WorldWideScience

Sample records for cargo off-loading mechanism

  1. Cargo-shell and cargo-cargo couplings govern the mechanics of artificially loaded virus-derived cages

    Science.gov (United States)

    Llauró, Aida; Luque, Daniel; Edwards, Ethan; Trus, Benes L.; Avera, John; Reguera, David; Douglas, Trevor; Pablo, Pedro J. De; Castón, José R.

    2016-04-01

    Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two types of proteinaceous payloads. While bound cargo to the inner capsid surface mechanically reinforced the capsid in a structural manner, unbound cargo diffusing freely within the shell cavity pressurized the cages up to ~30 atm due to steric effects. Strong cargo-cargo coupling reduces the resilience of these nanocompartments in ~20% when bound to the shell. Understanding the stability of artificially loaded nanocages will help to design more robust and durable molecular nanocontainers.Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two

  2. The interaction of equine lysozyme:oleic acid complexes with lipid membranes suggests a cargo off-loading mechanism

    DEFF Research Database (Denmark)

    Nielsen, Søren Bang; Wilhelm, Kristina; Vad, Brian;

    2010-01-01

    with oleic acids (ELOAs) were shown to possess tinctorial and morphological properties, similar to amyloidal aggregates, and to be cytotoxic. ELOA's interactions with phospholipid membranes appear to be central to its biological action, similar to human alpha-lactalbumin made lethal to tumor cells....... Here, we describe the interaction of ELOA with phospholipid membranes. Confocal scanning laser microscopy shows that ELOA, but not native EL, accumulates on the surface of giant unilamellar vesicles, without inducing significant membrane permeability. Quartz crystal microbalance with dissipation data...... indicated an essentially non-disruptive binding of ELOA to supported lipid bilayers, leading to formation of highly dissipative and "soft" lipid membrane; at higher concentrations of ELOA, the lipid membrane desorbs from the surface probably as bilayer sheets of vesicles. This membrane rearrangement...

  3. Cargo-shell and cargo-cargo couplings govern the mechanics of artificially loaded virus-derived cages.

    Science.gov (United States)

    Llauró, Aida; Luque, Daniel; Edwards, Ethan; Trus, Benes L; Avera, John; Reguera, David; Douglas, Trevor; Pablo, Pedro J de; Castón, José R

    2016-04-28

    Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two types of proteinaceous payloads. While bound cargo to the inner capsid surface mechanically reinforced the capsid in a structural manner, unbound cargo diffusing freely within the shell cavity pressurized the cages up to ∼30 atm due to steric effects. Strong cargo-cargo coupling reduces the resilience of these nanocompartments in ∼20% when bound to the shell. Understanding the stability of artificially loaded nanocages will help to design more robust and durable molecular nanocontainers. PMID:27091107

  4. SPECIFICS OF ORGANIZATIONAL AND FUNCTIONAL MECHANISM TO PROVIDE IMPLEMENTATION OF MARKETING STRATEGY OF CARGO HAULERS

    OpenAIRE

    Mitchenko, Galina

    2013-01-01

    Specifics of implementation of marketing strategy of cargo haulers are identified. Indicators of their marketing strategies’ efficiency are reviewed. The structure of organizational and functional mechanism of implementation of marketing strategies’ by cargo haulers is suggested.

  5. 29 CFR Appendix III to Part 1918 - The Mechanics of Conventional Cargo Gear (Non-mandatory)

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false The Mechanics of Conventional Cargo Gear (Non-mandatory.... 1918, App. III Appendix III to Part 1918—The Mechanics of Conventional Cargo Gear (Non-mandatory) Note: This appendix is non-mandatory and provides an explanation of the mechanics in the correct spotting...

  6. Crystal Structures of the Tetratricopeptide Repeat Domains of Kinesin Light Chains: Insight into Cargo Recognition Mechanisms

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Haizhong; Lee, Han Youl; Tong, Yufeng; Hong, Bum-Soo; Kim, Kyung-Phil; Shen, Yang; Lim, Kyung Jik; Mackenzie, Farrell; Tempel, Wolfram; Park, Hee-Won (SGC-Toronto); (PPCS); (Toronto)

    2012-10-23

    Kinesin-1 transports various cargos along the axon by interacting with the cargos through its light chain subunit. Kinesin light chains (KLC) utilize its tetratricopeptide repeat (TPR) domain to interact with over 10 different cargos. Despite a high sequence identity between their TPR domains (87%), KLC1 and KLC2 isoforms exhibit differential binding properties towards some cargos. We determined the structures of human KLC1 and KLC2 tetratricopeptide repeat (TPR) domains using X-ray crystallography and investigated the different mechanisms by which KLCs interact with their cargos. Using isothermal titration calorimetry, we attributed the specific interaction between KLC1 and JNK-interacting protein 1 (JIP1) cargo to residue N343 in the fourth TRP repeat. Structurally, the N343 residue is adjacent to other asparagines and lysines, creating a positively charged polar patch within the groove of the TPR domain. Whereas, KLC2 with the corresponding residue S328 did not interact with JIP1. Based on these finding, we propose that N343 of KLC1 can form 'a carboxylate clamp' with its neighboring asparagine to interact with JIP1, similar to that of HSP70/HSP90 organizing protein-1's (HOP1) interaction with heat shock proteins. For the binding of cargos shared by KLC1 and KLC2, we propose a different site located within the groove but not involving N343. We further propose a third binding site on KLC1 which involves a stretch of polar residues along the inter-TPR loops that may form a network of hydrogen bonds to JIP3 and JIP4. Together, these results provide structural insights into possible mechanisms of interaction between KLC TPR domains and various cargo proteins.

  7. Mechanized azobenzene-functionalized zirconium metal-organic framework for on-command cargo release

    Science.gov (United States)

    Meng, Xiangshi; Gui, Bo; Yuan, Daqiang; Zeller, Matthias; Wang, Cheng

    2016-01-01

    Stimuli-responsive metal-organic frameworks (MOFs) have gained increasing attention recently for their potential applications in many areas. We report the design and synthesis of a water-stable zirconium MOF (Zr-MOF) that bears photoresponsive azobenzene groups. This particular MOF can be used as a reservoir for storage of cargo in water, and the cargo-loaded MOF can be further capped to construct a mechanized MOF through the binding of β-cyclodextrin with the azobenzene stalks on the MOF surface. The resulting mechanized MOF has shown on-command cargo release triggered by ultraviolet irradiation or addition of competitive agents without premature release. This study represents a simple approach to the construction of stimuli-responsive mechanized MOFs, and considering mechanized UiO-68-azo made from biocompatible components, this smart system may provide a unique MOF platform for on-command drug delivery in the future. PMID:27493996

  8. A non-canonical mechanism for Crm1-export cargo complex assembly.

    Science.gov (United States)

    Fischer, Ute; Schäuble, Nico; Schütz, Sabina; Altvater, Martin; Chang, Yiming; Faza, Marius Boulos; Panse, Vikram Govind

    2015-01-01

    The transport receptor Crm1 mediates the export of diverse cargos containing leucine-rich nuclear export signals (NESs) through complex formation with RanGTP. To ensure efficient cargo release in the cytoplasm, NESs have evolved to display low affinity for Crm1. However, mechanisms that overcome low affinity to assemble Crm1-export complexes in the nucleus remain poorly understood. In this study, we reveal a new type of RanGTP-binding protein, Slx9, which facilitates Crm1 recruitment to the 40S pre-ribosome-associated NES-containing adaptor Rio2. In vitro, Slx9 binds Rio2 and RanGTP, forming a complex. This complex directly loads Crm1, unveiling a non-canonical stepwise mechanism to assemble a Crm1-export complex. A mutation in Slx9 that impairs Crm1-export complex assembly inhibits 40S pre-ribosome export. Thus, Slx9 functions as a scaffold to optimally present RanGTP and the NES to Crm1, therefore, triggering 40S pre-ribosome export. This mechanism could represent one solution to the paradox of weak binding events underlying rapid Crm1-mediated export. PMID:25895666

  9. An apoA-I mimetic peptide facilitates off-loading cholesterol from HDL to liver cells through scavenger receptor BI

    Directory of Open Access Journals (Sweden)

    Xuelei Song, Paul Fischer, Xun Chen, Charlotte Burton, Jun Wang

    2009-01-01

    Full Text Available Apolipoprotein A-I (apoA-I mimetic peptides have been pursued as new therapeutic agents for the treatment of atherosclerosis, yet their precise mechanism responsible for atheroprotection remains unclear. Like apoA-I itself, most of these peptides are capable of stimulating cholesterol efflux from macrophages or foam cells, and some of them stimulate lecithin cholesterol acyltransferase (LCAT activity in the reverse cholesterol transport (RCT pathway. However, the ability of mimetic peptides to deliver cholesterol into hepatocytes (off-loading, the last step of the RCT pathway, has not been demonstrated. In this study, we compared a mimetic peptide D-4F to purified apoA-I, to address the role that mimetics play during the off-loading process. Both D-4F and apoA-I formed spherical nano-particles when reconstituted with cholesteryl ester and phospholipids. Compared to apoA-I, D-4F particles were 20 times more efficient in off-loading cholesterol to HepG2 hepatocytes with an apparent Kt (transport of 0.74 μg/mL. Furthermore, D-4F also facilitated cholesteryl ester offloading from HDL particles into HepG2 cells when it was pre-incubated with these HDL particles. Using an inducible HEK293 cell line, we demonstrated that these nano-particles were able to be taken up through SR-BI, a HDL selective receptor. Cholesterol uptake by HepG2 cells was completely blocked by a neutralizing monoclonal antibody against SR-BI, demonstrating that D-4F particles, similar to HDL, specifically off-loaded cholesterol through SR-BI. Overall our data provides evidence that D-4F is capable of mimicking apoA-I to form HDL-like particles, and off-loads cholesterol for catabolism and excretion, thus completing RCT.

  10. Duration of off-loading and recurrence rate in Charcot osteo-arthropathy treated with less restrictive regimen with removable walker

    DEFF Research Database (Denmark)

    Christensen, Tomas M; Gade-Rasmussen, Birthe; Pedersen, Lis W;

    2013-01-01

    Recent literature on acute diabetic Charcot osteoarthropathy (CA) reports unusually long periods of off-loading. Data suggest that this might increase the re-currence rate. Subsequently we evaluated the influence of duration of off-loading on the risk of required re-casting.......Recent literature on acute diabetic Charcot osteoarthropathy (CA) reports unusually long periods of off-loading. Data suggest that this might increase the re-currence rate. Subsequently we evaluated the influence of duration of off-loading on the risk of required re-casting....

  11. 46 CFR 154.534 - Cargo pumps and cargo compressors.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pumps and cargo compressors. 154.534 Section 154... Equipment Cargo and Process Piping Systems § 154.534 Cargo pumps and cargo compressors. Cargo pumps and cargo compressors must shut-down automatically when the quick-closing shut-off valves under §§...

  12. Connection, Translation, Off-Loading, and Monitoring: A Framework for Characterizing the Pedagogical Functions of Educational Technologies

    Science.gov (United States)

    Martin, Lee

    2012-01-01

    Distributed cognition offers powerful tools for conceptualizing the role that technology plays in learning environments, yet it can be challenging to apply. This paper presents an analytical framework that focuses on four pedagogical functions that technology can perform in learning environments: connection, translation, off-loading, and…

  13. Perihelion Power Constraint and Wheel-Off-Loading Impact on BepiColombo's Relativity Experiment

    Science.gov (United States)

    Schuster, Anja Katharina; Jehn, Rüdiger

    2016-04-01

    In this paper the consequences of the Perihelion Power Constraint (PPC) are presented along with the impact of the Momentum Wheel-Off-loading (WOL) events on the estimation precision of the post-Newtonian parameters (PNP). This analysis will provide an estimate of the outcome of the relativity experiment in the framework of the joint mission between ESA and JAXA, named BepiColombo. The Mercury Planetary Orbiter (MPO), one of the two scientific elements of the composite spacecraft, aims to verify Einstein's theory of General Relativity plus alternative theories of gravitation to an unprecedented degree of accuracy [1]. The PPC describes the switch-off of the Ka-transponder when Mercury is ±35° around perihelion [2]. This causes a decrease in the quality of radiometric measurements. The implemented PPC distinguishes between different geometries such as inferior and superior constellations. Moreover, it differentiates between the case in which Mercury is close to superior solar conjunction (SSC) while being around perihelion and the one of only being in SSC. Our treatment has the essential effect that the standard deviation of range and doppler measurements is increased in the perihelion region by up to 1.83 ṡ 10‑5 km/s (doppler noise). There is no clear trend to be seen in the resulting PNP uncertainties. For instance, the value of the estimation bias of the PNP γ of 8.232 ṡ 10‑6 is almost by factor two larger than in the case when the PPC is not activated (4.243 ṡ 10‑6). But applying the PPC to the back-up trajectory this trend is not confirmed, since the reverse is true for the γ uncertainty σ(γPPC) special importance to humankind's exploration of space. One fundamental step is the experimental verification of theories. This analysis gives an overview of the sensitivity of the relativity experiment to the PPC and WOL. References [1] Milani, A., Vokrouhlický, D., Bonnano, C., & Rossi, A. (2002). Testing general relativity with the Bepi

  14. 46 CFR 154.1210 - Hold space, void space, cofferdam, and spaces containing cargo piping.

    Science.gov (United States)

    2010-10-01

    ... Design, Construction and Equipment Cargo Area: Mechanical Ventilation System § 154.1210 Hold space, void... spaces containing cargo piping must have: (1) A fixed mechanical ventilation system; or (2) A...

  15. Detailed description of an SSAC at the facility level for light water moderated (off-load refueled) power reactor facilities

    International Nuclear Information System (INIS)

    This report is intended to provide the technical details of an effective State Systems of Accounting for and Control of Nuclear Material (SSAC) which Member States may use, if they wish, to establish and maintain their SSACs. It is expected that systems designed along the lines described would be effective in meeting the objectives of both national and international systems for nuclear material accounting and control. This document accordingly provides a detailed description of a system for the accounting for and control of nuclear material in an off-load refueled light water moderated power reactor facility which can be used by a facility operator to establish his own system to comply with a national system for nuclear material accounting and control and to facilitate application of IAEA safeguards. The scope of this document is limited to descriptions of the following elements: (1) Nuclear Material Measurements; (2) Measurement Quality; (3) Records and Reports; (4) Physical Inventory Taking; (5) Material Balance Closing

  16. Groupage Cargo Transportation Model

    OpenAIRE

    Aleksejevs Ruslans; Guseinovs Raufs; Medvedev Alexander N.; Guseynov Sharif E.

    2016-01-01

    In this work we consider a specific problem of optimal planning of maritime transportation of multiproduct cargo by ships of one (corporate strategy) or several (partially corporate strategy) companies: the core of the problem consists of the existence of the network of intermediate seaports (i.e. transitional seaports), where for every ship arrived the cargo handling is done, and which are situated between the starting and the finishing seaports. In this work, there are mathematical models b...

  17. Environmental assessment for DOE permission for the off-loading and transportation of commercial low-level radioactive waste across the Savannah River Site

    International Nuclear Information System (INIS)

    The Department of Energy (DOE) prepared this Environmental Assessment (EA) to assess the potential environmental impacts associated with DOE allowing Chem-Nuclear Systems, L.L.C. (CNS) to off-load and transport low-level radioactive waste (LLW) packages across the Savannah River Site (SRS), located near Aiken, South Carolina, to the nearby CNS facility. The proposed action entails DOE granting permission to CNS to use SRS for landing shipping barges at the existing SRS boat ramp and off-loading trailered LLW packages for transportation across SRS to the CNS facility. Project activities would include modification of the SRS boat ramp on the Savannah River, as needed for off-loading activities, and construction of a bridge across Lower Three Runs. The proposed action also encompasses any similar future off-loading and transportation activities for LLW en route to the CNS facility. The National Environmental Policy Act requires the assessment of environmental consequences of Federal actions that may affect the quality of the human environment. Based on the potential for impacts described herein, DOE will either publish a Finding of No Significant Impact or prepare an Environmental Impact Statement (EIS)

  18. Groupage Cargo Transportation Model

    Directory of Open Access Journals (Sweden)

    Aleksejevs Ruslans

    2016-03-01

    Full Text Available In this work we consider a specific problem of optimal planning of maritime transportation of multiproduct cargo by ships of one (corporate strategy or several (partially corporate strategy companies: the core of the problem consists of the existence of the network of intermediate seaports (i.e. transitional seaports, where for every ship arrived the cargo handling is done, and which are situated between the starting and the finishing seaports. In this work, there are mathematical models built from scratch in the form of multicriteria optimization problem; then the goal attainment method of Gembicki is used for reducing the built models to a one-criterion problem of linear programming.

  19. Contingency strategy for insufficient full core off load capability in spent fuel pool for Chinshan nuclear power station

    International Nuclear Information System (INIS)

    The spent fuel pool (SFP) at Taiwan Power Company's (TUC's) Chinshan plant lost the full core off load (FCO) capability in 2010, even with the second SFP repacking project to expand the capacity as reported in 12PBNC. The TEPC had originally planned to move some spent fuel assemblies from SFP to dry storage facility, however, the dry storage project had seriously fell behind. Thus, it is required to address insufficient FCO capability, and the following contingency measures have been employed: The first step was to explore whether there was a specific regulatory requirement for FCO capability, and none were identified. Also, the industrial experiences were explored. The refueling strategy is changed from FCO to in-core shuffling. A feasibility evaluation performed indicates the Technical Specifications require: alternate method of decay heat removal, and verification of shutdown margin for each in vessel fuel movement. Specific methods have been successfully established. A safety evaluation for operation without FCO capability was performed, and no safety concerns were identified. The risk for operation without FCO capability was assessed. The previous operational experiences were identified. Moreover, such works are not expected in subsequent cycles. The new fuel vault is used to store new fuel assemblies. The criticality analysis has been performed and some new approaches are proposed to enhance the storage flexibility as reported in 17PBNC. An inter-unit transfer cask has been designed to transfer spent fuel from the SFP of one unit to the other. The FCO capability can be effectively extended for three more years with this consideration. The TPC discussed the contingency strategy with the ROCAEC in May 2006, and the ROCAEC's concurrence was attained. With the proposed strategy, Chinshan units have been operating smoothly

  20. 46 CFR 154.315 - Cargo pump and cargo compressor rooms.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pump and cargo compressor rooms. 154.315 Section... Equipment Ship Arrangements § 154.315 Cargo pump and cargo compressor rooms. (a) Cargo pump rooms and cargo compressor rooms must be above the weather deck and must be within the cargo area. (b) Where pumps...

  1. Bidirectionality From Cargo Thermal Fluctuations in Motor-Mediated Transport

    CERN Document Server

    Miles, Christopher E

    2016-01-01

    Molecular motor proteins serve as an essential component of intracellular transport by generating forces to haul cargoes along cytoskeletal filaments. In some circumstances, two species of motors that are directed oppositely (e.g. kinesin, dynein) can be attached to the same cargo. The resulting net motion is known to be bidirectional, but the mechanism of switching remains unclear. In this work, we propose a mean-field mathematical model of the mechanical interactions of two populations of molecular motors with diffusion of the cargo (thermal fluctuations) as the fundamental noise source. By studying a simplified model, the delayed response of motors to rapid fluctuations in the cargo is quantified, allowing for the reduction of the full model to two "characteristic positions" of each of the motor populations. The system is then found to be "metastable", switching between two distinct directional transport states, or bidirectional motion. The time to switch between these states is then investigated using WKB...

  2. Cargo-cult training

    Science.gov (United States)

    Magueijo, João

    2009-12-01

    Richard Feynman, in one of his famous rants, evoked as a metaphor what he called "cargo-cult science". During the Second World War, the indigenous people of the South Pacific became accustomed to US Air Force planes landing on their islands, invariably bringing a profusion of desirable goods and tasty foods. When the war ended, they were distressed by the discontinuation of this popular service. So, they decided to take action. They cleared elongated patches of land to make them look like runways. They lit wood fires where they had seen electric floodlights guiding in the planes. They built a wooden shack and made a man sit inside with two halves of a coconut on each ear and bamboo bars sticking out like antennas: he was the "air controller". And they waited for the planes to return.

  3. Working out of generalized dynamic model of cargo fixation with cargo pads jointly with flexible and thrust elements under the action spatial force system

    OpenAIRE

    Khabibulla TURANOV; Elena TIMUKHINA

    2011-01-01

    The article gives an account of the results of working out a generalized dynamic model of fixation of cargo with pads jointly with flexible and thrust elements. Working out a generalized dynamic model of cargo fixation is rather a complicated problem not in the sense of solving differential equations systems but in the sense of presenting them as a complex mechanical system “cargo – pads – flexible elements – thrust bars”. Generalized dynamic model of cargo with pads observed that this model ...

  4. Bidirectional cargo transport: Moving beyond tug-of-war

    Science.gov (United States)

    Hancock, William O.

    2016-01-01

    Preface Vesicles, organelles and other intracellular cargo are transported by kinesin and dynein motors, which move in opposite directions along microtubules. This bidirectional cargo movement is frequently described as a “tug-of-war” between oppositely-directed motors attached to the same cargo. However, although many experimental and modeling studies support the tug-of-war paradigm, numerous knockout and inhibition studies in a variety of systems have found that inhibiting one motor leads to diminished motility in both directions, which is a “paradox of codependence” that challenges it. In an effort to resolve this paradox, three classes of bidirectional transport models, termed microtubule tethering, mechanical activation, and steric disinhibition, are proposed and a general mathematical modeling framework for bidirectional cargo transport is put forward to guide future experiments. PMID:25118718

  5. Operational efficiency in STS cargo processing

    Science.gov (United States)

    Wilson, R. E.

    1985-01-01

    A multifaceted program is presented that addresses both the operational aspects of Shuttle-cargo integration and the needs of the STS Cargo Community. The program consists of the following key elements: (1) processing team awareness of cargo needs and requirements; (2) standardization of Orbiter preparation and cargo integration procedures and methods; (3) maximum application of state-of-the-art ADP techniques in all relevant areas; (4) continual review of cargo integration facility and ground system capabilities versus requirements and enhancement; (5) continual assessment of proposed cargo processing changes for safety and other needs; and (6) review of cargo processing philosophies, policies, and concepts for potential improvements.

  6. Clinical Efficacy and Safety of a New Method for Pressure Off-load for Patients with Diabetic Foot Syndrome: Ankle-foot Pneumoorthosis with TM Orlett

    Directory of Open Access Journals (Sweden)

    Galina Yurevna Strakhova

    2014-09-01

    Full Text Available AimThe purpose of this study was to assess the clinical efficacy, safety and consumer properties of ankle-foot pneumoorthosis with a HAS-337 TM Orlett compared with non-removable total contact cast (TCC immobilization.Materials and methodsOur study included 40 patients with diabetes mellitus type 1 (DM1 and type 2 (DM2 with neuropathic diabetic foot syndrome and chronic uninfected wounds of the plantar surface of the forefoot, with wound duration of at least 3 weeks, wound areas not less than 1 cm2 and wound depths not more than stage II based on Wagner’s classification. We excluded patients with infected wounds, osteomyelitis, Charcot osteoarthropathy or peripheral vascular disease. Our test group included 20 patients who received pressure off-load using ankle-foot pneumoorthosis with a HAS-337 TM Orlett. For a control group (n = 20, pressure off-load was achieved using TCC immobilization. Both groups were comparable with regard to age, gender, duration and degree of diabetes compensation and by original wound defect sizes (p >0.05. The study duration was 6 months. Plantar pressure was measured inside the orthosis or TCC and was compared with test shoe measurements. Our major criteria for pressure relief were reduced pressures in the wound area and the whole foot and the rate of wound healing.ResultsAt the end of the 6-month period, complete healing of all ulcers was achieved. The average healing time was 46.1±19.0 days for the test group and was 48.3±20.5 days for the control group (p >0.05. Two patients who wore pneumoorthosis with HAS-337 were discontinued upon patient request.With pneumoorthosis, the maximum peak pressure on the foot and wound defect areas was reduced by 26% and 57%, respectively. The pressure/time integral decreased on average by 41% (p >0.05. Furthermore, in the midfoot area with pneumoorthosis, the maximum pressure increased by 48% and the pressure/time integral increased by 47%.ConclusionsUsing pneumoorthosis with

  7. 46 CFR 105.25-7 - Ventilation systems for cargo tank or pumping system compartment.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Ventilation systems for cargo tank or pumping system compartment. 105.25-7 Section 105.25-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO... pumping system compartment. (a) Each compartment shall be provided with a mechanical exhaust...

  8. 49 CFR 1544.228 - Access to cargo and cargo screening: Security threat assessments for cargo personnel in the...

    Science.gov (United States)

    2010-10-01

    ... threat assessments for cargo personnel in the United States. 1544.228 Section 1544.228 Transportation... COMMERCIAL OPERATORS Operations § 1544.228 Access to cargo and cargo screening: Security threat assessments... paragraph (b) of this section— (1) Each individual must successfully complete a security threat...

  9. 46 CFR 153.908 - Cargo viscosity and melting point information; measuring cargo temperature during discharge...

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo viscosity and melting point information; measuring... Cargo viscosity and melting point information; measuring cargo temperature during discharge: Categories... lading, a written statement of the following: (1) For Category A or B NLS, the cargo's viscosity at 20...

  10. 46 CFR 154.1810 - Cargo manual.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo manual. 154.1810 Section 154.1810 Shipping COAST... SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Operations § 154.1810 Cargo manual. (a) No person... navigable waters of the United States, or a U.S. flag vessel, unless the vessel has on board a cargo...

  11. 46 CFR 153.907 - Cargo information.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo information. 153.907 Section 153.907 Shipping... Information § 153.907 Cargo information. (a) The master shall ensure that the following information for each... process for the vessel. (b) The master shall make sure that the following information for cargoes...

  12. Determination of high-risk cargo

    Science.gov (United States)

    Morris, Leo A.; Smith, Douglas E.; Khan, Siraj M.

    1994-10-01

    The approach and methodology used in the determination of the type of cargo containing concealments of commercial quantities of narcotics such as cocaine and heroin is described. This high-risk cargo enters the United States through border crossings at land, seaports and airports. The volume and variety of cargos make it a complex and challenging task for the U.S. Customs Service.

  13. Shift designs for freight handling personnel at air cargo terminals

    DEFF Research Database (Denmark)

    Rong, Aiying; Grunow, Martin

    2009-01-01

    resources efficiently, we implement a new mechanism for demand leveling. In addition, we consider the qualification hierarchy between build-up and break-down workers. A case study based on the real-life data shows that the model is useful for manpower planning at air cargo terminals and the integrated...

  14. Cargo Assured Access to International Space Station

    Science.gov (United States)

    Smith, David A.

    2004-01-01

    Boeing's Cargo Assured Access logistics delivery system will provide a means to transport cargo to/from the International Space Station, Low Earth Orbit and the moon using Expendable Launch Vehicles. For Space Station, this capability will reduce cargo resupply backlog during nominal operations (e.g., supplement Shuttle, Progress, ATV and HTV) and augment cargo resupply capability during contingency operations (e.g., Shuttle delay and/or unavailability of International Partner launch or transfer vehicles). This capability can also provide an autonomous means to deliver cargo to lunar orbit, a lunar orbit refueling and work platform, and a contingency crew safe haven in support of NASA's new Exploration Initiative.

  15. Spatial partitioning of secretory cargo from Golgi resident proteins in live cells

    Directory of Open Access Journals (Sweden)

    White Jamie

    2001-10-01

    Full Text Available Abstract Background To maintain organelle integrity, resident proteins must segregate from itinerant cargo during secretory transport. However, Golgi resident enzymes must have intimate access to secretory cargo in order to carry out glycosylation reactions. The amount of cargo and associated membrane may be significant compared to the amount of Golgi membrane and resident protein, but upon Golgi exit, cargo and resident are efficiently sorted. How this occurs in live cells is not known. Results We observed partitioning of the fluorescent Golgi resident T2-CFP and fluorescent cargo proteins VSVG3-YFP or VSVG3-SP-YFP upon Golgi exit after a synchronous pulse of cargo was released from the ER. Golgi elements remained stable in overall size, shape and relative position as cargo emptied. Cargo segregated from resident rapidly by blebbing into micron-sized domains that contained little or no detectable resident protein and that appeared to be continuous with the parent Golgi element. Post-Golgi transport carriers (TCs exited repeatedly from these domains. Alternatively, entire cargo domains exited Golgi elements, forming large TCs that fused directly with the plasma membrane. However, domain formation did not appear to be an absolute prerequisite for TC exit, since TCs also exited directly from Golgi elements in the absence of large domains. Quantitative cargo-specific photobleaching experiments revealed transfer of cargo between Golgi regions, but no discrete intra-Golgi TCs were observed. Conclusions Our results establish domain formation via rapid lateral partitioning as a general cellular strategy for segregating different transmembrane proteins along the secretory pathway and provide a framework for consideration of molecular mechanisms of secretory transport.

  16. Green channel cargo inspection system

    International Nuclear Information System (INIS)

    A radiation detection device was installed in the lanes of a highway toll station, radioactive rays which was collimated emitted through the measured, and arrived the detector. The average density of the fresh agricultural products belonged to Green channel and other prohibited items vary greatly, the absorption of radiation are different between the Green Channel Cargo and other substances. Prior to the experimental group, different standard samples which represent different models and goods were measured, the different standard samples were stored in a computer database. When the trucks get through the Green Channel, the detector will detect the radiation signal and bring to the computer, the computer will process the measured data, and make a conclusion whether the goods are Green Channel cargo. (authors)

  17. Structural Basis of Cargo Recognition by Unconventional Myosins in Cellular Trafficking.

    Science.gov (United States)

    Li, Jianchao; Lu, Qing; Zhang, Mingjie

    2016-08-01

    Unconventional myosins are a superfamily of actin-based molecular motors playing diverse roles including cellular trafficking, mechanical supports, force sensing and transmission, etc. The variable neck and tail domains of unconventional myosins function to bind to specific cargoes including proteins and lipid vesicles and thus are largely responsible for the diverse cellular functions of myosins in vivo. In addition, the tail regions, together with their cognate cargoes, can regulate activities of the motor heads. This review outlines the advances made in recent years on cargo recognition and cargo binding-induced regulation of the activity of several unconventional myosins including myosin-I, V, VI and X in cellular trafficking. We approach this topic by describing a series of high-resolution structures of the neck and tail domains of these unconventional myosins either alone or in complex with their specific cargoes, and by discussing potential implications of these structural studies on cellular trafficking of these myosin motors. PMID:26842936

  18. NWA Cargo Is Granted Additional Cargo Frequencies to China Four all-cargo frequencies per week granted by U.S. Department of Transportation

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ NWA Cargo, the cargo division of Northwest Airlines, will expand its all-cargo freighter service between the United States and China after being awarded four additional frequencies per week by the U.S. Department of Transportation.

  19. 46 CFR 64.89 - Cargo pump unit.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Cargo pump unit. 64.89 Section 64.89 Shipping COAST... HANDLING SYSTEMS Cargo Handling System § 64.89 Cargo pump unit. (a) A cargo pump unit that fills or...) The cargo pump power unit must be— (1) Diesel; (2) Hydraulic; (3) Pneumatic; or (4) Electric. (c)...

  20. Cargo-Positioning System for Next-Generation Spacecraft

    Science.gov (United States)

    Holladay, Jon; Colton, Jonathan

    2006-01-01

    A report discusses a proposed system for mounting loaded pallets in the cargo bay of a next-generation space-shuttle-like spacecraft, such that the center of mass of the cargo would lie within a 1-in. (2.54-cm) cube that would also contain the center of mass of the spacecraft. The system would include (1) an algorithm for planning the locations of the pallets, given the geometric and weight properties of the pallets, and the geometric restrictions of the cargo bay; (2) quick-connect/quick-disconnect mounting mechanisms similar to those now used on air hoses; (3) other mounting mechanisms, comprising mostly spring-loaded pins, in a locking subsystem that would prevent shifting of the pallets under load; and (4) mechanisms for performing fine position adjustments to satisfy the center-of-mass requirement. The position- adjusting mechanisms would be motor-driven lead-screw mechanisms in groups of three - one for positioning each pin of the locking subsystem along each of three mutually perpendicular coordinate axes. The system also would include a triple-threaded screw that would provide compensation for thermal expansion or contraction of the spacecraft.

  1. Study on Alternative Cargo Launch Options from the Lunar Surface

    Energy Technology Data Exchange (ETDEWEB)

    Cheryl A. Blomberg; Zamir A. Zulkefli; Spencer W. Rich; Steven D. Howe

    2013-07-01

    In the future, there will be a need for constant cargo launches from Earth to Mars in order to build, and then sustain, a Martian base. Currently, chemical rockets are used for space launches. These are expensive and heavy due to the amount of necessary propellant. Nuclear thermal rockets (NTRs) are the next step in rocket design. Another alternative is to create a launcher on the lunar surface that uses magnetic levitation to launch cargo to Mars in order to minimize the amount of necessary propellant per mission. This paper investigates using nuclear power for six different cargo launching alternatives, as well as the orbital mechanics involved in launching cargo to a Martian base from the moon. Each alternative is compared to the other alternative launchers, as well as compared to using an NTR instead. This comparison is done on the basis of mass that must be shipped from Earth, the amount of necessary propellant, and the number of equivalent NTR launches. Of the options, a lunar coil launcher had a ship mass that is 12.7% less than the next best option and 17 NTR equivalent launches, making it the best of the presented six options.

  2. 46 CFR 151.45-6 - Maximum amount of cargo.

    Science.gov (United States)

    2010-10-01

    ... temperature corresponding to the vapor pressure of the cargo at the safety relief valve setting. A reduction... required for a cargo vapor pressure equal to the relief valve setting. d L=Density of cargo at the...

  3. 46 CFR 28.885 - Cargo gear.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo gear. 28.885 Section 28.885 Shipping COAST GUARD... Aleutian Trade Act Vessels § 28.885 Cargo gear. (a) The safe working load (SWL) for the assembled gear... the load the gear is approved to lift, excluding the weight of the gear itself. (b) All wire...

  4. 48 CFR 470.203 - Cargo preference.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Cargo preference. 470.203 Section 470.203 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE FOOD ASSISTANCE PROGRAMS COMMODITY ACQUISITIONS 470.203 Cargo preference. An agency having responsibility under this subpart...

  5. National Security Science and Technology Initiative: Air Cargo Screening

    Energy Technology Data Exchange (ETDEWEB)

    Bingham, Philip R [ORNL; White, Tim [Pacific Northwest National Laboratory (PNNL); Cespedes, Ernesto [Idaho National Laboratory (INL); Bowerman, Biays [Brookhaven National Laboratory (BNL); Bush, John [Battelle

    2010-11-01

    explosive benchmarks combinations, and conducted modeling and simulation studies to begin populating the matrix of commodities, threats, and detection technologies. (4) Designed and fabricated basic (homogeneous) commodity test pallets and fabricated inert stimulants to support experiments and to validate modeling/simulation results. (5) Developed/expanded the team's capabilities to conduct full-scale imaging (neutron and x-ray) experiments of air cargo commodities and explosive benchmarks. (6) Conducted experiments to improve the collection of trace particles of explosives from a variety of surfaces representative of air cargo materials by means of mechanical (air/vibration/pressure), thermal, and electrostatic methods. Air cargo screening is a difficult challenge that will require significant investment in both research and development to find a suitable solution to ensure the safety of passengers without significantly hindering the flow of commodities. The initiative funded by Battelle has positioned this group to make major contributions in meeting the air cargo challenge by developing collaborations, developing laboratory test systems, improving knowledge of the challenges (both technical and business) for air cargo screening, and increasing the understanding of the capabilities for current inspection methods (x-ray radiography, x-ray backscatter, etc.) and potential future inspection methods (neutron radiography, fusion of detector modalities, advanced trace detection, etc.). Lastly, air cargo screening is still an issue that will benefit from collaboration between Department of Energy Laboratories and Battelle. On January 7, 2010, DHS Secretary Napolitano joined White House Press Secretary Robert Gibbs and Assistant to the President for Counterterrorism and Homeland Security John Brennan to announce several recommendations DHS has made to the President for improving the technology and procedures used to protect air travel from acts of terrorism. (This

  6. Vehicle and cargo inspection system

    Science.gov (United States)

    Verbinski, Victor V.; Orphan, Victor J.

    1997-02-01

    Vehicle and Cargo Inspection System (VACIS) is comprised of a 1 Curie 137Cs gamma-ray source in a shield and collimator which produces a fan-shaped beam designed to intercept a vertical array of gama-ray detectors contained in a tower structure. The source and detector modules straddle the vehicle or container being inspected and are mounted on self-propelled trolleys which travel in synchronization along two parallel tracks covering the length of the scanned object. The signals from the gamma-ray detector array are processed and displayed so as to produce a 2D gamma-radiographic image of the object. Testing of the system on a variety of empty and lightly-loaded vehicles and containers has demonstrated the effectiveness of VACIS in detecting hidden contraband. For example, a small sample of cocaine only 1.5 inches thick was readily detected in a container.

  7. 46 CFR 105.25-10 - Cargo pumping installation.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo pumping installation. 105.25-10 Section 105.25-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS... Installed Below Decks § 105.25-10 Cargo pumping installation. (a) Cargo pumps shall not be installed in...

  8. 46 CFR 154.412 - Cargo tank corrosion allowance.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank corrosion allowance. 154.412 Section 154.412... Containment Systems § 154.412 Cargo tank corrosion allowance. A cargo tank must be designed with a corrosion...) carries a cargo that corrodes the tank material. Note: Corrosion allowance for independent tank type C...

  9. 46 CFR 154.195 - Aluminum cargo tank: Steel enclosure.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Aluminum cargo tank: Steel enclosure. 154.195 Section... Equipment Hull Structure § 154.195 Aluminum cargo tank: Steel enclosure. (a) An aluminum cargo tank and its... the aluminum cargo tank must meet the steel structural standards of the American Bureau of...

  10. 46 CFR 154.562 - Cargo hose: Hydrostatic test.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo hose: Hydrostatic test. 154.562 Section 154.562 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY... Hose § 154.562 Cargo hose: Hydrostatic test. Each cargo hose must pass a hydrostatic pressure test...

  11. 29 CFR 1918.87 - Ship's cargo elevators.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Ship's cargo elevators. 1918.87 Section 1918.87 Labor... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Handling Cargo § 1918.87 Ship's cargo elevators. (a) Safe working load. The safe working loads of ship's cargo elevators shall be determined and...

  12. 46 CFR 153.977 - Supervision of cargo transfer.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Supervision of cargo transfer. 153.977 Section 153.977 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Procedures § 153.977 Supervision of cargo transfer. The person in charge of cargo transfer shall:...

  13. 46 CFR 151.13-5 - Cargo segregation-tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo segregation-tanks. 151.13-5 Section 151.13-5... CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Cargo Segregation § 151.13-5 Cargo segregation—tanks. (a... and list the various degrees of segregation required. Paragraphs and (2) of this section explain...

  14. 46 CFR 154.500 - Cargo and process piping standards.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo and process piping standards. 154.500 Section 154... SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Cargo and Process Piping Systems § 154.500 Cargo and process piping standards. The cargo...

  15. 41 CFR 102-117.140 - What is cargo preference?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What is cargo preference... cargo preference? Cargo preference is the statutory requirement that all, or a portion of all, ocean... the cargo preference laws must be approved by: Department of Transportation Maritime...

  16. DNA hairpins promote temperature controlled cargo encapsulation in a truncated octahedral nanocage structure family

    Science.gov (United States)

    Franch, Oskar; Iacovelli, Federico; Falconi, Mattia; Juul, Sissel; Ottaviani, Alessio; Benvenuti, Claudia; Biocca, Silvia; Ho, Yi-Ping; Knudsen, Birgitta R.; Desideri, Alessandro

    2016-07-01

    In the present study we investigate the mechanism behind temperature controlled cargo uptake using a truncated octahedral DNA cage scaffold functionalized with one, two, three or four hairpin forming DNA strands inserted in one corner of the structure. This investigation was inspired by our previous demonstration of temperature controlled reversible encapsulation of the cargo enzyme, horseradish peroxidase, in the cage with four hairpin forming strands. However, in this previous study the mechanism of cargo uptake was not directly addressed (Juul, et al., Temperature-Controlled Encapsulation and Release of an Active Enzyme in the Cavity of a Self-Assembled DNA Nanocage, ACS Nano, 2013, 7, 9724-9734). In the present study we use a combination of molecular dynamics simulations and in vitro analyses to unravel the mechanism of cargo uptake in hairpin containing DNA cages. We find that two hairpin forming strands are necessary and sufficient to facilitate efficient cargo uptake, which argues against a full opening-closing of one corner of the structure being responsible for encapsulation. Molecular dynamics simulations were carried out to evaluate the atomistic motions responsible for encapsulation and showed that the two hairpin forming strands facilitated extension of at least one of the face surfaces of the cage scaffold, allowing entrance of the cargo protein into the cavity of the structure. Hence, the presented data demonstrate that cargo uptake does not involve a full opening of the structure. Rather, the uptake mechanism represents a feature of increased flexibility integrated in this nanocage structure upon the addition of at least two hairpin-forming strands.In the present study we investigate the mechanism behind temperature controlled cargo uptake using a truncated octahedral DNA cage scaffold functionalized with one, two, three or four hairpin forming DNA strands inserted in one corner of the structure. This investigation was inspired by our previous

  17. System for inspection of stacked cargo containers

    Science.gov (United States)

    Derenzo, Stephen

    2011-08-16

    The present invention relates to a system for inspection of stacked cargo containers. One embodiment of the invention generally comprises a plurality of stacked cargo containers arranged in rows or tiers, each container having a top, a bottom a first side, a second side, a front end, and a back end; a plurality of spacers arranged in rows or tiers; one or more mobile inspection devices for inspecting the cargo containers, wherein the one or more inspection devices are removeably disposed within the spacers, the inspection means configured to move through the spacers to detect radiation within the containers. The invented system can also be configured to inspect the cargo containers for a variety of other potentially hazardous materials including but not limited to explosive and chemical threats.

  18. Design of heavy lift cargo aircraft

    Data.gov (United States)

    National Aeronautics and Space Administration — This is the bird of the skies of the future. The heavy lift cargo aircraft which is currently being developed by me has twice the payload capacity of an Antonov...

  19. IP-1 Certification of Cargo Containers

    Energy Technology Data Exchange (ETDEWEB)

    Hagler, Lisle [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2010-10-05

    The purpose and scope of this engineering note is to demonstrate that the structural design of the cargo container complies with the IP-1 container requirements of 49 CFR 173.410 as required by CFR 173.411.

  20. Characterizing X-ray Attenuation of Containerized Cargo

    Energy Technology Data Exchange (ETDEWEB)

    Birrer, N. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Divin, C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Glenn, S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Martz, H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wang, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-08-02

    X-ray inspection systems can be used to detect radiological and nuclear threats in imported cargo. In order to better understand performance of these systems, the attenuation characteristics of imported cargo need to be determined. This project focused on developing image processing algorithms for segmenting cargo and using x-ray attenuation to quantify equivalent steel thickness to determine cargo density. These algorithms were applied to over 450 cargo radiographs. The results are summarized in this report.

  1. EL VÍNCULO INDIVIDUO-COLECTIVO EN EL SISTEMA DE CARGOS EN UNA COMUNIDAD RURAL DEL SUR DE TLAXCALA, MÉXICO

    OpenAIRE

    Hernán Salas Quintanal; Iñigo González de la Fuente

    2013-01-01

    This paper exposes the authors reflections about their last investigations into  community organization concept. The research took place in several countries in Mexico from 2008 to 2012. It is an anthropological analysis about cargo system, which includes native and no-native communities. In such a way, the authors recognize the importance of heterogeneous institution into cargo system concept. So that, they suggest, as the main hypothesis, that cargo system creates several mechanisms, which ...

  2. Calcium and cargoes as regulators of myosin 5a activity

    International Nuclear Information System (INIS)

    Myosin 5a is a two-headed actin-dependent motor that transports various cargoes in cells. Its enzymology and mechanochemistry have been extensively studied in vitro. It is a processive motor that takes multiple 36 nm steps on actin. The enzymatic activity of myosin 5 is regulated by an intramolecular folding mechanism whereby its lever arms fold back against the coiled-coil tail such that the motor domains directly bind the globular tail domains. We show that the structure seen in individual folded molecules is consistent with electron density map of two-dimensional crystals of the molecule. In this compact state, the actin-activated MgATPase activity of the molecule is markedly inhibited and the molecule cannot move processively on surface bound actin filaments. The actin-activated MgATPase activity of myosin 5a is activated by increasing the calcium concentration or by binding of a cargo-receptor molecule, melanophilin, in vitro. However, calcium binding to the calmodulin light chains results in dissociation of some of the calmodulin which disrupts the ability of myosin 5a to move on actin filaments in vitro. Thus we propose that the physiologically relevant activation pathway in vivo involves binding of cargo-receptor proteins

  3. Quantifying the Dynamic Interactions Between a Clathrin-Coated Pit and Cargo Molecules

    Science.gov (United States)

    Weigel, Aubrey; Tamkun, Michael; Krapf, Diego

    2014-03-01

    Clathrin-mediated endocytosis is a major pathway of internalization of cargo in eukaryotic cells. This process involves the recruitment of cargo molecules into a growing clathrin-coated pit (CCP). However, cargo-CCP interactions are difficult to study because CCPs display a large degree of lifetime heterogeneity and the interactions with cargo molecules evolve over time. We use single-molecule total internal reflection fluorescence (TIRF) microscopy, in combination with automatic detection and tracking algorithms, to directly visualize the recruitment of individual voltage-gated potassium channels into forming CCPs in living cells. Contrary to widespread ideas, cargo often escapes from a pit before abortive CCP termination or endocytic vesicle production. By measuring tens of thousands of capturing events, we build the distribution of capture times and the times that cargo remains confined to a CCP. An analytical stochastic model is developed and compared to the measured distributions. Due to the dynamic nature of the pit, the model is non-Markovian and it displays long-tail power law statistics. Our findings identify one source of the large heterogeneities observed in CCP maturation and provide a mechanism for the anomalous diffusion of proteins in the plasma membrane. This work was supported by National Science Foundation Grant PHY-0956714.

  4. Failure of cargo aileron’s actuator

    Directory of Open Access Journals (Sweden)

    G. Zucca

    2014-10-01

    Full Text Available During a ferry flight, in a standard operation condition and at cruising level, a military cargo experienced a double hydraulic system failure due to a structural damage of the dual booster actuator. The booster actuator is the main component in mechanism of aileron’s deflection. The crew was able to arrange an emergency landing thanks to the spare oil onboard: load specialists refilled the hydraulic reservoirs. Due to safety concerns and in order to prevent the possibility of other similar incidents, a technical investigation took place. The study aimed to carry out the analysis of root causes of the actuator failure. The Booster actuator is composed mainly by the piston rod and its aluminum external case (AA7049. The assembly has two bronze caps on both ends. These are fixed in position by means of two retainers. At one end of the actuator case is placed a trunnion: a cylindrical protrusion used as a pivoting point on the aircraft. The fracture was located at one end of the case, on the trunnion side, in correspondence to the cap and over the retainer. One of the two fracture surfaces was found separated to the case and with the cap entangled inside. The fracture surfaces of the external case indicated fatigue crack growth followed by ductile separation. The failure analysis was performed by means of optical, metallographic, digital and electronic microscopy. The collected evidences showed a multiple initiation fracture mechanism. Moreover, 3D scanner reconstruction and numerical simulation demonstrated that dimensional non conformances and thermal loads caused an abnormal stress concentration. Stress concentration was located along the case assy outer surface where the fatigue crack originated. The progressive rupture mechanism grew under cyclical axial load due to the normal operations. Recommendations were issued in order to improve dimensional controls and assembly procedures during production and overhaul activities.

  5. Indirect estimation of radioactivity in containerized cargo

    International Nuclear Information System (INIS)

    Naturally occurring radioactive material in containerized cargo challenges the state of the art in national and international efforts to detect illicit nuclear and radiological material in transported containers. Current systems are being evaluated and new systems envisioned to provide the high probability of detection necessary to thwart potential threats, combined with extremely low nuisance and false alarm rates necessary to maintain the flow of commerce impacted by the enormous volume of commodities imported in shipping containers. Maintaining flow of commerce also means that inspection must be rapid, requiring relatively non-intrusive, indirect measurements of cargo from outside containers to the extent possible. With increasing information content in such measurements, it is natural to ask how the information might be combined to improve detection. Toward this end, we present an approach to estimating isotopic activity of naturally occurring radioactive material in cargo grouped by commodity type, combining container manifest data with radiography and gamma-ray spectroscopy aligned to location along the container. The heart of this approach is our statistical model of gamma-ray counts within peak regions of interest, which captures the effects of background suppression, counting noise, convolution of neighboring cargo contributions, and down-scattered photons to provide estimates of counts due to decay of specific radioisotopes in cargo alone. Coupled to that model, we use a mechanistic model of self-attenuated radiation flux to estimate the isotopic activity within cargo, segmented by location within each container, that produces those counts. We test our approach by applying it to a set of measurements taken at the Port of Seattle in 2006. This approach to synthesizing disparate available data streams and extraction of cargo characteristics, while relying on several simplifying assumptions and approximations, holds the potential to support improvement of

  6. Uncertainty Quantification for Cargo Hold Fires

    CERN Document Server

    DeGennaro, Anthony M; Martinelli, Luigi; Rowley, Clarence W

    2015-01-01

    The purpose of this study is twofold -- first, to introduce the application of high-order discontinuous Galerkin methods to buoyancy-driven cargo hold fire simulations, second, to explore statistical variation in the fluid dynamics of a cargo hold fire given parameterized uncertainty in the fire source location and temperature. Cargo hold fires represent a class of problems that require highly-accurate computational methods to simulate faithfully. Hence, we use an in-house discontinuous Galerkin code to treat these flows. Cargo hold fires also exhibit a large amount of uncertainty with respect to the boundary conditions. Thus, the second aim of this paper is to quantify the resulting uncertainty in the flow, using tools from the uncertainty quantification community to ensure that our efforts require a minimal number of simulations. We expect that the results of this study will provide statistical insight into the effects of fire location and temperature on cargo fires, and also assist in the optimization of f...

  7. Characterizing Complexity of Containerized Cargo X-ray Images

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Guangxing [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Martz, Harry [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Glenn, Steven [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Divin, Charles [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Birrer, Nat [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-08-19

    X-ray imaging can be used to inspect cargos imported into the United States. In order to better understand the performance of X-ray inspection systems, the X-ray characteristics (density, complexity) of cargo need to be quantified. In this project, an image complexity measure called integrated power spectral density (IPSD) was studied using both DNDO engineered cargos and stream-of-commerce (SOC) cargos. A joint distribution of cargo density and complexity was obtained. A support vector machine was used to classify the SOC cargos into four categories to estimate the relative fractions.

  8. Fluctuation effects in bidirectional cargo transport

    CERN Document Server

    Klein, Sarah; Santen, Ludger

    2014-01-01

    We discuss a theoretical model for bidirectional cargo transport in biological cells, which is driven by teams of molecular motors and subject to thermal fluctuations. The model describes explicitly the directed motion of the molecular motors on the filament. The motor-cargo coupling is implemented via linear springs. By means of extensive Monte Carlo simulations we show that the model describes the experimentally observed regimes of anomalous diffusion, i.e. subdiffusive behavior at short times followed by superdiffusion at intermediate times. The model results indicate that subdiffuse regime is induced by thermal fluctuations while the superdiffusive motion is generated by correlations of the motors' activity. We also tested the efficiency of bidirectional cargo transport in crowded areas by measuring its ability to pass barriers with increased viscosity. Our results show a remarkable gain of efficiency for high viscosities.

  9. Institute Cargo Clauses : Nova verzija 2009.

    OpenAIRE

    Lovrić, Ivana

    2012-01-01

    Institute Cargo Clauses, 2009. znače izmijenjene i dopunjene Institute Cargo Clauses iz 1982. Osnovni razlozi za reviziju prijašnjih uvjeta leže u pojavi novih rizika, potrebi da se uvjeti kargo osiguranja prilagode promjenama u suvremenom transportnom sustavu te jezično i terminološki osuvremene. Novi uvjeti donose niz rješenja koja znače modernizaciju u odnosu na rješenja Marine Insurance Acta iz 1906. Redaktori novih uvjeta vodili su računa i o tome da pojedine odredbe budu jasnije form...

