by the surface charge distribution and the double insertion in the 174-surface loop in macromolecular substrate recognition by TLEs.

  19. Avaliação farmacognóstica e microbiológica da droga vegetal camomila (Chamomilla recutita L. comercializada como alimento em Cascavel - Paraná Pharmacognostic and microbiological evaluation of the medicinal plant chamomile (Chamomilla recutita L. commercialized as food in Cascavel Municipality, Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    P.S.R Lucca

    2010-06-01

    Full Text Available As plantas medicinais, dentre elas a camomila, têm sido muito utilizadas na forma de droga vegetal pela população em geral, como importantes alternativas alimentícias e terapêuticas. Considerando que a fiscalização sanitária destes produtos é precária, este quadro torna-se preocupante, visto que, um produto em condições inadequadas para consumo pode acarretar vários riscos ao consumidor. Diante disso, este trabalho teve por objetivo realizar avaliação farmacognóstica e microbiológica em quinze amostras de chá de camomila comercializadas na cidade de Cascavel, Paraná. A metodologia adotada para a realização dos testes foi a preconizada pela Farmacopéia Brasileira (1988, 1996, 1998. O estudo de autenticidade revelou que todas as amostras eram constituídas por Chamomilla recutita L., porém na maioria das amostras os capítulos florais apresentavam-se excessivamente destruídos. Seis amostras apresentaram teor de materiais estranhos acima de 5%. Quanto ao doseamento de óleo essencial, todas as amostras foram insatisfatórias, visto que, apresentaram apenas traços de óleos essenciais, ou seja, valores abaixo de 0,4%, sendo este valor o mínimo exigido pela Farmacopéia. Com relação às análises de bolores e leveduras foi verificado que quatro amostras apresentaram valores acima do limite de 10(4 UFC mL-1, conforme preconizado pela Organização Mundial de Saúde. A partir dos dados obtidos concluiu-se que a camomila comercializada na cidade de Cascavel - Paraná apresenta problemas com relação à qualidade, sendo necessário definir medidas adequadas de controle higiênico sanitário ao longo da cadeia de produção, para garantir a qualidade e segurança destes produtos.Medicinal plants, including chamomile, have been largely used by the general population as important food and therapeutic alternatives. Considering that the sanitary control of such products is precarious, this situation becomes worrisome since a

  20. South American energy integration: new perspectives; Novas perspectivas para a integracao energetica sul-americana

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Jose Malhaes da [Malhaes da Silva Consultoria Ltda., Rio de Janeiro, RJ (Brazil); Salomao, Luiz Alfredo [Universidade Candido Mendes, Rio de Janeiro, RJ (Brazil). Escola de Politicas Publicas e Governo

    2008-07-01

    This paper analyses the South American energy integration, the advantages related to this process as well as the existing obstacles . What is the scope in terms of projects and initiatives, and the role of political and institutional barriers to be overcome. (author)

  1. AVALIAÇÃO NUTRICIONAL DE PRATICANTES DE MUSCULAÇÃO COM OBJETIVO DE HIPERTROFIA MUSCULAR DO MUNICÍPIO DE CASCAVEL, PR

    Directory of Open Access Journals (Sweden)

    Ana Flávia de Oliveira1

    2009-06-01

    Full Text Available This study aimed to assess diet and investigate the use of nutritional supplements and anabolic steroids in practicing workout. Eleven male gender individuals were evaluated for who practice workout with the objective of muscle hypertrophy of the municipality of Cascavel – Paraná. The dietary evaluation was carried out through dietary investigators. The micronutrients intake, macronutrients and dietary fibers were analyzed according to the Recommended Dietary Allowances. It was collected information about the use of nutritional supplements and anabolic steroids. In regard to the nutritional orientation only four (36.4% individuals have received this type of service. Regarding the use of food supplements, five (45.5% make use, and cited seven different types. Regarding the use of anabolic steroids three of the individuals (27.3% reported using or have already made use Eight (72.7% had energy consumption more than 3000 kcal/d and the average of micronutrients intake presented a non balanced diet, low in carbohydrates (40.2 ± 8.4% and high in fat (36.0 ± 5.9%. Through this study, it was observed that the individuals evaluated are conscious about the appropriate food to obtain muscle hypertrophy, there is erroneous eating habits, indicating possible risks to health in the long term and even decrease in athletic performance.

  2. Os jornais diários da cidade de Cascavel (PR e seus discursos sobre a prostituição feminina (1976-1990

    Directory of Open Access Journals (Sweden)

    Fábio Lopes Alves

    2010-01-01

    Full Text Available The object of the present paper is to investigate the representations of female prostitution in the periodical press of Cascavel (PR, Brazil. The journals analyzed are the newspapers Hoje and O Paraná. It aims to understand how some women, who do not figure in the annals of the feminist movement, that don't have an exceptional and exemplary trajectory to the young ladies and not even show up on the social columns of the press, are represented by these media. In this context, this discussion is structured as follows, at first, it establishes a dialog with Roger Chartier to reach the representation concept. After that, the subject will be about how the female prostitution was represented by written press. At this point, the article highlights the journalistic reports that alluded to the risk of social contagion offered by these women to the society, while they are catching the AIDS and syphilis virus. At the end, it interacts with the philosopher Michel Foucault, to realize how the disciplining of the bodies through journalistic speeches happened.

  3. Purification and radiodination of gyroxin, toxin like trombin, of crotalus durissus terrificus venom; Purificacao e radioiodacao da giroxina (toxina semelhante a trombina) do veneno de crotalus durissus terrificus

    Energy Technology Data Exchange (ETDEWEB)

    Camillo, Maria A.P.; Paes, Paulo C.A.; Ribela, Maria T.C.P.; Rogero, Jose R. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    1997-12-01

    Snake`s venoms have attracted special interest in research because they are rich in bioactive compounds, which are scientific tools to study many physiological processes and are also important source of new therapeutic agents. Some thrombin-like enzymes are used as a reagent in laboratorial assays as well as in medical drugs. However, a neurotoxic syndrome called gyroxin syndrome or barrel rotation seems to be related to those enzymes. Pharmacokinetics and biodistribution studies of these toxins can contribute to clarifly this mechanisms and consequently, it will help to elaborate safer clinical prescriptions giving more information about possible nocive effects. This paper describes the first step of these studies with the thrombin-like enzyme (or gyroxin) from Crotalus durissus terrificus`s venom. The purification and radioiodination methods as well as electrophoretic analysis and biological assay are presented. (author). 9 refs., 5 figs., 1 tab.

  4. Vascular effects and electrolyte homeostasis of the natriuretic peptide isolated from Crotalus oreganus abyssus (North American Grand Canyon rattlesnake) venom

    NARCIS (Netherlands)

    Da Silva, S.L.; Dias-Junior, C.A.; Baldasso, P.A.; Damico, D.C.; Carvalho, B.M.; Garanto, A.; Acosta, G.; Oliveira, E.; Albericio, F.; Soares, A.M.; Marangoni, S.; Resende, R.R.

    2012-01-01

    Crotalus oreganus abyssus is a rattlesnake that is usually found in the Grand Canyon, United States of America. Knowledge regarding the composition of C. o. abyssus venom is scarce. New natriuretic peptides (NPs) have been isolated and characterized from the venoms of members of the Crotalinae famil

  5. Qualidade de medicamentos isentos de prescrição: um estudo com marcas de dipirona comercializadas em uma drogaria de Cascavel (PR, Brasil Quality of over-the-counter medicines: a study with dipyrone brands commercialized in a drugstore in Cascavel city (Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    André Leandro Knappmann

    2010-11-01

    Full Text Available A dipirona é um fármaco muito utilizado pela população brasileira. É considerado seguro mesmo em gestantes, lactentes e crianças, mas é proibido em alguns países do mundo pelo suposto papel de causar agranulocitose e anemia aplástica. Em 2001, a Agência Nacional de Vigilância Sanitária (Anvisa considerou que os medicamentos com esse fármaco apresentavam bom risco-benefício em relação a outros de indicação semelhante. Porém, de nada adianta segurança no uso de um medicamento se este é de baixa qualidade. É comum encontrar no mercado brasileiro medicamentos fora dos padrões, o que se constitui em um risco para a população em geral. Dessa forma, foram analisadas sete amostras de marcas diferentes de dipirona solução oral comercializados em uma farmácia de Cascavel (PR. Os resultados demonstram que a fiscalização quanto à qualidade de medicamentos similares precisa ser aprimorada, pois eles foram os que mais apresentaram desvios de qualidade.Dipyrone is an antipyretic and analgesic medicine very used by the Brazilian population. The administration is considered safe even in pregnant women, nurseling and children, but is forbidden in some countries, as supposedly causes agranulocytosis and aplastic anemia. In 2001, National Health Surveillance Agency (Anvisa approved the commercialization of this medicine in Brazil. However, it does not matter the safeness in the use of a medicine advances, if it does not have quality. Based on this quality, this work was elaborated, that analyzes seven samples of commercialized different marks of dipyrone oral solution in pharmaceutical establishment in the Cascavel city, Paraná, Brazil. The results demonstrate that the quality control of similar drugs must be improved as were the ones that presented quality deviations.

  6. Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study

    Directory of Open Access Journals (Sweden)

    Priscilla Brunelli Pujatti

    2005-10-01

    Full Text Available Technetium-99m (99mTc has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, 99mTc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with 99mTc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological activity was preserved. The results suggest that 99mTc-CV can be a useful tool for biodistribution studies.Tecnécio-99m tem sido o radioisótopo de escolha para procedimentos médicos e pesquisas experimentais. Em decorrência de suas propriedades nucleares, 99mTc é adequado para detecção de alta eficiência com a vantagem do baixo risco radiológico. O veneno de Crotalus (CV apresentou propriedades antitumorais em estudos clínicos e estudos de biodistribuição são fundamentais em pesquisas clínicas. Esse trabalho teve como objetivo obter um análogo de veneno de Crotalus marcado com 99mTc que preservasse sua atividade biológica. Após a marcação, a atividade biológica foi avaliada através do ensaio de atividade hemolítica. Veneno nativo e marcado apresentaram atividade hemolítica indireta quando incubados em um meio contendo uma fonte exógena de lecitina. Obteve-se um alto rendimento de marcação e a atividade biológica das moléculas foi preservada. Nossos resultados sugerem que 99mTc-CV pode representar uma ferramenta muito útil para estudos de biodistribuição.

  7. Daily and seasonal activity patterns of free range South-American rattlesnake (Crotalus durissus

    Directory of Open Access Journals (Sweden)

    ALEXANDRO M. TOZETTI

    2013-09-01

    Full Text Available This study aimed at describing daily and seasonal variation in the activity of a population of South-American rattlesnakes (Crotalus durissus in a savanna like habitat (Cerrado in Southeastern Brazil. Seasonal and daily activities of snakes were evaluated by the number of captures of snakes during road surveys, accidental encounters, and relocations by radio-tracking. Our results show that climatic variables such as air temperature and rainfall have little influence on the activity pattern of rattlesnakes. Our findings indicate that rattlesnakes spend most of the day resting and most of the night in ambush posture. The South-American rattlesnake is active throughout the year with a discrete peak in activity of males during the matting season. The possibility of maintaining activity levels even during the coldest and driest season can facilitate the colonization of several habitats in South America. This possibility currently facilitates the colonization of deforested areas by rattlesnakes.

  8. A taxonomic bibliography of the South American snakes of the Crotalus durissus complex (Serpentes, Viperidae

    Directory of Open Access Journals (Sweden)

    VANZOLINI PAULO E.

    2002-01-01

    Full Text Available A survey is made of the taxonomic literature on South American rattlesnakes (genus Crotalus, family Viperidae. Two main areas are emphasized: the attribution of the names proposed in the eighteenth century by Linnaeus and Laurenti, and the current scheme of division in subspecies. The attribution of names is examined based on the original descriptions and on relevant previous and contemporary literature. The presently adopted scheme, proposed by Klauber (1941, 1972 is found not entirely satisfactory, but reasonable enough - besides being hallowed by use. The scheme of geographical differentiation, intrinsically important and with broad practical implications (differentiation of the venom is found to be the culmination of a long series of deficient analyses, and in urgent need of proper investigation.

  9. Kinetic characterization of gyroxin, a serine protease from Crotalus durissus terrificus venom.

    Science.gov (United States)

    Yonamine, Camila M; Kondo, Marcia Y; Juliano, Maria A; Icimoto, Marcelo Y; Baptista, Gandhi R; Yamane, Tetsuo; Oliveira, Vitor; Juliano, Luis; Lapa, Antônio J; Lima-Landman, Maria Teresa R; Hayashi, Mirian A F

    2012-12-01

    This work describes for the first time the characterization of the enzymatic features of gyroxin, a serine protease from Crotalus durissus terrificus venom, capable to induce barrel rotation syndrome in rodents. Measuring the hydrolysis of the substrate ZFR-MCA, the optimal pH for proteolytic cleavage of gyroxin was found to be at pH 8.4. Increases in the hydrolytic activity were observed at temperatures from 25 °C to 45 °C, and increases of NaCl concentration up to 1 M led to activity decreases. The preference of gyroxin for Arg residues at the substrate P1 position was also demonstrated. Taken together, this work describes the characterization of substrate specificity of gyroxin, as well as the effects of salt and pH on its enzymatic activity.

  10. Ecology and behavior of the Midget Faded Rattlesnake (Crotalus oreganus concolor) in Wyoming

    Science.gov (United States)

    Parker, J.M.; Anderson, S.H.

    2007-01-01

    We conducted a three-year study to describe the ecology and behavior of the Midget Faded Rattlesnake, Crotalus organus concolor. We encountered 426 and telemetered 50 C. o. concolor between 2000 and 2002. We found that their primary diet was lizards (associated with rock outcrops), though they will consume small mammals and birds. They den in aggregations, although in low numbers when compared to other subspecies. Movements and activity ranges were among the largest reported for rattlesnakes. Minimum convex polygon area was 117.8 ha for males, 63.9 ha for nongravid females, and 4.8 ha for gravid females. Mean distances traveled per year were 2122.0 m for males, 1956.0 m for nongravid females, and 296.7 m for gravid and postpartum females. Following emergence from hibernation, they spent several weeks shedding, often in aggregations before migration, and migrations occurred in early summer. Most snakes made straight-line movements to and from discrete summer activity ranges where short, multidirectional movements ensued, although others made multidirectional movements throughout the active season. We observed mating behavior between 21 July and 12 August. Gravid females gave birth during the third week of August. Mean clutch size was 4.17 (range 2-7). We found that the sex ratio was skewed favoring females 1:1.24, and they were sexually dimorphic in size (males SVL = 44.1 cm; females SVL = 40.8 cm). Our data further illustrate the diversity within the large group of Western Rattlesnakes (Crotalus viridis). Copyright 2007 Society for the Study of Amphibians and Reptiles.

  11. Intrahippocampal Infusion of Crotamine Isolated from Crotalus durissus terrificus Alters Plasma and Brain Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    Rithiele Gonçalves

    2014-11-01

    Full Text Available Crotamine is one of the main constituents of the venom of the South American rattlesnake Crotalus durissus terrificus. Here we sought to investigate the inflammatory and toxicological effects induced by the intrahippocampal administration of crotamine isolated from Crotalus whole venom. Adult rats received an intrahippocampal infusion of crotamine or vehicle and were euthanized 24 h or 21 days after infusion. Plasma and brain tissue were collected for biochemical analysis. Complete blood count, creatinine, urea, glutamic oxaloacetic transaminase (GOT, glutamic pyruvic transaminase (GPT, creatine-kinase (CK, creatine kinase-muscle B (CK-MB and oxidative parameters (assessed by DNA damage and micronucleus frequency in leukocytes, lipid peroxidation and protein carbonyls in plasma and brain were quantified. Unpaired and paired t-tests were used for comparisons between saline and crotamine groups, and within groups (24 h vs. 21 days, respectively. After 24 h crotamine infusion promoted an increase of urea, GOT, GPT, CK, and platelets values (p ≤ 0.01, while red blood cells, hematocrit and leukocytes values decreased (p ≤ 0.01. Additionally, 21 days after infusion crotamine group showed increased creatinine, leukocytes, TBARS (plasma and brain, carbonyl (plasma and brain and micronucleus compared to the saline-group (p ≤ 0.01. Our findings show that crotamine infusion alter hematological parameters and cardiac markers, as well as oxidative parameters, not only in the brain, but also in the blood, indicating a systemic pro-inflammatory and toxicological activity. A further scientific attempt in terms of preserving the beneficial activity over toxicity is required.

  12. The adrenergic retulation of the cardiovascular system in the South American rattlesnake, Crotalus durissus

    DEFF Research Database (Denmark)

    Galli, G.L.J.; Jensen, Nini Skovgaard; Abe, A.S.

    2007-01-01

    The present study investigates adrenergic regulation of the systemic and pulmonary circulations of the anaesthetised South American rattlesnake, Crotalus durissus. Haemodynamic measurements were made following bolus injections of adrenaline and adrenergic antagonists administered through a systemic...... arterial catheter. Adrenaline caused a marked systemic vasoconstriction that was abolished by phentolamine, indicating this response was mediated through α-adrenergic receptors. Injection of phentolamine gave rise to a pronounced vasodilatation (systemic conductance (Gsys) more than doubled), while...... injection of propranolol caused a systemic vasoconstriction, pointing to a potent α-adrenergic, and a weaker β-adrenergic tone in the systemic vasculature of Crotalus. Overall, the pulmonary vasculature was far less responsive to adrenergic stimulation than the systemic circulation. Adrenaline caused...

  13. Isolation and characterization of delta toxin from the venom of Crotalus durissus terrificus; Isolamento e caracterizacao da delta toxina do veneno de Crotalus durissus terrificus

    Energy Technology Data Exchange (ETDEWEB)

    Campos, Lucelia de Almeida

    2006-07-01

    The Crotalus durissus terrificus venom has been so far described as being of low complexity, with four major components described: convulxin, gyroxin, crotoxin and crotamine. In recent studies, other components of this venom were characterized as, for example, an analgesic factor. In 1980, Vital Brazil predicted the existence of a toxin which could be involved in platelet aggregation, and named it delta toxin. However, this toxin has never been isolated or characterized. The aim of the present work was to purify and characterize this toxin. After FPLC size exclusion chromatography followed by reverse phase HPLC, an homogeneous fraction was obtained, with a molecular weight of 14,074.92 Da. When analyzed by SOS-PAGE, this toxin presented an anomalous behavior, with a molecular weight of 14 kDa, while in 2D gels, spots around 40 kDa and with an isoelectrical point between 4 and 5 were observed suggesting isoforms with glicosilation microheterogeneity. After trypsin digestion, the fragments were submitted to the swissprot databank showing high homology (43% coverage, 15 matching peptides) with trocarin, a prothrombin activator from Tropidechis carinatus. These data were further confirmed by aminoacid analysis. The toxin was tested for its ability to activate factor II and X using synthetic substrates. Our data indicate a direct activation of factor X. The same toxin also behaved as a potent direct platelet aggregation activator on washed platelets. Assays with specific inhibitors indicate that neither metalloproteinase, nor serinoproteinase or t lectin domains are involved in the aggregating activity, since EDTA, benzamidin and D-galactose did not inhibit the toxin. In the present work, we were able to identify, purify and characterize a new toxin from the brazilian rattlesnake. It behaved as predicted by Vital-Brazil and displayed direct factor X activating properties, also inducing platelet aggregation, even at low concentrations. Our data also indicate that it is

  14. Antropometria, maturação sexual e idade da menarca de acordo com o nível socioeconômico de meninas escolares de Cascavel (PR Antropometry, sexual maturation and menarcheal age according to socioeconomic status of schoolgirls from Cascavel (PR

    Directory of Open Access Journals (Sweden)

    Everton Paulo Roman

    2009-01-01

    Full Text Available OBJETIVO: Avaliar a idade da menarca e a antropometria de meninas escolares de diferentes níveis socioeconômicos em Cascavel (Estado do Paraná -Região Sul do Brasil. MÉTODOS: Estudo transversal realizado no ano de 2006 com 2.761 meninas em 27 escolas públicas e particulares. A idade da menarca foi obtida pelo método status quo. Para a avaliação da maturação sexual foi realizada a autoavaliação de acordo com os critérios descritos por Tanner. O nível socioeconômico foi obtido por meio do questionário da ABEP (2003. Avaliou-se o peso e a estatura, obtendo-se o índice de massa corporal (IMC. Foram utilizados a análise estatística descritiva e o teste de Mann-Whitney, com nível de significância de 5%. RESULTADOS: A idade da menarca geral foi 12,2±1,2 anos e 12,1±1,1; 12,3±1,2 e 12,4±1,2 anos para os tercis alto, médio e baixo de nível socioeconômico, respectivamente. Foram constatados valores superiores para as meninas pós-menarca no peso e na estatura dos 10 aos 17 anos de idade e no IMC dos 11 aos 14 anos de idade. CONCLUSÕES: As meninas de nível socioeconômico alto apresentaram idade da menarca em idade inferior aos demais níveis socioeconômicos. Embora estas diferenças tenham sido observadas em níveis socioeconômicos distintos, biologicamente foram consideradas pequenas.OBJECTIVE: To evaluate age of menarche and anthropometric data of schoolgirls of different socioeconomic levels in Cascavel, state of Parana, in Southern Brazil. METHODS: A cross-sectional study was carried out in 2006 with 2,761 girls evaluated in 27 private and public schools. Age at menarche was obtained by the status quo method. Sexual maturity was assessed by self evaluation, according to Tanner's criteria. Socioeconomic level was obtained through the ABEP (2003 questionnaire. Data of weight, stature and Body Mass Index (BMI were obtained. Data were analyzed by descriptive statistics and Mann-Whitney test at 5% significance. RESULTS: Age

  15. Formas de uso medicinal da babosa e camomila pela população urbana de Cascavel, Estado do Paraná - doi: 10.4025/actascihealthsci.v32i2.6446 Medicinal use of the Aloe and Chamomile for the urban population of Cascavel, Paraná State - doi: 10.4025/actascihealthsci.v32i2.6446

    Directory of Open Access Journals (Sweden)

    Claudia Tatiana Araujo Cruz-Silva

    2010-09-01

    Full Text Available A etnobotânica se caracteriza por buscar entender a relação entre as plantas e o homem, podendo-se através desta conhecer a utilização das plantas medicinais como forma de tratamento. O objetivo deste trabalho foi realizar um levantamento sobre as formas de utilização da Babosa (Aloe vera L. e da Camomila (Matricaria chamomilla L.. A coleta de dados envolveu entrevistas de forma aleatória a 400 pessoas, através de um questionário semi-estruturado, no município de Cascavel, Estado do Paraná. Observou-se que 65% da população utilizam à babosa ou a camomila na cura ou alívio de doenças. A maioria dos entrevistados possui renda de 2 a 4 salários mínimos (60,25%, idade entre 28 a 38 anos (30,75%, tendo concluído o ensino médio (33,75%. O principal motivo pelo qual a população se utiliza de plantas medicinais é por ser natural (71,84%. A forma de preparo mais freqüente da camomila foi por infusão (63,38%, utilizando as flores (92%. Para a babosa 100% utilizam suas folhas na forma de cataplasma (43,88%. Apenas 3% da população relataram ter apresentado algum tipo de reação adversa, durante o período de utilização. Conclui-se que o uso destas plantas pela população é freqüente, sendo um recurso adicional ao uso de medicamentos.The ethnobotany, which is characterized by look for to understand the relationship between the plants and the man, being been able through this to know the use of the medicinal plants as treatment. The objective of this work was to accomplish a rising on the forms of use of the Aloe (Aloe vera L. and of the Chamomile (Matricaria chamomilla L.. The collection of data involved interviews in a random way to a sample of 400 people through a semi-structured questionnaire, in the city of Cascavel, Paraná State. It was observed that 65% of the population use to aloe or the chamomile in the cure or relief of diseases. Most of the interviewees possesses income from 2 to 4 minimum wages (60.25%, age

  16. Estudo sobre a evasão de pacientes em psicoterapia oferecida pela clínica escola da Unipan/Anhanguera de Cascavel-PR por membros da comunidade de baixa renda

    OpenAIRE

    Siqueira, Vinicius Reis de; Unipan - Anhanguera

    2013-01-01

    Considerando-se a importância da psicoterapia individual para os psicólogos, e o fato de existir um número consideravelmente alto de desistências da psicoterapia por pessoas que possuem uma baixa renda, este estudo buscou verificar os possíveis motivos que levam a evasão desta população na clinica escola da UNIPAN/Anhanguera de Cascavel/PR. Para tanto houve um processo de seleção dos atendimentos realizados no ano de 2010 para alcançar esta população (de baixa renda que desistiu em até 7 enco...

  17. Irradiação global, direta e difusa, para a região de Cascavel, Estado do Paraná - DOI: 10.4025/actascitechnol.v28i1.1288

    Directory of Open Access Journals (Sweden)

    Patrícia Aparecida de Oliveira Drechmer

    2006-03-01

    Full Text Available O presente trabalho apresenta um estudo sobre os níveis das irradiações global, direta e difusa incidentes na superfície terrestre, a irradiação incidente no topo da atmosfera e a insolação relativa mensal, para a cidade de Cascavel-PR. As componentes da radiação solar foram monitoradas entre janeiro de 2001 e fevereiro de 2003 na Estação Experimental Agrometeorológica da Unioeste, Cascavel, Estado do Paraná. Na medição da radiação global foi utilizado um piranômetro KIPP & ZONEN-CM3 e para medir a componente direta foi utilizado um pireliômetro EPPLEY-NIP acoplado a um rastreador solar. A radiação difusa foi calculada pela diferença entre as radiações global e direta. Os níveis médios mensais das irradiações global, direta e difusa, foram respectivamente, 51,8%, 31,7% e 20,17% da irradiação incidente no topo da atmosfera. O índice de insolação relativa apresentou valor máximo em agosto (61,43% e mínimo em fevereiro (34,35%, períodos caracterizados respectivamente por dias de céu limpo e nublados no hemisfério sul

  18. Observations on white and yellow venoms from an individual southern Pacific rattlesnake (Crotalus viridis helleri).

    Science.gov (United States)

    Johnson, E K; Kardong, K V; Ownby, C L

    1987-01-01

    Biochemical differences in white and yellow venoms produced in the separate venom glands of an individual southern Pacific rattlesnake (Crotalus viridis helleri) were investigated. Compared to the yellow venom, the white venom contained fewer low molecular weight components and was considerably less toxic. Although the exact LD50 was not determined, the white venom did not produce toxic effects in mice when injected i.v. at concentrations up to 10 mg/kg. The i.v. LD50 of the yellow venom was approximately 1.6 mg/kg. Both white and yellow venoms had hemorrhagic activity, but the white venom caused less intradermal hemorrhage in mice. No L-amino acid oxidase activity was measured in the white venom and protease and phospholipase A2 activities of the white venom were much less than in the yellow venom. The white and yellow venoms both produced myonecrosis at 1, 3 and 24 hr after i.m. injection into mice, however, there were some qualitative differences in the myonecrosis produced. When the venom samples were reacted against Wyeth's polyvalent (Crotalidae) antivenom using immunodiffusion, three precipitin bands formed against the yellow venom, whereas only one formed against the white venom. When reacted against an antiserum to myotoxin alpha from C. viridis viridis venom, both the white and yellow venoms produced one precipitin band each.

  19. Mechanisms controlling venom expulsion in the western diamondback rattlesnake, Crotalus atrox.

    Science.gov (United States)

    Young, Bruce A; Kardong, Kenneth V

    2007-01-01

    Although many studies have documented variation in the amount of venom expended during bites of venomous snakes, the mechanistic source of this variation remains uncertain. This study used experimental techniques to examine how two different features of the venom delivery system, the muscle surrounding the venom gland (the Compressor Glandulae in the rattlesnake) and the fang sheath, could influence venom flow in the western diamondback rattlesnake, Crotalus atrox. Differential contraction of the Compressor Glandulae explained only approximately 30% of the variation in venom flow. Lifting (compression) of the fang sheath as occurs during a normal strike produced marked increases in venom flow; these changes were closely correlated and exceed in magnitude by almost 10 x those recorded from the Compressor Glandulae alone. These results suggest that variation in these two aspects of the venom delivery system--both in terms of magnitude and temporal patterning--explain most of the observed variation in venom injection. The lack of functional or mechanical links between the Compressor Glandulae and the fang sheath, and the lack of skeletal or smooth muscle within the fang sheath, make it unlikely that variation in venom flow is under direct neural control. Instead, differential venom injection results from differences in the pressurization by the Compressor Glandulae, the gate keeping effects of the fang sheath and enclosed soft-tissue chambers, and by differences in the pressure returned by peripheral resistance of the target tissue.

  20. Pro- and Anti-Inflammatory Cytokines Release in Mice Injected with Crotalus durissus terrificus Venom

    Directory of Open Access Journals (Sweden)

    A. Hernández Cruz

    2008-01-01

    Full Text Available The effects of Crotalus durissus terrificus venom (Cdt were analyzed with respect to the susceptibility and the inflammatory mediators in an experimental model of severe envenomation. BALB/c female mice injected intraperitoneally presented sensibility to Cdt, with changes in specific signs, blood biochemical and inflammatory mediators. The venom induced reduction of glucose and urea levels and an increment of creatinine levels in serum from mice. Significant differences were observed in the time-course of mediator levels in sera from mice injected with Cdt. The maximum levels of IL-6, NO, IL-5, TNF, IL-4 and IL-10 were observed 15 min, 30 min, 1, 2 and 4 hours post-injection, respectively. No difference was observed for levels of IFN-γ. Taken together, these data indicate that the envenomation by Cdt is regulated both pro- and anti-inflammatory cytokine responses at time-dependent manner. In serum from mice injected with Cdt at the two first hours revealed of pro-inflammatory dominance. However, with an increment of time an increase of anti-inflammatory cytokines was observed and the balance toward to anti-inflammatory dominance. In conclusion, the observation that Cdt affects the production of pro- and anti-inflammatory cytokines provides further evidence for the role played by Cdt in modulating pro/anti-inflammatory cytokine balance.

  1. Biochemical and biological evaluation of gyroxin isolated from Crotalus durissus terrificus venom

    Directory of Open Access Journals (Sweden)

    LC Barros

    2011-01-01

    Full Text Available Gyroxin, a thrombin-like enzyme isolated from Crotalus durissus terrificus venom and capable of converting fibrinogen into fibrin, presents coagulant and neurotoxic activities. The aim of the present study was to evaluate such coagulant and toxic properties. Gyroxin was isolated using only two chromatographic steps - namely gel filtration (Sephadex G-75 and affinity (Benzamidine Sepharose 6B - resulting in a sample of high purity, as evaluated by RP-HPLC C2/C18 and electrophoretic analysis that showed a molecular mass of 30 kDa. Gyroxin hydrolyzed specific chromogenic substrates, which caused it to be classified as a serine proteinase and thrombin-like enzyme. It was stable from pH 5.5 to 8.5 and inhibited by Mn²+, Cu²+, PMSF and benzamidine. Human plasma coagulation was more efficient at pH 6.0. An in vivo toxicity test showed that only behavioral alterations occurred, with no barrel rotation. Gyroxin was not able to block neuromuscular contraction in vitro, which suggests that its action, at the studied concentrations, has no effect on the peripheral nervous system.

  2. American rattlesnake (Crotalus durissus terrificus bite accidents in dogs in Argentina

    Directory of Open Access Journals (Sweden)

    Koscinczuk P.

    2000-01-01

    Full Text Available The symptomatology and treatment of two dogs bitten by Crotalus durissus terrificus are described. Neurological signs were present few minutes after the accident with local anesthesia and ataxia of the affected limb and neurotoxic fascia. Alanine aminotransferase (ALT, aspartate aminotransferase (AST, creatinine kinase (CK, lactate dehydrogenase (LDH and calcium were evaluated in an attempt to investigate muscle damage. Renal failure was not observed but some alterations were detected in urine. Urine density was low and the urinary sediment contained granular clumps and small round cells. Muscle samples were obtained from both legs for histopathological study, showing edema and isolated necrotic fibers. Both dogs received treatment within four hours after the accident by intravenous route. The antivenom was administered diluted in 250ml of Ringer solution in a dose enough to inactivate more than 8mg of venom. Dexamethasone was applied previously to the antivenom. Clinical evolution was good and both patients were in good health condition on the second day after the accident.

  3. Pulmonary mechanic and lung histology injury induced by Crotalus durissus terrificus snake venom.

    Science.gov (United States)

    Nonaka, Paula Naomi; Amorim, César Ferreira; Paneque Peres, Ana Claudia; E Silva, César Augusto Melo; Zamuner, Stella R; Ribeiro, Wellington; Cogo, José Carlos; Vieira, Rodolfo P; Dolhnikoff, Marisa; de Oliveira, Luis Vicente Franco

    2008-06-01

    In the present work we investigated the effects of Crotalus durissus terrificus venom (CdtV) on the pulmonary mechanic events [static and dynamic elastance, resistive (DeltaP1) and viscoelastic pressures (DeltaP2)] and histology after intramuscular injection of saline solution (control) or venom (0.6 microg/g). The static and dynamic elastance values were increased significantly after 3 h of venom inoculation, but were reduced at control values in the other periods studied. The DeltaP1 values that correspond to the resistive properties of lung tissue presented a significant increase after 6h of CdtV injection, reducing to basal levels 12h after the venom injection. In DeltaP2 analysis, correspondent to viscoelastic components, an increase occurred 12 h after the venom injection, returning to control values at 24 h. CdtV also caused an increase of leukocytes recruitment (3-24 h) to the airways wall as well as to the lung parenchyma. In conclusion, C. durissus terrificus rattlesnake venom leads to lung injury which is reverted, after 24 h of inoculation.

  4. Occurrence of Cryptosporidium (Apicomplexa, Cryptosporidiidae in Crotalus durissus terrificus (Serpentes, Viperidae in Brazil

    Directory of Open Access Journals (Sweden)

    Karasawa Andréa Satie Matsubara

    2002-01-01

    Full Text Available The objective of the present study was to investigate the prevalence of Cryptosporidium (Apicomplexa, Cryptosporidiidae in the snake Crotalus durissus terrificus (Serpentes, Viperidae. Fifty animals were evaluated for the presence of oocysts of Cryptosporidium sp. at the time of arrival and 30 and 60 days later. Intestinal washings with saline solution (1% body weight, fecal samples, and organ scrapings were collected during the study. Oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by a density gradient centrifugation technique. Smears were made with the sediment and submitted to modified acid-fast and auramine-rhodamine staining. Cryptosporidium-positive smears were used as controls for the experimental findings. The overall prevalence of Cryptosporidium sp. oocysts was 14%. Among the positive snakes, oocysts were detected only in the intestinal washing in two specimens, only in the feces in four specimens, and in both materials at least once in one specimen. The positive snakes were predominantly from Santa Maria da Serra city State of São Paulo (57.1%. We also observed that all of the examinations that presented positive results were obtained at least 27 days after the capture of the animals.

  5. Clinical management of babesiosis in dogs with homeopathic Crotalus horridus 200C.

    Science.gov (United States)

    Chaudhuri, S; Varshney, J P

    2007-04-01

    Homeopathic Crotalus horridus 200C was evaluated in 13 clinical cases of babesiosis in dogs, compared with another 20 clinical cases treated with diminazine. Babesiosis is an important tropical tick-borne haemoprotozoan disease in dogs clinically manifested by anorexia, dehydration, temperature, dullness/depression, diarrhoea/constipation, pale mucosa, hepatomegaly, vomiting/nausea, splenomegaly, distended abdomen/ascites, yellow coloured urine, emaciation/weight loss, and occular discharge. The diagnosis of babesiosis was based on cytological evidence of Babesia gibsoni in freshly prepared blood smears. The dogs were treated with oral C. horridus 200C, 4 pills four times daily for 14 days (n=13) or diminazine aceturate 5 mg/kg single intramuscularly dose (n=20). All the dogs were administered 5% Dextrose normal saline at 60 ml/kg intravenously for 4 days. Initial clinical scores were similar in both groups and showed similar progressive improvement with the two treatments over 14 days. Parasitaemia also improved in both groups, but haematological values showed no change. No untoward reactions were observed. It appears that C. horridus is as effective in causing clinical recovery in moderate cases of canine babesiosis caused by Babesia gibsoni as the standard drug diminazine. Large scale randomized trials are indicated for more conclusive results.

  6. Responses of infrared-sensitive tectal units of the pit viper Crotalus atrox to moving objects.

    Science.gov (United States)

    Kaldenbach, Felix; Bleckmann, Horst; Kohl, Tobias

    2016-06-01

    Rattlesnakes perceive IR radiation with their pit organs. This enables them to detect and strike towards warm-blooded prey even in the dark. In addition, the IR sense allows rattlesnakes to find places for thermoregulation. Animate objects (e.g., prey) tend to move and thus cause moving IR images across the pit membrane. Even when an object is stationary, scanning head movements of rattlesnakes will result in moving IR images across the pit membrane. We recorded the neuronal activity of IR-sensitive tectal neurons of the rattlesnake Crotalus atrox while stimulating the snakes with an IR source that moved horizontally at various velocities. As long as object velocity was low (angular velocity of ~5°/s) IR-sensitive tectal neurons hardly showed any responses. With increasing object velocity though, neuronal activity reached a maximum at ~50°/s. A further increase in object velocity up to ~120°/s resulted in a slight decrease of neuronal activity. Our results demonstrate the importance of moving stimuli for the snake's IR detection abilities: in contrast to fast moving objects, stationary or slowly moving objects will not be detected when the snake is motionless, but might be detected by scanning head movements.

  7. Short Note. Chemosensory response in stunted prairie rattle snakes Crotalus viridis viridis

    Directory of Open Access Journals (Sweden)

    Anthony J. SAVIOLA, David CHISZAR, Hobart M. SMITH,Stephen P. MACKESSY

    2013-04-01

    Full Text Available Rattlesnakes use chemical stimuli in ambush site selection and for relocation of envenomated prey through strike-induced chemosensory searching. Shifts in responsiveness to prey chemicals have been documented in many snakes, and often correlate with prey commonly taken as snakes increase in age and size as well as geographical locations of the species. For instance, neonate rattlesnakes that prey primarily on ectotherms responded most strongly to chemical cues of commonly taken lizard prey, whereas adult rattlesnakes that prey primarily on small mammals responded significantly to chemical cues of commonly taken rodents. In the current study, 11 Prairie Rattlesnakes Crotalus viridis viridis which were classified as large neonates based on measures of snout-vent length (SVL and body mass, yet chronologically were at or near adulthood, were tested for their responsiveness to chemical extracts of natural and non-natural prey items. Although the snakes had eaten only neonate lab mice (Mus musculus, they responded significantly more to chemical cues of natural prey items and particularly to chemical cues of prey normally taken by subadults (Peromyscus mice and Sceloporus lizard. These results suggest that ontogenetic shifts in responsiveness to natural prey chemical cues are innately programmed and are not based on body size or feeding experience in C. v. viridis. This does not imply, however, that growth and experience are without effects, especially with novel prey or rare prey that have experienced recent population expansion [Current Zoology 59 (2: 175-179, 2013].

  8. Envenomations by Bothrops and Crotalus snakes induce the release of mitochondrial alarmins.

    Directory of Open Access Journals (Sweden)

    Irene Zornetta

    Full Text Available Skeletal muscle necrosis is a common manifestation of viperid snakebite envenomations. Venoms from snakes of the genus Bothrops, such as that of B. asper, induce muscle tissue damage at the site of venom injection, provoking severe local pathology which often results in permanent sequelae. In contrast, the venom of the South American rattlesnake Crotalus durissus terrificus, induces a clinical picture of systemic myotoxicity, i.e., rhabdomyolysis, together with neurotoxicity. It is known that molecules released from damaged muscle might act as 'danger' signals. These are known as 'alarmins', and contribute to the inflammatory reaction by activating the innate immune system. Here we show that the venoms of B. asper and C. d. terrificus release the mitochondrial markers mtDNA (from the matrix and cytochrome c (Cyt c from the intermembrane space, from ex vivo mouse tibialis anterior muscles. Cyt c was released to a similar extent by the two venoms whereas B. asper venom induced the release of higher amounts of mtDNA, thus reflecting hitherto some differences in their pathological action on muscle mitochondria. At variance, injection of these venoms in mice resulted in a different time-course of mtDNA release, with B. asper venom inducing an early onset increment in plasma levels and C. d. terrificus venom provoking a delayed release. We suggest that the release of mitochondrial 'alarmins' might contribute to the local and systemic inflammatory events characteristic of snakebite envenomations.

  9. Directional sensitivity in the thermal response of the facial pit in western diamondback rattlesnakes (Crotalus atrox).

    Science.gov (United States)

    Kohl, Tobias; Colayori, Samantha E; Westhoff, Guido; Bakken, George S; Young, Bruce A

    2012-08-01

    Recent work published in the accompanying paper used a combination of 3D morphological reconstruction to define optical spread functions and heat transfer physics to study how external heat energy would reach the sensory membrane within the facial pit of pitvipers. The results from all of the species examined indicated asymmetric directional sensitivity, e.g. the pit would preferentially respond to stimuli located below and behind the snake. The present study was intended as a test of these findings through a quantitative neurophysiological analysis of directional sensitivity in the facial pit of the western diamondback rattlesnake, Crotalus atrox. An infrared emitter was positioned through a coordinate system (with varying angular orientations and distances) and the response it evoked measured through neurophysiological recordings of a trigeminal nerve branch composed of the afferents from the sensory membrane of the facial pit. Significant differences were found in the strength of the membrane's neural response to a constant stimulus presented at different orientations (relative to the facial pit opening) and over different distances. The peak sensitivity (at 12 deg above and 20 deg in front of the facial pit opening) was in good agreement with the predicted directional sensitivities based on optical spread functions and 3D topography. These findings support the hypothesis that the topography, and functional performance, of the facial pit has undergone an adaptive radiation within the pit vipers, and that differences in the behavioral ecology of the pit vipers (i.e. terrestrial versus arboreal) are reflected within the facial pits.

  10. Natural variation in steroid hormone profiles of male Timber Rattlesnakes, Crotalus horridus, in northwest Arkansas.

    Science.gov (United States)

    Lind, Craig M; Beaupre, Steven J

    2014-09-15

    We describe the seasonal profile of circulating steroid hormones (testosterone and corticosterone) in relation to the breeding season in free ranging male Timber Rattlesnakes, Crotalus horridus, over the course of three active seasons. In addition, we examine variation in steroid concentrations across years and in relation to body condition. We found that seasonal profiles of plasma testosterone were different compared to other crotalines with similar mating patterns. Concentrations of testosterone were elevated above baseline in the three months leading up to the single late summer breeding season. Testosterone peaked in July at the onset of the breeding season and dropped to baseline during the peak months of breeding (August and September). Testosterone concentrations also varied annually. Although the exact cause of annual variation could not be established, our results indicate that weather patterns may have driven observed differences. Testosterone concentrations were positively related to body condition, indicating that testosterone production is modulated according to energetic status (particularly in the two months prior to the breeding season). Corticosterone did not vary seasonally or with any measured variable, a result similar to other studied crotalines. Our results highlight the importance of long-term descriptive studies of the regulatory mechanisms that underlie behavior and physiology in diverse taxa, as these mechanisms can vary greatly within and among populations and are valuable in elucidating the intrinsic and extrinsic sources of such variation.

  11. Venom variation in hemostasis of the southern Pacific rattlesnake (Crotalus oreganus helleri): isolation of hellerase.

    Science.gov (United States)

    Salazar, Ana Maria; Guerrero, Belsy; Cantu, Bruno; Cantu, Esteban; Rodríguez-Acosta, Alexis; Pérez, John C; Galán, Jacob A; Tao, Andy; Sánchez, Elda E

    2009-04-01

    Envenomations by the southern Pacific rattlesnake (Crotalus oreganus helleri) are the most common snakebite accidents in southern California. Intraspecies venom variation may lead to unresponsiveness to antivenom therapy. Even in a known species, venom toxins are recognized as diverse in conformity with interpopulational, seasonal, ontogenetic and individual factors. Five venoms of individual C. oreganus helleri located in Riverside and San Bernardino counties of southern California were studied for their variation in their hemostatic activity. The results demonstrated that Riverside 2 and San Bernardino 1 venoms presented the highest lethal activity without hemorrhagic activity. In contrast, San Bernardino 2 and 3 venoms had the highest hemorrhagic and fibrinolytic activities with low lethal and coagulant activities. Riverside 1, Riverside 2 and San Bernardino 1 venoms presented a significant thrombin-like activity. San Bernardino 2 and 3 venoms presented an insignificant thrombin-like activity. In relation to the fibrinolytic activity, San Bernardino 3 venom was the most active on fibrin plates, which was in turn neutralized by metal chelating inhibitors. These results demonstrate the differences amongst C. oreganus helleri venoms from close localities. A metalloproteinase, hellerase, was purified by anionic and cationic exchange chromatographies from San Bernardino 3 venom. Hellerase exhibited the ability to break fibrin clots in vitro, which can be of biomedically importance in the treatment of heart attacks and strokes.

  12. Comprometimento respiratório secundário a acidente ofídico crotálico (Crotalus durissus Respiratory abnormalities following Crotalus durissus snakebite

    Directory of Open Access Journals (Sweden)

    Carlos Faria Santos Amaral

    1991-08-01

    Full Text Available São analisados três pacientes que apresentaram comprometimento da função respiratória após acidente por Crotalus durissus. As manifestações respiratórias surgiram nas primeiras 48 horas após a picada do ofídio e consistiram de dispnéia, taquipnéia, uso da musculatura acessória da respiração (casos 1 e 2 e batimento das aletas nasais (caso 2. Dois pacientes (casos 1 e 2 apresentaram insuficiência respiratória aguda. O diagnóstico desta complicação no caso 1 foi clínico pois o paciente apresentou apnéia. O paciente do caso 2, 24 horas após o acidente ofídico apresentou dificuldade respiratória intensa e períodos de apnéia sendo intubado, permanecendo em respiração espontânea. Houve agravamento dos sinais clínicos de insuficiência respiratória e a determinação de pH e gases do sangue arterial mostrou em relação ao exame inicial elevação da pressão parcial de gás carbônico (40 mmHg para 50,3 mm Hg caracterizando insuficiência ventilatória aguda. Ambos foram tratados com emprego de ventilação artificial mecânica, tendo o paciente do caso 1 permanecido no ventilador durante 33 dias e o do caso 2 durante 15 dias. Ambos desenvolveram insuficiência renal aguda, necessitaram de diálise peritoneal e recuperaram a função renal. A paciente do caso 3, apesar dos sintomas e sinais de comprometimento respiratório não apresentou alterações do pH e gases arteriais. Espirometria realizada 58 horas após o acidente mostrou capacidade vital forçada (CVF e volume espirado no primeiro segundo (VEF1 abaixo do previsto (60 e 67% respectivamente. As espirometrias realizadas nos dias subseqüentes evidenciaram melhora progressiva destes parâmetros. No 10º dia após o acidente constatou-se aumento de 20% da CVF e de 17% do VEF1 comparativamente ao exame inicial. A relação entre VEF1 e a CVF manteve-se praticamente inalterada e em valores próximos ao previsto, caracterizando distúrbio ventilatório do tipo

  13. Diversity-dependent cladogenesis throughout western Mexico: Evolutionary biogeography of rattlesnakes (Viperidae: Crotalinae: Crotalus and Sistrurus).

    Science.gov (United States)

    Blair, Christopher; Sánchez-Ramírez, Santiago

    2016-04-01

    Rattlesnakes (Crotalus and Sistrurus) represent a radiation of approximately 42 species distributed throughout the New World from southern Canada to Argentina. Interest in this enigmatic group of snakes continues to accrue due, in part, to their ecomorphological diversity, contributions to global envenomations, and potential medicinal importance. Although the group has garnered substantial attention from systematists and evolutionary biologists for decades, little is still known regarding patterns of lineage diversification. In addition, few studies have statistically quantified broad-scale biogeographic patterns in rattlesnakes to ascertain how dispersal occurred throughout the New World, particularly among the different major biomes of the Americas. To examine diversification and biogeographic patterns in this group of snakes we assemble a multilocus data set consisting of over 6700bp encompassing three nuclear loci (NT-3, RAG-1, C-mos) and seven mitochondrial genes (12S, 16S, ATPase6, ATPase8, ND4, ND5, cytb). Fossil-calibrated phylogenetic and subsequent diversification rate analyses are implemented using maximum likelihood and Bayesian inference, to examine their evolutionary history and temporal dynamics of diversity. Based on ancestral area reconstructions we explore dispersal patterns throughout the New World. Cladogenesis occurred predominantly during the Miocene and Pliocene with only two divergences during the Pleistocene. Two different diversification rate models, advocating diversity-dependence, are strongly supported. These models indicate an early rapid radiation followed by a recent speciation rate decline. Biogeographic analyses suggest that the high elevation pine-oak forests of western Mexico served as a major speciation pump for the majority of lineages, with the desert biome of western North America colonized independently at least twice. All together, these results provide evidence for rapid diversification of rattlesnakes throughout the

  14. Tracing an invasion: landbridges, refugia, and the phylogeography of the Neotropical rattlesnake (Serpentes: Viperidae: Crotalus durissus).

    Science.gov (United States)

    Wüster, Wolfgang; Ferguson, Julia E; Quijada-Mascareñas, J Adrian; Pook, Catharine E; Salomão, Maria da Graça; Thorpe, Roger S

    2005-04-01

    Abstract Pleistocene fragmentation of the Amazonian rainforest has been hypothesized to be a major cause of Neotropical speciation and diversity. However, the role and even the reality of Pleistocene forest refugia have attracted much scepticism. In Amazonia, previous phylogeographical studies have focused mostly on organisms found in the forests themselves, and generally found speciation events to have predated the Pleistocene. However, molecular studies of open-formation taxa found both north and south of the Amazonian forests, probably because of vicariance resulting from expansion of the rainforests, may provide novel insights into the age of continuous forest cover across the Amazon basin. Here, we analyse three mitochondrial genes to infer the phylogeography of one such trans-Amazonian vicariant, the Neotropical rattlesnake (Crotalus durissus), which occupies primarily seasonal formations from Mexico to Argentina, but avoids the rainforests of Central and tropical South America. The phylogeographical pattern is consistent with gradual dispersal along the Central American Isthmus, followed by more rapid dispersal into and across South America after the uplift of the Isthmus of Panama. Low sequence divergence between populations from north and south of the Amazon rainforest is consistent with mid-Pleistocene divergence, approximately 1.1 million years ago (Ma). This suggests that the Amazonian rainforests must have become fragmented or at least shrunk considerably during that period, lending support to the Pleistocene refugia theory as an important cause of distribution patterns, if not necessarily speciation, in Amazonian forest organisms. These results highlight the potential of nonforest species to contribute to an understanding of the history of the Amazonian rainforests themselves.

  15. Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: Evidence of a broad arsenal of toxins.

    Science.gov (United States)

    Viala, Vincent Louis; Hildebrand, Diana; Fucase, Tamara Mieco; Sciani, Juliana Mozer; Prezotto-Neto, José Pedro; Riedner, Maria; Sanches, Leonardo; Nishimura, Paula Juliana; Oguiura, Nancy; Pimenta, Daniel Carvalho; Schlüter, Hartmut; Betzel, Christian; Arni, Raghuvir Krishnaswami; Spencer, Patrick Jack

    2015-12-01

    The investigation of venoms has many clinical, pharmacological, ecological and evolutionary outcomes. The Crotalus spp. venom can cause hemorrhage, neurotoxicity, myotoxicity, coagulopathy and hypotension. Although neurotoxicity and hemorrhage usually does not occur for the same species, the rare Venezuelan species Crotalus vegrandis presents both characteristic. Different from the other species it has a restricted ecological niche and geographical distribution. Nevertheless, it has a raising medical importance as this rattlesnake population is increasing. Few works describe its neurotoxic and hemorrhagic features, but other toxins might play an important role in envenomation. We combined proteomic methods to identify for the first time the main components of it venom: 2D SDS-PAGE and gel-filtration chromatography for protein mixture decomplexation; LC-MS(2) of low molecular mass fractions and tryptic peptides; bioinformatic identification of toxin families and specific protein species based on unique peptide analysis and sequence database enriched with species-specific venom gland transcripts; and finally polyclonal anti-crotamine Western-blotting. Our results point to a broad arsenal of toxins in C. vegrandis venom: PIII and PII metalloproteases, crotoxin subunits, other phospholipases, isoforms of serine proteases and lectins, l-amino-acid oxidase, nerve growth factor, as well as other less abundant toxins.

  16. Amino acid sequence and disulfide bond assignment of myotoxin a isolated from the venom of prairie rattlesnake (Crotalus viridis viridis)

    Energy Technology Data Exchange (ETDEWEB)

    Fox, J.W.; Elzinga, M.; Tu, A.T.

    1979-02-20

    The primary structure of myotoxin a, a myotoxin protein from the venom of the North American rattlesnake Crotalus viridis viridis, was determined and the position of the disulfide bonds assigned. The toxin was isolated, carboxymethylated, and cleaved by cyanogen bromide, and the resultant peptides were isolated. The cyanogen bromide peptides were subjected to amino acid sequence analysis. In order to assign the positions of the three disulfide bonds, the native toxin was cleaved sequentially with cyanogen bromide and trypsin. A two peptide unit connected by one disulfide bond was isolated and characterized, and a three-peptide unit connected by two disulfide bonds was isolated. One peptide in the three-peptide unit was identified as Cys-Cys-Lys. In order to establish the linkages between the peptides and Cys-Cys-Lys, one cycle of Edman degradation was carried out such that the Cys-Cys bond was cleaved. Upon isolation and analysis of the cleavage products, the disulfide bonds connecting the three peptides were determined. The positions of the disulfide bridges of myotoxin a were determined to be totally different from those of neurotoxins isolated from snake venoms. The sequence of myotoxin a was compared with the sequences of other snake venom toxins using the computer program RELATE to determine whether myotoxin a is similar to any other types of toxins. From the computer analysis, myotoxin a did not show any close relationship to other toxins except crotamine from the South American rattlesnake Crotalus durissus terrificus.

  17. Anti-platelet effect of cumanastatin 1, a disintegrin isolated from venom of South American Crotalus rattlesnake.

    Science.gov (United States)

    Da Silva, Manuel; Lucena, Sara; Aguilar, Irma; Rodríguez-Acosta, Alexis; Salazar, Ana M; Sánchez, Elda E; Girón, Maria E; Carvajal, Zoila; Arocha-Piñango, Carmen L; Guerrero, Belsy

    2009-03-01

    Disintegrins have been previously described in the venom of several snake families inhibiting signal transduction, cell-cell interactions, and cell-matrix interactions and may have therapeutic potential in heart attacks, thrombotic diseases, and cancers. This investigation describes the first disintegrin isolated from South American Crotalus venom (Venezuelan rattlesnake Crotalus durissus cumanensis), which inhibits platelet adhesion to matrix proteins. C. d. cumanensis crude venom was first separated on a Sephadex G-100 column into 4 fractions (SI to SIV). Crude venom and SIII fraction significantly diminished platelet adhesion to fibrinogen (Fg) and to fibronectin (Fn). Anti-adhesive SIII fraction was further separated by DEAE-Sephacel followed by C-18 reverse phase high performance liquid chromatography (HPLC). The platelet anti-adhesive fraction obtained was designated as cumanastatin-1. This disintegrin has a mass of 7.442 kDa as determined by mass spectrometry (MALDI-TOF/TOF) and pI of 8.5. Cumanastatin-1 also inhibited ADP-induced platelet aggregation with an IC(50) of 158 nM. However, it did not significantly inhibit collagen and thrombin-induced platelet aggregation. Cumanastatin-1 considerably inhibited anti-alpha(IIb)beta(3) integrin binding to platelets in a dose-dependent manner; however, it did not present any effect on the alpha(5)beta(1) integrin or on P-selectin.

  18. Espaço urbano e criminalidade violenta: análise da distribuição espacial dos homicídios no município de Cascavel/PR

    Directory of Open Access Journals (Sweden)

    Fernanda Pamplona Ramão

    2010-02-01

    Full Text Available A pesquisa buscou examinar e compreender a incidência desigual dos homicídios no perímetro urbano da cidade de Cascavel, no Paraná, entre os anos 2000 e 2006. Procurou-se entender porque esse tipo de crime variou de uma unidade administrativa para outra e a relação existente entre esse fenômeno e desigualdades socioeconômicas, de infra-estrutura e serviços urbanos. Partiu-se da discussão sobre o processo de urbanização desordenada no Brasil, que favoreceu a emergência e/ou a intensificação de diversas problemáticas, dentre as quais a da criminalidade violenta e a dos homicídios. A cidade de Cascavel apresenta altas taxas de homicídio, sendo que poucas unidades administrativas as concentram. Nesse contexto e com base nas explicações postuladas na literatura de referência, realizou-se uma pesquisa empírica sobre a incidência desigual dos homicídios no referido município e sua associação com indicadores de desigualdade. Utilizou-se a técnica estatística de Análise Exploratória de Dados Espaciais (AEDE, além de uma análise de sobreposição espacial de informações. Constatou-se que o fenômeno em estudo apresentou forte associação com as desigualdades ao longo do espaço urbano. Pode-se afirmar, portanto, que os cenários de "sobreposição de carências", conseqüentemente de baixa qualidade de vida da população residente, potencializaram a ocorrência de conflitos violentos que resultaram em morte.

  19. Congenital toxoplasmosis in South American children = Toxoplasmose congênita em crianças sul-americanas

    Directory of Open Access Journals (Sweden)

    Gómez-Marín, Jorge E.

    2010-01-01

    Conclusões: a toxoplasmosis congênita na América do Sul parece ser mais frequente e as crianças infectadas são mais sintomáticas do que na Europa e na América do Norte. A pesquisa sobre novas drogas e vacinas deve ser prioritária, para melhorar os indicadores de saúde nas crianças da América do Sul

  20. Lesões neurológicas na blastomicose sul-americana estudo anatomopatológico de 14 casos

    Directory of Open Access Journals (Sweden)

    Walter C. Pereira

    1965-06-01

    Full Text Available Several case reports of involvement of the nervous system by Paracoccidioides brasiliensis have been published, changing thus the concept that the nervous system would no be adequate to the proliferation of this fungus. In the material of the Department of Pathology of the University of São Paulo Medical School, collected up to September 1964, 145 cases of paracoccidioidomycosis were found. In 14 of them there were lesions of the nervous system, giving an incidence of 9,65%. This high incidence contradicts the frequent assumption that the involvement of the nervous system in cases of South American blastomycosis is exceptional. In our material most of the cases of neuroblastomycosis had not systemic lesions, 2 of them having isolated involvement of the nervous system. This fact does not agree with the frequent observation of patients with lesions in all organs but the nervous system. There are no pathophysiologic reasons to explain such contradiction, but it can be assumed that individual conditions, in some cases, make the nervous system vulnerable to the parasite. Of the 14 cases studied, 5 had lesions of the meninges and 9 had gra-nulomata of the brain. Of the 5 cases with meningitis, 3 had an isolated basilar leptomeningitis; in 1 case lesions of the pia-arachnoid of the brain convexity were also found, and in another one the spinal meninges were also involved as well the spinal roots. The granulomata of the brain were isolated in 5 cases and multiple in 4. The most frequent site of the lesions was the cerebral hemispheres (6 cases; in 2 cases the cerebellum was involved; the thalamus, pons and medulla were involved in one case each. This predominance of the hemispheric localization must be related to the larger blood supply of this parte of the nervous system, owing to the hematogenic route followed by the parasite. In our material no lesions of the spinal cord were found. However, no definitive conclusions can be drawn, as there was no systematic study of that parte of the nervous system. From a histopathologic viewpoint the meningeal inflammatory lesions were of the productive type, with a nodular or diffuse distribution. The characteristic cellular elements of this process are the gigantocytes of the Langhans type, the histiocytes, lymphocytes and plasmocytes, the two latter showing a predominant perivascular distribution. In the nodular forms there is a trend to the confluence of the granulomata and focal zones of caseous or gummous necrosis can be found. Arterites also can be found, causing degeneration of the nervous structures. The Paracoccidioides brasiliensis are generally abundantly found either free or phagocyted by histiocytes or gigantocytes. The inflammatory infiltrate grows along the Virchow-Robin's spaces, reaching the outer layers of the nervous parenchyma. The parenchymatous forms present a large central necrotic areas, with the characteristics of caseous or gummous necrosis, surrounded by histiocytes, gigantocytes, lymphocytes and plasmocytes. Fibroblasts and collagenous fibers surround and limit these granulomata of the encephalic parenchyma. Arterites are also observed and seem to play an importante role in the necrotic processes. The fungi are generally abundant, being found especially at the periphery of the necrotic areas or within the inflammatory tissues, free or phagocyted by histiocytes. In the nervous tissue neighboring the granulomata various degrees of degenerative processes of the neurons and glia are observed, as well as hyperplastic reaction of the oligodendroglia, microglia and astroglia. The inflammatory reaction of South American blastomycosis of the nervous system is not specific and, by itself, does not permit the etiologic diagnosis of the process. Only the finding of the parasite, with its characteristic forms, make it possible to establish the cause of the lesion.

  1. Integração nacional e sul-americana: velho debate, novas con(tradições

    Directory of Open Access Journals (Sweden)

    Regina Motta

    2014-06-01

    Full Text Available Pretende-se aqui, analisar o processo de integração nacional no Brasil a partir de 1950 buscando na trajetória do planejamento estatal – nas fases autoritária e pós-redemocratização (neoliberal –, as mudanças de discurso legitimadores das politicas de desenvolvimento, além de entender, como foram sendo construídas a dar legitimidade às políticas de desenvolvimento regional e de como elas foram necessárias em determinado momento e, em outro, “desnecessárias”. Assim, as ideias do regional no contexto brasileiro é trabalhada a partir do contexto socioeconômico ao qual se insere: a atuação de agentes econômicos e a interferência mínima do Estado neoliberal implicaram na concentração de riqueza, seletividade espacial e produção ampliada da pobreza.

  2. South America electric power integration: an utopia or a reality?; Integracao eletrica sul-americana: utopia ou realidade?

    Energy Technology Data Exchange (ETDEWEB)

    Velez, Jaime

    2007-07-01

    The aim of this chapter is the discussing on the economic and institutional conditions necessary to the South America electric power integration in the context of the changes in the electric industry initiated on the 1980's years.

  3. Biochemical and biological characterization of two serine proteinases from Colombian Crotalus durissus cumanensis snake venom.

    Science.gov (United States)

    Patiño, Arley Camilo; Pereañez, Jaime Andrés; Gutiérrez, José María; Rucavado, Alexandra

    2013-03-01

    Two clotting serine proteinases, named Cdc SI and Cdc SII, were isolated and characterized for the first time from Colombian Crotalus durissus cumanensis snake venom. The enzymes were purified using two chromatographic steps: molecular exclusion on Sephacryl S-200 and RP-HPLC on C8 Column. The molecular masses of the proteins, determined by MALDI-TOF mass spectrometry, were 28,561.4 and 28,799.2 Da for Cdc SI and Cdc SII, respectively. The aim of the present study was to evaluate enzymatic, coagulant and toxic properties of the two enzymes. The serine proteinases hydrolyzed specific chromogenic substrate (BaPNA) and exhibited a Michaelis-Menten behavior. Cdc SI had V(max) of 0.038 ± 0.003 nmol/min and K(M) of 0.034 ± 0.017 mM, while Cdc SII displayed values of V(max) of 0.267 ± 0.011 nmol/min and K(M) of 0.145 ± 0.023 mM. N-terminal sequences were VIGGDEXNIN and VIGGDICNINEHNFLVALYE for Cdc SI and Cdc SII, respectively. Molecular masses, N-terminal sequences, inhibition assays, and enzymatic profile suggest that Cdc SI and Cdc SII belong to the family of snake venom thrombin-like enzymes. These serine proteinases differed in their clotting activity on human plasma, showing a minimum coagulant dose of 25 μg and 0.571 μg for Cdc SI and Cdc SII, respectively. Enzymes also showed coagulant activity on bovine fibrinogen and degraded chain α of this protein. Toxins lack hemorrhagic and myotoxic activities, but are capable to induce defibrin(ogen)ation, moderate edema, and an increase in vascular permeability. These serine proteinases may contribute indirectly to the local hemorrhage induced by metalloproteinases, by causing blood clotting disturbances, and might also contribute to cardiovascular alterations characteristic of patients envenomed by C. d. cumanensis in Colombia.

  4. Renal and vascular effects of Crotalus durissus cumanensis venom and its crotoxin fraction

    Directory of Open Access Journals (Sweden)

    TP Pereira

    2011-01-01

    Full Text Available In this study, we evaluated the actions of Crotalus durissus cumanensis venom (CDCmV, and its crotoxin (Crtx fraction, on renal and vascular functions in Wistar rats. In isolated perfused kidneys, CDCmV (10 µg/mL significantly increased the perfusion pressure (PP from 110.7 ± 2.4 to 125.3 ± 2.8 mmHg after 30 minutes. This effect was accompanied by an increased renal vascular resistance (RVR from 5.4 ± 0.1 to 6.2 ± 0.2 mmHg/mL.g-1.min-1. We observed decreases in urinary flow (UF from 0.13 ± 0.01 to 0.05 ± 001 mL.g-1.min-1 and glomerular filtration rate (GFR from 0.66 ± 0.06 to 0.18 ± 0.02 mL.g-1.min-1. Crtx did not change PP or RVR, but diminished GFR (from 0.65 ± 0.05 to 0.26 ± 003 mL.g-1.min-1 and UF (from 0.11 ± 0.008 to 0.09 ± 0.008 mL.g-1.min-1. Both CDCmV and Crtx reduced the percentage of tubular transport of sodium, chloride and potassium. The cytotoxicity of these substances against MDCK cells was tested by the MTT method: only CDCmV caused a decrease in the cell viability with an IC50 of 5.4 µg/mL. In endothelium-intact isolated aortic rings, CDCmV (0.1 to 30 µg/mL increased the sustained phenylephrine-induced contraction to a value of 130.0 ± 6.6% of its corresponding control, but showed a relaxant effect in endothelium-denuded preparations. Similar results were observed in aortic rings contracted with potassium (40 mM. Crtx was ineffective in aortic ring assays. Thus, it is reasonable to suggest that the renal effects induced by the CDCmV may be due to its influence on the endothelium's ability to release factors that can alter the contractile behavior of vascular smooth muscle. In conclusion, CDCmV is toxic to kidney cells. It changes parameters of the renal function including the glomerular filtration rate, renal vascular resistance and tubular transport. The actions induced by CDCmV also involve endothelium-dependent vasoactive properties. Their effects may be only partially attributed to Crtx.

