WorldWideScience

Sample records for cargo off-loading mechanism

  1. Gravity-Off-loading System for Large-Displacement Ground Testing of Spacecraft Mechanisms

    Science.gov (United States)

    Han, Olyvia; Kienholz, David; Janzen, Paul; Kidney, Scott

    2010-01-01

    Gravity-off-loading of deployable spacecraft mechanisms during ground testing is a long-standing problem. Deployable structures which are usually too weak to support their own weight under gravity require a means of gravity-off-loading as they unfurl. Conventional solutions to this problem have been helium-filled balloons or mechanical pulley/counterweight systems. These approaches, however, suffer from the deleterious effects of added inertia or friction forces. The changing form factor of the deployable structure itself and the need to track the trajectory of the center of gravity also pose a challenge to these conventional technologies. This paper presents a novel testing apparatus for high-fidelity zero-gravity simulation for special application to deployable space structures such as solar arrays, magnetometer booms, and robotic arms in class 100,000 clean room environments

  2. A systematic approach to pair secretory cargo receptors with their cargo suggests a mechanism for cargo selection by Erv14.

    Science.gov (United States)

    Herzig, Yonatan; Sharpe, Hayley J; Elbaz, Yael; Munro, Sean; Schuldiner, Maya

    2012-01-01

    The endoplasmic reticulum (ER) is the site of synthesis of secreted and membrane proteins. To exit the ER, proteins are packaged into COPII vesicles through direct interaction with the COPII coat or aided by specific cargo receptors. Despite the fundamental role of such cargo receptors in protein traffic, only a few have been identified; their cargo spectrum is unknown and the signals they recognize remain poorly understood. We present here an approach we term "PAIRS" (pairing analysis of cargo receptors), which combines systematic genetic manipulations of yeast with automated microscopy screening, to map the spectrum of cargo for a known receptor or to uncover a novel receptor for a particular cargo. Using PAIRS we followed the fate of ∼150 cargos on the background of mutations in nine putative cargo receptors and identified novel cargo for most of these receptors. Deletion of the Erv14 cargo receptor affected the widest range of cargo. Erv14 substrates have a wide array of functions and structures; however, they are all membrane-spanning proteins of the late secretory pathway or plasma membrane. Proteins residing in these organelles have longer transmembrane domains (TMDs). Detailed examination of one cargo supported the hypothesis that Erv14 dependency reflects the length rather than the sequence of the TMD. The PAIRS approach allowed us to uncover new cargo for known cargo receptors and to obtain an unbiased look at specificity in cargo selection. Obtaining the spectrum of cargo for a cargo receptor allows a novel perspective on its mode of action. The rules that appear to guide Erv14 substrate recognition suggest that sorting of membrane proteins at multiple points in the secretory pathway could depend on the physical properties of TMDs. Such a mechanism would allow diverse proteins to utilize a few receptors without the constraints of evolving location-specific sorting motifs.

  3. Cargo-shell and cargo-cargo couplings govern the mechanics of artificially loaded virus-derived cages

    Science.gov (United States)

    Llauró, Aida; Luque, Daniel; Edwards, Ethan; Trus, Benes L.; Avera, John; Reguera, David; Douglas, Trevor; Pablo, Pedro J. De; Castón, José R.

    2016-04-01

    Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two types of proteinaceous payloads. While bound cargo to the inner capsid surface mechanically reinforced the capsid in a structural manner, unbound cargo diffusing freely within the shell cavity pressurized the cages up to ~30 atm due to steric effects. Strong cargo-cargo coupling reduces the resilience of these nanocompartments in ~20% when bound to the shell. Understanding the stability of artificially loaded nanocages will help to design more robust and durable molecular nanocontainers.Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two

  4. The interaction of equine lysozyme:oleic acid complexes with lipid membranes suggests a cargo off-loading mechanism

    DEFF Research Database (Denmark)

    Nielsen, Søren Bang; Wilhelm, Kristina; Vad, Brian

    2010-01-01

    extent when free oleic acid (OA) was added, but not when free OA was removed from ELOA by prior incubation with bovine serum albumin, emphasizing the role of OA in this process. NMR data indicated an equilibrium between free and bound OA, which shifts towards free OA as ELOA is progressively diluted...

  5. Cargo-shell and cargo-cargo couplings govern the mechanics of artificially loaded virus-derived cages.

    Science.gov (United States)

    Llauró, Aida; Luque, Daniel; Edwards, Ethan; Trus, Benes L; Avera, John; Reguera, David; Douglas, Trevor; Pablo, Pedro J de; Castón, José R

    2016-04-28

    Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two types of proteinaceous payloads. While bound cargo to the inner capsid surface mechanically reinforced the capsid in a structural manner, unbound cargo diffusing freely within the shell cavity pressurized the cages up to ∼30 atm due to steric effects. Strong cargo-cargo coupling reduces the resilience of these nanocompartments in ∼20% when bound to the shell. Understanding the stability of artificially loaded nanocages will help to design more robust and durable molecular nanocontainers.

  6. Cargo adaptors: structures illuminate mechanisms regulating vesicle biogenesis.

    Science.gov (United States)

    Paczkowski, Jon E; Richardson, Brian C; Fromme, J Christopher

    2015-07-01

    Cargo adaptors sort transmembrane protein cargos into nascent vesicles by binding directly to their cytosolic domains. Recent studies have revealed previously unappreciated roles for cargo adaptors and regulatory mechanisms governing their function. The adaptor protein (AP)-1 and AP-2 clathrin adaptors switch between open and closed conformations that ensure they function at the right place at the right time. The exomer cargo adaptor has a direct role in remodeling the membrane for vesicle fission. Several different cargo adaptors functioning in distinct trafficking pathways at the Golgi are similarly regulated through bivalent binding to the ADP-ribosylation factor 1 (Arf1) GTPase, potentially enabling regulation by a threshold concentration of Arf1. Taken together, these studies highlight that cargo adaptors do more than just adapt cargos.

  7. Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin.

    Science.gov (United States)

    Snijder, Joost; Kononova, Olga; Barbu, Ioana M; Uetrecht, Charlotte; Rurup, W Frederik; Burnley, Rebecca J; Koay, Melissa S T; Cornelissen, Jeroen J L M; Roos, Wouter H; Barsegov, Valeri; Wuite, Gijs J L; Heck, Albert J R

    2016-08-08

    Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead, they have various protein-based organelles. These include bacterial microcompartments like the carboxysome and the virus-like nanocompartment encapsulin. Encapsulins have an adaptable mechanism for enzyme packaging, which makes it an attractive platform to carry a foreign protein cargo. Here we investigate the assembly pathways and mechanical properties of the cargo-free and cargo-loaded nanocompartments, using a combination of native mass spectrometry, atomic force microscopy and multiscale computational molecular modeling. We show that encapsulin dimers assemble into rigid single-enzyme bacterial containers. Moreover, we demonstrate that cargo encapsulation has a mechanical impact on the shell. The structural similarity of encapsulins to virus capsids is reflected in their mechanical properties. With these robust mechanical properties encapsulins provide a suitable platform for the development of nanotechnological applications.

  8. A mechanism for retromer endosomal coat complex assembly with cargo.

    Science.gov (United States)

    Harrison, Megan S; Hung, Chia-Sui; Liu, Ting-ting; Christiano, Romain; Walther, Tobias C; Burd, Christopher G

    2014-01-07

    Retromer is an evolutionarily conserved protein complex composed of the VPS26, VPS29, and VPS35 proteins that selects and packages cargo proteins into transport carriers that export cargo from the endosome. The mechanisms by which retromer is recruited to the endosome and captures cargo are unknown. We show that membrane recruitment of retromer is mediated by bivalent recognition of an effector of PI3K, SNX3, and the RAB7A GTPase, by the VPS35 retromer subunit. These bivalent interactions prime retromer to capture integral membrane cargo, which enhances membrane association of retromer and initiates cargo sorting. The role of RAB7A is severely impaired by a mutation, K157N, that causes Charcot-Marie-Tooth neuropathy 2B. The results elucidate minimal requirements for retromer assembly on the endosome membrane and reveal how PI3K and RAB signaling are coupled to initiate retromer-mediated cargo export.

  9. Curable off-loading footwear and methods

    DEFF Research Database (Denmark)

    2007-01-01

    Custom footbed devices, methods of constructing the devices and kits for constructing custom footbeds are disclosed that may be particularly advantageous in the treatment of plantar ulcers. The devices offer the advantage of off-loading pressure from the plantar ulcers in a device that is easy...

  10. Mechanized silica nanoparticles based on pillar[5]arenes for on-command cargo release.

    Science.gov (United States)

    Sun, Yu-Long; Yang, Ying-Wei; Chen, Dai-Xiong; Wang, Guan; Zhou, Yue; Wang, Chun-Yu; Stoddart, J Fraser

    2013-10-11

    Mechanized silica nanoparticles, equipped with pillar[5]arene-[2]pseudorotaxane nanovalves, operate in biological media to trap cargos within their nanopores, but release them when the pH is lowered or a competitive binding agent is added. Although cargo size plays an important role in cargo loading, cargo charge-type does not appear to have any significant influence on the amount of cargo loading or its release. These findings open up the possibility of using pillar[n]arene and its derivatives for the formation of robust and dynamic nanosystems that are capable of performing useful functions.

  11. 29 CFR Appendix III to Part 1918 - The Mechanics of Conventional Cargo Gear (Non-mandatory)

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false The Mechanics of Conventional Cargo Gear (Non-mandatory.... 1918, App. III Appendix III to Part 1918—The Mechanics of Conventional Cargo Gear (Non-mandatory) Note: This appendix is non-mandatory and provides an explanation of the mechanics in the correct spotting...

  12. Basic mechanisms for recognition and transport of synaptic cargos

    Directory of Open Access Journals (Sweden)

    Schlager Max A

    2009-08-01

    Full Text Available Abstract Synaptic cargo trafficking is essential for synapse formation, function and plasticity. In order to transport synaptic cargo, such as synaptic vesicle precursors, mitochondria, neurotransmitter receptors and signaling proteins to their site of action, neurons make use of molecular motor proteins. These motors operate on the microtubule and actin cytoskeleton and are highly regulated so that different cargos can be transported to distinct synaptic specializations at both pre- and post-synaptic sites. How synaptic cargos achieve specificity, directionality and timing of transport is a developing area of investigation. Recent studies demonstrate that the docking of motors to their cargos is a key control point. Moreover, precise spatial and temporal regulation of motor-cargo interactions is important for transport specificity and cargo recruitment. Local signaling pathways – Ca2+ influx, CaMKII signaling and Rab GTPase activity – regulate motor activity and cargo release at synaptic locations. We discuss here how different motors recognize their synaptic cargo and how motor-cargo interactions are regulated by neuronal activity.

  13. A hopping mechanism for cargo transport by molecular motors in crowded microtubules

    CERN Document Server

    Goldman, Carla

    2010-01-01

    Most models designed to study the bidirectional movement of cargos as they are driven by molecular motors rely on the idea that motors of different polarities can be coordinated by external agents if arranged into a motor-cargo complex to perform the necessary work [gross04]. Although these models have provided us with important insights into these phenomena, there are still many unanswered questions regarding the mechanisms through which the movement of the complex takes place on crowded microtubules. For example (i) how does cargo-binding affect motor motility? and in connection with that - (ii) how does the presence of other motors (and also other cargos) on the microtubule affect the motility of the motor-cargo complex? We discuss these questions from a different perspective. The movement of a cargo is conceived here as a hopping process resulting from the transference of cargo between neighboring motors. In the light of this, we examine the conditions under which cargo might display bidirectional movemen...

  14. Mechanisms for exporting large-sized cargoes from the endoplasmic reticulum.

    Science.gov (United States)

    Saito, Kota; Katada, Toshiaki

    2015-10-01

    Cargo proteins exported from the endoplasmic reticulum to the Golgi apparatus are typically transported in coat protein complex II (COPII)-coated vesicles of 60-90 nm diameter. Several cargo molecules including collagens and chylomicrons form structures that are too large to be accommodated by these vesicles, but their secretion still requires COPII proteins. Here, we first review recent progress on large cargo secretions derived especially from animal models and human diseases, which indicate the importance of COPII proteins. We then discuss the recent isolation of specialized factors that modulate the process of COPII-dependent cargo formation to facilitate the exit of large-sized cargoes from the endoplasmic reticulum. Based on these findings, we propose a model that describes the importance of the GTPase cycle for secretion of oversized cargoes. Next, we summarize reports that describe the structures of COPII proteins and how these results provide insight into the mechanism of assembly of the large cargo carriers. Finally, we discuss what issues remain to be solved in the future.

  15. Mechanized azobenzene-functionalized zirconium metal-organic framework for on-command cargo release

    Science.gov (United States)

    Meng, Xiangshi; Gui, Bo; Yuan, Daqiang; Zeller, Matthias; Wang, Cheng

    2016-01-01

    Stimuli-responsive metal-organic frameworks (MOFs) have gained increasing attention recently for their potential applications in many areas. We report the design and synthesis of a water-stable zirconium MOF (Zr-MOF) that bears photoresponsive azobenzene groups. This particular MOF can be used as a reservoir for storage of cargo in water, and the cargo-loaded MOF can be further capped to construct a mechanized MOF through the binding of β-cyclodextrin with the azobenzene stalks on the MOF surface. The resulting mechanized MOF has shown on-command cargo release triggered by ultraviolet irradiation or addition of competitive agents without premature release. This study represents a simple approach to the construction of stimuli-responsive mechanized MOFs, and considering mechanized UiO-68-azo made from biocompatible components, this smart system may provide a unique MOF platform for on-command drug delivery in the future. PMID:27493996

  16. Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin

    NARCIS (Netherlands)

    Snijder, Joost; Kononova, Olga; Barbu, Ioana M; Uetrecht, Charlotte; Rurup, W Frederik; Koay, Melissa S T; Burnley, Rebecca J; Cornelissen, Jeroen J L M; Roos, Wouter H; Barsegov, Valeri; Wuite, Gijs J L; Heck, Albert J R

    2016-01-01

    Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead, they have various protein-based organelles. These include bacterial microcompartments like the carboxysome and the virus-like nanocompartment encapsulin. Encapsulins have an adaptable mechanism for enz

  17. Assembly and mechanical properties of the cargo-free and cargo-loaded bacterial nanocompartment encapsulin

    NARCIS (Netherlands)

    Snijder, Joost; Kononova, Olga; Barbu, Ioana M; Uetrecht, Charlotte; Rurup, W Frederik; Burnley, Rebecca J; Koay, Melissa S T; Cornelissen, Jeroen J L M; Roos, Wouter H; Barsegov, Valeri; Wuite, Gijs J L; Heck, Albert J R

    2016-01-01

    Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead they have various protein-based organelles. These include bacterial microcompartments like the carboxysome and the virus-like nanocompartment encapsulin. Encapsulins have an adaptable mechanism for enzy

  18. Shuttle Orbiter Enterprise Off-Loaded at Redstone Arsenal Airfield

    Science.gov (United States)

    1978-01-01

    The Shuttle Orbiter Enterprise is off-loaded Redstone Arsenal Airfield for later Mated Vertical Ground Vibration tests (MVGVT) at Marshall Space Flight Center's Dynamic Test Stand. The tests marked the first time ever that the entire shuttle complement (including orbiter, external tank, and solid rocket boosters) were mated vertically.

  19. Tests Regarding the Transitory Regimes of Putting off Load of the Hydroagregate

    Directory of Open Access Journals (Sweden)

    Marcel Romulus Jurcu

    2015-09-01

    Full Text Available For high power and high revolution aggregates it is required that the operation should take place in safe conditions, for all the running regimes. The most difficult regimes for a hydro aggregate are the transitory regimes. Although, these regimes generally last a rather short period of time, for high power aggregates these regimes are accompanied by electric and mechanic solicitations which sometimes can surpass the admitted values. In the present paper, we present an evaluation method of the rotating parts’ dynamic stability of the hydro aggregates in the transitory regime of putting off load. We present the results obtained from the recordings of putting off load and those obtained after the dynamic equilibration of the hydro aggregate’s rotor.

  20. Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport.

    Science.gov (United States)

    Bai, Xiaobo; Karasmanis, Eva P; Spiliotis, Elias T

    2016-03-15

    Intracellular transport involves the regulation of microtubule motor interactions with cargo, but the underlying mechanisms are not well understood. Septins are membrane- and microtubule-binding proteins that assemble into filamentous, scaffold-like structures. Septins are implicated in microtubule-dependent transport, but their roles are unknown. Here we describe a novel interaction between KIF17, a kinesin 2 family motor, and septin 9 (SEPT9). We show that SEPT9 associates directly with the C-terminal tail of KIF17 and interacts preferentially with the extended cargo-binding conformation of KIF17. In developing rat hippocampal neurons, SEPT9 partially colocalizes and comigrates with KIF17. We show that SEPT9 interacts with the KIF17 tail domain that associates with mLin-10/Mint1, a cargo adaptor/scaffold protein, which underlies the mechanism of KIF17 binding to the NMDA receptor subunit 2B (NR2B). Significantly, SEPT9 interferes with binding of the PDZ1 domain of mLin-10/Mint1 to KIF17 and thereby down-regulates NR2B transport into the dendrites of hippocampal neurons. Measurements of KIF17 motility in live neurons show that SEPT9 does not affect the microtubule-dependent motility of KIF17. These results provide the first evidence of an interaction between septins and a nonmitotic kinesin and suggest that SEPT9 modulates the interactions of KIF17 with membrane cargo.

  1. Corrosion Mechanism of Corrosion-Resistant Steel Developed for Bottom Plate of Cargo Oil Tanks

    Institute of Scientific and Technical Information of China (English)

    Feilong SUN; Xiaogang LI; Fan ZHANG; Xuequn CHENG; Cheng ZHOU; Nianchun WU; Yuqun YIN

    2013-01-01

    A new type of corrosion-resistant steel consisting of ferrite and bainite phases was developed for cargo oil tanks of crude oil tankers.The corrosion rate of this new steel was 0.22 mm/a,which was equivalent to ca.1/5 of the criterion (≤ 1 mm/a) for corrosion-resistant steels.The composition and element distribution of the corrosion products were investigated by micro-Raman spectrometry and energy dispersive spectrometer.The results demonstrated that the corrosion product was composed of α-FeOOH,Fe3O4 and a continuous Cu enrichment layer.This kind of corrosion product was protective to the steel matrix and accounted for the enhancement of the corrosion resistance of the new developed steel.

  2. The Cargo

    DEFF Research Database (Denmark)

    Birch, Thomas; Charlton, Michael F.; Biggs, Lynn

    2014-01-01

    The Gresham ship was carrying a substantial cargo of different metals when she sank, representing a considerable financial loss. Reports record that some of that material recovered in 1846 included 2700 tin ingots, lead pigs and iron bars (British Archaeological Association, 1847, 361). The excav...

  3. 29 CFR 1917.114 - Cargo doors.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Cargo doors. 1917.114 Section 1917.114 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) MARINE TERMINALS Terminal Facilities § 1917.114 Cargo doors. (a) Mechanically operated. (1) Cargo...

  4. Mechanisms of Infectivity and Evasion Derived from Microvesicles Cargo Produced by Trypanosoma cruzi

    Science.gov (United States)

    Borges, Bruna C.; Uehara, Isadora A.; Dias, Laysa O. S.; Brígido, Paula C.; da Silva, Claudio V.; Silva, Marcelo J. B.

    2016-01-01

    Cell invasion by the intracellular protozoans requires interaction of proteins from both the host and the parasite. Many parasites establish chronic infections, showing they have the potential to escape the immune system; for example, Trypanosoma cruzi is an intracellular parasite that causes Chagas disease. Parasite internalization into host cell requires secreted and surface molecules, such as microvesicles. The release of microvesicles and other vesicles, such as exosomes, by different eukaryotic organisms was first observed in the late twentieth century. The characterization and function of these vesicles have recently been the focus of several investigations. In this review, we discuss the release of microvesicles by T. cruzi. The molecular content of these vesicles is composed of several molecules that take place during parasite-host cell interaction and contribute to the parasite-driven mechanism of evasion from the host immune system. These new findings appear to have a profound impact on the comprehension of T. cruzi biology and highlight novel potential strategies for developing more efficient therapeutic approaches. PMID:27921011

  5. Duration of off-loading and recurrence rate in Charcot osteo-arthropathy treated with less restrictive regimen with removable walker

    DEFF Research Database (Denmark)

    Christensen, Tomas M; Gade-Rasmussen, Birthe; Pedersen, Lis W

    2013-01-01

    Recent literature on acute diabetic Charcot osteoarthropathy (CA) reports unusually long periods of off-loading. Data suggest that this might increase the re-currence rate. Subsequently we evaluated the influence of duration of off-loading on the risk of required re-casting.......Recent literature on acute diabetic Charcot osteoarthropathy (CA) reports unusually long periods of off-loading. Data suggest that this might increase the re-currence rate. Subsequently we evaluated the influence of duration of off-loading on the risk of required re-casting....

  6. 46 CFR 154.1866 - Cargo hose connection: Transferring cargo.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo hose connection: Transferring cargo. 154.1866... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Operations § 154.1866 Cargo hose connection: Transferring cargo. No person may transfer cargo through a cargo hose...

  7. 46 CFR 154.534 - Cargo pumps and cargo compressors.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pumps and cargo compressors. 154.534 Section 154.534 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES... Equipment Cargo and Process Piping Systems § 154.534 Cargo pumps and cargo compressors. Cargo pumps...

  8. Communication: Cargo towing by artificial swimmers

    Science.gov (United States)

    Debnath, Debajyoti; Ghosh, Pulak K.; Li, Yunyun; Marchesoni, Fabio; Li, Baowen

    2016-11-01

    An active swimmer can tow a passive cargo by binding it to form a self-propelling dimer. The orientation of the cargo relative to the axis of the active dimer's head is determined by the hydrodynamic interactions associated with the propulsion mechanism of the latter. We show how the tower-cargo angular configuration greatly influences the dimer's diffusivity and, therefore, the efficiency of the active swimmer as a micro-towing motor.

  9. Perihelion Power Constraint and Wheel-Off-Loading Impact on BepiColombo's Relativity Experiment

    Science.gov (United States)

    Schuster, Anja Katharina; Jehn, Rüdiger

    2016-04-01

    In this paper the consequences of the Perihelion Power Constraint (PPC) are presented along with the impact of the Momentum Wheel-Off-loading (WOL) events on the estimation precision of the post-Newtonian parameters (PNP). This analysis will provide an estimate of the outcome of the relativity experiment in the framework of the joint mission between ESA and JAXA, named BepiColombo. The Mercury Planetary Orbiter (MPO), one of the two scientific elements of the composite spacecraft, aims to verify Einstein's theory of General Relativity plus alternative theories of gravitation to an unprecedented degree of accuracy [1]. The PPC describes the switch-off of the Ka-transponder when Mercury is ±35° around perihelion [2]. This causes a decrease in the quality of radiometric measurements. The implemented PPC distinguishes between different geometries such as inferior and superior constellations. Moreover, it differentiates between the case in which Mercury is close to superior solar conjunction (SSC) while being around perihelion and the one of only being in SSC. Our treatment has the essential effect that the standard deviation of range and doppler measurements is increased in the perihelion region by up to 1.83 ṡ 10-5 km/s (doppler noise). There is no clear trend to be seen in the resulting PNP uncertainties. For instance, the value of the estimation bias of the PNP γ of 8.232 ṡ 10-6 is almost by factor two larger than in the case when the PPC is not activated (4.243 ṡ 10-6). But applying the PPC to the back-up trajectory this trend is not confirmed, since the reverse is true for the γ uncertainty σ(γPPC) values of the covariance matrix that correspond to the spacecraft velocity components at each event. In our approximation there is no preferred direction of the parasitic ΔV. Instead, each direction receives a ΔV of √3- 3 cm/s per half a day and ΔV of √6- 3 cm/s per day [4]. There is a strong impact of the WOL to be seen on the achievable accuracy of

  10. Characterizing the composition of molecular motors on moving axonal cargo using "cargo mapping" analysis.

    Science.gov (United States)

    Neumann, Sylvia; Campbell, George E; Szpankowski, Lukasz; Goldstein, Lawrence S B; Encalada, Sandra E

    2014-10-30

    Understanding the mechanisms by which molecular motors coordinate their activities to transport vesicular cargoes within neurons requires the quantitative analysis of motor/cargo associations at the single vesicle level. The goal of this protocol is to use quantitative fluorescence microscopy to correlate ("map") the position and directionality of movement of live cargo to the composition and relative amounts of motors associated with the same cargo. "Cargo mapping" consists of live imaging of fluorescently labeled cargoes moving in axons cultured on microfluidic devices, followed by chemical fixation during recording of live movement, and subsequent immunofluorescence (IF) staining of the exact same axonal regions with antibodies against motors. Colocalization between cargoes and their associated motors is assessed by assigning sub-pixel position coordinates to motor and cargo channels, by fitting Gaussian functions to the diffraction-limited point spread functions representing individual fluorescent point sources. Fixed cargo and motor images are subsequently superimposed to plots of cargo movement, to "map" them to their tracked trajectories. The strength of this protocol is the combination of live and IF data to record both the transport of vesicular cargoes in live cells and to determine the motors associated to these exact same vesicles. This technique overcomes previous challenges that use biochemical methods to determine the average motor composition of purified heterogeneous bulk vesicle populations, as these methods do not reveal compositions on single moving cargoes. Furthermore, this protocol can be adapted for the analysis of other transport and/or trafficking pathways in other cell types to correlate the movement of individual intracellular structures with their protein composition. Limitations of this protocol are the relatively low throughput due to low transfection efficiencies of cultured primary neurons and a limited field of view available for

  11. Cargo recognition and cargo-mediated regulation of unconventional myosins.

    Science.gov (United States)

    Lu, Qing; Li, Jianchao; Zhang, Mingjie

    2014-10-21

    Organized motions are hallmarks of living organisms. Such motions range from collective cell movements during development and muscle contractions at the macroscopic scale all the way down to cellular cargo (e.g., various biomolecules and organelles) transportation and mechanoforce sensing at more microscopic scales. Energy required for these biological motions is almost invariably provided by cellular chemical fuels in the form of nucleotide triphosphate. Biological systems have designed a group of nanoscale engines, known as molecular motors, to convert cellular chemical fuels into mechanical energy. Molecular motors come in various forms including cytoskeleton motors (myosin, kinesin, and dynein), nucleic-acid-based motors, cellular membrane-based rotary motors, and so on. The main focus of this Account is one subfamily of actin filament-based motors called unconventional myosins (other than muscle myosin II, the remaining myosins are collectively referred to as unconventional myosins). In general, myosins can use ATP to fuel two types of mechanomotions: dynamic tethering actin filaments with various cellular compartments or structures and actin filament-based intracellular transport. In contrast to rich knowledge accumulated over many decades on ATP hydrolyzing motor heads and their interactions with actin filaments, how various myosins recognize their specific cargoes and whether and how cargoes can in return regulate functions of motors are less understood. Nonetheless, a series of biochemical and structural investigations in the past few years, including works from our own laboratory, begin to shed lights on these latter questions. Some myosins (e.g., myosin-VI) can function both as cellular transporters and as mechanical tethers. To function as a processive transporter, myosins need to form dimers or multimers. To be a mechanical tether, a monomeric myosin is sufficient. It has been shown for myosin-VI that its cellular cargo proteins can play critical roles

  12. 33 CFR 401.32 - Cargo booms-deck cargo.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Cargo booms-deck cargo. 401.32... OF TRANSPORTATION SEAWAY REGULATIONS AND RULES Regulations Seaway Navigation § 401.32 Cargo booms—deck cargo. (a) Every vessel shall have cargo booms secured in a manner that affords maximum...

  13. Scatter in cargo radiography.

    Science.gov (United States)

    Miller, Erin A; Caggiano, Joseph A; Runkle, Robert C; White, Timothy A; Bevill, Aaron M

    2011-03-01

    As a complement to passive detection systems, radiographic inspection of cargo is an increasingly important tool for homeland security because it has the potential to detect highly attenuating objects associated with special nuclear material or surrounding shielding, in addition to screening for items such as drugs or contraband. Radiographic detection of such threat objects relies on high image contrast between regions of different density and atomic number (Z). Threat detection is affected by scatter of the interrogating beam in the cargo, the radiographic system itself, and the surrounding environment, which degrades image contrast. Here, we estimate the extent to which scatter plays a role in radiographic imaging of cargo containers. Stochastic transport simulations were performed to determine the details of the radiography equipment and surrounding environment, which are important in reproducing measured data and to investigate scatter magnitudes for typical cargo. We find that scatter plays a stronger role in cargo radiography than in typical medical imaging scenarios, even for low-density cargo, with scatter-to-primary ratios ranging from 0.14 for very low density cargo, to between 0.20 and 0.40 for typical cargo, and higher yet for dense cargo.

  14. Cargo transport by several motors

    CERN Document Server

    Zhang, Yunxin

    2010-01-01

    In cells, organelles and vesicles are usually transported by cooperation of several motor proteins, including plus-end directed motor kinesin and minus-end directed motor dynein. Many biophysical models have been constructed to understand the mechanism of this motion. However, so far, the basic principle about it remains unclosed. In this paper, based on the recent experimental results and existing theoretical models, a spider-like model is provided. In this model, each motor is regarded as a bead-spring system. The bead can bind to or unbind from the track stochastically, and step forward or backward with fixed step size L and force dependent transition rates. The spring connects the bead to cargo tightly. At any time, the position of cargo is determined by force balance condition. The obvious characteristics of our model are that, the motors interact with each other and they do not share the external load equally. Our results indicate, the stall force of cargo, under which the mean velocity of cargo vanishe...

  15. Cargo work for maritime operations

    Energy Technology Data Exchange (ETDEWEB)

    House, D.

    2005-07-01

    Previously published as Kemp & Young's Cargo Work, this new edition has been revised and expanded to reflect unit load containerised systems that are now used in the majority of cargo shipping, while retaining the necessary detail on transporting key classes of cargoes safely, efficiently and profitably. The book covers general principles and the latest international regulations that affect all cargo work, including cargo types and key characteristics of a wide range of cargo classes. It also details Roll On-Roll Off cargo handling, containerisation, equipment and offshore supply. Chapter 4 on bulk cargoes contains a section headed: Coal, characteristics, categories and procedures, precautions.

  16. Multipurpose Cargo Transfer Bag

    Science.gov (United States)

    Broyan, James; Baccus, Shelley

    2014-01-01

    The Logistics Reduction (LR) project within the Advanced Exploration Systems (AES) program is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag, the suitcase-shaped common logistics carrying bag for Shuttle and the International Space Station. After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unzipped, unsnapped, and unfolded to be reused. Reuse ideas that have been investigated include partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing.

  17. The evaluation of off-loading using a new removable oRTHOsis in DIABetic foot (ORTHODIAB) randomized controlled trial: study design and rational

    OpenAIRE

    Mohammedi, Kamel; Potier, Louis; François, Maud; Dardari, Dured; Feron, Marilyne; Nobecourt-Dupuy, Estelle; Dolz, Manuel; Ducloux, Roxane; Chibani, Abdelkader; Eveno, Dominique-François; Crea Avila, Teresa; Sultan, Ariane; Baillet-Blanco, Laurence; Rigalleau, Vincent; Velho, Gilberto

    2016-01-01

    Background Off-loading is essential for diabetic foot management, but remains understudied. The evaluation of Off-loading using a new removable oRTHOsis in DIABetic foot (ORTHODIAB) trial aims to evaluate the efficacy of a new removable device “Orthèse Diabète” in the healing of diabetic foot. Methods/design ORTHODIAB is a French multi-centre randomized, open label trial, with a blinded end points evaluation by an adjudication committee according to the Prospective Randomized Open Blinded End...

  18. Air Cargo Security

    Science.gov (United States)

    2007-07-30

    passenger and all-cargo aircraft. The air cargo system consists of a large, complex distribution network linking manufacturers and shippers to freight...free transportation to San Jose, California. His only luggage was a guitar case that concealed hammers, mallets, a knife, and a spear gun. At the...authorities and airplane manufacturers study methods to mitigate the effects of in-flight explosions.76 The FAA has had a active research program in blast

  19. Groupage Cargo Transportation Model

    Directory of Open Access Journals (Sweden)

    Aleksejevs Ruslans

    2016-03-01

    Full Text Available In this work we consider a specific problem of optimal planning of maritime transportation of multiproduct cargo by ships of one (corporate strategy or several (partially corporate strategy companies: the core of the problem consists of the existence of the network of intermediate seaports (i.e. transitional seaports, where for every ship arrived the cargo handling is done, and which are situated between the starting and the finishing seaports. In this work, there are mathematical models built from scratch in the form of multicriteria optimization problem; then the goal attainment method of Gembicki is used for reducing the built models to a one-criterion problem of linear programming.

  20. Motility states in bidirectional cargo transport

    CERN Document Server

    Klein, Sarah; Santen, Ludger

    2015-01-01

    Intracellular cargos which are transported by molecular motors move stochastically along cytoskeleton filaments. In particular for bidirectionally transported cargos it is an open question whether the characteristics of their motion can result from pure stochastic fluctuations or whether some coordination of the motors is needed. The results of a mean-field model of cargo-motors dynamics, which was proposed by M\\"uller et al.[1] suggest the existence of high motility states which would result from a stochastic tug-of-war. Here we analyze a non-mean field extension of their model, that takes explicitly the position of each motor into account. We find that high motility states then disappear. We consider also a mutual motor-motor activation, as an explicit mechanism of motor coordination. We show that the results of the mean-field model are recovered only in case of a strong motor-motor activation in the limit of a high number of motors.

  1. 14 CFR 121.287 - Carriage of cargo in cargo compartments.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Carriage of cargo in cargo compartments....287 Carriage of cargo in cargo compartments. When cargo is carried in cargo compartments that are..., the cargo must be loaded so as to allow a crewmember to effectively reach all parts of the...

  2. 14 CFR 125.185 - Carriage of cargo in cargo compartments.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Carriage of cargo in cargo compartments... Requirements § 125.185 Carriage of cargo in cargo compartments. When cargo is carried in cargo compartments... during flight, the cargo must be loaded so as to allow a crewmember to effectively reach all parts of...

  3. Vacuolar transport in tobacco leaf epidermis cells involves a single route for soluble cargo and multiple routes for membrane cargo.

    Science.gov (United States)

    Bottanelli, Francesca; Foresti, Ombretta; Hanton, Sally; Denecke, Jürgen

    2011-08-01

    We tested if different classes of vacuolar cargo reach the vacuole via distinct mechanisms by interference at multiple steps along the transport route. We show that nucleotide-free mutants of low molecular weight GTPases, including Rab11, the Rab5 members Rha1 and Ara6, and the tonoplast-resident Rab7, caused induced secretion of both lytic and storage vacuolar cargo. In situ analysis in leaf epidermis cells indicates a sequential action of Rab11, Rab5, and Rab7 GTPases. Compared with Rab5 members, mutant Rab11 mediates an early transport defect interfering with the arrival of cargo at prevacuoles, while mutant Rab7 inhibits the final delivery to the vacuole and increases cargo levels in prevacuoles. In contrast with soluble cargo, membrane cargo may follow different routes. Tonoplast targeting of an α-TIP chimera was impaired by nucleotide-free Rha1, Ara6, and Rab7 similar to soluble cargo. By contrast, the tail-anchored tonoplast SNARE Vam3 shares only the Rab7-mediated vacuolar deposition step. The most marked difference was observed for the calcineurin binding protein CBL6, which was insensitive to all Rab mutants tested. Unlike soluble cargo, α-TIP and Vam3, CBL6 transport to the vacuole was COPII independent. The results indicate that soluble vacuolar proteins follow a single route to vacuoles, while membrane spanning proteins may use at least three different transport mechanisms.

  4. Acoustic Multipurpose Cargo Transfer Bag

    Science.gov (United States)

    Baccus, Shelley

    2015-01-01

    The Logistics Reduction (LR) project within the Advanced Exploration Systems (AES) program is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) are designed to be the same external volume as a regular cargo transfer bag, the common logistics carrier for the International Space Station. After use as a cargo bag, the MCTB can be unzipped and unfolded to be reused. This Acoustic MCTBs transform into acoustic blankets after the initial logistics carrying objective is complete.

  5. Stochastic Movement of Multiple Motor Transported Cargo

    Science.gov (United States)

    Ando, David; Gopinathan, Ajay; Xu, Jing

    2015-03-01

    Experimental observations of cargo position during transport by multiple motors are determined by several coupled stochastic processes. During collective transport, each motor can transition between multiple kinetic states, with the state of each motor influencing the states of the others via mechanical coupling through a common cargo. We measured the motion of a micron sized bead as it is transported by two kinesin motors along a single microtubule track, focusing on cargo displacements which are both axial and transverse to the microtubule. We model the effects of inter-motor interference and the state of each motor throughout time, and back out motor properties using a systematic comparison of experimental observations with simulated model traces over a wide parameter space. Our model captures a surface-associated mode of kinesin, which is only accessible via inter-motor interference in groups, in which kinesin diffuses along the microtubule surface and rapidly ``hops'' between protofilaments without dissociating from the microtubule. This enhances local exploration of the microtubule surface, possibly enabling cellular cargos to overcome macromolecular crowding and to navigate obstacles along micro- tubule tracks without sacrificing overall travel distance.

  6. 46 CFR 154.315 - Cargo pump and cargo compressor rooms.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pump and cargo compressor rooms. 154.315 Section... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Ship Arrangements § 154.315 Cargo pump and cargo compressor rooms. (a) Cargo pump rooms and...

  7. 46 CFR 154.1826 - Opening of cargo tanks and cargo sampling.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Opening of cargo tanks and cargo sampling. 154.1826... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Operations § 154.1826 Opening of cargo tanks and cargo sampling. (a) The master shall ensure that each cargo tank opening...

  8. Bidirectionality From Cargo Thermal Fluctuations in Motor-Mediated Transport

    CERN Document Server

    Miles, Christopher E

    2016-01-01

    Molecular motor proteins serve as an essential component of intracellular transport by generating forces to haul cargoes along cytoskeletal filaments. In some circumstances, two species of motors that are directed oppositely (e.g. kinesin, dynein) can be attached to the same cargo. The resulting net motion is known to be bidirectional, but the mechanism of switching remains unclear. In this work, we propose a mean-field mathematical model of the mechanical interactions of two populations of molecular motors with diffusion of the cargo (thermal fluctuations) as the fundamental noise source. By studying a simplified model, the delayed response of motors to rapid fluctuations in the cargo is quantified, allowing for the reduction of the full model to two "characteristic positions" of each of the motor populations. The system is then found to be "metastable", switching between two distinct directional transport states, or bidirectional motion. The time to switch between these states is then investigated using WKB...

  9. Cargo-cult training

    Science.gov (United States)

    Magueijo, João

    2009-12-01

    Richard Feynman, in one of his famous rants, evoked as a metaphor what he called "cargo-cult science". During the Second World War, the indigenous people of the South Pacific became accustomed to US Air Force planes landing on their islands, invariably bringing a profusion of desirable goods and tasty foods. When the war ended, they were distressed by the discontinuation of this popular service. So, they decided to take action. They cleared elongated patches of land to make them look like runways. They lit wood fires where they had seen electric floodlights guiding in the planes. They built a wooden shack and made a man sit inside with two halves of a coconut on each ear and bamboo bars sticking out like antennas: he was the "air controller". And they waited for the planes to return.

  10. 29 CFR 1918.84 - Bulling cargo.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Bulling cargo. 1918.84 Section 1918.84 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Handling Cargo § 1918.84 Bulling cargo. (a) Bulling cargo shall be done with the bull line led directly from the heel block. However, bulling may be done from...

  11. 78 FR 68784 - Cargo Securing Manuals

    Science.gov (United States)

    2013-11-15

    ... SECURITY Coast Guard 33 CFR Parts 97 and 160, and 46 CFR Part 97 RIN 1625-AA25 Cargo Securing Manuals... proposes requiring cargo securing manuals (CSMs) on vessels of 500 gross tons or more traveling on... Code of Federal Regulations CSAP Cargo safe access plan CSM Cargo Securing Manual CSS Code Code of...

  12. 46 CFR 153.930 - Cargo antidotes.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo antidotes. 153.930 Section 153.930 Shipping COAST... Cargo antidotes. No person may operate a tankship that carries a cargo listed in Table 1 unless the tankship has on board the antidotes described for the cargo in the Medical First Aid Guide for Use...

  13. 46 CFR 64.97 - Cargo hose.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Cargo hose. 64.97 Section 64.97 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MARINE PORTABLE TANKS AND CARGO HANDLING SYSTEMS Cargo Handling System § 64.97 Cargo hose. Each hose assembly, consisting of couplings and a...

  14. 46 CFR 151.03-9 - Cargo.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo. 151.03-9 Section 151.03-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Definitions § 151.03-9 Cargo. This term means any liquid, gas or solid having...

  15. 29 CFR 1918.53 - Cargo winches.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Cargo winches. 1918.53 Section 1918.53 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Vessel's Cargo Handling Gear § 1918.53 Cargo winches. (a...) There shall be no load other than the fall and cargo hook assembly on the winch when changing gears on...

  16. Clinical Efficacy and Safety of a New Method for Pressure Off-load for Patients with Diabetic Foot Syndrome: Ankle-foot Pneumoorthosis with TM Orlett

    Directory of Open Access Journals (Sweden)

    Galina Yurevna Strakhova

    2014-09-01

    Full Text Available AimThe purpose of this study was to assess the clinical efficacy, safety and consumer properties of ankle-foot pneumoorthosis with a HAS-337 TM Orlett compared with non-removable total contact cast (TCC immobilization.Materials and methodsOur study included 40 patients with diabetes mellitus type 1 (DM1 and type 2 (DM2 with neuropathic diabetic foot syndrome and chronic uninfected wounds of the plantar surface of the forefoot, with wound duration of at least 3 weeks, wound areas not less than 1 cm2 and wound depths not more than stage II based on Wagner’s classification. We excluded patients with infected wounds, osteomyelitis, Charcot osteoarthropathy or peripheral vascular disease. Our test group included 20 patients who received pressure off-load using ankle-foot pneumoorthosis with a HAS-337 TM Orlett. For a control group (n = 20, pressure off-load was achieved using TCC immobilization. Both groups were comparable with regard to age, gender, duration and degree of diabetes compensation and by original wound defect sizes (p >0.05. The study duration was 6 months. Plantar pressure was measured inside the orthosis or TCC and was compared with test shoe measurements. Our major criteria for pressure relief were reduced pressures in the wound area and the whole foot and the rate of wound healing.ResultsAt the end of the 6-month period, complete healing of all ulcers was achieved. The average healing time was 46.1±19.0 days for the test group and was 48.3±20.5 days for the control group (p >0.05. Two patients who wore pneumoorthosis with HAS-337 were discontinued upon patient request.With pneumoorthosis, the maximum peak pressure on the foot and wound defect areas was reduced by 26% and 57%, respectively. The pressure/time integral decreased on average by 41% (p >0.05. Furthermore, in the midfoot area with pneumoorthosis, the maximum pressure increased by 48% and the pressure/time integral increased by 47%.ConclusionsUsing pneumoorthosis with

  17. Proteomics Characterization of Exosome Cargo

    OpenAIRE

    Schey, Kevin L.; Luther, J. Matthew; Rose, Kristie L

    2015-01-01

    Characterization of exosomal cargo is of significant interest because this cargo can provide clues to exosome biogenesis, targeting, and cellular effects and may be a source of biomarkers for disease diagnosis, prognosis and response to treatment. With recent improvements in proteomics technologies, both qualitative and quantitative characterization of exosomal proteins is possible. Here we provide a brief review of exosome proteomics studies and provide detailed protocols for global qualitat...

  18. 46 CFR 154.1210 - Hold space, void space, cofferdam, and spaces containing cargo piping.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Hold space, void space, cofferdam, and spaces containing... Design, Construction and Equipment Cargo Area: Mechanical Ventilation System § 154.1210 Hold space, void space, cofferdam, and spaces containing cargo piping. (a) Each hold space, void space, cofferdam,...

  19. Cargo binding activates myosin VIIA motor function in cells.

    Science.gov (United States)

    Sakai, Tsuyoshi; Umeki, Nobuhisa; Ikebe, Reiko; Ikebe, Mitsuo

    2011-04-26

    Myosin VIIA, thought to be involved in human auditory function, is a gene responsible for human Usher syndrome type 1B, which causes hearing and visual loss. Recent studies have suggested that it can move processively if it forms a dimer. Nevertheless, it exists as a monomer in vitro, unlike the well-known two-headed processive myosin Va. Here we studied the molecular mechanism, which is currently unknown, of activating myosin VIIA as a cargo-transporting motor. Human myosin VIIA was present throughout cytosol, but it moved to the tip of filopodia upon the formation of dimer induced by dimer-inducing reagent. The forced dimer of myosin VIIA translocated its cargo molecule, MyRip, to the tip of filopodia, whereas myosin VIIA without the forced dimer-forming module does not translocate to the filopodial tips. These results suggest that dimer formation of myosin VIIA is important for its cargo-transporting activity. On the other hand, myosin VIIA without the forced dimerization module became translocated to the filopodial tips in the presence of cargo complex, i.e., MyRip/Rab27a, and transported its cargo complex to the tip. Coexpression of MyRip promoted the association of myosin VIIA to vesicles and the dimer formation. These results suggest that association of myosin VIIA monomers with membrane via the MyRip/Rab27a complex facilitates the cargo-transporting activity of myosin VIIA, which is achieved by cluster formation on the membrane, where it possibly forms a dimer. Present findings support that MyRip, a cargo molecule, functions as an activator of myosin VIIA transporter function.

  20. 46 CFR 153.908 - Cargo viscosity and melting point information; measuring cargo temperature during discharge...

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo viscosity and melting point information; measuring..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations Documents and Cargo Information § 153.908 Cargo viscosity and melting point information; measuring cargo temperature during discharge:...

  1. 46 CFR 154.1810 - Cargo manual.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo manual. 154.1810 Section 154.1810 Shipping COAST... SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Operations § 154.1810 Cargo manual. (a) No person... navigable waters of the United States, or a U.S. flag vessel, unless the vessel has on board a cargo...

  2. 46 CFR 153.907 - Cargo information.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo information. 153.907 Section 153.907 Shipping... Information § 153.907 Cargo information. (a) The master shall ensure that the following information for each... process for the vessel. (b) The master shall make sure that the following information for cargoes...

  3. 46 CFR 154.1858 - Cargo hose.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo hose. 154.1858 Section 154.1858 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Operations § 154.1858 Cargo hose. The person...

  4. 46 CFR 45.137 - Cargo ports.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Cargo ports. 45.137 Section 45.137 Shipping COAST GUARD....137 Cargo ports. (a) Unless otherwise authorized by the Commandant, the lower edge of any opening for cargo, personnel, machinery access, or similar opening in the side of a ship must be above a line...

  5. 46 CFR 28.885 - Cargo gear.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo gear. 28.885 Section 28.885 Shipping COAST GUARD... Aleutian Trade Act Vessels § 28.885 Cargo gear. (a) The safe working load (SWL) for the assembled gear shall be marked on the heel of each cargo boom, crane, or derrick. These letters and figures are to...

  6. 76 FR 51847 - Air Cargo Screening

    Science.gov (United States)

    2011-08-18

    ... Exporters and Importers, Cargo Airline Association, Council of Supply Chain Management Professionals... the air cargo supply chain. TSA Response: The 9/11 Act required the Secretary of Homeland Security to... conduct screening at stages earlier within the cargo supply chain and off-airport. Thus, the CCSP...

  7. 49 CFR 172.328 - Cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Cargo tanks. 172.328 Section 172.328... SECURITY PLANS Marking § 172.328 Cargo tanks. (a) Providing and affixing identification numbers. Unless a cargo tank is already marked with the identification numbers required by this subpart,...

  8. Cooperative behavior of molecular motors: Cargo transport and traffic phenomena

    Science.gov (United States)

    Lipowsky, Reinhard; Beeg, Janina; Dimova, Rumiana; Klumpp, Stefan; Müller, Melanie J. I.

    2010-01-01

    All eukaryotic cells including those of our own body contain complex transport systems based on molecular motors which walk along cytoskeletal filaments. These motors are rather small and make discrete mechanical steps with a step size of the order of 10 nm but are able to pull cargo particles over much larger distances, from micrometers up to meters. In vivo, the intracellular cargos include large membrane-bounded organelles, smaller vesicles, a subset of mRNAs, cytoskeletal filaments, and various protein building blocks, which are transported between different cell compartments. This cargo transport is usually performed by teams of motors. If all motors belong to the same molecular species, the cooperative action of the motors leads to uni-directional transport with a strongly increased run length and with a characteristic force dependence of the velocity distributions. If two antagonistic teams of motors pull on the same cargo particle, they perform a stochastic tug-of-war, which is characterized by a subtle force balance between the two motor teams and leads to several distinct patterns of bi-directional transport. So far, all experimental observations on bi-directional transport are consistent with such a tug-of-war. If many motors and/or cargo particles are transported along the filaments, one encounters various traffic phenomena. Depending on their mutual interactions and the compartment geometry, the motors form various spatio-temporal patterns such as traffic jams, and undergo nonequilibrium phase transitions between different patterns of transport.

  9. Anomalous motor mediated cargo transport in microtubule networks

    Science.gov (United States)

    Vandal, Steven; Macveigh-Fierro, Daniel; Shen, Zhiyuan; Lemoi, Kyle; Vidali, Luis; Ross, Jennifer; Tuzel, Erkan

    Cargo transport is an important biological mechanism by which cells locomote, self-organize, and actively transport organelles. This transport is mediated by the cytoskeletal network and molecular motors; however, it is not known how network self-organization and dynamics affect these transport processes. In order to develop a mechanistic understanding of cargo transport, we use a coarse-grained Brownian dynamics model that incorporates the dynamics of these networks, as well as experimentally determined motor properties. We will test these models with two experimental systems: (1) in vitro microtubule networks with kinesin-1 motors, and quantum dot cargos on recreated microtubule networks, and (2) an excellent model organism, the moss Physcomitrella patens, in which chloroplasts are transported via the microtubule network by means of kinesin-like proteins. Phenomenological network characterizations are made, both in vivo and in vitro, and cargo motility is characterized using Mean Squared Displacement (MSD) measurements. Our simulations shed light on the role of network density and motor properties on the observed transport behavior, and improve our understanding of cargo transport in cells.

  10. 46 CFR 153.970 - Cargo transfer piping.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo transfer piping. 153.970 Section 153.970 Shipping... § 153.970 Cargo transfer piping. The person in charge of cargo transfer shall ensure that: (a) Cargo is transferred to or from a cargo tank only through the tankship's cargo piping system; (b) Vapor not returned...

  11. 46 CFR 153.957 - Persons in charge of transferring liquid cargo in bulk or cleaning cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... safely transfer liquid cargo in bulk or to safely clean cargo tanks; (2) Each transfer of liquid cargo in... or cleaning cargo tanks. 153.957 Section 153.957 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... transferring liquid cargo in bulk or cleaning cargo tanks. (a) The owner and operator of the vessel, and his...