  10. Trajanje osiguranja prema Institute Cargo Clauses (2009.)

    OpenAIRE

    Petrinović, Ranka; Lovrić, Ivana

    2012-01-01

    Institute Cargo Clauses iz 2009. zapravo su izmjene i dopune Institute Cargo Clauses iz 1982. Osnovni su razlozi za reviziju prijašnjih uvjeta u pojavi novih rizika, potrebi da se uvjeti kargo-osiguranja prilagode promjenama u suvremenome transportnom sustavu te jezično i terminološki osuvremene. Novi uvjeti donose više rješenja koja znače modernizaciju u usporedbi s onima u Marine Insurance Acta iz 1906., pa se prezentiraju kao klauzule za novo tisućljeće. Među bitnim promjenama koje donose ...

  11. 46 CFR 105.20-3 - Cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... VESSELS DISPENSING PETROLEUM PRODUCTS Specific Requirements-Cargo Tanks § 105.20-3 Cargo tanks. (a... subjected, except that in no case shall the thickness of the shell or head be less than that specified...

  12. 49 CFR 172.448 - CARGO AIRCRAFT ONLY label.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false CARGO AIRCRAFT ONLY label. 172.448 Section 172.448... SECURITY PLANS Labeling § 172.448 CARGO AIRCRAFT ONLY label. (a) Except for size and color, the CARGO AIRCRAFT ONLY label must be as follows: ER14JA09.001 (b) The CARGO AIRCRAFT ONLY label must be black on...

  13. 46 CFR 151.25-2 - Cargo handling space.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo handling space. 151.25-2 Section 151.25-2 Shipping... BULK LIQUID HAZARDOUS MATERIAL CARGOES Environmental Control § 151.25-2 Cargo handling space. Pump rooms, compressor rooms, refrigeration rooms, heating rooms, instrument rooms or other closed...

  14. 33 CFR 157.23 - Cargo and ballast system information.

    Science.gov (United States)

    2010-07-01

    ... CARRYING OIL IN BULK Design, Equipment, and Installation § 157.23 Cargo and ballast system information. (a... automatic and manual operation of the cargo and ballast system in the vessel. (b) The format and information... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Cargo and ballast...

  15. 46 CFR 153.440 - Cargo temperature sensors.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo temperature sensors. 153.440 Section 153.440 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Temperature Control Systems § 153.440 Cargo temperature sensors. (a) Except as prescribed in paragraph (c)...

  16. 48 CFR 52.228-9 - Cargo Insurance.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Cargo Insurance. 52.228-9... Insurance. As prescribed in 28.313(a), insert the following clause: Cargo Insurance (MAY 1999) (a) The..., cargo insurance of $_____ per vehicle to cover the value of property on each vehicle and of $_____...

  17. 46 CFR 151.20-20 - Cargo transfer methods.

    Science.gov (United States)

    2010-10-01

    ... 90 percent of the set pressure of the cargo tank safety relief valve. The pressurizing line shall be... shall be fitted with a safety relief valve set to lift at a pressure no higher than 80 percent of the cargo tank safety relief valve setting. (d) When cargo vapors are flammable, combustible or toxic,...

  18. THE ANALYSIS OF THE LOGISTICS ORGANIZATION OF CUSTOMS CARGO PROCESSING

    OpenAIRE

    N. Ponomariova; Stoliar, T.

    2008-01-01

    The matter concerning customs logistics being an integral part of cargo delivery logistics range of international routes has been considered. The aim of functioning of cargo customs handling process has been determined. The application of Petri network theory for the purpose of customs cargo handling model development has been proved.

  19. Development of 60Co cargo train inspection system

    International Nuclear Information System (INIS)

    The author introduces the research and development of 60Co cargo train inspection system. With the use of radiography principle, every car image is acquired when the cargo train runs through the inspection channel. It is evaluated whether the cargo in car matches the corresponding customs declaration information with digital image processing techniques. The system has been installed in railway port at Manzhouli Customs

  20. Molecular approach to intracellular cargo transport

    Science.gov (United States)

    Yildiz, Ahmet

    2010-03-01

    Landmark discoveries in the study of cytoplasmic motors have been made through advances in single molecule biophysics and detailed mechanistic models exist for kinesin and dynein. However, the function of motors in physiological conditions has not been carefully tested. In cells, more than few dyneins can attach to the same cargo and interact with the opposite polarity motors of kinesin. To study the molecular crosstalk between the motors, we have used intraflagellar transport (IFT) in Chlamydomonas reinhardtii as a model system. Ultrahigh spatio-temporal tracking of single cargo movement showed that IFT particles move for long distances unidirectionally with 8 nm increments, agreeing with measured step sizes of kinesin and dynein. To measure how many motors transport each cargo, we have linked large polystyrene beads to internal IFT particles through a transmembrane protein. Force measurements indicated that, on average, 3-4 motors transport cargoes in each direction. The results showed that IFT motors are tightly coordinated and might be involved in recycling each other to the appropriate end of the flagellum.

  1. 78 FR 68784 - Cargo Securing Manuals

    Science.gov (United States)

    2013-11-15

    ... regarding our public dockets in the January 17, 2008, issue of the Federal Register (73 FR 3316). D. Public... Practice for Cargo Stowage and Securing E.O. Executive Order FR Federal Register IMO International Maritime.... vessels or for foreign vessels operating in U.S. waters. In a notice (64 FR 1648; Jan. 11,...

  2. 46 CFR 64.91 - Relief valve for the cargo pump discharge.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Relief valve for the cargo pump discharge. 64.91 Section... PORTABLE TANKS AND CARGO HANDLING SYSTEMS Cargo Handling System § 64.91 Relief valve for the cargo pump discharge. The cargo pump discharge must have a relief valve that is— (a) Fitted between the cargo...

  3. Discharging Part of LNG Liquid Cargo Handling Simulator

    Directory of Open Access Journals (Sweden)

    Zhen-Zhou Yang

    2013-09-01

    Full Text Available With the development of the national economy, it is important to develop LNG (Liquefied Natural Gas liquid cargo handling simulator. In this paper, the discharging part of Liquid Cargo Handling Simulator of LNG Carriers is developed by Visual Basic. The system includes 5 major forms and 3 mathematical models such as cargo pump model, the pressure and temperature model of the LNG vapour in the cargo tank and the liquid level model. The system can complete the unloading task. The system has become a preliminary model of LNG liquid cargo handlings simulator training.

  4. Aviation System Analysis Capability Air Carrier Investment Model-Cargo

    Science.gov (United States)

    Johnson, Jesse; Santmire, Tara

    1999-01-01

    The purpose of the Aviation System Analysis Capability (ASAC) Air Cargo Investment Model-Cargo (ACIMC), is to examine the economic effects of technology investment on the air cargo market, particularly the market for new cargo aircraft. To do so, we have built an econometrically based model designed to operate like the ACIM. Two main drivers account for virtually all of the demand: the growth rate of the Gross Domestic Product (GDP) and changes in the fare yield (which is a proxy of the price charged or fare). These differences arise from a combination of the nature of air cargo demand and the peculiarities of the air cargo market. The net effect of these two factors are that sales of new cargo aircraft are much less sensitive to either increases in GDP or changes in the costs of labor, capital, fuel, materials, and energy associated with the production of new cargo aircraft than the sales of new passenger aircraft. This in conjunction with the relatively small size of the cargo aircraft market means technology improvements to the cargo aircraft will do relatively very little to spur increased sales of new cargo aircraft.

  5. Detection of Neutron Sources in Cargo Containers

    OpenAIRE

    Katz, J. I.

    2007-01-01

    We investigate the problem of detecting the presence of clandestine neutron sources, such as would be produced by nuclear weapons containing plutonium, within cargo containers. Small, simple and economical semiconductor photodiode detectors affixed to the outsides of containers are capable of producing statistically robust detections of unshielded sources when their output is integrated over the durations of ocean voyages. It is possible to shield such sources with thick layers of neutron-abs...

  6. Design of a spanloader cargo aircraft

    Science.gov (United States)

    1989-01-01

    With a growing demand for fast international freight service, the slow-moving cargo ships currently in use will soon find a substantial portion of their clients looking elsewhere. One candidate for filling this expected gap in the freight market is a span-loading aircraft (or 'flying wing') capable of long-range operation with extremely large payloads. This report summarizes the design features of an aircraft capable of fulfilling a long-haul, high-capacity cargo mission. The spanloader seeks to gain advantage over conventional aircraft by eliminating the aircraft fuselage and thus reducing empty weight. The primary disadvantage of this configuration is that the cargo-containing wing tends to be thick, thus posing a challenge to the airfoil designer. It also suffers from stability and control problems not encountered by conventional aircraft. The result is an interesting, challenging exercise in unconventional design. The report that follows is a student written synopsis of an effort judged to be the best of eight designs developed during the year 1988-1989.

  7. Local synaptic signaling enhances the stochastic transport of motor-driven cargo in neurons

    KAUST Repository

    Newby, Jay

    2010-08-23

    The tug-of-war model of motor-driven cargo transport is formulated as an intermittent trapping process. An immobile trap, representing the cellular machinery that sequesters a motor-driven cargo for eventual use, is located somewhere within a microtubule track. A particle representing a motor-driven cargo that moves randomly with a forward bias is introduced at the beginning of the track. The particle switches randomly between a fast moving phase and a slow moving phase. When in the slow moving phase, the particle can be captured by the trap. To account for the possibility that the particle avoids the trap, an absorbing boundary is placed at the end of the track. Two local signaling mechanisms-intended to improve the chances of capturing the target-are considered by allowing the trap to affect the tug-of-war parameters within a small region around itself. The first is based on a localized adenosine triphosphate (ATP) concentration gradient surrounding a synapse, and the second is based on a concentration of tau-a microtubule-associated protein involved in Alzheimer\\'s disease-coating the microtubule near the synapse. It is shown that both mechanisms can lead to dramatic improvements in the capture probability, with a minimal increase in the mean capture time. The analysis also shows that tau can cause a cargo to undergo random oscillations, which could explain some experimental observations. © 2010 IOP Publishing Ltd.

  8. Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle.

    Science.gov (United States)

    Bertipaglia, Chiara; Schneider, Sarah; Jakobi, Arjen J; Tarafder, Abul K; Bykov, Yury S; Picco, Andrea; Kukulski, Wanda; Kosinski, Jan; Hagen, Wim Jh; Ravichandran, Arvind C; Wilmanns, Matthias; Kaksonen, Marko; Briggs, John Ag; Sachse, Carsten

    2016-07-01

    Selective autophagy is the mechanism by which large cargos are specifically sequestered for degradation. The structural details of cargo and receptor assembly giving rise to autophagic vesicles remain to be elucidated. We utilize the yeast cytoplasm-to-vacuole targeting (Cvt) pathway, a prototype of selective autophagy, together with a multi-scale analysis approach to study the molecular structure of Cvt vesicles. We report the oligomeric nature of the major Cvt cargo Ape1 with a combined 2.8 Å X-ray and negative stain EM structure, as well as the secondary cargo Ams1 with a 6.3 Å cryo-EM structure. We show that the major dodecameric cargo prApe1 exhibits a tendency to form higher-order chain structures that are broken upon interaction with the receptor Atg19 in vitro The stoichiometry of these cargo-receptor complexes is key to maintaining the size of the Cvt aggregate in vivo Using correlative light and electron microscopy, we further visualize key stages of Cvt vesicle biogenesis. Our findings suggest that Atg19 interaction limits Ape1 aggregate size while serving as a vehicle for vacuolar delivery of tetrameric Ams1. PMID:27266708

  9. Self-propelling surfactant droplets in chemically-confined microfluidics--cargo transport, drop-splitting and trajectory control.

    Science.gov (United States)

    Sinz, David K N; Darhuber, Anton A

    2012-02-21

    We demonstrate the applicability of self-propulsion as a passive driving mechanism for droplets in chemically-confined microfluidics. The droplets can be used to transport considerably sized solid cargo particles. We implemented thermal actuation as a steering mechanism for the droplets at fluidic junctions. PMID:22246050

  10. Cargo transportation by two species of motor protein

    CERN Document Server

    Zhang, Yunxin

    2012-01-01

    The cargo motion in living cells transported by two species of motor protein with different intrinsic directionality is discussed in this study. Similar to single motor movement, cargo steps forward and backward along microtubule stochastically. Recent experiments found that, cargo transportation by two motor species has a memory, it does not change its direction as frequently as expected, which means that its forward and backward step rates depends on its previous motion trajectory. By assuming cargo has only the least memory, i.e. its step direction depends only on the direction of its last step, two cases of cargo motion are detailed analyzed in this study: {\\bf (I)} cargo motion under constant external load; and {\\bf (II)} cargo motion in one fixed optical trap. Due to the existence of memory, for the first case, cargo can keep moving in the same direction for a long distance. For the second case, the cargo will oscillate in the trap. The oscillation period decreases and the oscillation amplitude increase...

  11. Local synaptic signaling enhances the stochastic transport of motor-driven cargo in neurons

    Science.gov (United States)

    Newby, Jay; Bressloff, Paul C.

    2010-09-01

    The tug-of-war model of motor-driven cargo transport is formulated as an intermittent trapping process. An immobile trap, representing the cellular machinery that sequesters a motor-driven cargo for eventual use, is located somewhere within a microtubule track. A particle representing a motor-driven cargo that moves randomly with a forward bias is introduced at the beginning of the track. The particle switches randomly between a fast moving phase and a slow moving phase. When in the slow moving phase, the particle can be captured by the trap. To account for the possibility that the particle avoids the trap, an absorbing boundary is placed at the end of the track. Two local signaling mechanisms—intended to improve the chances of capturing the target—are considered by allowing the trap to affect the tug-of-war parameters within a small region around itself. The first is based on a localized adenosine triphosphate (ATP) concentration gradient surrounding a synapse, and the second is based on a concentration of tau—a microtubule-associated protein involved in Alzheimer's disease—coating the microtubule near the synapse. It is shown that both mechanisms can lead to dramatic improvements in the capture probability, with a minimal increase in the mean capture time. The analysis also shows that tau can cause a cargo to undergo random oscillations, which could explain some experimental observations.

  12. Local synaptic signaling enhances the stochastic transport of motor-driven cargo in neurons

    International Nuclear Information System (INIS)

    The tug-of-war model of motor-driven cargo transport is formulated as an intermittent trapping process. An immobile trap, representing the cellular machinery that sequesters a motor-driven cargo for eventual use, is located somewhere within a microtubule track. A particle representing a motor-driven cargo that moves randomly with a forward bias is introduced at the beginning of the track. The particle switches randomly between a fast moving phase and a slow moving phase. When in the slow moving phase, the particle can be captured by the trap. To account for the possibility that the particle avoids the trap, an absorbing boundary is placed at the end of the track. Two local signaling mechanisms—intended to improve the chances of capturing the target—are considered by allowing the trap to affect the tug-of-war parameters within a small region around itself. The first is based on a localized adenosine triphosphate (ATP) concentration gradient surrounding a synapse, and the second is based on a concentration of tau—a microtubule-associated protein involved in Alzheimer's disease—coating the microtubule near the synapse. It is shown that both mechanisms can lead to dramatic improvements in the capture probability, with a minimal increase in the mean capture time. The analysis also shows that tau can cause a cargo to undergo random oscillations, which could explain some experimental observations

  13. APPL endosomes are not obligatory endocytic intermediates but act as stable cargo-sorting compartments

    Science.gov (United States)

    Kalaidzidis, Inna; Miaczynska, Marta; Brewińska-Olchowik, Marta; Hupalowska, Anna; Ferguson, Charles; Parton, Robert G.; Kalaidzidis, Yannis

    2015-01-01

    Endocytosis allows cargo to enter a series of specialized endosomal compartments, beginning with early endosomes harboring Rab5 and its effector EEA1. There are, however, additional structures labeled by the Rab5 effector APPL1 whose role in endocytic transport remains unclear. It has been proposed that APPL1 vesicles are transport intermediates that convert into EEA1 endosomes. Here, we tested this model by analyzing the ultrastructural morphology, kinetics of cargo transport, and stability of the APPL1 compartment over time. We found that APPL1 resides on a tubulo-vesicular compartment that is capable of sorting cargo for recycling or degradation and that displays long lifetimes, all features typical of early endosomes. Fitting mathematical models to experimental data rules out maturation of APPL1 vesicles into EEA1 endosomes as a primary mechanism for cargo transport. Our data suggest instead that APPL1 endosomes represent a distinct population of Rab5-positive sorting endosomes, thus providing important insights into the compartmental organization of the early endocytic pathway. PMID:26459602

  14. STRATEGIC MANAGEMENT OF TRANSPORT CARGO COMPLEX

    Directory of Open Access Journals (Sweden)

    A. M. Okorokov

    2014-06-01

    Full Text Available Purpose. Making the qualitative administrative decisions defining strategy and tactics of transport cargo complexes development, and also its subsystems, is possible only in the presence of flexible optimization model. This model has to consider multiparametricity and multicriteriality of the given task, uncertainty and vagueness of input information, and also to provide process automation of searching the best parameters of the given production facility. The purpose of the research is to develop procedures for the strategic management of complex with view of the most important factors and their stochastic nature, which will execute the improvement of technical equipment of TCC. Methodology. The problem of strategic management is based on solving the complex of issues of the optimal number of shunting locomotives, optimal processing capability of handling the front and rational capacity of warehouses. The problem is solved on the basis of the proposed optimality criterion – the specific set of profit per unit of capital assets of freight industry. The listed problems are solved using simulation modeling of the freight industry. Findings. The use of developed procedure allows one to improve the technical equipment of the freight stations and complexes. Originality. For the first time it was developed the procedure of strategic management of development. This procedure allows taking into account the probabilistic nature of demand for services of transport freight complexes and technological processes of client services on the complex stations. The proposed procedure can be applied during when planning the investments in the creation of transport freight complexes. Practical value. Use as a basic tool of simulation models of complex cargo operation allows estimating the effectiveness of the capital investments, the level of operating costs, as well as the quality of meeting the demands of potential customers in transportations at the stage of

  15. NLS cargo transfer vehicle propulsion system

    Science.gov (United States)

    Hearn, Hank C.; Langford, G. K.

    1992-02-01

    The propulsion system of the Cargo Transfer Vehicle is designed to meet a wide range of requirements associated with the National Launch System (NLS) resupply function for Space Station Freedom. It provides both orbit adjustment and precise vehicle control capability, and is compatible with close proximity operation at the space station as well as return on the shuttle for ground refurbishment and reuse. Preliminary trade studies have resulted in designing and sizing an integrated bipropellant system using monomethyl hydrazine and nitrogen tetroxide. Design and analysis activities are continuing, and the design will evolve and mature as part of the NLS program.

  16. Electronic Method to Detect Persons Hidden Within Cargo containers

    Energy Technology Data Exchange (ETDEWEB)

    Fuller, G.M.

    1997-11-10

    Physical security technologies have been unable to address most `Trojan Horse' scenarios at vehicle portals which provide access through the perimeter of a secure area. Thorough visual searches of vehicle cargo containers are time consuming, involve a number of security personnel and are cursory at best. Vehicles entering or exiting a protected site provide an adversary with an easy pathway across secure boundaries. A method to detect the presence of persons hiding within a vehicle's enclosed cargo container has been developed by LQckheed Martin Energy Systems. The system measures vibrations coupled to the container and generated by the human heart. Each time the human heart beats, it generates a small but measurable shock wave. This shock wave is propagated through the body and transmitted to anything with which the body has contact. This wave is referred to as a baflistocardiograrn and is the mechanical equivalent to an electrocardiogram. Systems have been installed in several State prisons and have been independently tested and evaluated. The effectiveness of the system has been determined by the Thunder Mountain Evaluation Center at Fort Huachuca. Arizona. Sympathetic vibrations of the cargo container's surface can be collected using any of several detection methods, i.e. infrared, Doppler microwave, Doppler ultrasonic wave and geophones. The analog signal delivered by the sensors is passed through an amplifier and a low pass filter and then fed to a microprocessor via an analog to digital converter. When initiated by the operator, anafog signals are recorded for a specific interval of time and rate. At the end of the recording intervaL the data are analysed using wavelet transform techniques and compared with a ballistocardiographic template. If the collected data are similar, the system informs the operator that there is a high degree of probability that a person is within the vehicle. The paper describes in depth the development of the system

  17. Transportation of nanoscale cargoes by myosin propelled actin filaments.

    Directory of Open Access Journals (Sweden)

    Malin Persson

    Full Text Available Myosin II propelled actin filaments move ten times faster than kinesin driven microtubules and are thus attractive candidates as cargo-transporting shuttles in motor driven lab-on-a-chip devices. In addition, actomyosin-based transportation of nanoparticles is useful in various fundamental studies. However, it is poorly understood how actomyosin function is affected by different number of nanoscale cargoes, by cargo size, and by the mode of cargo-attachment to the actin filament. This is studied here using biotin/fluorophores, streptavidin, streptavidin-coated quantum dots, and liposomes as model cargoes attached to monomers along the actin filaments ("side-attached" or to the trailing filament end via the plus end capping protein CapZ. Long-distance transportation (>100 µm could be seen for all cargoes independently of attachment mode but the fraction of motile filaments decreased with increasing number of side-attached cargoes, a reduction that occurred within a range of 10-50 streptavidin molecules, 1-10 quantum dots or with just 1 liposome. However, as observed by monitoring these motile filaments with the attached cargo, the velocity was little affected. This also applied for end-attached cargoes where the attachment was mediated by CapZ. The results with side-attached cargoes argue against certain models for chemomechanical energy transduction in actomyosin and give important insights of relevance for effective exploitation of actomyosin-based cargo-transportation in molecular diagnostics and other nanotechnological applications. The attachment of quantum dots via CapZ, without appreciable modulation of actomyosin function, is useful in fundamental studies as exemplified here by tracking with nanometer accuracy.

  18. Information tecnology and its impact on port cargo distribution businness

    OpenAIRE

    Trampus, Francesca

    2002-01-01

    The IT revolution has dynamised the port cargo business: up-to-date software is needed to optimize operations such as vessel loading, yard planning, equipment flow, gate handling, administration and invoicing and it is also essential for vessel tracking, electronic declaration of cargo and passengers and as a communication platform to all port users.

  19. 29 CFR 1917.14 - Stacking of cargo and pallets.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Stacking of cargo and pallets. 1917.14 Section 1917.14 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... pallets. Cargo, pallets and other material stored in tiers shall be stacked in such a manner as to...

  20. 33 CFR 155.775 - Maximum cargo level of oil.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Maximum cargo level of oil. 155.775 Section 155.775 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Personnel, Procedures, Equipment, and Records § 155.775 Maximum cargo level of oil. (a) For the purposes...

  1. Environmental control of microtubule-based bidirectional cargo-transport

    CERN Document Server

    Klein, Sarah; Santen, Ludger

    2014-01-01

    Inside cells, various cargos are transported by teams of molecular motors. Intriguingly, the motors involved generally have opposite pulling directions, and the resulting cargo dynamics is a biased stochastic motion. It is an open question how the cell can control this bias. Here we develop a model which takes explicitly into account the elastic coupling of the cargo with each motor. We show that bias can be simply controlled or even reversed in a counterintuitive manner via a change in the external force exerted on the cargo or a variation of the ATP binding rate to motors. Furthermore, the superdiffusive behavior found at short time scales indicates the emergence of motor cooperation induced by cargo-mediated coupling.

  2. Fatalities to occupants of cargo areas of pickup trucks.

    Science.gov (United States)

    Anderson, C L; Agran, P F; Winn, D G; Greenland, S

    2000-07-01

    We sought to describe the fatalities to occupants of pickup truck cargo areas and to compare the mortality of cargo area occupants to passengers in the cab. From the Fatality Analysis Reporting System (FARS) files for 1987-1996, we identified occupants of pickup trucks with at least one fatality and at least one passenger in the cargo area. Outcomes of cargo area occupants and passengers in the cab were compared using estimating equations conditional on the crash and vehicle. Thirty-four percent of deaths to cargo occupants were in noncrash events without vehicle deformation. Fifty-five percent of those who died were age 15-29 years and 79% were male. The fatality risk ratio (FRR) comparing cargo area occupants to front seat occupants was 3.0 (95% Confidence Interval [CI] = 2.7-3.4). The risk was 7.9 (95% CI = 6.2-10.1) times that of restrained front seat occupants. The FRR ranged from 92 (95% CI = 47-179) in noncrash events to 1.7 (95% CI = 1.5-1.9) in crashes with severe vehicle deformation. The FRR was 1.8 (95% CI = 1.4-2.3) for occupants of enclosed cargo areas and 3.5 (95% CI = 3.1-4.0) for occupants of open cargo areas. We conclude that passengers in cargo areas of pickup trucks have a higher risk of death than front seat occupants, especially in noncrash events, and that camper shells offer only limited protection for cargo area occupants. PMID:10868756

  3. 49 CFR 1546.213 - Access to cargo: Security threat assessments for cargo personnel in the United States.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Access to cargo: Security threat assessments for...: Security threat assessments for cargo personnel in the United States. This section applies in the United...— (1) Each individual must successfully complete a security threat assessment or comparable...

  4. 49 CFR 1548.15 - Access to cargo: Security threat assessments for individuals having unescorted access to cargo.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Access to cargo: Security threat assessments for... SECURITY CIVIL AVIATION SECURITY INDIRECT AIR CARRIER SECURITY § 1548.15 Access to cargo: Security threat... must successfully complete a security threat assessment or comparable security threat...

  5. 46 CFR 154.1200 - Mechanical ventilation system: General.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Mechanical ventilation system: General. 154.1200 Section... Equipment Cargo Area: Mechanical Ventilation System § 154.1200 Mechanical ventilation system: General. (a... cargo handling equipment must have a fixed, exhaust-type mechanical ventilation system. (b)...

  6. Distinct N-terminal regions of the exomer secretory vesicle cargo Chs3 regulate its trafficking itinerary

    Directory of Open Access Journals (Sweden)

    J. ChristopherFromme

    2014-09-01

    Full Text Available Cells transport integral membrane proteins between organelles by sorting them into vesicles. Cargo adaptors act to recognize sorting signals in transmembrane cargos and to interact with coat complexes that aid in vesicle biogenesis. No coat proteins have yet been identified that generate secretory vesicles from the trans-Golgi network (TGN to the plasma membrane, but the exomer complex has been identified as a cargo adaptor complex that mediates transport of several proteins in this pathway. Chs3, the most well-studied exomer cargo, cycles between the TGN and the plasma membrane in synchrony with the cell cycle, providing an opportunity to study regulation of proteins that cycle in response to signaling. Here we show that different segments of the Chs3 N-terminus mediate distinct trafficking steps. Residues 10-27, known to mediate retention, also appear to play a role in internalization. Residues 28-52 are involved in transport to the plasma membrane and recycling out of endosomes to prevent degradation in the vacuole. We also present the crystal structure of residues 10-27 bound to the exomer complex, suggesting different cargo adaptors could compete for binding to this segment, providing a potential mechanism for regulation.

  7. Transporting "exceptional cargo" on the CERN sites

    CERN Multimedia

    EN Department

    2012-01-01

    When the Transport Service is managing "exceptional cargo", the driver and the escort are often in charge of an operation involving equipment worth many hundred thousand francs. Equipment that may well be irreplaceable for a facility or an experiment.   The members of the Transport Service who carry out these tasks are very professional and are – needless to say – highly concentrated on the job. They count on your understanding and support in the traffic on site. Their convoys are – for good reasons – moving slowly. Kindly do not overtake, do not cut in in front of them and do not drive too closely. Respect the escort and do not position yourself between the truck and the escort vehicles. The EN department counts on your courtesy on the road.  

  8. A Liquid Cargo Handling Training Simulator

    Directory of Open Access Journals (Sweden)

    Malvin Villabø

    1982-01-01

    Full Text Available This paper describes a computer-based, real-time training simulator for liquid cargo handling. The mathematical model description is modular and emphasizes the flow generating part. The flow model applies network theory to describe the logical piping connections and the overall flow system performance, while Bernoulli's equation is used for the description of the piping elements. Numerically the Newton-Raphson method is applied for computation of the non-linear flow model. The programming language is HP-FORTRAN IV and the simulator is implemented on an HP minicomputer system. The instructor part of the system is interactive and controlled by the use of a standard ASCII terminal. The simulation part of the system is running in real-time or scaled real-time, and is controlled from the trainee's panel.

  9. Applications and Challenges for Use of Cell-Penetrating Peptides as Delivery Vectors for Peptide and Protein Cargos

    DEFF Research Database (Denmark)

    Kristensen, Mie; Birch, Ditlev; Mørck Nielsen, Hanne

    2016-01-01

    -penetrating peptides (CPPs) constitute a promising tool and have shown applications for peptide and protein delivery into cells as well as across various epithelia and the blood-brain barrier (BBB). CPP-mediated delivery of peptides and proteins may be pursued via covalent conjugation of the CPP to the cargo peptide...... or protein or via physical complexation obtained by simple bulk-mixing of the CPP with its cargo. Both approaches have their pros and cons, and which is the better choice likely relates to the physicochemical properties of the CPP and its cargo as well as the route of administration, the specific...... barrier and the target cell. Besides the physical barrier, a metabolic barrier must be taken into consideration when applying peptide-based delivery vectors, such as the CPPs, and stability-enhancing strategies are commonly employed to prolong the CPP half-life. The mechanisms by which CPPs translocate...

  10. Injuries to occupants in cargo areas of pickup trucks.

    Science.gov (United States)

    Agran, P; Winn, D; Anderson, C

    1994-11-01

    Transporting passengers in the cargo area of pickup trucks is a public health safety issue in the United States. Our study compared crashes involving passengers in the cargo area with those involving passengers in the cab. We obtained data for all injury events of pickup occupants for 1990 from the California Highway Patrol. A total of 702 traffic reports coded as having passengers riding in pickup truck beds involved 1,685 passengers in the cargo area and 865 in the cab. Significantly more events involving passengers in the cargo area occurred in summer in rural areas and were noncollisions than did events with only cab passengers. Crashes with passengers in the cargo area resulted in death in 5% of passengers. Of the drivers, 81% were male and 22% were younger than 20 years. Among the 1,685 passengers in the cargo area, 65% were male, 36% were younger than 15 years, and 30% were ages 15 to 19 years. Passengers in the cargo area were more frequently ejected and more seriously injured than their counterparts in the cab. Legislation to restrict travel in truck beds and the design of restraints for this area are some measures that may reduce the risk of injury. PMID:7810125

  11. A UAV mechanism for autonomous landingand transportation of cargo

    OpenAIRE

    Stefánsson, Tryggvi

    2014-01-01

    Anvandningen av robotar blir allt vanligare i vardagen och robotar kan klara av alltmer komplexa jobb. Obemannade luftfartyg (UAV) ar en del av denna revolution och de dominerar luftrummet i robotomradet. Framsteg inom elektronik har i hog grad bidragit till de snabba framsteg i robotar och UAV, genom att minska kostnaderna och samtidigt oka kapacitet och minska stromforbrukningen av komponenter. Gemenskapenfor oppen kallkod har dessutom i hog grad bidragit till utvecklingen och anvandningena...

  12. On the possibility of the in-cell molecular motors working as cargo moorings only

    CERN Document Server

    Lisowski, Bartosz

    2012-01-01

    Although active transport is considered the most efficient way of depositing materials in the appropriate compartments within living cells, free diffusion remains the energetically cheapest and most widespread determinant of the in-cell dynamics. Here we investigate the hypothesis of cargoes - vesicles or organelles - being transported by the means of free diffusion, limited spatially by the molecular motors. Routinely navigating through ever-changing and unsteady environment, utilizing chemical energy (e.g. from hydrolyzing ATP), as the result they transport cell's crucial components, such as neurotransmitters and organelles. In our model the motor, which we identify with kinesin-1 walking along the microtubule, tethers the cargo in the same manner as a mooring rope attached to a bollard holds a ship from drifting toward the open sea. We study the model behavior in the context of optical trap single molecule experiments, mimicking their procedures in our simulations and investigate how the mechanical propert...

  13. Technical and economic evaluation of advanced air cargo system concepts

    Science.gov (United States)

    Whitehead, A. H., Jr.

    1977-01-01

    The paper reviews NASA air cargo market studies, reports on NASA and NASA-sponsored studies of advanced freighter concepts, and identifies the opportunities for the application of advanced technology. The air cargo market is studied to evaluate the timing for, and the potential market response to, advanced technology aircraft. The degree of elasticity in future air freight markets is also being investigated, since the demand for a new aircraft is most favorable in a price-sensitive environment. Aircraft design studies are considered with attention to mission and design requirements, incorporation of advanced technologies in transport aircraft, new cargo aircraft concepts, advanced freighter evaluation, and civil-military design commonality.

  14. Cell-Penetrating Peptides: A Comparative Study on Lipid Affinity and Cargo Delivery Properties

    Directory of Open Access Journals (Sweden)

    Paolo Ruzza

    2010-03-01

    Full Text Available A growing number of natural and/or synthetic peptides with cell membrane penetrating capability have been identified and described in the past years. These molecules have been considered promising tools for delivering bioactive compounds into various cell types. Although the mechanism of uptake is still unclear, it is reasonable to assume that the relative contribute of each proposed mechanism could differ for the same peptide, depending on experimental protocol and cargo molecule composition. In this work we try to connect the capability to interact with model lipid membrane and structural and chemical characteristics of CPPs in order to obtain a biophysical classification that predicts the behavior of CPP-cargo molecules in cell systems. Indeed, the binding with cell membrane is one of the primary step in the interaction of CPPs with cells, and consequently the studies on model membrane could become important for understanding peptide-membrane interaction on a molecular level, explaining how CPPs may translocate a membrane without destroying it and how this interactions come into play in shuttling CPPs via different routes with different efficiency. We analyzed by CD and fluorescence spectroscopies the binding properties of six different CPPs (kFGF, Nle54-Antp and Tat derived peptides, and oligoarginine peptides containing 6, 8 or 10 residues in absence or presence of the same cargo peptide (the 392-401pTyr396 fragment of HS1 protein. The phospholipid binding properties were correlated to the conformational and chemical characteristics of peptides, as well as to the cell penetrating properties of the CPP-cargo conjugates. Results show that even if certain physico-chemical properties (conformation, positive charge govern CPP capability to interact with the model membrane, these cannot fully explain cell-permeability properties.

  15. 19 CFR 122.117 - Requirements for transit air cargo transport.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Requirements for transit air cargo transport. 122... Requirements for transit air cargo transport. (a) Transportation—(1) Port to port. Transit air cargo may be... cargo, a receipt shall be given. The receipt shall be made by the airline responsible for transport...

  16. 46 CFR 153.436 - Heat transfer fluids: compatibility with cargo.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Heat transfer fluids: compatibility with cargo. 153.436... Equipment Cargo Temperature Control Systems § 153.436 Heat transfer fluids: compatibility with cargo. A heat transfer fluid separated from the cargo by only one wall (for example, the heat transfer fluid in a...

  17. 46 CFR 153.371 - Minimum relief valve setting for refrigerated cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Minimum relief valve setting for refrigerated cargo... Design and Equipment Cargo Venting Systems § 153.371 Minimum relief valve setting for refrigerated cargo tanks. The relief valve setting for a containment system that carries a refrigerated cargo must at...

  18. 46 CFR 153.438 - Cargo pressure or temperature alarms required.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pressure or temperature alarms required. 153.438... Equipment Cargo Temperature Control Systems § 153.438 Cargo pressure or temperature alarms required. (a... vapor pressure described in § 153.371(b); or (2) An alarm that operates when the cargo's...

  19. 46 CFR 154.707 - Cargo boil-off as fuel: Ventilation.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo boil-off as fuel: Ventilation. 154.707 Section 154.707 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES... Equipment Cargo Pressure and Temperature Control § 154.707 Cargo boil-off as fuel: Ventilation. (a)...

  20. 46 CFR 98.25-55 - Cargo piping.

    Science.gov (United States)

    2010-10-01

    ... piping by means of seamless steel pipe expansion bends. Special consideration will be given for packless... Cargo piping. (a) Piping shall be of seamless steel meeting the requirements of § 56.60-1 of...

  1. 46 CFR 98.25-80 - Cargo hose.

    Science.gov (United States)

    2010-10-01

    ... rubber or other hose material acceptable to the Commandant, shall be fitted to the liquid or vapor lines... valve. (d) Before being placed in service, each new cargo hose, with all necessary fittings...

  2. Stochastic modeling of cargo transport by teams of molecular motors

    CERN Document Server

    Klein, Sarah; Santen, Ludger

    2014-01-01

    Many different types of cellular cargos are transported bidirectionally along microtubules by teams of molecular motors. The motion of this cargo-motors system has been experimentally characterized in vivo as processive with rather persistent directionality. Different theoretical approaches have been suggested in order to explore the origin of this kind of motion. An effective theoretical approach, introduced by M\\"uller et al., describes the cargo dynamics as a tug-of-war between different kinds of motors. An alternative approach has been suggested recently by Kunwar et al., who considered the coupling between motor and cargo in more detail. Based on this framework we introduce a model considering single motor positions which we propagate in continuous time. Furthermore, we analyze the possible influence of the discrete time update schemes used in previous publications on the system's dynamic.

  3. Multi-Purpose Logistics Module (MPLM) Cargo Heat Exchanger

    Science.gov (United States)

    Zampiceni, John J.; Harper, Lon T.

    2002-01-01

    This paper describes the New Shuttle Orbiter's Multi- Purpose Logistics Modulo (MPLM) Cargo Heat Exchanger (HX) and associated MPLM cooling system. This paper presents Heat Exchanger (HX) design and performance characteristics of the system.

  4. 33 CFR 157.134 - Cargo tank drainage.

    Science.gov (United States)

    2010-07-01

    ...) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.134 Cargo...

  5. Aggregating Secondary Source Data for Air Cargo Prescreening Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose to improve the effectiveness of air cargo prescreening by enabling background knowledge about companies and products to be used for threat assessment....

  6. The Cargo Tray Industry in India – A Market Research

    OpenAIRE

    Eriksson, Martin; Ohlsson, Tobias

    2010-01-01

    Title: The Cargo Tray Industry in India – A Market Research Authors: Martin Eriksson and Tobias Ohlsson Supervisor: Cecilia Lindh University: University of MälardalenDepartment: School of Sustainable Development of Society and TechnologyCourse: Bachelor thesis in Business Administration, 15 credits Research question: Is the Indian cargo tray industry a promising place for Autoform to make futureinvestments? Target group: The primary target is Autoform. The secondary target group is other cara...

  7. CARGO INSURANCE. TOTAL LOSS UNDER NORWEGIAN, ENGLISH AND RUSSIAN LAW

    OpenAIRE

    Evstigneeva, Anastasia

    2015-01-01

    This Master Thesis was created because of great personal interest towards marine insurance as such ans cargo insurance in particular. The purpose of this work is to give a proper overview of three legal fields - Norwegian, English and Russian Law. Cargo insurance is of no doubts tremendously important nowadays. How often do people consider where the goods they use daily are coming from? Some tea from India, a new phone form Japan, household appliances from Germany. All these goods were made l...

  8. Three-dimensional container and cargo inspection system

    Science.gov (United States)

    Tumer, Tumay O.; Su, Chih-Wu; Baritelle, J.; Rhoton, B.

    1997-02-01

    A fusion of two independent but complementary three- dimensional imaging techniques is proposed for detecting drugs in containers, cargo, mail and luggage. The containers, cargo, mail and/or luggage are scanned using a combined neutron and gamma ray source. A detector that can detect both neutrons and gamma rays is used to produce three dimensional images from both signals. The two images will be combined and analyzed by a fast host computer to detect drugs that may be concealed in the container, cargo and/or luggage. The two independent signatures from both neutrons and gamma rays, when analyzed simultaneously, may help determine the type of concealed material inside the containers. Containers, cargo and luggage are filled with a large variety of materials. Imaging them only in two dimensions may result in a poor contraband detection probability as different materials may shield each other. Therefore, a true three-dimensional imaging system is proposed, where the individual items inside the container or cargo can be resolved. This is expected to lead to reliable identification of the drugs even in small quantities. Such a system will also pinpoint the location of the suspected item and help expedite inspection by law enforcement agents. The proposed detection system produces two complementary three- dimensional images of the containers, cargo and/or luggage. These images are combined and analyzed by a specially developed algorithm to identify and locate the contraband automatically.

  9. Integrated nanotechnology platform for tumor-targeted multimodal imaging and therapeutic cargo release.

    Science.gov (United States)

    Hosoya, Hitomi; Dobroff, Andrey S; Driessen, Wouter H P; Cristini, Vittorio; Brinker, Lina M; Staquicini, Fernanda I; Cardó-Vila, Marina; D'Angelo, Sara; Ferrara, Fortunato; Proneth, Bettina; Lin, Yu-Shen; Dunphy, Darren R; Dogra, Prashant; Melancon, Marites P; Stafford, R Jason; Miyazono, Kohei; Gelovani, Juri G; Kataoka, Kazunori; Brinker, C Jeffrey; Sidman, Richard L; Arap, Wadih; Pasqualini, Renata

    2016-02-16

    A major challenge of targeted molecular imaging and drug delivery in cancer is establishing a functional combination of ligand-directed cargo with a triggered release system. Here we develop a hydrogel-based nanotechnology platform that integrates tumor targeting, photon-to-heat conversion, and triggered drug delivery within a single nanostructure to enable multimodal imaging and controlled release of therapeutic cargo. In proof-of-concept experiments, we show a broad range of ligand peptide-based applications with phage particles, heat-sensitive liposomes, or mesoporous silica nanoparticles that self-assemble into a hydrogel for tumor-targeted drug delivery. Because nanoparticles pack densely within the nanocarrier, their surface plasmon resonance shifts to near-infrared, thereby enabling a laser-mediated photothermal mechanism of cargo release. We demonstrate both noninvasive imaging and targeted drug delivery in preclinical mouse models of breast and prostate cancer. Finally, we applied mathematical modeling to predict and confirm tumor targeting and drug delivery. These results are meaningful steps toward the design and initial translation of an enabling nanotechnology platform with potential for broad clinical applications. PMID:26839407

  10. Spectroscopic neutron radiography for a cargo scanning system

    Science.gov (United States)

    Rahon, Jill; Danagoulian, Areg; MacDonald, Thomas D.; Hartwig, Zachary S.; Lanza, Richard C.

    2016-06-01

    Detection of cross-border smuggling of illicit materials and contraband is a challenge that requires rapid, low-dose, and efficient radiographic technology. The work we describe here is derived from a technique which uses monoenergetic gamma rays from low energy nuclear reactions, such as 11B(d,nγ)12C, to perform radiographic analysis of shipping containers. Transmission ratios of multiple monoenergetic gamma lines resulting from several gamma producing nuclear reactions can be employed to detect materials of high atomic number (Z), the details of which will be described in a separate paper. Inherent in this particular nuclear reaction is the production of fast neutrons which could enable neutron radiography and further characterization of the effective-Z of the cargo, especially within the range of lower Z. Previous research efforts focused on the use of total neutron counts in combination with X-ray radiography to characterize the hydrogenous content of the cargo. We present a technique of performing transmitted neutron spectral analysis to reconstruct the effective Z and potentially the density of the cargo. This is made possible by the large differences in the energy dependence of neutron scattering cross-sections between hydrogenous materials and those of higher Z. These dependencies result in harder transmission spectra for hydrogenous cargoes than those of non-hydrogenous cargoes. Such observed differences can then be used to classify the cargo based on its hydrogenous content. The studies presented in this paper demonstrate that such techniques are feasible and can provide a contribution to cargo security, especially when used in concert with gamma radiography.

  11. 46 CFR 151.05-2 - Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or...

    Science.gov (United States)

    2010-10-01

    ... benzene and benzene containing cargoes, or butyl acrylate cargoes. 151.05-2 Section 151.05-2 Shipping... Compliance with requirements for tank barges carrying benzene and benzene containing cargoes, or butyl acrylate cargoes. A tank barge certificated to carry benzene and benzene containing cargoes or...

  12. Simulation Of A Photofission-Based Cargo Interrogation System

    Science.gov (United States)

    King, Michael; Gozani, Tsahi; Stevenson, John; Shaw, Timothy

    2011-06-01

    A comprehensive model has been developed to characterize and optimize the detection of Bremsstrahlung x-ray induced fission signatures from nuclear materials hidden in cargo containers. An effective active interrogation system should not only induce a large number of fission events but also efficiently detect their signatures. The proposed scanning system utilizes a 9-MV commercially available linear accelerator and the detection of strong fission signals i.e. delayed gamma rays and prompt neutrons. Because the scanning system is complex and the cargo containers are large and often highly attenuating, the simulation method segments the model into several physical steps, representing each change of radiation particle. Each approximation is carried-out separately, resulting in a major reduction in computational time and a significant improvement in tally statistics. The model investigates the effect on the fission rate and detection rate by various cargo types, densities and distributions. Hydrogenous and metallic cargos, homogeneous and heterogeneous, as well as various locations of the nuclear material inside the cargo container were studied. We will show that for the photofission-based interrogation system simulation, the final results are not only in good agreement with a full, single-step simulation but also with experimental results, further validating the full-system simulation.

  13. Simulation Of A Photofission-Based Cargo Interrogation System

    International Nuclear Information System (INIS)

    A comprehensive model has been developed to characterize and optimize the detection of Bremsstrahlung x-ray induced fission signatures from nuclear materials hidden in cargo containers. An effective active interrogation system should not only induce a large number of fission events but also efficiently detect their signatures. The proposed scanning system utilizes a 9-MV commercially available linear accelerator and the detection of strong fission signals i.e. delayed gamma rays and prompt neutrons. Because the scanning system is complex and the cargo containers are large and often highly attenuating, the simulation method segments the model into several physical steps, representing each change of radiation particle. Each approximation is carried-out separately, resulting in a major reduction in computational time and a significant improvement in tally statistics. The model investigates the effect on the fission rate and detection rate by various cargo types, densities and distributions. Hydrogenous and metallic cargos, homogeneous and heterogeneous, as well as various locations of the nuclear material inside the cargo container were studied. We will show that for the photofission-based interrogation system simulation, the final results are not only in good agreement with a full, single-step simulation but also with experimental results, further validating the full-system simulation.

  14. Stokes Efficiency of Molecular Motor-Cargo Systems

    Directory of Open Access Journals (Sweden)

    Hong Zhou

    2008-05-01

    Full Text Available A molecular motor utilizes chemical free energy to generate a unidirectional motion through the viscous fluid. In many experimental settings and biological settings, a molecular motor is elastically linked to a cargo. The stochastic motion of a molecular motor-cargo system is governed by a set of Langevin equations, each corresponding to an individual chemical occupancy state. The change of chemical occupancy state is modeled by a continuous time discrete space Markov process. The probability density of a motor-cargo system is governed by a two-dimensional Fokker-Planck equation. The operation of a molecular motor is dominated by high viscous friction and large thermal fluctuations from surrounding fluid. The instantaneous velocity of a molecular motor is highly stochastic: the past velocity is quickly damped by the viscous friction and the new velocity is quickly excited by bombardments of surrounding fluid molecules. Thus, the theory for macroscopic motors should not be applied directly to molecular motors without close examination. In particular, a molecular motor behaves differently working against a viscous drag than working against a conservative force. The Stokes efficiency was introduced to measure how efficiently a motor uses chemical free energy to drive against viscous drag. For a motor without cargo, it was proved that the Stokes efficiency is bounded by 100% [H. Wang and G. Oster, (2002]. Here, we present a proof for the general motor-cargo system.

  15. Optimisation of key performance measures in air cargo demand management

    Directory of Open Access Journals (Sweden)

    Alexander May

    2014-03-01

    Full Text Available This article sought to facilitate the optimisation of key performance measures utilised for demand management in air cargo operations. The focus was on the Revenue Management team at Virgin Atlantic Cargo and a fuzzy group decision-making method was used. Utilising intelligent fuzzy multi-criteria methods, the authors generated a ranking order of ten key outcome-based performance indicators for Virgin Atlantic air cargo Revenue Management. The result of this industry-driven study showed that for Air Cargo Revenue Management, ‘Network Optimisation’ represents a critical outcome-based performance indicator. This collaborative study contributes to existing logistics management literature, especially in the area of Revenue Management, and it seeks to enhance Revenue Management practice. It also provides a platform for Air Cargo operators seeking to improve reliability values for their key performance indicators as a means of enhancing operational monitoring power.

  16. Effect of cargo size and shape on the transport efficiency of the bacterial Tat translocase.

    Science.gov (United States)

    Whitaker, Neal; Bageshwar, Umesh; Musser, Siegfried M

    2013-04-01

    The Tat machinery translocates fully-folded and oligomeric substrates. The passage of large, bulky cargos across an ion-tight membrane suggests the need to match pore and cargo size, and therefore that Tat transport efficiency may depend on both cargo size and shape. A series of cargos of different sizes and shapes were generated using the natural Tat substrate pre-SufI as a base. Four (of 17) cargos transported with significant (>20% of wild-type) efficiencies. These results indicate that cargo size and shape significantly influence Tat transportability. PMID:23422074

  17. Detection of Shielded Nuclear Material in a Cargo Container

    International Nuclear Information System (INIS)

    The Idaho National Laboratory, along with Los Alamos National Laboratory and the Idaho State University's Idaho Accelerator Center, are developing electron accelerator-based, photonuclear inspection technologies for the detection of shielded nuclear material within air-, rail-, and especially, maritime-cargo transportation containers. This paper describes a developing prototypical cargo container inspection system utilizing the Pulsed Photonuclear Assessment (PPA) technology, incorporates interchangeable, well-defined, contraband shielding structures (i.e., ''calibration'' pallets) providing realistic detection data for induced radiation signatures from smuggled nuclear material, and provides various shielded nuclear material detection results. Using a 4.8-kg quantity of depleted uranium, neutron and gamma-ray detection responses are presented for well-defined shielded and unshielded configurations evaluated in a selected cargo container inspection configuration

  18. Detection of shielded nuclear material in a cargo container

    Science.gov (United States)

    Jones, James L.; Norman, Daren R.; Haskell, Kevin J.; Sterbentz, James W.; Yoon, Woo Y.; Watson, Scott M.; Johnson, James T.; Zabriskie, John M.; Bennett, Brion D.; Watson, Richard W.; Moss, Cavin E.; Frank Harmon, J.

    2006-06-01

    The Idaho National Laboratory, along with Los Alamos National Laboratory and the Idaho State University's Idaho Accelerator Center, are developing electron accelerator-based, photonuclear inspection technologies for the detection of shielded nuclear material within air-, rail-, and especially, maritime-cargo transportation containers. This paper describes a developing prototypical cargo container inspection system utilizing the Pulsed Photonuclear Assessment (PPA) technology, incorporates interchangeable, well-defined, contraband shielding structures (i.e., "calibration" pallets) providing realistic detection data for induced radiation signatures from smuggled nuclear material, and provides various shielded nuclear material detection results. Using a 4.8-kg quantity of depleted uranium, neutron and gamma-ray detection responses are presented for well-defined shielded and unshielded configurations evaluated in a selected cargo container inspection configuration.

  19. Detection of shielded nuclear material in a cargo container

    International Nuclear Information System (INIS)

    The Idaho National Laboratory, along with Los Alamos National Laboratory and the Idaho State University's Idaho Accelerator Center, are developing electron accelerator-based, photonuclear inspection technologies for the detection of shielded nuclear material within air-, rail-, and especially, maritime-cargo transportation containers. This paper describes a developing prototypical cargo container inspection system utilizing the Pulsed Photonuclear Assessment (PPA) technology, incorporates interchangeable, well-defined, contraband shielding structures (i.e., 'calibration' pallets) providing realistic detection data for induced radiation signatures from smuggled nuclear material, and provides various shielded nuclear material detection results. Using a 4.8-kg quantity of depleted uranium, neutron and gamma-ray detection responses are presented for well-defined shielded and unshielded configurations evaluated in a selected cargo container inspection configuration

  20. Multipurpose Cargo Transfer Bags fro Reducing Exploration Mission Logistics

    Science.gov (United States)

    Baccus, Shelley; Broyan, James Lee, Jr.; Borrego, Melissa

    2016-01-01

    The Logistics Reduction (LR) project within the Advanced Exploration Systems (AES) division is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag (CTB), the common logistics carrying bag for the International Space Station (ISS). After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unfolded into a flat panel for reuse. Concepts and potential benefits for various MCTB applications will be discussed including partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing. Acoustic MCTBs are currently in use on ISS to reduce the noise generated by the T2 treadmill, which reaches the hazard limit at high speeds. The development of the AMCTB included identification of keep out zones, acoustic properties, deployment considerations, and structural testing. Features developed for these considerations are applicable to MCTBs for all crew outfitting applications.

