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Sample records for carp cyprinus carpio

  1. Food handling and mastication in the carp (Cyprinus carpio L.)

    NARCIS (Netherlands)

    Sibbing, F.A.

    1984-01-01

    The process of food handling in the common carp ( Cyprinus carpio L.) and its structures associated with feeding are analyzed. The aim of this study is to explain the relation between the the architecture of the head and its functions in food processing and to determine the specializations for some

  2. Food handling and mastication in the carp (Cyprinus carpio L.)

    OpenAIRE

    Sibbing, F.A.

    1984-01-01

    The process of food handling in the common carp ( Cyprinus carpio L.) and its structures associated with feeding are analyzed. The aim of this study is to explain the relation between the the architecture of the head and its functions in food processing and to determine the specializations for some food types and the consequent restrictions for others. Such information improves our understanding of the trophic interrelations between different fish species living together in one community.- Cy...

  3. Mucosal immune response in common carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Przybylska, Dominika Alicja

    of the biological impact of two commercially available ß-glucan enriched products on the wound healing process in common carp (Cyprinus carpio L.) in sterile, controlled conditions; 2. investigation of potential impact of intravenously injected ß-glucan on mucosal immune response and immunoglobulin......-glucans. Further work showed that bath in two commercially available ß-glucan enriched products, specifically MacroGard and 6.3 kDa oat fiber, had a direct positive effects on the wound closure in common carp and promoted faster wound healing compared to non-treated fish. We showed the immunological and......Control of fish diseases is a great concern in aquaculture because of losses in the production. Drug choices for the treatment of common infectious diseases are becoming increasingly limited and expensive and, in some cases, unavailable due to the emergence of drug resistance in bacteria and fungi...

  4. Genotoxicity monitoring of freshwater environments using caged carp (Cyprinus carpio).

    Science.gov (United States)

    Klobucar, Göran I V; Stambuk, Anamaria; Pavlica, Mirjana; Sertić Perić, Mirela; Kutuzović Hackenberger, Branimir; Hylland, Ketil

    2010-01-01

    The present study deals with genotoxicity assessment of freshwaters using caged carp (Cyprinus carpio). Carps were transplanted from a fish-farm to three differently polluted sites in eastern Croatia. Two polluted sites were situated in the river Drava, downstream from the cities of Belisće and Osijek, while the reference site was in the Nature Park Kopacki rit, a preserved wetland area with limited anthropogenic influence. Exposure lasted for 3 weeks and was repeated for 3 years (2002-2004). DNA damage was assessed in erythrocytes of the exposed animals by the Comet assay and micronucleus test (MNT). In order to evaluate possible differences in stress responses to polluted water in situ and in aquaria a laboratory exposure was performed with water from the studied location in the second year of the study. Carp from the sites with high anthropogenic influence (Belisće and Osijek) had higher average DNA damage as expressed in both the MNT and Comet assay. Of the two, the Comet assay appeared to be more sensitive following both caging and aquaria exposures. The results from this study suggest that 3 weeks caging exposure of C. carpio may be a useful strategy to monitor for genotoxic agents in freshwater ecosystems. PMID:19626438

  5. Hormonal regulation of hepatic glycogenolysis in the carp, Cyprinus carpio

    Energy Technology Data Exchange (ETDEWEB)

    Janssens, P.A.; Lowrey, P.

    1987-04-01

    Carp (Cyprinus carpio) liver maintained normal glycogen content and enzyme complement for several days in organ culture. Epinephrine-stimulated glycogenolysis, phosphorylase activation, and cyclic AMP (cAMP) accumulation in a concentration-dependent manner with EC/sub 50/s of 100, 100, and 500 nM, respectively. These actions were blocked by the ..beta..-adrenergic antagonist, propranolol, but not by the ..cap alpha..-adrenergic antagonist phentolamine. Glycogenolysis and tissue cAMP were uninfluenced by 10/sup -6/ M arginine vasotocin, arginine vasopressin, lysine vasotocin, lysine vasopressin, mesotocin, or oxytocin, but were slightly increased by 10/sup -5/ M isotocin and slightly decreased by 10/sup -6/ M angiotensin II. (/sup 125/I)-iodocyanopindolol (ICP), a ..beta..-adrenergic ligand, bound to isolated carp liver membranes with a K/sub D/ of 83 pM. Maximum binding of 45 fmol/mg protein was at 600 pM. Propranolol, isoprenaline, epinephrine, phenylephrine, norepinephrine, and phenoxybenzamine displaced ICP with K/sub D/s of 100 nM, 2, 20, 20, 60, and 200 ..mu..M, respectively. The ..cap alpha..-adrenergic antagonists, yohimbine and prazosin, showed no specific binding. These data provide evidence that catecholamines act via ..beta..-adrenergic receptors in carp liver and that ..cap alpha..-adrenergic receptors are not present. Vasoactive peptides play no significant role in regulation of carp liver glycogenolysis.

  6. Effects of phorbol esters in carp (Cyprinus carpio L).

    Science.gov (United States)

    Becker, K; Makkar, H P

    1998-04-01

    Carp (Cyprinus carpio L) were fed diets containing phorbol esters at concentrations of 0, 3.75, 7.5, 15, 31, 62.5, 125, 250, 500 and 1,000 micrograms/g feed. Phorbol esters were from Jatropha curcas nuts. Jatropha curcas toxicity has been reported in humans, rodents and livestock, and phorbol esters have been identified as the main toxic agent. The adverse effects observed in carp at phorbol esters concentrations of 31 micrograms/g or higher were lower average metabolic growth rate, fecal mucus production and rejection of feed. Average metabolic growth rates (g/kg 0.8/d) in a 7-d experimental period during which diets containing phorbol esters were fed to carp (values with different letters being significantly different) were 15.4a, 14.4a, 12.5ab, 12.4ab, 10.9b, 3.4c, 0.2c, -3.8d, -4.9d and -5.6d, respectively, at the above mentioned concentrations. The values for the recovery phase of 9-d during which phorbol esters were not included in the diet were 16.0a, 15.6a, 14.9a, 15.6a, 5.3b, 1.6b, 4.6bc, 6.3bc, 7.8c and 8.2c, respectively. The adverse effects of phorbol esters were reversible since withdrawal of the esters from the diets led to gain in body mass. None of the fish died at any of the concentrations studied. Incorporation of vitamin C, an antioxidant, at levels of 0.4 and 2% in the feed did not prevent occurrence of the adverse effects of the phorbol esters. The threshold level at which phorbol esters appeared to cause adverse effects in carp was 15 micrograms/g feed or 15 ppm in the diet. Carp were highly sensitive to phorbol esters, thus making them a useful species for bioassay of these compounds. This bioassay together with other analytic procedures could be of immense use in the development of detoxification processes for agro-industrial products containing phorbol esters, such as jatropha meal or jatropha oil, and as a quality control method to monitor successive stages in industrial detoxification processes. PMID:9554059

  7. Carp (Cyprinus carpio L.) innate immune factors are present before hatching

    NARCIS (Netherlands)

    Huttenhuis, B.T.; Grou, C.P.O.; Taverne-Thiele, J.J.; Taverne, N.; Rombout, J.H.W.M.

    2006-01-01

    Expression of the innate immune factors, complement factor 3 (C3), ¿2-macroglobulin (¿2M), serum amyloid A (SAA) and a complement factor 1 r/s ¿ mannose binding lectin associated serine protease-like molecule (C1/MASP2), was determined with Real Time Quantitative-PCR in carp (Cyprinus carpio L.) ont

  8. Development and application of genetically uniform strains of common carp (Cyprinus carpio L.)

    NARCIS (Netherlands)

    Bongers, A.B.J.

    1997-01-01

    In this thesis, the development of genetically uniform strains of common carp, Cyprinus carpio L. is described. As in research on mammals, the use of genetically uniform fish could increase the quality (replicability, reproducability and repeatability) of experiments. Inbreeding was done by gynogene

  9. Characterization of the stress response in 17a-hydroxylase deficient common carp (Cyprinus carpio L.)

    NARCIS (Netherlands)

    Nematollahi, M.A.

    2008-01-01

    In this thesis we describe interrenal hyperplasia in a teleost fish, the common carp, Cyprinus carpio L, caused by 17α-hydroxylase deficiency. Two homozygous, XX male inbred strains, “E5” and “E7”, consistently show 5-10 fold less plasma cortisol in response to net confinement compared to normal mal

  10. Expression of major histocompatibility complex genes in carp (Cyprinus carpio L.).

    NARCIS (Netherlands)

    Rodrigues, P.N.S.

    1996-01-01

    The common carp ( Cyprinus carpio L.) has been the experimental animal of choice because many features of the immune system of this Cyprinid fish have been well characterized. The immune system consists of an integrated set of organs containing cells such as Ig + 

  11. The effect of replacing fishmeal with Spirulina on microbial load in common carp Cyprinus carpio L

    OpenAIRE

    N.M. Abdulrahman; and H.J.H. Ameen

    2013-01-01

    The use of blue green algae Spirulina in aquaculture has several potential advantages over the production of fish. This study was designed to investigate the effect of different replacement levels of fishmeal with Spirulina on microbial load of common carp Cyprinus carpio L., the trail was conducted for 105 days and for this purpose 200 fingerlings common carp. Mean initial weight was (32.7 g). The fish were acclimated to laboratory conditions and fed with control pellets (32% protein) prior ...

  12. Effects of Dietary Exposure to Zearalenone (ZEN) on Carp (Cyprinus carpio L.)

    OpenAIRE

    Constanze Pietsch; Susanne Kersten; Hana Valenta; Sven Dänicke; Carsten Schulz; Patricia Burkhardt-Holm; Ranka Junge

    2015-01-01

    The mycotoxin zearalenone (ZEN) is frequently contaminating animal feeds including feed used in aquaculture. In the present study, the effects of dietary exposure to ZEN on carp (Cyprinus carpio L.) were investigated. ZEN at three different concentrations (low dose: 332 µg kg−1, medium dose: 621 µg kg−1 and high dose: 797 µg kg−1 final feed, respectively) was administered to juvenile carp for four weeks. Additional groups received the mycotoxin for the same time period but were fed with the u...

  13. Heritability estimates for growth-related traits using microsatellite parentage assignment in juvenile common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Vandeputte, M.; Kocour, Martin; Mauger, S.; Duppont Nivet, M.; De Guerry, D.; Rodina, Marek; Gela, David; Vallod, D.; Chevassus, B.; Linhart, Otomar

    2004-01-01

    Roč. 235, - (2004), s. 223-236. ISSN 0044-8486 Institutional research plan: CEZ:AV0Z5045916 Keywords : common carp * Cyprinus carpio * heritability Subject RIV: ED - Physiology Impact factor: 1.627, year: 2004

  14. Acute toxicity test of pesticide abamectin on common carp (Cyprinus carpio)

    Institute of Scientific and Technical Information of China (English)

    Aliakbar Hedayati; Mohammad Forouhar Vajargah; Ahmad Mohamadi Yalsuyi; Safoura Abarghoei; Michael Hajiahmadyan

    2014-01-01

    Objective: To determine acute toxicity of abamectin (abamectin used for agricultural fields and also is a common acaridae used in farms) to common carp (Cyprinus carpio). Methods: In this research, common carps were exposed to abamectin for 96 h. LC50 values of 24 h, 48 h, 72 h and 96 h were attained by probit analysis software SPSS Version 16. Fish were exposed to different concentrations (1, 2, 3, 6, 12 and 15 mg/L) of abamectin for 96 h and physicochemical properties of water used for these experiments were stable and every mortality was recorded daily. Results:The 96 h LC50 of abamectin for Cyprinus carpio was 1.243 mg/L. Conclusions: Eventually toxicity values indicated that abamectin has same toxicity in studied other specie and we can state lower value of LC50 for studied specie in compare with most species.

  15. Biochemical changes of antioxidant enzymes in common carp (Cyprinus carpio L.) after heavy metal exposure

    OpenAIRE

    Vinodhini, Rajamanickam; Narayanan, Muthuswamy

    2009-01-01

    Accidental industrial spills may lead to a high concentration of toxic metals in the aquatic environment, as well as affecting freshwater ecosystems with acute and chronic toxicity. Of all aquatic species, fish are particularly sensitive to waterborne contamination and are recognized as bioindicators for water quality monitoring. The objective of the present study was to evaluate the biochemical changes of the enzymatic defense systems in fresh water fish, the common carp (Cyprinus carpio L.)...

  16. Afferent and Efferent Connections of the Optic Tectum in the Carp (Cyprinus carpio L.)

    OpenAIRE

    Luiten, P.G.M.

    1981-01-01

    The afferent and efferent connections of the tectum opticum in the carp (Cyprinus carpio L.) were studied with the HRP method. Following iontophoretic peroxidase injections in several parts of the rectum anterograde transport of the enzyme revealed tectal projections to the lateral geniculate nucleus, dorsal tegmentum, pretectal nuclei, nucleus rotundus, torus longitudinalis, torus semicircularis, nucleus isthmi, contralateral tectum and to the mesencephalic and bulbar reticular formations. T...

  17. A History of common carp Cyprinus carpio (L.) in Ireland: A Review

    OpenAIRE

    Brazier, B.; Caffrey, J.M.; Cross, T. F.; Chapman, D. V.

    2012-01-01

    This paper represents the most comprehensive and detailed summary of the history of common carp Cyprinus carpio (L.) in Ireland to date. It charts the earliest known introductions of the species to Irish waters, the rise in popularity of recreational angling for the species from c. 1950 onwards, the work carried out to establish the species in Ireland and explains the primary causes of their more recent distribution increase. Much of the historical research material gathered on...

  18. Influence of music on the growth of koi carp, Cyprinus carpio (Pisces: Cyprindae)

    OpenAIRE

    Vasantha, L.; Jeyakumar, A.; Pitchai, M.A.

    2003-01-01

    An experiment was carried out to investigate the influence of music on the growth of Koi Carp (Cyprinus carpio) by subjecting the fish to music. Weekly growth in weight was recorded and used to calculate the growth rate and specific growth rate. The difference in growth between the control and experiment groups of fishes was statistically tested for significance. It was observed that the growth of fish subjected to music was significantly higher.

  19. Demasculinisation of sexually mature male common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during spermatogenesis

    NARCIS (Netherlands)

    Gimeno, S.; Komen, H.; Jobling, S.; Sumpter, J.; Bowmer, T.

    1998-01-01

    Sexually mature male common carp (Cyprinus carpio) were exposed for a 3-month period to sublethal concentrations of 4-tert-pentylphenol (TPP) and to 17β-estradiol (E2) during spermatogenesis. This was part of a broad research programme investigating the effects of TPP on the life stages of the carp

  20. Antioxidant activity and functional properties of enzymatic protein hydrolysates from common carp (Cyprinus carpio) roe (egg).

    Science.gov (United States)

    Chalamaiah, M; Jyothirmayi, T; Diwan, Prakash V; Dinesh Kumar, B

    2015-09-01

    Previously, we have reported the composition, molecular mass distribution and in vivo immunomodulatory effects of common carp roe protein hydrolysates. In the current study, antioxidative activity and functional properties of common carp (Cyprinus carpio) roe (egg) protein hydrolysates, prepared by pepsin, trypsin and Alcalase, were evaluated. The three hydrolysates showed excellent antioxidant activities in a dose dependent manner in various in vitro models such as 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis(3-ethylbenzthiazoline-6)-sulfonic acid (ABTS(+)) radical scavenging activity, ferric reducing antioxidant power (FRAP) and ferrous ion (Fe(2+)) chelating ability. Enzymatic hydrolysis significantly increased protein solubility of the hydrolysates to above 62 % over a wide pH range (2-12). Carp roe hydrolysates exhibited good foaming and emulsification properties. The results suggest that bioactive carp roe protein hydrolysates (CRPHs) with good functional properties could be useful in health food/nutraceutical/pharmaceutical industry for various applications. PMID:26344996

  1. Minor effect of depletion of resident macrophages from peritoneal cavioty on resistance of common carp Cyprinus carpio to blood flagellates

    OpenAIRE

    Saeij, J.P.J.; Groeneveld, A.; Rooijen, van, J.; Haenen, O. L. M.; Wiegertjes, G. F.

    2003-01-01

    Carp Cyprinus carpio macrophages were depleted by intraperitoneal (i.p.) injection of clodronate-liposomes for the in vivo study of the effect of macrophage depletion on the resistance of carp to infection with blood flagellate parasites. Clodronate released inside the cell induces apoptosis of (murine) macrophages. Following i.p. injection of carp with liposomes alone, but not with Trypanoplasma borreli, neutrophilic granulocytes rapidly migrated from the head kidney to the peritoneal cavity...

  2. The effect of replacing fishmeal with Spirulina on microbial load in common carp Cyprinus carpio L

    Directory of Open Access Journals (Sweden)

    N.M. Abdulrahman

    2013-06-01

    Full Text Available The use of blue green algae Spirulina in aquaculture has several potential advantages over the production of fish. This study was designed to investigate the effect of different replacement levels of fishmeal with Spirulina on microbial load of common carp Cyprinus carpio L., the trail was conducted for 105 days and for this purpose 200 fingerlings common carp. Mean initial weight was (32.7 g. The fish were acclimated to laboratory conditions and fed with control pellets (32% protein prior to the feeding trials for 21 days. Five experimental diets were used and Spirulina replaced fishmeal protein from the standard diet at 0% (T1, 5% (T2, 10% (T3, 15% (T4 and 20% (T5 levels. Bacterial total account in rearing water and the bacterial total count in carp intestine for the third treatment was higher significantly as compared to other treatments, which is the conclusion of this study.

  3. REARING EFFICIENCY AND NUTRITIONAL QUALITY ASSESSMENT FOR CARP SAPLING (CYPRINUS CARPIO LINNE, 1758 FROM RECIRCULATING SYSTEMS

    Directory of Open Access Journals (Sweden)

    ELPIDA PALTENEA

    2013-12-01

    Full Text Available Common carp, Cyprinus carpio L. of 8 months older, rearing in recirculation system during 107 days period - he has been analysed from the point of view of feeding efficiency and nutritional quality of carp carcass. The stocking of breeding ponds has been made at 12.17 kg/m3 density, the initial average weight of fishes has been 82.2 g/ex., biochemical composition of carp carcass has been following: protein 14.35%, fat 1.10%, ash 1.67% and moisture 82.22%. Weekly, there has been analysed biochemical composition of meat and protein efficiency coefficient (PER, protein using efficiency (PUE, retained protein (RP. The nutritional quality of fish meat and appreciation coefficients of fish rearing they indicate a good capitalization of delivered food.

  4. Molecular characterisation of a disseminated Cryptosporidium infection in a Koi carp (Cyprinus carpio).

    Science.gov (United States)

    Yang, Rongchang; Dorrestein, Gerry M; Ryan, Una

    2016-08-15

    Cryptosporidium is a protozoan parasite that infects a wide range of hosts, yet relatively little is known about the epidemiology of cryptosporidiosis in fish. Here we report a disseminated Cryptosporidium infection in a male Koi carp (Cyprinus carpio), with parasite stages identified deep within the epithelium of the intestine, kidneys, spleen, liver and gills causing severe granulomatous inflammatory lesions. Molecular characterization at two loci; 18S ribosomal RNA (rRNA) and actin, revealed this to be a novel Cryptosporidium genotype, most closely related to Cryptosporidium molnari. PMID:27514884

  5. Influence of broodstock age on reproductive performance in koi carp (Cyprinus carpio L.

    Directory of Open Access Journals (Sweden)

    P. Melotti

    2011-03-01

    Full Text Available The koi carp (Cyprinus carpio L. is a very important species in the ornamental fish culture. This fish, in relation to the variety of its colour patterns is very appreciated and it can obtain an higher market price compared to the common goldfish. In the last ten years, breeding goals have increasingly focused their attention to the morphological characteristics (Cherfas et al., 1992 and numerous research works were carried out on pigmentation and viability of different colour phenotypes (Jackson et al., 2000. More recently, several studies were set up in order to produce diploid gynogenotes and triploids (Rothbard et al., 1999...

  6. THE EFFECT OF FISH FEEDING WITH ADDITIVES NUPRO® AND BIO-MOS® ON THE RESULTS OF THE REARING OF AGE-1+ CARP (CYPRINUS CARPIO CARPIO)

    OpenAIRE

    А. Vaschenko; N. Matvienko

    2015-01-01

    Purpose. To investigate the effect of feed fish with the additives NUPRO® and BIO-MOS® on the results of the rearing of age-1+ carp (Cyprinus carpio L.). Methodology. The study of the effect of feeding fish with the additives NUPRO® and BIO-MOS® on the results of the rearing age-1+ carp (Cyprinus carpio L.) of “Nyvka” scaled intrabreed type were performed based on the conventional methodology. The experiments were carried out in ponds condition of the research farm "Nyvka" of the Insti...

  7. Interaction between the blood fluke, Sanguinicola inermis and humoral components of the immune response of carp, Cyprinus carpio

    OpenAIRE

    Roberts, M.L.; Lewis, J W; Wiegertjes, G.F.; Hoole, D.

    2005-01-01

    The effect of Sanguinicola inermis on serum antibody and complement activity in Cyprinus carpio was assessed using an ELISA and haemolytic assays. Possible immune evasion strategies were assessed using immunodetection of host proteins on the surface of the parasite. Carp acclimatized to 20 or 25 °C were infected by exposure to 500 cercariae or injected intraperitoneally with 150 cercariae, and serum monitored over a 63-day period. In cercariae-injected carp, irrespective of time and temperatu...

  8. Effect of heavy metals induced toxicity on metabolic biomarkers in common carp (Cyprinus Carpio L.

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    Vinodhini Rajamanickam

    2008-02-01

    Full Text Available This research paper presents the pathological effects of a sub-lethal concentration of heavy metals (cadmium, lead, nickel, and chromium on common carp (Cyprinus carpio L.. Total protein and levels of alkaline phosphatase (ALP, alanine aminotransferase (ALT, aspartate aminotransferase (AST and lactate dehydrogenase (LDH in the liver tissue were measured. Compared with the control group a significant decrease of total protein (p < 0.001 was ascertained in the experimental group. The ALP on the other hand was significantly higher (p < 0.001. The values of ALT, AST, and LDH significantly decreased in the first day and then progressively increased afterwards (p < 0.001. The above results on the biochemical profile indicate marked hepatotoxic effects of heavy metals in common carp.

  9. Acute effect of copper exposure on serum biochemical characteristics of common carp (Cyprinus carpio L.

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    Melika Ghelichpour

    2014-07-01

    Full Text Available Effects of ambient copper was investigated on serum stress markers, sodium and enzyme levels in Common carp (Cyprinus carpio L. over a 14-d exposure period. Fish were exposed to 0, 25 and 100 μg L-1 copper (as copper sulfate and blood was sampled at 0, 3, 7 and 14 d after exposure. Serum profile was significantly affected by copper concentration, sampling time and their interaction. Increase in serum levels of cortisol, glucose, alanine aminotransferase and aspartate aminotransferase and decrease in serum sodium levels were observed in both copper-exposed groups, 3 d after copper exposure, which lasted until the end of the experiment. It is concluded that copper exposure causes stress response and sodium loss in common carp. Likewise alanine aminotransferase and aspartate aminotransferase increase after exposure which might be as results of either tissue damage or stress.

  10. FACTORS AFFECTING TRANSMISSION AND SPREAD OF THE VIRUS SPRING VIREMIA OF CARP (CYPRINUS CARPIO

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    N. Kharkavlyuk

    2014-09-01

    Washington and Missouri states in 2004 and in Upper Mississippi in 2007. A SVC-like virus was found in cultivated shrimps in Hawaii. Natural outbreaks of a SVC infection were registered in following cyprinids: common carp (Cyprinus carpio carpio and koi carp (Cyprinus carpio koi, Crucian carp (Carassius carassius, silver carp (Hypophthalmichthys molitrix, bighead carp (Aristichthys nobilis, grass carp (Ctenopharyngodon idella, Prussian carp (Carassius auratus, ide (Leuciscus idus, tench (Tinca tinca and bream (Abramis brama. SVCV transmission is horizontal but vertical transmission from the ovarial liquid of carp has been established as well. Among virus transmission vectors are invertebrate parasites Argulus foliaceus (Crustacea, Branchiura and Piscicola geometra (Annelida, Hirudinea. There is also mechanical transmission of the virus with the aid of fishing gears. The virus can persist in water and silt for a long period of time. Originality. Measures for the prevention of infection of specialized fish farms have been proposed based on the performed analysis. Practical value. The analysis allows developing modern methods of diagnostics and control of the virus spread.

  11. Biochemical changes and sensory assessment on tissues of carp (Cyprinus carpio, Linnaeus 1758) during sale conditions.

    Science.gov (United States)

    Duran, Ayhan; Talas, Zeliha Selamoglu

    2009-11-01

    In this study, some biochemical changes of carp (Cyprinus carpio, Linnaeus 1758) tissues were investigated. Studies have been carried out on carp which have regional economical importance. Storage temperature and time are the most important factors that affect the quality of fish during sales. It was observed that the temperature varied between 9 and 12 degrees C in sale conditions. In addition, we assumed the arrival time of the fish at the fish market to be 0 (zero) h. Biochemical analyses [malondialdehyde (MDA) levels and catalase activity] of carp tissues (muscle, liver, heart, spleen, brain) were carried out on fish which were held for 24 and 48 h, as well as on fresh fish (0 h). In addition, sensory analysis was conducted by a panel consisting of experienced judges of sensory evaluation. Statistically significant (P 0.05) increase in MDA level in heart tissue of carp after 24 h. There was a statistically significant (P deals with the effects of post-slaughter time and storage temperature on carp tissues. It is concluded that by considering the storage temperature (9-12 degrees C) and storage time (post-slaughter) the product maintained acceptable quality up to 24 h. There was significant deterioration of sensory quality, as a result of changes in chemical constituents. PMID:19533401

  12. A review of introduction of common carp Cyprinus carpio in Pakistan: origin, purpose, impact and management

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    Khan Muhammd Naeem

    2016-06-01

    Full Text Available Common carp Cyprinus carpio was introduced from Thailand to Pakistan in 1964 for the purpose of aquaculture. Due to its high tolerance to temperature and turbidity, and prolific pond breeding habit, it was established promptly in most of natural inland waters, including rivers, lakes, streams, canals, wetlands and even village ponds of the country. Although common carp became one of the most abundant cyprinid species in inland waters and important food fish in Pakistan, its impact is not well documented. Fish farming of common carp has been carried out in Pakistan since 1970; initially it grew slowly but now it is playing an important role in the economy of the country by employing more than 400,000 people. Nowadays, farming of freshwater carps is present throughout Pakistan, especially in the provinces of Punjab and Sindh. There is a huge potential in common carp farming and it could help increase the livelihood of people and gross domestic product (GDP of the country as well. Still, there is a need to improve the fish farming practice to meet the world-class demands that could only be possible by the keen interest of policy makers and stake holders with better management.

  13. Benzocaine hydrochloride anesthesia in carp (Cyprinus carpio / Cloridrato de benzocaína na anestesia de carpas (Cyprinus carpio

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    Marco Antonio da Rocha

    2008-08-01

    Full Text Available Fish anesthesia is indicated to allow the accomplishment of several procedures such as biometry, tagging, transportation, physical examination, surgical procedures, and reproductive management. The doses of benzocaine in the carp anesthesia (Cyprinus carpio were determined, carrying through six phases with 40 fish each. The average weight of carps in each phase was of 147.45±7.99g, 173.32±9.15g, 191.26±14.05g, 269.84±19.24g, 285.25±17.97g, and 300.91±16.45g. In each phase, fish had been captured and placed in four containers each one with different concentrations of benzocaine (100, 140, 180 and 220 mg/L respectively. The induction time (IT was registered for each fish and after that the anesthetic induction biometry was performed. In each phase the minimal dose of benzocaine was calculated using the Linear Response Plateau (LRP, in a model that included dose and IT. The LRP was calculated for each phase: 125.79mg/L in 114.33s, 155.68mg/L in 115.75s, 145.33mg/L in 102.52s, 149.50mg/L in 140.53s, 166.42mg/L in 116.15s, and 158.34mg/L in 102.00s. The optimal dose was related with the weight, resulting in the equation: dose=114.230+0.158 x weight (r2=0.53. The equation shows that an increase in the weight in 1g corresponds to an increase of 0.158 mg/L in the dose of benzocaine hydrochloride for carps.A anestesia em peixes é indicada para permitir a realização de diversos procedimentos como: biometria, marcação, transporte, exame físico, procedimentos cirúrgicos e manejo reprodutivo. Determinou-se a dose de benzocaína na anestesia de carpas (Cyprinus carpio, com a realização de seis etapas com 40 peixes cada. O peso médio das carpas em cada etapa foi de 147,45±7,99g, 173,32±9,15g, 191,26±14,05g, 269,84±19,24g, 285,25±17,97g, e 300,91±16,45g. Em cada etapa, os peixes foram capturados e colocados em quatro recipientes com benzocaína nas concentrações de 100, 140, 180 e 220 mg/L respectivamente. O tempo de indução (TI foi

  14. Genetic improvement of common carp (Cyprinus carpio L.)

    OpenAIRE

    Vandeputte, Marc

    2009-01-01

    Common carp was the first domesticated fish species and is now a major species in worldwide aquaculture. Although research has generated a considerable body of knowledge about its genetics, genetic improvement programmes are virtually non-existent in this species and only crossbreeding is used in practice. The main reasons are the inefficiency of methods usually effective in other aquaculture species (monosexing, triploidy), and the bad image of selective breeding which is difficult to implem...

  15. The Utilization of Carp (Cyprinus carpio) Flesh as Fish Ball

    OpenAIRE

    YANAR, Yasemen

    1999-01-01

    In this study, the sensory properties and shelf life of fish balls prepared from carp flesh were investigated. Four study groups were prepared with different combinations of ingredients: garlic, garlic-sunflower oil, onion and onion-sunflower oil. A control group was also prepared. At the end of the sensory evaluation, the groups received between 7.4 and 9.07 points from the panelists regarding appearance, texture, succulence, odour and flavour and general acceptance. The onion and garlic ...

  16. Chlorinated paraffins: disposition of a polychloro-[l-14C]hexadecane in carp (Cyprinus carpio) and bleak (Alburnus alburnus)

    International Nuclear Information System (INIS)

    The disposition of polychloro-[l-14C]hexadecane was studied in carp (Cyprinus carpio) and bleak (Alburnus alburnus). After intra-arterial injection in carp about 6% of dose was excreted as 14CO2 in 96 h. The autoradiographic distribution pattern after administration of PCHD to carp and bleak was characterized by a strong labelling in the bile/intestinal contents. High radioactivity also appeared in the kidney, liver, gills and, particularly in bleak, in the nasal cavity, lens and skin. (Auth.)

  17. Bioaccumulation of heavy metals in organs of fresh water fish Cyprinus carpio (Common carp)

    International Nuclear Information System (INIS)

    The objective of the present study is to determine the bioaccumulation of heavy metals in various organs of the fresh water fish exposed to heavy metal contaminated water system. The experimental fish was exposed to Cr. Ni, Cd and Pb at sublethal concentrations for periods of 32 days. The elements Cd, Pb, Ni and Cr were assayed using Shimadzu AA 6200 atomic absorption spectrophotometry and the results were given as μg/g dry wt. The accumulation of heavy metal gradually increases in liver during the heavy metal exposure period. All the results were statistically significant at p Pb > Ni > Cr and Pb > Cd > Ni > Cr. Similarly, in case of kidney and flesh tissues, the order was Pb > Cd > Cr > Ni and Pb > Cr > Cd > Ni. In all heavy metals, the bioaccumulation of lead and cadmium proportion was significantly increased in the tissues of Cyprinus carpio (Common carp)

  18. G-protein coupled estrogen receptor (GPER) inhibits final oocyte maturation in common carp, Cyprinus carpio.

    Science.gov (United States)

    Majumder, Suravi; Das, Sumana; Moulik, Sujata Roy; Mallick, Buddhadev; Pal, Puja; Mukherjee, Dilip

    2015-01-15

    GPR-30, now named as GPER (G protein-coupled estrogen receptor) was first identified as an orphan receptor and subsequently shown to be required for estrogen-mediated signaling in certain cancer cells. Later studies demonstrated that GPER has the characteristics of a high affinity estrogen membrane receptor on Atlantic croaker and zebra fish oocytes and mediates estrogen inhibition of oocyte maturation in these two distantly related teleost. To determine the broad application of these findings to other teleost, expression of GPER mRNA and its involvement in 17β-estradiol mediated inhibition of oocyte maturation in other cyprinid, Cyprinus carpio was investigated. Carp oocytes at pre-vitellogenic, late-vitellogenic and post-vitellogenic stages of development contained GPER mRNA and its transcribed protein with a maximum at late-vitellogenic oocytes. Ovarian follicular cells did not express GPER mRNA. Carp oocytes GPER mRNA was essentially identical to that found in other perciformes and cyprinid fish oocytes. Both spontaneous and 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P)-induced oocyte maturation in carp was significantly decreased when they were incubated with either E2, or GPER agonist G-1. On the other hand spontaneous oocyte maturation was significantly increased when carp ovarian follicles were incubated with an aromatase inhibitor, fadrozole, GPER antagonist, G-15 and enzymatic removal of the ovarian follicle cell layers. This increase in oocyte maturation was partially reversed by co-treatment with E2. Consistent with previous findings with human and fish GPR30, E2 treatment in carp oocytes caused increase in cAMP production and simultaneously decrease in oocyte maturation, which was inhibited by the addition of 17,20β-P. The results suggest that E2 and GPER play a critical role in regulating re-entry in to meiotic cell cycle in carp oocytes. PMID:25485460

  19. COMPARATIVE ANALYSIS OF PLASTIC ATTRIBUTES OF DIFFERENT CARP KOI FORMS (CYPRINUS CARPIO KOI

    Directory of Open Access Journals (Sweden)

    Lysak O. O.

    2014-08-01

    Full Text Available Japanese carp or carp koi ( Cyprinus carpio koi – is a freshwater, partially saltwater fish which is a decorative subspecies of carp ( Cyprinus carpio L. namely a primary black form of carp koi that has its domestic name Magoi. Constant complex activity of abiotic and biotic factors together with husbandry and selectional activity of human caused significant changes in water ecosystems that impacted on the biology of carp koi subspecies (Cyprinus carpio koi and as a result caused the species-specific morphological adaptations in fish. Collection of materials was conducted from ornamental water ponds at the territory of Nemishaievo state agrotechnical college in the spring 2012. Author selected 3000 units of this year fish offsprings of carp koi with different colors. All the studied fish was divided into groups according to external indexes ( among them color in the following way: group I (sample – grey and white form (Hikarimuji which is the most similar to Magoi, group II – black and white form (Kumonryu, group III – black and yellow form ( Utsurimono, group IV – red and white form (Kohaku. For further researches scientists selected 25 exemplars of carp koi for each group. It is necessary to have a starting point (sample for statistical research of differences in carp koi forms. Sample or optimal form was determined by distances of deviations of scalar sums of form vectors of carp koi from nyvkivskyi carp. Morphometric study of carp koi was conducted according to 30 plastic attributes, in accordance with method proposed by I. F. Pravdin. In contrast with generally accepted relative magnitudes of indexes of plastic attributes of carp koi body were calculated from zoological (not standard length of the body of the fish that is connected with using koi as an object of decorative aquaculture. Statistic processing was conducted with using of average arithmetical discrepancy, root-mean-square error and variability index. Actuality of

  20. REARING CARP LARVAE (Cyprinus carpio IN CLOSED RECIRCULATORY SYSTEM (RAS

    Directory of Open Access Journals (Sweden)

    Anđelko Opačak

    2012-03-01

    Full Text Available Postembryonic rearing of carp larvae in closed recirculatory system was conducted in 2009 at the fish farm Ribnjak LLC, Donji Miholjac, Croatia. The research was conducted in two test groups (A and B with three iterations in each with a control group (C. Test group A (3 tanks x 250 l consisted of 150 000 larvae (density of 200 larvae•l-1, test group B (3 tanks x 500 l consisted of 600 000 larvae (density of 400 larvae•l-1, and the control group (C was a mud fish pond T-6 which was stocked by 800 000 larvae•ha-1 under standard production conditions. In this research, basic physical and chemical water parameters were controlled (temperature, oxygen, pH, total ammonia and nitrites. Initial measuring of carp larvae total length (TL was conducted prior to their placement into tanks (N=120. On the fourth, sixth, eighth and tenth day of research 20 larvae (N=140 were taken out of every tank as well as out of control group and measured. Feeding with live feed began on the third day after hatching (larval TL 6.00±0.36 mm. Ten minutes after feeding live feed to larvae for the first time, 20 larvae (N=120 were taken out of every tank and a high portion of larvae that accepted live feed (89.17±3.76% was determined by a magnifying glass. Feeding artificial feed began on the seventh day after the hatching. After ten minutes, a high portion of larvae who accepted artificial feed (96.67±2.58% was determined. Since the end of the research, the determined length increment (ITL per day was 0.41±0.04 mm, a very high survival rate was established (group A: 96%, group B: 93%. Feeding frequency was four times a day in five-hour intervals (at 06:00, 11:00,16:00 and 21:00 hours. The research was terminated after ten feeding days due to deteriorating condition of zoohygienic filter. The total of 3807 g of live feed and 1080 g of artificial feed was used.

  1. Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio

    Science.gov (United States)

    Cheng, Zhenyan; Li, Jinghui; Zhang, Baolong; Fang, Zhenzhen; Sun, Jinhui; Bai, Dongqing; Sun, Jinsheng; Qiao, Xiuting

    2016-06-01

    A 9-week feeding trial in floating freshwater cages (1.0 m×1.0 m×2.0 m) was conducted to study the effects of diff erent dietary levels of protein and starch on growth, body composition, and gene expression of enzymes in common carp, Cyprinus carpio (mean body weight, 36.12±1.18 g) to evaluate the protein-sparing effect of dietary carbohydrate. Four diets were formulated with corn starch as the carbohydrate source to obtain corn starch levels of 6.5%, 13%, 19.5%, or 26% and protein levels of 30.5%, 28.2%, 26.4%, and 24.2%. The results showed no diff erences in growth performance of fish fed the diets with diff erent protein and corn starch levels, but body composition and glucose metabolic enzyme activity of carp were significantly aff ected by the diff erent diets (Pphosphate (G6Pase) activity decreased with increasing dietary corn starch level (P0.05). In summary, the results indicate a protein-sparing effect by substituting carbohydrate in the diet of common carp.

  2. Effects of Dietary Exposure to Zearalenone (ZEN on Carp (Cyprinus carpio L.

    Directory of Open Access Journals (Sweden)

    Constanze Pietsch

    2015-08-01

    Full Text Available The mycotoxin zearalenone (ZEN is frequently contaminating animal feeds including feed used in aquaculture. In the present study, the effects of dietary exposure to ZEN on carp (Cyprinus carpio L. were investigated. ZEN at three different concentrations (low dose: 332 µg kg−1, medium dose: 621 µg kg−1 and high dose: 797 µg kg−1 final feed, respectively was administered to juvenile carp for four weeks. Additional groups received the mycotoxin for the same time period but were fed with the uncontaminated diet for two more weeks to examine the reversibility of the ZEN effects. No effects on growth were observed during the feeding trial, but effects on haematological parameters occurred. In addition, an influence on white blood cell counts was noted whereby granulocytes and monocytes were affected in fish treated with the medium and high dose ZEN diet. In muscle samples, marginal ZEN and α-zearalenol (α-ZEL concentrations were detected. Furthermore, the genotoxic potential of ZEN was confirmed by analysing formation of micronuclei in erythrocytes. In contrast to previous reports on other fish species, estrogenic effects measured as vitellogenin concentrations in serum samples were not increased by dietary exposure to ZEN. This is probably due to the fact that ZEN is rapidly metabolized in carp.

  3. Mercury concentrations in common carp (Cyprinus carpio) in Lake Chapala, Mexico: a lakewide survey.

    Science.gov (United States)

    Stong, Todd; Alvarado Osuna, Claudia; Shear, Harvey; de Anda Sanchez, José; Ramírez, Gerardo; Díaz Torres, José de Jesús

    2013-01-01

    Previous studies, based on limited data, found elevated levels of mercury in carp in Lake Chapala, Mexico. The extent of mercury contamination in carp throughout the Lake has not been determined. In order to obtain reliable information about total mercury concentration in carp (Cyprinus carpio), 262 fish from 27 sites (approximately 10 fish per site) throughout the lake were analyzed. Results were expressed as the mean and median of the results at each site. Only one of the samples exceeded Mexican National Standard (1.0 ppm) for mercury in fish flesh. We discuss these results in comparison to World Health Organization (WHO), US Food and Drug Administration (FDA) and US Environmental Protection Agency (US EPA) criteria; many of our samples exceed these criteria based on Tolerable Daily Intake (TDI) or Reference Dose (RfD). ANOVA of four groups of mercury results clustered by distance from the Lerma showed statistically significant differences (P = 0.0071) between the group closest to, versus farthest from, the Lerma River. PMID:24007438

  4. Common carp (Cyprinus carpio L.) alters its feeding niche in response to changing food resources: direct observations in simulated ponds

    OpenAIRE

    Rahman, M.M.; Kadowaki, S.; Balcombe, S.R.; Wahab, M.A.

    2010-01-01

    We used customized fish tanks as model fish ponds to observe grazing, swimming, and conspecific social behavior of common carp (Cyprinus carpio) under variable food-resource conditions to assess alterations in feeding niche. Different food and feeding situations were created by using only pond water or pond water plus pond bottom sediment or pond water plus pond bottom sediment and artificial feeding. All tanks were fertilized twice, prior to stocking and 2 weeks later after starting the expe...

  5. The seasonal changes in innate immunity of the common carp (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Buchtíková, S.; Šimková, A.; Rohlenová, K.; Flajshans, M.; Lojek, Antonín; Lilius, E. M.; Hyršl, P.

    2011-01-01

    Roč. 318, 1-2 (2011), s. 169-175. ISSN 0044-8486 Institutional support: RVO:68081707 Keywords : Cyprinus carpio * Respiratory burst * Phagocytes Subject RIV: BO - Biophysics Impact factor: 2.041, year: 2011

  6. Reliability of non-lethal assessment methods of body composition and energetic status exemplified by applications to eel (Anguilla anguilla) and carp (Cyprinus carpio)

    DEFF Research Database (Denmark)

    Klefoth, Thomas; Skov, Christian; Aarestrup, Kim; Arlinghaus, Robert

    2013-01-01

    (Anguilla anguilla) and carp (Cyprinus carpio) and the dorsal white muscle of carp. Homogenizedfish flesh was used for calibration. Relative dry mass was strongly correlated with relative fat content(R2up to 96.7%) and energy density (R2up to 99.1%). Thus, calibrations were only conducted for relativedry...

  7. Genetic potential analysis of German mirror carp (Cyprinus carpio L.) using microsatellite markers

    Institute of Scientific and Technical Information of China (English)

    Ning HOU; Xiaoshu HOU; Yong LI; Dayu LI; Fujun LIU; Ruixin MAO; Xiaowen SUN

    2008-01-01

    Using 30 microsatellite markers and combin-ing quantifiable characteristics such as body weight, body length and body width, we evaluated the genetic potential of 3 German mirror carp (Cyprinus carpio L.) popula-tions. Number of effective alleles (Ae), observed (Ho) and expected (He) heterozygosity values and polymorphic information contents (PIC) were all calculated. Two hun-dred and eighty-seven alleles and 559 genotypes were detected. The DNA fragment length was 109-400 bp. The Hardy-Weinberg Equilibrium was checked and the phenomenon of some disequilibrium was studied accord-ing to the χ2test. The results showed that the level of genetic variability was moderate, but genetic potential of Shuanglai population was much lower than that of Huanxin and Songpu breeding populations. PIC of the three populations of German mirror carp were between 0.08787 and 0.5377, both highly and moderately poly-morphic markers were 13. The number of the Ae was between 1.1014 and 6.4665. The Ho and He heterozygos-ity values were 0.0968-0.9892 and 0.0926-0.8554, respect-ively. The linkage correlation was analyzed using the data of body weight, body length and body width, and 30 loci. The result showed that there existed 2 loci, HLJ319 and HLJ693, associated with body length. The HLJ693 locus was significantly correlated with body weight trait. The HLJ677 locus was linked with body width. And then the result was verified in Recombinant Inbred Lines (RIL) of common carp. It showed that the HLJ319 locus was sig-nificantly linked with body length, the same as the result of quantitative trait loci (QTL) location for common carp.

  8. Only small fractions of soluble ß-glucan modulate the mucosal immune system in carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Przybylska, Dominika Alicja; Nielsen, Michael Engelbrecht

    understand the effect of β-glucan induced modulation in carp in relation to tissue regeneration, mucosal immunity and host-pathogen interactions. Expression profiles of immune related genes will be measured in fresh water specie – common carp (Cyprinus carpio L.). The methodology of the project involves the...... usage of real-time quantitative PCR to quantify expression of genes of interest (IL-1β, IL-10, TNF-α, IL-6). This study will provide further understanding on the effect of β-glucan as a modulator which could improve fish welfare and health as well as having an economic potential production for the...... aquaculture industry....

  9. Stock evaluation and development of a breeding program for common carp (Cyprinus carpio) in Karnataka, India: progress of a research project

    OpenAIRE

    Basavaraju, Y.; Penman, D.J.; Mair, G.C.

    2003-01-01

    Common carp (Cyprinus carpio) is the single most important species for aquaculture in the state of Karnataka, India, where it is generally grown in polyculture with Indian major carps. Precocious maturation and unwanted reproduction in the species have been identified as constraints to increase production in aquaculture and culture-based fisheries in Karnataka state. Stocks of C. carpio obtained from Hungary (Amur and P3), Indonesia (Rajdanu) and Vietnam (SV) are being assessed alongside two ...

  10. Selective breeding programme of common carp (Cyprinus carpio L. in Serbia: Preliminary results

    Directory of Open Access Journals (Sweden)

    Spasić Milan M.

    2010-01-01

    Full Text Available The aim of this study was to estimate heritability and genetic correlations between weight, length and height of common carp in Serbia (Cyprinus carpio L. during 3-year growth period. The 50 families of common carp were produced in 2007 and used for the estimation of genetic parameters. The fish were measured at tagging for weight, length and height (W0, L0, H0, then during the first autumn (W1, L1, H1 and during the second autumn (W2, L2, H2. Based on univariate models heritability estimates were high for all traits (0.39, 0.34 and 0.45 for W1, L1 and H1, respectively and also for the second production year (0.49, 0.47 and 0.44 for W2, L2 and H2, respectively. The genetic correlations were estimated using multivariate models and they were high between W1 and L1 and H1 (0.81 ± 0.06 and 0.91 ± 0.03 for L1 and H1, respectively, while between H1 and L1 were moderately correlated (0.54 ± 0.12. In the second production year genetic correlations were also high, between W2 and L2 and H2 (0.64 ± 0.09 and 0.74 ± 0.06, respectively, while between length and height they were lower (0.24 ± 0.15. Based on the current results improving growth rate of common carp through genetic selection is expected to be effective.

  11. Dispersion of the invasive common carp Cyprinus carpio in southern South America: changes and expectations, westward and southward.

    Science.gov (United States)

    Crichigno, S; Cordero, P; Blasetti, G; Cussac, V

    2016-07-01

    Common carp Cyprinus carpio possess multiple traits that contribute to their success as an invasive species. They have been introduced across the globe, and abundant populations can have numerous negative effects. Although ecological niche-based modelling techniques have been used to predict the potential range of C. carpio invasion in U.S.A., occurrence and abundance patterns have not yet been considered on a regional scale. In the present review new locations are documented, the status of the southernmost population has been studied and the probability of new lakes and reservoirs being colonized by C. carpio has been obtained and related to environmental conditions. The new localities for C. carpio have expanded its distribution westward, into the Andean Region, and present results from the South American southernmost population have shown a well-established population. Analysis of presence data provided two principal results: (1) the probability of a site being with C. carpio can be inferred using environmental variables and (2) the probability of a site being with C. carpio is a useful tool for the prediction of future invasions. Selective fishing on the Negro basin could constitute a potential mitigation measure, decreasing the abundance of the species and thus reducing the species' potential for southward expansion. These results reinforce the idea that artisanal fisheries, food production and conservation interests should be taken into account by local government management agencies in any discussion regarding the southern distribution of C. carpio in the near future. PMID:27095064

  12. Gut microbiota contributes to the growth of fast-growing transgenic common carp (Cyprinus carpio L..

    Directory of Open Access Journals (Sweden)

    Xuemei Li

    Full Text Available Gut microbiota has shown tight and coordinated connection with various functions of its host such as metabolism, immunity, energy utilization, and health maintenance. To gain insight into whether gut microbes affect the metabolism of fish, we employed fast-growing transgenic common carp (Cyprinus carpio L. to study the connections between its large body feature and gut microbes. Metagenome-based fingerprinting and high-throughput sequencing on bacterial 16S rRNA genes indicated that fish gut was dominated by Proteobacteria, Fusobacteria, Bacteroidetes and Firmicutes, which displayed significant differences between transgenic fish and wild-type controls. Analyses to study the association of gut microbes with the fish metabolism discovered three major phyla having significant relationships with the host metabolic factors. Biochemical and histological analyses indicated transgenic fish had increased carbohydrate but decreased lipid metabolisms. Additionally, transgenic fish has a significantly lower Bacteroidetes:Firmicutes ratio than that of wild-type controls, which is similar to mammals between obese and lean individuals. These findings suggest that gut microbiotas are associated with the growth of fast growing transgenic fish, and the relative abundance of Firmicutes over Bacteroidetes could be one of the factors contributing to its fast growth. Since the large body size of transgenic fish displays a proportional body growth, which is unlike obesity in human, the results together with the findings from others also suggest that the link between obesity and gut microbiota is likely more complex than a simple Bacteroidetes:Firmicutes ratio change.

  13. Identification of furan fatty acids in the lipids of common carp (Cyprinus carpio L.).

    Science.gov (United States)

    Chvalová, Daniela; Špička, Jiří

    2016-06-01

    Fatty acid (FA) composition was analyzed in muscle and gonad tissues of marketed common carp (Cyprinus carpio). The extracted lipids were separated into four fractions: polar lipids (PL), diacylglycerols, free fatty acids and triacylglycerols (TAG) using thin layer chromatography. FA content within the lipid fractions was determined by gas chromatography with flame ionization detector (GC/FID). The muscle lipids consisted primarily of TAG (96.9% of total FA), while PL were the major component of both male (67.6%) and female gonad (58.6%) lipids. Polyunsaturated fatty acids predominated in PL of all tissues (52.2-55.8% of total FA); monounsaturated fatty acids were the most abundant FA group in TAG of muscle (51.8%) and female gonads (47.8%) whereas high proportion of furan fatty acids (F-acids) (38.2%) was detected in TAG of male gonads. Eight F-acids were identified by gas chromatography-mass spectrometry (GC/MS) in male gonad samples, including less common 12,15-epoxy-13,14-dimethylnonadeca-12,14-dienoic acid with even-numbered alkyl moiety. PMID:26830577

  14. Kinetics of cadmium accumulation and elimination in carp Cyprinus carpio tissues

    International Nuclear Information System (INIS)

    Carp (Cyprinus carpio) were tested for cadmium accumulation and elimination during and after a simulated pollution exposure. Fish were distributed in two 1000-l indoor concrete aquaria supplied with a continuous flow (8 l min-1) of well water. The cadmium concentration was maintained at 53 μg l-1 in one aquarium and 443 μg l-1 in the other aquarium for 127 days. The exposure phase was followed by a 43-day depuration period. The cadmium accumulation in liver, kidney and muscle was measured by means of ICP-MS. The data showed that cadmium exposure produces significant cadmium uptake in tissues. Cadmium concentrations increased sharply in kidney and liver, whereas the pollutant level in muscle was only significant after 106 days. After 127 days of Cd exposure (53 μg l-1), the cadmium concentration in kidney was 4-fold higher than in liver and 50-fold higher than in muscle for a toxic level of 53 μg l-1. At a Cd of 443 μg l-1, kidney cadmium content was 2-fold higher than in liver and 100-fold higher than in muscle. In kidney and liver, the toxic concentration increased as the concentration of pollutant in water increased. During the 43 depuration days, the loss of accumulated cadmium was rapid and immediate in muscle. Conversely, no loss of cadmium was observed in kidney and liver. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

  15. Toxicity of Citrate-Capped Silver Nanoparticles in Common Carp (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Byoungcheun Lee

    2012-01-01

    Full Text Available Juvenile common carp (Cyprinus carpio were used as a model to investigate acute toxicity and oxidative stress caused by silver nanoparticles (Ag-NPs. The fish were exposed to different concentrations of Ag-NPs for 48 h and 96 h. After exposure, antioxidant enzyme levels were measured, including glutathione-S-transferase (GST, superoxidase dismutase, and catalase (CAT. Other biochemical parameters and histological abnormalities in different tissues (i.e., the liver, gills, and brain were also examined. The results showed that Ag-NPs agglomerated in freshwater used during the exposure experiments, with particle size remaining <100 nm. Ag-NPs had no lethal effect on fish after 4 days of exposure. Biochemical analysis showed that enzymatic activities in the brain of the fish exposed to 200 g/L of Ag-NPs were significantly reduced. Varied antioxidant enzyme activity was recorded in the liver and gills. Varied antioxidant enzyme activity was recorded for CAT in the liver and GST in the gills of the fish. However, the recovery rate of fish exposed to 200 g/L of Ag-NPs was slower than when lower particle concentrations were used. Other biochemical indices showed no significant difference, except for NH3 and blood urea nitrogen concentrations in fish exposed to 50 g/L of Ag-NPs. This study provides new evidence about the effects of nanoparticles on aquatic organisms.

  16. Protein sources and their significance in carp (Cyprinus carpio L. nutrition

    Directory of Open Access Journals (Sweden)

    Stanković Marko B.

    2011-01-01

    Full Text Available Common carp (Cyprinus carpio is one of the most widely cultured fish species in the world. It is predominantly cultivated in the Balkans in the semiintensive system that is based on the utilization of natural food from the fish pond and supplemental feed (cereals, pelleted and extruded feed. Currently, an intensified type of semi-intensive production is starting to be more present in Serbia, where cereals, as the most common supplemental feed, are replaced by concentrated feed that fill in the lack of proteins from carps’ natural food in periods of its decreased production. The nutritional requirements for growth, reproduction and normal physiological functions of fish are similar to other animals, but generally fish need more proteins in their diet. Due to this, the efficiency in the use and utilization of proteins is more significant for fish than for other animals. The selection of supplemental feed in semi-intensive system of fish culture depends on the natural potential of the fish pond, culturing period, fish category, price and quality of feed.

  17. 蓝色鳞(Cyprinus carpio blue vat)的细胞遗传学分析%Cytogenetic Analysis of Blue Common Carp Cyprinus carpio blue var

    Institute of Scientific and Technical Information of China (English)

    薛淑群; 徐伟

    2011-01-01

    The diploid chromosome number, karyotype, and DNA content were studied in metaphase chromosome in kidney cells and blood of blue common carp Cyprinus carpio blue var injected with PHA and colchicin by airdrying technique. It was found that the diploid chromosome number was 2n=100. The karyotype was found to be composed of 30 m, 26sm and 44st+t chromosomes, and NF=156. The diploid nucleus DNA content was measured from the somatic cell of the blue common carp using flow cytometer (Beckman cellLab Quant, made in USA) and the DNA content (2.3 pg· N^+) in erythrocytes of chick (Gallus sp) as standard. The common carp had DNA content of3.99pg·N^-1, corresponding to the result measured chromosome number as ploidy.%采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备蓝色鳞鲤染色体,对100个中期分裂相记数统计,确定蓝色鳞鲤(Cyprinus carpio blue var)的染色体数为2n=100。测得核型参数按Levan等的染色体划分标准得出:蓝色鳞鲤有15对中部着丝点染色体(m);13对亚中部着丝点染色体(sin);22对端部和亚端部着丝点染色体(st,t),其染色体总臂数(NF)为156,蓝色鳞鲤核型公式为2n=30m+26sm+44st,t。采用流式细胞分析仪测定了蓝色鳞鲤的DNA含量,与鸡血细

  18. EVALUATION OF ETHINYLESTRADIOL (EE2 EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO

    Directory of Open Access Journals (Sweden)

    GABI DUMITRESCU

    2013-07-01

    Full Text Available Worldwide, the scientific researches performed during the last years are focused on the determination of the negative effects caused by natural and antropogeneous chemical compounds on aquatic species; these species are more exposed to most pollutants than the land species, for the simple reason that the aquatic environment is the last destination for most residues. Our research team proposed to test the toxic effect caused by ethinylestradiol on embryo development in common carp (Cyprinus carpio. Common carp embryos were purchased from the fish farm S.C. Acva Prod S.R.L. Cefa, Bihor County these were obtained by artificial reproduction. After taking and selection, the fecundated spawns were introduced in 10 Nunk culture plates of 45 ml, where we introduced 40 ml water, too. We created 3 batches, with two replications, namely: batch 1 – control, batch 2 – in water, we added ethinylestradiol (EE2 in concentration of 1.5 ng L-1 and batch 3 – we added in water a concentration of 7 ng L-1 EE2. During the incubation, the Nunk plates were kept in breeding aquariums, at a temperature of 24°C. Successive to the supervision of embryos in batch 3, 48 hours post-fecundation, we could observe evolution stagnations, 70% of them being in the stage of 40 somites of the segmentation period. At the same age, 100% of the control batch- embryos entered the stage of advanced faringula, and in batch 2 all embryos were in the stage of incipient faringula. 60-72 hours post-fecundation, all embryos in the batch 3 died, 90% in the 40 somite stage of the segmentation period and 10% in the stage of incipient faringula. 85 hours post-fecundation, all embryos belonging to the control batch were in the larva stage, while in batch 2, 90% were in the larva stage and 10% died in the stage of advanced faringula.

  19. The effect of extenders, cryoprotectants and cryopreservation methods on common carp (Cyprinus carpio) sperm.

    Science.gov (United States)

    Irawan, Henky; Vuthiphandchai, Verapong; Nimrat, Subuntith

    2010-12-01

    The objective of this study was to determine the effects of various extenders, cryoprotectants and cryopreservation methods on post-thaw sperm motility and duration of sperm motility in common carp (Cyprinus carpio). We focused on freezing of common carp sperm utilizing a practical and inexpensive protocol for aquaculture. Sperm were diluted 1:1 in one of six extenders (common carp sperm extenders; CCSE 1-CCSE 6) containing three types of cryoprotectants (dimethyl sulfoxide; DMSO, methanol; MET and propylene glycol; PG) at a final concentration of 10%, and frozen at a rate of 10°C/min from an initial temperature 25 to -40°C before storage in liquid nitrogen. The results demonstrated that sperm diluted with CCSE 2 and DMSO had the best post-thaw motility (94.5 ± 3.3%), similar to that of the control (98.6 ± 0.7%; P>0.05). Duration of sperm motility from a treatment with CCSE 2 and DMSO (97 ± 20.8s) was not significantly different (P>0.05) from that of the control (73.3 ± 12.9s). A second experiment studied the effects of various cryopreservation methods on post-thaw sperm motility and duration of sperm motility, based on using CCSE 2 and DMSO in all treatments. Sperm were frozen using different cryopreservation methods: direct immersion into liquid nitrogen, controlled-rate programmable freezer, or exposure to liquid nitrogen vapor at different heights and time. Sperm frozen at a height of 2 cm above liquid nirogen surface for 10 min gave the highest post-thaw sperm motility (91.7 ± 7.8%) and longest duration of post-thaw sperm motility (105.7 ± 23.1s). Sperm frozen 2 cm above liquid nitrogen surface for 10 min produced the highest fertilization and hatching rate of about 73.6 ± 6.5% and 62.8 ± 5.9%, respectively, not significant different (P>0.05) from those of fresh sperm (75.6 ± 7.5% and 66.5 ± 4.8%, respectively). This study reports superior performance of the combination of CCSE 2 and DMSO for freezing common carp sperm that resulted in high

  20. Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L. Liver

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    Elenka Georgieva

    2013-12-01

    Full Text Available We aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG” on glycogen storage and expression of lipid droplets in common carp (Cyprinus carpio, L. liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory conditions. We used PAS-reaction for detection of glycogen storage and Sudan III staining for detection of lipid droplets in common carp hepatocytes. Hence, we found that the amount of glycogen and the fat storage in the liver increased proportionally with the increased fungicide concentrations. We also found conglomerates of accumulated glycogen in certain hepatocytes at all used concentrations. Overall, the results demonstrated enhanced glyconeogenesis and fat accumulation in the common carp liver, exposed to the test chemical.

  1. RESEARCH ABOUT THE INFLUENCE OF ORGANIC SELENIUM ON THE GROWTH PERFORMANCES OF THE COMMON CARP (CYPRINUS CARPIO, LAUSITZ VARIETY, JUVENILES

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    ALINA RODICA ANI

    2013-07-01

    Full Text Available The present research concerns the use of organic selenium (Se in the nutrition of the common carp (Cyprinus carpio, variety Lausitz, during its sapling stage. The experimental work was carried out during 159 days in the Martinesti Fish Farm, near the city of Cluj-Napoca, Romania. The total number of carp juveniles was organized in two batches: the experimental batch and the witness batch. Each group consists of 200 juveniles having a mean individual weight of 1.5 g. The rearing conditions were similar for the both groups, excepting the alimentation. The experimental batch received 0.03 mg organic Se (Sel-plex per kg of food. At the end of the experiment these juveniles reached an average weight of 354.619±0,088 g/specimen and a rate of survival of 99%, while the juveniles from the witness batch registered an average weight of 276.804±0,051 g/specimen and a rate of survival of 97%. The results reveal that the use of organic Se (Sel-plex in the nutrition of the common carp juveniles (Cyprinus carpio, variety Lausitz, leads to an increased body mass and to a lower mortality.

  2. Effect of Delayed Icing on Biogenic Amines Formation and Bacterial Contribution of Iced Common Carp (Cyprinus carpio)

    OpenAIRE

    Seyed Vali Hosseini; Ali Hamzeh; Mehran Moslemi; Aria Babakhani Lashkan; Antonio Iglesias; Xesús Feás

    2013-01-01

    The variation of six biogenic amines (BAs) and total viable count (TVC) in common carp (Cyprinus carpio) stored in ice with 0, 4 and 8 h delay before icing was evaluated in a period of 4 days. Delayed icing led to significant (p < 0.05) increases in TVC throughout the period of storage and showed a good correlation with BAs content. The obtained data showed that putrescine and cadaverine were predominant in all samples and it was indicated that they could be proper indicators to determine ...

  3. Swimming, grazing and social behaviour of rohu Labeo rohita (Hamilton) and common carp Cyprinus carpio (L.) in tanks under fed and non-fed conditions

    NARCIS (Netherlands)

    Rahman, M.M.; Verdegem, M.C.J.; Nagelkerke, L.A.J.; Wahab, M.A.; Verreth, J.A.J.

    2008-01-01

    Fish behaviour was monitored in 1-m2 tanks each stocked with three 67-g rohu (Labeo rohita). In addition, 80-g common carp (Cyprinus carpio) were stocked at 0, 1 or 2 fish per tank. All tanks were fertilized prior to stocking to stimulate natural food production. In addition, half of the tanks were

  4. Effects of day and night on swimming, grazing and social behaviours of rohu Labeo rohita (Hamilton) and common carp Cyprinus carpio (L.) in simulated ponds.

    NARCIS (Netherlands)

    Rahman, M.M.; Verdegem, M.C.J.; Wahab, M.A.; Hossain, M.Y.; Jo, Q.

    2008-01-01

    Diel rhythmicity of grazing, swimming, resting and social interactions of rohu (Labeo rohita) (weight 66.5¿68.3 g) and common carp (Cyprinus carpio) (79.9¿82.0 g) were observed in 1 m2 simulated ponds using video images. Fish behaviour was monitored during a full 24-h period, starting at 08:00 hours

  5. Genomic insight into the common carp (Cyprinus carpio genome by sequencing analysis of BAC-end sequences

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    Wang Jintu

    2011-04-01

    Full Text Available Abstract Background Common carp is one of the most important aquaculture teleost fish in the world. Common carp and other closely related Cyprinidae species provide over 30% aquaculture production in the world. However, common carp genomic resources are still relatively underdeveloped. BAC end sequences (BES are important resources for genome research on BAC-anchored genetic marker development, linkage map and physical map integration, and whole genome sequence assembling and scaffolding. Result To develop such valuable resources in common carp (Cyprinus carpio, a total of 40,224 BAC clones were sequenced on both ends, generating 65,720 clean BES with an average read length of 647 bp after sequence processing, representing 42,522,168 bp or 2.5% of common carp genome. The first survey of common carp genome was conducted with various bioinformatics tools. The common carp genome contains over 17.3% of repetitive elements with GC content of 36.8% and 518 transposon ORFs. To identify and develop BAC-anchored microsatellite markers, a total of 13,581 microsatellites were detected from 10,355 BES. The coding region of 7,127 genes were recognized from 9,443 BES on 7,453 BACs, with 1,990 BACs have genes on both ends. To evaluate the similarity to the genome of closely related zebrafish, BES of common carp were aligned against zebrafish genome. A total of 39,335 BES of common carp have conserved homologs on zebrafish genome which demonstrated the high similarity between zebrafish and common carp genomes, indicating the feasibility of comparative mapping between zebrafish and common carp once we have physical map of common carp. Conclusion BAC end sequences are great resources for the first genome wide survey of common carp. The repetitive DNA was estimated to be approximate 28% of common carp genome, indicating the higher complexity of the genome. Comparative analysis had mapped around 40,000 BES to zebrafish genome and established over 3

  6. Assimilation efficiencies of Cd and Zn in the common carp (Cyprinus carpio): Effects of metal concentration, temperature and prey type

    International Nuclear Information System (INIS)

    The impact of several factors on the assimilation efficiency (AE) of Cd and Zn from food in the common carp (Cyprinus carpio) was studied. Tested prey species were midge larvae (Chironomus riparius), zebra mussels (Dreissena polymorpha) and oligochaetes (Tubifex tubifex). The Cd load of the larvae did not affect the Cd AE in the carp. The Zn AE however, was negatively related to the Zn load of the prey. Food quantity and starvation of the carp did not significantly affect the Cd AE. For Zn, a significant decrease in AE was found when carp were fed ad libitum. Decreasing the temperature from 25 oC to 15 oC did not influence the Cd AE, while for Zn a significant decrease of the AE was measured. Carp assimilated Cd from both zebra mussels and oligochaetes with a significantly lower efficiency in comparison to the midge larvae, although Zn AEs was prey independent. - Assimilation efficiency of Cd and Zn in food of carp is affected by metal load, prey type and temperature

  7. Assimilation efficiencies of Cd and Zn in the common carp (Cyprinus carpio): Effects of metal concentration, temperature and prey type

    Energy Technology Data Exchange (ETDEWEB)

    Campenhout, K. van [Ecophysiology, Biochemistry and Toxicology Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium); Bervoets, L. [Ecophysiology, Biochemistry and Toxicology Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium)]. E-mail: lieven.bervoets@ua.ac.be; Blust, R. [Ecophysiology, Biochemistry and Toxicology Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp (Belgium)

    2007-02-15

    The impact of several factors on the assimilation efficiency (AE) of Cd and Zn from food in the common carp (Cyprinus carpio) was studied. Tested prey species were midge larvae (Chironomus riparius), zebra mussels (Dreissena polymorpha) and oligochaetes (Tubifex tubifex). The Cd load of the larvae did not affect the Cd AE in the carp. The Zn AE however, was negatively related to the Zn load of the prey. Food quantity and starvation of the carp did not significantly affect the Cd AE. For Zn, a significant decrease in AE was found when carp were fed ad libitum. Decreasing the temperature from 25 {sup o}C to 15 {sup o}C did not influence the Cd AE, while for Zn a significant decrease of the AE was measured. Carp assimilated Cd from both zebra mussels and oligochaetes with a significantly lower efficiency in comparison to the midge larvae, although Zn AEs was prey independent. - Assimilation efficiency of Cd and Zn in food of carp is affected by metal load, prey type and temperature.

  8. Organophosphate and carbamate insecticides in agricultural waters and cholinesterase (ChE) inhibition in common carp (Cyprinus carpio)

    Science.gov (United States)

    Gruber, S.J.; Munn, M.D.

    1998-01-01

    Cholinesterase (ChE) activity was used as a biomarker for assessing exposure of common carp (Cyprinus carpio) to organophosphate and carbamate insecticides from irrigated agricultural waters. Carp were collected from a lake (Royal Lake) that receives most of its water from irrigation return flows and from a reference lake (Billy Clapp Lake) outside of the irrigation system. Results indicated that the mean whole-brain ChE activity of carp from Royal Lake (3.47 μmol/min/g tissue) was 34.2% less than that of carp from Billy Clapp Lake (5.27 μmol/min/g tissue) (p = 0.003). The depressed ChE activity in brain tissue of Royal Lake carp was in response to ChE-inhibiting insecticides detected in water samples in the weeks prior to tissue sampling; the most frequently detected insecticides included chlorpyrifos, azinphos-methyl, carbaryl, and ethoprop. Neither sex nor size appears to be a covariable in the analysis; ChE activity was not correlated with fish length or weight in either lake and there was no significant difference in ChE activity between the two sexes within each lake. Although organophosphate and carbamate insecticides can break down rapidly in the environment, this study suggests that in agricultural regions where insecticides are applied for extended periods of the year, nontarget aquatic biota may be exposed to high levels of ChE-inhibiting insecticides for a period of several months.

  9. EVALUATION OF ETHINYLESTRADIOL (EE2) EFFECT ON EMBRYO DEVELOPMENT IN COMMON CARP (CYPRINUS CARPIO)

    OpenAIRE

    GABI DUMITRESCU; LILIANA PETCULESCU-CIOCHINA; VOIA S.; A. GROZEA; D. DRONCA; IULIANA CRETESCU; LILIANA BOCA

    2013-01-01

    Worldwide, the scientific researches performed during the last years are focused on the determination of the negative effects caused by natural and antropogeneous chemical compounds on aquatic species; these species are more exposed to most pollutants than the land species, for the simple reason that the aquatic environment is the last destination for most residues. Our research team proposed to test the toxic effect caused by ethinylestradiol on embryo development in common carp (Cyprinus ca...

  10. Metals Analysis in common carp (Cyprinus carpio from Shirinsu Wetland, Hamedan province, Iran

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    Soheil Sobhan Ardakani

    2015-10-01

    Full Text Available Background & Aims of the Study: Concentrations of Cd, Hg and As were measured by atomic absorption spectrometry (AAS in muscle, liver and gill tissues of common carp from the Shirinsu Wetland during February to March 2013. Methods: Fish samples (Cyprinus carpio were caught from Shirinsu Wetland, western Iran from February to March 2013. Specimens were frozen in prewashed polyethylene bags and frozen samples brought to the laboratory in ice chests. Samples (2 g were digested with 5 ml of HNO 3 (65% v/v, 1 ml of H 2 O 2 (30% v/v with a microwave oven. A blank digest was carried out in the same way. The digestion program began at a potency of 1200W then ramped for 10 min, after which samples were held for 10 min at 1200W. The second step began at a potency of 0W and held for 15 min. All metal concentrations were determined on a wet weight basis as μg g −1 . Results: Metal levels measured in muscle tissue were in the following ranges (μg g −1 : Cd 0.007-0.011, Hg 0.006-0.01 and As not detected. In liver tissue were (μg g −1 : Cd 0.035-0.043, Hg and As not detected. In gill tissue were (μg g −1 : Cd 0.31-0.55, Hg 0.002-0.004 and As 0.001-0.003. The results presented on metal contents in the examined tissues give an indication of the environmental conditions. Concentrations of Cd, Hg and As obtained were far below the established values by the European Community Regulations. However, Cd level found in gill tissue was higher than the recommended legal limits for human consumption and as such may cause a human health issue. Conclusion: Analytical data shows that the metal concentrations for the fish tissues were generally within the FAO/WHO, ASTDR and EEC recommended limits for fish. Therefore there is no serious health risk associated with the consumption of the three studied metals in the muscle and liver tissues analyzed.             

  11. Are fish immune systems really affected by parasites? an immunoecological study of common carp (Cyprinus carpio

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    Flajšhans Martin

    2011-06-01

    Full Text Available Abstract Background The basic function of the immune system is to protect an organism against infection in order to minimize the fitness costs of being infected. According to life-history theory, energy resources are in a trade-off between the costly demands of immunity and other physiological demands. Concerning fish, both physiology and immunity are influenced by seasonal changes (i.e. temporal variation associated to the changes of abiotic factors (such as primarily water temperature and interactions with pathogens and parasites. In this study, we investigated the potential associations between the physiology and immunocompetence of common carp (Cyprinus carpio collected during five different periods of a given year. Our sampling included the periods with temporal variability and thus, it presented a different level in exposure to parasites. We analyzed which of two factors, seasonality or parasitism, had the strongest impact on changes in fish physiology and immunity. Results We found that seasonal changes play a key role in affecting the analyzed measurements of physiology, immunity and parasitism. The correlation analysis revealed the relationships between the measures of overall host physiology, immunity and parasite load when temporal variability effect was removed. When analyzing separately parasite groups with different life-strategies, we found that fish with a worse condition status were infected more by monogeneans, representing the most abundant parasite group. The high infection by cestodes seems to activate the phagocytes. A weak relationship was found between spleen size and abundance of trematodes when taking into account seasonal changes. Conclusions Even if no direct trade-off between the measures of host immunity and physiology was confirmed when taking into account the seasonality, it seems that seasonal variability affects host immunity and physiology through energy allocation in a trade-off between life important

  12. Reproductive and Endocrine Biomarkers in Largemouth Bass (Micropterus salmoides) and Common Carp (Cyprinus carpio) from United States Waters

    Science.gov (United States)

    Goodbred, Steven L.; Smith, Stephen B.; Greene, Patricia S.; Rauschenberger, Richard H.; Bartish, Timothy M.