  12. Effect of fuel concentration on cargo transport by a team of Kinesin motors

    Science.gov (United States)

    Takshak, Anjneya; Mishra, Nirvantosh; Kulkarni, Aditi; Kunwar, Ambarish

    2017-02-01

    Eukaryotic cells employ specialized proteins called molecular motors for transporting organelles and vesicles from one location to another in a regulated and directed manner. These molecular motors often work collectively in a team while transporting cargos. Molecular motors use cytoplasmic ATP as fuel, which is hydrolyzed to generate mechanical force. While the effect of ATP concentration on cargo transport by single Kinesin motor function is well understood, it is still unexplored, both theoretically and experimentally, how ATP concentration would affect cargo transport by a team of Kinesin motors. For instance, how does fuel concentration affect the travel distances and travel velocities of cargo? How cooperativity of Kinesin motors engaged on a cargo is affected by ATP concentration? To answer these questions, here we develop mechano-chemical models of cargo transport by a team of Kinesin motors. To develop these models we use experimentally-constrained mechano-chemical model of a single Kinesin motor as well as earlier developed mean-field and stochastic models of load sharing for cargo transport. Thus, our new models for cargo transport by a team of Kinesin motors include fuel concentration explicitly, which was not considered in earlier models. We make several interesting predictions which can be tested experimentally. For instance, the travel distances of cargos are very large at limited ATP concentrations in spite of very small travel velocity. Velocities of cargos driven by multiple Kinesin have a Michaelis-Menten dependence on ATP concentration. Similarly, cooperativity among the engaged Kinesin motors on the cargo shows a Michaelis-Menten type dependence, which attains a maximum value near physiological ATP concentrations. Our new results can be potentially useful in controlling artificial nano-molecular shuttles precisely for targeted delivery in various nano-technological applications.

  13. Spatial partitioning of secretory cargo from Golgi resident proteins in live cells

    Directory of Open Access Journals (Sweden)

    White Jamie

    2001-10-01

    Full Text Available Abstract Background To maintain organelle integrity, resident proteins must segregate from itinerant cargo during secretory transport. However, Golgi resident enzymes must have intimate access to secretory cargo in order to carry out glycosylation reactions. The amount of cargo and associated membrane may be significant compared to the amount of Golgi membrane and resident protein, but upon Golgi exit, cargo and resident are efficiently sorted. How this occurs in live cells is not known. Results We observed partitioning of the fluorescent Golgi resident T2-CFP and fluorescent cargo proteins VSVG3-YFP or VSVG3-SP-YFP upon Golgi exit after a synchronous pulse of cargo was released from the ER. Golgi elements remained stable in overall size, shape and relative position as cargo emptied. Cargo segregated from resident rapidly by blebbing into micron-sized domains that contained little or no detectable resident protein and that appeared to be continuous with the parent Golgi element. Post-Golgi transport carriers (TCs exited repeatedly from these domains. Alternatively, entire cargo domains exited Golgi elements, forming large TCs that fused directly with the plasma membrane. However, domain formation did not appear to be an absolute prerequisite for TC exit, since TCs also exited directly from Golgi elements in the absence of large domains. Quantitative cargo-specific photobleaching experiments revealed transfer of cargo between Golgi regions, but no discrete intra-Golgi TCs were observed. Conclusions Our results establish domain formation via rapid lateral partitioning as a general cellular strategy for segregating different transmembrane proteins along the secretory pathway and provide a framework for consideration of molecular mechanisms of secretory transport.

  14. Proteomics characterization of exosome cargo.

    Science.gov (United States)

    Schey, Kevin L; Luther, J Matthew; Rose, Kristie L

    2015-10-01

    Characterization of exosomal cargo is of significant interest because this cargo can provide clues to exosome biogenesis, targeting, and cellular effects and may be a source of biomarkers for disease diagnosis, prognosis and response to treatment. With recent improvements in proteomics technologies, both qualitative and quantitative characterization of exosomal proteins is possible. Here we provide a brief review of exosome proteomics studies and provide detailed protocols for global qualitative, global quantitative, and targeted quantitative analysis of exosomal proteins. In addition, we provide an example application of a standard global quantitative analysis followed by validation via a targeted quantitative analysis of urine exosome samples from human patients. Advantages and limitations of each method are discussed as well as future directions for exosome proteomics analysis.

  15. Competitiveness of Slovenian Cargo Shipping

    Directory of Open Access Journals (Sweden)

    Milojka Počuča

    2012-10-01

    Full Text Available The paper presents Slovenian cargo shipping, its system oforganisation, ownership, age and value stntcture. It shows theimportance of shipping in the Slovenian overseas trade and itsimpact on the state's balance of payments. The analysis of thecompetitiveness of shipping was made from the viewpoint ofoperation costs and in comparison with the competitiveness ofEU shippers. The paper concludes with a proposal of possiblesystemic solutions which improve the competitiveness of Slovenianshipping.

  16. Structural Basis of Cargo Recognition by Unconventional Myosins in Cellular Trafficking.

    Science.gov (United States)

    Li, Jianchao; Lu, Qing; Zhang, Mingjie

    2016-08-01

    Unconventional myosins are a superfamily of actin-based molecular motors playing diverse roles including cellular trafficking, mechanical supports, force sensing and transmission, etc. The variable neck and tail domains of unconventional myosins function to bind to specific cargoes including proteins and lipid vesicles and thus are largely responsible for the diverse cellular functions of myosins in vivo. In addition, the tail regions, together with their cognate cargoes, can regulate activities of the motor heads. This review outlines the advances made in recent years on cargo recognition and cargo binding-induced regulation of the activity of several unconventional myosins including myosin-I, V, VI and X in cellular trafficking. We approach this topic by describing a series of high-resolution structures of the neck and tail domains of these unconventional myosins either alone or in complex with their specific cargoes, and by discussing potential implications of these structural studies on cellular trafficking of these myosin motors.

  17. NWA Cargo Is Granted Additional Cargo Frequencies to China Four all-cargo frequencies per week granted by U.S. Department of Transportation

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ NWA Cargo, the cargo division of Northwest Airlines, will expand its all-cargo freighter service between the United States and China after being awarded four additional frequencies per week by the U.S. Department of Transportation.

  18. 46 CFR 151.45-4 - Cargo-handling.

    Science.gov (United States)

    2010-10-01

    ... cargo hose connections. (4) Cargo transfer operations for any cargo requiring a PV or safety relief...) All connections for cargo transfer have been made to the vessel's fixed pipeline system. (7) In... control the operations as follows: (1) Supervise the operation of the cargo system valves. (2)...

  19. 46 CFR 154.1834 - Cargo transfer piping.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo transfer piping. 154.1834 Section 154.1834... piping. The person in charge of cargo transfer shall ensure that cargo is transferred to or from a cargo tank only through the cargo piping system....

  20. 46 CFR 153.910 - Cargo piping plan.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo piping plan. 153.910 Section 153.910 Shipping... Information § 153.910 Cargo piping plan. No person may operate a tankship unless the tankship has a cargo piping plan that: (a) Shows all cargo piping on the tankship; (b) Shows all cargo valving, pumps,...

  1. A propagating ATPase gradient drives transport of surface-confined cellular cargo.

    Science.gov (United States)

    Vecchiarelli, Anthony G; Neuman, Keir C; Mizuuchi, Kiyoshi

    2014-04-01

    The faithful segregation of duplicated genetic material into daughter cells is critical to all organisms. In many bacteria, the segregation of chromosomes involves transport of "centromere-like" loci over the main body of the chromosome, the nucleoid, mediated by a two-protein partition system: a nonspecific DNA-binding ATPase, ParA, and an ATPase stimulator, ParB, which binds to the centromere-like loci. These systems have previously been proposed to function through a filament-based mechanism, analogous to actin- or microtubule-based movement. Here, we reconstituted the F-plasmid partition system using a DNA-carpeted flow cell as an artificial nucleoid surface and magnetic beads coated with plasmid partition complexes as surface-confined cargo. This minimal system recapitulated directed cargo motion driven by a surface ATPase gradient that propagated with the cargo. The dynamics are consistent with a diffusion-ratchet model, whereby the cargo dynamically establishes, and interacts with, a concentration gradient of the ATPase. A chemophoresis force ensues as the cargo perpetually chases the ATPase gradient, allowing the cargo to essentially "surf" the nucleoid on a continuously traveling wave of the ATPase. Demonstration of this non-filament-based motility mechanism in a biological context establishes a distinct class of motor system used for the transport and positioning of large cellular cargo.

  2. Early endosomal escape of a cyclic cell-penetrating peptide allows effective cytosolic cargo delivery.

    Science.gov (United States)

    Qian, Ziqing; LaRochelle, Jonathan R; Jiang, Bisheng; Lian, Wenlong; Hard, Ryan L; Selner, Nicholas G; Luechapanichkul, Rinrada; Barrios, Amy M; Pei, Dehua

    2014-06-24

    Cyclic heptapeptide cyclo(FΦRRRRQ) (cFΦR4, where Φ is l-2-naphthylalanine) was recently found to be efficiently internalized by mammalian cells. In this study, its mechanism of internalization was investigated by perturbing various endocytic events through the introduction of pharmacologic agents and genetic mutations. The results show that cFΦR4 binds directly to membrane phospholipids, is internalized into human cancer cells through endocytosis, and escapes from early endosomes into the cytoplasm. Its cargo capacity was examined with a wide variety of molecules, including small-molecule dyes, linear and cyclic peptides of various charged states, and proteins. Depending on the nature of the cargos, they may be delivered by endocyclic (insertion of cargo into the cFΦR4 ring), exocyclic (attachment of cargo to the Gln side chain), or bicyclic approaches (fusion of cFΦR4 and cyclic cargo rings). The overall delivery efficiency (i.e., delivery of cargo into the cytoplasm and nucleus) of cFΦR4 was 4-12-fold higher than those of nonaarginine, HIV Tat-derived peptide, or penetratin. The higher delivery efficiency, coupled with superior serum stability, minimal toxicity, and synthetic accessibility, renders cFΦR4 a useful transporter for intracellular cargo delivery and a suitable system for investigating the mechanism of endosomal escape.

  3. Systems Analysis and Structural Design of an Unpressurized Cargo Delivery Vehicle

    Science.gov (United States)

    Wu, K. Chauncey; Cruz, Jonathan N.; Antol, Jeffrey; Sasamoto, Washito A.

    2007-01-01

    The International Space Station will require a continuous supply of replacement parts for ongoing maintenance and repair after the planned retirement of the Space Shuttle in 2010. These parts are existing line-replaceable items collectively called Orbital Replacement Units, and include heavy and oversized items such as Control Moment Gyroscopes and stowed radiator arrays originally intended for delivery aboard the Space Shuttle. Current resupply spacecraft have limited to no capability to deliver these external logistics. In support of NASA's Exploration Systems Architecture Study, a team at Langley Research Center designed an Unpressurized Cargo Delivery Vehicle to deliver bulk cargo to the Space Station. The Unpressurized Cargo Delivery Vehicle was required to deliver at least 13,200 lbs of cargo mounted on at least 18 Flight Releasable Attachment Mechanisms. The Crew Launch Vehicle design recommended in the Exploration Systems Architecture Study would be used to launch one annual resupply flight to the International Space Station. The baseline vehicle design developed here has a cargo capacity of 16,000 lbs mounted on up to 20 Flight Releasable Attachment Mechanisms. Major vehicle components are a 5.5m-diameter cargo module containing two detachable cargo pallets with the payload, a Service Module to provide propulsion and power, and an aerodynamic nose cone. To reduce cost and risk, the Service Module is identical to the one used for the Crew Exploration Vehicle design.

  4. Study on Alternative Cargo Launch Options from the Lunar Surface

    Energy Technology Data Exchange (ETDEWEB)

    Cheryl A. Blomberg; Zamir A. Zulkefli; Spencer W. Rich; Steven D. Howe

    2013-07-01

    In the future, there will be a need for constant cargo launches from Earth to Mars in order to build, and then sustain, a Martian base. Currently, chemical rockets are used for space launches. These are expensive and heavy due to the amount of necessary propellant. Nuclear thermal rockets (NTRs) are the next step in rocket design. Another alternative is to create a launcher on the lunar surface that uses magnetic levitation to launch cargo to Mars in order to minimize the amount of necessary propellant per mission. This paper investigates using nuclear power for six different cargo launching alternatives, as well as the orbital mechanics involved in launching cargo to a Martian base from the moon. Each alternative is compared to the other alternative launchers, as well as compared to using an NTR instead. This comparison is done on the basis of mass that must be shipped from Earth, the amount of necessary propellant, and the number of equivalent NTR launches. Of the options, a lunar coil launcher had a ship mass that is 12.7% less than the next best option and 17 NTR equivalent launches, making it the best of the presented six options.

  5. Stokes Efficiency of Molecular Motor-Cargo Systems

    Science.gov (United States)

    2008-01-01

    2655, 1994. 11 F. Reif , Fundamentals of Statistical and Thermal Physics, McGraw-Hill, New York, NY, USA, 1985. 12 H. Kleinert, Path Integrals in...Quantum Mechanics , Statistics , Polymer Physics, and Financial Markets, World Scientific, River Edge, NJ, USA, 3rd edition, 2004. 13 A. Einstein...Efficiency of Molecular Motor-Cargo Systems Hongyun Wang1 and Hong Zhou2 1Department of Applied Mathematics and Statistics , University of California

  6. OPTIMAL GOODS STOWAGE IN AIR CARGO TERMINALS

    Directory of Open Access Journals (Sweden)

    Gennadiy Yun

    2011-03-01

    Full Text Available Abstract. Lack of system research done by domestic and foreign scientists in the field of optimal cargostraffic distribution problem in Air Cargo Terminal, proves necessity of investigation and optimization of thisprocess. Mathematical models and algorithms of optimal problem solving for air cargos distribution inwarehouses on the basis of established functional dependence between freight flow stowage in warehousesand transport vehicle loading (unloading processes are presented in the article.Keywords:algorithms, dimensions of air cargo terminal, effectiveness of loading-unloading processes,freight flow, models.

  7. 46 CFR 153.977 - Supervision of cargo transfer.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Supervision of cargo transfer. 153.977 Section 153.977 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Procedures § 153.977 Supervision of cargo transfer. The person in charge of cargo transfer shall:...

  8. 46 CFR 154.552 - Cargo hose: Compatibility.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo hose: Compatibility. 154.552 Section 154.552 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY... Hose § 154.552 Cargo hose: Compatibility. Liquid and vapor cargo hoses must: (a) Not chemically...

  9. 46 CFR 153.935a - Storage of cargo samples.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Storage of cargo samples. 153.935a Section 153.935a Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... § 153.935a Storage of cargo samples. (a) The master shall make sure that any cargo samples are stored...

  10. 19 CFR 122.115 - Labeling of cargo.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Labeling of cargo. 122.115 Section 122.115 Customs... AIR COMMERCE REGULATIONS Transit Air Cargo Manifest (TACM) Procedures § 122.115 Labeling of cargo. A warning label, as required by § 18.4(e) of this chapter, shall be attached to all transit air cargo...

  11. 19 CFR 4.38 - Release of cargo.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Release of cargo. 4.38 Section 4.38 Customs Duties... VESSELS IN FOREIGN AND DOMESTIC TRADES Landing and Delivery of Cargo § 4.38 Release of cargo. (a) No... report which lists those shipments which have been authorized for release. This alternative cargo...

  12. 49 CFR 1548.21 - Screening of cargo.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Screening of cargo. 1548.21 Section 1548.21... cargo. An IAC may only screen cargo for transport on a passenger aircraft under §§ 1544.205 and 1546.205 if the IAC is a certified cargo screening facility as provided in part 1549....

  13. 46 CFR 151.45-6 - Maximum amount of cargo.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Maximum amount of cargo. 151.45-6 Section 151.45-6 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Operations § 151.45-6 Maximum amount of cargo. (a)...

  14. 19 CFR 4.33 - Diversion of cargo.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Diversion of cargo. 4.33 Section 4.33 Customs... VESSELS IN FOREIGN AND DOMESTIC TRADES Landing and Delivery of Cargo § 4.33 Diversion of cargo. (a) Unlading at other than original port of destination. A vessel may unlade cargo or baggage at an...

  15. 46 CFR 98.30-11 - Cargo pumps.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo pumps. 98.30-11 Section 98.30-11 Shipping COAST..., ARRANGEMENT, AND OTHER PROVISIONS FOR CERTAIN DANGEROUS CARGOES IN BULK Portable Tanks § 98.30-11 Cargo pumps. No person may operate a cargo pump to transfer a product to or from a portable tank unless the...

  16. 46 CFR 154.500 - Cargo and process piping standards.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo and process piping standards. 154.500 Section 154... Equipment Cargo and Process Piping Systems § 154.500 Cargo and process piping standards. The cargo liquid and vapor piping and process piping systems must meet the requirements in §§ 154.503 through...

  17. 46 CFR 153.251 - Independent cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Independent cargo tanks. 153.251 Section 153.251... CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Tanks § 153.251 Independent cargo tanks. All independent cargo tank must meet § 38.05-10 (a)(1), (b), (d),...

  18. 46 CFR 151.13-5 - Cargo segregation-tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo segregation-tanks. 151.13-5 Section 151.13-5... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-5 Cargo segregation—tanks. (a... symbols used in lines 1 and 2, in order, under the tank segregation column of Table 151.05....

  19. 46 CFR 105.20-3 - Cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo tanks. 105.20-3 Section 105.20-3 Shipping COAST... VESSELS DISPENSING PETROLEUM PRODUCTS Specific Requirements-Cargo Tanks § 105.20-3 Cargo tanks. (a) Construction and Materials. (1) The cargo tanks must be constructed of iron, steel, copper, nickel...

  20. 46 CFR 154.412 - Cargo tank corrosion allowance.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank corrosion allowance. 154.412 Section 154.412... Containment Systems § 154.412 Cargo tank corrosion allowance. A cargo tank must be designed with a corrosion...) carries a cargo that corrodes the tank material. Note: Corrosion allowance for independent tank type C...

  1. DNA hairpins promote temperature controlled cargo encapsulation in a truncated octahedral nanocage structure family

    Science.gov (United States)

    Franch, Oskar; Iacovelli, Federico; Falconi, Mattia; Juul, Sissel; Ottaviani, Alessio; Benvenuti, Claudia; Biocca, Silvia; Ho, Yi-Ping; Knudsen, Birgitta R.; Desideri, Alessandro

    2016-07-01

    In the present study we investigate the mechanism behind temperature controlled cargo uptake using a truncated octahedral DNA cage scaffold functionalized with one, two, three or four hairpin forming DNA strands inserted in one corner of the structure. This investigation was inspired by our previous demonstration of temperature controlled reversible encapsulation of the cargo enzyme, horseradish peroxidase, in the cage with four hairpin forming strands. However, in this previous study the mechanism of cargo uptake was not directly addressed (Juul, et al., Temperature-Controlled Encapsulation and Release of an Active Enzyme in the Cavity of a Self-Assembled DNA Nanocage, ACS Nano, 2013, 7, 9724-9734). In the present study we use a combination of molecular dynamics simulations and in vitro analyses to unravel the mechanism of cargo uptake in hairpin containing DNA cages. We find that two hairpin forming strands are necessary and sufficient to facilitate efficient cargo uptake, which argues against a full opening-closing of one corner of the structure being responsible for encapsulation. Molecular dynamics simulations were carried out to evaluate the atomistic motions responsible for encapsulation and showed that the two hairpin forming strands facilitated extension of at least one of the face surfaces of the cage scaffold, allowing entrance of the cargo protein into the cavity of the structure. Hence, the presented data demonstrate that cargo uptake does not involve a full opening of the structure. Rather, the uptake mechanism represents a feature of increased flexibility integrated in this nanocage structure upon the addition of at least two hairpin-forming strands.In the present study we investigate the mechanism behind temperature controlled cargo uptake using a truncated octahedral DNA cage scaffold functionalized with one, two, three or four hairpin forming DNA strands inserted in one corner of the structure. This investigation was inspired by our previous

  2. Design of heavy lift cargo aircraft

    Data.gov (United States)

    National Aeronautics and Space Administration — This is the bird of the skies of the future. The heavy lift cargo aircraft which is currently being developed by me has twice the payload capacity of an Antonov...

  3. OPTIMAL GOODS STOWAGE IN AIR CARGO TERMINALS

    OpenAIRE

    Yun, Gennadiy; Gyrych, Victoria

    2011-01-01

    Abstract. Lack of system research done by domestic and foreign scientists in the field of optimal cargostraffic distribution problem in Air Cargo Terminal, proves necessity of investigation and optimization of thisprocess. Mathematical models and algorithms of optimal problem solving for air cargos distribution inwarehouses on the basis of established functional dependence between freight flow stowage in warehousesand transport vehicle loading (unloading) processes are presented in the articl...

  4. Characterizing X-ray Attenuation of Containerized Cargo

    Energy Technology Data Exchange (ETDEWEB)

    Birrer, N. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Divin, C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Glenn, S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Martz, H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wang, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-08-02

    X-ray inspection systems can be used to detect radiological and nuclear threats in imported cargo. In order to better understand performance of these systems, the attenuation characteristics of imported cargo need to be determined. This project focused on developing image processing algorithms for segmenting cargo and using x-ray attenuation to quantify equivalent steel thickness to determine cargo density. These algorithms were applied to over 450 cargo radiographs. The results are summarized in this report.

  5. PDE6δ-mediated sorting of INPP5E into the cilium is determined by cargo-carrier affinity.

    Science.gov (United States)

    Fansa, Eyad Kalawy; Kösling, Stefanie Kristine; Zent, Eldar; Wittinghofer, Alfred; Ismail, Shehab

    2016-04-11

    The phosphodiesterase 6 delta subunit (PDE6δ) shuttles several farnesylated cargos between membranes. The cargo sorting mechanism between cilia and other compartments is not understood. Here we show using the inositol polyphosphate 5'-phosphatase E (INPP5E) and the GTP-binding protein (Rheb) that cargo sorting depends on the affinity towards PDE6δ and the specificity of cargo release. High-affinity cargo is exclusively released by the ciliary transport regulator Arl3, while low-affinity cargo is released by Arl3 and its non-ciliary homologue Arl2. Structures of PDE6δ/cargo complexes reveal the molecular basis of the sorting signal which depends on the residues at the -1 and -3 positions relative to farnesylated cysteine. Structure-guided mutation allows the generation of a low-affinity INPP5E mutant which loses exclusive ciliary localization. We postulate that the affinity to PDE6δ and the release by Arl2/3 in addition to a retention signal are the determinants for cargo sorting and enrichment at its destination.

  6. Cargo transport by cytoplasmic Dynein can center embryonic centrosomes.

    Directory of Open Access Journals (Sweden)

    Rafael A Longoria

    Full Text Available To complete meiosis II in animal cells, the male DNA material needs to meet the female DNA material contained in the female pronucleus at the egg center, but it is not known how the male pronucleus, deposited by the sperm at the periphery of the cell, finds the cell center in large eggs. Pronucleus centering is an active process that appears to involve microtubules and molecular motors. For small and medium-sized cells, the force required to move the centrosome can arise from either microtubule pushing on the cortex, or cortically-attached dynein pulling on microtubules. However, in large cells, such as the fertilized Xenopus laevis embryo, where microtubules are too long to support pushing forces or they do not reach all boundaries before centrosome centering begins, a different force generating mechanism must exist. Here, we present a centrosome positioning model in which the cytosolic drag experienced by cargoes hauled by cytoplasmic dynein on the sperm aster microtubules can move the centrosome towards the cell's center. We find that small, fast cargoes (diameter ∼100 nm, cargo velocity ∼2 µm/s are sufficient to move the centrosome in the geometry of the Xenopus laevis embryo within the experimentally observed length and time scales.

  7. Regulation of cargo-selective endocytosis by dynamin 2 GTPase-activating protein girdin.

    Science.gov (United States)

    Weng, Liang; Enomoto, Atsushi; Miyoshi, Hiroshi; Takahashi, Kiyofumi; Asai, Naoya; Morone, Nobuhiro; Jiang, Ping; An, Jian; Kato, Takuya; Kuroda, Keisuke; Watanabe, Takashi; Asai, Masato; Ishida-Takagishi, Maki; Murakumo, Yoshiki; Nakashima, Hideki; Kaibuchi, Kozo; Takahashi, Masahide

    2014-09-17

    In clathrin-mediated endocytosis (CME), specificity and selectivity for cargoes are thought to be tightly regulated by cargo-specific adaptors for distinct cellular functions. Here, we show that the actin-binding protein girdin is a regulator of cargo-selective CME. Girdin interacts with dynamin 2, a GTPase that excises endocytic vesicles from the plasma membrane, and functions as its GTPase-activating protein. Interestingly, girdin depletion leads to the defect in clathrin-coated pit formation in the center of cells. Also, we find that girdin differentially interacts with some cargoes, which competitively prevents girdin from interacting with dynamin 2 and confers the cargo selectivity for CME. Therefore, girdin regulates transferrin and E-cadherin endocytosis in the center of cells and their subsequent polarized intracellular localization, but has no effect on integrin and epidermal growth factor receptor endocytosis that occurs at the cell periphery. Our results reveal that girdin regulates selective CME via a mechanism involving dynamin 2, but not by operating as a cargo-specific adaptor.

  8. Failure of cargo aileron’s actuator

    Directory of Open Access Journals (Sweden)

    G. Zucca

    2014-10-01

    Full Text Available During a ferry flight, in a standard operation condition and at cruising level, a military cargo experienced a double hydraulic system failure due to a structural damage of the dual booster actuator. The booster actuator is the main component in mechanism of aileron’s deflection. The crew was able to arrange an emergency landing thanks to the spare oil onboard: load specialists refilled the hydraulic reservoirs. Due to safety concerns and in order to prevent the possibility of other similar incidents, a technical investigation took place. The study aimed to carry out the analysis of root causes of the actuator failure. The Booster actuator is composed mainly by the piston rod and its aluminum external case (AA7049. The assembly has two bronze caps on both ends. These are fixed in position by means of two retainers. At one end of the actuator case is placed a trunnion: a cylindrical protrusion used as a pivoting point on the aircraft. The fracture was located at one end of the case, on the trunnion side, in correspondence to the cap and over the retainer. One of the two fracture surfaces was found separated to the case and with the cap entangled inside. The fracture surfaces of the external case indicated fatigue crack growth followed by ductile separation. The failure analysis was performed by means of optical, metallographic, digital and electronic microscopy. The collected evidences showed a multiple initiation fracture mechanism. Moreover, 3D scanner reconstruction and numerical simulation demonstrated that dimensional non conformances and thermal loads caused an abnormal stress concentration. Stress concentration was located along the case assy outer surface where the fatigue crack originated. The progressive rupture mechanism grew under cyclical axial load due to the normal operations. Recommendations were issued in order to improve dimensional controls and assembly procedures during production and overhaul activities.

  9. Research on probability distribution of port cargo throughput

    Institute of Scientific and Technical Information of China (English)

    SUN Liang; TAN De-rong

    2008-01-01

    In order to more accurately examine developing trends in gross cargo throughput, we have modeled the probability distribution of cargo throughput. Gross cargo throughput is determined by the time spent by cargo ships in the port and the operating efficiency of handling equipment. Gross cargo throughput is the sum of all compound variables determining each aspect of cargo throughput for every cargo ship arriving at the port. Probability distribution was determined using the Wald equation. The results show that the variability of gross cargo throughput primarily depends on the different times required by different cargo ships arriving at the port. This model overcomes the shortcoming of previous models: inability to accurately determine the probability of a specific value of future gross cargo throughput. Our proposed model of cargo throughput depends on the relationship between time required by a cargo ship arriving at the port and the operational capacity of handling equipment at the port. At the same time, key factors affecting gross cargo throughput are analyzed. In order to test the efficiency of the model, the cargo volume of a port in Shandong Province was used as an example. In the case study the actual results matched our theoretical analysis.

  10. Uncertainty Quantification for Cargo Hold Fires

    CERN Document Server

    DeGennaro, Anthony M; Martinelli, Luigi; Rowley, Clarence W

    2015-01-01

    The purpose of this study is twofold -- first, to introduce the application of high-order discontinuous Galerkin methods to buoyancy-driven cargo hold fire simulations, second, to explore statistical variation in the fluid dynamics of a cargo hold fire given parameterized uncertainty in the fire source location and temperature. Cargo hold fires represent a class of problems that require highly-accurate computational methods to simulate faithfully. Hence, we use an in-house discontinuous Galerkin code to treat these flows. Cargo hold fires also exhibit a large amount of uncertainty with respect to the boundary conditions. Thus, the second aim of this paper is to quantify the resulting uncertainty in the flow, using tools from the uncertainty quantification community to ensure that our efforts require a minimal number of simulations. We expect that the results of this study will provide statistical insight into the effects of fire location and temperature on cargo fires, and also assist in the optimization of f...

  11. Optimization of the air cargo supply chain

    Directory of Open Access Journals (Sweden)

    María Pérez Bernal

    2012-10-01

    Full Text Available Purpose: This paper aims to evaluate and optimize the various operations within the air cargo chain. It pursues to improve the efficiency of the air cargo supply chain and to provide more information to the decision-makers to optimize their fields.Design/methodology/approach: The method used is a process simulation modelling software, WITNESS, which provides information to the decision-makers about the most relevant parameters subject to optimization. The input for the simulation is obtained from a qualitative analysis of the air cargo supply chain with the involved agents and from a study of the external trade by air mode, given that their behaviour depend on the location. The case study is focused on a particular location, the Case of Zaragoza Airport (Spain.Findings: This paper demonstrates that efficiency of the air cargo supply chain can increase by leveraging several parameters such as bottlenecks, resources or warehouses.Originality/value: It explores the use of a simulation modeling software originally intended for manufacturing processes and extended to support decision making processes in the area of air cargo.

  12. Dynamics modeling and control of large transport aircraft in heavy cargo extraction

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    In this paper,a novel version of six-degree-of-freedom nonlinear model for transport aircraft motion in cargo extraction is developed and validated by the theoretical mechanics and flight mechanics.In this model constraint force and moment reflecting the flight dynamic effects of inner moving cargo are formulated.A methodology for a control law design in this phase is presented,which linearizes the aircraft dynamics making use of piecewise linearization and utilizes robust control technique for interval sys...

  13. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy.

    Science.gov (United States)

    Sawa-Makarska, Justyna; Abert, Christine; Romanov, Julia; Zens, Bettina; Ibiricu, Iosune; Martens, Sascha

    2014-05-01

    Autophagy protects cells from harmful substances such as protein aggregates, damaged mitochondria and intracellular pathogens, and has been implicated in a variety of diseases. Selectivity of autophagic processes is mediated by cargo receptors that link cargo to Atg8 family proteins on the developing autophagosomal membrane. To avoid collateral degradation during constitutive autophagic pathways, the autophagic machinery must not only select cargo but also exclude non-cargo material. Here we show that cargo directly activates the cargo receptor Atg19 by exposing multiple Atg8 binding sites. Furthermore, Atg19 mediates tight apposition of the cargo and Atg8-coated membranes in a fully reconstituted system. These properties are essential for the function of Atg19 during selective autophagy in vivo. Our results suggest that cargo receptors contribute to tight membrane bending of the isolation membrane around the cargo.

  14. Characterizing Complexity of Containerized Cargo X-ray Images

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Guangxing [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Martz, Harry [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Glenn, Steven [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Divin, Charles [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Birrer, Nat [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-08-19

    X-ray imaging can be used to inspect cargos imported into the United States. In order to better understand the performance of X-ray inspection systems, the X-ray characteristics (density, complexity) of cargo need to be quantified. In this project, an image complexity measure called integrated power spectral density (IPSD) was studied using both DNDO engineered cargos and stream-of-commerce (SOC) cargos. A joint distribution of cargo density and complexity was obtained. A support vector machine was used to classify the SOC cargos into four categories to estimate the relative fractions.

  15. Fluctuation effects in bidirectional cargo transport

    CERN Document Server

    Klein, Sarah; Santen, Ludger

    2014-01-01

    We discuss a theoretical model for bidirectional cargo transport in biological cells, which is driven by teams of molecular motors and subject to thermal fluctuations. The model describes explicitly the directed motion of the molecular motors on the filament. The motor-cargo coupling is implemented via linear springs. By means of extensive Monte Carlo simulations we show that the model describes the experimentally observed regimes of anomalous diffusion, i.e. subdiffusive behavior at short times followed by superdiffusion at intermediate times. The model results indicate that subdiffuse regime is induced by thermal fluctuations while the superdiffusive motion is generated by correlations of the motors' activity. We also tested the efficiency of bidirectional cargo transport in crowded areas by measuring its ability to pass barriers with increased viscosity. Our results show a remarkable gain of efficiency for high viscosities.

  16. The iTRAPs: Guardians of Synaptic Vesicle Cargo Retrieval During Endocytosis.

    Science.gov (United States)

    Gordon, Sarah L; Cousin, Michael A

    2016-01-01

    The reformation of synaptic vesicles (SVs) during endocytosis is essential for the maintenance of neurotransmission in central nerve terminals. Newly formed SVs must be generated with the correct protein cargo in the correct stoichiometry to be functional for exocytosis. Classical clathrin adaptor protein complexes play a key role in sorting and clustering synaptic vesicle cargo in this regard. However it is becoming increasingly apparent that additional "fail-safe" mechanisms exist to ensure the accurate retrieval of essential cargo molecules. For example, the monomeric adaptor proteins AP180/CALM and stonin-2 are required for the efficient retrieval of synaptobrevin II (sybII) and synaptotagmin-1 respectively. Furthermore, recent studies have revealed that sybII and synaptotagmin-1 interact with other SV cargoes to ensure a high fidelity of retrieval. These cargoes are synaptophysin (for sybII) and SV2A (for synaptotagmin-1). In this review, we summarize current knowledge regarding the retrieval mechanisms for both sybII and synaptotagmin-1 during endocytosis. We also define and set criteria for a new functional group of SV molecules that facilitate the retrieval of their interaction partners. We have termed these molecules intrinsic trafficking partners (iTRAPs) and we discuss how the function of this group impacts on presynaptic performance in both health and disease.

  17. The iTRAPs: guardians of synaptic vesicle cargo retrieval during endocytosis

    Directory of Open Access Journals (Sweden)

    Sarah Louise Gordon

    2016-02-01

    Full Text Available The reformation of synaptic vesicles during endocytosis is essential for the maintenance of neurotransmission in central nerve terminals. Newly formed synaptic vesicles must be generated with the correct protein cargo in the correct stoichiometry to be functional for exocytosis. Classical clathrin adaptor protein complexes play a key role in sorting and clustering synaptic vesicle cargo in this regard. However it is becoming increasingly apparent that additional fail-safe mechanisms exist to ensure the accurate retrieval of essential cargo molecules. For example, the monomeric adaptor proteins AP180/CALM and stonin-2 are required for the efficient retrieval of synaptobrevin II and synaptotagmin-1 respectively. Furthermore, recent studies have revealed that synaptobrevin II and synaptotagmin-1 interact with other synaptic vesicle cargoes to ensure a high fidelity of retrieval. These cargoes are synaptophysin (for synaptobrevin II and SV2A (for synaptotagmin-1. In this review we summarise current knowledge regarding the retrieval mechanisms for both synaptobrevin II and synaptotagmin-1 during endocytosis. We also define and set criteria for a new functional group of synaptic vesicle molecules that facilitate the retrieval of their interaction partners. We have termed these molecules intrinsic trafficking partners (iTRAPs and we discuss how the function of this group impacts on presynaptic performance in both health and disease.

  18. Port Security: Container Cargo Control

    Directory of Open Access Journals (Sweden)

    Vladivoj Vlaković

    2006-05-01

    Full Text Available illicittrafficking of threat materials, especially explosives, chemicalsubstances and radioactive or nuclear material. The transportof the threat materials by using sea routes is an advantageto te"orists especially because of the possible use of ship containers.The container is the basis of world trade. It is assumed thatthe world total movement in containers is about 200 millionTEUs ("20-foot equivalent units" per year. The list of materialstransported by containers which should be subject to inspectionwith the aim of reducing the acts of te"orism includes explosives,narcotics, chemical weapons, hazardous chemicalsand radioactive materials.Of special interest is nuclear te"orism. The risk of nuclearte"orism carried out by sub-national groups should be considerednot only in the construction and/or use of nuclear device,but also in possible radioactive contamination of large urbanareas.The system of ship containers control is an essential componentof «smart border» concept. Modem personnel, parcel,vehicle and cargo inspection systems are non-invasive imagingtechniques based on the use of nuclear analytical techniques.The inspection systems use penetrating radiations: hard x-rays(300 keV or more or gamma-rays from radioactive sources(137Cs and 60Co with energies from 600 to 1300 keV that producea high resolution radiograph of the load. Unfortunately,this information is "non-specific" in that it gives no informationon the nature of objects that do not match_ the travel documentsand are not recognized by a visual analysis of the radiographicpicture. Moreover, there are regions of the containerwherex and gamma-ray systems are "blind" due to the high averageatomic number of the objects i"adiated that appear asblack spots in the radiographic image.The systems being developed are based on the use of fast, 14Me V, neutrons with detection of associated a-particle from nuclearreactionbywhichneutrons are produced (d+t>a+n.Jnsuch a way the possibility to

  19. National Security Science and Technology Initiative: Air Cargo Screening

    Energy Technology Data Exchange (ETDEWEB)

    Bingham, Philip R [ORNL; White, Tim [Pacific Northwest National Laboratory (PNNL); Cespedes, Ernesto [Idaho National Laboratory (INL); Bowerman, Biays [Brookhaven National Laboratory (BNL); Bush, John [Battelle

    2010-11-01

    explosive benchmarks combinations, and conducted modeling and simulation studies to begin populating the matrix of commodities, threats, and detection technologies. (4) Designed and fabricated basic (homogeneous) commodity test pallets and fabricated inert stimulants to support experiments and to validate modeling/simulation results. (5) Developed/expanded the team's capabilities to conduct full-scale imaging (neutron and x-ray) experiments of air cargo commodities and explosive benchmarks. (6) Conducted experiments to improve the collection of trace particles of explosives from a variety of surfaces representative of air cargo materials by means of mechanical (air/vibration/pressure), thermal, and electrostatic methods. Air cargo screening is a difficult challenge that will require significant investment in both research and development to find a suitable solution to ensure the safety of passengers without significantly hindering the flow of commodities. The initiative funded by Battelle has positioned this group to make major contributions in meeting the air cargo challenge by developing collaborations, developing laboratory test systems, improving knowledge of the challenges (both technical and business) for air cargo screening, and increasing the understanding of the capabilities for current inspection methods (x-ray radiography, x-ray backscatter, etc.) and potential future inspection methods (neutron radiography, fusion of detector modalities, advanced trace detection, etc.). Lastly, air cargo screening is still an issue that will benefit from collaboration between Department of Energy Laboratories and Battelle. On January 7, 2010, DHS Secretary Napolitano joined White House Press Secretary Robert Gibbs and Assistant to the President for Counterterrorism and Homeland Security John Brennan to announce several recommendations DHS has made to the President for improving the technology and procedures used to protect air travel from acts of terrorism. (This

  20. 46 CFR 153.440 - Cargo temperature sensors.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo temperature sensors. 153.440 Section 153.440 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Temperature Control Systems § 153.440 Cargo temperature sensors. (a) Except as prescribed in paragraph (c)...

  1. 33 CFR 157.23 - Cargo and ballast system information.

    Science.gov (United States)

    2010-07-01

    ... CARRYING OIL IN BULK Design, Equipment, and Installation § 157.23 Cargo and ballast system information. (a... automatic and manual operation of the cargo and ballast system in the vessel. (b) The format and information... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Cargo and ballast...

  2. 49 CFR 176.39 - Inspection of cargo.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Inspection of cargo. 176.39 Section 176.39... Requirements § 176.39 Inspection of cargo. (a) Manned vessels. The carrier, its agents, and any person... thereafter, weather permitting, in order to ensure that the cargo is in a safe condition and that no...

  3. 46 CFR 153.972 - Connecting a cargo hose.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Connecting a cargo hose. 153.972 Section 153.972 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations Cargo...

  4. 19 CFR 4.36 - Delayed discharge of cargo.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Delayed discharge of cargo. 4.36 Section 4.36... TREASURY VESSELS IN FOREIGN AND DOMESTIC TRADES Landing and Delivery of Cargo § 4.36 Delayed discharge of cargo. (a) When pursuant to section 457, Tariff Act of 1930, customs officers are placed on a...

  5. 46 CFR 98.25-80 - Cargo hose.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo hose. 98.25-80 Section 98.25-80 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS SPECIAL CONSTRUCTION, ARRANGEMENT, AND OTHER PROVISIONS FOR CERTAIN DANGEROUS CARGOES IN BULK Anhydrous Ammonia in Bulk §...

  6. 46 CFR 154.310 - Cargo piping systems.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo piping systems. 154.310 Section 154.310 Shipping... Arrangements § 154.310 Cargo piping systems. Cargo liquid or vapor piping must: (a) Be separated from other piping systems, except where an interconnection to inert gas or purge piping is required by §...

  7. 46 CFR 153.283 - Valving for cargo piping.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Valving for cargo piping. 153.283 Section 153.283... CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Piping Systems and Cargo Handling Equipment § 153.283 Valving for cargo piping. (a) Except as described in...

  8. 46 CFR 154.356 - Cargo emergency jettisoning piping.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo emergency jettisoning piping. 154.356 Section 154.356 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES... Equipment Ship Arrangements § 154.356 Cargo emergency jettisoning piping. Emergency jettisoning piping...

  9. 46 CFR 154.1844 - Cargo tanks: Filling limits.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tanks: Filling limits. 154.1844 Section 154.1844... STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Operations § 154.1844 Cargo tanks... cargo tank is not loaded: (1) More than 98 percent liquid full; or (2) In excess of the...

  10. 46 CFR 151.15-10 - Cargo gauging devices.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo gauging devices. 151.15-10 Section 151.15-10... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Tanks § 151.15-10 Cargo gauging devices. This section contains definitions and requirements for types of gauging devices specified in Table 151.05. (a)...

  11. 46 CFR 151.50-5 - Cargoes having toxic properties.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargoes having toxic properties. 151.50-5 Section 151.50... BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Special Requirements § 151.50-5 Cargoes having... transporting liquids having a Reid vapor pressure exceeding 14 pounds per square inch absolute or vented at...

  12. 46 CFR 151.25-2 - Cargo handling space.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo handling space. 151.25-2 Section 151.25-2 Shipping... BULK LIQUID HAZARDOUS MATERIAL CARGOES Environmental Control § 151.25-2 Cargo handling space. Pump rooms, compressor rooms, refrigeration rooms, heating rooms, instrument rooms or other closed...

  13. Linking molecular motors to membrane cargo

    NARCIS (Netherlands)

    A.S. Akhmanova (Anna); J.A. Hammer (John)

    2010-01-01

    textabstractThree types of motors, myosins, kinesins, and cytoplasmic dynein, cooperate to transport intracellular membrane organelles. Transport of each cargo is determined by recruitment of specific sets of motors and their regulation. Targeting of motors to membranes often depends on the formatio

  14. Cargo Integration Products for External Release

    Science.gov (United States)

    Sills, Joel W.

    2008-01-01

    This document consists of a listing of integration products by function: Reconfiguration Engineering, Payload Interface Requirements, and Cargo Integration Analysis. The products may be accessed from, the Flight Operation and Integration Office page, i.e. http://sspweb.jsc.nasa.gov/.

  15. DNA hairpins promote temperature controlled cargo encapsulation in a truncated octahedral nanocage structure family

    DEFF Research Database (Denmark)

    Franch, Oskar; Iacovelli, Federico; Falconi, Mattia

    2016-01-01

    and Release of an Active Enzyme in the Cavity of a Self-Assembled DNA Nanocage, ACS Nano, 2013, 7, 9724–9734). In the present study we use a combination of molecular dynamics simulations and in vitro analyses to unravel the mechanism of cargo uptake in hairpin containing DNA cages. We find that two hairpin...... forming strands are necessary and sufficient to facilitate efficient cargo uptake, which argues against a full opening–closing of one corner of the structure being responsible for encapsulation. Molecular dynamics simulations were carried out to evaluate the atomistic motions responsible for encapsulation...

  16. Discharging Part of LNG Liquid Cargo Handling Simulator

    Directory of Open Access Journals (Sweden)

    Zhen-Zhou Yang

    2013-09-01

    Full Text Available With the development of the national economy, it is important to develop LNG (Liquefied Natural Gas liquid cargo handling simulator. In this paper, the discharging part of Liquid Cargo Handling Simulator of LNG Carriers is developed by Visual Basic. The system includes 5 major forms and 3 mathematical models such as cargo pump model, the pressure and temperature model of the LNG vapour in the cargo tank and the liquid level model. The system can complete the unloading task. The system has become a preliminary model of LNG liquid cargo handlings simulator training.

  17. Aviation System Analysis Capability Air Carrier Investment Model-Cargo

    Science.gov (United States)

    Johnson, Jesse; Santmire, Tara

    1999-01-01

    The purpose of the Aviation System Analysis Capability (ASAC) Air Cargo Investment Model-Cargo (ACIMC), is to examine the economic effects of technology investment on the air cargo market, particularly the market for new cargo aircraft. To do so, we have built an econometrically based model designed to operate like the ACIM. Two main drivers account for virtually all of the demand: the growth rate of the Gross Domestic Product (GDP) and changes in the fare yield (which is a proxy of the price charged or fare). These differences arise from a combination of the nature of air cargo demand and the peculiarities of the air cargo market. The net effect of these two factors are that sales of new cargo aircraft are much less sensitive to either increases in GDP or changes in the costs of labor, capital, fuel, materials, and energy associated with the production of new cargo aircraft than the sales of new passenger aircraft. This in conjunction with the relatively small size of the cargo aircraft market means technology improvements to the cargo aircraft will do relatively very little to spur increased sales of new cargo aircraft.

  18. Cargo transportation by two species of motor protein.

    Science.gov (United States)

    Zhang, Yunxin

    2013-05-01

    The cargo motion in living cells transported by two species of motor protein with different intrinsic directionalities is discussed in this study. Similarly to single motor movement, the cargo steps forward and backward along a microtubule stochastically. Recent experiments found that cargo transportation by two motor species has a memory; it does not change its direction as frequently as expected, which means that its forward and backward step rates depend on its previous motion trajectory. By assuming that the cargo has only the least possible memory, i.e., its step direction depends only on the direction of its last step, two cases of cargo motion are analyzed in detail in this study: (I) cargo motion under constant external load, and (II) cargo motion in one fixed optical trap. Due to the existence of memory, in the first case, the cargo can keep moving in the same direction for a long distance. In the second case, the cargo will oscillate in the trap. The oscillation period decreases and the oscillation amplitude increases with increasing forward step rate of the motor, but both of them decrease with increasing trap stiffness. The most likely location of the cargo, where the probability of finding the oscillating cargo is maximum, may be the same as or may be different from the trap center, which depends on the step rates of the two motor species. Meanwhile, if the motors are robust, i.e., their forward to backward step rate ratios are high, there may be two such most likely locations, located one on each side of the trap center. The probability of finding the cargo in a given location, the probability of the cargo being in forward or backward motion, and various mean first passage times of the cargo to a given location or a given state are also analyzed.

  19. "Cargo-mooring" as an operating principle for molecular motors.

    Science.gov (United States)

    Lisowski, Bartosz; Kuśmierz, Łukasz; Żabicki, Michał; Bier, Martin

    2015-06-07

    Navigating through an ever-changing and unsteady environment, and utilizing chemical energy, molecular motors transport the cell׳s crucial components, such as organelles and vesicles filled with neurotransmitter. They generate force and pull cargo, as they literally walk along the polymeric tracks, e.g. microtubules. What we suggest in this paper is that the motor protein is not really pulling its load. The load is subject to diffusion and the motor may be doing little else than rectifying the fluctuations, i.e. ratcheting the load׳s diffusion. Below we present a detailed model to show how such ratcheting can quantitatively account for observed data. The consequence of such a mechanism is the dependence of the transport׳s speed and efficacy not only on the motor, but also on the cargo (especially its size) and on the environment (i.e. its viscosity and structure). Current experimental works rarely provide this type of information for in vivo studies. We suggest that even small differences between assays can impact the outcome. Our results agree with those obtained in wet laboratories and provide novel insight in a molecular motor׳s functioning.

  20. Cargo-mix optimization in Liner Shipping

    DEFF Research Database (Denmark)

    Christensen, Jonas Mark; Pacino, Dario; Fonseca, Joao Filipe Paiva

    , it is hard tomotivate companies to pay for cleaner transport since transportation is not visible to end customers,and therefore cannot justify a higher cost. Hence, optimization may be one of the few options forlimiting CO2 emission of international trade. A possible direction is to focus on vessels...... composition available in the different portsmight not be suitable for the full utilization of the vessel. The focus of our work is the analysis ofvessels’ cargo-mix, in particular finding what cargo composition is needed for a vessel to maximizeits utilization on a given service. Such a model can have various...... applications ranging from drivingrate prices, improving fleet composition and network design ((Christiansen et al., 2007; Reinhardtand Pisinger, 2012; Brouer et al., 2014))....

  1. A Review of Cargo Handling Equipment

    Science.gov (United States)

    1971-10-01

    surge, and sway). If, for example, only heave compensation is employed, i.e., one constraint, then the cargo may swing in a pendulous manner and the...pendulous mass as well as lack of heave compensation . One proposed method for overcoming the heave compensation problem is to tie the helicopter to the...deck. Further, any method which does not use the ship as a reference (i.e., not mounted on the ship) must also provide for some type of heave

  2. A modern-day solution to a 100-year-old problem: the use of a Bespoke Off-loading Brace in the rehabilitation of 'Deck-Slap' and other high-energy lower limb injuries.

    Science.gov (United States)

    Osborne, M A; Jarvis, H L; Bennett, N; Phillip, R D

    2014-01-01

    'Deck-Slap' is an injury pattern first described at the Battle of Jutland; it is still relevant today, with anti-vehicle mines a significant threat to Coalition troops. The effect of a device exploding beneath a vehicle produces a wave of high energy that is rapidly transmitted through the steel floor; this causes significant axial loading of lower limbs often resulting in severe fractures (notably of the calcaneum). Recent advancements in orthopaedic surgery have allowed for limbs that were destined for immediate amputation following significant trauma to be salvaged. However, despite intense rehabilitation, many of these salvaged limbs have subsequently gone on to delayed amputation, as functional outcomes are often poor. Technologically advanced prosthetic devices are available that afford good quality of life and allow for increased activity levels; these devices are, however, expensive to procure and maintain. This report describes a United Kingdom (UK) Armed Forces soldier who suffered a typical 'deck-slap' injury in Afghanistan with subsequent limb salvage. The use of the Bespoke Off-loading Brace (BOB) is discussed. The results presented here indicate that the biomechanical function of a patient with this type of injury improves when wearing the BOB. Further studies are needed to assess long-term clinical outcomes and the functional benefit of the device as a viable and cost-effective alternative to delayed limb amputation.

  3. Local synaptic signaling enhances the stochastic transport of motor-driven cargo in neurons

    KAUST Repository

    Newby, Jay

    2010-08-23

    The tug-of-war model of motor-driven cargo transport is formulated as an intermittent trapping process. An immobile trap, representing the cellular machinery that sequesters a motor-driven cargo for eventual use, is located somewhere within a microtubule track. A particle representing a motor-driven cargo that moves randomly with a forward bias is introduced at the beginning of the track. The particle switches randomly between a fast moving phase and a slow moving phase. When in the slow moving phase, the particle can be captured by the trap. To account for the possibility that the particle avoids the trap, an absorbing boundary is placed at the end of the track. Two local signaling mechanisms-intended to improve the chances of capturing the target-are considered by allowing the trap to affect the tug-of-war parameters within a small region around itself. The first is based on a localized adenosine triphosphate (ATP) concentration gradient surrounding a synapse, and the second is based on a concentration of tau-a microtubule-associated protein involved in Alzheimer\\'s disease-coating the microtubule near the synapse. It is shown that both mechanisms can lead to dramatic improvements in the capture probability, with a minimal increase in the mean capture time. The analysis also shows that tau can cause a cargo to undergo random oscillations, which could explain some experimental observations. © 2010 IOP Publishing Ltd.