  1. Reducing Mission Logistics with Multipurpose Cargo Transfer Bags

    Science.gov (United States)

    Baccus, Shelley; Broyan, James Lee, Jr.; Borrego, Melissa

    2016-01-01

    The Logistics Reduction (LR) project within Advanced Exploration Systems (AES) is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag (CTB), the common logistics carrying bag for the International Space Station (ISS). After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unfolded into a flat panel for reuse. Concepts and potential benefits for various MCTB applications will be discussed including partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing. Acoustic MCTBs are currently in use on ISS to reduce the noise generated by the T2 treadmill, which reaches the hazard limit at high speeds. The development of the AMCTB included identification of keep-out zones, acoustic properties, deployment considerations, and structural testing. Features developed for these considerations are applicable to MCTBs for all crew outfitting applications.

  2. Optimizing an undulating magnetic microswimmer for cargo towing

    Science.gov (United States)

    Gutman, Emiliya; Or, Yizhar

    2016-06-01

    One of the promising capabilities of magnetic microswimmers is towing a cargo, which can be used for targeted drug delivery or performing tissue biopsy. A key question is what should be the optimal size ratio between the cargo and the swimmer's flexible tail. This question is addressed here for the simplest theoretical model of a magnetic microswimmer undergoing planar undulations—a spherical load connected by a torsion spring to a rigid slender link. The swimmer's dynamic is formulated and leading-order expressions for its motion are obtained explicitly under small-amplitude approximation. Optimal combinations of magnetic actuation frequency, torsion stiffness, and tail length for maximizing displacement, average speed, or energetic efficiency are obtained. The theoretical results are compared with reported experiments in several types of cargo-towing magnetic microswimmers.

  3. Minimization of Load Oscillations in Travelling Mechanisms of Cranes

    Directory of Open Access Journals (Sweden)

    Kamal Khandakji

    2005-01-01

    Full Text Available The paper presents an analysis of suspended load (cargo oscillations in traveling mechanisms of cranes. A special control algorithm has been developed in order to get minimum cargo oscillations in minimum transient time. Mathematical model simulating the effect of the suggested control algorithm on cargo oscillations has been developed. Two power electronic circuits applicable for AC drives with three-phase induction motors are suggested to obtain the required motion algorithm.

  4. Experimental ship fire measurements with simulated radioactive cargo

    International Nuclear Information System (INIS)

    Results from a series of eight test fires ranging in size from 2.2 to 18.8 MW conducted aboard the Coast Guard fire test ship Mayo Lykes at Mobile, Alabama are presented and discussed. Tests aboard the break bulk type cargo ship consisted of heptane spray fires simulating engine room and galley fires, wood crib fires simulating cargo hold fires, and pool fires staged for comparison to land based regulatory fire results. Primary instrumentation for the tests consisted of two pipe calorimeters that simulated a typical package shape for radioactive materials packages

  5. SHIP AND CARGO MORTGAGE, TWO DIFFERENT LEGAL VIEWPOINTS

    Directory of Open Access Journals (Sweden)

    Mohammad Abouata

    2015-05-01

    Full Text Available Although the subject-matter of cargo and ship mortgage are similar (providing financial security for creditors,the regulations of these contracts, differ from each other essentially with regard to the conditionality of taking possession in concluding the contract, the possibility of endorsement and cession in mortgage document, the creditor s kind of real right ,the preference of prior or latter creditor in vindication of right and the effect of mortgage loss on the contract and the possibility of recovery of claim. Moreover the cargo mortgage regulations are, in some regards and aspects, contrary to general legal rules and hence subject to criticism.

  6. Column generation approaches to ship scheduling with flexible cargo sizes

    DEFF Research Database (Denmark)

    Brønmo, Geir; Nygreen, Bjørn; Lysgaard, Jens

    cargo sizes to the column generation framework is not straightforward, and we handle the flexible cargo sizes heuristically when solving the subproblems. This leads to convergence issues in the branch-and-price search tree, and the optimal solution cannot be guaranteed. Hence we have introduced a method...... that generates an upper bound on the optimal objective. We have compared our method with an a priori column generation approach, and our computational experiments on real world cases show that the Dantzig-Wolfe approach is faster than the a priori generation of columns, and we are able to deal with...

  7. Reconstitution of mitochondria derived vesicle formation demonstrates selective enrichment of oxidized cargo.

    Directory of Open Access Journals (Sweden)

    Vincent Soubannier

    Full Text Available The mechanisms that ensure the removal of damaged mitochondrial proteins and lipids are critical for the health of the cell, and errors in these pathways are implicated in numerous degenerative diseases. We recently uncovered a new pathway for the selective removal of proteins mediated by mitochondrial derived vesicular carriers (MDVs that transit to the lysosome. However, it was not determined whether these vesicles were selectively enriched for oxidized, or damaged proteins, and the extent to which the complexes of the electron transport chain and the mtDNA-containing nucloids may have been incorporated. In this study, we have developed a cell-free mitochondrial budding reaction in vitro in order to better dissect the pathway. Our data confirm that MDVs are stimulated upon various forms of mitochondrial stress, and the vesicles incorporated quantitative amounts of cargo, whose identity depended upon the nature of the stress. Under the conditions examined, MDVs did not incorporate complexes I and V, nor were any nucleoids present, demonstrating the specificity of cargo incorporation. Stress-induced MDVs are selectively enriched for oxidized proteins, suggesting that conformational changes induced by oxidation may initiate their incorporation into the vesicles. Ultrastructural analyses of MDVs isolated on sucrose flotation gradients revealed the formation of both single and double membranes vesicles of unique densities and uniform diameter. This work provides a framework for a reductionist approach towards a detailed examination of the mechanisms of MDV formation and cargo incorporation, and supports the emerging concept that MDVs are critical contributors to mitochondrial quality control.

  8. 46 CFR 308.512 - Declaration of shipments under Open Cargo Policy.

    Science.gov (United States)

    2010-10-01

    ... OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.512... shipments and a closing report for all outward shipments, and pay the premium and fees, for all...

  9. 49 CFR 178.348 - Specification DOT 412; cargo tank motor vehicle.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 412; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.348 Specification DOT 412; cargo tank motor vehicle....

  10. 49 CFR 178.346 - Specification DOT 406; cargo tank motor vehicle.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 406; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.346 Specification DOT 406; cargo tank motor vehicle....

  11. 49 CFR 178.347 - Specification DOT 407; cargo tank motor vehicle.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 407; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.347 Specification DOT 407; cargo tank motor vehicle....

  12. 中国-东盟自由贸易区大部分货物贸易2010年零关税%Zero tariff expected for most cargo trade in China-ASEAN free trade zone by 2010

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The negotiation committees of China and the Association of Southeast Asian Nations (ASEAN) have reached agreement on the most important prat-agreement on cargo trade, according to the Ministry of Commerce spokesman Chong Quan. The two sides decided to sign two agreements on cargo trade and dispute resolution mechanism of the China-ASEAN free trade zone duringthe China-ASEAN leaders' meeting in November 2004.

  13. STATISTICAL ANALYSIS OF THE EFFECTIVENESS OF THE ACCOUNTS RECEIVABLE MANAGEMENT ON THE CARGO MOTOR TRANSPORTATION ENTERPRISES

    OpenAIRE

    Kylnytsvska, Y.; Gluhova, S.

    2014-01-01

    In the article the efficiency of accounts receivable management on cargo motor transportation enterprises is analyzed using the method of statistical analysis, namely middle descriptions of variation row of account receivable of cargo motor transportation enterprises are analysed; the analysis of unevenness of distributing of the account receivable of cargo motor transportation enterprises is conducted; the degree of dirigibility of conduct of debtors of cargo motor transportation enterprises...

  14. 46 CFR 154.705 - Cargo boil-off as fuel: General.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo boil-off as fuel: General. 154.705 Section 154.705... Pressure and Temperature Control § 154.705 Cargo boil-off as fuel: General. (a) Each cargo boil-off fuel system under § 154.703(c) must meet §§ 154.706 through 154.709. (b) The piping in the cargo boil-off...

  15. Safety evaluation for packaging (Onsite) transport of LSA-II liquids in MC-312 cargo tanks

    International Nuclear Information System (INIS)

    This safety evaluation for packaging authorizes the onsite transfer of bulk LSA-II radioactive liquids in the 222-S Laboratory Cargo Tank and Liquid Effluent Treatment Facility Cargo Tanks (which are U.S. Department of Transportation MC-312 specification cargo tanks) from their operating facilities to tank farm facilities

  16. 46 CFR 154.706 - Cargo boil-off as fuel: Fuel lines.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo boil-off as fuel: Fuel lines. 154.706 Section 154... Equipment Cargo Pressure and Temperature Control § 154.706 Cargo boil-off as fuel: Fuel lines. (a) Gas fuel lines must not pass through accommodation, service, or control spaces. Each gas fuel line...

  17. 46 CFR 308.549 - Application for appointment of Cargo Underwriting Agent, Form MA-319.

    Science.gov (United States)

    2010-10-01

    ... EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Iv-General § 308.549 Application for appointment of Cargo Underwriting Agent, Form MA-319. Any domestic insurance company authorized to do a marine insurance business in any State of the United States may apply for appointment as a Cargo Underwriting...

  18. 46 CFR 154.650 - Cargo tank and process pressure vessel welding.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank and process pressure vessel welding. 154.650... Equipment Construction § 154.650 Cargo tank and process pressure vessel welding. (a) Cargo tank and process pressure vessel welding must meet Subpart 54.05 and Part 57 of this chapter. (b) Welding consumables...

  19. 46 CFR 154.701 - Cargo pressure and temperature control: General.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pressure and temperature control: General. 154.701 Section 154.701 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS... Equipment Cargo Pressure and Temperature Control § 154.701 Cargo pressure and temperature control:...

  20. 46 CFR 153.370 - Minimum relief valve setting for ambient temperature cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Minimum relief valve setting for ambient temperature... temperature cargo tanks. The relief valve setting for a containment system that carries a cargo at ambient temperature must at least equal the cargo's vapor pressure at 46 °C (approx. 115 °F)....

  1. 46 CFR 91.60-5 - Cargo Ship Safety Construction Certificate.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate. 91.60-5..., 1974 § 91.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued...

  2. 46 CFR 189.60-5 - Cargo Ship Safety Construction Certificate.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate. 189.60-5..., 1974 § 189.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued...

  3. 7 CFR 1493.3 - Restrictions on programs and cargo preference statement.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Restrictions on programs and cargo preference... Restrictions on programs and cargo preference statement. (a) Restrictions on use of credit guarantees. (1... service the debt associated with such sales. (b) Cargo preference laws. The provisions of the...

  4. 46 CFR 154.709 - Cargo boil-off as fuel: Gas detection equipment.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo boil-off as fuel: Gas detection equipment. 154.709 Section 154.709 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS... Equipment Cargo Pressure and Temperature Control § 154.709 Cargo boil-off as fuel: Gas detection...

  5. 46 CFR 154.708 - Cargo boil-off as fuel: Valves.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo boil-off as fuel: Valves. 154.708 Section 154.708 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY... Pressure and Temperature Control § 154.708 Cargo boil-off as fuel: Valves. (a) Gas fuel lines to the...

  6. Transportation of Nanoscale Cargoes by Myosin Propelled Actin Filaments

    NARCIS (Netherlands)

    Persson, Malin; Gullberg, Maria; Tolf, Conny; Lindberg, A. Michael; Mansson, Alf; Kocer, Armagan

    2013-01-01

    Myosin II propelled actin filaments move ten times faster than kinesin driven microtubules and are thus attractive candidates as cargo-transporting shuttles in motor driven lab-on-a-chip devices. In addition, actomyosin-based transportation of nanoparticles is useful in various fundamental studies.

  7. 14 CFR 29.787 - Cargo and baggage compartments.

    Science.gov (United States)

    2010-01-01

    ... emergency landing conditions of § 29.561. (b) There must be means to prevent the contents of any compartment...) Under the emergency landing conditions of § 29.561, cargo and baggage compartments must— (1) Be... any of the escape facilities provided for use after an emergency landing; or (2) Have...

  8. 14 CFR 27.787 - Cargo and baggage compartments.

    Science.gov (United States)

    2010-01-01

    ... emergency landing conditions of § 27.561. (b) There must be means to prevent the contents of any compartment...) Under the emergency landing conditions of § 27.561, cargo and baggage compartments must— (1) Be... any of the escape facilities provided for use after an emergency landing; or (2) Have...

  9. 48 CFR 752.228-9 - Cargo insurance.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Cargo insurance. 752.228-9... insurance. As prescribed in 728.313(a), the following preface is to be used preceding the text of the clause at FAR 52.228-9: Preface: To the extent that marine insurance is necessary or appropriate under...

  10. Combining technologies - radiography and neutron based - for cargo security applications

    International Nuclear Information System (INIS)

    Inspection of air and sea cargo has traditionally been done by X-ray systems of various energies relying on operators to analyze images looking for anomalies in the image of cargo that may signify a threat. This has shown only limited success in detecting explosives and other threats, which do not have any distinctive shapes. OSI Systems, through its subsidiaries Rapiscan and Ancore, has combined high-energy x-ray radiography with thermal neutron analysis (TNA) to create the combined system-''TNX''. The system provides automatic material specific detection of bulk threat items, like explosives, while furnishing the operator with a high-resolution image for weapons detection and also to identify anomalies for the TNA to inspect. Similarly the Pulsed Fast Neutron Analysis (PFNA) can be combined with high-energy x-ray to create a ''FNX'' system for both air and sea cargo applications. This enables the operator obtain a three dimensional image of the material composition of the cargo under inspection and remove the clutter from the image leaving only the potentially hazardous material(s) automatically while viewing a high resolution image for manifest verification and weapons. The current status of the technology will be discussed and data be presented

  11. 46 CFR 151.50-5 - Cargoes having toxic properties.

    Science.gov (United States)

    2010-10-01

    ...) Gravity type cargo tanks shall be fitted with an approved pressure-vacuum relief valve of not less than 21... or pressure vacuum relief valve shall be directed in such a manner as to not impinge on another tank... from the safety or pressure vacuum relief valve as a result of a fire or other casualty. In...

  12. 46 CFR 151.20-5 - Cargo system valving requirements.

    Science.gov (United States)

    2010-10-01

    ... will cause the quick closing shutoff valves to close in case of fire. Quick closing shutoff valves... penetrations are not applicable to nozzles at which pressure vacuum or safety relief valves are fitted. (a... conditions, every cargo piping system shall be provided with one of the following sets of control valves...

  13. 29 CFR 1918.85 - Containerized cargo operations.

    Science.gov (United States)

    2010-07-01

    ... required in paragraph (a)(3) of this section, or it exceeds the capacity of the lifting appliance. (d... rated capacity. (D) The lift angle is at 80 to 90 degrees; (E) The distance between the lifting beam and... maximum cargo weight, in pounds. (b) Container weight. No container shall be hoisted by any...

  14. 46 CFR 13.309 - Eligibility: Cargo course.

    Science.gov (United States)

    2010-10-01

    ... TANKERMEN Requirements for âTankerman-PIC (Barge)â Endorsement § 13.309 Eligibility: Cargo course. Each applicant for an original “Tankerman-PIC (Barge)” endorsement shall present a certificate of completion from a course in DL or LG appropriate for tank barges and for Tankerman-PIC or Tankerman-PIC (Barge),...

  15. Mars Hybrid Propulsion System Trajectory Analysis. Part II; Cargo Missions

    Science.gov (United States)

    Chai, Patrick R.; Merrill, Raymond G.; Qu, Min

    2015-01-01

    NASA's Human Spaceflight Architecture Team is developing a reusable hybrid transportation architecture in which both chemical and electric propulsion systems are used to send crew and cargo to Mars destinations such as Phobos, Deimos, the surface of Mars, and other orbits around Mars. By combining chemical and electrical propulsion into a single spaceship and applying each where it is more effective, the hybrid architecture enables a series of Mars trajectories that are more fuel-efficient than an all chemical architecture without significant increases in flight times. This paper shows the feasibility of the hybrid transportation architecture to pre-deploy cargo to Mars and Phobos in support of the Evolvable Mars Campaign crew missions. The analysis shows that the hybrid propulsion stage is able to deliver all of the current manifested payload to Phobos and Mars through the first three crew missions. The conjunction class trajectory also allows the hybrid propulsion stage to return to Earth in a timely fashion so it can be reused for additional cargo deployment. The 1,100 days total trip time allows the hybrid propulsion stage to deliver cargo to Mars every other Earth-Mars transit opportunity. For the first two Mars surface mission in the Evolvable Mars Campaign, the short trip time allows the hybrid propulsion stage to be reused for three round-trip journeys to Mars, which matches the hybrid propulsion stage's designed lifetime for three round-trip crew missions to the Martian sphere of influence.

  16. 46 CFR 148.01-7 - Permitted cargoes.

    Science.gov (United States)

    2010-10-01

    ... heating and ignition. Note: Definitions of hazard classes of hazardous materials are found in 49 CFR 171.8 and 49 CFR 173.500. (b) A mixture or blend of two or more cargoes, one or more of which is listed in... Flammable solid Contact with water may cause self heating. Coconut meal pellets (or copra...

  17. 19 CFR 4.62 - Accounting for inward cargo.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Accounting for inward cargo. 4.62 Section 4.62 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY VESSELS IN FOREIGN AND DOMESTIC TRADES Foreign Clearances § 4.62 Accounting for inward...

  18. Column generation approaches to ship scheduling with flexible cargo sizes

    DEFF Research Database (Denmark)

    Brønmo, Geir; Nygreen, Bjørn; Lysgaard, Jens

    2010-01-01

    consequences for the times used in the ports because both the loading and unloading times depend on the cargo sizes. We found it computationally hard to find exact solutions to the subproblems, so our method does not guarantee to find the optimum over all solutions. To be able to say something about how good...

  19. Integrated cargo routing and ship scheduling in liner shipping

    DEFF Research Database (Denmark)

    Kjeldsen, Karina Hjortshøj

    The problem consists of creating routes and schedules for a heterogeneous fleet of ships while determining the cargo routing and the speed for all relevant port pair/ship combinations. Transshipment is allowed in ports with transshipment capabilities. The service frequency is fixed at one week...

  20. 33 CFR 157.132 - Cargo tanks: Hydrocarbon vapor emissions.

    Science.gov (United States)

    2010-07-01

    ... SECURITY (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.132 Cargo tanks: Hydrocarbon vapor emissions. Each tank vessel having a COW system under §...

  1. An exact approach for studying cargo transport by an ensemble of molecular motors

    International Nuclear Information System (INIS)

    Intracellular transport is crucial for many cellular processes where a large fraction of the cargo is transferred by motor-proteins over a network of microtubules. Malfunctions in the transport mechanism underlie a number of medical maladies. Existing methods for studying how motor-proteins coordinate the transfer of a shared cargo over a microtubule are either analytical or are based on Monte-Carlo simulations. Approaches that yield analytical results, while providing unique insights into transport mechanism, make simplifying assumptions, where a detailed characterization of important transport modalities is difficult to reach. On the other hand, Monte-Carlo based simulations can incorporate detailed characteristics of the transport mechanism; however, the quality of the results depend on the number and quality of simulation runs used in arriving at results. Here, for example, it is difficult to simulate and study rare-events that can trigger abnormalities in transport. In this article, a semi-analytical methodology that determines the probability distribution function of motor-protein behavior in an exact manner is developed. The method utilizes a finite-dimensional projection of the underlying infinite-dimensional Markov model, which retains the Markov property, and enables the detailed and exact determination of motor configurations, from which meaningful inferences on transport characteristics of the original model can be derived. Under this novel probabilistic approach new insights about the mechanisms of action of these proteins are found, suggesting hypothesis about their behavior and driving the design and realization of new experiments. The advantages provided in accuracy and efficiency make it possible to detect rare events in the motor protein dynamics, that could otherwise pass undetected using standard simulation methods. In this respect, the model has allowed to provide a possible explanation for possible mechanisms under which motor proteins could

  2. Applications and Challenges for Use of Cell-Penetrating Peptides as Delivery Vectors for Peptide and Protein Cargos

    Directory of Open Access Journals (Sweden)

    Mie Kristensen

    2016-01-01

    Full Text Available The hydrophilic nature of peptides and proteins renders them impermeable to cell membranes. Thus, in order to successfully deliver peptide and protein-based therapeutics across the plasma membrane or epithelial and endothelial barriers, a permeation enhancing strategy must be employed. Cell-penetrating peptides (CPPs constitute a promising tool and have shown applications for peptide and protein delivery into cells as well as across various epithelia and the blood-brain barrier (BBB. CPP-mediated delivery of peptides and proteins may be pursued via covalent conjugation of the CPP to the cargo peptide or protein or via physical complexation obtained by simple bulk-mixing of the CPP with its cargo. Both approaches have their pros and cons, and which is the better choice likely relates to the physicochemical properties of the CPP and its cargo as well as the route of administration, the specific barrier and the target cell. Besides the physical barrier, a metabolic barrier must be taken into consideration when applying peptide-based delivery vectors, such as the CPPs, and stability-enhancing strategies are commonly employed to prolong the CPP half-life. The mechanisms by which CPPs translocate cell membranes are believed to involve both endocytosis and direct translocation, but are still widely investigated and discussed. The fact that multiple factors influence the mechanisms responsible for cellular CPP internalization and the lack of sensitive methods for detection of the CPP, and in some cases the cargo, further complicates the design and conduction of conclusive mechanistic studies.

  3. Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.

    Science.gov (United States)

    Pan, Ping-Yue; Marrs, Julia; Ryan, Timothy A

    2015-09-11

    A long standing question in synaptic physiology is how neurotransmitter-filled vesicles are rebuilt after exocytosis. Among the first steps in this process is the endocytic retrieval of the transmembrane proteins that are enriched in synaptic vesicles (SVs). At least six types of transmembrane proteins must be recovered, but the rules for how this multiple cargo selection is accomplished are poorly understood. Among these SV cargos is the vesicular glutamate transporter (vGlut). We show here that vGlut1 has a strong influence on the kinetics of retrieval of half of the known SV cargos and that specifically impairing the endocytosis of vGlut1 in turn slows down other SV cargos, demonstrating that cargo retrieval is a collective cargo-driven process. Finally, we demonstrate that different cargos can be retrieved in the same synapse with different kinetics, suggesting that additional post-endocytic sorting steps likely occur in the nerve terminal. PMID:26224632

  4. 46 CFR 153.490 - Cargo Record Book and Approved Procedures and Arrangements Manual: Categories A, B, C, and D.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo Record Book and Approved Procedures and... Cargo Record Book and Approved Procedures and Arrangements Manual: Categories A, B, C, and D. (a) Unless... carry NLS cargo, a ship must have— (1) If U.S., a Cargo Record Book published by the Coast Guard...

  5. The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo

    Directory of Open Access Journals (Sweden)

    Jeoffrey Schageman

    2013-01-01

    Full Text Available Exosomes are small (30–150 nm vesicles containing unique RNA and protein cargo, secreted by all cell types in culture. They are also found in abundance in body fluids including blood, saliva, and urine. At the moment, the mechanism of exosome formation, the makeup of the cargo, biological pathways, and resulting functions are incompletely understood. One of their most intriguing roles is intercellular communication—exosomes function as the messengers, delivering various effector or signaling macromolecules between specific cells. There is an exponentially growing need to dissect structure and the function of exosomes and utilize them for development of minimally invasive diagnostics and therapeutics. Critical to further our understanding of exosomes is the development of reagents, tools, and protocols for their isolation, characterization, and analysis of their RNA and protein contents. Here we describe a complete exosome workflow solution, starting from fast and efficient extraction of exosomes from cell culture media and serum to isolation of RNA followed by characterization of exosomal RNA content using qRT-PCR and next-generation sequencing techniques. Effectiveness of this workflow is exemplified by analysis of the RNA content of exosomes derived from HeLa cell culture media and human serum, using Ion Torrent PGM as a sequencing platform.

  6. Development of a Cargo Screening Process Simulator: A First Approach

    CERN Document Server

    Siebers, Peer-Olaf; Aickelin, Uwe

    2010-01-01

    The efficiency of current cargo screening processes at sea and air ports is largely unknown as few benchmarks exists against which they could be measured. Some manufacturers provide benchmarks for individual sensors but we found no benchmarks that take a holistic view of the overall screening procedures and no benchmarks that take operator variability into account. Just adding up resources and manpower used is not an effective way for assessing systems where human decision-making and operator compliance to rules play a vital role. Our aim is to develop a decision support tool (cargo-screening system simulator) that will map the right technology and manpower to the right commodity-threat combination in order to maximise detection rates. In this paper we present our ideas for developing such a system and highlight the research challenges we have identified. Then we introduce our first case study and report on the progress we have made so far.

  7. Implementation of cargo MagLev in the United States

    Energy Technology Data Exchange (ETDEWEB)

    Rose, Chris R [Los Alamos National Laboratory; Peterson, Dean E [Los Alamos National Laboratory; Leung, Eddie M [MAGTEC ENGINEERING

    2008-01-01

    Numerous studies have been completed in the United States, but no commercial MagLev systems have been deployed. Outside the U.S., MagLev continues to attract funding for research, development and implementation. A brief review of recent global developments in MagLev technology is given followed by the status of MagLev in the U.S. The paper compares the cost of existing MagLev systems with other modes of transport, notes that the near-term focus of MagLev development in the U.S. should be for cargo, and suggests that future MagLev systems should be for very high speed cargo. The Los Angeles to Port of Los Angeles corridor is suggested as a first site for implementation. The benefits of MagLev are described along with suggestions on how to obtain funding.

  8. The Application Model of Moving Objects in Cargo Delivery System

    Institute of Scientific and Technical Information of China (English)

    ZHANG Feng-li; ZHOU Ming-tian; XU Bo

    2004-01-01

    The development of spatio-temporal database systems is primarily motivated by applications which track and present mobile objects. In this paper, solutions for establishing the moving object database based on GPS/GIS environment are presented, and a data modeling of moving object is given by using Temporal logical to extent the query language, finally the application model in cargo delivery system is shown.

  9. Reject or embrace? Messengers and electric cargo bikes

    OpenAIRE

    Gruber, Johannes; Kihm, Alexander

    2015-01-01

    One of many approaches to react to the challenges faced by urban freight can be the introduction of electric cargo bikes as an environmentally friendly mode of transport for courier deliveries. Since this market consists of highly decentralized decision-making structures, it is important to characterize the individuals involved and their perceptions in order to estimate market potentials and identify barriers to market uptake. To achieve this goal, we use information from a nationwide survey ...

  10. SIMULATION OF CARGO CONTAINER INTERROGATION BY D-D NEUTRONS

    International Nuclear Information System (INIS)

    High fidelity, three-dimensional computer models based on a CAD drawing of an intermodal cargo container, representative payload objects, and detector array panels were developed to simulate the underlying physical events taking place during active interrogation. These computer models are used to assess the performance of interrogation systems with different sources and detection schemes. In this presentation, we will show that the use oversimplified models, such as analyzing homogenized payloads only, can lead to errors in determining viable approaches for interrogation

  11. SHIP AND CARGO MORTGAGE, TWO DIFFERENT LEGAL VIEWPOINTS

    OpenAIRE

    Mohammad Abouata

    2015-01-01

    Although the subject-matter of cargo and ship mortgage are similar (providing financial security for creditors),the regulations of these contracts, differ from each other essentially with regard to the conditionality of taking possession in concluding the contract, the possibility of endorsement and cession in mortgage document, the creditor s kind of real right ,the preference of prior or latter creditor in vindication of right and the effect of mortgage loss on the contract and the pos...

  12. SIMULATION OF CARGO CONTAINER INTERROGATION BY D-D NEUTRONS

    Energy Technology Data Exchange (ETDEWEB)

    Lou, Tak Pui; Antolak, Arlyn

    2007-02-15

    High fidelity, three-dimensional computer models based on a CAD drawing of an intermodal cargo container, representative payload objects, and detector array panels were developed to simulate the underlying physical events taking place during active interrogation. These computer models are used to assess the performance of interrogation systems with different sources and detection schemes. In this presentation, we will show that the use oversimplified models, such as analyzing homogenized payloads only, can lead to errors in determining viable approaches for interrogation.

  13. Hydrogel Walkers with Electro-Driven Motility for Cargo Transport

    OpenAIRE

    Chao Yang; Wei Wang; Chen Yao; Rui Xie; Xiao-Jie Ju; Zhuang Liu; Liang-Yin Chu

    2015-01-01

    In this study, soft hydrogel walkers with electro-driven motility for cargo transport have been developed via a facile mould-assisted strategy. The hydrogel walkers consisting of polyanionic poly(2-acrylamido-2-methylpropanesulfonic acid-co-acrylamide) exhibit an arc looper-like shape with two “legs” for walking. The hydrogel walkers can reversibly bend and stretch via repeated “on/off” electro-triggers in electrolyte solution. Based on such bending/stretching behaviors, the hydrogel walkers ...

  14. A Novel Multilayered RFID Tagged Cargo Integrity Assurance Scheme.

    Science.gov (United States)

    Yang, Ming Hour; Luo, Jia Ning; Lu, Shao Yong

    2015-01-01

    To minimize cargo theft during transport, mobile radio frequency identification (RFID) grouping proof methods are generally employed to ensure the integrity of entire cargo loads. However, conventional grouping proofs cannot simultaneously generate grouping proofs for a specific group of RFID tags. The most serious problem of these methods is that nonexistent tags are included in the grouping proofs because of the considerable amount of time it takes to scan a high number of tags. Thus, applying grouping proof methods in the current logistics industry is difficult. To solve this problem, this paper proposes a method for generating multilayered offline grouping proofs. The proposed method provides tag anonymity; moreover, resolving disputes between recipients and transporters over the integrity of cargo deliveries can be expedited by generating grouping proofs and automatically authenticating the consistency between the receipt proof and pick proof. The proposed method can also protect against replay attacks, multi-session attacks, and concurrency attacks. Finally, experimental results verify that, compared with other methods for generating grouping proofs, the proposed method can efficiently generate offline grouping proofs involving several parties in a supply chain using mobile RFID. PMID:26512673

  15. Diffusion-Limited Cargo Loading of an Engineered Protein Container.

    Science.gov (United States)

    Zschoche, Reinhard; Hilvert, Donald

    2015-12-30

    The engineered bacterial nanocompartment AaLS-13 is a promising artificial encapsulation system that exploits electrostatic interactions for cargo loading. In order to study its ability to take up and retain guests, a pair of fluorescent proteins was developed which allows spectroscopic determination of the extent of encapsulation by Förster resonance energy transfer (FRET). The encapsulation process is generally complete within a second, suggesting low energetic barriers for proteins to cross the capsid shell. Formation of intermediate aggregates upon mixing host and guest in vitro complicates capsid loading at low ionic strength, but can be sidestepped by increasing salt concentrations or diluting the components. Encapsulation of guests is completely reversible, and the position of the equilibrium is easily tuned by varying the ionic strength. These results, which challenge the notion that AaLS-13 is a continuous rigid shell, provide valuable information about cargo loading that will guide ongoing efforts to engineer functional host-guest complexes. Moreover, it should be possible to adapt the protein FRET pair described in this report to characterize functional capsid-cargo complexes generated by other encapsulation systems. PMID:26637019

  16. Secreted primary human malignant mesothelioma exosome signature reflects oncogenic cargo.

    Science.gov (United States)

    Greening, David W; Ji, Hong; Chen, Maoshan; Robinson, Bruce W S; Dick, Ian M; Creaney, Jenette; Simpson, Richard J

    2016-01-01

    Malignant mesothelioma (MM) is a highly-aggressive heterogeneous malignancy, typically diagnosed at advanced stage. An important area of mesothelioma biology and progression is understanding intercellular communication and the contribution of the secretome. Exosomes are secreted extracellular vesicles shown to shuttle cellular cargo and direct intercellular communication in the tumour microenvironment, facilitate immunoregulation and metastasis. In this study, quantitative proteomics was used to investigate MM-derived exosomes from distinct human models and identify select cargo protein networks associated with angiogenesis, metastasis, and immunoregulation. Utilising bioinformatics pathway/network analyses, and correlation with previous studies on tumour exosomes, we defined a select mesothelioma exosomal signature (mEXOS, 570 proteins) enriched in tumour antigens and various cancer-specific signalling (HPGD/ENO1/OSMR) and secreted modulators (FN1/ITLN1/MAMDC2/PDGFD/GBP1). Notably, such circulating cargo offers unique insights into mesothelioma progression and tumour microenvironment reprogramming. Functionally, we demonstrate that oncogenic exosomes facilitate the migratory capacity of fibroblast/endothelial cells, supporting the systematic model of MM progression associated with vascular remodelling and angiogenesis. We provide biophysical and proteomic characterisation of exosomes, define a unique oncogenic signature (mEXOS), and demonstrate the regulatory capacity of exosomes in cell migration/tube formation assays. These findings contribute to understanding tumour-stromal crosstalk in the context of MM, and potential new diagnostic and therapeutic extracellular targets. PMID:27605433

  17. Secreted primary human malignant mesothelioma exosome signature reflects oncogenic cargo

    Science.gov (United States)

    Greening, David W.; Ji, Hong; Chen, Maoshan; Robinson, Bruce W. S.; Dick, Ian M.; Creaney, Jenette; Simpson, Richard J.

    2016-01-01

    Malignant mesothelioma (MM) is a highly-aggressive heterogeneous malignancy, typically diagnosed at advanced stage. An important area of mesothelioma biology and progression is understanding intercellular communication and the contribution of the secretome. Exosomes are secreted extracellular vesicles shown to shuttle cellular cargo and direct intercellular communication in the tumour microenvironment, facilitate immunoregulation and metastasis. In this study, quantitative proteomics was used to investigate MM-derived exosomes from distinct human models and identify select cargo protein networks associated with angiogenesis, metastasis, and immunoregulation. Utilising bioinformatics pathway/network analyses, and correlation with previous studies on tumour exosomes, we defined a select mesothelioma exosomal signature (mEXOS, 570 proteins) enriched in tumour antigens and various cancer-specific signalling (HPGD/ENO1/OSMR) and secreted modulators (FN1/ITLN1/MAMDC2/PDGFD/GBP1). Notably, such circulating cargo offers unique insights into mesothelioma progression and tumour microenvironment reprogramming. Functionally, we demonstrate that oncogenic exosomes facilitate the migratory capacity of fibroblast/endothelial cells, supporting the systematic model of MM progression associated with vascular remodelling and angiogenesis. We provide biophysical and proteomic characterisation of exosomes, define a unique oncogenic signature (mEXOS), and demonstrate the regulatory capacity of exosomes in cell migration/tube formation assays. These findings contribute to understanding tumour-stromal crosstalk in the context of MM, and potential new diagnostic and therapeutic extracellular targets. PMID:27605433

  18. A Novel Multilayered RFID Tagged Cargo Integrity Assurance Scheme

    Directory of Open Access Journals (Sweden)

    Ming Hour Yang

    2015-10-01

    Full Text Available To minimize cargo theft during transport, mobile radio frequency identification (RFID grouping proof methods are generally employed to ensure the integrity of entire cargo loads. However, conventional grouping proofs cannot simultaneously generate grouping proofs for a specific group of RFID tags. The most serious problem of these methods is that nonexistent tags are included in the grouping proofs because of the considerable amount of time it takes to scan a high number of tags. Thus, applying grouping proof methods in the current logistics industry is difficult. To solve this problem, this paper proposes a method for generating multilayered offline grouping proofs. The proposed method provides tag anonymity; moreover, resolving disputes between recipients and transporters over the integrity of cargo deliveries can be expedited by generating grouping proofs and automatically authenticating the consistency between the receipt proof and pick proof. The proposed method can also protect against replay attacks, multi-session attacks, and concurrency attacks. Finally, experimental results verify that, compared with other methods for generating grouping proofs, the proposed method can efficiently generate offline grouping proofs involving several parties in a supply chain using mobile RFID.

  19. How molecular motors are arranged on a cargo is important for vesicular transport.

    Directory of Open Access Journals (Sweden)

    Robert P Erickson

    2011-05-01

    Full Text Available The spatial organization of the cell depends upon intracellular trafficking of cargos hauled along microtubules and actin filaments by the molecular motor proteins kinesin, dynein, and myosin. Although much is known about how single motors function, there is significant evidence that cargos in vivo are carried by multiple motors. While some aspects of multiple motor function have received attention, how the cargo itself--and motor organization on the cargo--affects transport has not been considered. To address this, we have developed a three-dimensional Monte Carlo simulation of motors transporting a spherical cargo, subject to thermal fluctuations that produce both rotational and translational diffusion. We found that these fluctuations could exert a load on the motor(s, significantly decreasing the mean travel distance and velocity of large cargos, especially at large viscosities. In addition, the presence of the cargo could dramatically help the motor to bind productively to the microtubule: the relatively slow translational and rotational diffusion of moderately sized cargos gave the motors ample opportunity to bind to a microtubule before the motor/cargo ensemble diffuses out of range of that microtubule. For rapidly diffusing cargos, the probability of their binding to a microtubule was high if there were nearby microtubules that they could easily reach by translational diffusion. Our simulations found that one reason why motors may be approximately 100 nm long is to improve their 'on' rates when attached to comparably sized cargos. Finally, our results suggested that to efficiently regulate the number of active motors, motors should be clustered together rather than spread randomly over the surface of the cargo. While our simulation uses the specific parameters for kinesin, these effects result from generic properties of the motors, cargos, and filaments, so they should apply to other motors as well.

  20. Final Report, Next-Generation Mega-Voltage Cargo-Imaging System for Cargo Conainer Inspection, March 2007

    Energy Technology Data Exchange (ETDEWEB)

    Dr. James Clayton, Ph.D., Varian Medical Systems-Security & Inspection Products; Dr. Emma Regentova, Ph.D, University of Nevada Las Vegas; Dr. Evangelos Yfantis, Ph.D., University of Nevada, Las Vegas

    2007-03-27

    The UNLV Research Foundation, as the primary award recipient, teamed with Varian Medical Systems-Security & Inspection Products and the University of Nevada Las Vegas (UNLV) for the purpose of conducting research and engineering related to a "next-generation" mega-voltage imaging (MVCI) system for inspection of cargo in large containers. The procurement and build-out of hardware for the MVCI project has been completed. The K-9 linear accelerator and an optimized X-ray detection system capable of efficiently detecting X-rays emitted from the accelerator after they have passed through the device is under test. The Office of Science financial assistance award has made possible the development of a system utilizing a technology which will have a profound positive impact on the security of U.S. seaports. The proposed project will ultimately result in critical research and development advances for the "next-generation" Linatron X-ray accelerator technology, thereby providing a safe, reliable and efficient fixed and mobile cargo inspection system, which will very significantly increase the fraction of cargo containers undergoing reliable inspection as the enter U.S. ports. Both NNSA/NA-22 and the Department of Homeland Security's Domestic Nuclear Detection Office are collaborating with UNLV and its team to make this technology available as soon as possible.

  1. Final Report, Next-Generation Mega-Voltage Cargo-Imaging System for Cargo Container Inspection, March 2007

    International Nuclear Information System (INIS)

    The UNLV Research Foundation, as the primary award recipient, teamed with Varian Medical Systems-Security and Inspection Products and the University of Nevada Las Vegas (UNLV) for the purpose of conducting research and engineering related to a ''next-generation'' mega-voltage imaging (MVCI) system for inspection of cargo in large containers. The procurement and build-out of hardware for the MVCI project has been completed. The K-9 linear accelerator and an optimized X-ray detection system capable of efficiently detecting X-rays emitted from the accelerator after they have passed through the device is under test. The Office of Science financial assistance award has made possible the development of a system utilizing a technology which will have a profound positive impact on the security of U.S. seaports. The proposed project will ultimately result in critical research and development advances for the ''next-generation'' Linatron X-ray accelerator technology, thereby providing a safe, reliable and efficient fixed and mobile cargo inspection system, which will very significantly increase the fraction of cargo containers undergoing reliable inspection as the enter U.S. ports. Both NNSA/NA-22 and the Department of Homeland Security's Domestic Nuclear Detection Office are collaborating with UNLV and its team to make this technology available as soon as possible

  2. Mechanics

    CERN Document Server

    Hartog, J P Den

    1961-01-01

    First published over 40 years ago, this work has achieved the status of a classic among introductory texts on mechanics. Den Hartog is known for his lively, discursive and often witty presentations of all the fundamental material of both statics and dynamics (and considerable more advanced material) in new, original ways that provide students with insights into mechanical relationships that other books do not always succeed in conveying. On the other hand, the work is so replete with engineering applications and actual design problems that it is as valuable as a reference to the practicing e

  3. A platform for actively loading cargo RNA to elucidate limiting steps in EV-mediated delivery

    OpenAIRE

    Leonard, Joshua N.; Hung, Michelle E.

    2016-01-01

    Extracellular vesicles (EVs) mediate intercellular communication through transfer of RNA and protein between cells. Thus, understanding how cargo molecules are loaded and delivered by EVs is of central importance for elucidating the biological roles of EVs and developing EV-based therapeutics. While some motifs modulating the loading of biomolecular cargo into EVs have been elucidated, the general rules governing cargo loading and delivery remain poorly understood. To investigate how general ...

  4. Analytical basis of technology asymmetrical allocation of cargo masses common centre in wagons

    Directory of Open Access Journals (Sweden)

    Khabibulla TURANOV

    2009-01-01

    Full Text Available The article gives the account of results of the analytical basis of technology asymmetrical cargo allocation in a wagon during rolling stock movement on track unevenness waves. The finite analytical formulas have been obtained for determination of response of wagon bogie spring sets in case of simultaneous displacement of cargo masses common centre longitudinally and sideways to the wagon, which make it possible to ground the cargo allocation according to admissible value of wagon vertical dynamic addition coefficient.

  5. 46 CFR 36.10-1 - Cargo pump relief valves-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo pump relief valves-TB/ALL. 36.10-1 Section 36.10-1..., Valves, Fittings, and Accessory Equipment § 36.10-1 Cargo pump relief valves—TB/ALL. (a) Cargo pump relief valves and pressure gages may be omitted, however, a suitable device shall be fitted to stop...

  6. A Framework for Analysis of Security Measures Within On-airport Cargo Facilities

    OpenAIRE

    Rountree, Carla; Demetsky, Michael J.

    2005-01-01

    The terrorist attacks of September 11, 2001 displayed the shortcomings of aviation security in the United States. Most of the attention on aviation security since that time has focused on airline passengers, their luggage, and their carry-on items, leaving air cargo security on the back burner. The lack of security screening and screening guidelines of cargo traveling by both passenger and all-cargo aircraft is the driving purpose behind this research project: the development of a framework t...

  7. Report on the 2011 and 2012 NASA Ames Research Center (ARC) / Alaska State Cargo Airship Workshops

    Science.gov (United States)

    Hochstettler, Ronald

    2012-01-01

    This presentation will summarize the Cargo Airships for Northern Operations workshop that was held August 24-25, 2011. This workshop co-sponsored by NASA ARC and the Alaska State Department of Transportation was initiated by interest from Alaska Lt. Governor Mead Treadwell for assistance in investigating the potential benefits of proposed cargo airships for the Alaskan economy and societal needs. The workshop provided a brief background on the technology and operational aspects of conventional airships and hybrids followed by presentations on issues affecting cargo airship operations such as weather management, insurance, regulations, crew duty/rest rules, and available support infrastructures. Speakers representing potential cargo airship users from Alaskan State and commercial organizations presented the needs they felt could be met by cargo airship services. Presenters from Canadian private and military interests also detailed applications and missions that cargo airships could provide to remote regions of Canada. Cost drivers of cargo airship operations were also addressed and tools for modeling and analyzing operational factors and costs affecting cargo airship operations were discussed. Four breakout sessions were held which allowed workshop participants to contribute inputs to four topic areas: Business Approaches and Strategies (financing incentives public/private partnerships etc) for Airship Development and Operation, Design, Development, Production Challenges, and Possible Solutions, Regulatory, Certification, Legal, and Insurance Issues, and Operational Issues, Customer Requirements, and Airship Requirements. A follow on to the 2011 cargo airship workshop is being planned for July 31 August 2, 2012. A status update on this second workshop will also be presented.

  8. X-ray scan detection for cargo integrity

    Science.gov (United States)

    Valencia, Juan; Miller, Steve

    2011-04-01

    The increase of terrorism and its global impact has made the determination of the contents of cargo containers a necessity. Existing technology allows non-intrusive inspections to determine the contents of a container rapidly and accurately. However, some cargo shipments are exempt from such inspections. Hence, there is a need for a technology that enables rapid and accurate means of detecting whether such containers were non-intrusively inspected. Non-intrusive inspections are most commonly performed utilizing high powered X-ray equipment. The challenge is creating a device that can detect short duration X-ray scans while maintaining a portable, battery powered, low cost, and easy to use platform. The Pacific Northwest National Laboratory (PNNL) has developed a methodology and prototype device focused on this challenge. The prototype, developed by PNNL, is a battery powered electronic device that continuously measures its X-ray and Gamma exposure, calculates the dose equivalent rate, and makes a determination of whether the device has been exposed to the amount of radiation experienced during an X-ray inspection. Once an inspection is detected, the device will record a timestamp of the event and relay the information to authorized personnel via a visual alert, USB connection, and/or wireless communication. The results of this research demonstrate that PNNL's prototype device can be effective at determining whether a container was scanned by X-ray equipment typically used for cargo container inspections. This paper focuses on laboratory measurements and test results acquired with the PNNL prototype device using several X-ray radiation levels.

  9. Photonuclear-based Detection of Nuclear Smuggling in Cargo Containers

    International Nuclear Information System (INIS)

    The Idaho National Engineering and Environmental Laboratory (INEEL) and the Los Alamos National Laboratory (LANL) have performed experiments in La Honda, California and at the Idaho Accelerator Center in Pocatello, Idaho to assess and develop a photonuclear-based detection system for shielded nuclear materials in cargo containers. The detection system, measuring photonuclear-related neutron emissions, is planned for integration with the ARACOR Eagle Cargo Container Inspection System (Sunnyvale, CA). The Eagle Inspection system uses a nominal 6-MeV electron accelerator and operates with safe radiation exposure limits to both container stowaways and to its operators. The INEEL has fabricated custom-built, helium-3-based, neutron detectors for this inspection application and is performing an experimental application assessment. Because the Eagle Inspection system could not be moved to LANL where special nuclear material was available, the response of the Eagle had to be determined indirectly so as to support the development and testing of the detection system. Experiments in California have successfully matched the delayed neutron emission performance of the ARACOR Eagle with that of the transportable INEEL electron accelerator (i.e., the Varitron) and are reported here. A demonstration test is planned at LANL using the Varitron and shielded special nuclear materials within a cargo container. Detector results are providing very useful information regarding the challenges of delayed neutron counting near the photofission threshold energy of 5.5 - 6.0 MeV, are identifying the possible utilization of prompt neutron emissions to allow enhanced signal-to-noise measurements, and are showing the overall benefits of using higher electron beam energies

  10. Photonuclear-based Detection of Nuclear Smuggling in Cargo Containers

    Science.gov (United States)

    Jones, J. L.; Haskell, K. J.; Hoggan, J. M.; Norman, D. R.; Yoon, W. Y.

    2003-08-01

    The Idaho National Engineering and Environmental Laboratory (INEEL) and the Los Alamos National Laboratory (LANL) have performed experiments in La Honda, California and at the Idaho Accelerator Center in Pocatello, Idaho to assess and develop a photonuclear-based detection system for shielded nuclear materials in cargo containers. The detection system, measuring photonuclear-related neutron emissions, is planned for integration with the ARACOR Eagle Cargo Container Inspection System (Sunnyvale, CA). The Eagle Inspection system uses a nominal 6-MeV electron accelerator and operates with safe radiation exposure limits to both container stowaways and to its operators. The INEEL has fabricated custom-built, helium-3-based, neutron detectors for this inspection application and is performing an experimental application assessment. Because the Eagle Inspection system could not be moved to LANL where special nuclear material was available, the response of the Eagle had to be determined indirectly so as to support the development and testing of the detection system. Experiments in California have successfully matched the delayed neutron emission performance of the ARACOR Eagle with that of the transportable INEEL electron accelerator (i.e., the Varitron) and are reported here. A demonstration test is planned at LANL using the Varitron and shielded special nuclear materials within a cargo container. Detector results are providing very useful information regarding the challenges of delayed neutron counting near the photofission threshold energy of 5.5 - 6.0 MeV, are identifying the possible utilization of prompt neutron emissions to allow enhanced signal-to-noise measurements, and are showing the overall benefits of using higher electron beam energies.

  11. Automatic continuous monitoring system for dangerous sites and cargoes

    International Nuclear Information System (INIS)

    The problems of creation of automatic comprehensive continuous monitoring system for nuclear and radiation sites and cargoes of Rosatom Corporation, which carries out data collecting, processing, storage and transmission, including informational support to decision-making, as well as support to modelling and forecasting functions, are considered. The system includes components of two levels: site and industry. Currently the system is used to monitor over 8000 integrated parameters, which characterise the status of nuclear and radiation safety on Rosatom sites, environmental and fire safety

  12. Scanner for the detection of contraband in air cargo containers

    International Nuclear Information System (INIS)

    Full text: There is a growing worldwide need to rapidly scan bulk air cargo for contraband such as illicit drugs and explosives. CSIRO has been working with Australian Customs Service to develop an innovative and cost-effective solution capable of directly scanning air freight containers in 1-2 minutes without unpacking. A new scanner has been developed that combines fast neutron and gamma-ray radiography to provide high-resolution images that include information on material composition. A full-scale prototype scanner has been successfully tested in the laboratory and a commercial-scale scanner will be installed at Brisbane airport in 2005. Copyright (2005) Australian Institute of Physics

  13. Advanced gamma ray technology for scanning cargo containers.

    Science.gov (United States)

    Orphan, Victor J; Muenchau, Ernie; Gormley, Jerry; Richardson, Rex

    2005-01-01

    The shipping industry is striving to increase security for cargo containers without significantly impeding traffic. Three Science Applications International Corporation (SAIC) development programs are supporting this effort. SAIC's ICIS system combines SAIC's VACIS gamma ray imaging, radiation scanning, OCR, elemental analysis and other technologies to scan containers for nuclear materials and other hazards in normal terminal traffic. SAIC's enhanced gamma ray detector improves VACIS image resolution by a factor of three. And SAIC's EmptyView software analyzes VACIS images to automatically verify empty containers. PMID:15996470

  14. High energy gamma ray telescope using a cargo airplane

    International Nuclear Information System (INIS)

    A new method to measure high energy gamma rays from astronomical objects is presented. In this method, we use commercial cargo airplanes to get the altitude near the air shower maximum. Gamma rays with the energy greater than 100 GeV can be detected by the lead-glass based detector. Directions of gamma rays are determined by those of the secondary electrons (positrons) in the showers. The angular resolution obtained by this method is considered to be better than 1 degree. The detector is sensitive to the gamma ray point sources. (author)

  15. Collinear swimmer propelling a cargo sphere at low Reynolds number

    CERN Document Server

    Felderhof, B U

    2014-01-01

    The swimming velocity and rate of dissipation of a linear chain consisting of two or three little spheres and a big sphere is studied on the basis of low Reynolds number hydrodynamics. The big sphere is treated as a passive cargo, driven by the tail of little spheres via hydrodynamic and direct elastic interaction. The fundamental solution of Stokes' equations in the presence of a sphere with no-slip boundary condition, as derived by Oseen, is used to model the hydrodynamic interactions between the big sphere and the little spheres.