    2007-01-01

    A nationwide reconnaissance investigation was initiated in 1994 to develop and evaluate a suite of reproductive and endocrine biomarkers for their potential to assess reproductive health and status in teleost (bony) fish. Fish collections were made at 119 sites, representing many regions of the country and land- and water-use settings. Collectively, this report will provide a national and regional benchmark and a basis for evaluating biomarkers of endocrine and reproductive function. Approximately 2,200 common carp (Cyprinus carpio) and 650 largemouth bass (Micropterus salmoides) were collected from 1994 through 1997. The suite of biomarkers used for these studies included: the plasma sex-steroid hormones, 17?-estradiol (E2) and 11 ketotestosterone (11KT); the ratio of E2 to 11KT (E2:11KT); plasma vitellogenin (VTG); and stage of gonadal development. This data report provides fish size, stage and reproductive biomarker data for individual fish and for site and regional summaries of these variables.

  13. Expression of immune system-related genes during ontogeny in experimentally wounded common carp (Cyprinus carpio) larvae and juveniles

    DEFF Research Database (Denmark)

    Schmidt, Jacob; Nielsen, Michael Engelbrecht

    2014-01-01

    We investigated the effect of full-thickness incisional wounding on expression of genes related to the immune system in larvae and juveniles of common carp (Cyprinus carpio). The wounds were inflicted by needle puncture immediately below the anterior part of the dorsal fin on days 7, 14, 28 and 49...... after fertilization. We followed the local gene expression 1, 3 and 7days after wounding by removing head and viscera before extracting RNA from the remaining part of the fish, including the wound area. In addition, we visually followed wound healing. Overall the wounds had regenerated to a point where...... the investigated genes as a result of the wounding. HSP70 was downregulated 1 and 3days post-wounding in the smallest larvae. However, HSP70 was differentially expressed at different time-points in a similar manner in wounded and mock-wounded groups, thus suggesting a stress effect of the handling...

  14. Profilings of MicroRNAs in the Liver of Common Carp (Cyprinus carpio Infected with Flavobacterium columnare

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    Lijuan Zhao

    2016-04-01

    Full Text Available MicroRNAs (miRNAs play important roles in regulation of many biological processes in eukaryotes, including pathogen infection and host interactions. Flavobacterium columnare (FC infection can cause great economic loss of common carp (Cyprinus carpio which is one of the most important cultured fish in the world. However, miRNAs in response to FC infection in common carp has not been characterized. To identify specific miRNAs involved in common carp infected with FC, we performed microRNA sequencing using livers of common carp infected with and without FC. A total of 698 miRNAs were identified, including 142 which were identified and deposited in the miRbase database (Available online: http://www.mirbase.org/ and 556 had only predicted miRNAs. Among the deposited miRNAs, eight miRNAs were first identified in common carp. Thirty of the 698 miRNAs were differentially expressed miRNAs (DIE-miRNAs between the FC infected and control samples. From the DIE-miRNAs, seven were selected randomly and their expression profiles were confirmed to be consistent with the microRNA sequencing results using RT-PCR and qRT-PCR. In addition, a total of 27,363 target genes of the 30 DIE-miRNAs were predicted. The target genes were enriched in five Kyoto Encyclopedia of Genes and Genomes (KEGG pathways, including focal adhesion, extracellular matrix (ECM-receptor interaction, erythroblastic leukemia viral oncogene homolog (ErbB signaling pathway, regulation of actin cytoskeleton, and adherent junction. The miRNA expression profile of the liver of common carp infected with FC will pave the way for the development of effective strategies to fight against FC infection.

  15. Involvement of the mitfa gene in the development of pigment cell in Japanese ornamental (Koi) carp (Cyprinus carpio L.).

    Science.gov (United States)

    Liu, J H; Wen, S; Luo, C; Zhang, Y Q; Tao, M; Wang, D W; Deng, S M; Xiao, Y M

    2015-01-01

    A colored phenotype is an important feature of ornamental fish. In mammals, microphthalmia-associated transcription factor (MITF) was found to regulate the development of melanocytes. In this study, the mitfa cDNA was first cloned from the Japanese ornamental (Koi) carp (Cyprinus carpio L.), an important ornamental freshwater fish. The full-length cDNA of the mitfa gene contains 1634 bp, coding for 412 amino acids in Koi. The identity degree of mitfa amino acid sequences between the Koi carp and zebrafish is 92.9%. We tested the expression of the mitfa gene in several varieties of Koi using reverse transcription-polymerase chain reaction and found that the mitfa gene is highly expressed in the skin tissues of the Taisho sanke and the Procypris merus. Interestingly, the mitfa gene was also expressed in the Kohaku and Yamabaki ogon, although melanocytes were not observed in the skin. Koi carp embryos were transparent and colorless, while after hatching, different types of pigment cells successively emerged in a fixed order. In Taisho sanke, melanocytes first appeared in the trunk at approximately 12 days of age. Subsequently, there was a large area of melanocytes by 30 days of age. The expression level of the mitfa mRNA was low in early embryos and newly hatched larvae, and increased to high levels in 30-day-old fry. The results show that the mitfa gene is involved in regulating fish body color in the development of both melanocytes and pigment cells. PMID:25867426

  16. Toxicity Testing and the Effect of Landfill Leachate in Malaysia on Behavior of Common Carp (Cyprinus carpio L., 1758; Pisces, Cyprinidae)

    OpenAIRE

    Jaffar Y.M. Alkassasbeh; Lee Y. Heng; Salmijah Surif

    2009-01-01

    Problem Statement: Landfill leachate had been implicated in environmental pollution, developmental anomalies, birth defect and surface and groundwater pollution worldwide. This study has been conducted to determine the toxicity of landfill leachate from three different landfills in Malaysia on fry common carp (Cyprinus carpio L., 1758; Pisces, Cyprinidae) in combination with some physico-chemical parameters analysis. Approach: Leachates were collected from Air Hitam (AHL), Ampar Tenang (ATL) ...

  17. Biological significance of [14C]phenol accumulation in different organs of a murrel, Channa punctatus, and the common carp, Cyprinus carpio

    International Nuclear Information System (INIS)

    Phenol, a ubiquitous component of industrial effluents, is a common pollutant of water resources and a serious threat to fish. The present work demonstrates that a significant amount of phenol is retained by various tissues of the common carp, Cyprinus carpio, and the snake-headed murrel, Channa punctatus. The rate of [14C]phenol accumulation was higher carp than in the murrel. It is suggested that retention of phenol in the brain and ovary may seriously affect the reproductive potential of the fish

  18. The response of thyroid hormones, biochemical and enzymological biomarkers to pyrene exposure in common carp (Cyprinus carpio).

    Science.gov (United States)

    Shirdel, Iman; Kalbassi, Mohammad Reza; Shokri, Milad; Olyaei, Roya; Sharifpour, Issa

    2016-08-01

    Polycyclic Aromatic Hydrocarbons (PAHs) are discharged into aquatic environments through anthropogenic activities mainly industrial and municipal effluents. There is little information on the adverse effects of pyrene, a member of the PAH family which is classified as a priority pollutant by the USEPA, on fish biochemical and physiological endpoints, particularly thyroid hormones. The present study investigated the effects of subacute semi-static pyrene exposure on biochemical, enzymological and ionoregulatory responses as well as thyroid hormones in common carp (Cyprinus carpio). The fish (140±10g, 1(+) year) were exposed to 10, 50 and 100µg/l nominal concentrations of pyrene for 35 days. The results revealed that pyrene at these concentrations significantly altered plasma levels of glucose, cholesterol, triglyceride, total protein, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). Moreover, plasma thyroid hormones (T3 and T4) were significantly decreased in fish exposed to pyrene. In contrast, plasma electrolytes (sodium, potassium and calcium) levels remained statistically unchanged after exposure to the various pyrene concentrations. In conclusion, the studied biomarkers may be used as monitoring tools to evaluate pyrene toxicity. Pyrene induced diverse effects on the physiological endpoints of common carp, thus this chemical should be considered in toxicity studies concerning PAHs. Furthermore, this study confirmed that there was an interaction between pyrene and the thyroid system in fish. Therefore, the thyroid system may be used to assess the impact of pyrene on fish. PMID:27123973

  19. The accumulation and distribution of 60Co in carp (Cyprinus carpio) in water-fish compartment system

    International Nuclear Information System (INIS)

    In relation with nuclear safety assessment, the parameter of radionuclide transfer in the environment is significantly needed for internal doses estimation received by public trough environment - food product - human pathways. International Atomic Energy Agency (IAEA) has published the transfer parameter data for temperate zone in Technical Report Series 472. In order to complete the IAEA data, especially for tropical region, the accumulation and distribution of 60Co in carp (Cyprinus carpio) was experimentally studied based on the water-fish compartment system. The carp were cultured in 500L water containing 60Co of about 30 Bq.mL-1 in a water tank. The 60Co concentration in fish and water were measured using gamma spectrometer. The 60Co was accumulated and distributed in the fish tissues with the concentration ratio (CR) of 3.08 mL.g-1 1.55 mL.g-1 and 1.14 mL.g-1 for internal organs, bones, and muscle, respectively. The CR of 60Co in the fish will be useful in internal radiation dose estimation to human trough water-fish-human pathway, and will also complete the IAEA transfer parameter data for tropical region. (author)

  20. Seasonal Variations Of Heavy Metals In Common Carp (Cyprinus Carpio L., 1758 Collected From Sikkak Dam Of Tlemcen (Algeria

    Directory of Open Access Journals (Sweden)

    Derrag Zineb,

    2014-01-01

    Full Text Available Seasonal variations in the concentrations of six heavy metals (Cd, Fe, Cu, Pb, Zn, and Ni were determined from December 2010 to November 2011 in four different tissues (muscles, gills, gonads, and livers of common carp (Cyprinus carpio L., 1758 from Sikkak dam at Ainyoucef (Wilaya of Tlemcen which is an important water source for irrigation and drinking in northwest part of Algeria. Heavy metals in fish samples were analyzed by atomic absorption spectrophotometry (AAS after dry digestion. One-way ANOVA and principal component analysis (PCA were used to compare the data among seasons (level of 0,05. Mean concentrations were found to decrease in sequence of Cyprinuscarpio samples, in muscle and liver as Fe > Zn >Pb> Ni > Cu > Cd, in gills as Zn > Fe >Pb> Ni > Cu > Cd, and in gonads Fe > Zn > Ni >Pb> Cu > Cd. In samples Cd, Pb and Zn concentrations exceeded the tolerable values provided by FAO/WHO. The highest metals concentrations were found in liver followed by gills, by gonads, and by muscle. Heavy metal levels in tissues of carp were decreased in winter. The obtained results showed that the average values of Cd, Cu, Zn and Ni were at the highest levels in summer. The highest Pb levels were measured in the spring and Fe in autumn. It found that all tested metals in organs did not reveal any significant difference between different seasons (P > 0,05, (inter-season comparison.

  1. Antimicrobial effects of guava leaf (Psidium guajava Linn. extract against Aeromonas hydrophila in fancy carp (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Tangtongpirot, J.

    2007-03-01

    Full Text Available The objective of this study was to determine the effects of guava leaves in treatment or prevention of bacterial infection using methanol extracted guava leaves. Three hundred and eighty four fancy carps (Cyprinus carpio with average weight of 25.5 g. were acclimated for 14 days before the experiment. Fish were divided into 32 groups of two replicates each with 6 fish. All experiments were done in replicate. Guava leaves were macerated and extracted by methanol distillation and evaporation to produce 12.99% of dried leave weight. The exposures were divided into oral route using 5% (MIC and 10 % (2xMIC. And 1000 ppm (MIC and 2000 ppm (2xMIC for dip and bath methods. MIC by agar dilution method was 1000 ppm. At the 1000 ppm concentration dipped for 5 minutes, fish lost consciousness but this was reversible when returned to freshwater, which may due to the antinociceptive effect. All fish died when dipped at 2000 ppm concentration. The relative percent survival (RPS of 5% feed mix group was significantly higher than the 10% feed mix group and higher when fed for longer time. All groups receiving guava leaf extract had significantly higher percent phagocytosis and percent chemotaxis than the controls (P<0.05. The results indicated that guava leaf extract can stimulate the non-specific immune responses and decrease the mortality rate of the bacterial infected carp. The effects were enhanced by the longer period of exposure.

  2. Toxic effects, bioconcentration and depuration of verapamil in the early life stages of common carp (Cyprinus carpio L.)

    Energy Technology Data Exchange (ETDEWEB)

    Steinbach, Christoph, E-mail: steinbach@frov.jcu.cz [Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, CZ-38925 Vodnany (Czech Republic); Fedorova, Ganna [Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, CZ-38925 Vodnany (Czech Republic); Prokes, Miroslav [Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, v.v.i., Kvetna 8, 603 65 Brno (Czech Republic); Grabicova, Katerina; Machova, Jana; Grabic, Roman; Valentova, Olga; Kroupova, Hana Kocour [Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, CZ-38925 Vodnany (Czech Republic)

    2013-09-01

    Verapamil is a pharmaceutical that belongs to a group of calcium channel blockers and is mainly used as a treatment of angina pectoris and arterial hypertension. Verapamil has been detected in aquatic environments in concentrations ranging from ng L{sup −1} to μg L{sup −1}. In the present study, a series of acute toxicity tests of verapamil on various developmental stages of common carp (Cyprinus carpio) were conducted. As a result, 96hLC{sub 50} values of verapamil were estimated at 16.4 ± 9.2, 7.3 ± 1.5 and 4.8 ± 0.2 mg L{sup −1} for embryos (E5–E9) and common carp larvae L2 and L5, respectively. Lethal concentrations of verapamil decreased with an increase in the age of the fish. Acute exposure to verapamil significantly reduced the heart rate in the embryos and larvae. In an embryo-larval toxicity test (sub-chronic exposure), the bioconcentration, depuration, and toxic effects of verapamil were assessed in common carp. The fish were exposed to verapamil in a concentration of 0.463 (environmentally relevant), 4.63, 46.3 and 463 μg L{sup −1}. Verapamil had no effect on the accumulated mortality, hatching, condition factor, growth or ontogeny of the fish in any of the tested concentrations. In carp exposed to 463 and 46.3 μg L{sup −1} of verapamil, significantly higher occurrences of malformations and edemas were observed compared to the control. The bioconcentration factor of verapamil in whole fish homogenates ranged between 6.6 and 16.6 and was therefore below the critical value for hazard substances (BCF > 500). The half-life and the 95% depuration time for the tested compound were estimated to be 10.2 ± 1.6 days and 44.2 ± 8.6 days, respectively. No effects of verapamil on the studied endpoints were observed at environmentally relevant concentrations. - Highlights: • Study of the acute and sub-chronic toxicity of verapamil on early-life stages of common carp. • Acute exposure to verapamil reduced the heart rate in early-life stages of

  3. Toxic effects, bioconcentration and depuration of verapamil in the early life stages of common carp (Cyprinus carpio L.)

    International Nuclear Information System (INIS)

    Verapamil is a pharmaceutical that belongs to a group of calcium channel blockers and is mainly used as a treatment of angina pectoris and arterial hypertension. Verapamil has been detected in aquatic environments in concentrations ranging from ng L−1 to μg L−1. In the present study, a series of acute toxicity tests of verapamil on various developmental stages of common carp (Cyprinus carpio) were conducted. As a result, 96hLC50 values of verapamil were estimated at 16.4 ± 9.2, 7.3 ± 1.5 and 4.8 ± 0.2 mg L−1 for embryos (E5–E9) and common carp larvae L2 and L5, respectively. Lethal concentrations of verapamil decreased with an increase in the age of the fish. Acute exposure to verapamil significantly reduced the heart rate in the embryos and larvae. In an embryo-larval toxicity test (sub-chronic exposure), the bioconcentration, depuration, and toxic effects of verapamil were assessed in common carp. The fish were exposed to verapamil in a concentration of 0.463 (environmentally relevant), 4.63, 46.3 and 463 μg L−1. Verapamil had no effect on the accumulated mortality, hatching, condition factor, growth or ontogeny of the fish in any of the tested concentrations. In carp exposed to 463 and 46.3 μg L−1 of verapamil, significantly higher occurrences of malformations and edemas were observed compared to the control. The bioconcentration factor of verapamil in whole fish homogenates ranged between 6.6 and 16.6 and was therefore below the critical value for hazard substances (BCF > 500). The half-life and the 95% depuration time for the tested compound were estimated to be 10.2 ± 1.6 days and 44.2 ± 8.6 days, respectively. No effects of verapamil on the studied endpoints were observed at environmentally relevant concentrations. - Highlights: • Study of the acute and sub-chronic toxicity of verapamil on early-life stages of common carp. • Acute exposure to verapamil reduced the heart rate in early-life stages of common carp. • The

  4. Sub-lethal toxicity of chlorpyrifos on Common carp, Cyprinus carpio (Linnaeus, 1758: Biochemical response

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    Mahdi Banaee

    2014-01-01

    Full Text Available Chlorpyrifos, an organophosphate pesticide, is widely used to control pests in agriculture farms and orchards of fruit trees. In this study, the fish were exposed to sub-lethal concentrations of chlorpyrifos which were determined based on numerical value of 96 h LC50. Blood was sampled after 10, 20 and 30 days and biochemical parameters including glucose, total protein, albumin, globulin, triglyceride and cholesterol levels, and aspartate aminotransferase (AST, alanine aminotransferase (ALT, lactate dehydrogenase (LDH, creatine kinase (CK, alkaline phosphatase (ALP and acetylcholinsetrase (AChE activities were measured. Behavioral changes in the fish were also recorded during the experiment. Unbalanced swimming, swimming in the surface water and hyperglycemia, increased blood triglyceride, and increased levels of AST, LDH and CK activities as well as decreased levels of AChE activity were important changes that were observed in the specimens exposed to chlorpyrifos during experimental periods. The most important alterations in the blood biochemical parameters were measured in the specimens exposed to 40 µg/L chlorpyrifos on the 20th and 30th day of the trial. In conclusion, results of the present study indicated that exposure to sub-lethal concentrations of chlorpyrifos as low as 40 µg/L may cause biochemical and behavioral changes in Cyprinus carpio.

  5. Quality changes and predictive models of radial basis function neural networks for brined common carp (Cyprinus carpio) fillets during frozen storage.

    Science.gov (United States)

    Kong, Chunli; Wang, Huiyi; Li, Dapeng; Zhang, Yuemei; Pan, Jinfeng; Zhu, Beiwei; Luo, Yongkang

    2016-06-15

    To investigate and predict quality of 2% brined common carp (Cyprinus carpio) fillets during frozen storage, free fatty acids (FFA), salt extractable protein (SEP), total sulfhydryl (SH) content, and Ca(2+)-ATPase activity were determined at 261K, 253K, and 245K, respectively. There was a dramatic increase (Pfirst 3weeks. SEP decreased to 67.31% after 17weeks at 245K, whereas it took about 7weeks and 13weeks to decrease to the same extent at 261K and 253K, respectively. Ca(2+)-ATPase activity kept decreasing to 18.28% after 7weeks at 261K. Furthermore, radial basis function neural networks (RBFNNs) were developed to predict quality (FFA, SEP, SH, and Ca(2+)-ATPase activity) of brined carp fillets during frozen storage with relative errors all within ±5%. Thus, RBFNN is a promising method to predict quality of carp fillets during storage at 245-261K. PMID:26868584

  6. 鲤鱼微卫星分子标记的筛选%Isolation of Microsatellite Markers in the Common Carp (Cyprinus carpio)

    Institute of Scientific and Technical Information of China (English)

    魏东旺; 楼允东; 孙效文; 沈俊宝

    2001-01-01

    A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA)15 probe labelled at the 5 ' end with γ32P-ATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR on Cyprinus carpio haematopterus was carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.

  7. Obtención, caracterización microbiológica y físico-química de ensilado biológico de carpa (Cyprinus carpio - Obtaining, characterization microbiological and physic-chemical of carp biological silage (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Fernández Herrero, Adriana L.

    2011-08-01

    Full Text Available ResumenEl propósito del presente estudio fue determinar los cambios en la calidad nutricional y composición química que ocurren durante el ensilado de desechos de carpa (Cyprinus carpio, y determinar cual de las dos proporciones de miel: yogur es la apropiada para su utilización como fuente proteica en la alimentación animal.SummaryThe purpose of the present study was to determine the changes in the nutritional quality and the chemical composition that occur during the ensilage of waste carp (Cyprinus carpio and establish which of the two honey proportions:

  8. Oxidative stress and immune disturbance after long-term exposure to bisphenol A in juvenile common carp (Cyprinus carpio).

    Science.gov (United States)

    Qiu, Wenhui; Chen, Jingsi; Li, Yijie; Chen, Zhong; Jiang, Lihui; Yang, Ming; Wu, Minghong

    2016-08-01

    Bisphenol A (BPA) is a well-known endocrine disrupting chemical (EDC), ubiquitous in the aquatic environment, which poses an ecotoxicological risk to the health of aquatic organisms. However, the immunotoxic effects of its long-term exposure on fish have received limited attention. We examined a number of typical immune-related parameters and oxidative stress indices in the liver and blood serum of the red common carp (Cyprinus carpio), following a 30-day exposure to five different concentrations of BPA (0.1, 1, 10, 100, and 1000μg/L). A significant increase in the hepato somatic index was observed in fish upon exposure to 1000µg/L BPA, which correlated strongly with the accumulated BPA concentrations in fish bile. Induced oxidative stress was also apparent in the exposed fish liver, based on the enhanced levels of lipid peroxidation and inhibited activities of catalase, superoxide dismutase, and glutathione peroxidase. Serum lysozyme and C-reaction protein levels increased at low concentrations of exposure; however, they were significantly suppressed upon exposure to high concentrations. A significant increase was observed in the levels of immunoglobulin M, complement component 3, and alkaline phosphatase, in both fish liver and serum at low doses of 0.1 and 1μg/L. This suggests that long-term exposure to environmentally relevant concentrations of BPA (even as low as 0.1μg/L) could significantly disturb the immune response of fish. Moreover, RXRα expression in the liver was significantly altered upon BPA exposure and the trend underlying this change correlated closely with those of the most immune-related parameters, implying the involvement of the PPARγ/RXRα signaling pathway in regulating the immune response of fish upon long-term BPA exposure. In short, our results demonstrate the susceptibility of fish immune system to long-term BPA exposure. Therefore, the immunotoxicity of EDCs in aquatic organisms should not have been underestimated. PMID:27088622

  9. Effect of Terbuthylazine-2-hydroxy at Environmental Concentrations on Early Life Stages of Common Carp (Cyprinus carpio L.

    Directory of Open Access Journals (Sweden)

    Josef Velisek

    2014-01-01

    Full Text Available The aim of the study was to investigate effects of the triazine’s herbicide terbuthylazine-2-hydroxy on early life stage of common carp (Cyprinus carpio L. through antioxidant indices, mortality, growth, development, and histopathology. Based on accumulated mortality in the experimental groups, lethal concentrations of terbuthylazine-2-hydroxy were estimated at 35-day LC50 = 10.9 mg/L terbuthylazine-2-hydroxy. By day 15, fish were exposed to 3.5 mg/L and by day 26, fish were exposed to 0.0029 mg/L; real environmental concentration in Czech rivers, 0.07 mg/L, 1.4 mg/L, and 3.5 mg/L terbuthylazine-2-hydroxy, showed significantly lower mass and total length compared with controls. Based on inhibition of growth in the experimental groups, lowest observed effect concentration (LOEC = 0.002 mg/L terbuthylazine-2-hydroxy and no observed effect concentration (NOEC = 0.0001 mg/L terbuthylazine-2-hydroxy. No significant negative effects on hatching or embryo viability were demonstrated at the concentrations tested, but significant differences in early ontogeny among groups were noted. Fish from the two highest tested concentrations showed a dose-related delay in development compared with the controls. Total superoxide dismutase (SOD activity was significant lower in all groups testedly for terbuthylazine-2-hydroxy compared with the control group. At concentrations of 1.4 and 3.5 mg/L damage to caudal kidney tubules when compared to control fish was found.

  10. Vitellogenesis in Archigetes sieboldi Leuckart, 1878 (Cestoda, Caryophyllidea, Caryophyllaeidae), an intestinal parasite of carp (Cyprinus carpio L.).

    Science.gov (United States)

    Brunanská, M; Mackiewicz, J S; Nebesárová, J

    2012-12-01

    Vitellogenesis in the caryophyllidean tapeworm Archigetes sieboldi Leuckart, 1878, from carp Cyprinus carpio L. in Slovakia, has been examined using transmission electron microscopy and cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP) for glycogen. Vitelline follicles extend in two lateral bands in the medullary parenchyma along both sides of the monozoic body. They are surrounded by an external basal lamina and contain vitellocytes and an interstitial tissue. The general pattern of vitellogenesis is essentially like that of other caryophyllideans. It involves four stages: immature, early maturing, advanced maturing cells and mature vitellocytes. During vitellogenesis, a continuous increase in cell volume is accompanied by an extensive development of cell components engaged in shell globule formation, e.g. granular endoplasmic reticulum and Golgi. Shell globule clusters are membrane-bound. Nuclear and nucleolar transformation are associated with formation and storage of large amounts of intranuclear glycogen, a very specific feature of the Caryophyllidea. For the first time, (a) additional vitelline material in Archigetes is represented by lamellar bodies and (b) lipid droplets are described in the mature vitellocytes from vitelline follicles and vitelloduct of the Caryophyllidea. Our results indicate that there may be a double origin of lamellar bodies: either from the endoplasmic reticulum or through transformation of shell globule/shell globule clusters. Lamellar body clusters and some single lamellar bodies appear to have a membrane. Other ultrastructural features of vitellogenesis and/or vitellocyte in A. sieboldi from its vertebrate (fish) and invertebrate (oligochaete) hosts are briefly compared and contrasted with those in other caryophyllideans and/or Neodermata. PMID:23059891

  11. Heavy Metal Concentrations in Water, Sediments and Common Carp (Cyprinus carpio Fish Species from Lake Naivasha, Kenya

    Directory of Open Access Journals (Sweden)

    G.M. Ogendi

    2014-08-01

    Full Text Available Heavy metals are potentially toxic to organisms causing lethal and sub lethal effects. Above threshold levels, these metals have been shown to adversely affect both aquatic and terrestrial organisms. Human health may also be impacted negatively through consumption of metal-contaminated foods and water. Lake Naivasha, Kenya, like many freshwater bodies in developing countries is a source of fish and water for human consumption in Naivasha town and its environs. Ironically, the lake receives untreated floricultural, agricultural and industrial effluents from its catchment area. In the recent past, there have been unconfirmed reports that fish from this lake are contaminated with heavy metals mainly Cu, Hg, Cd and Pb to levels that are harmful to humans. It is against this background that this study was conducted to determine the sources of the aforementioned heavy metals in the lake, their concentrations in water and sediments and in common carp (Cyprinus carpio that constitutes a major portion of the Lake Naivasha fishery. Water, sediment and fish samples were collected from purposefully selected sites in six sampling occasions during both the dry and wet seasons. Analyses for heavy metals in fish, sediment and water samples were done using an Atomic Absorption Spectrophotometer (AAS. The study findings indicate that the heavy metal concentrations in the water and sediments collected from impacted sites were significantly higher (p<0.05 compared to those from unexpected sites. In spite of this, the metal concentrations in water were significantly lower than those recommended by the WHO and USEPA as drinking water guideline values. Metal concentrations in C. carpio were also significantly lower (p<0.05 than the World Health Organization (WHO recommended levels for fish intended for human consumption. Based on these findings, it is safe to conclude that drinking water and fish collected from Lake Naivasha do not pose immediate health risks to human

  12. (Uncommon) Mechanisms of Branchial Ammonia Excretion in the Common Carp (Cyprinus carpio) in Response to Environmentally Induced Metabolic Acidosis.

    Science.gov (United States)

    Wright, Patricia A; Wood, Chris M; Hiroi, Junya; Wilson, Jonathan M

    2016-01-01

    Freshwater fishes generally increase ammonia excretion in acidic waters. The new model of ammonia transport in freshwater fish involves an association between the Rhesus (Rh) protein Rhcg-b, the Na(+)/H(+) exchanger (NHE), and a suite of other membrane transporters. We tested the hypothesis that Rhcg-b and NHE3 together play a critical role in branchial ammonia excretion in common carp (Cyprinus carpio) chronically exposed to a low-pH environment. Carp were exposed to three sequential environmental treatments-control pH 7.6 water (24 h), pH 4.0 water (72 h), and recovery pH 7.6 water (24 h)-or in a separate series were simply exposed to either control (72 h) or pH 4.0 (72 h) water. Branchial ammonia excretion was increased by ∼2.5-fold in the acid compared with the control period, despite the absence of an increase in the plasma-to-water partial pressure NH3 gradient. Alanine aminotransferase activity was higher in the gills of fish exposed to pH 4 versus control water, suggesting that ammonia may be generated in gill tissue. Gill Rhcg-b and NHE3b messenger RNA levels were significantly elevated in acid-treated relative to control fish, but at the protein level Rhcg-b decreased (30%) and NHE3b increased (2-fold) in response to water of pH 4.0. Using immunofluorescence microscopy, NHE3b and Rhcg-b were found to be colocalized to ionocytes along the interlamellar space of the filament of control fish. After 72 h of acid exposure, Rhcg-b staining almost disappeared from this region, and NHE3b was more prominent along the lamellae. We propose that ammoniagenesis within the gill tissue itself is responsible for the higher rates of branchial ammonia excretion during chronic metabolic acidosis. Unexpectedly, gill Rhcg-b does not appear to be important in gill ammonia transport in low-pH water, but the strong induction of NHE3b suggests that some NH4(+) may be eliminated directly in exchange for Na(+). These findings contrast with previous studies in larval zebrafish

  13. Experimental Evidence for the Effects of Common Carp (Cyprinus carpio L., 1758 on Freshwater Ecosystems: A Narrative Review with Management Directions for Turkish Inland Waters

    Directory of Open Access Journals (Sweden)

    Lorenzo VILIZZI

    2015-12-01

    Full Text Available The management of common carp Cyprinus carpio has become a priority issue in most of its native range and where it has been introduced. This is because of the effects (both documented and anecdotal on freshwater ecosystems that the species is able to exert. To provide a general framework for future management of introductions of this species, this study presents a near-comprehensive, “narrative” review (complementing a “systematic” review: Vilizzi, Tarkan, Copp 2015 of experimental studies of the impacts of common carp spanning almost nine decades. Based on 139 experiments presenting results for a total of 400 “assessments” aimed at evaluating the effects of C. carpio on selected ecological components, a conceptual model linking both abiotic (i.e. turbidity/suspended solids, nitrogen, phosphorus and biotic components (i.e. phytoplankton/chlorophyll a, aquatic macrophytes, zooplankton, benthic invertebrates, amphibians, waterfowl, fish was refined. Given the status of C. carpio as a species of low concern in Turkish inland waters and its overall unsuccessful recruitment in stocked reservoirs, in the light of the present findings it is suggested that environmental managers should consider targeting shallow (natural lakes for successful fisheries yields, but conditional upon careful assessment of the economic benefits vs. ecological risks involved.

  14. Cyprinid herpesvirus-3 (CyHV-3) disturbs osmotic balance in carp (Cyprinus carpio L.)--A potential cause of mortality.

    Science.gov (United States)

    Negenborn, J; van der Marel, M C; Ganter, M; Steinhagen, D

    2015-06-12

    Cyprinid herpesvirus-3 (CyHV-3) causes a fatal disease in carp (Cyprinus carpio) and its ornamental koi varieties which seriously affects production and trade of this fish species globally. Up to now, the pathophysiology of this disease remains unclear. Affected individuals develop most prominent lesions in gills, skin and kidney, in tissues which are involved in the osmotic regulation of freshwater teleosts. Therefore, here serum and urine electrolyte levels were examined during the course of an experimental infection of carp with CyHV-3. In infected carp an interstitial nephritis with a progressive deterioration of nephric tubules developed, which was paralleled by elevated electrolyte losses, mainly Na(+) in the urine. The urine/plasma ratio for Na(+) increased from 0.03 in uninfected carp to 0.43-0.83 in carp under CyHV-3 infection, while concentration of divalent ions were not significantly changed. These electrolyte losses could not be compensated since plasma osmolality and Na(+) concentration dropped significantly in CyHV-3 infected carp. This was most probably caused by the progressive deterioration of the branchial epithelium, which in teleosts plays a prominent role in osmoregulation, and which was seen concomitantly with decreasing electrolyte levels in the serum of carp under CyHV-3 infection. Immediately after infection with CyHV-3, by day 2 post exposure, affected carp showed severe anaemia and prominent leucocytosis indicating the development of an acute inflammation, which could intensify the observed hydro-mineral imbalances. The data presented here show that an infection with CyHV-3 induces an acute inflammation and a severe dysfunction of osmoregulation in affected carp or koi, which may lead to death in particular in the case of acute disease progression. PMID:25888311

  15. ANALYSIS OF INDIVIDUAL BIOLOGICAL CHARACTERISTICS OF AMUR CARP (CYPRINUS CARPIO HAEMATOPTERUS REPRODUCED USING CRYOPRESERVED SPERM

    Directory of Open Access Journals (Sweden)

    N. Kolisnyk

    2014-12-01

    Full Text Available Purpose. To reproduce Amur carp population using cryopreserved sperm and analyze some biological and fish culture peculiarities of the reproduced fish stock. Methodology. Generally accepted methods for fish culture [1]. Experimental reproduction was carried out in pond conditions of «Carpathian vodogray» LTD (Lisnevychi village, Pustomytivsky district, Lviv region. Hydrochemical analysis was carried out classically by O. Alуokin (1970 [2], hydrobiological studies in the fatting ponds according to V. Zhadin (1956, 1960 [3, 4]. Haemoglobin concentration was determined by hemocyanin method of G. Dervis, A. Vorobiov [5]. Blood for this method was collected from fish heart with the use of Pasteur pipettes in Eppendorf tubes with heparin. Following exterior morphometric parameters were analysed: body weight (m, g, standard fish body length (l, cm, largest body height (H, cm and body circumference (O cm. Following exterior indices were calculated based on these parameters: body depth index (l/H, body circumference index (l/O and Fulton’s condition factor (Kv. The study was carried out using two groups of carp: control and experimental. The first group was reproduced from the native sperm, the second from the cryopreserved sperm. Findings. Carp reproduction and growing was carried out using native and cryopreserved sperm. This work contains the results of growing 1+ Amur carp of experimental and control groups. Hydrochemical and hydrobiological parameters of the fattening ponds were studied. Peculiarities of the exterior and some hematological parameters of the carp of different origin were characterized. Originality. For the first time we performed a comparison of some biological parameters of Amur carp reproduced using native and cryopreserved sperm. Practical Value. Considering the economic importance of Amur carp due to its use in hybridization, reproduction of its population plays an important role in the development of the stocks of the pure

  16. Effects of dietary fructo-oligosaccharide supplementation on the growth performance, haemato-immunological parameters, gut microbiota and stress resistance of common carp (Cyprinus carpio) fry.

    Science.gov (United States)

    Hoseinifar, Seyed Hossein; Soleimani, Narges; Ringø, Einar

    2014-10-28

    The present study was conducted to investigate the effects of dietary fructo-oligosaccharide (FOS) (0, 1, 2 and 3%) supplementation on the growth performance, haemato-immunological parameters, cultivable autochthonous (non-adherent) intestinal microbiota and stress resistance of common carp (Cyprinus carpio) fry (3·23 (SEM 0·14) g). These parameters were measured after feeding the carp fry with the experimental diets for 7 weeks. Dietary FOS supplementation had no significant effects on the growth performance and food intake of carp fry compared with the control treatment. It also had no significant effects on the following haematological parameters: erythrocyte count; leucocyte counts (WBC); haematocrit; Hb; mean corpuscular volume; mean corpuscular Hb content; mean corpuscular Hb concentration. However, WBC and respiratory burst activity were significantly affected by dietary FOS supplementation. Evaluation of the cultivable autochthonous intestinal microbiota revealed a significant increase in the levels of total viable heterotrophic aerobic bacteria and lactic acid bacteria in fish fed diets supplemented with 2 and 3% FOS. Furthermore, dietary FOS supplementation significantly increased the survival rate and stress resistance of carp fry compared with the control treatment. These results encourage conducting further research on the administration of FOS and other prebiotics in carp fry studies. PMID:25313574

  17. DRESSING PERCENTAGE OF FOUR CROATIAN COMMON CARP (CYPRINUS CARPIO L. POPULATIONS

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    Roman Safner

    2001-12-01

    Full Text Available The morphometric traits were determined on the sample of 459 marketable size common carp from the four Croatian fish farms. There traits were analyzed by the classical methods in order to determine the influence of population, scaliness, age and sex on the dressing percentage. The obtained results show the existence of different phenotypes of common carps at Croatian farms, primarily, morphometric differences. However, the sygnificant diferences of dressing percentage among the populations were not registered (p>0.05. The significant difference between the dressing percentages of scaled and mirror carps was not detected (p>0.05, what leads to the conclusion that the scaliness does not influence dressing percentage significantly. The age and sex also, dit not show significant influence on dressing percentage. As the carps were sampled in winter the differences in dressing percentage caused by the different maturation of males and females were excluded.

  18. Bioaccumulation of metals in common carp (Cyprinus carpio L.) from water bodies of Anatolia (Turkey): a review with implications for fisheries and human food consumption.

    Science.gov (United States)

    Vilizzi, Lorenzo; Tarkan, Ali Serhan

    2016-04-01

    Although fish is widely consumed by humans for its nutritional properties, accumulation of heavy metals can pose serious health hazards. Widespread common carp Cyprinus carpio is cultured worldwide and represents an economically important species for fisheries in several countries. These include Turkey, where C. carpio often makes for a large part of the sales of the locally marketed fish and also for a traditional dish. This study provides a review of bioaccumulation of metals in tissues of C. carpio from water bodies of Anatolia and also includes reference to worldwide studies. From 42 water bodies across the region, 27 metals in total were studied, of which Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn were the most widely analysed, mainly in the muscle, liver and gill tissues. Amongst the potentially toxic metals, Cd, Cr and Pb occurred in several water bodies at concentrations not only above maximum allowed limits but also higher relative to other water bodies worldwide, even though As, Hg and Ni were also sometimes present at potentially hazardous concentrations. The essential metals Cu, Fe, Mn, Se and Zn were detected at various concentrations, with the latter two occasionally above limit. All water bodies flagged as having especially critical (i.e. above limit) concentrations of toxic metals supported C. carpio fisheries from highly populated regions, raising concern about food safety and calling for preventative measures. Given the significantly lower bioaccumulation levels in the muscle relative to the liver and gill tissues, it is suggested that consumption of C. carpio as fillets may be safer than after processing into e.g. meat balls and sausages. The limits of 1.0 μg/g for Cr and 1.15 μg/g for Se, currently lacking from the Turkish food safety legislation, are proposed, and it is suggested that a similar meta-analytical approach as adopted in this study may benefit other countries where C. carpio represents an important fisheries resource. PMID:27007291

  19. NUTRITIONAL VALUE OF PLANKTONIC CLADOCERAN DAPHNIA MAGNA FOR COMMON CARP (Cyprinus carpio FRY FEEDING

    Directory of Open Access Journals (Sweden)

    Ivan Bogut

    2010-01-01

    Full Text Available Chemical composition and contents of amino acids and fatty acids in the planktonic cladoceran Daphnia magna were investigated, aiming to evaluate its value for feeding of young carp. Crude protein, crude fat, crude fibre, ash and moisture contents were analyzed according to standard laboratory procedures. Amino acids content was determined by LKB 4101 automatic analyzer and that of fatty acids by Chrompack CP 9000 chromatograph, using a flame ionizing detector. Protein contents amounted to 1.18 and 39.24% of fresh and dry mass, respectively. These amounts of proteins completely meet nutritional requirements both of carp fry and its older categories and other omnivorous fishes. Raw fat and fibre contents in dry weight were 4.98 and 4.32%, respectively, which is suitable for the commercial carp breeding. Methionine and phenylalanine are partially in deficit, whereas other essential amino acids identified in dry mass of Daphnia magna were present in amounts adequate for all carp categories. The proportions of saturated and unsaturated fatty acids in lipids of Daphnia magna were 18.70 and 66.20%, respectively. Among the unsaturated fatty acids, the omega–3 group was present with 27.30%. The omega–3 : omega–6 fatty acids ratio was 5.68:1, which fully meets the carp nutrition requirements.

  20. Ontogeny of the carp (Cyprinus carpio L.) innate immune system: Gene expression and experimental limitations

    DEFF Research Database (Denmark)

    Schmidt, Jacob; Przybylska, Dominika Alicja; Nielsen, Michael Engelbrecht

    2012-01-01

    carp were brought to the facilities at DTU and kept in aquaria at 25°C. They were initially fed Artemia nauplii, and later switched to commercial dry granulate feed. Carp were anaesthetised and then experimentally wounded at days 10, 16, 24, 47 and 94 days post-hatch. Sampling was carried out at day 1......, 3 and 7 post-wounding and samples were stored in RNA later for isolation of RNA. The physical tissue damage was performed using a sterile needle, which penetrated the skin and the underlying musculature in an area above the lateral line of the left side of fish. Carps at the age of 10, 16 and 24...

  1. Toxicity of phthalate esters exposure to carp (Cyprinus carpio) and antioxidant response by biomarker

    OpenAIRE

    Zhao, Xiaoxiang; Gao, Ying; Qi, Mingliang

    2014-01-01

    To study the toxic effects of phthalate esters on the aquatic creatures, carps were exposed to dibutyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP) of six different concentrations for 96 h-LC50 measurements. It shows that the 96 h-LC50 is 16.30 and 37.95 mg L−1, thus the safe concentration (1/10LC50) is 1.63 mg L−1. The activities of xanthine oxidase (XOD) and catalase (CAT) were measured in liver to carp exposure for single or combinations of DBP and DEHP. The quantity of malonic dia...

  2. Investigation of the Selectivity Parameters for Carp (Cyprinus carpio Linnaeus, 1758) in Seyhan Dam Lake

    OpenAIRE

    Özyurt, Caner Enver; Avşar, Dursun

    2005-01-01

    Selectivity studies are very important for sustainable fisheries. Therefore, in this study, selectivity parameters for the carp in Seyhan Dam Lake were estimated using gill nets having a mesh size of 28, 32, 40 and 45 mm. Gill nets used in pikeperch fishing of mesh size of 28, 30 and 32 mm could cause growth in the overfishing of carp. Therefore, it is recommended that longline fishing should be commonly used instead of gill nets on pikeperch fishing. Consequently gill nets having minimum 50 ...

  3. Top-crossing with evaluation of slaughtering value in common carp (Cyprinus carpio L.) offspring

    Czech Academy of Sciences Publication Activity Database

    Gela, David; Rodina, Marek; Linhart, Otomar

    2003-01-01

    Roč. 11, - (2003), s. 379-387. ISSN 0967-6120 R&D Projects: GA ČR GA523/97/S056; GA MŠk ME 403 Institutional research plan: CEZ:AV0Z5045916 Keywords : common carp Subject RIV: ED - Physiology Impact factor: 0.446, year: 2003

  4. ATTEMPTS OF INDUCTION OF SEX-REVERSAL IN CARP (CYPRINUS CARPIO VAR. KOI USING TESTOSTERONE UNDECANOAT

    Directory of Open Access Journals (Sweden)

    A. GROZEA

    2013-12-01

    Full Text Available sexreversalprotocols to obtain inverted males (XX and which are the consequencesusing this hormone for koi carp. To induce sex-reversal, we chose to feed a normalmixed-sex progeny of koi carp with food mixed with 30 (V1, 60 (V2 and 90 (V3 mgTU / kg food, starting at the age of 30 days for a period of 60 days. When fishes had3 months old, they were moved into a bigger aquarium and they were fed withouthormones until the age of 6 months when some of fishes were sacrifices to takesamples for histological studies. At this age the main morphometric traits wereregistered. Our results indicated that the mortality percent raise dependent by thequantity of TU from food, with a maximum value in variant V3 (90 mg TU / kg foodwhere it reached 64% in koi carps until the age of 3 months. Total length was thesingle trait that registered significant differences (p0.01 and p0.05 whencomparisons among control and all the other experimental variants were made. Thissuggests that TU treatment significantly reduced length growing of the carps even itwas administered in dose of 30, 60 or 90 mg / kg food. Supplementation of food withTU modified sex ratio in studied fishes.

  5. Stress and stress disorders in a teleost fish, the common carp Cyprinus carpio L.

    NARCIS (Netherlands)

    Ruane, N.M.

    2002-01-01

    Unlike research using mammalian animal models such as rats or mice, experimental fish often come from wild or commercial sources, leading to a lack of well defined experimental animal models. Isogenic carp offer us a well defined fish model for physiological research. The aim of this thesis was to,

  6. The effect of diazinon on blood plasma biochemistry in carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Lusková, Věra; Svoboda, M.; Kolářová, J.

    Roc. 71, č. 1 (2002), s. 117-123. ISSN 0001-7213 Institutional research plan: CEZ:AV0Z6093917 Keywords : common carp * acute toxicity * haematology Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.370, year: 2002 http://vfu-www.vfu.cz/acta-vet/vol71/pdf/71_117.pdf

  7. Effect of deltamethrin on haematological indices of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Svobodová, Z.; Lusková, Věra; Drastichová, J.; Svoboda, M.; Žlábek, V.

    2003-01-01

    Roč. 72, č. 1 (2003), s. 79-85. ISSN 0001-7213 Institutional research plan: CEZ:AV0Z6093917 Keywords : pyrethroids * acute toxicity * common carp Subject RIV: EG - Zoology Impact factor: 0.336, year: 2003 http://vfu-www.vfu.cz/acta-vet/vol72/pdf/72_079.pdf

  8. The effect of diazinon on haematological indices of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Svoboda, M.; Lusková, Věra; Drastichová, J.; Žlábek, V.

    2001-01-01

    Roč. 70, č. 4 (2001), s. 457-465. ISSN 0001-7213 Institutional research plan: CEZ:MSM 162700004 Keywords : common carp * acute toxicity * haematology Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.274, year: 2001 http://vfu-www.vfu.cz/acta-vet/vol70/pdf/70_457.pdf

  9. Temperature and species-specific effects on ß3-adrenergic receptor cardiac regulation in two freshwater teleosts: Channel catfish (Ictalurus punctatus) and common carp (Cyprinus carpio).

    Science.gov (United States)

    Petersen, L H; Burleson, M L; Huggett, D B

    2015-07-01

    β₃-adrenergic receptors (AR) are important in teleost cardiovascular regulation. To date, it is unknown whether temperature acclimation changes ß₃-AR functionality and consequently the involvement of this AR subtype in teleost cardiac regulation. Common carp (Cyprinus carpio) were acclimated at 12 °C or 23 °C (minimum 3 weeks) after which cardiovascular variables (cardiac output (Q), stroke volume (Sv) and heart rate (fH)) were measured upon injection of the ß₃-AR agonist, BRL(37344), and antagonist, SR(59230A). In both 12 °C and 23 °C acclimated carp, BRL(37344) induced significant increases in fH and Q whereas Sv was significantly decreased. While temperature did not affect the change (increase vs. decrease) in cardiac variables, the magnitude and on-set of responses differed. For instance, fH, Sv and Q responded significantly faster to ß₃-AR stimulation in 23 °C carp. In contrast, maximum responses of fH and Q were significantly higher in 23 °C carp whereas the maximum response of Sv was significantly greater in 12 °C carp. These findings suggest that temperature acclimation induced changes in β₃-AR receptor functionality (e.g. density and/or affinity). Stimulation of β₃-ARs in 23 °C acclimated channel catfish (Ictalurus punctatus) caused significant increases in fH, Sv and Q. The increase in Sv was opposite to the decrease observed in 23 °C acclimated common carp. SR(59230A) induced significant decreases in Sv and Q but had no effect in carp (23 °C). Results suggest species diversity in the density and affinity or structure of ß₃-ARs which may explain the different cardiac responses to ß₃-AR ligands. PMID:25882086

  10. Production of Fish Hydrolysates Protein from Waste of Fish Carp (Cyprinus Carpio) By Enzymatic Hydrolysis

    OpenAIRE

    Dede Saputra; Tati Nurhayati

    2016-01-01

    Fish Protein Hydrolysates (FPH) is the mixed products of polypeptide, dipeptides, and amino acid. It can be produced from materials that contained of protein by acid reaction, base reaction or enzymatic hydrolysis. The objectives of this study were to study the production of FPH from fish carp meat at post rigor phase and viscera by enzymatic hydrolysis, to determine the specific activity of papain enzyme, and to determine the solubility of FPH. Capacity of fish hydrolyzing can be...

  11. Khawia japonensis (Cestoda: Caryophyllidea): another invasive parasite of carp, Cyprinus carpio L., imported to Europe

    Czech Academy of Sciences Publication Activity Database

    Scholz, Tomáš; Binh, T. T.; dezfuli, B. S.

    2011-01-01

    Roč. 34, č. 12 (2011), 943-949. ISSN 0140-7775 R&D Projects: GA ČR GA524/08/0885; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : carp * fish movements * identification key * man-assisted introduction * tapeworms Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.000, year: 2011

  12. INDUCED GYNOGENESIS OF COMMON CARP (Cyprinus carpio) AND GRASSCARP (Ctenopharyngodon idella) BY UV-LAMP IRRADIATION

    OpenAIRE

    Andrea Kolak; Yu Lou; Tomislav Treer; Ivica Aničić; Roman Safner

    1996-01-01

    In this paper the short review of fish gynogenesis, as one of the importani fields of research in ichtiogenetics, is presented. Two methods of own research are described. One of them is mitotic gynogenesis of common carp by 1,1 τ0 Another one is meiotic gynogenesis of grasscarp by 0,2-0,3 τ0. The scientific importance of gynogenetic researches is stated, but also the value of classical genetic methods, which are still more important in practical work.

  13. MOST FREQUENT CARP (Cyprinus carpio L.) DISEASES IN PONDS FROM 1994. TO 1998

    OpenAIRE

    Zdravko Petrinec; Milan Božić; A Berc; Ante Jelić

    1999-01-01

    Fish health monitoring was carried out in the period from 1994. to 1998. for 252 fresh water ponds. Findings about health status depended upon the quality of health control measures such as ichthyotechnic, ichthyosanitary and ichthyohygieni methods. Most frequently diagnosed diseases and their percentages of incidence were: carp erythrodermatytis in 5,82 %, ichthyophtiriasis in 13,51 %, trihodiniasis in 33,568 %, dactylogirosis and gyrodactilosis in 35,75 %, botriocephalosis in 14,99 % and sw...

  14. GUSTATORY RESPONSE OF COMMON CARP CYPRINUS CARPIO TO VARIABLE CONCENTRATIONS OF TWO STIMULATORY AMINO ACIDS

    Directory of Open Access Journals (Sweden)

    Jonathan David Wood

    2013-04-01

    Full Text Available Common carp possesses a highly evolved gustatory system that is stimulated by a narrow range of free amino acids including L-cysteine and L-proline. A synergetic effect on the gustatory response of a combination of these two substances has not previously been demonstrated. In this study, groups of common carp were randomly presented with different concentrations of L-cysteine (0-0.1 M and L-proline (0-0.05 M in agar pellets in one minute trials. First retention duration, total retention duration during each trial and the number of ingestions (pellet acceptances were recorded for each of a total of 690 trials. Palatability, pellet consumption rate and average pellet acceptances were calculated for each pellet type. It was shown that L-cysteine was more highly stimulatory than L-proline but no synergism between the two regarding the gustatory response was observed. The results are relevant for the formulation of aquafeeds and angling baits for carp.

  15. FACTORS AFFECTING TRANSMISSION AND SPREAD OF THE VIRUS SPRING VIREMIA OF CARP (CYPRINUS CARPIO)

    OpenAIRE

    N. Kharkavlyuk; N. Matvienko; О. Kovbasiuk

    2014-01-01

    Purpose. Spring viremia of carp (SVC) is a viral disease of cyprinids, the causative agent of which is a RNA-containing virus. The virus is represented by one serotype. This disease was firstly described in Yugoslavia by N.Fijan (1968), in Russia – by N.N. Rudykov (1971). The virus has similar morphology as viruses, which are causative agents of a number of salmonid diseases (VHS, IHN), differing from them by cultural properties. Avirulent strains are among field isolates. Outbreaks of spring...

  16. Early ontogeny of common carp (Cyprinus carpio) at low concentrations of the organic solvent DMSO

    Czech Academy of Sciences Publication Activity Database

    Máchová, J.; Prokeš, Miroslav; Kroupová, H.; Svobodová, Z.; Mácová, S.; Doleželová, P.

    Vodňany : University of South Bohemia in České Budějovice, Research Institute of Fish Culture and Hydrobiology, 2008 - (Velíšek, J.; Kroupová, H.). s. 77 [International workshop on the aquatic toxicology and biomonitoring /1./. 27.08.2008-29.08.2008, Vodňany] Institutional research plan: CEZ:AV0Z60930519 Keywords : carp * dimethyl sulfoxide Subject RIV: EH - Ecology, Behaviour http://www.vurh.jcu.cz/images/stories/1st_workshop/abstract_book.pdf

  17. Effect of potassium permanganate, cobalt chloride and vitamin B complex on the haematological parameters of cadmium treated common carp, Cyprinus carpio.

    Science.gov (United States)

    Kaviraj, A; Das, B K

    1995-01-01

    Effect of potassium permanganate, cobalt chloride and vitamin B complex on the haematological parameters of common carp Cyprinus carpio were markedly influenced by a treatment of 2.5 mg/l Cd in the laboratory for 96 hours. Hb% and ESR values were increased but the RBC and PCV values were reduced. Treatment of KMnO4 (1 mg/l) or CoCl2 (2 mg/l) induced a further reduction of RBC of the Cd treated fish. But parameters like PCV, MCV and MCH of Cd treated fish were not affected by the treatment of KMnO4 and CoCl2. Intramuscular injection of vitamin B complex did not produce any impact on ESR and MCV values of the Cd treated exposed fish but most of the other parameters of such fish were found comparable to control indicating that vitamin B complex could counteract Cd to reduce its ill effect. PMID:8580524

  18. Effect of heavy metals on the level of vitamin E, total lipid and glycogen reserves in the liver of common carp (Cyprinus carpio L.

    Directory of Open Access Journals (Sweden)

    Vinodhini Rajamanickam

    2008-06-01

    Full Text Available The aim of this study is to examine some changes in the biochemical profile of the liver tissue of common carp (Cyprinus carpio L. exposed to a sublethal concentration of heavy metal mixture (cadmium, chromium, nickel and lead. The biochemical profile, specifically glycogen, total lipid and vitamin E content in the liver tissue was examined and compared to that of the control group. The exposed group showed a marked decline in glycogen and vitamin E reserves. Conversely an increase in total lipid in comparison to control was observed. The result reflects the sensitivity of these biochemical parameters to the effects of sublethal levels of combined heavy metals for this the widely consumed freshwater fish.

  19. Organic Chemical Concentrations and Reproductive Biomarkers in Common Carp (Cyprinus carpio) Collected from Two Areas in Lake Mead, Nevada, May 1999-May 2000

    Science.gov (United States)

    Goodbred, Steven L.; Leiker, Thomas J.; Patiño, Reynaldo; Jenkins, Jill A.; Denslow, Nancy D.; Orsak, Erik; Rosen, Michael R.

    2007-01-01

    The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, National Park Service, Bureau of Reclamation, and Nevada Department of Wildlife, collected and assessed data to determine the general health and reproductive status of common carp (Cyprinus carpio) at two study areas in Lake Mead, Nevada, during May 1999-May 2000. These data will form the basis of interpretations and provide a comparison for continuing studies on the health of the ecosystem in Lake Mead. One study area, Las Vegas Bay, is in the western part of Lake Mead. Las Vegas Bay receives inflows from Las Vegas Wash, which is predominantly tertiary-treated wastewater effluent, and to a lesser extent stormwater runoff from Las Vegas, Henderson, and other nearby communities, and from ground water underlying Las Vegas Valley. The other study area, Overton Arm, is in the northern extent of Lake Mead. Overton Arm receives inflow from the Virgin and Muddy Rivers, which historically are not influenced by wastewater effluent. Both sexes of common carp were collected bimonthly for 12 months using boat-mounted electrofishing gear (a direct electric current is used to temporarily immobilize fish for capture) to determine their health and reproductive status and any relation between these factors and environmental contaminants. This report presents fish tissue chemistry, organic chemical compound concentrations, and biomarker data for 83 male common carp collected from Las Vegas Bay, similar organic chemistry results for 15 male common carp, and similar biomarker measures for 80 male common carp collected from Overton Arm. Tissue chemistry results also are presented for 16 female common carp and biomarker measures for 79 female common carp collected from Las Vegas Bay, and tissue chemistry results for 15 female common carp and biomarker measures for 81 female common carp collected from Overton Arm. Thirty-three organic chemical compounds plus total concentrations for four groups of

  20. Beta-glucan bath promote wound healing in common carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Przybylska, Dominika Alicja; Schmidt, Jacob; Nielsen, Michael Engelbrecht

    β-glucans are well known for their ability to modulate the immune system. These polysaccharides, derived from fungi, plants and bacteria cell wall [1] potently trigger inflammatory response in infected host [2]. The effects of β-glucans depend on the origins, route of administration, molecular...... healing process [1, 4]. Previous studies have shown that β-glucans stimulate production of pro-inflammatory mediators, cytokines and chemokines like e.g. IL-8, IL-1b, or IL-6 [5]. Studies in higher vertebrates clearly show that both PAMPs (pathogen associated molecular pattern) and DAMPs (danger......-associated molecular pattern) cause inflammation. The aim of this study was to investigate capability to modulate immune parameters during the wound healing processes of two commercially available β–glucans. In in vivo study, carps of ~50g were anaesthetised and wounded with 5mm biopsy punches. During the extent of...

  1. Βeta-glucans promote wound healing in common carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Przybylska, Dominika Alicja; Schmidt, Jacob; Nielsen, Michael Engelbrecht

    β-glucans are well known for their ability to modulate the immune system. These polysaccharides, derived from fungi, plants and bacteria cell wall [1] potently trigger inflammatory response in infected host [2]. The effects of β-glucans depend on the origins, route of administration, molecular...... healing process [1, 4]. Previous studies have shown that β-glucans stimulate production of pro-inflammatory mediators, cytokines and chemokines like e.g. IL-8, IL-1b, or IL-6 [5]. Studies in higher vertebrates clearly show that both PAMPs (pathogen associated molecular pattern) and DAMPs (danger......-associated molecular pattern) cause inflammation. The aim of this study was to investigate capability to modulate immune parameters during the wound healing processes of two commercially available β–glucans. In in vivo study, carps of ~50g were anaesthetised and wounded with 5mm biopsy punches. During the extent of...

  2. Hepatoprotective effects of a Chinese herbal formulation, Yingchen decoction, on olaquindox-induced hepatopancreas injury in Jian carp (Cyprinus carpio var. Jian).

    Science.gov (United States)

    Zhou, Jishu; Li, Chao; Wang, Lihong; Ji, Hong; Zhu, Tianhe

    2015-02-01

    In order to identify effective hepatoprotective herbs for clinical application in fish farming, 200 mg/kg olaquindox (OLA) was added to a basal diet (group 1, control) to form OLA diet (group 2), then 1.35, 2.7 and 5.4 % (w/w) of a Chinese herbal formulation, Yingchen decoction (YCD), were added to the OLA diet to form three additional diets for groups 3, 4 and 5, respectively. A total of 375 juvenile Jian carp (Cyprinus carpio var. Jian) (52.12 ± 2.95 g/tail) were divided into five groups (triplicates per group) and fed the five diets mentioned above, respectively, for 6 weeks. At the termination of feeding experiment, serum biochemical indexes, viability of hepatocytes and the hepatopancreas microstructure for each group were detected and observed. The results showed that serum ALT and AST in group 2 were significantly higher than the control (P < 0.05). Plasma membranes hepatocyte nuclei in group 2 were found to be mostly indistinct, compared to group 1, and gradually recovered with the increasing supplementation of YCD in group 3, 4 and 5. The viability of isolated hepatocytes in group 2 was the lowest and gradually recovered with the increasing supplementation of YCD in group 3, 4 and 5. The results suggest that YCD protected the Jian carp hepatopancreas against injury from OLA, and that 5.4 % YCD would be the optimum dosage in a Jian carp diet. PMID:25477196

  3. Effects of incorporating in diets cold-pressed rapeseed cake on the growth performance, nutrient utilization, and body composition of common carp (Cyprinus carpio L.

    Directory of Open Access Journals (Sweden)

    Mazurkiewicz Jan

    2015-06-01

    Full Text Available Alternative proteins from vegetal sources are being studied, because of the high costs and limited resources of fish meal. The aim of this study was to determine the possibility of including cold-pressed rape cake (CPRC as a partial protein substitute in diets for common carp, Cyprinus carpio L. Common carp fry were stocked into experimental ponds at a density of 30 fish per pond. The effects on growth, feeding efficiency, and fish body composition were studied for four amounts of CPRC (0, 70, 130, 200 g × kg-1. Statistically significant higher final weights (528-530 g were obtained with fish fed diets with 130 and 200 g × kg-1 CPRC. The fish growth rate was nearly identical in all variants (SGR of 3.3-3.4 % d-1. Similar results were presented in FCR at 1.3, and in PER at 2.2. Our results suggest that it is possible to include up to 200 g × kg-1 of CPRC in diets for two-year old common carp without significant effects on growth, nutritive efficiency, or the proximate composition of the fish.

  4. Effect of sub-acute exposure to TiO2 nanoparticles on oxidative stress and histopathological changes in Juvenile Carp (Cyprinus carpio)

    Institute of Scientific and Technical Information of China (English)

    HAO Linhua; WANG Zhenyu; XING Baoshan

    2009-01-01

    Increasing application of nanotechnology highlights the need to clarify and understand nanotoxicity. Mammalian and in vitro studies have raised concerns about the toxicity of titanium dioxide nanoparticles (TiO2 NPs), but there are limited data on ecotoxicity to aquatic organisms. In this work, the objective was to assess the sub-acute toxicity of TiO2 NPs to carp (Cyprinus carpio). Superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities and lipid peroxidation (LPO) levels in liver, gill and brain tissues of carps varied with concentration of TiO2 NPs suspensions and exposure time (up to 8 d). As a result, 100 and 200 mg/L TiO2 NPs caused statistically significant decrease in SOD, CAT and POD activities and significant increase in LPO levels in tissues (P < 0.05), suggesting that the fish exposed to these two concentrations of TiO2 NPs suffered from the oxidative stress. The extent of depletion of antioxidant enzymes activities and the elevation of LPO in the liver was the greatest, indicating that the liver might be the most susceptible organ to TiO2 NPs exposure. In addition, carps had gill pathologies including edema and thickening of gill lamellae as well as gill filaments, and liver pathologies including necrotic and apoptosis hepatocytes after exposed to 100 and 200 mg/L TiO2 NPs for 20 d. These results indicate a potential risk from TiO2 NPs released into the aqueous environment.

  5. Identification of methyl triclosan and halogenated analogues in male common carp (Cyprinus carpio) from Las Vegas Bay and semipermeable membrane devices from Las Vegas Wash, Nevada

    Science.gov (United States)

    Leiker, T.J.; Abney, S.R.; Goodbred, S.L.; Rosen, Michael R.

    2009-01-01

    Methyl triclosan and four halogenated analogues have been identified in extracts of individual whole-body male carp (Cyprinus carpio) tissue that were collected from Las Vegas Bay, Nevada, and Semipermeable Membrane Devices (SPMD) that were deployed in Las Vegas Wash, Nevada. Methyl triclosan is believed to be the microbially methylated product of the antibacterial agent triclosan (2, 4, 4'-trichloro-4-hydroxydiphenyl ether, Chemical Abstract Service Registry Number 3380-34-5, Irgasan DP300). The presence of methyl triclosan and four halogenated analogues was confirmed in SPMD extracts by comparing low- and high-resolution mass spectral data and Kovats retention indices of methyl triclosan with commercially obtained triclosan that was derivatized to the methyl ether with ethereal diazomethane. The four halogenated analogues of methyl triclosan detected in both whole-body tissue and SPMD extracts were tentatively identified by high resolution mass spectrometry. Methyl triclosan was detected in all 29 male common carp from Las Vegas Bay with a mean concentration of 596????g kg- 1 wet weight (ww) which is more than an order of magnitude higher than previously reported concentrations in the literature. The halogenated analogs were detected less frequently (21%-76%) and at much lower concentrations (< 51????g kg- 1 ww). None of these compounds were detected in common carp from a Lake Mead reference site in Overton Arm, Nevada.

  6. DNA vaccine protects ornamental koi (Cyprinus carpio koi) against North American spring viremia of carp virus

    Science.gov (United States)

    Emmenegger, E.J.; Kurath, G.

    2008-01-01

    The emergence of spring viremia of carp virus (SVCV) in the United States constitutes a potentially serious alien pathogen threat to susceptible fish stocks in North America. A DNA vaccine with an SVCV glycoprotein (G) gene from a North American isolate was constructed. In order to test the vaccine a challenge model utilizing a specific pathogen-free domestic koi stock and a cold water stress treatment was also developed. We have conducted four trial studies demonstrating that the pSGnc DNA vaccine provided protection in vaccinated fish against challenge at low, moderate, and high virus doses of the homologous virus. The protection was significant (p DNA immunized fish were challenged 28-days post-vaccination (546 degree-days) and experienced low mortalities varying from 10 to 50% with relative percent survivals ranging from 50 to 88%. The non-vaccinated controls and mock construct vaccinated fish encountered high cumulative percent mortalities ranging from 70 to 100%. This is the first report of a SVCV DNA vaccine being tested successfully in koi. These experiments prove that the SVCV DNA (pSGnc) vaccine can elicit specific reproducible protection and validates its potential use as a prophylactic vaccine in koi and other vulnerable North American fish stocks.

  7. Dietary microbial levan enhances cellular non-specific immunity and survival of common carp (Cyprinus carpio) juveniles.

    Science.gov (United States)

    Rairakhwada, Dina; Pal, A K; Bhathena, Z P; Sahu, N P; Jha, A; Mukherjee, S C

    2007-05-01

    A preliminary study with a 75days feeding trial was conducted to study the immunomodulatory effect of microbial levan on Cyprinus carpio juveniles. Five purified isonitrogenous and isocaloric diets with graded levels of levan, namely (T(1)) 0.1% levan, (T(2)) 0.2% levan, (T(3)) 0.5% levan, (T(4)) 1.0% levan, and a control group without levan were fed to five groups of fishes in triplicate. The total erythrocyte count and haemoglobin content was significantly (p0.05) when compared with the control group. The respiratory burst activity (NBT) of blood phagocytes and lysozyme activity was also highest in T(3) group. The relative survival percentage after challenge with Aeromonas hydrophila was highest (100%) in the T(3) group followed by 83.33% and 66.67% in the T(2) and T(4) groups, respectively. This suggests that microbial levan at 0.5% can be used as a dietary immunostimulant for C. carpio juveniles. This is the first report on microbial levan having an immunomodulatory effect on C. carpio. PMID:17158064

  8. The effects of ciprofloxacin on early life stages of common carp (Cyprinus carpio).

    Science.gov (United States)

    Zivna, Dana; Plhalova, Lucie; Chromcova, Lucie; Blahova, Jana; Prokes, Miroslav; Skoric, Misa; Marsalek, Petr; Praskova, Eva; Stepanova, Stanislava; Svobodova, Zdenka

    2016-07-01

    The authors performed a toxicity test with ciprofloxacin in fertilized eggs of common carp according to guideline 210 of the Organisation for Economic Co-operation and Development. The tested concentrations were 1 μg L(-1) , 100 μg L(-1) , 500 μg L(-1) , 1000 μg L(-1) , and 3000 μg L(-1) . Accelerated hatching was found in all groups exposed to ciprofloxacin, but significant growth reduction was found only in the group exposed to the highest concentration (3000 μg L(-1) ). Increased numbers of macroscopic morphological anomalies were observed on day 6 of the test (after hatching). The highest numbers of macroscopic morphological anomalies were observed in the groups of free embryos and larvae exposed to ciprofloxacin concentrations of 100 μg L(-1) , 500 μg L(-1) , 1000 μg L(-1) , and 3000 μg L(-1) (20-23% of tested samples). A gradual decrease in glutathione S-transferase activity was detected in all experimental groups exposed to ciprofloxacin, but significant differences (p ciprofloxacin concentrations of 500 μg L(-1) and 3000 μg L(-1) (p ciprofloxacin. The lowest-observed-effect concentration of ciprofloxacin was 1 μg L(-1) . These results suggest that hatching, early ontogeny, occurrence of morphological anomalies, antioxidant and biotransformation enzyme activity, and lipid peroxidation in fish can be affected by ciprofloxacin. Environ Toxicol Chem 2016;35:1733-1740. © 2015 SETAC. PMID:26632160

  9. Effect of organic manure and inorganic fertilizer on the growth and proximate composition of common carp, Cyprinus carpio.

    Science.gov (United States)

    Kour, Sandeep; Masud, Sahar; Khan, Asma

    2016-01-01

    The experiment was conducted to evaluate the effect of chicken manure and inorganic fertilizer (single super phosphate and urea) on growth performance viz. increase in wet body weight, length, specific growth rate (SGR), condition factor (CF), survival rate and proximate composition of Cyprinus carpio. Experimental water in tubs was treated in duplicates with low (PT) @8000 kgha , medium (PT2) @10,000 kgha(-1) and high (PT) @12,000 kg/ha doses of chicken manure and also with low, medium and high dose of inorganic fertilizer(urea + SSP) @ 104 kgha(-1) + 155 kgha(-1) (IT(1)), @218 kgha(-1) + 310 kg/ha (IT2) and 322 kgha(-1) +470 kgha(-1)(IT3) respectively, along with control. At the end of the experiment, C. carpio showed maximum growth in PT, with significant higher SGR and CF. Survival rate ranged beween 93.3 to 100 % in all the treatments. Level of crude protein and total fat were a2so highest in PT, Moisture and carbohydrate showed no significant changes while ash content changed significantly in all the treatments and control. These observations indicate that chicken manure alone was effective to stimulate productivity and growth of fish without affecting proximate composition of fish meat. PMID:26930873

  10. Effects of biofloc on growth performance, digestive enzyme activities and liver histology of common carp (Cyprinus carpio L.) fingerlings in zero-water exchange system.

    Science.gov (United States)

    Najdegerami, Ebrahim H; Bakhshi, Farideh; Lakani, Forouzan Bagherzadeh

    2016-04-01

    Biofloc technology is considered as a method that degrades organic waste by microorganisms and produces microbial flocs. A 30-day experiment was performed to investigate the effects of partial replacement of daily feeding intake with biofloc on the growth performances, digestive enzymes activity and liver histology of the common carp Cyprinus carpio L. fingerlings. Two hundred and eight healthy fingerlings (58.6 ± 0.2 g) were randomly distributed in 12 tanks (30 L) at a density of 25.4 kg m(-3) and fed experimental treatments (100 % daily feeding rate as a control, biofloc + 75% daily feeding rate, biofloc + 50% daily feeding rate, biofloc + 25% daily feeding rate). At the end of experiment, the results indicated that the highest weight gain was observed in the fish fed BFT 75% and control which differed significantly from those fed BFT 25 % (P 0.05). No significant difference was observed in case of lipase, amylase and alkaline phosphatase activity between the treatments. In the liver, histological alterations were found in the treatments, and feeding the fish with BFT 75% significantly improved hepatocellular quantification and qualification than the other groups. The results obtained in this experiment suggest that the biofloc improves growth performances, digestive enzyme activity and liver condition of the common carp fingerlings when 25% of daily feeding rate (BFT 75%) was replaced with one carbohydrate such as molasses in zero-water exchange system. PMID:26530301

  11. The first appearance of Rodlet cells in carp (Cyprinus carpio L.) ontogeny and their possible roles during stress and parasite infection.

    Science.gov (United States)

    Mazon, A F; Huising, M O; Taverne-Thiele, A J; Bastiaans, J; Verburg-van Kemenade, B M L

    2007-01-01

    The origin and function of rodlet cells (RCs) are still a matter of discussion. Whereas the exogenous hypothesis considers them parasites, the endogenous hypothesis regards them as a genuine fish cell population with a secretory and/or leukocyte function. In order to shed more light on these questions we focused on the location and appearance of RCs during carp (Cyprinus carpio) ontogeny. Typical RCs were seen at 5days post fertilisation (dpf) between kidney and intestine, at 6dpf in the intestine and at 8dpf in both anterior and posterior kidney and in the abdominal cavity among the mesothelial cells. The RC number increased with age and after 14dpf they were also present in gills. The early appearance of the RCs during carp ontogeny support the endogenous hypothesis stating that RCs are genuine constituents of fish tissue and suggest that they are 'immune cells'. The fact that the RCs of the gills secrete their content into the surrounding water, combined with the strategic location around blood vessels in kidney and within intestinal epithelium, would also support an important role in host defense. To investigate whether RC numbers in gills and kidney are related to typical fluctuations in the physiology during stress and infection we counted their number in gills and kidney after parasite infection and stress. In the gills the number of RCs increased after infection but did not change after stress while in the kidney their number increased after stress and no significant changes were observed after infection. PMID:16843681

  12. Investigation of Van Gogh-like 2 mRNA regulation and localisation in response to nociception in the brain of adult common carp (Cyprinus carpio).