  4. Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.

    Directory of Open Access Journals (Sweden)

    Shoko Itakura

    Full Text Available In anti-cancer therapy mediated by a nanoparticle-based drug delivery system (DDS, overall efficacy depends on the release efficiency of cargos from the nanoparticles in the cancer cells as well as the specificity of delivery to tumor tissue. However, conventional liposome-based DDS have no mechanism for specifically releasing the encapsulated cargos inside the cancer cells. To overcome this barrier, we developed nanoparticles containing a novel liposomal membrane destabilization peptide (LMDP that can destabilize membranes by cleavage with intramembranous proteases on/in cancer cells. Calcein encapsulated in liposomes modified with LMDP (LMDP-lipo was effectively released in the presence of a membrane fraction containing an LMDP-cleavable protease. The release was inhibited by a protease inhibitor, suggesting that LMDP-lipo could effectively release its cargo into cells in response to a cancer-specific protease. Moreover, when LMDP-lipo contained fusogenic lipids, the release of cargo was accelerated, suggesting that the fusion of LMDP-lipo with cellular membranes was the initial step in the intracellular delivery. Time-lapse microscopic observations showed that the release of cargo from LMDP-lipo occurred immediately after association of LMDP-lipo with target cells. Consequently, LMDP-lipo could be a useful nanoparticle capable of effective release of cargos specifically into targeted cancer cells.

  5. Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle.

    Science.gov (United States)

    Bertipaglia, Chiara; Schneider, Sarah; Jakobi, Arjen J; Tarafder, Abul K; Bykov, Yury S; Picco, Andrea; Kukulski, Wanda; Kosinski, Jan; Hagen, Wim Jh; Ravichandran, Arvind C; Wilmanns, Matthias; Kaksonen, Marko; Briggs, John Ag; Sachse, Carsten

    2016-07-01

    Selective autophagy is the mechanism by which large cargos are specifically sequestered for degradation. The structural details of cargo and receptor assembly giving rise to autophagic vesicles remain to be elucidated. We utilize the yeast cytoplasm-to-vacuole targeting (Cvt) pathway, a prototype of selective autophagy, together with a multi-scale analysis approach to study the molecular structure of Cvt vesicles. We report the oligomeric nature of the major Cvt cargo Ape1 with a combined 2.8 Å X-ray and negative stain EM structure, as well as the secondary cargo Ams1 with a 6.3 Å cryo-EM structure. We show that the major dodecameric cargo prApe1 exhibits a tendency to form higher-order chain structures that are broken upon interaction with the receptor Atg19 in vitro The stoichiometry of these cargo-receptor complexes is key to maintaining the size of the Cvt aggregate in vivo Using correlative light and electron microscopy, we further visualize key stages of Cvt vesicle biogenesis. Our findings suggest that Atg19 interaction limits Ape1 aggregate size while serving as a vehicle for vacuolar delivery of tetrameric Ams1.

  6. Installation of the Canadian Muon Cargo Inspection System at CRL

    Science.gov (United States)

    2014-04-01

    Installation of the Canadian Muon Cargo Inspection System at CRL Prepared by: Guy Jonkmans Atomic Energy of Canada Limited Chalk River ON...INSTALLATION OF THE CANADIAN MUON CARGO INSPECTION SYSTEM AT CRL 153-30100-REPT-001 Revision 0 2013/02/19 UNRESTRICTED 2013/02/19 ILLIMITÉ 153...30100-REPT-001 2013/02/19 Report, General Installation of the Canadian Muon Cargo Inspection System at CRL Research and Development 153-30100

  7. 46 CFR 150.160 - Carrying a cargo as an exception to the compatibility chart.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Carrying a cargo as an exception to the compatibility... BULK DANGEROUS CARGOES COMPATIBILITY OF CARGOES § 150.160 Carrying a cargo as an exception to the compatibility chart. The Operator of a vessel having on board a cargo carried as an exception under §...

  8. 46 CFR 308.517 - Open Cargo Policy, Form MA-300.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Open Cargo Policy, Form MA-300. 308.517 Section 308.517... Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.517 Open Cargo Policy, Form MA-300. The standard form of War Risk Open Cargo, Form MA-300, may be obtained from the American War...

  9. Cargo transportation by two species of motor protein

    CERN Document Server

    Zhang, Yunxin

    2012-01-01

    The cargo motion in living cells transported by two species of motor protein with different intrinsic directionality is discussed in this study. Similar to single motor movement, cargo steps forward and backward along microtubule stochastically. Recent experiments found that, cargo transportation by two motor species has a memory, it does not change its direction as frequently as expected, which means that its forward and backward step rates depends on its previous motion trajectory. By assuming cargo has only the least memory, i.e. its step direction depends only on the direction of its last step, two cases of cargo motion are detailed analyzed in this study: {\\bf (I)} cargo motion under constant external load; and {\\bf (II)} cargo motion in one fixed optical trap. Due to the existence of memory, for the first case, cargo can keep moving in the same direction for a long distance. For the second case, the cargo will oscillate in the trap. The oscillation period decreases and the oscillation amplitude increase...

  10. Column generation approaches to ship scheduling with flexible cargo sizes

    DEFF Research Database (Denmark)

    Brønmo, Geir; Nygreen, Bjørn; Lysgaard, Jens

    2010-01-01

    We present a Dantzig-Wolfe procedure for the ship scheduling problem with flexible cargo sizes. This problem is similar to the well-known pickup and delivery problem with time windows, but the cargo sizes are defined by intervals instead of by fixed values. The flexible cargo sizes have...... consequences for the times used in the ports because both the loading and unloading times depend on the cargo sizes. We found it computationally hard to find exact solutions to the subproblems, so our method does not guarantee to find the optimum over all solutions. To be able to say something about how good...

  11. Quantitative measurements and modeling of cargo-motor interactions during fast transport in the living axon

    Science.gov (United States)

    Seamster, Pamela E.; Loewenberg, Michael; Pascal, Jennifer; Chauviere, Arnaud; Gonzales, Aaron; Cristini, Vittorio; Bearer, Elaine L.

    2012-10-01

    The kinesins have long been known to drive microtubule-based transport of sub-cellular components, yet the mechanisms of their attachment to cargo remain a mystery. Several different cargo-receptors have been proposed based on their in vitro binding affinities to kinesin-1. Only two of these—phosphatidyl inositol, a negatively charged lipid, and the carboxyl terminus of the amyloid precursor protein (APP-C), a trans-membrane protein—have been reported to mediate motility in living systems. A major question is how these many different cargo, receptors and motors interact to produce the complex choreography of vesicular transport within living cells. Here we describe an experimental assay that identifies cargo-motor receptors by their ability to recruit active motors and drive transport of exogenous cargo towards the synapse in living axons. Cargo is engineered by derivatizing the surface of polystyrene fluorescent nanospheres (100 nm diameter) with charged residues or with synthetic peptides derived from candidate motor receptor proteins, all designed to display a terminal COOH group. After injection into the squid giant axon, particle movements are imaged by laser-scanning confocal time-lapse microscopy. In this report we compare the motility of negatively charged beads with APP-C beads in the presence of glycine-conjugated non-motile beads using new strategies to measure bead movements. The ensuing quantitative analysis of time-lapse digital sequences reveals detailed information about bead movements: instantaneous and maximum velocities, run lengths, pause frequencies and pause durations. These measurements provide parameters for a mathematical model that predicts the spatiotemporal evolution of distribution of the two different types of bead cargo in the axon. The results reveal that negatively charged beads differ from APP-C beads in velocity and dispersion, and predict that at long time points APP-C will achieve greater progress towards the presynaptic

  12. 46 CFR 151.20-10 - Cargo system instrumentation.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo system instrumentation. 151.20-10 Section 151.20-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES... instrumentation. (a) Each tank operated at other than ambient temperature shall be provided with at least...

  13. 46 CFR 154.548 - Cargo piping: Flow capacity.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo piping: Flow capacity. 154.548 Section 154.548... and Process Piping Systems § 154.548 Cargo piping: Flow capacity. Piping with an excess flow valve must have a vapor or liquid flow capacity that is greater than the rated closing flow under § 154.546....

  14. 49 CFR 175.78 - Stowage compatibility of cargo.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Stowage compatibility of cargo. 175.78 Section 175... Loading, Unloading and Handling § 175.78 Stowage compatibility of cargo. (a) For stowage on an aircraft..., Compatibility Group S, explosives are permitted to be transported aboard a passenger aircraft. Only...

  15. 49 CFR 180.405 - Qualification of cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... protection or other appropriate accident damage protection device. (v) A cargo tank may be constructed of.... (l) MC 300, MC 301, MC 302, MC 303, MC 305, MC 306 cargo tank—Rear accident damage protection. (1... accident damage protection device to protect the tank and piping in the event of a rear-end collision...

  16. 46 CFR 154.195 - Aluminum cargo tank: Steel enclosure.

    Science.gov (United States)

    2010-10-01

    ... published in “Rules for Building and Classing Steel Vessels”, 1981. (c) The steel cover for the aluminum... 46 Shipping 5 2010-10-01 2010-10-01 false Aluminum cargo tank: Steel enclosure. 154.195 Section... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction...

  17. Information tecnology and its impact on port cargo distribution businness

    OpenAIRE

    Trampus, Francesca

    2002-01-01

    The IT revolution has dynamised the port cargo business: up-to-date software is needed to optimize operations such as vessel loading, yard planning, equipment flow, gate handling, administration and invoicing and it is also essential for vessel tracking, electronic declaration of cargo and passengers and as a communication platform to all port users.

  18. 76 FR 41278 - Cargo Security Risk Reduction; Public Listening Sessions

    Science.gov (United States)

    2011-07-13

    ... SECURITY Coast Guard Cargo Security Risk Reduction; Public Listening Sessions AGENCY: Coast Guard, DHS..., transfer, and storage of Certain Dangerous Cargo (CDC) in bulk within the U.S. Marine Transportation System....Bergan@uscg.mil . SUPPLEMENTARY INFORMATION: Background In September 2009, the Coast Guard held a...

  19. 46 CFR 98.25-55 - Cargo piping.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo piping. 98.25-55 Section 98.25-55 Shipping COAST... Cargo piping. (a) Piping shall be of seamless steel meeting the requirements of § 56.60-1 of subchapter F (Marine Engineering) of this chapter. The piping shall be of not less than Schedule 40...

  20. Environmental control of microtubule-based bidirectional cargo-transport

    CERN Document Server

    Klein, Sarah; Santen, Ludger

    2014-01-01

    Inside cells, various cargos are transported by teams of molecular motors. Intriguingly, the motors involved generally have opposite pulling directions, and the resulting cargo dynamics is a biased stochastic motion. It is an open question how the cell can control this bias. Here we develop a model which takes explicitly into account the elastic coupling of the cargo with each motor. We show that bias can be simply controlled or even reversed in a counterintuitive manner via a change in the external force exerted on the cargo or a variation of the ATP binding rate to motors. Furthermore, the superdiffusive behavior found at short time scales indicates the emergence of motor cooperation induced by cargo-mediated coupling.

  1. 46 CFR 150.130 - Loading a cargo on vessels carrying cargoes with which it is incompatible.

    Science.gov (United States)

    2010-10-01

    ... it is incompatible. 150.130 Section 150.130 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... carrying cargoes with which it is incompatible. Except as described in § 150.160, the person in charge of a... any cargo in table I with which it is incompatible by two barriers such as formed by a: (1)...

  2. Transporting "exceptional cargo" on the CERN sites

    CERN Multimedia

    EN Department

    2012-01-01

    When the Transport Service is managing "exceptional cargo", the driver and the escort are often in charge of an operation involving equipment worth many hundred thousand francs. Equipment that may well be irreplaceable for a facility or an experiment.   The members of the Transport Service who carry out these tasks are very professional and are – needless to say – highly concentrated on the job. They count on your understanding and support in the traffic on site. Their convoys are – for good reasons – moving slowly. Kindly do not overtake, do not cut in in front of them and do not drive too closely. Respect the escort and do not position yourself between the truck and the escort vehicles. The EN department counts on your courtesy on the road.  

  3. On the possibility of the in-cell molecular motors working as cargo moorings only

    CERN Document Server

    Lisowski, Bartosz

    2012-01-01

    Although active transport is considered the most efficient way of depositing materials in the appropriate compartments within living cells, free diffusion remains the energetically cheapest and most widespread determinant of the in-cell dynamics. Here we investigate the hypothesis of cargoes - vesicles or organelles - being transported by the means of free diffusion, limited spatially by the molecular motors. Routinely navigating through ever-changing and unsteady environment, utilizing chemical energy (e.g. from hydrolyzing ATP), as the result they transport cell's crucial components, such as neurotransmitters and organelles. In our model the motor, which we identify with kinesin-1 walking along the microtubule, tethers the cargo in the same manner as a mooring rope attached to a bollard holds a ship from drifting toward the open sea. We study the model behavior in the context of optical trap single molecule experiments, mimicking their procedures in our simulations and investigate how the mechanical propert...

  4. Neutron and Photon Transport in Sea-Going Cargo Containers

    Energy Technology Data Exchange (ETDEWEB)

    Pruet, J; Descalle, M; Hall, J; Pohl, B; Prussin, S G

    2005-02-09

    Factors affecting sensing of small quantities of fissionable material in large sea-going cargo containers by neutron interrogation and detection of {beta}-delayed photons are explored. The propagation of variable-energy neutrons in cargos, subsequent fission of hidden nuclear material and production of the {beta}-delayed photons, and the propagation of these photons to an external detector are considered explicitly. Detailed results of Monte Carlo simulations of these stages in representative cargos are presented. Analytical models are developed both as a basis for a quantitative understanding of the interrogation process and as a tool to allow ready extrapolation of the results to cases not specifically considered here.

  5. 19 CFR 122.117 - Requirements for transit air cargo transport.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Requirements for transit air cargo transport. 122... Requirements for transit air cargo transport. (a) Transportation—(1) Port to port. Transit air cargo may be... cargo, a receipt shall be given. The receipt shall be made by the airline responsible for transport...

  6. 49 CFR 178.320 - General requirements applicable to all DOT specification cargo tank motor vehicles.

    Science.gov (United States)

    2010-10-01

    ...) Each cargo tank or cargo tank motor vehicle design type, including its required accident damage... specification cargo tank motor vehicles. 178.320 Section 178.320 Transportation Other Regulations Relating to... Transportation § 178.320 General requirements applicable to all DOT specification cargo tank motor vehicles....

  7. Determinan Pilihan Pelanggan Terhadap Layanan Cargo Pada PT. Garuda Indonesia Cabang Makassar

    OpenAIRE

    RESPATI., TRIADHI

    2012-01-01

    2012 Penelitian ini bertujuan untuk menganalisis perbedaan pilihan pelanggan terhadap layanan cargo dan memperoleh faktor perbedaan dalam keputusan pilihan pelanggan terhadap layanan cargo. Sedangkan metode analisis yang digunakan adalah analisis deskriptif, analisis kuantitatif dan uji hipotesis. Berdasarkan hasil analisis perbedaan pilihan pelanggan layanan cargo dari masing-masing perusahaan cargo (GIA, Lion Air, Sriwijaya Air dan PT. Wings Air) ternyata ada perbedaan yang signifikan...

  8. 46 CFR 153.436 - Heat transfer fluids: compatibility with cargo.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Heat transfer fluids: compatibility with cargo. 153.436... Equipment Cargo Temperature Control Systems § 153.436 Heat transfer fluids: compatibility with cargo. A heat transfer fluid separated from the cargo by only one wall (for example, the heat transfer fluid in a...

  9. Stochastic modeling of cargo transport by teams of molecular motors

    CERN Document Server

    Klein, Sarah; Santen, Ludger

    2014-01-01

    Many different types of cellular cargos are transported bidirectionally along microtubules by teams of molecular motors. The motion of this cargo-motors system has been experimentally characterized in vivo as processive with rather persistent directionality. Different theoretical approaches have been suggested in order to explore the origin of this kind of motion. An effective theoretical approach, introduced by M\\"uller et al., describes the cargo dynamics as a tug-of-war between different kinds of motors. An alternative approach has been suggested recently by Kunwar et al., who considered the coupling between motor and cargo in more detail. Based on this framework we introduce a model considering single motor positions which we propagate in continuous time. Furthermore, we analyze the possible influence of the discrete time update schemes used in previous publications on the system's dynamic.

  10. Cargo Loading Facilities, Published in unknown, Arizona Department of Transportation.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Cargo Loading Facilities dataset as of unknown. Data by this publisher are often provided in State Plane coordinate system; in a Transverse Mercator projection;...

  11. Cargo/Weapons Elevator Land Based Engineering Site

    Data.gov (United States)

    Federal Laboratory Consortium — The Cargo and Weapons Facility consists of a suite of full scale and component test facilities contiguously located in building 77H. The site was constructed in 1987...

  12. Aggregating Secondary Source Data for Air Cargo Prescreening Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose to improve the effectiveness of air cargo prescreening by enabling background knowledge about companies and products to be used for threat assessment. The...

  13. Design and Inspection of Liquid Cargo Piping of Chemical Tanker

    Institute of Scientific and Technical Information of China (English)

    Ying Sheng; Jiang Chengsong

    2012-01-01

    There is a great variety of liquid chemicals, and many shipowners are not clear about the cargos their ships will carry at the early stage of the design and construction of chemical tankers. In addition, because of the inclination to choose low cost construction material due to cost concern, the sources of cargos that ships can carry are greatly limited and the operation efficiency will be influenced. What should we do to avoid such situations?

  14. Reducing Aerodynamic Drag on Empty Open Cargo Vehicles

    Science.gov (United States)

    Ross, James C.; Storms, Bruce L.; Dzoan, Dan

    2009-01-01

    Some simple structural modifications have been demonstrated to be effective in reducing aerodynamic drag on vehicles that have empty open cargo bays. The basic idea is to break up the airflow in a large open cargo bay by inserting panels to divide the bay into a series of smaller bays. In the case of a coal car, this involves inserting a small number (typically between two and four) of vertical full-depth or partial-depth panels.

  15. SIMULATIONS FOR ACTIVE INTERROGATION OF HEU IN CARGO CONTAINERS

    Energy Technology Data Exchange (ETDEWEB)

    LEE, SANG Y. [Los Alamos National Laboratory; BEDDINGFIELD, DAVID H. [Los Alamos National Laboratory; PARK, JAEYOUNG [Los Alamos National Laboratory

    2007-01-22

    We describe the results of a Monte Carlo simulation 10 investigate the feasibility of using a pulsed deuterium-tritium (D-T) neutron technique for active interrogation of special nuclear material in cargo containers. Time distributions of fission neutrons from highly enriched uranium induced by a pulsed D-T neutron source were calculated for cargo containers with different hydrogen contents. A simple detector system with polyethylene and cadmium was modeled to calculate the two-group neutron flux at the detector.

  16. Modern approaches to cargo protection of rail transportation

    Directory of Open Access Journals (Sweden)

    Денис Викторович Ломотько

    2014-12-01

    Full Text Available On the basis of the study we have proposed a number of measures to improve the cargo protection during rail transportation. The economic impact of some of them is estimated. The legal relations of railways and cargo owners in terms of liability for failure to preserve are considered and proposed the management model of insurance risks of failure to preserve in transportation.

  17. Vibration and acoustic environments for payload/cargo integration

    Science.gov (United States)

    Hill, R. E.; Coody, M. C.

    1983-01-01

    Shuttle orbiter launch vibration and acoustic environments for cargo bay/payload interfaces are predicted. Data acquired during Shuttle flight tests are compared with the preflight estimates. Vibration response data for payload attachment locations are presented, along with acoustic data in the form of noise-level spectra measured at various locations in the cargo bay and space averages of the noise levels. It is shown that the payload-bay vibration and acoustic environments are generally less severe than predicted.

  18. Phototriggered cargo release from virus-like assemblies.

    Science.gov (United States)

    Brasch, Melanie; Voets, Ilja K; Koay, Melissa S T; Cornelissen, Jeroen J L M

    2013-01-01

    There has been tremendous progress towards the development of responsive polymers that are programmed to respond to an external stimulus such as light, pH and temperature. The unique combination of molecular packaging followed by slow, controlled release of molecular cargo is of particular importance for self-healing materials and the controlled release of drugs. While much focus and progress remains centred around synthetic carriers, viruses and virus-like particles can be considered ideal cargo carriers as they are intrinsically designed to package, protect and deliver nucleic acid cargo to host cells. Here, we report the encapsulation of a stimuli-responsive self-immolative polymer within virus-like assemblies of Cowpea Chlorotic Mottle Virus. Upon photo-irradiation, the self-immolative polymer undergoes a head-to-tail depolymerization into its monomeric subunits, resulting in the slow release of the molecular cargo. We propose that the liberated monomers are small enough to diffuse through the pores of the virus capsid shell and offer an alternative strategy for the controlled loading and unloading of the molecular cargo using viruses as cargo carriers.

  19. Integrated nanotechnology platform for tumor-targeted multimodal imaging and therapeutic cargo release.

    Science.gov (United States)

    Hosoya, Hitomi; Dobroff, Andrey S; Driessen, Wouter H P; Cristini, Vittorio; Brinker, Lina M; Staquicini, Fernanda I; Cardó-Vila, Marina; D'Angelo, Sara; Ferrara, Fortunato; Proneth, Bettina; Lin, Yu-Shen; Dunphy, Darren R; Dogra, Prashant; Melancon, Marites P; Stafford, R Jason; Miyazono, Kohei; Gelovani, Juri G; Kataoka, Kazunori; Brinker, C Jeffrey; Sidman, Richard L; Arap, Wadih; Pasqualini, Renata

    2016-02-16

    A major challenge of targeted molecular imaging and drug delivery in cancer is establishing a functional combination of ligand-directed cargo with a triggered release system. Here we develop a hydrogel-based nanotechnology platform that integrates tumor targeting, photon-to-heat conversion, and triggered drug delivery within a single nanostructure to enable multimodal imaging and controlled release of therapeutic cargo. In proof-of-concept experiments, we show a broad range of ligand peptide-based applications with phage particles, heat-sensitive liposomes, or mesoporous silica nanoparticles that self-assemble into a hydrogel for tumor-targeted drug delivery. Because nanoparticles pack densely within the nanocarrier, their surface plasmon resonance shifts to near-infrared, thereby enabling a laser-mediated photothermal mechanism of cargo release. We demonstrate both noninvasive imaging and targeted drug delivery in preclinical mouse models of breast and prostate cancer. Finally, we applied mathematical modeling to predict and confirm tumor targeting and drug delivery. These results are meaningful steps toward the design and initial translation of an enabling nanotechnology platform with potential for broad clinical applications.

  20. Stochastic simulations of cargo transport by processive molecular motors.

    Science.gov (United States)

    Korn, Christian B; Klumpp, Stefan; Lipowsky, Reinhard; Schwarz, Ulrich S

    2009-12-28

    We use stochastic computer simulations to study the transport of a spherical cargo particle along a microtubule-like track on a planar substrate by several kinesin-like processive motors. Our newly developed adhesive motor dynamics algorithm combines the numerical integration of a Langevin equation for the motion of a sphere with kinetic rules for the molecular motors. The Langevin part includes diffusive motion, the action of the pulling motors, and hydrodynamic interactions between sphere and wall. The kinetic rules for the motors include binding to and unbinding from the filament as well as active motor steps. We find that the simulated mean transport length increases exponentially with the number of bound motors, in good agreement with earlier results. The number of motors in binding range to the motor track fluctuates in time with a Poissonian distribution, both for springs and cables being used as models for the linker mechanics. Cooperativity in the sense of equal load sharing only occurs for high values for viscosity and attachment time.

  1. Cargo Transport by Two Coupled Myosin Va Motors on Actin Filaments and Bundles.

    Science.gov (United States)

    Ali, M Yusuf; Vilfan, Andrej; Trybus, Kathleen M; Warshaw, David M

    2016-11-15

    Myosin Va (myoVa) is a processive, actin-based molecular motor essential for intracellular cargo transport. When a cargo is transported by an ensemble of myoVa motors, each motor faces significant physical barriers and directional challenges created by the complex actin cytoskeleton, a network of actin filaments and actin bundles. The principles that govern the interaction of multiple motors attached to the same cargo are still poorly understood. To understand the mechanical interactions between multiple motors, we developed a simple in vitro model in which two individual myoVa motors labeled with different-colored Qdots are linked via a third Qdot that acts as a cargo. The velocity of this two-motor complex was reduced by 27% as compared to a single motor, whereas run length was increased by only 37%, much less than expected from multimotor transport models. Therefore, at low ATP, which allowed us to identify individual motor steps, we investigated the intermotor dynamics within the two-motor complex. The randomness of stepping leads to a buildup of tension in the linkage between motors-which in turn slows down the leading motor-and increases the frequency of backward steps and the detachment rate. We establish a direct relationship between the velocity reduction and the distribution of intermotor distances. The analysis of run lengths and dwell times for the two-motor complex, which has only one motor engaged with the actin track, reveals that half of the runs are terminated by almost simultaneous detachment of both motors. This finding challenges the assumptions of conventional multimotor models based on consecutive motor detachment. Similar, but even more drastic, results were observed with two-motor complexes on actin bundles, which showed a run length that was even shorter than that of a single motor.

  2. Differential cargo mobilisation within Weibel-Palade bodies after transient fusion with the plasma membrane.

    Directory of Open Access Journals (Sweden)

    Nikolai I Kiskin

    Full Text Available Inflammatory chemokines can be selectively released from Weibel-Palade bodies (WPBs during kiss-and-run exocytosis. Such selectivity may arise from molecular size filtering by the fusion pore, however differential intra-WPB cargo re-mobilisation following fusion-induced structural changes within the WPB may also contribute to this process. To determine whether WPB cargo molecules are differentially re-mobilised, we applied FRAP to residual post-fusion WPB structures formed after transient exocytosis in which some or all of the fluorescent cargo was retained. Transient fusion resulted in WPB collapse from a rod to a spheroid shape accompanied by substantial swelling (>2 times by surface area and membrane mixing between the WPB and plasma membranes. Post-fusion WPBs supported cumulative WPB exocytosis. To quantify diffusion inside rounded organelles we developed a method of FRAP analysis based on image moments. FRAP analysis showed that von Willebrand factor-EGFP (VWF-EGFP and the VWF-propolypeptide-EGFP (Pro-EGFP were immobile in post-fusion WPBs. Because Eotaxin-3-EGFP and ssEGFP (small soluble cargo proteins were largely depleted from post-fusion WPBs, we studied these molecules in cells preincubated in the weak base NH4Cl which caused WPB alkalinisation and rounding similar to that produced by plasma membrane fusion. In these cells we found a dramatic increase in mobilities of Eotaxin-3-EGFP and ssEGFP that exceeded the resolution of our method (∼ 2.4 µm2/s mean. In contrast, the membrane mobilities of EGFP-CD63 and EGFP-Rab27A in post-fusion WPBs were unchanged, while P-selectin-EGFP acquired mobility. Our data suggest that selective re-mobilisation of chemokines during transient fusion contributes to selective chemokine secretion during transient WPB exocytosis. Selective secretion provides a mechanism to regulate intravascular inflammatory processes with reduced risk of thrombosis.

  3. Differential cargo mobilisation within Weibel-Palade bodies after transient fusion with the plasma membrane.

    Science.gov (United States)

    Kiskin, Nikolai I; Babich, Victor; Knipe, Laura; Hannah, Matthew J; Carter, Tom

    2014-01-01

    Inflammatory chemokines can be selectively released from Weibel-Palade bodies (WPBs) during kiss-and-run exocytosis. Such selectivity may arise from molecular size filtering by the fusion pore, however differential intra-WPB cargo re-mobilisation following fusion-induced structural changes within the WPB may also contribute to this process. To determine whether WPB cargo molecules are differentially re-mobilised, we applied FRAP to residual post-fusion WPB structures formed after transient exocytosis in which some or all of the fluorescent cargo was retained. Transient fusion resulted in WPB collapse from a rod to a spheroid shape accompanied by substantial swelling (>2 times by surface area) and membrane mixing between the WPB and plasma membranes. Post-fusion WPBs supported cumulative WPB exocytosis. To quantify diffusion inside rounded organelles we developed a method of FRAP analysis based on image moments. FRAP analysis showed that von Willebrand factor-EGFP (VWF-EGFP) and the VWF-propolypeptide-EGFP (Pro-EGFP) were immobile in post-fusion WPBs. Because Eotaxin-3-EGFP and ssEGFP (small soluble cargo proteins) were largely depleted from post-fusion WPBs, we studied these molecules in cells preincubated in the weak base NH4Cl which caused WPB alkalinisation and rounding similar to that produced by plasma membrane fusion. In these cells we found a dramatic increase in mobilities of Eotaxin-3-EGFP and ssEGFP that exceeded the resolution of our method (∼ 2.4 µm2/s mean). In contrast, the membrane mobilities of EGFP-CD63 and EGFP-Rab27A in post-fusion WPBs were unchanged, while P-selectin-EGFP acquired mobility. Our data suggest that selective re-mobilisation of chemokines during transient fusion contributes to selective chemokine secretion during transient WPB exocytosis. Selective secretion provides a mechanism to regulate intravascular inflammatory processes with reduced risk of thrombosis.

  4. Cargo proteins of plasma astrocyte-derived exosomes in Alzheimer's disease.

    Science.gov (United States)

    Goetzl, Edward J; Mustapic, Maja; Kapogiannis, Dimitrios; Eitan, Erez; Lobach, Irina V; Goetzl, Laura; Schwartz, Janice B; Miller, Bruce L

    2016-11-01

    Efficient intercellular transfer of RNAs, proteins, and lipids as protected exosomal cargo has been demonstrated in the CNS, but distinct physiologic and pathologic roles have not been well defined for this pathway. The capacity to isolate immunochemically human plasma neuron-derived exosomes (NDEs), containing neuron-specific cargo, has permitted characterization of CNS-derived exosomes in living humans. Constituents of the amyloid β-peptide (Aβ)42-generating system now are examined in 2 distinct sets of human neural cells by quantification in astrocyte-derived exosomes (ADEs) and NDEs, enriched separately from plasmas of patients with Alzheimer's disease (AD) or frontotemporal dementia (FTD) and matched cognitively normal controls. ADE levels of β-site amyloid precursor protein-cleaving enzyme 1 (BACE-1), γ-secretase, soluble Aβ42, soluble amyloid precursor protein (sAPP)β, sAPPα, glial-derived neurotrophic factor (GDNF), P-T181-tau, and P-S396-tau were significantly (3- to 20-fold) higher than levels in NDEs for patients and controls. BACE-1 levels also were a mean of 7-fold higher in ADEs than in NDEs from cultured rat type-specific neural cells. Levels of BACE-1 and sAPPβ were significantly higher and of GDNF significantly lower in ADEs of patients with AD than in those of controls, but not significantly different in patients with FTD than in controls. Abundant proteins of the Aβ42 peptide-generating system in ADEs may sustain levels in neurons. ADE cargo proteins may be useful for studies of mechanisms of cellular interactions and effects of BACE-1 inhibitors in AD.-Goetzl, E. J., Mustapic, M., Kapogiannis, D., Eitan, E., Lobach, I. V., Goetzl, L., Schwartz, J. B., Miller, B. L. Cargo proteins of plasma astrocyte-derived exosomes in Alzheimer's disease.

  5. Rapid response calculation of LNG cargo containment system under sloshing load using wavelet transformation

    Science.gov (United States)

    Kim, Yooil

    2013-06-01

    Reliable strength assessment of the Liquefied Natural Gas (LNG) cargo containment system under the sloshing impact load is very difficult task due to the complexity of the physics involved in, both in terms of the hydrodynamics and structural mechanics. Out of all those complexities, the proper selection of the design sloshing load which is applied to the structural model of the LNG cargo containment system, is one of the most challenging one due to its inherent randomness as well as the statistical analysis which is tightly linked to the design sloshing load selection. In this study, the response based strength assessment procedure of LNG cargo containment system has been developed and proposed as an alternative design methodology. Sloshing pressure time history, measured from the model test, is decomposed into wavelet basis function targeting the minimization of the number of the basis function together with the maximization of the numerical efficiency. Then the response of the structure is obtained using the finite element method under each wavelet basis function of different scale. Finally, the response of the structure under entire sloshing impact time history is rapidly calculated by synthesizing the structural response under wavelet basis function. Through this analysis, more realistic response of the system under sloshing impact pressure can be obtained without missing the details of pressure time history such as rising pattern, oscillation due to air entrapment and decay pattern and so on. The strength assessment of the cargo containment system is then performed based on the statistical analysis of the stress peaks selected out of the obtained stress time history.

  6. Comparative International Air Cargo Solutions: The Pathway to a Resilient, Adaptable, Balanced and Sustainable Secure Global Air Cargo Supply Chain

    Science.gov (United States)

    2013-12-01

    systems. Dr. Gerald L. Dillinham, the Director of Physical Infrastructure Issues, GAO noted during his testimony that securing the nation’s aviation...9697. Karp , Aaron. “Air Cargo Flattens Out.” Air Transport World 46, no. 1 (2009): 37–39. 74 Kersey, J. W. “The Economics of Air Cargo (Analysis of...Transportation, U.S. Senate. Aviation Security: Progress since September 11, 2001, and the Challenges Ahead. Statement of Gerald L. Dillingham

  7. Spectroscopic neutron radiography for a cargo scanning system

    Science.gov (United States)

    Rahon, Jill; Danagoulian, Areg; MacDonald, Thomas D.; Hartwig, Zachary S.; Lanza, Richard C.

    2016-06-01

    Detection of cross-border smuggling of illicit materials and contraband is a challenge that requires rapid, low-dose, and efficient radiographic technology. The work we describe here is derived from a technique which uses monoenergetic gamma rays from low energy nuclear reactions, such as 11B(d,nγ)12C, to perform radiographic analysis of shipping containers. Transmission ratios of multiple monoenergetic gamma lines resulting from several gamma producing nuclear reactions can be employed to detect materials of high atomic number (Z), the details of which will be described in a separate paper. Inherent in this particular nuclear reaction is the production of fast neutrons which could enable neutron radiography and further characterization of the effective-Z of the cargo, especially within the range of lower Z. Previous research efforts focused on the use of total neutron counts in combination with X-ray radiography to characterize the hydrogenous content of the cargo. We present a technique of performing transmitted neutron spectral analysis to reconstruct the effective Z and potentially the density of the cargo. This is made possible by the large differences in the energy dependence of neutron scattering cross-sections between hydrogenous materials and those of higher Z. These dependencies result in harder transmission spectra for hydrogenous cargoes than those of non-hydrogenous cargoes. Such observed differences can then be used to classify the cargo based on its hydrogenous content. The studies presented in this paper demonstrate that such techniques are feasible and can provide a contribution to cargo security, especially when used in concert with gamma radiography.

  8. Spectroscopic neutron radiography for a cargo scanning system

    Energy Technology Data Exchange (ETDEWEB)

    Rahon, Jill [Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States); Danagoulian, Areg, E-mail: aregjan@mit.edu [Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States); MacDonald, Thomas D. [Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States); Hartwig, Zachary S. [Plasma Science and Fusion Center, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States); Lanza, Richard C. [Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States)

    2016-06-01

    Detection of cross-border smuggling of illicit materials and contraband is a challenge that requires rapid, low-dose, and efficient radiographic technology. The work we describe here is derived from a technique which uses monoenergetic gamma rays from low energy nuclear reactions, such as {sup 11}B(d,nγ){sup 12}C, to perform radiographic analysis of shipping containers. Transmission ratios of multiple monoenergetic gamma lines resulting from several gamma producing nuclear reactions can be employed to detect materials of high atomic number (Z), the details of which will be described in a separate paper. Inherent in this particular nuclear reaction is the production of fast neutrons which could enable neutron radiography and further characterization of the effective-Z of the cargo, especially within the range of lower Z. Previous research efforts focused on the use of total neutron counts in combination with X-ray radiography to characterize the hydrogenous content of the cargo. We present a technique of performing transmitted neutron spectral analysis to reconstruct the effective Z and potentially the density of the cargo. This is made possible by the large differences in the energy dependence of neutron scattering cross-sections between hydrogenous materials and those of higher Z. These dependencies result in harder transmission spectra for hydrogenous cargoes than those of non-hydrogenous cargoes. Such observed differences can then be used to classify the cargo based on its hydrogenous content. The studies presented in this paper demonstrate that such techniques are feasible and can provide a contribution to cargo security, especially when used in concert with gamma radiography.

  9. Project CarGO tram at Dresden; Projekt CarGo Tram in Dresden

    Energy Technology Data Exchange (ETDEWEB)

    Mueller-Eberstein, F.; Franke, M. [Dresdner Verkehrsbetriebe AG, Dresden (Germany)

    2000-05-01

    Next year, the Glassen Volkswagen Factory, which is currently under construction, will commence the production of luxury class automobiles. Already buying a car is meant to be a special event; thus the factory is set in a baroque environment, near the famous Semper Opera and Picture Gallery. This arrangement, hitherto without example, will be accompanied by another, equally remarkable project. The factory, which is situated in a central city area being very sensitive in terms of urban development and traffic installations, will be delivered raw materials and parts using a CarGo Tram. Two bi-directionally operated, 60 metres long five-car trains will transport the parts from the railway cargo hub at Dresden Friedrichstadt to the factory in cycles of 40 minutes. There and at the Glassen Factory in Grosser Garten, separate track terminals will be built. In addition to the historic past and the charming scenery of the Elbe metropolis Dresden and its environs, soon also the CarGo Tram and the Glassen Factory will attract guests from all over the world to visit this innovative and culturally important city in the centre of Europe. (orig.) [German] Im Jahr 2001 beginnt die Produktion von Automobilen der Oberklasse in der derzeit noch im Bau befindlichen Glaesernen Manufaktur der Volkswagen Automobil-Manufaktur Dresden GmbH. In einem barocken Umfeld, mit raeumlicher Naehe zur Semperoper und Gemaeldegalerie, soll bereits der Verkauf dieser Fahrzeuge zu einem Erlebnis werden. Dieses bisher einmalige Vorhaben wird von einem zweiten, ebenso einzigartigen Vorhaben begleitet. Die Belieferung der in einem staedtebaulich und verkehrlich sensiblen Innenstadtbereich gelegenen Manufaktur erfolgt mit einer Gueterstrassenbahn. Zwei 60 Meter lange, fuenfteilige Zweirichtungsfahrzeuge werden ab Maerz 2001 etwa im 40-Minutentakt die Anlieferung der Zulieferteile aus dem Gueterverkehrszentrum Dresden-Friedrichstadt uebernehmen. Hier und an der Glaesernen Manufaktur im Grossen Garten werden

  10. Stokes Efficiency of Molecular Motor-Cargo Systems

    Directory of Open Access Journals (Sweden)

    Hongyun Wang

    2008-01-01

    Full Text Available A molecular motor utilizes chemical free energy to generate a unidirectional motion through the viscous fluid. In many experimental settings and biological settings, a molecular motor is elastically linked to a cargo. The stochastic motion of a molecular motor-cargo system is governed by a set of Langevin equations, each corresponding to an individual chemical occupancy state. The change of chemical occupancy state is modeled by a continuous time discrete space Markov process. The probability density of a motor-cargo system is governed by a two-dimensional Fokker-Planck equation. The operation of a molecular motor is dominated by high viscous friction and large thermal fluctuations from surrounding fluid. The instantaneous velocity of a molecular motor is highly stochastic: the past velocity is quickly damped by the viscous friction and the new velocity is quickly excited by bombardments of surrounding fluid molecules. Thus, the theory for macroscopic motors should not be applied directly to molecular motors without close examination. In particular, a molecular motor behaves differently working against a viscous drag than working against a conservative force. The Stokes efficiency was introduced to measure how efficiently a motor uses chemical free energy to drive against viscous drag. For a motor without cargo, it was proved that the Stokes efficiency is bounded by 100% [H. Wang and G. Oster, (2002]. Here, we present a proof for the general motor-cargo system.

  11. Optimisation of key performance measures in air cargo demand management

    Directory of Open Access Journals (Sweden)

    Alexander May

    2014-03-01

    Full Text Available This article sought to facilitate the optimisation of key performance measures utilised for demand management in air cargo operations. The focus was on the Revenue Management team at Virgin Atlantic Cargo and a fuzzy group decision-making method was used. Utilising intelligent fuzzy multi-criteria methods, the authors generated a ranking order of ten key outcome-based performance indicators for Virgin Atlantic air cargo Revenue Management. The result of this industry-driven study showed that for Air Cargo Revenue Management, ‘Network Optimisation’ represents a critical outcome-based performance indicator. This collaborative study contributes to existing logistics management literature, especially in the area of Revenue Management, and it seeks to enhance Revenue Management practice. It also provides a platform for Air Cargo operators seeking to improve reliability values for their key performance indicators as a means of enhancing operational monitoring power.

  12. Reducing Mission Logistics with Multipurpose Cargo Transfer Bags

    Science.gov (United States)

    Baccus, Shelley; Broyan, James Lee, Jr.; Borrego, Melissa

    2016-01-01

    The Logistics Reduction (LR) project within Advanced Exploration Systems (AES) is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag (CTB), the common logistics carrying bag for the International Space Station (ISS). After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unfolded into a flat panel for reuse. Concepts and potential benefits for various MCTB applications will be discussed including partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing. Acoustic MCTBs are currently in use on ISS to reduce the noise generated by the T2 treadmill, which reaches the hazard limit at high speeds. The development of the AMCTB included identification of keep-out zones, acoustic properties, deployment considerations, and structural testing. Features developed for these considerations are applicable to MCTBs for all crew outfitting applications.

  13. Multipurpose Cargo Transfer Bags fro Reducing Exploration Mission Logistics

    Science.gov (United States)

    Baccus, Shelley; Broyan, James Lee, Jr.; Borrego, Melissa

    2016-01-01

    The Logistics Reduction (LR) project within the Advanced Exploration Systems (AES) division is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag (CTB), the common logistics carrying bag for the International Space Station (ISS). After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unfolded into a flat panel for reuse. Concepts and potential benefits for various MCTB applications will be discussed including partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing. Acoustic MCTBs are currently in use on ISS to reduce the noise generated by the T2 treadmill, which reaches the hazard limit at high speeds. The development of the AMCTB included identification of keep out zones, acoustic properties, deployment considerations, and structural testing. Features developed for these considerations are applicable to MCTBs for all crew outfitting applications.

  14. Shared Value Potential of Transporting Cargo via Hyperloop

    Directory of Open Access Journals (Sweden)

    Max Werner

    2016-08-01

    Full Text Available This research estimates the shared value created by constructing a hypothetical Hyperloop to transport cargo along 300 km in Northern Germany. Following Porter-Kramer (2011, we identified and evaluated eight factors that create shared value: travel speed, operating costs, safety, noise pollution, air pollution, climate effect/carbon footprint, separation effect/ property efficiency, and maintenance. Using official data compiled by several German institutes and organizations, we conducted comparative analysis to quantify and compare the abovementioned factors for Hyperloop and over-the-road cargo transport in Germany. Then, we monetized the individual and collective benefits of the shared value created by Hyperloop replacing a significant share of cargo transported by truck. Our findings indicate that the hypothetical Hyperloop project in Northern Germany would create €660 to €900 million of shared value annually. Our research method establishes a framework for assessing future transportation projects like Hyperloop, and our findings can be generalized to industrialized nations beyond Germany.

  15. Coupling of Active Motion and Advection Shapes Intracellular Cargo Transport

    CERN Document Server

    Trong, P Khuc; Goldstein, R E; 10.1103/PhysRevLett.109.028104

    2012-01-01

    Intracellular cargo transport can arise from passive diffusion, active motor-driven transport along cytoskeletal filament networks, and passive advection by fluid flows entrained by such motor/cargo motion. Active and advective transport are thus intrinsically coupled as related, yet different representations of the same underlying network structure. A reaction-advection-diffusion system is used here to show that this coupling affects the transport and localization of a passive tracer in a confined geometry. For sufficiently low diffusion, cargo localization to a target zone is optimized either by low reaction kinetics and decoupling of bound and unbound states, or by a mostly disordered cytoskeletal network with only weak directional bias. These generic results may help to rationalize subtle features of cytoskeletal networks, for example as observed for microtubules in fly oocytes.

  16. Optimizing an undulating magnetic microswimmer for cargo towing

    Science.gov (United States)

    Gutman, Emiliya; Or, Yizhar

    2016-06-01

    One of the promising capabilities of magnetic microswimmers is towing a cargo, which can be used for targeted drug delivery or performing tissue biopsy. A key question is what should be the optimal size ratio between the cargo and the swimmer's flexible tail. This question is addressed here for the simplest theoretical model of a magnetic microswimmer undergoing planar undulations—a spherical load connected by a torsion spring to a rigid slender link. The swimmer's dynamic is formulated and leading-order expressions for its motion are obtained explicitly under small-amplitude approximation. Optimal combinations of magnetic actuation frequency, torsion stiffness, and tail length for maximizing displacement, average speed, or energetic efficiency are obtained. The theoretical results are compared with reported experiments in several types of cargo-towing magnetic microswimmers.

  17. 75 FR 63192 - Intent To Request Renewal From OMB of One Current Public Collection of Information: Air Cargo...

    Science.gov (United States)

    2010-10-14

    ... Collection of Information: Air Cargo Security Requirements AGENCY: Transportation Security Administration... under a security program, and all-cargo carriers: Security programs, security threat assessments (STA), known shipper data via the Known Shipper Management System (KSMS), cargo screening reporting,...

  18. Solving the Liner Shipping Fleet Repositioning Problem with Cargo Flows

    DEFF Research Database (Denmark)

    Tierney, Kevin; Askelsdottir, Björg; Jensen, Rune Møller;

    2015-01-01

    to reposition vessels as cheaply as possible without disrupting cargo flows. The LSFRP is characterized by chains of interacting activities with a multicommodity flow over paths defined by the activities chosen. Despite its industrial importance, the LSFRP has received little attention in the literature. We...... introduce a novel mathematical model and a simulated annealing algorithm for the LSFRP with cargo flows that makes use of a carefully constructed graph; we evaluate these approaches using real-world data from our industrial collaborator. Additionally, we compare the performance of our approach against...

  19. A matheuristic for the Cargo Mix Problem with Block Stowage

    DEFF Research Database (Denmark)

    Christensen, Jonas Mark; Pacino, Dario

    2017-01-01

    The cargo-mix problem aims at selecting the amount of containers of a given type to load on a vessel. In this article we present an extended definition that includes the analysis of a circular route with draft restrictions, limitations on expected cargo and the use of a block stowage strategy. A ....... A compact formulation of the problem based on the state-of-the-art heuristic decomposition is shown not to be able to solve the extended problem, thus a matheuristic approach is presented that can achieve high quality results in a matter of seconds....

  20. Column generation approaches to ship scheduling with flexible cargo sizes

    DEFF Research Database (Denmark)

    Brønmo, Geir; Nygreen, Bjørn; Lysgaard, Jens

    cargo sizes to the column generation framework is not straightforward, and we handle the flexible cargo sizes heuristically when solving the subproblems. This leads to convergence issues in the branch-and-price search tree, and the optimal solution cannot be guaranteed. Hence we have introduced a method...... that generates an upper bound on the optimal objective. We have compared our method with an a priori column generation approach, and our computational experiments on real world cases show that the Dantzig-Wolfe approach is faster than the a priori generation of columns, and we are able to deal with larger...

  1. REEPs are membrane shaping adapter proteins that modulate specific g protein-coupled receptor trafficking by affecting ER cargo capacity.

    Science.gov (United States)

    Björk, Susann; Hurt, Carl M; Ho, Vincent K; Angelotti, Timothy

    2013-01-01

    Receptor expression enhancing proteins (REEPs) were identified by their ability to enhance cell surface expression of a subset of G protein-coupled receptors (GPCRs), specifically GPCRs that have proven difficult to express in heterologous cell systems. Further analysis revealed that they belong to the Yip (Ypt-interacting protein) family and that some REEP subtypes affect ER structure. Yip family comparisons have established other potential roles for REEPs, including regulation of ER-Golgi transport and processing/neuronal localization of cargo proteins. However, these other potential REEP functions and the mechanism by which they selectively enhance GPCR cell surface expression have not been clarified. By utilizing several REEP family members (REEP1, REEP2, and REEP6) and model GPCRs (α2A and α2C adrenergic receptors), we examined REEP regulation of GPCR plasma membrane expression, intracellular processing, and trafficking. Using a combination of immunolocalization and biochemical methods, we demonstrated that this REEP subset is localized primarily to ER, but not plasma membranes. Single cell analysis demonstrated that these REEPs do not specifically enhance surface expression of all GPCRs, but affect ER cargo capacity of specific GPCRs and thus their surface expression. REEP co-expression with α2 adrenergic receptors (ARs) revealed that this REEP subset interacts with and alter glycosidic processing of α2C, but not α2A ARs, demonstrating selective interaction with cargo proteins. Specifically, these REEPs enhanced expression of and interacted with minimally/non-glycosylated forms of α2C ARs. Most importantly, expression of a mutant REEP1 allele (hereditary spastic paraplegia SPG31) lacking the carboxyl terminus led to loss of this interaction. Thus specific REEP isoforms have additional intracellular functions besides altering ER structure, such as enhancing ER cargo capacity, regulating ER-Golgi processing, and interacting with select cargo proteins

  2. 49 CFR 178.347 - Specification DOT 407; cargo tank motor vehicle.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 407; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.347 Specification DOT 407; cargo tank motor vehicle....

  3. 49 CFR 178.346 - Specification DOT 406; cargo tank motor vehicle.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 406; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.346 Specification DOT 406; cargo tank motor vehicle....

  4. 49 CFR 178.348 - Specification DOT 412; cargo tank motor vehicle.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 412; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.348 Specification DOT 412; cargo tank motor vehicle....

  5. Minimization of Load Oscillations in Travelling Mechanisms of Cranes

    Directory of Open Access Journals (Sweden)

    Kamal Khandakji

    2005-01-01

    Full Text Available The paper presents an analysis of suspended load (cargo oscillations in traveling mechanisms of cranes. A special control algorithm has been developed in order to get minimum cargo oscillations in minimum transient time. Mathematical model simulating the effect of the suggested control algorithm on cargo oscillations has been developed. Two power electronic circuits applicable for AC drives with three-phase induction motors are suggested to obtain the required motion algorithm.

  6. 中国-东盟自由贸易区大部分货物贸易2010年零关税%Zero tariff expected for most cargo trade in China-ASEAN free trade zone by 2010

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The negotiation committees of China and the Association of Southeast Asian Nations (ASEAN) have reached agreement on the most important prat-agreement on cargo trade, according to the Ministry of Commerce spokesman Chong Quan. The two sides decided to sign two agreements on cargo trade and dispute resolution mechanism of the China-ASEAN free trade zone duringthe China-ASEAN leaders' meeting in November 2004.

  7. 19 CFR 192.14 - Electronic information for outward cargo required in advance of departure.