  5. Ontogenetic shift in response to prey-derived chemical cues in prairie rattlesnakes Crotalus viridis viridis

    Institute of Scientific and Technical Information of China (English)

    Anthony J.SAVIOLA; David CHISZAR; Stephen P.MACKESSY

    2012-01-01

    Snakes often have specialized diets that undergo a shift from one prey type to another depending on the life stage of the snake.Crotalus viridis viridis (prairie rattlesnake) takes different prey at different life stages,and neonates typically prey on ectotherms,while adults feed almost entirely on small endotherms.We hypothesized that elevated rates of tongue flicking to chemical stimuli should correlate with particular prey consumed,and that this response shifts from one prey type to another as individuals age.To examine if an ontogenetic shift in response to chemical cues occurred,we recorded the rate of tongue flicking for 25 neonate,20 subadult,and 20 adult (average SVL=280.9,552,789.5 mm,respectively) wild-caught C.v.viridis to chemical stimuli presented on a cotton-tipped applicator; water-soluble cues from two ectotherms (prairie lizard,Sceloporus undulatus,and house gecko,Hemidactylusfrenatus),two endotherms (deer mouse,Peromyscus maniculatus and lab mouse,Mus musculus),and water controls were used.Neonates tongue flicked significantly more to chemical cues of their common prey,S.undulatus,than to all other chemical cues; however,the response to this lizard's chemical cues decreased in adult rattlesnakes.Subadults tongue flicked with a higher rate of tongue flicking to both S.undulatus and P.maniculatus than to all other treatments,and adults tongue flicked significantly more to P.maniculatus than to all other chemical cues.In addition,all three sub-classes demonstrated a greater response for natural prey chemical cues over chemical stimuli of prey not encountered in the wild (M.musculus and H.frenatus).This shift in chemosensory response correlated with the previously described ontogenetic shifts in C.v.viridis diet.Because many vipers show a similar ontogenetic shift in diet and venom composition,we suggest that this shift in prey cue discrimination is likely a general phenomenon among viperid snakes.

  6. Clinical and hematological alterations in dogs during experimental envenomation with Crotalus durissus terrificus venom and treated with antiophidic serum

    Directory of Open Access Journals (Sweden)

    R. M. B. Nogueira

    2006-04-01

    Full Text Available The present work aimed to evaluate the clinical and hematological aspects during experimental envenomation by Crotalus durissus terrificus in dogs treated with different antiophidic serum doses. Sixteen dogs were divided into two groups of eight animals each. Group I received 1mg/kg venom subcutaneously and 30mg antiophidic serum intravenously; Group II received 1mg/kg venom subcutaneously and 60mg antiophidic serum intravenously. In the clinical evaluation, we observed ataxia, moderate sedation, dilated pupils, sialorrhea, flaccid paralysis of mandibular muscles, and discreet edema at the site of venom inoculation. Evaluating red and white blood cells, we observed a decrease of hemoglobins, globular volume and erythrocytes, and an increase of plasmatic proteins, leukocytes, neutrophils, monocytes and lymphocytes. Clotting time increased and there was blood incoagulability with return to normal clotting time six hours after antiophidic serum administration. Animals treated with six antiophidic serum flasks had a faster recovery than the animals that received three serum flasks.

  7. Preparation of crotalus venom radiolabeled with technetium{sup 99m} as a tool for biodistribution study

    Energy Technology Data Exchange (ETDEWEB)

    Pujatti, Priscilla Brunelli; Santos, Raquel Gouvea dos [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN), Belo Horizonte, MG (Brazil)]. E-mail: santosr@cdtn.br; Simal, Carlos Jorge Rodrigues [Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil). Faculdade de Medicina

    2005-10-15

    Technetium-{sup 99m} ({sup 99m} Tc) has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, {sup 99m} Tc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV) has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with {sup 99m} Tc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological activity was preserved. The results suggest that {sup 99m} Tc-CV can be a useful tool for biodistribution studies. (author)

  8. Additional observations and notes on the natural history of the prairie rattlesnake (Crotalus viridis) in Colorado.

    Science.gov (United States)

    Fitzgerald, Kevin T; Shipley, Bryon K; Newquist, Kristin L; Vera, Rebecca; Flood, Aryn A

    2013-11-01

    On account of their unique anatomy, physiology, natural history, ecology, and behavior, rattlesnakes make ideal subjects for a variety of different scientific disciplines. The prairie rattlesnake (Crotalus viridis) in Colorado was selected for investigation of its relationship to colonies of black-tailed prairie dogs (Cynomys ludovicianus) with regard to spatial ecology. A total of 31 snakes were anesthetized and had radiotransmitters surgically implanted. In addition, at the time of their capture, all snakes underwent the following: (1) they had bacterial culture taken from their mouths for potential isolation of pathogenic bacteria; (2) similarly, they had cloacal bacterial cultures taken to assess potentially harmful bacteria passed in the feces; and (3) they had blood samples drawn to investigate the presence of any zoonotic agents in the serum of the snakes. The results of the study and their implications are discussed here. Traditionally, a low incidence of bacterial wound infection has been reported following snakebite. Nevertheless, the oral cavity of snakes has long been known to house a wide variety of bacterial flora. In our study, 10 different bacterial species were isolated from the mouths of the rattlesnakes, 6 of which are capable of being zoonotic pathogens and inducing human disease. More studies are necessary to see why more rattlesnake bites do not become infected despite the presence of such pathogenic bacteria. The results of fecal bacteria isolated revealed 13 bacterial species, 12 of which can cause disease in humans. Of the snakes whose samples were cultured, 26% were positive for the presence of the pathogen Salmonella arizonae, one of the causative agents of reptile-related salmonellosis in humans. It has long been reported that captive reptiles have a much higher incidence than wild, free-ranging species. This study shows the incidence of Salmonella in a wild, free-ranging population of rattlesnakes. In addition, Stenotrophomonas

  9. Eficiência de um conjunto fotovoltaico em condições reais de trabalho na região de Cascavel - DOI: 10.4025/actascitechnol.v30i2.5496

    Directory of Open Access Journals (Sweden)

    Estor Gnoatto

    2008-10-01

    Full Text Available No presente trabalho, foi desenvolvido um estudo sobre um sistema fotovoltaico autônomo, com armazenamento de energia, instalado na Estação Experimental Agrometeorológica da Universidade Estadual do Oeste do Paraná, Campus Cascavel, a uma Latitude 24°59' Sul, Longitude de 53°26' Oeste, com altitude de 682 m, em condições reais de campo, durante o ano de 2002. Foi avaliada a eficiência dos painéis fotovoltaicos em condições reais de campo, sua curva característica foi determinada utilizando, na aquisição de dados, um micrologger da Campbell Scientific-Inc, modelo CR10X, programado para realizar uma leitura por segundo de cada canal e armazenar a média aritmética de 5 minutos dos dados das componentes de radiação solar global no plano horizontal e global na incidência do painel; temperatura ambiente e temperatura de operação do painel; tensão e corrente do sistema fotovoltaico. Os resultados mostram que a eficiência média mensal registrou valores na faixa de 8,65 a 9,17%, com uma média de 8,84%, com variação percentual entre o índice máximo e mínimo de 5,67%. Pode, assim, representar fornecimento médio mensal de energia de 5,57 kWh por painel.

  10. Distribución y representatividad de las especies del género Crotalus en las áreas naturales protegidas de México Distribution and representation of Crotalus species within the natural protected areas of Mexico

    Directory of Open Access Journals (Sweden)

    Dinorah Malinaly Paredes-García

    2011-06-01

    Full Text Available Se realizó un análisis sistemático de la distribución de las especies del género Crotalus en México para conocer los sitios de mayor concentración y evaluar su nivel de representatividad dentro del esquema federal de áreas naturales protegidas (ANP. A partir de registros georreferenciados se construyeron los modelos de distribución potencial de las especies con un número suficiente de registros, empleando los algoritmos GARP y MaxEnt. Con estos mapas de distribución se dedujeron las áreas de mayor concentración de especies y se estimó su presencia en las ANP. La mayor riqueza de especies se concentra en las regiones de la sierra Madre Occidental, el noroeste del Altiplano Mexicano, la porción norte de la sierra Madre Oriental y el extremo oeste de la Faja Volcánica Transmexicana. Se estima que el 84.4% de las ANP incluyen al menos 1 especie de Crotalus y que el 88.2% de las especies que se distribuyen en México están presentes al menos dentro de 1 ANP. En este contexto de alta representatividad, el plantear la incorporación de ANP adicionales para la protección y conservación de crotalinos se dificulta, por lo que debe optarse por estrategias alternativas, tales como educación ambiental, conservación de hábitats mediante el uso sustentable de recursos, y el manejo y reproducción en cautiverio de especies en riesgo; además, se debe fomentar la investigación y el monitoreo de poblaciones.We performed a systematic analysis of the distribution of the Crotalus species in Mexico to depict the places of high species concentration and to evaluate its representation within the federal natural protected areas (NPAs scheme. Based on geo-referenced records, we built distribution models for species with enough records using the GARP and MaxEnt algorithms. We then deduced areas of high species concentration and estimated its presence in the NPAs. The regions with the highest species richness are the Sierra Madre Occidental, the

  11. Comparison of wildlife and captivity rattlesnakes (Crotalus durissus terrificus microbiota Comparação da microbiota de cascavéis (Crotalus durissus terrificus de vida-livre e cativeiro

    Directory of Open Access Journals (Sweden)

    Rui S. Ferreira Junior

    2009-12-01

    Full Text Available The study evaluated and compared the aerobic microbiota from the oral cavity, cloaca and venom of Crotalus durissus terrificus snakes, recently caught from the wild and kept under quarantine (WQ, individual captivity (IC and collective captivity (CC. Antimicrobial drug effectiveness on isolated agents also was assayed. From group I, II and III were isolated, respectively, 29 (63.04%, 38 (90.48% and 21 (42.86% microorganisms from the cloaca; 15 (32.61%, 3 (7.14% and 25 (51.02% microorganisms from the oral cavity; and, 2 (4.35%, 1 (2.38% and 3 (6.12% microorganisms from venom. The most frequent bacteria were Pseudomonas aeruginosa, Proteus vulgaris and Morganella morganii, with sensitivity to amikacin, gentamicin, norfloxacin, sulfazotrin and tobramycin. Snakes kept in semi-open captivity exhibited the fewest microorganisms in oral cavities, perhaps due to the environment in captivity, with different temperature gradients, running water, absence of daily handling, circulating air, possibility of moving around, daily cleaning, and sunlight access.Este estudo avaliou e comparou a microflora aeróbica da cavidade oral, cloaca e veneno de serpentes Crotalus durissus terrificus recém-capturadas da natureza e mantidas sob quarentena (WQ, mantidas em cativeiro coletivo (CC e em cativeiro individual (IC. A eficácia de drogas antimicrobianas de agentes isolados foi também avaliada. Foram isolados microorganismos dos grupos I, II e III respectivamente: 29 (63.04%, 38 (90.48% e 21 (42.86% da cloaca; 15 (32.61%, 3 (7.14% e 25 (51.02% da cavidade oral, e finalmente 2 (4.35%, 1 (2.38% e 3 (6.12% do veneno. As bactérias mais frequentes foram Pseudomonas aeruginosa, Proteus vulgaris e Morganella morganii, com sensibilidade para amikacina, gentamicina, norfloxacina, sulfazotrina e tobramicina. Serpentes mantidas no cativeiro semi-aberto mostraram menor número de agentes infecciosos em cavidade oral, talvez devido ao ambiente de cativeiro com diferentes

  12. An application of randomization for detecting evidence of thermoregulation in timber rattlesnakes (Crotalus horridus) from northwest Arkansas.

    Science.gov (United States)

    Wills, C A; Beaupre, S J

    2000-01-01

    Most reptiles maintain their body temperatures within normal functional ranges through behavioral thermoregulation. Under some circumstances, thermoregulation may be a time-consuming activity, and thermoregulatory needs may impose significant constraints on the activities of ectotherms. A necessary (but not sufficient) condition for demonstrating thermoregulation is a difference between observed body temperature distributions and available operative temperature distributions. We examined operative and body temperature distributions of the timber rattlesnake (Crotalus horridus) for evidence of thermoregulation. Specifically, we compared the distribution of available operative temperatures in the environment to snake body temperatures during August and September. Operative temperatures were measured using 48 physical models that were randomly deployed in the environment and connected to a Campbell CR-21X data logger. Body temperatures (n=1,803) were recorded from 12 radiotagged snakes using temperature-sensitive telemetry. Separate randomization tests were conducted for each hour of day within each month. Actual body temperature distributions differed significantly from operative temperature distributions at most time points considered. Thus, C. horridus exhibits a necessary (but not sufficient) condition for demonstrating thermoregulation. However, unlike some desert ectotherms, we found no compelling evidence for thermal constraints on surface activity. Randomization may prove to be a powerful technique for drawing inferences about thermoregulation without reliance on studies of laboratory thermal preference.

  13. Single-step purification of crotapotin and crotactine from Crotalus durissus terrificus venom using preparative isoelectric focusing

    Directory of Open Access Journals (Sweden)

    Aguiar A.S.

    1997-01-01

    Full Text Available We describe the isolation of crotoxin, a presynaptic B-neurotoxin, as well as its subunits B (crotactine and A (crotapotin from lyophilized Crotalus durissus terrificus venom by a single-step preparative isoelectric focusing procedure. From 98 mg of dried venom protein 20.1 mg of crotactine and 13.1 mg of crotapotin were recovered in the first step of focalization and 4.2 mg in a second run. These values correspond to 35.7% of the total venom protein applied. Crotactine separated in the 9.3-7.0 pH range (tubes 1-6 and crotapotin in the 1.8-2.8 pH range (tubes 15-19 and both were homogeneous by SDS-PAGE and N-terminal amino acid analysis. Crotactine, a 12-kDa protein, presented hemolytic and phospholipase A2 activity. Thus, using isoelectric focusing we simultaneously purified both toxins in high yields. This method can be used as an alternative for the purification and characterization of proteins from other snake venoms under conditions in which biological activity is retained

  14. Identification of Lys49-PLA2 from crude venom of Crotalus atrox as a human neutrophil-calcium modulating protein.

    Science.gov (United States)

    Sultan, Md Tipu; Li, Hong-Mei; Lee, Yong Zu; Lim, Soon Sung; Song, Dong-Keun

    2016-03-01

    We fortuitously observed a human neutrophil intracellular free-calcium concentration ([Ca(2+)]i) increasing activity in the commercially available phosphodiesterase I (PDE I), which is actually dried crude venom of Crotalus atrox. As this activity was not observed with another commercially available pure PDE I, we tried to find out the causative molecule(s) present in 'crude' PDE, and identified Lys49-phospholipase A2 (Lys49-PLA2 or K49-PLA2), a catalytically inactive protein which belongs to the phospholipase A2 family, by activity-driven three HPLC (reverse phase, size exclusion, reverse phase) steps followed by SDS-PAGE and LC-MS/MS. K49-PLA2 induced Ca(2+) infl ux in human neutrophils without any cytotoxic eff ect. Two calcium channel inhibitors, 2-aminoetoxydiphenyl borate (2-APB) (30 µM) and SKF-96365 (20 µM) signifi cantly inhibited K49-PLA2-induced [Ca(2+)]i increase. These results suggest that K49-PLA2 modulates [Ca(2+)]i in human neutrophils via 2-APB- and SKF-96365-sensitive calcium channels without causing membrane disruption.

  15. Crotoxin, the major toxin from the rattlesnake Crotalus durissus terrificus, inhibits ³H-choline uptake in guinea pig ileum

    Directory of Open Access Journals (Sweden)

    L.S. Kattah

    2000-09-01

    Full Text Available We examined the effect of crotoxin, the neurotoxic complex from the venom of the South American rattlesnake Crotalus durissus terrificus, on the uptake of ³H-choline in minces of smooth muscle myenteric plexus from guinea pig ileum. In the concentration range used (0.03-1 µM and up to 10 min of treatment, crotoxin decreased ³H-choline uptake by 50-75% compared to control. This inhibition was time dependent and did not seem to be associated with the disruption of the neuronal membrane, because at least for the first 20 min of tissue exposure to the toxin (up to 1 µM the levels of lactate dehydrogenase (LDH released into the supernatant were similar to those of controls. Higher concentrations of crotoxin or more extensive incubation times with this toxin resulted in elevation of LDH activity detected in the assay supernatant. The inhibitory effect of crotoxin on ³H-choline uptake seems to be associated with its phospholipase activity since the equimolar substitution of Sr2+ for Ca2+ in the incubation medium or the modification of the toxin with p-bromophenacyl bromide substantially decreased this effect. Our results show that crotoxin inhibits ³H-choline uptake with high affinity (EC25 = 10 ± 5 nM. We suggest that this inhibition could explain, at least in part, the blocking effect of crotoxin on neurotransmission.

  16. Dosimetria interna de radiofármacos derivados de polipeptídeos antitumorais isolados dos venenos de: Crotalus durissus terrificus e Scorpaena plumieri

    OpenAIRE

    Henrique Martins de Andrade

    2009-01-01

    A identificação de novos agentes terapêuticos e diagnósticos capazes de inibir o crescimento tumoral é essencial para melhoria no prognóstico de pacientes acometidos por tumores malignos (glioma, mama dentre outros). Nesse contexto, produtos naturais (vegetais e animais) constituem-se numa rica fonte de substâncias com potencial antitumoral. Polipeptídeos isolados dos venenos da serpente Crotalus durissus terrificus (Crtx) e do peixe Scorpaena plumieri (SPGP), apresentam atividade...

  17. Influência da temperatura corporal de cascavéis (Crotalus durissus submetidas à anestesia com cetamina Influence of body temperature on rattlesnakes (Crotalus durissus anesthetized with ketamine

    Directory of Open Access Journals (Sweden)

    Adriano B. Carregaro

    2009-12-01

    Full Text Available O estudo objetivou verificar a influência da temperatura corporal nos parâmetros fisiológicos e nos períodos de indução e recuperação anestésicos de cascavéis (Crotalus durissus anestesiadas com cetamina. Os animais foram previamente submetidos à hipotermia (HIPO (The aim of the study was to verify the influence of the body temperature under physiological values and latency and recovery times on rattlesnakes anesthetized with ketamine. The animals were previously submitted to hypothermia (HYPO (<22°C and normothermia (30°C (NORMO and then, anesthetized with 80 mg/kg IM of ketamine. Latency and recovery times were evaluated by head tonus, muscular tonus and righting reflex. Heart rate (HR, time of apnea and body temperature were measured before and 5, 10, 15, 30, 60, 90 and 120 minutes after ketamine administration. Blood gases parameters were measured before, 30 and 60 minutes. It was not observed difference on latency time in both groups. The recovery time was higher on HYPO (5,5 hours compared to NORMO (3,5 hours. HR was higher on NORMO compared to HYPO. Time of apnea was the same pattern on both groups. Compared to basal levels, time of apnea was shorter between 5 to 30 min on both groups. Respiratory acidosis was observed only at 0 min in NORMO. SvO2 was higher after 30 min, the same as with PvO2 in both groups. PvCO2 reduced after 30 min in both groups. It was evident that body temperature exerts intense influence on the recovery time on rattlesnakes anesthetized with ketamine.

  18. EFECTO DEL AZUL DE METILENO EN LA HIPOTENSIÓN Y BRADICARDIA ASOCIADAS A LA ADMINISTRACIÓN DE UNA DOSIS LETAL DEL VENENO TOTAL DE CASCABEL VENEZOLANA (Crotalus durissus cumanensis) EN RATAS

    DEFF Research Database (Denmark)

    Briceno, Elena; Zerpa, Hector; Ascanio, Elias

    2010-01-01

    The effects of pre treatment with methylene blue (MB) on the cardiovascular effect caused by the administration of total venom (TV) of Crotalus durissus cumanensis was studied in adult rats, allocated into two groups: group I, six rats treated with TV (single dose: 1.5 mg/kg, IV) and group II, si...

  19. Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768

    Directory of Open Access Journals (Sweden)

    D. P. Netto

    2004-01-01

    Full Text Available Natural (NV and Cobalto60-irradiated (IrV Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0 and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV and the other natural venom (NV. Three antigen doses (venom were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC, total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD50 for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/ml indicates that slight leukocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the NV group, showing its greater toxicity.

  20. Mitochondrial dysfunction induced by pancreatic and crotalic (Crotalus durissus terrificus) phospholipases A2 on rabbit proximal tubules suspensions.

    Science.gov (United States)

    Amora, Daniela N; Costa Martins, Alice M; Roeser, Nancy; Senter, Ruth; Ostrowsky, Tiffany; Weinberg, Joel M; Monteiro, Helena S A

    2008-12-15

    In the present study we show that phospholipases A2 isolated from porcine pancreas (PP-PLA2) and Crotalus durissus terrificus snake venom (SV-PLA2) induced dose-dependent increases of LDH release from rabbit proximal tubules in suspension. Both porcine and crotalic PLA(2)s induced increases in non-esterified fatty acid (NEFA) levels (microg of NEFA/mg of tubule protein). It was observed that the NEFA levels in the pellets were higher than in the supernatant for both PLA2, and were dose-dependent for the crotalic PLA2 group. Furthermore, snake venom PLA2 induced a decrease in mitochondrial membrane potential (DeltaPsi(m)) assessed by both JC-1 uptake and safranin O uptake. Porcine PLA2 produced no effects on JC-1 uptake with the highest concentrations and an unexpected increase in the group treated with the lowest concentration. In contrast, the safranin O method revealed decreases of energization with both phospholipases, so it had higher sensitivity to the presence of the increased NEFA levels. Addition of delipidated bovine serum albumin (dBSA) completely reversed the effects induced by phospholipases on DeltaPsi(m) measured with safranin O. Incubation with pancreatic and crotalic phospholipases A2 produced no changes on cell ATP levels. We conclude that the treatment of proximal tubule suspensions with porcine or crotalic phospholipases disturbed membrane integrity as well as mitochondrial function. Specific early NEFA-mediated mitochondrial effects of the phospholipases used in the present study are indicated by the benefit provided by dBSA.

  1. Molecular models of the Mojave rattlesnake (Crotalus scutulatus scutulatus) venom metalloproteinases reveal a structural basis for differences in hemorrhagic activities.

    Science.gov (United States)

    Dagda, Ruben K; Gasanov, Sardar E; Zhang, Boris; Welch, William; Rael, Eppie D

    2014-03-01

    Rattlesnake venom can differ in composition and in metalloproteinase-associated activities. The molecular basis for this intra-species variation in Crotalus scutulatus scutulatus (Mojave rattlesnake) remains an enigma. To understand the molecular basis for intra-species variation of metalloproteinase-associated activities, we modeled the three-dimensional structures of four metalloproteinases based on the amino acid sequence of four variations of the proteinase domain of the C. s. scutulatus metalloproteinase gene (GP1, GP2, GP3, and GP4). For comparative purposes, we modeled the atrolysin metalloproteinases of C. atrox as well. All molecular models shared the same topology. While the atrolysin metalloproteinase molecular models contained highly conserved substrate binding sites, the Mojave rattlesnake metalloproteinases showed higher structural divergence when superimposed onto each other. The highest structural divergence among the four C. s. scutulatus molecular models was located at the northern cleft wall and the S'1-pocket of the substrate binding site, molecular regions that modulate substrate selectivity. Molecular dynamics and field potential maps for each C. s. scutulatus metalloproteinase model demonstrated that the non-hemorrhagic metalloproteinases (GP2 and GP3) contain highly basic molecular and field potential surfaces while the hemorrhagic metalloproteinases GP1 and atrolysin C showed extensive acidic field potential maps and shallow but less dynamic active site pockets. Hence, differences in the spatial arrangement of the northern cleft wall, the S'1-pocket, and the physico-chemical environment surrounding the catalytic site contribute to differences in metalloproteinase activities in the Mojave rattlesnake. Our results provide a structural basis for variation of metalloproteinase-associated activities in the rattlesnake venom of the Mojave rattlesnake.

  2. Experimentally altered navigational demands induce changes in the cortical forebrain of free-ranging northern pacific rattlesnakes (Crotalus o. oreganus).

    Science.gov (United States)

    Holding, Matthew L; Frazier, Julius A; Taylor, Emily N; Strand, Christine R

    2012-01-01

    The hippocampus of birds and mammals plays a crucial role in spatial memory and navigation. The hippocampus exhibits plasticity in adulthood in response to diverse environmental factors associated with spatial demands placed on an animal. The medial and dorsal cortices of the telencephalon of squamate reptiles have been implicated as functional homologues to the hippocampus. This study sought to experimentally manipulate the navigational demands placed on free-ranging northern Pacific rattlesnakes (Crotalus o. oreganus) to provide direct evidence of the relationship between spatial demands and neuroplasticity in the cortical telencephalon of the squamate brain. Adult male rattlesnakes were radio-tracked for 2 months, during which time 1 of 3 treatments was imposed weekly, namely 225-meter translocation in a random direction, 225-meter walk and release at that day's capture site (handling control) or undisturbed (control). Snakes were then sacrificed and the brains were removed and processed for histological analysis of cortical features. The activity range was larger in the translocated (Tr) group compared to the handled (Hd) and undisturbed control (Cn) groups when measured via 95% minimum convex polygon (MCP). At the 100% MCP level, Tr snakes had larger activity ranges than the Cn snakes only. The volume of the medial cortex (MC) was larger in the Tr group compared to the Cn group. The MC of Hd snakes was not significantly different from that of either of the other groups. No differences in dorsal cortex (DC) or lateral cortex volumes were detected among the groups. Numbers of 5-bromo-2'-deoxyuridine (BrdU)-labeled cells in the MC and DC 3 weeks after BrdU injection were not affected by treatment. This study establishes a causal relationship between navigational demands and greater MC volume in a free-ranging reptile.

  3. Impactos ambientais decorrentes da ocupação de áreas reguladoras do aporte de areia: a planície Costeira da Caponga, município de Cascavel, litoral leste cearense.

    Directory of Open Access Journals (Sweden)

    Antonio Jeovah de Andrade Meireles

    2008-03-01

    Full Text Available Pour définir les impacts environnementaux associés à l'occupation humaine dans des secteurs caractérisés par l'apport de sédiments (bypass sur la plage, via des champ de dunes et l’estuaire, ont été réalisées des études géo-environnementales dans la plaine côtière du Caponga, en y incluant un promontoire, une bande littorale et l’embouchure du canal estuarien de même nom. Les résultats ont été représentés dans des modèles évolutifs, intégrés à partir de la définition des flux de matière et d'énergie qui transitent dans la zone côtière. Les conséquences de l'expansion urbaine sur le promontoire, les dunes, la plage et le canal estuarien ont été caractérisées afin de définir des mesures adaptées de planification et de gestion.Environmental impacts caused by the occupation of areas regulating the sand input to the coast line in the coastal plain of Caponga, Cascavel town, East Coast of Ceará. To determine the environmental impacts related to human occupation in areas characterized by the input of sediments (bypass to the coastal line, through dunes and estuary, we carried out geoenvironmental studies in the coastal plain of Caponga. They included sections represented by a headland, coastal line and river mouth of the estuarine channel of Caponga. The results were represented in integrated evolutionary models based on the definition of flows of matter and energy that occur in the coastal area. The effects of urban expansion on the headland, dunes, shore and estuarine channel were characterized so as to define adequate measures of planning and management.Para definir os impactos ambientais associados à ocupação humana em áreas caracterizadas pelo aporte de sedimentos (bypass para a faixa de praia, via campo de dunas e estuário, foram realizados estudos geoambientais na planície costeira da Caponga, abrangendo setores representados por um promontório, faixa litorânea e desembocadura do canal estuarino

  4. Hematological changes in sheep inoculated with natural and Cobalt{sub 60}-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

    Energy Technology Data Exchange (ETDEWEB)

    Netto, D.P.; Alfieri, A.A.; Balarim, M.R.S. [Universidade Estadual de Londrina, PR (Brazil). Faculdade de Medicina Veterinaria e Zootecnia. Dept. de Medicina Veterinaria Preventiva]. E-mail: rnetto@uel.br; Chiacchio, S.B.; Bicudo, P.L. [UNESP, Botucatu, SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia; Nascimento, N. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Supervisao de Radiobiologia

    2004-07-01

    Natural (NV) and Cobalt{sub 60}-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 m M, p H 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count, total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD{sub 50} for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/{mu}l indicates that slight leucocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the N V group, showing its greater toxicity. (author)

  5. Crotoxin from Crotalus durissus terrificus is able to down-modulate the acute intestinal inflammation in mice.

    Directory of Open Access Journals (Sweden)

    Caroline de Souza Almeida

    Full Text Available Inflammatory bowel diseases (IBD is the result of dysregulation of mucosal innate and adaptive immune responses. Factors such as genetic, microbial and environmental are involved in the development of these disorders. Accordingly, animal models that mimic human diseases are tools for the understanding the immunological processes of the IBD as well as to evaluate new therapeutic strategies. Crotoxin (CTX is the main component of Crotalus durissus terrificus snake venom and has an immunomodulatory effect. Thus, we aimed to evaluate the modulatory effect of CTX in a murine model of colitis induced by 2,4,6- trinitrobenzene sulfonic acid (TNBS. The CTX was administered intraperitoneally 18 hours after the TNBS intrarectal instillation in BALB/c mice. The CTX administration resulted in decreased weight loss, disease activity index (DAI, macroscopic tissue damage, histopathological score and myeloperoxidase (MPO activity analyzed after 4 days of acute TNBS colitis. Furthermore, the levels of TNF-α, IL-1β and IL-6 were lower in colon tissue homogenates of TNBS-mice that received the CTX when compared with untreated TNBS mice. The analysis of distinct cell populations obtained from the intestinal lamina propria showed that CTX reduced the number of group 3 innate lymphoid cells (ILC3 and Th17 population; CTX decreased IL-17 secretion but did not alter the frequency of CD4+Tbet+ T cells induced by TNBS instillation in mice. In contrast, increased CD4+FoxP3+ cell population as well as secretion of TGF-β, prostaglandin E2 (PGE2 and lipoxin A4 (LXA4 was observed in TNBS-colitis mice treated with CTX compared with untreated TNBS-colitis mice. In conclusion, the CTX is able to modulate the intestinal acute inflammatory response induced by TNBS, resulting in the improvement of clinical status of the mice. This effect of CTX is complex and involves the suppression of the pro-inflammatory environment elicited by intrarectal instillation of TNBS due to the

  6. Avaliação da resposta inflamatória hematológica em cascavéis (Crotalus durissus Linnaeus, 1758 inoculadas com BCG Assessment of blood inflammatory response in BCG stimulated rattlesnakes (Crotalus durissus Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Wellington Bandeira da Silva

    2009-12-01

    Full Text Available A criação de serpentes peçonhentas em cativeiro para produção de soros antipeçonhas possui crescente importância para a saúde pública devido ao aumento do número de notificações de acidentes ofídicos a cada ano no Brasil. Iniciado no século XX, ainda hoje essa atividade apresenta alguns desafios como a instalação de doenças no plantel. O hemograma é um exame de triagem clínica que auxilia no diagnóstico de diversas moléstias que acometem diferentes espécies de animais, no entanto ainda pouco estudado em serpentes. A caracterização das alterações hematológicas em cascavéis inoculadas experimentalmente com BCG pode servir de base na utilização deste exame no auxílio ao diagnóstico de infecções bacterianas na espécie. Dessa forma, foram realizados exames hematológicos em 10 serpentes da espécie Crotalus durissus pertencentes ao plantel da Divisão de Herpetologia do Instituto Vital Brazil. Os animais foram divididos em dois grupos (Grupos 1 e 2, homogêneos entre si em relação ao peso e proporção sexual. Os dois grupos foram inoculados com BCG e submetidos à coleta de sangue antes da inoculação e em três momentos pós-inoculação (3º, 5º, e 7º dias para o Grupo 1 e 11º, 17º e 21º dias para o Grupo 2. O hemograma foi realizado por método semidireto pela utilização de líquido de Natt e Herrick e as lâminas foram coradas pelo Giemsa. Observou-se anemia discreta, com redução dos valores de concentração de hemoglobina corpuscular média e da hemoglobina globular média no Grupo 1 que foi relacionada à doença inflamatória. A trombocitopenia observada no Grupo 2 sugeriu a atuação deste tipo celular em processos inflamatórios. Um único animal do Grupo 1 apresentou granulocitose e alguns animais apresentaram discreta azurofilia. Observaram-se alterações morfológicas nos leucócitos. Os granulócitos apresentaram granulações grosseiras e os azurófilos apresentaram aumento de tamanho e

  7. Snakebites by Crotalus durissus ssp in children in Campinas, São Paulo, Brazil Acidentes por serpentes Crotalus durisssus ssp em crianças em Campinas, São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Fábio BUCARETCHI

    2002-01-01

    Full Text Available From January, 1984 to March, 1999, 31 children under 15 y old (ages 1-14 y, median 8 y were admitted after being bitten by rattlesnakes (Crotalus durissus ssp. One patient was classified as "dry-bite", 3 as mild envenoming, 9 as moderate envenoming and 18 as severe envenoming. Most patients had neuromuscular manifestations, such as palpebral ptosis (27/31, myalgia (23/31 and weakness (20/31. Laboratory tests suggesting rhabdomyolysis included an increase in total blood creatine kinase (CK, 28/29 and lactate dehydrogenase (LDH, 25/25 levels and myoglobinuria (14/15. The main local signs and symptoms were slight edema (20/31 and erythema (19/31. Before antivenom (AV administration, blood coagulation disorders were observed in 20/25 children that received AV only at our hospital (incoagulable blood in 17/25. AV early reactions were observed in 20 of these 25 cases (9/9 patients not pretreated and 11/16 patients pretreated with hydrocortisone and histamine H1 and H2 antagonists. There were no significant differences in the frequency of patients with AV early reactions between the groups that were and were not pretreated (Fisher's exact test, p = 0.12. Patients admitted less than and more than 6 h after the bite showed the same risk of developing severe envenoming (Fisher's exact test, p = 1. No children of the first group (De janeiro de 1984 a março de 1999, 31 crianças com menos de 15 anos de idade (1 a 14 anos, mediana = 8 anos foram admitidas após terem sido picadas por Crotalus durissus ssp. Uma criança não apresentou manifestações clínicas de envenenamento, enquanto 3 foram classificadas como acidente leve, 9 como moderado e 18 como grave. A maioria das crianças apresentou envolvimento neuromuscular, tais como ptose palpebral (27/31, mialgia (23/31 e fraqueza (20/31. Alterações laboratoriais sugerindo rabdomiólise também foram observadas, como aumento das enzimas séricas CK (28/29 e LDH (25/25 e mioglobinúria (14/15. As

  8. Produção e caracterização parcial de nanocorpos ativos contra Crotoxina: uma neurotoxina da serpente Crotalus durissus terrificus.

    OpenAIRE

    Luiz, Marcos Barros

    2014-01-01

    Dissertação apresentada ao Programa de Pós- Graduação: Mestrado em Biologia Experimental (PGBIOEXP) da Fundação Universidade Federal de Rondônia (UNIR) como requisito final para a obtenção do título de Mestre em Biologia Experimental. Orientadora: Prof. Dra. Carla Freire Celedônio Fernandes. No Brasil cerca de 7,5 % dos casos de envenenamento ofídico são causados por serpentes do gênero Crotalus, os quais originam uma taxa de mortalidade de 1,8 %. Os efeitos neurotóxicos, miotóxicos e nefr...

  9. Genetic diversity and genetic structure of an endemic Mexican Dusky Rattlesnake (Crotalus triseriatus) in a highly modified agricultural landscape: implications for conservation.

    Science.gov (United States)

    Sunny, Armando; Monroy-Vilchis, Octavio; Zarco-González, Martha M; Mendoza-Martínez, Germán David; Martínez-Gómez, Daniel

    2015-12-01

    It is necessary to determine genetic diversity of fragmented populations in highly modified landscapes to understand how populations respond to land-use change. This information will help guide future conservation and management strategies. We conducted a population genetic study on an endemic Mexican Dusky Rattlesnake (Crotalus triseriatus) in a highly modified landscape near the Toluca metropolitan area, in order to provide crucial information for the conservation of this species. There was medium levels of genetic diversity, with a few alleles and genotypes. We identified three genetically differentiated clusters, likely as a result of different habitat cover type. We also found evidence of an ancestral genetic bottleneck and medium values of effective population size. Inbreeding coefficients were low and there was a moderate gene flow. Our results can be used as a basis for future research and C. triseriatus conservation efforts, particularly considering that the Trans-Mexican Volcanic Belt is heavily impacted by destructive land-use practices.

  10. Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake venom treatment

    Directory of Open Access Journals (Sweden)

    B. P. Tamieti

    2007-01-01

    Full Text Available Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis.

  11. Intraspecies differences in hemostatic venom activities of the South American rattlesnakes, Crotalus durissus cumanensis, as revealed by a range of protease inhibitors.

    Science.gov (United States)

    Salazar, Ana M; Aguilar, Irma; Guerrero, Belsy; Girón, María E; Lucena, Sara; Sánchez, Elda E; Rodríguez-Acosta, Alexis

    2008-09-01

    Crotalus durissus cumanensis is an endemic rattlesnake found in Venezuela and Colombia. In this study, a comparative analysis of hemorrhagic, coagulation and fibrino(geno)lytic activities in the presence or absence of protease inhibitors was performed with venoms of the same species Crotalus durissus cumanensis, from seven geographical regions of Venezuela (Lagunetica, Santa Teresa, Carrizales, Guarenas, Anzoátegui, Margarita and Maracay). Lagunetica, Carrizales and Anzoátegui venoms induced hemorrhagic activity. All venoms, except that of snakes from the Carrizales region presented thrombin-like activity, which was inhibited completely by phenylmethanesulfonyl fluoride and ethylene glycol-bis-N, N,N',N'-tetraacetic acid. This effect of the latter could be explained by the high chelant calcium effect, which is a cofactor for the fibrin polymerization process. Soybean trypsin inhibitor was effective on Santa Teresa venom. Antithrombin III/Hep complex and phenantroline partially inhibited this activity in all venoms except Margarita and Anzoátegui, respectively, which were not inhibited. Serine protease inhibitors were more effective against thrombin, kallikrein and plasmin-like amidolytic activities. Additionally, metalloprotease inhibitors significantly inhibited the t-PA-like amidolytic activity and completely the hemorrhagic and fibrino(geno)lytic activities. In conclusion, the thrombin-like activity observed in these venoms was partially reduced by serine protease inhibitors, indicating the possible presence of catalytic domains in those enzymes that do not interact with these inhibitors. Using protease inhibitors on venom hemostatic activities could contribute to our understanding of the active components of snake venom on the hemostatic system, and further reveal the intraspecies variation of venoms, which is important in the treatment of envenomation, and in addition represents an interesting model for the study of venom in hemostasis.

  12. Internal dosimetry of radiopharmaceuticals derived of antitumor polypeptide isolated from venoms: Crotalus durissus terrifucus and Scorpaena plumieri;Dosimetria interna de radiofarmacos derivados de polipeptideos antitumorais isolados dos venenos de: Crotalus durissus terrificus e Scorpaena plumieri

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Henrique Martins de

    2009-07-01

    The identification of new diagnostic and therapeutic agents capable of inhibiting tumor growth is essential for improving the prognosis of patients suffering from malignant tumors (glioma, breast and others). In this context, natural products (plants and animals) are a rich source of substances with potential antitumor. Despite knowledge of the etiology and pathology of tumors little progress has been observed in the area of diagnosis. Molecules of snake venoms have been shown to play an important role not only in the survival and proliferation of tumor cells but also in the process of tumor cell adhesion, migration and angiogenesis. Polypeptides isolated from the venom of the snake, Crotalus durissus terrificus, Crtx, and Scorpaena plumieri fish, SPGP, have antitumor activity against malignant tumors. It was shown that similar radio iodines Crtx and SPGP, {sup 125}I-Crtx and {sup 125}I-SPGP, can interact specifically with malignant tumors and induce cell death. Prototype-based radiopharmaceuticals Crtx and SPGP containing radioiodine 1311 were able to produce diagnostic images to accumulate specifically in the tumor site. The present study aimed at evaluating the potential radiological safety and diagnostic/therapeutic efficacy of {sup 131}I-Crtx {sup l31}I-SPGP and (evaluated from the biokinetic data in mice bearing Ehrlich tumor) were treated by the MIRD formalism to carry out internal dosimetry studies. Absorbed doses due to the uptake of {sup 131}I-Crtx and {sup 131}I-SPGP were determined in various organs of mice and implanted into the tumor. The results obtained for the animal model were extrapolated to humans by assuming a similar concentration ratio among the various tissues between mice and humans. In extrapolation, we used the masses of human organs of the phantom of Cristy/Eckerman. Both radiation penetrating and non penetrating of {sup 131}I on the tissue were considered in dose calculations. The absorbed dose in the bone marrow due to the

  13. Thalidomide and pentoxifylline block the renal effects of supernatants of macrophages activated with Crotalus durissus cascavella venom

    Directory of Open Access Journals (Sweden)

    A.M.C. Martins

    2004-10-01

    Full Text Available Because thalidomide and pentoxifylline inhibit the synthesis and release of tumor necrosis factor-alpha (TNF-alpha, we determined the effect of these drugs on the renal damage induced by supernatants of macrophages activated with Crotalus durissus cascavella venom in order to identify the role of TNF-alpha in the process. Rat peritoneal macrophages were collected with RPMI medium and stimulated in vitro with C.d. cascavella venom (10 µg/ml in the absence and presence of thalidomide (15 µM or pentoxifylline (500 µM for 1 h and washed and kept in culture for 2 h. Supernatant (1 ml was tested on an isolated perfused rat kidney (N = 6 for each group. The first 30 min of each experiment were used as control. The supernatant was added to the perfusion system. All experiments lasted 120 min. The toxic effect of the preparation of venom-stimulated macrophages on renal parameters was determined. At 120 min, thalidomide (Thalid and pentoxifylline (Ptx inhibited (P < 0.05 the increase in perfusion pressure caused by the venom (control = 114.0 ± 1.3; venom = 137.1 ± 1.5; Thalid = 121.0 ± 2.5; Ptx = 121.4 ± 4.0 mmHg, renal vascular resistance (control = 4.5 ± 0.2; venom = 7.3 ± 0.6; Thalid = 4.5 ± 0.9; Ptx = 4.8 ± 0.6 mmHg/ml g-1 min-1, urinary flow (control = 0.23 ± 0.001; venom = 0.44 ± 0.01; Thalid = 0.22 ± 0.007; Ptx = 0.21 ± 0.009 ml g-1 min-1, glomerular filtration rate (control = 0.72 ± 0.06; venom = 1.91 ± 0.11; Thalid = 0.75 ± 0.04; Ptx = 0.77 ± 0.05 ml g-1 min-1 and the decrease in percent tubular sodium transport (control = 77.0 ± 0.9; venom = 73.9 ± 0.66; Thalid = 76.6 ± 1.1; Ptx = 81.8 ± 2.0%, percent tubular chloride transport (control = 77.1 ± 1.2; venom = 71.4 ± 1.1; Thalid = 77.6 ± 1.7; Ptx = 76.8 ± 1.2%, and percent tubular potassium transport (control = 72.7 ± 1.1; venom = 63.0 ± 1.1; Thalid = 72.6 ± 1.0; Ptx = 74.8 ± 1.0%, 30 min before and during the stimulation of macrophages with C.d. cascavella venom

  14. Desafios enfrentados por organizações produtivas populares: um estudo com base em 12 experiências sul-americanas

    OpenAIRE

    Soares, Leonardo de Carvalho; Pinto, Marco Aurélio Cabral

    2013-01-01

    O presente projeto de pesquisa buscou identificar os principais desafios enfrentados por rol selecionado de organizações populares com finalidade lucrativa localizadas na América do Sul durante nove meses ao longo de 2011. Para tanto, realizou-se coleta de dados em 12 organizações distribuídas entre quatro países, em viagem de bicicleta pela América do Sul. Depois da análise dos dados coletados, em que se procurou grupar experiências visitadas em categorias tipo-ideal, concluiu-se que em tod...

  15. The South American energy policies: regional problems and national logics; As politicas energeticas Sul-Americanas: problemas regionais e logicas nacionais

    Energy Technology Data Exchange (ETDEWEB)

    Le Prioux, Bruna [Centro de Pesquisa e Documentacao da America Latina (CREDAL) (France)

    2010-07-01

    The international energy context in the first decade of the 21st century can be described by the following points. First, the growing concerns with climatic changes and the greenhouse effect, which the main cause is the massive use of fossil fuels. Second, the energy vulnerability, due to the mistrust in the main hydrocarbon producers, to the increasing consumption from the so-called developing countries and to the idea of a possible end of oil reserves. And third, as a consequence of the least factor, an intense speculation in international market has increased the prices of oil barrel and gas since 2005. In this context, each country tries to adapt to their way of such changes. Beyond local solutions, South American countries have historic attempts of regional integration through energy, which can be presented as a complement of national policies. This research focuses on the study of the gas energy policy of some producers' and consumers' countries in South America, their choices and procedures in the national and international scope. Thus, the main goal of this article is to analyze how national energy policies affect the regional energy action of these South American countries. In order to answer this question, our goals are: (1) diagnosing the energy potentialities and disadvantages of each country; (2) identifying concepts related to energy questions; and (3) relating the two past steps to analyze the energy interaction in South America. The countries selected to this research are: Brazil, Argentina and Chile, due to their economic magnitude in South America and their intense energy consumption; and Bolivia and Venezuela, due to their energy reserves and surplus. The study of national energy systems was made through SWOT analysis (Strengths, Weaknesses, Opportunities and Threat), in order to have a synthetically diagnosis about the energetic potentials and disadvantages of each country. Thereafter, we intersect this data with concepts as sustainable development, interdependence and energy governance, to relate the national and international energy policies. Energy national policies are relevant factors to be considered when a regional integration is studied. In fact, energy advantages and deficiencies emerge in regional scenario, showing that the interdependences depend on national logics and strategies. In this sense, we consider that the effect of energy integration in South America is unsatisfying because national policies do not converge. The advantages of integration (i.e. commerce increase, ensuring supplies and reducing price volatility) are neutralized without this countries' coordination. (author)

  16. Crustal evolution of South American Platform based on Sm-Nd isotope geochemistry; Evolucao crustal da plataforma sul americana com base na geoquimica isotopica Sm-Nd

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Kei

    1998-07-01

    Sm-Nd isotopic systematics is relevant to the topics of origin and evolution the of continental crust, where model ages refer to the time when crustal material was differentiated from the upper mantle. Alternative interpretations are due to a lack of adequate information on crustal processes and the variable composition of the mantle sources. The Sm-Nd methods are presented, and applied on rock materials from the South American Platform. The main conclusions indicate juvenile accretion with higher growth rates (peaks), around 3.7-3.5 Ga ({approx} 0.5% in volume), 3.1 - 2.9 Ga ({approx}16%), 2.7 - 2.6 ({approx} 9%), 2.2 - 1.9 (35%) and 1.3-1.0 (7%). The continental growth curve indicates that about 35 % of the crust was formed by 2.5 Ga, 88% by 1.8 Ga and 99% by 1.0 Ga, and the remaining {approx} 1 % was added in the Phanerozoic. Rapid crustal growth occurred between 2.2 and 1.9 Ga. The main period of continental crust formation occurred during the Paleoproterozoic, corresponding to 54 % in volume. Sm-Nd model ages, when compared with the crystallisation ages of granitoid rocks, furnish a rough estimate of juvenile vs. reworked material. Within the South American Platform about 45% of juvenile continental crust is still preserved within tectonic provinces of different ages. The remainder represents continental crust reworked in younger tectono-thermal events. In particular crustal reworking was predominating over juvenile accretion during Meso-Neoproterozoic. The Transbrasiliano Lineament is a megasuture, active in the Neoproterozoic, which separates a large northwestern mass, including the Amazonian and Sao Luis Cratons, from a southeastern mass, formed by a collage of cratonic fragments, of which the Sao Francisco and Rio de La Plata are the largest. The crustal evolutions of these two large continental masses are considered individually, and can be resumed following form: I - Old Archean rocks (>3.4 Ga) are found only within the south-eastern part (Gaviao Block, Contendas-Mirante Complex (Sao Francisco Craton) and Caldas Brandao Massif (Borborema Province); 2 - On both continental masses, crustal evolution between 3.0 and 1.7 Ga is very similar; 3 - During Meso and Neoproterozoic times, the northwestern mass (Amazonian and Sao Luis Cratons) remained virtually unaffected by tectono-orogenic events, while the southeastern mass is composed of smaller cratonic fragments, which later took part in the formation of two large supercontinents: Rodinia during the Mesoproterozoic, and Gondwana in the Neoproterozoic. (author)

  17. The energy geopolitics interests: the Initiative for South American Regional Integration - ISARI; Os interesses da geopolitica energetica: a Iniciativa para Integracao Regional Sul Americana - IIRSA

    Energy Technology Data Exchange (ETDEWEB)

    Jeronymo, Alexandre; Roig, Carla de Almeida; Guerra, Sinclair Mallet Guy [Universidade Federal do ABC (UFABC), Santo Andre, SP (Brazil). Programa de Pos-Graduacao em Energia], e-mail: alexandre.jeronymo@ufabc.edu.br, e-mail: carla.roig@ufabc.edu.br, e-mail: sguerra@ufabc.edu.br

    2008-07-01

    This work adds itself to the debates on the project Integration of South America Regional Infrastructure - ISARI. Its focus is related with Energy Geopolitics and the South America integration under the world system of commodities production. The ISARI, as a project oriented to the viability of low value aggregated which should be transported from the south american continent for consumption at the world market.

  18. The challenge for integration of South American focusing the energy; O desafio da integracao sul-americana com enfoque a energia

    Energy Technology Data Exchange (ETDEWEB)

    Boeira, Rogerio Londero [Universitetet i Stavanger (Norway)]. E-mail: rlboeira@uol.com.br

    2006-07-01

    The challenge for integration in the South American continent is huge, but feasible as one takes technical decisions instead of political ones. The technique here is so spoken as the one responsible to be the most capable issue to guarantee mercantile actions that objective the investment return. It is not only with this return that it is possible to keep the investment attractiveness for the investors. It also requires the establishment of well-defined and clear rules, as well as of the transparency priority in all the energy market transactions. Political actions for the integration, as the ones that have been taken in the beginning of this century, promote the Idea of the union. However, they are confused and vague as they try to explain how and mainly how much the implementation of such union will cost to the public capital. This present work shows the lack of clearness and discrepancy in the energy commodities supply and demand data in the countries of South America, which would be vital for the implementation of any energy project. The importance of this work is to create consciousness in the society. Like that, it will sustain that political decision for energy investment will not be taken without a clear purpose to foment the society development at a defined cost. (author)

  19. Três novas espécies sul-americanas de Cryptachaea e acréscimos taxonômicos em Achaearanea (Araneae, Theridiidae

    Directory of Open Access Journals (Sweden)

    Erica Helena Buckup

    2012-06-01

    Full Text Available Três espécies de Cryptachaea Archer, 1946 são descritas, duas delas para o Brasil: C. amazonas sp. nov. da Reserva Florestal Adolpho Ducke, Manaus, Amazonas e Cryptachaea maldonado sp. nov. da Base de Operações Geólogo Pedro de Moura, Porto Urucu, rio Urucu, Coari, Amazonas, com base em machos. Uma nova associação de macho e fêmea é proposta para Achaearanea hieroglyphica (Mello-Leitão, 1940. O macho da Guiana Francesa, atribuído à última espécie, é considerado uma espécie nova de Cryptachaea, C. ingijonathorum. O macho de Achaearanea tingo Levi, 1963 é descrito pela primeira vez. Novas ocorrências são listadas para A. trapezoidalis (Taczanowski, 1873.

  20. Avaliação da interação de compostos ativos hidratantes com modelo de biomembrana de Crotalus durissus por meio de calorimetria exploratória diferencial e espectroscopia RAMAN

    Directory of Open Access Journals (Sweden)

    MICHELE TAKAOKA

    2010-06-01

    Full Text Available O objetivo foi verificar as alterações no estrato córneo em modelos alternativos de membrana após a aplicação de ativos hidratantes envolvendo métodos biofísicos. O modelo de biomembrana utilizado foi a muda de pele de Crotalus durissus e os ativos hidratantes foram: uréia, silício orgânico, extrato vegetal de Imperata cylindrica, reação de xilitol e glicose e componentes de NMF. Os resultados da avaliação das alterações do modelo por meio de Espectroscopia Raman com Transformada de Fourier sugerem que os ativos hidratantes confirmam segurança necessária, pois não alteraram de forma acentuada a estrutura do estrato córneo. Utilizando-se Calorimetria Exploratória Diferencial pode-se indicar que a solução de silício orgânico e o gel hidrofílico com uréia apresentaram melhor poder hidratante. Palavras-chave: Estrato córneo. Hidratação. Métodos biofísicos. Crotalus durissus. FT-Raman, DSC. ABSTRACT Study of the interaction of moisturizers with a Crotalus durissus biomembrane by differential scanning calorimetry and RAMAN spectroscopy The objective of this research was to use biophysical techniques to investigate the alterations induced in a biomembrane model of the stratum corneum by the application of moisturizers. The biomembrane was obtained from the skin shed by the rattlesnake Crotalus durissus and the active moisturizing compounds were: urea, dimethylsilanol hyaluronate, Imperata cylindrical plant extract, carbohydrates and natural moisturizing factors (NMF components. Results from FT-Raman spectroscopy suggested that the moisturizers were safe, since they did not promote modifications in the structure of the stratum corneum. Differential scanning calorimetry results indicated that the solution containing the organic silicon compound and the gel with urea showed the best hydrating effects on the stratum corneum. Keywords: Stratum corneum. Hydration. Biophysical techniques. Crotalus durissus

  1. Pulmonary, microbiological and hematological changes in Crotalus durissus terrificus (Serpentes, Viperidae parasitized by nematodes of the genus Rhabdias (Nematoda, Rhabdiasidae Alterações Pulmonares, microbiológicas e hematológicas em Crotalus durissus terrificus (Serpentes, Viperidae parasitadas pelos nematódeos do gênero Rhabdias (Nematoda, Rhabdiasidae

    Directory of Open Access Journals (Sweden)

    K.R. Santos

    2008-06-01

    Full Text Available This study reported the pulmonary, microbiological, and hematological alterations in Crotalus durissus terrificus parasitized by nematodes of the genus Rhabdias. Histological, microbiological, and hematological analysis were performed on parasitized (n=6 and non-parasitized (n=6 snakes. Granulocytic and mononuclear cell infiltrates in the pulmonary parenchyma and epithelium were also observed during the histological analysis of parasitized snakes. Microbiological analysis of parasitized animals revealed the following Gram-negative bacteria: Citrobacter divergens, Burkholderia cepacia, Stenotrophomonas maltophilia, Proteus vulgaris, Enterobacter sakazakii, Enterobacter ammnigenus, Pseudomonas aeruginosa, Pantoea sp. and Providencia rettgeri. In non-parasitized snakes, the following species were identified: B. cepacia, Pseudomonas fluorescens, and Acinetobacter baumanii. Increased levels of plasmatic protein, decreased lymphocyte counts, and normal red blood cell values were observed in parasitized animals.Este trabalho relata as alterações pulmonares, microbiológicas e hematológicas em Crotalus durissus terrificus parasitadas pelo nematódeo do gênero Rhabdias. As análises histológicas, microbiológicas e hematológicas foram realizadas em serpentes parasitadas (n=6 e não parasitadas (n=6. Foram observados infiltrados de células granulocíticas e mononucleares no parênquima pulmonar durante a análise histopatológica das serpentes parasitadas. A análise microbiológica revelou as seguintes bactérias Gram-negativas; Citrobacter divergens, Burkholderia cepacia, Stenotrophomonas maltophilia, Proteus vulgaris, Enterobacter sakazakii, Enterobacter ammnigenus, Pseudomonas aeruginosa, Pantoea sp. e Providencia rettgeri. Nas serpentes não parasitadas foram identificadas: B. cepacia, Pseudomonas fluorescens e Acinetobacter baumanii. Nos animais parasitados observaram-se: aumento da concentração de proteína plasmática, diminuição da

  2. Phospholipase A2 isolated from the venom of Crotalus durissus terrificus inactivates dengue virus and other enveloped viruses by disrupting the viral envelope.

    Directory of Open Access Journals (Sweden)

    Vanessa Danielle Muller

    Full Text Available The Flaviviridae family includes several virus pathogens associated with human diseases worldwide. Within this family, Dengue virus is the most serious threat to public health, especially in tropical and sub-tropical regions of the world. Currently, there are no vaccines or specific antiviral drugs against Dengue virus or against most of the viruses of this family. Therefore, the development of vaccines and the discovery of therapeutic compounds against the medically most important flaviviruses remain a global public health priority. We previously showed that phospholipase A2 isolated from the venom of Crotalus durissus terrificus was able to inhibit Dengue virus and Yellow fever virus infection in Vero cells. Here, we present evidence that phospholipase A2 has a direct effect on Dengue virus particles, inducing a partial exposure of genomic RNA, which strongly suggests inhibition via the cleavage of glycerophospholipids at the virus lipid bilayer envelope. This cleavage might induce a disruption of the lipid bilayer that causes a destabilization of the E proteins on the virus surface, resulting in inactivation. We show by computational analysis that phospholipase A2 might gain access to the Dengue virus lipid bilayer through the pores found on each of the twenty 3-fold vertices of the E protein shell on the virus surface. In addition, phospholipase A2 is able to inactivate other enveloped viruses, highlighting its potential as a natural product lead for developing broad-spectrum antiviral drugs.

  3. Mating Systems, Reproductive Success, and Sexual Selection in Secretive Species: A Case Study of the Western Diamond-Backed Rattlesnake, Crotalus atrox

    Science.gov (United States)

    Clark, Rulon W.; Schuett, Gordon W.; Repp, Roger A.; Amarello, Melissa; Smith, Charles F.; Herrmann, Hans-Werner

    2014-01-01

    Long-term studies of individual animals in nature contribute disproportionately to our understanding of the principles of ecology and evolution. Such field studies can benefit greatly from integrating the methods of molecular genetics with traditional approaches. Even though molecular genetic tools are particularly valuable for species that are difficult to observe directly, they have not been widely adopted. Here, we used molecular genetic techniques in a 10-year radio-telemetric investigation of the western diamond-backed rattlesnake (Crotalus atrox) for an analysis of its mating system and to measure sexual selection. Specifically, we used microsatellite markers to genotype 299 individuals, including neonates from litters of focal females to ascertain parentage using full-pedigree likelihood methods. We detected high levels of multiple paternity within litters, yet found little concordance between paternity and observations of courtship and mating behavior. Larger males did not father significantly more offspring, but we found evidence for size-specific male-mating strategies, with larger males guarding females for longer periods in the mating seasons. Moreover, the spatial proximity of males to mothers was significantly associated with reproductive success. Overall, our field observations alone would have been insufficient to quantitatively measure the mating system of this population of C. atrox, and we thus urge more widespread adoption of molecular tools by field researchers studying the mating systems and sexual selection of snakes and other secretive taxa. PMID:24598810

  4. An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus

    Directory of Open Access Journals (Sweden)

    Daniela de Oliveira Toyama

    2014-01-01

    Full Text Available This paper shows the results of quercitrin effects on the structure and biological activity of secretory phospholipase (sPLA2 from Crotalus durissus terrificus, which is the main toxin involved in the pharmacological effects of this snake venom. According to our mass spectrometry and circular dichroism results, quercetin was able to promote a chemical modification of some amino acid residues and modify the secondary structure of C. d. terrificus sPLA2. Moreover, molecular docking studies showed that quercitrin can establish chemical interactions with some of the crucial amino acid residues involved in the enzymatic activity of the sPLA2, indicating that this flavonoid could also physically impair substrate molecule access to the catalytic site of the toxin. Additionally, in vitro and in vivo assays showed that the quercitrin strongly diminished the catalytic activity of the protein, altered its Vmax and Km values, and presented a more potent inhibition of essential pharmacological activities in the C. d. terrificus sPLA2, such as its myotoxicity and edematogenic effect, in comparison to quercetin. Thus, we concluded that the rhamnose group found in quercitrin is most likely essential to the antivenom activities of this flavonoid against C. d. terrificus sPLA2.

  5. Humoral response and neutralization capacity of sheep serum inoculated with natural and Cobalt 60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

    Energy Technology Data Exchange (ETDEWEB)

    Netto, D.P.; Alfieri, A.A. [Universidade Estadual de Londrina, PR (Brazil). Centro de Ciencias Agrarias, Dept. de Medicina Veterinaria Preventiva; Chiacchio, S.B.; Bicudo, P.L. [UNESP, SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia; Nascimento, N. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Supervisao de Radiobiologia]. E-mail: rnetto@uel.br

    2002-07-01

    The aim of this work was to investigate antigen irradiation on crotalic antivenom and the capacity of sheep as serum producers. Twelve sheep in two groups of six were inoculated with Crotalus durissus terrificus venom. One group was inoculated with natural venom (N V) and the other with Cobalt 60 gamma-irradiated venom (Ir V). Three antigen doses were given to the animals at monthly intervals for immunization. The toxic activity of the venom was assessed by LD 50 determination in mice. Blood samples were collected weekly analyses of serum neutralization capacity and potency. At the end of the experiment, the animals were challenged with a LD 50 for sheep showed no signs of envenoming. These results showed that toxicity of the irradiated venom was 4.4 times less than the natural venom. The sera from the irradiated group neutralized LD 50 14.6 times, and the sera from the natural group 4.4 times. Sera from the irradiated group were five times more potent. The two groups did not present clinical alterations. The results of this study show the potential for using sheep in crotalic antivenom production. The use of irradiated venom in sheep immunization induces a powerful and lasting humoral immune response shown by both the in vitro neutralization and potency tests and by the indirect ELISA antibody level detection technique. (author)

  6. cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake

    Directory of Open Access Journals (Sweden)

    F.H.R. Fagundes

    2010-03-01

    Full Text Available To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR, we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49, from the venom of Crotalus durissus collilineatus (Cdc PLA2. The cDNA encoding Cdc PLA2 from whole venom was sequenced. The deduced amino acid sequence of this cDNA has high overall sequence identity with the group II PLA2 protein family. Cdc PLA2 has 14 cysteine residues capable of forming seven disulfide bonds that characterize this group of PLA2 enzymes. Cdc PLA2 was isolated using conventional Sephadex G75 column chromatography and reverse-phase high performance liquid chromatography (RP-HPLC. The molecular mass was estimated using matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF mass spectrometry. We tested the neuromuscular blocking activities on chick biventer cervicis neuromuscular tissue. Phylogenetic analysis of Cdc PLA2 showed the existence of two lines of N6-PLA2, denominated F24 and S24. Apparently, the sequences of the New World’s N6-F24-PLA2 are similar to those of the agkistrodotoxin from the Asian genus Gloydius. The sequences of N6-S24-PLA2 are similar to the sequence of trimucrotoxin from the genus Protobothrops, found in the Old World.

  7. Preliminary X-ray crystallographic studies of a tetrameric phospholipase A{sub 2} formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom

    Energy Technology Data Exchange (ETDEWEB)

    Marchi-Salvador, D. P.; Corrêa, L. C.; Salvador, G. H. M.; Magro, A. J. [Departamento de Física e Biofísica, Instituto de Biociências, UNESP, CP 510, 18618-000 Botucatu-SP (Brazil); Oliveira, C. Z. [Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, FCFRP, USP, Ribeirão Preto-SP (Brazil); Iulek, J. [Departamento de Química, UEPG, Ponta Grossa-PR (Brazil); Soares, A. M. [Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, FCFRP, USP, Ribeirão Preto-SP (Brazil); Fontes, M. R. M., E-mail: fontes@ibb.unesp.br [Departamento de Física e Biofísica, Instituto de Biociências, UNESP, CP 510, 18618-000 Botucatu-SP (Brazil)

    2007-12-01

    Crotoxin B is a basic phospholipase A{sub 2} found in the venom of C. durissus terrificus and is one of the subunits that constitute crotoxin. Here, the crystallization, X-ray diffraction data collection and molecular-replacement solution of a novel tetrameric complex formed by two dimers of crotoxin B isoforms are presented. Crotoxin B is a basic phospholipase A{sub 2} found in the venom of Crotalus durissus terrificus and is one of the subunits that constitute crotoxin. This heterodimeric toxin, which is the main component of C. d. terrificus venom, is completed by an acidic, nontoxic and non-enzymatic component (crotoxin A) and is involved in important envenomation effects, such as neurological disorders, myotoxicity and renal failure. Although crotoxin was first crystallized in 1938, no crystal structure is currently available for crotoxin, crotoxin A or crotoxin B. In this work, the crystallization, X-ray diffraction data collection to 2.28 Å resolution and molecular-replacement solution of a novel tetrameric complex formed by two dimers of crotoxin B isoforms (CB1 and CB2) is presented.

  8. Effect of Mikania glomerata (Asteraceae) leaf extract combined with anti-venom serum on experimental Crotalus durissus (Squamata: Viperidae) envenomation in rats.

    Science.gov (United States)

    Floriano, Rafael Stuani; Nogueira, Rosa Maria Barilli; Sakate, Michiko; Laposy, Cecília Braga; da Motta, Yudney Pereira; Sangiorgio, Fabíola; David, Heloísa Costa; Nabas, João Marcelo

    2009-12-01

    Crotalic envenomation represents the highest number of deaths when compared to other snakebite envenomations of medical interest. Crotalic venom has important characteristics such as neurotoxicity, myotoxicity, nephrotoxicity, and clotting and hemolytic action. We evaluated the clinical and laboratory aspects of Crotalus durissus terrificus experimental envenomation in Wistar rats treated with antivenom and the aqueous extract of the plant Mikania glomerata. The animals were divided into three groups: Group C (control); Group VS-venom and antivenom; Group VSM-venom, antivenom and aqueous extract of M. glomerata. Crotalic poison caused clinical and laboratory alterations in Wistar mice. Significant clinical alterations were: temperature decrease, edema in the venom inoculated member, sedation and a locomotion decrease in groups VS and VSM when compared with group C. A faster recovery from sedation was observed only for animals of group VSM when compared to VS. There was an increase in the number of leukocytes, neutrophils and creatine kinase in the VS and VSM groups, compared to group C. Wistar rats showed a high resistance to crotalic venom. Additional studies with different doses, time of treatment, different administration methods and histopathological and immunological studies are necessary to understand the action of M. glomerata in crotalic accidents.

  9. Effects of N-acetyl-L-cysteine on redox status and markers of renal function in mice inoculated with Bothrops jararaca and Crotalus durissus terrificus venoms.