    Science.gov (United States)

    Reilly, Siobhan C; Kipar, Anja; Hughes, David J; Quinn, John P; Cossins, Andrew R; Sneddon, Lynne U

    2009-11-20

    The Van Gogh-like 2 (vangl2) gene is typically associated with planar cell polarity pathways, which is essential for correct orientation of epithelial cells during development. The encoded protein of this gene is a transmembrane protein and is highly conserved through evolution. Van Gogh-like 2 was selected for further study on the basis of consistent regulation after a nociceptive stimulus in adult common carp and rainbow trout in a microarray study. An in situ hybridisation was conducted in the brain of mature common carp (Cyprinus carpio), 1.5 and 3 h after a nociceptive stimulus comprising of an acetic acid injection to the lips of the fish and compared with a saline-injected control. The vangl2 gene was expressed in all brain regions, and particularly intensely in neurons of the telencephalon and in ependymal cells. In the cerebellum, a greater number (P=0.018) of Purkinje cells expressed vangl2 after nociception (n=7) compared with controls (n=5). This regulation opens the possibility that vangl2 is involved in nociceptive processing in the adult fish brain and may be a novel target for central nociception in vertebrates. PMID:19781599

  13. Toxicity Testing and the Effect of Landfill Leachate in Malaysia on Behavior of Common Carp (Cyprinus carpio L., 1758; Pisces, Cyprinidae

    Directory of Open Access Journals (Sweden)

    Jaffar Y.M. Alkassasbeh

    2009-01-01

    Full Text Available Problem Statement: Landfill leachate had been implicated in environmental pollution, developmental anomalies, birth defect and surface and groundwater pollution worldwide. This study has been conducted to determine the toxicity of landfill leachate from three different landfills in Malaysia on fry common carp (Cyprinus carpio L., 1758; Pisces, Cyprinidae in combination with some physico-chemical parameters analysis. Approach: Leachates were collected from Air Hitam (AHL, Ampar Tenang (ATL and Sungai Sedu (SSL landfills. The experiments were performed as three replicates using a total of 180 carps for each definitive test. The semi-static renewal method of acute toxicity test was used. The data obtained were statically evaluated by the use of the EPA computer program based on Finney’s Probit Analysis Method. Results: The leachate used in this study had an invariable of (1640-7600 mg L-1 COD and (321.22-956.86 mg L-1 Ammonical-N. The 96 h LC50 values of landfills leachate from the three landfills using fry C. carpio individuals with an average weight of 0.92±0.24 g and average length of 3.83±0.19 cm were found to be 1.132, 2.0 and 3.822% respectively. Among the behavioral changes observed for the individual fish at different leachate concentrations, decline in general activity, loss of balance, breathing difficulties, excessive mucosal secretion and gathering at the surface for breathing. Conclusion: This study of acute toxicity to the recommended fish species, C. caprio, is considered the first in Malaysia. Further research with toxicity testing methods directly on fish will be very useful in assessing possible ecological risk assessment of landfill leachate.

  14. Effects of sub lethal concentration of Chloramin T on growth, survival, haematocrit and some blood biochemical parameters in common carp fry (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Mohamad R. Imanpoor

    2011-07-01

    Full Text Available This study was done in Gorgan University of Agricultural Sciences and Natural Resources, in 2009, during 8 weeks to survey effects of different concentrations of Chloramin T on fry common carp (Cyprinus carpio (Linnaeus, 1758. According to the pre experiment data, lethal concentration, the lowest observed effect concentration, maximum allowable toxicant concentration and No Observed Effect Concentration of Chloramin T (Halamid in common carp (C. carpio fry were respectively 40.9, 27.1, 4.90 and 11.28 mg/L-1. Hence, the range of our experiment was between 0 to 25 mg L-1 which was divided to five treatments (0, 5, 10, 15 and 25 mg/L-1. At the end of experiment we calculated growth factor (special growth rate and food conversion ratio and stress indices (glucose total protein and percent of haematocrit and then they were compared with the controlled group. Our study results showed no significant difference between percentage of increase in body weight (280.4±25.79 - 200.4±10.16, special growth rate (2.34±0.25 - 1.96±0.06 and food conversion ratio (0.84±0.05 - 0.60±0.12 in experimental lots of fishes. There is also no significant difference between glucose (146.82±0.79 - 99.54±1.89 and total protein (3.61±0.45 - 2.82±0.06 in experimental groups (P>.05. However there is a significant difference between the percentages of haematocrit (57.34±4.99 - 40.74±2.17 and cholesterol (362.05±24.38 - 134.92±17.59 in these groups (P<0.05.

  15. Potential for bias in using hybrids between common carp (Cyprinus carpio) and goldfish (Carassius auratus) in endocrine studies: a first report of hybrids in Lake Mead, Nevada, U.S.A

    Science.gov (United States)

    Goodbred, Steven L.; Patino, Reynaldo; Orsak, Erik; Sharma, Prakash; Ruessler, Shane

    2013-01-01

    During a 2008 study to assess endocrine and reproductive health of common carp (Cyprinus carpio) in Lake Mead, Nevada (U.S.A.) we identified two fish, one male and one female, as hybrids with goldfish (Carassius auratus) based on morphology, lateral line scale count, and lack of anterior barbels. Gross examination of the female hybrid ovaries indicated presence of vitellogenic ovarian follicles; whereas histological evaluation of the male hybrid testes showed lobule-like structures with open lumens but without germ cells, suggesting it was sterile. Because common carp/goldfish hybrids are more susceptible to gonadal tumors and may have different endocrine profiles than common carp, researchers using common carp as a model for endocrine/reproductive studies should be aware of the possible presence of hybrids.

  16. Obtención, caracterización microbiológica y físico-química de ensilado biológico de carpa (Cyprinus carpio) - Obtaining, characterization microbiological and physic-chemical of carp biological silage (Cyprinus carpio

    OpenAIRE

    Fernández Herrero, Adriana L.; Manca, Emilio; Tabera, Anahí; Agüeria, Daniela; Sanzano, Pablo; Grosman, Fabián

    2011-01-01

    ResumenEl propósito del presente estudio fue determinar los cambios en la calidad nutricional y composición química que ocurren durante el ensilado de desechos de carpa (Cyprinus carpio), y determinar cual de las dos proporciones de miel: yogur es la apropiada para su utilización como fuente proteica en la alimentación animal.SummaryThe purpose of the present study was to determine the changes in the nutritional quality and the chemical composition that occur during the ensilage of waste carp...

  17. Effet of Mercuric Chloride and Cadmium Chloride on Gonadal Function and Its regulation in Sexually Mature Common Carp Cyprinus carpio

    Institute of Scientific and Technical Information of China (English)

    DilipMUKHERJEE; VinodKumar; 等

    1994-01-01

    Gnadal function in fish,Cyprinus carpio was significantly affected by sublethal doses of mecuric chloride(HgCl2)and cadmium chloride(CdCl2)in chronic(45days)exposure,Parameters investigated were nonesterified(NE)and esterified(E)cholesterol of ovary, liver and serum and ovarian 3β-Hydroxysteroid and 17β-Hydroxysteroid dehydrogenase enzyme activity and servum and pituitary gonadotropin(GtH)levels.Both the pollutats were able to reduce the hypothalamic extract(HE)or gonadotropin releasing hormone (GnRH)induced pituiteray GtH release in vitro.Short term(96h)exposure of the fish to the polltants had no significant effect on the gonadal founction.In addition to the deleterious effect of pollutants on the gonadal steroidogenesis and pituitary gonadotropin release,using [4-14C] cholesterol as a tracer it was found that for 45 days exposure,HgCl2 had an adverse effect on the transport of cholesterol from circulation to ovary.

  18. Use of the Integrated Biomarker Response to Measure the Effect of Short-term Exposure to Dibenz[a,h]anthracene in Common carp (Cyprinus carpio).

    Science.gov (United States)

    Kim, Ja-Hyun; Kim, Woo-Keun

    2016-04-01

    Dibenz[a,h]anthracene (DbA) is a polycyclic aromatic hydrocarbon that is released into the environment through incomplete combustion of gasoline, cigarettes, and coal tar. The effects of short-term (10 days) exposure of common carp (Cyprinus carpio) to DbA (0-50 µg L(-1)) were evaluated using the following four biomarkers: DNA damage, 7-ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, and vitellogenin (VTG) levels. An integrated biomarker response (IBR) was calculated for exposure to DbA, and the results were compared with those in our previous study of two other PAHs, benzo[k]fluoranthene (BkF) and benzo[a]pyrene (BaP). DbA exposure resulted in a significant (p IBR increased as the concentration of DbA increased. Based on the IBR values, the order of toxicity for the PAHs was BkF > BaP > DbA. Our results suggest that the IBR can be used as a quantitative tool for evaluating the responses of multiple biomarkers to PAH exposure. PMID:26744022

  19. Morphological and molecular characterization of Thelohanellus macrovacuolaris n. sp. (Myxosporea: Bivalvulida) infecting the palate in the mouth of common carp Cyprinus carpio L. in China.

    Science.gov (United States)

    Liu, Yang; Zhai, Yanhua; Gu, Zemao

    2016-08-01

    Thelohanellus macrovacuolaris n. sp. is described during a survey on myxozoan diversity of common carp Cyprinus carpio L. in China. It is characterized by the presence of round or ellipsoidal plasmodium in the palate in the mouth of host. Mature spores were pyriform in frontal view, lemon shaped in lateral view, measuring 21.6±0.9 (19.3-23.8) long, 12.5±0.7 (10.3-13.6) wide, and 10.2±0.4 (9.8-11.8) thick. Most spores were surrounded by the membrane sheath. Single polar capsule was round with an apophysis at its top end presented close to apex of spore, measuring 9.1±0.6 (8.0-10.0) in length, 8.6±0.5 (7.8-9.6) in width. Polar filaments coiled, with 7 to 8 turns. A large, round iodinophilous vacuole was present, with 5.8-7.5 in diameter. The present species is morphologically distinct from all other Thelohanellus species. The BLAST search indicated that the newly obtained small subunit ribosomal RNA (ssrRNA) gene sequence of T. macrovacuolaris n. sp. did not match any available sequences in GenBank. Phylogenetically, T. macrovacuolaris n. sp. was placed sister to Thelohanellus wangi in the Thelohanellus clade. Both morphology and ssrRNA gene sequence data revealed that the present parasite is a new species of genus Thelohanellus. PMID:26939982

  20. Effects of Pro-Tex on zebrafish (Danio rerio) larvae, adult common carp (Cyprinus carpio) and adult yellowtail kingfish (Seriola lalandi).

    Science.gov (United States)

    Boerrigter, Jeroen G J; van de Vis, Hans W; van den Bos, Ruud; Abbink, Wout; Spanings, Tom; Zethof, Jan; Martinez, Laura Louzao; van Andel, Wouter F M; Lopez-Luna, Javier; Flik, Gert

    2014-08-01

    Aquaculture practices bring several stressful events to fish. Stressors not only activate the hypothalamus-pituitary-interrenal-axis, but also evoke cellular stress responses. Up-regulation of heat shock proteins (HSPs) is among the best studied mechanisms of the cellular stress response. An extract of the prickly pear cactus (Opuntia ficus indica), Pro-Tex, a soluble variant of TEX-OE(®), may induce expression of HSPs and reduce negative effects of cellular stress. Pro-Tex therefore is used to ameliorate conditions during stressful aquaculture-related practices. We tested Pro-Tex in zebrafish (Danio rerio), common carp (Cyprinus carpio L.) and yellowtail kingfish (Seriola lalandi) exposed to aquaculture-relevant stressors (thermal stress, net confinement, transport) and assessed its effects on stress physiology. Heat shock produced a mild increase in hsp70 mRNA expression in 5-day-old zebrafish larvae. Pro-Tex increased basal hsp70 mRNA expression, but decreased heat-shock-induced expression of hsp70 mRNA. In carp, Pro-Tex increased plasma cortisol and glucose levels, while it did not affect the mild stress response (increased plasma cortisol and glucose) to net confinement. In gills, and proximal and distal intestine, stress increased hsp70 mRNA expression; in the distal intestine, an additive enhancement of hsp70 mRNA expression by Pro-Tex was seen under stress. In yellowtail kingfish, Pro-Tex reduced the negative physiological effects of transport more efficiently than when fish were sedated with AQUI-S(®). Overall, our data indicate that Pro-Tex has protective effects under high levels of stress only. As Pro-Tex has potential for use in aquaculture, its functioning and impact on health and welfare of fish should be further studied. PMID:24493298

  1. Effects of vitamins E and Riboflavin (B2 and combinations of them on the hematological parameters of common carp, Cyprinus carpio L., fingerlings

    Directory of Open Access Journals (Sweden)

    Sharifzadeh Seyedeh Atefeh

    2015-06-01

    Full Text Available In the present study, the effects of vitamins E, B2 and combinations of them on hematological parameters of common carp, Cyprinus carpio L., were investigated during a 56-day experiment. The various dietary levels of vitamins E, B2 and their combination were used as experimental treatments, as follows: T1: vitamin E (80 mg, T2: vitamin E (160 mg kg diet-1, T3: vitamin E (240 mg kg diet-1, T4: vitamin B2 (7 mg kg diet-1, T5: vitamin B2 (15 mg kg diet-1, T6: vitamin B2 (20 mg kg diet-1, T7: vitamin E (80 mg kg diet-1 + vitamin B2 (7 mg kg diet-1, T8: vitamin E (160 mg kg diet-1 + vitamin B2 (15 mg kg diet-1, T9: vitamin E (240 mg kg diet-1 + vitamin B2 (20 mg kg diet-1. One group not given vitamin supplements was the control. The values of red blood cells (RBC, hemoglobin (Hb, and hematocrit (Hct were higher in T7 than in the other experimental treatments. The values of white blood cells (WBC were higher in T2 than in the other treatments. The concentration of immunoglobulin (IgM was also lower in T10 than in the other experimental treatments. The highest values of mean corpuscular hemoglobin (MCH and mean corpuscular volume (MCV were observed in T2. No significant differences were noted among experimental groups in the values of mean corpuscular hemoglobin concentration (MCHC. This study showed that vitamin E and B2 supplements alter the hematological parameters of common carp.

  2. Some Population Parameters of Mirror Carp (Cyprinus carpio L., 1758 Inhabiting Atatürk Dam Lake

    Directory of Open Access Journals (Sweden)

    Songül YÜCE

    2016-04-01

    Full Text Available This study was conducted at Atatürk Dam Lake between April 2011-March 2012. In this study, a total of 231 (106 female, 125 male Cyprinus carpio L., 1758 samples were analyzed. It was determined that samples were ranged between I-X age groups and male/female ratio was determined as 1.18:1 Total length values were ranged between 13.5-59.2 cm for males, 14.6-59.3 cm for females; weight values were ranged between 53.2-4662.5 g for males, 56.4-4668.0 g for females; condition factor values were ranged between 1.182-4.175 for males and 1.251-3.514 for females. Length-weight relationship for males W=0.0346*TL2.807 (R2=0.95, female W=0.0246*TL2.8922 (R2=0.96 and in general population W=0.0295*TL2. 8472 (R2=0.95 were detected. VBBD (von Bertalanffy growth equation for all population parameters L∞=111.01, K=0.06, t0=-1.48, W∞=19746.57 and natural mortality (M is determined as 0.1671. The highest average Gonadosomatic index values were determined in April for both sexes. Egg diameter values were measured between 0.60-1.33 mm, and the highest average value was found in April (1.27 mm. Reproduction period was determined between April-July. Fecundity values was calculated between 13468-885762. The first maturation was determined to be in age group III (30.848 cm in total length for males and in age group IV (32.099 cm in total length for females.

  3. Effect of modified atmosphere and vacuum packaging on selected chemical parameters of rainbow trout (Oncorhynchus mykiss and carp (Cyprinus carpio cuts freshness

    Directory of Open Access Journals (Sweden)

    Babić Jelena A.

    2014-01-01

    Full Text Available The purpose of food packing in modified atmosphere is to extend its sustainability by preventing both biochemical processes and growth of spoilage bacteria. Gases or their mixtures which are mostly used in the modified atmosphere food packing technology are carbon-dioxide (CO2, oxygen (O2 and nitrogen (N2. The aim of our research was to examine the influence of packaging in modified atmosphere and vacuum on the total volatile basic nitrogen (TVB-N content and pH in muscle of rainbow trout (Oncorhynchus mykiss and common carp (Cyprinus carpio, as well as to determine the most suitable gas mixtures for packing of these freshwater species. Three sample groups of trout and carp cuts were investigated. The first two groups were packaged in modified atmosphere with different gas ratios: 60%CO2+40%N2 (I group and 40%CO2+60%N2 (II group, whereas the samples from third, control group, (III group were vacuum packaged. During trials samples were stored in refrigerator at +3°C. Determination of TVB-N and pH was performed on 1st, 7th and 14th day of storage. The obtained results indicate that the investigated mixtures of gases and vacuum as well had a significant influence on the values of TVB-N in trout and carp cuts samples. The lowest increase in TVB-N was established in trout and carp cuts samples from the group I, whereas the highest increase was established in samples from group III. Statistical significant difference (p < 0,001 between the average values of TVB-N for trout (I group: 18,17 ± 0,93; II group: 20,90 ± 0,81 and III group: 36,18 ± 2,65 mg N/100 g and carp cuts (I group: 26,74 ± 1,48; II group: 30,02 ± 0,31 and III group: 35,10 ± 1,75 mg N/100 g was established on 14th day. The lowest pH value was established in samples packaged in modified atmosphere with 60% CO2 +40% N2 (I group. On 14th day of testing the obtained value was 6,15 ± 0,09 for trout and 5,94 ± 1,11 for carp samples. Increase in pH value in trout samples packed in

  4. Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China

    Science.gov (United States)

    Ye, Lingtong; Lu, Mingmiao; Quan, Keyan; Li, Wenxiang; Zou, Hong; Wu, Shangong; Wang, Jiangyong; Wang, Guitang

    2016-05-01

    The mass mortality of pond-reared scattered mirror carp, Cyprinus carpio, caused by Thelohanellus kitauei, occurred at fish farms in Sichuan Province, southwest China. Morphological and molecular analyses were supplemented with histological evaluation of infected tissues to better understand the route of infection and the pathological effects of T. kitauei on the fish host. The intestine of the diseased host was full of large cysts of the myxosporean. The cysts range from 2 cm to 3.6 cm in diameter. Histopathology indicated that T. kitaue i first invaded the submucosa of the host intestine and then moved into the mucosa layers with the development of their spores, finally entering into the enteric cavity of the hosts after the disruption of mucosa layers. The pyriform spores of T. kitauei were surrounded by the transparent spore sheath, measuring 25.98 μm±0.95 μm in body length, 8.72 μm±0.51 μm in body width, and 7.86 μm±0.26 μm in body thickness. The single polar capsule was pyriform, measuring 14.73 μm±0.92 μm in length and 6.82 μm±0.45 μm in width, with eight to 10 turns of filament coils winding inside. Phylogenetic analysis based on the 18S small-subunit ribosomal DNA sequences indicated that minimal genetic differences were present between T. kitauei samples from South Korea and from China. Close affinity was found between the genus Thelohanellus and Myxobolus. Additionally, two polar capsule nuclei were found at the anterior end of the single polar capsule in spores of T. kitauei stained with hematoxylin and eosin, which suggested the separation of the genus Thelohanellus from Myxobolus.

  5. Heritability estimates for growth-related traits using microsatellite parentage assignment in juvenile common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Vandeputte, M.; Kocour, Martin; Mauger, S.; Dupont-Nivet, M.; DeGuerry, D.; Gela, David; Vallod, D.; Linhart, Otomar; Chevassus, B.

    Chile : Puerto Varas, 2003. s. 119. [Genetics in Aquaculture /8./. 09.11.2003-15.11.2003, Puerto Varas] Institutional research plan: CEZ:AV0Z5045916 Keywords : common carp Subject RIV: ED - Physiology

  6. 鱼油在鲤饲料中的适宜用量%Optimum level of fish oil supplementation in carp (Cyprinus carpio) feed

    Institute of Scientific and Technical Information of China (English)

    任泽林; 郭庆; 霍启光; 卢建军

    2001-01-01

    在高蛋白质半纯化饲料中分别添加0、30、50、70、90 g/kg的未加抗氧化剂的新鲜鱼油,投喂58 g左右2龄鲤(Cyprinus carpio)鱼种46 d,结果表明:添加新鲜鱼油量为30 g/kg时,鲤生产性能最佳;鲤肝体比(HSI)、肝胰脏脂肪含量、肌肉营养不良症和肌肉渗出性损失随着鱼油添加量的增加而持续上升,而肌肉和肾脏氧化稳定性则随着鱼油添加量的增加而持续下降,当添加鱼油量升至30、70、70、50、70、70 g/kg时,上述6项指标与对照组差异显著(P<0.05)。综合各项指标,未添加抗氧化剂的新鲜鱼油在高蛋白质鲤饲料中适宜用量以不超过30 g/kg为宜。%Juvenile carp of 58 g were fed a high protein semi-purified feed supplemented with 0, 30, 50, 70, 90 g/kg fresh fish oil with no supplemention of antioxidant (Peroxide value,POV,0.64 mmol/kg ) for 46 days. The results showed that the carp had the best performance at 30 g/kg supplementation of fish oil. With the increment of fish oil supplementation, hepatopancreas somatic indices (HSI), fat content in hepatopancreas, muscular dystrophy and muscular exudative losses were increased, while oxidative stabilities of muscle and kidney were reduced. There were significant differences in fat content in hepatopancreas, muscular dystrophy, and oxidative stabilities of muscle and kidney between the control group (no fish oil supplementation) and the 70 g/kg or higher fish oil supplemented groups (P<0.05). HSI of the control group was significantly different with that at 30 g/kg or higher fish oil supplemented groups (P<0.05). Compared with the control group, the muscular exudative losses was significantly different at 50 g/kg or higher fish oil supplemented groups(P<0.05). In conclusion, fresh fish oil with no supplemention of antioxidant should be supplemented in carp feed with high protein at no more than 30 g/kg.

  7. β-glucan enriched bath directly stimulates the wound healing process in common carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Przybylska, Dominika Alicja; Schmidt, Jacob; Jiménez, Natalia Ivonne Vera;

    2013-01-01

    investigated. The aim of the present study was to investigate the biological impact of two different commercially available β glucan containing products on the wound healing process in carp. Throughout a two week experiment fish were kept either untreated (control), or in water supplemented with the two...

  8. An experimental study on the possibilities of direct transfer of caesium 137 from sediment to the carps (Cyprinus carpio L.)

    International Nuclear Information System (INIS)

    The exchanges of caesium 137 with the Rhone river water and deposits and its direct transfer from water and deposits to the carp are studied. The sediments have a high retention capacity for radiocaesium associated with fine particles and certain clays (illite). Caesium desorption from sediment to water is always very weak (less than 5% of the initial activity of the sediments); it varies with the agitation and salinity of the water. The transfer of caesium from the water to the carps is characterized by a regular increase of activity in the fish. After 63 days of experimentation, equilibrium has not been reached, the carp have retained only 1% of the water activity, and their concentration factor is about 4. The transfer of caesium from sediment to the fish is weak but regular. After 79 days of experimentation, equilibrium has not been reached. Less than 1% of the sediments activity is found in the fish; the transfer factor is about 10-3. Because of the high content of caesium 137 in sediments, the activity of the carp is increased and the concentration factor, calculated in relation to the surface water, is greater than 60

  9. Replacing moringa leaf (Moringa oleifera) partially by protein replacement in soybean meal of fancy carp (Cyprinus carpio)

    OpenAIRE

    Bundit Yuangsoi; Toshiro Masumoto

    2012-01-01

    Moringa oleifera Lam (Moringaceae) is a highly valued plant, distributed in many countries of the tropics and subtropics.The leaves are the protein source with an adequate profile of amino acids. The present study was undertaken in orderto determine the effect of a dietary of moringa leaves on digestibility and growth performance of fancy carp. Fish were fedwith diets containing isonitrogenouse and isoenergetic formulated by 20 and 50 g kg-1 of moringa leaves to replace protein insoybean. Fis...

  10. Molecular characterization and expression analysis of two new C-reactive protein genes from common carp (Cyprinus carpio)

    NARCIS (Netherlands)

    Falco, A.; Cartwright, J.R.; Wiegertjes, G.F.; Hoole, D.

    2012-01-01

    C-Reactive protein (CRP) plays an important role in the acute phase response. Transcripts encoding two new CRP-like molecules (ccCRP1 and ccCRP2) from European common carp have been characterized which has enabled seven CRP-like genes to be identified in zebrafish. 79.3% (ccCRP1) and 74.5% (ccCRP2)

  11. Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.)

    Institute of Scientific and Technical Information of China (English)

    Bo WU; Yong Hua SUN; Yan Wu WANG; Ya Ping WANG; Zuo Yan ZHU

    2005-01-01

    The integration pattern and adjacent host sequences of the inserted pMThGH-transgene in the F4 hGH-transgenic common carp were extensively studied. Here we show that each F4 transgenic fish contained about 200 copies of the pMThGH-transgene and the transgenes were integrated into the host genome generally with concatemers in a head-totail arrangement at 4-5 insertion sites. By using a method of plasmid rescue, four hundred copies of transgenes from two individuals of F4 transgenic fish, A and B, were recovered and clarified into 6 classes. All classes of recovered transgenes contained either complete or partial pMThGH sequences. The class Ⅰ, which comprised 83% and 84.5% respectively of the recovered transgene copies from fish A and B, had maintained the original configuration, indicating that most transgenes were faithfully inherited during the four generations of reproduction. The other five classes were different from the original configuration in both molecular weight and restriction map, indicating that a few transgenes had undergone mutation, rearrangement or deletion during integration and germline transmission. In the five types of aberrant transgenes, three flanking sequences of the host genome were analyzed. These sequences were common carp β-actin gene, common carp DNA sequences homologous to mouse phosphoglycerate kinase-1 and human epidermal keratin 14, respectively.

  12. PCR-cloning and gene expression studies in common carp (Cyprinus carpio) insulin-like growth factor-II.

    Science.gov (United States)

    Tse, Margaret C L; Vong, Queenie P; Cheng, Christopher H K; Chan, King Ming

    2002-05-01

    Insulin-like growth factor-II (IGF-II) is a member of a growth factor family related to fetal growth in mammals but its physiological role has not been clearly identified in fish. In teleosts, the basic mechanism of the growth hormone (GH)-IGF axis is known to be operative but in a different manner. For instance, IGF-I exhibits GH dependence whereas for IGF-II, its GH dependence varies in different fish species. In this study, we used polymerase chain reaction (PCR) to obtain a common carp IGF-II (ccIGF-II) cDNA fragment and methods of rapid amplification of cDNA ends (RACEs) to obtain a full-length ccIGF-II sequence. The ccIGF-II encodes for a predicted amino acid sequence showing identities of 70.6%, 68.7%, 63.4% and 35% in comparison with salmon, barramundi, tilapia and human IGF-II, respectively. The nucleotide identity between the open reading frame (ORF) of the ccIGF-II and ccIGF-I cDNA sequence is only 36.2%. Distribution of ccIGF-II mRNA levels in common carp tissues was also studied; ccIGF-II expressed in hepatopancreas, heart, and many other tissues in adult carps are similar to the levels of ccIGF-I except in gills and testis. ccIGF-II levels were significantly higher than that of ccIGF-I in most juvenile tissues except in hepatopancreas, where ccIGF-I was higher (threefold) than that of ccIGF-II. The levels of ccIGF-I were also higher than ccIGF-II in carp larvae, from pre-hatched stage to day 30 post-hatching. Injection of porcine GH (pGH) increased the IGF-I and IGF-II mRNA levels in the hepatopancreas and brain of juvenile carps. However, hepatic IGF-I mRNA levels were induced more than IGF-II by pGH, whereas ccIGF-II levels gave a higher response than IGF-I in the brain in response to GH induction. PMID:12020820

  13. Description of an as Yet Unclassified DNA Virus from Diseased Cyprinus carpio Species

    OpenAIRE

    Hutoran, Marina; Ronen, Ariel; Perelberg, Ayana; Ilouze, Maya; Dishon, Arnon; Bejerano, Izhak; Chen, Nissim; Kotler, Moshe

    2005-01-01

    Numerous deaths of koi and common carp (Cyprinus carpio) were observed on many farms throughout Israel, resulting in severe financial losses. The lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. Diseased fish exhibit fatigue and gasping movements in shallow water. Infected fish had interstitial nephritis and gill necrosis as well as petechial hemorrhages in the liver and other symptoms tha...

  14. Effect of Water Temperature on Feeding Rhythm in Common CarpCyprinus carpio haematopterus Temminck et Schlegel)

    Institute of Scientific and Technical Information of China (English)

    Chen Song-bo; Chen Wei-xing; Fan Zhao-ting

    2012-01-01

    Feeding rhythm of common carp was investigated from 4℃ to 34℃. The results indicated that there was a diel feeding rhythm for both adult (630-850 g) and youth (61-91 g) at all tested temperatures. There were two main activity peaks at 8:00-11:00 a.m. and 19:00-23:00 p.m., during which feeding quantities were 10.68%-32.53% and 16.25%-33.41% of the daily intake, respectively. When water temperature dropped to below 10℃, the feeding peak concentrated at 8:00-11:00 a.m. and 19:00 p.m. to 4:00 a.m. At 6℃, though both adult and youth would still feed, the feeding quantities were only 0.01% and 0.35% of body mass. Daily feeding rate of adult and youth reached 1.21% and 2.63% at 14℃, respectively. Both adult and youth carps reached the maximum daily feeding rate at 28℃, being 2.84% and 12.06% of body mass, respectively. The daily feeding rate of adult and youth reduced suddenly after at 34℃, and the daily feeding rate was only 0.74% and 9.45% of body mass, respectively. There was significant difference in daily feeding rate at different water temperatures (P〈0.05).

  15. Effects of Single and Joint Subacute Exposure of Copper and Cadmium on Heat Shock Proteins in Common Carp (Cyprinus carpio).

    Science.gov (United States)

    Jiang, Xuyang; Guan, Xueting; Yao, Linlin; Zhang, Hong; Jin, Xian; Han, Ying

    2016-02-01

    Copper (Cu) and cadmium (Cd) are the most common heavy metals that are easily detected in aquatic environments on a global scale. In this paper, we investigated the messenger RNA (mRNA) and protein levels of HSPs (HSP60, HSP70, and HSP90) in the liver of the common carp exposed to Cu, Cd, and a combination of both metals by real-time quantitative PCR and Western blot. The results indicated that in each exposure group, the mRNA levels of HSP60, HSP70, and HSP90 were increased significantly compared to the corresponding controls after 96 h of exposure (P challenges of stressful environments. PMID:26105544

  16. Genetic analysis of "all-fish" growth hormone gene trans- ferred carp (Cyprinus carpio L.) and its F1 generation

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Recombinant "all-fish" growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant "fast-growing" effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the F1 generation were genetically analyzed, which gave solid evidence for the hypothesis that 2-3 chromosomes are integrated with transgene. In addition, the distinct biological effects for multi- site-integrated transgenes were further discussed. The present study opens a door for the breeding of "fast-growing" transgenic fish.

  17. The Protective Effect of Liquorice Plant Extract on CCl4-Induced Hepatotoxicity in Common Carp (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Hassan Malekinejad

    2010-12-01

    Full Text Available The protective effect of liquorice plant extract (LPE on CCl4-induced hepatotoxicity in common carp was evaluated using fifty adult carps. The fish were cultured in a standard environment in terms of water flow rate, oxygen, pH, food and temperature. The fish were assigned into 5 groups (N = 10 as control, sham, and tests. The test groups were pre-treated for 3 h with various concentrations of LPE, 3 days before CCl4 exposure. The control and sham groups received normal saline before and after CCl4 exposure. To induce hepatotoxicity, animals in the sham and test groups were exposed against 100 l L-1 CCl4 for 45 min. The fish in all groups 1 h after CCl4 exposure were anesthetized and the blood samples were collected. Immediately the liver specimens were dissected out and were stored in 10 % formalin for further pathological studies. Determination of serum level of ALP and SGOT revealed that acute form of CCl4 exposure elevated significantly (P < 0.05 the serum level of either tested hepatic marker enzymes. While 3 days pretreatment with LPE prevented from ALP and SGOT enhancement. The pathological evaluation revealed that the CCl4 exposure resulted in a minor pathologic manifestation such as slight congestion, which the LPE pretreated groups showed the remarkable improvement. The anti-oxidant capacity of LPE was assayed by FRAP and DPPH methods. Both provided techniques showed that LPE exerts an excellent anti-oxidant effect. This data suggest that LPE exerts protective effect against CCl4-induced hepatotoxicity. Moreover, the hepatoprotective effect of LPE may attribute to its antioxidant capacity.

  18. Effects of carbon tetrachloride on oxidative stress, inflammatory response and hepatocyte apoptosis in common carp (Cyprinus carpio)

    Energy Technology Data Exchange (ETDEWEB)

    Jia, Rui [Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081 (China); Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 (China); Cao, Li-Ping; Du, Jin-Liang [Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 (China); International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 (China); Wang, Jia-Hao; Liu, Ying-Juan [Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081 (China); Jeney, Galina [National Agricultural Research Center, Research Institute for Fisherie and, Aquaculture, Anna Light 8, Szarvas 5440 (Hungary); Xu, Pao, E-mail: xup@ffrc.cn [Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081 (China); Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 (China); Yin, Guo-Jun, E-mail: yingj@ffrc.cn [Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081 (China); Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 (China); International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 (China)

    2014-07-01

    Highlights: • We explored the underlying toxicology of CCl{sub 4} at the cellular and molecular levels. • QRT-PCR detected the gene expression of NF-κB and inflammatory cytokines. • The apoptosis and necrosis occurred simultaneously in carp liver damage. • CCl{sub 4} activated the TNF-α/NF-κB and TRL4/NF-κB signaling pathways. - Abstract: In the present study, the cellular and molecular mechanism of carbon tetrachloride (CCl{sub 4})-induced hepatotoxicity in fish was investigated by studying the effects of CCl{sub 4} on the oxidative stress, inflammatory response and hepatocyte apoptosis. Common carp were given an intraperitoneal injection of 30% CCl{sub 4} in arachis oil (0.5 ml/kg body weight). At 72 h post-injection, blood were collected to measure glutamate pyruvate transaminase (GPT), glutamate oxalate transaminase (GOT), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione (GSH), total antioxidant capacity (T-AOC) and malondialdehyde (MDA), liver samples were taken to analyze toll-like receptor 4 (TLR4), cytochrome P450 2E1 (CYP2E1) and gene expressions of inflammatory cytokines and nuclear factor-κB (NF-κB/cREL). Cell viability and apoptosis were analyzed after treatment of the primary hepatocytes with CCl{sub 4} at 8 mM. The results showed that CCl{sub 4} significantly increased the levels of GPT, GOT, MDA, TLR4 and CYP2E1, reduced the levels of SOD, GPx, CAT, GSH and T-AOC, and up-regulated the gene expressions of NF-κB/cREL and inflammatory cytokines including tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), IL-1β, IL-6 and IL-12. In vitro, CCl{sub 4} caused a dramatic loss in cell viability and induced hepatocyte apoptosis. Overall results suggest that oxidative stress lipid peroxidation, and TNF-α/NF-κB and TRL4/NF-κB signaling pathways play important roles in CCl{sub 4}-induced hepatotoxicity in fish.

  19. Effects of carbon tetrachloride on oxidative stress, inflammatory response and hepatocyte apoptosis in common carp (Cyprinus carpio)

    International Nuclear Information System (INIS)

    Highlights: • We explored the underlying toxicology of CCl4 at the cellular and molecular levels. • QRT-PCR detected the gene expression of NF-κB and inflammatory cytokines. • The apoptosis and necrosis occurred simultaneously in carp liver damage. • CCl4 activated the TNF-α/NF-κB and TRL4/NF-κB signaling pathways. - Abstract: In the present study, the cellular and molecular mechanism of carbon tetrachloride (CCl4)-induced hepatotoxicity in fish was investigated by studying the effects of CCl4 on the oxidative stress, inflammatory response and hepatocyte apoptosis. Common carp were given an intraperitoneal injection of 30% CCl4 in arachis oil (0.5 ml/kg body weight). At 72 h post-injection, blood were collected to measure glutamate pyruvate transaminase (GPT), glutamate oxalate transaminase (GOT), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione (GSH), total antioxidant capacity (T-AOC) and malondialdehyde (MDA), liver samples were taken to analyze toll-like receptor 4 (TLR4), cytochrome P450 2E1 (CYP2E1) and gene expressions of inflammatory cytokines and nuclear factor-κB (NF-κB/cREL). Cell viability and apoptosis were analyzed after treatment of the primary hepatocytes with CCl4 at 8 mM. The results showed that CCl4 significantly increased the levels of GPT, GOT, MDA, TLR4 and CYP2E1, reduced the levels of SOD, GPx, CAT, GSH and T-AOC, and up-regulated the gene expressions of NF-κB/cREL and inflammatory cytokines including tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), IL-1β, IL-6 and IL-12. In vitro, CCl4 caused a dramatic loss in cell viability and induced hepatocyte apoptosis. Overall results suggest that oxidative stress lipid peroxidation, and TNF-α/NF-κB and TRL4/NF-κB signaling pathways play important roles in CCl4-induced hepatotoxicity in fish

  20. Occurrence of glucocorticoids discharged from a sewage treatment plant in Japan and the effects of clobetasol propionate exposure on the immune responses of common carp (Cyprinus carpio) to bacterial infection.

    Science.gov (United States)

    Nakayama, Kei; Sato, Kentaro; Shibano, Takazumi; Isobe, Tomohiko; Suzuki, Go; Kitamura, Shin-Ichi

    2016-04-01

    The present study evaluated the environmental risks to common carp (Cyprinus carpio) posed by glucocorticoids present in sewage treatment plant (STP) effluent. To gather information on the seasonal variations in glucocorticoid concentration, the authors sampled the effluent of a Japanese STP every other week for 12 mo. Six of 9 selected glucocorticoids were detected in the effluent, with clobetasol propionate and betamethasone 17-valerate detected at the highest concentrations and frequencies. The present study's results indicated that effluent glucocorticoid concentration may depend on water temperature, which is closely related to the removal efficiency of the STP or to seasonal variations in the public's use of glucocorticoids. In a separate experiment, to clarify whether glucocorticoids in environmental water increase susceptibility to bacterial infection in fish, the authors examined the responses to bacterial infection (Aeromonas veronii) of common carp exposed to clobetasol propionate. Clobetasol propionate exposure did not affect bacterial infection-associated mortality. In fish infected with A. veronii but not exposed to clobetasol propionate, head kidney weight and number of leukocytes in the head kidney were significantly increased (p < 0.05), whereas these effects were not observed in infected fish exposed to clobetasol. This suggests that clobetasol propionate alleviated bacterial infection-associated inflammation. Together, these results indicate that susceptibility to bacterial infection in common carp is not affected by exposure to glucocorticoids at environmentally relevant concentrations. Environ Toxicol Chem 2016;35:946-952. © 2015 SETAC. PMID:26126539

  1. The protective effect of silymarin on the carbon tetrachloride (CCl4)-induced liver injury in common carp (Cyprinus carpio).

    Science.gov (United States)

    Jia, Rui; Cao, Liping; Du, Jinliang; Xu, Pao; Jeney, Galina; Yin, Guojun

    2013-03-01

    Silymarin, a mixture of bioactive flavonolignans from the milk thistle (Silybum marianum), is traditionally used in herbal medicine to defend against various hepatotoxic agents. The aim of the present study was to evaluate the protective effect of silymarin against carbon tetrachloride (CCl4)-induced liver injury in fish. Common carp, with an average initial weight of 17.0 ± 1.1 g, were fed diet containing four doses of silymarin (0, 0.1, 0.5, and 1 g/kg diet) for 60 d. Fish were then given an intraperitoneal injection of CCl4 (30% in arachis oil) at a dose of 0.5 ml/kg body weight. At 72 h after CCl4 injection, blood and liver samples were collected for the analyses of serum biochemical parameters, liver index, peroxidation product, glutathione, and antioxidant enzyme activities. The results showed that administration of silymarin at 0.5 and 1 g/kg diet for 60 d prior to CCl4 intoxication significantly reduced the elevated activities of glutamate pyruvate transaminase, glutamate oxalate transaminase, lactate dehydrogenase (LDH), and increased the reduced levels of total protein and albumin in the serum. The reduced levels of liver index, superoxide dismutase, glutathione peroxidase, catalase, glutathione, and total antioxidant capacity were markedly increased, and malondialdehyde formation was significantly restrained in the liver. However, these parameters, except LDH, were not significantly changed in fish fed with silymarin at 0.1 g/kg diet. Based on the results, it can be concluded that silymarin has protective effect against CCl4-induced hepatotoxicity in fish. It is suggested that silymarin may be used as a hepatoprotective agent to prevent liver diseases in fish. PMID:23435858

  2. The influence of stocking density on the growth of common carp, Cyprinus carpio, in a recirculating aquaculture system

    Directory of Open Access Journals (Sweden)

    Ionica Enache

    2011-04-01

    Full Text Available The paper presents some aspects regarding the influence of stocking density on the breeding of the common carp in a recirculating aquaculture system (RAS. The experiment covered a 30 dayperiod. It was conducted in four aquaculture tanks, 500 L/tank, in the recirculating aquaculture pilot system of “Dunarea de Jos” University of Galați. Two kind of stocking density variants were compared: 64 kg/m3 in V1 (B1, B3 and 32 kg/m3 in V2 (B2, B4. The stocking density was: 491 fish in B1, average weight - 65 g/ fish; 245 fish in B2, average weight - 66 g/ fish; 211 fish in B3, average weight - 152 g/fish; 107 fish in B4, average weight - 150 g/fish. The technological indicators obtained revealed the following: the specific growth rate (SGR, calculated as a mean value on the two repetitions, was 1.28%/day in V1 and 1.49 %/day in V2, indicating better growth in V2; the food conversion ratio (FCR, calculated as the mean value of the two repetitions, was 1.28 in V1 and 1.06 in V2, expressing a higher efficiency in capitalization of food in V2. The mean biomass gain in V1 was 30.46 kg/m3 as compared to 18.55 kg/m3 in V2, almost perfectly correlated with the stocking density. The daily growth rate (DGR varied in a similar way, from 494.57 to 520.90 g/day in V1 and 302.4 to 315.9 g/day in V2. The experiment demonstrated a remarkable technological plasticity in both experimental variants, whereas, in terms of technological performance indicators, it was found that the application of lower stocking densities lead to higher crop biomass. The high stocking density means, therefore, large amounts of metabolic waste, removed from the breeding units through their effluent, the recirculating flow of which is 4m3/h, enough to ensure the whole volume of water in a growth tank to be changed every half hour.

  3. 锦鲤组织细胞体外培养的初步研究%Study on the in vitro Primary Culture of Tissue Cells from Ornamental Carp, Cyprinus carpio

    Institute of Scientific and Technical Information of China (English)

    付思思; 吴志新; 王敏; 汤蓉

    2012-01-01

    为建立来源于锦鲤的细胞系,本文采用组织块法,对来源于锦鲤(Cyprinus carpio)鳍条、吻端、肌肉、心脏、鳔、肠道、卵巢等组织的细胞进行原代培养和传代培养.在25℃下,添加20%胎牛血清、0.2μg/mL表皮生长因子(EGF)和25ng/mL成纤维生长因子(FGF)的L-15培养基中进行培养.结果显示,鳍条、心脏、鳔、吻端、肌肉、肠道、卵巢分别在原代培养第3、6、12、13、15、3、3天,有细胞从组织块迁出;对长至单层的鳍条、心脏、肌肉细胞进行传代培养,鳍条细胞已传至第39代,心脏和肌肉细胞分别传到第2代和第4代;鳍条、心脏和肌肉细胞分别呈现上皮细胞样、上皮细胞样和上皮细胞样与成纤维状混合型.第6代锦鲤鳍细胞的染色体计数,结果显示,细胞染色体数目分布范围为55~154条,2n=100.病毒敏感性实验,发现锦鲤鳍条细胞对草鱼出血病病毒(GCRV)和鲤春病毒血症病毒(SVCV)都敏感,且在24h内出现细胞病变(CPE);但对斑点叉尾鮰病毒(CCV)不敏感.锦鲤鳍条细胞系的建立为后期建立更多的锦鲤细胞系和鱼类病毒研究奠定了基础.%To establish cell lines from ornamental carp (Cyprinus carpio), primary cultures of fin, snout, muscle, heart, swim bladder, intestine and ovary of ornamental carp were initiated at 25℃ in L-15 medium supplemented with 20% fetal bovine serum, 0. 2μg/mL epidermal growth factor (EGF) and 25 ng/mL fibroblast growth factor (FGF) by using the tissue explants technique. The cells of fin, heart, swim bladder, snout, muscle intestine and ovary tissue migrated out around the tissues at day 3, 6, 12, 13, 15, 3 and 3 respectively. The confluent mpnolayers of cells were rinsed twice with 0. 25% trypsin-EDTA solution. The second generation of heart cells, the third generation of muscle cells and the thirty-ninth generation of fin cells were obtained. The morphologies of heart cells and fin cells were both epithe

  4. Effect of atrazine and chlorpyrifos on embryonic of common carp (Cyprinus carpio L%阿特拉津和毒死蜱对鲤胚胎发育的影响

    Institute of Scientific and Technical Information of China (English)

    韩英; 赵荣伟; 郝其睿; 姜旭阳; 张红

    2015-01-01

    研究旨在探讨不同浓度阿特拉津(Atrazine, ATR)、毒死蜱(Chlorpyrifos, CPF)及其混合物对鲤(Cyprinus carpio L.)胚胎发育、孵化率及抗氧化能力的影响。将鲤受精卵分别暴露在各自浓度为12.5、25、50、100、200μg·L-1的ATR、CPF及ATR-CPF 1��1混合液中,经96 h试验,观察发育形态,统计死亡率和畸形率,检测全鱼抗氧化能力。在暴露试验中,各处理组死亡率和畸形率随药物浓度增长呈上升趋势;初孵仔鱼体内SOD、GPx和CAT活性整体呈下降趋势。结果表明,阿特拉津和毒死蜱对鲤受精卵具有一定的致死、致畸效果,且两者生物毒性具有一定的叠加效应和协同作用,氧化胁迫是阿特拉津和毒死蜱对鲤发挥毒性作用的重要机制之一。%This study was aimed to explore the influence of different concentrations of atrazine (ATR), chlorpyrifos (CPF) and a mixture of them on embryonic development, hatchability and antioxidant capacity of common carp (Cyprinus carpio L.). The carp fertilized eggs was exposed to the concentrations of 12.5, 25, 50, 100, 200μg·L-1 ATR, CPF and ATR-CPF 1��1 mixture, respectively. After 96 h exposure, we observed their development of shape, and counted the death rate and aberration rate of the zygotes, detected antioxidant capacity of whole fish. In the exposure test, Mortality and malformation rate of treatment groups grow along with the drug concentration was on the rise;SOD, GPx and CAT activities in the newly hatched larvae of common carp as a whole were on the decline. The results indicated that ATR and CPF had some lethal and teratogenic effect on zygote of common carp, moreover, to a certain degree extent, the biotoxicity of these two toxic substances had additive effect and synergy, oxidative stress was one of the pivotal mechanism which ATR and CPF brought toxic effect to common carp.

  5. Genetic diversity of color phenotypes in the koi (Cyprinus carpio L.) as identified by molecular markers

    OpenAIRE

    Dani Bercovich; Sigal Korem; Lior Shauder; Gad Degani

    2012-01-01

    The common carp (Cyprinus carpio) has a large variety of strains. The more popular are the koi (Japanese ornamental carp), which are still bred today to generate creative colors and patterns, giving rise to multiple phenotypes. Since koi are in great demand, there is a challenge to determine the genetics defining their quality. Two methods: 1) direct sequencing of five candidate gene regions, i.e., mitochondrial (cytochrome b&l...

  6. 七种染料对鲤鱼肝微粒体芳烃羟化酶的诱导%THE INDUCTION OF ARYL HYDROCARBON HYDROXYLASE(AHH)OF CARP(CYPRINUS CARPIO) LIVER MICROSOME BY SEVEN DYES

    Institute of Scientific and Technical Information of China (English)

    徐楠; 王春霞; 莫争; 呼世斌

    2001-01-01

    The aryl hydrocarbon hydroxylase(AHH)activity of carp(Cyprinus Carpio)liver microsome induced by seven dyes was examined.The all tested dyes induced AHH activity to different extent.The AHH activity increased according to the increase of the dye concentration.The intense of AHH activity induced by seven dyes was as following order:acid red B>acid complex blue RRN>weak acid brilliant red B>reactive brilliant red K-2BP>reactive brilliant red K-2G,acid mordant red S-80>disperse red E-4B.The intense of AHH activity was related to the toxicity of the chemicals.%以鲤鱼肝微粒体为实验体系,研究了七种染料化合物对其芳烃羟化酶(AHH)的诱导,发现七种染料都可以诱导AHH的活性,随染料浓度增大AHH的活性升高.七种染料对AHH活性诱导能力大小为:酸性红B>派拉丁蓝RRN>普拉红B>活性艳红K-2BP>活性艳红K-2G,媒介大红S-80>分散红E-4B,与其毒性大小相关.

  7. Genetic diversity of Cyprinus carpio from Ohrid Lake, estimated by four microsatellite loci

    OpenAIRE

    ADIOLA BIBA; ANILA HODA; EDIT VARDHAMI

    2014-01-01

    Common carp (Cyprinus carpio L) is an important fish species of Ohrid lake. The aim of the present study was to evaluate the genetic diversity of common carp using microsatellite markers. A total of 30 individuals were genotyped for 4 microsatellite loci (MFW1, MFW6, MFW7, MFW18). All the microsatellite loci were polymorphic. A total of 84 alleles were distinguished. The allelic number varied from 19 to 23 with mean allelic number of 21. The effective number of alleles varied from 9.92 to 11....

  8. Effect of Dietary Alanyl-glutamine Supplementation on Growth Performance, Development of Intestinal Tract, Antioxidant Status and Plasma Non-speciifc Immunity of Young Mirror Carp (Cyprinus carpio L.)

    Institute of Scientific and Technical Information of China (English)

    Xu Hong; Zhu Qing; Wang Chang-an; Zhao Zhi-gang; Luo Ling; Wang Lian-sheng; Li Jin-nan; Xu Qi-you

    2014-01-01

    Exogenous alanyl-glutamine (Aln-Gln) was evaluated for its effects on growth performance, intestinal structure and function, antioxidant status and non-specific immunity of young carp (Cyprinus carpio L.). Six diets supplemented with 0, 2.5, 5.0, 7.5, 10.0, or 15.0 g• kg-1 of Aln-Gln were fed to fish for 12 weeks. Supplementation with 7.5, 10.0, or 15.0 g• kg-1 of Aln-Gln significantly increased weight gain rate (WGR), protein efficiency ratio (PER), but feed conservation rate (FCR) and survival were not affected (P>0.05). The intestinal fold height and number, digestive enzyme, Na+, K+-ATPase activities was found to be significantly high (P<0.05) with increasing dietary Aln-Gln supplementation up to 7.5 g• kg-1, but there were no significant differences for Aln-Gln supplementation from 7.5 to 15.0 g• kg-1. The glutathione peroxidase (GPX) activity, glutathione (GSH), superoxide dismutase (SOD) activity increased and malondialdehyde (MDA) levels decreased significantly (P<0.05) in the intestine, hepatopancreas, plasma and muscles. The plasma complement-3 (C3) and complement-4 (C4) levels were significantly (P<0.05) improved at 5.0 g• kg-1 level and decreased when over 7.5 g• kg-1. The plasma lysozyme (LSZ) activity increased significantly (P<0.05) at 7.5, 10.0, or 15.0 g• kg-1level. In summary, the results showed that Aln-Gln improved growth performance, development and function of the intestine, the activity of the antioxidant defense system and the plasma non-specific immunity of the carps. The optimal Aln-Gln level was 8.24 g• kg-1 diet for WGR based on broken-line regression model analysis.

  9. Evaluation of DNA Damage in Common Carp (Cyprinus carpio L. by Comet Assay for Determination of Possible Pollution in Lake Mogan (Ankara

    Directory of Open Access Journals (Sweden)

    İsmet Çok

    2011-01-01

    Full Text Available Contamination of the aquatic environment with various concentrations of pollutants results in unexpected threats to humans and wildlife. The consequences of exposure and metabolism of pollutants/xenobiotics, especially carcinogens and mutagens, can be suitably assessed by investigating severe events, such as DNA damage; for example, DNA adducts and DNA strand breaks. One of the commonly used techniques to detect DNA damage in aquatic organisms is single-cell gel electrophoresis (comet assay. This study was carried out using Cyprinus carpio in order to identify the possible pollution in Lake Mogan, near Ankara, Turkey, where the city's sewer system and pesticides used in agriculture are believed to be the common causes of pollution. From the comet assay, the tail length (μm, tail intensity (%, and tail moment values of fish caught from Lake Mogan were found to be 31.10 ± 10.39, 7.77 ± 4.51, 1.50 ± 1.48, respectively, whereas for clean reference sites they were found to be 22.80 ± 1.08, 3.47 ± 1.59, 0.40 ± 0.51, respectively. The values are statistically different from each other (p < 0.0001, p < 0.0001, and p < 0.0013, respectively. These results indicate that Lake Mogan may be polluted with substances that have genotoxic effects and constitute an early warning for the lake system. Further detailed research is needed to establish the source of the pollution and the chemicals responsible.

  10. Amelioration of cadmium-induced changes in biochemical parameters of the muscle of Common Carp (Cyprinus carpio by Vitamin C and Chitosan

    Directory of Open Access Journals (Sweden)

    Mahdi Banaee

    2015-12-01

    Full Text Available The aim of this study was to investigate the effects of administering antioxidants, including vitamin C and chitosan on oxidative stress markers in muscle as edible tissues of Cyprinus carpio exposed to cadmium chloride. In this experiment, by exposing to 0.2 mg/L cadmium chloride for 21 days, fish were fed a normal diet, diet containing chitosan (1000 mg/kg diet, vitamin C (1000 mg/kg diet or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA as well as biochemical parameters, including the activity of aspartate aminotransferase (AST, alanine aminotransferase (ALT, creatine phosphokinase (CPK, lactate dehydrogenase (LDH, and acetylcholinesterase (AChE were measured. Fish exposure to cadmium chloride significantly increased AST, LDH, CPK, catalase, and MDA activity, while it significantly decreased AST and AChE activity, and levels of total antioxidant in muscle cells. Administration of chitosan or vitamin C alone or in combination with each other to fish exposed to cadmium chloride was effective in regulating ALT, CPK, and catalase activity. Although administration of vitamin C and chitosan caused a significant decrease in MDA, AST and LDH, these enzymes were still significantly higher than those in the control group. Administration of vitamin C and chitosan had no significant effects on the activity of AChE and levels of total antioxidant. Although, chitosan alone could not prevent oxidative stress damages in muscle tissues of cadmium-treated fish, administration of vitamin C combined with chitosan may increase the efficiency of antioxidant defense system and improve the detoxification system in the muscles of fish exposed to cadmium chloride.

  11. Resistência da pele de carpa espelho (Cyprinus carpio specularis curtida pelas técnicas ao cromo e bioleather - DOI: 10.4025/actascianimsci.v26i4.1711 Resistance of the carp mirror skin (Cyprinus carpio specularis tanned by chromium and bioleather technique - DOI: 10.4025/actascianimsci.v26i4.1711

    Directory of Open Access Journals (Sweden)

    José Telmo Agostin Schmidt

    2004-04-01

    Full Text Available O objetivo do trabalho foi avaliar a técnica de curtimento bioleather e o curtimentocom sais de cromo, em peles de carpa espelho (Cyprinus carpio specularis. As peles foram retiradas com o auxílio de uma máquina, identificadas e congeladas. Em seguida, foram curtidas por meio de duas técnicas diferentes. O passo seguinte foi retirada dos corpos de prova, que foram levados para o laboratório, climatizado em torno de 23ºC e umidade relativa, do ar de 50%, conforme NBR 10455 (1988, por 24 horas. Para os testes de resistência à tração, ao alongamento e à determinação da força de rasgamento progressivo, utilizou-se o dinamômetro EMIC. Foram retiradas amostras da região dorsal do couro para análise de microscopia eletrônica de varredura (MEV. As amostras foram secas em ponto crítico com CO2 e metalizadas com íons de ouro-paládio. Em seguida as amostras foram elétron-micrografados com o JEOL-JSM 5410. Observou-se o entrelaçamento e sobreposição das camadas das fibras colágenas, que proporcionam maior resistência ao couro, constatada pelos testes realizados. Os couros curtidos com a técnica bioleather apresentaram maiores valores no teste de tração (20,45 N/mm2 em comparação ao cromo (12,32 N/mm2. Não houve diferença na elongação entre os tratamentos (bioleather = 72,52%; cromo = 84,74%. Para o teste de rasgamento progressivo, o curtimento ao cromo foi superior (26,28 N/mm, comparado ao bioleather (11,96 N/mm. Entretanto, os dois curtimentos podem ser utilizados nas peles dessa espécie de peixe para a confecção de vestuários.The objective of the work was to evaluate the tanning technique to bioleather and with chromium salts, in carp mirror skins (Cyprinus carpio specularis. The skins wore removed by means of a machine, identified and congealed. Next they were tanned by the two techniques. The test bodies wore removed and taken to the laboratory climatized around 23ºC and relative humidity of the 50% air, as ABNT

  12. 盐碱池塘养殖鲤肠道菌群的分子分析%Molecular Analysis of Intestinal Microflora in Common Carp(Cyprinus carpio L.) from Saline-alkali Ponds

    Institute of Scientific and Technical Information of China (English)

    黄进强; 刘哲; 王建福; 蔡原; 冯志云

    2013-01-01

    为了了解盐碱池塘养殖鲤肠道细菌群落组成及多样性,提取鲤(Cyprinus carpio L.)肠道细菌基因组DNA,选用细菌通用引物对16S rRNA基因进行了PCR扩增,构建了细菌16S rRNA基因克隆文库.通过对阳性克隆子进行限制性片段长度多态性分析(RFLP),选出有代表性的克隆子进行测序、BLAST比对分析和构建系统发育树.本研究从16S rRNA基因文库中共筛选出176个阳性克隆,经RFLP分析得到28个不同分类操作单元(operational taxonomic unite,OTU)(GenBank登录号:JX262557~JX262584),文库覆盖度为88.6%.16S rRNA序列系统发育分析发现,盐碱塘养殖鲤肠道细菌归属于变形细菌门(Proteobacteria)(包含Alpha和Gamma亚群)、厚壁细菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和梭杆菌门(Fusobacteria)4个门,分别占克隆总数的89.9%、7.9%、1.1%和1.1%.其中,变形细菌门的α-变形菌纲(占克隆总数的88.1%)为优势类群,气单胞菌属为优势菌属.本研究揭示了盐碱池塘养殖条件下健康鲤肠道细菌群落组成.%In order to investigate composition and diversity of intestinal bacteria of common carp(Cyprinus carpio L.) reared in saline-alkaline ponds,the 16S rRNA genes were amplified from the total DNA of intestinal bacteria in common carp by PCR with bacteria-specific primers and a clone library was constructed.Positive clones were analyzed by restriction fragment length polymorphism(RFLP),and the representative clones with unique patterns were sequenced,BLAST and then constructed phylogenetic tree.A total of 28 Operational Taxonomic Units (OTUs)(GenBank accession No.JX262557~JX262584) were sequenced from 176 clones of the clone library.The clones coverage value (C=88.6%) and rarefaction analysis both showed that the clone library covered the majority intestinal bacteria.Sequence alignment showed that the most bacteria exhibited high similarity (>98%) with known bacterial 16S rRNA genes retrieved from

  13. Comparison of rearing performances and intermuscular bone number in the mirror and nude genotypes of common carp (Cyprinus carpio L.) in a controlled field test in Madagascar

    OpenAIRE

    Andria-Mananjara, Diana Edithe; Rasamoelina, Harena; Vandeputte, Marc

    2016-01-01

    Nude carps, a genotype of common carp which are devoid of scales, have been banned from farmed populations of carp in Europe due to both the lethal effect of the N (nude) gene when in homozygous state, and to the negative pleiotropic effects on growth and survival (especially in harsh conditions) of this same gene in the heterozygous state, which produces the nude phenotype. In Madagascar, where climatic variations are less extreme than in Eastern Europe, the nude phenotype is valued both by ...

  14. Substrate mobilization and hormonal changes in rainbow trout (Oncorhynchus mykiss, L) and common carp (Cyprinus carpio, L) during deep hypoxia and subsequent recovery

    NARCIS (Netherlands)

    VanRaaij, MTM; VandenThillart, GEEJM; Vianen, GJ; Pit, DSS; Balm, PHM; Steffens, B

    1996-01-01

    Common carp (at 20 degrees C) and rainbow trout (at 15 degrees C) were fitted with an indwelling cannula in the dorsal aorta. The fish were exposed to a controlled decline of water pO(2) followed by 90 min deep hypoxia at 0.3 kPa (carp) or 4.8 kPa (trout). Thereafter, normoxic recovery was monitored

  15. Effects of common carp Cyprinus carpio (L.) and feed addition in rohu Labeo rohita (Hamilton) ponds on nutrient partitioning among fish, plankton and benthos

    NARCIS (Netherlands)

    Rahman, M.M.; Verdegem, M.C.J.; Nagelkerke, L.A.J.; Wahab, M.A.; Milstein, A.; Verreth, J.A.J.

    2008-01-01

    The effects of introducing common carp (CC) and of adding artificial feed to fertilized rohu ponds on water quality and nutrient accumulation efficiency were studied. All ponds were stocked with 15 000 rohu ha¿1. Treatments included ponds with rohu alone, rohu plus 5000 common carp ha¿1 and rohu plu

  16. Hepatoprotective and antioxidant effects of Azolla microphylla based gold nanoparticles against acetaminophen induced toxicity in a fresh water common carp fish (Cyprinus carpio L.

    Directory of Open Access Journals (Sweden)

    Selvaraj Kunjiappan

    2015-04-01

    Conclusion: Azolla microphylla phytochemically synthesized GNaP protects liver against oxidative damage and tissue damaging enzyme activities and could be used as an effective protector against acetaminophen-induced hepatic damage in fresh water common carp fish.

  17. The slaughtering value of all-female and bisexual populations of common carp (Cyprinus carpio L.) cultured in pond conditions of the central Europe

    Czech Academy of Sciences Publication Activity Database

    Kocour, Martin; Linhart, Otomar; Gela, David; Rodina, Marek; Flajšhans, Martin

    Chile : Puerto Varas, 2003. s. 120. [Genetics in Aquaculture /8./. 09.11.2003-15.11.2003, Puerto Varas] Institutional research plan: CEZ:AV0Z5045916 Keywords : common carp Subject RIV: ED - Physiology

  18. The growth characteristics of common carp (Cyprinus carpio in the northern part of the Small Island of Brăila Natural Park

    Directory of Open Access Journals (Sweden)

    Daniela C. Gheorghe

    2011-04-01

    Full Text Available . In this paper are presented the results of the growth parameters for the carp population. The study was realized in the 2006-2009 period on a total number of 367 carp specimens caught into the Danube river km 170-196 (the arms: Cravia, Calia, and Fundu Mare Island. In order to estimate the population growth characteristic of the carp population the following relationship was determined: length - weight (L-W using equation W = a * Lb . The value of the coefficient b indicates an allometric increase, meaning the length increases faster than weight. Moreover, the good value of this coefficient, considered also as a condition factor (b = 2.845, shows us good growth conditions for this species in the researched area. Estimation of the growth parameters (von Bertalanffy L∞, k, to, led to obtaining of comparable values with other nearby regions carp populations.

  19. Enzyme-linked immunosorbent assay of changes in serum levels of growth hormone (cGH) in common carps (Cyprinus carpio)

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The aim of the present study was to purify the common native carp growth hormone (ncGH), produce monoclonal antibodies (mAbs) to common native carp growth hormone (ncGH), and further enhance the sensitivity of enzyme-linked immunosorbent assays (ELISA) for ncGH. Additionally, we investigated changes in serum ncGH levels in carps raised in different environmental conditions. The recombinant grass carp (Ctenopharyngodon idella) growth hormone was purified and used as antigen to immunize the rabbit. The natural ncGH was isolated from the pituitaries of common carp. SDS-PAGE and Western blot utilizing the polyclonal anti-rgcGH antibody confirmed the purification of ncGH from pituitaries. Purified ncGH was then used as an immunogen in the B lymphocyte hybridoma technique. A total of 14 hybridoma cell lines (FMU-cGH 1-14) were established that were able to stably secrete mAbs against ncGH. Among them, eight clones (FMU-cGH1-6, 12 and 13) were successfully used for Western blot while nine clones (FMU-cGH 1-7, 9 and 10) were used in fluorescent staining and immunohistochemistry. Epitope mapping by competitive ELISA demonstrated that these mAbs recognized five different epitopes. A sensitive sandwich ELISA for detection of ncGH was developed using FMU-cGH12 as the coating mAb and FMU-cGH6 as the enzyme labeled mAb. This detection system was found to be highly stable and sensitive, with detection levels of 70 pg/mL. Additionally, we found that serum ncGH levels in restricted food group and in the net cage group increased 6.9-and 5.8-fold, respectively, when compared to controls, demonstrating differences in the GH stress response in common carp under different living conditions.

  20. Enzyme-linked immunosorbent assay of changes in serum levels of growth hormone (cGH) in common carps (Cyprinus carpio)

    Institute of Scientific and Technical Information of China (English)

    WU Gang; CHEN LiHua; ZHONG Shan; LI Qi; SONG ChaoJun; JIN BoQuan; ZHU ZuoYan

    2008-01-01

    The aim of the present study was to purify the common native carp growth hormon.e (ncGH), produce monoclonal antibodies (mAbs) to common native carp growth hormone (ncGH), and further enhance the sensitivity of enzyme-linked immunosorbent assays (ELISA) for ncGH. Additionally, we investigated changes in serum ncGH levels in carps raised in different environmental conditions. The recombinant grass carp (Ctenopharyngodon idella) growth hormone was purified and used as antigen to immunize the rabbit. The natural ncGH was isolated from the pituitaries of common carp. SDS-PAGE and Western blot utilizing the polyclonal anti-rgcGH antibody confirmed the purification of ncGH from pituitaries.Purified ncGH was then used as an immunogen in the B lymphocyte hybridoma technique. A total of 14 hybridoma cell lines (FMU-cGH 1-14) were established that were able to stably secrete mAbs against ncGH. Among them, eight clones (FMU-cGH1-6, 12 and 13) were successfully used for Western blot while nine clones (FMU-cGH 1 -7, 9 and 10) were used in fluorescent staining and immunohistochemIstry. Epitope mapping by competitive ELISA demonstrated that these mAbs recognized five different epitopes. A sensitive sandwich ELISA for detection of ncGH was developed using FMU-cGH12 as the coating mAb and FMU-cGH6 as the enzyme labeled mAb. This detection system was found to be highly stable and sensitive, with detection levels of 70 pg/mL. Additionally, we found that serum ncGH levels in restricted food group and in the net cage group increased 6.9- and 5.8-fold, respectively, when compared to controls, demonstrating differences in the GH stress response in common carp under different living conditions.

  1. 饲料脂肪水平对锦鲤体色和几项免疫指标的影响%Effects of dietary lipid levels on body pigmentation and immune indicators in Koi carp Cyprinus carpio

    Institute of Scientific and Technical Information of China (English)

    孙向军; 罗琳; 姜志强; 孙砚胜; 史东杰; 梁拥军

    2011-01-01

    研究了在螺旋藻添加的质量分数为12%时,饲料脂肪水平(5.36%、7.74%、10.52%、12.85%和15.45%均为质量分数)对红白锦鲤Ornmmental carp幼鱼(初始体质量为5.85g±0.19 g)体色和免疫指标的影响.每组设3个重复,每个重复组饲养10尾锦鲤,表观饱食投喂60d.结果表明:当饲料中脂肪水平为10.52%时,试验鱼皮肤中类胡萝卜素含量显著高于其它试验组(P<0.05),体表红质的α*值最高;试验鱼肝胰脏中超氧化物歧化酶、过氧化氢酶的活力随脂肪水平的升高而下降,饲料脂肪水平对碱性磷酸酶、乳酸脱氢酶等免疫指标没有产生显著影响(P>0.05).上述研究说明,在配合饲料中添加一定量的螺旋藻时,锦鲤着色效果最好时的适宜饲料脂肪水平为10.52%,提高饲料脂肪水平不会持续提高锦鲤对螺旋藻的利用效率.%A feeding trial with triplication was conducted to investigate the effects of dietary lipid levels on body pigmentation and immune indicators in koi carp Cyprinus carpio. The koi carp with initial body weight of (5. 85±0. 19) g were fed five diets containing 12% Spirulina and different lipid levels (5.36% ,7.74% ,10.52% ,12.85%, and 15.45% ) to apparent satiation at 15-25℃ for 60 days. The results showed that there was significantly higher concentration of carotenoid in the skin of the fish fed the diet containing 10. 52% lipid (with the maximal value) than that in the fish fed the other experimental diets (P<0. 05). The superoxide dismutase ( SOD) and catalase ( CAT) activities were found to be decreased with the increase in dietary lipid levels. No significant differences in alkaline phosphatase ( AKP) ,and lactate dehydrogenase (LDH) activities were observed in the fish fed different diets (P>0.05). It was concluded that the diet containing 10.52% lipid level showed improvement of body color in the koi carp fed the diet containing equivalent Spirulina.

  2. Effects of bisphenol A-related diphenylalkanes on vitellogenin production in male carp (Cyprinus carpio) hepatocytes and aromatase (CYP19) activity in human H295r adrenocortical carcinoma cells

    International Nuclear Information System (INIS)

    The present study investigated the effects of the known xenoestrogen bisphenol A (BPA) relative to eight BPA-related diphenylalkanes on estrogen receptor (ER)-mediated vitellogenin (vtg) production in hepatocytes from male carp (Cyprinus carpio), and on aromatase (CYP19) activity in the human adrenocortical H295R carcinoma cell line. Of the eight diphenylalkanes, only 4,4'-(hexafluoropropylidene)diphenol (BHF) and 2,2'-bis(4-hydroxy-3-methylphenyl)propane (BPRO) induced vtg, i.e., to a maximum of 3% to 4% (at 100 μM) compared with 8% for BPA relative to the maximum induction by 17β-estradiol (E2, 1 μM). Bisphenol A diglycidyl ether (BADGE) was a potent antagonist of vtg production with an IC50 of 5.5 μM, virtually 100% inhibition of vtg at 20 μM, and an inhibitive (IC50) potency about one-tenth that of the known ER antagonist tamoxifen (IC50, 0.6 μM). 2,2'-Diallyl bisphenol A, 4,4'-(1,4-phenylene-diisopropylidene)bisphenol, BPRO, and BHF were much less inhibitory with IC50 concentrations of 20-70 μM, and relative potencies of 0.03 and 0.009 with tamoxifen. Bisphenol ethoxylate showed no anti-estrogenicity (up to 100 μM), and 4,4'-isopropylidene-diphenol diacetate was only antagonistic at 100 μM. When comparing the (anti)estrogenic potencies of these bisphenol A analogues/diphenylalkanes, anti-estrogenicity occurred at lower concentrations than estrogenicity. 4,4'-Isopropylidenebis(2,6-dimethylphenol) (IC50, 2.0 μM) reduced E2-induced (EC50, 100 nM) vtg production due to concentration-dependent cytotoxicity as indicated by a parallel decrease in MTT activity and vtg, whereas the remaining diphenylalkanes did not cause any cytotoxicity relative to controls. None of the diphenylalkanes (up to 100 μM) induced EROD activity indicating that concentration-dependent, CYP1A enzyme-mediated metabolism of E2, or any Ah-receptor-mediated interaction with the ER, was not a likely explanation for the observed anti-estrogenic effects. At concentrations as great as 100

  3. Reproductive Responses of Common Carp Cyprinus carpio in Cages to Influent of the Las Vegas Wash in Lake Mead, Nevada, from late Winter to early Spring

    Science.gov (United States)

    To investigate the potential for contaminants in Las Vegas Wash (LW) influent to produce effects indicative of endocrine disruption in vivo, adult male and female common carp were exposed in cages for 42-48 d at four sites and two reference locations in Lake Mead.