    Science.gov (United States)

    2010-04-01

    ... vessel cargo is to be laden; (ii) For air cargo, including cargo being transported by Air Express.... The AES Commodity Module already captures the requisite export data, so no new data elements for...-ment (low-risk exporter or other exemption) for any export shipment laden that is not subject to...

  8. 29 CFR 1918.83 - Stowed cargo; tiering and breaking down.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Stowed cargo; tiering and breaking down. 1918.83 Section... cargo; tiering and breaking down. (a) When necessary to protect personnel working in a hold, the employer shall secure or block stowed cargo that is likely to shift or roll. (b) In breaking down...

  9. 77 FR 21577 - Agency Information Collection Activities: Cargo Container and Road Vehicle Certification for...

    Science.gov (United States)

    2012-04-10

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Cargo Container and... collection requirement concerning the Cargo Container and Road Vehicle for Transport under Customs Seal. This...: Title: Cargo Container and Road Vehicle for Transport under Customs Seal. OMB Number: 1651-0124....

  10. 46 CFR 153.1000 - Special operating requirements for cargoes reactive with water.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Special operating requirements for cargoes reactive with water. 153.1000 Section 153.1000 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED... MATERIALS Operations Special Cargo Procedures § 153.1000 Special operating requirements for cargoes...

  11. 46 CFR 153.935 - Opening of tanks and cargo sampling.

    Science.gov (United States)

    2010-10-01

    ..., except: (1) To clean a tank; (2) To transfer a cargo that Table 1 allows in a containment system having... 46 Shipping 5 2010-10-01 2010-10-01 false Opening of tanks and cargo sampling. 153.935 Section 153... Vessel Safety § 153.935 Opening of tanks and cargo sampling. (a) Except as provided in paragraph (b)...

  12. 33 CFR 126.27 - General permit for handling dangerous cargo.

    Science.gov (United States)

    2010-07-01

    ...) in bulk, portable tanks, containers, or packagings, at designated waterfront facilities, conditioned... cargo in limited-quantity packaging. (e) Transport units and portable tanks containing dangerous cargo... in the event of an accident. Cargo not required to be segregated, when in break-bulk form, is...

  13. Safety evaluation for packaging transport of LSA-II liquids in MC-312 cargo tanks

    Energy Technology Data Exchange (ETDEWEB)

    Carlstrom, R.F.

    1996-09-11

    This safety evaluation for packaging authorizes the onsite transfer of bulk LSA-II radioactive liquids in the 222-S Laboratory Cargo Tank and Liquid Effluent Treatment Facility Cargo Tanks (which are U.S. Department of Transportation MC-312 specification cargo tanks) from their operating facilities to tank farm facilities.

  14. 46 CFR 308.521 - Application for Open Cargo Policy, Form MA-301.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Application for Open Cargo Policy, Form MA-301. 308.521 Section 308.521 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR... for Open Cargo Policy, Form MA-301. The standard form of application for a War Risk Open Cargo...

  15. 46 CFR 154.650 - Cargo tank and process pressure vessel welding.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank and process pressure vessel welding. 154.650... Equipment Construction § 154.650 Cargo tank and process pressure vessel welding. (a) Cargo tank and process pressure vessel welding must meet Subpart 54.05 and Part 57 of this chapter. (b) Welding consumables...

  16. 76 FR 4847 - Hazardous Materials: Safety Requirements for External Product Piping on Cargo Tanks Transporting...

    Science.gov (United States)

    2011-01-27

    ...: Safety Requirements for External Product Piping on Cargo Tanks Transporting Flammable Liquids AGENCY... flammable liquids in unprotected external product piping on DOT specification cargo tank motor vehicles. If... specification cargo tank motor vehicle (CTMV), unless the vehicle is equipped with bottom damage...

  17. 77 FR 3487 - Agency Information Collection Activities: Transfer of Cargo to a Container Station

    Science.gov (United States)

    2012-01-24

    ... Information Collection Activities: Transfer of Cargo to a Container Station AGENCY: U.S. Customs and Border... information collection requirement concerning Transfer of Cargo to a Container Station. This request for...: Title: Transfer of Cargo to a Container Station. OMB Number: 1651-0096. Form Number: None....

  18. 49 CFR 173.318 - Cryogenic liquids in cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... temperature (one pound of water=27.737 cubic inches at 60 °F., or one gallon of water = 231 cubic inches at 60... density following partial unloading. Multiple OWTT markings for different pressure levels are permitted... the design service temperature of the packaging. (2) A cargo tank may not be loaded with any...

  19. 19 CFR 4.62 - Accounting for inward cargo.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Accounting for inward cargo. 4.62 Section 4.62 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY VESSELS IN FOREIGN AND DOMESTIC TRADES Foreign Clearances § 4.62 Accounting for inward...

  20. 29 CFR 1918.85 - Containerized cargo operations.

    Science.gov (United States)

    2010-07-01

    ... hookup, anchoring and tie-off techniques, methods of use, and proper methods of equipment inspection and... such container is empty. Methods of identification may include cargo plans, manifests, or markings on... the method of calculation described in paragraph (b)(4)(ii) of this section or by shipping...

  1. Bicaudal-D: Switching motors, cargo and direction

    NARCIS (Netherlands)

    G.D. Splinter (Daniël)

    2008-01-01

    textabstractScope of this thesis Transport of vesicles and organelles is an essential cellular process. Proteins like Rab GTPases, specialized adaptor proteins and motor proteins are involved in targeting and movement of cargos to their destination. This thesis describes the function of the mammalia

  2. 33 CFR 157.132 - Cargo tanks: Hydrocarbon vapor emissions.

    Science.gov (United States)

    2010-07-01

    ...(a)(2) or § 157.10c(b)(2) without sufficient segregated ballast tanks or dedicated clean ballast... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Cargo tanks: Hydrocarbon vapor... SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK...

  3. 33 CFR 157.35 - Ballast added to cargo tanks.

    Science.gov (United States)

    2010-07-01

    ... segregated ballast tanks or dedicated clean ballast tanks under § 157.09, § 157.10, § 157.10a(a)(1), § 157...; (b) More ballast water than can be carried in segregated ballast tanks or dedicated clean ballast... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Ballast added to cargo tanks....

  4. 77 FR 65006 - Air Cargo Advance Screening (ACAS) Pilot Program

    Science.gov (United States)

    2012-10-24

    ..., such as the ACAS pilot, in 19 CFR 101.9. Advance Electronic Air Cargo Information Required by 19 CFR... need to sign an Interconnection Security Agreement (ISA) or amend their existing ISA, if necessary, and... connection covered by a valid and up to date ISA will have already met these requirements. ACAS...

  5. Shift designs for freight handling personnel at air cargo terminals

    DEFF Research Database (Denmark)

    Rong, Aiying; Grunow, Martin

    2009-01-01

    This paper presents an integrated mixed integer linear programming (MILP) model for determining manpower requirements and related personnel shift designs for the build-up and break-down of the unit load devices (ULDs) at the air cargo terminal to minimize manpower costs. To utilize the manpower...

  6. Screening Cargo Containers to Remove a Terrorist Threat

    Energy Technology Data Exchange (ETDEWEB)

    Hazi, A

    2005-09-20

    Each year some 48 million cargo containers move between the world's ports. More than 6 million of these enter the U.S., but only about 2 percent are opened and inspected when they arrive at U.S. seaports. The West Coast ports of Los Angeles-Long Beach, Oakland, and Seattle alone process 11,000 containers per day, or about 8 containers per minute. Because of this high traffic volume, U.S. seaports are especially vulnerable to a terrorist attack. Illicit radioactive materials could be hidden in any one of the cargo-filled containers that arrive at U.S. ports. Yet, searching every shipment would be bring legitimate commercial activities to a halt. Improving security at U.S. ports is thus one of the nation's most difficult technical and practical challenges because the systems developed for screening cargo must operate in concert with ongoing seaport activities. Working at this intersection of commerce and national security, Lawrence Livermore researchers are applying their expertise in radiation science and detection to develop improved technologies for detecting hidden radioactive materials. One new technology being designed and tested at the Laboratory is a neutron interrogation system for cargo containers. This system will quickly screen incoming shipments to ensure that nuclear materials such as plutonium and highly enriched uranium (HEU) are not smuggled into the U.S.

  7. 49 CFR 1548.9 - Acceptance of cargo.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Acceptance of cargo. 1548.9 Section 1548.9..., DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY INDIRECT AIR CARRIER SECURITY § 1548.9 Acceptance of... program. (b) Refusal to transport. Each indirect air carrier must refuse to offer for transport on...

  8. A consolidation model for cargo-driven intermodal transportation

    NARCIS (Netherlands)

    Behdani, B.; Zuidwijk, R.A.

    2014-01-01

    Accessibility of main sea ports by road has become increasingly difficult due to rapid growth in container throughput. To deal with this challenge, new ways for handling containers and distribution of cargo flows are needed to define and implement. One possibility is going beyond container transport

  9. Integrated cargo routing and ship scheduling in liner shipping

    DEFF Research Database (Denmark)

    Kjeldsen, Karina Hjortshøj

    The problem consists of creating routes and schedules for a heterogeneous fleet of ships while determining the cargo routing and the speed for all relevant port pair/ship combinations. Transshipment is allowed in ports with transshipment capabilities. The service frequency is fixed at one week...

  10. 77 FR 65395 - Air Cargo Advance Screening (ACAS) Pilot Program

    Science.gov (United States)

    2012-10-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Customs and Border Protection Air Cargo Advance Screening (ACAS) Pilot Program Correction In notice document 2012-26031 appearing on pages 65006-65009 in the issue of October 24, 2012 make...

  11. 19 CFR 122.48 - Air cargo manifest.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Air cargo manifest. 122.48 Section 122.48 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Entry and Entry Documents; Electronic Manifest Requirements...

  12. CH-47F Improved Cargo Helicopter (CH-47F)

    Science.gov (United States)

    2015-12-01

    supply chain and industrial base. Nuclear Costs None CH-47F December 2015 SAR March 21, 2016 18:18:21 UNCLASSIFIED 22 Unit Cost Unit Cost Report...mission is transportation of ground forces, Class III/Class V supplies , and other battle critical cargo in support of all future contingencies. The CH

  13. 49 CFR 175.75 - Quantity limitations and cargo location.

    Science.gov (United States)

    2010-10-01

    ... CFR 25.857(b) or for a Class C aircraft cargo compartment in 14 CFR 25.857(c). A package bearing a KEEP AWAY FROM HEAT handling marking must be protected from direct sunshine and stored in a cool and ventilated place, away from sources of heat. (c) For each package containing a hazardous material...

  14. Mars Hybrid Propulsion System Trajectory Analysis. Part II; Cargo Missions

    Science.gov (United States)

    Chai, Patrick R.; Merrill, Raymond G.; Qu, Min

    2015-01-01

    NASA's Human Spaceflight Architecture Team is developing a reusable hybrid transportation architecture in which both chemical and electric propulsion systems are used to send crew and cargo to Mars destinations such as Phobos, Deimos, the surface of Mars, and other orbits around Mars. By combining chemical and electrical propulsion into a single spaceship and applying each where it is more effective, the hybrid architecture enables a series of Mars trajectories that are more fuel-efficient than an all chemical architecture without significant increases in flight times. This paper shows the feasibility of the hybrid transportation architecture to pre-deploy cargo to Mars and Phobos in support of the Evolvable Mars Campaign crew missions. The analysis shows that the hybrid propulsion stage is able to deliver all of the current manifested payload to Phobos and Mars through the first three crew missions. The conjunction class trajectory also allows the hybrid propulsion stage to return to Earth in a timely fashion so it can be reused for additional cargo deployment. The 1,100 days total trip time allows the hybrid propulsion stage to deliver cargo to Mars every other Earth-Mars transit opportunity. For the first two Mars surface mission in the Evolvable Mars Campaign, the short trip time allows the hybrid propulsion stage to be reused for three round-trip journeys to Mars, which matches the hybrid propulsion stage's designed lifetime for three round-trip crew missions to the Martian sphere of influence.

  15. The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo

    Directory of Open Access Journals (Sweden)

    Jeoffrey Schageman

    2013-01-01

    Full Text Available Exosomes are small (30–150 nm vesicles containing unique RNA and protein cargo, secreted by all cell types in culture. They are also found in abundance in body fluids including blood, saliva, and urine. At the moment, the mechanism of exosome formation, the makeup of the cargo, biological pathways, and resulting functions are incompletely understood. One of their most intriguing roles is intercellular communication—exosomes function as the messengers, delivering various effector or signaling macromolecules between specific cells. There is an exponentially growing need to dissect structure and the function of exosomes and utilize them for development of minimally invasive diagnostics and therapeutics. Critical to further our understanding of exosomes is the development of reagents, tools, and protocols for their isolation, characterization, and analysis of their RNA and protein contents. Here we describe a complete exosome workflow solution, starting from fast and efficient extraction of exosomes from cell culture media and serum to isolation of RNA followed by characterization of exosomal RNA content using qRT-PCR and next-generation sequencing techniques. Effectiveness of this workflow is exemplified by analysis of the RNA content of exosomes derived from HeLa cell culture media and human serum, using Ion Torrent PGM as a sequencing platform.

  16. Advanced propulsion options for the Mars cargo mission

    Science.gov (United States)

    Frisbee, Robert H.; Blandino, John J.; Sercel, Joel C.; Sargent, Mark S.; Gowda, Nandini

    1990-01-01

    Several advanced propulsion options for a split-mission piloted Mars exploration scenario are presented. The primary study focus is on identifying concepts that can reduce total initial mass in low earth orbit (IMLEO) for the cargo delivery portion of the mission; in addition, concepts that can reduce the trip time of the piloted option are assessed. The propulsion options considered are nuclear thermal propulsion, solar sails, multimegawatt-class nuclear electric propulsion, solar electric propulsion, magnetic sails, mass drivers, rail guns, solar thermal rockets, beamed-energy propulsion systems, and tethers. For the cargo mission, solar sails are found to provide the greatest mass savings over the baseline chemical system, although they suffer from having very long trip times; a good performance compromise between a low IMLEO and a short trip time can be obtained using multimegawatt-class nuclear electric propulsion systems.

  17. Implementation of cargo MagLev in the United States

    Energy Technology Data Exchange (ETDEWEB)

    Rose, Chris R [Los Alamos National Laboratory; Peterson, Dean E [Los Alamos National Laboratory; Leung, Eddie M [MAGTEC ENGINEERING

    2008-01-01

    Numerous studies have been completed in the United States, but no commercial MagLev systems have been deployed. Outside the U.S., MagLev continues to attract funding for research, development and implementation. A brief review of recent global developments in MagLev technology is given followed by the status of MagLev in the U.S. The paper compares the cost of existing MagLev systems with other modes of transport, notes that the near-term focus of MagLev development in the U.S. should be for cargo, and suggests that future MagLev systems should be for very high speed cargo. The Los Angeles to Port of Los Angeles corridor is suggested as a first site for implementation. The benefits of MagLev are described along with suggestions on how to obtain funding.

  18. Development of a Cargo Screening Process Simulator: A First Approach

    CERN Document Server

    Siebers, Peer-Olaf; Aickelin, Uwe

    2010-01-01

    The efficiency of current cargo screening processes at sea and air ports is largely unknown as few benchmarks exists against which they could be measured. Some manufacturers provide benchmarks for individual sensors but we found no benchmarks that take a holistic view of the overall screening procedures and no benchmarks that take operator variability into account. Just adding up resources and manpower used is not an effective way for assessing systems where human decision-making and operator compliance to rules play a vital role. Our aim is to develop a decision support tool (cargo-screening system simulator) that will map the right technology and manpower to the right commodity-threat combination in order to maximise detection rates. In this paper we present our ideas for developing such a system and highlight the research challenges we have identified. Then we introduce our first case study and report on the progress we have made so far.

  19. Variable Structure Control of Catastrophic Course in Airdropping Heavy Cargo

    Institute of Scientific and Technical Information of China (English)

    Zhang Huiyuan; Shi Zhongke

    2009-01-01

    The mathematical model of a transport aircraft would be subjected to a sudden change when heavy cargo is dropped off in airdropping, which exerts serious influences upon the safety of the aircraft. A variable structure controller is specially designed for handling the airdrop process. The nonlinear system is linearized by input-output feedback linearization using differential geometry theories. On this basis, an inner loop system for velocity and attitude tracking control is designed by using the exponentially approaching rule of the variable structure theory. The whole flight control system is integrated with the outer loop flight altitude control. Digital simulation evidences the applicability of the system to potentially catastrophic course in airdropping heavy cargo and provides robustness against system parameter perturbation.

  20. The Liner Shipping Fleet Repositioning Problem with Cargo Flows

    DEFF Research Database (Denmark)

    Tierney, Kevin; Jensen, Rune Møller

    2012-01-01

    We solve an important problem for the liner shipping industry called the Liner Shipping Fleet Repositioning Problem (LSFRP). The LSFRP poses a large financial burden on liner shipping firms. During repositioning, vessels are moved between services in a liner shipping network. Shippers wish...... in the literature. We introduce a novel mathematical model of the LSFRP with cargo flows based on a carefully constructed graph and evaluate it on real world data from our industrial collaborator....

  1. August Passenger and Cargo Numbers Set New Highs

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ Passenger and cargo numbers set new highs in August, the second consecutive month that both areas of operation at Dragonair posted record figures. The airline flew 444,498 passengers in August to record its third consecutive monthly record. The number was 7.7%higher than in July, with travel in both months driven by holiday traffic. August 15 saw a new daily mark set, with 17,220passengers carried on the day.

  2. The Application Model of Moving Objects in Cargo Delivery System

    Institute of Scientific and Technical Information of China (English)

    ZHANG Feng-li; ZHOU Ming-tian; XU Bo

    2004-01-01

    The development of spatio-temporal database systems is primarily motivated by applications which track and present mobile objects. In this paper, solutions for establishing the moving object database based on GPS/GIS environment are presented, and a data modeling of moving object is given by using Temporal logical to extent the query language, finally the application model in cargo delivery system is shown.

  3. Shared Value Potential of Transporting Cargo via Hyperloop

    OpenAIRE

    Max Werner; Klaus Eissing; Sebastian Langton

    2016-01-01

    This research estimates the shared value created by constructing a hypothetical Hyperloop to transport cargo along 300 km in Northern Germany. Following Porter-Kramer (2011), we identified and evaluated eight factors that create shared value: travel speed, operating costs, safety, noise pollution, air pollution, climate effect/carbon footprint, separation effect/ property efficiency, and maintenance. Using official data compiled by several German institutes and organizations, we conducted com...

  4. X-Ray Scan Detection for Cargo Integrity

    Energy Technology Data Exchange (ETDEWEB)

    Valencia, Juan D.; Miller, Steven D.

    2011-04-18

    ABSTRACT The increase of terrorism and its global impact has made the determination of the contents of cargo containers a necessity. Existing technology allows non-intrusive inspections to determine the contents of a container rapidly and accurately. However, some cargo shipments are exempt from such inspections. Hence, there is a need for a technology that enables rapid and accurate means of detecting whether such containers were non-intrusively inspected. Non-intrusive inspections are most commonly performed utilizing high powered X-ray equipment. The challenge is creating a device that can detect short duration X-ray scans while maintaining a portable, battery powered, low cost, and easy to use platform. The Pacific Northwest National Laboratory (PNNL) has developed a methodology and prototype device focused on this challenge. The prototype, developed by PNNL, is a battery powered electronic device that continuously measures its X-ray and Gamma exposure, calculates the dose equivalent rate, and makes a determination of whether the device has been exposed to the amount of radiation experienced during an X-ray inspection. Once an inspection is detected, the device will record a timestamp of the event and relay the information to authorized personnel via a visual alert, USB connection, and/or wireless communication. The results of this research demonstrate that PNNL’s prototype device can be effective at determining whether a container was scanned by X-ray equipment typically used for cargo container inspections. This paper focuses on laboratory measurements and test results acquired with the PNNL prototype device using several X-ray radiation levels. Keywords: Radiation, Scan, X-ray, Gamma, Detection, Cargo, Container, Wireless, RF

  5. Compressed Sensing Techniques Applied to Ultrasonic Imaging of Cargo Containers.

    Science.gov (United States)

    López, Yuri Álvarez; Lorenzo, José Ángel Martínez

    2017-01-15

    One of the key issues in the fight against the smuggling of goods has been the development of scanners for cargo inspection. X-ray-based radiographic system scanners are the most developed sensing modality. However, they are costly and use bulky sources that emit hazardous, ionizing radiation. Aiming to improve the probability of threat detection, an ultrasonic-based technique, capable of detecting the footprint of metallic containers or compartments concealed within the metallic structure of the inspected cargo, has been proposed. The system consists of an array of acoustic transceivers that is attached to the metallic structure-under-inspection, creating a guided acoustic Lamb wave. Reflections due to discontinuities are detected in the images, provided by an imaging algorithm. Taking into consideration that the majority of those images are sparse, this contribution analyzes the application of Compressed Sensing (CS) techniques in order to reduce the amount of measurements needed, thus achieving faster scanning, without compromising the detection capabilities of the system. A parametric study of the image quality, as a function of the samples needed in spatial and frequency domains, is presented, as well as the dependence on the sampling pattern. For this purpose, realistic cargo inspection scenarios have been simulated.

  6. Compressed Sensing Techniques Applied to Ultrasonic Imaging of Cargo Containers

    Directory of Open Access Journals (Sweden)

    Yuri Álvarez López

    2017-01-01

    Full Text Available One of the key issues in the fight against the smuggling of goods has been the development of scanners for cargo inspection. X-ray-based radiographic system scanners are the most developed sensing modality. However, they are costly and use bulky sources that emit hazardous, ionizing radiation. Aiming to improve the probability of threat detection, an ultrasonic-based technique, capable of detecting the footprint of metallic containers or compartments concealed within the metallic structure of the inspected cargo, has been proposed. The system consists of an array of acoustic transceivers that is attached to the metallic structure-under-inspection, creating a guided acoustic Lamb wave. Reflections due to discontinuities are detected in the images, provided by an imaging algorithm. Taking into consideration that the majority of those images are sparse, this contribution analyzes the application of Compressed Sensing (CS techniques in order to reduce the amount of measurements needed, thus achieving faster scanning, without compromising the detection capabilities of the system. A parametric study of the image quality, as a function of the samples needed in spatial and frequency domains, is presented, as well as the dependence on the sampling pattern. For this purpose, realistic cargo inspection scenarios have been simulated.

  7. Compressed Sensing Techniques Applied to Ultrasonic Imaging of Cargo Containers

    Science.gov (United States)

    Álvarez López, Yuri; Martínez Lorenzo, José Ángel

    2017-01-01

    One of the key issues in the fight against the smuggling of goods has been the development of scanners for cargo inspection. X-ray-based radiographic system scanners are the most developed sensing modality. However, they are costly and use bulky sources that emit hazardous, ionizing radiation. Aiming to improve the probability of threat detection, an ultrasonic-based technique, capable of detecting the footprint of metallic containers or compartments concealed within the metallic structure of the inspected cargo, has been proposed. The system consists of an array of acoustic transceivers that is attached to the metallic structure-under-inspection, creating a guided acoustic Lamb wave. Reflections due to discontinuities are detected in the images, provided by an imaging algorithm. Taking into consideration that the majority of those images are sparse, this contribution analyzes the application of Compressed Sensing (CS) techniques in order to reduce the amount of measurements needed, thus achieving faster scanning, without compromising the detection capabilities of the system. A parametric study of the image quality, as a function of the samples needed in spatial and frequency domains, is presented, as well as the dependence on the sampling pattern. For this purpose, realistic cargo inspection scenarios have been simulated. PMID:28098841

  8. Diffusion-Limited Cargo Loading of an Engineered Protein Container.

    Science.gov (United States)

    Zschoche, Reinhard; Hilvert, Donald

    2015-12-30

    The engineered bacterial nanocompartment AaLS-13 is a promising artificial encapsulation system that exploits electrostatic interactions for cargo loading. In order to study its ability to take up and retain guests, a pair of fluorescent proteins was developed which allows spectroscopic determination of the extent of encapsulation by Förster resonance energy transfer (FRET). The encapsulation process is generally complete within a second, suggesting low energetic barriers for proteins to cross the capsid shell. Formation of intermediate aggregates upon mixing host and guest in vitro complicates capsid loading at low ionic strength, but can be sidestepped by increasing salt concentrations or diluting the components. Encapsulation of guests is completely reversible, and the position of the equilibrium is easily tuned by varying the ionic strength. These results, which challenge the notion that AaLS-13 is a continuous rigid shell, provide valuable information about cargo loading that will guide ongoing efforts to engineer functional host-guest complexes. Moreover, it should be possible to adapt the protein FRET pair described in this report to characterize functional capsid-cargo complexes generated by other encapsulation systems.

  9. pH and redox-operated nanovalve for size-selective cargo delivery on hollow mesoporous silica spheres.

    Science.gov (United States)

    Zhu, Xinyun; Wang, Cai-Qi

    2016-10-15

    A pH and redox dual-responsive nanovalve with a long stalk was introduced on the surface of hollow mesoporous silica nanoparticles (HMSs-S1) to achieve cargo size selectivity delivery. The responsive nanovalve was designed by constructing of a stalk/β-cyclodextrins (CDs) supramolecular complex, which is based on an acid-labile acetal group and the host-guest interactions between β-cyclodextrins and ferrocenyl moiety (Fc). With stimulation by different pH and H2O2, Rhodamine 6G showed well-responsive behavior. On account of the long stalks of nanovalve, doxorubicin hydrochloride and 5-fluorouracil with different sized cargos are encapsulated in HMSs-S1 to test its behavior of cargo size-selective delivery. Moreover the HMSs-S2 with a short stalk based on β-CDs/Fc inclusion complex is synthesized to load small sized 5-FU drug as contrast experiment. Compared with HMSs-S2, HMSs-S1 is compatible with larger drug molecules such as Rhodamine 6G (R6G) and doxorubicin hydrochloride (DOX), while small sized 5-fluorouracil (5-FU) is unable to be sealed by the nanovalve. Dual responsiveness and drug size selectivity make mechanized HMSs possess potential applications in drug delivery system.

  10. How molecular motors are arranged on a cargo is important for vesicular transport.

    Science.gov (United States)

    Erickson, Robert P; Jia, Zhiyuan; Gross, Steven P; Yu, Clare C

    2011-05-01

    The spatial organization of the cell depends upon intracellular trafficking of cargos hauled along microtubules and actin filaments by the molecular motor proteins kinesin, dynein, and myosin. Although much is known about how single motors function, there is significant evidence that cargos in vivo are carried by multiple motors. While some aspects of multiple motor function have received attention, how the cargo itself--and motor organization on the cargo--affects transport has not been considered. To address this, we have developed a three-dimensional Monte Carlo simulation of motors transporting a spherical cargo, subject to thermal fluctuations that produce both rotational and translational diffusion. We found that these fluctuations could exert a load on the motor(s), significantly decreasing the mean travel distance and velocity of large cargos, especially at large viscosities. In addition, the presence of the cargo could dramatically help the motor to bind productively to the microtubule: the relatively slow translational and rotational diffusion of moderately sized cargos gave the motors ample opportunity to bind to a microtubule before the motor/cargo ensemble diffuses out of range of that microtubule. For rapidly diffusing cargos, the probability of their binding to a microtubule was high if there were nearby microtubules that they could easily reach by translational diffusion. Our simulations found that one reason why motors may be approximately 100 nm long is to improve their 'on' rates when attached to comparably sized cargos. Finally, our results suggested that to efficiently regulate the number of active motors, motors should be clustered together rather than spread randomly over the surface of the cargo. While our simulation uses the specific parameters for kinesin, these effects result from generic properties of the motors, cargos, and filaments, so they should apply to other motors as well.

  11. How molecular motors are arranged on a cargo is important for vesicular transport.

    Directory of Open Access Journals (Sweden)

    Robert P Erickson

    2011-05-01

    Full Text Available The spatial organization of the cell depends upon intracellular trafficking of cargos hauled along microtubules and actin filaments by the molecular motor proteins kinesin, dynein, and myosin. Although much is known about how single motors function, there is significant evidence that cargos in vivo are carried by multiple motors. While some aspects of multiple motor function have received attention, how the cargo itself--and motor organization on the cargo--affects transport has not been considered. To address this, we have developed a three-dimensional Monte Carlo simulation of motors transporting a spherical cargo, subject to thermal fluctuations that produce both rotational and translational diffusion. We found that these fluctuations could exert a load on the motor(s, significantly decreasing the mean travel distance and velocity of large cargos, especially at large viscosities. In addition, the presence of the cargo could dramatically help the motor to bind productively to the microtubule: the relatively slow translational and rotational diffusion of moderately sized cargos gave the motors ample opportunity to bind to a microtubule before the motor/cargo ensemble diffuses out of range of that microtubule. For rapidly diffusing cargos, the probability of their binding to a microtubule was high if there were nearby microtubules that they could easily reach by translational diffusion. Our simulations found that one reason why motors may be approximately 100 nm long is to improve their 'on' rates when attached to comparably sized cargos. Finally, our results suggested that to efficiently regulate the number of active motors, motors should be clustered together rather than spread randomly over the surface of the cargo. While our simulation uses the specific parameters for kinesin, these effects result from generic properties of the motors, cargos, and filaments, so they should apply to other motors as well.

  12. Development of sup 6 sup 0 Co cargo train inspection system

    CERN Document Server

    Wu Zhi Fang

    2002-01-01

    The author introduces the research and development of sup 6 sup 0 Co cargo train inspection system. With the use of radiography principle, every car image is acquired when the cargo train runs through the inspection channel. It is evaluated whether the cargo in car matches the corresponding customs declaration information with digital image processing techniques. The system has been installed in railway port at Manzhouli Customs

  13. 49 CFR 180.413 - Repair, modification, stretching, rebarrelling, or mounting of specification cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... carbon dioxide, service are excepted from these testing requirements, but must be tested in accordance... rebuilt cargo tank's structural integrity, venting, and accident damage protection with the...

  14. 75 FR 64585 - Bulk Solid Hazardous Materials: Harmonization With the International Maritime Solid Bulk Cargoes...

    Science.gov (United States)

    2010-10-19

    ... nonsubstantive changes, however, to correct grammar, internal paragraph references, and a temperature conversion... means the English version of the ``International Maritime Solid Bulk Cargoes Code'' published by...

  15. ATHLETE: Lunar Cargo Handling for International Lunar Exploration

    Science.gov (United States)

    Wilcox, Brian H.

    2010-01-01

    As part of the Human-Robot Systems Project within the NASA Exploration Technology Development Program, the Jet Propulsion Laboratory is developing a vehicle called ATHLETE: the All-Terrain Hex-Limbed Extra-Terrestrial Explorer. The basic idea of ATHLETE is to have six relatively small wheels on the ends of legs. The small wheels and associated drive actuators are much less massive than the larger wheels and gears needed for an "all terrain" vehicle that cannot "walk" out of extreme terrain. The mass savings for the wheels and wheel actuators is greater than the mass penalty of the legs, for a net mass savings. Starting in 2009, NASA became engaged in detailed architectural studies for international discussions with the European Space Agency (ESA), the Japanese Space Agency (JAXA), and the Canadian Space Agency (CSA) under the auspices of the International Architecture Working Group (IAWG). ATHLETE is considered in most of the campaign options considered, providing a way to offload cargo from large Altair-class landers (having a cargo deck 6+ meters above the surface) as well as offloading international landers launched on Ariane-5 or H-2 launch vehicles. These international landers would carry provisions as well as scientific instruments and/or small rovers that would be used by international astronauts as part of an international effort to explore the moon.Work described in this paper includes architectural studies in support of the international missions as well as field testing of a half-scale ATHLETE prototype performing cargo offloading from a lander mockup, along with multi-kilometer traverse, climbing over greater than 1 m rocks, tool use, etc.

  16. Method of off-loading supporting for large aperture light weighted reflect mirror at optical axis horizontal situation%大口径轻质反射镜光轴水平卸载支撑方法

    Institute of Scientific and Technical Information of China (English)

    周于鸣; 赵野; 王海超; 闻广泉

    2013-01-01

      大口径轻质反射镜的重力环境下高精度检测是目前的一个难点,由于其轻量化程度高,绝对刚度低,在重力环境下,支撑形式的不同对光学检测结果影响巨大。提出了一种以提供提拉牵引力的方法实现光轴水平支撑测试的解决方案,通过一系列的滑轮组的自适应调整,获得预定义的力学支撑,实现轻质反射镜光轴水平状态重力环境下的高精度面形测试。有限元力学分析结果表明,依据本方法开展的光轴水平状态检测,能够有效减少重力引起的光轴竖直和光轴水平两种状态下的面形变化RMS值差异,其差异值小于0.003(=632.8 nm)。文中所提出的方法具有良好的可实施性,它可推广应用到其他大口径轻质反射镜的检测支撑的设计中,为此类大口径相机的研制提供技术基础。%  The precise testing of large aperture light weighted reflecting mirror in gravity environment is a difficult problem at present. In view of the high rate of light weighting and the absolute low stiffness, in this situation, different forms of support have huge impacts on optical test results in gravity environment. In this paper, a solution was suggested that pre-defining of supporting force was achieved through a series of pulley blocks self-adapting to solve large aperture mirror testing in gravity environment. The result of mechanics model analysis shows that the testing of optical axis horizontal can reduce the Rams variation of surface shape effectively, which is caused by gravity in vertical and horizontal state, and the variation less than 0.003 ( =632.8 nm). The method can be extended and applied to the design of testing supporting for other large aperture light weighted reflect mirror, and provid; technology foundation for large aperture mirror.

  17. Solving the Liner Shipping Fleet Repositioning Problem with Cargo Flows

    DEFF Research Database (Denmark)

    Tierney, Kevin; Askelsdottir, Björg; Jensen, Rune Møller

    2015-01-01

    We solve a central problem in the liner shipping industry called the liner shipping fleet repositioning problem (LSFRP). The LSFRP poses a large financial burden on liner shipping firms. During repositioning, vessels are moved between routes in a liner shipping network. Liner carriers wish...... introduce a novel mathematical model and a simulated annealing algorithm for the LSFRP with cargo flows that makes use of a carefully constructed graph; we evaluate these approaches using real-world data from our industrial collaborator. Additionally, we compare the performance of our approach against...

  18. Domestic & International Air Cargo Activity: National and Selected Hub Forecasts.

    Science.gov (United States)

    1979-11-01

    world regional models. These six world regions are: North America excluding U.S., South America, Europe, Asia, Australia and Oceania, and Africa . For each...1 I et p(B) p(B) = (I- PIB - P2B 2,---, PpB ) and B is a backward shift operator. The part f (Xt’s 1 ) is a predictable component, which contains the...world regions are: North America excluding U.S., South America, Europe, Asia, Australia and Oceania and Africa . Statistics of air cargo flow for these

  19. Collinear swimmer propelling a cargo sphere at low Reynolds number

    CERN Document Server

    Felderhof, B U

    2014-01-01

    The swimming velocity and rate of dissipation of a linear chain consisting of two or three little spheres and a big sphere is studied on the basis of low Reynolds number hydrodynamics. The big sphere is treated as a passive cargo, driven by the tail of little spheres via hydrodynamic and direct elastic interaction. The fundamental solution of Stokes' equations in the presence of a sphere with no-slip boundary condition, as derived by Oseen, is used to model the hydrodynamic interactions between the big sphere and the little spheres.

  20. Optimization of the imported air express cargo distribution problem

    Directory of Open Access Journals (Sweden)

    Hwang, T.L.

    2013-03-01

    Full Text Available This study examines the delivering network of imported air express cargo as an integrated multi-depot vehicle routing problem. Integrated multi-depot vehicle routing problem attempts to decide which service centers should be used and how much freight should be unloaded in each service center. The role of an exchange point which is allowing the delivery vans and shuttles to exchange imported and exported goods is also addressed. Test results demonstrate the feasibility of the four models so these are highly promising for use in a diverse array of applications, such as in home delivery and reverse logistics.

  1. Achieving integrated convoys: cargo unmanned ground vehicle development and experimentation

    Science.gov (United States)

    Zych, Noah; Silver, David; Stager, David; Green, Colin; Pilarski, Thomas; Fischer, Jacob

    2013-05-01

    The Cargo UGV project was initiated in 2010 with the aim of developing and experimenting with advanced autonomous vehicles capable of being integrated unobtrusively into manned logistics convoys. The intent was to validate two hypotheses in complex, operationally representative environments: first, that unmanned tactical wheeled vehicles provide a force protection advantage by creating standoff distance to warfighters during ambushes or improvised explosive device attacks; and second, that these UGVs serve as force multipliers by enabling a single operator to control multiple unmanned assets. To assess whether current state-of-the-art autonomous vehicle technology was sufficiently capable to permit resupply missions to be executed with decreased risk and reduced manpower, and to assess the effect of UGVs on customary convoy tactics, the Marine Corps Warfighting Laboratory and the Joint Ground Robotics Enterprise sponsored Oshkosh Defense and the National Robotics Engineering Center to equip two standard Marine Corps cargo trucks for autonomous operation. This paper details the system architecture, hardware implementation, and software modules developed to meet the vehicle control, perception, and planner requirements compelled by this application. Additionally, the design of a custom human machine interface and an accompanying training program are described, as is the creation of a realistic convoy simulation environment for rapid system development. Finally, results are conveyed from a warfighter experiment in which the effectiveness of the training program for novice operators was assessed, and the impact of the UGVs on convoy operations was observed in a variety of scenarios via direct comparison to a fully manned convoy.

  2. Functional architecture of the retromer cargo-recognition complex

    Science.gov (United States)

    Hierro, Aitor; Rojas, Adriana L.; Rojas, Raul; Murthy, Namita; Effantin, Grégory; Kajava, Andrey V.; Steven, Alasdair C.; Bonifacino, Juan S.; Hurley, James H.

    2008-01-01

    The retromer complex 1, 2 is required for the sorting of acid hydrolases to lysosomes 3-7, transcytosis of the polymeric Ig receptor 8, Wnt gradient formation 9, 10, iron transporter recycling 11, and processing of the amyloid precursor protein 12. Human retromer consists of two smaller complexes, the cargo recognition Vps26:Vps29:Vps35 heterotrimer, and a membrane-targeting heterodimer or homodimer of SNX1 and/or SNX2 13. The crystal structure of a Vps29:Vps35 subcomplex shows how the metallophosphoesterase-fold subunit Vps29 14, 15 acts as a scaffold for the C-terminal half of Vps35. Vps35 forms a horseshoe-shaped right-handed α-helical solenoid whose concave face completely covers the metal-binding site of Vps29 and whose convex face exposes a series of hydrophobic interhelical grooves. Electron microscopy shows that the intact Vps26:Vps29:Vps35 complex is a stick-shaped, somewhat flexible, structure, ∼ 21 nm long. A hybrid structural model derived from crystal structures, electron microscopy, interaction studies, and bioinformatics shows that the α-solenoid fold extends the full length of Vps35, and that Vps26 is bound at the opposite end from Vps29. This extended structure presents multiple binding sites for the SNX complex and receptor cargo, and appears capable of flexing to conform to curved vesicular membranes. PMID:17891154

  3. Direct strength assessment of foremost and aftmost cargo%最首尾货舱直接强度评估研究

    Institute of Scientific and Technical Information of China (English)

    顾俊; 吴嘉蒙; 陈倩; 张帆; 蔡诗剑

    2015-01-01

    This paper studies and analyzes the technical issues about the ifnite element analysis of the foremost and aftmost cargo according to Harmonised Common Structural Rules (CSR-H). The theoretical analysis combined with the numerical experiments are carried out to assess the rationality of the boundary conditions, mechanical models and model extension in the ifnite element evaluation of the foremost and aftmost cargo. The strength of the foremost and aftmost cargo is directly evaluated through the numerical experiments of current constructed vessels. The numerical results show that warp effect can be closely simulated with simply ifxed boundary conditions at both ends, and the model extension has an inlfuence on the strength assessment results. The foremost cargo that is close to the boundary will result in the unacceptable buckling strength of deck and shell plate, which is one of the reasons causing the assessment failure of buckling strength of the foremost cargo. The shear force adjustment for the aftmost cargo by CSR-H is still based on the middle cargo, which is not applicable because only the shear stresses at fore and aft bulkhead are taken as the target values for the adjustment.%针对CSR-H关于最首尾货舱有限元分析在实际评估中可能存在的技术问题进行研究分析。通过理论分析与数值试验相结合对最首尾货舱有限元评估方法中的边界条件、力学模型、模型范围进行合理性评估,通过已建造船数值试验对最首尾货舱开展有限元直接强度评估。数值分析结果表明两端简支的边界条件能较好的模拟翘曲效应,模型范围对强度评估结果有影响。最首货舱离边界很近会导致甲板、外板的屈曲强度不满足要求。而CSR-H针对最尾货舱有限元中的剪力调整方法仍基于货舱中段,仅以货舱前后端壁剪力为目标值进行调整,这种剪力调整方法不适用于最尾货舱。

  4. Strong Interest In China’s Cargo Market——An interview with Jean-Peter Jansen, chairman of the Executive Board of Lufthansa Cargo AG.

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    <正>Lufthansa Cargo AG ranks top among European airlines in the flights bound for China. It has 14 weekly flights for Hong Kong to transport 720 tons of cargo and mails, nine weekly services for Shanghai to carry 450 tons and four weekly flights for Beijing to handle 250 tons.The carrier is showing strong interest in the Chinese market, for 11 per cent of its sales in 2003, 2.16 billion euro came from China.

  5. 33 CFR 126.29 - Supervision and control of dangerous cargo.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Supervision and control of... Supervision and control of dangerous cargo. (a) Authority. The Captain of the Port is authorized to require... cargo covered by this subchapter shall be undertaken and continued only under the immediate...

  6. Co-operative versus independent transport of different cargoes by Kinesin-1.

    Science.gov (United States)

    Hammond, Jennetta W; Griffin, Kelly; Jih, Gloria T; Stuckey, Jeanne; Verhey, Kristen J

    2008-05-01

    Kinesin motors drive the intracellular transport of multiple cargoes along microtubule tracks; yet, how kinesins discriminate among their many potential cargoes is unknown. We tested whether Kinesin-1 cargoes compete, co-operate or are transported independently of each other. We focused on Kinesin-1 cargoes that bind directly to the kinesin light chain (KLC) subunit, namely the c-Jun NH(2)-terminal kinase-interacting proteins (JIPs) 1 and 3, Kidins220/ARMS and PAT1. Overexpression of individual cargo proteins in differentiated CAD cells resulted in mislocalization of the endogenous protein but had no effect on localization of other cargo proteins to neurite tips. Thus, while transport of distinct cargoes is saturable, they do not compete with each other. Interestingly, we found that low expression of JIP1 or JIP3 enhanced the transport of the other JIP to neurite tips. Moreover, JIP1 and JIP3 require each other for transport. Co-operative transport is due to an interaction between JIP1 and JIP3 as well as distinct binding sites on the KLC tetratricopeptide repeat (TPR) bundle: the TPR groove binds to C-terminal residues of JIP1, whereas the TPR surface binds to internal residues in JIP3. Formation of a JIP1/JIP3/KLC complex is necessary for efficient JIP1 or JIP3 transport in neuronal cells. Thus, JIP scaffolding proteins are transported in a co-operative manner, despite the independent transport of other Kinesin-1 cargoes.

  7. 75 FR 59617 - Notification of Arrival in U.S. Ports; Certain Dangerous Cargoes

    Science.gov (United States)

    2010-09-28

    ... requirements. The 2005 definition of CDC residue only included residue quantities of bulk ammonium nitrate or ammonium nitrate fertilizer that remained onboard after the vessel discharges all saleable cargo; no other... until the removal of all bulk liquid and liquefied gas CDC cargoes, including residue quantities of...

  8. 46 CFR 154.1710 - Exclusion of air from cargo tank vapor spaces.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Exclusion of air from cargo tank vapor spaces. 154.1710... Operating Requirements § 154.1710 Exclusion of air from cargo tank vapor spaces. When a vessel is carrying acetaldehyde, butadiene, ethylene oxide, or vinyl chloride, the master shall ensure that air is: (a)...

  9. 46 CFR 308.551 - War risk insurance clearing agency agreement for cargo, Form MA-321.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false War risk insurance clearing agency agreement for cargo... EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Iv-General § 308.551 War risk insurance... American War Risk Agency or MARAD....

  10. 77 FR 35993 - Agency Information Collection Activities: Cargo Container and Road Vehicle Certification for...

    Science.gov (United States)

    2012-06-15

    ... affected agencies. This information collection was previously published in the Federal Register (77 FR... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Cargo Container and... Budget (OMB) for review and approval in accordance with the Paperwork Reduction Act: Cargo Container...

  11. 46 CFR 153.981 - Leaving room in tank for cargo expansion.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Leaving room in tank for cargo expansion. 153.981 Section 153.981 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS... Transfer Procedures § 153.981 Leaving room in tank for cargo expansion. The person in charge of...

  12. 46 CFR 153.602 - Special requirements for cargoes reactive with water.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Special requirements for cargoes reactive with water. 153.602 Section 153.602 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK... and Equipment Special Requirements § 153.602 Special requirements for cargoes reactive with...

  13. 77 FR 19030 - Automated Commercial Environment Required for the Transmission of Advance Ocean and Rail Cargo...

    Science.gov (United States)

    2012-03-29

    .... 12-06] Automated Commercial Environment Required for the Transmission of Advance Ocean and Rail Cargo... Automated Commercial Environment (ACE) for the transmission of advance ocean and rail cargo information... controlled environment.\\3\\ M1 test participants were chosen based on the specific type of software...

  14. 78 FR 50134 - Altus Pharmaceuticals, Inc., Blackhawk Capital Group BDC, Inc., Cargo Connection Logistics...

    Science.gov (United States)

    2013-08-16

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Altus Pharmaceuticals, Inc., Blackhawk Capital Group BDC, Inc., Cargo Connection Logistics Holding... securities of Cargo Connection Logistics Holding, Inc. because it has not filed any periodic reports...

  15. CargoVibes: human response to vibration due to freight rail traffic

    NARCIS (Netherlands)

    Waddington, D.; Woodcock, J.; Smith, M.G.; Janssen, S.A.; Persson Waye, K.

    2015-01-01

    The aim of this paper is to present an overview of the research concerning human response to vibration conducted in the EU FP7 CargoVibes project. The European Union-funded project CargoVibes involved 10 partners from 8 nations and ran from April 2011 to April 2014. The project was concerned with ra

  16. Neutron interrogation system using high gamma ray signature to detect contraband special nuclear materials in cargo

    Science.gov (United States)

    Slaughter, Dennis R.; Pohl, Bertram A.; Dougan, Arden D.; Bernstein, Adam; Prussin, Stanley G.; Norman, Eric B.

    2008-04-15

    A system for inspecting cargo for the presence of special nuclear material. The cargo is irradiated with neutrons. The neutrons produce fission products in the special nuclear material which generate gamma rays. The gamma rays are detecting indicating the presence of the special nuclear material.

  17. Extensive cargo identification reveals distinct biological roles of the 12 importin pathways

    Science.gov (United States)

    Kimura, Makoto; Morinaka, Yuriko; Imai, Kenichiro; Kose, Shingo; Horton, Paul; Imamoto, Naoko

    2017-01-01

    Vast numbers of proteins are transported into and out of the nuclei by approximately 20 species of importin-β family nucleocytoplasmic transport receptors. However, the significance of the multiple parallel transport pathways that the receptors constitute is poorly understood because only limited numbers of cargo proteins have been reported. Here, we identified cargo proteins specific to the 12 species of human import receptors with a high-throughput method that employs stable isotope labeling with amino acids in cell culture, an in vitro reconstituted transport system, and quantitative mass spectrometry. The identified cargoes illuminated the manner of cargo allocation to the receptors. The redundancies of the receptors vary widely depending on the cargo protein. Cargoes of the same receptor are functionally related to one another, and the predominant protein groups in the cargo cohorts differ among the receptors. Thus, the receptors are linked to distinct biological processes by the nature of their cargoes. DOI: http://dx.doi.org/10.7554/eLife.21184.001 PMID:28117667

  18. 76 FR 4709 - Extension of Agency Information Collection Activity Under OMB Review: Certified Cargo Screening...

    Science.gov (United States)

    2011-01-26

    ... off- airport and includes facilities upstream in the air cargo supply chain, such as shippers, manufacturers, warehousing entities, distributors, third party logistics companies, and Indirect Air Carriers... supply chain to accept, screen, and transport air cargo. The uninterrupted collection of this...

  19. 46 CFR 308.523 - Application for revision of Open Cargo Policy, Form MA-303.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Application for revision of Open Cargo Policy, Form MA-303. 308.523 Section 308.523 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY... Application for revision of Open Cargo Policy, Form MA-303. An application for the revision of an Open...

  20. 46 CFR 308.548 - Standard form of underwriting agency agreement for cargo, Form MA-318.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Standard form of underwriting agency agreement for cargo, Form MA-318. 308.548 Section 308.548 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... underwriting agency agreement for cargo, Form MA-318. This form, which may be obtained from the American...

  1. 46 CFR 308.545 - Facultative cargo policy, Form MA-316.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Facultative cargo policy, Form MA-316. 308.545 Section 308.545 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK... policy, Form MA-316. The standard form of War Risk Facultative Cargo Policy, Form MA-316, may be...

  2. 46 CFR 308.524 - Application for cancellation of Open Cargo Policy, Form MA-304.

    Science.gov (United States)

    2010-10-01

    ... MA-304. 308.524 Section 308.524 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... § 308.524 Application for cancellation of Open Cargo Policy, Form MA-304. The standard form of application for cancellation of an Open Cargo Policy Form MA-304 may be obtained from the American War...

  3. 46 CFR 38.10-10 - Cargo piping-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo piping-TB/ALL. 38.10-10 Section 38.10-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS LIQUEFIED FLAMMABLE GASES Piping, Valves, Fittings, and Accessory Equipment § 38.10-10 Cargo piping—TB/ALL. (a) The piping shall be designed for...

  4. 76 FR 14643 - Hazardous Materials: Safety Requirements for External Product Piping on Cargo Tanks Transporting...

    Science.gov (United States)

    2011-03-17

    ...: Safety Requirements for External Product Piping on Cargo Tanks Transporting Flammable Liquids AGENCY... external product piping (wetlines) on a cargo tank motor vehicle (CTMV) unless the CTMV is equipped with... National Tank Truck Carriers, Inc., and the Tank Truck Manufacturers Association requesting an extension...

  5. 77 FR 21579 - Agency Information Collection Activities: Transfer of Cargo to a Container Station

    Science.gov (United States)

    2012-04-10

    ... a Container Station AGENCY: U.S. Customs and Border Protection, Department of Homeland Security... and approval in accordance with the Paperwork Reduction Act: Transfer of Cargo to a Container Station... forms of information. Title: Transfer of Cargo to a Container Station. OMB Number: 1651-0096....