  16. Advanced γ ray technology for scanning cargo containers

    International Nuclear Information System (INIS)

    The shipping industry is striving to increase security for cargo containers without significantly impeding traffic. Three Science Applications International Corporation (SAIC) development programs are supporting this effort. SAIC's ICIS system combines SAIC's VACIS[reg] γ ray imaging, radiation scanning, OCR, elemental analysis and other technologies to scan containers for nuclear materials and other hazards in normal terminal traffic. SAIC's enhanced γ ray detector improves VACIS image resolution by a factor of three. And SAIC's EmptyView software analyzes VACIS images to automatically verify empty containers

  17. Optimization of the imported air express cargo distribution problem

    Directory of Open Access Journals (Sweden)

    Hwang, T.L.

    2013-03-01

    Full Text Available This study examines the delivering network of imported air express cargo as an integrated multi-depot vehicle routing problem. Integrated multi-depot vehicle routing problem attempts to decide which service centers should be used and how much freight should be unloaded in each service center. The role of an exchange point which is allowing the delivery vans and shuttles to exchange imported and exported goods is also addressed. Test results demonstrate the feasibility of the four models so these are highly promising for use in a diverse array of applications, such as in home delivery and reverse logistics.

  18. The legal and documentary aspects of sale of oil cargoes

    International Nuclear Information System (INIS)

    The paper is divided into three main sections. The first part discusses a number of specific areas where sale contracts in the oil markets differ from traditional CIF or FOB contracts in other trades and where difficulties have therefore arisen which are peculiar to the oil trade. The second part deals with the passing of ownership and risk in oil cargoes. Finally it looks at certain documentary aspects of oil trading transactions, again concentrating on areas such as letters of indemnity, where the oil markets differ from the traditional commodity markets. (author)

  19. Achieving integrated convoys: cargo unmanned ground vehicle development and experimentation

    Science.gov (United States)

    Zych, Noah; Silver, David; Stager, David; Green, Colin; Pilarski, Thomas; Fischer, Jacob

    2013-05-01

    The Cargo UGV project was initiated in 2010 with the aim of developing and experimenting with advanced autonomous vehicles capable of being integrated unobtrusively into manned logistics convoys. The intent was to validate two hypotheses in complex, operationally representative environments: first, that unmanned tactical wheeled vehicles provide a force protection advantage by creating standoff distance to warfighters during ambushes or improvised explosive device attacks; and second, that these UGVs serve as force multipliers by enabling a single operator to control multiple unmanned assets. To assess whether current state-of-the-art autonomous vehicle technology was sufficiently capable to permit resupply missions to be executed with decreased risk and reduced manpower, and to assess the effect of UGVs on customary convoy tactics, the Marine Corps Warfighting Laboratory and the Joint Ground Robotics Enterprise sponsored Oshkosh Defense and the National Robotics Engineering Center to equip two standard Marine Corps cargo trucks for autonomous operation. This paper details the system architecture, hardware implementation, and software modules developed to meet the vehicle control, perception, and planner requirements compelled by this application. Additionally, the design of a custom human machine interface and an accompanying training program are described, as is the creation of a realistic convoy simulation environment for rapid system development. Finally, results are conveyed from a warfighter experiment in which the effectiveness of the training program for novice operators was assessed, and the impact of the UGVs on convoy operations was observed in a variety of scenarios via direct comparison to a fully manned convoy.

  20. Vehicle and cargo container inspection system for drugs

    Science.gov (United States)

    Verbinski, Victor V.; Orphan, Victor J.

    1999-06-01

    A vehicle and cargo container inspection system has been developed which uses gamma-ray radiography to produce digital images useful for detection of drugs and other contraband. The system is comprised of a 1 Ci Cs137 gamma-ray source collimated into a fan beam which is aligned with a linear array of NaI gamma-ray detectors located on the opposite side of the container. The NaI detectors are operated in the pulse-counting mode. A digital image of the vehicle or container is obtained by moving the aligned source and detector array relative to the object. Systems have been demonstrated in which the object is stationary (source and detector array move on parallel tracks) and in which the object moves past a stationary source and detector array. Scanning speeds of ˜30 cm/s with a pixel size (at the object) of ˜1 cm have been achieved. Faster scanning speeds of ˜2 m/s have been demonstrated on railcars with more modest spatial resolution (4 cm pixels). Digital radiographic images are generated from the detector count rates. These images, recorded on a PC-based data acquisition and display system, are shown from several applications: 1) inspection of trucks and containers at a border crossing, 2) inspection of railcars at a border crossing, 3) inspection of outbound cargo containers for stolen automobiles, and 4) inspection of trucks and cars for terrorist bombs.

  1. Vehicle and cargo container inspection system for drugs

    International Nuclear Information System (INIS)

    A vehicle and cargo container inspection system has been developed which uses gamma-ray radiography to produce digital images useful for detection of drugs and other contraband. The system is comprised of a 1 Ci Cs137 gamma-ray source collimated into a fan beam which is aligned with a linear array of NaI gamma-ray detectors located on the opposite side of the container. The NaI detectors are operated in the pulse-counting mode. A digital image of the vehicle or container is obtained by moving the aligned source and detector array relative to the object. Systems have been demonstrated in which the object is stationary (source and detector array move on parallel tracks) and in which the object moves past a stationary source and detector array. Scanning speeds of ∼30 cm/s with a pixel size (at the object) of ∼1 cm have been achieved. Faster scanning speeds of ∼2 m/s have been demonstrated on railcars with more modest spatial resolution (4 cm pixels). Digital radiographic images are generated from the detector count rates. These images, recorded on a PC-based data acquisition and display system, are shown from several applications: 1) inspection of trucks and containers at a border crossing, 2) inspection of railcars at a border crossing, 3) inspection of outbound cargo containers for stolen automobiles, and 4) inspection of trucks and cars for terrorist bombs

  2. Cargo inspection system based on pulsed fast neutron analysis

    Science.gov (United States)

    Brown, Douglas R.; Coates, Allison; Kuo, Stelly N.; Loveman, Robert; Pentaleri, Ed; Rynes, Joel C.

    1997-02-01

    The pulsed fast neutron analysis (PFNA) cargo inspection system (CIS) uses a nanosecond pulsed beam of fast neutrons to interrogate the contents of small volume elements -- voxels -- of a cargo container or truck. A color display shows the three-dimensional location of suspected contraband, such as drugs or explosives. The neutrons interact with the elemental contents of each vowel, and gamma rays characteristic of the elements are collected in an array of detectors. The elemental signals and their ratios give unique signatures for drugs and other contraband. From the time of arrival of the gamma rays, the position of the vowel within the truck is determined. The PFNA CIS is designed to scan five or more trucks per hour. The operator interface has been designed to assist in the rapid identification of drugs, explosives or other contraband. This paper describes the system and the tests for drugs and explosives that have been carried out during the past year. These tests were aimed at exploring the envelope of performance of the system.

  3. The salvaging of the Mont Louis cargo ship

    International Nuclear Information System (INIS)

    On Saturday the 25th of August 1984, at 2:20 P.M., the German car ferry Olau Britannia with 800 passengers aboard rammed the rear starboard side of the French cargo ship Mont Louis. The collision occurred in the English Channel at a point 18 kilometers north of Ostend. At 7:00 P.M. the Mont Louis sank to a depth of 15 meters at a distance of several miles by rail from Antwerp. Half the ship's hull lay uncovered at low tide. The cargo included 30 type 48-Y containers, each filled with 12 tons of UF6, and 22 empty type 30-B containers for return of the enriched uranium. The UF6 was to be enriched to 4% in the Soviet Union for use as fuel in nuclear power plants. The table below details the nature of the UF6. After salvaging operation the containers have been inspected and no damage or leaks have been detected. The accident involved no risk of radioactive or chemical contamination of the sea water. The systematic misinformation campaign by Greenpeace and news media is briefly described. (orig./HP)

  4. Relocatable cargo x-ray inspection systems utilizing compact linacs

    International Nuclear Information System (INIS)

    Magnetron-powered, X-band linacs with 3-4 MeV capability are compact enough to be readily utilized in relocatable high energy cargo inspection systems. Just such a system is currently under development at AS and E trade mark sign using the commercially available ISOSearch trade mark sign cargo inspection system as the base platform. The architecture permits the retention of backscatter imaging, which has proven to be an extremely valuable complement to the more usual transmission images. The linac and its associated segmented detector will provide an additional view with superior penetration and spatial resolution. The complete system, which is housed in two standard 40' ISO containers, is briefly described with emphasis on the installation and operating characteristics of the portable linac. The average rf power delivered by the magnetron to the accelerator section can be varied up to the maximum of about 1 kW. The projected system performance, including radiation dose to the environment, will be discussed and compared with other high energy systems

  5. 49 CFR 178.320 - General requirements applicable to all DOT specification cargo tank motor vehicles.

    Science.gov (United States)

    2010-10-01

    .... Lading means the hazardous material contained in a cargo tank. Loading/unloading connection means the... cargo tank loading/unloading outlet to which the loading/unloading connection is attached. Manufacturer... means for attaching a closure), except that the following are not outlets: a threaded opening...

  6. 46 CFR 105.25-5 - Compartments or areas containing cargo tanks or pumping systems.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Compartments or areas containing cargo tanks or pumping systems. 105.25-5 Section 105.25-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO... or pumping systems. (a) Compartments or areas containing tanks or pumping systems shall be closed...

  7. 46 CFR 308.551 - War risk insurance clearing agency agreement for cargo, Form MA-321.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false War risk insurance clearing agency agreement for cargo... EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Iv-General § 308.551 War risk insurance... domestic insurance companies authorized to do a marine insurance business in any State of the United...

  8. 75 FR 9638 - Safety Advisory Notice: Use of Composite Cargo Tanks Manufactured Under DOT Special Permits

    Science.gov (United States)

    2010-03-03

    ... reinforced plastic (GFRP) cargo tanks. Federal hazardous materials transportation law (Federal hazmat law; 49..., and use of FRP or GFRP cargo tank motor vehicles: Expiration SP number Holder Status date 9166... above contain the following requirement: The compatibility of the commodities and the (FRP or...

  9. 46 CFR 153.981 - Leaving room in tank for cargo expansion.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Leaving room in tank for cargo expansion. 153.981 Section 153.981 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS... Transfer Procedures § 153.981 Leaving room in tank for cargo expansion. The person in charge of...

  10. 75 FR 1070 - Cargo Securing Methods for Packages in Transport Vehicles or Freight Containers

    Science.gov (United States)

    2010-01-08

    ... notice regarding our public dockets in the January 17, 2008, issue of the Federal Register (73 FR 3316... SECURITY Coast Guard Cargo Securing Methods for Packages in Transport Vehicles or Freight Containers AGENCY... the public on methods for securing cargo in transport vehicles and freight containers in order...

  11. 46 CFR 31.10-16 - Inspection and certification of cargo gear-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Inspection and certification of cargo gear-TB/ALL. 31.10... CERTIFICATION Inspections § 31.10-16 Inspection and certification of cargo gear—TB/ALL. (a) The owner, operator... treatment of chains, rings, hooks, shackles, and swivels which require such treatment; and,...

  12. 33 CFR 126.29 - Supervision and control of dangerous cargo.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Supervision and control of... Supervision and control of dangerous cargo. (a) Authority. The Captain of the Port is authorized to require... cargo covered by this subchapter shall be undertaken and continued only under the immediate...

  13. 46 CFR 32.60-15 - Segregation of cargo; Grade E-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Segregation of cargo; Grade E-TB/ALL. 32.60-15 Section 32.60-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL EQUIPMENT... § 32.60-15 Segregation of cargo; Grade E—TB/ALL. (a) General. The galleys, living quarters,...

  14. 19 CFR 123.91 - Electronic information for rail cargo required in advance of arrival.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Electronic information for rail cargo required in advance of arrival. 123.91 Section 123.91 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY CUSTOMS RELATIONS WITH CANADA AND MEXICO Advance Information for Cargo Arriving by Rail or...

  15. 19 CFR 123.92 - Electronic information for truck cargo required in advance of arrival.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Electronic information for truck cargo required in advance of arrival. 123.92 Section 123.92 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY CUSTOMS RELATIONS WITH CANADA AND MEXICO Advance Information for Cargo Arriving by Rail or...

  16. 14 CFR 121.198 - Cargo service airplanes: Increased zero fuel and landing weights.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Cargo service airplanes: Increased zero... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Airplane Performance Operating Limitations § 121.198 Cargo service airplanes: Increased zero fuel and landing...

  17. 76 FR 4709 - Extension of Agency Information Collection Activity Under OMB Review: Certified Cargo Screening...

    Science.gov (United States)

    2011-01-26

    ... off- airport and includes facilities upstream in the air cargo supply chain, such as shippers... supply chain to accept, screen, and transport air cargo. The uninterrupted collection of this information... below to the Office of Management and Budget (OMB) for renewal in compliance with the...

  18. 49 CFR 1549.111 - Security threat assessments for personnel of certified cargo screening facilities.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Security threat assessments for personnel of... SECURITY CERTIFIED CARGO SCREENING PROGRAM Operations § 1549.111 Security threat assessments for personnel... cargo screening facility, an indirect air carrier under 49 CFR part 1548 for transport on a...

  19. 46 CFR 308.501 - Cargoes on which coverage is available.

    Science.gov (United States)

    2010-10-01

    ... INSURANCE War Risk Cargo Insurance I-Introduction § 308.501 Cargoes on which coverage is available. The Maritime Administrator will be prepared to provide marine insurance against loss or damage by the risks of... Merchant Marine Act, 1936, as amended (46 App. U.S.C. 1281(a)); (b) Shipped or to be shipped on any...

  20. 46 CFR 31.40-5 - Cargo Ship Safety Construction Certificate-T/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate-T/ALL. 31.40... Safety Construction Certificate—T/ALL. (a) All tankships on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by the U.S....

  1. 49 CFR 173.33 - Hazardous materials in cargo tank motor vehicles.

    Science.gov (United States)

    2010-10-01

    ... pressure in accordance with 49 CFR 180.405(k). (3) Any Specification MC 310 or MC 311 cargo tank motor... this subchapter. Editorial Note: For Federal Register citations affecting § 173.33, see the List of CFR... 49 Transportation 2 2010-10-01 2010-10-01 false Hazardous materials in cargo tank motor...

  2. Mechanics

    CERN Document Server

    Chester, W

    1979-01-01

    When I began to write this book, I originally had in mind the needs of university students in their first year. May aim was to keep the mathematics simple. No advanced techniques are used and there are no complicated applications. The emphasis is on an understanding of the basic ideas and problems which require expertise but do not contribute to this understanding are not discussed. How­ ever, the presentation is more sophisticated than might be considered appropri­ ate for someone with no previous knowledge of the subject so that, although it is developed from the beginning, some previous acquaintance with the elements of the subject would be an advantage. In addition, some familiarity with element­ ary calculus is assumed but not with the elementary theory of differential equations, although knowledge of the latter would again be an advantage. It is my opinion that mechanics is best introduced through the motion of a particle, with rigid body problems left until the subject is more fully developed. Howev...

  3. ATHLETE: A Cargo-Handling Vehicle for Solar System Exploration

    Science.gov (United States)

    Wilcox, Brian H.

    2011-01-01

    As part of the NASA Exploration Technology Development Program, the Jet Propulsion Laboratory is developing a vehicle called ATHLETE: the All-Terrain Hex-Limbed Extra-Terrestrial Explorer. Each vehicle is based on six wheels at the ends of six multi-degree-of-freedom limbs. Because each limb has enough degrees of freedom for use as a general-purpose leg, the wheels can be locked and used as feet to walk out of excessively soft or other extreme terrain. Since the vehicle has this alternative mode of traversing through or at least out of extreme terrain, the wheels and wheel actuators can be sized for nominal terrain. There are substantial mass savings in the wheel and wheel actuators associated with designing for nominal instead of extreme terrain. These mass savings are comparable-to or larger-than the extra mass associated with the articulated limbs. As a result, the entire mobility system, including wheels and limbs, can be about 25% lighter than a conventional mobility chassis. A side benefit of this approach is that each limb has sufficient degrees-of-freedom to use as a general-purpose manipulator (hence the name "limb" instead of "leg"). Our prototype ATHLETE vehicles have quick-disconnect tool adapters on the limbs that allow tools to be drawn out of a "tool belt" and maneuvered by the limb. A power-take-off from the wheel actuates the tools, so that they can take advantage of the 1+ horsepower motor in each wheel to enable drilling, gripping or other power-tool functions. Architectural studies have indicated that one useful role for ATHLETE in planetary (moon or Mars) exploration is to "walk" cargo off the payload deck of a lander and transport it across the surface. Recent architectural approaches are focused on the concept that the lander descent stage will use liquid hydrogen as a propellant. This is the highest performance chemical fuel, but it requires very large tanks. A natural geometry for the lander is to have a single throttleable rocket engine on

  4. Multirole cargo aircraft options and configurations. [economic analysis

    Science.gov (United States)

    Conner, D. W.; Vaughan, J. C., III

    1979-01-01

    A future requirements and advanced market evaluation study indicates derivatives of current wide-body aircraft, using 1980 advanced technology, would be economically attractive through 2008, but new dedicated airfreighters incorporating 1990 technology, would offer little or no economic incentive. They would be economically attractive for all payload sizes, however, if RD and T costs could be shared in a joint civil/military arrangement. For the 1994-2008 cargo market, option studies indicate Mach 0.7 propfans would be economically attractive in trip cost, aircraft price and airline ROI. Spanloaders would have an even lower price and higher ROI but would have a relatively high trip cost because of aerodynamic inefficiencies. Dedicated airfreighters using propfans at Mach 0.8 cruise, laminar flow control, or cryofuels, would not provide any great economic benefits. Air cushion landing gear configurations are identified as an option for avoiding runway constraints on airport requirements and/or operational constraints are noted.

  5. A Sensor and Communications System for Containerized-Cargo Security

    Energy Technology Data Exchange (ETDEWEB)

    Leach Jr., R R

    2005-02-10

    A public/private collaboration between federal, state, provincial, and local U.S. and Canadian governmental organizations, called the Canada - United States Cargo Security Project has been formed, with the goal to improve security of containerized cargo moving from overseas locations into eastern Canadian provinces and the Northeastern United States. The current phase of this project has two technical objectives. These are: (1) to build and test a prototype in-container sensor system able to detect unauthorized entry into the container and the presence of radioactive material, to record geographical location and environmental data, and to transmit this information via satellite communications to a remote monitoring facility, and (2) to develop a secure website where data from the in-container sensors and other information will be displayed in real or near-real time and can be made available to law enforcement and emergency response organizations as appropriate. This paper will describe these activities, currently being undertaken by the Lawrence Livermore National Laboratory. An additional goal of the project's current phase is to integrate multi-jurisdictional training and first-responder exercises while monitoring and tracking container shipments from overseas to the US via Canadian ports-of-entry into North America. This activity is being undertaken by other project partners, which include the National Infrastructure Institute--Center for Infrastructure Expertise (NI2CIE), Transport Canada, Canadian Provinces of Quebec and Nova Scotia, Ports of Halifax and Montreal, U.S. Coast Guard (First Coast Guard District), States of New Hampshire, Maine, Vermont, and New York and the Port of Portland.

  6. EL VÍNCULO INDIVIDUO-COLECTIVO EN EL SISTEMA DE CARGOS EN UNA COMUNIDAD RURAL DEL SUR DE TLAXCALA, MÉXICO

    Directory of Open Access Journals (Sweden)

    Hernán Salas Quintanal

    2013-12-01

    Full Text Available This paper exposes the authors reflections about their last investigations into  community organization concept. The research took place in several countries in Mexico from 2008 to 2012. It is an anthropological analysis about cargo system, which includes native and no-native communities. In such a way, the authors recognize the importance of heterogeneous institution into cargo system concept. So that, they suggest, as the main hypothesis, that cargo system creates several mechanisms, which promote that members of the community stimulated social interactions’ scenes into role set. First, the community institutions promote the participation of the largest number of people. Second, interactions’ scenes are constantly created and recreated by the community members. Third, the roles’ rotation, as a product of a tacit agreement into community members, is fundamental to assure social participation, scene by scene. In order to facilitate the analysis, such mechanisms have been categorised in four larger dimensions: political-judicial, economic, religious and social. As a final thought, the idea of a communitarian project becomes the common characteristic of all the studied communities.

  7. El sistema de cargos en San Bernardino de Milpillas Chico, Durango

    OpenAIRE

    Chantal Cramaussel

    2013-01-01

    La principal estructura política de los tepehuanos es el sistema de cargos. En San Bernardino de Milpillas, Durango, los cargos de la Iglesia son diferentes a los del gobierno tradicional. Los segundos parecen apegarse más a las culturales locales, aunque los cargos de la Iglesia han sido objeto de transformaciones significativas para excluir a los ministros católicos en las ceremonias cristianas. La fiesta de la Candelaria, que precede a la toma de posesión de los nuevos “empleados”, muestra...

  8. Local synaptic signaling enhances the stochastic transport of motor-driven cargo in neurons

    OpenAIRE

    Newby, J. M.; Bressloff, P. C.

    2010-01-01

    The tug-of-war model of motor-driven cargo transport is formulated as an intermittent trapping process. An immobile trap, representing the cellular machinery that sequesters a motor-driven cargo for eventual use, is located somewhere within a microtubule track. A particle representing a motor-driven cargo that moves randomly with a forward bias is introduced at the beginning of the track. The particle switches randomly between a fast moving phase and a slow moving phase. When in the slow movi...

  9. 46 CFR 154.1205 - Mechanical ventilation system: Standards.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Mechanical ventilation system: Standards. 154.1205... Equipment Cargo Area: Mechanical Ventilation System § 154.1205 Mechanical ventilation system: Standards. (a) Each exhaust type mechanical ventilation system required under § 154.1200 (a) must have ducts...

  10. Lemur tyrosine kinase-2 signalling regulates kinesin-1 light chain-2 phosphorylation and binding of Smad2 cargo.

    LENUS (Irish Health Repository)

    Manser, C

    2012-05-31

    A recent genome-wide association study identified the gene encoding lemur tyrosine kinase-2 (LMTK2) as a susceptibility gene for prostate cancer. The identified genetic alteration is within intron 9, but the mechanisms by which LMTK2 may impact upon prostate cancer are not clear because the functions of LMTK2 are poorly understood. Here, we show that LMTK2 regulates a known pathway that controls phosphorylation of kinesin-1 light chain-2 (KLC2) by glycogen synthase kinase-3β (GSK3β). KLC2 phosphorylation by GSK3β induces the release of cargo from KLC2. LMTK2 signals via protein phosphatase-1C (PP1C) to increase inhibitory phosphorylation of GSK3β on serine-9 that reduces KLC2 phosphorylation and promotes binding of the known KLC2 cargo Smad2. Smad2 signals to the nucleus in response to transforming growth factor-β (TGFβ) receptor stimulation and transport of Smad2 by kinesin-1 is required for this signalling. We show that small interfering RNA loss of LMTK2 not only reduces binding of Smad2 to KLC2, but also inhibits TGFβ-induced Smad2 signalling. Thus, LMTK2 may regulate the activity of kinesin-1 motor function and Smad2 signalling.

  11. 46 CFR 38.15-5 - Cargo hose-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... the cargo are carried on board the vessel, they shall be of flexible metal and fabricated of seamless steel pipe and flexible joints of steel or bronze, or of other suitable material resistant to the...

  12. A cargo inspection system based on pulsed fast neutron analysis (PFNATM)

    International Nuclear Information System (INIS)

    A cargo inspection system based on pulsed fast neutron analysis (PFNATM) is to be used at a border crossing to detect explosives and contraband hidden in trucks and cargo containers. Neutrons are produced by the interaction of deuterons in a deuterium target mounted on a moveable scan arm. The collimated pulsed fast neutron beam is used to determine the location and composition of objects in a cargo container. The neutrons produce secondary gamma rays that are characteristic of the object's elemental composition. The cargo inspection system building consists of an accelerator room and an inspection tunnel. The accelerator room is shielded and houses the injector, accelerator and the neutron production gas target. The inspection tunnel is partially shielded. The truck or container to be inspected will be moved through the inspection tunnel by a conveyor system. The facility and radiation source terms considered in the shielding design are described. (authors)

  13. Incorporating biplane wing theory into a large, subsonic, all-cargo transport

    Science.gov (United States)

    Zyskowski, Michael K.

    1994-01-01

    If the air-cargo market increases at the pace predicted, a new conceptual aircraft will be demanded to meet the needs of the air-cargo industry. Furthermore, it has been found that not only should this aircraft be optimized to carry the intermodal containers used by the current shipping industry, but it should also be be able to operate at existing airports. The best solution to these problems is a configuration incorporating a bi-wing planform, which has resulted in significant improvements over the monoplane in lift/drag, weight reduction, and span reduction. The future of the air-cargo market, biplane theory, wind tunnel tests, and a comparison of the aerodynamic characteristics of the biplane and monoplane are discussed. The factors pertaining to a biplane cargo transport are then examined, resulting in biplane geometric parameters.

  14. Recent developments in fast neutron radiography for the interrogation of air cargo containers

    International Nuclear Information System (INIS)

    There is a worldwide need for improved methods for the scanning of consolidated air cargo for contraband such as illicit drugs and explosives. Ideally, cargo containers must be imaged without unpacking and with scan times of less than a few minutes. Fast neutron radiography techniques are particularly attractive for screening cargo. Neutrons have the required penetration, they interact with matter in a manner complementary to X-rays and they can be used to determine cargo composition. The Commonwealth Science and Industrial Research Organisation (CSIRO) has developed a scanner for fully-loaded air cargo containers. The scanner combines fast (14 MeV) neutron and γ-ray (or X-ray) radiography, using intense radiation sources and custom high-efficiency detector arrays. The ratio of the transmissions of neutrons and X-rays provides a measure of material composition that is much more sensitive than alternative dual high-energy (MeV) X-ray systems. A full-scale prototype scanner was used by Australian Customs Service to screen incoming air cargo at Brisbane International Airport in 2005/6. The trial of the scanner at Brisbane demonstrated the material discrimination capability of the technology and its ability to make hidden organic materials more obvious. Consolidated cargo was scanned in less than two minutes allowing high volumes of cargo to be screened rapidly. CSIRO is working directly with Nuctech Company Limited, Beijing, China to develop and commercialise the next generation in air cargo scanning technology. A commercial version of the airport scanner being developed by Nuctech and CSIRO is expected to be commissioned by January 2009. The commercial scanner combines a 14 MeV fast neutron radiography system with Nuctech's dual-energy X-ray technology that uses a 6 MeV LINAC X-ray source and Binocular Stereoscopic imaging technology. The commercial scanner will have much better spatial resolution than the Brisbane scanner. The improved resolution, combined with

  15. An 18680 dwt Multipurpose/Heavy Lift Cargo Vessel, Part II

    OpenAIRE

    Grubišić, Rajko; Grubiša, Robert; Rimanić, Radojka; Vlah, Radivoj; Kučan, Edi; Štokić, Miroslav

    2009-01-01

    In the article the second part of the signifi cant design features of an 18680 dwt multipurpose/ heavy lift cargo vessel is presented. The specifi c features of the ship structure design are fi rst dealt with. Then, the propulsion assembly, including the main engine, the gearbox/coupling and the controllable pitch propeller system, is described. Also, features of the electrical system are given, with special emphasis laid on the supply, driving and controlling system of the heavy cargo dec...

  16. Hybridization of General Cargo Ships to meet the Required Energy Efficiency Design Index

    OpenAIRE

    Øverleir, Magnus Anders

    2015-01-01

    In this thesis a hybrid propulsion system is proposed for a general cargo ship with the aim to meet the required Energy Efficiency Design Index (EEDI). The study has investigated how a hybrid propulsion system will influence the ship s EEDI value and fuel economy. The central problem is the coming challenge for the general cargo segment meeting the required efficiency value. Especially small vessels (3 000-15 000 DWT) with high speed will have troubles complying with the stricter regulations....

  17. A study of international security requirement on Hong Kong air cargo industry : development and challenges

    OpenAIRE

    Tang, Yu-to, Matthew; 鄧汝滔

    2012-01-01

    Currently, most of the research related to aviation sector of Hong Kong would focus on infrastructure perspective such as the building of 3rd runway in HKIA and the cooperation issues among airports in Pearl River Delta (PRD) Region in order to analyze the impact towards competitiveness of Hong Kong as a major international air cargo hub. However, there are not much research on air cargo industry has been done from security perspective but the recent trend of tightening international security...

  18. Research on combination forecast of port cargo throughput based on time series and causality analysis

    Directory of Open Access Journals (Sweden)

    Chi Zhang

    2013-03-01

    Full Text Available Purpose: The purpose of this paper is to develop a combined model composed of grey-forecast model and Logistic-growth-curve model to improve the accuracy of forecast model of cargo throughput for the port. The authors also use the existing data of a current port to verify the validity of the combined model.Design/methodology/approach: A literature review is undertaken to find the appropriate forecast model of cargo throughput for the port. Through researching the related forecast model, the authors put together the individual models which are significant to study further. Finally, the authors combine two individual models (grey-forecast model and Logistic-growth-curve model into one combined model to forecast the port cargo throughput, and use the model to a physical port in China to testify the validity of the model.Findings: Test by the perceptional data of cargo throughput in the physical port, the results show that the combined model can obtain relatively higher forecast accuracy when it is not easy to find more information. Furthermore, the forecast made by the combined model are more accurate than any of the individual ones.Research limitations/implications: The study provided a new combined forecast model of cargo throughput with a relatively less information to improve the accuracy rate of the forecast. The limitation of the model is that it requires the cargo throughput of the port have an S-shaped change trend.Practical implications: This model is not limited by external conditions such as geographical, cultural. This model predicted the port cargo throughput of one real port in China in 2015, which provided some instructive guidance for the port development.Originality/value: This is the one of the study to improve the accuracy rate of the cargo throughput forecast with little information.

  19. Oligomerization of a Cargo Receptor Directs Protein Sorting into COPII-coated Transport Vesicles

    OpenAIRE

    Sato, Ken; Nakano, Akihiko

    2003-01-01

    Secretory proteins are transported from the endoplasmic reticulum (ER) to the Golgi complex in vesicles coated with coat protein complex II (COPII). The incorporation of certain transport molecules (cargo) into the COPII vesicles is thought to be mediated by cargo receptors. Here we show that Emp47p, a type-I membrane protein, is specifically required for the transport of an integral membrane protein, Emp46p, from the ER. Exit of Emp46p from the ER was saturable and de...

  20. Mulheres em cargos profissionais de chefia: o paradoxo da igualdade

    Directory of Open Access Journals (Sweden)

    Fabia Alberton de Silva Galvane

    2015-12-01

    Full Text Available Resumo O artigo aqui apresentado foi construído a partir de informações obtidas em uma pesquisa realizada no ano de 2009, apresentada como Trabalho de Conclusão de Curso da Psicologia, ao Centro Universitário Barriga Verde - UNIBAVE (GALVANE, 2009. A pesquisa teve como objetivo geral verificar os sentidos produzidos por mulheres que ocupam cargos de chefia em indústrias de uma cidade do sul de Santa Catarina, Brasil. O modelo de pesquisa foi o qualitativo proposto por González Rey (2005. Os sujeitos da pesquisa foram sete mulheres e as informações obtidas por meio de entrevistas semiestruturadas. Neste artigo, propõe-se revisar informações obtidas e ampliar discussões outrora realizadas, utilizando-se das contribuições teóricas de Joan Scott e Judith Butler. Entre outras questões relevantes, o estudo sugere que as entrevistadas utilizam concepções baseadas em “certa essência feminina” como estratégia de visibilidade, permanência e poder no mercado de trabalho.

  1. The complex network of global cargo ship movements

    CERN Document Server

    Kaluza, Pablo; Gastner, Michael T; Blasius, Bernd

    2010-01-01

    Transportation networks play a crucial role in human mobility, the exchange of goods, and the spread of invasive species. With 90% of world trade carried by sea, the global network of merchant ships provides one of the most important modes of transportation. Here we use information about the itineraries of 16,363 cargo ships during the year 2007 to construct a network of links between ports. We show that the network has several features which set it apart from other transportation networks. In particular, most ships can be classified in three categories: bulk dry carriers, container ships and oil tankers. These three categories do not only differ in the ships' physical characteristics, but also in their mobility patterns and networks. Container ships follow regularly repeating paths whereas bulk dry carriers and oil tankers move less predictably between ports. The network of all ship movements possesses a heavy-tailed distribution for the connectivity of ports and for the loads transported on the links with s...

  2. Desktop Application Program to Simulate Cargo-Air-Drop Tests

    Science.gov (United States)

    Cuthbert, Peter

    2009-01-01

    The DSS Application is a computer program comprising a Windows version of the UNIX-based Decelerator System Simulation (DSS) coupled with an Excel front end. The DSS is an executable code that simulates the dynamics of airdropped cargo from first motion in an aircraft through landing. The bare DSS is difficult to use; the front end makes it easy to use. All inputs to the DSS, control of execution of the DSS, and postprocessing and plotting of outputs are handled in the front end. The front end is graphics-intensive. The Excel software provides the graphical elements without need for additional programming. Categories of input parameters are divided into separate tabbed windows. Pop-up comments describe each parameter. An error-checking software component evaluates combinations of parameters and alerts the user if an error results. Case files can be created from inputs, making it possible to build cases from previous ones. Simulation output is plotted in 16 charts displayed on a separate worksheet, enabling plotting of multiple DSS cases with flight-test data. Variables assigned to each plot can be changed. Selected input parameters can be edited from the plot sheet for quick sensitivity studies.

  3. The Lethal Cargo of Myxococcus xanthus Outer Membrane Vesicles

    Directory of Open Access Journals (Sweden)

    JamesEBerleman

    2014-09-01

    Full Text Available Myxococcus xanthus is a bacterial micro-predator known for hunting other microbes in a wolf pack-like manner. Outer membrane vesicles (OMVs are produced in large quantities by M. xanthus and have a highly organized structure in the extracellular milieu, sometimes occurring in chains that link neighboring cells within a biofilm. OMVs may be a vehicle for mediating wolf pack activity by delivering hydrolytic enzymes and antibiotics aimed at killing prey microbes. Here, both the protein and small molecule cargo of the OMV and membrane fractions of M. xanthus were characterized and compared. Our analysis indicates a number of proteins that are OMV-specific or OMV-enriched, including several with putative hydrolytic function. Secondary metabolite profiling of OMVs identifies 16 molecules, many associated with antibiotic activities. Several hydrolytic enzyme homologs were identified, including MXAN_3564 (mepA, an M36 protease homolog. Genetic disruption of mepA leads to a significant reduction in extracellular protease activity suggesting MepA is the long-predicted (yet to date unknown primary extracellular protease in M. xanthus.

  4. El sistema de cargos en San Bernardino de Milpillas Chico, Durango

    Directory of Open Access Journals (Sweden)

    Chantal Cramaussel

    2013-01-01

    Full Text Available La principal estructura política de los tepehuanos es el sistema de cargos. En San Bernardino de Milpillas, Durango, los cargos de la Iglesia son diferentes a los del gobierno tradicional. Los segundos parecen apegarse más a las culturales locales, aunque los cargos de la Iglesia han sido objeto de transformaciones significativas para excluir a los ministros católicos en las ceremonias cristianas. La fiesta de la Candelaria, que precede a la toma de posesión de los nuevos “empleados”, muestra que los aspectos civiles de los cargos son indisociables de los rituales religiosos. Los cargos reunían funciones militares, judiciales y económicas, pero éstas han ido perdiendo vigencia por la violencia y por la compañía forestal, una notable fuente de recursos. Sin embargo, por el sistema de cargos los mestizos de Milpillas se han integrado a la organización política y la cultura de los tepehuanos.

  5. Irradiation Effects for the Pulsed Fast Neutron Analysis (PFNA) Cargo Interrogation System

    Energy Technology Data Exchange (ETDEWEB)

    Slater, C.O.

    2001-02-02

    At the request of Safety and Ecology Corporation of Tennessee, radiation effects of the proposed Pulsed Fast Neutron Analysis (PFNA) Cargo Interrogation System have been examined. First, fissile cargo were examined to determine if a significant neutron signal would be observable during interrogation. Results indicated that ample multiplication would be seen for near critical bare targets. The water-reflected sphere showed relatively little multiplication. By implication, a fissile target shielded by hydrogenous cargo might not be detectable by neutron interrogation, particularly if reliance is placed on the neutron signal. The cargo may be detectable if use can be made of the ample increase in the photon signal. Second, dose rates were calculated at various locations within and just outside the facility building. These results showed that some dose rates may be higher than the target dose rate of 0.05 mrem/h. However, with limited exposure time, the total dose may be well below the allowed total dose. Lastly, estimates were made of the activation of structures and typical cargo. Most cargo will not be exposed long enough to be activated to levels of concern. On the other hand, portions of the structure may experience buildup of some radionuclides to levels of concern.

  6. Air cargo market outlook and impact via the NASA CLASS project. [Cargo/Logistics Airlift Systems Study

    Science.gov (United States)

    Winston, M. M.; Conner, D. W.

    1980-01-01

    An overview is given of the Cargo/Logistics Airlift Systems Study (CLASS) project which was a 10 man-year effort carried out by two contractor teams, aimed at defining factors impacting future system growth and obtaining market requirements and design guidelines for future air freighters. Growth projection was estimated by two approaches: one, an optimal systems approach with a more efficient and cost effective system considered as being available in 1990; and the other, an evolutionary approach with an econometric behavior model used to predict long term evolution from the present system. Both approaches predict significant growth in demand for international air freighter services and less growth for U.S. domestic services. Economic analysis of air freighter fleet options indicate very strong market appeal of derivative widebody transports in 1990 with little incentive to develop all new dedicated air freighters utilizing the 1990's technology until sometime beyond the year 2000. Advanced air freighters would be economically attractive for a wide range of payload sizes (to 500 metric tons), however, if a government would share in the RD and T costs by virtue of its needs for a slightly modified version of a civil air freighter design (a.g. military airlifter).

  7. Large area x-ray detectors for cargo radiography

    Science.gov (United States)

    Bueno, C.; Albagli, D.; Bendahan, J.; Castleberry, D.; Gordon, C.; Hopkins, F.; Ross, W.

    2007-04-01

    Large area x-ray detectors based on phosphors coupled to flat panel amorphous silicon diode technology offer significant advances for cargo radiologic imaging. Flat panel area detectors provide large object coverage offering high throughput inspections to meet the high flow rate of container commerce. These detectors provide excellent spatial resolution when needed, and enhanced SNR through low noise electronics. If the resolution is reduced through pixel binning, further advances in SNR are achievable. Extended exposure imaging and frame averaging enables improved x-ray penetration of ultra-thick objects, or "select-your-own" contrast sensitivity at a rate many times faster than LDAs. The areal coverage of flat panel technology provides inherent volumetric imaging with the appropriate scanning methods. Flat panel area detectors have flexible designs in terms of electronic control, scintillator selection, pixel pitch, and frame rates. Their cost is becoming more competitive as production ramps up for the healthcare, nondestructive testing (NDT), and homeland protection industries. Typically used medical and industrial polycrystalline phosphor materials such as Gd2O2S:Tb (GOS) can be applied to megavolt applications if the phosphor layer is sufficiently thick to enhance x-ray absorption, and if a metal radiator is used to augment the quantum detection efficiency and reduce x-ray scatter. Phosphor layers ranging from 0.2-mm to 1-mm can be "sandwiched" between amorphous silicon flat panel diode arrays and metal radiators. Metal plates consisting of W, Pb or Cu, with thicknesses ranging from 0.25-mm to well over 1-mm can be used by covering the entire area of the phosphor plate. In some combinations of high density metal and phosphor layers, the metal plate provides an intensification of 25% in signal due to electron emission from the plate and subsequent excitation within the phosphor material. This further improves the SNR of the system.

  8. Overview of Crane Control Systems and the Related Problems: Analysis of Container Oscillation Using Different Types of Cargoes

    OpenAIRE

    Tomas Eglynas; Marijonas Bogdevičius; Arūnas Andziulis; Tomas Lenkauskas

    2016-01-01

    Growing international trading increased cargo transportation in containers, therefore the port cranes have higher loads. Increased cargo flows can influence transportation safety. It is therefore necessary to review the crane systems and determine what factors might influence the volatility of the container and its cargo during transportation. The paper includes consideration and analysis of crane control systems and related problems. The authors consider the reasons of problems, probable dam...

  9. Effect of process-dependent parameters of the handling-and-storage facility operation on the cargo handling cost

    OpenAIRE

    Шраменко, Наталя Юріївна

    2015-01-01

    Analysis of the handling-and-storage facility operation revealed the disadvantages of the technology that is related to the non-consideration of incoming cargo traffic variations in the planning and organization of industrial activity.The criterion of the handling-and-storage facility operation that presents unit costs for cargo handling has been formalized. The criterion makes it possible to take into account the cargo owner's costs associated with downtime of vehicles waiting for service an...

  10. 46 CFR 30.25-1 - Cargoes carried in vessels certificated under the rules of this subchapter.

    Science.gov (United States)

    2010-10-01

    ... category C oil-like NLS, under 33 CFR part 151. Requirements for category D NLS cargoes and mixtures of non-NLS cargoes with category D NLS cargoes are in 33 CFR part 151. Table 30.25-1—List of Flammable and..., n.o.s. (see also Oil, edible) D Including: Beechnut oil Castor oil Cocoa butter Coconut oil Corn...

  11. Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein

    OpenAIRE

    Yang LIU; Salter, Hannah K.; Holding, Andrew N.; Johnson, Christopher M.; Stephens, Elaine; Lukavsky, Peter J.; Walshaw, John; Bullock, Simon L.

    2013-01-01

    Cargo transport by the microtubule motor dynein is critical to diverse cellular processes. However, little is known about how different cargos are recognized and linked to this motor complex. Here, Liu et al. investigate the cargo-binding region of the dynein adaptor BicD in Drosophila. They provide a crystal structure of BicD and identify a shared binding site for two cargo-associated proteins: Rab6 and the RNA-binding protein Egl. This study provides structural insight into the molecular in...

  12. Cargo binding promotes KDEL receptor clustering at the mammalian cell surface

    Science.gov (United States)

    Becker, Björn; Shaebani, M. Reza; Rammo, Domenik; Bubel, Tobias; Santen, Ludger; Schmitt, Manfred J.

    2016-01-01

    Transmembrane receptor clustering is a ubiquitous phenomenon in pro- and eukaryotic cells to physically sense receptor/ligand interactions and subsequently translate an exogenous signal into a cellular response. Despite that receptor cluster formation has been described for a wide variety of receptors, ranging from chemotactic receptors in bacteria to growth factor and neurotransmitter receptors in mammalian cells, a mechanistic understanding of the underlying molecular processes is still puzzling. In an attempt to fill this gap we followed a combined experimental and theoretical approach by dissecting and modulating cargo binding, internalization and cellular response mediated by KDEL receptors (KDELRs) at the mammalian cell surface after interaction with a model cargo/ligand. Using a fluorescent variant of ricin toxin A chain as KDELR-ligand (eGFP-RTAH/KDEL), we demonstrate that cargo binding induces dose-dependent receptor cluster formation at and subsequent internalization from the membrane which is associated and counteracted by anterograde and microtubule-assisted receptor transport to preferred docking sites at the plasma membrane. By means of analytical arguments and extensive numerical simulations we show that cargo-synchronized receptor transport from and to the membrane is causative for KDELR/cargo cluster formation at the mammalian cell surface. PMID:27353000

  13. Delivery of Non-Native Cargo into Mammalian Cells Using Anthrax Lethal Toxin.

    Science.gov (United States)

    Rabideau, Amy E; Pentelute, Bradley Lether

    2016-06-17

    The intracellular delivery of peptide and protein therapeutics is a major challenge due to the plasma membrane, which acts as a barrier between the extracellular environment and the intracellular milieu. Over the past two decades, a nontoxic PA/LFN delivery platform derived from anthrax lethal toxin has been developed for the transport of non-native cargo into the cytosol of cells in order to understand the translocation process through a protective antigen (PA) pore and to probe intracellular biological functions. Enzyme-mediated ligation using sortase A and native chemical ligation are two facile methods used to synthesize these non-native conjugates, inaccessible by recombinant technology. Cargo molecules that translocate efficiently include enzymes from protein toxins, antibody mimic proteins, and peptides of varying lengths and non-natural amino acid compositions. The PA pore has been found to effectively convey over 30 known cargos other than native lethal factor (LF; i.e., non-native) with diverse sequences and functionalities on the LFN transporter protein. All together these studies demonstrated that non-native cargos must adopt an unfolded or extended conformation and contain a suitable charge composition in order to efficiently pass through the PA pore. This review provides insight into design parameters for the efficient delivery of new cargos using PA and LFN. PMID:27055654

  14. A proposed benchmark problem for cargo nuclear threat monitoring

    International Nuclear Information System (INIS)

    There is currently a great deal of technical and political effort focused on reducing the risk of potential attacks on the United States involving radiological dispersal devices or nuclear weapons. This paper proposes a benchmark problem for gamma-ray and X-ray cargo monitoring with results calculated using MCNP5, v1.51. The primary goal is to provide a benchmark problem that will allow researchers in this area to evaluate Monte Carlo models for both speed and accuracy in both forward and inverse calculational codes and approaches for nuclear security applications. A previous benchmark problem was developed by one of the authors (RPG) for two similar oil well logging problems (Gardner and Verghese, 1991, ). One of those benchmarks has recently been used by at least two researchers in the nuclear threat area to evaluate the speed and accuracy of Monte Carlo codes combined with variance reduction techniques. This apparent need has prompted us to design this benchmark problem specifically for the nuclear threat researcher. This benchmark consists of conceptual design and preliminary calculational results using gamma-ray interactions on a system containing three thicknesses of three different shielding materials. A point source is placed inside the three materials lead, aluminum, and plywood. The first two materials are in right circular cylindrical form while the third is a cube. The entire system rests on a sufficiently thick lead base so as to reduce undesired scattering events. The configuration was arranged in such a manner that as gamma-ray moves from the source outward it first passes through the lead circular cylinder, then the aluminum circular cylinder, and finally the wooden cube before reaching the detector. A 2 in.x4 in.x16 in. box style NaI (Tl) detector was placed 1 m from the point source located in the center with the 4 in.x16 in. side facing the system. The two sources used in the benchmark are 137Cs and 235U.

  15. A proposed benchmark problem for cargo nuclear threat monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Holmes, Thomas Wesley, E-mail: twholmes@ncsu.edu [Center for Engineering Applications of Radioisotopes, Nuclear Engineering Department, North Carolina State University, Raleigh, NC 27695-7909 (United States); Calderon, Adan; Peeples, Cody R.; Gardner, Robin P. [Center for Engineering Applications of Radioisotopes, Nuclear Engineering Department, North Carolina State University, Raleigh, NC 27695-7909 (United States)

    2011-10-01

    There is currently a great deal of technical and political effort focused on reducing the risk of potential attacks on the United States involving radiological dispersal devices or nuclear weapons. This paper proposes a benchmark problem for gamma-ray and X-ray cargo monitoring with results calculated using MCNP5, v1.51. The primary goal is to provide a benchmark problem that will allow researchers in this area to evaluate Monte Carlo models for both speed and accuracy in both forward and inverse calculational codes and approaches for nuclear security applications. A previous benchmark problem was developed by one of the authors (RPG) for two similar oil well logging problems (Gardner and Verghese, 1991, ). One of those benchmarks has recently been used by at least two researchers in the nuclear threat area to evaluate the speed and accuracy of Monte Carlo codes combined with variance reduction techniques. This apparent need has prompted us to design this benchmark problem specifically for the nuclear threat researcher. This benchmark consists of conceptual design and preliminary calculational results using gamma-ray interactions on a system containing three thicknesses of three different shielding materials. A point source is placed inside the three materials lead, aluminum, and plywood. The first two materials are in right circular cylindrical form while the third is a cube. The entire system rests on a sufficiently thick lead base so as to reduce undesired scattering events. The configuration was arranged in such a manner that as gamma-ray moves from the source outward it first passes through the lead circular cylinder, then the aluminum circular cylinder, and finally the wooden cube before reaching the detector. A 2 in.x4 in.x16 in. box style NaI (Tl) detector was placed 1 m from the point source located in the center with the 4 in.x16 in. side facing the system. The two sources used in the benchmark are {sup 137}Cs and {sup 235}U.

  16. A proposed benchmark problem for cargo nuclear threat monitoring

    Science.gov (United States)

    Wesley Holmes, Thomas; Calderon, Adan; Peeples, Cody R.; Gardner, Robin P.

    2011-10-01

    There is currently a great deal of technical and political effort focused on reducing the risk of potential attacks on the United States involving radiological dispersal devices or nuclear weapons. This paper proposes a benchmark problem for gamma-ray and X-ray cargo monitoring with results calculated using MCNP5, v1.51. The primary goal is to provide a benchmark problem that will allow researchers in this area to evaluate Monte Carlo models for both speed and accuracy in both forward and inverse calculational codes and approaches for nuclear security applications. A previous benchmark problem was developed by one of the authors (RPG) for two similar oil well logging problems (Gardner and Verghese, 1991, [1]). One of those benchmarks has recently been used by at least two researchers in the nuclear threat area to evaluate the speed and accuracy of Monte Carlo codes combined with variance reduction techniques. This apparent need has prompted us to design this benchmark problem specifically for the nuclear threat researcher. This benchmark consists of conceptual design and preliminary calculational results using gamma-ray interactions on a system containing three thicknesses of three different shielding materials. A point source is placed inside the three materials lead, aluminum, and plywood. The first two materials are in right circular cylindrical form while the third is a cube. The entire system rests on a sufficiently thick lead base so as to reduce undesired scattering events. The configuration was arranged in such a manner that as gamma-ray moves from the source outward it first passes through the lead circular cylinder, then the aluminum circular cylinder, and finally the wooden cube before reaching the detector. A 2 in.×4 in.×16 in. box style NaI (Tl) detector was placed 1 m from the point source located in the center with the 4 in.×16 in. side facing the system. The two sources used in the benchmark are 137Cs and 235U.

  17. pH-Dependent Assembly and Segregation of the Coiled-Coil Segments of Yeast Putative Cargo Receptors Emp46p and Emp47p

    OpenAIRE

    Kentaro Ishii; Hiroki Enda; Masanori Noda; Megumi Kajino; Akemi Kim; Eiji Kurimoto; Ken Sato; Akihiko Nakano; Yuji Kobayashi; Hirokazu Yagi; Susumu Uchiyama; Koichi Kato

    2015-01-01

    Emp46p and Emp47p are yeast putative cargo receptors that recycle between the endoplasmic reticulum and the Golgi apparatus. These receptors can form complexes in a pH-dependent manner, but their molecular mechanisms remain unclear. Here, we successfully reproduced their interactions in vitro solely with their coiled-coil segments, which form stable heterotetramers in the neutral condition but segregate at lower pH. Mutational data identified a key glutamate residue of Emp46p that serves as t...

  18. Synthesis and characterization of nanowire coils of organometallic coordination polymers for controlled cargo release.

    Science.gov (United States)

    Liang, Guodong; Ni, Huan; Bao, Suping; Zhu, Fangming; Gao, Haiyang; Wu, Qing

    2014-06-12

    Nanowire coils of organometallic coordination polymers have been synthesized for the first time by using the emulsion periphery polymerization technique. An amphiphilic triblock copolymer terminated with inclusion complex of β-cyclodextrin and 4,4'-bipyridine self-assembles into oil-in-water emulsion in a toluene/water mixture. Subsequent coordination of bipyridine with Ni(II) in periphery of emulsions results in the formation of coordination polymer nanowire coils. The nanowire coils are composed of nanowires with diameter of 2 nm. Nanowire coils exhibit enhanced thermal stability in contrast to their parent triblock copolymer. Interestingly, nanowire coils are capable of encapsulating organic cargoes. Encapsulated cargoes can be selectively extracted from nanowire coils without damaging nanowire coils. Nanowire coils are potential candidates for encapsulating and controlled release of organic cargoes. PMID:24842771

  19. Tackling the x-ray cargo inspection challenge using machine learning

    Science.gov (United States)

    Jaccard, Nicolas; Rogers, Thomas W.; Morton, Edward J.; Griffin, Lewis D.

    2016-05-01

    The current infrastructure for non-intrusive inspection of cargo containers cannot accommodate exploding com-merce volumes and increasingly stringent regulations. There is a pressing need to develop methods to automate parts of the inspection workflow, enabling expert operators to focus on a manageable number of high-risk images. To tackle this challenge, we developed a modular framework for automated X-ray cargo image inspection. Employing state-of-the-art machine learning approaches, including deep learning, we demonstrate high performance for empty container verification and specific threat detection. This work constitutes a significant step towards the partial automation of X-ray cargo image inspection.