    Science.gov (United States)

    Barone, Juliana Marton; Frezzatti, Rodrigo; Silveira, Paulo Flavio

    2014-03-01

    Renal dysfunction is an important aggravating factor in accidents caused by Crotalus durissus terrificus (Cdt) and Bothrops jararaca (Bj) bites. N-acetyl-l-cysteine (NAC) is well known as a nephroprotective antioxidant with low toxicity. The present study investigated the effects of NAC on redox status and markers of renal function in mice that received vehicle (controls) or venoms (v) of Cdt and Bj. In controls NAC promoted hypercreatinemia, hypouremia, hyperosmolality with decreased urea in urine, hyperproteinuria, decreased protein and increased dipeptidyl peptidase IV (DPPIV) in membrane-bound fraction (MF) from renal cortex (RC) and medulla (RM). NAC ameliorated or normalized altered creatinuria, proteinemia and aminopeptidase (AP) acid in MF, AP basic (APB) in soluble fraction (SF), and neutral AP in SF and MF from RC and RM in vBj envenomation. NAC ameliorated or normalized altered neutral AP in SF from RC and RM, and DPPIV and protein in MF from RC in vCdt envenomation. NAC ameliorated or restored renal redox status respectively in vCdt and vBj, and normalized uricemia in both envenomations. These data are promising perspectives that recommend the clinical evaluation of NAC as potential coadjuvant in the anti venom serotherapy for accidents with these snake's genera.

  10. Rabdomióilise secundária a acidente ofídico crotálico (Crotalus durissus terrificus

    Directory of Open Access Journals (Sweden)

    Renato Almeida Magalhães

    1986-08-01

    Full Text Available Relatam-se dois casos de rabdomiólise secundária a envenenamento produzido por Crotalus durissus terrificus. O diagnóstico da rabdomiólise baseou-se na mialgia intensa e generalizada apresentada pelos pacientes e na constatação de níveis séricos elevados de CPK, TGO e DHL. A confirmação do diagnóstico foi obtida no caso n.° 2 pela detecção de mioglobina no soro através de imunoeletroforese contra soro antimioglobina humana e por biópsia muscular. Esse paciente desenvolveu também, como complicação do envenenamento ofídico, quadro clínico e laboratorial de insuficiência renal aguda. Essa complicação foi atribuída à ação nefrotóxica e hemolítica do veneno crotálico e à hipotensão arterial apresentada pelo paciente, não se afastando a possibilidade de que a rabdomiólise tenha sido um fator contribuinte para a sua instalação. Foram constatadas hipocalcemla, hiperuricemia e hiperfosfatemia grave na fase oligúria da insuficiência renal aguda, alterações peculiares quando essa condição está associada à rabdomiólise.

  11. Sulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom

    Directory of Open Access Journals (Sweden)

    Camila L. Pires

    2013-08-01

    Full Text Available Sulfated polysaccharides derived from seaweed have shown great potential for use in the development of new drugs. In this study, we observed that a low-molecular-weight sulfated polysaccharide from Caulerpa racemosa, termed CrSP, could interact with secretory phospholipase A2 (sPLA2 isolated from Crotalus durissus terrificus venom. When native sPLA2 (14 kDa was incubated with CrSP, they formed a molecular complex (sPLA2:CrSP with a molecular mass of 32 kDa, approximately. Size exclusion chromatography experiments suggested that CrSP formed a stable complex with sPLA2. We belived that sPLA2 and SPCr are involved an ionic interaction between negatively charged CrSP and the positively charged basic amino acid residues of sPLA2, because this interaction induced significant changes in sPLA2 enzymatic and pharmacological activities. CrSP caused a significant increase in sPLA2 enzymatic and bactericidal activity and increased its edematogenic effect. A pharmacological assay showed that the myotoxic activity of sPLA2:CrSP is unrelated to its enzymatic activity and that sPLA2:CrSP may have a practical application as a natural antibacterial agent for use in humans and commercially raised animals.

  12. Phospholipase A2 isolated from the venom of Crotalus durissus terrificus inactivates dengue virus and other enveloped viruses by disrupting the viral envelope.

    Science.gov (United States)

    Muller, Vanessa Danielle; Soares, Ricardo Oliveira; dos Santos, Nilton Nascimento; Trabuco, Amanda Cristina; Cintra, Adelia Cristina; Figueiredo, Luiz Tadeu; Caliri, Antonio; Sampaio, Suely Vilela; Aquino, Victor Hugo

    2014-01-01

    The Flaviviridae family includes several virus pathogens associated with human diseases worldwide. Within this family, Dengue virus is the most serious threat to public health, especially in tropical and sub-tropical regions of the world. Currently, there are no vaccines or specific antiviral drugs against Dengue virus or against most of the viruses of this family. Therefore, the development of vaccines and the discovery of therapeutic compounds against the medically most important flaviviruses remain a global public health priority. We previously showed that phospholipase A2 isolated from the venom of Crotalus durissus terrificus was able to inhibit Dengue virus and Yellow fever virus infection in Vero cells. Here, we present evidence that phospholipase A2 has a direct effect on Dengue virus particles, inducing a partial exposure of genomic RNA, which strongly suggests inhibition via the cleavage of glycerophospholipids at the virus lipid bilayer envelope. This cleavage might induce a disruption of the lipid bilayer that causes a destabilization of the E proteins on the virus surface, resulting in inactivation. We show by computational analysis that phospholipase A2 might gain access to the Dengue virus lipid bilayer through the pores found on each of the twenty 3-fold vertices of the E protein shell on the virus surface. In addition, phospholipase A2 is able to inactivate other enveloped viruses, highlighting its potential as a natural product lead for developing broad-spectrum antiviral drugs.

  13. Immunohistochemical changes in kidney glomerular and tubular proteins caused by rattlesnake (Crotalus vegrandis venom Cambios inmunohistoquímicos en proteínas de túbulo y glomérulo renal causadas por el veneno de la serpiente de cascabel (Crotalus vegrandis

    Directory of Open Access Journals (Sweden)

    María E. Girón

    2003-10-01

    Full Text Available Renal damage is an important cause of death in patients who have survived the early effects of severe crotalid envenomation. Extracellular matrix of renal tissue is altered by Crotalus toxin activities. The aim of this study was to describe how cytoskeletal proteins and basal membrane components undergo substantial alterations under the action of Crotalus vegrandis crude venom and its hemorrhagic fraction (Uracoina-1 in mice. To detect the proteins in question, the immunoperoxidase method with monoclonal and polyclonal antibodies was used. Cell types within renal lesions were characterized by phenotypic identification, by means of immunohistologic analysis of marker proteins using different primary antibodies against mesangial cells, endothelial cells, cytoskeletal proteins (intermediate filament, extracellular matrix and basal membranes. Samples for morphological study by standard procedures (biotin-streptavidin-peroxidase technique using light microscopy were processed. Positive and negative controls for each antigen tested in the staining assay were included. After crude venom and hemorrhagic fraction inoculation of mice, the disappearance of cytoskeletal vimentin and desmin and collagen proteins in the kidney was observed. In extracellular matrix and basal membranes, collagen type IV from envenomed animals tends to disappear from 24 h to 120 h after venom injection.El daño renal es una causa importante de muerte en pacientes que sobreviven a los efectos iniciales de los severos envenenamientos crotálicos. El objetivo de este estudio ha sido el describir como las proteínas del citoesqueleto y los componentes de membrana basal muestran alteraciones importantes en su manifestación, bajo la acción del veneno crudo de Crotalus vegrandis y una fracción hemorrágica (Uracoina-1 del mismo veneno ya que, la matriz extracelular del tejido renal es alterada por la actividad de estas toxinas. Para detectar las proteínas en cuestión se utiliz

  14. Evidence for a respiratory component, similar to mammalian respiratory sinus arrhythmia, in the heart rate variability signal from the rattlesnake, Crotalus durissus terrificus.

    Science.gov (United States)

    Campbell, Hamish A; Leite, Cleo A C; Wang, Tobias; Skals, Marianne; Abe, Augusto S; Egginton, Stuart; Rantin, F Tadeu; Bishop, Charles M; Taylor, Edwin W

    2006-07-01

    Autonomic control of heart rate variability and the central location of vagal preganglionic neurones (VPN) were examined in the rattlesnake (Crotalus durissus terrificus), in order to determine whether respiratory sinus arrhythmia (RSA) occurred in a similar manner to that described for mammals. Resting ECG signals were recorded in undisturbed snakes using miniature datalogging devices, and the presence of oscillations in heart rate (fh) was assessed by power spectral analysis (PSA). This mathematical technique provides a graphical output that enables the estimation of cardiac autonomic control by measuring periodic changes in the heart beat interval. At fh above 19 min(-1) spectra were mainly characterised by low frequency components, reflecting mainly adrenergic tonus on the heart. By contrast, at fh below 19 min(-1) spectra typically contained high frequency components, demonstrated to be cholinergic in origin. Snakes with a fh >19 min(-1) may therefore have insufficient cholinergic tonus and/or too high an adrenergic tonus acting upon the heart for respiratory sinus arrhythmia (RSA) to develop. A parallel study monitored fh simultaneously with the intraperitoneal pressures associated with lung inflation. Snakes with a fhrate (fv). Adrenergic blockade by propranolol infusion increased the variability of the ventilation cycle, and the oscillatory component of the fh spectrum broadened accordingly. Infusion of atropine to effect cholinergic blockade abolished this HF component, confirming a role for vagal control of the heart in matching fh and fv in the rattlesnake. A neuroanatomical study of the brainstem revealed two locations for vagal preganglionic neurones (VPN). This is consistent with the suggestion that generation of ventilatory components in the heart rate variability (HRV) signal are dependent on spatially distinct loci for cardiac VPN. Therefore, this study has demonstrated the presence of RSA in the HRV signal and a dual location for VPN in the

  15. Wound healing activity and mechanisms of action of an antibacterial protein from the venom of the eastern diamondback rattlesnake (Crotalus adamanteus.

    Directory of Open Access Journals (Sweden)

    Ramar Perumal Samy

    Full Text Available Basic phospholipase A2 was identified from the venom of the eastern diamondback rattlesnake. The Crotalus adamanteus toxin-II (CaTx-II induced bactericidal effects (7.8 µg/ml on Staphylococcus aureus, while on Burkholderia pseudomallei (KHW, and Enterobacter aerogenes were killed at 15.6 µg/ml. CaTx-II caused pore formation and membrane damaging effects on the bacterial cell wall. CaTx-II was not cytotoxic on lung (MRC-5, skin fibroblast (HEPK cells and in mice. CaTx-II-treated mice showed significant wound closure and complete healing by 16 days as compared to untreated controls (**P<0.01. Histological examination revealed enhanced collagen synthesis and neovascularization after treatment with CaTx-II versus 2% Fusidic Acid ointment (FAO treated controls. Measurement of tissue cytokines revealed that interleukin-1 beta (IL-1β expression in CaTx-II treated mice was significantly suppressed versus untreated controls. In contrast, cytokines involved in wound healing and cell migration i.e., monocyte chemotactic protein-1 (MCP-1, fibroblast growth factor-basic (FGF-b, chemokine (KC, granulocyte-macrophage colony-stimulating factor (GM-CSF were significantly enhanced in CaTx-II treated mice, but not in the controls. CaTx-II also modulated nuclear factor-kappa B (NF-κB activation during skin wound healing. The CaTx-II protein highlights distinct snake proteins as a potential source of novel antimicrobial agents with significant therapeutic application for bacterial skin infections.

  16. Arterial acid-base status during digestion and following vascular infusion of NaHCO(3) and HCl in the South American rattlesnake, Crotalus durissus.

    Science.gov (United States)

    Arvedsen, Sine K; Andersen, Johnnie B; Zaar, Morten; Andrade, Denis; Abe, Augusto S; Wang, Tobias

    2005-12-01

    Digestion is associated with gastric secretion that leads to an alkalinisation of the blood, termed the "alkaline tide". Numerous studies on different reptiles and amphibians show that while plasma bicarbonate concentration ([HCO(3)(-)](pl)) increases substantially during digestion, arterial pH (pHa) remains virtually unchanged, due to a concurrent rise in arterial PCO(2) (PaCO(2)) caused by a relative hypoventilation. This has led to the suggestion that postprandial amphibians and reptiles regulate pHa rather than PaCO(2). Here we characterize blood gases in the South American rattlesnake (Crotalus durissus) during digestion and following systemic infusions of NaHCO(3) and HCl in fasting animals to induce a metabolic alkalosis or acidosis in fasting animals. The magnitude of these acid-base disturbances were similar in magnitude to that mediated by digestion and exercise. Plasma [HCO(3)(-)] increased from 18.4+/-1.5 to 23.7+/-1.0 mmol L(-1) during digestion and was accompanied by a respiratory compensation where PaCO(2) increased from 13.0+/-0.7 to 19.1+/-1.4 mm Hg at 24 h. As a result, pHa decreased slightly, but were significantly below fasting levels 36 h into digestion. Infusion of NaHCO(3) (7 mmol kg(-1)) resulted in a 10 mmol L(-1) increase in plasma [HCO(3)(-)] within 1 h and was accompanied by a rapid elevation of pHa (from 7.58+/-0.01 to 7.78+/-0.02). PaCO(2), however, did not change following HCO(3)(-) infusion, which indicates a lack of respiratory compensation. Following infusion of HCl (4 mmol kg(-1)), plasma pHa decreased by 0.07 units and [HCO(3)(-)](pl) was reduced by 4.6 mmol L(-1) within the first 3 h. PaCO(2), however, was not affected and there was no evidence for respiratory compensation. Our data show that digesting rattlesnakes exhibit respiratory compensations to the alkaline tide, whereas artificially induced metabolic acid-base disturbances of same magnitude remain uncompensated. It seems difficult to envision that the central and

  17. The comparison between the humoral response and the neutralizing capacity of sheep serum inoculated with natural venom and Co{sup 60} irradiated venom from Crotalus durissus terrificus (Laurenti, 1768)

    Energy Technology Data Exchange (ETDEWEB)

    Netto, D.P. [Universidade Estadual de Londrina, PR (Brazil). Centro de Ciencias Agrarias. Dept. de Medicina Veterinaria Preventiva]. E-mail: mnetto@uel.br

    2000-07-01

    Crotalus durissus terrificus venom was irradiated with Co{sup 60} to investigate the effects of antigen-irradiation on antivenom production in sheep. Twelve sheep were divided in two groups of 6. One group received irradiated, while the other received natural venom. Three doses of antigen were given at monthly intervals. The toxic activity of the venom was assessed by LD{sub 50} in mice. Weekly blood samples were obtained to evaluate anti-crotalic serum titers by indirect ELISA, neutralization capacity, and serum potency. A complete blood count, plasma protein and fibrinogen concentration, and serum albumin and globulin were also determined. At end of the experiment, the animals were challenged with ovine LD{sub 50}, without clinical abnormalities. (author)

  18. Rabbit IgG antibodies against Phospholipase A2 from Crotalus durissus terrificus neutralize the lethal activity of the venom Los anticuerpos IgG de conejos anti-fosfolipasa A2 de Crotalus durissus terrificus neutralizan la actividad letal del veneno

    Directory of Open Access Journals (Sweden)

    Juan P. Rodríguez

    2006-12-01

    Full Text Available Crotalus durissus terrificus (C.d.t. (South American rattlesnake venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2 and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75. Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 µg with Freund adjuvant. Groups of six mice (20 + 2 g were inoculated with 0.5 ml i.p. of C. d. t. venom (4 µg or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms.El veneno de Crotalus durissus terrificus (C.d.t. (Cascabel de Sud América posee actividad miotóxica y neurotóxica, actividades que también exhibe el complejo crotoxina, principal componente tóxico de este veneno. El complejo crotoxina está constituido por una fosfolipasa A2 básica (PLA2 y una proteína acídica no tóxica, el crotapotín. En este trabajo se estudió la capacidad neutralizante de anticuerpos IgG anti-PLA2 sobre la letalidad inducida por el veneno entero. El antígeno PLA2, fue aislado por cromatografía de filtración en gel (Sephadex G-75. Se inocularon conejos machos por vía subcutánea e intramuscular, con 700 µg de PLA2 y adyuvante para la obtención de anticuerpos específicos. La capacidad neutralizante del

  19. A low-cost method to test cytotoxic effects of Crotalus vegrandis (Serpentes: Viperidae venom on kidney cell cultures Un método de bajo costo para probar los efectos citotóxicos del veneno de Crotalus vegrandis (Serpentes: Viperidae en cultivos de células renales

    Directory of Open Access Journals (Sweden)

    María E. Girón

    2005-06-01

    Full Text Available The pathogenesis of the renal lesion upon envenomation by snakebite has been related to myolysis, hemolysis, hypotension and/or direct venom nephrotoxicity caused by the venom. Both primary and continuous cell culture systems provide an in vitro alternative for quantitative evaluation of the toxicity of snake venoms. Crude Crotalus vegrandis venom was fractionated by molecular exclusion chromatography. The toxicity of C. vegrandis crude venom, hemorrhagic, and neurotoxic fractions were evaluated on mouse primary renal cells and a continuous cell line of Vero cells maintained in vitro. Cells were isolated from murine renal cortex and were grown in 96 well plates with Dulbecco's Modified Essential Medium (DMEM and challenged with crude and venom fractions. The murine renal cortex cells exhibited epithelial morphology and the majority showed smooth muscle actin determined by immune-staining. The cytotoxicity was evaluated by the tetrazolium colorimetric method. Cell viability was less for crude venom, followed by the hemorrhagic and neurotoxic fractions with a CT50 of 4.93, 18.41 and 50.22 µg/mL, respectively. The Vero cell cultures seemed to be more sensitive with a CT50 of 2.9 and 1.4 µg/mL for crude venom and the hemorrhagic peak, respectively. The results of this study show the potential of using cell culture system to evaluate venom toxicity.La patogénesis de la lesion renal ha sido relacionada a la miolisis, hemólisis, hipotensión y/o el efecto directo del veneno. Tanto el cultivo primario o el cultivo celular continuo proveen una alternativa in vitro para la evaluación cuantitativa de la toxicidad de venenos de serpiente. El veneno crudo de Crotalus vegrandis fue fraccionado por una cromatografía de exclusión molecular. La toxicidad del veneno crudo de C. vegrandis, sus fracciones hemorrágicas y neurotóxicas fueron evaluadas en células renales primarias de ratón y una línea continua de células Vero mantenidas in vitro. Las c

  20. Quadros clínico-patológicos do envenenamento ofídico por Crotalus durissus terrificus e Bothrops spp. em animais de produção

    Directory of Open Access Journals (Sweden)

    Carlos Hubinger Tokarnia

    2014-04-01

    Full Text Available Foi realizada uma revisão dos quadros clínico-patológicos causados pelos venenos de Crotalus durissus terrificus e Bothrops spp. em bovinos, búfalos, ovinos equinos e suínos. Foram compilados os dados obtidos pela experimentação em animais de produção encontrados na literatura e os obtidos através de experimentação realizada por nossa equipe. Também foram revisados os casos naturais de envenenamento ofídico comunicados. Em dois Quadros foram lançados os mais importantes dados dessas revisões, que revelou diversos aspectos interessantes: 1 em nossos experimentos, o veneno de Crotalus durissus terrificus, quando injetado por via subcutânea em cavalos, causou um edema acentuado no local da aplicação, ao contrário do que tem sido observado em todas as outras espécies animais, aspecto não relatado na literatura; 2 em nossos experimentos, o veneno de diversas espécies de Bothrops, quando injetado por via subcutânea em bovinos, ovinos e equinos, não causou edema como em geral é relatado na literatura, e sim hemorragias subcutâneas acentuadas no local da aplicação. Nos casos não fatais este sangue era reabsorvido em poucos dias sem deixar sequelas. Exceção foi a reação ao veneno de Bothrops jararacussu, que causou edema nos ovinos experimentais, e tumefação acentuada que resultou em fístula com eliminação de líquido seroso nos equinos experimentais. O objetivo do presente estudo visa contribuir para o aperfeiçoamento do diagnóstico de acidentes ofídicos em animais de produção.

  1. Crotoxin in humans: analysis of the effects on extraocular and facial muscles Crotoxina em humanos: estudo da ação em músculos extraoculares e faciais

    Directory of Open Access Journals (Sweden)

    Geraldo de Barros Ribeiro

    2012-12-01

    Full Text Available PURPOSE: Crotoxin is the main neurotoxin of South American rattlesnake Crotalus durissus terrificus. The neurotoxic action is characterized by a presynaptic blockade. The purpose of this research is to assess the ability of crotoxin to induce temporary paralysis of extraocular and facial muscles in humans. METHODS: Doses of crotoxin used ranged from 2 to 5 units (U, each unit corresponding to one LD50. We first applied 2U of crotoxin in one of the extraocular muscles of 3 amaurotic individuals to be submitted to ocular evisceration. In the second stage, we applied crotoxin in 12 extraocular muscles of 9 patients with strabismic amblyopia. In the last stage, crotoxin was used in the treatment of blepharospasm in another 3 patients. RESULTS: No patient showed any systemic side effect or change in vision or any eye structure problem after the procedure. The only local side effects observed were slight conjunctival hyperemia, which recovered spontaneously. In 2 patients there was no change in ocular deviation after 2U crotoxin application. Limitation of the muscle action was observed in 8 of the 12 applications. The change in ocular deviation after application of 2U of crotoxin (9 injections was in average 15.7 prism diopters (PD. When the dose was 4U (2 applications the change was in average 37.5 PD and a single application of 5U produced a change of 16 PD in ocular deviation. This effect lasted from 1 to 3 months. Two of the 3 patients with blepharospasm had the hemifacial spasm improved with crotoxin, which returned after 2 months. CONCLUSIONS: This study provides data suggesting that crotoxin may be a useful new therapeutic option for the treatment of strabismus and blepharospasm. We expect that with further studies crotoxin could be an option for many other medical areas.OBJETIVO: A crotoxina é a principal neurotoxina da cascavel sul-americana Crotalus durissus terrificus e sua ação neurotóxica caracteriza-se por um bloqueio pr

  2. Study of crotoxin on the induction of paralysis in extraocular muscle in animal model Estudo da crotoxina na indução de paralisia da musculatura extraocular em modelo animal

    Directory of Open Access Journals (Sweden)

    Geraldo de Barros Ribeiro

    2012-10-01

    crotoxina é a principal toxina do veneno da cobra cascavel sul-americana Crotalus durissus terrificus e causa bloqueio da neurotransmissão na junção neuromuscular. O objetivo deste estudo foi avaliar a ação e aplicabilidade da crotoxina na indução de paralisia da musculatura extrínseca ocular, e comparar seus efeitos com os da toxina botulínica do tipo A (TB-A. MÉTODOS: A crotoxina, com DL50 de 1,5 µg, foi aplicada no músculo reto superior direito de dez coelhos da raça neozelandesa, em concentrações que variaram de 0,015 µg a 150 µg. Em dois coelhos, utilizou-se 2 unidades de toxina botulínica do tipo A para análise comparativa. A avaliação da paralisia foi realizada através de eletromiografia seriada. Após a recuperação, que ocorreu em dois meses, seis coelhos foram sacrificados para estudo anátomopatológico. RESULTADOS: Os animais não apresentaram sinais de intoxicação sistêmica. Ptose palpebral transitória foi observada em quase todos os animais e permaneceu por até 14 dias. As toxinas causaram um bloqueio imediato da captação dos potenciais elétricos. A recuperação foi gradativa no período aproximado de um mês, observando-se sinais evidentes de regeneração no registro eletromiográfico. Os efeitos da crotoxina na paralização do músculo injetado foram proporcionais à concentração. A crotoxina, na concentração de 1,5 µg, induziu alterações semelhantes às da toxina botulínica do tipo A. Os achados anátomo-patológicos foram localizados somente na região em que se aplicou as toxinas, não havendo necrose de fibras musculares em nenhuma amostra analisada. As alterações causadas pela crotoxina também foram proporcionais à concentração utilizada e similares a toxina botulínica do tipo A na concentração de 1,5 µg. CONCLUSÃO: A crotoxina foi capaz de induzir paralisia transitória do músculo reto superior. Este efeito foi caracterizado pela redução na amplitude dos potenciais de ação e sinais inespec

  3. The system of dispute settlement in MERCOSUL: considerations about the South American energy issue; O sistema de resolucao de disputas do MERCOSUL: consideracoes acerca da questao energetica sul-americana

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Pedro Lucas de Moura [Agencia Nacional do Petroleo, Gas Natural e Biocombustiveis (ANP), Brasilia, DF (Brazil). Programa de Recursos Humanos em Direito do Petroleo, Gas Natural e Biocombustiveis

    2009-08-15

    With the advent of globalization, the current tendency is for countries to join in to economic blocs, as such, there is a mutual strengthening. In this context, as this model is developed giving rise to blocks such as MERCOSUL or the European Union, it is necessary that within the block is created a dispute settlement system, marked by the pacifism of their decisions. In this study, we try to show, in general, the operation of the MERCOSUL dispute settlement from the viewpoint of the need for proper functioning of this system and its operation on issues involving energy. For this, we analyzed their normative basements, focusing on the Brasilia Protocol and the Protocol of Olivos, which created and reformulated, respectively, the dispute settlement system of the MERCOSUL.

  4. Tecnologias de informação e integração regional: desafios institucionais para a cooperação Sul-Americana na área de segurança

    Directory of Open Access Journals (Sweden)

    Marco Cepik

    2011-01-01

    Full Text Available Dans cet article, on examine le rôle des Technologies de l'Information et de la Communication (TIC dans le processus d'intégration de la région sud-américaine, à partir de l'étude des problèmes de sécurité régionale et des processus de coopération entre États. On cherche ainsi à voir la portée des TIC dans la construction d'une confiance mutuelle entre les États et dans la syntonie entre le maintien de l'ordre domestique et la stabilité régionale dans un décor marqué par la méfiance et par des restrictions politiques et institutionnelles. On montre que l'usage des TIC peut contribuer à renforcer les processus politiques de la région, bien que, pratiquement, il n'existe pas de stratégie coordonnée pour l'application de ces technologies par les pays d'Amérique du Sud. Le développement des TIC promu par l' UNASUL et par le Conseil de Défense Sud-Américain (CDS semble donc un effort de promouvoir une adéquation entre les initiatives d'intégration observées entre les pays sud-américains et de plus grands échanges d'information en vue de la sécurité et de la stabilité pour la région.

  5. Lamprosoma W. Kirby (Coleoptera, Chrysomelidae: descrição de nova espécie, redescrições e chave para algumas espécies sul americanas

    Directory of Open Access Journals (Sweden)

    Caxambú Marcelo Galeazzi

    2003-01-01

    Full Text Available Lamprosoma alacre sp. nov. from Mato Grosso is described and illustrated. The genus Lamprosoma is redescribed and illustrations of characters are provided. The following south american species are redescribed: L. amethystinum Perty, 1832, L. azureum Germar, 1824, L. bicolor W. Kirby, 1818, L. chrysopygium Germar, 1824, L. corruscum Guérin-Méneville, 1844, L. podtiaguini Monrós, 1947 and L. triste Guérin-Méneville, 1844.

  6. Comparison of humoral immune response, neutralization capacity of anticrotalic serum in young ovines, clinical and weight evaluation between animals inoculated with Crotalus durissus terrificus venom, natural or Cobalt-60-irradiated

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira Junior, R.S. E-mail: rseabra@cevap.org.br

    2005-07-01

    The Elisa technique was used to evaluate and compare the humoral immune response of young ovine to anticrotalic serum production. During serum production, the clinical and weight evaluation of the animals was performed. The parameters utilized were complete blood count, and dosage of urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin. The animals weight was verified fortnightly during the experiment. The neutralization capacity of the serum produced from the snake Crotalus durissus terrificus natural (NV) and Cobalt-60-irradiated venom (IrV) was evaluated by in vitro challenges. One group of six animals received natural venom, the second group received irradiated venom, and the third was the control group. The animals received six immunizations during 84 days with an interval of 14 days. There was a significant difference (p<5%) in the ELISA test for the profile of the antibodies produced by the experimental groups (NV

  7. Nowhere to Go but Up: Impacts of Climate Change on Demographics of a Short-Range Endemic (Crotalus willardi obscurus) in the Sky-Islands of Southwestern North America.

    Science.gov (United States)

    Davis, Mark A; Douglas, Marlis R; Webb, Colleen T; Collyer, Michael L; Holycross, Andrew T; Painter, Charles W; Kamees, Larry K; Douglas, Michael E

    2015-01-01

    Biodiversity elements with narrow niches and restricted distributions (i.e., 'short range endemics,' SREs) are particularly vulnerable to climate change. The New Mexico Ridge-nosed Rattlesnake (Crotalus willardi obscurus, CWO), an SRE listed under the U.S. Endangered Species Act within three sky islands of southwestern North America, is constrained at low elevation by drought and at high elevation by wildfire. We combined long-term recapture and molecular data with demographic and niche modeling to gauge its climate-driven status, distribution, and projected longevity. The largest population (Animas) is numerically constricted (N = 151), with few breeding adults (Nb = 24) and an elevated inbreeding coefficient (ΔF = 0.77; 100 years). Mean home range (0.07 km2) is significantly smaller compared to other North American rattlesnakes, and movements are within, not among sky islands. Demographic values, when gauged against those displayed by other endangered/Red-Listed reptiles [e.g., Loggerhead Sea Turtle (Caretta caretta)], are either comparable or markedly lower. Survival rate differs significantly between genders (female

  8. Nowhere to Go but Up: Impacts of Climate Change on Demographics of a Short-Range Endemic (Crotalus willardi obscurus in the Sky-Islands of Southwestern North America.

    Directory of Open Access Journals (Sweden)

    Mark A Davis

    Full Text Available Biodiversity elements with narrow niches and restricted distributions (i.e., 'short range endemics,' SREs are particularly vulnerable to climate change. The New Mexico Ridge-nosed Rattlesnake (Crotalus willardi obscurus, CWO, an SRE listed under the U.S. Endangered Species Act within three sky islands of southwestern North America, is constrained at low elevation by drought and at high elevation by wildfire. We combined long-term recapture and molecular data with demographic and niche modeling to gauge its climate-driven status, distribution, and projected longevity. The largest population (Animas is numerically constricted (N = 151, with few breeding adults (Nb = 24 and an elevated inbreeding coefficient (ΔF = 0.77; 100 years. Mean home range (0.07 km2 is significantly smaller compared to other North American rattlesnakes, and movements are within, not among sky islands. Demographic values, when gauged against those displayed by other endangered/Red-Listed reptiles [e.g., Loggerhead Sea Turtle (Caretta caretta], are either comparable or markedly lower. Survival rate differs significantly between genders (female

  9. Nowhere to Go but Up: Impacts of Climate Change on Demographics of a Short-Range Endemic (Crotalus willardi obscurus) in the Sky-Islands of Southwestern North America

    Science.gov (United States)

    Davis, Mark A.; Douglas, Marlis R.; Webb, Colleen T.; Collyer, Michael L.; Holycross, Andrew T.; Painter, Charles W.; Kamees, Larry K.; Douglas, Michael E.

    2015-01-01

    Biodiversity elements with narrow niches and restricted distributions (i.e., ‘short range endemics,’ SREs) are particularly vulnerable to climate change. The New Mexico Ridge-nosed Rattlesnake (Crotalus willardi obscurus, CWO), an SRE listed under the U.S. Endangered Species Act within three sky islands of southwestern North America, is constrained at low elevation by drought and at high elevation by wildfire. We combined long-term recapture and molecular data with demographic and niche modeling to gauge its climate-driven status, distribution, and projected longevity. The largest population (Animas) is numerically constricted (N = 151), with few breeding adults (Nb = 24) and an elevated inbreeding coefficient (ΔF = 0.77; 100 years). Mean home range (0.07km2) is significantly smaller compared to other North American rattlesnakes, and movements are within, not among sky islands. Demographic values, when gauged against those displayed by other endangered/Red-Listed reptiles [e.g., Loggerhead Sea Turtle (Caretta caretta)], are either comparable or markedly lower. Survival rate differs significantly between genders (female

  10. Energy integration experiences at the Europe, at the Nordic countries and at the Central America: considerations relevant to the South America integration process; Experiencias de integracao energetica na Europa, nos paises nordicos e na America Central: consideracoes relevantes ao processo de integracao Sul-Americana

    Energy Technology Data Exchange (ETDEWEB)

    Campos, Adriana Fiorotti; Andreza, Fernanda Marques Pereira; Soares, Jeferson Borghetti; Pinheiro, Maria Fernanda Bacile; Oliveira, Ricardo Gorini de [Empresa de Pesquisa Energetica (EPE), Rio de Janeiro, RJ (Brazil)], Emails: adriana.campos@epe.gov.br, fernanda.andreza@epe.gov.br; jeferson.soares@epe.gov.br, maria.pinheiro, ricardo.gorini@epe.gov.br

    2010-07-01

    In view of institutional/contractual regulatory problems at the South America, some experiences of energy integration at the electrical sectors and natural gas (Nordic countries. European Union and Central America), identifying related vantages and advantageous. Besides, there is an attempt of characterization of the process step of energy integration, and the fitting of regions in these steps, observing that the process of energy integration in the South America it is found in a initial step yet if it is considered the used methodology by the Colombian enterprise Interconexion Electrica S.A.E.S.P. - ISA (2007)

  11. On Dichelyne (Cucullanellus elongatus (Tornquist, 1931 Petter, 1974: South American correlated species (Nematoda, Cucullanidae and some other helminths of micropogonias furnieri (Desmarest, 1823 (Pisces, Sciaenidae Sobre Dichelyne (Cucullanellus elongatus (Tornquist, 1931 Petter, 1974: espécies sul americanas correlatas (Nematoda, Cucullanidae e alguns outros helmintos de Micropogonias furnieri (Desmarest, 1823(Pisces, Sciaenidae

    Directory of Open Access Journals (Sweden)

    J. Júlio Vicente

    1989-09-01

    Full Text Available Dichelyne (Cucullanellus elongatus (Tomquist, 1931 Petter, 1974, from Venezuela, of which D.(C. amaurincai (Freitas, Vicente & Ibáñez, 1969 Petter, 1974 is proposed as a junior synonym, is redescribed and comments on the present status of the remaining species under the group, namely D.(C. dichelyneformes (Szidat, 1950 Petter, 1974, D.(C. rodriguesi (Pinto, Fábio & Noronha, 1970 Petter, 1974 and D.(C. travassosi (Guimarães & Cristofaro, 1974, occuring in South America are made. Dollfusentis chandleri Golvan, 1969 (Acanthocephala, Illiosentidae, Poecilancistrium caryophyllum (Diesing, 1850(Cestoidea, Otobothriidae and Callitetrarhynchus gracilis (Rudolphi, 1819(Cestoidea, Dasyrhynchidae as well as larval forms of Echinocephalus sp. (Nematoda, Gnathostomatidae and Contracaecum sp. (Nematoda, Anisakidae are also reported.Dichelyne (Cucullanellus elongatus (Tornquist, 1931 Petter, 1974, da Venezuela, da qual D.(C. amaurincai (Freitas, Vicente & Ibáñez, 1969 Petter, 1974 é proposta como sinônimo junior, é descrita, e são feitos comentários sobre a atual situação das outras espécies do mesmo grupo, ocorrendo na América do Sul, a saber: D.(C. dichelyneformes (Szidat, 1950 Petter, 1974, D.(C. rodriguesi (Pinto, Fábio & Noronha, 1971 Petter, 1974 e D.(C. travassosi (Guimarães & Cristofaro, 1974. Dollfusentis chandleri Golvan, 1969 (Acantochephala, Illiosentidae, Poecilancistrium cariophyllum (Diesing, 1850 (Cestoidea, Otobothriidae e Callitetrarhynchus gracilis (Rudolphi, 1819 (Cestoidea, Dayrhynchidae bem como formas larvares de Exhinocephalus sp. (Nematoda, Gnathostomatidae e Contracaecum sp. (Nematoda, Anisakidae são também assinaladas.

  12. Sistemática, filogenia e distribuição geográfica das espécies sul-americanas de Centris (Paracentris Cameron, 1903 e de Centris (Penthemisia Moure, 1950, incluindo uma análise filogenética do "grupo Centris" sensu Ayala, 1998 (Hymenoptera, Apoidea, Centridini

    Directory of Open Access Journals (Sweden)

    Fernando César Vieira Zanella

    2002-01-01

    Full Text Available Systematics, phylogeny and geographical distribution of the South American species of Centris (Paracentris Cameron, 1903, and Centris (Penthemisia Moure, 1950, including a phylogenetic analysis of the "Centris group" sensu Ayala, 1998 (Hymenoptera, Apoidea, Centridini. A cladistic analysis with the objective of testing the hypothesis of monophily of Centris (Paracentris Cameron, 1903, and of studying its phylogenetic relationships with the other subgenera that belong to the Centris group, sensu Ayala, 1998, and the relationships among the species that occur in South America, is presented. Centris (Paracentris is a group of New World bees of amphitropical distribution, especially diversified in the Andes and in the xeric areas of South and North America. Thirty-one species were included in the analysis, four considered as outgroup, and 49 characters, all from external morphology and genitalia of adult specimens. Parsimony analyses with equal weights for the characters and successive weighting were performed with the programs NONA and PAUP, and analyses of implied weighting with the program PeeWee. The strict consensus among the trees obtained in all the analyses indicates that C. (Paracentris, as previously recognized, is a paraphyletic group. In order to eliminate that condition, the subgenera C. (Acritocentris, C. (Exallocentris and C. (Xerocentris, all described by SNELLING (1974 are synonymized under C. (Paracentris. The subgenus C. (Penthemisia Moure, 1950, previously considered a synonym of C. (Paracentris, is reinstated, but in a more restricted sense than originally proposed and with the following species: Centris brethesi Schrottky, 1902; C. buchholzi Herbst, 1918; C. chilensis (Spinola, 1851, C. mixta mixta Friese, 1904, and C. mixta tamarugalis Toro & Chiappa, 1989. Centris mixta, previously recognized as the only South American species of the subgenus C. (Xerocentris, a group supposedly amphitropical, came out as the sister-species of C. buchholzi. The following South American species were recognized under Centris (Paracentris: Centris burgdorfi Friese, 1901; C. caelebs Friese, 1900; C. cordillerana Roig-Alsina, 2000; C. euphenax Cockerell, 1913; C. flavohirta Friese, 1900; C. garleppi (Schrottky, 1913; C. klugii Friese, 1900; C. lyngbyei Jensen-Haarup, 1908; C. mourei Roig-Alsina, 2000; C. neffi Moure, 2000; C. nigerrima (Spinola, 1851; C. toroi sp. nov.; C. tricolor Friese, 1900; C. unifasciata (Schrottky, 1913, and C. vogeli Roig-Alsina, 2000. The relationships among the subgenera of the "Centris group" were: (Xanthemisia (Penthemisia (Centris s. str. - Paracentris. Centris xanthomelaena Moure & Castro 2001, an endemic species of the Caatinga and previously considered a C. (Paracentris, came out as the sister group of C. (Centris s. str. A new species of C. (Paracentris from Chile is described: Centris toroi sp. nov. Lectotypus designations and redescriptions are presented for Centris burgdorfi, C. caelebs, C. lyngbyei, C. tricolor, C. autrani Vachal, 1904 and C. smithii Friese, 1900. New synonyms proposed: C. buchholzi Herbst, 1918 = Centris wilmattae Cockerell, 1926 syn. nov.; C. caelebs Friese, 1900 = Paracentris fulvohirta Cameron, 1903. The female of C. vogeli Roig-Alsina, 2000 and the male of C. xanthomelaena are described.

  13. Universidades populares en España y su relación con la universidad suramericana Universidades populares em Espanha e sua relação com a universidade sul-americana Popular Universities in Spain and their Relationship with South American Universities

    Directory of Open Access Journals (Sweden)

    Juan Antonio López-Núñez

    2009-03-01

    Full Text Available El trabajo presenta las claves para entender el Proyecto Universidad Popular, iniciado en España en 1901. Tras analizar brevemente su pasado, se explica su presente a través de la educación de adultos como filosofía de fondo, y se hace ver que en la actualidad no deja de estar vigente en nuestra sociedad. La principal nota característica de este proyecto es su organización en red, no solo a nivel nacional, sino que sus relaciones con otras universidades populares, sobre todo de Suramérica, constituyen su principal fuente de trabajo. A pesar de sus más de cien años de existencia en Europa y Suramérica, en la actualidad el proyecto es toda una institución de educación de adultos, por su peculiar oferta formativa, totalmente adaptada a las necesidades de sus alumnos.Este trabalho apresenta a clave para compreender o Projeto Universidade Popular, que começou em Espanha em 1901. Depois analisar seu passado, explica-se seu presente através da educação de adultos como filosofia de fundo, e descobre-se que na atualidade é em vigor na nossa sociedade. A característica principal deste projeto é sua organização em rede nacional. Suas relações com outras universidades populares, especialmente da América do Sul, são sua fonte de trabalho fundamental. No entanto sua antigüidade de mais de cem anos em Europa e América do Sul, na atualidade o projeto é uma instituição de educação de adultos por sua particular forma de oferta de formação, adaptada as necessidades dos alunos.This study outlines the aspects that are crucial to understanding the Popular University Project begun in Spain in 1901. After a brief historical analysis, its present situation is explained against the backdrop of adult education as an in-depth philosophy, and the project is shown to be valid even today, in our society. The primary feature of the project is its organization in the form of a network, not only nationwide, but in terms of its relationship with other popular universities, particularly those in South America, which constitute its main source of work. Although it was established more than one hundred years ago in Europe and South America, the project continues to be an institution for adult education that offers an unusual form of training adapted entirely to the needs of its students.

  14. Insuficiência renal aguda secundária a acidentes ofídicos botrópico e crotálico. Análise de 63 casos

    Directory of Open Access Journals (Sweden)

    Carlos Faria Santos Amaral

    1986-08-01

    Full Text Available Sessenta e três pacientes com insuficiência renal aguda secundária a acidente ofídico foram tratados no CTI do Hospital das Clínicas da UFMG. Em 32 pacientes (51% o acidente foi produzido por serpentes do gênero Bothrops (grupo bio-trópico e em 32 pacientes (49% pela cascavel sul-americana (grupo crotálico. As principais complicações apresentadas pelos pacientes foram a uremia (100% dos casos, hiperpotassemia (89% dos casos, anemia (78% dos casos, infecção urinária (37% dos casos, hiper-hidratação (17% dos casos, parada cardíaca (14% dos casos e edema agudo dos pulmões (11% dos casos. Cinco pacientes do grupo crotálico (16% tiveram insuficiência respiratória aguda atribuída à ação neurotóxica do veneno, quatro dos quais se recuperaram completamente. Sete pacientes do grupo botrópico (22% tiveram necrose cortical renal diagnosticada em cinco através da biópsia renal e em dois na necropsia. Quarenta e cinco pacientes (71% foram tratados com diálise peritoneal e a hemodiálise foi necessária em dois pacientes, um dos quais havia sido submetido a diálise peritoneal. Em 17 pacientes (27% o tratamento foi conservador. Cinqüenta e cinco pacientes receberam alta hospitalar, quatro dos quais com insuficiência renal crônica secundária a necrose cortical renal e oito (13% faleceram. Os óbitos foram atribuídos a edema pulmonar agudo em quatro pacientes, a estado de choque em dois pacientes e a coma e infecção respiratória após parada cardíaca em dois pacientes.

  15. Desenvolvimento e aplicação de metodologia por cromatografia em camada delgada para determinação do perfil de alcalóides oxindólicos pentacíclicos nas espécies sul-americanas do gênero Uncaria Development and application of a thin layer chromatographic method for the determination of the pentacyclic oxindole alkaloid profile in South-American species of the genus Uncaria

    Directory of Open Access Journals (Sweden)

    Ligia M.M. Valente

    2006-06-01

    Full Text Available O gênero Uncaria (Rubiaceae é representado na América do Sul e Central por duas espécies: U. tomentosa (Willd. DC. e U. guianensis (Aubl. Gmel., conhecidas popularmente como unha-de-gato. Ambas são trepadeiras perenes, sendo empregadas na prevenção e cura de várias doenças. Nessas plantas são encontrados alcalóides oxindólicos e indólicos, triterpenos glicosilados, taninos e flavonóides. Seis alcalóides oxindólicos pentacíclicos, considerados seus marcadores: especiofilina, mitrafilina, uncarina F, isomitrafilina, pteropodina e isopteropodina, são usados na padronização do material vegetal e fitoterápicos derivados. O presente trabalho descreve o desenvolvimento de metodologia analítica qualitativa utilizando cromatografia em camada delgada (CCD para determinação do perfil dos seis alcalóides oxindólicos pentacíclicos marcadores das espécies. O desenvolvimento do método incluiu a comparação entre o uso do extrato metanólico bruto, e de frações enriquecidas obtidas por partição ácido-base clássica ou pelo uso de resina básica Poliamida 6. Utilizou-se gel de sílica como fase estacionária, e variaram-se alguns parâmetros como: eluentes, concentração da amostra, espaço de eluição e tipos de reveladores. O método desenvolvido em CCD mostrou-se confiável, reprodutível e seletivo para os alcalóides alvos, sendo aplicado na análise de amostras de folhas e caule das duas espécies e também de fitoterápicos comerciais à base de U. tomentosa.The species Uncaria tomentosa (Willd. DC. and U. guianensis Gmel. (Rubiaceae, known as cat's claw, are large woody vines occurring in the Amazon rain forest and other tropical areas of South and Central America. It has been used medicinally by indigenous peoples for at least 2,000 years for several diseases. Tetra- and pentacyclic oxindole alkaloids, triterpenoid glycosides, sterols and flavonoids are found in these plants. Among these metabolites, six pentacyclic oxindole alkaloids, speciophylline, mitraphylline, pteropodine, uncarine F, isopteropodine and isomitraphylline, are considered to be the biochemical markers and are used to standardize commercial herbal medicines. The present study describes the development of an analytical methodology to determine the profile of these alkaloid markers through thin layer chromatography (TLC. This development has also included a comparison among the use of the crude methanol extract and fractions obtained through the classical acid-base partition or by using the basic resin Polyamide 6. Silica gel was used as stationary phase with the variation of some parameters such as solvent systems, sample concentration, distance of development and detection method. The TLC method developed was shown to be reliable, reproducible and selective for the target alkaloids. It has been applied to the analysis of leaves and stems from both species as well as phytopharmaceutical derivatives based on U. tomentosa.

  16. Reproduction of a South American population of pumpkinseed sunfish Lepomis gibbosus (Linnaeus (Osteichthyes, Centrarchidae: a comparison with the European and North American populations Reprodução de uma população Sul-Americana de percas-sol Lepomis gibbosus (Linnaeus (Osteichthyes, Centrarchidae: uma comparação com populações européias e norte-americanas

    Directory of Open Access Journals (Sweden)

    André L. B. de Magalhães

    2005-06-01

    Full Text Available The reproductive biology of the introduced pumpkinseed sunfish Lepomis gibbosus (Linnaeus, 1758 in the Custódio's Dam, rio Doce high basin, state of Minas Gerais, Brazil was studied from February/2001 to March/2002 and compared with introduced and native populations. Reproduction occurred almost along the entire period of study with a high frequency of fishes in the advanced ripening/mature and spawned/spent stages. The microscopic analyses indicated that the pumpkinseed sunfish presents multiple spawning. The egg diameter of the Brazilian population is similar to the Spanish, Greek and American ones. The standard length at maturity and the gonadosomatic index of the introduced populations are smaller when compared with native ones. The spawning season of the Brazilian population was the longest among all comparisons made in the present work.Estudou-se de fevereiro/2001 a março/2002 a biologia reprodutiva da exótica perca-sol Lepomis gibbosus (Linnaeus, 1758 na represa do Custódio, bacia do alto rio Doce, Brasil, comparando-a à de populações exóticas e nativas. A reprodução ocorreu praticamente ao longo de todo o período de estudo e a análise microscópica de ovários indicou desova do tipo parcelada para a espécie. O diâmetro ovocitário da população brasileira é semelhante à espanhola, grega e americana. O comprimento padrão na maturidade e o índice gonadossomático de populações introduzidas são menores do que as nativas. O período de desova da população brasileira foi o maior dentre todas as populações analisadas no presente trabalho.

  17. The forked tongue and edge detection in snakes (Crotalus oreganus): an experimental test.

    Science.gov (United States)

    Parker, M Rockwell; Young, Bruce A; Kardong, Kenneth V

    2008-02-01

    Many stimulus-detection systems are lateralized to allow for simultaneous comparison of paired stimuli. It has been hypothesized that the deeply forked tongue of snakes and some derived lizards functions as a chemical edge detector where cues gathered by each tine are kept separate to provide two points of lateral odor assessment by the central nervous system via vomeronasal input. While following a chemical trail, one time can be on the trail, the other off, and such differential information prompts the snake to turn back to the trail. The authors tested this hypothesis in rattlesnakes within a predatory context by unilaterally severing the vomeronasal nerves. If edge detection is used by snakes during prey trailing, then unilateral denervation should disrupt trailing ability. The authors found no change in the seven separate trailing parameters measured. Therefore, they found no support for the edge detection hypothesis as it applies to prey trailing behavior. Instead, the deeply forked tongue may represent a chemosensory specialization to increase odor-sampling area, with snakes and derived lizards detecting only the concentration of chemical trails.

  18. The role of the vomeronasal organ in rattlesnake (Crotalus viridis oreganus) predatory behavior.

    Science.gov (United States)

    Alving, W R; Kardong, K V

    1996-01-01

    During predatory behavior, rattlesnakes depend primarily upon thermal and visual cues to initially aim a strike. However, it has been hypothesized that prey-related odors sensed by the vomeronasal system act as releasing stimuli of the strike and that such vomodors are primary stimuli during poststrike trailing and swallowing of the envenomated rodent. To test this, northern Pacific rattlesnakes were rendered avomic by bilateral lesions of the vomeronasal nerves, and their vomic and avomic predatory behaviors were compared. Avomic rattlesnakes exhibited fewer strikes and complete elimination of trailing and swallowing behavior. These results support the hypothesis that vomodors sensed via the vomeronasal organ are capable of acting as releasing stimuli of selected rattlesnake predatory behaviors. Sensory input via the vomeronasal organ is important during prestrike/strike behavior, and it is a major route of sensory input during poststrike trailing and ingestion of envenomated prey.

  19. Tertiary Structural studies of Myotoxin a from Crotalus viridis viridis Venom by Nuclear Magnetic Resonance

    Science.gov (United States)

    1993-05-01

    electrophorus electri... 47 47 47 Sw:Ibb2SWheat P09864 wheat (triticum aestivum). proteina ... 46 46 51 Sw:Pa29$Pseau P20253 mulga snake (pseudechis...Sw:Pa2bSPsepo P20259 red-bellied black snake (pseudechis... 34 46 37 Sw:IbblSWheat P09863 wheat (triticum aestivum). proteina ... 46 46 52 Sw:Pa2a$Psepo P20258 red

  20. Gopherus agassizii (desert tortoise) and Crotalus ruber (red diamond rattlesnake). Burrow co-occupancy

    Science.gov (United States)

    Lovich, Jeffrey E.

    2011-01-01

    I observed an adult Desert Tortoise and an adult Red Diamond Rattlesnake (sexes unknown) in a shallow tortoise burrow on 6 January 1997 at a wind energy generation facility near Palm Springs, Riverside Co., California, USA (33.9599°N, 116.6613°W).

  1. Notizen zur Fortpflanzungsbiologie und Jugendentwicklung zweier Grubenottern (Serpentes: Crotalidae: Crotalus atrox und Agkistrodon p. piscivorus)

    NARCIS (Netherlands)

    Petzold, Hans Günter

    1963-01-01

    Über die postembryonale Entwicklung — besonders die Gewichtszunahme — junger Giftschlangen (Viperiden, Crotaliden, Elapiden) gibt es aus naheliegenden Gründen nur wenige eingehendere Untersuchungen. KLAUBER (1956) bringt an Hand eines großen Materials eine Zusammenfassung einschlägiger Freilandbeoba

  2. Crystallization and preliminary X-ray crystallographic analysis of two vascular apoptosis-inducing proteins (VAPs) from Crotalus atrox venom

    Energy Technology Data Exchange (ETDEWEB)

    Igarashi, Tomoko; Oishi, Yuko [Department of Cardiac Physiology, National Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565 (Japan); Araki, Satohiko [Sugashima Marine Biological Laboratory, Graduate School of Science, Nagoya University, Toba, Mie 517-0004 (Japan); Mori, Hidezo [Department of Cardiac Physiology, National Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565 (Japan); Takeda, Soichi, E-mail: stakeda@ri.ncvc.go.jp [Department of Cardiac Physiology, National Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565 (Japan); Laboratory for Structural Biochemistry, Riken Harima Institute at SPring-8, 1-1-1 Kouto, Mikazuki, Sayo, Hyogo 679-5148 (Japan)

    2006-07-01

    Vascular apoptosis-inducing protein 1 (VAP1) and VAP2 from C. atrox venom were crystallized in variety of different crystal forms. Diffraction data sets were obtained to 2.5 and 2.15 Å resolution for VAP1 and VAP2, respectively. VAPs are haemorrhagic snake-venom toxins belonging to the reprolysin family of zinc metalloproteinases. In vitro, VAPs induce apoptosis specifically in cultured vascular endothelial cells. VAPs have a modular structure that bears structural homology to mammalian ADAMs (a disintegrin and metalloproteinases). VAP1 is a homodimer with a MW of 110 kDa in which the monomers are connected by a single disulfide bridge. VAP2 is homologous to VAP1 and exists as a monomer with a MW of 55 kDa. In the current study, several crystal forms of VAP1 and VAP2 were obtained using the vapour-diffusion method and diffraction data sets were collected using SPring-8 beamlines. The best crystals of VAP1 and VAP2 generated data sets to 2.5 and 2.15 Å resolution, respectively.

  3. Cloning, expression, and hemostatic activities of a disintegrin, r-mojastin 1, from the mohave rattlesnake (Crotalus scutulatus scutulatus).

    Science.gov (United States)

    Sánchez, Elda E; Lucena, Sara E; Reyes, Steven; Soto, Julio G; Cantu, Esteban; Lopez-Johnston, Juan Carlos; Guerrero, Belsy; Salazar, Ana Maria; Rodríguez-Acosta, Alexis; Galán, Jacob A; Tao, W Andy; Pérez, John C

    2010-09-01

    Interactions with exposed subendothelial extracellular proteins and cellular integrins (endothelial cells, platelets and lymphocytes) can cause alterations in the hemostatic system associated with atherothrombotic processes. Many molecules found in snake venoms induce pathophysiological changes in humans, cause edema, hemorrhage, and necrosis. Disintegrins are low molecular weight, non-enzymatic proteins found in snake venom that mediate changes by binding to integrins of platelets or other cells and prevent binding of the natural ligands such as fibrinogen, fibronectin or vitronectin. Disintegrins are of great biomedical importance due to their binding affinities resulting in the inhibition of platelet aggregation, adhesion of cancer cells, and induction of signal transduction pathways. RT-PCR was used to obtain a 216 bp disintegrin cDNA from a C. s. scutulatus snake venom gland. The cloned recombinant disintegrin called r-mojastin 1 codes for 71 amino acids, including 12 cysteines, and an RGD binding motif. r-Mojastin 1 inhibited platelet adhesion to fibronectin with an IC50 of 58.3 nM and ADP-induced platelet aggregation in whole blood with an IC50 of 46 nM. r-Mojastin 1 was also tested for its ability to inhibit platelet ATP release using PRP resulting with an IC50 of 95.6 nM. MALDI-TOF mass spectrum analysis showed that r-mojastin has a mass of 7.95676 kDa.

  4. O rebote SENDERISTA

    Directory of Open Access Journals (Sweden)

    RICARDO DOS SANTOS POLETTO

    2010-10-01

    Full Text Available Apesar de ser pouco focado pela mídia, o Peru faz-se como um importante ator a ser analisado na conjuntura sul-americana. Marcado pela herança de Sendero Luminoso, o Estado Peruano se vê constantemente ameaçado no que se refere à sua estabilidade e capacidade de auto-sustentação. Nesse sentido o presente artigo busca analisar aos efeitos que o senderismo tem sob a política peruana e sua estabilidade.

  5. Geodinâmica de superfícies de aplanamento, desnudação continental e tectônica ativa como condicionantes da megageomorfologia do Brasil orienta

    Directory of Open Access Journals (Sweden)

    Roberto Célio Valadão

    2009-11-01

    Full Text Available A megageomorfologia do Brasil Oriental é avaliada, neste trabalho, a partir da análise da desnudação continental mesocenozóica, das superfícies de aplanamento elaboradas em razão desse processo e dos soerguimentos crustais a ele associados. Muitos resultados alcançados diferem significativamente daqueles apresentados em trabalhos prévios, particularmente no que se refere à gênese e diversidade das superfícies de aplanamento, ao mecanismo de evolução da paisagem desde a fragmentação do Gondwana-Oeste, e à magnitude e amplitude dos soerguimentos crustais neocenozóicos. A história fanerozóica do relevo do Brasil Oriental foi pontuada por episódios de intensa desnudação e pelo acúmulo de espessa sequência sedimentar em bacias marginais e interiores. Desde o Paleozóico, grande parte da área investigada permaneceu soerguida e em acelerado processo erosivo, particularmente a região em que atualmente se localiza a Serra do Espinhaço mineiro e baiano e a Chapada Diamantina. O processo de individualização da Placa Sul-Americana e abertura do Atlântico Sul, iniciado no Mesozóico, imprimiu novo ritmo à desnudação do continente, cuja justaposição ao caráter episódico dos soerguimentos crustais culminou, na área investigada, na elaboração de três amplas superfícies de aplanamento: (iSuperfície Sul-Americana; (ii Superfície Sul-Americana I; (iii Superfície Sul-Americana II. A elaboração dessas superfícies foi acompanhada pelo recuo do Grande Escarpamento, uma proeminente feição do relevo herdada do processo de rifteamento da margem continental leste brasileira. Esse escarpamento constitui um divisor hidrográfico de expressão regional, separando a bacia do Rio São Francisco, caracterizada pela reduzida capacidade desnudacional, daquelas bacias articuladas diretamente ao nível de base do Oceano Atlântico, mais agressivas.

  6. Actividad hemolítica de venenos de serpientes de los géneros Bothrops, Lachesis, Crotalus y Micrurus(Serpentes: Viperidae y Elapidae)

    OpenAIRE

    Martínez Cadillo, Elba; Bonilla Ferreyra, César; Zavaleta, Alfonso

    2016-01-01

    Desde comienzos de siglo, el efecto hemolítico de los venenos de serpiente atrajo el interés de numerosos investigadores, quienes observaron que ciertas especies del género Naja (familia Elapidae), producían la lisis de los glóbulos rojos humanos y de varios animales, lo que no ocurría frecuentemente con los venenos de especies de otras familias Desde comienzos de siglo, el efecto hemolítico de los venenos de serpiente atrajo el interés de numerosos investigadores, quienes observaron que c...

  7. The unequal influences of the left and right vagi on the control of the heart and pulmonary artery in the rattlesnake, Crotalus durissus

    DEFF Research Database (Denmark)

    Taylor, E.W.; Andrade, Denis V.; Abe, Augusto A.

    2009-01-01

    Autonomic control of the cardiovascular system in reptiles includes sympathetic components but heart rate (fH), pulmonary blood flow ( pul) and cardiac shunt patterns are primarily controlled by the parasympathetic nervous system. The vagus innervates both the heart and a sphincter on the pulmonary...

  8. Anatomia descritiva e comparativa do sistema urinário de Crotalus durissus Linnaeus, 1758, Bothrops neuwiedi Wagler, 1824 e B. moojeni Hoge, 1965 (Ophidia, Viperidae)

    OpenAIRE

    Silva, Luisa Helena Rocha da

    2008-01-01

    As serpentes desenvolveram uma imensa variedade de especializações quanto ao uso do habitat e nicho ocupados ao longo de sua evolução. As diferenças mais notáveis estão na forma, no tamanho do corpo e na textura superficial, o que reflete na diversidade de comportamentos e adaptações ecológicas e fisiológicas. Apesar do número de espécies e por isso mesmo, das diversas possibilidades de estudos que poderiam ser realizados, poucos são os trabalhos abordando a anatomia e fisiologia das serpente...

  9. Male Snakes Allocate Time and Energy according to Individual Energetic Status: Body Condition, Steroid Hormones, and Reproductive Behavior in Timber Rattlesnakes, Crotalus horridus.

    Science.gov (United States)

    Lind, Craig M; Beaupre, Steven J

    2015-01-01

    Life-history theory predicts that organisms will hedge current reproductive investment against potential costs in terms of survivorship and future fecundity. However, little is known regarding the endocrine mechanisms underlying bet-hedging strategies in free-ranging male vertebrates. We examined the relationships among individual energetic status, steroid hormones, mate search, and reproductive behavior in free-ranging male timber rattlesnakes. Snakes were monitored over four active seasons in order to test two hypotheses: (1) males adjust the amount of time and energy allocated toward reproduction according to the level of individual energy stores, and (2) observed condition-dependent reproductive allocation is associated with circulating concentrations of steroid hormones (testosterone and corticosterone) thought to regulate reproductive behaviors in vertebrates. A positive relationship between body condition and testosterone was observed in both the field and the laboratory. Male mate search effort was positively correlated with both body condition and testosterone. Body condition and testosterone concentrations were negatively related to time allocated toward foraging during the breeding season. A strong effect of year was observed in the analysis of testosterone and search effort, suggesting that multiple environmental factors impact hormone production and reproductive investment. Corticosterone was not related to any measured variable. Therefore, our results did not indicate a clear role of corticosterone in mediating observed relationships between energetic status and behavior. Observed relationships are consistent with the hypothesis that males allocate time and energy toward reproduction according to individual energetic status and that testosterone plays a role in mediating the trade-off between current reproductive investment and residual reproductive value.

  10. [First record of Zoophthora radicans (Entomophthorales: Entomophthoraceae) on adults of the Paraguay tea psyllid, Gyropsylla spegazziniana Lizer & Trelles (Hemiptera: Psyllidae), in Brazil].

    Science.gov (United States)

    Alves, Luis F A; Leite, Luis G; Oliveira, Daian G P de

    2009-01-01

    We report the first occurrence of Zoophthora radicans infecting adults of Gyropsylla spegazziniana Lizer & Trelles in a commercial Paraguay tea plantation (Ilex paraguariensis), in Cascavel, PR, Brazil. The fungus prevalence was high (90% of mortality), considered a natural epizooty.

  11. Conmebol não quer eliminatórias aos finais de semana e gera atrito com CBF

    OpenAIRE

    Rizzo, Marcel

    2015-01-01

    A tabela das eliminatórias da Copa-2018 vai gerar atrito entre Conmebol e CBF. A entidade sul-americana informou à Fifa que as partidas, em rodadas duplas, devem ser às quintas e sextas e às segundas e terças - o sorteio dos confrontos será neste sábado (25). Esse calendário esbarra na preferência da Tv Globo, detentora dos direitos de transmissão no país e parceira da CBF, que prefere jogos aos finais de semana, por causa de sua grade. CBF e Globo não comentaram o assunto.

  12. GÊNESE E EVOLUÇÃO DE TURFEIRAS NAS SUPERFÍCIES GEOMÓRFICAS DA SERRA DO ESPINHAÇO MERIDIONAL – MG

    Directory of Open Access Journals (Sweden)

    Márcio Luiz Silva

    2017-03-01

    Full Text Available A Serra do Espinhaço Meridional – SdEM possui litologias predominantemente quartzíticas e é caracterizada por apresentar áreas dissecadas entremeadas a superfícies de aplainamento em diferentes altitudes, onde, nas depressões, ocorrem as turfeiras. Essas superfícies de aplainamentos são resultado de uma sucessão de eventos denudacionais e deposicionais que compartimentou o relevo, ao longo da evolução geodinâmica da Serra. O objetivo desse trabalho foi mapear as turfeiras da porção setentrional da serra e discutir a gênese e evolução desses pedoambientes nas superfícies geomórficas da Serra do Espinhaço Meridional. A determinação da área e mapeamento das turfeiras foi realizada por meio de trabalhos de campo, análises de imagens e de fotografias aéreas. Com o auxílio do vibro-testemunhador coletou-se testemunhos em oito turfeiras. Amostras para datações e caracterização do ciclo fotossintético da cobertura vegetal que colonizou cronologicamente as áreas foram coletadas em diferentes profundidades, para determinação dos isótopos de carbono (δ13C e 14C. A porção norte da SdEM ocupa uma área de 1.180.109,00 ha, onde foram mapeados 14.287,55 ha de turfeiras, o que representa 1,2% da área total. Nas turfeiras predominam os estágios de decomposição avançado (sáprico, seguido do intermediário (hêmico. Quatro níveis de superfícies geomórficas foram identificados em toda a extensão da SdEM: Superfície I (S1, Superfície II (S2, Superfície III (S3 e Superfície IV (S4. Considerando a altimetria como principal fator, a S1 foi cronocorrelacionada com a Superfície Pós-Gondwana e a S2 cronocorrelacionou-se com a Superfície Sul-Americana. A Superfície III (S3 teve correspondência com a Superfície Sul-Americana I e a Superfície IV (S4, por sua vez, com a Superfície Sul-Americana II. A maioria das turfeiras, desenvolvidas no Quaternário, entre o Pleistoceno e Holoceno, se formaram sobre a Superf

  13. DISCURSO POLÍTICO E O SOCIALISMO DO SÉCULO XXI NA AMÉRICA DO SUL

    Directory of Open Access Journals (Sweden)

    Rafael Pinheiro de Araujo

    2015-07-01

    Full Text Available A política sul-americana transformou-se na primeira década do século XXI. Presenciamos um conjunto de processos insurrecionais que fizeram das novas formas de movimentos sociais os protagonistas das lutas na região. Na Bolívia, no Equador e na Venezuela o recrudescimento da luta de classes propiciou a ascensão de governos que expressaram esse novo momento. Evo Morales, Rafael Corrêa e Hugo Chávez simbolizaram a emergência de um novo momento político na América do Sul. Diferentemente de períodos anteriores de luta, nessa nova fase, presenciamos o abandono da luta armada, por meio da utilização da via institucional. Presenciamos, também, a realização das Assembleias Constituintes e o fomento à democracia participativa como instrumentos para as mudanças sociopolíticas. Além disso, assistimos à reivindicação de um novo tipo de socialismo, o Socialismo do Século XXI. Essa proposta começou a ser difundida na Venezuela entre os anos de 2004 e de 2006, com a radicalização da revolução bolivariana. A consolidação do chavismo e o êxito das outras insurreições populares sul-americanas também possibilitaram essa difusão. Realizamos neste artigo uma comparação da construção do Socialismo do Século XXI na Bolívia, Equador e Venezuela, por meio dos discursos políticos e programas de governos ocorridos nesses três processos políticos

  14. Energy inputs and outputs in the wheat production at different localizations; Balanco de energia na producao de trigo em diferentes locais

    Energy Technology Data Exchange (ETDEWEB)

    Gollmann, Pedro; Greco, Marcelo; Campos, Alessandro Torres [Universidade Estadual do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil). Grupo de Pesquisas em Ambiencia do Oeste do Parana], e-mail: jefersonklein@yahoo.com.br; Klein, Jeferson [Universidade Estadual do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil); Palagi, Celso A.; Jurach, Jorge J. [Cooperativa Central de Pesquisa Agricola Tecnologia da Nossa Terra (COODETEC), Cascavel, PR (Brazil)

    2004-07-01

    The intensifying and modernization of agriculture, in spite of improving the productivity, it caused a complete petroleum derived dependence, so much in the form of fuels, lubricants and machinery, as in the form of inputs for the production. This work had as objective to esteem the energy inputs and outputs and the energy conversion, in two different local, in the west of Parana State/Brazil. The largest consumption was observed for the direct energy category, with 95.6% and 95.28% in Cascavel and Palotina, respectively. In the direct energy flow, the Palotina production spent 5,0% more. The consumption of fossil energy was of 22.32% for the Palotina farm and of 23.84% for the Cascavel farm. The energy conversion was of 3.2 and 1.8, for the wheat productions in Cascavel and Palotina, respectively. (author)

  15. Descrição da larva de último estádio de Micrathyria pseudeximia Westfall (Odonata, Libellulidae

    Directory of Open Access Journals (Sweden)

    Marina Schmidt Dalzochio

    2009-08-01

    Full Text Available A larva de último estádio de Micrathyria pseudeximia Westfall, 1992 é descrita e ilustrada a partir de material procedente de Cascavel, Paraná, Brasil. A caracterização do gênero com base nas larvas é ampliada.Description of the Ultimate Stadium Larva of Micrathyria pseudeximia Westfall(Odonata, Libellulidae.Abstract. The larva of ultimate stadium of Micrathyria pseudeximia Westfall, 1992 is described and illustrated based on material collected in Cascavel Municipality, Paraná State, Brazil. The generic concept based on the larvae is amplified.