  6. Mechanics

    CERN Document Server

    Hartog, J P Den

    1961-01-01

    First published over 40 years ago, this work has achieved the status of a classic among introductory texts on mechanics. Den Hartog is known for his lively, discursive and often witty presentations of all the fundamental material of both statics and dynamics (and considerable more advanced material) in new, original ways that provide students with insights into mechanical relationships that other books do not always succeed in conveying. On the other hand, the work is so replete with engineering applications and actual design problems that it is as valuable as a reference to the practicing e

  7. VAMP4 Is an Essential Cargo Molecule for Activity-Dependent Bulk Endocytosis.

    Science.gov (United States)

    Nicholson-Fish, Jessica C; Kokotos, Alexandros C; Gillingwater, Thomas H; Smillie, Karen J; Cousin, Michael A

    2015-12-02

    The accurate formation of synaptic vesicles (SVs) and incorporation of their protein cargo during endocytosis is critical for the maintenance of neurotransmission. During intense neuronal activity, a transient and acute accumulation of SV cargo occurs at the plasma membrane. Activity-dependent bulk endocytosis (ADBE) is the dominant SV endocytosis mode under these conditions; however, it is currently unknown how ADBE mediates cargo retrieval. We examined the retrieval of different SV cargo molecules during intense stimulation using a series of genetically encoded pH-sensitive reporters in neuronal cultures. The retrieval of only one reporter, VAMP4-pHluorin, was perturbed by inhibiting ADBE. This selective recovery was confirmed by the enrichment of endogenous VAMP4 in purified bulk endosomes formed by ADBE. VAMP4 was also essential for ADBE, with a cytoplasmic di-leucine motif being critical for this role. Therefore, VAMP4 is the first identified ADBE cargo and is essential for this endocytosis mode to proceed.

  8. A novel architecture for cargos location and safety in networked RFID

    Institute of Scientific and Technical Information of China (English)

    HUANG Dong-li; YUAN Zheng-wu; Byeong-Seob You; Sook-Kyung Cho; Hae-Young Bae

    2007-01-01

    Using Networked RFID equipped in warehouse or supermarket distribution, Enterprise information system (EIS) can gather and deal with cargos information. Nevertheless, when cargos are in traffic, the Networked RFID can't monitor the cargos any more as the products tagged aren't within range of a reader. This paper proposes a solution framework which combines Networked RFID and GPS tracking, and then equip with this system in container to make the cargos monitored even if the cargos are in traffic. This solution can solve the accurate consignments, position tracking and advanced theft happening through drilling holes in container. In addition, customhouse and checkpoint can get an electronic shipment manifest rapidly and safely when a vehicle gets through a checkpoint.

  9. Corrosion Behavior of Welded Joints for Cargo Oil Tanks of Crude Oil Carrier

    Institute of Scientific and Technical Information of China (English)

    Jin-shan WEI; Yan-chang QI; Zhi-ling TIAN; Yun PENG

    2016-01-01

    E32 grade corrosion resistant steel was welded with welding wires with three different S contents.The mi-crostructure,mechanical properties,inclusions,and corrosion behavior of welded joint were investigated.The joint coupon corrosion test and potentiodynamic polarization test were carried out under the simulated corrosion environ-ment of the inner bottom plates of cargo oil tanks.The pitting initiation and propagation mechanism of the weld metal were studied by scanning electron microscopy and infinite focus.The results indicated that the microstructures of three kinds of weld metals are all composed of acicular ferrite,ferrite side-plate and proeutectoid ferrite.The micro-structure of heat-affected zone is composed predominantly of bainite.Joint welded with low S filler wire has good me-chanical properties.S can decrease free corrosion potential and increase the corrosion tendency.The pitting initiation is oxide inclusion or sulfide-oxide inclusion complex.S can induce the formation of occluded area and promote the corrosion propagation.The chemical compositions of weld metal is similar to base metal,which can limit the galvanic corrosion between weld metal and base metal,and avoid formation of corrosion step.

  10. 46 CFR 38.05-3 - Design and construction of pressure vessel type cargo tanks-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Design and construction of pressure vessel type cargo... LIQUEFIED FLAMMABLE GASES Design and Installation § 38.05-3 Design and construction of pressure vessel type cargo tanks—TB/ALL. (a) Cargo tanks of pressure vessel configuration (e.g. cylindrical, spherical,...

  11. 49 CFR 175.702 - Separation distance requirements for packages containing Class 7 (radioactive) materials in cargo...

    Science.gov (United States)

    2010-10-01

    ... containing Class 7 (radioactive) materials in cargo aircraft. 175.702 Section 175.702 Transportation Other... (radioactive) materials in cargo aircraft. (a) No person may carry in a cargo aircraft any package required by... separation distance between the surfaces of the radioactive materials packages, overpacks or...

  12. 78 FR 21037 - Special Conditions: Airbus Model A330-200 Airplanes; Bulk Cargo Lower Deck Crew Rest Compartments

    Science.gov (United States)

    2013-04-09

    ... Cargo Lower Deck Crew Rest Compartments AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... unusual design feature associated with the installation of bulk cargo lower deck crew rest compartments... supplemental type certificate to install a bulk cargo lower deck crew rest compartment in the Airbus Model...

  13. 49 CFR 176.84 - Other requirements for stowage and segregation for cargo vessels and passenger vessels.

    Science.gov (United States)

    2010-10-01

    ... May be stowed in portable magazine or metal locker. 16 No other cargo may be stowed in the same hold... solids. 101 Stow “separated from” iron oxide. 102 Stow “separated from” all odor absorbing cargoes. 103... must be steel. 15E On deck, cargo transport unit must be leakproof. 17E On deck stowage is...

  14. PINS Measurements of Explosive Simulants for Cargo Screening

    Energy Technology Data Exchange (ETDEWEB)

    E.H. Seabury

    2008-06-01

    As part of its efforts to prevent the introduction of explosive threats on commercial flights, the Transportation Security Administration (TSL) is evaluating new explosives detection systems (EDSs) for use in air cargo inspection. The TSL has contracted Battelle to develop a new type of explosives simulant to assist in this development. These are designed to mimic the elemental profile (C, H, N, O, etc.) of explosives as well as their densities. Several “neutron in—gamma out” (n,?) techniques have been considered to quantify the elemental profile in these new simulants and the respective explosives. The method chosen by Battelle is Portable Isotopic Neutron Spectroscopy (PINS), developed by Idaho National Laboratory (INL). Battelle wishes to validate that the simulants behave like the explosive threats with this technology. The results of the validation measurements are presented in this report.

  15. ATHLETE: A Cargo-Handling Vehicle for Solar System Exploration

    Science.gov (United States)

    Wilcox, Brian H.

    2011-01-01

    As part of the NASA Exploration Technology Development Program, the Jet Propulsion Laboratory is developing a vehicle called ATHLETE: the All-Terrain Hex-Limbed Extra-Terrestrial Explorer. Each vehicle is based on six wheels at the ends of six multi-degree-of-freedom limbs. Because each limb has enough degrees of freedom for use as a general-purpose leg, the wheels can be locked and used as feet to walk out of excessively soft or other extreme terrain. Since the vehicle has this alternative mode of traversing through or at least out of extreme terrain, the wheels and wheel actuators can be sized for nominal terrain. There are substantial mass savings in the wheel and wheel actuators associated with designing for nominal instead of extreme terrain. These mass savings are comparable-to or larger-than the extra mass associated with the articulated limbs. As a result, the entire mobility system, including wheels and limbs, can be about 25% lighter than a conventional mobility chassis. A side benefit of this approach is that each limb has sufficient degrees-of-freedom to use as a general-purpose manipulator (hence the name "limb" instead of "leg"). Our prototype ATHLETE vehicles have quick-disconnect tool adapters on the limbs that allow tools to be drawn out of a "tool belt" and maneuvered by the limb. A power-take-off from the wheel actuates the tools, so that they can take advantage of the 1+ horsepower motor in each wheel to enable drilling, gripping or other power-tool functions. Architectural studies have indicated that one useful role for ATHLETE in planetary (moon or Mars) exploration is to "walk" cargo off the payload deck of a lander and transport it across the surface. Recent architectural approaches are focused on the concept that the lander descent stage will use liquid hydrogen as a propellant. This is the highest performance chemical fuel, but it requires very large tanks. A natural geometry for the lander is to have a single throttleable rocket engine on

  16. Advanced analysis of the transverse bulkhead of the a general cargo ship

    Science.gov (United States)

    Axinte, T.; Nutu, C.; Stanca, C.; Cupsa, O.; Carp, A.

    2016-08-01

    The paper presents the main strength factors stressing the transverse bulkhead of a cargo ship. The transverse bulkhead is one of the main components of the hull for a general cargo vessel. There are presented the role and the importance of the general cargo vessel's type using an original drawing made in NX 8.0 Software from Siemens. Further on, we are presented the importance of the transverse bulkhead of the general cargo ship's hull. Next there are analysed the formability of the transverse bulkhead's material and then we are determining the shear, normal and von Mises stresses in the traverse bulkhead, using the finite element method. Once the stresses are computed, there are also determined the fatigue life, strength safety factor and fatigue safety factor. The analysis of the transverse bulkhead is important for the safety of the general cargo vessel type because if this component of the ship does not resist to the various stresses and high deformations during bad storms on the sea or if the cargo is stored inappropriately, deformations or even breakage of the transverse bulkhead can occur, thus causing damages of the cargo ship, especially under heavy storm.

  17. Redox-regulated cargo binding and release by the peroxisomal targeting signal receptor, Pex5.

    Science.gov (United States)

    Ma, Changle; Hagstrom, Danielle; Polley, Soumi Guha; Subramani, Suresh

    2013-09-20

    In its role as a mobile receptor for peroxisomal matrix cargo containing a peroxisomal targeting signal called PTS1, the protein Pex5 shuttles between the cytosol and the peroxisome lumen. Pex5 binds PTS1 proteins in the cytosol via its C-terminal tetratricopeptide domains and delivers them to the peroxisome lumen, where the receptor·cargo complex dissociates. The cargo-free receptor is exported to the cytosol for another round of import. How cargo release and receptor recycling are regulated is poorly understood. We found that Pex5 functions as a dimer/oligomer and that its protein interactions with itself (homo-oligomeric) and with Pex8 (hetero-oligomeric) control the binding and release of cargo proteins. These interactions are controlled by a redox-sensitive amino acid, cysteine 10 of Pex5, which is essential for the formation of disulfide bond-linked Pex5 forms, for high affinity cargo binding, and for receptor recycling. Disulfide bond-linked Pex5 showed the highest affinity for PTS1 cargo. Upon reduction of the disulfide bond by dithiothreitol, Pex5 transitioned to a noncovalent dimer, concomitant with the partial release of PTS1 cargo. Additionally, dissipation of the redox balance between the cytosol and the peroxisome lumen caused an import defect. A hetero-oligomeric interaction between the N-terminal domain (amino acids 1-110) of Pex5 and a conserved motif at the C terminus of Pex8 further facilitates cargo release, but only under reducing conditions. This interaction is also important for the release of PTS1 proteins. We suggest a redox-regulated model for Pex5 function during the peroxisomal matrix protein import cycle.

  18. EL VÍNCULO INDIVIDUO-COLECTIVO EN EL SISTEMA DE CARGOS EN UNA COMUNIDAD RURAL DEL SUR DE TLAXCALA, MÉXICO

    Directory of Open Access Journals (Sweden)

    Hernán Salas Quintanal

    2013-12-01

    Full Text Available This paper exposes the authors reflections about their last investigations into  community organization concept. The research took place in several countries in Mexico from 2008 to 2012. It is an anthropological analysis about cargo system, which includes native and no-native communities. In such a way, the authors recognize the importance of heterogeneous institution into cargo system concept. So that, they suggest, as the main hypothesis, that cargo system creates several mechanisms, which promote that members of the community stimulated social interactions’ scenes into role set. First, the community institutions promote the participation of the largest number of people. Second, interactions’ scenes are constantly created and recreated by the community members. Third, the roles’ rotation, as a product of a tacit agreement into community members, is fundamental to assure social participation, scene by scene. In order to facilitate the analysis, such mechanisms have been categorised in four larger dimensions: political-judicial, economic, religious and social. As a final thought, the idea of a communitarian project becomes the common characteristic of all the studied communities.

  19. Myosin VI regulates actin structure specialization through conserved cargo-binding domain sites.

    Directory of Open Access Journals (Sweden)

    Mamiko Isaji

    Full Text Available Actin structures are often stable, remaining unchanged in organization for the lifetime of a differentiated cell. Little is known about stable actin structure formation, organization, or maintenance. During Drosophila spermatid individualization, long-lived actin cones mediate cellular remodeling. Myosin VI is necessary for building the dense meshwork at the cones' fronts. We test several ideas for myosin VI's mechanism of action using domain deletions or site-specific mutations of myosin VI. The head (motor and globular tail (cargo-binding domains were both needed for localization at the cone front and dense meshwork formation. Several conserved partner-binding sites in the globular tail previously identified in vertebrate myosin VI were critical for function in cones. Localization and promotion of proper actin organization were separable properties of myosin VI. A vertebrate myosin VI was able to localize and function, indicating that functional properties are conserved. Our data eliminate several models for myosin VI's mechanism of action and suggest its role is controlling organization and action of actin assembly regulators through interactions at conserved sites. The Drosophila orthologues of interaction partners previously identified for vertebrate myosin VI are likely not required, indicating novel partners mediate this effect. These data demonstrate that generating an organized and functional actin structure in this cell requires multiple activities coordinated by myosin VI.

  20. Tackling the x-ray cargo inspection challenge using machine learning

    Science.gov (United States)

    Jaccard, Nicolas; Rogers, Thomas W.; Morton, Edward J.; Griffin, Lewis D.

    2016-05-01

    The current infrastructure for non-intrusive inspection of cargo containers cannot accommodate exploding com-merce volumes and increasingly stringent regulations. There is a pressing need to develop methods to automate parts of the inspection workflow, enabling expert operators to focus on a manageable number of high-risk images. To tackle this challenge, we developed a modular framework for automated X-ray cargo image inspection. Employing state-of-the-art machine learning approaches, including deep learning, we demonstrate high performance for empty container verification and specific threat detection. This work constitutes a significant step towards the partial automation of X-ray cargo image inspection.

  1. Mechanics

    CERN Document Server

    Chester, W

    1979-01-01

    When I began to write this book, I originally had in mind the needs of university students in their first year. May aim was to keep the mathematics simple. No advanced techniques are used and there are no complicated applications. The emphasis is on an understanding of the basic ideas and problems which require expertise but do not contribute to this understanding are not discussed. How­ ever, the presentation is more sophisticated than might be considered appropri­ ate for someone with no previous knowledge of the subject so that, although it is developed from the beginning, some previous acquaintance with the elements of the subject would be an advantage. In addition, some familiarity with element­ ary calculus is assumed but not with the elementary theory of differential equations, although knowledge of the latter would again be an advantage. It is my opinion that mechanics is best introduced through the motion of a particle, with rigid body problems left until the subject is more fully developed. Howev...

  2. Lemur tyrosine kinase-2 signalling regulates kinesin-1 light chain-2 phosphorylation and binding of Smad2 cargo.

    LENUS (Irish Health Repository)

    Manser, C

    2012-05-31

    A recent genome-wide association study identified the gene encoding lemur tyrosine kinase-2 (LMTK2) as a susceptibility gene for prostate cancer. The identified genetic alteration is within intron 9, but the mechanisms by which LMTK2 may impact upon prostate cancer are not clear because the functions of LMTK2 are poorly understood. Here, we show that LMTK2 regulates a known pathway that controls phosphorylation of kinesin-1 light chain-2 (KLC2) by glycogen synthase kinase-3β (GSK3β). KLC2 phosphorylation by GSK3β induces the release of cargo from KLC2. LMTK2 signals via protein phosphatase-1C (PP1C) to increase inhibitory phosphorylation of GSK3β on serine-9 that reduces KLC2 phosphorylation and promotes binding of the known KLC2 cargo Smad2. Smad2 signals to the nucleus in response to transforming growth factor-β (TGFβ) receptor stimulation and transport of Smad2 by kinesin-1 is required for this signalling. We show that small interfering RNA loss of LMTK2 not only reduces binding of Smad2 to KLC2, but also inhibits TGFβ-induced Smad2 signalling. Thus, LMTK2 may regulate the activity of kinesin-1 motor function and Smad2 signalling.

  3. 49 CFR 180.407 - Requirements for test and inspection of specification cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... appurtenances and structural attachments on the cargo tank including, but not limited to, suspension system...; (vii) Areas near upper coupler (fifth wheel) assembly attachments; (viii) Areas near suspension system... PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS...

  4. Opportunity of Constructing a Cargo Terminal – Case Study Brașov International Airport, Romania

    Directory of Open Access Journals (Sweden)

    Gabriel BRĂTUCU

    2017-02-01

    Full Text Available Air transport and especially air cargo transport became ever more important during the last 25 years, with a signifi cant impact upon economic development. Air cargo transport supports regional economic development, provided there is suffi cient local demand for its services. In our study, we have identifi ed signifi cant elements of the impact a cargo terminal at the International Brașov-Ghimbav Airport would have upon local economic development. For this purpose, we have conducted a quantitative market research. We have reached the conclusion that constructing a cargo terminal at the Brașov-Ghimbav Airport is a necessary investment in the current economic context, with an important contribution to the economic development of its surrounding counties. The positive results registered in our study could contribute to improving the economic and fi nancial prospects of Brașov-Ghimbav Airport and attract investors in this project.

  5. Bidirectional transport of motor-driven cargoes in cell: A random walk with memory

    Science.gov (United States)

    Bhat, Deepak; Gopalakrishnan, Manoj

    2013-02-01

    Motor-driven bidirectional transport inside a cell is an intriguing phenomenon where multiple dyneins and kinesins drag cargoes and deliver them to specific locations. Quite often, both anterograde (plus) and retrograde (minus) directed motors reside simultaneously on the cargo and co-ordinate their activity such that the motion of the cargo looks like spells of plus directed and minus directed segments, with pauses in between. The stochastic tug-of-war model is widely used to analyze this motion, largely studied by computer simulations. Here, we present some analytical results for this model, such as the average durations of plus and minus-run segments and tug-of-war events as well as probabilities of persistence and reversal of directions following a tug-of-war. We show that cargo motion has memory in case of 2 dyneins and 1 kinesin driving together, while it is absent when only a dynein and a kinesin is involved.

  6. The Influence of Freight Trains on the Air Cargo in Kuwait International Airport

    Directory of Open Access Journals (Sweden)

    Fadala Hassan Alfadala

    2017-03-01

    Full Text Available Surrounded by countries who are moving continually towards economic development and stability, Kuwait has moved to establishing freight trains on the Air Cargo in Kuwait International Airport to meet the ever changing and increasing number of both passenger and cargoes going in and out of the country. As a member of the Gulf Cooperation Council or GCC it is clearly seen that as most of its member countries drive towards a collective effort to connect together by means of railroad networks, Kuwait’s effort to move transportation of cargoes to and from the Kuwait International Airport by linking with the GCC’s project to create railroad networks, the proposed ―Cargo City‖ becomes a major part of the realization of the development of the KIA which shall help with the aim to widen trade within and across GCC countries.

  7. 46 CFR 38.05-10 - Installation of cargo tanks-general-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... including testing for the hull and integral tanks. In the application of the requirements for the hydrostatic test of the cargo tanks, the hydrostatic test shall in no case be less severe than the...

  8. Hybridization of General Cargo Ships to meet the Required Energy Efficiency Design Index

    OpenAIRE

    Øverleir, Magnus Anders

    2015-01-01

    In this thesis a hybrid propulsion system is proposed for a general cargo ship with the aim to meet the required Energy Efficiency Design Index (EEDI). The study has investigated how a hybrid propulsion system will influence the ship s EEDI value and fuel economy. The central problem is the coming challenge for the general cargo segment meeting the required efficiency value. Especially small vessels (3 000-15 000 DWT) with high speed will have troubles complying with the stricter regulations....

  9. Experimental Research on Influence of Some Factors on Ejection Height of Cargo Projectile

    Institute of Scientific and Technical Information of China (English)

    郁浩; 王晓天; 李欣; 李纪敏

    2012-01-01

    Some factors influencing the cargo projectile's ejection height in different conditions were analyzed by using harmonic curve diagram and cluster method.The test results of the ejection height for a certain cargo projectile show that the wind speed and direction are the main influence factors.For given meteorological conditions,the applied environmental stress becomes the main cause,while the launching angle does not influence the ejection height.

  10. Design of a Large Span-Distributed Load Flying-Wing Cargo Airplane

    Science.gov (United States)

    Jernell, L. S.; Quartero, C. B.

    1977-01-01

    The design and operation of very large, long-range, subsonic cargo aircraft are considered. A design concept which distributes the payload along the wingspan to counterbalance the aerodynamic loads, with a resultant decrease in the in-flight wing bending moments and shear forces, is described. The decreased loading of the wing structure, coupled with the very thick wing housing the cargo, results in a relatively low overall structural weight in comparison to that of conventional aircraft.

  11. Underwater Photogrammetry and 3d Reconstruction of Marble Cargos Shipwreck

    Science.gov (United States)

    Balletti, C.; Beltrame, C.; Costa, E.; Guerra, F.; Vernier, P.

    2015-04-01

    Nowadays archaeological and architectural surveys are based on the acquisition and processing of point clouds, allowing a high metric precision, essential prerequisite for a good documentation. Digital image processing and laser scanner have changed the archaeological survey campaign, from manual and direct survey to a digital one and, actually, multi-image photogrammetry is a good solution for the underwater archaeology. This technical documentation cannot operate alone, but it has to be supported by a topographical survey to georeference all the finds in the same reference system. In the last years the Ca' Foscari and IUAV University of Venice are conducting a research on integrated survey techniques to support underwater metric documentation. The paper will explain all the phases regarding the survey's design, images acquisition, topographic measure and the data processing of two Roman shipwrecks in south Sicily. The cargos of the shipwrecks are composed by huge marble blocks, but they are different for morphological characteristic of the sites, for the depth and for their distribution on the seabed. Photogrammetrical and topographical surveys were organized in two distinct methods, especially for the second one, due to the depth that have allowed an experimentation of GPS RTK's measurements on one shipwreck. Moreover, this kind of three-dimensional documentation is useful for educational and dissemination aspect, for the ease of understanding by wide public.

  12. Mulheres em cargos profissionais de chefia: o paradoxo da igualdade

    Directory of Open Access Journals (Sweden)

    Fabia Alberton de Silva Galvane

    2015-12-01

    Full Text Available Resumo O artigo aqui apresentado foi construído a partir de informações obtidas em uma pesquisa realizada no ano de 2009, apresentada como Trabalho de Conclusão de Curso da Psicologia, ao Centro Universitário Barriga Verde - UNIBAVE (GALVANE, 2009. A pesquisa teve como objetivo geral verificar os sentidos produzidos por mulheres que ocupam cargos de chefia em indústrias de uma cidade do sul de Santa Catarina, Brasil. O modelo de pesquisa foi o qualitativo proposto por González Rey (2005. Os sujeitos da pesquisa foram sete mulheres e as informações obtidas por meio de entrevistas semiestruturadas. Neste artigo, propõe-se revisar informações obtidas e ampliar discussões outrora realizadas, utilizando-se das contribuições teóricas de Joan Scott e Judith Butler. Entre outras questões relevantes, o estudo sugere que as entrevistadas utilizam concepções baseadas em “certa essência feminina” como estratégia de visibilidade, permanência e poder no mercado de trabalho.

  13. Multivalent viral capsids with internal cargo for fibrin imaging.

    Directory of Open Access Journals (Sweden)

    Allie C Obermeyer

    Full Text Available Thrombosis is the cause of many cardiovascular syndromes and is a significant contributor to life-threatening diseases, such as myocardial infarction and stroke. Thrombus targeted imaging agents have the capability to provide molecular information about pathological clots, potentially improving detection, risk stratification, and therapy of thrombosis-related diseases. Nanocarriers are a promising platform for the development of molecular imaging agents as they can be modified to have external targeting ligands and internal functional cargo. In this work, we report the synthesis and use of chemically functionalized bacteriophage MS2 capsids as biomolecule-based nanoparticles for fibrin imaging. The capsids were modified using an oxidative coupling reaction, conjugating ∼90 copies of a fibrin targeting peptide to the exterior of each protein shell. The ability of the multivalent, targeted capsids to bind fibrin was first demonstrated by determining the impact on thrombin-mediated clot formation. The modified capsids out-performed the free peptides and were shown to inhibit clot formation at effective concentrations over ten-fold lower than the monomeric peptide alone. The installation of near-infrared fluorophores on the interior surface of the capsids enabled optical detection of binding to fibrin clots. The targeted capsids bound to fibrin, exhibiting higher signal-to-background than control, non-targeted MS2-based nanoagents. The in vitro assessment of the capsids suggests that fibrin-targeted MS2 capsids could be used as delivery agents to thrombi for diagnostic or therapeutic applications.

  14. El sistema de cargos en San Bernardino de Milpillas Chico, Durango

    Directory of Open Access Journals (Sweden)

    Chantal Cramaussel

    2013-01-01

    Full Text Available La principal estructura política de los tepehuanos es el sistema de cargos. En San Bernardino de Milpillas, Durango, los cargos de la Iglesia son diferentes a los del gobierno tradicional. Los segundos parecen apegarse más a las culturales locales, aunque los cargos de la Iglesia han sido objeto de transformaciones significativas para excluir a los ministros católicos en las ceremonias cristianas. La fiesta de la Candelaria, que precede a la toma de posesión de los nuevos “empleados”, muestra que los aspectos civiles de los cargos son indisociables de los rituales religiosos. Los cargos reunían funciones militares, judiciales y económicas, pero éstas han ido perdiendo vigencia por la violencia y por la compañía forestal, una notable fuente de recursos. Sin embargo, por el sistema de cargos los mestizos de Milpillas se han integrado a la organización política y la cultura de los tepehuanos.

  15. External forces influence the elastic coupling effects during cargo transport by molecular motors.

    Science.gov (United States)

    Berger, Florian; Keller, Corina; Klumpp, Stefan; Lipowsky, Reinhard

    2015-02-01

    Cellular transport is achieved by the cooperative action of molecular motors which are elastically linked to a common cargo. When the motors pull on the cargo at the same time, they experience fluctuating elastic strain forces induced by the stepping of the other motors. These elastic coupling forces can influence the motors' stepping and unbinding behavior and thereby the ability to transport cargos. Based on a generic single motor description, we introduce a framework that explains the response of two identical molecular motors to a constant external force. In particular, we relate the single motor parameters, the coupling strength and the external load force to the dynamics of the motor pair. We derive four distinct transport regimes and determine how the crossover lines between the regimes depend on the load force. Our description of the overall cargo dynamics takes into account relaxational displacements of the cargo caused by the unbinding of one motor. For large forces and weak elastic coupling these back-shifts dominate the displacements. To develop an intuitive understanding about motor cooperativity during cargo transport, we introduce a time scale for load sharing. This time scale allows us to predict how the regulation of single motor parameters influences the cooperativity. As an example, we show that up-regulating the single motor processivity enhances load sharing of the motor pair.

  16. Irradiation Effects for the Pulsed Fast Neutron Analysis (PFNA) Cargo Interrogation System

    Energy Technology Data Exchange (ETDEWEB)

    Slater, C.O.

    2001-02-02

    At the request of Safety and Ecology Corporation of Tennessee, radiation effects of the proposed Pulsed Fast Neutron Analysis (PFNA) Cargo Interrogation System have been examined. First, fissile cargo were examined to determine if a significant neutron signal would be observable during interrogation. Results indicated that ample multiplication would be seen for near critical bare targets. The water-reflected sphere showed relatively little multiplication. By implication, a fissile target shielded by hydrogenous cargo might not be detectable by neutron interrogation, particularly if reliance is placed on the neutron signal. The cargo may be detectable if use can be made of the ample increase in the photon signal. Second, dose rates were calculated at various locations within and just outside the facility building. These results showed that some dose rates may be higher than the target dose rate of 0.05 mrem/h. However, with limited exposure time, the total dose may be well below the allowed total dose. Lastly, estimates were made of the activation of structures and typical cargo. Most cargo will not be exposed long enough to be activated to levels of concern. On the other hand, portions of the structure may experience buildup of some radionuclides to levels of concern.

  17. Cargo-towing synthetic nanomachines: towards active transport in microchip devices.

    Science.gov (United States)

    Wang, Joseph

    2012-05-08

    This review article discusses the use of synthetic catalytic nano motors for cargo manipulations and for developing miniaturized lab-on-chip systems based on autonomous transport. The ability of using chemically-powered artificial nanomotors to capture, transport and release therapeutic payloads or nanostructured biomaterials represents one of the next major prospects for nanomotor development. The increased cargo-towing force of such self-propelled nanomotors, along with their precise motion control within microchannel networks, versatility and facile functionalization, pave the way to new integrated functional lab-on-a-chip powered by active transport and perform a series of tasks. Such use of cargo-towing artificial nanomotors has been inspired by on-chip kinesin molecular shuttles. Functionalized nano/microscale motors can thus be used to pick a selected nano/microscale chemical or biological payload target at the right place, transport and deliver them to a target location in a timely manner. Key challenges for using synthetic nanomachines for driving transport processes along microchannel networks are discussed, including loading and unloading of cargo and precise motion control, along with recent examples of related cargo manipulation processes and guided transport in lab-on-a-chip formats. The exciting research area of cargo-carrying catalytic man-made nanomachines is expected to grow rapidly, to lead to new lab-on-a-chip formats and to provide a wide range of future microchip opportunities.

  18. NanoShuttles: Harnessing Motor Proteins to Transport Cargo in Synthetic Environments

    Science.gov (United States)

    Vogel, V.; Hess, H.

    Motors have become a crucial commodity in our daily lives, from transportation to driving conveyor belts that enable the sequential assembly of cars and other industrial machines. For the sequential assembly of building blocks at the nanoscale that would not assemble spontaneously into larger functional systems, however, active transport systems are not yet available. In contrast, cells have evolved sophisticated molecular machinery that drives movement and active transport. Driven by the conversion of chemical into mechanical energy, namely through hydrolysis of the biological fuel ATP, molecular motors enable cells to operate far away from equilibrium by transporting organelles and molecules to designated locations within the cell, often against concentration gradients. Inspired by the biological concept of active transport, major efforts are underway to learn how to build nanoscale transport systems that are driven by molecular motors. Emerging engineering principles are discussed of how to build tracks and junctions to guide such nanoshuttles, how to load them with cargo and control their speed, how to use active transport to assemble mesoscopic structures that would otherwise not assemble spontaneously and what polymeric materials to choose to integrate motors into MEMS and other biohybrid devices. Finally, two applications that exploit the physical properties of microtubules are discussed, surface imaging by a swarm of microtubules and a self-assembled picoNewton force meter to probe receptor-ligand interactions.

  19. Novel propulsion of active colloids by self-induced field gradients with potential for cargo transport

    Science.gov (United States)

    Boymelgreen, Alicia; Yossifon, Gilad; Miloh, Touvia

    2016-11-01

    Localized electric field gradients, induced by the dual symmetry-breaking of an asymmetric particle adjacent to a wall are shown to potentially drive particle motion, even in a uniform field. Since the driving gradient is induced by the particle itself, we have termed this propulsion mechanism "self-dielectrophoresis" (sDEP), to distinguish from traditional DEP where the driving non-uniform field is externally fixed and particle direction is restricted. It is also shown that sDEP driven particles are natural cargo carriers, since the localized gradients can also trap and release targets selectively and on demand. This phenomenon is specifically characterized for Gold-Polystyrene Janus spheres, including the establishment of a non-dimensional parameter marking the critical frequency at which sDEP dominates low-frequency ICEP- evidenced by a reversal in particle direction. Additionally we demonstrate that localized gradients can transform the translating Janus particles into an externally controlled, mobile floating electrode with the ability to collect, transport and release a target sample a target 1/50 of its size. It is also shown that calculated control of the frequency enables selective sorting and transport - if the driving frequency is aligned with the positive-DEP (pDEP) response of a specific "target" and negative-DEP (nDEP) of any other contaminants, only the former will be transported with the Janus sphere. ISF,BSF,RBNI.

  20. Air cargo market outlook and impact via the NASA CLASS project. [Cargo/Logistics Airlift Systems Study

    Science.gov (United States)

    Winston, M. M.; Conner, D. W.

    1980-01-01

    An overview is given of the Cargo/Logistics Airlift Systems Study (CLASS) project which was a 10 man-year effort carried out by two contractor teams, aimed at defining factors impacting future system growth and obtaining market requirements and design guidelines for future air freighters. Growth projection was estimated by two approaches: one, an optimal systems approach with a more efficient and cost effective system considered as being available in 1990; and the other, an evolutionary approach with an econometric behavior model used to predict long term evolution from the present system. Both approaches predict significant growth in demand for international air freighter services and less growth for U.S. domestic services. Economic analysis of air freighter fleet options indicate very strong market appeal of derivative widebody transports in 1990 with little incentive to develop all new dedicated air freighters utilizing the 1990's technology until sometime beyond the year 2000. Advanced air freighters would be economically attractive for a wide range of payload sizes (to 500 metric tons), however, if a government would share in the RD and T costs by virtue of its needs for a slightly modified version of a civil air freighter design (a.g. military airlifter).

  1. Large area x-ray detectors for cargo radiography

    Science.gov (United States)

    Bueno, C.; Albagli, D.; Bendahan, J.; Castleberry, D.; Gordon, C.; Hopkins, F.; Ross, W.

    2007-04-01

    Large area x-ray detectors based on phosphors coupled to flat panel amorphous silicon diode technology offer significant advances for cargo radiologic imaging. Flat panel area detectors provide large object coverage offering high throughput inspections to meet the high flow rate of container commerce. These detectors provide excellent spatial resolution when needed, and enhanced SNR through low noise electronics. If the resolution is reduced through pixel binning, further advances in SNR are achievable. Extended exposure imaging and frame averaging enables improved x-ray penetration of ultra-thick objects, or "select-your-own" contrast sensitivity at a rate many times faster than LDAs. The areal coverage of flat panel technology provides inherent volumetric imaging with the appropriate scanning methods. Flat panel area detectors have flexible designs in terms of electronic control, scintillator selection, pixel pitch, and frame rates. Their cost is becoming more competitive as production ramps up for the healthcare, nondestructive testing (NDT), and homeland protection industries. Typically used medical and industrial polycrystalline phosphor materials such as Gd2O2S:Tb (GOS) can be applied to megavolt applications if the phosphor layer is sufficiently thick to enhance x-ray absorption, and if a metal radiator is used to augment the quantum detection efficiency and reduce x-ray scatter. Phosphor layers ranging from 0.2-mm to 1-mm can be "sandwiched" between amorphous silicon flat panel diode arrays and metal radiators. Metal plates consisting of W, Pb or Cu, with thicknesses ranging from 0.25-mm to well over 1-mm can be used by covering the entire area of the phosphor plate. In some combinations of high density metal and phosphor layers, the metal plate provides an intensification of 25% in signal due to electron emission from the plate and subsequent excitation within the phosphor material. This further improves the SNR of the system.

  2. FAM21 directs SNX27-retromer cargoes to the plasma membrane by preventing transport to the Golgi apparatus.

    Science.gov (United States)

    Lee, Seongju; Chang, Jaerak; Blackstone, Craig

    2016-03-09

    The endosomal network maintains cellular homeostasis by sorting, recycling and degrading endocytosed cargoes. Retromer organizes the endosomal sorting pathway in conjunction with various sorting nexin (SNX) proteins. The SNX27-retromer complex has recently been identified as a major endosomal hub that regulates endosome-to-plasma membrane recycling by preventing lysosomal entry of cargoes. Here, we show that SNX27 directly interacts with FAM21, which also binds retromer, within the Wiskott-Aldrich syndrome protein and SCAR homologue (WASH) complex. This interaction is required for the precise localization of SNX27 at an endosomal subdomain as well as for recycling of SNX27-retromer cargoes. Furthermore, FAM21 prevents cargo transport to the Golgi apparatus by controlling levels of phosphatidylinositol 4-phosphate, which facilitates cargo dissociation at the Golgi. Together, our results demonstrate that the SNX27-retromer-WASH complex directs cargoes to the plasma membrane by blocking their transport to lysosomes and the Golgi.

  3. Spiders (Araneae) Found in Bananas and Other International Cargo Submitted to North American Arachnologists for Identification.

    Science.gov (United States)

    Vetter, Richard S; Crawford, Rodney L; Buckle, Donald J

    2014-11-01

    Spiders found in international cargo brought into North America are sometimes submitted to arachnologists for identification. Often, these spiders are presumed to be of medical importance because of size or a submitter's familiarity with a toxic spider genus from the continent of origin. Starting in 2006, requests were made for spiders found in international cargo brought into North America, in addition to the specimens from similar cargo shipments already in our museum collections. This was an ad hoc study that allowed us to focus on spiders of concern to the discoverer. We identified 135 spiders found in international cargo. A key for the most common species is provided. The most frequently submitted spiders were the pantropical huntsman spider, Heteropoda venatoria (L.) (Sparassidae), and the redfaced banana spider, Cupiennius chiapanensis Medina Soriano (Ctenidae). Spiders of medical importance were rare. The most common cargo from which spiders were submitted was bananas with most specimens coming from Central America, Ecuador, or Colombia. Lack of experience with nonnative fauna caused several experienced American arachnologists to misidentify harmless ctenid spiders (C. chiapanensis, spotlegged banana spider, Cupiennius getazi Simon) as highly toxic Phoneutria spiders. These misidentifications could have led to costly, unwarranted prophylactic eradication measures, unnecessary employee health education, heightened employee anxiety and spoilage when perishable goods are left unloaded due to safety concerns.

  4. LUCID: A Quantitative Assay of ESCRT-Mediated Cargo Sorting into Multivesicular Bodies.

    Science.gov (United States)

    Nickerson, Daniel P; Merz, Alexey J

    2015-12-01

    Endosomes are transportation nodes, mediating selective transport of soluble and transmembrane cargos to and from the Golgi apparatus, plasma membrane and lysosomes. As endosomes mature to become multivesicular bodies (MVBs), Endosomal Sorting Complexes Required for Transport (ESCRTs) selectively incorporate transmembrane cargos into vesicles that bud into the endosome lumen. Luminal vesicles and their cargoes are targeted for destruction when MVBs fuse with lysosomes. Common assays of endosomal luminal targeting, including fluorescence microscopy and monitoring of proteolytic cargo maturation, possess significant limitations. We present a quantitative assay system called LUCID (LUCiferase reporter of Intraluminal Deposition) that monitors exposure of chimeric luciferase-cargo reporters to cytosol. Luciferase-chimera signal increases when sorting to the endosome lumen is disrupted, and silencing of signal from the chimera depends upon luminal delivery of the reporter rather than proteolytic degradation. The system presents several advantages, including rapidity, microscale operation and a high degree of reproducibility that enables detection of subtle phenotypic differences. Luciferase reporters provide linear signal over an extremely broad dynamic range, allowing analysis of reporter traffic even at anemic levels of expression. Furthermore, LUCID reports transport kinetics when applied to inducible trafficking reporters.

  5. Molecular motors pulling cargos in the viscoelastic cytosol: how power strokes beat subdiffusion.

    Science.gov (United States)

    Goychuk, Igor; Kharchenko, Vasyl O; Metzler, Ralf

    2014-08-21

    The discovery of anomalous diffusion of larger biopolymers and submicron tracers such as endogenous granules, organelles, or virus capsids in living cells, attributed to the viscoelastic nature of the cytoplasm, provokes the question whether this complex environment equally impacts the active intracellular transport of submicron cargos by molecular motors such as kinesins: does the passive anomalous diffusion of free cargo always imply its anomalously slow active transport by motors, the mean transport distance along microtubule growing sublinearly rather than linearly in time? Here we analyze this question within the widely used two-state Brownian ratchet model of kinesin motors based on the continuous-state diffusion along microtubules driven by a flashing binding potential, where the cargo particle is elastically attached to the motor. Depending on the cargo size, the loading force, the amplitude of the binding potential, the turnover frequency of the molecular motor enzyme, and the linker stiffness we demonstrate that the motor transport may turn out either normal or anomalous, as indeed measured experimentally. We show how a highly efficient normal active transport mediated by motors may emerge despite the passive anomalous diffusion of the cargo, and study the intricate effects of the elastic linker. Under different, well specified conditions the microtubule-based motor transport becomes anomalously slow and thus significantly less efficient.

  6. Drive the Car(go)s—New Modalities to Control Cargo Trafficking in Live Cells

    Science.gov (United States)

    Mondal, Payel; Khamo, John S.; Krishnamurthy, Vishnu V.; Cai, Qi; Zhang, Kai

    2017-01-01

    Synaptic transmission is a fundamental molecular process underlying learning and memory. Successful synaptic transmission involves coupled interaction between electrical signals (action potentials) and chemical signals (neurotransmitters). Defective synaptic transmission has been reported in a variety of neurological disorders such as Autism and Alzheimer’s disease. A large variety of macromolecules and organelles are enriched near functional synapses. Although a portion of macromolecules can be produced locally at the synapse, a large number of synaptic components especially the membrane-bound receptors and peptide neurotransmitters require active transport machinery to reach their sites of action. This spatial relocation is mediated by energy-consuming, motor protein-driven cargo trafficking. Properly regulated cargo trafficking is of fundamental importance to neuronal functions, including synaptic transmission. In this review, we discuss the molecular machinery of cargo trafficking with emphasis on new experimental strategies that enable direct modulation of cargo trafficking in live cells. These strategies promise to provide insights into a quantitative understanding of cargo trafficking, which could lead to new intervention strategies for the treatment of neurological diseases. PMID:28163671

  7. Cargo binding promotes KDEL receptor clustering at the mammalian cell surface.

    Science.gov (United States)

    Becker, Björn; Shaebani, M Reza; Rammo, Domenik; Bubel, Tobias; Santen, Ludger; Schmitt, Manfred J

    2016-06-29

    Transmembrane receptor clustering is a ubiquitous phenomenon in pro- and eukaryotic cells to physically sense receptor/ligand interactions and subsequently translate an exogenous signal into a cellular response. Despite that receptor cluster formation has been described for a wide variety of receptors, ranging from chemotactic receptors in bacteria to growth factor and neurotransmitter receptors in mammalian cells, a mechanistic understanding of the underlying molecular processes is still puzzling. In an attempt to fill this gap we followed a combined experimental and theoretical approach by dissecting and modulating cargo binding, internalization and cellular response mediated by KDEL receptors (KDELRs) at the mammalian cell surface after interaction with a model cargo/ligand. Using a fluorescent variant of ricin toxin A chain as KDELR-ligand (eGFP-RTA(H/KDEL)), we demonstrate that cargo binding induces dose-dependent receptor cluster formation at and subsequent internalization from the membrane which is associated and counteracted by anterograde and microtubule-assisted receptor transport to preferred docking sites at the plasma membrane. By means of analytical arguments and extensive numerical simulations we show that cargo-synchronized receptor transport from and to the membrane is causative for KDELR/cargo cluster formation at the mammalian cell surface.

  8. 46 CFR 151.25-1 - Cargo tank.

    Science.gov (United States)

    2010-10-01

    ... continuously swept with air by means of blowers or other mechanical devices requiring power. (d) Ventilated... without the use of blowers or other mechanical devices requiring power (e.g., “chimney-effect”...

  9. Electrostatic assembly/disassembly of nanoscaled colloidosomes for light-triggered cargo release

    KAUST Repository

    Li, Song

    2015-04-27

    Colloidosome capsules possess the potential for the encapsulation and release of molecular and macromolecular cargos. However, the stabilization of the colloidosome shell usually requires an additional covalent crosslinking which irreversibly seals the capsules, and greatly limits their applications in large-cargos release. Herein we report nanoscaled colloidosomes designed by the electrostatic assembly of organosilica nanoparticles (NPs) with oppositely charged surfaces (rather than covalent bonds), arising from different contents of a bridged nitrophenylene-alkoxysilane [NB; 3-nitro-N-(3-(triethoxysilyl)propyl)-4-(((3-(triethoxysilyl)propyl)-amino)methyl)benzamid] derivative in the silica. The surface charge of the positively charged NPs was reversed by light irradiation because of a photoreaction in the NB moieties, which impacted the electrostatic interactions between NPs and disassembled the colloidosome nanosystems. This design was successfully applied for the encapsulation and light-triggered release of cargos. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Binding to any ESCRT can mediate ubiquitin-independent cargo sorting

    Science.gov (United States)

    Mageswaran, Shrawan Kumar; Dixon, Megan Gorringe; Curtiss, Matt; Keener, James P.; Babst, Markus

    2013-01-01

    The ESCRT machinery is known to sort ubiquitinated transmembrane proteins into vesicles that bud into the lumen of multivesicular bodies (MVBs). Although the ESCRTs themselves are ubiquitinated they are excluded from the intraluminal vesicles and recycle back to the cytoplasm for further rounds of sorting. To obtain insights into the rules that distinguish ESCRT machinery from cargo we analyzed the trafficking of artificial ESCRT-like protein fusions. These studies showed that lowering ESCRT-binding affinity converts a protein from behaving like ESCRT machinery into cargo of the MVB pathway, highlighting the close relationship between machinery and the cargoes they sort. Furthermore, our findings give insights into the targeting of soluble proteins into the MVB pathway and show that binding to any of the ESCRTs can mediate ubiquitin-independent MVB sorting. PMID:24148098

  11. Dry bulk cargo shipping - An overlooked threat to the marine environment?

    Science.gov (United States)

    Grote, Matthias; Mazurek, Nicole; Gräbsch, Carolin; Zeilinger, Jana; Le Floch, Stéphane; Wahrendorf, Dierk-Steffen; Höfer, Thomas

    2016-09-15

    Approximately 9.5billiontonnes of goods is transported over the world oceans annually with dry bulk representing the largest cargo group. This paper aims to analyse whether the transport and associated inputs of dry bulks into the sea create a risk for the marine environment. For this purpose, we analyse the international regulatory background concerning environmental protection (MARPOL), estimate quantities and identify inputs of such cargoes into the oceans (accidental and operational), and use available information for hazard assessment. Annually, more than 2.15milliontonnes of dry bulk cargoes are likely to enter the oceans, of which 100,000tonnes are potentially harmful to the marine environment according to the definition included in draft maritime regulation. The assessment of the threat to the marine environment is hampered by a lack of available information on chemical composition, bioavailability and toxicity. Perspectives for amendments of the unsatisfying pollution prevention regulations are discussed.

  12. Solar-electric-propulsion cargo vehicles for split/sprint Mars mission

    Science.gov (United States)

    Callaghan, Christopher E.; Crowe, Michael D.; Swis, Matthew J.; Mickney, Marcus R.; Montgomery, C. Keith; Walters, Robert; Thoden, Scott

    1991-01-01

    In support of the proposed exploration of Mars, an unmanned cargo ferry SEMM1 (Solar Electric Mars Mission) was designed. The vehicle is based on solar electric propulsion, and required to transport a cargo of 61,000 kg. The trajectory is a combination of spirals; first, out from LEO, then around the sun, then spiral down to low Mars orbit. The spacecraft produces 3.03 MWe power using photovoltaic flexible blanket arrays. Ion thrusters using argon as a propellant were selected to drive the ship, providing about 60 Newtons of thrust in low Earth orbit. The configuration is based on two long truss beams to which the 24 individual, self-deployable, solar arrays are attached. The main body module supports the two beams and houses the computers, electrical, and control equipment. The thruster module is attached to the rear of the main body, and the cargo to the front.

  13. An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor

    Science.gov (United States)

    Dagdas, Yasin F; Belhaj, Khaoula; Maqbool, Abbas; Chaparro-Garcia, Angela; Pandey, Pooja; Petre, Benjamin; Tabassum, Nadra; Cruz-Mireles, Neftaly; Hughes, Richard K; Sklenar, Jan; Win, Joe; Menke, Frank; Findlay, Kim; Banfield, Mark J; Kamoun, Sophien; Bozkurt, Tolga O

    2016-01-01

    Plants use autophagy to safeguard against infectious diseases. However, how plant pathogens interfere with autophagy-related processes is unknown. Here, we show that PexRD54, an effector from the Irish potato famine pathogen Phytophthora infestans, binds host autophagy protein ATG8CL to stimulate autophagosome formation. PexRD54 depletes the autophagy cargo receptor Joka2 out of ATG8CL complexes and interferes with Joka2's positive effect on pathogen defense. Thus, a plant pathogen effector has evolved to antagonize a host autophagy cargo receptor to counteract host defenses. DOI: http://dx.doi.org/10.7554/eLife.10856.001 PMID:26765567

  14. Design and development of a focusing electromagnet for RF linac of a cargo scanner

    Energy Technology Data Exchange (ETDEWEB)

    Nayak, B., E-mail: biswaranjan.nayak1@gmail.com; Acharya, S.; Bhattacharjee, D.; Chandan, Shiv; Choudhury, N.; Sharma, V.; Mittal, K.C.; Gantayet, L.M.

    2014-11-11

    Cargo scanning of contraband objects by high energetic X-rays requires compact and self-contained RF linacs. To achieve this goal, a 6 MeV, 700 W standing wave RF electron linac has been developed at Accelerator and Pulse Power Division, BARC. To obtain the image of scanned objects with good resolution requires a focused electron beam at the target. This paper presents the design, development and integration of a solenoid magnet with a compact RF linac and its performance with electron beam for cargo scanning applications.

  15. Air cargo: An Integrated Systems View. 1978 Summer Faculty Fellowship Program in Engineering Systems Design

    Science.gov (United States)

    Keaton, A. (Editor); Eastman, R. (Editor); Hargrove, A. (Editor); Rabiega, W. (Editor); Olsen, R. (Editor); Soberick, M. (Editor)

    1978-01-01

    The national air cargo system is analyzed and how it should be in 1990 is prescribed in order to operate successfully through 2015; that is through one equipment cycle. Elements of the system which are largely under control of the airlines and the aircraft manufacturers are discussed. The discussion deals with aircraft, networks, facilities, and procedures. The regulations which govern the movement of air freight are considered. The larger public policy interests which must be served by the kind of system proposed, the air cargo integrated system (ACIS), are addressed. The possible social, economical, political, and environment impacts of the system are considered. Recommendations are also given.

  16. Enabling a viable technique for the optimization of LNG carrier cargo operations

    Science.gov (United States)

    Alaba, Onakoya Rasheed; Nwaoha, T. C.; Okwu, M. O.

    2016-09-01

    In this study, we optimize the loading and discharging operations of the Liquefied Natural Gas (LNG) carrier. First, we identify the required precautions for LNG carrier cargo operations. Next, we prioritize these precautions using the analytic hierarchy process (AHP) and experts' judgments, in order to optimize the operational loading and discharging exercises of the LNG carrier, prevent system failure and human error, and reduce the risk of marine accidents. Thus, the objective of our study is to increase the level of safety during cargo operations.

  17. Enabling a Viable Technique for the Optimization of LNG Carrier Cargo Operations

    Institute of Scientific and Technical Information of China (English)

    Onakoya Rasheed Alaba; T C Nwaoha; M O Okwu

    2016-01-01

    In this study, we optimize the loading and discharging operations of the Liquefied Natural Gas (LNG) carrier. First, we identify the required precautions for LNG carrier cargo operations. Next, we prioritize these precautions using the analytic hierarchy process (AHP) and experts’ judgments, in order to optimize the operational loading and discharging exercises of the LNG carrier, prevent system failure and human error, and reduce the risk of marine accidents. Thus, the objective of our study is to increase the level of safety during cargo operations.

  18. Protocells and their use for targeted delivery of multicomponent cargos to cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Brinker, Jeffrey C.; Ashley, Carlee Erin; Jiang, Xingmao; Liu, Juewen; Peabody, David S.; Wharton, Walker Richard; Carnes, Eric; Chackerian, Bryce; Willman, Cheryl L.