  20. Fast-neutron/gamma-ray radiography scanner for the detection of contraband in air cargo containers

    Science.gov (United States)

    Eberhardt, J.; Liu, Y.; Rainey, S.; Roach, G.; Sowerby, B.; Stevens, R.; Tickner, J.

    2006-05-01

    There is a worldwide need for efficient inspection of cargo containers at airports, seaports and road border crossings. The main objectives are the detection of contraband such as illicit drugs, explosives and weapons. Due to the large volume of cargo passing through Australia's airports every day, it is critical that any scanning system should be capable of working on unpacked or consolidated cargo, taking at most 1-2 minutes per container. CSIRO has developed a fast-neutron/gamma-ray radiography (FNGR) method for the rapid screening of air freight. By combining radiographs obtained using 14 MeV neutrons and 60Co gamma-rays, high resolution images showing both density and material composition are obtained. A near full-scale prototype scanner has been successfully tested in the laboratory. With the support of the Australian Customs Service, a full-scale scanner has recently been installed and commissioned at Brisbane International Airport.

  1. Microbial influenced corrosion in cargo oil tanks of crude oil tankers

    Energy Technology Data Exchange (ETDEWEB)

    Huang, R.T. [Chevron Shipping Co., San Francisco, CA (United States); McFarland, B.L. [Chevron Research and Technology Co., Richmond, CA (United States); Hodgman, R.Z. [Corrpro Companies Inc., Hayward, CA (United States)

    1997-09-01

    Excessive pitting corrosion in the uncoated bottom platings of cargo oil tanks was detected in newbuilt crude oil tankers only 2 to 5 years old, and was diagnosed as microbial influenced corrosion (MIC). Sulfate-reducing bacteria (SRB) concentrations reached as high as 100,000 to 10,000,000 per milliliter in the settled water at the bottom of the cargo oil tanks. Biocide treatment to control MIC was studied in the laboratory using microbial consortia isolated from cargo oil tank bottoms. Biocide treatment to control MIC was attempted experimentally, but was found to be impractical onboard tankers. A MIC mitigation strategy, employing an enhanced pitting inspection and repair program combined with the coating of the bottom platings, was developed and implemented for existing crude oil tankers. Considerations for improvement of future newbuild crude oil tankers are also presented.

  2. GSK-3 regulates transport of kinesin-1 driven cargos in vivo

    Science.gov (United States)

    Leidel, Christina; Weaver, Carole; Szpankowski, Lukasz; Goldstein, Lawrence S. B.; Shubeita, George T.; CenterNonlinear Dynamics, Department of Physics, University of Texas At Austin Collaboration; Hhmi, Department of Cellular; Molecular Medicine, Univ. Of California Collaboration

    2011-03-01

    The Glycogen Synthase Kinase 3 (GSK-3) has been linked to many aspects of the development of Alzheimer's disease and was proposed to play a role in the transport of the Amyloid Precursor Protein (APP) by kinesin-1 motors. Using Drosophila embryos and larvae with altered GSK-3 expression, we characterize motor transport of cargos including APP and lipid droplets using DIC microscopy, high-resolution video tracking, fluorescence, and in vivo stall force measurements with optical tweezers. By comparing cargo velocities and run lengths we find that GSK-3 is a required negative regulator of in vivo transport. Stall force measurements on lipid droplets reveal that enhanced transport under conditions of reduced GSK-3 is a result of a larger number of active motors hauling the cargo. Our findings have implications on the use of GSK-3 inhibitors in treatment of Alzheimer's disease.

  3. Dry bulk cargo shipping - An overlooked threat to the marine environment?

    Science.gov (United States)

    Grote, Matthias; Mazurek, Nicole; Gräbsch, Carolin; Zeilinger, Jana; Le Floch, Stéphane; Wahrendorf, Dierk-Steffen; Höfer, Thomas

    2016-09-15

    Approximately 9.5billiontonnes of goods is transported over the world oceans annually with dry bulk representing the largest cargo group. This paper aims to analyse whether the transport and associated inputs of dry bulks into the sea create a risk for the marine environment. For this purpose, we analyse the international regulatory background concerning environmental protection (MARPOL), estimate quantities and identify inputs of such cargoes into the oceans (accidental and operational), and use available information for hazard assessment. Annually, more than 2.15milliontonnes of dry bulk cargoes are likely to enter the oceans, of which 100,000tonnes are potentially harmful to the marine environment according to the definition included in draft maritime regulation. The assessment of the threat to the marine environment is hampered by a lack of available information on chemical composition, bioavailability and toxicity. Perspectives for amendments of the unsatisfying pollution prevention regulations are discussed. PMID:27339744

  4. Electrostatic assembly/disassembly of nanoscaled colloidosomes for light-triggered cargo release

    KAUST Repository

    Li, Song

    2015-04-27

    Colloidosome capsules possess the potential for the encapsulation and release of molecular and macromolecular cargos. However, the stabilization of the colloidosome shell usually requires an additional covalent crosslinking which irreversibly seals the capsules, and greatly limits their applications in large-cargos release. Herein we report nanoscaled colloidosomes designed by the electrostatic assembly of organosilica nanoparticles (NPs) with oppositely charged surfaces (rather than covalent bonds), arising from different contents of a bridged nitrophenylene-alkoxysilane [NB; 3-nitro-N-(3-(triethoxysilyl)propyl)-4-(((3-(triethoxysilyl)propyl)-amino)methyl)benzamid] derivative in the silica. The surface charge of the positively charged NPs was reversed by light irradiation because of a photoreaction in the NB moieties, which impacted the electrostatic interactions between NPs and disassembled the colloidosome nanosystems. This design was successfully applied for the encapsulation and light-triggered release of cargos. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Applicability of gamma detection systems for the detection of illicit transports of radioactive substances in cargo containers shielded by the cargo

    International Nuclear Information System (INIS)

    The topic of this research project is the detection of illicit trafficking of radioactive material inside of cargo containers. The currently used radiation portal monitors (RPM) at border crossings are not sufficient due to two effects which often occur during operation: false positive and false negative alarms. In case of false positive an alarm is given in the absence of radioactive material (or natural and harmless radioactive substances are classified as relevant anthropogenic material). In the second case, false negative, radioactive material with high risk potential is not detected. Because of these two effects, more advanced detections systems and other technical efforts are desirable. The expected increase of cargo container flow and special security programs like the Mega Port Initiative are demanding more reliable detection systems. Main focus of research project: This research project focuses on the applicability of spectrometric gamma detection systems in general. Regardless whether currently used, advanced detectors or hypothetic gamma detectors, all need a certain amount of gamma radiation to afford the identification of characteristic peaks. The overall problem is that the emitted gamma radiation is attenuated and has to be discriminated from the radiation background. One has to consider that the attenuation of radiation depends on the energy, and that often in the lower part of the energetic spectrum a higher background occurs. This leads in particular to a larger decrease of detection probability of radionuclides in the lower part of the energy spectrum. One can assume that even with high ambitioned efforts only a small progress in detection efficiency will be achieved because the marginal improvements approach physical limitations that cannot be overcome. The first part of the research project consisted of measurements with portal monitors and, in comparison, smaller spectrometric detectors. This was done in order to get an overview on realistic

  6. Gamma-ray and neutron radiography for a pulsed fast neutron analysis cargo inspection system

    International Nuclear Information System (INIS)

    This paper presents the design, optimization, and characterization of a gamma-ray and neutron radiographic subsystem that was developed for the Pulsed Fast Neutron Analysis (PFNA) cargo inspection system. The PFNA inspection system uses D-D based nanosecond pulsed neutron source to produce three-dimensional elemental content images of cargo. The PFNA neutron source produces gamma rays as well as neutrons. The new radiographic subsystem measures these radiations in an array of plastic scintillators to produce gamma-ray and neutron transmission images of the cargo simultaneously with the PFNA elemental content measurement. Although the radiographic subsystem improves PFNA performance in many forms of contraband detection, it was specifically designed to detect Special Nuclear Material (SNM) in cargo containers and trucks. A feasibility study, including experiments and modeling, was performed to determine the usefulness of neutron and gamma-ray radiography in this application. The study showed that the baseline configuration was useful in cargoes up to 144 g/cm2 thick. In order to improve the subsystem performance, the source output needed to be increased. The neutron and gamma-ray yield and spectrum were measured for a variety of different beam stops. The maximum cargo thickness was increased to 180 g/cm2 by changing the source beam stop from gold to copper and by increasing the detector length to 17.0 cm. An experiment was then performed that determined a 3.5 cm radiographic resolution was adequate for SNM detection. The detector configuration and the source motion were optimized to obtain a resolution of approximately 3.5 cm using the minimal number of detectors (128) and the maximum detector diameter (5.1 cm). A prototype of the final design was built, installed, and tested, and is currently in use at the PFNA test facility. (author)

  7. Accelerator requirements for fast-neutron interrogation of luggage and cargo

    International Nuclear Information System (INIS)

    Several different fast-neutron based techniques are being studied for the detection of contraband substances in luggage and cargo containers. The present work discusses the accelerator requirements for fast-neutron transmission spectroscopy (FNTS), pulsed fast-neutron analysis (PFNA), and 14-MeV neutron interrogation. These requirements are based on the results of Monte-Carlo simulations of neutron or gamma detection rates. Accelerator requirements are driven by count-rate considerations, spatial resolution and acceptable uncertainties in elemental compositions. The authors have limited their analyses to luggage inspection with FNTS and to cargo inspection with PFNA or 14-MeV neutron interrogation

  8. Enabling a viable technique for the optimization of LNG carrier cargo operations

    Science.gov (United States)

    Alaba, Onakoya Rasheed; Nwaoha, T. C.; Okwu, M. O.

    2016-07-01

    In this study, we optimize the loading and discharging operations of the Liquefied Natural Gas (LNG) carrier. First, we identify the required precautions for LNG carrier cargo operations. Next, we prioritize these precautions using the analytic hierarchy process (AHP) and experts' judgments, in order to optimize the operational loading and discharging exercises of the LNG carrier, prevent system failure and human error, and reduce the risk of marine accidents. Thus, the objective of our study is to increase the level of safety during cargo operations.

  9. CargoVibes: human response to vibration due to freight rail traffic

    OpenAIRE

    Waddington, D.; Woodcock, J.; Smith, M.G.; Janssen, S.A.; Persson Waye, K.

    2015-01-01

    The aim of this paper is to present an overview of the research concerning human response to vibration conducted in the EU FP7 CargoVibes project. The European Union-funded project CargoVibes involved 10 partners from 8 nations and ran from April 2011 to April 2014. The project was concerned with railway-induced ground-borne vibration affecting residents close to freight lines, with one work package that investigated human response to vibration, including sleep disturbance, community annoyanc...

  10. Genetic algorithms approach for containerships fleet management dependent on cargo and their deadlines

    OpenAIRE

    Moura, Ana; Martins, Paulo; Andrade-Campos, António; Lobo,

    2010-01-01

    This work proposes a method to improve the flexibility of short sea shipping and to increase its competitiveness with other means of freight transport. A logistic model and a mathematical model are developed to manage a fleet of two or more vessels which transport cargo to and from several ports, bearing in mind the cargo distribution and delivery deadlines. At each port, the model determines which port to visit next, which containers to embark, disembark and to shift, as well as how to stow ...

  11. Research on combination forecast of port cargo throughput based on time series and causality analysis

    OpenAIRE

    Chi Zhang; Lei Huang; Zhichao Zhao

    2013-01-01

    Purpose: The purpose of this paper is to develop a combined model composed of grey-forecast model and Logistic-growth-curve model to improve the accuracy of forecast model of cargo throughput for the port. The authors also use the existing data of a current port to verify the validity of the combined model.Design/methodology/approach: A literature review is undertaken to find the appropriate forecast model of cargo throughput for the port. Through researching the related forecast model, the a...

  12. Protocells and their use for targeted delivery of multicomponent cargos to cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Brinker, C Jeffrey; Ashley, Carlee Erin; Jiang, Xingmao; Liu, Juewen; Peabody, David S; Wharton, Walker Richard; Carnes, Eric; Chackerian, Bryce; Willman, Cheryl L

    2015-03-31

    Various embodiments provide materials and methods for synthesizing protocells for use in targeted delivery of cargo components to cancer cells. In one embodiment, the lipid bilayer can be fused to the porous particle core to form a protocell. The lipid bilayer can be modified with targeting ligands or other ligands to achieve targeted delivery of cargo components that are loaded within the protocell to a target cell, e.g., a type of cancer. Shielding materials can be conjugated to the surface of the lipid bilayer to reduce undesired non-specific binding.

  13. Air cargo: An Integrated Systems View. 1978 Summer Faculty Fellowship Program in Engineering Systems Design

    Science.gov (United States)

    Keaton, A. (Editor); Eastman, R. (Editor); Hargrove, A. (Editor); Rabiega, W. (Editor); Olsen, R. (Editor); Soberick, M. (Editor)

    1978-01-01

    The national air cargo system is analyzed and how it should be in 1990 is prescribed in order to operate successfully through 2015; that is through one equipment cycle. Elements of the system which are largely under control of the airlines and the aircraft manufacturers are discussed. The discussion deals with aircraft, networks, facilities, and procedures. The regulations which govern the movement of air freight are considered. The larger public policy interests which must be served by the kind of system proposed, the air cargo integrated system (ACIS), are addressed. The possible social, economical, political, and environment impacts of the system are considered. Recommendations are also given.

  14. Automatic air cargo selection and weight balancing: A mixed integer programming approach

    OpenAIRE

    Vancroonenburg, Wim; Verstichel, Jannes; Tavernier, Karel; Vanden Berghe, Greet

    2014-01-01

    The present contribution introduces a mixed integer linear programming model as a decision support tool for air cargo load planning. The main objective for the model is to find the most profitable selection from a set of cargo to be loaded on an aircraft. The secondary objective is to minimize the deviation between the aircraft’s centre of gravity, and a known target value so as to reduce fuel consumption and improve stability. The model is subject to a large number of constraints that ensure...

  15. Triple Emulsion Drops with An Ultrathin Water Layer: High Encapsulation Efficiency and Enhanced Cargo Retention in Microcapsules.

    Science.gov (United States)

    Choi, Chang-Hyung; Lee, Hyomin; Abbaspourrad, Alireza; Kim, June Hwan; Fan, Jing; Caggioni, Marco; Wesner, Chris; Zhu, Taotao; Weitz, David A

    2016-05-01

    Triple emulsion drops with an ultrathin water layer are developed to achieve high encapsulation efficiency of hydrophobic cargo in a hydrophobic polymeric shell, directly dispersed in water. Furthermore, enhanced retention of volatile hydrophobic cargo is achieved by forming a hydrogel network within this water layer that serves as a physical barrier. PMID:26932778

  16. 46 CFR 154.1836 - Vapor venting as a means of cargo tank pressure and temperature control.

    Science.gov (United States)

    2010-10-01

    ... temperature control. 154.1836 Section 154.1836 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... LIQUEFIED GASES Operations § 154.1836 Vapor venting as a means of cargo tank pressure and temperature... cargo pressure and temperature control system under §§ 154.701 through 154.709 is operating and...

  17. Overview of Crane Control Systems and the Related Problems: Analysis of Container Oscillation Using Different Types of Cargoes

    Directory of Open Access Journals (Sweden)

    Tomas Eglynas

    2016-04-01

    Full Text Available Growing international trading increased cargo transportation in containers, therefore the port cranes have higher loads. Increased cargo flows can influence transportation safety. It is therefore necessary to review the crane systems and determine what factors might influence the volatility of the container and its cargo during transportation. The paper includes consideration and analysis of crane control systems and related problems. The authors consider the reasons of problems, probable damage and solution methods. The paper also provides the analysis of the relationship between different container cargoes and container oscillations occurring during handling operations using a container crane prototype. The analysis of the effect of different cargoes in containers on loading process and the results of occurring oscillations are presented.

  18. 46 CFR 32.50-5 - Cargo pump gauges on tank vessels constructed on or after November 10, 1936-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo pump gauges on tank vessels constructed on or... SECURITY TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Pumps, Piping, and Hose for Cargo Handling § 32.50-5 Cargo pump gauges on tank vessels constructed on or after November 10, 1936—TB/ALL....

  19. 46 CFR Table 2 to Part 153 - Cargoes Not Regulated Under Subchapters D or O of This Chapter When Carried in Bulk on Non...

    Science.gov (United States)

    2010-10-01

    ... and mixtures of non-NLS cargoes with Category D NLS cargoes are in 33 CFR part 151. Cargoes Pollution... solution C Sodium sulfate solution III Sorbitol solution III Sulfonated polyacrylate solution III... an oceangoing vessel, see 46 CFR 153.900(c). —A NLS category in brackets indicates that the...

  20. Analysis of VGH data from two types of four-engine airplanes in commercial cargo service

    Science.gov (United States)

    Healy, F. M.

    1972-01-01

    Data are presented for derived gust velocities and for incremental normal accelerations due to gusts, maneuvers, and landing impacts. The data were obtained from NASA VGH recorders installed on three four-engine cargo airplanes operated by three airlines. Continental United States and trans-Pacific routes were covered.

  1. 29 CFR 1919.81 - Examination of bulk cargo loading or discharging spouts or suckers.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Examination of bulk cargo loading or discharging spouts or suckers. 1919.81 Section 1919.81 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) GEAR CERTIFICATION Certification of Shore-Based Material Handling Devices §...

  2. 78 FR 57485 - Security Zone; Protection of Military Cargo, Captain of the Port Zone Puget Sound

    Science.gov (United States)

    2013-09-19

    ... Puget Sound AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast... cargo in the navigable waters of Puget Sound and adjacent waters. Entry into this zone is prohibited... or email LTJG Johnny Zeng, Sector Puget Sound Waterways Management Division, Coast Guard;...

  3. 78 FR 50134 - Altus Pharmaceuticals, Inc., Blackhawk Capital Group BDC, Inc., Cargo Connection Logistics...

    Science.gov (United States)

    2013-08-16

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Altus Pharmaceuticals, Inc., Blackhawk Capital Group BDC, Inc., Cargo Connection Logistics Holding... securities of Altus Pharmaceuticals, Inc. because it has not filed any periodic reports since the...

  4. 77 FR 50926 - Security Zones; Certain Dangerous Cargo Vessels, Tampa, FL

    Science.gov (United States)

    2012-08-23

    ... DHS Department of Homeland Security CDC Certain Dangerous Cargo FR Federal Register NPRM Notice of..., which, due to their ] political, economic, social, or religious significance, may render them... Ombudsman evaluates these actions annually and rates each agency's responsiveness to small business. If...

  5. Functional description for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB)

    Energy Technology Data Exchange (ETDEWEB)

    Truett, L.F.; Rollow, J.P.; Shipe, P.C. [Oak Ridge National Lab., TN (United States); Faby, E.Z.; Fluker, J.; Hancock, W.R.; Grubb, J.W.; Russell, D.L. [Univ. of Tennessee, Knoxville, TN (United States); Ferguson, R.A. [SAIC, Oak Ridge, TN (United States)

    1995-12-15

    This Functional Description for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB) documents the purpose of and requirements for the ICDB in order to ensure a mutual understanding between the development group and the user group of the system. This Functional Description defines ICDB and provides a clear statement of the initial operational capability to be developed.

  6. 33 CFR 150.405 - How must a cargo transfer system be tested and inspected?

    Science.gov (United States)

    2010-07-01

    ... be tested and inspected? 150.405 Section 150.405 Navigation and Navigable Waters COAST GUARD... Operations § 150.405 How must a cargo transfer system be tested and inspected? (a) No person may transfer oil... and tested according to this section. (b) The single point mooring (SPM)-CTS must be maintained...

  7. Dragonair- Year-on-Year Rises Continue For Passengers And Cargo

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Passenger numbers at Dragonair rose 13.2% while cargo volumes climbed 23.9%in April compared with the same month in 2004. The airline flew 431,860 passengers last month, 50,274 more than one year ago,and 7.3% up on March.

  8. 33 CFR 157.37 - Discharge of oily mixtures from oil cargoes.

    Science.gov (United States)

    2010-07-01

    ... from such cargoes to a reception facility. (c) Each oil discharge monitoring and control system must be... discharge monitoring and control system must be retained for at least three years. The data for the most... the vessel is an existing vessel with a Part Flow System meeting that appendix; or (iii) Below...

  9. 75 FR 6092 - Special Conditions: Model C-27J Airplane; Class E Cargo Compartment Lavatory

    Science.gov (United States)

    2010-02-08

    ... Groves, FAA, International Branch, ANM-116, Transport Airplane Directorate, Aircraft Certification... that each cargo compartment must meet one of the Class requirements of Sec. 25.857; and the flight.... Two types of shut-off systems meet this requirement: A manual system, with an airplane flight...

  10. Gamma/x-ray linear pushbroom stereo for 3D cargo inspection

    Science.gov (United States)

    Zhu, Zhigang; Hu, Yu-Chi

    2006-05-01

    For evaluating the contents of trucks, containers, cargo, and passenger vehicles by a non-intrusive gamma-ray or X-ray imaging system to determine the possible presence of contraband, three-dimensional (3D) measurements could provide more information than 2D measurements. In this paper, a linear pushbroom scanning model is built for such a commonly used gamma-ray or x-ray cargo inspection system. Accurate 3D measurements of the objects inside a cargo can be obtained by using two such scanning systems with different scanning angles to construct a pushbroom stereo system. A simple but robust calibration method is proposed to find the important parameters of the linear pushbroom sensors. Then, a fast and automated stereo matching algorithm based on free-form deformable registration is developed to obtain 3D measurements of the objects under inspection. A user interface is designed for 3D visualization of the objects in interests. Experimental results of sensor calibration, stereo matching, 3D measurements and visualization of a 3D cargo container and the objects inside, are presented.

  11. 49 CFR 1544.202 - Persons and property onboard an all-cargo aircraft.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Persons and property onboard an all-cargo aircraft. 1544.202 Section 1544.202 Transportation Other Regulations Relating to Transportation (Continued... SECURITY: AIR CARRIERS AND COMMERCIAL OPERATORS Operations § 1544.202 Persons and property onboard an...

  12. Tracking 800-year-old Shipments: An Archaeological Investigation of the Mado Shipwreck Cargo, Taean, Korea

    Science.gov (United States)

    Kim, Minkoo; Moon, Whan Suk

    2011-12-01

    This paper examines cargo from an 800-year-old shipwreck and discusses its implications in relation to the exchange networks and maritime transportation of the Goryeo period (A.D. 918-1392) in Korea. In 2007, two local fishermen fortuitously discovered porcelain vessels from a Goryeo-period shipwreck off the mid-western coast of the Korean peninsula. Underwater excavation conducted by the National Research Institute of Maritime Cultural Heritage (NRIMCH) revealed that the ship was carrying a myriad of cargoes destined for Gaegyeong, the capital of the Goryeo dynasty. Excavation indicates that the main body of the cargo was porcelain vessels produced in the southern part of the peninsula. Archaeobotanical investigation of the wreck deposits revealed that the ship was carrying crops such as rice ( Oryza sativa L.), broomcorn millet ( Panicum miliaceum L.), foxtail millet ( Setaria italica (L.) P. Beauv.), and buckwheat ( Fagopyrum esculentum Moench) bound for the capital. Among the recovered objects were 73 wooden cargo tags with Chinese letters. These tags, equivalent to the modern day air bill, contained detailed information about the senders, recipients, and shipped goods. These findings indicate that during the Goryeo period maritime transportation played an important role in the interpersonal exchange of products over long distances.

  13. Conceptual design of the french MAPS NTP cargo shuttle based on a particle bed reactor

    International Nuclear Information System (INIS)

    MAPS, a 3-years study program on NTP has been launched at CEA in 1994 following the conclusions of a preliminary scoping study of an NTP system for earth to moon orbit cargo shuttle missions. This paper presents the main results obtained after one year of studies, and gives an outline of the future work. copyright 1996 American Institute of Physics

  14. The use of x-ray imaging technology in cargo inspection

    International Nuclear Information System (INIS)

    The aftermath of the September 11,2001 terrorist attack in the United States has brought a significant change in the conduct of trade and commerce among nations. The introduction on non-intrusive cargo inspection equipment, most x-ray and gamma ray scanners, among customs administrations worldwide has been widely employed and incorporated in their respective cargo examination procedures as part of their security initiative to deter terrorist threats. In compliance with our international commitment and agreement passed by the World Customs Organization which required the use of modern technology in cargo inspection, the Bureau of Customs had acquired 30 units of container x-ray scanners in 2006 from China that became operational in 2007 and the acquisition of which was guided by the following objectives: maximize collection of government revenues,facilitate trade and commerce, prevent smuggling, and safeguard the public and environment against the entry of hazardous and harmful substance. In this light, this paper discusses the brief background of the non-intrusive container inspection system project of the Bureau of Customs, an overview of the technical features of x-ray imaging technology acquired, the procedures in the application of x-ray inspection in containerized cargo, the radiation safety infrastructures and considerations, and a review of some major accomplishments after its two years of existence. (author)

  15. 77 FR 44528 - Dry Cargo Residue Discharges in the Great Lakes

    Science.gov (United States)

    2012-07-30

    ... requesting information about the then-current status of dry cargo operations in the Great Lakes (69 FR 77147... the January 17, 2008, issue of the Federal Register (73 FR 3316). D. Public Meeting We do not plan to... Protection Agency FEIS Final Environmental Impact Statement FR Federal Register ICR Information...

  16. Endocytic Trafficking of Membrane-Bound Cargo: A Flotillin Point of View

    Directory of Open Access Journals (Sweden)

    Melanie Meister

    2014-07-01

    Full Text Available The ubiquitous and highly conserved flotillin proteins, flotillin-1 and flotillin-2, have been shown to be involved in various cellular processes such as cell adhesion, signal transduction through receptor tyrosine kinases as well as in cellular trafficking pathways. Due to the fact that flotillins are acylated and form hetero-oligomers, they constitutively associate with cholesterol-enriched lipid microdomains. In recent years, such microdomains have been appreciated as platforms that participate in endocytosis and other cellular trafficking steps. This review summarizes the current findings on the role of flotillins in membrane-bound cargo endocytosis and endosomal trafficking events. We will discuss the proposed function of flotillins in endocytosis in the light of recent findings that point towards a role for flotillins in a step that precedes the actual endocytic uptake of cargo molecules. Recent findings have also revealed that flotillins may be important for endosomal sorting and recycling of specific cargo molecules. In addition to these aspects, the cellular trafficking pathway of flotillins themselves as potential cargo in the context of growth factor signaling will be discussed.

  17. 19 CFR 122.48a - Electronic information for air cargo required in advance of arrival.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Electronic information for air cargo required in... OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Aircraft Entry and Entry Documents; Electronic Manifest Requirements for Passengers, Crew Members, and Non-Crew Members...

  18. 19 CFR 192.14 - Electronic information for outward cargo required in advance of departure.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Electronic information for outward cargo required in advance of departure. 192.14 Section 192.14 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) EXPORT CONTROL Filing of Export Information Through the Automated Export...

  19. 77 FR 25187 - Extension of Agency Information Collection Activity Under OMB Review: Certified Cargo Screening...

    Science.gov (United States)

    2012-04-27

    ... and include facilties upstream ] in the air cargo supply chain, such as shippers, manufacturers, warehousing entities, distributors, third party logistics companies, and indirect air carriers located in the... collection of information on February 24, 2012, 77 FR 11146, and TSA received no comments. The...

  20. 23 CFR 658.23 - LCV freeze; cargo-carrying unit freeze.

    Science.gov (United States)

    2010-04-01

    ... including the truck tractor) whose cargo-carrying units exceed: (i) The maximum combination trailer, semitrailer, or other type of length limitation authorized by State law or regulation of that State on or... units may operate on the NN. (1) Truck tractor-semitrailer-trailer and truck...

  1. 46 CFR 308.548 - Standard form of underwriting agency agreement for cargo, Form MA-318.

    Science.gov (United States)

    2010-10-01

    ... agreements executed by the Maritime Administrator and domestic insurance companies authorized to do a marine insurance business in any State of the United States, appointing such companies as Underwriting Agents to... EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Cargo Insurance Iv-General § 308.548 Standard form...

  2. The Targeted Delivery of Multicomponent Cargos to Cancer Cells via Nanoporous Particle-Supported Lipid Bilayers

    OpenAIRE

    Ashley, Carlee E.; CARNES, ERIC C.; Phillips, Genevieve K.; Padilla, David; Durfee, Paul N.; Brown, Page A.; Hanna, Tracey N.; Liu, Juewen; Phillips, Brandy; Carter, Mark B.; Carroll, Nick J.; Jiang, Xingmao; Dunphy, Darren R.; Willman, Cheryl L.; Petsev, Dimiter N.

    2011-01-01

    Encapsulation of drugs within nanocarriers that selectively target malignant cells promises to mitigate side effects of conventional chemotherapy and to enable delivery of the unique drug combinations needed for personalized medicine. To realize this potential, however, targeted nanocarriers must simultaneously overcome multiple challenges, including specificity, stability, and a high capacity for disparate cargos. Here we report porous nanoparticle-supported lipid bilayers (protocells) that ...

  3. Database specification for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB)

    Energy Technology Data Exchange (ETDEWEB)

    Faby, E.Z.; Fluker, J.; Hancock, B.R.; Grubb, J.W.; Russell, D.L. [Univ. of Tennessee, Knoxville, TN (United States); Loftis, J.P.; Shipe, P.C.; Truett, L.F. [Oak Ridge National Lab., TN (United States)

    1994-03-01

    This Database Specification for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB) describes the database organization and storage allocation, provides the detailed data model of the logical and physical designs, and provides information for the construction of parts of the database such as tables, data elements, and associated dictionaries and diagrams.

  4. 33 CFR 155.310 - Containment of oil and hazardous material cargo discharges.

    Science.gov (United States)

    2010-07-01

    ...) For a tankship, as defined in 46 CFR 30.10-67, the coaming or other barrier required in 46 CFR 32.56... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Containment of oil and hazardous... FOR VESSELS Vessel Equipment § 155.310 Containment of oil and hazardous material cargo discharges....

  5. A whole-system approach to x-ray spectroscopy in cargo inspection systems

    Science.gov (United States)

    Langeveld, Willem G. J.; Gozani, Tsahi; Ryge, Peter; Sinha, Shrabani; Shaw, Tim; Strellis, Dan

    2013-04-01

    The bremsstrahlung x-ray spectrum used in high-energy, high-intensity x-ray cargo inspection systems is attenuated and modified by the materials in the cargo in a Z-dependent way. Therefore, spectroscopy of the detected x rays yields information about the Z of the x-rayed cargo material. It has previously been shown that such ZSpectroscopy (Z-SPEC) is possible under certain circumstances. A statistical approach, Z-SCAN (Z-determination by Statistical Count-rate ANalysis), has also been shown to be effective, and it can be used either by itself or in conjunction with Z-SPEC when the x-ray count rate is too high for individual x-ray spectroscopy. Both techniques require fast x-ray detectors and fast digitization electronics. It is desirable (and possible) to combine all techniques, including x-ray imaging of the cargo, in a single detector array, to reduce costs, weight, and overall complexity. In this paper, we take a whole-system approach to x-ray spectroscopy in x-ray cargo inspection systems, and show how the various parts interact with one another. Faster detectors and read-out electronics are beneficial for both techniques. A higher duty-factor x-ray source allows lower instantaneous count rates at the same overall x-ray intensity, improving the range of applicability of Z-SPEC in particular. Using an intensity-modulated advanced x-ray source (IMAXS) allows reducing the x-ray count rate for cargoes with higher transmission, and a stacked-detector approach may help material discrimination for the lowest attenuations. Image processing and segmentation allow derivation of results for entire objects, and subtraction of backgrounds. We discuss R&D performed under a number of different programs, showing progress made in each of the interacting subsystems. We discuss results of studies into faster scintillation detectors, including ZnO, BaF2 and PbWO4, as well as suitable photo-detectors, read-out and digitization electronics. We discuss high-duty-factor linear

  6. Low cost combined systems for detection of contraband hidden in cargo containers

    International Nuclear Information System (INIS)

    The fruitful cooperation between Egypt and the International Atomic Energy Agency IAEA through the Coordinated Research Project (Contract No.13497/RO) which is concerned with the use of neutron based techniques for the detection of illicit materials and explosives tends to design and construct combined systems to search the contents of cargo containers for illicit trafficking. The systems consist of a scanner based on using γ rays emitted from 60Co source to locate the position of contraband hidden within the payload and an identifier based on using neutrons emitted from Pu-α-Be source to distinguish the suspected object through elemental analysis by fast and thermal neutrons. The systems include as well a especially mechanical system to move the inspected container between the radiation sources and detector. The mechanical system is equipped with an electrical controller unit to adjust the movement increment of the inspected container and time of measurement. A γ ray measuring system with NaI(Tl) detector is used to measure the γ rays transmitted through the container during the scanning process. The same γ detector is used to measure the γ spectrum resulting from neutron interrogation. The measured γ rays are fed to two counting stations, the first station is used to count γ rays transmitted through the container during the scanning process, while the second one is used to record the spectrum of γ ray emitted from the suspected object during the identification stage. Results obtained from the primary tests are presented in the form of 2D image for the suspected object and in the form of displayed γ spectra for γ rays emitted from the suspected objects by neutron interrogation analysis. The constructed 2D images and γ-ray spectra measured for different hidden illicit materials clearly indicate that the system is of low scanning cost, very fast and efficient for detecting contraband screened by steel shield of 1 cm thick. In addition, the system weight

  7. Impact of a radio-frequency identification system and information interchange on clearance processes for cargo at border posts

    Directory of Open Access Journals (Sweden)

    Ernest Bhero

    2015-02-01

    Full Text Available Background: Improved operational efficiency is important to role players in cross-border logistics and trade corridors. Cargo owners and cargo forwarders have been particularly concerned about long delays in the processing and clearing of cargo at border posts. Field studies suggest that these delays are due to a combination of factors, such as a lack of optimum system configurations and non-optimised human-dependent operations, which make the operations prone to corruption and other malpractices.Objectives: This article presents possible strategies for improving some of the operations in this sector. The research hinges on two key questions: (1 what is the impact of information interchange between stakeholders on the cargo transit time and (2 how will cargo transit time be impacted upon by automatic identification of cargo and the status of cargo seals on arriving vehicles at the border?Method: The use of information communication systems enabled by automatic identification systems (incorporating radio-frequency identification technology is suggested.Results: Results obtained by the described simulation model indicate that improvements of up to 82% with regard to transit time are possible using these techniques.Conclusion: The findings therefore demonstrate how operations at border posts can be improved through the use of appropriate technology and configuration of the operations.

  8. The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.

    Science.gov (United States)

    Topalidou, Irini; Cattin-Ortolá, Jérôme; Pappas, Andrea L; Cooper, Kirsten; Merrihew, Gennifer E; MacCoss, Michael J; Ailion, Michael

    2016-05-01

    The dense-core vesicle is a secretory organelle that mediates the regulated release of peptide hormones, growth factors, and biogenic amines. Dense-core vesicles originate from the trans-Golgi of neurons and neuroendocrine cells, but it is unclear how this specialized organelle is formed and acquires its specific cargos. To identify proteins that act in dense-core vesicle biogenesis, we performed a forward genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We previously reported the identification of two conserved proteins that interact with the small GTPase RAB-2 to control normal dense-core vesicle cargo-sorting. Here we identify several additional conserved factors important for dense-core vesicle cargo sorting: the WD40 domain protein EIPR-1 and the endosome-associated recycling protein (EARP) complex. By assaying behavior and the trafficking of dense-core vesicle cargos, we show that mutants that lack EIPR-1 or EARP have defects in dense-core vesicle cargo-sorting similar to those of mutants in the RAB-2 pathway. Genetic epistasis data indicate that RAB-2, EIPR-1 and EARP function in a common pathway. In addition, using a proteomic approach in rat insulinoma cells, we show that EIPR-1 physically interacts with the EARP complex. Our data suggest that EIPR-1 is a new interactor of the EARP complex and that dense-core vesicle cargo sorting depends on the EARP-dependent trafficking of cargo through an endosomal sorting compartment. PMID:27191843

  9. Detection of Special Nuclear Material in Cargo Containers Using Neutron Interrogation

    Energy Technology Data Exchange (ETDEWEB)

    Slaughter, D; Accatino, M; Bernstein, A; Candy, J; Dougan, A; Hall, J; Loshak, A; Manatt, D; Meyer, A; Pohl, B; Prussin, S; Walling, R; Weirup, D

    2003-08-01

    The goal of the work reported here is to develop a concept for an active neutron interrogation system that can detect small targets of SNM contraband in cargo containers, roughly 5 kg HEU or 1 kg Pu, even when well shielded by a thick cargo. It is essential that the concept be reliable and have low false-positive and false-negative error rates. It also must be rapid to avoid interruption of commerce, completing the analysis in minutes. A new radiation signature unique to SNM has been identified that utilizes high-energy (E{sub {gamma}} = 3-7 MeV) fission product {gamma}-ray emission. Fortunately, this high-energy {gamma}-ray signature is robust in that it is very distinct compared to normal background radiation where there is no comparable high-energy {gamma}-ray radiation. Equally important, it has a factor of 10 higher yield than delayed neutrons that are the basis of classical interrogation technique normally used on small unshielded specimens of SNM. And it readily penetrates two meters of low-Z and high-Z cargo at the expected density of {approx} 0.5 gm/cm{sup 3}. Consequently, we expect that in most cases the signature flux at the container wall is at least 2-3 decades more intense than delayed neutron signals used historically and facilitates the detection of SNM even when shielded by thick cargo. Experiments have verified this signature and its predicted characteristics. However, they revealed an important interference due to the activation of {sup 16}O by the {sup 16}O(n,p){sup 16}N reaction that produces a 6 MeV {gamma}-ray following a 7-sec {beta}-decay of the {sup 16}N. This interference is important when irradiating with 14 MeV neutrons but is eliminated when lower energy neutron sources are utilized since the reaction threshold for {sup 16}O(n,p){sup 16}N is 10 MeV. The signature {gamma}-ray fluxes exiting a thick cargo can be detected in large arrays of scintillation detectors to produce useful signal count rates of 2-4 x 10{sup 4} cps. That is high

  10. Detection of Special Nuclear Material in Cargo Containers Using Neutron Interrogation

    International Nuclear Information System (INIS)

    The goal of the work reported here is to develop a concept for an active neutron interrogation system that can detect small targets of SNM contraband in cargo containers, roughly 5 kg HEU or 1 kg Pu, even when well shielded by a thick cargo. It is essential that the concept be reliable and have low false-positive and false-negative error rates. It also must be rapid to avoid interruption of commerce, completing the analysis in minutes. A new radiation signature unique to SNM has been identified that utilizes high-energy (Eγ = 3-7 MeV) fission product γ-ray emission. Fortunately, this high-energy γ-ray signature is robust in that it is very distinct compared to normal background radiation where there is no comparable high-energy γ-ray radiation. Equally important, it has a factor of 10 higher yield than delayed neutrons that are the basis of classical interrogation technique normally used on small unshielded specimens of SNM. And it readily penetrates two meters of low-Z and high-Z cargo at the expected density of ∼ 0.5 gm/cm3. Consequently, we expect that in most cases the signature flux at the container wall is at least 2-3 decades more intense than delayed neutron signals used historically and facilitates the detection of SNM even when shielded by thick cargo. Experiments have verified this signature and its predicted characteristics. However, they revealed an important interference due to the activation of 16O by the 16O(n,p)16N reaction that produces a 6 MeV γ-ray following a 7-sec β-decay of the 16N. This interference is important when irradiating with 14 MeV neutrons but is eliminated when lower energy neutron sources are utilized since the reaction threshold for 16O(n,p)16N is 10 MeV. The signature γ-ray fluxes exiting a thick cargo can be detected in large arrays of scintillation detectors to produce useful signal count rates of 2-4 x 104 cps. That is high enough to quickly identify SNM fission by its characteristic high energy γ-ray emission and

  11. First beam trials of 6 MeV RF electron linac for mobile Cargo-scanning applications

    International Nuclear Information System (INIS)

    The 6 MeV compact RF Electron Linac for container cargo-scanning applications has been designed and developed by the Accelerator and Pulse Power Division, BARC. This compact linac has been designed to be a mobile system, to be mounted in a moving container. In linac-based cargo-scanning systems, the linac acts as the source of X-rays, which fall on the cargo and are then detected by the detector system. This paper describes the salient features of the 6/3 MeV dual energy compact linac and results of the first beam trials. (author)

  12. Design Verification of a High Energy X-ray Scanner for Cargo Inspections with the Stereo Visualization

    International Nuclear Information System (INIS)

    In this study, validity verifications of the design of the system have been carried out with Monte Carlo simulations for the precise technical design. The central goal in this research asks that the designed system is suitable enough for the purpose, which is to inspect cargo container in fast and with good visualization performance. The simulation test of the design of the cargo container inspection system has been carried out using Geant4, which is a widely used Monte Carlo code. The calculation is to expect whether the geometrical design is able to visualize the inside of a cargo with reasonable coverage and the results give an insight to achieve the goal

  13. Photothermal nanoblade for large cargo delivery into mammalian cells

    OpenAIRE

    Wu, Ting-Hsiang; Teslaa, Tara; Kalim, Sheraz; French, Christopher T; Maghadam, Shahriar; Wall, Randolph; Miller, Jeffery F.; Witte, Owen N.; Teitell, Michael A.; Chiou, Pei-Yu

    2011-01-01

    It is difficult to achieve controlled cutting of elastic, mechanically fragile, and rapidly resealing mammalian cell membranes. Here, we report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized explosive vapor bubble, which rapidly punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The cavitation bubble pattern is controlled by the metallic struct...

  14. Status report on the detection of illicit materials in cargo containers by using neutron beams

    International Nuclear Information System (INIS)

    Non-intrusive inspection of cargo containers has become a key issue in the fight against terrorism. Indeed, shipping of cargo container between seaports represent today one of the more important way of the international trade. After 9/11, the Cargo Security Initiative started setting standards for the cargo containers inspections in the trading between US and other countries, as the ones in the European Union (E U). US are now aiming at 100% scanning of cargo containers shipped towards the American ports. At present time, controls are mainly based on X- or gamma-ray scanners, which provide mainly the shape and density of the transported goods. Fast neutrons can be additionally employed to deduce information about their elemental composition. Neutrons have been selected mainly thanks to their capability of penetrating materials and the availability of different type of neutron sources. Neutron transmission measurements or the detection of characteristic gamma-rays emitted following neutron induced reactions have been proposed to characterize carbon, oxygen and nitrogen, which are major components of explosives or narcotics, with the goal of discriminating illicit from benign materials. Several systems have been validated under laboratory conditions demonstrating that such technologies are capable of providing material specific information and few of them have been fielded. However, the use of neutron based tools depends not only on the intrinsic capability of the method, as explored in laboratory conditions, but also on some major issues: 1) the possibility of solving logistics and licensing problems; 2) the possibility of constructing systems that are operated directly by end-users; 3) the effective integration of the neutron based system in current customs procedures. In this talk, the general capability of neutron sources to be used in a cost effective way to detect illicit materials inside cargo containers will be discussed looking also to the latest technical

  15. A review of modeling issues and analysis methods for the thermal response of cargoes transported in the Safe Secure Trailer subjected to fire environments

    International Nuclear Information System (INIS)

    This paper discusses thermal analysis in support of probabilistic risk assessment (PRA) to predict the heating of cargoes shipped in vehicles like the Safe Secure Trailer. Fire environments contribute very significantly to the risk associated with ground transport of special nuclear materials. The tradeoff between thermal model complexity and the affordable number of scenarios used to represent the hazard space is discussed as it impacts PRA. The relevant heat transfer mechanisms are discussed along with the applicability of methods from the literature for analysis of these mechanisms. Many of the subject's real problems remain too complex for affordable and rigorous analysis. Available models are generally restricted to idealizations that are quickly obviated by real effects. Approximate treatment methods, striving to produce conservative, realistic estimates are also discussed

  16. The interplay between RPGR, PDEδ and Arl2/3 regulate the ciliary targeting of farnesylated cargo

    Science.gov (United States)

    Wätzlich, Denise; Vetter, Ingrid; Gotthardt, Katja; Miertzschke, Mandy; Chen, Yong-Xiang; Wittinghofer, Alfred; Ismail, Shehab

    2013-01-01

    Defects in primary cilia result in human diseases known as ciliopathies. The retinitis pigmentosa GTPase regulator (RPGR), mutated in the most severe form of the eye disease, is located at the transition zone of the ciliary organelle. The RPGR-interacting partner PDEδ is involved in trafficking of farnesylated ciliary cargo, but the significance of this interaction is unknown. The crystal structure of the propeller domain of RPGR shows the location of patient mutations and how they perturb the structure. The RPGR·PDEδ complex structure shows PDEδ on a highly conserved surface patch of RPGR. Biochemical experiments and structural considerations show that RPGR can bind with high affinity to cargo-loaded PDEδ and exposes the Arl2/Arl3-binding site on PDEδ. On the basis of these results, we propose a model where RPGR is acting as a scaffold protein recruiting cargo-loaded PDEδ and Arl3 to release lipidated cargo into cilia. PMID:23559067

  17. Radiological risks from irradiation of cargo contents with EURITRACK neutron inspection systems

    Science.gov (United States)

    Giroletti, E.; Bonomi, G.; Donzella, A.; Viesti, G.; Zenoni, A.

    2012-07-01

    The radiological risk for the population related to the neutron irradiation of cargo containers with a tagged neutron inspection system has been studied. Two possible effects on the public health have been assessed: the modification of the nutritional and organoleptic properties of the irradiated materials, in particular foodstuff, and the neutron activation of consumer products (i.e. food and pharmaceuticals). The result of this study is that irradiation of food and foodstuff, pharmaceutical and medical devices in container cargoes would neither modify the properties of the irradiated material nor produce effective doses of concern for public health. Furthermore, the dose received by possible stowaways present inside the container during the inspection is less than the annual effective dose limit defined by European Legislation for the public.

  18. Magnetically triggered release of molecular cargo from iron oxide nanoparticle loaded microcapsules

    Science.gov (United States)

    Carregal-Romero, Susana; Guardia, Pablo; Yu, Xiang; Hartmann, Raimo; Pellegrino, Teresa; Parak, Wolfgang J.

    2014-12-01

    Photothermal release of cargo molecules has been extensively studied for bioapplications. For instance, microcapsules decorated with plasmonic nanoparticles have been widely used in in vitro assays. However, some concerns about their suitability for some in vivo applications cannot be easily overcome, in particular the limited penetration depth of light (even infrared). Magnetic nanoparticles are alternative heat-mediators for local heating, which can be triggered by applying an alternating magnetic field (AMF). AMFs are much less absorbed by tissue than light and thus can penetrate deeper overcoming the above mentioned limitations. Here we present iron oxide nanocube-modified microcapsules as a platform for magnetically triggered molecular release. Layer-by-layer assembled polyelectrolyte microcapsules with 4.6 μm diameter, which had 18 nm diameter iron oxide nanocubes integrated in their walls, were synthesized. The microcapsules were further loaded with an organic fluorescent polymer (Cascade Blue-labelled dextran), which was used as a model of molecular cargo. Through an AMF the magnetic nanoparticles were able to heat their surroundings and destroy the microcapsule walls, leading to a final release of the embedded cargo to the surrounding solution. The cargo release was monitored in solution by measuring the increase in both absorbance and fluorescence signal after the exposure to an AMF. Our results demonstrate that magnetothermal release of the encapsulated material is possible using magnetic nanoparticles with a high heating performance.Photothermal release of cargo molecules has been extensively studied for bioapplications. For instance, microcapsules decorated with plasmonic nanoparticles have been widely used in in vitro assays. However, some concerns about their suitability for some in vivo applications cannot be easily overcome, in particular the limited penetration depth of light (even infrared). Magnetic nanoparticles are alternative heat

  19. Gamma-ray nuclear resonance absorption (γ-NRA) for explosives detection in air cargo

    International Nuclear Information System (INIS)

    The γ-NRA method has been utilized to detect explosives concealed in aviation containers loaded with a variety of cargo. In γ-NRA, gamma-rays at an energy of 9.17 MeV undergo a resonant nuclear attenuation component proportional to the integrated density of 14N nuclei along the line of sight from source to detector. When inspecting objects in transmission mode, projected images of nitrogen density of their contents can be generated. In an experiment performed earlier this year at the Dynamitron accelerator lab. of Birmingham Univ., U.K., diverse items such as passenger bags, electronic equipment, paper goods and mixed cargo were scanned along with explosives simulants. The results from this run will be presented and anticipated performance ratings of an operational explosives detection system (EDS) discussed

  20. Gamma-ray nuclear resonance absorption (γ-NRA) for explosives detection in air cargo

    Science.gov (United States)

    Vartsky, D.; Goldberg, M. B.; Engler, G.; Goldschmidt, A.; Feldman, G.; Bar, D.; Sayag, E.; Katz, D.; Krauss, R. A.

    1999-06-01

    The γ-NRA method has been utilized to detect explosives concealed in aviation containers loaded with a variety of cargo. In γ-NRA, gamma-rays at an energy of 9.17 MeV undergo a resonant nuclear attenuation component proportional to the integrated density of 14N nuclei along the line of sight from source to detector. When inspecting objects in transmission mode, projected images of nitrogen density of their contents can be generated. In an experiment performed earlier this year at the Dynamitron accelerator lab. of Birmingham Univ., U.K., diverse items such as passenger bags, electronic equipment, paper goods and mixed cargo were scanned along with explosives simulants. The results from this run will be presented and anticipated performance ratings of an operational explosives detection system (EDS) discussed.

  1. Shock and vibration environments encountered during normal rail transportation of heavy cargo

    International Nuclear Information System (INIS)

    This study was conducted to obtain vibration and superimposed shock data during normal rail shipment of heavy cargo. The data were obtained during a regularly scheduled rail shipment of a 45-tonne (50-ton) cargo which consisted of an empty spent-fuel container, its supporting structure, and associated hoisting devices. The shipment was made over rail lines which are operated by the Atchison, Topeka, and Santa Fe Railway Company between Denver, Colorado and Albuquerque, New Mexico. The instrumented rail car was equipped with 0.38-m (15-in.) hydraulic end-of-car coupling devices. The 99 percentile levels of vibration acceleration amplitudes and single degree-of-freedom superimposed shock response spectra for the longitudinal, transverse, and vertical axes are presented

  2. Determination of Fire Enviroment in Stacked Cargo Containers with Radioactive Materials Packages

    Energy Technology Data Exchange (ETDEWEB)

    Arviso, M.; Bobbe, J.G.; Dukart, R.D.; Koski, J.A.

    1999-05-01

    Results from a Fire Test with a three-by-three stack of standard 6 m long International Standards Organization shipping containers containing combustible fuels and empty radioactive materials packages are reported and discussed. The stack is intended to simulate fire conditions that could occur during on-deck stowage on container cargo ships. The fire is initated by locating the container stack adjacent to a 9.8 x 6 m pool fire. Temperatures of both cargoes (empty and simulated radioactive materials packages) and containers are recorded and reported. Observations on the duration, intensity and spread of the fire are discussed. Based on the results, models for simulation of fire exposure of radioactive materials packages in such fires are suggested.