  16. Assessing the influence of mechanical ventilation on blood gases and blood pressure in rattlesnakes

    DEFF Research Database (Denmark)

    Bertelsen, Mads F.; Buchanan, Rasmus; Jensen, Heidi M.

    2015-01-01

    , randomized trial. ANIMALS: Twenty one fasted adult South American rattlesnakes (Crotalus durissus terrificus). METHODS: Snakes were anesthetized with propofol (15 mg kg(-1) ) intravenously, endotracheally intubated and assigned to one of four ventilation regimens: Spontaneous ventilation, or mechanical...

  17. Instituições Internacionais de Saúde no Contexto Sub-Regional

    Directory of Open Access Journals (Sweden)

    Roberta de Freitas Campos

    2016-03-01

    Full Text Available Ao que pese as diferenças nucleares entre os blocos União Europeia (UE - supranacional e União das Nações Sul-americanas (UNASUL - intergovernamental, em ambos as competências regionais em matéria sanitária não se sobrepõe às competências dos Estados-membros. No caso da União Europeia os art. 4, §2, k; art. 6, a; art. 168 do Tratado sobre o Funcionamento da UE confere apenas poderes para agir em complemento das políticas nacionais; enquanto na União de Nações Sul-americanas, nos artigos 2º e 3º, f de seu Tratado Constitutivo prevê como objetivo construir, de maneira participativa e consensuada, um espaço de integração, para que de forma consequente os Estados independentes possam garantir o acesso universal aos serviços de saúde às suas populações. Dessa forma, o objetivo é verificar as competências, a constituição histórico-regional, objetivos e potencialidades dessas instituições internacionais, criadas no âmbito desses blocos regionais, em influenciar as políticas regionais e nacionais de saúde. Tendo em vista a ampla gama de instituições internacionais que atuam de forma multisetorial na saúde, o estudo apresenta aqueles órgãos cujas competências são estabelecidas nos tratados constitutivos e decisões presidenciais e resoluções desses blocos. Para isso foi realizada uma pesquisa documental e bibliográfica no âmbito das Instituições da União Europeia e da Unasul. Um importante ponto de questionamento diz respeito à existência ou não de um diálogo entre essas instituições e os cidadãos dos blocos a que pertencem, uma vez que se parte do pressuposto que a democracia deve ser uma regra implícita no ‘jogo’ internacional.

  18. Cooperação e integração regional em saúde na América do Sul: a contribuição da Unasul-Saúde Regional cooperation and integration in health in South America: the contribution of Unasur-Health

    Directory of Open Access Journals (Sweden)

    Paulo Marchiori Buss

    2011-06-01

    Full Text Available O objetivo deste artigo é discutir o processo de integração da América do Sul na área da saúde, desde a constituição da União de Nações Sul-Americanas (Unasul, em maio de 2008, até o surgimento do Conselho de Saúde da Unasul, em 2009, e seu ulterior desenvolvimento no último ano (2009-2010. Este estudo é de caráter descritivo e o meio de investigação foi o estudo de caso. Apresentam-se os principais elementos constitutivos da Agenda de Saúde Sul-Americana, com especial destaque para a área de desenvolvimento e gestão de recursos humanos em saúde, que esteve sob a coordenação do Brasil no último ano. Os autores concluem que esse intercâmbio contribuirá para minimizar as assimetrias entre os países, colaborando para a melhoria das condições de vida e saúde da região.The scope of this study is to discuss the process of integration of South America in the domain of health, from the constitution of the Union of South American Nations (Unasur, in May 2008, through to the emergence of the Unasur Council of Health, in 2009, and its ongoing development in the past year (2009-2010. This study is descriptive in nature and the medium of investigation was the case study. The authors present the main constituent elements of the South-American Health Agenda, with particular emphasis on the area of development and management of human resources in health, which was coordinated by Brazil in the past year. The authors conclude that this interchange will contribute to minimization of the asymmetries amongst the countries, collaborating to improve the quality of life and health conditions in the region.

  19. FLUXOS IMIGRATÓRIOS DE LATINO-AMERICANOS: CIDADANIA TRANSNACIONAL

    Directory of Open Access Journals (Sweden)

    Celia Toledo Lucena

    2013-12-01

    Full Text Available A imigração na atualidade tornou-se um fenômeno social e econômico que se apresenta como um problema nas políticas internacionais e às políticas latino-americanas. Assim, a imigração centro e sul americana, sob a perspectiva de gênero, para diferentes lugares do planeta, têm se intensificado a partir de 1990.Com relação ao destino, existem dois tipos migratórios favoritos: as regiões metropolitanas e as regiões de fronteira. Tendo em vista perceber as diferentes formas de integração, em região metropolitana, o grupo de peruanos foi objeto de análise na cidade de São Paulo, na migração trans-fronteiriça, a fronteira Nicarágua-Costa Rica foi espaço de reflexão. A fronteira segue o ritmo das metrópoles e tende a se transformar em território global.

  20. Sacroiliíte bilateral por criptococos: relato de um caso e revisão de literatura Bilateral cryptococcal sacroiliitis: case report and literature review

    Directory of Open Access Journals (Sweden)

    Érika Kameya da Silva

    2006-06-01

    Full Text Available O envolvimento do sistema osteoarticular na criptococose ocorre, particularmente, nas formas disseminadas da infecção, afetando principalmente indivíduos imunodeprimidos. O sistema ósseo é acometido em 5 a 10% dos casos e o sistema articular é raramente comprometido. Até o momento, há 31 casos de artrite criptocócica relatados na literatura médica e, nesta série, apenas 2 casos de sacroiliíte criptocócica. Os autores relatam o caso de um paciente com síndrome da imunodeficiência adquirida (AIDS, que desenvolveu criptococose disseminada, complicada com sacroiliíte bilateral. Este se constitui no primeiro caso de sacroiliíte criptocócica relatado na literatura médica brasileira e sul-americana.The involvement of osseous and articular system in cryptococcosis occurs particularly in the disseminated forms of the infection, affecting mostly immunossupressed hosts. The bone system is compromised in 5 to 10% of the cases, and the articular system is rarely implicated. Until now, there are 31 related cases of cryptococcal arthritis in the medical literature, and in this serie, only 02 cases of criptococcal sacroiliitis. The authors discuss a case of a patient with AIDS that developed disseminated cryptococcosis, complicated with bilateral sacroiliitis. To our knowledge this is the first case of cryptococcal sacroiliitis related in the Brazilian and South-American medical literature.

  1. As meditações americanas de Keyserling: um cosmopolitismo nas incertezas do tempo

    Directory of Open Access Journals (Sweden)

    Daniel Faria

    2013-12-01

    Full Text Available Hoje esquecida, a obra de Keyserling teve grande sucesso nas décadas de 1920 e 1930, com êxito editorial e repercussão entre intelectuais ao redor do mundo. No Brasil, visitado pelo conde em 1929, Keyserling foi citado em textos de Oswald e Mário de Andrade, comentários de Alceu Amoroso Lima e Lindolfo Collor, entre outros. Mário de Andrade chegou a dizer que a obra de Keyserling era a chave para a interpretação de Macunaíma.. O tema de sua obra era a decadência ocidental frente à pluralidade dos tempos históricos nas civilizações do mundo. O oriente lento, o progresso europeu e o primitivismo americano formariam uma harmonia mundial das temporalidades. Neste artigo nos deteremos nas reflexões de Keyserling e seus interlocutores, tendo em vista a questão dos mal-entendidos subjacentes a um projeto de paz mundial, baseado numa interpretação exotizante acerca das diferenças culturais, especialmente as relacionadas a uma suposta identidade sul-americana.

  2. Sistemas de Salud en Suramérica: desafios para la universalidad, la integralidad y la equidad; Ligia Giovanella, Oscar Feo, Mariana Faria, Sebastián Tobar (orgs., ISAGS/UNASUR, Rio de Janeiro, 2012

    Directory of Open Access Journals (Sweden)

    Ana Cris na Souto

    2013-05-01

    Full Text Available O livro aqui apresentado não é resultado apenas de um projeto editorial, mas de um importante avanço que tem ocorrido nos países da América do Sul nesses últimos anos: a criação da União das Nações Sul-americanas (Unasul e do Instituto Sul-americano de Governo em Saúde (Isags, instituição integrante dessa União. Com o propósito de circular conhecimento, realizar debates e análise conjuntural e política, além de buscar o estreitamento dos 12 países membros, o Isags tem realizado importantes eventos. Este livro é fruto de uma de suas iniciativas, a Ofi cina Sistemas de Saúde na América do Sul: desafi os para a universalidade, a integralidade e a equidade, ocorrida em julho de 2011 na cidade do Rio de Janeiro.

  3. La conciencia de la mestiza /towards a new consciousness: uma conversação inter-americana com Gloria Anzaldúa La conciencia de la mestiza/towards a new consciousness: an inter-american conversation with Gloria Anzaldúa

    Directory of Open Access Journals (Sweden)

    Sonia Torres

    2005-12-01

    Full Text Available Este ensaio propõe uma leitura de "La conciencia de la mestiza/Towards a New Consciousness", de Gloria Anzaldúa, entretecida de um diálogo que busca possíveis pontos que unem as modalidades de pensar as identidades chicana e latino-americana, já que a obsessão sul-americana e caribenha pela identidade gerou inúmeros textos, tanto teóricos quanto ficcionais (inclusive os que, como o de Anzaldúa, deslizam entre um e outro gênero, que resistem à polarização (mesmo quando a incorporam, através do reconhecimento de uma cultura complexa, multi-facetada, em que os textos do colonizador/colonizado, opressor/oprimido, cultura dominante/cultura dominada são inextricáveis.This essay proposes a reading of Gloria Anzaldua's "La consciencia de la mestiza/towards a new consciousness", interwoven with a dialogue that looks at possible points linking modalities of thinking Chicana and Latin American identities, given Latin American and Caribbean obsession with identity, which has generated both fictional and theoretical works (as well as those that, like Anzaldua's, conflate the two genres that resist polarization (even while incorporating it, through the recogniztion of a complex, multi-faceted culture in which the texts of the colonized/colonizing, oppressor/oppressed, dominant culture/dominated culture are inextricable.

  4. A arqueologia dos fermentados: a etílica história dos Tupi-Guarani

    Directory of Open Access Journals (Sweden)

    Fernando Ozorio de Almeida

    2015-04-01

    Full Text Available O consumo de bebidas fermentadas é geralmente negligenciado pela literatura arqueológica, que trata a questão como tema de interesse secundário (recreativo na história das populações humanas. Entretanto, a literatura etnográfica das sociedades indígenas das terras baixas sul-americanas indica exatamente o oposto: é o alimento vegetal sólido e não alcoólico que tende a possuir um papel secundário na vida cotidiana e ritualística de diversos coletivos. Os dados arqueológicos aprofundam temporalmente essa relação entre o ser humano e os fermentados. Além disso, os vasos cerâmicos arqueológicos utilizados para o preparo e consumo desses fermentados são fundamentais para a compreensão de processos e eventos históricos que modelaram a dispersão de uma série de grupos pelo continente.

  5. NECESSIDADE DE MAIOR CRÍTICA ÀS FONTES HISTORIOGRÁFICAS SOBRE OS ÍNDIOS NO BRASIL: CONTRIBUIÇÕES À ETNO-HISTÓRIA, ETNOARQUEOLOGIA E À ARQUEOLOGIA PRÉ-HISTÓRICA

    Directory of Open Access Journals (Sweden)

    Rafael Borges Deminicis

    2015-06-01

    Full Text Available As analogias feitas pelo uso de fontes historiográficas (mapas, imagens, manuscritos etc., principalmente as que abordam os povos indígenas encontrados no Brasil, sem uma metodologia crítica de análise dessas fontes, têm criado algumas armadilhas aos estudos nos campos da etno-história e da etnoarqueologia. Portanto, o que poderia auxiliar no descobrimento da história e das identidades étnicas das inúmeras sociedades tribais remotas ou indígenas sul-americanas, traz cada vez mais encobrimento. Neste sentido, uma dialética serial das fontes se faz extremamente necessária. O panorama do que existe acumulado no Brasil sobre métodos de crítica das fontes historiográficas sobre as sociedades indígenas é extremamente raro, resumindo-se a duas referências: o trabalho de síntese dos grandes contextos sociais de produção das fontes criado pelo sociólogo Florestan Fernandes; e o sistema de observação das particularidades das fontes, a “sociologia dos viajantes”, do antropólogo João Pacheco de Oliveira. Um primeiro passo na direção de uma dialética e serial às referidas fontes seria a combinação desses dois métodos.

  6. ANATOMIA DA MADEIRA E CASCA DO ESPINILHO, Acacia caven (Mol. Mol.

    Directory of Open Access Journals (Sweden)

    José Newton Cardoso Marchiori

    1992-12-01

    Full Text Available São descritos os aspectos anatômicos da madeira e casca de Acacia cavem (Mol. Mol. São apresentados dados quantitativos de 34 características do xilema secundário, bem como fotomicrografias das estruturas anatômicas da madeira e casca. A ausência de septos em fibras, a abundância de parênquima axial e a elevada percentagem de raios com 4 ou mais células de largura, são os caracteres mais importantes na estrutura do lenho. O arranjo das fibras floemáticas em feixes tangenciais regulares, rodeados por sériescristalíferas, é, por sua vez, o aspecto mais notável da casca. Este caráter ainda não havia sido reportado pela literatura anatômica das acácias sul-americanas, e pode ter valor taxonômico em nivel infra-genético.

  7. Scenedesmaceae (Chlorophyceae – Chlorococcales) de um lago artificial urbano: Desmodesmus e Scenedesmus - DOI: 10.4025/actascibiolsci.v29i3.483 Scenedesmaceae (Chlorophyceae – Chlorococcales) of an urban artificial lake: Desmodesmus and Scenedesmus - DOI: 10.4025/actascibiolsci.v29i3.483

    OpenAIRE

    Norma Catarina Bueno; Carina Moresco

    2007-01-01

    Trata-se do inventário florístico de Scenedesmaceae no Lago Municipal de Cascavel (24°82’S, 53º28´W), Paraná, Brasil. As amostras foram coletadas na região litorânea, no período de janeiro de 2002 a outubro de 2003. Foram registrados 21 táxons, sendo 13 do gênero Desmodesmus e oito pertencentes ao gênero Scenedesmus. O trabalho apresenta descrição, chave e ilustrações para a identificação das espécies.An inventory of Scenedesmaceae was made in the Lago Municipal de Cascavel (24°82’S, 53º 28´W...

  8. Allelopathy of Camelina sativa Boiss. (Brassicaceae) on germination and early development of Bidens pilosa (L.) and Glycine max (L.) Merr

    OpenAIRE

    Jéssica da Silva; Andréa Maria Teixeira Fortes; Fernanda Melo Gomes; Tassiane Terezinha Pinto; Thaliny Bonamigo; Nayara Parisoto Boiago

    2011-01-01

    This study evaluated the possible presence of allelopathic potential in camelina, as well as its effect on germination and early growth of soybean seedlings and beggarticks, in order, to verify the possibility of cultivating it with soybeans in a culture rotation system and its use as a herbicide. The experiments were carried out at the Laboratory of Plant Physiology of Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil. The design was completely randomized and the evaluations...

  9. Radiographic evaluation of the effect of obesity on alveolar bone in rats with ligature-induced periodontal disease

    Directory of Open Access Journals (Sweden)

    do Nascimento CM

    2013-10-01

    Full Text Available Cassiane Merigo do Nascimento,1 Tiago Cassol,2 Fernanda Soares da Silva,3 Maria Lucia Bonfleur,4 Carlos Augusto Nassar,5 Patricia Oehlmeyer Nassar5 1Biologica Science and Health Center, State University of West Paraná (UNIOESTE, Cascavel, Paraná, Brazil; 2State University of West Paraná (UNIOESTE, Cascavel, Paraná, Brazil; Department of 3Pharmacy, 4Fisiology, 5Periodontology, Dental School, State University of West Paraná (UNIOESTE, Cascavel, Paraná, Brazil Abstract: There is evidence that the lack of metabolic control of obese patients may accelerate periodontitis. The aim of this study was to evaluate radiographically the effect of cafeteria-diet-induced obesity on alveolar bone loss in rats subjected to periodontal disease. Twenty male Wistar rats were randomly divided into four groups: 1 control group, 2 control and ligature group; 3 cafeteria group; and 4 cafeteria and ligature group. The animals were evaluated for obesity and euthanized, and the mandible of each rat was removed to perform a radiographic evaluation of alveolar bone loss and its effect on diet-induced obesity. The results showed greater alveolar bone loss in the mice in Group 4 (P<0.01. Thus, we concluded that obese mice, on average, showed greater radiographic evidence of alveolar bone loss than mice undergoing induction of obesity. Keywords: periodontal disease, radiography, obesity

  10. Management of venomous snakebites in dogs and cats in Brazil

    Directory of Open Access Journals (Sweden)

    R.S. Ferreira Júnior

    2004-01-01

    Full Text Available Snake envenoming is a major problem both to veterinary and human medicine in tropical countries due to high incidence, severity, and sequelae. In Brazil, most envenomings involving animals are caused by Bothrops and Crotalus snakes; these are the highest risk to animals. This study reports on Bothrops and Crotalus envenomings in dogs, the main species responsible for epidemiology, pathogenesis, venom action, clinical signs, sequelae and complications, clinical pathology, necropsy findings, diagnosis, and treatment. Veterinarians must be capable of identifying the snake not only by observing its characteristics but also symptom evolution.

  11. Premissas da história do estabelecimento dos contatos entre o Brasil e a Rússia Premises of the history of the contacts establishment between Brazil and Russia

    Directory of Open Access Journals (Sweden)

    Elena AZ Zhebit

    2010-05-01

    Full Text Available O Imperador Russo e o eminente reformador da Rússia, Pedro, o Grande, "abriu" a Rússia para a Europa e, principalmente, deu aos russos a oportunidade de conhecer o mundo. Várias expedições foram lançadas para explorar novas terras. Neste contexto, surgiram alguns projetos no início do século XIX, estimulando a penetração russa na América Latina. O interesse geopolítico da coroa russa era bastante ambicioso por estabelecer comércio direto com as colônias sul-americanas a fim de facilitar o "abastecimento" da Kamchatka e da América Russa em cereais, rum e açúcar. Esse objetivo foi atingido com o dinheiro da Companhia Russo-Americana, inclusive com os investimentos dos grandes comerciantes russos – membros da Companhia.The Russian Emperor and the eminent Russian reformer Peter, the Great, "opened" Russia to Europe and, basically, gave the Russians a chance to know the world. Several expeditions were launched to discover new lands. In this context, various projects of a Latin American exploration appear at the beginning of the XIXth century, favouring Russian penetration into Latin America. The geo-political interest of the Russian Crown was ambitious enough to establish a direct trade with Southern American colonies with the aim of "contributing" to provide Kamchatka peninsula and the Russian America with grain, rum and sugar. This purpose was achieved by the money of the Russian-American Company, as well as through the investments of big Russian merchants, members of the Company.

  12. Brazilian foreign policy towards South America during the Lula administration: caught between South America and Mercosur Política externa brasileira para a América do Sul durante o governo Lula: entre América do Sul e Mercosul

    Directory of Open Access Journals (Sweden)

    Miriam Gomes Saraiva

    2010-12-01

    Full Text Available The aim of this article is to analyze Brazil's foreign policy towards the South American region during President Lula's administration. As such, the article intends to highlight two specific dimensions: the extent to which foreign policy during this period has differed from previous periods and the relative importance granted by Brazilian diplomacy to recent cooperation and integration efforts, more specifically the Unasur and Mercosur. The article argues that the Lula administration has behaved differently from its predecessors by prioritizing the building up of Brazilian leadership in South America on several different fronts, especially by strengthening multilateral institutions in the regionO objetivo deste artigo é analisar a política externa para a região sul-americana durante o governo de Lula. Assim, no artigo, pretende-se destacar duas dimensões específicas: a extensão pela qual a política externa durante esse período diferenciou-se dos períodos anteriores e a importância relativa dada pela diplomacia brasileira à cooperação e à integração regional, mais especificamente a importância dada à Unasul e ao Mercosul. No artigo, argumenta-se que o governo Lula comportou-se diferentemente do seu predecessor, priorizando a construção de liderança na América do Sul em várias frentes, especialmente fortalecendo instituições regionais

  13. Em busca de uma nova inserção da América Latina na economia global

    Directory of Open Access Journals (Sweden)

    Maria Cristina Cacciamali

    2012-08-01

    Full Text Available O objetivo deste artigo é discutir, de forma interdisciplinar, as condições necessárias e os caminhos para concretizar um modelo econômico e político de maior autonomia no cenário mundial para os países da América do Sul. O enfoque parte das condições políticas e sociais configuradas pela região ao fim da primeira década do presente século, enfatizando o papel do Brasil e considerando as relações econômicas e políticas do contexto internacional, a estabilidade política e o crescente protagonismo da China nos países da região. Analisa ainda aspectos referentes à importância da política social e da política de defesa comum sob a União das Nações Sul-Americanas (Unasul.The aim of this paper is to discuss, in an interdisciplinary way, the necessary conditions and steps to achieve a more autonomous political and economic model on the world stage for South America countries. It departs from the political and social conditions of the region by the end of the first decade of this century. Emphasis is placed on the role of Brazil across the region in the light of the economic and political relations in the international arena, political stability and the growing influence of China. It also examines issues concerning the importance of maintaining an inclusive social policy and a common defense policy under the Southern America Nations Union (Unasur.

  14. As estratégias de cooperação Sul-Sul nos marcos da política externa brasileira de 1993 a 2007 South-South cooperation strategies in Brazilian Foreign Policy from 1993 to 2007

    Directory of Open Access Journals (Sweden)

    Miriam Gomes Saraiva

    2007-12-01

    Full Text Available O objetivo do artigo é analisar as duas vertentes da cooperação sul-sul adotadas pela política externa brasileira entre 1993 e 2007. Por um lado, o artigo examina a política externa brasileira em face da América do Sul: o Mercosul e a cooperação sul-americana em termos mais gerais que, dentro do arco das atuações externas do país, é o tipo de cooperação sul-sul identificada como prioritária. Por outro lado, dedicará também algumas reflexões para a cooperação do Brasil com outros países considerados emergentes e system-affecting, em outros continentes, como é o caso da África do Sul, Índia, China e, em diferente medida, da Rússia.The aim of this article is to analyze the two approaches to South-South cooperation evident in Brazilian foreign policy between 1993 and 2007. On one hand the article examines Brazilian foreign policy towards South America. Specifically, cooperation with Mercosur members and South-American cooperation broadly conceptualized. The region is a foreign policy priority among its south-south cooperation activities. On the other hand, this paper reflects on Brazilian cooperation with other emerging and "system-affecting" countries that belong to other continents, such as South Africa, India and China, and to a certain extent, Russia.

  15. Os relatos do Caminho-Morte: etnografia e tradução de poéticas ameríndias Tales of the Way of Death: ethnography and the translation of Amerindian poetry

    Directory of Open Access Journals (Sweden)

    Pedro de Niemeyer Cesarino

    2012-12-01

    Full Text Available O artigo trata de aspectos da etnografia e da tradução de poéticas ameríndias das terras baixas sul-americanas, em especial do povo Marubo (Vale do Javari, Amazonas. A partir de uma breve revisão de perspectivas teóricas centrais para a compreensão do assunto, busco argumentar que o trabalho de recriação tradutória de cantos e narrativas provenientes de performances orais deve ser realizado em conexão com o trabalho de tradução conceitual. Por fim, apresento a tradução bilíngue de um longo depoimento narrativo sobre o destino póstumo e a formação do Caminho dos Mortos ("Os relatos do Caminho-Morte", oferecido pelo xamã marubo Armando Cherõpapa.The article addresses aspects of the ethnography and the translation of the poetry of Amerindian peoples from the South American lowlands, particularly the Marubo (Javari valley, Amazon. I begin with a brief review of theoretical perspectives that are central to understanding the subject and go on to argue that the work of translational re-creation of songs and narratives derived from oral performances should be carried out in tandem with the work of conceptual translation. Finally, I provide a bilingual rendering of a long narrative testimony on posthumous fate and the formation of the Way of the Dead ("The Reports of the Death-Path", as given by Marubo shaman Armando Cherõpapa.

  16. Diamictites and Sturtian Cap Dolomites Covering the Jacobina Group - Araras, North of Campo Formoso - Bahia

    Directory of Open Access Journals (Sweden)

    Augusto José de Cerqueira Lima Pedreira da Silva

    2008-10-01

    Full Text Available Occurrences of diamictites with a probable glacial origin underlying limestones north of Campo Formoso – BA around Araras village, had already been described by some authors during the 60´s and 70´s. Some of these occurrences have been recorded in some geological maps of 1/100,000 scales and even in others of smaller scales. The importance of these occurrences which have been studied in greater detail is increased because of the now identified litho-structural similarities (and probable stratigraphic correlation with those of Ituaçu, América Dourada, Morro do Chapéu (all of which overlie the ChapadaDiamantina Group, as well as with those in the northern part of the São Francisco Craton (Patamuté, Bendegó etc. overlying TTG rock units of the basement. For all these cases there is evidence of foreland tectonics associated to the centripetal vergence of the Neoproterozoic fold belts, marginal to the craton. There are some additional observations of these rock units occurring in the level of the “Sul-americana Surface”, thus demonstrating deep levels of erosion, which may be partially responsible for the present geographic separation of the whole original litho-stratigraphic context. Nevertheless it seems very clear that these records have somehow been sparsely distributed in the Cryogenian paleogeographic scenery, which may be an additional argument to contest the Snow Ball Earth theory. Some selected geological sections were undertaken in thesurroundings of Araras which made it possible to discriminate 4 types of litho-structural relationships between the Jacobina Group (Orosirian and the overlying diamictites of the São Francisco (Bebedouro Fm. Cryogenian.

  17. Política externa brasileira: as diferentes percepções sobre o Mercosul = Brazilian foreign policy: the dissimilar perceptions about Mercosul

    Directory of Open Access Journals (Sweden)

    Saraiva, Miriam Gomes

    2010-01-01

    Full Text Available A eleição de Lula para a presidência, junto com as eleições de Néstor Kirchner e Tabaré Vásquez, abriu caminho para a construção de expectativas sobre um avanço do Mercosul. No entanto, esta expectativa confirmou-se apenas parcialmente e, pouco a pouco, a dimensão mais sul-americana sob a liderança brasileira foi ocupando um lugar prioritário na política externa brasileira. Suas lacunas passaram a ser então objeto de atenção de estudiosos brasileiros. Com esta preocupação, um dos elementos que limitam seu aprofundamento refere-se às percepções e expectativas diferentes que existem sobre o bloco no interior de seus estados membros, perpassando tanto os diplomatas, burocratas de outras agências governamentais. O artigo avalia o caso do Mercosul acudindo à análise das ideias sobre as quais se fundamentam as iniciativas de integração, tomando como base as ideias dos atores governamentais brasileiros – com destaque para a diplomacia – desde os anos 80 até nossos dias. O artigo é dividido em quatro partes: a apresentação das percepções do processo de integração que permearam o aparato governamental brasileiro; descrição e análise da evolução destas ideias; as ideias presentes no governo de Lula; e as conclusões

  18. A China na América do Sul e as implicações geopolíticas do Consenso do Pacífico

    Directory of Open Access Journals (Sweden)

    Javier Vadell

    2011-11-01

    Full Text Available O artigo analisa as implicações políticas da crescente interdependência econômica entre a República Popular da China (RPC e os países da América do Sul. Apresentam-se dados sobre o comércio e investimento da RPC na sub-região e destacam-se os avanços diplomáticos em matéria de cooperação bilateral no decorrer do século XXI. Parte-se da questão de se estaríamos perante um padrão de relacionamento que poderia constituir uma renovada relação de cooperação Sul-Sul ou, pelo contrário, um novo tipo de relacionamento Norte-Sul. A nossa hipótese é que a evolução e a dinâmica desse relacionamento se assemelham mais a um sistema ou padrão Norte-Sul com características bem particulares. Denominamos esse padrão de relacionamento de Consenso do Pacífico (CP. Embora no curto prazo o fator China estimule o crescimento da sub-região, o CP traz implicações diferenciadas para o desenvolvimento dos países que possuem um setor industrial importante - ex. Brasil e Argentina - e aqueles que não o possuem - ex. Chile e Peru, que inclusive assinaram tratados de livre comércio com o gigante asiático. O artigo conclui com algumas considerações a respeito das conseqüências que o CP tem no processo de integração sul-americana.

  19. 78 FR 73877 - Endangered Species; Marine Mammals; Receipt of Applications for Permit

    Science.gov (United States)

    2013-12-09

    ... Felidae [includes cheetah (Acinonyx jubatus); but does not include the genus Panthera or margay (Leopardus... Crotalus willardi) Species: Bactrian wapiti (Cervus elaphus bactrianus) Amur leopard (Panthera pardus... mortem wild Amur tigers (Panthera tigris altaica) to the United Kingdom for the purpose of enhancement...

  20. Viper Plague Project

    Science.gov (United States)

    2009-07-01

    a private herpetological collection infested with ticks (Kiel et al, 2006). Viper Plague presented signs and symptoms in the reptiles that were...1. Introduction The term Viper Plague was coined for a lethal disease state seen in snakes belonging to a private herpetological collection...Rattlesnake (Crotalus atrox). This was performed because this particular species of rattlesnake was present in the original herpetological collection

  1. 50 CFR 17.95 - Critical habitat-fish and wildlife. (Continued)

    Science.gov (United States)

    2010-10-01

    ... IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) ENDANGERED AND THREATENED WILDLIFE AND PLANTS (CONTINUED...: Map follows: EC01JN91.056 New Mexican Ridge-Nosed Rattlesnake (Crotalus willardi obscurus) New Mexico... as skinks, frogs, snakes, and birds. (ii) Woodland or annual grassland plant communities...

  2. [Women's body language during the post-partum period: language understanding based on the work of Maurice Merleau-Ponty].

    Science.gov (United States)

    Machineski, Gicelle Galvan; Schneider, Jacó Fernando; Bastos, Carmen Célia Barradas Correia

    2006-09-01

    This study aimed understanding the woman's language during the post-partum period as to her perceptions of her body language. This study was based in the work of Maurice Merleau-Pont. Ten women living in Cascavel, State of Parand, Brazil, were interviewed from February to May 2005. This study allowed the understanding of the meaning that the woman gives to her existence in the post childbirth period. This study may support training of health professionals in terms of understanding how women experience the post-partum period, thereby allowing better care of these patients.

  3. Workplace health promotion: analysis of actions proposed by graduates of a training course (2012

    Directory of Open Access Journals (Sweden)

    Francielle Bertusso

    2014-12-01

    Full Text Available The incorporation of the workers knowledge in the actions of health promotion and prevention can strengthen the search for better working conditions and the protection of workers' health. This bibliographic study aimed to analyze, from the theoretical framework of occupational health, the actions proposed by graduates of a training course for health promotion for healthcare workers in 2012. Of the 221 projects analyzed, 1Enfermeira, aluna especial do Programa de Mestrado em Biociências e Saúde da Universidade Estadual do Oeste do Paraná, campus de Cascavel. E-mail: franbertusso@hotmail.com 2 Doutora em Saúde Coletiva, Professora adjunta do Curso de Enfermagem do Centro de Ciências Biológicas e da Saúde da Universidade Estadual do Oeste do Paraná, campus de Cascavel, Pr. 3 Enfermeiro da Secretaria de Estado da Saúde do Paraná - SESA. Aluno especial do Programa de Mestrado em Biociências e Saúde da Universidade Estadual do Oeste do Paraná, Campus de Cascavel, Pr. 4 Doutora em Enfermargem pela Universidade de São Paulo. Professora do Programa de Mestrado em Biociências e Saúde do Centro de Ciências Biológicas e da Saúde da Universidade Estadual do Oeste do Paraná, campus de Cascavel health workers were the priority target audience (84%, as well as managers (12%, from various regions of the country with a predominance of proposals from the southeast region (107 projects and northeast region (55 projects, and in several workplace activities. Program proposals attempted to solve or minimize three sets of problems: a related to the work environment, b labor management and c the workers' health. Examination of the proposals pointed to a working reality permeated by incidents of accidents by exposure to biological material, ionizing radiation, needlestick and sharps injuries, and injuries related to musculoskeletal overload, mental health and mental and behavioral disorder such as depression, alcoholism, stress, burnout and moral

  4. Scenedesmaceae (Chlorophyceae – Chlorococcales de um lago artificial urbano: Desmodesmus e Scenedesmus = Scenedesmaceae (Chlorophyceae – Chlorococcales of an urban artificial lake: Desmodesmus and Scenedesmus

    Directory of Open Access Journals (Sweden)

    Norma Catarina Bueno

    2007-07-01

    Full Text Available Trata-se do inventário florístico de Scenedesmaceae no Lago Municipal de Cascavel (24°82’S, 53º28´W, Paraná, Brasil. As amostras foram coletadas na região litorânea, no período de janeiro de 2002 a outubro de 2003. Foram registrados 21 táxons, sendo 13 do gênero Desmodesmus e oito pertencentes ao gênero Scenedesmus. O trabalhoapresenta descrição, chave e ilustrações para a identificação das espécies.An inventory of Scenedesmaceae was made in the Lago Municipal de Cascavel (24°82’S, 53º 28´W, Paraná, Brazil. The samples were collected in littoral zone, from January 2002 to October 2003. Twenty-one taxa were registered, 13 belong to Desmodesmus and eight to Scenedesmus genera. This paper presents description, key and illustrations to identify species.

  5. Scenedesmaceae (Chlorophyceae – Chlorococcales de um lago artificial urbano: Desmodesmus e Scenedesmus - DOI: 10.4025/actascibiolsci.v29i3.483 Scenedesmaceae (Chlorophyceae – Chlorococcales of an urban artificial lake: Desmodesmus and Scenedesmus - DOI: 10.4025/actascibiolsci.v29i3.483

    Directory of Open Access Journals (Sweden)

    Norma Catarina Bueno

    2007-12-01

    Full Text Available Trata-se do inventário florístico de Scenedesmaceae no Lago Municipal de Cascavel (24°82’S, 53º28´W, Paraná, Brasil. As amostras foram coletadas na região litorânea, no período de janeiro de 2002 a outubro de 2003. Foram registrados 21 táxons, sendo 13 do gênero Desmodesmus e oito pertencentes ao gênero Scenedesmus. O trabalho apresenta descrição, chave e ilustrações para a identificação das espécies.An inventory of Scenedesmaceae was made in the Lago Municipal de Cascavel (24°82’S, 53º 28´W, Paraná, Brazil. The samples were collected in littoral zone, from January 2002 to October 2003. Twenty-one taxa were registered, 13 belong to Desmodesmus and eight to Scenedesmus genera. This paper presents description.

  6. Incidência e aspectos clínico-laboratoriais do Lúpus eritematoso sistêmico em cidade do Sul do Brasil Incidence and clinical-laboratory aspects of systemic lupus erythematosus in a Southern brazilian city

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Kenji Nakashima

    2011-06-01

    Full Text Available INTRODUÇÃO: Estudos epidemiológicos brasileiros sobre o lúpus eritematoso sistêmico (LES são bastante escassos e os dados existentes hoje são praticamente todos de literatura internacional. OBJETIVOS: Determinar a incidência e algumas características clínicas e laboratoriais de pacientes com LES em Cascavel, Paraná - Brasil. PACIENTES E MÉTODOS: Os dados foram coletados entre agosto de 2007 e julho de 2008 em todos os serviços de saúde do município que possuíam atendimentos na especialidade de Reumatologia: um hospital universitário, um ambulatório público e três clínicas privadas da cidade. RESULTADOS: Foram identificados 14 pacientes com diagnóstico de LES, resultando em uma incidência estimada de 4,8 casos/100.000 habitantes/ano. Todos os pacientes eram do sexo feminino, com média de idade de 41,5 anos. A faixa etária com maior incidência foi a de 30 - 39 anos e 92,8% apresentaram quatro ou mais dos 11 critérios do American College of Rheumatology (ACR para o diagnóstico de LES. O tratamento farmacológico dos pacientes também foi avaliado e mostrou estar de acordo com o Consenso Brasileiro para o tratamento de LES. CONCLUSÃO: A incidência obtida em Cascavel/PR está próxima das incidências observadas em estudos internacionais.INTRODUCTION: Brazilian epidemiological studies on systemic lupus erythematosus (SLE are scarce, and currently available data originate almost entirely from international literature. OBJECTIVES: To determine the incidence and some clinical and laboratory characteristics of patients with SLE in the municipality of Cascavel, state of Paraná, Brazil. PATIENTS AND METHODS: Data were collected from August 2007 to July 2008 in all health services of Cascavel providing health care in rheumatology: a university-affiliated hospital, a public outpatient clinic, and three private clinics. RESULTS: The study identified 14 patients diagnosed with SLE, which resulted in an estimated incidence of 4

  7. Effectiveness of Metarhizium anisopliae against immature stages of Anastrepha fraterculus fruitfly (Diptera : Tephritidae Eficácia de Metarhizium anisopliae a estágios imaturos de Anastrepha fraterculus a mosca das frutas (Diptera : Tephritidae

    Directory of Open Access Journals (Sweden)

    Ricardo Henry Rodrigues Destéfano

    2005-03-01

    Full Text Available The study evaluated the effectiveness of Metarhizium anisopliae var. anisopliae (Hyphomycetes : Moniliales strain E9, isolated from the pasture spittlebug Deois flavopicta (Hemiptera : Cercopidae, against larvae, prepupae and pupae stage and emergent adults of Anastrepha fraterculus, the South American fruitfly. The bioassay was carried out simulating field conditions, on autoclaved (AS and non-autoclaved (NAS soil from typical citrus orchards in the State of São Paulo, Southeastern region of Brazil. Various concentrations of conidia were incorporated into the soil the mortality, calculated based on the percentage of adult emergence, was 86% for the highest conidia concentrations: 2.52 x 10(10 for AS and 2.52 x 10(10 for NAS. The lethal concentration (LC50, expressed as conidia concentration, was 8.44 x 10(9 conidia/g of soil (S for AS and 12.23 x 10(9 conidia/g of soil for NAS.O estudo avaliou a eficácia de Metarhizium anisopliae var. anisopliae, (Hyphomycetes : Moniliales linhagem E9, isolada da cigarrinha das pastagens Deois flavopicta (Hemiptera : Cercopidae, contra larvas, prepupas, pupas e adultos emergentes de Anastreha fraterculus, a mosca Sul Americana das frutas. Os bioensaios foram conduzidos simulando condições de campo em solo autoclavado (AS e não autoclavado (NAS de pomares típicos de citros no Estado de São Paulo, sudeste do Brasil. Várias concentrações de conídios foram incorporadas no solo. A mortalidade calculada sobre a porcentagem de adultos emergentes, foi de 86% para as concentrações mais altas de conídios: 2,52 x 10(10 para AS e 2,52 x 10(10 para NAS. A concentração letal (LC50 expressa pela concentração de conídios, foi 8,44 x 10(9 conídios/g de solo para AS e 12,2 x 10(9 conídios/g/S para NAS.

  8. Podem as novas tecnologias de informação e comunicação auxiliar na consolidação das democracias? Um estudo sobre a informatização dos órgãos legislativos na América do Sul

    Directory of Open Access Journals (Sweden)

    Sérgio Soares Braga

    2007-06-01

    Full Text Available O objetivo deste artigo é apresentar os resultados de pesquisa sobre a informatização dos órgãos legislativos da América do Sul. Procuramos refletir sobre o impacto da internet no funcionamento das instituições democráticas sul-americanas através do exame comparado da organização dos websites dos órgãos parlamentares dos diferentes países. Esse objetivo geral desdobra-se em dois objetivos específicos: a Apresentar uma proposta de mensuração do grau de informatização destes órgãos parlamentares, visando a avaliar o quanto os parlamentos destes países avançaram neste processo; b Elaborar e aplicar instrumentos teórico-metodológicos para a análise de tais dados, bem como para a avaliação do significado analítico de tais informações e sobre seu impacto no funcionamento das instituições políticas democráticas da América Latina em geral, e da América do Sul em particular, especialmente no controle dos representantes pelos cidadãos.The objective of this article is to present the results of our research about the informatization of the legislative bodies in the South America. This general objective is divided in two specific objectives: a To present a proposal of mensurement of the degree of informatization of these parliamentary agencies, evaluating how much the parliaments of these countries had advanced in this process; b To elaborate and to apply metodological instruments for the analysis of data, as well as for the evaluation of the analytical meaning of that informations, and on its impact in the functioning of the democratic institutions of Latin America in general, and the South America in particular, especially in the control of the representatives for the citizens.

  9. Amazonia boliviana: arqueología de los Llanos de Mojos Bolivian Amazonia: archaeology of the Llanos de Mojos

    Directory of Open Access Journals (Sweden)

    Horacio Adolfo Calandra

    2004-01-01

    Full Text Available A arqueologia da Amazônia boliviana ou das "Terras Baixas" compreende um imenso território que mostra, a luz da informação disponível, significativas descontinuidades espaço-temporais. A identificação nesta área de sociedades constituindo "cacicados da floresta tropical" a partir de critérios baseados em preconceitos, requer a reavaliação da pré-história regional do ponto de vista causal. A arqueologia beniana (de Llanos de Mojos é conhecida, fundamentalmente, a partir das escavações de Erland Nordenskiöld, que sem dúvida estabeleceu as bases conceituais existentes atualmente. Entre os anos de 1977 e 1981 uma missão do Museu de La Plata (Argentina, sob a direção de B. Dougherty, e em estreita colaboração com o Instituto de Arqueologia de La Paz (Bolívia e com o Amazonian Ecosystem Research (EUA, conduziu pesquisas sistemáticas considerando variados itens antropológicos e produzindo numerosas datações de radiocarbono. Estas contribuições ajudaram a esclarecer, mas não a simplificar o panorama pré-hispânico regional, tão importante na temática arqueológica sul-americana. Complementa este artigo uma exaustiva lista de bibliografias que facilita o acesso ao conhecimento sobre este grande território.The archaeology of the Bolivian Amazonia or "Low lands" comprises a large territory that shows, significant time and spatial discontinuities. The identification of societies in this area constituting "Jefaturas de la Floresta Tropical", from criteria based on preconceptions requires the reassessment of regional prehistory from the causal point of view. Benian archeology (Llanos de Mojos is fundamentally known, from the Erland Nordenskiöld excavations, which undoubtedly sets the conceptual bases existing to date. Between 1977 and 1981 a Mission of Museo de La Plata (Argentina, under the direction of B. Dougherty together with the Instituto Nacional de Arqueología of La Paz (Bolivia and the Amazonian Ecosystem

  10. Geoconservação da Ilha da Trindade: Principais Aspectos e Potencial de Uso

    Directory of Open Access Journals (Sweden)

    Gustavo Luiz Campos Pires

    2013-07-01

    Full Text Available A Ilha da Trindade situa-se próxima ao paralelo de Vitória (ES a 1.167 km da costa brasileira. É considerada como a manifestação vulcânica alcalina mais recente do Brasil (< 3,6 Ma. Ela representa o extremo leste de uma cadeia E-W de guyots (cadeia VitóriaTrindade, gerada ao longo de uma zona de fraturas e lineamentos submarinos associados à passagem de uma pluma mantélica sob a placa Sul-americana. A ilha é composta por ciclos de vulcanismo olivina-nefelinítico a fonolítico em uma série magmática alcalina que evolui segundo processos de cristalização fracionada. As unidades litoestratigráficas da ilha (Complexo de Trindade, Sequência Desejado, Fm. Morro Vermelho, Fm. Valado e Vulcão do Paredão apresentam importantes estruturas vulcânicas facilmente reconhecíveis através da geomorfologia acidentada e de grande valor paisagístico. Estruturas como necks e diques radiais (sistemas vulcânicos erodidos, extensos platôs formados por derrames de lava e/ou depósitos piroclásticos e até mesmo parte de um grande cone de cinzas, estão entre as estruturas observadas na ilha. Devido ao grande valor geológico (petrológico/petrogenético e vulcanológico, geomorfológico e histórico (que remete-se à época das grandes navegações, Trindade apresenta importância que justifica sua geoconservação e manutenção de seus geossítios além da utilização de seu patrimônio de forma sustentável. Porém, devido à localização erma e de difícil acesso, Trindade não possui potencial para o desenvolvimento de Geoturismo ou qualquer atividade que compreenda grande contingente de pessoas, pelo contrário, seu grande valor é científico e didático, sendo um importante campo escola para o ensino, não só de geologia, mas como de diferentes áreas das ciências naturais

  11. Desenvolvimento da Vigilância Epidemiológica de Fronteira no contexto da Globalização: conceitos e marcos teóricos.

    Directory of Open Access Journals (Sweden)

    Robson BRUNIERA-OLIVEIRA

    2014-12-01

    Full Text Available Objetivo. Apresentar e analisar as ações desenvolvidas para a implementação e desenvolvimento de Vigilância Epidemiológica de Fronteiras, nos Blocos Econômicos Multinacionais, particularmente na América do Sul e Brasil. Metodologia. Foi realizado um estudo de revisão narrativa incluindo artigos científicos, documentos técnicos, diretrizes, normas, manuais, pautas de reuniões ou qualquer outro documento que fosse relacionado ao desenvolvimento e implementação de ações e programas de Vigilância Epidemiológica em Fronteiras. O estudo foi baseado nos sites das instituições/organizações multinacionais e nas bases MedLine, PubMed, Scielo e Scopus. Resultados. Foram identificados a criação de órgãos e ações no intuito de implementar e desenvolver a vigilância epidemiológica de fronteiras para os seguintes blocos: União Europeia (Health Security Committee, European Center for Disease Prevention and Control, Sistema de alerta rápido e resposta, General Rapid Alert System e Rede de Monitoramento Ad Hoc; Tratado Norte-Americano de Livre Comércio (Border Infectious Disease Surveillance, “Projeto de Vigilância e Alerta Rápido de Doenças Infeciosas” ; Mercado Comum do Sul ( Reunião de Ministros da Saúde do Mercosul, Subgrupo de Trabalho 11 Saúde e Subcomissão Controle Sanitário de Portos, Aeroportos, Terminais e Passos Fronteiriços Terrestres; e União de Nações Sul-Americanas. Conclusões. Os programas e politicas desenvolvidos até o momento refletem o reconhecimento da importância do assunto por parte dos Estados Membros da OMS. Uma maior integração, com troca de informações, incluindo fluxos, frameworks, planos de preparação e o fortalecimento de áreas estratégicas como de energia e transporte deve ser incentivada intra e inter blocos.

  12. Analyses of DNA from ancient bones of a pre-Columbian Cuban woman and a child

    Directory of Open Access Journals (Sweden)

    Ricardo Lleonart

    1999-09-01

    restos no haplogrupo A, comumente encontrado em populações ameríndias. Baseado nesses resultados, nós especulamos a respeito de uma origem sul-americana para as populações pré-colombianas das Antilhas e possíveis práticas infanticidas nessas populações. Este constitui o primeiro relato de análise de DNA em populações cubanas pré-colombianas antigas.

  13. Atividade antiinflamatória do extrato aquoso de Arrabidaea chica (Humb. & Bonpl. B. Verl. sobre o edema induzido por venenos de serpentes amazônicas Anti-inflammatory activity of the aqueous extract of Arrabidaea chica (Humb. & Bonpl. B. Verl. on the self-induced inflammatory process from venoms amazonians snakes

    Directory of Open Access Journals (Sweden)

    Denys Paixão Costa de Oliveira

    2009-06-01

    Full Text Available O estudo investigou o efeito antiinflamatório do extrato aquoso da Arrabidaea chica (Humb. & Bonpl. B. Verl., Bignoniaceae, popularmente conhecida como "crajiru", sobre o edema induzido por venenos de serpentes amazônicas dos gêneros Brothrops e Crotalus, em camundongos albinos, por via oral, intraperitoneal e subcutânea. O efeito anti-edematogênico foi avaliado pela medição do diâmetro dos coxins das patas posteriores, sendo medidos as 1, 3, 6, 12 e 24 horas, para B. atrox e 1, 3 e 6 horas para Crotalus durissus ruruima, e também avaliado por histopatologia. O estudo mostrou que o efeito inibitório do extrato aquoso para o gênero Bothrops, pelas vias subcutânea e intraperitoneal (12 horas foi de 55,87% e 65,70%, respectivamente. Para o gênero Crotalus o efeito inibitório do extrato pela via subcutânea após 3 horas foi de 33,55% e após 6 horas de 79,81%. Pela via intraperitoneal após 3 horas foi de 48,02% e após 6 horas de 92,52%. Na análise histopatológica, o infiltrado de granulócitos e a miocitólise foram os efeitos inflamatórios inibidos mais significativamente. Os resultados sugerem a presença no extrato aquoso de A. chica de substâncias com atividade inibitória sobre os efeitos inflamatórios dos venenos das serpentes Bothrops atrox e Crotalus durissus ruruima.The study investigated the anti-inflammatory effects of the aqueous extract (EAq, the Arrabidaea chica (Humb. & Bonpl. B. Verl., Bignoniaceae, known as the "crajiru", tested on the paw oedema induced in inflammatory process by venoms Amazon snakes from the Bothrops and Crotalus species, on albino mice. This process was done by an oral, intraperitoneal and subcutaneous way. The cushions thickness was measured at time breaks of 1, 3, 6, 12 and 24 hours, Bothrops genus and 1, 3 and 6 hours, Crotalus genus, and a histopathologic analysis was made. The Bothrops genus, was used as an inhibitory effect for the subcutaneous and intraperitoneal way (12 hours, and it

  14. A percepção dos profissionais sobre a assistência ao parto domiciliar planejado La percepción de los profesionales acerca de la atención al parto domiciliario planeado The perception of professionals regarding planned home birth

    Directory of Open Access Journals (Sweden)

    Tatianne Cavalcanti Frank

    2013-03-01

    Full Text Available Estudo qualitativo com objetivo de compreender a percepção dos profissionais no acompanhamento do parto domiciliar planejado. Foram entrevistados oito profissionais que atuaram em partos domiciliares, em Cascavel/PR. A análise revelou que o domicílio, enquanto local de assistência, possibilita o protagonismo da mulher e da família pela tranquilidade, calma e autonomia. O ambiente é seguro quando se segue requisitos como baixo risco gestacional, avaliação adequada, no decorrer da evolução do parto, presença de materiais adequados, rede transdisciplinar e local pré-definido para encaminhamentos. Os profissionais apontam, ainda, como fundamental, a participação familiar no processo. Conclui-se que o parto domiciliar é uma excelente estratégia para transformar e melhorar a qualidade da atenção obstétrica.Estudio cualitativo con el objetivo de comprender la percepción de los profesionales en el acompañamiento del parto domiciliario planeado. Fueron entrevistados ocho profesionales que trabajaron en los partos domiciliarios en Cascavel/PR. El análisis reveló que el domicilio como un lugar de atención posibilita el protagonismo de la mujer y de la familia por la tranquilidad, calma y autonomía. El ambiente es seguro cuando se siguen los requisitos tales como bajo riesgo gestacional, evaluación adecuada de la evolución del parto, presencia de materiales adecuados, red transdisciplinaria y local predefinido para encaminamientos. Los profesionales apuntan también como fundamental la participación familiar en el proceso. Se concluyó que el parto domiciliario es una excelente estrategia para transformar y mejorar la calidad de la atención obstétrica.This is a qualitative study aimed to understand the perception of professionals regarding planned home birth. Eight professionals who took part in home births in Cascavel/PR were interviewed. The analysis revealed that home, as the care place, allows more prominence to women and

  15. Acompanhamento de enfermagem em saúde pública, assessoramento pedagógico e agrícola numa comunidade de assentados sem-terra na zona rural: relato de experiência Acompañamiento de enfermería en salud pública, asesoramiento pedagógico y agrícola en una comunidad de asentados "sin tierra" en la zona rural: relato de experiencia Public health nursing, pedagogical and agricultural ad vice in a rural community of settled landless people: an experience

    Directory of Open Access Journals (Sweden)

    Jacó Fernando Schneider

    1995-01-01

    Full Text Available O estudo relata uma experiência no acompanhamento de Enfermagem, assessoramento pedagógico e agrícola desenvolvido em uma comunidade de assentados sem-terra na zona rural da cidade de Cascavel, estado do Paraná, que teve como premissa básica a integração docente/discente, oferecendo oportunidade de socialização do saber, integração teoria e prática e contato direto com a comunidade. Aponta para a necessidade da enfermagem compreender melhor este tipo de realidade buscando, através da observação e vivência, uma visão crítica com possibilidades de intervenção.El estudio relata una experiencia en el acompañamiento de enfermería, asesoramiento pedagógico y agrícola desarrollado en una comunidad de asentados "sin tierra" en la zona rural perteneciente al municipio de Cascavel, estado del Paraná, que tuvo como principio básico la integración docente/alumno, ofreciendo oportunidad de socialización del saber, integración teoría y práctica y contacto directo con la comunidad. Señala la necessidad de la enfermería comprender mejor este tipo de realidad, buscando por medio de la observación y vivencia, una visión crítica con posibilidades de intervención.This study reports an experience in nursing accompaniment, pedagogical and agricultural advice in a community of settled people without land in the countryside of Cascavel, state of Paraná. The basic premise was student/teacher integration offering the opportunity of knowledge socialization, integration of theory and practice and direct contact with the community. It points out that nursing needs a better understanding of this type of reality seeking, by observation and living, a critical vision with the possibility of intervention.

  16. QTL mapping for protein content in soybean cultivated in two tropical environments Mapeamento de QTL quanto ao conteúdo de proteína em soja cultivada em dois ambientes tropicais

    Directory of Open Access Journals (Sweden)

    Taís Cristina Bastos Soares

    2008-11-01

    Full Text Available The objectives of this study were to detect quantitative trait loci (QTL for protein content in soybean grown in two distinct tropical environments and to build a genetic map for protein content. One hundred eighteen soybean recombinant inbred lines (RIL, obtained from a cross between cultivars BARC 8 and Garimpo, were used. The RIL were cultivated in two distinct Brazilian tropical environments: Cascavel county, in Paraná, and Viçosa county, in Minas Gerais (24º57'S, 53º27'W and 20º45'S, 42º52'W, respectively. Sixty-six SSR primer pairs and 65 RAPD primers were polymorphic and segregated at a 1:1 proportion. Thirty poorly saturated linkage groups were obtained, with 90 markers and 41 nonlinked markers. For the lines cultivated in Cascavel, three QTL were mapped in C2, E and N linkage groups, which explained 14.37, 10.31 and 7.34% of the phenotypic variation of protein content, respectively. For the lines cultivated in Viçosa, two QTL were mapped in linkage groups G and #1, which explained 9.51 and 7.34% of the phenotypic variation of protein content. Based on the mean of the two environments, two QTL were identified: one in the linkage group E (9.90% and other in the group L (7.11%. In order for future studies to consistently detect QTL effects of different environments, genotypes with greater stability should be used.Os objetivos deste trabalho foram detectar QTL relativos ao conteúdo de proteína, em soja cultivada em dois ambientes tropicais divergentes, e construir um mapa genético para o conteúdo de proteína em genótipos adaptados a condições tropicais. Foram usadas 118 linhagens recombinantes endogâmicas de soja, obtidas do cruzamento entre as cultivares BARC 8 e Garimpo. A população de linhagens recombinantes endogâmicas foi cultivada em dois ambientes contrastantes: Cascavel, PR, e Viçosa, MG (24º57'S, 53º27'W; e 20º45'S, 42º52'W, respectivamente. Sessenta e seis pares de iniciadores SSR e 65 iniciadores RAPD

  17. The Defense of Socio-Educational Internment: Feature of the Hygienist Principles

    Directory of Open Access Journals (Sweden)

    Juliana Biazze Feitosa

    2014-04-01

    Full Text Available Youth violence and interventions to affront it are the focus of our scientific research. The aim of this study is evaluate the existence of the aspects contained in the justifications of hygienism judgments of teenagers who meet admission youth work. In this sense, we 21 sentences that determined the internment and its supporting materials which makes up the judicial process of teenagers that have entered the year 2010 in the Center of Socio-education II in Cascavel Paraná State. The results led us to conclude that nowadays, notwithstanding the Children and Adolescent, we are still, by judicial determination, removing the Teen Delinquency situation and closed the judicial term returning it to the same context from which it was withdrawn. Enshrined due differences in shape and time note this routing aspects of the ideology of mental hygiene and the minor doctrine.

  18. Enteroparasitos em materiais fecal e subungueal de manipuladores de alimentos, Estado do Paraná, Brasil - DOI: 10.4025/actascihealthsci.v31i2.4935 Enteroparasites in fecal and subungual matter from food handlers, Parana State, Brazil- DOI: 10.4025/actascihealthsci.v31i2.4935

    Directory of Open Access Journals (Sweden)

    Dina Lúcia Moraes Falavigna

    2009-09-01

    Full Text Available Este estudo indica a presença de enteroparasitos em material fecal e subungueal de 343 manipuladores de alimentos de Cascavel, Estado do Paraná, Brasil, de ambos os gêneros e com idade entre 14 e 75 anos. O material fecal foi analisado pelos métodos de Lutz, Ritchie modificado e Ziehl-Neelsen modificado e o subungueal, pelo Ritchie. O material fecal foi positivo para 131 (38,2% manipuladores. As espécies mais prevalentes foram Endolimax nana (67,9%, Entamoeba coli (35,9%, Blastocystis sp. (28,2%, Entamoeba histolytica/díspar (10,1% e Giardia duodenalis (8,4%, e E. nana foi diferente (p = 0,00 em relação às demais. A infecção por protozoários foi maior do que por helmintos (p = 0,00. Foi verificada associação entre a positividade para algum parasito e o gênero masculino e a categoria ocupacional (p ≤ 0,05. O material subungueal foi positivo em 17 indivíduos (5,0%, com encontro de E. nana (2,9%, E. coli (1,2%, G. duodenalis (0,3% e a associação de E. nana e E. coli (0,6%, não havendo convergência com o material fecal (p = 0,00. A ocorrência de enteroparasitos nos manipuladores de alimentos de Cascavel, Estado do Paraná é alta, indicando condições higiênicas inadequadas e a necessidade de medidas educativas rígidas, para se evitar que agentes patogênicos para o homem sejam propagados para os alimentos pela manipulação.This study shows the presence de enteroparasites in fecal and subungual material of 343 food handlers in the city of Cascavel, Paraná State, Brazil, from both genders and ages between 14 and 75 years. Fecal samples were analyzed using Lutz, modified Ritchie and Ziehl-Neelsen techniques, and subungual material was analyzed using the Ritchie method. Fecal samples were positive in 131 (38.2% handlers. Species with highest prevalence were Endolimax nana (67.9%, Entamoeba coli (35.9%, Blastocystis sp. (28.2%, Entamoeba histolytica/dispar (10.1% and Giardia duodenalis (8.4%, with E. nana being significantly

  19. Deficiência de ferro, prevalência de anemia e fatores associados em crianças de creches públicas do oeste do Paraná, Brasil Iron deficiency and prevalence of anemia and associated factors in children attending public daycare centers in western Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Valdete Carreira Rodrigues

    2011-06-01

    Full Text Available OBJETIVO: Avaliar o estado nutricional de ferro, a prevalência de anemia e fatores associados, em crianças de 6 a 24 meses frequentadoras de creche pública em Cascavel, Região Oeste do Paraná, Brasil. MÉTODOS: O estudo transversal foi realizado com amostra aleatória de 256 crianças. A coleta de dados (questionário, medidas antropométricas e amostras de sangue ocorreu de julho a setembro de 2007. A deficiência de ferro foi avaliada em termos de transferrina, hemoglobina, volume corpuscular médio, ferro sérico e eosinófilos. Na análise estatística dos dados foram obtidas as odds ratio bruta e ajustada (regressão logística, bem como os respectivos níveis de significância (p-valor. Para identificar diferenças significativas entre as medidas quantitativas, adotou-se a Análise de Variância e o teste de comparação múltipla de Tukey. RESULTADOS: A prevalência da anemia foi de 29,7%, sendo que 77,3% das amostras apresentaram baixa concentração de ferro. A antropometria não apontou deficiência de macronutrientes, porém mostrou obesidade acima dos índices médios. Os fatores associados à anemia e à deficiência de ferro foram: doenças frequentes na família (OR=10,02, condições de moradia (OR=5,05, tempo de creche (OR=3,05, número de moradores na residência (OR=2,83 e falta de saneamento (OR=2,20. CONCLUSÃO: A prevalência de anemia e a elevada deficiência de ferro detectada evidenciam um grave problema de saúde pública entre os pré-escolares do município de Cascavel, Paraná. Apesar da amplitude do problema, a anemia não está sendo reconhecida, prevenida e tratada adequadamente. Neste estudo são sugeridas algumas possíveis intervenções.OBJECTIVE: This study assessed the iron levels and prevalence of anemia and associated factors in children aged 6 to 24 months attending public daycare centers in Cascavel, Western Paraná, Brazil. METHODS: This cross-sectional study included 256 randomly sampled children

  20. THE ISSUE OF EDUCATION IN MARX: SOME NOTES

    Directory of Open Access Journals (Sweden)

    João Carlos da Silva

    2011-02-01

    Full Text Available : Article as a result of readability and reflections carried out in the study group on the works of Marx and Engels, within the group of search HISTEDOPR - GT, Cascavel, PR. The question educational not received a specific treatment in the work of Marx, even if many seek in their formulations, theoreticalmethodological principles for the process of teaching-learning. Its educational thinking must be observed in the whole production marxist. This text to understand, in the light of historical change, the question educational, based on some of his writings. Their conception of education is immersed in horizon of socioeconomic relations, central object of discussion. We will see that education, in Marx, presents a politicalideological perspective, even before the determinant economic. Understand the analysis of Marx about the educative phenomenon does without to observe its way to understand the society.

  1. Aspectos morfológicos de frutos e sementes e caracterização citogenética de Crotalaria lanceolata E. Mey. (Papilionoideae - Fabaceae Morphological aspects of fruits and seeds and cytogenetic characterization of Crotalaria lanceolata E. Mey. (Papilionoideae - Fabaceae

    Directory of Open Access Journals (Sweden)

    Débora Aparecida Verde de Andrade

    2008-09-01

    Full Text Available Frutos, sementes e plântulas de Crotalaria lanceolata, conhecida popularmente como guizo-de-cascavel, chocalho-de-cobra, xique-xique ou feijão-de-guizo, planta tóxica infestante que ocorre no Estado de São Paulo, foram estudadas morfologicamente e citogeneticamente. Os frutos são secos, deiscentes, polispérmicos e do tipo legume. As sementes são reniformes e o embrião é constituído de eixo embrionário e dois cotilédones. A testa pode apresentar variadas tonalidades de castanhos. A germinação é epígea e fanerocotiledonar. A espécie apresenta número cromossômico diplóide 2n = 16 com formulação cariotípica 12M + 4SM e comprimento cromossômico médio geral de 3,340 ± 0,689.Crotalaria lanceolata, known popularly as "guizo-de-cascavel", "xique-xique" or "feijão-de-guizo", is a toxic weed that occurs in the state of Sao Paulo. Fruits, seeds and seedlings were studied morphologically and cytogenetically. The fruits are dry, dehiscent, polyspermic legumes. The seeds are kidney shaped and the embryo consists of an embryonic axis and two cotyledons. The seed tegument comes in various chestnut tones. Germination is phanerocotylar and epigeal. The species has a mitotic chromosome number 2n = 16 with karyotype formula 12M + 4SM; overall mean chromosome length is 3.340 ± 0.689.

  2. Environmental Assessment for a Commercial Vehicle Inspection Point at the Downs Road Gate

    Science.gov (United States)

    2013-02-01

    for Florida black bear (Ursus americanus floridanus), other mammals , amphibians and reptiles such as diamondback rattlesnakes (Crotalus adamanteus...East Gate and the Main Gate provides habitat for perching and song birds and small mammals accustomed to urban environments. 3.10 Listed Species...Road Suite A Pensacola, FL 32514 850-332-7976 ext. 203 or Toll Free 1-800-226-8914 ext. 203 Fax: 850-637-1923   Staff to the Florida‐ Alabama  TPO

  3. Contribuição à biologia de serpentes da Bahia, Brasil: I. vivíparas Contribution to reproductive biology of snakes in Bahia, Brazil: I. viviparous

    Directory of Open Access Journals (Sweden)

    Rejane Maria Lira-da-Silva

    1994-01-01

    Full Text Available Great part of lhe avaiable data about snakes reprodution refers to species coming from subtropical and temperate regions. In Brazil, the data is rather rare and can be found in various works where information is restricted. Results from studies developed with five viviparous snakes - Crotalus durissus cascavella (Wagler, 1824. Bothrops erythromelas(Amaral, 1923, B. leucurus (Wagler, 1824, Helicops leopardinus (Schlegel, 1873 and Thamnodynastes strigilis (Thiinberg, 1787 - which come from the Northeast of Brazil (Bahia are described. Data about pregnancy and birth, number, sex ratio, length and weight of neonates is given and discussed.