    2016-11-01

    Various embodiments provide materials and methods for synthesizing protocells for use in targeted delivery of cargo components to cancer cells. In one embodiment, the lipid bilayer can be fused to the porous particle core to form a protocell. The lipid bilayer can be modified with targeting ligands or other ligands to achieve targeted delivery of cargo components that are loaded within the protocell to a target cell, e.g., a type of cancer. Shielding materials can be conjugated to the surface of the lipid bilayer to reduce undesired non-specific binding.

  19. Boeing and EGAT Unveil World's First Large Cargo Freighter——Evergreen Aviation Technologies transforms Boeing747-400 for 787 Dreamliner cargo

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Evergreen Aviation Technologies Corporation (EGAT), a subsidiary of the Evergreen Group, and The Boeing Company unveiled the first of three Boeing 747-400 Large Cargo Freighters (LCF) modified to exacting specifications to transport giant-sized Boeing 787 "Dreamliner" assemblages.Boeing selected EGAT to perform the specialized aircraft conversions and work began in 2005. The three-aircraft conversion program will be completed in 2007.

  20. 77 FR 11145 - Intent to Request Renewal From OMB of One Current Public Collection of Information: Air Cargo...

    Science.gov (United States)

    2012-02-24

    ... Collection of Information: Air Cargo Security Requirements AGENCY: Transportation Security Administration...-cargo carriers. The collections of information that make up this ICR are security programs, security threat assessments (STA), known shipper data via the Known Shipper Management System (KSMS), Air...

  1. Triple Emulsion Drops with An Ultrathin Water Layer: High Encapsulation Efficiency and Enhanced Cargo Retention in Microcapsules.

    Science.gov (United States)

    Choi, Chang-Hyung; Lee, Hyomin; Abbaspourrad, Alireza; Kim, June Hwan; Fan, Jing; Caggioni, Marco; Wesner, Chris; Zhu, Taotao; Weitz, David A

    2016-05-01

    Triple emulsion drops with an ultrathin water layer are developed to achieve high encapsulation efficiency of hydrophobic cargo in a hydrophobic polymeric shell, directly dispersed in water. Furthermore, enhanced retention of volatile hydrophobic cargo is achieved by forming a hydrogel network within this water layer that serves as a physical barrier.

  2. 19 CFR 103.31a - Advance electronic information for air, truck, and rail cargo; Importer Security Filing...

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Advance electronic information for air, truck, and rail cargo; Importer Security Filing information for vessel cargo. 103.31a Section 103.31a Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE...

  3. Overview of Crane Control Systems and the Related Problems: Analysis of Container Oscillation Using Different Types of Cargoes

    Directory of Open Access Journals (Sweden)

    Tomas Eglynas

    2016-04-01

    Full Text Available Growing international trading increased cargo transportation in containers, therefore the port cranes have higher loads. Increased cargo flows can influence transportation safety. It is therefore necessary to review the crane systems and determine what factors might influence the volatility of the container and its cargo during transportation. The paper includes consideration and analysis of crane control systems and related problems. The authors consider the reasons of problems, probable damage and solution methods. The paper also provides the analysis of the relationship between different container cargoes and container oscillations occurring during handling operations using a container crane prototype. The analysis of the effect of different cargoes in containers on loading process and the results of occurring oscillations are presented.

  4. 46 CFR 32.50-1 - Cargo pumps for tank vessels constructed on or after November 10, 1936-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo pumps for tank vessels constructed on or after... TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Pumps, Piping, and Hose for Cargo Handling § 32.50-1 Cargo pumps for tank vessels constructed on or after November 10, 1936—TB/ALL. On...

  5. 46 CFR 32.50-25 - Cargo pumps and piping on tank vessels constructed prior to November 10, 1936-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo pumps and piping on tank vessels constructed prior... SECURITY TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Pumps, Piping, and Hose for Cargo Handling § 32.50-25 Cargo pumps and piping on tank vessels constructed prior to November 10,...

  6. Analysis of VGH data from two types of four-engine airplanes in commercial cargo service

    Science.gov (United States)

    Healy, F. M.

    1972-01-01

    Data are presented for derived gust velocities and for incremental normal accelerations due to gusts, maneuvers, and landing impacts. The data were obtained from NASA VGH recorders installed on three four-engine cargo airplanes operated by three airlines. Continental United States and trans-Pacific routes were covered.

  7. 19 CFR 122.48a - Electronic information for air cargo required in advance of arrival.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Electronic information for air cargo required in... OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Entry and Entry Documents; Electronic Manifest Requirements for Passengers, Crew Members, and Non-Crew Members...

  8. 49 CFR 173.33 - Hazardous materials in cargo tank motor vehicles.

    Science.gov (United States)

    2010-10-01

    ... is marked and used exclusively to transport carbon dioxide, or except when an MC 338 is used to transport argon, carbon dioxide, helium, krypton, neon, nitrogen, and xenon. However, if the cargo tank... that provides the venting capacity prescribed in § 178.345-10(e) of this subchapter. The...

  9. 76 FR 57941 - Retrospective Review Under E.O. 13563: Cargo Preference

    Science.gov (United States)

    2011-09-19

    ... public meeting will be broadcast live via Web streaming and a listen-only telephone line. The public may access the live Web streaming by a link from http://www.marad.dot.gov . Listen-only telephone line... assessing the cargo preference regulatory process. The recorded webcast video will remain...

  10. 14 CFR 23.855 - Cargo and baggage compartment fire protection.

    Science.gov (United States)

    2010-01-01

    ... Design and Construction Fire Protection § 23.855 Cargo and baggage compartment fire protection. (a... protection. 23.855 Section 23.855 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... the contents of a hand held fire extinguisher, or (2) Be equipped with a smoke or fire detector...

  11. 76 FR 13313 - Hazardous Materials: Cargo Tank Motor Vehicle Loading and Unloading Operations

    Science.gov (United States)

    2011-03-11

    ... cargo transfer hoses on an adjacent tank car resulted in the release of chlorine. Three plant employees... of the explosion propelled a 300-pound tank car dome housing about \\1/3\\ mile away from the tank car... repaired fill hose and began to unload the gas from his 8,000-gallon tanker truck. In less than a...

  12. 77 FR 9916 - California State Motor Vehicle and Nonroad Engine Pollution Control Standards; Mobile Cargo...

    Science.gov (United States)

    2012-02-21

    ... AGENCY California State Motor Vehicle and Nonroad Engine Pollution Control Standards; Mobile Cargo... to the control of emissions from new motor vehicles or new motor vehicle engines ] EPA is, pursuant... standards relating to the control of emissions for new motor vehicles and new motor vehicle...

  13. Indications of marine bioinvasion from network theory. An analysis of the global cargo ship network

    NARCIS (Netherlands)

    Kölzsch, A.; Blasius, B.

    2011-01-01

    The transport of huge amounts of small aquatic organisms in the ballast tanks and at the hull of large cargo ships leads to ever increasing rates of marine bioinvasion. In this study, we apply a network theoretic approach to examine the introduction of invasive species into new ports by global shipp

  14. Cargo Revenue Management: Bid-Prices for a 0-1 Multi Knapsack Problem

    NARCIS (Netherlands)

    K. Pak; R. Dekker (Rommert)

    2004-01-01

    textabstractRevenue management is the practice of selecting those customers that generate the maximum revenue from a fixed and perishable capacity. Cargo revenue management differs from the well-known passenger revenue management problem by the fact that its capacity constraint is 2-dimensional, i.e

  15. Rail freight vibration impact sleep and community response: An overview of CargoVibes

    NARCIS (Netherlands)

    Persson Waye, K.; Janssen, S.A.; Waddington, D.; Groll, W.; Croy, I.; Hammar, O.; Koopman, A.; Moorhouse, A.; Peris, E.; Sharp, C.; Sica, G.; Smith, M.G.; Vos, H.; Woodcock, J.; Ogren, M.

    2014-01-01

    The European Union funded project: CargoVibes involving ten partners from eight nations has aimed to examine ground-borne vibration affecting residents close to freight railway lines. The paper presents an overview of the work package investigating human response to vibration, with particular focus

  16. Plasmodium falciparum Sec24 marks transitional ER that exports a model cargo via a diacidic motif.

    Science.gov (United States)

    Lee, Marcus C S; Moura, Pedro A; Miller, Elizabeth A; Fidock, David A

    2008-06-01

    Exit from the endoplasmic reticulum (ER) often occurs at distinct sites of vesicle formation known as transitional ER (tER) that are enriched for COPII vesicle coat proteins. We have characterized the organization of ER export in the malaria parasite, Plasmodium falciparum, by examining the localization of two components of the COPII machinery, PfSec12 and PfSec24a. PfSec12 was found throughout the ER, whereas the COPII cargo adaptor, PfSec24a, was concentrated at distinct foci that likely correspond to tER sites. These foci were closely apposed to cis-Golgi sites marked by PfGRASP-GFP, and upon treatment with brefeldin A they accumulated a model cargo protein via a process dependent on the presence of an intact diacidic export motif. Our data suggest that the cargo-binding function of PfSec24a is conserved and that accumulation of cargo in discrete tER sites depends upon positive sorting signals. Furthermore, the number and position of tER sites with respect to the cis-Golgi suggests a co-ordinated biogenesis of these domains.

  17. 49 CFR 1544.202 - Persons and property onboard an all-cargo aircraft.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Persons and property onboard an all-cargo aircraft. 1544.202 Section 1544.202 Transportation Other Regulations Relating to Transportation (Continued... SECURITY: AIR CARRIERS AND COMMERCIAL OPERATORS Operations § 1544.202 Persons and property onboard an...

  18. 49 CFR 1549.101 - Acceptance, screening, and transfer of cargo.

    Science.gov (United States)

    2010-10-01

    ... approved by TSA, an indirect air carrier under 49 CFR part 1548, an aircraft operator under part 1544, or a... onboard an aircraft of any unauthorized explosives, incendiaries, and other destructive substances or items in cargo onboard an aircraft, as provided in the facility's security program. (b) Screening...

  19. 77 FR 50926 - Security Zones; Certain Dangerous Cargo Vessels, Tampa, FL

    Science.gov (United States)

    2012-08-23

    ... DHS Department of Homeland Security CDC Certain Dangerous Cargo FR Federal Register NPRM Notice of... boarding and inspection of the vessel and persons onboard. The security zones would be enforced from 12:01... the vessel and persons onboard. (4) The Coast Guard will provide notice of the regulated areas...

  20. Endocytic Trafficking of Membrane-Bound Cargo: A Flotillin Point of View

    Directory of Open Access Journals (Sweden)

    Melanie Meister

    2014-07-01

    Full Text Available The ubiquitous and highly conserved flotillin proteins, flotillin-1 and flotillin-2, have been shown to be involved in various cellular processes such as cell adhesion, signal transduction through receptor tyrosine kinases as well as in cellular trafficking pathways. Due to the fact that flotillins are acylated and form hetero-oligomers, they constitutively associate with cholesterol-enriched lipid microdomains. In recent years, such microdomains have been appreciated as platforms that participate in endocytosis and other cellular trafficking steps. This review summarizes the current findings on the role of flotillins in membrane-bound cargo endocytosis and endosomal trafficking events. We will discuss the proposed function of flotillins in endocytosis in the light of recent findings that point towards a role for flotillins in a step that precedes the actual endocytic uptake of cargo molecules. Recent findings have also revealed that flotillins may be important for endosomal sorting and recycling of specific cargo molecules. In addition to these aspects, the cellular trafficking pathway of flotillins themselves as potential cargo in the context of growth factor signaling will be discussed.

  1. 75 FR 65437 - Defense Federal Acquisition Regulation Supplement; Defense Cargo Riding Gang Members (DFARS Case...

    Science.gov (United States)

    2010-10-25

    ... Acquisition Regulation Supplement; Defense Cargo Riding Gang Members (DFARS Case 2007-D002) AGENCY: Defense... 3504 addresses requirements that apply to riding gang members and DoD- exempted individuals who perform.... 109-364). Section 3504 addresses requirements that apply to riding gang members and...

  2. 15 CFR 30.26 - Reporting of vessels, aircraft, cargo vans, and other carriers and containers.

    Science.gov (United States)

    2010-01-01

    ... vehicles, trailers, pallets, cargo vans, lift vans, or similar shipping containers are not considered... transfer of ownership or title, in their capacity as carriers of goods or as instruments of such carriers... to sale or other transfer from ownership in the United States to ownership abroad. If a vessel,...

  3. The Arteries of Global Trade: Industrial Restructuring and Technological Change in the Transatlantic Air Cargo Industry

    Science.gov (United States)

    Schwarz, Guido

    2010-01-01

    Air cargo enjoys a special importance: together with maritime transport it is the backbone of global trade and is indispensable for contemporary globalization. Air transport is the only mode that combines worldwide reach with high speed. Nonetheless there is a dearth of geographic research that analyzes the current restructuring affecting the air…

  4. Dragonair- Year-on-Year Rises Continue For Passengers And Cargo

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Passenger numbers at Dragonair rose 13.2% while cargo volumes climbed 23.9%in April compared with the same month in 2004. The airline flew 431,860 passengers last month, 50,274 more than one year ago,and 7.3% up on March.

  5. 29 CFR 1917.22 - Hazardous cargo 2 (See § 1917.2(p)).

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Hazardous cargo 2 (See § 1917.2(p)). 1917.22 Section 1917.22 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... § 1917.2(p)). 2 The Department of Transportation and the United States Coast Guard apply...

  6. 46 CFR 308.549 - Application for appointment of Cargo Underwriting Agent, Form MA-319.

    Science.gov (United States)

    2010-10-01

    ..., Form MA-319. 308.549 Section 308.549 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... appointment of Cargo Underwriting Agent, Form MA-319. Any domestic insurance company authorized to do a marine... by submitting to the Maritime Administrator a letter and Form MA-399, which may be obtained from...

  7. Intracellular Delivery of Molecular Cargo Using Cell-Penetrating Peptides and the Combination Strategies

    Directory of Open Access Journals (Sweden)

    Hua Li

    2015-08-01

    Full Text Available Cell-penetrating peptides (CPPs can cross cellular membranes in a non-toxic fashion, improving the intracellular delivery of various molecular cargos such as nanoparticles, small molecules and plasmid DNA. Because CPPs provide a safe, efficient, and non-invasive mode of transport for various cargos into cells, they have been developed as vectors for the delivery of genetic and biologic products in recent years. Most common CPPs are positively charged peptides. While delivering negatively charged molecules (e.g., nucleic acids to target cells, the internalization efficiency of CPPs is reduced and inhibited because the cationic charges on the CPPs are neutralized through the covering of CPPs by cargos on the structure. Even under these circumstances, the CPPs can still be non-covalently complexed with the negatively charged molecules. To address this issue, combination strategies of CPPs with other typical carriers provide a promising and novel delivery system. This review summarizes the latest research work in using CPPs combined with molecular cargos including liposomes, polymers, cationic peptides, nanoparticles, adeno-associated virus (AAV and calcium for the delivery of genetic products, especially for small interfering RNA (siRNA. This combination strategy remedies the reduced internalization efficiency caused by neutralization.

  8. 19 CFR 123.92 - Electronic information for truck cargo required in advance of arrival.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Electronic information for truck cargo required in advance of arrival. 123.92 Section 123.92 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY CUSTOMS RELATIONS WITH CANADA AND MEXICO Advance...

  9. 19 CFR 122.49 - Correction of air cargo manifest or air waybill.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Correction of air cargo manifest or air waybill. 122.49 Section 122.49 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND...; Electronic Manifest Requirements for Passengers, Crew Members, and Non-Crew Members Onboard...

  10. 19 CFR 123.91 - Electronic information for rail cargo required in advance of arrival.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Electronic information for rail cargo required in advance of arrival. 123.91 Section 123.91 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY CUSTOMS RELATIONS WITH CANADA AND MEXICO Advance...

  11. 76 FR 5586 - California State Motor Vehicle and Nonroad Engine Pollution Control Standards; Mobile Cargo...

    Science.gov (United States)

    2011-02-01

    ... Equipment). CARB's Mobile Cargo Handling Equipment requirements are designed to use best available control... Alexander at (202) 343-9540, to learn if a hearing will be held or may check the following webpage for an... restricted by statute. EPA's Office of Transportation and Air Quality also maintains a webpage that...

  12. Supramolecular Controlled Cargo Release via Near Infrared Tunable Cucurbit[7]uril-Gold Nanostars.

    Science.gov (United States)

    Han, Yanwei; Yang, Xiran; Liu, Yingzhu; Ai, Qiushuang; Liu, Simin; Sun, Chunyan; Liang, Feng

    2016-02-26

    The near infrared (NIR) absorption and average particle size of gold nanostars (GNSs) can be precisely controlled by varying the molar ratios of cucurbit[7]urils (CB[7]) and GNSs in aqueous solution. GNSs modified with CB[7] achieved high cargo loading with thermally activated release upon the NIR laser irradiation.

  13. Functional description for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB)

    Energy Technology Data Exchange (ETDEWEB)

    Truett, L.F.; Rollow, J.P.; Shipe, P.C. [Oak Ridge National Lab., TN (United States); Faby, E.Z.; Fluker, J.; Hancock, W.R.; Grubb, J.W.; Russell, D.L. [Univ. of Tennessee, Knoxville, TN (United States); Ferguson, R.A. [SAIC, Oak Ridge, TN (United States)

    1995-12-15

    This Functional Description for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB) documents the purpose of and requirements for the ICDB in order to ensure a mutual understanding between the development group and the user group of the system. This Functional Description defines ICDB and provides a clear statement of the initial operational capability to be developed.

  14. 33 CFR 149.135 - What should be marked on the cargo transfer system alarm switch?

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false What should be marked on the cargo transfer system alarm switch? 149.135 Section 149.135 Navigation and Navigable Waters COAST GUARD... switch? Each switch for activating an alarm, and each audio or visual device for signaling an alarm,...

  15. Database specification for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB)

    Energy Technology Data Exchange (ETDEWEB)

    Faby, E.Z.; Fluker, J.; Hancock, B.R.; Grubb, J.W.; Russell, D.L. [Univ. of Tennessee, Knoxville, TN (United States); Loftis, J.P.; Shipe, P.C.; Truett, L.F. [Oak Ridge National Lab., TN (United States)

    1994-03-01

    This Database Specification for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB) describes the database organization and storage allocation, provides the detailed data model of the logical and physical designs, and provides information for the construction of parts of the database such as tables, data elements, and associated dictionaries and diagrams.

  16. 77 FR 6135 - Agency Information Collection Activities: Cargo Manifest/Declaration, Stow Plan, Container Status...

    Science.gov (United States)

    2012-02-07

    .../ Declaration, Stow Plan, Container Status Messages and Importer Security Filing AGENCY: U.S. Customs and Border... Manifest/Declaration, Stow Plan, Container Status Messages and Importer Security Filing. This is a proposed... forms of information. Title: Cargo Manifest/Declaration, Stow Plan, Container Status Messages...

  17. Scientific opinion on the evaluation of substances as acceptable previous cargoes for edible fats and oils

    DEFF Research Database (Denmark)

    Petersen, Annette

    , ethyl tert-butyl ether (ETBE) and ammonium sulphate. The evaluation was based on the same criteria as those used for the evaluation of the substances currently on the list in the Annex to Commission Directive 96/3/EC as acceptable previous cargoes for edible fats and oils. Methyl acetate and ETBE meet...

  18. 实货制条件下货运站装卸能力适应性研究%Study on Adaptability of Cargo Terminal Loading/unloading Capacity under Cargo Registration System

    Institute of Scientific and Technical Information of China (English)

    程堪弟; 殷勇

    2016-01-01

    随着“实货制”货运站装卸作业方式的转变,货运站如何实现白货的装卸、配载是个棘手问题,加上我国铁路现有的货运装卸方法存在一定的不适应性等问题,导致货运站装卸能力严重紧张不足。结合我国货物运输组织改革及主要货运站能力现状,确定影响货运站装卸效率主要因素,进一步提出改进实货制条件下货运站装卸能力适应性的保障措施。%The implementation of the cargo registration system has transformed the activity mode of the cargo terminals and moreover the loading/unloading process of the railway system in China is still misaligned with the cargo registration system, leading to severe shortage of the loading/unloading capacity of the cargo terminals. In this paper, in view of the reform initiative of the cargo transportation organizations in China and the current status of the capacity of the major cargo terminals, we determined the major factors affecting the loading/unloading efficiency of the cargo terminals and then further proposed the measures to improve the adaptability of the cargo terminals with the cargo registration system.

  19. Circular Chain Ropeway for Cargo Transportation in Mountain Citrus Orchard%山地橘园链式循环货运索道设计

    Institute of Scientific and Technical Information of China (English)

    洪添胜; 苏建; 朱余清; 杨洲; 岳学军; 宋淑然

    2011-01-01

    A circular chain cargo transportation ropeway system was proposed and developed for a simplified and labor-saving method to transport fruit and production materials in mountain citrus orchards. The ropeway system was composed of driving devices, hoisting chain, horizontal chain pulley, steering mechanism, automatic tensioning device, vertical chain pulley, combined chain pulley and cargo hooks. With the in-situ ropeway system installed in Guangdong and Jiangxi provinces, the chain circulation movement including up and down hill, steering and straight operations around the mountain was realized, fruit and production materials could be easily uploaded and downloaded by farmers standing on the ground, thereby the labor-saving and effective mountain citrus orchard cargo transportation was achieved.%为解决山地橘园的果品和农资等轻简化和省力化运送问题,提出了一种链式循环货运索道技术.该索道由驱动装置、起重链索、支架、水平托索机构、转向机构、自动张紧机构、垂直托索机构、组合托索机构和物品挂钩等组成.在广东省龙门县和江西省安远县的山地橘园实地安装此索道,链索实现了上下坡、转弯和直线的循环运行,果农站在地面可随意上载或下卸物品,最高生产率可达6.8t/h,实现了山地橘园物品省力和有效运送的目的.

  20. Impact of a radio-frequency identification system and information interchange on clearance processes for cargo at border posts

    Directory of Open Access Journals (Sweden)

    Ernest Bhero

    2015-02-01

    Full Text Available Background: Improved operational efficiency is important to role players in cross-border logistics and trade corridors. Cargo owners and cargo forwarders have been particularly concerned about long delays in the processing and clearing of cargo at border posts. Field studies suggest that these delays are due to a combination of factors, such as a lack of optimum system configurations and non-optimised human-dependent operations, which make the operations prone to corruption and other malpractices.Objectives: This article presents possible strategies for improving some of the operations in this sector. The research hinges on two key questions: (1 what is the impact of information interchange between stakeholders on the cargo transit time and (2 how will cargo transit time be impacted upon by automatic identification of cargo and the status of cargo seals on arriving vehicles at the border?Method: The use of information communication systems enabled by automatic identification systems (incorporating radio-frequency identification technology is suggested.Results: Results obtained by the described simulation model indicate that improvements of up to 82% with regard to transit time are possible using these techniques.Conclusion: The findings therefore demonstrate how operations at border posts can be improved through the use of appropriate technology and configuration of the operations.

  1. The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.

    Science.gov (United States)

    Topalidou, Irini; Cattin-Ortolá, Jérôme; Pappas, Andrea L; Cooper, Kirsten; Merrihew, Gennifer E; MacCoss, Michael J; Ailion, Michael

    2016-05-01

    The dense-core vesicle is a secretory organelle that mediates the regulated release of peptide hormones, growth factors, and biogenic amines. Dense-core vesicles originate from the trans-Golgi of neurons and neuroendocrine cells, but it is unclear how this specialized organelle is formed and acquires its specific cargos. To identify proteins that act in dense-core vesicle biogenesis, we performed a forward genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We previously reported the identification of two conserved proteins that interact with the small GTPase RAB-2 to control normal dense-core vesicle cargo-sorting. Here we identify several additional conserved factors important for dense-core vesicle cargo sorting: the WD40 domain protein EIPR-1 and the endosome-associated recycling protein (EARP) complex. By assaying behavior and the trafficking of dense-core vesicle cargos, we show that mutants that lack EIPR-1 or EARP have defects in dense-core vesicle cargo-sorting similar to those of mutants in the RAB-2 pathway. Genetic epistasis data indicate that RAB-2, EIPR-1 and EARP function in a common pathway. In addition, using a proteomic approach in rat insulinoma cells, we show that EIPR-1 physically interacts with the EARP complex. Our data suggest that EIPR-1 is a new interactor of the EARP complex and that dense-core vesicle cargo sorting depends on the EARP-dependent trafficking of cargo through an endosomal sorting compartment.

  2. Detection of Special Nuclear Material in Cargo Containers Using Neutron Interrogation

    Energy Technology Data Exchange (ETDEWEB)

    Slaughter, D; Accatino, M; Bernstein, A; Candy, J; Dougan, A; Hall, J; Loshak, A; Manatt, D; Meyer, A; Pohl, B; Prussin, S; Walling, R; Weirup, D

    2003-08-01

    The goal of the work reported here is to develop a concept for an active neutron interrogation system that can detect small targets of SNM contraband in cargo containers, roughly 5 kg HEU or 1 kg Pu, even when well shielded by a thick cargo. It is essential that the concept be reliable and have low false-positive and false-negative error rates. It also must be rapid to avoid interruption of commerce, completing the analysis in minutes. A new radiation signature unique to SNM has been identified that utilizes high-energy (E{sub {gamma}} = 3-7 MeV) fission product {gamma}-ray emission. Fortunately, this high-energy {gamma}-ray signature is robust in that it is very distinct compared to normal background radiation where there is no comparable high-energy {gamma}-ray radiation. Equally important, it has a factor of 10 higher yield than delayed neutrons that are the basis of classical interrogation technique normally used on small unshielded specimens of SNM. And it readily penetrates two meters of low-Z and high-Z cargo at the expected density of {approx} 0.5 gm/cm{sup 3}. Consequently, we expect that in most cases the signature flux at the container wall is at least 2-3 decades more intense than delayed neutron signals used historically and facilitates the detection of SNM even when shielded by thick cargo. Experiments have verified this signature and its predicted characteristics. However, they revealed an important interference due to the activation of {sup 16}O by the {sup 16}O(n,p){sup 16}N reaction that produces a 6 MeV {gamma}-ray following a 7-sec {beta}-decay of the {sup 16}N. This interference is important when irradiating with 14 MeV neutrons but is eliminated when lower energy neutron sources are utilized since the reaction threshold for {sup 16}O(n,p){sup 16}N is 10 MeV. The signature {gamma}-ray fluxes exiting a thick cargo can be detected in large arrays of scintillation detectors to produce useful signal count rates of 2-4 x 10{sup 4} cps. That is high

  3. Evaluation difference between mass of received cargo and mass of handed over the cargo in the determination of the masses by draft survey

    Directory of Open Access Journals (Sweden)

    Yakuta I. V.

    2016-12-01

    Full Text Available The paper provides the analysis of problems associated with the evaluation of difference between the mass of received and handed over cargo in determining the masses by draft survey and due to the difference in the measurement conditions at the loading and unloading ports (due to the change errors in various stages of the measurement procedures. The errors that may arise in determining the mass of the cargo due to roughness when measuring draft, due to using the inclinometer to determine the draft from one of boards, due to instrumental errors in the determination of the density of seawater, due to other possible errors have been investigated and evaluated. To estimate the errors of draft due to heaving and errors of inclinometer some formula are to be applied, their derivation has been done in this paper. It has been recommended to use the traditional formula of high-speed drawdown with the replacement of vessel speed on current rate to calculate the error of precipitation arising from the drawdowns ship on a current. The value per unit displacement draft from loading scale has been used to evaluate the error of the displacement appearing in the presence of draft errors. As a result two similar criteria (rigorous and statistical of allowable discrepancies calculated by draft survey mass of cargo in the port of loading and port of discharge have been substantiated. These criteria require the calculation and accumulation in a table of all the errors and calculate the total error of displacement. Criteria will allow the consignee and the carrier come to a reasonable and agreed decision about the significance of differences of the masses taking into account the indifference of conditions and measuring instruments.

  4. MAPEAMENTO DAS COMPETÊNCIAS E DESCRIÇÃO DE CARGOS DA LIVRARIA NOBEL DE PIRACICABA.

    OpenAIRE

    Almeida, Andreza Fernanda de; Universidade Metodista de Piracicaba (UNIMEP), Brasil; Cremonezi, Graziela Oste Graziano; Universidade Metodista de Piracicaba (UNIMEP), Brasil; Rocha, Maria Josilene Fontinele; Faculdade de Ensino Superior da Amazônia Reunida (FESAR), Brasil; Aguiar, Verônica Marinho; Faculdade de Ensino Superior da Amazônia Reunida (FESAR), Brasil; Franco, Ana Cristina Souza; Faculdade de Ensino Superior da Amazônia Reunida (FESAR), Brasil

    2014-01-01

    Este trabalho tem por objetivo mapear as competências e desenvolver a descrição de cargos da livraria Nobel de Piracicaba. Tendo num primeiro momento descritas as teorias referenciais sobre competências, descrição de cargo e as competências técnicas e comportamentais necessárias para o exercício da função/cargo. A pesquisa foi caracterizada quanto à abordagem do problema como qualitativa; A técnica escolhida para coleta de dados baseou-se na observação direta da pesquisadora, bem como nas inf...

  5. A review of modeling issues and analysis methods for the thermal response of cargoes transported in the Safe Secure Trailer subjected to fire environments

    Energy Technology Data Exchange (ETDEWEB)

    Howell, J.R. [Univ. of Texas, Austin, TX (United States). Dept. of Mechanical Engineering; Larsen, M.E. [Sandia National Labs., Albuquerque, NM (United States). High Consequence Assessment and Technology Dept.

    1998-05-01

    This paper discusses thermal analysis in support of probabilistic risk assessment (PRA) to predict the heating of cargoes shipped in vehicles like the Safe Secure Trailer. Fire environments contribute very significantly to the risk associated with ground transport of special nuclear materials. The tradeoff between thermal model complexity and the affordable number of scenarios used to represent the hazard space is discussed as it impacts PRA. The relevant heat transfer mechanisms are discussed along with the applicability of methods from the literature for analysis of these mechanisms. Many of the subject`s real problems remain too complex for affordable and rigorous analysis. Available models are generally restricted to idealizations that are quickly obviated by real effects. Approximate treatment methods, striving to produce conservative, realistic estimates are also discussed.

  6. Design of Cargo Hold Ventilation for Bulk Carrier Carrying Dangerous Cargoes%散货船装载危险品货舱通风系统的设计

    Institute of Scientific and Technical Information of China (English)

    黄荣杰

    2014-01-01

    By analyzing the different requirements of the Rules for cargo hold ventilation of bulk carrier carrying all kinds of dangerous cargoes, this paper classiifes the types of cargo hold ventilation for carrying dangerous cargoes, describes the design and typical arrangement of each type of cargo hold ventilation including the relevant rules and standards in order to simplify the design of cargo hold ventilation and reduce the cost of shipbuilding under the premise of meeting ship’s speciifcation and the Rules' minimum requirement.%本文通过分析散货船危险品货物清单中各种货物的装载和运输对货舱通风的不同要求,对散货船装载危险品货舱通风的型式进行归类,简述每一类货舱通风的设计及典型布置,分析其中所涉及到的相关规范和标准,旨在满足船东技术规格书要求和规范最低配置的前提下尽可能简化货舱通风系统的设计,降低船舶的建造成本。

  7. Cargo and Container X-Ray Inspection with Intra-Pulse Multi-Energy Method for Material Discrimination

    Science.gov (United States)

    Saverskiy, Aleksandr Y.; Dinca, Dan-Cristian; Rommel, J. Martin

    The Intra-Pulse Multi-Energy (IPME) method of material discrimination mitigates main disadvantages of the traditional "interlaced" approach: ambiguity caused by sampling different regions of cargo and reduction of effective scanning speed. A novel concept of creating multi-energy probing pulses using a standing-wave structure allows maintaining a constant energy spectrum while changing the time duration of each sub-pulse and thus enables adaptive cargo inspection. Depending on the cargo density, the dose delivered to the inspected object is optimized for best material discrimination, maximum material penetration, or lowest dose to cargo. A model based on Monte-Carlo simulation and experimental reference points were developed for the optimization of inspection conditions.

  8. 75 FR 5846 - Deadline for Notification of Intent To Use the Airport Improvement Program (AIP) Primary, Cargo...

    Science.gov (United States)

    2010-02-04

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Deadline for Notification of Intent To Use the Airport Improvement Program (AIP) Primary, Cargo, and Nonprimary Entitlement Funds for Fiscal Year 2010 AGENCY: Federal...

  9. Influence of direct motor-motor interaction in models for cargo transport by a single team of motors

    CERN Document Server

    Bouzat, Sebastian; 10.1088/1478-3975/7/4/046009

    2010-01-01

    We analyze theoretically the effects of excluded-volume interactions between motors on the dynamics of a cargo driven by multiple motors. The model considered shares many commons with other recently proposed in the literature, with the addition of direct interaction between motors and motor back steps. The cargo is assumed to follow a continuum Langevin dynamics, while individual motors evolve following a Monte Carlo algorithm based on experimentally accessible probabilities for discrete forward and backward jumps, and attachment and detachment rates. The links between cargo and motors are considered as non linear springs. By means of numerical simulations we compute the relevant quantities characterizing the dynamical properties of the system, and we compare the results to those for non interacting motors. We find that interactions lead to quite relevant changes in the force-velocity relation for cargo, with a considerable reduction of the stall force, and cause also a notable decrease of the run length. The...

  10. 77 FR 19408 - Deadline for Notification of Intent To Use the Airport Improvement Program (AIP) Primary, Cargo...

    Science.gov (United States)

    2012-03-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Deadline for Notification of Intent To Use the Airport Improvement Program (AIP) Primary, Cargo, and Nonprimary Entitlement Funds for Fiscal Year 2012 AGENCY: Federal...

  11. 78 FR 20167 - Deadline for Notification of Intent To Use the Airport Improvement Program (AIP) Primary, Cargo...

    Science.gov (United States)

    2013-04-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Deadline for Notification of Intent To Use the Airport Improvement Program (AIP) Primary, Cargo, and Nonprimary Entitlement Funds for Fiscal Year 2013 AGENCY: Federal...

  12. Attenuation Characteristic Modeling of Power-line Channel in Cargo Ships

    Science.gov (United States)

    Tsuzuki, Shinji; Ishii, Seiichiro; Nishioka, Jun; Yamada, Yoshio

    Power-line communication (PLC) based local area network (LAN) in cargo ships is one of the promising applications of the PLC technology. In this paper, the signal attenuation characteristic when transmitting with the differential-mode (DIF) and the common-mode (COM) has been modeled respectively to design the PLC network in a cargo ship. The characteristic has been analyzed from the measured transfer function of 440V power-line channels for reefer containers in two typical ships. The measured transfer function was averaged from 2M to 30MHz (or until the measurement limit frequency) and approximated by the number of branches and the length of a signal propagating route. The mean accuracy of the obtained approximation equations was ±4dB. The area where the signal attenuation of COM transmission is less than that of DIF transmission has been studied from the equations.

  13. Model of Optimal Cargo Transport Structure by Full Container Ship on Predefined Sailing Route

    Directory of Open Access Journals (Sweden)

    Serđo Kos

    2004-01-01

    Full Text Available This paper presents the mathematical model for solving theproblem of defining optimal cargo transport structure, occurringwhen, on a predefined sailing route, adequate number ofcontainers of various types, masses and sizes, possibly includingRO!RO cargo, is to be selected, i.e., a "container lot" is to beestablished in loading ports with the aim of gaining maximumship profit and, at the same time, of exploiting useful load andtransport capacity of container ship as much as possible. Theimplementation of the proposed model enables considerableincrease in the efficiency of container ship operations. Themodel was tested using a numerical example with real data.The applied post-optimal analysis examines the influence ofchange in some values of the mathematical model on the resultingoptimal program.

  14. Magnetically triggered release of molecular cargo from iron oxide nanoparticle loaded microcapsules

    Science.gov (United States)

    Carregal-Romero, Susana; Guardia, Pablo; Yu, Xiang; Hartmann, Raimo; Pellegrino, Teresa; Parak, Wolfgang J.

    2014-12-01

    Photothermal release of cargo molecules has been extensively studied for bioapplications. For instance, microcapsules decorated with plasmonic nanoparticles have been widely used in in vitro assays. However, some concerns about their suitability for some in vivo applications cannot be easily overcome, in particular the limited penetration depth of light (even infrared). Magnetic nanoparticles are alternative heat-mediators for local heating, which can be triggered by applying an alternating magnetic field (AMF). AMFs are much less absorbed by tissue than light and thus can penetrate deeper overcoming the above mentioned limitations. Here we present iron oxide nanocube-modified microcapsules as a platform for magnetically triggered molecular release. Layer-by-layer assembled polyelectrolyte microcapsules with 4.6 μm diameter, which had 18 nm diameter iron oxide nanocubes integrated in their walls, were synthesized. The microcapsules were further loaded with an organic fluorescent polymer (Cascade Blue-labelled dextran), which was used as a model of molecular cargo. Through an AMF the magnetic nanoparticles were able to heat their surroundings and destroy the microcapsule walls, leading to a final release of the embedded cargo to the surrounding solution. The cargo release was monitored in solution by measuring the increase in both absorbance and fluorescence signal after the exposure to an AMF. Our results demonstrate that magnetothermal release of the encapsulated material is possible using magnetic nanoparticles with a high heating performance.Photothermal release of cargo molecules has been extensively studied for bioapplications. For instance, microcapsules decorated with plasmonic nanoparticles have been widely used in in vitro assays. However, some concerns about their suitability for some in vivo applications cannot be easily overcome, in particular the limited penetration depth of light (even infrared). Magnetic nanoparticles are alternative heat

  15. Technologies and technical tools of combined transportation of cargoes by factors of global logistics

    Directory of Open Access Journals (Sweden)

    Анатолій Миколайович Котенко

    2015-01-01

    Full Text Available The new piggybacks technology has been developed and presented taking into account the problems of global logistics. The technology allows cargo operations (loading and unloading of modules on platforms of varying floor height above rail head level and reload modules between platforms with different values and different ways between rails floor height platforms. The system of differential equations of Kolmogorov have been showed and modeling of combined transportation have been made

  16. Identification of Cargo for Adaptor Protein (AP) Complexes 3 and 4 by Sucrose Gradient Profiling.

    Science.gov (United States)

    Pertl-Obermeyer, Heidi; Wu, Xu Na; Schrodt, Jens; Müdsam, Christina; Obermeyer, Gerhard; Schulze, Waltraud X

    2016-09-01

    Intracellular vesicle trafficking is a fundamental process in eukaryotic cells. It enables cellular polarity and exchange of proteins between subcellular compartments such as the plasma membrane or the vacuole. Adaptor protein complexes participate in the vesicle formation by specific selection of the transported cargo. We investigated the role of the adaptor protein complex 3 (AP-3) and adaptor protein complex 4 (AP-4) in this selection process by screening for AP-3 and AP-4 dependent cargo proteins. Specific cargo proteins are expected to be mis-targeted in knock-out mutants of adaptor protein complex components. Thus, we screened for altered distribution profiles across a density gradient of membrane proteins in wild type versus ap-3β and ap-4β knock-out mutants. In ap-3β mutants, especially proteins with transport functions, such as aquaporins and plasma membrane ATPase, as well as vesicle trafficking proteins showed differential protein distribution profiles across the density gradient. In the ap-4β mutant aquaporins but also proteins from lipid metabolism were differentially distributed. These proteins also showed differential phosphorylation patterns in ap-3β and ap-4β compared with wild type. Other proteins, such as receptor kinases were depleted from the AP-3 mutant membrane system, possibly because of degradation after mis-targeting. In AP-4 mutants, membrane fractions were depleted for cytochrome P450 proteins, cell wall proteins and receptor kinases. Analysis of water transport capacity in wild type and mutant mesophyll cells confirmed aquaporins as cargo proteins of AP-3 and AP-4. The combination of organelle density gradients with proteome analysis turned out as a suitable experimental strategy for large-scale analyses of protein trafficking.

  17. United States Seaport Security: Protection Against a Nuclear Device Attack Delivered in a Shipping Cargo Container

    Science.gov (United States)

    2014-06-13

    phone, laptop, airline mechanic’s certificate, and security passes for airports in Egypt, Thailand , and Canada. The following year, in March 2002...major operation areas for federal crime, including drug trafficking , cargo theft, and smuggling of contraband and aliens. 3. Ports are open and...container’s latch and continuously collects and transmits data without human intervention. This procedure is concurrently utilized as part of the C-TPAT

  18. High Power Nuclear Electric Propulsion (NEP) for Cargo and Propellant Transfer Missions in Cislunar Space

    Science.gov (United States)

    Falck, Robert D.; Borowski, Stanley K.

    2003-01-01

    The performance of Nuclear Electric Propulsion (NEP) in transporting cargo and propellant from Low Earth Orbit (LEO) to the first Earth-Moon Lagrange point (EML1) is examined. The baseline NEP vehicle utilizes a fission reactor system with Brayton power conversion for electric power generation to power multiple liquid hydrogen magnetoplasmadynamic (MPD) thrusters. Vehicle characteristics and performance levels are based on technology availability in a fifteen to twenty year timeframe. Results of numerical trajectory analyses are also provided.

  19. 46 CFR 154.1860 - Integral tanks: Cargo colder than −10 °C (14 °F).

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Integral tanks: Cargo colder than â10 °C (14 °F). 154.1860 Section 154.1860 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK....1860 Integral tanks: Cargo colder than −10 °C (14 °F). The master shall ensure that an integral...

  20. An Optimization Model of the Single-Leg Air Cargo Space Control Based on Markov Decision Process

    Directory of Open Access Journals (Sweden)

    Chun-rong Qin

    2012-01-01

    Full Text Available Based on the single-leg air cargo issues, we establish a dynamic programming model to consider the overbooking and space inventory control problem. We analyze the structure of optimal booking policy for every kind of booking requests and show that the optimal booking decision is of threshold type (known as booking limit policy. Our research provides a theoretical support for the air cargo space control.

  1. TRE17/USP6 regulates ubiquitylation and trafficking of cargo proteins that enter cells by clathrin-independent endocytosis

    OpenAIRE

    Funakoshi, Yuji; Chou, Margaret M.; Kanaho, Yasunori; Julie G Donaldson

    2014-01-01

    Plasma membrane proteins that enter cells by clathrin-independent endocytosis (CIE) are sorted either to lysosomes for degradation or recycled back to the plasma membrane. Expression of some MARCH E3 ubiquitin ligases promotes trafficking of CIE cargo proteins to lysosomes by ubiquitylating the proteins. Here, we show that co-expression of the ubiquitin-specific protease TRE17/USP6 counteracts the MARCH-dependent targeting of CIE cargo proteins, but not that of transferrin receptor, to lysoso...

  2. Research on characteristics of radiated noise of large cargo ship in shallow water

    Science.gov (United States)

    Liu, Yongdong; Zhang, Liang

    2017-01-01

    With the rapid development of the shipping industry, the number of the world's ship is gradually increasing. The characteristics of the radiated noise of the ship are also of concern. Since the noise source characteristics of multichannel interference, the surface wave and the sea temperature microstructure and other reasons, the sound signal received in the time-frequency domain has varying characteristics. The signal of the radiated noise of the large cargo ship JOCHOH from horizontal hydrophone array in some shallow water of China is processed and analyzed in the summer of 2015, and the results show that a large cargo ship JOCHOH has a number of noise sources in the direction of the ship's bow and stern lines, such as host, auxiliary and propellers. The radiating sound waves generated by these sources do not meet the spherical wave law at lower frequency in the ocean, and its radiated noise has inherent spatial distribution, the variation characteristics of the radiated noise the large cargo ship in time and frequency domain are given. The research method and results are of particular importance.

  3. Reliability analysis of the bulk cargo loading system including dependent components

    Science.gov (United States)

    Blokus-Roszkowska, Agnieszka

    2016-06-01

    In the paper an innovative approach to the reliability analysis of multistate series-parallel systems assuming their components' dependency is presented. The reliability function of a multistate series system with components dependent according to the local load sharing rule is determined. Linking these results for series systems with results for parallel systems with independent components, we obtain the reliability function of a multistate series-parallel system assuming dependence of components' departures from the reliability states subsets in series subsystem and assuming independence between these subsystems. As a particular case, the reliability function of a multistate series-parallel system composed of dependent components having exponential reliability functions is fixed. Theoretical results are applied practically to the reliability evaluation of a bulk cargo transportation system, which main area is to load bulk cargo on board the ships. The reliability function and other reliability characteristics of the loading system are determined in case its components have exponential reliability functions with interdependent departures rates from the subsets of their reliability states. Finally, the obtained results are compared with results for the bulk cargo transportation system composed of independent components.

  4. Structural Design and Analysis of Un-pressurized Cargo Delivery Vehicle

    Science.gov (United States)

    Martinovic, Zoran N.

    2007-01-01

    As part of the Exploration Systems Architecture Study, NASA has defined a family of vehicles to support lunar exploration and International Space Station (ISS) re-supply missions after the Shuttle s retirement. The Un-pressurized Cargo Delivery Vehicle (UCDV) has been envisioned to be an expendable logistics delivery vehicle that would be used to deliver external cargo to the ISS. It would be launched on the Crew Launch Vehicle and would replace the Crew Exploration Vehicle. The estimated cargo would be the weight of external logistics to the ISS. Determining the minimum weight design of the UCDV during conceptual design is the major issue addressed in this paper. This task was accomplished using a procedure for rapid weight estimation that was based on Finite Element Analysis and sizing of the vehicle by the use of commercially available codes. Three design concepts were analyzed and their respective weights were compared. The analytical structural weight was increased by a factor to account for structural elements that were not modeled. Significant reduction in weight of a composite design over metallic was achieved for similar panel concepts.

  5. Importance of anisotropy in detachment rates for force production and cargo transport by a team of motor proteins.

    Science.gov (United States)

    Takshak, Anjneya; Kunwar, Ambarish

    2016-05-01

    Many cellular processes are driven by collective forces generated by a team consisting of multiple molecular motor proteins. One aspect that has received less attention is the detachment rate of molecular motors under mechanical force/load. While detachment rate of kinesin motors measured under backward force increases rapidly for forces beyond stall-force; this scenario is just reversed for non-yeast dynein motors where detachment rate from microtubule decreases, exhibiting a catch-bond type behavior. It has been shown recently that yeast dynein responds anisotropically to applied load, i.e. detachment rates are different under forward and backward pulling. Here, we use computational modeling to show that these anisotropic detachment rates might help yeast dynein motors to improve their collective force generation in the absence of catch-bond behavior. We further show that the travel distance of cargos would be longer if detachment rates are anisotropic. Our results suggest that anisotropic detachment rates could be an alternative strategy for motors to improve the transport properties and force production by the team.

  6. The intracellular cargo receptor ERGIC-53 is required for the production of infectious arenavirus, coronavirus, and filovirus particles.

    Science.gov (United States)

    Klaus, Joseph P; Eisenhauer, Philip; Russo, Joanne; Mason, Anne B; Do, Danh; King, Benjamin; Taatjes, Douglas; Cornillez-Ty, Cromwell; Boyson, Jonathan E; Thali, Markus; Zheng, Chunlei; Liao, Lujian; Yates, John R; Zhang, Bin; Ballif, Bryan A; Botten, Jason W

    2013-11-13

    Arenaviruses and hantaviruses cause severe human disease. Little is known regarding host proteins required for their propagation. We identified human proteins that interact with the glycoproteins (GPs) of a prototypic arenavirus and hantavirus and show that the lectin endoplasmic reticulum (ER)-Golgi intermediate compartment 53 kDa protein (ERGIC-53), a cargo receptor required for glycoprotein trafficking within the early exocytic pathway, associates with arenavirus, hantavirus, coronavirus, orthomyxovirus, and filovirus GPs. ERGIC-53 binds to arenavirus GPs through a lectin-independent mechanism, traffics to arenavirus budding sites, and is incorporated into virions. ERGIC-53 is required for arenavirus, coronavirus, and filovirus propagation; in its absence, GP-containing virus particles form but are noninfectious, due in part to their inability to attach to host cells. Thus, we have identified a class of pathogen-derived ERGIC-53 ligands, a lectin-independent basis for their association with ERGIC-53, and a role for ERGIC-53 in the propagation of several highly pathogenic RNA virus families.

  7. Smart Nanostructures for Cargo Delivery: Uncaging and Activating by Light.

    Science.gov (United States)

    Karimi, Mahdi; Sahandi Zangabad, Parham; Baghaee-Ravari, Soodeh; Ghazadeh, Mehdi; Mirshekari, Hamid; Hamblin, Michael R

    2017-03-13

    Nanotechnology has begun to play a remarkable role in various fields of science and technology. In biomedical applications, nanoparticles have opened new horizons, especially for biosensing, targeted delivery of therapeutics, and so forth. Among drug delivery systems (DDSs), smart nanocarriers that respond to specific stimuli in their environment represent a growing field. Nanoplatforms that can be activated by an external application of light can be used for a wide variety of photoactivated therapies, especially light-triggered DDSs, relying on photoisomerization, photo-cross-linking/un-cross-linking, photoreduction, and so forth. In addition, light activation has potential in photodynamic therapy, photothermal therapy, radiotherapy, protected delivery of bioactive moieties, anticancer drug delivery systems, and theranostics (i.e., real-time monitoring and tracking combined with a therapeutic action to different diseases sites and organs). Combinations of these approaches can lead to enhanced and synergistic therapies, employing light as a trigger or for activation. Nonlinear light absorption mechanisms such as two-photon absorption and photon upconversion have been employed in the design of light-responsive DDSs. The integration of a light stimulus into dual/multiresponsive nanocarriers can provide spatiotemporal controlled delivery and release of therapeutic agents, targeted and controlled nanosystems, combined delivery of two or more agents, their on-demand release under specific conditions, and so forth. Overall, light-activated nanomedicines and DDSs are expected to provide more effective therapies against serious diseases such as cancers, inflammation, infections, and cardiovascular disease with reduced side effects and will open new doors toward the treatment of patients worldwide.

  8. Cargo and Carrier Effects of Rapamycin-Loaded Perfluorocarbon Nanoparticles

    Science.gov (United States)

    Bibee, Kristin Page

    Nanoparticle-based drug delivery has been championed as a means to increase local delivery of therapeutics while decreasing systemic drug exposure. By targeting the particles, and therefore the drugs, to diseased cells of interest, healthy cells will be spared and side effects avoided. This delivery mechanism would be particularly useful for drugs that interfere with cell growth and proliferation pathways, as blocking proliferation in normal cells leads to significant patient morbidity. Rapamycin is a macrolide and a known inhibitor of mTORC1, a protein complex that plays a crucial role in protein translation and cell growth. This work demonstrates the effects of rapamycin complexed with a nanoparticle carrier on two distinct pathologies: a new triple negative breast cancer cell line and a conventional mouse model of muscular dystrophy (mdx). Rapamycin is able to alter mitochondrial function and thus metabolism in both free and nanoparticle-delivered form without killing the cells. Although nanoparticles are considered to be a benign carrier, this work shows that perfluorocarbon nanoparticles are able to induce autophagy in vitro. The benefits of autophagy induction in cancer cells is cell and stage specific, but has been reported to be useful for radiosensitization of triple negative breast cancers. Additionally, the particles are shown to induce autophagy in the mdx model of Duchenne Muscular Dystrophy and, when loaded with rapamycin, dramatically improve strength even in older animals with muscular dystrophy. Overall, this work enhances our understanding of the cellular effects of perfluorocarbon nanoparticles in two different disease models and enhances prospects for clinical translation of nanoparticle-based drug delivery.