  4. Results of comparative growing test of all-female and bisexual population in two-year-old common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Kocour, Martin; Linhart, Otomar; Gela, David

    2003-01-01

    Roč. 11, - (2003), s. 369-378. ISSN 0967-6120 R&D Projects: GA ČR GA523/97/S056 Institutional research plan: CEZ:AV0Z5045916 Keywords : all-female * common carp * genetics Subject RIV: ED - Physiology Impact factor: 0.446, year : 2003

  5. Effects of the lipid regulating drug clofibric acid on PPARα-regulated gene transcript levels in common carp (Cyprinus carpio) at pharmacological and environmental exposure levels

    Energy Technology Data Exchange (ETDEWEB)

    Corcoran, Jenna, E-mail: J.F.Corcoran@exeter.ac.uk [University of Exeter, Biosciences, College of Life & Environmental Sciences, Exeter EX4 4QD (United Kingdom); Winter, Matthew J., E-mail: M.Winter@exeter.ac.uk [AstraZeneca Global Environment, Brixham Laboratory, Freshwater Quarry, Brixham TQ5 8BA (United Kingdom); Lange, Anke, E-mail: A.Lange@exeter.ac.uk [University of Exeter, Biosciences, College of Life & Environmental Sciences, Exeter EX4 4QD (United Kingdom); Cumming, Rob, E-mail: Rob.Cumming@astrazeneca.com [AstraZeneca Global Environment, Brixham Laboratory, Freshwater Quarry, Brixham TQ5 8BA (United Kingdom); Owen, Stewart F., E-mail: Stewart.Owen@astrazeneca.com [AstraZeneca Global Environment, Brixham Laboratory, Freshwater Quarry, Brixham TQ5 8BA (United Kingdom); Tyler, Charles R., E-mail: C.R.Tyler@exeter.ac.uk [University of Exeter, Biosciences, College of Life & Environmental Sciences, Exeter EX4 4QD (United Kingdom)

    2015-04-15

    Highlights: • CFA appears to have a low propensity to bioconcentrate and has a plasma half-life of <4 days in carp. • CFA increases levels of mRNA of a number of genes known to be regulated by PPARα in mammals. • PPARα activation changes levels of mRNA of genes involved with several detoxification/ biotransformation system components in carp. • CFA alters levels of mRNA and activity of the inducible β-oxidation pathway enzyme Acox1, a known indicator of peroxisome proliferator exposure. - Abstract: In mammals, the peroxisome proliferator-activated receptor α (PPARα) plays a key role in regulating various genes involved in lipid metabolism, bile acid synthesis and cholesterol homeostasis, and is activated by a diverse group of compounds collectively termed peroxisome proliferators (PPs). Specific PPs have been detected in the aquatic environment; however little is known on their pharmacological activity in fish. We investigated the bioavailability and persistence of the human PPARα ligand clofibric acid (CFA) in carp, together with various relevant endpoints, at a concentration similar to therapeutic levels in humans (20 mg/L) and for an environmentally relevant concentration (4 μg/L). Exposure to pharmacologically-relevant concentrations of CFA resulted in increased transcript levels of a number of known PPARα target genes together with increased acyl-coA oxidase (Acox1) activity, supporting stimulation of lipid metabolism pathways in carp which are known to be similarly activated in mammals. Although Cu,Zn-superoxide dismutase (Sod1) activity was not affected, mRNA levels of several biotransformation genes were also increased, paralleling previous reports in mammals and indicating a potential role in hepatic detoxification for PPARα in carp. Importantly, transcription of some of these genes (and Acox1 activity) were affected at exposure concentrations comparable with those reported in effluent discharges. Collectively, these data suggest that CFA

  6. Effects of the lipid regulating drug clofibric acid on PPARα-regulated gene transcript levels in common carp (Cyprinus carpio) at pharmacological and environmental exposure levels

    International Nuclear Information System (INIS)

    Highlights: • CFA appears to have a low propensity to bioconcentrate and has a plasma half-life of <4 days in carp. • CFA increases levels of mRNA of a number of genes known to be regulated by PPARα in mammals. • PPARα activation changes levels of mRNA of genes involved with several detoxification/ biotransformation system components in carp. • CFA alters levels of mRNA and activity of the inducible β-oxidation pathway enzyme Acox1, a known indicator of peroxisome proliferator exposure. - Abstract: In mammals, the peroxisome proliferator-activated receptor α (PPARα) plays a key role in regulating various genes involved in lipid metabolism, bile acid synthesis and cholesterol homeostasis, and is activated by a diverse group of compounds collectively termed peroxisome proliferators (PPs). Specific PPs have been detected in the aquatic environment; however little is known on their pharmacological activity in fish. We investigated the bioavailability and persistence of the human PPARα ligand clofibric acid (CFA) in carp, together with various relevant endpoints, at a concentration similar to therapeutic levels in humans (20 mg/L) and for an environmentally relevant concentration (4 μg/L). Exposure to pharmacologically-relevant concentrations of CFA resulted in increased transcript levels of a number of known PPARα target genes together with increased acyl-coA oxidase (Acox1) activity, supporting stimulation of lipid metabolism pathways in carp which are known to be similarly activated in mammals. Although Cu,Zn-superoxide dismutase (Sod1) activity was not affected, mRNA levels of several biotransformation genes were also increased, paralleling previous reports in mammals and indicating a potential role in hepatic detoxification for PPARα in carp. Importantly, transcription of some of these genes (and Acox1 activity) were affected at exposure concentrations comparable with those reported in effluent discharges. Collectively, these data suggest that CFA

  7. Description of an as yet unclassified DNA virus from diseased Cyprinus carpio species.

    Science.gov (United States)

    Hutoran, Marina; Ronen, Ariel; Perelberg, Ayana; Ilouze, Maya; Dishon, Arnon; Bejerano, Izhak; Chen, Nissim; Kotler, Moshe

    2005-02-01

    Numerous deaths of koi and common carp (Cyprinus carpio) were observed on many farms throughout Israel, resulting in severe financial losses. The lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. Diseased fish exhibit fatigue and gasping movements in shallow water. Infected fish had interstitial nephritis and gill necrosis as well as petechial hemorrhages in the liver and other symptoms that were not consistent with viral disease, suggesting a secondary infection. Here we report the isolation of carp nephritis and gill necrosis virus (CNGV), which is the etiologic agent of this disease. The virus propagates and induces severe cytopathic effects by 5 days postinfection in fresh koi or carp fin cell cultures (KFC and CFC, respectively), but not in epithelioma papillosum cyprini cells. The virus harvested from KFC cultures induced the same clinical signs, with a mortality of 75 to 95%, upon inoculation into naive koi and common carp. Using PCR, we provide final proof that the isolated virus is indeed the etiologic agent of food and ornamental carp mortalities in fish husbandry. Electron microscopy revealed viral cores with icosahedral morphology of 100 to 110 nm that resembled herpesviruses. Electron micrographs of purified pelleted CNGV sections, together with viral sensitivities to ether and Triton X-100, suggested that it is an enveloped virus. However, the genome of the isolated virus is a double-stranded DNA (dsDNA) molecule of 270 to 290 kbp, which is larger than known herpesviruses. The viral DNA seems highly divergent and bears only small fragments (16 to 45 bp) that are similar to the genomes of several DNA viruses. Nevertheless, amino acid sequences encoded by CNGV DNA fragments bear similarities primarily to members of the Poxviridae and Herpesviridae and to other large dsDNA viruses. We suggest, therefore, that the etiologic agent of this disease may

  8. Association study between single nucleotide polymorphisms in leptin and growth traits in Cyprinus carpio var. Jian.

    Science.gov (United States)

    Tang, Y; Li, H; Li, J; Yu, F; Yu, J

    2016-01-01

    Leptin is a hormone that affects the regulation of body weight, energy expenditure, fat metabolism, food intake, and appetite. In this study, we cloned the jlLEP-A1 and jlLEP-A2 genes in Jian carp (Cyprinus carpio var. Jian) and performed an association analysis between identified polymorphisms and growth traits. Three polymorphisms in exons of jlLEP-A1 (A1-T113C) and jlLEP-A2 (A2-G415A and A2-G427A) were identified, and genotyped by the polymerase chain reaction - restriction fragment length polymorphism method in 263 female and 294 male Jian carp. All three SNPs were missense mutations. Association analysis revealed that the three SNPs were significantly associated with growth traits in male Jian carp. Only SNP A1-T113C was significantly associated with growth traits in female Jian carp. Analysis of diplotypes derived from jlLEP-A2 SNPs revealed an association with growth traits in male but not female Jian carp. These results demonstrate that polymorphisms in the leptin gene are associated with growth traits and may be used for marker-assisted selection programs in Jian carp breeding and production. PMID:27525905

  9. Early intrauterine embryonic development in Khawia sinensis Hsu, 1935 (Cestoda, Caryophyllidea, Lytocestidae), an invasive tapeworm of carp (Cyprinus carpio): an ultrastructural study

    Czech Academy of Sciences Publication Activity Database

    Bruňanská, M.; Mackiewicz, J. S.; Młocicki, D.; Świderski, Z.; Nebesářová, Jana

    2012-01-01

    Roč. 110, č. 2 (2012), s. 1009-1017. ISSN 0932-0113 Institutional support: RVO:60077344 Keywords : ATRACTOLYTOCESTUS-HURONENSIS ANTHONY * WENYONIA-VIRILIS WOODLAND * DIDYMOBOTHRIUM-RUDOLPHII MONTICELLI * FARMED COMMON CARP * ONCOSPHERAL ENVELOPES * VITELLOGENESIS * PARASITE * CESTOIDEA * EUCESTODA Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.852, year: 2012 http://link.springer.com/content/pdf/10.1007%2Fs00436-011-2590-2

  10. Environmental Degradation in a Eutrophic Shallow Lake is not Simply Due to Abundance of Non-native Cyprinus carpio

    Science.gov (United States)

    Ramírez-Herrejón, Juan P.; Mercado-Silva, Norman; Balart, Eduardo F.; Moncayo-Estrada, Rodrigo; Mar-Silva, Valentín; Caraveo-Patiño, Javier

    2015-09-01

    Non-native species are often major drivers of the deterioration of natural ecosystems. The common carp Cyprinus carpio are known to cause major changes in lentic systems, but may not be solely responsible for large scale changes in these ecosystems. We used data from extensive collection efforts to gain insight into the importance of carp as drivers of ecosystem change in Lake Patzcuaro, Mexico. We compared the structure (fish density, biomass, diversity, and evenness) of fish assemblages from six Lake Patzcuaro sites with different habitat characteristics. Intersite comparisons were carried out for both wet and dry seasons. We explored the relationships between non-carp species and carp; and studied multivariate interactions between fish abundance and habitat characteristics. From a biomass perspective, carp was dominant in only four of six sites. In terms of density, carp was not a dominant species in all sites. Further, carp density and biomass were not negatively related to native species density and biomass, even when carp density and biomass were positively correlated to water turbidity levels. Carp dominated fish assemblages in the shallowest sites with the highest water turbidity, plant detritus at the bottom, and floating macrophytes covering the lake surface. These results suggest that the effect of carp on fish assemblages may be highly dependent on habitat characteristics in Lake Patzcuaro. Watershed degradation, pollution, water level loss, and other sources of anthropogenic influence may be more important drivers of Lake Patzcuaro degradation than the abundance of carp.

  11. 虾青素对锦鲤血液及抗氧化指标的影响%Effect of dietary axtaxanthin on hematic and immune parameters of Ornamental Carp (Cyprinus carpio L.)

    Institute of Scientific and Technical Information of China (English)

    崔培; 刘芳; 杨广; 姜志强

    2013-01-01

    The study was to reasearch effects of dietary axtaxanthin on body pigmentation and growth of ommmental carp (Cyprinus carpio L.) (initial mean body weight:7.31±0.12).Fish was fed with different axtaxanthin dosage (0,100,400、700、1 000、1 300、1 600 mg· kg-1) and fed to apparent satiety to these groups of 10 fishes for 60 days.The results showed that the numbers of red cells and the hemoglobin content were increased significantly for feeding 700-1 600 mg· kg-1 axtaxanthin while no effects on ALT.The GLU increased obviously in the 700 mg·kg-1 group and the activity of AKP was significantly higher than that in control group.The activity of SOD in X2 group was lower obviously than that in control and other groups except X1 group.No significant effects on LDH and CAT.The results showed that dietary astaxanthin was effective in improving immunity of ommmental carp and the recommended dietary dosage was 700 mg·kg-1.%以初始体重为(7.31±0.12)g的红白锦鲤幼鱼为试验对象,研究虾青素不同添加量(0、100、400、700、1 000、1 300、1 600 mg· kg-1)对锦鲤血液和免疫指标的影响.每组设3个平行,每个平行饲养10尾鱼,表现饱食投喂60d.结果表明,在饲料中添加700~1 600 mg·kg-1 (X3~X6组)虾青素,可显著提高锦鲤血红蛋白含量和红细胞数,在此范围对谷丙转氨酶(ALT)活力无显著影响,添加700 mg·kg-1 (X3组)虾青素,可以显著提高锦鲤血糖(GLU)含量,同时,碱性磷酸酶(AKP)活性显著高于对照组.超氧化物歧化酶(SOD)活力在虾青素添加量为400 mg· kg-1(X2组)时,显著低于对照组和除100 mg·kg-1(X1组)外的其他试验组(P<0.05).添加虾青素没有对锦鲤乳酸脱氢酶(LDH)和过氧化氢酶(CAT)产生影响.上述研究说明,在饲料中添加虾青素可提高锦鲤免疫功能,综合经济效益角度建议,饲料中虾青素的适宜添加量为700 mg·kg-1.

  12. DI-2-ETHYLHEXYL PHTHALATE AND DI-N-BUTYL PHTHALATE IN TISSUES OF COMMON CARP (Cyprinus Carpio L.) AFTER HARVEST AND AFTER STORAGE IN FISH STORAGE TANKS

    OpenAIRE

    Vlasta Stancová; Lenka Puškárová; Alžbeta Jarošová

    2011-01-01

    The aim of the present study was to determine whether the influence of fish pond and fish storage tank conditions change the content of phthalic acid esters (di-n-butyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP)) in the carcass of the Common carp. Samples obtained from the autumn harvest of two fish ponds (R1 and R2) in 2007 and 2010 from the South Moravia and after a seven-week-long storage in fish storage tanks were analyzed. It was found that in the samples (2007) from both fish ...

  13. Assessment of the effect of temperature on the carp physiology (Cyprinus carpio, L., 1758 fed with probiotics in condition of a recirculating aquaculture system

    Directory of Open Access Journals (Sweden)

    Elena BOCIOC

    2015-10-01

    Full Text Available In order to establish the effect of temperature on the physiology of the carp, special attention was awarded to leukograma and absolute number of leukocytes of carp blood. Body's reaction to stress factors action involves physiological changes, including changes in blood composition and immune mechanisms. The hematological analysis has been effectuated in the experiment that took place during 60 days, in four breeding units of 500 litters in volume each. Four kinds of variants were compared: V1-pellets with 30% crude protein, without probiotics; V2-pellets with 30% crude protein, with probiotics of 2.24×109 CFU/kg food; V3-pellets with 30% crude protein, with probiotics of 3.84×109 CFU/kg food; and V4-pellets with 30% crude protein, with probiotics of 7.04×109 CFU/kg food. We used BioPlus®2B probiotics (a mixture of Bacillus licheniformis (DSM 5749 and Bacillus subtilis (DSM 5750. The microscopic examination of blood smears, which were coloured with May-Grunewald Giemsa panoptic method (both at the beginning and at the end of each experimental stage, we found that lymphocytes were predominant in comparison with other types of leukocytes, the absolute number being: V1 73.3 – 85.9%; V2 65.3 – 84.7%; V3 67.0 – 87.5%; V4 71.3 – 88.3%. It was noticed that eosinophilic granulocytes and basophils from the fish blood, were presented in a low number (0.1–0.8%, (0.2 – 0.4% respectively, also at the beginning of the experiment and in the end of the two phases. The glucose concentration in blood registered higher values, across to normal values reported by literature for carp, also in control group (V1 – 101.8 mg/dl as well as variants with different concentrations of probiotic (V2 – 104.6 mg/dl, V3 – 102.2 mg/dl, V4 – 116.2 mg/dl. The mean values of protein level were in the normal limits for carp, between 3.9 g/dl and 4.9 g/dl, aspect which suggests that fish present a normal physiological condition. In conclusion, probiotics can

  14. Cloning and expression analysis in mature individuals of two chicken type-Ⅱ GnRH (cGnRH-Ⅱ) genes in common carp (Cyprinus carpio )

    Institute of Scientific and Technical Information of China (English)

    LI Shuangfei; HU Wei; WANG Yaping; ZHU Zuoyan

    2004-01-01

    Gonadotropin-releasing hormone(GnRH)is a conservative neurodecapeptide family,which plays a crucial role in regulating the gonad development and in controlling the final sexual maturation in vertebrate.Two differing cGnRH-Ⅱ cDNAs of common carp,namely cGnRH-Ⅱ cDNA1 and cDNA2,were firstly cloned from the brain by rapid amplification of cDNA end(RACE)and reverse transcription- polymerase chain reaction(RT-PCR).The length of cGnRH-Ⅱ cDNA1 and cDNA2 was 622 and 578 base pairs(bp),respectively.The cGnRH-Ⅱ precursors encoded by two cDNAs consisted of 86 amino acids,including a signal peptide,cGnRH-Ⅱ decapeptide and a GnRH-associated peptide(GAP)linked by a Gly-Lys-Arg proteolytic site.The results of intron trapping and Southern blot showed that two differing cGnRH-Ⅱ genes in common carp genome were further identified,and that two genes might exist as a single copy.The multi-gene coding of common carp cGnRH-Ⅱ gene offered novel evidence for gene duplication hypothesis.Using semi-quantitative RT-PCR,expression and relative expression levels of cGnRH-Ⅱ genes were detected in five dissected brain regions,pituitary and gonad of common carp.With the exception of no mRNA2 in ovary,two cGnRH-Ⅱ genes could be expressed in all the detected tissues.However,expression levels showed an apparent difference in different brain regions,pituitary and gonad.According to the expression characterization of cGnRH-Ⅱ genes in brain areas,it was presumed that cGnRH-Ⅱ might mainly work as the neurotransmitter and neuromodulator and also operate in the regulation for the GnRH releasing.Then,the expression of cGnRH-Ⅱ genes in pituitary and gonad suggested that cGnRH-Ⅱ might act as the autocrine or paracrine regulator.

  15. Comparaison de populations de carpe commune (Cyprinus carpio à taille commerciale : intérêt d'une approche globale

    Directory of Open Access Journals (Sweden)

    PEREIRA V.

    1999-07-01

    Une discrimination géographique des populations est possible mais elle repose sur une approche globale intégrant l'ensemble des paramètres. Les différences semblent essentiellement liées aux conditions environnementales et permettent de distinguer les lots issus des régions où l'élevage est plus intensif et traditionnellement centré sur la carpe (Dombes, Forez, Lorraine et les lots correspondant à un élevage plus extensif et davantage orienté vers le marché du repeuplement (Allier, Brenne, Poitou-Charentes.

  16. The influence of stocking density on the growth of common carp, Cyprinus carpio, in a recirculating aquaculture system

    OpenAIRE

    Ionica Enache; Victor Cristea; Tudor Ionescu; Săndita Ion

    2011-01-01

    The paper presents some aspects regarding the influence of stocking density on the breeding of the common carp in a recirculating aquaculture system (RAS). The experiment covered a 30 dayperiod. It was conducted in four aquaculture tanks, 500 L/tank, in the recirculating aquaculture pilot system of “Dunarea de Jos” University of Galați. Two kind of stocking density variants were compared: 64 kg/m3 in V1 (B1, B3) and 32 kg/m3 in V2 (B2, B4). The stocking density was: 491 fish in B1, avera...

  17. Molecular cloning, immunohistochemical localization, characterization and expression analysis of caspase-9 from the purse red common carp (Cyprinus carpio) exposed to cadmium

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Dian; Xu, Zhen’e [Medical College of Nanchang University, Nanchang 330006 (China); Institute of Immunotherapy, Nanchang University, Nanchang 330006 (China); Zhang, Xiaoyan [Medical College of Nanchang University, Nanchang 330006 (China); Wang, Hongmei [Medical College of Nanchang University, Nanchang 330006 (China); Institute of Immunotherapy, Nanchang University, Nanchang 330006 (China); Wang, Yannan [Medical College of Nanchang University, Nanchang 330006 (China); Min, Weiping, E-mail: weiping.min@gmail.com [Medical College of Nanchang University, Nanchang 330006 (China); Institute of Immunotherapy, Nanchang University, Nanchang 330006 (China); Jiangxi Academy of Medical Sciences, Nanchang 330006 (China)

    2013-10-15

    Highlights: •The cDNA of caspase-9 in common carp was cloned. •The evolutionary conservation including caspase recruitment domain, large and small subunits was clarified. •The mRNA level of caspase-9 cannot be used as a major marker at an earlier point in the apoptotic cascade. •Caspase-9 cleavage form was detected. •Immunopositive staining was limited to the cytoplasm of renal tubular epithelial cells. -- Abstract: Caspase-9, the essential initiator caspase is believed to play a central role in mitochondria-mediated apoptosis signaling. In this study, we isolated the caspase-9 gene from common carp, one of the most important industrial aquatic animals in China using rapid amplification of cDNA ends (RACE). The deduced amino acid sequence of caspase-9, composed of 436 amino acids, showed approximately 47.6% identity and 64.7% similarity to human caspase-9. It also possessed a conserved caspase-associated recruitment domain (CARD), a large subunit and a small subunit. Phylogenetic analysis clearly demonstrated that caspase-9 formed a clade with cyprinid fish caspase-9. Real-time quantitative PCR analysis revealed that caspase-9 transcripts were not significantly increased in kidney after exposure to cadmium (Cd). Whereas caspase-9 cleaved fragments were detected using Western blot analysis with the same Cd treatment condition. Furthermore, the result of immunohistochemical detection showed immunoreactivities were predominantly limited to the cytoplasm of renal tubular epithelial cells and no remarkable changes of immunopositive staining were observed after Cd treatment. Accordingly, the results signify that caspase-9 may play an essential role in Cd induced apoptosis.

  18. Molecular cloning, immunohistochemical localization, characterization and expression analysis of caspase-9 from the purse red common carp (Cyprinus carpio) exposed to cadmium

    International Nuclear Information System (INIS)

    Highlights: •The cDNA of caspase-9 in common carp was cloned. •The evolutionary conservation including caspase recruitment domain, large and small subunits was clarified. •The mRNA level of caspase-9 cannot be used as a major marker at an earlier point in the apoptotic cascade. •Caspase-9 cleavage form was detected. •Immunopositive staining was limited to the cytoplasm of renal tubular epithelial cells. -- Abstract: Caspase-9, the essential initiator caspase is believed to play a central role in mitochondria-mediated apoptosis signaling. In this study, we isolated the caspase-9 gene from common carp, one of the most important industrial aquatic animals in China using rapid amplification of cDNA ends (RACE). The deduced amino acid sequence of caspase-9, composed of 436 amino acids, showed approximately 47.6% identity and 64.7% similarity to human caspase-9. It also possessed a conserved caspase-associated recruitment domain (CARD), a large subunit and a small subunit. Phylogenetic analysis clearly demonstrated that caspase-9 formed a clade with cyprinid fish caspase-9. Real-time quantitative PCR analysis revealed that caspase-9 transcripts were not significantly increased in kidney after exposure to cadmium (Cd). Whereas caspase-9 cleaved fragments were detected using Western blot analysis with the same Cd treatment condition. Furthermore, the result of immunohistochemical detection showed immunoreactivities were predominantly limited to the cytoplasm of renal tubular epithelial cells and no remarkable changes of immunopositive staining were observed after Cd treatment. Accordingly, the results signify that caspase-9 may play an essential role in Cd induced apoptosis

  19. 类胡萝卜素在锦鲤组织器官中的沉积与分布%Deposition and distribution of carotenoids in different tissues of ornamental carp Cyprinus carpio

    Institute of Scientific and Technical Information of China (English)

    姜志强; 崔培; 秦强; 刘芳; 高小强; 田青杰; 周小龙

    2012-01-01

    在6个90L蓝色方形水槽中各放养22尾锦鲤Cyprinus carpio幼鱼,初始体质量为(7.31±0.12)g,在水温相同的环境条件下先用添加虾青素(130 mg/kg)和螺旋藻(质量分数为12%)2组饲料饲喂21 d,再用没有添加虾青素和螺旋藻的基础饲料饲喂20 d,每个试验组设3个平行,研究了虾青素和螺旋藻对锦鲤各组织器官中类胡萝卜素含量的影响以及在锦鲤各组织器官中的代谢途径.结果表明:投喂添加着色剂的饲料后,锦鲤各组织器官中的类胡萝卜素含量均随投喂时间的延长而升高;类胡萝卜素在锦鲤各组织器官中的含量从高至低依次为皮肤>鳍>肝胰脏>眼>肌肉,皮肤和鱼鳍是锦鲤色素沉积的主要部位;锦鲤的眼、肌肉、肝胰脏中色素的沉积随着饲养时间的延长变化不显著,而锦鲤的背鳍、尾鳍和皮肤随饲养时间的延长变化较显著;停喂添加着色剂的饲料后,锦鲤各组织器官中的类胡萝卜素含量均随停喂时间的延长而降低.以虾青素作为着色剂时,锦鲤体表类胡萝卜素含量于饲喂第9天开始显著升高;而以螺旋藻为着色剂时,锦鲤体表类胡萝卜素含量于饲喂第13天开始显著升高,锦鲤对虾青素的沉积速度比螺旋藻快.%A feeding trial was conducted to investigate the effects of dietary astaxanthin and Spirulina on the carote noids contents and the metabolic pathways in different tissues of ornmmental carp Cyprinus carpio with initial body weight of (7.31±0.12) g. The fish were stocked into 90 L blue plastic tanks at a rate of 22 individuals/tank and fed the diets containing astaxanthin (130 mg/kg) and Spirulina( 12% ) for 21 days, and then fed the diet without astaxanthin and Spirulina for 20 days. The results showed that the carotenoid contents in different tissues of the fish were found to increase with increase in feeding period. The total deposition of the carotenoid content was mainly concentrated in

  20. 鲤病原鳗利斯顿氏菌的分离鉴定及生物学特性研究%Study on biological characteristics of pathogenic Listonella anguillarum isolated from carp Cyprinus carpio L.

    Institute of Scientific and Technical Information of China (English)

    张晓君; 阎斌伦; 邴旭文; 毕可然; 秦蕾; 秦国民

    2009-01-01

    Dominant bacteria were isolated from diseased carp, and have strong pathogenicity to carp by artificial challenge. The biological characteristics were examined, including morphological, physiological and biochemical characteristics; the 16S rRNA and gyrB gene were partially sequenced and compared with sequences deposited in databases, then the molecular phylogenetic trees were constructed. The sequenced 16S rRNA gene of isolate (GenBank accession No. FJ824662) was 1449 bp in length, the sequenced gyrB gene of isolate (GenBank accession No.GQ452957) was 1202 bp in length, and two genes all exhibited high similarity (over 97%) with the 16S rRNA and gyrB gene of Listonella anguilla-rum from GenBank database, respectively. Detection of the activity of extracellulase and hemolysin showed that the isolate could produced diastase, proteinase, DNase, lecithinase and haemolysin, but no lipase, with p-haemolysis in containing 7% defibrinated rabbit blood agar plates, and 4 isolates could be amplified hemolysin gene and metalloprotease gene. The results revealed that the identified strains belonged to Languillnrum of Listonella ( MacDonell and Colwell 1986) based on their phenotypic and molecular characteristics. Drug resistance of the isolate to 49 antimicrobial agents were detected, the results showed that isolates were resistant to 12 agents including penicillin G; were sensitive to 5 agents including clindamycin; were high sensitive to 32 agents including enoxacin.%从患病鲤(Cyprinus carpio L.)体内分离到一株优势生长菌,人工感染试验证明该菌对鲤有较强的致病性.对分离菌进行了形态特征、理化特性等生物学性状检验;测定了分离菌的16S rRNA和gyrB基因的部分序列,并与相关细菌16S rRNA和gyrB基因序列进行比对后,构建了基于两种基因的系统发生树.结果显示:分离菌所扩增的16S rRNA和gyrB基因序列与GenBank数据库中鳗利斯顿氏菌的16S rRNA和gyrB基因序列相似性均在97%

  1. Molecular cloning of the obese gene from Cyprinus carpio and its expression in Escherichia coli

    Institute of Scientific and Technical Information of China (English)

    Dai Hanchuan; Long Liangqi; Ding Guang

    2006-01-01

    Aiming to analyze the characteristics of the Cyprinus carpio obese gene structure and the biological activity of its expression product,we amplified the carp obese gene using reverse transcription-polymerase chain reaction from carp mesentery adipose tissue RNA.Sequence analysis revealed that it has a length of 438 nt,which encodes a 146-amino acid peptide.When nucleotide sequence and deduced amino acid sequence were compared with homologous sequences from those of humans,pigs,and rats,they displayed a fairly high degree of conservation (the homology of the nucleotide sequence was 84%,86%,and 95%,respectively,while that of the amino acid sequence was 84%,82%,and 96%,respectively,for humans,pigs,and rats).The cDNA fragment was inserted into the expression vector pET-28a,and the resulting plasmid was expressed in Escherichia coli BL21 (DE3) by isopropylthiogalactoside induction.Resuits of sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that a fusion protein was specifically expressed in E.coli BL21 (DE3).The weight of the fusion protein was about 20 kDa,and a 16-kDa protein was expressed from the carp obese gene.By gel thin-layer scanning analysis,the amount of target protein was determined to be about 20%.The purified product was found to be biologically active and to reduce the food intake and body weight of mice during tests.

  2. Manejo de microembalses para el cultivo extensivo de carpa común (Cyprinus carpio Linnaeus, 1758 en la región de Zacapu, Michoacán, México Water basins management for extensive aquaculture of common carp (Cyprinus carpio Linnaeus, 1758 in the region of Zacapu, Michoacan, Mexico

    Directory of Open Access Journals (Sweden)

    Ana Bel Huipe-Ramos

    2009-08-01

    Full Text Available El presente estudio aporta, mediante pruebas experimentales, una estimación de la capacidad de carga de los microembalses de la región de Zacapu, Michoacán para ser utilizada en la acuicultura extensiva y semintensiva de carpa común (Cyprinus carpió. Un análisis factorial (3 × 3 con tres densidades (D B = 0.5 org. · m-2, D M = 1 org. · m-2 y D A = 2 orgs. · m-2 y tres regímenes de alimentación (R0= sin fertilizar o con el alimento natural, R F= con fertilización y R+= suplementada con subproductos agrícolas incluyó nueve tratamientos combinados entre densidades y regímenes alimenticios con tres réplicas en cada caso. La combinación de baja densidad y alimentación natural, análoga a una acuicultura extensiva en microembalses, alcanzó un rendimiento máximo equivalente a 5,000 orgs.· ha-1 . Pruebas empíricas realizadas a la par en siete microembalses temporales (This study provides an assessment of the carrying capacity of water bodies in the area of Zacapu, Michoacan for extensive and semintensive aquaculture with carp (Cyprinus carpió by means of experimental trials in 27 ponds. A 3 × 3 factorial analysis with three densities (D B = 0.5 org. · m-2, D M=1 org. · m-2 and D A= 2 orgs. · m-2 and three feeding regimes (R0 = without fertilization, R F = fertilization and R+ = farming by-products included nine combined treatments between densities and feeding regimes with three replicates for each case. The combination of low density and natural food, analog to an extensive aquaculture in small water reservoirs, attained a maximum yield equivalent to 5,000 orgs.· ha-1 . Empirical trials accomplished at seven temporary basins (<10 ha under conditions of different stocking densities from 1,500 to 6,000 orgs.· ha-1 lead to fairly similar yields to those obtained in the ponds. Thus, the general index used in Mexico for extensive aquaculture of 1 org. · m² overloads the regional temporary ecosystems and only the possibility

  3. Optimization of the isolation and cultivation of Cyprinus carpio primary hepatocytes

    OpenAIRE

    Yanhong, Fan; Chenghua, He; Guofang, Liu; Haibin, Zhang

    2008-01-01

    The aquatic environment is affected by numerous chemical contaminants. There is an increasing need to identify these chemicals and to evaluate their potential toxicity towards aquatic life. In this research we optimized techniques for primary cell culture of Cyprinus carpio hepatocytes as one adjunct model for ecotoxicological evaluation of the potential hazards of xenobiotics in the aquatic environment. In this study, Cyprinus carpio hepatocytes were isolated by mechanical separation, two-st...

  4. Common Carp Abundance, Biomass, and Removal from Dewey and Clear Lakes on the Valentine National Wildlife Refuge: Does Trapping and Removing Carp Payoff?

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Common carp Cyprinus carpio is a nonnative invasive nuisance species to North America. Many authors have documented the detrimental affects of common carp invasions...

  5. Vitellogenin induction in Cyprinus carpio treated with 17 {beta} Estradiol and 4-Nonylphenol; Induzione della vitellogenina in esemplari di Cyprinus carpio trattati sperimentalmetne con 17 {beta} Estradiolo e 4-Nonilfenolo

    Energy Technology Data Exchange (ETDEWEB)

    Casini, S. [Siena Univ., Siena (Italy). Dipt. di Scienze Ambientali; Fossi, M. C. [Messina Univ., Messina (Italy). Dipt. di Biologia Animale ed Ecologia Marina; Mori, G. [Siena Univ., Siena (Italy). Dipt. di Scienze Ambientali; Bjornstad, A. [Rogaland Research, Stavanger (Norway)

    2001-01-01

    A major problem of freshwater environments is the massive discharge of surfactants such as alkylphenols that have been shown to have estrogenic activity. One marker of the effects of estrogenic compounds is induction of vitellogenin (Vtg), a yolk protein precursor of lower viviparous vertebrates. The aim of the present study was to validate induction of Vtg in the common carp (Cyprinus carpio) as a biomarker for monitoring freshwater ecosystems. Sexually undifferentiated specimens of common carp were treated experimentally with 17 {beta},-Estradiol and increasing doses of 4-Nonylphenol and levels plasma Vtg were measured in order to: 1) validate an E.L.I.S.A. assay for plasma Vtg in the common carp using the polyclonal rabbit anti-salmon Vtg antibody; 2) check the sensitivity of carp juveniles in producing Vtg in response to estrogen stimulation. [Italian] Uno dei maggiori problemi legati alla salute degli ecosistemi acquatici e' la presenza di composti di sintesi con attivita' estrogenica quali, ad esempio, gli alchilfenoli polietossilati. Tra i piu' interessanti strumenti di indagine capaci di individuare gli effetti di tali composti sulle comunita' naturali c'e' l'induzione, nei vertebrati ovipari, della vitellogenina (Vtg; proteina precursore delle proteine del tuorlo) un importante biomarker specifico di effetto per composti estrogenici. Lo scopo del presente studio e' stato quello di validare l'induzione della Vtg nella carpa comune (Cyprinus carpio) come biomarker per il monitoraggio degli ecosistemi acquatici interni. Esemplari sessualmente immaturi di carpa comune sono stati trattati sperimentalmente con 17 {beta}, -Estradiolo e dosi crescenti di 4-Nonilfenolo al fine di: 1) mettere a punto il test E.L.I.S.A. per la valutazione dei livelli plasmatici di Vtg nella carpa utilizzando l'anticopro policlonale Rabbit anti Salmon Vtg; 2) verificare le capacita' di risposta a stimoli estrogenici nella specie

  6. Mass mortality in ornamental fish, Cyprinus carpio koi caused by a bacterial pathogen, Proteus hauseri.

    Science.gov (United States)

    Kumar, Raj; Swaminathan, T Raja; Kumar, Rahul G; Dharmaratnam, Arathi; Basheer, V S; Jena, J K

    2015-09-01

    Moribund koi carp, Cyprinus carpio koi, from a farm with 50% cumulative mortality were sampled with the aim of isolating and detecting the causative agent. Three bacterial species viz., Citrobacter freundii (NSCF-1), Klebsiella pneumoniae (NSKP-1) and Proteus hauseri [genomospecies 3 of Proteus vulgaris Bio group 3] (NSPH-1) were isolated, identified and characterized on the basis of biochemical tests and sequencing of the 16S rDNA gene using universal bacterial primers. Challenge experiments with these isolates using healthy koi carp showed that P. hauseri induced identical clinical and pathological states within 3 d of intramuscular injection. The results suggest P. hauseri (NSPH-1) was the causative agent. In phylogenetic analysis, strain NSPH-1 formed a distinct cluster with other P. hauseri reference strains with ≥99% sequence similarity. P. hauseri isolates were found sensitive to Ampicillin, Cefalexin, Ciprofloxacin and Cefixime and resistant to Gentamycin, Oxytetracycline, Chloramphenicol, and Kanamycin. The affected fish recovered from the infection after ciprofloxacin treatment. PMID:26028178

  7. Genetic diversity of Cyprinus carpio from Ohrid Lake, estimated by four microsatellite loci

    Directory of Open Access Journals (Sweden)

    ADIOLA BIBA

    2014-06-01

    Full Text Available Common carp (Cyprinus carpio L is an important fish species of Ohrid lake. The aim of the present study was to evaluate the genetic diversity of common carp using microsatellite markers. A total of 30 individuals were genotyped for 4 microsatellite loci (MFW1, MFW6, MFW7, MFW18. All the microsatellite loci were polymorphic. A total of 84 alleles were distinguished. The allelic number varied from 19 to 23 with mean allelic number of 21. The effective number of alleles varied from 9.92 to 11.84 with a mean of 11.66. The observed heterozygosity ranged from 0.32 to 0.73 with mean value of 0.57. The loci were highly informative. The PIC values ranged from 0.89 to 0.91 with mean of 0.90. The values of Shannon information index (I ranges from 2.63 to 2.78. The population was not in Hardy-Weinberg equilibrium (HWE for all of four loci. The mean expected heterosygosity value (0.91 was higher the mean observed heterosygosity (0.57. The mean fixation index (F over the loci was 0.37. A bottleneck analysis is carried out, which shows no recent bottleneck. Factorial Correspondence Analysis (FCA shows a close relationship between individuals.

  8. Toxicity of uranium and plutonium to the developing embryos of fish. [Cyprinus carpio, Pimephales promelas

    Energy Technology Data Exchange (ETDEWEB)

    Till, J.E.; Kaye, S.V.; Trabalka, J.R.

    1976-07-01

    The radiological and chemical toxicity of plutonium and uranium to the developing embryos of fish was investigated using eggs from carp, Cyprinus carpio, and fathead minnows, Pimephales promelas. Freshly fertilized eggs were developed in solutions containing high specific activity /sup 238/Pu or /sup 232/U or low specific activity /sup 244/Pu, /sup 235/U, or /sup 238/U. Quantitative tests to determine the penetration of these elements through the chorion indicated that plutonium accumulated in the contents of carp eggs reaching a maximum concentration factor of approximately 3.0 at hatching. Autoradiographs of 16 ..mu.. egg sections showed that plutonium was uniformly distributed in the egg volume. Uranium localized in the yolk material, and the concentration factor in the yolk sac remained constant during development at approximately 3.3. Doses from /sup 238/Pu which affected hatchability of the eggs were estimated to be 1.6 x 10/sup 4/ rads and 9.7 x 10/sup 3/ rads for C. carpio and P. promelas, respectively; doses from /sup 232/U were 1.3 x 10/sup 4/ rads for C. carpio and 2.7 x 10/sup 3/ rads for P. promelas. A greater number of abnormal larvae than in control groups was produced by /sup 238/Pu doses of 4.3 x 10/sup 3/ rads to carp and 5.7 x 10/sup 2/ rads to fathead minnows; 3.2 x 10/sup 3/ rads and 2.7 x 10/sup 2/ rads were estimated from /sup 232/U. Eggs that were incubated in 20 ppM /sup 244/Pu did not hatch. This mortality may have been the result of chemical toxicity of plutonium. Concentrations of 60 ppM of /sup 235/U and /sup 238/U did not affect egg hatching. Based on these data, concentrations in fish eggs were calculated for representative concentrations of uranium and plutonium in natural waters and the corresponding dose levels are below those levels at which observable effects begin to occur.

  9. Toxicological effects of ammonia on gills of Cyprinus carpio var. communis (Linn.)

    Institute of Scientific and Technical Information of China (English)

    Senthamilselvan Devaraj; Chezhian Arulprakasam; Arul Pandian Kandhan; Kabilan Neelamegam; Rajalakshmi Kalaiselvan

    2014-01-01

    Objective:To monitor the response of gills of Cyprinus carpio var. communis to LC50 levels of ammonia over a period of 96 h by bath exposure, using scanning electron microscopy (SEM). Methods: Cyprinus carpio procured from a local fish farm in Vadalore, Cuddalore District, Tamil Nadu, India were acclimatized under laboratory conditions for 20 d. Fish were placed into experimental tanks with 20 L of water and 22 mg/L ammonia. A total of 10 fish were collected from each experimental tank every 24 h for histopathological studies. Results:Histopathological studies showed these changes in the gills:secondary lamellar fusion, haemorrhage, oedema, epithelial hyperplasia, and chloride cell proliferation. Occasionally, multifocal necrosis of inter-lamellar regions of gill filaments but with no apparent haemorrhage was observed under electron microscopy. Conclusion:The present study shows that histopathological and ultrastructural alterations in gills are useful indicators for ammonia toxicity in Cyprinus carpio.

  10. Evaluation of gamma radiation induced genetic damage in the fish Cyprinus carpio using comet assay

    International Nuclear Information System (INIS)

    Radionuclides released from various sources including the industries, as well as, accidental release during a nuclear disaster can contaminate inland water bodies. Suitable bio-monitoring methods/biomarkers are the need of the day to assess the impact of high/low levels of radiation exposure in aquatic environment. Fishes are very important as a group of ecologically and commercially important non-human biota and are often used as a bioindicators of aquatic pollution. Present work was carried out to assess the genotoxic effect of gamma radiation on fresh water fish Cyprinus carpio (common carp) in vivo using comet assay. Fishes were irradiated with 2, 4, 6, 8 and 10 Gy of gamma rays using a teletherapy machine and comet assay was performed on nucleated erythrocytes after 24, 48 and 72 h of irradiation . A significant increase in % tail DNA was observed at all the doses of gamma radiation as compared to controls indicating radiation induced DNA damage in a dose-dependent manner. Maximum % tail DNA was observed at 24 h which gradually declined till 72 h, in a time-dependent manner. This decrease in damage may indicate repair of the damaged DNA and or loss of heavily damaged cells, over a period of time. The study reveals that the comet assay may be used as a sensitive and rapid method to detect genotoxicity of gamma radiation and other environmental pollutants in sentinel species. (author)

  11. Assessment of the toxicity of waste water from a textile industry to Cyprinus carpio.

    Science.gov (United States)

    Roopadevi, H; Somashekar, R K

    2012-03-01

    Static, short-term, acute toxicity tests were performed over a period of 96 hrs using different concentrations of influent and effluent of textile industry waste water with the objective of evaluating their acute toxicity on fresh water fish, Cyprinus carpio (common carp). The LC50 24, 48, 72 and 96 hr of influent and effluent were 25.9, 21.10, 15.66, 11.11% (v/v) and 63.18, 54.89, 48.62, 36.04% (v/v), respectively. The acute toxic unit TUa values for 24, 48, 72, 96 hr for influent and effluent are 3.85, 4.73, 6.38, 8.99 and 1.58, 1.82, 2.05, 2.77, respectively. Correspondingly, the TF was found to be 1, 1.22, 1.65 and 2.33 for influent, and for effluent 1, 1.15, 1.29 and 1.75. Total efficiency of the treatment was 69.16% and the safe concentration of effluent is set to be 3.60%. These data are highly useful in establishing limits of acceptability by the aquatic animals. The need to introduce toxicity evaluation assay for confirming the quality of effluent from the point view of effective environmental safe limits and to ensure integrity of aquatic environment, is stressed. PMID:23033675

  12. Detection of micronuclei in peripheral erythrocytes of Cyprinus carpio exposed to metallic mercury.

    Science.gov (United States)

    Nepomuceno, J C; Ferrari, I; Spanó, M A; Centeno, A J

    1997-01-01

    Cyprinus carpio fish (carp), exposed to elemental or metallic mercury (Hg0) at concentrations of 2.0, 20.0, and 200.0 mg per liter of water, were kept in concrete tanks for 159 days. Ten fish were used for each concentration level. Thirteen samples of peripheral blood were collected from each animal through gill puncture, 12 during the first 90 days of the experiment, and the last one at the end of the experiment. The micronucleus test (MNT) was designed to study dose and time yield effects of mercury after indirect exposure in vivo. The results indicated that for a concentration of 2.0 mg Hg0/l, there was no significant increase in frequency of micronuclei (MN), but at higher concentrations (20.0 and 200.0 mg Hg0/l) there was a significant increase in MN frequencies. This effect was higher after 31 days of exposure, followed by slight stabilization and gradual decrease. PMID:9366907

  13. RESPONSE OF CYPRINUS CARPIO TO PHENOL AND FURFURAL SHOCK LOADS IN PACT SYSTEM

    Directory of Open Access Journals (Sweden)

    A. Mesdaghinia

    1999-08-01

    Full Text Available Treatment efficiency, design factors and kinetic coefficients were studied using two pilots of activated sludge (AS and powdered activated carbon technology (PACT, for treating Tehran Oil Refinery effluent, in a duration of more than 12 months In order to evaluate the performance of each system against growth inhibitor shock loads, different concentrations of phenol and furfural (10-300 mg/l were applied, following a series of experiments in which, treated effluents from the two systems were used as influent water into two aquariums containing freshwater Common Carp (Cyprinus carpio of 9-15g weight, 6-9 cm length and with a fish density of 5g/1. Results of fish fatal rates with 125 and 250-mg/l phenol were 16% and 38% for AS system and 5.6% and 8% for PACT system, respectively. Changing the growth inhibitor to furfural, with a concentration of 100 mg/I, led to the fatal rates of 100% and 0% for AS and PACT systems, respectively. Increasing furfural load to 300 mg/I destroyed only 20% of fish in PACT pilot.

  14. PEMBERIAN TOTAL FENOL TERIPANG PASIR (Holothuria scabra UNTUK MENINGKATKAN LEUKOSIT DAN DIFERENSIAL LEUKOSIT IKAN MAS (Cyprinus carpio YANG DIINFEKSI BAKTERI Aeromonas Hydrophila

    Directory of Open Access Journals (Sweden)

    Achmad Suhermanto

    2011-10-01

    Full Text Available Freshwater fishery commodities are most likely to be increased production of carp (Cyprinus carpio. Intensification of cultivation which raises new problems with fish disease outbreaks caused by Aeromonas hydrophila. Providing of immunostimulatory bioactive compounds of sea cucumbers (Holothuria scabra can enhance non-specific immune responses in the goldfish as a mechanism of defense against disease. The purpose of this study is to determine the role and optimal dose of total phenols sea cucumber in enhancing non-specific immune response seen from the hematology parameters Extraction of bioactive components from sea cucumber prepared by using methanol and fractionated with ethyl acetate solvent (v/v. Identification of total phenols in ethyl acetate fraction performed using UV-Vis spectrophotometer and infrared. Total phenol produced was tested on carp with intraperitonial injection, the concentration of 0.09, 0.18 and 0.27 mg phenol/kg fish. Challenge test was done by using the Aeromonas hydrophila (107 cells/ml through the immersion method. The observations included hematological parameters and total plasma proteins.The observations of hematological parameters that Leukocytes, Neutrophils pre-and post-infection were significantly increased. Lymphocyte pre infection was increased significantly and post-bacterial infection was decreased significantly. Eosinophils, Monocytes pre-and post-bacterial infection were not differ significantly between treatment. The use of phenolic compounds at 0.09 mg / kg can be increase of non-specific immune response in carp (Cyprinus carpio. Key Words : Total phenol, Aeromonas hydrophila, Cyprinus carpio, nonspecific immune response 

  15. 3×3完全双列杂交F1不同阶段生长特点的分析%CHARACTERS OF DIFFERENT GROWTH STAGE ON THE F1 PROGENY OF 3×3 FULL DIALLEL CROSS IN COMMON CARP (CYPRINUS CARPIO L.)

    Institute of Scientific and Technical Information of China (English)

    苏胜彦; 董在杰; 袁新华; 徐跑; 马庆男; 张建桥; 刘伟; 马良骁

    2012-01-01

    为了了解鲤杂交F1不同组合、不同阶段生长性状的变化情况,研究以建鲤、黄河鲤和黑龙江野鲤3个鲤品种双列杂交F1为试验材料,通过组合、组合内性别间、性别、不同时期体重、体增重以及协方差分量的分析,来确定完全双列杂交F1生长性状的变化特点,以及在此过程中起重要作用的影响因素.结果表明:不同时期各个组合体重不同,不同时期组合内性别间体重差异不同;不同时期不同性别间体重差异不同;不同协方差分量,同一组合PIT标记17个月时的体重的最小二乘估计值不同,同一个协方差分量,9个组合中极值估计值出现的组合也不同;除PIT标记17个月后的体厚作为协方差分量外,性别之间体重没有差异,其余分量均是雌鱼体重显著高于雄鱼体重.这些说明选取合适的协方差分量对组合的选择和育种的结果有重要影响.%Jian carp (Cyprinus carpio var.jian) examined and approved in 1990, was the first approved aquatic specie using integrated genetic breeding technology. Jian carp is a good stock of cultivated fish species which has good growth traits and good disease-resisitant performance. Although Jian carp has been disseminated wildly, there still has a great need to enhance fine growth performance through continuous selection and genetic improvement. Huanghe carp is a remarkable local specie of common carp in northern China, which possesses good meat quality, strong cold resistance and high efficiency of food conversion. Heilongjiang carp has high frigid resistance, good quality and growth trait. Specific hybridization is an effective pathway for growth improvement in common carp breeding programs. In most cases, obvious heterosis may be observed in hybrids in growth rate, compared to their parents, suggesting a potential resolution to current problems. Generally, the growth pattern of hybrids and their parental species usually reflect that the hybrids were

  16. Effects of different inorganic arsenic species in Cyprinus carpio (Cyprinidae) tissues after short-time exposure: Bioaccumulation, biotransformation and biological responses

    Energy Technology Data Exchange (ETDEWEB)

    Ventura-Lima, Juliane [Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS (Brazil); Programa de Pos-Graduacao em Ciencias Fisiologicas - Fisiologia Animal Comparada (FURG), Rio Grande, RS (Brazil); Fattorini, Daniele; Regoli, Francesco [Istituto di Biologia e Genetica, Universita Politecnica delle Marche, 60100, Ancona (Italy); Monserrat, Jose M., E-mail: josemmonserrat@pesquisador.cnpq.b [Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS (Brazil); Programa de Pos-Graduacao em Ciencias Fisiologicas - Fisiologia Animal Comparada (FURG), Rio Grande, RS (Brazil)

    2009-12-15

    Differences in the toxicological and metabolic pathway of inorganic arsenic compounds are largely unknown for aquatic species. In the present study the effects of short-time and acute exposure to As{sup III} and As{sup V} were investigated in gills and liver of the common carp, Cyprinus carpio (Cyprinidae), measuring accumulation and chemical speciation of arsenic, and the activity of glutathione-S-transferase omega (GST OMEGA), the rate limiting enzyme in biotransformation of inorganic arsenic. Oxidative biomarkers included antioxidant defenses (total glutathione-S-transferases, glutathione reductase, glutathione, and glucose-6-phosphate dehydrogenase), total scavenging capacity toward peroxyl radicals, reactive oxygen species (ROS) measurement and lipid peroxidation products. A marked accumulation of arsenic was observed only in gills of carps exposed to 1000 ppb As{sup V}. Also in gills, antioxidant responses were mostly modulated through a significant induction of glucose-6-phosphate dehydrogenase activity which probably contributed to reduce ROS formation; however this increase was not sufficient to prevent lipid peroxidation. No changes in metal content were measured in liver of exposed carps, characterized by lower activity of GST OMEGA compared to gills. On the other hand, glutathione metabolism was more sensitive in liver tissue, where a significant inhibition of glutathione reductase was concomitant with increased levels of glutathione and higher total antioxidant capacity toward peroxyl radicals, thus preventing lipid peroxidation and ROS production. The overall results of this study indicated that exposure of C. carpio to As{sup III} and As{sup V} can induce different responses in gills and liver of this aquatic organism. - Common carp (Cyprinus carpio) presented marked differences between gills and liver after arsenic exposure in terms of antioxidant responses and also in biotransformation.

  17. Effects of different inorganic arsenic species in Cyprinus carpio (Cyprinidae) tissues after short-time exposure: Bioaccumulation, biotransformation and biological responses

    International Nuclear Information System (INIS)

    Differences in the toxicological and metabolic pathway of inorganic arsenic compounds are largely unknown for aquatic species. In the present study the effects of short-time and acute exposure to AsIII and AsV were investigated in gills and liver of the common carp, Cyprinus carpio (Cyprinidae), measuring accumulation and chemical speciation of arsenic, and the activity of glutathione-S-transferase omega (GST Ω), the rate limiting enzyme in biotransformation of inorganic arsenic. Oxidative biomarkers included antioxidant defenses (total glutathione-S-transferases, glutathione reductase, glutathione, and glucose-6-phosphate dehydrogenase), total scavenging capacity toward peroxyl radicals, reactive oxygen species (ROS) measurement and lipid peroxidation products. A marked accumulation of arsenic was observed only in gills of carps exposed to 1000 ppb AsV. Also in gills, antioxidant responses were mostly modulated through a significant induction of glucose-6-phosphate dehydrogenase activity which probably contributed to reduce ROS formation; however this increase was not sufficient to prevent lipid peroxidation. No changes in metal content were measured in liver of exposed carps, characterized by lower activity of GST Ω compared to gills. On the other hand, glutathione metabolism was more sensitive in liver tissue, where a significant inhibition of glutathione reductase was concomitant with increased levels of glutathione and higher total antioxidant capacity toward peroxyl radicals, thus preventing lipid peroxidation and ROS production. The overall results of this study indicated that exposure of C. carpio to AsIII and AsV can induce different responses in gills and liver of this aquatic organism. - Common carp (Cyprinus carpio) presented marked differences between gills and liver after arsenic exposure in terms of antioxidant responses and also in biotransformation.

  18. Malheur - Common Carp Movement Control

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Invasive common carp Cyprinus carpio were introduced into the Harney Basin in the 1920’s and were recognized as a problem in Malheur Lake in 1952. The common carp...

  19. Monoclonal antibodies against goldfish (Carassius auratus) immunoglobulin: application to the quantification of immunoglobulin and antibody-secreting cells by ELISPOT and seric immunoglobulin and antibody levels by ELISA in carp (Cyprinus carpio).

    Science.gov (United States)

    Siwicki, A K; Vergnet, C; Charlemagne, J; Dunier, M

    1994-01-01

    Monoclonal antibodies (mAbs) raised against heavy and light chains of goldfish immunoglobulin (Ig) were characterized by a Western blot technique. A complete cross-reactivity was observed between carp and goldfish Ig. These mAbs were used for the quantification of carp Ig and anti-Yersinia ruckeri antibodies by ELISA. An ELISPOT assay was also developed in carp to quantify Ig-secreting cells (ISC) and antibody-secreting cells (ASC). The number of ASC was maximum on day 18 post-vaccination and decreased to the basal level on day 28. The antibody levels in sera were maximum on day 18 and slowly decreased until day 28. PMID:7951348

  20. Comparative study of ß-glucan induced respiratory burst measured by nitroblue tetrazolium assay and real-time luminol-enhanced chemiluminescence assay in common carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Jiménez, Natalia Ivonne Vera; Pietretti, D.; Wiegertjes, G. F.; Nielsen, Michael Engelbrecht

    2013-01-01

    clearance of pathogens and tissue regeneration processes. Therefore, the respiratory burst and associated ROS constitute important indicators of fish health status. This paper compares two methods for quantitation of ROS produced during the respiratory burst in common carp: the widely used, single...

  1. Dimethoate Induced Behavioural Changes in Juveniles of Cyprinus carpio var. communis under Temperate Conditions of Kashmir, India

    Science.gov (United States)

    Shah, Feroz A.; Mukhtar, Malik; Balkhi, Masood H.; Bhat, Farooz A.; Bhat, Bilal A.

    2016-01-01

    The present study was designed to investigate acute toxicity of dimethoate on juvenile Cyprinus carpio var. communis. Fishes weighing 10 ± 2 gms were selected and mortality data was statistically evaluated by Finney's Probit Method. The 96-hour LC50 value for Cyprinus carpio was found as 1.1 ppm in static bioassay system. Mean values of physicochemical parameters of aquarium waters determined during bioassay depicted slight variation indicating that the mortality in aquarium fishes occurred due to pesticide exposure and not suffocation. Lab. temperature ranged from 12 to 13°C; water temperature ranged from 11 to 12°C; dissolved oxygen ranged from 3.90 to 4.56 mg/L; pH ranged from 6.90 to 7.05; total dissolved solids ranged from 2.66 to 3.0 × 103 mg/L, while CO2 remained at a constant value of 2.0 mg/L. The fishes elicited various behavioural responses such as uncoordinated movements, convulsions, excessive mucus secretion, and imbalanced swimming which ended in a collapse to the bottom of the aquarium. Prior to death, the clinical signs like scale erosion, pale body colour, and hemorrhagic patches over the body were noticed which became more vivid up to the termination of experiments. Results of the study indicate potential toxicity of dimethoate in fingerlings of common carp for which the natural waterbodies must be continuously monitored to reduce its impact across food chains. PMID:27563689

  2. Bioaccumulation of Heavy Metals and Microelements in Silver Bream (Brama brama L.), Northern Pike (Esox lucius L.), Sterlet (Acipenser ruthenus L.), and Common Carp (Cyprinus carpio L.) From Tisza River, Serbia.

    Science.gov (United States)

    Štrbac, Snežana; Kašanin-Grubin, Milica; Jovančićević, Branimir; Simonović, Predrag

    2015-01-01

    The aim of this study was to determine the concentrations of Al, As, B, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Sr, and Zn in liver, gills, gonads, and brain of four ecologically different fish species in Serbia: piscivorous northern pike, benthivorous sterlet and silver bream, and omnivorous common carp. Fish were caught at four sites along the stretch of the River Tisza in the Pannonian part of Serbia during October 2010. Results revealed that heavy metals and microelements with the highest values in fish samples were Fe, Al, and Zn. The highest concentration of heavy metals and microelements was recorded in omnivorous common carp, and organs that most intensively accumulated the greatest number of metals were liver and gills, whereas the locality did not exert a marked impact on level of bioaccumulation. PMID:26039743

  3. Radio Telemetry Study of Common Carp in Clear Lake, Iowa, To Guide Future Management

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The common carp (Cyprinus carpio) is a highly competitive freshwater fish capable of rapidly establishing populations in new, unexploited and disturbed habitats...

  4. Radio Telemetry Study of Common Carp in Clear Lake, Iowa, To Guide Future Management

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The common carp Cyprinus carpio is a highly competitive freshwater fish capable of rapidly establishing populations in new, unexploited and disturbed habitats Panek...

  5. Malheur - Telemetry Targeted Carp Movement and Removal

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Invasive common carp Cyprinus carpio were introduced into the Harney Basin in the 1920’s and were recognized as a problem in Malheur Lake in 1952. The common carp...

  6. Molecular Cloning and Characterization Analysis of NLR-C Gene in Cyprinus Carpio L.%鲤鱼NLR-C基因分子克隆及其特性分析

    Institute of Scientific and Technical Information of China (English)

    张珍; 杨桂文; 黄建勋; 彭少卿; 安利国

    2013-01-01

    We identified a NLR-C gene from carp (Cyprinus Carpio L. ) with RT-PCR and RACE, and analyzed the expression of this gene by qRT-PCR. In healthy carp,the transcript was detected with highest expression in brain and PBL,and higher in head kidney , gill, hindgut and oral epithelial. Analysis of expression of the NLR-C gene in carps after infection with Vibrio anguillarum reveales a significant up-regulation in liver,spleen,head kidney and hindgut tissues, suggesting that it is involved in the immune response of carp against the bacterial pathogen.%利用RT-PCR和RACE技术克隆得到鲤鱼(Cyprinus carpioL.)NLR-C基因cDNA全长.经qRT-PCR检测发现,NLR-C基因在鲤鱼血液和脑中表达最高,在头肾、鳃、后肠和口腔上皮中有较高表达.采用鳗弧菌(V.anguillarum)刺激后,NLR-C mRNA在肝、脾、头肾和后肠中的表达有不同程度升高.结果显示,NLR-C基因参与鲤鱼天然免疫应答过程,具有抵御病原微生物侵袭的作用.

  7. Effects of Ala-Gln feeding strategies on growth, metabolism, and crowding stress resistance of juvenile Cyprinus carpio var. Jian.

    Science.gov (United States)

    Chen, Xiu-Mei; Guo, Gui-Liang; Sun, Li; Yang, Qiu-Shi; Wang, Gui-Qin; Qin, Gui-Xin; Zhang, Dong-Ming

    2016-04-01

    The present study was conducted to evaluate the effects of different L-alanyl-l-glutamine (Ala-Gln) feeding strategies on the growth performance, metabolism and crowding stress resistance related parameters in juvenile Jian carp (Cyprinus carpio var. Jian) under crowded condition (80 g/L). Juvenile Jian carp (initial weight 26.1 ± 0.6 g) were distributed into five groups which fed with graded concentrations (0% or 1.0%) of Ala-Gln for eight weeks. Control group (I, 0/0) fed with control diet (0% Ala-Gln) throughout the feeding trial. The other four groups employed different control and experimental diet feeding strategies ranging from two weeks control diet fed and two weeks experimental diet (1% Ala-Gln) fed (II, 0/2) to eight weeks experimental diet fed (V, 4/4). Results revealed that Mean weight gain (MEG) under all different feeding strategies of Ala-Gln were significantly higher than that of the control group (p blood total protein of groups II, III and V were significantly higher than that in groups I and IV (p glucose, cortisol and catecholamine in fish. The mRNA expression of GR1a, GR1b and GR2 were also significantly changed in Ala-Gln supplementation groups compared with control group (p control group (p optimal feeding strategy was alternatively fed with control diet and then experimental diet at an interval of two weeks for juvenile Jian carp under crowded condition. PMID:26945938

  8. Effect of 4-week feeding of deoxynivalenol- or T-2-toxin-contaminated diet on lipid peroxidation and glutathione redox system in the hepatopancreas of common carp (Cyprinus carpio L.).

    Science.gov (United States)

    Pelyhe, Csilla; Kövesi, Benjámin; Zándoki, Erika; Kovács, Balázs; Szabó-Fodor, Judit; Mézes, Miklós; Balogh, Krisztián

    2016-05-01

    The purpose of study was to investigate the effects of T-2 toxin (4.11 mg T-2 toxin and 0.45 mg HT-2 toxin kg(-1) feed) and deoxynivalenol (5.96 and 0.33 mg 15-acetyl deoxynivalenol (DON) kg(-1) feed) in 1-year-old common carp juveniles in a 4-week feeding trial. The exposure of mycotoxins resulted in increased mortality in both groups consuming mycotoxin-contaminated diet. Parameters of lipid peroxidation were not affected during the trial, and antioxidant defence also did not show response to oxidative stress; however, glutatione peroxidase activity slightly, but significantly, decreased in the T-2 toxin group. Glutathione S-transferase activity showed moderate decrease as effect of T-2 toxin, which suggests its effect on xenobiotic transformation. Reduced glutathione concentration showed moderate changes as effect of DON exposure, but T-2 toxin has no effect. Expression of phospholipid hydroperoxide glutathione peroxidase (GPx4) genes showed different response to mycotoxin exposure. T-2 toxin caused dual response in the expression of gpx4a (early and late downregulation and mid-term upregulation), but continuous upregulation was found as effect of deoxynivalenol. Expression of the other gene, gpx4b, was upregulated by both trichothecenes during the whole period. The results suggested that trichothecenes have some effect on free radical formation and antioxidant defence, but the changes depend on the duration of exposure and the dose applied, and in case of glutathione peroxidase, there was no correlation between expression of genes and enzyme activity. PMID:26920403

  9. Purification of Type V Collagen from Common Carp (Cyprinus carpio) Muscle and Preparation of Polyclonal Antibody%鲤鱼肌肉Ⅴ型胶原蛋白的分离纯化及其抗体制备

    Institute of Scientific and Technical Information of China (English)

    徐超; 鲍俊旺; 蔡秋凤; 张凌晶; 刘光明; 曹敏杰

    2015-01-01

    The type V collagen from common carp muscle was extracted by pepsin digestion and salt pre⁃cipitation . SDS⁃PAGE analysis revealed that the protein contains at least two differentαchains (α1 andα2 ) . The molecular mass ofα1⁃chain was about 135 ku, while that ofα2⁃chain was around 120 ku. The character⁃istic of type V collagen is typical with triple helical structure and its denaturation temperature is 30�5℃ as as⁃sessed by circular dichroism. Purified type V collagen was used as an antigen to immunize a New Zealand rab⁃bit subcutaneously to prepare a specific polyclonal antibody. The specificity of the anti⁃collagen ( V) poly⁃clonal antibody was confirmed by Western blotting and it specifically reacted with type V collagen from fish.%通过酸溶、胃蛋白酶酶解和氯化钠盐析等方法,从鲤鱼肌肉中纯化得到了 V型胶原蛋白. SDS-PAGE电泳图谱分析发现,其至少由2条α链(α1和α2)组成,其中α1分子质量约为135 ku,α2分子质量约为120 ku.通过圆二色光谱仪分析其二级结构,发现其具有胶原蛋白三股螺旋结构的典型特征,其热变性温度为30�5℃.将V型胶原蛋白作为抗原免疫新西兰大白兔,成功制备了多克隆抗体.采用免疫印迹法对该多克隆抗体的特异性进行分析,证明其仅与鱼类V型胶原蛋白产生反应,特异性良好.

  10. 锦鲤酪氨酸酶基因序列分析及其在不同锦鲤品系不同组织中的表达%Analysis of tyrosinase gene and tissue expression in five different strains of Koi carp (Cyprinus carpio Koi)

    Institute of Scientific and Technical Information of China (English)

    王巍; 胡红霞; 孙向军; 牛翠娟

    2012-01-01

    Black is an important body color of Koi (Cyprinus carpio Koi) which is performed through melanin synthesized by melanocyte, and tyrosinase is an important rate-limiting enzyme in melanin synthesis. It's unclear of genetic mechanism of Koi body color. The present work obtained Koi tyrosinase (Tyr) gene sequence and investigated the expression of Tyr gene in different strains and tissues using semi-quantitative PCR, trying to reveal the relationship between the pigment control genes and the body color. The results showed 1 779 bp of Tyr gene, including a 1 608 bp open reading frame encoding 535 amino acids. Sequence analysis of cDNA and protein and phylogenetic analysis indicated that conservation of Tyr is higher in teleost than between teleost and other vertebrates. Semi-quantitative analysis showed the highest Tyr expression in the eye and black skin, intermediate in brain, red and yellow skin, and the lowest in liver and heart. There was no significant difference of Tyr expression between the same tissues of eyes, liver, heart, brain from different Koi carps, but differentiated in the skins with the highest in black skin, followed by red and yellow skin and small amount in white skin. The different expression was related to tissue function. The role of tyrosinase in non-melanoma cells was unclear. There possibly exist multiple forms of tyrosinase, some of which is inhibited, or the xanthophores and erythrophores could be translated into functional melanocytes.%为了解色素控制基因与体色分布的关系,实验用分子克隆技术得到了锦鲤酪氨酸酶(tyrosinase,Tyr) cDNA基因序列,并利用半定量PCR的方法分析了酪氨酸酶基因在锦鲤不同品系和组织中的表达差异.实验得到了长约1 779 bp锦鲤酪氨酸酶cDNA基因,其包括长1 608 bp开放阅读框,编码535个氨基酸.cDNA、蛋白水平序列分析和系统发育分析都表明酪氨酸酶在鱼类间的保守性要高于鱼类与其他脊椎动物间的保守性.

  11. Roles of phagocytosis activating protein (PAP) in Aeromonas hydrophila infected Cyprinus carpio.

    Science.gov (United States)

    Wonglapsuwan, Monwadee; Kongmee, Pataraporn; Suanyuk, Naraid; Chotigeat, Wilaiwan

    2016-06-01

    Cyprinus carpio (koi) is one of the most popular ornamental fish. A major problem for C. carpio farming is bacterial infections especially by Aeromonas hydrophila. Previously studies had shown that the Phagocytosis Activating Protein (PAP) gene was involved in the innate immune response of animals. Therefore, we attempted to identify a role for the PAP gene in the immunology of C. carpio. The expression of the PAP was found in C. carpio whole blood and increased when the fish were stimulated by inactivated A. hydrophila. In addition, PAP-phMGFP DNA was injected as an immunostimulant. The survival rate and the phagocytic index were significantly increased in the A. hydrophila infected fish that received the PAP-phMGFP DNA immunostimulant. A chitosan-PAP-phMGFP nanoparticle was then developed and feeded into fish which infected with A. hydrophila. These fish had a significantly lower mortality rate than the control. Therefore, this research confirmed a key role for PAP in protection fish from bacterial infection and the chitosan-PAP-phMGFP nanoparticle could be a good prototype for fish immunostimulant in the future. PMID:26748248

  12. Innate immune receptors in carp: recognition of protozoan parasites

    NARCIS (Netherlands)

    Ribeiro, C.M.S.

    2010-01-01

    This PhD thesis reports on pattern recognition receptors involved in the immune responses of common carp (Cyprinus carpio) to two protozoan parasites Trypanoplasma borreli and Trypanosoma carassii. The immune responses of carp are fundamentally different when comparing these two extracellular blood

  13. Sacharidové komponenty adenohypofysárních hormonů kapra obecného (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Ryšlavá, H.; Plíhal, O.; Selicharová, Irena; Bartová, J.

    Vodňany : VUHR, 2004, s. 201-203. [Česká ichtyologická konference /7./. Vodňany (CZ), 06.05.2004-07.05.2004] Institutional research plan: CEZ:AV0Z4055905 Keywords : saccharide component * Cyprinus carpio Subject RIV: CE - Biochemistry

  14. Carotenoid metabolism in fancy red carp, Cyprinus carpio, 2

    International Nuclear Information System (INIS)

    The absorbed free form zeaxanthin, partially stored in the intestine and hepatopancreas, is transfered to the integument directly or through the hepatopancreas. In the integument, zeaxanthin is esterified and converted to astaxanthin via adonixanthin to maintain the equilibrium among zeaxanthin ester, adonixanthin ester and astaxanthin ester. (auth.)

  15. Ontogeny of the common carp (Cyprinus carpio L.) immune system

    NARCIS (Netherlands)

    Huttenhuis, B.T.

    2005-01-01

    The production of farmed fish has increased considerably over the past years, and is now providing a significant contribution to the food market. Mortality however, can be high especially in young fish. The administration of immuno-stimulants via the food can be a sustainable approach especially in

  16. Effects of Yeast Nucleotide on Growth Performance, Body Composition and Immune Indices of Common Carp (Cyprinus carpio)%酵母核苷酸对鲤生长性能、体组成及血清免疫指标的影响

    Institute of Scientific and Technical Information of China (English)

    向枭; 周兴华; 陈建; 郑宗林

    2011-01-01

    本试验旨在研究酵母核苷酸对鲤生长性能、体组成及血清免疫指标的影响.以600尾健康的鲤[平均体重(1.09±0.07)g;平均体长(3.60±0.34)cm]为试验对象,随机分为6组(每组3个重复,每个重复30尾),分别投喂在基础饲料中添加0(对照组)、258、516、774、1 032、1 290 mg/kg酵母核苷酸(以有效成分计)的试验饲料,养殖50 d.结果表明:与对照组相比,各酵母核苷酸添加组的增重率(WGR)、特定生长率(SGR)、蛋白质效率(PER)(258 mg/kg组除外)均显著升高(P<0.05),饵料系数(FCR)则显著降低(P<0.05).当酵母核苷酸添加量为516 mg/kg时,鲤的增重率、特定生长率、蛋白质效率均达到最大(分别为372.03%、3.10%/d和195.74%),饵料系数达到最低(1.42),与其他各组差异显著(P<0.05).以增重率、特定生长率、蛋白质效率及饵料系数为指标,利用折线模型分析表明,鲤生长性能最佳时酵母核苷酸添加量为519.85~535.54 mg/kg.对鲤体组成分析表明,酵母核苷酸对鱼体粗灰分和水分含量影响不显著(P>0.05),当酵母核苷酸添加量在516~1 290 mg/kg时鱼体粗蛋白质和粗脂肪含量显著高于对照组(P<0.05).与对照组相比,各酵母核苷酸添加组的血清溶茵酶、超氧化歧化酶、过氧化氢酶活性显著升高(P<0.05),并随酵母核苷酸水平的增加先增加后降低.酵母核苷酸添加量为774~1 290 mg/kg时,血清溶茵酶、超氧化歧化酶活性较高;在酵母核苷酸添加量为516~1 032 mg/kg时,血清过氧化氢酶活性较高.综合考虑生长性能和免疫能力,鲤饲料中酵母核苷酸的适宜添加量为519.85~774.00 mg/kg.%The experiment was conducted to study the effects of yeast nucleotide on growth performance, body composition and serum immune indices of common carp ( Cyprinus carpio) . Six hundred healthy common carp [ average body weight of (1. 09 ±0. 07) g and average body length of (3. 60 ±0. 34) cm] were

  17. Genetic Status of the Original Population of Barbless Carp Cyprinus pellegrini%大头鲤原种种群的遗传现状

    Institute of Scientific and Technical Information of China (English)

    唐卫星; 陈毅峰

    2012-01-01

    大头鲤(Cyprinus pellegrini)是一种云南高原湖泊特有的国家Ⅱ级重点保护鱼类.土著种大头鲤与外来种鲤(C.carpio)的渐渗杂交在星云湖野生种群中已经广泛发生.本研究结合形态和微卫星及线粒体DNA分析检测了螺蛳铺大头鲤原种种群的遗传现状.结果显示,螺蛳铺大头鲤样本所有个体在形态上位于大头鲤与鲤的参照样本之间;在微卫星因子对应分析中也显示,其位于大头鲤与鲤的参照样本之间,但与大头鲤参照样本较为相似;在线粒体DNA分析中显示,都具有鲤的单倍型.中间性形态特征以及核基因组成与线粒体基因组成的不一致现象,表明螺蛳铺大头鲤样本均为杂种个体.因此,螺蛳铺大头鲤原种种群可能是一个杂种种群,有必要重新建立大头鲤人工繁殖计划.%Barbless Carp (Cyprinus pellegrini) , which is endemic to lakes of Yunnan plateau, is categorized into the second grade in the list of China key protected animal species. Introgressive hybridization between native Barbless Carp and exotic Common Carp (C. carpio) has occurred extensively in Xingyun Lake. This study assessed the genetic status of the Luosipu original population of Barbless Carp based on morphological, microsatellite and mitochondrial DNA analyses. Morphological analyses showed that all individuals of the Luosipu Barbless Carp sample were strictly intermediate between reference samples of Barbless and Common Carp. Factorial correspondence analysis of microsatellite data indicated that all individuals of the Luosipu Barbless Carp sample fell in between reference samples of Barbless and Common Carp, but were very near to the former. Mitochondrial DNA analysis revealed that all individuals of the Luosipu Barbless Carp sample had the mitochondrial DNA haplotypes of Common Carp. There was obvious inconsistency between nuclear and mitochondrial genetic structures in Luosipu Barbless Carp sample. These results suggest that

  18. Cloning and study of adult-tissue-specific expression of Sox9 in Cyprinus carpio

    Indian Academy of Sciences (India)

    Du Qi-Yan; Wang Feng-Yu; Hua Hui-Ying; Chang Zhong-Jie

    2007-08-01

    The Sox9 gene is one of the important transcription factors in the development of many tissues and organs, particularly in sex determination and chondrogenesis. We amplified the genomic DNA of Cyprinus carpio using degenerate primers, and found that there were two versions of Sox9 in this species: Sox9a and Sox9b, that differ in having an intron of different length (704 bp and 616 bp, respectively) in the conserved HMG box region that codes for identical amino acid sequences. We used a two-phase rapid amplification of cDNA ends (RACE) for the isolation of full-length cDNA of Sox9b. Sequence analyses revealed a 2447-bp cDNA containing 233-bp 5′ untranslated region, a 927-bp 3′ untranslated region, including poly(A), and a 1287 bp open reading frame (ORF) encoding a protein of 428 amino acids. The HMG box of 79 amino acid motif was confirmed from positions 96–174. Sequence alignment showed that the identity of amino acids of Sox9 among ten animal species, including C. carpio, is 75%, indicating that the Sox9 gene is evolutionarily quite conserved. The expression level of Sox9b gene varied among several organs of adult C. carpio, with the level of expression being highest in the brain and testis.