  4. Structural studies of the oligomeric formation crotoxin and their complexes; Estudos estruturais da formacao oligomerica da crotoxina e seus complexos

    Energy Technology Data Exchange (ETDEWEB)

    Morelli, Humberto A.S.; Fernandes, Carlos A.H.; Magro, Angelo J.; Fontes, Marcos R.M.; Fernandez, Roberto M., E-mail: rmorato@ibb.unesp.br [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Inst. de Biociencias. Dept. de Fisica e Biofisica; Bicev, Renata N.; Oliveira, Cristiano L.P. de [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Inst. de Fisica. Dept. de Fisica Experimental; Barioni, Marina B.; Ito, Amando S. [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Fac. de Filosofia, Ciencias e Letras. Dept. de Fisica

    2013-08-15

    The crotoxin is a phospholipase A{sub 2} that is the mainly responsible for the neurotoxic effect of the snakes' venom of the species Crotalus durissus, popularly known as rattlesnakes. In the crotoxin there are two subunits: an acidic, non-toxic and devoid of enzymatic action, (called crotoxin A or CA); and a basic, toxic with enzymatic activity (called crotoxin B or CB). The main objective of this work is to study the CA, CB and the complex CA + CB using the Small-Angle X-ray Scattering (SAXS), Optical absorption spectrometry and static fluorescence techniques. (author)

  5. Antivenenos ofídicos: comparación del desempeño de dos métodos de obtención

    OpenAIRE

    2015-01-01

    El envenenamiento por serpientes venenosas es frecuente en algunas zonas de Argentina, particularmente en las provincias del noreste y noroeste. Según datos del Programa Nacional de Ofidismo, el número de mordeduras es de alrededor de 1.200 anuales; son producidas casi totalmente por víboras del género Bothrops (yarará), en mucho menor número por Crotalus (cascabel) y en una cantidad todavía mucho más escasa por serpientes Micrurus (coral). Fil: Ávila, Lucía. Ministerio de Salud de la Naci...

  6. Phylogenetic correlograms and the evolution of body size in South American owls (Strigiformes

    Directory of Open Access Journals (Sweden)

    José Alexandre Felizola Diniz-Filho

    2000-06-01

    ção interespecífica no tamanho do corpo de 19 espécies de corujas (Strigiformes sul-americanas através de correlogramas filogenéticos, construídos utilizando índices I de Moran em quatro classes de distância. A filogenia entre as espécies foi definida com base em dados de hibridização de DNA. O correlograma observado foi então comparado a 500 correlogramas obtidos através de simulações de evolução por movimento Browniano e pelo processo O-U, sobre essa mesma filogenia. Esses correlogramas foram comparados entre si utilizando análises de variância (ANOVA e MANOVA e através das correlações entre os índices I de Moran e as classes de distância filogenética. O correlograma observado indica a existência de um gradiente filogenético de variação até cerca de 45 milhões de anos, quando os índices se estabilizam, e é similar aos correlogramas obtidos através do processo O-U, considerando tanto a correlação do gradiente quanto a sua alocação aos dois grupos de processos através de análise discriminante. Esse padrão é esperado, considerando a importância do tamanho do corpo e sua correlação com diversos caracteres ecológicos e de história de vida, que produzem muitas restrições que podem de fato ser modeladas por um processo O-U expressando seleção estabilizadora.

  7. Diversidade alfa e beta no cerrado sensu strictu da Chapada Pratinha, Brasil Alfa and Beta diversity in the cerrado sensu stricto

    Directory of Open Access Journals (Sweden)

    Maria Cristina Felfili

    2001-08-01

    Full Text Available O bioma cerrado possui uma das mais ricas floras dentre as savanas mundiais com mais de 6000 espécies, abrange uma vasta extensão territorial, contém as três maiores bacias hidrográficas sul americanas, e se destaca pela elevada biodiversidade. O objetivo deste trabalho, que faz parte do Projeto Biogeografia do Bioma Cerrado, foi analisar as diversidades alfa e beta em comunidades de cerrado sensu stricto. Foram amostrados o Parque Nacional de Brasília, a Estação Ecológica de Águas Emendadas, a Area de Proteção Ambiental (APA Gama-Cabeça de Veado, Silvânia-GO, Paracatu-MG e Patrocínio-MG. A amostragem foi aleatória com 10 parcelas de 20x50m em cada área de estudo. Foram incluídas plantas lenhosas, exceto lianas, que tivessem no mínimo 5cm de diâmetro. Foi calculada a diversidade alfa pelo teste de Shannon & Wienner e o de Simpson. A diversidade beta foi calculada pelo índice de Whittaker que mede a mudança ou taxa de substituição na composição de espécies de um local para outro. Foi efetuada a curva espécie-área para as 60 parcelas amostradas e efetuada a classificação por TWINSPAN. O cerrado sensu stricto da Chapada Pratinha pode ser diferenciado em duas zonas fitogeográficas: Distrito Federal-Silvânia e Paracatu-Patrocínio. Estas coincidem com um zoneamento por sistemas de terra que classifica a primeira zona como terras altas em contraposição à segunda.The cerrado flora is one of the richest among the world's savannas with more than 6000 species. The cerrado covers a large territory, contains the three most important hydrographycal basins in South America and a high diversity. The objective of this work, that is part of the project Biogeography of the Cerrado Biome, was to analyse alfa and beta diversity in the cerrado sensu stricto. The sampled places were: Brasília National Park -- DF, Águas Emendadas Ecological Station -- DF, Environmentally protected Area of Gama-Cabeça de Veado -- DF, Silv

  8. Evolução e gênese de um Plintossolo Pétrico concrecionário êutrico argissólico no município de Ouro Verde de Goiás Evolution and genesis of a petroferric eufrophic alfisol in Ouro Verde de Goiás, Brazil

    Directory of Open Access Journals (Sweden)

    Hilton Lenzi Moreira

    2008-08-01

    Full Text Available Solos concrecionários (Plintossolo Pétrico concrecionário nas Regiões Central e Norte do Brasil são muito comuns, ocorrendo em áreas baixas e nas bordas das chapadas, constituindo geralmente solos distróficos,pobres em nutrientes. A ocorrência de solo concrecionário eutrófico no município de Ouro Verde de Goiás, em área de borda de chapada motivou esta pesquisa, desenvolvida com a finalidade de verificar a relação eutrofia/solo/material litológico subjacente, provável fonte das bases trocáveis. As relações pedológicas e geoquímicas do solo com as concreções e a rocha de origem foram pesquisadas por meio de determinações da constituição química total dos elementos maiores, utilizando ICP-AES (espectrometria de emissão atômica por plasma acoplado indutivamente, análise mineralógica da fração argila por DRX, análise mineralógica da fração areia, além da caracterização analítica de rotina em pedologia. Os resultados analíticos de amostras de rocha, concreções ferruginosas e TFSA, as relações moleculares Ki e Kr e bases/R2O3 e a relação Zr/Ti são abordados e discutidos. Ocorrências mais significativas quanto a alguns elementos móveis são creditadas ao conteúdo elevado de matéria orgânica no horizonte superficial do solo, ao acúmulo residual de quartzo e à contribuição de material externo nos horizontes superiores . A relação solo/material litológico subjacente indica para o perfil de origem a partir de um metatonalito, atribuindo-se ao material petroplíntico presente na porção superior do solo, origem relacionada à desagregação e arraste de materiais relacionados à Superfície de Aplanamento Sul-Americana.The occurrence of concretionary soils in the Central and Northern Brazil is very common. They are found in low areas and on plateau edges, usually constituting dystrophic soils,poor in nutrients. The finding of an eutrophicc concretionary soil in the edge of a plateau in Ouro

  9. De homens, árvores e sapos: forma, espaço e tempo em Tapirapé

    Directory of Open Access Journals (Sweden)

    Yonne Leite

    1998-10-01

    Full Text Available Em várias línguas não-indo-européias, categorias gramaticais como nome, verbo, numerais etc. são classificadas segundo propriedades ou características físicas de seus referentes. Apresenta-se aqui a classificação que expressa, em tapirapé (família tupi-guarani, Brasil, as propriedades de forma ("chata", "redonda", "alta" que se aliam, com restrições combinatórias, à posição ("deitado", "sentado", "de pé" do objeto referenciado. A comparação feita com sistemas de classificação de línguas sul-americanas indica que, apesar da diversidade categorial aparente, é possível detectar não só um número reduzido de componentes semânticos geométricos sempre atuantes, mas também coincidências na inclusão ou exclusão dos membros nas diferentes classes. O exame, mesmo superficial, parece contrariar os pressupostos das versões, moderada ou extremada, do relativismo lingüístico ou de uma infinidade de recortes de um mundo que lá está. No caso tapirapé, o que está em jogo não é a expressão culturalmente filtrada de um mundo preexistente, mas antes a apreensão de uma realidade que se constrói a partir da perspectiva que o falante tem de um evento, de um fato ou de uma existência, que se consubstancia através de uma geometria extremamente simples, mas altamente rentável, em que forma, espaço, tempo/aspecto são interdependentes.In various non-Indo-European languages, grammatical categories such as name, verb and number are classified according to the physical properties or characteristics of their referents. Presenting material on the Tapirapé language (Tupi-Guarani family, Brazil, this paper focuses on a linguistic classification which expresses formal properties ("flat", "round", "tall" in alliance with the position of the referred object ("lying", "seated", "standing" within a system subject to combinatory restrictions. Comparison with classificatory systems of other South American Indian languages reveals that

  10. Ação de venenos de serpentes brasileiras sôbre a alexina ou complemento

    Directory of Open Access Journals (Sweden)

    F. da Rocha Lagôa

    1947-06-01

    Full Text Available O autor estudou a ação inativante, sôbre o complemento de cobaia, alguns venenos de serpentes brasileiras pertencentes às famílias dos Elapideos e Crotalideos. Da primeira, foi utilizado veneno de Micrurus frontalis, da segunda, foram usados venenos de espécies pertencentes aos gêneros Crotalus (C. terrificus e Bothrops (B. atrox, B. neuwiedii, B. jararaca, B. ja-raracussú, B. cotiara e B. alternata. O venenos de M. frontalis e C. terri¬ficus se revelaram incapazes de inativar o complemento, ao passo que os diversos de Bothrops empregados se mostraram altamente inativantes, destruindo sempre o 4.° componente do complemento (C4, fração idêntica à afetada pela ação da amônea.The author shows in this paper the results of the inactivation of com¬plement or alexin by some Brazilian snakes venoms of the Elapideae and Cro¬talideae families. The venom of Micrurus frontalis (Elapideae family and of Crotalus terrificus (Crotalideae family did not destroy the complement; but the venoms of Bothrops generus (B. atrox, B. neuwiedii, B. jararaca, B. jararacussú, B. cotiara e B. alternata, (Crotalideae family are highly active in the inactivation complement, always destroing the fourth component, whichis exactly the same component that is detroyed by ammonia.

  11. Prevalence of Hepatozoon spp. (Apicomplexa, Hepatozoidae among recently captured Brazilian snakes

    Directory of Open Access Journals (Sweden)

    O'Dwyer L.H.

    2003-01-01

    Full Text Available The goal of this study was to determine the prevalence of Hepatozoon spp. infection in recently captured snakes from Botucatu, São Paulo State, Brazil. Blood was collected from all snakes by ventral tail venipuncture. Blood smears were air dried, fixed with methanol, and stained with 10% Giemsa solution. The slides were microscopically examined for detection of hemoparasites by light microscopy at 250x magnification. A total of 238 snakes from 23 species were examined, of which 135 (56.7% were venomous and 103 (43.3% non-venomous snakes. The more numerous venomous species sampled were Crotalus durissus terrificus (n=108 and Bothrops jararaca (n=17 and non-venomous snakes were Oxyrhopus guibei (n=35, Boa constrictor amarali (n=18, and Waglerophis merremi (n=13. Hepatozoon spp. infection was detected in 39 (16.4% snakes. The prevalence in venomous and non-venomous snakes was 20.0% and 11.7%, respectively. The highest prevalences observed were 38.9% for Boa constrictor amarali, 35.3% for Bothrops jararaca, and 19.4% for Crotalus durissus terrificus.

  12. Crotamine and crotoxin interact with tumor cells and trigger cell death

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Marcella Araugio; Pujatti, Priscilla Brunelli; Santos, Raquel Gouvea dos [Centro de Desenvolvimento da Tecnologia Nuclear CDTN/CNEN-MG, Belo Horizonte, MG (Brazil)]. E-mails: maso@cdtn.br; santosr@cdtn.br; Dias, Consuelo Latorre Fortes [Fundacao Ezequiel Dias FUNED, Belo Horizonte, MG (Brazil); Chavez Olortegui, Carlos Delfin [Universidade Federal de Minas Gerais UFMG, Belo Horizonte, MG (Brazil). Inst. de Ciencias Biologicas; Santos, Wagner Gouvea dos [Medical College of Virginia, Richmond, VA (United States). Neurosurgery Dept.

    2007-07-01

    Crotoxin (Crtx) and Crotamine (Crota) are polypeptides isolated from Crotalus durissus terrificus snake venom (CV). Previous reports have been shown therapeutic effects of Crotalus durissus terrificus venom and Crtx on skin, breast and lung tumours, although, the mechanisms of this antitumoral effect are still unknown. The aim of this work was to investigate the antitumoral effect of Crtx and Crota on brain tumours cells (GH3 and RT2) in vitro and their capacity of interaction with these tumour cells membranes. Cell survival after Crtx and Crota treatment was evaluated by MTT assay in different times post-treatment and apoptosis was evaluated by DAPI staining. In order to evaluate the specific interaction of Crtx and Crota, these polypeptides were radiolabelled, using {sup 125}I as radiotracer and binding assays were performed. The results were compared with the binding in nontumoral brain tissue. Crtx and Crota induced apoptosis on both tumour cells lineages but, Crota was more powerful than Crtx 90% and 20% cell death for RT2 cells; 80% and 20% cell death for GH3 cells, respectively). Both {sup 125}I-Crtx and {sup 125}I-Crota bound specifically in glioblastoma membranes. Nonetheless, CV polypeptides recognised glioblastoma cells with higher specificity than normal brain tissue. These results suggest that the Crtx and Crota interactions with the plasmatic membrane of tumour cells may be the first step of the cascade of signalling that trigger their antitumoral effect. (author)

  13. Structural aspects of crotalic venom proteins modified by ionizing radiation; Aspectos estruturais de proteinas do veneno crotalico modificadas por radiacao ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto de

    2010-07-01

    Snake bites are a serious public health problem, especially in subtropical countries. In Brazil, the Ministry of Health notified around 26 000 accidents in 2008. The genus Crotalus (rattlesnakes) accounts for approximately 7% of the total, with a high mortality rate of 72% when untreated with the specific serum, the only effective treatment in case of snake bites. In Brazil, the serum is produced in horses which, despite the large size, have a reduced lifespan due to the high toxicity of the antigen. Ionizing radiation has proven to be an excellent tool for reducing the toxicity of venoms and isolated toxins, resulting in better immunogens for serum production, and contributing to the welfare of serum producing animals. Since the action of gamma radiation on venoms and toxins has not been yet fully clarified from the structural point of view, we proposed in this paper, to characterize two toxins of the species Crotalus durissus terrificus: crotoxin and crotamine. After isolation of the toxins of interest by chromatographic techniques, they were subjected to structural analysis with the application of the following methods: Fluorescence, Circular Dichroism, Differential Calorimetry and Infrared Spectroscopy. These tests showed that both crotamine as crotoxin when subjected to gamma radiation, showed changes in their structural conformation compared with the samples in the native state. Such changes probably occur in the secondary and tertiary structure and may explain the changes on the biological activity of these toxins. (author)

  14. Estudo da morbidade em uma população idosa Estudio de la morbilidad en la población de ancianos Morbidity study in an elder population

    Directory of Open Access Journals (Sweden)

    Elizabeth Aparecida de Souza

    2011-06-01

    Full Text Available Este estudo objetivou identificar os agravos que mais acometeram os idosos no período de 1998 a 2005 e a capacidade funcional dos residentes na área de abrangência de quatro unidades básicas de saúde de diferentes regiões, no Município de Cascavel, Paraná, em 2007. Os dados de morbidade foram pesquisados no Sistema de Informações Hospitalar do Sistema Único de Saúde, e foram realizadas entrevistas com 141 idosos. No período estudado, a morbidade pouco variou: as doenças do aparelho circulatório foram responsáveis pelo maior número de internamentos. A média geral de doenças referidas pelos idosos foi de 2, com destaque para as cardiovasculares. Dentre os entrevistados, 136 (96,5% afirmaram realizar todas as atividades cotidianas, sem o auxílio de outras pessoas. O estudo mostra que é necessário implantar serviços voltados para as doenças crônicas, com foco na manutenção da máxima capacidade funcional pelo maior tempo possível, valorizando a autonomia e a autodeterminação dos idosos.El objetivo del estudio fue identificar las enfermedades que más afectaron a los ancianos en el período entre 1998 y 2005 y la capacidad funcional de los residentes en el área de cobertura de cuatro unidades básicas de salud de diferentes regiones en la cuidad de Cascavel, Paraná, en 2007. Los datos de morbilidad fueron investigados en el Sistema de Información Hospitalario del Sistema Único de Salud, e se realizaron encuestas con 141 ancianos. En el periodo estudiado, la morbilidad ha variado poco, las enfermedades del sistema circulatorio fueron responsables por el mayor número de internamientos. El promedio general de las enfermedades referidas por los mayores fue de 2, con relieve para las cardiovasculares. Entre los encuestados, 136 (o sea el 96,5% afirmaron realizar todas las actividades cotidianas sin la ayuda de los demás. El estudio muestra la necesidad de implementar servicios dirigidos a las enfermedades crónicas, con

  15. Criança hospitalizada: percepção das mães sobre o vínculo afetivo criança-família Niño hospitalizado: el vinculo afectivo niño-família Hospitalized children: mothers' perception about the affective child-family link

    Directory of Open Access Journals (Sweden)

    Beatriz Rosana Gonçalves de Oliveira

    1999-12-01

    Full Text Available Este trabalho objetivou verificar a percepção da mãe que tem seu filho doente hospitalizado, sobre a importância de sua permanência durante o período de internação para manutenção do vínculo afetivo criança-família. Os dados empíricos foram coletados com mães de crianças hospitalizadas, que não estavam acompanhando a internação do seu filho, num hospital público em Cascavel - Paraná, utilizando-se entrevista semi-estruturada. A análise dos dados demonstrou que as mães reconhecem a importância em permanecer com seu filho num período de estresse como o da hospitalização, porém, os fatores externos que influenciam seu cotidiano, muitas vezes não permitem que exerçam sua vontade de ficar com seus filhos.Este trabajo tiene como objetivo verificar la percepción de la madre que tiene su hijo enfermo hospitalizado, sobre la importancia de su permanencia durante el periodo de internación para la manutención del vinculo afectivo del niño-familia. Los dados empíricos fueron colectados con madres de niños hospitalizados, que no estaban acompañando la internación de su hijo, en un hospital publico en Cascavel ¾ Paraná, utilizase una entrevista semi-estructurada. El análisis de los datos demostró que las madres reconocen la importancia en permanecer con su hijo en un periodo de estrés como de la hospitalización, sin embargo, los factores externos que influencian su cotidiano, muchas veces no permiten que ejerzan su voluntad de quedar con sus hijos.This purpose of this research was to verify the perception of the mother who has her child sick and hospitalized, about the importance of her permanence during the hospitalization time for the maintenance of the affective child-family link. Empiric data were collected with mother's of hospitalized children, who were accompanying the hospitalization of their children in a public hospital at the Cascavel city in the West of Paraná, Brazil, through semi-structured interviews

  16. O tornar-se cuidadora na senescência Conviertendo cuidador en la vejez Becoming caregiver in the old age

    Directory of Open Access Journals (Sweden)

    Elizabeth Braz

    2009-06-01

    Full Text Available Este estudo, desenvolvido no município de Cascavel PR, com senescentes cuidadoras domiciliares de idosos dependentes, teve como objetivo verificar os motivos que as levaram a executarem esse papel. A metodologia utilizada foi a qualitativa, e a Representação Social foi eleita como caminho teórico. Foram critérios de inclusão: sexo feminino; idade superior ou igual a 60 anos; cuidadores domiciliares principais de idosos dependentes por um período igual ou superior a três meses. A coleta de dados deu-se entre novembro de 2006 a janeiro de 2007. A população constituiu-se de 13 senescentes, sendo 46,1% esposas, o que atribuímos a própria representação social construída. A representação social também está relacionada à figura da mulher, geralmente a mais velha da família, viúva ou solteira. Sem recursos e tratados com desigualdade pelo sistema, o idoso cuidador e, nesse caso, a idosa cuidadora senescente tornam-se mais um ponto vulnerável em nossa sociedade.Estudio, desarrollado en la ciudad de Cascavel-PR, con los cuidadoras ancianas domiciliarios de dependientes envejecidos, tenía como el objetivo, verificar las razones para ejecutar este papel. La metodología usada era cualitativa y como camino teórico la representación social. Los criterios de inclusión fueran: sexo femenino, edad igual o superior a los sesenta años, cuidadores principales domiciliarios de dependientes envejecidos, por un período igual o superior los tres meses. La recogida de datos fue hecha desde noviembre de 2006 hasta enero de 2007. La población consistió en 13 envejecido, siendo 46.1% esposas, qué atribuimos la representación social construida. La representación social también está relacionada, a la figura de la mujer, generalmente más vieja de la familia, viuda o soltera. Tratados sin recursos, el cuidador envejecido y en este caso, la cuidadora envejecida, se convierte en más un punto vulnerable en nuestra sociedad.The present study

  17. Distribuição de frequência e temporal de chuvas intensas =

    Directory of Open Access Journals (Sweden)

    Luciana Espíndula de Quadros

    2011-07-01

    Full Text Available A distribuição generalizada de valores extremos (GEV tem tido grandeaceitação para discrição dos eventos máximos naturais. Estudos sobre o assunto mostram que a distribuição GEV ajusta valores mais próximos à realidade quando há necessidade de extrapolação dos dados amostrais para grandes períodos de retornos e com o intuito de contribuir para o planejamento de atividades que são influenciadas pela intensidade de água precipitada foi ajustado um modelo de distribuição de probabilidade de chuva intensa por meio da GEV, utilizando momentos LH para estimar seus parâmetros e o teste estatístico proposto por Wang (1998 para verificação da qualidade dos ajustes desenvolvidos noambiente Matlab. Analisaram-se séries históricas de precipitação máximas para diferentes durações obtidas de pluviográfos no município de Cascavel, Estado do Paraná. Além disso, as referidas séries foram ajustadas pela distribuição Gumbel para efeito de comparação coma GEV. Este trabalho mostra que a distribuição Gumbel subestima a distribuição GEV para grandes períodos de retorno.The generalized extreme value distribution (GEV has had great acceptance for discretion of the maximum natural events. Previous studies show that GEV distribution fits values closer to realitywhen there is need for extrapolation of sampling data for longer periods of returns. In order to contribute to the planning of activities that are influenced by the intensity of precipitated water, we adjusted a model of probability distribution of heavy rain through GEV, using LH moments for estimating its parameters and statistical test proposed by Wang (1998 for checking the quality of the adjustments developed in the Matlab. We analyzed historicaltime series of maximum rainfall for different durations obtained from rain gauges in the city of Cascavel, Paraná State. Moreover, these series were fitted by Gumbel distribution for purposes of comparison with the GEV

  18. Análise de um sistema de aquecimento de água para residências rurais, utilizando energia solar A water heating system analysis for rural residences, using solar energy

    Directory of Open Access Journals (Sweden)

    Luiz H. Basso

    2010-02-01

    Full Text Available A conscientização da importância do meio ambiente tem incentivado o estudo de novas fontes energéticas renováveis e menos poluentes. Dentre essas fontes, a energia solar destaca-se por ser perene e limpa. A utilização da energia solar em sistemas de aquecimento de água residencial rural pode colaborar com a economia de energia elétrica, base da matriz energética brasileira. Conhecer os fatores que influenciam na operação de um sistema de aquecimento de água por energia solar é importante na determinação de sua viabilidade técnica, visando a sua difusão em residências rurais. Para tanto, construiu-se um protótipo, no câmpus da Faculdade Assis Gurgacz, em Cascavel - PR, com características similares a um equipamento utilizado em residências para dois habitantes, para funcionar com circulação natural ou termossifão e sem auxílio de sistema de aquecimento complementar. O equipamento mostrou-se viável tecnicamente, alcançando a temperatura mínima para banho de 35 °C, sempre que a radiação solar foi superior a 3.500 Wh m-2, o que aconteceu para a maioria dos dias estudados.The awareness of the importance of the environment has stimulated the study of new renewed energy sources and less pollutant. Amongst these sources, solar energy stands alone for being perennial and clean. The use of solar energy in systems of agricultural residential water heating, can complement the economy of electric energy, base of the Brazilian energy matrix. Knowing the factors that influence the operation of a system of water heating by solar energy is important in determining their technical viabilities targeting their distribution in agricultural residences. To evaluate equipment of water heating for solar energy, a prototype was constructed in the campus of Assis Gurgacz College, in Cascavel,State of Paraná, Brazil, with similar characteristics to equipment used in residences for two inhabitants, to function with natural circulation or

  19. Alelopatia de cultivos de cobertura vegetal sobre plantas infestantes = Allelopathy of cover crop on weed plants

    Directory of Open Access Journals (Sweden)

    Luciene Kazue Tokura

    2006-07-01

    Full Text Available O presente trabalho avaliou o potencial alelopático de cultivos de cobertura vegetal de trigo, aveia preta, milheto, nabo forrageiro e colza sobre o desenvolvimento de plantas infestantes e verificou qual das coberturas vegetais exerce maior controle sobre as mesmas. Os cultivos de cobertura vegetal foram implantados sob preparo convencional (uma aração e uma gradagem no Núcleo Experimental de Engenharia Agrícola (NEEA, da Universidade Estadual do Oeste do Paraná (Unioeste, Cascavel, Estado do Paraná. Mensalmente, realizou-se o acompanhamento e identificação das plantas infestantes emersas nas áreas de cobertura vegetal no período de agosto de 2000 a agosto de 2001. Os resultados obtidos permitiram concluir que das espécies encontradas, o capim marmelada foi o que apresentou maior potencial alelopático e a erva-de-santa-maria o menor. As coberturasvegetais que apresentaram melhor controle do total de plantas infestantes presentes na área experimental, incluindo àquelas com reconhecido potencial alelopático, foram aveia preta, colza, nabo forrageiro e milheto.This work evaluated the cover crop allelopathic potential of wheat, black oat, pearl millet, turnip and rape on the development of weed plants. It also verified which cover crop has larger control on the weed plants. The cover crop was implanted under conventional tillage (one disk plowing plus one disk harrowing in the Experimental Nucleus of Agricultural Engineering (NEEA, of the State University of the West of Paraná (Unioeste, Cascavel, Paraná State. Monthly (from August 2000 to August 2001, weed plants identification in the cover crop area was made. Results showed that from the found species, the alexander grass was the one that presented larger allelopathic potential, and, the mexican-tea was the one that presented smaller control. The vegetable coverings that presented larger control of the total of weed plants in the experimental area, including those with

  20. Determinação da curva característica de um painel fotovoltaico em condições reais de trabalho = Determination of the characteristic curve of a photovoltaic panel in real conditions of work

    Directory of Open Access Journals (Sweden)

    Estor Gnoatto

    2005-07-01

    Full Text Available No presente trabalho foi desenvolvido um estudo sobre um sistemafotovoltaico autônomo, com armazenamento de energia, instalado na Estação Experimental Agrometeorológica da Universidade Estadual do Oeste do Paraná, Estado do Paraná, Campus Cascavel, a uma latitude 24º 59’ Sul, longitude de 53º 26’ Oeste, com altitude de 682 metros, sob condições reais de campo, durante o ano de 2002. Foi determinada a curva característica do painel, utilizando uma aquisição de dados um "micrologger" da Campbell Scientific-INC modelo CR10X, programado para realizar uma leitura por segundo de cada canal e armazenar a média aritmética de cinco minutos dos dados das componentes detemperatura de operação do painel; tensão e corrente do sistema fotovoltaico. Baseando-se nesses dados pode-se determinar que a curva característica do painel em condições reais de campo apresenta uma perda de 24% em seu rendimento em relação ao padrão STC (condições padrão de teste.The purpose of the present work was to develop the study of anautonomous photovoltaic system, with storage of energy, installed in the Agrometeorological Experimental Station of Universidade Estadual do Oeste do Paraná, State of Paraná, Campus Cascavel, at 24º 59’ latitude south, 53º26 longitude west, 682 meters of altitude, under real field conditions, during the year 2002. Efficacy of the photovoltaic panels has been evaluated under real field conditions, as well as to determine its characteristic linear fit. A 'micrologger' Campbell Scientific-INC model CR10X was used to acquire data, and it was programmed to accomplish a reading per second in each channel and to store the arithmetic average of data from five minutes of operational temperature of the panel, photovoltaic system tension and current. According to results, it could determined that the panel characteristic linear fit in real field conditions shows 24% less field than standard STC (Standard Test Conditions.

  1. Influência do espaçamento e população de plantas sobre doenças de final de ciclo e oídio e caracteres agronômicos em soja = Influence of row spacing and plant population on late season disease severity, powdery mildew and agronomic characters in soybean

    Directory of Open Access Journals (Sweden)

    Jorge Luiz Knebel

    2006-07-01

    Full Text Available Este trabalho foi conduzido em Cascavel, Estado do Paraná, na safra de2004/2005, utilizando a cultivar de soja CD 202, para avaliar a influência do espaçamento e da população de plantas sobre a severidade de (DFC doenças de final de ciclo (Septoria glycines e Cercospora kikuchii e oídio da soja (Microsphaera diffusa e caracteres agronômicos da cultura. Os tratamentos foram 3 espaçamentos (22,5; 45,0 e 67,5 cm e 3 populações (200; 400; e 600 mil plantas ha-1. As DFC tiveram menor severidade no espaçamento reduzido e na população mais baixa, enquanto a ocorrência de oídio não se alterou. Em todos os espa��amentos, quanto maior a população de plantas, maior a altura final e, conseqüentemente, maior o acamamento de plantas. O número de legumes/planta foi superior no maior espaçamento e reduziu com o aumento da população; o número de grãos/planta diminuiu com o aumento da população; e a produtividade foi superior no menor espaçamento.This study was carried out in Cascavel, Paraná State, during the growing season 2004/2005, using soybean cultivar CD 202. The aim was to evaluate the influence of row-spacing and plant population on lateseason disease severity (Septoria glycines and Cercospora kikuchii, soybean powdery mildew (Microsphaera diffusa and agronomic characters of the culture. The treatments were three row spacing (22.5; 45.0 and 67.5 cm and three plant populations (200; 400; and 600 thousand plants ha-1. Late season disease had smaller severity in the reduced spacing and in the lowest population, while the powdery mildew occurrence did not modify with these factors. In all spacing, as larger the plants density, larger the plants height and consequently larger the plants fallen. The number pods per plant was higher with the largest spacing and it decreased with the population increase; the number of grains per plant decreased with the population increase; and the productivity was higher in the smallest spacing.

  2. Molecular cloning and characterization of cDNAs encoding metalloproteinases from snake venom glands.

    Science.gov (United States)

    Jia, Ying; Pérez, John C

    2010-01-01

    Snake venom metalloproteinases (SVMPs) are a superfamily of zinc-dependent proteases and participate in a number of important biological, physiological and pathophysiological processes. In this work, we simultaneously amplified nine cDNAs encoding different classes of metalloproteinases from glands of four different snake species (Agkistrodon contortrix laticinctus, Crotalus atrox, Crotalus viridis viridis and Agkistrodon piscivorus leucostoma) by RT-PCR with a pair of primers. Among the encoded metalloproteinases, two enzymes (AclVMP-I and AplVMP-I), three enzymes (CaVMP-II, CvvVMP-II and AplVMP-II) and four enzymes (AclVMP-III, CaVMP-III, CvvVMP-III and AplVMP-III) with the characteristic motif (HEXXHXXGXXH) of metalloproteinase belong to type P-I, P-II and P-III enzymes, respectively. Disintegrin domains of CaVMP-II and CvvVMP-II from two Crotatus snakes contain RGD-motif whereas AplVMP-II from Agkistrodon snake has KGD-motif. Instead of R/KGD-motif within disintegrin domain of SVMP-II enzyme, CaVMP-III, CvvVMP-III and AplVMP-III enzymes contain SECD-motif, while AclVMP-III has DDCD-motif in their corresponding position of disintegrin-like domains. There are 12 Cys amino acids in cysterin-rich domains of each P-III enzyme. Moreover, a disintegrin precursor (AplDis) with RGD-motif also simultaneously amplified from the glands of A.p. leucostoma while amplifying AplVMP-II and AplVMP-III, which indicated that different types of SVMPs and related genes are present in a single species of snake and share a consensus sequence at the 3' and 5' untranslated regions. RT-PCR result also showed that P-III is highly expressed in Crotalus snakes than in Agkistrodon snakes. Aligning the deduced amino acid sequence of these enzymes with other SVMPs from GenBank database indicated that this is the first report on the isolation of cDNAs encoding P-II and P-III enzymes from C.v. viridis and A.p. leucostoma snakes. The availability of these SVMP sequences directly facilitated

  3. Determinação da curva característica de um painel fotovoltaico em condições reais de trabalho - DOI: 10.4025/actascitechnol.v27i2.1484

    Directory of Open Access Journals (Sweden)

    Estor Gnoatto

    2005-03-01

    Full Text Available No presente trabalho foi desenvolvido um estudo sobre um sistema fotovoltaico autônomo, com armazenamento de energia, instalado na Estação Experimental Agrometeorológica da Universidade Estadual do Oeste do Paraná, Estado do Paraná, Campus Cascavel, a uma latitude 24º 59’ Sul, longitude de 53º 26’ Oeste, com altitude de 682 metros, sob condições reais de campo, durante o ano de 2002. Foi determinada a curva característica do painel, utilizando uma aquisição de dados um "micrologger" da Campbell Scientific-INC modelo CR10X, programado para realizar uma leitura por segundo de cada canal e armazenar a média aritmética de cinco minutos dos dados das componentes de temperatura de operação do painel; tensão e corrente do sistema fotovoltaico. Baseando-se nesses dados pode-se determinar que a curva característica do painel em condições reais de campo apresenta uma perda de 24% em seu rendimento em relação ao padrão STC (condições padrão de teste

  4. Evaluation of the principle of coordination in primary health care of the child in Londrina-Pr

    Directory of Open Access Journals (Sweden)

    Gisleine Tíemi Souza

    2015-11-01

    Full Text Available Primary Care is a set of universally accessible services that promotes and protects health and prevents and treats diseases, and it is considered the initial access to the Health System. Four main essential attributes are present: accessibility, continuity, integrality and coordination. The coordination is the network of several health related services and actions that must be synchronized and continuous regardless of the location in which they occur. The goal of this study was to evaluate this coordination principle in Primary Care provided to children in 39 UBS (tr. From Basic Health Unit in the urban area of the city of Londrina. The research is multicentric (Londrina, Cascavel and João Pessoa, descriptive and quantitative – with use of PCATool-Brasil for children. In this instrument, coordination is subdivided in integration of care (which refers to the relation between Basic Health Care and specialties and the information system (which evaluates the health data and file availability. Amongst the 609 subjects submitted to interview, only 29.2% reported that a specialist examined the child. The coordination-integration of care score was 7.393 and the coordination-information system score was 7.620. From the eight questions concerning the coordination attribute that can be numeric-evaluated, three had scores below 6.6. The score should be higher than that to meet the concept of Primary Care. The conclusion is that the coordination attribute had a high score despite the three questions with a low score.

  5. O (DESINTERESSE DOS PROFESSORES FRENTE ÀS ATIVIDADES EM CONTRATURNO

    Directory of Open Access Journals (Sweden)

    Juliana Alves Da Silva Ubinski

    2015-01-01

    Full Text Available La educación a tiempo completo ha sido un tema tratado en la investigación educativa y en la elaboración de políticas educativas brasileñas. Algunos cambios en estas políticas han posibilitado innovaciones con respecto a una mayor permanencia del alumnado en el centro educativo, atendiendo algunas demandas sociales al respecto. La motivación de los profesores responsables de las actividades extra curriculares es esencial para el desarrollo efectivo de los estudiantes. Este trabajo tiene como objetivo presentar las percepciones de los profesores con respecto a las actividades curriculares complementadas con un horario extraclase. La investigación se llevó a cabo con profesores de una escuela pública de la ciudad de Cascavel, Paraná, Brasil, mediante la aplicación de un cuestionario. Los resultados obtenidos demuestran la necesidad de buscar un mayor compromiso de los profesores en relación con las actividades extracurriculares en las escuelas donde trabajan, para que así se sientan motivados a fomentar esta práctica.

  6. Effects of a labor gym program in mental workload of workers from rectory of UNIOESTE.

    Science.gov (United States)

    Galvão, Isabele Maia; Vilagra, José Mohamud; Moreira, Helenara Salvati Bertolossi; Moro, Antônio Renato Pereira; Cruz, Roberto Moraes; Reis, Pedro Ferreira; Moreira, Mauricio Bertolossi

    2012-01-01

    The present work is a quality-quantitative study of cause-effect type. The objective of this study was to investigate the effect of a Labor Gym (LG) program in mental workload of workers from rectory of the Universidade Estadual do Oeste do Paraná (Unioeste) Cascavel. The sample consisted of 35 employees. For data collection, it was used the NASA-TLX questionnaire to evaluate the mental workload of employees. Then, the participants performed sessions of LG, totaling 20 classes. After this period the employess were re-evaluated. To characterize the sample, data were tabulated and a descriptive analysis was performed using the SPSS 15.0. The interpretation of the NASA-TLX questionnaire was based on its own methodology. Before the LG sessions, the total average dimensions analyzed was 79 (sd: 11,05), representing a high rate of mental workload. After the period of LG, the total average of final mental workload decreased, but it remained with a high rate (x:75, sd: 13.42). In relation to the comparative analysis of the initial and final averages of mental workload, by t test (bipolar), there was no significant difference (p = 0.071668).

  7. Temporomandibular joint sounds and disc dislocations incidence after orotracheal intubation.

    Science.gov (United States)

    Rodrigues, Estela T; Suazo, Iván C; Guimarães, Antonio S

    2009-01-01

    The aim of this study was to analyze the temporomandibular joint (TMJ) disc displacement and articular sounds incidence after orotracheal intubation. A prospective cohort study was conducted in the Hospital Universitário do Oeste do Paraná (HUOP), in Cascavel, Brazil. 100 patients (aged 14-74 years, mean 44 years), 34 male and 66 female, in need of surgical procedure with orotracheal intubation were evaluated. The anterior disc displacement with reduction incidence and the nonclassifiable sounds incidence by the Research Diagnostic Criteria Axis I was evaluated in all patients after orotracheal intubation. The patients was evaluated one day before and until two days after the procedure. Eight percent present with anterior disc displacement with reduction and 10% presented nonclassifiable sounds after the orotracheal intubation. There was no correlation of any kind regarding gender related influence in the incidence of disc dislocations (P = 0.2591) and TMJ sounds (P = 0.487). Although anterior disc dislocations and TMJ sounds after anesthetic with orotracheal intubation presented a low incidence (8%-10%), it is recommended that the evaluation of TMJ signs and symptoms be done before the anesthetic procedure to take care with susceptible patients manipulation.

  8. Allelopathy of Camelina sativa Boiss. (Brassicaceae on germination and early development of Bidens pilosa (L. and Glycine max (L. Merr

    Directory of Open Access Journals (Sweden)

    Jéssica da Silva

    2011-12-01

    Full Text Available This study evaluated the possible presence of allelopathic potential in camelina, as well as its effect on germination and early growth of soybean seedlings and beggarticks, in order, to verify the possibility of cultivating it with soybeans in a culture rotation system and its use as a herbicide. The experiments were carried out at the Laboratory of Plant Physiology of Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil. The design was completely randomized and the evaluations were held daily. The parameters under analysis were: germination percentage, time and average speed of germination, and average root lenght. The data obtained underwent the F-Test and the mean values were compared through Tukey’s test, at a 5% probability level. The results confi rm the presence of allelopathic potential in camelina. It was found that this species can be considered an option for cultivating with soybean, due to the positive allelophatic interference caused in the culture and because it can be used in the control of weeds such as beggartick, having in mind that that it has delayed the development of the seedlings tested.

  9. Uso de álcool entre adolescentes escolares: um estudo-piloto

    Directory of Open Access Journals (Sweden)

    Luana Dallo

    2011-12-01

    Full Text Available O uso de álcool entre adolescentes é um tema que preocupa profissionais da saúde pelos prejuízos acarretados e pela displicência da sociedade quanto ao uso desta droga por essa faixa etária. Este artigo analisa o consumo de álcool entre alunos do ensino médio de três escolas de Cascavel-PR. O instrumento utilizado foi o Alcohol Use Disordens Identification Test (AUDIT, além de levantamento de informações sobre o nível socioeconômico, religião e o beber problemático de familiar. O resultado mostrou que 34,7% dos estudantes bebem de maneira arriscada, não havendo diferenças significativas por escola, gênero e nível socioeconômico. Ter familiar que bebe foi considerado fator de risco e ter religião, fator protetor. Esta pesquisa pode alertar a sociedade paranaense para a necessidade de estudos mais amplos, com o objetivo de desenvolver políticas públicas que incluam projetos de prevenção e intervenção dirigidos à população jovem.

  10. NURSING PROCESS, ACCORDING TO THE CONCEPTUAL MODEL OF WANDA HORTA, APPLIED TO A FAMILY MET BY THE REGIONAL CORE OF HOME CARE, DISTRITO FEDERAL.

    Directory of Open Access Journals (Sweden)

    Prince Vangeris Silva Fernandes de Lima

    2014-05-01

    Full Text Available The case report describes the nursing care, systematized according to the conceptual model of Wanda Horta, who was dismissed to a family accompanied by a core team of Regional home care, an administrative region of the Federal District through prioritization, based on the scale of Cascavel, a member of this for such a public service. The context part according to reports from the Discipline community health nursing in ' 02 and ' Stage, mandatory mode sold to students from the undergraduate program in nursing, University of Brasilia. At this juncture, described the history and nursing diagnoses, a welfare plan, as well as the prescription, the evolution and prognosis of nursing. In the residence there was the importance of specific health care provided not only by the nurse, but for the entire multidisciplinary team, customers with chronic clinical pictures and aggravated, but stable, that demand care that exceed those offered by primary health care. Seeking the completeness of the care, professionals and students involved started for the opportunity to visualize and better understand the patterns of relationships established between the client, family and healthcare professionals, with an emphasis on how the actors involved in this scenario mobilized in support of ailing client.

  11. Calorific value for compositions with biodiesel of fat chicken and diesel oil; Valor calorifico para composicoes com biodiesel da gordura de frango e oleo diesel

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Marcelo Jose da [Universidade de Campinas (FEAGRI/UNICAMP), SP (Brazil). Fac. de Engenharia Agricola], email: marcelo.jose@feagri.unicamp.br; Souza, Samuel N.M. de; Souza, Abel A. de; Martins, Gislaine I. [Universidade Estadual do Oeste do Parana (CCET/UNIOESTE), Cascavel, PR (Brazil). Centro de Ciencias Exatas e Tecnologicas], emails: ssouza@unioeste.br, abel.sza@hotmail.com, iastiaque@yahoo.com.br

    2011-07-01

    The biodiesel fuel is a renewable source of alternative fuel used in diesel cycle engines. The production of biodiesel involves the reaction of methanol with fatty acids of animal or vegetable. The production of biodiesel from chicken fat can be very attractive for some regions from Brazil with high poultry production, as in the Parana West and Santa Catarina West. In this study , the goal was the lower calorific value of the compositions between biodiesel and diesel oil: 100% Diesel oil (B0), 20% biodiesel (B20), 40% biodiesel (B40), 60% biodiesel (B60), 80% biodiesel (B80 ), 100% biodiesel (B100). The biodiesel used was acquired in the Centre for Development and Diffusion of technologies on the Assis Gurgacz College, in Cascavel city. The nominal production capacity of the unit is 900 liters on period of 8 hours. The model of the calorimeter used, was the E2K. The lower calorific value of B100 composition was 35.388 MJ kg-1 and the diesel oil was 41.299 MJ kg-1. With the measuring of the caloric value of six samples mix of diesel oil and biodiesel, was obtained a linear function decrease of the calorific value when increased it the proportion of biodiesel from chicken fat into fuel. (author)

  12. Acesso ao cuidado à saúde da criança em serviços de atenção primária

    Directory of Open Access Journals (Sweden)

    Rosane Meire Munhak da Silva

    2014-10-01

    Full Text Available Realizou-se uma pesquisa de abordagem qualitativa com base no referencial metodológico da hermenêutica dialética, com o objetivo de identificar o atributo acesso da atenção primária para a resolução dos problemas de saúde de crianças menores de um ano a partir dos relatos de pais e cuidadores. Envolveram-se 16 cuidadores de crianças atendidas em unidades de pronto atendimento de Cascavel-PR, em 2010. Foram reconhecidas quatro categorias temáticas: Aconselhamento familiar ao buscar atenção à saúde da criança; Ausência de acolhimento ao primeiro contato; Presença de classificação de risco para atenção à saúde da criança; Barreiras que impedem o acesso à atenção à saúde. Concluiu-se que as famílias exibiram dificuldades para alcançar resolutividade aos problemas de saúde dos filhos, mediante a falta de acesso aos serviços de atenção primária.

  13. Comportamento sexual de risco entre estudantes universitárias dos cursos de ciências da saúde Risky sexual behavior among university students in health science courses

    Directory of Open Access Journals (Sweden)

    Angela Maria Moser

    2007-04-01

    Full Text Available OBJETIVO: Avaliar o comportamento sexual de estudantes do sexo feminino do Setor de Ciências da Saúde da Universidade Federal do Paraná e do Setor de Ciências Biológicas e da Saúde da Universidade Estadual do Oeste do Paraná, Campus Cascavel. MÉTODOS: Participaram todas as estudantes matriculadas nos referidos setores, no mês de junho de 2001, com idade entre 18 e 24 anos completos, sendo que a amostra final foi constituída por 572 estudantes em Curitiba e 395 em Cascavel. Foram avaliados a idade, relacionamento familiar, religiosidade, participação em aulas de educação sexual, idade de início de atividade sexual, uso de métodos anticoncepcionais e preservativos, e número de parceiros para avaliar a conduta sexual. Sexo seguro foi definido pelo uso de preservativo em todas ou na maioria das relações sexuais pelas estudantes monogâmicas, incluindo as estudantes poligâmicas com uso de preservativo em todas as relações sexuais. Sexo inseguro foi definido pelo uso ocasional de preservativo pelas estudantes monogâmicas, e pelo não uso sistemático entre as estudantes poligâmicas. O instrumento de coleta de dados foi um questionário auto-respondido. A análise dos dados foi feita no SPSS, utilizando o teste do Qui-quadrado de Pearson e Yates, teste do p de Wilcoxon-Gehan, análise bivariada e regressão logística. RESULTADOS: Não houve diferença significativa na conduta sexual de risco entre as estudantes e a cidade de estudo. Cerca de 50% das estudantes de 18 a 20 anos e 70% das de 21 a 24 anos tinham vida sexual ativa. A abstinência esteve associada a menor idade, maior freqüência a culto religioso e ao bom relacionamento das estudantes com os pais. A prática de sexo seguro esteve diretamente associada a menor idade e a morar fora da residência da família. Ter participado de aulas de educação sexual esteve associada a maior abstinência, mas não a sexo seguro, e a relação não se manteve na an

  14. EXPERIÊNCIAS, SEMELHANÇAS E ALGUMAS PARTICULARIDADES NA ORGANIZAÇÃO DO TRABALHO NA CATAÇÃO DE MATERIAIS RECICLÁVEIS NAS COOPERATIVAS DE RECICLAGEM: COAAFI E COOTACAR

    Directory of Open Access Journals (Sweden)

    Solange Queiroz Ribeiro

    2013-12-01

    Full Text Available O artigo tem como foco principal entender o trabalho coletivo de dois grupos de trabalhadores catadores organizados em cooperativas de reciclagem, mais propriamente, visando apreender a organização do trabalho como enfrentamento as questões conflitantes do capital, assim como, a luta para além da organização interna e a trajetória dos trabalhadores na catação dos resíduos sólidos, compostos por materiais recicláveis, que sobrevivem a partir do que é considerado lixo para alguns e meio de sobrevivência para outros, nos municípios de Cascavel e Foz do Iguaçu, região Oeste do Paraná. O estudo revela sua importância no sentido de entender a dinâmica geográfica do trabalho na catação dos resíduos, pois a Geografia enquanto ciência humana permite entender suas relações sociais, assim como é o caso destes trabalhadores. Palavras chave: Organização do trabalho; trabalho; catação de recicláveis; Geografia. 

  15. A water heating system analysis for rural residences, using solar energy; Analise de um sistema de aquecimento de agua para residencias rurais, utilizando energia solar

    Energy Technology Data Exchange (ETDEWEB)

    Basso, Luiz H.; Souza, Samuel N.M. de; Siqueira, Jair A.C.; Nogueira, Carlos E.C.; Santos, Reginaldo F. [Universidade Estadual do Oeste do Parana (UNIOESTE), Cascavel, PR (Brazil). Programa de Pos-Graduacao em Engenharia Agricola], emails: melegsouza@yahoo.com, ssouza@unioeste.br, jairsiqueira@unioeste.br, cecn1@yahoo.com.br, rfsantos@unioeste.br

    2010-01-15

    The awareness of the importance of the environment has stimulated the study of new renewed energy sources and less pollutant. Amongst these sources, solar energy stands alone for being perennial and clean. The use of solar energy in systems of agricultural residential water heating, can complement the economy of electric energy, base of the Brazilian energy matrix. Knowing the factors that influence the operation of a system of water heating by solar energy is important in determining their technical viabilities targeting their distribution in agricultural residences. To evaluate equipment of water heating for solar energy, a prototype was constructed in the campus of Assis Gurgacz College, in Cascavel,State of Parana, Brazil, with similar characteristics to equipment used in residences for two inhabitants, to function with natural circulation or thermo siphon and without help of a complementary heating system. The equipment revealed technical viability, reaching the minimum temperature for shower, of 35 deg C, whenever the solar radiation was above the 3,500 Wh m{sup -2}, for the majority of the studied days. (author)

  16. Mudanças no trabalho e na vida de bancários portadores de Lesões por Esforços Repetitivos: LER

    Directory of Open Access Journals (Sweden)

    Neide Tiemi Murofuse

    Full Text Available A LER constitui-se num dos graves problemas de saúde dos trabalhadores na atualidade. Com o objetivo de analisar as principais mudanças no trabalho e na vida dos portadores de LER de membros de uma Associação de Portadores de LER de Cascavel-PR, foi desenvolvido o estudo, de natureza qualitativa. Foram realizadas entrevistas semi-estruturadas, com nove bancários. Os primeiros sintomas surgiram no período coincidente com a automação e a introdução de novas tecnologias no sistema financeiro brasileiro e as mudanças na organização do trabalho. Aumento do volume de trabalho e da digitação, horas-extra, ausências de pausas, monotonia, repetitividade, ritmo intenso e controle da produtividade e tensão constituíram os principais elementos. O agravamento das lesões afetou a capacidade produtiva e implicou na dependência para execução de atividades no trabalho e fora dele. O desafio está em garantir os direitos desses trabalhadores.

  17. Influenza em animais heterotérmicos Influenza in heterothermics

    OpenAIRE

    Dalva Assunção Portari Mancini; Rita Maria Zucatelli Mendonça; Aurora Marques Cianciarullo; Leonardo Setsuo Kobashi; Hermínio Gomes Trindade; Wilson Fernandes; José Ricardo Pinto

    2004-01-01

    O objetivo foi pesquisar Ortomyxovirus em animais heterotérmicos. Coletou-se sangue de serpentes dos gêneros Bothrops e Crotalus e de sapo e rãs dos gêneros Bufo e Rana, para a detecção dos receptores de hemácias e anticorpos específicos, ao vírus influenza, pelos testes de hemaglutinação e inibição da hemaglutinação, respectivamente. Pelo teste de hemaglutinação, verificou-se que serpentes e sapos em cativeiro apresentaram receptores em suas hemácias para o vírus influenza, humano e eqüino d...

  18. Influenza em animais heterotérmicos

    OpenAIRE

    Mancini, Dalva Assunção Portari; Mendonça, Rita Maria Zucatelli; Cianciarullo, Aurora Marques; Kobashi,Leonardo Setsuo; Trindade,Hermínio Gomes; Fernandes, Wilson; Pinto,José Ricardo

    2004-01-01

    O objetivo foi pesquisar Ortomyxovirus em animais heterotérmicos. Coletou-se sangue de serpentes dos gêneros Bothrops e Crotalus e de sapo e rãs dos gêneros Bufo e Rana, para a detecção dos receptores de hemácias e anticorpos específicos, ao vírus influenza, pelos testes de hemaglutinação e inibição da hemaglutinação, respectivamente. Pelo teste de hemaglutinação, verificou-se que serpentes e sapos em cativeiro apresentaram receptores em suas hemácias para o vírus influenza, humano e eqüino d...

  19. Trimeresurus venom inhibition of anti-HPA-1a and anti-HPA-1b antibody binding to human platelets.

    Science.gov (United States)

    Wlodar, S J; Stone, D L; Sinor, L T

    1995-01-01

    A solid-phase red cell adherence assay was used to demonstrate the specific inhibitory effect of seven species of Trimeresurus snake venom on the binding of HPA-1a- and HPA-1b-specific platelet antibodies. Trimeresurus venom did not inhibit the binding of HLA-, HPA-3a-, HPA-3b-, HPA-4a-, HPA-5a-, and HPA-5b-specific platelet antibodies. Venom from other genera of snakes, including representatives from Agkistrodon, Ancistrodon, Bitis, Bothrops, Bungarus, Causus, Crotalus, Dendroaspis, Ecis, Micrurus, Naja, Notechis, Ophiophagus, Pseudechis, Sepedon (Hemachatus), and Vipera, all failed to specifically inhibit anti-HPA-1a and HPA-1b binding. These results may indicate that the component in Trimeresurus snake venom previously reported to bind to the platelet GPIIb-IIIa complex, inhibiting fibrinogen binding, binds close to the HPA-1a and HPA-1b epitopes.

  20. Physiological importance of the coronary arterial blood supply to the rattlesnake heart

    DEFF Research Database (Denmark)

    Hagensen, Mette; Abe, Augusto S.; Falk, Erling;

    2008-01-01

    supply to the outer compact layer in untreated snakes. Electrocardiogram (ECG), blood pressure (Psys) and heart rate (fH) were measured at rest and during enforced activity at day 1 and 4. Four days after occlusion of the coronary circulation, the snakes could still maintain a Psys and fH of 5.2±0.2 k......Pa and 58.2±2.2 beats min-1, respectively, during activity and the ECG was not affected. This was not different from sham-operated snakes. Thus, while the outer compact layer of the rattlesnake heart clearly has an extensive coronary supply, rattlesnakes sustain a high blood pressure and heart rate during...... remains unknown. In the present study we investigate the effects of permanent coronary artery occlusion in the South American rattlesnake (Crotalus durissus) on the ability to maintain heart rate and blood pressure at rest and during short term activity. We used colored silicone rubber (Microfil...

  1. Oxygenation properties and isoform diversity of snake hemoglobins

    DEFF Research Database (Denmark)

    Storz, Jay F.; Natarajan, Chandrasekhar; Moriyama, Hideaki

    2015-01-01

    Available data suggest that snake hemoglobins (Hbs) are characterized by a combination of unusual structural and functional properties relative to the Hbs of other amniote vertebrates, including oxygenation-linked tetramer- dimer dissociation. However, standardized comparative data are lacking...... for snake Hbs, and the Hb isoform composition of snake red blood cells has not been systematically characterized. Here we present the results of an integrated analysis of snake Hbs and the underlying - and -type globin genes to characterize 1) Hb isoform composition of definitive erythrocytes, and 2......) the oxygenation properties of isolated isoforms as well as composite hemolysates. We used species from three families as subjects for experimental studies of Hb function: South American rattlesnake, Crotalus durissus (Viperidae); Indian python, Python molurus (Pythonidae); and yellow-bellied sea snake, Pelamis...

  2. Sidewinding with minimal slip: Snake and robot ascent of sandy slopes

    CERN Document Server

    Marvi, Hamidreza; Gravish, Nick; Astley, Henry; Travers, Matthew; Hatton, Ross L; Mendelson, Joseph R; Choset, Howie; Hu, David L; Goldman, Daniel I

    2014-01-01

    Limbless organisms such as snakes can navigate nearly all terrain. In particular, desert-dwelling sidewinder rattlesnakes (Crotalus cerastes) operate effectively on inclined granular media (such as sand dunes) that induce failure in field-tested limbless robots through slipping and pitching. Our laboratory experiments reveal that as granular incline angle increases, sidewinder rattlesnakes increase the length of their body in contact with the sand. Implementing this strategy in a physical robot model of the snake enables the device to ascend sandy slopes close to the angle of maximum slope stability. Plate drag experiments demonstrate that granular yield stresses decrease with increasing incline angle. Together, these three approaches demonstrate how sidewinding with contact-length control mitigates failure on granular media.

  3. Minimal volume regulation after shrinkage of red blood cells from five species of reptiles

    DEFF Research Database (Denmark)

    Kristensen, Karina; Berenbrink, Michael; Koldkjær, Pia

    2008-01-01

    Red blood cells (RBCs) from most vertebrates restore volume upon hypertonic shrinkage and the mechanisms underlying this regulatory volume increase (RVI) have been studied extensively in these cells. Despite the phylogenetically interesting position of reptiles, very little is known about their red...... cell function. The present study demonstrates that oxygenated RBCs in all major groups of reptiles exhibit no or a very reduced RVI upon ~ 25% calculated hyperosmotic shrinkage. Thus, RBCs from the snakes Crotalus durissus and Python regius, the turtle Trachemys scripta and the alligator Alligator...... was not characterized. It seems, therefore, that the RVI response based on NHE activation was lost among the early sauropsids that gave rise to modern reptiles and birds, while it was retained in mammals. An RVI response has then reappeared in birds, but based on activation of the NKCC. Alternatively, the absence...

  4. Evaluation of snake venom phospholipase A{sub 2}: hydrolysis of non-natural esters

    Energy Technology Data Exchange (ETDEWEB)

    Pirolla, Renan A.S.; Baldasso, Paulo A.; Marangoni, Sergio; Moran, Paulo J.S.; Rodrigues, Jose Augusto R., E-mail: jaugusto@iqm.unicamp.b [University of Campinas (UNICAMP), SP (Brazil). Inst. of Chemistry. Dept. of Organic Chemistry

    2011-07-01

    Phospholipase A2 from the rattlesnake Crotalus durissus terrificus was employed for the first time to test its enantioselectivity on the hydrolysis of different non-natural esters. It was observed that the structure of this small enzyme is restrictive in the choice of its lipase action with non-natural substrates. Two forms of the enzyme were used; free and as its cross-linked enzyme aggregate (CLEA). With all substrates, the free enzyme showed activity similar to the CLEA preparation. The advantage of the CLEA phospholipase is the possibility to reuse it in several consecutive reactions without a decrease of activity and selectivity with good but higher yields and ee than with the free enzyme. (author)

  5. {sup 131}I-CRTX internal dosimetry: animal model and human extrapolation

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Henrique Martins de; Ferreira, Andrea Vidal; Soares, Marcella Araugio; Silveira, Marina Bicalho; Santos, Raquel Gouvea dos [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN-CNEN-MG), Belo Horizonte, MG (Brazil)], e-mail: hma@cdtn.br

    2009-07-01

    Snake venoms molecules have been shown to play a role not only in the survival and proliferation of tumor cells but also in the processes of tumor cell adhesion, migration and angiogenesis. {sup 125}I-Crtx, a radiolabeled version of a peptide derived from Crotalus durissus terrificus snake venom, specifically binds to tumor and triggers apoptotic signalling. At the present work, {sup 125}I-Crtx biokinetic data (evaluated in mice bearing Erlich tumor) were treated by MIRD formalism to perform Internal Dosimetry studies. Doses in several organs of mice were determinate, as well as in implanted tumor, for {sup 131}I-Crtx. Doses results obtained for animal model were extrapolated to humans assuming a similar concentration ratio among various tissues between mouse and human. In the extrapolation, it was used human organ masses from Cristy/Eckerman phantom. Both penetrating and non-penetrating radiation from {sup 131}I in the tissue were considered in dose calculations. (author)

  6. Effects of gamma radiation on snake venoms

    Energy Technology Data Exchange (ETDEWEB)

    Nascimento, N.; Spencer, P.J.; Andrade, H.F.; Guarnieri, M.C.; Rogero, J.R

    1998-06-01

    Ionizing radiation is able to detoxify several venoms, including snake venoms, without affecting significantly their immunogenic properties. In order to elucidate this phenomena, we conceived a comparative pharmacological study between native and irradiated (2,000 Gy) crotoxin, the main toxin of the South American rattlesnake Crotalus durissus terrificus. Crotoxin was isolated and purified by molecular exclusion chromatography, pI precipitation and, subsequently submitted to irradiation. Gel filtration of the irradiated toxin resulted in some high molecular weight aggregates formation. Crotoxin toxicity decreased two folds after irradiation, as determined by LD{sub 50} in mice. Native and irradiated crotoxin biodistribution ocurred in the same general manner, with renal elimination. However, in contrast to irradiated crotoxin, the native form was initially retained in kidneys. A later concentration (2-3 hr) appeared in phagocytic mononuclear cells rich organs (liver and spleen) and neural junction rich organs (muscle and brain)

  7. Comparative morphocytologic study of native and irradiated crotoxin on CBA/J mice tissues and cells; Estudo morfocitologico comparativo de crotoxina nativa e irradiada em tecidos e celulas de camundongos CBA/J

    Energy Technology Data Exchange (ETDEWEB)

    Cardi, Bruno A.

    1995-12-31

    Snake venoms submitted to gamma radiation are better immunogens, have less toxic activity and maintain immunogenic and antigenic properties, favoring its use in anti-sera production which is the main therapy in human accidents. Severe human accidents attributed to neurotoxicity, are caused by Crotalus durissus terrificus, which main toxin - crotoxin - acts on neuro muscular junctions, as detected by electrophysiological studies and could be detoxified by gamma radiation. In order to evaluate the biological effects of this irradiated protein, we analyzed, at morphological and immunohistochemical levels, organs and cells from CBA/J mice challenged with crude venom or purified crotoxin, in an acute intoxication model. Both toxins were submitted to 2 kGy irradiation with a Co-60 source, in water solution. (author). 140 refs., 10 figs., 1 tab.

  8. Influence of the main reactive species formed during the detoxication process of toxins by ionizing radiation; Influencia das principais especies reativas formadas durante o processo de destoxicacao de toxinas por radiacao ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Murilo Casare da

    2003-07-01

    Ionizing radiation has been satisfactorily employed for venoms detoxification. In this report, the radiation was employed to verify the effects caused by the radiolysis products of water on two toxins (Crotoxin and Crotamine) purified from Crotalus durissus terrificus venom. These effects were analyzed using some substances called 'scavengers', those substances competes for specific reactive species hindering them to act on the toxins molecules. In order to study the possible structural damages caused on the toxins, UV spectra, fluorescence, mass spectrometry, enzymatic activity were employed. In addition, biochemical techniques were employed to evaluate the decrease of toxicity and the immunogenicity of toxins before and after the irradiation. Our results indicate that the irradiation promotes structural damages, even at low doses. These modifications lead to a gradual decrease in toxicity, however, the immunogenic properties of the toxins are preserved. (author)

  9. A new heterologous fibrin sealant as a scaffold to cartilage repair-Experimental study and preliminary results.

    Science.gov (United States)

    de Barros, Caio Nunes; Miluzzi Yamada, Ana Lúcia; Junior, Rui Seabra F; Barraviera, Benedito; Hussni, Carlos Alberto; de Souza, Jaqueline Brandão; Watanabe, Marcos Jun; Rodrigues, Celso Antônio; Garcia Alves, Ana Liz

    2016-07-01

    Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study.

  10. Neutralization of haemorrhagic activity of viper venoms by 1-(3-dimethylaminopropyl)-1-(4-fluorophenyl)-3-oxo-1,3-dihydroisobenzofuran-5-carbonitrile.

    Science.gov (United States)

    Sunitha, Kabburalli; Hemshekhar, Mahadevappa; Gaonkar, Santosh Laxman; Sebastin Santhosh, Martin; Suresh Kumar, Muthuvel; Basappa; Priya, Babu Shubha; Kemparaju, Kempaiah; Rangappa, Kanchugarakoppal Subbegowda; Nanjunda Swamy, Shivananju; Girish, Kesturu Subbaiah

    2011-10-01

    Viper envenomation undeniably induces brutal local manifestations such as haemorrhage, oedema and necrosis involving massive degradation of extracellular matrix at the bitten region and many a times results in dangerous systemic haemorrhage including pulmonary shock. Snake venom metalloproteases (SVMPs) are being considered to be the primary culprits for the venom-induced haemorrhage. As a consequence, the venom researchers and medical practitioners are in deliberate quest of SVMP inhibitors. In this study, we evaluated the inhibitory effect of 1-(3-dimethylaminopropyl)-1-(4-fluorophenyl)-3-oxo-1,3-dihydroisobenzofuran-5-carbonitrile (DFD) on viper venom-induced haemorrhagic and PLA(2) activities. DFD effectively neutralized the haemorrhagic activity of the medically important viper venoms such as Echis carinatus, Echis ocelatus, Echis carinatus sochureki, Echis carinatus leakeyi and Crotalus atrox in a dose-dependent manner. The histological examinations revealed that the compound DFD effectively neutralizes the basement membrane degradation, and accumulation of inflammatory leucocytes at the site of Echis carinatus venom injection further confirms the inhibition of haemorrhagic activity. In addition, DFD dose dependently inhibited the PLA(2) activities of Crotalus atrox and E. c. leakeyi venoms. According to the docking studies, DFD binds to hydrophobic pocket of SVMP with the ki of 19.26 × 10(-9) (kcal/mol) without chelating Zn(2+) in the active site. It is concluded that the clinically approved inhibitors of haemorrhagins could be used as a potent first-aid agent in snakebite management. Furthermore, a high degree of structural and functional homology between SVMPs and their relatives, the MMPs, suggests that DFD analogues may find immense value in the regulation of multifactorial pathological conditions like inflammation, cancer and wound healing.