  9. Design and model selection for unit cargo handling system at air cargo terminal%航空货运站集装货处理系统设计选型研究

    Institute of Scientific and Technical Information of China (English)

    梅宝兴

    2012-01-01

    航空货运站是专门处理航空货物的地面设施.根据近年来国内航空货运发展的新趋势,分析了集装货处理系统设计选型阶段需重点考虑的问题,并对存储容量、堆垛机数量等关键参数的确定进行了较为深入的研究,为航空货运集装货处理系统的设计优化提供了思路.%The air cargo terminal is the ground-level facility to specifically handle air cargoes. Based on the new trend of domestic air cargo market in recent years, certain key necessary problems jegarding design and model selection of unit cargo handling system are analyzed, with further research on determination of key parameters such as storage capacity and quantity of stackers, etc. , which provides ideas for design optimization of unit cargo handling system.

  10. Opposing microtubule motors control motility, morphology and cargo segregation during ER-to-Golgi transport

    Directory of Open Access Journals (Sweden)

    Anna K. Brown

    2014-04-01

    Full Text Available We recently demonstrated that dynein and kinesin motors drive multiple aspects of endosomal function in mammalian cells. These functions include driving motility, maintaining morphology (notably through providing longitudinal tension to support vesicle fission, and driving cargo sorting. Microtubule motors drive bidirectional motility during traffic between the endoplasmic reticulum (ER and Golgi. Here, we have examined the role of microtubule motors in transport carrier motility, morphology, and domain organization during ER-to-Golgi transport. We show that, consistent with our findings for endosomal dynamics, microtubule motor function during ER-to-Golgi transport of secretory cargo is required for motility, morphology, and cargo sorting within vesicular tubular carriers en route to the Golgi. Our data are consistent with previous findings that defined roles for dynein-1, kinesin-1 (KIF5B and kinesin-2 in this trafficking step. Our high resolution tracking data identify some intriguing aspects. Depletion of kinesin-1 reduces the number of motile structures seen, which is in line with other findings relating to the role of kinesin-1 in ER export. However, those transport carriers that were produced had a much greater run length suggesting that this motor can act as a brake on anterograde motility. Kinesin-2 depletion did not significantly reduce the number of motile transport carriers but did cause a similar increase in run length. These data suggest that kinesins act as negative regulators of ER-to-Golgi transport. Depletion of dynein not only reduced the number of motile carriers formed but also caused tubulation of carriers similar to that seen for sorting nexin-coated early endosomes. Our data indicated that the previously observed anterograde–retrograde polarity of transport carriers in transit to the Golgi from the ER is maintained by microtubule motor function.

  11. El acceso a los cargos de gobierno de la audiencia de Quito (1701-1750

    Directory of Open Access Journals (Sweden)

    Sanz Tapia, Ángel

    2006-12-01

    Full Text Available Study oft the designations of the posts of government (“presidencias”, “gobiernos”, ”alcaldías Mayores” y “corregimientos” in the “Audiencia” of Quito and its connection with the way to get them (by a service given, by a merit, by money…. The appointments were effected from Madrid during the first half of the 18th century. Analysis in detail of the posts and of the amounts of money that were paid as a benefit in order to get the post as well as some references to the same circumstances in the High Court at the end of the rign of Carlos II (1674-1700. Location of the designated personages, information about the dedication to their work and about their social standing. In conclusion: Two in three offices were got by money; the number of creoles in the Spanish - American governments increased in relation to the previous stage.

    Estudio de las provisiones de cargos de gobierno (presidencias audienciales, gobernaciones, alcaldías mayores y corregimientos de la audiencia de Quito efectuadas desde Madrid en relación con su modo de acceso (por méritos, por dinero, etc., durante la primera mitad del siglo XVIII. Análisis detallado de los cargos, cuantías abonadas por concepto de “beneficio”, y referencias a las mismas circunstancias de la audiencia en la etapa final de Carlos II (1674-1700. Localización de los personajes provistos y referencias sobre dedicación y categoría social. Conclusiones: 2 de cada 3 oficios se consiguieron mediante donativo pecuniario; hay un aumento del número de criollos en los cargos de gobierno indianos analizados en relación con la etapa precedente.

  12. Evidence of nose-to-brain delivery of nanoemulsions: cargoes but not vehicles.

    Science.gov (United States)

    Ahmad, Ejaj; Feng, Yunhai; Qi, Jianping; Fan, Wufa; Ma, Yuhua; He, Haisheng; Xia, Fei; Dong, Xiaochun; Zhao, Weili; Lu, Yi; Wu, Wei

    2017-01-19

    The nose-to-brain pathway has been proven to be a shortcut for direct drug delivery to the brain. However, whether and to what extent nanoparticles can be delivered through this passage is still awaiting validation with evidence. In this study, nose-to-brain transportation of nanoparticles is tracked via fluorescence bioimaging strategies using nanoemulsions (NEs) as model carriers. Identification of NEs in biological tissues is based on the on → off signal switching of a new type of environment-responsive embedded dyes, P2 and P4, and two conventional probes, DiR and coumarin-6 (C6), are embedded to represent the cargoes. Evidence for the translocation of NEs was collected either via live imaging or ex vivo histological examination in rats after nasal administration. Results suggest that NEs with a particle size of about 100 nm, either naked or coated with chitosan, have longer retention duration in nostrils and slower mucociliary clearance than larger ones. P2 signals, representing integral NEs, can be found in mucosa and trigeminal nerves for all size groups, whereas only weak P2 signals are detected in the olfactory bulb for chitosan-coated NEs of 100 nm. Confocal microscopy further confirms the translocation of integral 100 nm NEs in nasal mucosa and along the trigeminal nerve in decremental intensity. Weak signals of the P4 probe, also representing integral NEs, can be detected in the olfactory bulb but few in the brain. NEs as large as 900 nm cannot be transported to the olfactory bulb. However, the DiR or C6 signals that represent the cargoes can be found in significant amounts along the nose-to-brain pathway and finally reach the brain. Evidence shows that integral NEs can be delivered to the olfactory bulb, but few to the brain, whereas the cargoes can be released and permeated into the brain in greater amounts.

  13. Combining Radiography and Passive Measurements for Radiological Threat Localization in Cargo

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Erin A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); White, Timothy A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Jarman, Kenneth D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Kouzes, Richard T. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Kulisek, Jonathan A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Robinson, Sean M. [Pacific Northwest National Lab. (PNNL), Seattle, WA (United States); Wittman, Richard A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-10-01

    Detecting shielded special nuclear material (SNM) in a cargo container is a difficult problem, since shielding reduces the amount of radiation escaping the container. Radiography provides information that is complementary to that provided by passive gamma-ray detection systems: while not directly sensitive to radiological materials, radiography can reveal highly shielded regions that may mask a passive radiological signal. Combining these measurements has the potential to improve SNM detection, either through improved sensitivity or by providing a solution to the inverse problem to estimate source properties (strength and location). We present a data-fusion method that uses a radiograph to provide an estimate of the radiation-transport environment for gamma rays from potential sources. This approach makes quantitative use of radiographic images without relying on image interpretation, and results in a probabilistic description of likely source locations and strengths. We present results for this method for a modeled test case of a cargo container passing through a plastic-scintillator-based radiation portal monitor and a transmission-radiography system. We find that a radiograph-based inversion scheme allows for localization of a low-noise source placed randomly within the test container to within 40 cm, compared to 70 cm for triangulation alone, while strength estimation accuracy is improved by a factor of six. Improvements are seen in regions of both high and low shielding, but are most pronounced in highly shielded regions. The approach proposed here combines transmission and emission data in a manner that has not been explored in the cargo-screening literature, advancing the ability to accurately describe a hidden source based on currently-available instrumentation.

  14. Loss of cargo binding in the human myosin VI deafness mutant (R1166X) leads to increased actin filament binding.

    Science.gov (United States)

    Arden, Susan D; Tumbarello, David A; Butt, Tariq; Kendrick-Jones, John; Buss, Folma

    2016-10-01

    Mutations in myosin VI have been associated with autosomal-recessive (DFNB37) and autosomal-dominant (DFNA22) deafness in humans. Here, we characterise an myosin VI nonsense mutation (R1166X) that was identified in a family with hereditary hearing loss in Pakistan. This mutation leads to the deletion of the C-terminal 120 amino acids of the myosin VI cargo-binding domain, which includes the WWY-binding motif for the adaptor proteins LMTK2, Tom1 as well as Dab2. Interestingly, compromising myosin VI vesicle-binding ability by expressing myosin VI with the R1166X mutation or with single point mutations in the adaptor-binding sites leads to increased F-actin binding of this myosin in vitro and in vivo As our results highlight the importance of cargo attachment for regulating actin binding to the motor domain, we perform a detailed characterisation of adaptor protein binding and identify single amino acids within myosin VI required for binding to cargo adaptors. We not only show that the adaptor proteins can directly interact with the cargo-binding tail of myosin VI, but our in vitro studies also suggest that multiple adaptor proteins can bind simultaneously to non-overlapping sites in the myosin VI tail. In conclusion, our characterisation of the human myosin VI deafness mutant (R1166X) suggests that defects in cargo binding may leave myosin VI in a primed/activated state with an increased actin-binding ability.

  15. Comunidad, sistema de cargos y proyecto social. Una propuesta analítica de sociedades locales en México

    Directory of Open Access Journals (Sweden)

    Íñigo González de la Fuente

    2011-01-01

    Full Text Available El presente texto contiene una propuesta de análisis antropológico del sistema de cargos que incluye tanto a comunidades indígenas como no-indígenas. Partiendo del reconocimiento del sistema de cargos como una institución esencialmente heterogénea, se sugiere que, como hipótesis principal, tal institución alimenta una serie de mecanismos que, en sí mismos, potencian los escenarios de interacciones que protagonizan los miembros de la comunidad a través de un conjunto de roles: fomentan que los escenarios puedan ser protagonizados por el mayor número de miembros; propician que tales escenarios se articulen con la mayor frecuencia posible; todo ello con el acuerdo tácito entre protagonistas de que exista rotación de roles entre escenario y escenario. Esta propuesta analítica va a estar estructurada en tres bloques. En primer lugar, se ubican los fenómenos estudiados en torno al sistema de cargos en un continuum de comportamientos tendentes al individualismo y a lo colectivo en sus dos polos. En segundo lugar, se explicita el concepto de comunidad como elemento básico para el estudio de los sistemas de cargos; por último, se propone la idea del proyecto comunitario como denominador común en cuanto a sistemas de cargos se refiere en el área mesoamericana.

  16. Waste Crate and Container Imaging Using the Vehicle and Cargo Inspection System. Innovative Technology Summary Report

    Energy Technology Data Exchange (ETDEWEB)

    None

    2000-07-01

    The Vehicle and Cargo Inspection System (VACIS) is a highly penetrating gamma ray imaging system that provides a means to non-invasively image crate contents prior to crate disassembly. The VACIS unit uses a 1.6 Curie collimated source (Cesium-137) aimed at a linear detector to create an image as the unit passes by the crate. In the demonstrated mobile unit, the source and detector were mounted on a boom truck. As the crate passed between the source and detector, a near real-time composite image of the contents was constructed from the linear image of the VACIS unit's on board computer and recorded on disk.

  17. Progress cargo spacecraft observed with the AZT-33IK optical telescope

    Science.gov (United States)

    Klunko, Evgeniy; Eselevich, Maksim; Tergoev, Vladimir

    2016-09-01

    In this paper, we describe a telescope and measuring equipment used for optical observations of Progress cargo spacecraft (PCS), which were made during Radar-Progress space experiment sessions. We also describe object tracking and measurement techniques. The observations were made with the optical telescope AZT-33IK at Sayan Solar Observatory of ISTP SB RAS. During many of the sessions, we registered optical phenomena that occurred in regions of space surrounding PCS and appeared due to the work of PCS onboard engines. The data we obtained can be used to independently control the geometry of the experiment and to analyze physical conditions in outer space.

  18. SpaceX launches cargo ship but rocket recovery test ends in crash

    Institute of Scientific and Technical Information of China (English)

    Jonathan; Amos

    2015-01-01

    The American SpaceX firm says its experiment to bring part of its Falcon rocket down to a soft landing on a floating sea platform did not work.The vehicle was launched on a mission to send a cargo capsule to the International Space Station.But once the first-stage of the rocket completed its part of this task,it tried to make a controlled return.The company CEO Elon Musk tweeted that the booster had hit the platform hard.

  19. Mission Design Considerations for Mars Cargo of the Human Spaceflight Architecture Team's Evolvable Mars Campaign

    Science.gov (United States)

    Sjauw, Waldy K.; McGuire, Melissa L.; Freeh, Joshua E.

    2016-01-01

    Recent NASA interest in human missions to Mars has led to an Evolvable Mars Campaign by the agency's Human Architecture Team. Delivering the crew return propulsion stages and Mars surface landers, SEP based systems are employed because of their high specific impulse characteristics enabling missions requiring less propellant although with longer transfer times. The Earth departure trajectories start from an SLS launch vehicle delivery orbit and are spiral shaped because of the low SEP thrust. Previous studies have led to interest in assessing the divide in trip time between the Earth departure and interplanetary legs of the mission for a representative SEP cargo vehicle.

  20. Nuclear Car Wash sensitivity in varying thicknesses of wood and steel cargo

    Energy Technology Data Exchange (ETDEWEB)

    Church, J; Slaughter, D; Asztalos, S; Biltoft, P; Descalle, M; Hall, J; Manatt, D; Mauger, J; Norman, E; Petersen, D; Prussin, S

    2006-10-05

    The influence of incident neutron attenuation on signal strengths in the Nuclear Car Wash has been observed experimentally for both wood and steel-pipe mock cargos. Measured decay curves are presented for {beta}-delayed high-energy {gamma}-rays and thermalized neutrons following neutron-induced fission of HEU through varying irradiation lengths. Error rates are extracted for delayed-{gamma} and delayed-n signals integrated to 30 seconds, assuming Gaussian distributions for the active background. The extrapolation to a field system of 1 mA deuterium current and to a 5 kg sample size is discussed.

  1. Unruly Cargo.

    Science.gov (United States)

    Vail, Kathleen

    1997-01-01

    Incidents of unruliness, defiance, and even violence on the school bus are escalating. Many school districts find that a behavior-management focus in their bus-driver training pays off in fewer serious discipline incidents and more job satisfaction among drivers. (MLF)

  2. Transport properties of melanosomes along microtubules interpreted by a tug-of-war model with loose mechanical coupling.

    Directory of Open Access Journals (Sweden)

    Sebastián Bouzat

    Full Text Available In this work, we explored theoretically the transport of organelles driven along microtubules by molecular motors of opposed polarities using a stochastic model that considers a Langevin dynamics for the cargo, independent cargo-motor linkers and stepping motion for the motors. It has been recently proposed that the stiffness of the motor plays an important role when multiple motors collectively transport a cargo. Therefore, we considered in our model the recently reported values for the stiffness of the cargo-motor linker determined in living cells (∼0.01 pN/nm, which is significantly lower than the motor stiffness obtained in in vitro assays and used in previous studies. Our model could reproduce the multimodal velocity distributions and typical trajectory characteristics including the properties of the reversions in the overall direction of motion observed during melanosome transport along microtubules in Xenopus laevis melanophores. Moreover, we explored the contribution of the different motility states of the cargo-motor system to the different modes of the velocity distributions and could identify the microscopic mechanisms of transport leading to trajectories compatible with those observed in living cells. Finally, by changing the attachment and detachment rates, the model could reproduce the different velocity distributions observed during melanosome transport along microtubules in Xenopus laevis melanophores stimulated for aggregation and dispersion. Our analysis suggests that active tug-of-war processes with loose mechanical coupling can account for several aspects of cargo transport along microtubules in living cells.

  3. Anomalous transport of subdiffusing cargos by single kinesin motors: the role of mechanochemical coupling and anharmonicity of tether

    CERN Document Server

    Goychuk, Igor

    2014-01-01

    Here we generalize our previous model of molecular motors trafficking subdiffusing cargos in viscoelastic cytosol by (i) including mechanochemical coupling between cyclic conformational fluctuations of the motor protein driven by the reaction of ATP hydrolysis and its translational motion within the simplest two-state model of hand-over-hand motion of kinesin, and also (ii) by taking into account the anharmonicity of the tether between the motor and cargo (its maximally possible extension length). It is shown that the major earlier results such as occurrence of normal versus anomalous transport depending on the amplitude of binding potential, cargo size and the motor turnover frequency not only survive in this more realistic model, but the results also look very similar for the correspondingly adjusted parameters. However, this more realistic model displays a substantially larger thermodynamic efficiency due to a bidirectional mechanochemical coupling. For realistic parameters, the maximal thermodynamic effic...

  4. Microtubule-binding protein doublecortin-like kinase 1 (DCLK1) guides kinesin-3-mediated cargo transport to dendrites.

    Science.gov (United States)

    Lipka, Joanna; Kapitein, Lukas C; Jaworski, Jacek; Hoogenraad, Casper C

    2016-02-01

    In neurons, the polarized distribution of vesicles and other cellular materials is established through molecular motors that steer selective transport between axons and dendrites. It is currently unclear whether interactions between kinesin motors and microtubule-binding proteins can steer polarized transport. By screening all 45 kinesin family members, we systematically addressed which kinesin motors can translocate cargo in living cells and drive polarized transport in hippocampal neurons. While the majority of kinesin motors transport cargo selectively into axons, we identified five members of the kinesin-3 (KIF1) and kinesin-4 (KIF21) subfamily that can also target dendrites. We found that microtubule-binding protein doublecortin-like kinase 1 (DCLK1) labels a subset of dendritic microtubules and is required for KIF1-dependent dense-core vesicles (DCVs) trafficking into dendrites and dendrite development. Our study demonstrates that microtubule-binding proteins can provide local signals for specific kinesin motors to drive polarized cargo transport.

  5. Monte-Carlo Simulations of Radiation-Induced Activation in a Fast-Neutron and Gamma- Based Cargo Inspection System

    CERN Document Server

    Bromberger, B; Brandis, M; Dangendorf, V; Goldberg, M B; Kaufmann, F; Mor, I; Nolte, R; Schmiedel, M; Tittelmeier, K; Vartsky, D; Wershofen, H

    2012-01-01

    An air cargo inspection system combining two nuclear reaction based techniques, namely Fast-Neutron Resonance Radiography and Dual-Discrete-Energy Gamma Radiography is currently being developed. This system is expected to allow detection of standard and improvised explosives as well as special nuclear materials. An important aspect for the applicability of nuclear techniques in an airport inspection facility is the inventory and lifetimes of radioactive isotopes produced by the neutron and gamma radiation inside the cargo, as well as the dose delivered by these isotopes to people in contact with the cargo during and following the interrogation procedure. Using MCNPX and CINDER90 we have calculated the activation levels for several typical inspection scenarios. One example is the activation of various metal samples embedded in a cotton-filled container. To validate the simulation results, a benchmark experiment was performed, in which metal samples were activated by fast-neutrons in a water-filled glass jar. T...

  6. Sulina and Danube-Black Sea Channels: Competitors or Allies on Cargo Transport in South-Eastern Europe?

    Directory of Open Access Journals (Sweden)

    Ionica Soare

    2015-10-01

    Full Text Available The two river channels which connect the Black Sea to the inland Romanian area, have constituted since the beginning of their building two very important transport routes for the cargo and passenger traffic (Sulina that have connected Black Sea to the internal ports of Romania, thus representing ”transport highways” of the Romanian economy. The present study tries to present the chronological evolution of these two shipping routes, regarding their importance to the cargo traffic, as well as the financial policy imposed by the administration of these channels. We will present and analyze retrospectively the major elements regarding the administration of these channels, a comparative analysis of the role of these two channels in the river cargo transport, consequently trying to predict a future evolution of these two river communication routes.

  7. La confiabilidad en los sistemas eléctricos competitivos y el modelo colombiano de cargo por confiabilidad

    Directory of Open Access Journals (Sweden)

    Vélez Luis Guillermo

    2012-09-01

    Full Text Available El propósito de este artículo es hacer un análisis de la seguridad en el suministro de electricidad en Colombia, considerando el nuevo esquema del Cargo por Confiabilidad, modelo adoptado en Colombia para garantizar las inversiones en generación. Para esto se analiza la diferencia entre escenarios de demanda y de oferta de electricidad. La oferta para las plantas nuevas considera las OEF adquiridas con el cargo; y para las plantas existentes se realizan simulaciones bajo diversos escenarios de ocurrencia del fenómeno de “El Niño”. La demanda fue tomada de las proyecciones de la UPME. Las simulaciones realizadas muestran una apropiada dimensión del tamaño del Cargo por Confiabilidad.

  8. Engineering advanced capsosomes: maximizing the number of subcompartments, cargo retention, and temperature-triggered reaction.

    Science.gov (United States)

    Chandrawati, Rona; Hosta-Rigau, Leticia; Vanderstraaten, Dirk; Lokuliyana, Shalitha A; Städler, Brigitte; Albericio, Fernando; Caruso, Frank

    2010-03-23

    Advanced mimics of cells require a large yet controllable number of subcompartments encapsulated within a scaffold, equipped with a trigger to initiate, terminate, and potentially restart an enzymatic reaction. Recently introduced capsosomes, polymer capsules containing thousands of liposomes, are a promising platform for the creation of artificial cells. Capsosomes are formed by sequentially layering liposomes and polymers onto particle templates, followed by removal of the template cores. Herein, we engineer advanced capsosomes and demonstrate the ability to control the number of subcompartments and hence the degree of cargo loading. To achieve this, we employ a range of polymer separation layers and liposomes to form functional capsosomes comprising multiple layers of enzyme-loaded liposomes. Differences in conversion rates of an enzymatic assay are used to verify that multilayers of intact enzyme-loaded liposomes are assembled within a polymer hydrogel capsule. The size-dependent retention of the cargo encapsulated within the liposomal subcompartments during capsosome assembly and its dependence on environmental pH changes are also examined. We further show that temperature can be used to trigger an enzymatic reaction at the phase transition temperature of the liposomal subcompartments, and that the encapsulated enzymes can be utilized repeatedly in several subsequent conversions. These engineered capsosomes with tailored properties present new opportunities en route to the development of functional artificial cells.

  9. Feasibility Study of Cargo Airship Transportation Systems Powered by New Green Energy Technologies

    Science.gov (United States)

    Skuza, Jonathan R.; Park, Yeonjoon; Kim, Hyun Jung; Seaman, Shane T.; King, Glen C.; Choi, Sang H.; Song, Kyo D.; Yoon, Hargsoon; Lee, Kunik

    2014-01-01

    The development of transportation systems that use new and sustainable energy technologies is of utmost importance due to the possible future shortfalls that current transportation modes will encounter because of increased volume and costs. The introduction and further research and development of new transportation and energy systems by materials researchers at the National Aeronautics and Space Administration (NASA) Langley Research Center (LaRC) and the Department of Transportation are discussed in this Technical Memorandum. In this preliminary study, airship concepts were assessed for cargo transportation using various green energy technologies capable of 24-hour operation (i.e., night and day). Two prototype airships were successfully constructed and tested at LaRC to demonstrate their feasibility: one with commercially available solar cells for operation during the daytime and one with microwave rectennas (i.e., rectifying antennas) developed in-house for night-time operation. The test results indicate the feasibility of a cargo transportation airship powered by new green energy sources and wireless power technology. Future applications will exploit new green energy sources that use materials and devices recently developed or are in the process of being developed at LaRC. These include quantum well SiGe solar cells; low, mid-, and high temperature thermoelectric modules; and wireless microwave and optical rectenna devices. This study examines the need and development of new energy sources for transportation, including the current status of research, materials, and potential applications.

  10. Modulation of cargo release from dense core granules by size and actin network.

    Science.gov (United States)

    Felmy, Felix

    2007-08-01

    During regulated fusion of secretory granules with the plasma membrane, a fusion pore first opens and then dilates. The dilating pore allows cargo proteins from the dense core to be released into the extracellular space. Using real-time evanescent field fluorescence microscopy of live PC12 cells, it was determined how rapidly proteins of different sizes escape from single granules after fusion. Tissue plasminogen activator (tPA)-Venus is released 40-fold slower than the three times smaller neuropeptide Y [NPY-monomeric GFP (mGFP)]. An NPY bearing two mGFPs in tandem [NPY-(mGFP)(2)] as an intermediate-sized fusion probe is released most slowly. Although, the time-course of release varies substantially for a given probe. Coexpression of beta-actin, actin-related protein 3 or mAbp1 slowed the release of the two larger cargo molecules but did not affect release of NPY-mGFP or of the granule-membrane-bound probe Vamp-pHluorin. Additionally, high concentrations of cytochalasin D slowed release of the tPA-Venus. Together these results suggest that fusion pore dilation is not the only determinate of release time-course and that actin rearrangements similar to those mediating actin-mediated motility influences the time-course of release without directly interfering with the granule membrane to cell membrane connection.

  11. Indications of marine bioinvasion from network theory. An analysis of the global cargo ship network

    Science.gov (United States)

    Kölzsch, A.; Blasius, B.

    2011-12-01

    The transport of huge amounts of small aquatic organisms in the ballast tanks and at the hull of large cargo ships leads to ever increasing rates of marine bioinvasion. In this study, we apply a network theoretic approach to examine the introduction of invasive species into new ports by global shipping. This is the first stage of the invasion process where it is still possible to intervene with regulating measures. We compile a selection of widely used and newly developed network properties and apply these to analyse the structure and spread characteristics of the directed and weighted global cargo ship network (GCSN). Our results reveal that the GCSN is highly efficient, shows small world characteristics and is positive assortative, indicating that quick spread of invasive organisms between ports is likely. The GCSN shows strong community structure and contains two large communities, the Atlantic and Pacific trading groups. Ports that appear as connector hubs and are of high centralities are the Suez and Panama Canal, Singapore and Shanghai. Furthermore, from robustness analyses and the network's percolation behaviour, we evaluate differences of onboard and in-port ballast water treatment, set them into context with previous studies and advise bioinvasion management strategies.

  12. Life Science on the International Space Station Using the Next Generation of Cargo Vehicles

    Science.gov (United States)

    Robinson, J. A.; Phillion, J. P.; Hart, A. T.; Comella, J.; Edeen, M.; Ruttley, T. M.

    2011-01-01

    With the retirement of the Space Shuttle and the transition of the International Space Station (ISS) from assembly to full laboratory capabilities, the opportunity to perform life science research in space has increased dramatically, while the operational considerations associated with transportation of the experiments has changed dramatically. US researchers have allocations on the European Automated Transfer Vehicle (ATV) and Japanese H-II Transfer Vehicle (HTV). In addition, the International Space Station (ISS) Cargo Resupply Services (CRS) contract will provide consumables and payloads to and from the ISS via the unmanned SpaceX (offers launch and return capabilities) and Orbital (offers only launch capabilities) resupply vehicles. Early requirements drove the capabilities of the vehicle providers; however, many other engineering considerations affect the actual design and operations plans. To better enable the use of the International Space Station as a National Laboratory, ground and on-orbit facility development can augment the vehicle capabilities to better support needs for cell biology, animal research, and conditioned sample return. NASA Life scientists with experience launching research on the space shuttle can find the trades between the capabilities of the many different vehicles to be confusing. In this presentation we will summarize vehicle and associated ground processing capabilities as well as key concepts of operations for different types of life sciences research being launched in the cargo vehicles. We will provide the latest status of vehicle capabilities and support hardware and facilities development being made to enable the broadest implementation of life sciences research on the ISS.

  13. Cargo Commercial Orbital Transportation Services Environmental Control and Life Support Integration

    Science.gov (United States)

    Duchesne, Stephanie; Thacker, Karen; Williams, Dave

    2012-01-01

    The International Space Station s (ISS) largest crew and cargo resupply vehicle, the Space Shuttle, retired in 2011. To help augment ISS resupply and return capability, NASA announced a project to promote the development of Commercial Orbital Transportation Services (COTS) for the ISS in January of 2006. By December of 2008, NASA entered into space act agreements with SpaceX and Orbital Sciences Corporation for COTS development and ISS Commercial Resupply Services (CRS). The intent of CRS is to fly multiple resupply missions each year to ISS with SpaceX s Dragon vehicle providing resupply and return capabilities and Orbital Science Corporation s Cygnus vehicle providing resupply capability to ISS. The ISS program launched an integration effort to ensure that these new commercial vehicles met the requirements of the ISS vehicle and ISS program needs. The Environmental Control and Life Support System (ECLSS) requirements cover basic cargo vehicle needs including maintaining atmosphere, providing atmosphere circulation, and fire detection and suppression. The ISS-COTS integration effort brought unique challenges combining NASA s established processes and design knowledge with the commercial companies new initiatives and limited experience with human space flight. This paper will discuss the ISS ECLS COTS integration effort including challenges, successes, and lessons learned.

  14. Effect of catch bonding on transport of cellular cargo by dynein motors

    Science.gov (United States)

    Nair, Anil; Chandel, Sameep; Mitra, Mithun K.; Muhuri, Sudipto; Chaudhuri, Abhishek

    2016-09-01

    Recent experiments have demonstrated that dynein motors exhibit catch bonding behavior, in which the unbinding rate of a single dynein decreases with increasing force, for a certain range of force. Motivated by these experiments, we study the effect of catch bonding on unidirectional transport properties of cellular cargo carried by multiple dynein motors. We introduce a threshold force bond deformation (TFBD) model, consistent with the experiments, wherein catch bonding sets in beyond a critical applied load force. We find catch bonding can result in dramatic changes in the transport properties, which are in sharp contrast to kinesin-driven unidirectional transport, where catch bonding is absent. We predict that under certain conditions, the average velocity of the cellular cargo can actually increase as applied load is increased. We characterize the transport properties in terms of a velocity profile plot in the parameter space of the catch bond strength and the stall force of the motor. This plot yields predictions that may be experimentally accessed by suitable modifications of motor transport and binding properties.

  15. Development of Decision Making Algorithm for Control of Sea Cargo Containers by ``TAGGED'' Neutron Method

    Science.gov (United States)

    Anan'ev, A. A.; Belichenko, S. G.; Bogolyubov, E. P.; Bochkarev, O. V.; Petrov, E. V.; Polishchuk, A. M.; Udaltsov, A. Yu.

    2009-12-01

    Nowadays in Russia and abroad there are several groups of scientists, engaged in development of systems based on "tagged" neutron method (API method) and intended for detection of dangerous materials, including high explosives (HE). Particular attention is paid to possibility of detection of dangerous objects inside a sea cargo container. Energy gamma-spectrum, registered from object under inspection is used for determination of oxygen/carbon and nitrogen/carbon chemical ratios, according to which dangerous object is distinguished from not dangerous one. Material of filled container, however, gives rise to additional effects of rescattering and moderation of 14 MeV primary neutrons of generator, attenuation of secondary gamma-radiation from reactions of inelastic neutron scattering on objects under inspection. These effects lead to distortion of energy gamma-response from examined object and therefore prevent correct recognition of chemical ratios. These difficulties are taken into account in analytical method, presented in the paper. Method has been validated against experimental data, obtained by the system for HE detection in sea cargo, based on API method and developed in VNIIA. Influence of shielding materials on results of HE detection and identification is considered. Wood and iron were used as shielding materials. Results of method application for analysis of experimental data on HE simulator measurement (tetryl, trotyl, hexogen) are presented.

  16. Defective nuclear import of Tpr in Progeria reflects the Ran sensitivity of large cargo transport.

    Science.gov (United States)

    Snow, Chelsi J; Dar, Ashraf; Dutta, Anindya; Kehlenbach, Ralph H; Paschal, Bryce M

    2013-05-13

    The RanGTPase acts as a master regulator of nucleocytoplasmic transport by controlling assembly and disassembly of nuclear transport complexes. RanGTP is required in the nucleus to release nuclear localization signal (NLS)-containing cargo from import receptors, and, under steady-state conditions, Ran is highly concentrated in the nucleus. We previously showed the nuclear/cytoplasmic Ran distribution is disrupted in Hutchinson-Gilford Progeria syndrome (HGPS) fibroblasts that express the Progerin form of lamin A, causing a major defect in nuclear import of the protein, translocated promoter region (Tpr). In this paper, we show that Tpr import was mediated by the most abundant import receptor, KPNA2, which binds the bipartite NLS in Tpr with nanomolar affinity. Analyses including NLS swapping revealed Progerin did not cause global inhibition of nuclear import. Rather, Progerin inhibited Tpr import because transport of large protein cargoes was sensitive to changes in the Ran nuclear/cytoplasmic distribution that occurred in HGPS. We propose that defective import of large protein complexes with important roles in nuclear function may contribute to disease-associated phenotypes in Progeria.

  17. Harnessing a high cargo-capacity transposon for genetic applications in vertebrates.

    Directory of Open Access Journals (Sweden)

    Darius Balciunas

    2006-11-01

    Full Text Available Viruses and transposons are efficient tools for permanently delivering foreign DNA into vertebrate genomes but exhibit diminished activity when cargo exceeds 8 kilobases (kb. This size restriction limits their molecular genetic and biotechnological utility, such as numerous therapeutically relevant genes that exceed 8 kb in size. Furthermore, a greater payload capacity vector would accommodate more sophisticated cis cargo designs to modulate the expression and mutagenic risk of these molecular therapeutics. We show that the Tol2 transposon can efficiently integrate DNA sequences larger than 10 kb into human cells. We characterize minimal sequences necessary for transposition (miniTol2 in vivo in zebrafish and in vitro in human cells. Both the 8.5-kb Tol2 transposon and 5.8-kb miniTol2 engineered elements readily function to revert the deficiency of fumarylacetoacetate hydrolase in an animal model of hereditary tyrosinemia type 1. Together, Tol2 provides a novel nonviral vector for the delivery of large genetic payloads for gene therapy and other transgenic applications.

  18. Simultaneous spectroscopic measurements of the interior temperature and induced cargo release from pore-restricted mesoporous silica nanoparticles

    Science.gov (United States)

    Dong, Juyao; Zink, Jeffrey I.

    2016-05-01

    Temperature changes initiated within nano structures are being increasingly used to externally activate responsive delivery vehicles. Yet, the precise measurement of the nano environment temperature increase and its correlation with the induced macroscopic cargo release are difficult to achieve. In this study, we focus on a photothermally activated drug delivery system based on mesoporous silica nanoparticles, and use an optical nanothermometer - NaYF4:Yb3+,Er3+ crystals - for a ratiometric temperature measurement. Using fluorescent dyes as the payload molecule, both the nanoparticle interior temperature change and the macroscopic cargo release amount are monitored simultaneously by fluorescent spectroscopy. We found that the cargo release lags the temperature increase by about 5 min, revealing the threshold temperature that the particles have to reach before a substantial release could happen. Using this spectroscopic method, we are able to directly compare and correlate a nano environment event with its stimulated macroscopic results.Temperature changes initiated within nano structures are being increasingly used to externally activate responsive delivery vehicles. Yet, the precise measurement of the nano environment temperature increase and its correlation with the induced macroscopic cargo release are difficult to achieve. In this study, we focus on a photothermally activated drug delivery system based on mesoporous silica nanoparticles, and use an optical nanothermometer - NaYF4:Yb3+,Er3+ crystals - for a ratiometric temperature measurement. Using fluorescent dyes as the payload molecule, both the nanoparticle interior temperature change and the macroscopic cargo release amount are monitored simultaneously by fluorescent spectroscopy. We found that the cargo release lags the temperature increase by about 5 min, revealing the threshold temperature that the particles have to reach before a substantial release could happen. Using this spectroscopic method, we are

  19. Biological Activities of Extracellular Vesicles and Their Cargos from Bovine and Human Milk in Humans and Implications for Infants.

    Science.gov (United States)

    Zempleni, Janos; Aguilar-Lozano, Ana; Sadri, Mahrou; Sukreet, Sonal; Manca, Sonia; Wu, Di; Zhou, Fang; Mutai, Ezra

    2017-01-01

    Extracellular vesicles (EVs) in milk harbor a variety of compounds, including lipids, proteins, noncoding RNAs, and mRNAs. Among the various classes of EVs, exosomes are of particular interest, because cargo sorting in exosomes is a regulated, nonrandom process and exosomes play essential roles in cell-to-cell communication. Encapsulation in exosomes confers protection against enzymatic and nonenzymatic degradation of cargos and provides a pathway for cellular uptake of cargos by endocytosis of exosomes. Compelling evidence suggests that exosomes in bovine milk are transported by intestinal cells, vascular endothelial cells, and macrophages in human and rodent cell cultures, and bovine-milk exosomes are delivered to peripheral tissues in mice. Evidence also suggests that cargos in bovine-milk exosomes, in particular RNAs, are delivered to circulating immune cells in humans. Some microRNAs and mRNAs in bovine-milk exosomes may regulate the expression of human genes and be translated into protein, respectively. Some exosome cargos are quantitatively minor in the diet compared with endogenous synthesis. However, noncanonical pathways have been identified through which low concentrations of dietary microRNAs may alter gene expression, such as the accumulation of exosomes in the immune cell microenvironment and the binding of microRNAs to Toll-like receptors. Phenotypes observed in infant-feeding studies include higher Mental Developmental Index, Psychomotor Development Index, and Preschool Language Scale-3 scores in breastfed infants than in those fed various formulas. In mice, supplementation with plant-derived MIR-2911 improved the antiviral response compared with controls. Porcine-milk exosomes promote the proliferation of intestinal cells in mice. This article discusses the above-mentioned advances in research concerning milk exosomes and their cargos in human nutrition. Implications for infant nutrition are emphasized, where permitted, but data in infants are

  20. Design and application of radiation apparatus for sup 6 sup 0 Co cargo train on-line inspection system

    CERN Document Server

    Wu Zhi Fang; Zhang Yuai

    2002-01-01

    Based on the special requirement for radiation apparatus of sup 6 sup 0 Co cargo train on-line inspection system, a radiation apparatus including two-level shutter, working container, storing container and electromagnetism are designed. The makeup, working mode and functional realization of the radiation apparatus are introduced. The system is used in Manzhouli customs cargo train on-line inspection system. The practice shows that the radiation apparatus is reliable to work steadily and the operating speed of the main shutter can reach 0.1 s open and 0.15 s close

  1. 49 CFR 172.331 - Bulk packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Bulk packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks. 172.331 Section 172.331 Transportation Other Regulations... packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks. (a) Each...

  2. 49 CFR 174.63 - Portable tanks, IM portable tanks, IBCs, Large Packagings, cargo tanks, and multi-unit tank car...

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Portable tanks, IM portable tanks, IBCs, Large Packagings, cargo tanks, and multi-unit tank car tanks. 174.63 Section 174.63 Transportation Other....63 Portable tanks, IM portable tanks, IBCs, Large Packagings, cargo tanks, and multi-unit tank...

  3. Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis.

    Science.gov (United States)

    Dutta, Dipannita; Donaldson, Julie G

    2015-09-01

    Clathrin-mediated endocytosis (CME) and clathrin-independent endocytosis (CIE) co-exist in most cells but little is known about their communication and coordination. Here we show that when CME was inhibited, endocytosis by CIE continued but endosomal trafficking of CIE cargo proteins was altered. CIE cargo proteins that normally traffic directly into Arf6-associated tubules after internalization and avoid degradation (CD44, CD98 and CD147) now trafficked to lysosomes and were degraded. The endosomal tubules were also absent and Arf6-GTP levels were elevated. The altered trafficking, loss of the tubular endosomal network and elevated Arf6-GTP levels caused by inhibition of CME were rescued by expression of Rab35, a Rab associated with clathrin-coated vesicles, or its effector ACAPs, Arf6 GTPase activating proteins (GAP) that inactivate Arf6. Furthermore, siRNA knockdown of Rab35 recreated the phenotype of CME ablation on CIE cargo trafficking without altering endocytosis of transferrin. These observations suggest that Rab35 serves as a CME detector and that loss of CME, or Rab35 input, leads to elevated Arf6-GTP and shifts the sorting of CIE cargo proteins to lysosomes and degradation.

  4. 31 CFR 585.215 - Conveyances and cargo suspected of being in violation of United Nations sanctions; detention...

    Science.gov (United States)

    2010-07-01

    ... being in violation of United Nations sanctions; detention; blocking. 585.215 Section 585.215 Money and... and cargo suspected of being in violation of United Nations sanctions; detention; blocking. (a) Except... § 585.201, but which are suspected of a violation of United Nations Security Council Resolutions No....

  5. Molecular Recognition of PTS-1 Cargo Proteins by Pex5p: Implications for Protein Mistargeting in Primary Hyperoxaluria

    Directory of Open Access Journals (Sweden)

    Noel Mesa-Torres

    2015-02-01

    Full Text Available Peroxisomal biogenesis and function critically depends on the import of cytosolic proteins carrying a PTS1 sequence into this organelle upon interaction with the peroxin Pex5p. Recent structural studies have provided important insights into the molecular recognition of cargo proteins by Pex5p. Peroxisomal import is a key feature in the pathogenesis of primary hyperoxaluria type 1 (PH1, where alanine:glyoxylate aminotransferase (AGT undergoes mitochondrial mistargeting in about a third of patients. Here, we study the molecular recognition of PTS1 cargo proteins by Pex5p using oligopeptides and AGT variants bearing different natural PTS1 sequences, and employing an array of biophysical, computational and cell biology techniques. Changes in affinity for Pex5p (spanning over 3–4 orders of magnitude reflect different thermodynamic signatures, but overall bury similar amounts of molecular surface. Structure/energetic analyses provide information on the contribution of ancillary regions and the conformational changes induced in Pex5p and the PTS1 cargo upon complex formation. Pex5p stability in vitro is enhanced upon cargo binding according to their binding affinities. Moreover, we provide evidence that the rational modulation of the AGT: Pex5p binding affinity might be useful tools to investigate mistargeting and misfolding in PH1 by pulling the folding equilibria towards the native and peroxisomal import competent state.

  6. 76 FR 69271 - Notice of Inquiry; U.S. Inland Containerized Cargo Moving Through Canadian and Mexican Seaports

    Science.gov (United States)

    2011-11-08

    ... Notice of Inquiry; U.S. Inland Containerized Cargo Moving Through Canadian and Mexican Seaports AGENCY... Canadian and Mexican seaports. DATES: Comments are due on or before December 22, 2011. ADDRESSES: Submit... the expanded Mexican port L zaro C rdenas. These same years saw investment in and promotion...

  7. Pick-up, transport and release of a molecular cargo using a small-molecule robotic arm

    Science.gov (United States)

    Kassem, Salma; Lee, Alan T. L.; Leigh, David A.; Markevicius, Augustinas; Solà, Jordi

    2016-02-01

    Modern-day factory assembly lines often feature robots that pick up, reposition and connect components in a programmed manner. The idea of manipulating molecular fragments in a similar way has to date only been explored using biological building blocks (specifically DNA). Here, we report on a wholly artificial small-molecule robotic arm capable of selectively transporting a molecular cargo in either direction between two spatially distinct, chemically similar, sites on a molecular platform. The arm picks up/releases a 3-mercaptopropanehydrazide cargo by formation/breakage of a disulfide bond, while dynamic hydrazone chemistry controls the cargo binding to the platform. Transport is controlled by selectively inducing conformational and configurational changes within an embedded hydrazone rotary switch that steers the robotic arm. In a three-stage operation, 79-85% of 3-mercaptopropanehydrazide molecules are transported in either (chosen) direction between the two platform sites, without the cargo at any time fully dissociating from the machine nor exchanging with other molecules in the bulk.

  8. 33 CFR Appendix A to Part 157 - Damage Assumptions, Hypothetical Outflows, and Cargo Tank Size and Arrangements

    Science.gov (United States)

    2010-07-01

    ... Outflows, and Cargo Tank Size and Arrangements A Appendix A to Part 157 Navigation and Navigable Waters... MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Pt. 157, App. A Appendix A to Part 157... from tank vessels, three dimensions of the extent of damage of a parallelepiped on the side and...

  9. Significant role of PB1 and UBA domains in multimerization of Joka2, a selective autophagy cargo receptor from tobacco

    Directory of Open Access Journals (Sweden)

    Katarzyna eZientara-Rytter

    2014-01-01

    Full Text Available Tobacco Joka2 protein is a hybrid homolog of two mammalian selective autophagy cargo receptors, p62 and NBR1. These proteins can directly interact with the members of ATG8 family and the polyubiquitinated cargoes designed for degradation. Function of the selective autophagy cargo receptors relies on their ability to form protein aggregates. It has been shown that the N-terminal PB1 domain of p62 is involved in formation of aggregates, while the UBA domains of p62 and NBR1 have been associated mainly with cargo binding. Here we focus on roles of PB1 and UBA domains in localization and aggregation of Joka2 in plant cells. We show that Joka2 can homodimerize not only through its N-terminal PB1-PB1 interactions but also via interaction between N-terminal PB1 and C-terminal UBA domains. We also demonstrate that Joka2 co-localizes with recombinant ubiquitin and sequestrates it into aggregates and that C-terminal part (containing UBA domains is sufficient for this effect. Our results indicate that Joka2 accumulates in cytoplasmic aggregates and suggest that in addition to these multimeric forms it also exists in the nucleus and cytoplasm in a monomeric form.

  10. United Airlines Assists Tsunami Relief Effort United donates cargo space for relief supplies going to southern Asia

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ United Airlines today announced several initiatives to assist with the tsunami relief effort in Asia, including the donation of available cargo space to ship relief supplies to southern Thailand,Indonesia, Sri Lanka and other coastal areas on the Indian Ocean.

  11. United Airlines Assists Tsunami Relief Effort United donates cargo space for relief supplies going to southern Asia

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

      United Airlines today announced several initiatives to assist with the tsunami relief effort in Asia, including the donation of available cargo space to ship relief supplies to southern Thailand,Indonesia, Sri Lanka and other coastal areas on the Indian Ocean.……

  12. 76 FR 6178 - Deadline for Notification of Intent to Use the Airport Improvement Program (AIP) Primary, Cargo...

    Science.gov (United States)

    2011-02-03

    ... [Federal Register Volume 76, Number 23 (Thursday, February 3, 2011)] [Notices] [Page 6178] [FR Doc No: 2011-2381] DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Deadline for Notification of Intent to Use the Airport Improvement Program (AIP) Primary, Cargo, and Nonprimary...

  13. Low cost, robust and real time system for detecting and tracking moving objects to automate cargo handling in port terminals

    NARCIS (Netherlands)

    Vaquero, V.; Repiso, E.; Sanfeliu, A.; Vissers, J.; Kwakkernaat, M.

    2016-01-01

    The presented paper addresses the problem of detecting and tracking moving objects for autonomous cargo handling in port terminals using a perception system which input data is a single layer laser scanner. A computationally low cost and robust Detection and Tracking Moving Objects (DATMO) algorithm

  14. Molecular motors transporting cargos in viscoelastic cytosol: how to beat subdiffusion with a power stroke?

    CERN Document Server

    Goychuk, Igor; Metzler, Ralf

    2013-01-01

    Anomalously slow passive diffusion, $\\langle \\delta x^2(t)\\rangle\\simeq t^{\\alpha}$, with $0<\\alpha<1$, of larger tracers such as messenger RNA and endogenous submicron granules in the cytoplasm of living biological cells has been demonstrated in a number of experiments and has been attributed to the viscoelastic physical nature of the cellular cytoplasm. This finding provokes the question to which extent active intracellular transport is affected by this viscoelastic environment: does the subdiffusion of free submicron cargo such as vesicles and organelles always imply anomalously slow transport by molecular motors such as kinesins, that is, directed transport characterized by a sublinear growth of the mean distance, $\\langle x(t)\\rangle\\simeq t^{\\alpha_{\\rm eff}}$, with $0<\\alpha_{\\rm eff}<1$? Here we study a generic model approach combining the commonly accepted two-state Brownian ratchet model of kinesin motors based on the continuous-state diffusion along microtubule driven by a flashing bind...

  15. Theranostic applications of carbon nanomaterials in cancer: Focus on imaging and cargo delivery.

    Science.gov (United States)

    Chen, Daiqin; Dougherty, Casey A; Zhu, Kaicheng; Hong, Hao

    2015-07-28

    Carbon based nanomaterials have attracted significant attention over the past decades due to their unique physical properties, versatile functionalization chemistry, and biological compatibility. In this review, we will summarize the current state-of-the-art applications of carbon nanomaterials in cancer imaging and drug delivery/therapy. The carbon nanomaterials will be categorized into fullerenes, nanotubes, nanohorns, nanodiamonds, nanodots and graphene derivatives based on their morphologies. The chemical conjugation/functionalization strategies of each category will be introduced before focusing on their applications in cancer imaging (fluorescence/bioluminescence, magnetic resonance (MR), positron emission tomography (PET), single-photon emission computed tomography (SPECT), photoacoustic, Raman imaging, etc.) and cargo (chemo/gene/therapy) delivery. The advantages and limitations of each category and the potential clinical utilization of these carbon nanomaterials will be discussed. Multifunctional carbon nanoplatforms have the potential to serve as optimal candidates for image-guided delivery vectors for cancer.

  16. Ligand binding strategies of human serum albumin: how can the cargo be utilized?

    Science.gov (United States)

    Varshney, Ankita; Sen, Priyankar; Ahmad, Ejaz; Rehan, Mohd; Subbarao, Naidu; Khan, Rizwan Hasan

    2010-01-01

    Human serum albumin (HSA), being the most abundant carrier protein in blood and a modern day clinical tool for drug delivery, attracts high attention among biologists. Hence, its unfolding/refolding strategies and exogenous/endogenous ligand binding preference are of immense use in therapeutics and clinical biochemistry. Among its fellow proteins albumin is known to carry almost every small molecule. Thus, it is a potential contender for being a molecular cargo/or nanovehicle for clinical, biophysical and industrial purposes. Nonetheless, its structure and function are largely regulated by various chemical and physical factors to accommodate HSA to its functional purpose. This multifunctional protein also possesses enzymatic properties which may be used to convert prodrugs to active therapeutics. This review aims to highlight current overview on the binding strategies of protein to various ligands that may be expected to lead to significant clinical applications.

  17. Trajectory design and navigation analysis for Cargo Transfer Vehicle proximity operations

    Science.gov (United States)

    Marsh, S. M.; White, B. D.

    1992-08-01

    The paper documents an investigation of alternative (or off-nominal) final approach trajectories and terminal guidance algorithms for Cargo Transfer Vehicle (CTV) automated rendezvous and proximity operation in the vicinity of Space Station Freedom (SSF). Ten candidate trajectory designs, representing a survey of all approaches that reasonably satisfy the mission constraints, are compared qualitatively and quantitatively. It is shown that several fundamentally different types of approach strategies are feasible in the CTV application and enjoy more favorable passive collision avoidance and plume impingement properties than the nominal. They are, however, generally more costly in propellant. The extent to which an alternative approach is more costly is determined largely by the abort (or specifically the 'hold' or 'wave-off') requirements. These approach techniques are verified in a software simulation using a parametrically defined candidate navigation system, and proximity sensor accuracy requirements are derived.

  18. Analysis and Design of a Long Range PTFE Substrate UHF RFID Tag for Cargo Container Identification

    Science.gov (United States)

    Petrariu, Adrian-Ioan; Popa, Valentin

    2016-01-01

    In this paper, a high-performances microstrip antenna for UHF (ultra high frequency) RFID (radio frequency identification) tag is designed, prototyped and tested. The antenna consists of two main components: a 1.52 mm RT/duroid 5880 laminate substrate on which the antenna is designed and a 10 mm polytetrafluoroethylene (PTFE) dielectric material placed as a separator between the antenna and the reference ground plane for the microstrip antenna. With this structure, the RFID tag can reach a maximum reading distance of 19 m, although the antenna has a compact size of 80 mm × 50 mm. The long reading distance is obtained by attaching to the antenna an RFID chip that can provide a reading sensitivity of -20.5 dBm. The high bandwidth from 677 MHz to 947 MHz measured at -10 dB, makes the tag being usable worldwide especially for cargo container identification, the main purpose of this research.