  19. The Possibility of Using Irradiated Khishni (Liza abu) Fish Meal Instead of the Imported Protein Sources in The Diet of Common Carp (Cprinus carpio L.)

    International Nuclear Information System (INIS)

    Two experiments were done,the first for ten week,in which fish meal (FM) were used instead of animal protein (AP). A total of (12) group of young common carps Cyprinus carpio L.(25.98±0.27 gm.) were fed on four experimental diets. The first three diets were with 4%, 8% and 12% of (FM) (total replacement),specific growth rate, final fish weight and protein efficiency ratio. Whereas, in the second experiment, a total of (15) groups of C.carpio (36,44±0.23 gm)were fed on five experimental diets in which (FM) were used by 12% and 15% instead of (AP) as well as by 10% and 15% instead of Soyabean meal . The fifth diets was with 0% (F M). Statistical analysis (CRD and Dun cans test ) showed no significant differences (P>0.05) between the experimental diet according to food conversion ratio. According to these results dried irradiated Khishni can be used as a (FM). and to be a good replacer for all (AP) and 50% of Soyabean (c.p. 44%) in the diet of common carp.) (author)

  20. Cloning and Sequence Analysis of Interleukin 10 (IL-10) Full-length cDNA from Cyprinus carpio L.

    Institute of Scientific and Technical Information of China (English)

    Xiangru FENG; Yilong CHEN; Xiao ZHAO; Wendong WANG; Junhui ZHANG; Zhenguo YANG SUN; Shengmei JIA; Qiang LU

    2012-01-01

    Abstract [Objective] This study aimed to obtain IL-IO (interleukin 10) full-length cD- NA of common carpCyprinus carpio L.) and conduct the sequence analysis. []~lethod] The differentially expressed cDNA fragment was obtained by DD-RTPCR (differential display RT-PCR). The cDNA library of peripheral blood leukocytes which were separated from common carp and stimulated by mitogen was screened with a probe labeled with DIG (digoxigenin). The IL-IO full-length cDNA was cloned from 0.8x104 pfu of recombinant phages, and the sequence analysis and homology com- parison were carried out. [Result] Sequence analysis indicated that the IL-IO full- length cDNA of common carp was 1 117 bp long, containing a.55 bp 5'-UTR, a 522 bp 3"-UTR, and a 540 bp open reading frame(ORF) encoding 179 amino acids. In addition, there were three mRNA instability motifs (ATTTA) in the 3"-untranslated region. The deduced protein sequence shared typical sequence features of the IL-IO family. Homology comparison indicated that the obtained sequence shared 89.1% homology with the carp IL-IO gene from GenBank. [Conclusion] This study laid foun- dation for further study of the expression manner, functional characteristic and regu- lation mechanism of IL-IO in vivo and the interaction mechanism in the inflammatory reaction and immune response.

  1. Genetic diversity and selective breeding of red common carps in China

    OpenAIRE

    Li, S. F.; C. H. Wang

    2001-01-01

    China has a very rich genetic diversity in common carp (Cyprinus carpio) and the red common carp plays an important role in Chinese aquaculture and genetic studies. Selective breeding, particularly crossbreeding has been applied successfully to red common carps in China, and the products of these efforts have been in commercial use since the 1970s. However, knowledge of the quantitative and molecular genetics of these carps is limited. Studies were therefore undertaken to: (1) understand the ...

  2. Accumulation and Elimination of 137Cs Radionuclide by gold fish (Cyprinus Carpio)

    International Nuclear Information System (INIS)

    A study to estimate accumulation and elimination of 137Cs radionuclide by gold fish (Cyprinus Carpio) has been carried out. The experiment used aquarium as a cultivating media. Gold fish was placed into aquarium filled with 70 liter of water and 137Cs concentration of 10 Bq/ml. From the observing time of 40 days it was found that activity concentration in fish became saturated in 30th day. Saturation after the 30th day was confined by additional observation using 137Cs concentration of 5 and 15 Bq/ml. Saturated concentration fish was then transfered to inactive aquarium to determine is elimination rate. Transfer factor, i.e. the ratio of 137Cs concentration in fish to that in water, was found to be (12.99+0.28) ml/g, whereas the elimination rate of 137Cs was found to be 0.046 day, which correspond to a biological half life of 15 days

  3. Uptake of 137Cs in cultured fresh water fish (Cyprinus carpio): physiological and histological effects

    International Nuclear Information System (INIS)

    An experiment was conducted in fresh-water fish (Cyprinus carpio) cultured, in small water tanks, artificially contaminated with radioactive 137Cs (3000 Bq/1) to determine the uptake of 137Cs and its physiological and histological effects in different fish organs. It was found that 137Cs was located in muscular tissues, gills, head muscles, liver and kidneys. Moderate amounts were found in spleen, eyes, gonads, intestine and urinary bladder. It seems that sorption was of much less importance than ingestion in the uptake of 137Cs. The histological examination in musculature tissue, revealed an acute hyperemia with focal haemorrages which may be due to allergic effects of 137Cs. Hyperemia and focal fatty degeneration of hepatic cells was also noted in the liver which may be due to toxic effects of 137Cs. Diffused hyperemia has also occurred in the brain and focal degeneration of epithelial cells of renal tubules. (Author)

  4. Toxicity profile of choline chloride-based deep eutectic solvents for fungi and Cyprinus carpio fish.

    Science.gov (United States)

    Juneidi, Ibrahim; Hayyan, Maan; Mohd Ali, Ozair

    2016-04-01

    An investigation on the toxicological assessment of 10 choline chloride (ChCl)-based deep eutectic solvents (DESs) towards four fungi strains and Cyprinus carpio fish was conducted. ChCl was combined with materials from different chemical groups such as alcohols, sugars, acids and others to form DESs. The study was carried out on the individual DES components, their aqueous mixture before DES formation and their formed DESs. The agar disc diffusion method was followed to investigate their toxicity on four fungi strains selected as a model of eukaryotic microorganisms (Phanerochaete chrysosporium, Aspergillus niger, Lentinus tigrinus and Candida cylindracea). Among these DESs, ChCl:ZnCl2 exhibited the highest inhibition zone diameter towards the tested fungi growth in vitro, followed by the acidic group (malonic acid and p-toluenesulfonic acid). Another study was conducted to test the acute toxicity and determine the lethal concentration at 50 % (LC50) of the same DESs on C. carpio fish. The inhibition range and LC50 of DESs were found to be different from their individual components. DESs were found to be less toxic than their mixture or individual components. The LC50 of ChCl:MADES is much higher than that of ChCl:MAMix. Moreover, the DESs acidic group showed a lower inhibition zone on fungi growth. Thus, DESs should be considered as new components with different physicochemical properties and toxicological profiles, and not merely compositions of compounds. PMID:26743645

  5. Transcriptomic analysis of koi (Cyprinus carpio) spleen tissue upon cyprinid herpesvirus 3 (CyHV3) infection using next generation sequencing.

    Science.gov (United States)

    Lee, Xuezhu; Yi, Yang; Weng, Shaoping; Zeng, Jie; Zhang, Hetong; He, Jianguo; Dong, Chuanfu

    2016-02-01

    Cyprinid Herpesvirus 3 (CyHV-3) can infect and specifically cause a huge economic loss in both common carp (Cyprinus carpio) and its ornamental koi variety. The molecular mechanisms underlying CyHV-3 infection are not well understood. In this study, koi spleen tissues of both mock and CyHV-3 infection groups were collected, and high-throughput sequencing technology was used to analyze the differentially expressed genes (DEGs) at the transcriptome level. A total of 105,356,188 clean reads from two libraries were obtained. After the de novo assembly of the transcripts, 129,314 unigenes were generated. Of these unigenes, 70,655 unigenes were matched to the known proteins in the database, while 2190 unigenes were predicted by ESTScan software. Comparing the infection group to the mock group, a total of 23,029 significantly differentially expressed unigenes were identified, including 10,493 up-regulated DEGs and 12,536 down-regulated DEGs. GO (Gene Ontology) annotation and functional enrichment analysis indicated that all of the DEGs were annotated into GO terms in three main GO categories: biological process, cellular component and molecular function. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis of the DEGs showed that a total of 12,002 DEG unigenes were annotated into 256 pathways classified into 6 main categories. Additionally, 20 differentially expressed genes were validated by quantitative real-time PCR. As the first report of a transcriptome analysis of koi carp with CyHV-3 infection, the data presented here provide knowledge of the innate immune response against CyHV-3 in koi carp and useful data for further research of the molecular mechanism of CyHV-3 infection. PMID:26690666

  6. In vitro antioxidant and hepatoprotective potential of Azolla microphylla phytochemically synthesized gold nanoparticles on acetaminophen - induced hepatocyte damage in Cyprinus carpio L.

    Science.gov (United States)

    Kunjiappan, Selvaraj; Bhattacharjee, Chiranjib; Chowdhury, Ranjana

    2015-06-01

    The present study aims to evaluate the hepatoprotective and antioxidant effects of gold nanoparticles (GNaP) biosynthesized through the mediation of Azolla microphylla and A. microphylla extract on acetaminophen-induced hepatocyte damage in common carp fish (Cyprinus carpio L.). The gold nanoparticles (100, 150, 200 μg/ml) and A. microphylla extract powder (100, 200, 400 μg/ml) were added to the primary hepatocytes in different conditions: treatment I (before 12 mM acetaminophen), treatment II (after 12 mM acetaminophen), and treatment III (both before and after 12 mM acetaminophen), and incubated. Among these, control group treated with 12 mM acetaminophen produced significantly elevated levels (50-80%) of lactate dehydrogenase (LDH), catalase (CAT), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and malondialdehyde (MDA), and significantly decreased the levels (60-75%) of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Treatment with methanol extract of A. microphylla phytochemically biosynthesized gold nanoparticles (100, 150, 200 μg/ml) and A. microphylla methanol extract powder (100, 200, 400 μg/ml) significantly improved the viability of cells in a culture medium. It also significantly reduced the levels of LDH, CAT, GOT, GPT, and MDA, and significantly increased the levels of SOD and GSH-Px. In conclusion, gold nanoparticles biosynthesized through A. microphylla demonstrated effective hepatoprotective and antioxidant effects than methanol extract of A. microphylla. PMID:25862331

  7. Monitoring the effects of water pollution on Cyprinus carpio in Karakaya Dam Lake, Turkey.

    Science.gov (United States)

    Ozmen, Murat; Güngördü, Abbas; Kucukbay, F Zehra; Güler, R Elif

    2006-03-01

    Karakaya Dam Lake (KDL) is one of the most important water sources, both for irrigation and fishery, located in eastern part of Turkey. This study is concerned with the pollution of the lake contributed by urban, industrial and agricultural activities. The parameters selected for this aim were the enzymes commonly used as biomarkers of environmental pollution. The activity of glutathione S-transferase (GST), carboxylesterase (CE), lactate dehydrogenase (LDH), acid phosphatase (ACP) and aspartate amino transferase (AST) has been determined in liver tissue samples of Cyprinus carpio, a representative species of KDL. Furthermore, brain acetylcholinesterase (AChE) activity which is mainly affected by pesticides such as organophosphates, has been assayed. Chemical analysis results showed that KDL was polluted by various heavy metals as it was apparent from water, sediment and gill tissue. The activity of brain AChE was significantly lower in all localities than Tecimli area (St-5) where there is no agricultural and industrial activities in the immediate periphery. Thus, this change of AChE activity may relate to agricultural pollution in KDL. On the other hand, no significant differences were found for selected enzyme biomarkers, but condition factor (CF) or hepatosomatic index were significantly different from the St-5 samples, a result that may be attributed to water pollution in KDL by various contaminants. PMID:16374666

  8. Characterization of Branchial Na,K-ATPase from Three Freshwater Fish Species (Oreochromis niloticus, Cyprinus carpio, and Oncorhynchus mykiss)

    OpenAIRE

    Atli, Gülüzar; CANLI, Mustafa

    2008-01-01

    Branchial Na,K-ATPase activity was characterized in 3 freshwater fish species (Oncorhynchus mykiss, Oreochromis niloticus, and Cyprinus carpio) with different ecological needs. Na+, K+, and Cl- concentrations in the gills were also measured. The maximal Na,K-ATPase activity was observed at 100 mM Na+, 20-40 mM K+, 3-4 mM Mg2+, and 1 mM ouabain. The maximal velocity (Vmax) of Na,K-ATPase isolated from O. mykiss (1.07 µmol Pi/mg prot/h) was lower than that isolated from O. niloticus (7.25 µmol ...

  9. Blood Picture and Enzymatic Activities in Common Crap Cyprinus carpio Influenced by Sodium Chloride (NaCl)

    OpenAIRE

    Al-Taee, Shahba Kale Al-Taee; Al-Hamdani, Alaa Hussain

    2014-01-01

    The aim of this study was to assess the effect of Sodium Chloride NaCl on blood picture as hemoglobin (Hb), packed cell volume (PCV) and lymphocyte count also the activity of both enzymes Alanine Amino Transferase (ALT) and Creatine Phospho Kinase (CPK) in Cyprinus carpio. NaCl was used at different concentration (0.5; 1.0 and 1.5 mg\\L) for 7 day. The concentration 1.5 mg\\L was toxic to the fish and caused death after 48 hour of exposure, while in the fish that were exposure separately to bot...

  10. Effect of Astralagus radix on proliferation and nitric oxide production of head kidney macrophages in Cyprinus carpio: an in vitro study

    NARCIS (Netherlands)

    Guo-Jun, Y.; Wiegertjes, G.F.; Yue-Ming, L.; Schrama, J.W.; Verreth, J.A.J.; Pao, X.; Hong-qi, Z.

    2004-01-01

    The in vitro effect of Astragalus radix water extract onthe non-specific immune responses of macrophages isolated from the head kidney of Cyprinus carpio was evaluated. Astragalus radix extract stimulated the proliferation of the head kidney macrophages, and it alone had no effect on nitrogen burst

  11. Fullerene inhibits benzo(a)pyrene Efflux from Cyprinus carpio hepatocytes by affecting cell membrane fluidity and P-glycoprotein expression.

    Science.gov (United States)

    Chen, Qiqing; Hu, Xialin; Wang, Rui; Yuan, Jin; Yin, Daqiang

    2016-05-01

    P-Glycoprotein (P-gp) can protect cells by pumping out toxic compounds, and has been found widely expressed in fish tissues. Here, we illustrate the P-gp efflux ability for benzo(a)pyrene (BaP) in the hepatocytes of common carp (Cyprinus carpio) after exposing to fullerene aqueous suspension (nC60). The results revealed that nC60 increased the membrane fluidity by decreasing the ratio of saturated to unsaturated fatty acids, and increased the cholesterol contents. These findings, combined with 10-38% and 70-75% down-regulation of P-gp mRNA and protein respectively, suggested that nC60 caused inhibition on P-gp efflux transport system. Therefore, we further investigated the cellular efflux ability for BaP. Results showed unequivocally that nC60 is a potent P-gp inhibitor. The retaining BaP amounts after efflux were elevated by 1.7-2.8 fold during the 10 day exposure. Meanwhile, 5mg/L humic acid (one of the important fractions of natural organic matter, which is ubiquitous in aquatic environment) alleviated the nC60 damage to hepatocytes in terms of oxidative damage, cholesterol increment, and P-gp content reduction; and finally attenuated the suppressed P-gp efflux ability. Collectively, this study provides the first evidence of nC60 toxicity to P-gp functionality in fish and illustrates the possible mechanism of the suppressed P-gp efflux ability for BaP. PMID:26918948

  12. The invasive fish tapeworm Atractolytocestus huronensis (Cestoda), a parasite of carp, colonises Africa

    Czech Academy of Sciences Publication Activity Database

    Scholz, Tomáš; Tavakol, S.; Halajlan, A.; Luus-Powell, W.J.

    2015-01-01

    Roč. 114, č. 9 (2015), s. 3521-3524. ISSN 0932-0113 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Biological invasions * Helminth parasites * Fish * Common carp * Cyprinus carpio * Africa Subject RIV: EG - Zoology Impact factor: 2.098, year: 2014

  13. MONTHLY VARIATION IN SPERM MOTILITY IN COMMON CARP ASSESSED USING COMPUTER-ASSISTED SPERM ANALYSIS (CASA)

    Science.gov (United States)

    Sperm motility variables from the milt of the common carp Cyprinus carpio were assessed using a computer-assisted sperm analysis (CASA) system across several months (March-August 1992) known to encompass the natural spawning period. Two-year-old pond-raised males obtained each mo...

  14. Physico-Chemical, Functional and Antioxidant Properties of Roe Protein Concentrates from Cyprinus carpio and Epinephelus tauvina

    Directory of Open Access Journals (Sweden)

    Galla Narsing Rao

    2014-09-01

    Full Text Available Roe protein concentrates prepared from Cyprinus carpio (CRPC and Epinephelus tauvina (ERPC were investigated for physico-chemical characteristics, functional properties and antioxidant activity. Cyprinus and Epinephelus roes yielded 19.5% and 21.5% of protein concentrates possessing 70.71% and 85.9% protein, respectively. Moisture sorption isotherms of roe protein concentrates indicated the non-hygroscopic nature of CRPC with initial moisture content (IMC of 8%, which equilibrated at 63% RH and hygroscopic nature of ERPC with IMC of 4.9%, which equilibrated at 42% RH. Water absorption capacity, oil absorption capacity, foam capacity and lower foam stability and emulsifying capacity were found in ERPC than in CRPC respectively. Maximum solubility of protein was 17.79% and 16.89% at pH 12, observed in CRPC and ERPC respectively. Higher buffer capacity was observed in both roe protein concentrates in alkali medium. Antioxidant activity determined by the DPPH radical scavenging activity was higher, and ferric reducing power was observed to be lower in ERPC.

  15. VARIASI KOMBINASI TEPUNG LABU KUNING (Cucurbita moschata D.) DAN TEPUNG AZOLLA (Azolla pinnata R.br.) PADA KECERAHAN WARNA IKAN KOI (Cyprinus carpio L.)

    OpenAIRE

    Utami, Diah Ayu Tri

    2014-01-01

    Warna memegang peranan penting dalam menentukan kualitas ikan koi (Cyprinus carpio L.), bahkan dipandang sebagai komponen penting dalam proses seleksi ikan hias. Permasalahan yang sering timbul adalah memudarnya warna ikan bila dipelihara dalam jangka waktu yang lama. Penelitian ini menambahkan tepung labu kuning (Cucurbita moschata D.) dan tepung Azolla (Azolla pinnata R.Br.) ke dalam pakan buatan. Dengan penambahan tepung labu kuning dan tepung Azolla ini memungkinkan ikan memperoleh lebih ...

  16. Effect of mola (Amblypharyngodon mola Ham.) on the growth and production of carps in polyculture

    OpenAIRE

    Kohinoor, A.H.M.; Islam, M.L; Wahab, M. A.; Thilsted, S.H.

    1998-01-01

    The effect of introduction of mola (Amblypharyngodon mala) in polyculture with rohu (Labeo rohita), catla (Catla catla) and mirror carp (Cyprinus carpio var. specularis) was studied in semi-intensive culture system in the pond complex of the Fisheries Faculty, Bangladesh Agricultural University, Mymensingh. Mala, a small indigenous fish was found to exert a negative impact on growth and production of carps. After four months' rearing, significant difference (P

  17. Life Cycle Assessment for environmentally sustainable aquaculture management : a case study of combined aquaculture systems for carp and tilapia

    OpenAIRE

    Mungkung, R.; Aubin, J.; Prihadi, T. H.; Slernbrouck, J.; van der Werf, H. M. G.; Legendre, Marc

    2013-01-01

    Life Cycle Assessment (LCA) was applied to evaluate the potential environmental impacts associated with two-net cage aquaculture systems of common carp (Cyprinus carpio carpio) and tilapia (Oreochromis niloticus) in the Cirata reservoir, Indonesia. The studied system included fingerling production in hatcheries, fish rearing in cages, and transport of fry and feed as well as that of harvested fish to markets. The environmental impact indicators were calculated based on the annual production i...

  18. Trypanoplasma borreli cystein proteinase activities support a conservation of function with respect to digestion of host proteins in common carp

    NARCIS (Netherlands)

    Ruszczyk, A.; Forlenza, M.; Joerink, M.; Ribeiro, C.M.S.; Jurecka, P.M.; Wiegertjes, G.F.

    2008-01-01

    Trypanoplasma borreli is an extracellular parasite that is transmitted by a leech vector and is naturally found in the blood of cyprinid fish. High parasitemia and associated severe anemia together with splenomegaly are typical of infection of common carp, Cyprinus carpio L. Papain-like cysteine pro

  19. Benzo(a)pyrene-induced cytochrome p4501A expression of four freshwater fishes (Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus).

    Science.gov (United States)

    Lee, Jin Wuk; Yoon, Hong-Gil; Lee, Sung Kyu

    2015-05-01

    Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus are useful indicator species for CYP1A biomarker studies; however, comparative studies have not been performed. To compare susceptibility, dose- and time-dependent CYP1A induction at the mRNA and protein levels in response to benzo(a)pyrene (BaP) exposure was analyzed. At the mRNA level, a statistically significant difference was found among the four species; however, such was not observed at the protein level. C. carpio showed the highest CYP1A induction level and the steepest slope in the dose-response curve. To assess susceptibility, the difference in CYP1A mRNA induction among species must be considered, and C. carpio was the most sensitive species of the four evaluated in terms of CYP1A expression. PMID:25863331

  20. Study of the portals of entry of Cyprinid herpesvirus 3 in Cyprinus carpio

    OpenAIRE

    Fournier, Guillaume

    2012-01-01

    The common carp is one of the most important freshwater species in aquaculture and its colourful subspecies koi is grown for personal pleasure and exhibitions. Both subspecies are economically important. In the 1990s, a highly contagious and lethal pathogen called koi herpesvirus (KHV) or cyprinid herpesvirus 3 (CyHV-3) began to cause severe financial losses in these two carp industries worldwide. Because of its economic importance and its numerous original biological properties, CyHV 3 becam...

  1. Biomarkers of Cytotoxic, Genotoxic and Apoptotic Effects in Cyprinus carpio Exposed to Complex Mixture of Contaminants from Hospital Effluents.

    Science.gov (United States)

    Olvera-Néstor, Corina G; Morales-Avila, Enrique; Gómez-Olivan, Leobardo M; Galár-Martínez, Marcela; García-Medina, Sandra; Neri-Cruz, Nadia

    2016-03-01

    Hospital wastewater is an important source of emerging contaminants. Recent studies emphasize the importance of assessing the effects of mixtures of contaminants rather than environmental risk of their individual components, as well as the determination of intrinsic toxicity of wastewater. Mixtures of pollutants has possible interactions that have notable environmental side effects. The aim of this study is an attempt to characterize biomarkers in Cyprinus carpio related to the exposure to a complex mixture of contaminants found in hospital wastewater. Results of a particular hospital effluent show the presence of traces of heavy metals, high chlorine concentration and emerging contaminants such as non-steroidal anti-inflammatory drugs. The LC50 was of 5.49 % at 96 h. The cytotoxic, genotoxic and apoptotic biomarkers increase when fishes were exposed to wastewater (1/10 CL50) from hospital wastewater. This study emphasizes the importance of identifying and quantifying the effects of contaminants as pharmaceuticals, disinfectants and surfactants in order to design and implement an ecotoxicological plan. PMID:26754545

  2. ESTIMATION OF VOLATILE CONSTITUENTS IN THE FISH FLESH FROM WILD AND FARMED CIRRHINA MRIGALA AND CYPRINUS CARPIO

    Institute of Scientific and Technical Information of China (English)

    Shahid Mahboob; Bilal Hussain; Zahid Iqbal; Abdul Shakoor Chaudhry

    2009-01-01

    Analysis of fish meat using gas chromatography is described. Flavor is the sensation arising from the interplay of the signals of sensing smell, taste and irritating stimuli from food stuff. For human, flavor and nutrition are inseparable. In fish, trace amount of volatile organic compounds (VOCs) are the major compounds to affect consumer's preference, which are produced during storage and spoilage. In the present study, volatile compounds were extracted by Likens-Nickerson con-current distillation apparatus from wild and farmed Cirrhina mrigala and Cyprinus carpio. The quantitative and qualitative estimation of volatiles was made by gas chromatography. Wild and farmed fish of different fish sizes were compared for these compounds (appearing in the form of peaks), which were identified from their retention time by comparing with the stand-ards. Fifteen major VOCs were found in these species which included hexadecane, 3-octanol, hexanal, decane, 3-hexene-1-ol, 2-undecanone, 2-heptanone, butanal, 2-nonanone, 1-heptanal, furaldehyde, 3-methyl-1-butanal, trans-3-hexene-1-ol, octanal and decanal. These compounds varied qualitatively and quantitatively in both wild and farmed fish of different fish sizes.

  3. Electrical coupling between bipolar cells in carp retina

    OpenAIRE

    Kujiraoka, Toru; Saito, Takehiko

    1986-01-01

    Intracellular recordings were made simultaneously from pairs of neighboring bipolar cells by advancing two independent microelectrodes into retinas of carp (Cyprinus carpio). Bipolar cells were identified by their response properties and in several samples were verified by intracellular injection of Lucifer yellow. Current of either polarity injected into one member of the bipolar cell pair elicited a signconserving, sustained potential change in the other bipolar cell without any significant...

  4. The Major Portal of Entry of Koi Herpesvirus in Cyprinus carpio Is the Skin▿

    Science.gov (United States)

    Costes, B.; Raj, V. Stalin; Michel, B.; Fournier, G.; Thirion, M.; Gillet, L.; Mast, J.; Lieffrig, F.; Bremont, M.; Vanderplasschen, A.

    2009-01-01

    Koi herpesvirus (KHV), recently designated Cyprinid herpesvirus 3, is the causative agent of a lethal disease in koi and common carp. In the present study, we investigated the portal of entry of KHV in carp by using bioluminescence imaging. Taking advantage of the recent cloning of the KHV genome as a bacterial artificial chromosome (BAC), we produced a recombinant plasmid encoding a firefly luciferase (LUC) expression cassette inserted in the intergenic region between open reading frame (ORF) 136 and ORF 137. Two viral strains were then reconstituted from the modified plasmid, the FL BAC 136 LUC excised strain and the FL BAC 136 LUC TK revertant strain, including a disrupted and a wild-type thymidine kinase (TK) locus, respectively. In vitro, the two recombinant strains replicated comparably to the parental FL strain. The FL BAC 136 LUC TK revertant strain was shown in vitro to induce a bioluminescent signal allowing the detection of single positive cells as early as 24 h postinfection, while in vivo, it induced KHV infection in carp that was indistinguishable from that induced by the parental FL strain. To identify the KHV portal of entry, carp were analyzed by bioluminescence imaging at different times postinfection with the FL BAC 136 LUC TK revertant strain. These analyses demonstrated that the skin of the fish covering the fins and also the body is the major portal of entry for KHV in carp. Finally, to further demonstrate the role of the skin as the KHV portal of entry, we constructed an original system, nicknamed “U-tube,” to perform percutaneous infection restricted to the posterior part of the fish. All the data obtained in the present study demonstrate that the skin, and not the gills, is the major portal of entry for KHV in carp. PMID:19153228

  5. Activity of Superoxide Dismutase in Different Tissues of Cyprinus carpio var under Acid and Aluminum Stress%酸和铝胁迫对锦鲤不同组织SOD活性的影响

    Institute of Scientific and Technical Information of China (English)

    周小玲; 洪华嫦; 王方园; 丁林贤; 林红军; 高杨

    2012-01-01

    This paper report on an experimental study about the enzyme activity of superoxide dismutase (SOD) in carp's organs (brain, kidney and liver), which were exposed to aid and/or Al. The results showed that SOD activity of brain was remarkably inhibited the first day after the exposures to acid and/or Al; however, SOD recovered and returned to being almost normal the third day for the test group of joint exposure to acid and Al, and SOD recovered the ninth day for the test group of singly exposure to acid or Al. The effects of the exposure on SOD activity of kidney and liver of carp (Cyprinus carpio var) were also described.%该实验目的是研究酸(pH=5,Al=0 μg/L)、铝(pH=7,Al=800 μg/L)单独及联合(pH=5,Al=800 μg/L)作用下,锦鲤鱼脑、肾、肝超氧化物歧化酶(SOD)活性的反应变化情况.结果表明,与对照组(pH=7,Al=0 μg/L)相比,锦鲤鱼脑无论在酸、铝单独作用还是联合作用下,SOD活性在染毒第1天均显著降低(P<0.01),但随后酸铝联合组、单独作用组分别在3d、9d恢复正常;肾SOD则只有在酸铝联合作用下,且只有在染毒第1天产生显著抑制(P<0.01),随后也恢复正常;肝SOD则无论在何种情况下,均无抑制现象发生.

  6. [Investigations of ectoparasite fauna of some fish species (Cyprinus carpio Linnaeus, 1758; Cobitis simplicispinna Hanko, 1924) from Lake Akşehir (Konya)].

    Science.gov (United States)

    Kartal, Kürşat; Oztürk, Mehmet Oğuz

    2009-01-01

    In this study, the presence of metazoan ectoparasites on 7 Cyprinus carpio and 90 Cobitis simplicispinna from Lake Aksehir, Turkey were investigated between July 2004 and June 2005. Three parasite species were identified on the host fishes: Gyrodactylus elegans Nordmann, 1832 (42.9%, 293.6+/-482.0 parasite/fish) and Dactylogyrus extensus Mueller and Van Cleave, 1932 (85.7%, 9.8+/-6.8), were found on gills of C. carpio, and Gyrodactylus cobitis Bychowsky, 1933 (68.9%, 15.6+/-18.5) on gills and fins of C. simplicispinna. Of these species, G. cobitis is a new record for parasite fauna of Turkey. In addition, minimum-maximum and mean intensity of parasites and infection prevalence were determined using seasonal data and size distribution of the host fish. PMID:19367558

  7. Optimalisering van de groei bij de karper (Cyprinus carpio L.) : een op de visteelt gericht onderzoek

    NARCIS (Netherlands)

    Huisman, E.A.

    1974-01-01

    In course of time carp culture - in Europe already practiced since the Middle Ages - has been subjected to different patterns of intensification e.g. fertilization of ponds and supplementary feeding. Mechanisation in pond farm management has also proved to be important.The aim of these measures has

  8. Potensi Fitoplankton sebagai Sumber Daya Pakan pada Pemeliharaan Larva Ikan Mas (Cyprinus carpio di BBPBAT Sukabumi

    Directory of Open Access Journals (Sweden)

    Ana Widiana

    2016-03-01

    Full Text Available The main problem encountered in producing larvae  is the larvae  that got low due to high mortality. One attempt to overcome this problem is the use of phytoplankton as natural feed. The purpose of this study is to determine phytoplankton community structure and potential as a resource phytoplankton feed on larval rearing carp. The research was conducted in April 2012. The results showed that the phytoplankton found in the larval rearing pond goldfish are Class Bacillariophyceae (1 genera, Chlorophyceae (13 genera, and Cyanophyceae (7 genera. Phytoplankton Chlorophyceae group is a group that has the highest abundance of larval rearing carp pond. Diversity index of phytoplankton in pond carp larvae ranged from 0.65 to 1.64 (Low Diversity. Based on the preponderance index carp larvae utilize phytoplankton as natural feed by selecting the class Chlorophyceae and Cyanophyceaea in early growth until the time nursery a preponderance index value respectively 87.76% and 12.23%.

  9. Skin mucus of Cyprinus carpio inhibits cyprinid herpesvirus 3 binding to epidermal cells

    Directory of Open Access Journals (Sweden)

    Raj Victor

    2011-08-01

    Full Text Available Abstract Cyprinid herpesvirus 3 (CyHV-3 is the aetiological agent of a mortal and highly contagious disease in common and koi carp. The skin is the major portal of entry of CyHV-3 in carp after immersion in water containing the virus. In the present study, we used in vivo bioluminescence imaging to investigate the effect of skin mucus removal and skin epidermis lesion on CyHV-3 entry. Physical treatments inducing removal of the mucus up to complete erosion of the epidermis were applied on a defined area of carp skin just before inoculation by immersion in infectious water. CyHV-3 entry in carp was drastically enhanced on the area of the skin where the mucus was removed with or without associated epidermal lesion. To investigate whether skin mucus inhibits CyHV-3 binding to epidermal cells, tail fins with an intact mucus layer or without mucus were inoculated ex vivo. While electron microscopy examination revealed numerous viral particles bound on the fins inoculated after mucus removal, no particle could be detected after infection of mucus-covered fins. Finally, anti-CyHV-3 neutralising activity of mucus extract was tested in vitro. Incubation of CyHV-3 with mucus extract reduced its infectivity in a dose dependent manner. The present study demonstrates that skin mucus removal and epidermal lesions enhance CyHV-3 entry in carp. It highlights the role of fish skin mucus as an innate immune protection against viral epidermal entry.

  10. Reutilización del agua residual tratada en la Unidad Académica Profesional Amecameca de la UAEM en la producción de Cyprinus carpio specularis para consumo humano. (“Sewage water second use, treated in Amecameca at the Academic Unit UAEM in the production of Cyprinus carpio specularis human intake”

    Directory of Open Access Journals (Sweden)

    Hernández Hernández, Armando

    2006-07-01

    July 2004 and finished in November 2005 consisted on the following: carp (Cyprinus carpio specularis was raised using the water coming from the UAP Amecameca treatment plant which has a electrofloculation anaerobic-aerobic process. The water was concentrated at 10% proportions (treated water physic and chemic factors were constantly measure (DBO5, DQO, OD, NH3-, NO2-, NO3-, pH, conductivity, temperature, suspended solids, dissolved solids, turbidityand biological factors ( totally coliforms and fecal coliforms. Carps were nourished also with feeding portions of corn, and worms both produced at the UAP Amecameca. Bird food was included too. It was concluded that when we used recycled water the carps obtained are human intake as stated by the Mexican Official Rule (NOM-112-SSA!-1004. However the quantity of production obtained was not as good as the one produced in a current water which parameters are just competent. Lab analysis reported the following parasites in the fish (Lernaea cyprinacea and acuatic louse (Argulus sp, a negative gram bacillus identified as Shigella boydi. Regarding the diet under the three intakes feeding the carps, and once the basic needs were covered body weight growth from Junuary to July 2005 as much as 35 g. minimum and 45 maximum. Results show that there was a slight body weight growth in those fish fed with corn and worm flour. However when variety of analysis was performed it did not show an important variation.

  11. Quantitative structure-activity relationships for the Toxicity of Substituted Benzenes to Cyprinus carpio

    Institute of Scientific and Technical Information of China (English)

    GUANG-HUA LU; CHAO WANG; XING YUAN; PEI-ZHEN LAN

    2005-01-01

    Objective To measure the 96h-LC50 values of 32 substituted benzenes to the carp and to study the relationship between quantitative structure-activity and structural parameters of chemicals. Methods The acute toxicity values of 32 substituted benzenes to the carp were determined in a semistatic test. The energy of the lowest unoccupied molecular orbital, and the highest occupied molecular orbital, the dipole moment and the molecular weight of substituted benzenes were calculated by the quantum chemical method MOPAC6.0. Results The range of the toxicity of studied compounds was broad, and the most toxic compound was pentachlorophenol, while the least toxic compound was 4-methylaniline. By the stepwise regression analyses, a series of Quantitative structure-activity relationships (QSAR) equations were derived from all compounds and subclasses. The equation log1/LC50=0.759logP +2.222 (R2 (adj)=0.818) was found to fit well and the average predicted percentage error was 6.16%. Conclusion The toxicity of anilines and phenols to the carp could be modeled well by logP alone, whereas the toxicity of the halogenated benzenes and nitrobenznes not containing hydroxyl or amino group can be controlled by hydrophobic and electronic factors.

  12. Toxicity of furadan (carbofuran 3% g in Cyprinus carpio: Haematological, biochemical and enzymological alterations and recovery response

    Directory of Open Access Journals (Sweden)

    Mathan Ramesh

    2015-12-01

    Full Text Available Furadan, a carbamate pesticide is widely used in paddy fields and it has been detected in ground, surface and rain waters. In this study, fingerlings of Cyprinus carpio were exposed to different concentrations of furadan ranging from 25 to 50 mg L−1 for 96 h and the acute toxicity was calculated as 43.651 mg L−1. To assess the effect of furadan, fish were exposed to two concentrations of furadan (8.730 mg L−1, Treatment I and 4.365 mg L−1, Treatment II and certain haematological, biochemical and enzymological parameters were evaluated at the end of 24, 48, 72 and 96 h exposure periods with a recovery period of 96 h. A significant (p < 0.05 decrease in haemoglobin (Hb, haematocrit (Hct, red blood cells (RBC, plasma protein and glutamate oxaloacetate transaminase (GOT activity in gill, liver and kidney (except at the end of 96 h in Treatment I were noted in both the concentrations tested while white blood cells (WBC and glucose level were significantly increased after 24, 48, 72 and 96 h exposure periods when compared to untreated groups. A mixed trend in mean corpuscular volume (MCV, mean corpuscular haemoglobin (MCH and mean corpuscular haemoglobin concentration (MCHC and glutamate pyruvate transaminase (GPT activity in gill, liver and kidney was noted. During the recovery study period (96 h MCHC and plasma glucose level was recovered to some extent whereas the other parameters remain altered. The alterations of these parameters can be used to assess the toxic levels of the pesticide furadan on aquatic biota.

  13. Behavioral Responses of the Freshwater Fish, Cyprinus carpio (Linnaeus) Following Sublethal Exposure to Chlorpyrifos

    OpenAIRE

    Halappa, Ramesh; David, Muniswamy

    2009-01-01

    Common carp fingerlings were exposed to different concentrations (0.120 to 0.200 mg/L) of an organophosphate pesticide, chlorpyrifos (20% EC) for 96 h. The acute toxicity (LC50) of chlorpyrifos by static renewal (semi-static) bioassay test was found to be 0.160 mg/L. One-seventh (0.0224 mg/L) and one-fourteenth (0.0112 mg/L) of the 96 h LC50 were selected as sublethal concentrations for subacute studies. The fish were exposed to both the sublethal concentrations for 1, 7 and 14 days and allow...

  14. Molecular characterization of Sphaerospora molnari (Myxozoa), the agent of gill sphaerosporosis in common carp (Cyprinus carpio carpio)

    Czech Academy of Sciences Publication Activity Database

    Eszterbauer, E.; Sipos, D.; Forró, B.; Bartošová, Pavla; Holzer, Astrid S.

    2013-01-01

    Roč. 104, č. 1 (2013), s. 59-67. ISSN 0177-5103 R&D Projects: GA ČR GBP505/12/G112; GA ČR(CZ) GPP506/11/P724 Grant ostatní: GA AV ČR(CZ) M200961205 Institutional support: RVO:60077344 Keywords : Sphaerosporosis * Histozoic myxozoans * Blood stages * Cyprinids * 18S rDNA * Phylogeny * In situ hybridization Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.586, year: 2013

  15. Experimental investigation of cobalt-60 accumulation and elimination in Cyprinus carpio (L.)

    International Nuclear Information System (INIS)

    When introduced in cation form, 60Co showed a distinct tendency to form anion or neutral complexes (50% and 20%, respectively); this was favorized by a high biomass content per liter of water. The ionic evolution of the radionuclide did not affect its accumulation level in the fish. Direct contamination predominated (70%) and the concentration factor at equilibrium was 10. Nutritional transfer from Limnaea to carp, after ingestion of 45 contaminated rations in 63 days, resulted in a low radionuclide retention factor (3 x 10-3) that was not subject to bioamplification. Cobalt-60 was primarily eliminated via urine (60%), while the fecal matter contained only 20% of the radioactive isotope ingested in the food. The 60Co content of carp contaminated from the water increased proportionally with the temperature. However, the 60Co concentration did not vary significantly with the thermal factor, and the radionuclide concentration factor ranged from 4 to 6 between 80C and 250C. Decontamination was generally a two-phase process characterized by a short biological half-life (60Co contained in the fish. The high 60Co content of the digestive tract may be explained by fecal excretion of the radioactive isotope (30-40% of the elimination). The kidneys act as radionuclide elimination regulators via the urinary system

  16. The effects of ciprofloxacin on early life stages of common carp (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Živná, D.; Plhalová, L.; Chromcová, L.; Blahová, J.; Prokeš, Miroslav; Skorič, M.; Maršálek, P.; Prášková, E.; Štěpánová, S.; Svobodová, Z.

    (2016). ISSN 1382-6689 Institutional support: RVO:68081766 Keywords : fluoroquinolones * embryo-larval toxicity test * antioxidant enzymes * detoxifying enzymes * lipid peroxidation Subject RIV: EG - Zoology Impact factor: 2.084, year: 2014

  17. The effects of metribuzin on early life stages of common carp (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Štěpánová, S.; Doleželová, P.; Plhalová, L.; Prokeš, Miroslav; Maršálek, P.; Škorič, M.; Svobodová, Z.

    2012-01-01

    Roč. 103, č. 2 (2012), s. 152-158. ISSN 0048-3575 Institutional support: RVO:68081766 Keywords : Fish * Embryo–larval toxicity test * LOEC * Triazine * Early development Subject RIV: EG - Zoology Impact factor: 2.111, year: 2012

  18. Effect of terbutryn at environmental concentrations on early life stages of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Velíšek, J.; Stará, A.; Máchová, J.; Dvořák, P.; Zusková, E.; Prokeš, Miroslav; Svobodová, Z.

    2012-01-01

    Roč. 102, č. 1 (2012), s. 102-108. ISSN 0048-3575 Institutional research plan: CEZ:AV0Z60930519 Keywords : Triazine * Embryo–larval toxicity test * Lowest observed-effect concentration * No observed-effect concentration * Early development * Malformation Subject RIV: GL - Fishing Impact factor: 2.111, year: 2012

  19. New metacestodes of gryporhynchid tapeworms (Cestoda: Cyclophyllidea) from carp (Cyprinus carpio Linnaeus, 1758) from Mozambique, Africa

    Czech Academy of Sciences Publication Activity Database

    Scholz, Tomáš; Boane, C.; Saraiva, A.

    2008-01-01

    Roč. 75, č. 2 (2008), s. 315-320. ISSN 1525-2647 R&D Projects: GA MŠk LC522; GA ČR GA524/08/0885 Institutional research plan: CEZ:AV0Z60220518 Keywords : cestode larvae * Gryporhynchidae * morphology * freshwater fish Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.477, year: 2008

  20. Spermatozoal Competition in Common Carp (Cyprinus carpio): What Is The Primary Determinant of Competition Success?

    Czech Academy of Sciences Publication Activity Database

    Linhart, Otomar; Rodina, Marek; Gela, David; Kocour, Martin; Vandeputte, M.

    2005-01-01

    Roč. 130, - (2005), s. 705-711. ISSN 1470-1626 R&D Projects: GA ČR GA524/03/0178; GA AV ČR IBS5045314 Institutional research plan: CEZ:AV0Z50450515 Keywords : spermatozoal competition Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.136, year: 2005

  1. Beta-Glucan induced immune modulation of wound healing in common carp (Cyprinus carpio)

    DEFF Research Database (Denmark)

    Jiménez, Natalia Ivonne Vera

    not in animals. β-glucans are commonly used as immune modulators, but the mechanisms through which the modulation is achieved remains to be understood. Wound healing and tissue regeneration are essential mechanisms to ensure the survival and health of any organism. Studies based in mammalian systems...... have shown the importance of fibroblasts, macrophages, reactive oxygen species (especially hydrogen peroxide) and certain cytokines during wound healing processes. In fish however, only a few studies have been devoted tissue regeneration and modulation of cell proliferation during wound healing, even...... hydrogen peroxide (5 μM) showed a slight increase in percentage of wound recovery, whereas high doses (300 μM) impaired wound closure and caused cell death. The results elucidated the capacity of hydrogen peroxide to influence the fate of tissue regeneration through the establishment of environments...

  2. Effect of salicylic acid on early life stages of common carp (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Živná, D.; Sehonová, P.; Plhalová, L.; Maršálek, P.; Blahová, J.; Prokeš, Miroslav; Divišová, L.; Stancová, V.; Dobšíková, R.; Tichý, F.; Široká, Z.; Svobodová, Z.

    2015-01-01

    Roč. 40, č. 1 (2015), s. 319-325. ISSN 1382-6689 Institutional support: RVO:68081766 Keywords : Pharmaceutical residues * NSAIDs * Oxidative stress * Lipid peroxidation Subject RIV: EG - Zoology Impact factor: 2.084, year: 2014

  3. Muscle to meat conversion in common carp (cyprinus carpio): new insights involving apoptosis

    OpenAIRE

    Boudida, Yasmine; Becila, Samira; GAGAOUA, Mohammed; Boudjellal, Abdelghani; Sentandreu, Miguel; Ouali, Ahmed

    2015-01-01

    Tenderization of fish muscle results in its deterioration. Different proteolytic systems exist within the muscular cell. In this decade we have integrated a new process capable to better explain the meat tenderization process namely apoptosis. This process is orchestrated by the family of cysteine aspartate-specific proteases named caspases, which are probably the first proteolytic system to be implicated in postmortem proteolysis and meat tenderization. Our study contribute to understanding ...

  4. Receptor-Mediated and Lectin-Like Activities of Carp (Cyprinus carpio) TNF-¿

    NARCIS (Netherlands)

    Forlenza, M.; Magez, S.; Scharsack, J.P.; Westphal, A.H.; Savelkoul, H.F.J.; Wiegertjes, G.F.

    2009-01-01

    Functional characterization of TNF- in species other than mammalian vertebrates is limited, and TNF- has been studied in a limited number of fish species, primarily in vitro using recombinant proteins. Studies on TNF- from different fish species so far pointed to several inconsistencies, in particul

  5. In vitro study on sperm competition in common carp (Cyprinus carpio L.)

    OpenAIRE

    Kaspar, Vojtech; Hulak, Martin; Kohlmann, Klaus; Vandeputte, Marc; Rodina, Marek; Gela, David; Linhart, Otomar

    2008-01-01

    Sperm competition occurs when sperm from different males compete for fertilization. The aim of this in vitro fertilization study was to compare the competitive success of five males using heterosperm with an equal number of sperm from each male and four different sperm/egg ratios (5,000, 10,000, 20,000 and 100,000 spermatozoa per egg) in order to better understand variations of competitive fertilization. The roles of sperm motility and velocity were studied. Fertilization and hatching rates o...

  6. Sub-lethal toxicity of chlorpyrifos on Common carp, Cyprinus carpio (Linnaeus, 1758): Biochemical response

    OpenAIRE

    Mahdi Banaee; Behzad Nematdoust Haghi; Ahmed Th. A. Ibrahim

    2014-01-01

    Chlorpyrifos, an organophosphate pesticide, is widely used to control pests in agriculture farms and orchards of fruit trees. In this study, the fish were exposed to sub-lethal concentrations of chlorpyrifos which were determined based on numerical value of 96 h LC50. Blood was sampled after 10, 20 and 30 days and biochemical parameters including glucose, total protein, albumin, globulin, triglyceride and cholesterol levels, and aspartate aminotransferase (AST), alanine aminotransferase (ALT)...

  7. Effect of deltamethrin on the biochemical profile of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Velíšek, J.; Dobšíková, R.; Svobodová, Z.; Modrá, H.; Lusková, Věra

    2006-01-01

    Roč. 76, č. 6 (2006), s. 992-998. ISSN 0007-4861 R&D Projects: GA ČR GD523/03/H076 Institutional research plan: CEZ:AV0Z60930519 Keywords : fish * metabolism * cypermethrin Subject RIV: CE - Biochemistry Impact factor: 0.505, year: 2006 http://www.springerlink.com/content/e974g87836848876/fulltext.pdf

  8. Cryopreservation of common carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues

    Czech Academy of Sciences Publication Activity Database

    Li, P.; Hulák, M.; Li, Z.; H.; Šulc, Miroslav; Pšenička, M.; Rodina, M.; Gela, D.; Linhart, O.

    2013-01-01

    Roč. 80, č. 2 (2013), s. 84-89. ISSN 0093-691X Institutional support: RVO:61388971 Keywords : Cryopreservation * Sperm * Phosphorylation Subject RIV: CE - Biochemistry Impact factor: 1.845, year: 2013

  9. Classical and molecular cytogenetics of Khawia sinensis (Cestoda: Caryophyllidea), invasive parasite of carp, Cyprinus carpio

    Czech Academy of Sciences Publication Activity Database

    Orosová, Martina; Oros, A. M.

    2012-01-01

    Roč. 110, č. 5 (2012), s. 1397-1944. ISSN 0932-0113 R&D Projects: GA MŠk LC522; GA ČR GA524/08/0885 Institutional research plan: CEZ:AV0Z60220518 Keywords : TAPEWORMS PLATYHELMINTHES * RIBOSOMAL DNA * CHROMOSOME S * EVOLUTION * FISH * RDNA * PSEUDOPHYLLIDEA * LOCATION * GENES * DIFFERENTIATION Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.852, year: 2012 http://link.springer.com/content/pdf/10.1007%2Fs00436-011-2720-x

  10. Therapeutic Effect of Phyllanthus emblica on Disease Induced Common Carp Cyprinus carpio by Aeromonas hydrophila

    Directory of Open Access Journals (Sweden)

    A. Valsa Judit Anto

    2015-01-01

    Full Text Available The fresh water fish are the important source of animal protein in human population. Fish can adapt themselves to adverse environmental conditions by changing their physiological activities. Immunostimulants like amla (Phyllanthus emblica has the additional effects such as enhancement of growth and increase in the survival rate of the fish under stress. Hematological studies on fish have assumed greater significance due to increasing emphasis on pisciculture and greater awareness on natural freshwater resources in the tropics. The present study focuses on the disease curing effect of the extracts of amla and its role on survival and mortality, opercular movement, oxygen consumption and hematological parameters, such as, leucocytes count and hemoglobin content.

  11. Effect of nitrite on early-life stages of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Kroupová, H.; Prokeš, Miroslav; Mácová, S.; Peňáz, Milan; Baruš, Vlastimil; Novotný, L.; Máchová, J.

    2010-01-01

    Roč. 29, č. 3 (2010), s. 535-540. ISSN 0730-7268. [International Workshop on Aquatic Toxicology and Biomonitoring /1./. Vodňany, 27.08.2008-29.08.2008] Institutional research plan: CEZ:AV0Z60930519 Keywords : Embryo–larval toxicity test * Lowest-observed-effect concentration * No-observed-effect concentration * Early development * Histopathology Subject RIV: DN - Health Impact of the Environment Quality Impact factor: 3.026, year: 2010

  12. Early ontogeny, growth and mortality of common carp (Cyprinus carpio) at low concentrations of dimethyl sulfoxide

    Czech Academy of Sciences Publication Activity Database

    Máchová, J.; Prokeš, Miroslav; Kroupová, H.; Svobodová, Z.; Mácová, S.; Doleželová, P.; Velíšek, J.

    2009-01-01

    Roč. 78, č. 3 (2009), s. 505-512. ISSN 0001-7213 R&D Projects: GA MZe QH71305 Grant ostatní: GA MZe(CZ) QH82117 Institutional research plan: CEZ:AV0Z60930519 Keywords : auxiliary substance * toxicity * fish embryos * larvae Subject RIV: GL - Fishing Impact factor: 0.403, year: 2009

  13. Outcomes of repeated exposure of the carp (Cyprinus carpio L.) to cyanobacteria extract

    Czech Academy of Sciences Publication Activity Database

    Palíková, M.; Navrátil, S.; Krejčí, R.; Štěrba, F.; Tichý, F.; Kubala, Lukáš; Maršálek, Blahoslav; Bláha, L.

    2004-01-01

    Roč. 73, č. 2 (2004), s. 259-265. ISSN 0001-7213 R&D Projects: GA ČR GP524/01/P027 Institutional research plan: CEZ:AV0Z5004920 Keywords : erythrocytes * leukocytes * plasma enzymes Subject RIV: BO - Biophysics Impact factor: 0.449, year: 2004

  14. Histopathology of carp (Cyprinus carpio L.) larvae exposed to Cyanobacteria extract

    Czech Academy of Sciences Publication Activity Database

    Palíková, M.; Navrátil, S.; Tichý, F.; Štěrba, F.; Maršálek, Blahoslav; Bláha, L.

    2004-01-01

    Roč. 73, - (2004), s. 253-257. ISSN 0001-7213 R&D Projects: GA AV ČR KSK6005114; GA ČR GP524/01/P027 Institutional research plan: CEZ:AV0Z6005908 Keywords : microcystins * embryo-larval test * liver damage Subject RIV: EF - Botanics Impact factor: 0.449, year: 2004

  15. The effects of diclofenac on early life stages of common carp (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Štěpánová, S.; Prášková, E.; Chromcová, L.; Plhalová, L.; Prokeš, Miroslav; Blahová, J.; Svobodová, Z.

    2013-01-01

    Roč. 35, č. 3 (2013), s. 454-460. ISSN 1382-6689 Institutional support: RVO:68081766 Keywords : Fish * Embryo–larval toxicity test * Oxidative stress * LOEC * NOEC Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.862, year: 2013

  16. Are fish immune systems really affected by parasites? an immunoecological study of common carp (Cyprinus carpio)

    OpenAIRE

    Flajšhans Martin; Tolarová Soňa; Hyršl Pavel; Morand Serge; Rohlenová Karolína; Šimková Andrea

    2011-01-01

    Abstract Background The basic function of the immune system is to protect an organism against infection in order to minimize the fitness costs of being infected. According to life-history theory, energy resources are in a trade-off between the costly demands of immunity and other physiological demands. Concerning fish, both physiology and immunity are influenced by seasonal changes (i.e. temporal variation) associated to the changes of abiotic factors (such as primarily water temperature) and...

  17. The effects of atrazine exposure on early life stages of common carp (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Chromcová, L.; Blahová, J.; Plhalová, L.; Živná, D.; Štěpánová, S.; Prášková, E.; Prokeš, Miroslav; Zelníčková, L.; Škorič, M.; Svobodová, Z.

    2013-01-01

    Roč. 34, Suppl. 2 (2013), s. 95-101. ISSN 0172-780X Institutional support: RVO:68081766 Keywords : embryo-larval toxicity test * LOEC * oxidative stress * TBARS * defense enzymes Subject RIV: EG - Zoology Impact factor: 0.935, year: 2013

  18. Effect of nitrite on early-life stage of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Kroupová, H.; Prokeš, Miroslav; Peňáz, Milan; Baruš, Vlastimil; Mácová, S.; Novotný, L.; Máchová, J.

    Vodňany: University of South Bohemia in České Budějovice, Research Institute of Fish Culture and Hydrobiology, 2008 - (Velíšek, J.; Kroupová, H.). s. 72 [International workshop on the aquatic toxicology and biomonitoring /1./. 27.08.2008-29.08.2008, Vodňany] R&D Projects: GA MZe QH71305 Institutional research plan: CEZ:AV0Z60930519 Keywords : embryo-larval toxicity test * LOEC * NOEC * early development * histology Subject RIV: EH - Ecology, Behaviour http://www.vurh.jcu.cz/images/stories/1st_workshop/abstract_book.pdf

  19. Β-glucan-induced stimulation in common carp (Cyprinus carpio) during tissue regeneration

    DEFF Research Database (Denmark)

    Jiménez, Natalia Ivonne Vera; Nielsen, Michael Engelbrecht

    cells (Macrophages) and tissue related cells (Fibroblasts) will be evaluated. Parameters as respiratory burst activity and expression profiles of immune related genes will be measured in cell cultures and in in vivo experiments. The methodology of the project involves the creation of protocols for...... extraction of damageassociated molecular patterns, handling and maintenance of cellular cultures, application of protocols for quantification of respiratory burst and the usage of real‐time quantitative PCR to quantify expression of genes of interest (IL‐1β, IL‐10, IL‐8, TNF‐α, TGF‐β, COL‐1A, MMPP9, FGFs and...... IGFs). This study will provide further understanding on the effect of β‐glucan as a modulator, which could improve fish welfare and health as well as having an economic potential for the production in aquaculture industry....

  20. 铜离子对转基因和非转基因红鲤行为的影响%Effect of Copper (II)on the Behavior of Transgenic and Non-transgenic Cyprinus carpio

    Institute of Scientific and Technical Information of China (English)

    沈佳品; 薛良义

    2015-01-01

    在构建转基因红鲤的基础上,采用生物预警系统比较和分析了非转基因和转基因红鲤(Cyprinus carpio)在不同铜离子浓度条件下的游速和活动范围的变化。转基因红鲤和非转基因红鲤孵育自同一批受精卵,转基因红鲤体长(5.31±0.64)cm、体重(3.40±0.55)g,非转基因红鲤体长(4.58±0.59)cm、体重(2.40±0.58)g。Cu-SO4的浓度设置0(对照)、5、15、25、35和45μg/L共6组。生物预警系统包括储水池、数字摄像仪和数据运算处理器,可以记录鱼的二维移动轨迹,并计算出鱼的平均游速。试验结果表明,非转基因和转基因红鲤的平均游速分别为:对照组1、1.57 BL/s,5μg/L 组1.24、1.07 BL/s,15μg/L 组1.61、1.03 BL/s,25μg/L 组1.50、1.59 BL/s,35μg/L组1.62、1.61 BL/s,45μg/L组1.25、1.97BL/s。非转基因和转基因红鲤的活动范围:除35μg/L组外,其他组的坐标X值非转基因与转基因红鲤间差异都极显著(P<0.01);对照组、5和25μg/L浓度组,转基因红鲤坐标X值极显著高于非转基因红鲤;15和45μg/L 浓度组,非转基因红鲤坐标X值极显著高于转基因红鲤。低于45μg/L时,铜离子没有对转基因和非转基因红鲤产生明显的毒性;在45μg/L浓度组,转基因红鲤对铜离子不敏感,而非转基因红鲤较敏感。%Copper is a major contaminant in water,and can affect the behavior and metabolism of fish.Cyprinus carpio is a common ornamental fish,and capable of adapting to poor quality water.To date,the effect of copper (II)on the behavior of Cyprinus carpio has not been reported.After establishing transgenic Cyprinus carpio,we compared the swimming behavior of both non-transgenic and transgenic Cyprinus carpio under different concentra-tions of copper (II)ions.The average swimming velocity (ASV)and the range of activity of non

  1. Use of oxidative stress biomarkers in Cyprinus carpio L. for the evaluation of water pollution in Ataturk Dam Lake (Adiyaman, Turkey).

    Science.gov (United States)

    Karadag, Hasan; Fırat, Özgür; Fırat, Özge

    2014-03-01

    Adiyaman city, which is located in the north of the Ataturk Dam Lake, has no wastewater purification facilities which results in municipal, agricultural, and industrial wastewater discharges directly entering the reservoir. To assess the pollution in the dam lake, we used several oxidative stress biomarkers in blood tissue of Cyprinus carpio. Fish samples were taken from Sitilce, polluted area by untreated wastewaters, and Samsat, relatively clean area, in the reservoir in August 2012. The activity of catalase and level of malondialdehyde increased while activity of superoxide dismutase and glutathione level decreased in fish from Sitilce site when compared to Samsat site. The findings of the present investigation suggest that the presence of certain prooxidative compounds that can lead to oxidative stress in the fish at the Sitilce site and oxidative stress biomarkers may be important in order to evaluate the effects of untreated wastewaters on living organisms in the dam lake. PMID:24381981

  2. Ecología poblacional de Cyprinus carpio (TELEOSTEI) en los Humedales de Ajó, Buenos Aires

    OpenAIRE

    Maiztegui, Tomás

    2016-01-01

    Este trabajo tuvo como objetivo principal conocer la biología y ecología poblacional de la carpa común (Cyprinus carpio) en los Humedales de Ajó, con el fin de aportar información que permita diseñar estrategias enfocadas en el manejo de esta especie exótica dentro de esta área protegida. La hipótesis central se basó en comprobar que la carpa común en los Humedales de Ajó ha encontrado un ambiente propicio para el desarrollo de una población local auto-sostenida y se convirtió en la especie d...

  3. Impact of low dose of organophosphate, monocrotophos on the epithelial cells of gills of Cyprinus carpio communis Linn.--SEM study.

    Science.gov (United States)

    Johal, M S; Sharma, M L; Ravneet

    2007-07-01

    The paper deals with the deleterious changes at ultrastructural level of the epithelial cells of gills of Cyprinus carpio communis Linn. upon exposure to 1/10th of LC50 of monocrotophos which is considered to be insignificant concentration from toxicological point of view. The gills of the fish are the primary corridor formolecularexchange between the internal milieu of a fish and its environment. Gills perform numerous functions such as oxygen uptake and CO2 excretion, osmoregulation, acid-basic balance, excretion of nitrogenous compounds and taste. Hazardous chemicals present in water may alter the morphology of the epithelial cells of gills of the fish, which may affect the process of diffusion of gases and ultimately the overall health of the fish. To prove this fact Cyprinus carpio communis Linn. was kept in water for 30 days having low concentration of 0.038 ppm (1\\10th of LC50) of monocrotophos and an attempt was made to study the different types of degenerations produced in the epithelial cells of gills as compared to the normal epithelial cells of gills of this culturable fish using Scanning Electron Microscope (SEM) technique. The ultrastructural changes due to the toxic exposure at finer scale were thinning of microridges, upliftment of epithelial cells, development of hyperplasia, decrease in the density of mucous cells which are considered to be the first line of defence and total dystrophy of epithelial tissue. Thus, it is opined that a low concentration of monocrotophos has the potential to bring different type of degenerations at finer scale hence affecting the fish's health drastically and altering the fitness of the fish in water even having insignificant amount of this toxicant in the ambient water PMID:18380092

  4. Estimation de la survie des alevins de carpe (C. carpio au cours de leur premier mois d'existence

    Directory of Open Access Journals (Sweden)

    MOREAU J.

    1979-10-01

    Full Text Available Des observations réalisées sur deux stations piscicoles de Madagascar, dans des étangs ne recevant ni engrais ni nourriture, révèlent qu'au cours de leur premier mois les alevins de carpe (C. carpio subissent des mortalités voi-sines de 50 %. Ces dernières sont encore plus élevées en début et en fin de saison de reproduction. Au début, les fortes mortalités sont dues à la température trop basse et aux disponibilités alimentaires insuffisantes ; en fin de saison de reproduction, la température trop élevée et la mauvaise qualité des œufs sont sans doute en cause. Une fumure adéquate des étangs de grossissement des alevins et une alimentation correcte des géniteurs permettront peut-être de diminuer ces mortalités.

  5. Feeding Cyprinus carpio with infectious materials mediates cyprinid herpesvirus 3 entry through infection of pharyngeal periodontal mucosa

    OpenAIRE

    Fournier Guillaume; Boutier Maxime; Stalin Raj Victor; Mast Jan; Parmentier Eric; Vanderwalle Pierre; Peeters Dominique; Lieffrig François; Farnir Frédéric; Gillet Laurent; Vanderplasschen Alain

    2012-01-01

    Abstract Cyprinid herpesvirus 3 (CyHV-3), also known as Koi herpesvirus, is the etiological agent of a mortal disease in common and koi carp. Recently, we investigated the entry of CyHV-3 in carp using bioluminescence imaging and a CyHV-3 recombinant strain expressing luciferase (LUC). We demonstrated that the skin is the major portal of entry after inoculation of carp by immersion in water containing CyHV-3. While this model of infection mimics some natural conditions in which infection take...

  6. Antioxidative effects of N-acetylcysteine, lipoic acid, taurine, and curcumin in the muscle of Cyprinus carpio L. exposed to cadmium

    International Nuclear Information System (INIS)

    We investigated the muscle tissue of a teleost Cyprinus carpio L. to find out whether N-acetylcysteine (NAC), alpha-lipoic acid (LA), taurine (TAU), and curcumin (CUR) were able to counteract oxidative stress induced by acute exposure to cadmium (Cd). The muscle tissue was dissected 96 h after a single intraperitoneal injection of Cd (5 mg kg-1) and of antioxidant substances (50 mg kg-1). Using spectrophotometry, we determined the glutathione redox status, lipid peroxidation levels and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione disulphide reductase (GR). Accumulation of Cd in the muscle was analysed using inductively coupled plasma - optical emission spectrometry (ICP-OES). All substances lowered Cd levels in the following order of efficiency; LA=NAC>TAU=CUR. Cadmium increased SOD activity, but CAT activity declined, regardless of antioxidant treatment. Treatment with CUR induced GPx activity. Treatment with TAU lowered Cd due to higher total glutathione (tGSH). The most effective substances on lipid peroxidation were LA and NAC due to a greater Cd-lowering potential. It seems that the protective role of TAU, LA, and NAC is not necessarily associated with antioxidant enzymes, but rather with their own activity. (authors)

  7. Effects of carbon nanomaterials fullerene C60 and fullerol C60(OH)18–22 on gills of fish Cyprinus carpio (Cyprinidae) exposed to ultraviolet radiation

    International Nuclear Information System (INIS)

    In consequence of their growing use and demand, the inevitable environmental presence of nanomaterials (NMs) has raised concerns about their potential deleterious effects to aquatic environments. The carbon NM fullerene (C60), which forms colloidal aggregates in water, and its water-soluble derivative fullerol (C60(OH)18–22), which possesses antioxidant properties, are known to be photo-excited by ultraviolet (UV) or visible light. To investigate their potential hazards to aquatic organisms upon exposure to UV sunlight, this study analyzed (a) the in vitro behavior of fullerene and fullerol against peroxyl radicals (ROO·) under UV-A radiation and (b) the effects of these photo-excited NMs on oxidative stress parameters in functional gills extracted from the fish Cyprinus carpio (Cyprinidae). The variables measured were the total antioxidant capacity, lipid peroxidation (TBARS), the activities of the antioxidant enzymes glutathione reductase (GR) and glutamate cysteine ligase (GCL), and the levels of the non-enzymatic antioxidant glutathione (GSH). The obtained results revealed the following: (1) both NMs behaved in vitro as antioxidants against ROO· in the dark and as pro-oxidants in presence of UV-A, the latter effect being reversed by the addition of sodium azide, which is a singlet oxygen (1O2) quencher; (2) fullerene induced toxicity with or without UV-A incidence, with a significant (p 1O2 generation; and (3) fullerol also decreased GCL activity and GSH formation (p 1O2 formation.

  8. Feeding Cyprinus carpio with infectious materials mediates cyprinid herpesvirus 3 entry through infection of pharyngeal periodontal mucosa

    Directory of Open Access Journals (Sweden)

    Fournier Guillaume

    2012-01-01

    Full Text Available Abstract Cyprinid herpesvirus 3 (CyHV-3, also known as Koi herpesvirus, is the etiological agent of a mortal disease in common and koi carp. Recently, we investigated the entry of CyHV-3 in carp using bioluminescence imaging and a CyHV-3 recombinant strain expressing luciferase (LUC. We demonstrated that the skin is the major portal of entry after inoculation of carp by immersion in water containing CyHV-3. While this model of infection mimics some natural conditions in which infection takes place, other epidemiological conditions could favour entry of virus through the digestive tract. Here, we investigated whether ingestion of infectious materials mediates CyHV-3 entry through the digestive tract. Carp were fed with materials contaminated with the CyHV-3 LUC recombinant (oral contamination or immersed in water containing the virus (contamination by immersion. Bioluminescence imaging analyses performed at different times post-infection led to the following observations: (i the pharyngeal periodontal mucosa is the major portal of entry after oral contamination, while the skin is the major portal of entry after contamination by immersion. (ii Both modes of inoculation led to the spreading of the infection to the various organs tested. However, the timing and the sequence in which some of the organs turned positive were different between the two modes of inoculation. Finally, we compared the disease induced by the two inoculation modes. They led to comparable clinical signs and mortality rate. The results of the present study suggest that, based on epidemiological conditions, CyHV-3 can enter carp either by skin or periodontal pharyngeal mucosal infection.

  9. Simulated population responses of common carp to commercial exploitation

    Energy Technology Data Exchange (ETDEWEB)

    Weber, Michael J.; Hennen, Matthew J.; Brown, Michael L.

    2011-12-01

    Common carp Cyprinus carpio is a widespread invasive species that can become highly abundant and impose deleterious ecosystem effects. Thus, aquatic resource managers are interested in controlling common carp populations. Control of invasive common carp populations is difficult, due in part to the inherent uncertainty of how populations respond to exploitation. To understand how common carp populations respond to exploitation, we evaluated common carp population dynamics (recruitment, growth, and mortality) in three natural lakes in eastern South Dakota. Common carp exhibited similar population dynamics across these three systems that were characterized by consistent recruitment (ages 3 to 15 years present), fast growth (K = 0.37 to 0.59), and low mortality (A = 1 to 7%). We then modeled the effects of commercial exploitation on size structure, abundance, and egg production to determine its utility as a management tool to control populations. All three populations responded similarly to exploitation simulations with a 575-mm length restriction, representing commercial gear selectivity. Simulated common carp size structure modestly declined (9 to 37%) in all simulations. Abundance of common carp declined dramatically (28 to 56%) at low levels of exploitation (0 to 20%) but exploitation >40% had little additive effect and populations were only reduced by 49 to 79% despite high exploitation (>90%). Maximum lifetime egg production was reduced from 77 to 89% at a moderate level of exploitation (40%), indicating the potential for recruitment overfishing. Exploitation further reduced common carp size structure, abundance, and egg production when simulations were not size selective. Our results provide insights to how common carp populations may respond to exploitation. Although commercial exploitation may be able to partially control populations, an integrated removal approach that removes all sizes of common carp has a greater chance of controlling population abundance

  10. The Anesthesia Effects of Clove Oil on Koi Carp (Cy prinus carpio)%丁香酚对锦鲤麻醉效果的研究

    Institute of Scientific and Technical Information of China (English)

    张志明; 胡盼; 姜志强; 刘东超; 丁慧萍

    2014-01-01

    水温18~20℃条件下,测定了丁香酚对锦鲤的麻醉效果。锦鲤体长12~15 cm ,质量30~40 g。通过预试验设计的丁香酚质量浓度梯度为10、30、60、100、200 m g/L。根据锦鲤的行为特征变化将麻醉及复苏过程各分成五个阶段。试验结果显示,随着丁香酚质量浓度30~200 mg/L的逐渐升高,锦鲤达到A4期麻醉状态的时间由32.27 min缩短至4.10 min ,复苏时间由8.26 min延长至13.38 min (P<0.01)。30 mg/L丁香酚液麻醉锦鲤60 min以及60 mg/L麻醉30 min ,锦鲤复苏率均为100%;100 mg/L麻醉20 min ,锦鲤复苏率为60%;200 mg/L麻醉10 min ,锦鲤复苏率为80%,麻醉15 min ,复苏率仅20%。经丁香酚液深麻的锦鲤置于空气中,随着丁香酚质量浓度30~200 m g/L的逐渐升高,锦鲤苏醒弹动时间由2.1 min延长至25.8 min(P<0.05)。不同质量浓度丁香酚麻醉锦鲤24 h ,丁香酚质量浓度大于35 mg/L时,锦鲤全部死亡,30 mg/L时,复苏率为60%,丁香酚质量浓度为10、15、20、25 mg/L时,复苏率均为100%。分别用5、10、15、20、25 mg/L丁香酚液麻醉锦鲤,耗氧率及排氨率均随质量浓度的升高而降低( P<0.05)。试验结果表明,锦鲤麻醉运输适宜质量浓度为15~20 m g/L ,锦鲤人工操作适宜质量浓度100 mg/L。%T he effects of clove oil (10 mg/L ,30 mg/L ,60 mg/L ,100 mg/L and 200 mg/L ) on anesthesia was investigated in koi carp (Cyprinus carpio) with body length of 12-15 cm and body weight of 30-40 g at water temperature of (18-20 ℃) .The anesthesia and recovery process were both divided into five stages according to the characteristics of the fish behavior .The anesthesia induction period was found to be shortened from 32 .27 min at 30 mg/L of clove oil to 4 .10 min at 200 mg/L of clove oil ,w hile the recovery time was found to be significantly prolonged from 8 .26min at 30 mg/L of clove oil to

  11. Supplementation of microbial levan in the diet of Cyprinus carpio fry (Linnaeus, 1758) exposed to sublethal toxicity of fipronil: effect on growth and metabolic responses.