  11. Snake co-occurrence patterns are best explained by habitat and hypothesized effects of interspecific interactions.

    Science.gov (United States)

    Steen, David A; McClure, Christopher J W; Brock, Jean C; Craig Rudolph, D; Pierce, Josh B; Lee, James R; Jeffrey Humphries, W; Gregory, Beau B; Sutton, William B; Smith, Lora L; Baxley, Danna L; Stevenson, Dirk J; Guyer, Craig

    2014-01-01

    Snakes often occur in species-rich assemblages, and sympatry is thought to be facilitated primarily by low diet overlap, not interspecific interactions. We selected, a priori, three species pairs consisting of species that are morphologically and taxonomically similar and may therefore be likely to engage in interspecific, consumptive competition. We then examined a large-scale database of snake detection/nondetection data and used occupancy modelling to determine whether these species occur together more or less frequently than expected by chance while accounting for variation in detection probability among species and incorporating important habitat categories in the models. For some snakes, we obtained evidence that the probabilities that habitat patches are used are influenced by the presence of potentially competing congeneric species. Specifically, timber rattlesnakes (Crotalus horridus) were less likely than expected by chance to use areas that also contained eastern diamond-backed rattlesnakes (Crotalus adamanteus) when the proportion of evergreen forest was relatively high. Otherwise, they occurred together more often than expected by chance. Complex relationships were revealed between habitat use, detection probabilities and occupancy probabilities of North American racers (Coluber constrictor) and coachwhips (Coluber flagellum) that indicated the probability of competitive exclusion increased with increasing area of grassland habitat, although there was some model uncertainty. Cornsnakes (Pantherophis guttatus or Pantherophis slowinskii) and ratsnakes (Pantherophis alleghaniensis, Pantherophis spiloides, or Pantherophis obsoletus) exhibited differences in habitat selection, but we obtained no evidence that patterns of use for this species pair were influenced by current interspecific interactions. Overall, our results are consistent with the hypothesis that competitive interactions influence snake assemblage composition; the strength of these effects was

  12. Acidente crotálico na infância: aspectos clínicos, laboratoriais, epidemiológicos e abordagem terapêutica

    Directory of Open Access Journals (Sweden)

    Palmira Cupo

    1991-06-01

    Full Text Available Foram atendidas no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto/USP, durante os anos de 1980 a 1989, 21 crianças vítimas de acidente crotálico, 16 das quais apresentaram sinais clínicos de envenenamento grave à admissão e 5, de envenenamento moderado. Quatro pacientes (20% desenvolveram necrose tubular aguda, dois dos quais necessitaram métodos dialíticos. Um paciente (5% foi a óbito no 13.° dia do acidente, após intercorrências graves, como hemorragia digestiva e insuficiência respiratória aguda. São apresentadas as características clínicas, laboratoriais e epidemiológicas do acidente crotálico nesses pacientes, enfatizando os conhecimentos atuais sobre a fisiopatologia do envenenamento por Crotalus durissus terrificus. Ao mesmo tempo, pretendeu-se contribuir para a correta abordagem terapêutica, no que diz respeito ao emprego adequado do soro antiveneno e à prevenção da necrose tubular aguda, a mais séria e grave complicação do acidente crotálico.At the Hospital of Clinics of the Faculty of Medicine of Ribeirão Preto/USP during the years 1980-1989 21 children have been attended after rattlesnake bite: 16 severe and 5 with moderate envenomation. Four (20% developed acute tubular necrosis 2 necessitating dialysis. One patient died 13 days after the bite and grave complications including digestive hemorrhage and acute respiratory insuficiency. All patients preserved clinical laboratory and epidemiological characteristics of Crotalus durissus terrificus envenomation. We also comment on the correct management of such patients specially related to antivenin dosage and the prevention ofacute tubular the most serious complication of such an accident.

  13. A importância dos acidentes ofídicos como causa de mortes em bovinos no Brasil The importance of snake bites as cause of cattle death in Brazil

    Directory of Open Access Journals (Sweden)

    Carlos Hubinger Tokarnia

    2006-06-01

    Full Text Available A revisão da literatura pertinente indica que as opiniões sobre a importância dos acidentes ofídicos, como causa de mortes em bovinos no Brasil, são divergentes no meio veterinário. Enquanto alguns acreditam que são pouco importantes, ou que têm menor significado do que lhes é atribuído, outros são da opinião que esses acidentes são freqüentes. Verificou-se que só foi relatado diagnóstico fundamentado de dois casos fatais de envenenamento por Bothrops spp em bovinos, e de nenhum por Crotalus spp. Um questionário por nós submetido a patologistas e clínicos veterinários que atuam em diversos Estados do país, revelou apenas raros casos suspeitos de envenenamento ofídico fatal em bovinos no Brasil. Em nossas viagens de estudo e nos trabalhos de diagnóstico nunca estabelecemos o diagnóstico de morte por acidente ofídico em bovinos. Os casos tidos como envenenamento ofídico, na sua grande maioria, são apenas suposições, sem embasamento. Esses "diagnósticos", em geral, são feitos à distância dos animais que morreram, à revelia de exame clínico, necropsia e estudo histopatológico. Importante foi a constatação de que, no Brasil, embora algumas serpentes do gênero Bothrops possam, teoricamente, produzir quantidades suficientes de veneno para matar um bovino adulto, em experimentos realizados, apenas Bothrops alternatus foi capaz de levar a morte um dos três bovinos experimentalmente por ela picados; esse animal tinha apenas 279 kg. Já as serpentes do gênero Crotalus poderiam inocular quantidades letais de veneno para bovinos adultos. Mesmo assim, tanto para Bothrops spp, como para Crotalus spp, há que se considerar que as serpentes, em geral, só inoculam parte do veneno disponível. Esse estudo indica que é necessário melhor investigar as mortes suspeitas de terem sido causadas por acidente ofídico em bovinos no Brasil. O estabelecimento do diagnóstico de morte por envenenamento ofídico, porém, só é poss

  14. A tilapicultura e seus insumos, relações econômicas Tilapia culture and its inputs, economic relations

    Directory of Open Access Journals (Sweden)

    João Donato Scorvo Filho

    2010-07-01

    Full Text Available A aquicultura brasileira é a segunda (destaque para o Chile em produção sul-americana, apoiada basicamente nas cadeias de produção da tilapicultura e da carcinicultura. O Brasil produziu, em 2007, 95.691,0 toneladas de tilápia, o que representa 45% da produção da aquicultura continental. A aquicultura formada por pequenos e médios produtores vem mostrando, nos últimos anos, mudança nos sistemas de criação. Até a o final da década de 90, ela se baseava no sistema semi-intensivo em viveiros escavados e de barragens. A partir do ano de 2000, surge, com força, a tilapicultura em tanques-rede, principalmente em águas da União (grandes reservatórios de hidroelétricas e açudes da Região Nordeste. Esta mudança acarretou alterações na cadeia de produção, uma vez que são necessários insumos adequados ao sistema: rações específicas, material genético compatível com a criação e formas de escoamento da produção, uma vez que no novo sistema apresenta maior escala de produção. O sistema agroindustrial do pescado abrange dois sistemas de produção distintos: a pesca (sistema extrativo e a aquicultura (sistema produtivo. Alguns elos deste sistema são compartilhados pelos dois sistemas, porém ração e material genético são exclusivos da aquicultura. Nos elos de transformação, distribuição e comercialização, ambos os sistemas se relacionam e, muitas vezes, concorrem entre si. Atualmente, o Brasil possui linhagens melhoradas geneticamente, em contínuo processo de melhoramento e adaptaçao às nossas condições. A atividade de tilapicultura vem passando, desde as décadas passadas, por um processo de profissionalização, com o produtor mais atento ao manejo e aos insumos utilizados. Hoje, é possível escolher o insumo que mais se adapta ao manejo empregado pelo produtor, porém isso não é é uma prática generalizada quando se considera seu preço de venda.The Brazilian aquaculture is the second (Chile is the

  15. Efeito da infecção precoce por Begomovirus com genoma bipartido em características de frutos de tomate industrial Effect of early infection by a bipartite Begomovirus species on processing tomato fruit characteristics

    Directory of Open Access Journals (Sweden)

    Leonardo de B. Giordano

    2005-07-01

    Full Text Available Dados a respeito dos efeitos da infecção por espécies sul-americanas de Begomovirus com genoma bipartido na fisiologia da produção do tomateiro são ainda escassos na literatura. O presente trabalho foi realizado visando estimar o impacto da infecção precoce em parâmetros de produtividade e teor de sólidos solúveis (ºBrix utilizando uma cultivar de tomateiro para processamento industrial suscetível a Begomovirus. A inoculação controlada das mudas (18 dias após semeadura foi realizada inicialmente em casa de vegetação usando moscas-brancas (Bemisia tabaci biótipo B virulíferas, sendo as mudas expostas aos insetos por quatro dias. Um grupo de mudas não inoculadas (controles foi mantido isolado em uma casa de vegetação livre do inseto vetor. Plantas inoculadas e não inoculadas foram simultaneamente transplantadas em campo e dispostas em um delineamento de blocos ao acaso com quatro repetições com dez plantas por parcela. A análise de seqüência de nucleotídeos de parte do genoma do DNA-A do vírus utilizado na inoculação indicou ser um isolado distinto, mas geneticamente relacionado com Tomato chlorotic mottle virus (uma das espécies do complexo de Begomovirus infectando tomateiro no Brasil. Foi observada diferença significativa para produção total, sendo 109,0 t/ha nas plantas controle e 48,2 t/ha nas mudas submetidas à inoculação precoce; tendo-se redução de aproximadamente 60% na produtividade. A diferença mais acentuada foi observada para o parâmetro número de frutos por planta. As plantas não infectadas apresentaram uma média de 66 frutos, enquanto que nas plantas infectadas esta média foi de 38 frutos. O peso médio e o teor de sólidos solúveis dos frutos não foram significativamente influenciados pela infeção precoce. Desta forma, existe a possibilidade de reduzir perdas de produtividade devido à infeção de Begomovirus por meio do manejo adequado do sistema de produção, incluindo

  16. Teor de nitrato em alface hidropônica em função de vazões e períodos de pós-colheita Nitrate content in hydroponic lettuce in function of flow rate and post-harvest periods

    Directory of Open Access Journals (Sweden)

    Antonio Aprígio

    2012-09-01

    Full Text Available O objetivo do trabalho foi determinar o teor de nitrato (NO3- presente em plantas de alface (Lactuca sativa L. da cultivar Vera, conduzida por hidroponia, em função de vazões e períodos de pós-colheita. O experimento foi realizado no município de Cascavel, PR, em casa de vegetação da Universidade Estadual do Oeste do Paraná (UNIOESTE. As plantas foram nutridas mediante utilização de uma solução nutritiva caracterizada por possuir condutividade elétrica média de 1,2 dS m-1 e que, ao percorrer toda a bancada de cultivo, retornava ao tanque de irrigação e reiniciava o processo, constituindo, desta forma, um sistema fechado. No experimento, utilizou-se o delineamento inteiramente casualizado com oito repetições que foram representadas, individualmente, por duas plantas de alface, no esquema fatorial (3 x 4. Os tratamentos resultaram da combinação entre três vazões (0,5; 1,0 e 1,5 L min-1 e quatro períodos de armazenamento pós-colheita (0; 24; 48 e 72 h. A determinação do teor de nitrato foi realizada por meio do método colorimétrico e os valores obtidos foram inferiores ao limite máximo exigido pela Comunidade Europeia. Os fatores investigados influenciaram significativamente os teores médios de nitrato.The objective of this study was to determine the nitrate (NO3- content present in lettuce plants (Lactuca sativa L. of the Vera cultivar, produced under hydroponic system, in function of different flow rates and post-harvest periods. The experiment was conducted in the city of Cascavel-PR, in a greenhouse of the Universidade Estadual do Oeste do Paraná (UNIOESTE. The plants were nourished by the use of a nutrient solution, characterized by mean electrical conductivity of 1.2 dS m-1 which after passing the entire growing bench, returned to the irrigation tank and restarted the process, forming a closed system. The experiment was conducted in completely randomized design with eight replications, which were represented

  17. O cuidado nos centros municipais educacionais infantis em relação às infecções respiratórias agudas = The care in the municipal centers for children education about acute respiratory infections

    Directory of Open Access Journals (Sweden)

    Eloeth Kaliska Piva

    2012-01-01

    Full Text Available As infecções respiratórias agudas (IRA são causa de morbidade e mortalidade na infância, e sua crescente manifestação e transmissão nos centros educacionais infantis têm sugestionado maior atenção aos cuidados prestados nesses locais as crianças. Este estudo teve por objetivo conhecer as atitudes preventivas e curativas do cuidado proporcionado pelos monitores educacionais dos Centros Municipais Educacionais Infantis da Cidade de Cascavel - PR diante das IRA. Para tanto, realizamos entre novembro a dezembro de 2008, uma pesquisa qualitativa com 12 monitores educacionais, por meio da entrevista semi-estruturada gravada. Os aspectos emergentes das entrevistas foram pontuados e orientado na ótica do cuidado integral de José Ricardo de Carvalho Mesquita Ayres. As ações das monitoras educacionais em relação as IRA têm caráter curativo, sendo fundamentadas no cuidado técnico e entendimentos causais. Nessas ações faltam elementos que compõem o cuidado integral como o movimento, a interação, a reconstrução de identidades e alteridades, a não-causalidade, a plasticidade, a temporalidade e a responsabilidade. Diante disso, enfatiza-se a necessidade de preparar os monitores e as instituições para o cuidado integral. E, sobretudo, a articulação dos centros educacionais aos setores de saúde promovendo a aplicação de estratégias para o controle, prevenção e promoção da saúde das crianças.Acute respiratory infections are the causes of morbidity and mortality during infancy and childhood. Their increasing manifestation and transmission in day-care centers have demanded greater attention for children’ care in such institutions. Current research investigates prevention and care activities provided by educational monitors of day-care center in Cascavel, Paraná State, Brazil, with regard to acute respiratory infections. A qualitative research was undertaken with 12 educational monitors between November and December 2008

  18. A eficiência de um programa de reabilitação audiológica em idosos com presbiacusia e seus familiares The efficiency of an auditory rehabilitation program in elderly people with presbycusis and their family

    Directory of Open Access Journals (Sweden)

    Christine Vieira Ruschel

    2007-06-01

    Full Text Available OBJETIVO: O objetivo dessa pesquisa foi desenvolver um programa de treinamento de estratégias de comunicação para indivíduos idosos, portadores de deficiência auditiva, usuários de aparelhos de amplificação sonora individual e seus familiares. Além disso, objetivamos verificar a contribuição da participação de familiares no processo de reabilitação audiológica desses sujeitos e promover melhor adaptação dos mesmos em relação ao uso do aparelho auditivo. MÉTODOS: Fizeram parte da pesquisa 30 indivíduos com presbiacusia da cidade de Cascavel (PR. A amostra foi divida em dois grupos, o grupo 1 (grupo controle foi constituído por 15 indivíduos que receberam uma única sessão de orientação e o grupo 2 (grupo experimental constituiu-se de 15 idosos que participaram de um programa de reabilitação fonoaudiológica fracionado em seis sessões. RESULTADOS: Foram encontradas diferenças estatisticamente significativas entre os grupos controle e experimental, quando comparadas as entrevistas dos pacientes e familiares, em aspectos como: manuseio da prótese auditiva, uso do telefone com a prótese auditiva e uso do diálogo em ambientes ruidosos. CONCLUSÕES: Neste estudo constatou-se que os idosos tiveram necessidade de acompanhamento fonoaudiológico após a adaptação do aparelho auditivo, pois sozinhos, muitas vezes, não conseguiam manusear a prótese auditiva e utilizá-la de maneira adequada.PURPOSE: The aim of this research consisted on developing a training program of communicative strategies for elderly people with auditory problems, users of individual hearing aids, and their families. Additionally, we had the aim to verify the contribution of family intervention during the process of auditory rehabilitation and to promote better adaptation of these individuals regarding the use of their hearing aids. METHODS: Thirty elderly subjects with presbycusis from the city of Cascavel (PR - Brazil took part in this research

  19. Unidades de manejo a partir de dados de produtividade = Determination of management zones using yield data.

    Directory of Open Access Journals (Sweden)

    Lairton Milani

    2006-10-01

    Full Text Available A agricultura de precisão é um conjunto de tecnologias que visa o aumento da eficiência com base no manejo diferenciado de áreas agrícolas. Neste contexto, é importante estabelecer metodologias nos quais informações de produtividade, solo ou indicadores compostos possam ser utilizados para a determinação de unidades de manejo. O objetivo deste trabalho foi desenvolver uma metodologia para definição de unidades de manejo em função de mapas de produtividade de cinco safras agrícolas. Foi medida a produtividade da cultura soja, durante os anos de 1998 a 2002, de uma área localizada no município de Cascavel, Estado do Paraná, e utilizando técnicas de geoestatística foram gerados mapas de produtividade. A área amostrada possui 1,74 ha, sendo constituída de 256 parcelas, 128 com manejo químico localizado e 128 sem manejo químico localizado. Na colheita, utilizou-se uma colhedora de parcelas e verificou-se dependência espacial para os sistemas de cultivo, em todos os anos. Os valores pontuais de produtividade de soja de cada ano foram padronizadosutilizando-se a técnica denominada escore padrão, em seguida reclassificados em baixo, médio e alto, o que possibilitou a comparação das produtividades de diferentes anos e a geração de um mapa de produtividade média. Com a produtividade padronizada e ocoeficiente de variação classificados em baixo, médio e alto foi gerado um mapa com as unidades de manejo. A metodologia mostrou-se eficiente para identificar regiões homogêneas.Precision agriculture is a set of technologies that aims the efficiency increase based on the differentiated management of agricultural areas. In this context, it is important to establish methodologies to use the yield information, soil or indicators in the determination of management zones. The aim of this paper was to develop a methodology for the definition of management zones according to yield maps of five growing seasons. The soybean yield was

  20. Propriedades físicas de Latossolo Vermelho distroférrico típico sob sistemas de manejo na sucessão soja-milho no período de três anos = Physical properties in distrofic Red Latosol under management systems in the succession soybean-maize in three years

    Directory of Open Access Journals (Sweden)

    Reny Adilmar Prestes Lopes

    2007-12-01

    Full Text Available Este trabalho avaliou alterações das propriedades físicas teor de água, densidade e porosidade do solo em áreas sob sistemas de semeadura direta e preparo convencional nas culturas de soja e milho em três anos agrícolas. O experimento foi instalado no NúcleoExperimental de Engenharia Agrícola da Unioeste (Cascavel, Estado do Paraná. Nos dois primeiros anos foi implantada a cultura de soja; no terceiro ano, milho, como cultura de verão, e aveia preta e nabo forrageiro, como cobertura de inverno. Durante os três anos,foram observados redução do teor de água e densidade do solo e aumento da porosidade. As variações nas propriedades físicas do solo mostraram relação diretamente proporcional entre teor de água e densidade do solo e inversamente para porosidade. O solo apresentoumelhorias nas condições físicas pelo acréscimo de porosidade e redução de densidade com as culturas de aveia preta+nabo forrageiro e milho. O manejo soja/milho em rotação com aveia preta+nabo forrageiro mostrou-se mais adequado na melhoria das condições físicas dosolo do que os sistemas de manejo, uma vez que nenhum dos sistemas teve maior destaque na melhoria das propriedades físicas avaliadas em todo o período.This study evaluated alterations in the physical properties water content, soil density and porosity in areas under no tillage and tillage systems in the cultures of soybean and maize in three agricultural years. The experiment was carried out at the Experimental Nucleus of Agricultural Engineering of Unioeste (Cascavel, state of Paraná. Soybean culture occurred in the first two years and in the third year maize, as summer crop, black oats and forage turnip as winter cover crops. During these three years the study observed reduction of water content and soil density andincrease of porosity . The variations as regards the physical properties of the soil showed direct proportional relation between water content and soil density and was

  1. Ocorrência de Commelina villosa como planta daninha em áreas agrícolas no estado do Paraná-PR, Brasil Occurence of Commelina villosa as weed in Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    Dalva C. Rocha

    2000-01-01

    Full Text Available As trapoerabas pertencem à família Commelinaceae e são plantas daninhas de difícil controle em diferentes regiões do país. No Brasil, a espécie Commelina benghalensis destaca-se como a principal trapoeraba infestante nas culturas de soja, milho, café e citros. Outras duas espécies desse gênero, Commelina diffusa e Commelina erecta, também são conhecidas como infestantes de ocorrência freqüente no território nacional. Commelina villosa está registrada, até o momento, apenas para os Estados da Bahia e Goiás além do Distrito Federal. O presente trabalho teve como objetivos caracterizar e registrar a ocorrência de C. villosa no Estado do Paraná, onde pode estar sendo confundida com outras trapoerabas, principalmente C. benghalensis. Exemplares de C. villosa e C. benghalensis foram coletados, de forma aleatória, em lavouras de soja, feijão e milho, nos municípios de Ponta Grossa, Tibagi, Piraí do Sul, Guarapuava, Pato Branco, Francisco Beltrão, Cascavel, Campo Mourão e Londrina. Parte desse material foi herborizado para a confecção de exsicatas e outra parte foi mantida in vivo, cultivada no Departamento de Botânica, do Instituto de Biociências de Botucatu-UNESP. Caracteres morfológicos descritivos e quantitativos foram avaliados e as espécies comparadas entre si. C. villosa distinguiu-se de C. benghalensis por apresentar folhas maiores (9,76 x 3,26 cm, elíptica a elípticaestreita, sésseis, de coloração verde escura com manchas violáceas na face inferior, filetes translúcidos, entre outras características. A ocorrência de C. villosa no Paraná foi constatada em todos os municípios amostrados, com exceção de Campo Mourão e Londrina.Commelinaceae are weeds with unsatisfactory control in different parts of the country. Commelina benghalensis is the main Commelinaceae weed in soybean, corn, coffee and orange crops in Brazil. Commelina diffusa and Commelina erecta are also known as common weeds in national

  2. Quantitative genetic analysis of methylxanthines and phenolic compounds in mate progenies Análise genética quantitativa de metilxantinas e compostos fenólicos em progênies de erva-mate

    Directory of Open Access Journals (Sweden)

    Euclides Lara Cardozo Junior

    2010-02-01

    Full Text Available The objective of this work was to determine the contents of methylxanthines, caffeine and theobromine, and phenolic compounds, chlorogenic and caffeic acids, in 51 mate progenies (half-sib families and estimate the heritability of genetic parameters. Mate progenies were from five Brazilian municipalities: Pinhão, Ivaí, Barão de Cotegipe, Quedas do Iguaçu, and Cascavel. The progenies were grown in the Ivaí locality. The contents of the compounds were obtained by high performance liquid chromatography (HPLC. The estimation of genetic parameters by the restricted maximum likelihood (REML and the prediction of genotypic values via best linear unbiased prediction (BLUP were obtained by the Selegen - REML/BLUP software. Caffeine (0.248-1.663% and theobromine (0.106-0.807% contents were significantly different (p0.5. The two different progeny groups determined for chlorogenic (1.365-2.281% and caffeic (0.027-0.037% acid contents were not significantly different (pO objetivo deste trabalho foi determinar o teor de metilxantinas, cafeína e teobromina, e de compostos fenólicos, ácido clorogênico e ácido cafeico, em 51 progênies de erva-mate e estimar componentes de variância e herdabilidade. As progênies de erva-mate eram oriundas de cinco municipios brasileiros: Pinhão, Ivaí, Barão do Cotegipe, Quedas do Iguaçu e Cascavel. Essas progênies foram cultivadas na localidade de Ivaí. O conteúdo dos compostos foi obtido por cromatografia líquida de alta eficiência (CLAE. Na estimativa dos componentes da variância e dos parâmetros genotípicos, utilizou-se a metodologia de modelos mistos para a obtenção da melhor predição linear não viciada (BLUP dos efeitos genotípicos e o processo da máxima verossimilhança restrita (REML, processados pelo programa Selegen - REML/BLUP. Os conteúdos de cafeína (0,248-1,663 % e teobromina (0,106-0.807% foram significativamente (p0,5. Foram determinados dois diferentes grupos de progênie para

  3. Efeito do armazenamento à baixa temperatura (-4 ºC na cor e no teor de acidez da farinha de trigo Effect of storage at low temperature (-4 ºC in the color and acidity content of wheat flour

    Directory of Open Access Journals (Sweden)

    Fernanda Ortolan

    2010-03-01

    Full Text Available Durante o armazenamento de farinha de trigo, podem ocorrer mudanças bioquímicas que resultam em alterações nutricionais e tecnológicas, sendo que em baixa temperatura estas mudanças ocorrem mais lentamente. Entre as alterações, pode-se citar o aumento da acidez, a redução do pH e as modificações na sua cor. O presente trabalho teve por objetivo avaliar os efeitos do armazenamento à baixa temperatura (-4 ºC na cor e na acidez da farinha de trigo durante seis meses e correlacionar os resultados obtidos para investigar se a acidez tem relação com a cor da farinha. Foram utilizadas farinhas provenientes de dez genótipos de trigo cultivados em Cascavel, no Estado do Paraná. A cor foi determinada no sistema CIEL*a*b* através dos parâmetros de cor: L* (luminosidade, a* e b* (coordenadas de cromaticidade. Usou-se procedimento da AOAC para determinação da acidez de lipídios. As farinhas apresentaram tendência ao branqueamento durante o período de seis meses de armazenamento, com ocorrência de leve aumento da acidez, mas dentro dos teores aceitáveis. A acidez não foi considerada um fator envolvido na alteração da cor das farinhas no armazenamento à baixa temperatura, pois não houve correlação entre estes dois parâmetros.During wheat flour storage, biochemical changes can occur which lead to nutritional and technological alterations, but in low temperature these changes can occur more slowly. Among the reactions, we may note are an increase in acidity, reduction of pH and modifications to its color. The present work aimed to evaluate the effects of storage at low temperature (-4 ºC on the color and acidity of wheat flour over six months and to correlate the results obtained to discover whether the acidity has any relationship with the color of the flour. Flours of ten wheat genotypes, cultivated in Cascavel, in Paraná State were used. The color was determined via the CIEL*a*b* system using the color parameters: L

  4. Inoculation with Azospirillum, associated with nitrogen fertilization in maize

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Lana

    2012-06-01

    Full Text Available The biological nitrogen fixation is an alternative to supply the nitrogen needed for maize. The objective of this study was to evaluate the development and yield of maize in response to inoculation with Azospirillum associated with nitrogen fertilization. We conducted two field experiments in the summer harvest, the first in the 2000/2001 crop year in the region of Marechal Cândido Rondon, under conventional tillage, and second in the 2002/2003 agricultural year in the region of Cascavel, under no tillage. The experimental design in both experiments was a randomized complete block, with four replications, 2x2x2 factorial, with two levels of nitrogen at sowing (zero and 20 kg ha-1, two levels of inoculum (zero and 200 g ha-1 and two levels of nitrogen in topdressing (zero and 100 kg ha-1. There was evaluated the height of ear insertion, total plant height, leaf N content, shoot dry biomass and grain yield. The height of ear insertion and total plant height were not influenced by the factors under study. Nitrogen fertilization at sowing increased the leaf N content, causing the opposite effect when combined with inoculation. Inoculation with Azospirillum in the absence of nitrogen, provide productivity increases of 15.4% and 7.4% for 2000/2001 and 2002/2003 crops, respectively. The inoculation provided productivity similar to that obtained with 100 kg ha-1 in topdressing in crop 2000/2001, while in association with the topdressing, reduced productivity and shoot dry biomass in crop 2002/2003.

  5. Prevalence of serological markers of hepatitis B virus in pregnant women from Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    D.A. Bertolini

    2006-08-01

    Full Text Available The prevalence of hepatitis B virus (HBV in Brazil increases from South to North but moderate to elevated prevalence has been detected in the Southwest of Paraná State. The prevalence of serological markers of HBV was evaluated in 3188 pregnant women from different counties in Paraná State and relevant epidemiological features were described. The prevalence of HBV markers in pregnant women for the state as a whole was 18.5% (95% CI = 17.2-19.9, ranging from 7.2% in Curitiba to 38.5% in Francisco Beltrão. The endemicity of HBV marker prevalence in pregnant women was intermediate in Cascavel, Foz do Iguaçu, and Francisco Beltrão, and low in Curitiba, Londrina, Maringá, and Paranaguá. Multiple logistic regression showed that HBV marker prevalence increased with age, was higher among black women, among women of Italian and German descent, and among women who had family members in neighboring Rio Grande do Sul State. Univariate analysis showed that HBV marker prevalence was also higher among women with no education or only primary education, with a lower family income and whose families originated from the South Region of Brazil. Pregnant women not having positive HBV markers (anti-HBc, HBsAg or anti-HBs detected by ELISA corresponded to 73.7% of the population studied, implying that HBV vaccination needs to be reinforced in Paraná State. The highest prevalence was found in three counties that received the largest number of families from Santa Catarina and Rio Grande do Sul, where most immigrants were of German or Italian ascendance. This finding probably indicates that immigrants that came to this area brought HBV infection to Southwestern Paraná State.

  6. Estudo retrospectivo das complicações intra-operatórias na cirurgia de revascularização do miocárdio

    Directory of Open Access Journals (Sweden)

    Ariana Rodrigues Silva Carvalho

    2008-12-01

    Full Text Available As complicações intra-operatórias na cirurgia de revascularização miocárdica (CRM quando reconhecidas previamente podem guiar os cuidados prestados na sala operatória, dando continuidade na Unidade de Terapia Intensiva e durante toda internação. Quando a equipe conhece essas complicações sofridas pelo indivíduo poderá prever e prover cuidados mais específicos na seqüência de sua evolução cirúrgica. O objetivo desse estudo foi investigar a freqüência e tipos de complicações observadas no intra-operatório da CRM. Trata-se de um estudo retrospectivo, descritivo, transversal, baseado em dados secundários, com amostra intencional de 123 sujeitos. A coleta de dados realizou-se por meio do acesso ao banco de dados do Instituto de Moléstias Cardiovasculares do Oeste do Paraná, em Cascavel. Os dados foram analisados no programa computadorizado Statistic, versão 6,0. Os tipos de complicações observadas nesse período foram: cardiovasculares, pulmonares, alteração nos vasos do enxerto e sangramento. Dentre essas, a que obteve maior freqüência foi “alteração nos vasos do enxerto”, em 33 (26,8% casos. As complicações no intra-operatório podem não ser passíveis de prevenção quando consideradas as características individuais de cada sujeito, entretanto, todos os esforços precisam ser direcionados para que o evento, quando ocorrer, seja bem atendido, evitando dessa forma, complicações adicionais maiores.

  7. Rede e apoio social familiar no seguimento do recém-nascido pré-termo e baixo peso ao nascer

    Directory of Open Access Journals (Sweden)

    Débora Falleiros de Mello

    2010-03-01

    Full Text Available Um dos aspectos ligados à sobrevida do recém-nascido (RN pré-termo (PT e de baixo peso ao nascer (BPN é relativo ao período após a alta hospitalar. Este estudo tem por objetivo identificar a rede social e o apoio social de famílias com filho PT e BPN egresso da UTI neonatal, a partir da utilização dos instrumentos genograma e ecomapa com estas famílias no município de Cascavel-Paraná. É um estudo descritivo, com abordagem qualitativa, sendo que a partir dos relatos de seis famílias com filhos egressos de UTIN, acompanhadas por meio de visitas domiciliares nos três primeiros meses após a alta hospitalar, obtiveram-se dados que foram agrupados ao redor de três temas: A rede social como integrante do seguimento do PT e BPN no contexto da família; O apoio social nas experiências das famílias com bebê PT e BPN; A vulnerabilidade e o contexto da rede e do apoio social. Emergiu no estudo, um conjunto de atores e cenários que fazem parte da rede ligada às famílias no seu cotidiano. A atuação da enfermagem ganha mais sentido com o conhecimento dessa rede, que proporciona uma integração entre saberes práticos e saberes técnicos.

  8. terapêutica

    Directory of Open Access Journals (Sweden)

    Marisa Ines Tomazzoni

    2006-01-01

    Full Text Available Se trata de un estudio etnobotánico e investigación de opinión, que tuvo como objetivo ampliar el conocimiento sobre el uso de las plantas medicinales en la comunidad de la Municipalidad de Cascavel-PR, con miras a apoyar en la implantación de los fitoterápicos en la red pública de la salud. La muestra correspondió a 50 familias del área de la jurisdicción de una Unidad Básica de Salud, seleccionadas mediante una muestra estratificada proporcional. Para la obtención de los datos se optó por una investigación de carácter exploratório-descriptivo, a través de la entrevista semiestructurada. Como resultado, se observo que el 96% de la población albo manifestó el uso de la plantas; fueron registradas 271 referencias etnobotánicas, englobando 75 etnoespecies; 40 propiedades terapéuticas estaban relacionadas al uso doméstico. El uso de las plantas es bastante difundido y la transferencia eel conocimiento etnobotánico sigue los padrones de las comunidades trradicionales, no existiendo bloqueos en éste proceso. Se constató también, que las plantas medicinales para la comunidad representan un factor importante para la manutención de las condiciones de la salud, formando parte de un saber local preservado.

  9. AVALIAÇÃO E COMPARAÇÃO ENTRE KITS LABORATORIAIS “D-DÍMERO” COMO DIAGNÓSTICO DE TROMBOSE VENOSA PROFUNDA

    Directory of Open Access Journals (Sweden)

    Rafaela Natiéli LIMA

    2015-12-01

    Full Text Available O presente estudo teve como objetivos realizar análise comparativa entre kits laboratoriais D-Dímero para Trombose Venosa Profunda (TVP pelos métodos de Imunoturbidimetria (metodologia 1 e Quimiluminescência (metodologia 2, ambos da marca Siemens, e comparar o resultado das análises realizadas, com o diagnóstico clínico confirmatório do paciente, analisando seus prontuários. A amostra foi constituída por uma população de 95 voluntários que realizaram o teste laboratorial D-Dímero, em um laboratório de análises clínicas da cidade de Cascavel-PR. Os resultados foram comparados através dos parâmetros para validação sorológica, através dos cálculos de especificidade, o qual foi de 33% em ambos os métodos, sensibilidade, o qual foi de 89% e 90%, e a eficiência de 68% e 69%, respectivamente para as metodologias 1 e 2. O valor preditivo positivo (VPP foi de 69% em ambos os testes, o valor preditivo negativo (VPN foi de 33%, a prevalência foi de 62%, e a prevalência sorológica foi de 81%. Conclui-se assim que, os testes analisados são pouco específicos para TVP, não podendo ser utilizados como método diagnóstico, mas sim para excluir prováveis suspeitas de tromboembolismo venoso.

  10. Frequency of potential interactions between drugs in medical prescriptions in a city in southern Brazil

    Directory of Open Access Journals (Sweden)

    Genici Weyh Bleich

    Full Text Available CONTEXT AND OBJECTIVE: Drug interactions form part of current clinical practice and they affect between 3 and 5% of polypharmacy patients. The aim of this study was to identify the frequency of potential drug-drug interactions in prescriptions for adult and elderly patients. TYPE OF STUDY AND SETTING: Cross-sectional pharmacoepidemiological survey in the Parque Verde housing project, municipality of Cascavel, Paraná, Brazil, between December 2006 and February 2007. METHODS: Stratified cluster sampling, proportional to the total number of homes in the housing project, was used. The sample consisted of 95 homes and 96 male or female patients aged 19 or over, with medical prescriptions for at least two pharmaceutical drugs. Interactions were identified using DrugDigest, Medscape and Micromedex softwares. RESULTS: Most of the patients were female (69.8%, married (59.4% and in the age group of 60 years or over (56.3%, with an income less than or equal to three minimum monthly salaries (81.3% and less than eight years of schooling (69.8%; 90.6% of the patients were living with another person. The total number of pharmaceutical drugs was 406 (average of 4.2 medications per patient. The drugs most prescribed were antihypertensives (47.5%. The frequency of drug interactions was 66.6%. Among the 154 potential drug interactions, 4.6% were classified as major, 65.6% as moderate and 20.1% as minor. CONCLUSION: The high frequency of drug prescriptions with a potential for differentiated interactions indicates a situation that has so far been little explored, albeit a reality in household surveys.

  11. Association between cardiorespiratory fitness and body fat in girls

    Science.gov (United States)

    Minatto, Giseli; de Sousa, Thiago Ferreira; de Carvalho, Wellington Roberto Gomes; Ribeiro, Roberto Régis; Santos, Keila Donassolo; Petroski, Edio Luiz

    2016-01-01

    Abstract Objective: To estimate the prevalence of low cardiorespiratory fitness and its association with excess body fat, considering the sexual maturation and economic level in female adolescents. Methods: Cross-sectional, epidemiological study of 1223 adolescents (10-17 years) from the public school system of Cascavel, PR, Brazil, in 2006. We analyzed the self-assessed sexual maturation level (prepubertal, pubertal and post-pubertal), the economic level (high and low) through a questionnaire and body fat (normal and high) through triceps and subscapular skinfolds. The 20-meter back-and-forth test was applied to estimate maximum oxygen consumption. Cardiorespiratory fitness was assessed according to reference criteria and considered low when the minimum health criterion for age and sex was not met. Chi-square test and logistic regression were applied, with a significance level of 5%. Results: The prevalence of low cardiorespiratory fitness was 51.3%, being associated with all study variables (p<0.001). At the crude analysis, adolescents with high body fat were associated with low cardiorespiratory fitness, when compared to those with normal body fat (OR=2.76; 95%CI: 2.17-3.52). After adjustment by sexual maturation, this association remained valid and showed an effect that was 1.8-fold higher (95%CI: 1.39-2.46) and after adjusting by economic level, the effect was 1.9-fold higher (95%CI: 1.45-2.61). Conclusions: Approximately half of the assessed girls showed unsatisfactory levels of cardiorespiratory fitness for health, which was associated with high body fat, regardless of sexual maturation level and economic level. Effective public health measures are needed, with particular attention to high-risk groups. PMID:27131896

  12. Energy cost simulations for heating in greenhouses for five cities in Brazil; Simulacoes do custo de energia para calefacao em casas de vegetacao para cinco cidades brasileiras

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Celso Eduardo Lins de [Universidade Estadual do Oeste do Parana (UNIOESTE), Cascavel, PR (Brazil)], e-mail: celsooli@unioeste.br; La Plaza, Saturnino de; Garcia, Jose Luis [Universidad Politecnica de Madrid (UPM), Madrid, (Spain). Escuela Tecnica Superior de Ingenieros Agronomos

    2004-07-01

    Using the values of the global heat loss coefficient (U) calculated with the equations of the lineal model developed by OLIVEIRA (2003), and with the 30 year-old values, the energy can be calculated by square meter that would be necessary to obtain a temperature set point of 15 deg C, to a greenhouse of 132 m{sup 2}. They were chosen the cities of Cascavel, Curitiba, Florianopolis, Porto Alegre and Sao Paulo. To each place the medium day of the months was used to determine the convenience of the heating that the minimum average went under 15 deg C. Observing the medium minimum temperatures is easy to verify that the support of alone heat would be justified for a crop with high energy need and excellent commercial value, as it can be the case of the crop of some ornamental species. The value of estimated energy was multiplied by the Euros cost for Kwh for fuel-oil, natural gas and diesel, being simulate the heating use for heat fan and radiant floor, combined or not with two techniques of energy saving: use a double layer or plastic tunnel over the crop. Should even consider that in all the chosen places frost risk exists, what can influence in the profitability of many crops. The city of Florianopolis went to that presented smaller thermal solicitations and, therefore smaller costs, the city of Curitiba already demonstrated the needs of a support system for eight months and, therefore, larger costs. The technical more efficient it is the use of combined radiant floor with plastic tunnel, being the combustible more economic is fuel-oil. (author)

  13. Methodology for quantifying the technological level of energy in electric motors; Metodologia para quantificacao do nivel tecnologico energetico de motores eletricos

    Energy Technology Data Exchange (ETDEWEB)

    Konopatzki, Evandro Andre; Bianchessi, Joel Marcos [Faculdade Assis Gurgacz (FAG), Cascavel, PR (Brazil); Oliveira, Celso Eduardo Lins de [Universidade de Sao Paulo (FZEA-USP), Pirassununga, SP (Brazil). Fac. de Zootecnia e Engenharia de Alimentos; Konopatzki, Angelica da Silva Lima [Faculdade de Ciencias Aplicadas de Cascavel (FACIAP), PR (Brazil). Curso de Ciencia da Computacao; Marim, Cesar [Universidade Estadual do Oeste do Parana (UNIOESTE), Cascavel, PR (Brazil)

    2006-07-01

    The market competition created in agricultural industry because of its insertion in the domestic and foreign markets, verified by the increase in GDP share of agribusiness in the Brazilian GDP, led us to have better control of costs and inputs. Thus the final value of products sold by these agribusinesses can download increasing the space in the consumer market. Electricity is one of the inputs now being studied and this paper presents a model for determining the coefficients that allow the comparison of different existing technologies for electric motors inserting an indicator of technological innovation called Technological Level Energy (NTE). The NTE was developed and tested in Storage Units of Grain (UAG) in the region of Cascavel - Parana where data were collected in six UAG, analyzed in medium weighted, had levels of different energy technology development. The main elements used to create the NTE are the number of engines installed in UAG, the lifetime of the engine considered from its manufacture and motor plate data such as income and the power factor. As a comparative process, each region can create your NTE and analyze your situation in a national context. The UAG will use the NTE to technological innovation and planning projects such as depreciation, financing and cross-examination of financial resources for the exchange of electric motors. Thus the NTE will aid in comparative indication of the timing for replacement of electric motors. Moment from which the energy demand, according to the obsolescence of equipment, are considered excessive compared to other more modern UAG. (author)

  14. Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela: Contributing to its taxonomy and snakebite management.

    Science.gov (United States)

    Calvete, Juan J; Borges, Adolfo; Segura, Alvaro; Flores-Díaz, Marietta; Alape-Girón, Alberto; Gutiérrez, José María; Diez, Nardy; De Sousa, Leonardo; Kiriakos, Demetrio; Sánchez, Eladio; Faks, José G; Escolano, José; Sanz, Libia

    2009-03-06

    The taxonomic status of the medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela, which has been classified as Bothrops colombiensis, remains incertae cedis. To help resolving this question, the venom proteome of B. colombiensis was characterized by reverse-phase HPLC fractionation followed by analysis of each chromatographic fraction by SDS-PAGE, N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. The venom contained proteins belonging to 8 types of families. PI Zn(2+)-metalloproteinases and K49 PLA(2) molecules comprise over 65% of the venom proteins. Other venom protein families comprised PIII Zn(2+)-metalloproteinases (11.3%), D49 PLA(2)s (10.2%), l-amino acid oxidase (5.7%), the medium-sized disintegrin colombistatin (5.6%), serine proteinases (1%), bradykinin-potentiating peptides (0.8%), a DC-fragment (0.5%), and a CRISP protein (0.1%). A comparison of the venom proteomes of B. colombiensis and B. atrox did not support the suggested synonymy between these two species. The closest homologues to B. colombiensis venom proteins appeared to be toxins from B. asper. A rough estimation of the similarity between the venoms of B. colombiensis and B. asper indicated that these species share approximately 65-70% of their venom proteomes. The close kinship of B. colombiensis and B. asper points at the ancestor of B. colombiensis as the founding Central American B. asper ancestor. This finding may be relevant for reconstructing the natural history and cladogenesis of Bothrops. Further, the virtually indistinguishable immunological crossreactivity of a Venezuelan ABC antiserum (raised against a mixture of B. colombiensis and Crotalus durissus cumanensis venoms) and the Costa Rican ICP polyvalent antivenom (generated against a mixture of B. asper, Crotalus simus, and Lachesis stenophrys venoms) towards the venoms of B. colombiensis and B. asper, supports this

  15. Methemoglobinemia associated with loxoscelism Meta-hemoglobinemia associada ao loxoscelismo

    Directory of Open Access Journals (Sweden)

    Orlando C. de O. Barreto

    1990-02-01

    Full Text Available In twenty five patients who presented the cutaneous form of loxoscelism, serum haptoglobin and lactic dehydrogenase, erythrocyte glucose-6-phosphate dehydrogenase, glutathione reductase, glutathione peroxidase, methemoglobin, bilirubin and reticulocytes were investigated after bite. No hemolysis was detected but an increase in methemoglobin was found in 54% of the cases; in 7% it was between 1.1% and 2%, in 27% it ranged from 2.1% to 4%, and in 20% from 4.1% to 8%. Blood samples of a normal, blood group 0 individual and of a patient who exhibited methemoglobinemia after Loxosceles bite were incubated separately with antisera against Loxosceles gaucho, Crotalus terrificus, Bothrops jararaca, with Loxosceles gaucho venom and 0.3% phenol. No methemoglobin was found after 1, 4,8 and 15 days in both sets of samples. At the 25th day all the samples, including the controls, exhibited similar methemoglobin reductase decrease. The data suggest that the methemoglobinemia which occurs in 50% of the patients probably arises from in vivo venom metabolism, inasmuch as the crude venom does not induce methemoglobinemia.Vinte e cinco pacientes que apresentaram a forma cutânea do loxoscelismo foram estudados após a picada, determinando-se a glicose-6-fosfato desidrogenase, glutationa redutase e glutationa peroxidase eritrocitárias, haptoglobina e latico desidrogenase séricas, bilirrubina, reticulócitos e meta-hemoglobina. Não foi observada hemólise aumentada, mas foi detectado aumento da meta-hemoglobina em 54% dos casos: em 7% entre 1,1% e 2%, em 27% variou de 2,1% a 4%, e em 20% de 4,1 a 8%. Amostras de sangue de um indivíduo normal do grupo 0 de uma paciente que exibiu meta-hemoglobina após picada por Loxosceles foram incubadas separadamente com anti-soros contra Loxosceles gancho, Crotalus terrificus e Bothrops jararaca, com veneno de Loxosceles gaucho e fenol a 3%, e não se detectou aumento de meta-hemoglobina depois de 1, 4, 8 e 15 dias em todas

  16. Novo método de dosagem de soros antipeçonhentos em camundongos lactentes: I - dosagem do soro anticrotálico New method for dosage of antivenoms sera in baby mice: I-Anticrotalic serum's dosage

    Directory of Open Access Journals (Sweden)

    Bruno Soerensen

    1984-08-01

    Full Text Available A atividade biológica dos soros antipeçonhentos pode ser determinada "in vivo" em pombos, coelhos, cobaios e camundongos adultos, não existindo, entretanto, nenhum método que possa ser recomendado internacionalmente. Estudou-se comparativamente aos métodos tradicionais de dosagem de soros em pombos e camundongos adultos, a validade do uso de camundongos lactentes de 6 a 7 dias, pesando 4 a 5g, inoculados pela via subcutânea. Inicialmente foi determinada a toxidez do veneno de Crotalus durissus terríficus através do estudo da sintomatologia do envenenamento e da atividade letal. O estudo comparativo dos três métodos forneceu maior concordância de resultados em DE100 entre as dosagens realizadas com camundongos lactentes e adultos. A DE100 e DL50 determinadas em camundongos lactentes forneceu resultados mais constantes que os dos métodos de camundongos adultos e pombos. O uso deste novo método permite eliminar a dificuldade de obtenção do atual animal de prova; a utilização de um maior número de animais por ponto de avaliação biológica, possibilitando uma maior precisão e conseguindo-se uma uniformidade nas características exigidas neste tipo de dosagem como peso, idade e linhagem, visando a reprodução sistemática dos resultados.The biological activity of antiserum against venoms can be assessed by "in vivo" tests using pigeons, rabbits, guinea-pigs and adult mice although there is no International standardized reaction for it. The traditional methods using pigeons and adult mice were comparatively studied with those using mice of 4-5 g (6-7 days of age, inoculated by subcutaneous injection. The toxicity of the Crotalus durissus terrificus venom was initially determined by studying the symptomatology after administration of venom and lethal activity. The comparative study of these three methods showed that the best concordance in the results of the ED100 was obtained using adult and baby mice. The most homogeneous ED100

  17. Influenza em animais heterotérmicos Influenza in heterothermics

    Directory of Open Access Journals (Sweden)

    Dalva Assunção Portari Mancini

    2004-06-01

    Full Text Available O objetivo foi pesquisar Ortomyxovirus em animais heterotérmicos. Coletou-se sangue de serpentes dos gêneros Bothrops e Crotalus e de sapo e rãs dos gêneros Bufo e Rana, para a detecção dos receptores de hemácias e anticorpos específicos, ao vírus influenza, pelos testes de hemaglutinação e inibição da hemaglutinação, respectivamente. Pelo teste de hemaglutinação, verificou-se que serpentes e sapos em cativeiro apresentaram receptores em suas hemácias para o vírus influenza, humano e eqüino do tipo A e tipo B. O mesmo ocorreu com serpentes recém chegadas. Quanto ao teste de inibição da hemaglutinação dos soros dos répteis observou-se títulos protetores de anticorpos aos vírus influenza tipo A (origens humana e eqüina e tipo B. Com soro de sapo não se observou reação de inibição da hemaglutinação porém, 83,3% das rãs obtiveram médias de 40UIH para algumas cepas. Conclui-se que animais heterotérmicos podem oferecer condições de hospedeiros aos vírus influenza, assim como susceptibilidade à infecção.The objective was to study Orthomyxovirus in heterothermic animals. Blood samples from snakes (genus Bothrops and Crotalus and from toads and frogs (genus Bufo and Rana were collected to evaluate the red cell receptors and antibodies specific to influenza virus by the hemagglutination and hemagglutination inhibition tests, respectively. Both snakes and toads kept in captivity presented receptors in their red cells and antibodies specific to either influenza virus type A (human and equine origin or influenza type B. The same was observed with recently captured snakes. Concerning the influenza hemagglutination inhibition antibodies protective levels were observed in the reptiles' serum, against influenza type A and type B. Unlike the toads, 83.3% of the frogs presented mean levels of Ab 40HIU for some influenza strains. It was concluded that heterothermic animals could offer host conditions to the influenza

  18. Reactividad inmunoquímica de sueros anti- Caiman yacare y Caiman latirostris frente a sueros de diferentes especies

    Directory of Open Access Journals (Sweden)

    de Roodt, Adolfo Rafael

    2010-06-01

    Full Text Available Se estudió la reactividad inmunoquímica entre los sueros de distintas especies de reptiles frente a sueros hiperinmunes experimentales anti-suero de Caiman yacare y anti-suero de Caiman latirostris. Los sueros que se probaron fueron los homólogos de Caiman yacare, Caiman latirostris y los heterólogos de Alligator missisipiensis, Tupinambis merinae, Tupinambis rufescens, Chelonoidis chilensis, Clelia rustica, Waglerophis merremii, Lystrophys dorbignyi, Phyton molurus, Boa constrictor occidentalis, Eunectes notaeus, Crotalus durissus terrificus, Bothrops alternatus, Bothrops diporus, Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni, Pitangus sulphuratus y Gallus gallus. La reactividad inmunoquímica se determinó mediante las técnicas de doble inmunodifusión y ELISA, mostrándose importante entre los sueros de los crocodrílidos y baja entre estos y los de las otras especies de reptiles estudiadas. Se observó mayor reactividad entre los antisueros anti-Caiman respecto a los sueros de Caiman latirostris y Caiman yacare que frente al suero de Alligator missisipiensis. Además, se encontró una fuerte reactividad entre ambos sueros anti-Caiman y el de Gallus gallus poniendo en evidencia la fuerte reactividad entre los sueros de arcosaurios. In order to study the immunochemical reactivity among sera from different species of reptiles regarding sera from Caiman, the immunoreactivity of sera from reptiles against antisera to Caiman yacare or anti-Caiman latirostris sera was studied. These hiperimmune sera were tested against sera from Alligator missisipiensis, Tupinambis merinae, Tupinambis rufescens, Chelonoidis chilensis, Clelia rustica, Waglerophis merremii, Lystrophys dorbignyi, Phyton molurus, Boa constrictor occidentalis, Eunectes notaeus, Crotalus durissus terrificus, Bothrops alternatus, Bothrops neuwiedii, Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni, Pitangus sulphuratus and Gallus gallus. The immunochemical

  19. Evolutionary morphology of the rattlesnake style

    Directory of Open Access Journals (Sweden)

    Pires-daSilva André

    2009-02-01

    Full Text Available Abstract Background The rattlesnake rattling system is an evolutionary novelty that includes anatomical, behavioral, and physiological modifications of the generalized pitviper tail. One such modification, the formation of a bony clublike style at the terminal region of the caudal vertebrae, has not previously been examined in a phylogenetic context. Here we used skeletal material, cleared and stained preparations, and radiographs of whole preserved specimens to examine interspecific variation in style morphology among 34 rattlesnake species. Results Evolutionary Principal Components Analysis revealed an inverse relationship between caudal segmental counts and style size, supporting the hypothesis that bone from caudal vertebral elements was reallocated to style formation during the evolution of this structure. Most of the basal rattlesnake species have small styles consisting of few compacted vertebral elements; however, early in the rattlesnake radiation there appears to have been two independent transitions to relatively large, pronged styles consisting of multiple coalesced vertebrae (once in Sistrurus catenatus, and once in Crotalus following the divergence of the Mexican long-tailed rattlesnakes. In terms of style shape, the two most divergent species, C. catalinensis and C. ericsmithi, provide insight into the possible relationship between style and rattle matrix morphology and lineage-specific evolutionary strategies for retaining rattle segments. Conclusion The considerable interspecific variation in rattle morphology appears to correspond to variation in the bony style. We hypothesize that style morphology evolves indirectly as an integrated module responding to adaptive evolution on matrix morphology.

  20. Predation and the maintenance of color polymorphism in a habitat specialist squamate.

    Directory of Open Access Journals (Sweden)

    Vincent R Farallo

    Full Text Available Multiple studies have addressed the mechanisms maintaining polymorphism within a population. However, several examples exist where species inhabiting diverse habitats exhibit local population-specific polymorphism. Numerous explanations have been proposed for the maintenance of geographic variation in color patterns. For example, spatial variation in patterns of selection or limited gene flow can cause entire populations to become fixed for a single morph, resulting in separate populations of the same species exhibiting separate and distinct color morphs. The mottled rock rattlesnake (Crotalus lepidus lepidus is a montane species that exhibits among-population color polymorphism that correlates with substrate color. Habitat substrate in the eastern part of its range is composed primarily of light colored limestone and snakes have light dorsal coloration, whereas in the western region the substrate is primarily dark and snakes exhibit dark dorsal coloration. We hypothesized that predation on high contrast color and blotched patterns maintain these distinct color morphs. To test this we performed a predation experiment in the wild by deploying model snakes at 12 sites evenly distributed within each of the two regions where the different morphs are found. We employed a 2×2 factorial design that included two color and two blotched treatments. Our results showed that models contrasting with substrate coloration suffered significantly more avian attacks relative to models mimicking substrates. Predation attempts on blotched models were similar in each substrate type. These results support the hypothesis that color pattern is maintained by selective predation.

  1. A rare case of multiple rattlesnake bites.

    Science.gov (United States)

    Iliev, Yanko T; Kristeva, Sasha A; Prancheva, Maria G

    2013-01-01

    The rattlesnake (Crotalus adamanteus) is a venomous viper inhabiting the southeastern parts of the United States. It is not found in the Balkans and Europe habitats. Subjects of the species are grown and seen in museums, exhibitions and terrariums, and sometimes in private collections. This may generate potentially toxic exposures to the venom in accidental contact. Acute poisoning with rattlesnake poison in Bulgaria is exotic, rare and even casuistic. The venom of the rattlesnake exhibits neuropathic, proteolytic and hemolytic activities. Antivenom is not currently easily available in Bulgaria--it is not usually stored in hospitals because it is very rarely used and therefore rather expensive. We present a case of multiple envenomation (two different occasions) of one and the same person who kept rattlesnakes in a private terrarium. Local toxic syndrome was observed with burning and stinging pain at bite site combined with limited hemorrhage and necrosis. The hemolytic reaction and the local toxic results were successfully managed without resorting to any specific antidotal therapy.

  2. Identification of linear B-cell epitopes on myotoxin II, a Lys49 phospholipase A₂ homologue from Bothrops asper snake venom.

    Science.gov (United States)

    Lomonte, Bruno

    2012-10-01

    Knowledge on toxin immunogenicity at the molecular level can provide valuable information for the improvement of antivenoms, as well as for understanding toxin structure-function relationships. The aims of this study are two-fold: first, to identify the linear B-cell epitopes of myotoxin II from Bothrops asper snake venom, a Lys49 phospholipase A₂ homologue; and second, to use antibodies specifically directed against an epitope having functional relevance in its toxicity, to probe the dimeric assembly mode of this protein in solution. Linear B-cell epitopes were identified using a library of overlapping synthetic peptides spanning its complete sequence. Epitopes recognized by a rabbit antiserum to purified myotoxin II, and by three batches of a polyvalent (Crotalidae) therapeutic antivenom (prepared in horses immunized with a mixture of B. asper, Crotalus simus, and Lachesis stenophrys venoms) were mapped using an enzyme-immunoassay based on the capture of biotinylated peptides by immobilized streptavidin. Some of the epitopes identified were shared between the two species, whereas others were unique. Differences in epitope recognition were observed not only between the two species, but also within the three batches of equine antivenom. Epitope V, located at the C-terminal region of this protein, is known to be relevant for toxicity and neutralization. Affinity-purified rabbit antibodies specific for this site were able to immunoprecipitate myotoxin II, suggesting that the two copies of epitope V are simultaneously available to antibody binding, which would be compatible with the mode of dimerization known as "conventional" dimer.

  3. [The efficacy of the bothropic-crotalic antivenom in the neutralization of the main Bothrops jararacussu venom effects].

    Science.gov (United States)

    dos-Santos, M C; Gonçalves, L R; Fortes-Dias, C L; Cury, Y; Gutiérrez, J M; Furtado, M de F

    1992-01-01

    Myonecrosis is one of the effects of Bothrops jararacussu venom, from which a myotoxin was isolated showing structural homology to phospholipase A2 (PLA2), but without enzymatic activity. Such myotoxic activity is also present in the Crotalus durissus terrifucus venom, and is attributed to crotoxin and to PLA2 (crotoxin B), the basic component of the crotoxin complex. The Bothrops jararacussu venom showed three proteins with immunologic identity to PLA2 from crotoxin. The bothropic (AB) and the bothropic/crotalic (AB/C) anti-venoms, two commercial polyvalent anti-venoms produced at Instituto Butantan, were compared in order to assess their capacity for neutralization of the lethal, hemorrhagic, coagulant and myotoxic activities of Bothrops jararacussu venom. Both anti-venoms showed the same level of hemorrhagic activity neutralization. However, AB/C was about three times more efficient than AB in neutralizing the myotoxic activity, and two times more potent for neutralization of lethality and coagulant activity of Bothrops jararacussu venom. These data suggest that the use of AB/C could be of value in the treatment of patients bitten by snakes of this species.

  4. Parasitological and immunological diagnoses from feces of captive-bred snakes at Vital Brazil Institute.

    Science.gov (United States)

    Souza, Janaína Lima de; Barbosa, Alynne da Silva; Vazon, Adriana Prado; Uchôa, Claudia Maria Antunes; Nunes, Beatriz Coronato; Cortez, Myrian Bandeira Vianna; Silva, Valmir Laurentino da; Más, Leonora Brazil; Melgarejo, Aníbal Rafael; Bastos, Otilio Machado Pereira

    2014-01-01

    Fecal samples from 56 snakes at the Vital Brazil Institute, in the city of Niterói, Rio de Janeiro, were tested using the sedimentation and flotation techniques to investigate the evolutionary forms of parasites such as helminths and protozoa, and using enzyme immunoassay techniques to detect antigens of Cryptosporidium sp. and Giardia sp. Among the animals tested, 80.3% were positive for parasites. Out of these, there were 16 Bothrops jararaca, 16 B. jararacussu and 13 Crotalus durissus. The prevalence of parasitic nematodes was 41.1%, and nematodes were found in all three snake species. Among these, the most frequent finding was eggs of Kalicephalus sp., which were diagnosed in 25% of the snakes. The positivity for protozoa detected using parasite concentration techniques was 75%, including oocysts of Caryospora sp. in 75%, cysts with morphology similar to Giardia sp. 3.6%, amoeboid cysts in 41.1% and unsporulated coccidia oocysts in 8.9%. Immunoassays for Cryptosporidium sp. antigens produced positive findings in 60.7%. Pseudoparasites were detected in 64.3%. These results show that there is a need to improve the sanitary handling of captive-bred snakes, and also for the animal house that supplies rodents to feed them. The results also highlight that diagnostic tests should be performed periodically on stool specimens from captive-bred snakes.

  5. Ethnobotanic study of Randia aculeata (Rubiaceae in Jamapa, Veracruz, Mexico, and its anti-snake venom effects on mouse tissue

    Directory of Open Access Journals (Sweden)

    CA Gallardo-Casas

    2012-01-01

    Full Text Available In Mexico, medicinal plants are widely used. The use of Randia aculeata by healers against snakebites has never been scientifically tested in relation to possible effects on blood parameters and muscle tissue damage. Interviews were carried out in Jamapa, Veracuz, Mexico, with local residents to collect information about the traditional use of Randia aculeata. In this locality, seven pieces of fruit from the plant are mixed in a liter of alcohol, and then administered orally against snakebites. By using histological techniques and a murine model, we explored its cytoprotective properties against the effects of Crotalus simus and Bothrops asper venoms. Possible protections provided by the plant against tissue damage to skeletal and cardiac muscles and against the typical loss of red blood cells were analyzed. Randia aculeata caused an increase in microhematocrit and total hemoglobin, parameters that are often decremented in association with the loss of red blood cells, which is a characteristic effect of animal venom. Randia aculeata was also shown to protect against the lowering of platelet levels caused by Bothrops asper venom. Finally, Randia aculeata produced a partial inhibition of necrosis following administration of snake venom in skeletal and myocardial muscles. The present results provide solid evidence for the traditional use of Randia aculeata against snakebites, as demonstrated by protection against muscular tissue damage and the diminution of red blood cells.

  6. Evaluación comparativa de dos métodos para determinar la actividad de fosfolipasa A en venenos de serpientes

    Directory of Open Access Journals (Sweden)

    Fanny Lazo

    2014-06-01

    Full Text Available Se ha evaluado la actividad de fosfolipasa A en seis venenos de serpientes comparando las ventajas de un método espectrofotométrico, previamente adaptado a nuestras condiciones de laboratorio, frente al método macroscópico de retardo en la coagulación de una emulsión lipoproteica. En ambos casos, la actividad de fosfolipasa A estuvo presente en los venenos de Micrurus spixii, Crotalus durissus, Bothrops brazili, Lachesis muta y Bothrops atrox y la actividad decreció en orden. En cambio, la actividad de la enzima en el veneno de Micrurus surinamensis sólo fue detectada por el método espectrofotométrico. Así mismo, los resultados basados en la cantidad de veneno utilizado para medir la actividad, mostraron una mayor sensibilidad con el método espectrofotométrico en comparación con el método macroscópico ya que se requirieron cantidades menores en el orden de 2 a 10 veces.

  7. Disease patterns in the Detroit Zoo: a study of reptilian and amphibian populations from 1973 through 1983.

    Science.gov (United States)

    Kaneene, J B; Taylor, R F; Sikarskie, J G; Meyer, T J; Richter, N A

    1985-12-01

    A retrospective study was conducted to determine disease patterns in reptilian and amphibian populations at the Detroit Zoo from 1973 through 1983. In the reptilian population (mean +/- SD = 285.2 +/- 28), overall annual mortality rates were 1% to 40%. Mortality rates were highest in the fall months (20%) and lowest in the winter months (6%). The most frequently affected reptiles were iguana (Iguana iguana), reticulated python (Python reticulatus), rattlesnakes (Crotalus spp), common boa (Constrictor constrictor), and lizards (various genera of suborder Lacertilia). Of the 1,300 reptilian deaths from 1973 through 1983, 36.6% were caused by microbial agents, 12% by parasites, 11.6% by trauma, and 9.3% by nutritional deficiencies. The main microbial organisms that caused death were Aeromonas spp, Salmonella spp, Pseudomonas spp, Proteus spp, and Edwardsiella spp. The main parasites that caused death were Entamoeba spp and lungworms. Among amphibians, frogs and toads were the most frequently affected, and starvation and trauma were the most frequent causes of death.

  8. Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

    Directory of Open Access Journals (Sweden)

    A Della Torre

    2011-01-01

    Full Text Available Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins. The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9 and without (-S9 metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9 and showed signs of mutagenic activity in TA97a and TA102 (both -S9 strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.

  9. Morphologic and morphometric analysis of Hepatozoon spp. (Apicomplexa, Hepatozoidae of snakes

    Directory of Open Access Journals (Sweden)

    Moço Tatiana Cristina

    2002-01-01

    Full Text Available Hepatozoon species are the most abundant hemoparasites of snakes. Its identification has been based mainly on the morphologic characterization of the gamonts in the peripheral blood of the vertebrate host and also of the cysts found in the internal organs of the vertebrate and invertebrate hosts. Using a computerized image analysis system, we studied five species of Hepatozoon from recently captured snakes in Botucatu, State of São Paulo, Brazil, to evaluate the importance of the morphology and morphometry of the gamonts for the characterization of Hepatozoon species and to analyze the morphologic changes induced in the erythrocytes by the parasite. The studied species were H. terzii of Boa constrictor amarali, Hepatozoon sp. of Crotalus durissusterrificus, H. philodryasi of Philodryas patagoniensis, and H. migonei and H. cyclagrasi of Hydrodynastes gigas. We observed three different groups, one of them including the species H. terzii, H. philodryasi and Hepatozoon sp. of C. durissus terrificus; and the other two consisting of H. migonei and H. cyclagrasi. Degree of alterations in the erythrocytes was variable and it may be useful for characterization of Hepatozoon species.