  3. An airport cargo inspection system based on X-ray and thermal neutron analysis (TNA)

    International Nuclear Information System (INIS)

    A cargo inspection system incorporating a high-resolution X-ray imaging system with a material-specific detection system based on Ancore Corporation's patented thermal neutron analysis (TNA) technology can detect bulk quantities of explosives and drugs concealed in trucks or cargo containers. The TNA process utilises a 252Cf neutron source surrounded by a moderator. The neutron interactions with the inspected object result in strong and unique gamma-ray signals from nitrogen, which is a key ingredient in modern high explosives, and from chlorinated drugs. The TNA computer analyses the gamma-ray signals and automatically determines the presence of explosives or drugs. The radiation source terms and shielding design of the facility are described. For the X-ray generator, the primary beam, leakage radiation, and scattered primary and leakage radiation were considered. For the TNA, the primary neutrons and tunnel scattered neutrons as well as the neutron-capture gamma rays were considered. (authors)

  4. The insurance of bulk oil cargoes and adjustment of shortage claims

    International Nuclear Information System (INIS)

    Shortage claims discussed in this article include those due to a definite disaster such as a fire, those due to cargo contamination and storage, and those identified in documentation as occurring between loading and unloading. The principal types of cover are examined, and the distinction between gross and net quantities, claim documentation, the petroleum measurement tables, and guaranteed out turn cover are described in detail. (UK)

  5. Automated Workplace of Cargo Consignor: New : Development of the Promotion Strategy for a Software Product

    OpenAIRE

    Soltysyuk, Anastasiya

    2012-01-01

    The aim of the thesis was to develop a promotion strategy of a new software product: “Automated Workplace of Cargo Consignor: New” for the commissioner company “TMSoft ltd”. Main method used in this case was table research and information collection; marketing literature was carefully studied, as well as legislative sources. Theoretical part consists of the situational analysis, strategy pyramid and promotion theory. And the development part is the core of the thesis, and includes the pro...

  6. RESEARCH ACTION: IMPLEMENTATION ZERO BASED BUDGET (ZBB) IN THE PROVIDER SERVICE LEASING EQUIPAMENTS OF CARGO HANDLING.

    OpenAIRE

    Levi Gimenez; Antonio Benedito Silva Oliveira

    2013-01-01

    This article aims to present the implementation of zero-based budgeting in a rental service provider of cargo handling equipment using the action research methodology. The goal was to examine the possibility of concomitant use of this instrument in service providers in need of accurate information that enables targeting at the best result in a setting avid for quick decisions and actions. Action research was used as research method. It was concluded that it is suitable for this branch, con...

  7. Ports sustainability: a life cycle assessment of zero emission cargo handling equipment:

    OpenAIRE

    Jerman, Boris; Vujičić, Andrija; Zrnić, Nenad Đ.

    2013-01-01

    The goal of this paper is to present and evaluate the latest trends in the cargo handling equipment (CHE) industry, aimed at mitigating the environmental impact of container terminal operations and contributing to the sustainability of ports. The most common machines for handling containers are described and dealt with separately, with a focus on electric CHE, usually referred to as zero emission CHE. In a separate chapter, recommendations on methodologies suitable for investigation of the en...

  8. IMPACT OF INFORMATION ON THE DELIVERY OF CARGO WITH AIR TRANSPORT

    OpenAIRE

    Габріелова, Т.Ю.; National Aviation University; Войцеховський, В.С.; National Aviation University

    2014-01-01

    Peculiarities of the impact of informatization on the process of cargo delivery involving air transport under conditions of competitive environment dynamic development have been considered. Information products providing uploading capacity on an extensive air route network were analysed. The role of international mediation bodies for financial settlements between participants of air transportation process was determined. Basic aspects of the pricing control depending on the condition of fligh...

  9. STRUCTURAL CHANGES IN PORT CARGO FLOW DISTRIBUTION IN ASIAN CONTAINER PORT SYSTEMS

    OpenAIRE

    Itoh, Hidekazu

    2012-01-01

    International audience This study discusses the structural changes in port cargo flow distribution in Asia and traffic inequality in Asian container port systems, by conducting an inequality decomposition analysis of the Gini index. Specifically, port systems considered here comprise 229 container ports, which were classified into fifteen port ranges according to their geographic locations and annual port container traffic data from 1980 to 2009. The results confirmed the trend of shrinkin...

  10. High Power Nuclear Electric Propulsion (NEP) for Cargo and Propellant Transfer Missions in Cislunar Space

    Science.gov (United States)

    Falck, Robert D.; Borowski, Stanley K.

    2003-01-01

    The performance of Nuclear Electric Propulsion (NEP) in transporting cargo and propellant from Low Earth Orbit (LEO) to the first Earth-Moon Lagrange point (EML1) is examined. The baseline NEP vehicle utilizes a fission reactor system with Brayton power conversion for electric power generation to power multiple liquid hydrogen magnetoplasmadynamic (MPD) thrusters. Vehicle characteristics and performance levels are based on technology availability in a fifteen to twenty year timeframe. Results of numerical trajectory analyses are also provided.

  11. AMPA Receptors Commandeer an Ancient Cargo Exporter for Use as an Auxiliary Subunit for Signaling

    OpenAIRE

    Nadine Harmel; Barbara Cokic; Gerd Zolles; Henrike Berkefeld; Veronika Mauric; Bernd Fakler; Valentin Stein; Nikolaj Klöcker

    2012-01-01

    Fast excitatory neurotransmission in the mammalian central nervous system is mainly mediated by ionotropic glutamate receptors of the AMPA subtype (AMPARs). AMPARs are protein complexes of the pore-lining alpha-subunits GluA1-4 and auxiliary beta-subunits modulating their trafficking and gating. By a proteomic approach, two homologues of the cargo exporter cornichon, CNIH-2 and CNIH-3, have recently been identified as constituents of native AMPARs in mammalian brain. In heterologous reconstit...

  12. RAPID ACQUISITION OF ENVIRONMENTAL INFORMATION AFTER ACCIDENTS WITH HAZARDOUS CARGO THROUGH REMOTE SENSING BY UAV

    OpenAIRE

    Longhitano, G. A.; Quintanilh, J. A.

    2013-01-01

    The aim of the study was to present procedures of using remote sensing by UAV to aid in the rapid evaluation and monitoring of environmental impacts caused by accidents involving transport of dangerous cargoes on highways. From the data collected and the UAV model defined, it was developed a methodology of operating a UAV to generate the potential benefits identified in the application. A Mini UAV or Close Range class was considered appropriate. It was created the procedures for imag...

  13. Technologies and technical tools of combined transportation of cargoes by factors of global logistics

    Directory of Open Access Journals (Sweden)

    Анатолій Миколайович Котенко

    2015-01-01

    Full Text Available The new piggybacks technology has been developed and presented taking into account the problems of global logistics. The technology allows cargo operations (loading and unloading of modules on platforms of varying floor height above rail head level and reload modules between platforms with different values and different ways between rails floor height platforms. The system of differential equations of Kolmogorov have been showed and modeling of combined transportation have been made

  14. Evaluating the modal split of overland transportation of general cargo in Brazil using a market share model

    Directory of Open Access Journals (Sweden)

    Francisco Gildemir Ferreira da Silva

    2014-10-01

    Full Text Available This paper presents a methodology developed to evaluate the modal split of land transport of general cargo in Brazil and possible reasons for the prevalence of road transport over rail, using a market share model. No studies were found using the market share model for planning cargo transport, in spite of its generic nature which makes it readily applicable to any market, service, utility or commodity. The methodology was applied in three transport corridors, two groups of general cargo and enabled us to establish that Brazilian rail operations are still uncompetitive in terms of logistics costs when transporting general cargo of high aggregate value. Moreover, transport supply is a determinant factor for the greater use of road transport in Brazil’s general cargo transport matrix, and the demand for general cargo transport in Brazil is elastic in relation to this factor. For low aggregate value general cargo (AV1, a 1% reduction in the gap between road and railway supply leads to an increase in the railway market share of 4.5% through TC1 (São Paulo - Porto Alegre - São Paulo, 4.9% through TC2 (Santos - Brasilia - Santos, and 3.3% through TC3(São Paulo - Rio de Janeiro - São Paulo. For high aggregate value general cargo (AV2, this elasticity was more pronounced. A 1% reduction in the gap between road and railway transport supply leads to an increase in the railway market share of 4.2% through TC1, 11.9% through TC2, and 9.6% through TC3.

  15. 46 CFR 32.60-10 - Segregation of cargo; Grade A, B, C, or D-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Segregation of cargo; Grade A, B, C, or D-TB/ALL. 32.60-10 Section 32.60-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL... 1, 1951 § 32.60-10 Segregation of cargo; Grade A, B, C, or D—TB/ALL. (a) General. The...

  16. Sensitivity of a Multi-view Bremsstrahlung Scanning Technique for Radiography of Intermodal Cargo Containers to Identify Fisssionable Materials

    OpenAIRE

    Hansen, Araina

    2014-01-01

    Rapid scanning of intermodal cargo containers for clandestine fissionable material is a security concern of the United States government. This issue has been investigated over the past ten years with limited success. This dissertation builds on previous simulation work to investigate the limits of a multi-view scanning technique to determine the linear attenuation coefficients of small high-Z objects hidden within cargo containers. Monte Carlo modeling was used to estimate the uncertainties i...

  17. Temperature Controlled Aircraft Unit Load Devices: The Technological Response to Growing Global Air Cargo Cool Chain Requirements

    OpenAIRE

    Glenn Baxter; Kyriakos Kourousis

    2015-01-01

    Unit load devices (ULDs) are pallets and containers which are used to carry air cargo, mail and passengers baggage on wide-body aircraft. Using an in-depth case study, an empirical investigation was undertaken to examine the recent technological developments and innovations in the temperature-controlled containers that are used extensively in the growing and highly important air cargo cool chains. The results demonstrated that advances in refrigeration systems and the use of composite materia...

  18. Low cost, robust and real time system for detecting and tracking moving objects to automate cargo handling in port terminals

    OpenAIRE

    Vaquero, Victor; Repiso, Ely; Sanfeliu, Alberto; Vissers, John; Kwakkernaat, Maurice

    2015-01-01

    The presented paper addresses the problem of detecting and tracking moving objects for autonomous cargo handling in port terminals using a perception system which input data is a single layer laser scanner. A computationally low cost and robust Detection and Tracking Moving Objects (DATMO) algorithm is presented to be used in autonomous guided vehicles and autonomous trucks for efficient transportation of cargo in ports. The method first detects moving objects and then tracks them, taking int...

  19. Amphiphilic nanocapsules entangled with organometallic coordination polymers for controlled cargo release.

    Science.gov (United States)

    Liang, Guodong; Ni, Huan; Bao, Suping; Zhu, Fangming; Gao, Haiyang; Wu, Qing; Tang, Ben Zhong

    2014-06-01

    A class of new amphiphilic nanocapsules entangled with organometallic coordination polymers has been developed for the first time. Poly(2-(N,N-dimethyl amino)ethyl methacrylate)-b-polystyrene capped with β-cyclodextrin (β-CD) (CD-PDMAEMA-b-PS) is first synthesized using sequent RAFT polymerization of styrene and 2-(N,N-dimethyl amino)ethyl methacrylate with xanthate modified β-CD as chain transfer agent. The end group of β-CD is allowed to include 4,4'-bipyridine through host-guest inclusion to yield PDMAEMA-b-PS terminated with an inclusion complex of β-CD and bipyridine (bpy-PDMAEMA-b-PS), which is then used as surfactant to prepare emulsion droplets in toluene/water mixture. Upon addition of Ni(II), bipyridine coordinates with Ni(II) to form coordination polymers in the periphery of emulsion droplets, affording amphiphilic capsules entangled with organometallic coordination polymers, as confirmed by GPC, (1)H NMR, SEM, TEM, DLS, and so on. The organometallic coordination polymer capsules are capable of encapsulating organic cargoes. Interestingly, encapsulated cargoes can be extracted from the capsules without damaging the capsules. Such capsules are potential candidates for encapsulating and controlled release of organic cargoes. PMID:24828951

  20. DISEÑO ESTRATÉGICO DE PERFILES DE CARGOS POR COMPETENCIAS.

    Directory of Open Access Journals (Sweden)

    Ariel Soltura Laseria

    2008-01-01

    Full Text Available En el trabajo se presenta una tecnología para la determinación de los perfiles de cargo por competencias en las organizaciones cubanas con base científica, fundamentada en la creciente necesidad de dirigir los esfuerzos individuales hacia el cumplimiento de la estrategia. La tecnología provee a las organizaciones de una forma de determinar la estructura de competencias que ha de adoptar para satisfacer las demandas o necesidades de clientes o usuarios, estado, proveedores y sociedad, en función de su cultura, procesos, estructura, rivalidad de competidores -en caso que exista y la estrategia como guía del proceso, donde los perfiles de cargo por competencias desempeñan el papel fundamental para la implantación de la estrategia. Palabras Clave Gestión de (por competencias, perfiles de competencias, diseño estratégico de perfiles de cargo por competencias.

  1. Reliability analysis of the bulk cargo loading system including dependent components

    Science.gov (United States)

    Blokus-Roszkowska, Agnieszka

    2016-06-01

    In the paper an innovative approach to the reliability analysis of multistate series-parallel systems assuming their components' dependency is presented. The reliability function of a multistate series system with components dependent according to the local load sharing rule is determined. Linking these results for series systems with results for parallel systems with independent components, we obtain the reliability function of a multistate series-parallel system assuming dependence of components' departures from the reliability states subsets in series subsystem and assuming independence between these subsystems. As a particular case, the reliability function of a multistate series-parallel system composed of dependent components having exponential reliability functions is fixed. Theoretical results are applied practically to the reliability evaluation of a bulk cargo transportation system, which main area is to load bulk cargo on board the ships. The reliability function and other reliability characteristics of the loading system are determined in case its components have exponential reliability functions with interdependent departures rates from the subsets of their reliability states. Finally, the obtained results are compared with results for the bulk cargo transportation system composed of independent components.

  2. Determination of ambient and personal dose equivalent for personnel and cargo security screening

    International Nuclear Information System (INIS)

    In the past few years, imaging technology using ionising radiation has been gaining in importance for the screening of goods and persons for security reasons and in order to detect contraband. For radiation protection purposes it is extremely important to know that dose persons are exposed to when passing through a personnel scanner or, as a stowaway, in a cargo scanner, so as to remain within the prescribed dose limits. Within the scope of a research project, measurements were performed on different types of personnel X-ray scanners as well as cargo X-ray scanners, using the transmission and/or the backscattering method. All scanners investigated operate with a high dose rate and use short irradiation time. Owing to this method of scanning reliable values can only be determined for the personal and ambient dose equivalents, Hp(10) and H*(10), by using a specially developed measuring system. The aim of this project was to determine the range of magnitudes of doses for representative personnel and cargo X-ray scanner systems. Depending on the type of scanner, the determined dose values for personnel scanners range from 0.07 μSv to 6 μSv. Measurements and instruments used in this study are described and the dose values obtained are discussed in detail. (authors)

  3. Video-based cargo fire verification system with fuzzy inference engine for commercial aircraft

    Science.gov (United States)

    Sadok, Mokhtar; Zakrzewski, Radek; Zeliff, Bob

    2005-02-01

    Conventional smoke detection systems currently installed onboard aircraft are often subject to high rates of false alarms. Under current procedures, whenever an alarm is issued the pilot is obliged to release fire extinguishers and to divert to the nearest airport. Aircraft diversions are costly and dangerous in some situations. A reliable detection system that minimizes false-alarm rate and allows continuous monitoring of cargo compartments is highly desirable. A video-based system has been recently developed by Goodrich Corporation to address this problem. The Cargo Fire Verification System (CFVS) is a multi camera system designed to provide live stream video to the cockpit crew and to perform hotspot, fire, and smoke detection in aircraft cargo bays. In addition to video frames, the CFVS uses other sensor readings to discriminate between genuine events such as fire or smoke and nuisance alarms such as fog or dust. A Mamdani-type fuzzy inference engine is developed to provide approximate reasoning for decision making. In one implementation, Gaussian membership functions for frame intensity-based features, relative humidity, and temperature are constructed using experimental data to form the system inference engine. The CFVS performed better than conventional aircraft smoke detectors in all standardized tests.

  4. Early results utilizing high-energy fission product gamma rays to detect fissionable material in cargo

    International Nuclear Information System (INIS)

    Full text: A concept for detecting the presence of special nuclear material (235U or 239Pu) concealed in inter modal cargo containers is described. It is based on interrogation with a pulsed beam of 6-8 MeV neutrons and fission events are identified between beam pulses by their β-delayed neutron emission or β -delayed high-energy γ-radiation. The high-energy γ-ray signature is being employed for the first time. Fission product γ-rays above 3 MeV are distinct from natural radioactivity and from nearly all of the induced activity in a normal cargo. High-energy γ-radiation is nearly 10X more abundant than the delayed neutrons and penetrates even thick cargo's readily. The concept employs two large (8x20 ft) arrays of liquid scintillation detectors that have high efficiency for the detection of both delayed neutrons and delayed γ-radiation. Detector backgrounds and potential interferences with the fission signature radiation have been identified and quantified. This information, together with predicted signature strength, has been applied to the estimation of detection probability for the nuclear material and estimation of false alarm rates. This work was performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48

  5. The South America East Coast Reefer Cargo: A Diagnosis of a Competitive Market

    Directory of Open Access Journals (Sweden)

    Galvão Cassia Bömer

    2014-12-01

    Full Text Available This paper analyses reefer cargo maritime shipping characteristics and development in South American East Coast ports. The geographical area studied goes from Punta Arenas (Chile to Manaus (Brazil. The study main hypothesis is reefer shipping as a profitable market niche in spite of higher operation costs and a very demanding supply chain. The paper comprises three sections: the first one describes global reefer cargo transportation general characteristics; the second, reefer shipping in South American ports, focusing five main issues: (1 Cargo flows; (2 Ports infrastructure; (3 Post-Panamax vessels operation and its economies of scale; (4 Shippers and carriers’ mergers and acquisitions processes and (5 Containerization process; finally, it investigates the marketing relationship between shippers and shipping liners. The exploratory study was based on technical reports and statistics analysis, and interviews with industry executives, performing a multicase study. The general conclusions indicate that South American reefer market has concluded most of its containerization process and is moving fast towards a concentrated market of players. Some experts consider that the market will keep increasing as a result of the improved quality of products and enhancing cold logistics services provision in spite of the international crisis, due to emergent markets buying and more demanding clients.

  6. Dose to drivers during drive-through cargo scanning using GEANT4 Monte Carlo simulation

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, Rogerio S.; Gomes, Joana D' Arc R.L.; Costa, Mara Lucia L., E-mail: rogeriog@cnen.gov.br, E-mail: jlopes@cnen.gov.br, E-mail: mara@cnen.gov.br [Comissao Nacional de Energia Nuclear (CNEN-RJ), Rio de Janeiro, RJ (Brazil). Diretoria de Radioprotecao e Seguranca muclear; Miranda, Valeria F.E.S., E-mail: mvaleria@cnen.gov.br [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2013-07-01

    The use of radiation technologies to perform screening for cargo containers has been increased due to security issues, mainly, as a consequence of the United States (US) legislation which requires, from 2013, the scanning of all intermodal cargo containers which arrive at US ports. Currently, systems to cargo inspections, using accelerator-driven high energy X-rays, between 4 and 9 MeV, are available for scanning operations. It is expected that, in the future, the use of these systems will be widely spread on roads, ports and airports in Brazil. However, in order to improve the productivity and reduce the costs of acquisition, operation and maintenance these systems require that the driver drives its vehicle through irradiation area, in a situation where members of the public (the truck drivers) enter in controlled area and are deliberately exposed to high-energy beam. Some manufacturers justifies this procedure arguing that the drivers are exposed briefly, and only to the scattered beam, since there are safety systems in order to avoid that the drivers are exposed to direct beam. In this work, it is presented the preliminary results of Monte Carlo simulations concerning the dose of drivers during scanning operations, including the dose due to a failure of safety system, producing an exposure of drivers to the direct beam, as well as, an analysis of the justification of practice, mainly related to the drive-through operational procedure. (author)

  7. Challenges and perspectives of transport cargo vehicles utilization for performing research in free flight

    Science.gov (United States)

    Matveeva, T. V.; Belyaev, M. Yu.; Tsvetkov, V. V.

    2014-01-01

    Russian Progress transport cargo vehicles have successfully been used in different space station programs since 1978. At present time, they play an important role in the International Space Station (ISS) project. Main tasks performed by the transport cargo vehicle (TCV) in the station program are the following: refueling of the station, delivery of consumables and equipment, waste removal, station attitude control and orbit correction maneuver execution. At the same time, the cargo vehicle basic systems still retain unused resources after the vehicle finishes its work with the station. It makes sense to use these resources to perform research in free flight of TCV after departure from the ISS when possible. The fields of research can be determined not only on the basis of the vehicle capabilities as a research platform but also taking into account needs of the research community. Possible fields could be the following: Earth or other objects remote sensing using additional equipment, Microgravity research aboard TCV, Launch of small satellites and probes after TCV undocking from the station and transfer to the specified orbit, etc. Solution of research tasks using the Progress TCV resources helps to increase efficiency of the ISS research program performance. The paper considers the TCV flight control features and the methods of the solution of the problems arising when various experiments are performed aboard the vehicle.

  8. The nuclear car wash: A system to detect nuclear weapons in commercial cargo shipments

    International Nuclear Information System (INIS)

    A concept for detecting the presence of special nuclear material (235U or 239Pu) concealed in intermodal cargo containers has been developed, studied, and recent performance results are described. It is based on interrogation with a pulsed beam of 3-7 MeV neutrons that produce fission events and subsequent detection of their β-delayed neutron emission or β-delayed high-energy γ-radiation reveals the presence of fissionable material. Fission product β-delayed γ-rays above 3 MeV are nearly 10 times more abundant than β-delayed neutrons and are distinct from natural radioactivity and from nearly all of the induced activity in a normal cargo. Detector backgrounds and potential interferences with the fission signature radiation have been identified and quantified. Their impact on detection sensitivity is relatively minor and can be addressed readily. Components of a simple laboratory prototype have been assembled, tested with the most challenging cargo threat scenarios, and results compared to computer simulations. Preliminary results will be presented

  9. AMPA receptors commandeer an ancient cargo exporter for use as an auxiliary subunit for signaling.

    Directory of Open Access Journals (Sweden)

    Nadine Harmel

    Full Text Available Fast excitatory neurotransmission in the mammalian central nervous system is mainly mediated by ionotropic glutamate receptors of the AMPA subtype (AMPARs. AMPARs are protein complexes of the pore-lining α-subunits GluA1-4 and auxiliary β-subunits modulating their trafficking and gating. By a proteomic approach, two homologues of the cargo exporter cornichon, CNIH-2 and CNIH-3, have recently been identified as constituents of native AMPARs in mammalian brain. In heterologous reconstitution experiments, CNIH-2 promotes surface expression of GluAs and modulates their biophysical properties. However, its relevance in native AMPAR physiology remains controversial. Here, we have studied the role of CNIH-2 in GluA processing both in heterologous cells and primary rat neurons. Our data demonstrate that CNIH-2 serves an evolutionarily conserved role as a cargo exporter from the endoplasmic reticulum (ER. CNIH-2 cycles continuously between ER and Golgi complex to pick up cargo protein in the ER and then to mediate its preferential export in a coat protein complex (COP II dependent manner. Interaction with GluA subunits breaks with this ancestral role of CNIH-2 confined to the early secretory pathway. While still taking advantage of being exported preferentially from the ER, GluAs recruit CNIH-2 to the cell surface. Thus, mammalian AMPARs commandeer CNIH-2 for use as a bona fide auxiliary subunit that is able to modify receptor signaling.

  10. System/subsystem specifications for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB)

    Energy Technology Data Exchange (ETDEWEB)

    Rollow, J.P.; Shipe, P.C.; Truett, L.F. [Oak Ridge National Lab., TN (United States); Faby, E.Z.; Fluker, J.; Grubb, J.; Hancock, B.R. [Univ. of Tennessee, Knoxville, TN (United States); Ferguson, R.A. [Science Applications International Corp., Oak Ridge, TN (United States)

    1995-11-20

    A system is being developed by the Military Traffic Management Command (MTMC) to provide data integration and worldwide management and tracking of surface cargo movements. The Integrated Cargo Database (ICDB) will be a data repository for the WPS terminal-level system, will be a primary source of queries and cargo traffic reports, will receive data from and provide data to other MTMC and non-MTMC systems, will provide capabilities for processing Advance Transportation Control and Movement Documents (ATCMDs), and will process and distribute manifests. This System/Subsystem Specifications for the Worldwide Port System Regional ICDB documents the system/subsystem functions, provides details of the system/subsystem analysis in order to provide a communication link between developers and operational personnel, and identifies interfaces with other systems and subsystems. It must be noted that this report is being produced near the end of the initial development phase of ICDB, while formal software testing is being done. Following the initial implementation of the ICDB system, maintenance contractors will be in charge of making changes and enhancing software modules. Formal testing and user reviews may indicate the need for additional software units or changes to existing ones. This report describes the software units that are components of this ICDB system as of August 1995.

  11. Cargo identification algorithms facilitating unmanned/unattended inspection at high throughput portals

    Science.gov (United States)

    Chalmers, Alex

    2007-10-01

    A simple model is presented of a possible inspection regimen applied to each leg of a cargo containers' journey between its point of origin and destination. Several candidate modalities are proposed to be used at multiple remote locations to act as a pre-screen inspection as the target approaches a perimeter and as the primary inspection modality at the portal. Information from multiple data sets are fused to optimize the costs and performance of a network of such inspection systems. A series of image processing algorithms are presented that automatically process X-ray images of containerized cargo. The goal of this processing is to locate the container in a real time stream of traffic traversing a portal without impeding the flow of commerce. Such processing may facilitate the inclusion of unmanned/unattended inspection systems in such a network. Several samples of the processing applied to data collected from deployed systems are included. Simulated data from a notional cargo inspection system with multiple sensor modalities and advanced data fusion algorithms are also included to show the potential increased detection and throughput performance of such a configuration.

  12. Radiological risks from irradiation of cargo contents with EURITRACK neutron inspection systems

    International Nuclear Information System (INIS)

    The radiological risk for the population related to the neutron irradiation of cargo containers with a tagged neutron inspection system has been studied. Two possible effects on the public health have been assessed: the modification of the nutritional and organoleptic properties of the irradiated materials, in particular foodstuff, and the neutron activation of consumer products (i.e. food and pharmaceuticals). The result of this study is that irradiation of food and foodstuff, pharmaceutical and medical devices in container cargoes would neither modify the properties of the irradiated material nor produce effective doses of concern for public health. Furthermore, the dose received by possible stowaways present inside the container during the inspection is less than the annual effective dose limit defined by European Legislation for the public. - Highlights: ► Neutron irradiation of cargo containers implies a radiological risk. ► The risk is about the modification of food properties and the products activation. ► Assessment is made about the EURITRACK neutron irradiation system. ► Results show that the EURITRACK scanning is not dangerous for the population.

  13. Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein.

    Science.gov (United States)

    Liu, Yang; Salter, Hannah K; Holding, Andrew N; Johnson, Christopher M; Stephens, Elaine; Lukavsky, Peter J; Walshaw, John; Bullock, Simon L

    2013-06-01

    Cytoplasmic dynein is the major minus end-directed microtubule motor in eukaryotes. However, there is little structural insight into how different cargos are recognized and linked to the motor complex. Here we describe the 2.2 Å resolution crystal structure of a cargo-binding region of the dynein adaptor Bicaudal-D (BicD), which reveals a parallel coiled-coil homodimer. We identify a shared binding site for two cargo-associated proteins-Rab6 and the RNA-binding protein Egalitarian (Egl)-within a region of the BicD structure with classical, homotypic core packing. Structure-based mutagenesis in Drosophila provides evidence that occupancy of this site drives association of BicD with dynein, thereby coupling motor recruitment to cargo availability. The structure also contains a region in which, remarkably, the same residues in the polypeptide sequence have different heptad registry in each chain. In vitro and in vivo analysis of a classical Drosophila dominant mutation reveals that this heterotypic region regulates the recruitment of dynein to BicD. Our results support a model in which the heterotypic segment is part of a molecular switch that promotes release of BicD autoinhibition following cargo binding to the neighboring, homotypic coiled-coil region. Overall, our data reveal a pivotal role of a highly asymmetric coiled-coil domain in coordinating the assembly of cargo-motor complexes. PMID:23723415

  14. Fuel Economy and Emissions Effects of Low Tire Pressure, Open Windows, Roof Top and Hitch-Mounted Cargo, and Trailer

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, John F [ORNL; Huff, Shean P [ORNL; West, Brian H [ORNL

    2014-01-01

    To quantify the fuel economy (FE) effect of some common vehicle accessories or alterations, a compact passenger sedan and a sport utility vehicle (SUV) were subjected to SAE J2263 coastdown procedures. Coastdowns were conducted with low tire pressure, all windows open, with a roof top or hitch-mounted cargo carrier, and with the SUV pulling an enclosed cargo trailer. From these coastdowns, vehicle dynamometer coefficients were developed which enabled the execution of vehicle dynamometer experiments to determine the effect of these changes on vehicle FE and emissions over standard drive cycles and at steady highway speeds. The FE penalty associated with the rooftop cargo box mounted on the compact sedan was as high as 25-27% at higher speeds, where the aerodynamic drag is most pronounced. For both vehicles, use of a hitch mounted cargo tray carrying a similar load resulted in very small FE penalties, unlike the rooftop cargo box. The results for the SUV pulling a 3500 pound enclosed cargo trailer were rather dramatic, resulting in FE penalties ranging from 30%, for the city cycle, to 50% at 80 mph, at which point significant CO generation indicated protective enrichment due to high load. Low tire pressure cases resulted in negligible to 10% FE penalty depending on the specific case and test point. Driving with all four windows open decreased FE by 4-8.5% for the compact sedan, and 1-4% for the SUV.

  15. Lead Tungstate and Silicon Photomultipliers for Transmission Z-spectroscopy in Cargo Inspection Systems

    Science.gov (United States)

    Langeveld, Willem G. J.; Janecek, Martin

    The bremsstrahlung X-ray spectrum in high-energy, high-intensity X-ray cargo inspection systems is attenuated and modified by cargo materials depending on the cargo atomic number (Z). Spectroscopy of transmitted x rays is thus useful to measure the approximate Z of the cargo. Due to the broad features of the energy spectrum, excellent energy resolution is not required. Such "Z-Spectroscopy" (Z-SPEC) is possible at low enough count rates. A statistical approach, Z-SCAN (Z-determination by Statistical Count-rate ANalysis), can also be used, complementing Z-SPEC at high count rates. Both approaches require fast X-ray detectors and fast digitizers. Z-SPEC, in particular, benefits from very fast scintillators, in order to avoid signal pile-up.Preferentially, Z-SPEC, Z-SCAN and cargo imaging are implemented in a single detector array to reduce system cost, weight, and complexity. To preserve good spatial resolution of the imaging subsystem, dense scintillators are required. Previously, we studied ZnO, BaF2 and PbWO4, as well as suitable photo-detectors, read-out electronics and digitizers. ZnO is not suitable because it self-absorbs its scintillation light. BaF2 emits in the UV, either requiring fast wavelength shifters or UV-sensitive solid state read-out devices, and it also has a long decay time component. PbWO4 is currently the most attractive choice because it does not have these problems, but it is significantly slower and has low light output. There is thus a need for alternative fast high-density scintillators that emit visible light. Alternatively, there is a need for a fast solid-state read-out device that is sensitive to UV light for use with BaF2, or other UV-emitting scintillators. Here, we present results of tests performed with PbWO4 crystals, reflector materials and silicon photomultipliers.

  16. The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.

    Directory of Open Access Journals (Sweden)

    Irini Topalidou

    2016-05-01

    Full Text Available The dense-core vesicle is a secretory organelle that mediates the regulated release of peptide hormones, growth factors, and biogenic amines. Dense-core vesicles originate from the trans-Golgi of neurons and neuroendocrine cells, but it is unclear how this specialized organelle is formed and acquires its specific cargos. To identify proteins that act in dense-core vesicle biogenesis, we performed a forward genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We previously reported the identification of two conserved proteins that interact with the small GTPase RAB-2 to control normal dense-core vesicle cargo-sorting. Here we identify several additional conserved factors important for dense-core vesicle cargo sorting: the WD40 domain protein EIPR-1 and the endosome-associated recycling protein (EARP complex. By assaying behavior and the trafficking of dense-core vesicle cargos, we show that mutants that lack EIPR-1 or EARP have defects in dense-core vesicle cargo-sorting similar to those of mutants in the RAB-2 pathway. Genetic epistasis data indicate that RAB-2, EIPR-1 and EARP function in a common pathway. In addition, using a proteomic approach in rat insulinoma cells, we show that EIPR-1 physically interacts with the EARP complex. Our data suggest that EIPR-1 is a new interactor of the EARP complex and that dense-core vesicle cargo sorting depends on the EARP-dependent trafficking of cargo through an endosomal sorting compartment.

  17. Evaluation of a flag-based explosives detection algorithm based on active neutron interrogation for use in sea land cargo containers

    International Nuclear Information System (INIS)

    Highly explosive materials smuggled in sea land cargo containers are a security concern. An algorithm based on neutron and photon measurements during active neutron interrogation with 14.1 MeV neutrons was developed. The detection algorithm has now been evaluated for effectiveness over a wide range of explosive sizes, positions, and cargo configurations. Various sources of uncertainty were also studied. An estimate of the false positive and false negative rates was completed for various measurement conditions. Results showed that, although minimum detectable mass depends on the surrounding cargo, explosive position, and cargo configuration, 200 kg RDX could be reliably detected. (author)

  18. Transport properties of melanosomes along microtubules interpreted by a tug-of-war model with loose mechanical coupling.

    Directory of Open Access Journals (Sweden)

    Sebastián Bouzat

    Full Text Available In this work, we explored theoretically the transport of organelles driven along microtubules by molecular motors of opposed polarities using a stochastic model that considers a Langevin dynamics for the cargo, independent cargo-motor linkers and stepping motion for the motors. It has been recently proposed that the stiffness of the motor plays an important role when multiple motors collectively transport a cargo. Therefore, we considered in our model the recently reported values for the stiffness of the cargo-motor linker determined in living cells (∼0.01 pN/nm, which is significantly lower than the motor stiffness obtained in in vitro assays and used in previous studies. Our model could reproduce the multimodal velocity distributions and typical trajectory characteristics including the properties of the reversions in the overall direction of motion observed during melanosome transport along microtubules in Xenopus laevis melanophores. Moreover, we explored the contribution of the different motility states of the cargo-motor system to the different modes of the velocity distributions and could identify the microscopic mechanisms of transport leading to trajectories compatible with those observed in living cells. Finally, by changing the attachment and detachment rates, the model could reproduce the different velocity distributions observed during melanosome transport along microtubules in Xenopus laevis melanophores stimulated for aggregation and dispersion. Our analysis suggests that active tug-of-war processes with loose mechanical coupling can account for several aspects of cargo transport along microtubules in living cells.

  19. Opposing microtubule motors control motility, morphology and cargo segregation during ER-to-Golgi transport

    Directory of Open Access Journals (Sweden)

    Anna K. Brown

    2014-04-01

    Full Text Available We recently demonstrated that dynein and kinesin motors drive multiple aspects of endosomal function in mammalian cells. These functions include driving motility, maintaining morphology (notably through providing longitudinal tension to support vesicle fission, and driving cargo sorting. Microtubule motors drive bidirectional motility during traffic between the endoplasmic reticulum (ER and Golgi. Here, we have examined the role of microtubule motors in transport carrier motility, morphology, and domain organization during ER-to-Golgi transport. We show that, consistent with our findings for endosomal dynamics, microtubule motor function during ER-to-Golgi transport of secretory cargo is required for motility, morphology, and cargo sorting within vesicular tubular carriers en route to the Golgi. Our data are consistent with previous findings that defined roles for dynein-1, kinesin-1 (KIF5B and kinesin-2 in this trafficking step. Our high resolution tracking data identify some intriguing aspects. Depletion of kinesin-1 reduces the number of motile structures seen, which is in line with other findings relating to the role of kinesin-1 in ER export. However, those transport carriers that were produced had a much greater run length suggesting that this motor can act as a brake on anterograde motility. Kinesin-2 depletion did not significantly reduce the number of motile transport carriers but did cause a similar increase in run length. These data suggest that kinesins act as negative regulators of ER-to-Golgi transport. Depletion of dynein not only reduced the number of motile carriers formed but also caused tubulation of carriers similar to that seen for sorting nexin-coated early endosomes. Our data indicated that the previously observed anterograde–retrograde polarity of transport carriers in transit to the Golgi from the ER is maintained by microtubule motor function.

  20. Comunidad, sistema de cargos y proyecto social. Una propuesta analítica de sociedades locales en México

    Directory of Open Access Journals (Sweden)

    Íñigo González de la Fuente

    2011-01-01

    Full Text Available El presente texto contiene una propuesta de análisis antropológico del sistema de cargos que incluye tanto a comunidades indígenas como no-indígenas. Partiendo del reconocimiento del sistema de cargos como una institución esencialmente heterogénea, se sugiere que, como hipótesis principal, tal institución alimenta una serie de mecanismos que, en sí mismos, potencian los escenarios de interacciones que protagonizan los miembros de la comunidad a través de un conjunto de roles: fomentan que los escenarios puedan ser protagonizados por el mayor número de miembros; propician que tales escenarios se articulen con la mayor frecuencia posible; todo ello con el acuerdo tácito entre protagonistas de que exista rotación de roles entre escenario y escenario. Esta propuesta analítica va a estar estructurada en tres bloques. En primer lugar, se ubican los fenómenos estudiados en torno al sistema de cargos en un continuum de comportamientos tendentes al individualismo y a lo colectivo en sus dos polos. En segundo lugar, se explicita el concepto de comunidad como elemento básico para el estudio de los sistemas de cargos; por último, se propone la idea del proyecto comunitario como denominador común en cuanto a sistemas de cargos se refiere en el área mesoamericana.

  1. Nuclear Car Wash sensitivity in varying thicknesses of wood and steel cargo

    Energy Technology Data Exchange (ETDEWEB)

    Church, J; Slaughter, D; Asztalos, S; Biltoft, P; Descalle, M; Hall, J; Manatt, D; Mauger, J; Norman, E; Petersen, D; Prussin, S

    2006-10-05

    The influence of incident neutron attenuation on signal strengths in the Nuclear Car Wash has been observed experimentally for both wood and steel-pipe mock cargos. Measured decay curves are presented for {beta}-delayed high-energy {gamma}-rays and thermalized neutrons following neutron-induced fission of HEU through varying irradiation lengths. Error rates are extracted for delayed-{gamma} and delayed-n signals integrated to 30 seconds, assuming Gaussian distributions for the active background. The extrapolation to a field system of 1 mA deuterium current and to a 5 kg sample size is discussed.

  2. Sandwich design of a lift plate for tail lift platforms for cargo trucks

    OpenAIRE

    Bergwall, Robert

    2015-01-01

    This master thesis examines a sandwich lift plate for cargo trucks in order to propose an option for existing lift platforms in aluminium or steel. The wish is to make it lighter but not less stiff or weak. Two different cores in Divinycell with different density; H130 and H250 is examined. Calculations are made with respect to deflection and max stress in both core and faces for the whole plates. But also more local calculations are performed to see how the faces and core behaved on local po...

  3. DISEÑO ESTRATÉGICO DE PERFILES DE CARGOS POR COMPETENCIAS.

    OpenAIRE

    Ariel Soltura Laseria; Armando Cuesta Santos

    2008-01-01

    En el trabajo se presenta una tecnología para la determinación de los perfiles de cargo por competencias en las organizaciones cubanas con base científica, fundamentada en la creciente necesidad de dirigir los esfuerzos individuales hacia el cumplimiento de la estrategia. La tecnología provee a las organizaciones de una forma de determinar la estructura de competencias que ha de adoptar para satisfacer las demandas o necesidades de clientes o usuarios, estado, proveedores y sociedad, en ...

  4. Determination of Inertia Forces Acting on Break Bulk Cargo en Route

    Directory of Open Access Journals (Sweden)

    A.O. Chepok

    2013-12-01

    Full Text Available The paper presents the analytical method of defining inertia forces that act on break bulk cargo as a result of the oscillatory motion of the vessel exposed to the effect of ambient forces. Considering that the linear models of roll, pitch and heave applicable in this case, the problem is solved by expressing the angle of heel, the angle of pitch, and the amplitude of heave. The obtained functions are differentiated and the inertia forces are determined by means of applying the Newton's second law.

  5. Fast neutron radiography scanner for the detection of contraband in air cargo containers

    International Nuclear Information System (INIS)

    There is a growing need to rapidly scan bulk air cargo for contraband such as illicit drugs and explosives. The Commonwealth Science and Industrial Research Organisation (CSIRO) have been working with Australian Customs Service to develop a scanner capable of directly scanning airfreight containers in 1-2 minutes without unpacking. The scanner combines fast neutron and gamma-ray radiography to provide high-resolution images that include information on material composition. A full-scale prototype scanner has been successfully tested in the laboratory and a commercial-scale scanner is due to be installed at Brisbane airport in 2005

  6. Waste Crate and Container Imaging Using the Vehicle and Cargo Inspection System. Innovative Technology Summary Report

    International Nuclear Information System (INIS)

    The Vehicle and Cargo Inspection System (VACIS) is a highly penetrating gamma ray imaging system that provides a means to non-invasively image crate contents prior to crate disassembly. The VACIS unit uses a 1.6 Curie collimated source (Cesium-137) aimed at a linear detector to create an image as the unit passes by the crate. In the demonstrated mobile unit, the source and detector were mounted on a boom truck. As the crate passed between the source and detector, a near real-time composite image of the contents was constructed from the linear image of the VACIS unit's on board computer and recorded on disk

  7. Cargo transport by sea and road - Technical and economical environmental factors

    DEFF Research Database (Denmark)

    Kristensen, Hans Otto Holmegaard

    2006-01-01

    .)) Having established a method for the calculation of the ships energy demand, it is relatively simple to calculate the exhaust emissions by using some well-established specific emission factors, i.e. figures for the exhaust emission pr. consumed energy unit (g/Mj). >> In the paper, a comparison with road...... transport will also be presented focusing on the energy demand and exhaust emissions per transport unit, i.e. per ton cargo per km. >>) Finally, the energy and emission data will be used for an economical evaluation of sea transport versus land transport. This will be done by calculating the external costs...

  8. X-ray and neutron interrogation of air cargo for mobile applications

    International Nuclear Information System (INIS)

    A system for scanning break-bulk cargo for mobile applications is presented. This combines a 140 kV multi-view, multi-energy X-ray system with 2.5 MeV neutrons. The system uses dual energy X-ray radiography with neutron radiography. The X-ray and neutron systems were designed to be collocated in a mobile environment. Various materials were interrogated with the intent of distinguishing threat materials such as explosives from similar benign materials. In particular, the identification of threats and bengins with nearly identical effective atomic numbers has been demonstrated

  9. Waste Crate and Container Imaging Using the Vehicle and Cargo Inspection System. Innovative Technology Summary Report

    Energy Technology Data Exchange (ETDEWEB)

    None

    2000-07-01

    The Vehicle and Cargo Inspection System (VACIS) is a highly penetrating gamma ray imaging system that provides a means to non-invasively image crate contents prior to crate disassembly. The VACIS unit uses a 1.6 Curie collimated source (Cesium-137) aimed at a linear detector to create an image as the unit passes by the crate. In the demonstrated mobile unit, the source and detector were mounted on a boom truck. As the crate passed between the source and detector, a near real-time composite image of the contents was constructed from the linear image of the VACIS unit's on board computer and recorded on disk.

  10. Economic Feasibility of an Air Cargo Handling Facility at Fargo, North Dakota

    OpenAIRE

    Golz, Theresa K.; Golz, Joel T.; Koo, Won W.

    1992-01-01

    Exports to Taiwan in 1990 via air freight were approximately $2.9 billion, and imports via air freight to the united States were $3.1 billion. Total U.S. exports/imports to/from Taiwan in 1990 were $11.5 billion and $22.7 billion, respectively. China Airlines operates three air cargo hubs in the united States: New York, Los Angeles, and Dallas. The real per capita Gross National Product (GNP) in Taiwan for 1989 was $7,512, up from $3,297 in 1985. This implies that the Taiwanese economy is one...

  11. How efficient is towing a cargo by a micro-swimmer?

    CERN Document Server

    Raz, Oren

    2008-01-01

    We study the properties of arbitrary micro-swimmers towing a passive load through a viscous liquid. The simple close-form expression for the dragging efficiency of a general micro-swimmer dragging a distant load is found, and the leading order approximation for finite mutual separation is derived. We show that, while swimmer can be arbitrarily efficient, dragging efficiency is always bounded from above. It is also demonstrated, that opposite to Purcell's assumption, the hydrodynamic coupling can ''help" the swimmer to drag the load. We support our conclusions by rigorous numerical calculations for the "necklace-shaped" swimmer, towing a spherical cargo positioned at a finite distance.

  12. The Emp24 complex recruits a specific cargo molecule into endoplasmic reticulum-derived vesicles

    OpenAIRE

    Muniz, M.; Nuoffer, C; Hauri, H P; Riezman, H

    2000-01-01

    Members of the yeast p24 family, including Emp24p and Erv25p, form a heteromeric complex re- quired for the efficient transport of selected proteins from the endoplasmic reticulum (ER) to the Golgi ap- paratus. The specific functions and sites of action of this complex are unknown. We show that Emp24p is di- rectly required for efficient packaging of a lumenal cargo protein, Gas1p, into ER-derived vesicles. Emp24p and Erv25p can be directly cross-linked to Gas...

  13. X-ray and neutron interrogation of air cargo for mobile applications

    Science.gov (United States)

    Van Liew, Seth

    2015-06-01

    A system for scanning break-bulk cargo for mobile applications is presented. This combines a 140 kV multi-view, multi-energy X-ray system with 2.5 MeV neutrons. The system uses dual energy X-ray radiography with neutron radiography. The X-ray and neutron systems were designed to be collocated in a mobile environment. Various materials were interrogated with the intent of distinguishing threat materials such as explosives from similar benign materials. In particular, the identification of threats and bengins with nearly identical effective atomic numbers has been demonstrated.

  14. Foundation for Heavy Lift: Early Developments in the Ares V Cargo Launch Vehicle

    Science.gov (United States)

    Sumrall, John P.; McArthur, J. Craig

    2007-01-01

    The Ares V Cargo Launch Vehicle (CaLV) is NASA's primary vessel for safe, reliable delivery of the Lunar Surface Access Module (LSAM) and other resources into Earth orbit, as articulated in the U.S. Vision for Space Exploration.' The Ares V launch concept is shown. The foundation for this heavy-lift companion to the Ares I Crew Launch Vehicle (CLV) is taking shape within NASA and with its government and industry partners. This paper will address accomplishments in the Ares V Launch Vehicle during 2006 and 2007 and offer a preview of future activities.

  15. Mission Design Considerations for Mars Cargo of the Human Spaceflight Architecture Team's Evolvable Mars Campaign

    Science.gov (United States)

    Sjauw, Waldy K.; McGuire, Melissa L.; Freeh, Joshua E.

    2016-01-01

    Recent NASA interest in human missions to Mars has led to an Evolvable Mars Campaign by the agency's Human Architecture Team. Delivering the crew return propulsion stages and Mars surface landers, SEP based systems are employed because of their high specific impulse characteristics enabling missions requiring less propellant although with longer transfer times. The Earth departure trajectories start from an SLS launch vehicle delivery orbit and are spiral shaped because of the low SEP thrust. Previous studies have led to interest in assessing the divide in trip time between the Earth departure and interplanetary legs of the mission for a representative SEP cargo vehicle.

  16. Microtubule-binding protein doublecortin-like kinase 1 (DCLK1) guides kinesin-3-mediated cargo transport to dendrites.

    Science.gov (United States)

    Lipka, Joanna; Kapitein, Lukas C; Jaworski, Jacek; Hoogenraad, Casper C

    2016-02-01

    In neurons, the polarized distribution of vesicles and other cellular materials is established through molecular motors that steer selective transport between axons and dendrites. It is currently unclear whether interactions between kinesin motors and microtubule-binding proteins can steer polarized transport. By screening all 45 kinesin family members, we systematically addressed which kinesin motors can translocate cargo in living cells and drive polarized transport in hippocampal neurons. While the majority of kinesin motors transport cargo selectively into axons, we identified five members of the kinesin-3 (KIF1) and kinesin-4 (KIF21) subfamily that can also target dendrites. We found that microtubule-binding protein doublecortin-like kinase 1 (DCLK1) labels a subset of dendritic microtubules and is required for KIF1-dependent dense-core vesicles (DCVs) trafficking into dendrites and dendrite development. Our study demonstrates that microtubule-binding proteins can provide local signals for specific kinesin motors to drive polarized cargo transport. PMID:26758546

  17. Monte-Carlo Simulations of Radiation-Induced Activation in a Fast-Neutron and Gamma- Based Cargo Inspection System

    CERN Document Server

    Bromberger, B; Brandis, M; Dangendorf, V; Goldberg, M B; Kaufmann, F; Mor, I; Nolte, R; Schmiedel, M; Tittelmeier, K; Vartsky, D; Wershofen, H

    2012-01-01

    An air cargo inspection system combining two nuclear reaction based techniques, namely Fast-Neutron Resonance Radiography and Dual-Discrete-Energy Gamma Radiography is currently being developed. This system is expected to allow detection of standard and improvised explosives as well as special nuclear materials. An important aspect for the applicability of nuclear techniques in an airport inspection facility is the inventory and lifetimes of radioactive isotopes produced by the neutron and gamma radiation inside the cargo, as well as the dose delivered by these isotopes to people in contact with the cargo during and following the interrogation procedure. Using MCNPX and CINDER90 we have calculated the activation levels for several typical inspection scenarios. One example is the activation of various metal samples embedded in a cotton-filled container. To validate the simulation results, a benchmark experiment was performed, in which metal samples were activated by fast-neutrons in a water-filled glass jar. T...

  18. La confiabilidad en los sistemas eléctricos competitivos y el modelo colombiano de cargo por confiabilidad

    Directory of Open Access Journals (Sweden)

    Vélez Luis Guillermo

    2012-09-01

    Full Text Available El propósito de este artículo es hacer un análisis de la seguridad en el suministro de electricidad en Colombia, considerando el nuevo esquema del Cargo por Confiabilidad, modelo adoptado en Colombia para garantizar las inversiones en generación. Para esto se analiza la diferencia entre escenarios de demanda y de oferta de electricidad. La oferta para las plantas nuevas considera las OEF adquiridas con el cargo; y para las plantas existentes se realizan simulaciones bajo diversos escenarios de ocurrencia del fenómeno de “El Niño”. La demanda fue tomada de las proyecciones de la UPME. Las simulaciones realizadas muestran una apropiada dimensión del tamaño del Cargo por Confiabilidad.

  19. Anomalous transport of subdiffusing cargos by single kinesin motors: the role of mechanochemical coupling and anharmonicity of tether

    CERN Document Server

    Goychuk, Igor

    2014-01-01

    Here we generalize our previous model of molecular motors trafficking subdiffusing cargos in viscoelastic cytosol by (i) including mechanochemical coupling between cyclic conformational fluctuations of the motor protein driven by the reaction of ATP hydrolysis and its translational motion within the simplest two-state model of hand-over-hand motion of kinesin, and also (ii) by taking into account the anharmonicity of the tether between the motor and cargo (its maximally possible extension length). It is shown that the major earlier results such as occurrence of normal versus anomalous transport depending on the amplitude of binding potential, cargo size and the motor turnover frequency not only survive in this more realistic model, but the results also look very similar for the correspondingly adjusted parameters. However, this more realistic model displays a substantially larger thermodynamic efficiency due to a bidirectional mechanochemical coupling. For realistic parameters, the maximal thermodynamic effic...

  20. A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes

    Science.gov (United States)

    Tie, Hieng Chiong; Mahajan, Divyanshu; Chen, Bing; Cheng, Li; VanDongen, Antonius M. J.; Lu, Lei

    2016-01-01

    Cellular functions of the Golgi are determined by the unique distribution of its resident proteins. Currently, electron microscopy is required for the localization of a Golgi protein at the sub-Golgi level. We developed a quantitative sub-Golgi localization method based on centers of fluorescence masses of nocodazole-induced Golgi ministacks under conventional optical microscopy. Our method is rapid, convenient, and quantitative, and it yields a practical localization resolution of ∼30 nm. The method was validated by the previous electron microscopy data. We quantitatively studied the intra-Golgi trafficking of synchronized secretory membrane cargoes and directly demonstrated the cisternal progression of cargoes from the cis- to the trans-Golgi. Our data suggest that the constitutive efflux of secretory cargoes could be restricted at the Golgi stack, and the entry of the trans-Golgi network in secretory pathway could be signal dependent. PMID:26764092

  1. Design of an experiment to measure the fire exposure of radioactive materials packages aboard container cargo ships

    International Nuclear Information System (INIS)

    The test described in this paper is intended to measure the typical accident environment for a radioactive materials package in a fire aboard a container cargo ship. A stack of nine used standard cargo containers will be variously loaded with empty packages, simulated packages and combustible cargo and placed over a large hydrocarbon pool fire of one hour duration. Both internal and external fire container fire environments typical of on-deck stowage will be measured as well as the potential for container to container fire spread. With the use of the inverse heat conduction calculations, the local heat transfer to the simulated packages can be estimated from thermocouple data. Data recorded will also provide information on fire durations in each container, fire intensity and container to container fire spread characteristics

  2. Design of an experiment to measure the fire exposure of radioactive materials packages aboard container cargo ships

    Energy Technology Data Exchange (ETDEWEB)

    Koski, J.A.