    Science.gov (United States)

    Gupta, S K; Pal, A K; Sahu, N P; Jha, A K; Akhtar, M S; Mandal, S C; Das, P; Prusty, A K

    2013-12-01

    A 60-day feeding trial was conducted to study the effect of dietary microbial levan on growth performance and metabolic responses of Cyprinus carpio fry exposed to sublethal dose (1/10th LC₅₀) of fipronil [(±)-5-amino-1-(2,6-dichloro-α,α,α-trifluoro-p-tolyl)-4-trifluoromethylsulfinylpyrazole-3-carbonitrile]. Two hundred and twenty five fry were randomly distributed in five treatments in triplicates. Four purified diets were prepared with graded levels of microbial levan. Five different treatment groups were levan control L₀P₀ (basal feed + 0 % levan without exposure to pesticide); pesticide control L₀P₁ (basal feed + 0 % levan with exposure to pesticide); L₀.₂₅P₁ (basal feed + 0.25 % levan with exposure to pesticide); L₀.₅₀P₁ (basal feed + 0.50 % levan with exposure to pesticide); and L₀.₇₅P₁ (basal feed + 0.75 % levan with exposure to pesticide). Weight gain% and specific growth rate were significantly higher (p < 0.05) in levan fed groups compared to their non-levan fed counterpart. Highest (p < 0.05) content of ascorbic acid in muscle, liver and brain tissues was observed with higher level of dietary levan. Glucose-6-phosphate dehydrogenase activity decreased with the increasing level of dietary levan in the liver and muscle. Aspartate aminotransferase activity exhibited a second order polynomial relationship with the dietary levan, both in liver (Y = -1.001x² + 5.366x + 5.812, r² = 0.887) and muscle (Y = -0.566x² + 2.833x + 6.506, r² = 0.858) while alanine aminotransferase activity showed third order polynomial relationship both in liver (Y = 1.195x³ - 12.30x² + 35.23x + 9.874, r² = 0.879) and muscle (Y = 0.527x³ - 8.429x² + 31.80x + 8.718, r² = 0.990). Highest (p < 0.05) superoxide dismutase activity in gill was observed in the group fed with 0.75 % levan supplemented diet. Overall results indicated that dietary microbial levan at 0.75 % in C. carpio fry ameliorated the negative effects of fipronil and

  12. Observation on the Embryonic Development of Cyprinus carpio vat.color%瓯江彩鲤胚胎发育研究

    Institute of Scientific and Technical Information of China (English)

    姚俊杰; 何登菊; 赵云龙; 牟洪民; 冯亚楠; 朱俊华

    2012-01-01

    经人工催产、人工授精获得受精卵,对瓯江彩鲤(Cyprinus carpio var.color)的胚胎发育过程和各发育时期外部形态特征进行系统观察,并研究了不同温度条件下其胚胎发育的特点.结果表明,瓯江彩鲤受精卵圆球形、淡黄色、沉性卵、强黏性;卵径为1.72 mm,吸水后卵径达2.67 mm;受精后开始形成卵间隙,且植物极的大于动物极的;卵间隙形成的过程中出现卵黄的收缩,略变小.瓯江彩鲤胚胎发育过程可分为8个大的时期,水温18℃时需98 h48min出膜,水温23℃和26℃时分别需要62 h 20 min、48 h45 min出膜.不同温度条件下,瓯江彩鲤胚胎发育早期(胚盘-原肠胚)在18℃恒定水温条件下存活率最高,发育后期阶段(神经胚-出膜)在23℃恒定水温条件下存活率最高.%The fertilized eggs were obtained by injecting exterior hormone into the parent fish and using artificial propagating technology, as well as the embryonic development and its morphological character under different temperatures were systematically observed in the present work. The results indicated that mature fertilized eggs were yellowish, elliptical, and strong viscosity as a kind of deep egg. The egg had an initial average size of 1. 72 mm, and bulged to 2. 67 mm in diameter after absorbing water. Immediately after fertilization, the perivitelline space started to develop, but the vegetal pole was larger than that at the animal pole. During the perivitelline space formation, the egg yolk of Cyprinus carpio var. color slightly shrank in size. The process of embryonic development could be divided into eight principle stages. The embryonic period from fertilization to hatching, lasted 98 h 48 min at 18℃ , 62h 20min at 23℃ and 48h 45 min at 26℃. The highest survivorship of the eggs to early stages (blastoderm-gashula) occurred at 18 ℃ , but to the later stages (neurula-hatchitig) the highest survivorship occurred at 23℃.

  13. HEMATOLOGICAL BLOOD PARAMETERS OF YOUNG-OF-THE-YEAR CARPS (CYPRINIDAE REARED USING FISH RANCHING TECHNOLOGY IN THE SOUTHERN UKRAINE

    Directory of Open Access Journals (Sweden)

    Yu. Volichenko

    2015-12-01

    Full Text Available Purpose. To investigate the hematological characteristics of blood young-of-the-year carps reared using fish ranching technology in the southern Ukraine and to establish certain correlations between the main fish technical features of the studied groups of the young-of-the-year fish. Methodology. The studies were based on field and experimental methods adopted for fisheries, biochemical and statistical studies. Findings. The paper contains the data on mean fish weight, hematological and biochemical parameters of serum of young-of-the-year carps reared using fish ranching technology. Based on a comparative analysis of the obtained data, we detected significant peculiarities and qualitative difference in the absence of foam cells and basophils, which distinguish carp from the group of herbivorous fish, established significant correlations between mean fish weight and hematologic indices of blood. Marked by fish-breeding relationships with signs like weight and some parameters of blood components in all studied fish: hemoglobin in the range from 0.7858 to 0.9943, number of erythrocytes from 0.7843 to 0.9942, lymphocytes from 0.7848 to 0.9949, сholеsterol from 0.7640 to 0.9616 and triglycerides of 0.7499 in silver carp (Hypophthalmichthys molitrix and 0.9616 in common carp (Cyprinus carpio carpio. To show these relationships graphically, we used regression analysis and the obtained regression equations can give an accurate assessment of the quality of fish seeds through hematological blood parameters and mean weight. Originality. The analysis of hematological parameters of blood of young-of-the-year carps: common carp (Cyprinus carpio carpio, silver carp (Hypophthalmichthys molitrix, bighead carp (Hypophthalmichthys nobilis, grass carp (Ctenopharyngodon idella reared using fish ranching technology in the south of Ukraine was performed for the first time. Practical value. The obtained data allow scientifically recommending them as a component of

  14. Assessment of synthetic organic compounds, and endocrinology and histology of carp in Lake Mead

    Energy Technology Data Exchange (ETDEWEB)

    Bevans, H.; Goodbred, S.; Miesner, J.

    1995-12-31

    This study investigated the presence and biologic effects of synthetic organic compounds transported by Las Vegas Wash to Lake Mead National Recreation Area. Water, bottom sediment, and carp (Cyprinus carpio) were analyzed for synthetic organic compounds. Semipermeable membrane devices (SPMDs) were used to sample the water column. Blood samples were collected from Carp for analysis of sex steroid hormones and vitellogenin. Samples of external abnormalities and organs (liver, gill, kidney, small intestine, and gonad) were collected for histologic analysis. Compounds known to affect endocrine systems (PAHs, phthalate esters, PCBs, dioxins and furans) were detected in SPMD, bottom-sediment, and/or carp samples. The number and concentrations of compounds generally were greater in samples from Las Vegas Wash and Bay, than in samples from Callville Bay, a reference site in Lake Mead. High levels of PAHs detected in SPMDs from Callville Bay could indicate the existence of contaminant sources other than Las Vegas Wash.

  15. Determination of some heavy metals levels in common carp fingerlings

    Directory of Open Access Journals (Sweden)

    N.M. Abdulrahman

    2013-06-01

    Full Text Available This study was carried out at fish laboratory of Animal Production Department, Faculty of Agricultural Sciences, University of Sulaimaniya using commercial dry yeast in three concentration (0%, 3%, and 5% for 12 weeks to study their effects on concentration of some heavy metals (namely Cr, Cd, Co, Pb, Cu, Fe, Zn, and Mg of common carp fingerlings (Cyprinus carpio. The experiment was included three treatments each in three replicates (plastic tanks in which 10 fingerlings common carp of the same size and average weight (3.5 gram were stocked in each aquarium. The actual experimental feeding trials lasted three months. Results indicated that the concentrations of heavy metals differ among the treatments.

  16. Spring viraemia of carp virus: recent advances.

    Science.gov (United States)

    Ashraf, Usama; Lu, Yuanan; Lin, Li; Yuan, Junfa; Wang, Min; Liu, Xueqin

    2016-05-01

    Spring viraemia of carp is an environmentally and economically important disease affecting cyprinids, primarily common carp (Cyprinus carpio). The causative agent of this disease is Spring viraemia of carp virus (SVCV) - a member of the genus Vesiculovirus of the family Rhabdoviridae. The disease is presently endemic in Europe, America and several Asian countries, where it causes significant morbidity and mortality in affected fish. SVCV infection is generally associated with exophthalmia; abdominal distension; petechial haemorrhage of the skin, gills, eyes and internal organs; degeneration of the gill lamellae; a swollen and coarse-textured spleen; hepatic necrosis; enteritis; and pericarditis. The SVCV genome is composed of linear, negative-sense, ssRNA containing five genes in the order 3'-N-P-M-G-L-5', encoding a nucleoprotein, phosphoprotein, matrix protein, glycoprotein and RNA-dependent RNA polymerase, respectively. Fully sequenced SVCV strains exhibit distinct amino acid substitutions at unique positions, which may contribute to as-yet unknown strain-specific characteristics. To advance the study of SVCV and the control of spring viraemia of carp disease in the future, this review summarizes our current understanding of SVCV in terms of its genomic characteristics, genetic diversity and pathogenesis, and provides insights into antiviral immunity against SVCV, diagnosis of SVCV and vaccination strategies to combat SVCV. PMID:26905065

  17. NeemAzal T/S – toxicity to early-life stages of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Chromcová, L.; Blahová, J.; Živná, D.; Plhalová, L.; Casuscelli di Tocco, F.; Divišová, L.; Prokeš, Miroslav; Faggio, C.; Tichý, F.; Svobodová, Z.

    2015-01-01

    Roč. 60, č. 1 (2015), s. 23-30. ISSN 0375-8427 Institutional support: RVO:68081766 Keywords : Neemazal T/S * embryo-larval toxicity test * azadirachtin * oxidative stress * histopathology * insecticide Subject RIV: EG - Zoology Impact factor: 0.639, year: 2014

  18. Feminisation of young males of the common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during sexual differentiation

    NARCIS (Netherlands)

    Gimeno, S.; Komen, H.; Gerritsen, A.G.M.; Bowmer, T.

    1998-01-01

    In recent years, a hierarchy of techniques has become available for detecting chemicals which may cause endocrine disruption in the aquatic environment. The molecular structure of a chemical provides a first indication about estrogenic activity, i.e. their likelihood of interfering with the female h

  19. Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L

    Czech Academy of Sciences Publication Activity Database

    Li, P.; Hulak, M.; Koubek, Pavek; Šulc, Miroslav; Dzyuba, B.; Boryshpolets, S.; Rodina, M.; Gela, D.; Maňásková-Postlerová, Pavla; Pěknicová, Jana; Linhart, O.

    2010-01-01

    Roč. 74, č. 3 (2010), s. 413-423. ISSN 0093-691X Institutional research plan: CEZ:AV0Z50520701; CEZ:AV0Z5020903 Keywords : sperm quality * cryodamage * spermatozoa * seminal plasma * fish Subject RIV: EI - Biotechnology ; Bionics Impact factor: 2.045, year: 2010

  20. Toxic effects, bioconcentration and depuration of verapamil in the early life stages of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Steinbach, C.; Fedorova, G.; Prokeš, Miroslav; Grabicová, K.; Máchová, J.; Grabic, R.; Valentová, O.; Kocour Kroupová, H.

    461-462, 461-462 (2013), s. 198-206. ISSN 0048-9697 Institutional support: RVO:68081766 Keywords : Bioconcentration * Embryo-larval test * Half-life time * Heart rate * Verapamil Subject RIV: GL - Fishing Impact factor: 3.163, year: 2013

  1. CULTIVATION OF CLADOCERAN (CLADOCERA FOR INCREASING PROVISION OF YOUNG-OF-THE-YEAR CARP (CYPRINUS CARPIO WITH NATURAL FEEDS (REVIEW

    Directory of Open Access Journals (Sweden)

    A. Tuchapska

    2014-06-01

    Full Text Available Purpose. Natural feeds are important in pond fish diet because they contain all nutrients necessary for fish growth and development. The share of natural feeds in fish diet has great effect on fish growth and immunity, assimilation of artificial feeds. The main way of assured procurement of natural feeds for fish feeding at different stages of their development is artificial cultivation of aquatic organisms. However, cultivation of natural feeds is not virtually performed in aquaculture enterprises of Ukraine, therefore an analysis of available data on zooplankton cultivation is important for looking for optimal and economically profitable methods of enrichment of pond fish diet with natural feeds. Methodology. Methods of cladoceran cultivation were the object of the study, material for the study – literature data on ways and methods of zooplankton cultivation. Findings. Cultivation of various species of zooplankton is performed for feeding of pond fish on different life stages. Main object of cultivation in aquaculture is Daphnia magna Straus, juvenile forms of which are consumed by fish larvae, while adult organisms are the most valuable for yearlings and older fish. The efficiency of hydrobiont cultivation highly depends on the selected object, containers, where cladocerans are cultivated, optimum conditions, peculiarities of water supply, species, and application of fertilizers and feeds. Originality. The highest production of zooplankton can be obtained when cultivating D. magna in tanks with continuous flow and in net cages installed in ponds under condition of ensuring requirements of the culture in bacterial and algae feeds (due to application of fertilizers and feeding with feeds and microalgae taking into account their presence in water, which is in the tank-cultivator. Practical value. Simplicity of the methods and high efficiency of zooplankton cultivation for preparation of pond fish juveniles is the basis of its wide use in current economical conditions. Cladoceran cultivation is especially important during the period of its active consumption by fish in summer months, when a depletion of the natural forage base occur in ponds.

  2. Plasma lactate and stress hormones in common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) during stepwise decreasing oxygen levels

    NARCIS (Netherlands)

    Vianen, GJ; Van den Thillart, GEEJM; Van Kampen, M; Van Heel, TI; Steffens, AB

    2001-01-01

    By measuring the lactate response it is possible to determine whether a teleost is able to adapt to a certain oxygen level. It is hypothesized that recovery will occur at oxygen levels above the critical oxygen level (PO2)(crit) reflected by a transient lactate increase. In contrast, continuous lact

  3. The accumulation of substances in Recirculating Aquaculture Systems (RAS) affects embryonic and larval development in common carp Cyprinus carpio

    NARCIS (Netherlands)

    Martins, C.I.; Pristin, M.G.; Ende, S.S.W.; Eding, E.H.; Verreth, J.A.J.

    2009-01-01

    The accumulation of substances in Recirculating Aquaculture Systems (RAS) may impair the growth and welfare of fish. To test the severity of contaminants accumulated in RAS, early-life stages of fish were used. Ultrafiltered water from two Recirculating Aquaculture Systems (RAS), one RAS with a high

  4. New data on Myxobolus longisporus (Myxozoa: Myxobolidae), a gill infecting parasite of carp, Cyprinus carpio haematopterus, from Chinese lakes

    Czech Academy of Sciences Publication Activity Database

    Dyková, Iva; Fiala, Ivan; Nie, P.

    2003-01-01

    Roč. 50, č. 4 (2003), s. 263-268. ISSN 0015-5683 R&D Projects: GA MŠk ME 424 Grant ostatní: National Natural Science Foundation of China(CN) 30025035 Institutional research plan: CEZ:AV0Z6022909; CEZ:MSM 123100003 Keywords : Myxozoa * Myxobolus longisporus * SSU rDNA Subject RIV: EA - Cell Biology Impact factor: 0.469, year: 2003

  5. Vitellogenesis in Archigetes sieboldi Leuckart, 1878 (Cestoda, Caryophyllidea, Caryophyllaeidae), an intestinal parasite of carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Bruňanská, M.; Mackiewicz, J. S.; Nebesářová, Jana

    2012-01-01

    Roč. 27, č. 12 (2012), s. 1611-1620. ISSN 0213-3911 Institutional support: RVO:60077344 Keywords : Caryophyllidea * Archigetes sieboldi * vitellogenesis * Ultrastructure Subject RIV: EA - Cell Biology Impact factor: 2.281, year: 2012

  6. Modulation of cytochrome P450 and induction of DNA damage in Cyprinus carpio exposed in situ to surface water treated with chlorine or alternative disinfectants in different seasons.

    Science.gov (United States)

    Canistro, Donatella; Melega, Simone; Ranieri, Dario; Sapone, Andrea; Gustavino, Bianca; Monfrinotti, Monica; Rizzoni, Marco; Paolini, Moreno

    2012-01-01

    Epidemiological studies have shown an association between consumption of disinfected drinking water and adverse health outcomes. The chemicals used to disinfect water react with occurring organic matter and anthropogenic contaminants in the source water, resulting in the formation of disinfection by-products (DBPs). The observations that some DBPs are carcinogenic in animal models have raised public concern over the possible adverse health effects for humans. Here, the modulation of liver cytochrome P450-linked monooxygenases (MFO) and the genotoxic effects in erythrocytes of Cyprinus carpio fish exposed in situ to surface drinking water in the presence of disinfectants, such as sodium hypochlorite (NaClO), chlorine dioxide (ClO(2)) and peracetic acid (PAA), were investigated in winter and summer. A complex induction/suppression pattern of CYP-associated MFOs in winter was observed for all disinfectants. For example, a 3.4- to 15-fold increase was recorded of the CYP2B1/2-linked dealkylation of penthoxyresorufin with NaClO (10 days) and PAA (20 days). In contrast, ClO(2) generated the most notable inactivation, the CYP2E1-supported hydroxylation of p-nitrophenol being decreased up to 71% after 10 days' treatment. In summer, the degree of modulation was modest, with the exception of CYP3A1/2 and CYP1A1 supported MFOs (62% loss after 20 days PAA). The micronucleus (MN) induction in fish circulating erythrocytes was also analysed as an endpoint of genotoxic potential in the same fish population. Significant increases of MN induction were detected at the latest sampling time on fish exposed to surface water treated with chlorinate-disinfectants, both in winter (NaClO) and summer (NaClO and ClO(2)), while no effect was observed in fish exposed to PAA-treated water. These results show that water disinfection may be responsible for harmful outcomes in terms of MFO perturbation and DNA damage; if extrapolated to humans, they ultimately offer a possible rationale for the

  7. 鲢、鲤和鲫肝细胞原代培养%PRIMARY HEPATOCYTE CULTURE OF HYPOPHTHALMICHTHYS MOLITRIX,CYPRINUS CARPIO AND CARASSIUS AURATUS

    Institute of Scientific and Technical Information of China (English)

    李效宇; 刘永定; 宋立荣

    2001-01-01

    @@微囊藻毒素(Microcystins)是一类淡水水体中危害很严重的生物毒素(Biotoxin),由微囊藻毒素所引发的环境问题及其对人类健康的危害正日益受到科学家的关注[1]。已知微囊藻毒素作用的靶器官为肝脏,以往的研究多集中在微囊藻毒素对动物肝脏组织的损伤,如口服或腹腔注射毒素,引起肝组织结构破坏、肝出血甚至肝坏死,但用整体实验动物或器官研究微囊藻毒素毒理学较难深入,因此建立毒理学实验模型十分重要。肝脏作为动物体内最重要的解毒器官,是研究微囊藻毒素毒理学的主要对象。一般毒理学实验都采用肝脏原代培养细胞,因为原代培养细胞生理生化及遗传特性稳定,适于研究外界毒物的毒性、毒理及肝细胞对毒物的应答和解毒机理。本实验通过对鲢(Hypophthalmichthy molitrix Carier et Valencienines)、鲤(Cyprinus carpio Linnaeus)和鲫(Carassius auratus Linnaeus)肝脏原代细胞培养,以建立稳定的毒理学实验模型,为微囊藻毒素毒理学研究奠定基础。

  8. Sterile 'Judas' carp--Surgical sterilisation does not impair growth, endocrine and behavioural responses of male carp.

    Science.gov (United States)

    Patil, Jawahar G; Purser, G J; Nicholson, A M

    2015-09-15

    Use of 'Judas' fish to betray the locations of conspecifics is a powerful tool in management of invasive pest fish but poses a risk of contributing to recruitment. Our aim therefore was to generate surgically sterilised male common carp (Cyprinus carpio) and test whether they readily assimilate into wild populations, retain sexual behaviour and successfully betray the locations of feral carp. Male common carp were surgically sterilised (n=44) adopting a two-point nip technique, using either a haemoclip, suture or electro cautery to tie each of the testicular ducts about 2.5 cm cranial to urogenital sinus-retaining all of the glandular testis tissue. Observed survival (95%) and success (>70%) rates were relatively high. Plasma steroids (11-keto testosterone and 17β-estradiol) were quantified by immunoassay. A subset of sterile and control male fish (n=7 each) were implanted with radio-transmitters and released into Lake Sorell (50 km(2)) and their ability to betray the location of feral carp was assessed by radio tracking and targeted fishing. There was a statistically significant difference in 11-keto testosterone and 17β-estradiol levels over time (P0.05), implying that surgery did not compromise the animals physiologically. The sterile Judas fish integrated well into the population-behaving similarly to control Judas males and assisted in the capture of feral carp. The study marks a significant breakthrough in the management of this pest fish with potential adoption to the management of other pest fish globally. PMID:25776462

  9. Histological Investigations on an Intestinal Level in Common Carp, One Summer Old, Fed with Organic Selenium (Selplex) Supplemented Fodder

    OpenAIRE

    Aurel Şara; Alina Rodica Ani (Toma); Florin Molnar; Erol Gabor

    2011-01-01

    The goal of this research was to analyze the effect of organic Selenium (SelPlex) on the intestinal mucosa and theimpact on the growth and consumption performances in one summer old common carp (Cyprinus carpio), Lausitzand Galitian varieties. The research were conducted on a number of 392 fish, Lausitz variety divided in 2 groups andon 192 fish, Galitian variety, divided into 2 groups. The fish were raised in 2 ponds (one pond for the control groupand one pond for the experimental group), in...

  10. Inhibition Effect of Eugenol on Na+-K+-ATPase Activity in Brain and Spinal Cord of Cyprinus carpio%丁香酚对鲤鱼脑和脊髓中钠钾泵活性的抑制效果

    Institute of Scientific and Technical Information of China (English)

    孙文渊; 吕世明; 谭艾娟; 林艳红; 安苗; 华夏; 李博岩; 焦亚琴

    2015-01-01

    为探明钠钾泵(Na+-K+-ATP 酶)是否为丁香酚作用的靶位之一,以了解丁香酚的作用机制和进一步开发利用提供参考,将体重900~1000 g、体长40~45 cm 的鲤鱼随机分为对照组(10尾,不含丁香酚乳剂的水溶液)和丁香酚处理组(30尾),测定其不同麻醉时期(诱导期、麻醉期和恢复期)鲤鱼大脑、中脑、间脑、小脑、延脑和脊髓中 Na+-K+-ATP 酶的活性。结果表明:经丁香酚麻醉后不同麻醉时期鲤鱼各脑和脊髓中 Na+-K+-ATP 酶活性均不同程度下降,丁香酚的抑制作用随麻醉程度加深而加强,在麻醉期时抑制作用最强,与对照组比,丁香酚处理组各脑和脊髓中酶活性在麻醉期性均极显著下降(P <0.01);与麻醉期比,恢复期除间脑和延脑外,其余各脑组织和脊髓中酶活性均呈极显著升高(P <0.01)。在麻醉的各时期丁香酚对鲤鱼各脑和脊髓中的 Na+-K+-ATP 酶有明显的抑制作用,对鲤鱼的麻醉作用可能与其抑制脑 Na+-K+-ATP 酶的活性有关。%The Na+-K+-ATPase activity in brain, midbrain, interbrain, cerebellum, medulla oblongata and spinal cord of Cyprinus carpio treated with eugenol at different anesthesia stages (induction stage,narcosis stage and restoration stage)was detected to probe the effect of eugenol on Na+-K+-ATPase activity and provide a reference for further development and utilization of eugenol.The results showed that the Na+-K+-ATPase activity in brain,midbrain,interbrain,cerebellum,medulla oblongata and spinal cord of C.carpio treated with eugenol reduces at different anesthesia stages to varying degrees, and the inhibition effect of eugenol increases with increase of anesthesia degree.That is,the inhibition effect of eugenol is the maximum at narcosis stage.The Na+-K+-ATPase activity in brain,midbrain, interbrain,cerebellum,medulla oblongata and spinal cord of Cyprinus carpio Linnaeus

  11. Mezidruhové a sezónní změny ve struktuře pokožky hybridních jedinců karasa stříbřitého (Carassius gibelio) a kapra obecného (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Halačka, Karel; Vetešník, Lukáš

    Brno : Mendelova univerzita, 2014 - (Kopp, R.), s. 178-179 ISBN 978-80-7509-153-6. [65 let výuky rybářství na Mendelově univerzitě v Brně. Brno (CZ), 02.12.2014-03.12.2014] R&D Projects: GA ČR GAP505/12/0375 Institutional support: RVO:68081766 Keywords : Carassius gibelio * Cyprinus carpio Subject RIV: EG - Zoology

  12. Responses of biomarkers of a standardized (Cyprinus carpio) and a native (Pimelodella laticeps) fish species after in situ exposure in a periurban zone of Luján river (Argentina).

    Science.gov (United States)

    Scarcia, Paola; Calamante, Gabriela; de la Torre, Fernando

    2014-05-01

    The Luján River basin, which is located in the northwest area of the province of Buenos Aires, Argentina, receives different anthropogenic inputs before reaching the Río de la Plata estuary. The aim of this study was to assess the adverse impact of the river in the middle part of the basin. To this end, an in situ cage assay was conducted in two sites of the river (S1 and S2) near Luján city, and the responses of hepatic biomarkers of both a standardized (Cyprinus carpio) and a native (Pimelodella laticeps) species were evaluated. The biomarkers studied were the condition factor and liver somatic indices (LSI), the enzymatic activities of catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST), lipid peroxidation levels (thiobarbituric acid reactive substances, TBARS) and the induction of hepatic cytochrome P450 1A (CYP1A) and vitellogenin (Vtg) proteins. After 14 days, LSI and GST activity increased, and TBARS levels decreased in both species exposed at S1 and S2. In addition, exposure at both sites promoted an increase in SOD activity and CYP1A induction in C. carpio, while Vtg expression was observed only at S1. A shorter exposure period (7 days) caused an initial response only at S2 mediated only by CAT in P. laticeps. Finally, our results demonstrate that a 14-day period of in situ exposure in Luján River could lead to antioxidant and biotransformation processes in C. carpio and to phase II biotransformation responses in P. laticeps. PMID:22610875

  13. Tracing the biotransformation of polychlorinated biphenyls (PCBs) in common carp (Cryprinus carpio): Enantiomeric fraction and compound-specific stable carbon isotope analyses.

    Science.gov (United States)

    Tang, Bin; Luo, Xiao-Jun; Zeng, Yan-Hong; Sun, Run-Xia; Chen, Hua-Shan; Li, Zong-Rui; Mai, Bi-Xian

    2016-09-01

    Metabolites of polychlorinated biphenyls (PCBs) in fish are difficult to detect in vivo due to the complexity of biometabolism. In the present study, atropisomeric fraction analysis of chiral PCB congeners and compound-specific isotopic analysis (CSIA) were applied to trace the biotransformation of PCBs in fish by exposure of common carp (Cryprinus carpio) to the commercial PCB mixture Aroclor 1242. Stereoselective elimination of the chiral PCB congeners 91, 95, and 136 was observed, indicating a stereoselective biotransformation process. The δ(13)C values of PCBs 5/8, 18, and 20/33 in fish were increased compared with those in the spiked food, while PCBs 47/48 and 49 showed significant heavy isotope depletion. These results suggested a significant biotransformation of the corresponding individual PCB congeners although the potential PCB metabolites, hydroxylated PCBs (OH-PCBs) and methylsulfone PCBs (MeSO2-PCBs), were not detected in the fish tissue samples throughout this experiment. The results of the present study demonstrated that a combination of chiral analysis and CSIA is a promising new approach for investigating the biotransformation of PCBs in biota. PMID:27341148

  14. RESEARCHES REGARDING THE TECHNOLOGICAL PERFORMANCES OF CARP REARING DURING WINTER PERIOD IN THE CONDITIONS OF A RECIRCULATING AQUACULTURE SYSTEM

    Directory of Open Access Journals (Sweden)

    G. STEFAN

    2013-12-01

    Full Text Available The techniques of carp culture are highly diversified, ranging from the extensive production in pond or open water with no fertilization or supplemental feeding to highly intensive systems in concrete tanks or cages. Among the different carp species, common carp is the best species reared in intensive monoculture, the others (Chinese and Indian carps being usually cultivated in polyculture (P. Kestemont, 1995. An experiment was conducted in inside recirculation system conditions to identify the technological performances on carp growth and survival at the Fishing and Aquaculture Department, Galati, during winter period (February, 2007 – March, 2007. The 1-year-old carp (Cyprinus carpio 4792g; 4594 g; 4561 g and 4525 g (total weight grew to 7384g; 7017g; 6924g and 7125 g in 44 days in aquarium 1, 2, 3 and 4, respectively. In all aquariums, the fish appeared healthy and no mortality was observed. Feed conversion efficiencies (FCE had similar values among all aquariums, the highest FCE being found in B4 aquarium with 1, 57 value. Water quality parameters were acceptable range for fish culture. Results show that the carp rearing during winter period in the inside recirculation system is a very good economic solution.

  15. ABUNDANCE OF FOUR EXOTIC FISH SPECIES CYPRINUS CARPIO, CTENOPHARYNGODON IDELLA, HYPOPHTHALMICHTHYS MOLITRIX AND ARISTICHTHYS NOBILIS IN THE URPOD BEEL OF GOALPARA DISTRICT OF ASSAM

    OpenAIRE

    Rita Choudhury; Puranjit Das; Umesh C.Goswami

    2013-01-01

    The present study was carried out to find out the abundance of four exotic fish namely, common carp, grass carp, silver carp and bighead carp in the Urpod beel of Goalpara district of Assam. The Urpod beel has a rich ichthyofaunal diversity and available almost all native fish species of Assam. During last few years the beel become habitat of some exotic fish species and their numberhas been increasing day by day, causes disturbances to the native fish species. The rate of exotic fish infesta...

  16. Effects of M-cresol on Activity of Total ATPase During the Early Development of Cyprinus carpio var.color%间甲酚对瓯江彩鲤早期发育总 ATP 酶活性的影响

    Institute of Scientific and Technical Information of China (English)

    朱俊华; 梁琍; 姚俊杰; 冯亚楠

    2014-01-01

    Activity of total adenosine triphosphatase ( ATPase) was determined during the early development of Cyp-rinus carpio var.color exposed to 7 mg/L, 13 mg/L and 19 mg/L m-cresol at the temperature of (20 ±2)℃.The influence of m-cresol on the total ATPase was analyzed through biochemical and static toxicological methods .The results showed that the activity of total ATPase had no significant difference in the fertilized eggs and the mature eggs of Cyprinus carpio var.color( P>0.05) .However, the activities of total ATPase in all treatment groups dem-onstrated obvious dose-effect relationship and stage-effect relationship in response to m-cresol during the early de-velopment of Cyprinus carpio var.color.In general, the activity of total ATPase decreased with the extension of de-velopmental time and increasing of m-cresol concentration from gastrula stage of the early development of Cyprinus carpio var.color.%在水温(20±2)℃条件下,设置7 mg/L、13 mg/L、19 mg/L的间甲酚(m-cresol)浓度梯度,研究了瓯江彩鲤(Cyprinus carpio var.color)早期发育过程中总ATP酶活性变化及间甲酚对总ATP酶影响。结果表明,瓯江彩鲤总ATP酶活性在成熟卵和受精卵中无显著差异(P>0.05);各浓度组总ATP酶活性表现为良好的剂量-效应和发育时期-效应关系;总体上看,瓯江彩鲤早期发育从原肠胚期开始,总ATP酶活性随着发育时间的延长和暴露浓度的增加而降低。

  17. Induced artificial androgenesis in common tench, Tinca tinca (L., using common carp and common bream eggs

    Directory of Open Access Journals (Sweden)

    Dariusz Kucharczyk

    2014-03-01

    Full Text Available This study presents artificial induction using tench eggs, Tinca tinca (L., of androgenetic origin. The oocytes taken from common bream, Abramis brama (L. and common carp, Cyprinus carpio L. were genetically inactivated using UV irradiation and then inseminated using tench spermatozoa. Androgenetic origin (haploid or diploid embryos was checked using a recessive colour (blond and morphological markers. The percentage of hatched embryos in all experimental groups was much lower than in the control groups. All haploid embryos showed morphological abnormalities, which were recorded as haploid syndrome (stunted body, poorly formed retina, etc.. The optimal dose of UV irradiation of common bream and common carp eggs was 3456 J m–2. At this dose, almost 100% of haploid embryos were produced at a hatching rate of over 6%. Lower UV-ray doses affected abnormal embryo development. The highest yield of tench androgenesis (about 2% was noted when eggs were exposed to thermal shock 30 min after egg activation.

  18. Determination of Heavy Metal (Cu, Pb And Zn Concentrations in Muscle Tissue of Hypophthalmichthys Molitrix, Cyprinus Carpio and Ctenopharyngodon Idella Caught From Zarivar Wetland, Western Iran

    Directory of Open Access Journals (Sweden)

    Seyed Milad Jafari

    2014-12-01

    Full Text Available The present research report the heavy metal (Cu, Pb and Zn contamination in muscle tissue of three species of fish (silver carp, common carp and grass carp caught from Zarivar international wetland. Heavy metal concentrations (μg g−1, wet weight; means±S.D. in silver carp, common carp and grass carp muscle tissue were: Cu- 0.006 ±0.005, 0.01±0.008 and 0.013.91±0.011, Pb- 0.008±0.006, 0.006±0.003 and 0.011±0.007, Zn- 0.013±0.009, 0.017±0.014 and 0.009±0.008, respectively. Comparative evaluation of these metals in different fish species showed that the average concentrations of Cu, Pb and Zn of all species is significantly lower than adverse level for the species themselves and for human consumption when compared with FAO/WHO permissible limits. Therefore, their contribution to the total body burden of these heavy metals can be considered as negligibly small.

  19. ABUNDANCE OF FOUR EXOTIC FISH SPECIES CYPRINUS CARPIO, CTENOPHARYNGODON IDELLA, HYPOPHTHALMICHTHYS MOLITRIX AND ARISTICHTHYS NOBILIS IN THE URPOD BEEL OF GOALPARA DISTRICT OF ASSAM

    Directory of Open Access Journals (Sweden)

    Rita Choudhury

    2013-02-01

    Full Text Available The present study was carried out to find out the abundance of four exotic fish namely, common carp, grass carp, silver carp and bighead carp in the Urpod beel of Goalpara district of Assam. The Urpod beel has a rich ichthyofaunal diversity and available almost all native fish species of Assam. During last few years the beel become habitat of some exotic fish species and their numberhas been increasing day by day, causes disturbances to the native fish species. The rate of exotic fish infestation in the beel was determined through field investigation and catch record from January 2011 to December 2011 in the fish landing centres of the beel. Abundance Index was calculated for the four exotic species from each catch data. A marked seasonal variation of the fish catch of native and exotic fishes was noticed. Among the four exotic species common carp recorded highest catch (4.99% of total catch and bighead recorded lowest (2.84% of total catch.

  20. A dense genetic linkage map for common carp and its integration with a BAC-based physical map.

    Directory of Open Access Journals (Sweden)

    Lan Zhao

    Full Text Available BACKGROUND: Common carp (Cyprinus carpio is one of the most important aquaculture species with an annual global production of 3.4 million metric tons. It is also an important ornamental species as well as an important model species for aquaculture research. To improve the economically important traits of this fish, a number of genomic resources and genetic tools have been developed, including several genetic maps and a bacterial artificial chromosome (BAC-based physical map. However, integrated genetic and physical maps are not available to study quantitative trait loci (QTL and assist with fine mapping, positional cloning and whole genome sequencing and assembly. The objective of this study was to integrate the currently available BAC-based physical and genetic maps. RESULTS: The genetic map was updated with 592 novel markers, including 312 BAC-anchored microsatellites and 130 SNP markers, and contained 1,209 genetic markers on 50 linkage groups, spanning 3,565.9 cM in the common carp genome. An integrated genetic and physical map of the common carp genome was then constructed, which was composed of 463 physical map contigs and 88 single BACs. Combined lengths of the contigs and single BACs covered a physical length of 498.75 Mb, or around 30% of the common carp genome. Comparative analysis between common carp and zebrafish genomes was performed based on the integrated map, providing more insights into the common carp specific whole genome duplication and segmental rearrangements in the genome. CONCLUSION: We integrated a BAC-based physical map to a genetic linkage map of common carp by anchoring BAC-associated genetic markers. The density of the genetic linkage map was significantly increased. The integrated map provides a tool for both genetic and genomic studies of common carp, which will help us to understand the genomic architecture of common carp and facilitate fine mapping and positional cloning of economically important traits for

  1. Draining and liming of ponds as an effective measure for containment of CyHV-3 in carp farms.

    Science.gov (United States)

    Flamm, Agnes; Fabian, Marc; Runge, Martin; Böttcher, Kerstin; Bräuer, Grit; Füllner, Gert; Steinhagen, Dieter

    2016-08-01

    Infections of common carp Cyprinus carpio and koi, its coloured morphotypes, with the cyprinid herpesvirus 3 (CyHV-3) can induce severe clinical signs and increased mortality in affected stocks. This may significantly challenge the economic basis of carp farming in Central Europe. To limit virus spread in carp farms, effective disinfection measures for ponds stocked with infected populations are required. In the traditional European pond aquaculture of carp, draining and liming of ponds with quicklime (CaO) up to pH 12 is a well-established disinfection measure against various pathogens. The present field study investigated whether these measures are sufficient for the inactivation of CyHV-3 infectivity in carp ponds. After draining and liming, the ponds were stocked with carp fry from a CyHV-3-negative stock, and 2 ponds were examined for the presence of CyHV-3-specific DNA sequences during the growth period of the carp and in the harvested stock. Wild fish (from the ponds, and feeder and drainage canals) and water samples (from the ponds) were also examined for CyHV-3-specific DNA sequences; and naïve carp were cohabited with wild fish, or exposed to the pondwater samples, to test for the presence of infectious virus. All examined samples remained negative for CyHV-3 throughout the study. This indicates that draining and liming with quicklime can be a suitable disinfection measure for ponds after a CyHV-3 outbreak in carp aquaculture. PMID:27503923

  2. WATER BLOOM OF BLUEGREEN ALGE IN CARP FISHPOUNDS

    Directory of Open Access Journals (Sweden)

    Melita Mihaljević

    1996-03-01

    Full Text Available The massive development of bluegreen algae (Cyanophyta/Cyanobacteria, the so--called water bloom, is a frequent phenomenon in fishpond ecosystems. This study analyses water bloom development in three carp fishponds owned by a fishbreeding company at Donji Miholjac (Croatia, where one-year-old carps (Cyprinus carpio , were bred in defferent fishstock densities. Analyses of physicallychemical properties of water and phytoplankton biomass were per- formed in fortnight intervals from May till October, 1992. In all there investigated fishponds the water bloom of bluegreen algae developed, but at a different time and showing a different qualitative composition. In the fishpond with fishstock density of 250 kg/ha water bloom consisted of the species Aphanizomenon flos-aquae, and the biggest biomass (131.92 mg/I was found in August. In the fishpond with fishstock density of 437 kg/ha a water bloom consisting of species from the genues Anabaena and species Aphanizomenon flos-aquae developed at the end of July. In the fishpond with the so--called intensive breeding (fishstock density of 750 kg/ha water bloom of the species Microcystis aeruginosa developed as late as September. The beginning of water bloom development was caused by the low value (lower than 7 of the ratio between the quantities of total phosphorus and total nitrogen. However, the qualitative composition of water bloom was influenced by one-year-old carp fingerlings density.

  3. Růst a distribuce kapra obecného (Cyprinus carpio) v ÚN Brno a Dalešice

    Czech Academy of Sciences Publication Activity Database

    Prokeš, Miroslav; Mareš, J.; Baruš, Vlastimil; Habán, V.; Peňáz, Milan

    Brno : MZLU, 2008 - (Kopp, R.), s. 180-186 ISBN 978-80-7375-246-0. [Česká ichtyologická konference /11./. Brno (CZ), 03.12.2008-04.12.2008] R&D Projects: GA MZe QH71305 Institutional research plan: CEZ:AV0Z60930519 Keywords : common carp Subject RIV: GL - Fishing http://www.rybarstvi.eu:80/

  4. Induction of gynogenesis in red crucian carp using spermatozoa of blunt snout bream

    Institute of Scientific and Technical Information of China (English)

    SUN Yuandong; LIU Yun; TAO Min; LIU Shaojun; ZHANG Chun; DUAN Wei; SHEN Jiamin; WANG Jing; ZENG Chen; LONG Yu

    2007-01-01

    Gynogenetic diploid was induced in red crucian carp (RCC) ( Carassius auratus Red Variety) eggs using UV-irradiated spermatozoa from blunt snout bream (B) (Megalobrama amblycephala ) or from mirror carp (C) (Cyprinus carpio. L). Spermatozoa were genetically inactivated by an appropriate UV dosage, and then the maternal DNA was duplicated with cold shock at 0-4 ℃. When using the spermatozoa of B, the fertilization rate, hatching rate and survival at first feeding were 52.6 ± 3.0 %, 23.6 ± 4.1 % and 15.7 ± 3.4 %, respectively, and the survival at first feeding was significantly higher than that ( 11.3 ± 2.2 % ) when using the spermatozoa of C (Cyprinus carpio. L). According to the morphological characteristics, the chromosome number and the degree of gonadal development, gynogenetic RCC could be distinguished from the control hybrids of RCC♀ × B ♂ . The individuals with red body color, 100 chromosomes and normal gonadal development were successful gynogenetic RCC, while the individuals with 124 or 148 chromosomes and delayed gonadal development were hybrids of (RCC × B). The triploid hybrids (RCC × B) (2 years old) were sterile, but the tetraploid hybrids (RCC × B) were sexually mature age of two. In the present study, compared to the spermatozoa of C, the advantages of spermatozoa of B as the activation source were that could increase the survival at first feeding of gynogenetic individuals and simplify the confirmation of gynogenetic status, which suggested that the spermof B was an effective activation source for inducing gynogenesis in crucian carp.

  5. Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp

    Science.gov (United States)

    Zhong, Zhaomin; Niu, Pengfei; Wang, Mingyong; Huang, Guodong; Xu, Shuhao; Sun, Yi; Xu, Xiaona; Hou, Yi; Sun, Xiaowen; Yan, Yilin; Wang, Han

    2016-01-01

    The common carp (Cyprinus carpio) as one of the most important aquaculture fishes produces over 3 million metric tones annually, approximately 10% the annual production of the all farmed freshwater fish worldwide. However, the tetraploidy genome and long generation-time of the common carp have made its breeding and genetic studies extremely difficult. Here, TALEN and CRISPR-Cas9, two versatile genome-editing tools, are employed to target common carp bone-related genes sp7, runx2, bmp2a, spp1, opg, and muscle suppressor gene mstn. TALEN were shown to induce mutations in the target coding sites of sp7, runx2, spp1 and mstn. With CRISPR-Cas9, the two common carp sp7 genes, sp7a and sp7b, were mutated individually, all resulting in severe bone defects; while mstnba mutated fish have grown significantly more muscle cells. We also employed CRISPR-Cas9 to generate double mutant fish of sp7a;mstnba with high efficiencies in a single step. These results demonstrate that both TALEN and CRISPR-Cas9 are highly efficient tools for modifying the common carp genome, and open avenues for facilitating common carp genetic studies and breeding. PMID:26976234

  6. Effects of farm and commercial inputs on carp polyculture performance: participatory trial in an experimental field station

    Directory of Open Access Journals (Sweden)

    Martha Hernández

    2014-07-01

    Full Text Available The carp polyculture production system is the most widely used system by small-scale fish farmers in southern Brazil (States of Paraná, Santa Catarina and Rio Grande do Sul. The aim of this study was to compare biotechnical and economic parameters between a farm system (FS using farm inputs (chicken manure, maize and grass and a commercial system (CS using commercial inputs (triple super phosphate, ammonium nitrate and balanced food feeding to apparent satiation. The experiment was carried out for 196 days in earthen ponds of 500 m², with three replicates per system. The stocking density was 2,000 fish ha-1, consisting of 35% grass carp (Ctenopharyngodon idella, 30% of mirror common carp (Cyprinus carpio var. specularis, 20% of bighead carp (Aristichthys nobilis and 15% silver carp (Hypophthalmichthys molitrix. In both systems, a low level of total phosphorus in water (0.06 mg L-1 and in the sediment (4 mg L-1 was observed. Production was significantly increased in the CS (76 kg 500 m(-2196 days-1 than in the FS (43 kg 500 m-2 196 days-1. Costs and revenues were higher in the CS and profits were similar in the two systems. Similar profits do not mean that CS is necessarily more convenient. Advantages and drawbacks for small-scale farmers considering labor, land and availability of money are discussed.

  7. AMELIORATION EFFECT OF PROBIOTICS (CHEESE AND PREBIOTIC (GARLIC ON AFLATOXIN B1 INDUCED HEMATOLOGICAL ALTERATIONS IN FRESH WATER FISH Cyprinus carpio L.

    Directory of Open Access Journals (Sweden)

    J.A.PRADEEPKIRAN

    2016-07-01

    Full Text Available The objective of this study was to examine protective effect of probiotics against aflatoxin B1 (AFB1 on hematological and serum parameters which include the RBC, WBC, albumins, globulins, serum creatinine, and ALT and AST of fresh water fish Cyprinous carpio L. The total hemobiochemical analysis was compared with the AFB1 induced and probiotics (garlic, cheese treated groups. Cyprinous carpio L. (40 ± 10 g, were randomly divided into five experimental groups (15 fish per group. Group T1 represented the negative control fed with normal diet, and T2 was the positive control group fed with AFB1 contaminated diet. Groups T3, T4 were fed with AFB1-contaminated diet 200ppb supplemented with 2 mg/kg cheese, 2 mg/kg garlic, and Group T5 fed with AFB1-contaminated diet and 4 mg/kg bw (garlic + cheese probiotic supplementation in 1:1 ratio respectively. Ingestion of AFB1-contaminated fish feed possess the adverse effects on hematological parameters like, total red blood cells numbers, relative number of lymphocytes, monocytes, neutrophils, basophils, and eosinophils in blood. Likewise AFB1 altered globulin, albumins, and total protein concentrations in serum fractions (GroupT2. Supplementation of probiotics cheese alone showed significant protective effect than garlic and in combination group (Group T5. Group 5 showed more or less similar to that of the control, in conclusion probiotics cheese and garlic showed significant combat effects in reducing hematological toxic effects of AFB1.

  8. Seasonal distribution, aggregation, and habitat selection of common carp in Clear Lake, Iowa

    Science.gov (United States)

    Penne, C.R.; Pierce, C.L.

    2008-01-01

    The common carp Cyprinus carpio is widely distributed and frequently considered a nuisance species outside its native range. Common carp are abundant in Clear Lake, Iowa, where their presence is both a symptom of degradation and an impediment to improving water quality and the sport fishery. We used radiotelemetry to quantify seasonal distribution, aggregation, and habitat selection of adult and subadult common carp in Clear Lake during 2005-2006 in an effort to guide future control strategies. Over a 22-month period, we recorded 1,951 locations of 54 adults and 60 subadults implanted with radio transmitters. Adults demonstrated a clear tendency to aggregate in an offshore area during the late fall and winter and in shallow, vegetated areas before and during spring spawning. Late-fall and winter aggregations were estimated to include a larger percentage of the tracked adults than spring aggregations. Subadults aggregated in shallow, vegetated areas during the spring and early summer. Our study, when considered in combination with previous research, suggests repeatable patterns of distribution, aggregation, and habitat selection that should facilitate common carp reduction programs in Clear Lake and similar systems. ?? Copyright by the American Fisheries Society 2008.

  9. Expression of sglt1 gene in Cyprinus carpio and preparation of its polyclonal antibody%鲤肠道sglt1基因的表达与抗体制备

    Institute of Scientific and Technical Information of China (English)

    聂国兴; 王贝; 闫潇; 侯彩霞; 张建新; 张新胜; 郑俊林; 王俊丽

    2012-01-01

    钠/葡萄糖共转运载体1( sodium/glucose cotransporter 1,Sglt1)是协助葡萄糖吸收的主要蛋白.实验首先采用RT-PCR获取sglt1基因全长,克隆至PGME-T载体进行序列及免疫原性分析,选择长度为92个氨基酸(544 ~637)的多肽作为目的片段(sglt1-P)),扩增sglt1-P,引入双酶切位点EcoR I和HindⅢ后,连接至pET-32a(+)上,构建表达载体pET-32a(+)-sglt1-P,转化至E.coli Rosetta中,获得重组基因工程菌,通过IPTG诱导表达,获得目标多肽,并以此为抗原制备Sglt1特异性抗体.SDS-PAGE电泳分析表明,目标多肽分子量约为30 ku.采用耳缘静脉结合皮下注射,免疫新西兰长耳兔,免疫总时长为38 d.制备了鲤Sglt1抗体,ELISA测得效价为1∶105;免疫组化结果表明,抗体具有较高亲和力和特异性,可以应用于鲤Sglt1的表达定位研究.该抗体的获得为鲤肠道Sglt1表达及转运活性的系统研究奠定了基础,同时,获取的Sglt1抗体亦可用于其它鱼类Sglt1转运蛋白表达定位和定量研究.%The high-affinity Na+/glucose contransporter Sgltl is one of the important members of the sodium: solute symporter family (SSF), belonging to the homologous family 5(SLC5). The Sgltl plays an important role in accumulating glucoses from intestinal or kidney epithelial cells against an adverse concentration gradient and maintaining the adjustment of metabolism. So far, few studies on Na+/glucose cotransporter have been reported in the freshwater fishes. This research would develop special antibodies of Sgltl, which could supply the foundation for the research of glucose metabolism in Cyprinus carpio intestines on molecular level. To study the molecular mechanism of glucose metabolism in freshwater fishes,the full-length cDNA of sgltl in C. Carpio with an ORF of 1 977 bp was cloned and the antigenicity of Sgltl was first predicted. 92 (544 - 637 ) amino acids with strong antigenicity and immunogenicity were selected as target section of sgltl in C

  10. Účinek pesticidu s účinnou látkou diazinon na kapra obecného (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Žlábek, V.; Svoboda, M.; Lusková, Věra

    České Budějovice : Jihočeská univerzita, 2001 - (Máchová, J.; Svobodová, Z.; Randák, T.), s. 129-138 [Toxicita a biodegradabilita odpadů a látek významných ve vodním prostředí /10./. České Budějovice (CZ), 20.08.2001-22.08.2001] Institutional research plan: CEZ:AV0Z6093917 Keywords : common carp * pesticide Subject RIV: GL - Fishing

  11. 不同蛋白水平的虾青素饲料对锦鲤体色、生长及免疫的影响%Effects of dietary protein levels on body pigmentation, growth and immunology of ornamental carp( Cyprinus carpio L. )

    Institute of Scientific and Technical Information of China (English)

    崔培; 姜志强; 王雪; 张源真; 高小强; 田青杰

    2012-01-01

    A feeding trial was conducted to investigate the effects of dietary protein levels on body pigmentation, growth and immunology of ornamental carpCyprinus carpio L. ) (initial mean body weight: 5.85 ±0.19 g). The diet contained 130 mg/kg astaxanthins: Fish were fed with different protein levels (25.49% ,30.84% ,35.04% ,40.68% ,45.88% ) and fed to apparent satiety to these groups of 20 fish for 60 days. The results showed that: The value of a * was highest in the protein level of 35.04% , no significant differences compared with protein level 40.68 % ( P 〈 0.05 ) , but obviously higher than other experimental groups (P 〉 0.05 ). The concentration of carotenoid in the skin of fish had significant differences from other experimental groups (P 〈 0.05 )at the same level while the highest value in the group protein level 45.88%. The highest body weight gain and feed conversion ratio occurred in the protein level 35.04%. There were no significant effects on LZM, CAT and LDH ( P 〉 0.05 ). While the activity of SOD decreased obviously at the protein level of 45. 88% ( P 〈 0. 05 ). The results of this study also illustrated that the utilization of astaxanthin of ornamental carp( Cyprinus carpio L)did not improve constantly with the increase of protein level in diet.%以初始体重为(5.85±0.19)g的红白锦鲤幼鱼为实验对象,研究虾青素有效添加量为130 mg/kg时,饲料蛋白水平(25.49%、30.84%、35.04%、40.68%、45.88%)对锦鲤体色、生长及免疫的影响。每组设3个平行,每个平行饲养20尾鱼,表观饱食投喂60 d后,实验结果显示:饲料蛋白水平为35.04%时,实验鱼体表红质a*值最高,与蛋白水平40.68%组间无显著差异(P〉0.05),但显著高于其他实验组(P〈0.05),与此同时,皮肤中类胡萝卜素含量显著升高(P〈0.05),最大值出现在蛋白水平45.88%组。蛋白水平为35.04%时,实验鱼的增重率(RWG)、特

  12. Pelacakan Gen Aerolysin dari Aeromonas hydrophila pada Ikan Mas yang Diberi Pakan Ekstrak Bawang Putih (DETECTION OF AEROLYSIN GEN FROM AEROMONAS HYDROPHILA IN COMMON CARP FED WITH GARLIC EXTRACT

    Directory of Open Access Journals (Sweden)

    Iesje Lukistyowati

    2014-04-01

    Full Text Available Aeromonas hydrophila is a gram negative and opportunistic bacteria, which could cause fish mortalityin a short time from 80%-100%. One virulent factor of A. hydrophila on common carp (Cyprinus carpio Lthat could cause fish mortality is aerolysin. This research used a synthetic primers of oligonukleotide todetect aerolysin, a specific genomes of A. hydrophila on common carp (Cyprinus carpio L. The commoncarps have been feed a woof that contain garlic extract during 30 days before they challenged with A.hydrophila. Polymerase Chain Reaction (PCR was used to detect an aerolysin gen from A. hydrophila. Theelectrophoresis result showed aerolysin gene of Aeromonas hydrophila from Veterinary Faculty of GadjahMada University (FKH-UGM isolate was amplified with 462 bp of molecule weight. While the aerolysingen was detected in the fish kidney with 900 bp of molecule weight. Further, DNA sequence analysis of thePCR product of A. hydrophila from FKH – UGM isolate showed homolog with isolate A. hydrophila subsphydrophila ATCC 7966 complete genome with score 55.4 (71%.

  13. Effect of relative volume on radio transmitter expulsion in subadult common carp

    Science.gov (United States)

    Penne, C.R.; Ahrens, N.L.; Summerfelt, R.C.; Pierce, C.L.

    2007-01-01

    Expulsion of surgically implanted radio transmitters is a problem in some fish telemetry studies. We conducted a 109-d experiment to test the hypothesis that variation in relative volume of transmitters surgically implanted in subadult common carp Cyprinus carpio would affect transmitter expulsion. We also necropsied fish at the end of the experiment to evaluate histological evidence for the mechanism of expulsion. Survival rate was high during our experiment; all control fish and 88% of the fish subjected to the implantation surgery survived. Expulsion rate was low; of the 23 fish that received transmitters and survived the experiment, only two (9%) expelled the transmitters. One of these expulsions occurred through a rupture of the incision and the other occurred via the intestine. Retained transmitters were all encapsulated by tissue, and most exhibited multiple adhesions to the intestine, gonads, and body wall. Adhesions were more numerous in fish that received larger transmitters. ?? Copyright by the American Fisheries Society 2007.

  14. Cloning of the Koi Herpesvirus Genome as an Infectious Bacterial Artificial Chromosome Demonstrates That Disruption of the Thymidine Kinase Locus Induces Partial Attenuation in Cyprinus carpio koi▿

    Science.gov (United States)

    Costes, B.; Fournier, G.; Michel, B.; Delforge, C.; Raj, V. Stalin; Dewals, B.; Gillet, L.; Drion, P.; Body, A.; Schynts, F.; Lieffrig, F.; Vanderplasschen, A.

    2008-01-01

    Koi herpesvirus (KHV) is the causative agent of a lethal disease in koi and common carp. In the present study, we describe the cloning of the KHV genome as a stable and infectious bacterial artificial chromosome (BAC) clone that can be used to produce KHV recombinant strains. This goal was achieved by the insertion of a loxP-flanked BAC cassette into the thymidine kinase (TK) locus. This insertion led to a BAC plasmid that was stably maintained in bacteria and was able to regenerate virions when permissive cells were transfected with the plasmid. Reconstituted virions free of the BAC cassette but carrying a disrupted TK locus (the FL BAC-excised strain) were produced by the transfection of Cre recombinase-expressing cells with the BAC. Similarly, virions with a wild-type revertant TK sequence (the FL BAC revertant strain) were produced by the cotransfection of cells with the BAC and a DNA fragment encoding the wild-type TK sequence. Reconstituted recombinant viruses were compared to the wild-type parental virus in vitro and in vivo. The FL BAC revertant strain and the FL BAC-excised strain replicated comparably to the parental FL strain. The FL BAC revertant strain induced KHV infection in koi carp that was indistinguishable from that induced by the parental strain, while the FL BAC-excised strain exhibited a partially attenuated phenotype. Finally, the usefulness of the KHV BAC for recombination studies was demonstrated by the production of an ORF16-deleted strain by using prokaryotic recombination technology. The availability of the KHV BAC is an important advance that will allow the study of viral genes involved in KHV pathogenesis, as well as the production of attenuated recombinant candidate vaccines. PMID:18337580

  15. Comparative study of seasonal variation in bacterial flora concomitant with farm raised fingerlings of Cyprinus carpio at tarai region of Uttarakhand.

    Science.gov (United States)

    Bisht, A; Singh, U P; Pandey, N N

    2014-03-01

    The bacterial infection is an important economic and limiting factor in intensive fish production. The present study focuses on investigation of the bacterial population associated with farmed common carp fingerlings, its environment and limnological quality of pond, during winter and summer season. It was found that the bacterial count in the pond sediment (6.40 cfu x 10(4)) was about 10 times higher in comparison of pond water (6.93 cfu x 10(3)). Further, the intestinal bacterial count was about 100 times higher (6.67 cfu x 10(5)) during winter and 1000 times higher (2.33 cfu x 10(6)) during summer season in comparison to the surfacial skin of fish during winter and summer (3.39 and 8.87 cfu x 10(3)), respectively. The isolated bacteria were both Gram negative and Gram positive, mostly aerobic rods. Furthermore, the temperature showed a significant relation with the bacterial counts of pond water. In the summer season, higher bacterial counts (8.72 cfu x 10(3)) were recorded as compared to winter (5.13 cfu x 10(3)). The dominant bacteria isolated from the sample of pond water, pond sediment and fish were identified as Aeromonas hydrophila, Pseudomonas fluorescens, Pseudomonas sp., Flavobacter sp., Bacillus sp., Micrococcus sp., Corynebacterium sp. Moreover, the bacterial density was dependent on C:N values, and the optimum range of C: N ratio was found between 16-23, for the carp culture ponds. Among the isolated bacterial flora, the presence of strains which were well known for their probiotic properties suggested an autochthonous source for use in aquaculture. Further, analysis of various physico-chemical parameters of pond water revealed that they were within the suitable range for the freshwater fish culture throughout farming phase. PMID:24665763

  16. Effect of Cadmium Chloride on Metallothionein Levels in Carp

    Directory of Open Access Journals (Sweden)

    Jana Kovarova

    2009-06-01

    Full Text Available Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. It is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. In this work the effects of cadmium chloride (2.5, 5, 7.5, 10 and 12.5 mg/L on common carp (Cyprinus carpio was investigated. We determined cadmium content in tissue of muscle, liver and kidney by atomic absorption spectrometry with electrothermal atomization and content of metallothionein (MT in the same tissues by the Brdicka reaction. Electrochemical methods can be considered as suitable and sensitive tools for MT determination in carp tissues. Results of our study showed a gradually enhancing of cadmium content in muscle with time and dose of cadmium chloride in water. MT levels in liver reached both high levels (above 130 ng/g in fish exposed to 2.5, 5 and 7.5 mg/L and low level (to 50 ng/g in fish exposed to 10 and 12.5 mg/L of cadmium chloride. This finding confirms that the synthesis of metallothioneins and binding capacity of these proteins is restricted.

  17. Effects of different oxygen saturation on activity of complex biomass and aqueous crude extract of cyanobacteria during embryonal development in carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Palíková, M.; Krejčí, R.; Hilscherová, Klára; Buryšková, B.; Babica, Pavel; Navrátil, S.; Kopp, R.; Bláha, Luděk

    2007-01-01

    Roč. 76, č. 2 (2007), s. 291-299. ISSN 0001-7213 R&D Projects: GA AV ČR KJB6005411 Institutional research plan: CEZ:AV0Z60050516 Keywords : ammonia * cyanobacteria * embryonal test Subject RIV: EF - Botanics Impact factor: 0.687, year: 2007

  18. [Phosphorylation of Cl-, HCO3--stimulated Mg2+-ATPase of plasma membranes of carp (Cyprinus carpio L.) brain sensitive to GABA(A)-ergic ligands].

    Science.gov (United States)

    Menzikov, S A; Menzikova, O V

    2006-01-01

    Phosphorylation of the sensitive to GABA(A)-ergic ligands Cl-, HCO3--stimulated Mg2+-ATPase of the plasma membranes from fish brain by [gamma-32P]ATP was investigated in the presence of Mg2+. It was established, that formation of the phosphoprotein at 0-1 degrees C is dependent on time incubation and concentration of Mg2+ in the incubation medium. Hydroxylamine (50 mM) and pH (10) completely inhibited formation of phosphorylated intermediate. Ions of Cl- (10 mM)+HCO3- (2 mM) and also GABA (1-100 microM) dephosphorylated the enzyme. The dephosphorylating effect of GABA on the membrane samples did not appear in the presence of bicuculline. o-Vanadate (10 microM) eliminates the dephosphorylating effect of anions and GABA on the phosphoprotein. It was established by SDS-PAAG electrophoresis and autoradiographia that investigated phosphorylation and GABA(A)-induced dephosphorylation is performed by the protein with molecular weight aproximately 56 kDa. Such molecular weight has a subunit which forms oligomer composition of the sensitive to GABA(A)-ergic ligands Cl-, HCO3--ATPase from fish brain. The obtained data demonstrated that Cl, HCO3- ATPase from fish brain can be directly phosphorylated by [gamma-32P]ATP in the presence of Mg2+ and forms the phosphorylation intermediate. PMID:17147268

  19. The effect of the fluoroquinolone norfloxacin on somatic indices and oxidative stress parameters in early stages of common carp (Cyprinus carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Charvátová, N.; Želinská, G.; Dobšíková, R.; Stancová, V.; Živná, D.; Plhalová, L.; Blahová, J.; Sehonová, P.; Maršálek, P.; Bartošková, M.; Prokeš, Miroslav; Piskořová, I.; Svobodová, Z.

    2015-01-01

    Roč. 36, Supplement 1 (2015), s. 79-87. ISSN 0172-780X Institutional support: RVO:68081766 Keywords : antibiotics * fish * morphometry * antioxidant enzymes * lipid peroxidation Subject RIV: EG - Zoology Impact factor: 0.799, year: 2014

  20. Enzyme treatment for elimination of egg stickness in tench (Tinca Tinca L.), European catfish (Silurus Glanis L.) and common carp (Cyprinus Carpio L.)

    Czech Academy of Sciences Publication Activity Database

    Linhart, Otomar; Rodina, Marek; Gela, David; Flajšhans, Martin; Kocour, Martin

    2004-01-01

    Roč. 28, - (2004), s. 507-508. ISSN 0920-1742 R&D Projects: GA MŠk ME 403; GA MŠk ME 420; GA AV ČR IBS5045314 Institutional research plan: CEZ:AV0Z5045916 Keywords : aquaculture * egg * enzyme Subject RIV: ED - Physiology Impact factor: 0.399, year: 2004

  1. Effects of Pro-Tex on zebrafish (Danio rerio) larvae, adult common carp (Cyprinus carpio) and adult yellowtail kingfish (Seriola lalandi)

    NARCIS (Netherlands)

    Boerrigter, J.G.J.; Vis, van de J.W.; Bos, van den R.; Abbink, W.; Spanings, T.; Zethof, J.; Louzao Martinez, L.; Andel, van W.F.M.; Lopez-Luna, J.; Flik, G.

    2014-01-01

    Aquaculture practices bring several stressful events to fish. Stressors not only activate the hypothalamus–pituitary–interrenal-axis, but also evoke cellular stress responses. Up-regulation of heat shock proteins (HSPs) is among the best studied mechanisms of the cellular stress response. An extract

  2. Therapeutic Effect of Phyllanthus emblica on Disease Induced Common Carp Cyprinus carpio by Aeromonas hydrophila

    OpenAIRE

    A. Valsa Judit Anto; Balasubramanian, V.

    2015-01-01

    The fresh water fish are the important source of animal protein in human population. Fish can adapt themselves to adverse environmental conditions by changing their physiological activities. Immunostimulants like amla (Phyllanthus emblica) has the additional effects such as enhancement of growth and increase in the survival rate of the fish under stress. Hematological studies on fish have assumed greater significance due to increasing emphasis on pisciculture and greater awareness on natural ...

  3. Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media

    Czech Academy of Sciences Publication Activity Database

    Opršal, J.; Bláha, L.; Pouzar, M.; Knotek, P.; Vlček, Milan; Hrdá, K.

    2015-01-01

    Roč. 22, č. 23 (2015), s. 19124-19132. ISSN 0944-1344 Institutional support: RVO:61389013 Keywords : nanosilver * agglomeration * fish embryo Subject RIV: DJ - Water Pollution ; Quality Impact factor: 2.828, year: 2014

  4. Molecular cloning, characterization and expression analysis of TLR9, MyD88 and TRAF6 genes in common carp (Cyprinus carpio)

    Science.gov (United States)

    Induction of innate immune pathways is critical for early host defense but there is limited understanding of how teleost fish recognize pathogen molecules and activate these pathways. In mammals, cells of the innate immune system detect pathogenic molecular structures using pattern recognition rece...

  5. Biochemical parameters of blood plasma and content of microcystins in tissues of common carp (Cyprinus carpio L.) from hypertrophic pond with cyanobacterial water bloom

    Czech Academy of Sciences Publication Activity Database

    Kopp, Radovan; Mareš, J.; Palíková, M.; Navrátil, S.; Kubíček, Z.; Ziková, A.; Hlávková, J.; Bláha, Luděk

    2009-01-01

    Roč. 40, č. 15 (2009), s. 1683-1693. ISSN 1355-557X Institutional research plan: CEZ:AV0Z60050516 Keywords : microcystin * aquaculture * biochemistry Subject RIV: EF - Botanics Impact factor: 1.099, year: 2009

  6. Consequent effects of the great cormorant (Phalacrocorax carbo sinensis) predation on parasite infection and body condition of common carp (Cyprinus carpio)

    Czech Academy of Sciences Publication Activity Database

    Ondračková, Markéta; Valová, Zdenka; Kortan, J.; Vojtek, L.; Adámek, Z.

    2012-01-01

    Roč. 110, č. 4 (2012), s. 1487-1493. ISSN 0932-0113 R&D Projects: GA MŠk LC522 Institutional support: RVO:68081766 Keywords : Ichthyophthirius multifiliis * Channel catfish * Crowding stress * Arctic charr * Fish * Susceptibility * Monogeneans Subject RIV: EG - Zoology Impact factor: 2.852, year: 2012

  7. Fish-friendly prophylaxis/disinfection in aquaculture: Low concentration of peracetic acid is stress-free to the carp (Cyprinus carpio) after repeated applications

    Science.gov (United States)

    Application of peracetic acid (PAA) at low concentrations has been proved to be a broad functional and eco-friendly prophylaxis/disinfection method against various fish pathogens. Therefore, regular applications of low concentration PAA is sufficient to control (potential) pathogens in recirculatin...

  8. Effects of carbon nanomaterials fullerene C{sub 60} and fullerol C{sub 60}(OH){sub 18-22} on gills of fish Cyprinus carpio (Cyprinidae) exposed to ultraviolet radiation

    Energy Technology Data Exchange (ETDEWEB)

    Socoowski Britto, Roberta; Longaray Garcia, Marcia; Martins da Rocha, Alessandra [Universidade Federal do Rio Grande - FURG, Instituto de Ciencias Biologicas, Campus Carreiros, Av. Italia km 8 s/n, Rio Grande, RS (Brazil); Programa de Pos Graduacao em Fisiologia Animal Comparada - Fisiologia Animal Comparada, FURG (Brazil); Artigas Flores, Juliana [Universidade Federal do Rio Grande - FURG, Instituto de Ciencias Biologicas, Campus Carreiros, Av. Italia km 8 s/n, Rio Grande, RS (Brazil); Pinheiro, Mauricio V. Brant [Departamento de Fisica, ICEx, Universidade Federal de Minas Gerais, MG (Brazil); Monserrat, Jose Maria [Universidade Federal do Rio Grande - FURG, Instituto de Ciencias Biologicas, Campus Carreiros, Av. Italia km 8 s/n, Rio Grande, RS (Brazil); Programa de Pos Graduacao em Fisiologia Animal Comparada - Fisiologia Animal Comparada, FURG (Brazil); Ribas Ferreira, Josencler L., E-mail: josenclerf@gmail.com [Universidade Federal do Rio Grande - FURG, Instituto de Ciencias Biologicas, Campus Carreiros, Av. Italia km 8 s/n, Rio Grande, RS (Brazil); Programa de Pos Graduacao em Fisiologia Animal Comparada - Fisiologia Animal Comparada, FURG (Brazil)

    2012-06-15

    In consequence of their growing use and demand, the inevitable environmental presence of nanomaterials (NMs) has raised concerns about their potential deleterious effects to aquatic environments. The carbon NM fullerene (C{sub 60}), which forms colloidal aggregates in water, and its water-soluble derivative fullerol (C{sub 60}(OH){sub 18-22}), which possesses antioxidant properties, are known to be photo-excited by ultraviolet (UV) or visible light. To investigate their potential hazards to aquatic organisms upon exposure to UV sunlight, this study analyzed (a) the in vitro behavior of fullerene and fullerol against peroxyl radicals (ROO{center_dot}) under UV-A radiation and (b) the effects of these photo-excited NMs on oxidative stress parameters in functional gills extracted from the fish Cyprinus carpio (Cyprinidae). The variables measured were the total antioxidant capacity, lipid peroxidation (TBARS), the activities of the antioxidant enzymes glutathione reductase (GR) and glutamate cysteine ligase (GCL), and the levels of the non-enzymatic antioxidant glutathione (GSH). The obtained results revealed the following: (1) both NMs behaved in vitro as antioxidants against ROO{center_dot} in the dark and as pro-oxidants in presence of UV-A, the latter effect being reversed by the addition of sodium azide, which is a singlet oxygen ({sup 1}O{sub 2}) quencher; (2) fullerene induced toxicity with or without UV-A incidence, with a significant (p < 0.05) increase in lipid peroxidation (with greater damage under illumination), a decrease in GCL activity, and the depletion of GSH stocks (under illumination), all of which were attributed to {sup 1}O{sub 2} generation; and (3) fullerol also decreased GCL activity and GSH formation (p < 0.05) but without lipid damage. The overall results show that fullerene can be toxic with or without light incidence, whereas UV radiation seems to play a key role in the environmental toxicity of carbon NMs through {sup 1}O{sub 2} formation.