  10. Black Bear Reactions to Venomous and Non-venomous Snakes in Eastern North America

    Science.gov (United States)

    Rogers, Lynn L; Mansfield, Susan A; Hornby, Kathleen; Hornby, Stewart; Debruyn, Terry D; Mize, Malvin; Clark, Rulon; Burghardt, Gordon M

    2014-01-01

    Bears are often considered ecological equivalents of large primates, but the latter often respond with fear, avoidance, and alarm calls to snakes, both venomous and non-venomous, there is sparse information on how bears respond to snakes. We videotaped or directly observed natural encounters between black bears (Ursus americanus) and snakes. Inside the range of venomous snakes in Arkansas and West Virginia, adolescent and adult black bears reacted fearfully in seven of seven encounters upon becoming aware of venomous and non-venomous snakes; but in northern Michigan and Minnesota where venomous snakes have been absent for millennia, black bears showed little or no fear in four encounters with non-venomous snakes of three species. The possible roles of experience and evolution in bear reactions to snakes and vice versa are discussed. In all areas studied, black bears had difficulty to recognize non-moving snakes by smell or sight. Bears did not react until snakes moved in 11 of 12 encounters with non-moving timber rattlesnakes (Crotalus horridus) and four species of harmless snakes. However, in additional tests in this study, bears were repulsed by garter snakes that had excreted pungent anal exudates, which may help explain the absence of snakes, both venomous and harmless, in bear diets reported to date. PMID:25635152

  11. Endogenous hepadnaviruses, bornaviruses and circoviruses in snakes

    Science.gov (United States)

    Gilbert, C.; Meik, J. M.; Dashevsky, D.; Card, D. C.; Castoe, T. A.; Schaack, S.

    2014-01-01

    We report the discovery of endogenous viral elements (EVEs) from Hepadnaviridae, Bornaviridae and Circoviridae in the speckled rattlesnake, Crotalus mitchellii, the first viperid snake for which a draft whole genome sequence assembly is available. Analysis of the draft assembly reveals genome fragments from the three virus families were inserted into the genome of this snake over the past 50 Myr. Cross-species PCR screening of orthologous loci and computational scanning of the python and king cobra genomes reveals that circoviruses integrated most recently (within the last approx. 10 Myr), whereas bornaviruses and hepadnaviruses integrated at least approximately 13 and approximately 50 Ma, respectively. This is, to our knowledge, the first report of circo-, borna- and hepadnaviruses in snakes and the first characterization of non-retroviral EVEs in non-avian reptiles. Our study provides a window into the historical dynamics of viruses in these host lineages and shows that their evolution involved multiple host-switches between mammals and reptiles. PMID:25080342

  12. Oral microbiota of Brazilian captive snakes

    Directory of Open Access Journals (Sweden)

    MG Fonseca

    2009-01-01

    Full Text Available The present work aimed to determine the oral microbiotic composition of snakes from São José do Rio Preto city, São Paulo State, Brazil. Ten snake species, comprising the families Boidae, Colubridae, Elapidae and Viperidae, were submitted to microbiological examination of their oral cavity, which indicated positivity for all buccal samples. Gram-negative bacilli, gram-negative cocci bacilli, gram-positive bacilli and gram-positive cocci were isolated from the snakes. Among isolated bacterium species, the occurrence of coagulase-negative staphylococci in the buccal cavity of Crotalus durissus (Viperiade, Eunectes murinus (Boidae, Mastigodryas bifossatus (Colubridae and Bacillus subtilis, common to oral cavity of Bothrops alternatus (Viperidae and Phalotris mertensi (Colubridae, was detected. It was observed higher diversity of isolated bacteria from the oral cavity of Micrurus frontalis (Elapidae and Philodryas nattereri (Colubridae, as well as the prevalence of gram-positive baccillus and gram-positive cocci. The composition of the oral microbiota of the studied snakes, with or without inoculating fangs, is diverse and also related to the formation of abscesses at the bite site in the victims of the ophidian accidents, and to pathogenic processes in the snakes that host these microorganisms.

  13. Soluble P-selectin rescues viper venom–induced mortality through anti-inflammatory properties and PSGL-1 pathway-mediated correction of hemostasis

    Science.gov (United States)

    Sun, Der-Shan; Ho, Pei-Hsun; Chang, Hsin-Hou

    2016-01-01

    Venomous snakebites are lethal and occur frequently worldwide each year, and receiving the antivenom antibody is currently the most effective treatment. However, the specific antivenom might be unavailable in remote areas. Snakebites by Viperidae usually lead to hemorrhage and mortality if untreated. In the present study, challenges of rattlesnake (Crotalus atrox) venom markedly increased the circulating soluble P-selectin (sP-sel) level, but not P-selectin (P-sel, Selp−/−) mutants, in wild-type mice. Because sP-sel enhances coagulation through the P-selectin ligand 1 (PSGL-1, Selplg) pathway to produce tissue factor–positive microparticles, we hypothesized that increasing the plasma sP-sel level can be a self-rescue response in hosts against snake venom–mediated suppression of the coagulation system. Confirming our hypothesis, our results indicated that compared with wild-type mice, Selp−/− and Selplg−/− mice were more sensitive to rattlesnake venom. Additionally, administration of recombinant sP-sel could effectively reduce the mortality rate of mice challenged with venoms from three other Viperidae snakes. The antivenom property of sP-sel is associated with improved coagulation activity in vivo. Our data suggest that the elevation of endogenous sP-sel level is a self-protective response against venom-suppressed coagulation. The administration of recombinant sP-sel may be developed as a new strategy to treat Viperidae snakebites. PMID:27779216

  14. The use of ecological niche modeling to infer potential risk areas of snakebite in the Mexican state of Veracruz.

    Directory of Open Access Journals (Sweden)

    Carlos Yañez-Arenas

    Full Text Available Many authors have claimed that snakebite risk is associated with human population density, human activities, and snake behavior. Here we analyzed whether environmental suitability of vipers can be used as an indicator of snakebite risk. We tested several hypotheses to explain snakebite incidence, through the construction of models incorporating both environmental suitability and socioeconomic variables in Veracruz, Mexico.Ecological niche modeling (ENM was used to estimate potential geographic and ecological distributions of nine viper species' in Veracruz. We calculated the distance to the species' niche centroid (DNC; this distance may be associated with a prediction of abundance. We found significant inverse relationships between snakebites and DNCs of common vipers (Crotalus simus and Bothrops asper, explaining respectively 15% and almost 35% of variation in snakebite incidence. Additionally, DNCs for these two vipers, in combination with marginalization of human populations, accounted for 76% of variation in incidence.Our results suggest that niche modeling and niche-centroid distance approaches can be used to mapping distributions of environmental suitability for venomous snakes; combining this ecological information with socioeconomic factors may help with inferring potential risk areas for snakebites, since hospital data are often biased (especially when incidences are low.

  15. Parasitological and immunological diagnoses from feces of captive-bred snakes at Vital Brazil Institute

    Directory of Open Access Journals (Sweden)

    Janaína Lima de Souza

    Full Text Available Fecal samples from 56 snakes at the Vital Brazil Institute, in the city of Niterói, Rio de Janeiro, were tested using the sedimentation and flotation techniques to investigate the evolutionary forms of parasites such as helminths and protozoa, and using enzyme immunoassay techniques to detect antigens of Cryptosporidium sp. and Giardiasp. Among the animals tested, 80.3% were positive for parasites. Out of these, there were 16 Bothrops jararaca, 16 B. jararacussu and 13 Crotalus durissus. The prevalence of parasitic nematodes was 41.1%, and nematodes were found in all three snake species. Among these, the most frequent finding was eggs of Kalicephalus sp., which were diagnosed in 25% of the snakes. The positivity for protozoa detected using parasite concentration techniques was 75%, including oocysts of Caryospora sp. in 75%, cysts with morphology similar to Giardia sp. 3.6%, amoeboid cysts in 41.1% and unsporulated coccidia oocysts in 8.9%. Immunoassays for Cryptosporidium sp. antigens produced positive findings in 60.7%. Pseudoparasites were detected in 64.3%. These results show that there is a need to improve the sanitary handling of captive-bred snakes, and also for the animal house that supplies rodents to feed them. The results also highlight that diagnostic tests should be performed periodically on stool specimens from captive-bred snakes.

  16. Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin

    Directory of Open Access Journals (Sweden)

    G. Rádis-Baptista

    2005-12-01

    Full Text Available Snake venom (sv C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD. A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX, from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa and beta (CVXbeta , 12.6 kDa, joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria.

  17. Structural aspects of crotoxin modified by ionizing radiation; Aspectos estruturais da crotoxina modificada pela radiacao ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto de; Albero, Felipe Guimaraes; Zezell, Denise Maria; Spencer, Patrick Jack; Nascimento, Nanci do, E-mail: kcorleto@usp.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2010-03-15

    Ionizing radiation has proven to be an excellent tool for reducing the toxicity of venoms and isolated toxins, resulting in better immunogens for serum production, the only effective treatment in case of snake bites, and contributing to the welfare of serum-producing animals. Since the action of gamma radiation of venoms and toxins has not been yet fully clarified from the structural point of view, we proposed in this paper, to characterize the crotoxin, a venom protein of the species Crotalus durissus terrificus by Circular Dichroism (CD) and Infrared Spectroscopy (FTIR) techniques. After chromatographic techniques, crotoxin was irradiated with 2.0 kGy ({sup 60}Co source). The CD spectra obtained of native and irradiated crotoxin solutions showed changes between the samples in characteristic regions of -sheet and-helix . The Infrared analyse showed expressive changes in the spectra of the native and irradiated crotoxin (amide I band region). These tests showed that crotoxin when subjected to gamma radiation, showed changes in their structural conformation compared with the samples in the native state. Such changes probably occur in the secondary structure and may explain its neurotoxic activity loss. (author)

  18. Specificity analysis of the C-type lectin from rattlesnake venom, and its selectivity towards Gal- or GalNAc-terminated glycoproteins.

    Science.gov (United States)

    Young, N Martin; van Faassen, Henk; Watson, David C; Mackenzie, C Roger

    2011-08-01

    The rattlesnake (Crotalus atrox) venom lectin is a readily-prepared decameric C-type lectin, specific for Gal and GalNAc. Glycan microarray analysis showed it reacted with a wide range of glycans, chiefly recognizing sets of compounds with Galβ1-4GlcNAc (LacNAc), α-Gal or α-GalNAc non-reducing termini. Its array profile was therefore distinctly different from those of four previously studied mammalian C-type lectins with the same Gal/GalNAc monosaccharide specificity, and it was more broadly reactive than several Gal- or GalNAc-specific plant lectins commonly used for glycan blotting. Though a general reactivity towards glycoproteins might be expected from the avidity conferred by its high valence, it showed a marked preference for glycoproteins with multiple glycans, terminated by Gal or GalNAc. Thus its ten closely-spaced sites each with a K(D) for GalNAc of ~2 mM appeared to make RSVL more selective than the four more widely-spaced sites of soybean agglutinin, with a ten-fold better K(D) for GalNAc.

  19. Snakebite cases in the municipalities of the State of Paraiba, Brazil

    Directory of Open Access Journals (Sweden)

    Hellyson Fidel Araujo De Oliveira

    2013-10-01

    Full Text Available Introduction This study investigated the epidemiological and clinical profile of snakebite cases reported from 2007 to 2012 in the municipalities of the Curimataú region, State of Paraíba, in northeastern Brazil. Methods Data were collected from the Health Department of the State of Paraíba using the Injury Notification Information System data banks of the Health Ministry. Results A total of 304 snakebite cases were studied. The cases occurred most frequently from April to June. The genera Bothrops, Crotalus, and Micrurus were responsible for 74.6%, 6.2%, and 1.3% of cases, respectively. Snakebite cases predominated in males living in rural areas and between 10 and 19 years old. The highest incidence of bites occurred on the feet. The majority of the victims received medical assistance within 1 to 3h after being bitten. With regard to severity, 48% of the cases were classified as mild, 26% as moderate, and 2.6% as severe. Successful cures predominated, and no deaths were reported. The average antivenom ampoule dose used for the treatment in some snakebite cases was lower than that recommended by the Health Ministry. Conclusions Although our results show that Paraíba has a good level of medical care, there are serious deficiencies in recording snakebite information. These data indicate the need to improve the recording process for snakebite cases. Further training for health professionals seems to be necessary to optimize their skills in treating snakebite victims.

  20. Skin lipid structure controls water permeability in snake molts.

    Science.gov (United States)

    Torri, Cristian; Mangoni, Alfonso; Teta, Roberta; Fattorusso, Ernesto; Alibardi, Lorenzo; Fermani, Simona; Bonacini, Irene; Gazzano, Massimo; Burghammer, Manfred; Fabbri, Daniele; Falini, Giuseppe

    2014-01-01

    The role of lipids in controlling water exchange is fundamentally a matter of molecular organization. In the present study we have observed that in snake molt the water permeability drastically varies among species living in different climates and habitats. The analysis of molts from four snake species: tiger snake, Notechis scutatus, gabon viper, Bitis gabonica, rattle snake, Crotalus atrox, and grass snake, Natrix natrix, revealed correlations between the molecular composition and the structural organization of the lipid-rich mesos layer with control in water exchange as a function of temperature. It was discovered, merging data from micro-diffraction and micro-spectroscopy with those from thermal, NMR and chromatographic analyses, that this control is generated from a sophisticated structural organization that changes size and phase distribution of crystalline domains of specific lipid molecules as a function of temperature. Thus, the results of this research on four snake species suggest that in snake skins different structured lipid layers have evolved and adapted to different climates. Moreover, these lipid structures can protect, "safety", the snakes from water lost even at temperatures higher than those of their usual habitat.

  1. Protease activated receptors (PARS) mediation in gyroxin biological activity; Mediacao dos receptores ativados por proteases (PARs) em atividades biologicas da giroxina

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Jose Alberto Alves da

    2009-07-01

    Gyroxin is a serine protease enzyme from the South American rattlesnake (Crotalus durissus terrificus) venom; it is only partially characterized and has multiple activities. Gyroxin induces blood coagulation, blood pressure decrease and a neurotoxic behavior named barrel rotation. The mechanisms involved in this neurotoxic activity are not known. Whereas gyroxin is a member of enzymes with high potential to become a new drug with clinical applications such as thrombin, batroxobin, ancrod, tripsyn and kalicrein, it is important to find out how gyroxin works. The analysis on agarose gel electrophoresis and circular dichroism confirmed the molecules' integrity and purity. The gyroxin intravenous administration in mice proved its neurotoxicity (barrel rotation). In vivo studies employing intravital microscopy proved that gyroxin induces vasodilation with the participation of protease activated receptors (PARs), nitric oxide and Na+K+ATPase. The leukocytes' adherence and rolling counting indicated that gyroxin has no pro inflammatory activity. Gyroxin induced platelet aggregation, which was blocked by inhibitors of PAR1 and PAR4 receptors (SCH 79797 and tcY-NH{sub 2}, respectively). Finally, it was proved that the gyroxin temporarily alter the permeability of the blood brain barrier (BBB). Our study has shown that both the protease-activated receptors and nitric oxide are mediators involved in the biological activities of gyroxin. (author)

  2. Genetic characterization of a reptilian calicivirus (Cro1

    Directory of Open Access Journals (Sweden)

    Sandoval-Jaime Carlos

    2012-11-01

    Full Text Available Abstract Background Vesiviruses in the family Caliciviridae infect a broad range of animal hosts including mammals, birds, fish, amphibians and reptiles. The vesivirus Cro1 strains were isolated from diseased snakes in the San Diego zoo in 1978 and reported as the first caliciviruses found in reptiles. The goal of this study was to characterize the Cro1 strain 780032I that was isolated in cell culture from a rock rattlesnake (Crotalus lepidus in the original outbreak. Results We re-amplified the original virus stock in Vero cells, and determined its full-length genome sequence. The Cro1 genome is 8296 nucleotides (nt in length and has a typical vesivirus organization, with three open reading frames (ORF, ORF1 (5643 nt, ORF2 (2121 nt, and ORF3 (348 nt encoding a nonstructural polyprotein, the major capsid protein precursor, and a minor structural protein, respectively. Phylogenetic analysis of the full-length genome sequence revealed that the Cro1 virus clustered most closely with the VESV species of the genus Vesivirus, but was genetically distinct (82-83% identities with closest strains. Conclusions This is the first description of a full-length genome sequence from a reptile calicivirus (Cro1. The availability of the Cro1 genome sequence should facilitate investigation of the molecular mechanisms involved in Cro1 virus evolution and host range.

  3. Walker 256 Tumor Growth Suppression by Crotoxin Involves Formyl Peptide Receptors and Lipoxin A4

    Science.gov (United States)

    Brigatte, Patrícia; Faiad, Odair Jorge; Ferreira Nocelli, Roberta Cornélio; Landgraf, Richardt G.; Palma, Mario Sergio; Cury, Yara; Curi, Rui; Sampaio, Sandra Coccuzzo

    2016-01-01

    We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A4. Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A4 and its natural analogue 15-epi-LXA4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A4 and 15-epi-LXA4, which might inhibit both tumor growth and formation of new vessels via FPRs. PMID:27190493

  4. Sidewinding as a control template for climbing on sand

    Science.gov (United States)

    Marvi, Hamidreza; Gong, Chaohui; Travers, Matthew; Gravish, Nick; Mendelson, Joseph; Hatton, Ross; Choset, Howie; Hu, David; Goldman, Daniel

    2014-03-01

    Sidewinding, translation of a limbless system through lifting of body segments while others remain in static contact with the ground, is used by desert-dwelling snakes like sidewinder rattlesnakes Crotalus cerastes to locomote effectively on hard ground, rocky terrain, and loose sand. Biologically inspired snake robots using a sidewinding gait perform well on hard ground but suffer significant slip when trying to ascend granular inclines. To understand the biological organisms and give robots new capabilities, we perform the first study of mechanics of sidewinding on granular media. We vary the incline angle (0 < θ <20°) of a trackway composed of desert sand. Surface plate drag measurements reveal that as incline angle increases, downhill yield stresses decrease by 50%. Our biological measurements reveal that the animals double the length of the contact region as θ increases; we hypothesize that snakes control this contact to reduce ground shear stress and so avoid slipping. Implementing this anti-slip strategy in a snake robot using contact patch modulation enables the robot to successfully ascend granular inclines.

  5. Biodistribution and SPECT imaging of {sup 125/131}I-crotoxin on mice bearing Ehrlich solid tumour

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Marcella Araugio; Santos, Raquel Gouvea dos [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)], e-mail: marcellaaraugio@yahoo.com.br, e-mail: santosr@cdtn.br; Silveira, Marina B.; Simal, Carlos [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Faculdade de Medicina; Dias, Consuelo L. Fortes [Fundacao Ezequiel Dias (FUNED), Belo Horizonte, MG (Brazil)

    2009-07-01

    The search of specific radiopharmaceuticals to be used in breast tumour diagnosis is relevant to complement the techniques applied in conventional medicine. Crotalus durissus terrificus venom (CV) and its main polypeptide, Crotoxin (Crtx), are natural source of several bioactive substances with therapeutical potential. The aim of this work was to evaluate the binding of Crtx with tumour targets in vivo, as well as, evaluate its applicability for breast tumours diagnosis. Crtx was labelled with {sup 125/131}I using lactoperoxidase method and radiochemical analysis was performed by chromatography. {sup 125}I-Crtx was used for biodistribution and pharmacokinetics studies on swiss mice bearing Ehrlich solid tumour, while {sup 131}I-Crtx was used for single photon emission computed tomography (SPECT) imaging. Crtx presented specific binding sites on Ehrlich tumour cells and had a rapid blood clearance (T{sub 1/2}= 201.1 min.). Intratumoral administration increased significantly the activity delivered into the tumour site (128-fold higher) and reduced the kidney burden (7.2-fold lower). {sup 131}I-Crxt demonstrated to interact with tumour cells for until 72 hours allowing good quality images of tumour. Our results indicate the biotechnological potential of Crtx as template for radiopharmaceutical design for cancer diagnosis. (author)

  6. [Pharmacologic and enzymatic effects of snake venoms from Antioquia and Choco (Colombia)].

    Science.gov (United States)

    Otero, R; Guillermo Osorio, R; Valderrama, R; Augusto Giraldo, C

    1992-01-01

    We compared several pharmacological and enzymatic effects induced by 11 snake venoms from seven species, six of them from different geographic areas of Antioquia and Choco, north-west of Colombia, South America (Bothrops atrox, B. nasutus, B. schlegelii, B. punctatus, Lachesis muta, Micrurus mipartitus), and Crotalus durissus terrificus venom, from specimens captured in other provinces of the country (Tolima, Huila, Meta and Atlantico). Differences were observed in edema-forming, hemorrhage, defibrination, indirect hemolysis, myonecrosis, proteolysis and lethal activity between venoms from different genera or species, as well as according to the geographic area of origin in B. atrox and B. nasutus snake venoms. Bothrops venoms, in particular B. atrox and L. muta, produced major local effects. All of the venoms, including M. mipartitus, had myotoxic effects. The most defibrinating venoms were B. atrox, L. muta, B. punctatus and C. d. terrificus. All of the venoms had indirect hemolytic activity; the venom of M. mipartitus being greatest. The most lethal venoms were those of C. d. terrificus and M. mipartitus. Within Bothrops species, the venom of B. schlegelii was the least active in terms of local and systemic pathologic effects.

  7. Proposta de um Mapa Estratégico para uma Universidade Pública

    Directory of Open Access Journals (Sweden)

    Vinicius Abilio Martins

    2015-08-01

    Full Text Available A ferramenta de tradução da estratégia, medição e avaliação do desempenho organizacional chamado Balanced Scorecard (BSC vem sendo estudada de forma crescente desde a sua publicação em 1990. O formato inicialmente proposto é aplicado muito bem às empresas privadas. Porém, sua aplicação em organizações não pertencentes ao ramo privado não foi contemplado nos estudos iniciais. Este artigo tem por objetivo apresentar uma proposição de um mapa estratégico na forma do Balanced Scorecard para a Universidade Estadual do Oeste do Paraná (UNIOESTE, localizada na região Oeste daquele estado. Foram utilizados dados secundários relacionados ao Plano de Desenvolvimento Institucional, dentre outros documentos relacionados com a estratégia da instituição. Este estudo de caso se classifica como exploratório e qualitativo. A UNIOESTE possui, além da Reitoria, 5 campis localizados nas cidades de Cascavel, Toledo, Foz do Iguaçu, Marechal Cândido Rondon e Francisco Beltrão, além do Hospital Universitário do Oeste do Paraná (HUOP. Conta com corpo docente composto por 1.229 docentes e 1.267 agentes universitários. Para o problema desta pesquisa, tem-se: é possível a criação de um Balanced Scorecard para uma Universidade Pública? Para o tratamento dos dados, utilizou-se a análise de conteúdo, apoiado pelo software ATLAS.ti. Ao final do trabalho, propôs-se um total de 5 perspectivas: Educacional, Financeira, Sociedade, Aprendizado e Crescimento e Processos Internos, distribuídos nestas perspectivas, 16 objetivos estratégicos e 30 indicadores estratégicos. A contribuição deste artigo consiste em apresentar as especificidades não abordadas no modelo tradicional, proposto por Kaplan e Norton, a uma instituição que se apresenta nas características de instituição pública e voltada para o ensino superior.

  8. Tendências de consumo e preço de comercialização do pinhão (semente da Araucaria angustifolia (Bert. O. Ktze., no estado do Paraná / Trends of consumption and commercialization price of the Brazilian-pine nut – seed of Araucaria angustifolia (Bert. O. Ktze., in the state of Parana

    Directory of Open Access Journals (Sweden)

    Rafaelo Balbinot

    2008-09-01

    Full Text Available Este trabalho teve por objetivo estudar a evolução do preço e do volume de comercialização de pinhão, semente da Araucaria angustifolia (Bert. O. Ktze (Pinheiro-do-Paraná, no Estado do Paraná, no período de agosto1994 a fevereiro de 2002. Apesar do importante papel desempenhado poreste produto na economia, principalmente de famílias de baixa renda, e na cultura do Paraná, são quase inexistentes as informações e estudos sobre suas infl uências econômicas. Com a análise dos dados obtidos nas Centrais Estaduais de Abastecimento (CEASA´s de Curitiba, Maringá, Londrina, Foz do Iguaçu e Cascavel, os principais centros consumidoresdo Estado, foi possível observar uma grande variação dos preços, efeito da sazonalidade de sua produção. Também observou-se uma redução no preço real médio da saca de 20 kg do produto de R$25,00 em agosto de 1994 para R$8,47 em julho de 2002 , que corresponde a um preço nominal de R$18,88, sendo que, para manter o valor real de 1994, este teria que estar sendo comercializado a R$53,50. Quanto ao volume, aproximadamente 8,4 mil toneladas (cerca 80,7% do total, foramcomercializadas pelo CEASA de Curitiba, maior centro consumidor do Estado. A variação anual do volume comercializado chegou a 50% entre 2000 e 2001, sendo que a média anual de comercialização é de 1.300 toneladas, com tendência de manter esta média e a variação. Não foi detectada a presença de componente cíclico. Também não foi observada relação entre o volume comercializado e os preços praticados.

  9. Hepatitis B virus genotypes from European origin explains the high endemicity found in some areas from southern Brazil.

    Science.gov (United States)

    Bertolini, Dennis Armando; Gomes-Gouvêa, Michele Soares; Guedes de Carvalho-Mello, Isabel Maria Vicente; Carvalho-Mello, Isabel Maria Vicente Guedes de; Saraceni, Cláudia Patara; Sitnik, Roberta; Grazziotin, Felipe Gobbi; Laurino, Jomar Pereira; Laurindo, Jomar Pereira; Fagundes, Nelson Jurandi Rosa; Carrilho, Flair José; Pinho, João Renato Rebello

    2012-08-01

    Southern Brazil is considered an area of low Hepatitis B endemicity, but some areas of higher endemicity have been described in the Southwest of Paraná and Santa Catarina states. The aim of this study was to evaluate viral genotypes circulating throughout Paraná state. PCR amplification and partial sequencing of the S gene was carried out in 228 samples from HBsAg positive candidate blood donors. Samples have been collected in seven different counties (Cascavel, Curitiba, Foz do Iguaçu, Francisco Beltrão, Maringá, Londrina and Paranaguá). The most common HBV genotype in Paraná state was D (82.9%; 189/228), followed by A (14.1%; 32/228). Genotypes F (1.3%; 3/228), C (1.3%; 3/228) and H (0.4%; 1/228) were also found. Distribution of genotypes was different in the studied counties, but genotype D was the most frequent in all of them. In Francisco Beltrão, all studied samples belonged to genotype D. The high prevalence of HBV genotype D in South of Brazil is explained by the intense migration of settlers from Europeans countries. Subgenotypes A1 and A2 were identified circulating in all cities where HBV/A was found. As observed in other areas of Brazil, HBV/A1 is more frequent than the HBV/A2 in Paraná state and its presence was significantly larger in black and mulatto individuals. Genotype C was found only in individuals with Asian ancestry from Londrina and Maringá. Most HBV/F sequences identified in this study were classified as subgenotype F2a that was previously described in Brazil. The sole case of subgenotype F4 was from Foz do Iguaçu city, near to Northern Argentina, where F4 is highly prevalent. The single genotype H sample was from Curitiba. This is the first case of this genotype described in Brazil. Further studies should be carried out to determine if more genotype H samples can be found in other populations from Brazil.

  10. Geoprocessing applied to the phytosociological survey in permanent plots El geo-procesamiento aplicado al levantamiento fito-sociológico en parcelas permanentes Geoprocessamento aplicado ao levantamento fitossociológico em parcelas permanentes

    Directory of Open Access Journals (Sweden)

    Luiz Gilberto Bertotti

    2009-12-01

    Full Text Available The goal with this work was to create a georeferenced data bank to follow the phytosociological changes of types of trees along the time and its geographic distribution in the field of study. To the phytosociological survey permanent parcels were plotted in the field of study along the floodplain of the rivers Carro Quebrado and Cascavel, both located in the Campus of the Universidade Estadual do Centro Oeste (UNICENTRO – CEDETEG, in Guarapuava city, Parana state. In each part the attributes analyzed were: sociologic position (SP; class of crown (CC; characteristics of trees (CT; quality conditions of the trunk (QCT; conditions (Cs. These parts were put into spaces with use of the Global Positioning System (GPS and the data were sent to the SPRING software with the intention to build a cadastral data bank to follow and analyze the development of the local vegetation. The creation of this data bank allowed following the development of trees in a time scale, showing the evolution of the part of the forest studied.Este trabajo en su objeto pretende crear un banco de informaciones geo-referenciado para acompañar los cambios fitosociológicos de los indivíduos arbóreos a lo largo del tiempo y su distribuccíon geográfica en el área del estudio. Demarcamos parcelas permanentes a lo largo de la planicie de inundación de los rios “Carro  Quebrado” y Cascavel” que están localizados en el campus de la Universidad Estadual Del Centro Oeste del Estado del Paraná, municipalidad de Guarapuava ( UNICENTRO –CEDETEG.En cada parcela de tierra separada evaluamos distintos atributos: PS: localización sociológica; CC: tipo de copa; CA: características de los árboles; CCT: condiciones de calidad del tronco; Cs: otras condiciones.  Estas parcelas fueron especializadas a partir de la utilización del Sistema de  Pocsicionamiento Global (GPS y todos los datos fuero exportados para el sftware SPRING a fin de construir el banco de informaciones

  11. Longitudinal distribution and seasonality of macroalgae in a subtropical stream impacted by organic pollution Distribuição longitudinal e sazonalidade de macroalgas em um riacho subtropical impactado por poluição orgânica

    Directory of Open Access Journals (Sweden)

    Cleto Kaveski Peres

    2010-06-01

    Full Text Available AIM: This study was carried out to assess longitudinal and temporal distribution and the effects of organic pollution on macroalgal communities in a subtropical stream; METHODS: The occurrence and percent cover of stream macroalgae were investigated during seasonal period in four sampling sites along the course of the Cascavel Stream, in Paraná State, subtropical area of Brazil. Sampling sites were randomly chosen; however, their location in relation to urban organic pollution sources was taken into consideration. Besides, several stream variables usually related to organic pollution in aquatic ecosystems were measured; RESULTS: Eleven macroalgal taxa were found during the samplings. Chlorophyta was the prevailing division (4 species or 36.3% of the total richness, followed by Cyanophyta and Rhodophyta (3 or 27.3% and Heterokontophyta (1 or 9.1%. Analysis of variance (ANOVA showed no significant differences in species diversity, percent cover and diversity index among seasons or the sampling sites. In addition, species diversity was not correlated with any environmental variable in particular. On the other hand, the relative composition of the stream macroalgal communities showed wide spatial and temporal variation. Thus, Chlorophyta had higher values of richness and percent cover in sampling site with higher levels of organic pollution, while Rhodophyta was never found in this segment. Taking seasonality into consideration, Chlorophyta had higher values of percent cover in winter, but Rhodophyta and Cyanophyta in spring and fall, respectively; CONCLUSIONS: In general terms, in this study macroalgal community structures were slightly affected by organic pollution, mainly by increase in richness and percent cover of Chlorophyta associated with the absence of Rhodophyta in the most polluted sampling site. The analysis of the richness throughout the stretch of the stream showed that the highest values were found in the intermediate part, being

  12. Gênero e distribuição espacial da população no oeste do Paraná

    Directory of Open Access Journals (Sweden)

    Lucir Reinaldo Alves

    2013-04-01

    Full Text Available Neste artigo analisa-se o perfil da localização e distribuição espacial da população masculina e feminina no oeste do Paraná. Os dados de população coletados pelo Instituto Brasileiro de Geografia e Estatística (IBGE foram desagrupados em quatro variáveis: população urbana feminina, população rural feminina, população urbana masculina e população rural masculina, distribuídos nos cinquenta municípios que compõem a Região. O período estudado abrangeu os anos de 2000 e 2010. Os dados foram analisados a partir de alguns indicadores de localização consagrados pela análise regional. Os resultados mostraram que Cascavel, Foz do Iguaçu e Toledo possuem uma população urbana masculina e feminina mais numerosa que os demais municípios. Já os demais municípios estão consolidando suas economias, seja na transformação agroalimentar, seja na prestação de serviços, fato que se reflete em uma maior participação da população urbana, que cresce mais rapidamente em relação a população rural.Palavras-chave | Distribuição espacial da população; gênero; oeste do Paraná; região.Código JEL | J16; R12; 018. GENDER AND SPATIAL DISTRIBUTION OF POPULATION IN WESTERN PARANÁAbstractThis paper analyzes the profile of the location and spatial distribution of male and female population inWestern Paraná. Population data collected by the Brazilian Institute of Geography and Statistics (IBGE were grouped into four variables: urban female population, rural female population, urban male population and rural male population, distributed in the fifty municipalities that comprise the region. The periods studied are the years of 2000 and 2010. Data were analyzed from some location indicators established by the regional analysis. The results showed that Cascavel, Foz do Iguaçu andToledohave a male and female urban population larger than the other municipalities. The other municipalities are consolidating their economies, whether

  13. Ecological Monitoring and Compliance Program 2014 Report

    Energy Technology Data Exchange (ETDEWEB)

    Hall, Derek B. [National Security Technologies, LLC, Las Vegas, Nevada (United States); Anderson, David C. [National Security Technologies, LLC, Las Vegas, Nevada (United States); Greger, Paul D. [National Security Technologies, LLC, Las Vegas, Nevada (United States); Ostler, W. Kent [National Security Technologies, LLC, Las Vegas, Nevada (United States)

    2015-05-12

    The Ecological Monitoring and Compliance Program (EMAC), funded through the U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO, formerly Nevada Site Office), monitors the ecosystem of the Nevada National Security Site (NNSS) and ensures compliance with laws and regulations pertaining to NNSS biota. This report summarizes the program’s activities conducted by National Security Technologies, LLC (NSTec), during calendar year 2014. Program activities included (a) biological surveys at proposed activity sites, (b) desert tortoise compliance, (c) ecosystem monitoring, (d) sensitive plant species monitoring, (e) sensitive and protected/regulated animal monitoring, and (f) habitat restoration monitoring. During 2014, all applicable laws, regulations, and permit requirements were met, enabling EMAC to achieve its intended goals and objectives. Sensitive and protected/regulated species of the NNSS include 42 plants, 1 mollusk, 2 reptiles, 236 birds, and 27 mammals. These species are protected, regulated, or considered sensitive according to state or federal regulations and natural resource agencies and organizations. The desert tortoise (Gopherus agassizii) and the western yellow-billed cuckoo (Coccyzus americanus) are the only species on the NNSS protected under the Endangered Species Act, both listed as threatened. However, only one record of the cuckoo has ever been documented on the NNSS, and there is no good habitat for this species on the NNSS. It is considered a rare migrant. Biological surveys for the presence of sensitive and protected/regulated species and important biological resources on which they depend were conducted for 18 projects. A total of 199.18 hectares (ha) was surveyed for these projects. Sensitive and protected/regulated species and important biological resources found during these surveys included a predator burrow, one sidewinder rattlesnake (Crotalus cerastes), two mating speckled rattlesnakes

  14. Immunogenicity of Bothrops atrox (Ophidia: Viperidae venom and its evaluation by immunoenzymatic methods Inmunogenicidad del veneno de Bothrops atrox (Ophidia: Viperidae y su evaluación por métodos inmunoenzimáticos

    Directory of Open Access Journals (Sweden)

    Gustavo A. Sandoval

    2012-02-01

    Full Text Available The immunogenicity of Bothrops atrox, “jergón”, venom was studied using ELISA and Western Blot methods, as well as cross-reactivity patterns against venoms of Bothrops brazili, Lachesis muta and Crotalus durissus. For this purpose, New Zealand white rabbits (2 kg aprox were immunized with four 500 μg doses of B. atrox venom in a period of 90 days. Antibody production was followed using ELISA technique, and title of hiper-immune serum was determined at the end of immunization protocol. Additionally, electrophoretic patterns of venoms were analyzed by SDS-PAGE and venom reactivity against obtained serum by ELISA and Western Blot. Immunization schedule allowed a pronounced antibody production since day 20 of protocol. At the end of process, serum title was 256000, which demonstrated both efficacy and usefulness of the developed procedure. On the other hand, studied venoms showed a heterogenic protein composition according to their electrophoretic patterns, whereas cross-reactivity values of 23,7%, 4,0% and 1,8% were obtained between B. atrox venom and B. brazili, L. muta and C. durissus venoms, respectively, using immunoenzymatic methods. According to our results, this procedure constitutes an initial step for further assays directed to optimization in immunoserum production for envenoming treatment and development of kits for diagnosis and species identification of snakes.Se estudió la inmunogenicidad del veneno de la serpiente Bothrops atrox, “jergón”, utilizando los métodos inmunoenzimáticos de ELISA y Western Blot, así como los patrones de reactividad cruzada empleando los venenos de las serpientes Bothrops brazili, Lachesis muta y Crotalus durissus. Para este fin se inmunizaron conejos albinos Nueva Zelanda (2 kg aprox con cuatro dosis de 500 μg del veneno de B. atrox en un periodo de 90 días. La producción de anticuerpos fue monitoreada mediante la técnica de ELISA, determinándose el título del suero hiperinmune obtenido

  15. 蛇毒类凝血酶基因工程的研究进展%Research Progress on Cloning of Snake Venom Thrombin-like Enzymes

    Institute of Scientific and Technical Information of China (English)

    李家祺; 李谦; 唐松山; 李红枝

    2013-01-01

    蛇毒类凝血酶(Thrombin-like enzyme,TLE)是蛇毒中与血浆凝血酶性质相似的一类丝氨酸蛋白酶,所不同的是在蛇毒类凝血酶结构中已经没有纤维蛋白稳定因子激活组分.由于蛇毒类凝血酶在新药研究中扮演重要角色,比如来自Bothrops jararaca和Bothrops atrox蛇毒的立芷雪、来自东北白眉蝮蛇毒的邦停和来自尖吻蝮蛇毒的苏灵,它们是具有止血作用的新药;来自Gloydius shedaoensis和Gloydius ussuriensis蛇毒的东菱克栓酶、来自白眉蝮蛇和尖吻蝮蛇毒的降纤酶、来自Calloselasma rhodostoma蛇毒的安克洛酶和来自Crotalus adamanteus蛇毒的Crotalase,它们是具有溶栓作用的新药.这些药用蛋白质的来源和产量因有限的蛇毒原料而有很大限制,通过基因克隆可解决资源问题.文章综述了蛇毒类凝血酶的基因结构、糖基化特点和各种重组表达体系,为大量制备供临床和基础研究使用奠定基础.%Snake venom thrombin-like enzymes (snTLE) are one kind of serine proteases similar to thrombin from blood, but the basic difference between them is that snTLE has no XIII-activated domain to lead thrombosis in vivo or in vitro. Because snake venoms play very important role in pharmaceutics,for example Reptilase from Bothrops jararaca or Bothrops atrox snake venom, Bangtin from Agkistrodon halys snake venom,or Siding from Agkistrodon acutus snake venom,are hemostatic drugs. Defibrase from Gloydius shedaoensis ,Cloydius ussuriensis,Agkistrodon halys,or Agkistrodon acutus snake venom,Ancrod from Calloselasma rhodostoma snake venom, or Crotalase from Crotalus adamanteus snake venom, is thrombolytic drug. So the shortage of snake venom source can be overcome by using gene recombination method. The gene structures, protein glycosylation, and all kinds of cloning expression systems for snTLE protein are summarized in the paper,which will provide a basis for large-scale production of some snTLE proteins.

  16. Eficacia experimental de anticuerpos IgY producidos en huevos, contra el veneno de la serpiente peruana Bothrops atrox Experimental efficacy of IgY antibodies produced in eggs against the venom of the Peruvian snake Bothrops atrox

    Directory of Open Access Journals (Sweden)

    Julio C. Mendoza

    2012-03-01

    Full Text Available Objetivos. Desarrollar un protocolo de inmunización para producir inmunoglobulinas IgY de origen aviar contra el veneno de la serpiente peruana Bothrops atrox y evaluar la capacidad neutralizante. Materiales y métodos. Se inmunizaron seis gallinas de postura de la raza hy line brown con 500 μg/dosis de veneno de B. atrox en un periodo de dos meses. Cada semana, los huevos fueron colectados para el aislamiento de inmunoglobulinas IgY a partir de la yema, usando dos pasos consecutivos con αcido caprνlico y sulfato de amonio. La detecciσn de anticuerpos se realizσ por inmunodifusiσn doble mientras que el tνtulo y reactividad cruzada se determinaron por las técnicas de ELISA y Western blot. El cálculo de DL50 y de la DE50 del antiveneno IgY producido se realizó utilizando el método de Probits. Resultados. La masa de anticuerpos aislados fue de 8,5 ± 1,35 mg de IgY/mL de yema. Asimismo, la DE50 del antiveneno aviar fue calculada en 575 μL de antiveneno/mg de veneno. Adicionalmente, los ensayos de reactividad cruzada mostraron que el veneno de B. atrox comparte mas epνtopes comunes con el veneno de B. brazili (47% que con otros veneno del mismo género, en tanto que los venenos de Lachesis muta (19% y Crotalus durissus (12% mostraron una baja reactividad cruzada. Conclusiones. Se ha obtenido IgY purificada contra el veneno de B. atrox con capacidad neutralizante y se ha demostrado su utilidad como herramienta inmunoanalítica para evaluar la reactividad cruzada con venenos de otras especies.Objectives. To develop an immunization protocol in order to produce avian IgY immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity. Materials and methods. Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for IgY isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate

  17. Autophagy is involved in cytotoxic effects of crotoxin in human breast cancer cell line MCF-7 cells

    Institute of Scientific and Technical Information of China (English)

    Ci-hui YAN; Ya-ping YANG; Zheng-hong QIN; Zhen-lun GU; Paul REID; Zhong-qin LIANG

    2007-01-01

    Aim: To investigate the role of crotoxin (CrTX)-induced autophagy in the death of MCF-7 cells, a caspase-3-deficient, human breast cancer cell line. Methods: Cul-tured MCF-7 cells were treated with various doses of CrTX, a phospholipase A2(PLA2) isolated from the venom of the South American rattlesnake, Crotalus durissus terrificus. The cytotoxicity of CrTX in the presence and absence of caspase inhibitors was measured with methyl thiazolyl tetrazolium (MTT) and lactate dehydrogenase (LDH) leakage assays. The activation of autophagy was determined with transmission electron microscope and monodansylcadaverin(MDC) labeling. The upregulation of lysosomal enzymes, the release of cyto-chrome c (cyto-c), and the nuclear translocation of the apoptosis inducing factor(AIF) were examined by immunoblotting and immunofluorescence. Results: CrTX inhibited the viability of MCF-7 cells in a dose- and time-dependent manner. CrTX-activated autophagy was revealed by punctuate MDC labeling, and an increase in the formation of autophagosomes as well as apoptosis, as evidenced by nuclear condensation and fragmentation. The activation of cathepsin B, D, and L, in addition to the release of cytochrome c and the relocation of AIF into nuclei, were observed after CrTX treatment. Autophagy inhibitors 3-methyladenine (3-MA),NH4Cl, and the pan-caspase inhibitor, Z-Val-Ala-Asp-fluoromethylketone (Z-Vad-fmk), attenuated CrTX-induced cell death. Conclusion: An autophagic mecha-nism contributes to the apoptosis of MCF-7 cells induced by CrTX.

  18. Anti-tumor activity of CrTX in human lung adenocarcinoma cell line A549

    Institute of Scientific and Technical Information of China (English)

    Bin YE; Yan XIE; Zheng-hong QIN; Jun-chao WU; Rong HAN; Jing-kang HE

    2011-01-01

    Aim:To assess the cytotoxic effect of crotoxin (CrTX),a potent neurotoxin extracted from the venom of the pit viper Crotalus durissus terrificus,in human lung adenocarcinoma A549 cells and investigated the underlying mechanisms.Methods:A549 cells were treated with gradient concentrations of CrTX,and the cell cycle and apoptosis were analyzed using a flow cytometric assay.The changes of cellular effectors p53,caspase-3 and cleaved caspase-3,total P38MAPK and pP38MAPK were investigated using Western blot assays.A549 xenograft model was used to examine the inhibition of CrTX on tumor growth in vivo.Results:Treatment of A549 cells with CrTX (25-200 μg/mL) for 48 h significantly inhibited the cell growth in a dose-dependent manner (IC50=78 μg/mL).Treatment with CrTX (25 iJg/mL) for 24 h caused G1 arrest and induced cell apoptosis.CrTX (25 μg/mL) significantly increased the expression of wt p53,cleaved caspase-3 and phospho-P38MAPK.Pretreatment with the specific P38MAPK inhibitor SB203580 (5 μmol/L) significantly reduced CrTX-induced apoptosis and cleaved caspase-3 level,but G1 arrest remained unchanged and highly expressed p53 sustained.Intraperitoneal injection of CrTX (10 μg/kg,twice a week for 4 weeks) significantly inhibited A549 tumor xenograft growth,and decreased MVD and VEGF levels.Conclusion:CrTX produced significant anti-tumor effects by inducing cell apoptosis probably due to activation of P38MAPK and caspase-3,and by cell cycle arrest mediated by increased wt p53 expression.In addition,CrTX displayed anti-angiogenic effects in vivo.

  19. Effects of PI and PIII Snake Venom Haemorrhagic Metalloproteinases on the Microvasculature: A Confocal Microscopy Study on the Mouse Cremaster Muscle

    Science.gov (United States)

    Herrera, Cristina; Voisin, Mathieu-Benoit; Escalante, Teresa; Rucavado, Alexandra; Nourshargh, Sussan

    2016-01-01

    The precise mechanisms by which Snake Venom Metalloproteinases (SVMPs) disrupt the microvasculature and cause haemorrhage have not been completely elucidated, and novel in vivo models are needed. In the present study, we compared the effects induced by BaP1, a PI SVMP isolated from Bothrops asper venom, and CsH1, a PIII SVMP from Crotalus simus venom, on cremaster muscle microvasculature by topical application of the toxins on isolated tissue (i.e., ex vivo model), and by intra-scrotal administration of the toxins (i.e., in vivo model). The whole tissue was fixed and immunostained to visualize the three components of blood vessels by confocal microscopy. In the ex vivo model, BaP1 was able to degrade type IV collagen and laminin from the BM of microvessels. Moreover, both SVMPs degraded type IV collagen from the BM in capillaries to a higher extent than in PCV and arterioles. CsH1 had a stronger effect on type IV collagen than BaP1. In the in vivo model, the effect of BaP1 on type IV collagen was widespread to the BM of arterioles and PCV. On the other hand, BaP1 was able to disrupt the endothelial barrier in PCV and to increase vascular permeability. Moreover, this toxin increased the size of gaps between pericytes in PCV and created new gaps between smooth muscle cells in arterioles in ex vivo conditions. These effects were not observed in the case of CsH1. In conclusion, our findings demonstrate that both SVMPs degrade type IV collagen from the BM in capillaries in vivo. Moreover, while the action of CsH1 is more directed to the BM of microvessels, the effects of BaP1 are widespread to other microvascular components. This study provides new insights in the mechanism of haemorrhage and other pathological effects induced by these toxins. PMID:27992592

  20. Microbiological evaluation of different strategies for management of snakes in captivity.

    Science.gov (United States)

    Campagner, M V; Bosco, S M G; Bagagli, E; Cunha, M L R S; Jeronimo, B C; Saad, E; Biscola, N P; Ferreira, R S; Barraviera, B

    2012-01-01

    Keeping snakes in captivity to produce venom for scientific research and production of inputs is now a worldwide practice. Maintaining snakes in captivity involves capture, infrastructure investments, management techniques, and appropriate qualified personnel. Further, the success of the project requires knowledge of habitat, nutrition, and reproduction, and control of opportunistic infections. This study evaluated the management of snakes in three types of captivity (quarantine, intensive, and semiextensive) and diagnosed bacterial and fungal contaminants. A bacteriological profile was obtained by swabbing the oral and cloacal cavities, scales, and venoms of healthy adult snakes from Bothrops jararaca (Bj) and Crotalus durissus terrificus (Cdt). There was predominance of Enterobacteriaceae, especially non-fermenting Gram-negative bacilli excluding Pseudomonas spp and Gram- positive bacteria. Statistically, intensive captivity resulted in the highest number of bacterial isolates, followed by recent capture (quarantine) and by semiextensive captivity. No statistical difference was found between Bj and Cdt bacterial frequency. In vitro bacterial susceptibility testing found the highest resistance against the semisynthetic penicillins (amoxicillin and ampicillin) and highest sensitivity to amicacin and tobramycin aminoglycosides. To evaluate mycological profile of snakes from intensive captivity, samples were obtained from two healthy Bj and one B. moojeni, one B. pauloensis, and one Cdt showing whitish lesions on the scales suggestive of ringworm. Using conventional methods and DNA-based molecular procedures, five samples of Trichosporon asahii were identified. Despite the traditional role of intense captivity in ophidian venom production, semiextensive captivity was more effective in the present study by virtue of presenting superior control of bacterial and fungal transmission, easier management, lowest cost, and decreased rate of mortality; therefore, it should be

  1. Crotoxin in humans: analysis of the effects on extraocular and facial muscles

    Directory of Open Access Journals (Sweden)

    Geraldo de Barros Ribeiro

    2012-12-01

    Full Text Available PURPOSE: Crotoxin is the main neurotoxin of South American rattlesnake Crotalus durissus terrificus. The neurotoxic action is characterized by a presynaptic blockade. The purpose of this research is to assess the ability of crotoxin to induce temporary paralysis of extraocular and facial muscles in humans. METHODS: Doses of crotoxin used ranged from 2 to 5 units (U, each unit corresponding to one LD50. We first applied 2U of crotoxin in one of the extraocular muscles of 3 amaurotic individuals to be submitted to ocular evisceration. In the second stage, we applied crotoxin in 12 extraocular muscles of 9 patients with strabismic amblyopia. In the last stage, crotoxin was used in the treatment of blepharospasm in another 3 patients. RESULTS: No patient showed any systemic side effect or change in vision or any eye structure problem after the procedure. The only local side effects observed were slight conjunctival hyperemia, which recovered spontaneously. In 2 patients there was no change in ocular deviation after 2U crotoxin application. Limitation of the muscle action was observed in 8 of the 12 applications. The change in ocular deviation after application of 2U of crotoxin (9 injections was in average 15.7 prism diopters (PD. When the dose was 4U (2 applications the change was in average 37.5 PD and a single application of 5U produced a change of 16 PD in ocular deviation. This effect lasted from 1 to 3 months. Two of the 3 patients with blepharospasm had the hemifacial spasm improved with crotoxin, which returned after 2 months. CONCLUSIONS: This study provides data suggesting that crotoxin may be a useful new therapeutic option for the treatment of strabismus and blepharospasm. We expect that with further studies crotoxin could be an option for many other medical areas.

  2. Study of crotoxin on the induction of paralysis in extraocular muscle in animal model

    Directory of Open Access Journals (Sweden)

    Geraldo de Barros Ribeiro

    2012-10-01

    Full Text Available PURPOSE: Crotoxin is the major toxin of the venom of the South American rattlesnake Crotalus durissus terrificus, capable of causing a blockade of the neurotransmitters at the neuromuscular junction. The objective of this study was to appraise the action and effectiveness of the crotoxin induced paralysis of the extraocular muscle and to compare its effects with the botulinum toxin type A (BT-A. METHODS: The crotoxin, with LD50 of 1.5 µg, was injected into the superior rectus muscle in ten New Zealand rabbits. The concentration variance was 0.015 up to 150 µg. Two rabbits received 2 units of botulinum toxin type A for comparative analysis. The evaluation of the paralysis was performed using serial electromyography. After the functional recovery of the muscles, which occurred after two months, six rabbits were sacrificed for anatomopathology study. RESULTS: The animals did not show any evidence of systemic toxicity. Transitory ptosis was observed in almost every animal and remained up to fourteen days. These toxins caused immediate blockade of the electrical potentials. The recovery was gradual in the average of one month with regeneration signs evident on the electromyography. The paralysis effect of the crotoxin on the muscle was proportional to its concentration. The changes with 1.5 µg crotoxin were similar to those produced by the botulinum toxin type A. The histopathology findings were localized to the site of the injection. No signs of muscle fiber's necrosis were seen in any sample. The alterations induced by crotoxin were also proportional to the concentration and similar to botulinum toxin type A in concentration of 1.5 µg. CONCLUSION: Crotoxin was able to induce transitory paralysis of the superior rectus muscle. This effect was characterized by reduction of action potentials and non-specific signs of fibrillation. Crotoxin, in concentration of 1.5 µg was able to induce similar effects as botulinum toxin type A.

  3. Full-Length Venom Protein cDNA Sequences from Venom-Derived mRNA: Exploring Compositional Variation and Adaptive Multigene Evolution.

    Directory of Open Access Journals (Sweden)

    Cassandra M Modahl

    2016-06-01

    Full Text Available Envenomation of humans by snakes is a complex and continuously evolving medical emergency, and treatment is made that much more difficult by the diverse biochemical composition of many venoms. Venomous snakes and their venoms also provide models for the study of molecular evolutionary processes leading to adaptation and genotype-phenotype relationships. To compare venom complexity and protein sequences, venom gland transcriptomes are assembled, which usually requires the sacrifice of snakes for tissue. However, toxin transcripts are also present in venoms, offering the possibility of obtaining cDNA sequences directly from venom. This study provides evidence that unknown full-length venom protein transcripts can be obtained from the venoms of multiple species from all major venomous snake families. These unknown venom protein cDNAs are obtained by the use of primers designed from conserved signal peptide sequences within each venom protein superfamily. This technique was used to assemble a partial venom gland transcriptome for the Middle American Rattlesnake (Crotalus simus tzabcan by amplifying sequences for phospholipases A2, serine proteases, C-lectins, and metalloproteinases from within venom. Phospholipase A2 sequences were also recovered from the venoms of several rattlesnakes and an elapid snake (Pseudechis porphyriacus, and three-finger toxin sequences were recovered from multiple rear-fanged snake species, demonstrating that the three major clades of advanced snakes (Elapidae, Viperidae, Colubridae have stable mRNA present in their venoms. These cDNA sequences from venom were then used to explore potential activities derived from protein sequence similarities and evolutionary histories within these large multigene superfamilies. Venom-derived sequences can also be used to aid in characterizing venoms that lack proteomic profiles and identify sequence characteristics indicating specific envenomation profiles. This approach, requiring only

  4. Management of Tissue Loss After Agkistrodon Snakebite: Appropriate Use of Crotalidae-Fab Antivenin.

    Science.gov (United States)

    Larson, Kenneth W; Schaefer, Keith R; Austin, Cindy; Norton, Rhy; Finley, Phillip J

    2016-01-01

    Although initially created for the treatment of rattlesnake (genus: Crotalus) bites, Crotalidae-Fab antivenin is used to treat many different pit viper envenomations. However, the efficacy of Crotalidae-Fab in preventing tissue loss from copperhead (Agkistrodon contortrix) or cottonmouth (Agkistrodon piscivorus) snakebites remains unclear. Recent reports show that Agkistrodon-related bites rarely require treatment beyond simple observation and pain control. The purpose of this study was to examine the amount of tissue loss in patients who received Crotalidae-Fab compared with those who did not after an Agkistrodon bite. After institutional review board approval, a retrospective study was completed at a Level 1 trauma center. Between 2009 and 2013, a total of 57 snakebites were identified. Of the 57 bites, the snake species was documented in 36 cases including 31 copperheads, 1 cottonmouth, and 4 rattlesnakes. The other 21 bites were from unknown or nonvenomous species. Of the 32 Agkistrodon-related bites, 15 patients received Crotalidae-Fab (average of 3 vials administered) and 17 did not receive Crotalidae-Fab. None of the 32 patients, regardless of treatment option, had tissue loss or required surgical interventions. Only 1 patient received Crotalidae-Fab and debridement of a vesicle associated with the bite. No clinically significant differences were observed between the groups. These findings support previous literature that failed to show added benefit of Crotalidae-Fab treatment for Agkistrodon bites beyond patient comfort and pain control. Evaluation of current protocols for Agkistrodon envenomations is warranted. Snakebite wound education in trauma physicians and nurses may decrease unnecessary use of antivenom medication.

  5. Using multiscale spatial models to assess potential surrogate habitat for an imperiled reptile.

    Directory of Open Access Journals (Sweden)

    Jennifer M Fill

    Full Text Available In evaluating conservation and management options for species, practitioners might consider surrogate habitats at multiple scales when estimating available habitat or modeling species' potential distributions based on suitable habitats, especially when native environments are rare. Species' dependence on surrogates likely increases as optimal habitat is degraded and lost due to anthropogenic landscape change, and thus surrogate habitats may be vital for an imperiled species' survival in highly modified landscapes. We used spatial habitat models to examine a potential surrogate habitat for an imperiled ambush predator (eastern diamondback rattlesnake, Crotalus adamanteus; EDB at two scales. The EDB is an apex predator indigenous to imperiled longleaf pine ecosystems (Pinus palustris of the southeastern United States. Loss of native open-canopy pine savannas and woodlands has been suggested as the principal cause of the species' extensive decline. We examined EDB habitat selection in the Coastal Plain tidewater region to evaluate the role of marsh as a potential surrogate habitat and to further quantify the species' habitat requirements at two scales: home range (HR and within the home range (WHR. We studied EDBs using radiotelemetry and employed an information-theoretic approach and logistic regression to model habitat selection as use vs.We failed to detect a positive association with marsh as a surrogate habitat at the HR scale; rather, EDBs exhibited significantly negative associations with all landscape patches except pine savanna. Within home range selection was characterized by a negative association with forest and a positive association with ground cover, which suggests that EDBs may use surrogate habitats of similar structure, including marsh, within their home ranges. While our HR analysis did not support tidal marsh as a surrogate habitat, marsh may still provide resources for EDBs at smaller scales.

  6. Structural correlates of speed and endurance in skeletal muscle: the rattlesnake tailshaker muscle

    Science.gov (United States)

    Schaeffer; Conley; Lindstedt

    1996-01-01

    The western diamondback rattlesnake Crotalus atrox can rattle its tail continuously for hours at frequencies approaching 90 Hz. We examined the basis of these fast sustainable contractions using electromyography, data on oxygen uptake and the quantitative ultrastructure of the tailshaker muscle complex. The tailshaker muscle has no apparent unique structures; rather, the relative proportions of the structures common to all skeletal muscles appear to be present (1) to minimize activation, contraction and relaxation times via an extremely high volume density of sarcoplasmic reticulum (26 %) as well as, (2) to maximize ATP resysnthesis via a high volume density of mitochondria (26 %). The high rate of ATP supply is reflected in the in vivo muscle mass-specific oxygen uptake of this group of muscles which, at 585 ml O2 kg-1 min-1 during rattling at 30 °C body temperature, exceeds that reported for other ectotherm and many endotherm muscles. Since the change in oxygen uptake paralleled that of the rattling frequency over the range of measured body temperatures, there was a nearly constant O2 cost per muscle contraction (0.139±0.016 µl O2 g-1). Electromyo-graphic analysis suggests that each of the six muscles that make up the shaker complex may be a single motor unit. Finally, the maximum rate of mitochondrial oxygen uptake is similar to that of various mammals, a hummingbird, a lizard, an anuran amphibian and of isolated mitochondria (at 10 000-40 000 molecules O2 s-1 µm2 of cristae surface area, when normalized to 30 °C), suggesting a shared principle of design of the inner mitochondrial membrane among the vertebrates.

  7. Novo método de dosagem de soros antipeçonhentos em camundongos lactentes: I - dosagem do soro anticrotálico

    Directory of Open Access Journals (Sweden)

    Bruno Soerensen

    1984-08-01

    Full Text Available A atividade biológica dos soros antipeçonhentos pode ser determinada "in vivo" em pombos, coelhos, cobaios e camundongos adultos, não existindo, entretanto, nenhum método que possa ser recomendado internacionalmente. Estudou-se comparativamente aos métodos tradicionais de dosagem de soros em pombos e camundongos adultos, a validade do uso de camundongos lactentes de 6 a 7 dias, pesando 4 a 5g, inoculados pela via subcutânea. Inicialmente foi determinada a toxidez do veneno de Crotalus durissus terríficus através do estudo da sintomatologia do envenenamento e da atividade letal. O estudo comparativo dos três métodos forneceu maior concordância de resultados em DE100 entre as dosagens realizadas com camundongos lactentes e adultos. A DE100 e DL50 determinadas em camundongos lactentes forneceu resultados mais constantes que os dos métodos de camundongos adultos e pombos. O uso deste novo método permite eliminar a dificuldade de obtenção do atual animal de prova; a utilização de um maior número de animais por ponto de avaliação biológica, possibilitando uma maior precisão e conseguindo-se uma uniformidade nas características exigidas neste tipo de dosagem como peso, idade e linhagem, visando a reprodução sistemática dos resultados.

  8. Crovirin, a Snake Venom Cysteine-Rich Secretory Protein (CRISP) with Promising Activity against Trypanosomes and Leishmania

    Science.gov (United States)

    Adade, Camila M.; Carvalho, Ana Lúcia O.; Tomaz, Marcelo A.; Costa, Tatiana F. R.; Godinho, Joseane L.; Melo, Paulo A.; Lima, Ana Paula C. A.; Rodrigues, Juliany C. F.; Zingali, Russolina B.; Souto-Padrón, Thaïs

    2014-01-01

    Background The neglected human diseases caused by trypanosomatids are currently treated with toxic therapy with limited efficacy. In search for novel anti-trypanosomatid agents, we showed previously that the Crotalus viridis viridis (Cvv) snake venom was active against infective forms of Trypanosoma cruzi. Here, we describe the purification of crovirin, a cysteine-rich secretory protein (CRISP) from Cvv venom with promising activity against trypanosomes and Leishmania. Methodology/Principal Findings Crude venom extract was loaded onto a reverse phase analytical (C8) column using a high performance liquid chromatographer. A linear gradient of water/acetonitrile with 0.1% trifluoroacetic acid was used. The peak containing the isolated protein (confirmed by SDS-PAGE and mass spectrometry) was collected and its protein content was measured. T. cruzi trypomastigotes and amastigotes, L. amazonensis promastigotes and amastigotes and T. brucei rhodesiense procyclic and bloodstream trypomastigotes were challenged with crovirin, whose toxicity was tested against LLC-MK2 cells, peritoneal macrophages and isolated murine extensor digitorum longus muscle. We purified a single protein from Cvv venom corresponding, according to Nano-LC MS/MS sequencing, to a CRISP of 24,893.64 Da, henceforth referred to as crovirin. Human infective trypanosomatid forms, including intracellular amastigotes, were sensitive to crovirin, with low IC50 or LD50 values (1.10–2.38 µg/ml). A considerably higher concentration (20 µg/ml) of crovirin was required to elicit only limited toxicity on mammalian cells. Conclusions This is the first report of CRISP anti-protozoal activity, and suggests that other members of this family might have potential as drugs or drug leads for the development of novel agents against trypanosomatid-borne neglected diseases. PMID:25330220

  9. Pentastomídeos de répteis do Brasil: revisão dos cephalobaenidae

    Directory of Open Access Journals (Sweden)

    A. Arandas Rêgo

    1983-12-01

    Full Text Available Foram estudados os Cephalobaenidae (Pentastomida, depositados na coleção helmintológica do Instituto Oswaldo Cruz e na coleção de parasitologia do Instituto Butantan. São redescritas e discutidas as espécies, Cephalobaena tetrapoda, C. freitasi, C. giglioli, Raillietiella furcocerca e Mahafaliella venteli. Esses parasitas foram coletados dos répteis: Lachesis sp., Drymarchon c. corais, Xenodon merremii, Crotatus terrificus, Amphisbaena sp., Tropidurus torquatus, Bothrops atrox, Mabuya punctata e de Bufo paracnemis (anfíbio.In this work the author studies Cephalobaenidae parasites using specimens from the helminthological collection of the Oswaldo Cruz and Butantan Institutes. This material was collected from Lachesis sp., Drymarchon corais, Xenodon merremii, Crotalus terrificus, Amphisbaena sp., Tropidurus torquatus, Bothrops atrox, Mabuya punctata (Reptilia and Bufo paracnemis (Amphibia. The species studied are Cephalobaena tetrapoda Heymons, 1922, Cephalobaena giglioli (Hett, 1924 comb. n., Cephalobaena freitasi (Motta & Gomes, 1968 comb. n., Raillietiella furcocerca (Diesing, 1836 and Mahafaliella venteli (Motta, 1965. C. recurvocauda becomes a synonym of C. tetrapoda and as do the specimens that Motta called erronously R. furcocerca. Raillietiella giglioli is changed to Cephalobaena giglioli (Hett, 1924 comb. n. The author describes here the male of C. giglioli for the first time. Travassostulida freitasi and T. acutiacanthus enter in synonymy with C. freitasi, and T. acutiacanthus is considered to be a subspecies of C. freitasi. Raillietiella gomesi becomes a synonym of R. furcocerca. The author discusses Mahafaliella venteli, and also questions the validity of the genus Gretillaria proposed by Motta for some species of Raillietiella. The latter is considered a synonym of Raillietiella.

  10. Ação neuro-muscular do veneno crotálico: dados preliminares Neuromuscular action of crotalid venom: preliminar data

    Directory of Open Access Journals (Sweden)

    Maria Dorvalina Silva

    1996-03-01

    Full Text Available Estudamos 6 pacientes, 2 cães e um coelho com intoxicação crotálica. Avaliamos a condução nervosa periférica sensitiva e motora, a transmissão neuromuscular e eletromiografias. As biópsias de músculo foram processadas por histoquímica. Os 6 pacientes apresentaram mononeuropatia sensitiva no nervo periférico adjacente ao local da inoculação do veneno e encontramos evidências histoquímicas de miopatia mitocondrial. Os defeitos da transmissão neuromuscular foram mínimos. A maioria dos autores admite que veneno crotálico determina síndrome miastênica. Nossos achados indicam que ptose palpebral, facies miastênico e fraqueza muscular observados após acidente crotálico, correspondem provavelmente a miopatia mitocondrial, muitas vezes transitória e reversível.We studied 6 patients and 2 dogs that have been bitten by South American rattlesnake Crotalus durissus terrificus and one rabbit inoculated with crotalid venom. We analized sensory and motor peripheral nerve conduction, repetitive stimulation for studying neuromuscular transmission and electromyographies. Muscle biopsies were processed by histochemistry. All patients had peripheral mononeuropathy of the closest sensitive nerve to the area of snakebite. The neuromuscular transmission alterations were minimal. Muscle histochemistry of 4 patients, 2 dogs and 1 rabbit showed findings of mitochondrial myopathy. The majority of authors admit that crotalid venom causes myastenic syndrome. Our findings suggest that palpebral ptosis, myastenic facies and muscular weakness observed after crotalid poisoning are, probably, due to transient and reversible mitochondrial myopathy. As far as we know, this is the first report on the ability of the venom of this rattlesnake to cause local sensitive mononeuropathy and the first muscle histochemistry showing mitochondrial myopathy in humans poisoned by crotalid venom.