    1997-11-01

    The test described in this paper is intended to measure the typical accident environment for a radioactive materials package in a fire aboard a container cargo ship. A stack of nine used standard cargo containers will be variously loaded with empty packages, simulated packages and combustible cargo and placed over a large hydrocarbon pool fire of one hour duration. Both internal and external fire container fire environments typical of on-deck stowage will be measured as well as the potential for container to container fire spread. With the use of the inverse heat conduction calculations, the local heat transfer to the simulated packages can be estimated from thermocouple data. Data recorded will also provide information on fire durations in each container, fire intensity and container to container fire spread characteristics.

  3. Cargo Commercial Orbital Transportation Services Environmental Control and Life Support Integration

    Science.gov (United States)

    Duchesne, Stephanie; Thacker, Karen; Williams, Dave

    2012-01-01

    The International Space Station s (ISS) largest crew and cargo resupply vehicle, the Space Shuttle, retired in 2011. To help augment ISS resupply and return capability, NASA announced a project to promote the development of Commercial Orbital Transportation Services (COTS) for the ISS in January of 2006. By December of 2008, NASA entered into space act agreements with SpaceX and Orbital Sciences Corporation for COTS development and ISS Commercial Resupply Services (CRS). The intent of CRS is to fly multiple resupply missions each year to ISS with SpaceX s Dragon vehicle providing resupply and return capabilities and Orbital Science Corporation s Cygnus vehicle providing resupply capability to ISS. The ISS program launched an integration effort to ensure that these new commercial vehicles met the requirements of the ISS vehicle and ISS program needs. The Environmental Control and Life Support System (ECLSS) requirements cover basic cargo vehicle needs including maintaining atmosphere, providing atmosphere circulation, and fire detection and suppression. The ISS-COTS integration effort brought unique challenges combining NASA s established processes and design knowledge with the commercial companies new initiatives and limited experience with human space flight. This paper will discuss the ISS ECLS COTS integration effort including challenges, successes, and lessons learned.

  4. Mesoporous silica-supported lipid bilayers (protocells) for DNA cargo delivery to the spinal cord.

    Science.gov (United States)

    Dengler, Ellen C; Liu, Juewen; Kerwin, Audra; Torres, Sergio; Olcott, Clara M; Bowman, Brandi N; Armijo, Leisha; Gentry, Katherine; Wilkerson, Jenny; Wallace, James; Jiang, Xingmao; Carnes, Eric C; Brinker, C Jeffrey; Milligan, Erin D

    2013-06-10

    Amorphous mesoporous silica nanoparticles ('protocells') that support surface lipid bilayers recently characterized in vitro as carrier constructs for small drug and DNA delivery are reported here as highly biocompatible both in vitro and in vivo, involving the brain and spinal cord following spinal delivery into the lumbosacral subarachnoid space (intrathecal; i.t.). Specifically, positively charged, 1, 2-Dioleoyl-3-Trimethylammonium-Propane (DOTAP)-cholesterol (DOTAP:Chol) liposome-formulated protocells revealed stable in vitro cargo release kinetics and cellular interleukin-10 (IL-10) transgene transfection. Recent approaches using synthetic non-viral vector platforms to deliver the pain-suppressive therapeutic transgene, IL-10, to the spinal subarachnoid space have yielded promising results in animal models of peripheral neuropathy, a condition involving aberrant neuronal communication within sensory pathways in the nervous system. Non-viral drug and gene delivery protocell platforms offer potential flexibility because cargo release-rates can be pH-dependent. We report here that i.t. delivery of protocells, with modified chemistry supporting a surface coating of DOTAP:Chol liposomes and containing the IL-10 transgene, results in functional suppression of pain-related behavior in rats for extended periods. This study is the first demonstration that protocell vectors offer amenable and enduring in vivo biological characteristics that can be applied to spinal gene delivery. PMID:23517784

  5. PERANCANGAN KAPAL GENERAL CARGO 1500 DWT RUTE PELAYARAN JAKARTA-SURABAYA

    Directory of Open Access Journals (Sweden)

    Parlindungan Manik

    2013-06-01

    Full Text Available Kapal general cargo  adalah kapal yang mengangkut bermacam-macam muatan berupa barang.Barang yang diangkut biasanya merupakan barang yang sudah dikemas.Kapal general cargo dilengkapi dengan crane pengangkut barang untuk memudahkan bongkar-muat muatan. Pada penelitian ini direncanakan desain lambung kapal. Ukuran utama kapal didapatkan dengan menggunakan metode regresi yang didasarkan pada data 5 kapal pembanding. Dari ukuran utama yang didapat kemudian dilakukan pembuatan rencana garis, rencana umum, analisa hidrostatik, analisa stabilitas dan analisa olah gerak kapal yang sesuai dengan standar IMO. Setelah dilakukan analisa dengan menggunakan metode regresi yang didasarkan pada data 5 kapal pembanding yang diambil dari Register Of Ship2002-03LR (Lloyd’s Register dihasilkan alternatif ukuran utama kapal, yaitu dengan panjang kapal keseluruhan (Loa = 62,46 m, panjang kapal (Lpp = 57,95 m, lebar kapal (B = 12,26 m, tinggi kapal (H = 6,49 m, sarat kapal (T = 3,85 m, dan kecepatan kapal (Vd = 11 knot. Pada kecepatan maksimal 11 knot hambatan yang di terima sebesar = 66,76 KN dan power sebesar 506,3667 Hp. Nilai GZ  maksimum kapal = 3,369 m.deg dan nilai GM awal = 4,021 m.Gerakan heaving maksimum = 1,086 m, gerakan rolling maksimum =14,15°, dan gerakan pitching maksimum = 3,02°

  6. Feasibility Study of Cargo Airship Transportation Systems Powered by New Green Energy Technologies

    Science.gov (United States)

    Skuza, Jonathan R.; Park, Yeonjoon; Kim, Hyun Jung; Seaman, Shane T.; King, Glen C.; Choi, Sang H.; Song, Kyo D.; Yoon, Hargsoon; Lee, Kunik

    2014-01-01

    The development of transportation systems that use new and sustainable energy technologies is of utmost importance due to the possible future shortfalls that current transportation modes will encounter because of increased volume and costs. The introduction and further research and development of new transportation and energy systems by materials researchers at the National Aeronautics and Space Administration (NASA) Langley Research Center (LaRC) and the Department of Transportation are discussed in this Technical Memorandum. In this preliminary study, airship concepts were assessed for cargo transportation using various green energy technologies capable of 24-hour operation (i.e., night and day). Two prototype airships were successfully constructed and tested at LaRC to demonstrate their feasibility: one with commercially available solar cells for operation during the daytime and one with microwave rectennas (i.e., rectifying antennas) developed in-house for night-time operation. The test results indicate the feasibility of a cargo transportation airship powered by new green energy sources and wireless power technology. Future applications will exploit new green energy sources that use materials and devices recently developed or are in the process of being developed at LaRC. These include quantum well SiGe solar cells; low, mid-, and high temperature thermoelectric modules; and wireless microwave and optical rectenna devices. This study examines the need and development of new energy sources for transportation, including the current status of research, materials, and potential applications.

  7. Innovative nuclear reactors and fuel cycles for high-speed cargo vessels

    International Nuclear Information System (INIS)

    It has been about four decades since nuclear powered cargo ships were seriously discussed in the naval architecture community. Recent developments in commercial shipping include faster, bigger, and more powerful ships. In addition, several initiatives in advanced reactor design include smaller, safer and more efficient reactors. The development of advanced ships and advanced reactors opens an opportunity for nuclear propulsion. Although the goals of the INPRO and GIF Projects are not related to nuclear propulsion, these are analyzed for such potential case, considering the contribution to energy sustainability. These projects would help to select nuclear propulsion plant and fuel cycle concepts based on user requirements. Current technology trends point out at IPWRs and GT-MHRs, preferably the latter for overall advanced ship competitiveness. Core residual heat removal and other factors recommend the use of a hybrid power plant, with an auxiliary fossil fuel plant. This article illustrates future trends in high-performance cargo vessels, and the benefits of nuclear propulsion in such ships. Two advanced ship proposals are discussed for nuclear power, based on data availability and market opportunities. The economic viability of hybrid propulsion plants is anticipated, even with conservative reactor cost figures. The most important restriction is the reactor cost, so that these projects are opportune. (author)

  8. The bacterial carbon-fixing organelle is formed by shell envelopment of preassembled cargo.

    Directory of Open Access Journals (Sweden)

    Anna H Chen

    Full Text Available BACKGROUND: Cyanobacteria play a significant role in the global carbon cycle. In Synechococcuselongatus, the carbon-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO is concentrated into polyhedral, proteinaceous compartments called carboxysomes. METHODOLOGY/PRINCIPAL FINDINGS: Using live cell fluorescence microscopy, we show that carboxysomes are first detected as small seeds of RuBisCO that colocalize with existing carboxysomes. These seeds contain little or no shell protein, but increase in RuBisCO content over several hours, during which time they are exposed to the solvent. The maturing seed is then enclosed by shell proteins, a rapid process that seals RuBisCO from the cytosol to establish a distinct, solvent-protected microenvironment that is oxidizing relative to the cytosol. These closure events can be spatially and temporally coincident with the appearance of a nascent daughter RuBisCO seed. CONCLUSIONS/SIGNIFICANCE: Carboxysomes assemble in a stepwise fashion, inside-to-outside, revealing that cargo is the principle organizer of this compartment's biogenesis. Our observations of the spatial relationship of seeds to previously formed carboxysomes lead us to propose a model for carboxysome replication via sequential fission, polymerization, and encapsulation of their internal cargo.

  9. Scanning of Cargo Containers by Gamma-Ray and Fast Neutron Radiography

    International Nuclear Information System (INIS)

    This paper describes the combined systems which were installed and tested to detect contraband smuggled in cargo containers. These combined systems are based on radiographers work by gamma-rays emitted from point source 60Co with 0.5 Ci activity and neutrons emitted from point isotopic sources of Pu-α-Be as well as 14 MeV neutrons emitted from sealed tube neutron generator. The transmitted gamma ray through the inspected object was measured by gamma detection system with NaI(Tl) detector while the transmitted fast neutron beam was measured by a neutron gamma detection system with stilbene organic scintillator. The later possess the capability of discrimination between between gamma and neutron pulses using a discrimination system based on pulse shape discrimination method. The measured intensities of primary incident and transmitted beams of gamma-rays and fast neutrons were used to construct 2D cross-sectional images of the inspected objects hidden directly within benign materials of the container and for object screened by high dense material to stop object detection by gamma or X-rays. The constructed images for the inspected objects show the good capability and effectiveness of the installed gamma and neutron radiographers to detect illicit materials hidden in air cargo containers and sea containers of med size. They have also indicated that the developed scanning systems possess the ease of mobility and low cost of scanning

  10. The EURITRACK project: Status of a tagged neutron inspection system for cargo containers

    International Nuclear Information System (INIS)

    The EURopean Illicit TRAfficking Countermeasures Kit project is part of the 6th EU Framework program, and aims at developing a neutron inspection system for detecting threat materials in cargo containers. Neutron interaction in the container produces specific gamma-rays used to determine the chemical composition of the inspected material. The use of D+T tagged neutrons allows the inspection of a suspect voxel identified by previous x-ray scan. The EURITRACK project consists in developing: a transportable deuterium-tritium neutron generator including a position sensitive alpha detector (8x8 matrix of YAP:Ce crystals), fast neutron and gamma-ray detectors, front-end electronics to perform coincidence and spectroscopic measurements, and an integrated software which manage detector positioning, data acquisition, processing and analysis. All components have been developed and tested in Laboratory condition with neutron beams, their integration in the final portal will start in September 2006, whereas a field demonstration in a European seaport is planned for 2007. The provisional performances of the system were assessed by Monte Carlo calculations validated by experiments in which elemental or complex target were detected in cargo containers filled with different matrices (Fe-based or wooden). The status of the EURITRACK project will be presented. (author)

  11. DEVELOPMENT OF DECISION MAKING ALGORITHM FOR CONTROL OF SEA CARGO CONTAINERS BY 'TAGGED' NEUTRON METHOD

    International Nuclear Information System (INIS)

    Nowadays in Russia and abroad there are several groups of scientists, engaged in development of systems based on 'tagged' neutron method (API method) and intended for detection of dangerous materials, including high explosives (HE). Particular attention is paid to possibility of detection of dangerous objects inside a sea cargo container. Energy gamma-spectrum, registered from object under inspection is used for determination of oxygen/carbon and nitrogen/carbon chemical ratios, according to which dangerous object is distinguished from not dangerous one. Material of filled container, however, gives rise to additional effects of rescattering and moderation of 14 MeV primary neutrons of generator, attenuation of secondary gamma-radiation from reactions of inelastic neutron scattering on objects under inspection. These effects lead to distortion of energy gamma-response from examined object and therefore prevent correct recognition of chemical ratios. These difficulties are taken into account in analytical method, presented in the paper. Method has been validated against experimental data, obtained by the system for HE detection in sea cargo, based on API method and developed in VNIIA. Influence of shielding materials on results of HE detection and identification is considered. Wood and iron were used as shielding materials. Results of method application for analysis of experimental data on HE simulator measurement (tetryl, trotyl, hexogen) are presented.

  12. A survey of propulsion options for cargo and piloted missions to Mars.

    Science.gov (United States)

    Sankaran, K; Cassady, L; Kodys, A D; Choueiri, E Y

    2004-05-01

    In this paper, high-power electric propulsion options are surveyed in the context of cargo and piloted missions to Mars. A low-thrust trajectory optimization program (raptor) is utilized to analyze this mission. Candidate thrusters are chosen based upon demonstrated performance in the laboratory. Hall, self-field magnetoplasmadynamic (MPDT), self-field lithium Lorentz force accelerator (LiLFA), arcjet, and applied-field LiLFA systems are considered for this mission. In this first phase of the study, all thrusters are assumed to operate at a single power level (regardless of the efficiency-power curve), and the thruster specific mass and power plant specific mass are taken to be the same for all systems. Under these assumptions, for a 7.5 MW, 60 mT payload, piloted mission, the self-field LiLFA results in the shortest trip time (340 days) with a reasonable propellant mass fraction of 57% (129 mT). For a 150 kW, 9 mT payload, cargo mission, both the applied-field LiLFA and the Hall thruster seem reasonable choices with propellant mass fractions of 42 to 45%(7 to 8 mT). The Hall thrusters provide better trip times (530-570 days) compared to the applied-field LiLFA (710 days) for the relatively less demanding mission. PMID:15220162

  13. Research on bulk-cargo-port berth assignment based on priority of resource allocation

    Directory of Open Access Journals (Sweden)

    Chunfang Guo

    2013-03-01

    Full Text Available Purpose: The purpose of this paper is to propose a Priority of Resource Allocation model about how to utilize the resources of the port efficiently, through the improvement of traditional ant colony algorithm, the ship-berth matching relation constraint matrix formed by ontology reasoning. Design/methodology/approach: Through questionnaires?Explore factor analysis (EFA and principal component analysis, the authors extract the importance of the goods, the importance of customers, and type of trade as the main factors of the ship operating priority. Then the authors combine berth assignment problem with the improved ant colony algorithm, and use the model to improve ship scheduling quality. Finally, the authors verify the model with physical data in a bulk-cargo-port in China. Findings: Test by the real data of bulk cargo port, it show that ships’ resource using priority and the length of waiting time are consistent; it indicates that the priority of resource allocation play a prominent role in improving ship scheduling quality. Research limitations: The questionnaires is limited in only one port group, more  related Influence factors should be considered to extend the conclusion. Practical implications: The Priority of Resource Allocation model in this paper can be used to improve the efficiency of the dynamic berth assignment. Originality: This paper makes the time of ship in port minimized as the optimization of key indicators and establishes a dynamic berth assignment model based on improved ant colony algorithm and the ontology reasoning model.

  14. Crystallization of Saccharomyces cerevisiae α-mannosidase, a cargo protein of the Cvt pathway

    International Nuclear Information System (INIS)

    Vacuolar α-mannosidase, a cargo protein of the cytoplasm-to-vacuole targeting pathway, has been expressed, purified and crystallized. Saccharomyces cerevisiae α-mannosidase (Ams1) is a cargo protein that is transported to the vacuole by the cytoplasm-to-vacuole targeting (Cvt) pathway during conditions of growth and by autophagy during conditions of starvation. After transport to the vacuole, Ams1 functions as a resident hydrolase. Ams1 has been overexpressed in the methylotrophic yeast Pichia pastoris, purified and crystallized in two crystal forms. Form I belongs to space group P21, with unit-cell parameters a = 145.7, b = 127.7, c = 164.0 Å, β = 101.5°. Form II belongs to space group I222 or I212121, with unit-cell parameters a = 127.9, b = 163.7, c = 291.5 Å. Diffraction data were collected from these crystals to a resolution of 3.3 Å for form I and of 2.6 Å for form II using synchrotron radiation

  15. Life Science on the International Space Station Using the Next Generation of Cargo Vehicles

    Science.gov (United States)

    Robinson, J. A.; Phillion, J. P.; Hart, A. T.; Comella, J.; Edeen, M.; Ruttley, T. M.

    2011-01-01

    With the retirement of the Space Shuttle and the transition of the International Space Station (ISS) from assembly to full laboratory capabilities, the opportunity to perform life science research in space has increased dramatically, while the operational considerations associated with transportation of the experiments has changed dramatically. US researchers have allocations on the European Automated Transfer Vehicle (ATV) and Japanese H-II Transfer Vehicle (HTV). In addition, the International Space Station (ISS) Cargo Resupply Services (CRS) contract will provide consumables and payloads to and from the ISS via the unmanned SpaceX (offers launch and return capabilities) and Orbital (offers only launch capabilities) resupply vehicles. Early requirements drove the capabilities of the vehicle providers; however, many other engineering considerations affect the actual design and operations plans. To better enable the use of the International Space Station as a National Laboratory, ground and on-orbit facility development can augment the vehicle capabilities to better support needs for cell biology, animal research, and conditioned sample return. NASA Life scientists with experience launching research on the space shuttle can find the trades between the capabilities of the many different vehicles to be confusing. In this presentation we will summarize vehicle and associated ground processing capabilities as well as key concepts of operations for different types of life sciences research being launched in the cargo vehicles. We will provide the latest status of vehicle capabilities and support hardware and facilities development being made to enable the broadest implementation of life sciences research on the ISS.

  16. The targeted delivery of multicomponent cargos to cancer cells by nanoporous particle-supported lipid bilayers

    Science.gov (United States)

    Ashley, Carlee E.; Carnes, Eric C.; Phillips, Genevieve K.; Padilla, David; Durfee, Paul N.; Brown, Page A.; Hanna, Tracey N.; Liu, Juewen; Phillips, Brandy; Carter, Mark B.; Carroll, Nick J.; Jiang, Xingmao; Dunphy, Darren R.; Willman, Cheryl L.; Petsev, Dimiter N.; Evans, Deborah G.; Parikh, Atul N.; Chackerian, Bryce; Wharton, Walker; Peabody, David S.; Brinker, C. Jeffrey

    2011-05-01

    Encapsulation of drugs within nanocarriers that selectively target malignant cells promises to mitigate side effects of conventional chemotherapy and to enable delivery of the unique drug combinations needed for personalized medicine. To realize this potential, however, targeted nanocarriers must simultaneously overcome multiple challenges, including specificity, stability and a high capacity for disparate cargos. Here we report porous nanoparticle-supported lipid bilayers (protocells) that synergistically combine properties of liposomes and nanoporous particles. Protocells modified with a targeting peptide that binds to human hepatocellular carcinoma exhibit a 10,000-fold greater affinity for human hepatocellular carcinoma than for hepatocytes, endothelial cells or immune cells. Furthermore, protocells can be loaded with combinations of therapeutic (drugs, small interfering RNA and toxins) and diagnostic (quantum dots) agents and modified to promote endosomal escape and nuclear accumulation of selected cargos. The enormous capacity of the high-surface-area nanoporous core combined with the enhanced targeting efficacy enabled by the fluid supported lipid bilayer enable a single protocell loaded with a drug cocktail to kill a drug-resistant human hepatocellular carcinoma cell, representing a 106-fold improvement over comparable liposomes.

  17. The influence of direct motor–motor interaction in models for cargo transport by a single team of motors

    International Nuclear Information System (INIS)

    We analyze theoretically the effects of excluded-volume interactions between motors on the dynamics of a cargo driven by multiple motors. The model considered shares much in common with others recently proposed in the literature, with the addition of direct interaction between motors and motor back steps. The cargo is assumed to follow a continuum Langevin dynamics, while individual motors evolve following a Monte Carlo algorithm based on experimentally accessible probabilities for discrete forward and backward jumps, and attachment and detachment rates. The links between cargo and motors are considered as nonlinear springs. By means of numerical simulations we compute the relevant quantities characterizing the dynamical properties of the system, and we compare the results to those for noninteracting motors. We find that interactions lead to quite relevant changes in the force-velocity relation for cargo, with a considerable reduction of the stall force, and also cause a notable decrease of the run length. These effects are mainly due to traffic-like phenomena in the microtubule. The consideration of several parallel tracks for motors reduces such effects. However, we find that for realistic values of the number of motors and the number of tracks, the influence of interactions on the global parameters of transport of cargo are far from being negligible. Our studies also provide an analysis of the relevance of motor back steps on the modeling, and of the influence of different assumptions for the detachment rates. In particular, we discuss these two aspects in connection with the possibility of observing processive back motion of cargo at large load forces

  18. Evaluación de Riesgos Psicosociales en conductores de Altos Cargos de la Junta de Andalucía.

    OpenAIRE

    López-Zorzano, Fermín

    2013-01-01

    Este proyecto tiene por título “Evaluación de Riesgos Psicosociales en conductores de Altos cargos de la Junta de Andalucía”. Se trata de identificar los factores de riesgo y los niveles de riesgos asociados a estos, como puedan ser el estrés y los problemas asociados a éste, en los conductores de altos cargos de la Junta de Andalucía, para tratar de eliminarlos y poder establecer un programa de actuaciones, propuestas y medidas preventivas y/o correctivas para aquellos que no hayan podido el...

  19. Comunidad, sistema de cargos y proyecto social. Una propuesta analítica de sociedades locales en México

    OpenAIRE

    Íñigo González de la Fuente

    2011-01-01

    El presente texto contiene una propuesta de análisis antropológico del sistema de cargos que incluye tanto a comunidades indígenas como no-indígenas. Partiendo del reconocimiento del sistema de cargos como una institución esencialmente heterogénea, se sugiere que, como hipótesis principal, tal institución alimenta una serie de mecanismos que, en sí mismos, potencian los escenarios de interacciones que protagonizan los miembros de la comunidad a través de un conjunto de roles: fomentan que los...

  20. The distance that kinesin-1 holds its cargo from the microtubule surface measured by fluorescence interference contrast microscopy

    OpenAIRE

    Kerssemakers, Jacob; Howard, Jonathon; Hess, Henry; Diez, Stefan

    2006-01-01

    Kinesin-1 is a motor protein that carries cellular cargo such as membrane-bounded organelles along microtubules (MTs). The homodimeric motor molecule contains two N-terminal motor domains (the motor “heads”), a long coiled-coil domain (the “rod” or “stalk”), and two small globular “tail” domains. Much has been learned about how kinesin's heads step along a MT and how the tail is involved in cargo binding and autoinhibition. However, little is known about the role of the rod. Here, we investig...

  1. 49 CFR 172.331 - Bulk packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Bulk packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks. 172.331 Section 172.331 Transportation Other Regulations... packagings other than portable tanks, cargo tanks, tank cars and multi-unit tank car tanks. (a) Each...

  2. 46 CFR 32.50-35 - Remote manual shutdown for internal combustion engine driven cargo pump on tank vessels-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Remote manual shutdown for internal combustion engine... for Cargo Handling § 32.50-35 Remote manual shutdown for internal combustion engine driven cargo pump on tank vessels—TB/ALL. (a) Any tank vessel which is equipped with an internal combustion...

  3. Familiares a cargo de pacientes de cáncer: funciones y desafíos (PDQ®)—Versión para profesionales de salud

    Science.gov (United States)

    Sumario informativo revisado por expertos acerca de los desafíos que enfrentan los familiares a cargo de los pacientes con cáncer. Este resumen se centra en las funciones típicas y las inquietudes de las personas a cargo del paciente y en las intervenciones útiles para esas personas.

  4. Logistics worldwide for the whole nuclear cycle. Nuclear Cargo + Service GmbH; Weltweite Logistik im gesamten Nuklear-Kreislauf. Nuclear Cargo + Service GmbH

    Energy Technology Data Exchange (ETDEWEB)

    Geiger, Horst; Schymke, Klaus [Nuclear Cargo + Service GmbH, Hanau (Germany)

    2011-03-15

    Nuclear Cargo + Service GmbH (NCS) offers logistics worldwide for the whole nuclear cycle to fuel manufacturers, reactor operators, and waste management companies. Transport equipment and interim stores are part of the company's facilities. The firm's Front End unit conducts transnational transports of yellow cake, uranium hexafluoride, and enriched uranium and uranium from reprocessing. In the Back End unit, the company performs worldwide transports of spent fuel elements of research reactors. Another key area of activity is shipment of radioactive waste ranging from low-level waste to HAW (highly active waste generating heat). In its Component Disposal area, NCS offers a variety of transport services up to and including large components, e.g. reactor pressure vessels, both on the road and by rail and ship. NCS operates storage halls of its own for storing radioactive materials including radioactive waste. The technical equipment of NCS comprises a large pool of road and rail transport vehicles as well as a great number of vessels for radioactive materials. (orig.)

  5. 78 FR 11981 - Security Zone; Protection of Military Cargo, Captain of the Port Zone Puget Sound, WA

    Science.gov (United States)

    2013-02-21

    ... of Puget Sound and adjacent waters. (See 69 FR 52600, Aug. 27, 2004). When activated, this regulation... Puget Sound, WA AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The... military cargo in the navigable waters of Puget Sound and adjacent waters. Entry into this zone...

  6. Molecular Recognition of PTS-1 Cargo Proteins by Pex5p: Implications for Protein Mistargeting in Primary Hyperoxaluria

    Directory of Open Access Journals (Sweden)

    Noel Mesa-Torres

    2015-02-01

    Full Text Available Peroxisomal biogenesis and function critically depends on the import of cytosolic proteins carrying a PTS1 sequence into this organelle upon interaction with the peroxin Pex5p. Recent structural studies have provided important insights into the molecular recognition of cargo proteins by Pex5p. Peroxisomal import is a key feature in the pathogenesis of primary hyperoxaluria type 1 (PH1, where alanine:glyoxylate aminotransferase (AGT undergoes mitochondrial mistargeting in about a third of patients. Here, we study the molecular recognition of PTS1 cargo proteins by Pex5p using oligopeptides and AGT variants bearing different natural PTS1 sequences, and employing an array of biophysical, computational and cell biology techniques. Changes in affinity for Pex5p (spanning over 3–4 orders of magnitude reflect different thermodynamic signatures, but overall bury similar amounts of molecular surface. Structure/energetic analyses provide information on the contribution of ancillary regions and the conformational changes induced in Pex5p and the PTS1 cargo upon complex formation. Pex5p stability in vitro is enhanced upon cargo binding according to their binding affinities. Moreover, we provide evidence that the rational modulation of the AGT: Pex5p binding affinity might be useful tools to investigate mistargeting and misfolding in PH1 by pulling the folding equilibria towards the native and peroxisomal import competent state.

  7. 46 CFR 32.65-40 - Construction and testing of cargo tanks and bulkheads-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Construction and testing of cargo tanks and bulkheads-TB/ALL. 32.65-40 Section 32.65-40 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Hull Requirements for Tank Vessels Constructed On or After November 10, 1936, and Prior to July...

  8. Low cost, robust and real time system for detecting and tracking moving objects to automate cargo handling in port terminals

    NARCIS (Netherlands)

    Vaquero, V.; Repiso, E.; Sanfeliu, A.; Vissers, J.; Kwakkernaat, M.

    2016-01-01

    The presented paper addresses the problem of detecting and tracking moving objects for autonomous cargo handling in port terminals using a perception system which input data is a single layer laser scanner. A computationally low cost and robust Detection and Tracking Moving Objects (DATMO) algorithm

  9. Autonomous rendezvous and docking operations of unmanned expendable cargo transfer vehicles (e.g. Centaur) with Space Station Freedom

    Science.gov (United States)

    Emmet, Brian R.

    1991-01-01

    This paper describes the results of the feasibility study using Centaur or other CTV's to deliver payloads to the Space Station Freedom (SSF). During this study was examined the requirements upon unmanned cargo transfer stages (including Centaur) for phasing, rendezvous, proximity operations and docking/berthing (capture).

  10. 33 CFR 155.1035 - Response plan requirements for manned vessels carrying oil as a primary cargo.

    Science.gov (United States)

    2010-07-01

    ... for those notifications. Notifications must include those required by— (i) MARPOL 73/78 and 33 CFR...) Portable pumps and ancillary equipment; (D) Lightering and mooring masters (ship-to-ship only); and (E... the oil cargo aboard the vessel. A material safety data sheet meeting the requirements of 29 CFR...

  11. 19 CFR 10.41a - Lift vans, cargo vans, shipping tanks, skids, pallets, and similar instruments of international...

    Science.gov (United States)

    2010-04-01

    ... the List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and..., pallets, and similar instruments of international traffic; repair components. 10.41a Section 10.41a... Traffic § 10.41a Lift vans, cargo vans, shipping tanks, skids, pallets, and similar instruments...

  12. Pick-up, transport and release of a molecular cargo using a small-molecule robotic arm

    Science.gov (United States)

    Kassem, Salma; Lee, Alan T. L.; Leigh, David A.; Markevicius, Augustinas; Solà, Jordi

    2016-02-01

    Modern-day factory assembly lines often feature robots that pick up, reposition and connect components in a programmed manner. The idea of manipulating molecular fragments in a similar way has to date only been explored using biological building blocks (specifically DNA). Here, we report on a wholly artificial small-molecule robotic arm capable of selectively transporting a molecular cargo in either direction between two spatially distinct, chemically similar, sites on a molecular platform. The arm picks up/releases a 3-mercaptopropanehydrazide cargo by formation/breakage of a disulfide bond, while dynamic hydrazone chemistry controls the cargo binding to the platform. Transport is controlled by selectively inducing conformational and configurational changes within an embedded hydrazone rotary switch that steers the robotic arm. In a three-stage operation, 79-85% of 3-mercaptopropanehydrazide molecules are transported in either (chosen) direction between the two platform sites, without the cargo at any time fully dissociating from the machine nor exchanging with other molecules in the bulk.

  13. Preliminary investigations on a NTP cargo shuttle for earth to moon orbit payload transfer based on a particle bed reactor

    International Nuclear Information System (INIS)

    MAPS, a 3-year study program on NTP has recently been launched at CEA following the conclusions of a preliminary scoping study of an NTP system for earth to moon orbit cargo shuttle missions. This paper presents the main results of this scoping study, and gives an outline of the MAPS program. (authors). 5 figs., 11 tabs., 7 refs

  14. 31 CFR 585.215 - Conveyances and cargo suspected of being in violation of United Nations sanctions; detention...

    Science.gov (United States)

    2010-07-01

    ... being in violation of United Nations sanctions; detention; blocking. 585.215 Section 585.215 Money and... and cargo suspected of being in violation of United Nations sanctions; detention; blocking. (a) Except... § 585.201, but which are suspected of a violation of United Nations Security Council Resolutions No....

  15. Relationships between Cargo, Cell Penetrating Peptides and Cell Type for Uptake of Non-Covalent Complexes into Live Cells

    Directory of Open Access Journals (Sweden)

    Andrea-Anneliese Keller

    2013-02-01

    Full Text Available Modulating signaling pathways for research and therapy requires either suppression or expression of selected genes or internalization of proteins such as enzymes, antibodies, nucleotide binding proteins or substrates including nucleoside phosphates and enzyme inhibitors. Peptides, proteins and nucleotides are transported by fusing or conjugating them to cell penetrating peptides or by formation of non-covalent complexes. The latter is often preferred because of easy handling, uptake efficiency and auto-release of cargo into the live cell. In our studies complexes are formed with labeled or readily detectable cargoes for qualitative and quantitative estimation of their internalization. Properties and behavior of adhesion and suspension vertebrate cells as well as the protozoa Leishmania tarentolae are investigated with respect to proteolytic activity, uptake efficiency, intracellular localization and cytotoxicity. Our results show that peptide stability to membrane-bound, secreted or intracellular proteases varies between different CPPs and that the suitability of individual CPPs for a particular cargo in complex formation by non-covalent interactions requires detailed studies. Cells vary in their sensitivity to increasing concentrations of CPPs. Thus, most cells can be efficiently transduced with peptides, proteins and nucleotides with intracellular concentrations in the low micromole range. For each cargo, cell type and CPP the optimal conditions must be determined separately.

  16. 40 CFR Table 2 to Subpart Cccccc... - Applicability Criteria and Management Practices for Gasoline Cargo Tanks Unloading at Gasoline...

    Science.gov (United States)

    2010-07-01

    ... Practices for Gasoline Cargo Tanks Unloading at Gasoline Dispensing Facilities With Monthly Throughput of 100,000 Gallons of Gasoline or More 2 Table 2 to Subpart CCCCCC of Part 63 Protection of Environment... Pollutants for Source Category: Gasoline Dispensing Facilities Pt. 63, Subpt. CCCCCC, Table 2 Table 2...

  17. Anomalous transport of subdiffusing cargos by single kinesin motors: the role of mechano–chemical coupling and anharmonicity of tether

    International Nuclear Information System (INIS)

    Here we generalize our previous model of molecular motors trafficking subdiffusing cargos in viscoelastic cytosol by (i) including mechano–chemical coupling between cyclic conformational fluctuations of the motor protein driven by the reaction of ATP hydrolysis and its translational motion within the simplest two-state model of hand-over-hand motion of kinesin, and also (ii) by taking into account the anharmonicity of the tether between the motor and the cargo (its maximally possible extension length). It is shown that the major earlier results such as occurrence of normal versus anomalous transport depending on the amplitude of binding potential, cargo size and the motor turnover frequency not only survive in this more realistic model, but the results also look very similar for the correspondingly adjusted parameters. However, this more realistic model displays a substantially larger thermodynamic efficiency due to a bidirectional mechano–chemical coupling. For realistic parameters, the maximal thermodynamic efficiency can transiently be about 50% as observed for kinesins, and even larger, surprisingly also in a novel strongly anomalous (sub)transport regime, where the motor enzymatic turnovers become also anomalously slow and cannot be characterized by a turnover rate. Here anomalously slow dynamics of the cargo enforces anomalously slow cyclic kinetics of the motor protein. (paper)

  18. United Airlines Assists Tsunami Relief Effort United donates cargo space for relief supplies going to southern Asia

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ United Airlines today announced several initiatives to assist with the tsunami relief effort in Asia, including the donation of available cargo space to ship relief supplies to southern Thailand,Indonesia, Sri Lanka and other coastal areas on the Indian Ocean.

  19. United Airlines Assists Tsunami Relief Effort United donates cargo space for relief supplies going to southern Asia

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

      United Airlines today announced several initiatives to assist with the tsunami relief effort in Asia, including the donation of available cargo space to ship relief supplies to southern Thailand,Indonesia, Sri Lanka and other coastal areas on the Indian Ocean.……

  20. Perfiles de cargos basados en competencias para la empresa Fabega S.A.S.

    OpenAIRE

    Pérez Bolaños, Gretty Yohanna; Marulanda Vélez, Milena

    2012-01-01

    El presente trabajo está orientado a la estructuración de los perfiles de cargos administrativos y operativos basados en competencias, para la empresa FABEGA S.A.S – Planeta Sport, a través de métodos de investigación que permitan evaluar el estado actual e intervenir en los procesos, los cuales darán lugar a la implementación de los perfiles por competencias. Esta investigación se cimentará a partir del análisis y descripción de la problemática a resolver, la cual definirá los métodos, técn...

  1. Analysis and Design of a Long Range PTFE Substrate UHF RFID Tag for Cargo Container Identification

    Science.gov (United States)

    Petrariu, Adrian-Ioan; Popa, Valentin

    2016-01-01

    In this paper, a high-performances microstrip antenna for UHF (ultra high frequency) RFID (radio frequency identification) tag is designed, prototyped and tested. The antenna consists of two main components: a 1.52 mm RT/duroid 5880 laminate substrate on which the antenna is designed and a 10 mm polytetrafluoroethylene (PTFE) dielectric material placed as a separator between the antenna and the reference ground plane for the microstrip antenna. With this structure, the RFID tag can reach a maximum reading distance of 19 m, although the antenna has a compact size of 80 mm × 50 mm. The long reading distance is obtained by attaching to the antenna an RFID chip that can provide a reading sensitivity of -20.5 dBm. The high bandwidth from 677 MHz to 947 MHz measured at -10 dB, makes the tag being usable worldwide especially for cargo container identification, the main purpose of this research.

  2. RESEARCH ACTION: IMPLEMENTATION ZERO BASED BUDGET (ZBB IN THE PROVIDER SERVICE LEASING EQUIPAMENTS OF CARGO HANDLING.

    Directory of Open Access Journals (Sweden)

    Levi Gimenez

    2013-06-01

    Full Text Available This article aims to present the implementation of zero-based budgeting in a rental service provider of cargo handling equipment using the action research methodology. The goal was to examine the possibility of concomitant use of this instrument in service providers in need of accurate information that enables targeting at the best result in a setting avid for quick decisions and actions. Action research was used as research method. It was concluded that it is suitable for this branch, confirming its position as a useful model for restructuring and cutting costs, improving operational and financial results, and as a factor improving organizational environment (behavioral aspects, indirectly creating value to stakeholders.

  3. Design of an autonomous teleoperated cargo transporting vehicle for lunar base operations

    Science.gov (United States)

    Holt, James; Lao, Tom; Monali, Nkoy

    1989-01-01

    At the turn of the century NASA plans to begin construction of a lunar base. The base will likely consist of developed areas (i.e., habitation, laboratory, landing and launching sites, power plant) separated from each other due to safety considerations. The Self-Repositioning Track Vehicle (SRTV) was designed to transport cargo between these base facilities. The SRTV operates by using two robotic arms to raise and position segments of track upon which the vehicle travels. The SRTV utilizes the semiautonomous mobility (SAM) method of teleoperation; actuator-controlled interlocking track sections; two robotic arms each with five degrees of freedom; and these materials: titanium for structural members and aluminum for shell members, with the possible use of light-weight, high-strength composites.

  4. Introduction to Extracellular Vesicles: Biogenesis, RNA Cargo Selection, Content, Release, and Uptake.

    Science.gov (United States)

    Abels, Erik R; Breakefield, Xandra O

    2016-04-01

    Extracellular vesicles are a heterogeneous group of membrane-limited vesicles loaded with various proteins, lipids, and nucleic acids. Release of extracellular vesicles from its cell of origin occurs either through the outward budding of the plasma membrane or through the inward budding of the endosomal membrane, resulting in the formation of multivesicular bodies, which release vesicles upon fusion with the plasma membrane. The release of vesicles can facilitate intercellular communication by contact with or by internalization of contents, either by fusion with the plasma membrane or by endocytosis into "recipient" cells. Although the interest in extracellular vesicle research is increasing, there are still no real standards in place to separate or classify the different types of vesicles. This review provides an introduction into this expanding and complex field of research focusing on the biogenesis, nucleic acid cargo loading, content, release, and uptake of extracellular vesicles. PMID:27053351

  5. Molecular motors transporting cargos in viscoelastic cytosol: how to beat subdiffusion with a power stroke?

    CERN Document Server

    Goychuk, Igor; Metzler, Ralf

    2013-01-01

    Anomalously slow passive diffusion, $\\langle \\delta x^2(t)\\rangle\\simeq t^{\\alpha}$, with $0<\\alpha<1$, of larger tracers such as messenger RNA and endogenous submicron granules in the cytoplasm of living biological cells has been demonstrated in a number of experiments and has been attributed to the viscoelastic physical nature of the cellular cytoplasm. This finding provokes the question to which extent active intracellular transport is affected by this viscoelastic environment: does the subdiffusion of free submicron cargo such as vesicles and organelles always imply anomalously slow transport by molecular motors such as kinesins, that is, directed transport characterized by a sublinear growth of the mean distance, $\\langle x(t)\\rangle\\simeq t^{\\alpha_{\\rm eff}}$, with $0<\\alpha_{\\rm eff}<1$? Here we study a generic model approach combining the commonly accepted two-state Brownian ratchet model of kinesin motors based on the continuous-state diffusion along microtubule driven by a flashing bind...

  6. Cosmic-ray-induced ship-effect neutron measurements and implications for cargo scanning at borders

    International Nuclear Information System (INIS)

    Neutron measurements are used as part of the interdiction process for illicit nuclear materials at border crossings. Even though the natural neutron background is small, its variation can impact the sensitivity of detection systems. The natural background of neutrons that is observed in monitoring instruments arises almost entirely from cosmic-ray-induced cascades in the atmosphere and the surrounding environment. One significant source of variation in the observed neutron background is produced by the 'ship effect' in large quantities of cargo that transit past detection instruments. This paper reports on results from measurements with typical monitoring equipment of ship effect neutrons in various materials. One new result is the 'neutron shadow shielding' effect seen with some low neutron density materials

  7. The Nuclear Car Wash: Neutron interrogation of cargo containers to detect hidden SNM

    International Nuclear Information System (INIS)

    LLNL is actively involved in the development of advanced technologies for use in detecting threats in sea-going cargo containers, particularly the presence of hidden special nuclear materials (SNM). The 'Nuclear Car Wash' (NCW) project presented here uses a high-energy (E n ∼ 3.5-7.0 MeV) neutron probe to scan a container and then takes high-energy (E γ ≥ 2.5 MeV), β-delayed γ-rays emitted during the subsequent decay of any short-lived, neutron-induced fission products as a signature of fissionable material. The components of the proposed system (e.g. neutron source, gamma detectors, etc.) will be discussed along with data processing schemes, possible threat detection metrics and potential interference signals. Results from recent laboratory experiments using a prototype system at LLNL will also be presented

  8. The Nuclear Car Wash: Neutron interrogation of cargo containers to detect hidden SNM

    Science.gov (United States)

    Hall, J. M.; Asztalos, S.; Biltoft, P.; Church, J.; Descalle, M.-A.; Luu, T.; Manatt, D.; Mauger, G.; Norman, E.; Petersen, D.; Pruet, J.; Prussin, S.; Slaughter, D.

    2007-08-01

    LLNL is actively involved in the development of advanced technologies for use in detecting threats in sea-going cargo containers, particularly the presence of hidden special nuclear materials (SNM). The "Nuclear Car Wash" (NCW) project presented here uses a high-energy (En ≈ 3.5-7.0 MeV) neutron probe to scan a container and then takes high-energy (Eγ ⩾ 2.5 MeV), β-delayed γ-rays emitted during the subsequent decay of any short-lived, neutron-induced fission products as a signature of fissionable material. The components of the proposed system (e.g. neutron source, gamma detectors, etc.) will be discussed along with data processing schemes, possible threat detection metrics and potential interference signals. Results from recent laboratory experiments using a prototype system at LLNL will also be presented.

  9. Cube-octameric silsesquioxane-mediated cargo peptide delivery into living cancer cells.

    Science.gov (United States)

    Hörner, Sebastian; Fabritz, Sebastian; Herce, Henry D; Avrutina, Olga; Dietz, Christian; Stark, Robert W; Cardoso, M Cristina; Kolmar, Harald

    2013-04-14

    Cube octameric silsesquioxanes (COSS) are among the smallest nanoparticles known to date with a diameter of only 0.7 nm. We describe a COSS-based delivery system which allows for the drug targeting in human cells. It comprises a siloxane core with seven pendant aminopropyl groups and a fluorescently labeled peptidic ligand attached to one cage corner via a reversible disulfide bond to ensure its intracellular release. Bimodal amplitude-modulated atomic force microscopy (AFM) experiments revealed the formation of dendritic COSS structures by a self-assembly of single particles on negatively charged surfaces. Nuclear targeting was demonstrated in HeLa cells by selective binding of released p21(Cip1/Waf1)-derived cargo peptide to PCNA, a protein involved in DNA replication and repair. PMID:23250285

  10. Innovative nuclear reactors and fuel cycles for high-speed cargo vessels

    International Nuclear Information System (INIS)

    Full text: It has been about four decades since nuclear powered cargo ships were seriously discussed in the naval architecture community. However, recent developments in commercial shipping include faster, bigger, and more powerful ships. In addition, several initiatives in advanced reactor design include smaller, safer and more efficient reactors. The development of advanced ships and advanced reactors opens an opportunity for nuclear propulsion. Although the goals of the international projects, such as INPRO or GIF, are not directly related to propulsion, those are analyzed for such possibility regarding the contribution of nuclear propulsion to energy sustainability and environment, considering that freight and transportation activities currently fueled by oil or other fossil fuels will continue to expand. Nuclear energy only serves 6% of the primary energy mix. Current nuclear technology can provide large and lasting CO2-free energy with less harm to the environment and minimum depletion of natural energy resources. Contradictorily, its potential contribution to sustainable development is unlikely without a major technological shift, resulting in much less capital intensive power plants and reduced energy production costs for competitive operation, in addition to huge reactor and fuel cycle safety, waste and non-proliferation improvements. Early commercial marine nuclear power did not succeed. However, based on a set of 'marine' economics; environment, fuel cycle and waste; reactor and fuel cycle safety; non-proliferation and other crosscutting user requirements, and on cargo trends, innovative nuclear propulsion could be justified for a few ship classes: the high-performance cargo monohull and multihull, with innovative propulsors and highly compact power plant packages. Two tools were used for characterizing these platforms: the Suspension Pyramid and the Transport Factor. In some cases, the fuel component of the transport factor may be very large (and indeed

  11. Developing a model law restricting the transporting of passengers in the cargo areas of pickup trucks.

    Science.gov (United States)

    Christoffel, T; Agran, P; Winn, D; Anderson, C; Del Valle, C

    2000-01-01

    Pickup trucks have become increasingly popular in the United States, accounting for about one in five vehicles involved in fatal motor-vehicle crashes. A critical factor in these deaths is the practice of carrying passengers in truck cargo areas, which are not designed for this purpose. Each year approximately 200 deaths occur to occupants riding in the back of pickup trucks. Over half the states have laws dealing with preventable injury problem, but these laws vary widely and most are too limited to be effective. We have reviewed existing laws, as well as crash injury data, and we have developed and recommend a model bill. The bill focuses on intended use, restricting passengers to only those portions of a vehicle designed for human transport. We have also conducted and report on a survey of legislative sponsors of pickup truck legislation. PMID:10754798

  12. Engineering of bacterial exotoxins for highly efficient and receptor-specific intracellular delivery of diverse cargos.

    Science.gov (United States)

    Ryou, Jeong-Hyun; Sohn, Yoo-Kyoung; Hwang, Da-Eun; Park, Woo-Yong; Kim, Nury; Heo, Won-Do; Kim, Mi-Young; Kim, Hak-Sung

    2016-08-01

    The intracellular delivery of proteins with high efficiency in a receptor-specific manner is of great significance in molecular medicine and biotechnology, but remains a challenge. Herein, we present the development of a highly efficient and receptor-specific delivery platform for protein cargos by combining the receptor binding domain of Escherichia coli Shiga-like toxin and the translocation domain of Pseudomonas aeruginosa exotoxin A. We demonstrated the utility and efficiency of the delivery platform by showing a cytosolic delivery of diverse proteins both in vitro and in vivo in a receptor-specific manner. In particular, the delivery system was shown to be effective for targeting an intracellular protein and consequently suppressing the tumor growth in xenograft mice. The present platform can be widely used for intracellular delivery of diverse functional macromolecules with high efficiency in a receptor-specific manner. Biotechnol. Bioeng. 2016;113: 1639-1646. © 2016 Wiley Periodicals, Inc. PMID:26773973

  13. C-C chemokine receptor-7 mediated endocytosis of antibody cargoes into intact cells

    Directory of Open Access Journals (Sweden)

    FrançoisMarceau

    2013-09-01

    Full Text Available The C-C chemokine receptor-7 (CCR7 is a G protein coupled receptor that has a role in leukocyte homing, but that is also expressed in aggressive tumor cells. Preclinical research supports that CCR7 is a valid target in oncology. In view of the increasing availability of therapeutic monoclonal antibodies that carry cytotoxic cargoes, we studied the feasibility of forcing intact cells to internalize known monoclonal antibodies by exploiting the cycle of endocytosis and recycling triggered by the CCR7 agonist CCL19. Firstly, an anti-CCR7 antibody (CD197; clone 150503 labeled surface recombinant CCR7 expressed in intact HEK 293a cells and the fluorescent antibody was internalized following CCL19 treatment. Secondly, a recombinant myc-tagged CCL19 construction was exploited along the anti-myc monoclonal antibody 4A6. The myc-tagged ligand was produced as a conditioned medium of transfected HEK 293a cells that contained the equivalent of 430 ng/ml of immunoreactive CCL19 (average value, ELISA determination. CCL19-myc, but not authentic CCL19, carried the fluorophore-labeled antibody 4A6 into other recipient cells that expressed recombinant CCR7 (microscopy, cytofluorometry. The immune complexes were apparent in endosomal structures, colocalized well with the small GTPase Rab5 and progressed toward Rab7-positive endosomes. A dominant negative form of Rab5 (GDP-locked inhibited this endocytosis. Further, endosomes in CCL19-myc- or CCL19-stimulated cells were positive for β-arrestin2, but rarely for β-arrestin1. Following treatment with CCL19-myc and the 4A6 antibody, the melanoma cell line A375 that expresses endogenous CCR7 was specifically stained using a secondary peroxidase-conjugated antibody. Agonist-stimulated CCR7 can transport antibody-based cargoes, with possible therapeutic applications in oncology.

  14. Gestión de perfiles de cargos laborales basados en competencias

    Directory of Open Access Journals (Sweden)

    Franklin Sandoval

    2012-01-01

    Full Text Available El cambio vertiginoso que imponen los mercados, cada vez más globales y competitivos, necesariamente impacta en el desempeño de las organizaciones, donde el reto es mejorar su productividad; en este contexto surgen las competencias como un elemento base para la gestión del talento humano. La administración pública venezolana (APV no escapa de esta realidad; requiere mecanismos que apoyen una adecuada administración de las competencias laborales de sus empleados. Por esta razón, en este trabajo se desarrolla un modelo de gestión fundamentado en el conocimiento del dominio de las competencias laborales y perfiles de cargos, que estructura el proceso de gestión en tres actividades: 1 evaluación diagnóstica, 2 planeamiento y desarrollo de carrera, y 3 evaluación de desempeño. El enfoque metodológico utilizado en esta investigación fue proyectivo. Se obtuvo un modelo cíclico que promueve la revisión continua para la toma de decisiones y media entre los sub-sistemas de planificación estratégica y talento humano. Se concluye que una vez implementado el modelo, se podrán definir perfiles de cargos acordes a las expectativas de la organización, favoreciendo la productividad de sus empleados, propiciando rendimientos excelentes, así como la incorporación de mejoras y la concientización de los empleados orientada a la corresponsabilidad de su autodesarrollo

  15. Desigualdad de g??nero en la ocupaci??n de cargos directivos en el sistema sanitario p??blico en Espa??a

    OpenAIRE

    Delgado S??nchez, Ana; Saletti Cuesta, Lorena

    2010-01-01

    La medicina se feminiza pero existen obst??culos en la ocupaci??n de altos cargos. Objetivo=Describir la distribuci??n por sexo de cargos directivos del sistema sanitario p??blico (SSP) en Comunidades Aut??nomas (CCAA). Estudio descriptivo. Poblaci??n=Profesionales colegiados/as en medicina en 2008 por CCAA. Variables= sexo, cargos directivos de 1er y 2do nivel de consejer??as y servicios sanitarios en las 17 autonom??as del estado espa??ol. Fuentes=Instituto N...