  9. Identification and characterization of alpha-I-proteinase inhibitor from common carp sarcoplasmic proteins.

    Science.gov (United States)

    Siriangkanakun, Siriphon; Li-Chan, Eunice C Y; Yongsawadigul, Jirawat

    2016-02-01

    Purification of proteinase inhibitor from common carp (Cyprinus carpio) sarcoplasmic proteins resulted in 2.8% yield with purification fold of 111. Two inhibitors, namely inhibitor I and II, exhibited molecular mass of 47 and 52 kDa, respectively, based on non-reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both inhibitors I and II were identified to be alpha-1-proteinase inhibitor (α1-PI) based on LC-MS/MS. They were glycoproteins and molecular mass after peptide-N-glycosidase F treatment was 38 and 45 kDa, respectively. The N-glycosylation sites of both inhibitors were determined to be at N214 and N226. The inhibitors specifically inhibited trypsin. The common carp α1-PI showed high thermal stability with denaturation temperatures of 65.43 and 73.31 °C, which were slightly less than those of ovomucoid. High stability toward NaCl was also evident up to 3M. The common carp α1-PI effectively reduced autolytic degradation of bigeye snapper surimi at the concentration as low as 0.025%. PMID:26304452

  10. The relationship between the distribution of common carp and their environmental DNA in a small lake.

    Directory of Open Access Journals (Sweden)

    Jessica J Eichmiller

    Full Text Available Although environmental DNA (eDNA has been used to infer the presence of rare aquatic species, many facets of this technique remain unresolved. In particular, the relationship between eDNA and fish distribution is not known. We examined the relationship between the distribution of fish and their eDNA (detection rate and concentration in a lake. A quantitative PCR (qPCR assay for a region within the cytochrome b gene of the common carp (Cyprinus carpio or 'carp', an ubiquitous invasive fish, was developed and used to measure eDNA in Lake Staring (MN, USA, in which both the density of carp and their distribution have been closely monitored for several years. Surface water, sub-surface water, and sediment were sampled from 22 locations in the lake, including areas frequently used by carp. In water, areas of high carp use had a higher rate of detection and concentration of eDNA, but there was no effect of fish use on sediment eDNA. The detection rate and concentration of eDNA in surface and sub-surface water were not significantly different (p≥0.5, indicating that eDNA did not accumulate in surface water. The detection rate followed the trend: high-use water > low-use water > sediment. The concentration of eDNA in sediment samples that were above the limit of detection were several orders of magnitude greater than water on a per mass basis, but a poor limit of detection led to low detection rates. The patchy distribution of eDNA in the water of our study lake suggests that the mechanisms that remove eDNA from the water column, such as decay and sedimentation, are rapid. Taken together, these results indicate that effective eDNA sampling methods should be informed by fish distribution, as eDNA concentration was shown to vary dramatically between samples taken less than 100 m apart.

  11. Comparative genomics in cyprinids: common carp ESTs help the annotation of the zebrafish genome

    Directory of Open Access Journals (Sweden)

    Srinivasan Hamsa

    2006-12-01

    Full Text Available Abstract Background Automatic annotation of sequenced eukaryotic genomes integrates a combination of methodologies such as ab-initio methods and alignment of homologous genes and/or proteins. For example, annotation of the zebrafish genome within Ensembl relies heavily on available cDNA and protein sequences from two distantly related fish species and other vertebrates that have diverged several hundred million years ago. The scarcity of genomic information from other cyprinids provides the impetus to leverage EST collections to understand gene structures in this diverse teleost group. Results We have generated 6,050 ESTs from the differentiating testis of common carp (Cyprinus carpio and clustered them with 9,303 non-gonadal ESTs from CarpBase as well as 1,317 ESTs and 652 common carp mRNAs from GenBank. Over 28% of the resulting 8,663 unique transcripts are exclusively testis-derived ESTs. Moreover, 974 of these transcripts did not match any sequence in the zebrafish or fathead minnow EST collection. A total of 1,843 unique common carp sequences could be stringently mapped to the zebrafish genome (version 5, of which 1,752 matched coding sequences of zebrafish genes with or without potential splice variants. We show that 91 common carp transcripts map to intergenic and intronic regions on the zebrafish genome assembly and regions annotated with non-teleost sequences. Interestingly, an additional 42 common carp transcripts indicate the potential presence of new splicing variants not found in zebrafish databases so far. The fact that common carp transcripts help the identification or confirmation of these coding regions in zebrafish exemplifies the usefulness of sequences from closely related species for the annotation of model genomes. We also demonstrate that 5' UTR sequences of common carp and zebrafish orthologs share a significant level of similarity based on preservation of motif arrangements for as many as 10 ab-initio motifs. Conclusion

  12. Effect of feeding carp with fat-supplemented pelleted diets on histological appearance of the intestine and hepatopancreas

    Directory of Open Access Journals (Sweden)

    Piotr Epler

    2009-06-01

    Full Text Available Sixty two-year-old carps, Cyprinus carpio L. were kept in a closed water circulation system for five months. Fish were assigned to five groups (12 fish per group and fed ad libitum on Aller Classic pelleted feed for carp: standard or standard pelleted mixture supplemented with 6% oils. The control group (IK received standard pellets, group IIS+Rz – sunflower oil + rapeseed oil (50% : 50%; group IIIS+L – sunflower oil + linseed oil (80% : 20%; group IVR – fish oil; and group VSK – pork scratchings at the level of 6%. At the end of the experiment, 10 carps from each group were slaughtered and their body length and weight were measured to calculate mean body weight gains and mean individual gains. Fragments of intestine and hepatopancreas were histologically and morphometrically analyzed. The mean weight gains of fish at the end of the experiment, which were the highest for diet IIIS+L, and the lowest for VSK and IK diets, were fully confirmed by histological analysis, which showed that the lowest gains in biomass and mean individual weight were due to digestive disturbances associated mainly with lipid metabolism. These disturbances did not occur in groups IIS+Rz, IIIS+L and IVR.

  13. Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6

    Indian Academy of Sciences (India)

    Jing Liu; Yong-Hua Sun; Na Wang; Ya-Ping Wang; Zuo-Yan Zhu

    2006-12-01

    Some members of hairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes, the her6 gene, a member of the HES family, is the homologue of hes1 in mammals and chicken. In this study, the her6 gene and its full-length cDNA from the common carp (Cyprinus carpio) were isolated and characterized. The genomic sequence of common carp her6 is approximately 1.7 kb, with four exons and three introns, and the full-length cDNA of 1314 bp encodes a putative polypeptide of 271 amino acids. To analyse the promoter sequence of common carp her6, sequences of various lengths upstream from the transcription initiation site of her6 were fused to enhanced green fluorescent protein gene (eGFP) and introduced into zebrafish embryos by microinjection to generate transgenic embryos. Our results show that the upstream sequence of 500 bp can direct highly efficient and tissue-specific expression of eGFP in zebrafish embryos, whereas a fragment of 200 bp containing the TATA box and a partial suppressor of hairless paired site sequence (SPS) is not sufficient to drive eGFP expression in zebrafish embryos.

  14. Influence of potassium permanganate, cobalt chloride, and dietary supplement of vitamin B complex on the histopathological changes in gill epithelium of common carp exposed to cadmium

    Energy Technology Data Exchange (ETDEWEB)

    Das, B.K.; Kaviraj, A. [Univ. of Kalyani, West Bengal (India)

    1994-10-01

    Fry of common carp (Cyprinus carpio) were chronically exposed to 2.5 mg Cd/L alone and in combination with 1.0 mg KMnO{sub 4}/L or 2.0 mg CoCl{sub 2}/L or a dietary supplement of vitamin B complex at the rate of 26.5 mg/100 g food. Cadmium induced edema of primary and secondary gill lamellae, nuclear swelling, and necrosis and hypertrophy of epithelial cells of the secondary gill lamellae. Similar or more severe lamellar damages were observed with exposure to cadmium together with potassium permanganate and to cadmium together with cobalt chloride. Potassium permanganate alone was also found to produce severe edema of the gill lamellae. A dietary supplement of vitamin B complex reduced the cadmium-induced gill damages and resulted in a normal gill in exposed fish. 24 refs., 4 figs., 1 tab.

  15. Acute toxicity and tissue injury studies of ivermectin for Songpu mir-ror carp (Cyprinus specularis songpu)%伊维菌素对松浦镜鲤的急性毒性与组织损伤

    Institute of Scientific and Technical Information of China (English)

    韩冰; 王荻; 卢彤岩

    2015-01-01

    分别以1.30 mg/kg、1.69 mg/kg、2.19 mg/kg、2.86 mg/kg、3.71 mg/kg、4.83 mg/kg、6.27 mg/kg、8.16 mg/kg剂量的伊维菌素(ivermectin)对松浦镜鲤(Cyprinus specularis songpu)进行口灌,并设置空白对照和溶剂对照,给药后连续观察96 h,记录各组鱼的行为和死亡情况。用 SPPS 软件计算得出松浦镜鲤口灌伊维菌素的96 h LD50为4.04 mg/kg,95%可信区间为3.42~4.80 mg/kg。对给药组和对照组鱼进行血细胞分析,发现随着给药剂量的增加,红细胞数无明显变化,血红蛋白浓度呈上升趋势,而白细胞数呈下降趋势。通过组织切片观察,发现伊维菌素给药剂量为4.83 mg/kg 时松浦镜鲤体内组织开始出现明显的病理变化。大剂量口灌伊维菌素后,松浦镜鲤肠绒毛出现坏死、脱落现象,肝细胞萎缩、肝血窦变大,肾小管上皮细胞变性、坏死,心肌纤维萎缩、变性。本研究通过探讨伊维菌素对松浦镜鲤的毒性效应,旨在为松浦镜鲤养殖过程中科学合理的使用伊维菌素提供基础数据和科学依据。%In order to evaluate the toxic effects of ivermectin for Songpu mirror carp, the acute toxicity and tissue injury of ivermectin for Songpu mirror carp was studied. Ivermectin was given by oral administration to Songpu mirror carp at the dosage of 1.30 mg/kg, 1.69 mg/kg, 2.19 mg/kg, 2.86 mg/kg, 3.71 mg/kg, 4.83 mg/kg, 6.27 mg/kg, 8.16 mg/kg re-spectively. Blank control and solvent control were sited also. After given ivermectin, the fish were investigated in 96 h;the dead fish were sacrificed at once. Through calculating by SPSS, the 96h LD50 and 95% confidence of LD50 of iver-mectin for Songpu mirror carp were 4.04 mg/kg and 3.42~4.80 mg/kg, respectively. The results show that with the in-crease of the dose, the number of red blood cells had no obvious change, the hemoglobin concentration had rised and the white blood cell count had decreased, according to analysis blood cell of the administration

  16. Activities of two lipases and effects of m-cresol on them during early development of Cyprinus carpio var.color%瓯江彩鲤早期发育中两种脂酶活性及间甲酚对其活性的影响

    Institute of Scientific and Technical Information of China (English)

    朱俊华; 姚俊杰; 冯亚楠; 邹芳芳; 莫巨鼎

    2014-01-01

    Activities of lipoprotein lipase (LPL) and hepatic lipase (HL) were determined during early development of Cyprinus carpio var.color, and the effects of m-cresol on them were studied through a biochemical and static toxicological methods.The results showed that lipases were present in the mature eggs and fertilized eggs , and activities of lipase was the highest in the gastrula stage; after being exposed to the m-cresol at the concentration of 7 mg/L, 13 mg/L and 19 mg/L at the temperature (20 ±2) ℃, activity of lipase first rose and then declined with the increase of m -cresol concentration , HL activity of 19 mg/L concentration group in embryonic stage was significant ly different from other groups ( P<0.05 ).Compared to control group , LPL activity of 7 mg/L concentration group in embryonic and larval stage showed significant differences ( P<0.05 ).%采用生化和静态急性毒性实验方法,研究了瓯江彩鲤( Cyprinus carpio var.color)早期发育过程中脂蛋白脂酶和肝脂酶活性变化及间甲酚对它们活性的影响。结果显示,脂酶在瓯江彩鲤成熟卵和受精卵中均有活性,脂蛋白脂酶和肝脂酶的活性在胚胎发育过程中总体上呈上升趋势,原肠胚期酶活性最高;在水温(20±2)℃条件下,经7 mg/L、13 mg/L和19 mg/L的间甲酚暴露,脂酶活性随着间甲酚的浓度升高先升高后下降,19 mg/L浓度组中肝脂酶活性在胚胎期与其他组均有显著差异( P<0.05);与对照组相比,7 mg/L浓度组脂蛋白脂酶活性在胚胎和仔鱼期均有显著差异( P<0.05)。

  17. Matrix Metalloproteinase 2 (MMP-2) Plays a Critical Role in the Softening of Common Carp Muscle during Chilled Storage by Degradation of Type I and V Collagens.

    Science.gov (United States)

    Xu, Chao; Wang, Cheng; Cai, Qiu-Feng; Zhang, Qian; Weng, Ling; Liu, Guang-Ming; Su, Wen-Jin; Cao, Min-Jie

    2015-12-30

    Matrix metalloproteinases (MMPs) are proposed to play important roles in the degradation of collagens, thus causing the post-mortem softening of fish muscle, although the specific mechanism remains largely unresolved. Previously, we reported the existence of gelatinase-like proteinases in common carp (Cyprinus carpio) muscle. The primary structures of these proteinases, however, have never been investigated. In the present study, two MMPs with molecular masses of 66 and 65 kDa were purified to homogeneity from common carp muscle by ammonium sulfate fractionation and a series of column chromatographies. Matrix-assisted laser desorption/ionization-tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS/MS) analysis indicated that they are completely identical to MMP-2 from common carp. During chilled storage of common carp at 4 °C, the enzymatic activity of MMP-2 increased to 212% in 12 h while the texture profile increased over the first 2 h and gradually decreased. On the other hand, type V collagen was purified to homogeneity and a specific polyclonal antibody against this protein was prepared. Both type I and V collagens were effectively hydrolyzed by MMP-2 at 30 °C and even at 4 °C. Furthermore, injection of metalloproteinase proteinase inhibitor EDTA into the blood vessel of live common carp suppressed post-mortem tenderization significantly. All of these results confirmed that MMP-2 is a major proteinase responsible for the degradation of collagens, resulting in the softening of fish muscle during chilled storage. PMID:26653826

  18. Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (Carassius auratus gibelio bloch) as revealed by RAPD assays.

    Science.gov (United States)

    Zhou, L; Wang, Y; Gui, J F

    2000-11-01

    Sex evolution has been a debating focus in evolutionary genetics. In lower vertebrates of reptiles, amphibians, and fish, a species or a bioform reproduces either sexually or asexually but never both. A few species were found to consist of all females in fish. These all-female species can propagate by asexual reproduction modes, such as gynogenesis and hybridogenesis. However, the coexistence of sexuality and asexuality in a single species was recently noted only in a cyprinid fish silver crucian carp, Carassius auratus gibelio. This fish had been demonstrated to be capable of gynogenesis stimulated by sperm from other related species. Surprisingly, natural populations of this fish consist of a minor but significant portion (approx. 20%) of males. As different clones with specific phenotypic and genetic characteristics have been found, and RAPD markers specific to each clone have recently been identified, this fish offers many advantages for analyzing whether or not genetic recombination occurs between different clones. In this study, artificial propagation was performed in clone F and clone D. Ovulated eggs from clone F were divided into two parts and respectively inseminated with sperm from a clone D male and from a red common carp (Cyprinus carpio) male. The control clone D individuals were selected from gynogenetic offspring of clone D activated by sperm of red common carp. The phenotype and sex ratio in the experimental groups were also observed. Using RAPD molecular markers, which allow for reliable discrimination and genetic analysis of different clones, we have revealed direct molecular evidence for gonochoristic reproduction in the gynogenetic silver crucian carp and confirmed a previous hypothesis that the silver crucian carp might reproduce both gynogenetically and gonochoristically. Therefore, we conclude that the silver crucian carp possesses two reproductive modes, i.e., gynogenetic and gonochoristic reproduction. The response mechanism of two

  19. Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran

    Directory of Open Access Journals (Sweden)

    Jaqueline Ineu Golombieski

    2008-01-01

    Full Text Available Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758, grass carp (Ctenopharyngodon idella, Valenciennes, 1844, and bighead carp (Aristichthys nobilis, Richardson, 1845 were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50 of diafuran and the acetylcholinesterase (AChE activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term: common carp: control, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1; grass carp: control, 1.0, 2.0, 3.0 and 3.5 mg L-1 and, bighead carp: control, 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 mg L-1, as well as the determination of AChE at concentrations near LC50 for these species. LC50 values (nominal concentrations were 1.81 mg L-1 for the common carp, 2.71 mg L-1 for the grass carp and, 2.37 mg L-1 for the bighead carp. All carps exposed to diafuran were lethargic (lower concentrations or immobile. Diafuran inhibited the acetylcholinesterase activity in brain (~38% and muscle (~50% of all species. Muscle of bighead carp under control treatment showed higher specific AChE activity than brain (14.44 against 5.94 µmol min-1 g protein-1, respectively. Concentrations of diafuran used for rice cropping may affect Cyprinus carpio, Ctenopharyngodon idella and Aristichthys nobilis behaviors and the AChE activities in brain and muscle of these species may be an early biomarker of toxicity of this insecticide.Exposição a inseticidas em concentrações elevadas no ambiente podem ocasionar efeitos adversos subletais em organismos aquáticos. Alevinos de carpa húngara (Cyprinus carpio, Linnaeus, 1758, carpa capim (Ctenopharyngodon

  20. Characterization of growth hormone secretagogue receptor 1(GHS-R1) genes and weight gain associated SNP loci in Cyprinus carpio var.jian%建鲤生长激素促泌素受体1基因的特性及其与增重相关SNP位点的筛选

    Institute of Scientific and Technical Information of China (English)

    俞菊华; 李红霞; 李建林; 唐永凯; 董在杰

    2012-01-01

    Growth hormone secretagogue receptors (GHS-Rs) are endogenous receptors for growth hormone secretion (ghrelin) that belong to the G-protein-coupled receptor family. GHS-Rs play a role in regulating animal growth and energy homeostasis. GHS-R is a candidate quantitative trait loci related to obesity and growth in mammals. We used RT-PCR and PCR to isolate two JlGHS-RlsJlGHS-Rla and 1b. The open reading frames of jlGHS-Rls encode 360 amino acids that share 96% identity. In addition, there are two jlGHS-Rls transcription variants, an alternatively spliced 191 bp fragment from 490 nt to 680 nt in ORF with GT-AG at both ends. These transcripts led to a premature termination of translation, encoding 184 aa, and only contained three and a half transmembrane regions, which differs from the reported intron retaining variants among tilapia. The ORF of jlGHS-Rls was separated by one intron, locating between the first and second base of A260 coden. The introns of la and lb were 676 bp and 885 bp in length, respectively. We found 32 SNPs on two jlGHS-Rls in the Cyprinus carpio var.jian population using alignment sequences from different individuals. We then genotyped 9 SNPs using PCR-RFLP. We encountered each genotype in 322 individuals, but there was an obvious bias in the distribution. The la-1C386T and Ib-E1_G1S9T sites were significantly associated with juvenile and adult fish weight gain (P<0.01 and p<0.05, respectively). Individuals with CC and GG genotypes grew faster than other individuals. In addition, a further five sites were correlated with weight gain at certain stages or sex. To determine the applicability of the markers obtained in this experiment, we tested 610 individuals from seven additional families using four markers. The C386T and G159T sites remained significantly correlated with weight gain. Therefore, these two sites can be used as references for the molecular breeding of Cyprinus carpio vax.jian.%GHS-R基因在哺乳类为候选的肥胖和生长数

  1. Rhabdovirus carpio as a causative agent of disease in rainbow trout (Oncorhynchus mykiss - Walbaum

    Directory of Open Access Journals (Sweden)

    Jeremić Svetlana

    2006-01-01

    Full Text Available High mortality of 1-year old rainbow trout occurred on a fish farm in the spring season, with clinical symptoms typical for acute septicemia. Histological examination revealed inflammatory changes and necrosis of the internal organs. The causative agent was isolated on RTG-2 and EPC cell lines and identified by serum neutralisation, enzyme-linked immunosorbent assay (ELISA and immunofluorescence (IFAT as Rhabdovirus carpio. Experimental infection using the virus isolated from rainbow trout caused disease in carp fry, as well. This is the first case of disease of rainbow trout caused by Rhabdovirus carpio, and the first reported isolation of Rhabdovirus carpio from rainbow trout in Serbia. Also, this is the first successful infection of carp with Rhabdovirus carpio isolated from rainbow trout.

  2. Pathogenesis of spring viremia of carp virus in emerald shiner Notropis atherinoides Rafinesque, fathead minnow Pimephales promelas Rafinesque and white sucker Catostomus commersonii (Lacepede).

    Science.gov (United States)

    Misk, E; Garver, K; Nagy, E; Isaac, S; Tubbs, L; Huber, P; Al-Hussinee, L; Lumsden, J S

    2016-06-01

    Spring viremia of carp (SVC) is a reportable disease to the World Organization of Animal Health (OIE) as it is known to cause significant international economic impact. In Canada, the first and only isolation of SVC virus (SVCV) was in 2006, from common carp Cyprinus carpio L., at Hamilton Harbour, Lake Ontario. The susceptibility of fathead minnow Pimephales promelas Rafinesque, emerald shiner Notropis atherinoides Rafinesque and white sucker Catostomus commersonii (Lacepede) to intraperitoneal injection of the Canadian isolate (HHOcarp06) was evaluated using experimental infection, virus isolation, quantitative reverse transcription polymerase chain reaction (qRT-PCR), histopathology and immunohistochemistry (IHC). Emerald shiner and fathead minnow were most susceptible with 43 and 53% cumulative mortality, respectively, compared with koi at 33%. Quantitative RT-PCR demonstrated that koi had high viral loads throughout the experiment. At 34 days post-infection, SVCV was detected from sampled emerald shiner and white sucker in very low titre and was not detected from fathead minnow. Koi, fathead minnow and emerald shiner had gross lesions typical of SVC disease. The histopathological picture was mostly dominated by necrotic changes in kidney, spleen, liver, pancreas and intestine. IHC further confirmed SVCV infection, and staining was largely correlated with histological lesions. PMID:26411333

  3. Swan Lake National Wildlife Refuge contaminants survey results

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Bullheads (Ictalurus sp.), carp (Cyprinus carpio), a quillback (Carpiodes cyprinus), a bigmouth buffalo (Ictiobus cyprinellus), and a turtle were collected from...

  4. Perfil de ácidos graxos e composição centesimal de carpas (Cyprinus carpio) alimentadas com ração e com dejetos suínos Fatty acid profile and proximate composition of carp (Cyprinus carpio) feed artificial food and pig manure

    OpenAIRE

    Janice Izabel Druzian; Cristiane Michele Marchesi; Adilma Regina Pippa Scamparini

    2007-01-01

    Este estudo investigou o efeito da alimentação com dejetos suínos na composição centesimal e no perfil de ácidos graxos do músculo de carpas. Amostras de carpas com 4-10 meses de idade, recolhidas em Santa Catarina, foram filetadas, trituradas e acondicionadas em atmosfera de nitrogênio (-18°C). Os valores (%) de umidade, proteína e cinzas foram: 72,24; 14,85; e 2,16, respectivamente, para carpas alimentados com ração; e 83,33; 14,48; e 3,49 para carpas alimentadas com dejetos suínos. Os ...

  5. Toxicity, distribution, accumulation and cooking loss of malathion in tissues of tilapia and common carp fishes

    Directory of Open Access Journals (Sweden)

    Hassan, I. M.

    1993-12-01

    Full Text Available The acute toxicity of malathion as an organophosphorus pesticide to both of the tested fish species, i.e. Tilapia nilotica (tilapia and cyprinus carpio (Common carp was followed by estimating the LC50 at intervals from 24h up to 96h. Tilapia was much more susceptible to malathion toxicity when compared with carp and the available data proved that the LG50 of malathion to common carp fish was 5-7 times the LC50 for tilapia. Accumulation of malathion pesticide in some organs (muscles, gills, intestine and liver of both fish species was studied after application of 200, 300 and 400 ppb malathion in water for 28 days. A proportional relation was found in the two fish species between exposure time and the applied concentrations. The rate of malathion accumulation was higher in liver followed by intestine and gills whereas the lowest concentration was found in muscles. Accumulated malathion in tilapia muscles was higher than carp; while a reversible trend was observed in most other organs. Cooking of the same fish species reduced effectively malathion content in their muscles. Frying of fish in oil lead to a higher loss percent of malathion than did the other methods of cooking.

    La toxicidad aguda de malatión, pesticida organofosforado, para las dos especies de pescado analizadas, Tilapia nilotica (tilapia y Cyprinus carpio (carpa común, fue seguida por estimación de la CL50 a intervalos desde las 24h hasta las 96h. Tilapia fue mucho más susceptible a la toxicidad del malatión cuando se comparó con carpa, y los datos disponibles proporcionaron que la CL50 del malatión para carpa común fue de 5-7 veces la GL50 para tilapia. La acumulación del pesticida malatión en algunos órganos (músculos, agallas, intestino e hígado de ambas especies de pescado fue estudiada después de la aplicación de concentraciones de 200, 300 y 400 ppb de malatión en agua durante 28 días. Se encontró una relaci

  6. Hematological profile in juvenile carp reared under a recirculating system condition

    Directory of Open Access Journals (Sweden)

    Angelica Docan

    2011-12-01

    Full Text Available The object of this paper is to investigate the influence the density of population and fish sizehas on the physiological condition of Cyprinus carpio in the light of the hematological coefficient and theerythrocyte constants. The physiology of the blood is an important parameter in order to define thegeneral condition of the organism for all teleostean fish. The investigations of the metabolic profile of theblood aimed at determining the hematologic answer of the carp under the conditions of its exposure tothe controlled modification of the following technological factors: size class, stocking density. The lowvalue of the hematocrit (27% in both experiments suggests a condition of anemia or the dehydration ofthe organism. The values registered by the hemoglobin were different, according to the size of the fish,varying between 5.22 and 5.34 g/dL blood for fish with size 65-66 g/fish, respectively 6.7-7.02 g/dLblood for fish with size 150-152 g, being with 29.92% higher in the case of fish with a larger size. Theaverage number of red blood cell counts is constant for both experiments and these values are fallinginto the normal physiological gap. Erythrocyte constants (MCV, MCH and MCHC vary according to thetwo size classes: MCH grows by 31.53% and MCHC by 28.60% with the individual growth of the carp.MCV presents values between 186.48 and 199.73 (μm3, normal value for the studied species. Thepresent study outlines the fact that the main hematological parameters vary according to the size class,the results being related to the results obtained following the investigation of the metabolic profile in thelight of the technological parameters.

  7. Genetic Diversity and Sequence Variation of Mitochondrial COI Gene Segments of Qingshui River Cyprinus carpio%清水江鲤鱼线粒体COI基因序列变异及遗传多样性

    Institute of Scientific and Technical Information of China (English)

    向燕; 朱玲; 胡世然; 张宗慧

    2013-01-01

    To provide a molecular biological reference for phyletic evolution and diverse research on Qingshui River C.carpio,mitochondrial cytochrome oxidase subunit Ⅰ (COI) gene segments(about 900 bp) of the Qingshui River cyprinid were sequenced and analyzed.The results were as follows:the length of the targeted sequence varied from 898 to 902bp,and the percentage of A+T and G+C were 55.3% and 44.6% respectively; 33 polymorphic loci,which were consisted of 24 transitions,6 transversions and 3 transitions coexisted with transversions,were detected in the sequence of the population; The nucleotide diversity(Pi) and the average number of nucleotide differences(K) of the 38 individuals was 0.00451 and 4.038,respectively; All individuals were merged into 25 haplotypes; The haplotypic diversity(Hd) was 0.964,and the average genetic distance between all haplotypes was 0.005; In the neighbor joining(NJ)phylogenetic tree,all but one haplotypes clustered into two branches.All above indicated that the Qingshui River C.carpio exists abundant variation in COI sequence and relatively low genetic diversity.%为清水江鲤鱼的系统进化和多样性研究提供分子生物学依据,对清水江鲤鱼线粒体细胞色素氧化酶亚基Ⅰ(COI)基因序列的变异及遗传多样性进行了研究.结果表明:38个清水江鲤鱼个体中COI序列长度在898~902bp,碱基A+T和G+C的平均含量分别为55.3%和44.6%;在检测到的33个多态位点中,完全转换、完全颠换、转换与颠换共存位点数分别为24个、6个、3个;核苷酸多样性(Pi)和平均核苷酸差异数(K)分别为0.00451和4.038;38个个体分属25个单倍型,单倍型多样度(Hd)高达0.964,平均遗传距离为0.005;构建的邻接(NJ)系统树中,除1个单倍型外,其余24个聚为2个分支.清水江鲤鱼COI序列变异较丰富,但其遗传多样性较低.

  8. Clone, expression, purification and activity characterization of Jian carp(Cyprinus carpio var.Jian) Cathepsin L%建鲤组织蛋白酶L的克隆表达、纯化及活性特征鉴定

    Institute of Scientific and Technical Information of China (English)

    李冉; 陈治光; 蒋然然; 陈秀华; 李树红; 李新; 钟海霞; 但静

    2015-01-01

    首先采用TA克隆技术克隆建鲤组织蛋白酶L(Cathepsin L,CAT L)成熟肽基因片段并进行双酶切鉴定,进而构建表达载体CAT L-pET-30a并转入宿主菌E.coli BL21,经1 mmol/L异丙基-β-D-硫代吡喃半乳糖苷(IPTG)在37℃诱导2h表达重组CAT L蛋白.而后经尿素梯度洗涤和镍离子亲和层析纯化目的蛋白,并利用SDS-PAGE检测诱导效果和纯化过程.最后以荧光合成肽底物(Z-Phe-Arg-MCA)测活法鉴定建鲤重组CAT L的热稳定性、pH稳定性,以及鱼糜生产和冻藏中常用添加剂对其活性稳定性的影响.双酶切鉴定结果表明成功克隆了目的基因片段,与鲤鱼CAT L基因序列相似性为99.11%.SDS-PAGE分析表明经诱导、尿素梯度洗涤及亲和层析后,成功获得高度纯化目的蛋白,分子量约28 ku.活性鉴定结果表明重组CAT L在20~50℃及pH3.0~6.5范围内稳定;氯化钠、焦磷酸钠对重组CAT L活性的抑制作用呈现剂量依赖关系,而各浓度蔗糖、山梨醇则对其活性无明显作用.本研究成功克隆、表达和纯化了建鲤CAT L,并阐明了热、pH及鱼糜生产和冻藏中常用添加剂对该酶稳定性的不同影响.

  9. Molecular fingerprinting of the myxozoan community in common carp suffering Swim Bladder Inflammation (SBI) identifies multiple etiological agents

    Czech Academy of Sciences Publication Activity Database

    Holzer, Astrid S.; Hartigan, Ashlie; Patra, Sneha; Pecková, Hana; Eszterbauer, E.

    2014-01-01

    Roč. 7, AUG 28 2014 (2014), s. 398. ISSN 1756-3305 R&D Projects: GA AV ČR(CZ) M200961205; GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Cyprinus carpio carpio * swim bladder inflammation * fish disease * Myxozoa * molecular diagnostic * rDNA * in situ hybridisation Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 3.430, year: 2014

  10. Histological Investigations on an Intestinal Level in Common Carp, One Summer Old, Fed with Organic Selenium (Selplex Supplemented Fodder

    Directory of Open Access Journals (Sweden)

    Aurel Şara

    2011-10-01

    Full Text Available The goal of this research was to analyze the effect of organic Selenium (SelPlex on the intestinal mucosa and theimpact on the growth and consumption performances in one summer old common carp (Cyprinus carpio, Lausitzand Galitian varieties. The research were conducted on a number of 392 fish, Lausitz variety divided in 2 groups andon 192 fish, Galitian variety, divided into 2 groups. The fish were raised in 2 ponds (one pond for the control groupand one pond for the experimental group, in a semi-intensive polyculture system. The rearing conditions wereidentical for the 2 groups. The fodder for the experimental group was supplemented with Selenium (0.03mg/kgfodder. At the end of the experiment, the Selenium supplementation had a positive influence on the height of theintestinal villi. The experimental group (both varieties showed significant differences compared with the controlgroup. The mean height of the intestinal microvilli from the experimental group, Lausitz variety, was 2.328μm,0.95% bigger compared to the same variety of the control group. The Galitian variety showed a mean height of themicrovilli of 2.227μm, 0.72% biger than the same variety of the control group. This development of the villistructures in the experimental groups led to an increase in the absorption surface of the intestine which explains theaccelerated growth and the improvement of the feed conversion ratio.

  11. 青土霉素废水出水对锦鲤肝脏抗氧化酶活性和丙二醛含量的影响%Effects of Wastewater Effluent of Penicillin and Oxytetracycline on SOD,CAT Activities and MDA Content in Liver Tissue of Cyprinus carpio

    Institute of Scientific and Technical Information of China (English)

    吴志刚; 沈洪艳; 高吉喜; 王勇军; 赵秀梅; 尚亚楼

    2013-01-01

    An exposure experiment was made to study the effect of different concentrations of mixed wastewater effluent of penicillin and oxytetracycline on the activities of superoxide dismutase (SOD),catalase (CAT),and malondialdehyde (MDA) content in liver tissue of Cyprinus carpio.The results showed that mixed wastewater effluent of penicillin and oxytetracyline had little effect on SOD activity,significant induction only occurred in 1/16 φ(96 h LC5o) group on the eighth day.The CAT activity of the 1/16 φ(96 h LC50)and the 1/256 φ(96 h LC50) groups were similar,according to the "up-down-up" cyclical fluctuation law,the 1/4 φ(96 h LC50) group showed bimodal trends on the fourth day and the eighth day.The MDA content of the 1/256 φ(96 h LC50) group showed a single peak trend on the sixth day,the trends of the 1/16 and 1/4 φ(96 h LC50) groups showed "up-flat-up",on the early (2 d) and late (10 d) stage of exposure,the MDA content of the groups of all concentrations showed an effect-dosage relationship,the greater the exposure concentration,the stronger the oxidative damage to the body.The exposure experiment results suggested that CAT activity fluctuated markedly,it indicated the the body suffered from a strong oxidative damage,different levels of oxidative stress appeared in the liver tissue of Cyprinus carpio.When the wastewater concentration reached a certain level,the body oxidative damage was obvious.When the oxyteracycline concentration exceeded the load range of free radical scavenging,the significant oxidative damage would appear again.CAT activity and MDA content were more sensitive to mixed wastewater effluent of penicillin and oxytetracycline,it will be able to reflect the oxidation stress,so it is suitable for aquatic biological index of monitoring penicillin and oxytetracycline pollution in water environment.%采用暴露实验方法,研究青、土霉素混合废水出水不同暴露浓度对锦鲤肝脏组织中超氧化物歧化酶(SOD)和过氧化氢

  12. Effects of metal ions on cyprinid fish immune response: In vitro effects of Zn2+ and Mn2+ on the mitogenic response of carp pronephros lymphocytes

    International Nuclear Information System (INIS)

    Lymphocytes from the pronephros of carp (Cyprinus carpio L) have been subjected to transformation by mitogens, concanavalin A (Con A), phytohemagglutinin (PHA), and lipopolysaccharides (LPS), with Zn or Mn at varying concentrations. Addition of Zn2+ (10(-7) to 10(-3) M) to mitogen-stimulated T and B cells enhanced [3H]thymidine incorporation. Addition of 10(-5) M Zn2+ inhibited the response to Con A, PHA, and LPS. At this concentration, Zn was toxic. Addition of Mn2+ (10(-7) to 10(-3) M) to mitogen-stimulated lymphocytes enhanced [3H]thymidine incorporation. This effect was observed with Con A- and PHA-stimulated lymphocytes, but not with LPS-stimulated lymphocytes. In contrast, addition of 10(-1) M Mn2+ to lymphocyte cultures exerted an inhibitor on the response to Con A or to PHA, while the response to LPS was unaffected. Addition of 10(-1) M Mn2+ to Con A- or PHA-stimulated cultures at different times after initiation of stimulation indicated that Mn2+ was inhibitory only when it was added before the first 16 hr of cultures. The inhibition induced by 10(-1) M Mn2+ could be reversed by adding 2 mM CaCl2 to the culture

  13. Performance of orangefin labeo, Labeo calbasu (Hamilton 1822) as a component of polyculture system

    OpenAIRE

    Bibha Chetia Borah; Rimzhim Gogoi; Abdur Rahman

    2014-01-01

    This paper deals with the performance of orangefin labeo, Labeo calbasu (Hamilton, 1822) as a component of conventional composite carp culture system. The species was included as 50% substitution to common carp (Cyprinus carpio) in view of its omnivorous feeding habit and bottom dwelling nature. The total production was 0.55% higher in the experimental culture (with L. calbasu and Cyprinus carpio) than in the control (common carp only). The difference in the production was however not signifi...

  14. Effect of atrazine and chlorpyrifos on common carp's liver%毒死蜱和阿特拉津对鲤肝脏的影响

    Institute of Scientific and Technical Information of China (English)

    韩英; 郝其睿; 魏菁; 赵荣伟; 牟振波; 徐革锋; 刘洋

    2015-01-01

    探讨阿特拉津(Atrazine,ATR)、毒死蜱(Chlorpyrifos,CPF)及其混合物对鲤(Cyprinus carpio L.)肝脏和血液相关指标的影响。将实验鲤分别暴露于不同浓度的阿特拉津、毒死蜱及其混合物中,并分别于175、350和525 d采集其肝组织、血液等样品。结果表明,阿特拉津和毒死蜱在鲤肝组织中均有残留,残留量随暴露时间及浓度的增加而降低;与对照组比较,各染毒组碱性磷酸酶、谷丙转氨酶及血糖水平显著升高,总蛋白、白蛋白水平下降,抗氧化能力下降,且毒死蜱与阿特拉津混合后毒性作用更强,说明两者的毒性有叠加作用;染毒后,肝脏ER-α和VTG-II基因表达水平升高。结果显示,鲤肝脏对阿特拉津和毒死蜱有较强的代谢能力,阿特拉津和毒死蜱在鲤肝脏无富集作用;阿特拉津和毒死蜱使鲤肝功能受损,抗氧化能力降低;ER-α和VTG-II基因的高调表达显示出阿特拉津和毒死蜱对鲤亦有环境雌激素作用。%In this study our purpose is to discuss the effects of atrazine and chlorpyrifos and their mixture to related index of common carp's liver and blood. Exposeed experimental carps respectively in atrazine, chlorpyrifos and their mixture of different concentrations, and collected samples of common carp's liver and blood at 175, 350 and 525 d. Results showed that both atrazine and chlorpyrifos had re-sidual in common carp's liver and the residual quantity reduced with the exposure time and the concen-tration increasing. Compared to the control group, the alkaline phosphatase, glutamic-pyruvic transami-nase and blood glucose levels of each infected group had significant rise, while the total protein and al-bumin levels declined. Moreover, the toxicity of the mixture of atrazine and chlorpyrifos was stronger than that prior to the blending, which illustrated the toxicity of the two have superposition. After infected, the expression

  15. 温度和饲料蛋白质水平对松浦镜鲤幼鱼血清生化指标的影响%Effects of temperature and dietary protein content on the blood biochemical parameters in juvenile Songpu mirror carp Cyprinus specularis Songpu

    Institute of Scientific and Technical Information of China (English)

    黄金凤; 徐奇友; 王常安; 赵志刚; 罗亮

    2013-01-01

    The effects of water temperature (18, 23 ℃, and 28 ℃ ) and dietary protein levels (30% , 32% , 34% , 36% and 38%) on blood biochemical parameters were investigated in juvenile (body weight 10. 11 g±l. 07 g) Songpu mirror carp Cyprinus specularis Songpu reared in recirculating tanks at a rate of 30 individuals per tank with triplication for 60 d. The results showed that total protein (TP) and triglyceride (TG) levels in the juveniles were increased with the increase in dietary protein levels at 18 ℃, while glutamate-oxaloacetate transaminase (AST) activity decreased with the increase in dietary protein levels at 18 ℃. There were no significant differences in total cholesterol (CHOL) levels at all temperature (P>0.05) , and urea nitrogen (BUN), creatinine (CREA), blood glucose (GLU) , and albumin (ALB) levels, and glutamate-pyruvate transaminase (ALT) activity, and athero-genic lipoprotein profile (ALP) levels were increased then decreased with increase in dietary protein levels. There were no significant differences (P>0.05) in protein treatments. Under the same protein level, TG, BUN, CREA, TP, ALB, ALT and AST levels were significant decreased (P<0.05) with the increase in temperature, while ALP level were significant increased(P<0.05). At 23 ℃ , GLU and GGT levels were significant higher than that in the other temperature treatments. There was better nutritive metabolism of Songou mirror carp at 23 ℃ than at 18 and 28 X ,and the optimal dietary protein level was found to be 32% at 18 ℃ , 34% at 23 ℃ and 34% at 28 ℃.%研究了在不同温度(18、23、28℃)下,饲喂5种蛋白质水平(质量分数为30%、32%、34%、36%和38%)的饲料对松浦镜鲤Cyprinus specularis Songpu幼鱼(10.11 g±1.07 g)血清生化指标的影响.试验分为15组,每组设3个重复,每个重复放30尾鱼,饲养试验共进行60 d.结果表明:18℃下,鱼血清总蛋白(TP)和甘油三酯(TG)含量随饲料蛋白质水平的升高而升高,血清谷

  16. Patterns of metal composition and biological condition and their association in male common carp across an environmental contaminant gradient in Lake Mead National Recreation Area, Nevada and Arizona, USA.

    Science.gov (United States)

    Patiño, Reynaldo; Rosen, Michael R; Orsak, Erik L; Goodbred, Steven L; May, Thomas W; Alvarez, David; Echols, Kathy R; Wieser, Carla M; Ruessler, Shane; Torres, Leticia

    2012-02-01

    There is a contaminant gradient in Lake Mead National Recreation Area (LMNRA) that is partly driven by municipal and industrial runoff and wastewater inputs via Las Vegas Wash (LVW). Adult male common carp (Cyprinus carpio; 10 fish/site) were collected from LVW, Las Vegas Bay (receiving LVW flow), Overton Arm (OA, upstream reference), and Willow Beach (WB, downstream) in March 2008. Discriminant function analysis was used to describe differences in metal concentrations and biological condition of fish collected from the four study sites, and canonical correlation analysis was used to evaluate the association between metal and biological traits. Metal concentrations were determined in whole-body extracts. Of 63 metals screened, those initially used in the statistical analysis were Ag, As, Ba, Cd, Co, Fe, Hg, Pb, Se, Zn. Biological variables analyzed included total length (TL), Fulton's condition factor, gonadosomatic index (GSI), hematocrit (Hct), and plasma estradiol-17β and 11-ketotestosterone (11kt) concentrations. Analysis of metal composition and biological condition both yielded strong discrimination of fish by site (respective canonical model, p<0.0001). Compared to OA, pairwise Mahalanobis distances between group means were WBcarp collected along a contaminant gradient in LMNRA have distinct, collection site-dependent metal and morpho-physiological profiles that are significantly associated with each other. These associations suggest that fish health and reproductive condition (as measured by the biological variables evaluated in this study) are

  17. Umatilla - Rough Fish Eradication

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — In order to enhance environmental conditions in the McCormack Slough on Umatilla NWR, the population of rough fish, including common carp (Cyprinus carpio) and...

  18. Růst značkované násady kapra obecného (Cyprinus carpio) ve vybraných údolních nádržích v povodí řeky Moravy

    Czech Academy of Sciences Publication Activity Database

    Prokeš, Miroslav; Baruš, Vlastimil; Habán, V.; Mareš, J.; Peňáz, Milan; Halačka, Karel; Krejčí, R.; Vetešník, Lukáš

    Brno : Mendelova univerzita, 2014 - (Kopp, R.), s. 123-131 ISBN 978-80-7509-153-6. [65 let výuky rybářství na Mendelově univerzitě v Brně. Brno (CZ), 02.12.2014-03.12.2014] Institutional support: RVO:68081766 Keywords : common carp Subject RIV: GL - Fishing

  19. Růst značkované násady kapra obecného (Cyprinus carpio) ve vybraných údolních nádržích v povodí řeky Moravy

    Czech Academy of Sciences Publication Activity Database

    Prokeš, Miroslav; Baruš, Vlastimil; Habán, V.; Mareš, J.; Peňáz, Milan; Halačka, Karel; Krejčí, R.; Vetešník, Lukáš

    Brno: Mendelova univerzita, 2014 - (Kopp, R.), s. 123-131 ISBN 978-80-7509-153-6. [65 let výuky rybářství na Mendelově univerzitě v Brně. Brno (CZ), 02.12.2014-03.12.2014] Institutional support: RVO:68081766 Keywords : common carp Subject RIV: GL - Fishing

  20. Růst a časově-prostorová distribuce značkovaného kapra obecného Cyprinus carpio ve třech velmi rozdílných údolních nádržích v povodí řek Dyje, Jihlavy a Svratky (ČR)

    Czech Academy of Sciences Publication Activity Database

    Prokeš, Miroslav; Baruš, Vlastimil; Mareš, J.; Habán, V.; Peňáz, Milan

    Vodňany: Jihočeská univerzita, 2010 - (Vykusová, B.; Dvořáková, Z.). s. 25 [Česká ichtyologická konference /12./. 19.05.2010-20.05.2010, Vodňany] Institutional research plan: CEZ:AV0Z60930519 Keywords : common carp Subject RIV: GL - Fishing

  1. Crescimento de jundiá e carpa húngara criados em sistema de recirculação de água Jundiá growth and Common Carp reared in recirculated water system

    Directory of Open Access Journals (Sweden)

    Viviani Corrêia

    2009-08-01

    Full Text Available O presente estudo foi realizado com o objetivo de comparar o crescimento e o rendimento de carcaça e a composição corporal de juvenis de jundiá (Rhamdia quelen e carpa húngara (Cyprinus carpio criados em sistema de recirculação de água, em mono e bicultivo, durante 60 dias, na fase de recria. Cinco tratamentos com três repetições foram testados: 100C=100% carpa; 100J=100% jundiá; 50C50J=50% carpa 50% jundiá; 75C25J=75% carpa 25% jundiá e 25C75J=25% carpa 75% jundiá, sendo utilizados 20 peixes por unidade experimental. Foram avaliados o peso (P, a biomassa total (BT, o fator de condição (FC, a conversão alimentar aparente (CAA, a taxa de crescimento específico (TCE, o rendimento de carcaça (RC, o índice digestivo-somático, o índice hepato-somático, o quociente intestinal, as composições centesimais dos peixes, as taxas de deposição de proteína e a gordura corporal. Não foram observadas diferenças no peso, no FC e na TCE dos jundiás (P>0,05. Para essas variáveis, as carpas submetidas ao tratamento 25C75J apresentaram valores significativamente maiores (PA study was conducted to evaluate growth, carcass yield and body composition of jundiá and common carp raised in mono or duoculture in a recirculated water system, for 60 days, during the growing. Five treatments were tested with three replicates: 100C=100% carp; 100J=100% jundiá, 50C50J=50% carp 50% jundiá; 75C25J=75% carp 25% jundiá and 25C75J=25% carp 75% jundiá. Twenty fishes were distributed in each experimental unit. It was evaluated weight (W, total biomass (TB, condition factor (CF, feed conversion rate (FCR, specific growth rate (SGR, carcass yield (YC, digestive-somatic index, hepatossomatic index, intestinal quotient and the proximate composition and body deposition of protein and fat. Considering jundiá performance, no statistical difference was observed for W, CF and SGR (P>0.05. Common carp from treatment 25C75J showed the highest values (P<0

  2. The cestode Atractolytocestus huronensis (Caryophyllidea) continues to spread in Europe: new data on the helminth parasite of the common carp

    Czech Academy of Sciences Publication Activity Database

    Oros, M.; Hanzelová, V.; Scholz, Tomáš

    2004-01-01

    Roč. 62, 1/2 (2004), s. 115-119. ISSN 0177-5103 R&D Projects: GA ČR GA524/04/0342 Grant ostatní: GA SR(SK) VEGA2/4177/04 Institutional research plan: CEZ:AV0Z6022909 Keywords : Atractolytocestus huronensis * Cyprinus carpio * man-made introduction Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.583, year: 2004

  3. Fontes protéicas vegetais na alimentação da carpa húngara Plant protein sources on common carp feeding

    Directory of Open Access Journals (Sweden)

    Giovani Taffarel Bergamin

    2011-09-01

    Full Text Available A substituição parcial da farinha de carne suína por farelos vegetais em dietas para juvenis de carpa húngara (Cyprinus carpio foi avaliada em cinco dietas experimentais: um controle (farinha de carne suína (FCS como fonte protéica e quatro dietas vegetais: farelo de soja (FS, farelo de canola (FC, farelo de girassol (FG ou farelo de linhaça (FL, substituindo 50% da proteína proveniente da FCS. 195 juvenis (43,4±0,4g foram alimentados duas vezes ao dia (9 e 15 horas durante 71 dias. Ao final do experimento, maior peso (205,69g, ganho em peso (2,29g dia-1, taxa de crescimento específico (2,2% dia-1 e conversão alimentar (1,74 foram obtidos nas dietas com FCS, seguido dos tratamentos FC e FS que não diferiram entre si. Os peixes alimentados com FCS, FG e FS apresentaram maior gordura corporal. A dieta FL apresentou piores resultados de desempenho, coeficiente de retenção protéica, deposição de proteína e gordura corporal e no filé. Pode-se concluir que a dieta à base de farinha de carne suína proporciona maior crescimento e deposição de proteína em juvenis de carpa húngara, comparada às dietas com farelos vegetais; os parâmetros de carcaça (rendimentos e índices digestivos não são afetados pelas fontes protéicas da dieta.This study was conducted to evaluate the partial replacement of porcine meat meal by plant-protein meals in diets for common carp (Cyprinus carpio. Five experimental diets were evaluated: control (FCS, only porcine meat meal as protein source, and four plant-based diets: soybean meal (FS, canola meal (FC, sunflower meal (FG or linseed meal (FL replacing 50% of protein from porcine meat meal. Juveniles (195, 43.4±0.4g were fed twice daily (9:00 am and 03:00pm for 71 days. At the end of the trial, the best weight (205,69g, weight gain (2,29g day-1, specific growth rate (2,2% day-1 and food conversion ratio (1,74 were obtained in FCS, followed by FC and FS, that did not differ among each other. Fish

  4. Individuální růst a hmotnostní kondice kapra obecného (Cyprinus carpio m. domestica) v Novomlýnské nádrži (předběžné výsledky)

    Czech Academy of Sciences Publication Activity Database

    Habán, V.; Prokeš, Miroslav; Baruš, Vlastimil; Mareš, J.

    České Budějovice : Jihočeská Univerzita, 2006 - (Vykusová, B.), s. 16-21 ISBN 80-85887-57-6. [Česká ichtyologická konference /9./. Vodňany (CZ), 04.05.2006-05.05.2006] R&D Projects: GA MZe(CZ) QF3028 Institutional research plan: CEZ:AV0Z60930519 Keywords : common carp * growth rate Subject RIV: GL - Fishing

  5. A Study on the Metabolic Residue Rule of Norfloxacin Nicotinate in Common Carp%烟酸诺氟沙星在松浦镜鲤体内代谢残留规律的研究

    Institute of Scientific and Technical Information of China (English)

    段可馨; 韩冰; 王荻; 卢彤岩

    2016-01-01

    分析烟酸诺氟沙星在松浦镜鲤体内代谢残留规律,准确制定该种药物的休药期,为有效、安全、合理地使用烟酸诺氟沙星提供准确科学的理论依据。选用松浦镜鲤( Cyprinus carpio L.)为实验对象,连续5 d 口灌30 mg/(kg·d)烟酸诺氟沙星(norfloxacin nicotinate,NFX)后,分别于给药后1,2,3,4,5,6,7,9,11,13,15,20,25 d采集鲤血浆、肌肉、皮、肝和肾5种组织,通过高效液相色谱法检测其各组织内烟酸诺氟沙星的残留量。烟酸诺氟沙星在鲤体内吸收分布迅速,代谢较为缓慢,各组织分布量由多到少依次为:肾脏、肝胰脏、肌肉、皮肤、血浆,为避免组织内残留过多药物,建议休药期为24 d。%The aim of this study is to study the residual rule of norfloxacin nicotinate in common carp,for⁃mulate the withdrawal time of norfloxacin accurately and provide a precision and scientific theory basis for u⁃sing this medicine reasonably,safely and effectively.In the experiment,common carp(Cyprinus carpio L.)was taken as the target,the fish were drenched with the medicine 30 mg/( kg ·d) for 5 days,then their kidney,liv⁃er,muscle,skin and blood were collected at the 1st,2nd,3rd,4th,5th,6th,7th,9th,11th,13th,15th,20th,25thday af⁃ter the medication.The residual of norfloxacin nicotinate in the above 5 kinds of tissues was detected by high performance liquid chromatography(HPLC).Norfloxacin nicotinate was absorbed by the tissues rapidly but me⁃tabolized slowly.The distribution of the medicine was in the order:kidney>liver>muscle>skin>blood.In order to avoid excessive medicine residues within the tissues,the 24 days of withdrawal time is proposed.

  6. Potted Silver Carp

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    Ingredients: 4 silver carp of about 200 grams each, mushrooms, winter bamboo shoots, minced ginger root and scallions, cooking wine, broth, salt and MSG (optional). Method: 1. After cleaning the carp place them in a pot; add in all other ingredients and some broth, Seal the opening of the pot with kraft paper

  7. Contrast Test of the Economic Benefit of the Pond Fish- farming Modes Taking the Grass Carp and the Cyprinus Carpio as the Main Fish Seed Respectively%池塘主养草鱼与主养鲤鱼模式经济效益的对比试验

    Institute of Scientific and Technical Information of China (English)

    张志远; 续满光; 任惠民

    2004-01-01

    试验表明:池塘养殖草鱼的比较效益明显高于养殖鲤鱼.总结出一些池塘主养革鱼规范化的技术操作模式,解决了池塘主养鲤鱼效益低下的矛盾,提高了单位水体的经济效益.

  8. Dissemination of spring viraemia of carp (SVC in Serbia during the period 1992-2002

    Directory of Open Access Journals (Sweden)

    Jeremić Svetlana

    2004-01-01

    Full Text Available An acute contagious viral disease caused by Rhabdovirus carpio, spring viraemia of carp, was described for the first time by Fijan in 1972. Carp is the most important susceptible species in all age categories, although other species of cyprinid fish also contract the disease. As the name suggest, the disease typically appears in spring, when the water temperature increases. The disease is often complicated by the secondary appearance of bacterial and parasitic infections. The clinical symptoms include and presume gathering of infected fish near the sides of the pond, uncoordinated swimming, darkening of body color, pale gills, petechial bleeding (of the skin, eyes, gills, and fin bases, exophthalmus, and the appearance of pseudofecal expulsions from the infected anus. Pathoanatomical examination sometimes reveals only oedematous organ enlargement and intestinal inflammation. A varying degree of haemorrhaging is present in the bladder, internal organs, and fluid-containing cavities. Diagnosis is based on isolation and identification of the virus and ELISA in the laboratory. More than 1664 samples were analyzed in the course of the 10-year investigation (from 1992-2002 from different carp hatchery localities in the Republic of Serbia. Rhabdovirus carpio was isolated and identified at more than a third of the tested fish hatcheries (31,57% infected with the spring viraemia virus. Although the disease was known earlier, it now represents an urgent problem in many European countries, and has been causing great damage in intensive and semi-intensive carp production in our country during the last few years. Therefore, special attention has been paid to it.

  9. Isolation and expression analysis of Sox10 gene relating body color variation in Oujiang color common carp%瓯江彩鲤体色相关基因Sox10的分离与表达分析

    Institute of Scientific and Technical Information of China (English)

    李康乐; 胡建尊; 颜标; 王成辉

    2013-01-01

    The numerous and diverse body color of fish was not only excellent materials to study the molecular mechanism of body color and body color inherence, body color evolution, and it was also an ideal model to diagnosis disease. Now, five basic and stable body color patterns have existed in Oujiang color common carp (Cyprinus carpio var. color), namely“Quanhong”,“Dahua”,“Mahua”,“Fenyu”and“Fenhua”, respectively. This fish can be a very good model and material for studying body color inheritance. At the melanin synthesis signaling pathway, skin color was regulated by a small group genes. Sox10 gene plays an important role in the formation of melanin, it regulates the birth, migration and differentiation of melanin. However, the effect of Sox10 gene on body color has not been reported. Four healthy lines of Oujiang color carp (“Quanhong”, “Dahua”, “Fenyu”,“Fenhua”) were collected from the provincial farm of the Zhejiang. Then the skin, muscle, eye, gill, kidney, swim bladder, heart, and liver were taken out, furthermore, the black skin and red skin were sampled separately of“Dahua”, the same with black skin and white skin of“fenhua”. Total RNA was extracted using Trizol, and reverse transcribed into the First-strand cDNA. Primers were designed by Primer5 program, and the right products were purified and sequenced. The obtained sequence was analyzed by Bioedit, Clustalw, Mega and other bioinformatics softwares. The total length of Sox10 gene cloned in Oujiang color common carp is 2 830 bp which contains 9 bp 5’ untranslated regions (UTR) and 1 375 bp 3’-UTR, and 1 446 bp open reading frame (ORF) encoding 481 amino acids. The phylogenetic analyses showed that there were 59%−94%similarities in amino acid sequence with some of previously reported other species. RT-PCR analysis revealed that the gene was highly expressed in skin, muscle, eyes and swim bladder, weakly expressed in gill and heart and no expression were found in

  10. Časoprostorová distribuce úlovků, růst a délkohmotnostní vztah u značkovaného kapra obecného (Cyprinus carpio) v rybářském revíru MRS Dyje 5, Novomlýnská nádrž a přilehlých revírech

    Czech Academy of Sciences Publication Activity Database

    Prokeš, Miroslav; Baruš, Vlastimil; Mareš, J.; Habán, V.; Peňáz, Milan

    Brno : MZLU, 2009 - (Kopp, R.), s. 22-29 ISBN 978-80-7375-358-0. [60 let výuky rybářské specializace na Mendelově zemědělské a lesnické univerzitě v Brně. Brno (CZ), 02.12.2009-03.12.2009] R&D Projects: GA MZe QH71305 Institutional research plan: CEZ:AV0Z60930519 Keywords : common carp * Nové Mlýny reservoirs Subject RIV: GL - Fishing

  11. 活体和施肥管理体系下锦鲤池塘产量、水质及细菌学参数%Fish Production,Water Quality and Bacteriological Parameters of Koi Carp Ponds Under Live-food and Manure Based Management Regimes

    Institute of Scientific and Technical Information of China (English)

    Prithwiraj Jha; Sudip Barat; Chitta R.Nayak

    2008-01-01

    为探讨在观赏池塘中的投放浮游动物以及直接投放动物粪便对锦鲤(Cyprinus carpio L.)的生长及产量的影响,在池塘中进行了为期11周的实验.实验按如下四种管理系统进行处理:1.给幼体锦鲤投喂浮游动物饲料(LF组);2.直接投放家禽粪便(PM组);3.直接投放牛粪(CD组);4.不投放任何食物,仅进行常规管理(C组).每组实验重复三次.同时检测非自养细菌及致病微生物(如:Aeromonas sp.和Pseudomonas sp.)的生长状况,以此了解池塘的管理状况.在LF组中,其水体含氧量较高,与其它组相比具显著差异(P<0.05).而PM、CD组与LF、C组比较,在PO4-P,NH4-N,NO3-N,NO2-N的关系,导电率、碱度以及生化需氧量等较高,且差异显著(P<0.05),在池塘底部淤泥中的总氮量及有机碳百分率方面PM、CD与LF组相比,具有显著差异(P<0.05).PM与CD组与其它组相比在池塘中的非自养细菌(Aeromonas sp.和Pseudomonas sp.)的繁殖率较高,皆具显著差异P<0.05).LF组中锦鲤的体重增长率较其它组高(P<0.05).锦鲤幼体在C及LF组中的成活率分别为:67.21%和90.11%.结果提示:提高锦鲤幼体的存活率及其产量可通过对水质的管理(即保持优良水质)及提高池塘中浮游生物丰富度加以获得.值得注意的是:LF组中非自养细菌(Aeromonas sp.与Pseudomonas sp.)比率的过低将导致细菌性疾病的发生.%To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production,larvae of koi carp,Cyprinus carpio L.,were cultured for 11 weeks in earthen ponds maintained according to four management regimes:(1)live zooplankton fed to carp lalwae(LF);(2)direct fertilization with poultry manure(PM);(3)direct fertilization with cowdung(CD);and(4)a conffol treatment(C).There were three replicates for each treatment.The growth of heterotrophic bacteda and pathogenic microorganisms like Aeromonas sp.and Pseudomonas sp

  12. Toxicity of Diazinon 60 EC for Cyprinus carpio and Poecilia reticulata

    Czech Academy of Sciences Publication Activity Database

    Máchová, J.; Prokeš, Miroslav; Svobodová, Z.; Žlábek, V.; Peňáz, Milan; Baruš, Vlastimil

    2007-01-01

    Roč. 15, č. 3-4 (2007), s. 267-276. ISSN 0967-6120. [New Challenges in Pond Aquaculture . České Budějovice, 26.04.2005-28.04.2005] R&D Projects: GA MZe QF3029 Institutional research plan: CEZ:AV0Z60930519 Keywords : acute toxicity * chemical insecticide * Daphnia magna * embryonic toxicity test * larval toxicity test * organophosphate * pond fishery Subject RIV: GL - Fishing Impact factor: 0.828, year: 2007

  13. Imaging a spinal fracture in a Kohaku Koi (Cyprinus carpio): techniques and case history report

    International Nuclear Information System (INIS)

    An ornamental pet fish was diagnosed with a spinal fracture and subluxation involving truncal vertebrae 5 and 6 (T5-T6) using conventional radiography, nuclear scintigraphy, and computed tomography. Attempts to evaluate the dynamic nature of the lesion using conventional fluoroscopy in the unanesthetized, moving patient were? unsuccessful, Adaptation of imaging techniques to accommodate a fish patient was not difficult and diagnostic images were obtained. The use of multiple imaging techniques was useful in the diagnosis and determination of the treatment plan of the spinal fracture in this patient

  14. RESPONSE OF CYPRINUS CARPIO TO PHENOL AND FURFURAL SHOCK LOADS IN PACT SYSTEM

    OpenAIRE

    A. Mesdaghinia; N. Djaafarzadeh; S Nasseri

    1999-01-01

    Treatment efficiency, design factors and kinetic coefficients were studied using two pilots of activated sludge (AS) and powdered activated carbon technology (PACT), for treating Tehran Oil Refinery effluent, in a duration of more than 12 months In order to evaluate the performance of each system against growth inhibitor shock loads, different concentrations of phenol and furfural (10-300 mg/l) were applied, following a series of experiments in which, treated effluents from the two systems we...

  15. Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis

    Czech Academy of Sciences Publication Activity Database

    Bazsalovicsová, E.; Kráľová-Hromadová, I.; Štefka, Jan; Scholz, Tomáš

    2012-01-01

    Roč. 110, č. 5 (2012), s. 1621-1629. ISSN 0932-0113 R&D Projects: GA ČR GA524/08/0885; GA MŠk LC522 Institutional support: RVO:60077344 Keywords : INTERNAL TRANSCRIBED SPACER-1 * RIBOSOMAL- RNA GENES * SEQUENCE VARIATION * CYPRINUS-CARPIO * INTRAGENOMIC HETEROGENEITY * TAPEWORMS PLATYHELMINTHES * RDNA * EVOLUTION * POPULATION Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.852, year: 2012 http://link.springer.com/article/10.1007%2Fs00436-011-2673-0

  16. Biomarker Benchmarks: Reproductive and Endocrine Biomarkers in Largemouth Bass and Common Carp from United States Waters

    Science.gov (United States)

    Goodbred, Steven L.; Smith, Stephen B.; Greene, Patricia S.; Rauschenberger, Richard H.; Bartish, Timothy M.

    2007-01-01

    The U.S. Geological Survey (USGS) has developed a national database and report on endocrine and reproductive condition in two species of fish collected in U.S. streams and rivers. This information provides scientists with a national basis for comparing results of endocrine measurements in fish from individual sites throughout the country, so that scientists can better ascertain normal levels of biomarkers. The database includes information on several measures of reproductive and endocrine condition for common carp and largemouth bass. Data summaries are provided by reproductive season and geographic region. A national-scale reconnaissance investigation was initiated in 1994 by the USGS that utilized a suite of biological assays (biomarkers) as indicators of reproductive health, and potentially, endocrine disruption in two widely distributed species of teleost (bony) fish, largemouth bass (Micropterus salmoides) and common carp (Cyrinus carpio). The suite of assays included plasma sex-steroid hormones, stage of gonadal development, and plasma vitellogenin, an egg protein that indicates exposure to estrogenic compounds when found in male fish. More than 2,200 common carp and 650 largemouth bass were collected at 119 rivers and streams (fig. 1).

  17. Larval gryporhynchid tapeworms (Cestoda: Cyclophyllidea) of British freshwater fish, with a description of the pathology caused by Paradilepis scolecina

    Czech Academy of Sciences Publication Activity Database

    Williams, C. F.; Reading, A. J.; Scholz, Tomáš; Shinn, A. P.

    2012-01-01

    Roč. 86, č. 1 (2012), 1-9. ISSN 0022-149X R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : CARP CYPRINUS-CARPIO * METACESTODES * DILEPIDIDAE * PARASITES * PLEROCERCOIDS * MOZAMBIQUE * INFECTION * LINNAEUS * LAKE Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.157, year: 2012

  18. Stress physiology in fish : central regulation and organismal adaptions

    NARCIS (Netherlands)

    Metz, Juriaan Rogier

    2006-01-01

    The main goal of this thesis was to investigate the central regulation of the stress 428042response and consequent organismal adaptations in the common carp, Cyprinus carpio. In chapter 2 the control by CRH of ACTH release from the pituitary PD is investigated. It further describes the immunohistoch

  19. Current lineages of the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells

    Science.gov (United States)

    Winton, J.; Batts, W.; deKinkelin, P.; LeBerre, M.; Bremont, M.; Fijan, N.

    2010-01-01

    Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, Sulimanovic, Bearzotti, Muzinic, Zwillenberg, Chilmonczyk, Vautherot & de Kinkelin 1983) has become one of the most widely used tools for research on fish viruses and the diagnosis of fish viral diseases.

  20. The role of ecological context and predation risk-stimuli in revealing the true picture about the genetic basis of boldness evolution in fish

    DEFF Research Database (Denmark)

    Klefoth, Thomas; Skov, Christian; Krause, Jens; Arlinghaus, Robert

    2011-01-01

    To showcase the importance of genotype × environment interactions and the presence of predation risk in the experimental assessment of boldness in fish, we investigated boldness in terms of feeding behavior and refuge use in two genetically different populations of juvenile carp (Cyprinus carpio...

  1. Cytocomposition of the vitellarium in Khawia sinensis Hsü, 1935 (Cestoda, Caryophyllidea, Lytocestidae): another caryophyllidean species with lamellar bodies and lipids

    Czech Academy of Sciences Publication Activity Database

    Bruňanská, M.; Drobníková, P.; Mackiewicz, J. S.; Nebesářová, Jana

    2013-01-01

    Roč. 112, č. 7 (2013), s. 2703-2711. ISSN 0044-3255 Grant ostatní: ERDF(XE) 26220120022 Institutional support: RVO:60077344 Keywords : Wenyonia virilis * Carp cyprinus-carpio * reproductive system * vitteline cells * ultrastructure * phylogenetic implications Subject RIV: EA - Cell Biology

  2. Consequences of the size structure of fish populations for their effects on a generalist avian predator

    OpenAIRE

    Kloskowski, Janusz

    2010-01-01

    Size-structured interspecific interactions can shift between predation and competition, depending on ontogenetic changes in size relationships. I examined the effects of common carp (Cyprinus carpio), an omnivorous fish, on the reproductive success of the red-necked grebe (Podiceps grisegena), an avian gape-limited predator, along a fish size gradient created by stocking distinct age-cohorts in seminatural ponds. Young-of-the-year (0+) carp were an essential food source for young grebes. Only...

  3. The tapeworm Atractolytocestus tenuicollis (Cestoda: Caryophyllidea) - a sister species or ancestor of an invasive A. huronensis?

    Czech Academy of Sciences Publication Activity Database

    Kraľová-Hromadová, I.; Štefka, Jan; Bazsalovicsová, E.; Bokorová, S.; Oros, M.

    2013-01-01

    Roč. 112, č. 10 (2013), s. 3379-3388. ISSN 0932-0113 R&D Projects: GA ČR GBP505/12/G112 Grant ostatní: Centre of Excellence for Parasitology(XE) 2622012011 Institutional support: RVO:60077344 Keywords : carp Cyprinus carpio * farmed common carp * Cestoda Caryophyllidea * sequence alignment * parasites * fishes Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.327, year: 2013

  4. 瓯江彩鲤体色调控相关因子MC1R的克隆与表达分析%Cloning and expression analysis of color-related regulation factor MC1R in Oujiang color common carp

    Institute of Scientific and Technical Information of China (English)

    胡建尊; 李康乐; 项松平; 王剑; 朱丽艳; 王成辉

    2013-01-01

    黑素皮质素1受体(melanocortin 1 receptor,MCIR)是动物黑色素合成通路中的关键基因之一,对动物的体色和毛色有重要影响.瓯江彩鲤(Cyprinus carpio var.color)具有5种基本体色类型(“全红”、“粉玉”、“大花”、“粉花”和“麻花”),其中后3种带有黑色斑点或斑块,是研究动物黑色素合成机制的良好材料之一.克隆了瓯江彩鲤的MC1R基因,并进行了“全红”、“大花”、“粉玉”、“粉花”4种体色间的表达差异分析.研究发现,瓯江彩鲤的MC1R基因的全长cDNA序列为1 914 bp,包括637 bp 5’-UTR(非转录区)、311 bp3’-UTR及966 bp ORF(开放阅读框).该基因由一个外显子编码(321个氨基酸),有7个跨膜结构域.瓯江彩鲤与鲫的编码区核苷酸同源性达98%,与斑马鱼达93%;不同体色间的编码区只存在一个核苷酸无义突变(C/G),因而氨基酸序列完全相同.qRT-PCR表明,MC1R基因在眼睛的表达量显著高于皮肤、肌肉、鳃、肾脏、鳔、心、肝等组织(P<0.05);但在4种体色间不存在显著的表达差异(P>0.05).研究结果表明:瓯江彩鲤体色类型中的黑色斑块不是由MC1基因直接决定,还有待对其它体色相关基因或调控因子的研究.研究亮点:对在黑色素合成通路中起着类似“开关”作用的MC1R基因进行了克隆、序列分析和瓯江彩鲤体色间的表达差异分析,排除了MC1R基因与瓯江彩鲤黑色斑纹的直接相关性,为鱼类体色变异相关基因研究积累了有益资料.%Melanocortin 1 receptor (MC1R),one of the key genes in animal melanin synthesis pathway,plays an important role in determining animal body color and coat color.Oujiang color common carp (Cyprinus carpio var.color),characterized as five kinds of body color patterns with black patches (e.g."whole red","whole red with big black patch","whole red with scattered black spots"," whole white" and "whole white with big black patch

  5. 草鱼胰岛素样生长因子1受体基因cDNA全序列的克隆及功能%cDNA cloning and function of insulin-like growth factor-I receptor gene (Igf-Ir) in grass carp (Ctenopharyngodon idellus)

    Institute of Scientific and Technical Information of China (English)

    周春雪; 蒋霞云; 陈杰; 邹曙明

    2014-01-01

    本研究采用cDNA末端快速扩增法(RACE)克隆了草鱼(Ctenopharyngodon idellus)IGF-IR基因全长cDNA序列,并对该基因在草鱼不同时期胚胎和成鱼不同组织中的表达进行了分析。序列分析表明,草鱼IGF-IR基因cDNA序列全长5741 bp,包括5′端非翻译区822 bp,3′端非翻译区581 bp,开放阅读框4338 bp,共编码1445个氨基酸。序列比对结果显示,草鱼 IGF-IR 可能属于 a 型,该基因编码的氨基酸序列与鲤(Cyprinus carpio)IGF-IRa、斑马鱼(Danio rerio)IGF-IRa和人类(Homo sapiens)IGF-IR的相似性分别为95%、93%和66%,具有较高的同源性,表明该基因在长期进化中具有较高的保守性。RT-PCR结果表明,该基因从16 hpf(hours post fertilization)胚胎期到出苗期都有表达,在成鱼大部分组织中均有表达。原位杂交结果显示,草鱼IGF-IR mRNA在不同时期胚胎组织中广泛存在,其中在脑部、脊索和尾部等生长旺盛组织的细胞中表达量较高。本研究为进一步探索草鱼IGF-IR基因在生长发育信号通路中的作用和育种提供了基础资料。%The insulin-like growth factor-I receptor (IGF-IR) is an important component of the insulin-like growth fac-tor system, which plays a key role in embryonic growth, formation of the nervous system, skeletal muscle development, and also appears to contribute to the establishment and progression of tumors. Despite its importance, little is known about the tissue distribution and expression pattern of IGF-IR during embryonic development in most commercially important cultured fish species. We isolated a 5 741 bp IGF-IR full-length cDNA from grass carp(Ctenopha ryngodon idellus). The IGF-IR cDNA included an 822 bp complete 5′untranslated region (5′UTR), a 581 bp 3′UTR, and a 4 338 bp open reading frame (ORF) region which encoded a 1 445 amino acid protein. The mature peptide contained seven structural domains including two Recep-L-domains, one Furin-like domain, three FN3

  6. Gene Expression Profiling of Grass Carp (Ctenopharyngodon idellus) and Crisp Grass Carp

    OpenAIRE

    Ermeng Yu; Jun Xie; Guangjun Wang; Deguang Yu; Wangbao Gong; Zhifei Li; Haiying Wang; Yun Xia; Nan Wei

    2014-01-01

    Grass carp (Ctenopharyngodon idellus) is one of the most important freshwater fish that is native to China, and crisp grass carp is a kind of high value-added fishes which have higher muscle firmness. To investigate biological functions and possible signal transduction pathways that address muscle firmness increase of crisp grass carp, microarray analysis of 14,900 transcripts was performed. Compared with grass carp, 127 genes were upregulated and 114 genes were downregulated in crisp grass c...