  11. Epidemiologia do acidente por serpentes peçonhentas: estudo de casos atendidos em 1988

    Directory of Open Access Journals (Sweden)

    Lindioneza Adriano Ribeiro

    1995-10-01

    Full Text Available Foram avaliados aspectos epidemiológicos de acidentes por serpentes peçonhentas no Estado de São Paulo, Brasil, com base em prontuários de 322 pacientes e em entrevistas feitas com 209 deles e/ou seus acompanhantes. Os acidentes ocorreram principalmente com pessoas de 10 a 20 anos de idade, do sexo masculino, nos meses de outubro a abril e no período diurno. As regiões anatômicas mais freqüentemente picadas foram os pés, as mãos e as pernas. Bothrops, Crotalus e Micrurus foram responsáveis por, respectivamente, 95,0%, 4,4% e 0,6% dos casos. Não ocorreram óbitos, mas 2,2% dos pacientes apresentaram seqüelas. Dentre os 209 entrevistados, a ocupação de lavrador foi a mais freqüentemente relacionada ao acidente que, em aproximadamente 60% das vezes, ocorreu durante o trabalho. O total de 160 pacientes (76,6% submeteram-se a alguma forma de tratamento antes de chegarem a um serviço de saúde: foram mais comuns o uso de torniquete (50,2%, a expressão local na tentativa de retirar parte do veneno (33,5%, a colocação das mais diversas substâncias sobre o local da picada (36,8% e a ingestão de outras (12,9%; pouco mais de um quarto dos pacientes submeteram-se a alguma forma de tratamento médico antes de chegar ao HVB sendo mais comum a antissepsia (8,2%, a administração do antiveneno (6,2%, de anti-histamínicos (5,7% e de analgésicos (5,3%.

  12. Epidemiologia do acidente por serpentes peçonhentas: estudo de casos atendidos em 1988

    Directory of Open Access Journals (Sweden)

    Ribeiro Lindioneza Adriano

    1995-01-01

    Full Text Available Foram avaliados aspectos epidemiológicos de acidentes por serpentes peçonhentas no Estado de São Paulo, Brasil, com base em prontuários de 322 pacientes e em entrevistas feitas com 209 deles e/ou seus acompanhantes. Os acidentes ocorreram principalmente com pessoas de 10 a 20 anos de idade, do sexo masculino, nos meses de outubro a abril e no período diurno. As regiões anatômicas mais freqüentemente picadas foram os pés, as mãos e as pernas. Bothrops, Crotalus e Micrurus foram responsáveis por, respectivamente, 95,0%, 4,4% e 0,6% dos casos. Não ocorreram óbitos, mas 2,2% dos pacientes apresentaram seqüelas. Dentre os 209 entrevistados, a ocupação de lavrador foi a mais freqüentemente relacionada ao acidente que, em aproximadamente 60% das vezes, ocorreu durante o trabalho. O total de 160 pacientes (76,6% submeteram-se a alguma forma de tratamento antes de chegarem a um serviço de saúde: foram mais comuns o uso de torniquete (50,2%, a expressão local na tentativa de retirar parte do veneno (33,5%, a colocação das mais diversas substâncias sobre o local da picada (36,8% e a ingestão de outras (12,9%; pouco mais de um quarto dos pacientes submeteram-se a alguma forma de tratamento médico antes de chegar ao HVB sendo mais comum a antissepsia (8,2%, a administração do antiveneno (6,2%, de anti-histamínicos (5,7% e de analgésicos (5,3%.

  13. In Vitro Antiophidian Mechanisms of Hypericum brasiliense Choisy Standardized Extract: Quercetin-Dependent Neuroprotection

    Directory of Open Access Journals (Sweden)

    Cháriston André Dal Belo

    2013-01-01

    Full Text Available The neuroprotection induced by Hypericum brasiliense Choisy extract (HBE and its main active polyphenol compound quercetin, against Crotalus durissus terrificus (Cdt venom and crotoxin and crotamine, was enquired at both central and peripheral mammal nervous system. Cdt venom (10 μg/mL or crotoxin (1 μg/mL incubated at mouse phrenic nerve-diaphragm preparation (PND induced an irreversible and complete neuromuscular blockade, respectively. Crotamine (1 μg/mL only induced an increase of muscle strength at PND preparations. At mouse brain slices, Cdt venom (1, 5, and 10 μg/mL decreased cell viability. HBE (100 μg/mL inhibited significantly the facilitatory action of crotamine (1 μg/mL and was partially active against the neuromuscular blockade of crotoxin (1 μg/mL (data not shown. Quercetin (10 μg/mL mimicked the neuromuscular protection of HBE (100 μg/mL, by inhibiting almost completely the neurotoxic effect induced by crotoxin (1 μg/mL and crotamine (1 μg/mL. HBE (100 μg/mL and quercetin (10 μg/mL also increased cell viability in mice brain slices. Quercetin (10 μg/mL was more effective than HBE (100 μg/mL in counteracting the cell lysis induced by Cdt venom (1 and 10 μg/mL, resp.. These results and a further phytochemical and toxicological investigations could open new perspectives towards therapeutic use of Hypericum brasiliense standardized extract and quercetin, especially to counteract the neurotoxic effect induced by snake neurotoxic venoms.

  14. Structural analysis of irradiated crotoxin by spectroscopic techniques

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina C. de; Fucase, Tamara M.; Silva, Ed Carlos S. e; Chagas, Bruno B.; Buchi, Alisson T.; Viala, Vincent L.; Spencer, Patrick J.; Nascimento, Nanci do, E-mail: kcorleto@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Biotecnologia

    2013-07-01

    Snake bites are a serious public health problem, especially in subtropical countries. In Brazil, the serum, the only effective treatment in case of snake bites, is produced in horses which, despite of their large size, have a reduced lifespan due to the high toxicity of the antigen. Ionizing radiation has been successfully employed to attenuate the biological activity of animal toxins. Crotoxin, the main toxic compound from Crotalus durissus terrificus (Cdt), is a heterodimeric protein composed of two subunits: crotapotin and phospholipase A{sub 2}. Previous data indicated that this protein, following irradiation process, undergoes unfolding and/or aggregation, resulting in a much lower toxic antigen. The exact mechanisms and structural modifications involved in aggregation process are not clear yet. This work investigates the effects of ionizing radiation on crotoxin employing Infrared Spectroscopy, Circular Dichroism and Dynamic Light Scattering techniques. The infrared spectrum of lyophilized crotoxin showed peaks corresponding to the vibrational spectra of the secondary structure of crotoxin, including β-sheet, random coil, α-helix and β-turns. We calculated the area of these spectral regions after adjusting for baseline and normalization using the amide I band (1590-1700 cm{sup -1}), obtaining the variation of secondary structures of the toxin following irradiation. The Circular Dichroism spectra of native and irradiated crotoxin suggests a conformational change within the molecule after the irradiation process. This data indicates structural changes between the samples, apparently from ordered conformation towards a random coil. The analyses by light scattering indicated that the irradiated crotoxin formed multimers with an average molecular radius 100 folds higher than the native toxin. (author)

  15. Development of snake-directed antipredator behavior by wild white-faced capuchin monkeys: I. Snake-species discrimination.

    Science.gov (United States)

    Meno, Whitney; Coss, Richard G; Perry, Susan

    2013-03-01

    Young animals are known to direct alarm calls at a wider range of species than adults. Our field study examined age-related differences in the snake-directed antipredator behavior of infant, juvenile, and adult white-faced capuchin monkeys (Cebus capucinus) in terms of alarm calling, looking behavior, and aggressive behavior. In the first experiment, we exposed infant and juvenile white-faced capuchins to realistic-looking inflatable models of their two snake predators, the boa constrictior (Boa constrictor) and neotropical rattlesnake (Crotalus durissus) and a white airplane as a novel control. In the second experiment, infants, juveniles, and adults were presented photographic models of a coiled boa constrictor, rattlesnake, indigo snake (Drymarchon corais), a noncapuchin predator, and a white snake-like model. We found that antipredator behavior changed during the immature stage. Infants as young as 4 months old were able to recognize snakes and display antipredator behavior, but engaged in less snake-model discrimination than juveniles. All age classes exhibited a lower response to the white snake-like model, indicating that the absence of color and snake-scale patterns affected snake recognition. Infants also showed a higher level of vigilance after snake-model detection as exhibited by a higher proportion of time spent looking and head cocking at the models. Aggressive antipredator behavior was found in all age classes, but was more prevalent in juveniles and adults than infants. This study adds to the knowledge of development of antipredator behavior in primates by showing that, although alarm calling behavior and predator recognition appear at a very young age in capuchins, snake-species discrimination does not become apparent until the juvenile stage.

  16. Prevalence of common thrombophilia markers and risk factors in Indian patients with primary venous thrombosis

    Directory of Open Access Journals (Sweden)

    Mahendra Narain Mishra

    Full Text Available CONTEXT AND OBJECTIVE: Venous thrombosis occurs as a result of interaction of genetic and acquired factors including activated protein C resistance (APC-R, fibrinogen levels, antithrombin, protein C, protein S, lupus anticoagulants and anticardiolipin antibodies. This study was aimed at determining the prevalence of these common thrombophilia markers in Asian Indians with primary venous thrombosis. DESIGN AND SETTING: This was a cross-sectional study carried out in Mumbai. METHODS: Samples from 78 patients with a confirmed diagnosis of venous thrombosis and 50 controls were tested. Semi-quantitative estimation (functional assays of protein C, protein S and antithrombin was performed. Quantitative estimation of fibrinogen was done using the Clauss method. Lupus anticoagulants were screened using lupus-sensitive activated partial thromboplastin time and β2-glycoprotein-I dependent anticardiolipin antibodies were estimated by ELISA. APC-R was measured using a clotting-based method with factor V deficient plasma and Crotalus viridis venom. Statistical analysis was performed using Epi-info (version 6. RESULTS: The popliteal vein was the most commonly involved site. Forty-four samples (56% gave abnormal results. The commonest were elevated fibrinogen and APC-R (17.9% each, followed by low protein S (16.6%. CONCLUSIONS: This study confirms the literature findings that fibrinogen level estimation and screening for APC-R are important for the work-up on venous thrombosis patients since these, singly or in combination, may lead to a primary thrombotic episode. The frequency of the other thrombophilia markers was higher among the patients than among the controls, but without statistically significant difference.

  17. A rational design for the nanoencapsulation of poisonous animal venoms in liposomes prepared with natural phospholipids.

    Science.gov (United States)

    da Costa, Maria Helena Bueno; Sant'Anna, Osvaldo A; Quintilio, Wagner; Schwendener, Reto Albert; de Araujo, Pedro Soares

    2012-11-01

    Liposomes have been used since the 1970's to encapsulate drugs envisaging enhancement in efficacy and therapeutic index, avoidance of side effects and increase in the encapsulated agent stability. The major problem when encapsulating snake venoms is the liposomal membrane instability caused by venom phospholipases. Here the results obtained encapsulating Crotalus durissimus terrificus and a pool of Bothropic venoms within liposomes (LC and LB, respectively) used to produce anti-venom sera are presented. The strategy was to modify the immunization protocol to enhance antibody production and to minimize toxic effects by encapsulating inactivated venoms within stabilized liposomes. Chemically modified venoms were solubilized in a buffer containing an inhibitor and a chelating agent. The structures of the venoms were analyzed by UV, CD spectroscopy and ELISA. In spite of the differences in the helical content between natural and modified venoms, they were recognized by horse anti-sera. To maintain long-term stability, mannitol was used as a cryoprotectant. The encapsulation efficiencies were 59 % (LB) and 99 % (LC), as followed by filtration on Sephacryl S1000. Light scattering measurements led us to conclude that both, LB (119 ±47 nm) and LC (147±56 nm) were stable for 22 days at 4 °C, even after lyophilization. Genetically selected mice and mixed breed horses were immunized with these formulations. The animals did not show clinical symptoms of venom toxicity. Both, LB and LC enhanced by at least 30 % the antibody titers 25 days after injection and total IgG titers remained high 91 days after immunization. The liposomal formulation clearly exhibited adjuvant properties.

  18. Rattlesnake Phospholipase A2 Increases CFTR-Chloride Channel Current and Corrects ∆F508CFTR Dysfunction: Impact in Cystic Fibrosis.

    Science.gov (United States)

    Faure, Grazyna; Bakouh, Naziha; Lourdel, Stéphane; Odolczyk, Norbert; Premchandar, Aiswarya; Servel, Nathalie; Hatton, Aurélie; Ostrowski, Maciej K; Xu, Haijin; Saul, Frederick A; Moquereau, Christelle; Bitam, Sara; Pranke, Iwona; Planelles, Gabrielle; Teulon, Jacques; Herrmann, Harald; Roldan, Ariel; Zielenkiewicz, Piotr; Dadlez, Michal; Lukacs, Gergely L; Sermet-Gaudelus, Isabelle; Ollero, Mario; Corringer, Pierre-Jean; Edelman, Aleksander

    2016-07-17

    Deletion of Phe508 in the nucleotide binding domain (∆F508-NBD1) of the cystic fibrosis transmembrane regulator (CFTR; a cyclic AMP-regulated chloride channel) is the most frequent mutation associated with cystic fibrosis. This mutation affects the maturation and gating of CFTR protein. The search for new high-affinity ligands of CFTR acting as dual modulators (correctors/activators) presents a major challenge in the pharmacology of cystic fibrosis. Snake venoms are a rich source of natural multifunctional proteins, potential binders of ion channels. In this study, we identified the CB subunit of crotoxin from Crotalus durissus terrificus as a new ligand and allosteric modulator of CFTR. We showed that CB interacts with NBD1 of both wild type and ∆F508CFTR and increases their chloride channel currents. The potentiating effect of CB on CFTR activity was demonstrated using electrophysiological techniques in Xenopus laevis oocytes, in CFTR-HeLa cells, and ex vivo in mouse colon tissue. The correcting effect of CB was shown by functional rescue of CFTR activity after 24-h ΔF508CFTR treatments with CB. Moreover, the presence of fully glycosylated CFTR was observed. Molecular docking allowed us to propose a model of the complex involving of the ABCβ and F1-like ATP-binding subdomains of ΔF508-NBD1. Hydrogen-deuterium exchange analysis confirmed stabilization in these regions, also showing allosteric stabilization in two other distal regions. Surface plasmon resonance competition studies showed that CB disrupts the ∆F508CFTR-cytokeratin 8 complex, allowing for the escape of ∆F508CFTR from degradation. Therefore CB, as a dual modulator of ΔF508CFTR, constitutes a template for the development of new anti-CF agents.

  19. Venoms, toxins and derivatives from the Brazilian fauna: valuable sources for drug discovery.

    Science.gov (United States)

    De Marco Almeida, Flávia; de Castro Pimenta, Adriano Monteiro; Oliveira, Mônica Cristina; De Lima, Maria Elena

    2015-06-25

    Animal venoms have been widely investigated throughout the world. The great number of biotechnological articles as well as patent applications in the field of drug discovery based on these compounds indicates how important the source is. This review presents a list of the most studied Brazilian venomous animal species and shows the most recent patent applications filed from 2000 to 2013, which comprise Brazilian venoms, toxins and derivatives. We analyze the data according to the species, the type of products claimed and the nationality of the inventors. Fifty-five patent applications were found, involving 8 genera. Crotalus, Lachesis, Bothrops and Loxosceles represented 78% of the patent applications. The other 22% were represented by Phoneutria, Tityus, Acanthoscurria and Phyllomedusa. Most of the inventions (42%) involved anticancer, immunomodulator or antimicrobial drugs, while 13% involved anti-venoms and vaccines, 11% involved hypotensive compositions, 9% involved antinociceptive and/or anti-inflammatory compositions, and the other 25% involved methods, kits or compositions for various purposes. Brazilian inventors filed 49% of the patent applications, but other countries, mainly the United States of America, Germany, Russia and France, also filed patent applications claiming products comprising venoms, toxins and/or derivatives from the Brazilian fauna. Brazil holds an important number of patent applications which mostly belong to universities and research institutes, but the pharmaceutical industry in this field is still weak in Brazil. Although, Brazilian venomous animal species have been reported in drug discovery throughout the world, many species remain to be explored as valuable and promising tools for drug discovery and development.

  20. Biochemical and pharmacological characterization of irradiated crotamine by gamma rays of {sup 60}Co; Caracterizacao bioquimica e farmacologica da crotamina irradiada por raios gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto

    2014-07-01

    The serum production in Brazil, the only effective treatment in cases of snakebites, uses horses that although large size, have reduced l lifespan compared with horses not immunized. Ionizing radiation has been shown as an excellent tool in reducing the toxicity of venoms and toxins isolated, and promote the achievement of better immunogens for serum production, and contributing to the welfare of serum-producing animals. It is known, however, that the effects of ionizing radiation on protein are characterized by various chemical modifications, such as fragmentation, cross-linking due to aggregation and oxidation products generated by water radiolysis. However, the action of gamma radiation on toxins is not yet fully understood structurally and pharmacologically, a fact that prevents the application of this methodology in the serum production process. So we proposed in this paper the characterization of crotamine, an important protein from the venom of Crotalus durissus terrificus species, irradiated with {sup 60}Co gamma rays. After isolating the toxin by chromatographic techniques and testing to prove the obtaining of pure crotamine, it was irradiated with gamma rays and subjected to structural analysis, Fluorescence and Circular Dichroism. Using high hydrostatic pressure tests were also conducted in order to verify that the conformational changes caused by radiation suffer modifications under high pressures. From the pharmacological point of view, muscle contraction tests were conducted with the objective of limiting the action of crotamine in smooth muscle as well as the change in the action of toxin caused structural changes to the front. Analysis of Circular Dichroism and Fluorescence showed changes in structural conformation of crotamine when subjected to gamma radiation and that such changes possibly occurring in the secondary and tertiary structure of the protein. The observed in pharmacological tests showed that the irradiated crotamine was less effective

  1. Vipericidins: a novel family of cathelicidin-related peptides from the venom gland of South American pit vipers.

    Science.gov (United States)

    Falcao, C B; de La Torre, B G; Pérez-Peinado, C; Barron, A E; Andreu, D; Rádis-Baptista, G

    2014-11-01

    Cathelicidins are phylogenetically ancient, pleiotropic host defense peptides-also called antimicrobial peptides (AMPs)-expressed in numerous life forms for innate immunity. Since even the jawless hagfish expresses cathelicidins, these genetically encoded host defense peptides are at least 400 million years old. More recently, cathelicidins with varying antipathogenic activities and cytotoxicities were discovered in the venoms of poisonous snakes; for these creatures, cathelicidins may also serve as weapons against prey and predators, as well as for innate immunity. We report herein the expression of orthologous cathelicidin genes in the venoms of four different South American pit vipers (Bothrops atrox, Bothrops lutzi, Crotalus durissus terrificus, and Lachesis muta rhombeata)-distant relatives of Asian cobras and kraits, previously shown to express cathelicidins-and an elapid, Pseudonaja textilis. We identified six novel, genetically encoded peptides: four from pit vipers, collectively named vipericidins, and two from the elapid. These new venom-derived cathelicidins exhibited potent killing activity against a number of bacterial strains (S. pyogenes, A. baumannii, E. faecalis, S. aureus, E. coli, K. pneumoniae, and P. aeruginosa), mostly with relatively less potent hemolysis, indicating their possible usefulness as lead structures for the development of new anti-infective agents. It is worth noting that these South American snake venom peptides are comparable in cytotoxicity (e.g., hemolysis) to human cathelicidin LL-37, and much lower than other membrane-active peptides such as mastoparan 7 and melittin from bee venom. Overall, the excellent bactericidal profile of vipericidins suggests they are a promising template for the development of broad-spectrum peptide antibiotics.

  2. Estabilidade e estudo de penetração cutânea in vitro da rutina veiculada em uma emulsão cosmética através de um modelo de biomembrana alternativo Stability and in vitro penetration study of rutin incorporated in a cosmetic emulsion through an alternative model biomembrane

    Directory of Open Access Journals (Sweden)

    André Rolim Baby

    2008-06-01

    Full Text Available A rutina é empregada como antioxidante e na prevenção da fragilidade capilar. Estudos de penetração in vitro através da pele humana seria a situação ideal, entretanto, há dificuldades de sua obtenção e manutenção de sua viabilidade. Entre os demais modelos de membrana, a muda de pele de cobra se apresenta como estrato córneo puro, fornecendo barreira similar ao humano e é obtida sem a morte do animal. Os objetivos desta pesquisa foram desenvolver e avaliar a estabilidade de uma emulsão cosmética contendo rutina e, como promotor de penetração cutânea, o propilenoglicol; e avaliar a penetração e a retenção cutânea in vitro da referida substância ativa da formulação, empregando um modelo de biomembrana alternativo. A emulsão foi desenvolvida com rutina e propilenoglicol, ambos a 5,0% p/p. Quantificou-se a rutina das emulsões por espectrofotometria a 361,0 nm, método previamente validado. A penetração e retenção cutânea in vitro foram realizadas em células de difusão vertical com muda de pele de cobra de Crotalus durissus, como modelo de biomembrana alternativo, e água destilada e álcool etílico absoluto 99,5% (1:1, como fluido receptor. O experimento foi conduzido em um período de seis horas, a 37,0 ± 0,5 ºC e agitação constante de 300 rpm. Empregou-se o método espectrofotométrico validado a 410,0 nm para a quantificação da rutina após penetração e retenção cutânea. A emulsão não promoveu a penetração cutânea da rutina através da muda de pele de C. durissus, retendo 0,931 ± 0,0391 mg de rutina/mg de muda de pele de cobra. Nas condições de armazenamento a 25,0 ± 2,0 ºC; 5,0 ± 0,5 ºC e 45,0 ± 0,5 ºC, a emulsão apresentou-se quimicamente estável durante 30 dias. De acordo com os resultados, a emulsão não favoreceu a penetração cutânea da rutina, mas apenas sua retenção no estrato córneo de C. durissus, condição considerada estável no período de 30 dias.Rutin is

  3. Contribution of homeopathy to the control of an outbreak of dengue in Macaé, Rio de Janeiro Contribuição da Homeopatia para o controle da epidemia de dengue em Macaé, Rio de Janeiro, Brasil Contribución de la homeopatía para el control de un brote de dengue en Macaé, Río de Janeiro,

    Directory of Open Access Journals (Sweden)

    Laila Aparecida de Souza Nunes

    2008-12-01

    Full Text Available Homeopathy has contributed throughout history to the control and eradication of epidemic diseases. Facing the challenge of controlling an outbreak of dengue, the Secretary of Health of the county of Macaé, Rio de Janeiro, Brazil, in early 2007 carried out a “Homeopathy Campaign against Dengue”. 156,000 doses of homeopathic remedy were freely distributed in April and May 2007 to asymptomatic patients and 129 doses to symptomatic patients treated in outpatient clinics, according to the notion of “epidemic genus”. The remedy used was a homeopathic complex against dengue containing Phosphorus 30cH, Crotalus horridus 30cH and Eupatorium perfoliatum 30cH. The incidence of the disease in the first three months of 2008 fell 93% by comparison to the corresponding period in 2007, whereas in the rest of the State of Rio de Janeiro there was an increase of 128%. While confounding factors were not controlled for, these results suggest that homeopathy may be an effective adjunct in Dengue outbreak prevention. Keywords: Homeopathy; Collective Health; Epidemics; Dengue.   Contribuição da Homeopatia para o controle da epidemia de dengue em Macaé, Rio de Janeiro, Brasil Resumo A Homepatia tem contribuído através da História no controle e erradicação de epidemias. Em face ao desafio de controlar uma epidemia de dengue, a Secretaria de Saúde do município de Macaé, Rio de Janeiro, Brasil, iniciou em 2007 a “Campanha da Homeopatia contra a Dengue”. 156,000 doses de medicamento foram gratuitamente distribuídas entre Abril e Maio de 2007 para pacientes assintomáticos e 129 doses para pacientes que já apresentavam os sintomas. Seguindo o conceito de “Gênio Epidêmico” foi usado um complexo homeopático contendo Phosphorus 30c

  4. Homeopathy and Collective Health: The Case of Dengue Epidemics Homeopatia y Salud Coletiva: el caso de la epidemia de Dengue. Homeopatia e Saúde Coletiva: o caso da epidemia de Dengue

    Directory of Open Access Journals (Sweden)

    Renan Marino

    2008-12-01

    Full Text Available This paper describes experiences of the use of homeopathy in the prevention and treatment Dengue fever in São José do Rio Preto, São Paulo, Brazil. May 2001, a single dose of the homeopathic remedy Eupatorium perfoliatum 30cH was given to 40% of residents of the most highly affected neighborhood. Thereafter, Dengue incidence decreased by 81.5%, a highly significant decrease as compared with neighborhoods that did not receive homeopathic prophylaxis (p lower 0.0001. Between April and September 2007, a homeopathic complex composed of Eupatorium perfoliatum, Phosphorus and Crotalus horridus 30cH, given to 20,000 city residents. This trial was aborted prematurely due to national political intervention; therefore, only partial and isolated data could be recorded. However, the results suggest that homeopathy may be effective in the prevention and treatment of Dengue epidemics. Keywords: Homeopathy; public health; Epidemic; Dengue.   Homeopatia e Saúde Coletiva: o caso da epidemia de Dengue Resumo Este artigo descreve a prescrição do tratamento homeopático na prevenção e tratamento da Dengue, na cidade de São José do Rio Preto, São Paulo, Brasil. Em Maio de 2001, uma dose única do medicamento Eupatorium perfoliatum 30 cH foi adminstrado a 40% dos moradores de uma das regiões mais afetadas pelo vírus. Observou-se uma redução de 81,5% na incidência da Dengue, resultado altamente significativo quando comparado com as regiões vizinhas que não receberam o tratamento homeopático (p menor 0.0001. Entre Abril e Setembro de 2007, foi administrado um complexo homeopático composto de Eupatorium perfoliatum, Phosphorus and Crotalus horridus 30cH, para 20.000 moradores. O tratamento foi interrompido prematuramente devido à intervenção de órgãos oficial da Saúde. Em

  5. Revascularização do miocárdio em pacientes após a oitava década de vida

    Directory of Open Access Journals (Sweden)

    ALMEIDA Rui Manuel Sequeira de

    2002-01-01

    Full Text Available OBJETIVO: Avaliar a morbi-mortalidade hospitalar e a qualidade de vida de pacientes, acima de 70 anos de vida, submetidos à operação de revascularização do miocárdio (CRM. CASUÍSTICA E MÉTODOS: No período de julho de 1992 a fevereiro de 2000, foram realizadas 507 CRM, no Serviço de Cirurgia Cardiovascular do Instituto de Moléstias Cardiovasculares de Cascavel. Em 70 destes casos os pacientes tinham idade igual ou superior a 70 anos. Neste grupo predominou o sexo masculino, em 57% dos casos, e a idade média foi de 72,9 anos (70-85 anos. Vinte e seis pacientes apresentavam hipertensão arterial sistêmica, 25 doença pulmonar obstrutiva crônica importante, 17 diabete melito e 8 insuficiência renal crônica, no pré-operatório. Trinta e sete pacientes apresentavam infarto agudo do miocárdio (IAM pré-operatório, sete haviam sido submetidos à angioplastia transluminal percutânea, sete apresentavam lesão de tronco de artéria coronária esquerda e um havia sido submetido à CRM anteriormente. Foram realizados 2,8 enxertos/ paciente, sendo usados condutos arteriais em 53% dos casos. Foi realizada endarterectomia em sete artérias, aneurismectomia de ventrículo esquerdo em sete pacientes e ventriculectomia parcial esquerda em um. A operação foi realizada em caráter de emergência em nove casos. Houve necessidade de contrapulsação aórtica em quatro pacientes. RESULTADOS: O tempo médio de permanência na UTI e no hospital foi de 4 (1-24 e 12,2 (3-34 dias, respectivamente. A mortalidade hospitalar geral foi de 7,1%. Quando analisada por subgrupos, a mortalidade dos pacientes de 70 a 74 anos (57 casos foi de 5,3%, e a dos últimos 35 casos de 2,8%. No pós-operatório imediato, as complicações mais freqüentes foram: insuficiência respiratória (10, arritmia atrial (7, alteração de conduta (6, infecção pulmonar (6, embolia pulmonar (5, síndrome de baixo débito (4, IAM (3, AVC (3, insuficiência renal aguda (4 e

  6. CONSUMO DE LEITE DE VACA E DERIVADOS ENTRE AGRICULTORES DA REGIÃO OESTE DO PARANÁ

    Directory of Open Access Journals (Sweden)

    Heloísa TOMBINI

    2012-04-01

    Full Text Available O leite é a principal fonte de cálcio na alimentação humana, cuja importância está relacionada às suas funções no organismo humano, pois contribui para a formação do tecido ósseo, promove o crescimento, regula o sistema nervoso e aumenta a resistência a infecções. Seus principais nutrientes são: proteínas de alta qualidade, gorduras, carboidratos, além de ser rico em vitaminas e minerais. O objetivo deste estudo foi identificar o consumo de leite de vaca e derivados na população rural residente na região oeste do Paraná. O estudo transversal descritivo foi realizado durante o 20º Show Rural Coopavel, no ano de 2009, em Cascavel, Paraná, com agricultores moradores das áreas urbana e rural dessa região. Para verificar os hábitos alimentares foi aplicado o teste “Como está a sua Alimentação?” validado pelo Ministério da Saúde, cuja classificação segue as porções diárias de leite e derivados recomendadas pela pirâmide alimentar para a população adulta. Dos 372 agricultores que participaram do teste, 193 responderam sobre o consumo de leite, e destes, 160 afirmaram consumir leite e derivados, dos quais 82,5% apresentaram consumo maior ou igual à recomendação e 17,5% apresentaram consumo menor que a recomendação. Em relação à adequação de consumo, 82,7% das mulheres e 82,2% dos homens consomem leite e derivados dentro das recomendações estabelecidas pela pirâmide alimentar, assim como 81,6% dos adultos e 89,5% dos idosos. Segundo as classes econômicas, o consumo adequado de leite e derivados foi verificado em 85,7% na classe A, 90,2% na classe B, 77,5% na classe C e 83,3% na classe D+E, não havendo diferença significativa entre estas variáveis. O tipo de leite mais consumido pelos agricultores foi o leite integral (86,1% e apenas 13,9% relataram ingerir leite desnatado. De acordo com o local de domicílio 20% residia na zona urbana e 80% no meio rural, sendo que dos residentes na cidade, 90

  7. Milk line as an indicator of the harvesting time of three hybrid seeds of corn (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Célio Trzeciak dos Santos

    2005-03-01

    Full Text Available The experiment was carried out to evaluate the time for harvesting seeds of three hybrids of corn (Zea mays L. at their best physiological quality and the use of the milk line as an indicator of the physiological seed maturity. Single hybrids CD 1723 and CD 5501 and double hybrid OC 705 seeds were collected for 59 days, every four days, starting 23 days after female flowering. Seed dry weight, moisture content, germination, vigour (cold and accelerated aging tests, black layer formation and seed milk line development were analysed. The harvesting time started 47 days after female flowering with high physiological quality of the seeds identified by a joint analysis of those seven characteristics of them. The milk line at stage 4 proved to be the best indicator of the time to harvest corn seeds for maximum physiological quality, because different stages of milk line development in corn seeds could be easily identified in the filed without any special equipment.O experimento foi conduzido durante o ano agrícola de 1996/97 em área experimental da Cooperativa de Desenvolvimento Econômico e Tecnológico (Coodetec, em Cascavel, Paraná, com o objetivo de avaliar o efeito da época de colheita de três híbridos de milho (Zea mays L. na qualidade fisiológica das sementes e o uso da linha de solidificação do endosperma como indicativo da maturidade fisiológica das sementes. O plantio foi realizado em 30 de setembro de 1996. Amostras de sementes dos híbridos simples CD 1723 e CD 5501, bem como do híbrido duplo OC 705, foram colhidas em intervalos de quatro dias, durante 59 dias, iniciando-se a colheita no vigésimo terceiro dia após o florescimento feminino. As características avaliadas nas sementes foram o acúmulo de matéria seca, o conteúdo de umidade, a germinação, o vigor (pelos testes de frio e de envelhecimento acelerado, a formação da camada preta e o desenvolvimento da linha de solidificação do endosperma. A colheita dos tr

  8. Animal-based medicines used in ethnoveterinary practices in the semi-arid region of Northeastern Brazil

    Directory of Open Access Journals (Sweden)

    Wedson M.S. Souto

    2012-09-01

    principais espécies citadas foram: o carneiro - Ovis aries (UV=0,89, a raposa - Cerdocyon thous (UV=0,79, o camaleão - Iguana iguana (UV=0,79 e a cascavel - Caudisona durissa (Linnaeus, 1758 (UV=0,74. Os tratamentos mais freqüentemente citados relacionaram-se a doenças inflamatórias, dermatológicas e desordens obstetrícias. Semelhante a outros estudos, a etnoveterinária local estabelece conexões com a etnomedicina para humanos. Os resultados sugerem que similaridades no repertório de recursos medicinais escolhidos pelos moradores locais refletem a acessibilidade/disponbilidade dos recursos. Nosso resultados ajudam a preservar o conhecimento etnoveterinário, o qual é importante para aumentar nossa compreensão das relações entre seres humanos, a sociedades e a natureza, e também elaborar estratégias mais efetivas para conservar os recursos naturais. Outros estudos para validação científica da eficácia e efeitos colaterais destes produtos zooterapêuticos são necessários antes que possam ser recomendados ou não para o uso.

  9. Construction of concept maps as tool for Biochemistry learning

    Directory of Open Access Journals (Sweden)

    Silvia Lopes de Menezes

    2006-07-01

    Full Text Available The use of concept maps on the teaching of sciences has been object of worldwide research with different purposes: to detect the previous knowledge of the students on certain topics or to evaluate learning, among others. Based on Ausubel´s cognitive psychology, concept maps assume that the learning is accomplished by assimilation of new concepts and propositions to the students´ cognitive structure, contributing to establish links between the previous and new knowledge. It is especially interesting on the approach of interdisciplinary issues, as many studied in Biochemistry.The relevance of the use of concept maps on biochemistry learning was evaluated on a thirty-hour undergraduation optional course, with interdisciplinary topics, which are not usually included on introductory Biochemistry courses. The course Biochemistry of Animal Venoms was structured in seven module where the biochemical action mechanisms of the venoms of Crotalus sp (south american rattlesnake, Bothrops sp (jararaca, Loxosceles sp (brown spider, Tityus sp (yellow scorpion, Phoneutria sp (armed spider, Apis mellifera (honey bee and Latrodectus sp (black widowwere discussed. The students worked in small groups and, at each module, there were (1 an oriented study, guided by questions, texts and schemes, supervised by the teachers, (2 the construction of individual concept maps, where the local and systemic effects of the venoms should be predicted by their biochemical composition and (3 the construction of a new map by the group, incorporating the information of the individual maps. The difficulty level of these tasks was gradually increased throughout the course, with lesser time to carry out the tasks, lesser assistance during the oriented study and even lesser information on the venom effects.The course assessment was given by the number, quality and correction of the concepts relationship present in the concept maps, through a questionnaire and by the

  10. Profiling the venom gland transcriptomes of Costa Rican snakes by 454 pyrosequencing

    Directory of Open Access Journals (Sweden)

    Sanz Libia

    2011-05-01

    Full Text Available Abstract Background A long term research goal of venomics, of applied importance for improving current antivenom therapy, but also for drug discovery, is to understand the pharmacological potential of venoms. Individually or combined, proteomic and transcriptomic studies have demonstrated their feasibility to explore in depth the molecular diversity of venoms. In the absence of genome sequence, transcriptomes represent also valuable searchable databases for proteomic projects. Results The venom gland transcriptomes of 8 Costa Rican taxa from 5 genera (Crotalus, Bothrops, Atropoides, Cerrophidion, and Bothriechis of pitvipers were investigated using high-throughput 454 pyrosequencing. 100,394 out of 330,010 masked reads produced significant hits in the available databases. 5.165,220 nucleotides (8.27% were masked by RepeatMasker, the vast majority of which corresponding to class I (retroelements and class II (DNA transposons mobile elements. BLAST hits included 79,991 matches to entries of the taxonomic suborder Serpentes, of which 62,433 displayed similarity to documented venom proteins. Strong discrepancies between the transcriptome-computed and the proteome-gathered toxin compositions were obvious at first sight. Although the reasons underlaying this discrepancy are elusive, since no clear trend within or between species is apparent, the data indicate that individual mRNA species may be translationally controlled in a species-dependent manner. The minimum number of genes from each toxin family transcribed into the venom gland transcriptome of each species was calculated from multiple alignments of reads matched to a full-length reference sequence of each toxin family. Reads encoding ORF regions of Kazal-type inhibitor-like proteins were uniquely found in Bothriechis schlegelii and B. lateralis transcriptomes, suggesting a genus-specific recruitment event during the early-Middle Miocene. A transcriptome-based cladogram supports the large

  11. Biochemical and pharmacological studies of native and irradiated crotamine with gamma radiation of {sup 60}Co; Estudo bioquimico e farmacologico das crotaminas nativa e irradiada com radiacao gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Mitake, Malvina Boni

    2000-07-01

    Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide from the South American rattlesnake venom, composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of this thesis was carry out biochemical and pharmacological studies of native and irradiated crotamine with {sup 60} Co. Crotamine was purified from Crotalus durissus terrificus venom by Sephadex G-100 gel filtration followed by ion exchange chromatography, using a Fast Performance Liquid Chromatography (FPLC) system. It was irradiated at 2 mg/ml in 0.15 M Na Cl with 2.0 kGy gamma radiation emitted by a {sup 60} Co source. The native and irradiated crotamine were evaluated by biochemical characterization, toxic activity (LD{sub 50} and biodistribution. The native and irradiated crotamine were labelled with 29.6 MBq of {sup 125} I using chloramine T method, and separated in a Sephadex G-50 column. Male Swiss mice (35{+-} 5 g), were injected i.p. with o.1 mL (2.4 x 10{sup 6} cpm/mouse) of {sup 125} I native crotamine or with 0.4 mL (1.3 x 10{sup 6} cpm/mouse) of {sup 125} I irradiated crotamine. At 0.08; 0.25; 0.5; 1; 2; 4; 8; 12 and 24 hours the animal were killed by ether inhalation. Blood, spleen, liver, kidneys, brain, lungs, heart, and skeletal muscle were collected in order to determine radioactivity content. The results showed that gamma radiation did not change the protein concentration, the electroforetic profile or the primary structure of the protein, although differences were shown by spectroscopic techniques. The gamma radiation diminished the toxicity of crotamine, but it did not abolish bioactivity. Biodistribution studies showed that native and irradiated crotamine have hepatic metabolism and renal elimination. The native and irradiated crotamine have affinity by skeletal

  12. Acidente ofídico no estado de Goiás

    Directory of Open Access Journals (Sweden)

    Pinho Fábia Maria Oliveira

    2004-01-01

    Full Text Available OBJETIVO: Avaliar aspectos epidemiológicos dos acidentes por serpentes peçonhentas ocorridos no Estado de Goiás. MÉTODOS: Foram analisadas ''Fichas de investigação de acidentes por animais peçonhentos'', pertencentes ao sistema de notificação da Secretaria de Saúde do Estado de Goiás, no triênio 1998-2000. RESULTADOS: Foram notificados, neste período, 3.261 acidentes por serpentes peçonhentas, com coeficiente de incidência variando entre 20 e 23/100.000 habitantes. O maior número de casos ocorreu entre os meses de outubro e abril. Dentre os 2.350 casos em que houve referência ao gênero da serpente, 78,6% foram causados por Bothrops, 20,8% por Crotalus e 6% por Micrurus. Houve predominância do sexo masculino (78,5% e com faixa etária entre 20 e 39 anos de idade. As regiões anatômicas mais freqüentemente picadas foram: pé (43,6%, pernas (23,2% e mãos (20,1%. Em relação ao tempo de atendimento, mais de 80% dos envenenamentos foram atendidos com menos de 6 horas da picada. Os envenenamentos foram classificados, de acordo com a gravidade, em leves (31,6%, moderados (47,5% ou graves (9,6%. As complicações mais comuns foram necrose tecidual no local da picada (31,8% nos envenenamentos botrópicos e insuficiência renal aguda (1,2% nos envenenamentos crotálicos. A letalidade geral foi de 0,46%, sendo a maior taxa observada entre acidentes crotálicos (1%. CONCLUSÕES: Acidentes por serpentes peçonhentas no Estado de Goiás acometem principalmente a população jovem do sexo masculino e têm sido causa de óbito.

  13. Use of I-131- CRTX for targeting malignant adenocarcinoma in mice: biodistribution and radiation dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Raquel Gouvea dos; Soares, Marcella Araugio; Andrade, Henrique Martins de, E-mail: santosr@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Santos, Marcos Antonio da Cunha [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Departamento de Estatistica

    2008-07-01

    Snake venoms molecules have been shown to play a role not only in the survival and proliferation of tumour cells but also in the processes of tumour cell adhesion, migration and angiogenesis. We have shown that {sup 125}I-Crtx, a radiolabeled version of a protein derived from Crotalus durissus terrificus snake venom (Cdt), specifically binds to tumor and triggers apoptotic death. This study reports the biokinetic profile of {sup 99m}Tc-Cdt and {sup 125/131}I-Crtx in Swiss mice bearing Ehrlich solid tumor and MIRD formulation was applied to calculate the absorbed radiation doses for various organs and tumor site. Biokinetic evaluations were performed up to 24 h after intravenous (i.v) or intratumor (i.tu.) injection of {sup 99m}Tc-Cdt or {sup 125/131}I-Crtx. Time-activity curves were generated for the main organs by fitting the organ specific mass mean counts. The radiation dose from {sup 131}I-Crtx was calculated based on non penetrating radiation in the mouse model. Biokinetics data from {sup 99m}Tc-Cdt after i.v. injection in mice tumor model showed rapid blood clearance (T{sub 1/2}= 36.1 ± 2.4 min.), slow tumor clearance (T{sub 1/2}: 108.3 ± 19.5 min.) and indicated the kidneys as the main excretion pathway. Interaction studies in vitro demonstrated that {sup 125}I-Crtx recognize specific sites on Erlich tumor cell membrane. Upon intravenous and intratumor administration of {sup 131}I-Crtx in mice bearing Erlich tumor, it was observed high uptake in tumor site in vivo (Ã =72kBq x h/g) resulting in a high absorbed dose radiation to tumor site. Distributions of {sup 125/131}I-Crtx i.v. were only significant in tumor, stomach, liver and kidneys, reflecting non-specific uptake of Crtx in normal excretion tissues in vivo. Intratumoral administration reduced significantly the radiation dose to the kidneys (42-fold lower) and increased the uptake by the tumor site (128- fold higher). {sup 131}I control was run in a parallel experiment and showed no significant tumor

  14. Obtainment of crotoxin/phema-g-LDPE and crotoxin/PCL systems;Obtencao dos sistemas bioconjugados crotoxina/PEBD-g-phema e crotoxina/PCL

    Energy Technology Data Exchange (ETDEWEB)

    Lorenzetti, Solange Gubbelini

    2006-07-01

    The aim of the work was the obtainment of polymeric matrices immobilized with crotoxin purified from crude venom of rattle snake. A matrix was processed by gamma irradiation by the grafting of a hydrogel onto a polymeric film which resulted in a copolymer for the chemical immobilization of crotoxin. The second matrix was attained by the entrapment of crotoxin in microspheres of epsilon-polycaprolactone. After the purification, the crotoxin proceeding from the snake Crotalus durissus terrificus was evaluated biochemical and biologically. The lethal dose (LD50%) of the toxin was 0.09/kg animal. The test of cytotoxicity not revealed any significant difference between the tumoral cells and the respective normal control cells in culture. Grafting copolymers were used as scaffold for the chemical immobilization of the purified crotoxin. For this purpose the low density polyethylene (LDPE) and the hydrophilic monomer 2-hydroxy-ethyl-methacrylate (HEMA) were copolymerized in a {sup 60}Co source. The copolymers (LDPE-g-PHEMA) showed grafting levels in the range of 2 and 50 %. In the infrared spectroscopy analysis (FTIR-ATR) it was observed in the copolymer, carbonyl groups (C=O) and hydroxyl groups -OH due to the grafting of PHEMA. The MEV micrographs showed a smooth surface for the virgin LDPE and a rough surface for the LDPE-g-PHEMA, owing to the presence of grafted PHEMA. The hydrophilicity was observed by the determination of water content in the copolymer after immersion in water. By the diffusion coefficient it was noted that from 30 % grafting degree, the copolymers become less hydrophilic due to the crosslinking increase among the chains in PHEMA. The biocompatibility of the LDPE-g-PHEMA was proved by the cytotoxicity test. At the end, the immobilized copolymer, the entrapped crotoxin and the free crotoxin was tested 'in vivo'. During 20 days, C3H strain mice were observed in their weight, behavior and motor changes. The results demonstrated that the group

  15. Modulation of the pharmacological effects of enzymatically-active PLA2 by BTL-2, an isolectin isolated from the Bryothamnion triquetrum red alga

    Directory of Open Access Journals (Sweden)

    Nagano Celso S

    2008-06-01

    Full Text Available Abstract Background An interaction between lectins from marine algae and PLA2 from rattlesnake was suggested some years ago. We, herein, studied the effects elicited by a small isolectin (BTL-2, isolated from Bryothamnion triquetrum, on the pharmacological and biological activities of a PLA2 isolated from rattlesnake venom (Crotalus durissus cascavella, to better understand the enzymatic and pharmacological mechanisms of the PLA2 and its complex. Results This PLA2 consisted of 122 amino acids (approximate molecular mass of 14 kDa, its pI was estimated to be 8.3, and its amino acid sequence shared a high degree of similarity with that of other neurotoxic and enzymatically-active PLA2s. BTL-2 had a molecular mass estimated in approximately 9 kDa and was characterized as a basic protein. In addition, BTL-2 did not exhibit any enzymatic activity. The PLA2 and BTL-2 formed a stable heterodimer with a molecular mass of approximately 24–26 kDa, estimated by molecular exclusion HPLC. In the presence of BTL-2, we observed a significant increase in PLA2 activity, 23% higher than that of PLA2 alone. BTL-2 demonstrated an inhibition of 98% in the growth of the Gram-positive bacterial strain, Clavibacter michiganensis michiganensis (Cmm, but only 9.8% inhibition of the Gram-negative bacterial strain, Xanthomonas axonopodis pv passiflorae (Xap. PLA2 decreased bacterial growth by 27.3% and 98.5% for Xap and Cmm, respectively, while incubating these two proteins with PLA2-BTL-2 inhibited their growths by 36.2% for Xap and 98.5% for Cmm. PLA2 significantly induced platelet aggregation in washed platelets, whereas BTL-2 did not induce significant platelet aggregation in any assay. However, BTL-2 significantly inhibited platelet aggregation induced by PLA2. In addition, PLA2 exhibited strong oedematogenic activity, which was decreased in the presence of BTL-2. BTL-2 alone did not induce oedema and did not decrease or abolish the oedema induced by the 48

  16. Involvement of formyl peptide receptors in the stimulatory effect of crotoxin on macrophages co-cultivated with tumour cells.

    Science.gov (United States)

    Costa, E S; Faiad, O J; Landgraf, R G; Ferreira, A K; Brigatte, P; Curi, R; Cury, Y; Sampaio, S C

    2013-11-01

    Crotoxin (CTX) is the main neurotoxic component of Crotalus durissus terrificus snake venom. It inhibits tumour growth and modulates the function of macrophages, which are essential cells in the tumour microenvironment. The present study investigated the effect of CTX on the secretory activity of monocultured macrophages and macrophages co-cultivated with LLC-WRC 256 cells. The effect of the macrophage secretory activities on tumour cell proliferation was also evaluated. Macrophages pre-treated with CTX (0.3 μg/mL) for 2 h were co-cultivated with LLC-WRC 256 cells, and the secretory activity of the macrophages was determined after 12, 24 and 48 h. The co-cultivation of CTX-treated macrophages with the tumour cells caused a 20% reduction in tumour cell proliferation. The production of both H2O2 and NO was increased by 41% and 29% after 24 or 48 h of co-cultivation, respectively, compared to the values for the co-cultures of macrophages of control. The level of secreted IL-1β increased by 3.7- and 3.2-fold after 12 h and 24 h of co-cultivation, respectively. Moreover, an increased level of LXA4 (25%) was observed after 24 h of co-cultivation, and a 2.3- and 2.1-fold increased level of 15-epi-LXA4 was observed after 24 h and 48 h, respectively. Boc-2, a selective antagonist of formyl peptide receptors, blocked both the stimulatory effect of CTX on the macrophage secretory activity and the inhibitory effect of these cells on tumour cell proliferation. Taken together, these results indicate that CTX enhanced the secretory activity of macrophages, which may contribute to the antitumour activity of these cells, and that activation of formyl peptide receptors appears to play a major role in this effect.

  17. Quadro clínico-patológico do envenenamento crotálico experimental em bubalinos comparado com o de bovinos

    Directory of Open Access Journals (Sweden)

    José Diomedes Barbosa

    2011-11-01

    Full Text Available O estudo teve por objetivo verificar a sensibilidade dos bubalinos à peçonha de Crotalus durissus terriiicus, estudar o quadro clínico-patológico e laboratorial nessa espécie e estabelecer comparações com o verificado em bovinos. A inoculação do veneno liofilizado de Crotalus durissus terriiicus, diluído em 1ml de solução fisiológica, foi feita na região da articulação úmero-rádio-ulnar, por via subcutânea, em três bubalinos (doses de 0,015; 0,03; e 0,066mg/kg e em dois bovinos (doses de 0,03 e 0,066mg/kg. O bubalino que recebeu a dose de 0,03mg/ kg apresentou sinais clínicos graves recuperou-se seis dias após, e o bovino que recebeu a mesma dose morreu com evolução de 22h56min. A dose de 0,066mg/kg causou a morte tanto do bovino quanto do bubalino, com evolução clínica de 4h23min e 8h12min, respectivamente. O bubalino que recebeu a dose de 0,015mg/kg, recuperou-se com evolução de 48 horas. Os sinais clínicos tiveram início dentro de 3h58min no bubalino que morreu, e nos bubalinos que adoeceram mas se recuperaram, dentro de 17h25min e 24h00min após a inoculação do veneno. Nos dois bovinos que morreram (com doses de 0,03 e 0,066mg/kg, os primeiros sinais clínicos foram observados 6h10min e 6h31min após a inoculação do veneno. A inoculação do veneno produziu nos búfalos e bovinos um quadro nervoso de paralisia flácida. Os principais sinais observados tanto nos búfalos quanto nos bovinos, foram discreto aumento de volume no local da inoculação, dificuldade respiratória caracterizada por respiração predominantemente abdominal, apatia, sialorreia, dificuldade para se levantar quando estimulados, evolução para decúbito esternal permanente, seguido de decúbito lateral e movimentos de pedalagem, e diminuição dos reflexos relacionados aos pares de nervos cranianos. Nos bubalinos adicionalmente foi observado aumento da base de sustentação, arrastar das pinças dos membros posteriores, marcha lenta e

  18. Aspectos clínico-patológicos da intoxicação experimental pelas sementes de Crotalaria mucronata (Fabaceae em bovinos Experimental poisoning by Crotalaria mucronata (Fabaceae seeds in cattle

    Directory of Open Access Journals (Sweden)

    Murilo R. Boghossian

    2007-04-01

    Full Text Available Sementes moídas de Crotalaria mucronata Desv. (=Crotalaria striata DC., Crotalaria pallida Ait., Crotalaria saltiana, com nomes populares de "xique-xique" ou "guizo-de-cascavel", foram administradas por via oral a 9 bovinos. As doses diárias de 1g/kg, 2g/kg, 3g/kg cada uma a um bovino, e 5g/kg em dois de três bovinos, dadas durante 61 a 63 dias, não causaram intoxicação. A dose de 5g/kg em um bovino, 7,5g/kg em dois bovinos e 10g/kg em um bovino, dadas durante 47-61 dias, causaram sintomas entre 47 e 80 dias após o início da administração e a morte entre 3 horas e 5 dias após o início dos sintomas. Os principais sintomas foram pulso venoso positivo da veia jugular, respiração abdominal, taquicardia, inapetência, fezes ressequidas, edema sub-mandibular e fraqueza. Os achados de necropsia foram palidez pulmonar, hidropericárdio, hidrotórax, hidroperitôneo, edema de mesentério, aumento da consistência hepática, alterações de cor do fígado, dilatação de ventrículo cardíaco direito e edema da parede ruminal. As principais alterações histológicas concentraram-se nos pulmões, sob forma de espessamento das paredes alveolares e da parede das arteríolas com diminuição da luz e fibrose periarteriolar; havia também lesões hepáticas e cardíacas de menor importância. Pode-se concluir que as principais lesões causadas pela ingestão das sementes de C. mucronata durante períodos prolongados, devem-se à dificuldade de passagem do sangue pelos vasos pulmonares em função da hipertensão arterial decorrente de fibrose e espessamento arteriolar determinada pela ação pneumotóxica da planta.Experiments were performed to define the clinical and pathological picture of prolonged administration of the seeds of Crotalaria mucronata Desv. to cattle, in order to obtain additional information about this toxicosis. The ground seeds were administered orally to 9 bovines. Doses of 1g/kg/day, 2g/kg/day, 3g/kg/day each in one bovine

  19. Aspectos clínico-patológicos e laboratoriais do envenenamento crotálico experimental em equinos

    Directory of Open Access Journals (Sweden)

    Cinthia Távora A. Lopes

    2012-09-01

    Full Text Available Descrevem-se os quadros clínico-patológicos e laboratoriais de equinos inoculados experimentalmente com a peçonha de Caudisona durissa terrificus (Crotalus durissus terrificus na antiga nomenclatura, com a finalidade de fornecer subsídios que favoreçam a compreensão desse tipo de acidente ofídico em equinos. O veneno liofilizado foi diluído em 1ml de solução salina a 0,9% e inoculado por via subcutânea em cinco equinos, nas doses de 0,12mg/kg (um animal, 0,066mg/kg (dois animais e 0,03mg/kg (dois animais. O veneno causou a morte do equino que recebeu a dose de 0,12mg/kg e de um dos dois que receberam a dose de 0,066mg/kg, com evolução de 27h27min e 52h29min, respectivamente. O segundo animal que recebeu a dose de 0,066mg/kg também adoeceu, mas recuperou-se após 12 dias da inoculação. A dose de 0,03mg/kg determinou quadros não fatais do envenenamento, com período de evolução que variou entre 6 e 10 dias. O quadro clínico caracterizou-se por considerável aumento de volume no local de inoculação (escápula que se estendeu por todo o membro, apatia e cabeça baixa, alterações locomotoras evidenciadas pelo arrastar das pinças no solo, decúbito e dificuldade para levantar, redução dos reflexos auricular, palatal, do lábio superior e de ameaça, e aumento das frequências cardíaca e respiratória. Os exames laboratoriais revelaram leucocitose por neutrofilia e linfocitose em apenas dois animais. Houve aumento das enzimas creatina quinase (CK, dehidrogenase láctica (DHL e da ureia, e também redução nos níveis séricos de cálcio, fósforo e magnésio. O tempo de tromboplastina parcial ativada (TTPA aumentou nos equinos que morreram. Os achados de necropsia foram edema do tecido subcutâneo em todo o membro em que foi aplicado o veneno, sufusões no epicárdio dos ventrículos cardíacos esquerdo e direito, e bexiga com áreas hemorrágicas em grande parte da mucosa. Ao exame histopatológico observaram-se f

  20. Study of crotoxin mechanism of action to mammary carcinomas and evaluation of its potential as a radiopharmaceutical; Estudo do mecanismo de acao da crotoxina em tumores mamarios e avaliacao do seu potenctial radiofarmaceutico

    Energy Technology Data Exchange (ETDEWEB)

    Silveira, Marina Bicalho

    2010-07-01

    Crotoxin, the main component of Crotalus durissus terrificus snake venom, has been studied since 1938. It is a natural polypeptidic complex with pharmacological potential because of its antitumoral properties which has attracted great interest for diagnosis and therapy of oncological diseases. However, Crotoxin mechanism of action and sites of specific interaction on tumor cells are still misunderstood. Breast cancer is the second most frequent type in the world and the most common cancer in women. About 30 to 60% of mammary tumors overexpress epidermal growth factor receptor (EGFR), a transmembrane protein related to cell proliferation. Since literature has reported that Crotoxin antitumoral effect is more potent on cells with EGFR overexpression the objectives of this work were to evaluate Crotoxin cytotoxic effects on mammary tumor cells human breast carcinoma (MCF-7) and Ehrlich tumor cells (murine ascitics carcinoma), and to investigate the specific molecular interaction of Crotoxin on Ehrlich tumor cells. Initially, Crotoxin was radiolabelled with iodine-125 ({sup 125}I-Crotoxin) and iodine-131 ({sup 131}I-Crotoxin). Saturation and competition assay were carried out to characterize Crotoxin in vitro interaction; Crotoxin biodistribution studies and singlephoton emission computed tomography (SPECT) of mice bearing Ehrlich tumor have been evaluated to describe in vivo interaction. Our results showed that Crotoxin presented cytotoxic effect against Ehrlich with DL{sub 50} in vitro (concentration of compound which is lethal for 50% of cells) of about one micromolar, but did not present significant effect against MCF-7. Morphological alterations characteristic of apoptosis suggests programmed cell death. {sup 125}I-Crotoxin interaction with Ehrlich tumor cells was saturable with approximately 70% specificity, and presented K{sub d}=24.98 nmol/L and B{sub max}=16,570 sites/cell for low affinity binding sites and K{sub d}=0.06 nmol/L and B{sub max}=210 sites

  1. Ecological Monitoring and Compliance Program 2014 Report

    Energy Technology Data Exchange (ETDEWEB)

    Hall, Derek B. [National Security Technologies, LLC, Las Vegas, Nevada (United States); Anderson, David C. [National Security Technologies, LLC, Las Vegas, Nevada (United States); Greger, Paul D. [National Security Technologies, LLC, Las Vegas, Nevada (United States); Ostler, W. Kent [National Security Technologies, LLC, Las Vegas, Nevada (United States)

    2015-05-12

    The Ecological Monitoring and Compliance Program (EMAC), funded through the U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO, formerly Nevada Site Office), monitors the ecosystem of the Nevada National Security Site (NNSS) and ensures compliance with laws and regulations pertaining to NNSS biota. This report summarizes the program’s activities conducted by National Security Technologies, LLC (NSTec), during calendar year 2014. Program activities included (a) biological surveys at proposed activity sites, (b) desert tortoise compliance, (c) ecosystem monitoring, (d) sensitive plant species monitoring, (e) sensitive and protected/regulated animal monitoring, and (f) habitat restoration monitoring. During 2014, all applicable laws, regulations, and permit requirements were met, enabling EMAC to achieve its intended goals and objectives. Sensitive and protected/regulated species of the NNSS include 42 plants, 1 mollusk, 2 reptiles, 236 birds, and 27 mammals. These species are protected, regulated, or considered sensitive according to state or federal regulations and natural resource agencies and organizations. The desert tortoise (Gopherus agassizii) and the western yellow-billed cuckoo (Coccyzus americanus) are the only species on the NNSS protected under the Endangered Species Act, both listed as threatened. However, only one record of the cuckoo has ever been documented on the NNSS, and there is no good habitat for this species on the NNSS. It is considered a rare migrant. Biological surveys for the presence of sensitive and protected/regulated species and important biological resources on which they depend were conducted for 18 projects. A total of 199.18 hectares (ha) was surveyed for these projects. Sensitive and protected/regulated species and important biological resources found during these surveys included a predator burrow, one sidewinder rattlesnake (Crotalus cerastes), two mating speckled rattlesnakes

  2. Infrared Sensory System in the Crotaline Snake:a Possible Experimental Model for Chinese Qigong Approach%蛇类红外线感受系统:一种可能的外气功研究实验动物模型

    Institute of Scientific and Technical Information of China (English)

    梁运飞

    2006-01-01

    许多蛇类,例如响尾蛇属,洞蛇属,饭匙倩蛇属,竹叶青蛇属和蝮蛇属等在头部具有一对能在黑暗中探测和捕获猎物的凹陷器官.这种凹陷器官对红外射线非常敏感,因此也称为红外线感受器官.凹陷器官在中间部位被一层约为15μm厚的薄膜(红外线感受膜)分隔为外腔和内腔,红外线感受膜由三叉神经节中的特化假单极神经细胞(红外线感受细胞)的外周轴突所支配,红外线感受膜内相邻的游离神经末梢聚合形成约40μm直径的团块,构成了基本的红外线感受野单元.三叉神经节中的红外线感受细胞的中枢轴突投射到同侧延髓中的三叉神经束外侧降核,该神经核团为此类蛇属所特有.从三叉神经束外侧降核二级神经元发出的轴突投射到对侧视顶盖.由于蛇类不具有分化的半球新皮质,因此视顶盖为红外线感受系统的感觉与行为的整合中枢.在三叉神经节,延髓三叉神经束外侧降核及视顶盖均可记录到神经细胞对红外线刺激的反应电位,从而可观察红外线刺激强度与各级红外线感受神经元反应强度的关系.本文简述了蛇类红外线感受系统的形态学和生理学特征及其研究进展,并且探讨了利用蛇类红外线感受系统作为生物体接受外气功研究的实验动物模型的可能性.%Certain of the snakes, such as Crotalus, Bothrops, Lachesis, Trimeresurus, and Agkistrodonpossess a pair of pit organs which enable snakes to detect and capture prey animals in the dark. The pit organs are highly sensitive to infrared ray and so are called infrared organs. Specific cells in the trigeminal ganglion innervate a pit membrane locating at the bottom of pit organs with their free nerve endings which act as infrared detectors. These cells send their central axons ipsilaterally to a specific nucleus located lateral to the trigeminal spinal tract in medulla oblongata that is called as nucleus of lateral

  3. O sofrimento psíquico do profissional de enfermagem

    Directory of Open Access Journals (Sweden)

    Glaudston Silva de Paula

    2012-04-01

    satisfação do profissional. O mesmo culmina na plenitude da realização profissional, em fazer o que gosta, não obstante os dissabores e conflitos no meio.CONCLUSÃODentro da pesquisa que nos propusemos a fazer atingimos o nosso objetivo, onde nossos dados confirmam que o maior sofrimento psíquico está diretamente ligado a organização do trabalho e não com a profissão. É notório que o orgulho de ser enfermeiro se conflita com uma condição de trabalho insatisfatória gerada também pela dificuldade de relacionamento interpessoal.Os resultados nos levam a essência da profissão, que se fundamenta no cuidar com amor ao próximo, com doação e humildade, pois a grande maioria dos entrevistados se consideram plenos, realizados e felizes em sua profissão. Assim, retomamos aos primórdios da profissão, cujas bases foram erigidas por Florence Nightingale galgadas pelo rol do altruísmo. REFERÊNCIAS1.     Baba V, Galaperin BL, Lituchy TR. Occupational mental health: a study of work-related depression among nurses in the Caribbean. International Journal of Nursing Studies. 1999; 36(1: 163-9.2.     Santos MS, Trevizan MA. Sofrimento psíquico no trabalho do enfermeiro. Nursing Rev Téc Enf. 2002; 52(1: 23-28.3.     Moos RH, Cronkite RC, Finney, JW. Health and Daily Living Form Manual. 2ª ed. California: Mind Garden; 1990.4.     Pitta AM. Hospital: dor e morte como ofício. São Paulo: Hucitec; 1990.5.      Minayo MCS. O desafio do conhecimento: pesquisa qualitativa em Saúde. São Paulo: Hucitec; 2004.6.     Dejours, C. Uma nova visão do sofrimento humano nas organizações In: Chanlat, JF. O indivíduo na Organização. 3ª ed. São Paulo: Atlas; 1996.7.     Dejours, C. A loucura do trabalho: estudo de psicopatologia do trabalho. 5ª ed. São Paulo: Cortez; 1992.8.     Milanesi K, Collet N, Viera CS, Oliveira BRG. Sofrimento Psíquico em Dejours. Seminário Nacional: Estado e Políticas Sociais no Brasil. Cascavel: Edunioeste

  4. Structures and Functions Prediction and Expression Profiles of Calreticulin as Calcium Binding Chaperones in Chicken%鸡钙离子结合分子伴侣Calreticulin的结构与功能预测及组织表达特性

    Institute of Scientific and Technical Information of China (English)

    王丽丽; 李楠; 曹嫦妤; 龚都强; 于东; 王伟; 李金龙

    2014-01-01

    内的终端非还原性α-L-阿拉伯呋喃糖苷残基的水解,作用于α-L-阿拉伯呋喃糖苷、含(1,3)和/或(1,5)糖苷键的阿拉伯聚糖、阿拉伯木聚糖和阿拉伯半乳聚糖,能与糖类分子及Ca2+特异性结合,可监控糖蛋白组装折叠及Ca2+调控,且在消化系统中发挥重要作用。%[Objective] The aim of the current study is to reveal the evolutionary relationships, and investigate the protein structure and functions and the expression profiles of calreticulin (CRT) as a key Ca2+ binding molecular chaperone within the endoplasmic reticulum (ER) of chicken.[Method]The nucleotides and amino acids of CRT in 12 species of vertebrates recorded in Gene bank were analyzed for evolutionary relationships by Laser Gene, and the structures and functions of CRT protein in chicken were predicted by bioinformatics, and the expression profiles of CRT in 30 organizations of chicken was analyzed by real-time PCR.[Result]Results of homology analysis showed that compared with the other 11 species of nucleotide sequences of CRT gene in chicken, gallus gallus and oryctolagus cuniculus had the highest nucleotide sequence homology, which was 78.7%, in addition, gallus gallus and oncorhynchus mykiss had the lowest homology, which was 70.5%. In the homology of amino acid sequences, the relationship between gallus gallus and crotalus adamanteus cadam is the closest by 85.0%, and the furthest relationships with gallus gallus is oncorhynchus mykiss which was 69.0% in amino acid sequence, besides, the homology of gallus gallus with cricetulus griseus, macaca mulatta, homo sapiens, oryctolagus cuniculus, sus scrofa, bos taurus, and xenopus (silurana) tropicalisis relatively close to almost above 80.1%. The protein structure and function prediction revealed that the CRT of chicken was constitute with 404 amino acids, and had a relative molecular mass of 46.8802 kD and a theoretical isoelectric point of 4.41, moreover, the negative charge