  16. Advances in Autophagy Regulatory Mechanisms

    Directory of Open Access Journals (Sweden)

    Laura E. Gallagher

    2016-05-01

    Full Text Available Autophagy plays a critical role in cell metabolism by degrading and recycling internal components when challenged with limited nutrients. This fundamental and conserved mechanism is based on a membrane trafficking pathway in which nascent autophagosomes engulf cytoplasmic cargo to form vesicles that transport their content to the lysosome for degradation. Based on this simple scheme, autophagy modulates cellular metabolism and cytoplasmic quality control to influence an unexpectedly wide range of normal mammalian physiology and pathophysiology. In this review, we summarise recent advancements in three broad areas of autophagy regulation. We discuss current models on how autophagosomes are initiated from endogenous membranes. We detail how the uncoordinated 51-like kinase (ULK complex becomes activated downstream of mechanistic target of rapamycin complex 1 (MTORC1. Finally, we summarise the upstream signalling mechanisms that can sense amino acid availability leading to activation of MTORC1.

  17. Advances in Autophagy Regulatory Mechanisms.

    Science.gov (United States)

    Gallagher, Laura E; Williamson, Leon E; Chan, Edmond Y W

    2016-01-01

    Autophagy plays a critical role in cell metabolism by degrading and recycling internal components when challenged with limited nutrients. This fundamental and conserved mechanism is based on a membrane trafficking pathway in which nascent autophagosomes engulf cytoplasmic cargo to form vesicles that transport their content to the lysosome for degradation. Based on this simple scheme, autophagy modulates cellular metabolism and cytoplasmic quality control to influence an unexpectedly wide range of normal mammalian physiology and pathophysiology. In this review, we summarise recent advancements in three broad areas of autophagy regulation. We discuss current models on how autophagosomes are initiated from endogenous membranes. We detail how the uncoordinated 51-like kinase (ULK) complex becomes activated downstream of mechanistic target of rapamycin complex 1 (MTORC1). Finally, we summarise the upstream signalling mechanisms that can sense amino acid availability leading to activation of MTORC1. PMID:27187479

  18. Extraction Phase Simulation of Cargo Airdrop System%货台空投系统出舱过程研究

    Institute of Scientific and Technical Information of China (English)

    柯鹏; 杨春信; 杨雪松

    2006-01-01

    To study the characteristics of cargo extraction, the initial phase of airdrop process, a high fidelity and extendibility simulation model with uniform motion equations for all states during extraction is developed on the basis of dynamics methods and contact models between cargo and aircraft. Simulation results agree well with tests data. Cargo exit parameters, which contribute to cargo pitch after extraction, are studied. Simplified computation model of dimensionless exit time is developed and used to evaluate the relation between extraction phase and landing accuracy. Safe interval model is introduced to evaluate the safety of extraction process. Also,relations between initial parameters, including pull coefficient, aircraft pitch and CG coefficient, etc, and result parameters, including exit time, cargo safety, pitch, ete, are developed to help design of airdrop system. especially the selection of extraction parachute and cargo deployment.%研究了空投系统的出舱阶段中货台与地板接触模型,采用动力学分析方法建立了统一的出舱运动模型.根据试验设计条件开展系统仿真计算,结果与试验数据基本一致.探讨了货台离机过程对离机后俯仰姿态的影响.使用离机时间评价出舱过程对货台空投精确度的影响,得到了无量纲离机时间的简化算法以方便工程应用;引入了安全距离参数来评价出舱过程安全性,分析了牵引比、质心位置系数和空投时飞机姿态与离机时间和安全距离的关系,为空投系统中货台布局和牵引伞设计等提供了参考依据.

  19. El sistema de prestaciones por hijo a cargo en España

    Directory of Open Access Journals (Sweden)

    Obiol Francés, Sandra

    2006-04-01

    Full Text Available Child Benefit (prestaciones familiares por hijo a cargo is the only monetary, regular and direct allowance to contribute bringing up children in Spain. Therefore, the Family Allowance System might be seen as a privileged watchtower where it is possible to observe complex and dynamic relationships between the family, the State and the market. Moreover, it shows its influence on the wellbeing and on the dynamics of inclusion or exclusion. This paper analyzes the Spanish economic allowance addressed to children and its evolution since its last reform in 1990. The main goal is to contribute to our understanding of the compromise level acquired by the Spanish State for familycare. This study tries to draw where the limit between public and private areas is and which are the differences concerning the cost of chid bearing and education.

    Las prestaciones por hijo a cargo constituyen en España la única prestación de carácter monetario, periódico y directo que se otorga a nivel estatal para contribuir a los gastos generados por la crianza y educación de los hijos/as. Este hecho convierte al sistema de prestaciones en una privilegiada atalaya para observar las complejas y dinámicas relaciones que se dan entre familia, Estado y mercado en la canalización del bienestar y, por consiguiente, en las dinámicas de inclusión/exclusión de este mismo bienestar. En este artículo se analiza la evolución del sistema español de prestaciones económicas dirigidas a los hijos desde su última gran reforma en 1990, con la intención de que su estudio nos ayude a dilucidar qué grado de compromiso adquiere el Estado respecto a la atención a la familia, en qué lugar sitúa el límite entre el ámbito público y el ámbito privado, y dónde se incluye, entre esta diferenciación de esferas, la asunción del coste de la crianza y la educación de los hijos.

  20. Flight Schedule and the Circadian Clock Influence on Sleep Loss During Overnight Cargo Operations

    Science.gov (United States)

    Gander, Philippa H.; Gregory, Kevin B.; Rosekind, Mark R.; Shafto, Michael G. (Technical Monitor)

    1995-01-01

    Thirty-four flight crew members were monitored before, during, and after two 8-day overnight cargo duty patterns which involved multiple flights at night crossing no more than one time zone per 24 h. Rectal temperature, heart rate, and wrist activity were recorded every 2 min. Sleep quantity and quality, and nap timing, were noted in a logbook. To reduce the masking effects of physical activity on temperature, 0.28 C was added to each subject's raw temperature data whenever he reported being asleep. For both masked and unmasked data, daily temperature minima were estimated from the multiple complex demodulated waveform. The temperature minima did not show a progressive adaptation to night duty, which was interrupted by a night off after 5 nights on one trip pattern and after 3 nights on the other. On duty days, the average temperature minimum delayed by about 3 h, occurring near the end of the duty period. Daytime sleep episodes averaged 2.9 h shorter than nighttime sleep episodes, and were rated as lighter, less restorative, and poorer overall. Fifty-three percent of subjects slept more than once per 24 h while they were on night duty, compared to 17% when able to sleep at night. The total sleep per 24 h on duty days averaged 1.2 h less than pretrip. Twenty-nine percent of subjects lost more than 2 h of sleep per 24 h across the 8-day duty patterns. After night duty, subjects awoke around 1400 local time, even when they had slept 2-3 h less than a normal nocturnal sleep episode. Consequently, the duration of morning sleep episodes was correlated with the off-duty time (multiple r(sup 2)=0.44, F=37.23, p less than 0.0001). Anecdotally, crew members complained of being unable to sleep longer and not feeling well-rested. These wakeups were clustered 6 h after the temperature minimum, which suggests that they may have been a response to the circadian wakeup signal. Daytime layovers in which crew members were able to sleep again in the evening ended later (0200

  1. A flag-based algorithm and associated neutron interrogation system for the detection of explosives in sea-land cargo containers

    Science.gov (United States)

    Lehnert, A. L.; Kearfott, K. J.

    2015-07-01

    Recent efforts in the simulation of sea-land cargo containers in active neutron interrogation scenarios resulted in the identification of several flags indicating the presence of conventional explosives. These flags, defined by specific mathematical manipulations of the neutron and photon spectra, have been combined into a detection algorithm for screening cargo containers at international borders and seaports. The detection algorithm's steps include classifying the cargo type, identifying containers filled with explosives, triggering in the presence of concealed explosives, and minimizing the number of false positives due to cargo heterogeneity. The algorithm has been implemented in a system that includes both neutron and photon detectors. This system will take about 10 min to scan a container and cost approximately 1M to construct. Dose calculations resulted in estimates of less than 0.5 mSv for a person hidden in the container, and an operator annual dose of less than 0.9 mSv.

  2. A performance comparison of nuclear electric and nuclear thermal propulsion for Mars cargo missions across the 15-17 year synodic cycle

    Science.gov (United States)

    Sponaugle, Steven J.; Davis, Steven F.; Everett, Shonn F.

    1992-01-01

    This paper examines the effects of the Earth-Mars synodic cycle on Mars cargo missions. Cargo vehicles that use nuclear thermal propulsion are compared with those that use nuclear electric propulsion. It will be shown that for low energy class cargo missions, nuclear electric systems exhibit far less variation in peak performance over the synodic cycle than comparable nuclear thermal systems. Performance is measured by the amount of usable mass delivered to Mars, as well as the initial mass requirements in nuclear safe orbit. Nuclear electric propulsion systems also have significantly longer injection window opportunities for a given 26 month synodic period, resulting in much greater mission design flexibility. Injection window opportunities over a 20 year period from 2010 to 2030 are examined. This covers a complete synodic cycle and shows its effects on performance for Mars cargo missions.

  3. Light or Heat? The Origin of Cargo Release from Nanoimpeller Particles Containing Upconversion Nanocrystals under IR Irradiation.

    Science.gov (United States)

    Dong, Juyao; Zink, Jeffrey I

    2015-09-01

    Nanoimpellers are mesoporous silica nanoparticles that contain azobenzene derivatives bonded inside the pores and rely on the continuous photoisomerization of multiple azobenzenes to release cargo under near UV irradiation. A recent study employs upconversion nanocrystal embedded particles to replace UV light with IR light to stimulate nanoimpellers. However, the photothermal effect of IR irradiation and its likely contribution to the observed release behavior are not examined. It is found that, in the absence of upconversion nanocrystals, the azobenzene co-condensed silica particles still respond to 980 nm illumination, which increases the nanoparticle temperature by 25 °C in 15 min, experimentally measured by an encapsulated nanothermometer. After suppressing the heating, the IR irradiation does not initiate the release, indicating that optical heating, not upconverted light, is responsible for the triggered cargo release. The results are explained by numerical analyses. PMID:26034008

  4. A biomimetic DNA-based channel for the ligand-controlled transport of charged molecular cargo across a biological membrane

    Science.gov (United States)

    Burns, Jonathan R.; Seifert, Astrid; Fertig, Niels; Howorka, Stefan

    2016-02-01

    Biological ion channels are molecular gatekeepers that control transport across cell membranes. Recreating the functional principle of such systems and extending it beyond physiological ionic cargo is both scientifically exciting and technologically relevant to sensing or drug release. However, fabricating synthetic channels with a predictable structure remains a significant challenge. Here, we use DNA as a building material to create an atomistically determined molecular valve that can control when and which cargo is transported across a bilayer. The valve, which is made from seven concatenated DNA strands, can bind a specific ligand and, in response, undergo a nanomechanical change to open up the membrane-spanning channel. It is also able to distinguish with high selectivity the transport of small organic molecules that differ by the presence of a positively or negatively charged group. The DNA device could be used for controlled drug release and the building of synthetic cell-like or logic ionic networks.

  5. 46 CFR 32.60-40 - Construction and testing of cargo tanks and bulkheads-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Construction and testing of cargo tanks and bulkheads-TB/ALL. 32.60-40 Section 32.60-40 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL EQUIPMENT, MACHINERY, AND HULL REQUIREMENTS Hull Requirements for Tank Vessels Constructed On or After July 1, 1951 § 32.60-40 Construction...

  6. Monte-Carlo Simulations of Radiation-Induced Activation in a Fast-Neutron and Gamma- Based Cargo Inspection System

    OpenAIRE

    Bromberger, B.; Bar, D.; Brandis, M.; Dangendorf, V.; Goldberg, M B; Kaufmann, F.; Mor, I.; Nolte, R.; SCHMIEDEL M.; Tittelmeier, K.; Vartsky, D.; H. Wershofen

    2012-01-01

    An air cargo inspection system combining two nuclear reaction based techniques, namely Fast-Neutron Resonance Radiography and Dual-Discrete-Energy Gamma Radiography is currently being developed. This system is expected to allow detection of standard and improvised explosives as well as special nuclear materials. An important aspect for the applicability of nuclear techniques in an airport inspection facility is the inventory and lifetimes of radioactive isotopes produced by the neutron and ga...

  7. Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins

    OpenAIRE

    del Castillo, F. J.; Cohen-Salmon, M; Charollais, A.; Caille, D.; Lampe, P. D.; Chavrier, P; Meda, P.; Petit, C

    2009-01-01

    Targeting of numerous transmembrane proteins to the cell surface is thought to depend on their recognition by cargo receptors that interact with the adaptor machinery for anterograde traffic at the distal end of the Golgi complex. We report here on consortin, a novel integral membrane protein that is predicted to be intrinsically disordered, i.e. that contains large segments whose native state is unstructured. We identified consortin as a binding partner of connexins, the building blocks of g...

  8. Knowledge management for improving business processes: an analysis of the transport management process for indivisible exceptional cargo

    OpenAIRE

    André Cristiano Silva Melo; Maria Aparecida Cavalcanti Netto; Virgílio José Martins Ferreira Filho; Elton Fernandes

    2010-01-01

    This paper presents an organizational analysis methodology aimed at knowledge capitalization with a view to improving business processes. Based on a real problem in a large electric sector firm, this methodology is applied to managing the transport of indivisible exceptional cargo (IEC). In the firm in question, intellectual capital is a critical asset for service performance and is fundamental for achieving business excellence. Applied to the firm's transport management process, the approach...

  9. Models for microtubule cargo transport coupling the Langevin equation to stochastic stepping motor dynamics: Caring about fluctuations.

    Science.gov (United States)

    Bouzat, Sebastián

    2016-01-01

    One-dimensional models coupling a Langevin equation for the cargo position to stochastic stepping dynamics for the motors constitute a relevant framework for analyzing multiple-motor microtubule transport. In this work we explore the consistence of these models focusing on the effects of the thermal noise. We study how to define consistent stepping and detachment rates for the motors as functions of the local forces acting on them in such a way that the cargo velocity and run-time match previously specified functions of the external load, which are set on the base of experimental results. We show that due to the influence of the thermal fluctuations this is not a trivial problem, even for the single-motor case. As a solution, we propose a motor stepping dynamics which considers memory on the motor force. This model leads to better results for single-motor transport than the approaches previously considered in the literature. Moreover, it gives a much better prediction for the stall force of the two-motor case, highly compatible with the experimental findings. We also analyze the fast fluctuations of the cargo position and the influence of the viscosity, comparing the proposed model to the standard one, and we show how the differences on the single-motor dynamics propagate to the multiple motor situations. Finally, we find that the one-dimensional character of the models impede an appropriate description of the fast fluctuations of the cargo position at small loads. We show how this problem can be solved by considering two-dimensional models. PMID:26871095

  10. Four legs or two: up front with you--preventing tragedies by keeping children and pets out of the cargo areas of pickup trucks.

    Science.gov (United States)

    McHugh, E J

    1999-08-01

    Dogs and children riding in the cargo area of pickup trucks are common sights on our nation's highways, country roads, and small town streets. This scene brings to mind idyllic childhoods filled with summer days spent bouncing down roads in the back of grandpa's pickup truck, wind catching the laughter from children's throats. For hundreds of families each year, this activity is the cause of heartache, traumatic injuries, and senseless deaths. More than 200 people die each year, half of whom are younger than 18 years of age, as a direct result of falls from cargo areas of pickup trucks. Injuries and deaths resulting from riding in cargo areas of pickup trucks are not limited to humans. In a recent survey, 71% of veterinarians reported treating dogs for injuries sustained by falling or jumping from cargo areas of pickup trucks. This percentage does not take into account countless dogs that were killed and not treated. Injuries and deaths resulting from riding in cargo areas of pickup trucks are preventable. Whereas it may take millions of dollars and many years to cure diseases such as cancer, heart disease, and AIDS, pickup truck-associated deaths can be prevented simply by educating the public to not allow children and pets to ride in cargo areas. The primary objective of "Four legs or two: Up front with you" is to educate the public on the risks involved with travel in the cargo areas of pickup trucks. Making people aware that children and pets are not cargo is the first step toward decreasing senseless injuries and deaths. PMID:10434968

  11. Los sistemas de cargos en la Cuenca de México: una primera aproximación a su trasfondo histórico

    OpenAIRE

    ANDRÉS MEDINA

    1995-01-01

    This article introduces an interesting approach regarding the historical environment of the discussion of the cargo systems in Mexico. The latter is exposed as a framework in order to establish, from a historical perspective, some specific elements of cargo systems, the cosmovision and the sacred geography of the Mexican valley, which suggests to be undertaken not only regarding the economical, ecological and political, but also, what is related to religious beliefs and ritual practices.

  12. A Proposal for High-resolution X-ray Imaging of Intermodal Cargo Containers for Fissionable Materials

    Energy Technology Data Exchange (ETDEWEB)

    Quiter, B J; Prussin, S G; Pohl, B; Hall, J; Trebes, J; Stone, G; Descalle, M

    2007-07-05

    The sensitivity for identification of high-Z objects in elemental form in the massive cargo of intermodal containers with continuous bremsstrahlung radiation depends critically on discriminating the weak signal from uncollided photons from the very intense flux of scattered radiations that penetrate the cargo. We propose that this might be accomplished by rejection of detected events with E {le} 2-3 MeV that contain the majority of multiply-scattered photons along with a correction for single-scattered photons at higher energies. Monte Carlo simulations of radiographs with a 9-MeV bremsstrahlung spectrum demonstrate that rejection of detected events with E {le} 3 MeV removes the majority of signals from scattered photons emerging through cargo with Z {le} 30 and areal densities of at least 145 g cm{sup -2}. With analytical estimates of the single-scattered intensity at higher energies, accurate estimates of linear attenuation coefficients for shielded and unshielded uranium spheres with masses as small as 0.08 kg are found. The estimated maximum dose is generally so low that reasonable order tomography of interesting portions of a container should be possible.

  13. National Security Science and Technology Initiative: Air Cargo Screening, Final Report for CRADA Number NFE-07-01081

    Energy Technology Data Exchange (ETDEWEB)

    Bingham, Philip [ORNL; Bush, John [Battelle Memorial Institute; Bowerman, Biays [Brookhaven National Laboratory; Cespedes, Ernesto [Idaho National Laboratory; White, Timothy [Pacific Northwest National Laboratory

    2004-12-01

    The non-intrusive inspection (NII) of consolidated air cargo carried on commercial passenger aircraft continues to be a technically challenging, high-priority requirement of the Department of Homeland Security’s Science and Technology Directorate (DHS S&T), the Transportation Security Agency and the Federal Aviation Administration. The goal of deploying a screening system that can reliably and cost-effectively detect explosive threats in consolidated cargo without adversely affecting the flow of commerce will require significant technical advances that will take years to develop. To address this critical National Security need, the Battelle Memorial Institute (Battelle), under a Cooperative Research and Development Agreement (CRADA) with four of its associated US Department of Energy (DOE) National Laboratories (Oak Ridge, Pacific Northwest, Idaho, and Brookhaven), conducted a research and development initiative focused on identifying, evaluating, and integrating technologies for screening consolidated air cargo for the presence of explosive threats. Battelle invested $8.5M of internal research and development funds during fiscal years 2007 through 2009.

  14. Cargo Aircraft Conceptual Design Optimisation Using a Flexible Computer-Based Scaling Approach

    Directory of Open Access Journals (Sweden)

    F. Schieck

    2001-01-01

    Full Text Available In the early design stages of a new aircraft, there is a strong need to broaden the knowledge base of the evolving aircraft project, allowing a profound analysis of the solution concepts and of the design driving requirements. The methodology presented in this paper provides a tool for increasing and improving in an exemplary manner the necessary information on cargo aircraft. By exchanging or adapting a few particular modules of the entire program system, the tool is applicable to a range scale of different aircraft types. In an extended requirement model, performance requirements are represented along with other operational requirements. An aircraft model is introduced in sufficient detail for conceptual design considerations. The computer-aided scaling methodology is explained, which, controlled by an optimisation module, automatically resizes the aircraft model until it optimally satisfies the requirements in terms of a selectable figure of merit. Typical results obtained at the end of the scaling are discussed together with knowledge gained during the process, and an example is given.

  15. Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model

    Science.gov (United States)

    Villasante, Aranzazu; Marturano-Kruik, Alessandro; Ambati, Srikanth R.; Liu, Zen; Godier-Furnemont, Amandine; Parsa, Hesam; Lee, Benjamin W.; Moore, Malcolm A.S.; Vunjak-Novakovic, Gordana

    2016-01-01

    There is a growing interest in the pivotal role of exosomes in cancer and in their use as biomarkers. However, despite the importance of the microenvironment for cancer initiation and progression, monolayer cultures of tumor cells still represent the main in vitro source of exosomes. As a result, their environmental regulation remains largely unknown. Here, we report a three-dimensional tumor model for studying exosomes, using Ewing's sarcoma type 1 as a clinically relevant example. The bioengineered model was designed based on the hypothesis that the 3-dimensionality, composition and stiffness of the tumor matrix are the critical determinants of the size and cargo of exosomes released by the cancer cells. We analyzed the effects of the tumor microenvironment on exosomes, and the effects of exosomes on the non-cancer cells from the bone niche. Exosomes from the tissue-engineered tumor had similar size distribution as those in the patients' plasma, and were markedly smaller than those in monolayer cultures. Bioengineered tumors and the patients' plasma contained high levels of the Polycomb histone methyltransferase EZH2 mRNA relatively to their monolayer counterparts. Notably, EZH2 mRNA, a potential tumor biomarker detectable in blood plasma, could be transferred to the surrounding mesenchymal stem cells. This study provides the first evidence that an in vitro culture environment can recapitulate some properties of tumor exosomes. PMID:27279906

  16. The EURITRACK project: development of a tagged neutron inspection system for cargo containers

    Science.gov (United States)

    Perot, Bertrand; Perret, Gregory; Mariani, Alain; Ma, Jean-Luc; Szabo, Jean-Louis; Mercier, Emmanuel; Sannie, Guillaume; Viesti, Giuseppe; Nebbia, Giancarlo; Pesente, Silvia; Lunardon, Marcello; Formisano, Paola; Moretto, Sandra; Fabris, Daniela; Zenoni, Aldo; Bonomi, Germano; Donzella, Antonietta; Fontana, Andrea; Boghen, Gaia; Valkovic, Vladivoj; Sudac, Darovin; Moszynski, Marek; Batsch, Tadeusz; Gierlik, Michal; Wolski, Dariusz; Klamra, Wlodzimierz; Isaksson, Patrick; Le Tourneur, Philippe; Lhuissier, Miguel; Colonna, Annamaria; Tintori, Carlo; Peerani, Paolo; Sequeira, Vitor; Salvato, Martino

    2006-05-01

    The EURopean Illicit TRAfficing Countermeasures Kit project is part of the 6th European Union Framework Program, and aims at developing a neutron inspection system for detecting threat materials (explosives, drugs, etc.) in cargo containers. Neutron interaction in the container produces specific gamma-rays used to determine the chemical composition of the inspected material. An associated particle sealed tube neutron generator is developed to allow precise location of the interaction point by direction and time-of-flight measurements of the neutrons tagged by alpha-particles. The EURITRACK project consists in developing: a transportable deuterium-tritium neutron generator including a position sensitive alpha detector (8×8 matrix of YAP:Ce crystals coupled to a multi-anode photomultiplier), fast neutron and gamma-ray detectors, front-end electronics to perform coincidence and spectroscopic measurements, and an integrated software which manages neutron generator and detectors positioning, data acquisition and analysis. Hardware components have been developed and tested by the consortium partners. Current status of this work and provisional performances of the system assessed by Monte Carlo calculations are presented.

  17. Structural response of cargo containment systems in LNG carriers under ice loads

    International Nuclear Information System (INIS)

    Gas exploration has been extended into the Arctic region such as in the Russian Arctic area, because of the increasing demand for energy resources. As a result, shipping in ice-covered seas is also increasing. Many technical issues are involved in ensuring the safety of liquefied natural gas (LNG) ships during the transportation. This paper discussed an investigation of ship-ice interaction scenarios for possible operation routes in Arctic areas. Six scenarios were selected to study the structural response of cargo containment systems (CCS) in both membrane and spherical types of LNG ships. For selected ship-ice interaction scenarios, ice loads and loading areas in the hull structure were determined based on the energy theory. The configurations of LNG carriers were discussed and illustrated. The paper also outlined the assessment criteria and structure analysis procedures. It was concluded that the strength of the CCS of membrane-type LNG carrier and the strength of the skirt structure of spherical-type LNG carrier were strong enough under the design ice loads. 13 refs., 9 tabs., 18 figs

  18. Logistics worldwide for the whole nuclear cycle. Nuclear Cargo + Service GmbH

    International Nuclear Information System (INIS)

    Nuclear Cargo + Service GmbH (NCS) offers logistics worldwide for the whole nuclear cycle to fuel manufacturers, reactor operators, and waste management companies. Transport equipment and interim stores are part of the company's facilities. The firm's Front End unit conducts transnational transports of yellow cake, uranium hexafluoride, and enriched uranium and uranium from reprocessing. In the Back End unit, the company performs worldwide transports of spent fuel elements of research reactors. Another key area of activity is shipment of radioactive waste ranging from low-level waste to HAW (highly active waste generating heat). In its Component Disposal area, NCS offers a variety of transport services up to and including large components, e.g. reactor pressure vessels, both on the road and by rail and ship. NCS operates storage halls of its own for storing radioactive materials including radioactive waste. The technical equipment of NCS comprises a large pool of road and rail transport vehicles as well as a great number of vessels for radioactive materials. (orig.)

  19. Monte Carlo parametric studies of neutron interrogation with the Associated Particle Technique for cargo container inspections

    Science.gov (United States)

    Deyglun, Clément; Carasco, Cédric; Pérot, Bertrand

    2014-06-01

    The detection of Special Nuclear Materials (SNM) by neutron interrogation is extensively studied by Monte Carlo simulation at the Nuclear Measurement Laboratory of CEA Cadarache (French Alternative Energies and Atomic Energy Commission). The active inspection system is based on the Associated Particle Technique (APT). Fissions induced by tagged neutrons (i.e. correlated to an alpha particle in the DT neutron generator) in SNM produce high multiplicity coincidences which are detected with fast plastic scintillators. At least three particles are detected in a short time window following the alpha detection, whereas nonnuclear materials mainly produce single events, or pairs due to (n,2n) and (n,n'γ) reactions. To study the performances of an industrial cargo container inspection system, Monte Carlo simulations are performed with the MCNP-PoliMi transport code, which records for each neutron history the relevant information: reaction types, position and time of interactions, energy deposits, secondary particles, etc. The output files are post-processed with a specific tool developed with ROOT data analysis software. Particles not correlated with an alpha particle (random background), counting statistics, and time-energy resolutions of the data acquisition system are taken into account in the numerical model. Various matrix compositions, suspicious items, SNM shielding and positions inside the container, are simulated to assess the performances and limitations of an industrial system.

  20. Proteomic analysis of exosomal cargo: the challenge of high purity vesicle isolation.

    Science.gov (United States)

    Abramowicz, Agata; Widlak, Piotr; Pietrowska, Monika

    2016-04-26

    The re-discovery of exosomes as intercellular messengers with high potential for diagnostic and therapeutic utility has led to them becoming a popular topic of research in recent years. One of the essential research areas in this field is the characterization of exosomal cargo, which includes numerous non-randomly packed proteins and nucleic acids. Unexpectedly, a very challenging aspect of exploration of extracellular vesicles has turned out to be their effective and selective isolation. The plurality of developed protocols leads to qualitative and quantitative variability in terms of the obtained exosomes, which significantly affects the results of downstream analyses and makes them difficult to compare, reproduce and interpret between research groups. Currently, there is a general consensus among the exosome-oriented community concerning the urgent need for the optimization and standardization of methods employed for the purification of these vesicles. Hence, we review here several strategies for exosome preparation including ultracentrifugation, chemical precipitation, affinity capturing and filtration techniques. The advantages and disadvantages of different approaches are discussed with special emphasis being placed on their adequacy for proteomics applications, which are particularly sensitive to sample quality. We conclude that certain methods, exemplified by ultracentrifugation combined with iodixanol density gradient centrifugation or gel filtration, although labor-intensive, provide superior quality exosome preparations suitable for reliable analysis by mass spectrometry. PMID:27030573

  1. AN INVESTIGATION OF THE BREAKING DYNAMICS OF A VEHICLE WHICH CARRIES LIQUID CARGO

    Directory of Open Access Journals (Sweden)

    Murat REİS

    2006-03-01

    Full Text Available In this study, the effects of fluid motion in external fuel tanks on the dynamics of breaking system are investigated. The unconstrained motion of fluid also called sloshing causes high dynamic loads especially in highly accelerated motion of massive bodies. These dynamic loads affect the dynamic behavior of truck, and, inversely, the reaction of truck affects the fluid motion. The interaction between fluid and truck affects the whole system's dynamics. In this study, the breaking performance of truck carrying fluid cargo at various ratios of plenitude is investigated using simulation techniques via Msc.Dytran and the swinging pendulum method. The kinetic coefficient of friction is assumed to be constant during the motion. The effect of fluid motion on the system's dynamic has been compared for various plenitude ratios, and the efficiency of pendulum method is compared. The feasibility of pendulum method is also discussed. As a result of comparison of numerical simulations, it has been concluded that the effect of fluid motion on the truck's breakage performance in the case of % 50 plentitude ratio is higher than that of the ratios of plenitude greater than % 50. On the other hand, it has been observed that the pendulum method is useful for the plenitude ratios less than % 50 and for accelerations of a ? (1/ 2g .

  2. State of the Art and Development Trends of the Digital Radiography Systems for Cargo Inspection

    Science.gov (United States)

    Udod, V.; Van, J.; Osipov, S.; Chakhlov, S.; Temnik, A.

    2016-01-01

    Increasing requirements for technical parameters of inspection digital radiography systems are caused by increasing incidences of terrorism, drug trafficking and explosives via variety of transport. These requirements have determined research for new technical solutions that enable to ensure the safety of passengers and cargos in real-time. The main efforts in the analyzed method of testing are aimed at the creation of new and modernization of operated now systems of digital radiography as a whole and their main components and elements in particular. The number of these main components and elements includes sources of X-ray recording systems and transformation of radiometric information as well as algorithms and software that implements these algorithms for processing, visualization and results interpretation of inspection. Recent developments of X-ray units and betatrons used for inspection of small- and large-sized objects that are made from different materials are deserve special attention. The most effective X-ray detectors are a line and a radiometric detector matrix based on various scintillators. The most promising methods among the algorithms of material identification of testing objects are dual-energy methods. The article describes various models of digital radiography systems applied in Russia and abroad to inspection of baggage, containers, vehicles and large trucks.

  3. Standards and conventions for the Worldwide Port System (WPS) regional Integrated Cargo Database (ICDB)

    Energy Technology Data Exchange (ETDEWEB)

    Loftis, J.P.; Truett, L.F.; Shipe, P.C. [Oak Ridge National Lab., TN (United States); Faby, E.Z.; Fluker, J.; Grubb, J.W.; Hancock, B.R. [Univ. of Tennessee, Knoxville, TN (United States); Ferguson, R.A. [Science Applications International Corp., Oak Ridge, TN (United States)

    1995-02-28

    This document, prepared for the Worldwide Port System (WPS) Regional Integrated Cargo Database (ICDB), provides standards and conventions for the screens developed using ORACLE`s SQL*Menu, SQL*Forms, and SQL*Reportwriter; for the ORACLE keys; and for commenting ORACLE code. It also covers standards for database system transfers. The results of adherence to these standards and conventions by all developers at both geographically separated development sites, Oak Ridge National Laboratory (ORNL) and The Military Traffic Management Command`s Eastern Area (EA), will be a consistent appearance of ICDB to users, code that is easily maintained, and a system that will be quicker to develop and integrate. This final report of the Standards and Conventions contains general guidelines to be followed for the development of the ICDB user interface screens. Though additional ICDB user interface screens are being developed both at ORNL and EA, and existing screens may have fields added to or deleted from them, the standards and conventions presented in this document should remain unchanged.

  4. ExoCarta: A Web-Based Compendium of Exosomal Cargo.

    Science.gov (United States)

    Keerthikumar, Shivakumar; Chisanga, David; Ariyaratne, Dinuka; Al Saffar, Haidar; Anand, Sushma; Zhao, Kening; Samuel, Monisha; Pathan, Mohashin; Jois, Markandeya; Chilamkurti, Naveen; Gangoda, Lahiru; Mathivanan, Suresh

    2016-02-22

    Exosomes are membranous vesicles that are released by a variety of cells into the extracellular microenvironment and are implicated in intercellular communication. As exosomes contain RNA, proteins and lipids, there is a significant interest in characterizing the molecular cargo of exosomes. Here, we describe ExoCarta (http://www.exocarta.org), a manually curated Web-based compendium of exosomal proteins, RNAs and lipids. Since its inception, the database has been highly accessed (>54,000 visitors from 135 countries). The current version of ExoCarta hosts 41,860 proteins, >7540 RNA and 1116 lipid molecules from more than 286 exosomal studies annotated with International Society for Extracellular Vesicles minimal experimental requirements for definition of extracellular vesicles. Besides, ExoCarta features dynamic protein-protein interaction networks and biological pathways of exosomal proteins. Users can download most often identified exosomal proteins based on the number of studies. The downloaded files can further be imported directly into FunRich (http://www.funrich.org) tool for additional functional enrichment and interaction network analysis. PMID:26434508

  5. Book Review: Radiation protection and measurement issues related to cargo scanning with accelerator-produced high-energy X rays, NCRP Commentary No. 20

    International Nuclear Information System (INIS)

    Having spent roughly the first third of his health physics career on the Norfolk, VA waterfront area, the reviewer was excited to see the NCRP Commentary 20, 'Radiation Protection and Measurements Issues Related to Cargo Scanning with Accelerator Technology'. It signals the advent of the Cargo Advanced Automated Radiography System (CAARS). The waterfront is a border that challenges physical security programs and technology. As Commentary 20 provides in the introduction, waterfront cargo terminals and land border crossings together represent over 300 ports of entry in the USA. Every year, the USA receives over 10 million cargo containers from commercial shipping and a roughly equal amount from land border crossings. While rapidly processing containerized cargo, CAARS will be able to detect small quantities of high atomic number radioactive materials and dense shielding materials used for radioactive gamma ray sources and even illicit human cargo - important concerns for homeland security. It will also be able to detect other contraband such as explosives, weapons and drugs. Section 1 of the Commentary presents an executive summary with NCRP's radiation dose management recommendations and related operational recommendations for effective implementation of CAARS technology in the current regulatory environment.

  6. Book Review: Radiation protection and measurement issues related to cargo scanning with accelerator-produced high-energy X rays, NCRP Commentary No. 20

    Energy Technology Data Exchange (ETDEWEB)

    Robert May

    2008-11-01

    Having spent roughly the first third of his health physics career on the Norfolk, VA waterfront area, the reviewer was excited to see the NCRP Commentary 20, 'Radiation Protection and Measurements Issues Related to Cargo Scanning with Accelerator Technology'. It signals the advent of the Cargo Advanced Automated Radiography System (CAARS). The waterfront is a border that challenges physical security programs and technology. As Commentary 20 provides in the introduction, waterfront cargo terminals and land border crossings together represent over 300 ports of entry in the USA. Every year, the USA receives over 10 million cargo containers from commercial shipping and a roughly equal amount from land border crossings. While rapidly processing containerized cargo, CAARS will be able to detect small quantities of high atomic number radioactive materials and dense shielding materials used for radioactive gamma ray sources and even illicit human cargo - important concerns for homeland security. It will also be able to detect other contraband such as explosives, weapons and drugs. Section 1 of the Commentary presents an executive summary with NCRP's radiation dose management recommendations and related operational recommendations for effective implementation of CAARS technology in the current regulatory environment.

  7. Formation of the mechanism of interaction of the motor transportation enterprise with subjects the market of the international freight traffics

    Directory of Open Access Journals (Sweden)

    A.M.Ponomaryov

    2011-12-01

    Full Text Available Takes up a question of formation of the mechanism of interaction of the motor transportation enterprise (MC in the market of the international cargo automobile transportations (ICAT with subjects of the market. Given the characteristic of the basic subjects which operate in market ICAT. The innovation model card clientenoughtable MC in market of ICAT is offered.

  8. Solid peptide nanoparticles--structural characterization and quantification of cargo encapsulation.

    Science.gov (United States)

    Dittrich, Christian; Meier, Wolfgang

    2010-12-01

    CD3ac, an uncharged and strongly hydrophobic 10 amino acid peptide (Ac-LK(Ac)-LK(Ac)-LK(Ac)-LW-DL-LW-DL-LW-DL-LW-NH2) was synthesized and purified. The peptide readily dissolves in ethanol and--upon solvent exchange to water--assembles into solid spherical particles with diameters of around 500 nm and low size-polydispersity. CD3ac self-assembles in a convenient one-step-process in the absence of a templating two-phase solvent system or any other templating agents. Circular dichroism reveals a gramicidin-like secondary structure, which can be attributed to the presence of D-leucine, whereas LCD3ac, a peptide of identical constitution yet composed entirely of L-amino acids precipitates amorphously. The unacetylated derivative of LCD3ac (LCD3) displays α-helical character in circular dichroism. During the process of bead formation, CD3ac can take up and enrich water-soluble and--insoluble cargo compounds, which is exemplified by the encapsulation of rose bengal (RB) and 5-carboxy-fluorescein (CF), two xanthene derivatives. We confirmed their presence in CD3ac beads by confocal fluorescence microscopy and quantified the encapsulation efficiency by absorption measurements of dissolved RB-containing peptide bead suspensions. Loaded CD3ac beads consist of up to 40 mol-% RB, which corresponds to a logarithmic partition coefficient of 2.95. To the best of our knowledge CD3ac is the first peptide synthesized by Fmoc chemistry which forms solid particles in the nano- and micrometer size range and holds promise for drug delivery applications. PMID:21166104

  9. Multimotor Driven Cargos: From Single Motor under Load to the Role of Motor-Motor Coupling.

    Science.gov (United States)

    Peker, Itay; Granek, Rony

    2016-07-01

    Motor proteins constitute an essential part of the cellular machinery. They have been the subject of intensive studies in the past two decades. Yet, when several motors simultaneously carry a single cargo, the effect of motor-motor coupling, such as mutual stalling and jamming, remains unclear. We commence by constructing a general model for single motor motion, which is a product of a derived load-dependent expression and a phenomenological motor specific function. Forming the latter according to recent single molecule measurements for a given load, the model correctly predicts the motor full step-size distribution for all other measured loads. We then use our proposed model to predict transport properties of multimotor complexes, with particular attention to 1-dimensional constructs with variable flexibility, motor density, and number of motors: (i) a chain of motors connected by springs, a recently studied construction of a pair, and (ii) an array of motors all connected by identical springs to a stiff rod, which is essentially a mirror image of standard gliding motility assays. In both systems, and for any number of carrying motors, we find that, while low flexibility results in a strongly damped velocity, increased flexibility renders an almost single motor velocity. Comparing our model based simulations to recent gliding assays we find remarkable qualitative agreement. We also demonstrate consistency with other multimotor motility assays. In all cases, the characteristic spring constant, that controls the crossover behavior between high and low velocity regimes, is found to be the stalling force divided by the mean step size. We conjecture that this characteristic spring constant can serve as a tool for engineering multimotor complexes. PMID:27044876

  10. A New Heavy-Lift Capability for Space Exploration: NASA's Ares V Cargo Launch Vehicle

    Science.gov (United States)

    Sumrall, John P.; McArthur, J. Craig

    2007-01-01

    The National Aeronautics and Space Administration (NASA) is developing new launch systems and preparing to retire the Space Shuttle by 2010, as directed in the United States (U.S.) Vision for Space Exploration. The Ares I Crew Launch Vehicle (CLV) and the Ares V heavy-lift Cargo Launch Vehicle (CaLV) systems will build upon proven, reliable hardware derived from the Apollo-Saturn and Space Shuttle programs to deliver safe, reliable, affordable space transportation solutions. This approach leverages existing aerospace talent and a unique infrastructure, as well as legacy knowledge gained from nearly 50 years' experience developing space hardware. Early next decade, the Ares I will launch the new Orion Crew Exploration Vehicle (CEV) to the International Space Station (ISS) or to low-Earth orbit for trips to the Moon and, ultimately, Mars. Late next decade, the Ares V's Earth Departure Stage will carry larger payloads such as the lunar lander into orbit, and the Crew Exploration Vehicle will dock with it for missions to the Moon, where astronauts will explore new territories and conduct science and technology experiments. Both Ares I and Ares V are being designed to support longer future trips to Mars. The Exploration Launch Projects Office is designing, developing, testing, and evaluating both launch vehicle systems in partnership with other NASA Centers, Government agencies, and industry contractors. This paper provides top-level information regarding the genesis and evolution of the baseline configuration for the Ares V heavy-lift system. It also discusses riskbased, management strategies, such as building on powerful hardware and promoting common features between the Ares I and Ares V systems to reduce technical, schedule, and cost risks, as well as development and operations costs. Finally, it summarizes several notable accomplishments since October 2005, when the Exploration Launch Projects effort officially kicked off, and looks ahead at work planned for 2007

  11. Monte-Carlo simulations of neutron-induced activation in a Fast-Neutron and Gamma-Based Cargo Inspection System

    Science.gov (United States)

    Bromberger, B.; Bar, D.; Brandis, M.; Dangendorf, V.; Goldberg, M. B.; Kaufmann, F.; Mor, I.; Nolte, R.; Schmiedel, M.; Tittelmeier, K.; Vartsky, D.; Wershofen, H.

    2012-03-01

    An air cargo inspection system combining two nuclear reaction based techniques, namely Fast-Neutron Resonance Radiography and Dual-Discrete-Energy Gamma Radiography is currently being developed. This system is expected to allow detection of standard and improvised explosives as well as special nuclear materials. An important aspect for the applicability of nuclear techniques in an airport inspection facility is the inventory and lifetimes of radioactive isotopes produced by the neutron radiation inside the cargo, as well as the dose delivered by these isotopes to people in contact with the cargo during and following the interrogation procedure. Using MCNPX and CINDER90 we have calculated the activation levels for several typical inspection scenarios. One example is the activation of various metal samples embedded in a cotton-filled container. To validate the simulation results, a benchmark experiment was performed, in which metal samples were activated by fast-neutrons in a water-filled glass jar. The induced activity was determined by analyzing the gamma spectra. Based on the calculated radioactive inventory in the container, the dose levels due to the induced gamma radiation were calculated at several distances from the container and in relevant time windows after the irradiation, in order to evaluate the radiation exposure of the cargo handling staff, air crew and passengers during flight. The possibility of remanent long-lived radioactive inventory after cargo is delivered to the client is also of concern and was evaluated.

  12. A flag-based algorithm and associated neutron interrogation system for the detection of explosives in sea–land cargo containers

    International Nuclear Information System (INIS)

    Recent efforts in the simulation of sea–land cargo containers in active neutron interrogation scenarios resulted in the identification of several flags indicating the presence of conventional explosives. These flags, defined by specific mathematical manipulations of the neutron and photon spectra, have been combined into a detection algorithm for screening cargo containers at international borders and seaports. The detection algorithm's steps include classifying the cargo type, identifying containers filled with explosives, triggering in the presence of concealed explosives, and minimizing the number of false positives due to cargo heterogeneity. The algorithm has been implemented in a system that includes both neutron and photon detectors. This system will take about 10 min to scan a container and cost approximately $1M to construct. Dose calculations resulted in estimates of less than 0.5 mSv for a person hidden in the container, and an operator annual dose of less than 0.9 mSv. - Highlights: • Monte Carlo model of explosives screening in cargo containers using fast neutrons. • Monte Carlo model also explores neutron detector response. • Development of an explosives detection algorithm using active neutron interrogation. • Implementation of the algorithm, including equipment, infrastructure, cost and dose. • System will cost ~1M USD and an entire container may be scanned in ~10 min

  13. La temporalidad de los cargos públicos en la Roma republicana: motivo de reflexión

    OpenAIRE

    Gil García, Olga

    2012-01-01

    La duración limitada de las magistraturas en la Roma republicana y su posterior decadencia con la prorrogatio imperii, nos hace reflexionar sobre la relevancia de la temporalidad de los cargos públicos como garantía de un sistema democrático. Utilizando el ejemplo romano, comparamos lo que sucede desde la entrada en vigor de nuestra Constitución de 1978 hasta hoy, y observamos con preocupación, como la durabilidad en los mandatos de los principales órganos constitucionales se está relajando, ...

  14. La temporalidad de los cargos públicos en la Roma republicana: motivo de reflexión

    OpenAIRE

    Gil García, Olga

    2012-01-01

    La duración limitada de las magistraturas en la Roma republicana y su posterior decadencia con la prorrogatio imperii, nos hace reflexionar sobre la relevancia de la temporalidad de los cargos públicos como garantía de un sistema democrático. Utilizando el ejemplo romano, comparamos lo que sucede desde la entrada en vigor de nuestra Constitución de 1978 hasta hoy, y observamos con preocupación, como la durabilidad en los mandatos de los principales órganos contitucionales se está relajando, s...

  15. The Highly Conserved COPII Coat Complex Sorts Cargo from the Endoplasmic Reticulum and Targets It to the Golgi

    OpenAIRE

    Lord, Christopher; Ferro-Novick, Susan; Miller, Elizabeth A.

    2013-01-01

    Protein egress from the endoplasmic reticulum (ER) is driven by a conserved cytoplasmic coat complex called the COPII coat. The COPII coat complex contains an inner shell (Sec23/Sec24) that sorts cargo into ER-derived vesicles and an outer cage (Sec13/Sec31) that leads to coat polymerization. Once released from the ER, vesicles must tether to and fuse with the target membrane to deliver their protein and lipid contents. This delivery step also depends on the COPII coat, with coat proteins bin...

  16. Analysis of alternatives to increase productivity and quality of airport cargo terminal through computer simulation [paper in Portuguese

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Tozi

    2012-04-01

    Full Text Available This work applies computational simulation concepts in order to evaluate the quality and productivity of logistics systems of the import cargo terminal at Campinas/Viracopos International Airport. The logistic system was simulated by use of ARENA software, which model presented consistent results, by comparison with real data, collected through field visits. The simulation results indicate that changes on the speed of storage belts and the adoption of frequency placement stock location strategy could significantly improve the indicators of waiting time (at the entrance and exit of storage, total storage time and storage productivity.

  17. Role of Air Cargo and Road Feeder Services for Regional Airports – Case Studies from the Baltic Sea Region

    Directory of Open Access Journals (Sweden)

    Beifert Anatoli

    2016-06-01

    Full Text Available Although airports play an important role the global and European economies, according to the new guidelines of the EU Commission on the new state aid rules for a competitive aviation industry (EC, 2014, it will be more difficult for unprofitable airports, to get financial public subsidies in the future. The Commission noted that the majority of regional airports experience severe problems to cover their operative costs, resulting from among other things from overlapping of airports’ catchment areas. The main objective of the above mentioned measures is not to close the regional airports, but to motivate them to operate on cost efficient, profitable and more flexible basis, stating however that and that the most inefficient airports will be still closed. In order face upcoming challenges, the regional airports shall reconsider their future development plans. Traditionally, the airports including small and regional airports focus on the passenger traffic and thus, the possible benefits and opportunities of the air cargo market are totally underestimated or even completely ignored. Most regional airports in the Baltic Sea region act totally isolated and do not have a clear picture of the current situation on the international air cargo market today, its future perspectives and sustainable development plans remain basically hidden. There are some examples when trying to meet the market demand, the regional airports have made huge, but unfortunately unjustified investments, e.g. improving airport infrastructure, runways, etc. However, the airports’ infrastructural investments alone cannot fulfil the expected “remedy” role for the airports’ sustainability. This paper aims at investigating the positioning and the role of the regional airports in the current hub-and-spoke network, focusing on the relevance and potential of the air cargo sector for the regional airports development. The optional freight value proposition for the development of

  18. Descripción de cargo para el área de mantenimiento de Perenco Colombia Limited

    OpenAIRE

    2012-01-01

    La descripción de cargo permitió establecer una estrategia de manera que los procesos de capacitación, entrenamiento y formación optimizaran la labor que desempeñan, la organización en el puesto de trabajo, la definición de objetivos, además de aplicar un modelo de carrera adecuado con las competencias a desempeñar. El presente trabajo tuvo como objetivo general el de “proponer un Modelo de Gestión por Competencias requerido por el personal de Perenco Colombia Limited”. Este estudio se desarr...

  19. ATMOSPHERE PROTECTION IN CASE OF EMERGENCY DURING TRANSPORTATION OF DANGEROUS CARGO

    Directory of Open Access Journals (Sweden)

    O. V. Berlov

    2016-02-01

    Full Text Available Purpose. The paper highlights the development of numerical models for prediction of atmospheric pollution in case of burning of the solid rocket propellant in a railway car, situated near the building on railway territory. These models can be used in predicting the effectiveness of neutralization upon the atmosphere protection for this type of accidents. Methodology.To solve this problem the numerical models based on the use of Navier-Stokes equations, to determine the velocity field of the wind flow near cars and buildings, and contaminants-transfer equations in the atmosphere were developed. For the numerical integration of pollutant transport equation was used implicit «change – triangle» difference scheme. When constructing a difference scheme physical and geometric cleavage of the transfer equation is carried out in four steps. Unknown value of pollutant concentration at each step of cleavage is determined by the explicit scheme – the method of «point-to-point computation». For the numerical integration of the Navier-Stokes equations are used implicit difference schemes. When carrying out computing experiment also takes into account: the velocity profile of wind flow; interaction between the building and the wind flow and flame jet of solid rocket propellant; the presence of a railroad car; inside which there is a source of pollution; instability of pollutant emissions. On the basis of constructed numerical models was performed the computer experiment for assessing the level of air pollution at dangerous cargo rail transportation in case of emergency at railway territory.The application calculations for the timely combustion products neutralization of solid rocket propellant were carried out. Findings. The numerical models that let promptly calculate air contamination in case of emergency during solid rocket propellant transportation, as well as calculate the rational parameters of pollutant neutralization process were developed by

  20. Re-Designed Recombinant Hepatitis B Virus Vectors Enable Efficient Delivery of Versatile Cargo Genes to Hepatocytes with Improved Safety.

    Science.gov (United States)

    Bai, Weiya; Cui, Xiaoxian; Chen, Ruidong; Tao, Shuai; Hong, Ran; Zhang, Jiming; Zhang, Junqi; Wang, Yongxiang; Xie, Youhua; Liu, Jing

    2016-01-01

    Hepatitis B virus (HBV) takes humans as its sole natural host, and productive infection in vivo is restricted exclusively to hepatocytes in the liver. Consequently, HBV-derived viral vectors are attractive candidates for liver-targeting gene therapies. Previously, we developed a novel recombinant HBV vector, designated 5c3c, from a highly replicative clinical isolate. 5c3c was demonstrated to be capable of efficiently delivering protein or RNA expression into infected primary tupaia hepatocytes (PTH), but the design of 5c3c imposes stringent restrictions on inserted sequences, which have limited its wider adoption. In this work, we addressed issues with 5c3c by re-designing the insertion strategy. The resultant vector, designated 5dCG, was more replicative than parental 5c3c, imposed no specific restrictions on inserted sequences, and allowed insertion of a variety of cargo genes without significant loss of replication efficiency. 5dCG-based recombinant HBV effectively delivered protein and RNA expression into infected PTH. Furthermore, due to the loss of functional core ORF, 5dCG vectors depend on co-infecting wild type HBV for replication and efficient expression of cargo genes. Development of the improved 5dCG vector makes wider applications of recombinant HBV possible, while dependence on co-infecting wild type HBV results in improved safety for certain in vivo applications. PMID:27171107