  19. RESEARCH ACTION: IMPLEMENTATION ZERO BASED BUDGET (ZBB IN THE PROVIDER SERVICE LEASING EQUIPAMENTS OF CARGO HANDLING.

    Directory of Open Access Journals (Sweden)

    Levi Gimenez

    2013-06-01

    Full Text Available This article aims to present the implementation of zero-based budgeting in a rental service provider of cargo handling equipment using the action research methodology. The goal was to examine the possibility of concomitant use of this instrument in service providers in need of accurate information that enables targeting at the best result in a setting avid for quick decisions and actions. Action research was used as research method. It was concluded that it is suitable for this branch, confirming its position as a useful model for restructuring and cutting costs, improving operational and financial results, and as a factor improving organizational environment (behavioral aspects, indirectly creating value to stakeholders.

  20. Trajectory design and navigation analysis for Cargo Transfer Vehicle proximity operations

    Science.gov (United States)

    Marsh, S. M.; White, B. D.

    1992-01-01

    The paper documents an investigation of alternative (or off-nominal) final approach trajectories and terminal guidance algorithms for Cargo Transfer Vehicle (CTV) automated rendezvous and proximity operation in the vicinity of Space Station Freedom (SSF). Ten candidate trajectory designs, representing a survey of all approaches that reasonably satisfy the mission constraints, are compared qualitatively and quantitatively. It is shown that several fundamentally different types of approach strategies are feasible in the CTV application and enjoy more favorable passive collision avoidance and plume impingement properties than the nominal. They are, however, generally more costly in propellant. The extent to which an alternative approach is more costly is determined largely by the abort (or specifically the 'hold' or 'wave-off') requirements. These approach techniques are verified in a software simulation using a parametrically defined candidate navigation system, and proximity sensor accuracy requirements are derived.

  1. Design of an autonomous teleoperated cargo transporting vehicle for lunar base operations

    Science.gov (United States)

    Holt, James; Lao, Tom; Monali, Nkoy

    1989-01-01

    At the turn of the century NASA plans to begin construction of a lunar base. The base will likely consist of developed areas (i.e., habitation, laboratory, landing and launching sites, power plant) separated from each other due to safety considerations. The Self-Repositioning Track Vehicle (SRTV) was designed to transport cargo between these base facilities. The SRTV operates by using two robotic arms to raise and position segments of track upon which the vehicle travels. The SRTV utilizes the semiautonomous mobility (SAM) method of teleoperation; actuator-controlled interlocking track sections; two robotic arms each with five degrees of freedom; and these materials: titanium for structural members and aluminum for shell members, with the possible use of light-weight, high-strength composites.

  2. The Migration Experience as It Relates to Cargo Participation in San Miguel Cuevas, Oaxaca

    Directory of Open Access Journals (Sweden)

    Silvia Ventura Luna

    2010-01-01

    Full Text Available Este artículo ofrece un recuento de los continuos efectos de la migración sobre el gobierno municipal y la organización de una comunidad mixteca: San Miguel Cuevas, Oaxaca, México. Legalmente constituida como un autogobierno municipal indígena, en décadas recientes, Cuevas ha sido reconstituida como una comunidad transnacional, cuyos ciudadanos radican principalmente en ella y en Fresno, California. La creciente circularidad y permanente migración de Cuevas a Estados Unidos desde finales de los ochenta, así como los usos y costumbres impuestos por la vida comunitaria en Cuevas, pone a sus varones migrantes en una situación complicada para cumplir sus obligaciones familiares y comunitarias -cívicas y ceremoniales (cargos-, que se requieren para mantener su ciudadanía y residencia en Cuevas y tener acceso a tierras comunales y otros recursos.

  3. Primary Beam Air Kerma Dependence on Distance from Cargo and People Scanners

    Energy Technology Data Exchange (ETDEWEB)

    Strom, Daniel J.; Cerra, Frank

    2016-06-01

    The distance dependence of air kerma or dose rate of the primary radiation beam is not obvious for security scanners of cargo and people in which there is relative motion between a collimated source and the person or object being imaged. To study this problem, one fixed line source and three moving-source scan-geometry cases are considered, each characterized by radiation emanating perpendicular to an axis. The cases are 1) a stationary line source of radioactive material, e.g., contaminated solution in a pipe; 2) a moving, uncollimated point source of radiation that is shuttered or off when it is stationary; 3) a moving, collimated point source of radiation that is shuttered or off when it is stationary; and 4) a translating, narrow “pencil” beam emanating in a flying-spot, raster pattern. Each case is considered for short and long distances compared to the line source length or path traversed by a moving source. The short distance model pertains mostly to dose to objects being scanned and personnel associated with the screening operation. The long distance model pertains mostly to potential dose to bystanders. For radionuclide sources, the number of nuclear transitions that occur a) per unit length of a line source, or b) during the traversal of a point source, is a unifying concept. The “universal source strength” of air kerma rate at a meter from the source can be used to describe x-ray machine or radionuclide sources. For many cargo and people scanners with highly collimated fan or pencil beams, dose varies as the inverse of the distance from the source in the near field and with the inverse square of the distance beyond a critical radius. Ignoring the inverse square dependence and using inverse distance dependence is conservative in the sense of tending to overestimate dose.

  4. Gestión de perfiles de cargos laborales basados en competencias

    Directory of Open Access Journals (Sweden)

    Franklin Sandoval

    2012-01-01

    Full Text Available El cambio vertiginoso que imponen los mercados, cada vez más globales y competitivos, necesariamente impacta en el desempeño de las organizaciones, donde el reto es mejorar su productividad; en este contexto surgen las competencias como un elemento base para la gestión del talento humano. La administración pública venezolana (APV no escapa de esta realidad; requiere mecanismos que apoyen una adecuada administración de las competencias laborales de sus empleados. Por esta razón, en este trabajo se desarrolla un modelo de gestión fundamentado en el conocimiento del dominio de las competencias laborales y perfiles de cargos, que estructura el proceso de gestión en tres actividades: 1 evaluación diagnóstica, 2 planeamiento y desarrollo de carrera, y 3 evaluación de desempeño. El enfoque metodológico utilizado en esta investigación fue proyectivo. Se obtuvo un modelo cíclico que promueve la revisión continua para la toma de decisiones y media entre los sub-sistemas de planificación estratégica y talento humano. Se concluye que una vez implementado el modelo, se podrán definir perfiles de cargos acordes a las expectativas de la organización, favoreciendo la productividad de sus empleados, propiciando rendimientos excelentes, así como la incorporación de mejoras y la concientización de los empleados orientada a la corresponsabilidad de su autodesarrollo

  5. Direct attachment of nanoparticle cargo to Salmonella typhimurium membranes designed for combination bacteriotherapy against tumors.

    Science.gov (United States)

    Kazmierczak, Robert; Choe, Elizabeth; Sinclair, Jared; Eisenstark, Abraham

    2015-01-01

    Nanoparticle technology is an emerging approach to resolve difficult-to-manage internal diseases. It is highly regarded, in particular, for medical use in treatment of cancer due to the innate ability of certain nanoparticles to accumulate in the porous environment of tumors and to be toxic to cancer cells. However, the therapeutic success of nanoparticles is limited by the technical difficulty of fully penetrating and thus attacking the tumor. Additionally, while nanoparticles possess seeming-specificity due to the unique physiological properties of tumors themselves, it is difficult to tailor the delivery of nanoparticles or drugs in other models, such as use in cardiac disease, to the specific target. Thus, a need for delivery systems that will accurately and precisely bring nanoparticles carrying drug payloads to their intended sites currently exists. Our solution to this engineering challenge is to load such nanoparticles onto a biological "mailman" (a novel, nontoxic, therapeutic strain of Salmonella typhimurium engineered to preferentially and precisely seek out, penetrate, and hinder prostate cancer cells as the biological delivery system) that will deliver the therapeutics to a target site. In this chapter, we describe two methods that establish proof-of-concept for our cargo loading and delivery system by attaching nanoparticles to the Salmonella membrane. The first method (Subheading 1.1) describes association of sucrose-conjugated gold nanoparticles to the surface of Salmonella bacteria. The second method (Subheading 1.2) biotinylates the native Salmonella membrane to attach streptavidin-conjugated fluorophores as example nanoparticle cargo, with an alternative method (expression of membrane bound biotin target sites using autodisplay plasmid vectors) that increases the concentration of biotin on the membrane surface for streptavidin-conjugated nanoparticle attachment. By directly attaching the fluorophores to our bacterial vector through biocompatible

  6. C-C chemokine receptor-7 mediated endocytosis of antibody cargoes into intact cells

    Directory of Open Access Journals (Sweden)

    Xavier eCharest-Morin

    2013-09-01

    Full Text Available The C-C chemokine receptor-7 (CCR7 is a G protein coupled receptor that has a role in leukocyte homing, but that is also expressed in aggressive tumor cells. Preclinical research supports that CCR7 is a valid target in oncology. In view of the increasing availability of therapeutic monoclonal antibodies that carry cytotoxic cargoes, we studied the feasibility of forcing intact cells to internalize known monoclonal antibodies by exploiting the cycle of endocytosis and recycling triggered by the CCR7 agonist CCL19. Firstly, an anti-CCR7 antibody (CD197; clone 150503 labeled surface recombinant CCR7 expressed in intact HEK 293a cells and the fluorescent antibody was internalized following CCL19 treatment. Secondly, a recombinant myc-tagged CCL19 construction was exploited along the anti-myc monoclonal antibody 4A6. The myc-tagged ligand was produced as a conditioned medium of transfected HEK 293a cells that contained the equivalent of 430 ng/ml of immunoreactive CCL19 (average value, ELISA determination. CCL19-myc, but not authentic CCL19, carried the fluorophore-labeled antibody 4A6 into other recipient cells that expressed recombinant CCR7 (microscopy, cytofluorometry. The immune complexes were apparent in endosomal structures, colocalized well with the small GTPase Rab5 and progressed toward Rab7-positive endosomes. A dominant negative form of Rab5 (GDP-locked inhibited this endocytosis. Further, endosomes in CCL19-myc- or CCL19-stimulated cells were positive for β-arrestin2, but rarely for β-arrestin1. Following treatment with CCL19-myc and the 4A6 antibody, the melanoma cell line A375 that expresses endogenous CCR7 was specifically stained using a secondary peroxidase-conjugated antibody. Agonist-stimulated CCR7 can transport antibody-based cargoes, with possible therapeutic applications in oncology.

  7. Basic Notion and Characteristics of Railway Transportation of Overweight Cargoes%铁路超重货物运输基本概念及特征分析

    Institute of Scientific and Technical Information of China (English)

    李笑红

    2012-01-01

    针对铁路超重货物的认识误区,对超重货物运输中相关概念的内涵及特征、超重产生的原因等进行了分析与探讨,同时也将超重货物与几个易混淆概念进行了比较.%In this paper, we analyzed the notion and characteristics of the overweight cargo transportation and the causes of the overweight cargoes and then compared the several confusing concepts related to overweight cargoes.

  8. Lipid Raft-Mediated Membrane Tethering and Delivery of Hydrophobic Cargos from Liquid Crystal-Based Nanocarriers.

    Science.gov (United States)

    Nag, Okhil K; Naciri, Jawad; Oh, Eunkeu; Spillmann, Christopher M; Delehanty, James B

    2016-04-20

    A main goal of bionanotechnology and nanoparticle (NP)-mediated drug delivery (NMDD) continues to be the development of novel biomaterials that can controllably modulate the activity of the NP-associated therapeutic cargo. One of the desired subcellular locations for targeted delivery in NMDD is the plasma membrane. However, the controlled delivery of hydrophobic cargos to the membrane bilayer poses significant challenges including cargo precipitation and lack of specificity. Here, we employ a liquid crystal NP (LCNP)-based delivery system for the controlled partitioning of a model dye cargo from within the NP core into the plasma membrane bilayer. During synthesis of the NPs, the water-insoluble model dye cargo, 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO), was efficiently incorporated into the hydrophobic LCNP core as confirmed by multiple spectroscopic analyses. Conjugation of a PEGylated cholesterol derivative to the NP surface (DiO-LCNP-PEG-Chol) facilitated the localization of the dye-loaded NPs to lipid raft microdomains in the plasma membrane in HEK 293T/17 cell. Analysis of DiO cellular internalization kinetics revealed that when delivered as a LCNP-PEG-Chol NP, the half-life of DiO membrane residence time (30 min) was twice that of free DiO (DiO(free)) (15 min) delivered from bulk solution. Time-resolved laser scanning confocal microscopy was employed to visualize the passive efflux of DiO from the LCNP core and its insertion into the plasma membrane bilayer as confirmed by Förster resonance energy transfer (FRET) imaging. Finally, the delivery of DiO as a LCNP-PEG-Chol complex resulted in the attenuation of its cytotoxicity; the NP form of DiO exhibited ∼30-40% less toxicity compared to DiO(free). Our data demonstrate the utility of the LCNP platform as an efficient vehicle for the combined membrane-targeted delivery and physicochemical modulation of molecular cargos using lipid raft-mediated tethering.

  9. FPSO - floating production storage and off loading -conversion or new build; FPSO - conversao ou construcao nova

    Energy Technology Data Exchange (ETDEWEB)

    Barbosa, Fernando Sampaio; Mitidieri, Jorge Luiz

    2004-07-01

    The FPSO technology over the past years has rapidly evolved from tanker based units with classical mooring system to purpose built vessels equipped with dynamic positioning assistance, sophisticated permanent turrets or disconnectable mooring systems. The growth in the number of FPSO's in the 1980's was largely based converted tankers but new-builds now represent 40% of the FPSO fleet. This paper intends to discuss the main technical and economical aspects to be considered when selecting the project for a FPSO construction, including, among others, general and structural conditions for the takers to be converted, safety considerations, systems to be installed and general analysis of engineering design. The study compares construction schedule and the time schedule influence on project conclusion. The evaluation of the designed life time of each vessel an its implications on the project cost and the importance on the decision is also evaluated. (author)

  10. Off-loading the diabetic foot for ulcer prevention and healing

    NARCIS (Netherlands)

    P.R. Cavanagh; S.A. Bus

    2010-01-01

    Background: Retrospective and prospective studies have shown that elevated plantar pressure is a causative factor in the development of many plantar ulcers in diabetic patients and that ulceration is often a precursor of lower extremity amputation. In this article, we review the evidence that reliev

  11. El sistema de prestaciones por hijo a cargo en España

    Directory of Open Access Journals (Sweden)

    Obiol Francés, Sandra

    2006-04-01

    Full Text Available Child Benefit (prestaciones familiares por hijo a cargo is the only monetary, regular and direct allowance to contribute bringing up children in Spain. Therefore, the Family Allowance System might be seen as a privileged watchtower where it is possible to observe complex and dynamic relationships between the family, the State and the market. Moreover, it shows its influence on the wellbeing and on the dynamics of inclusion or exclusion. This paper analyzes the Spanish economic allowance addressed to children and its evolution since its last reform in 1990. The main goal is to contribute to our understanding of the compromise level acquired by the Spanish State for familycare. This study tries to draw where the limit between public and private areas is and which are the differences concerning the cost of chid bearing and education.

    Las prestaciones por hijo a cargo constituyen en España la única prestación de carácter monetario, periódico y directo que se otorga a nivel estatal para contribuir a los gastos generados por la crianza y educación de los hijos/as. Este hecho convierte al sistema de prestaciones en una privilegiada atalaya para observar las complejas y dinámicas relaciones que se dan entre familia, Estado y mercado en la canalización del bienestar y, por consiguiente, en las dinámicas de inclusión/exclusión de este mismo bienestar. En este artículo se analiza la evolución del sistema español de prestaciones económicas dirigidas a los hijos desde su última gran reforma en 1990, con la intención de que su estudio nos ayude a dilucidar qué grado de compromiso adquiere el Estado respecto a la atención a la familia, en qué lugar sitúa el límite entre el ámbito público y el ámbito privado, y dónde se incluye, entre esta diferenciación de esferas, la asunción del coste de la crianza y la educación de los hijos.

  12. 46 CFR 32.55-30 - Venting of cargo tanks of tank vessels constructed between November 10, 1936, and July 1, 1951-TB...

    Science.gov (United States)

    2010-10-01

    ... to a reasonable height above the weather deck and be fitted with a flame arrester or pressure-vacuum... arrester or a pressure-vacuum relief valve. (d) Grade D or E liquids. Cargo tanks in which Grade D or...

  13. 民航客机客货舱电缆设计%Civil Airplane Wiring Design for Cabin and Cargo Zone

    Institute of Scientific and Technical Information of China (English)

    陈志达

    2015-01-01

    Referring to the specialty of civil airplane’ s cabin and cargo zone, this article has introduced the basic method of wir-ing design for cabin and cargo zone, which includes the main wiring channel design of cabin and cargo zone, the wiring channel disconnect design of cabin and cargo zone, door wiring design, bilge wiring.%结合民航客机客货舱的特点,介绍了客货舱电缆设计的基本方法,包括客货舱电缆主通道设计、客货舱电缆分离面设计、舱门电缆设计、舱底布线。

  14. Analysis of Arf1 GTPase-dependent membrane binding and remodeling using the exomer secretory vesicle cargo adaptor

    Science.gov (United States)

    Paczkowski, Jon E.; Fromme, J. Christopher

    2016-01-01

    Summary Protein-protein and protein-membrane interactions play a critical role in shaping biological membranes through direct physical contact with the membrane surface. This is particularly evident in many steps of membrane trafficking, in which proteins deform the membrane and induce fission to form transport carriers. The small GTPase Arf1 and related proteins have the ability to remodel membranes by insertion of an amphipathic helix into the membrane. Arf1 and the exomer cargo adaptor coordinate cargo sorting into subset of secretory vesicle carriers in the model organism Saccharomyces cerevisiae. Here, we detail the assays we used to explore the cooperative action of Arf1 and exomer to bind and remodel membranes. We expect these methods are broadly applicable to other small GTPase/effector systems where investigation of membrane binding and remodeling is of interest. PMID:27632000

  15. Intentional cargo disruption by nefarious means: Examining threats, systemic vulnerabilities and securitisation measures in complex global supply chains.

    Science.gov (United States)

    McGreevy, Conor; Harrop, Wayne

    2015-01-01

    Global trade and commerce requires products to be securely contained and transferred in a timely way across great distances and between national boundaries. Throughout the process, cargo and containers are stored, handled and checked by a range of authorities and authorised agents. Intermodal transportation involves the use of container ships, planes, railway systems, land bridges, road networks and barges. This paper examines the the nefarious nature of intentional disruption and nefarious risks associated with the movement of cargo and container freight. The paper explores main threats, vulnerabilities and security measures relevant to significant intermodal transit risk issues such as theft, piracy, terrorism, contamination, counterfeiting and product tampering. Three risk and vulnerability models are examined and basic standards and regulations that are relevant to safe and secure transit of container goods across international supply networks are outlined.

  16. ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.

    Science.gov (United States)

    Edelmann, Franziska T; Niedner, Annika; Niessing, Dierk

    2015-01-01

    Asymmetric ASH1 mRNA transport during mitosis of budding yeast constitutes one of the best-studied examples of mRNA localization. Recently, 2 studies used in vitro motility assays to prove that motile ASH1 mRNA-transport complexes can be reconstituted entirely from recombinant factors. Both studies, however, differed in their conclusions on whether cargo RNA itself is required for particle assembly and thus activation of directional transport. Here we provide direct evidence that stable complexes do assemble in absence of RNA at physiologic conditions and even at ionic strengths above cellular levels. These results directly confirm the previous notion that the ASH1 transport machinery is not activated by the cargo RNA itself, but rather through protein-protein interactions.

  17. Models for microtubule cargo transport coupling the Langevin equation to stochastic stepping motor dynamics: Caring about fluctuations.

    Science.gov (United States)

    Bouzat, Sebastián

    2016-01-01

    One-dimensional models coupling a Langevin equation for the cargo position to stochastic stepping dynamics for the motors constitute a relevant framework for analyzing multiple-motor microtubule transport. In this work we explore the consistence of these models focusing on the effects of the thermal noise. We study how to define consistent stepping and detachment rates for the motors as functions of the local forces acting on them in such a way that the cargo velocity and run-time match previously specified functions of the external load, which are set on the base of experimental results. We show that due to the influence of the thermal fluctuations this is not a trivial problem, even for the single-motor case. As a solution, we propose a motor stepping dynamics which considers memory on the motor force. This model leads to better results for single-motor transport than the approaches previously considered in the literature. Moreover, it gives a much better prediction for the stall force of the two-motor case, highly compatible with the experimental findings. We also analyze the fast fluctuations of the cargo position and the influence of the viscosity, comparing the proposed model to the standard one, and we show how the differences on the single-motor dynamics propagate to the multiple motor situations. Finally, we find that the one-dimensional character of the models impede an appropriate description of the fast fluctuations of the cargo position at small loads. We show how this problem can be solved by considering two-dimensional models.

  18. Catalytic Propulsion and Magnetic Steering of Soft, Patchy Microcapsules: Ability to Pick-Up and Drop-Off Microscale Cargo.

    Science.gov (United States)

    Lu, Annie Xi; Liu, Yijing; Oh, Hyuntaek; Gargava, Ankit; Kendall, Eric; Nie, Zhihong; DeVoe, Don L; Raghavan, Srinivasa R

    2016-06-22

    We describe the creation of polymeric microcapsules that can exhibit autonomous motion along defined trajectories. The capsules are made by cross-linking aqueous microdroplets of the biopolymer chitosan using glutaraldehyde. A coflow microfluidic tubing device is used to generate chitosan droplets containing nanoparticles (NPs) with an iron (Fe) core and a platinum (Pt) shell. The droplets are then incubated in a Petri dish with the cross-linking solution, and an external magnet is placed below the Petri dish to pull the NPs together as a collective "patch" on one end of each droplet. This results in cross-linked capsules (∼150 μm in diameter) with an anisotropic (patchy) structure. When these capsules are placed in a solution of H2O2, the Pt shell of the NPs catalyzes the decomposition of H2O2 into O2 gas, which is ejected from the patchy end in the form of bubbles. As a result, the capsules (which are termed micromotors) move in a direction opposite to the bubbles. Furthermore, the micromotors can be steered along specific paths by an external magnet (the magnetic response arises due to the Fe in the core of the NPs). A given micromotor can thus be directed to meet with and adhere to an inert capsule, i.e., a model cargo. Adhesion occurs due to the soft nature of the two structures. Once the cargo is picked up, the micromotor-cargo pair can be moved along a specific path to a destination, whereupon the cargo can be released from the micromotor. We believe these soft micromotors offer significant benefits over their existing hard counterparts because of their biocompatibility, biodegradability, and ability to encapsulate a variety of payloads.

  19. 民机货舱压差载荷的分析与解决方案%Analysis and Solution of Civil Aircraft Cargo Differential Pressure Load

    Institute of Scientific and Technical Information of China (English)

    王旭阳; 林石泉; 李革萍

    2014-01-01

    One-dimension model is built up for cargo differential pressure calculation in this article, and then the effect of cargo volume and cargo leakage areas on cargo differential pressure load is analyzed. Secondly, this article brief introduces the internal design guide of cargo pressure equalization equipment. And the configurations of pressure equalization valves equipped on China self-developed civil aircraft are presented then. At last, a novel solution of cargo differential pressure load is proposed. The outlet of cargo ventilation shut-off valve is considered to keep open in normal condition to achieve differential pressure relief for cargo. This novel solution will bring some decrease of A/C weight and cost.%本文建立了一维的货舱压差载荷计算方法,并对货舱容积和货舱等效泄漏面积等因素对货舱压差载荷的影响进行了分析。介绍了我国民机货舱压差平衡装置的设计准则,简介了我国自主研制的货舱压差平衡装置结构。最后提出了一种新颖的货舱压差载荷解决方案,即把货舱压差平衡功能集成至货舱出口关断活门中。该方案可减少一定的飞机重量,并节省原货舱压力平衡装置的研制成本。

  20. The hemopexin and O-glycosylated domains tune gelatinase B/MMP-9 bioavailability via inhibition and binding to cargo receptors

    DEFF Research Database (Denmark)

    Van den Steen, Philippe E; Van Aelst, Ilse; Hvidberg, Vibeke;

    2006-01-01

    with a compact three-dimensional structure. The OG and hemopexin domains have no influence on the cleavage efficiency of MMP-9 substrates. In contrast, the hemopexin domain contains a binding site for the cargo receptor low density lipoprotein receptor-related protein-1 (LRP-1). Furthermore, megalin/LRP-2...... domains down-regulate the bioavailability of active MMP-9 and the interactions with the cargo receptors are proposed to be the original function of hemopexin domains in MMPs....

  1. Voltage-Responsive Controlled Release Film with Cargo Release Self-Monitoring Property Based on Hydrophobicity Switching.

    Science.gov (United States)

    Jiao, Xiangyu; Li, Yanan; Li, Fengyu; Sun, Ruijuan; Wang, Wenqian; Wen, Yongqiang; Song, Yanlin; Zhang, Xueji

    2017-03-16

    Herein, voltage-responsive controlled release film was constructed by grafting ferrocene on the mesoporous inverse opal photonic crystal (mIOPC). The film achieved free-blockage controlled release and realized the monitoring of cargo release without external indicator. Free-blockage was attributed to the voltage switchable nanovalves which undergo hydrophobic-to-hydrophilic transition when applying voltage. Monitoring of cargo release was attributed to the optical property of mIOPC, the bandgap of mIOPC had a red shift when the solution invaded in. The film was hydrophobic enough to stop solution intrusion. Once the voltage was applied, the film became hydrophilic, leading to invasion of the solution. As a result, the cargos were released and the bandgap of mIOPC was red-shifted. Therefore, in this paper both a free-blockage controlled release film and a release sensing system was prepared. The study provides new insights into highly effective controlled release and release sensing without indicator.

  2. National Security Science and Technology Initiative: Air Cargo Screening, Final Report for CRADA Number NFE-07-01081

    Energy Technology Data Exchange (ETDEWEB)

    Bingham, Philip [ORNL; Bush, John [Battelle Memorial Institute; Bowerman, Biays [Brookhaven National Laboratory; Cespedes, Ernesto [Idaho National Laboratory; White, Timothy [Pacific Northwest National Laboratory

    2004-12-01

    The non-intrusive inspection (NII) of consolidated air cargo carried on commercial passenger aircraft continues to be a technically challenging, high-priority requirement of the Department of Homeland Security’s Science and Technology Directorate (DHS S&T), the Transportation Security Agency and the Federal Aviation Administration. The goal of deploying a screening system that can reliably and cost-effectively detect explosive threats in consolidated cargo without adversely affecting the flow of commerce will require significant technical advances that will take years to develop. To address this critical National Security need, the Battelle Memorial Institute (Battelle), under a Cooperative Research and Development Agreement (CRADA) with four of its associated US Department of Energy (DOE) National Laboratories (Oak Ridge, Pacific Northwest, Idaho, and Brookhaven), conducted a research and development initiative focused on identifying, evaluating, and integrating technologies for screening consolidated air cargo for the presence of explosive threats. Battelle invested $8.5M of internal research and development funds during fiscal years 2007 through 2009.

  3. A Proposal for High-resolution X-ray Imaging of Intermodal Cargo Containers for Fissionable Materials

    Energy Technology Data Exchange (ETDEWEB)

    Quiter, B J; Prussin, S G; Pohl, B; Hall, J; Trebes, J; Stone, G; Descalle, M

    2007-07-05

    The sensitivity for identification of high-Z objects in elemental form in the massive cargo of intermodal containers with continuous bremsstrahlung radiation depends critically on discriminating the weak signal from uncollided photons from the very intense flux of scattered radiations that penetrate the cargo. We propose that this might be accomplished by rejection of detected events with E {le} 2-3 MeV that contain the majority of multiply-scattered photons along with a correction for single-scattered photons at higher energies. Monte Carlo simulations of radiographs with a 9-MeV bremsstrahlung spectrum demonstrate that rejection of detected events with E {le} 3 MeV removes the majority of signals from scattered photons emerging through cargo with Z {le} 30 and areal densities of at least 145 g cm{sup -2}. With analytical estimates of the single-scattered intensity at higher energies, accurate estimates of linear attenuation coefficients for shielded and unshielded uranium spheres with masses as small as 0.08 kg are found. The estimated maximum dose is generally so low that reasonable order tomography of interesting portions of a container should be possible.

  4. Survey of Fire Detection Technologies and System Evaluation/Certification Methodologies and Their Suitability for Aircraft Cargo Compartments

    Science.gov (United States)

    Cleary, T.; Grosshandler, W.

    1999-01-01

    As part of the National Aeronautics and Space Administration (NASA) initiated program on global civil aviation, NIST is assisting Federal Aviation Administration in its research to improve fire detection in aircraft cargo compartments. Aircraft cargo compartment detection certification methods have been reviewed. The Fire Emulator-Detector Evaluator (FE/DE) has been designed to evaluate fire detection technologies such as new sensors, multi-element detectors, and detectors that employ complex algorithms. The FE/DE is a flow tunnel that can reproduce velocity, temperature, smoke, and Combustion gas levels to which a detector might be exposed during a fire. A scientific literature survey and patent search have been conducted relating to existing and emerging fire detection technologies, and the potential use of new fire detection strategies in cargo compartment areas has been assessed. In the near term, improved detector signal processing and multi-sensor detectors based on combinations of smoke measurements, combustion gases and temperature are envisioned as significantly impacting detector system performance.

  5. CargoCBM - Feature Generation and Classification for a Condition Monitoring System for Freight Wagons

    Science.gov (United States)

    Gericke, C.; Hecht, M.

    2012-05-01

    Despite the fact that rail freight transport is one of the most environmentally friendly matters of transport, its growth has been far behind the growth of freight transport in general. Studies showed that a competitive disadvantage is caused by a low availability of rolling stock, especially freight wagons. Changing from a time based to a condition based maintenance strategy is believed to decrease down times by at least one third. To make condition based maintenance for freight wagons possible the TU Berlin and five industry partners started the research project CargoCBM. One task in this project is to develop algorithms for the automatic on-board diagnosis of wheel flats. The focus of the work is on the process of feature generation and feature selection as well as the application of different classifiers to automatically evaluate the data. Based on the results of measured data, features were selected and tested with different classifiers. Thought advanced classifiers such as neural networks have been analysed in accordance to their classification accuracy. It can be shown that with carefully constructed and selected features comparatively simple classifiers can lead to excellent results.

  6. Release of surfactant cargo from interfacially-active halloysite clay nanotubes for oil spill remediation.

    Science.gov (United States)

    Owoseni, Olasehinde; Nyankson, Emmanuel; Zhang, Yueheng; Adams, Samantha J; He, Jibao; McPherson, Gary L; Bose, Arijit; Gupta, Ram B; John, Vijay T

    2014-11-18

    Naturally occurring halloysite clay nanotubes are effective in stabilizing oil-in-water emulsions and can serve as interfacially-active vehicles for delivering oil spill treating agents. Halloysite nanotubes adsorb at the oil-water interface and stabilize oil-in-water emulsions that are stable for months. Cryo-scanning electron microscopy (Cryo-SEM) imaging of the oil-in-water emulsions shows that these nanotubes assemble in a side-on orientation at the oil-water interface and form networks on the interface through end-to-end linkages. For application in the treatment of marine oil spills, halloysite nanotubes were successfully loaded with surfactants and utilized as an interfacially-active vehicle for the delivery of surfactant cargo. The adsorption of surfactant molecules at the interface serves to lower the interfacial tension while the adsorption of particles provides a steric barrier to drop coalescence. Pendant drop tensiometry was used to characterize the dynamic reduction in interfacial tension resulting from the release of dioctyl sulfosuccinate sodium salt (DOSS) from halloysite nanotubes. At appropriate surfactant compositions and loadings in halloysite nanotubes, the crude oil-saline water interfacial tension is effectively lowered to levels appropriate for the dispersion of oil. This work indicates a novel concept of integrating particle stabilization of emulsions together with the release of chemical surfactants from the particles for the development of an alternative, cheaper, and environmentally-benign technology for oil spill remediation.

  7. Analysis of a Gross Counting Decision Metric for use in Threat Detection During Cargo Container Inspection

    Energy Technology Data Exchange (ETDEWEB)

    Hall, J

    2006-04-28

    LLNL is actively engaged in the development of a variety of advanced technologies for use in detecting potential threats in sea-going cargo containers, particularly the presence of hidden special nuclear materials (SNM). One such project is the so-called ''Nuclear Car Wash'' (NCW), which uses a high-energy neutron probe to scan the container. High-energy, {beta}-delayed {gamma}-rays emitted during the decay of short-lived, neutron-induced fission products are then taken as a signature of fissionable material. There are a number of different threat decision metrics that one could imagine using in conjunction with an inspection system such as the NCW; however, the most straightforward approach might be to simply compare the total number of counts that our detector records during some suitably chosen time interval to the average background signal that one would expect from a ''clean'' container during the same interval. The purpose of this report is to describe the basic statistical properties of a decision metric of this sort and outline the procedures for using it in experimental practice.

  8. Polymeric nanocapsules with up-converting nanocrystals cargo make ideal fluorescent bioprobes

    Science.gov (United States)

    Bazylińska, U.; Wawrzyńczyk, D.; Kulbacka, J.; Frąckowiak, R.; Cichy, B.; Bednarkiewicz, A.; Samoć, M.; Wilk, K. A.

    2016-07-01

    An innovative approach for up-converting nanoparticles adaptation for bio-related and theranostic applications is presented. We have successfully encapsulated multiple, ~8 nm in size NaYF4 nanoparticles inside the polymeric nanocarriers with average size of ~150 nm. The initial coating of nanoparticles surfaces was preserved due to the hydrophobic environment inside the nanocapsules, and thus no single nanoparticle surface functionalization was necessary. The selection of biodegradable and sugar-based polyelectrolyte shells ensured biocompatibility of the nanostructures, while the choice of Tm3+ and Yb3+ NaYF4 nanoparticles co-doping allowed for near-infrared to near-infrared bioimaging of healthy and cancerous cell lines. The protective role of organic shell resulted in not only preserved high up-converted emission intensity and long luminescence lifetimes, without quenching from water environment, but also ensured low cytotoxicity and high cellular uptake of the engineered nanocapsules. The multifunctionality of the proposed nanocarriers is a consequence of both the organic exterior part that is accessible for conjugation with biologically important molecules, and the hydrophobic interior, which in future application may be used as a container for co-encapsulation of inorganic nanoparticles and anticancer drug cargo.

  9. Detection of explosives, shielded nuclear materials and other hazardous substances in cargo containers

    Science.gov (United States)

    Kuznetsov, Andrey; Evsenin, Alexey; Vakhtin, Dmitry; Gorshkov, Igor; Osetrov, Oleg; Kalinin, Valery

    2006-05-01

    Nanosecond Neutron Analysis / Associated Particles Technique (NNA/APT) has been used to create devices for detection of explosives, radioactive and heavily shielded nuclear materials in cargo containers. Explosives and other hazardous materials are detected by analyzing secondary high-energy gamma-rays form reactions of fast neutrons with the materials inside the container. Depending on the dimensions of the inspected containers, the detecting system consists of one or several detection modules, each of which contains a small neutron generator with built-in position sensitive detector of associated alpha-particles and several scintillator-based gamma-ray detectors. The same gamma-ray detectors are used to detect unshielded radioactive and nuclear materials. Array of several detectors of fast neutrons is used to detect neutrons from spontaneous and induced fission of nuclear materials. These neutrons can penetrate thick layers of lead shielding, which can be used to conceal gamma-radioactivity from nuclear materials. Coincidence and timing analysis allows one to discriminate between fission neutrons and scattered probing neutrons. Mathematical modeling by MCNP5 code was used to estimate the sensitivity of the device and its optimal configuration. Capability of the device to detect 1 kg of explosive imitator inside container filled with suitcases and other baggage items has been confirmed experimentally. First experiments with heavily shielded nuclear materials have been carried out.

  10. Device for Detection of Explosives, Nuclear and Other Hazardous Materials in Luggage and Cargo Containers

    Science.gov (United States)

    Kuznetsov, Andrey; Evsenin, Alexey; Gorshkov, Igor; Osetrov, Oleg; Vakhtin, Dmitry

    2009-12-01

    Device for detection of explosives, radioactive and heavily shielded nuclear materials in luggage and cargo containers based on Nanosecond Neutron Analysis/Associated Particles Technique (NNA/APT) is under construction. Detection module consists of a small neutron generator with built-in position-sensitive detector of associated alpha-particles, and several scintillator-based gamma-ray detectors. Explosives and other hazardous chemicals are detected by analyzing secondary high-energy gamma-rays from reactions of fast neutrons with materials inside a container. The same gamma-ray detectors are used to detect unshielded radioactive and nuclear materials. An array of several neutron detectors is used to detect fast neutrons from induced fission of nuclear materials. Coincidence and timing analysis allows one to discriminate between fission neutrons and scattered probing neutrons. Mathematical modeling by MCNP5 and MCNP-PoliMi codes was used to estimate the sensitivity of the device and its optimal configuration. Comparison of the features of three gamma detector types—based on BGO, NaI and LaBr3 crystals is presented.

  11. Monte Carlo parametric studies of neutron interrogation with the Associated Particle Technique for cargo container inspections

    Science.gov (United States)

    Deyglun, Clément; Carasco, Cédric; Pérot, Bertrand

    2014-06-01

    The detection of Special Nuclear Materials (SNM) by neutron interrogation is extensively studied by Monte Carlo simulation at the Nuclear Measurement Laboratory of CEA Cadarache (French Alternative Energies and Atomic Energy Commission). The active inspection system is based on the Associated Particle Technique (APT). Fissions induced by tagged neutrons (i.e. correlated to an alpha particle in the DT neutron generator) in SNM produce high multiplicity coincidences which are detected with fast plastic scintillators. At least three particles are detected in a short time window following the alpha detection, whereas nonnuclear materials mainly produce single events, or pairs due to (n,2n) and (n,n'γ) reactions. To study the performances of an industrial cargo container inspection system, Monte Carlo simulations are performed with the MCNP-PoliMi transport code, which records for each neutron history the relevant information: reaction types, position and time of interactions, energy deposits, secondary particles, etc. The output files are post-processed with a specific tool developed with ROOT data analysis software. Particles not correlated with an alpha particle (random background), counting statistics, and time-energy resolutions of the data acquisition system are taken into account in the numerical model. Various matrix compositions, suspicious items, SNM shielding and positions inside the container, are simulated to assess the performances and limitations of an industrial system.

  12. Preliminary study of a large span-distributed-load flying-wing cargo airplane concept

    Science.gov (United States)

    Jernell, L. S.

    1978-01-01

    An aircraft capable of transporting containerized cargo over intercontinental distances is analyzed. The specifications for payload weight, density, and dimensions in essence configure the wing and establish unusually low values of wing loading and aspect ratio. The structural weight comprises only about 18 percent of the design maximum gross weight. Although the geometric aspect ratio is 4.53, the winglet effect of the wing-tip-mounted vertical tails, increase the effective aspect ratio to approximately 7.9. Sufficient control power to handle the large rolling moment of inertia dictates a relatively high minimum approach velocity of 315 km/hr (170 knots). The airplane has acceptable spiral, Dutch roll, and roll-damping modes. A hardened stability augmentation system is required. The most significant noise source is that of the airframe. However, for both take-off and approach, the levels are below the FAR-36 limit of 108 db. The design mission fuel efficiency is approximately 50 percent greater than that of the most advanced, currently operational, large freighter aircraft. The direct operating cost is significantly lower than that of current freighters, the advantage increasing as fuel price increases.

  13. Book Review: Radiation protection and measurement issues related to cargo scanning with accelerator-produced high-energy X rays, NCRP Commentary No. 20

    Energy Technology Data Exchange (ETDEWEB)

    Robert May

    2008-11-01

    Having spent roughly the first third of his health physics career on the Norfolk, VA waterfront area, the reviewer was excited to see the NCRP Commentary 20, 'Radiation Protection and Measurements Issues Related to Cargo Scanning with Accelerator Technology'. It signals the advent of the Cargo Advanced Automated Radiography System (CAARS). The waterfront is a border that challenges physical security programs and technology. As Commentary 20 provides in the introduction, waterfront cargo terminals and land border crossings together represent over 300 ports of entry in the USA. Every year, the USA receives over 10 million cargo containers from commercial shipping and a roughly equal amount from land border crossings. While rapidly processing containerized cargo, CAARS will be able to detect small quantities of high atomic number radioactive materials and dense shielding materials used for radioactive gamma ray sources and even illicit human cargo - important concerns for homeland security. It will also be able to detect other contraband such as explosives, weapons and drugs. Section 1 of the Commentary presents an executive summary with NCRP's radiation dose management recommendations and related operational recommendations for effective implementation of CAARS technology in the current regulatory environment.

  14. Bridging Small Molecules to Modified Bacterial Microparticles Using a Disulphide Linkage: MIS416 as a Cargo Delivery System.

    Directory of Open Access Journals (Sweden)

    Francesco Mainini

    Full Text Available MIS416 is an intact minimal cell wall skeleton derived from Proprionibacterium acnes that is phagocytosed by antigen presenting cells, including dendritic cells (DCs. This property allows MIS416 to be exploited as a vehicle for the delivery of peptide antigens or other molecules (for example, nucleic acids to DCs. We previously showed that covalent (non-cleavable conjugation of OVA, a model antigen derived from ovalbumin, to MIS416 enhanced immune responses in DCs in vivo, compared to unconjugated MIS416 and OVA. Intracellular trafficking promotes the lysosomal degradation of MIS416, leading to the destruction of MIS416 plus the associated cargos conjugated to MIS416. However, lysosomal degradation of cargo may not be desired for some MIS416 conjugates. Here we have investigated whether a cleavable linkage could facilitate release of the cargo in the cytoplasm of DCs to avoid lysosomal degradation. DCs were treated in vitro with disulfide-containing conjugates, and as hypothesised faster release of SIINFEKL peptide in the cytoplasm of DCs was observed with the inclusion of a disulfide bond between MIS416 and cargo. The inclusion of a cleavable disulfide bond in the conjugates did not significantly alter the amount of SIINFEKL antigens presented on MHC I molecules on DCs as compared with conjugates without a disulfide bond. However, the conjugates containing disulfide-linkages performed either slightly better (p<0.05 than, or the same as conjugates without a disulfide bond with respect to in vitro OT-1 T-cell proliferation induced by the presentation of SIINFEKL antigens on DCs, or DC activation studies, respectively. However, disulfide-containing conjugates were less effective than conjugates without a disulfide bond in in vivo cytotoxicity assays. In conclusion, inclusion of a disulfide bond in MIS416-peptide conjugates was associated with efficient release of peptides in the cytoplasm of DCs, an important consideration for MIS416-mediated

  15. The cargo protein MAP17 (PDZK1IP1) regulates the cancer stem cell pool activating the Notch pathway by abducting NUMB.

    Science.gov (United States)

    Garcia-Heredia, Jose M; Lucena-Cacace, Antonio; Verdugo-Sivianes, Eva M; Perez, Marco; Carnero, Amancio

    2017-02-02

    Purpose Cancer stem cells (CSCs) are self-renewing tumor cells, with ability to generate the diverse differentiated tumor cell subpopulations. They differ from normal stem cells in the deregulation of the mechanisms that normally controls stem cell physiology. CSCs are the origin of metastasis and highly resistant to therapy. Therefore the understanding of the CSC origin and deregulated pathways is important for tumor control. Experimental design We have included experiments in vitro, in cell lines and tumors of different origins. We have used PDXs and public transcriptomic databases of human tumors. Results MAP17 (PDZKIP1), a small cargo protein overexpressed in tumors, interacts with NUMB through the PDZ-binding domain activating the Notch pathway, leading to an increase in stem cell factors and cancer-initiating-like cells. Identical behavior was mimicked by inhibiting NUMB. Conversely, MAP17 downregulation in a tumor cell line constitutively expressing this gene led to Notch pathway inactivation and a marked reduction of stemness. In PDX models, MAP17 levels directly correlated with tumorsphere formation capability. Finally, in human colon, breast or lung there is a strong correlation of MAP17 expression with a signature of Notch and stem cell genes. Conclusions MAP17 overexpression activates Notch pathway by sequestering NUMB. High levels of MAP17 correlated with tumorspheres formation and Notch and Stem gene transcription. Its direct modification causes direct alteration of tumorsphere number and Notch and Stem pathway transcription. This defines a new mechanism of Notch pathway activation and Stem cell pool increase that may be active in a large percentage of tumors.

  16. DYNC1H1 mutations associated with neurological diseases compromise processivity of dynein-dynactin-cargo adaptor complexes.

    Science.gov (United States)

    Hoang, Ha Thi; Schlager, Max A; Carter, Andrew P; Bullock, Simon L

    2017-02-28

    Mutations in the human DYNC1H1 gene are associated with neurological diseases. DYNC1H1 encodes the heavy chain of cytoplasmic dynein-1, a 1.4-MDa motor complex that traffics organelles, vesicles, and macromolecules toward microtubule minus ends. The effects of the DYNC1H1 mutations on dynein motility, and consequently their links to neuropathology, are not understood. Here, we address this issue using a recombinant expression system for human dynein coupled to single-molecule resolution in vitro motility assays. We functionally characterize 14 DYNC1H1 mutations identified in humans diagnosed with malformations in cortical development (MCD) or spinal muscular atrophy with lower extremity predominance (SMALED), as well as three mutations that cause motor and sensory defects in mice. Two of the human mutations, R1962C and H3822P, strongly interfere with dynein's core mechanochemical properties. The remaining mutations selectively compromise the processive mode of dynein movement that is activated by binding to the accessory complex dynactin and the cargo adaptor Bicaudal-D2 (BICD2). Mutations with the strongest effects on dynein motility in vitro are associated with MCD. The vast majority of mutations do not affect binding of dynein to dynactin and BICD2 and are therefore expected to result in linkage of cargos to dynein-dynactin complexes that have defective long-range motility. This observation offers an explanation for the dominant effects of DYNC1H1 mutations in vivo. Collectively, our results suggest that compromised processivity of cargo-motor assemblies contributes to human neurological disease and provide insight into the influence of different regions of the heavy chain on dynein motility.

  17. 我国航空货运信息化发展现状与对策%The Status and Countermeasures of Development of Chinese Air Cargo Informatization

    Institute of Scientific and Technical Information of China (English)

    曾小林

    2015-01-01

    With the rapid development of the air cargo market. The role of the information technology becomes more and more important. This article points out the development status of our air cargo informatization by introducing the development of the world air cargo informatization. It comprehensively analyzes the main problems of the air cargo informatization in our country and puts forward countermeasures and suggestions to speed up the air cargo informatization construction in China on the basis of learning from foreign advanced experience and summing up the experience itself.%随着航空货运市场的快速发展,信息技术的作用越来越重要。本文通过介绍我国航空货运信息化的发展历程和现状,全面剖析了我国航空货运信息化存在的主要问题,在学习借鉴国外先进经验和总结自身经验教训的基础上,系统提出加快我国航空货运信息化建设的对策措施建议。

  18. The Caenorhabditis elegans Kinesin-3 motor UNC-104/KIF1A is degraded upon loss of specific binding to cargo.

    Directory of Open Access Journals (Sweden)

    Jitendra Kumar

    2010-11-01

    Full Text Available UNC-104/KIF1A is a Kinesin-3 motor that transports synaptic vesicles from the cell body towards the synapse by binding to PI(4,5P(2 through its PH domain. The fate of the motor upon reaching the synapse is not known. We found that wild-type UNC-104 is degraded at synaptic regions through the ubiquitin pathway and is not retrogradely transported back to the cell body. As a possible means to regulate the motor, we tested the effect of cargo binding on UNC-104 levels. The unc-104(e1265 allele carries a point mutation (D1497N in the PI(4,5P(2 binding pocket of the PH domain, resulting in greatly reduced preferential binding to PI(4,5P(2in vitro and presence of very few motors on pre-synaptic vesicles in vivo. unc-104(e1265 animals have poor locomotion irrespective of in vivo PI(4,5P(2 levels due to reduced anterograde transport. Moreover, they show highly reduced levels of UNC-104 in vivo. To confirm that loss of cargo binding specificity reduces motor levels, we isolated two intragenic suppressors with compensatory mutations within the PH domain. These show partial restoration of in vitro preferential PI(4,5P(2 binding and presence of more motors on pre-synaptic vesicles in vivo. These animals show improved locomotion dependent on in vivo PI(4,5P(2 levels, increased anterograde transport, and partial restoration of UNC-104 protein levels in vivo. For further proof, we mutated a conserved residue in one suppressor background. The PH domain in this triple mutant lacked in vitro PI(4,5P(2 binding specificity, and the animals again showed locomotory defects and reduced motor levels. All allelic variants show increased UNC-104 levels upon blocking the ubiquitin pathway. These data show that inability to bind cargo can target motors for degradation. In view of the observed degradation of the motor in synaptic regions, this further suggests that UNC-104 may get degraded at synapses upon release of cargo.

  19. Role of Air Cargo and Road Feeder Services for Regional Airports – Case Studies from the Baltic Sea Region

    Directory of Open Access Journals (Sweden)

    Beifert Anatoli

    2016-06-01

    Full Text Available Although airports play an important role the global and European economies, according to the new guidelines of the EU Commission on the new state aid rules for a competitive aviation industry (EC, 2014, it will be more difficult for unprofitable airports, to get financial public subsidies in the future. The Commission noted that the majority of regional airports experience severe problems to cover their operative costs, resulting from among other things from overlapping of airports’ catchment areas. The main objective of the above mentioned measures is not to close the regional airports, but to motivate them to operate on cost efficient, profitable and more flexible basis, stating however that and that the most inefficient airports will be still closed. In order face upcoming challenges, the regional airports shall reconsider their future development plans. Traditionally, the airports including small and regional airports focus on the passenger traffic and thus, the possible benefits and opportunities of the air cargo market are totally underestimated or even completely ignored. Most regional airports in the Baltic Sea region act totally isolated and do not have a clear picture of the current situation on the international air cargo market today, its future perspectives and sustainable development plans remain basically hidden. There are some examples when trying to meet the market demand, the regional airports have made huge, but unfortunately unjustified investments, e.g. improving airport infrastructure, runways, etc. However, the airports’ infrastructural investments alone cannot fulfil the expected “remedy” role for the airports’ sustainability. This paper aims at investigating the positioning and the role of the regional airports in the current hub-and-spoke network, focusing on the relevance and potential of the air cargo sector for the regional airports development. The optional freight value proposition for the development of

  20. Problemática Laboral de la Mujer en Cargos Directivos de los Servicios Públicos en Chile

    OpenAIRE

    Julio García del Junco; Adriana Riquelme

    2008-01-01

    A pesar de una mejor formación y participación en el mundo laboral, la mayoría de las mujeres, continúan enfrentadas al denominado "techo de cristal": la barrera invisible que les impide el acceso a los puestos de alta dirección en las organizaciones. Esta investigación analiza las distintas barreras o problemáticas laborales a las que se ven enfrentadas las mujeres directivas de los servicios públicos, ya sea para acceder y/o desempeñar dicho cargo. Para efectuar un análisis diferenciado, l...