  7. Utilisation of vegetable leaves for carp production

    OpenAIRE

    Roy, A. K.; Rai, S.P.; Datta, A.K.; Das, C. R.; Ghosh, J. K.

    1996-01-01

    The results of two sets of experiments on mono-culture of grass carp (Ctenopharyngodon idella) and mixed culture of carps (grass carp 50 : catla 20 : rohu 15 : mrigal 15) fed exclusively with vegetable leaves are reported. The experiments were conducted with two replicates each in 0.02 ha ponds of Wastewater Aquaculture Division of the Central Institute of Freshwater Aquaculture, Rahara during 1991-93. Monoculture of grass carp stocked at 1000/ha demonstrated an average net production of 21.0...

  8. Prevalence of Argulus foliaceus in ornamental fishes [goldfish (Carassius auratus) and Koi (Cyprinus carpio)] in Kerman, southeast of Iran.

    Science.gov (United States)

    Mirzaei, M; Khovand, H

    2015-12-01

    The genus Argulus (Crustacea: Branchiura), or fish louse, are common parasites of freshwater fish. This parasite have a direct life cycles and mature females leave the host and lay several hundred eggs on vegetation and various objects in the water. It caused pathological changes due to direct tissue damage and secondary infections. Besides the damage and stress caused by Argulus itself, one of the main worries for fish producers are the associated secondary infestations and infections that can result from infestation with this ecto-parasite. From 300 samples, only 20 (6.67 %) samples were infested with this parasite and 280 (93.33 %) not infested. In the present study, Argulus foliaceus was reported on goldfish and Koi which this was first recorded in Kerman, southeast of Iran. According to the presented study, it is clear that A. foliaceus can act as a potential risk factor for natural ecosystems and native fish population of Iran and other countries, that should be mentioned to prevent the burst of new parasitic fauna to Iran and different countries as well as stop direct economic losses caused by mortality derived from infestation with this ecto-parasite. PMID:26688652

  9. Neurotoksičnost fumonizina B1 u šaranske mlađi (Cyprinus carpio L.)

    OpenAIRE

    Kovačić, Sanja; Pepeljnjak, Stjepan; Pertinec, Zdravko; Šegvić Klarić, Maja

    2009-01-01

    Odavno je poznato da okoliš ima važnu ulogu u razvoju neurodegenerativnih bolesti kao što su Alzheimerova i Parkinsonova bolest te multipla skleroza. Mikotoksin fumonizin B1 (FB1) tvori nekoliko vrsta Fusariuma, najčešće F. verticillioides, koja je najučestaliji kontaminant kukuruza. Ovaj mikotoksin odgovoran je za leukoencefalomalaciju konja, mula i magaradi povezanu s konzumacijom kukuruza kontaminiranog s FB1. U ovom su radu prikazani rezultati kronične eksperimentalne toksikoze mlađ...

  10. Identification of antibiotic resistance cassettes in class 1 integrons in Aeromonas spp. strains isolated from fresh fish (Cyprinus carpio L.).

    Science.gov (United States)

    Sarria-Guzmán, Yohanna; López-Ramírez, María Patricia; Chávez-Romero, Yosef; Ruiz-Romero, Erick; Dendooven, Luc; Bello-López, Juan Manuel

    2014-05-01

    Forty-six Aeromonas spp. strains were isolated from fresh fish and investigated for their antimicrobial susceptibility, detection of Class 1 integrons by PCR, and arrangement of gene cassettes. Selected isolates were further characterized by enterobacterial repetitive intergenic consensus-PCR. Twenty isolates were found to carry Class 1 integrons. Amplification of the variable regions of the integrons revealed diverse bands ranging in size from 150 to 1,958 pb. Sequence analysis of the variable regions revealed the presence of several gene cassettes, such as adenylyl transferases (aadA2 and aadA5), dihydrofolate reductases (dfrA17 and dfrA1), chloramphenicol acetyl transferase (catB3), β-lactamase (oxa2), lincosamide nucleotidil transferase (linF), aminoglycoside-modifying enzyme (apha15), and oxacillinase (bla OXA-10). Two open reading frames with an unknown function were identified as orfC and orfD. The aadA2 cassette was the most common integron found in this study. Interestingly, five integrons were detected in the plasmids that might be involved in the transfer of resistance genes to other bacteria. This is a first report of cassette encoding for lincosamides (linF) resistance in Aeromonas spp. Implications on the incidence of integrons in isolates of Aeromonas spp. from fresh fish for human consumption, and its possible consequences to human health are discussed. PMID:24370627

  11. CONSERVAÇÃO DO SÊMEN DA CARPA COMUM, Cyprinus carpio, VARIEDADE ORNAMENTAL, POR RESFRIAMENTO

    OpenAIRE

    Nilsa Hoffmann PADUA; Eduardo SHIMODA; Paula de Sousa BARBOSA; Geraldo PEREIRA JUNIOR

    2015-01-01

    O objetivo deste trabalho foi verificar a viabilidade da conservação do sêmen da carpa comum, variedade ornamental, sob resfriamento a 4 ºC. Foram utilizados oito peixes, vertendo sêmen mediante massagem abdominal. Após a aplicação de extrato bruto de hipófise de carpa, as amostras seminais foram extrusadas, sendo descartadas as contaminadas com sangue, fezes e/ou urina e as que apresentaram motilidade inferior a 80%. Cada amostra foi dividida em dois tubos de ensaio, sendo um deles mantido e...

  12. CONSERVAÇÃO DO SÊMEN DA CARPA COMUM, Cyprinus carpio, VARIEDADE ORNAMENTAL, POR RESFRIAMENTO

    Directory of Open Access Journals (Sweden)

    Nilsa Hoffmann PADUA

    2015-12-01

    Full Text Available O objetivo deste trabalho foi verificar a viabilidade da conservação do sêmen da carpa comum, variedade ornamental, sob resfriamento a 4 ºC. Foram utilizados oito peixes, vertendo sêmen mediante massagem abdominal. Após a aplicação de extrato bruto de hipófise de carpa, as amostras seminais foram extrusadas, sendo descartadas as contaminadas com sangue, fezes e/ou urina e as que apresentaram motilidade inferior a 80%. Cada amostra foi dividida em dois tubos de ensaio, sendo um deles mantido em temperatura ambiente (25 ± 3 ºC e o outro mantido sob resfriamento (4 ± 2 °C. Para análise da motilidade direta subjetiva, o sêmen foi pré-diluído misturando-se 1 gota de sêmen + 4 gotas de solução aquosa não ativadora de NaCl a 2%.  A ativação dos espermatozóides foi efetuada mediante adição de 4 gotas de água destilada a uma gota da mistura, já ao microscópio pré-focalizado. As análises de motilidade, tanto do sêmen mantido à temperatura ambiente quanto do conservado sob resfriamento, foram realizadas a cada 1 hora. A motilidade do sêmen (Ŷ foi reduzindo-se, em função do tempo mantido à temperatura ambiente (X, segundo a equação: Ŷ= 93,2 – 13,0.X (P<0,01; R² ajustado = 81,7%. O sêmen resfriado apresentou motilidade constante num platô de 91,5% de motilidade por 4,4 horas, após o qual a motilidade (Ŷ caiu conforme transcorria o tempo sob resfriamento (X, de acordo com a equação: Ŷ = + 130,9 - 8,60.X (P<0,01; R² ajustado = 79,7%. Concluiu-se que o sêmen mantido sob resfriamento pode ser utilizado por até 11,7 horas.

  13. Vliv sádkovaní kapra na obsah a složení tuku

    OpenAIRE

    TRYBULOVSKÝ, Filip

    2014-01-01

    This bachelor's thesis deals with purging of common carp (Cyprinus carpio L.), and examines its effect on the content and composition of fatty acids and fat content. The goal of this experiment is to prove that purging has a positive effect on the carp flesh quality; that the progress and result of purging can be influenced by the input fish quality; and determining optimum time for carp purging with regard to the content and composition of fatty acids. The raising of fish was done at the pon...

  14. Aspects of embryonic and larval development in bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix

    Science.gov (United States)

    George, Amy E.; Chapman, Duane C.

    2013-01-01

    As bighead carp Hypophthalmichthys nobilis and silver carp H. molitrix (the bigheaded carps) are poised to enter the Laurentian Great Lakes and potentially damage the region’s economically important fishery, information on developmental rates and behaviors of carps is critical to assessing their ability to establish sustainable populations within the Great Lakes basin. In laboratory experiments, the embryonic and larval developmental rates, size, and behaviors of bigheaded carp were tracked at two temperature treatments, one “cold” and one “warm”. Developmental rates were computed using previously described stages of development and the cumulative thermal unit method. Both species have similar thermal requirements, with a minimum developmental temperature for embryonic stages of 12.1° C for silver carp and 12.9° C for bighead carp, and 13.3° C for silver carp larval stages and 13.4° C for bighead carp larval stages. Egg size differed among species and temperature treatments, as egg size was larger in bighead carp, and “warm" temperature treatments. The larvae started robust upwards vertical swimming immediately after hatching, interspersed with intervals of sinking. Vertical swimming tubes were used to measure water column distribution, and ascent and descent rates of vertically swimming fish. Water column distribution and ascent and descent rates changed with ontogeny. Water column distribution also showed some diel periodicity. Developmental rates, size, and behaviors contribute to the drift distance needed to fulfill the early life history requirements of bigheaded carps and can be used in conjunction with transport information to assess invasibility of a river.

  15. Carp Control on Montezuma National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — During the past few years the two pools at Montezuma National Wildlife Refuge have become heavily infested with Carp. These fish enter the pools over the tops of...

  16. Rapid establishment of pure lines of silver carp (Hypophthalmichthys molirix) and bighead carp (Aristichthys nobilis)

    Institute of Scientific and Technical Information of China (English)

    WANG Zhongwei; YE Yuzhen; ZHOU Jianfeng; WU Qingjiang

    2004-01-01

    The diversity of gynogenetic, artificial sex reversal and natural silver carp and bighead carp is examined using randomly amplified polymorphic DNA (RAPD) method.All of the 187 bands are obtained and 19 (10.16%) of them are polymorphic in gynogenetic silver carp.Meanwhile 32 (15.61%) out of 205 bands are polymorphic in control group.In gynogenetic bighead carp a total of 232 bands are identified and 11 (4.74%) out of them are polymorphic, while 25 (10.37%) out of 241 bands are polymorphic in control group.The genetic distance of four populations is calculated and it is 0.102 and 0.023 for gynogenetic silver carp and gynogenetic bighead carp respectively.The values of natural silver carp and bighead carp are 0.161 and 0.104.From the UPGMA trees constructed based on genetic distance, the sex reversal individuals that match with the gynogenetic female individuals are picked out.A new breeding process of establishing a pure line is developed.

  17. Carp Control on Montezuma National Wildlife Refuge 1954

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Carp Control in refuge pools has been an important project during the last three years on Montezuma National Wildlife Refuge. The necessity for curtailing the carp...

  18. Performance of orangefin labeo, Labeo calbasu (Hamilton 1822 as a component of polyculture system

    Directory of Open Access Journals (Sweden)

    Bibha Chetia Borah

    2014-04-01

    Full Text Available This paper deals with the performance of orangefin labeo, Labeo calbasu (Hamilton, 1822 as a component of conventional composite carp culture system. The species was included as 50% substitution to common carp (Cyprinus carpio in view of its omnivorous feeding habit and bottom dwelling nature. The total production was 0.55% higher in the experimental culture (with L. calbasu and Cyprinus carpio than in the control (common carp only. The difference in the production was however not significant statistically. The comparative economics revealed 5.30% higher return from the experimental unit. Comparison of growth pattern and survival rates of other five species of carps indicated that inclusion of L. calbasu did not have any adverse impact on the other carps. Growth rate of other carps was observed to be higher in the experimental set by 0.05% on an average than the control. Calculated profit per hectare was found to be 9.2% higher in experimental plot than in the control. Although the gross production and return in the experimental unit was not significantly higher than the control, the data supported that L. calbasu is compatible to other component species in composite carp culture system and its inclusion will enhance the economic viability of the system.

  19. Soybean β-Conglycinin Induces Inflammation and Oxidation and Causes Dysfunction of Intestinal Digestion and Absorption in Fish

    OpenAIRE

    Zhang, Jin-xiu; Guo, Lin-Ying; Feng, Lin; Jiang, Wei-Dan; Kuang, Sheng-Yao; Liu, Yang; Hu, Kai; Jiang, Jun; Li, Shu-Hong; Tang, Ling; Zhou, Xiao-Qiu

    2013-01-01

    β-conglycinin has been identified as one of the major feed allergens. However, studies of β-conglycinin on fish are scarce. This study investigated the effects of β-conglycinin on the growth, digestive and absorptive ability, inflammatory response, oxidative status and gene expression of juvenile Jian carp (Cyprinus carpio var. Jian) in vivo and their enterocytes in vitro. The results indicated that the specific growth rate (SGR), feed intake, and feed efficiency were reduced by β-conglycinin...

  20. Krmiva v rybářském sektoru jako zdroj eutrofizace povrchových vod

    OpenAIRE

    Halama, Zdeněk

    2014-01-01

    The work was designed to evaluate the quantity supplied of biogenic elements to the aquatic ecosystem by sports fishermen and the breeding and production of common carp Cyprinus carpio. The work focuses on the performance of essential nutrients, their cycle and the actual process of eutrophication with an overview of biogenic elements on which the work was focused, the effects of sport and breeding fish on water quality along with basic anatomy and overviews of the most common fish feed and b...

  1. Efficacy of sodium bicarbonate as anaesthetic for yellow seahorse, Hippocampus kuda (Bleeker, 1852)

    Digital Repository Service at National Institute of Oceanography (India)

    Pawar, H.B; Ingole, B; Sreepada, R.A.

    rapid recovery is better (Marking & Meyer, 1985; Stoskopf, 1993). An ideal anaesthetic should possess several attributes such Available online at http://www.urpjournals.com International Journal of Research in Fisheries and Aquaculture Universal... carp (Cyprinus carpio L., 1758) juveniles. Turk J Fish Aquat Sc. 9, 29-31. 2. Booke, H. E., Hollender, B. and Lutterbie, G. 1978. 3. Sodium bicarbonate, an inexpensive fish anesthetic for field use. Progressive Fish Culturist., 40, 11...

  2. Effective use RAS technolgy for breeding of ornamental fish in garden tanks.

    OpenAIRE

    MITÁŠ, Jaromír

    2014-01-01

    The aim of this thesis was to evaluate the efficiency of the technology RAS for water cleaning and conditioning for ornamental tanks with an emphasis on sustainable conservation of water transparency. Initial costs for building the tank, operational costs and costs of purchasing fish were compared. Results include measurements that have been carried out on private property of the author, who breeds ornamental koi carp (Cyprinus carpio). The filtration of breeding tank was formed by vortex, by...

  3. Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp

    Institute of Scientific and Technical Information of China (English)

    LIU ShaoJun; DUAN Wei; TAO Min; ZHANG Chun; SUN YuanDong; SHEN JiaMin; WANG Jing; LUO KaiKun; LIU Yun

    2007-01-01

    This study investigated the gynogenetic cytobiological behavior of the third gynogenetic generation (G3), which was generated from the diploid eggs produced by the second gynogenetic generation (G2)of red crucian carp × common carp, and determined the chromosomal numbers of G3, G2×scatter scale carp and G2×allotetraploid hybrids of red crucian carp × common carp. The results showed that the diploid eggs of G2 with 100 chromosomes, activated by UV-irradiated sperm from scatter scale carp and without the treatment for doubling the chromosomes, could develop into G3 with 100 chromosomes.Similar to the first and second gynogenetic generations (G1 and G2), G3 was also diploid (2n=100) and presented the hybrid traits. The triploids (3n=150) and tetraploids (4n=200) were produced by crossing G2 with scatter scale carp, and crossing G2 with allotetraploids, respectively. The extrusion of the second polar body in the eggs of G2 ruled out the possibility that the retention of the second polar body led to the formation of the diploid eggs. In addition, we discussed the mechanism of the formation of the diploid eggs generated by G2. The establishment of the diploid gynogenesis clonal line (G1, G2 and G3) provided the evidence that the diploid eggs were able to develop into a new diploid hybrid clonal line by gynogenesis. By producing the diploid eggs as a unique reproductive way, the diploid gynogenetic progeny of allotetrapioid hybrids of red crucian carp × common carp had important significances in both biological evolution and production application.

  4. MACROZOOBENTHOS IN CARP FISH FRY

    Directory of Open Access Journals (Sweden)

    Ljubica Debeljak

    1996-12-01

    Full Text Available Qualitative and quantitative components of macrozoobenthos was investigated on six fish farms with 8-10 ha-1 each at "Donji Miholjac". The ponds were stocked with 200,000 individuals per ha! of three-day-old carp larvae. The fry were fed with animal protein throughout the culturing season. The qualitative and quabtitative macrozoobenthos components formed mainly due to systematic groups of Oligochaeta and Chironomidae. The average size of Oligohaeta varied in each pond from 119 to 944 individuals per m-2 and from 2.18 to 14.09 g per m-2, and the larvae of Chironomidae from 174 to 1086 ind. per m-2 and from 2.66 to 14.09 g per m-2. Variation s of the total macrozoobenthos was within the amplitude of from 28 to 5805 ind. per m-2 and from 0.11 to 73.72 g. per m-2. In mid summer it was determined that there was a fall in the amount of macrozoobenthos in all fish ponds (Table 6.

  5. Probiotics in carp fish nutrition

    Directory of Open Access Journals (Sweden)

    Ljubojević Dragana

    2013-01-01

    Full Text Available Uncontrolled use of antibiotics in aquaculture caused the appearance and spreading of pathogens resistance to antibiotics, what consequently drove up to the necesity of finding new technologies for protecting aquatic organisms from pathogens. Probiotics are aimed to reduce antibiotics application, and furthermore they have an important role not only in disease prevention but also in efficiency of food utilization and improvement of production parameters. Carp fish species are economically most significant in Republic of Serbia, therefore this work summarizes the results of previous studies of antibiotics application in this kind in particular. There is also pointed out to numerous harmful side effects of antibiotics use in aquaculture, and described the previous results of investigations on mechanism of probiotics effects and specificity of their use in this field as well. Beside this, there are summarized the results that show positive influence of probiotics in cyprinides nutrition on production performance, haematological parameters, course of experimental infection, activity of digestive enzymes. Special attention is paid to criteria for proper selection of probiotics in cyprinides production. [Projekat Ministarstva nauke Republike Srbije, br. TP 31011: Uticaj kvaliteta komponenata u ishrani ciprinida na kvalitet mesa, gubitke i ekonomičnost proizvodnje

  6. The Influence of Probiotics on Results of Common carp,Crucian Carp,and Grass Carp Culture%微生态制剂对鲤、鲫和草鱼养殖池塘效益的影响

    Institute of Scientific and Technical Information of China (English)

    李绍戊; 王荻; 尹家胜; 卢彤岩

    2011-01-01

    The effects of probiotics I containing primarily Bacillus subtilis and probiotics II containing a mixture of various microorganisms on weight gain,food conversion ratio and drug cost of common carp(Cyprinus carpio),Crucian carp(Carassius auratus) and grass carp(Ctenophyargodon idellus) in culture ponds in Heilongjiang and Liaoning provinces.The probiotics,alone or together,were showed to have influence on weight gain,the food conversion ratio and the drug cost in the fish culture.There were 423.57% higher weight gain in Heilongjiang province and 90% higher weight gain in Liaoning province in the ponds treated with the probiotics used together,than that in the control ponds with lower drug production cost of 29% in Heilongjiang province and 56.25% in Liaoning province.The food conversion ratio was found to be decreased by 0.35 and 0.05 in the ponds treated with probiotics used together,indicating that the probiotics have important influence on the profit of grass carp culture in Liaoning province and of common carp farming in Heilongjiang and have a promotion of green aquaculture with less drugs,less feed and more profits.%以枯草芽孢杆菌为主的主要用于促进鱼类消化生长的微生态制剂I、多种混合微生物主要用于调节水质的微生态制剂II,或是两者混合使用的方法,比较研究了黑龙江省及辽宁省池塘养殖的鲤(Cyprinus carpio)、鲫(Carassius auratus)和草鱼(Ctenophyargodon idellus)的增重率、饲料系数及药价等效益。结果表明,单种或者混合使用微生态制剂均对池塘养殖鲤及草鱼的增重率、饵料系数及用药价格有一定影响,同时使用两种微生态制剂提高鱼类增重率最显著,辽宁省和黑龙江省分别提高了423.57%和90%;而两省养殖池塘的药价也分别降低了29%和56.25%。微生态制剂I及混合同时使用两种微生态制剂均能显著降低饲料系数,辽宁及黑龙江分别降低0.35及0.05。结果可

  7. Population Structure Analysis of Common Carp of Yangtze River and Heilongjiang River Using EST-SSRs Markers%用鲤鱼EST-SSRs分子标记分析长江黑龙江鲤种群结构

    Institute of Scientific and Technical Information of China (English)

    鲁翠云; 全迎春; 李大宇; 孙效文; 梁利群

    2007-01-01

    从鲤鱼(Cyprinus carpio)ESTs的数据库(10 691个)中进行微卫星序列筛选,共发现微卫星序列127个,占整个ESTs数据库的1.19%;另外从本研究室构建的鲤鱼脑组织cDNA文库中筛选到微卫星序列20个.根据筛选得到的微卫星序列设计引物68对,合成引物40对,其中27对引物能够获得预期的目的片段,20对引物在所检测的群体内及群体间表现出多态性.用此20对引物标记的多态性分析了长江鲤(Cyprinus carpio)和黑龙江鲤(C. carpio var haematopaerus.Temminck et Schlegel)的群体遗传结构,结果表明:长江鲤和黑龙江鲤在多数位点表现出较高的遗传多样性,20个位点的平均有效等位基因数分别为3.9135和3.4820;平均观察杂合度为0.6067和0.5667;平均期望杂合度为0.6737和0.6194;平均多态信息含量为0.6308和0.5714.长江鲤的遗传结构好于黑龙江鲤,两个群体的Hardy-Weinberg偏离指数较小,群体基本保持平衡,群体结构较合理;其中HLJE1和HLJE10位点在长江鲤和黑龙江鲤的扩增结果相差较大,长江鲤的多态性明显高于黑龙江鲤,位点多态信息含量相差1倍以上,可用于群体的鉴别.

  8. Bigheaded carps : a biological synopsis and environmental risk assessment

    Science.gov (United States)

    Kolar, Cindy S.; Chapman, Duane C.; Courtenay, Walter R., Jr.; Housel, Christine M.; Williams, James D.; Jennings, Dawn P.

    2007-01-01

    The book is a detailed risk assessment and biological synopsis of the bigheaded carps of the genus Hypophthalmichthys, which includes the bighead, silver, and largescale silver carps. It summarizes the scientific literature describing their biology, ecology, uses, ecological effects, and risks to the environment.

  9. A Study of the Carpe Diem in Two Literary Poems

    Institute of Scientific and Technical Information of China (English)

    万彰劼

    2011-01-01

    Carpe diem expresses a philosophy of recognizing the brevity of life and seizing an opportunity in time.Poems then breathe wit and wisdom with the carpe diem theme running through,on the basis of which many distinguished Cavalier poets have created a numb

  10. Carp's Tongue Swords in Spain, France and Italy

    OpenAIRE

    Hugh O'Neill HENCKEN

    2009-01-01

    Professor Almagro in his article on the Huelva hoard has compared swords with carp's tongue points from that find to the well-known sword from Veii. In this connection some comment on carp's tongue points in Italy and their possible connection with the west may be of interest.

  11. Asian carp behavior in response to static water gun firing

    Science.gov (United States)

    Layhee, Megan J.; Gross, Jackson A.; Parsley, Michael J.; Romine, Jason G.; Glover, David C.; Suski, Cory D.; Wagner, Tristany L.; Sepulveda, Adam J.; Gresswell, Robert E.

    2013-01-01

    The potential for invasion of Asian carp into the Great Lakes has ecological and socio-economic implications. If they become established, Asian carp are predicted to alter lake ecosystems and impact commercial and recreational fisheries. The Chicago Sanitary and Shipping Canal is an important biological conduit between the Mississippi River Basin, where invasive Asian carp are abundant, and the Great Lakes. Millions of dollars have been spent to erect an electric barrier defense in the canal to prevent movement of Asian carp into the Great Lakes, but the need for additional fish deterrent technologies to supplement the existing barrier is warranted. Scientists with the U.S. Geological Survey Northern Rocky Mountain Science Center are examining seismic water gun technology, formerly used in oceanic oil exploration, as a fish deterrent. The goal of the current study is to employ telemetry and sonar monitoring equipment to assess the behavioral response of Asian carp to seismic water guns and the sound energy it generates.

  12. Determination of the acute toxicity of isoniazid to three invasive carp species and rainbow trout in static exposures

    Science.gov (United States)

    Schreier, Theresa M.; Hubert, Terrance D.

    2015-01-01

    Three invasive fishes of considerable concern to aquatic resource managers are the Hypophthalmichthys nobilis (bighead carp),Hypophthalmichthys molitrix (silver carp), and Ctenopharyngodon idella (grass carp), collectively known as Asian carps. There is a need for an effective chemical control agent for Asian carps. Isoniazid was identified as a potential toxicant for grass carp. The selective toxicity of isoniazid to grass carp was verified as a response to an anecdotal report received in 2013. In addition, the toxicity of isoniazid to bighead carp, silver carp, and Oncorhynchus mykiss (rainbow trout) was evaluated. Isoniazid was not toxic to grass carp at the reported anecdotal concentration, which was 13 milligrams per liter. Isoniazid (130 milligrams per liter) was not selectively toxic to bighead carp, silver carp, or grass carp when compared to rainbow trout.

  13. Comparative studies on in vitro sperm decondensation and pronucleus formation in egg extracts between gynogenetic and bisexual fish

    Institute of Scientific and Technical Information of China (English)

    CHANG JIAN LI; JIAN FANG GUI

    2003-01-01

    A cell-free system based upon the egg extracts from gynogenetic gibel carp (Carassius auratus gibelio)or bisexual red common carp (Cyprinus carpio red variety) was developed to investigate developmentalbehaviors of the demembranated sperm nuclei. Both red common carp and gibel carp sperm nuclei coulddecondense fully and form pronuclei in the red common carp egg extracts. Gibel carp sperm nuclei couldalso decondense fully and form pronuclei in the gibel carp egg extracts, but red common carp sperm nucleicould not decondense sufficiently in the same extracts. The significant differences of morphological changeswere further confirmed by ultrastructural observation of transmission electron microscopy. The data furtheroffer cytological evidence for gonochoristic reproduction in the gynogenetically reproducing gibel carp. Inaddition, the sperm nuclei in vitro decondensation is dependent on the pH in the extracts, and the decon-densed efficiency is optimal at pH 7. However, no DNA replication was observed in the two kinds of eggextracts during the incubation period of the sperm nuclei. It is suggested that the egg extracts preparedfrom the gynogenetic gibel carp should be a valid in vitro system for studying molecular mechanism ongynogenesis and reproduction mode diversity in fish.

  14. Molecular responses differ between sensitive silver carp and tolerant bighead carp and bigmouth buffalo exposed to rotenone

    Science.gov (United States)

    Amberg, Jon J.; Schreier, Theresa M.; Gaikowski, Mark P.

    2012-01-01

    Some species of fish are more tolerant of rotenone, a commonly used non-specific piscicide, than others. This species-specific tolerance to rotenone has been thought to be associated with the uptake and the efficiency at which the chemical is detoxified. However, rotenone stimulates oxidative stress and superoxides, which are also toxic. Understanding the modes in which fish physiologically respond to rotenone is important in developing improved protocols for its application in controlling aquatic nuisance species. Using a molecular approach, we investigated the physiological and molecular mechanisms of rotenone resistance. Species-specific responses were observed when rotenone-sensitive silver, Hypophthalmichthys molitrix, and both rotenone-resistant bighead carp, Hypophthalmichthys nobilis, and bigmouth buffalo, Ictiobus cyprinellus, were exposed to rotenone. Rotenone levels in plasma were highest 90 min after exposure in both silver carp and bigmouth buffalo, but bigmouth buffalo tolerated over twice the burden (ng mL-1 g-1) than silver carp. Expression of genes related with detoxification (cyp1a and gst) increased in silver carp, but either decreased or remained the same in bighead carp. Genes linked with oxidative stress in the cytosol (gpx, cat and sod1) and hsp70 increased only in silver carp after a 6-h exposure. Expression of genes associated with oxidative stress in the mitochondria (sod2 and ucp2) differed between silver carp and bighead carp. Expression of sod2 changed minimally in bighead carp, but expression of ucp2 linearly increased to nearly 85-fold of the level prior to exposure. Expression of sod2 and ucp2 did not change until 6 h in silver carp. Use of sod1 and sod2 to combat oxidative stress results in hydrogen peroxide production, while use of ucp2 produces nitric oxide, a chemical known to inhibit apoptosis. We conclude that the mechanism at which a fish handles oxidative stress plays an important role in the tolerance to rotenone.

  15. Activity Inducement of Oil Wastewater on AHH of Cypinus Carpio Hepatic Microsome%含油废水对鲤鱼肝脏微粒体芳烃羟化酶的活性诱导研究

    Institute of Scientific and Technical Information of China (English)

    邵涛

    2012-01-01

    The aryl hydrocarbon hydroxylase(AHH) activities of cyprinus carpio liver microsome induced by three oil wastewaters have been examined in Vitro.It was found that all the tested chemicals show different degrees of inducement on AHH activity.The AHH activity becomes higher with the increase of oil quantity.The AHH activity of fish liver microsome can be used as a biomarker to evaluate the oil wastewater in waters.%以鲤鱼肝脏微粒体为实验体系,运用体外实验方法,研究三种含油废水对芳烃羟化酶的活性影响,结果表明,三种含油废水对芳烃羟化酶活性都表现出不同程度的诱导,随着含油量的增大,AHH活性升高,并有相似的变化趋势。芳烃羟化酶活性升高可以作为监测含油废水对水体污染的一种生物指标。

  16. MERCURY INTOXICATION IN GRASS CARP (CTENOPHARYNGODON IDELLA)

    OpenAIRE

    M. S. Khan, S. A. Khan, Z. I. Chaudhary, M. N. Khan,1 A. Aslam , K. Ashraf2, R. M. Ayyub and M. F. Rai.

    2004-01-01

    The present project was carried out to study the effects of acute and chronic mercury intoxication in Grass carp (Ctenopharyngodon idella). For acute phase experiment, 48 fish were divided into four equal groups (A, B, C and D). Groups B, C and D were given HgCl2 at sublethal dose as 0.4, 0.5 and 0.6 mg/L, respectively, while group A acted as control. Skin, gills and kidneys were isolated from the fish after 48 and 96 hours for pathological studies. For chronic phase, 72 fish were divided int...

  17. Triple focussing recoil separator CARP at RCNP

    International Nuclear Information System (INIS)

    A reaction product mass separator (CARP) which is now being constructed for use with the AVF cyclotron at RCNP is described. This device is intended to separate unslowed recoiling products in nuclear reactions from the primary beam and to analyze them according to their charge-to-mass ratio. The use as a mass-spectrograph or as a mass-separator is available according to the experimental requirements. The solid angle and the energy range of acceptance will be 10 msr and 20%, respectively. (orig.)

  18. Body color development and genetic analysis of hybrid transparent crucian carp (Carassius auratus).

    Science.gov (United States)

    Xu, W; Tong, G X; Geng, L W; Jiang, H F

    2015-01-01

    The aim of this study was to investigate the genetic mechanism of the transparent trait in transparent crucian carp. We observed body color development in transparent crucian carp larvae and analyzed heredity of color in hybrids produced with red crucian carp, ornamental carp, and red purse carp. The results showed that the body color of the newly hatched larvae matured into the adult pattern at approximately 54 days post-hatching. Two inter-species reciprocal crosses between transparent crucian carp and red crucian carp, and self-cross F1 of transparent crucian carp and self-cross F1 of red marking transparent crucian carp were conducted, and results indicated that the transparent-scaled trait is dominant over the normal-scaled trait. Furthermore, the transparent trait is a quantitative trait. All offspring in the four inter-genera reciprocal crosses of transparent crucian carp with ornamental carp and red purse carp were hybrids of common carp and crucian carp, and had a relatively low survival rate of 10-20%. Moreover, the transparent-scaled trait was observed to be dominant over the normal-scaled trait in the hybrid fish. In conclusion, our results suggest that the genetic mechanism underlying the color of goldfish is complex and requires further investigation. PMID:25966213

  19. Meiobenthos provides a food resource for young cyprinids.

    Science.gov (United States)

    Spieth, H R; Möller, T; Ptatscheck, Ch; Kazemi-Dinan, A; Traunspurger, W

    2011-01-01

    Young individuals of the bottom-biting (i.e. sediment-ingesting) common carp Cyprinus carpio and gudgeon Gobio gobio consumed significant amounts of nematodes in laboratory experiments, whereas the selective-feeding roach Rutilus rutilus did not. In mesocosm enclosure experiments in the field, C. carpio strongly decreased the nematode abundance within 4 days, whereas the bottom-biting bream Abramis brama did not affect the abundance until after 14 days. In controlled experiments with a known number of prey, C. carpio but not A. brama significantly reduced the number of nematodes, and G. gobio reduced the nematode abundance dependent on the size of the fish, with smaller fish causing a greater reduction. Cyprinus carpio consumed the nematodes and did not just mechanically kill them in the sediment, as shown by dissection of the fish intestine. Morphometric analysis of the branchial baskets indicated that the mesh width of C. carpio, but not of A. brama, is suitable for consuming meiobenthos. The results indicate that the meiobenthos is a food resource for certain bottom-feeding freshwater fishes. PMID:21235551

  20. Malheur - Efficacy of Electrofishing to Reduce Recruitment of Common Carp

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Our goal is to investigate the efficacy of using a portable electrofishingsystem to help control common carp by killing their eggs and embryos at Malheur...

  1. Time to let go of CARP? Not so fast

    OpenAIRE

    Toby C. Monsod; Sharon A. Piza

    2014-01-01

    The following note seeks to clarify the appreciation of data pertaining to agrarian reform as used in the discussion paper "CARP: time to let go" (henceforth Fabella (2014)). Fabella (2014) has three parts: the first part argues that "the cumulative weight of evidence suggests that the hypothesis that in economic terms CARP is a government failure has not been rejected." The second part offers possible reasons for that failure. The third part concludes, saying it is time to let go.

  2. Predator-induced morphology enhances escape locomotion in crucian carp

    OpenAIRE

    Domenici, Paolo; Turesson, Håkan; Brodersen, Jakob; Brönmark, Christer

    2007-01-01

    Fishes show a remarkable diversity of shapes which have been associated with their swimming abilities and anti-predator adaptations. The crucian carp (Carassius carassius) provides an extreme example of phenotypic plasticity in body shape which makes it a unique model organism for evaluating the relationship between body form and function in fishes. In crucian carp, a deep body is induced by the presence of pike (Esox lucius), and this results in lower vulnerability to gape-limited predators,...

  3. Estudio citoquímico del epitelio branquial de "Cyprinus carpio" su participación en los procesos de osmorregulación y permeabilidad

    OpenAIRE

    Amores Ferreras, Petra

    1989-01-01

    La branquia de los peces teleósteos es uno de los órganos que, anatómica e histológicamente, ha alcanzado un mayor grado de diferenciación a lo largo de la escala zoológica. Tal hecho está basado en su multifuncionalidad ya que, junto con la respiración,

  4. The effect of hybridization on fish physiology, immunity and blood biochemistry: A case study in hybridizing Cyprinus carpio and Carassius gibelio (Cyprinidae)

    Czech Academy of Sciences Publication Activity Database

    Šimková, A.; Vojtek, L.; Halačka, Karel; Hyršl, P.; Vetešník, Lukáš

    2015-01-01

    Roč. 435, č. 1 (2015), s. 381-389. ISSN 0044-8486 R&D Projects: GA ČR GAP505/12/0375 Institutional support: RVO:68081766 Keywords : Hybridization * Cyprinids * Blood biochemistry * Hematology * Immunity Subject RIV: EG - Zoology Impact factor: 1.878, year: 2014

  5. The effect of hybridization on fish physiology, immunity and blood biochemistry: a case study in hybridizing Cyprinus carpio and Carassius gibelio

    Czech Academy of Sciences Publication Activity Database

    Vetešník, Lukáš; Halačka, Karel; Vojtek, L.; Hyršl, P.; Šimková, A.

    Prešov: Vydavateľstvo Prešovskej univerzity, 2014 - (Manko, P.; Baranová, B.). s. 227 ISBN 978-80-555-1140-5. [Zoológia 2014, Feriancove dni /19./. 20.11.2014-22.11.2014, Prešov] R&D Projects: GA ČR GAP505/12/0375 Institutional support: RVO:68081766 Keywords : cyprinids * hybridization * haematology * blood chemistry * immunity and metazoan parasites Subject RIV: EG - Zoology

  6. Differential expression of two C-type lectins in grass carp Ctenopharyngodon idella and their response to grass carp reovirus.

    Science.gov (United States)

    Ju, C S; He, L B; Pei, Y Y; Jiang, Y; Huang, R; Li, Y M; Liao, L J; Jang, S H; Wang, Y P

    2016-02-01

    The cDNAs of two C-type lectins in grass carp Ctenopharyngodon idella, galactose-binding lectin (galbl) and mannose-binding lectin (mbl), were cloned and analysed in this study. Both of them exhibited the highest expression level in liver, whereas their expression pattern differed in early phase of embryonic development. Following exposure to grass carp reovirus (GCRV), the mRNA expression level of galbl and mbl was significantly up-regulated in liver and intestine. PMID:26643267

  7. Carp Control Project in the Main Pool at Montezuma National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Over the past several years carp have become an increasing problem in the management of the Montezuma National Wildlife Refuge for Waterfowl. Carp compete directly...

  8. VARIOUS FACTORS AFFECTING DRESSING PERCENTAGE OF COMMERCIALLY CULTURED CYPRINID FISH IN CARP FISH PONDS IN SERBIA

    Directory of Open Access Journals (Sweden)

    Todor Marković

    2012-12-01

    Full Text Available The aim of this study was to determine the yield carcass of all categories of cyprinid fish reared in ponds in Serbia. Samples of two and three-year old carp, two-year old silver carp and grass carp were taken in the winter from a pond where the production is organized in a semi­intensive system. The three­year old carp was sampled from two ponds. In one case, it fed on barley, maize and wheat in the following proportions 40:30:30, while in the second case it fed on complete diet mixtures. Also, the samples of two­year old carp were taken from ponds where they fed on complete feed mixture. Dressing percentage was the most favourable in common carp (67%, followed by silver carp (62%, and it was the least in grass carp (60% (p<0.01. The best yield (66% was obtained in two-year old carp, followed by one-year old carp (64%, and the worst yield was determined in three-year old carp (58% (p<0.01. Carcasses yield was better in two-year old carp fed on pelleted feed (68% than in carp of the same age fed on grains (66% (p<0.01. The values of dressing percentage measured in three-year old carp reared in the semi-intensive system was 56%, and 59% in three-year old carp fed on pelleted complete feed mixture. Fish species, age, system of husbandry and diet showed a significant effect on carcasses yield. The highest dressing percentage and weight of fillets was noted in two-year old carp fed on complete feed, and it was a result of lower weight of internal organs and associated fat. The obtained results may be helpful in creating the best strategy for the selection of raw fish for fish manufacturing.

  9. Detection of grass carp reovirus (GCRV) with monoclonal antibodies.

    Science.gov (United States)

    Hongli, Jing; Lifeng, Zhang; Zhenzhen, Fang; Lipu, Xu; Min, Zhang; Na, Wang; Yulin, Jiang; Xiangmei, Lin

    2014-04-01

    Grass carp reovirus (GCRV) is a pathogen that causes hemorrhagic disease of grass carp. It is the most serious infectious disease of carp and causes serious losses of fingerlings of grass carp and black carp. In this study, a recombinant VP4, one of the viral core proteins, was constructed with a histidine tag and expressed at a high level in E. coli, and the expressed protein was mainly found in the form of inclusion bodies. The expressed VP4 protein was recognized by an anti-His-tag monoclonal antibody and goat anti-GCRV serum. Four monoclonal antibodies (16B7, 39E12, 13C3 and 14D1) against the recombinant VP4 protein were produced. These MAbs did not react with any of the tested viruses or fish cells lines in the ELISA tests except GCRV. In western blotting analysis, a protein band was observed when the recombinant VP4 protein of GCRV was used as an antigen, but a 68-kDa band was observed when natural capsid proteins of GCRV were used as antigens. Furthermore, a sandwich ELISA was developed for detection of GCRV. The detection limit of the test was 105 TCID50 of GCRV per mL. PMID:24122108

  10. Culture potential of Amblypharyngodon mola with carps in polyculture in farmers' pond of northern regions of Bangladesh

    OpenAIRE

    Kunda, M.; Nahid, S.A.A.; Wahab, M. A.; Thilsted, S.H.; Roos, N.

    2009-01-01

    To assess the culture potential of mola (Amblypharyngodon mola) along with carps in polyculture systems, an experiment consisted of three treatments each with five replications was conducted for 4 months in two villages of Parbatipur upazilla under Dinajpur district. In the first treatment (SS), catla, rohu, mrigal, grass carp, Thai punti, common carp and a higher density of silver carp (8 per 40m²) were stocked. In the second treatment (SM), stocking density of silver carp was reduced to ...

  11. Content comparison of polycyclic aromatic hydrocarbons (PAHs in traditionally smoked freshwater fish

    Directory of Open Access Journals (Sweden)

    Renata Pietrzak-Fiećko

    2015-09-01

    Full Text Available The aim of this study was to compare the content of chosen PAHs (benzo[a]pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene in meat of different freshwater fish species that were traditionally (directly smoked. The survey covered 16 freshwater fish samples of four species: rainbow trout (Oncorhynchus mykiss, carp (Cyprinus carpio, vendace (Coregonus albula and eel (Anguilla anguilla. Average content of sum of four PAHs in meat of trout, carp, vendace and eel was: 2.73, 8.23, 6.45 and 3.65 µg/kg of meat, respectively. Maximum levels of chosen PAHs in meat of examined fish species were not exceeded by any of the samples. Conducted studies confirmed that meat of traditionally smoked trout, carp, vendace and eel is safe in terms of content of the analysed PAHs.

  12. Polychlorinated Biphenyls (PCBs) in Catfish and Carp Collected from the Rio Grande Upstream and Downstream of Los Alamos National Laboratory: Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Gilbert J. Gonzales

    2008-05-12

    Concern has existed for years that the Los Alamos National Laboratory (LANL), a complex of nuclear weapons research and support facilities, has released polychlorinated biphenyls (PCBs) to the environment that may have reached adjacent bodies of water through canyons that connect them. In 1997, LANL's Ecology Group began measuring PCBs in fish in the Rio Grande upstream and downstream of ephemeral streams that cross LANL and later began sampling fish in Abiquiu and Cochiti reservoirs, which are situated on the Rio Chama and Rio Grande upstream and downstream of LANL, respectively. In 2002, we electroshocked channel catfish (Ictalurus punctatus) and common carp (Carpiodes carpio) in the Rio Grande upstream and downstream of LANL and analyzed fillets for PCB congeners. We also sampled soils along the Rio Chama and Rio Grande drainages to discern whether a background atmospheric source of PCBs that could impact surface water adjacent to LANL might exist. Trace concentrations of PCBs measured in soil (mean = 4.7E-05 {micro}g/g-ww) appear to be from background global atmospheric sources, at least in part, because the bimodal distribution of low-chlorinated PCB congeners and mid-chlorinated PCB congeners in the soil samples is interpreted to be typical of volatilized PCB congeners that are found in the atmosphere and dust from global fallout. Upstream catfish (n = 5) contained statistically (P = 0.047) higher concentrations of total PCBs (mean = 2.80E-02 {micro}g/g-ww) than downstream catfish (n = 10) (mean = 1.50E-02 {micro}g/g-ww). Similarly, upstream carp (n = 4) contained higher concentrations of total PCBs (mean = 7.98E-02 {micro}g/g-ww) than downstream carp (n = 4) (3.07E-02 {micro}g/g-ww); however, the difference was not statistically significant (P = 0.42). The dominant PCB homologue in all fish samples was hexachlorobiphenyls. Total PCB concentrations in fish in 2002 are lower than 1997; however, differences in analytical methods and other uncertainties

  13. Case report of chondroma in a grass carp (Ctenopharyngodon idella)

    Science.gov (United States)

    Mesbah, Mehrzad; Rezaie, Annahita; Tulaby Dezfuly, Zahra

    2016-01-01

    The grass carp (Ctenopharyngodon idella) is a herbivorous, freshwater fish species of the family Cyprinidae, and the only species of the genus Ctenopharyngodon. Neoplasms in fishes are generally less aggressive than neoplasms in mammals and are most commonly discrete, focal and benign neoplasms. A 3-year-old grass carp with a big mass on the vertebrae was referred to the clinic. According to the owner’s statements, the fish had no signs of lethargy, ataxia and abnormal behaviors. The size of the mass was 7 × 6 × 6 cm. It cut hardly with audible sounds. The consistency of the mass was as hard as a cartilage. Microscopic examination revealed numerous irregular crests of hyaline cartilage beneath the skin. According to histopathologic characteristics, chondroma on the vertebrae of grass carp was diagnosed.

  14. Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes

    Directory of Open Access Journals (Sweden)

    Takahiko Aoki

    2014-08-01

    Full Text Available We isolated a high-purity carp glycophorin from carp erythrocyte membranes following extraction using the lithium diiodosalicylate (LIS-phenol method and streptomycin treatment. The main carp glycophorin was observed to locate at the position of the carp and human band-3 proteins on an SDS-polyacrylamide gel. Only the N-glycolylneuraminic acid (NeuGc form of sialic acid was detected in the carp glycophorin. The oligosaccharide fraction was separated into two components (P-1 and P-2 using a Glyco-Pak DEAE column. We observed bacteriostatic activity against five strains of bacteria, including two known fish pathogens. Fractions from the carp erythrocyte membrane, the glycophorin oligosaccharide and the P-1 also exhibited bacteriostatic activity; whereas the glycolipid fraction and the glycophorin fraction without sialic acid did not show the activity. The carp glycophorin molecules attach to the flagellum of V. anguillarum or the cell surface of M. luteus and inhibited bacterial growth.

  15. Treatment of fishpond water by recirculating horizontal and vertical flow constructed wetlands in the tropics

    DEFF Research Database (Denmark)

    Konnerup, Dennis; Trang, Ngo Thuy Diem; Brix, Hans

    2011-01-01

    in the outlets. The ornamental Canna×generalis planted in the CWs grew faster and took up more N and P in the vertical flow CWs. The aquaculture fish had a feed conversion ratio of 1.53 based on feed dry weight, and 31% and 34% of N and P input, respectively, were incorporated into fish biomass. Only minor...... niloticus) and common carp (Cyprinus carpio) was recirculated through horizontal and vertical flow CWs. The CWs were able to keep a good water quality with DO (>1mg/l-1), BOD (fish. There was a good removal...

  16. Influence of the geothermal waters on fishes and aquatic invertebrates, in the Plain of Banat

    Energy Technology Data Exchange (ETDEWEB)

    Popescu-Marinescu, V.; Elain-Talau, L.; Papadopol, M.; Nicolescu, D.; Teodorescu, L.

    1982-01-01

    Research on the effects of geothermal waters (both used and unused) on vertebrates and invertebrates made the water's toxico-lethal character obvious in waters with a normal concentration or diluted up to over 17%. Among the organisms used in the experiment, Daphnia magna (a cladoceran) and young carp (Cyprinus carpio) have proved to be more sensitive. Tubifex tubifex (an oligochaete) have proved to be more resistant. Taking in account the toxicity of geothermal waters with addition of a specific smell and taste contracted by the meat fish, their overflow into natural receivers is not recommended. 7 references, 12 figures.

  17. Functional morphology of durophagy in black carp, Mylopharyngodon piceus.

    Science.gov (United States)

    Gidmark, Nicholas J; Taylor, Chantel; LoPresti, Eric; Brainerd, Elizabeth

    2015-12-01

    The black carp, Mylopharyngodon piceus (Osteichthyes: Cyprinidae), crushes its snail and other molluscan prey with robust pharyngeal jaws and strong bite forces. Using gross morphology, histological sectioning, and X-ray reconstruction of moving morphology (XROMM), we investigated structural, behavioral, and mechanical aspects of pharyngeal jaw function in black carp. Strut-like trabeculae in their pharyngeal jaws support large, molariform teeth. The teeth occlude with a hypertrophied basioccipital process that is also reinforced with stout trabeculae. A keratinous chewing pad is firmly connected to the basioccipital process by a series of small bony projections from the base of the pedestal. The pharyngeal jaws have no bony articulations with the skull, and their position is controlled by five paired muscles and one unpaired median muscle. Black carp can crush large molluscs, so we used XROMM to compare pharyngeal jaw postures as fish crushed ceramic tubes of increasing sizes. We found that black carp increase pharyngeal jaw gape primarily by ventral translation of the jaws, with ventral rotation and lateral flaring of the jaws also increasing the space available to accommodate large prey items. A stout, robust ligament connects left and right jaws together firmly, but allows some rotation of the jaws relative to each other. Contrasting with the pharyngeal jaw mechanism of durophagous perciforms with fused left and right lower pharyngeal jaws, we hypothesize that this ligamentous connection may serve to decouple tensile and compressive forces, with the tensile forces borne by the ligament and the compressive forces transferred to the prey. PMID:26289832

  18. Biological characteristics of an improved triploid crucian carp

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    An improved triploid crucian carp(ITCC) was produced by crossing improved tetraploids(G1×AT,) with improved red crucian carp(IRCC,),which were obtained by distant crossing and gynogenesis.The biological characteristics of ITCC,including the number and karyotype of chromosomes,gonadad and pituitary structures,phenotype,and growth rate are reported.ITCC possessed 150 chromosomes with the karyotype 33m+51sm+33st+33t.In the breeding season,both ovary-like and testis-like gonads of ITCC were unable to produce normal mature gametes.The ultrastructure of the pituitary of ITCC showed that most of the endocrine granules in gonadotrophic hormone(GTH) cells had not been released,providing endocrinological evidence for the sterility of ITCC.Compared with triploid crucian carp(TCC) produced by mating Japanese crucian carp with allotetraploid hybrids,ITCC not only retained the excellent traits of fast growth rate and sterility,but also acquired improved morphological characteristics,including higher body,shorter tail and smaller head.

  19. Culicidofauna Asociada con Contenedores Artificiales en la Comunidad "La Carpio", Costa Rica Culicidfauna associated to artificial containers in the neighborhood "La Carpio", Costa Rica

    OpenAIRE

    Olger Calderón Arguedas; Adriana Troyo; Solano, Mayra E.; Adrián Avendaño

    2009-01-01

    Objetivo: Determinar las especies de mosquitos más frecuentes en "La Carpio", primer comunidad del Área Metropolitana de San José en sufrir un brote por dengue. Métodos: Cuatro encuestas larvales fueron realizadas. La evaluación incluyó el análisis de todos los contenedores intradomiciliares y peridomiciliares que acumularon agua. Las muestras larvales fueron fijadas en alcohol al 70 %, aclaradas en lactofenol y montadas en medio Hoyer para su observación microscópica. La infestación fue expr...

  20. Grass carp Ctenopharyngodon idella Fibulin-4 as a potential interacting partner for grass carp reovirus outer capsid proteins.

    Science.gov (United States)

    Yu, Fei; Wang, Hao; Liu, Weisha; Lu, Liqun

    2016-01-01

    Mammalian EGF containing fibulin-like extracellular matrix protein 2 (Fibulin-4/EFEMP2), an extracellular matrix(ECM) protein and a member of the fibulin family, is involved in elastic fiber formation, connective tissue development and some human diseases. In a yeast-two hybrid screening of host proteins interacting with outer capsid protein of grass carp reovirus (GCRV), a grass carp homologue of Fibulin-4 (designated as GcFibulin-4) is suggested to hold the potential to bind VP7, VP56 and VP55, the outer capsid protein encoded by type I, II, III GCRV, respectively. GcFibulin-4 gene of grass carp was cloned and sequenced from the cDNA library constructed for the yeast two-hybrid screening. Full-length cDNA of GcFibulin-4 contains an open reading frame (ORF) of 1323 bp encoding a putative protein of 440 amino acids. Phylogenetic analysis of GcFibulin-4 indicated that it shared a high homology with zebra fish Fibulin-4 protein. Transcriptional distribution analysis of GcFibulin-4 in various tissues of healthy grass carp showed that GcFibulin-4 was highly expressed in muscle, moderately expressed in the intestine and brain, and slightly expressed in other examined tissues; the expression pattern is consistent with tissue tropism of GCRV resulting in hemorrhage symptom in the corresponding tissues. Our results suggested that Fibulin-4 might enable free GCRV particles, the pathogen for grass carp hemorrhagic disease, to target fish tissues more efficiently by interacting with viral outer capsid proteins. PMID:26626583

  1. Studies on the suitability of producing value-added products from silver carp, Hypophthalmichthys molitrix

    OpenAIRE

    Alam, A.K.M.N.

    2005-01-01

    Silver carp, Hypophthalmichthys molitrix is contributing significantly to the total production of fish through aquaculture in Bangladesh. However, its low market price has become a serious concern to the fish farmers. The suitability of silver carp mince for the production of various value-added products (VAPs) - surimi, fish sausage, fish burger and fish stick was studied during April-September 2000 to ensure more appropriate and profitable utilization of silver carp. Surimi/frozen mince blo...

  2. Development of supplemental diets for carp in Vietnamese upland ponds based on locally available resources

    OpenAIRE

    Nguyen Ngoc, Tuan

    2010-01-01

    Cyprinids play very important role in aquaculture, especially in Asia and Pacific regions. Vietnam is not an exception. Although aquaculture in Vietnam increased dramatically recently, its high value products such as catfish, tiger prawn seem to be unaffordable for the people in rural areas, especially for the mountainous ethnic minority in the North who are more independent to herbivorous and omnivorous fish such as grass carp, Indians carp, and common carp. Yen Chau is a district of Cypr...

  3. CARP Institutional Assessment in a Post-2008 Transition Scenario : Implications for Land Administration and Management

    OpenAIRE

    Marife Ballesteros; Felino Cortez

    2008-01-01

    The objective of this paper is to present the land administration and management (LAM) issues on CARP and determine the necessary institutional reforms on LAM in view of CARP expiration in 2008. The paper discussed the adverse effects brought about by weak land policy and poor land administration on attaining the objectives of CARP. The poor land records, the lack of information sharing among government land agencies, the tedious land titling and registration process, the unclear land policie...

  4. CARP Institutional Assessment in a Post-2008 Transition Scenario: Implications for Land Administration and Management (LAM)

    OpenAIRE

    Cortez, Felino; Ballesteros, Marife M.

    2008-01-01

    The objective of this paper is to present the land administration and management (LAM) issues on CARP and determine the necessary institutional reforms on LAM in view of CARP expiration in 2008. The paper discussed the adverse effects brought about by weak land policy and poor land administration on attaining the objectives of CARP. The poor land records, the lack of information sharing among government land agencies, the tedious land titling and registration process, the unclear land policie...

  5. RESEARCHES REGARDING THE TECHNOLOGICAL PERFORMANCES OF CARP REARING DURING WINTER PERIOD IN THE CONDITIONS OF A RECIRCULATING AQUACULTURE SYSTEM

    OpenAIRE

    G. STEFAN; Cristea, V.; A. MIHALACHE; Grecu, I.; I. VASILEAN; L. SFETCU; Gheorghe, D.; A. DOCAN

    2013-01-01

    The techniques of carp culture are highly diversified, ranging from the extensive production in pond or open water with no fertilization or supplemental feeding to highly intensive systems in concrete tanks or cages. Among the different carp species, common carp is the best species reared in intensive monoculture, the others (Chinese and Indian carps) being usually cultivated in polyculture (P. Kestemont, 1995). An experiment was conducted in inside recirculation system conditions to identify...

  6. The effect of tramadol hydrochloride on early life stages of fish.

    Science.gov (United States)

    Sehonova, Pavla; Plhalova, Lucie; Blahova, Jana; Berankova, Petra; Doubkova, Veronika; Prokes, Miroslav; Tichy, Frantisek; Vecerek, Vladimir; Svobodova, Zdenka

    2016-06-01

    The aim of this study was to perform the fish embryo acute toxicity test (FET) on zebrafish (Danio rerio) and the early-life stage toxicity test on common carp (Cyprinus carpio) with tramadol hydrochloride. The FET was performed using the method inspired by the OECD guideline 236. Newly fertilized zebrafish eggs were exposed to tramadol hydrochloride at concentrations of 10; 50; 100 and 200μg/l for a period of 144h. An embryo-larval toxicity test on C. carpio was performed according to OECD guideline 210 also with tramadol hydrochloride at concentrations 10; 50; 100 and 200μg/l for a period of 32 days. Hatching was significantly influenced in both acute and subchronic toxicity assays. Subchronic exposure also influenced early ontogeny, both morphometric and condition characteristics and caused changes in antioxidant enzyme activity. The LOEC value was found to be 10μg/l tramadol hydrochloride. PMID:27208654

  7. FEATURES OF PONDS ECOSYSTEM WHEN ECHINACEA PURPUREA (ECHINACEA PURPUREA L. MOENCH WERE USING IN CARP FEEDING

    Directory of Open Access Journals (Sweden)

    G. Dobrjanska

    2013-09-01

    Full Text Available Purpose. Definition fish productivity of the experimental ponds, fixed set of chemical parameters, that are specific to the environmental condition of water, which is the process of growing fish in a certain relation to it, and the level of accumulation of heavy metals in different organs and tissues of carp. Methodology. The ponds was three breed groups one-years carp average weight 39,7 g (hybrid of carp and wild carp, crossbreed frames carp, lyubin scaly carp with planting density 1000 ind./ha. Control group of carp was fed extruded feed containing 20 % protein, and research group ― the same feed, which was added in the manufacturing process, chopped dried Echinacea purpurea in the amount of 1 %. The duration of the experiment was 86 days. Definition of hydro-chemical parameters was performed by standard methods in analytical chemistry. Quantitative determination of the concentration of heavy metals in water and the organs and tissues of fish was performed by direct absorption solution in propane-butane air flames using absorption spectrophotometer C-115-M1. Findings. It was reviewed ecological status of water bodies. Found that when used in feeding carp Echinacea purpurea increased fish productivity, reduced cost of feed for growing. Chemical composition of experimental ponds water, while virtually unchanged. The comparative characteristics of heavy metals in organs and tissues carp in this part of the diet. Originality. At first time investigated the influence of Echinacea purpurea by adding it to feed on fish productivity, accumulation and distribution of heavy metals in organs and tissues of carp. Practical value. Fish productivity in the experimental ponds was higher by 20,4 % relative to control. Costs of feed per pound of gain decreased by 13,3 % when was used in fish feeding chopped dried Echinacea purpurea. Almost all metals accumulated in the organs and tissues of experimental groups of carp in a somewhat lesser extent.

  8. Invasive Bighead and Silver Carps Form Different Sized Shoals that Readily Intermix

    Science.gov (United States)

    Ghosal, Ratna; Xiong, Peter X.; Sorensen, Peter W.

    2016-01-01

    Two species of congeneric filter-feeding microphagous carps from Asia, the bighead and the silver carp, were recently introduced to North America and have become highly invasive. These species of carp have similar food habits but the silver carp has the unique habit of jumping when disturbed. Both species have complex but poorly understood social behaviors and while both are thought to aggregate (form groups) and shoal (form tight social groups), this possibility has not yet been examined in these species. The present study examined the grouping tendencies of these species in the laboratory and the effects of fish density and species identity on it. Using nearest neighbor distance (NND) as a metric, we showed that both juvenile bighead and juvenile silver carp grouped (aggregate) strongly (P0.05) on this behavior. Within aggregations, bighead carp tended to form a single large shoal while silver carp formed shoals of 2–3 individuals. Further, when tested as mixed-species groups, bighead and silver carp readily shoaled with each other but not with the common carp, which is from Eurasia and a member of another feeding guild. Due to their similar feeding strategies, we speculate that the bighead and silver carp tend to aggregate and shoal to facilitate both their foraging efforts and to avoid predation, while the differences in the size of the shoals they form may seemingly reflect their different anti-predation strategies. These complex shoaling behaviors likely influence Asian carp distribution in rivers, and thus how they might be sampled and managed. PMID:27276024

  9. Comparative Histomorphological Studies on Oesophagus of Catfish and Grass Carp

    OpenAIRE

    Enas A. Abd El Hafez; Doaa M. Mokhtar; Alaa Sayed Abou-Elhamd; Ahmed Hassan S. Hassan

    2013-01-01

    The present work was carried out on 40 specimens of oesophaguses of both sexes of catfish (carnivorous fish) and grass carp (herbivorous fish) in order to observe the morphological and histological differences between the two species. Oesophagus of catfish was divided into 2 parts: anterior and posterior ones. The anterior part of the oesophagus of catfish was characterized by the presence of numerous mucosal folds. It was lined by stratified epithelium with goblet cells. In addition to club ...

  10. Transforming Future Teaching through ‘Carpe Diem’ Learning Design

    OpenAIRE

    Gilly Salmon; Phemie Wright

    2014-01-01

    Academic staff in Higher Education (HE) need to transform their teaching practices to support more future-orientated, digital, student-centered learning. Promoting, enabling and implementing these changes urgently requires acceptable, meaningful and effective staff development for academics. We identify four key areas that are presenting as barriers to the implementation of successful staff development. We illuminate the Carpe Diem learning design workshop process and illustrate its impact on...

  11. Comparative Histomorphological Studies on Oesophagus of Catfish and Grass Carp

    Directory of Open Access Journals (Sweden)

    Enas A. Abd El Hafez

    2013-01-01

    Full Text Available The present work was carried out on 40 specimens of oesophaguses of both sexes of catfish (carnivorous fish and grass carp (herbivorous fish in order to observe the morphological and histological differences between the two species. Oesophagus of catfish was divided into 2 parts: anterior and posterior ones. The anterior part of the oesophagus of catfish was characterized by the presence of numerous mucosal folds. It was lined by stratified epithelium with goblet cells. In addition to club cells were observed in between the stratified epithelium. Scanning electron examination of the oesophageal epithelium of catfish demonstrated the presence of microvilli and fingerprint-like microridges in the superficial cell layer. The posterior part of the oesophagus of catfish was characterized by simple columnar mucus-secreting epithelium. The oesophagus of grass carp had shown the same structure along its entire length. It consisted of less folded mucosa than that observed in the oesophagus of catfish. The epithelium was characterized by the presence of taste buds. In conclusion, the present work revealed some differences in the structure of catfish oesophagus and grass carp oesophagus. These differences are related to type of food and feeding habits of each species.

  12. Hierarchical structure of the otolith of adult wild carp

    International Nuclear Information System (INIS)

    The otolith of adult wild carp contains a pair of asterisci, a pair of lappilli and a pair of sagittae. Current research works are mainly restricted to the field of the daily ring structure. The purpose of this work is to explore the structural characteristics of carp's otolith in terms of hierarchy from nanometer to millimeter scale by transmission election microscope (TEM) and scanning electron microscope (SEM). Based on the observation, carp's lapillus is composed of ordered aragonite crystals. Seven hierarchical levels of the microstructure were proposed and described with the scheme representing a complete organization in detail. SEM studies show not only the clear daily growth increment, but also the morphology within the single daily increment. The domain structure of crystal orientation in otolith was observed for the first time. Furthermore, TEM investigation displays that the lapillus is composed of aragonite crystals with nanometer scale. Four hierarchical levels of the microstructure of the sagitta are also proposed. The asteriscus which is composed of nanometer scale vaterite crystals is considered to have a uniform structure.

  13. Biotransformation and detoxication of molinate (Ordram) in fish

    International Nuclear Information System (INIS)

    Bioconcentration, deputation, and biotransformation of molinate were compared in common carp (cyprinus carpio), striped bass (Morone saxatilis), and white sturgeon (acipenser transmontanus) using a flow-through metabolism system. When compared to static conditions, flowing water improved oxygenation, decreased chemical volatilization and remetabolism, and run through a macroreticular resin, improved waste-product collection. Metabolite analysis employed gradient high-pressure liquid chromatography. Exposure to 100 μg L-1 [ring-14C]molinate for 24 h resulted in bioconcentration factors of 30.5 (carp), 25.3 (bass), and 19.7 (sturgeon); differences were not significant (all, P > 0.05). 14C depuration by common carp was significantly slower than that by either striped bass or white sturgeon (both, P < 0.01). All three species oxidized molinate to a number of products and hydrolyzed, or conjugated with glutathione (GSH), the sulfoxide or sulfone, ultimately producing the mercapturic acid; carp and sturgeon also formed a D-glucuronic acid conjugate. Common carp were significantly less capable of sulfoxidation and GSH conjugation than either striped bass (P < 0.05) or white sturgeon (P < 0.01). Therefore, the selective toxicity of molinate in carp may be due to less efficient depuration and metabolic deactivation

  14. More than Carpe Diem On the Theme of To his Coy Mistress

    Institute of Scientific and Technical Information of China (English)

    赵金凤

    2008-01-01

    Andrew Marvell's famous poem To his Coy Mistress is commonly considered a seduction poem with the theme of carpe diem.The poet's massive dependence on the sexual imagery,the images of time,eternity,and the decomposition,however,transcends the tradition of the usual carpe diem theme of seduction poem,unfolding the pessimism clad in the erotic overcoat.

  15. An analysis of inputs cost for carp farming sector in 2001 in Iran.

    Science.gov (United States)

    Salehi, Hassan

    2007-11-01

    Carp is widely sold and used in its fresh in Iran, however, recently a range of value additions may also be observed. It is essential to the sustainable development of a carp farm to know the production costs and their contribution. Warm-water fish farming is mainly based on common, silver, grass and bighead carp and the common carp and the three Chinese species are often reared in poly culture in Iran. Since, the 1970s carp farming has spread around the Caspian coast and farmed production reached a peak in 2006 with production of more than 73,400 tons. A study of production, costs and profitability of carp farming sector was carried out to help clarify carp production costs and their difference with location in 2001. A total of 101 farms from the three main carp farming provinces, Guilan, Mazandaran and Khuzestan were randomly selected, classified and studied. The results of the survey showed that the various producer provinces have different cost structures. Overall, feed and fertilizer with the highest level of variation accounted for 23% of total costs, followed by seed and labor and salary with 23 and 17%, respectively. On average, benefit-cost ratio and the rate of farm income were closely related to location. This result suggests that farmers practice more efficiently and have better conditions in Mazandaran, followed by Guilan province. PMID:19090234

  16. Formation and biological characterization of three new types of improved crucian carp

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The improved tetraploids (G1×AT) were obtained by distant crossing and gynogenesis and the high-body individuals accounted for 2% among G1×AT. After mating with each other, the high-body in- dividuals produced three kinds of bisexual fertile diploid fishes: high-body red crucian carp, high-body fork-like-tails goldfish and gray common carp. The high-body red crucian carp mating with each other formed three types of improved crucian carp (ICC) including improved red crucian carp (IRCC), im- proved color crucian carp (ICCC) and improved gray crucian carp (IGCC). The phenotypes, chromo- some numbers, gonadal structure and fertility of the three kinds of ICC and their offspring were observed. All the three kinds of ICC possessed some improved phenotypes such as higher body, smaller head and shorter tail. The ratios of the body height to body length of IRCC, ICCC and IGCC were 0.54, 0.51 and 0.54, respectively. All of them were obviously higher than that of red crucian carp 0.41 (P<0.01). Three kinds of ICC had the same chromosome number as red crucian carp with 100 chromosomes. All the ICC possessed normal gonads producing mature eggs or sperm, which was important for the production of an improved diploid population. Compared with red crucian carp, all the ICC had stronger fertility such as higher gametes production, higher fertilization rate and higher hatchery rate. Three types of improved diploid fish population were generated from the three kinds of ICC by self-crossing, respectively. The ICC can serve as ornamental fish and edible fish. They are also ideal parents to produce triploids by mating with tetraploids. The new ICC plays an important role in biological evolution and fish genetic breeding.

  17. Formation and biological characterization of three new types of improved curcian carp

    Institute of Scientific and Technical Information of China (English)

    WANG Jing; LIU Yun; QIN QinBo; CHEN Song; LIU ShaoJun; DUAN Wei; LIU JinHui; ZHANG Chun; LUO KaiKun; XIAO Jun

    2008-01-01

    The improved tetraploids (G1×AT) were obtained by distant crossing and gynogenesis and the high-body individuals accounted for 2% among G1×AT. After mating with each other, the high-body in-dividuals produced three kinds of bisexual fertile diploid fishes: high-body red crucian carp, high-body fork-like-tails goldfish and gray common carp. The high-body red crucian carp mating with each other formed three types of improved crucian carp (ICC) including improved red crucian carp (IRCC), im-proved color crucian carp (ICCC) and improved gray crucian carp (IGCC). The phenotypes, chromo-some numbers, gonadal structure and fertility of the three kinds of ICC and their offspring were observed. All the three kinds of ICC possessed some improved phenotypes such as higher body, smaller head and shorter tail. The ratios of the body height to body length of IRCC, ICCC and IGCC were 0.54, 0.51 and 0.54, respectively. All of them were obviously higher than that of red crucian carp 0.41 (P<0.01). Three kinds of ICC had the same chromosome number as red crucian carp with 100 chromosomes. All the ICC possessed normal gonads producing mature eggs or sperm, which was important for the production of an improved diploid population. Compared with red crucian carp, all the ICC had stronger fertility such as higher gametes production, higher fertilization rate and higher hatchery rate. Three types of improved diploid fish population were generated from the three kinds of ICC by self-crossing, respectively. The ICC can serve as ornamental fish and edible fish. They are also ideal parents to produce triploids by mating with tetraploids. The new ICC plays an important role in biological evolution and fish genetic breeding.

  18. Study on the Effects of Probiotics on the Growth of Cyprinus carpiod and Water Quality

    Institute of Scientific and Technical Information of China (English)

    Wanqing GUO; Lidan BAI; Hai WANG; Xiaowei LI; Wei SHI; Shuo SUN

    2015-01-01

    Objective] The aim was to discuss the effects of probiotics on the growth of Cyprinus carpiod and water quality. [Method] Taking C. carpiod as the research object, probiotics were supplemented in the fodder and water to study their effects on the growth of C. carpiod and water quality. [Result] Probiotics had promoting ef-fects on the growth of C. carpiod and its optimum dosage was 6%. pH, ammonia nitrogen content and nitrite content in water body in experimental groups were al lower than those in control group. [Conclusion] Compound probiotics had a broad application foreground in the aquatic breeding industry.

  19. Assessing impacts of introduced aquatic species: Grass carp in large systems

    Science.gov (United States)

    Bain, Mark B.

    1993-03-01

    Introduced species have created environmental benefits and unanticipated disasters so a priori assessments of species introductions are needed for environmental management. A checklist for assessing impacts of introduced species was developed from studies of introduced species and recommendations for planning introductions. Sterile, triploid grass carp ( Ctenopharyngodon idella) are just beginning to be used as a biocontrol agent for the management of aquatic vegetation in open waterways. Potential impacts of grass carp in open systems were identified by reviewing grass carp biology relative to the impact assessment checklist. The potential consequences of introduced grass carp were reviewed for one case study. The case study demonstrated that conclusions about potential impacts and monitoring needs can be made despite incomplete information and uncertainty. Indicators of environmental impact and vulnerability of host systems were grouped into six categories: population control, hybridization, diseases and parasites, habitat alterations, biological effects, and management issues. Triploid grass carp can significantly alter habitat and biological resources through the secondary effects of reductions in aquatic vegetation. Potential impacts and significant uncertainties involve fish dispersions from plant control areas, inability to control vegetation loss, loss of diverse plant communities and their dependent species, and conflicts with human use of the water resource. Adequate knowledge existed to assess most potential consequences of releasing large numbers of triploid grass carp in Guntersville Reservoir, Alabama. However, the assessment of potential impacts indicated that moderate, incremental stockings combined with monitoring of vegetation and biological resources are necessary to control the effects of grass carp and achieve desirable, intermediate plant densities.

  20. Sox genes in grass carp (Ctenopharyngodon idella with their implications for genome duplication and evolution

    Directory of Open Access Journals (Sweden)

    Tong Jingou

    2006-11-01

    Full Text Available Abstract The Sox gene family is found in a broad range of animal taxa and encodes important gene regulatory proteins involved in a variety of developmental processes. We have obtained clones representing the HMG boxes of twelve Sox genes from grass carp (Ctenopharyngodon idella, one of the four major domestic carps in China. The cloned Sox genes belong to group B1, B2 and C. Our analyses show that whereas the human genome contains a single copy of Sox4, Sox11 and Sox14, each of these genes has two co-orthologs in grass carp, and the duplication of Sox4 and Sox11 occurred before the divergence of grass carp and zebrafish, which support the "fish-specific whole-genome duplication" theory. An estimation for the origin of grass carp based on the molecular clock using Sox1, Sox3 and Sox11 genes as markers indicates that grass carp (subfamily Leuciscinae and zebrafish (subfamily Danioninae diverged approximately 60 million years ago. The potential uses of Sox genes as markers in revealing the evolutionary history of grass carp are discussed.